LOCUS       DAOTIL010000001        13784 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminobacteriaceae bacterium isolate AMR_MDS_3026
            NODE_5327_length_13784_cov_8.415544, whole genome shotgun sequence.
ACCESSION   DAOTIL010000001 DAOTIL010000000
VERSION     DAOTIL010000001.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868050
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminobacteriaceae bacterium (wastewater metagenome)
  ORGANISM  Aminobacteriaceae bacterium
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae.
REFERENCE   1  (bases 1 to 13784)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 13784)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:19:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,224
            CDSs (total)                      :: 3,198
            Genes (coding)                    :: 3,184
            CDSs (with protein)               :: 3,184
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13784
                     /organism="Aminobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5327_length_13784_cov_8.415544"
                     /isolate="AMR_MDS_3026"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:3034609"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(114..437)
                     /locus_tag="P5201_00005"
     CDS             complement(114..437)
                     /locus_tag="P5201_00005"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRV96945.1"
                     /translation="MRLLDNNPTPFVFTPFFISLAAAALRASSTISLNPRLPRFLLAF
                     LAATFKDAAVALLATFAALAPPANHPHWPTDRNISIPNRMEVRVIAPAIARQPPSSSR
                     FMRCM"
     gene            421..>12356
                     /locus_tag="P5201_00010"
     CDS             421..>12356
                     /locus_tag="P5201_00010"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRV96946.1"
                     /translation="MSSSRTQAARAFYEKLGKEVGSELTERLGGGLAAKATGKVAGAL
                     TDMLPQATMGVAQKTAIEMLGTAAMGEDPTTATHARMRLQKMFNGEDTTSEAAAIGDL
                     MIEYASELSGDYLTHMLGAGFGSMRGVGGRAGKTIASKIKAGIAGSGLDKVAGAKAFG
                     DMVGKTARRLLAGAAKTGETVRGAFDNSDFMKMITAHGYNGVIGEMLEERFGGFLRGL
                     TGLDGDEYHSYIANVWNATIPKSTDQFMAELVAFSLPAAANAVGVQTSKVMAGRADPE
                     YDRFVRIGNLTEIAGALRRKKTTDVPAPGKNAEGKKSESPIMEPTFDDVVESLVAIDR
                     LHEAEQLRHKEGYGVFGWVLDAVNAAARKVAGMQYITSTDAESLGLQKSGWRSSGYGA
                     AMRAGIVSMANESHASLESDLKTDPEYARLLEVAGRSQSQEAKQALRDRFDRLQRDQA
                     IKTAVALQAYTGLMDLSSQFSPAEIEELQAGGMSTDEVKGLSERLEKLREDPAFLDTL
                     EWDQAAKRVRFTPLALLTLDSPQLAKVLIRRNLTGFALDETSPVVREDVSGLLAALDM
                     VLDADVDGSYQTGLDYAQRLRQYLSSKDGNANLLSLIVPELSPVLNPEMVRAGSSEAV
                     GLQTGKEQLLELFDRLDPSGEARSRLHNVSRYTNPAFVSGALTFKAEELTPQDGVPTN
                     PFQVVMSRLAGNDRNVDARTYATFMAINGFNPASASHRVDFEAGLRGVLTALELGSSP
                     SELWRDKEVVARSRVDGRLKERIGVVSKIKRAKDGAVERVEVTFGQAPAEEFDPKDLS
                     PRSDVIPYVNFAFGPGSLYTTRANARLRYPDQYNQIKDVDPVRPGVDEDGTPNKDKDL
                     LRLAFGATVGPGGSITVNLFSSSHAVEDILESALRRYNEATGKKGDSPLRVPGFFLEK
                     VRRVRAEFADRLKSANDNEESAGLADDPLYQAFKEVAGAEEERAMLFELYGKLYKRHR
                     LSFKEEIDARGKPVFHALDKAADRLAALLGEEGTRQLLEEQHAFYLRFMSPEALAGLD
                     YNYRIEQAVEAATPTGQKQRRVDYGPDAAVVYVERDAKGRVAQARPGRGRLVSKGMSG
                     EKWGDAKIQNRLGTYETGFRSRPGSRSVLEVRDVNAAVKSAHGRATAAGKRYAEAQKN
                     AEKTRGETRATLREEYEGLRRKRETLSAERKGAEETLARARADEDAAAESDATKKLVD
                     VQIAYAKAGERLEQIVREEQRLDDESDSDTDLAAARLAYDAALQELTAAVAENERALR
                     PLVLDESGAERDEVDDDIPMGDPPAPTRSVIPEGTTTVEQAEALAEAYSSRGEEVPEE
                     LSRFIDTLRAARESKPKPEQKPEPESKPEPSKKKKKKPPRVRGRWSKFDGEGGQATTS
                     MLDSGEERLREAARRDGQQADELEARMRAFKGAPPDRPVDFSALDKMFESKTKQAPAK
                     AMIDALELGPEAAAALKAEVAPALSLLGKNRAKRAAAARLASLIGDMVQDGKLEGVPL
                     NQRLQDASAALQFAAATGALGDAVSGVRPVGQSGEEIDAPALASLYFLLLSHPQLSRM
                     QSALGLSSRVDPGVAATIAASLAGVPDAPGNAALDFAAMLETLTEAGADSLKGRYAAE
                     ALTKFDIARLSEVLESMLPAERDVHTEFLSDSEKAEAESLEDKVLNEFLRYRYTEDAV
                     PVVLAIVEAYEPEASVNVTGGQRGKLLDPFNKPEDYFAIVAAADREAGPGDSNEFVQV
                     LVPGQEGLEVPVPRQLAEVLRTLADAGMTHEEYRKYARRVLGVQKNIENVVARVTKKG
                     VTFAAQRRTSGAARGVARAVEGGGRSGLQDAIENANIPRLVALRDEFNRLGLREKGRE
                     EQLNEVRKAAKSPTPPKRSAEAGPVRGPELEAHRKLLQDLVLGVMDVAAEAAEWNGEF
                     GVASAIRAVANRAVRGDINYLGMLARNAVRIISEMANEGTVGARAKAFAKTSFSAIRG
                     DLSKADTDLFRSAYGFLPKGSSVLDAMIGSLFRDSSATSIRDDGSKHRTLQIPPKVLS
                     LLTAKFPSLLATEGLRPGQPALLKVDEARFGRRTSRVANLPAPARVSLALRFADRAPD
                     KSGVAWMPVNPGTREALYLVGMRTEALDKLADELLERYPNPPAEIGEQLSGHTQQGRF
                     IAAQWAMVASEFGEEAAGAALRIPDITKRIGMINGIPLSVAAPGEKIKGFVRDAVLVD
                     GVELPKAASDGWNYALAKHLTSQLGAGDGAYKINVAGHADGSVKGAAQITAAKPAGIS
                     DTVLEEHAEAQAELRRLMGDNAFDASGLDSQVLAIDAEIRAQLDEEYRGFVMDAGGEK
                     VGGTEGAVELNFTLGGKNITIRGRKAWFASEHLSKASDVDAKADRYQSAFGVSALFNI
                     GVFAPKSAERIRGLLRLGYENLAKMPEEAYAKLLGAAMASSAAKSTPASVYVVEASGL
                     AMDNHMLQSGYAGAALVREEARPRVQALYNLVAPSMQTFEEAVSGASPYLKAAVNGQP
                     ARIRLNDTRDDAMFSVWTTKEFSEKFTTEAEQRKALREFLTTLSEFRINTLSARERRT
                     MPEEALKLAEAEQKKRADKLRGMSRYFRDFTQAQSLTLLEDASGFFRADGSFREETIY
                     AAIGRDGETRLVAAPRQGMAERFPGAEGMAARDAPLVTAPATLAVRSVRVLGEDGRIK
                     RVRRVASGTQNLLVMDVASLKNRGIDNDADKHKMNFAVRPRGAAPSLSEVKKRASAGE
                     LTKNDFEWLGYWIASEFIDALDGQPDFVANAALEPDFGNGALLTIPAWLKRVAPAEFE
                     GRDKATGREMLETKLHPLAALSYPVWEARGMAAAEASDRGHVVQANDGMAAVAEHRMD
                     LSAEPGKFGADLVVKDIAPGLVLGVSGARWADLDDKLQRVYDLGNNVMNATIDAEKNW
                     TEFLHGVMADTSPMKYALVASWTPRFSRAEELKKREAFITEALVAGKKKEEGVEAYES
                     WARSTPGTTLHFEQSWVDFVETDPGMRLFLETARERQANGDAPLYSWGQWKDAMVRRL
                     ADKKNADREEGERAVTVRDEALREEVASITANVGTLYQVGDQIKRLGGVGKQYHNLGK
                     SVVDVLRMRTDLEAARKSGARLEAPDKARRLPLFPNLDMSGVQESEFLGLQTAAGSAL
                     NRAIVEHGPMSHPRILAALQRAIDEKNISKAYRIEEGIRKSLAKSAALLAFPELVDLL
                     NNEILPAYERGMAPNWFLREVYGARAEAEMEEAPEARQNRLQREAKSALADAGVFSPS
                     EETLKEKVAALDAKERDVELVADSAAPAAALDHVAATFLVALVNAKDFGQAFPALQEE
                     AARMIRTFQGVRLASSSTTKLEAAVSGPQTARAALNIDDSALDIAAWNEFLTRLEDAL
                     RESGGLKIVVGRLPRPSRIASRMARLASGFSEELSPLAKAYLNQPAGKNPSVEEIEAY
                     QERLAKYRKAQQEKLFLGSKTLDEKLSLTITADMVRPLLVYYGISSFDSMLNINAGNP
                     LPASDAATLRAFVRSFAHMASTGRLNDEVSRRLELALGAAAEQVVEAQGDMDQETVRI
                     PQDVAPVVMVDVARAADKKHRFSGRIDMAPYRFTTSMLGEMSKENFRQTRHPVRAQNG
                     EVIYVPEKYPRNDPRWAGILSGLHEADILAKLGVFHDPLVSSIKEELKSVGKPSYAKV
                     VGKEFDRMRKAARTVRSPMLKETERQRFESMHPQSLVTMERMYKEAKRRLDEAKAEPV
                     LGLGLGERIDKASLSEQRHLVASGLQKILEVKASPEWRNRMRRVRAGEEDLFSSRVFD
                     EVGLQENLAKSLRPDITNWEAAGPVFAAGGWLLAKAAERTGDSWSAKDQKIRALVADV
                     AAAAAVGDFAAAHREVELERNLSRKDRFGLDRAIVRASLDYDVRRIGDNSRTDNDMTT
                     GSLVGHWELISMYSAQRAAIGGVDYLKEEIPRLLGLVSAWENRLNTVIRAEGRKFGEG
                     RERRTIVRMVKNEKDSKKLGRDVYDYRDKIYNRAKDNLRELTDVELNE"
     assembly_gap    12357..12456
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            12487..>13784
                     /locus_tag="P5201_00015"
     CDS             12487..>13784
                     /locus_tag="P5201_00015"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRV96947.1"
                     /translation="MALATEKERKDKDSPWSKIMYNRALLRLAIRVETERAELVGAEH
                     YKIGDPTQGGTEGTAELIRGLQQSIRDYSEREWTARRGKREYPSYYDAFIQSTNDKEK
                     VSALMPAISDPLRLAETSIKDMMDTLTREVLVNQFMLAEDAAQNPFVIAIPREGVKDT
                     DPANRGRDIRSMLREDTLRKQLRMLERKAGRAGRGDTLHEKLRSLVSGNERAVNLDNY
                     ELVKSDYESIQAFYVLKKEAGYDSRARHWFQHVVGRPEQWNVEIGEEFEHDVLKTLSK
                     VNMFMKHMAVGWSLFFATAGVESTVAATPLNRNMANVFQKQFWENWLSVARLGRRVRR
                     GDLEIAMDLYKYHKAGIMISERNISDGSSSVVDEVISKVSRSLGERFGGEDDAKRAKY
                     EGRARTALRTLTGRKLSEWLMGDFFGGLKLWSTQFLIDEF"
BASE COUNT         2893 a         3851 c         4755 g         2185 t
ORIGIN      
        1 ctcggcgacg tcctcgacgc ggtggagatc gccaagatcc ttcgcatcgg agagaagctg
       61 aaggacggag acgactccga cgtcaccgac gaagagctcg tccggttcaa ctactacatg
      121 cagcgcatga agcgggagga tgagggaggc tggcgggcga tggccggcgc gatcacccga
      181 acttccattc tgtttggtat cgagatgttc ctgtccgtgg gccagtgggg gtggttcgcc
      241 ggaggcgcta aggccgcgaa ggtggcaagc aaggccaccg cggcgtcctt gaaagtggcg
      301 gccaagaagg ccagcaagaa tctcggcaag cgggggttca gagagatcgt cgaagacgcc
      361 cgcaacgcgg ccgcggccaa gctgataaaa aatggagtga agacgaaggg cgttgggttg
      421 ttgtccagca gtcgcacgca ggcggcccgg gccttctacg agaagctcgg caaggaggtc
      481 ggcagcgagc tgactgaacg gctcggcggc gggttagccg cgaaggcgac ggggaaagtc
      541 gccggcgcgc tgacggacat gctcccgcag gccaccatgg gagtggcaca gaagaccgcg
      601 atcgagatgc tgggcactgc ggcgatggga gaggatccga ccacggccac gcatgcccgc
      661 atgcgactgc agaagatgtt caacggggaa gacacgacga gcgaagccgc ggcgatcggc
      721 gaccttatga tcgagtatgc gtccgagctc tccggcgact accttaccca catgctgggg
      781 gcaggattcg gaagcatgcg cggagtcggc ggaagggccg gcaagacgat cgcatccaag
      841 ataaaggccg ggatcgcagg atccggtctt gacaaggtgg ccggggcgaa ggccttcggc
      901 gacatggtcg gtaagaccgc caggagacta cttgccgggg ccgcgaagac gggggagacc
      961 gtgcggggcg cgttcgacaa ctccgacttc atgaagatga ttacggcgca cgggtacaac
     1021 ggtgtgatcg gggaaatgct cgaagagcgg ttcggcgggt tcctgcgcgg gcttaccggg
     1081 ctcgacggcg acgagtacca ctcgtacata gcgaacgtgt ggaacgccac gatcccgaag
     1141 agcaccgacc agttcatggc cgaactggtg gcgttctctc tcccggcggc ggccaacgcc
     1201 gtgggcgtgc agacctcaaa agtcatggcc ggcagggccg accccgagta cgatcgcttc
     1261 gtcaggattg gcaatctgac cgagatcgct ggggcgctcc gtcgcaagaa gacgacggac
     1321 gtccccgccc ccggcaaaaa cgcagagggc aagaagtcag aatccccgat catggagccc
     1381 acgttcgacg acgtcgtgga gagcctcgtc gcgatcgacc gcctgcacga ggcggagcag
     1441 ctgcggcata aagaagggta cggcgtattc gggtgggtgc tggatgccgt gaacgcggct
     1501 gcgcgcaaag tggcgggcat gcagtacatc acgagtacag atgcggagtc gcttggtctg
     1561 cagaagtccg ggtggaggtc ttccggctat ggtgcggcga tgcgcgccgg catcgtgtca
     1621 atggctaacg agtcccatgc gtctttggag tccgatttga agacggaccc ggagtacgcc
     1681 aggctgctgg aagtggcggg caggagccag tcccaggaag ccaagcaggc acttcgcgat
     1741 cggtttgacc gcctgcaaag agaccaggct atcaagaccg ccgtcgcgct tcaggcgtac
     1801 acggggctca tggacctgtc ttcccagttc tcgccggcgg agatcgagga gctgcaggcc
     1861 ggggggatgt cgacagacga ggtgaagggg ttgtcggagc ggcttgagaa gctcagggag
     1921 gacccggcgt tcctggacac tttggaatgg gatcaggccg cgaagcgcgt gcggttcact
     1981 ccgttggcgt tgctgacgct ggattccccc cagctcgcca aggtcttgat ccgaagaaat
     2041 ttaaccgggt ttgccctgga cgaaaccagc cccgttgttc gggaggacgt gtccggactc
     2101 ctggcggctt tggacatggt gctcgatgca gacgtcgatg ggtcttacca gaccgggctg
     2161 gattacgcgc agaggctaag gcagtatttg tcgtcgaaag acggcaatgc aaatctgtta
     2221 agtctcatcg ttcctgagct gtcccccgtg ctcaacccgg agatggtccg cgcggggtcg
     2281 agtgaggcag tggggctgca gaccggaaaa gagcagttgc ttgagctgtt cgatcggctg
     2341 gacccttcag gcgaagctcg ctcccgcctg cacaacgtgt cgcggtacac caacccggcc
     2401 ttcgtgtccg gggcgttgac tttcaaagcg gaggagctga cgccgcagga cggcgtcccg
     2461 acgaatccgt tccaggtcgt aatgtcacga cttgcaggca acgacaggaa cgtggacgcc
     2521 cgcacgtacg cgaccttcat ggcgatcaac gggttcaacc cggcgtcggc ttcacacaga
     2581 gtggacttcg aggccgggct tcgcggcgtc ctgacggcct tggaactcgg gtcttccccg
     2641 agcgagctgt ggagggacaa agaggtggtc gccaggtccc gcgtggacgg ccgtctgaaa
     2701 gagcggatcg gagtcgtgtc caagatcaag cgggcgaagg acggcgccgt ggagcgggtc
     2761 gaggtcacgt tcggccaagc gcctgcggaa gaattcgatc ccaaagactt atcccctcgg
     2821 tcggacgtca taccatatgt caatttcgcg ttcggtcccg ggtctctgta cacgacgcgg
     2881 gcgaacgccc gcctgcgcta tcccgaccag tacaaccaga taaaggacgt ggacccggtc
     2941 cgacccggag tggacgaaga cggcaccccg aacaaagaca aggacttgct tcggctggcg
     3001 ttcggggcaa ctgtagggcc ggggggctcc ataacggtca acctgttctc ctcctcgcac
     3061 gccgttgagg atattctgga gtctgcgctt cggagataca acgaagccac cgggaagaag
     3121 ggggactccc cgcttcgagt cccgggattc tttttggaaa aggtgcggcg cgtccgcgct
     3181 gagtttgcag acaggttgaa atccgcgaac gacaacgagg agtcggccgg gctcgcggac
     3241 gacccgttgt accaggcgtt caaggaggtg gcgggggccg aggaagagcg ggccatgctg
     3301 ttcgagctgt acggcaagct gtacaagcgg caccggctaa gcttcaagga agaaatagat
     3361 gcgcgaggga agcccgtgtt ccacgccttg gataaggcgg cggaccggct ggccgcgttg
     3421 cttggtgaag aggggactcg gcagctgttg gaggaacagc acgcgttcta ccttcgattc
     3481 atgtccccgg aggcgcttgc cggtctcgac tacaactacc gtatcgagca ggcggtggag
     3541 gcggccaccc ccaccggcca gaagcaaagg cgtgtcgact atggcccgga cgccgcggtg
     3601 gtctacgtgg agcgcgacgc gaagggacgg gtcgcccagg cccgcccagg acgcggcagg
     3661 ctcgtatcga aggggatgtc cggagagaag tggggcgatg caaaaatcca aaaccgcctg
     3721 ggcacctacg aaacgggttt tcggagccgc ccgggcagtc gcagcgtgtt ggaggtccgc
     3781 gacgtaaacg ccgcggttaa atctgctcac gggcgggcga cggcggccgg taaacgatac
     3841 gcggaagcac agaagaatgc ggagaagacc cgaggggaga cccgggcgac tctgcgggag
     3901 gagtatgagg ggctgcgcag gaaacgagag accctgagcg cggaacgaaa aggcgcggaa
     3961 gagacgttgg ccagggcgcg ggccgacgag gacgcggccg ccgagagtga cgcgacgaaa
     4021 aaattggtcg acgtccagat tgcgtacgcg aaggccggag agcggctcga gcaaatcgtt
     4081 cgagaagagc agcgcctcga cgacgaaagc gactccgaca cggacctcgc tgccgcgagg
     4141 ctcgcttatg atgcggcgtt gcaagagctg actgccgcgg tggcggaaaa cgagcgcgcg
     4201 ctgcggccgc tggtattgga tgagtccggc gccgagcggg acgaagtaga tgacgacata
     4261 ccgatggggg acccgccggc cccgacccgt agcgtcattc cggaagggac tactaccgta
     4321 gaacaggcgg aggcgttggc ggaggcctat tcttcgagag gggaagaagt tccggaagag
     4381 ctttctcggt ttattgacac cctgcgggct gcgagggagt cgaagccgaa gccggagcag
     4441 aagcccgagc cggagtcgaa gccggagccg agtaagaaga aaaagaagaa gccccctaga
     4501 gtgaggggcc ggtggtcaaa atttgatggc gaggggggac aggcaaccac ctccatgctg
     4561 gatagcgggg aggagcggct gcgcgaagcg gcccgcagag acggccagca ggccgacgag
     4621 ttggaggctc gtatgcgagc gttcaagggg gctccgccgg accgccccgt ggacttctcg
     4681 gcgctggata agatgtttga gtcgaagacc aagcaggctc cggctaaagc catgatagac
     4741 gctttggagc tggggccgga ggcggcagcc gccctgaagg cagaggtggc ccccgcgttg
     4801 tcgctcttgg ggaagaaccg ggcgaagcgc gcagccgccg cccgcctggc ctcgcttatc
     4861 ggcgacatgg tgcaagacgg caagctcgaa ggggtgccct tgaaccaaag gctccaagac
     4921 gcttctgccg ctttgcagtt cgcggcggcc accggcgcgc tcggcgacgc agtaagtggt
     4981 gtgcgccccg tcggccagag cggggaggaa attgacgctc cggcattggc ctctctgtac
     5041 tttctgctgc tgtcccaccc gcagctgtca aggatgcagt ccgcgctcgg gctttcctcc
     5101 cgcgtagacc cgggggtggc cgcaacaatc gcggcgtctc tagcgggagt cccggatgca
     5161 ccaggaaacg cggcgctcga cttcgccgcc atgctggaaa cgctgaccga agcaggcgcc
     5221 gattcgctga agggccggta tgcggccgag gcgcttacga aatttgacat cgctcggctg
     5281 tctgaagtct tggagtccat gctcccggcc gagcgcgacg tgcatacgga gttcctaagc
     5341 gacagtgaaa aagcagaggc cgagtcgctc gaagacaaag tgctcaacga gttcctgcgg
     5401 taccggtaca ccgaggacgc cgtccccgtg gtcttggcca tcgtggaggc gtacgagccg
     5461 gaagcgtccg tgaacgtcac cggagggcag cggggcaagc tgctcgaccc gttcaacaag
     5521 ccggaagact acttcgcgat cgttgccgct gcggataggg aggccgggcc gggggactcg
     5581 aacgaatttg tacaggttct ggtgcctggg caggaagggc ttgaagtgcc ggttccccgc
     5641 caactcgccg aggtgcttcg cacgctggcc gacgcaggca tgacacacga agagtaccgg
     5701 aagtacgcac gtcgtgtcct gggggtgcag aagaatatcg agaacgtggt ggcaagagtg
     5761 acgaaaaagg gcgttacttt tgccgcgcag cgtaggacgt ccggagcggc ccgcggagtc
     5821 gcccgcgcgg tggaaggtgg cggtcgctcc ggtctccagg acgcaatcga aaatgcaaat
     5881 ataccgcgcc tggtggcctt gcgggatgag ttcaaccggc ttgggctccg cgagaagggc
     5941 cgcgaggagc agctaaacga ggtgcgaaag gcagccaaaa gcccgacgcc gcccaagcgc
     6001 tctgcggaag cgggcccggt aagagggccg gagctggaag cgcacagaaa actgttgcag
     6061 gatctcgtac ttggagtcat ggacgtcgcg gcggaagctg cggagtggaa cggcgagttt
     6121 ggggtggcct ctgcgatacg tgcggttgcg aatcgcgccg tccgcggtga tatcaactac
     6181 ctcgggatgc tggccaggaa cgctgtgcgc atcatttccg agatggcgaa cgagggcacg
     6241 gtaggcgcgc gcgcgaaggc gttcgcgaag acttcgttct ccgccatccg cggcgatctg
     6301 tcgaaggcgg acactgacct gttcaggtcc gcgtatgggt tcctcccgaa aggcagctcc
     6361 gttcttgacg cgatgatcgg atccctgttc cgcgactcca gcgcgacgtc cattcgcgac
     6421 gacgggtcca agcaccgcac gttgcagatc ccgcccaagg tgctttcatt gctcaccgcc
     6481 aagttcccaa gtctcttggc gactgaggga cttcgccccg ggcagcccgc cctgttgaag
     6541 gtcgatgagg ccaggttcgg gcgcaggacg tcccgcgtag caaatctccc ggcccctgcg
     6601 cgagtcagcc tagccctgcg attcgctgac agggccccgg acaagtccgg cgtggcgtgg
     6661 atgccggtga accccggcac acgcgaggcg ttgtacttgg ttggcatgcg gacggaagcg
     6721 ctggacaagc tggcggacga gctgttggag cggtatccga acccccctgc cgagatcggg
     6781 gagcaactgt ccgggcacac acagcaggga agattcattg ccgcccagtg ggccatggtg
     6841 gccagcgagt tcggggaaga ggccgccggg gcggcgctgc gaatccccga catcacgaag
     6901 cggatcggaa tgatcaacgg gattcctctg tccgtcgcag ccccggggga gaagatcaag
     6961 ggcttcgtcc gggacgcggt gctcgtcgac ggtgtcgaac ttccgaaggc cgcgtccgat
     7021 ggctggaact acgccctcgc gaagcatctt acgagtcagc tcggcgcggg cgacggcgcc
     7081 tacaagatca acgtggcggg gcacgccgac ggctccgtga agggcgccgc acagatcacc
     7141 gcggccaaac cagccggaat ctctgacacc gtgttggaag agcacgcgga ggctcaggca
     7201 gagctgcgca gactgatggg ggacaacgcg ttcgacgcct ccgggctgga cagtcaagta
     7261 ctcgccatcg acgcggagat ccgggcccag ttggacgagg aataccgcgg gtttgttatg
     7321 gacgccgggg gcgaaaaagt cggtgggacg gagggagccg tcgagctgaa cttcacgctc
     7381 gggggtaaga atataacgat tcgcgggcgc aaggcctggt tcgcaagcga gcacctttcg
     7441 aaagcatcgg acgtcgacgc gaaggcagac cggtaccagt ccgccttcgg ggtaagcgcg
     7501 ctgttcaata tcggagtgtt cgcgccaaag tcggctgagc gcattcgcgg gctgcttcgc
     7561 ctcgggtacg agaacctagc caagatgccg gaagaagcgt acgccaagct gctcggagcg
     7621 gccatggcgt cgtcggctgc gaagtcgact ccggcttccg tatatgtcgt cgaggcgtcc
     7681 ggactcgcga tggacaacca catgttgcag tccgggtacg ccggcgccgc gctggttcgc
     7741 gaagaggccc gcccgcgtgt ccaagcattg tacaacctgg tggctccttc catgcagacg
     7801 ttcgaagagg cggtatccgg ggcgtcgccg tacttgaagg cggcggtcaa cggacagccc
     7861 gcccggatac ggctcaacga cacacgggac gacgccatgt tctcggtgtg gacgacgaaa
     7921 gagttttccg aaaagtttac gacggaagcg gagcagcgga aggcgctgcg ggagttcctg
     7981 acaacgctgt ccgagttccg tatcaatacg ctatccgcca gagagcgacg cacgatgccg
     8041 gaagaggcgc tgaagctggc cgaggcggag cagaagaaac gcgcggacaa gctccgcggc
     8101 atgagccggt acttccggga cttcacgcag gcccaatcgc tgacccttct ggaggacgct
     8161 tcgggattct tccgggcaga cggctccttc cgggaagaga ctatctatgc ggcaatcggc
     8221 cgggacgggg agacgaggct tgtcgccgcc ccgcgccagg gaatggccga acggttccct
     8281 ggggccgagg ggatggctgc gagagacgcc ccgcttgtca cggctcctgc tacgctcgca
     8341 gtgcgctccg tccgggtgct gggggaagac ggccgcatca agcgtgtccg ccgggtggcg
     8401 tctgggacgc agaacctact tgtgatggac gtggcttctc tgaagaaccg cggcatcgac
     8461 aacgacgccg acaagcacaa gatgaatttc gcggtgcgcc ccaggggcgc ggcgccgtct
     8521 ttgtccgaag tgaagaagcg cgcttccgcg ggcgagctca ccaagaacga ctttgagtgg
     8581 ctggggtatt ggatagcgtc cgagttcatc gacgcgctcg acggccagcc ggatttcgtg
     8641 gcgaacgctg ccctggagcc agattttgga aacggggcgc tgctcaccat cccggcctgg
     8701 ctgaagcgtg tggccccagc cgagttcgaa ggccgcgaca aagcgaccgg ccgggagatg
     8761 ctggagacca agctccaccc cctggcggcg ctgtcctatc cggtatggga agcgcgtggt
     8821 atggcggcgg cggaggcgag tgatcgtggg cacgtggtgc aggccaacga cggcatggcc
     8881 gctgtggcgg agcaccggat ggacctgtct gcggagcccg gcaagttcgg tgcggacctc
     8941 gtcgtaaagg acatcgcccc cgggcttgtt ctcggagtca gtggcgcgcg ttgggccgac
     9001 ttggacgaca agctgcagcg cgtgtacgac ctcggaaaca acgtgatgaa cgcgaccatc
     9061 gacgcggaga agaactggac ggagttcctc catggggtga tggcggatac gagcccgatg
     9121 aagtacgcgc tcgtcgcttc gtggacgccg cgcttctcca gggccgaaga gctgaagaaa
     9181 cgagaggcct tcataacgga ggccctggtc gccgggaaga aaaaagaaga aggagtggag
     9241 gcctacgaga gctgggcccg gagcacgccg gggaccaccc tgcacttcga acagtcgtgg
     9301 gtcgacttcg tggagacgga ccccggaatg cggctcttcc tggagacggc gagggagcgg
     9361 caggcgaacg gcgatgcgcc gctgtactct tgggggcagt ggaaagacgc catggttcgg
     9421 agactggcgg acaagaagaa cgccgaccgc gaagaagggg agcgcgcggt gacagtaaga
     9481 gacgaggccc ttcgcgaaga ggtagcctct atcacggcga acgtcgggac gctctaccaa
     9541 gtcggagacc agatcaagcg cctgggcggg gttggtaagc aatatcacaa tctcgggaaa
     9601 tcggtggtcg acgtgctgcg gatgcgtacc gatctcgaag cggcgcgcaa gagcggggcc
     9661 cgactggagg cgcccgacaa ggcgaggcgt ctcccgctgt tcccgaacct ggacatgagc
     9721 ggggtgcagg agtcggagtt cctcgggctg cagacggcgg cgggctccgc gctgaaccgg
     9781 gcgatcgtcg agcacgggcc catgagccat cccaggattc tcgcggccct gcagcgggca
     9841 atcgacgaaa agaatatatc gaaagcgtac cgcatagaag aaggaattcg gaagtctctc
     9901 gcgaaaagtg cggcgctgct ggcgttcccc gagctcgtgg atctgctcaa caatgagatt
     9961 ctgcccgcgt acgagcgcgg gatggcacca aactggttcc tgcgagaagt gtacggggcc
    10021 agagcggaag ccgagatgga ggaggctccg gaagcgcgcc agaacaggct gcagcgggaa
    10081 gcgaagtcgg cgctggcgga cgccggcgtg ttctctccaa gcgaagagac cttgaaggaa
    10141 aaggtcgctg cgctggacgc gaaagaacga gacgtcgagc ttgttgccga ctctgccgcc
    10201 ccggctgcgg cgttggacca cgtcgccgct actttcctgg ttgccttagt gaacgcgaag
    10261 gatttcgggc aggcgttccc cgcgctccag gaagaggccg cgaggatgat ccgaaccttc
    10321 cagggggttc ggttggcttc cagctcgacg accaagctcg aagccgcggt gtctggaccg
    10381 cagacggcgc gggccgcttt gaacatcgac gactccgcgt tggatatagc ggcctggaac
    10441 gaattcctga cgcgccttga agacgccctc cgcgagagcg gcggcctgaa aattgttgtg
    10501 ggacgactgc cccgcccgtc acgaattgca tcgcgcatgg cgcgcctggc gagcggattc
    10561 tccgaagagc tctccccgct ggccaaggcg tacttgaacc agccggccgg taagaatccg
    10621 tccgtggaag aaatagaagc gtaccaagaa cgcctggcga aataccgcaa ggcgcagcaa
    10681 gagaaactgt tcctcggcag caaaacgctg gacgaaaaac taagccttac cataaccgcg
    10741 gacatggttc gtccgctatt agtgtactac gggatctcgt cgttcgattc catgctgaac
    10801 atcaatgccg ggaatccgct gcccgcgtct gacgcggcca cccttcgcgc cttcgtgcgc
    10861 agcttcgcgc acatggcgtc cacgggccga ctgaatgatg aagtgtcgcg tcgtctggag
    10921 cttgctcttg gcgcggctgc ggagcaggta gtcgaagccc aaggagacat ggaccaggaa
    10981 accgtgcgca tcccgcagga cgtggctccc gtcgtaatgg tagacgtggc ccgcgcggca
    11041 gacaagaaac accggttctc cggacgcata gacatggctc cgtatcgatt caccacgtcg
    11101 atgctgggag agatgtcgaa ggagaacttc cgtcagaccc gacacccggt tcgtgcgcag
    11161 aacggagagg tgatctacgt ccccgagaag tacccaagaa acgacccgcg gtgggcgggc
    11221 atcctgtccg ggctgcacga ggcggatatc ctggccaagc tcggagtgtt ccacgacccg
    11281 ctggtaagct ccatcaaaga ggagctcaag agcgtaggca agccgtcgta tgcgaaagtc
    11341 gtcgggaaag aattcgacag aatgcgcaag gcggcccgga ccgttcgatc gccgatgctc
    11401 aaagagacag agcgacagcg tttcgaatct atgcacccgc agtcccttgt caccatggaa
    11461 cgaatgtata aagaagccaa gcgacgcttg gacgaagcga aagcggagcc cgtgcttggg
    11521 cttgggctcg gagagaggat agataaggcg agtctgtcag aacaaaggca cttggtcgct
    11581 agtgggctgc agaagatttt ggaagttaag gcgagcccgg agtggcgcaa tcgaatgcgc
    11641 cgcgtgcgcg caggggaaga agacctgttt tcaagccgcg tgttcgacga agtaggcctc
    11701 caggagaacc tggccaagtc gcttcgtcca gacatcacga actgggaagc cgcgggcccg
    11761 gtgttcgccg ccggcgggtg gcttcttgcg aaagctgcgg agcgaactgg ggactcgtgg
    11821 tcggcaaaag atcagaagat acgcgcgctg gtagctgatg tggctgcagc ggctgcggtt
    11881 ggtgacttcg ctgccgcgca cagagaagta gaactcgaac gaaatctatc gcgaaaagat
    11941 cggttcgggc tggaccgggc gatcgttcgt gcttcgctgg attacgacgt acggagaata
    12001 ggcgacaatt cacgtaccga caacgacatg acgacggggt ctttggttgg gcattgggag
    12061 ctcatttcaa tgtactccgc tcagcgagct gccatcggcg gggtggatta cttgaaagaa
    12121 gaaattccgc ggctgctcgg gctcgtgtcc gcttgggaga accgcctcaa cacggtcatc
    12181 cgtgcggagg gccggaagtt cggggagggg cgggagcgcc ggactatcgt tcgaatggtg
    12241 aagaacgaga aggactctaa gaagctgggg agggacgtct acgactaccg ggacaagata
    12301 tacaaccggg caaaggacaa cctacgagaa cttacggacg tcgagctgaa cgaacannnn
    12361 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    12421 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnntacc gaaagaagaa aaagaaggcc
    12481 ccgagaatgg cgctggccac ggagaaggag cgcaaagaca aggacagccc gtggagcaag
    12541 atcatgtaca accgggcgct ccttcgcttg gctatccgcg tggagacgga gcgggcggag
    12601 ctggtcgggg cggagcatta caaaatcggg gacccgacgc agggcggcac cgaaggaact
    12661 gcagaactta tccgggggct gcagcagtcc atacgagact actccgaacg ggagtggacg
    12721 gctcgtcgag gaaagcggga gtacccgtca tactacgacg ctttcataca gtccacaaac
    12781 gacaaggaaa aagtgtccgc gctgatgccc gcgatatcgg acccgctgcg tctcgcagag
    12841 acgtccatca aagacatgat ggacacgctc acgagggaag tcctcgtgaa tcaattcatg
    12901 ctggccgagg acgccgcgca gaatccgttc gtcatcgcga tcccgagaga gggggtcaaa
    12961 gataccgacc cggcgaatcg cggaagagac atccgcagca tgctgcggga ggacacgctt
    13021 cgaaaacagc ttcggatgct ggagcgcaag gcggggcgtg ccgggcgcgg ggacacgctg
    13081 cacgagaagc tccgcagcct ggtcagcggg aacgagcgcg ccgtaaacct cgacaactac
    13141 gagctggtca agagcgatta cgaatctatc caggctttct acgtgttgaa gaaagaggcg
    13201 gggtacgaca gccgggcgcg gcattggttc cagcacgtcg ttgggaggcc ggagcaatgg
    13261 aacgtggaga tcggggagga gtttgagcac gacgtgttga agacgctgtc gaaggtcaac
    13321 atgttcatga agcacatggc cgttgggtgg agcctgttct tcgcgaccgc cggcgtcgag
    13381 tcgaccgtgg ccgccacccc tctgaatagg aatatggcca acgtatttca gaagcagttc
    13441 tgggaaaact ggttgtcggt agcaaggctc ggccgaaggg tgcggcgcgg agacctcgag
    13501 atcgccatgg acctgtacaa gtaccacaag gccggcatca tgatctcgga gcgcaacatc
    13561 tctgacggct ccagctcggt ggtggacgaa gtaatatcca aagtgtcccg ctcgcttgga
    13621 gagcggttcg gaggagaaga cgacgccaaa agagccaagt acgagggccg ggcgcggacc
    13681 gcgctgcgca cgctgaccgg acgcaaactg tctgagtggt tgatggggga cttcttcggc
    13741 ggactgaaat tgtggtcgac gcaatttctt atagacgagt tcca
//
LOCUS       DAOTIL010000002        13590 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminobacteriaceae bacterium isolate AMR_MDS_3026
            NODE_5413_length_13590_cov_3.823938, whole genome shotgun sequence.
ACCESSION   DAOTIL010000002 DAOTIL010000000
VERSION     DAOTIL010000002.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868050
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminobacteriaceae bacterium (wastewater metagenome)
  ORGANISM  Aminobacteriaceae bacterium
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae.
REFERENCE   1  (bases 1 to 13590)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 13590)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:19:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,224
            CDSs (total)                      :: 3,198
            Genes (coding)                    :: 3,184
            CDSs (with protein)               :: 3,184
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13590
                     /organism="Aminobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5413_length_13590_cov_3.823938"
                     /isolate="AMR_MDS_3026"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:3034609"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..790
                     /locus_tag="P5201_00020"
     CDS             <1..790
                     /locus_tag="P5201_00020"
                     /inference="COORDINATES: protein motif:HMM:NF019522.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="YibE/F family protein"
                     /protein_id="HRV96948.1"
                     /translation="IPAVAGFITLACAALAVLAGTSGVKALCGLFLSLFLLLGWFVPM
                     LAEGYTPVPFAILAIAGVSLLTIVFVVPKRALWPIPFIGAVGGAIGASVTGWIMVGLW
                     QLTGLENDSAVLLFSVAPHLELRGLLLAAVMVGGIGAVLDVAVSVTSSMAELYSYDPS
                     IPLRRLWRAGINVGSDVLGSMINTLILAYLGSSLPFVVLISMEGPDLVALLNDPHIAQ
                     EILRSVAGTVGLLLTIPVTAGAGVWWIARSHIPDEKRHNEELAD"
     gene            859..1866
                     /gene="gap"
                     /locus_tag="P5201_00025"
     CDS             859..1866
                     /gene="gap"
                     /locus_tag="P5201_00025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011897301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA];
                     GO_process: GO:0019682 - glyceraldehyde-3-phosphate
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HRV96949.1"
                     /translation="MKKIAINGLGRIGRLVLRCYMANKPADVEIVALNDLTPASEMAY
                     LIKFDSIHRKANFSVEARENSLVLDGKEIPLFKEKDPAKLPWKELGVDIVLECTGFFT
                     KREKAMAHIEAGAKRVIISAPAEDSDLTIVLGVNENMYDPAKHVVVSNASCTTNSLAP
                     VTKILNDAFGIEYLMGTTIHAYTSTQVLVDVPKGGGRKGRAAAVSLVPATTGAAKAMV
                     PLFPELKGKMDMISVRVPVADGSLTDIVVHFKKEVTVESVNAALKSAADGKLKGIVEY
                     NDEEIVSADIIGNTHSGIVDAPSTKVIMGKVAKVMVWYDNEYGYSNRMVELAQYIAGK
                     E"
     gene            complement(1974..3659)
                     /locus_tag="P5201_00030"
     CDS             complement(1974..3659)
                     /locus_tag="P5201_00030"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing phosphohydrolase"
                     /protein_id="HRV96950.1"
                     /translation="MREFSIPRSALPPGSIILGDPFYNIQEYTPFIFANLFLLLFLAG
                     LAVYLKRKINRRKKTEEELLRQTEYWKKLFEHSPQGILIYDGAGQVKEANSRFREMFR
                     LSGPAPLPENITSLLSGTEADFSRNDIFPQEENISGTAAPAKETALPDGDGGTLSVSF
                     QAFPLSSNDEETHYCALFHDIRDRKTMEQKLHRRTILQERMTAISSRFVLFQNFRDSM
                     EAALEDILALSGARTASLFVISDSGILIPEMEAFSPDKAGPSLAANFSDGDDFFWKSL
                     LLNDALPSVITIESAVHKVRESKWDFLKEMGIDSITILPLFVKTSFQGFLALTDPLQQ
                     WVDMSNDPLLGLFSDLMAMAIERRKEEDSLRETHERIHDRFTGAITALCQVSELRDVS
                     TSGHQKNVSVLAEGLANEMQLPEEVRMGVRYAGLVHDLGKLYIPAEILGKPSRLSEAE
                     YELVKKHPEFGHDILSLLNFPWPLAEIVLQHHERVDGTGYPYSLKRKDIRIEARILSV
                     ADAFEAMTSDRPYRKRKTVKAALKELIDLAGKAYDAKIVKSFVKMILERGENP"
     gene            complement(4647..5900)
                     /locus_tag="P5201_00035"
     CDS             complement(4647..5900)
                     /locus_tag="P5201_00035"
                     /EC_number="1.14.13.-"
                     /inference="COORDINATES: protein motif:HMM:NF015451.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HRV96951.1"
                     /translation="MSAPQIVVIGAGPAGLIAAGRAAEKGARVILVEKNQTVGEKLLI
                     SGKRRCNLTNAEEDMDVFLSKYGPNGKFLYSSFAGFGPRETVSFFQSRGVPLKEERGK
                     RFFPATGGSEGVLAALLNYVREGGVTIYRKRESINLEIRDGRVRRFILRGQEVEGDAF
                     IICTGGKSFAKTGSTGDGYRFARRAGHTVIPPVPALCPVRTVEKWPLASRGMTLRNVS
                     LTLWKNDEVASQRFGELLLTHFGISGPIAMDMSKEIAAAMETGTARLTLDLKPALSLP
                     VLKNRIGRDFEKFSGRKFKDSLGELLPRGLIPAVLALKPLPEDKIVNSASNQEIETLA
                     ELLKAFPLTPDGLLGFDWAIVTSGGVALKEIEPRTMRSKLVENLFFAGEVLDLDGPTG
                     GFNLQMCWSTGYQAGESAADLSLPR"
     gene            complement(5897..8029)
                     /locus_tag="P5201_00040"
     CDS             complement(5897..8029)
                     /locus_tag="P5201_00040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008522960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003729 - mRNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Tex family protein"
                     /protein_id="HRV96952.1"
                     /translation="MNTEICERVSKELKLPRDSVHAVLLLLEKDCTVPFIARYRKEAT
                     GSLDEEAVLAIKERSIRLEELEARRSAIHASLTDRGLLNPELEKAIANAASLTALEDI
                     YLPYRPKRKTRASIAAEKGLAPLADLLLRQEGNDPFSLAISFLSEEKGVATEEEALQG
                     AKDIIAERISENREARRQVRILFGRRGKLKSSCIKGKEEEGANFKDWFAWEEPAALAP
                     SHRILAMFRGEREKVLSLSLLPPEEEALRILRDIFVHGVGPDSILVNEAIIDGYRRLL
                     APSMETELRNALKKKADREAIAVFAKNVREILLASPLGPRNILAVDPGFRTGCKFVCL
                     NRQGDLLFNGTIFPHPPRADEAKSAEIMRKAIDEYGIEAVAIGNGTAGRETERFFRGL
                     GLPSDILITMVNESGASIYSASETARREFPEHDLTVRGAVSIGRRLLDPLAELVKLDP
                     KSIGVGQYQHDVDQKELKNALATVVESCVNAVGVELNTASLELLSFVSGIGSGTADRI
                     IKFREANGPFSSRKELLNVPRLGPKTFEQAAGFLRIRGGTNPLDASAVHPERYPLVER
                     IAADLGCSLPDLLSSPTMKNKIDVKRYISEDAGLPTLTDILNELEKPGRDPRETFEPF
                     TFQEGVEKIDDLLPGMPLPGIVTNITSFGIFVDVGVHQDGLVHRKRLPHGKEFRPGER
                     VNVSVLEVDRPRKRISLSMREEKKGTNR"
     gene            8147..8770
                     /locus_tag="P5201_00045"
     CDS             8147..8770
                     /locus_tag="P5201_00045"
                     /inference="COORDINATES: protein motif:HMM:NF012376.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HRV96953.1"
                     /translation="MGRNTWLPLYRRLVLGLSLPVDAEALRGKVALHFSDTPSTFYGV
                     MRRLIRFFEPQCIVHTGDLADDVKLEIRPGELPLYRRKLMELTSVLSSVQKSRIVLVT
                     GNHDHEESVREFFPDSSVIIKSGRIEMCGLDFNLSHEPEGLQTPYGHFNLFGHAPVPE
                     EAPSQGQVFLNGLYAIHVLRVDTGEVLALEYPGYVNDARLNKRRVGL"
     gene            8792..9814
                     /locus_tag="P5201_00050"
     CDS             8792..9814
                     /locus_tag="P5201_00050"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRV96954.1"
                     /translation="MLSIVRGGPRVLFLKGERSALDHALSAAFSVTRHTFESALDCAG
                     EGQTIVALHGKEDDGGREFRIVSSSSDEVLAALVNDSSCRLESVRLLPRILFFRIFGD
                     KERVLDQIENDLAGGGIPGATVRRGRLRALLGSSRRNEIAVCFTYAPLNGLIAVADML
                     NDVLFVEGTFFYDLNVFLRSRGLWYFSEGLENRSWNEMEIRIYDSWGFFMEHAERLRA
                     LLGSLEIGMILGEGWGKDFAHILMPVQIYRLRLFTFLSTLLVKELLMGLEYAENGDRL
                     ADFDLYSGRDKISWGSLKDRGAGRANLGKTAREKLFSRLSVRDRERLTAMEHRLLRLR
                     QGGNNG"
     gene            10099..10359
                     /locus_tag="P5201_00055"
     CDS             10099..10359
                     /locus_tag="P5201_00055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009200339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stage V sporulation protein S"
                     /protein_id="HRV96955.1"
                     /translation="MEVLKVSAKSQPKSVAGAIAAVLREKGAVEVQAVGAGAVNQSVK
                     SIAIARGYVAPNGIDLVCIPAFSKISIDDEERTAIKFQLESR"
     gene            10572..11852
                     /gene="hisS"
                     /locus_tag="P5201_00060"
     CDS             10572..11852
                     /gene="hisS"
                     /locus_tag="P5201_00060"
                     /EC_number="6.1.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005661519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004821 - histidine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006427 - histidyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine--tRNA ligase"
                     /protein_id="HRV96956.1"
                     /translation="MAEMSAPKGVRDILPEESWKWAYVFRMVQETARHFGYSEVHLPI
                     FEHTELFSRGIGDTTDVVEKEMYTFLDKGDRSITLRPEATASMVRAYLEHRMGTMAQP
                     VKMWCAGPMFRYERPQKGRYRQFWQVDFESIGASSPYADVEIIAISLELFRRLGLRHL
                     EVVINSVGCPECRPDYRKRLTDYFESRKDNLCETCLGRLERNPLRILDCKRDGCKAVT
                     EEAPDVYDSLCGECREHFDVVKDSLDALGASYRLDKRLVRGLDYYTKTAYEILSGELG
                     SQNAVCGGGRYDNLAEAIGGPHVPGVGFAAGVDRIVMVMEQQECSFGSVPTVEVFAVA
                     LDDISRRNVLQLVDMLRRNGIVAEMDLAGKSMKAQMKAASSRGARFACILGESERTRG
                     TVMVKDLATAEQQEITLSLLVAELKNKLTSTRKG"
     gene            11876..>13590
                     /gene="aspS"
                     /locus_tag="P5201_00065"
     CDS             11876..>13590
                     /gene="aspS"
                     /locus_tag="P5201_00065"
                     /EC_number="6.1.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006300582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate--tRNA ligase"
                     /protein_id="HRV96957.1"
                     /translation="MEGAQRYFEKEWQRTVYCGDVSGKSGEELPVVVNGWVRRRRDLG
                     GLIFIELWDHTGAVQVVINPELTPEVHERARELRSEFVISVKGKIRVRPEGTANPSMR
                     TGEWEIVAGDLLRLAPAAPLPFEIGELTDKVDENLRLHNRYLDLRREKMQRNLRLRHK
                     IAMYTRNFLSDGGFLEIETPMLTLSTPEGARDYLVPSRVNPGSFYALPQSPQIFKQIL
                     MISGFDRYFQIVKCFRDEDLRADRQPEFTQIDIEMSYLVEEDIFSLIEKYLQGLFKET
                     IGVDVATPFRRMTYWEAMDRFGSDKPDLRIPFEIVDLSPVFAGSSFEPFREIAAQGGY
                     VRGIPLPGGASLSRKQLSDIEEKAKRLGARGLSPFQIKDGELKGPLVKYLDESSRSLL
                     KSSSSIDDGDALFVVAGSDRREISEILGALRLELAREHGLVAKGAWEFLWVVDFPLFE
                     RDEEAGRWSAVHHPFTSPCSEDIPLMDSNPGEVRSRAYDCVLNGNELGGGSIRIHDPL
                     LQEKVFSCLSFTKEAARERFGFLLDALSSGTPPHGGIALGMDRLAMLLSGAASIRDVM
                     AFPKT"
BASE COUNT         2984 a         3498 c         3844 g         3264 t
ORIGIN      
        1 gatacccgct gtcgccggct ttatcacctt ggcctgcgca gcgcttgccg ttcttgcggg
       61 tacgtcgggg gtaaaggctc tctgcggtct gtttctgtcg cttttcctgt tacttggatg
      121 gttcgttccg atgcttgccg aagggtatac gcctgtgcct ttcgctatct tggctatagc
      181 tggggtttct ctgctgacga tagtcttcgt cgttcccaaa cgcgctcttt ggccaattcc
      241 tttcatcggg gccgtaggag gagcaatcgg agcctcggtt acgggctgga ttatggttgg
      301 gctgtggcag ctgaccggac tggagaacga tagcgccgtg cttctttttt ccgttgcgcc
      361 tcaccttgaa ctgcgggggc ttcttcttgc cgcagtgatg gtcggcggca taggagctgt
      421 gcttgacgta gcggtttcgg tgacctcgtc gatggcggag ctgtactcct acgatccctc
      481 gatcccgctg cgaagactat ggagggccgg gatcaacgtc gggagcgatg ttctcggcag
      541 tatgatcaat acgttgatac ttgcctacct cgggagttcg ctcccgttcg tcgttctaat
      601 ctcgatggag ggcccagatt tggtcgcact tctcaacgat ccgcacatcg cacaggagat
      661 tttgcgcagt gttgccggca cggtaggact gcttctcaca attcctgtga ctgccggcgc
      721 cggcgtatgg tggatagcac gttctcatat cccggatgag aaaagacaca acgaagagct
      781 tgctgactga ccgttcactt tgtgataaga tatggcaatg tagattaaag ctattcatac
      841 acttctggag gtgttgcaat gaaaaaaata gcaatcaacg gacttggaag gatcgggcgg
      901 cttgttcttc gctgctatat ggcgaacaag cctgccgatg ttgagatcgt cgcattgaac
      961 gatctgacgc cggcctcgga gatggcctat ctgataaagt tcgactctat ccacagaaaa
     1021 gccaacttct cggtcgaagc tcgagagaac agcctggtac ttgacgggaa agaaatccct
     1081 ctttttaagg aaaaggatcc tgcaaaactt ccctggaaag agcttggtgt ggatattgtt
     1141 cttgagtgca ctgggttttt tacgaagaga gaaaaagcca tggcacacat cgaggccgga
     1201 gcgaagcggg tcatcatcag cgctcctgct gaagactccg accttaccat cgtcctcggc
     1261 gtgaacgaga atatgtacga cccggcaaaa catgtcgtcg tctccaacgc ctcttgcacg
     1321 acaaactctc tggcgccggt gacaaagatt cttaacgacg ctttcggcat cgaatatctg
     1381 atgggaacaa ccattcacgc ctacacatcg acacaggtcc tcgtcgatgt accgaagggg
     1441 ggcggcagaa aaggacgtgc cgctgcagtt tctcttgtgc ctgcaacaac gggtgcggca
     1501 aaggctatgg ttcctctttt ccccgagctt aaaggcaaga tggacatgat ctctgtacgt
     1561 gttccggtag ccgacggatc tctgacagat atcgtcgttc atttcaaaaa ggaagttacc
     1621 gtagagtccg tcaacgcggc gctgaaatcg gcagccgacg gaaaactgaa gggaatcgtc
     1681 gagtacaacg acgaagagat cgtctccgcc gatatcatcg gcaacacgca ctcgggtatt
     1741 gtcgacgcgc cttccaccaa ggtgatcatg ggcaaggtcg ccaaggtcat ggtgtggtac
     1801 gacaacgagt acggctattc gaacaggatg gtggagttgg cgcagtacat cgcgggcaag
     1861 gaataaccca tctttcacag ttcgttttgc aagcgcccga gtcccccgat gggagctcgg
     1921 gcgcttgcgt ttttacaaag attcagtgcg cgctgtccca gttgcttgta ctcttaagga
     1981 ttttccccac gctcaagaat catcttcaca aacgacttca ctatctttgc gtcataggct
     2041 ttccccgcaa gatcaataag ttccttcaga gcggctttca ccgtctttct ctttctgtag
     2101 gggcgatcgg atgtcatggc ctcaaaagca tctgcgacag acaagatacg cgcctcaatg
     2161 cgaatatctt ttctcttcag ggaatacggg tagcccgttc catcaacgcg ttcatggtgt
     2221 tgaagaacaa tctccgccaa tggccagggg aaattcagca gggagagaat atcgtgtccg
     2281 aattcggggt gtttttttac gagctcgtac tcggcttccg agagccggga gggctttccc
     2341 aggatttcgg caggtatata gagtttacct aaatcatgca cgagtcctgc gtacctgacc
     2401 cccatccgaa cctcttcggg gagctgcatt tcgtttgcaa ggccctctgc aagaacagaa
     2461 acatttttct gatgtcccga tgtggagacg tccctgagtt cgctgacctg gcagagcgct
     2521 gttatggctc cggtgaatct gtcgtgaatt cgctcatgcg tctcccgaag ggagtcctcc
     2581 tctttgcgtc gctcgatagc cattgccatc aggtcggaga aaagaccgag cagagggtca
     2641 ttggacatgt ctacccactg ctggagaggg tctgtgagcg ctagaaatcc ttggaaactt
     2701 gtcttgacga aaagagggag aatcgtgatg gagtcgatcc ccatttcctt tagaaaatcc
     2761 catttactct ctcttacttt atgtactgct gattcgatgg tgatgacgga gggaagggcg
     2821 tcgttcaata ggagagattt ccagaagaaa tcgtcgccgt cggagaagtt tgcggcaaga
     2881 gaggggcccg ctttgtccgg agaaaacgct tccatttcag gaatcagaat tcctgaatcg
     2941 gaaatcacga aaagcgaggc ggtacgcgct cccgaaagtg caagtatatc ttcgagcgcg
     3001 gcttccatgg agtccctgaa gttctggaag aggacgaatc gagaggaaat agctgtcatt
     3061 cgctcttgaa gaatcgttct ccgatggagc ttctgttcca tcgttttcct gtctcttata
     3121 tcatggaaga gagcgcaata atgagtttcc tcatcatttg acgacagggg aaaggcttgg
     3181 aatgagacag agagcgttcc gccgtccccg tcaggaagag ccgtctcctt tgccggcgcg
     3241 gctgtacctg aaatgttctc ctcctgtgga aaaatgtcgt ttcgcgaaaa atcagcttcg
     3301 gttccggaga gaagcgacgt gatattttct ggaagcggag ccggccccga aagacggaac
     3361 atttcccgga aacggctgtt tgcctctttt acttggcctg cgccatcgta gataagaata
     3421 ccttgaggag agtgttcaaa gagctttttc cagtactctg tctgtcgaag gagctcctct
     3481 tccgtttttt ttcgtctgtt gatctttctc ttcagataaa cggccaatcc cgcaaggaaa
     3541 agaaggagaa aaaggtttgc gaaaataaaa ggagtatact cctggatatt ataaaaggga
     3601 tctcctagaa taatacttcc cgggggaagg gccgatcttg ggatgctaaa ctcccgcatt
     3661 ccggagtagt taaaaagaag tttcggagcc tgtccttctc tcggaggcat cgattcggga
     3721 gacgatcctt ttaatagctc aaacgcaatt tctcccgcgt cgaatccctt ctgaaagcct
     3781 ccttcggaga cgctggccaa aacgcctaat gcggcccctt cttctatgag agagtacaca
     3841 gggagatccg aagcttcttg aatagcctct atcagacggg gtaaagaaac gagatctccc
     3901 tcccgggtaa tattgtagtg cccgaggatg ataacagagt tcggaggaag ctcggaaaga
     3961 gatgttgtga gctgttcgat atccattccg aaaagcggtt gcagacgttt tctcggaatc
     4021 gagagcattg ttgccgcctc gttcatttgt gcaatatcac gaaacccgcg aggcgacata
     4081 tccgtaacaa gcgcgatacg gggggcgttc tgatgaaggc ggattatttc ccgcaatata
     4141 aaaagcgagg tgttttcact gaagattccc gttccctttg tccgggggat aatagagaga
     4201 agcgctttgt tcgtaaggga gcagaagatg atgggaacgt cggggaaaag gacgcctctt
     4261 tgtccgagag cgaatgatgt agcgtcattt tccgttgtga tgataagatc cgggcgcatt
     4321 gaagtgtacc gagtacgata gagctcccgg agttccggga ggtgcattgc ggagaacgtc
     4381 cccgtgatat cgaggttttc aacaaagaat tcaacattgg ctccgttctc ctcaaaagct
     4441 gctgcgatgc ctcgatttgt ctcggcagtc cacgtgtctt caatggagta tgagttgagc
     4501 agtagaatct ttccggctac cctgggagcc gcgcacgatg aaagtgtgcc gtggagaaga
     4561 agcgctgcaa cgagtacgag ataccggaat tgctttcgta ggaaagaaat gttgatcggc
     4621 actatgtatc tcctctctcg ctttgtttag cgcggcaatg agaggtcagc cgcgctctct
     4681 cctgcctggt accctgtgct ccagcacatt tggaggttga acccgccggt aggaccgtcg
     4741 agatcgagca cttctccggc gaaaaagagg ttctccacca gcttggatcg catcgttcgg
     4801 ggctcgatct ccttcaacgc gacaccgccg ctggtcacta ttgcccagtc gaatccgaga
     4861 agtccgtccg gagtcagagg aaaggctttg agcagctcgg caagcgtttc gatttcctga
     4921 ttgcttgcgg agttcacaat tttatcctcc ggtagaggct tcagagcgag tactgcagga
     4981 atgagccccc ttgggagaag ctccccgaga gagtccttga attttctgcc ggagaacttc
     5041 tcaaaatccc tgccaatcct gtttttcagc acaggaagag aaagcgccgg cttgaggtcc
     5101 agagtaagcc tggccgtgcc cgtttccatg gcggctgcaa tctccttgct catgtccata
     5161 gcaatcggac cgctgatgcc gaaatgtgtc agcagaagct ctccaaagcg ttgcgatgcc
     5221 acttcatcat ttttccaaag agtgagtgag acgttacgta gtgtcatccc ccgggaagca
     5281 aggggccact tttccactgt tctgaccggg cagagcgccg gaacaggagg aatgacggtg
     5341 tgtcccgccc tgcgtgcgaa gcggtaaccg tcgcctgtcg atccagtctt cgcaaaggac
     5401 tttccccccg tacagatgat gaacgcgtct ccctccactt cttgtcccct gagtatgaag
     5461 cgacggaccc gaccgtcgcg aatttcgagg ttgatcgatt cacgcttgcg gtagatggtg
     5521 actccgcctt cccgtacata gttcagaagg gctgcgagca ccccctccga tccgcctgtt
     5581 gccgggaaga accgtttgcc ccgttcttcc ttgagcggca ctcccctgga ttggaaaaaa
     5641 gaaactgtct cgcggggtcc gaagccggcg aaagaggagt agagaaattt tccgttcggt
     5701 ccgtactttg agagaaagac atccatgtct tcctcggcgt ttgtcaggtt gcaccgtctt
     5761 ttcccgctga taaggagctt ttcaccgacg gtctgatttt tctcgaccag aatgaccctg
     5821 gcgcctttct cagccgcgcg tcccgcggca atcagtccgg cgggaccggc gccgattacc
     5881 acgatctggg gagcgctcat cggttcgtac cctttttctc ttcacgcatt gagagcgaga
     5941 tgcgtttgcg cggacgatcc acctcaagta ccgagacgtt gacgcgctct ccgggacgaa
     6001 actctttccc gtgcggcagg cgcttcctgt ggacgagtcc gtcctggtgt acgccgacgt
     6061 cgacaaagat accgaaagaa gtgatgtttg taacgatccc gggaagcggc atgccgggga
     6121 gcaggtcgtc aatcttctcg acgccctctt gaaatgtgaa cggctcgaac gtctcccgcg
     6181 ggtcgcgccc gggtttttcc agttcgttca gtatgtcggt aagcgtagga aggcccgcgt
     6241 cttcggagat gtaccgcttt acatcaattt tgttcttcat cgtcgggctt gagagcaggt
     6301 ccgggagaga acaccccaga tccgcggcta tacgctcgac aagcggatag cgttcggggt
     6361 ggactgcgct tgcgtcgaga ggattcgttc ctccgcggat tcgaaggaac cctgctgcct
     6421 gttcaaatgt tttcggtccg agccgcggga cgttcaataa ctcttttctg gaggagaacg
     6481 ggccgttcgc ctccctgaat ttaatgatac ggtccgctgt tccggagcct atgccggaga
     6541 cgaaagagag cagttcaagg ctcgccgtgt tcagttctac gccgactgcg ttcacgcagg
     6601 attcgacaac cgtcgcgagc gcatttttca gttctttctg atcaacgtcg tgttggtact
     6661 gaccgacgcc tatcgattta ggatcaagtt tgacaagttc ggcgagcggg tcgagcagtc
     6721 tgcgcccgat ggacactgcg ccgcgcaccg tgagatcatg ttccggaaac tctcgccgcg
     6781 ccgtctccga agcggaatag atcgacgcgc cgctctcgtt caccatcgtg atcagaatgt
     6841 ccgaagggag tccgagtccg cggaaaaaac gttctgtttc ccgccccgcc gttccgttgc
     6901 cgatggcgac ggcttcaatt ccatactcgt ctattgcctt ccgcatgatc tccgcgcttt
     6961 ttgcttcatc ggcacgggga ggatgaggaa aaattgtccc gttaaagagc agatcaccct
     7021 gccggttaag gcagacgaac ttgcagcctg tccggaaacc cggatcaact gcgagtatat
     7081 tccgggggcc gagcggcgag gcgagaagaa tctcccggac gttctttgca aagacagcga
     7141 tagcctccct gtccgccttt tttttcagcg cgttgcggag ttcggtctcc atggagggcg
     7201 cgagcaggcg ccgatagccg tcgataatcg cttcattcac aagtatggaa tcggggccta
     7261 cgccatgcac aaaaatatcc cggagaatac gaagcgcttc ttcctcgggt gggagcagag
     7321 agagcgaaag caccttctcc cgttctcccc tgaacattgc cagtatccta tgggaaggag
     7381 caagcgccgc gggctcttcc caagcgaacc agtccttgaa attggctcct tcttcttctt
     7441 ttcccttgat acagctgctt ttcagctttc ctctcctgcc gaaaagaatt cggacctggc
     7501 ggcgcgcctc cctattttcg ctgatacgtt cggcgataat atcctttgcg ccctgcagag
     7561 cctcttcttc tgttgcaact cctttttctt cggagaggaa agagattgca aggctgaaag
     7621 gatcgttccc ctcctgacgg agtagcaggt cggcgagtgg agcaagtcct ttctcagccg
     7681 ctatggacgc tctcgtcttg cgtttcggcc tgtatgggag atagatatct tcgagcgcag
     7741 taagagaagc tgcgttcgcg attgcctttt ccaattcagg attaagaagc ccacggtcag
     7801 taagcgaggc gtgaatcgcg cttctgcgcg cctccagctc ttcaagacgg atcgaacgct
     7861 ccttgatagc aaggacggcc tcttcatcca atgagcctgt cgcctctttc ctgtacctgg
     7921 caatgaacgg cacagtacag tctttttcga ggagcaggag cactgcgtgc acggagtcgc
     7981 gcggcagttt gagttctttt gacactcgtt cgcaaatttc ggtgttcatc aggaatcctc
     8041 cgatcccgca aatgcatgta tgaggcctct tgtcctctca ttctagcacg agaagaaatg
     8101 cggggaaata tcgtggtaga atctgttgtg aagagagggt tgctttgtgg gaagaaatac
     8161 gtggcttccg ctgtacagaa gacttgtttt ggggctgtct ctccccgttg acgcagaagc
     8221 gcttcgggga aaagtagcgc tgcacttctc ggatactccg tccacctttt acggagtgat
     8281 gaggcgtttg atacggtttt tcgagcctca gtgcatcgtc catacaggag acttggcgga
     8341 tgacgtgaag cttgagattc gtccgggcga actccctctg tatcgaagaa aactgatgga
     8401 gctcacttcg gtgctctcat ctgttcagaa gagcaggata gttcttgtta cggggaatca
     8461 cgatcacgaa gagagtgtcc gcgaattctt ccccgacagt tctgtaatta ttaagagcgg
     8521 gcgaatcgag atgtgcggtc tggatttcaa tctaagccac gaaccggaag gattgcagac
     8581 tccatacggc catttcaacc ttttcgggca tgcgcctgtt ccggaggaag cgccgagtca
     8641 aggacaggtg ttccttaatg gtctttatgc gattcatgtc ttacgtgtcg acacagggga
     8701 agtattggcc ctggagtacc ccgggtatgt gaacgacgcc cggctcaata aaagaagagt
     8761 cggcctttga ttacagtagg gaggtgtgtt gatgctttcg attgttcgag gcggtcctcg
     8821 ggttctcttc cttaagggag agcgaagcgc tttggatcat gcgctttccg ccgctttctc
     8881 cgtaacgagg catacctttg aaagcgccct ggactgcgcc ggagaggggc agacgattgt
     8941 cgcgcttcac ggaaaggagg atgacggagg gagagagttc cggattgttt cctcttcctc
     9001 tgacgaggtg cttgccgccc ttgtgaacga cagctcctgc cgccttgaat cagtccgtct
     9061 gcttcctcgt attctctttt tccgtatctt cggcgacaag gagcgtgtgc ttgatcagat
     9121 tgaaaacgat ctcgcagggg gaggtattcc cggagcaacc gtacggagag ggaggctgcg
     9181 agctctttta gggagttcac gaagaaacga gattgcggtc tgctttacgt acgctcctct
     9241 gaacggtctc atagccgtgg cggatatgct gaacgacgtg ctttttgtgg aagggacttt
     9301 cttttatgac ttaaatgtgt ttctccggag caggggcctc tggtatttca gcgaagggct
     9361 ggaaaaccgc tcatggaacg agatggaaat acggatatac gattcgtggg gttttttcat
     9421 ggagcatgcc gagcgtcttc gcgcactcct cggctctctg gagataggca tgatcctcgg
     9481 agaaggctgg gggaaggatt ttgcgcatat tctgatgcct gtccagatat accgccttcg
     9541 tctttttact tttctttcga cgctgctcgt caaggagttg ctcatggggc tggagtacgc
     9601 ggagaacggc gacaggctgg cggactttga tctgtatagc ggacgtgaca aaatctcatg
     9661 gggaagttta aaagaccgtg gagcaggacg cgcgaacctg ggaaaaacag cacgcgaaaa
     9721 gcttttttcc cggctttcag tgcgtgaccg cgaacggttg accgctatgg agcaccgtct
     9781 gcttcgcctg cggcagggtg gaaacaacgg gtgaaaatga gccgtcgcaa gacaggagtc
     9841 catctccttg cgcctcgaat tactgaaaca ggatgataga gcgttttttt gaaaaaagag
     9901 aaaaaagcga ttcactcctt gcaaagaact tgctcctgcg attatactaa tttagaaact
     9961 ttcggagaac tctagtggca gactcttagg ctttctcgcc ctgacgaatt gaaggagaac
    10021 ttgtttcttc gagctcatga ttcgccggat gcgttccgat atgcgcagat cgtcaatcta
    10081 cgaaggaggt cgctacttat ggaagtgctc aaggtctctg caaagtcaca gccaaagtcg
    10141 gtcgcgggag ccattgcagc ggtactgcgg gagaaagggg cggtcgaagt tcaggcagtg
    10201 ggcgccggag ccgtgaacca gtcggtgaag tccattgcaa tcgcaagggg gtatgtcgct
    10261 cccaacggta ttgatcttgt gtgtataccg gccttttcga aaatttccat tgacgatgaa
    10321 gaaaggacgg ccatcaagtt tcagctggaa tcgcgctgaa ttcacgcggc gtattcaacg
    10381 catttgagcc cggccccgaa ttgagggtcg ggttttgttt tttttagatc atctctggaa
    10441 aaaaaacgaa ggaatgggta tactcgcttg cataatgtat tcgccctcga ggtgtctcca
    10501 tcttttcttt gaggtgaagg aatgcaacag cgtttcggcg gggcaaaaac cgccggtcgg
    10561 gaggagaaaa catggcggaa atgagcgcac caaaaggagt tcgggatatt cttccggagg
    10621 aatcgtggaa atgggcgtac gtctttagga tggtgcagga gacggcccgt cattttggat
    10681 attcggaagt acatcttcct atttttgaac atacggagct gttttcaagg ggaatcggcg
    10741 atacaaccga cgttgtcgaa aaggagatgt acacttttct cgataaaggg gacaggagca
    10801 ttacgctccg tccggaggct acggcgtcca tggtgcgcgc atatcttgaa caccggatgg
    10861 gaacgatggc tcagccggtg aaaatgtggt gcgccggccc gatgttccgg tacgaacgcc
    10921 ctcaaaaggg acggtacaga cagttttggc aggtcgattt cgagagtatc ggcgcttctt
    10981 ctccctatgc cgatgtcgag attatcgcca tctccctgga actcttccgg cggctggggc
    11041 ttcgtcacct tgaggtggtg atcaattccg tcgggtgtcc cgagtgccgc cccgattacc
    11101 ggaagcgtct caccgactac ttcgagtcga gaaaggacaa tctctgcgag acgtgtctcg
    11161 ggcgcctcga aaggaacccc ctgcgcatat tggactgcaa gcgggacggg tgcaaagcgg
    11221 tcactgaaga ggctcccgat gtctacgaca gcctctgcgg cgaatgccgc gaacacttcg
    11281 acgtggtgaa agacagcctt gatgctctcg gcgcctccta ccgtcttgat aagcgccttg
    11341 tccgcggact ggactactat acgaagacgg cgtacgaaat tctctcagga gagctcggct
    11401 cccagaacgc tgtctgcggc ggaggccgtt acgacaacct cgcggaagcg atcggagggc
    11461 cgcatgtccc cggcgtcgga tttgccgcag gcgttgatcg tattgtgatg gtgatggagc
    11521 agcaggagtg ttcttttggt tccgttccca ccgtggaagt cttcgcggtt gccctggatg
    11581 atatctcgag gcggaatgtc ctgcagcttg tggatatgct tcgccggaac ggaatcgtag
    11641 cggagatgga tcttgccggg aagagtatga aagcgcagat gaaggccgct tcatcgcgcg
    11701 gcgcacgatt cgcctgcatt ctcggcgaga gcgaacgcac gcgcggcacg gtaatggtga
    11761 aggatctcgc aacggcggaa cagcaggaaa ttacactgtc cctgctcgtt gccgaactga
    11821 aaaataaact gacctctacc cgcaaagggt aaacggctcc tggagggaac gcttcatgga
    11881 aggtgcgcaa cggtatttcg aaaaagagtg gcaaagaacg gtctattgcg gtgatgtctc
    11941 cggaaagagc ggagaagagc ttcctgtcgt cgttaacggg tgggtcaggc gaaggagaga
    12001 tctcggcggt ctcatcttca ttgaactttg ggatcataca ggcgcagttc aggtggtaat
    12061 caaccccgag ctcacccctg aggtacatga gcgagcccgg gaactgcgca gcgaattcgt
    12121 tatttcggtg aaggggaaga ttcgcgtccg tcctgaaggg accgcgaatc cctcgatgag
    12181 gacaggagag tgggagatag tcgccggaga tttactccgc ctcgcccccg ccgctccgtt
    12241 acctttcgag ataggcgagt tgaccgacaa agtggatgag aacctgcgtc ttcacaacag
    12301 gtatctcgac cttcgcagag agaagatgca gcggaacctg cgtcttcggc ataagatagc
    12361 aatgtacacg cggaattttc tgtccgacgg cggatttctc gagatcgaaa ctcccatgct
    12421 taccctctcg actccggagg gagcccggga ttatcttgtg ccgagccgcg tcaacccggg
    12481 gtcgttttac gcgcttccgc agtcgccgca gatcttcaag caaatcttga tgatcagcgg
    12541 tttcgacagg tatttccaga tagtgaaatg cttccgtgac gaggatctcc gggcggaccg
    12601 gcagcctgag ttcacacaaa tcgacattga aatgagctac ctcgtggagg aggacatctt
    12661 ctctctcatc gagaagtacc ttcaggggct gttcaaagag acgatcggcg tggacgtcgc
    12721 gacgccgttc cgccgaatga cctactggga ggccatggac cgtttcggga gcgataagcc
    12781 ggatctccgc atcccgtttg aaattgtcga tctctccccg gtatttgcag ggagctcctt
    12841 tgaaccgttc cgcgaaatcg cggcgcaagg agggtatgtc cgcggtattc ctcttcccgg
    12901 aggggcctcg ctctcaagaa agcaactttc cgatatcgag gaaaaagcga agcgcctcgg
    12961 cgcccgcggt ctttctccct tccagatcaa agacggagag ctgaagggac ctttggtaaa
    13021 ataccttgac gagagctccc gatcgttgct caaatcatcc tcgtccattg acgacggcga
    13081 cgcgctcttt gtggtcgcgg ggagcgaccg tcgcgagatt tccgaaattc tcggggcgct
    13141 tcgccttgaa ctggctcgcg agcatgggct tgtcgcgaaa ggcgcctggg agtttctctg
    13201 ggttgtggat ttccccctgt tcgaacgcga cgaggaggcg ggacgctgga gcgcggtgca
    13261 tcaccccttc acctctccct gctctgaaga tattccgctc atggattcaa atcccggcga
    13321 ggtgcggtcc agggcgtacg actgcgtcct caacggcaac gagcttggcg gcggttccat
    13381 cagaatccat gacccgcttc tgcaggaaaa ggttttctcc tgtctctcct ttacaaaaga
    13441 agcggcgcga gagcgtttcg gattcctgct cgacgcgctt tcttccggca ctcctcccca
    13501 cggaggtatc gctcttggaa tggacaggct ggcgatgctc ctgagcggcg ctgcatccat
    13561 tcgcgacgtg atggcgttcc caaagacgca
//
LOCUS       DAOTIL010000003        11813 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminobacteriaceae bacterium isolate AMR_MDS_3026
            NODE_6458_length_11813_cov_4.110818, whole genome shotgun sequence.
ACCESSION   DAOTIL010000003 DAOTIL010000000
VERSION     DAOTIL010000003.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868050
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminobacteriaceae bacterium (wastewater metagenome)
  ORGANISM  Aminobacteriaceae bacterium
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae.
REFERENCE   1  (bases 1 to 11813)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11813)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:19:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,224
            CDSs (total)                      :: 3,198
            Genes (coding)                    :: 3,184
            CDSs (with protein)               :: 3,184
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11813
                     /organism="Aminobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6458_length_11813_cov_4.110818"
                     /isolate="AMR_MDS_3026"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:3034609"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..502)
                     /locus_tag="P5201_00070"
     CDS             complement(<1..502)
                     /locus_tag="P5201_00070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013047570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HRV96958.1"
                     /translation="MSGYAEGIIILLCINAIAAMGVSLLTGFTGIFSLGHAAYMALGA
                     YTSAILTVQHGVHFFPAILLGGVVAVALAYFIGVPTLRLMGDYYAIASIGLGEAIRLI
                     LENWQSVTRGARGYPGIDPYTSLPIAVGFFVILAIFMFNLINSRFGRACKACRDDYIA
                     SSLLGFH"
     gene            complement(516..1397)
                     /locus_tag="P5201_00075"
     CDS             complement(516..1397)
                     /locus_tag="P5201_00075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014162848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0015658 - branched-chain amino acid
                     transmembrane transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HRV96959.1"
                     /translation="MATLIQQIINGLSLGSVYALIAVGYSLVYSVLLFSNFAHGGFLV
                     IGGYICYAALRTFNIGIWMAGALSLVGAGAAAILTERLAYRPIRERTNVTLYLLIASM
                     GMSIVIENIFVIVAGGRFRALPPVIPTTPVNFFGLATTSAFDLLSLVSAVVFLGALQL
                     FLSKTRWGLAIRAAAYDLRTAGLMGVNVNRLISIVFFVAGALAAVGGIFLSVRYTLYP
                     QLGAITIKAFVAAVIGGLGSLPGAVVGSLILGLAEMLTAGFISSQMRDLVVFSLLVVT
                     LLVKPTGLFGKSVSEKV"
     gene            complement(1521..2792)
                     /locus_tag="P5201_00080"
     CDS             complement(1521..2792)
                     /locus_tag="P5201_00080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006301183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HRV96960.1"
                     /translation="MKRNWMIALVVLVVVAGYFFMQQQAPQPPKEEPVKEAPTPTPEA
                     PKAEEPKAEEPKAEEPKASEETVKIGYLAALTGDWAAYGQTEEKTARLAVDEINAKGG
                     VLGKKLELVVYDFRTRAEDAVNAVRRMIEEDKVVAIVGANGSGINIATAPIVNRLGVS
                     QIGTVSTNPLVTVDEKGEVRPYSFRICFTDPYQGKLIAFFAAKELKRMKAAILYDVGN
                     DYSQGLREFFMKSYAEYQGEVVADLGFRGGTDVDFRAQLTEIRDKGADVLVLPNMGKE
                     MALIMKQARELGMNEIVFVGGDGYGEFMWEIAGEAMEGSYWINHVAPEDPAMAPFFAA
                     YKEKWDDECKEFVNGVLGYDSIYWVADAISRAGKADPKLIRDALEATKDLKLHHATIT
                     MDPKTHNPVDKDGVVLIAKDGKGQFFTKIKP"
     gene            complement(3078..4307)
                     /gene="rocD"
                     /locus_tag="P5201_00085"
     CDS             complement(3078..4307)
                     /gene="rocD"
                     /locus_tag="P5201_00085"
                     /EC_number="2.6.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008710826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004587 - ornithine-oxo-acid transaminase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine--oxo-acid transaminase"
                     /protein_id="HRV96961.1"
                     /translation="MMSRNRSEELMEMEHEYGAHNYHPLEVVISKAEGIWVEDPEGRR
                     YMDMLSAYSAVNQGHRHPKIIKALKDQADILTLTSRAFFNDRWPVFAKKLADFTGKEM
                     ILPMNTGAEAVETALKTMRKWGYMVKGVEKDKAEIIVFENNFHGRTITIISFSIDPDA
                     TDYYGPFTPGFKVVPYDDLEAVEKAVTKNTVGIMVEPIQGEAGVVVPSDGFLKGLETI
                     ARKHNVLLAIDEIQTGFCRTGKNFAFQHEGIDPDIIIMGKALGGGVFPVSAIAANKDV
                     LGVFKPGTHGSTFGGNPLACAVASAAIDVLVDEKMVERAAEMGKYFMDGIKAIQSPKI
                     KLVRGKGLLIGVVLNESAGKARLYTTALKEKGLLCKETHDWIIRFAPPLVITKQEIDQ
                     ALKTIQEVFASTPIPCV"
     gene            complement(4592..6229)
                     /locus_tag="P5201_00090"
     CDS             complement(4592..6229)
                     /locus_tag="P5201_00090"
                     /inference="COORDINATES: protein motif:HMM:NF013696.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HRV96962.1"
                     /translation="MLSLAENDLYSLLLDLVAIPSVSFSEEENRAADFIFSTLSDLEY
                     FRRNPSFLRFLPAEGDPLGRKAVAALVKANPRTKKTVIITGHFDVVDAEACGHLKNLA
                     FSPEEYTRRVDELEIPEEARDDLASGEYLFGRRVSDMKSGIALEMCLIGALSRLESFP
                     ANVLFLAVPDEENTSAGMRGAVPWLVRLRDEWELEYAACLNSEPSVGGRGIPAGGVYV
                     GTIGKIMPFYLCVGKEAHVGDYYEGVSASLLTSYLSLILEGSPETAESLDGVHFPPQA
                     CLRFKDLVKNYSVTLPERSVAYYNCLTVSRTPARILEEMKTVAGRALEMTLTHLSKAR
                     QTLRERGASLPEKKAFPPRVLSYGDLLEKVRSRTERFDDVLESFLKTLPDSMDERDLC
                     IETASFLLDLSGEKGPLILVGFLPPFYPPRLNRSESAGERALLRAVERLKEAGKPYGL
                     SISRIDVFQGIMDLSYLGFQGAPSELDALAENMPLWGRGYRFPLNELKKLDVPIANFG
                     PIGKDDHKNAERIHLPYYLHTLPPLFFKFVEFLAEES"
     gene            complement(6236..8578)
                     /locus_tag="P5201_00095"
     CDS             complement(6236..8578)
                     /locus_tag="P5201_00095"
                     /inference="COORDINATES: protein motif:HMM:NF013117.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel"
                     /protein_id="HRV96963.1"
                     /translation="MKTTLRRCVVICAMLLAELFFCILIYGSASSANPPEASQEAEKT
                     VLEVAKVNVEASVREIPLDKQAIQDRITEIQTELDALIEKKESLLETENIEEDDLRRT
                     LVALGSLQNVYSRLTSAQDNLTEAKREQEAQEKKSAEPVISGQPPFNLSFYEDVRNRY
                     ESVEQRLEAVNSSLRLSEGSVSTVDEQIRRMEEQHRVLSEELQNLKNASGVKQLLQVK
                     ESEADLETAKASRIVQDLNIEAQNISRNLLLAERDKLKTGLEFVKKHVAYDEKDRTAR
                     AERIGEQIAAAREKLPGLREEREKARTALSRSQGALTSARNERERVLAQAGVAEQQAA
                     LQLAQVSLEHTELTLLFLEEEQRIWNARYSLLNGSLKGQELWDMRSLIVTRVKDLESA
                     FLVRQRDVSSIQAEMHAAQKELEEHTGANAVLARIRARIDLLNRIAESANASMARIFS
                     LFNQYNRLREEINDHIDAVKIAERVTTFGKERFLAFWNITLWSGDGFDITVAKLVFAV
                     SLFLAAFFLSGRLTDFLSRTLLNRFGLDSTGMMASRKILFFILMGALILAALDLVGIP
                     LTAFAFLGGALAIGIGFGAQNIFNNLISGFILMFSEPMRVDDTIEIDGLFATVQEIGT
                     RATRVKTFDNIDVLMPNSYFLNNKIVNWTLTDQKIRLRINVGVVYGSDARKVEELLLL
                     AAHDHTRVLRNPEPFVVFREFGPSALEFTLYFWIDMTNASTLKVSSDLRYRLIALFEK
                     NGIKIAFPQMDVHLDTLSPVDICLNRPRTADTEQTHTQEE"
     gene            complement(8672..9754)
                     /locus_tag="P5201_00100"
     CDS             complement(8672..9754)
                     /locus_tag="P5201_00100"
                     /EC_number="1.13.12.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006299584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004497 - monooxygenase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitronate monooxygenase family protein"
                     /protein_id="HRV96964.1"
                     /translation="MSIQLPVLKLGKHTPRYPLIQCGMGVAISGPSLAGHVAKCGGVG
                     TIASVGLAANSPHYNGRNYFDANQLAVKDAIADARKIAGPGGVIAVNCMVALTDYDRQ
                     TLSACQGGVDVIISGAGLPLRLPEYTRDFPDVALVPIVSSLKAAALITRRWEKTYGRV
                     PDGFVVETPKYAGGHLGVTKMDQVDDEQFTLEHVIPELVEYVEKEMGSDIPVIGAGGI
                     WGREDMEKVFALGAKGVQIGTRFACTFEGDASDRFKQAFIDATEEDVVIIHSPAGLPG
                     RALKSPFVAQYLEGNVKSKPCIANCLTHCRYKTEKETFCIAQALIDSFRGNWEEGLFF
                     CGSNVVKVKEMQHVEDIMRDFFPEMA"
     gene            9987..11285
                     /locus_tag="P5201_00105"
     CDS             9987..11285
                     /locus_tag="P5201_00105"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009200440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase, iron-sulfur-dependent,
                     subunit alpha"
                     /protein_id="HRV96965.1"
                     /translation="MFTVKEFLKSEVKPALGCTEPGAVALAVARAKEELPGDVESVVV
                     TVSDSIYKNGVDVGIPGTKGLRGNNVAAALAVLCGRSEYGLEVLKDCAEEDAAKALEL
                     LGKGCIKIIPDMERHGVFVEAKIQSGGKTALCRIEGSHTNIVEVLLDDKIRFSTVCPE
                     STNISEQAKSISEEVSRMGFSELVSMVDSMDEEDIDYVLKGVEMNMHIAEYGFSPDHD
                     SGLNLGKTIRKHSGTGFDSDDLSLKIKSYAAAAADARMSGAPLPVMSSAGSGNHGVTA
                     ILPVAIFAAHLGADKEETAKAVAFSHLATSYVKSRTGRLTPTCGCTVAAGAGAAAGMT
                     YLKTKDIARAAQAMLIILGNLVGMVCDGAKYTCALKVGTGALEAYHAALLVFDGHYPE
                     PQGLVDETIEKTVNNMVQVSVHGMQNLDKAIIDVISHRFN"
     gene            complement(11352..>11813)
                     /locus_tag="P5201_00110"
     CDS             complement(11352..>11813)
                     /locus_tag="P5201_00110"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRV96966.1"
                     /translation="KVATYGRWEDILLAARLHNKKELPAGISPAAMPLVQIVRDSDKL
                     DVFRLVRMHVEEDRINDLLPRIIPSGGYSEALVREVSSERRASYSNVRSLSDFLLVQL
                     SWVFDLNYAPSFRMLRRDGVLSWMERVLPQDASVNGFIQSALHHALLGESA"
BASE COUNT         2723 a         3336 c         3113 g         2641 t
ORIGIN      
        1 tgtggaaccc cagcagcgac gaggcgatgt agtcgtcccg gcaggctttg catgctctcc
       61 cgaaacggct gttgataagg ttgaacatga aaatggccag tatgacgaaa aacccgactg
      121 cgataggcag cgaggtgtac ggatcgatcc ccggataccc tcgggctccc cgcgtcacag
      181 actgccagtt ttcaaggata agtctgatgg cctcaccaag gccgatggag gctatagcgt
      241 agtagtcgcc catgagtctg agcgtcggaa cgccgataaa gtaggcaagg gcgacagcca
      301 ccactccgcc gagaagaatc gcggggaaga aatggacgcc gtgctgtact gtaagaatcg
      361 ccgacgtata ggctccgagc gccatatacg cagcatgccc caaagagaat atgcccgtga
      421 atcccgtgag gagagagact cccatggctg cgattgcgtt gatgcacaaa agaatgataa
      481 taccttccgc ataacctgac atggtcttcg ctcacctaca ctttctcgct gacgctcttg
      541 ccgaaaagcc cggtaggctt gacaagcaac gtgacgacca gcagggaaaa gacgacgagg
      601 tcgcgcatct ggctggatat gaaaccggcc gtgagcatct ccgcaagccc gaggatgagg
      661 ctgcccacca cggcgccggg aagggaacca agaccgccga taacagccgc tacaaatgcc
      721 ttgattgtga ttgcgcccag ctgggggtac agtgtgtagc ggacggagag gaaaattcct
      781 cccacggcgg cgagcgctcc cgcgacgaag aagacgatgg agatcaagcg attgacgttc
      841 actcccatga ggccggcagt tcgaagatca tacgcagcag cccttatggc aagcccccat
      901 cgggtcttcg acaggaagag ctgcaatgcg ccgaggaaaa caacagccga aacaagggaa
      961 agaagatcga acgcgcttgt agtggcaaga ccgaaaaagt tcaccggcgt ggtcgggatc
     1021 accggaggaa gagcgcggaa acgtcctccc gccacaatga caaagatatt ttcgatgaca
     1081 atgctcattc ccatcgaggc aataagaaga taaagagtca cattggtgcg ttcgcggatc
     1141 gggcgatagg cgagccgttc cgtgagaatg gccgccgctc ccgctcctac cagagaaagc
     1201 gcacccgcca tccatattcc gatgttaaaa gtacgaagcg cagcgtagca gatataaccg
     1261 ccgattacaa gaaagcctcc gtgggcgaag ttggagaaga ggagaacgga atagaccaga
     1321 gagtatccca cggcaatgag tgcgtagacc gatccaagcg agaggccgtt gatgatctgc
     1381 tgtatcagcg ttgccaaata tggatcaccc ctttcacgta ttgacgaggg cagacggtct
     1441 ccgcctaccc tcgtcatgga acaactatat catatttttt tcttcacggt atattgtatt
     1501 atttaaactc tgcggtgcac ttacggcttg atcttcgtga agaactgtcc cttgccgtcc
     1561 ttggctatga gaacgacgcc gtccttgtcg acagggttgt gcgtcttcgg gtccatggtg
     1621 atggtggcat ggtgaagctt cagatctttg gtggcctcaa gcgcgtcgcg gatgagcttc
     1681 ggatcggcct taccggcgcg ggagatcgcg tcggccaccc agtaaataga gtcatagccg
     1741 agcacaccat tcacaaactc tttgcactcg tcatcccact tttccttgta cgccgcgaag
     1801 aagggcgcca tcgcaggatc ctcgggagcg acatggttaa tccagtagga gccctccatg
     1861 gcctctcctg cgatctccca catgaactcg ccgtacccgt cgccgccgac gaagacgatt
     1921 tcgttcatac caagctcgcg tgcctgcttc atgatgagcg ccatctcctt gcccatgttg
     1981 ggaaggacga gcacatcggc gcccttgtcg cggatctcgg tgagctgcgc gcggaagtcg
     2041 acatccgtgc cgccgcggaa tccgaggtcg gcgacaacct cgccctggta ctcggcgtag
     2101 ctcttcatga agaactcacg aaggccctga gaatagtcgt tccccacgtc atacagaata
     2161 gcggccttca ttctcttaag ttctttcgcc gcaaagaaag cgatcagctt tccctggtag
     2221 gggtcggtga agcatatacg gaatgagtac ggacgaacct cgcccttctc atcgacggtg
     2281 acgagaggat tcgttgaaac ggtgccgatc tgggatacgc cgagacggtt cacaatgggc
     2341 gcggtagcga tgttgatgcc gctgccgttg gctccgacga tagccacaac cttgtcttct
     2401 tcgatcatac gccggacggc attcacagca tcttcggcgc gcgtacggaa gtcatagaca
     2461 acgagctcca acttctttcc aaggacgccg ccttttgcgt tgatttcatc aactgcaaga
     2521 cgggccgttt tctcctcggt ctgaccgtag gccgcccagt cgcctgtaag agcggcaaga
     2581 tagccgatct tcacagtctc ttcagacgct ttcggttctt ctgctttcgg ctcctcagcc
     2641 tttggctctt cagcctttgg cgcctcgggc gtcggcgtgg gagcttcctt caccggctcc
     2701 tccttcgggg gctgcggcgc ctgctgttgc ataaagaaat acccggcgac gacaacaagc
     2761 acaacgagag caatcatcca attccgtttc aaaaccaacc acctcctgag aattttgtgt
     2821 tccaccgtac ggtttcactg cttttcagaa gtcattttac tacgtcgcgc tctatctctc
     2881 aaatattttc ggctcttttg gggctcttca agaaagaagt tttctgtaga gacaatttct
     2941 gttttggagc ttctttgacg tccgtcattt tttcatacat aaaaggaaca cgcgattcac
     3001 tgcaagcgag ttgcatccca taaagtttgc ggcagcctct tcaggaagct gccgcaaacg
     3061 catcggggcg gattctttta cacgcaggga atcggcgtcg aagcaaacac ttcctgaata
     3121 gtcttcagcg cctggtcgat ctcttgcttt gtgatgacaa gaggcggagc aaaacggatg
     3181 atccagtcgt gcgtctcctt acagaggagg cccttctctt tcagcgccgt cgtatagaga
     3241 cgggccttcc ctgccgactc gttgaggaca acgccgatga gcaggccttt acctcgcaca
     3301 agcttgatct tcggcgattg aatcgccttg ataccgtcca tgaagtattt tcccatctcg
     3361 gccgcgcgct ctaccatctt ttcgtctacg agcacgtcga tagcagcgct ggcaacggca
     3421 caagccaacg gattgccccc gaaggtcgaa ccgtgggttc ccggcttgaa cacacccagt
     3481 acatccttgt tcgccgctat ggcggaaacc ggaaagactc cgccgccaag cgcctttccc
     3541 atgatgatga tgtcgggatc gattccctcg tgttggaacg caaaattctt ccctgtgcgg
     3601 cagaaaccgg tctgaatctc gtcgatggcc agaagcacat tgtgcttcct ggcgatcgtc
     3661 tccagcccct tcaggaaacc gtcgctcgga acgacgacgc cggcctcacc ctggatgggc
     3721 tcgaccataa tgccgactgt atttttcgtc accgcttttt cgaccgcttc gaggtcgtcg
     3781 taaggaacta ctttgaatcc gggggtgaac gggccgtaat agtccgtggc gtcgggatct
     3841 atcgagaagg aaataatggt aatggttcgt ccgtgaaagt tgttttcaaa gacgatgatc
     3901 tccgccttgt ccttctcaac tcccttgacc atgtaccccc attttctcat agtcttcaaa
     3961 gccgtttcca ccgcctcggc tccggtattc atggggagta tcatctcttt tcccgtgaaa
     4021 tcggcaagtt tcttcgcgaa gaccggccaa cggtcgttga agaaggcgcg ggaagtcaag
     4081 gtgaggatat cagcctgatc tttcaaagct ttgatgatct ttggatgtct gtgcccctgg
     4141 ttgactgcgg agtatgcgga taacatatcc atgtaccgcc gcccctccgg gtcctcgacc
     4201 caaatacctt cggctttcga gatcacgact tcaagcggat gatagttgtg ggcgccatat
     4261 tcgtgttcca tctccataag ctcttccgat ctgtttcgag acatcatgcc acctctttct
     4321 gaaggtatag taaatttcgt ttcactattt ctaaggcgtg cgaaacgatg tttacaaaaa
     4381 gtagtgcatc cactattgct attccagaat tgattctttc attgaatcat tatccaccat
     4441 aatcctcttt ttctcaactc cggaaaaaca aaaatataaa ccctcagaag aggagaaggt
     4501 ttcctgtata tactttgaaa tgcaaaagcg ggttttcgtc tccgaaaacc cgcttttata
     4561 atcagtattg atcagtagct ccaccctctt tttaagattc ttcagcgaga aactccacga
     4621 atttgaaaaa aagcggggga agcgtgtgaa ggtagtacgg caggtgaata cgctcggcgt
     4681 tcttgtggtc atcctttccg atcgggccga agtttgctat cggaacgtcg agttttttca
     4741 gttcgttaag agggaaacgg taccctctcc cccagagagg catattctcc gccagggcgt
     4801 caagctccga cggcgctcct tgaaacccga ggtagctcaa gtccataatt ccttgaaaaa
     4861 catcgattct acttatggaa aggccatacg gctttccagc ctccttgaga cgttccaccg
     4921 cccgcagcag agcgcgttct ccggcgctct cgctcctgtt gaggcgcgga gggtagaacg
     4981 gcggtaaaaa tccgacgaga atgagcggcc ccttctcccc ggaaagatcg agaagaaagg
     5041 acgccgtttc gatacagaga tcgcgctcgt ccatggagtc aggaagcgtc ttcagaaaag
     5101 attcaagcac gtcgtcgaat cgctccgtcc ggctacggac tttctccaga agatctccgt
     5161 acgaaaggac gcgcggaggg aacgccttct tctcggggag cgaagcccct cgttcgcgca
     5221 gcgtctgcct ggctttcgac aggtgggtaa gcgtcatctc aagagcccgt ccggcgaccg
     5281 ttttcatttc ctcaagaatc cgggcgggcg tccgggaaac ggtgaggcag ttgtaatacg
     5341 ccacggagcg ttcggggagc gtcacagaat agttttttac cagatccttg aagcgaaggc
     5401 aggcctgcgg cggaaaatga accccgtcca gggattccgc agtctccggg cttccctcaa
     5461 ggatgagcga aagatacgac gttaaaagcg acgcgctgac cccctcatag tagtcgccga
     5521 cgtgcgcctc cttccccacg cagaggtaga aaggcatgat ctttcctatt gtaccgacat
     5581 agacgcctcc ggcaggaatg ccccgcccgc cgaccgacgg ttcactgtta aggcacgctg
     5641 cgtattcgag ctcccattca tcacgaagac gtaccagcca gggaacagcc cctcgcattc
     5701 ctgcggaggt attctcctcg tcgggaacag cgagaaacaa aacgtttgca gggaaactct
     5761 ccaaacgtga gagcgctcct ataaggcaca tctcaagcgc tattcctgat ttcatgtcgg
     5821 aaactctgcg cccgaagaga tactctccgc tcgccagatc gtcccgagcc tcctccggta
     5881 tctccagttc gtccacacgg cgggtatact cttcggggga gaatgccaaa tttttgaggt
     5941 gaccgcacgc ttccgcatcc accacgtcga aatgtcccgt aataatgacg gtctttttcg
     6001 tccgggggtt cgccttcacg agcgcagcga cggccttgcg tccgagcggg tctccctctg
     6061 ctgggaggaa acgcagaaag gaggggtttc gccgaaagta ctcaagatcc gagagcgtag
     6121 aaaaaataaa atccgcagcc cggttctcct cctcggagaa ggagacgctg ggtatggcga
     6181 caagatcaag aagcagcgag tacaggtcgt tttctgcaag cgagagcatg aaatgctatt
     6241 cctcctgtgt atgtgtttgt tcagtatcgg cagtacgcgg acggttcaga caaatatcga
     6301 caggcgagag agtatccaga tgcacatcca tctgcggaaa ggcgatcttg atcccgtttt
     6361 tttcaaagag ggcgatgagg cgatagcgca gatcgctcga cactttgagc gtcgaagcgt
     6421 tcgtcatatc gatccagaaa taaagtgtga attcgagcgc gctcgggccg aactcccgga
     6481 agacaacgaa gggctccgga ttacgcagta cacgcgtatg atcgtgggct gcaagcagaa
     6541 gaagctcctc cacttttcgg gcgtcggaac cgtacacaac gcctacgttg attctcaggc
     6601 gaatcttctg gtcagtgagg gtccagttga caattttatt attaagaaaa tagctattgg
     6661 gcatgagcac atcgatgttg tcgaacgttt ttactcgcgt tgcgcgggtg cctatctcct
     6721 gcaccgtcgc gaaaagaccg tctatctcga tcgtatcgtc cactcgcatc ggttcgctga
     6781 acataagaat aaaaccactg atcaggttgt tgaaaatatt ctgggctccg aaaccgatgc
     6841 cgatggcaag ggcgcctccg aggaacgcga acgccgtaag cggtatgccg acaagatcga
     6901 gcgcggccag aataagggcg cccatcagga tgaaaaagag aattttcctg ctggccatca
     6961 tcccggttga atccagaccg aaacggttaa gcaacgtcct gctgaggaaa tcggtcagac
     7021 gtccgctcag aaagaacgca gcgaggaaga gggagaccgc gaaaaccagt tttgccactg
     7081 tgatgtcgaa cccgtctccg gaccagagag tgatgttcca aaacgccaaa aaacgctcct
     7141 tgccgaaggt ggtaactcgt tccgcaatct tcaccgcgtc gatatgatcg ttgatctcct
     7201 cacggagtcg gttgtactga ttaaaaagcg aaaaaatccg ggccatcgag gcgttcgcgc
     7261 tctcggctat gcggttgagc agatcaatcc tcgcacgtat tcttgcaaga acggcatttg
     7321 ctccagtgtg ttcctccagt tctttttgcg ccgcgtgcat ttccgcctga atggaggaaa
     7381 catccctttg acgaactagg aaagcgctct ccaaatcctt cacgcgtgtg actatgagcg
     7441 agcgcatatc ccaaagttcc tgccccttga gacttccgtt gaggagagaa tacctggcat
     7501 tccatatccg ctgctcctct tcgagaaaga gcagcgtcag ttccgtatgt tccagagaaa
     7561 cctgcgcgag ttgaagcgca gcctgctgct cggctactcc agcctgcgcg agcacccttt
     7621 ccctctcgtt tcttgctgaa gtcagcgctc cctggctcct tgagagggcg gttctcgcct
     7681 tctctctctc ttcgcgcagc ccggggagct tctctctggc cgccgctatc tgctcgccga
     7741 tgcgctcggc ccgcgccgtc cggtctttct cgtcgtacgc tacatgtttc ttaacgaact
     7801 ccaatcctgt tttcagtttg tcgcgttcag cgagaagcag attgcggcta atgttttgcg
     7861 cttcaatgtt caaatcctgg actatccggg acgccttagc ggtctcaaga tcagcctcgg
     7921 attcctttac ctggagcaat tgcttcacgc cggaagcatt tttcaggttc tgcaattctt
     7981 cggaaaggac tctgtgctgt tcttccatcc tccgtatctg ctcatctact gttgaaacgc
     8041 ttccttcgga aagacggaga gaagaattca ctgcttcaag acgctgctca acagactcgt
     8101 accggttacg gacgtcttcg tagaatgaga ggttgaaggg cggttgtccg ctaatgacgg
     8161 gctccgctga cttcttctcc tgcgcctctt gctcccgctt cgcttccgta agattatcct
     8221 gggcgctcgt cagcctggaa tagacgtttt gaagagagcc gagagcgaca agcgtccttc
     8281 gcagatcatc ctcttcaata ttctcagtct ccaagaggct ctctttcttc tcaatgagag
     8341 cgtccaactc cgtctgaatt tcagtgatgc gatcctgtat agcctgcttg tccaatggaa
     8401 tttcacgaac ggaggcctcc acgttgacct tcgcaacctc cagaaccgtt ttttccgctt
     8461 cctgcgatgc ttcgggaggg ttcgcagagc tcgcgctccc ataaatcagg atgcagaaaa
     8521 acagttctgc caagagcatt gcgcagatca caacgcaacg acgcagggta gtcttcatcg
     8581 tgttctcctc cctctttcta tcagaaaagg cgggagcaag ctcccgcctt gaaaaggaaa
     8641 agcgcggcag aaacgctccg gcgagtccga attatgccat ctcgggaaag aagtctctca
     8701 ttatgtcttc gacgtgctgc atttccttta ccttcacgac gtttgatccg cagaagaaga
     8761 gcccttcctc ccagttgccg cggaaggagt cgatcaaggc ctgcgcgatg cagaaagttt
     8821 ctttctccgt cttgtacctg cagtgcgtca gacagttcgc aatgcacggt ttgctcttaa
     8881 cgttcccttc caggtactga gcgacgaagg gagatttgag cgcacgcccg ggaagaccgg
     8941 cggggctatg aatgataaca acgtcctctt cggtcgcatc gatgaacgcc tgcttaaacc
     9001 tgtccgaagc atccccctcg aacgtacagg cgaaccgtgt cccgatctgc actcctttag
     9061 ctccgagggc gaagaccttt tccatatctt cgcggcccca tatgccgccc gcgccgatga
     9121 ccgggatatc gctacccatc tctttttcga cgtattcgac gagttcgggg atcacatgtt
     9181 cgagggtaaa ctgctcgtcg tccacctggt ccatcttcgt cactccgaga tgtcctccgg
     9241 cgtacttcgg cgtctcgacg acaaaaccgt cgggaacacg cccgtacgtc ttctcccatc
     9301 tgcgggtgat cagcgcggcg gctttcaacg agctgacgat cggaacgagc gccacatccg
     9361 ggaagtcgcg agtgtactcg ggcaggcgga gaggaagccc cgctccggag atgatgacgt
     9421 caacgccccc ctggcaggcg gaaagagtct gccggtcgta gtccgtaagc gccaccatgc
     9481 agttgacggc gatcactccg ccgggaccgg cgattttcct cgcatccgct atggcgtctt
     9541 tgactgccag ctggttcgcg tcaaaatagt tgcgaccgtt gtagtgtggg gagtttgccg
     9601 caagtcctac gctggcgatc gttcccaccc cgccgcattt cgccacatgc cccgcgagac
     9661 tgggtcctga aatcgcaact cccatgccgc actgtatcaa gggataccgg ggcgtatgtt
     9721 ttcctaactt taaaacgggc agttgtatac tcaaaaagat tctctcctct gcaaagtgtt
     9781 ctctggatct tgcccttcaa acaaagggct atcatattct tctattgtat cttatagtga
     9841 acaacacgag caagaaaaaa atatagcttc tttcccgtgt ttcgcactgt ccccaccaag
     9901 aaacgattga cgtgcttttc gttgcacggt accatttgtc tagtatatac cctgattctc
     9961 aaattttact gcgtggaggg gacaaaatgt tcaccgtcaa ggaatttttg aagtccgaag
    10021 taaaacccgc cctcgggtgt accgagcccg gagcggtggc attggctgtc gcccgggcga
    10081 aagaagagct gccgggagat gtggaaagcg ttgtcgtgac tgtaagcgac agcatctaca
    10141 agaacggcgt ggacgtgggg attccgggca ctaagggtct tcgcgggaac aatgttgccg
    10201 cggcgctcgc cgtcctctgc ggacgatcgg agtacggcct cgaagttctg aaggactgcg
    10261 cagaagagga cgcagcaaag gctttggagc tgctgggcaa ggggtgtatc aagataatcc
    10321 ccgacatgga gcggcacgga gtctttgtcg aagcaaaaat ccaatcgggc gggaaaaccg
    10381 ctctctgccg catcgaagga agccacacca acatcgtgga agttctgctc gacgacaaaa
    10441 tccgcttctc cacagtatgc cccgaatcca caaacatttc ggaacaggca aagagcatct
    10501 ccgaggaagt gtcgcggatg ggattctcgg agctcgtgtc gatggtggac tccatggacg
    10561 aagaggatat cgactatgtc ctcaaaggcg tggagatgaa tatgcacatc gcggaatacg
    10621 gtttctcccc cgaccacgat tcgggactca atctaggtaa aaccattcga aagcactccg
    10681 gaacaggatt cgattccgac gacctcagcc tgaagataaa atcctacgcc gcggctgcgg
    10741 cggacgcccg catgtcggga gctcccctcc ccgttatgag cagcgcaggc agcggcaacc
    10801 acggagtcac cgccatcctt ccggtggcga tcttcgccgc gcacctgggc gcggacaagg
    10861 aagaaaccgc gaaagctgtc gcattcagcc acctcgccac aagctatgta aaaagccgca
    10921 ccggacgcct gacgccgacc tgcggctgca ctgtcgccgc cggcgccggc gctgcggcgg
    10981 ggatgaccta tttgaagaca aaagatatcg ccagggccgc tcaggccatg ctgatcatcc
    11041 tcggcaacct cgtcggcatg gtctgcgacg gagcgaaata cacctgcgca ctgaaagtag
    11101 gaaccggcgc gctggaggcg tatcacgcag cgctgcttgt cttcgatgga cactaccccg
    11161 agccccaggg gcttgtagac gaaaccatcg aaaaaaccgt gaacaacatg gtccaggttt
    11221 ccgtccacgg catgcagaat ctcgataaag cgatcatcga cgtcatctca caccgcttca
    11281 attaacggac gtcacgaagc ggcccggaag atcgctcgct ctccgggcgc ttcgttggct
    11341 ctttttctgc cttacgcgct ctccccgagt aaagcatggt gaagagccga ctgaataaaa
    11401 ccgttcacag acgcatcctg cggcagaacg cgttccatcc acgagagaac cccgtctctc
    11461 cgcagcatac ggaaggacgg agcataatta aggtcgaaca cccaggagag ctgtaccaga
    11521 agaaaatcag agagggaacg gacattgctg taggaggctc tcctttcaga ggatacttca
    11581 cgtaccagcg cctccgaata gcctcccgag ggaataatcc tgggcaacag atcgttgatt
    11641 ctgtcctcct ccacatgcat ccggacaaga cgaaagacgt cgagtttgtc gctgtcgcgg
    11701 acaatctgga ctaaaggcat cgccgcaggt gaaattcctg cgggaagctc ctttttatta
    11761 tggagccgcg cggcgagcag gatatcctcc catcgcccgt acgttgcgac ctt
//
LOCUS       DAOTIL010000004        11334 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminobacteriaceae bacterium isolate AMR_MDS_3026
            NODE_6815_length_11334_cov_4.105594, whole genome shotgun sequence.
ACCESSION   DAOTIL010000004 DAOTIL010000000
VERSION     DAOTIL010000004.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868050
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminobacteriaceae bacterium (wastewater metagenome)
  ORGANISM  Aminobacteriaceae bacterium
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae.
REFERENCE   1  (bases 1 to 11334)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11334)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:19:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,224
            CDSs (total)                      :: 3,198
            Genes (coding)                    :: 3,184
            CDSs (with protein)               :: 3,184
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11334
                     /organism="Aminobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6815_length_11334_cov_4.105594"
                     /isolate="AMR_MDS_3026"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:3034609"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..216
                     /locus_tag="P5201_00115"
     CDS             <1..216
                     /locus_tag="P5201_00115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008521427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase Der"
                     /protein_id="HRV96967.1"
                     /translation="FDTLPTDQKGRPFKIYYCTQSGVEPPTFIFFVNHPDLASPAFEN
                     HMEKALRGLEDFSGTPIRIFWRGKEEK"
     gene            346..630
                     /locus_tag="P5201_00120"
     CDS             346..630
                     /locus_tag="P5201_00120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013048466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0030261 - chromosome condensation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HU family DNA-binding protein"
                     /protein_id="HRV96968.1"
                     /translation="MYTVTKAELITEVAKATGLNKKESGAAVGAVFEAIEAALEKGEK
                     IQLVGFGTFEVRERAAREGRNPQDPKKVIKIPAKKIPVFRPGKALKDKVE"
     gene            complement(663..1499)
                     /gene="rsmA"
                     /locus_tag="P5201_00125"
     CDS             complement(663..1499)
                     /gene="rsmA"
                     /locus_tag="P5201_00125"
                     /EC_number="2.1.1.182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008523015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000179 - rRNA
                     (adenine-N6,N6-)-dimethyltransferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (adenine(1518)-N(6)/adenine(1519)-N(6))-
                     dimethyltransferase RsmA"
                     /protein_id="HRV96969.1"
                     /translation="MGREQKGFKNKKSLGQNFLVDRNILRHILEQADLSVDDTVLEIG
                     AGQGVLTRELAASPCSFVFSVEIDEGLSEYLRDITLLYPNTFSLLWGDALAVDYSSLS
                     PVPTKVVANIPYNITTPLLWRLLEQLPEAGTGYFLLMVQKEAAERITAPPGTKERYPL
                     GITLELMGKARSVRTVHPQSFRPVPAVESSLLEIRLSGMHATLPKDSLWRNLLRSSFA
                     QRRKKLSNNLRGFYPSVSWSEAFERTEIHENARAEELTGEQWLALWFFLKTFEKKSLS
                     PE"
     gene            complement(1562..1639)
                     /locus_tag="P5201_00130"
     tRNA            complement(1562..1639)
                     /locus_tag="P5201_00130"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1602..1604),aa:Pro,seq:ggg)
     gene            complement(1798..5355)
                     /gene="nifJ"
                     /locus_tag="P5201_00135"
     CDS             complement(1798..5355)
                     /gene="nifJ"
                     /locus_tag="P5201_00135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009166018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016903 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors [Evidence IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate:ferredoxin (flavodoxin) oxidoreductase"
                     /protein_id="HRV96970.1"
                     /translation="MKRNWKTMDGNTAAAHVSYAFTEVAAIYPITPSSNMPEVVDEWA
                     AHGRKNIFGKTVRVTELQSEAGAAGAVHGSLSAGSLSTTFTASQGLLLMMPNMYKIAG
                     ELLPGVFDVTARSVAAHALSIFGDHSDVMACRGTGFAFLASGSVQEVMDLTAVSHLSA
                     IKGSLPFVNFFDGFRTSHEIQKIDVLEYDDLAKLVDYDALAKFKARAMNPEHPYQKGT
                     AQNPDIFFQAKEAVNRFYTVIPDIVESYMQEIKKLTGREYHPFNYYGDPNAENIIIAM
                     GSVCQAIEETVDYLNARGEKTGVVEVHLYRPFSEKYFFDVLPASVRRIAVLDRCKEPG
                     ALGEPLFEDICAIFKDKAEKPIVVGGRYGLGSKDTTPTHIKSVFDNLKLYQPRDRFTI
                     SIVDDVTGTSLPVNEQIVAAPEGTIRCKFWGLGSDGTVGANKNSIKIIGDETDLYAQG
                     YFAYDSKKSGGITVSHLRFGKKPIKSTYLIDTADFIACHKQEYVNLYDVLGGLKKGGS
                     FLLNTQWDTIEKLEKHLPSRMRRALANNDIQFYILNGTDIGEELGLGNRINMIMQAAF
                     FKLTNVIPINDAVKYMKDAIDHTYSRKGEAILEMNYKAVDAGINAIKKIDVPAEWANA
                     TSEPVLLSGLPSDIPDFVKDVCMPINAQQGDSLPVSAFLGREDGKFPSGTAAFEKRGV
                     AVNVPEWKSDKCIQCNQCSMVCPHAAIRPFILNEQELAAAPQGFGAVEVKGKEFPGCK
                     YKMQVDTLDCMGCGNCADICPVGALEMKPIVSQEKEIPNWLYAVEEVSDKSAETNKFT
                     VKGSQFSQPLLEFSGACAGCGETPYIKLVTQLFGDRMIVANATGCSSIWGGSAPNMPY
                     TKNSKGQGPAWANSLFEDNAEYGYGMKLSVNATMQYVIDAMNGLLALDIPEDVKAVVS
                     EWLESKDDGDKSKAAAEKVLAILDRDIGEEGNKLLCEIACHKDYLVKKSVWIFGGDGW
                     AYDIGFGGLDHVLASGEDINVFVLDTEVYSNTGGQSSKSTQTAAIAKFAASGKRVKKK
                     DLGRIAMTYGYVYVAQVAMGADKNQLMKAIKEAEAYNGPSLVIAYAPCIAHGISAGMG
                     KTQDQTKKAVEAGYWHLYRYNPELAEAGSNPFVLDSKEPKADFKDFLMSEVRYASLKK
                     EFPDTADELFEKAAKDARERYEIYKQLAEMGKQPVKAEA"
     gene            5757..6764
                     /gene="trmB"
                     /locus_tag="P5201_00140"
     CDS             5757..6764
                     /gene="trmB"
                     /locus_tag="P5201_00140"
                     /EC_number="2.1.1.33"
                     /inference="COORDINATES: protein motif:HMM:TIGR00091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008176 - tRNA
                     (guanine-N7-)-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(46)-N7)-methyltransferase TrmB"
                     /protein_id="HRV96971.1"
                     /translation="MIDIREIIIMPEKQQLPLNILRARGETRVFLEIGFGNGEFLVHL
                     ARQNPDAVFWGVEMSRSCVLRALKRIRREELRNIFLLCGDARFILKECLPKESLDGIY
                     MNFPCPWPKKKHTKRRVSSGDFPSEIASVLKKGAFFELVTDEEWYGMEVRNVLAAHPL
                     LELTEWKINPPRPVTTKYERKWMEMGKRIFLSRLQKKEAESSVGYRCLGRTEDVHIRV
                     PGKGSLKKFVNDIHNMGGEKGDALWVYKRYYESAEGNAYIIETVAGDGSFEQKFFIQI
                     VEREEDALIKIAPYSSPFLTDSVRGALEGMAHLIETHYLSIASSCAASLEQPAGKAFS
                     Q"
     gene            6761..7321
                     /locus_tag="P5201_00145"
     CDS             6761..7321
                     /locus_tag="P5201_00145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009164744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RsmD family RNA methyltransferase"
                     /protein_id="HRV96972.1"
                     /translation="MTRGKKGAPPKEVRPTSGKVLGALFSILGPLQGRSFLDLFSGTG
                     RVAYRAAQQGASFVAAVEILPVRARDIRSLFPAAPSFLLLAMDIRRALSFLERKGHRF
                     EVIFADPPYGAGWPGILGELLFPKSGGILVPNGVTVIEHSLREPIPSGENYRIIDRRE
                     YGETVLSFLASAVLPTDTSGERGEKV"
     gene            7318..7824
                     /gene="coaD"
                     /locus_tag="P5201_00150"
     CDS             7318..7824
                     /gene="coaD"
                     /locus_tag="P5201_00150"
                     /EC_number="2.7.7.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013048460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004595 - pantetheine-phosphate
                     adenylyltransferase activity [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pantetheine-phosphate adenylyltransferase"
                     /protein_id="HRV96973.1"
                     /translation="MKRCISAVYPGSFDPITNGHIYIAERAAALFDEVIVSVLLNPQK
                     KAAFTVDERKAMAREALSHLTNVKVGSFSGLLVDFLRQERSRIIIRGLRALSDFEYEF
                     QLALMNRQLAPDIETLFIVTDARYSYLSSHAVKEIYQFGGAVHEMVPPGVFRRLRERF
                     PLPHTLAP"
     gene            complement(7784..9016)
                     /locus_tag="P5201_00155"
     CDS             complement(7784..9016)
                     /locus_tag="P5201_00155"
                     /inference="COORDINATES: protein motif:HMM:NF017452.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase family protein"
                     /protein_id="HRV96974.1"
                     /translation="MVAEYNPLHNGHQHHIRRTREKVNPDGIVVVLSSYFTQRGEPAL
                     LSKWDRAESALHAGANLVLELPVFFSSHNAGVFASGAVDVLAATGLVQTISFGMEEPD
                     FDVAPLVDILVQEPITFKDSLKKHLNSGFSYVKARAEALASINAEYGVFVSSPNNSLA
                     LAYMEHIARKGLNIQCMPIQRTGAGYHNTSVTASGEESCFASASAIRRELLESGLDAV
                     AHTLPVPSFEILRRSMETGYYVLSTDLLWRMIRMVLLRTTKKNLSHSSEMTEGMENRL
                     LRYASECGSWDEFLRKCVTHRYPRARIQRQLIHLLLGIDHTENRTFQENGPAYIHPLA
                     ADKKGVEMLRAMRKKASLPIRGKLPLHAQGAEGKLASIECLAAEIWESLTPAFIPGTE
                     KKRHLIRGQEYAEEGNAP"
     gene            9116..10315
                     /locus_tag="P5201_00160"
     CDS             9116..10315
                     /locus_tag="P5201_00160"
                     /EC_number="2.7.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013048458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008776 - acetate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006085 - acetyl-CoA biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetate kinase"
                     /protein_id="HRV96975.1"
                     /translation="MKILVVNCGSSSLKYQLFDMTDESVLAKGLIERIGIEGARIKHT
                     KTGMDAVIKNREIPNHKVAFKVVVEALLDPEHGVLKSLDELAAVGHRVVHAGERYAGS
                     VLITHDVIDALEECVPLAPLHNPANLMGIYAVTEALPNIPQVGVFDTAFHQTMPGYAY
                     MYGVPYHYYEKYKVRRYGFHGTSHYYVSHRAAEILGKPVESLNIVTCHMGNGSSLAAV
                     KGGKSVDTSMGFSPLPGVIMGTRSGDVDPLLISYLSDQEGIDNKAMSHVLNKESGLLG
                     ISGVSSDLRDIEEAADSGNQRAKLAEDMLYYGVKKYVASYAAAMGGIDVLVFTAGIGE
                     NSARARESICAGLEFMGIKLDKEKNNVRGKERIVSTDDSRVKVIVIPTDEELVIARDT
                     KKIAIKN"
     gene            10343..10963
                     /locus_tag="P5201_00165"
     CDS             10343..10963
                     /locus_tag="P5201_00165"
                     /inference="COORDINATES: protein motif:HMM:NF014657.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF177 domain-containing protein"
                     /protein_id="HRV96976.1"
                     /translation="MPITEKPDSWRFEIDTPELGNEDAVTTALWEQEVLSRPGLGSAP
                     DSARAEILYDGLLFRFASPLKIEAEARWADDSLCVTIAVKAFFTTQCSRCLEPTDIEI
                     LDDFMYLYSLRKKTAANRNATEETDDEYQVVPVASWKNRLDISDQVWESLILTLPMQV
                     LCSRDCRGICPTCGKSLNSGECICSSETKDPRFEKLLAIQFDQDTE"
     gene            10982..11179
                     /gene="rpmF"
                     /locus_tag="P5201_00170"
     CDS             10982..11179
                     /gene="rpmF"
                     /locus_tag="P5201_00170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006583616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L32"
                     /protein_id="HRV96977.1"
                     /translation="MATPKSRVSHARTHKRKSEWIRSLSGPELTTCPHCGEVVMNYTA
                     CGECGFYRGRKVLETKTEAKE"
BASE COUNT         2721 a         2879 c         2954 g         2780 t
ORIGIN      
        1 ttcgatacgc ttcccaccga ccaaaaagga cggccgttca agatttatta ctgcacgcaa
       61 tccggcgtag aacctcctac atttatcttc ttcgttaacc atccggacct agcctctccg
      121 gcctttgaga accatatgga gaaggccttg agaggtctgg aggacttttc tggcacacca
      181 attcgcattt tttggcgtgg aaaagaggaa aaatgaagat tccgcttgac cggaattgca
      241 gttgctggta ataataaaga actgataggt tcggataccg caaggcttta tcaaaacgaa
      301 ccaggttcgg agtcagtttc agattcatat catatttgga ggaggctgta tacggtgacc
      361 aaggcagaac tcatcacaga agtagcgaag gcgaccggct tgaacaaaaa ggagtccggc
      421 gcggcggtag gagcggtttt tgaggcgatc gaagccgctc ttgaaaaggg cgagaagatt
      481 cagctcgttg gtttcggtac gtttgaggtc cgggaaagag cggctcgtga aggacgtaat
      541 ccccaggatc cgaagaaagt tatcaagatc cccgcgaaga agattcctgt tttccggccc
      601 ggcaaagctt tgaaggacaa ggtagagtag gcctctttcc ttcctttttt gcctcttccc
      661 gctcactccg gggagaggct ctttttttca aaggtcttta aaaaaaacca taacgccaac
      721 cactgttctc ccgtcagttc ctcggcccgt gcattttcat gtatctccgt acgttcaaaa
      781 gcctcgctcc agctaactga aggataaaaa ccgcgcagat tgttcgagag cttcttacgc
      841 ctttgagcaa aactggaacg gaggagattc ctccagaggc tatccttcgg gagagtcgca
      901 tgcattccgg aaagacggat ttcaagaagc gatgattcaa cagcaggaac cggacggaaa
      961 gactgaggat ggacggtccg aacacttcgc gcctttccca taagctcgag cgttatccca
     1021 agggggtagc gttcctttgt tcctggaggg gctgtaatcc tctctgccgc ctctttctgt
     1081 accataagga ggaaatagcc cgtcccggcc tccgggagtt gctccaggag cctccagagc
     1141 aggggagtgg taatgttgta tgggatgttt gctacaacct tcgttggaac gggagaaagg
     1201 gaagaatagt ccacggcaag cgcatctccc cacagaagag aaaacgtgtt tggatagagc
     1261 agagtgatgt cccgcagata ctccgaaagc ccttcatcga tttctacaga gaaaacgaac
     1321 gagcacggag aggccgcaag ttcccttgtc agcactcctt ggccggctcc tatttccaga
     1381 acggtatcgt ccacggaaag atcggcctgt tccagaatat gtcgaagtat attccggtcg
     1441 acaagaaagt tctgcccgag gcttttttta ttcttaaaac ccttttgttc tcttcccatt
     1501 ctgtcttttc gctcctcgac aaatacaaaa aaagcggggg tatgcccccg ctttaatatt
     1561 ctggtcggga cgagaggatt cgaacctccg accacctgca ccccatgcag gtacgctaac
     1621 caggctgcgc tacgtcccgt ttcgaccttg gtattctaca ttgaaacgcg cttgcggtca
     1681 atgatccaaa ttaaagtgga aatattccat gttgcgaaca tcagcccatg ctcaaaacag
     1741 ttcgccccaa gatgtacaaa aaaagaggag gaagtcgcct cccccctctc aacggtttta
     1801 cgcttcagcc tttacaggct gtttgcccat ctcggcaagc tgcttataga tttcgtaacg
     1861 ctcccgggca tcctttgccg ccttttcgaa gagctcgtcc gcggtatccg ggaactcctt
     1921 tttcaaggaa gcataacgaa cttcgctcat caggaagtcc ttaaagtctg ctttgggctc
     1981 cttggagtcg agtacaaacg ggttgctccc tgcctcggcg agttcggggt tatagcggta
     2041 gagatgccag tatcctgcct ccacggcttt tttcgtctga tcctgcgtct tgcccattcc
     2101 ggcgctgatg ccatgcgcaa tgcagggagc gtacgcaatg accagagagg gcccgttgta
     2161 ggcttccgct tctttaatgg ctttcatcag ctggttcttg tcggcgccca ttgcgacctg
     2221 agcaacgtaa acatatccgt aggtcatcgc gatccggccg aggtcctttt tctttacccg
     2281 tttgccggag gccgcgaatt ttgcgattgc cgccgtctgc gtggatttcg aggactggcc
     2341 gccggtgttg gagtacactt cggtgtcgag tacgaagaca ttgatgtctt cacccgacgc
     2401 aagcacgtgg tcgaggccgc cgaaaccgat atcgtatgcc cagccgtcgc cgccgaaaat
     2461 ccagacggac tttttaacaa gataatcctt gtggcaggcg atttcgcaga gcagcttgtt
     2521 cccttcttcg ccgatgtcgc gatcaagaat agcaagcacc ttttctgcgg cagctttgga
     2581 tttgtctccg tcatccttcg actcaagcca ctctgaaacc acggctttta catcctcggg
     2641 gatgtcgagc gccagaagac cgttcattgc atcgatgaca tattgcattg tggcgttgac
     2701 agagagcttc atgccatagc cgtattccgc gttgtcctca aagagggagt tggcccatgc
     2761 aggtccctgc cccttggagt tcttcgtata gggcatgttg ggagccgatc cgccccagat
     2821 cgaagagcag cccgtggcgt tcgcaacaat catccggtcg ccgaagagct gtgtaacgag
     2881 cttaatgtac ggcgtctcgc cgcacccggc gcacgctcct gagaactcga ggagcggctg
     2941 ggaaaactgg cttcccttga ccgtaaactt gttcgtctcc gcagacttgt cggaaacctc
     3001 ttctacggca tagagccagt tcggtatctc tttttcctga gaaacgatcg gtttcatttc
     3061 gagcgcgccc actgggcaga tatcggcgca gttgccgcag cccatgcagt cgagcgtgtc
     3121 aacctgcatc ttgtacttgc agccggggaa ctcttttcct ttgacttcga ctgcgccgaa
     3181 gccttgaggc gcggccgcga gttcctgctc gttcagaata aacgggcgaa tggcagcgtg
     3241 cggacagacc atcgagcact ggttgcactg gatgcacttg tcgcttttcc attccggaac
     3301 atttaccgca acgccacgtt tctcgaaagc ggcggttccc gaagggaatt tgccgtcttc
     3361 gcgtccgagg aatgcgctga caggaagaga atcgccctgc tgtgcattga tgggcatgca
     3421 gacatccttg acaaaatcgg gaatatccga cggcagaccg gaaagaagca ccggttcgga
     3481 cgtcgcgttc gcccactcag caggcacgtc gatcttcttg atcgcattga tgcctgcatc
     3541 cactgccttg tagttcatct cgagaatggc ctcgcccttc ctgctgtagg tgtggtcgat
     3601 ggcgtccttc atatatttca cggcgtcgtt tataggaatg acattggtca gcttgaagaa
     3661 tgcggcctgc atgatcatgt tgatcctgtt gccaagaccg agctcctcgc cgatgtccgt
     3721 tccattgagg atataaaact gaatatcgtt gtttgcaagg gcgcgtctca tccttgaagg
     3781 aagatgcttc tcaagctttt cgatagtgtc ccactgggtg ttcagaagga acgaaccgcc
     3841 ttttttcagg ccgccgagaa catcgtagag attgacatac tcctgcttgt ggcaagcgat
     3901 gaagtcggca gtatcaatga gataggtgga cttgatcggt tttttgccaa aacgaaggtg
     3961 ggaaacggta atcccgccgg attttttgga atcgtacgca aaataccctt gtgcgtaaag
     4021 gtctgtttcg tcgccgatga ttttaatcga gttcttgttg gcgcccacag ttccatcgga
     4081 cccaaggccc cagaatttgc atctgattgt tccttcaggg gctgcgacaa tttgctcgtt
     4141 taccgggagt gacgtgccgg taacgtcgtc gacgatgctg atggtgaaac gatccctggg
     4201 ctgataaagc ttcagattgt cgaaaacgga tttgatatgt gtcggtgtgg tgtccttgga
     4261 accgaggccg tagcgtccgc cgacaacgat cggtttttcc gctttgtcct tgaaaatagc
     4321 gcaaatatcc tcaaagaggg gctcgccgag agcgccgggc tccttgcatc tgtcgagcac
     4381 agcaatgcgc cttacggatg cgggaagcac gtcgaagaag tatttctcgg agaaggggcg
     4441 atacagatgc acttctacaa cgcctgtctt ctcgccgcgc gcgttgaggt agtcgaccgt
     4501 ttcctcaatc gcctgacaga cagaacccat tgcgatgatg atgttttcag cgttcgggtc
     4561 gccgtagtag ttgaacgggt gatactctct gcctgtgagc ttcttgatct cctgcatgta
     4621 cgactcgacg atgtccggga taacagtata gaatctgttg accgcttctt tcgcctggaa
     4681 gaagatgtcg gggttctgcg cggttccctt ctggtagggg tgttccggat tcattgcgcg
     4741 cgccttgaat tttgcaagcg cgtcatagtc gacaagtttg gcaagatcgt cgtactcaag
     4801 cacgtcgatc ttctgaattt cgtgagaggt tctgaagccg tcgaagaagt tgacaaaagg
     4861 aagactcccc ttgattgcgg agaggtggga gacagcggtc agatccatta cttcctggac
     4921 gctgccggat gcgagaaatg caaaacccgt tccgcggcaa gccatgacgt cgctgtggtc
     4981 gccgaagatg gaaagggcat gcgcggcgac tgagcgggca gtcacgtcaa agacaccggg
     5041 aagtagttcg ccggcgattt tgtacatatt cggcatcata agcaacaaac cctgagatgc
     5101 tgtgaaggtg gtcgaaagcg atccggctga aagagaaccg tgcacggcgc ctgcggctcc
     5161 cgcttcagac tgaagttccg tgaccctgac agtcttgccg aaaatattct tcctgccgtg
     5221 ggcagcccat tcatcgacaa cttcgggcat attggaggac ggcgtaatcg ggtaaattgc
     5281 agcaacctcg gtgaacgcat atgaaacatg cgctgcagca gtgttgccgt ccatcgtttt
     5341 ccaatttctc ttcattgatg taccccctct tgaattacag gaggggcagc gttgtcgcaa
     5401 cccgctcctg ttttattatt cttgatccat agacgcaata agccccattg tgatctttcc
     5461 agcgggctct tgtaatgtcc gcacaaatac gccgaaggtc agatgggaat tcgacttccg
     5521 gttaacaccc ttctatttta caacaagatc gggattttgc attctgtata atccgcactt
     5581 ctggaaaaaa acgacaacca aaaagtattt ttttcacgta ttgatattac cataaacctt
     5641 ctacgggtca attcgttcat ttcactccaa acgcttcact tttgccgatt tcgttccgaa
     5701 ggctgctata atcgtatgca ttccggtacg agtttgctca aggagaggtt ggaattgtga
     5761 tcgacatacg agaaattatt atcatgcctg aaaagcaaca gcttcctctg aatatactga
     5821 gagcgagagg ggaaacgcgc gtatttcttg aaataggttt cggaaacggt gaatttctcg
     5881 ttcatcttgc acggcaaaac ccggatgccg ttttttgggg cgtcgagatg tcccggtcgt
     5941 gcgttctgcg cgctctgaaa agaattcgca gagaagaact gcgaaacatc ttcctcctgt
     6001 gcggcgatgc gcgtttcatt ctgaaagaat gccttccaaa agaatctctc gacggcattt
     6061 acatgaattt tccctgtcct tggcctaaga aaaaacacac taaaagaaga gtttccagcg
     6121 gagattttcc ctcggagatt gcgagcgttc tcaagaaggg ggcgttcttt gaacttgtaa
     6181 ccgacgagga atggtacggt atggaagtgc gaaatgtgct ggccgctcat cctttgcttg
     6241 aacttacgga gtggaagata aatccgccca ggccggttac aacaaaatac gaacgcaaat
     6301 ggatggagat ggggaaacgt atttttcttt ccagactgca gaaaaaagaa gcggaatcat
     6361 ctgtcggata ccgctgtcta gggaggacgg aggacgtgca tattcgagtt ccgggaaagg
     6421 gatctttaaa aaaattcgtg aacgatatac ataacatggg aggagaaaag ggcgacgctc
     6481 tctgggtgta taaaaggtat tacgaatctg cggaaggaaa cgcttatatt atcgaaacag
     6541 tggccggcga cggctccttc gaacaaaaat ttttcatcca gatcgtagag cgcgaagagg
     6601 acgccctcat aaaaatagcg ccgtattcga gcccgttttt gactgattca gtacgcgggg
     6661 ctctcgaagg tatggctcac ttgattgaga cgcactatct atcgatcgcc tcctcctgcg
     6721 ccgcttcact tgagcaacct gctggaaagg cgttttcgca gtgaccagag gaaagaaggg
     6781 ggcgccgccc aaggaggtcc gccctacttc cggaaaggtt ctaggggcgc tctttagtat
     6841 cctcggtccc ttgcaaggtc gttctttcct cgaccttttc tccggaaccg gcagggttgc
     6901 atatcgagca gcccaacagg gagcttcttt tgttgcagcg gtggagatat tgcccgttcg
     6961 tgcccgggat attcgttctc tattccccgc cgccccctct tttcttctcc tcgcgatgga
     7021 tattcgaaga gcgctttctt tcctggaacg gaaaggtcat cgttttgaag taatctttgc
     7081 cgacccgccg tatggtgcgg ggtggcccgg catcctcggg gaactgcttt ttccgaaatc
     7141 aggaggtatt ctcgttccga acggggttac agttatagag cattcgctcc gggagcccat
     7201 cccttccggc gaaaactaca ggatcatcga tcgccgcgag tacggagaga cggtcctttc
     7261 ttttcttgca tcggccgttt tgcccacaga tacttccgga gagagaggag aaaaagtatg
     7321 aagagatgta taagcgcagt ctaccccggg tcgttcgatc ctatcaccaa cggacacatc
     7381 tacatcgcgg agagagcagc ggcgctcttc gatgaagtca ttgtaagcgt tctcttgaat
     7441 cctcagaaaa aggcggcgtt taccgtcgac gaaaggaaag ccatggccag agaggcgttg
     7501 agccatctga ccaacgtcaa agtcggttcc ttctccggtc ttctcgttga ttttctgcga
     7561 caggaacgga gccgtatcat catcaggggg ctccgggcgc tctcggattt cgagtacgag
     7621 tttcagctgg ctctgatgaa cagacaatta gcccccgata tagaaacgtt gttcatcgta
     7681 accgacgcaa ggtactcata tctttcaagc catgctgtaa aggagatata tcagttcgga
     7741 ggagccgtcc acgaaatggt tccgccgggg gtcttccggc gtctcaggga gcgtttccct
     7801 cttccgcata ctcttgcccc ctgatgagat gccttttttt ttcagtcccg ggaatgaagg
     7861 caggggtgag actttcccag atttctgcgg ccaaacattc aatagaagcc aactttcctt
     7921 cggctccctg cgcgtgaagg ggcagcttcc ctcgtatggg aagcgatgct ttcttccgca
     7981 ttgcccgaag catctccact ccctttttat ccgctgcaag cgggtgaata tacgcaggac
     8041 cgttttcctg aaaagtccgg ttttcggtgt gatcaatacc cagcagaaga tgaatgagct
     8101 gccgttggat tctggctcgc gggtatcgat gagtcacgca tttcctcaaa aattcatccc
     8161 atgagccgca ttctgaggca taacgaagca acctgttttc catcccttcc gtcatctccg
     8221 atgaatggct caaatttttc ttcgttgtgc gcagcagaac catacgtatc attctccaga
     8281 gcaaatcggt cgagagaacg taatacccgg tttccataga ccgccgcaga atttcaaagc
     8341 ttgggaccgg aagcgtgtgc gccacagcat cgagtccact ttcaagtagt tcgcgacgta
     8401 tagcggaggc gctggcaaaa caggattctt cgccggatgc ggtaacggaa gtattgtgat
     8461 aaccggcgcc ggtccgctga attggcatgc actgaatgtt cagccctttt ctcgcaatat
     8521 gctccatgta cgccaatgcg agtgaattat taggggaaga gacgaaaacg ccatattcag
     8581 cattgattga agcaagagct tccgctcgcg cttttacata ggaaaatccc gaattcagat
     8641 gttttttcag agaatccttg aaagtaatcg gttcctggac tagaatatca accagtggag
     8701 caacatcaaa atcaggttct tccatcccga aagaaatggt ttgaaccagg ccggtggctg
     8761 caagaacatc gaccgctccc gatgcaaaga cgcctgcatt atgactggaa aagaaaacag
     8821 gcagttccag aacaaggtta gctccggcat gaagcgcgct ttccgcacga tcccacttac
     8881 ttagaagcgc gggctctcct ctttgtgtga aataggatga gagcacaaca acaatgccgt
     8941 cgggatttac tttttcccgc gttcggcgta tgtgatgctg atggccgtta tgcaatggat
     9001 tatactcggc tacaatacca acataatttc catatatggc gaccggcctc cttttgttta
     9061 ttacaataca acctgcgaaa gcgtccggtc aagatgagag gaaggggtag tattcatgaa
     9121 gattcttgtt gtaaactgcg gtagttcatc tctcaagtat cagcttttcg atatgaccga
     9181 cgaatctgtg ctggcaaagg ggcttattga gagaataggc attgaaggag cccgaatcaa
     9241 gcacacgaag accggaatgg acgccgtaat caaaaacaga gaaatcccca atcacaaagt
     9301 tgccttcaaa gtcgtcgtcg aggccctcct tgacccggaa catggcgtat tgaagagcct
     9361 cgatgaactc gccgccgtcg ggcacagggt tgttcacgcc ggagagcggt acgcaggctc
     9421 ggtccttatc acccatgacg tcatcgatgc tctggaagag tgcgtccctc ttgctcctct
     9481 tcataacccc gccaacctca tgggcattta cgctgttaca gaagcccttc caaatatccc
     9541 ccaggtaggc gtattcgaca cggcgttcca ccagaccatg cccggatatg cctatatgta
     9601 cggcgtcccc tatcattact acgagaagta caaagtgcga cgctacggct ttcacggtac
     9661 cagccactac tatgtttccc acagagccgc tgaaattctt ggcaaacctg tcgagtcgct
     9721 caatattgtc acatgccaca tggggaacgg cagctctctt gccgcggtaa aaggcgggaa
     9781 gtcggtcgat acaagcatgg gattcagccc ccttccaggc gtcattatgg ggactcgatc
     9841 cggcgacgtt gacccgctgc tcatctctta cctttcagac caggaaggaa tcgacaacaa
     9901 ggcgatgagt cacgtcctga acaaggaaag cgggcttctt ggtatttccg gcgtcagcag
     9961 cgatctccgt gatatagaag aagcggccga ttccggaaac cagcgggcca aacttgccga
    10021 ggacatgctc tattacggcg tcaagaaata cgttgcgtcg tatgctgccg ccatgggagg
    10081 cattgatgtg cttgtcttca ccgccggcat aggggaaaac agcgcccgcg cccgcgagag
    10141 catctgcgcc ggccttgaat ttatgggaat caaattggac aaagagaaaa ataacgtccg
    10201 tggtaaagaa cgcattgtca gcaccgacga cagccgtgta aaagtaatcg taataccgac
    10261 ggacgaagag ctcgttatag ctcgggatac taagaagatc gcgattaaaa actagtcgct
    10321 agagccctag gaggggaagg acgtgccaat aacggaaaaa ccggactcat ggcgttttga
    10381 aattgatacc ccggagcttg ggaatgagga tgcggttacc acggcccttt gggagcagga
    10441 agttctgtcc cggcccggac ttggttccgc acccgattca gcccgggcgg aaattcttta
    10501 cgacgggctt cttttccgtt ttgcttcccc gctgaagata gaagcggaag cgcgttgggc
    10561 agacgatagt ttgtgcgtta caattgccgt aaaagctttt tttacaacgc aatgttctcg
    10621 atgtcttgag ccgacagata ttgaaattct ggatgatttc atgtatcttt attctttgcg
    10681 gaagaagact gccgcaaacc gtaacgctac ggaggaaacg gatgatgagt atcaagtagt
    10741 accagtcgct tcctggaaaa atcgtctgga catttcggac caggtctggg aaagcttgat
    10801 tcttactctt ccgatgcagg tgctctgctc tagggattgc cgcggcatat gtccgacgtg
    10861 cggaaagtct ctgaactcgg gagaatgtat ttgctcttcc gaaacaaagg acccgcggtt
    10921 tgaaaagctt cttgcaatac aattcgatca ggatacagag tgaaaaggag gggaatgcag
    10981 aatggcgacg ccgaaaagca gggtatccca cgcccgtaca cataaaagaa agtcggagtg
    11041 gatccggtcc ttgtccggcc ctgagctgac cacatgtccg cactgcggag aagtggtaat
    11101 gaactacacg gcttgcggag aatgcggatt ttatagaggc cgaaaagtac tcgaaaccaa
    11161 gacggaagca aaagagtagc ttgcagacta tactcagaac ggtttagttg caaaagagag
    11221 aggggaaacc ctctctcttt ttgtttcatc ttccatgctt cacctattga ccgctcgctg
    11281 taacccaatt atacttacct tgtcttgaga gcagatgtta tcatcaggtg gtta
//
LOCUS       DAOTIL010000005        11208 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminobacteriaceae bacterium isolate AMR_MDS_3026
            NODE_6913_length_11208_cov_3.545593, whole genome shotgun sequence.
ACCESSION   DAOTIL010000005 DAOTIL010000000
VERSION     DAOTIL010000005.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868050
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminobacteriaceae bacterium (wastewater metagenome)
  ORGANISM  Aminobacteriaceae bacterium
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae.
REFERENCE   1  (bases 1 to 11208)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11208)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:19:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,224
            CDSs (total)                      :: 3,198
            Genes (coding)                    :: 3,184
            CDSs (with protein)               :: 3,184
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11208
                     /organism="Aminobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6913_length_11208_cov_3.545593"
                     /isolate="AMR_MDS_3026"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:3034609"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            221..1870
                     /locus_tag="P5201_00175"
     CDS             221..1870
                     /locus_tag="P5201_00175"
                     /inference="COORDINATES: protein motif:HMM:NF014904.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="[Fe-Fe] hydrogenase large subunit C-terminal
                     domain-containing protein"
                     /protein_id="HRV96978.1"
                     /translation="MPTKRAVKAIRVERGQAAVIPELCVACGRCVEVCPFQAVKPRLD
                     LPFVKRMIASGRRVYASMAPTWACEFPGLDSGSLVAALKKLGFTGVGETALGAHEVCE
                     SLAKTLSEAEPGLFLSTTCPSVVSYVERYLPELLGSLSTVPSPVEAHCRIFKDRVDPE
                     GAVVFIGPCIARKDESDRSVKTIDAALTFRELRRWLHDESVAPEKCVPGPEDHFFPEP
                     TGDGFLYPVEGGMCDMIRANGVDKKVRFVTVSGLQNLRRYLRGLSPEGLGAPVFLECF
                     ACLGGCINGPGGTWDEPGLLRRNRVEAYANLSNAPRHTIFLEDEGSAMREEDMKGALR
                     RIGKTRPEDEVNCGGCGYASCRELAAALALGRAEPEMCVIHMRNQAQKKANALLRTMP
                     SGVVIVDKSLHIVECNERFARLFGESAMLVYRAEPGLKGCILGRILPFTHLFELVLEK
                     GQDIHLDHYRTGDTLLDITLFPIEPGETVGAVMLDVTHKELKRDKIAQRAAEVIERNI
                     LTVQEIACRLGEHMADTEMLLRSIAEGYSESDEDVSQEDAR"
     gene            1867..3048
                     /locus_tag="P5201_00180"
     CDS             1867..3048
                     /locus_tag="P5201_00180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005659985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SpoIIE family protein phosphatase"
                     /protein_id="HRV96979.1"
                     /translation="MKQEEPLFLDVDFDQRKKAGQYVCGDSFFSRKLPGRNRVVSVLS
                     DGLGSGVKASILSSMTGTMALRFMEGDMEILRSAEVMMDALPVCRKRGISYATFTIVD
                     AVLHGWTRVIEMDNPSFMLVRNGRLLAPGKREMASSRWKDRKITVNEFFARPEDRIIL
                     FSDGVTQAGMGSWANPTGWGQEKCAAFILDTLRERPGISSRELSRRVVSEALVREPHE
                     RAGDDVTCAVMYFRSPRKLLVLSGPPFEKKRDAEFARMLDTFDGRRAVCGGTTAEIVA
                     RELGRPLVTDLATARGGLPPISTMEGADLITEGILTLTEVSRILEGKKITREKTSASR
                     LAALLEESDRIRFVVGTRVNEAHQDPTLPVELEMRRNIVRTIARTLEERYLKEVELTF
                     V"
     gene            3082..5853
                     /locus_tag="P5201_00185"
     CDS             3082..5853
                     /locus_tag="P5201_00185"
                     /inference="COORDINATES: protein motif:HMM:NF016292.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate binding protein"
                     /protein_id="HRV96980.1"
                     /translation="MHVRNAKNGTITTLYERSLKRFEYFTRRLVNEMHYRRTPVWILR
                     PACVLFAAFILLASSSFAAAGAEKRILLLHSFHQGYEWTDSLHQGVVEELQNSGDKDI
                     ELFVEYLDCIRNPGPAHEEMMAELFRRRYAERGVLFDAIICTDDDALVFLRERGESLF
                     GNVPKVFCGINDFTPEHIKGMTNVTGVNESISVGETIEAALNLHRSTKTIAVVSGSRR
                     TEKRNLELVRQSAPSFSDAVQFIWLDELEPHALQKRLGELNPESSAVLMLAYLLTPSG
                     QALTVRENMELITEATSVPVFGCWDFLLPHGAVGGKVVHGHSQGAAAASLALRILAGE
                     RADSIPVVLESPNRYVFNAAMLQKFAIPEALLPPDSQIVYRTAASLLSNWETLSRKFF
                     FGYDLFAKNTTPMMLIDPDTGIILDGNHSARGFYGYPELKGMNIAQINMLSPEEVSRE
                     MERARSRNNTSFDFKHRLADGSVRDVGVHSSPVFIDGREILLSLVIDETERVTAQEAL
                     GQRNRWIVALVVSALLLQSVVLLQLLRSAAQRKKAEKALRESERKLGALFASMTEVVA
                     LHDLVFDEEGRPANYRITGCNAAFTKSIGIPEEAAVGRLATEVYGTEKAPYLQEYGKV
                     AMTGEPSTFETYFAPLDKHFFISVVSPGENSFATVTTDITSIKKAEQLIEAKNKELEQ
                     VVYVASHDLRSPLVNVDGYSRELEYSVEDLLRALDDESLAPPERERAIRALLPDMTDS
                     LRRIRGSAKQMDALLRGLLKLSRLGRAALSVGSVDMNELLAELSSSLQFQVREAGAAL
                     RVGRLPSCRGDAVQLTQVFANLLGNALKYLDPQRPGEIVVEGRIEQDRSVYLVRDNGI
                     GIAPQHQEKIFQLFHRLNPSANEGEGLGLTIVRQILGRLDGEVVVESTPGTGSTFTIT
                     LPAASKKGE"
     gene            5874..6320
                     /locus_tag="P5201_00190"
     CDS             5874..6320
                     /locus_tag="P5201_00190"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HRV96981.1"
                     /translation="MHNEVIILIAEDDEGHAGLIRKNLARAGISNHVEHFKDGQEIID
                     FLFRIGNGPHRRSHTPYILLLDIRMPKLDGTEVLKRIKEDHELRKMPVIMITTTDDPR
                     EVERCHELGCSSYITKPVEYDAFVNAVRQLGLYLAVVQVPQVNGVH"
     gene            6341..>7550
                     /locus_tag="P5201_00195"
     CDS             6341..>7550
                     /locus_tag="P5201_00195"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HRV96982.1"
                     /translation="MDGRAEHGDAPARNVLVVEDNEDLRSLIVRRLHRAGYEAFGVSL
                     GCEAVEKAFERPGTALLIDHNLPDMSGQELVALLKERGAAPPFIIMTGQGDERLAVQM
                     MKLGAFDYLVKNSELLDKLPETLGRLFGELETERRILEDRSILLDNIRTQVWYLTDDH
                     TYGAVNKAHADFCGLRQEMMSFRDMFELFPREVAELHRSNAMKVFGTAEPLTVELWLP
                     SSTGELRLLSIYKSPKLREDGSVEYVVCSAEDVTERKRAGDALRESEERFRKLFSSVQ
                     SVSVQGYAADGTVRYWNSASEQLYGYTADEAIGGNLLDLIIPRGMREAVRNDIHDMVE
                     KGEGRAAERLALKKKDGSRVDVLSSHVVVDYIDGRRELFCLDIDIGEQIRMEEALRAA
                     KENAEWLGQQA"
     assembly_gap    7551..7650
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <7651..8895
                     /locus_tag="P5201_00200"
     CDS             <7651..8895
                     /locus_tag="P5201_00200"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HRV96983.1"
                     /translation="EEALRAAKENAEWLGQQAEAASKAKSAFLANTSHELRTPLNGAI
                     GFLELLADTPLNELQKEYVGYIRTSAYTLLEVISEVLDISKIESDRFDLEYAQSDVFE
                     VMRKAVDAVRGTVLRKELRLELRIEEGTPRYAVVDPLRLKQVLVNLLGNAVKFTEKGF
                     VELALQCVALDAGRCAYTFSVRDTGIGIAPEVHRKLFEPFFQADASNTRKYGGVGLGI
                     TICEALLQKMDSRLQLESAPGQGSRFFFTLCARCDAEPEETTNGERTNLLPAKGVPLP
                     LRTNRQAVVLLAEDQRLNRRLLRILVSKLVPGARIIEASDGEEAVRFFRSEAPDIVFM
                     DLQMPLKDGCEAVAEIRSLERASSGGTAKAGIPIIAVTADVQKETRTFCLENGMDDFI
                     TKPVDAWSLRCVLERCLESVLL"
     gene            9227..>11208
                     /locus_tag="P5201_00205"
     CDS             9227..>11208
                     /locus_tag="P5201_00205"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HRV96984.1"
                     /translation="MPLRRLFLLLMMLLLAFVLVLTSFTSQRTTLKAFDDVERRFFIQ
                     DMARVQNRLDEELRVLRNYAADWGAWDSMYAFVEKGCKHRFDEMFDGQAMLAAGMEAL
                     LIFDTGRRLLFSIFEGECAAERKLPDHVSKGLKDAVPSLMERSSDGAFAEILLLGDAA
                     YLFGVSPVLRTNRTGPSQGVLLIGRSLGKLLPEMRKSLGTKFDLLPGHAADVPDRSAG
                     DVRIVSGEGEGAAVWQGRGNEFSVRIDVPRLIRQRGLEAVESSRLWILLTGAGTLGLV
                     LLLLDRVILRRLRKLREVSERIVAGESLTLRVDEEGPEEIFALSRSFNMLLQTLHDLV
                     MKIPDPFILADADGAITLANDAAHTVLSYGTDLKGTPLSSVLVKKEDDPRAGNNRTDA
                     EKDTEAKDGHSVFEASLLRADGSLLPVEVHRRSFTFGALPLSLLLARDLKERKQFEER
                     LARKAYFDDLTGLPNRHSLLKAINRLLNDGTRTRMFSLAVINMDHFKLVNAQVGNLNG
                     DRILLLIAEKIKELQEPEAQLFRTSGDEFALLFPLPEGGESEEMLLMDRIRKGIATPC
                     EVGDETVFPSASIGVLVDIAEGKNASGAIDAALHAVTQARKNGIGRIARYVAPEVREY
                     TRGPNLLTLQAELQVALERHEFVPYFQPVYDIVSEK"
BASE COUNT         2218 a         3314 c         3538 g         2038 t
ORIGIN      
        1 cgttgaggat ctccagcacg gcgcgcagga agcgcgggcg cagccacgcc gggatcggga
       61 tctggacgcg caggaacagc agggcgatgg cggcggccga aaggaccagg gccagcagga
      121 agacgtggag gttggcgccg gtaaggtgtt gcatcgcccc attgtgcgtg ccgggcgttg
      181 cttgtccacg ccaaccgttc agaggctggg gttgacccgg ttgccgacga aacgggcggt
      241 gaaggcgatc agggtggagc gggggcaggc ggccgtgatt cccgaactct gcgtcgcctg
      301 cggccgatgc gtggaggtct gccccttcca ggcagtgaag ccgaggctcg atctcccctt
      361 cgtgaagcgg atgatcgcct cggggcgcag ggtgtacgcc tccatggcgc cgacgtgggc
      421 ctgcgagttc cccggtctcg actccggctc gctcgtggcg gcgctgaaga aactcggttt
      481 caccggagtg ggggagaccg cgctcggcgc gcacgaggtc tgcgaatccc ttgcgaagac
      541 gctctcggag gcggagccgg ggctctttct ctccaccacc tgtcccagcg tggtgtcgta
      601 cgtggagcgc tacctccccg agctgctggg aagcctctcc acggttccct ccccggtcga
      661 ggcccactgc cgcatcttca aggatcgcgt ggatcccgaa ggcgccgtcg tcttcatcgg
      721 cccctgcatc gcccgcaagg acgagtccga caggtcggtg aagaccatcg acgccgcgct
      781 caccttccgg gaactgcgcc gctggctgca cgacgaatcg gtcgcgccgg agaagtgcgt
      841 tccggggccg gaggatcact tcttccccga gcccaccggc gacggattcc tctaccctgt
      901 cgaggggggc atgtgcgaca tgatccgtgc gaacggagta gacaagaagg tgcgcttcgt
      961 cacggtctcg gggctgcaga acctgcgccg ctatcttcgg gggctctctc ccgaagggct
     1021 gggcgcgccc gtcttcctgg agtgcttcgc ctgtcttgga gggtgcatca acggacctgg
     1081 cggcacgtgg gacgagccgg ggcttctccg gcgcaaccgc gtcgaagcct acgcaaacct
     1141 ctcgaacgct cccagacaca ctatctttct tgaggatgag gggtccgcga tgcgggagga
     1201 agacatgaaa ggagcccttc gccgcatcgg gaagacgcgc cctgaggacg aggtgaactg
     1261 cggggggtgc ggctacgcct cctgccggga gctcgcggcc gcgctggcgc ttggtcgggc
     1321 ggagcctgag atgtgcgtca tccacatgcg gaaccaggcg cagaagaagg cgaacgcgct
     1381 tctccggacc atgccctccg gcgtcgtgat cgtggacaaa agcctgcaca tcgtcgagtg
     1441 caacgagcgc ttcgcccgtc tcttcggcga gagcgcgatg ctcgtctacc gcgccgagcc
     1501 ggggctgaaa gggtgcatcc tcggcagaat cctgcccttc acccacctct tcgaactcgt
     1561 gctcgaaaag gggcaggata tccacctcga ccactaccgc accggcgaca cgctgctgga
     1621 catcacgctc ttccccatag aaccggggga aacagtcggc gccgtgatgc tcgacgtcac
     1681 ccacaaagag ctgaagcgcg acaagatcgc ccagcgcgcc gcggaggtca tcgaacggaa
     1741 cattctcacg gttcaggaga tcgcgtgcag actgggggag cacatggcgg acacggagat
     1801 gctgcttcgt tcaatcgccg aggggtattc cgaatcggac gaggacgttt cgcaggagga
     1861 cgcgcgatga aacaggaaga gcctcttttt ctcgacgtcg atttcgacca gcgaaagaag
     1921 gccgggcagt acgtctgcgg cgacagcttc ttctcccgca agctgccggg gcggaacagg
     1981 gtggtttcgg tgctctccga cggtctcggc agcggagtga aggccagcat tctctcgtcg
     2041 atgacgggga cgatggcgct ccgtttcatg gagggggaca tggagatcct ccgctcggcg
     2101 gaggtgatga tggacgcgct ccccgtctgc cggaagcgcg gcatcagcta cgccaccttc
     2161 acgatcgtcg acgcggtgct ccacggctgg acgagggtga tcgagatgga caacccctcc
     2221 ttcatgctgg tgcggaacgg acggctcctc gcccccggaa agcgggagat ggcctcctcc
     2281 cgctggaagg accggaagat tacggtgaac gagttcttcg cccgcccgga agaccggatc
     2341 attctcttct ccgacggggt gacgcaggcc ggaatgggat cgtgggccaa cccgacgggg
     2401 tgggggcagg agaagtgcgc cgccttcatc ctcgacacgc tccgggagcg cccgggcatc
     2461 tcctcgcggg agctgtcgcg ccgggtcgtc tccgaagcgc tcgtcaggga gccgcacgag
     2521 cgggccgggg acgacgtcac ctgcgccgtg atgtacttcc ggtcgccgag gaagctgctc
     2581 gtgctctcag ggccgccgtt cgagaagaag cgggacgccg agttcgcccg gatgctggat
     2641 actttcgacg gacgtcgagc cgtctgcggc ggtacgaccg cggagatcgt cgcccgcgaa
     2701 ctgggccgtc cgctcgtcac cgaccttgca accgcgcgcg gcggcctgcc cccgatctcg
     2761 accatggagg gggccgacct gatcaccgag ggcattctca cgctgacgga ggtctccagg
     2821 attctcgaag gaaagaagat tacccgcgag aagacctcgg catcgcgcct cgccgcgctt
     2881 ctggaggaga gcgaccgtat ccgcttcgtc gtggggacga gagtgaacga ggcgcaccag
     2941 gaccccacgc ttccggtgga gctggagatg cgccggaaca tcgtccgcac catcgcccgc
     3001 acgctggagg agcgctacct gaaggaagtg gagctgacgt tcgtgtagaa aacggtgttt
     3061 caccggcgga gggccccgga agtgcatgtg cggaacgcga aaaatggtac aataacaaca
     3121 ctttacgagc ggtccctgaa gcgtttcgaa tacttcaccc gaaggctggt gaacgagatg
     3181 cactacagac gtactcctgt ttggatcctt cgccccgcgt gcgtgctctt tgccgcgttc
     3241 atccttcttg catcgtcctc cttcgcggcc gccggggccg agaagcgcat tctcctcctg
     3301 cactcattcc accaggggta cgaatggacc gactcgctcc accagggggt cgtcgaggag
     3361 cttcaaaact ccggcgacaa ggatatcgaa cttttcgtgg agtacctcga ctgcattcgc
     3421 aaccccggac cggcccacga ggagatgatg gcggagctgt tccggcgcag gtatgcggaa
     3481 cgcggcgtcc tcttcgacgc gatcatctgt acggacgacg acgctctggt ctttctgcgg
     3541 gagaggggag aatcgctctt cgggaacgta cccaaagtct tttgcggcat caacgatttt
     3601 actccggagc acatcaaggg gatgaccaac gtcacggggg tcaacgagtc catttcggtc
     3661 ggggaaacga tcgaggcagc tttgaacctg caccgttcga cgaagacgat agccgtcgtc
     3721 tcgggctctc ggcgcaccga gaagcgcaat ctggagctgg tgcggcagag cgccccctcc
     3781 ttcagcgacg cagtgcaatt catctggctg gatgagctgg aaccgcacgc gctccaaaaa
     3841 cgtctcggcg aattgaaccc ggaatcatcc gccgtgctca tgctggcata tctgctcacc
     3901 ccgtcggggc aggcgcttac cgttcgcgag aacatggagc tcattacgga ggcaacctcc
     3961 gtcccggtct tcggatgctg ggattttctg ctccctcacg gggcggtcgg gggcaaggtc
     4021 gtgcacggac actctcaggg tgcggccgcc gcgtcgctgg cgcttcgcat tctcgccggg
     4081 gagcgtgcgg actccatacc ggtagtgctc gaaagcccga accgctacgt cttcaacgcc
     4141 gccatgcttc agaaattcgc aatccccgag gcgttgcttc ccccggattc gcagattgtc
     4201 tatcgcactg ccgcaagcct cctttcgaac tgggagacgc tttcccgaaa gtttttcttc
     4261 ggctacgacc tctttgcgaa gaacaccact cccatgatgc tcatcgaccc ggatacgggg
     4321 atcatactcg acggaaacca ttccgcccgc ggcttttacg gctatccgga gctgaagggg
     4381 atgaatatag cgcagatcaa catgctctcc cctgaggagg tttccaggga gatggagagg
     4441 gcgcgcagcc ggaacaacac ttcctttgat ttcaagcatc gcctggccga cggaagcgtt
     4501 cgcgacgtcg gcgtgcactc ctcgccggtg ttcatcgatg gccgggaaat cttactctcc
     4561 ctggttattg acgagaccga gagggtgact gcgcaagaag cgctggggca gcgaaaccgc
     4621 tggatagtcg cgcttgtcgt ttccgccctg ctgcttcagt ccgtcgtgct gctgcagctg
     4681 ctgcgaagcg ccgcgcaaag aaagaaggcg gaaaaggcgc ttcgcgagag cgaacgaaaa
     4741 ctcggcgcgc tcttcgcctc gatgacggag gtcgtcgcgc tccacgacct ggtcttcgac
     4801 gaggaaggcc gtcctgcgaa ctaccggatt accgggtgca acgccgcgtt cacaaagtca
     4861 atcggaatcc cggaggaagc ggccgtcgga cgcctggcga ccgaggtcta cggtacggag
     4921 aaggctccgt atttacagga gtacggcaag gtcgccatga cgggagaacc ctcaactttc
     4981 gagacgtact tcgcccctct ggacaagcac ttcttcatct ccgtcgtctc tccgggcgaa
     5041 aacagcttcg ccaccgtcac gaccgacatc acctcgatca aaaaggcgga gcagctcatc
     5101 gaagcgaaga acaaggaact ggagcaggtc gtctacgtag cgtcgcacga ccttcgttcc
     5161 ccgctggtca atgtggacgg atacagtcgc gagctggagt actccgtgga ggatcttctg
     5221 cgcgcgctgg acgacgaatc tctcgctcct ccggaacggg agcgcgcgat acgcgccctg
     5281 ctccccgaca tgaccgactc tctgcgccgt atcagaggaa gcgcgaagca gatggacgcg
     5341 ctccttcgag gcctcttgaa gctttcgcgc ctcgggcggg cggcgctcag cgtcggttcc
     5401 gtcgatatga acgagctgtt ggcggaattg agttcctctc tgcaatttca ggtcagagag
     5461 gccggggcgg cgcttcgcgt cggcaggttg ccctcgtgca gaggcgacgc cgtgcagctc
     5521 actcaggttt tcgcgaacct tctgggcaac gcgctgaagt atcttgatcc ccaacgtccg
     5581 ggggaaatag tcgtcgaggg gcggatcgag caggaccgct ccgtctacct ggtgcgggac
     5641 aacggtatcg ggatcgcgcc gcagcaccag gagaagattt tccagctctt ccacaggttg
     5701 aatccatcgg cgaacgaagg cgaggggctt ggcctgacga tcgtcaggca gatccttgga
     5761 agactggacg gggaggtcgt cgtggaatcg acgcccggaa ctggaagcac gttcacgatc
     5821 acgctgcccg cagcgtcgaa aaagggagaa tgattatgga ggaggaagaa cgcatgcaca
     5881 atgaagtcat catactcata gccgaggacg acgaggggca cgccgggctg atacggaaaa
     5941 atttggcgcg cgcgggaatc tccaaccatg tggaacactt caaggacggg caggagatca
     6001 tcgattttct ctttcgcatc gggaacgggc cgcacaggcg gtcgcacacc ccgtacattc
     6061 tgctgctcga catccggatg cccaagctgg acggcacgga agtgctcaag cgcatcaaag
     6121 aggaccacga actccgtaag atgccggtga ttatgatcac cacgaccgac gatccccggg
     6181 aagtggaacg ctgtcacgaa ctggggtgca gcagctacat cacgaaacct gtggaatacg
     6241 acgccttcgt caacgccgtt cgccagctgg ggctctacct tgcagtcgtg caggtgcctc
     6301 aggtgaacgg cgtgcactga gagcggcgga gacccgaagg atggatggac gcgccgagca
     6361 cggggacgcc ccggcacgaa acgttctcgt cgtagaggac aacgaagact tgcgaagcct
     6421 gatcgtcaga aggcttcaca gagcggggta cgaggcgttc ggcgtgtccc tcggatgcga
     6481 ggctgtggag aaagccttcg agcggcccgg aacggcgctc ctgatcgatc ataatttacc
     6541 cgacatgtcc ggacaggagc tggtcgcgct gctgaaagag cgcggtgcgg cgccgccttt
     6601 tatcatcatg accggacagg gagacgagcg actcgcagtg cagatgatga agctgggcgc
     6661 cttcgattat ctggtgaaga actccgaact gctggataaa ctgccggaga cgctggggcg
     6721 cctcttcgga gagctggaaa cggagcggcg aatccttgaa gaccggagca tcctcctcga
     6781 caacatccgg acgcaggtat ggtacctcac cgacgaccat acctacggcg ccgtcaacaa
     6841 ggcgcacgcg gacttctgcg gcctgcggca ggagatgatg tccttccgcg acatgttcga
     6901 actgttcccg agagaggttg ccgaactgca ccggagcaac gcgatgaagg tcttcggcac
     6961 tgcggagccg cttaccgtcg agctctggct tccgtcctcc actggggagc ttcgtctgct
     7021 ctctatttac aaatcgccca aacttcggga ggacggaagc gtagagtatg tggtctgctc
     7081 cgcggaggat gtaaccgagc gcaaacgggc cggagatgct ctccgcgaga gcgaggaacg
     7141 cttcaggaag ctcttttcca gcgtgcagag cgtctcggtc caggggtacg ccgcggatgg
     7201 aacggtgcgc tattggaaca gcgcgtcgga gcagctctac gggtataccg ccgacgaggc
     7261 catcgggggc aatcttcttg atctgatcat accgcgcggc atgagagaag ctgtccggaa
     7321 cgatatccac gacatggtgg agaaaggaga aggccgggcg gcagagcgac ttgccctaaa
     7381 gaagaaagac ggttcccggg tcgatgttct ttcgagccat gttgtggtgg actatatcga
     7441 cggacggagg gaactcttct gcctggatat cgatatagga gagcagatac gaatggagga
     7501 ggccctccgg gcggccaagg agaacgccga atggctcggg cagcaggccg nnnnnnnnnn
     7561 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     7621 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn gaggaggccc tccgggcggc caaggagaac
     7681 gccgaatggc tcgggcagca ggccgaagcc gccagcaaag cgaagagcgc ttttctggcg
     7741 aacaccagcc atgaactccg tacgcctctc aacggtgcga tcggctttct tgaactgctc
     7801 gcggacacgc ccctcaacga actgcagaag gagtacgtcg gctacatacg gacgtccgcc
     7861 tacacgctcc tcgaagtcat cagcgaagtg ctggacatct cgaaaatcga atccgaccgg
     7921 tttgaccttg agtacgccca gagcgatgtt ttcgaggtta tgcggaaggc tgtggacgcc
     7981 gtccgcggga ctgtcttgcg gaaggaactt cgccttgaac tccgcatcga agaagggacg
     8041 ccgcggtacg cagtcgtcga cccgctccgt ctgaaacagg tgctggtgaa ccttttgggc
     8101 aacgccgtca agtttactga aaaaggattc gtcgagcttg cgcttcagtg cgtcgcgctt
     8161 gatgcaggac gatgcgccta tacgttctcc gtcagggaca ccggcatagg tattgctccg
     8221 gaggtccatc gcaaactctt cgagccgttt ttccaggccg acgcttccaa caccagaaag
     8281 tacggcggcg tcgggctcgg catcactatc tgcgaagcgc tgctgcaaaa gatggacagc
     8341 cgcctccaac tggaaagcgc acccggccag ggaagccgct tcttcttcac cctctgcgca
     8401 cgatgcgacg ccgaaccgga agaaacaacg aacggagagc ggacgaacct gctcccggca
     8461 aaaggggttc ctcttccgct gcgtaccaac cggcaagccg tcgttctcct cgccgaggat
     8521 cagcggctca accgaaggct gctgcgaatc ctcgtctcca agctcgtccc gggggcgcga
     8581 atcatcgagg cgtccgacgg agaagaagcg gtccgcttct tcagaagcga agcgcccgac
     8641 atcgtcttta tggatctgca gatgccgctg aaggacgggt gcgaagcggt agcggaaata
     8701 cgttcgcttg aacgggcctc ctccgggggt acggcgaagg ccggaattcc gattattgca
     8761 gtcaccgccg atgtccagaa ggaaacgcga acgttctgtc tcgagaacgg gatggatgat
     8821 ttcattacca aacctgtgga cgcgtggagt ctcagatgcg ttctggagcg ttgcctcgaa
     8881 agtgtcctcc tctaagcgga gaaaagcgta cgtatctgct gagagtatcc gtgcgttccg
     8941 ggagcatcgg gacgcatttt tttgccaaaa taaaggtcaa taaagatcag tgattacata
     9001 ctcttttttc atgaacgttc tgggcggatg ggctgttttc ctagggagta tgcggcgaag
     9061 ttcgcggttt gtctcgttga aaaaaattag tctttgggat gagtaaagtt tttgttgcgg
     9121 agctcgccat tcaaggagat aataagcaaa gtgaaatact tcatattctt tcaggctgct
     9181 gtgaccgcac tctctcagcg gaagaaattt agcgaggaga tcgcccatgc ccttgcgccg
     9241 actgttcttg ctcttgatga tgcttttgct ggcctttgta cttgtactaa cctctttcac
     9301 ctcgcaacgg acgacgctga aagcgttcga cgacgtggag cgccgtttct tcattcagga
     9361 catggcgagg gttcagaacc gcctggacga ggaactccgg gtgctgcgca actatgcggc
     9421 ggactggggc gcatgggaca gcatgtacgc cttcgtggaa aagggctgca aacaccggtt
     9481 cgacgagatg ttcgacggac aggcgatgct tgccgccggc atggaggcgc tcttgatttt
     9541 cgacaccggg cggcgtctcc tgttctccat cttcgagggg gagtgcgcag cggagcggaa
     9601 gctccccgat cacgtgtcca agggcctgaa ggacgctgtt ccttcgctga tggagcgctc
     9661 atctgacggg gcgttcgccg agatcctgct cctgggcgac gcggcttacc tgttcggagt
     9721 cagcccggtg ctgagaacga acaggacggg gccgtcgcaa ggagtgctcc tcatcgggag
     9781 gtcgctgggg aaacttctgc ccgagatgcg gaagagcctc ggcacgaagt tcgacctcct
     9841 tccgggacac gccgcggatg ttccggaccg ctcggcggga gatgtccgga tcgtctccgg
     9901 agaaggggag ggtgcggccg tatggcaggg gagagggaac gaattctccg tccgcatcga
     9961 tgtccccagg ctcatccgcc agcgggggct ggaagctgtc gaatcgtcgc gtctctggat
    10021 tctgctcacc ggcgcgggga ctctcggcct ggtgctgctt ctcctcgaca gggtgattct
    10081 gcgccgtctg cggaagcttc gcgaggtctc cgagcgaatc gtcgccggag agtcgctcac
    10141 gctgcgggtg gacgaagagg ggcccgaaga gatcttcgcg ctctcgcgct cgttcaacat
    10201 gctgttgcag acgctgcacg atctcgtgat gaaaatcccc gatcctttca tccttgccga
    10261 cgccgacggc gccattacgc tggccaacga cgcggcgcac actgtcctga gctacgggac
    10321 cgaccttaaa ggaacgcctc tctcctccgt tctcgtcaag aaggaggatg acccccgcgc
    10381 cgggaacaat agaacagatg cagaaaagga tacggaggcg aaggacgggc acagcgtctt
    10441 cgaggcgtcg ctcctcagag cggacggttc gctgcttccc gtcgaggtgc accgccggag
    10501 cttcactttc ggcgcgctcc cactgtcgct tcttctggcg cgcgacctga aggagcgcaa
    10561 gcagttcgag gagcgcctcg cacggaaagc gtatttcgac gatcttaccg ggctgcccaa
    10621 ccgtcactcg ctcctcaaag cgatcaaccg cctgttgaac gacggaaccc gaacgcgcat
    10681 gttctcgctc gccgtcatca atatggacca cttcaagctg gtcaacgccc aagtcggcaa
    10741 cctgaacggc gaccggattc tcctgctgat cgccgagaag atcaaggaac ttcaggagcc
    10801 cgaggcacag ctcttcagaa cgagcggcga cgaattcgcg ctgctcttcc cccttcccga
    10861 gggcggcgaa agcgaggaga tgctcctgat ggaccgtatc cgcaagggca tagcgacgcc
    10921 gtgcgaggtg ggggacgaga ccgtctttcc ctcggccagt atcggagtgc tcgtcgatat
    10981 tgcggagggg aagaacgcct ccggggcgat cgacgccgca ctgcacgccg tgacgcaggc
    11041 caggaagaac ggcatcggcc ggatcgcccg ctacgttgcg ccggaagtca gagagtacac
    11101 gcgcggtccg aacttgctca cgctccaggc ggaattgcag gtcgccctgg agcgtcacga
    11161 gttcgttccc tacttccagc ccgtctacga catcgtttcc gagaaaat
//
LOCUS       DAOTIL010000006        11186 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminobacteriaceae bacterium isolate AMR_MDS_3026
            NODE_6930_length_11186_cov_3.763813, whole genome shotgun sequence.
ACCESSION   DAOTIL010000006 DAOTIL010000000
VERSION     DAOTIL010000006.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868050
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminobacteriaceae bacterium (wastewater metagenome)
  ORGANISM  Aminobacteriaceae bacterium
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae.
REFERENCE   1  (bases 1 to 11186)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11186)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:19:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,224
            CDSs (total)                      :: 3,198
            Genes (coding)                    :: 3,184
            CDSs (with protein)               :: 3,184
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11186
                     /organism="Aminobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6930_length_11186_cov_3.763813"
                     /isolate="AMR_MDS_3026"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:3034609"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..928
                     /locus_tag="P5201_00210"
     CDS             <1..928
                     /locus_tag="P5201_00210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006299985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="YidE/YbjL duplication"
                     /protein_id="HRV96985.1"
                     /translation="TARVVKRYGLKFILLSVAVTGAGALATLAAGILFSGSASPNMIA
                     GTYTGALTSSPGLGAALEATGGSPDVTIGYSVAYPFGVMAVVLFVQLIPALFGIDVAK
                     EREKLALERKAAEPASPDARPASIPFSLLSFVVCIIGGSVLGGISLPLGPLGSVGLGT
                     TGGALLFALGVGSFDHIGPLQMRMEKNVLSAVRAISLGFFLAVVGLNAGGGVVKAFLE
                     HGVLLIAVGIGSALAAEMVGFFLGHYVWKINWIILAGAICGAMTSTPGLGAAIDATGT
                     DECSAGYGGAYPVAIVCMVLFTTMLAAFFKTR"
     gene            979..2565
                     /locus_tag="P5201_00215"
     CDS             979..2565
                     /locus_tag="P5201_00215"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4118 domain-containing protein"
                     /protein_id="HRV96986.1"
                     /translation="MFTTIRRWAEAHPFFFAPGFVGLATLLFLPGRELLGKGHWALLY
                     LLVISVVASVVGTRPSFVAAVTSFLTWNVFFLPPYNTFRVDDPKDWISLGVFLIVGLL
                     IGLTSGRLRERETTAISQEREMALLNRLTGRLLAIEGALEMAETLCSEIEAVFSPAFI
                     RFHLIAGGEKRKLLFGEKNELSMDPLEEDFLDWVVENRKGVASPASDVARLWSSGGSD
                     SPVLHSSIFSAVSRGDVFLPALSSSGIEGILHVGERRSRSSYRWNEVQLLFAAARMAG
                     TFLERSRLGESARQARTLKEGDKLKSAIFSSVSHELKTPLSSLTATISGLLEQDAEPD
                     PSRVRQELLSVKKDLDRLSHSIGSLLELSRLAAEAWKPDLQWAEPGEVLSWVLFRLDK
                     TDRERVRVDLPEHLPLILVDFQQWGRLLMHLLENALSYSPAGSPVAVGARSTDEGFVT
                     WIADEGPGIPDEEKSRVFEKFFRGTFSNRLPGGTGLGLPIAMEIARVHEGSISIEDNT
                     PAGTRIVVRLPAAHVMETEE"
     gene            2570..3271
                     /locus_tag="P5201_00220"
     CDS             2570..3271
                     /locus_tag="P5201_00220"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HRV96987.1"
                     /translation="MTSAPERNIRVLVIDDEESIRRALKSVLSARKYEVSLAATAAEG
                     IDTAIETNPELVVLDLALPDMDGQDVCKELRAWMTAPILILSVRGNETDKIQALDAGA
                     DDYLTKPFQAGELLARMRALLRRAESRVVSPPELCAGDLTVNLARRQVFVKGEEVSLT
                     RTEFEILALLVQNADCVVTHRMLMEKLWDDKEERDPRLLRVHVSNLRKKIEPAPSLPR
                     FIHTEPGVGFRFTPE"
     gene            3392..3874
                     /locus_tag="P5201_00225"
     CDS             3392..3874
                     /locus_tag="P5201_00225"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRV96988.1"
                     /translation="MTAQDIGLCSVIEKLRRNGAEADIAEAAQYMHRNEITLLMLKRA
                     GVPLEIMDACFLLSQCADSLIPERVEELRRSSLARTIKIGELRALLQEGDMRAAFRDR
                     WEICRKSLVHALDILENGARADESPKKKPTMALFWERFAEFYEETEEMQNVALSGRSS
                     "
     gene            3846..4034
                     /locus_tag="P5201_00230"
     CDS             3846..4034
                     /locus_tag="P5201_00230"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRV96989.1"
                     /translation="MLLCLGDLLEANASCSLEENGELHLYFQDESDILYGEDNKVVVS
                     AGESDLSGSDAVKWLQKH"
     gene            4168..5436
                     /locus_tag="P5201_00235"
     CDS             4168..5436
                     /locus_tag="P5201_00235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009201218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="potassium transporter TrkG"
                     /protein_id="HRV96990.1"
                     /translation="MLLIGAGTLLLLLFNIHSHPLRFVDALFTATSAVCVTGLVVVDT
                     GKDLAPASQTVVLLLIQLGGIGVMTATTALSLLIRERIGLRQRMFLAGGFGMETPAGV
                     VRFLRRILLLTFSVETVGALFLYAGFLRAGFSPLRSLYNAVFHSVSAFCNAGFSLFSD
                     SLAGFSRNLLIPGTVMALIVVGGLGFMVLSELMMTVRRREKLSMLPRLVLFTTAGLLL
                     FGLFSITLSEWNHAFAGMSGGWKIWNGLFASVTPRTAGFDTVPPATFSSAGIFLLILL
                     MIVGASPGSTGGGFKTTTLAVLLVSTWNNLRKNPEINLWNRRISIRILQRTVTVVVLY
                     LGTIGLGLGALALLEPFPFRSLLFETVSAMGTVGLSLGITAELSDAGKIVLMLLMFWG
                     RVGIVTFLYGLLKPVPRGKVSYPDADIPIG"
     gene            5449..6108
                     /locus_tag="P5201_00240"
     CDS             5449..6108
                     /locus_tag="P5201_00240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005661869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TrkA family potassium uptake protein"
                     /protein_id="HRV96991.1"
                     /translation="MNDKEKVVLVAGLGRFGQSLCKTLSEQGRQVIAVDRSRQFVEEM
                     ADIVDLAVQADVTDSEALVKVGAKEADIGIVAIGGNIEASILATTILKDLEIPYVIAR
                     AQNTVHARVLARVGANRVVFLERDMGERLALLLVHPWMSHFVQVPGSRFHVGEIRPLP
                     EMVGKTLPELNFRVRYNAVVLSVNRSGDRFLPTAETVIEAGDMLLVAGRREDLEKWVE
                     E"
     gene            6345..7211
                     /locus_tag="P5201_00245"
     CDS             6345..7211
                     /locus_tag="P5201_00245"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRV96992.1"
                     /translation="MTSTALRPEDTSASAGALLFYYPAAFLLFFADILIVDFPGIRIG
                     MLDVLPAVPGITWSCVGAVFAVLMLVATVRYVAGGGTSDLARLFLRAGHSVVVLLSFI
                     GFLVVPMAVTLSGLSESTDTYGAFVAFRGAFFFLFLFVLSGMSHAFLATGRTDRGTTI
                     VGASQKLLAAQMAMLSAAFLFLVLSAPGTLPSLSCSMDVPSWLVYLLPVCFVLVGALF
                     ASFGGGAEHGALLGFMFRMCVLLSAAAPVAVLLAGRSEESGMALYMASLALFVSALKG
                     LYAVEASEEAAA"
     gene            7369..7965
                     /locus_tag="P5201_00250"
     CDS             7369..7965
                     /locus_tag="P5201_00250"
                     /EC_number="2.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024698.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family protein"
                     /protein_id="HRV96993.1"
                     /translation="MHKQDTPFPVLFTPRLRLCAIESSHTERLHAVWTDPLVIEYLVL
                     DPFTELRQSEEMIGLLQWLRPSGEGVRWAITLRSDGTVLGTCGFHNWKKEHGRAEMGY
                     ELDSRMWRQGFMSEAVFAALEYGFGTMELNRVEALVTEGNQRSRGFLEKAGFRLEGTL
                     RQYEWARGRYQDQWMFSLLKEDRKGGQFTSVIDTPASL"
     gene            8052..9098
                     /locus_tag="P5201_00255"
     CDS             8052..9098
                     /locus_tag="P5201_00255"
                     /inference="COORDINATES: protein motif:HMM:NF012742.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000976 - transcription cis-regulatory
                     region binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HRV96994.1"
                     /translation="MHRTATMKDVARKAGVSTATVSHVLNGTRPVGSVLRDRVRQAVA
                     ELGYRPNFIARSLRRKGSGAVGVILIDDTNPFFSESTRGVKDAALENGYSVLVCNSEG
                     DPDRENEFLELLAEKQVEGVILFNVRDSNLREAAGRRLAVPVVLVDRDGASRGMDSVV
                     VDHYAGGMDAMEHLLSLGHGRIACIAGPSNSTPALLRLRAYANALRRAGIPYDKSLVY
                     RGDFRSESGYAAIPFLFSDGKLPTALFAFNDLMAFGAITALAERGLRIPEDVSVVGYD
                     DILLSSFFNPPLTTVAQPKKEMGRAAFGLLLDRINDESLPARSLRMNARLVVRKSTAS
                     PGASKGGDGSGCMR"
     gene            9238..10275
                     /locus_tag="P5201_00260"
     CDS             9238..10275
                     /locus_tag="P5201_00260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012869312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BMP family ABC transporter substrate-binding
                     protein"
                     /protein_id="HRV96995.1"
                     /translation="MKGLRKVLLLVMVLVIALSAFGVAGAAESKVKVALVLSGFLGDK
                     SFNDSAYEGLERSIKEFGIDLKVLESKVPSDWESNLVAMAAEGYDLVLGCSTQLQEII
                     AKHAPDFPDVKFGIIDGVVKAPNVMSAIFAQNEGSFLAGAAAAMFTTKTDVPGVNAEK
                     IIGWVGGMDIPVLHDFLVGYKQGAAYVDPETRVLVSFAGTFNDPLKGKELTLAQFEQG
                     ADIVMNVASNTGNGILEAAYEAKKYAIGVDLNQDETYPGHILTSMLKRVDMGSYLIVK
                     AVVEGNFEGGKILDMTIANGGVGLTDMSTMKKALGDKFPQDILESIAKLTEEVKGGKI
                     KVDSYEGFQRN"
     gene            10338..>11186
                     /locus_tag="P5201_00265"
     CDS             10338..>11186
                     /locus_tag="P5201_00265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006301377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HRV96996.1"
                     /translation="MSRILIEMKGICKEFPGVKAVQDGLFSLEEGEIHSLIGENGAGK
                     STLMKILYGMYVPEEGSIAVKGETYPSLTPKQAIGLGIGMVHQEFMLVREMTVLENII
                     LGFEPRRGGRIDRRAALEKIEYYADTYGLRVHPDKKVEEISVGEAQRVEIIKTLYRGA
                     EILVLDEPTAVLTPQEAEQLFCILRSMKEGGKSIVFISHKLQEVLAISDRITVMRGGK
                     HIATVAGSSTSAPELARLMVGREVFLGGSPVPAAKGDVALSVENIFVPGEKELSKLRG
                     VSFSVRK"
BASE COUNT         2029 a         3251 c         3519 g         2387 t
ORIGIN      
        1 taccgcgcgg gtcgtcaagc gctacgggct caagttcatc ctgctctccg tcgccgtcac
       61 gggcgcgggg gcgctcgcca cgctcgccgc cggaattctc ttctccggct cggcgtcgcc
      121 gaacatgatc gcggggacgt acaccggcgc gctcaccagt tcgccggggc tcggcgcggc
      181 gcttgaagcc accgggggaa gcccggacgt caccatcggc tactcggtcg cgtacccctt
      241 cggcgtcatg gcggtggtgc tcttcgtaca gcttatcccc gccctcttcg gtatcgacgt
      301 ggcgaaggag cgggaaaagc tcgcgcttga gcgaaaggcg gcggagccgg cctccccgga
      361 cgcgcgcccg gcttcgatcc cgttctcgct gctctccttc gtggtctgca tcatcggcgg
      421 ctccgtattg ggcgggattt cgcttccgtt ggggccgctc ggctccgtcg ggcttgggac
      481 gacgggcggc gcgctgctct tcgccctcgg cgtcggcagc ttcgaccata tcggtccgct
      541 acagatgcgg atggagaaaa acgtcctctc ggcggtgcgc gccatctcgc tcggcttctt
      601 tctcgcggtg gtggggctga atgctggggg cggcgtggtg aaggcctttc ttgagcacgg
      661 ggttctgctc atcgccgtcg gtatcggctc ggcgctggcg gcggagatgg ttggtttctt
      721 ccttgggcac tacgtctgga agatcaactg gatcatcctc gccggggcga tctgcggcgc
      781 gatgacgagc acccccggcc tgggcgccgc catcgacgcg acggggacgg atgagtgcag
      841 cgcggggtac ggcggagcct atcccgtggc catcgtctgc atggtgcttt tcaccacgat
      901 gctcgcggcc ttcttcaaga cgcggtagag gaagcaatcg gtttcccgcc gcttcaagcg
      961 gtcgataagg agaaagatat gttcacgacg atccggagat gggccgaggc gcatccgttc
     1021 tttttcgccc cgggatttgt ggggcttgca acgctgctgt tcctgccggg gagagagctt
     1081 ctcggcaagg ggcactgggc gcttttgtat cttctggtca tttctgtagt ggcctccgtc
     1141 gtcgggacga ggccgtcctt cgtggcggca gtgacgtcgt ttcttacatg gaacgtcttt
     1201 tttctccctc cgtacaacac gttccgggtg gacgatccga aagactggat ttctctcggg
     1261 gtttttctga tcgtcggtct cctgatcggc ctgacctcgg gacgccttcg ggagcgcgaa
     1321 acgacggcca tctcccagga gcgggagatg gcgctcctca acaggctgac cgggaggctg
     1381 ctcgccatcg aaggcgccct tgagatggcg gagacgctct gctccgagat agaagctgtt
     1441 ttctctcctg cctttatccg tttccacctg atcgcaggcg gtgagaaacg aaagcttctg
     1501 ttcggagaga agaacgagct ttcgatggac cctctcgagg aggattttct cgactgggtg
     1561 gtcgaaaacc ggaaaggggt ggcgtctccc gcgtcggatg tcgcccgctt gtggagttcg
     1621 ggcgggagcg acagcccggt gttgcattcg tcgattttct ccgccgtgtc gagaggggat
     1681 gtctttctcc cggcgctctc ttcttcagga atcgagggca tacttcacgt cggggaacgt
     1741 cgcagccgga gttcgtaccg ctggaatgaa gtgcagcttc tcttcgccgc cgcgcgcatg
     1801 gccgggacgt tccttgaacg gagccgcctc ggcgagtcgg cgcggcaggc ccggaccctg
     1861 aaggagggag acaaattgaa gtcggcgata ttttcgtcgg tttcgcacga gttgaagacg
     1921 ccgctctcct cgctgacggc cacgatctcg ggacttctgg agcaggatgc cgaaccggac
     1981 ccttcgcgcg tacgccagga gctgctctcg gtcaaaaagg atctcgacag gctctcgcac
     2041 tccataggct ccctgctgga actttctcgt cttgccgccg aggcgtggaa gccggatctg
     2101 caatgggcgg agcccggcga ggtgctgagc tgggtgctct tcagactcga caagacggat
     2161 cgcgagcgcg tacgggtcga cctcccggaa catttgccgc ttatccttgt cgattttcag
     2221 cagtggggaa ggctgctgat gcacctgctg gagaacgcgc tctcctacag tcccgccgga
     2281 agtcccgtgg ccgtgggcgc ccgcagcacg gacgaagggt tcgtcacgtg gattgcggac
     2341 gagggacccg gaataccgga cgaggagaag agccgggtct tcgagaagtt cttccgggga
     2401 acgttttcga accgccttcc cggcggaacg gggctcggtc tccctatagc gatggagatt
     2461 gccagggtcc acgaaggaag catcagtatc gaggacaata cgcccgcggg aacccgtatc
     2521 gtcgtccggc tccccgcggc gcacgttatg gaaacggagg aatgaacgga tgacgagcgc
     2581 gcccgaaaga aatatcaggg ttctggtgat cgacgatgaa gagtccatca ggcgggcgct
     2641 caagtcggtt ctctccgcca gaaagtacga ggtgagcctc gcggcaaccg ccgccgaagg
     2701 gatcgacacg gccatcgaga cgaaccccga gctggtcgtc ctcgacctgg cgctcccgga
     2761 tatggacgga caggatgtat gcaaggagct gcgcgcctgg atgaccgctc ccatcctcat
     2821 actttcagtg cggggaaacg aaaccgacaa gatacaggct ctggacgcgg gcgccgacga
     2881 ctacctgaca aagccgttcc aggccggcga gcttctggcc aggatgcggg cgctgctgcg
     2941 gagggctgag tcgagggtcg tgtcccctcc tgaactctgc gccggagatc tgaccgtgaa
     3001 tctcgccagg aggcaggtct tcgtgaaggg ggaggaggtt tccctgacgc gcacggagtt
     3061 cgaaatactg gcgcttcttg tccagaacgc cgactgcgtt gttacccacc gtatgctgat
     3121 ggagaagctt tgggacgaca aagaagagcg cgatccgagg cttcttcgtg tgcatgtgag
     3181 caacctgaga aaaaagatcg agcccgctcc ctccttgccg cgcttcattc acaccgagcc
     3241 cggagtagga ttccgcttca cccccgaatg atcttcaccc tttacgaaaa ctttacgccc
     3301 gcgccccgtt tcttgacgga gcctttatcc ctcgcttggt agcatatgcc tgcacatgga
     3361 aagaaatgcc ttttaaacag gaggattctt tgtgactgct caagacatcg ggttgtgctc
     3421 cgtgatagag aagttgcggc ggaacggagc ggaggcggat atcgccgagg ccgcgcagta
     3481 catgcatcgg aacgagatca ccctgctcat gctgaagaga gcgggagtgc cgctcgaaat
     3541 catggatgca tgttttctgc tgagtcaatg cgcggattcg ctgattccgg agcgggtcga
     3601 agagttacgc cgcagctccc tggcacgaac aatcaagatc ggagaactcc gagccttgct
     3661 tcaagaaggg gatatgcgcg ctgccttccg ggatcggtgg gaaatatgca ggaaatcact
     3721 cgtgcatgcg ctggatatac tggagaacgg cgccagggcg gacgaatccc cgaagaaaaa
     3781 acccacgatg gcgctgtttt gggagcggtt cgcggagttc tacgaggaaa cggaggagat
     3841 gcagaatgtt gctctgtctg ggcgatcttc ttgaggcgaa cgcctcgtgt tcgctcgagg
     3901 aaaacggcga actgcatttg tactttcagg atgagtccga tatactgtac ggcgaggaca
     3961 acaaggtggt cgtatccgcc ggggagagcg atctttccgg gagcgacgcc gtgaagtggt
     4021 tgcaaaaaca ctgattttgc gcgtgcataa gaaagaaacc gcgaagagag aaatgcccgg
     4081 gaagacgcac gtccgggagg aggtcgtagt atagcattca gagcctttac ctcgctggcc
     4141 attgaaagga aactcgtcgc agggttcctg ctcctgattg gtgcaggaac tctgttattg
     4201 cttcttttca atatacattc gcatcctctg cgatttgtgg acgcgctctt tacagctact
     4261 tcggcggtct gcgtgacggg actcgtcgtc gtggatacgg gcaaggactt ggcgccggcc
     4321 tctcagaccg tcgtcctgct gctcatacag cttggcggta tcggtgtgat gacggcgaca
     4381 acggcgctct ccctgctgat ccgggagaga atcggactac gacagcggat gttcctcgcc
     4441 ggtggttttg gtatggagac gcctgcgggc gtggtgcgtt tcctgcggcg gattttgctg
     4501 ctaaccttct ccgtcgagac tgtcggggcg ctctttctct acgcggggtt tctccgtgcc
     4561 ggattttccc cgttgcgttc gctgtacaac gccgtgtttc acagtgtcag tgccttctgc
     4621 aacgccggct tctccctctt ctccgacagt ctggccggtt tttcgcggaa tcttctgatt
     4681 ccggggacgg tcatggcgct tatcgtcgtc ggcggactgg ggttcatggt gctttccgag
     4741 ctcatgatga cagttcgcag aagggaaaaa ctttcgatgc tcccgcgtct tgtgctcttt
     4801 acgacggcgg ggcttttgct tttcggcctt ttctcaatca ccctctcgga gtggaatcat
     4861 gcgttcgcag ggatgtccgg cggatggaag atctggaacg gactgttcgc gagcgtcacg
     4921 ccgaggaccg cgggcttcga tacggtccct ccggcgacct tctcctccgc cggtatcttc
     4981 cttttgatac tgctgatgat cgtcggcgcc tcccccggct ccacaggagg cggattcaag
     5041 acgacgacgc ttgcagtgct gctggtctcg acgtggaaca acctcaggaa aaaccccgag
     5101 atcaacctgt ggaacagacg aatctccatc cggatactgc agaggaccgt cacggtcgtc
     5161 gtcctctatc tggggaccat cggattgggc ctgggggcgc ttgcgctgct cgaacccttt
     5221 cccttcaggt cgctgctctt cgagacagtc tcggcgatgg gaacggtcgg cctttccctt
     5281 ggaatcactg cggagctgag cgacgcgggc aagatcgtgc tcatgctcct gatgttctgg
     5341 gggcgcgtcg gcatcgttac cttcctctac gggcttctga aacccgtgcc gagaggaaag
     5401 gtatcctacc ccgatgcgga cataccaata ggttaggagg tgcgggaagt gaacgataaa
     5461 gagaaagtcg tcctggtcgc agggctggga cgttttggtc agtcgctctg caagacgctt
     5521 tccgagcagg ggagacaggt gatcgcggtg gaccgttccc ggcagttcgt cgaggagatg
     5581 gcggacatcg tcgaccttgc cgtgcaggcg gacgttacgg attccgaggc gttggtgaag
     5641 gtcggcgcga aggaggctga catcggcatt gtggcgatcg gcggcaacat cgaggccagt
     5701 atcctcgcga cgaccatact gaaggatctg gagatcccgt acgtcatcgc ccgggcgcag
     5761 aatacagtgc atgcgcgtgt tttggcccgc gtgggggcga accgcgtcgt cttcctcgag
     5821 cgggatatgg gggagcggct ggcgttgctg ctggtgcacc cgtggatgtc gcacttcgta
     5881 caggtgccgg ggtcgcgttt tcacgtcgga gagatccgcc ctcttcccga gatggtggga
     5941 aagaccctgc cggaactcaa cttcagagtg cgctataatg cggtggtatt gagcgtcaac
     6001 cggtccggcg accgcttcct ccccactgcg gaaacagtta tcgaggcggg cgatatgctt
     6061 ttggtcgccg gccggcgtga agatctggag aagtgggtcg aagagtgaag cgccctttcc
     6121 tcctgttcat cgccctaccc tgacgggcag ttttccgcgc acccggaatg ctgcggagcg
     6181 aacgcctgct ctccggaaga ccgcttccgg caagaaggcc attcacgaaa caaataatgg
     6241 agcgccttct tgtaccgatg ttgattttta agcttttttc tgtataatca gagcggcagt
     6301 aaatgccaag gccacactta tttttatttc gaggaggttc tccaatgaca agcacagcac
     6361 ttcgtccaga agacacgtcc gcttcagccg gcgcactcct cttctactat ccggcagcct
     6421 ttctcctttt cttcgccgat attctgatcg tcgatttccc gggaatccgc atcggcatgc
     6481 tcgacgttct tccggcagtt cccggtatta cgtggtcgtg cgtcggcgcg gtcttcgccg
     6541 ttctcatgct cgttgcgacg gtgcgctacg tagcgggagg cggaacgtcg gatctggcgc
     6601 ggctcttcct tcgggccgga cactccgtcg tcgtcctgct ttcgttcatc ggtttccttg
     6661 ttgtgccgat ggccgtcaca ctttccggac tctccgaaag cactgacacg tacggcgcgt
     6721 tcgtcgcgtt ccgcggggcc tttttcttcc tgttcctttt tgtcctctcc gggatgagcc
     6781 atgcgttcct ggcaaccgga cggaccgata gaggcacgac gattgtcggc gcaagtcaaa
     6841 aactcctggc tgcgcagatg gccatgcttt cggcagcctt ccttttcctt gttctgagcg
     6901 ctcccgggac gctgccctct ctctcctgct ccatggatgt gccttcatgg cttgtctacc
     6961 ttcttcctgt ctgctttgtc ctggtcgggg cacttttcgc atcgttcggg ggaggagccg
     7021 agcacggagc gcttcttggc ttcatgttcc gcatgtgcgt acttctctcc gcggccgctc
     7081 ctgtagctgt actgctggcc ggaaggtctg aagagtcggg gatggcgctc tacatggcgt
     7141 cgctcgcgct ctttgtatcg gcgctcaaag gactctatgc cgtggaggcc tccgaagagg
     7201 ctgccgcata gcaaaagctt tctgctccag tcataagggc ggaccgggtt ttttccggtt
     7261 cgcccttttc ccttttctct acgccgtttt gagtgtgtct cgcatcaagg actttcttta
     7321 ccagctgctc gtccgttgat gtgcgggcgc gatatggagg aaaacgcaat gcacaaacag
     7381 gatacccctt tccccgtgct tttcacgccc cggctgcgac tgtgcgcgat cgaatcgtcc
     7441 cataccgagc ggctgcacgc agtctggacc gatccgctcg tcatagaata cctcgttctg
     7501 gatcctttta ccgagctgcg tcagagcgag gagatgattg gccttctcca gtggctgcgc
     7561 ccctccggag agggcgtccg ctgggcgatt acgctgcgct ccgatggaac ggtgctcgga
     7621 acgtgcggct tccacaactg gaagaaggaa catgggcgcg cagagatggg gtacgaactc
     7681 gactcccgta tgtggcggca gggcttcatg agcgaagccg tcttcgccgc gctggagtac
     7741 ggtttcggca cgatggagtt gaaccgtgtc gaggcgctgg tgacggaggg aaatcagcga
     7801 tcgagagggt tcctggaaaa ggcgggcttc aggcttgaag gcacgctgcg ccagtacgaa
     7861 tgggcgcgcg ggcggtacca ggatcagtgg atgttctcgc tcctgaaaga agatcgtaag
     7921 gggggacagt tcacttccgt cattgacaca cccgcctctt tatgagataa aagaatccgg
     7981 ttaatcgttt acgtaatcgt ttaccgcagt gtccctttgc agggaacccc atttttcggg
     8041 aggcgtgtgg tatgcaccgg acggcgacaa tgaaggacgt agcccgcaag gcgggcgttt
     8101 ccaccgcgac ggtttctcac gtgctcaacg gaaccaggcc tgtgggaagc gtcctccgcg
     8161 accgcgtacg ccaagcggtg gcggaactcg gctaccgtcc gaacttcatc gcccgcagcc
     8221 tccgccgcaa aggctccggc gccgtcggcg tcatcctcat cgacgatacc aacccttttt
     8281 tctccgaatc cacccgaggc gtcaaggacg ccgccctcga aaacggatac agcgttctcg
     8341 tctgtaactc ggagggagac cccgaccggg agaacgagtt tcttgagctc cttgcggaga
     8401 agcaagtgga aggggtcatt ctcttcaatg ttcgcgactc caacctgcgg gaggcggcag
     8461 ggcggcggct cgccgtgcca gttgtccttg ttgatcgcga cggcgcctct agaggaatgg
     8521 actcggtcgt cgtggatcac tacgccgggg gcatggacgc gatggagcac cttctttcgc
     8581 tcggtcacgg acggatcgcc tgcatcgcag gcccctcgaa ctccacccct gcgctgctgc
     8641 gccttcgggc atacgcgaac gccctgcgcc gcgccggaat accctacgac aaatccctcg
     8701 tctaccgggg cgacttccgc tccgaaagcg gctacgccgc cataccgttc ctcttctcag
     8761 atggtaagct ccccacggca cttttcgctt tcaacgacct gatggccttc ggcgcgatca
     8821 ccgccctcgc ggagaggggg cttcgcatcc cggaggacgt ctccgtcgtc ggttacgacg
     8881 acatactgct ctcttccttc tttaatccgc cgctgacgac cgtggcgcag ccgaagaagg
     8941 agatggggag ggccgctttc ggtcttctcc tcgacagaat caacgacgag tcgctgcccg
     9001 cgcgctctct gcgtatgaac gcgcggctcg tggtgcgaaa atccaccgct tcgccgggtg
     9061 cgtcgaaagg aggggatggg tcgggctgta tgcgatgaac cgctccgggg gagggttgct
     9121 tttcccgcgg ggcgcgatgc ggcacggtgc gctcctggtt ttccggacga attccgtccg
     9181 gtcggtccgt ccttcgaggg gcggaaacat actattcaca ttcagggagg gaaacgcatg
     9241 aaaggtttga gaaaagttct tctgctggtt atggttctgg ttattgcgct ctccgcgttc
     9301 ggcgtcgccg gagcggccga gtcgaaagtc aaggttgccc tggtcctctc cggcttcctg
     9361 ggcgacaagt ccttcaacga ttccgcgtac gaggggctcg agcgctcgat caaggagttc
     9421 ggcatcgatc tgaaggtcct tgagtccaag gtcccctccg actgggagtc caacctcgtc
     9481 gccatggcgg ccgaggggta cgacctggtt ctgggttgca gcacgcagct tcaggagatc
     9541 atcgccaagc atgctcccga cttccccgat gtgaagttcg gcatcatcga cggcgtggtg
     9601 aaggctccga atgtcatgtc cgccatcttc gcccagaacg agggctcctt cctcgccgga
     9661 gctgccgcgg ccatgtttac aacaaagact gacgttcccg gcgtcaacgc cgagaagatt
     9721 atcggctggg tcggcggcat ggacattccg gtgcttcacg acttcctcgt gggctataag
     9781 cagggcgctg cctacgtcga tcccgagacc cgcgtgctcg tctccttcgc cggaacgttc
     9841 aacgaccccc tgaagggcaa ggagctgacg ctggcccagt tcgagcaggg cgcggacatc
     9901 gtcatgaacg tcgcgtcgaa caccggcaac ggcatcctcg aggcggccta cgaggcgaag
     9961 aagtacgcca tcggcgtcga cctgaaccag gacgagacct atcccggaca tatcctcacc
    10021 tccatgctca agagagtgga catgggcagc tacctgatcg tgaaagcggt cgtcgagggc
    10081 aacttcgagg gcggcaagat tctcgacatg accatcgcca acggcggcgt gggactcacc
    10141 gacatgtcca cgatgaagaa ggctctgggc gacaagttcc cgcaggacat cctggagagt
    10201 atcgcgaagc tgaccgaaga agtcaagggc ggcaagatca aggtcgactc ctacgaggga
    10261 ttccagagaa attagacatc attttccgga ggcggcgggg agatttcccc gtccgtctcc
    10321 attgcgaggt ggggttcgtg tcgcgtatcc tgatcgaaat gaaaggcatc tgcaaggaat
    10381 tccccggagt gaaggcggtc caggacggac tgttctcgct tgaggaggga gagatccact
    10441 cgctcatagg agagaacgga gcggggaagt cgacgctcat gaagattctc tacggcatgt
    10501 acgttcccga ggaggggagc atcgccgtca agggagaaac gtatccgtcg ctgacgccga
    10561 aacaggcgat cgggctcggg atcggcatgg tgcaccagga gttcatgctc gtccgcgaga
    10621 tgacggtgct ggagaacatc atcctcggct ttgaaccgag gcggggaggg cgcatcgacc
    10681 ggagggcggc gctcgaaaaa atcgaatact atgcggatac ctacggactc cgcgtgcatc
    10741 cggataaaaa ggtggaggag atctccgtcg gggaggccca gcgcgtcgag atcatcaaga
    10801 cgctctacag gggcgcggag atcctcgtcc tcgacgagcc gaccgccgtg ctcacgccgc
    10861 aggaggccga gcagctcttt tgcattttgc gctcgatgaa agagggcggc aagtccatcg
    10921 tcttcatctc ccacaagttg caggaagtgc tcgcgatcag cgaccggatc accgtgatgc
    10981 ggggaggaaa gcacatcgcg acggtggcgg gatcctctac atccgctccc gagctggcgc
    11041 gcctgatggt ggggcgcgag gtcttcctcg gcggttcgcc cgtcccggcg gcgaaggggg
    11101 atgtcgccct ctcggtggag aacatcttcg ttcccggcga gaaggagctg tccaagctgc
    11161 gcggcgtctc gttctccgtc cggaag
//
LOCUS       DAOTIL010000007         9934 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminobacteriaceae bacterium isolate AMR_MDS_3026
            NODE_8094_length_9934_cov_3.219354, whole genome shotgun sequence.
ACCESSION   DAOTIL010000007 DAOTIL010000000
VERSION     DAOTIL010000007.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868050
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminobacteriaceae bacterium (wastewater metagenome)
  ORGANISM  Aminobacteriaceae bacterium
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae.
REFERENCE   1  (bases 1 to 9934)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9934)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:19:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,224
            CDSs (total)                      :: 3,198
            Genes (coding)                    :: 3,184
            CDSs (with protein)               :: 3,184
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9934
                     /organism="Aminobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8094_length_9934_cov_3.219354"
                     /isolate="AMR_MDS_3026"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:3034609"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            148..915
                     /locus_tag="P5201_00270"
     CDS             148..915
                     /locus_tag="P5201_00270"
                     /EC_number="2.7.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006301214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III pantothenate kinase"
                     /protein_id="HRV96997.1"
                     /translation="MLLVLDVGNTTTVIGIYEGDTLKKHWRLMSERHTSDELGIYLLN
                     LLSISGISLKALEGAIYSSVVPSLDTALSEGLEEYLGVVPVKVTASLDLGMEIAYSPK
                     HEVGADRLVNSVAGKARFGAPLIVVDFGTAITLDILNKEGAYLGGTISPGLVTGMEAL
                     FGRTAKLPQVSLEPPKSVIGNNTIGSIQAGILFGNAGLVDSLVRKTWSELGTKTPVAA
                     TGGHASVVASLSETITHVDPWLTLEGLRLIYERNRAR"
     gene            912..2024
                     /locus_tag="P5201_00275"
     CDS             912..2024
                     /locus_tag="P5201_00275"
                     /EC_number="1.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013380.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0017150 - tRNA dihydrouridine synthase
                     activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA-dihydrouridine synthase family protein"
                     /protein_id="HRV96998.1"
                     /translation="MTLDELPVPPPSVAAHPVEIGGLRLANPLLLAPLAGVTIPALRL
                     LYSKLGAGAVHTEMISCAGLLVSERQSRKRNNTGDEGYEIVSPGSKTERMLAVLEGES
                     PVIAQLFAGDAETMARGAECALGVQSKIVALGINMACPMPKVLKKGAGSKLLERPEVA
                     FDMVRRLVPLGLPVWPKIRKCPPTLPLTTEAFCEGLLEAGAATVTVHGRTPAQRYTGE
                     ADVSLLPRLATLFPGKVCASGDVFSPERAAEYLNGGCAAVMFARGAIADPFLFPRTLA
                     FLGYEVHNKYRNPSPDFQLSLLLDFGENVCAGSGPRIAVLMVKRLLSGMFKGLPGIGE
                     LRRAAAHILSWKELRGILETCEVYFERREEHSGCAS"
     gene            2035..3585
                     /gene="lysS"
                     /locus_tag="P5201_00280"
     CDS             2035..3585
                     /gene="lysS"
                     /locus_tag="P5201_00280"
                     /EC_number="6.1.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015556191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004824 - lysine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006430 - lysyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysine--tRNA ligase"
                     /protein_id="HRV96999.1"
                     /translation="MNTVPGGDESGPGDEIVRQRMDKLERLLSEEGYNPYILEKWERK
                     HQLEYVRRNFDHLEADEIDESVSIVTAGRIMTLRKHGKAMFANLADETETMQLYLQFN
                     AMGDEQYTFAKKWVDTGDWVGIVGYPFRTQRGELTIHVRQCVLLCKALRPLPEKWHGL
                     KDTELRYRQRYTDLIANPDVRETFRKRSKIISSIRKTLEDHGTLEVETPILSVLAGGA
                     NARPFKTFHNALGMEMYLRIATELYLKRLVVGMFGRVYEMGKNFRNEGLDTMHNPEFT
                     AMEVYWAYADYEDMMALTEEIIRKAAAVVRGPDSSGPVRYQDVDLDFDKPFKRGSMLD
                     LVREYTGVDFRTIADDDEARRIGKERGVEIKGTESRFTVLNLMFETFVEEKLIQPTFV
                     TGHPTEISPLAKRDPENPDYTRRFELFICGKEVANAFSELNDPIDQRARFEDQLKKKE
                     AGDDEAHDFDEDFINAIETGLPPTGGLGIGVDRLVMFLTDSRSIRDVILFPTMKPLVS
                     KSKDAPEA"
     gene            3605..5656
                     /gene="ligA"
                     /locus_tag="P5201_00285"
     CDS             3605..5656
                     /gene="ligA"
                     /locus_tag="P5201_00285"
                     /EC_number="6.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006301203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003911 - DNA ligase (NAD+) activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent DNA ligase LigA"
                     /protein_id="HRV97000.1"
                     /translation="MAESTIRRDPEEVRSRVVFLREEILRHEHLYYVLDTPEIPDDQY
                     DGLFAELRRLEEEHPSLRSPDSPTQRVGGKAAEGFAKVRLSVPMLSLDNALDTEELSA
                     FLTRTAPFAGEHGYLCELKIDGLAVSLVYEDGVFAQGTTRGDGRTGEDVTANIRTIRN
                     LPLRLSGAFPPKIEVRGEVLLQRQHFADLNVEREERGEPLFANPRNAAAGSLRQLDPA
                     VVAERRLSIFLYTVVDPVLFDLKTQEEALRKLAVLGLPVQPAWRRCAAPEDVLAFVEE
                     QREGRFSLPYATDGVVVKLNSIAAWDELGATSHAPRWAIAFKYPPEEKSTRLEDIIVS
                     VGRTGALTPVAVLSPVALSGSVVRRASLHNEDELRRKDVRIGDMVRVRKAGEIIPEIL
                     GPVLDARTGEEREFIMPKHCPACGTPAVRLEGEVALRCPNRSSCPAQLKEGLRHFASR
                     SGMDIRGLGDKIVDQLVEKGLLRSLADLYDLNEQTLASLDRMGEKSARNLINALEQSK
                     ERPFSRLLSALGIRFTGSRSAEILAEVFRDLPTLRDASEETLAAVDGVGPVMASAIRS
                     FFEQPENQRLLERLEAAGVRATLPRLEDERENEHIGSRPFAGMRLVFTGEMESMTRSE
                     AEEMAKRRGAVCSSSVSKKTSFVVAGKDAGSKLSKALSLGVKVVDEEEFLEMTRLTDE
                     A"
     gene            5873..6229
                     /gene="gatC"
                     /locus_tag="P5201_00290"
     CDS             5873..6229
                     /gene="gatC"
                     /locus_tag="P5201_00290"
                     /inference="COORDINATES: protein motif:HMM:TIGR00135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030956 - glutamyl-tRNA(Gln)
                     amidotransferase complex [Evidence IEA];
                     GO_function: GO:0050567 - glutaminyl-tRNA synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatC"
                     /protein_id="HRV97001.1"
                     /translation="MTPRDSSACALDTKAVRHVADLARLRVEDEEMDGLVRHFGAILE
                     HFNVLSAAGKDGRLDLDAVDPFLFSDTEIPPAREDVPIVSSVRGDVLGSAPQRKDSFF
                     VVPRILAQHDGEEFSS"
     gene            6226..7794
                     /gene="gatA"
                     /locus_tag="P5201_00295"
     CDS             6226..7794
                     /gene="gatA"
                     /locus_tag="P5201_00295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005658588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030956 - glutamyl-tRNA(Gln)
                     amidotransferase complex [Evidence IEA];
                     GO_function: GO:0050567 - glutaminyl-tRNA synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatA"
                     /protein_id="HRV97002.1"
                     /translation="MSLCSLSAVEIARGIRNRDFSALEILRSFYQRAETLEPSLHALL
                     HCAPEIAEKHAADVDAAVARGEDPGPLAGVPVVLKDNMCVRGMPATCASRILQGWIPP
                     YDATVTELLETSGAVVAAKGNMDEFAMGSSTEFSAFGPVSNPWDTERVPGGSSGGSAV
                     SVAAGYTPLALGSDTGGSIRQPASFCGIYGLKPTYGLVSRWGLVAFASSLDQIGPFAR
                     TVEDLALSLSVIARPDPRDATSSRRERPDYFEALAPWKTPGRENAPLSGKRIAVVKEL
                     QNRKDAVSAEVEDVLQKTLALCREEGAEIVEISLPVSLEFGLPCYYILAPAEASSNLA
                     RFDGVRYGLSSDEKSLVNLYFKTRGSGFGSEVKRRILTGTYVLSSGYYDAYYLTAQKV
                     RQLMKREFAAAFAGCDLIALPTAPSPAFRKGELVDDPIQMYLADVFTLPVNLAGLPAL
                     SLNGGFTSGEKPLPVGVQFIAPKWGEPELLSTAAVLEKKLGKAKTAPCDWAETSAERA
                     ANAKTAGSERGGVR"
     gene            7791..9284
                     /gene="gatB"
                     /locus_tag="P5201_00300"
     CDS             7791..9284
                     /gene="gatB"
                     /locus_tag="P5201_00300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015557064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016884 - carbon-nitrogen ligase activity,
                     with glutamine as amido-N-donor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatB"
                     /protein_id="HRV97003.1"
                     /translation="MKYTYQPVIGLEIHVQLATSTKIFCSCSTDYIGAEPNTNVCPVC
                     LGLPGALPVLNGKAVELGIKAAMALSCTVKDKTVFHRKNYFYPDLPKAYQISQYDLPL
                     AEGGELFISGDGDTLKRIRITRLHLEEDAGKLVHSAADGRLEGAEFSFVDYNRGGVPL
                     AEIVSEPDLRSPGEAREYVTSLRRLVRYLGVSDGDMERGSMRVDANISLQKTDAATGE
                     ILYWGERAEIKNMNSLRAIERALEYEIRRQIDVMEAGGVLERETRHWNDGEGTTTSMR
                     SKEAAHDYRYFPDPDLPPIEASPALLDQVSASLPEMPWAREARFRNELGLPEADAALL
                     AETPQLADYFESCVLTGASPARISNWVRTEVLRVLNERNISIDSFSMQPKTLGSLVLL
                     VDEGKLSTTAARETFNAMIEKGLPLEKALEASGGAGGKVSGDALADTIRAVLASTPDV
                     VEEILGGKDDKGKKKKFLTGLVMKETRGQADAAAVWELIEKLLNEKP"
     gene            9438..>9934
                     /locus_tag="P5201_00305"
     CDS             9438..>9934
                     /locus_tag="P5201_00305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006583306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HRV97004.1"
                     /translation="MGTRQPENTRSFAIAAHGGAGKTSLVEAMLFDSGSTSRLGKVED
                     GNTVSDFSQEEQKRQISINTSLITMERNGKTLFVLDTPGFADFVGEMRSAVRVADTLV
                     IAVSGTSGIEVQTDKAWQTGEEFGLPVAFYISKLDRENADFGKVLSDIKAVYSEKAVP
                     VFLPIG"
BASE COUNT         2086 a         2764 c         3017 g         2067 t
ORIGIN      
        1 ggctgtgttt tttctttaaa gggcgcgttg ccgcttgcct ctgttttcca gccttccgag
       61 agcccgcctt ctgagtgttc cttgaagata tttccggtgg tataatactc tgatgtttaa
      121 agccttccgt tcaaggagga agatatcatg ttgcttgttc tcgacgtggg gaacaccacg
      181 acggtcatag gaatctatga aggcgatact ctgaaaaaac actggcgtct tatgtccgaa
      241 cgtcatactt cggatgagtt gggtatttat cttctcaacc tgctttccat ctccggaatt
      301 tcgctgaagg cgctcgaagg cgccatctat tcgagcgtgg tcccctctct cgataccgcg
      361 ctctccgagg ggttggagga gtatcttggc gtcgttcccg taaaagtcac cgcaagcctc
      421 gaccttggga tggagatcgc ctattctccg aagcacgagg tgggagccga ccggctggtg
      481 aattccgtcg cagggaaggc ccgtttcggc gccccgctca ttgtcgtcga tttcggaacg
      541 gctatcacgc ttgatattct caataaagaa ggcgcatatc ttggcggcac catttctcct
      601 gggcttgtta cagggatgga agcgctcttc ggcaggacgg cgaaactgcc gcaggtctcc
      661 cttgaacctc cgaagagcgt catcggaaat aataccatag ggtccataca ggccggtatc
      721 ctgttcggca acgccggact ggtcgactct ctcgtacgga agacatggag cgaattggga
      781 acgaagacgc ccgtggctgc caccggcgga catgcttcgg tcgtcgcgtc gctctcggag
      841 acgatcacac atgtcgatcc gtggctgacg cttgaggggc tgcgcctgat ctacgaaagg
      901 aatcgcgcca ggtgaccctt gacgaattgc ctgttcctcc gccctctgtc gctgcacatc
      961 cggttgagat aggggggctt cggcttgcga acccgctgct tctcgctccc ttggccggag
     1021 tgaccatacc cgcccttcgg cttctctact caaagctcgg cgccggggcg gtgcatacgg
     1081 agatgataag ctgcgccgga ctgctcgtat cggaaagaca gtctcggaag cggaacaaca
     1141 ccggagacga ggggtatgag atcgtctccc ccggatcgaa gactgagcga atgcttgccg
     1201 tcctggaggg agaatcgccc gtaatagccc agctttttgc aggagatgca gaaaccatgg
     1261 ccaggggggc tgagtgtgca ctcggggttc agagtaaaat cgtcgctctg ggcataaata
     1321 tggcctgtcc gatgcccaaa gtactgaaga agggcgccgg ttcgaaattg ctggagcgtc
     1381 ccgaggtggc cttcgacatg gtgaggcggc ttgttcccct cgggcttcct gtctggccga
     1441 agatacgcaa gtgccccccg acgttgccgc tgacgacgga ggcgttctgc gaagggcttc
     1501 tggaagcggg cgccgcgacg gtgaccgttc acggaagaac gcccgcgcaa cgctataccg
     1561 gagaagccga cgtttctttg cttccgcgtc ttgcaacgct ttttccggga aaagtctgtg
     1621 ccagcggaga tgttttctcc cccgaacggg ccgcggaata cctcaacgga gggtgcgccg
     1681 cagtgatgtt tgcacgggga gcgattgcgg atccgtttct tttcccgcga acgctggcgt
     1741 ttctcggcta tgaagtgcat aataaataca gaaatccctc cccggatttc caactttccc
     1801 ttcttctgga tttcggcgaa aacgtctgcg ccggatcggg gcccaggatc gccgttctca
     1861 tggtgaagcg gcttctgtcg ggcatgttca aagggcttcc cggaattggc gaactgcggc
     1921 gcgccgctgc gcacatcctc tcctggaagg aactccgggg aatacttgaa acgtgtgagg
     1981 tttattttga aaggagagag gaacatagtg gatgcgcatc atgagcagga agtcctgaac
     2041 accgttccgg gaggagatga gtccggtccg ggcgatgaaa tagtccgcca gaggatggac
     2101 aagctggagc gcttgctctc cgaagagggg tacaacccgt atatcctgga gaagtgggaa
     2161 cgaaagcacc agttggaata cgtccggcgg aacttcgatc acctcgaagc ggatgagatc
     2221 gacgagtctg tttccatcgt cactgcggga agaatcatga cgcttcggaa gcacggaaag
     2281 gcgatgttcg ccaaccttgc cgacgaaacg gagacgatgc aactctacct ccagttcaac
     2341 gcgatgggag atgaacagta cacctttgcg aaaaagtggg tggacaccgg agattgggtg
     2401 ggcatcgtcg ggtatccctt cagaacgcag cgcggggagc tgacgatcca tgtgcggcag
     2461 tgcgtgctcc tctgcaaggc cctccgcccc cttcccgaaa agtggcacgg cctgaaggat
     2521 acggagctgc gctaccgtca gaggtatacg gatcttatcg cgaaccccga tgtgcgcgag
     2581 acgttccgca aacgctccaa gatcatctcc tcgatccgga agacgcttga agaccacggc
     2641 acgctcgaag tggagacgcc gattctctcg gtccttgcag gtggcgcaaa cgcacgcccg
     2701 ttcaagacgt tccacaacgc acttgggatg gagatgtacc tgcgcatcgc aacggagctc
     2761 tacctgaagc gtcttgtcgt ggggatgttc ggcagagtct acgagatggg gaagaacttc
     2821 cggaacgagg ggctcgacac aatgcacaac cccgagttca ccgcgatgga ggtctactgg
     2881 gcctatgcgg actacgagga tatgatggcg ctcacggagg agatcatcag gaaggccgcg
     2941 gctgtcgttc gcggcccaga ttcctccggt cccgtgcgct accaggatgt ggacctcgat
     3001 ttcgacaaac cgttcaaaag aggctcgatg cttgatctcg tgagggagta caccggagtc
     3061 gacttccgta ccatcgccga cgacgacgag gcccggagga ttgggaaaga gcgcggcgtg
     3121 gagatcaagg gaaccgaaag ccgctttacg gtgctcaacc tgatgttcga gacgttcgtc
     3181 gaagagaagc tcattcagcc gacattcgtg acgggacacc cgacggagat ctcgccgctc
     3241 gcgaagcgcg atccggagaa ccctgactat acccggcgtt tcgagctgtt catctgcggc
     3301 aaggaagtcg ccaatgcatt cagcgagttg aacgatccca tcgaccagag agcgcgcttc
     3361 gaggaccagc tgaagaagaa ggaggccggc gacgacgaag cgcacgactt cgacgaggat
     3421 ttcatcaacg cgatcgagac gggacttcca ccgaccgggg gactgggtat cggcgtcgac
     3481 cgcctggtca tgttccttac ggattcgcgc tccattcgcg acgttattct cttcccgacg
     3541 atgaagccgc tcgtctccaa aagcaaggac gcgccggagg cgtagaaaga gaggcgtcgt
     3601 ccaggtggcc gagagcacga tacgccggga tcctgaagaa gtacgttcgc gggttgtttt
     3661 tttacgagag gaaatcctgc ggcatgagca tctgtactat gtactggaca ctcctgagat
     3721 cccggacgat cagtacgacg ggctcttcgc cgaactgcgg cggctggagg aggaacaccc
     3781 ttctctccgg tcgccggatt ccccgacgca gcgggttgga gggaaggcgg ccgaggggtt
     3841 tgccaaggta cggctctccg ttcctatgct cagtctcgat aatgcgctcg atacggagga
     3901 actctcggcg tttctcaccc gtacagcacc cttcgccggt gaacatggct atctttgcga
     3961 gctgaaaatc gacggccttg ccgtttctct tgtgtatgag gatggcgtct tcgcgcaggg
     4021 aacgacacgc ggggacggga ggacgggcga agatgtcacc gcgaatattc ggacgatacg
     4081 caatctcccg ctgcggctct cgggcgcatt cccgccgaag attgaggtcc gcggcgaggt
     4141 gttgcttcag cggcagcact tcgccgatct gaacgtggag cgggaggaga ggggggagcc
     4201 cctcttcgcc aacccgagga atgcagccgc cggaagtctg cggcaacttg atcccgctgt
     4261 cgtggcggaa cgccgcctct cgattttcct ctacactgtc gtcgaccccg ttctcttcga
     4321 tctcaagacg caggaagagg cccttcgcaa gcttgcagtc cttggacttc cggtgcagcc
     4381 cgcttggagg cgttgcgccg cgccggaaga tgtgctcgca ttcgtcgaag agcagcgtga
     4441 ggggcgcttt tcgctcccct acgcgacgga cggcgtcgtg gtcaagctga actccatcgc
     4501 cgcatgggac gaactcggag caacgtcgca tgcgccccgc tgggcgatcg ccttcaaata
     4561 cccgccggaa gaaaagagta ctcgcctgga ggatatcatc gtctccgtcg gacgcaccgg
     4621 cgcccttact ccggttgcgg tgctctcacc tgttgctctt tcaggctcgg tcgtgcggag
     4681 ggcaagtctc cacaacgaag acgaactccg ccgcaaggat gtacgcatag gcgatatggt
     4741 acgtgtccgc aaggccggcg agatcatccc ggaaatactc ggtccggtgc tcgacgcgcg
     4801 gacgggggag gaacgcgagt tcatcatgcc caagcattgc cccgcatgcg gaacgccggc
     4861 ggtacgactt gaaggagagg ttgcgcttcg ttgtccgaac cgttcctcct gtccggccca
     4921 gctcaaggaa gggctcaggc acttcgcctc gcggagcggg atggatatcc ggggactggg
     4981 agacaagatc gtcgaccagc tcgtcgagaa gggcctgctt cgttcgcttg ccgatcttta
     5041 cgatctgaac gagcagacgc tcgcctcgct tgaccggatg ggggagaagt ccgcacggaa
     5101 ccttatcaac gcgctggaac agtcaaaaga gcgtccgttt tcccgccttc tctccgccct
     5161 cggcattcgt tttaccggtt ctcggagcgc ggagattctt gcggaggtat tccgtgatct
     5221 gccgacgctt cgagacgcat ccgaggagac tcttgccgca gtcgacggcg ttgggccggt
     5281 aatggcgtcg gccattcgat cgttcttcga gcagcctgag aatcagaggc ttcttgagcg
     5341 attagaggcg gccggagtcc gggcgacgct tccccgactt gaagatgagc gggaaaacga
     5401 acatattggc tcccggccgt tcgcgggaat gcgcctggtt tttaccggcg agatggagtc
     5461 catgacgcgc agcgaggcgg aagaaatggc aaaacgtcga ggcgcagttt gttcttcgag
     5521 cgtcagcaag aagacgtctt ttgtcgtggc gggaaaggat gcggggagca agctttcgaa
     5581 ggcgctctct cttggcgtaa aagtagtgga tgaagaagaa tttcttgaga tgacccgcct
     5641 gacggacgaa gcgtaacctt cattcgccgc gggctgcatt tcaagcagat cgaaaggaaa
     5701 tcgggaagac tggtggaata ctatgcaaag cgttcctgaa cgttctcttc gagaaacaag
     5761 gagaacggaa gcttttctct ccgttttttt cggcgtgaga gattcctgaa tccgtttggg
     5821 aaagcgatgc gctctatcgt tcgccgtctg cttatcagga ggtttgaata caatgacacc
     5881 cagagactct agcgcgtgcg cgctggacac aaaggcggtg cggcatgtag ccgatctcgc
     5941 cagactccgc gtggaggacg aagaaatgga tgggcttgtt cggcacttcg gagccatcct
     6001 tgaacatttc aatgtgctct ccgctgccgg gaaagatgga cgtctcgatc tcgacgccgt
     6061 cgacccgttt ctattctcgg atacggagat tcctcctgcg cgggaagatg tcccaatcgt
     6121 ttcgagtgtg cgcggggatg ttcttggctc ggccccgcaa agaaaggact ctttttttgt
     6181 cgtgccgaga attctcgcgc agcacgacgg agaggagttc tcatcatgag tctctgttcg
     6241 ctctccgcag tcgagatcgc caggggaata cggaatcgcg acttttccgc cctggagatt
     6301 ctcaggtcct tttatcagcg tgcagaaacc ctcgaaccct ctttgcatgc acttcttcat
     6361 tgtgccccgg agattgccga aaagcatgcc gccgatgtcg acgctgccgt cgcgcgaggg
     6421 gaagaccccg gtcctcttgc cggagtgccc gttgtgctca aggacaacat gtgcgtccgc
     6481 ggaatgcccg cgacttgcgc aagcaggatt ttgcaggggt ggattcctcc gtacgacgct
     6541 acggtgaccg agctgcttga gacatccgga gctgttgttg ccgcaaaggg caatatggat
     6601 gaattcgcca tggggagttc aacggagttc tcggccttcg ggccggtctc gaacccttgg
     6661 gatacggagc gcgttccggg ggggagctcc ggcggcagcg ccgtctccgt cgccgccgga
     6721 tacaccccgc ttgccctcgg gagcgatacc ggcggttcga tccgccaacc ggcctccttc
     6781 tgcggtattt acggcttgaa accgacgtac gggctcgtaa gccgatgggg ccttgtggcg
     6841 tttgcctctt cgctcgatca gataggtcca ttcgcccgga ccgtagaaga tcttgccctt
     6901 tctctctccg tgattgcgcg ccccgacccc cgcgacgcga cctccagcag aagggagcgt
     6961 cccgactatt ttgaggctct ggcgccttgg aaaactccgg gacgggaaaa cgctcctctc
     7021 tccgggaaga gaatcgccgt agtgaaggaa ctgcaaaaca ggaaagacgc cgtttccgcc
     7081 gaggtggagg acgttctcca aaaaacgctt gcactctgcc gggaggaggg ggccgaaatt
     7141 gttgaaatat cccttcccgt ctcccttgaa ttcggcctgc cgtgctacta catcctcgct
     7201 cctgccgaag cgagttccaa ccttgcccgg ttcgacggcg tccgttacgg cctctcttcg
     7261 gacgaaaaat ccttggtgaa cctctacttt aaaactaggg gctccggttt cggcagcgaa
     7321 gtgaaacgga ggattctcac gggaacgtat gttctcagct cgggatacta cgacgcctac
     7381 tacctgaccg ctcagaaagt caggcaactt atgaaaaggg aattcgcggc ggcattcgcc
     7441 gggtgtgatc tcatcgccct tcccacggct ccttctccgg catttcgaaa gggagagcta
     7501 gtggacgacc ccatccagat gtatttagcg gatgtcttta ctcttcccgt gaacctcgcc
     7561 ggactcccgg cgctctcgtt gaacgggggc ttcacatcgg gtgaaaagcc gctccctgta
     7621 ggggttcagt tcatcgctcc caaatggggg gagccggagc tcctttcgac ggcggcggtg
     7681 cttgagaaga agctcggcaa ggcaaaaacc gctccgtgcg attgggcgga aacctccgcg
     7741 gagagggcgg caaacgccaa aaccgccggt tcggaaaggg ggggcgtcag atgaaatata
     7801 cctaccaacc ggtcatcggt cttgagattc acgtccagct tgcgacatca acgaaaattt
     7861 tctgctcctg ttccacggac tacatcggag cggagccgaa tacgaacgtt tgccccgtct
     7921 gcctcggcct gccgggagcg ctccccgtgc tcaacgggaa ggctgtggaa ctcgggatca
     7981 aggcagccat ggcgctctcc tgtacagtca aggacaagac ggtctttcac agaaagaact
     8041 acttctaccc cgacttgccg aaagcatatc agatcagtca gtatgacctt cctctggcgg
     8101 agggggggga gctcttcatc tccggggacg gcgatacgct gaagcgaata cggatcaccc
     8161 gcctgcatct tgaagaggat gccgggaaac tggtgcattc ggcggccgac ggccgactcg
     8221 aaggggctga gttctctttt gtcgactata acaggggcgg cgttccgctg gctgagattg
     8281 tttcggagcc tgatcttcgt tcgcccggcg aggctcgcga gtacgtgact tcgcttcgca
     8341 ggcttgttcg ttatctcggc gtctccgacg gcgacatgga gcgcggttcc atgcgcgtcg
     8401 acgcgaatat ttcgctgcag aagacagacg ccgcgacagg tgagattctt tactggggag
     8461 agcgcgccga gataaaaaat atgaactcgc ttcgcgcgat agaacgcgcc ctggaatacg
     8521 agattcgcag gcaaatcgac gtcatggaag caggaggcgt tctcgaacgg gaaacgaggc
     8581 actggaacga cggagaagga acgaccacct cgatgcgaag caaggaggcg gcccacgact
     8641 atcgatattt ccctgatccc gatctgcctc ctatcgaagc gtctcccgca ctattggatc
     8701 aggtgagcgc atcccttccc gaaatgccgt gggccagaga ggcccgcttc cgcaacgagt
     8761 tgggccttcc cgaagcggat gccgctctcc tcgcggagac gccgcagctt gcggactact
     8821 tcgagagctg cgtcctgacc ggcgcctctc ccgccaggat ttcgaactgg gttcgtactg
     8881 aagtgctccg tgttctgaac gaacgaaaca tatccatcga ctccttctct atgcagccga
     8941 aaactctcgg ttccctggtg cttctggtgg acgaggggaa actgtcgact actgctgcaa
     9001 gggagacttt caacgcaatg atagaaaaag ggctccctct cgaaaaggcg ctggaggcga
     9061 gcggaggcgc gggagggaag gtctccggag acgcgcttgc cgataccatc cgggctgtgc
     9121 ttgcatcgac tcccgacgtc gtggaggaaa tcctcggagg caaagacgat aagggcaaga
     9181 agaagaaatt tttaaccgga ctcgttatga aggagactcg cgggcaggcg gatgcagccg
     9241 ccgtctggga gttgatcgag aaattgttga atgaaaaacc ctgaaacatt cgtcttctct
     9301 caaggggtgt attttaccag cttttgaaaa gagatttttt tgattcgttg actaatggag
     9361 gcattttgcg taagatattc aagttgccct gcagtagagc agaggcagga acgaaacgcc
     9421 ggaggaggta gttggcaatg ggaacccgtc agcctgagaa cactcgcagt ttcgcaatcg
     9481 cggcccatgg aggcgcagga aagaccagtc tcgttgaggc catgcttttc gatagcggca
     9541 gtacgtccag actgggaaag gtcgaagacg gcaacaccgt cagcgatttc agtcaggagg
     9601 aacaaaagcg gcagatctcg atcaacacat ctcttatcac tatggagagg aatgggaaga
     9661 ccctctttgt cctggatact ccgggatttg ctgatttcgt aggagagatg cgctcggccg
     9721 tacgcgtcgc cgataccctt gtcatcgccg tgagcggaac gagcggcatc gaagtgcaga
     9781 ccgacaaggc ctggcagacg ggagaggagt tcgggcttcc cgtggctttc tacatctcca
     9841 agctggaccg tgagaatgcg gatttcggca aggtgctctc cgatatcaag gcggtgtaca
     9901 gcgagaaagc cgttcccgtc ttccttccga tagg
//
LOCUS       DAOTIL010000008         9350 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminobacteriaceae bacterium isolate AMR_MDS_3026
            NODE_8784_length_9350_cov_3.683593, whole genome shotgun sequence.
ACCESSION   DAOTIL010000008 DAOTIL010000000
VERSION     DAOTIL010000008.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868050
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminobacteriaceae bacterium (wastewater metagenome)
  ORGANISM  Aminobacteriaceae bacterium
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae.
REFERENCE   1  (bases 1 to 9350)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9350)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:19:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,224
            CDSs (total)                      :: 3,198
            Genes (coding)                    :: 3,184
            CDSs (with protein)               :: 3,184
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9350
                     /organism="Aminobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8784_length_9350_cov_3.683593"
                     /isolate="AMR_MDS_3026"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:3034609"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(141..1160)
                     /locus_tag="P5201_00310"
     CDS             complement(141..1160)
                     /locus_tag="P5201_00310"
                     /inference="COORDINATES: protein motif:HMM:NF015983.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HRV97005.1"
                     /translation="MVEVTRRCDLRCPVCFASAGEGEDPPFERMAGIFKKIAARAPGS
                     IVQISGGEPAMRDDLPELVAEAAGMNFAGVQLNTNGLRLASEPGFARTLKEHGLSWVY
                     LQFDGTDDAVYERLRGRPLFDLKRRAVERCAEAQLGVVLVATVRRGTNDHLLGDIIRF
                     GTAHAPTVRGVHFQPMAFFGRYPEAPKDGDRTTLADVLRSVCEQHGFFRMEDFSPCGG
                     LHPLCTFTGEFLVSGGELARKETERVHEELDLVRATSRVASRWTLPSPSGGGEEDGFE
                     KILSWRRRIFTITAMAFQDAWTIDTERLQRCPIKYVAHDGRFFPFCAYNATSTDGRSL
                     YRKDA"
     gene            complement(1167..1424)
                     /locus_tag="P5201_00315"
     CDS             complement(1167..1424)
                     /locus_tag="P5201_00315"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRV97006.1"
                     /translation="MSFPKRTRSLCPVCMKPVDAVYQPEGRDIFLEKQCPEHGRFRTI
                     VWRGPLSLDEWSGGEIPEHPFTPSSRCPLDCGACEAHEAFG"
     gene            complement(1421..1879)
                     /locus_tag="P5201_00320"
     CDS             complement(1421..1879)
                     /locus_tag="P5201_00320"
                     /inference="COORDINATES: protein motif:HMM:NF021252.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C-GCAxxG-C-C family protein"
                     /protein_id="HRV97007.1"
                     /translation="MDVFDLDDLQMEMLSLEGKGYHCSQILILLALRQTGGERNDSLI
                     RSMGGLSLGLGHSNGTCGALLGGACLLGFYAGKGNDGETEHPMFRMMVRRLTEWFRDD
                     LCASGSVLCGDILNEHGRARCPQLVRSVWGKAMELLLENDIDPTEGRPEE"
     gene            complement(1879..2094)
                     /locus_tag="P5201_00325"
     CDS             complement(1879..2094)
                     /locus_tag="P5201_00325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005659563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HRV97008.1"
                     /translation="MKELIKSFEKYTKTECGACGRFLELQKTEITYLGSVFTLDLPVC
                     PSCGTVFVPEELATGKMLEVEKILEDK"
     gene            complement(2091..4397)
                     /locus_tag="P5201_00330"
     CDS             complement(2091..4397)
                     /locus_tag="P5201_00330"
                     /inference="COORDINATES: protein motif:HMM:NF026042.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HRV97009.1"
                     /translation="MEKARLLEFESKCVQEEPPYCQAACPLHLDGRALCAFVREGRID
                     DARKLIERTLPLPEALARICDAPCKEACLRRELGGAIELGALERACVEQGPLRRAPLL
                     IPKNGKRVAVVGGGLSALCAASEGIRKGYSVSVFCAEDRAAALTALSPLITDEIARRE
                     LAWLEKGGVLFLPLEDLPPLSELSVRFDAAYAGLDDPFAACATAEATASVNPVSLETS
                     LRGLFAGGAAPSFILRAADGRRGMKSAERFLQGASLFSSREKEGPYATRLFTSTEGMA
                     SAEPEAVPQEGYSLEEAVREAKRCILCECMECVKHCAYMQHYKGYPKKFAREIYNNLS
                     VIQGTRLANGMINSCTLCGRCERICPNGFSMADLCREARREMLRQEKMPPSAHDFALE
                     DMRFNNSPRATLLRHEPGKETSAYLFLPGCRLAGCTPEQTTALYAFLRDRLEGGVGFW
                     LRCCGAPADWAGRDEELHREAAKITEEWESMGSPVVVAACTSCLQTFRKATPSIKAVS
                     LWEILEGTELPEGALSFERTLAVADPCTAAQAPEIRDAVRNVASRAGVRFEEPLLSGE
                     FSFCCGFGGLQESANPTLAALSAKTRGEESSLDFLVYCAMCRNLFAQAGKPAVHLLDI
                     LFPQGDEDPAGRPAIGYSEQQENRVGLVRALRASLWKEEDIVNEPHESIRLVIPPDVE
                     KTLAKRRILADTVRRAIWSAERSGRKMKQINGALVASLRPASVTYWVEYAPKGDGSFE
                     VRNGWSHRMFVAGAAENLVPDASGGDGA"
     gene            complement(4455..6146)
                     /locus_tag="P5201_00335"
     CDS             complement(4455..6146)
                     /locus_tag="P5201_00335"
                     /inference="COORDINATES: protein motif:HMM:NF014758.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HRV97010.1"
                     /translation="MVTVEDDFYVGRQPHMPVEPDVGFAYTDHEGRLVIHSKSIGVHL
                     HLAMIAPGLGVEPEKLVLVTNPAGGTFGYKFSPTMEALVGAAALATGHPVALKYTWKQ
                     QQQYTGKRSPFFVNMKFAADKEGKLLAMETDFSVDHGPYSEFGDLLTLRGIQFMGAGY
                     DIPNIRGEGRTVCTNHAWGSAFRAYGSPQSLFSSEVLMDELAEKLGMDPLEIRYKNAY
                     RPGSTNPTGQAPESYSLPKMLEALRPKYELAKKRAAEGSTTRFKKGVGLSVGVYGCGL
                     DGPDGSEARLDMNPDGTITVCTAWEDHGQGADAGAIGTAHEALRPLGISPDKLKFTWP
                     NTAKCPNSGPAGGSRSQVMTGNAIRVACETLLRETAKPKTGFLKRDGGFMTYNELVAA
                     GKPTSFTGKWSAVEGTACNEDGQGKPFVIYMYGVFMAEITVDTETGKTSVDRMTLMCD
                     CGKINNRLVVDGQNWGGMAQGIGLALTEDFEDIEKHSTMPGAGFPYIKDIPDDMEIIY
                     FEEHREDGPHGAAGIGELPLSSPHASIINGIYNATGVRITRLPAYPEKVLAGLKK"
     gene            complement(6335..7198)
                     /locus_tag="P5201_00340"
                     /pseudo
     CDS             complement(6335..7198)
                     /locus_tag="P5201_00340"
                     /inference="COORDINATES: protein motif:HMM:NF013482.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="2Fe-2S iron-sulfur cluster-binding protein"
     regulatory      complement(7443..7561)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01055"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="molybdenum cofactor riboswitch; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /bound_moiety="molybdenum cofactor"
                     /db_xref="RFAM:RF01055"
     gene            7919..8173
                     /locus_tag="P5201_00345"
     CDS             7919..8173
                     /locus_tag="P5201_00345"
                     /inference="COORDINATES: protein motif:HMM:NF015947.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB/MazE/SpoVT family DNA-binding
                     domain-containing protein"
                     /protein_id="HRV97011.1"
                     /translation="MEVNVVKVGNSKGIRIPAPLLKQLGIGERVILEVEGDALLVRPV
                     RAPRQGWAAAFGRMRRSGDDVLLMDDALDDDMLEAWDEKR"
     gene            8160..8501
                     /locus_tag="P5201_00350"
     CDS             8160..8501
                     /locus_tag="P5201_00350"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF014505.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004521 - endoribonuclease activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system PemK/MazF family
                     toxin"
                     /protein_id="HRV97012.1"
                     /translation="MKNDSENLVQYGVYWTDLDPTQGAEMNKTRPCVVVSPPEMNMHL
                     LTVLVAPVTSRQRESYPTRVHFSIGDVSGWIVLDQLRAIDRKRLRRKIGCLDGETALL
                     VKNVIREMLVD"
     gene            complement(8725..>9350)
                     /locus_tag="P5201_00355"
     CDS             complement(8725..>9350)
                     /locus_tag="P5201_00355"
                     /inference="COORDINATES: protein motif:HMM:NF014035.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HRV97013.1"
                     /translation="SSMSAIDAIAVFGDLFAVGKFWMHTDKRRLLTLLLFALPGLGVG
                     YMVLRFASDDQLRPIIGGVVLAMLILHQVSRVLKQRRGAAAAADGKTSPLLTAVFGFF
                     AGVGTGMANASGPVMSLYLLIAGLPKLPFIAVTAWFFFIMNLLKVPLFFSLGLITMQS
                     LRINMLALPSIAAGALLGYWIVKRISQERFNQIVLTLAFVAALRLLT"
BASE COUNT         1656 a         2814 c         3025 g         1855 t
ORIGIN      
        1 gaggcgctct ctgtagaacg ggctctccgc gcggacgagc tccaccgtct ggcggaggcg
       61 ctccagctgg tagcgctcga ggcgttcccg gcagaagggc tcccccgtca ttcccgcgat
      121 ccatccgtcg agaaacggtc tcatgcgtcc ttcctataga gcgaacgccc gtccgtcgag
      181 gtggcgttgt acgcgcagaa ggggaagaac cgcccgtcgt gcgcgacgta cttgataggg
      241 cagcgctgga gccgttcggt atcgatggtc catgcgtcct ggaacgccat cgcggtgatg
      301 gtgaatatcc gccgccgcca cgagagtatt ttctcgaaac cgtcctcctc gccgccgccg
      361 gagggggacg gaagcgtcca tcttgaggcg acgcgcgacg tggcgcgcac caggtcgagc
      421 tcttcatgca cgcgctcggt ctccttcctg gcgagttccc cgccggagac cagaaattcg
      481 ccggtgaatg tgcagagcgg gtgcagaccg ccgcaagggg agaagtcctc catgcggaag
      541 aagccgtgct gttcgcacac gctcctcagt acgtcggcga gcgttgttcg gtcgccgtcc
      601 ttcggggcct cggggtatct gccgaagaag gccatcggct gaaagtggac tccgcggacc
      661 gtcggggcgt gcgccgtgcc gaaccggatg atgtcgccga gcaggtggtc gttcgtgccg
      721 cgccgcacag tggcgacgag gacaacgccg agctgcgcct cggcgcatcg ttcgacggcg
      781 cgccgtttca ggtcgaaaag agggcgtccc ctgagtcttt cgtagacggc gtcgtcggtt
      841 ccgtcgaatt gaaggtacac ccacgaaagg ccgtgctcct tcagggtgcg tgcgaagccg
      901 ggttcggagg cgagacggag accgttcgtg ttgagctgga ctcccgcgaa gttcatcccg
      961 gcagcctcgg cgacgagttc ggggaggtcg tccctcatgg cgggctcccc gccggagatc
     1021 tgcacaatgc tcccgggagc gcgcgcggcg atttttttga agatgccggc catgcgctca
     1081 aacggaggat cctctccttc cccggcggag gcgaaacaga cggggcagcg aaggtcgcag
     1141 cgacgtgtga cctcgaccac gaaagatcag ccgaaggcct cgtgcgcctc gcatgcgccg
     1201 cagtcaaggg ggcagcggga tgagggggtg aacgggtgtt ccggaatttc gccgccgctc
     1261 cactcgtcca gggagagcgg tcccctccag acgatggtcc ggaagcggcc gtgttcaggg
     1321 cactgttttt ccaggaaaat atcgcgcccc tccggttggt agacggcgtc gacgggcttc
     1381 atgcagacgg ggcagaggct gcgcgtgcgt ttcgggaagc tcattcctcc ggccgtcctt
     1441 cggtcgggtc gatatcgttc tccaaaagca gctccatcgc cttgccccag acggagcgga
     1501 cgagctgcgg acagcgggcg cgtccgtgtt cgttgagaat gtcgccgcag aggacgcttc
     1561 cggaggcgca caggtcgtcc cggaaccact ccgtcagtcg gcggaccatc atccggaaca
     1621 tcgggtgctc cgtttcgccg tcgttcccct tcccggcgta gaatccaagg aggcatgcgc
     1681 cgccgagcag cgcgccgcac gtgccgttgc tgtgaccgag cccgagggag agccctccca
     1741 tcgaacggat cagcgagtcg ttgcgctctc ctcctgtttg gcgaagcgcc agcagtatga
     1801 gaatctggct gcagtggtac cccttccctt cgagggagag catctccatc tgcaggtcgt
     1861 cgagatcgaa aacatccatt acttgtcctc cagaatcttc tccacttcga gcatcttccc
     1921 cgtggccagc tcttcgggga cgaaaaccgt gccgcaggat gggcagaccg gtaaatccag
     1981 cgtgaagacg ctgccgagat aggtgatctc ggtcttttgc aactccagaa aacggccgca
     2041 tgcgccgcat tcggtcttcg tgtacttctc gaacgacttg atcagctctt tcatgctccg
     2101 tctcctccgg aagcgtcggg gacaagattc tccgcggctc cggcgacgaa cattctgtgg
     2161 ctccacccgt tgcgcacctc gaaagagccg tcgcctttgg gagcgtactc cacccagtag
     2221 gtgaccgacg ccgggcgcag cgaagcgacc agcgcgccgt tgatctgttt catcttgcgt
     2281 ccgcttcgtt ccgccgacca gatggctctc cggacggtat cggcaagaat tcgccgcttc
     2341 gccagagtct tttccacgtc gggcgggatg acaagccgga tggattcatg cggttcgttc
     2401 acgatgtctt cctccttcca gagagatgcg cgcagcgcgc ggaccagtcc gacccggttc
     2461 tcctgctgtt cggagtagcc gatggctgga cgcccggccg ggtcttcgtc tccctgcgga
     2521 aagaggatat ccagcaggtg gacggcgggt ttccccgcct gtgcgaagag attccggcac
     2581 atggcgcagt atacgagaaa gtcaagagaa ctctcctccc cccgggtttt ggcggaaaga
     2641 gcggccaggg tcgggttggc gctctcctgg agtccgccga agccgcagca gaaggagaat
     2701 tctcctgaaa gtaaaggctc ttcgaagcgc actcccgctc gggaggcgac gttccgcacg
     2761 gcgtccctga tttcgggcgc ctgcgcagcg gtacaggggt cggcgacggc aagagttcgt
     2821 tcgaaagaga gcgccccctc cggaagctcc gtgccctcca ggatctccca cagtgagacg
     2881 gccttgattg acggcgttgc cttgcggaag gtctggaggc agctcgtgca ggcggccacg
     2941 acgaccgggg aacccatgga ctcccactct tccgtgatct tcgcggcttc cctgtggagt
     3001 tcttcatcgc gccctgccca gtcggccgga gcgccgcagc agcggagcca gaaaccgacg
     3061 ccgccctcca gacggtccct caaaaaggcg tagagggcgg ttgtctgttc gggagtgcag
     3121 cccgcgagcc ggcatcccgg gaggaagagg taggcgctcg tctcctttcc aggctcgtgc
     3181 cgcaacagcg tcgcccttgg agagttgttg aagcgcatgt cctccagcgc gaagtcgtgc
     3241 gccgaagggg gcatcttctc ctggcggagc atctcccgcc ttgcttcgcg gcagagatcg
     3301 gccatggaga agccgttggg gcagatgcgt tcgcagcggc cgcagagggt gcacgagttg
     3361 atcatgccgt tcgccagcct cgttccctga atcacggaga ggttgttgta gatctcccgc
     3421 gcgaacttct tgggataccc tttgtagtgc tgcatgtagg cgcagtgttt cacgcactcc
     3481 atgcactcgc agaggatgca gcgtttcgcc tccctgacgg cctcttcgag ggagtagcct
     3541 tcctgcggaa ccgcttccgg ctccgctgaa gccatcccct cggtggaggt gaacagacgg
     3601 gtggcgtacg gcccctcctt ctcccgtgag gagaagaggg acgcgccctg gagaaaacgc
     3661 tccgccgact tcattccccg ccgtccgtcg gcggctcgaa ggatgaagga gggggcggcg
     3721 cctccggcga aaagtcctct gagcgaagtc tcaaggctca cggggttcac tgaagcagtc
     3781 gcttccgcag tcgcgcaggc cgcgaacggg tcatccagac cggcgtaggc ggcgtcgaaa
     3841 cgtacggaaa gctctgaaag cggagggagg tcctcaaggg gcagaaacag gactccgcct
     3901 ttttcgagcc aggcaagctc gcggcgcgcg atctcatcgg tgatcagggg ggagagggca
     3961 gtcaacgccg cggcccggtc ctccgcgcag aagacggaga ccgagtaccc tttgcgaatg
     4021 ccttccgagg ctgcgcagag cgcgctcagt ccgcctccga cgacggcgac cctctttccg
     4081 ttcttcggaa taaggagcgg agcgcgccgg agaggcccct gttcgacgca ggctctttca
     4141 agagcgccca actcgatcgc gccgccgagt tcgcggcgga ggcaggcttc cttgcacggc
     4201 gcgtcacaga tacgcgcaag cgcttcgggc agggggagcg tcctctcgat cagcttccgg
     4261 gcgtcgtcga tccggccttc gcggacaaaa gcacagaggg cccgtccgtc gagatgcagc
     4321 ggacaagccg cctggcagta cggaggctcc tcctgaacgc acttcgattc gaattccagc
     4381 agccgtgctt tttccatggc gtacctcccg gaacggaggg aggcgccccg cgaggaacgc
     4441 ctccccggat actgttactt tttcagcccg gccagtacct tctccgggta tgcgggcagc
     4501 ctggtgatac ggacgcccgt cgcgttgtag atgccgttga tgatggatgc gtgcgggctg
     4561 gagagcggaa gctcgccgat gcctgccgcg ccgtgcggcc cgtcttcacg atgttcctcg
     4621 aagtagatga tctccatatc gtcggggatg tccttgatgt acgggaatcc cgcccccggc
     4681 atggtggagt gcttctcgat atcttcgaag tcctcggtga gcgcgaggcc gatgccctgg
     4741 gccatgccgc cccagttctg accgtcgacg acgaggcggt tgttgatctt tccgcagtcg
     4801 cacatcagcg tcatcctgtc gaccgaggtc tttccggttt ccgtgtccac ggtgatctcc
     4861 gccatgaaga cgccgtacat gtagatcacg aacggcttgc cctgtccgtc ctcgttgcag
     4921 gccgttccct cgacggcgga ccacttgccg gtgaaggagg tcggctttcc ggcggcgacc
     4981 agttcgttgt aggtcataaa gccgccgtcc cgtttcagga agcctgtctt cggtttcgcg
     5041 gtctcccgca gcagcgtctc gcaggcgacg cgaatggcgt tccccgtcat gacctgggaa
     5101 cgggagccgc ccgcggggcc ggaattgggg cacttcgccg tattcggcca ggtgaatttc
     5161 agcttgtcgg gggagattcc caggggacgc agcgcctcgt gcgccgtgcc gatcgcgccg
     5221 gcgtcggcgc cctgtccgtg gtcttcccac gcggtgcaga ccgtgatcgt cccgtccggg
     5281 ttcatatcga gccgcgcttc ggagccgtca ggcccgtcga ggccgcagcc gtagacgccg
     5341 accgaaaggc cgacgccctt cttgaagcgg gttgtggagc cctccgcggc gcgcttcttc
     5401 gccagttcgt acttcgggcg gagcgcttcg agcatcttcg gcagcgagta ggactcgggc
     5461 gcctggccgg tcggattggt gcttccgggg cggtaggcgt tcttgtagcg gatctccagc
     5521 gggtccattc ccagcttctc cgccagctcg tccatcagca cctcggagga gaagagcgac
     5581 tgcggcgatc cgtacgcgcg gaaggcggag ccccacgcgt ggttcgtgca gactgtccgt
     5641 ccctcgcccc ggatgttcgg gatgtcgtac ccggcgccca tgaactggat accgcggagc
     5701 gtgaggaggt cgccgaactc ggagtagggg ccgtgatcga ccgagaaatc cgtctccatg
     5761 gcgagcagtt tgccctcttt atcggcggcg aacttcatgt tgacgaagaa gggggagcgt
     5821 ttgccggtgt actgctgctg ctgtttccac gtgtacttca gcgcgaccgg gtgtcccgtg
     5881 gcgagggccg cggcgccgac gagcgcctcc atggtcgggc tgaacttgta gccgaaggtg
     5941 cctccggcgg ggtttgtcac aagaaccagc ttctcgggct cgacgccgag tccgggggcg
     6001 atcatggcga ggtgcaggtg gacgccgatg gactttgagt ggatgacaag gcgtccctcg
     6061 tgatcggtgt acgcgaagcc gacgtcgggc tcgacgggca tgtgcggttg acggccgacg
     6121 tagaaatcgt cttcgaccgt gaccacatcg gagcgtgaga agatggagca gtctcttcgc
     6181 ccttcctgat cttctgcgtg aagtagacgt tcggcgttcc ggggtggatc tcgatggcgt
     6241 cgtcggtcat cgcctcgggc gcgctcatgt acgccggcag gacttcgagg tcgagcttca
     6301 ccttttcggc cgccgcgacg gcgttcttgt agctgtccgc gcagacgatg gcgacggcgt
     6361 cgccgtactg gaagatcttc tcgtcgcaga ggatcgggcg gtcccacccg tctcccttgt
     6421 ttgtcgggaa ggtgatcaga ccggtaatgc ggttctttcc cttgatgtcc ttatgggtga
     6481 cgatcttgta gactccgggc atcttctcgg cttccgaggc gtcgattccc ttgatgttcg
     6541 cgtgggagac ggttgcctgc accagcgccg cgtgcagcgt ctttttgggc atcttaagga
     6601 tgagatccgc gccgtagtcg agcgttccgg tcaccttcgc gacggcggtc gggcgagggt
     6661 acttgctccc ccagatccgt ccgtccgcag gcattttgaa ctcgagctcc ttctcgctca
     6721 tctcgccccg gaggaccttt gcggcgtcca tcacggcatc gacgatgggg atgtacccgg
     6781 tgcagcggca tgcgttccgg ttgagctgga accagtcgcg aacctcttcg cgggtagggt
     6841 tcggattcgt gtcaagcagc cctttcgcgg agacgatgaa gcccggcgtg cagaagccgc
     6901 actgggccgc gccgtgcttt atccacgcct tctggagcgg gtggagcttc tccggcgtgc
     6961 cgatcccctc gatcgtgacg atcttcgtgc cctcggggag gcgggacatc ttgtaaacgc
     7021 acgtgcgggt caccttgccg tccatcagca cgttgcacga cccgcagtgt ccctggccgc
     7081 atcctacctt ggttcccgtg agcccgagct gctcgcggag cacttcggcg agcgttgttt
     7141 cctcgtcgac gatgatgttc cgctcaatgc cgttgacaaa aaagaccttt tgaatcatcc
     7201 tgtttccagc tccttccgaa tctcggcagg ggtcgggacg gcggcgccgc gaaccggggc
     7261 gccggcaagc aacgcggtcc ggaagccgcc gtccttctcc gtatcaaacc acactcttcc
     7321 gccagttttc ctcagtgaag aggagaacgc agtaccgttg cccaggcact cccccttccc
     7381 tcgatgagtc gtgaaacaag aatacaaaaa cgccgtcctc cgtgcaaaga ggacggcgca
     7441 tgccgttttt aggaaatcct ccttgccaaa ggggaggcgc acccgtttcc agatgcaccc
     7501 taacggtcgc agtccgtggg attgttcctg tagggcctgc gactcggagg ttttatctcg
     7561 actgaaacag tcgtcttttt ttgaaaaatg taccttcgat actcattata gagcatcata
     7621 aattcaaggg caacatcagg atggaaattt cgggaaaatg ttttgatttt gcaacatggt
     7681 tgcattttgt gcggcgcttt tcaaaggttt cccacgttct tttgaacaga cggggcggaa
     7741 gaacctcgct gcagcggtac cggaaatacg cggtatacag agttcttcag agttccctcc
     7801 gccgatgaac gcaaattcga cgcgttgtgt ggaggtggtc gtgtcgaggt ggtgtctgta
     7861 tggtagaatg tggatacgaa gtaagtactt tttgttccgt gaagaggagg tgcaccctgt
     7921 ggaagtgaat gttgtaaaag tcggtaattc aaaagggata cggattccgg cccctcttct
     7981 gaagcagctc ggtatagggg agcgggtgat tctggaggtg gagggggatg cgttactcgt
     8041 acgtccggtc agagccccga ggcaagggtg ggcggccgct ttcgggagga tgcgccggag
     8101 cggagacgac gttttgctga tggatgacgc gctcgacgac gatatgctgg aggcgtggga
     8161 tgaaaaacga tagcgagaac ttggttcagt acggcgtgta ctggaccgat ctcgacccga
     8221 cgcagggagc ggagatgaat aagactcgcc cgtgcgttgt cgtctctccg ccggaaatga
     8281 acatgcatct gctgaccgtt ctggttgcgc cggtaacaag cagacaacgt gaaagctatc
     8341 ctacccgcgt tcatttttcg ataggcgacg tttcaggttg gatcgttctc gatcagctca
     8401 gggcgataga caggaagagg ctacgtcgaa agataggatg tctggacgga gagacggctc
     8461 ttctggtgaa gaatgtcatc cgggagatgc tcgtggactg agcagcgtgc cgttgcaggg
     8521 gctctttgcg aagggattcg tccgcccaat actccccggt tttaaaatgc ccggggaagt
     8581 ggccgggcaa ttccttcccg atgaggagcg cgcctacgca aaggacgtag accgagtgaa
     8641 aacccaggta gcgcaggggg acgccgcaac cctccggggt ttttttgctc tggcctccgg
     8701 cggcttcctc cggaagatag ctcgctacgt cagcagcctc agcgccgcga cgaaggcgag
     8761 cgtgaggacg atctggttga agcgctcctg ggagatgcgc ttgactatcc agtatcccag
     8821 cagcgccccc gccgctatgg aggggagggc gagcatgttg atcctgaggc tctgcatggt
     8881 gatgagcccc aggctgaaga agagcggaac cttcagcagg ttcatgatga aaaagaacca
     8941 cgcggtgacg gcgatgaagg gaagtttcgg cagtcctgcg ataagaaggt agaggctcat
     9001 taccgggccg gaagcgttcg ccattcccgt tccgactccg gcgaagaagc cgaaaactgc
     9061 ggtgagcagc ggagatgttt tcccgtcggc cgctgcagcc gccccgcgcc gttgtttcag
     9121 gacgcgcgag acttggtgca ggatgagcat tgcgagcacc acgcctccga tgatgggccg
     9181 caactggtcg tcgctcgcaa agcgcagcac catgtacccc acgccgagcc ccggaagagc
     9241 gaagagcagc agcgtcagaa gacgcctttt gtcggtgtgc atccagaatt ttcccacggc
     9301 gaagaggtcg ccgaaaactg cgatcgcatc aatcgcgctc atgctcgaca
//
LOCUS       DAOTIL010000009         9326 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminobacteriaceae bacterium isolate AMR_MDS_3026
            NODE_8813_length_9326_cov_3.380326, whole genome shotgun sequence.
ACCESSION   DAOTIL010000009 DAOTIL010000000
VERSION     DAOTIL010000009.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868050
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminobacteriaceae bacterium (wastewater metagenome)
  ORGANISM  Aminobacteriaceae bacterium
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae.
REFERENCE   1  (bases 1 to 9326)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9326)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:19:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,224
            CDSs (total)                      :: 3,198
            Genes (coding)                    :: 3,184
            CDSs (with protein)               :: 3,184
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9326
                     /organism="Aminobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8813_length_9326_cov_3.380326"
                     /isolate="AMR_MDS_3026"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:3034609"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..746)
                     /locus_tag="P5201_00360"
     CDS             complement(<1..746)
                     /locus_tag="P5201_00360"
                     /inference="COORDINATES: protein motif:HMM:NF014375.2"
                     /note="Presence of a B(12) (cobalamin)-binding domain
                     implies dependence on cobalamin itself, in one of its
                     several forms, or in some unusual lineages, dependence on
                     a cobalamin-like analog; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0031419 - cobalamin binding [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cobalamin-dependent protein"
                     /protein_id="HRV97014.1"
                     /translation="MADATPFLRGLRGATILGINPPVFDFTFFDLWAKPLGLLFLLDA
                     LRRRGNTVHLLDCIAEGSAEQISFGRRKLLRREISKPAPFRNIPRRYHRFGLDDAEIL
                     RRLHALPRPDCIMVTSGMTYWYEGVFRAVELAKEVFPGVPVLLGGVYARLCPEHAGRS
                     GADIVQDILPDIPFVRPAMELYSEPGYALLLTSFGCPLSCEYCASKLLFPQFIERDLS
                     ETLADAAFQFSLDGVHDAAFYDDALLIDKER"
     regulatory      918..1026
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            1064..1579
                     /gene="thiT"
                     /locus_tag="P5201_00365"
     CDS             1064..1579
                     /gene="thiT"
                     /locus_tag="P5201_00365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012869028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015234 - thiamine transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015888 - thiamine transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupled thiamine transporter ThiT"
                     /protein_id="HRV97015.1"
                     /translation="MFHVRNTILVEGALAVALSVVFSALRLWTMPQGGSITLEMLPIF
                     LFALRRGGKAGCAAGAVSGVMQLITGGYIVHPAQALLDYPLAFGVLGVTGFFKNRPLW
                     LGITLGGVLRFFCHVLSGVVFFGSFAPEGTNVWMYSAVYNGSFMAPTLVVCGVLAYLI
                     WPRLRRVGAEG"
     gene            complement(1554..2636)
                     /locus_tag="P5201_00370"
     CDS             complement(1554..2636)
                     /locus_tag="P5201_00370"
                     /EC_number="3.2.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF012933.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000701 - purine-specific mismatch base
                     pair DNA N-glycosylase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="A/G-specific adenine glycosylase"
                     /protein_id="HRV97016.1"
                     /translation="MVHSSPNITLEEGAAPLLRWYDACRRPFPWRETNDPYAVWISEV
                     MLQQTQAARGVEYHRRWMRIFPTLRSLAEAEDDDVLKAWEGLGYYSRARNLLRAARLL
                     QARGLDTLPASFEELRALPGIGPYTAAAVASIAFGLPVPALDANGKRVFSRLLDLEEP
                     VDRRAGEKSVLRAFVQMLPADRPGDFNSAIMELGATLCAPTSTLCGECPLEFLCLARL
                     RGSALERPVLSRQTKSESLEGILAVFVSGDRVFLRRRPPAGLWANFEEFPWSAPPAEQ
                     ESPLTELARRSNADCDGEVRCSFTKWRLRLAVRIVRLHAPGDLENMNGRWASPSELAA
                     ATLPAGSRKMRERLFGEGTIPPPPPS"
     gene            2829..4856
                     /gene="glgB"
                     /locus_tag="P5201_00375"
     CDS             2829..4856
                     /gene="glgB"
                     /locus_tag="P5201_00375"
                     /EC_number="2.4.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015556796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003844 - 1,4-alpha-glucan branching
                     enzyme activity [Evidence IEA];
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_function: GO:0043169 - cation binding [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0005978 - glycogen biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-alpha-glucan branching protein GlgB"
                     /protein_id="HRV97017.1"
                     /translation="MAKTENIRYDFSLFGEMDSYLFREGNHFRLYEKLGAHVVDDGKL
                     PGTYFALWAPNAREVSVIGAFNDWDRTRHYLSPRKDNSGIWEGFVPGVGKGALYKYSL
                     RSRNGSVFEKGDPFAAFWDIAPKTASIVWPCDYAWNDGEWMKNRRAQNALNAPMSVYE
                     VHVGSWRRKPAEGFRSLSYRELGHELGEYVRAAGFTHVELLPVMEHPFYGSWGYQTLG
                     YFAPTSRYGTPEDFMYMVDVLHQMGIGVILDWVPSHFPADAYGLAHFDGTCLYEHEDP
                     RKGYQPDWGSQIFNFGRNEVREFLISSAFCWLDLFHADGIRVDAVASMLYLDYSRKEG
                     EWIPNQFGGRENLEAVGFLKKMNEAIYQEFPDVQTIAEESTAWPMVTRPTFIGGLGFG
                     MKWNMGWMHDILEYMQKDPVYRKYNHNKITFSIMYAFTENFLLPFSHDEVVHGKGSML
                     GKMPGDEWQQYANLRLLLGYMYAHPGKKLLFMGSEFGQGREWNHDDSLFWDLLHSPRH
                     KGMLDWVTDLNRVYRSEAALHSRDFSPEGFQWCDFSDWEQSVVSFVRKGAGGEMVFAV
                     CNFTPVPRFGYRISVPEGGWWRELLNSDAAEYGGGGVSNLGGAKAEPIPNSSWHSLTI
                     SLPPLGMLLFKLERPEAFPEAVAANRPVSIPVTLPEPAKPPLPEATRRGKK"
     gene            complement(4919..5794)
                     /locus_tag="P5201_00380"
     CDS             complement(4919..5794)
                     /locus_tag="P5201_00380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014163795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HRV97018.1"
                     /translation="MADRPPVSPWFVLSLGVFAISTGAIFARMAEAPPLVIAAYRVGL
                     ATLFLLPFTARRAVREISSLDGSDILRGVASGVFLALHFATWISSLFYTSVASSVVIV
                     NAIPLWTGLLSPFLTGDPFSPSLKKGLLLALPGAVVISWGDFALGWSALWGNFLALQG
                     SFFAALYILLGRKVRPRISLGSYVTLNYGTAAVVLWIVVLAFGLPVAGYSTGTWSAFF
                     LMALVPQLLGHSSYNWALRWLTGGTVALCLLGEPVGSSLLAALFLGEPITLVKVLGGG
                     LILAGIYLASKESGE"
     gene            complement(5802..6827)
                     /locus_tag="P5201_00385"
     CDS             complement(5802..6827)
                     /locus_tag="P5201_00385"
                     /inference="COORDINATES: protein motif:HMM:NF012472.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HRV97019.1"
                     /translation="MKYLPFGKTGVSVSELGFGAIPIIRLTFDEAERVLRAAFDKGIT
                     FYDTANLYHDSEEKIGRALSPVRDKVFLATKSMKRDGKSVAEHIDLSLKRLNTDHVDL
                     FQFHQVAQEKDREAIYAPGGAMEAALAAQQAGKVRFIGVTSHSIPMAMKLIRTGDFIS
                     VQFPFNFIETEAADELHPLARELGVAIIGMKPFAGGAITDASLAFKFLRSHADLFPIP
                     GYDSVESVEQVVSFYDAPNSASEKDFEEMAKVKAELGGEFCRRCEYCQPCPFGVAITG
                     SMIYPLLASRMSPKVASNFAAANLDTVPNCTECGVCVTRCPYTLPIPAMLKKHYELYL
                     EHRNAKE"
     gene            7008..7715
                     /locus_tag="P5201_00390"
     CDS             7008..7715
                     /locus_tag="P5201_00390"
                     /inference="COORDINATES: protein motif:HMM:NF020296.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA alkylation repair protein"
                     /protein_id="HRV97020.1"
                     /translation="MEPLIEELRKELAAHADEETRRSGRRFFKEPVNLYGIKTAVGTK
                     IAKEFFKQLKGRTKEEIFSLCEELWKSGMMEESFVACNWSYALRKEFAEEDFLLFERW
                     IQRYVGNWASCDTLCNHTIGAFIETFPQYVDRLKEWTASENRWVRRASAVSLIIPARN
                     GLFLDDVFEIAERLLLDADDLVRKGYGWLLKVTCAKHEDAVFSYILSRKDVMPRTALR
                     YAIEKMPPERKKEAMKR"
     gene            7862..>9326
                     /locus_tag="P5201_00395"
     CDS             7862..>9326
                     /locus_tag="P5201_00395"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HRV97021.1"
                     /translation="MIPKEPTYSDLQQRIAGLEEALRMKSLAEETMREQETRYRAIVD
                     QATSDALLVHDFQGRFLEVNRRACESLGYSKEELLRMSVTDIECDFDLKSAQAEWSRI
                     EPGIPFILYGHQRRKDGTVFPVEIHFGCSFWNGETFYLGLVRDITKRKLTEEALRESE
                     ERFRNMVEGAPDAIYIQIDRKFAYVNPAACRLFGVASAEELLGMPLLERIHPLYHDVA
                     RERMRRLSDERSPQRERIELRFLRMDGSEVWVETSGEPINYQGKYGALGFARDITWRK
                     EAEEALGESEERFRSLFRDSLSVMFLLDPVTGRFLDVNRECGNFYGWPAQELLNMNIM
                     DIHTPGEEALCEIEKAASGEQNRFESRHRLADGSVRDVEVFTSKICVRGECLLYCIVN
                     DITAKKAAQRQVRLLGRAVEQNSESVIITDAAGNIEYVNPAFTQSTGYAAEDVAGRNP
                     RVLKSGEQSEAFYKRMWETILSGETWRGEMHNKRKNGE"
BASE COUNT         1903 a         2666 c         2982 g         1775 t
ORIGIN      
        1 cgttccttgt cgatcagcag cgcgtcgtcg tagaatgccg cgtcatgcac tccgtcgagg
       61 gagaactgaa aggcggcgtc ggcaagcgtt tcggaaaggt ccctctcgat gaactgcggg
      121 aagagcagct tggaagcgca gtactcgcag gagagcggac agccgaacga cgtcagaagc
      181 agcgcgtacc ccggctcgga gtacagctcc atcgccgggc ggacgaaggg gatgtccggc
      241 aggatgtcct gcacgatgtc cgctcctgaa cgtccggcgt gttcggggca gagccgggca
      301 tacactcccc ccagaagaac gggaacgccg gggaagacct ccttcgccag ctcgacggct
      361 cggaagacgc cctcatacca gtaggtcata ccggacgtga ccatgatgca gtccggacgc
      421 gggagcgcat gcagacgacg gaggatctcc gcgtcgtcca agccgaacct gtggtagcgc
      481 cgggggatgt tccggaaggg agccggtttg gagatttccc tccggaggag cttccggcgt
      541 ccgaaggaga tctgctcggc ggagccttcc gcgatgcagt cgagaagatg gactgtattc
      601 ccccgtctcc tgagagcgtc gaggagaaag aggagcccga gcggtttggc ccacaggtcg
      661 aaaaacgtga agtcgaagac ggggggattg atcccgagaa tggtcgctcc ccgaagcccc
      721 ctgagaaacg gcgttgcatc cgccatgaat gatcagctct tttcagaaga tatggaggaa
      781 gtcttggcgg cttctccttc tttaataata ccatcgcgcc gacggacgca acgaaggcgc
      841 cgcgtcggcg aacctctccc cgtgcagaga gtccggccgt ttttgacagt atttcttggc
      901 aaggtgtata attgcggcgt tcgcatcggg ggagtccgca acggactgag aggggggtgt
      961 tcgtcccccg actcctggaa cctgatccgg gtcatgccgg cgaagggaag gtgccgcttt
     1021 cagggacatt ccaccgatga aaagttgaaa ggaggagtgt tccatgtttc atgtccgcaa
     1081 tactattctc gtcgaaggcg ctctcgcagt cgccctctcc gttgtgttct cggcgcttcg
     1141 tctctggacg atgccccagg gaggctccat caccctcgaa atgcttccga tatttctttt
     1201 cgctctcagg cggggcggaa aagcaggatg tgcggcggga gccgtctcgg gcgttatgca
     1261 gctcatcacc ggcgggtaca tcgtgcaccc cgcacaggct cttctggact atccgctcgc
     1321 gttcggcgtc ctcggcgtaa cgggtttctt caaaaaccgt cctctctggc tggggataac
     1381 gctcggcggc gtgcttcgct tcttctgtca cgttctctcc ggagtcgtct tcttcggctc
     1441 gttcgccccg gaagggacga acgtctggat gtactccgct gtctacaacg gctcgttcat
     1501 ggccccgacg ctcgtcgtct gcggcgtcct cgcctatctt atctggccca ggctcaggag
     1561 ggtgggggcg gagggatagt tccctcgccg aagagccgct cccgcatctt tctgcttccg
     1621 gcgggcagag tcgccgccgc cagttccgac gggcttgccc acctgccgtt catgttttca
     1681 aggtcgccgg gagcatgcag ccgtacaatc cggactgcga ggcgaagacg ccacttcgta
     1741 aaggagcagc gaacttcgcc gtcgcagtcc gcgttgcttc tgcgcgccag ttcggtcaac
     1801 ggcgattcct gctccgcagg gggcgcgctc caggggaact cctcgaagtt cgcccacagt
     1861 cccgccgggg ggcgtcttcg cagaaaaacg cgatctccgg acacgaagac ggcaaggatt
     1921 ccttcgaggc tctccgactt cgtctgccgg gagagtaccg ggcgttcgag ggcgcttccc
     1981 cgaagccgcg cgagacagag gaattcgagc ggacactctc cgcacagggt tgaggtaggc
     2041 gcgcagagcg tcgcgccgag ctccatgatt gcggagttga agtcccccgg acgatccgcc
     2101 ggaagcattt gcacaaaggc cctcaggaca ctcttttccc ctgccctgcg gtcgaccggc
     2161 tcttcaaggt cgagaagccg ggagaagacg cgcttcccgt tggcgtcgag ggctggaacg
     2221 ggaagaccga aggcgatgct cgccacggcc gcggcggtgt aggggccgat gcccggaagg
     2281 gcccgtagct cctcgaagga cgcgggaagc gtatcaagtc cgcgcgcttg cagcagccgc
     2341 gccgcacgaa gcagattcct cgctctggag tagtagccca gcccttccca tgctttgagg
     2401 acgtcatcgt cttccgcctc ggcgagcgag cgcagcgtgg gaaagatgcg catccagcgg
     2461 cgatggtact ccacgcctcg tgcggcctgc gtctgctgga gcatcacctc cgagatccag
     2521 acggcgtacg gatcgttcgt ctcccgccag ggaaagggcc gccggcaggc gtcgtaccac
     2581 cgcagcaacg gcgccgcccc ttcttccaga gtgatgttcg gcgaactgtg taccatgcag
     2641 cgcgctcctt ccttcttcat ctcttcgttt ttcttccata gagcatatgt ataacatgga
     2701 gcgtcatagg gtacaatacg aggggagtat tccggatatt tcaccggctc cgaaaagccc
     2761 tggccataga ggctttcccc ccgttcgttc gagggggcgc gctctatccc gcagaaggag
     2821 gaacggcaat ggcgaagaca gaaaacatac ggtacgattt ttctcttttc ggcgagatgg
     2881 actcatatct cttccgggag ggcaatcact tccgcctgta cgaaaaactg ggagcgcacg
     2941 tcgtcgacga cgggaaactt ccggggacgt atttcgcgct ctgggcgccc aacgcgcggg
     3001 aggtcagcgt gatcggcgcc ttcaacgact gggacaggac gcgtcactat ctctccccga
     3061 ggaaggataa ctccgggata tgggagggct ttgttcccgg agtggggaag ggcgcgctgt
     3121 acaagtattc cctccgctcc cgcaacggga gcgtcttcga gaagggagat cctttcgccg
     3181 ccttctggga tatcgcgccg aagacggcct ccatcgtctg gccctgcgac tatgcctgga
     3241 acgacgggga gtggatgaag aaccgccgcg cgcagaacgc tctcaatgcc ccgatgtcgg
     3301 tctacgaggt gcatgtcggg tcgtggcgca gaaaaccggc cgagggcttc cgttcgctct
     3361 cctaccgcga gctggggcat gagctcggcg agtacgtccg cgcggccggc ttcacgcacg
     3421 tcgagctgct ccccgtcatg gaacacccgt tctacggctc ctgggggtat cagacgctgg
     3481 gctacttcgc ccctaccagc cgttacggaa cgccggagga cttcatgtac atggtggatg
     3541 tgctgcatca gatggggatc ggcgtgatcc tcgactgggt gccgtcgcac tttccggccg
     3601 acgcgtacgg tcttgcacac tttgacggca cctgcctcta cgagcacgag gatccccgga
     3661 aggggtacca gccggactgg ggcagccaga ttttcaattt cggccgcaac gaggtgcggg
     3721 agttccttat cagcagcgcc ttctgctggc tcgacctctt tcacgccgat ggaatacggg
     3781 tggacgccgt cgcttccatg ctctacctcg attactcccg aaaggaaggg gagtggatcc
     3841 cgaaccagtt cggcggcagg gagaatctgg aagccgtcgg ctttctcaaa aagatgaacg
     3901 aggcaatcta ccaggagttc cccgatgttc agacaatcgc cgaggagtcg acggcctggc
     3961 cgatggtgac gcggccgacc ttcatcggag gactcggctt cggcatgaag tggaacatgg
     4021 ggtggatgca cgacattctt gagtacatgc agaaggaccc tgtctaccgt aagtacaacc
     4081 acaacaagat caccttcagc attatgtacg ccttcacgga gaactttctc ctccccttct
     4141 ctcacgacga ggtggtccac ggaaaagggt ccatgctcgg caagatgccg ggcgacgaat
     4201 ggcagcagta cgccaatcta aggctgctgc tggggtatat gtacgcgcat ccggggaaga
     4261 agctcctctt catggggagc gaattcggcc aggggaggga gtggaaccac gacgacagcc
     4321 tcttctggga tctgctgcac tctccccgcc acaaggggat gctggactgg gtgacggatt
     4381 tgaaccgggt ctacaggagc gaagccgccc tccattcgcg tgacttctcg ccggaggggt
     4441 tccagtggtg cgacttctcc gactgggagc agagcgtcgt ctccttcgtc aggaagggcg
     4501 ccgggggaga gatggtcttt gcggtctgca acttcacccc tgtgccgcga ttcgggtacc
     4561 gaatctccgt cccggagggc ggatggtgga gggagctgct caacagcgac gcggcggagt
     4621 acggaggagg cggcgtcagc aacttaggcg gcgcgaaggc ggagccgata ccgaactcct
     4681 cctggcattc gctgactatt tcgctgccgc cgctgggaat gctgctcttc aagctggaac
     4741 gaccggaagc gtttccggag gccgtcgcgg caaatcggcc tgtgtccatc cccgttacgc
     4801 tcccggagcc ggcaaaaccg cctctgccgg aggcgacgag acgagggaaa aagtaacatc
     4861 atacagcgcg gaggagacac gacgttctcc tccgcgtttt ttcttcgtcg aatcgcgtct
     4921 attctccgga ctcctttgac gcgaggtaga tgcccgcaag gatgagccct cctccgagga
     4981 ccttgacgag cgtgattggt tccccgagga agagggcggc gagcagcgaa ctccccacgg
     5041 gctcccccag cagacagagc gcgacggtgc cgcccgtcag ccagcggagc gcccagttgt
     5101 agcttgagtg ccccagcagc tgcggcacga gcgccatgag gaagaacgcg ctccacgttc
     5161 ctgtcgagta gccggcgacg gggaggccga aggcgagcac gacgatccag aggacgacgg
     5221 ccgccgtgcc gtagttcagc gtcacgtagc tccccagcga gatgcgcggc ctgaccttcc
     5281 tgcctaagag aatgtacaga gcggcgaaga agctcccctg gagcgcgagg aagttccccc
     5341 acagcgcgga ccaccccaga gcgaagtcgc cccagctgat gacgacggct cccggaagcg
     5401 ccagcagcag ccccttcttc agcgacgggg agaacgggtc tcccgtcagg aagggcgaga
     5461 gcagccccgt ccacagcgga attgcgttca cgatgaccac gctgctggcc acggacgtat
     5521 aaaagagcga ggagatccac gtggcgaagt ggagcgccag aaagaccccg gaagcgacgc
     5581 cgcgcagaat gtcgcttccg tcgagcgagg agatctcgcg caccgcccgc cgcgccgtga
     5641 atggaagcag aaagagcgtc gccaatccga ccctgtaggc ggcgatgacg agcgggggcg
     5701 cttcggccat ccgtgcgaag atggcgccgg tagaaatcgc gaagaccccc agcgagagga
     5761 cgaaccacgg cgaaacgggc ggtctgtcgg ccatggcgct gttattcctt cgcgtttcgg
     5821 tgttcgaggt agagttcgta gtgtttcttc agcatcgccg gaatcggcag cgtgtagggg
     5881 cagcgggtga cgcagacgcc gcactccgta cagttgggga cggtgtccag gttcgccgcc
     5941 gcgaagttgg aggcaacctt cggcgacatc ctgctcgcca gcagggggta gatcatgctg
     6001 ccggtaatgg cgacgccgaa gggacagggc tggcagtact cgcatcggcg gcagaactct
     6061 ccgcccagct ccgccttcac ctttgccatc tcctcgaagt ccttttcgga ggcgctgttc
     6121 ggcgcgtcgt agaacgagac gacctgctcc accgactcca ccgagtcgta ccccggaatc
     6181 gggaagaggt ccgcgtggga acgaaggaat ttgaaggcca gggaggcgtc cgtgatcgcg
     6241 ccgcccgcga agggcttcat gccgatgatg gccacgccga gttcgcgcgc gagagggtgc
     6301 agttcgtcgg cagcctcggt ctcgatgaag ttgaagggga actggacgga gatgaagtcg
     6361 ccggttcgga tcagtttcat cgccatcggg atgctgtgcg acgtcacgcc gatgaagcgg
     6421 accttcccgg cctgctgcgc cgcgagcgcc gcctccatcg cgccgccggg ggcgtagatc
     6481 gcttcgcggt ccttctcctg ggcgacctga tggaactgga aaaggtcgac atggtccgta
     6541 ttcaggcgct tcaggctcag atcgatatgc tccgcgacgc tcttcccgtc gcgcttcatg
     6601 ctcttggtgg ccaggaaaac cttgtcgcgg accggggaga gcgcccgtcc gatcttctct
     6661 tcgctgtcgt ggtacagatt cgccgtgtcg tagaacgtga tccctttgtc gaacgcggcg
     6721 cggagcacgc gctcggcctc gtcgaacgtg aggcggatga tggggattgc tccgaaaccg
     6781 agttcggaga cggagacgcc ggtcttgccg aaaggaagat acttcatgga tgtcagctcc
     6841 ttcatgcaga ttgtttttcc gactctttct tcgtcaagaa tagcagagaa agagggagtt
     6901 tgaaagagtg ccgacggaaa gctactgggt ggagccttga tgcttttatt tccgcgtggt
     6961 agagttttcc tgaaaaatga tgacgatcct gaaagagagt gatgtctgtg gagccgctga
     7021 tcgaagagct tcgaaaagag ctcgccgccc acgccgacga agagacccgg cggagcggac
     7081 ggcgtttttt caaggaacct gtgaatctct acggaataaa gactgcggtc ggaacgaaaa
     7141 tagccaagga gtttttcaag cagctcaagg ggagaacgaa agaggaaata ttctctctct
     7201 gtgaggagct ttggaaatcg ggtatgatgg aagagtcgtt cgtggcctgc aactggtcgt
     7261 acgcgcttcg taaggaattt gcggaggagg acttcctcct ttttgagcga tggattcagc
     7321 gttacgtggg caactgggcg tcctgcgata ccctctgcaa ccatacgatc ggcgccttca
     7381 tcgaaacatt cccgcagtac gtggaccggc tgaaggagtg gaccgcctcg gagaaccggt
     7441 gggtgcgcag agcgtccgcg gtctcgctga ttatccccgc caggaacggc ctcttcctcg
     7501 acgacgtctt cgagatcgcc gaacggttgc tgctcgacgc cgacgacctc gtgcggaagg
     7561 ggtacggctg gttgctgaag gtgacctgcg cgaagcacga agacgcagtg ttcagctaca
     7621 tcctgagcag aaaggacgtc atgccgagga ctgccctgcg gtatgcgata gagaagatgc
     7681 ctcccgagcg aaagaaggaa gccatgaagc gatagcgtga gagaaggaga gagaaatcct
     7741 gtccttgaaa catgttgtaa aaagatggcg ccgcccgttt ttactgttga acgccgatcc
     7801 tccgcacatc cgacgctccg tgccggaatg ctgcgcggac tgaaagagag atgagggaag
     7861 gatgataccg aaagaaccga cgtacagtga tcttcagcag cgtatcgccg gtcttgaaga
     7921 ggcgttgcgg atgaagagtc ttgccgagga gacgatgcgc gagcaggaga cgcggtatcg
     7981 cgccattgtc gatcaggcga cgagcgacgc cctgctggtc catgatttcc agggccggtt
     8041 cctggaagtc aaccggcgtg cgtgcgaaag ccttggctat tcaaaagaag aacttcttcg
     8101 aatgtcggtg accgatatag agtgcgactt tgatctgaag agcgcgcagg ccgagtggtc
     8161 gagaatcgag ccgggaatcc ccttcattct gtatggacat caacggcgca aagacggtac
     8221 ggtttttccg gttgaaatcc attttggctg ttctttctgg aacggagaga cgttctatct
     8281 tggcctggtg cgcgatatca caaagcggaa actgacagaa gaagccctgc gcgagagcga
     8341 agagcgtttc cggaatatgg ttgaaggcgc gcccgacgcg atttatatcc agatcgacag
     8401 gaagttcgcc tatgtaaatc ccgccgcgtg ccgtcttttc ggcgtcgctt ccgcggagga
     8461 actcctcggc atgccgcttc tggagcgtat tcatcctctc taccatgacg ttgcccggga
     8521 gaggatgcgg cgtctgagcg atgagcgcag cccgcagcgt gaacgtatag agcttcgttt
     8581 tttgcggatg gacgggagcg aagtgtgggt agaaacaagc ggagagccta tcaactatca
     8641 gggaaaatac ggagctctcg gtttcgcccg cgatattaca tggcggaaag aggcggaaga
     8701 agcgctcgga gagagcgaag aacgtttccg cagtcttttc cgggacagcc tttccgtgat
     8761 gtttttgctc gaccctgtta cgggacgctt tcttgacgtc aacagagagt gcgggaactt
     8821 ctacgggtgg ccggcgcagg agcttctgaa catgaacata atggatatcc atacgccggg
     8881 cgaagaagcg ctgtgcgaga tagaaaaagc cgcgtcggga gagcagaacc gtttcgaatc
     8941 caggcatcgt ctggcggacg ggtccgtgcg cgacgtggaa gtgttcacca gcaagatttg
     9001 cgtgcggggc gagtgcctgc tctactgcat cgtcaatgac atcacggcga aaaaagcggc
     9061 gcagcgccag gtacgtctgc tcggcagagc ggttgagcag aattcggaga gcgtcattat
     9121 taccgatgcc gcagggaata tagagtatgt caatccggcg ttcacccagt ccacagggta
     9181 tgccgcggag gatgtcgcag gaaggaatcc tcgcgtgctc aagtccggag agcaaagcga
     9241 ggctttttac aaacgcatgt gggaaacaat cctctccggg gagacgtgga gaggggagat
     9301 gcacaacaaa cgaaagaacg gcgaac
//
LOCUS       DAOTIL010000010         9302 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminobacteriaceae bacterium isolate AMR_MDS_3026
            NODE_8842_length_9302_cov_3.526225, whole genome shotgun sequence.
ACCESSION   DAOTIL010000010 DAOTIL010000000
VERSION     DAOTIL010000010.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868050
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminobacteriaceae bacterium (wastewater metagenome)
  ORGANISM  Aminobacteriaceae bacterium
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae.
REFERENCE   1  (bases 1 to 9302)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9302)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:19:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,224
            CDSs (total)                      :: 3,198
            Genes (coding)                    :: 3,184
            CDSs (with protein)               :: 3,184
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9302
                     /organism="Aminobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8842_length_9302_cov_3.526225"
                     /isolate="AMR_MDS_3026"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:3034609"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..425)
                     /gene="nadE"
                     /locus_tag="P5201_00400"
     CDS             complement(<1..425)
                     /gene="nadE"
                     /locus_tag="P5201_00400"
                     /EC_number="6.3.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006301050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008795 - NAD+ synthase activity [Evidence
                     IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(+) synthase"
                     /protein_id="HRV97022.1"
                     /translation="MSFKCRDAARLASSIEEWLTQKVHAAGAVGGIVGLSGGIDSAVV
                     AALLKRAFGSEMLSVLLPCGSIPKDKEDALLAVKAFDLPYREVDLSPVYDAFVHAFGE
                     PLSPMAAANIKPRLRMNALYALAQTRRFLVCGTGNKAEIL"
     gene            1200..1607
                     /gene="nikR"
                     /locus_tag="P5201_00405"
     CDS             1200..1607
                     /gene="nikR"
                     /locus_tag="P5201_00405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013048266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016151 - nickel cation binding [Evidence
                     IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0010045 - response to nickel cation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nickel-responsive transcriptional regulator
                     NikR"
                     /protein_id="HRV97023.1"
                     /translation="MNSLIRFGVAVPSTLLEEFDSFIERKGMPNRSEALRQLIRERLS
                     REMWISGSGVVYGTVTMMYDHHGKDVVAALTALQHEYGESIICTTHVHVDHHHCLECI
                     VLKGDAGAIRRFVEALATLKGVKSIEPSISAIL"
     gene            complement(1650..1832)
                     /locus_tag="P5201_00410"
     CDS             complement(1650..1832)
                     /locus_tag="P5201_00410"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRV97024.1"
                     /translation="MTEPEKYRIFRCASCGTEFRVEGAGSARCTSCGSKVLILLEGES
                     LSRKKCSGCTASSCST"
     gene            complement(1869..2819)
                     /gene="argF"
                     /locus_tag="P5201_00415"
     CDS             complement(1869..2819)
                     /gene="argF"
                     /locus_tag="P5201_00415"
                     /EC_number="2.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005318430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004585 - ornithine carbamoyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0042450 - arginine biosynthetic process via
                     ornithine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine carbamoyltransferase"
                     /protein_id="HRV97025.1"
                     /translation="MALNLKGRSFLTLMDFTPEEIYYLLDLSADLKAKKRSGIRGTAL
                     QGKNVALIFEKSSTRTRCAFTVACIDEGAHPEFLSKNDIHLGAKEDVKDTARVLGRMF
                     DGIQYRGFKQSLVEELAQYAGVPVWNGLTDVDHPTQILADFLTIQENFGKQLKGIRLV
                     YCGDGRNNMSNALMIGCAKLGMHYVVASPKALFPEKELLAKCSAMASETGGSVTVFED
                     PLVAVKGADAIYTDVWVSMGEEEKKKERQELLTPYQVNMKLLKASGKDSTIFLHCLPA
                     VKGQEVTEDVFEAPNSKVFDEAENRMHTIKAVMVATIGNQ"
     gene            complement(2826..3911)
                     /gene="ispG"
                     /locus_tag="P5201_00420"
     CDS             complement(2826..3911)
                     /gene="ispG"
                     /locus_tag="P5201_00420"
                     /EC_number="1.17.7.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014806645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0046429 - 4-hydroxy-3-methylbut-2-en-1-yl
                     diphosphate synthase activity [Evidence IEA];
                     GO_process: GO:0019288 - isopentenyl diphosphate
                     biosynthetic process, methylerythritol 4-phosphate pathway
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(E)-4-hydroxy-3-methylbut-2-enyl-diphosphate
                     synthase"
                     /protein_id="HRV97026.1"
                     /translation="MSSRQLALRDLTIGGEAPVRVESMLKIPLSLPEDCLAQCFSLRK
                     VGCELVRVAFPSADLRGNLEWLNDRSPIPLMADIHFDGSLALAALESGIPSIRINPGN
                     MGPSHLRSIVASANEKKAVIRIGANGGSLSSSQLDAAGGVRSDALAAAVEEQLRILLA
                     EGFEDIILSAKSTSVMETVRANTLLASKYPDFPMHIGITESGYGRDGLVKSAAGLSLL
                     LAQGIGNTLRISLTEKPEEEVLAGYALLRALDIRSHGVTIVSCPTCGRRRIDVRSILE
                     KLEPSFADLPDALTVAVMGCEVNGPREAMDADFGVAGSPGGAVIFKKGKVLREVSLDE
                     LPGVFADFACSSGENHPSNSCEHLKGE"
     gene            complement(3927..4952)
                     /gene="rseP"
                     /locus_tag="P5201_00425"
     CDS             complement(3927..4952)
                     /gene="rseP"
                     /locus_tag="P5201_00425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014806644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RIP metalloprotease RseP"
                     /protein_id="HRV97027.1"
                     /translation="MVSLVSFLLVIGICVIIHELGHYFTARIFDVQVHEFAFGMGPVL
                     LQRQGRHTMWSFRAFPVGGFVRLAGMGEDHEEEVVEEGRGFFDKAAWKRFLILANGSV
                     SNILLAMLLTVFFLSGHGVINLESTEIGEIMEGYPAQTAGILPGDAIVAVNDVPVSSW
                     REMSESIRTTASEGPVLFSVLRKGETLSLTVTIPPDAQGIPLLGIRPAMRRYSVLEAF
                     ASAFTYTVDMTFEMVRGIIRWITGVEKVDVTGPVGIASMAGDAARRGMWPFVTFLALI
                     NLNLGLLNLFPFPALDGGRLIFTAGEMLLRRRLPARIENFIHMSGFVLLLLLIVYVTW
                     QDILRFF"
     gene            complement(4973..6151)
                     /gene="dxr"
                     /locus_tag="P5201_00430"
     CDS             complement(4973..6151)
                     /gene="dxr"
                     /locus_tag="P5201_00430"
                     /EC_number="1.1.1.267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008711723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0030604 - 1-deoxy-D-xylulose-5-phosphate
                     reductoisomerase activity [Evidence IEA];
                     GO_process: GO:0019288 - isopentenyl diphosphate
                     biosynthetic process, methylerythritol 4-phosphate pathway
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate reductoisomerase"
                     /protein_id="HRV97028.1"
                     /translation="MKKRLFVIGCTGSVGSSVLSVCRMFPEHFEIAALAACSSVDRML
                     LLAEEFSCPTLVFSDESAAVRAANELLSSGVQYAGRRVLAGEQGLLEMASHPDTDHVV
                     FASSGTGAIGALMAALSSGKEVSLANKESIVVAGPWVLPLVLRPDQIRPLDSEHNAIW
                     QCLSGEDRRFVNKIFLTASGGPFRSFSQEEMKRVTPSMAVAHPVWNMGAKISVDSATL
                     MNKGIEILEAMYLFSFPADKVDAVISPNPFIHGLVCFSDGTFHMCASAPDMGIPALSA
                     LFSPLRSPRDRKNTPVLDGMQISFEAPDEVRFPCIRLAREVARKEGAYPALLVGADEV
                     AVDRFLSGRISFMSIPRIVESVLEEYSGPSPSTLEHALHILEEGRRMAEKVSSRLEEK
                     "
     gene            complement(6148..6972)
                     /locus_tag="P5201_00435"
     CDS             complement(6148..6972)
                     /locus_tag="P5201_00435"
                     /EC_number="2.7.7.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013048271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidate cytidylyltransferase"
                     /protein_id="HRV97029.1"
                     /translation="MALSSEKLRELTLRGGSSLVIVAGTLSSLYVGGMVWTFFASAVA
                     LISLAEFYGMLSRKFKVSKGIGFLTSGLIIFSVSEGIHPVSLVLTLSVSAFVVLFVEI
                     IRRQLRGESFAIWNMGGTISGIVYITIPWTFMILLRRLPGGGLLLLAMFIATWSCDVA
                     AYLIGSRWGRTLLCVNVSPNKTWEGFFGGFSAALLASVLLAFILEIPPIPFVYVGIIC
                     GFAGQLGDLAESVIKRESWVKDSGTLIPGHGGVLDRFDSTLINGLLVYVLFGVILR"
     gene            complement(6956..7654)
                     /locus_tag="P5201_00440"
     CDS             complement(6956..7654)
                     /locus_tag="P5201_00440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006301039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016765 - transferase activity,
                     transferring alkyl or aryl (other than methyl) groups
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isoprenyl transferase"
                     /protein_id="HRV97030.1"
                     /translation="MIPKETVPRHVAIIMDGNGRWAKKRFLPRLLGHKAGVKAIERTV
                     RAADALGIQYVSLYAFSTENWKRPEKEVSGLMSIFRFVLRRKVDEMKRENVRLRFSGF
                     RDGMPQDIVELMEWAEEETKENSGLTLVPCLNYGGRQEILHAVENLLEDGVRPPLTEE
                     RFREYLYLPDLPDPDLIVRTSGESRLSNFWLWQSTYSELYFTDTLWPDFDGKELENAV
                     KYFANRERRYGALK"
     gene            complement(7651..8268)
                     /locus_tag="P5201_00445"
     CDS             complement(7651..8268)
                     /locus_tag="P5201_00445"
                     /EC_number="3.2.2.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008520971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004844 - uracil DNA N-glycosylase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HRV97031.1"
                     /translation="MSVFASGADSGLAWEELKRRVETCSSCGLCSTRKKTVFGQGSET
                     SPVVFVGEGPGADEDEQGVPFVGRAGKLLTRILQSVGIERESVFITNVVKCRPPQNRT
                     PMADEMMACTPFLEAQLALLRPKIVVCLGNTPAKWLLKSSEGISALRGRWFSWRGIDL
                     FPMFHPSYLLRNESKKKGSPKDLTWQDINALRSKLDDLTKGGPSA"
     gene            complement(8265..9017)
                     /locus_tag="P5201_00450"
     CDS             complement(8265..9017)
                     /locus_tag="P5201_00450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008711728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRV97032.1"
                     /translation="MVAMENEVDYIIDDGLVIAKGRIMVGKSAKAEKNLVRAIRRALF
                     QFPEHRKAVLAFFQKKQPKKLMIIATSISMAEKITRALRLPYPEMIIDITEVASAEEI
                     INARRERHEKGQHVIPASRTQIRKNFAGKLVGHLRGLFKSVDKEDGERTIVRPPFSFF
                     GELTIESSAILDLVRHVLSSNPQITEVKDIRVRYKDDAISMEIALQVIPGKMDFHRLG
                     VVNQKRVASAVSYFTGMDVDGVDVHIVEVMLP"
BASE COUNT         2349 a         2604 c         2292 g         2057 t
ORIGIN      
        1 agaatctcgg ccttgttgcc cgttccgcat acgaggaaac ggcgggtctg cgccaaggcg
       61 tagagggcat tcatgcggag tctcggtttt atgttcgctg cagccatcgg agaaagaggt
      121 tctccaaagg catgtacgaa agcgtcgtac acaggcgaca ggtcaacctc ccggtacgga
      181 agatcgaatg ctttgacagc gaggagagcg tcctccttgt ctttcgggat acttccgcat
      241 ggaagcagca ccgatagcat ctcggagcca aaggctcgtt tcagaagcgc agccacgacg
      301 gcggagtcta tacctccgct caggccgaca attcctccga cggcccccgc agcgtgaacc
      361 ttctgcgtga gccactcttc aatggagctc gcgaggcgag cggcgtctcg gcatttgaac
      421 gacatcgaca tatccacctt cttctaaaag aaaatccata acctgtttat tttagcatag
      481 tcgttcatat cagagggctg caagtgcata gcgaagctgt ttttttcgaa gggaacgaag
      541 aggatatttt ttccgtgcgc gtgctatgta ttccggccgc aacacgaaga gaagctctcc
      601 ggagtcgctc cgggaagaac cgaggggcgc gcctgttggt ccccagcata caacactctc
      661 tccagaaggc ggaacaagag cggtaaatat ctgattctct actgccctga cgcgagcgat
      721 ttgatcgaaa gggaacgctt cataaacatc aacagaaggg aaacagcata acaactccac
      781 acctgacaaa gcaagcgctc gactatgttc agggatgagc agatccatac ctcctgaaag
      841 accacagaga gagccttgaa cagaaaaagt ggaaaagccg actcgacgac cgggcagctc
      901 tgccggggag ctgattccgc tgtaccgcaa caattcgttt ccaccgtcgt cgcatacaag
      961 cagttcgcct gaaagcattt cattccttgc cgcacaaaaa gctatcctgg acaacgaggt
     1021 gcaagaaagc gtttttgcag aagaaaaagc gaaaaggacg agacatgctg cgctctgaat
     1081 ctccccgtcg ggtgagtgca gcgcgaacgc ggcggaattt ggatacaaat gaagcgcccc
     1141 ccttaaaggg aatttggttt attatactcc atgggggaaa agacaaagga ggagtatttg
     1201 tgaactcatt gatccgtttc ggcgtcgcgg taccatccac actcctggaa gaattcgatt
     1261 cattcatcga aagaaaaggg atgcccaacc gctccgaggc actgcgtcaa ctcattcgtg
     1321 aacgcctttc gcgagagatg tggatcagcg gttccggggt tgtctacggg acagtgacca
     1381 tgatgtacga ccatcacgga aaggatgtcg ttgcggcgct cactgcgctg caacacgagt
     1441 acggcgagag catcatctgt acaacccatg ttcacgtcga ccatcatcac tgcctggagt
     1501 gtattgtttt aaagggagac gccggagcga tacgccgttt tgtggaggcg ctcgccacgc
     1561 tcaaaggcgt taagagtatt gagccgtcga tttcggcaat cctttaatag tgaaaggggc
     1621 agcggaccgc tgccccggtt ttatacgcct caggttgagc aagaggaagc cgtgcatccg
     1681 ctgcactttt tccgggaaag actctcgcct tcgagcagga tgagcacctt gctcccgcac
     1741 gaggtgcatc gtgcgcttcc ggcgccctcg acacgaaact ccgtcccgca tgaggcgcat
     1801 cggaagatcc tgtatttttc gggttctgtc attggcttca cctcccagtc gtactcagag
     1861 acacgtagct actgattccc gattgttgcc accataacag ctttgatagt gtgcattctg
     1921 ttctccgctt cgtcgaagac ttttgaattc ggcgcctcga agacgtcctc ggtcacttcc
     1981 tgccccttca ctgcaggaag gcaatgcaag aagattgttg aatcctttcc ggacgccttc
     2041 aacagtttca tattcacctg atacggcgta agcaactcct gccgttcttt cttcttctct
     2101 tcttcgccca tcgaaaccca gacgtcggta tagattgcat ctgcgccttt tacagctacg
     2161 agcggatctt caaagaccgt tacggagccg cctgtctcgg aagccattgc cgagcacttt
     2221 gcaagcagct ctttttccgg gaagagcgcc ttcggagagg ctacgacata gtgcattccc
     2281 aactttgcac acccgatcat cagggcgttc gacatattat tccgtccgtc cccgcagtac
     2341 acaagacgta tacccttaag ctgtttcccg aaattttcct gtattgtcaa aaagtctgcg
     2401 agaatctgcg tcgggtggtc cacatcggtc aatccgttcc atacgggaac gccggcatac
     2461 tgggcgagct cctctaccag cgactgcttg aatccgcggt actgaatacc gtcgaacatt
     2521 cttccaagca cccgggcggt gtccttgaca tcctctttcg cgcccagatg gatatcgttc
     2581 ttgctcagaa attcaggatg ggctccttcg tcgatacatg cgacagtaaa agcacagcgg
     2641 gttctggtgg acgacttttc gaaaataagt gcaacgtttt tcccctgaag cgccgttccc
     2701 cggattccgg aacgtttttt tgccttcaga tctgcggaga ggtccaaaag atagtaaatt
     2761 tcttcgggag taaaatccat cagagtgaga aaactgcgtc ctttgagatt gagagccata
     2821 gtacatcatt ctcctttcag atgttcgcat gaattactcg ggtggttttc acccgagcta
     2881 caagcaaagt cggcgaaaac accgggaagc tcatccaagg agacttcccg caggactttt
     2941 ccctttttga agatgacggc gccgcctggc gagccggcaa cgccgaagtc ggcgtccatc
     3001 gcctctctcg ggccgttcac ctcgcagccc attaccgcga cggtcagcgc atcggggaga
     3061 tcggcaaaag agggttctaa tttttccagg atggaacgca cgtcaatacg tcttcgtccg
     3121 catgtagggc acgaaacgat agtgactccg tgagatcgaa tgtcaagcgc tctcaaaagg
     3181 gcgtatccgg ccagaacctc ttcctccggt ttctcggtaa gactgatccg gagcgtattg
     3241 ccgattccct gtgcgagaag cagcgagagc ccggcagcgc ttttgacgag tccgtctctt
     3301 ccatagcccg attcggtgat gccgatgtgc atcggaaaat caggatactt cgaagcgagc
     3361 agtgtgttcg cacgcaccgt ctccatgacg gaggtcgatt tcgccgagag gataatatcc
     3421 tcgaaccctt cggcaagcag aatccggagc tgttcttcta cggcggcggc aagagcgtcg
     3481 gatcgaacgc cgcccgcagc atcgagttga gaagaagaga gagagccgcc gttcgcgccg
     3541 atacggatca cggctttttt ctcattggcg gaagccacta tactccgaag gtgagagggc
     3601 cccatgtttc ccggattgat ccggatcgaa ggaattcctg attcgagagc cgcaagagca
     3661 agcgatccat cgaaatggat atcggccata aggggaatgg gcgaccgatc attcagccac
     3721 tccaagttgc cacgaagatc ggccgatgga aatgcaaccc gcaccagctc gcatccgacc
     3781 ttacgcagtg agaagcactg cgcgagacag tcctccggaa gagagagagg aatcttgagc
     3841 atgctctcaa cgcgaaccgg cgcctcgccg cctattgtga gatcacgcag agcaagctga
     3901 cgagagctca tattctttca cttcctctag aaaaaacgaa ggatatcctg ccatgttaca
     3961 tagacgataa gcaggagtaa caaaacaaaa cccgacatat gaataaaatt ttctattcgt
     4021 gccggaaggc gtcttctgag caacatttca cccgctgtaa agatgagacg gcctccgtca
     4081 agggctggga aggggaacag attgagcagc cccaggttca gattgatcaa ggcaagaaat
     4141 gtgacaaaag gccacatccc tctgcgcgca gcgtctcctg ccatggaggc aatacctacg
     4201 ggcccggtga cgtcgacttt ttcaaccccg gttatccagc ggataattcc ccgcaccatt
     4261 tcaaaagtca tatcgacagt gtaggtgaat gcggatgcaa aagcctcaag aacggaatac
     4321 cggcgcatcg ccggtcgtat tccaagaaga ggaatcccct gagcatccgg cggaatggtt
     4381 actgtcaaag agagggtttc tcccttccgc agcaccgaaa aaagaacagg tccttctgag
     4441 gccgttgtac gaatactctc agacatttcc cgccatgaag aaacaggtac gtcgttgaca
     4501 gcgacaatgg cgtcgccggg aagaataccc gccgtttgcg cggggtaccc ctccataatc
     4561 tcgccgattt ctgtgctctc aagattgatc acaccgtggc ccgagagaaa gaaaaccgtc
     4621 agaagcatcg caagaagaat gttggacaca gacccattcg caagaatcag aaaacgcttc
     4681 caggcagcct tgtcgaaaaa ccctcttccc tcctcgacaa cttcctcttc gtggtcctcc
     4741 cccattcctg caagacggac gaaccccccg acgggaaatg ctcgaaaaga ccacatggta
     4801 tggcggcctt gccgctgaag gagcactggc cccatgccga aagcaaactc atgcacctga
     4861 acatcgaata tgcgcgctgt aaaatagtgt ccaagctcgt ggataatgac acaaataccg
     4921 attaccagta ggaacgaaac caaacttacc atttatctta tctccttgac cattattttt
     4981 cttccaatcg tgaactcacc ttttccgcca tccgccttcc ttcttcaaga atatgcagag
     5041 catgctcaag tgtcgagggg gacggtccgg aatactcctc aagcacggat tcaacgatac
     5101 gaggaatact catgaagctg atgcggcctg aaaggaagcg gtcgacagca acctcatccg
     5161 ctcctaccag gagcgccggg taagcgcctt ccttccgggc aacttcacgt gccaatcgaa
     5221 tacatgggaa tctgacctcg tcgggggcct caaaagagat ctgcattcca tccaaaacag
     5281 gggtattctt tctgtcgcgc ggactgcgaa gaggcgaaaa aagagccgat aaggcgggga
     5341 tgcccatgtc cggggcggac gcgcacatat gaaaagtccc gtctgaaaaa caaacaagcc
     5401 cgtgaataaa ggggttggga gagatgaccg catccacctt gtcagcggga aaagaaaaga
     5461 ggtacatcgc ctcaagtatc tcaatcccct tgttcatcag ggtcgcgctg tcaacgctta
     5521 tctttgcgcc catgttccat acggggtgcg cgacagccat tgaaggcgtc acccgcttca
     5581 tctcctcttg agaaaaagag cggaaaggac cgcctgatgc ggtgagaaaa attttgttca
     5641 caaaacgccg atcttccccg gaaaggcact gccaaatagc gttgtgttcg ctgtccaacg
     5701 gacggatctg atcggggcga agaaccagag ggagaaccca cggaccggcg acgacaatgc
     5761 tctccttgtt tgccagggag acctccttgc cgctactgag cgccgccatg agagcgccta
     5821 ttgcccctgt tccggaagag gcaaaaacca cgtggtcggt atcggggtgc gatgccatct
     5881 ccagaagtcc ctgttctccg gcgaggactc ttctcccggc gtattgaacg cccgaagaga
     5941 gcagttcgtt tgcagctcga actgccgcgg attcatcgga aaagacaagc gtcgggcagg
     6001 aaaattcctc tgcaagaaga agcatacgat ccaccgaaga gcacgctgcc agagccgcaa
     6061 tttcgaagtg ctcgggaaac atccggcaaa cggagaggac agagcttccg acgctgcccg
     6121 tacacccgat gacaaaaaga cgtttcttca tcgcaggatc accccgaaaa gtacatagac
     6181 caacagaccg ttgatcagcg tgctgtcaaa tctgtcgagc acacccccgt gacccggaat
     6241 gagggttccg ctgtccttta cccagctttc tctcttgata accgactctg cgagatcgcc
     6301 gagctgtccc gcaaaaccgc agattatccc tacgtagaca aatggaatcg gcggtatctc
     6361 aagtatgaac gccaaaagca cacttgcgag cagcgccgca gaaaagcccc cgaagaaacc
     6421 ttcccacgtt ttgttcgggc tcacgttgac acagaggaga gtgcgccccc agcgcgaccc
     6481 gataagatag gccgcaacat cacagctcca tgtagcgatg aacatcgcca gcaataagag
     6541 ccctcctcca gggaggcgtc gcaacagaat catgaaggtc cacggaatag tgatgtatac
     6601 tatacccgaa attgtacccc ccatattcca aattgcaaaa ctttcccctc gaagctgcct
     6661 gcggattatt tctacaaaaa gcacgacaaa agccgatacg ctcagtgtga gaacgagcga
     6721 caccgggtga atcccctctg atactgaaaa gataataagc cccgaggtaa ggaagcctat
     6781 tcccttggag accttgaact tcctggaaag catcccgtag aattcggcga gagagatcaa
     6841 agcaacagcg gacgcaaaaa atgtccacac cataccgccg acatagaggg aactgagcgt
     6901 acctgccacg atgacgagag aacttccccc tcgcagagta agttcccgta gcttttcact
     6961 tgagagcgcc atagcgtctc tctctattcg caaaatattt cacggcgttt tcaagttcct
     7021 ttccgtcaaa atccggccac aaggtgtccg taaaatacag ctcgctatac gtgctctgcc
     7081 agagccagaa gttactcaac cgactctcgc cgcttgtccg gacaatgaga tcagggtccg
     7141 gaagatccgg aagatagagg tattctctga aacgctcctc ggtcaaagga gggcggacgc
     7201 catcttccag gagattctcc actgcgtgca gaatctcctg gcggccaccg taattgagac
     7261 agggaacgag cgtgagaccg ctattctcct ttgtttcttc ttcggcccac tccatcagtt
     7321 caactatatc ctgcggcatc ccgtcacgaa aaccggagaa gcgaagacgc acattctcac
     7381 gcttcatttc atcaactttt cggcgcaaga caaaacggaa gatactcata agacctgaga
     7441 cctctttttc aggccttttc cagttttctg ttgaaaaagc atacagagaa acatattgaa
     7501 taccaagcgc gtcggctgcc cgtaccgtcc gttcaatagc cttcactcct gctttatgcc
     7561 ccagcagacg cggaagaaag cgtttttttg cccaacgccc gttgccgtcc attattatgg
     7621 cgacgtggcg aggtactgtc tccttcggta tcatgcggac ggccctcctt tcgtcagatc
     7681 atcgagtttg ctgcggagag cgttgatatc ctgccatgtc aaatccttcg gactcccctt
     7741 cttttttgat tcgttgcgga gaagatagct tggatgaaac atcgggaaaa gatcgattcc
     7801 cctccatgaa aaccagcgtc cgcgaagtgc ggaaatgcct tctgaggact tgagcagcca
     7861 ttttgccggg gtgtttccca ggcacaccac gatcttcggc cggagaaggg ccagctgcgc
     7921 ctccagaaat ggcgtgcatg ccatcatttc gtccgccatc ggagtgcggt tctgcggggg
     7981 ccggcacttt acaacattcg taataaaaac gctttccctc tcaataccga ccgattgaag
     8041 aatacgcgtg agcagttttc ccgctcgtcc tacaaatggg actccttgtt catcctcgtc
     8101 cgctccggga ccttcgccga cgaaaacaac cggggaggtc tcgcttccct gaccaaaaac
     8161 cgtcttcttt cgcgtcgaac acaggccgca cgatgaacag gtttctacac gccttttcag
     8221 ttcctcccat gccaacccgc tgtcggctcc gctcgcaaag acgctcatgg cagcattacc
     8281 tccactatgt ggacgtcgac accgtccaca tccattcccg taaagtagct gactgcggat
     8341 gctactctct tttgattgac tacaccaagt cggtggaaat ccattttccc cggaataacc
     8401 tgaagtgcaa tctccataga aatagcatca tctttatatc gaacacgaat atccttcact
     8461 tctgtaatct gcggattcga agagagcaca tggcggacaa gatcaaggat agccgacgac
     8521 tctatagtca attctccaaa aaaactgaat ggagggcgaa caattgttcg ttctccgtcc
     8581 tccttgtcga cgcttttaaa gagtccccgc agatgtccaa ctagttttcc ggcgaaattt
     8641 ttccgtatct gcgtcctgga ggcgggaata acgtgctgtc ctttttcatg acgctctctt
     8701 ctggcattga tgatttcttc tgccgaagct acctccgtta tatcgatgat catttccgga
     8761 tacggaagac gaagcgctct ggtgatcttc tccgccatcg aaatcgacgt tgcaataatc
     8821 atcagcttct tcggttgttt cttctggaaa aaggccaata ccgctttcct atgctccggg
     8881 aactgaaaaa gagctcttcg tattgcacgc acaaggttct tctcagcctt tgcactcttt
     8941 cccaccatta tacgtccctt ggcaataaca agcccgtcgt caattatata gtcaacctcg
     9001 ttttccatgg cgaccatctg cgcccgttga ctcttaccgg tacctgcagg accaacaaaa
     9061 gcaataactc ttgtcacacc ggaatcgttt aacgccatga cgacatacca tagagcagga
     9121 aaagagacat tccggccggc tttaagaacg aagcgccgga gaatcccctt tggaatcctc
     9181 tacggatggg agaatcgaaa tatttgctcc gatcgcgcgc aacttttctt ccataagttc
     9241 atacccgcgt gaaatatgcc cgagatgatg aacataggta atatctcttg ctgcaagccc
     9301 cg
//
LOCUS       DAOTIL010000011         9039 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminobacteriaceae bacterium isolate AMR_MDS_3026
            NODE_9187_length_9039_cov_3.724622, whole genome shotgun sequence.
ACCESSION   DAOTIL010000011 DAOTIL010000000
VERSION     DAOTIL010000011.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868050
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminobacteriaceae bacterium (wastewater metagenome)
  ORGANISM  Aminobacteriaceae bacterium
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae.
REFERENCE   1  (bases 1 to 9039)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9039)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:19:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,224
            CDSs (total)                      :: 3,198
            Genes (coding)                    :: 3,184
            CDSs (with protein)               :: 3,184
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9039
                     /organism="Aminobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9187_length_9039_cov_3.724622"
                     /isolate="AMR_MDS_3026"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:3034609"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(196..906)
                     /gene="pyrH"
                     /locus_tag="P5201_00455"
     CDS             complement(196..906)
                     /gene="pyrH"
                     /locus_tag="P5201_00455"
                     /EC_number="2.7.4.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014807253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033862 - UMP kinase activity [Evidence
                     IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UMP kinase"
                     /protein_id="HRV97033.1"
                     /translation="MKFNRVLLKLSGEVLAGNNGFGFDFDAVRRIGEEIVEVARSGIQ
                     IAMVVGGGNMLRGKELESLGVERSQADYMGMLATVMNALCLQDVLEKFGVPTRVQTAI
                     EMRQMAEPYIRRRALRHLEKERVVIFAAGTGSPYFSTDTAAALRAAEVGADCLLKATK
                     VDGIYSADPKKFPDAVLLHRLTYMEALQRQFEVMDAAAFSLCMENAIPIVVLNILRKG
                     NLKDFLVDGKNTGTMVSA"
     gene            complement(982..1575)
                     /gene="tsf"
                     /locus_tag="P5201_00460"
     CDS             complement(982..1575)
                     /gene="tsf"
                     /locus_tag="P5201_00460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008520931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation elongation factor Ts"
                     /protein_id="HRV97034.1"
                     /translation="MEISASAVKELRERTGAGMMDCKNALAENGGNVEKAIDYLREKG
                     LAKAAKKAARTAADGRVFSYIHTNGKLGALIELNCETDFVAKTDEFQNLGHELCMHIA
                     AAAPQFLSSEEVTADVLEREREIYKQQALEEGKPANIVDKIADGKINKFYELNCLLEQ
                     NWIRDGDKKIKDLIVEVIAKLGENIVLRRFSRFSIGE"
     gene            complement(1745..2548)
                     /gene="rpsB"
                     /locus_tag="P5201_00465"
     CDS             complement(1745..2548)
                     /gene="rpsB"
                     /locus_tag="P5201_00465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013048575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S2"
                     /protein_id="HRV97035.1"
                     /translation="MAVVSMKQLLECGVHFGHQTRRWNPKMKPFIFTERNGVYIIDLQ
                     KTVRGLEKAYDYIREVARNNGTVLFVGTKRQAQDTIKDEAERCGQFFINQRWLGGMLT
                     NFPTIRKRVARMVELRKMEDEGNWSNLPKKEVIILRKELGKLEKYLKGITNMKAIPDA
                     IFLIDPRREENAVLEARKLGVPVIAIVDTNCDPEMIDFPIPGNDDAIRAIKLIAGLMA
                     NAFIEGRQGEDSEAGVLVQGEEGDDTADDGIIEKKERLHEVYDDSVEEA"
     gene            2762..3100
                     /locus_tag="P5201_00470"
     CDS             2762..3100
                     /locus_tag="P5201_00470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013048576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-sensitive transcriptional regulator"
                     /protein_id="HRV97036.1"
                     /translation="MAKDSLIEKLEKLPKDRKAMLNRLRRVEGQLRGIQRMIIEEKPC
                     YDILLQLSAARKAMQKACIEILKNYLHRCVHEAKAPDFDNLEKLIEALIEISPAKPAF
                     PENEEESDEE"
     gene            complement(3120..3219)
                     /gene="ffs"
                     /locus_tag="P5201_00475"
     ncRNA           complement(3120..3219)
                     /ncRNA_class="SRP_RNA"
                     /gene="ffs"
                     /locus_tag="P5201_00475"
                     /product="signal recognition particle sRNA small type"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00169"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00169"
     gene            complement(3296..4090)
                     /locus_tag="P5201_00480"
     CDS             complement(3296..4090)
                     /locus_tag="P5201_00480"
                     /inference="COORDINATES: protein motif:HMM:NF012707.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HRV97037.1"
                     /translation="MYGKKRVSSVFCVLLVLFLFSIAGQAAAGARLDRVMETKLLRVG
                     TPGDYRPFAMLDKSTGKYEGHDIELAELLASELGVTVEFVPTTWSKLMEDYLAGKFDV
                     AVGGITRSLARMLKGDFLPPYAPNGKVAIIRKADKEKFTSLEAMDVPETTVIVNPGGT
                     NEKYVTANFKSAKVVVHQSNAEIPAMIAEGKGDVMISEVYEAVVYSRKDDRLYGAFTD
                     KPLTKISFMGFFIQQDDPDFLKIMHYLWNDAKLRGDLDALFAKWLK"
     gene            complement(4148..5206)
                     /locus_tag="P5201_00485"
     CDS             complement(4148..5206)
                     /locus_tag="P5201_00485"
                     /inference="COORDINATES: protein motif:HMM:NF024799.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter substrate-binding protein"
                     /protein_id="HRV97038.1"
                     /translation="MNSSFRKVIPALLALFVFSAASCASEKQISIGMIIKEPSAPYIQ
                     AFIKAAEETAASLGVNIMIRDGEADSIKIMEIIDTFMIQRIDAFILGGAVDLRTLVPG
                     IVRLNEAGIPVAALDTSPEGGVVDFFLSFDLAQSSARAAELFVEGIKSRNNGDIPEGV
                     VLEILGDKADMFSHACTEGFNSVLSKYPQLEIAQGEGKWNNTDSHAVTSDLLTRFGKR
                     VVGIYVQTPDIMGPGVVAAIEAAGLDSADYGICGICIGPEGIDLIKKGKMLGAVAQPA
                     YDSAALAVRFLVDKLRGNPVPRIGDTLTEDGALWSPAKVIKNPWAEDGAFIVLQGPLV
                     PSEVSPDDPRLWENMLTK"
     gene            complement(5274..6290)
                     /locus_tag="P5201_00490"
     CDS             complement(5274..6290)
                     /locus_tag="P5201_00490"
                     /inference="COORDINATES: protein motif:HMM:NF014684.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HRV97039.1"
                     /translation="MSGSLKIKLTLLDNIIWLMLFLFFFACALGIPAFASWNNIVNIL
                     YHTTIMSMLVLAQGFVLLNGNFDLSIDAVLAFAPGVVILASTKWFPVALGNPYLAILL
                     TLLLGGCIGFFNGYCVAKLGMNAFMQTLSLSIILRGMVLFLIPLSIFPLDPVYSFIGK
                     ARFSAVGNLPAAIPVTFAIYFLFHILLRHTVFGRNYLATGGNARASFVAGIDTQKMVI
                     AGFVIAGLLSAVAGMLTAGRQDSVSNTMGNGMTLLAFAGALLGGTSMSGGKGSAFGML
                     GGAILLGMFSNALNLLGVKVTLIHAAQGALIFVAILTDRFRISLRARLLRNEQLQKLK
                     VAKG"
     gene            complement(6275..7822)
                     /locus_tag="P5201_00495"
     CDS             complement(6275..7822)
                     /locus_tag="P5201_00495"
                     /EC_number="7.5.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter ATP-binding protein"
                     /protein_id="HRV97040.1"
                     /translation="MEEVCSKPVLLLEGISKVYPGTVALENIHFEALPGEVHGLIGKN
                     GAGKSTLVGILSGLVEPTSGTIMVNGRSFSSFSRSRAKREGISIVPQEPEIVLDLSVA
                     ENLFLGELENCSGFVSWSKIRADAQRILDRFGLDIGADLQARDLSLSERQILLILKAC
                     VVEDANVVVLDEASASLSRNDAAILKRIVGELRESGKAVLYISHHIDELLDICDRLTV
                     LRDGRIVTTRTKNMLDHHSLAELIVGETLSSSGPERKEAGYIGEELLRLENLTRWGNF
                     CNISFSLKKGEILGIAGLRGSGRTELLKAISGIEPADAGKIFFKDKRVSFSTPSEALR
                     MGVAYLPEDRETEGLIQTFSIRENLLLNSISRFSRNGFVERRAAGRRAEEIFAGVQIH
                     AFSMEQSAEELSGGNKQKVVIGRIMANAPEVYLLDEPTRGVDIGAKRSILSIIHGPLR
                     AASGVLLTSPGLDDLMDICDRILVLYKGNMVREYQQKDFDEKSLFMDVQGFGYQDQRG
                     GDECRAV"
     gene            complement(7822..>9039)
                     /gene="hisD"
                     /locus_tag="P5201_00500"
     CDS             complement(7822..>9039)
                     /gene="hisD"
                     /locus_tag="P5201_00500"
                     /EC_number="1.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005661055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004399 - histidinol dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol dehydrogenase"
                     /protein_id="HRV97041.1"
                     /translation="ECSVESVVGSILKSVRDSGDEAVLAYTEKFDGVRPSSLRIGKEE
                     MRSAYEKVAPSTVKTLEFAAERIRNFALRQKSCMQELRYEISPGVTLGHRLLPMESCG
                     CYVPAGRYPLPSSALMSAIPAKVAGIRRIAACSPAGKGRDGIHPAVLVAMDIAGVDEV
                     YCMGGAQAIGAFAYGTETIKGVDIIVGPGNRYVTEAKRQVSGAVGIDMLAGPSEVVIL
                     ADESANPEWVAADALARCEHDPHSWTVLVTTSRRLGESVMSAVETQAPLLSTGEFAFQ
                     SWRNNGRVLLAESLDEAVAITNDIAPEHLQVMTSDSPAISSQLVNFGSLFIGENAPVV
                     FGDYASGPNHILPTVRCARFSNGVYVGTFLRVCSFQELTAEGAAYLAGPCADFAEMEG
                     LFGHKRSAEMRAR"
BASE COUNT         2147 a         2543 c         2213 g         2136 t
ORIGIN      
        1 ctctcagttc tttcagctca gttttcggca ttccggaatt cctccttcga tgaagaatag
       61 atccccgaaa aatgggggcg gaacagtcaa gcatccagtg cagaactaaa aaacactccg
      121 gcgtctcctt cggcatgcga tcagccgacg atacgctgga tttctgcgcg cgcacgtctc
      181 tctttcgaga ctctcctacg ccgataccat ggttcccgtg ttcttcccgt caaccagaaa
      241 atctttcagg ttgcccttac gcagtatgtt caatacaacg ataggtatgg cattctccat
      301 acagagcgag aaagcggccg catccattac ctcgaactga cgctgaagag cttccatata
      361 ggttaagcgg tgcagcagaa ccgcatccgg aaattttttc gggtccgcac tatatatacc
      421 atctactttc gttgctttca acaggcaatc tgctcccacc tccgccgcac gaagcgcggc
      481 ggccgtgtcc gtagagaaat aaggagagcc tgttcctgcc gcaaagatga cgacgcgctc
      541 tttttcgaga tgacgcaagg ccctgcggcg aatgtacggt tcagccattt ggcgcatctc
      601 gatcgctgtc tgtacacgtg tcggcacacc gaacttttcg agaacatcct gaaggcagag
      661 ggcgttcata accgtggcca gcattcccat atagtcggct tgggaacgtt ctaccccgag
      721 ggactcaagt tcttttcccc gaagcatatt gccccctcct acgaccatgg caatctggat
      781 acccgagcgg gcgacctcaa cgatctcctc gcctatccgc cggactgcgt caaaatcgaa
      841 gccaaatccg ttatttcccg caagaacctc tccggagagc tttaaaagaa ctctgttgaa
      901 tttcatcgga attcctcccc ttctcaagat ttcgggtatg aaaaaggcga ggctttcggc
      961 cccgcctttt tttataaaaa actattcacc gatcgagaat cgggagaagc gtctgaggac
     1021 aatgttttcg ccgagttttg cgataacctc tacgatgaga tccttgatct tcttgtcgcc
     1081 gtctcggatc caattctgct cgagcaggca gttgagctcg tagaacttgt tgatcttacc
     1141 gtcggcgatt ttatcgacga tatttgcggg tttcccttct tcaagggcct gctgcttgta
     1201 gatctcacgt tctctttcca acacgtcggc ggttacttcc tccgaggaaa ggaactgcgg
     1261 cgccgcagca gcgatgtgca tgcacagttc atgtccaagg ttctggaatt catctgtctt
     1321 tgcaacaaaa tcagtttcac agttcagctc gatcaaagcg ccgagtttgc cgttcgtgtg
     1381 gatatacgag aaaacacgac cgtctgcggc agttctggcg gccttctttg cagctttggc
     1441 aagacctttt tcgcgaaggt agtcgatagc cttctccacg ttcccgccgt tttcggcgag
     1501 ggcgttcttg cagtccatca tccccgcgcc tgttctttca cgaagttcct ttacagcgct
     1561 ggcgctaatt tccataataa aaaagcctcc ctttccggta aacaaacttc cccgctcaac
     1621 gaggaaaaaa tccaattcag aaacacattg aggaacgtat tcttctgaca gatggctgaa
     1681 aaaccggcac acgaagaacc gacccaacag gatttcccgg tcgggtcggc ttcgtctcta
     1741 taacttacgc ctcttcgaca gaatcgtcgt agacttcgtg caagcgttcc tttttctcta
     1801 taataccgtc gtcggctgta tcgtcgccct cttcgccctg aacaagcacg ccggcttcgg
     1861 aatcctctcc ctggcgtcct tcaatgaaag cgttggccat aagtccggca ataagcttga
     1921 tcgcgcggat ggcgtcgtcg ttgccgggga tcgggaagtc gatcatctcc ggatcgcagt
     1981 tcgtgtcaac tattgcaatc acgggcactc ccagtttcct cgcttcgagg acggcatttt
     2041 cctctcttct agggtcgata aggaagatgg catccgggat ggctttcatg tttgtgatgc
     2101 ccttaagata cttctccagt tttcccaact ctttgcgaag aataatgact tctttcttgg
     2161 ggagattgga ccagttgccc tcgtcttcca ttttgcgaag ttccaccata cgggcaacgc
     2221 gcttgcgaat cgtcggaaag ttggtgagca tgccgccgag ccagcgctgg ttgataaaga
     2281 actgcccgca acgttcagcc tcgtccttga tggtatcctg agcctggcgc tttgttccga
     2341 caaaaagaac cgtcccgttg ttcctggcaa cctcgcggat gtaatcgtat gctttttcaa
     2401 ggccgcggac agtcttctga aggtcaatga tatacactcc gttgcgctcc gtgaagatga
     2461 aaggcttcat cttcggattc caccgacgag tctgatgtcc gaaatggact ccgcactcca
     2521 aaagctgttt catgctgaca actgccaaaa taacccctcc tgaaatatgt tggttttccc
     2581 tccacaaaca tcgaacaaag tggaaaccgt tttcttgaaa cggcaccctc caccccatca
     2641 gtttgtgtgt gtgatgagga ccaggagagt ataccacaaa gacaggttga ccttcaacct
     2701 gtctcgaagt accatatacc cagtatttac tctactgaaa aaaccgaatg ggggcatagt
     2761 aatggccaag gattcactta tcgaaaaact cgaaaaacta cccaaggaca ggaaagcgat
     2821 gttgaaccgt cttcgccgcg tagagggcca gcttcgcggc attcagagga tgatcattga
     2881 agaaaaacct tgttatgata tcctccttca actctcggca gcgcgaaagg caatgcagaa
     2941 agcatgcatt gaaattctaa aaaactacct ccacagatgc gtccacgaag cgaaagcgcc
     3001 tgatttcgac aatctcgaga aactcattga ggctctcatt gaaatatctc cggcgaagcc
     3061 tgcgttcccg gagaacgagg aagagtctga cgaagagtaa tacagaacgg ctgcctgcgg
     3121 gaaacccagt ttccccgtgg cgcccggaga attctcctta ctgctgctcc cttccgggcc
     3181 tgacagggtt cgaaggtctc cgccgcacgg gactggattc cccgcaggca gccgttcatt
     3241 ctgcacgttt tcagcgcagt ggattatagc atggcttcga gcactttacc agcgactact
     3301 ttaaccactt tgcgaaaaga gcgtccagat ctccccgcag cttcgcgtcg ttccagagat
     3361 agtgcatgat cttcaggaag tcggggtcgt cctgctgaat gaaaaatccc atgaagctga
     3421 tcttcgtcag cggtttgtcg gtaaaggcgc cgtagagccg gtcatccttc cgcgagtaca
     3481 cgacagcttc gtacacttcg ctgatcatca cgtcgccttt cccctctgcg atcattgcgg
     3541 ggatctcggc attgctctga tgcactacga ctttcgcact cttaaagttt gcggttacat
     3601 atttttcatt tgttccgcct gggttcacaa tgactgtcgt ctcgggaacg tccatcgctt
     3661 caagcgaagt gaacttctcc ttgtcagcct tccgaatgat ggcaaccttg ccgtttggtg
     3721 catacggagg caaaaagtcc cccttcaaca tacgggcaag gctccgggtt atacctccga
     3781 cggcgacgtc gaatttgcct gcaaggtaat cctccataag cttcgaccag gtggtgggaa
     3841 caaattcaac ggtcacgcca agctccgacg caagaagttc cgcaagttcg atatcgtgcc
     3901 cctcgtattt gcccgtactc ttgtcgagca tggcgaatgg gcgataatcc ccgggagtcc
     3961 ccacccgcaa tagctttgtt tccataacgc ggtcaagcct cgctcctgct gcagcctgtc
     4021 ccgcaatcga gaaaagaaac agcaccagca gtacacaaaa tacactcgaa actctctttt
     4081 ttccgtacat caatacccct ccgttcgttt tttgtttcgt aacattctct ctcatctcaa
     4141 aacccgttta cttcgtcagc atattctccc acagacgggg gtcgtcaggg gagacttctg
     4201 aaggaacaag cggtccttgg agaacgataa aggctccgtc ttcggcccaa gggttcttta
     4261 tgacttttgc gggagaccag agagcgccgt cttccgtcag cgtatctcct atccgtggaa
     4321 caggattccc tctcagcttg tccacaagga acctcaccgc cagcgcggcc gagtcgtagg
     4381 caggctgtgc gacagcgccg agcatcttcc cttttttaat gaggtcgatt ccctcggggc
     4441 cgatgcatat tccgcagata ccgtagtcgg cggaatccaa ccctgcggcc tcgatagccg
     4501 caacgacacc gggtcccatt atatcagggg tttggacata aatgcccaca acccgtttcc
     4561 cgaaacgcgt caacaaatcg gaagtcactg cgtgggagtc ggtattattc cattttccct
     4621 ctccttgagc aatttcaagc tgcggatact tcgacagcac ggagttgaat ccctcggtgc
     4681 aggcatggct gaacatatcc gctttgtcgc cgagtatctc aagaacgact ccctcgggta
     4741 tatcgccgtt gtttctggac ttgatcccct cgacgaaaag ctccgcggcg cgggcgctcg
     4801 actgagcgag gtcaaaggag aggaagaaat cgacaacgcc tccttcagga gatgtgtcca
     4861 gtgcggccac ggggatccca gcctcgttga ggcgcacaat accaggcacc agcgtgcgca
     4921 aatccacagc gccgccaagg atgaacgcgt cgattctctg tatcataaaa gtatcgatga
     4981 tttccataat ttttatagaa tcggcttcgc cgtcgcgaat cattatattc acgcccagcg
     5041 aggcggctgt ctcttccgct gcctttataa atgcctgaat gtacggcgcc gagggttctt
     5101 tgatgatcat accgatggag atttgctttt cggacgcaca tgaagccgca gaaaaaacga
     5161 aaagcgccag aagcgccggt attacttttc taaaagagct gttcacgtag attcctcctc
     5221 gatgtagtgg atgaacgtac tttaaaatct ttcctcattc tcaattattg ccatcatcct
     5281 ttcgcgactt tgagtttttg gagctgttcg ttacgaagca atcgtgcccg gagggagatt
     5341 cgaaaccggt ctgtaagtat ggcgacaaat atcaacgccc cctgagcggc gtgtatgagc
     5401 gtcaccttga caccgagaag attaagagca ttcgaaaaca tgcccaaaag gatcgcaccg
     5461 ccgagcatcc cgaaagcgga gcctttccct ccgctcatgg aggtgcctcc gagaagcgct
     5521 ccggcgaatg caaggagcgt cattccgttc cccatggtgt tggacaccga atcttgcctc
     5581 ccggctgtca gcataccggc aacagcggag agaagcccgg ctatgacaaa acctgctatc
     5641 accatttttt gtgtatctat acctgcgacg aagctggccc gtgcatttcc gcccgttgcc
     5701 agatagtttc tgccgaagac ggtatggcgt agcagaatgt gaaagaggaa gtagattgca
     5761 aatgtcacag gtatagccgc agggaggttt ccaacggctg aaaaacgcgc cttgccgata
     5821 aaggaataga ccggatccaa cggaaatatc gaaaggggaa taagaaaaag gaccattccc
     5881 cgaagaataa ttgaaagaga aagcgtctgc ataaaggcgt tcattcccag cttggcaaca
     5941 cagtagccgt tgaaaaaacc gatgcacccg cccaggagca gcgtgaggag aatcgccagg
     6001 tacggattac ccaacgcgac gggaaaccac ttcgtcgagg ccaaaataac tactccgggc
     6061 gcaaaggcga gcacagcgtc gatagaaagg tcaaaattgc cgtttaagag aacgaaacct
     6121 tgtgcaagca caagcatgct cattatcgtg gtatggtaca gtatgttcac aatgttgttc
     6181 catgaggcga atgccggaat tcccagggcg cacgcgaaaa aaaagagaaa gagcatcagc
     6241 cagatgatat tgtccaaaag agttaacttg atttttaaac tgcccgacat tcgtcgccac
     6301 ctctttgatc ttgatatcca aaaccttgca catccataaa aagagatttt tcatcgaaat
     6361 ctttttgttg atattcgcga accatgttcc ccttgtacag aacaaggatt ctgtcgcaga
     6421 tatccatcag atcgtccagt cccggcgacg tgagcagtac gccggaggcg gcgcgcaacg
     6481 gtccgtgaat tatggagagg atggaacgct tcgctccaat atccactcct cgcgtcggtt
     6541 cgtcgaggag atagacctcc ggtgcgttcg ccataatacg tccaattacg accttctgct
     6601 tatttcctcc ggaaagttcc tcggcgctct gttccatgga aaacgcgtga atctgtaccc
     6661 cggcgaagat ctcttccgcg cggcggcccg ccgctcttct ctcaacgaag ccattccggg
     6721 agaatctgga aatgctgttg agcagcaaat tttcgcggat gctgaacgtt tgtatcagcc
     6781 cctccgtctc gcgatcctcc ggaagatacg ctactcccat gcgaagggct tccgaggggg
     6841 tggagaaaga tactcttttg tctttgaaga aaatttttcc tgcatcggca ggctctatcc
     6901 ccgagatcgc cttcagcagc tcggtccgtc cgctcccccg caatccggcg ataccgagaa
     6961 tttctccttt tttgagggaa aagctgatat tacaaaaatt cccccatcgt gtaagatttt
     7021 cgagacgaag gagctcttcg ccgatatagc ctgcttcctt tcgttcgggg cccgaagaag
     7081 aaagcgtctc tccaactatc agttcagcga gcgaatgatg gtcaagcata ttcttcgtcc
     7141 gtgttgtgac gatacgtccg tcccgaagta cggtaagtcg atcgcaaata tcgagcagtt
     7201 cgtcgatatg atgggagatg tagaggactg ccttccctga ctccctcaat tcacccacaa
     7261 ttcttttcag aattgcggcg tcgttccggc tgagcgacgc cgatgcttcg tcgagcacaa
     7321 caacgtttgc gtcctcgacg acgcatgctt tcagaataag aagtatctgc cgctcgctga
     7381 gagagagatc tctcgcctga agatcagcgc cgatatcgag tccgaaacga tcaagaatgc
     7441 gctgggcatc cgcccggatc ttgctccatg aaacaaaacc ggaacagttt tccagctctc
     7501 ccaggaagag attctctgca accgaaagat caagcactat ctccggttcc tgtggaacaa
     7561 tggagatccc ctcacgtttt gctcgcgatc tgctgaacga tgaaaaggat cgtccattta
     7621 ccatgatggt tccggaggtc ggctccacaa ggcccgagag aataccgacg agcgttgact
     7681 tcccggctcc gttcttcccg atgagaccgt gcacttctcc cggaagcgct tcgaaatgaa
     7741 tattctcaag agccactgtt cccggataga ccttgctgat cccctcaagc agcaaaactg
     7801 gtttggagca gacttcttcc atcaccgtgc acgcatctcc gctgaacgct tgtgtccgaa
     7861 aaggccttcc atttcggcga agtcggcgca tggtcccgca aggtaagcgg ctccttccgc
     7921 agtgagctcc tggaaggaac agactctcag gaaagtcccg acgtagacgc cgttcgaaaa
     7981 gcgggcgcac ctgacggtag gcaaaatatg attcggtccc gaggcatagt cgccgaaaac
     8041 gaccggtgcg ttctccccga tgaagaggga accgaagttt accagttgtg acgaaattgc
     8101 cgggctgtcg gacgtcatca cttgcaggtg ttcgggagcg atgtcgttcg taatagccac
     8161 cgcctcgtcc agagactctg caagcagcac gcgcccgttg ttcctccagc tttggaaagc
     8221 gaattccccg gtggaaagga gcggcgcctg cgtttcgacg gcgctcataa cggattcacc
     8281 gagccgcctg cttgtcgtca caagtacggt ccacgaatgg gggtcgtgct cgcagcgcgc
     8341 aagagcgtcg gcggcaaccc actccgggtt cgccgattcg tcggcgagga tcaccacttc
     8401 gcttgggcct gcaagcatat cgatccctac tgccccggaa acctgtcgtt ttgcctcggt
     8461 aacatacctg tttccagggc cgacgattat gtcaacacct ttgatcgttt cggttccgta
     8521 ggcgaaagcg cctatcgcct gcgcgccacc catacagtag acctcgtcca ctcctgcgat
     8581 atccattgcg acaagaactg ccgggtgaat tccgtcccgt cctttgcccg ccggagaaca
     8641 cgccgctatc cgccgaattc cggcaacctt cgccggaata gccgacatca acgcggaaga
     8701 cggaagaggg tatctccccg caggaacata acaaccgcag gattccatgg ggagaagccg
     8761 atgcccgagc gtcactccgg gagaaatctc gtaccgaagc tcctgcatac agcttttctg
     8821 gcgaagtgca aagttgcgga ttcgttccgc ggcaaattca agcgtcttca ccgtcgaagg
     8881 cgctactttc tcataggcgg agcgcatctc ctctttccct atacgaagag aggaaggacg
     8941 gacaccgtcg aacttttccg tataggcgag aacagcctcg tctccgctgt cgcggacact
     9001 cttgaggata ctgcccacta cagattcgac agaacattc
//
LOCUS       DAOTIL010000012         8692 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminobacteriaceae bacterium isolate AMR_MDS_3026
            NODE_9708_length_8692_cov_3.521246, whole genome shotgun sequence.
ACCESSION   DAOTIL010000012 DAOTIL010000000
VERSION     DAOTIL010000012.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868050
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminobacteriaceae bacterium (wastewater metagenome)
  ORGANISM  Aminobacteriaceae bacterium
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae.
REFERENCE   1  (bases 1 to 8692)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8692)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:19:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,224
            CDSs (total)                      :: 3,198
            Genes (coding)                    :: 3,184
            CDSs (with protein)               :: 3,184
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8692
                     /organism="Aminobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9708_length_8692_cov_3.521246"
                     /isolate="AMR_MDS_3026"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:3034609"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..385
                     /locus_tag="P5201_00505"
     CDS             <1..385
                     /locus_tag="P5201_00505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005659338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="transketolase C-terminal domain-containing
                     protein"
                     /protein_id="HRV97042.1"
                     /translation="VVKEGSDVTLVSYSMTMNLCVKAAELLAKDGISAELIDLRTISP
                     LDKETILESVAKTTRLAIAHEAVKQGGVGAEIAALVAEEAIDCLDAPIVRVGAPFTPV
                     PFARPLEQAYRITPEKIAAAVRAMF"
     gene            395..886
                     /locus_tag="P5201_00510"
     CDS             395..886
                     /locus_tag="P5201_00510"
                     /inference="COORDINATES: protein motif:HMM:NF015444.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HRV97043.1"
                     /translation="METERVSSAAAEIGRTILVRLAPGEDVFEGLRTVCERHGLRSGR
                     IATMIGSLRTASLVCVTPDPDAPGRVVYLEPRTIEGPLELIAVQGVFGWDDDKDELSI
                     HLHAQVAGEDLRPFAGHLADTGKNRILATAEIVLDVFEGAELRRSFDEETGFTLFKIF
                     PKL"
     gene            1022..2095
                     /locus_tag="P5201_00515"
     CDS             1022..2095
                     /locus_tag="P5201_00515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005659340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_component: GO:0031317 - tripartite ATP-independent
                     periplasmic transporter complex [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_function: GO:0005488 - binding [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DctP family TRAP transporter solute-binding
                     subunit"
                     /protein_id="HRV97044.1"
                     /translation="MKKLVALLILVTALCAFSVPAFAAYKAEYKLDIVPSLTTSWGMG
                     AVYFTELVKERSGGKINIKVYPGSQLTVGAQTNAFMLVRNGTIDFAVQSSINYSPQII
                     ELNLFALPFFIANQPDKYAALDAVKAGKSGEMVVKAVEDKGVKFLAWGENGFRELTNS
                     KKEIRTPQDMEGMKIRVVGSPIFLDAFRAIGANPVTMNWSDTMAAIQQGVVDGQENPI
                     STFYPVKMHEYHKFVTDWHYVVDPTLYVVNPGVWKSFSEEDQKMIQEAAVEAGKYMIA
                     LARISLDDGTAHKYLESIGKLPEITDWYKELAKVGMTVSILSAEETKAFADKTAPVVK
                     EWRSKIGDAIVDAAEADMAAVAK"
     gene            2277..2747
                     /locus_tag="P5201_00520"
     CDS             2277..2747
                     /locus_tag="P5201_00520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013048966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter small permease"
                     /protein_id="HRV97045.1"
                     /translation="MVKKLFDNFEELLGSLILIVMAVVTFANVITRYFIFYALSFTEE
                     ITIYLFVWLVMLGTAIAFKKGANLSMTFLYDKLPRKGKKFCFYVSAGMSLVFFGLLAY
                     LGYVQVMDEIDLAVTSPSLAIPIYYYTIALPVFSALIFIRVLQATMETLRKNEF"
     gene            2760..4058
                     /locus_tag="P5201_00525"
     CDS             2760..4058
                     /locus_tag="P5201_00525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009164908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0031317 - tripartite ATP-independent
                     periplasmic transporter complex [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter large permease"
                     /protein_id="HRV97046.1"
                     /translation="MESLLREPAFWTIVLFLVPLLLRVPIAVALGSAAIFVVWNWDLG
                     YQMLSYSFFSGIAKFPLLAIPFFILAGVIMEKVGIAERIIALIKQLVGDMTGGLAVAT
                     VIVAAFWGAVSGSGPATVAALGLILIPGMAEAGYDKAFATAVVSVSAGLAIIIPPSIS
                     FIVYGDIMQQSVGTLFAAGVVPGIVVAVFLCVTVWLISRKHGYRGAPRGTSKEVLFAL
                     KDAFWGLFTPVIILGGIYGGVFTPTEAAAVAVFYGLFVGVFIYRSLTLRILYDILRET
                     VAGTSVVMIVVTCAGLYSWLGATVGLIDKIAALLLGISENPLVILLMINIILLFAGML
                     LDAISIAYVFLPILAPVIVTFGWDPIWFGVMMTVNMAIGQVTPPVAVNLYVGARISGL
                     TMEQITRPVIPLLVASIIALAVITIFPGITLYLPRLLGMM"
     gene            4129..5418
                     /locus_tag="P5201_00530"
     CDS             4129..5418
                     /locus_tag="P5201_00530"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013048964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoamide acetyltransferase family
                     protein"
                     /protein_id="HRV97047.1"
                     /translation="MASPVTMPKLGLTMNTGVVSQWKKKEGEPVRKGEILMVVATDKL
                     TFDVESPSEGVLLKVLVPEGKDVPVGEVLAYIGAAGETVAGAAPEMPAAAPAAEEKKA
                     AAPSEVRSAPAGGIRATPLARKTAREAGIDLSLVAGSGPSGRIVRKDVDAAAASGGGA
                     VKASPVAAKMAAELGVDMASMRKDGRVMKEDVLRAAAVPEAEDVRIPLTPMRKVIAER
                     MSLSNSTVPSVTYTLEADFSALVAFRNKVKDEAAKKGVKISYNHILMKICAAALKELP
                     LANASFEGDAIVMHGNVNIGLAVSVEGGLLVPNVKAVQSKSLLRIAEETEHLVEQARG
                     GSLSLDAMQGGTFTITNLGMFGMQSFTPIVNPPEACILGVNAIVEKPVVVNGEIVVRP
                     MSALCLVADHRILDGAEAARFFARIKELVENPWLLLL"
     gene            5437..6840
                     /gene="lpdA"
                     /locus_tag="P5201_00535"
     CDS             5437..6840
                     /gene="lpdA"
                     /locus_tag="P5201_00535"
                     /EC_number="1.8.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013048963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004148 - dihydrolipoyl dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA];
                     GO_process: GO:0045454 - cell redox homeostasis [Evidence
                     IEA];
                     GO_process: GO:0051068 - dihydrolipoamide metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyl dehydrogenase"
                     /protein_id="HRV97048.1"
                     /translation="MEARKKVAIIGAGPGGYVAAIRAAQLGAEATVIEKNALGGVCLN
                     VGCIPTKVLLHTAELFTAAKEGAMIGLKADNVAVDWDGLMARKKAVVETLVGGVGTLL
                     RSNEVSIVEGEASFVTPKEIAVTKKDGSKESVSADAVIVATGSEPVVPPVPGFDLDGV
                     MTSTGALSLESLPKSVVLVGGGVIGMEFASIFSSFGAKVTVVEMLPEILPMLDEEIVQ
                     ILKGVLLRKGVSFHTNSKVVGVEKKGKELAVRVETPAGPLVLDAEKVLVSVGRRSVTK
                     GLGLDRLGIAFDRARVKVNSRMETSVKGIYAIGDCASHIMLAHVASREGEVAAENIMG
                     HAVDMDYKTVPSAVYTAPEIASVGLSESEAKSRGNRVKVGRFPLMANGKSLIMNESDG
                     MVKYVVDEKYDEILGVHIIGPRATDLIAQGGLALRLEATVDEIITTIHAHPTVGEALL
                     EGAMAVQGRTISLPKKA"
     gene            7035..8480
                     /locus_tag="P5201_00540"
     CDS             7035..8480
                     /locus_tag="P5201_00540"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HRV97049.1"
                     /translation="MFSMAAGPLSVFVFSALLLTCVFLLYERRRLRKIVNEEALAKSA
                     LGESIVRFHMIVESSAAATYVFRGGRFVMVNAAMEAITGYSREELLSMDSLEHIHPDH
                     RPSALERVAARERGEAVSTRYELKLLTKTGEVRWVDFTARTVLFDGVLCTVGSAFDIT
                     GRKRVEDALRESEAKYRFLTESMKDVIWTLDAETLMFVYVSPSVERLRGYTPEEIMAE
                     PLDAALTPESAALVHRLIDQETEKFKMREERGDAVFHTEEVEQPRKDGTTVWTEVVTV
                     YWRNPQNGRIEIHGVTRDISERKEVEAKMAHMAQHDPLTGLPNRALFSDRLERALAAS
                     RRSGIPTALMFLDLDKFKPVNDTYGHAIGDVLLQQAAERMKSALRESDTVGRIGGDEF
                     VVLLPAVERVEDALHVAEKLLEALRKPFFIEGRSVDVSCSIGIAIHPEDGRDEIELAK
                     NADTAMYWAKRDGGDCVRMFRTASDVSAAVL"
     gene            complement(8492..>8692)
                     /locus_tag="P5201_00545"
     CDS             complement(8492..>8692)
                     /locus_tag="P5201_00545"
                     /inference="COORDINATES: protein motif:HMM:NF021921.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoate protein ligase C-terminal
                     domain-containing protein"
                     /protein_id="HRV97050.1"
                     /translation="NVKEGVIRQARFYGDYFGAENREEIERLLEGRFYREDALREALE
                     GFPLERSFKGAGTEEFLTFLMS"
BASE COUNT         1686 a         2498 c         2729 g         1779 t
ORIGIN      
        1 ggtcgtaaaa gagggaagcg acgtgacgct cgtttcgtac tcgatgacca tgaatctctg
       61 cgtcaaagcg gcggagttgc tggcgaagga cggcatcagc gccgagctga tcgacctccg
      121 gacgatttcg cctctggaca aggagacgat cctcgagtcg gtggcgaaga ccacccgcct
      181 cgccatcgcg catgaagctg tcaagcaggg cggcgtgggc gcggagatcg cggctctcgt
      241 cgccgaggag gcgatcgact gccttgacgc ccccatcgtc cgcgtcggcg ctccgttcac
      301 cccggttccc ttcgcgcgcc cgctggagca ggcgtaccgg attactccgg agaagatcgc
      361 cgccgccgtc agggcgatgt tctaggaaga ggccatggag acggaacggg tttcgtcggc
      421 ggctgcggaa attggacgga cgattctcgt tcgtctcgct ccgggagagg acgtcttcga
      481 ggggctgcgc accgtatgcg aacgccatgg actccggtcc gggcgaatcg caacgatgat
      541 agggagtctg cgcaccgcga gcctcgtctg cgtgactcct gatccggacg ctccggggcg
      601 ggtggtctat ctcgaacccc ggactatcga gggcccgctt gaactgatcg cggtacaggg
      661 agttttcggc tgggacgatg acaaagatga actttcgatc catctgcacg cgcaggtggc
      721 cggggaggat cttcgtccgt tcgcggggca tctggccgat acggggaaga accggattct
      781 cgccacggcg gagatagttc tcgacgtttt tgaaggcgcc gaactcaggc gctcgttcga
      841 cgaggagaca gggttcacgc ttttcaagat ttttccgaaa ctgtaatcat acgttacaat
      901 acaaggacaa tgtcgctgga acaaggaggt gatcactccg ctaccgaagg aagtgtggaa
      961 ggaaaggcgc tattccgaac atgggaagtt gtaaaaccac attcaggagg taaagaagaa
     1021 gatgaaaaaa ctcgttgcgc tgctcatact tgtaacagct ctgtgcgcgt tctccgttcc
     1081 ggcctttgcg gcctacaaag ccgagtacaa actcgatatc gtccccagcc tcaccacatc
     1141 ctggggtatg ggcgccgtct atttcaccga acttgtgaag gagagaagcg gcggcaagat
     1201 caacatcaaa gtgtatcccg gctcccagct taccgtagga gcgcagacga acgcgttcat
     1261 gctcgtccgg aacgggacga tcgacttcgc ggtgcagtcc tccatcaact actctccgca
     1321 gatcatcgag ctgaacctct tcgcccttcc gttcttcatc gcgaatcagc ccgacaagta
     1381 cgccgcgctt gacgcggtca aggcggggaa gtccggagag atggtcgtca aggccgtcga
     1441 ggacaagggt gtgaagttcc tcgcatgggg agagaacggc ttccgcgaac tgacgaattc
     1501 gaagaaagaa attcgcactc ctcaggatat ggaaggaatg aagatccgcg tggtcggcag
     1561 ccccatcttc ctcgacgcgt tccgggcgat cggcgccaac cccgtcacga tgaactggag
     1621 cgataccatg gcggccattc agcagggcgt cgtggacggc caggagaacc ccatcagcac
     1681 gttctacccc gtgaagatgc atgagtacca caagtttgtc accgattggc attatgtcgt
     1741 cgaccccacg ctctacgtcg tgaaccccgg cgtgtggaag tccttctccg aagaggatca
     1801 gaagatgatt caggaagccg ccgtggaggc aggcaagtac atgatcgcgc ttgcccgtat
     1861 cagcctggac gacgggaccg cgcacaagta cctggagagc atcggcaagc tgcccgagat
     1921 caccgactgg tacaaagagc tcgccaaggt cggcatgacc gtgagtatcc tgtcggctga
     1981 agagacgaag gcgttcgccg acaagacggc gcccgtcgtc aaagagtggc gttccaagat
     2041 cggcgacgcc atcgtcgacg ccgccgaggc ggatatggct gctgtggcga agtagacgtc
     2101 ttttgtttgc gacctcgggg ggacggggct ccccccgagc cctcggctcg tttgcccggg
     2161 ggccttctct tccttcctcc ccgcacgctt cttcgtttgt cttcgaggag ggcgtctgcg
     2221 ggacgcgtgg gggaggaaat cacagagtct tttttcttca gaagggattg gcctaaatgg
     2281 taaaaaaatt attcgataac ttcgaggaac ttctcggctc tctcattctt attgtgatgg
     2341 cggtggtcac gttcgccaac gttattaccc gctacttcat cttttatgcg ctttcattca
     2401 cagaagagat caccatctac ctttttgtct ggctggtgat gctgggaaca gcgatcgcct
     2461 tcaaaaaggg cgccaacctg agcatgacct ttctctacga caagctcccc cgtaagggaa
     2521 agaagttctg tttctacgtt tccgccggca tgtcgctcgt gttcttcggt cttctggcgt
     2581 acctggggta tgttcaggtg atggacgaga tcgacctcgc cgtcacctcg ccgtcgctcg
     2641 ccataccgat ctactactat acgatcgctc tgcccgtctt ctccgcgctc atcttcatcc
     2701 gcgttctgca ggcgactatg gaaacgctcc gcaaaaacga gttttaggag ggacgagaga
     2761 tggagtcttt gcttcgtgaa ccagcatttt ggacgatcgt tctctttctg gtccctctcc
     2821 tgttgagggt ccccatcgcc gtagccctcg gttcggccgc catcttcgtg gtctggaact
     2881 gggatctcgg gtatcagatg ctttcgtaca gcttcttctc cggcatcgcg aagtttcctc
     2941 ttcttgcgat tcctttcttc attcttgccg gagtcatcat ggaaaaagtc ggcatcgccg
     3001 agcggattat cgccctcatc aagcagctcg tgggcgatat gaccggcggc cttgccgttg
     3061 cgacggtcat cgtggccgcg ttctggggcg ccgtgagcgg ctccggtccg gcgacggtgg
     3121 cggctctcgg cctcatcctc attcccggca tggcggaggc tgggtatgac aaggcgttcg
     3181 ccaccgcggt cgtctccgtg tccgcggggc ttgcgatcat cattccgccg agtatctcct
     3241 tcatcgtgta cggcgacatc atgcagcagt cggtgggcac gctcttcgcc gccggagtcg
     3301 taccgggcat cgtcgtcgcc gttttcctct gcgtcacggt gtggctgatc agtagaaaac
     3361 acggctacag gggcgctccg aggggtactt ccaaggaagt tctttttgcg ctgaaggacg
     3421 ctttctgggg gctcttcaca ccggtgatca tcctcggagg catttacgga ggggtcttca
     3481 cgcccacgga ggccgcggcg gttgcggtgt tctacggtct gttcgtcggc gtcttcatct
     3541 accgaagtct cacgctccgc attctctacg acatactccg ggagacggtc gccgggactt
     3601 ccgtggtgat gatcgtggtc acctgcgcag ggctttactc ctggctcggc gctacggtgg
     3661 gactcatcga caagattgcg gcgcttcttc tgggcatctc cgagaatccg ctggtcattc
     3721 tgcttatgat caacatcatc ctgctcttcg cagggatgct gcttgacgcg atctcgatcg
     3781 cctacgtctt cctcccgatc ctggcgccgg ttatcgtcac cttcggttgg gatcccatct
     3841 ggttcggcgt gatgatgacc gtcaacatgg ccataggcca ggtcaccccg ccggtggcgg
     3901 tgaacctcta tgtcggcgcg agaatcagcg gactcacgat ggagcagatc acccgaccgg
     3961 tcataccgct cctcgtggcg tcgatcattg cgcttgccgt cattaccata ttcccgggga
     4021 tcacgctcta cctgccccga cttctgggca tgatgtagcg cggacagcgt atctgcctgt
     4081 cgttgtacag cgtatgttct ttcgttcctc aaaaggaggg gtttttgaat ggcttcaccc
     4141 gtaacaatgc ccaagctcgg gctgacaatg aacacagggg ttgtctccca gtggaagaag
     4201 aaggagggag agcccgtccg gaaaggcgag attctgatgg tcgtcgccac ggataagctg
     4261 acgttcgacg tggaatcgcc ctccgaaggc gttctgctga aagtcctcgt ccctgaagga
     4321 aaggatgttc ccgtaggcga ggtgctcgcc tacatcggcg ccgccggcga aacagttgcg
     4381 ggcgcagctc cggaaatgcc cgcagcggcg cccgcggcgg aggagaagaa ggccgccgct
     4441 ccttccgagg ttcgcagcgc tccggcgggc ggaatccgcg ccacgccgct tgcccgtaag
     4501 acggcgcgtg aggcggggat tgacctctcg ctcgtcgccg gttcgggacc gtcgggtcgt
     4561 atcgtccgaa aggatgtgga cgccgcggcc gcctcgggcg gcggcgctgt caaggcaagc
     4621 ccggtcgcgg cgaaaatggc tgcggagctg ggcgtcgaca tggcttccat gcgcaaggat
     4681 ggtcgtgtga tgaaggagga cgttcttcgt gccgccgccg ttccggaggc ggaggatgtc
     4741 cgcattccgc tgacgccgat gcggaaggtc atcgccgagc ggatgagcct gagcaactcc
     4801 acggttccct cggtgacgta cacactcgaa gcggacttct cggcgctcgt tgccttcaga
     4861 aacaaggtga aggacgaagc ggcgaagaag ggcgtcaaga tctcctacaa tcatatcctg
     4921 atgaagatct gcgccgccgc gctcaaggag ttacctctgg cgaacgcctc cttcgaaggg
     4981 gatgccatcg tgatgcacgg caatgtgaac atcgggctcg ccgtctcggt cgagggaggg
     5041 ctgctcgttc ccaacgtgaa ggcggtgcag tcgaagagcc ttcttcgcat cgcggaagag
     5101 acggaacatc tcgtcgaaca ggcgcggggc ggttcgctct ccctcgacgc gatgcagggc
     5161 ggcacgttca ccatcacaaa tctcggcatg ttcggcatgc agagcttcac accgatcgtg
     5221 aaccctccgg aggcgtgtat cctcggagtg aacgccatcg tggaaaaacc ggtcgtcgtg
     5281 aacggcgaga tcgtcgtccg cccgatgtcg gcgctctgcc tggtagccga ccaccggatt
     5341 ctcgacggcg cggaggccgc gcgtttcttc gcccgtatca aggagctcgt cgagaacccc
     5401 tggctccttc ttttatagag aggacgggga gtgtccatgg aagcgcgaaa aaaagtcgca
     5461 ataatcggcg cggggcccgg cgggtacgtc gcggccatca gggcggcgca gcttggcgcc
     5521 gaggcgaccg tcatcgaaaa gaacgccctc ggcggcgtct gtctcaacgt cggctgcatt
     5581 ccgacgaagg tgctgctgca tactgcggag ctgttcaccg cggcgaagga gggcgcaatg
     5641 atcggcctga aggccgacaa tgtggcagtc gactgggatg gacttatggc acgaaagaag
     5701 gccgtcgtgg agacgctcgt cggcggcgtc ggaacgctgc tgcgcagtaa cgaagtcagc
     5761 atcgtcgagg gcgaggcgtc gttcgtcacg ccgaaggaaa tcgccgtgac gaagaaggat
     5821 ggctcgaagg agtcggtttc cgccgacgcc gtcatcgtgg cgacaggttc ggagccggtg
     5881 gttccgcctg ttcccggatt tgacctcgac ggcgtgatga cgagcaccgg agcgctctct
     5941 ctggagagcc ttccgaagtc ggttgtcctc gtgggcggcg gcgtcatcgg catggagttc
     6001 gcctcgattt tttcatcttt cggcgcgaag gtgacggtgg tcgagatgct cccggagatt
     6061 cttccgatgc tcgacgagga gatcgtccag attctcaagg gcgtccttct gagaaagggc
     6121 gtttccttcc ataccaactc caaggtcgtc ggtgtggaga agaaggggaa agagctcgcc
     6181 gtcagggtgg agacgcctgc gggcccgctc gtcctcgacg cggagaaggt gctcgtctcc
     6241 gtcggccgcc gttccgttac gaaggggttg gggctcgacc gcctcgggat tgccttcgac
     6301 agggccagag tgaaggtgaa ctcacggatg gagacgtccg tgaagggaat ttacgccatc
     6361 ggggactgcg cgtcgcatat catgctcgcg cacgtcgcct cccgcgaggg agaggtcgcg
     6421 gcggagaaca tcatggggca cgccgtcgac atggactaca agaccgttcc cagcgcggtg
     6481 tacaccgctc ccgagatagc ctccgtcggt ttgagcgaga gcgaggcgaa gagcaggggg
     6541 aatcgcgtca aggtcggccg cttcccgctg atggccaacg gaaagagcct gatcatgaac
     6601 gagagcgacg gcatggtgaa gtacgtggtt gacgagaagt acgacgagat cctcggcgtc
     6661 cacatcatcg gcccgcgagc caccgacctc atcgcgcagg gcgggctggc gctccggctg
     6721 gaggcgacgg tggacgagat catcacgacc atccatgcgc accctacggt cggcgaggcg
     6781 ctgcttgagg gggcgatggc agtccaggga cggaccatat cgctgccgaa gaaggcgtag
     6841 tatcccagaa caatctgctg atgaaggcgg cgttaccggt tgatccggaa cgccgctttc
     6901 tgcgtttcct gggaaaagtt cgttcatgag aaaacatgag aaaaaaagag cgacggagga
     6961 agcgaggtat gctatcctta gtggtgtttg gaaattatga agccgttttc cctggtttcg
     7021 cgggaggaaa atatatgttc tcaatggccg ccggtcctct ttcagttttt gttttttctg
     7081 cgctgcttct tacctgtgtc tttttgctgt atgaaagacg tcgtctgcgg aagatcgtca
     7141 acgaagaggc gcttgcgaag agcgctctcg gagagagtat cgtccgtttc cacatgatcg
     7201 tcgagagttc cgccgccgcg acgtatgttt ttcgcggagg gcgttttgtc atggtgaacg
     7261 ccgcgatgga ggcgatcacc ggctactccc gcgaggagct gttgtcaatg gattctcttg
     7321 aacacatcca ccccgatcat cgtccttcgg cgctggagcg ggtcgctgcc cgggagcgcg
     7381 gggaagctgt ttccacacgc tacgaactga agctgctcac gaagacaggt gaagtgcggt
     7441 gggtcgattt caccgcgaga accgttctct tcgacggtgt tctctgcacc gtaggaagcg
     7501 ccttcgacat tacgggacgc aagagggtgg aggatgcgct cagggagagc gaggcgaaat
     7561 acagatttct cacggagagc atgaaggacg tcatctggac gctcgacgcg gagacgctta
     7621 tgtttgtgta tgtcagcccc tccgttgagc ggcttcgagg atatacgccc gaggagatca
     7681 tggccgaacc tctcgacgcc gctctgacgc cggagtcggc ggccttggtc cacaggctca
     7741 tcgatcaaga aacggagaag ttcaaaatgc gggaagaacg cggagacgcg gtgtttcata
     7801 ccgaggaggt cgaacagccc cgcaaggacg gaacgactgt gtggacagag gtcgtgaccg
     7861 tctactggcg caacccgcag aacgggcgaa tcgagattca cggcgtgacc cgcgatatct
     7921 ccgagcgtaa ggaggtcgag gcgaagatgg cccatatggc gcagcacgac ccgctcaccg
     7981 gtctccccaa ccgggcgctc ttttccgacc ggctggagcg tgcgctggcc gcgtccagaa
     8041 gaagcggaat cccgacggcg ctgatgttcc tcgaccttga caagttcaag ccggtgaacg
     8101 atacgtacgg gcacgcgatc ggcgatgtgc tgcttcaaca ggcggccgaa cgcatgaaat
     8161 cggcgttgcg cgaatccgac acggtggggc ggatcggcgg cgacgagttc gtcgtgctgc
     8221 tccctgcggt ggagcgggtg gaggatgcgc tccacgtggc ggagaagctc ttggaagccc
     8281 ttcgcaaacc attcttcatc gaagggcgtt ccgtcgacgt ctcgtgcagc atcggcattg
     8341 ccattcatcc ggaggacggc cgcgacgaga tcgaactcgc aaagaacgcc gacacggcca
     8401 tgtactgggc gaagagggac ggaggtgact gcgtccgcat gttccggacc gcttccgacg
     8461 tttcggctgc ggtcctctga cgatacgatg ctcaggacat caaaaaagtc aggaactctt
     8521 ccgtgcccgc tcccttgaaa gagcgctcca gcgggaaccc ttccagcgcc tcgcggagcg
     8581 catcttccct gtagaagcgc ccttcgagca gcctttcgat ctcctccctg ttctccgcgc
     8641 cgaagtagtc gccgtagaat cgcgcttgcc gtatgacgcc ctccttcacg tt
//
LOCUS       DAOTIL010000013         8536 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminobacteriaceae bacterium isolate AMR_MDS_3026
            NODE_9947_length_8536_cov_3.485320, whole genome shotgun sequence.
ACCESSION   DAOTIL010000013 DAOTIL010000000
VERSION     DAOTIL010000013.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868050
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminobacteriaceae bacterium (wastewater metagenome)
  ORGANISM  Aminobacteriaceae bacterium
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae.
REFERENCE   1  (bases 1 to 8536)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8536)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:19:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,224
            CDSs (total)                      :: 3,198
            Genes (coding)                    :: 3,184
            CDSs (with protein)               :: 3,184
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8536
                     /organism="Aminobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9947_length_8536_cov_3.485320"
                     /isolate="AMR_MDS_3026"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:3034609"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..378)
                     /locus_tag="P5201_00550"
     CDS             complement(<1..378)
                     /locus_tag="P5201_00550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013047776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HRV97051.1"
                     /translation="MLKVKEAMPKDVGHSIARISPEDMAALGLSEGQIVEIEGKRRTP
                     VRILSCDSDLVASGGAKGVVQIDGITRDNAQASIDDKVSVHTTTHHFAGSVTLRPLTS
                     TALLEKERDAAYIRSLLEGLPVLT"
     gene            complement(496..1707)
                     /locus_tag="P5201_00555"
     CDS             complement(496..1707)
                     /locus_tag="P5201_00555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013047777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HRV97052.1"
                     /translation="MKFSERVLAMQSSPVRRLLPYAEEARAKGKKIYPLNIGQPDIKT
                     PESFLKAIKDFSQEVIAYATSQGNNDLRDAISRYYKEFNIPIEREDLIVTNGGSEALL
                     FAFMTMCDPGDEVLIPEPFYANYNAFAQSVNVKVVPITTKAEEGFHLPPKEQVEALVT
                     PRTRAILVANPGNPTGTIYTRDEMDMLADLALRHNLYIVADEVYREFVYDGLKYTSFG
                     HLPEIQDRVIIVDSVSKRYSACGARIGCIACKNKDFMAQVLKIAQGRLCVPTLEQVGA
                     TALYSTPKSYLDEVNKEYQSRRDTLYAALKKMPGVICSEPKGAFYVMVKLPVDDAEKF
                     IIWMLQNFDVDGETVMASAGEGFYATPGLGKQEFRLAYVLKNDELVKAMDILKVALEA
                     YPGRIEPVAAR"
     gene            1857..3065
                     /locus_tag="P5201_00560"
     CDS             1857..3065
                     /locus_tag="P5201_00560"
                     /EC_number="5.4.2.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009202360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-bisphosphoglycerate-independent
                     phosphoglycerate mutase"
                     /protein_id="HRV97053.1"
                     /translation="MKRMDILKSLATDNGKKMILLVMDGIGGLPGPEGKTELEAAATP
                     NMDALARKSELGLLDMVDVGITPGSGPGHLSLFGYDPMEFSIGRGILEAMGVGALVGP
                     GDVCARGNFCTRSDDDVITDRRAGRIPTEKSALVVKKLAESIKEINGVRVTFYPGKEH
                     RFVVVFSGEGLSENVADADPQQEGSPMRWARPTVSDGERMADVANAFIKKVAEVLKGE
                     APANGCLLRGFSSAPDIPLLGDVYKIKPVAVATYPMYKGLAKLVGMNVVEAGETLDKL
                     FDSVGRLWSDYDYFYVHVKYTDSRGEDGDFEGKKKVVETVDALLPRLLALSPDVLVIT
                     GDHSTPSVMSGHSWHPVPFLLASEFVRGGDSKSFDERECRIGAGGRIEAKKLLGLMLA
                     HARRLDKFGA"
     gene            3099..4130
                     /locus_tag="P5201_00565"
     CDS             3099..4130
                     /locus_tag="P5201_00565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005659445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HRV97054.1"
                     /translation="MEEQQICTIGEIRRMSKDEKFKVLGVVVKSSTRKDKNGKNYWDL
                     GIMDSEGTIEGKVWSNAQWWDRRDAPLLVAEPAESEILRDLTGKTVGLVGQVTEFRGQ
                     QQYNFNAVSLVNQEKYPPHQYVQRSPVPEESLERAFLELLEVCEGPVGDFLDFVFFKK
                     NLWERFKTCPAAVSHHHAYVGGLLEHTIAVARTAKNIVEAGISGGYEADVAVTLAGAL
                     LHDLGKMEAYRLVPAPVMTVAGTFHDHIVIGYSMFESLAREFNLDERLFLAIGHIIVS
                     HHGCREYGSPVLPATPEAMAVSAADEMDFRLFCWKHSVDQMEDGREISDFNPSAQRRF
                     WKWELNNGV"
     gene            4120..5049
                     /locus_tag="P5201_00570"
     CDS             4120..5049
                     /locus_tag="P5201_00570"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HRV97055.1"
                     /translation="MGYKLHISPSDDGRRLDKTIRSIWPALPLSVVMKGIRTGAVRLD
                     GKKAACDVRITAGQELYVPWEGPAEREEGFRYRRTLPILFEDERLLAVNKPENLLVQP
                     DRKDQDNVLDRVKYMRSLEGAEERAYAVHRLDRNTTGVLLVALSGVSLRILQDAFRAR
                     EVRKTYLAVVSGVPPAKGEIDAPLLKDPASNTVKVDPSGKEALTRYRLLAAGGDVSLV
                     EIDLVTGRSHQARVHMASIGHSILGDIRYGSENVNAKWRKRGIRRPLLHSYQVVFGSL
                     LGELESLSGMRIAAPLPEDMGELLRSRGWKPPV"
     gene            5241..5414
                     /locus_tag="P5201_00575"
     CDS             5241..5414
                     /locus_tag="P5201_00575"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRV97056.1"
                     /translation="MEAEFQKIEQDGVVLWVPSFMEFKNNEILIVQRGFLWSAYLMLV
                     GVLSESEESCCNS"
     gene            5608..6885
                     /locus_tag="P5201_00580"
     CDS             5608..6885
                     /locus_tag="P5201_00580"
                     /EC_number="1.4.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006584084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Glu/Leu/Phe/Val dehydrogenase"
                     /protein_id="HRV97057.1"
                     /translation="MSVVKRTSSNVLLDTALKNFYAAAEEMGLEEGLIDILSHSERKT
                     SVSIPVEMDDGSVKVFDGYRVLHSSAIGPGKGGVRFHPEVNVEECEALAMMMTWKCSL
                     AGIPYGGGKGGVSCEPLELSKKEKERISRTWAARMAPVIGAWTDVPAPDVNTGGPEMV
                     WFMDTISKMKNQLEPAIFTGKPIPLWGSKGRTAATGYGVATCALELLKNLGKDPKAIS
                     VSIQGFGNVGTYAALTMVNAGSKVVAISDITGTYFAEKGLDIKKAIDHVNNHPKHLLE
                     GFTCDGCQKLDLPEALFVNCDILIPAALEGVINGKNADKVKAKYIVEAANGPVTPEGD
                     AILDAKGVLVVPDFLANSGGVIGSYFEWAQDLGGFFWSEEEYNERLIRIMKDNFQRVW
                     GYAKDKNVKMRRAAFMAAIQRVADAVRMRGVFL"
     gene            7082..8311
                     /locus_tag="P5201_00585"
     CDS             7082..8311
                     /locus_tag="P5201_00585"
                     /EC_number="1.4.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006584283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Glu/Leu/Phe/Val dehydrogenase"
                     /protein_id="HRV97058.1"
                     /translation="MFLQQLEQAVPYVTVEPEYLEILRHPKEVLELSLPVRLDGGGVK
                     VFKAWRSHHNNALGPYKGGVRYHAEVSRDEVVALSSWMTIKCAIAGLPYGGGKGGISV
                     DIRELSRNELETLSRAYAFGLARFTGTDYDIPAPDVYTNPQIMSWFLDTYEKVKGWSE
                     SSAYTGKPPVLGGSLGRSDATGKGGVFILREGLAKLGLLGKKDLKVGIQGYGNAGTRA
                     HRFVEDDLGLTVVAVSDSGAALYNPKGLPYREVSKHKNNTRSLKGFQGAEELFPDLLF
                     GLDMDILILAALGGAVDAQNAPDIKAHVLLEIANGPVTPDAEALLTDKGVTIIPDVLA
                     NAGGVIVSCFEWIQGRSGDVWTEEYVFQKLDERLTLSFKDVMKIAEDRKISARKAAYV
                     KAVGRIVDAMRFRGIWP"
BASE COUNT         1945 a         2113 c         2535 g         1943 t
ORIGIN      
        1 ggtaagaacg ggaaggcctt caagaaggga acggatatac gcggcgtcgc gctctttctc
       61 cagcagcgcc gtagaggtaa gcggacggag cgtaacgctt cctgcgaagt ggtgcgtcgt
      121 cgtatggacg gacaccttgt cgtcgatact cgcctgcgcg ttgtcccttg tgataccgtc
      181 aatctggacc acgcctttcg cgcccccgga ggcgacaagg tcggaatcgc atgagagaat
      241 ccggacggga gtgcggcgtt tcccttctat ttcgactatc tggccttccg aaaggcccag
      301 cgcggccatg tcctcggggg agatgcgggc aatactgtgt ccgacatcct taggcatagc
      361 ttccttgact ttcagcatgt gtaacttcct ctcttcattg aatgttctgc ggaaaacata
      421 ataacatacc ctgcggccgc cgttcatccg gaccgcaggg tatggaaggg cggagaatcc
      481 gtgtttacgg aagaatcagc gagcggctac cggctcgata cgccccggat aggcttcaag
      541 agcgaccttg agaatatcca ttgccttaac aagctcatcg ttctttaaga cgtacgcaag
      601 acggaactcc tgctttccca gcccgggagt cgcgtagaat ccctcgccgg cggaagccat
      661 gacggtttcg ccgtcaacgt cgaagttctg aagcatccag atgatgaact tctccgcgtc
      721 gtcgacgggg agcttcacca tgacatagaa ggcgcccttc ggttccgagc agataactcc
      781 gggcatcttc ttcagcgcgg cgtagagcgt gtcgcgccgg gactggtact ctttgttcac
      841 ttcatcaagg tacgattttg gcgtactgta cagcgctgta gcgccgacct gttccagcgt
      901 gggaacgcag aggcggccct gcgcgatctt gagaacctgc gccatgaagt ccttgttctt
      961 gcacgcgatg cacccgatgc gggcgccgca ggcgctgtag cgcttcgaaa cagaatcgac
     1021 gatgatgact ctgtcctgta tttccggcag gtggccgaag cttgtgtatt tcagtccgtc
     1081 gtacacgaac tcccggtaga cttcgtccgc gactatgtag agattgtgtc tgagcgcaag
     1141 gtccgcgagc atatccatct cgtcgcgtgt ataaatcgtt ccggtcggat ttccagggtt
     1201 cgccacaagg attgcccgtg tccgcggcgt aacgagcgct tcgacctgct ccttgggggg
     1261 aagatggaag ccttcttcgg ctttggtcgt aattgggacg actttgacgt tcacgctctg
     1321 tgcaaacgcg ttataattgg cgtagaatgg ttcagggatg agcacttcgt ctccggggtc
     1381 gcacatcgtc ataaacgcga aaagaagggc ttcgctgccg ccgttggtga caataagatc
     1441 ttctctctcg attgggatgt taaactcttt gtagtagcgg cttattgcat ctctcagatc
     1501 gttgttcccc tgggaggttg cgtacgcaat aacctcctgc gaaaagtcct tgatagcctt
     1561 gaggaacgat tcgggtgttt tgatgtccgg ctgcccgatg ttgagaggat agatcttctt
     1621 ccccttcgct cgcgcctcct ccgcgtacgg cagtagtctg cgcaccggag acgattgcat
     1681 cgccaggact ctttcggaaa acttcacctt gatgaccccc ttgaattgaa attaagtgga
     1741 tggaccgctg aagaaaatga taacataaaa tgcagaggtt tttgcagaaa aaaacgagca
     1801 ctcgagacaa atcgatccat tagtgaaatg aaaacttttc gaaggtggcg agaaatatga
     1861 aaagaatgga tatattgaag agcctggcga ctgacaacgg caagaagatg attcttcttg
     1921 tcatggacgg tataggaggt cttcccggcc ccgaaggaaa aacggaactc gaagcagcgg
     1981 cgacgccgaa tatggacgcc ttggcgagaa agagcgaact cggccttctg gacatggtcg
     2041 atgtcggcat aactccggga agcgggcccg ggcatctctc cctcttcggg tacgacccga
     2101 tggagttctc catagggcga ggaattctcg aagccatggg cgttggcgcg cttgtaggtc
     2161 cgggagacgt atgcgcgcgg gggaactttt gcacccgttc cgacgacgat gtgatcaccg
     2221 acaggcgcgc ggggcgcatc cccaccgaga agagtgcgct tgtcgtgaag aaacttgctg
     2281 aatcgatcaa ggagattaac ggagtccgtg taacttttta cccgggaaag gaacatcgtt
     2341 tcgtcgtcgt cttctcggga gaggggttga gcgagaacgt ggccgacgcc gatcctcagc
     2401 aggaaggcag tccgatgcgc tgggcgcggc cgacggtttc agacggcgaa cggatggcgg
     2461 acgttgcaaa cgccttcata aagaaagtcg cggaggtgct gaaaggcgaa gctccggcga
     2521 acggctgtct tctgcgcgga ttttcatccg ctcctgatat cccgcttttg ggagacgttt
     2581 ataaaatcaa gcctgtggca gttgcaacct accctatgta caaggggctc gccaaactgg
     2641 tcgggatgaa cgtcgtcgaa gcgggcgaga cgcttgataa gcttttcgac agcgtggggc
     2701 gcctttggag cgactacgac tacttctacg tccacgtgaa atatacagat agtcgcggcg
     2761 aggacggcga ttttgaagga aaaaagaaag ttgtggaaac agtggacgcg ctcctgccgc
     2821 gtttgcttgc gctctcgccc gacgttcttg tcattaccgg agaccacagc acgccaagcg
     2881 tgatgagcgg ccattcctgg catccggtcc ccttcctgct cgcaagcgag tttgtccgcg
     2941 gaggcgactc gaagtcgttt gacgagcgcg aatgccgtat cggcgcaggc gggcgcatag
     3001 aggcgaaaaa acttctcggc ctgatgctgg cgcacgcccg caggctcgat aaattcggcg
     3061 catagcggcg ccgacgtatg cgtacatgag gtgattgagt ggaagaacag cagatttgca
     3121 cgatcggcga aatacggcgg atgtcaaagg acgagaagtt caaggtgctt ggcgtcgttg
     3181 tgaaatcgtc gaccaggaaa gataagaacg ggaagaacta ctgggatctc ggtatcatgg
     3241 attctgaagg gacgattgag ggaaaggtgt ggtcgaacgc gcagtggtgg gacaggagag
     3301 acgctcccct gctcgtcgcg gaaccggctg aatcggagat tttacgcgat cttacgggaa
     3361 agactgttgg tcttgtcggt caggttacgg agttcagagg acagcagcag tacaatttca
     3421 atgcggtttc acttgtcaat caggaaaaat acccgccgca tcagtacgtg cagcgttctc
     3481 ccgtgccgga ggagagcctt gaacgcgcat tcctggaact gctggaagtg tgcgaaggac
     3541 ccgtcggcga ttttctcgat ttcgtctttt tcaagaaaaa cctgtgggag cgtttcaaga
     3601 catgcccggc cgcagtttct caccatcacg cgtacgtcgg cggtcttctg gagcatacga
     3661 tagcggttgc gcgcacggcg aaaaatatcg tcgaagcggg aatatccggc gggtacgagg
     3721 cggacgttgc cgtcacattg gcgggagcgc tgctccatga tcttggcaag atggaggcgt
     3781 accgccttgt tcctgcgcct gtaatgaccg tggcggggac ctttcacgat catattgtga
     3841 ttgggtacag catgtttgaa tccctcgccc gagaattcaa cctggatgaa cggctttttc
     3901 tggccatagg gcatatcatt gtcagccatc acggatgcag ggagtacggt tctcctgtgc
     3961 ttcccgcgac tccggaagcc atggcggtga gcgcggccga cgagatggat ttcaggctct
     4021 tctgctggaa gcattctgtc gatcagatgg aagatggacg ggaaatatcc gattttaatc
     4081 cttccgccca aagacgtttc tggaaatggg agctgaacaa tggggtataa actgcatatt
     4141 tcaccttctg atgacgggcg tcgcctggac aagacgatcc ggtcgatctg gcctgcgttg
     4201 ccgctcagcg ttgtaatgaa aggcatccgt acaggcgctg tccggctgga cgggaaaaag
     4261 gcggcgtgcg acgtcaggat aacggcgggg caggagctgt atgtcccttg ggaagggcct
     4321 gccgaaaggg aagagggctt ccgctatcgt cgcacacttc ccattctgtt cgaggacgag
     4381 cgcttgctgg ctgtgaataa gccggaaaac ctgcttgtcc agccggaccg gaaggatcag
     4441 gacaatgtgc tcgacagggt gaagtacatg cggagtcttg aaggggccga ggagagagcc
     4501 tacgcagtgc accggctgga caggaacacg acgggagtgc ttctggtggc gctctccggg
     4561 gtctctctcc gcatactcca ggatgccttc cgggctcgtg aagttcgaaa gacgtatctt
     4621 gccgtagttt caggggttcc tcctgcaaag ggagagatcg acgccccttt gctcaaggat
     4681 cccgcttcca acaccgttaa ggtggatcct tcggggaagg aggcgctcac tcgctacaga
     4741 cttcttgcag ccggagggga cgtttctctc gtcgaaatcg acctcgtgac ggggcgctct
     4801 caccaggccc gggtgcacat ggcgtcgata ggtcattcga ttctcggaga tattcgctac
     4861 ggcagcgaaa atgtcaatgc gaagtggagg aagaggggga ttcgccgccc tcttctgcat
     4921 agttatcagg ttgtcttcgg gagccttctg ggagaacttg aatcgctttc aggcatgcgg
     4981 atagctgctc ctcttccgga ggatatgggg gagcttctac gttcgagggg ctggaaacct
     5041 ccggtataac ccggttctac ccgcgaagga caaacttcgt cagggaagga gggaagagca
     5101 tggaacatgt aaaaatcgtt gcggaagaga agattctgga aaagctgcgg aagaggggag
     5161 aagtctatgc aatccggcgg acactcggtg tcggctgaaa tgccaggaga gtcgtccgta
     5221 tagaggacgg gaagcctccg gtggaggcgg agtttcaaaa aattgagcag gatggcgttg
     5281 tcctctgggt tccgtcattc atggagttca agaacaacga aattctcatt gtacagcgcg
     5341 gatttctctg gtctgcatat ctgatgcttg tcggggtgct cagtgaatcg gaagaatcgt
     5401 gctgtaattc gtgagattct gagaacccaa acgctgcaag cctatggtac ttcgtttctc
     5461 cgttaaagag attttgtgtt ttgtggcggg tatttaggat tattttgatg tatttcaatt
     5521 tctcgcttgt tctttggaaa aagagtgcta tgatagttac taaggtctca aacggctcag
     5581 gccgtaaata tacaggaggt gtgtttcatg tctgttgtaa agcgtacttc ctcgaacgtg
     5641 cttctagaca ccgcactcaa aaatttctat gccgcagcgg aggagatggg gcttgaagag
     5701 ggccttatcg atattctcag ccattccgaa cgcaagacca gcgtgtccat ccctgtcgag
     5761 atggacgacg gatctgtgaa ggtcttcgac ggctaccggg tgctccattc ttccgcgata
     5821 ggccccggca agggcggcgt ccgtttccac ccggaagtga acgttgaaga gtgcgaagcg
     5881 cttgccatga tgatgacatg gaagtgttcg ctggccggta tcccttacgg cggcggcaag
     5941 ggcggagttt cctgcgagcc tctggaactc tcgaagaagg aaaaagaacg tatttcccgc
     6001 acatgggctg cccgcatggc tcccgtgatc ggcgcatgga ccgacgttcc cgctcctgac
     6061 gtgaacaccg gcggccctga aatggtctgg tttatggata ccatcagcaa gatgaagaac
     6121 cagcttgaac ccgccatctt cacaggcaaa cccattccgc tgtggggttc caaaggacgc
     6181 accgcggcga ccggttacgg cgttgcaacc tgcgctcttg aactgctgaa gaacctcggc
     6241 aaagacccga aagcaatctc tgtctcgatc cagggcttcg gcaacgtagg aacctacgcg
     6301 gcccttacta tggtgaacgc cggttccaaa gtcgtcgcca tcagcgacat taccggaacc
     6361 tactttgcag agaagggact tgacataaag aaagctatcg accatgtgaa caaccatccc
     6421 aagcacctgc ttgaaggctt cacctgcgac ggctgccaga agctcgacct tcccgaagct
     6481 ctctttgtca actgcgatat tctcattccc gcagctcttg aaggcgtcat caatggaaag
     6541 aatgctgaca aagtcaaagc caagtacatc gttgaggctg caaacggccc cgtgactccg
     6601 gaaggcgacg ctattcttga cgctaagggc gttctcgttg tcccggactt cctcgccaat
     6661 tccggcggcg ttatcggctc ttacttcgag tgggcgcagg atctcggcgg tttcttctgg
     6721 tcggaggagg agtacaacga gcgcctcatc cgcatcatga aggacaactt ccagcgcgtg
     6781 tggggctacg caaaggacaa gaacgtgaag atgcgccgcg cagcgttcat ggcggcgatt
     6841 cagagagtcg ccgatgcagt tcgtatgaga ggcgtcttcc tgtaagttcg agctcaaact
     6901 gcttttctga tacatctgaa aacgaccggg caaccggtcg ttttttttat tgaaaagtgg
     6961 ggcaggaaaa gagttgtgtg ataaaataat acacgaattc caactgttca attaagttgt
     7021 gagcgtccag tagcagttcg tttccagaag attggaggga tgaaaggtga atccgcaggc
     7081 tatgttttta cagcagcttg aacaggcggt tccgtatgtg actgtggagc cggagtacct
     7141 ggaaattctc aggcatccga aggaggtgct tgaactctct ttgcctgtgc gcctcgacgg
     7201 aggaggcgtg aaggtgttca aggcgtggag gagccaccac aacaacgctc ttggccctta
     7261 caagggcggc gtgcgctatc atgcggaggt cagcagggat gaagtggtcg ccctttcatc
     7321 gtggatgacg atcaaatgtg caattgcagg gctcccttac ggaggaggaa agggaggaat
     7381 ctctgtcgat ataagggagc tttcccgcaa cgaacttgaa actctgagca gagcgtatgc
     7441 tttcggactt gcccgattta cggggacgga ttacgacatc ccggcgccgg acgtgtacac
     7501 aaaccctcag atcatgtcgt ggttccttga tacgtatgaa aaagtcaaag gatggagcga
     7561 atcgtcggca tataccggga aacctcctgt tctggggggc tctctgggaa gatcggatgc
     7621 tacggggaag ggaggcgtct ttattctccg tgaggggctt gcaaagctcg gccttctggg
     7681 gaagaaagat ttgaaggttg gaatacaggg ctacgggaac gcggggacgc gtgctcaccg
     7741 ctttgtggaa gatgatcttg gactaacggt tgttgcagta tcagacagcg gggcggcact
     7801 ctataacccg aaagggctcc cgtatagaga agtctctaaa cataagaaca atacccgttc
     7861 ccttaaagga tttcagggag ctgaggagct cttccccgat cttctcttcg gacttgacat
     7921 ggatattttg atcctcgcag cacttggagg cgctgtggat gctcagaatg cgccggacat
     7981 taaagcgcat gtactgctcg aaattgcaaa cggtccggtt acgcccgatg ctgaggccct
     8041 gcttaccgat aaaggcgtta cgattattcc ggatgttctt gcaaatgcgg gaggggttat
     8101 cgtcagttgc ttcgaatgga tacaggggag atcaggagac gtatggacgg aggagtacgt
     8161 ttttcagaag cttgacgaac gccttactct ttcatttaag gacgtcatga aaatcgctga
     8221 ggacagaaag ataagcgcca ggaaggcggc atatgtcaag gcggttggaa gaatcgtcga
     8281 tgcgatgcga ttcaggggga tatggcccta aaaatgaaga agagcggagg ccggattctt
     8341 ttctttgagt cggtctccgc tcttctgtag agagaggttt atgaagatag acgttcctct
     8401 cttacgatca atcgtaaaaa accctctctt gaggcgcctc atctggattt ttaaagtagg
     8461 cccccaggcc gtttcccatt atttcccggt aatccgggat tccttcaagg atgatcgttt
     8521 tcttcggggc gagcat
//
LOCUS       DAOTIL010000014         8481 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminobacteriaceae bacterium isolate AMR_MDS_3026
            NODE_10042_length_8481_cov_3.386898, whole genome shotgun sequence.
ACCESSION   DAOTIL010000014 DAOTIL010000000
VERSION     DAOTIL010000014.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868050
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminobacteriaceae bacterium (wastewater metagenome)
  ORGANISM  Aminobacteriaceae bacterium
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae.
REFERENCE   1  (bases 1 to 8481)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8481)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:19:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,224
            CDSs (total)                      :: 3,198
            Genes (coding)                    :: 3,184
            CDSs (with protein)               :: 3,184
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8481
                     /organism="Aminobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10042_length_8481_cov_3.386898"
                     /isolate="AMR_MDS_3026"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:3034609"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            159..1949
                     /locus_tag="P5201_00590"
     CDS             159..1949
                     /locus_tag="P5201_00590"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_process: GO:0009966 - regulation of signal transduction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HRV97059.1"
                     /translation="MTAEYVGTTLLLEKNLPPDRSLRRRLERCAGFVVEVSSAEEARR
                     LLSEGGVALFVADALFASPRETAEILRSLLAVRPVPLLLVFPSSGEEITDELAVFAQS
                     GFVTQETPESLLRIAIDNARRLFEIRMCPPVKRTIGQCAACGLRERCELRMSEERFRS
                     LVANIPGVVFRCRSDPEWTMLYMSDLVEELTGYPKEDFIENARRSYVTMIHPEDVPSV
                     ARRITEAIGRRLSWEIEYRVLRKDGGERWVYEKGVPVFDEDGALLFLDGFIFDITGRK
                     RVDDQLNSERKRLANVIEGANAGTWECNLQTGETLLNDKLLELLGHAPGDLDPVTMDV
                     WLSLLHPEDLGQVGDILAGHVRGELENYDHEVRMQHRDGHWVWMHCKGRIISRTPDGE
                     PLWMYGTYIDVTDRKQAEKELLEANRRLLAMQKVLKREATRDSLTGVLNRRAILEHLN
                     EELARCRRDGTCLSAAICDIDHFKEVNDTYGHQTGDDVLCGLVRLLRSNLREYDKIGR
                     IGGEEFLLVLPGEAGRSAGFHERLVKSVAEHSIQTRSGAVRITLSLGAITVSGDADTD
                     EILARADKAMYKAKAEGRNRVVFISDAKAP"
     gene            2142..2600
                     /locus_tag="P5201_00595"
     CDS             2142..2600
                     /locus_tag="P5201_00595"
                     /inference="COORDINATES: protein motif:HMM:NF012791.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HRV97060.1"
                     /translation="MKKVVVPIDGSDTSKGVVDYAVYYANREHDANMLFLHVITVMEH
                     EPVFYGEGEVVLPPSDEKVTAEFTKFIEDRMEAAGMRIPRMSVSVRSGKVYEQIVRFA
                     EEQDADMIMIGHRGLSPMQRFFLGSVAAKVVAQAPCSVYVHRPKERREKA"
     gene            2738..4606
                     /locus_tag="P5201_00600"
     CDS             2738..4606
                     /locus_tag="P5201_00600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014163909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde ferredoxin oxidoreductase family
                     protein"
                     /protein_id="HRV97061.1"
                     /translation="MDILAGYAGKVLRVDLSSGAVSTELLTEEKLRPFLGGTGYAARI
                     LYDELKAGTDPLGPDNLFVLATGPLTDNLVPGGGSLSFCCKSPLTGGWAEARVGCDMG
                     GTLKKAGYDFVVLSGRAERPVHLVITDGRAELESAEHLKGRTVLEKNALLNEGGYSEY
                     DSLVIGPGGERLVLFASVMVGHRAAGRVGCGAVLGSKNVLAVSVRGSGKVVPADAAGY
                     MKAVREAHRIVKESPNTYGFTELGTMGGYEACDAMGDLPTKNWQSNSWGKGAQITSHF
                     VEENFTGGTGCYKGCPMKCGRKVRVKDGKWKTPEHDGGEYETIGSFTAYMLNEDVDCA
                     VHCGYLCNELGIDTISAGGAIGFLMECAEKGLLSPEMTQGLDLSWGNASVLPKLVRMI
                     ALREGVGDILADGVRRAAERIGGGSEAFAVHVKGLEGPCHDPRAAKTLGVNYGTGNRG
                     MCHIHPLETVAYDASKFDFGLRPFGLQDPEELPRWEERGKGTAIRILQDAGALPEILG
                     TCKFYMYVGITPREYAEMLSALTGWNVTGEELMETAERATNLQRLFNIREGFSREDDM
                     LPGRVRSAPAFGTYAGNAECAVRDFDGMLDDYYEARGWDRATGAPTRETLVRLGLA"
     gene            4769..5065
                     /locus_tag="P5201_00605"
     CDS             4769..5065
                     /locus_tag="P5201_00605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013049200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HRV97062.1"
                     /translation="MTTENAVTVSGTLHLIKSDPSSPGKQKKYLHFSVRQDTQKTDGS
                     VRKDFLLARVYDPKLQESFKELEEGAKVLVRGDVRSSLGSGEMYINVQEIEKLA"
     gene            5372..6967
                     /locus_tag="P5201_00610"
     CDS             5372..6967
                     /locus_tag="P5201_00610"
                     /inference="COORDINATES: protein motif:HMM:NF014795.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-binding protein"
                     /protein_id="HRV97063.1"
                     /translation="MVGNTGGNIAVRVLERLNISVLFGIPGIHNLAVYDGLAHSSIRH
                     IATRHEQGAGFMAYGWGKSTGDPGTALVITGPGLTNILTPMGQAFHDSVPMVVISTQI
                     PSRFLSARSGYLHELRDSTTMSSSVTKGSFRASSPSEIAPLLDRAYRLASAGRPGPVH
                     VEIPLDYLAEPCAPSGAFSSSEQPFFIPDEEFTEALRALGRAQFPVILAGGGASRSAV
                     AVRRLAEALNAPVLLTCAGKGILSEEHPLCLGARLHFPAVRELLAKADCLLALGTEIS
                     PTDLWTDEFEFSGTVVSVDNDEGHRASRNGVFLHGRCEEVLPLLLAGVAKKGAARWAE
                     KDALLARCSQELGATLGVADELPLLAETTRALRTSLGDDGTLWADMTGPAYYAISEYA
                     AALPGRFLHPVGFGTLGAALPAALATKCAFPERRVAALAGDGGFQFTLPELAVGVQEK
                     LSLPVILWNDGGFGEIRRSQQAKGGPLIAVDHWNPDFRALAASYGIPYFAPEEGALGD
                     ALEEAFAAPVPSLIEIRARKECC"
     gene            6967..>7164
                     /locus_tag="P5201_00615"
     CDS             6967..>7164
                     /locus_tag="P5201_00615"
                     /inference="COORDINATES: protein motif:HMM:TIGR01141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol-phosphate transaminase"
                     /protein_id="HRV97064.1"
                     /translation="MIAGVRKAVGAATPYVPGKTVEEVRRELGLEKIVKLGSNENPYG
                     PFPAARRAMTEEIGRLNTYPDV"
     assembly_gap    7165..7174
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <7175..8088
                     /locus_tag="P5201_00620"
     CDS             <7175..8088
                     /locus_tag="P5201_00620"
                     /inference="COORDINATES: protein motif:HMM:TIGR01141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HRV97065.1"
                     /translation="REIKSLIGERFGLGPEWVAISHGAEGMLQTLGKVFLEPGDEAIL
                     PCATYGLYREISLVMGATLKEIMLKDDLSVNLREMERAITGRTKLVWLNNPNNPTGML
                     LPKKSVETLIKRLPRGVWLVLDEAYAEFASPEELPSVAKLLEAECSLIVVRTFSKAWG
                     LAGARIGYALARPEMVRIIDTVSEPFNANRIAIAGASASLKDDEPAFNQALAATVRER
                     ERVTKALRDMGGETLPSHTNFVFVTFQADAGDISGKLLRKGVIVRPCGGWGFGCSLRV
                     TIGTPEQNDFFLEALRGVLSGDGEGGA"
     gene            8085..>8481
                     /locus_tag="P5201_00625"
     CDS             8085..>8481
                     /locus_tag="P5201_00625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014163919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HRV97066.1"
                     /translation="MSTERIASGDGVVFTDVCKIYPDGTRAVDMLNLDIRRGELVSLI
                     GPSGCGKTTTLKMINRLEEPTSGSIVVGGEDVRSSDAVALRRRIGYVVQEIALFPHLT
                     VAENINVVPELLGWSAAKMRSRADELLEMA"
BASE COUNT         1652 a         2407 c         2831 g         1581 t
ORIGIN      
        1 atacagtgtg ctccgacaat ttatgctatt tcaaggagta aagcgcctaa aaatcccttg
       61 tcttgaagta attcatcctt ctctctcttc caagataaat atgtataaaa gagtgttcaa
      121 ggtgcagtat attcatttga atggtacgag gttgcagaat gactgctgaa tacgtcggta
      181 caacgctcct tctggaaaaa aatcttccgc cggacagatc gctgaggcgt cgccttgaac
      241 ggtgcgccgg tttcgttgtc gaagtctcct ccgccgagga agcccgcagg ctgctctcgg
      301 aggggggtgt tgcgctgttc gttgccgacg cgctctttgc gtctccccgg gaaacagcgg
      361 aaattctgcg ttcgcttctc gccgtccgcc cggtcccgct gctccttgtt tttccctcgt
      421 ccggcgagga aatcacagac gaactcgccg tcttcgcaca gtcgggtttt gtaacgcaag
      481 agacgcccga gtcgcttctt cgcatcgcaa tcgacaacgc ccgccggctc tttgaaatcc
      541 ggatgtgccc ccctgtcaaa cggacgatcg gccagtgcgc cgcctgcggg ctgcgcgaac
      601 ggtgcgagct gcgtatgagc gaggaacgct tcaggtctct tgtggcgaac ataccgggcg
      661 tcgtcttccg ctgccgctcc gatccggaat ggacgatgct ctacatgagc gaccttgtgg
      721 aagagctgac gggctacccg aaggaggatt tcattgaaaa cgcgaggcgc agctatgtga
      781 cgatgattca cccggaagac gtcccatcag tagcgcgacg catcactgaa gcgatcggac
      841 ggcgtctttc ctgggagatc gaataccgcg tgctccgcaa ggatggcggc gaacgctggg
      901 tgtacgagaa gggcgttccc gtgttcgatg aggacggagc gcttctcttt cttgacggtt
      961 ttatcttcga catcaccggc cggaagcgtg tggatgacca gctgaattcg gagaggaagc
     1021 ggcttgccaa tgtcatcgag ggggcgaatg ccgggacctg ggagtgcaac ctgcagaccg
     1081 gcgagacgct ccttaatgac aagttgcttg agcttctggg gcacgccccc ggcgaccttg
     1141 accccgtcac aatggatgtg tggctgagtt tgctgcaccc cgaggacctg gggcaggtcg
     1201 gggatatcct cgccgggcac gtgcggggag aactggagaa ctacgaccac gaggtccgga
     1261 tgcagcacag ggacgggcat tgggtctgga tgcactgcaa ggggcggatc atctcgcgaa
     1321 cgccggacgg cgaacccctg tggatgtacg gcacctatat cgacgtcaca gaccggaaac
     1381 aggcggaaaa ggagcttctg gaggccaacc gcaggcttct ggcgatgcag aaggtgctga
     1441 aacgggaggc gacgcgcgac tccctgacgg gcgtgctcaa tcggagagcg atactggagc
     1501 atctgaatga ggaactggcg cgttgccggc gggacggaac gtgcctgagc gcggcgatat
     1561 gcgatatcga ccacttcaag gaggtcaacg atacgtacgg gcatcagacg ggcgacgacg
     1621 tcctgtgcgg gctggtccgg ctgctgcgga gcaatctcag ggagtacgac aagatcggaa
     1681 gaataggcgg ggaggaattc ctgctggttc tccccggaga ggcggggcgg agcgcggggt
     1741 ttcatgaacg gctggtgaaa agcgttgcgg aacactccat ccagacccgt tcgggagccg
     1801 tgcgcattac gctgagcctc ggagcgatca ccgtttccgg cgacgccgac acggacgaaa
     1861 ttcttgcgcg cgcagacaag gcgatgtaca aagccaaggc ggaggggcgc aaccgggtgg
     1921 tcttcatcag cgacgcgaag gctccctgag caggcgcttt ccgggagcaa gatgtatcgg
     1981 ccgttcggga ggacgctgga gagctttttg agcaggagag ggaaaaattg tcgtaaaatt
     2041 gactaaatag actggggaat gctatacttc actcaagacc ttccggcgct gacgggactg
     2101 gagttcggat gcccgaaacg ggcgtaaagg aggggttgcg gatgaaaaaa gttgtggtac
     2161 ccatcgacgg cagcgatacc agcaaagggg tcgtggacta cgccgtatac tacgcgaaca
     2221 gagagcatga cgcgaacatg ctcttccttc atgtgatcac cgtcatggaa cacgagcctg
     2281 tcttctacgg cgagggggag gtggtgcttc ccccgtccga cgagaaggta acggcggaat
     2341 tcacgaagtt catcgaagac cggatggaag cggcgggaat gaggattccg agaatgtccg
     2401 tttcagtgcg ctcgggcaag gtctatgagc agatcgtccg ttttgccgag gagcaggatg
     2461 ccgatatgat catgatcggc cacagggggt tgagtccgat gcagcgcttc ttcctcggga
     2521 gcgtggccgc gaaggtggtg gcgcaggcgc cgtgcagcgt ctacgtccac cggccgaagg
     2581 agcggcggga gaaagcatga agcccgaacg gcgggccgga agggatgact gttctttcga
     2641 cggacagtgt acaaaaacga cgggcggggg tgtctctccg cccgtcgtgt ggtatggtag
     2701 gaggatggag aaacgagaga agaagggaga cgattgaatg gatatacttg ccggatatgc
     2761 cggaaaggtg ctgcgcgtcg acctctcctc cggagctgtt tcgacggagc tgctgacgga
     2821 ggagaagctg cgcccgttcc tgggagggac ggggtacgcc gcccgcattc tgtacgatga
     2881 actgaaggcc ggaaccgatc ctctcgggcc tgacaatctc tttgtcctcg cgacggggcc
     2941 gctgacggac aacctcgtcc cgggaggggg aagcctttcc ttctgctgca agtcgccgtt
     3001 gaccggaggg tgggccgagg ccagggtcgg gtgcgacatg ggaggaacgc tgaagaaagc
     3061 gggctacgac ttcgtcgttc tctccggaag agcggaacgc cccgtccacc tcgtcatcac
     3121 ggacggtcgg gcagagctcg aaagcgcgga gcacctgaaa ggcaggacgg tgctggagaa
     3181 aaatgcgctg ctgaacgagg gcggctattc ggagtacgat tcgctcgtca tcggcccggg
     3241 cggcgaacgc cttgtactgt tcgcctccgt tatggtcggg catcgggctg cgggccgggt
     3301 cgggtgcggg gccgtcctcg gctcgaaaaa cgtgctcgcc gtttcggtca gagggagcgg
     3361 gaaggtggtt cccgcggacg cggccggata tatgaaggcc gtccgcgagg cgcaccgcat
     3421 cgtgaaggag agccccaaca cctacggttt caccgagctc ggaacgatgg gcggctacga
     3481 ggcctgcgac gcgatgggcg acctcccgac gaagaactgg cagtcgaact cctgggggaa
     3541 gggtgcgcag atcacctcgc acttcgtcga ggagaacttc accggcggaa cagggtgcta
     3601 caaggggtgc ccgatgaagt gcggcagaaa ggtgcgcgtg aaggacggaa agtggaagac
     3661 gccggagcac gacggcggcg agtacgagac gataggctcg ttcaccgcgt atatgctcaa
     3721 cgaggatgtc gactgtgccg tccactgcgg ctacctctgc aacgagctgg gaatcgacac
     3781 gatctccgcg ggaggggcca tcggcttcct gatggagtgc gccgagaagg ggctcctctc
     3841 gcctgagatg acgcaggggc tcgacctctc ctgggggaac gcctccgtgc tcccgaagct
     3901 tgtgcggatg atcgcgctcc gggagggtgt gggggatatc ctcgccgacg gcgtccgccg
     3961 ggctgcagaa cgcatcggag gcgggtcgga ggcgttcgcc gtccacgtca aggggcttga
     4021 aggtccgtgc cacgacccga gggcggcgaa aacgctcggc gtgaactacg ggaccggcaa
     4081 ccgcgggatg tgccatatcc acccccttga aacagtggcc tacgacgcgt cgaagttcga
     4141 tttcggcctg cgtcccttcg gcctccagga cccggaggag ttgccccgct gggaggagcg
     4201 gggaaaggga acggcgattc ggatcctcca ggatgcaggg gctctgccgg agattctggg
     4261 gacctgcaag ttctacatgt acgtcggcat caccccgcgg gagtacgcgg agatgctttc
     4321 ggcgcttacc ggatggaacg tcacgggaga ggaacttatg gagacggcgg aacgcgcgac
     4381 gaatctccag cgccttttca acatccggga gggcttttcc agggaggacg acatgctccc
     4441 cggaagggtg cggtcggcgc ccgcgttcgg gacgtacgcg ggaaacgcgg aatgcgcggt
     4501 gcgcgacttc gacggcatgc tcgacgacta ctacgaagcc cgggggtggg acagggcgac
     4561 cggcgcgccg acgcgggaga cgcttgtccg tctcggtctg gcgtagcagg atactgcgcg
     4621 cgctccttga aatatagggt acatcgtata tttcgtttcg gaaacaaggc gacaaagagg
     4681 ggcaaggatg gtacaatatg gtaacaattt gaaggacggg gcgctcgcaa gagaccccgt
     4741 acattccgag aacgggagtt gatgtccgat gacgacggaa aatgccgtga cggtttcagg
     4801 gacgctccat ctgatcaaaa gcgatccgtc cagccccggc aagcagaaga aatatcttca
     4861 tttctcagtg cgtcaggata cgcaaaagac ggatggaagc gtccgaaagg attttcttct
     4921 cgccagagtc tatgacccga agcttcagga atccttcaag gagcttgagg agggcgcgaa
     4981 agtgctggtt cgcggagatg tgcgttcctc tctcggcagc ggcgagatgt atatcaacgt
     5041 ccaggagata gaaaagctgg cgtagagttt ttcgtatgaa ccgaggaacg tcttctcctg
     5101 acgtttctca tgcgtttcct tcattacagg gcttggtgtc gatttttcga ctgtcgatac
     5161 cctttgtcgg ttgtgtgccg aaaaaagtgg cgtttctccc attcatcgca cggagtcttc
     5221 cgcgctatgt tgtttttttc cccaagcccg accactctgc cgccatgcgt actgcataga
     5281 agttgtacgg ggtgtactct tgcgcacagc gcaaggggaa cccctttcag atgttttccc
     5341 cttaaaatcg gacattcagg aggtcgattc aatggtaggg aataccggcg ggaatattgc
     5401 cgtccgggtc ctggagcgtt tgaacatttc agttctcttc ggcataccgg gcattcataa
     5461 cctggccgtc tacgacggtc ttgcgcacag ctccatccgg catatcgcaa cgcggcacga
     5521 acaaggggcc gggttcatgg cgtacggatg gggaaagtcc accggcgacc ccggaacggc
     5581 gctcgtcatc acggggccgg ggctcaccaa catcctcacc cccatggggc aggcgttcca
     5641 cgattcggtt ccgatggtcg tcatctccac gcagatcccc tcgcgttttc tctcagcccg
     5701 ttcaggatat cttcacgagt tgcgtgattc cacgacaatg tcctcttccg tgacgaaagg
     5761 cagctttcgc gcctcctctc cctcggagat cgcgccgctc cttgatcgcg cgtaccggtt
     5821 ggcctccgcc ggccggccgg ggcccgtcca cgtggagatt ccgctcgact acctcgccga
     5881 accgtgcgcg ccttcggggg cgttttcttc gagcgaacag ccgtttttca tccccgacga
     5941 ggagtttacc gaggctcttc gggcgctcgg gcgagcccaa ttccccgtga tcctcgcggg
     6001 gggcggggcg tcccgctccg ccgttgcggt ccgcagactg gccgaggcgc tgaacgcacc
     6061 ggttctgctc acgtgcgcgg ggaagggcat tctctccgaa gagcatccgc tctgcctcgg
     6121 cgcgcgcctt cactttcccg cggtcaggga gctgcttgcg aaagccgact gcctcctcgc
     6181 gctcggtacc gagatctccc cgaccgacct ctggacggat gagttcgagt tctccggaac
     6241 tgtggtctct gtggataatg acgaggggca tcgcgcctcc cgcaacggag tgttcctgca
     6301 cggaaggtgt gaggaggtgc ttccgctgct gctggccgga gtcgccaaaa agggcgccgc
     6361 gcggtgggcg gagaaggacg cgcttcttgc ccgctgctcg caggagctgg gcgcaacgct
     6421 tggcgtcgcg gacgagttgc cgctgctcgc agagacgacg agggcgctca ggacctcgct
     6481 cggcgacgac ggaacgctct gggcggatat gacgggcccg gcgtactacg cgatcagcga
     6541 gtacgccgcc gcgctccccg ggcgtttcct ccacccggtc ggcttcggga cgctcggcgc
     6601 ggcgttgccc gccgcgttgg cgacgaagtg cgcctttccg gaacgccgcg tcgccgcgct
     6661 cgcaggcgac ggaggttttc agttcacgct tccggagctc gcggtgggtg tccaggaaaa
     6721 actgtcgctc ccggtgatct tgtggaacga cggcggcttc ggcgagatcc ggcggtccca
     6781 gcaggcgaaa ggcggtccgc tcatcgccgt cgatcactgg aacccagact tccgtgcgct
     6841 ggcggcctcc tatggaatcc cctatttcgc gcctgaagaa ggcgcgctcg gcgacgcgct
     6901 cgaagaggcg ttcgcagccc ccgtcccgtc gttgatcgaa atccgcgccc gaaaggagtg
     6961 ttgctgatga ttgcaggagt cagaaaagcc gttggagcgg cgaccccgta tgttccgggg
     7021 aagaccgtcg aagaggtccg cagagagctg gggctcgaaa agatcgtgaa gcttggctcg
     7081 aacgagaacc cgtacggccc cttccccgca gcccgcaggg caatgacgga ggagataggg
     7141 cggctcaaca cctacccgga cgtcnnnnnn nnnntccgcg aaatcaagag tctcatcggc
     7201 gagcgcttcg ggctcggccc ggagtgggtg gcgatctcgc acggcgccga agggatgctc
     7261 cagacgctcg gcaaagtctt tctggagccc ggcgacgagg ctattcttcc gtgcgccact
     7321 tacggactct accgggagat ctcgctcgtt atgggggcga cgctgaaaga gatcatgctc
     7381 aaggacgatc tctccgtcaa cctgcgggag atggaacgcg cgatcaccgg gcgcaccaag
     7441 ctggtgtggc tgaacaaccc gaacaacccc accgggatgc tgctccccaa aaagagcgtc
     7501 gagacgctga taaagcgcct tccccgcggg gtctggctcg tcctcgacga agcgtacgcc
     7561 gagtttgcct ccccggaaga gctgccttcg gtcgcgaagc tgcttgaggc agagtgcagc
     7621 ctgatcgtcg tgcggacttt ttcaaaggcg tggggactcg ccggggcgcg tatcgggtat
     7681 gcgctggcgc gtccggagat ggtgcggatc atcgacacgg tgagcgagcc gttcaacgcc
     7741 aacaggatcg ccatcgccgg cgcctcggcg tcgctgaagg atgacgagcc cgcgttcaac
     7801 caggcgctcg cggcgacggt gcgggagcgg gagcgggtaa ccaaggcgct tcgcgatatg
     7861 ggaggcgaaa cgcttccttc gcacaccaac tttgtcttcg tcaccttcca ggcggatgcg
     7921 ggcgatatct cggggaagct gctccgcaag ggcgtcatcg tgcgcccctg cggcggctgg
     7981 gggttcggtt gttcgctccg cgtgacgatc ggcacgccgg aacagaacga cttcttcctt
     8041 gaggcgcttc gcggcgttct ttcgggagac ggggagggag gagcatgagc acggagcgaa
     8101 tagcttcagg agacggcgtc gtcttcacgg atgtctgcaa gatctaccct gacggaacga
     8161 gggcggtcga catgctgaac ctcgacattc gcagggggga gctggtcagc ctcatcggtc
     8221 cgtcggggtg cggcaagacg acgacgctca agatgatcaa ccgcctcgaa gaaccgacgt
     8281 ccgggagcat cgtcgtcggc ggggaggatg tccggtcgag cgatgctgtg gcgctccgtc
     8341 gccgtatcgg gtacgtggtc caggagatcg cgctctttcc gcacctcacc gtggcggaga
     8401 atatcaacgt cgttcccgag ctgctcggat ggtctgcggc gaaaatgcgg agcagggcgg
     8461 acgaactgct tgagatggcc g
//
LOCUS       DAOTIL010000015         8436 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminobacteriaceae bacterium isolate AMR_MDS_3026
            NODE_10117_length_8436_cov_4.023625, whole genome shotgun sequence.
ACCESSION   DAOTIL010000015 DAOTIL010000000
VERSION     DAOTIL010000015.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868050
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminobacteriaceae bacterium (wastewater metagenome)
  ORGANISM  Aminobacteriaceae bacterium
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae.
REFERENCE   1  (bases 1 to 8436)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8436)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:19:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,224
            CDSs (total)                      :: 3,198
            Genes (coding)                    :: 3,184
            CDSs (with protein)               :: 3,184
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8436
                     /organism="Aminobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10117_length_8436_cov_4.023625"
                     /isolate="AMR_MDS_3026"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:3034609"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..908
                     /locus_tag="P5201_00630"
     CDS             <1..908
                     /locus_tag="P5201_00630"
                     /inference="COORDINATES: protein motif:HMM:NF025953.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DapH/DapD/GlmU-related protein"
                     /protein_id="HRV97067.1"
                     /translation="KDASEAQKALTEVNSGIYIFKTSSLLPHIYRLDNENAQGEYYLP
                     DIIAMMIRAGGKVTASLSADEGEFAGINTPLQLADAATALRRRILRRHLLAGVRMQDP
                     DSVWIGPEVVIAGNVLLEPSVQLWGKTEVESGAHVGSFSILRNVVLEEDACVVSHAVL
                     SDSVFRKGSQVGPFIVVRDGAVFEEDSRGGKFVEVKKSRVGKGSKVPHLAYIGDAEIG
                     AETNIGAGTITCNYDGKKKNPTTIGNRCFIGSDTMLVAPVNVGDDGFTAAGSVITQDV
                     PEGALAVGRAKQRNIEGWAKRAGKK"
     gene            935..1903
                     /locus_tag="P5201_00635"
     CDS             935..1903
                     /locus_tag="P5201_00635"
                     /EC_number="2.7.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013048145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004749 - ribose phosphate diphosphokinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0006015 - 5-phosphoribose 1-diphosphate
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0009156 - ribonucleoside monophosphate
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0009165 - nucleotide biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate pyrophosphokinase"
                     /protein_id="HRV97068.1"
                     /translation="MGTIGRELKVFSGTAHPAFARRVCSELGIELSRAKHFRFSDGEI
                     GLSIEESVRGADVFVVQPTCNPVNENLVELLIMIDALKRASAYRINVVIPYFGYARQD
                     RKTKPRDPISAKLMANLLTEAGATRVISADLHAGQIQGFFDIPVDNLTGIPLLASYFK
                     EILAECVECGSLTVVAPDVGGVVRARKFAGTLKADLAIVDKRRSHEVANLCEVMDVIG
                     DIRGKTAILVDDIIDTAGTICNAAGLLKERGAVKIYACSTHGVLSGPALDRLEHSDID
                     EVVLTDTIPLPESKKSAKIVQLSIAPLFAEAILRVHSDRSVSSLFD"
     gene            1971..2624
                     /locus_tag="P5201_00640"
     CDS             1971..2624
                     /locus_tag="P5201_00640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013048146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0008097 - 5S rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0006950 - response to stress [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L25"
                     /protein_id="HRV97069.1"
                     /translation="MSEMISIVLEERKEIGKEVCKKMRPAGYTPCVFYGPEYVESVPA
                     KVKTAGIAKLLKSGQWETVTFDATLPDGRKEMCLIREVQKDFLNGNVLHIDFMQIIKG
                     HKISVNVPLHITGRDACKGVKLGGVIDQILREISMEVLPGQIPDSFTVDISSLGLGEQ
                     IHVRDLALPSDATLLVDKDDVAVAIIIPRGVAEAEAEEEQKEVEVVAKGKAKSADEE"
     gene            2631..3197
                     /gene="pth"
                     /locus_tag="P5201_00645"
     CDS             2631..3197
                     /gene="pth"
                     /locus_tag="P5201_00645"
                     /EC_number="3.1.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014806560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004045 - aminoacyl-tRNA hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA hydrolase"
                     /protein_id="HRV97070.1"
                     /translation="MKIVAGLGNPGPEYVFSRHNAGWLAVDHLVNRFSCGTPRMQFSS
                     MAWSFFVNGEKILILKPLTYMNLSGKAVGEAVDYLSADWEDVLVVYDDVALPFGKLRM
                     RSKGSAGGHKGLLSILGRAGTLEVPRLRIGVGAAPDARGIVSWVLGNFDKTEQNELPS
                     LLDSTAEAVEEWLSVGTEKAMNRVNATH"
     gene            3274..6414
                     /locus_tag="P5201_00650"
     CDS             3274..6414
                     /locus_tag="P5201_00650"
                     /inference="COORDINATES: protein motif:HMM:NF012492.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helicase-related protein"
                     /protein_id="HRV97071.1"
                     /translation="MKTPVETRKNRVWDVEEALWKQGASLHLPVDGAARCWVCRAPQG
                     PVVAVFPDAKQAGTFLADWKALFPEEPVGYLQEIPLTPQGISNKALAVQRGETLSRWR
                     EEGGVLVATGGGLLGPVARGGGEILLEVEKEYERNALLDWLVHSGYVRSDLVWAPGQF
                     VLRGYILDVYDPAYAYPLRLEFYDDTLESIRSFSPRTQKSVAMLDALHIHSLSITREA
                     SVLDFFTEARPGASTESAGEPFFLLFEPVKIESSAETYELLWKEVASASGAQPLPSWQ
                     TLHLRLASWPTLRISSGVAYARDTLVLEEAPLFKGNLERFQWMCDAWRRDGYHIRLFT
                     ANPRLSSLYDEDLSVIEGALSKGFVDPLKRIVHISDLELSGISDRVASAGTVFAPPRE
                     WSEQLVEGRLLIHEEYGLCVFRGSEEVAISGEVMDSLVLEFADNKRLFLPVLQLHKIT
                     PLSEHADDDVQLDSLRGTRWKKSVAKTKERVLQEVRALLELYAKRELVEGFAFPPRDD
                     LFDQFERSFPHAETKDQLVAISEILEDMERRFPMDRLLVGDVGYGKTEVALRAAFRAV
                     QGGKQAALLVPTTILAQQHYLSFTARLTGFPVRVAFLSRFLSRKEQKDVLERVAAGEV
                     DILIGTVRMLREDVAFKDLGLLIIDEEHRFGVMSKEKLKQARENVDVLMLSATPIPRT
                     LALSLKGLRSISVLNEAPHNRIPVVTTAGPWSASIVKGAVSRELERGGQVYYVTNRIT
                     RMPKRLAFLRRLFPDVAIGMAHGRMKEAELEETMMGFYNGKIQILLCTTIIESGLDIP
                     GANTIVIEDCQELGLAQMYQLRGRVGRREEAAYAYFLYPEGRPLAAETLERLDAITSL
                     NDEGAGYDLALQDLRIRGSGDLLGTSQHGSGRGIADSHLYCTLLEEEIGKIRGVVPSA
                     ATVSAEIPCLVPSFYIPQESIRIALYRRLLRVGSLDELHDLEKEVKDRFGTLPESLEN
                     LFSISFLRSRGGFYGILSMECTRRETTLTGEGPLFDLLRTWKRWSGKEKKLSGPGGAS
                     GLRDVLLGLRQIKQVL"
     gene            6418..7218
                     /gene="mazG"
                     /locus_tag="P5201_00655"
     CDS             6418..7218
                     /gene="mazG"
                     /locus_tag="P5201_00655"
                     /EC_number="3.6.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014806562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside triphosphate pyrophosphohydrolase"
                     /protein_id="HRV97072.1"
                     /translation="METRSGHVFEELLAVMARLRGENGCPWDREQTMETLRTYIVEEA
                     YELVDAISRGETPDIVEEAGDLLLQVVFLSRIAEDEGRFTIDDVLRGLTEKLVRRHPH
                     VFGDEKADTSGQVLRKWEEIKGVEKEQRKKKDTSLLAGVPEGLPPLAKAARIQSKAAH
                     VGFDWEKGNLAPLYDKVDEEIEEIRNAVETGSADRIEDEMGDLLFAAVNLARHLEVNP
                     DSALGRANRKFSDRFRIVESMVRETGRPWSSFSLDTLDTFWEQAKKSL"
     gene            7350..>8436
                     /locus_tag="P5201_00660"
     CDS             7350..>8436
                     /locus_tag="P5201_00660"
                     /EC_number="6.4.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009166182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate carboxylase subunit B"
                     /protein_id="HRV97073.1"
                     /translation="METKSEPLPKKESRQIRKVGITDTTLRDAHQSLMATRMKVEDMA
                     EIIERMDDIGFHSMEVWGGATYDSCMRFLDEDPWERLRTMRRLFKKTKLQMLLRGQNV
                     VGYRHYADDTVREFVKRAVGNGLDIIRVFDALNDLRNMEVAADQIKKEGAHLQLAFSF
                     TLSPVHSLDAFGKLAKDMKSMGADSICIKDMAGIVSPVETAALVKTIKKETGLPVQIH
                     SHYTSGMASMAYYAGLEAGADVVDCAISPFAMGTSQPPVESMVAALKGGDFDTDIELE
                     KLVPIAMHFKKVRDKYSKIFVDLGGVDINVLIYQIPGGMYSNLVSQLKDQNALARLPE
                     VLKEVPRVREEMGYPPLVTPTSQIVGTQ"
BASE COUNT         1825 a         2385 c         2588 g         1638 t
ORIGIN      
        1 acaaggacgc ctcggaggcg caaaaagcgc tcacagaggt caacagcggt atctatatct
       61 tcaaaacatc ctcccttcta ccccatatct accgactgga caacgagaac gcccaaggag
      121 aatactattt acccgacatc atcgcgatga tgatccgcgc gggcggcaag gtgaccgcct
      181 cgctctccgc ggacgagggg gaatttgccg ggatcaacac cccgttgcag cttgcggatg
      241 cggctacggc cctgcgcagg cgtattctcc gccgtcatct cctggccggg gttcgtatgc
      301 aggatcccga ttccgtgtgg atagggcccg aggtcgtaat cgccggcaac gtgctgcttg
      361 aaccgtctgt ccagctgtgg gggaagacgg aggtggaaag tggcgcacat gtcggcagct
      421 tttccattct ccggaacgtc gtcctggaag aggacgcctg cgtcgtgagt catgcggtgc
      481 tctcggacag cgttttccgg aaaggctctc aggtcggtcc gtttatcgtg gttcgcgacg
      541 gagcggtctt cgaagaggac tcgcggggcg gaaagttcgt cgaggtcaag aagagccgcg
      601 tgggcaaagg gagcaaagtc ccccacctgg cgtacatcgg cgatgcggag atcggcgcgg
      661 agacgaatat cggcgccggg acaatcacgt gcaactacga cggaaaaaag aagaatccca
      721 cgaccatcgg caaccgctgt ttcatcggaa gcgatacgat gcttgtggcg cctgtcaacg
      781 tcggcgacga cggttttacc gccgcgggtt cggtaattac gcaggacgtt cccgaagggg
      841 cgcttgcggt gggcagggcg aaacagagaa acatcgaagg ctgggcgaaa cgagcaggaa
      901 aaaagtgaaa tgattgaagg aggcatccgt gatgatggga accatcgggc gtgagttgaa
      961 ggtcttttcc ggaacagccc atcccgcctt cgcacgaagg gtatgcagcg agcttggaat
     1021 tgaactgtcc agggcgaagc acttccgctt ttcggacgga gagatagggc tctccattga
     1081 ggagagcgtt cgtggcgctg atgtgttcgt ggtgcagccg acgtgcaatc ctgtcaacga
     1141 gaatctcgtc gaattgctga ttatgatcga cgcgctcaag cgggcttcgg cgtaccggat
     1201 caacgtcgtg atcccctact tcggctacgc gcggcaggac aggaagacaa agcccaggga
     1261 tcccatctcc gcgaagctca tggcgaacct gcttaccgag gcgggggcca cgcgtgttat
     1321 ttcggcggat cttcacgccg ggcagataca gggatttttc gatatccctg tggataacct
     1381 gacggggatt cccctgctcg cgagctactt caaggagatc ctggcggagt gcgtcgaatg
     1441 cggttccctc acagtagtgg cgccggacgt gggcggcgtg gtccgtgcaa ggaagttcgc
     1501 cggaacgctg aaggcggatc tcgccatcgt cgacaagagg cgttcgcacg aggtcgcgaa
     1561 cctgtgcgaa gttatggacg tcatcggcga tatccgcggg aagacggcga tcctggtcga
     1621 cgacatcatc gacactgcgg ggaccatctg caatgccgcg gggctgctca aggagcgggg
     1681 cgccgtgaag atctacgcct gctcgacgca cggcgttctt tcggggccgg ctctcgaccg
     1741 tctggagcac tccgatatcg acgaagttgt gttgacggat acaatcccat tgcctgagag
     1801 caaaaaatca gctaaaattg ttcagttgtc aatcgcaccc ctatttgccg aggctattct
     1861 cagagtacac agcgaccgct cggtgagcag tcttttcgat taactgcggg gagatgtcaa
     1921 gttttccgat tccgtagaag gagtcgattt tcaaggagga tgtgttgggc atgtcggaaa
     1981 tgatcagtat cgtactggaa gagcgcaagg aaattggaaa agaagtctgc aagaaaatgc
     2041 gccccgcggg gtacactccc tgcgtattct acgggccgga gtatgtggaa tccgttcccg
     2101 cgaaggtgaa aacagccgga atcgccaaac tgctgaagtc cgggcagtgg gagacggtga
     2161 ctttcgacgc gaccctgccg gacggcagga aggagatgtg ccttatacgg gaggtccaga
     2221 aggacttcct gaacggcaac gttttgcaca tcgactttat gcagatcatc aaggggcaca
     2281 aaatcagcgt caacgttcct ctccatatca cggggcgcga cgcatgcaag ggcgtcaaac
     2341 tcggcggggt tatcgatcaa atccttcgtg aaatctccat ggaagttctc cccggccaga
     2401 ttcccgattc cttcaccgtg gacatctcct ccctcgggct cggcgaacag atccatgtgc
     2461 gcgaccttgc acttccctcc gacgctacgc tgcttgtcga caaggatgat gtggcggttg
     2521 ccatcattat tcctcgcggc gtcgccgagg ccgaagcgga agaagagcag aaggaagtcg
     2581 aagtcgtcgc caagggcaag gcgaagagcg cagacgagga ataacgccgg ttgaaaatcg
     2641 ttgctggttt gggaaatccc gggccggagt acgtcttctc ccgtcataac gcggggtggc
     2701 tcgcggtgga ccatctggtg aaccgttttt cctgcggaac gccgaggatg cagttctctt
     2761 cgatggcatg gtcgtttttt gtgaacgggg agaagatcct gattctcaaa ccgctcacct
     2821 acatgaacct cagcgggaaa gcggtcggag aggctgtgga ctacctttca gccgattggg
     2881 aggacgtcct tgtcgtgtat gacgacgtcg cgctcccctt cgggaagctc cggatgcgct
     2941 ccaagggaag cgctggagga cacaaaggac tgctctccat tctcggccgg gcgggaacgc
     3001 tcgaagtgcc caggctgcgc atcggagtcg gcgccgcgcc ggatgcccgc gggattgtct
     3061 cctgggtgct cggcaacttc gataaaacag aacagaacga acttccctct cttctcgaca
     3121 gcactgcgga ggccgttgaa gaatggctct ccgttggaac cgagaaggcg atgaaccggg
     3181 tgaacgcaac tcattgacaa agaggtggcg gagaagtccg ccacctcttt gcgcacagtc
     3241 tcccggagcg gagcggactc cggagtcgaa tgtatgaaaa cccctgtaga aacaagaaaa
     3301 aaccgagtat gggacgttga ggaagcgctg tggaagcaag gcgcctccct tcatcttccc
     3361 gtcgacggcg ccgccagatg ctgggtctgt cgggcgcctc aagggcctgt tgtcgctgtg
     3421 tttccggatg cgaaacaggc cggaacattt ctggccgact ggaaagccct ctttcccgaa
     3481 gagcctgtcg gatatctcca ggaaatcccc ctcacacccc agggaatctc gaacaaggcg
     3541 ctcgccgtac aaagaggtga gacgctctcc cgctggagag aggagggggg cgtgctcgtc
     3601 gctacaggcg gaggacttct tggccccgtc gcgcggggcg gcggcgagat tcttctcgaa
     3661 gtggaaaaag agtacgaacg gaacgccctt ctcgactggc tggtgcattc cgggtatgtc
     3721 cggtccgacc tcgtctgggc gcccggacaa ttcgtactcc gcgggtatat cctcgacgtc
     3781 tacgaccctg cctacgcata tccgctgcgg cttgagttct acgacgatac cctggagagc
     3841 atccgcagct tctcgccgcg aacacaaaag agcgtcgcca tgctcgacgc tctccatatt
     3901 cattcgctct ctatcacgcg cgaagcatcc gtgcttgatt ttttcacgga agctcgccct
     3961 ggcgcgtcga ccgaatccgc cggcgagccg ttctttctcc tcttcgagcc ggtaaaaatc
     4021 gaatcgtccg cagagaccta cgaactccta tggaaagagg tagcttccgc atccggagcg
     4081 cagcctcttc cctcctggca gacgctccat ctccggctgg cttcctggcc tacgctccgc
     4141 atctcttccg gcgtggccta cgcccgcgat acgcttgtac tcgaagaagc ccctctcttc
     4201 aaaggaaatc tcgaacggtt tcaatggatg tgcgacgcgt ggcggcgcga cggataccat
     4261 attcgtctgt tcaccgcgaa cccgcggctc tcctccctct acgacgagga tctctccgtc
     4321 atcgaaggcg cgctctccaa gggctttgtc gatcctctca aacgtattgt ccacatctcc
     4381 gacctcgaac tctccggaat ctccgacaga gtagcctccg ccggaacggt tttcgcgccg
     4441 ccgcgggaat ggagcgagca gctcgtcgaa ggacgccttc tcatccacga ggagtacggc
     4501 ctctgtgttt tcaggggatc cgaggaggtc gcaatctccg gagaggtcat ggacagtctt
     4561 gtgctggagt ttgccgacaa caagcgcctc ttccttccgg tgctgcaact ccacaagatc
     4621 acgccgcttt ccgagcatgc cgacgacgac gtccagctcg attcgctccg gggaaccagg
     4681 tggaagaaga gcgtcgccaa aacgaaagag cgtgttctgc aggaggtgcg ggcgctgctc
     4741 gaactgtacg cgaagcggga gctcgtggag ggattcgcgt ttcctccgcg ggacgatctt
     4801 ttcgaccagt ttgaacgctc ctttccccat gcggagacaa aagatcagct cgtggccatc
     4861 tccgaaattc tggaggatat ggagcggcgc tttcccatgg acagactgct cgtcggcgac
     4921 gtcggatacg gaaagaccga ggttgccctg cgggcggctt tccgcgcagt ccagggcggg
     4981 aagcaggcgg cgctcctcgt gcccaccacc atactggcgc agcagcacta tctctcgttt
     5041 actgcccgct tgaccggttt tcctgtccgg gttgcctttc tctcgcgttt tctctcgcgc
     5101 aaggagcaga aggacgtgct cgagcgcgtc gccgcgggcg aggtggacat cctcataggg
     5161 acggtgcgta tgctcaggga ggatgtcgcg ttcaaagacc tcgggctgct catcatcgac
     5221 gaagagcatc gtttcggcgt catgtccaag gaaaagctca aacaggccag ggagaacgtc
     5281 gacgtgctca tgctctccgc cacgcccatt ccccggacgc tcgcgctctc gctcaagggc
     5341 ctcaggagca tctccgtgct caacgaggcc ccgcacaacc ggattcccgt agtcaccacc
     5401 gccggtccat ggagcgcctc gatcgtgaag ggagcggttt ccagggagct cgaaagggga
     5461 ggacaggtct actacgtcac caaccggatt acgcggatgc cgaaacgact ggcattcctg
     5521 cgtcgtctct ttccggacgt tgccatcggc atggcgcacg gcagaatgaa agaggcggaa
     5581 cttgaagaga ccatgatggg tttttacaac gggaaaattc agattctcct gtgtacgacg
     5641 attatcgaga gcggactcga tatccccggc gcgaacacga tcgtcatcga agactgccag
     5701 gagctcgggc ttgcgcagat gtaccagctc agggggcgcg tcggccgcag agaagaggcc
     5761 gcatacgcct actttctcta cccggaggga cggccgcttg ccgctgagac gctggaacgc
     5821 ctcgacgcga ttacgtcgct caacgacgag ggggccgggt acgatttggc gctgcaggat
     5881 ctgcgcatcc gcggcagcgg cgatctgctg ggaacatccc agcacggaag cgggcgcgga
     5941 attgccgatt cgcatctgta ctgcactctt cttgaagagg agatcggcaa gatcagggga
     6001 gtcgtcccct ccgccgcgac ggtgagcgcg gaaataccct gccttgtgcc ttcgttttat
     6061 attccccagg agagcatcag gatcgcgctc tacaggaggt tgttgcgcgt cgggagcctg
     6121 gatgagctgc acgacctcga aaaagaggtc aaagatcgtt tcggaacgct tcccgaaagt
     6181 cttgaaaatc ttttttccat atctttttta cgttcacggg gcggttttta tggtatcttg
     6241 tctatggaat gtacacgaag ggaaacgacg ctgaccggcg aaggccccct ctttgatctg
     6301 ctccggacgt ggaaacgctg gtccggcaag gagaaaaaac tctccggtcc cggaggggca
     6361 agcgggttgc gtgacgttct cctcggactt cgacaaataa agcaggtgct gtagaatatg
     6421 gagactcgtt ccggacatgt ttttgaagag cttctggctg tcatggcgcg ccttcgggga
     6481 gagaacggct gcccctggga cagggagcag acgatggaga ccctcagaac gtacatcgtc
     6541 gaggaagcgt acgagcttgt agacgcgatt tcgcggggag agacgccgga tatcgtcgag
     6601 gaggcgggcg atcttttgct gcaggtcgtt tttctctccc ggattgcgga agatgaaggg
     6661 cgttttacca tcgacgacgt tctgcggggg ctgacggaaa aactggtccg acgtcacccc
     6721 catgtcttcg gcgacgaaaa agccgatacc agcgggcagg tgctccggaa gtgggaagag
     6781 atcaagggcg tggagaagga gcaacggaaa aaaaaggaca cgtcgcttct cgcgggagtt
     6841 ccggaaggat tgccgccgct ggcgaaggcg gcgcgtattc agtcgaaggc cgcgcatgtc
     6901 ggttttgact gggaaaaagg gaatcttgct ccattgtatg ataaagtaga tgaggaaata
     6961 gaggaaatcc ggaacgccgt cgagacaggc tcggcggaca ggatagagga tgagatggga
     7021 gacctgctct tcgccgccgt gaacttggcg aggcatcttg aggtcaaccc cgattcggcg
     7081 ctcggaaggg cgaaccggaa gttctccgac cggttccgga tcgtcgagtc catggtgcgc
     7141 gaaacaggac gcccatggag cagcttttct cttgatacgc tggatacttt ttgggaacag
     7201 gcgaaaaaat ccctgtaaca gggaaactga tcgtgtacag gaacacaaaa aattgaacgt
     7261 atggaggaaa tgcgcacatg accaccccga acaagaaaaa gagcgagacg gcccccgcag
     7321 catcttccac gtcgcataag gagacgacta tggagacaaa gagcgaaccg ctgccgaaga
     7381 aagagtcgcg gcagatcaga aaggtcggca ttaccgacac gacgcttcgc gacgcgcacc
     7441 agtcgctcat ggcgacgcgt atgaaggtcg aggatatggc cgagatcatc gagcggatgg
     7501 acgacatagg gttccattcg atggaggtct ggggaggcgc cacctacgac agctgcatgc
     7561 gcttcctcga cgaagacccc tgggagcgcc tgcgcaccat gcggagactc ttcaagaaga
     7621 cgaagcttca gatgctcctg cgcggccaga acgtcgtggg ataccgccac tacgccgacg
     7681 acaccgtgcg cgagttcgtc aagagggccg tcgggaacgg actcgacatc atccgcgtct
     7741 tcgacgcgct caacgacctg cggaacatgg aggtcgcggc cgatcagatc aagaaggaag
     7801 gagcgcatct gcagcttgcc ttctcgttca ccctttctcc ggtgcactcc ctggacgcct
     7861 tcggcaagct cgcaaaggac atgaagtcca tgggcgcgga ctccatctgc atcaaggaca
     7921 tggcgggcat cgtcagcccg gtggagactg ccgcgcttgt caagacgatc aagaaagaga
     7981 cgggactccc cgtgcagata cactcccact acaccagcgg catggcctcc atggcctatt
     8041 acgccgggct cgaggcggga gcggacgtcg tcgactgcgc catctcgccg tttgccatgg
     8101 gcacgagcca gcctcctgtg gagtcgatgg tggccgcgct caagggcggc gatttcgaca
     8161 cggatatcga gctcgaaaag ctcgtgccga tcgcgatgca cttcaagaaa gtgcgcgaca
     8221 agtactccaa gatcttcgtg gatttgggcg gcgtggacat caacgtgctc atctaccaga
     8281 tccccggagg catgtactcc aacctcgtga gccagctcaa ggatcagaac gcccttgccc
     8341 ggcttcccga ggtgctcaag gaagtgcccc gcgtccgcga ggagatgggg taccccccgc
     8401 tcgttacgcc caccagccag atcgtgggca cgcagg
//
LOCUS       DAOTIL010000016         8418 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminobacteriaceae bacterium isolate AMR_MDS_3026
            NODE_10151_length_8418_cov_3.728566, whole genome shotgun sequence.
ACCESSION   DAOTIL010000016 DAOTIL010000000
VERSION     DAOTIL010000016.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868050
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminobacteriaceae bacterium (wastewater metagenome)
  ORGANISM  Aminobacteriaceae bacterium
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae.
REFERENCE   1  (bases 1 to 8418)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8418)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:19:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,224
            CDSs (total)                      :: 3,198
            Genes (coding)                    :: 3,184
            CDSs (with protein)               :: 3,184
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8418
                     /organism="Aminobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10151_length_8418_cov_3.728566"
                     /isolate="AMR_MDS_3026"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:3034609"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(119..673)
                     /locus_tag="P5201_00665"
     CDS             complement(119..673)
                     /locus_tag="P5201_00665"
                     /EC_number="3.2.2.15"
                     /inference="COORDINATES: protein motif:HMM:TIGR04274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033958 - DNA-deoxyinosine glycosylase
                     activity [Evidence IEA];
                     GO_process: GO:0006285 - base-excision repair, AP site
                     formation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-deoxyinosine glycosylase"
                     /protein_id="HRV97074.1"
                     /translation="MPVVSSFPPVARTDAVICILGSMPGRASLEADQYYAHPRNAFWR
                     LLESVLGIPCSLPYPERLERLKERRTALWDVLGTCARDGSLDSRIEPSSIIPNDFATF
                     FAMHPLIGTVFFNGGKAEAVYRREVLPTLAEPARSIRLLRLPSTSPAHAGMTFEQKKD
                     AWGRLGRVLEENASQGYHERGNDC"
     gene            complement(710..2014)
                     /locus_tag="P5201_00670"
     CDS             complement(710..2014)
                     /locus_tag="P5201_00670"
                     /inference="COORDINATES: protein motif:HMM:NF024606.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TerB N-terminal domain-containing protein"
                     /protein_id="HRV97075.1"
                     /translation="MEFIVFLVIAALLVRFIKSYFGAPKRDEILVIPGDAENYRSGSK
                     NASSQWIEPGQSFICRGYSIDDGMVYVGERMRDFTGSNEGCLINPELDVAPEGTSSLP
                     VQQIQPRYETMSPEQRGAFLRWLAEGRFGRADPAWPFLFFYGIERRLFVDGPRGYVSH
                     QERNELVLEVRRLLHVYGEHRSFRGYCKNLLATEWVLKGDYAEIPEWVDFTDRFCAEA
                     FNSIISLYVSRDQPIPGEVALQWLFLHPEHISGTLSRREPEKFEHLFLAGYKEKFGEG
                     ILIHKNRSPLNLIYKGANPSLGNGVKLTVPSLPDPFLLATPIKKLDLLAEECIKAMET
                     DDTLEFKGQVFEADGVDPLESLDDIHSEFMARLATKMLWPRADVLAVCDALSIAPEPS
                     FELLNAWGRIAGGLPLIDDGDPVFVDIPLLQEMLLRENADAQ"
     gene            complement(2123..3157)
                     /locus_tag="P5201_00675"
     CDS             complement(2123..3157)
                     /locus_tag="P5201_00675"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRV97076.1"
                     /translation="MIIGRDDLRSAAKKGILSEEQADSLWDFLSEEMREVPSFRMAHI
                     LYYLGGLIAIGAMTLFMNRGWELFGGWGIFAISLAYALVGLFFLAYFMKKPGLSIPAG
                     ILGAFVAVMVPLAVYGLQNALGYWEGNRIYREYHVYIDWRWLSMELATLAVAVGLLWR
                     WKLPFMLMPVALTLWYMSMDLAPFLFKNEDLTWELRRTVSLWFGLAVLCFAVFVDFRT
                     RSRKDYAFWLYLCGLAAFWGGLSLMDSDSELGKFVYFCINIFLIAVGALLSRRAFAVF
                     GGFGAAGYIGHLTWRVFSDSVVFPFVLSFAGFFVIWLGIRWQKKEKQITALLRSFLSP
                     EMRSFLERNE"
     gene            3358..3783
                     /locus_tag="P5201_00680"
     CDS             3358..3783
                     /locus_tag="P5201_00680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008520118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HRV97077.1"
                     /translation="MIAKKGSKVKVHYTGTLKNGDVFDSSEGRSPLEFTVGAGQMIPG
                     FDKGVEGMTAGEKKTITLPPKEAYGEKRDDMFFRVSRDVLPEGYTPSIGDPLRVATDD
                     GGVLNVFIAEMDEDGITLDGNHRLAGEELTFEVTMVEIA"
     gene            3968..5278
                     /locus_tag="P5201_00685"
     CDS             3968..5278
                     /locus_tag="P5201_00685"
                     /EC_number="4.1.99.3"
                     /inference="COORDINATES: protein motif:HMM:NF013071.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribodipyrimidine photo-lyase"
                     /protein_id="HRV97078.1"
                     /translation="MSREQRAEDSWPLLAAAHAAASLDRPLICLFCFAPSFAHAQKRH
                     YAFLIQGLKETSRQLRKYNTQMKILYGEPGPTVAALTATLDAALVFIDFDPFPEKQPW
                     RKEFLDRVSTTVYEVDGHNIIPAWIVSPKKEYSAATFRRKVHPLLDSYLEEYPLFPEL
                     PPRESIETPLFLSLEDAAARVATETAEAPLSPYSPGSSAARAAMRRFFSERLNDYDTR
                     RNNPALSALSDLSPWLHFGQLSPQRVALEALKTPQSPAREAFLEELIVRRELADNFCF
                     YEPMAGTYDGLPEWGRKTLALHAGDRRPRLYTMAELEEARTGDPLWNAAQKEMVWKGK
                     MHGWARMYWAKKILEWTETPEEAFERVLILNDRYELDGRDPSGVVGVSWAIGGLHDRP
                     WAERSVFGMVRYMNDKGAARKFRVADYIESVNAFISNRTGENLS"
     gene            complement(5498..6088)
                     /locus_tag="P5201_00690"
     CDS             complement(5498..6088)
                     /locus_tag="P5201_00690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013049222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin transporter BioY"
                     /protein_id="HRV97079.1"
                     /translation="MRYKGWILAGFFAGLTVVGTFLRIPFPLVPLTLQVLMVLLAGFT
                     LGPKYGMLSQVIYLFIGLAGLPVFSGGGGIGTVFSPTFGYLLSYPAAAWIVGRISSKE
                     GANAIRFVVASCAGIAFIYLLGASVLYLNLKFLAGKSIGVYQVVKIGVLPFLLPDLLK
                     GGVAALFALKIRPLLGTSGAELRRAPLHGRLTRYKP"
     gene            complement(7580..8200)
                     /locus_tag="P5201_00695"
     CDS             complement(7580..8200)
                     /locus_tag="P5201_00695"
                     /inference="COORDINATES: protein motif:HMM:NF013378.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YigZ family protein"
                     /protein_id="HRV97080.1"
                     /translation="MQVDYKEPASETTVEVKINKSRFLGHVRICRSEEEVRMKLKEVC
                     DLHKQATHNCWAYRIGEESYREYFSDDGEPAGTAGKPILGAISRLDLTNALIVVTRYF
                     GGIKLGVRGLIEAYGSTATNALNASGMAECTIKSQYQIVAPYDMVKTLHRLLFSLGAV
                     EKDVLFDYGERVSILCSLPRSSGKDFESALDELLNMSRIHSWNGVQ"
     gene            complement(8200..>8418)
                     /locus_tag="P5201_00700"
     CDS             complement(8200..>8418)
                     /locus_tag="P5201_00700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014163987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HIT family hydrolase"
                     /protein_id="HRV97081.1"
                     /translation="CIVVLRKTMNPQGFNLGVNLGKVAGAGFDGHLHLHIVPRWNGDT
                     NFMPVFADVRVVAEELAETYRKMKEAWD"
BASE COUNT         2258 a         2290 c         2045 g         1825 t
ORIGIN      
        1 accaacgcag tgtaccgaca tccattacaa accctccctt tgttatgatc catcgaatca
       61 ggagaccacc tgcggtcacg gaggtactcc cgggaagatt gatatgctca ctctctcctc
      121 agcaatcgtt cccccgctca tggtaccctt gactggcgtt ttcttcaagg actctcccga
      181 ggcggcccca cgcatccttc ttctgctcga aggtcattcc tgcatgcgcg ggacttgtag
      241 aggggaggcg gagaagtcga atggatcggg caggttcggc aagagtcggg agtacttctc
      301 tgcgatagac cgcctcagcc tttccgccgt tgaaaaaaac agttcctata agaggatgca
      361 tggcgaaaaa agtcgcaaaa tcgttgggaa tgatactgga cggttcgata cgtgaatcaa
      421 ggctcccgtc gcgagcgcac gtcccaagga cgtcccagag cgctgtacgt cgttctttca
      481 gccgttcaag cctctccgga tatggcaacg agcagggaat acccagtacg gactcaagaa
      541 gacgccagaa ggcgtttcga gggtgcgcat agtactggtc ggcttcaagc gacgctctgc
      601 cgggcatact gccgaggata cagatcacgg cgtccgtccg ggcgaccggg gggaaacttg
      661 aaactaccgg cacaaaataa ccttcctctc ttgagtcgct cccggcacgc tactgggcat
      721 ctgcattctc ccgaagcagc atctcctgca acaaagggat atccacaaaa accggatccc
      781 cgtcgtcaat caggggcagt cccccggcta tccgccccca cgcgttcagc agctcgaagg
      841 acggttccgg cgcaatggaa agggcgtcgc agaccgccag aacatccgct ctcggccaga
      901 gcattttcgt cgccaggcgg gccataaatt cgctgtggat atcgtccaga gactcgagag
      961 gatcgacgcc gtcggcctcg aacacctgtc ctttaaattc aagagtgtca tcggtctcca
     1021 tcgcctttat gcactcttcg gcaaggaggt cgagtttctt tataggcgtt gcaagaagaa
     1081 agggatccgg tagagaagga actgtgagct tgacaccgtt tcccagcgaa gggttcgctc
     1141 ctttatagat aaggttcaag ggcgaacggt tcttatggat aaggatgcct tcgccgaact
     1201 tttccttgta tcctgcgagg aaaagatgtt cgaacttctc cggttcgcgg cgcgaaagcg
     1261 tccccgaaat atgttcagga tgcagaaaga gccattgaag cgccacttcg ccgggaatcg
     1321 gttgatcccg cgaaacgtag agcgagatga tggagttgaa cgcctcggcg caaaaacgat
     1381 cggtgaagtc gacccattcg ggaatttccg cataatcccc tttgaggacc cattcggttg
     1441 caaggaggtt tttacagtat ccccggaatg aacggtgctc gccgtagaca tgcagcagac
     1501 gccgcacttc caggacaagc tcgttgcgtt cctggtgaga gacgtaccct ctcggtccgt
     1561 ccacaaagag ccgccgttcg ataccgtaga agaagagaaa gggccatgcg gggtccgccc
     1621 ggccgaatcg cccttctgcg agccagcgta gaaaggcccc gcgctgctcg gggctcatcg
     1681 tctcgtaacg gggctgtatc tgctgtaccg gcaacgagga ggtaccctca ggcgcaacgt
     1741 ccagttccgg attgatgaga cacccttcgt tcgagccggt aaaatcccgc attctttcac
     1801 cgacatacac cattccatca tcaatcgaat accctctgca aatgaaagac tgtcctggct
     1861 cgatccactg ggaagaggca ttcttcgaac cgcttctgta gttttccgcg tctcccggaa
     1921 tgaccagaat ttcgtcgcgc ttcggagctc caaagtagga tttgataaag cgaaccagaa
     1981 gtgcggcgat cacaagaaag acaataaact ccacgttttt ccctcttttc agcagggctt
     2041 caaagcaaca ggcccttcac tagatttccg cggattcctt cgcggccggc gcctctccga
     2101 atcgtcacgc tctcggaggg aatcactcat tcctctcaag aaaactccgc atttcggggg
     2161 agagaaagga gcgcagcagc gccgtaatct gtttttcctt cttctgccag cgtattccga
     2221 gccagatgac aaaaaaaccg gcaaacgaaa gaacgaacgg aaagactacg ctgtcgctga
     2281 acactctcca tgttaaatga ccaatatacc cagcggcccc aaatccgccg aagacggcaa
     2341 aggctcttcg tgagagaagg gctcctacgg caatcagaaa gatgttgatg cagaagtaga
     2401 caaacttccc tagttcgctg tccgagtcca tcagagagag cccgccccag aaagcggcga
     2461 ggccgcacag atagagccaa aacgcataat cctttcgtga acgcgtacgg aaatcgacga
     2521 ataccgcaaa acagagcact gcgaggccaa accagagcga aaccgtccgt cgcagttccc
     2581 aagtaagatc ctcattcttg aagaggaagg gcgccaggtc catgctcatg taccagaggg
     2641 tcagcgcaac cggcatgagc atgaagggaa gcttccagcg ccataaaagt cccaccgcga
     2701 ccgcaagagt tgccaattcc atggacagcc agcgccagtc gatgtacacg tggtactccc
     2761 tgtatattct gttcccctcc caatacccca gcgcgttctg aaggccgtat accgcaagcg
     2821 gcaccatcac cgccacaaat gcaccgagaa tacccgcggg aatggagagg ccgggttttt
     2881 tcatgaaata cgccagaaag aagagaccta caagcgcata ggcaagtgag atagcaaaga
     2941 tgccccatcc tccgaaaagc tcccaacccc gattcatgaa gagcgtcatc gcaccgatcg
     3001 caatcagtcc gccaagatag tagagaatat gcgccatgcg aaaagagggg acttctcgca
     3061 tctcttcgga aagaaaatcc catagggaat ccgcctgttc ttcggagaga atcccttttt
     3121 tcgctgcact tctcaaatca tctctgccga taatcatctc tcactcacct cccgggaaag
     3181 atttgccgtc atattcgtat tctacctcat aatcggacgc tttccagagc ttcttcaaaa
     3241 tacttcgacg gtagattttt ttctccggat gaaatagact ggttgaagag agcgcatcgg
     3301 ggttctttca tccgggaaat gcgcgcttcc ggttcggacc ggagaaagga gcaacgaatg
     3361 attgcgaaga aaggttcaaa agtgaaagtc cactatacgg gcactctgaa aaacggggat
     3421 gtcttcgact cctcagaagg acgatctccc ttggagttca ccgttggcgc cggacagatg
     3481 atccccgggt ttgacaaggg ggttgaaggg atgaccgcag gagagaaaaa gaccatcaca
     3541 ctccccccca aagaagccta cggggagaag agagacgaca tgttcttcag agtctcgcgc
     3601 gacgtcctcc cggaaggata cacgccctcc atcggcgatc ctctcagagt agcgacagac
     3661 gacggcggcg tactcaacgt ctttatcgcc gagatggacg aggatggaat cactctcgac
     3721 gggaatcacc gccttgccgg cgaagaactc acatttgaag taacaatggt agaaatagcc
     3781 tgagcaacgc caccaaataa aagaggaggc cgcagcgagg agacacgccg cgcctcctct
     3841 tttatttccc gggaaataca actgaaagga gagatcaact atgccctttc aattttctga
     3901 accattttca cgaagagcac atctgcttca aaacggaagt caaggcaaag gtcctgttct
     3961 ctattggatg agccgcgagc agcgcgcaga agacagctgg cctctcctcg ccgcggcgca
     4021 cgcggctgct tcgcttgacc gccctctcat ctgccttttc tgtttcgctc cttccttcgc
     4081 ccacgctcag aagcgacact atgccttcct tatacaaggt ctaaaagaaa catcccgaca
     4141 actacgcaaa tataacacgc aaatgaaaat actatacgga gaaccaggcc ctactgtcgc
     4201 tgcactcacg gcgacgctcg atgcagcgct tgtcttcatc gatttcgacc cgttcccgga
     4261 gaagcagccg tggagaaagg aattcctcga cagagtttcg acaacggtct acgaggtcga
     4321 cgggcacaac ataatcccgg cgtggatcgt atcgccgaag aaggagtaca gcgccgcgac
     4381 atttcgcaga aaagtacacc ctctgcttga ttcctatctc gaagagtacc ctctgtttcc
     4441 ggaactccct ccgcgagaga gtatagaaac gccgcttttt ctctctcttg aagatgctgc
     4501 cgcccgagtc gcaacagaaa ccgcagaagc gcccctttcg ccttactcgc ccggctcttc
     4561 cgctgcccgc gctgccatgc gccggttctt ttcagaaaga ttgaatgact acgacactcg
     4621 aagaaacaat ccggcgcttt ctgccctctc cgatctttcg ccgtggctcc acttcggcca
     4681 gctctccccg caaagagtcg cactggaggc gctgaaaaca ccccaatccc ccgcgaggga
     4741 agcctttctc gaagaactca tagtccgtcg agagctggca gataatttct gtttttatga
     4801 gccaatggcc ggaacctacg atggactccc tgaatggggc cggaaaacgc tcgcgcttca
     4861 tgcgggcgac cggagacctc gtctttacac aatggcggag cttgaagaag cccgaaccgg
     4921 cgatcctctc tggaacgctg cgcaaaaaga gatggtctgg aagggaaaga tgcacgggtg
     4981 ggcgagaatg tactgggcga agaaaatcct cgaatggacg gagaccccgg aagaggcctt
     5041 tgaacgtgtg ctcattctca acgaccggta cgagcttgac ggacgcgacc ccagcggcgt
     5101 cgtaggcgtt tcatgggcaa tcggcggtct tcacgacaga ccttgggctg aacgctctgt
     5161 tttcgggatg gtacgctata tgaacgacaa gggagcggcg agaaagtttc gtgtagccga
     5221 ctatatcgag agcgtcaacg cgtttatatc gaaccgaacg ggagagaatc tcagctaaga
     5281 tcagtattag tcaacgtcta tgaacactac tttgtttaaa attctgctca tcggaagcat
     5341 gtagatcgtg atattggaga cacgctcctc tttctgcaac gaagatgagg gcggcccccc
     5401 ccatttcaaa cataacacaa tgccgccgga gaaaggcctg agtcctctca actggcggca
     5461 ttgtcattcg ttcattgaac accgggattc cggtctgtta cggcttgtat cgtgtaagcc
     5521 tcccgtgcag aggcgctctc cgcaactctg caccgcttgt accaagaagc ggacgtattt
     5581 tcaaagcaaa cagcgcggcc actcccccct tgagaagatc agggagtaag aagggcagaa
     5641 caccgatctt cacaacctgg tacacaccga tgctctttcc ggccaaaaac ttcaggttga
     5701 gatagagcac tgaagcgccg agcaaataaa taaacgctat ccccgcacat gaggcaacaa
     5761 cgaaacggat cgcgttcgct ccttccttgg aagagatccg gccaacgatc catgcggccg
     5821 caggatatga gagaaggtag ccaaacgtcg gagaaaagac cgttccgatc cctcccccgc
     5881 cgctgaaaac cgggagtccc gcaagtccga taaacaggta gatgacctgt gatagcatgc
     5941 cgtatttcgg tcccagcgta aaaccggcga gaagcaccat taatacctga agcgtcagcg
     6001 gaaccagagg gaaaggaata cgaagaaacg ttccaacaac tgtaaggccg gcgaaaaaac
     6061 cggcaagaat ccaccctttg tatctcatgg agcaccccct cagctatgtc acccggatat
     6121 tttagtggtt gacatagctt tttgcaacgt atttccgcct cctcgtctgc ttctctctta
     6181 aaggaagcca tattccgttg tcagcgaaca ggcgaagtat atgttctctg ggaaaaatct
     6241 ttcacgttcc tgtaaacagt ctctctgatt tctagtcttc gcgagaaaga aacaccataa
     6301 ttgcgtatga agaagctgat ctatactgac ctacaccctc caaataggtc tctttgtatc
     6361 gaaaggatgt tccaacagtt gacaacgttc accctcacca gtctgaaggc acacttccgg
     6421 aatagcgtac aaccgctaat ttgcagcgca tgcaatcacc tgtaacaatt cacaaagtct
     6481 ccaactacaa agaggccccc aacgtgcctc tcaaagcagc gcactataac aaagatacag
     6541 agcattttcc gcgatgtctt gggtacagcc gatcccggcc gcgcatgcaa ttgatacaat
     6601 cggccgcatg aaaggataaa tgggttttcg gactcttttg tctttcgaac cacacattga
     6661 atgctattta gcgtgcagaa tgtcacttca atagaggatc cacgtgacag gaagaaagaa
     6721 gccaaatgga cgtgtacaag aattccacca ctacttctca atacgatgag taaggaacag
     6781 agatgcacgc atttcgacca gacacgaaat gtggacgtgt ggcgcgttaa tcagactgtc
     6841 tcattgactc ataacatcat caaccagaaa gagaagaaac aaagattctt tccaagagat
     6901 ccactttggt tctatcagaa ctttttcatt caacaagaag agtttcggaa gggaagaaag
     6961 caggaaattt cgatgaaaag aacgaaagaa ttttggctct taatcaaaac aaatacgacg
     7021 atacacttag gacatccgga agaagacaca cgaaagtgcc attatgattg aattatgttt
     7081 agtggtgagc tcgagtatcg aaggcgaagg gaacaactaa agaaatctac tttttacgag
     7141 ttcgaggaag gagtttgttt ttccgtgatt ccattccaaa tatggactac aattcctgga
     7201 actcattctt aaatgaaaac ggttctattg agactctttg ctgagtctca atacgaaaag
     7261 gagaaagcgt tgataaccgg agctgctgtc agatgatcaa ttttcggctg tcgttcgctc
     7321 aaaaagtgat caatcttagt gtaccaaaac caagcgatat atgaaaaaga ctcatgtgtt
     7381 gtagaaagtt actctccaga gaattcgcgg cgaaaaatgt gattcttcaa caaaggaaac
     7441 ccagaatact gtcgagtcag actcgtagga actcgttcca atcgagctcg atgaagaaag
     7501 aagaccgttg aatcagatct tctcaaagat tgaaagggtg gcaaaaaaaa cggaacggcg
     7561 cccggaagaa tgaagaagtc tactgaacac cgttccatga atgtattctg ctcatgttca
     7621 agagctcatc aagcgcgctt tcaaaatcct ttccggagga tcgaggcagc gaacaaagaa
     7681 tactgacacg ctctccgtag tcgaagagaa catctttctc gactgcgcca agtgaaaata
     7741 acagccggtg caatgttttt accatatcgt acggagcaac tatctggtac tgactcttta
     7801 tcgtacattc cgccataccg gaggcattca gcgcattcgt cgcagtggag ccgtacgctt
     7861 cgattaaacc gcgaacacca agctttatgc ctccgaaata tcgagtgact acaataagag
     7921 catttgtcag atcaagtcgt gaaattgctc caaggatcgg tttccccgct gtccctgcgg
     7981 gttcgccgtc atcggaaaaa tactctctat atgactcctc gccgatcctg tatgcccagc
     8041 aattatgcgt tgcctgctta tgcagatcgc acacctcctt gagcttcatc cgaacttctt
     8101 cctcggatct gcaaatacgg acatggccca agaatctgga tttgttgatt tttacttcta
     8161 ccgtagtttc cgaagctggt tctttgtaat caacctgcat caatcccaag cttccttcat
     8221 ctttctgtat gtttctgcca gttcctccgc gactactcgt acatctgcaa atacaggcat
     8281 aaaattcgta tctccgttcc atcgaggaac aatgtgaaga tggagatgac cgtcaaaacc
     8341 agcccccgcc acctttccaa gattcacgcc aagattgaaa ccctgcgggt tcatcgtctt
     8401 tcggagcacc acgatgca
//
LOCUS       DAOTIL010000017         8342 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminobacteriaceae bacterium isolate AMR_MDS_3026
            NODE_10285_length_8342_cov_3.784120, whole genome shotgun sequence.
ACCESSION   DAOTIL010000017 DAOTIL010000000
VERSION     DAOTIL010000017.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868050
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminobacteriaceae bacterium (wastewater metagenome)
  ORGANISM  Aminobacteriaceae bacterium
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae.
REFERENCE   1  (bases 1 to 8342)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8342)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:19:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,224
            CDSs (total)                      :: 3,198
            Genes (coding)                    :: 3,184
            CDSs (with protein)               :: 3,184
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8342
                     /organism="Aminobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10285_length_8342_cov_3.784120"
                     /isolate="AMR_MDS_3026"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:3034609"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            114..614
                     /locus_tag="P5201_00705"
     CDS             114..614
                     /locus_tag="P5201_00705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013047503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter small permease"
                     /protein_id="HRV97082.1"
                     /translation="MSFFRKTLDRVNALAEYAVCALLAVMVVVVFLQVIFRFIIRSSL
                     PWSEELSRYVMVWIVFLGASIGIKRKSHIGVEAVVAMLPDSMRRWTAILVNALCCVFF
                     VFMVHYGRMILRVVSSQLSPAMEISMAIPYSAVFVGGTLMFVHCFFQMVELMVEALSG
                     RAVKSA"
     gene            611..1891
                     /locus_tag="P5201_00710"
     CDS             611..1891
                     /locus_tag="P5201_00710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013047504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0031317 - tripartite ATP-independent
                     periplasmic transporter complex [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter large permease"
                     /protein_id="HRV97083.1"
                     /translation="MTGVLFGSLVFFFLLNVPIAVAIGLASLSAILWSGAIPPVVLVQ
                     KMFTATDSFPLMAVPFFILAGSLMEFGGISRRLVDFANSIVGRFSGGLAFVAIIASMF
                     FGAISGAAVACVAAIGTILIPAMVRRGYDTPYATAVQATAGTLGVMIPPSIPMIIYGV
                     LTGVSIGALFMGGILPGILVGGSLMLVAWMIARKRGYKGDEKASGAKIWATFKDAILA
                     LLMPVIILGGIYGGVFTPTEAAVVAVAYGFIIGFFVYRELSLSKLKDILVNTSVGTAT
                     IMFIIATSSVFSWILTAQRVPQAVAEAILSISTNPIVILTLINLLLLFIGTFMETVAS
                     IIILVPVLLPVVTQIGVDPLHFGIIIVVNLAIGMVTPPLGVCLFVGCGISGITLESIS
                     KAVWPFILSMIVAVLLLTYIPWISIILPRLAGIY"
     gene            2227..3399
                     /locus_tag="P5201_00715"
     CDS             2227..3399
                     /locus_tag="P5201_00715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005659956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HRV97084.1"
                     /translation="MKEVVVGVVGGGFASHFHSRCFARVSGFSVRLKAIADVEIEKAK
                     AVAEAYRYEEYHDDYSALLRDPEIDVVDICTPPFLHPRITMEAMEAGKHVICEKPLAG
                     YFGSPGDIEPIGDTVPKSRMYQSVLTEIETMKAALEGSDRLFMYAENYVYSPNILKAA
                     EIVRAKKSKILFMKGEESLKGSTSPVAGRWDKTGGGSLIRVGCHPLGGILWLKREEAR
                     AGGAQIGVHSVLCDTGRMTPGLDEYERRHISVRPVDVEDYASVALTFSDGTKALVIAG
                     DTMLGGTRNYVEVYANDAVMHCRITPSDVMSTYMLDEEGLENVYISEMLPAKTGWQQP
                     FVAEEHLRGYAAQFQDFIECAATGRAPLSDFELACETIRVIYAAYLSAEEGRRILL"
     gene            complement(3530..4468)
                     /locus_tag="P5201_00720"
     CDS             complement(3530..4468)
                     /locus_tag="P5201_00720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008709474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylglutaryl-CoA lyase"
                     /protein_id="HRV97085.1"
                     /translation="MTMTLPKSIFFCEVGPRDGLQNEPATLSVERKVRLIEGVVDAGV
                     RIVEIGSYVNPKAVPQMADTDEVARAIRRMEGVEYRALVANLKGVERALASGVNKVKL
                     TVSASESHAKANLNKTPSELIDGFKSCTEFARSNGAEVSGAISTAFGCPFEGKVPPEK
                     VAEIAERLHDLGITELSLSDTTGMANPRQVYDLATSMQEMLPDVRWFLHFHNTRGMAL
                     ANTLAGLQAGIVRYDASLAGLGGCPFAPGASGNVASEDMIHMLHEMGVDTGIDLDKLL
                     DLARELRGWVGHDTDSALLKAGPCCRPIPRDGAAKQ"
     gene            4796..6595
                     /locus_tag="P5201_00725"
     CDS             4796..6595
                     /locus_tag="P5201_00725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008709503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-binding protein"
                     /protein_id="HRV97086.1"
                     /translation="MRKDLVAFQLVKFLEARGVEKVFGLCGHTVIGFLDALKESKITF
                     ITVRHEQIAAHAADGYARGKGCRVPGVLLTHLGPGLANATTGVAEAGLNSIPMVVIAG
                     DVPSCYFGRHPHQEVNLHADASQYEIYRPFVKRAWRVDRPELLPEVMDKAFRLAVTGR
                     PGPVLVSVPMDIFSMEIDTRFFEQRMHNMPLLPRPGLDVKTAEDIAALLAEAKSPVLY
                     PGGGVISSGAASALTELATFLEIPVLYTLMGKGSIPDDHPLAVGMTGFWGTEFNNSAA
                     MKADVMMAVGTRLSEADCSSWYFGETFDVPPTKLIHIDINQEEIGRNYTTEIGAVCDA
                     KMALDAILAAAKRKYPNGVKRPELIGEIAEAKAAYRATIAEAQKSDQFPMRPERILAD
                     LREALPRDGFVVADVGWNKNGVGQQFDIYEPGTFVAPGGLATMGYGPSAALGVKVACP
                     DRKVVALIGDGGMGANVSPFATAAVENIGVVWVVMNNCAFGTIAGLERQHYDHEFGTL
                     FEREGKPYSPDFAAIARAYGIDGYTVERAEDFKPMLEKALASDKPCVLDVRMENAPVV
                     TKGCWNINDIFRKRGEEKPWRVWSWEEIEPWRM"
     gene            6674..8095
                     /locus_tag="P5201_00730"
     CDS             6674..8095
                     /locus_tag="P5201_00730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008709504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HRV97087.1"
                     /translation="MAKKELTEAQRQTLETVFARARAAEKIIENYDQERVDRMCRCIA
                     WAAANPRTFDRFCKMGVEESGAGDWSGRYGKRHKILGVLRDALRQKSVGIVEVNPEKG
                     LVRYGKPAGLITSLIPMTNPELTPIVTAIYALKARDVVVFSPHPRTRKTTFEVVESMR
                     QGLKSIGEPEDFLQCITEVNMTIVEELMKMGDLVMATGGLAMSRAAHSSGKPAYCSGA
                     GNATMIFDETADVEEAARNTRISKTSDFGSGCSADGNLVIHGSIYEKMVAQLVKEGGY
                     LANDEEREKLKKAMWDDEGHRIVETVAVAPQKLCKAAGFEIPEDRKFVMVVGEGIGKE
                     FPFSGEKLTTLLALYRYEGEFENALKMIGEIYKVGGRGHSCGIYSFNEEHIHALALRA
                     PVTRIMVRQPQSKANAGSANNGMPMTSSMGCGTWGGNQVSENIALKHYMNSTWVAKPI
                     LTDAPSEEVLFGEFYDPSNTREG"
BASE COUNT         1702 a         2225 c         2577 g         1838 t
ORIGIN      
        1 aagtaatctt ctcccggtat tgaaccggta ttccgccgaa gggggagttg tccttcagtc
       61 ttccttcggc gggcctttcc ggagattatt gtctgagcga aaggggggca tttatgtcct
      121 ttttcagaaa aacactcgac agggtgaacg ccttggcgga atacgcagtg tgcgctctcc
      181 tcgcggtgat ggtcgtggtg gtgttccttc aggttatttt ccgtttcatc attcgctctt
      241 cgcttccgtg gtcggaagag ctgtcccgat acgttatggt atggatcgtt ttcctgggag
      301 cgagcattgg catcaagcgg aaatcccata tcggcgtgga ggccgtggtc gcgatgttgc
      361 cggattcgat gcgccgctgg acggctattc tcgtcaacgc gctgtgctgt gttttcttcg
      421 ttttcatggt ccattacggc aggatgattc tcagggttgt gagttcgcag ctctctcctg
      481 caatggagat atcgatggcg attccctata gcgcggtttt tgtcggcgga acgttgatgt
      541 tcgttcactg tttctttcaa atggtggaac tgatggtcga agctctttcg ggaagggcgg
      601 tgaagagcgc atgaccggag ttctcttcgg aagtcttgtc tttttctttc tgttgaacgt
      661 tccgattgcg gtggccattg gattggcctc gctctcggcg atcctctggt ccggcgcgat
      721 cccgcccgtg gtgcttgtgc agaagatgtt caccgcgacc gactccttcc ccctcatggc
      781 ggttcccttc ttcattctag ccgggtcgtt gatggagttc ggaggaatct cccgccgcct
      841 ggtcgatttc gcaaactcaa tcgtcgggag gttttcaggc ggactcgctt tcgtcgccat
      901 catcgcaagc atgtttttcg gcgcgatctc cggagccgca gtagcctgcg tcgccgccat
      961 cggaacgatc ctcattccgg caatggtccg gcgcggatac gatacgccct acgccactgc
     1021 ggtgcaggcg actgcgggaa cgctcggcgt catgatcccg ccgagcattc cgatgatcat
     1081 ttacggcgtg ctcacgggcg tctccatcgg cgcgttgttc atgggcggca tcctccccgg
     1141 tatcctcgtc gggggatccc tgatgctcgt tgcgtggatg attgcgcgca agagggggta
     1201 caagggcgat gaaaaggcaa gcggcgcaaa aatatgggcg accttcaagg atgcgattct
     1261 cgcgctcctg atgccggtca tcattctcgg cggcatctat ggaggagtct ttaccccgac
     1321 ggaagcggcg gttgtcgcgg tggcctacgg cttcatcatc ggatttttcg tctaccggga
     1381 actgagcctc tcgaagctga aggatatcct ggtgaacact tcagtcggca cggcgacgat
     1441 catgttcatc atcgcgacct cgtcggtctt cagctggatt ctgacggcgc agagagtacc
     1501 tcaggctgtg gccgaggcta tcctctcgat ctcgacgaac ccgatcgtca tcctcacgct
     1561 catcaacctt cttttgctct tcataggcac cttcatggag acggttgcct ccatcatcat
     1621 cctggtcccc gttcttcttc cggtagtgac gcagataggc gtcgatccgc tccacttcgg
     1681 catcattatt gtggtgaacc ttgccatagg aatggtcacc cctccgctcg gcgtctgcct
     1741 cttcgtcggt tgcggaatca gcgggatcac gctggaatca atctccaaag cggtgtggcc
     1801 gttcatcctg agcatgattg tcgcggtgct actgctgacg tatatccctt ggataagcat
     1861 cattcttcct cgtctggcgg ggatttatta gaaaacttgc tgcacgcaaa agcgcctcga
     1921 aacgtatcgt ttcggggcgc tttttcaatt ggactaaaga gatcgcgata ctcgaacgga
     1981 tacgccaagt cagcgcaagc gtgtcgataa atcaacaaat cgctcttgtt atgctgatct
     2041 ttgaatatgg aaatagtctt gacagggtat gccgtgtttt gtattgtaac ctcgaagtcc
     2101 ttttagcgga tgttattccc gtagagcggg atatggtgcg cttttttgag cggagaatcc
     2161 tctgccctcg ctcggagagc tcttttttcc tcttactttt tcttcacaat cgcgaggtga
     2221 catagaatga aagaagttgt tgtgggcgtt gtcggaggcg gattcgcctc gcattttcac
     2281 agcagatgtt tcgcaagggt ttcggggttt tccgtgcgtt tgaaagccat cgcggacgtc
     2341 gagatcgaaa aggcgaaggc cgttgcggaa gcctatcgtt acgaggagta ccatgacgat
     2401 tacagcgcgc ttttgcgcga tccggagatc gacgtcgtcg atatatgcac tccccctttt
     2461 ctgcacccgc gaatcacgat ggaggcgatg gaggcgggga agcatgtcat ttgtgaaaaa
     2521 ccgctcgccg ggtacttcgg ctctccggga gacatcgaac cgataggcga tactgttccc
     2581 aagtccagaa tgtaccagtc cgtgttgacg gaaatagaaa caatgaaggc ggcgcttgaa
     2641 ggaagcgaca ggctctttat gtacgccgaa aactacgtct actcgccgaa cattctgaag
     2701 gccgccgaga tagtgcgggc gaagaaaagc aaaattctct tcatgaaggg cgaggaaagc
     2761 ctgaagggat caacctctcc agtcgccgga cgatgggata aaaccggggg cggttcgctc
     2821 atccgcgtcg ggtgccaccc tctcggcggt attctctggc tcaagcggga ggaggcgagg
     2881 gccggaggag cgcaaatagg cgtgcacagc gttctgtgcg ataccggacg aatgacgccc
     2941 ggcctcgatg aatatgaacg gcgtcatatc agcgtgcgtc ctgtcgacgt cgaggattac
     3001 gcttcggtag cccttacttt cagcgatggg acgaaagcgc tcgtcatcgc cggcgatacc
     3061 atgctggggg gaacgcgcaa ttacgtagag gtgtacgcca acgacgccgt catgcactgc
     3121 cggattacgc cgtccgatgt tatgagcact tatatgcttg acgaagaggg gttggaaaac
     3181 gtctatatct ccgagatgct ccccgcaaag acgggctggc agcaaccttt tgttgccgag
     3241 gagcaccttc gggggtatgc ggcgcagttc caggacttca tcgagtgcgc agctacggga
     3301 agggctccac tttcggattt tgaactggcc tgcgaaacga tccgcgtcat ttatgcggcg
     3361 tatctctctg ccgaggaggg gcgtcgcatt ctgctgtaga cgccattcgg aggcgaccgt
     3421 aaactcctga cagagcattt cacagtgaaa gcgagtcgcc ttttcgtctg cgtctcgcct
     3481 tcactggatt ggtagggata tgcggtcttt ctgcggcgtg cggctgctct cactgcttcg
     3541 cggcgccgtc ccgagggatg gggcggcagc acggacccgc tttcagcagc gcgctgtccg
     3601 tgtcgtgtcc aacccacccg cggagttccc tcgcaaggtc gagtagtttg tcgaggtcga
     3661 tccccgtgtc gacgcccatc tcatgaagca tgtgaatcat gtcctcggaa gcgacgttcc
     3721 cggaagcgcc gggggcgaag gggcaaccgc cgaggcccgc aagcgaagcg tcgtaacgta
     3781 cgatcccggc ctggagaccg gcaagcgtat ttgcaagggc cattccgcgc gtattgtgga
     3841 agtgaaggaa ccatcggacg tcggggagca tctcctgcat agaggttgcg agatcgtaaa
     3901 cctgccgcgg gttcgccatg ccggtggtat ccgaaaggga gagttcggtg atgccgaggt
     3961 cgtgcagacg ttcagctatt tccgcaacct tttcgggagg taccttccct tcaaaggggc
     4021 agccgaaggc ggtggagatg gctcccgaaa cttcggctcc gttcgaacgg gcaaactccg
     4081 tgcagctttt gaacccgtcg atcagttcgg acggtgtttt gttgaggttc gccttcgcat
     4141 gcgattcgct ggccgacacg gtgagtttca ccttgttcac tcccgaagcg agggcgcgtt
     4201 ccactccctt gagattggcg acaagcgctc ggtactccac accttccatc cggcggatgg
     4261 cgcgtgctac ctcgtcggta tcggccatct gcggaaccgc ctttggattg acgtacgagc
     4321 ctatttccac gatcctgacc ccggcgtcga cgacgccctc gatcaagcgg actttccgct
     4381 cgacggagag cgtggccggc tcgttctgaa gaccgtcgcg ggggccgact tcacaaaaga
     4441 aaatgctttt gggcagtgtc atggtcattg ctatccctcc tttgcatact gatgataaac
     4501 ctctctccgg gacaacagta ctcaaaaaac gaattcctgt caaacggttt atatttccgt
     4561 tcaatgggaa ttcgaagtga tggagaagcg ccgatacaac gaaggtgttt tccgggtgac
     4621 agaaaaaaag aagaggaagt attgacaaag gcgacggcga tagattattg tgtactcatt
     4681 tctttgagat ggtttttgtt tccgttctac gggattttct actctcaatc gtacggggaa
     4741 tccagcatca taaaaacggt tgattgcgaa cttttcaacg gaggtgcgga agaatatgcg
     4801 taaagacctg gtagcgtttc agctcgtgaa gtttctggag gcccgggggg tggagaaggt
     4861 cttcggtctc tgcgggcaca ctgtcatcgg ttttctggac gctctgaaag agagcaaaat
     4921 tacgttcatt acggtacgcc atgaacagat cgccgcgcat gcggccgacg gctatgcgcg
     4981 cgggaagggg tgccgggttc cgggcgttct gctgacgcac ctggggccgg ggctggccaa
     5041 cgcgacgacc ggcgtcgcgg aagcggggct caactctatc cctatggtcg tcatcgcagg
     5101 cgacgtcccc agttgctatt tcggccgtca tcctcatcag gaggtcaacc tccatgcgga
     5161 tgccagccag tacgagatct acaggccgtt tgtgaagcgc gcctggaggg tggacagacc
     5221 ggagctgctt cccgaggtga tggacaaggc gttccgtctc gccgtcacag gacgtcccgg
     5281 accggttctc gtctccgtgc cgatggatat cttctcgatg gagatcgata cccgcttctt
     5341 cgagcagcgg atgcacaata tgccgcttct ccccaggccg gggctcgacg tgaagacagc
     5401 ggaggatatc gccgcgctcc tcgccgaggc gaagagtccg gtgctctacc cggggggcgg
     5461 cgttatctct tctggtgcgg cgtccgcgtt gacggagctc gcaacatttc ttgagatacc
     5521 ggtgctctat acgctcatgg gcaaaggctc cattccggac gatcatccgc tggccgtagg
     5581 catgaccggt ttctggggga cggagttcaa caatagcgcc gcgatgaagg cggacgtgat
     5641 gatggcggtc ggtacgaggc tttcggaggc ggattgcagc tcgtggtact tcggcgagac
     5701 cttcgacgtg cccccgacga agcttattca tatcgacatc aaccaggagg agatcggccg
     5761 taactacact acggagatcg gcgcggtgtg cgacgcgaag atggccctcg acgctatcct
     5821 tgccgctgcg aagcggaagt atccgaacgg cgtgaaacgc ccggagttaa tcggagaaat
     5881 cgccgaagcc aaggccgcgt accgtgcgac gatcgcagag gcgcagaagt cggatcagtt
     5941 cccgatgcgg ccggagcgca tcctggcgga tcttcgggaa gcgcttcccc gagacggctt
     6001 cgtggtggcg gacgtcggct ggaataagaa cggcgtcggg cagcagttcg atatttacga
     6061 accgggaacc tttgtcgcac ccggcggttt ggcgactatg gggtacggtc cctcggcggc
     6121 gctcggcgta aaggtcgcct gccctgacag aaaggtcgtc gcgctcatcg gcgacggggg
     6181 tatgggagcg aacgtctcgc cgtttgcaac cgcggcggtg gagaatatcg gcgttgtctg
     6241 ggttgtgatg aacaactgcg ccttcggtac gatcgccggc cttgagagac agcactacga
     6301 tcatgagttc ggcacgctct tcgagcgcga aggcaagccg tacagccctg atttcgccgc
     6361 cattgcgagg gcgtacggca tcgacggata cacggtcgag cgggcggagg atttcaagcc
     6421 gatgctggaa aaggcactcg cgtccgataa accctgcgtc ctcgacgtcc ggatggaaaa
     6481 cgctcccgtg gtgacgaagg ggtgctggaa catcaacgat atttttagaa agcgcggcga
     6541 ggagaaaccg tggagggtat ggtcctggga ggagatcgag ccgtggagga tgtagaccgg
     6601 caagcgacca cggcaaattt cgagctgtac gccggactga acgcggcgaa tggatgaagg
     6661 agatgacgat gctatggcga aaaaagaact gaccgaagcg cagcgacaga ctctggagac
     6721 ggtcttcgcg agagcgcgcg cggcggagaa gatcatagaa aactacgacc aggagcgtgt
     6781 ggacaggatg tgtcgctgca ttgcctgggc ggcggccaat cctcgcacct tcgaccgatt
     6841 ctgcaagatg ggtgtggagg agagcggcgc gggagattgg agcggccgct acggaaagag
     6901 gcacaagatc ctcggggtgc tgcgcgacgc attgcgccag aagagcgtcg ggatcgtcga
     6961 agtcaacccg gagaaaggac tggtccgtta cggaaagccc gcgggcctga tcacatccct
     7021 gattccgatg acgaatcctg aattgacgcc catcgtcacg gcgatttacg cgctgaaagc
     7081 ccgggatgtg gtcgtcttct ctccgcaccc cagaaccaga aagacgacgt tcgaggttgt
     7141 cgagagcatg cgccaaggtc tgaagtcgat cggcgaaccc gaggattttc tccagtgcat
     7201 caccgaggtc aatatgacta ttgtggagga gctcatgaag atgggcgacc ttgtcatggc
     7261 gacgggaggg ctcgcgatga gcagggccgc ccatagctcc ggaaagcccg cctactgctc
     7321 gggggccggg aatgcgacga tgatcttcga tgaaacagcc gacgttgaag aagcggcacg
     7381 gaacacgcgc atcagcaaga cgtccgactt cggttccggc tgttctgcgg acggcaacct
     7441 ggtcatccac ggctccatct acgagaagat ggtggcgcag ctcgtcaagg agggtggcta
     7501 tctcgccaac gacgaggagc gggagaaact gaagaaggcg atgtgggacg acgaaggaca
     7561 ccggattgta gagacggttg cggtcgctcc tcagaagctc tgcaaggccg ccgggttcga
     7621 gatacccgag gacaggaagt tcgtcatggt cgtaggggag gggatcggca aggagttccc
     7681 cttctccggg gagaagctca cgacgctgct tgcgctctac aggtacgagg gggagttcga
     7741 gaatgcgctg aagatgatag gcgagatcta caaggtcggc gggcggggac actcctgcgg
     7801 tatttacagc ttcaacgagg agcacatcca cgcgctggct ctcagagcgc ccgtcacccg
     7861 tatcatggtc cggcagccgc agtcgaaggc gaacgcggga agcgccaaca acggaatgcc
     7921 catgacgtcg agcatggggt gcggcacctg gggcggaaac caggtttcgg agaacattgc
     7981 actgaaacac tacatgaaca gcacatgggt tgcgaagccg attttgacgg atgctccctc
     8041 tgaagaggtg ctcttcggcg agttctacga tccttcgaac acacgggagg ggtagcttct
     8101 ttccacagcg cgaacgcttc ggaaagcaga cccgtttttt cggattcgtg aatgtaccgc
     8161 tcgacacagt ctttctgatg atggaaaacg tcggcgtggt tttcgtcgga aaggattccc
     8221 ccgacacggc ctaatgacga atgccgtcgt tcgagaacgt ttttttacat tcggaggagg
     8281 agagtacgga ttatgcagcc tcaattttcc ctggcgcagc ttacggtgct gaagacgtcg
     8341 cc
//
LOCUS       DAOTIL010000018         8152 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminobacteriaceae bacterium isolate AMR_MDS_3026
            NODE_10609_length_8152_cov_3.768927, whole genome shotgun sequence.
ACCESSION   DAOTIL010000018 DAOTIL010000000
VERSION     DAOTIL010000018.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868050
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminobacteriaceae bacterium (wastewater metagenome)
  ORGANISM  Aminobacteriaceae bacterium
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae.
REFERENCE   1  (bases 1 to 8152)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8152)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:19:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,224
            CDSs (total)                      :: 3,198
            Genes (coding)                    :: 3,184
            CDSs (with protein)               :: 3,184
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8152
                     /organism="Aminobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10609_length_8152_cov_3.768927"
                     /isolate="AMR_MDS_3026"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:3034609"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..680)
                     /locus_tag="P5201_00735"
     CDS             complement(<1..680)
                     /locus_tag="P5201_00735"
                     /EC_number="3.4.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013047586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016805 - dipeptidase activity [Evidence
                     IEA];
                     GO_function: GO:0070004 - cysteine-type exopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="C69 family dipeptidase"
                     /protein_id="HRV97088.1"
                     /translation="MKLFFSRIAVLLLLLVISSDPSYGCTGILVGREASEDGSVLNSQ
                     TADGWYDSNLRVVPGETFPEGEDVSVYFGLLGDEPLPPFELGRIPQASTTYAYFRTAY
                     SCFNEHQLSIGESTIGQKDMLKAFPGEGGALLTVEQLMILALQRCKTSREAVLLIGEL
                     AERYGFLGSCANEGESLSIADPYEAWIMELLSVGFDWKPGSAPGAIWVARRVPDSHVA
                     ILCNASRIS"
     gene            complement(788..2521)
                     /locus_tag="P5201_00740"
     CDS             complement(788..2521)
                     /locus_tag="P5201_00740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006582825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PEP/pyruvate-binding domain-containing protein"
                     /protein_id="HRV97089.1"
                     /translation="MIYSDFDPKPTFRQFKRLIGDGEVGGKARGIAFAFNAVTGSPLE
                     SVVDFPDVNFVVTTEGFDEFVSDNGIETLLREHAASEEENCDEDTSQELFDRLSAVFQ
                     SGRIRSSLLQDLEGALESIGDFPLAIRSSSILEDSRKLSFAGKYGTRFSANIGSVEDR
                     RDLLANALKDVWASLYNPAARAYRKKHGLVDSDESMAVVIQPVIGRRHNGMYYPEIAG
                     TAFSKVYRRPSTRIKKENGVMRFCFGLGTRTVDRLKANISYLSHPMLRPQGNLPADIA
                     MTSQSEFDYIDRTSGTFMTGLLSEHISFILKEHKLASSFIEIFSDNVLYWAGSDQLSN
                     GKPIFSFSNFPRRHPKFFNIVKDLSAFLEEKMGMPADMEFTYDTESEKLTLLQLRPLA
                     SYEEMARVEIPEVREEKIILMGNKMVSNGKMQNIHHLVFVDPELYGKDATFHEVAREI
                     GRINHKLSGTDYILVGPGRWGSTNPKLGVPVRYNEICNCGCLVEVGILESDYTPELSY
                     GTHFFLDLDVDGTLYLPVFDGMKGNLFNRTWLDSSFYERKRHPAVRHYTGDFSVFMDG
                     EKEIGAVISND"
     gene            complement(2559..3860)
                     /gene="asnS"
                     /locus_tag="P5201_00745"
     CDS             complement(2559..3860)
                     /gene="asnS"
                     /locus_tag="P5201_00745"
                     /EC_number="6.1.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013047784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004816 - asparagine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006421 - asparaginyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine--tRNA ligase"
                     /protein_id="HRV97090.1"
                     /translation="MTALWVSIKDLPSHVEETVVIRGWLYNLRSSGKIHFLQLRDGSG
                     TVQGVMVKKEVSEDLFAAAKGLWLEASVEVTGTVRKDERAPSGVELSVTDLRVFQNPS
                     EEYPIGKKDHGIDFLLDHRHLWLRSSRQQAIMRIRDRVIRSAREYLQENGFLLIDSPI
                     ISGAAGEGVSGLFEIDYFDSKAYLAQTGQLYAEAAAAAFGKVYCFGPTFRAEKSKTRR
                     HLTEFWMLEPEVAFYDHKDNMALQEGLICHIVAKVLEHCRTELASLERDVSKLENIKA
                     PFYHLQYDEAIEMLHKLGSDTPYGDDLGNEDETILTQQFDRPVFIECYPKTVKAFYMK
                     EHPDNPNLVLCDDLLAPEGYGEIIGGSQREDDYDRLIARIREQGLPEEPYEWYLDLRK
                     FGTFVHSGFGMGIERVVAWISGLQHIREAIPWPRTIYRLKP"
     gene            3956..5335
                     /locus_tag="P5201_00750"
     CDS             3956..5335
                     /locus_tag="P5201_00750"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRV97091.1"
                     /translation="MNIPRLVLAEERREGKVAQSILVASVLKSLGYPLRLFCAGVDEH
                     LVRFLKLVTEQDVTVLDPFSCGSMRNFKLLFQRAASPDTLNIIFGSIGERRDENTFPV
                     HPLPGEIARILECPLVTMLYADSSAAVTSRILENVFNQISAQGVITSAVIFSSVLNPR
                     EYQLLETEAGRRVPLLCLGFIPKTLEKNNPPLMELCLQDMAQRAAFPVKAATAQLLGM
                     VDQVDWNVLWGIAKQCKECVAMDDPFKGKASGMSVGILSFPSFELEGNNAALLFKHLG
                     CTVYPIPLDSALLRRDLNALYVPHGPGFLCAERLLGKSGVREWFTSIFHARKAVLVNG
                     GITPLLGESFSLPNRKEYEGLKFFRFKGKFDIPSSDDVRRVEAVSTDGDVMLHQGDKI
                     RGYLPPYISVLNPGNVAGAVWSLQDTVKNMEIGLSGWSRGAALATDVCVELWSSIEGV
                     YRWLMMRKQ"
     gene            complement(5342..6202)
                     /locus_tag="P5201_00755"
     CDS             complement(5342..6202)
                     /locus_tag="P5201_00755"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRV97092.1"
                     /translation="MERPRCSTTHDIIVFDVDGVLVDTSRSYFEVIVQALDWAWTSLI
                     NGVVDCEGFSERHFATTKTHPAFNDDYDIAWAIINCAATAQNPSLAQSLPAPEEWRLL
                     LDGCKESDVPSWVRSTFGEQIPRDTVRRACEELYFGVTKNEKHTALLSPRKGLWENER
                     PMITIPWSAIPVPAGIYTGRTAKELELALKRLHWEDFPDSMIITSDSGVTKPSPRGLE
                     ILCHTSHASTPLFLGDTESDRQTVHAFGRGTFAAIGNFLSDAEFAFPCPEEALEYFGF
                     LSPSNSSVNL"
     gene            6283..7641
                     /locus_tag="P5201_00760"
     CDS             6283..7641
                     /locus_tag="P5201_00760"
                     /inference="COORDINATES: protein motif:HMM:NF012382.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HRV97093.1"
                     /translation="MLLEIPLNYSEGVLYRQLAEHLEKMIRTGSISSGERLPGTRELA
                     RMLRVSRCTVVAAYDVLEENGLVKRTARSGTFAASPLLEGRSSYKGKGDILRFDTECP
                     TRDLLPLRELSELCRGLSLSLLGDALEGSPTEGLPSLRRLLLHHAVLRGIPAHPEEVV
                     VTAGGKDALSTALRSFREHGCSKLWAEELSYSEISSIAENERIPLRTLPLLDESTLSC
                     LDALSSKDVLYLVPSFQNPTGRTLSHPLRQAILSVRRKKGFFILEDDSYGELRYGEKS
                     IPALKAMGEGEGVVYVGSFSQALFPGMRLGYTLLPPFLKEEYLRISSLRQGYVSSLVQ
                     LVVARFLEDGELAAAVERARTILSVRMETLYSLLKSHFSTVPLRKPEGGVFLWFPTGK
                     TDGSEAALLAERHGVRVAPGSRFSLRKRKIAAVRFSITGVPDELLNEAVARLKKAWGK
                     IL"
     gene            complement(7656..7877)
                     /locus_tag="P5201_00765"
     CDS             complement(7656..7877)
                     /locus_tag="P5201_00765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005659423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRV97094.1"
                     /translation="MLVLSMSAVVFFGYAVAGLAGFLLGRGRSDYIIFGLLLGFASTA
                     ASLLLWRHWLSLLEKEALHTADNTGEKLS"
BASE COUNT         1922 a         2046 c         2182 g         2002 t
ORIGIN      
        1 gagatacggc ttgcgttgca cagaatagcc acatgagaat ccggcactct tctggcaacc
       61 cagattgcgc cgggagcgga gcctggtttc cagtcaaatc cgacgctcag cagctccatg
      121 atccacgctt catacggatc cgcgatggaa agcgactcgc cttcattggc gcatgaaccg
      181 agaaaaccgt atcgctcggc aagctccccg atgaggagaa cggcttcacg ggatgtttta
      241 caccgctgaa gggccagaat catgagctgt tcgacggtga gaagagcccc tccttctccc
      301 ggaaaggctt tgagcatgtc cttctggcca atggtggatt ctcctataga gagctggtgc
      361 tcattgaaac atgagtaggc ggtcctgaaa taagcgtagg ttgtagatgc ctggggtata
      421 cgccccagtt caaagggggg aagaggctcg tcgccgagca gtccgaagta gactgataca
      481 tcctcccctt ccgggaaggt ttcccccgga acaacccgaa gattggaatc gtaccatccg
      541 tctgcggttt gtgagttgag aacggaaccg tcttcgcttg cttcgcgccc gacgaggatg
      601 ccagtgcatc cgtaggaagg atccgaactg atgaccaaaa gtaggagcaa tacggcgatt
      661 ctggaaaaaa aaagtttcat ttggaagcga ctcctctaca tagttcgtca gtgcagtcat
      721 tgggagtaga agaagagata cggaatggcc ggccgttccg cagaaaatgg cgaaacggcc
      781 gggtgggtta atcgtttgaa ataactgcgc cgatctcttt ttctccgtcc atgaagacgg
      841 agaagtcccc tgtgtagtgc cgcaccgcag gatggcgttt gcgttcgtag aacgaagaat
      901 ccagccaggt ccggttgaaa agatttcctt tcataccatc aaatactgga aggtagagcg
      961 ttccgtcgac atcaagatcc aggaaaaaat gcgttccgta ggaaagttcc ggggtgtaat
     1021 cgctctccag gataccgacc tccacaaggc aaccgcagtt gcagatttcg ttatagcgta
     1081 ccgggacgcc aagtttgggg ttggtgcttc cccatcttcc gggcccgaca agaatgtagt
     1141 cggttccgga gagcttgtga ttgatgcgtc cgatctctct cgccacttcg tggaaagtgg
     1201 cgtctttgcc gtacaattca ggatccacaa agacgagatg gtgaatattt tgcattttcc
     1261 cgttgctcac cattttgttc cccataagaa taatcttttc ttcgcggact tcgggtatct
     1321 ccacacgagc catctcttcg taagaggcaa ggggccggag ctggagaagc gtcagctttt
     1381 cgctttccgt atcgtaggtg aattccatgt cggcgggcat gcccatcttt tcttcgagga
     1441 aggcggaaag gtccttcacg atattgaaga acttcgggtg ccttcttgga aaatttgaaa
     1501 aagagaaaat aggctttccg ttgctcaact ggtccgaacc tgcccagtag agtacgttgt
     1561 cggagaagat ctcgatgaag gaagaagcga gcttatgttc tttcaggatg aaagaaatat
     1621 gctcagagag aagaccggtc ataaaggttc cggacgttct gtcgatgtaa tcgaactcgc
     1681 tctgacttgt catcgcaata tccgccggca ggtttccctg cggccggagc atcgggtggg
     1741 agagatagga gatatttgcc ttcaggcggt ccactgtccg cgtcccgaga ccgaagcaga
     1801 atcgcatgac cccgttttcc tttttgatcc tcgttgaagg acgccggtac actttggaaa
     1861 aggctgttcc ggcaatttca ggatagtaca tcccattatg ccggcgtcct atcacaggct
     1921 gaataacgac agccatggat tcgtcggagt ctacgagtcc gtgtttcttt ctgtaggccc
     1981 gcgcggcagg attgtacagt gaagcccaaa catccttgag cgcattggcg agaaggtctc
     2041 tgcgatcttc cacgctgccg atgttcgccg aaaaacgtgt gccgtacttt ccggcgaagg
     2101 agagcttgcg cgagtcctcc agaatggagc ttgagcggat cgcgagtggg aaatccccga
     2161 tcgattcaag cgcgccttca agatcctgca acagggaaga acgtattctg ccggactgga
     2221 aaacggcgga aagtctgtcg aaaagctcct gggatgtgtc ttcgtcgcag ttctcctctt
     2281 ctgaagcagc atgttcgcgc aaaagggttt ctattccgtt atcgctgaca aactcgtcga
     2341 aaccttccgt tgtcacgacg aaattcacat ccggaaaatc cacgacagac tctaaagggc
     2401 ttccggtaac agcgttgaaa gcgaaggcga ttcctctcgc ctttcctcct acttcgccgt
     2461 cgccgatcag ccgtttgaac tggcggaacg ttggttttgg gtcaaagtcc gagtaaatca
     2521 cctgagtatg cctccccgaa atgggaaaat aacgaacgtc agggcttcaa acggtaaatg
     2581 gtgcgaggcc atggaatagc ctcacggatg tgctgcaatc cgctaatcca cgccacgacg
     2641 cgctctattc ccattccgaa tccgctgtga acgaacgtgc cgaatttcct gaggtcaaga
     2701 taccattcgt agggttcttc aggaagccct tgctccctga ttcttgcgat aagtctatca
     2761 taatcgtcct ctctttgaga accgccgatg atctcgccgt acccctcggg ggcaagaagg
     2821 tcgtcgcaga ggacaaggtt cgggttgtcg gggtgctctt tcatatagaa tgccttcaca
     2881 gtctttggat agcattctat aaagacgggt ctgtcgaact gctgggtgag tatcgtctcg
     2941 tcttcgttgc cgaggtcgtc gccgtaaggg gtgtcgctcc cgagtttgtg cagcatttct
     3001 atggcttcgt cgtattgaag atgatagaac ggcgccttta tattctcaag cttggagacg
     3061 tcgcgttcaa gcgaggcgag ctccgttctg caatgttcga gtacctttgc gacaatgtga
     3121 catatcagcc cttcctgaag cgccatgttg tctttgtggt catagaaggc gacttccggt
     3181 tcgagcatcc agaactctgt gagatggcgg cgcgtctttg acttttccgc gcggaacgta
     3241 gggccgaagc aataaacctt tccgaacgcc gccgccgcgg cctcggcgta gagctgtccg
     3301 gtttgggcca ggtaggcctt cgagtcgaag tagtcgatct cgaaaaggcc ggaaactcct
     3361 tctccggcgg cgccgctgat gatcgggctg tcaatcagca aaaagccgtt ttcctggagg
     3421 tattcccttg ccgaacggat aacacggtct ctaatccgca tgatcgcctg ctgacggctg
     3481 cttctgagcc agaggtgacg atggtcgaga aggaaatcga taccgtggtc cttctttccg
     3541 atggggtatt cctcagaagg attctggaaa acacgcagat ccgtcaccga aagttcaacg
     3601 ccggaagggg cgcgttcatc ttttcgaaca gtgcccgtaa cctcaacgga tgcctccagc
     3661 cacaacccct tagccgcggc aaagagatct tcggaaactt ctttttttac catgactccc
     3721 tgtactgtgc ccgatccgtc gcgtagctga agaaagtgaa tcttccctga actcctcagg
     3781 ttgtaaagcc atccgcggat aacgacagtc tcctccacat gagaaggaag atctttgatg
     3841 gaaacccata atgctgtcat gcgtcatcaa cctccagacg tcttgaacga taagtgatta
     3901 tcaaattcca tgcggtatat gatacactat actttaaaaa aaaggagagg aaccagtgaa
     3961 tattccacgt cttgtcctcg cggaagaaag aagagagggt aaagttgccc agagtattct
     4021 cgtcgcgtca gtcctgaaaa gtctcggata ccctcttcgt ttgttttgcg caggagttga
     4081 cgagcatctt gttcgttttt taaaacttgt caccgagcaa gacgtaacgg tactcgatcc
     4141 tttttcatgc ggaagcatgc ggaattttaa actcctcttc caacgcgctg cgtcgccgga
     4201 taccttgaac atcatcttcg gttcaatcgg ggaacggcgg gatgaaaaca cctttcctgt
     4261 acaccctctc cccggcgaga ttgcacgtat ccttgaatgc ccgctggtga cgatgctcta
     4321 tgcggatagt tcagccgcag tcacctcaag aattctcgaa aatgttttca accagatatc
     4381 agcacaggga gtgattactt ccgcagtgat cttctcctct gtcttgaatc ctcgggaata
     4441 tcagctgttg gagaccgaag cgggacgtcg cgttcctcta ctttgcctgg ggtttattcc
     4501 gaaaacgctt gaaaagaaca acccgccgct catggaactc tgtcttcagg atatggccca
     4561 gcgagcggcg ttccctgtaa aagccgcgac tgcgcaactg ctgggtatgg tcgaccaggt
     4621 tgactggaat gtcctatggg ggatagcaaa acagtgtaaa gagtgcgttg ctatggatga
     4681 tcctttcaag ggaaaagcca gcggcatgag cgttggaatt ctttcttttc cctcgtttga
     4741 gctggaagga aataacgctg cgttgctttt caagcacctc gggtgcacgg tctacccgat
     4801 tccccttgac agtgcgcttc ttcgccggga cttgaacgca ctttacgttc cgcacggccc
     4861 ggggttcctt tgtgctgaac gactattggg gaagtccggg gttcgggaat ggtttacatc
     4921 gatttttcac gcccggaagg cagtgctggt taatggcggc attacgccgc ttcttgggga
     4981 gtcgttttct ttaccgaaca gaaaagagta cgaggggctc aagttcttcc gttttaaggg
     5041 aaagttcgat atcccttcat ccgatgacgt ccgcagggtt gaggctgttt ctaccgacgg
     5101 agatgttatg ctccatcaag gagataaaat ccgggggtac ctccccccct acatatctgt
     5161 gctgaatccg gggaatgtgg ccggagcagt gtggagtctc caggacacgg tcaaaaatat
     5221 ggagatcggg ctctcgggat ggagcagggg agccgcttta gctaccgacg tgtgcgtcga
     5281 gctgtggagc agtattgaag gtgtgtatcg gtggctgatg atgcggaaac agtgacgtcc
     5341 ttcataagtt tacactgctg ttggatggag agagaaaacc gaaatattcc aaggcctcct
     5401 cagggcaggg aaatgcaaac tccgcatcgg agaggaaatt cccgatggcg gcaaatgttc
     5461 ctctgccgaa agcgtgcacg gtctgccggt cgctttccgt atctccaaga aagagcggcg
     5521 tcgatgcgtg agaggtatgg cacagaatct caagtccgcg aggagatggt tttgtaactc
     5581 cggagtcgga agtgattatc atggaatccg gaaaatcctc ccagtgaaga cgcttgagtg
     5641 caagttccag ctctttggcg gttcgtcctg tataaattcc tgcagggaca ggaatcgcgc
     5701 tccacggaat cgtgatcatc ggacgctcgt tttcccatag tcctttacgg ggcgagagca
     5761 acgcagtgtg cttttcgttt ttcgtgacgc cgaaataaag ttcttcgcat gctctgcgta
     5821 cagtatccct gggaatttgt tccccgaacg tactccgtac ccaggaaggc acgtcagatt
     5881 ccttgcatcc gtcgagcaag agtctccatt cttcaggcgc ggggagactt tgtgcaagag
     5941 agggattctg cgccgttgca gcgcagttga tgattgccca ggcaatatcg tagtcgtcat
     6001 tgaacgccgg gtgtgttttg gtggttgcga agtgacgttc agaaaatcct tcgcagtcca
     6061 cgaccccatt tataagcgac gtccatgccc agtcgagagc ctggacgatc acctcgaaat
     6121 aggaccggga tgtgtcgaca agcacgccgt cgacatcaaa aactataata tcgtgtgtcg
     6181 ttgaacatct tgggcgttcc atcgcaaacg ctccttttca agtatttttc tcccgaagta
     6241 ttgtaccaga tgaaccggcg cagatggaaa ggagagggtg ttttgctgct ggaaataccg
     6301 ttgaactact ctgaaggagt actctaccgt cagctcgccg aacatttgga gaagatgata
     6361 cgaaccggaa gcatctcttc cggcgagcgt cttccgggga cacgggagct tgccaggatg
     6421 ctccgggtaa gccgttgtac tgtcgttgcc gcctacgatg tcctcgaaga gaacggtctg
     6481 gtgaagagaa cggcgagaag cgggactttt gctgcttctc ctcttcttga agggcggtct
     6541 tcttataaag gcaaagggga tattctccgt ttcgatacgg aatgtcccac ccgagatctg
     6601 ctccctctca gagagctttc agaactctgc cgcgggcttt ctctctcctt gctgggcgac
     6661 gcacttgaag ggagtccgac cgagggattg ccttcattga gacgccttct tctgcaccat
     6721 gcagttctgc gaggcattcc tgcgcatccg gaggaagtgg ttgtcaccgc aggcgggaag
     6781 gacgctcttt ccactgctct tcgttctttt cgtgagcacg ggtgctccaa gctatgggcg
     6841 gaagagctgt cctacagtga aatcagctca atagctgaaa atgaaaggat tccgttgcgc
     6901 acacttccgc ttcttgatga gagtactctc tcttgccttg acgcactctc ttcaaaagac
     6961 gtactgtatc ttgtgccgag ctttcagaat cccacgggcc ggacattgtc gcatcctctg
     7021 cgccaggcga tactctctgt caggagaaaa aaaggttttt tcattcttga ggacgacagt
     7081 tacggagaac tccgatatgg agaaaaatcc atcccggcct taaaggcgat gggggaaggg
     7141 gaaggcgtcg tttatgtagg ctccttcagc caggcgctct tcccgggaat gcggctggga
     7201 tacacgctgc ttcctccttt tctcaaggag gaatatttgc ggataagttc tttgagacag
     7261 ggatacgttt cctctctcgt ccagctggta gtcgcacgtt ttcttgagga tggagagctg
     7321 gcggccgcgg ttgaaagagc cagaacaatt ctttcggtcc gcatggaaac gctttacagt
     7381 cttttaaaga gtcatttcag tacagtgcct ctcaggaaac ctgagggcgg agtgtttctc
     7441 tggttcccta cagggaagac agatggatct gaggctgcat tgcttgccga aagacatggc
     7501 gtacgcgtcg ctcccgggag ccgtttttct ctacgaaaaa ggaaaatagc cgctgtccgt
     7561 ttttctataa ccggcgttcc tgatgaattg ttgaatgaag ctgttgcacg tctgaaaaaa
     7621 gcatggggaa aaattctctg aaactgggca tgccgtcagg agagtttttc tcccgtatta
     7681 tctgcggtat gcagcgcctc tttctcaagg agtgagagcc aatgcctcca aagtagaagc
     7741 gacgctgcgg tagatgcgaa accgaggagc aggccgaata taatatagtc agatcttcct
     7801 cttccaagaa gaaagccggc aagaccagcc accgcgtatc cgaaaaacac tacagcggac
     7861 atggaaagca ccaacaccaa aaatcgcacg ttcgtccccc cttttctttt caaaaaggca
     7921 attatagcat caaaaaaaag cagccgtatc ttgtggtttt gcattgaatg tgataaagtg
     7981 gttcaagata tataatacag actgttatac aatgattgaa atactctcga tcaatttaga
     8041 aaattacagg gaggtccttc catgagagag ccgcgtttgt acaccacgtc tgctgattat
     8101 gcataccagg aacttcgcca tcgtatcatc acaaaacaac taaaaccggg cc
//
LOCUS       DAOTIL010000019         7956 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminobacteriaceae bacterium isolate AMR_MDS_3026
            NODE_10942_length_7956_cov_3.355778, whole genome shotgun sequence.
ACCESSION   DAOTIL010000019 DAOTIL010000000
VERSION     DAOTIL010000019.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868050
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminobacteriaceae bacterium (wastewater metagenome)
  ORGANISM  Aminobacteriaceae bacterium
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae.
REFERENCE   1  (bases 1 to 7956)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7956)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:19:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,224
            CDSs (total)                      :: 3,198
            Genes (coding)                    :: 3,184
            CDSs (with protein)               :: 3,184
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7956
                     /organism="Aminobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10942_length_7956_cov_3.355778"
                     /isolate="AMR_MDS_3026"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:3034609"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1337)
                     /locus_tag="P5201_00770"
     CDS             complement(<1..1337)
                     /locus_tag="P5201_00770"
                     /inference="COORDINATES: protein motif:HMM:NF024627.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="right-handed parallel beta-helix
                     repeat-containing protein"
                     /protein_id="HRV97095.1"
                     /translation="MKRRNRFSHRMFLFLLCLLFLSIGSAEAATWTVTRTLDDGSTGT
                     LSHAVTHAAAGDSIVFAGNVTTVDLTSTLYLKKALTISGPATIRQTGANERVLHVTAD
                     CTLTSLTITGGNFVPGTHIGNYWDGAGIYNYANLTMNNCTVTGNTVGGGVYNVKNLTM
                     TGCTISNNTCAGSRHAGGINNEAKSSSAPVLTMYNCVVEGNSNPDTYGGGVFNGEKLF
                     MYGCTARSNSSRGSGGGIYLNLFSETTLAQGCVVTGNSPDQICGPGQYGSLSIDGSCT
                     VGNTAGNKAVALSGFAQGSDPELRKTTGEPDVVAAEGALNDSGSGLFTSVKNTLHIDL
                     GASGEVVAGLASMSATLYNAFTYENVQLADASGEGELVIEFTASWPERVRYYAAFAEY
                     EGVAAGTLAVKGYVVAERGVQFETKPGQPLPDGVTAPDFYEEGEGLMTWRNVVT"
     gene            complement(1417..1560)
                     /locus_tag="P5201_00775"
     CDS             complement(1417..1560)
                     /locus_tag="P5201_00775"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRV97096.1"
                     /translation="MEKKPLKNEGKIQEKDTKKKKKVDEMEESDLNGVAGGEGMDTGI
                     IKP"
     gene            complement(1772..3472)
                     /locus_tag="P5201_00780"
     CDS             complement(1772..3472)
                     /locus_tag="P5201_00780"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="right-handed parallel beta-helix
                     repeat-containing protein"
                     /protein_id="HRV97097.1"
                     /translation="MADKEMKSAMHTGRSVKLLRYFAVSGAALLMFWSVPAVEAKTWT
                     VTTTADSRMKEGSLRYIVAKANGGDTILFSSGGANLTSEIVLDKSLALVGPATIKQTG
                     WERVFSIPAGTTVTMENLTITGGSADYYGGGIYNEGMLTMTGCTVSDNRSARDGGGVY
                     SGVYDYSTQTRTGTLTLTNCTVRNNAATFSGGGVFNGATMTSNGCAFSGNSAYWGGGL
                     LMESNSHASISEGVIENNAATDGGGLFNKGYVQNIQGTSFKRNNAEGKGGAIYGSNGI
                     LTVTACAISANSAGSGGGGIYNTYIPGGRPIELKSGVLVTNNTPDQVNGYYVSDGSCI
                     IGDAAGSKSVALSGFAQGSAPKPRKTSGESDVDAAEQALKDSGSDLFTLVKKSMNADL
                     GASGEVSAALSGMNAKLYDAFAYENVPLADASGKGELVIEFTASWPERARYYAAFAEC
                     GDGAADALSVKGYVIAERGVQFEVKPGQPLPDGVTPPDFYEEGEGLMTWRNVVADNGP
                     YDHNPAVGVVTFRVCSVRAEAIQQQNGGGGGCSVGGAGSAWVAALLFVPVFALARKTR
                     "
     gene            3799..3999
                     /locus_tag="P5201_00785"
     CDS             3799..3999
                     /locus_tag="P5201_00785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013049270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heavy-metal-associated domain-containing
                     protein"
                     /protein_id="HRV97098.1"
                     /translation="MAKFVFSVPDISCNHCKMRISKALEAAGVKEFEVSVESKKVTAE
                     AQDAAKVQAVIEDAGYDATLEA"
     gene            4110..6266
                     /locus_tag="P5201_00790"
     CDS             4110..6266
                     /locus_tag="P5201_00790"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006300608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0019829 - ATPase-coupled monoatomic cation
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HRV97099.1"
                     /translation="MSDKEQQQFKTSLVVTGMTCATCSRMVERSLGKVEGVVFAAVNL
                     ATETAFVVSDREIPKEELVQAVEKAGYSVSEERAEDLEKNRYLRIKKNLALSWLVTAP
                     LMTLMVFHMAGQHVPLYYPLEFFGGALAIFWAGRDSIKGAWIALSHFHANMDVLVVSG
                     SVAAWATAAFAFAGFPVVSFGAVGAMIMALHITGRYIESRLRDKASKEIRALLSIQAR
                     EARVLDGEGKEIMIPIEAVKEGMTLLVKPGERLPSDGELLDGTTSVDESMITGESLPV
                     VKNAGDGVTGGSLNLTGAVRIRATRIGEDSFLAQMVSLIQEAQGAKVPIQAFADRVTN
                     YFVPGVATLAIISGVFWYFQAGRLAPFLDGAARFLPWVTSLRDPLSLSIFAFITTIVI
                     ACPCALGLATPMALITGTGAASKKGLIIRNAEAIQTARDVTVAVLDKTGTITEGKPTV
                     VETDLSPEALAAVASLEAFSNHPLAKAIAAASDERLPVTDLEEITGEGVRGKVAGTTW
                     FVGRPGDASKYDAHLALGQTVVEVRRRDAVTGFVAVEDPLREDAIEGIRRLSEMGISC
                     VMATGDNEKTARAVAARVGITEVRAGVRPDGKLDLVRNLQAKGGKVLMTGDGMNDAAA
                     LKGADIGVAIGSGTDLAIDSADIVIVKGGISRLADAVAISRKTFTVIRQNLFWAFAYN
                     VIAIPLAMAALLHPVIAEGAMAFSSISVILNSMRVK"
     gene            6408..>7956
                     /locus_tag="P5201_00795"
     CDS             6408..>7956
                     /locus_tag="P5201_00795"
                     /inference="COORDINATES: protein motif:HMM:NF012245.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HRV97100.1"
                     /translation="MKLRSILKENAVDIVLFLAAALLAGGYFLYGTGGSATWYVAGLL
                     LLFILMFFKSIFSCSGPAAVVSEWIGRLSRNESVDLRKSVDVPQGSKLSSVANALNAF
                     VQVMRAHFLRIMQGLHKFTFNFYRLERQLGEFFSAFSAMSAGVKEGISSGQNVSHATE
                     SQYASSEEISATAQGLAQLAAELNNAVASAGDRADAGNRRLREMERTFASVVEETKTL
                     SEEARILSGKVDVIQSVVHTITGIAEQTNLLALNASIEAARAGDAGRGFAVVADEVRK
                     LAEESKTAAATISKNLQELVSGVHTTSGGVETMAAKMNEANENVGAVLSEISALLEGI
                     SGISDSSERVAASAQELGASSEELAASAETVTRETEKMSSIFGDIEGKISSLSSTAEG
                     LNETSKEGSIDAAALIHQLSVLKAMKADDFADIAEDAIKAHKGWVANLKKFVEGGQWD
                     LETNPQRCRFGIFLSFIERPEGASEELWSGILSMHEKLHGLGHTVNDAMQRGESGKAR
                     EVLKETVA"
BASE COUNT         1471 a         2486 c         2412 g         1587 t
ORIGIN      
        1 gtcaccacgt tccgccacgt catcaacccc tcgccctcct cgtagaagtc gggcgccgtt
       61 acgccgtcgg ggagcggctg ccccggcttc gtctcgaact ggacgccgcg ctcggcaacg
      121 acgtacccct tcacggcgag agtaccagcc gccacgccct cgtactcggc gaacgccgca
      181 tagtagcgga cgcgctccgg ccacgaggcg gtgaactcga tgaccagctc tccctcgcca
      241 gacgcgtcgg caagctgcac gttctcatag gtgaaggcgt tatacagcgt cgcgctcatg
      301 ctcgcaaggc cggcgacaac ctcccccgaa gcgccgaggt cgatatgcag cgtattcttc
      361 accgacgtga acaggccgct cccggagtcg ttcagcgcac cctccgcagc gacgacgtca
      421 ggttcgccgg tcgtcttccg gagttcggga tcgcttccct gcgcgaagcc ggagagcgcg
      481 acggccttgt tccccgccgt gtttccgacg gtgcacgaac cgtcgatact caacgagccg
      541 tactgccccg gcccgcagat ctggtcggga gagttccccg tcaccacgca cccctgggcc
      601 agggtcgtct cgctgaagag attgaggtat ataccgcctc ccgaccccct gctgctgttg
      661 ctccgcgccg tgcacccgta catgaagagt ttctccccgt tgaagacgcc gcctccgtag
      721 gtatcggggt tcgagttccc ctccacgacg cagttgtaca tcgtcagtac cggagcgctg
      781 gatgatttag cctcgttgtt gatgccgccc gcgtgccgcg atcctgcgca ggtgttgttg
      841 ctgatcgtgc agccggtcat ggtcagattc ttcacattgt acacgccccc gccgacggtg
      901 ttgcccgtca cggtgcagtt gttcatcgtc aggttcgcgt agttgtatat tcctgcgccg
      961 tcccagtaat ttccgatgtg cgtccccgga acgaagttcc cgccggtgat ggtcagcgaa
     1021 gtgagcgtgc agtcggccgt tacgtggagc acgcgctcgt tcgcccccgt ctggcggatc
     1081 gtcgcagggc cgcttatggt gagcgccttc ttgagataaa gcgtcgaggt cagatcgacc
     1141 gtcgtgacat ttccagcaaa tacgatacta tcgcccgcag cggcgtgcgt caccgcgtgg
     1201 ctcagcgtcc ctgtggaacc gtcgtcgagc gttctggtca ctgtccacgt cgcggcctcc
     1261 gcgctcccga tgctcaagaa gagcaggcag agcagaaaga ggaacattct atgagaaaag
     1321 cggtttcttc gtttcaccgt cgatcaccct ttcgaaaaaa gcttgaacag aaaaggacgg
     1381 agggcgctgt tccaaccctc cgtccgtcca caaagatcaa ggcttgatga tccccgtatc
     1441 cattccttcg ccgccggcga ctccgttgag atcgctctct tccatttcgt cgaccttctt
     1501 ctttttcttc gtatccttct cctggatctt cccttcgttt ttgagcggtt tcttttccat
     1561 cccgaacacc tccatcgttt tgagtacatt caccgcaccg cgcatcgccc gacgtttccg
     1621 tctatagaga gatgataaac cttgacacta tggtaaagtc aaggggtaag catataaatc
     1681 ttctcttttt tctcaaaagt cggtctgtac atccgcacac aggctctgca agcagaggat
     1741 gtctctttga tgagtcgttt ctctcaaggt tctatctcgt tttcctcgcg agcgcgaaga
     1801 ctggaacgaa gagcagggcg gcgacccacg cacttccggc gccgccgaca gagcagcctc
     1861 cccctccgcc gttctgctgt tggatggctt ccgcgcggac ggaacagacg cggaaggtca
     1921 cgacgcccac ggcaggattg tggtcgtacg ggccgttgtc cgccaccacg ttccgccagg
     1981 tcatcagccc ctcgccctcc tcgtagaagt cgggaggcgt tacgccgtcg gggagcggct
     2041 gccccggctt gacctcgaac tgcacgcccc gctcggcgat gacgtacccc ttcacggaga
     2101 gagcgtccgc agccccgtct ccgcactcgg cgaaagccgc gtagtaccgg gcgcgctccg
     2161 gccacgacgc ggtgaactcg atgacaagct ctccttttcc cgacgcatcc gcaagcggca
     2221 cgttctcgta ggcgaaggcg tcgtacaact tcgcgttcat tccggaaaga gcggcggaaa
     2281 cttctcccga tgcgcccaga tcggcgttca tgctcttttt gaccagcgtg aacaggtcgc
     2341 ttccggaatc cttcagcgcc tgctccgccg cgtcgacgtc cgactccccg ctcgtcttcc
     2401 ggggtttggg agcgcttccc tgcgcgaagc cggagagcgc gacggatttg cttcccgcag
     2461 catctcctat gatgcaggag ccgtcgctca cgtagtatcc gttgacctgg tccggcgtat
     2521 tgttcgtcac gaggacaccg cttttgagtt cgatgggtcg cccccccgga atatacgtat
     2581 tgtatatgcc tcccccgccc gaaccggcgc tgttggcaga gatggcgcag gccgtcaccg
     2641 taaggatgcc gttggatccg tagattgcgc ctcctttccc ctccgcgttg tttctcttga
     2701 aggacgttcc ctgtatgttc tgcacatacc ccttgttgaa gaggccgccg ccgtcggtag
     2761 cggcgttgtt ttcgattacg ccctcgctga tcgaggcgtg cgagttgctc tccatcaaaa
     2821 gtccgccgcc ccagtaggcg gagttcccgg aaaacgcgca cccgttcgat gtcatcgttg
     2881 cgccgttgaa cacgccgccc ccggaaaacg tcgcggcatt gttccgcact gtgcagttcg
     2941 tcagggtcaa cgtgcccgtc ctggtctgcg tgctgtagtc atagacgccg ctataaacgc
     3001 ctcctccgtc tcttgcggag cggttgtcgg agaccgtgca gcccgtcatc gtcagcattc
     3061 cttcgttgta tattcctccc ccgtagtaat cggcgctccc tccggtgatc gtcaggttct
     3121 ccatcgtcac cgtcgtcccg gcggggatgg agaagacacg ctcccacccg gtctgtttga
     3181 tcgtcgccgg acctacgagc gcgagactct tgtccaggac gatctccgag gtaaggttgg
     3241 ctccgccgct ggagaagagg atcgtatcgc ctccgttcgc tttcgcgacg atgtagcgaa
     3301 gcgacccctc cttcatcctc gaatcggccg tagtggtcac cgtccacgtt ttcgcctcga
     3361 ctgcgggcac gctccaaaac atcaggagcg ccgctcccga tacagcgaaa taccttaaaa
     3421 gttttaccga cctgcccgta tgcattgccg atttcatctc cttgtccgcc atgacttcgt
     3481 ctcatgaggc ggggtatgtc tttctatcgc agaatcataa taaaccttca caccgtgtaa
     3541 gagtcaaggg ggaaattaaa aaatctgctt agtcttccca aaaaaaacaa aaaaaattat
     3601 gctccgaaag cggacagaat tcccgctccc cttgatcgcc tctacattcg cgttcaagag
     3661 atttttcggt gatccgtaga gagtatctat tttttaattt ctttttcagc cctctggcgt
     3721 aaatacccca agtggtatat actacaaaag cgatagagtt tatcctatcg aacatacgat
     3781 cacaaggagg gcatttctat ggcgaagttt gttttttccg ttcccgacat ctcctgcaac
     3841 cactgcaaga tgcgcatatc caaagccctt gaggcagcgg gagtgaagga gttcgaagtc
     3901 tccgtggagt cgaagaaagt gactgcggag gcgcaggacg cggccaaggt acaggcggtc
     3961 atcgaggacg cggggtacga cgcaacactt gaagcgtagc gccgataagg accggtatac
     4021 gttcgcggac gagaaacgtc ttttccgtaa gggctcacgt ccgcgaacgc ccggttccgg
     4081 acacattttt gtatttggag gaaaaatcca tgagcgataa agagcagcag cagttcaaga
     4141 caagcctcgt cgtgacgggg atgacgtgcg ccacgtgttc gcggatggtc gagcgatcgc
     4201 tcggcaaggt ggagggcgtg gtcttcgcgg cggtgaacct ggccacggag acggcattcg
     4261 tggtgtccga ccgcgaaata cccaaggagg agcttgtaca ggctgtcgag aaggcgggtt
     4321 actcggtctc ggaggagcgc gccgaggacc ttgagaagaa ccgctatctg aggatcaaga
     4381 agaacctcgc gctctcctgg ctggtgacgg ccccgctgat gacgctgatg gtctttcaca
     4441 tggccgggca gcacgttccg ctctactatc cgctggagtt cttcggcgga gcgctcgcca
     4501 tcttctgggc cgggcgcgac tcgatcaagg gggcgtggat cgcgctctcg cacttccacg
     4561 cgaatatgga cgtactggtg gtctcgggct cggtggccgc atgggcaacg gcggccttcg
     4621 ccttcgccgg ctttcctgtg gtgtcgttcg gcgcggtggg cgcgatgatc atggcgctcc
     4681 acatcaccgg acgatacatc gaatcgcgcc tgcgggacaa ggcgtcgaag gagatccgcg
     4741 cgctgctctc catccaggcg cgcgaggcgc gcgtgctcga cggcgagggg aaggagatca
     4801 tgatccccat cgaggccgtc aaggagggga tgacgctgct ggtgaaaccg ggcgaacgcc
     4861 tcccgagcga cggggagctg ctcgacggga cgacttcggt ggacgaatcc atgatcacag
     4921 gagaatcgct ccccgtggtg aagaacgcgg gcgacggcgt gacgggcggc tcgctaaacc
     4981 tcaccggggc cgtacgcatc cgggccaccc gcatcggcga ggacagcttc ctcgcgcaga
     5041 tggtctcgct gatccaggag gcccaggggg cgaaggtgcc gatacaggcc ttcgccgacc
     5101 gcgtgaccaa ctacttcgtg ccgggcgtgg cgacgctcgc gatcatcagc ggcgtcttct
     5161 ggtacttcca ggccggacgg ctcgccccat tcctcgacgg cgcggcgcgt tttctcccgt
     5221 gggtgacctc gctccgcgat ccgctctcgc tctccatctt cgccttcatc accaccatcg
     5281 tcatcgcgtg cccgtgcgcg cttgggctgg caacgccaat ggccctgatc accggcacag
     5341 gcgcggcctc gaagaagggg ctcatcatcc gcaacgccga ggccatccag acggcgcggg
     5401 atgtgacagt agccgtgctg gacaagacgg gcaccatcac cgaaggaaaa ccgaccgtcg
     5461 tggagacgga cctttctccc gaggcgctcg cggccgtggc atcgcttgaa gccttctcga
     5521 accacccgct cgccaaggcg atcgccgccg cgtccgacga acgccttccc gtgaccgacc
     5581 tggaggagat caccggcgag ggcgtgcgcg ggaaggtcgc gggcacgacc tggttcgtcg
     5641 ggcgtcccgg ggacgcctcg aagtacgatg ctcacctcgc gctcgggcaa acggtggtcg
     5701 aggtccggcg ccgcgacgcc gtaacaggct tcgtcgcggt ggaggacccg ctgcgtgagg
     5761 acgccataga aggaatccgc cgcctctcgg agatgggcat ctcctgcgtt atggcgacgg
     5821 gcgacaacga gaagacggcc cgtgccgtgg ccgcacgcgt gggcatcacc gaggtgcggg
     5881 cgggcgtccg ccccgacgga aagctcgacc tggtacggaa cctccaggcg aagggcggca
     5941 aggtgctgat gacgggcgac ggcatgaacg acgccgccgc gctcaagggc gccgacatcg
     6001 gcgtggcgat cggctccggg acggatctcg ccatcgacag cgcggacatc gtcatcgtca
     6061 agggcggcat ctcgcgtctt gccgacgctg tggccatctc gcgcaagacc ttcacggtga
     6121 tccggcagaa tctcttctgg gccttcgcct acaatgtgat cgccatcccg ctcgccatgg
     6181 ccgcgctgct ccaccccgtc atcgccgagg gcgcgatggc gttcagctcc atctcggtga
     6241 tattgaactc gatgcgggtg aagtagcgtg tgtccgcgaa cggagagagg cggtccgaac
     6301 agatgaagcc gccgttccgg tgtgcgggtt tgatgaaaac gctttgtttt cgataagagg
     6361 tatggtatcg tttcatggaa acaatacgat gaaggtgggg tcattcaatg aagctccgaa
     6421 gtatactcaa agaaaacgcg gttgatatcg tcctctttct tgcggcggcg ctccttgcgg
     6481 gcggttattt cctgtacggg acgggcggaa gcgcgacctg gtacgtggcc ggactcctcc
     6541 tgcttttcat tcttatgttt ttcaagagca tcttcagctg ctccggccct gccgctgtcg
     6601 tctccgagtg gatcgggcgg ctttcgcgga acgagtcggt cgatctgcgc aagagcgtcg
     6661 acgtgccaca gggttcgaaa ctgagctccg tggcgaacgc cctcaatgcg ttcgttcagg
     6721 tcatgcgggc gcactttctg cgcatcatgc aggggctgca caagttcaca ttcaacttct
     6781 accgtctgga gcggcagctc ggcgagttct tttcggcctt ctccgcgatg tccgcaggcg
     6841 tgaaggaggg gatctcctcc gggcagaacg tctcgcatgc gacggagtcg cagtacgcct
     6901 cgtccgagga gatttcggcc accgcgcagg ggctggcgca gctggcggcc gaactgaaca
     6961 acgccgtcgc ctccgctggc gacagggcgg acgccgggaa cagacgcctc cgcgagatgg
     7021 agcgcacctt cgcctccgtc gtcgaggaaa cgaagacgct ttcagaggaa gcgcgcatcc
     7081 tctccggcaa ggtggacgtc atccaaagcg tggtgcacac gattacggga atagcggagc
     7141 agacgaatct cctcgcgctc aacgcttcga tcgaggcggc ccgcgccggg gatgccggcc
     7201 gggggttcgc cgtggtcgcc gacgaggtgc gcaagctggc cgaggagagc aagacggccg
     7261 ccgcgacaat ctccaagaac ttgcaggagc tcgtctccgg agtgcacacc acctcgggag
     7321 gcgtcgagac gatggctgcg aagatgaacg aagcaaatga gaacgtcggc gccgtcctgt
     7381 cggagatttc cgcgctgctc gaaggtattt cggggatttc cgactcgtcg gagcgcgtcg
     7441 cggcgagcgc gcaggagctc ggggcgtcct ccgaggagct tgcggcctcc gcggagaccg
     7501 tcacccgcga gacggagaaa atgagctcca tcttcggcga catcgaggga aagatatcct
     7561 ctctctcttc caccgccgaa gggctgaacg aaacttcaaa ggagggctcg atcgacgccg
     7621 ccgccctcat ccaccagctt tccgtcctca aggcgatgaa ggcggacgac ttcgccgaca
     7681 tcgcggagga cgcgatcaag gcgcataagg gctgggtggc caatctgaag aagttcgtgg
     7741 agggcggaca gtgggacttg gagacgaatc cgcagcgctg ccgcttcggc atcttcctct
     7801 ccttcatcga gcggcctgag ggagcttccg aagagctgtg gagcggcatc ctctcgatgc
     7861 acgagaagct gcacggtctg gggcacacag tcaacgacgc gatgcagcgc ggcgagagcg
     7921 gcaaggcgcg ggaggtcctg aaggagaccg tagccc
//
LOCUS       DAOTIL010000020         7716 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminobacteriaceae bacterium isolate AMR_MDS_3026
            NODE_11371_length_7716_cov_4.015142, whole genome shotgun sequence.
ACCESSION   DAOTIL010000020 DAOTIL010000000
VERSION     DAOTIL010000020.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868050
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminobacteriaceae bacterium (wastewater metagenome)
  ORGANISM  Aminobacteriaceae bacterium
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae.
REFERENCE   1  (bases 1 to 7716)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7716)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:19:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,224
            CDSs (total)                      :: 3,198
            Genes (coding)                    :: 3,184
            CDSs (with protein)               :: 3,184
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7716
                     /organism="Aminobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11371_length_7716_cov_4.015142"
                     /isolate="AMR_MDS_3026"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:3034609"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..374
                     /gene="whiA"
                     /locus_tag="P5201_00800"
     CDS             <1..374
                     /gene="whiA"
                     /locus_tag="P5201_00800"
                     /inference="COORDINATES: protein motif:HMM:TIGR00647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0043937 - regulation of sporulation
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="DNA-binding protein WhiA"
                     /protein_id="HRV97101.1"
                     /translation="QEIADILASFNMVKTMLHLEEKAIFRSLRNQANKLVNCDASNIR
                     KSLEASSRQIEISRNALELPDYDDFPAVLKDLISTRLANPSMTLEELGRIQSPPVSKS
                     TIKYRWKKIEDLATPLWQRRK"
     gene            472..1659
                     /locus_tag="P5201_00805"
     CDS             472..1659
                     /locus_tag="P5201_00805"
                     /EC_number="2.7.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012869638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004618 - phosphoglycerate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate kinase"
                     /protein_id="HRV97102.1"
                     /translation="MKLRTFSESDVRGKNVLVRVDFNVPLKNGKVTDSTRILAHRETV
                     STLLGWGAKVALSSHLGRPKGKRVPEMSLECVLSEAEKAFRVPTRFCNDCIGPSVQSA
                     VDGMKAGELLLLENLRFYAEEQENDTAFAGKLAAPFDVFVMDAFSASHRADASTNAIM
                     GILPSFSGALLRREVEMLSAVSEKPESPFVLILGGAKVSDKIGVVEHLMDKTSTILIG
                     GGMAFTFLKAAGKPIGRSLFEAEKKDFALEMMEKAQRSGVEILLPTDVVVGTSLEMTS
                     GEKIVSADALPDDAMGLDIGPKTVSLFAERIAGARTVLWNGPMGVFENPLFASGTKGV
                     AEGVAACTRKGGITVVGGGDTAAAAKMLGFDQNVSHVSTGGGASLEFCEGKDLPGIKP
                     LEE"
     gene            1687..2481
                     /gene="tpiA"
                     /locus_tag="P5201_00810"
     CDS             1687..2481
                     /gene="tpiA"
                     /locus_tag="P5201_00810"
                     /EC_number="5.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014806733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004807 - triose-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="triose-phosphate isomerase"
                     /protein_id="HRV97103.1"
                     /translation="MKKIYLYGNWKMNMLPSEAEAFSNELLSETAGELYSTRSVDIAL
                     FPPYISIAPVVRMLPEKSFIKAGAQDGWFEDRGAYTGAVSMDMIREIGCTHVLVGHSE
                     RRSIFGDTDEIVGRKLKQAIQKGLIPVLCFGETLEQREAGQTLAILGKQLLAAWKDIE
                     ADEIKGSDNLPGILLAYEPVWAIGTGRNAKAADAQEACAFSRNLFKERFGKQMDCPVL
                     YGGSVKESNAAELLSQQDIDGALIGGASLKVGSFLGIYENYRKSAD"
     gene            complement(2615..2974)
                     /gene="rsfS"
                     /locus_tag="P5201_00815"
     CDS             complement(2615..2974)
                     /gene="rsfS"
                     /locus_tag="P5201_00815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013048238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0017148 - negative regulation of
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome silencing factor"
                     /protein_id="HRV97104.1"
                     /translation="MKKQDDRLKEYEFLYEALADKRGLDIVALDLEGSSAISDVFILV
                     TANSDVHMDTLRDAALEALHVQGIKTTVEGNDSMRWRLIDAGDLTVHVFSKAGRDHYN
                     LEKMWRDARSYHYAYHD"
     gene            complement(2995..4278)
                     /locus_tag="P5201_00820"
     CDS             complement(2995..4278)
                     /locus_tag="P5201_00820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013048239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0070726 - cell wall assembly [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LCP family protein"
                     /protein_id="HRV97105.1"
                     /translation="MKILRIVAILLLAMGAAFAGAAFKVKTFVEPQPQTIRESISFTE
                     ESGSINLLLIGIDDVDGGRRADAIAYATIDIDNKIVRLMSIPRDTRVQLPKRGWEKIA
                     HAYAYGGVELLRETVVNYLGMPINYYVLVNYDTFPSIVDLIGGVEVDVPKRMVYNDYS
                     GKLFINIPKGLQVLDGKNALHYVRFRHDALGDIGRVKRQQDFIKAVLAKLQSPYMIPK
                     IPDLANKAIELVNSDMTPAQGLQLASYLVSMPSENIHFFTMPGKAAYISNISYWVGDI
                     TAASALLTGVPEGHGTLSGDVAKEETDMGTLDLSTTDVPALISSINTPVAVLNGAGVS
                     GLGKTMATALQKIGIDVTHVGNARHFDYRTTTIHLPEQTTDPVAETGKTLAELLSVNP
                     RLISRTANVSHATIIIGKDHALVLKRLEELAEKKQ"
     gene            complement(4301..4894)
                     /gene="nadD"
                     /locus_tag="P5201_00825"
                     /pseudo
     CDS             complement(4301..4894)
                     /gene="nadD"
                     /locus_tag="P5201_00825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009163820.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate-nucleotide adenylyltransferase"
     gene            complement(4952..6319)
                     /gene="obgE"
                     /locus_tag="P5201_00830"
     CDS             complement(4952..6319)
                     /gene="obgE"
                     /locus_tag="P5201_00830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005662036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase ObgE"
                     /protein_id="HRV97106.1"
                     /translation="MKFVDFTRISVRGGRGGNGCLSFRREKFIPKGGPDGADGGRGGD
                     IILEAAPGALTLADFEYEKKFQAGNGEPGKGGMKSGGAGNDKIVIVPCGTIVYDDDTG
                     EILADLVEPGDRLIAAKGGRPGKGNAHFANSVRKSPRFAEKGDEGEARTLLLELKLIA
                     DVGLVGVPNAGKSSLLGAISSARPKIAGYPFTTLSPNLGVLSVDDQKIVIADVPGLIE
                     GAHENKGLGVYFLRHVERTRFLVHVLDLSAGTADEVIEQWSIIRNEFEAYSNSEESGL
                     PGYHRQDLLLRPYIVVGNKIDLENAAENDMHIRSFMEEQGIPYFSVSALSGEGIPEFI
                     QLIAEYARENQRHEGETRLAPLVVEIQEPRRWRRKLEPVTIIKLGDGAGFRVVHANLE
                     KTLLRYDFEQEDALPKFARLVKKFRIEERLEEMGAVKGDKVFIGDHEFDFEPDMAGAS
                     RSENR"
     gene            complement(6479..6766)
                     /gene="rpmA"
                     /locus_tag="P5201_00835"
     CDS             complement(6479..6766)
                     /gene="rpmA"
                     /locus_tag="P5201_00835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006301087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L27"
                     /protein_id="HRV97107.1"
                     /translation="MKWNFDLQLFAHKKGQGSSTNGRDSEGKRLGIKIYAGQTVQAGN
                     ILVRQRGTKIHPGKNVGLGKDYTIFATSAGTVRYETKGSRKIVSVVLQAAE"
     gene            complement(6750..7091)
                     /locus_tag="P5201_00840"
     CDS             complement(6750..7091)
                     /locus_tag="P5201_00840"
                     /inference="COORDINATES: protein motif:HMM:NF016233.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal-processing cysteine protease Prp"
                     /protein_id="HRV97108.1"
                     /translation="MTEISVSFDEERPVALVAEGHSGYAERGEDIVCAGVSALLQALL
                     FGFEKVVSSPGFSFSINEDSARMVLDWRRSPPEQTAVLVETIVGSLKEIARVYPEHVR
                     ILEVPVDEMEF"
     gene            complement(7094..7411)
                     /gene="rplU"
                     /locus_tag="P5201_00845"
     CDS             complement(7094..7411)
                     /gene="rplU"
                     /locus_tag="P5201_00845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013048244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L21"
                     /protein_id="HRV97109.1"
                     /translation="MYAIVETGGKQYRVQEGDLLKVESLGLEEGASVVFDKVLLLGRD
                     DAVVVGAPYVKGATVTASVTGNGREPKILVFKFKSKKNYRRMRGHRQNYSEIRIDTLT
                     AGV"
     gene            complement(7520..>7716)
                     /locus_tag="P5201_00850"
     CDS             complement(7520..>7716)
                     /locus_tag="P5201_00850"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRV97110.1"
                     /translation="VKKLERDDRIGEVARMLSGDASLQEAREHAERLLYSAESTECSQ
                     SGNISVLAGEGDLAALRGKV"
BASE COUNT         1914 a         2067 c         1835 g         1900 t
ORIGIN      
        1 agcaggagat tgcagacata cttgcgagtt tcaacatggt aaagacaatg ctccatcttg
       61 aggaaaaggc gatcttccga tcgttgagaa accaggcaaa caagctggtg aactgtgacg
      121 cctcgaatat tcggaagagc ctggaggcat cgtctcgtca gatcgaaata tcgcgcaacg
      181 cactcgaact ccctgattac gatgatttcc cagccgttct gaaagacctt atatccacac
      241 gtctggccaa tccaagtatg acgttggaag aactcggccg tatccagtct ccccctgtaa
      301 gcaaaagtac tataaaatat cgctggaaaa aaatagaaga cctggcaacc cctttatggc
      361 agcggcgaaa ataaaatttc tctaacttca gatcccgcga tgccaaagtg aaggggtacg
      421 ccatataata gaaatgctca cgacaaagaa gctaaaggag gtcggaaaca catgaaactg
      481 cgtacgtttt cagaaagcga tgtccgtggg aaaaatgtgc ttgtgcgggt tgatttcaat
      541 gttccgttga aaaatgggaa agtaacggat tccacgcgaa tccttgccca cagggaaact
      601 gtttctacgc ttctgggatg gggagccaag gttgcgctct cttcccatct tggccgacca
      661 aaagggaaaa gagttcctga aatgtctctc gaatgcgttt tgtcggaagc ggaaaaggcg
      721 ttccgtgttc caaccagatt ttgcaacgac tgcattggac cttcagtcca atctgcagtt
      781 gacggaatga aagcggggga gttgctcctg ctcgaaaacc ttcgttttta cgcggaagaa
      841 caagaaaatg acacggcgtt cgccggcaag ctcgccgctc cctttgatgt cttcgtcatg
      901 gacgcgttca gcgcctcgca cagagcagac gcgtcgacaa acgctattat ggggattctc
      961 ccctcctttt caggcgctct gcttcgtaga gaagtggaaa tgctgtcagc cgtgagcgag
     1021 aagcctgaat ctccgttcgt ccttatcctc ggaggcgcaa aggtttcgga taaaatcggc
     1081 gtggtcgagc atctcatgga taagacaagt acgatactca tcggaggcgg tatggccttc
     1141 acgttcctta aagcagccgg gaaaccgatt ggacggtccc ttttcgaggc ggaaaagaaa
     1201 gactttgccc tcgagatgat ggagaaggca cagcgctcag gagtcgaaat cctcttaccg
     1261 acagacgttg ttgtaggaac ctctctagaa atgacgagcg gtgaaaagat tgtgtctgcc
     1321 gacgctcttc ctgacgatgc catgggattg gatatcggac cgaagaccgt atcccttttc
     1381 gccgaaagaa tcgcaggagc cagaactgtt ctctggaacg ggccgatggg cgtcttcgaa
     1441 aatcctctct ttgcctcggg aacgaagggg gtggctgaag gagtggccgc atgcacccgc
     1501 aaaggtggca ttaccgtcgt cggaggagga gatactgccg cagcggcaaa aatgctcggc
     1561 ttcgaccaga atgtctctca cgtttcaaca ggaggcgggg caagtcttga gttttgcgaa
     1621 gggaaagacc ttccgggaat caaaccgctc gaagagtaaa caataaagga ggccgatgag
     1681 cataccatga aaaaaatcta tctttatggc aactggaaga tgaacatgct tccttccgaa
     1741 gcggaagctt tttcaaacga acttctttct gaaacagcgg gggaactgta tagcacacgt
     1801 tcggtcgaca tcgctctttt cccgccctac atatcgatag cgccagtagt tcgcatgctc
     1861 ccggagaaaa gcttcatcaa agcgggcgct caggacggct ggttcgaaga ccggggcgcc
     1921 tacaccggtg cagtatccat ggatatgatt cgtgaaatag ggtgcacgca tgtgcttgtg
     1981 ggtcatagtg aaagaaggag catattcggt gatacagatg agatcgtcgg gcgaaaactg
     2041 aaacaggcca tccagaaagg acttataccc gtactttgtt tcggagagac gcttgaacag
     2101 cgtgaagctg gacaaacgct cgcaattctt gggaagcagc ttctggcggc atggaaggat
     2161 atcgaagcgg acgaaatcaa aggttcggat aaccttcccg gtattctgct tgcatatgaa
     2221 cctgtatggg ctatcggcac aggacgtaac gcaaaggctg cggatgctca ggaggcatgt
     2281 gctttttcac ggaatctttt caaagagcga tttggtaagc agatggattg tcctgttctg
     2341 tacggaggca gcgtcaaaga atcaaatgcc gcggagcttt tatctcagca ggatatcgac
     2401 ggcgctctta tcggaggagc ttctttgaag gtcggttcgt tccttggaat ttatgaaaac
     2461 taccggaagt cggcggactg aaaaagagaa tatgtaacat aagatacatt ataggacacc
     2521 tcaaaaagat aactgaaaaa ccgggcagac ttcgtttcgg tctgcccggt tctcttttga
     2581 atctctttat agaaacgaaa aaaacgcagc gttctcaatc atggtatgca taatgatacg
     2641 agcgcgcgtc tctccacatc ttctctaggt tatagtgatc tctcccggct ttactgaaca
     2701 catgtaccgt caggtctccg gcgtcaataa gacgccaccg catactgtcg tttccctcga
     2761 cggttgtctt gattccctgc acgtgaagcg cctccagagc tgcatcccgc agcgtgtcca
     2821 tatgtacgtc ggaatttgcc gtcaccaata tgaaaacatc tgaaatcgcc gaagagcctt
     2881 ccaggtcaag cgccacgata tcaagccctc tcttgtccgc cagcgcttcg tacagaaatt
     2941 catactcctt gagccggtca tcctgtttct tcaagctgtc accctcccct acacttactg
     3001 ttttttttca gctaactctt ccaatcgctt caacacaagc gcgtggtcct ttcctatgat
     3061 tattgtagca tgcgaaacat ttgcagttcg tgaaattaga cgagggttca cgctgagaag
     3121 ctccgccagc gtctttccag tctctgcaac cggatcggtt gtttgttccg gaagatgtat
     3181 cgttgttgta cgataatcaa agtgacgcgc attcccaaca tgggtaacat caatcccaat
     3241 cttttgcaat gccgtcgcca ttgtctttcc aagacctgag acgccggcgc catttaaaac
     3301 agccacaggc gtatttattg aagagatcaa tgccggaaca tccgttgtag agagatcaag
     3361 cgttcccata tcggtctctt ctttcgccac atctccggat agcgttccgt gtccttccgg
     3421 aacccccgtt aacagcgcgg aggcggctgt gatgtcgcca acccagtagc tgatattcga
     3481 gatgtacgcg gccttcccgg gcatggtaaa aaaatgtatg ttttcactcg gcatcgaaac
     3541 aaggtaggac gcaagctgta acccctgagc aggcgtcata tccgagttca ccaactcaat
     3601 cgctttattc gccaagtcag gtatcttcgg tatcatgtaa gggctctgca atttcgccaa
     3661 aacagccttt ataaagtcct gctggcgctt gacacgacca atatccccca gcgcgtcatg
     3721 acggaatcga acgtaatgga gtgcattctt cccgtcgagc acctgcaaac cctttgggat
     3781 attgataaaa agtttgcccg aatagtcatt gtagaccatg cgttttggca cgtcgacttc
     3841 gacgcctccg ataagatcaa ctatagaggg aaaagtatca taattcacta gaacgtagta
     3901 gttgatcggc atgccaaggt agtttacaac tgtttcacgt agaagttcga cccctccata
     3961 tgcatacgca tgggcaattt tttcccagcc tcttttcggc agctggacac gcgtatcgcg
     4021 cggtattgac atgagacgaa caatcttatt atcgatatcg atggttgcat atgcgatcgc
     4081 atccgcgcgt cgccctccgt cgacatcgtc aataccgatg aggaggagat ttatagatcc
     4141 cgactcctcc gtaaaagaga tggactccct aattgtctgc ggttgaggct ctacgaacgt
     4201 cttcaccttg aaggctgccc ctgcgaaagc cgctcccatt gccaagagaa gaattgccac
     4261 tatgcgcagt attttcattg tttatcgccc tccagttttt ccggtagagt cctttcttat
     4321 atatatactg ctccaccgtt tcaggaagaa ggtattttat gctcctcccc gtctctatcc
     4381 gctggcggat ttccgtactc gaaatagaga ggagcggaat ttcgagtgga atgattgcat
     4441 cccggagagt cggcggaagc atatccaacg cgttctgggc atatccgggc cgattaaccg
     4501 ccacaatacg gcataacttc gggagttcag cgtattcttt ccaagtcgcc atgctcaaga
     4561 cagcatccag ccccgtaatg aagaaaaact ctacagtttc gggagcatac cagtgacgca
     4621 tctcccgaag agtatcgaca gtatgactcg gttcctttct gtcgatctcg atccgcgaag
     4681 gcgtaaaatt aggattctcc aacgtcgtta agagggtcat gatataccgg tcttcacaga
     4741 aagcaacgcg acgctgacgt ttatgcgccg ggtctccagc aggtacgaaa atcacctcat
     4801 caagaccgac agcagtccgg ctttcttccg cagctaatag gtggccgaag tgaattggat
     4861 cgaaagtccc gcccataatg cctattcttc gcacatcatt gcgtacagaa tacgacacgt
     4921 cgtcaactcc ttcgcttccg actcccctgc ttcatctgtt ctccgaacga gaagcccctg
     4981 ccatatccgg ttcaaagtcg aattcgtgat cgcctataaa taccttatca ccttttactg
     5041 cgcccatttc ctcaagacgt tcctcaatgc ggaacttttt cacgagccgg gcaaacttag
     5101 gaagcgcgtc ctcctgttca aagtcatacc gaagaagcgt tttctcgaga ttcgcatgaa
     5161 caacacggaa tcccgctccg tcgccgagct ttatgattgt gacaggttcg agcttgcgtc
     5221 tccaacgacg aggctcctga atttcaacta caagcggcgc aagcctggtc tcgccttcat
     5281 gccgctggtt ttctctcgcg tactccgcta tgagctgtat gaattccggt ataccttctc
     5341 cgctaagagc gcttactgaa aaatagggta tcccctgctc ctccataaat gaacgaatat
     5401 gcatatcgtt ttccgcagcg ttttcaagat ctattttatt ccctactaca atatacggtc
     5461 tcagtaaaag atcctgtcta tggtatccgg gaagaccgct ctcttcagaa ttgctgtagg
     5521 cctcaaactc gttgcggatg atgctccact gttcgatcac ttcatcagca gtcccggcgc
     5581 tcaagtcgag gacatgaaca agaaaacgcg ttcgctcaac gtgacgaagg aaatagacgc
     5641 caagcccctt gttctcatgg gccccctcaa tgagtcccgg aacatcggca atcacaatct
     5701 tttgatcgtc gactgagaga acccccagat tcggtgaaag ggtcgtgaaa gggtatcccg
     5761 caattttagg gcgtgcagaa gaaatcgctc ctagaaggct ggatttcccg gcattgggta
     5821 cgccgacaag accaacgtcc gcaatcagct tgagctcgag cagcagcgtc cgcgcctcgc
     5881 cttcatcgcc cttttccgcg aaacgtggcg attttcgcac tgagtttgca aagtgcgcgt
     5941 ttccttttcc ggggcgcccg cccttcgccg ctataaggcg atctccaggc tcgacaagat
     6001 cggcaagtat ctcgccggta tcgtcgtcat agacaatggt accgcaggga acgatgacaa
     6061 ttttgtcgtt ccccgctccg cctgatttca taccgccctt gcccggttct ccgtttccgg
     6121 cctggaattt cttttcatat tcaaaatcgg caagcgtcaa cgctcccgga gccgcctcca
     6181 gaatgatgtc gccccctctt cctccgtcgg cgccgtcagg accgcctttc gggataaact
     6241 tttccctacg gaagctaaga cacccgttcc ccccgcgacc tccgcggaca gatattcgtg
     6301 taaaatcaac aaatttcatg tgatcaccgg ccgaaaggcc ttttccttta tcatttcaaa
     6361 aaatcaaggg ccttgatgat cccctaaggg cgagagccga cgtaaaaagc agacggtctg
     6421 ttcttaaggt gatgcgaagg cccttgttga atgcgtcgtg ttcaaaaagt ccggagaatt
     6481 actctgccgc ttgcagaact actgaaacta ttttcctgct cccttttgtc tcgtaacgca
     6541 cggttccagc gcttgtcgca aaaatcgtat agtccttacc aagaccgacg ttcttgccag
     6601 gatggatttt tgttcctctt tggcgaacca aaatgttgcc cgcctgtacc gtctggccgg
     6661 catatatctt tatcccgagc ctctttcctt cgctgtctct tccattcgta ctgcttccct
     6721 gccctttctt gtgggcaaag agctgcaggt caaaattcca tttcatcaac aggcacctcc
     6781 agaatacgga catgctccgg gtatacccgg gctatttcct tcaaggatcc cactatagtt
     6841 tccacgagaa ccgccgtctg ttccggaggg gatcgtctcc agtccaatac cattctcgcg
     6901 ctgtcctcat tgatgctgaa agaaaaaccg ggagaagaaa caaccttctc aaaaccgaaa
     6961 agaagcgcct gtaaaagtgc ggagacgccc gcacatacaa tatcttcgcc ccgttcggca
     7021 taccccgaat gaccttctgc gacaagagcg acagggcgct cctcgtcgaa ggaaacggag
     7081 atctcggtca aaatcaaact ccagcagtca gagtatcgat tcgtatctca gagtaattct
     7141 gtctgtgccc tctcatacgc cgatagtttt tcttgctctt gaacttaaaa acgaggatct
     7201 tcggttctct gccattcccc gtcaccgaag cggtcaccgt agctcccttg acataggggg
     7261 cgccgactac aacagcgtca tcccttccta gaagaaggac cttatcaaaa accaccgagg
     7321 cgccttcttc aagaccgagt gattcgactt tcaaaagatc gccctcctga acacggtact
     7381 gtttgcctcc tgtttcaacg atcgcgtaca ttgttttcct cctcccgccg ggaagaccag
     7441 gcgctgctca gcagcttcaa cctgtcccgt gcgatttaaa catccaaccg tagtattata
     7501 cggagcgaaa aggttaaagt caaacttttc ctcgcagggc agcgagatca ccctctccgg
     7561 caaggacgct tatattgcct gattggctgc actccgtact ctccgcggag tagagaagcc
     7621 gttcggcatg ttctcgagcc tcctgcaaag aggcgtctcc tgagagcatc cgtgctactt
     7681 ctccgatgcg gtcgtctctt tccagctttt tgactt
//
LOCUS       DAOTIL010000021         7556 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminobacteriaceae bacterium isolate AMR_MDS_3026
            NODE_11677_length_7556_cov_3.921477, whole genome shotgun sequence.
ACCESSION   DAOTIL010000021 DAOTIL010000000
VERSION     DAOTIL010000021.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868050
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminobacteriaceae bacterium (wastewater metagenome)
  ORGANISM  Aminobacteriaceae bacterium
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae.
REFERENCE   1  (bases 1 to 7556)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7556)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:19:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,224
            CDSs (total)                      :: 3,198
            Genes (coding)                    :: 3,184
            CDSs (with protein)               :: 3,184
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7556
                     /organism="Aminobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11677_length_7556_cov_3.921477"
                     /isolate="AMR_MDS_3026"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:3034609"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..3171)
                     /locus_tag="P5201_00855"
     CDS             complement(<1..3171)
                     /locus_tag="P5201_00855"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRV97111.1"
                     /translation="MRGKFKKIAGVLFVLCIAAFLAAFFLISGQDAGTSYFKTELEKA
                     VATLAGWTLETDGISGNFLAGYNAANIRILFEGQEVARAEELSVGLSILSFFRGQSGL
                     TKVTVTEGVLSGEGMLHALRRSDFSAGSGITTDFMVPVILFSPVTITTPLGEISLASL
                     RLTPGEKTMTFHGEGHFLDFPVEAGASFTTGEQLSITDGFLRGGNAVVSFSGPLFPET
                     RIEGNIDDLRLDVISELTNLPFTAKGTVDSTLLLERPEGRLLVSGEGEIENGDIWDLL
                     MDGTFSWSADESKAVVAPVDARVFSSPASGVFALYFGEEPTAEIAFALKQLKIEEWTH
                     CFSWLSFAQGTLSTLKIDLSGPFEKLNGPILFEAPKATLEGFDVRALRGDLRMFDGER
                     IAISGNATWSDSPFTISGKSLLDDSAGAETSLSLHSGSFDLKSSGKVYAPELSADGKG
                     TMALEISIPSEGAASYKGALAAPRASFLGTAGEKLSVVFSGTEEILSLSALSFSPAGK
                     GLLSGKGSITGLSGKSPSITMEGSGRDLAWNLLRDALGGLETAGLFDASWSVKGPTAS
                     PVVSFGLKGRETPFSRSLPLRNIQLEGRFEKNELSVSKGSGSLFGGTANIEGKARFGS
                     APSLSFKGAFRGLSAGQIAAAAGADAETADGVVAGEFSLSGKPSSPLLSLTASAPSLG
                     VASLPLTAVRAKAEGTLSSLKITDFSARLQESSVDLSGVIGLSKGEKTNLKIAANKLD
                     LHSLAAAFFPGVRLGGTLSAQLNVSGKTGGAYTSTLSGTSPFLSVYGVLLEKAKVSLT
                     PDGEDAFDVALEGMLGESALALEGRAVVSPSGATFSLKNTKKIDLAKTASALSAHAAG
                     IFTGDADFSLTGKLGAVPTFEGSISAQSLGLYKTEARSVSVPFRWSDGMFVISGGKGV
                     YHEGTALFDGKVDPVTMRWEGNLAVKGMDLASASERILEGQGKISGKADLTMRGSGTG
                     GMLGLVFGSGQISAKEGSVSGFESIKSVSKTGEIRFSSLLASFNMDGRSIFLLPGSRL
                     SAPPGDTVYRYFSASGSVGWNDSPLDM"
     gene            complement(3472..4260)
                     /locus_tag="P5201_00860"
     CDS             complement(3472..4260)
                     /locus_tag="P5201_00860"
                     /inference="COORDINATES: protein motif:HMM:NF012774.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thermonuclease family protein"
                     /protein_id="HRV97112.1"
                     /translation="MRRIALFILLFALFFVLFLFFRAVPFQRDEQPSPPPGVRIFDGD
                     TAELPSGGGVARVRYLLVDTPELHHPSRPAEELGEEARLLNSRLLASGPLRLEYDSER
                     TDRYGRTLAYVFAGGEGSELFLNEILVREGLAQPLIIPPNRRYAGRILKAFEEARAGQ
                     RGLWNRAEGRVFTSAQVWSEAPLLAGSFLTLFMTIEKVELKGRRILLREGRVTVVAYK
                     SPETESLLSLREGDRIRAVGKLLLSYNGCELPVASEIQVSRSTE"
     gene            complement(4257..5015)
                     /locus_tag="P5201_00865"
     CDS             complement(4257..5015)
                     /locus_tag="P5201_00865"
                     /inference="COORDINATES: protein motif:HMM:NF015949.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF364 domain-containing protein"
                     /protein_id="HRV97113.1"
                     /translation="MRLYDSILQEVLKAPDGAVDNLIIGWKGSFLSLSDGRWGVGSTP
                     PEGGEPMSPREEHTRNILMSGAHSLARLLVSPYPQEFSAASASLAALAPAPEGGEPLE
                     FLLQLPGRDRVALLAPDPWVTDFLRDWNWKISIFDDSRRGLGILPEWTTSQHLHSSAV
                     LWINAETLRNRRILSLMPLLKGKRGVILQGPGIPFLPSVFREAGITHLVLPAAEEAAA
                     PETRRYIAAGGTPWTCPSLRWRVFPLLYTDEKNR"
     gene            5115..5789
                     /gene="rsmG"
                     /locus_tag="P5201_00870"
     CDS             5115..5789
                     /gene="rsmG"
                     /locus_tag="P5201_00870"
                     /EC_number="2.1.1.170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013049228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA];
                     GO_process: GO:0046118 - 7-methylguanosine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(527)-N(7))-methyltransferase
                     RsmG"
                     /protein_id="HRV97114.1"
                     /translation="MTESVESLVHVHEKKLREYVSLLSSYEGKIRLTGPSDPEVLWNE
                     HIVDCLHSVPYLPESGRTLDVGSGGGLPGLVWALCRPDLQVTLLDSVRKKCGALAEMA
                     SALQLENVQVVCARCEEYAVKEREVFQCAAARAVAAADVLLEYLAPFVTVSGRVLAFK
                     GPLYVEEIKPMEGKWARLGLSAPEVHTYTAGEKERYLLLWTKQTPVPQRFPRKIGMAE
                     KTKWWR"
     gene            5797..6567
                     /locus_tag="P5201_00875"
     CDS             5797..6567
                     /locus_tag="P5201_00875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013049227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HRV97115.1"
                     /translation="MITLAISNQKGGVGKTTTCINLAAELGRKGYSVLSVDIDPQANC
                     TSGLGVALPPKAPSLYNVLLGDVPPQEAILQTKWKGVSMLPATLDLAGAEIELASSIS
                     RETKMRRVLSRIEGFDIAILDCPPSLGMLTVNALVAADKLVIPIQCEYFALEGLGQLS
                     RTISLVKDGLNQNLGIDGILLTMHDSRTRLSIDVADEVRRQFGDVVFKTFIPRNVKLA
                     EAPSYAEPICYYDPSCSGAKAYEEFAEEVSTLWLKEED"
     gene            6546..7436
                     /locus_tag="P5201_00880"
     CDS             6546..7436
                     /locus_tag="P5201_00880"
                     /inference="COORDINATES: protein motif:HMM:TIGR00180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ParB/RepB/Spo0J family partition protein"
                     /protein_id="HRV97116.1"
                     /translation="MAKGRGLGKGLASLLPAAQTLGEGEPEITMLSMDRILANPFQPR
                     KEINENTLVELADSIRAHGVIQPLIVRRNEDGDYWIVAGERRWRAAKIVGLSEVPVRV
                     IDGNEMEMREITLIENIQREDLSPVDTALALEELLNVYDITQDELAASIGWSRTSVTN
                     KLRLLQLPEEVKTLLSNNELSEGHCRALLPLESQDEIAKLASDAVNAGLSVRQVEDIV
                     KRKNAHSPKEKRPKDVFALPESISQKARSLGFSIRITGKKSKMKLHIEGVHEELVRDV
                     LTYLEENAEKYFPGNTPDEQ"
BASE COUNT         1784 a         2100 c         2206 g         1466 t
ORIGIN      
        1 catgtcgagg ggggagtcgt tccaccctac ggagccgctc gctgagaagt agcgatagac
       61 ggtgtctccc ggaggcgcgg agagtctgct tccggggaga agaaagatgc tgcgtccgtc
      121 catgttgaag cttgcgagga gcgacgagaa tcggatttcc cctgtcttcg acaccgattt
      181 gatcgactcg aaccccgaga cggagccttc cttcgccgaa atctgcccgc tgccgaagac
      241 aagtccgagc ataccgcccg tgccgctccc ccgcatggtg agatcggcct ttccggagat
      301 cttcccctgc ccttcgagga tgcgttccga agcgctcgca aggtccatcc ccttcacggc
      361 gaggtttccc tcccaccgca tggtgacagg gtcgactttc ccgtcgaaga gggcggttcc
      421 ctcgtggtag acccccttgc cccccgaaat cacgaacatg ccgtcgctcc agcggaacgg
      481 gacggagaca ctgcgcgcct ccgttttata aagtcccagc gattgggcgg aaatggagcc
      541 ttcaaacgtc gggacggcgc ccagcttgcc tgtgagcgaa aagtccgcgt ctccggtgaa
      601 gatgcctgca gcgtgcgccg aaagcgcgga tgccgtcttc gcaaggtcga tctttttcgt
      661 gttcttgagc gagaatgttg cacccgaggg cgaaacgacc gctcgtcctt caagcgcgag
      721 ggcgctctcc ccgagcattc cttcaagagc gacgtcgaac gcgtcctctc cgtccggcgt
      781 gagcgagact tttgccttct caagcaacac tccgtagacg gagagaaagg gcgacgtgcc
      841 ggagagagtc gacgtgtacg cgcctcccgt ttttccggag acgttgagtt gcgccgagag
      901 cgttccgccg agacgtaccc cgggaaagaa cgccgcggcg agcgaatgga gatcgagctt
      961 gttcgccgct atcttcagat ttgtcttttc tcctttgctc aggccgataa cacccgaaag
     1021 atcaacagaa gactcctgca gtctggccga gaaatccgtg atcttcaaag atgagagcgt
     1081 tccctcggcc tttgcccgga cagccgtcag gggcaaggag gcaacgccga gcgagggagc
     1141 ggacgccgtc agcgacagca agggggagga tggcttcccc gaaagggaga actctccggc
     1201 gaccaccccg tccgcggtct ccgcatcagc gcctgccgcg gctgctatct gtccggcgga
     1261 gagccctcta aaggctcctt tgaaggagag cgacggcgcc gagccgaaac gcgctttccc
     1321 ctcaatattc gccgttccgc cgaagagaga gcccgagcct ttggaaacgg aaagctcatt
     1381 cttctcgaag cgcccttcga gctggatgtt tctgagaggg agcgagcggg agaacggcgt
     1441 ttctctgcct ttgaggccga acgagacgac cggagaggct gtcggcccct tcacgctcca
     1501 tgaggcgtcg aagaggcccg cggtctcaag accgcccagg gcgtcccgca gaaggttcca
     1561 cgcgaggtcg cgtccgcttc cctccatggt gatggacggc gactttccgg agagtccggt
     1621 aatgctcccc ttgccggaga gaagcccctt tccggcggga gagaaagaga gcgcggagag
     1681 agaaagaatc tcttcagtac ctgaaaaaac tacggaaagc ttctctcctg ccgtacccag
     1741 aaacgaagcg cggggcgcgg caagagcgcc tttgtacgat gcggccccct ccgaaggtat
     1801 ggagatttca agagccatcg tacctttccc atcggcggag agctcgggag cgtagacctt
     1861 tccggaggac ttcaggtcga agcttccgga gtgaagagaa agggaggtct ctgcgcctgc
     1921 cgagtcgtca agaagactct tccccgagat ggtgaaagga gaatcagacc acgtcgcatt
     1981 cccggagatg gcaatgcgct ccccgtcgaa catgcgaaga tctccgcgga gcgcgcggac
     2041 gtcaaagcct tcaagagtcg ccttcggcgc ttcgaagaga ataggtccgt tcagcttctc
     2101 gaaaggaccg gaaaggtcga ttttgagcgt cgagagcgtt ccttgggcga aggagagcca
     2161 ggagaagcag tgcgtccact cttcaatctt cagctgtttc agtgcaaacg caatttccgc
     2221 cgtcggttcc tcgccgaagt agagggcgaa gacgccggat gcgggcgagg aaaaaacacg
     2281 tgcgtcgacg ggagcgacaa cggctttcga ctcgtccgca gaccatgaga acgtaccgtc
     2341 catcagcaga tcccagatat cgccgttttc gatctccccc tctccggaga cgagcaaacg
     2401 tccctcgggg cgttcgagca ggagtgtgga gtcgacggtt ccctttgcag taaagggaag
     2461 gttcgtcagt tccgaaatga cgtccagccg aagatcgtcg atgtttccct cgatgcgcgt
     2521 ctccggaaaa agcggcccgg agaaggagac aacggcgttt cctcctctga gaaaaccgtc
     2581 ggtgatggaa agctgttcgc cggtggtaaa cgaggcgccc gcctcgaccg gaaaatcaag
     2641 gaaatgcccc tctccgtgaa aggtcattgt cttctccccc ggcgtcaggc ggagcgaagc
     2701 cagcgagatc tctcccaagg gggtcgtgat ggttacggga gagaacagga tgaccggaac
     2761 catgaagtct gtggttatgc cgcttccggc ggagaagtcc gagcgacgga gcgcgtggag
     2821 catcccctct cctgagagga cgccctccgt cacggtcacc tttgtcagac cgctttggcc
     2881 acgaaaaaac gagagaatgg aaagccctac ggaaagctct tccgcgcggg caacctcctg
     2941 cccttcgaag agaatgcgga tatttgccgc gttgtaccct gcgaggaaat ttcctgaaat
     3001 accatcagtc tccaacgtcc atccggcgag cgtggcgacg gctttctcaa gttccgtctt
     3061 gaaataggac gtcccggcat cctgcccgga gatcaggaag aatgccgcaa ggaaagcggc
     3121 aatacagaga acaaacaata ccccagcaat ttttttgaat ttcccgcgca tacgctctac
     3181 cctttcccgg agcacaccct cggcgtgcaa tgtaaaaacc atactataat atgcaatcac
     3241 acggggtaat tgcaaggcaa tactactgaa aagaggatga agagaaaaaa gcgcgcgctg
     3301 tacaacctat atattgagcc tctaaggggg cgggggtatt gtggatatgt ggaaaaaact
     3361 gtggataacg ggtgaaaaac agggcgaaag aacaggcctc gcgcgatttt gtggataaca
     3421 tggacttctg tggatagaaa tcacggcaca ctacggctcg ggcgcagcgc gttactcggt
     3481 actccgtgat acctgaattt ctgaagcgac agggagttcg cacccgttgt acgagagaag
     3541 gagctttccg accgcgcgga tacggtctcc ctcgcgaagc gagagcagac tctcggtctc
     3601 gggacttttg taggcgacga ctgtgacgcg tccctcccgc aggaggatgc gccgcccttt
     3661 gagctcaacc ttttcaatcg tcatgaagag agtgagaaac gaaccggcaa gcaacggcgc
     3721 ttcgctccac acctgcgccg aggtaaacac cctcccttcc gccctgttcc aaaggcctct
     3781 ttggccggca cgcgcctcct cgaacgcctt gaggattcgc cccgcgtacc tccggttcgg
     3841 aggaatgatg agaggttgtg caagtccctc ccggacgagt atctcgttaa gaaacagttc
     3901 agagccctca ccaccggcga agacgtaggc aagggtgcgt ccgtacctgt ccgtccgttc
     3961 gctgtcgtac tccaaacgaa gaggtccgct tgcaagcagt ctgctgttca gcaggcgcgc
     4021 ttcctctcca agctcttctg ccgggcggga cgggtgatgc agttccggcg tatccaccag
     4081 aaggtatcgt actcgtgcga cgccccctcc cgaaggaagc tccgcggtat ctccgtcgaa
     4141 gatcctgacc cccggaggcg gagaaggctg ctcgtctcgc tgaaaaggaa cggcgcgaaa
     4201 aaagagaaag agaacaaaga acagcgcaaa aaggagaatg aacaaggcga ttcgtctcat
     4261 ctgtttttct catccgtata taatagaggg aagactctcc agcgaaggga agggcaggtc
     4321 cacggagtac cgcccgctgc gatgtatcgc cgcgtttccg gagcagccgc ttcctccgcg
     4381 gcgggaagga caaggtgcgt aatgcccgcc tctctgaaaa ccgagggaag aaacggtatt
     4441 cctggcccct ggaggatcac tcctctcttc cctttcagaa ggggcataag cgagaggatt
     4501 ctccggttgc gcagcgtttc tgcgtttatc cagagtacgg cgctcgaatg caggtgctgc
     4561 gatgtcgtcc actcgggaag aataccaaga ccgcgccggg agtcgtcgaa gatggaaatc
     4621 ttccagttcc agtcgcgcaa aaagtctgtc acccaggggt caggggcaag aagggcgacc
     4681 cggtccctcc cggggagctg cagaaggaat tccaaaggtt cgcccccctc aggcgccggg
     4741 gcgagcgcgg cgagcgaggc cgacgcagcg gaaaactcct gcggatacgg ggagacgagg
     4801 agccgggcga gactatgggc gccggacatg agaatgttgc gcgtatgctc ttcccggggg
     4861 gacatcggct cgcctccctc gggcggagtg gaaccgacgc cccatcggcc gtccgaaagc
     4921 gagagaaaac tccccttcca gccgatgatc aaattgtcga cggctccgtc gggagctttc
     4981 aggacttctt gtagaatgga atcatacagg cgcatatcgc tcctcctcgc cgaccattgt
     5041 aacgcatacg aggagagaag cagaagcgct actctttgaa aactcaaatt ctgaacgtcg
     5101 aaaggagaag cgtattgaca gaaagtgttg aatcgctggt gcatgtacat gagaagaagc
     5161 tgagggagta tgtatcgctc ctgtcctcgt acgaaggcaa aattcgattg acgggaccga
     5221 gcgaccccga agtcctctgg aatgaacata tcgttgattg cctccactcc gttccgtatc
     5281 taccggaatc gggaagaacg ctcgacgtgg ggtcgggagg aggtcttccc ggtcttgtgt
     5341 gggctctctg ccgccccgac ctccaggtga cgctgcttga cagcgttcgc aaaaagtgcg
     5401 gcgcgcttgc cgagatggcc tccgcgttac agctggagaa tgttcaggtt gtctgcgctc
     5461 gctgtgaaga atacgccgtg aaggaacggg aggtttttca atgtgcagca gcccgtgccg
     5521 ttgccgcagc ggatgtgctc ctggagtatc ttgctccctt tgtgaccgtt tcaggacgtg
     5581 tgcttgcatt caaaggaccg ctctacgtag aggagatcaa gccgatggaa ggaaaatggg
     5641 cgcgcctcgg actctccgca ccggaagtgc acacctatac agccggtgaa aaagagcgct
     5701 accttcttct ctggacaaaa caaacgcctg ttcctcaacg atttccacgg aagataggca
     5761 tggcggaaaa aacaaaatgg tggaggtgat gcgggagtga ttactcttgc aatatcgaat
     5821 cagaaaggcg gagtgggaaa gaccacaaca tgcatcaatc tcgctgcgga gcttggacgc
     5881 aaagggtatt ccgtcctctc ggtcgatatc gacccgcagg caaactgtac gagcgggctc
     5941 ggcgttgcct tgcctccgaa agcacccagc ttgtataacg tcctccttgg cgacgtaccg
     6001 ccgcaggagg caatccttca aaccaagtgg aagggtgttt caatgcttcc ggcgacgctc
     6061 gacctggcgg gggcggagat agagctagcg agttctatca gcagagagac gaagatgcgt
     6121 cgtgtgctct cccgaattga gggcttcgat atcgcaattc tcgactgtcc gccttcactc
     6181 ggcatgctga cggtcaacgc gctggtcgcc gccgataagc tggtcattcc tatacagtgc
     6241 gagtattttg cactcgaagg attagggcaa ctctccagga cgatatcgct cgtaaaagac
     6301 ggactgaatc aaaacctggg catcgacgga attctcttga ccatgcacga ctcaaggacg
     6361 cgtctttcta tcgatgtggc tgatgaagtc cggaggcagt tcggcgacgt cgtcttcaaa
     6421 acattcatcc cgaggaatgt caaactggcg gaagctccaa gctacgctga gccaatctgc
     6481 tattacgacc cctcatgctc aggtgcaaaa gcctatgaag agttcgcgga ggaggtgtcg
     6541 acgctgtggc taaaggaaga ggactaggaa agggtcttgc gtcgctgctc ccggcggcgc
     6601 aaacgctcgg ggagggagaa ccggaaatca caatgctttc gatggatcga attctcgcca
     6661 acccgttcca gccaagaaaa gagatcaacg aaaacactct ggttgaactt gccgactcca
     6721 tcagggcgca cggagtaata caaccgttga tcgtccggag aaatgaagac ggcgactact
     6781 ggatagtcgc cggagagaga cgatggagag ccgcgaagat cgtaggctta agcgaagttc
     6841 ctgtgcgcgt gatcgatgga aacgagatgg agatgcgtga aatcacactc atcgaaaaca
     6901 tacaacgcga agatctctca ccagtggaca ctgcgctcgc actcgaagaa cttctgaacg
     6961 tctacgacat cacacaggat gaacttgccg caagcattgg atggagcaga acgtccgtca
     7021 caaataagct ccgccttctt cagttacctg aagaggttaa aacgctcctc tcgaacaacg
     7081 agctctccga gggtcactgt agagcgcttc tcccgctgga gtctcaggat gagattgcga
     7141 aacttgcctc agacgcagtg aacgccgggt tatctgtgcg tcaggttgag gacatcgtaa
     7201 aacgaaagaa cgcccactct ccgaaagaaa aaagaccaaa ggatgtcttt gcgcttcctg
     7261 aaagcatctc ccaaaaagcg cgatctcttg gattttcaat acgaatcacg gggaagaaat
     7321 caaagatgaa gctgcatatc gaaggagttc acgaagagct tgtccgggat gttctcacat
     7381 atcttgaaga gaacgcggag aagtatttcc cgggaaatac tccagacgaa caatgaaaaa
     7441 ctacgcagat ctgatccttt tcggaaaggt agtatccaca gcactcctcg tggtaggata
     7501 tattttgctc ggttactatt tgggaagaag attggtagat aacggatacc ctacct
//
LOCUS       DAOTIL010000022         7358 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminobacteriaceae bacterium isolate AMR_MDS_3026
            NODE_12064_length_7358_cov_3.334520, whole genome shotgun sequence.
ACCESSION   DAOTIL010000022 DAOTIL010000000
VERSION     DAOTIL010000022.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868050
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminobacteriaceae bacterium (wastewater metagenome)
  ORGANISM  Aminobacteriaceae bacterium
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae.
REFERENCE   1  (bases 1 to 7358)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7358)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:19:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,224
            CDSs (total)                      :: 3,198
            Genes (coding)                    :: 3,184
            CDSs (with protein)               :: 3,184
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7358
                     /organism="Aminobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12064_length_7358_cov_3.334520"
                     /isolate="AMR_MDS_3026"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:3034609"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(17..1288)
                     /gene="serS"
                     /locus_tag="P5201_00885"
     CDS             complement(17..1288)
                     /gene="serS"
                     /locus_tag="P5201_00885"
                     /EC_number="6.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013048384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004828 - serine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006434 - seryl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine--tRNA ligase"
                     /protein_id="HRV97117.1"
                     /translation="MLDIRWIRSNADEVKAYLANRNNDFDIEPLLALDEEKRKLLSET
                     EELKAKRNEGSRKVGMAKSKGEDAVELMEEMRVIGDRIRDIDSTLAEIEEKMNNMLLS
                     IPNRPHESVPVGKDENDNPVVRRWGEPKSFAFEPKAHWDIGEAAGIMDFEKGAALAQS
                     RFTVLKGVGARLERALLNFMLDLHTEKHGYLEVQPPFMVNSKTMQGTGQLPKFADDLY
                     KCENDDLWLIPTAEVPLTNLFAGEILEEEQLPFYCTAYTPCFRREAGAYGRDVRGMLR
                     QHQFDKVEMVKISTPERSYEELEMLTDNAEEVLRLLEIPHRTVCLCTGDMGFGSSKTY
                     DIEVWLPSQDKYREISSCSNCEDFQARRMNTRYRPKGGGKPQFVHTLNGSGIAVGRTL
                     IAVLENYQREDGSVEIPKALVSYMGGVEEIR"
     gene            complement(1281..1772)
                     /gene="ribE"
                     /locus_tag="P5201_00890"
     CDS             complement(1281..1772)
                     /gene="ribE"
                     /locus_tag="P5201_00890"
                     /EC_number="2.5.1.78"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014163054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009349 - riboflavin synthase complex
                     [Evidence IEA];
                     GO_function: GO:0000906 - 6,7-dimethyl-8-ribityllumazine
                     synthase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6,7-dimethyl-8-ribityllumazine synthase"
                     /protein_id="HRV97118.1"
                     /translation="MRVIEGKLTGAGLRFCIVVSRFNSFFSEKLLEGAKDALLRHDVS
                     HHGIDIVWVPGAWEIPLIAKEAALSGKYDAIIALGAVIRGDTTHYEHVCAEMSKGIAS
                     VSMTQRVPVAFGVLTCESMDQAVARSGSKAGNKGFEAAAAAIEMANILKQMRTEKETD
                     QNA"
     gene            complement(1801..3060)
                     /locus_tag="P5201_00895"
     CDS             complement(1801..3060)
                     /locus_tag="P5201_00895"
                     /EC_number="3.5.4.25"
                     /EC_number="4.1.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005662159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003935 - GTP cyclohydrolase II activity
                     [Evidence IEA];
                     GO_function: GO:0008686 -
                     3,4-dihydroxy-2-butanone-4-phosphate synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     3,4-dihydroxy-2-butanone-4-phosphate synthase/GTP
                     cyclohydrolase II"
                     /protein_id="HRV97119.1"
                     /translation="MSALSETETQEKTMFSSIEDAVEDIRNGGMIIVVDDDSRENEGD
                     LVIAACKATTEAINFMVRRARGLVCVPLAKEFARRLRLDPMVREGTDKHGTAFLVSVD
                     AKEGTTTGISAEERAATARLLADTSSRPDDFYRPGHMFPLEAKQGGVLKRAGHTEASV
                     DLVRLAGLPPAGVICEIMNEDGTMARLPQLLAFAAEYGLKIISIEDLIAYRSKRERLI
                     EKIATVELPTVHGSFVAHAYKNVLDENAERVHIALVKGEVNGRMGVLVRVHSECLTGD
                     VFGSLRCDCGPQLSSAMEMVEKEGCGVVLYMRQEGRGIGIAEKLKAYQLQEKGLDTVE
                     ANAALGFAPDLRDYGIGAQILLDLGVQNIRLLTNNPRKVVGLQGYGLEIAERIPLVIP
                     PNKFNERYLGTKEQKLGHMLHICDLLR"
     gene            complement(3084..3773)
                     /locus_tag="P5201_00900"
     CDS             complement(3084..3773)
                     /locus_tag="P5201_00900"
                     /EC_number="2.5.1.9"
                     /inference="COORDINATES: protein motif:HMM:TIGR00187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="riboflavin synthase"
                     /protein_id="HRV97120.1"
                     /translation="MFTGLIEYVGTFVSLSASGTYSRLTVRAPSFAGALTRGQSVAVS
                     GACLTVASFGEDLFSVDVMRETLAKTKFRSLRPGARLNLERALRVGDRLDGHIVTGHV
                     DGVAAVRGIRKGSEGFLLDIELDSEMSSLLVPKGSIAIDGTSLTIADFKGAICSVSLI
                     PETLKATNLGDLRPGDDVHIEMDILGKYVRSLLGAMIPGEFGRKGLPGAGDNDTPSGL
                     SMDLFRKAGWL"
     gene            complement(3755..4855)
                     /gene="ribD"
                     /locus_tag="P5201_00905"
     CDS             complement(3755..4855)
                     /gene="ribD"
                     /locus_tag="P5201_00905"
                     /EC_number="1.1.1.193"
                     /EC_number="3.5.4.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006300979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008703 -
                     5-amino-6-(5-phosphoribosylamino)uracil reductase activity
                     [Evidence IEA];
                     GO_function: GO:0008835 -
                     diaminohydroxyphosphoribosylaminopyrimidine deaminase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     diaminohydroxyphosphoribosylaminopyrimidine
                     deaminase/5-amino-6-(5-phosphoribosylamino)uracil
                     reductase RibD"
                     /protein_id="HRV97121.1"
                     /translation="MTLSRKKIDEYFMRRALSLALRGMERTSPNPLVGCVVERDGVVL
                     AEGWHACCGEAHAERAALEGLDSAAGATIYVNLEPCSHFGRTPPCAPLIVEKGIRRAV
                     VGMIDPDERVSGRGLALLRDAGLDVELGVLEPECRRLNRGFLRKVRLGRPWVTLKGAV
                     GLDGSMALPSGESKWITSGGARAFAHLLRAEHDAVLVGAGTVRADDPELTVRNAWGRS
                     PLRVLLDSTLSVGPGAKILGPSCIVFSSESVSPERVAAVEASGASVVRVPRKEAGLSL
                     ETVLSELVRRGICRLLVEGGPAVISSFLREGVADALSLFLAPRLMGQGKTFTGGLSFS
                     CMGETLALRECGVKRAGDDFLLEGVLACSPDL"
     gene            complement(4830..6320)
                     /locus_tag="P5201_00910"
     CDS             complement(4830..6320)
                     /locus_tag="P5201_00910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009201154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0009381 - excinuclease ABC activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrC"
                     /protein_id="HRV97122.1"
                     /translation="MAQEHIKKLLKNLPDKPGVYLMRDAEGEVLYVGKAKSLKKRVAS
                     YFRHESFASPRLRKLVASIEDISVIRTETEVEALILEAKLIKKYAPFFNVELKMGDRY
                     PYIKITSEPFPRIVVTRQRGKDDGLYFGPYVRVGDVRSLLRLIERYFPLRICAREIDP
                     ADAAKGKNRPCLHHALGRCMGVCAGLVTPAEYRERVDDVIFLLQGQSTEVVERLRKRM
                     DKAATSLQFEEAARLRDTIRAIWRITRQRVSSPLREDFDGDMWQCLNRLQELLKLPVL
                     PWRIDGFDISHMSGRETYGVVVVFEQGVPNRSLYRRFKIRTVEGVDDFRSMEETLERR
                     YGKSLEGKEPLPQLILIDGGPVQLEFARKALGKLGLEQIPSISLAKEEELIYHSTERE
                     PLRLDRGDEALRLLQRVRDESHRFAIESHRSSRSARLRRSILEDIPGIGKHRAAQLLG
                     RFGSARAIANMSAEELAAAPGIGGATAARILNFLKEQDSDPLQEED"
     gene            6435..>7358
                     /gene="cysS"
                     /locus_tag="P5201_00915"
     CDS             6435..>7358
                     /gene="cysS"
                     /locus_tag="P5201_00915"
                     /EC_number="6.1.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012869759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004817 - cysteine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006423 - cysteinyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine--tRNA ligase"
                     /protein_id="HRV97123.1"
                     /translation="MSLVLYNDLTRTKEPFVPLKEGHVGFYSCGPTVYDFFHIGNARP
                     FIVFDVLRRYLEYSGYKVTFVQNFTDIEDKMINRANQEGITVKQLADRFIEEYFKDAD
                     ALGIRRATHNPKATEHIPEIIALIEKLVEKGHAYAADGDVFFDVGSFPSYGVLAKQSL
                     EELQSGARVEINERKRHPLDFSLWKAKKEGEPSWPSPWGEGRPGWHIECSAMSMKYLG
                     ETLDIHSGGTDLTFPHHENEVAQAEAATGKPFVRYWIHNGYLLIDKEKMSKSLGNFLT
                     ARAAFQKYPAKAIRLFMLSAHYRSPINFSEES"
BASE COUNT         1501 a         2432 c         1927 g         1498 t
ORIGIN      
        1 ccgttctttg ccgaggttac cggatttcct ccacgccgcc catataggaa acaagcgcct
       61 tcggaatctc cacggaaccg tcctcgcgct gatagttctc gagaacggcg atgagcgtcc
      121 gtcccaccgc gatgcccgag ccgttgagcg tgtggacgaa ctgcggcttg cccccgccct
      181 tcggacggta gcgcgtattc attcttctgg cctggaagtc ctcgcagtta ctgcacgaac
      241 tgatctcccg gtacttatcc tgggagggca gccacacttc gatgtcgtat gtcttgctgg
      301 agccgaaccc catgtcgccc gtgcagagac agacggtccg gtgcggaatt tcgagcaggc
      361 ggagcacctc ttccgcattg tccgtcaaca tctccagctc ctcgtagctt ctctccggcg
      421 tgctgatctt gaccatctcg accttgtcga actggtgctg ccgaagcatt ccgcgcacat
      481 cccgcccgta cgctccggcc tctctgcgga aacacggcgt ataggccgtg cagtagaacg
      541 gaagctgctc ctcctccaga atctcgcctg cgaagaggtt ggtgaggggg acctccgcgg
      601 tcgggatcag ccacagatcg tcgttctcgc acttgtagag gtcgtcggcg aacttcggga
      661 gctgccccgt tccctgcatc gttttgctgt tcaccatgaa gggcggctgc acctcaaggt
      721 agccgtgctt ttccgtgtga aggtccagca tgaagttgag gagcgcgcgt tcaagacggg
      781 ctcccacacc tttcagtacg gtaaatctgc tctgggccag cgccgcgccc ttttcgaagt
      841 ccatgatacc cgccgcctcg ccgatatccc agtgcgcttt cggttcgaaa gcaaaagatt
      901 tgggctctcc ccagcggcga actaccggat tgtcgttctc gtccttccct acggggacgg
      961 attcgtgcgg acgattggga atggagagaa gcatgttgtt catcttctct tcaatctccg
     1021 caagcgtgct atcgatatcg cggatcctgt cgccgatgac gcgcatctcc tccatcagct
     1081 cgactgcgtc ctcacctttg ctcttcgcca taccgacctt gcgcgagccc tcgttccgct
     1141 tcgctttcag ttcctcggtc tcggagagca gtttccgttt ctcttcgtcg agcgcaagaa
     1201 gaggctctat atcgaagtcg ttgtttctgt tggccaggta ggccttcact tcatcggcgt
     1261 tggaacgaat ccatcggata tcaagcattc tgatcagtct ccttttcagt ccgcatctgc
     1321 ttcaatatat tcgccatctc gatagcggcc gccgccgcct cgaacccttt gttaccggcc
     1381 ttgcttcccg aacgggcgac ggcctgatcc atcgactcgc acgtcaaaac gccgaacgcc
     1441 accggcacac gctgcgtcat gctgacggac gctattcctt tcgacatctc cgcgcagacg
     1501 tgttcgtagt gcgtcgtatc gcctcgtatc acggcgccga gcgcgataat tgcgtcgtac
     1561 ttgccgctca gcgcggcctc tttcgcgatc agggggatct cccacgctcc cggaacccat
     1621 acgatgtcga tgccgtgatg agacacatca tggcgcaaca gcgcgtcctt cgctccttcg
     1681 agcagcttct cgctgaagaa gctgttgaaa cgcgaaacaa cgatgcagaa ccgaagcccc
     1741 gcccccgtca gttttccttc gataaccctc attcggaact ctctcccttc aggatctccc
     1801 ttaccgcaga agatcgcata tatgcagcat gtggccgagc ttttgctcct tggttccgag
     1861 gtagcgctcg ttgaacttgt tgggcggaat caccagcggt atccgttccg cgatctcaag
     1921 cccgtacccc tgaaggccga ccaccttcct cggattgttt gtcagcagcc tgatattctg
     1981 taccccaaga tccagaagaa tctgcgcccc gatgccgtaa tcgcgaagat cgggagcaaa
     2041 accgagcgcg gcgttcgcct ccacggtgtc cagccccttc tcctggagct ggtacgcctt
     2101 cagcttctcc gcgatgccga tgccgcgccc ctcctgccgc atgtagagga caacgccgca
     2161 cccttccttc tccaccatct ccatagcgga ggagagctgc ggaccgcagt cgcagcggag
     2221 agaaccgaag acgtcgcccg taaggcactc ggaatgaacg cgcactaaaa cgcccattcg
     2281 tccgtttact tcccccttca cgagtgcgat atgcacccgc tccgcgttct cgtccagcac
     2341 atttttgtac gcgtgcgcca cgaacgaacc gtgaaccgta ggaagctcga ccgtcgcgat
     2401 cttctcaatc aggcgttccc tcttgcttct gtacgctatc aggtcttcga tcgaaatgat
     2461 cttcagcccg tattccgcgg caaatgcaag cagctgcggg agccgggcca ttgtgccgtc
     2521 ctcgttcatt atctcgcata ttaccccggc gggcggaagc ccggcgaggc ggacgagatc
     2581 caccgacgcc tccgtgtggc cggcgcgctt cagcacgccg ccctgtttcg cttcaagagg
     2641 aaacatgtgc ccaggacgat aaaaatcatc cggcctggac gacgtatccg caagcagacg
     2701 cgccgtcgcg gctcgctcct cggccgaaat ccccgttgtc gtcccctctt ttgcgtcgac
     2761 cgaaacgagg aaggccgttc catgcttatc cgtcccttcg cgcaccatgg gatcaagacg
     2821 aagacggcgc gcaaattcct tcgcaagagg aacgcataca agcccccgcg cccttcgcac
     2881 catgaaattg atcgcctcgg tggtcgcctt gcaggcggcg atgacaaggt ctccctcgtt
     2941 ctcacggctg tcgtcgtcga cgacaatgat catcccgccg ttccgtatat cctcgacggc
     3001 gtcctcgatg gagctgaaca tcgttttctc ctgcgtttcc gtctccgaaa gagcgctcat
     3061 tccataacaa cctcccgcac tgtttacagc cacccggcct ttctgaacaa atccatggaa
     3121 agcccggacg gcgtgtcatt atcgccggcc cccggcagcc ccttcctgcc gaattcgccc
     3181 ggaatcatcg ctccgagcag actgcggacg tattttccaa gaatatccat ttcgatgtgc
     3241 acatcatcac cgggccgcag atcgccaagg ttcgtcgcct tcaaggtctc cggaataagg
     3301 cttaccgaac agatcgctcc cttgaaatcc gcgatcgtca aacttgttcc gtcgatcgct
     3361 atcgacccct tcggcacaag gagcgagctc atttccgaat caagctctat atccagcagg
     3421 aaaccttcgc ttcccttgcg tattccccgg acagcggcga cgccgtccac gtgccccgtg
     3481 acaatgtgcc cgtcgaggcg gtcgccgacg cgaagagccc gctccaggtt cagccgggcg
     3541 ccggggcgaa gcgagcggaa ttttgtcttc gcgagcgtct cgcgcatcac atcgaccgaa
     3601 aagaggtctt ctccgaaaga agcaaccgtc aagcacgctc ccgaaaccgc cacggactgg
     3661 ccgcgcgtca acgctccggc aaacgacggc gcccgtacgg taagccttga gtacgttccg
     3721 cttgccgaaa gcgagacaaa cgtccctaca tactctataa gtccggtgaa catgcaagca
     3781 ccccctcaag cagaaaatca tcgcccgcac gcttcacgcc gcactcccgg agtgcaagag
     3841 tctctcccat gcaggagaag gagagtccgc ccgtaaaggt cttcccctgc cccatcaatc
     3901 ggggagcgag gaagagagaa agagcgtcgg ccactccctc tctgaggaag gaggaaatga
     3961 cggcgggccc cccctcgacg agcagtctgc atatacctct gcgcaccagc tcggagagaa
     4021 ccgtttccaa agagagaccg gcctcttttc gcggtactcg cacgacagaa gctcctgaag
     4081 cctcgactgc ggcaacccgt tccggagaaa ccgactccga cgagaagacg atgcacgacg
     4141 ggccaaggat cttcgctccc gggccaacag agagcgtgga atcgagcagc actctcagag
     4201 gcgatcgccc ccacgcgttg cgcacggtca actcggggtc gtccgcacgg acggttccgg
     4261 cgcccaccag aacagcgtcg tgttccgccc tcagaagatg agcaaaagcg cgcgcccccc
     4321 ccgaagtaat ccacttgctc tccccggaag gaagggccat cgaaccgtcg agcccgaccg
     4381 cccccttcag cgttacccac ggtcttccca gtcggacctt ccgaagaaag ccgcgattca
     4441 gccggcggca ctccggctcc aaaaccccta attcgacgtc gagtccagca tcacggagca
     4501 gtgccaagcc tcggccgctg acacgctcat cgggatcgat catcccgacc acagccctcc
     4561 tgataccctt ctcgacaatc agcggggcgc acggaggggt tctgccgaaa tgagaacacg
     4621 gctccaggtt cacgtagatc gtcgcccccg cagccgaatc gaggccttcg agcgccgcgc
     4681 gctccgcatg cgcctcgccg cagcatgcgt gccacccctc ggcaaggacg acgccgtccc
     4741 gttccacgac gcaccccacc aaagggttgg gcgatgtacg ctccataccc ctgagagcga
     4801 ggctcaaagc ccttcgcatg aaatattcgt caatcttctt cctggagagg gtcactgtct
     4861 tgctccttta aaaagttcag aatcctggcc gcggtcgcac cgccgattcc cggcgccgcc
     4921 gccagctctt ccgccgacat gtttgcaata gcacgcgcgc ttccgaaacg cccaagcagt
     4981 tgcgccgccc ggtgcttgcc aatgccggga atatcctcga gaatgctccg ccgcaaccgc
     5041 gccgaacgcg acgaacgatg gctctcgatg gcaaaacggt gcgactcgtc gcggactctc
     5101 tgcaaaaggc ggagcgcttc gtccccccta tcaagccgca gaggttcccg ctccgtgcta
     5161 tggtatatca gttcctcctc ttttgccagc gatatcgagg gaatttgttc caatccgagc
     5221 tttccgagag cctttcgcgc aaattcaagc tgcacggggc ctccgtcgat caggatgagc
     5281 tgcggcagcg gttccttgcc ttcaagcgac ttaccgtaac ggcgttcgag cgtttcctcc
     5341 atcgagcgga agtcgtccac tccctcgacg gtcctgattt tgaaacggcg gtacagcgaa
     5401 cggttaggga ctccctgttc gaacaccacc accacgccgt acgtctctct ccccgacatg
     5461 tgggagatat cgaagccgtc gatcctccac ggaagcacag gaagcttgag cagctcctgc
     5521 aaccggttca gacactgcca catatccccg tcgaagtcct cgcgcagcgg cgaggagact
     5581 ctctggcggg tgatgcgcca aatcgcgcgg atggtatcgc gcagccgcgc agcttcttca
     5641 aattgcagac tcgtcgccgc cttgtccatc ctcttccgga gacgttccac aacctcggtg
     5701 gactgcccct gcagcagaaa aatgacgtcg tcgacccgct ccctgtactc ggccggagtc
     5761 accagcccgg cgcatactcc catgcagcgc cccagcgcat gatgaagaca gggacggttc
     5821 tttcccttcg ccgcatcggc cggatcgatc tcccgggcgc atatccgcag cggaaagtac
     5881 cgttcgatca ggcggagaag cgaacggaca tcgccgaccc gcacgtaggg accgaaatag
     5941 agcccgtcgt ccttacctct ctggcgcgtc accacgattc gagggaaggg ctcggaggtg
     6001 attttgatgt acgggtacct gtcccccatt ttcagctcga cgttgaagaa gggggcgtac
     6061 ttcttgatca gcttcgcctc aaggatcagc gcctcgacct cggtctcggt ccgaatcacc
     6121 gaaatgtcct cgatggaagc caccagcttc ctcaagcggg gcgaggcgaa cgactcatgc
     6181 cggaagtacg acgccacccg ttttttcagc gacttcgcct tccccacgta gagcacctct
     6241 ccctcggcgt cccgcatcag atagactccg ggtttatccg gcagattttt caacagcttc
     6301 tttatgtgct cctgcgccac gtcttcctcc gaatttcaca tgcggctctg ttcgccgagt
     6361 gaagaaaaat gttctgcatc ctaatgaatt gtattatata atacgtgatt agaacgcatt
     6421 ggaggtgctg aaaaatgagt ctggtactgt ataacgatct gacacgcaca aaagaaccgt
     6481 tcgttcctct gaaggagggg catgtgggct tctacagttg cgggcctacg gtctacgact
     6541 tcttccatat aggcaatgcg cgcccgttca tcgtcttcga cgtcctccgc cgctaccttg
     6601 agtacagcgg ctacaaagtc accttcgtcc agaacttcac cgatatcgaa gacaagatga
     6661 tcaaccgggc gaaccaggag ggcatcactg tcaagcagct tgcggaccgt tttatcgagg
     6721 agtatttcaa ggatgcggac gccctgggca ttcgccgcgc gacgcacaat ccgaaagcga
     6781 cggaacacat acccgaaata atcgcgctca tcgaaaagct ggtcgaaaaa ggacatgcat
     6841 acgctgccga cggcgacgtc ttctttgacg tgggcagttt tccctcctac ggagtcctcg
     6901 caaaacagag ccttgaggag ctccagtccg gcgcgcgggt cgagatcaac gaacgcaaac
     6961 ggcatcctct cgacttctcc ctctggaaag cgaaaaaaga aggcgaacct tcctggccca
     7021 gcccgtgggg cgagggacgc cccggctggc acatcgaatg cagcgccatg tccatgaaat
     7081 accttggaga aactctcgat attcactccg ggggaaccga ccttactttc ccgcaccacg
     7141 agaacgaagt ggcccaggcg gaggccgcca cgggcaaacc tttcgtgcgc tactggatcc
     7201 ataacggcta tctcctgatc gacaaggaga agatgtcgaa gtcgctcggc aacttcctca
     7261 ccgcccgggc agcgtttcaa aagtatcccg cgaaggcaat ccgtctcttc atgctgagcg
     7321 ctcactacag gtcgcccatc aacttttcgg aggagagc
//
LOCUS       DAOTIL010000023         7315 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminobacteriaceae bacterium isolate AMR_MDS_3026
            NODE_12169_length_7315_cov_4.004408, whole genome shotgun sequence.
ACCESSION   DAOTIL010000023 DAOTIL010000000
VERSION     DAOTIL010000023.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868050
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminobacteriaceae bacterium (wastewater metagenome)
  ORGANISM  Aminobacteriaceae bacterium
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae.
REFERENCE   1  (bases 1 to 7315)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7315)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:19:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,224
            CDSs (total)                      :: 3,198
            Genes (coding)                    :: 3,184
            CDSs (with protein)               :: 3,184
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7315
                     /organism="Aminobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12169_length_7315_cov_4.004408"
                     /isolate="AMR_MDS_3026"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:3034609"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..564
                     /locus_tag="P5201_00920"
     CDS             <1..564
                     /locus_tag="P5201_00920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006300799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-2 protease family protein"
                     /protein_id="HRV97124.1"
                     /translation="AVLWAITFHEFCHGYMAYRLGDPTAERAGRLTLNPLRHLDPVGA
                     LMLLVFRFGWAKPVPVDPRYFKNPRRDMLLVSIAGVSGNFFTAFVFGMLARFFPYVFL
                     ANAALRQFVVLMILINLGLAVFNLIPIPPLDGSKLLYPLLPSFMLEKYFWLERYGFFV
                     LMILMALGVVQAIMGPIVMFFFRLFLF"
     gene            580..1365
                     /gene="surE"
                     /locus_tag="P5201_00925"
     CDS             580..1365
                     /gene="surE"
                     /locus_tag="P5201_00925"
                     /EC_number="3.1.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013048561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008252 - nucleotidase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5'/3'-nucleotidase SurE"
                     /protein_id="HRV97125.1"
                     /translation="MKLLITNDDGVFAPGIITLASFLAERGEECTVVAPDRERSSVGH
                     AITLTRPLRLWAIPSGLYSSTCSVYACDGTPSDSVMLGLEEIIPEPDLVLSGINKGPN
                     LGDDLTYSGTVSAAMEALILGRPSLAFSLNCRDADEEQHYETAAAVAGSLLDFLRGSP
                     LPEGVLLNVNVPNVPLPLLKGIKVTRKGVRLYEGKVTRLKDPAGRSYFWVSGRPEDRL
                     EEGSDVWAVAGGFVSVTPVHMDMTHFPSLQTLTANGAEKINFK"
     gene            1681..2907
                     /locus_tag="P5201_00930"
     CDS             1681..2907
                     /locus_tag="P5201_00930"
                     /EC_number="6.3.4.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013048560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004055 - argininosuccinate synthase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006526 - arginine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate synthase"
                     /protein_id="HRV97126.1"
                     /translation="MTKKGKVVLAYSGGLDTSVAVVWLKEQGYDVITMTADVGQQAVD
                     LEAAKSKALRTGAEKAYVLDLKKQFVEKFVWPSLKANGMYQSVYPLNSALSRPLIAEV
                     MAEIAKKEGAVAVAHGCTGKGQDQVRIEVCTNALNPDLAVLAPVRDWHFSREAEMEYA
                     QAHGIPVQATHASPYSIDDNIWGRSIECGILEDPWNEPPSDAYTLTCEPHEAPDQPEY
                     VEILFEKGIPVALDGVRMDGPTLIDKLNCQAGKHGVGRIDMLEDRLVGFKSREVYECP
                     AAITLITAHRALETLTLDKRVLATKKELEVKFGELAYEGYWFSPLKDAINSFIDTTQE
                     YVNGVVKVRLYKGMAVVRGMKSGKSIYSETLATYSENDAFDHSAAVGFIKIWGLPIKT
                     WRQVHESKEAIEIPAQ"
     gene            complement(2958..3479)
                     /gene="purE"
                     /locus_tag="P5201_00935"
     CDS             complement(2958..3479)
                     /gene="purE"
                     /locus_tag="P5201_00935"
                     /EC_number="4.1.1.21"
                     /EC_number="5.4.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014163303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009320 - phosphoribosylaminoimidazole
                     carboxylase complex [Evidence IEA];
                     GO_function: GO:0004638 - phosphoribosylaminoimidazole
                     carboxylase activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide mutase"
                     /protein_id="HRV97127.1"
                     /translation="MVSPEVGILIGSKSDYPLVKKVEDVFAFFGVSWEISVASAHRTP
                     DDVVAYVNNAPKRGIKVLVAAAGLSAALPGVVAAHTRLPVIGIPVNAGTVGGLDALLS
                     VAQMPPGVPVASVGIDGVKNAALFAVRILALLREDLAGKLDAWSKKEEDAVRTARNDL
                     EGAHSAPGEAFTL"
     gene            complement(3487..4791)
                     /gene="purD"
                     /locus_tag="P5201_00940"
     CDS             complement(3487..4791)
                     /gene="purD"
                     /locus_tag="P5201_00940"
                     /EC_number="6.3.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008711478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004637 - phosphoribosylamine-glycine
                     ligase activity [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylamine--glycine ligase"
                     /protein_id="HRV97128.1"
                     /translation="MTGKKNILVIGSGAREHALVHTLSRSGTVREIHAAPGNPGMLPL
                     AKLHPVSDAACASILPLVKRHGIDLVVVGPEAPLVAGLADELRSAGVEVFGPGRAGAL
                     LEGSKVHAKKFMDRWGVPTAPWDECTSLQDVEEALAKRSAPFIVKADGLAAGKGVFVT
                     KTREEAFLAAENLLVRGALGEAGKRIIIEDALSGEELTVLAVSDGATYRILPPSQDHK
                     RVYEEDKGPNTGGMGAYSPVPWVGEELLERVRRSILDPTFDGLRHDGVPYCGVLYAGL
                     MVAPDGIPRVIEYNVRFGDPEAQVVLPLLEVDFAEMLFACCEGRLSEFRWEEPSKWAV
                     DVVLASGGYPGTFEKGKEIEGLENVSSMEDFVLFHGGTTFSPEGKILTEGGRVLSAVG
                     LGASLEEALVNAYAGVEQISFENMHYRRDIARRAIIQRRGGI"
     gene            complement(4811..>5600)
                     /locus_tag="P5201_00945"
     CDS             complement(4811..>5600)
                     /locus_tag="P5201_00945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008521757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="bifunctional
                     phosphoribosylaminoimidazolecarboxamide
                     formyltransferase/IMP cyclohydrolase PurH"
                     /protein_id="HRV97129.1"
                     /translation="NNILDADCAMRGCALLQNCCGALVIKHTTPCGMACGETPFEAYE
                     RAYGCDPVSAYGGVVGISRKVDMETVLALADRFTDVLVAPEYDEETVALLAEKKPALR
                     VLKWKGGRVLTDQITGTWSGLLVQADSLPPVPELEKGEWVGAPRPDLWDDLLLAWKAA
                     AISKSNAIALVKNGEAIGIGRGCCSRLHAVDFAVRQAGQKAKGSVLGSDAFFPFTDGV
                     ELAADAGVAAIIQPGGSVRDKDVCAAVERRGISMFITGWRTFRH"
     assembly_gap    5601..5610
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<5611..6306)
                     /locus_tag="P5201_00950"
     CDS             complement(<5611..6306)
                     /locus_tag="P5201_00950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009164880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     phosphoribosylaminoimidazolecarboxamide
                     formyltransferase/IMP cyclohydrolase PurH"
                     /protein_id="HRV97130.1"
                     /translation="MKVRRALLSVYDKTGITELAKTLTDLGWELLSSSGTAEHLRKAG
                     LLVTEVADVTGYPHILGGRVKTLHPKVFGGILARRENGGDLKDVEEHDLPLIDMIVCN
                     LYPFEQAAKKHLPLDDLLENIDIGGVSLLRAAAKNYRQVVALMEPEDYPTVAEELRSE
                     GDVTLPTREQLAIKAFAGTASYDGMIVSGLKETTGYASPALPAKIPLILQKQQTLRYG
                     ENPHQEAALYLPAL"
     gene            complement(6303..6896)
                     /gene="purN"
                     /locus_tag="P5201_00955"
     CDS             complement(6303..6896)
                     /gene="purN"
                     /locus_tag="P5201_00955"
                     /EC_number="2.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005662414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004644 - phosphoribosylglycinamide
                     formyltransferase activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylglycinamide formyltransferase"
                     /protein_id="HRV97131.1"
                     /translation="MTSFCIVLSGRGSNMEALADAVRDGRLDARVEFVASDAPDAPGL
                     EKAHRRGIDTVVLPYASEGKENAERRLSGLCSARGVEWIVLAGFMRILSPEFVTAHAG
                     RIVNIHPSLLPSFPGRDGIGDAWRFGVAVTGVTIHLVDAGIDSGRILAQRAVEIFPED
                     SLENLEEKIHTTEHALYWKTLSELFRTGVPAERRWRR"
     gene            complement(6893..7237)
                     /locus_tag="P5201_00960"
     CDS             complement(6893..7237)
                     /locus_tag="P5201_00960"
                     /inference="COORDINATES: protein motif:HMM:NF014788.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AIR synthase-related protein"
                     /protein_id="HRV97132.1"
                     /translation="MLLAPTKLYVNTALRAVSTGKVKAMAHITGGGLADNLRRVVPDG
                     LGLSLDYTAWPRPAIFNLLSEEGVEEEEMRRVFNLGIGYCLIVAQEDVSLVLEQIEAQ
                     REQGIIIGEVVA"
BASE COUNT         1555 a         2088 c         2021 g         1641 t
ORIGIN      
        1 gctgtccttt gggcaattac atttcatgag ttctgccatg ggtatatggc gtaccgactc
       61 ggcgatccga ctgcggagag ggcgggacgt ttgacgctga atccgctccg tcatcttgac
      121 ccggtaggag cgttaatgtt gcttgtcttc cggttcggat gggcgaagcc ggttccagta
      181 gatccgaggt attttaaaaa tcccaggaga gacatgcttc tggtctccat cgccggggtt
      241 tccggaaact tttttacagc cttcgttttt ggcatgctcg cgcgcttttt cccgtatgtt
      301 tttctggcaa acgctgccct caggcaattt gtggttctga tgattttgat aaacctcggc
      361 ttggcggtgt tcaacctcat cccgattcct cctctcgacg gctcgaagct gctttatccg
      421 ttgctgccct ccttcatgct cgaaaaatac ttctggctgg agcgttacgg cttctttgtc
      481 ctgatgatac ttatggctct cggagttgtt caggcaatta tgggaccgat cgtgatgttt
      541 ttcttccgtc tctttctttt ctaggagaag tcgaatcgca tgaaacttct tataacgaac
      601 gacgacggcg tttttgcacc cggaataatc accctcgcct cttttctggc ggaacggggt
      661 gaagagtgca ctgtggtagc tccggacagg gaacgaagca gcgtcggcca cgctattact
      721 ctcactcgcc ctctccgctt atgggcaatc ccatcggggc tctattcctc tacgtgttca
      781 gtgtatgcgt gtgatgggac cccctccgac agcgtcatgc tgggattgga ggaaatcatt
      841 ccagaaccgg atctcgtgct ttccggcatc aataaaggcc caaacctggg cgatgatctc
      901 acctattccg gcactgtttc cgcggcgatg gaggcgctga ttcttggacg cccttcgctt
      961 gcattctctc tgaattgcag ggacgcagac gaggagcaac attacgaaac agccgccgct
     1021 gtcgccggat cgcttctcga ttttttgcgg ggttctccgc tccccgaagg ggtgctgctc
     1081 aacgtaaacg tccctaatgt accgcttcct ctgttgaagg ggataaaagt gacgcggaag
     1141 ggagtgcgtc tttacgaggg caaggtaacg cgcctgaagg accccgcagg gcgctcctat
     1201 ttctgggtct ccggaagacc ggaggacagg ctggaagagg ggagcgacgt ttgggcggtt
     1261 gccggcggat tcgtctcagt aaccccggtg catatggaca tgacgcactt cccctcttta
     1321 cagacgctga cggcgaatgg agcagaaaaa ataaacttca agtgagtgat ttcaacttac
     1381 ttgacaaatg tttcgcagtc ggtataatat ccctactcaa gcgtattggc gatgatgagg
     1441 aagagtactc cgcagcacag caacagagag gacgggccac ggctgagagc ccttccggca
     1501 ggtcacggag gaagagagcc ttggagccgg tttcgaagaa aggcctcggc caagtagaaa
     1561 cggacagaga gggcggaaat tccgtcaaaa agggtggtac cgcgggttca agactcgtcc
     1621 ctggtaaatt ggggcgagtc tttatttatt tagttcgact attcttttgg agggataaaa
     1681 atgacaaaaa aagggaaagt tgtccttgca tacagcggcg gtttggatac ttcagtggcg
     1741 gtggtatggc tcaaggagca ggggtatgac gttatcacga tgacggccga tgtagggcag
     1801 caagccgttg atcttgaggc cgcgaagagc aaggctcttc gtactggcgc cgaaaaagcc
     1861 tatgttcttg acctgaagaa acagtttgtg gagaaattcg tatggccttc attgaaggcc
     1921 aacgggatgt atcagagcgt ctatccgttg aactccgcgc tctcgcgccc gcttattgcc
     1981 gaggttatgg cggagatcgc aaaaaaagaa ggcgccgtcg cggtcgccca cggctgcaca
     2041 ggcaaaggac aggatcaggt gcgcatcgaa gtgtgcacca acgcgcttaa tcctgatctc
     2101 gcggtgcttg cccctgtcag ggactggcac ttctcccgcg aagccgagat ggagtatgcg
     2161 caggctcacg gcatccccgt tcaggcgacc catgcctccc cgtacagcat cgacgacaat
     2221 atctgggggc gctccatcga atgcggaatt ttggaggatc cgtggaacga acctccttcc
     2281 gacgcctaca cactgacctg cgagcctcat gaagccccgg atcagcctga atatgtggag
     2341 attctgttcg agaagggaat tccggtcgct ctggacggcg ttcgtatgga cggcccgacg
     2401 cttattgata aactgaactg tcaggcggga aaacatggag tagggcggat cgacatgctc
     2461 gaagacagac ttgtcggttt caagagccgc gaggtttatg agtgtccggc ggctatcaca
     2521 ctgattaccg cgcatcgcgc ccttgagact ctcactcttg acaaacgcgt tcttgcaacg
     2581 aagaaagaac ttgaagtcaa attcggcgaa cttgcctacg aagggtattg gttctccccg
     2641 ttaaaggacg cgatcaactc cttcatcgac accacgcagg agtatgtcaa cggcgtggtc
     2701 aaagtccgtc tgtataaggg aatggccgtg gttcgcggaa tgaaatccgg taagtccatt
     2761 tacagcgaga ctcttgccac ttactcggaa aacgatgcat tcgaccactc tgcggcagtc
     2821 ggcttcatca agatctgggg cctgccgatt aaaacgtgga ggcaagtcca cgagagcaag
     2881 gaagccatcg aaatccctgc acagtaaaag gacaagaaaa aacggaggaa ccgaggatat
     2941 cccggttcct ccgtttttta cagagtaaac gcctcgccgg gagcgctatg cgccccctca
     3001 agatcgtttc gcgccgttcg gacggcgtct tcctcttttt ttgaccacgc atcaagcttg
     3061 cccgcaagat cctctcgtag aagcgcgagg atacgtaccg cgaaaagcgc cgcattcttc
     3121 accccgtcta tgcccactga agccaccggc acgccggggg gcatctgcgc taccgagaga
     3181 agcgcgtcaa gtcctccgac agttccggcg ttcaccggaa tgccgatgac aggaaggcgc
     3241 gtgtgcgccg caacaacgcc cggaagggcg gctgagagcc ccgcggcggc cacgaggact
     3301 ttgattccgc gtttcggcgc gttgttcacg taggcgacga cgtcatccgg tgttctgtga
     3361 gcggatgcaa cggaaatctc ccaagaaact ccgaaaaaag caaagacatc ctcgactttc
     3421 ttgaccaagg gataatccga ttttgatccg atcagtatcc caacttctgg tgaaaccaac
     3481 ggcatatcag atgcctcctc ttctctggat aatcgccctt cgggcaatgt ctctcctgta
     3541 gtgcatattt tcaaaggaaa tttgttcaac acccgcatag gcgtttacga gagcttcctc
     3601 cagcgatgcc ccgaggccga cggcgctgag gacgcgtccg ccctctgtga gaatctttcc
     3661 ctcgggagag aaggttgtgc ctccgtggaa aagaacaaaa tcttccattg atgaaacatt
     3721 ctctagtccc tctatctctt tccctttctc aaacgtcccg gggtatcccc cggaggcaag
     3781 caccacatcc accgcccatt tggacggctc ttcccaccgg aactctgaaa gacggccttc
     3841 acagcatgca aaaagcattt ccgcaaaatc cacctccaaa agagggagaa cgacctgtgc
     3901 ttcgggatct ccaaaacgca cgttatactc gatcacgcgg ggaatcccgt cgggagcgac
     3961 catcaaccct gcgtaaagaa cgccgcagta gggcacgcca tcgtgacgaa gaccgtcgaa
     4021 cgtagggtcg agaatgctcc tccgcaccct ctccagaagc tcttcgccga cccagggaac
     4081 gggtgagtat gcacccatac cgcctgtatt cggtcctttg tcttcttcgt agacgcgctt
     4141 atggtcctgg ctcggaggca gaatcctgta ggttgccccg tcgctcacgg ccaaaactgt
     4201 gagttcctct cctgagagtg cgtcttcaat gataatgcgt ttgcccgcct cgccgagcgc
     4261 gcctcgtaca agcaggttct ccgccgccaa gaacgcttcc tcacgcgttt ttgttacaaa
     4321 tacgcctttt cctgcggcaa gaccatcggc cttgacaata aaaggggcgc ttcgtttggc
     4381 cagcgcttct tcaacgtctt gaagcgaagt gcattcatcc cagggagcgg tcggaactcc
     4441 ccatctgtcc atgaattttt tcgcgtggac tttgctccct tcaagaagcg cgcccgcacg
     4501 ccccgggccg aagacctcga cgccggccga gcggagttcg tcggccagac cggcgacgag
     4561 aggcgcctcc ggcccgacga ccacaagatc aatcccgtgc cgcttcacaa gaggaagtat
     4621 cgatgcgcat gctgcatcac tgacggggtg gagttttgcc aaaggcagca taccggggtt
     4681 gcccggagcc gcatggatct cccggacggt tccggagcgg gaaagcgtat gcacgagagc
     4741 atgctcccgt gcgccgcttc cgataacgag gatattcttc tttcccgtca tagcgatatc
     4801 gtctctttca tcagtggcgg aaagtacgcc atccggtaat gaacatggag atgccccgcc
     4861 gttcgactgc ggcgcatacg tctttgtccc tcacggaccc gccgggttgg atgattgcgg
     4921 cgacacccgc atctgcggcg agttcgactc cgtcggtaaa tgggaagaat gcatcggaac
     4981 cgagcacgga tcccttcgcc ttctgacctg cttgacgaac cgcgaaatca accgcgtgca
     5041 gcctgctgca gcaccctctg ccgatgccga tggcttcgcc gtttttgacg agagcgatgg
     5101 cgttgctctt tgaaatcgcg gcggccttcc acgccagaag caggtcatcc caaagatctg
     5161 gacggggtgc gcccacccat tcgccctttt cgagttccgg aacaggcggc agagaatctg
     5221 cctggacaag cagcccgctc cacgttccgg taatctggtc ggtaagcact ctgcctccct
     5281 tccatttgag cacgcgaagg gctggttttt tctccgcaag cagagcgacg gtctcctcat
     5341 cgtactccgg agcgacgagc acgtccgtaa accggtctgc cagggcaaga acggtctcca
     5401 tgtcgacttt tctggagatg ccgacgactc cgccgtatgc ggagacagga tcgcagccgt
     5461 aggcgcgttc atacgcttca aaaggagttt ccccgcaggc catgccgcac ggcgttgtat
     5521 gcttgatcac cagagctccg cagcagttct ggagaagcgc gcatccgcgc atcgcgcagt
     5581 cggcgtccag aatattgttg nnnnnnnnnn gagtgcggga agatagagcg ccgcttcctg
     5641 atgcggattc tctccatatc ggagcgtctg ttgtttctgg agtatgagcg gtattttggc
     5701 agggagagcc ggagaggcgt accctgtcgt ctccttcaga ccggaaacga tcattccgtc
     5761 ataggatgcg gttcctgcaa aggcttttat cgcaagctgt tctcttgtcg gcagcgttac
     5821 atctccttcc gaccgcagtt cttcagcgac ggtcgggtag tcctcgggtt ccataagcgc
     5881 gacgacctgc cggtagttct tcgcggcagc gcgcagcagg gaaactcccc cgatatcgat
     5941 attctccaac aggtcgtcga gcggaaggtg tttcttggcc gcctgctcga aagggtagag
     6001 gttgcagaca atcatgtcga taaggggaag atcgtgttcc tcaacatcct tcagatcgcc
     6061 gccgttctct ctccgggcaa gaatgcctcc gaaaactttg gggtggaggg tttttacccg
     6121 acctcccaag atgtggggat accccgtcac atcagcaact tccgttacca gaagccctgc
     6181 tttccgcagg tgttccgcag tccccgaact ggagagcagt tcccagccca ggtcggtcag
     6241 cgtcttcgcc agttctgtta tccccgtctt gtcatagacg gagagaagcg cgcgccgcac
     6301 tttcatcgtc gccatctcct ttcagccgga acccccgttc ggaagagttc cgaaagcgtt
     6361 ttccagtata gagcatgttc ggtcgtatgt attttttctt caaggttttc aaggctgtct
     6421 tcggggaaaa tttctaccgc ccgttgtgca aggatacggc cggaatcaat acctgcgtcg
     6481 acaaggtgaa tcgtgacgcc ggttacggcg actccgaatc tccacgcgtc gccgatgccg
     6541 tctcttccgg ggaaggacgg gaggagcgac ggatggatgt tcacgatacg cccggcatgc
     6601 gccgtaacga actcaggaga cagaattcgc atgaacccgg cgagaacgat ccattcgacg
     6661 cctcgggcgg agcaaagccc ggacagtcgg cgctccgcgt tttcctttcc ttctgaggcg
     6721 tacggaagaa caaccgtgtc gattccccgt ctgtgcgcct tctcaagtcc gggggcgtcg
     6781 ggcgcgtcgg atgcaacaaa ttcgaccctc gcatcaagcc ttccgtcgcg caccgcgtcg
     6841 gcaagcgcct ccatgttgct cccccgtcct gagaggacga tgcagaagga cgtcatgcaa
     6901 caacctctcc gataatgatt ccttgctctc tctgcgcttc aatctgctct agaaccagcg
     6961 acacatcttc ttgcgctacg atcaggcagt agccaatacc gaggttgaag acgcggcgca
     7021 tctcctcctc ttcgaccccc tcttcggaga gcaggttaaa aatagcgggc ctcggccagg
     7081 ctgtatagtc cagggagagg ccgagtccgt ccggaacgac gcgccgaagg ttatctgcaa
     7141 gcccgccgcc ggtaatgtgc gccatagcct tcacttttcc agtggagacg gcccgaaggg
     7201 ctgtgttgac gtagagcttt gtcggagcaa ggagaatttc cgcgcttcgt caaggctgta
     7261 cggcttttcc ctggttcgcc gcgctctctt ttctgaaggt atgccgtaca gcctt
//
LOCUS       DAOTIL010000024         7170 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminobacteriaceae bacterium isolate AMR_MDS_3026
            NODE_12452_length_7170_cov_3.870977, whole genome shotgun sequence.
ACCESSION   DAOTIL010000024 DAOTIL010000000
VERSION     DAOTIL010000024.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868050
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminobacteriaceae bacterium (wastewater metagenome)
  ORGANISM  Aminobacteriaceae bacterium
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae.
REFERENCE   1  (bases 1 to 7170)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7170)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:19:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,224
            CDSs (total)                      :: 3,198
            Genes (coding)                    :: 3,184
            CDSs (with protein)               :: 3,184
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7170
                     /organism="Aminobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12452_length_7170_cov_3.870977"
                     /isolate="AMR_MDS_3026"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:3034609"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..720
                     /locus_tag="P5201_00965"
     CDS             <1..720
                     /locus_tag="P5201_00965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006583803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon starvation CstA 5TM domain-containing
                     protein"
                     /protein_id="HRV97133.1"
                     /translation="HSLVGSGTTSKQLRKEKDTVLVGYGSMLLEGLVAVIAIATIMIT
                     GTIAQGGPTITYAQGFGKFAAIIGIDPKVGMSLGLLAINSFLLTSLDTATRLTRYQIQ
                     ELSNMKIDKYTATVIAVAAGMALLLTKAHGPTGNVIPAWAAIWPIFGASNQLVAALAL
                     LTIGVWVGKALKKDNRFAMYPMWFMLVTTVAALGFLIKDNMAYEHPNYILVVPSIILL
                     VLAIMMVFESLKALKNPDIKA"
     gene            1176..2918
                     /locus_tag="P5201_00970"
     CDS             1176..2918
                     /locus_tag="P5201_00970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006300611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoacid:acceptor oxidoreductase subunit alpha"
                     /protein_id="HRV97134.1"
                     /translation="MIFPFSGEVKEVSCVLSGAAGLGIQTVEDMLARIVVDSGFSVFG
                     SREYMSRVRGGNNSTELRIAPFRVDALVDRMDILVALSRNVRENIRERITPSTLIIGD
                     KEELKGELEDAGALFVDVPLSRLAAEAGGRIYSNSVAAGILLGILGLGTEKAEAFFEE
                     RFSGKKGDVAGKNTTACRSGYELGEKLVKGKGLLSGMQADLQVGKALINGSDAVSLGA
                     MAAGCNFVTAYPMSPATGVLSFFAKHAEKAGAAVEQVEDELAAINMAVGASYTGARPL
                     VTTSGGGFALMGEGLSLAGVMETPVVVHLSQRPGPATGMATRTEQADLELALYSGHGE
                     FPRAIYAPGSIESAFSLTQEAFRVALKYQTPAIVLTDQYFVNSFYNVRPDDLFFSEPP
                     SPVRETGEGYRRYEETASGCSPFGVPGWGSGIVGADSHEHDEVGHVYENFKLRKRMQE
                     KRMRKLEGMREEALAPKLYGAEEFTDLVLCWGSTIPIFKEALTLLDLKQTALLAFEQV
                     WPLHSSVDALLKRARRIVAAEGNSTAQFARLLRAQTGVAATDTVLKYNGLQFSVEEAA
                     RRLSEVFSGTGGRS"
     gene            2918..3775
                     /locus_tag="P5201_00975"
     CDS             2918..3775
                     /locus_tag="P5201_00975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005660373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent enzyme"
                     /protein_id="HRV97135.1"
                     /translation="MGTSFDLPGIEISWCPGCGDFKILDSIKQALEELGLSPLDVVVV
                     SGIGQAAKTPHYMKSHFFNGLHGRALPNATAIKAANPSLEVVAVGGDGDMYGEGGNHF
                     LHTIRRNPGITNLVFNNMVYGLTKGQASPTSPRGFRTSVQVDGVHAQPFNPLATALVQ
                     GAGFVARAYAGDVEKTKDVIKEAVQYKGYALVDIFQPCVSFNSVNTYAWFKENTYWLD
                     GHDPSDFSKALSLALSEEPFPLGVLFRGEERTTFEGSLAAYRNDKNPLFRRAAPREVV
                     KKLLDGMCR"
     gene            3789..4250
                     /locus_tag="P5201_00980"
     CDS             3789..4250
                     /locus_tag="P5201_00980"
                     /EC_number="1.11.1.24"
                     /inference="COORDINATES: protein motif:HMM:NF012787.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051920 - peroxiredoxin activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_except=(pos:3912..3914,aa:Sec)
                     /transl_table=11
                     /product="peroxiredoxin"
                     /protein_id="HRV97136.1"
                     /translation="MEGKPVPGFLLQDGSGKEFSHKDFSGSWLALFFYSKDNTSGUTN
                     EVKDFADRMSAFEELNCRIVGVSPDSVESHRKFAAKLDISFPLLSDPEHEFIEACGFW
                     KQKKMYGKEYMGVERSTLLVTPDGIVARLFSKVKVAGHAEDVLHALKERAG"
     gene            complement(4294..5031)
                     /locus_tag="P5201_00985"
     CDS             complement(4294..5031)
                     /locus_tag="P5201_00985"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRV97137.1"
                     /translation="MRDGKLLLRITLVLVLCLLARQSSAYVKILTAPGHPVSLIVEHD
                     EGRIRKAFFRSPEGVRPLEHIEQHLLLSDSLTFLCADDDILDDLVWKIDLLQPKTEKM
                     LSLWITSVTEASTAWLALAPAGAGFWESLPRAPLPREDVFLYIAPHLPAYQELDQRSG
                     PEILTFIYTMLLTKNGPKLAAIPEIYRQFLPLTALVCRAQENKDLKAAYTALHQDFER
                     MGQGGMPSREAIDNFLWKRILTVRWKR"
     gene            complement(5191..5838)
                     /locus_tag="P5201_00990"
     CDS             complement(5191..5838)
                     /locus_tag="P5201_00990"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF012521.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HRV97138.1"
                     /translation="MNEHTVRTTILLVRHGECRGNVDGLFRGRVDFPLNETGLLQAEE
                     VAGALASYRPEAVFSSPLLRALQTAESITAASSSHLFIEDGFNNICLGTWEGRPKKEI
                     AALFPEMWRTWLENPEALSVEGAESLNDVLVRSKKSLDRIVAEYQGKTVVAVTHRTVV
                     KPLLAGCLGIPSPYFWKTHMDTAAYSVLHFDDLHGYSLFCLNQTNHLSRFTTEWI"
     gene            6037..7053
                     /gene="hrcA"
                     /locus_tag="P5201_00995"
     CDS             6037..7053
                     /gene="hrcA"
                     /locus_tag="P5201_00995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013048823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_process: GO:0009408 - response to heat [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heat-inducible transcriptional repressor HrcA"
                     /protein_id="HRV97139.1"
                     /translation="MLTERQLEVVFSVVYEYIQTGEPAGSRTISKKYLRGCSPATIRN
                     EMADLEEMGYFYQPHTSAGRLPTSKAYRLYVDAIMQRRRTAPAETEKWKREIGERKQG
                     IESILTYVSRLLGQATNYVGVAALSALEEVQIQRVSFLRLGGNTVLLLIVLEGGLVHH
                     SNIQLPCELSQDMLDELGRRITTIAEGHSWSEVRDVLFRYVLDGLERVWDTCREAIVQ
                     MDALLNRRSSRLFIGGAQHILSLPDFQDIGKFQTVLSLLEQEQALAEMVERYSVKQGV
                     SVTIGDENTEEDMKQLSLVLVPASGQSRRAILGLIGPLRMDYEKSIAILETLAEDLDD
                     MLVQ"
BASE COUNT         1600 a         1778 c         2087 g         1705 t
ORIGIN      
        1 cactctctgg tcggcagcgg aactacctcc aagcagctgc ggaaagagaa ggacactgtg
       61 cttgtcggat acggctctat gcttcttgaa ggtctggtag cggttattgc tatcgcaaca
      121 atcatgatta ccggcaccat cgcgcagggc ggcccgacga tcacctatgc gcagggattc
      181 ggaaagttcg ccgcgataat cggcatcgac ccgaaggtcg gtatgtctct cggccttttg
      241 gctattaact ccttccttct gacgtcgctt gatacggcaa cccgtttgac ccggtaccaa
      301 attcaggagc tttcgaacat gaagatcgat aagtataccg ccaccgtgat tgcagttgcc
      361 gccggtatgg ctctgcttct tacgaaggct cacggcccca ctggaaacgt tattcccgcg
      421 tgggctgcca tctggccgat cttcggcgct tccaaccagc ttgttgcggc gcttgcgttg
      481 ctgacaatcg gcgtttgggt tggtaaggct ctgaagaagg acaatcgatt cgcgatgtac
      541 ccgatgtggt tcatgcttgt cacaactgtt gcggctcttg gtttcttgat caaggacaac
      601 atggcgtatg agcatcctaa ttacatcttg gttgttccct ccattatcct tttggttctg
      661 gcgatcatga tggtcttcga atctctgaag gcgcttaaaa atccagatat caaggcgtag
      721 ctgagctcta gttcaagggt tgtacagaaa cgaacgctgc aaaaataaag gaaggcgccg
      781 gttttttcgg cgccttcctt tatttggcct tttagacaga ttattttgtg cttttcccta
      841 cgctgaagga ttttgcgctc caggatttgc cccgtttcct gttttcggcg tcaattacct
      901 caaggaatct gctgccacac ctctggcatc gcatggtcga gtttttttcg tccaactcga
      961 attcgctttt gcacgataga catctaaatt tccccataaa aacacctccg tattttcttg
     1021 gaaaatcaaa gttaccctat atccatcgtc catacttcat gagatattat agcatgtggt
     1081 cgaaaagaag tggatttgct gaacgagcgc ttcgggaagt agaaagctgt ataatttaaa
     1141 acagggtaac gtactttttt gaagggagga cgactatgat ctttccattt tcgggagagg
     1201 tgaaggaagt atcctgtgtt ctctccggcg ctgctggtct gggaattcaa acagttgaag
     1261 acatgctcgc ccgtattgtc gttgattccg gtttttccgt tttcggatca cgcgagtaca
     1321 tgtccagggt aagaggaggg aacaactcga cggagctgcg tatagcgccg tttcgtgtcg
     1381 atgcgcttgt ggacaggatg gatatcctgg tggccctgag ccggaacgtg cgggagaata
     1441 ttcgagagcg tatcacgcct tccacgctga tcatcggcga taaggaggaa ctgaagggag
     1501 agcttgagga cgccggagcg ctgtttgttg atgtcccgct ttcccggctt gcagccgagg
     1561 caggaggtcg tatttactcg aattctgtag cggcgggtat tcttctggga attcttgggc
     1621 tgggaacgga gaaggcggaa gcattcttcg aggagcgttt ctccgggaag aaaggcgatg
     1681 tcgcggggaa aaatacgacg gcctgccggt cgggatacga gctcggcgag aagctggtaa
     1741 aggggaaggg gcttctctcg ggaatgcagg cggatcttca agtcgggaag gctctgatca
     1801 acggaagcga cgcagtctct ttgggggcta tggccgccgg gtgtaatttt gtgacagcct
     1861 acccgatgtc tccggccacc ggcgtgctct ccttcttcgc gaagcatgcg gagaaggctg
     1921 gcgctgccgt agagcaggta gaggacgaac tggctgcgat caacatggct gtaggcgcct
     1981 cctataccgg cgcgcgtccc ttggttacca cgtcaggagg cggctttgcc cttatgggcg
     2041 agggactgag tttggcaggt gtcatggaga ctcctgttgt agtccatctt tcgcagcggc
     2101 caggtcctgc taccgggatg gcgacgagaa cagaacaagc ggatctcgaa cttgcgctgt
     2161 actcggggca cggcgaattt ccacgggcga tctacgcgcc ggggagcatc gaaagcgctt
     2221 tttcgctgac acaagaggcg ttcagggtcg cgctgaagta ccagacgccc gccattgtgc
     2281 ttaccgatca gtattttgtg aattctttct ataatgtgcg ccccgacgac ttgtttttct
     2341 cggaaccgcc gtctcctgtc cgggagaccg gggaggggta cagacgctat gaagagaccg
     2401 catccggctg ttccccgttc ggcgttccgg ggtgggggag cggtatcgtc ggcgcggaca
     2461 gtcatgaaca cgacgaggtc ggacacgtct acgagaattt caagcttcgg aaacgaatgc
     2521 aggaaaaacg gatgagaaag cttgagggca tgcgtgaaga agcgcttgcg ccgaagctgt
     2581 acggggccga ggaatttacg gaccttgtac tctgttgggg gtcgacgatt ccgattttca
     2641 aggaggcgtt gacgctcctg gatttgaagc aaacagctct tctggctttc gaacaggttt
     2701 ggccgcttca ttcctcggta gatgcgctgt tgaaacgagc tcgtcgcatc gtcgccgcgg
     2761 agggaaacag cactgcgcag ttcgcgcgct tgctccgcgc gcagactggc gttgccgcga
     2821 cggacacggt attgaaatac aacggccttc agttctcggt tgaagaggct gcgcgcagac
     2881 tgtcggaagt tttttccgga acaggaggga gatcgtgatg ggtacgtcct tcgaccttcc
     2941 cggcatcgaa atctcctggt gcccggggtg cggtgatttc aagattctcg attctatcaa
     3001 acaggcgctc gaagagttag ggctttctcc gctggatgtt gtcgttgtct ccggaatcgg
     3061 acaggcggca aaaacgcctc actatatgaa gtcgcacttc ttcaacggac tgcatggcag
     3121 agcgcttccc aacgctacgg cgattaaggc ggcaaatccg tcgcttgaag ttgtcgccgt
     3181 agggggtgac ggcgatatgt acggagaggg cggcaatcac tttttacata cgatacgaag
     3241 aaatccgggg atcacgaacc tggtattcaa caacatggtg tacggtctga ccaaggggca
     3301 ggcttccccg accagtccga ggggttttcg tacgtccgtg caggtggacg gcgtccatgc
     3361 gcagcctttc aaccccttgg ccacggcgct tgttcaggga gccggtttcg tcgcaagggc
     3421 ctatgcgggc gacgtcgaaa agacgaagga cgtcatcaag gaagccgttc agtacaaggg
     3481 gtatgccctt gtcgatatct tccagccgtg cgtctccttc aattcagtga acacctacgc
     3541 gtggttcaag gaaaacacat actggctgga cgggcacgat ccttcggatt tctcgaaggc
     3601 tctctcgctt gccctgtccg aggagccttt tccgctggga gttctttttc gcggagaaga
     3661 acggacgacc ttcgaggggt cgctggccgc atacaggaac gacaaaaatc cgctcttccg
     3721 ccgcgctgcg ccgcgagagg tcgtgaagaa gttactcgac ggaatgtgcc gttaaggagg
     3781 gaaaaagaat ggaagggaag cccgtgccag gttttctcct tcaggatgga tcagggaagg
     3841 agttttcgca taaagatttt tcagggagct ggctggcgct ctttttttac agcaaggaca
     3901 atacgtcggg gtgaacaaac gaagtcaagg actttgcgga caggatgtcc gcttttgaag
     3961 agctgaactg ccgaattgtt ggtgtgagcc ctgattcggt cgaaagtcac cgcaagtttg
     4021 ctgcaaaact tgacatatcc tttccccttt tgagcgatcc tgagcatgag ttcattgaag
     4081 catgcggttt ctggaagcag aagaagatgt acgggaagga gtatatggga gtcgaacgtt
     4141 ctacgttgct tgtcacccct gacgggatcg ttgcccgtct cttctcaaag gtgaaagtag
     4201 ccggtcacgc agaagacgtt cttcatgccc tgaaagaacg tgcagggtaa aaaacggggc
     4261 gctgccgaaa cggggcgccc cgcacttctt ccgtcatcgt ttccatcgaa cagtaagaat
     4321 gcgtttccac agaaagttat ctatggcctc ccgtgaaggc attcctcctt gccccatccg
     4381 ctcaaagtcc tgatgcaagg ccgtgtaggc cgctttgagg tccttgtttt cctgtgcccg
     4441 gcagacaaga gcggtcaggg gaagaaactg tctgtatatt tccgggatgg ccgccagttt
     4501 cgggccgttt ttcgtgagga gcatcgtata aatgaacgtc aggatttccg gtccgcttcg
     4561 ctggtcgagc tcttgatacg cagggagatg cggcgcgatg tagagaaaaa cgtcttccct
     4621 ggggagcggc gcccgaggaa gcgattccca aaagccggcg cctgccggcg ccagtgcaag
     4681 ccaggcggta gatgcttcgg tgacggatgt tatccagaga gagagcatct tttcggtctt
     4741 cggttgcagg aggtcgattt tccacaccaa gtcgtcaagg atgtcgtcgt ctgcgcacag
     4801 aaaagtaagc gaatccgaaa gaagaagatg ctgttcgatg tgttccaagg gacgtactcc
     4861 ctccggcgaa cggaagaacg ctttccggat acgcccctcg tcatgttcga caatcaaaga
     4921 tacggggtgt ccgggggccg tcagtatctt tacgtaagcg gaactctgcc gtgcaaggag
     4981 gcagaggacg agtacaagtg tgatgcgaag caaaagtttt ccgtctctca agaaggcagc
     5041 ctcccggatg aaaacatgag tccgaatgaa gcgccggagc acttgcagtc gggttttgcg
     5101 gttcttcttc tccgtatcgt ataatactat atttttcgaa aaaggtggag tatagctggt
     5161 cttttctttt caatgccgcc gtttcccgcc ttaaatccac tcggtagtaa aacgtgaaag
     5221 atgattcgtt tggtttaaac aaaagaggct atacccgtga agatcgtcga aatggagcac
     5281 tgagtaggca gcggtatcca tatgggtttt ccagaagtag ggagaaggaa taccgaggca
     5341 tcctgcgaga agcggtttga caacagttct gtgtgtgact gcgacgacgg ttttcccttg
     5401 gtattcggca actattcggt caagactttt ttttgagcgc acaaggacat cgttcaaaga
     5461 ttccgccccc tcgactgaaa gcgcttccgg gttttcaagc caggtcctcc acatctcagg
     5521 gaatagtgcg gctatctctt tcttgggacg cccttcccaa gtcccgagac agatgttgtt
     5581 gaacccatcc tctataaaca agtgagaaga tgacgccgcg gttattgatt cggcggtctg
     5641 caaggctctg agaagaggac tcgaaaagac tgcttccggc cgataagaag cgagggcgcc
     5701 ggcaacttct tcggcttgca ggagtccggt ttcgttcaaa ggaaaatcaa cccttccccg
     5761 aaaaagcccg tcaacatttc ctcggcactc cccgtgacgc acgagtaaga tggtggtgcg
     5821 aacggtgtgt tcgttcattt ttccctccgt ctccgcagtc tcgtggagtc gacgacggaa
     5881 gagagcttca tcgtggatct gccgcgatag cgggaaaaaa aataaaagcc tttgaccagt
     5941 cttgacaatc atatcatgga ttgttactct accttccggg agcgatgcat tggcactcac
     6001 attgaaagag tgctaacgca acgaggaggt gtcgaaatgc ttacggagag gcagctcgaa
     6061 gttgtttttt ccgttgtcta cgagtatatc cagaccggag agcccgcggg ctcccggacg
     6121 atttcgaaga agtatctccg ggggtgcagt ccagcgacaa ttcgaaatga aatggccgac
     6181 ctggaggaga tggggtattt ctaccagccg catacgtccg ccggaaggct gcccacgtcg
     6241 aaggcctatc gcctgtatgt ggatgcgatc atgcagcgaa ggcggaccgc tcccgccgag
     6301 acggagaagt ggaagcggga gatcggagaa cgaaaacagg ggatcgaatc gatcctcacc
     6361 tatgtttccc ggctcctcgg gcaggcgacg aactatgtgg gggtagcggc tctctctgcg
     6421 cttgaagagg tacagatcca acgggtgagc ttccttcgcc ttggaggcaa tactgtgctg
     6481 cttctgattg ttcttgaagg cggactggtg catcactcca acatccagct tccctgcgag
     6541 ctttctcagg acatgcttga cgagcttgga cgccgtatca ccaccatagc cgagggccat
     6601 tcttggagcg aagtccgcga tgttcttttc agatatgttc tcgacggtct ggagcgggtc
     6661 tgggatacct gccgcgaggc aatagtccag atggacgcgc tcttgaacag gcggagttcc
     6721 cgcctgttca tagggggcgc gcagcatatc ctgagcctgc ccgatttcca ggatataggg
     6781 aagtttcaga cggtgctctc tcttcttgaa caggaacagg cccttgcaga gatggtcgaa
     6841 cgctatagtg taaaacaggg ggtctcggtt accatcggag atgaaaacac ggaagaggac
     6901 atgaagcagc tctctcttgt tctcgtgccg gcatccggcc agagccgcag ggcgatcctg
     6961 gggctgatag gcccgctccg tatggactac gagaagtcga ttgccatact ggaaaccctt
     7021 gctgaagatt tggacgatat gctcgtccag tagttttata tgaaaggagc gacacgaatg
     7081 agcagtgtta acgatcaggt ttcttcggag ctggaaaaag agggggtcgc ctccgcagaa
     7141 acctgatcgt taacactgct catacgtgtc
//
LOCUS       DAOTIL010000025         7142 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminobacteriaceae bacterium isolate AMR_MDS_3026
            NODE_12528_length_7142_cov_4.051644, whole genome shotgun sequence.
ACCESSION   DAOTIL010000025 DAOTIL010000000
VERSION     DAOTIL010000025.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868050
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminobacteriaceae bacterium (wastewater metagenome)
  ORGANISM  Aminobacteriaceae bacterium
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae.
REFERENCE   1  (bases 1 to 7142)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7142)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:19:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,224
            CDSs (total)                      :: 3,198
            Genes (coding)                    :: 3,184
            CDSs (with protein)               :: 3,184
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7142
                     /organism="Aminobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12528_length_7142_cov_4.051644"
                     /isolate="AMR_MDS_3026"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:3034609"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            45..119
                     /locus_tag="P5201_01000"
     tRNA            45..119
                     /locus_tag="P5201_01000"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:77..79,aa:Val,seq:gac)
     gene            390..1385
                     /locus_tag="P5201_01005"
     CDS             390..1385
                     /locus_tag="P5201_01005"
                     /inference="COORDINATES: protein motif:HMM:NF014647.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BMP family ABC transporter substrate-binding
                     protein"
                     /protein_id="HRV97140.1"
                     /translation="MKALRVLFALIVLLVFALSSGASQLSIAMITDVGGVNDQSFNQS
                     AWEGLQRAEKDLGVKAAYIESRQDADYGPNLETLLDAGTDLIWGIGFKMADAVLEAST
                     NNVDQKYAIIDFAWEKTPENLIGVVFKAEEASFLVGYIAAKMTKTGKVGFIGGMSFPV
                     IWGFEYGFRAGVAQAGKNVEILSQYAESFTDAAKGKSIANSMYQQGADIIFHASGGVG
                     DGLIEAAKEQGKWAIGVDRDQNYLAPDNVLTSAMKRVDNAIFNIAKELIEGKWQGGHT
                     VSYGLAEGGVDIAPTTSKHVPAELLAEVEAVKARIIAGEVVVPFSKETFASFEVK"
     gene            1454..2998
                     /locus_tag="P5201_01010"
     CDS             1454..2998
                     /locus_tag="P5201_01010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006301377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HRV97141.1"
                     /translation="MPERKKNAAELAVEMKQITKRFGNFVANDSIDFDVRRGEVHALL
                     GENGAGKSTLMNQLYGLYLPSSGEIFINGKKVRITGPKIAIARGIGMVHQHFMLVQPF
                     TVAENIVLGCESVRAFGILDTARAFREVEDLSRRYGLAVDPGARIQDISVAMQQRVEI
                     LKCLYRGAEILILDEPTAVLTPQEIDFLAEILQNLTAEGKTVIIITHKLREIKKMAHR
                     CTVIRRGVKIGTVDVASRSESELAEMMVGRAVSFDVEKTPARVQEPVLLLTDIYALNN
                     RGLPALKGISLVVKRGEILALAGIDGNGQSELVEVLTGLRPVESGSIRFRNTEITDFP
                     SGKRIDMGLAAIPEDRQKRGLIGPFTIAENLILEERSLPPFSRSGILDLQAISRHAKT
                     LIDRFDIRPPREQYLATSLSGGNQQKVILAREITRDPELLIASQPTRGLDVGAIEYVR
                     KRLVQVRDAGKAVLLISLDLDETLALADRIAVIYEGRIVAVLDREETDERTLGLLMAG
                     GGAVHV"
     gene            2991..4130
                     /locus_tag="P5201_01015"
     CDS             2991..4130
                     /locus_tag="P5201_01015"
                     /inference="COORDINATES: protein motif:HMM:NF014684.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HRV97142.1"
                     /translation="MSDEKRKLLAASPLFFTFFSVLTGLLVGALVLLISGYSPLKAYH
                     VIIDGIFSRPSYIVYTVIYSTPLIMTGLSVAFAFRTGLFNIGAEGQFIMGALGAALAG
                     AFLHLPPVLHAIVAALAGIAAAAVWGGIAGFLKARFGVNEVIATIMLNWTALYLNNFI
                     VTLPSIKRTATNATAFILDSARIEILPLWKVSEAGRTWRAMHPFWHDIFRTPFNAGIL
                     VALFLAFFCWWILNRTTLGYELRAVGHNADCSEHTGINVKARMISSMAIAGGLAGAAG
                     ALHVLGWTRQAAELAAMEGLGFDGIAVALIGGTSPIGCIPAGLLFGALKYGGSKLQFA
                     LRAPIEVVNIVIGSIIFFVAMPKLLRIIRRWLEPKKRQGGDSHAA"
     gene            4120..5061
                     /locus_tag="P5201_01020"
     CDS             4120..5061
                     /locus_tag="P5201_01020"
                     /inference="COORDINATES: protein motif:HMM:NF014684.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HRV97143.1"
                     /translation="MLLESIAFIIATTLMYSAPLIFTALGGVVSENAGVVNIGLEGMM
                     TLGAFVGAAVGYFANDAWIGFLAAGAAGGALAFFHALACVTFLADHIVSGIAINFIGP
                     GLSLFLSRYFFDGATQTLSLSLDAKIPRPLNGVFEQNGFFDMVFNQYATVYLALFLVA
                     FLWFILYRTRLGLRIRAVGEHPKAADTLGINVLRIKYLAVITSGVLAGFGGAALSVAI
                     VSRFSSTLVSGQGFIALAAMIFGKWKPVGTLAACLLFSSAQGLTVYLGRGDLQRMLPI
                     PSQLLATIPFLLTLAVLIGFVGKSRPPAADGIPYEKE"
     gene            complement(5131..5856)
                     /locus_tag="P5201_01025"
     CDS             complement(5131..5856)
                     /locus_tag="P5201_01025"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRV97144.1"
                     /translation="MGKKGILFLVWFLGFVSAVEGAATWYNAYTGGMWNNPGSALIDT
                     MLYNRMMAETSLKKMQRTPSGGVGASGQVRTLYRPSSSQSSIPAKVAAVLSNDNATKK
                     ELTVFFKEGLQSFEQFAQQAGRPYDLGFSMAFFAGVCIGLTTENEVDDDAILAAASQM
                     DKLLAEQSGIAKASDTQRREFAEVLACMAVFALAGHTQAEEAGDDEAVESFKQFARQS
                     MIEILGMDTNRLRMDGRGIRIIQ"
     gene            6199..6789
                     /locus_tag="P5201_01030"
     CDS             6199..6789
                     /locus_tag="P5201_01030"
                     /inference="COORDINATES: protein motif:HMM:NF012708.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051219 - phosphoprotein binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FHA domain-containing protein"
                     /protein_id="HRV97145.1"
                     /translation="MTIKRCPNGHFYDPDANNSCPWCAVPADDNERTRRINPAAIPVA
                     GNAGSTVKLSPRNTPSSGSSIDEGHTVAVVKRKTGIDPVVGWLVCVKGPDRGRDYRIR
                     SEKNSVGRADSMDICISGDDTISRNDHAFVVYDPRKKIFRIQAGLSRGLVYLNGEEVI
                     ASELLAAYDTISVGQSELLFIPLCGERFQWETEQES"
     gene            6765..>7142
                     /locus_tag="P5201_01035"
     CDS             6765..>7142
                     /locus_tag="P5201_01035"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HRV97146.1"
                     /translation="MGNRTGILNRILSAVCLLLLLAGAALAAPSTLRVRQAYLDYPSL
                     TVYADLLDASGKPFVPGGAEQLTATVGGRAFDVSRWSAFSAKNDGVLIIFLVDTSASI
                     GQNRFTELQETVSGWIRRMGPSDR"
BASE COUNT         1602 a         2015 c         1863 g         1662 t
ORIGIN      
        1 ctccttgaca aagacgacaa cggtctgtag aatatctcct cgttgggtgg ttagttcaga
       61 ggaagaacgc ttccttgacg cggaagaggt cagaggttcg agtcctctac cacccaccat
      121 tgactactaa agagcttcag ctatctgctg gagctctttt tgtatttgaa agcagtctat
      181 attctcttac acactgcctc cttcacacaa agacaatctg ctctttacag tatcctcttt
      241 tcatcaaaac tgattatccc attgaaaaca cgcttcgaga gccgcggatg gcgcttgcgg
      301 aaatttttcc agggatgtaa gattgttgaa atattgaagc cggtttttct tccggcacgt
      361 aatactatcc caagagagga gttggtctaa tgaaagcttt acgggtgctg ttcgctttga
      421 ttgtccttct tgtttttgcg ctgtcttcgg gggcatcaca actatcgatt gcaatgatca
      481 cagacgtagg gggcgtgaac gatcaatcct tcaaccaatc agcatgggaa gggcttcaaa
      541 gagctgaaaa agatctgggt gtgaaggcag cctatataga atcgcgtcaa gacgccgact
      601 acggcccaaa ccttgaaacc ctgctcgacg caggaacgga cctgatctgg ggaattggtt
      661 tcaaaatggc cgacgcagta ctggaagctt ccacgaacaa cgtcgatcag aaatacgcca
      721 ttatcgactt cgcctgggaa aaaacaccgg aaaaccttat cggcgttgtc ttcaaagctg
      781 aagaagcatc cttcctcgtc ggttacatag cggcgaagat gacgaagaca ggaaaggtcg
      841 gcttcattgg cggcatgtca ttccctgtga tctggggatt cgaatacggg ttccgcgcag
      901 gtgttgcgca agctggaaag aacgtcgaaa tcctgagcca gtacgcagaa tccttcactg
      961 acgctgcaaa gggcaaatcc atcgccaaca gcatgtacca gcagggcgcc gacatcatct
     1021 tccatgcctc agggggcgtc ggcgacggtc tgatcgaagc ggctaaggaa caggggaagt
     1081 gggcgattgg cgttgacaga gaccaaaact atcttgctcc cgacaatgtt ctcaccagcg
     1141 ccatgaaacg cgttgacaac gcgatattca acatcgcaaa ggaactgatc gagggtaaat
     1201 ggcaaggcgg gcacacggtt tcttacggac tcgcggaggg cggagtagat attgccccca
     1261 caacatcaaa acacgttcca gcggagctgc tcgcggaagt ggaagctgtt aaagcgcgaa
     1321 tcatcgccgg agaagttgtc gtcccattca gcaaagagac ctttgcttcc ttcgaggtga
     1381 aatagaaacg agcgcggggg aagtacataa aaaactcctc cgcgcacctt ttctattctg
     1441 aggaggtggg gttgtgccgg aacgtaaaaa gaacgctgcc gaactggcgg tagagatgaa
     1501 acaaattaca aagcgattcg gaaacttcgt cgcaaacgac tcaatcgatt tcgatgttcg
     1561 ccgcggcgag gtccacgcgc tcctcggtga aaacggcgcg ggaaaaagca cgctgatgaa
     1621 ccagctctac ggcctttatc ttccttcttc gggagagatt ttcatcaatg gtaaaaaagt
     1681 ccggataacc ggccccaaga tagccatcgc caggggaata ggaatggtgc atcagcattt
     1741 catgcttgtg cagccgttta ccgtagccga gaacatcgtt ctcggctgcg aatcggttcg
     1801 agcattcggc atccttgata ccgcacgtgc gttccgagag gtggaagacc tttcgcgccg
     1861 ttacggactt gctgtcgatc ctggcgcacg aattcaggat atttctgtgg ctatgcagca
     1921 gcgcgtggaa attttgaagt gcctctaccg tggggcagaa atcctcatac tcgacgaacc
     1981 gacggcagtt cttactcccc aggagattga ttttcttgcc gagatactcc aaaatctgac
     2041 tgctgagggg aaaacagtca tcattatcac ccataaacta cgagagataa aaaaaatggc
     2101 gcatcgctgc accgttatca ggaggggcgt aaagataggg actgtcgacg ttgcgtcccg
     2161 ctccgagagc gagctggccg aaatgatggt cggacgcgcc gtttctttcg atgtcgagaa
     2221 aacgcctgca agagtgcaag agcctgtgct cttgcttact gatatttatg ccctcaataa
     2281 caggggactt cctgcgctca aaggtatatc gctcgtagta aaacgaggtg agattctggc
     2341 tcttgcagga atcgacggaa acgggcagag cgagcttgta gaggtcttga ccggcctccg
     2401 ccccgtcgaa tccggctcca tccgtttccg caacactgag atcacggatt tcccatccgg
     2461 aaagagaatc gacatgggct tggcggcaat cccggaggat cgccaaaaac gcggcctcat
     2521 cggccccttc acgatcgccg agaaccttat ccttgaggag cgctcactcc cgcctttttc
     2581 gcgcagcggt attcttgatc tgcaagcgat cagccgtcat gccaaaacgc tcattgatcg
     2641 tttcgatatt cgccctcccc gcgaacagta tctcgctacg tcgctctccg gaggaaacca
     2701 gcagaaggtc atcctcgcaa gggaaatcac acgcgatccg gagctgctta tcgcgtcgca
     2761 gccgacaagg ggacttgacg tgggcgccat tgaatacgta cgcaaaagac ttgtccaggt
     2821 tcgcgacgca gggaaagctg ttctccttat ctctctcgat ctcgacgaaa cgcttgctct
     2881 cgccgaccgt atcgcagtca tctacgaagg ccgcatcgtt gccgttcttg accgggaaga
     2941 gacagacgag cgcacgctcg ggcttctcat ggcaggaggt ggagcagtgc atgtctgacg
     3001 aaaaaaggaa gctacttgca gcttcgcctc tcttcttcac ttttttttca gtgctcacag
     3061 ggcttcttgt cggcgccctc gtgcttctga tcagcggata cagccctctc aaggcgtatc
     3121 atgtgatcat cgatgggatt ttctccagac cgagctatat cgtctacaca gtcatttact
     3181 ccacgccgct catcatgacg gggctctcag tcgctttcgc tttccggaca ggtcttttca
     3241 atatcggggc ggagggacag ttcatcatgg gcgccttggg cgcggcactc gcaggcgctt
     3301 ttctacacct gcctcctgtg ctccacgcca tcgtcgccgc actggcgggc atcgcagcgg
     3361 ctgccgtctg gggaggcatc gcgggctttc ttaaagcgcg cttcggcgtc aacgaagtca
     3421 tcgccacaat tatgctcaac tggacagccc tctacctgaa caactttatt gttacgctgc
     3481 cgtcaatcaa acgaactgca acaaatgcga cggcattcat tctggactcg gcgcgaatcg
     3541 aaatactgcc gctgtggaaa gtaagcgaag cgggaagaac ctggcgcgct atgcatccat
     3601 tctggcacga cattttcaga acacccttca atgccggaat actggtcgcg ctcttccttg
     3661 cttttttctg ctggtggatc ctgaacagga cgacgctcgg ttacgagctt cgggccgtcg
     3721 gtcacaacgc ggactgttca gagcataccg gcatcaatgt gaaggcccgc atgatctcct
     3781 cgatggccat cgccggagga cttgcaggag cggcaggagc gctccatgtc ctcggctgga
     3841 cgcgccaggc cgcagaatta gcagcgatgg aaggtctggg cttcgacggt atcgcggtcg
     3901 ctctgatcgg cggcacctcg cctattgggt gcattccggc gggacttctg ttcggcgccc
     3961 tcaaatacgg aggatccaag cttcagttcg ccctcagagc gcctattgaa gtggtgaaca
     4021 tcgtcatcgg ctctatcatc tttttcgtcg ccatgcccaa gctgctccgg atcattcgac
     4081 ggtggctgga accaaagaaa aggcagggag gtgattccca tgctgcttga gagcattgcg
     4141 ttcattatcg ccacgacgct catgtactcg gccccgctta tcttcaccgc cctcggcggc
     4201 gtagtttcgg agaacgcggg cgtggtcaac atcgggcttg aaggaatgat gaccctcggc
     4261 gccttcgtcg gagcggccgt cggctacttc gccaacgatg cgtggatcgg ttttctcgct
     4321 gccggagcgg ccggaggagc gttggcgttc ttccacgccc tcgcatgcgt gacgttcctc
     4381 gccgaccaca ttgtctcggg tatcgcgatc aacttcatcg gccccggtct ctcccttttt
     4441 ctctctcgct acttcttcga cggggcgacg cagacgctct cactctctct cgacgcgaaa
     4501 attcctcggc cgttgaacgg ggttttcgag cagaacggct tctttgatat ggtcttcaat
     4561 cagtacgcaa ccgtctacct tgctctcttc ctggtcgcct ttctctggtt cattctctat
     4621 agaacccgtc tcggcctgag aattcgcgcc gtcggcgagc accccaaagc ggcggatacc
     4681 ttgggtataa atgttctcag aatcaaatac cttgctgtta tcacttccgg agttcttgca
     4741 ggattcggag gggccgcgct cagcgtggct atcgtctctc gcttcagctc gacgctcgtg
     4801 agcggtcagg ggttcatcgc cctcgcggcg atgatcttcg gaaagtggaa acccgtcgga
     4861 acgctcgcgg catgccttct cttcagctcg gcccaggggc tcaccgttta cctgggacgt
     4921 ggggacctcc agaggatgct ccctataccc tcccagcttc tggcgacaat tccgttcctg
     4981 ctcacgcttg ccgtgctgat aggattcgtc ggcaagtccc gccctccggc ggcggacggc
     5041 atcccttacg agaaagaata acgaaaagag cccgtcgaaa agtttaatat gttccgccgg
     5101 gctctttttt ctagtttcat tgatggtcta ttactggata atgcgtatac cccgaccgtc
     5161 catacgaaga cggtttgtat ccattcccag aatctcgatc atcgactgac gggcaaactg
     5221 cttgaacgat tctacagcct cgtcgtcgcc tgcctcctcg gcctgcgtat gaccggcaag
     5281 agcaaaaaca gccatgcacg ccaggacctc tgcgaattcc cttcgctgcg tatcggaggc
     5341 tttggctatc cccgactgtt ccgcaagcag cttgtccatc tgcgacgcgg ctgctagaat
     5401 ggcgtcatcg tccacttcat tttccgttgt taacccgatg cagactccgg caaagaacgc
     5461 catcgaaaag cccagatcat acggacggcc cgcctgttga gcaaactgtt caaaactctg
     5521 caacccttct ttgaaaaata cagtcagttc cttctttgta gcgttatcgt tgctcaaaac
     5581 agcggcaacc ttggcgggta tggaggactg agaggaagaa ggccggtaga gcgtccgcac
     5641 ctgccccgaa gctcccactc caccggaagg cgtcctttgc atcttcttca acgaggtttc
     5701 tgccatcatt ctgttataga gcatcgtgtc aattaacgcg gagccggggt tgttccacat
     5761 tccccccgta taggcgttgt accatgtagc ggctccttca accgcggaaa caaaacccaa
     5821 aaaccatacg agaaacagaa ttcctttttt ccccattccg tatcgccccc ttcgaaaaag
     5881 agtcgctgcc cgtacgaggt ccctcctttt tacgcatatt ctactcgttg cagtttcaca
     5941 tgagcaagag ttcttttcat aaaggtgaaa agggcttttt gccttgattg gatgccgaag
     6001 aagctgttga aaaaagtgtt tttttggcgt atggtactct tgtttataat atacgcctcc
     6061 gaatgccctt tcgcattttt ttaggtatcc agaatacatt tttgacgcta ccctttccga
     6121 atgagcggaa aaagtggtga attgtcgatt tctgcttctg tgggatatca tctcaattct
     6181 gaaacggagt gattcgaaat gacgatcaaa cgatgcccga acggacactt ctacgatccc
     6241 gacgccaata attcttgtcc gtggtgcgct gttcccgccg acgacaacga aagaacgcgg
     6301 cgaatcaacc ccgcagcgat accggtggcg ggcaacgcag ggtctaccgt aaaactgtct
     6361 ccccgaaaca caccttcgtc cggaagctcc atcgacgaag ggcataccgt cgccgtcgtc
     6421 aagcgcaaga cgggaatcga tccggtcgtc ggctggcttg tctgcgtcaa ggggccggac
     6481 cgcggacgcg actaccgaat acgttcggag aagaactctg tcggcagggc cgactccatg
     6541 gacatctgca tctccggaga cgatacaatc tcgcggaacg accatgcatt cgtcgtctac
     6601 gaccccagaa agaagatctt cagaatccag gcgggattga gcagagggct ggtctatctc
     6661 aatggagaag aggtcattgc ctcggagctg ctcgccgcct atgacacaat ctcggtcggg
     6721 cagagcgaac ttcttttcat tcctttgtgc ggggagcgct tccaatggga aaccgaacag
     6781 gaatcctgaa ccgcatcctc tccgcagtct gcttgcttct tttgcttgca ggcgccgcgc
     6841 tcgccgcacc ctcgacattg agagtccgcc aggcgtacct cgactatcct tctttaaccg
     6901 tgtacgccga ccttcttgac gcctccggaa aaccttttgt ccccggcgga gccgagcaac
     6961 ttaccgcgac cgtaggcgga cgcgccttcg atgtgagccg ctggagcgct ttttccgcga
     7021 agaacgacgg agtccttatc atcttcctcg tcgatacctc cgcctcaata ggacagaacc
     7081 gcttcaccga actgcaggag acggtctccg gatggatccg ccgtatgggc ccctccgacc
     7141 gc
//
LOCUS       DAOTIL010000026         7089 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminobacteriaceae bacterium isolate AMR_MDS_3026
            NODE_12630_length_7089_cov_3.818595, whole genome shotgun sequence.
ACCESSION   DAOTIL010000026 DAOTIL010000000
VERSION     DAOTIL010000026.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868050
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminobacteriaceae bacterium (wastewater metagenome)
  ORGANISM  Aminobacteriaceae bacterium
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae.
REFERENCE   1  (bases 1 to 7089)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7089)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:19:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,224
            CDSs (total)                      :: 3,198
            Genes (coding)                    :: 3,184
            CDSs (with protein)               :: 3,184
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7089
                     /organism="Aminobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12630_length_7089_cov_3.818595"
                     /isolate="AMR_MDS_3026"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:3034609"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..388
                     /locus_tag="P5201_01040"
     CDS             <1..388
                     /locus_tag="P5201_01040"
                     /inference="COORDINATES: protein motif:HMM:NF024118.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HRV97147.1"
                     /translation="HATQLLGIPVEAVPAYNIVHKRDTGQPFHPKGEHNGYILTFGDT
                     RIYIAGDTENTPEMKALQDIDAAFLPMNLPFTMTPEMVADAALAFKPKALYPYHMGQM
                     DEERPAKLAELLKGSGIDLRIRKMGR"
     gene            complement(449..1525)
                     /locus_tag="P5201_01045"
     CDS             complement(449..1525)
                     /locus_tag="P5201_01045"
                     /EC_number="5.1.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF013431.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016857 - racemase and epimerase activity,
                     acting on carbohydrates and derivatives [Evidence IEA];
                     GO_function: GO:0030246 - carbohydrate binding [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldose epimerase family protein"
                     /protein_id="HRV97148.1"
                     /translation="MSEHCSSAPSADSVLFGETRTGEKVFLHTARNGSLALRCIDFGA
                     AVTSLEVPDRDGVCENVVLSYDRLAGYEEDTCWCGLLCGPVAGRIAGASFSLEGRPCE
                     LEANDGTSCLHSGPEGFSYSVWHGETFSSPGEAGVRFRFSTLPGQWGFPGTLDVTATY
                     TLTGNSLILAWEAETAVPTLLAPAFHGYFNLGGENTASIADHRLAIDADRFMPLDEQH
                     LPCMPRSVDGTPFDFRAPRSPEHSQWASDRQIQLGNGYDHPFLLNSPENGRTPDIVLE
                     YPASGRIMEVFTDQPACVLYTGGSLNSSQRFSGDRGGAPGLALALETQWYPNTPALPD
                     LPQPVLFPGEVFRAKTKWRFIAAK"
     gene            complement(1602..2768)
                     /gene="gguB"
                     /locus_tag="P5201_01050"
     CDS             complement(1602..2768)
                     /gene="gguB"
                     /locus_tag="P5201_01050"
                     /inference="COORDINATES: protein motif:HMM:NF040906.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HRV97149.1"
                     /translation="MRKLKEMFMMNIRQYAMMLALLFIVLLFQYLTDGVLLRPLNLTN
                     IIQQNGYILILAIGMLLCILTGNIDLSVGSVAAFVGAVGGTLMTVWKWPVIPSILACL
                     VLGILIGCWQGYWIAYFRIPAFIVTLAGMLCFRGLTLVMLKGQTLAPMPDLFRKLFTT
                     FIPDVLGGEGLNYTALTIVAILLCAFAFFELRDRQNKKAYGFEVAPFPFWVMKILLVA
                     AVVTAFTYQLARYRGIPMVLVLLGALVLIYSFITRKTVFGRHVYAFGGNEKAAKLSGI
                     DTNKVFFLVYANMGLMAAVAGLAFMGRLNAASPVAGVNFELDAIGACFIGGASASGGI
                     GTVTGAIIGGLIMGVLNNGMSIVGVSIDWQQVVKGLVLLAAVAFDVYTKKKTAK"
     gene            complement(2768..4312)
                     /gene="gguA"
                     /locus_tag="P5201_01055"
     CDS             complement(2768..4312)
                     /gene="gguA"
                     /locus_tag="P5201_01055"
                     /inference="COORDINATES: protein motif:HMM:NF040905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter ATP-binding protein"
                     /protein_id="HRV97150.1"
                     /translation="MAKTLLRMKSITKTFPGVRALNDVCFDVEEGEIHALVGENGAGK
                     STLMNILSGVYPFGSYEGEIVFDGETCVFNTIKDSEKIGIVIIHQELALVPYLSIAEN
                     VFLGNERARRGVIDWSETYARTDDLLKQIRLHENPATITKDLGVGKQQLVEIAKAISK
                     DVRLLILDEPTAALNDEDSNNLLNLLLDLKKKGITSIIISHKLNEVEKIADRITVIRD
                     GAVIETLDNATKSIREDRIIKGMVGREIVDRFPKRPPHARGDLALEVRDWTVGDPLFF
                     DRKKIKGVSFNVHKGEVVGLVGLMGAGRTELAMSLFGRSYGSDFSGCLFLGGQSATLH
                     SPKEAIARGMAYVTEDRKSAGLILIDDIRHNISLASLGKLSDKRGVLDENKEITVAED
                     LRKKMSIKTPSVFQKVGNLSGGNQQKTILARWLFANPDVLLLDEPTRGIDVGAKYEIY
                     CIINELAAMGKAIVLISSEMPEVLGMSDRIYVMNEGRFVGELDRESASQEAIMKCIMQ
                     SNREVA"
     gene            complement(4401..5453)
                     /gene="chvE"
                     /locus_tag="P5201_01060"
     CDS             complement(4401..5453)
                     /gene="chvE"
                     /locus_tag="P5201_01060"
                     /inference="COORDINATES: protein motif:HMM:NF040907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter substrate-binding protein"
                     /protein_id="HRV97151.1"
                     /translation="MKKMLLALFALVLLFSATAAFAADVLVGVAMPTQSLQRWNEDGA
                     NMKKILEGKGYKVDLQYAENNVSVQISQLENMITKGVKVMIIASIDGSALSDVLAKAA
                     EAKIPVIAYDRLIRQSPNVDYYATFDNFKVGVIQGKFIEEKLGLKDGKGPFNIEFFGG
                     SPDDNNAFFFNGGAMSILQPYLDSGKLVCHSGQTKMEQIGILAWKSEDAQARMDNLIS
                     RSYTDKKLDAVLSPNDSLAYGISASLENNGYIPGKDWPIITGQDCDRANVKNMIAGKQ
                     SMSVFKDTRALATKVAEMVDDIIQGKQPEVNDTKTYDNGMKVVPSYLLEPVFADVNNY
                     KELLLDSGYYKEEDLK"
     gene            6067..>7089
                     /locus_tag="P5201_01065"
     CDS             6067..>7089
                     /locus_tag="P5201_01065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRV97152.1"
                     /translation="MLTGKRVIVCCALAAGLLLRLPFAGGAEETAGPTAMEEGEAVFQ
                     LSSGDVSAASVLSQSPAVEEAAGPLQTAEKTSEEGIPDAPSAETAGNELGATSADVPV
                     SAGSEQLARKLFEIEVASRVAEEELGKLPSLVQSERTKIANELNQWANTDLRRIIDQH
                     YLEVEKALAETTLPEDHSSVGIWWKQVKAGVFLNEEIEEVVALFVKRAGPRLSTVHEE
                     FQRTLLESLEQRSQTLLRRSMEEIRTPFVATVKDRLPLYNAIPVPNTERAALSIALSG
                     EQSDRGRLSSGMAVIGGVVLTVLGRKLVQKMMHLAVVKIGGKVLSRMIPIIGVGLLLY
                     EGIQMGQ"
BASE COUNT         1440 a         1968 c         2214 g         1467 t
ORIGIN      
        1 ccacgcaaca cagctgttgg gaattccggt cgaagctgtt cccgcctata acatcgtcca
       61 caagagagac accgggcagc cgttccaccc gaagggcgag cacaacgggt acatcctcac
      121 gttcggggat acccggatct acatcgccgg agacactgaa aacacgcccg agatgaaagc
      181 gctgcaggat atcgacgccg cttttcttcc gatgaacctt cccttcacca tgacgccgga
      241 gatggttgcc gacgctgccc tcgccttcaa accgaaggcg ctctacccct accatatggg
      301 gcagatggac gaggagcgtc cggcgaagct ggcggaactg ctcaagggaa gcggtatcga
      361 cctgaggatc cgaaagatgg ggcggtagcg gaaaagaatc cggactgtcg gcaaggcggg
      421 ggggcggcgc ctctccgcct tgcgttcttt atttggccgc aatgaaccgc cacttcgtct
      481 ttgcacggaa aacctctccc gggaaaagta cgggttgcgg cagatcgggg agcgcgggag
      541 tgttcgggta ccactgcgtt tccagggcaa gcgcaagtcc aggtgcgccg cccctgtctc
      601 cggagaagcg ttgcgacgag ttcagggaac ccccggtgta gaggacgcag gcgggctggt
      661 cggtgaagac ctccatgatg cggccgcttg cagggtattc gaggacgata tccggcgtcc
      721 ttccgttttc cggtgaattg aggaggaacg ggtggtcgta tccgttgccg agctgaatct
      781 ggcggtccga cgcccactgc gaatgttccg gggagcgggg agccctgaag tcgaagggag
      841 tcccgtcgac cgaacggggc atacagggaa gatgctgctc gtcgaggggc atgaaacggt
      901 cggcgtcgat cgccagtcga tggtcggcga ttgacgccgt attttctccg ccgaggttga
      961 agtagccgtg aaacgcaggc gcaagcagcg tcggaacggc agtttcggcc tcccaggcga
     1021 gtatcagcga atttccggta agcgtatagg tcgccgtaac atcgagcgtt cccggaaaac
     1081 cccactggcc cggcagcgtc gagaagcgga atcggacgcc ggcctccccg ggagaagaga
     1141 acgtctctcc gtgccagacg gaataggaaa acccctccgg gccgctgtgc agacatgacg
     1201 tcccgtcgtt ggcttcaagt tcgcacggtc ttccctcgag tgaaaacgaa gcccctgcaa
     1261 tacgccctgc aaccgggccg caaagcagtc cgcaccagca cgtgtcttcc tcgtacccgg
     1321 cgaggcggtc gtaggaaagt acaacattct cgcagacgcc gtcgcgatcc ggaacctcca
     1381 gcgaagtgac ggcagccccg aagtcgatgc agcgtaacgc caaggagccg ttcctcgcag
     1441 tgtgcagaaa aaccttctcc cccgtccgcg tctcgccgaa aagaaccgaa tcggcggaag
     1501 gggcggagga acagtgttcg ctcatgaaga gtcctccttc acaatcggga agcggcccgg
     1561 ttcgcccaaa aaaggggagc gccgggccgc tgtactgcga gctacttcgc ggttttcttc
     1621 ttcgtgtaga cgtcgaaggc gaccgcggcg aggaggacga gtcccttgac cacctgctgc
     1681 cagtcgatgg agacgcctac gatggacatg ccgttgttga gcacgcccat gatcagtccg
     1741 ccgatgatgg cccctgtgac ggtgccgatg cctccggagg cggatgcgcc gccgatgaag
     1801 cacgcgccga tggcgtcaag ctcgaaattg accccggcga cgggagaggc cgcgttgagc
     1861 cgtcccatga aggcaagtcc cgcgaccgcc gccatcaggc ccatgttcgc atagacgagg
     1921 aaaaagactt tgttcgtatc gatacccgag agtttcgccg ccttctcgtt gccgccgaac
     1981 gcgtagacgt gacggccgaa gacggtcttc ctggtgatga agctgtagat aaggacgagc
     2041 gctccaagga gcaccagtac catcggaatt ccgcggtacc gggcgagctg gtaggtaaag
     2101 gccgtgacga ccgcggcgac aagcaggatc ttcatcaccc agaaggggaa cggggcgacc
     2161 tcgaacccgt acgctttttt gttttgacga tcccgcagct cgaagaaggc gaacgcgcac
     2221 aggaggattg cgacgattgt cagggcagtg tagttcagcc cctcgccccc cagcacgtcg
     2281 gggatgaacg tggtgaagag tttacggaag aggtcgggca tgggcgcgag cgtctgccct
     2341 ttcagcatga ccagtgtaag tccgcggaaa cagagcattc ctgccagggt gacgatgaac
     2401 gcgggaatgc ggaaataggc gatccagtag ccctgccagc agccgatcag gatgcccaga
     2461 acgaggcacg caaggatgga ggggatgacg ggccacttcc agaccgtcat cagggttccg
     2521 ccgacggccc cgacgaaagc ggccaccgag ccgaccgaga ggtcgatgtt tccggtgaga
     2581 atgcagagca gcatgccgat cgccaggatg aggatgtagc cgttctgctg aatgatgttc
     2641 gttaagttca gcggacggag gagcacgccg tccgtgaggt actggaaaag aagcacgatg
     2701 aagaggagcg cgagcatcat cgcgtattgt cggatgttca tcatgaacat ctctttcagc
     2761 ttgcgcatca ggccacctcc cgattgctct gcataatgca cttcatgatc gcttcctgcg
     2821 aggcgctctc acgatccaat tcgccgacga agcgcccctc gttcattacg tatatccggt
     2881 cgctcatgcc gagcacctcc ggcatctccg aagagatcag gacgatcgcc ttgcccattg
     2941 ccgccagttc gttgatgatg cagtatattt cgtacttcgc gcccacgtcg atgccgcgcg
     3001 tcggttcgtc gagcagcagc acgtcggggt ttgcgaagag ccagcgggcc agaatggttt
     3061 tctgctggtt tccgccggag agattgccta ctttctgaaa gacgctcggg gtcttgatgg
     3121 acatcttctt ccgcaggtct tcagccaccg tgatctcttt gttctcgtcg agaacgcccc
     3181 gcttgtcgct caacttgccg aggctggcga gggagatgtt gtggcggatg tcgtcgatga
     3241 gaatcagtcc tgcggatttc cggtcctcgg tgacgtacgc catgccgcgg gcgatcgcct
     3301 ccttggggga gtgaagcgtt gccgattgtc cgcccagaaa gagacagccg gaaaaatcgc
     3361 tcccgtagga gcgtccgaag aggctcatgg cgagttctgt ccgcccggcg cccatcaggc
     3421 cgacgagtcc tacgacctcg cccttatgga cgttgaagct gactcctttg atctttttgc
     3481 ggtcgaagaa gagagggtcg ccgacagtcc agtcacggac ttcaagagcg aggtcgcctc
     3541 tcgcgtgagg agggcgtttc gggaatcggt cgacgatctc ccgccctacc atccccttga
     3601 tgatgcggtc ctcccggatg ctttttgtcg cgttatccag cgtctcgatg acggcgccgt
     3661 ctcggatcac cgtgatgcgg tctgcgatct tctccacttc gttgagcttg tgcgagatga
     3721 tgatggacgt aatgcccttc ttcttcaaat ccagaagcag gttcagcaga ttgttgctgt
     3781 cctcgtcatt gagggctgcg gtcggttcgt cgagaatgag caggcgcacg tcttttgaga
     3841 tggcttttgc aatctccacg agctgctgct tccccacgcc gagatctttt gtgatagtcg
     3901 ccgggttttc gtgcagtcgt atctgtttga gaagatcgtc ggtgcgcgcg taggtctcgc
     3961 tccagtcgat cacgccccgc cgggcccgtt cattgccgag aaagacgttc tcggcaatcg
     4021 agaggtaggg gacgagcgcg agctcctggt ggataatgac gatgccgatc ttttcgctgt
     4081 cttttatcgt attgaagacg caggtctccc cgtcgaagac gatctcccct tcgtaggacc
     4141 cgaaggggta gacgccggag agaatgttca tcagggtgga ttttcccgcg ccgttctcgc
     4201 cgacgagcgc gtgaatctcc ccctcttcga cgtcgaaaca gacgtcgttc agagcgcgga
     4261 ctccgggaaa ggtcttcgtg atgctcttca tccgcaggag tgttttggcc atgctctcat
     4321 ctcctgttcc tgattcaagg gggacggagc gcgatggccc cgtcccctcc gtcacgatat
     4381 gctgcgtgag tgttcccgga ttacttcaga tcctcttcct tgtagtagcc ggaatcgagg
     4441 agcagttcct tgtagttgtt gacgtcggcg aagacgggct caagcaggta ggaaggaacg
     4501 accttcatgc cgttgtcgta ggtcttcgtg tcgttcactt cgggctgttt gccctggata
     4561 atatcgtcga ccatctcggc gacctttgtg gcgagcgcgc gggtgtcctt gaagacggac
     4621 atcgactgct tcccggcgat catgttcttc acgttcgcgc ggtcgcagtc ctggccggtg
     4681 atgatgggcc agtctttgcc ggggatgtag ccgttgttct caagggaggc ggagatgccg
     4741 taggcgagcg agtcgttggg ggagagaacg gcgtcgagct tcttgtcggt gtacgacctg
     4801 gagatgaggt tgtccatacg agcctgagcg tcctcggatt tccatgcgag aatgccgatc
     4861 tgctccatct tcgtctggcc ggagtggcag acaagcttgc cgctgtcgag gtagggctgc
     4921 aggatgctca ttgcgccgcc gttgaagaag aacgcgttgt tgtcgtcggg ggaaccgccg
     4981 aagaactcga tgttgaaggg acccttgccg tctttcagtc caagcttctc ttcgatgaac
     5041 ttgccctgga tgacgccgac cttgaagttg tcgaacgtgg cgtagtaatc cacgttgggg
     5101 gactggcgga taaggcggtc gtaggcgatg acggggatct tcgcttccgc ggccttggcg
     5161 aggacgtcgg agagagccga accgtcgatg gaggcgatga tcatgacctt gacgcctttg
     5221 gtaatcatgt tctcgagctg ggagatctga acgctgacgt tgttctcggc gtactgcaga
     5281 tcgactttat agccttttcc ctcgagaatt ttcttcatgt tcgcgccgtc ttcattccag
     5341 cgctggagcg actgcgtggg catggcgacg cccacaagaa cgtccgctgc gaaagcggct
     5401 gtggcggaga agagaaggac aagcgcgaaa agagcgagca gcattttctt cataaaaacc
     5461 acactccttt gaagatggta acgaagggct tctattacgc ccctccgcgt cctccttccg
     5521 ggaggacttc cggcgcctcg gaggcgaact gtgaagcgga cccccgcagg gcgggggaga
     5581 gaaccagttc aggaagtgct cgagagggca tcatctcctt atctaagcag aatacggctt
     5641 gcggttcgtc ttgtcaaggc taattgattg attcaataaa tatattatga acatttacga
     5701 ttctcttgtc aagagggggg caaaaaaact ttttcggcgc cggcgcctcc gggagttcgg
     5761 cctttaaaac gaagtcctcg ttttgcggaa agggcatgac tttggagaaa ccaggcagcc
     5821 ggacttggaa tatcggtgat tctgcggaga agaagaggaa agcggctgcg ggtaaagaat
     5881 atggtacaat cgtccaaaac aggacgtcgt acgggaacac gacgctgtct cttcgcactt
     5941 cttgcgaaga cgcttcgcgt tcctgcccaa agaatctcta cgcgggtttt cgcccgtatt
     6001 ctcaacgtct ccctcgcctg ttttcaaggc ggggtttgtt atgtgcaggc agaaggagga
     6061 agtccattgt tgaccggaaa gagagttatc gtgtgctgtg cgcttgctgc ggggctgctc
     6121 cttcgcctcc cgttcgcggg tggagcggag gaaaccgcag gaccaacggc aatggaagag
     6181 ggggaagctg tctttcagtt gtcttcgggc gacgtgtccg cagcgtccgt tctttcacag
     6241 tctccggctg tcgaagaggc ggcgggtccg ctccagacag ctgagaagac ctccgaagag
     6301 ggaattccgg atgctccgtc cgcagagact gccgggaacg agctgggagc aacctccgca
     6361 gacgtccccg tgtccgcagg gtcggagcag cttgccagga agctcttcga gattgaggtt
     6421 gccagccgcg tcgctgaaga agagcttgga aagctcccga gcctcgtaca gtccgaacgg
     6481 acgaagattg cgaacgaact gaaccagtgg gcaaacacgg atcttcgcag aatcatcgat
     6541 cagcactacc tggaggtgga gaaggcgctc gcggagacga cgcttcccga ggaccatagt
     6601 tccgtgggca tctggtggaa gcaggtgaag gcgggcgttt tcctgaacga ggaaatcgag
     6661 gaggtcgtcg ccctcttcgt gaaacgcgca ggcccgaggc tctccaccgt gcacgaggag
     6721 tttcagcgaa cgctgctcga aagtttggag cagcgcagcc agacgcttct gcgccgttct
     6781 atggaagaga ttcgcacacc attcgtcgct acggtcaaag acagacttcc tctttacaat
     6841 gcaatcccgg tgcccaatac ggagcgcgcc gcattgtcga tcgccctttc aggggaacag
     6901 agcgacaggg gacgtctctc gtcaggtatg gctgtgatcg gcggcgttgt gctcaccgtt
     6961 cttgggcgga agctggtcca gaaaatgatg cacctggcgg tggtcaagat cggagggaaa
     7021 gtgctctcgc ggatgatccc catcatcggg gtcggcttgc ttctgtacga gggcatccag
     7081 atgggacag
//
LOCUS       DAOTIL010000027         7024 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminobacteriaceae bacterium isolate AMR_MDS_3026
            NODE_12773_length_7024_cov_3.580284, whole genome shotgun sequence.
ACCESSION   DAOTIL010000027 DAOTIL010000000
VERSION     DAOTIL010000027.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868050
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminobacteriaceae bacterium (wastewater metagenome)
  ORGANISM  Aminobacteriaceae bacterium
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae.
REFERENCE   1  (bases 1 to 7024)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7024)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:19:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,224
            CDSs (total)                      :: 3,198
            Genes (coding)                    :: 3,184
            CDSs (with protein)               :: 3,184
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7024
                     /organism="Aminobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12773_length_7024_cov_3.580284"
                     /isolate="AMR_MDS_3026"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:3034609"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..672
                     /locus_tag="P5201_01070"
     CDS             <1..672
                     /locus_tag="P5201_01070"
                     /inference="COORDINATES: protein motif:HMM:NF014533.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009294 - DNA-mediated transformation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-processing protein DprA"
                     /protein_id="HRV97153.1"
                     /translation="SGGAAGIDKAAHEGCLSAGGASAAVLGTGVDVAYPRGHEPLFVR
                     LAEEGGALLSEYPLGSQPKQWRFPERNRIIASLADRLVVVEAPSKSGAMITARCALEA
                     GREVWAVPGRINESGCEGSNRLLYDGAVPLVSIPDFISLALGRQLSLFEETPFRGEQN
                     SVAFTEKEQRILCLLEKSGERTVDNLAVEGTMAPADVFSCLAALMAAGRVFVAGPGRW
                     SAVPK"
     gene            704..1030
                     /locus_tag="P5201_01075"
     CDS             704..1030
                     /locus_tag="P5201_01075"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRV97154.1"
                     /translation="MPIIKKICGTRGRITESLEFKRVLHDLERRYLSPDAQAKIYRYL
                     ATEEVPLEVTERAILEAVALARLKNATADASLFDFLVDALLDDCSFEIPETASERVYP
                     SSCTLC"
     gene            1046..3487
                     /gene="topA"
                     /locus_tag="P5201_01080"
     CDS             1046..3487
                     /gene="topA"
                     /locus_tag="P5201_01080"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013048901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003917 - DNA topoisomerase type I (single
                     strand cut, ATP-independent) activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I DNA topoisomerase"
                     /protein_id="HRV97155.1"
                     /translation="MVRKKIVTEAEVVESPSAPPKKGGKTAAKAEQGTAAPVKKAAPK
                     SASKKNLSSPSAKPKKTASSKTSAAKASSEKTLSADALPKKPVKKQAAKKSPASLPKT
                     KTALSSAAGSSDRTLVIVESPTKAKTLTKILGSKYTVKSSVGHIRDLPKSRLAIDIEN
                     DFAPEYILVKGKAKLKNELLAAAKQSPRVLLASDPDREGEAIAWHLAELLDIPLDTEC
                     RVRFYEITPTAVKEALAHPERVNMHKVEAQQARRILDRLVGYTLSPLLWKKIRRGLSA
                     GRVQSVALALICEREREIEAFVPKEYWIVTVLAVAEDGRVYSLKVDSFDGKSLWKEGK
                     SLLIDSEEFVDVLVGEILKAPLTVSDFKVRESYRAAPAPFKTSTLQQESARRNSMSPR
                     RTMSIAQELFEGVSLPDRGPTGLITYMRTDSLRIAPEALETCRQYIASAFDPEYLPEK
                     ANEYASKGRSQDAHEAIRPTDVTIVPKSLEGILTNDQMKLYSLIWNRYVASQMSKAKV
                     ANATLAATAGKVGLRQLGETLVFPGWSAVWPLDLKGESFAPARAGETLRVEKTEREQK
                     FTRPPARYSEASLIKVLEDEGVGRPSTYASIVETLYDRGYVARNEERRLQPSELGKTV
                     DSFLRKYFDAESLSAIVDTGFTARMEESLDDVEESNISWLAVLKEFWDAFTRTMQDAE
                     KAPAEKLPPSEPTGEDCPECGKPLVLKNGRFGDFIACSGYPDCKFTKPVLVKTGAVCP
                     KCGEGDVVKRKSRKGKPFYGCSRYPACDFVSWNPPSGETCPQCGAALFAKGTKGAEEC
                     MKCGFTRVRETAADE"
     gene            3480..4838
                     /gene="trmFO"
                     /locus_tag="P5201_01085"
     CDS             3480..4838
                     /gene="trmFO"
                     /locus_tag="P5201_01085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009164352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylenetetrahydrofolate--tRNA-(uracil(54)-
                     C(5))-methyltransferase (FADH(2)-oxidizing) TrmFO"
                     /protein_id="HRV97156.1"
                     /translation="MNRRVTVVGGGLAGSEAAWQLARRGIEVLLLEMRPLRTTPAHTT
                     EMLAEVVCSNSFGADSAASPAGILKNELRRLDSLILSCADESRVPAGKALAVDREIFS
                     RKVTERLESLPQVAVRREECVSLPEGPVIVATGPLTSPALAAILQRQAGTEYLSFFDA
                     VAPVVVADSVDMSVAFRRGRWGQEEDYINCPFTRERYEAFWNALVAAERALPHDFEDS
                     PSWFEGCLPVEVMASRGIDTLRFGPLRPVGLPLPGTGEEAWAVVQLRQDNREGTLFNL
                     VGFQTSLRWGEQERVFRMIPGLENAEFARLGVMHRNIYVNAPATLDKHLRFREGRTDL
                     FLAGQITGVEGYMESTAMGCVAALNAAALVDGVPFPEWPRATSIGSLLHYLGDAEPKH
                     FQPMNMNLGIMPKPEGKIRNKALRCEYVAEVASKAFDSFLESLKADSQTDDCQSEISG
                     ER"
     gene            5002..5901
                     /locus_tag="P5201_01090"
     CDS             5002..5901
                     /locus_tag="P5201_01090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008712029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0009037 - tyrosine-based site-specific
                     recombinase activity [Evidence IEA];
                     GO_process: GO:0006313 - transposition, DNA-mediated
                     [Evidence IEA];
                     GO_process: GO:0007049 - cell cycle [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine recombinase XerC"
                     /protein_id="HRV97157.1"
                     /translation="MLENVWSTIDLFLDHLRFEKEASPHTVTNYAADLAQFAEFLDHQ
                     GVVRPESVDSRIVRFFLREIMGFGYSKTSVSRKLSAVRSWSAFLKMRGVLASDPAKGV
                     RGPKLPKLLPRALSREDAQLLVEDGPDGGDRVRDRAILELLYGCGLRIAELVSLRWEA
                     MEPVEERMLRVAGKGEKERLVPFGQCALAALREWRPFVPPDAPFLFPGLKGGAITVRT
                     VHRVVLRAAARVGLSGVTPHTLRHSFATHLLEGGATLAVVKELLGHESLLTTQRYLAI
                     NAEHLRESYRGAHPRAKGEDGLV"
     gene            5894..6427
                     /gene="hslV"
                     /locus_tag="P5201_01095"
     CDS             5894..6427
                     /gene="hslV"
                     /locus_tag="P5201_01095"
                     /EC_number="3.4.25.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009201698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004298 - threonine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0051603 - proteolysis involved in protein
                     catabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent protease subunit HslV"
                     /protein_id="HRV97158.1"
                     /translation="MFKGTTVICVRRGEQVAMAGDGQVTLGNQIIKAGARKVRRLYKG
                     SVLAGFAGSTADAMTLLERFESRLEENSGDLMRSAVAMVKEWRTDRALRRLEAMMIVA
                     DAKLSLLLSGAGDILEPENNIASIGSGSGFAQAAGMALLETSDWAPSKIAKRSIEIAS
                     EICIYTDSIVTVEAIGE"
     gene            6424..>7024
                     /locus_tag="P5201_01100"
     CDS             6424..>7024
                     /locus_tag="P5201_01100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009201697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HRV97159.1"
                     /translation="MNSAIEEMELTPRRIVEYLDRYIVGQEKAKKSVAIALRNRIRRR
                     NLSPEMAKEVAPKNILMVGPTGVGKTEIARRLAELVKAPFVKVEATKFTEVGYVGRDV
                     ESIIRDLAEAAVAMVKKRKIEEVQTPAAERAEQRLADALLPGSEKKPSMPDFMKIFGG
                     GREREEAPQPAPEEERMRESTRRKLCTLLEEGKLDDREVE"
BASE COUNT         1417 a         2069 c         2228 g         1310 t
ORIGIN      
        1 agcggcggag cggcggggat cgacaaggct gcccacgaag gatgcctttc cgctggcgga
       61 gcaagcgccg ccgtcctcgg aacaggcgtc gacgtcgcct accctcgcgg ccacgaaccg
      121 ctctttgttc gcctcgccga agagggaggc gcgctgctct cggaataccc cctcggttcg
      181 cagcccaagc agtggcgctt tccggagcgc aaccgcatca tcgcgtcgct cgccgaccgt
      241 ctggtcgtcg tcgaggcgcc ttcgaagagc ggcgccatga ttaccgcccg ctgcgccctc
      301 gaagcgggac gggaagtatg ggcggtcccg ggaaggatca acgaatcggg gtgcgaaggt
      361 tccaaccgtc ttctgtacga cggcgccgta ccgctggtgt cgatacccga cttcatttcc
      421 ctcgcgctcg gacgccagct ctccctcttc gaagagacgc cgttccgggg agagcaaaat
      481 tccgttgcct ttactgaaaa agagcaaaga attctttgcc ttttggaaaa atcaggggag
      541 agaactgttg acaaccttgc tgttgagggt acaatggctc ccgctgatgt attttcctgt
      601 ctcgccgcgc ttatggctgc gggacgtgtt tttgtagcgg gaccgggccg ttggagcgcc
      661 gtcccgaagt aactccaaac cattcgggaa aggaagaaac ctggtgccca ttataaaaaa
      721 aatttgcgga acccgggggc gcatcactga gtctctcgag ttcaaaagag tgctccatga
      781 tctggaacgg aggtatctct cccctgacgc acaggcgaag atttaccgct atcttgctac
      841 agaggaagtg ccgctggagg tcacggagcg ggcgattctt gaagcggtcg ctcttgcccg
      901 tctcaagaac gccactgcgg acgcttctct ttttgatttc ctcgtagacg cgctgctcga
      961 cgactgttcc ttcgagatcc ccgagacggc ctcggagagg gtctatcctt cgagctgcac
     1021 cctttgctga ggacggtttt ttcgcatggt tcgcaaaaag atcgtaactg aagcggaggt
     1081 tgtagagagt ccctccgctc cgccgaagaa gggcgggaag acggccgcga aggctgaaca
     1141 agggactgcg gcgcccgtga agaaggccgc cccgaaaagc gcttcaaaaa agaatctttc
     1201 gtctccttcg gcgaaaccga agaagacggc ctcgtcgaag acctccgctg cgaaagcgtc
     1261 ttccgagaag acgctttcag cggacgcgct cccgaagaaa cccgtgaaaa aacaagcggc
     1321 gaaaaagagt ccggcttccc tgccgaagac gaagacagcg ctttcttccg cggcaggctc
     1381 ttcggacagg acgctcgtca tcgtggagtc tcccacgaag gcgaagacgc tcacgaagat
     1441 ccttggctcg aagtacacgg tgaagtcgag cgtcggccac attcgcgatc tgccgaagag
     1501 cagactggcc atcgatatcg agaatgactt cgcccccgag tacatcctcg tcaaggggaa
     1561 ggcgaagctg aagaacgagc tgctcgccgc ggcgaagcag tctccgcgcg ttctgctcgc
     1621 ctccgacccc gaccgcgaag gcgaggcgat cgcgtggcat cttgcggaac tgctcgacat
     1681 cccgctcgac acggaatgcc gcgtccgttt ctatgaaatc acacccacgg cggtgaaaga
     1741 ggcgcttgcg caccccgagc gcgtcaatat gcacaaggtg gaggcgcagc aggcgcggcg
     1801 cattctcgac cggctggtgg ggtatacgct gagtccgctt ctctggaaga agatacgcag
     1861 ggggctctcc gcagggaggg tccagtctgt tgcgctcgcc cttatctgcg agcgggagcg
     1921 cgagatagaa gcgtttgttc caaaggagta ctggattgtc acagtgctcg ctgttgccga
     1981 ggacggaagg gtctattcgc tcaaggtgga cagcttcgac gggaagagcc tctggaagga
     2041 agggaagtcc ctgctcatcg actcggagga gtttgtggac gtcctcgtcg gcgagatcct
     2101 gaaagctccc ctcacagttt ccgacttcaa ggtgcgcgaa agctaccgcg ccgccccggc
     2161 gccattcaaa acaagcacgc tccaacagga gtccgcccgc aggaacagca tgtcgccccg
     2221 gcggacaatg agcatcgccc aagagctttt cgaaggcgtc tctctgcccg acagggggcc
     2281 gaccggcctc atcacctaca tgcgcaccga cagcctcagg atcgctcccg aggcgcttga
     2341 gacgtgcaga cagtacatcg cctccgcctt cgatccggag taccttccgg aaaaagcgaa
     2401 cgaatacgcg tccaaaggac gctcccagga cgcgcacgag gcgatccggc cgaccgatgt
     2461 gaccattgtg ccgaagagtc tcgaaggtat cctcacaaac gaccagatga agctctactc
     2521 gctcatctgg aaccgctacg tggcctcgca gatgtcgaag gcaaaggttg ccaacgcgac
     2581 gctcgccgcg accgccggca aggtcgggtt gcgccagctc ggcgagacgc tcgtctttcc
     2641 ggggtggagc gccgtctggc cgctcgacct caagggggag agcttcgctc cggcccgcgc
     2701 cggggagacg cttcgcgtcg agaagacgga gcgcgaacag aaattcacgc gcccgcccgc
     2761 acgctactcc gaggccagcc tgatcaaggt cctcgaggac gaaggcgtcg gccgtccctc
     2821 gacgtacgca tccatcgtgg agacgctcta cgacagaggg tacgtcgcgc gcaacgaaga
     2881 gcgccgcctt cagccctccg agctcgggaa aacggtggac tccttcctta ggaaatactt
     2941 cgacgcggag agcctctccg cgatcgtcga cacaggcttt accgcccgca tggaggagag
     3001 tctggacgac gtcgaggaga gcaatatctc ctggctcgcc gtcctcaaag agttctggga
     3061 cgcctttacc cgcacgatgc aggacgcgga gaaggccccg gccgaaaagc ttccgccctc
     3121 ggaacctacc ggcgaagact gtcccgagtg cggcaagccg ctcgtgctca agaacggccg
     3181 tttcggcgat ttcatcgcat gtagcggcta ccccgactgt aaattcacca aaccggtcct
     3241 tgtgaagacc ggggcggtct gtccgaagtg cggcgaggga gatgtcgtta agcgtaagag
     3301 ccgcaagggg aagcccttct acggctgttc gcgctacccg gcgtgcgact tcgtctcctg
     3361 gaatcccccc tcgggggaga cgtgtccgca gtgcggcgcg gctcttttcg ccaagggaac
     3421 gaagggcgcg gaggagtgca tgaagtgcgg ttttacccgg gtacgggaga cggctgcgga
     3481 tgaataggcg cgtcaccgtc gtcggaggcg gactcgccgg ttcggaggcc gcgtggcagc
     3541 ttgcgcggcg cggtattgag gtattgctgc tcgaaatgcg cccccttcgg acgactccgg
     3601 cgcacaccac ggagatgctc gccgaagtcg tctgcagcaa ctccttcggc gcggacagcg
     3661 ccgcgagtcc cgccggaatc ctcaagaacg agctgcgccg tctggacagc cttatcctct
     3721 cctgcgccga cgaatctcgc gttcctgcgg gcaaggcgct cgccgtcgac agggaaatct
     3781 tctcgcgaaa ggtcacggaa cggctcgaat cgcttccgca ggtcgccgtt cgcagggagg
     3841 agtgcgtctc cttgcccgaa ggaccggtga tcgtcgccac ggggccgctg acatccccgg
     3901 cgcttgccgc gatcttgcag cgacaggctg gaacggagta cctctccttc ttcgacgccg
     3961 tcgcccccgt cgtcgtcgcc gactctgtcg acatgtccgt cgccttccgc cggggacgct
     4021 gggggcagga ggaggactat atcaactgcc cgttcactcg cgaacggtac gaggcgttct
     4081 ggaacgccct cgtcgccgcg gagcgggcgc ttccgcacga cttcgaggat tctccctcct
     4141 ggttcgaggg gtgtctcccc gtcgaggtga tggcttcgcg cggtatcgat accctccgct
     4201 tcggtccgct gcggccggtg gggcttcctc tgcccggcac gggggaagag gcatgggctg
     4261 tcgtgcagct tcgtcaggac aaccgtgaag ggacgctctt taatctcgtc ggctttcaga
     4321 cgagcctccg gtggggggag caggaacgcg tcttccggat gatccccggg cttgaaaacg
     4381 ccgaattcgc ccgtctgggc gttatgcaca ggaacatcta cgtcaatgcc ccggcgacgc
     4441 tcgacaagca tctgcgcttc cgcgaaggca gaacggatct cttcctcgcc gggcaaatca
     4501 ccggcgtcga ggggtacatg gagagcaccg cgatggggtg cgttgcggcg ctcaacgcgg
     4561 cggcgcttgt tgacggcgtt ccgtttccgg agtggccgag ggcgacctca atcggttcgc
     4621 tgctgcacta cctgggtgat gcggagccga agcacttcca gccgatgaac atgaacctcg
     4681 gcatcatgcc gaaaccggag ggaaagattc ggaacaaggc gctccggtgc gaatacgtcg
     4741 ccgaggtcgc atccaaggct ttcgattctt ttcttgaatc attgaaggcg gattctcaga
     4801 cggacgactg ccagtctgaa atttccggtg agcggtaaac gacttgtttt ttctcataat
     4861 tctctttgta taaaaatatg ctcttcagat cattttctgt gctcaatgca ctgttcatgc
     4921 ggttttgcac aaaagaaacc atgccttaat tgattctttt gaaaaatagc gagaatctag
     4981 aaaacgtgat aaaatgtctc tatgttggaa aacgtctggt caaccataga tctttttctt
     5041 gatcatctgc gatttgaaaa agaagcctcg ccgcatacgg tgaccaacta cgctgcggat
     5101 ctggctcagt ttgcggagtt ccttgaccac cagggagttg tgcgcccgga gagcgttgat
     5161 tcccggatag tacgcttttt tcttcgggag attatggggt tcggctactc aaaaacatcc
     5221 gtctccagga agctctccgc tgtgcgaagc tggtcggcgt tcttgaaaat gcgcggcgtg
     5281 ctggcttccg accccgcgaa gggagtacgg gggccgaagc ttccgaagct cctgcccagg
     5341 gcgctttccc gcgaggacgc gcagcttctg gtcgaagacg ggccggatgg cggcgaccgg
     5401 gtccgcgaca gggcgatttt ggagctgctc tacggttgcg gtcttcggat cgcagagctt
     5461 gtctcgctgc gatgggaggc catggagccg gtcgaggaac gaatgcttcg cgttgcaggc
     5521 aagggagaga aggagcgtct tgttccgttc ggccagtgcg cgctcgccgc gcttcgcgaa
     5581 tggcgtccgt tcgttccgcc cgatgcgccg tttctctttc ccggcttgaa gggcggcgcc
     5641 atcaccgtcc ggacggtgca cagggttgtg ctccgcgcgg cggctcgggt gggcctgagc
     5701 ggtgtgacgc cgcacacgct gcggcacagc tttgccacgc atctccttga gggaggcgcg
     5761 acgcttgcgg tcgtaaagga actactgggg cacgagagcc tgctgacaac gcagcgctac
     5821 cttgccatca atgcggaaca tcttcgggag agctatcgtg gcgcccaccc ccgcgcgaaa
     5881 ggagaggacg ggcttgttta aaggaacaac cgtgatttgt gttcgacgag gagagcaggt
     5941 ggcaatggcc ggggacggcc aggtcacatt agggaatcag ataataaagg cgggcgcgcg
     6001 gaaggtccgc agactctaca aagggagcgt gctcgcagga ttcgccggga gcaccgccga
     6061 cgccatgacg ctcctggagc gttttgaaag ccgcttggag gagaattccg gcgatctgat
     6121 gcgttcggcg gtggcgatgg tcaaggagtg gcgtaccgac agggcgctcc gcaggctgga
     6181 agcgatgatg atcgtcgcgg acgcgaagct ctcgctgctt ctttccggag ccggcgatat
     6241 ccttgagccg gagaacaaca tcgcttcaat cggctccgga tcggggttcg cgcaggccgc
     6301 cggaatggcg cttctcgaaa cgtccgattg ggcgccgtcg aaaatagcga agcgctctat
     6361 tgagatcgct tcggagatct gcatttatac agacagcatt gtgactgtgg aggcaatagg
     6421 agaatgaaca gcgccattga agaaatggag ctcacgccgc gccggatcgt cgagtatctc
     6481 gaccggtaca tcgtgggcca ggagaaagca aagaagtcgg tcgccattgc gctgcgaaac
     6541 cgtatccgga ggagaaatct ctctcccgag atggcgaaag aagtggcgcc gaagaatatc
     6601 ctcatggtgg ggcctaccgg cgtcggcaag accgagatag cccgcaggct tgcggagctg
     6661 gtgaaagcgc cgttcgtcaa ggtggaggcg acaaagttta ccgaagtggg gtatgtgggc
     6721 cgcgacgtcg agtccatcat cagagatctc gccgaggccg cagtggccat ggtcaagaaa
     6781 cgcaagatag aagaggtgca gaccccggcg gcggagcggg cggagcagcg tctcgccgac
     6841 gcgctgcttc cgggatcgga gaagaaaccc tccatgcccg actttatgaa gatcttcggc
     6901 ggcggacggg agcgcgaaga ggcgccccag cctgctcccg aggaggagcg catgcgggaa
     6961 tccacccgca gaaagctgtg cacgctgctc gaagagggga agctcgacga ccgcgaggtc
     7021 gaga
//
LOCUS       DAOTIL010000028         6944 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminobacteriaceae bacterium isolate AMR_MDS_3026
            NODE_12957_length_6944_cov_3.690086, whole genome shotgun sequence.
ACCESSION   DAOTIL010000028 DAOTIL010000000
VERSION     DAOTIL010000028.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868050
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminobacteriaceae bacterium (wastewater metagenome)
  ORGANISM  Aminobacteriaceae bacterium
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae.
REFERENCE   1  (bases 1 to 6944)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6944)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:19:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,224
            CDSs (total)                      :: 3,198
            Genes (coding)                    :: 3,184
            CDSs (with protein)               :: 3,184
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6944
                     /organism="Aminobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12957_length_6944_cov_3.690086"
                     /isolate="AMR_MDS_3026"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:3034609"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..218
                     /locus_tag="P5201_01105"
     CDS             <1..218
                     /locus_tag="P5201_01105"
                     /inference="COORDINATES: protein motif:HMM:NF012451.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DnaJ domain-containing protein"
                     /protein_id="HRV97160.1"
                     /translation="PGGKRDPYTVLGCSPSSSNEEIKKRYRELLAKYHPDKFIGQNLD
                     EDFVALASRKFQEIQEAYGRIRSARGI"
     gene            413..2074
                     /locus_tag="P5201_01110"
     CDS             413..2074
                     /locus_tag="P5201_01110"
                     /EC_number="4.1.1.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013048943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004612 - phosphoenolpyruvate
                     carboxykinase (ATP) activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxykinase (ATP)"
                     /protein_id="HRV97161.1"
                     /translation="MSTLELFTGPESFSRIKSQARTTIESAFFGNNVVPVSNLKEAYE
                     LARVSPGTVELTGMPVYRPEGQGLPPRSNVLLMNDGAVVGRCAAARRIVGEPGVNMGD
                     YAAVLREAVYNSRFRTFYSAEAYIGLEGDFMVKAHLLAPKNHENLVYNWLLNFQYLNR
                     TYLDMYAASRPLPDGDIFMFADPDWTSPDYPLGLAFFDPLHNCAAVLGMRYFGELKKG
                     TLTLAWGTAVRNGFASCHGGLKRYNLKGGRSFVAAVFGLSGSGKSTITHARHGNKYDI
                     TVLHDDAFVINVKEKYAIALEPSYFDKVQDYPMGCEDNKFIITLQNCGVTRGEDGLLY
                     AVTEDLRNGNGRAIKSKLWSPERVDRIDEPIDAVFWLMKDSSLPPVLRLKGSDLAAVL
                     GATLATKRTTAERLAPGVDPNALVIESYANPFRTYPLATDYERFKALFDDGVACYILN
                     TGEFMGKKVKPADTLGILEALVEGKAEFVPFGPFSDMETMALDRFPIDFTDEQYRREL
                     FSRMRDRLAFVTSRETEKGGMDRLPPEAVAALRKALDEMGYPVQE"
     gene            complement(2103..2660)
                     /locus_tag="P5201_01115"
     CDS             complement(2103..2660)
                     /locus_tag="P5201_01115"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRV97162.1"
                     /translation="MTEAFLFPLRLHLLCAPSHRRGEYLLERKFAQAFAASNGIPLDF
                     DALMATLREWCASQGVVRNGQTASFSGKSADKKYSGTATRFRDELSILIHVDGEGRKR
                     FRILGLWNDFSWLVLYQEPLLGEWRSWPGAAKDPEGMEKDRTDERSAREGFEWVCRRR
                     IISRARLLRGEEVTTEYYSPSYRKR"
     gene            2755..4158
                     /locus_tag="P5201_01120"
     CDS             2755..4158
                     /locus_tag="P5201_01120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013048942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042910 - xenobiotic transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006855 - xenobiotic transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HRV97163.1"
                     /translation="MEKNLNNSHAMGTDSITRLMWRFSLPAIVGMLANALYNIVDRLF
                     VGQTVGAAGIGAISVVFPFMILVISFDLLVGVGAGSLISISLGEKRRARAERAMGNAI
                     MLLVGGGILLVLLGVSFTESILELSGASATLMPLCLEYMNIAVWGVFFSTLAFGLNFF
                     IRAEGNPRYAMFTLIIGAGANVLLDALFILVFDMGVGGAALATLFSQMISALWAALYY
                     IRRMGTLRFRMKNLAPDWVITRRILAIGSAPALTEVSFTYVLALFNRLLSFYGGDLAI
                     SAMGIFFSLDSLFFLPVLGLAGGVQPIISYNYGAKDYDRVIRSVLAAIKVGALFFTVS
                     FTLVMLFPAPLIRIFNSDDPELLALGVRGMRLAYSGVIFASVSLIASHTFQAIGKAKT
                     GLFLTLSRHFIFILAPLYFLPPILGMDGIWLSLPLSDLGGALTGTWFLRREFRIMRNA
                     ERLQQISAPGECSVSPD"
     gene            4209..5204
                     /locus_tag="P5201_01125"
     CDS             4209..5204
                     /locus_tag="P5201_01125"
                     /inference="COORDINATES: protein motif:HMM:NF012955.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HRV97164.1"
                     /translation="MRASVHPFPEGVCEVSTEEGAALFLIDLPIALPGFDHHLSSWLL
                     RDFKRGRNILIDTGPSASVPFLERSLRSLGVERLDLVLLTHIHADHAGGAGRLLERFP
                     EARVLAPERGRTHLVHPSRLAEGTRATLGAAMADAFGPILPVQKERLIDSSDPLLEGF
                     TVLETPGHASHHSSYVFHADFGKILFPGEAAGVGLGEAVRSLWTAPGMPNVSAPYRFP
                     ATPPRLDGEALERSMELLSAESGAKYLCFSHYGWTTEVAETLNLAAVQFRDWCERARR
                     CAAADEEDAVALLFDETQREDPLLGLLSSLRTEQRQKEEFFIKNSLRGIVRWVRS"
     gene            5281..6561
                     /locus_tag="P5201_01130"
     CDS             5281..6561
                     /locus_tag="P5201_01130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013047734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1576 domain-containing protein"
                     /protein_id="HRV97165.1"
                     /translation="MTEQSGKLGFDRYLVGEGGKRLFLFLFGGLLCTVGLHSSPFAEI
                     LPGLKLIIFEPDYLVSDYFHVGGRGASFLNSGILTLLFTAILVYYKIHIRGISIAAVF
                     TVAGFAFFGKNLLNVWFILGGVWLYAWYRKESFLKFIYIAFFGTALAPLVSQLMFGLY
                     LPAPFRIAAGAIAGLSAGFILPSLAASFLSVHKGYNLYNVGFTAGMVGTLYVSLFRSH
                     GFVPEARVLWSTGNNGLLLPMLLGMFLTMILAGYLLNGRSFKGVSSIWAHPGRLLADF
                     VDIFDFPPTLINMGVNGLLVTGYLLAVGGDFNGPTLGGIFTVVGFSAMGKTPRNIAPI
                     IAGVVLGSLTKHWSLSDPAIQLAALFGTTLAPIAGEFGWKAGILAGYVHSSVVLYVGV
                     LHAGFNLYNNGFAGGLVAAILVPLIETFRGREKE"
     gene            6558..6908
                     /locus_tag="P5201_01135"
     CDS             6558..6908
                     /locus_tag="P5201_01135"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRV97166.1"
                     /translation="MKYERHVVSRIVSELMNFFFSMGARDFSSHVIRNEDRFEICVES
                     DYAGNPTTKIREMTRLLRMPRAREMEEYSWSLSGDISTGQEIYLVGIMTDDVTVEHDE
                     EAGRVRICLTRLLE"
BASE COUNT         1257 a         2077 c         2087 g         1523 t
ORIGIN      
        1 ctcccggcgg gaagcgcgac ccctataccg tgctcggctg ttcgccttcc tcctccaacg
       61 aggagatcaa gaagcgctac cgggagctgc tcgcgaagta ccaccccgac aagttcatcg
      121 ggcagaatct cgacgaggac tttgttgccc tcgcctcaag gaagtttcag gagattcagg
      181 aggcgtacgg ccggattcgc tccgcacggg ggatatagtt tttatctttc ttttcttcgg
      241 agcgcggaac ccgacggcat ctttcttgcg cgactgcggc gggttcttcg gagcgcggca
      301 tgcgtggcga cggtttcaca ccgcggtatg gagtgtgata caatgacgcc gccagagctc
      361 ccctttcgag gggaaaaacg ttcccgcatg cgggagaaag aggcgaacct ccatgtcaac
      421 tctggaactt tttaccggac ccgagagctt ctctcgtatc aagtcacagg cgcggacgac
      481 tattgaatcc gcttttttcg gcaacaacgt ggttcctgtc tcgaacctca aggaggccta
      541 cgagctggcg agagtctccc ccggcacggt cgagcttacc gggatgccgg tctaccgtcc
      601 cgaagggcag ggattgcccc cgcgcagcaa tgtgctcctg atgaacgacg gcgctgtggt
      661 cggccgttgc gccgccgcgc ggaggatcgt cggcgaaccc ggcgtaaata tgggagatta
      721 tgcagcggtg ctccgggaag cggtatacaa ctcccgtttc cgtacctttt actccgccga
      781 ggcgtatatc ggtctcgaag gtgatttcat ggtgaaggcg catcttctcg cgccgaagaa
      841 tcatgaaaat ctcgtctaca actggctgct gaacttccag tatctcaaca ggacctatct
      901 cgacatgtac gcggcatcca ggccgctgcc ggacggcgat atcttcatgt tcgccgatcc
      961 cgactggacc tctcccgact accccttggg gctggcattc ttcgatccgc tccataactg
     1021 cgcggcggtg ctgggtatgc gctacttcgg cgagctcaag aagggaacgc tgacgctcgc
     1081 gtggggaaca gccgtgcgga acggcttcgc ctcatgccac gggggcctga agcggtacaa
     1141 cctgaagggg ggacgctcgt tcgtcgccgc ggtcttcggc ctctccggtt ccgggaagtc
     1201 gacgatcacc catgcgcggc acgggaacaa gtacgatatc accgtccttc acgacgacgc
     1261 cttcgtgatc aacgtcaagg agaagtacgc catcgcgctc gagccctcct acttcgacaa
     1321 ggtgcaggac tacccgatgg ggtgcgagga caacaagttc atcatcacgc tgcagaactg
     1381 cggcgttacc cgcggggagg acggcctgct ctacgcggtg accgaggacc tgcgcaacgg
     1441 caacgggcgc gccattaagt caaagctctg gtcgcccgag cgggtggacc ggatcgacga
     1501 gccgatcgac gccgttttct ggctgatgaa ggactcgtcg ctgccgccgg tgctccgcct
     1561 gaaggggtcc gacctggccg cggtgctcgg cgccaccctc gcaacaaaac ggacgacggc
     1621 cgagcgactc gcccccggag tggacccgaa tgcgctcgtc atcgaatcct acgcaaatcc
     1681 tttccgtacg tatccgcttg ccacggacta tgagcgcttc aaggcgctct tcgacgacgg
     1741 ggtggcgtgc tatatcctga atacaggcga gttcatgggg aagaaagtca agcccgccga
     1801 cacgctcggc atcctcgagg cgcttgtcga ggggaaggcg gagttcgtgc cgttcggccc
     1861 cttcagcgat atggagacga tggcgctgga tcgcttcccg atcgacttca cggatgagca
     1921 gtaccgaagg gagctcttct cgcggatgag ggacaggctt gcgttcgtca cgtcccgaga
     1981 gacggagaag ggcggtatgg accgcctgcc acccgaagcg gtggccgcgc tgcggaaggc
     2041 gctcgacgag atggggtatc ccgtccagga gtaacgtttc ggagccgggg cccgtccccg
     2101 gctcatcgtt ttctgtatga gggagaatag tactcggtcg tcacctcctc gccgcgaaga
     2161 agccgcgcgc gggagatgat cctccgtctg cagacccatt cgaatccttc gcgtgcgctc
     2221 cgttcgtccg tcctgtcctt ctccatcccc tcggggtctt tcgccgctcc cggccacgac
     2281 cgccactccc ccaggagagg ctcctggtag agcaccagcc atgaaaaatc attccagaga
     2341 ccaaggattc tgaagcgctt tcgcccctct ccgtcgacgt ggatgagaat actcagctcg
     2401 tcgcggaagc gcgtggccgt tcccgagtac tttttgtccg cgctcttccc ggagaaggac
     2461 gccgtctgtc cgttgcggac gaccccctgc gaggcgcacc attcgcgcag cgttgccatc
     2521 agcgcgtcga agtccagagg aatgccgttc gacgcggcga atgcctgcgc gaatttccgt
     2581 tcgagcagat attcgccacg ccggtgcgag ggggcgcaga gcagatggag acgcagcggg
     2641 aaaagaaacg cttccgtcat gaatcctccc cggagatatt ctcgcccctt tattgcgggg
     2701 cgctctctgc tataatcata cctcaaacaa atttgtacgc ggaggaaggc tcttatggaa
     2761 aaaaatctga acaactcgca cgcgatgggg actgattcga tcactcgtct catgtggcga
     2821 ttttcgcttc ccgccatcgt cggtatgctc gccaacgcgc tctacaacat cgtcgaccgg
     2881 ctcttcgtcg gacagacggt gggcgccgcg ggcatcggcg cgatcagcgt ggtcttcccc
     2941 tttatgatac ttgtgatctc cttcgatctg ctggtgggcg tcggcgcagg ctcgctcatc
     3001 tccatctccc tcggcgagaa gcggagagcc agagcggaac gggcgatggg gaacgcgatc
     3061 atgctccttg taggcggcgg aatccttctg gttcttttgg gcgtctcgtt cacagagtct
     3121 attcttgaac tttcgggcgc cagcgcaact ttgatgcccc tctgtctgga gtacatgaac
     3181 attgccgtat ggggcgtctt cttctccacg cttgccttcg ggctcaactt cttcatcagg
     3241 gccgagggaa acccgcgcta cgccatgttc acgctcatca tcggcgcggg agcgaacgtc
     3301 cttctcgacg cgctcttcat cctggtattc gacatgggcg tcggaggggc cgctctcgcg
     3361 acgctgtttt cacagatgat ctccgccctg tgggctgcgc tgtactacat ccgcagaatg
     3421 gggacgctcc ggttcaggat gaagaacctt gcccccgact gggtgatcac ccgcaggatt
     3481 cttgcaatcg gttcggcgcc tgcgctcacc gaggtcagtt ttacgtacgt gctcgccctc
     3541 ttcaaccggc tcctgagctt ttacggaggc gatctggcaa tctcggcgat gggcatcttc
     3601 ttcagccttg acagtctctt ttttctgccg gtcctcggcc ttgcgggcgg cgtccagccg
     3661 atcatcagct acaactacgg cgcaaaggac tacgaccggg tcatccgctc cgtcctggct
     3721 gcgatcaagg tcggggccct cttcttcacg gtgagcttca cgctcgtcat gctctttccc
     3781 gcgccgctca tccgcatttt caactcggac gaccccgaac ttctggccct tggcgtgcgc
     3841 ggaatgcgac ttgcctacag cggcgtcatc tttgcctcgg tgagcctcat agcgtcgcat
     3901 acctttcagg cgatcggcaa ggcgaagacg gggctctttc tgacgctctc aaggcacttt
     3961 atcttcattc tcgccccgct gtactttctg ccgccgattc tcggaatgga cggcatctgg
     4021 ctgtcgctgc cgctctccga ccttggaggc gcgctcaccg gaacgtggtt cctgcggcgg
     4081 gagttccgta tcatgaggaa tgcagaacgt ctgcagcaga tttccgctcc cggcgagtgc
     4141 agcgtctccc cggattgagt ataataccgg tggagcttta tttcacaaat gagtgaggag
     4201 tgaagggagt gcgcgcatcc gtacatccgt ttccagaagg ggtttgcgag gtctctacgg
     4261 aggagggggc cgccctcttt cttatagacc tgccgattgc tcttccggga tttgatcatc
     4321 atctgtcgtc gtggctgctt cgtgatttca agagagggcg caacatcctc atcgacactg
     4381 ggccgtcggc ttcggtccct ttcttggagc gttctttgcg ctctctcggt gtggaacgac
     4441 tcgatctcgt gctgctaacc catattcatg cggatcacgc gggaggggcg ggacgtctcc
     4501 ttgagcgctt cccggaggcg agggtgctgg cgcccgagag gggaagaacg cacctcgtcc
     4561 acccctcgag gcttgccgag ggaacgcgcg cgacgctggg cgcggcgatg gcggacgcct
     4621 tcggtccgat tcttcccgtt cagaaggagc gcctgatcga ctcctctgac cctttgttgg
     4681 aagggtttac ggtgctcgag actccggggc acgcgtcgca ccactcgtcg tacgtcttcc
     4741 acgcggattt cgggaagatc ctctttcccg gggaggcggc gggcgtgggg cttggcgagg
     4801 cggtccgctc cctctggacg gcgcccggca tgccgaatgt ctccgcgccc tatcgttttc
     4861 ccgcaacccc gccgcgcctg gacggggaag cgctggagcg ttccatggaa cttctttcgg
     4921 cggagagcgg ggcgaagtat ctctgcttca gtcactacgg ctggaccacg gaggttgccg
     4981 aaacactgaa tctggccgcc gtgcagttcc gcgactggtg tgagagggcg cgacggtgtg
     5041 ctgcggcgga cgaagaggat gcggtggcgc tgctcttcga cgagacgcaa cgggaggacc
     5101 cgctgctcgg acttctttcc tctttgagga cggagcagcg tcaaaaggag gagttcttca
     5161 tcaaaaacag cctgaggggg attgtccgat gggtacgatc ataacattcc ggcccggcaa
     5221 gcgcggccgt acattcccag gagggtatcg ccgcatactt tgggaagagg ggggatcgta
     5281 gtgacggaac agtccgggaa gcttggattc gaccgctatc tcgtcggaga ggggggcaaa
     5341 cggctctttc tcttcctttt cggaggactt ctttgtacag taggcctgca ctccagcccg
     5401 ttcgctgaaa tcctgccggg gttgaagctt attatcttcg aacccgatta tctcgtctcc
     5461 gattacttcc atgtaggggg acgcggagct tcgtttctca acagcggaat tctcacgctg
     5521 ctcttcacgg caatccttgt gtactacaaa atccacatac gcggtatttc catcgcggcg
     5581 gtcttcaccg tagcagggtt tgccttcttc ggaaaaaatc tgctcaacgt ctggtttatc
     5641 ctcggaggcg tgtggctcta cgcatggtat cggaaggagt ctttcctgaa gttcatctac
     5701 atcgcttttt tcggcacagc cctcgcgccg cttgtgagcc agctgatgtt cggtctgtac
     5761 ctccccgcgc cttttcgaat agccgccggc gcgatcgcgg ggctttcagc cgggttcatt
     5821 cttccttcat tggcggcgtc cttcctttcc gtgcacaagg ggtacaacct gtacaacgtc
     5881 ggatttacag cagggatggt gggaacgctt tatgtctcgc tctttcgctc gcacggtttc
     5941 gtaccggagg cgagggtgct ctggtccacc gggaacaacg ggctgctcct ccccatgctt
     6001 ctcgggatgt ttctgacgat gatcctcgcc gggtatttat taaacggtag atccttcaaa
     6061 ggcgtttcgt cgatttgggc gcatccggga cgactgcttg ccgacttcgt cgatatcttc
     6121 gatttcccgc cgacgctgat caacatgggg gtgaacggcc tgctcgtgac cggctatctg
     6181 ttggctgtag gcggcgattt caacggtccc acgctcggag gcatctttac cgtcgtcggt
     6241 ttcagcgcga tggggaaaac gccgaggaac atcgcgccta ttatcgcggg agtcgtcctc
     6301 ggaagtctga cgaagcactg gagtctttcc gacccggcaa tacagcttgc ggcgctcttc
     6361 ggaacgacgc tcgcgcctat tgcgggcgaa ttcggctgga aagcgggaat actggccggg
     6421 tacgtccact cgtccgtggt gctgtatgtc ggcgtactgc acgccggctt caacttgtac
     6481 aacaacggct tcgccggggg actcgtcgcg gctatcctcg tccccctcat cgaaaccttc
     6541 agagggagag aaaaggaatg aaatacgaac gtcatgtcgt ctcgcgcatc gtgagcgagt
     6601 taatgaactt cttcttctcg atgggagcgc gcgacttttc gtcgcatgtc atccggaacg
     6661 aagacaggtt tgaaatatgc gttgaatcgg actacgcggg caacccgacg acaaagatcc
     6721 gggagatgac ccggctgctc agaatgccgc gcgcccgcga aatggaggag tactcctggt
     6781 cgctctccgg agacatcagc acgggacagg agatctatct cgtggggatc atgacggatg
     6841 acgtgaccgt ggaacacgac gaagaggcgg ggcgggtccg catctgtctc acccgtcttc
     6901 tggagtgacc tgaaaggggg aggaaagtat gccgaagctt gaat
//
LOCUS       DAOTIL010000029         6937 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminobacteriaceae bacterium isolate AMR_MDS_3026
            NODE_12972_length_6937_cov_3.518018, whole genome shotgun sequence.
ACCESSION   DAOTIL010000029 DAOTIL010000000
VERSION     DAOTIL010000029.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868050
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminobacteriaceae bacterium (wastewater metagenome)
  ORGANISM  Aminobacteriaceae bacterium
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae.
REFERENCE   1  (bases 1 to 6937)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6937)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:19:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,224
            CDSs (total)                      :: 3,198
            Genes (coding)                    :: 3,184
            CDSs (with protein)               :: 3,184
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6937
                     /organism="Aminobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12972_length_6937_cov_3.518018"
                     /isolate="AMR_MDS_3026"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:3034609"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            200..1618
                     /locus_tag="P5201_01140"
     CDS             200..1618
                     /locus_tag="P5201_01140"
                     /EC_number="3.4.21.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005661849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0006950 - response to stress [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Do family serine endopeptidase"
                     /protein_id="HRV97167.1"
                     /translation="MKGTKITSAFFGSVKVLFVLLLLVVPFLLTADRAFAQDPFTGNP
                     VAKIARELSPAVVNIDVETMVTRSVHPFADDPFFRQFFGEEQRRFSRTVPMKGRGSGF
                     IVSKDGHIITNNHVIDGADKITVTFSDGRTFEAKVLGKDPTFDLAVIKIDGADLPFLE
                     LGDSDATEVGEWVVAIGNPFGLEHTVTVGVVSAKNRSIHAGNVNFDGFLQTDAAINPG
                     NSGGPLLNLEGKVVGINTAIVPFAQGIGFAVPVNMAKQVMDDLIEFGRVKRAWLGVYI
                     QPLTKEFAEAYGVKEENGAVVSDVLRDSPAEKAGLQRGDVILEVDGSGVKNHQEFVMK
                     IRQKLAGDKVTLTLIRQKKNIKVDIPLGEMPGNEKQAASRTGGSKMLTSIGLEAGEMT
                     DDIRKQNNLRKDEGVLITAVEDGSMAQRAGIRENDVLLEVNGVRLSGLSDLDKADVKG
                     NSVVLLIMRDSRSFFVSIRTGQ"
     gene            complement(1694..2371)
                     /locus_tag="P5201_01145"
     CDS             complement(1694..2371)
                     /locus_tag="P5201_01145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013048100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HRV97168.1"
                     /translation="MSVLIIEDNRDLVQVLAEVLNENGFSVESAHTGEEGLEKALLSR
                     FDCIILDIVLPGINGFQVVEEYRKEDGSSPVLILSARDSVEDKVEGFNRGADDYMVKP
                     FDSRELIVRVRSLIRRAADKQSPLLACGRLTLDPVTRECRVDGTFVNLRRREFDILET
                     MLRHANQVFTRERLVASVWKKEYDGRSNVVDVHVKYLRDKLRAHGFDTAIETIRGVGY
                     KIRCSEE"
     gene            2631..2897
                     /gene="rpsO"
                     /locus_tag="P5201_01150"
     CDS             2631..2897
                     /gene="rpsO"
                     /locus_tag="P5201_01150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012869466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S15"
                     /protein_id="HRV97169.1"
                     /translation="MLQGEKKQGIITEFQVHTTDTGSPEVQVAMLTQRIRELTEHLKI
                     HTKDFHSRRGLLKMVGKRRRLLRYLKDKDFNRYRTLIERLGLRH"
     gene            3012..5297
                     /locus_tag="P5201_01155"
     CDS             3012..5297
                     /locus_tag="P5201_01155"
                     /EC_number="2.7.7.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009163752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004654 - polyribonucleotide
                     nucleotidyltransferase activity [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA];
                     GO_process: GO:0006402 - mRNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyribonucleotide nucleotidyltransferase"
                     /protein_id="HRV97170.1"
                     /translation="MEMTFSVEIGGRPLEFSTGKVAKQAGGAVVAAHGETTVLTTACI
                     TDKPRTGIDFFPLLVDFEERFYSAGKIPGGFIKREGRPSETAILSCRMVDRSIRSLFD
                     ETMRHDVHVVSTVLSVDQVNPPNVLAINAASAALTISDIPWGGPVGAVRIGRLGDEFV
                     VNPTEEQMLESTLDLLVAGHLDGVTMVECGSKEIAEDLLVDALELAQNEIKKIVGLFN
                     EMRAAVGREKLVLPSAPSFPDIDAWAAENLTGEIRDAVKIHEKKPRGDAISAARNKLQ
                     EHFQAQYPESGDYVAGLIDEMVKKTMRSLIVDERTRADGRRMDELRKITCETGVLPRV
                     HGSALFTRGETQALVVTTLGMMGVDDQILDGLKQDEPAKRFILHYNFPPFSVGEVRPM
                     RGPGRREIGHGALAERALRSMIPDEADFPYVMRVVSDILESNGSSSQASICGGSLALM
                     SAGVPMKKHVAGIAMGLIKEGEKVAVLTDIQGLEDHFGDMDFKVAGTRDGVTALQMDN
                     KAGGITRAILEQALSQARAARMQILDSMEAAIPVPAELSANAPRIFMTTIDPEKIRDV
                     IGPGGKVIRGITQKTGVKINVEDSGEIYIGGSSQEKVDEALSIIRGLTKDLEAGEVYH
                     GTVTRLMTFGVFIECLPGKEGLLHVSEVSTHRIPKVEDVFKVGDKVLVMVKEIDDMGR
                     TNLTRRRILENESRIAEEGLSEVLPAEQERETVIAALAEANKNNPQPERERPRDGGGR
                     GDRRPGGGGSGPRRHSSGGRS"
     gene            5304..5747
                     /gene="dut"
                     /locus_tag="P5201_01160"
     CDS             5304..5747
                     /gene="dut"
                     /locus_tag="P5201_01160"
                     /EC_number="3.6.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005661858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004170 - dUTP diphosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006226 - dUMP biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0046081 - dUTP catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dUTP diphosphatase"
                     /protein_id="HRV97171.1"
                     /translation="MPVAVKILREGRAQEFPLPEYATESAAGADLRSAEDMLLLPGER
                     TSVGTGIRIELPEGYEAQIRPRSGLAIRHGVTILNAPGTIDSDYRGEIRILLVNLGKD
                     PFTIVAGDRIAQLVVAPVARVEWREDETLGLTGRGEGGFGSTGKS"
     gene            6067..>6937
                     /locus_tag="P5201_01165"
     CDS             6067..>6937
                     /locus_tag="P5201_01165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015556512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine--tRNA ligase"
                     /protein_id="HRV97172.1"
                     /translation="MMQFTGNNGKQAELASGTAGDVLDALGMKKGAIAAKIDGCAVDL
                     SRVVERGGEAAPILPESDEGLDILRHSAAHLMAQAVAQMYPGTRYGVGPAIKDGFYYD
                     MEIPGGITEEDLPKIEKEMHRIAKRSVPVERRVLSKAEALEWFKAKNDPYKVEIISEL
                     EDETVTLYVQGEYADLCRGPHVPNTSYVRHFKLLSVAGAYWRGDEKNIMLTRVYGTAF
                     GDEESLAAYIHRMEEAKNRDHRRLGKELDLFSLQAEGPGFPFFHPKGMVILNNLMDFW
                     KKEHTKRGYCEIRT"
BASE COUNT         1567 a         1911 c         2088 g         1371 t
ORIGIN      
        1 atcgggatgc agacgaaccg ctctgttctc ttcgttgtgc tcggaataag catgctctgg
       61 ctcgggtatt ccgtattcta cgagaaaaaa tgtatacgaa gttgatatga aatgcgtatt
      121 catggatagg taaaaaaaac gatgtatcca gccgccgggc gcatacaata aaagcatctt
      181 gattcggagg tgacacacca tgaaaggaac aaaaattacg agtgcgttct tcggttccgt
      241 gaaagttttg tttgtgcttc ttctgcttgt cgtgcccttt ttgctgacgg cggatcgggc
      301 attcgcgcaa gatccgttta ccgggaaccc tgtcgccaaa atagcaaggg agctttctcc
      361 cgcggtggtc aatatcgatg tggagacgat ggtcacccgt tccgtccatc cgttcgcgga
      421 cgacccgttt ttccgccagt tcttcggtga ggagcagcgc cgtttctccc gtaccgttcc
      481 catgaaaggg agaggctccg ggttcatcgt gtcgaaggac ggacatatca tcacgaataa
      541 ccacgtcatc gacggcgccg acaagataac ggtgaccttc tcggacggcc gtacgttcga
      601 ggcgaaggta ttgggcaagg atcctacatt cgatcttgcc gtcatcaaga tcgacggtgc
      661 agacctcccg tttcttgaac tcggcgattc ggacgcgacc gaggtcggcg agtgggtagt
      721 cgcgatagga aatccgttcg gacttgaaca tacggttacc gtcggtgtcg tctcggcgaa
      781 gaaccggagc attcatgccg gaaacgtgaa cttcgacggg ttccttcaga cggatgccgc
      841 gatcaacccc ggcaatagcg gcggtcctct tctgaacctg gaagggaagg tcgtcggaat
      901 caacaccgcc atcgttccgt tcgcccaggg tatcggcttc gctgtgccgg tgaatatggc
      961 gaagcaggtc atggacgatc ttattgagtt cggccgggtg aagagagcat ggctcggcgt
     1021 gtacattcaa ccgctgacca aggagtttgc cgaggcatac ggtgtgaagg aagagaacgg
     1081 ggccgttgtc agcgacgtgc ttcgcgactc acccgccgaa aaagccgggc tccagcgggg
     1141 cgatgtcatc cttgaggttg acggttccgg cgttaagaat caccaggagt tcgtgatgaa
     1201 gattcggcag aaactcgccg gagacaaagt taccctgacg cttatccgcc agaagaagaa
     1261 tatcaaggtg gatatccccc tcggcgagat gcccggcaac gaaaaacagg ccgcttcccg
     1321 caccggcgga tcgaagatgc tcacctctat cggattggaa gccggcgaga tgacggatga
     1381 tatccggaag cagaataacc ttcgcaagga tgagggtgtg ctgatcaccg ccgtcgagga
     1441 cggctccatg gcgcagagag cgggtatccg cgagaacgac gtgcttctgg aagtgaacgg
     1501 cgtccgtctt tccggcctct cggatctcga caaggcggat gtcaagggca attccgttgt
     1561 gctgctgatt atgagggatt cgagatcctt cttcgtctcc atacggacag ggcaataaag
     1621 aagagtcagt aaaagaagaa gagcgaagcg cccctgcacg ggcgcttcgc tcttcttctg
     1681 gagtaggttc ctcctactct tcggaacaac gaatcttgta tcccacacct cgtatcgttt
     1741 cgatggcagt atcaaatccg tgagccctca gcttgtccct gagatacttg acatggacgt
     1801 cgacaacgtt gcttctgccg tcgtattctt ttttccacac ggatgcgaca agacgttccc
     1861 ttgtgaacac ctggttggcg tgccggagca ttgtttcaag aatatcgaac tccctgcgtc
     1921 tgaggttgac gaaagttccg tccaccctgc actcccgcgt caccggatcg agcgtgagcc
     1981 gaccgcaggc aagcagcggc gactgtttgt cggcggctct ccggatgagc gatcgtaccc
     2041 gcacgatgag ttctcttgag tcgaacggtt tcaccatgta gtcgtcggct ccgcggttga
     2101 acccctccac cttgtcctcc accgagtcgc gggcggacaa aatgagtacc ggcgaacttc
     2161 cgtcttcctt tctgtattcc tccacgacct gaaacccgtt gatccccggg aggacgatat
     2221 ccaaaatgat acagtcgaat cttgaaagca gcgctttttc aagcccttct tcccctgtgt
     2281 gcgcgctctc aacggaaaaa ccgttttcgt tgagcacttc ggcgagcact tgcactagat
     2341 ctctgttgtc ctcgatgatg agaacggaca cgttgcttcc cccctaatga aaaaactctg
     2401 gtaaaataat caaaaagatt aaagcacatc cagaattaaa agtcgagtgc aagatgagat
     2461 cactttttct ttttctgaaa tccagctgaa atatgccctc ttcccgctgg tcgtgcgtga
     2521 tgatttgctt tattttctgc aagatgatag tattactggc gacccttacc cggatttgcg
     2581 catagagcgc tcttctgagt tacgggattt tgtctacgag gtgaagaaac atgttgcagg
     2641 gagagaagaa acagggaatt attacggagt ttcaagtgca cacgaccgac accgggtcgc
     2701 ccgaggttca ggtggcgatg ctcacgcagc gcattcgcga gcttaccgaa catctcaaga
     2761 ttcacacgaa ggacttccat tctcgccgtg gtcttctgaa gatggtcggc aaacgccgcc
     2821 gacttctccg gtatctgaag gacaaggatt tcaaccggta cagaacgctt atcgagagac
     2881 tcggtctgcg tcactaaaaa aggtcgattt ccggtgcgag ggcgaaaagt cctcgcactt
     2941 tttttgttcg attgtggtat tcttactccg catacaataa aaagagctga attttgagga
     3001 ggaagaagtg tatggaaatg acgttttcag ttgaaattgg aggccgtcct ctggagtttt
     3061 ccaccggaaa agtggcaaag caggcaggcg gagccgttgt ggcggcacat ggcgaaacga
     3121 cggtgcttac cacagcctgc atcaccgaca aaccgcgcac cgggatagat tttttcccgc
     3181 tgcttgttga ttttgaagag agattttact ccgccgggaa gattcccgga gggtttatta
     3241 agcgcgaagg tcgcccctcc gaaacggcca ttttgagctg ccggatggtc gaccgttcca
     3301 tccgctcgct ttttgacgag acgatgcgcc acgacgtcca tgtggtttcg acggtgctct
     3361 cggtcgacca ggtgaacccg cccaacgtgc tcgcaatcaa cgccgcatcc gccgcgctca
     3421 ccatctcgga catcccgtgg ggaggcccgg ttggagccgt aaggatcggc cgtctcggcg
     3481 acgaattcgt ggtgaacccc accgaggagc agatgctcga atccaccctc gaccttctgg
     3541 tcgcaggaca tctcgacgga gtgacgatgg tcgagtgcgg ttcgaaggag atcgcggaag
     3601 acctcctggt cgacgccctt gagctggcgc agaacgagat caagaagatc gtcggcctct
     3661 tcaacgagat gcgcgccgct gtaggacgcg agaagcttgt acttccgtca gctccctcct
     3721 tccccgacat cgacgcatgg gcggcggaga accttaccgg agagattcgc gacgcggtga
     3781 aaattcacga gaaaaaaccc cgcggcgacg cgatatccgc agccagaaac aagctgcagg
     3841 agcattttca ggcgcaatac cccgagagcg gcgactatgt cgcaggactc atcgacgaaa
     3901 tggtaaagaa gacgatgcgt tcgctcatcg tcgacgaaag aacccgggct gacggtcgcc
     3961 ggatggacga gctcaggaag atcacctgcg agacgggcgt tcttccgaga gtgcacggct
     4021 ccgcgctctt tacccgcggc gagacgcagg cgctcgttgt gacgacgctc ggcatgatgg
     4081 gtgtggacga ccagatactc gacggactga aacaggacga gcccgccaag cggttcattc
     4141 tgcactataa cttccccccg ttctccgtcg gagaggttcg tcccatgaga ggaccgggac
     4201 ggcgcgagat agggcacggc gcgctcgcgg agagggcgct tcgctccatg ataccggacg
     4261 aagccgactt cccctacgtg atgcgcgtgg tctcggatat cctcgagtcc aacggctcca
     4321 gctcccaggc ctctatctgc ggcggcagcc tcgctctgat gagcgcgggc gttccgatga
     4381 agaagcacgt cgctggaatc gcgatggggc tcatcaagga aggcgagaag gttgcggtgc
     4441 ttaccgatat tcagggtctt gaagaccact tcggcgacat ggacttcaag gttgccggaa
     4501 cccgcgacgg cgtgaccgct ctccagatgg acaacaaggc cggcgggatc accagggcga
     4561 ttcttgagca ggcgctctcc caggcccgtg cggcgaggat gcagatcctc gacagcatgg
     4621 aggccgcgat ccccgttccc gcggagcttt ccgcgaacgc gcccagaatc ttcatgacca
     4681 ctatcgaccc cgaaaaaatc cgcgacgtca tcggccccgg cggcaaggtt atccgcggca
     4741 ttacgcagaa gacgggcgtc aaaatcaacg tcgaggacag cggcgaaatc tacatcggcg
     4801 gctcctcgca ggaaaaggtc gacgaggcgc tctccatcat ccgcggcctc acgaaggacc
     4861 ttgaggcagg agaggtctac cacggcaccg tgacccggct catgaccttc ggcgtcttca
     4921 tcgagtgcct tccgggcaag gaagggctgc ttcatgtgag cgaggtcagc acccaccgca
     4981 tcccgaaggt ggaggatgtc ttcaaggtag gcgacaaggt gctcgtcatg gtgaaggaga
     5041 tagacgacat ggggcgcacc aacctgacgc gcagacgcat cctcgaaaac gagagccgta
     5101 tcgccgaaga aggtctctcg gaggttctgc ccgccgagca ggagcgagag accgtgattg
     5161 ccgcgcttgc ggaggcgaac aagaacaatc ctcagccgga gcgggaacgc ccccgggacg
     5221 gcggcggaag aggcgaccgc aggccgggcg gcggcggaag cggtccccgc aggcactcat
     5281 cgggaggaag gtcgtagcct ccaatgccgg tggctgtaaa aattctcagg gaaggaagag
     5341 cgcaagagtt ccctcttcct gaatacgcaa cggaaagcgc tgcaggcgcg gatcttcgct
     5401 cggcggagga catgctcctc ctgccggggg agcggacgag cgtcggcacc ggcatccgga
     5461 tagagcttcc cgaagggtac gaagcgcaga ttcgacccag gagcggtctg gctattcgtc
     5521 acggtgtgac gatcctgaac gctcccggaa cgatcgacag cgattacagg ggggagatca
     5581 ggatcctcct ggtgaacctc ggaaaagacc ccttcaccat cgtcgcgggc gacagaatcg
     5641 cgcagcttgt cgtcgctccc gtcgccagag ttgaatggcg cgaggatgaa acgctcgggc
     5701 tgacgggacg gggagagggc ggtttcggga gcaccggaaa gagttgacag attccccttt
     5761 taggggtata agttcagaaa agttttcttt cctgcgatga gagggagagt agggacgatt
     5821 cgtccggaca gagagggagg cacgcggctg aaaggcctcc tccggattcg tcatcgaaca
     5881 ccgcccttga gctgacgccg aaatacccct cgggacaagg cgtctgggga tccgccccga
     5941 acagcggaca cgagcgccgc gcaaaagcag tacgcgcgga aatggagtgg aaccgcgaca
     6001 tgcccacgtc gtctccataa tggagatgcg tgggcatttt tttatcgaaa acgaggaggt
     6061 aggagtatga tgcagtttac agggaacaac ggcaaacagg cggagttggc ttccggcacg
     6121 gcgggcgatg tgctcgacgc gctggggatg aagaagggcg cgatagcggc gaagatcgac
     6181 ggctgtgcgg tggatctctc gcgcgtcgtc gaaaggggcg gcgaggctgc cccgatactt
     6241 ccggagtcgg acgaggggct cgacattctg cggcactccg cagcgcattt gatggcgcag
     6301 gccgtcgccc agatgtaccc gggaacgcgc tacggcgtcg gcccggcgat caaggacggt
     6361 ttttattacg acatggagat ccccggaggc atcaccgagg aggatctgcc gaagatcgaa
     6421 aaggagatgc accggatcgc caagcgctcc gttcccgtgg aacggcgggt gctgagcaaa
     6481 gccgaggcgc ttgaatggtt caaggcgaag aacgacccgt acaaggtaga gatcatctcc
     6541 gagctggaag atgagacggt cacgctctac gttcagggcg aatacgcgga tctctgccgg
     6601 gggccgcacg tccccaatac ctcctatgtc cgtcacttca agctgctctc cgtggcgggg
     6661 gcgtactggc ggggcgacga gaagaacatc atgctcacca gagtctacgg aacggccttc
     6721 ggcgatgagg agtccttggc ggcatacatc cacaggatgg aggaggcgaa gaaccgcgac
     6781 cacagacgtc tcggcaagga gctcgacctc ttcagcctcc aggccgaggg gcccggtttc
     6841 ccgttctttc accccaaggg gatggtcatt ctcaataacc tgatggactt ctggaagaag
     6901 gagcatacaa agagagggta ctgcgagatc cgcaccc
//
LOCUS       DAOTIL010000030         6914 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminobacteriaceae bacterium isolate AMR_MDS_3026
            NODE_13022_length_6914_cov_3.773582, whole genome shotgun sequence.
ACCESSION   DAOTIL010000030 DAOTIL010000000
VERSION     DAOTIL010000030.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868050
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminobacteriaceae bacterium (wastewater metagenome)
  ORGANISM  Aminobacteriaceae bacterium
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae.
REFERENCE   1  (bases 1 to 6914)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6914)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:19:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,224
            CDSs (total)                      :: 3,198
            Genes (coding)                    :: 3,184
            CDSs (with protein)               :: 3,184
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6914
                     /organism="Aminobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13022_length_6914_cov_3.773582"
                     /isolate="AMR_MDS_3026"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:3034609"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..511
                     /locus_tag="P5201_01170"
     CDS             <1..511
                     /locus_tag="P5201_01170"
                     /inference="COORDINATES: protein motif:HMM:NF020479.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF1850 domain-containing protein"
                     /protein_id="HRV97173.1"
                     /translation="NVLDAIRKRAFSLLLFFSLIFLLCVPVESLTVRSSDGSSISVCP
                     VAPGDSFMLSFIHSVEKTPVESEFRVLSGKVRQWEERFLSHNAGLPTEAPPNGRFIMD
                     KDWMILRGGGLAVQSIRYRVGNEQFGRNYMVLPDGAKILLFEEYPGRILFFSAESTSF
                     LNYLFSQVF"
     gene            534..2486
                     /locus_tag="P5201_01175"
     CDS             534..2486
                     /locus_tag="P5201_01175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006583456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter permease"
                     /protein_id="HRV97174.1"
                     /translation="MAEVPEKDGVNGQNEIDLDALRRQFDTEARYRKLSGFPAILVTS
                     LAVCMSLFHLYTSGFGLMQAIIQAAVHLAFVLVLVFLLYPFSPKFSKTEIPWYDYLLA
                     ALGAFVTLYLVFEFDALITRAGLPTTMDLIVAFLGVAILLEATRRVTSPILPVIAIFF
                     LLYCYYGRYFPALFQHRGFNINRIVNHMYLGTEGIFGIPLGVSATFVFMFILFGSVLE
                     QTGMGKFIIDLAMALAGWATGGPAKVAVLSSGLMGSISGSSVANVCTTGMFTIPLMKS
                     VGYKAHFAGAVEAVASTGGQIMPPVMGAAAFIMAQFMGVPYIQVALAAIVPALLYYFA
                     VIVQVHFEATRLGLRGLPRDQLPPLLRLLRQKGHLLIPLVGIVYFLIGGYTPLKAAYN
                     GIIITFVVSWLNKETRLTLPRLIAALESGARSALGVACACATVGIIVGTATLTGLGLR
                     IASAIVTLAGGNLLLTLLLTMVACILLGAGLPTTANFIVTSTMAAPALFQLGVPPMAA
                     YMFVLYFGIAADLSPPVALAAYAGAGIAGAEPMKTGVTAVKLALAGFIVPFIYVFNPI
                     LVLVDYSFVPFALAVGTAMLGVVLLGMSTVGYFRGVLSLWKRALALAGALFLLIPGWQ
                     SDLFGIAVLVLLYILQSFSSRKAALG"
     gene            2582..5374
                     /gene="ileS"
                     /locus_tag="P5201_01180"
     CDS             2582..5374
                     /gene="ileS"
                     /locus_tag="P5201_01180"
                     /EC_number="6.1.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014163085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004822 - isoleucine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006428 - isoleucyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isoleucine--tRNA ligase"
                     /protein_id="HRV97175.1"
                     /translation="MSTDYKSTLSLPETAFPMRANLAKREPGFLDFWKERDIYGKMLK
                     KREGAPSFVLHDGPPYANGHIHIGTAFNKILKDFIPKYKAMKGFFSPYVPGWDTHGLP
                     IELHVIKSLGVSKDTIDPVLLREKCTEYALQFRDIQRDEFVRLGVLGDWENPYMTLRP
                     EYEAAQLGAFADLVGKGLVYKGKKPVYWCIDCQTALAAAEIEYWDETSPSIFVAYSLS
                     GAEALLPSLAGEDVNIIVWTTTPWTLPASMAVAIGPDHEYVFVRAGKKVYVFAKALAA
                     SVAKETGIVFEDELATVKGAALQQLHAVHPFYPDRTLPLLPADYVLLDTGTGCVHTAP
                     GHGVEDYETGVKFGIEVYNPVDDTGHFIPGTGLVDGLSLDEGSKKIFEILTANGRLLG
                     NSKIQHSYPHCWRCKKPVIFRSTDQWFVAVADFRNEALKTIENDVRWIPDWGKDRIFN
                     MVRDRSDWCISRQRIWGVPIPAFYCNDCGKPILTEDRIHAVQAFVKEQGCSAWWRMTP
                     GELLGNLCVCPHCKSTNLRKETDIMDVWFDSGTSHAAVLKIRPELKWPADMYLEGSDQ
                     HRGWFQTSLLTSVGMHGRAPYEQVLTHGFIMDGEGRKMSKSLGNVVAPQEVIKEYGAD
                     ILRLWVASTDYRNDVRISNTIIKNLSESYRRIRNTARYLLANLNDFAPADAVPFNEMV
                     EMDRWILMKLNSIIEKANSAYDNYEFHIPTFAIHQFCVNELSSFYLDASKDRMYADGK
                     NSLSRKSGQTAMWYVLSALTRMLAPVLSFTAEEIWQEMLKIDVSLPESVFLSDWPDVQ
                     EGIRNQEIEEKWDKALAVRGAVSRVLELARSGGIIGHALDARVEVEKQNTASVAATFS
                     EEDFKTFSIVSGFRWVDQVSQMSIVQTDEETGIRVGVDKALGEKCPRCWQYTESPDAE
                     GLCPRCSRVLHAQGDA"
     gene            5355..6380
                     /locus_tag="P5201_01185"
     CDS             5355..6380
                     /locus_tag="P5201_01185"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HRV97176.1"
                     /translation="MPKGMREEGRFPQELVPESSSPEADEEIAVTEAGGRSETLIVQE
                     VEAGSRLDLFLALRLGITRSFARKLLDSGNIDLSSAKKVKPGLKITPGLVVSATLPAP
                     ETMDLEPENVPFTVVHEDPCLIVVNKPAGVVVHPAPGNWRGTLVHGLLFRFDSWGVFN
                     NVHRPGIVHRLDGPTSGLLVVAKDQETLDGLQKQFRLREVEKRYLALVEGKIRHTSGR
                     IELPIGRSSSNRLRMTVDPDGKKAVTEFRVLWSRSGYTFVECHILTGRTHQIRVHMSH
                     IGHPLVGDALYGASGKDAEKLGRVFLHSWKLSFRHPVSRKTVWFTCPLPGELTGQLQD
                     ILSTNRD"
     gene            complement(6313..>6914)
                     /locus_tag="P5201_01190"
     CDS             complement(6313..>6914)
                     /locus_tag="P5201_01190"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRV97177.1"
                     /translation="KGKIDVAAVRKKIEAIEDGIRELEEQMGALESIDDPRLLAATAQ
                     DILEWLAPPKKTGAKKKKEELPPHIRFSYAGNEIFVGLNARGNRYVTFKAASPGDLWF
                     HIHEIPGAHIILKTPGEVYDDAKDSILLAASLAAWFSKARLSTKVQVDFTRRKNVRSI
                     PGSAIAHVTYTNPETVQISPGLWKEYPEAVRSIHLATDR"
BASE COUNT         1462 a         1786 c         1895 g         1771 t
ORIGIN      
        1 caatgtgctt gatgccattc gtaaacgtgc tttctcgctt ttgctctttt tttctcttat
       61 ttttttattg tgcgtgccag ttgagtcatt aacagttcgc tcttccgacg ggagttcaat
      121 ttctgtttgt cccgtcgccc ctggagattc ttttatgctg agtttcattc attcggttga
      181 aaagactcct gtggagagtg agttccgagt tctttcaggc aaggtgcgtc agtgggaaga
      241 acgttttctt tcccataacg cagggttgcc cacggaagct cctccaaacg gacgattcat
      301 catggacaag gattggatga ttctgagagg aggaggactt gccgtccaga gtatccggta
      361 tagggtcggc aacgagcaat tcggcaggaa ttatatggtc ctgccggacg gagcaaagat
      421 acttcttttt gaagaatacc cgggccgcat tcttttcttt tcagctgaat caacgtcttt
      481 cttgaattat cttttttcac aagtgttttg aatacgatca ggaggtggtt ttattggccg
      541 aagttcccga gaaggacgga gtgaacggac agaacgaaat tgatcttgac gctcttcggc
      601 gacagttcga cacagaggcc aggtacagga agctctcagg ctttcctgcc attcttgtta
      661 cttctctggc ggtatgtatg tcgctgtttc atttgtatac atcaggtttt ggtcttatgc
      721 aggctattat ccaggcggcc gtccacttgg catttgtcct cgtccttgtc ttcctgctct
      781 atcctttttc gccaaagttt tctaaaaccg aaattccctg gtatgactac ttgttagccg
      841 ccctaggggc attcgtcaca ctgtatctgg tttttgagtt cgatgctctt attacccggg
      901 cgggcttgcc gacgacaatg gatcttatcg ttgcattctt gggcgtcgct attcttctgg
      961 aagcaaccag acgagttacg agcccgattt tgcccgtcat cgccatcttc ttcctcctgt
     1021 attgctacta cggacgatat ttccccgcgc tcttccaaca caggggattc aacatcaacc
     1081 gtatagtgaa ccatatgtac ctcggaactg aaggaatttt cggtattccc ctcggtgtgt
     1141 ccgcgacatt tgtgttcatg ttcatccttt tcggttccgt gcttgaacag acaggaatgg
     1201 gcaagttcat aatcgacctt gccatggcgc ttgccggatg ggccacagga ggacctgcaa
     1261 aagtcgcggt actcagttcc ggactgatgg gttcgatttc tggttcttcc gtcgcgaacg
     1321 tctgtactac cggaatgttc accatcccat tgatgaaaag cgtcgggtat aaagcccact
     1381 tcgcaggagc ggtagaggcc gtcgcatcca ccggcggaca gattatgccg ccggttatgg
     1441 gggccgcggc gttcataatg gctcagttca tgggggttcc gtacatccag gttgctctgg
     1501 cggcaatcgt tccggcgtta ctgtactact ttgcggtcat cgtgcaggtt cactttgaag
     1561 ctacccgcct gggtctcaga gggcttccga gggatcagct tcctcctttg ctccgtcttc
     1621 tccgtcaaaa aggccatctg ctgatcccgc tcgtcggtat cgtctacttc cttatcggag
     1681 gatatactcc gctgaaagcg gcgtataacg gaatcatcat tacttttgtt gtttcctggt
     1741 tgaacaagga gactcgcctg acgcttccgc gtttaatcgc agctcttgag agcggcgccc
     1801 gttcagctct cggagttgcg tgcgcctgtg ccaccgttgg aatcatcgtg gggactgcca
     1861 cgcttacagg tcttggcttg cgtattgcca gcgcgattgt cactctggca ggaggtaatc
     1921 ttctcctgac gcttcttttg acaatggtag cctgtattct tctcggagcc ggtctgccga
     1981 caacggcgaa cttcattgtt acgagcacta tggcggctcc ggcactcttc caactcggcg
     2041 ttccgccaat ggcggcctac atgtttgttc tttacttcgg catagctgcc gacctcagtc
     2101 cacccgttgc cctcgccgca tacgcaggcg cgggaatagc gggagcagag ccgatgaaga
     2161 ctggtgtaac agctgtgaaa cttgccttgg ctggatttat tgttcccttt atctatgtct
     2221 tcaacccaat ccttgtactt gtggattata gctttgttcc gtttgcactt gctgttggaa
     2281 ctgcaatgct tggcgttgtt ttgttgggta tgtccactgt gggatatttt agaggagttc
     2341 tgagtttgtg gaagcgggct ctcgcccttg caggtgctct tttcctgctt attcccgggt
     2401 ggcagagcga tctgttcggt atagcggttc tggtgctgct ctatattttg cagtcattct
     2461 cctccagaaa agcagcccta ggataaggcc ggttgctaag aaatgtgtta gtatatagaa
     2521 gtatttttac cgtcccggtg ttttcaggga cggttttcct attccacaag ggggtacacg
     2581 catgagcacg gactacaaaa gcacgctctc gcttcccgaa acggccttcc cgatgcgggc
     2641 gaacctcgcg aagagagagc ctgggtttct tgatttctgg aaggaacggg acatctatgg
     2701 gaaaatgttg aaaaaacgtg aaggggctcc ttcatttgtt cttcacgacg ggcctcctta
     2761 tgcgaacggg catattcaca ttggaacggc gtttaataaa atcctcaagg acttcatccc
     2821 gaaatacaag gcgatgaaag ggttcttttc accgtatgtt cccggttggg atacgcacgg
     2881 cctgcctata gagctgcacg ttataaagag tcttggagtg tcaaaggata cgatcgatcc
     2941 agtacttttg cgcgagaaat gcactgaata tgcacttcag ttccgagata tacaaagaga
     3001 tgagtttgtg cgactcggcg tgcttggaga ctgggaaaac ccctacatga cgctgcgacc
     3061 ggagtatgag gcggcccagc tcggcgcctt tgctgatctt gtcgggaaag ggcttgtcta
     3121 caagggaaag aagcccgtct actggtgcat cgactgccag acggctctgg cggcggcgga
     3181 gattgaatac tgggatgaga cttccccttc aatcttcgtg gcttactccc tgtcgggtgc
     3241 ggaggcgctt ctgccgtcgc tcgccggcga agacgtcaat atcattgtat ggacgacaac
     3301 gccttggacg ctccccgcga gcatggctgt cgctattgga cccgatcacg agtatgtttt
     3361 cgttcgtgca gggaagaaag tgtacgtctt tgccaaggcc cttgccgcga gcgtggcgaa
     3421 ggagaccggt attgtcttcg aagacgaact ggctactgtg aagggagctg cgctgcaaca
     3481 gttgcacgcg gtgcacccgt tctaccccga ccgtactctt cctcttcttc cagctgacta
     3541 tgtccttctg gacaccggta ccggatgtgt gcataccgcc ccgggacacg gcgtggaaga
     3601 ctatgaaaca ggcgtgaagt tcggcataga ggtgtataac ccggtcgacg ataccggaca
     3661 cttcattcca ggcaccggac ttgtcgacgg gctttctctg gatgagggct caaaaaagat
     3721 cttcgaaatc ctgacagcga atgggcgtct tctgggaaat agcaagatcc agcactcgta
     3781 tccgcactgc tggcggtgta aaaaacctgt cattttccgt tcaacagatc agtggtttgt
     3841 tgctgtagcc gatttcagga acgaggcgct caagacgata gagaacgatg tccgctggat
     3901 ccccgactgg gggaaggacc gtatcttcaa catggtccgt gatcgttccg actggtgcat
     3961 cagccgccag cgaatctggg gagtgccgat accggctttt tactgtaacg actgcggaaa
     4021 acccatcctt accgaggaca ggattcacgc tgtccaggcg ttcgttaaag aacaagggtg
     4081 ttcggcatgg tggaggatga ctcccgggga gctgctcggc aatttatgcg tctgccccca
     4141 ctgcaaaagt acgaatctcc gtaaggagac ggacatcatg gatgtctggt tcgactccgg
     4201 aacgagtcat gccgccgtgc tgaaaatccg ccccgaattg aaatggcctg ccgatatgta
     4261 ccttgaagga agcgaccagc accgtggatg gttccagact tcgttgctga cttcggtcgg
     4321 gatgcacggc agggcgccgt acgaacaggt gctcacgcac ggtttcataa tggacggcga
     4381 aggacgcaaa atgtcgaagt ccctcggaaa cgttgttgcc ccgcaagagg tcatcaagga
     4441 gtacggcgcc gacattctcc gcctttgggt tgcctcgacg gattacagaa acgacgtccg
     4501 tatttccaac accattataa agaatttgag tgaatcgtac cgtcgaatcc gcaatacggc
     4561 caggtatctg ctggcgaacc tgaatgactt cgctccggcc gacgccgtcc cgttcaacga
     4621 aatggttgag atggaccgtt ggattctcat gaagctcaac tcgatcatcg agaaggcgaa
     4681 cagcgcatat gacaactatg aattccacat tcccacgttc gcaatccacc agttctgcgt
     4741 gaacgaactg agctctttct atctggatgc cagtaaggat cggatgtacg cggacggcaa
     4801 aaattccctt tcccgcaaga gcggacagac cgcgatgtgg tatgtgcttt ccgcccttac
     4861 acggatgctt gcgccggtgc tcagtttcac tgctgaggag atctggcagg agatgctaaa
     4921 aattgacgtt tcgcttcctg agagcgtctt cctctccgac tggccggatg tgcaagaagg
     4981 gatccgtaac caggagatcg aagagaaatg ggataaagcg ttggcggtcc gtggtgcggt
     5041 cagcagggtt ctcgagctcg caaggagcgg cggtattatc gggcatgctc ttgatgcgcg
     5101 ggttgaggtt gaaaagcaga acaccgcttc agttgccgca acattttctg aagaagattt
     5161 caagaccttc agcatcgtct ccggtttccg gtgggttgat caggtttcgc agatgagcat
     5221 cgtgcagacc gacgaagaaa caggaatcag ggttggtgtg gataaagcgc tcggggaaaa
     5281 gtgcccgcga tgctggcagt ataccgagag tcccgatgcg gaaggcctct gccccagatg
     5341 ctcgcgtgtg ctccatgccc aaggggatgc gtgaagaagg gcgtttcccg caagagttgg
     5401 ttcccgaatc cagctctccc gaggcggatg aggagatagc cgttactgaa gccgggggca
     5461 ggagcgagac gcttattgta caagaggtcg aggcggggag ccgtctcgac ctcttcttgg
     5521 cgcttcggct gggtattacg cgttcctttg cccggaaact gctcgattcg gggaatatcg
     5581 acctgtcttc agcgaagaaa gtgaaaccgg gtttgaagat aacgcccggc ctcgtagtca
     5641 gtgcgacgct gcccgctcct gagacaatgg acctggaacc ggagaacgtc cctttcacgg
     5701 tagtccacga ggatccatgt ctgatcgttg tcaataaacc cgcgggcgtc gtcgtgcacc
     5761 cggcccccgg taactggcgc ggcacgcttg tgcatggact gcttttccgc tttgattcat
     5821 ggggcgtctt caacaacgtc catcgtcccg gaatcgtgca tcgcctggac ggaccgactt
     5881 ccgggctgct cgtcgttgca aaggaccagg aaacgctcga cgggttgcaa aagcagtttc
     5941 gtctgcgaga agtagagaaa cgatacctgg ctttggtcga agggaaaatc cggcacacaa
     6001 gcggcagaat cgagctccct atcggccgtt cttcaagcaa caggctgcgt atgaccgtcg
     6061 atcccgacgg gaaaaaagcc gtaacggagt tccgggttct gtggagccgc agcgggtaca
     6121 cttttgtcga gtgtcatatt ctgacgggcc gaacgcatca gatacgggtt cacatgagcc
     6181 atatagggca tcctctggtc ggagacgccc tgtacggagc ttcgggaaaa gatgcggaaa
     6241 agctcgggag agtttttctg cactcatgga aactctcctt ccggcacccc gtttcccgaa
     6301 aaacggtttg gtttacctgt ccgttgccag gtgaattgac cggacagctt caggatattc
     6361 tttccacaaa ccgggactga tctgcactgt ctcaggattt gtgtatgtca cgtgtgctat
     6421 tgcgctccct ggtattgaac gtacgttctt gcgcctggtg aagtcaacct gtacttttgt
     6481 tgagagcctg gccttgctga accacgccgc caacgacgcg gccagaagaa ttgagtcctt
     6541 tgcatcatca tagacctctc ccggcgtttt caaaatgata tgggctcctg ggatctcgtg
     6601 aatatgaaac cacagatctc ccggagatgc ggccttgaac gtgacgtatc tgttcccacg
     6661 cgcattgagt ccgacaaaga tctcgttgcc tgcataagag aaccggatat ggggggggag
     6721 ctcctccttc tttttcttcg ccccggtctt tttcgggggc gcgagccact ccaggatatc
     6781 ctgcgccgtg gctgccagaa gccgcggatc atcaatcgac tcaagagcgc ccatctgttc
     6841 ttcgagttcg cgaatgccgt cctcgatcgc ctctattttt ttacgaacag cggctacatc
     6901 tatctttcct ttct
//
LOCUS       DAOTIL010000031         6725 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminobacteriaceae bacterium isolate AMR_MDS_3026
            NODE_13496_length_6725_cov_3.829685, whole genome shotgun sequence.
ACCESSION   DAOTIL010000031 DAOTIL010000000
VERSION     DAOTIL010000031.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868050
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminobacteriaceae bacterium (wastewater metagenome)
  ORGANISM  Aminobacteriaceae bacterium
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae.
REFERENCE   1  (bases 1 to 6725)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6725)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:19:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,224
            CDSs (total)                      :: 3,198
            Genes (coding)                    :: 3,184
            CDSs (with protein)               :: 3,184
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6725
                     /organism="Aminobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13496_length_6725_cov_3.829685"
                     /isolate="AMR_MDS_3026"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:3034609"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..986
                     /locus_tag="P5201_01195"
     CDS             <1..986
                     /locus_tag="P5201_01195"
                     /EC_number="3.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009201530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="sodium-translocating pyrophosphatase"
                     /protein_id="HRV97178.1"
                     /translation="IYTSGDYASVKHIAHASKTGPATVILEGVAVGMKSTVVPVILIC
                     AATLVSYYFAGLYGIACAAVGMLSITGMALSVDAYGPIADNAGGIAEMSHLPPEVRKI
                     TDKLDAIGNTTAAMGKGLAIGSAALTALALFAAYAHSVKLTAIDLNDPKVMVGLFLGG
                     MLPFLFSALSIQAVGRAAEAMIDEVRRQFREIPGIMEGTGRPEYEKCVDISTAAALKE
                     MIIPGLLAVLSPLAVGFGLGAQALGGLLAGSIVTGVMLAIFMSNAGGAWDNAKKYIEE
                     GNFGGKGTPEHAAAVVGDTVGDPFKDTAGPSLNILIKLMSVVALVLAPLFI"
     gene            1063..2808
                     /gene="dnaG"
                     /locus_tag="P5201_01200"
     CDS             1063..2808
                     /gene="dnaG"
                     /locus_tag="P5201_01200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013047988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003896 - DNA primase activity [Evidence
                     IEA];
                     GO_process: GO:0006269 - DNA replication, synthesis of RNA
                     primer [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA primase"
                     /protein_id="HRV97179.1"
                     /translation="MRSDDVRRVKDRLDIVEVIGDYVRLKKAGNNFLGLCPFHDEKTP
                     SFNVSPSRQTFHCFGCGQGGDVFSFIMEKENLSFREALETLAERAGVQLSSEGARTLR
                     ERSLFDIMERACSHFRSLLEKPEGEHAAAYLARRNLPREAWRNFELGWASPSWDALWK
                     ALSNEGVSFREAFDAGLVLEGKNGPYDRFRGRIIFPVRDISGRLLAFGGRLLDGEGAK
                     YINSPEGVLYSKRRSLYLLNRAKEAARSKDRLILVEGYMDAIRLHLNGFEETAASLGT
                     SLTEEQALLIRRLTDRCFICYDSDIAGQEASIRGMYVLQKAGLDVRVVEIPEGKDPDD
                     LLSLPDGKALFSKALEDARPLVLHHIHLRREMLRTPEKRRKASEDIISGIAMLSPVDA
                     SPYLPAVEAALGVLHHEFIPMLEEQRRKHSSQAREPVRDVPPDEETGEEQQPPDLVEA
                     ALCALLWRDCDLRRSADPVEIVSMLSDARLQTLASALLSASSAEDMEIYWHDIGDTFP
                     MKAIAAGGVYCDELERAHDPYTILVDILSVRKHKREYDLLKTKLSRGTAANDELMRFQ
                     KLAMILKSGKGKGTL"
     gene            2877..3155
                     /locus_tag="P5201_01205"
     CDS             2877..3155
                     /locus_tag="P5201_01205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006301335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding S4 domain-containing protein"
                     /protein_id="HRV97180.1"
                     /translation="MRLDKFLKLARLVKRRTVAQEMIEVGAVRINGRQCKPSAEVRVG
                     DSVEIAYPSRFLSLEVLCADEAALKRMPPEEAWSLREERRVDPSERPW"
     gene            3278..4561
                     /gene="eno"
                     /locus_tag="P5201_01210"
     CDS             3278..4561
                     /gene="eno"
                     /locus_tag="P5201_01210"
                     /EC_number="4.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009201525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004634 - phosphopyruvate hydratase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopyruvate hydratase"
                     /protein_id="HRV97181.1"
                     /translation="MSSIIGVHGREILDSRGNPTVEVEVWLDSGASGRAAVPSGASTG
                     TYEAVELRDGGPRYLGKGVLNAVNNVNEKIAPELMGFDADDQAEVDAALIELDGTPNK
                     GDLGANAVLGVSLAVARAAADDHDLPLWSWIGGLGPFSLPTPMMNVVNGGAHADNNVD
                     IQEFMLVPHGAETFPEALRMGVETYHTLKKAIHARGYSTAIGDEGGFAPDLKSNREAI
                     DLILEAVEKAGYAPGKDISIALDPAASEFFKDGKYHFAGEKKSFTPEEMVDYYEQLCK
                     DYPILSIEDGMAEDDWAGWSVMTKRLGSKIQIVGDDLFVTNPEKLSRGIEEGVANAVL
                     IKLNQIGSLSETLNVIALAKRYGYATVISHRSGETDDSFISDLAVATGAGQIKTGAPA
                     RVDRVAKYNQLLRIAEELDRAPYARLSQFRGNPNR"
     gene            4592..4972
                     /locus_tag="P5201_01215"
     CDS             4592..4972
                     /locus_tag="P5201_01215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008520174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HRV97182.1"
                     /translation="MKGIVSTTNAPAAVGPYSQAVKAGQFLFCSGQIPIDPATGQFVP
                     GGVAEQAKQVLNNVKAVLEAAGYSLADVIKATVFATSMNDFAAVNGVYATYFEKEPPA
                     RSFVEVSALPKGALVEIEVVAWKE"
     gene            5028..5663
                     /locus_tag="P5201_01220"
     CDS             5028..5663
                     /locus_tag="P5201_01220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009165503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="redox-sensing transcriptional repressor Rex"
                     /protein_id="HRV97183.1"
                     /translation="MKDTKIAEPTVERLVQYHRLLEQLAAEGQKVVSSREMGEMLSFK
                     ASQVRKDLSYFGEIGKRGVGYHVDRLFKHIGGILASPRLWSIGLAGVGKLGEALLGYQ
                     AFRNEKFQIKALFDVDPSKIGTHIQGTPCYSIEDAPRVLREEGIDVVIITVPTGAAQS
                     VADRVVSAGTVKGILNFSPVTLSVPDSVLLYSVDISIELEKLLFYLKHRER"
     gene            5755..6114
                     /gene="yajC"
                     /locus_tag="P5201_01225"
     CDS             5755..6114
                     /gene="yajC"
                     /locus_tag="P5201_01225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006301332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit YajC"
                     /protein_id="HRV97184.1"
                     /translation="MEQQCGQAAGGAGGQGSLMGMLFPLAIFVVIFYFFIIRPQKKRQ
                     RAQDELLSSLSRGDQVVTIGGFFGTIREVRDDSLVVEIADGVKVRLLKSAVASKRAPA
                     TQAPKEEPAREKEEEKN"
     gene            6305..>6725
                     /locus_tag="P5201_01230"
     CDS             6305..>6725
                     /locus_tag="P5201_01230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006301331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein translocase subunit SecD"
                     /protein_id="HRV97185.1"
                     /translation="MLRRDRWRLGIVAIVVLAALFSVFPIQGRINLGLDLKGGAHIVL
                     QAKDTADSKVTEDSIERLLAVLRNRVDQYGVAEPVIQREGTDRIIVDLPGIQDPDAAL
                     ELIGKTALLEFRQVVTATSPIPPSAERKNYDSDAEFER"
BASE COUNT         1335 a         1985 c         2124 g         1281 t
ORIGIN      
        1 agatctacac gtcgggagac tacgcctccg tgaagcacat agcgcatgcg tcgaagaccg
       61 gtcccgcaac ggtgattctg gaaggcgtgg cggtcggcat gaagtcgacg gtcgttcccg
      121 ttattctgat atgcgccgcg acgcttgtca gctactactt tgcaggcctc tacggcattg
      181 cctgcgcggc tgtcggtatg ctttcgatta caggaatggc cttgagtgtt gacgcctacg
      241 gtcccatcgc ggataacgcg ggcggcatcg ccgagatgag ccacctcccg ccggaggtcc
      301 gtaagatcac cgacaagctc gacgccatag gcaacacgac tgcggcgatg ggcaaaggac
      361 tggcgatcgg ctccgcggcg ctcaccgcgc tcgcactctt cgccgcgtac gcgcactccg
      421 tcaagctgac ggccatcgac ctgaacgacc cgaaggtcat ggtcggtctc ttcctcggcg
      481 gcatgcttcc gttcctcttc agcgcgcttt cgatccaggc ggtcggccgc gctgcggaag
      541 ccatgatcga cgaggtccgc cgtcagttcc gcgagatccc cggcatcatg gagggaacgg
      601 gacggcccga atatgagaag tgtgtcgaca tctccaccgc cgccgcgctg aaggaaatga
      661 tcatccccgg cctgctcgcg gtcctctccc cgctggccgt cggcttcggc ctcggcgccc
      721 aggcgctcgg cggcctgctc gccggttcca tcgtgacggg tgtgatgctc gccatcttca
      781 tgtccaacgc gggcggcgca tgggacaacg cgaagaagta tatcgaagag gggaacttcg
      841 ggggcaaagg gacgccggag cacgccgccg cggtcgtggg cgacacggtc ggcgacccgt
      901 tcaaggacac cgcgggcccg agcctcaaca tcctcatcaa gctgatgtcg gtcgtggcgc
      961 tcgtgctggc gccgctcttc atctaatccg aaaaagggag tacaatatct gcgagagggg
     1021 gagggcgacc tcccccttat tttttcttcg gaggcgccgc tcatgagatc agacgatgtt
     1081 cgccgcgtga aagaccggct cgatatagtg gaggtcatcg gcgattacgt ccgtctgaag
     1141 aaggcgggga acaactttct cggcctctgc cccttccacg acgagaagac gccctcgttc
     1201 aacgtctccc cgtcgcgcca gacgttccac tgtttcggct gcggtcaggg aggggacgtc
     1261 ttctccttca tcatggaaaa agaaaacctc tcctttcggg aggcgctcga aaccctcgcc
     1321 gaaagggccg gagtgcagct ctcctccgag ggcgcgcgta cgctcaggga gcgcagcctc
     1381 ttcgacatca tggagagggc ctgttcccac ttccgctcct tgcttgaaaa gcccgaaggg
     1441 gagcacgccg cggcgtacct cgcccggcga aatctcccac gcgaggcgtg gcggaacttc
     1501 gagctcggct gggcgtcccc ctcctgggac gctctctgga aggcgctctc caacgaaggc
     1561 gtctcctttc gcgaggcttt cgacgcgggg ctcgtactgg aggggaaaaa cgggccgtac
     1621 gaccgcttcc gcggacgaat catcttcccc gtcagggaca tttccggacg gctcctcgct
     1681 ttcggaggac ggctgctcga cggcgaaggg gcgaagtaca tcaacagccc tgaaggtgtg
     1741 ctctacagca agcggcgttc gctctacctg ctcaacaggg cgaaggaggc ggcgcgctcg
     1801 aaggacaggc tcatcctcgt ggaggggtac atggacgcca tacgcctcca cctgaacggt
     1861 ttcgaggaga ctgcggcctc tctgggcacc tccctcacgg aggaacaggc gctgctgatc
     1921 cggcgcctca ccgaccgctg cttcatctgc tacgactcgg atatcgcggg gcaggaggcg
     1981 tccatccgcg gaatgtacgt gctgcagaag gcgggactgg acgtccgcgt cgtcgaaatt
     2041 cccgagggaa aggatccgga cgatctgctc tcgcttccgg atggcaaggc gctcttctcc
     2101 aaagctctcg aagacgcccg tccgctggtg ctccaccaca tccacctccg gagggagatg
     2161 ctccgtactc ccgagaagcg ccgaaaagcg tcggaggaca ttatctccgg aatcgcgatg
     2221 ctctcccctg tggacgcgtc gccgtatctt ccggctgtcg aagcggcgct cggcgtgctg
     2281 catcatgaat tcatcccgat gctggaggag cagcgaagga agcactcttc gcaggcacgg
     2341 gagcctgtcc gcgacgtccc tccggacgaa gagaccggcg aagaacaaca gcctcccgac
     2401 cttgtggagg ctgcgttgtg cgcgctcctt tggagggact gcgaccttcg ccgatcggcc
     2461 gaccctgtgg aaattgtgtc gatgctctcc gacgcgcggc tgcagacgct tgcgtccgcg
     2521 ctgctctcgg cgtcttccgc tgaggacatg gagatctact ggcacgacat cggcgatacg
     2581 ttcccgatga aggcgatcgc cgccggagga gtctactgcg acgaacttga gcgggctcac
     2641 gatccctaca cgattctggt ggacattctc tccgtacgga aacacaaacg ggagtacgat
     2701 ctgctcaaga cgaagctctc caggggaaca gccgcaaacg atgaattgat gcgcttccag
     2761 aagctggcca tgattttaaa gagcgggaaa ggaaaaggga ctttgtgaaa gaattgaaat
     2821 tcagggggct tttttgatat acttccctgt ggtcttgtgt ctggaggaac gaagcgatgc
     2881 gattggataa atttttgaag cttgcaaggc tcgtcaaacg gagaacagtc gctcaggaga
     2941 tgatcgaagt cggggcggtt cgaatcaacg gaagacagtg caagccttcg gcggaagtcc
     3001 gtgtgggcga ttccgttgag atagcgtacc cctcgcgttt tctctcgctg gaggtcctgt
     3061 gcgccgacga ggcggcgttg aagcgaatgc cgcctgaaga ggcatggtct ctgcgggagg
     3121 agcggcgtgt ggatccatcg gaaagaccgt ggtgatactg cgcccggcaa gctgaacagt
     3181 tcctccccag cgggccgggt gtgtggcgtt ttggaatcgt ccggtgcgtc aaaagcatct
     3241 tcgggcgaag agcgtacttg aaaaggagtt gaggtgtatg agcagtatta tcggagtgca
     3301 cggccgggag atactcgatt ctcgcggcaa tccaacggtt gaagttgaag tgtggcttga
     3361 cagcggcgct tccggaagag cggctgttcc gtcaggggcg tccacaggaa cgtacgaggc
     3421 ggtggagctt cgcgacggcg gtccccgcta tctcggcaag ggagtactga acgcggtcaa
     3481 caacgtcaat gaaaaaatag cgcccgaact gatggggttc gacgccgacg accaggcgga
     3541 ggtcgacgcg gcgctcatcg aacttgacgg cacgcccaac aagggcgacc tgggcgccaa
     3601 cgccgttctc ggcgtgtcgc tggcggtagc ccgcgcggcg gcggacgacc acgatcttcc
     3661 cctgtggtcg tggatcggag gcctcgggcc cttctcgctg ccgacgccga tgatgaacgt
     3721 ggtcaacggc ggagcgcatg cggacaacaa cgtcgatatt caggagttca tgctggttcc
     3781 ccacggcgct gagaccttcc ccgaggcgct gcgtatgggc gtcgagacgt accataccct
     3841 gaagaaggcc atccacgccc gcgggtacag cacggccatc ggcgacgagg gcggcttcgc
     3901 ccccgacctg aagagcaacc gcgaggccat cgacctgatt cttgaggccg tggagaaggc
     3961 gggatacgcg ccgggcaagg acatctccat cgcgctcgac cccgccgcgt cggagttctt
     4021 caaggacggg aagtatcatt tcgcgggaga gaaaaagtcc ttcactcccg aggagatggt
     4081 ggactactac gagcagctct gcaaggacta cccgattctc tccatcgagg acggcatggc
     4141 ggaggacgac tgggccggat ggtcggtcat gacgaagcgc ctcggctcca agatccagat
     4201 cgtcggcgac gacctcttcg tcaccaaccc ggagaagctg tcgagaggta ttgaggaggg
     4261 agtggccaac gccgttctca tcaagctcaa ccagatcggc tcgctctccg agacgctcaa
     4321 cgtcatcgcg ctcgcgaagc gctacggata cgcgaccgtc atctcgcacc gttccggcga
     4381 gaccgacgac tccttcatca gcgacctcgc cgtggccacc ggagccgggc agatcaagac
     4441 gggagccccc gcgcgcgtcg acagggtcgc gaagtacaac cagcttcttc gtatcgccga
     4501 ggagctggac cgggcgccgt acgcgcgtct ctcgcagttc aggggtaatc cgaacaggta
     4561 ggacacaaaa gacattaagg aggcgttcct tttgaaaggt attgtatcca caaccaacgc
     4621 gcccgcagcg gtcgggccgt acagccaggc agtcaaggcg gggcagttcc tcttctgctc
     4681 cgggcagatt cccatcgatc cggcgacggg gcagttcgtt ccgggcggcg tcgctgagca
     4741 ggcaaaacag gttctgaaca acgtaaaagc cgttcttgaa gctgccgggt attcccttgc
     4801 cgatgtgatc aaggcgaccg tcttcgcgac cagcatgaac gactttgctg cggtgaacgg
     4861 cgtctacgcg acatatttcg agaaagagcc ccccgctcgt tccttcgtcg aagtgagcgc
     4921 gctcccaaaa ggcgcccttg tggaaatcga agtcgtcgcc tggaaggaat gattccggcg
     4981 gccggaggcg ccgcctccgg ccctctactc ttgaaggatg tgcgcacatg aaggatacga
     5041 agatagcgga gcccacagtc gagcggcttg tccagtatca ccgtctttta gagcagctcg
     5101 cggccgaggg gcagaaagtg gtctcctcca gggagatggg ggagatgctc tcgttcaagg
     5161 ccagccaggt gcggaaggac ctctcgtact tcggcgagat aggcaagcgg ggcgtggggt
     5221 atcatgttga tcgtttattc aagcatatcg gcggaatcct tgcgtccccc aggctctggt
     5281 ccatcggtct ggccggcgta ggaaaactcg gcgaggcgct tttagggtat caggcgttcc
     5341 ggaacgagaa gtttcagatt aaggccctct tcgacgtcga cccttcgaaa atcggaacgc
     5401 atatccaggg aactccctgc tactccatag aggacgctcc ccgcgtgctg cgagaggaag
     5461 gtatcgatgt cgtcatcatc accgtgccga cgggagcggc ccagtccgtt gcagacaggg
     5521 tcgtttctgc aggaacggtg aaggggatac tcaacttctc cccggtcacg ctctccgttc
     5581 cggacagcgt gctgctctac agcgtggata tttcgataga actggagaaa cttctttttt
     5641 acctgaagca tcgcgaacgt tagcagtttt ttttgataga atatcggggt tgttttccgt
     5701 cttgccgggc agggcgaggc aaccttgcac cctaatatca aggaggcgtt ttttttggaa
     5761 cagcagtgtg gacaggcggc aggcggcgca ggaggacagg gaagtctcat gggaatgctc
     5821 ttccctctcg cgatattcgt ggtcattttc tacttcttca tcatccgtcc ccagaagaag
     5881 cggcagcgtg cccaggacga acttctctca tcgctttcga ggggggacca ggtggtcacc
     5941 atcggcggtt tcttcggaac catccgtgaa gttcgcgacg acagccttgt cgtcgaaata
     6001 gccgacggcg tgaaggtacg ccttctcaag tcggcggtgg cttcgaagcg cgctcccgcc
     6061 acccaggctc ccaaggagga gcccgcacgc gagaaggaag aggagaagaa ctgaccgtat
     6121 ctgccggttc gatgtgaagt gcgccccgcc cccttccgat gcaaccgcag gtgcggcggg
     6181 gaaacggggg gaggtcgccg cgaggcgatc cccccgctcg cgtcccgtcg gccatactgt
     6241 tggcgggagt tttttcgcgg gggaatgaac aaccaatttt gcgaacacaa cggagggaat
     6301 tcttatgttg agacgagacc gctggcggct tggaatagtc gccatcgtgg tgcttgcggc
     6361 cctgttctcg gtctttccca ttcagggaag gatcaatctg gggctagatt tgaagggcgg
     6421 cgcccacatc gttcttcagg cgaaggacac cgcggacagc aaggtgacgg aggacagcat
     6481 cgaacgtctt cttgctgtgc ttcggaatcg cgtggaccag tacggtgtgg cggaacccgt
     6541 catccagagg gagggtaccg acaggatcat cgtcgacctc cccggtatcc aggatccgga
     6601 cgcggcattg gagcttatcg gcaagacggc gcttcttgag ttccgccagg ttgtgacggc
     6661 gacgtcgcct attcctccct ccgcagagcg gaagaactac gacagcgacg ccgagttcga
     6721 gcggg
//
LOCUS       DAOTIL010000032         6723 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminobacteriaceae bacterium isolate AMR_MDS_3026
            NODE_13505_length_6723_cov_3.965057, whole genome shotgun sequence.
ACCESSION   DAOTIL010000032 DAOTIL010000000
VERSION     DAOTIL010000032.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868050
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminobacteriaceae bacterium (wastewater metagenome)
  ORGANISM  Aminobacteriaceae bacterium
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae.
REFERENCE   1  (bases 1 to 6723)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6723)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:19:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,224
            CDSs (total)                      :: 3,198
            Genes (coding)                    :: 3,184
            CDSs (with protein)               :: 3,184
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6723
                     /organism="Aminobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13505_length_6723_cov_3.965057"
                     /isolate="AMR_MDS_3026"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:3034609"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..392
                     /locus_tag="P5201_01235"
     CDS             <1..392
                     /locus_tag="P5201_01235"
                     /inference="COORDINATES: protein motif:HMM:NF012515.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="PfkB family carbohydrate kinase"
                     /protein_id="HRV97186.1"
                     /translation="DFLAPNETELEIITGMPVASTGDVLAAARSLVNEGVPKVLVTLG
                     AKGSLLVDSKEHLFVDPFPVKPVDTTGAGDAFIGAFIVEYLGSKDIKGAMKMANRYAA
                     LSTTREGTQKSFLHRAEFDTLLEERKA"
     gene            389..1039
                     /gene="deoC"
                     /locus_tag="P5201_01240"
     CDS             389..1039
                     /gene="deoC"
                     /locus_tag="P5201_01240"
                     /EC_number="4.1.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008520200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004139 - deoxyribose-phosphate aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0009264 - deoxyribonucleotide catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribose-phosphate aldolase"
                     /protein_id="HRV97187.1"
                     /translation="MKSREIAKYIDHTLLKPEASEEAVRKLCAEARDFGFASVCVNGG
                     FTELVARELKGSDVKTCVVIGFPLGAGTSAAKAFEAADAVSRGAEEIDMVISVGRLRS
                     GETASVREDIRAVVQAAEGALVKVIIETCLLTDEQKELACRLAEEAGAHFVKTSTGFS
                     TGGATAEDVALMRRVVGDRLGVKASGGIRTREDAERMIAAGASRIGASASVAICAG"
     gene            1129..2184
                     /locus_tag="P5201_01245"
     CDS             1129..2184
                     /locus_tag="P5201_01245"
                     /inference="COORDINATES: protein motif:HMM:NF019850.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Mur ligase family protein"
                     /protein_id="HRV97188.1"
                     /translation="MNSIHSIPIRILVTGSRGKSSLVRLLSAALVSFGLNVRGRITGV
                     LPRELLPGDGILSPLKETLLLRSGPASVEEMRWWLSTLPRGTDAVVMENSAVAPELQA
                     LAFRWLQPCCTVLTNVRPDHEEVWGRGEENAASALCSGIGDPGGMTCPEHPVVLPRSV
                     AEKQCVQRLLSAQHCRLLPCGEGNAFKETHLALVQEVCRLFGFDERQSRAAAAALPPD
                     VADFAFFREGDGVLAAAFSANDTESTEQLFVSTGWSREETTLLFNARKDRVRRFRSFL
                     PWIRSFPWKKVCVTGGRPLFLSSPAVYSPLLDAPSLAGFIRSTGRVFGCGNVAGTPLE
                     YLESKFLQFRERQDGVS"
     gene            2181..2618
                     /locus_tag="P5201_01250"
     CDS             2181..2618
                     /locus_tag="P5201_01250"
                     /inference="COORDINATES: protein motif:HMM:NF025467.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="poly-gamma-glutamate biosynthesis protein
                     PgsC/CapC"
                     /protein_id="HRV97189.1"
                     /translation="MTEYSLLLVGLGICLGLFFFHRTGYSPGGIITPGFLALELGSPE
                     RVAAAFVIGGCVAALLSLVVRVTGAYGRQRTGIALLLALAFRLFAGGGTTLSYLWIGW
                     VVPGLIGADMQRQGAIPTIGAALSTAFASAMAARLLISAGALL"
     gene            2615..3679
                     /gene="pgsW"
                     /locus_tag="P5201_01255"
     CDS             2615..3679
                     /gene="pgsW"
                     /locus_tag="P5201_01255"
                     /inference="COORDINATES: protein motif:HMM:TIGR04332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="poly-gamma-glutamate system protein"
                     /protein_id="HRV97190.1"
                     /translation="MNGLSARSRAFARRARWGLLIPACVLAFLWFFQGVSFLSEEEEA
                     LWRKVRAAQTSLAAWRDASQLPALYPEYDFSKTDPWRSGFIGIEWSPVTTTLGPIEAK
                     RTAADPLWSVYALREFRKFGLKEGETIAMISSSSFPGLVYSLLAAAEHLGLRVLWIHS
                     LGASTWGANIPEMSWPKIGELLRNGGFLSRKPDWYTLGGGNESGGGMSPEGRKTLLAA
                     ALHDGVPMLEAATLQEMIELKTKRILSVKPRLVLAVGGSHSTFGKADIVPPGGGWYTP
                     EDTERVDMGEGVFRAVLLSGVPVLHFLNLRPLARSAGIPFDVPPRSRFGGGAGDAACA
                     AGLLFYVVFLFRFHRWRRDE"
     gene            3676..5214
                     /locus_tag="P5201_01260"
     CDS             3676..5214
                     /locus_tag="P5201_01260"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HRV97191.1"
                     /translation="MICSHDVKQEITRQEEHVFLDGLSQKEKDGEVQSALLPRGSAEK
                     FFFSGLFFLGLWLLAQGLSAQPVLEILGRLPETMDSGDLLSAASMLVLMNSLRAIALY
                     LGWFLLGSGLGALHPSVSFLSWFVPVVAIPVTYFILPSLGEGVQLHFGFPAVLSVSSV
                     LVLRYLTRGIADWGYKAIALALFVFSFQWLDVIPALTSWGAGWGELSSAVKTAALLME
                     REYLLNRAGIAVFGGLFCAGVITTELLVSYSARLYSLALLRDRENKVALLREENLRNR
                     SLVEMQQLVHDLKRPLTTIMGLADVIASGKSAPSAGKYGAVIVEAGRSMEEMISEILH
                     EDFRRPIALGDLLQYVFTQISPFDWRETVSLSAEEGILDEYVTVNVVRLSRALVNLLE
                     NAHRANTLVNGSRIVMRISGTQEFAEIAVLDEGPGFRRTRGEEDGKPLKAGGLSGMEW
                     SSSSGWNSTGLGLQYVTMVVRNLGGKFVIEDLPDGGARTAIVLEKNAAGKESSKAGRE
                     AFGM"
     gene            5244..5888
                     /locus_tag="P5201_01265"
     CDS             5244..5888
                     /locus_tag="P5201_01265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014163897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HRV97192.1"
                     /translation="MPLHIGAIDDDRSILYTLEAMASTEGWRMLTTNVPEECLDWIKL
                     DEVDILLVDYHMPVMNGLHLIRKARELSSKLVLIALTVEDGGDLAGQLVLAGADDFIT
                     KPIRLADFTARIRLHEKLFAHREQLNWEERKKGVSKDTMRKVLEKLKEFSSPKDCDDV
                     AAACGLAYVTAHRYLEYLAERGLIIRSAGVQDGRPGRPKTYYAVPVLSSNAASQ"
     gene            complement(5951..6694)
                     /locus_tag="P5201_01270"
     CDS             complement(5951..6694)
                     /locus_tag="P5201_01270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013047638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HRV97193.1"
                     /translation="MKKILFALFALLVLGTAGWGNVMEKDALLVGTESTYPPYEFRDA
                     NNQLKGFDIEMMETIAAKIGKKIEWVDMPFDSLIPSLLAKKIDIVAAGMSATEERAKR
                     VAFSTNYEISISAFIVAEGNDSMKVLEDMKGKTIAVQLGTVQETYSQSLEGVVVKSFQ
                     KFDDCVREVILGRVDATLMDTPVAKSYVEHKDFQGKIKIAFEQEITGSGKALAMNLGE
                     TELIEAVNKALAELEGSGELDALKKQWFK"
BASE COUNT         1280 a         1855 c         2031 g         1557 t
ORIGIN      
        1 tcgatttcct tgcgccgaac gaaacagagc tcgagattat taccgggatg cctgtcgcat
       61 cgaccggaga tgtgctggct gccgcacgga gccttgtgaa cgagggcgtt ccgaaggtgc
      121 tcgtaacgct tggcgcgaag ggatcgcttt tggttgattc gaaggagcat ctcttcgtcg
      181 accccttccc tgtaaagcct gttgatacca caggagcggg agacgcattc atcggcgctt
      241 tcatcgtaga gtatcttggc tcgaaggata tcaaaggtgc gatgaagatg gccaatcgtt
      301 acgcagcgct ctcgacgacg cgcgaaggaa cgcagaaatc cttccttcac cgagccgagt
      361 tcgatacgtt gcttgaggag agaaaggcat gaaatcccgc gaaattgcca agtacatcga
      421 tcacaccctc ttgaagccgg aagcctcgga agaggcggtt cgcaaactct gcgccgaggc
      481 gcgggatttc ggctttgcct cggtctgcgt caacgggggg ttcaccgaac tggtcgcccg
      541 cgagctgaag ggaagcgacg tgaagacctg cgttgtgatc ggattcccgc tgggagcagg
      601 aacatcggca gcgaaagctt ttgaagcggc ggacgcggtc tcccgcggag ccgaggagat
      661 cgatatggtg atctcagtag gacgactgcg ttccggggaa acggcctccg tacgtgagga
      721 catccgcgcc gtggtacagg ccgcggaagg cgcgctcgtc aaggtcatta tcgaaacctg
      781 cctgctcaca gacgagcaga aggagcttgc ctgccgcttg gcggaggagg cgggagcgca
      841 cttcgtgaag acctcgaccg gcttttccac gggcggagcg acggccgagg atgttgccct
      901 gatgcggcgc gtcgtgggcg acaggcttgg agtaaaggct tccggaggca tccggacgcg
      961 agaggacgct gaaaggatga tcgcggcggg ggcgtctcgg atcggcgctt ccgcttccgt
     1021 cgccatctgc gcaggctaat acggtccccg tgaggtcccc gtctccattc ggcgggagcg
     1081 gggactttgc gttccgcttc tttcggcgcg aggtgtacaa tatacttcat gaacagcatt
     1141 cattcgattc ccatccgaat actcgttacc gggagccggg gaaaaagctc cctcgtccgc
     1201 cttctttcgg ctgctttggt ctccttcggt ctcaacgtcc gcggacggat cacaggggtt
     1261 ctcccaagag aacttctgcc cggcgacggg atcttatctc ctctgaagga gacgcttctg
     1321 cttcgttccg gcccggcgag cgtcgaggag atgcgctggt ggctttcaac ccttcccagg
     1381 ggaacggacg ccgtcgtgat ggagaacagc gcggtcgccc cggaattgca ggcccttgcc
     1441 ttccggtggc ttcaaccgtg ctgcaccgtg ctcacaaatg ttcgtcccga ccacgaggag
     1501 gtttggggaa gaggcgaaga gaacgccgcg tctgcgctct gttcaggaat aggcgatccc
     1561 gggggtatga cctgtccgga gcatcctgtc gttcttcccc gttctgtcgc cgaaaaacag
     1621 tgtgtgcagc gcctgctctc agcacaacat tgccgccttc ttccctgcgg cgaaggaaac
     1681 gctttcaaag aaacgcacct cgcccttgtc caagaggtct gccgtctctt cggcttcgac
     1741 gagcggcaaa gccgtgccgc cgccgccgcg ctgcctccgg atgtggctga tttcgccttt
     1801 ttccgcgagg gagacggggt gcttgcggca gctttttctg caaacgacac cgaatcaact
     1861 gagcagcttt ttgtatccac cggatggagc agagaggaga ccactcttct tttcaatgca
     1921 agaaaggata gggtgaggcg tttcaggtca tttcttccat ggattcgttc cttcccgtgg
     1981 aagaaagtct gcgttaccgg cggccgacct ctctttttat catcccctgc cgtgtattcg
     2041 ccgctccttg acgccccgtc ccttgcgggt tttatccgtt caacgggcag ggtcttcggg
     2101 tgcggcaacg tggcggggac tccccttgag tatctggagt cgaaatttct gcaatttcgt
     2161 gaaaggcagg atggcgtatc gtgacggaat acagtctgct tctggtcgga ctggggatct
     2221 gcctgggctt gttctttttc caccgcacag ggtactcccc ggggggcatc attacgcccg
     2281 gttttctcgc ccttgaactc ggctcgccgg aaagagtcgc tgcagcattt gtcatcggag
     2341 gatgtgttgc ggccctcctc tcgctcgtcg tacgtgtaac cggagcgtat ggccgtcagc
     2401 gaacggggat cgccctgctg cttgcgcttg ccttcagatt gttcgccggg ggggggacga
     2461 cgctctcgta tctgtggata ggatgggtcg ttcccgggct gataggcgcc gatatgcaac
     2521 ggcagggagc gattccgacc atcggcgctg cgctttccac ggcgttcgcc tccgcgatgg
     2581 ctgcccgttt gctgatttcc gccggagcgc tcttatgaac ggattgagcg cgcggtcccg
     2641 cgcctttgct cgtcgggcac gatggggatt actcataccg gcgtgcgtgc tcgcctttct
     2701 ctggtttttt caaggcgttt cgtttctttc ggaggaggaa gaggctttgt ggcggaaggt
     2761 gcgcgccgcc cagacgagcc ttgctgcgtg gcgcgatgcg tcgcagctcc ctgccctgta
     2821 cccggaatat gacttcagca aaacagatcc gtggagaagc ggtttcatcg gtatcgaatg
     2881 gagcccggtg acgacgactc ttggaccgat agaagcgaag cggacagctg cggatcctct
     2941 ctggagcgtc tacgctttgc gggaattccg gaagtttggg ctcaaagaag gagagactat
     3001 cgccatgatc tcttcgtcct ctttccccgg cctggtgtat tcgcttcttg ccgctgccga
     3061 gcatcttggt cttcgcgtcc tatggatcca ctcgcttggc gcctccactt ggggcgcgaa
     3121 cattccggaa atgtcctggc cgaagatagg agaactcctt agaaatggag gcttcctctc
     3181 acgaaagcct gattggtata ctttgggcgg agggaacgaa tcgggcggcg gtatgtcgcc
     3241 ggaaggaaga aagacgcttc ttgccgcagc gctgcacgac ggtgttccga tgctcgaagc
     3301 cgcaacgctt caggagatga tcgagctgaa aaccaagcgc attctttccg tcaaaccgcg
     3361 gcttgtactt gcagtcggag ggtcgcacag tacctttggg aaagctgata ttgtccctcc
     3421 cggcggaggg tggtacacgc ctgaagacac ggaaagagtc gacatgggcg aaggggtgtt
     3481 ccgtgctgtt cttctttcgg gcgtaccggt gcttcacttt ttaaacctcc ggccgcttgc
     3541 acgaagcgca gggattcctt tcgacgtccc tccccgctct cgtttcggcg gcggggccgg
     3601 agatgctgcg tgcgccgcag ggctcctgtt ctacgttgtt tttcttttcc gctttcaccg
     3661 ttggaggaga gacgaatgat ctgttcccat gacgtgaagc aggaaatcac ccggcaggaa
     3721 gagcatgtgt tcttggacgg tctctctcag aaagagaaag atggagaagt ccaaagcgct
     3781 cttcttcctc gcggaagcgc cgaaaaattt ttcttttccg gtcttttttt tctggggttg
     3841 tggctgctgg cgcaggggct ttccgcccaa cctgtgcttg agattctcgg caggcttccc
     3901 gagacgatgg acagcggcga tcttctctcg gccgcgtcca tgctcgtcct gatgaactcg
     3961 ctcagggcca tcgcgctcta tctcggctgg ttccttctgg gaagcggcct gggagcgctt
     4021 cacccctcgg tctcttttct ctcgtggttc gtgcctgttg ttgccattcc ggtgacatat
     4081 tttattcttc cgtcgttggg ggagggggtt cagctccatt ttggcttccc tgccgtgctg
     4141 agcgtgagca gcgtgcttgt cctccgctat ctgaccagag gaatcgccga ctgggggtac
     4201 aaagccatcg cgctcgccct tttcgttttc tcttttcagt ggctcgacgt cattccggcg
     4261 ctcacatcct ggggggcggg ttggggcgag ctctcctccg ccgtgaagac cgcagcgctt
     4321 ctgatggaac gggagtatct gctgaacaga gcgggaattg cagtcttcgg aggtcttttc
     4381 tgcgccggag tcatcacaac ggaactcctc gtcagctaca gcgcccgtct ctacagcttg
     4441 gctcttctgc gggaccggga gaacaaagtg gctcttttgc gggaggagaa cctccggaac
     4501 cggagccttg ttgaaatgca gcagcttgtt cacgatctga agcgccccct gacgacgatc
     4561 atggggctgg ccgacgttat cgcctcggga aaaagcgccc cgagcgcggg gaaatacggc
     4621 gccgtcattg tcgaggcggg acgttcgatg gaggagatga tctccgagat tctccacgag
     4681 gattttcgcc gtcctattgc cctcggggat cttctgcagt acgtcttcac ccagatcagc
     4741 ccgttcgatt ggcgagagac cgtgtcgctc tcagctgaag agggaatatt ggatgaatac
     4801 gtgacggtga atgtggtgcg tctttccaga gcgttggtga atctgctgga gaatgcgcac
     4861 agagcgaata cactcgtaaa cgggagcagg attgtgatgc gcatctccgg aacgcaagag
     4921 tttgctgaga tagctgtcct cgacgaaggt cccggttttc gaaggacgcg gggagaagaa
     4981 gacgggaagc ctttgaaggc cggaggtttg tccgggatgg agtggagttc atcttcagga
     5041 tggaattcca ctggtcttgg tctccagtac gttacaatgg tcgttaggaa tctcggaggc
     5101 aagtttgtca tagaggatct ccccgacgga ggggcgagaa cggcgatagt ccttgaaaaa
     5161 aacgcagccg gcaaagagtc ttcgaaggcg gggcgtgaag ccttcggaat gtaaaagatc
     5221 ctaatgaaag gatgggaagc gcaatgcctc ttcacatcgg tgcgatcgac gacgacaggt
     5281 ctattctcta tacgctcgaa gccatggcct ccacggaagg gtggcgcatg ctcacaacga
     5341 acgttccgga ggagtgcctc gactggatca aactcgacga ggtggatatt ctgctcgtcg
     5401 actatcatat gcctgtaatg aacggtctgc acctgatacg aaaggcgagg gagctctcct
     5461 cgaagctggt gctgatagcg ttgaccgtgg aggacggcgg cgacctcgcc ggtcagctcg
     5521 tgctggccgg agcggacgat tttatcacga agccgattcg gctggcggat tttaccgccc
     5581 ggatcaggtt gcacgagaag ctctttgctc accgggagca gctgaactgg gaagaacgga
     5641 aaaaaggcgt cagcaaggat acgatgcgga aggtcctcga gaagctgaag gaattcagct
     5701 ctcccaagga ctgcgacgat gttgcggccg cttgcgggct ggcgtatgta acggcgcaca
     5761 ggtatctgga atatcttgcc gaacgcgggt tgatcattcg cagcgccggg gtgcaggacg
     5821 gccgtccggg acgtcccaag acatactacg cagttcccgt cttatcttcg aacgcggcct
     5881 ctcaataaaa aaagcggggc tctccgtgcg tggagagccc cgcttcattc agtacgaaaa
     5941 aaactgaagt ctacttaaac cactgctttt tcagagcgtc cagctcgccg cttccctcaa
     6001 gttcggcaag ggccttgttc acagcttcga tgagttccgt ttcgccgaga ttcatggcaa
     6061 gagctttccc tgagccggtg atttcctgct cgaaggcgat cttgatcttt ccctggaagt
     6121 ccttgtgctc gacgtagctc ttcgcgacgg gagtatccat caatgttgcg tcgacgcgtc
     6181 ccagaatgac ttcgcgaacg caatcgtcga acttctggaa ggattttaca acaacgcctt
     6241 caagagactg actgtaggtc tcctgtaccg tcccaagctg gacggcgatc gtctttccct
     6301 tcatgtcttc gaggactttc atcgaatcgt tgccctcggc cacgatgaac gcgctgatcg
     6361 agatctcgta gttcgtggag aacgcgaccc ttttcgcccg ctcctcggtg gcgctcattc
     6421 cggcggcgac gatgtcgatc ttcttcgcga gaagcgaagg aatgaggctg tcgaacggca
     6481 tatcgaccca ctcgattttc ttccctattt ttgcggcgat cgtctccatc atttcgatat
     6541 cgaagccttt gagctgattg tttgcatcac ggaactcata aggggggtat gtgctctccg
     6601 tgccgacgag caaagcgtcc ttttccatga cattgcccca tccggcggta ccaagaacca
     6661 gcagcgcaaa cagtgcgaac agaattttct tcatcgtttt ctctcctcca tagttgcctt
     6721 tgg
//
LOCUS       DAOTIL010000033         6638 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminobacteriaceae bacterium isolate AMR_MDS_3026
            NODE_13705_length_6638_cov_4.524077, whole genome shotgun sequence.
ACCESSION   DAOTIL010000033 DAOTIL010000000
VERSION     DAOTIL010000033.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868050
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminobacteriaceae bacterium (wastewater metagenome)
  ORGANISM  Aminobacteriaceae bacterium
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae.
REFERENCE   1  (bases 1 to 6638)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6638)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:19:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,224
            CDSs (total)                      :: 3,198
            Genes (coding)                    :: 3,184
            CDSs (with protein)               :: 3,184
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6638
                     /organism="Aminobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13705_length_6638_cov_4.524077"
                     /isolate="AMR_MDS_3026"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:3034609"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..537)
                     /locus_tag="P5201_01275"
     CDS             complement(<1..537)
                     /locus_tag="P5201_01275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005662357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase HflX"
                     /protein_id="HRV97194.1"
                     /translation="MRKAILAAIELPKQEFAPTVLLDELTLLLENLGIQTAGTVIQRR
                     ERPDSAFLLGKGKVDEIALFCKAAGADLVVCNESLTPGQKSNLQHTIGLEVWDRPFVI
                     MKIFEKRARTSEAKLQVEMALCRYEIPHLKGLGAQMSRLGGGIGTRGPGETEFERHRR
                     KLERRVRDIGRKLEAMKHK"
     gene            complement(534..1190)
                     /locus_tag="P5201_01280"
     CDS             complement(534..1190)
                     /locus_tag="P5201_01280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005662359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HRV97195.1"
                     /translation="MMTYWNRVFYFFVKWFFFIALKIYNRLSIRWTSSLPQRNVIVVA
                     NHASNLDPVIMGAVFPRRLRYLAKEELFKPFIFGNMVRALGSIPVRKSDSQSAGTALR
                     TFLKLLESGENVLIFPEGSRSLNGELQPLEGGVALIALKSGAPIIPSFISGTFEAMPP
                     GSRWVKPLHLSIVFGDTIDSAKYIPFGKEGREMLLKDLNEVFISLSTADLPGKSRNNS
                     "
     gene            complement(1195..1863)
                     /gene="cmk"
                     /locus_tag="P5201_01285"
     CDS             complement(1195..1863)
                     /gene="cmk"
                     /locus_tag="P5201_01285"
                     /EC_number="2.7.4.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008523020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004127 - cytidylate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(d)CMP kinase"
                     /protein_id="HRV97196.1"
                     /translation="MNCVSFVAAIDGPAGAGKSTLARKVANLLRMRYLDTGALYRALA
                     FSLYSSQVSPEEGPEMEAAIAGISVEILENGLLLNGKEVGKEIRSPLVDSIVSSYAAL
                     PSVRKRLLSIQREQGKHGSLVADGRDMGTVVFPDADVKIFLTASDEVRAKRRLLELRE
                     RGEDVTYEEVLQTIRERDRVDSERSTAPLRKAEGAIEVNTDALSIEEAVATLASIISK
                     RKGA"
     gene            complement(1860..2750)
                     /locus_tag="P5201_01290"
     CDS             complement(1860..2750)
                     /locus_tag="P5201_01290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006300837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HDOD domain-containing protein"
                     /protein_id="HRV97197.1"
                     /translation="MEGIDERSRELIKTRILGKIKEIRSFPQFVVETLRKLNDPDSSA
                     HDVAASLSRDEGLVIRTLKLANSAAYGISRDISSVAEAIALLGYKNISSIVLAASVYS
                     VMDKPLAGYALDRGMLWRHSLTVAYSSRYIAQLTKKGLPEEAYVGGLLHDIGKVVLND
                     YVRFGYGIIVKMVEEQQIPFLEAEIQVLGFDHAMVGAMLVEKWGLPEPYKYAVAHHHS
                     PNNLAEPKHQNLVDVVHLANSLCLMLGVGIGADGLQNYLYPEVLERLGISDYEMLMSD
                     LVDFVAQAEAELSEMEEAFQ"
     gene            complement(2772..3500)
                     /locus_tag="P5201_01295"
     CDS             complement(2772..3500)
                     /locus_tag="P5201_01295"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009201075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine synthase"
                     /protein_id="HRV97198.1"
                     /translation="MENGTRLNRFLAMCGIGARRKVEDVIASGRVRINGKVVLLPGYR
                     VENDMDVSVDGQTVQPQEHRYLVMNKPDGVVCAVEDGYDPTVLGLLPPEFQTVRLFPV
                     GRLDRESQGLLILTNDGAFAQEILHPSKRILREYEAMLDHAITRKEVRKWMAGSLLDG
                     RLIKPVNVLLLDKEPAGCWVSVVLAEGVKREVRRMAAEIGCDVVVLVRKRIGKMELKN
                     LQQGQFIEMSRGSLWNAIRSGGVV"
     gene            complement(3550..4158)
                     /gene="scpB"
                     /locus_tag="P5201_01300"
     CDS             complement(3550..4158)
                     /gene="scpB"
                     /locus_tag="P5201_01300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012869525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SMC-Scp complex subunit ScpB"
                     /protein_id="HRV97199.1"
                     /translation="MTSSLPRFETLSSTAKIIEAILFAAPSPVSVNEMATLLSLSPSA
                     VKSELEDLRSYLLRHEHGITVIESAGGWRMATAEDVSEIVGAFRETAIAQKIRLSKAA
                     VETLAVIAYNQPVTRSEIEELRGVRCERVIETLLSHALIRIAGRRKATGSPLLYRTTD
                     RFLETFGVLSISELPTLEELDELAPVDEPHFVPVDEEERTNG"
     gene            complement(4133..4906)
                     /locus_tag="P5201_01305"
     CDS             complement(4133..4906)
                     /locus_tag="P5201_01305"
                     /inference="COORDINATES: protein motif:HMM:NF014654.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="segregation/condensation protein A"
                     /protein_id="HRV97200.1"
                     /translation="MEERLGELAEFQAEIDGFSGPFDLLCWLVESRELEAASISVGQV
                     VRIYGAYLANTNKVSVSVVSEFLFMAASLVLNKIRALLPGRESLYESEEPENDPGEDV
                     LEKLSRYRPYRSAARDLAILKEERDRFFFRSAPEEEDDEPSFDLGDLFALCRLWWSLR
                     DLKRRTKNAAPYIFENEDDWSGVPSSLPEEEQIERKISEILSELNSDGSLRLSFLLRE
                     NRAMANFVVTLLALLEMSRMGRIRILQEELFDDIVITPL"
     gene            complement(4909..5904)
                     /gene="trpS"
                     /locus_tag="P5201_01310"
     CDS             complement(4909..5904)
                     /gene="trpS"
                     /locus_tag="P5201_01310"
                     /EC_number="6.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013048480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004830 - tryptophan-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006436 - tryptophanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan--tRNA ligase"
                     /protein_id="HRV97201.1"
                     /translation="MAQRVFSGMRPTGKLHLGHLAGALTNWVKLQENYKCFYSIVDWH
                     ALMSEYADSSMIAENCKEVLLDWLASGLDPEKCVIFVQSHIPQHAELHLALSMVTPLG
                     WLQRCPTYKEQILNIQNKDLSNYAFLGYPVLMAADILLYKAEIVPVGEDQSAHLEITR
                     EMARRFNSFFGNILPEPQIMLTPTPKVPGTDGRKMSKSYGNAINIADTPSEMWNKIRP
                     MITDPARERRTDPGDPEKCPVWDIHKVFNNNEDEKNEIASGCRSAGIGCVDCKKKLQA
                     HVERVMGPIQERRTFYEKSPDLLSDILREGAEKAGEVARRTIDEVINAIGFFKRG"
     gene            complement(5996..>6638)
                     /locus_tag="P5201_01315"
     CDS             complement(5996..>6638)
                     /locus_tag="P5201_01315"
                     /inference="COORDINATES: protein motif:HMM:NF013909.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="Nif3-like dinuclear metal center hexameric
                     protein"
                     /protein_id="HRV97202.1"
                     /translation="IDAMKAALDHSCSVLITHHPLIFHPLKRIDCAEGPGAQIAFAIR
                     HGLTVFSLHTNWDVAPGGVNAAIAEVLSLQEVRPLLPSTTGAWGSGAAGKFPRPLNFL
                     ECGAAIRDALHLSRLELHGDMKTSVSTLALCGGSGGTFLPEAFACGAEAYFTADMKYH
                     ERLDALDKGVSLFIADHGEMERFSLTALAETVSKATGEKAKILDVSQPAFCMI"
BASE COUNT         1584 a         1834 c         1635 g         1585 t
ORIGIN      
        1 cttgtgtttc atcgcctcca gttttctgcc gatatcccgg actcttcgtt caagtttccg
       61 acgatgtctt tcaaattcgg tttcacccgg accgcgtgtt ccgatacctc ctccaaggcg
      121 cgacatctgc gctcccaacc ctttgagatg aggaatttcg tatctgcaca acgccatctc
      181 gacctgcagc tttgcttcgg acgtcctcgc gcgcttttcg aatatcttca tgatgacaaa
      241 aggacggtcc cagacttcca ggccaattgt atgctgcaga ttactcttct gtcccggggt
      301 gaggctttcg ttacaaacaa cgaggtcagc ccccgcagct ttacaaaaaa gagcgatttc
      361 gtcgactttc cccttgccaa gaagaaacgc tgaatccggt cgttcgcggc gctgtattac
      421 agtccctgcc gtttggattc caagattttc aagaagaagc gtcagttcgt cgaggaggac
      481 tgtcggagca aactcctgct ttggaagttc aatcgccgca aggattgctt ttctcatgaa
      541 ttgttcctgg atttgccagg cagatcagca gtgctcaaag agatgaaaac ctcgttaaga
      601 tccttgagca acatctctct tccttctttc ccgaaaggga tgtatttcgc cgaatcaatg
      661 gtatccccaa agacaatcga aagatgaaga ggttttaccc agcggcttcc ggggggcatt
      721 gcctcgaaag tgccggagat gaaagaggga atgatcggcg ctccggattt taacgcaatg
      781 agcgccaccc ctccttcgag aggctgaagt tccccgttta gagaacggct tccctccgga
      841 aagatcaata cgttctcccc gctttccagg agtttcagga aagtgcggag ggctgtccct
      901 gcgctctggc tgtcgctctt tcgtacgggt atggagccaa gcgccctgac catattgccg
      961 aatatgaacg gtttgaagag ctcttccttc gcaagatagc gaagacgccg cgggaataca
     1021 gcgcccatga ttacgggatc cagattgctc gcatgattgg cgacaacaat gacgttgcgt
     1081 tgagggagtg aagacgtcca tctgatggac aaacggttgt agatcttcaa ggcaatgaag
     1141 aaaaaccatt tgacaaaaaa atagaaaacg cggttccaat acgtcatcat ccggtcacgc
     1201 tccttttcgt tttgagatga tcgaagcgag cgtcgctacg gcttcttcga tagaaagagc
     1261 gtcagtattc acttcgatag ctccttccgc tttccgaagc ggcgcggttg aacgctcgct
     1321 gtcgactctg tcgcgttcgc gaattgtttg cagcacttcc tcataggtca catcttcgcc
     1381 tcgttcacgt agttcaagaa ggcgacgctt cgcacgcact tcgtcggaag ccgtgaggaa
     1441 aatcttgacg tcggcgtcag ggaagaccac agtacccata tcccggccgt cggcgacgag
     1501 agagccgtgt tttccctgtt cacgttgaat cgagaggagg cgtttccgaa ctgagggaag
     1561 ggcggcataa gaagagacga tggaatcaac aagaggagaa cgaatttctt tccccacttc
     1621 ttttccgttg agcaaaagac cgttttcaag aatttccacg gagataccgg cgattgccgc
     1681 ttccatttcg gggccctctt ccggcgagac ttgactcgag taaagagaaa acgcaagagc
     1741 cctgtagaga gcgcccgtgt cgagatagcg catacgaaga aggttcgcaa cctttcgtgc
     1801 aagggtgctt ttccctgctc ccgccgggcc gtcaatggcc gcgacgaaag agacgcaatt
     1861 cattgaaaag cttcctccat ctccgataac tccgcctcgg cctgagctac gaaatccacg
     1921 agatccgaca tgagcatttc gtaatcggaa ataccaagac gctccagcac ttcaggatag
     1981 agatagttct ggagaccgtc ggctccaata ccaactccga gcatcaagca gagggaattc
     2041 gcaagatgca ctacatccac gagattctga tgtttgggtt cagccaggtt gttgggagag
     2101 tgatgatggg cgactgcata tttatacggt tccggcaatc cccacttttc cacaagcatg
     2161 gcccccacca tcgcatggtc gaagccaagc acctgtattt cagcctcaag aaatggaatc
     2221 tgctgttcct cgaccatttt gacgatgatg ccgtatccga agcgaacgta gtcgttcaaa
     2281 acaactttcc cgatatcgtg caaaaggccg ccgacgtagg cttcttcggg aagacctttt
     2341 tttgtgagtt gagctatata gcgggatgaa taggcgacgg tcagggagtg acgccacagc
     2401 ataccccggt ccagcgcgta gcctgcgagc ggtttgtcca ttacagaata caccgaagcg
     2461 gcgaggacaa tactgctgat gtttttgtac ccgaggagcg caatagcctc ggcgacgctg
     2521 gaaatatcac gtgaaatgcc gtatgccgcg gaattggcaa gtttcaacgt ccgaatcacc
     2581 aagccctcat ccctcgaaag actcgcagcg acatcatgag cgctgctgtc gggatcattc
     2641 agctttcgca aagtttccac cacgaactgc gggaatgacc gaatttcttt tatcttaccg
     2701 agaatgcgcg tcttgatcag ttctcgggac cgttcgtcaa ttccttccat cttccgccct
     2761 cctccatacc atcagacaac ccctccggat cggatcgcat tccacagaga tcctcgggac
     2821 atctcgataa actgcccctg ctgcagattt ttcaactcca ttttaccaat tcttttccga
     2881 actagcacga caacatcgca accaatttcg gcggccatac gacgaacttc gcgttttacc
     2941 ccttcggcga gtacaacgga aacccaacaa ccggctggtt ccttgtcaag caacaaaacg
     3001 ttgaccggct tgatcaagcg cccgtcaaga agagatcccg ccatccattt ccgtacttct
     3061 ttccgtgtaa tggcatgatc aagcatcgct tcgtactccc taagaatcct cttcgaaggg
     3121 tgcaatattt cttgagcaaa tgccccgtcg ttggtcagaa tcaacagccc ctggctttcc
     3181 ctgtcaagac gtccaacagg aaaaagccga accgtctgga attccggagg gagcaatcca
     3241 agcaccgtcg gatcataccc atcctctacg gcgcacacaa ctccatccgg tttgttcata
     3301 acgaggtatc gatgttcttg gggctgaaca gtctgtccgt caacgctcac atccatgtca
     3361 ttttcgacgc gatatcctgg cagaaggacg accttgccgt tgatacggac gcgtccggaa
     3421 gcgatgacat cctccacttt ccgtcgggcg cctataccgc acatcgcaag gaatctgttc
     3481 agtcgcgtcc cgttttccac ggcgtactcc tcctccgctc aaagagcttt ataaaatctt
     3541 caggggaaat cacccgttcg tgcgttcctc ctcgtccacg ggaacaaaat gcggttcgtc
     3601 aaccggcgca agttcgtcga gttcctccaa agtgggaagt tccgagattg aaagaacgcc
     3661 gaatgtttct aaaaaacgat ccgttgtgcg atacaggagg ggcgaaccag tcgcctttct
     3721 tcgtcccgct atccggataa gcgcgtgcga gagaagcgtt tcgatcactc tttcgcaccg
     3781 aacacctctt agttcctcga tctcgctgcg cgttaccggt tgattatatg cgatgaccgc
     3841 caatgtctcc actgccgctt tgctgagacg tatcttctgt gcgatcgctg tctcgcggaa
     3901 ggctcccaca atttccgata catcctccgc cgtcgccatc cgccatcctc ctgcggactc
     3961 gattacggta atgccgtgtt catggcgcag gagatacgaa cgaagatcct ccagctctga
     4021 ttttactgcg gaaggagaaa gcgaaagcag cgtagccatc tcatttacag aaacgggaga
     4081 aggcgccgcg aagaggattg cttcgattat cttcgcagta cttgaaagcg tctcaaagcg
     4141 gggtaatgac gatgtcatcg aaaagctcct cctgcagtat ccggattcgt cccatcctgc
     4201 tcatttcaag gagagcgaga agagtcacaa caaaattcgc catcgctcgg ttttcgcgga
     4261 gaaggaatga gagacgaaga gaaccgtctg agttcagttc agaaagaatc tctgaaatct
     4321 tgcgttcaat ttgttcctct tccggtaggg acgacggaac gccgctccaa tcgtcctcat
     4381 tctcgaaaat gtatggcgct gcattttttg tccgtcgttt taaatctctc aagctccacc
     4441 aaagacggca tagagcaaac aggtcgccaa ggtcaaaaga cggttcatcg tcctcttcct
     4501 cgggggcgct gcggaagaaa aaacggtcgc gttcctcttt gagaattgca agatcccgtg
     4561 cagccgaccg atagggacga tacctcgata gtttttcgag gacatcttca ccgggatcgt
     4621 tctcgggttc ttcgctttcg tacagtgatt cgcgtcctgg cagaagggcg cgaatcttgt
     4681 tgagaacaag agaagctgcc ataaacaaaa actccgaaac tacggaaacg gagaccttgt
     4741 tcgtgttggc caggtaggcg ccgtaaatgc ggacgacctg accgacggag atactggcgg
     4801 cttccagctc tctgctttca acgagccagc agagtaaatc aaagggaccg gaaaaaccgt
     4861 cgatctccgc ctggaactct gcgagttctc ccaggcgctc ctccactcct agcctctctt
     4921 gaaaaagccg attgcgttga tcacttcgtc aatcgtacgg cgggcaacct ctccggcttt
     4981 ttccgcaccc tcgcgaagaa tatcggaaag tagatcgggc gacttctcat aaaacgtccg
     5041 ccgttcctga atcggtccca taactctttc aacgtgcgcc tgcagcttct tcttgcagtc
     5101 cacgcagcca attccggctg acctgcatcc ggaagcaatc tcattttttt cgtcttcgtt
     5161 gttgttgaag actttatgga tatcccacac ggggcacttc tcaggatcac cggggtcggt
     5221 gcgccgttct ctcgcaggat ctgtgatcat cggacgaatc ttgttccaca tctctgaagg
     5281 cgtatcggca atgttgatgg cgttcccgta ggatttgctc atctttcgtc cgtccgttcc
     5341 gggaaccttt ggcgtcgggg tcaacatgat ttgcggttca ggaaggatat tgccgaaaaa
     5401 ggagttgaat ctccgggcca tctcccgcgt aatttccaga tgcgcgctct gatcttctcc
     5461 gactggtact atttccgctt tgtacaggag aatatccgcc gccatgagga caggataccc
     5521 taagaaagca tagttgctca gatccttatt ttggatattg agaatctgct ccttatacgt
     5581 cgggcatctc tggagccatc cgagcggcgt taccattgaa agagcaagat gaagctctgc
     5641 atgctgggga atatgcgact ggacaaaaat tacgcacttt tcaggatcga gcccggatgc
     5701 aagccagtcc agcaaaacct ctttgcagtt ctccgctatc atagagctgt ccgcgtactc
     5761 ggacatgagg gcatgccagt cgacgatgga gtaaaaacat ttatagttct cctgaagctt
     5821 tacccaattc gtcaacgctc ctgcaagatg cccgagatgg agctttcctg taggtctcat
     5881 tccactgaaa acacgctgtg ccataatcaa accacctcga atggaagtat cctctgcgtg
     5941 caaggtttat caagaatcat catgattcgg tactcagtct tcagcaccaa aagaactata
     6001 tcatacaaaa tgcaggctgt gaaacatcca ggatttttgc cttttcgccc gttgcttttg
     6061 aaactgtctc cgcgagcgct gtgagagaaa aacgctccat ttctccgtga tcggcaatga
     6121 aaagagagac tcctttgtca agagcgtcaa gacgctcatg gtatttcata tccgctgtga
     6181 aatacgcttc agcgccgcat gcgaacgcct ccggcaagaa cgttccgccg gagcctccgc
     6241 acagagccag cgtcgacacg cttgttttca tatcgccatg gagttcaagc ctggagagat
     6301 gcagcgcatc ccgtattgca gctccgcact ccagaaagtt cagaggtcgt ggaaactttc
     6361 cggctgcccc gctcccccag gcgcccgttg ttgaaggaag aagcggccgg acttcctgca
     6421 gagagagaac ctctgcaata gcggcattca ctccgccggg agcaacatcc cagttcgtgt
     6481 gaagcgaaaa taccgtcaag ccgtggcgta tggcgaaagc gatctgcgcg ccgggcccct
     6541 ctgcgcagtc tatccgtttc agcgggtgaa agatgagcgg gtggtgtgtg atcaacacgg
     6601 agcaagagtg atcgagcgcc gccttcatgg catcgatc
//
LOCUS       DAOTIL010000034         6583 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminobacteriaceae bacterium isolate AMR_MDS_3026
            NODE_13852_length_6583_cov_4.094822, whole genome shotgun sequence.
ACCESSION   DAOTIL010000034 DAOTIL010000000
VERSION     DAOTIL010000034.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868050
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminobacteriaceae bacterium (wastewater metagenome)
  ORGANISM  Aminobacteriaceae bacterium
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae.
REFERENCE   1  (bases 1 to 6583)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6583)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:19:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,224
            CDSs (total)                      :: 3,198
            Genes (coding)                    :: 3,184
            CDSs (with protein)               :: 3,184
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6583
                     /organism="Aminobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13852_length_6583_cov_4.094822"
                     /isolate="AMR_MDS_3026"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:3034609"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..676
                     /gene="phnD"
                     /locus_tag="P5201_01320"
     CDS             <1..676
                     /gene="phnD"
                     /locus_tag="P5201_01320"
                     /inference="COORDINATES: protein motif:HMM:NF024376.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009897 - external side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0043168 - anion binding [Evidence IEA];
                     GO_function: GO:0043225 - ATPase-coupled inorganic anion
                     transmembrane transporter activity [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="phosphate/phosphite/phosphonate ABC transporter
                     substrate-binding protein"
                     /protein_id="HRV97203.1"
                     /translation="RKSRLFVSPDYESLGKMLATGIVDLGWFSPLAYVDAAEKYPLRP
                     LAIPCVKGRPSYQGLVIANKQSGVGALEQLKGKRFAFVDPKSGSGYLYPRLLLQEKGF
                     DPDHFFGEILFLGSHDKVIQAVFEGNVDGGATYTDAWDDAAKTMDLRRLEILARTEDI
                     PKDALAVPASVDAAFAAALAETFLGMKSEGGESGAVMKKVSIDAFVKADDAQYNVLRR
                     AKAMNR"
     gene            complement(735..1478)
                     /locus_tag="P5201_01325"
     CDS             complement(735..1478)
                     /locus_tag="P5201_01325"
                     /inference="COORDINATES: protein motif:HMM:NF024634.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HRV97204.1"
                     /translation="MKRQNLRKALMVIFVLSLPVTMFYLSPLLTLQGAWKGIVTSSLV
                     FVGLSAVLAFFAGRVFCGWICPGGAVQEVLFPANDRRVPGGKLDLIKYGIAAVWLALL
                     VAFFIRAGGLLSVQPLYGIESGFSLATPVSYVMFYVAMGGMVVLSLAVGRRPFCHYGC
                     MLAPFMIAGKYAAKKIGSPRLHLEAESEKCIDCKLCTKNCPMSLDVNAMVRSSEMDSP
                     ECISCAACVDVCPRQVISLVFSSTGSKKS"
     gene            1700..1975
                     /locus_tag="P5201_01330"
     CDS             1700..1975
                     /locus_tag="P5201_01330"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRV97205.1"
                     /translation="MEHISSIITDFIVKNMNERGLSLYRTDEEKILALDDQYETCFKF
                     DLVLSDNDFSCAVLSQGEHGLVLRRRFNIPWTNAAEIREFMEFVRSL"
     gene            1989..2456
                     /gene="msrB"
                     /locus_tag="P5201_01335"
     CDS             1989..2456
                     /gene="msrB"
                     /locus_tag="P5201_01335"
                     /EC_number="1.8.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013047878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033743 - peptide-methionine (R)-S-oxide
                     reductase activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (R)-S-oxide reductase MsrB"
                     /protein_id="HRV97206.1"
                     /translation="MKLTLFVLCACTLLLFAVRAQATAPERVEKSEEEWRAILTPEQF
                     EITRKKGTELAFSGAYWDHHEKGVYACVCCGAQLFSSEAKYDSGSGWPSYWMTIEGAP
                     VAEKRDVSFGMIRTEVLCARCDAHLGHLFTDGPPPTGLRYCINSASLTFIPAE"
     gene            2697..3263
                     /locus_tag="P5201_01340"
     CDS             2697..3263
                     /locus_tag="P5201_01340"
                     /inference="COORDINATES: protein motif:HMM:NF012696.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004089 - carbonate dehydratase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbonic anhydrase"
                     /protein_id="HRV97207.1"
                     /translation="MRRIFDGYARFFSSVDLDEYRALAGGQRPHSLVITCSDSRIVPE
                     ELFDARPGELFVLRNVGNLARLDEPSVAAALDYALLHLGVKNVVVLAHTDCGAVKASK
                     NVDHLDTDGLKRWLGNELFEGVDSEEAGKAAGLRQLRRVEQYPLFKKMADEGSVSASL
                     LFFHIDTCALEVYEDGVWSVPERLAVSK"
     gene            complement(3246..4076)
                     /gene="larE"
                     /locus_tag="P5201_01345"
     CDS             complement(3246..4076)
                     /gene="larE"
                     /locus_tag="P5201_01345"
                     /inference="COORDINATES: protein motif:HMM:TIGR00268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016783 - sulfurtransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent sacrificial sulfur transferase
                     LarE"
                     /protein_id="HRV97208.1"
                     /translation="MPYHAAMKKMERVEALLREMGSAAVAFSGGVDSTLLAAAASRAL
                     GERAVAVTAASPTLPSWEVDDAEQYAALIGIRHVSIPLSELEEPRFAANDRDKCYYCK
                     RFRFELLLKWAKKEGFRWVLDGSNTDDLNDYRPGMRALSELEGVRSPLLEAGLSKSEI
                     RAVSKQWGLPSWQKPAAACLASRIAWGVPITAERLRQVERAEEIVRSFCPPNTQIRVR
                     HHGALARIETPPSALSLLAAPETAARLAPAVLALGFSFVTLDLSGYKMGSLNALFTDG
                     "
     gene            4165..5271
                     /gene="mnmA"
                     /locus_tag="P5201_01350"
     CDS             4165..5271
                     /gene="mnmA"
                     /locus_tag="P5201_01350"
                     /EC_number="2.8.1.13"
                     /inference="COORDINATES: protein motif:HMM:TIGR00420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0016783 - sulfurtransferase activity
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiouridine(34) synthase MnmA"
                     /protein_id="HRV97209.1"
                     /translation="MNIRPSTGFSSSSPSFGQGTVVVLMSGGVDSSVTAWLLQKAGWH
                     VVGVTMKIPGADLSPGSCCGRDAASVALGLGIPHYTAFIEREFEQLVIEPFVESYLRG
                     STPNPCADCNALLKFGLLWDELEDAFGETRLATGHYARVEQEGERFFLSRGKDPSKDQ
                     SYFLYGVARRRLPRLLLPLGGYSKEDVRALAREAALPVAEKSESMEICFAGEGDYRRL
                     LDGRSLPPGLIVDEDGEPLGAHRGIANYTVGQRKGLGISSRDGLHVLRIDPGRNAVIV
                     GPRERAFRRRVRASNVNILFPEPPAKGALLFGKTRSRGEPLPCDLLRMDENVLEVLFH
                     EPQFAPTPGQRLVLYDAGGRVAAGGVIEYSEEAE"
     gene            5271..6083
                     /locus_tag="P5201_01355"
     CDS             5271..6083
                     /locus_tag="P5201_01355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014807584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XdhC/CoxI family protein"
                     /protein_id="HRV97210.1"
                     /translation="MDIGISKRLAKLYEEGKEAVLCMVVEESGSTPRSMGSSMLVFPG
                     GKIEGTVGGGITEHRVIEKALELLGKGTGSMMYRETLSATEAALDGAACGGSMGIYLE
                     VIGRKRELVIFGAGHVGKAIARLGDMTGFAVTIWDERAEFANEENIPWGRTVCCPLEN
                     FFDHVAPFHGDTYVIIVTRGHVLDADVVKLVEGMTAAYMGMIGSRKKIAFVRERLLGQ
                     GVSAGHIDRIYQPVGLPIRAETPEEIAVSAMAEIIAVARGADLPALRSSLTQ"
     gene            6233..>6583
                     /locus_tag="P5201_01360"
     CDS             6233..>6583
                     /locus_tag="P5201_01360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008519700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaJ domain-containing protein"
                     /protein_id="HRV97211.1"
                     /translation="MAVAYKDYYEILGVSRTASQDEIRKAYRKLAKEHHPDVNKNNGS
                     DERYKEINEAYEVLKDPEKRQKYDTLGPNWQQGQDFTPPPGWGGGVHMDFGGDGGGFS
                     DFFRTIFGGFASGGR"
BASE COUNT         1366 a         1849 c         2053 g         1315 t
ORIGIN      
        1 aaggaaaagc cgtcttttcg tctctcctga ttatgagagc ctcggaaaaa tgcttgcgac
       61 aggcatcgtc gatcttgggt ggttcagtcc gctcgcctac gtcgacgccg ctgaaaaata
      121 cccgttgcgt cctctcgcca tcccgtgcgt caaggggcgt ccatcctacc aggggcttgt
      181 gattgccaac aagcagagcg gcgtcggcgc ccttgagcag ctcaagggaa aacggttcgc
      241 tttcgtcgat ccgaaaagcg gctcggggta cctctatccg cgtttgctgt tgcaggagaa
      301 ggggttcgat ccggaccact tcttcggaga gattcttttc ctcggaagcc atgacaaggt
      361 catccaggcc gtttttgagg ggaacgtcga cggcggggcc acctacaccg atgcctggga
      421 tgatgcggcg aaaacaatgg atctccgtcg gctggagatc ctcgccagaa cagaggatat
      481 cccgaaggat gcgctcgccg ttcccgcttc cgtcgatgcg gcgtttgccg ccgctcttgc
      541 ggaaacgttt cttggaatga aatccgaagg gggagagtcc ggcgccgtga tgaagaaggt
      601 ctccatcgac gcttttgtga aggccgacga cgcgcagtac aatgtgcttc gcagggcgaa
      661 ggcgatgaac cgctgaaaaa agaagatcat gcggggcgta cgccgggttt ctccgaagcg
      721 cgccccgtat cgtgtcagct cttctttgat cccgtcgagg aaaaaacgag tgaaataacc
      781 tgtctcggac agacgtcgac gcacgccgcg cacgagatgc actccgggga gtccatttcg
      841 gaggagcgga ccatcgcgtt cacgtccagg ctcatagggc agttcttcgt gcagagcttg
      901 cagtcgatgc acttttcgct ctcggcctcg aggtgcaggc gtggagagcc gatctttttc
      961 gctgcgtact tccccgcgat catgaagggc gccagcatgc agccgtagtg acagaacggc
     1021 cgccgcccca cggcgagaga aaggacgacc atgccgccca tcgcgacgta gaacataacg
     1081 tacgataccg gcgtcgccag agagaacccg ctttcaatgc cgtagagagg ctgcactgag
     1141 agcagtcccc ctgcacggat gaaaaaggcg acgaggagag caagccagac ggcggcgatg
     1201 ccgtacttga taaggtcgag tttcccgccc ggaaccctcc ggtcgttcgc tggaaagagc
     1261 acctcctgca ctgcgccccc cgggcagatc cagccgcaga acacccttcc ggcgaagaag
     1321 gcgagaacag ccgataggcc tacgaagaca aggctcgatg tcacaatgcc cttccaagct
     1381 ccctgcagcg tgaggagcgg ggagaggtag aacatcgtca cagggagcga gagcacaaaa
     1441 atcaccatga gagctttccg cagattctgc cgtttcattg cgatccctcc gtcattgaat
     1501 tgttcactat tatagcagat catacctaat gggggtattc ttatttcccc cgtgaagaca
     1561 tcgttccgtc gcacgccggc gcatactctt ccgcatcgtg atcggatact gttcgcacct
     1621 ttcacccatg acctttttgc gctcagagcg tacaatagta taaatcaccc cggtgcgagg
     1681 ggaagggagg atgtgtgcag tggagcatat ttccagtatc ataactgatt tcatcgtgaa
     1741 aaacatgaac gagagaggct tatcactcta cagaaccgac gaggagaaaa tcttggcgct
     1801 cgacgatcag tacgagacat gtttcaagtt cgaccttgtc ctctcggaca acgacttcag
     1861 ctgcgcggtg ctctcgcagg gggagcacgg gctcgtgttg cgcagacgtt tcaacatccc
     1921 atggacgaac gccgcggaaa tcagggagtt tatggagttc gtccgcagcc tgtaaaggag
     1981 ggaaactcat gaaactgaca cttttcgttc tctgcgcctg tacgctgctt cttttcgccg
     2041 tccgtgcgca ggcgaccgct ccggagaggg tggagaagtc cgaggaggaa tggcgggcaa
     2101 tcctcacccc ggagcagttc gagatcacca gaaaaaaggg gacggagctc gcgttttcag
     2161 gggcgtactg ggatcatcat gagaaagggg tatatgcctg cgtctgttgc ggagcgcagc
     2221 tcttttcctc ggaggccaag tatgattccg gctccggatg gccgagctac tggatgacga
     2281 tcgagggagc gcccgtcgcg gagaagcgcg atgtctcctt cgggatgatc cgcacggaag
     2341 ttctttgcgc acggtgcgac gcgcacctgg ggcacctgtt cacggatggt ccgcccccta
     2401 cggggcttcg ctactgtatc aactccgctt cgctgacctt tattcctgcg gagtgatctg
     2461 ctccggtatt gcttctgcag ccgtccgctt cgtcaggatg cggacggctg tccatttgca
     2521 gggaacgctt cttgccccgg ttggcggaga acagcgcttc ttcatgcacg cgcgggctgg
     2581 acaaaggggc ttgaaatgtg ttcttttcgg gagcataatg ctccattgga tgaacacgcc
     2641 cgcaacgcac gcctgttcgc ctcaatcgat tccatgtgaa agggagtttg tatgccatgc
     2701 gcaggatttt tgatggatat gcccgctttt tttcgtctgt cgacctcgac gagtaccgtg
     2761 cgctcgccgg aggacagcgt cctcattcgc tcgtcatcac ctgttcggac agccggatcg
     2821 tcccggagga gctgttcgac gcccgccccg gagagctctt tgttctgcgg aacgtcggga
     2881 accttgccag gcttgacgaa ccctcggtcg ctgcggctct cgactacgcg ctccttcacc
     2941 tcggagtgaa gaatgtcgtg gtgcttgcgc atacggactg cggagcggtg aaggcctcga
     3001 aaaacgtcga ccatctggat acggacgggc tgaagcgctg gctcgggaac gaattattcg
     3061 agggtgtaga ctccgaggaa gccgggaaag ccgccgggct ccgccagctt cgccgcgttg
     3121 agcagtatcc gcttttcaag aagatggccg acgaaggctc cgtatcggcc tccctcttgt
     3181 ttttccacat cgacacctgc gctcttgaag tgtacgaaga cggggtatgg agcgttcccg
     3241 agcggttagc cgtcagtaaa taacgcgttc agactcccca ttttatagcc ggagagatcg
     3301 agggtgacga aagaaaagcc gagggcgagc acagcgggcg cgagccgtgc cgcagtctcc
     3361 ggcgcggcga gcagcgagag cgcggagggc ggagtttcga ttctcgccag cgctccgtgg
     3421 tgacggaccc tgatttgcgt attggggggg caaaaggagc gtacaatctc ttccgcccgt
     3481 tctacctggc gtaagcgctc cgcggtgatg ggcacgcccc atgcgatccg gctcgcaagg
     3541 caggctgccg cgggtttctg ccacgacgga agcccccact gttttgagac ggcgcgtatc
     3601 tccgatttgc tcaatccggc ttcgagaagc gggctccgta ccccctcaag ctcggagagg
     3661 gcgcgcattc ccggacggta gtcgttcagg tcgtcggtgt tggagccgtc gagcacccag
     3721 cggaacccct cttttttcgc ccacttgagc agcagctcga aacggaatcg cttgcagtag
     3781 tagcacttgt ccctgtcgtt tgcggcaaag cgcggttcct ccagttccga gagcggaatg
     3841 ctcacgtgac ggatgccgat caaagcggcg tactgctcag cgtcgtcgac ctcccatgaa
     3901 ggaagcgtcg gcgacgcggc ggtaacggcc acggccctct cgccaagggc gcgagaagcc
     3961 gctgcggcga ggagcgtgct gtccactccg cccgagaacg ccacggctgc gcttcccatc
     4021 tcccgtaata atgcctccac tctctccatt tttttcatcg cggcgtggta cggcatgctc
     4081 tcgatcctcc cttgtgaaga actttgttct tctctatact acatgagaag agaagacatt
     4141 ccaatgcccg gagggaagtt cctcatgaac attcgaccat cgaccggatt cagctcctca
     4201 tcgccctcct tcggccaggg cacagtcgtc gtcctgatga gcggcggcgt ggacagctcc
     4261 gtgacggcct ggctgctgca aaaagccggg tggcatgtcg tgggtgtcac catgaagatc
     4321 ccgggagcgg atttgtcgcc cggaagctgc tgcggacgcg atgcggcgtc ggtggccctc
     4381 gggctgggaa ttccgcacta tacggccttt atcgaaagag agtttgagca gctcgtcatc
     4441 gaaccgttcg tggagagcta ccttcgcggg agcactccca acccatgcgc cgactgcaat
     4501 gcgctgctga agttcggtct cctgtgggac gagctggagg atgcgttcgg agagacgcgc
     4561 ctcgccaccg ggcactatgc gcgcgtagaa caggagggag agcgcttctt tctctccaga
     4621 gggaaagatc cttccaagga tcagagctat tttctctacg gggtggcgcg tcgtcgcctt
     4681 ccgaggctcc tgcttccgct gggagggtat tccaaggagg atgtgcgcgc tctcgcgagg
     4741 gaggccgccc ttcctgtggc cgagaagagc gagagtatgg agatctgttt cgcgggcgag
     4801 ggggattacc gccgactgct cgacggccgt tcgcttccgc cgggactgat agtggacgag
     4861 gacggcgaac cactcggcgc gcacaggggg atagcaaact acactgtagg gcagagaaaa
     4921 gggcttggaa tctcctcgcg ggacggactg cacgtgctgc ggatcgatcc cgggcgaaac
     4981 gccgtcatcg tcggtcctcg ggagagggct ttcaggcggc gcgtacgcgc gagcaatgtg
     5041 aatatcctct tccccgaacc gccggcgaaa ggggcgctcc ttttcggcaa gacccgctcg
     5101 cggggggagc cgcttccgtg cgatctgctc cgcatggacg agaacgtgct tgaggtctta
     5161 tttcacgagc cgcagttcgc cccgactccg gggcagcggc tggtactcta cgacgccggc
     5221 ggcagagtgg ccgcgggggg cgtcattgaa tacagcgagg aggcggaata atggatatcg
     5281 gcatttcgaa gaggctggcg aagctctacg aagaaggaaa agaagcggtg ctctgtatgg
     5341 tggtggagga gagcgggtcg actccccgaa gcatgggatc ctcgatgctc gtctttcccg
     5401 ggggaaagat agaagggaca gtcggcggcg gtatcactga gcaccgcgtc atcgaaaaag
     5461 ccctcgaact tctcggcaaa ggaacaggca gcatgatgta cagagagacg ctctccgcta
     5521 ccgaggccgc gctcgacggg gcggcctgcg gaggttccat gggaatatac ctcgaagtca
     5581 tcgggagaaa gcgcgaactc gtcatcttcg gggcaggaca cgtcgggaaa gcgatcgccc
     5641 gtctcgggga catgaccgga ttcgccgtca ccatctggga tgaaagagcg gagttcgcca
     5701 acgaggagaa catcccgtgg ggaaggaccg tctgctgtcc gcttgagaat tttttcgatc
     5761 atgtcgcccc tttccacggg gacacctacg tcatcatcgt tacgcgaggg catgtccttg
     5821 acgcggacgt cgtgaagctg gtcgaaggaa tgactgccgc gtacatgggg atgatcggtt
     5881 ccagaaagaa gatcgccttc gtgcgcgagc ggctgttggg acagggagtc tccgccgggc
     5941 acatcgaccg gatttaccag ccggtaggct tgccgatacg tgcggaaacg ccggaggaaa
     6001 tagcggtttc cgctatggcg gagatcatcg ccgtggccag aggggcggat ctgcccgctc
     6061 tgcgctcatc tcttacacag taaaacagag aaaataaaag gaaaagagcg agcatcggca
     6121 ttttcagcgc ctatttgata ttcgcgccta ttgagaacgc taaaaaaaag tgtacactat
     6181 aaaggtcagt attggttaga atttattcgg tatgaaaggc acggtggtgg gcatggccgt
     6241 cgcatacaag gactattacg agattctcgg cgtgagccgg acggcgtccc aggacgagat
     6301 ccggaaggcg taccggaagc tcgcgaagga gcatcacccg gatgtaaaca agaataacgg
     6361 ctccgacgaa cgctacaagg agatcaacga ggcctacgag gtgctcaagg atcctgagaa
     6421 acggcagaag tacgacactc ttggtccgaa ctggcagcag gggcaggatt ttacgccgcc
     6481 tcccggatgg ggcgggggcg tgcacatgga tttcggcggc gacgggggcg ggttcagcga
     6541 cttcttcagg acgatcttcg gtggcttcgc ctccggcggc aga
//
LOCUS       DAOTIL010000035         6556 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminobacteriaceae bacterium isolate AMR_MDS_3026
            NODE_13934_length_6556_cov_3.798031, whole genome shotgun sequence.
ACCESSION   DAOTIL010000035 DAOTIL010000000
VERSION     DAOTIL010000035.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868050
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminobacteriaceae bacterium (wastewater metagenome)
  ORGANISM  Aminobacteriaceae bacterium
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae.
REFERENCE   1  (bases 1 to 6556)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6556)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:19:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,224
            CDSs (total)                      :: 3,198
            Genes (coding)                    :: 3,184
            CDSs (with protein)               :: 3,184
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6556
                     /organism="Aminobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13934_length_6556_cov_3.798031"
                     /isolate="AMR_MDS_3026"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:3034609"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(38..868)
                     /locus_tag="P5201_01365"
     CDS             complement(38..868)
                     /locus_tag="P5201_01365"
                     /inference="COORDINATES: protein motif:HMM:NF012730.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005216 - monoatomic ion channel activity
                     [Evidence IEA];
                     GO_process: GO:0034220 - monoatomic ion transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ion transporter"
                     /protein_id="HRV97212.1"
                     /translation="MNVKKRVWEIVEVAKPGDTVSRVFDIFILTLIIVNVISVIIGSV
                     NQIYDRWGRALDLFEFLSVVIFTGEYVARLWACTVDPRYSGAVRGRIRFALRPMSIID
                     LLAILPFYLPFWSVDLRSMRVLRLLRILRIAKAGRYFTSFGLIKDVLYTNKEELILTT
                     AMMGLLLVVSSSILYYCENEAQPEAFASIPHTMWWAIATLTTVGYGDIAPITGIGRTF
                     ASIIAVIGIGMFALPAGILGAGFVEAIHRRKKREEAAQKKEEDEERGNYCPHCGKRLR
                     "
     gene            1010..1714
                     /locus_tag="P5201_01370"
     CDS             1010..1714
                     /locus_tag="P5201_01370"
                     /EC_number="5.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014162570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047689 - aspartate racemase activity
                     [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid racemase"
                     /protein_id="HRV97213.1"
                     /translation="MTTIRRPDTVLGVLGGMGPAAAAEFLRLLAERAPVSSDQEHAIT
                     YLLSDPQVPDRSSAILGVGEDPTARLKDDLLTLTRWGANLLAVPCNTAHFFIDRFKDE
                     LPVPLVHIIEETVRAAATLSPKGAWLTATLGTMRSGLYQKEAERQGYSFFIPPEEAQR
                     QVQQSIELVKRGRKDEGGRLLKGVVEGLWRERDLPVCAACTELPLAYDASGLPQERTV
                     SSLGALCGACLRRIYG"
     gene            complement(1750..2919)
                     /locus_tag="P5201_01375"
     CDS             complement(1750..2919)
                     /locus_tag="P5201_01375"
                     /inference="COORDINATES: protein motif:HMM:NF012376.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HRV97214.1"
                     /translation="MRLFSIVIICTLFALYSYIYFKLRSAIGPGWKWTALYTLLSFFI
                     IMGSRILWIVNLEAYPGLRKILSCSVYMGMAFFFILFTAFFFIDLLRFLVWLTDLLLS
                     ACFSDLFPSPKMRAVLAIGFAFFACTYGWFEALAVRPVYITIATDRLPRGVDKLRIAH
                     ITDVHLGWVIQEERLDRILDVVRAAQPDMLVSTGDLVDGDMEEREAEVALFRGLNLPL
                     GMYAVTGNHEYYAGIDQAIAFKEHAGMQLLRNEALRVGGIILAGMDDPTALRFGGTPT
                     PEIDVLAPLPENRFVLLLKHQPTVDPDSIGLFDLQLSGHTHRGQIWPFYWLTRMVYEY
                     RPGLRALAPSKSGEAYASGAARESRVFVSNGSGTWGPPIRFFAPPQVGIIDIVRK"
     gene            complement(3053..4255)
                     /gene="rpoD"
                     /locus_tag="P5201_01380"
     CDS             complement(3053..4255)
                     /gene="rpoD"
                     /locus_tag="P5201_01380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009201528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoD"
                     /protein_id="HRV97215.1"
                     /translation="MENDMLNEKEILEDKEILEEKDVSKKDELSREDMAEYLGRVREL
                     IAEGHGKGFVTQKDIERHIPLENWSSEILDNVVSNLMELGIEVMDDDAKEPAPAQASA
                     AAADDDAVAPPSYDLELGKLDDIPLTDPVRMYLREIGKVPLLDAAEEVELAKRVEAGE
                     DLAKKKIVDANLRLVVSIAKKYIGRGMLFLDLIQEGNLGLIRAVEKFDYRKGFKFSTY
                     ATWWIRQAITRAIADQARTIRVPVHMVETINKMVRISRQLVQRLGREPTDDEIAAEME
                     IEPIKVEEIRRIAQLPVSLETPIGEEEDSQLGDFIEDRELPNPEEAAAGHLLHEQIEE
                     MLDALSEREREVLHYRFGLEDGRSYTLEEVGKRFGVTRERIRQIEAKALRKLRHPSRS
                     KKLKDFLD"
     gene            complement(4369..5040)
                     /locus_tag="P5201_01385"
     CDS             complement(4369..5040)
                     /locus_tag="P5201_01385"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRV97216.1"
                     /translation="MTLLPYWDSNVDMEIASWFPLRMDYEPVSVNAHHSRLLSDIAAF
                     SLRMNSEFFCLTSLQSGYVGTLLRDSMIDPGRLNRIELGFTMLFGYSTSMESLLSSLL
                     DDESDVEAGVSFCCLWDRDIMLESFKAIVLVSSNPLFDEDEFLSRLAALLEKNSVPSQ
                     ANLPFFAEKFGYRRFFIPYETDNLVHLEISRLLYEMEFEETRKWVGPSPEVAFPPFFG
                     INLTV"
     gene            complement(5037..5966)
                     /locus_tag="P5201_01390"
     CDS             complement(5037..5966)
                     /locus_tag="P5201_01390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012870302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein"
                     /protein_id="HRV97217.1"
                     /translation="MQEVKKVITEVGTNEWQVVVFFLGDQTFAINVDKTREILRWPGC
                     RTIPESHPSMIGITSVRGEVLPLLDLRIHLGIEAKTPLDSSKVIIAEFNEIKLGFVVD
                     AVERIYRINSEELDSTLTGTFLGEYVLYVIKRDERNVLLLDYEAIVQMVNPTLSQRYT
                     IDVEKTAELTTPLGDLDQYRILVAEDSPLIRKQIADVLGQGGFHNLVLTSDGKEAWDK
                     MNTLGEHYDLLITDVEMPRLDGLALVRRVKEHNDLKKIPIVVFSSIMAQDIKVKAASV
                     GAEAQITKPEIPDMVEHVCRLLLANKEKKGSSK"
     gene            6101..>6556
                     /locus_tag="P5201_01395"
     CDS             6101..>6556
                     /locus_tag="P5201_01395"
                     /inference="COORDINATES: protein motif:HMM:NF013703.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015297 - antiporter activity [Evidence
                     IEA];
                     GO_function: GO:0042910 - xenobiotic transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HRV97218.1"
                     /translation="MRNHQDTLRNEPVGSLLFRLSFPAALGMAVQASYSVVDAFFVGW
                     GVGHEGIAAVSMAFPLQMLLMAAAQTLGVGGASLLSRSLGAERRKRAERTLGTVFTCT
                     WILGILFAAVAIPSAPFVLYFLGAGEGVLPLASEYARALFRGAPFFAFSI"
BASE COUNT         1235 a         1863 c         2061 g         1397 t
ORIGIN      
        1 cgcatcgccc ctgatcagcg tagccggctt tcccgcctca ccgcaggcgc ttgccgcagt
       61 gcgggcagta gttgccccgc tcttcatcct cctctttttt ctgcgcggcc tcttcgcgct
      121 tcttgcgccg atggatggcc tcgacaaatc ccgcgcccag gatgcccgcg gggagagcga
      181 acatgccgat gccgatgacg gcgatgatgc tggcgaaggt tcttccgatg cctgtgatcg
      241 gggcgatgtc gccgtatccg acggtagtga gcgtggcgat ggcccaccac atggtgtgcg
      301 ggatgctggc gaaggcttcc ggctgggcct cgttttcgca gtagtagagg atgctcgacg
      361 agacgacgag cagcagcccc atcatggcgg tggtgaggat gagttcttct ttgttcgtgt
      421 acagcacgtc cttgatgagg ccgaacgatg tgaagtagcg ccccgccttg gcgatccgca
      481 ggatgcggag gaggcgcagc acgcgcatgg agcgaaggtc gacgctccag aaggggaggt
      541 agaagggaag gattgcgagc aggtcgatga tggacatggg gcgcagcgca aagcggatgc
      601 gtccccggac ggcgccggag tagcgggggt cgacggtgca ggcccacagt ctggcgacgt
      661 actccccggt gaagatgacg acggagagga attcgaacag gtcgagcgcc cttccccacc
      721 tgtcgtagat ctggttgacg gagccgatga tgacggagat cacgttgacg atgatgagcg
      781 taagaataaa gatgtcgaac accctgctga cggtatcgcc cggcttggcg acttcgacta
      841 tttcccagac ccgctttttg acgttcactt ctatcctctc ctctcccgaa gtgcgccaaa
      901 gatacagcat gtgcgcgaaa ggagtcaagg agagtttttt cttgctccct cttcctgaaa
      961 aacgcatacc atgtataata cacggagcat attttccgga ggtgttttta tgacgaccat
     1021 acgcagaccg gatacggttc tgggggttct cggcgggatg ggacctgcgg cggctgcgga
     1081 gtttttgcgg ctgcttgcgg aacgcgctcc cgtttcgtcc gaccaggagc acgctataac
     1141 gtatcttctt tcggatccgc aggttcccga ccggagcagc gcgattctcg gcgtcggaga
     1201 agatccgacc gcgcgtctca aggacgacct gctgacgctg acgcgctggg gggcgaatct
     1261 tttggccgtg ccgtgcaaca cggcgcactt tttcatcgac cgtttcaagg acgagcttcc
     1321 cgtccctctg gtgcacatca tcgaggagac ggtaagggcg gcggcgacgc tgagtccgaa
     1381 gggggcctgg ctgacggcga cgctgggaac catgcggagc gggctttatc agaaagaagc
     1441 ggaacggcag gggtactcgt ttttcattcc tccggaggag gcgcagcggc aggtgcagca
     1501 gagcatcgag ctggtgaaga gaggcagaaa ggacgagggg gggcggctgc tgaagggcgt
     1561 tgtggagggg ctgtggcgcg agcgcgatct tccggtgtgc gcggcgtgta cggagctgcc
     1621 tctcgcctat gacgcctcgg ggctgccgca ggagagaacc gtctccagcc tgggggcgct
     1681 ctgcggggcc tgtcttcgaa ggatctacgg gtagcaaaag cgccccctgt tcgacacggg
     1741 ggcgcttttt tattttctca cgatgtcgat gatgcccact tggggcggcg caaagaagcg
     1801 tatcggcggc ccccacgttc ccgatccgtt gctgacgaag acgcggctct cgcgggcggc
     1861 gccggaggcg taggcctctc cgctcttcga cggcgccagg gcgcgtagtc ccggcctgta
     1921 ctcgtagacc atccttgtaa gccagtagaa gggccatatc tggcccctgt gcgtgtggcc
     1981 ggagagctgg agatcaaaaa gaccgatgga atccggatcc actgtgggct ggtgcttgag
     2041 gagcaggacg aagcggttct cgggaagcgg ggcgaggacg tcgatctccg gtgtgggcgt
     2101 tccgccgaag cgtagcgccg tagggtcgtc cattcccgcc aggataatgc cgccgacgcg
     2161 aagcgcctcg ttgcgaagca gctgcattcc ggcgtgctcc ttgaaggcga tcgcctgatc
     2221 gatgcctgcg tagtattcgt ggtttccggt gacggcgtac atccccagcg gcagattcag
     2281 ccctctgaag agtgcaacct cggcctcgcg ctcctccatg tcgccgtcga caaggtcgcc
     2341 ggtgctcacc agcatgtcgg gctgcgcggc tcttaccaca tcaagaatgc ggtcgagccg
     2401 ttcttcctgg atgacccagc ccagatgcac gtccgtgatg tgcgctatcc ggagtttgtc
     2461 cactcccctg gggagcctgt ctgtggcgat agtgatgtac acggggcgaa ctgcaagggc
     2521 ttcgaaccag ccgtaggtgc aggcgaagaa agcgaatccg atcgcgagca ccgcgcgcat
     2581 tttcggggag gggaagaggt cgctgaagca ggcggagagc agtagatcgg tcagccagac
     2641 gaggaaacgc aggaggtcga taaaaaagaa ggccgtaaag aggataaaga agaaagccat
     2701 tcccatgtag acggagcagg aaagaatttt cctgagtccc gggtacgcct ccaggttgac
     2761 gatccagagg atcctcgacc ccatgatgat gaaaaaggag agcagcgtgt agagcgccgt
     2821 ccacttccat ccgggaccga tggcgctgcg cagtttgaaa tagatgtagg agtacagcgc
     2881 aaagagcgtg cagataatca ctatggaaaa gagcctcact tcatcactcc tccttgtctc
     2941 gagagaacat tcgtttccgg gttttattat agcagccggc gcacgtatcg agaaaaagta
     3001 cccggactct cgtgagagga tccgggtacg cgtcggcgct ctctttcagc ggttagtcga
     3061 ggaaatcctt cagcttcttg cttcggctgg ggtgacggag cttgcgcagc gctttggcct
     3121 cgatctggcg gatgcgttcg cgggtgacgc cgaagcgctt gccgacctct tctaaagtgt
     3181 acgaacggcc gtcttccagg ccgaagcggt agtgcagcac ttcgcgctcg cgctcggaga
     3241 gggcgtcaag catctcctcg atctgttcgt gaagcaggtg cccggctgcg gcctcctcgg
     3301 ggttgggaag ctccctgtcc tcgatgaagt cgccgagctg actgtcttcc tcctcgccga
     3361 tgggcgtttc gagggagacg gggagctgcg cgatccggcg gatctcctcc actttgatcg
     3421 gctcgatctc catctcggcg gctatctcgt cgtcggtcgg ctcgcgcccc aggcgctgaa
     3481 cgagctggcg ggagatgcgg accatcttgt tgatggtctc caccatgtgg acggggacgc
     3541 ggatggtgcg cgcctggtcg gcgatcgccc tggtgatggc ctggcgaatc caccatgtgg
     3601 cgtaggtgct gaacttgaac cccttgcggt agtcgaactt ctccaccgcg cggatcaggc
     3661 cgaggttccc ctcctggatg aggtcgagga agagcattcc gcgcccgatg tacttcttgg
     3721 cgatgctcac cacaaggcgc aggttggcgt cgacgatctt cttcttcgct aaatcttctc
     3781 cggcctccac gcgcttggcc agttcgacct cctcggcggc gtcgagcagc ggcaccttgc
     3841 cgatctcgcg gaggtacatg cgaaccgggt cggtaagagg gatgtcgtcc agcttaccga
     3901 gttcgaggtc gtaggagggc ggcgccactg cgtcatcatc tgctgccgcg gcgctggcct
     3961 gcgcgggagc cggttccttg gcgtcgtcgt ccattacttc gattccgagc tccatgaggt
     4021 tggagacgac gttgtcgaga atctcggagc tccagttctc gagagggata tgccgctcaa
     4081 tgtccttctg ggttacaaac cccttaccgt gcccctcggc gatgagttcc cgcactcttc
     4141 cgaggtattc ggccatatcc tcccgcgaaa gctcgtcctt tttggagacg tctttctcct
     4201 caaggatttc cttatcctcc agaatttcct tctcgttgag catatcgttc tccatcgaac
     4261 aaccagtccc ctcttcaaag cgacaatgac gggcctaatg ttcctctcgg gacaccggct
     4321 cgtcgcgtgt gttcttcttc tcttttccga cggacatata ttataccact atacagtcag
     4381 attgatacca aaaaaaggag gaaacgccac ctccggagag gggccgaccc atttgcgggt
     4441 ttcctcgaac tccatctcgt acagaaggcg gctgatctcc aggtggacga ggttatccgt
     4501 ctcgtagggg atgaaaaacc gccggtagcc gaacttctcg gcgaaaaaag gcagattcgc
     4561 ctgcgacggc acgctgttct tttcgaggag cgccgcaagg cgtgagagaa attcgtcttc
     4621 gtcgaacaga gggttgctgc tcacaaggac gatggctttg aagctctcaa gcatgatgtc
     4681 cctgtcccac aggcaacaga aggagacgcc cgcttccacg tcgctctcgt cgtcaagcag
     4741 ggaggagagc aagctctcca tcgacgtgga gtagccgaaa agcatggtga aacccagctc
     4801 gatgcggttc agccgtccgg ggtcgatcat gctgtcccgg agcagcgtgc cgacatagcc
     4861 gctctgaagg ctggtaaggc agaaaaattc gctgttcatt cgaagagaga aggctgcgat
     4921 gtcgctcaaa agcctgctgt ggtgcgcgtt gacggagacc ggctcgtagt ccatcctgag
     4981 aggaaaccac gaagcgatct ccatgtcgac gttggaatcc caatagggaa gcagggtcat
     5041 ttcgacgatc ccttcttctc cttgttcgca agcagaaggc ggcacacgtg ctccaccata
     5101 tccggaattt cgggcttcgt gatctgcgct tcggcgccga cgctcgcggc cttgaccttg
     5161 atgtcctgcg ccataatgga cgagaagacg acgatcggaa tcttcttcag atcgttgtgc
     5221 tccttgacgc ggcggacgag cgcaagcccg tcgaggcgtg gcatctcgac gtcggtgatg
     5281 agcagatcgt agtgctctcc gagggtgttc atcttatccc aggcctcttt gccgtcgctt
     5341 gtgagcacga gattatggaa gcctccctgc ccgagcacgt cggcgatctg ctttcggatg
     5401 agcggcgaat cctccgcgac gagaatgcgg tactggtcga gatcgccgag aggggtcgtc
     5461 agctcggcgg tcttttcgac gtcgatggtg tagcgctgcg agagcgtcgg gttgaccatc
     5521 tggacgatgg cctcatagtc aagaagcagc acgttccgtt cgtcgcgctt gatgacgtag
     5581 aggacatact ctcccaggaa ggttcccgtc agtgtggagt cgagctcttc ggagttgatc
     5641 cgatagatcc gctcgacggc gtccaccacg aatccaagtt tgatctcgtt gaattcagcg
     5701 atgatgacct tgctcgagtc gagcggggtc ttggcttcaa tgccgagatg tatgcgcaga
     5761 tcaagaaggg gaagcacctc tccgcggacc gaagtgatgc cgatcatgga cggatgggat
     5821 tcggggatgg tgcggcatcc gggccaacga agaatctccc tggtcttgtc cacgttgatt
     5881 gcaaaggtct ggtccccaag aaagaacacc acgacctgcc attcgttggt tcccacctcg
     5941 gtgatcactt tcttaacttc ctgcatggac gctccctcct ccgaacggaa cggattgttt
     6001 tccgtgctta ctatatccca tgaaggctcg gaccacaagc ccctcctcgg tacttctgct
     6061 atacttggaa tcgaattcaa attgagaaag gggaatgcgg gtgcggaatc accaggatac
     6121 tctgcgcaac gaacctgtag gttcgctgtt gttccggctt tcctttcccg ccgcgctcgg
     6181 catggccgta caggcttcgt acagcgtggt ggacgctttc ttcgtcggct ggggcgtggg
     6241 gcacgaagga atcgccgctg tttccatggc ctttccgctt cagatgctgc tgatggcggc
     6301 cgcccagact ctcggcgtcg gcggggcgtc gcttctttcg cggagcctcg gcgcggaacg
     6361 aaggaagcgt gcggaacgga cgctcggaac agtcttcacc tgtacgtgga ttttggggat
     6421 tctcttcgcc gccgtcgcga ttccgtccgc gccgttcgtc ctctacttcc tgggcgcggg
     6481 ggagggggtg cttccgctcg cctccgagta tgcgcgggct ctgttcaggg gcgcgccatt
     6541 tttcgcgttc tccatc
//
LOCUS       DAOTIL010000036         6553 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminobacteriaceae bacterium isolate AMR_MDS_3026
            NODE_13941_length_6553_cov_4.151431, whole genome shotgun sequence.
ACCESSION   DAOTIL010000036 DAOTIL010000000
VERSION     DAOTIL010000036.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868050
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminobacteriaceae bacterium (wastewater metagenome)
  ORGANISM  Aminobacteriaceae bacterium
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae.
REFERENCE   1  (bases 1 to 6553)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6553)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:19:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,224
            CDSs (total)                      :: 3,198
            Genes (coding)                    :: 3,184
            CDSs (with protein)               :: 3,184
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6553
                     /organism="Aminobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13941_length_6553_cov_4.151431"
                     /isolate="AMR_MDS_3026"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:3034609"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1152)
                     /locus_tag="P5201_01400"
     CDS             complement(<1..1152)
                     /locus_tag="P5201_01400"
                     /inference="COORDINATES: protein motif:HMM:NF014073.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter permease"
                     /protein_id="HRV97219.1"
                     /translation="MEILHLLADEAVQVGSSLLGALNPTILALIFGGSLLGLLVGAIP
                     GLTATMALALLINLSYGMPLENAVAFLLAVYIGAVSGGLNAAIMINIPGTPSAAATCL
                     DGFPLAKEGKGGLAIGTGLIASFVGTIFSILVMIFLSPLVYKIALRFGHWELFLLALF
                     GIMICGTLSAGKDPLKGWIVGFLGFAAAMVGMEQIFAYPRLTFNFLQLKGGIPLIPAL
                     IGVFGISEFLAVLQENVAYKIDGKVGKIIPPMKEFIPLIPSAIRSGVIGVVIGAIPGA
                     GEDIGSWLSYDVGKRRSKQGHKFGTGSYEGLVCSEVANNAVIGGAMIPLLTLAVPGSP
                     PSAMFLAAVWLHGVRPGPMLNFENPGFLPLVAAMLILGSFAMLFWGLALA"
     gene            complement(1168..1656)
                     /locus_tag="P5201_01405"
     CDS             complement(1168..1656)
                     /locus_tag="P5201_01405"
                     /inference="COORDINATES: protein motif:HMM:NF018979.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter TctB
                     family protein"
                     /protein_id="HRV97220.1"
                     /translation="MAQKVYKGSGADFITAVCLVLFGAAFSIAALRMRVFNNSLLVSP
                     GLFPLILGAVFILLGLLLLRSAAKRGGWAQASHVLGKEHLHTFATSPRVRKAVVLLLL
                     IVAYVAAVANFPFLWATVGYLLVTFIYLKAMKLHWILLVSFLAAYAITFAFRDLFRIP
                     MP"
     gene            complement(1658..2713)
                     /locus_tag="P5201_01410"
     CDS             complement(1658..2713)
                     /locus_tag="P5201_01410"
                     /inference="COORDINATES: protein motif:HMM:NF015366.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter
                     substrate-binding protein"
                     /protein_id="HRV97221.1"
                     /translation="MKKFVSMVAIALVIVFVIAGGAVAAEKVWEKGTPTVIVGFGAGG
                     GTDTSVRPVVALMSKYLNETINVANMPGAASAVAAEHVLGLPADGYTMFATGSGCFGG
                     FKVKETSNSGWEEWSSWHPFQGPAAILVAANGELKTFQDVIDALKTGKINMGASGFGN
                     GPHVIMQAIAEVAGIKEINYVTFDGDRSIATAVLAGEIEAGIVTFSSAIDMAQAGTIK
                     AVVVMTREPMKITDTITIDPIVKVLPGSDKIPDLGETWPICIRRDAPQEIKDKITEAF
                     LWAIKQPEIEEYTKKKGLNIVGLTGEDADRFISLQISGYAWVLHNSGMTEKSPEEFGI
                     PKLANWNWETEKKKLTK"
     gene            complement(2848..3747)
                     /gene="garR"
                     /locus_tag="P5201_01415"
     CDS             complement(2848..3747)
                     /gene="garR"
                     /locus_tag="P5201_01415"
                     /EC_number="1.1.1.60"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008712533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008679 - 2-hydroxy-3-oxopropionate
                     reductase activity [Evidence IEA];
                     GO_process: GO:0046487 - glyoxylate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxy-3-oxopropionate reductase"
                     /protein_id="HRV97222.1"
                     /translation="MEAILMSTIGFIGLGIMGKPMAKNLLAGGLSLVVYDVVSAAVDE
                     VVAAGAAKGTSPKDVAEKSDIVVTMLPNSPHVREVVCGKNGILEGTKKGQIVVDMSSI
                     APLASRELGAMLAEKGAEMLDAPVSGGQEKAEKGTLAIMVGGSEASYEKVKDILALMG
                     KSTLVGELGAGQTTKLVNQTIVAINIAAVAEGMAFAKKAGVDPEKVFQAIRGGLAGSQ
                     CMEDKAPRMFEGRYNPGFRINLHIKDLTNVLETSRSLHMSMPLAAQLMEMFQNLAGEG
                     HETLDHGALGLFYEKLNSLSLKK"
     gene            complement(3761..4690)
                     /locus_tag="P5201_01420"
     CDS             complement(3761..4690)
                     /locus_tag="P5201_01420"
                     /inference="COORDINATES: protein motif:HMM:NF014798.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase C-terminal domain-containing
                     protein"
                     /protein_id="HRV97223.1"
                     /translation="MLMNLRAAYGEALLALAGENKNVVALDADLCGSTQSVVVEKNYP
                     ERYFEMGIGEQNMISVAAGLALTGKIPFAHSFSVFATGRPFDQIRQSVCLPNLNVKIV
                     GSSCGLSDFGDGATHQSFEDLATMRVLPHMTVLCPADGIEVKWAVRAAAAVDGPVYIR
                     LNRNDLPVLFDEKTPFEIGKPLVVKEGSDVALVCTGTMTSIALDAAALLEQQGISARV
                     VHLGTIKPLCGKSLREALGNVRGIVSCEEHTIYGGLGSAVAEALCEAPLPMKMVGIQD
                     RYGQSTVCYEELLECYGLTKEAVAKAAEALLKK"
     gene            complement(4705..5526)
                     /locus_tag="P5201_01425"
     CDS             complement(4705..5526)
                     /locus_tag="P5201_01425"
                     /inference="COORDINATES: protein motif:HMM:NF012670.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HRV97224.1"
                     /translation="MNQETVSAMKEKARAVRLGIIDALGVDKRGHLGGSMSCADIVAA
                     LYFHKMRHDPKNRFMADRDRFIMSKGHSVLAQYAALAEAGYFPRETLLKTKTLGSILQ
                     GHPERETPGVEANTGSLGQGLSIGVGMALAGRLDKLNYRTWVVLGDGELAEGQVWEAV
                     TAAAAFKLDNLTAIVDMNGIQAVGRTCDRYNLGNIRGKFDAFGWKTFEIDGHDMAQVL
                     QALDDASEVRGAPSAIIAGTVKGKGVSFAENTPAYHNNLLTKEQQEAARREIMAM"
     gene            complement(5523..6272)
                     /locus_tag="P5201_01430"
     CDS             complement(5523..6272)
                     /locus_tag="P5201_01430"
                     /inference="COORDINATES: protein motif:HMM:NF019349.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FadR/GntR family transcriptional regulator"
                     /protein_id="HRV97225.1"
                     /translation="MEPIIPFQRKNRSDMIHEKLHELIREGHWKPGDKFPTEMELAKL
                     FNAGRSTVREALNLLKASNLLKVIPGHGTVVASPSELEHNVLSRHIPDSQTARELFNI
                     VEFRICLEPSTTYLAAERIVPAQSDRLLELAGAVNDDTLTPSGFAETDIAFHAFLAEI
                     AGNPLCIETMRSLHNFFYKQQIVTSLISTRKKPVAQSHIAIAKAVADHNAKLAEKEMR
                     AHLYNTRQYLESIVRETHVPKNFLKTSGGES"
BASE COUNT         1196 a         1942 c         2053 g         1362 t
ORIGIN      
        1 ggcgagcgcg agcccccaga agagcatggc gaacgagccg aggataagca tggccgccac
       61 gagggggaga aagccggggt tttcgaagtt gagcatcggg ccggggcgga cgccgtgaag
      121 ccagacggcc gcaaggaaca ttgccgaggg ggggcttccc ggtacggcga gggtgagcag
      181 cgggatcatc gcgccgccga tgacggcgtt gttcgccact tcggaacaga ccagcccttc
      241 gtagctcccc gtgccgaact tgtgcccctg tttggagcgc ctcttgccca cgtcgtagga
      301 gagccagctc ccgatatcct cgccggctcc cggaatggcg ccgatgacga cgccgatgac
      361 gcccgagcga attgcggacg ggatgagcgg aatgaactcc ttcatcggcg gaatgatctt
      421 cccgaccttt ccgtcgatct tgtacgctac gttctcctga agcacggcga gaaactccga
      481 aatgccgaag acgccgatga gcgccggaat gagcgggatc cctcctttga gctgaaggaa
      541 attgaaggtc aggcgggggt aggcgaagat ctgctccatc cccaccatgg cggcggcgaa
      601 gccgaggaaa ccgacgatcc accctttgag cgggtccttc ccggcggaga gcgtgccgca
      661 gatcatgatg ccgaagaggg ccaggaggaa gagctcccag tgcccgaagc ggagcgcgat
      721 tttgtacacg agcggcgaga ggaagatcat caccagaatg ctgaagatgg tgccgacgaa
      781 ggaggcgatc aggccggtgc cgatggcgag ccctcccttt ccctccttcg ccagcgggaa
      841 gccgtcgaga caggtggcgg cggcggaggg ggttcccgga atgttgatca tgatcgcggc
      901 gttgagtcct ccggagacgg cgccgatata tactgcgaga agaaacgcaa cggcgttctc
      961 cagcggcatg ccgtaggaga ggttgataag cagggcgagc gccattgttg cggtgaggcc
     1021 ggggattgcg cctacgagaa gtcccaagag gctgccgccg aagatcagag cgagaatggt
     1081 cgggttgagc gcccccagca gggaacttcc aacctgaaca gcctcgtcag cgagaagatg
     1141 tagtatctcc atatatccgt gcctccttta cggcatcggg atccggaaga gatcccgaaa
     1201 agcgaaagtg atggcgtagg ccgccagaaa ggagaccagc agtatccagt gcagcttcat
     1261 cgccttgaga tagatgaagg tgaccaggag gtagccgacg gtagcccaga gaaagggaaa
     1321 gttcgccact gcggccacgt acgcgacgat gaggagaaga aggacgaccg ctttgcggac
     1381 tctcggcgac gtcgcgaacg tatgaaggtg ttcctttccc agaacgtgcg aggcctgtgc
     1441 ccaccctcct cgtttggctg cggagcggag aaggagaagc ccgaggagga taaagaccgc
     1501 tccgagtatc agagggaaaa gccccggcga aacgaggagg gagttgttga acacgcgcat
     1561 cctcagggcg gcgatggaga acgccgcccc gaagagcacc aggcaaacgg ccgtaatgaa
     1621 atcggccccg gatcccttgt aaactttttg cgccatgcta cttcgtcagc ttcttctttt
     1681 cggtctccca gttccagttg gccagcttgg gaatgccgaa ttcctcgggc gatttctcgg
     1741 tcattcccga attatgaagc acccaggcgt atccggagat ctggagcgaa atgaagcggt
     1801 ccgcgtcttc gccggtgagg ccgacgatat tgaggccttt cttcttcgtg tactcctcga
     1861 tttcgggctg tttgatggcc cagaggaagg cttcggtaat tttatccttg atctcctgag
     1921 gggcgtcgcg gcggatgcag atgggccacg tctcgccgag gtcgggaatc ttgtcgcttc
     1981 cggggagcac cttgacgatc gggtcgatgg tgatcgtatc ggtgatcttc atcggctctc
     2041 tcgtcatcac gacgaccgcc ttgatggttc ctgcctgcgc catgtcgatg gcggaggaga
     2101 aggtcacgat gccggcctcg atctcgccgg cgaggaccgc cgtggcgatg ctccggtcgc
     2161 cgtcgaaggt gacgtagtta atctctttga tacccgccac ttcggcgatc gcctgcatga
     2221 tcacgtgagg gccgttgccg aagccggaag cgcccatgtt gatcttgccg gtcttcaggg
     2281 cgtcgatgac gtcctggaag gtcttcagct cgccgttcgc cgcgacgagg atggctgcgg
     2341 ggccctggaa gggatgccag gagctccact cctcccaccc tgagttggag gtctctttca
     2401 ccttgaagcc gccgaagcag cccgaaccgg tggcgaacat cgtgtatccg tcggcgggaa
     2461 ggccgaggac gtgctccgcg gcaaccgcgc tcgccgcgcc gggcatgttg gcgacgttga
     2521 tcgtttcgtt cagatacttc gacatcaggg cgaccaccgg acggacggat gtgtccgtgc
     2581 cgccgcctgc gccgaagccg acgatgacgg tgggcgttcc cttttcccat actttctccg
     2641 cggcgactgc gccgcccgcg atgacgaaga cgatgacaag tgcgattgca accattgaaa
     2701 cgaacttctt cacaacgacc actccttttt gatatgaaag gttgtgttgc tcgtgttccg
     2761 gaagcttttc gaacggtgcg cgactggctt gtcacaattt ttcccggcgc atccccccta
     2821 gcatagtctg tccgcgtgcg cggacggtta ttttttcagc gacaggctgt tcagcttctc
     2881 gtagaagaga ccgagggcgc cgtggtccag cgtctcgtgc ccttctccgg cgaggttctg
     2941 gaacatctcc atgagctggg ccgcaagcgg catcgacatg tgcagcgaac ggctggtttc
     3001 gaggacgttg gtgaggtcct tgatatgcag gttgatgcgg aagcccggat tgtagcgccc
     3061 ttcgaacatg cggggcgcct tgtcctccat gcactggctg ccggcaaggc cgccgcggat
     3121 tgcctggaag accttttccg ggtccactcc ggccttcttc gcgaaggcca tcccctcggc
     3181 caccgccgcg atgttgatcg ccacgatggt ctggttgacg agtttcgtcg tctgtcccgc
     3241 gccgagttcg ccgacgagcg tgctcttgcc cataagcgcg aggatgtcct tcaccttctc
     3301 gtaagaggcc tcggagcccc ccaccatgat ggccagggtt cccttctcgg ccttctcctg
     3361 ccctccgctg accggagcgt cgagcatctc cgcgcctttt tcggcgagca tcgctcccag
     3421 ttcgcggctc gcaagcggcg cgatggagct catgtcgacg acgatctgcc ctttcttcgt
     3481 tccttccagg atgccgttct tgccgcagac gacctcgcgg acgtgcgggg agttcggcag
     3541 catggtaacg acgatgtccg acttttccgc gacgtctttc ggtgacgtcc ccttcgcggc
     3601 gcccgccgcg acgacttcat ccaccgcggc ggaaaccacg tcgtaaacga cgagcgacag
     3661 ccctccggcc agaagatttt ttgccatggg cttccccatg atgccgagcc cgatgaatcc
     3721 gattgtgctc attagaatcg cctccatata tgaatatctt ttactttttg agcagtgctt
     3781 ccgccgcctt cgcaactgcc tctttggtaa gaccgtagca ctccagcagc tcctcgtagc
     3841 agacggtcga ctggccgtac cggtcctgga tgcccaccat cttcatcggc aggggcgcct
     3901 cgcagagagc ctccgcgacc gccgatccca gcccgccgta gattgtgtgc tcctcgcagc
     3961 tgacgatccc tcggacgttt ccgagagctt cccggagcga cttcccgcag aggggcttga
     4021 tcgtcccgag atggacgacg cgggcggaga ttccctgctg ttcgaggagc gccgccgcgt
     4081 cgagcgctat cgaggtcatc gtccccgtgc agacgagggc gacatcgctc ccctctttga
     4141 ctacaagcgg cttgccgatc tcgaagggcg tcttctcgtc gaagagcacc ggcaggtcgt
     4201 tgcggttcag acggatatag accggcccgt cgacggccgc cgcggcgcgc acggcccatt
     4261 tcacctcgat cccgtccgcc gggcagagaa cggtcatgtg cggaaggacg cgcatcgtgg
     4321 caaggtcctc gaacgactgg tgcgtggcgc cgtcgccgaa atccgagaga ccgcagctcg
     4381 aaccgacgat cttcacgttg aggttcggca ggcagacgct ctggcgtatc tggtcgaagg
     4441 ggcgccccgt tgcgaagacg gagaaggagt gggcgaacgg tatcttccct gtgagcgcaa
     4501 gtcccgcagc gacggagatc atattctgtt cgccgatccc catctcgaag tagcgctccg
     4561 ggtaattctt ctcgacgacg acggactgcg tggaaccgca gagatccgcg tcgagagcga
     4621 cgacgttttt attctccccc gcgagagcga gcagcgcttc gccgtaggcg gcgcgtagat
     4681 tcatcaacat atcttttctc cccctcacat cgccatgatc tcgcggcggg cggcttcctg
     4741 ctgttctttc gtcagcaggt tgttgtggta ggcgggcgtg ttctccgcga aggagacgcc
     4801 cttccccttg actgttccgg cgatgatcgc ggagggcgct cctctgacct cggacgcgtc
     4861 gtcgagcgcc tggagaacct gcgccatgtc gtgtccgtcg atctcgaagg tcttccatcc
     4921 gaaggcgtcg aacttgccgc ggatattgcc caggttgtag cggtcgcacg tgcgccccac
     4981 cgcctggatt ccgttcatgt cgacgatggc ggtcagattg tccagcttga acgccgcagc
     5041 cgcggtcacc gcctcccaga cctgcccctc ggcaagctct ccgtccccga ggacgaccca
     5101 tgtgcggtag ttcagtttgt cgagtcttcc ggcgagcgcc atgcccacgc ctatggagag
     5161 cccctgtccg agcgagccgg tattcgcctc cacgccggga gtttcgcgct ccggatgccc
     5221 ctggaggatg ctgccgagcg tcttcgtctt cagcagcgtc tccctcggga aataccccgc
     5281 ctccgcaagc gccgcgtact gggcgaggac cgagtgcccc ttgctcatga tgaaacggtc
     5341 gcggtccgcc atgaagcggt tcttcgggtc gtggcgcatc ttgtggaagt agagcgccgc
     5401 gacgatgtcg gcgcacgaca tggagccgcc gagatgcccg cgcttgtcca cgccgagcgc
     5461 gtcgatgatg ccgaggcgga cggcgcgcgc cttctctttc attgctgata cagtctcctg
     5521 attcatgatt cacctccgga tgtttttagg aaattcttcg ggacgtgcgt ctcccgtacg
     5581 atgctttcca ggtactgccg tgtgttgtac aagtgcgcgc gcatctcctt ctccgcaagc
     5641 ttcgcgttat ggtctgccac ggccttcgcg atggcgatat ggctctgggc gaccggtttt
     5701 tttcgggtgg agatgagcga cgtgacgatc tgctgcttgt aaaagaagtt gtgaagggag
     5761 cgcatggttt cgatgcagag ggggttgccc gcgatctccg cgaggaatgc gtgaaaggcg
     5821 atgtccgtct cggcgaagcc cgaaggggtc aatgtgtcgt cgttgactgc tcctgcgagt
     5881 tcgagaagcc tgtccgactg cgcgggaacg atgcgttccg cggcgaggta ggtggtggac
     5941 ggttcaaggc agatgcggaa ttcgacgatg ttgaacagct cccgtgcggt ctggctgtcg
     6001 gggatatgcc gcgagaggac gttgtgttcg agttcggaag gactggcgac caccgtgccg
     6061 tgtcccggga tgaccttcag aaggttcgaa gccttcagca ggttgagcgc ttcgcgaacg
     6121 gtggagcgtc cggcgttgaa gagcttcgcc agctccatct cggtcgggaa tttgtcgccg
     6181 ggcttccagt gcccctcacg tatcagttca tggagctttt catggatcat gtcgctgcgg
     6241 tttttccgct ggaacgggat gattggttcc acgaggagca ctctccttga aaaatccggt
     6301 ttgacgtacg tgttctttat cctatgtctg ttgtctgaca acgttagcac accaaagagg
     6361 cgccgtcaaa tttattctat ttttcacaaa aagcggagat gtgggcgact catcccttgc
     6421 gcctgtcttt tcgtattgac cgtaaggaga gggaggggtg cgtacaataa tcggaagaaa
     6481 atcgctcagt gagcaaaacc catgcgattt ttacgcgttc agaagggagt gcattttttc
     6541 agcggtctcc gtt
//
LOCUS       DAOTIL010000037         6511 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminobacteriaceae bacterium isolate AMR_MDS_3026
            NODE_14075_length_6511_cov_2.857342, whole genome shotgun sequence.
ACCESSION   DAOTIL010000037 DAOTIL010000000
VERSION     DAOTIL010000037.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868050
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminobacteriaceae bacterium (wastewater metagenome)
  ORGANISM  Aminobacteriaceae bacterium
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae.
REFERENCE   1  (bases 1 to 6511)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6511)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:19:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,224
            CDSs (total)                      :: 3,198
            Genes (coding)                    :: 3,184
            CDSs (with protein)               :: 3,184
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6511
                     /organism="Aminobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14075_length_6511_cov_2.857342"
                     /isolate="AMR_MDS_3026"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:3034609"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..484
                     /gene="thyX"
                     /locus_tag="P5201_01435"
     CDS             <1..484
                     /gene="thyX"
                     /locus_tag="P5201_01435"
                     /EC_number="2.1.1.148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012869567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050797 - thymidylate synthase (FAD)
                     activity [Evidence IEA];
                     GO_process: GO:0009265 - 2'-deoxyribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="FAD-dependent thymidylate synthase"
                     /protein_id="HRV97226.1"
                     /translation="SRVTSHQLVRHRIASFSQQSQRYVTMGEPDVVVPPSIAERPELT
                     DGFLEMTRSAHAFYRKLVESGVPKEDARYILPHGWSTRLVMTMNARELHHFFTLRLCR
                     RAQWEIRNLAREMLLAVREAAPLLFETAGPSCVVRGLCEEKSPCGRPFASLEELLDDN
                     "
     gene            498..1436
                     /locus_tag="P5201_01440"
     CDS             498..1436
                     /locus_tag="P5201_01440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006583343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1385 domain-containing protein"
                     /protein_id="HRV97227.1"
                     /translation="MKRLFASMLLFLAEGLEQKRIPVGGQAVIEGVLMKGPERWGLSV
                     RKPDGEIENETWQSFSRTKRMPWKLPIVRGVVTMVEMMTVGFRALNKSAQIAVGEEEK
                     ITPKEFLLSVLVAIGAVVGLFIALPLWLSDLAVTSWGLTQTGKNILEGCVRGLVFIAY
                     VGGIGLWSEIRQVFRYHGAEHKTINAFESGADMTPENIRKFSRIHPRCGTSFLLIVVA
                     VSIVVFSIAGSGSILWRIGSRIVLLPLVMGISYEIIKGASCAGILGRILMAPAMSFQY
                     LTTREPDEKQIEVALTSLETALGRKLSPVAAEAPAE"
     gene            1433..2509
                     /gene="prfA"
                     /locus_tag="P5201_01445"
     CDS             1433..2509
                     /gene="prfA"
                     /locus_tag="P5201_01445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008523241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003747 - translation release factor
                     activity [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 1"
                     /protein_id="HRV97228.1"
                     /translation="MNFQDKLSELERDFEETEAKMADPAFHSNMKELQGLAKRHSKLA
                     PVVSKFREFKKVTVDLEEARSLLHCGDPEMESLAKDELSSKEDRLREIEEEMKLLLLP
                     EDPNDEKSVIVEIRAGTGGEEAALFAANLFRMYTRFAEREGWRTEIVDYNETGIGGYK
                     EIIFRIDGDGVYSKLKFESGVHRVQRVPATEAAGRIHTSAATVAVLPEVEDVDVEIRT
                     EDLKIDTYRSSGAGGQYVNMTDSAVRITHLPTGVVATCQDERSQLKNRVKAMAYLRAK
                     LYDLELQKQNDEVASERRGQIGSGDRSERIRTYNFPQNRISDHRIGMTLYKLDQYLDG
                     DLYELVDAMTMADQSQKLHAIEAN"
     assembly_gap    2540..2549
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <2550..3343
                     /gene="prmC"
                     /locus_tag="P5201_01450"
     CDS             <2550..3343
                     /gene="prmC"
                     /locus_tag="P5201_01450"
                     /EC_number="2.1.1.297"
                     /inference="COORDINATES: protein motif:HMM:TIGR03534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0018364 - peptidyl-glutamine methylation
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="peptide chain release factor N(5)-glutamine
                     methyltransferase"
                     /protein_id="HRV97229.1"
                     /translation="VFSPGLEADVILSVACGVSSALLRARPDFELTEENVSELARITG
                     RRARREPLQYILGSWEFYGRALETPPGVLIPRPETEVLVEKALERLPPEGGVFLDWGT
                     GSGCIALSLLCENASACAIAADCNPRALRVSWTNMKRWGVLRRCLLWHSRTPDDLPVS
                     PASLDMLVSNPPYIPTDRMNTLMEEVRYEPQSALDGGIDGADCYRRLFIAGERMLRRG
                     GSLLFEAGDGEQIAFLAGIAPECFELDGIFEDFSKSPRVIAWRRV"
     gene            3389..4576
                     /gene="trxB"
                     /locus_tag="P5201_01455"
     CDS             3389..4576
                     /gene="trxB"
                     /locus_tag="P5201_01455"
                     /EC_number="1.8.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013048126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004791 - thioredoxin-disulfide reductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-disulfide reductase"
                     /protein_id="HRV97230.1"
                     /translation="MEKRELVIIGAGPAGLSAAIYGRRAGLDVLLLEKGVPGGQINIT
                     EEIENWPGVEHATGPELGRMFRSHAEKFKTEFRDVDVSKVEIRGDAKIVVTNKGEIEA
                     EAVIIATGAYFRRLGCEGEAEHIGQGVSYCAVCDGAFFEGEEIAVVGGGNTAVEEGVY
                     LTNFASKVFIIHRRDEFRADRAAVEHAMSNPKIVPVWNTVVEKIEGDGMVENLVLKNL
                     KTGEVSNLPVAGVFMFVGQSPHDECIRGLVDASKGGWIKTNDAMETSVEGVFAAGDVR
                     DKGLRQVITAASDGAIAAMSASSYINEQVHLRSVLIEPAHVVALFYSSIDREQVKLSD
                     EVEALSQKSGKKVALIDGYKNHRMVEKFGLGSLPAMVELSSGTVTRKASVAAASDAAS
                     FLK"
     gene            4752..5240
                     /gene="fliS"
                     /locus_tag="P5201_01460"
     CDS             4752..5240
                     /gene="fliS"
                     /locus_tag="P5201_01460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009201312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009288 - bacterial-type flagellum
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar export chaperone FliS"
                     /protein_id="HRV97231.1"
                     /translation="MDSKNAQNAQTVYLANKVNTASREQLLLITYDIGIKSCKGAENA
                     LDAGVFDEANKHIQKAQDVLRELMVTLRVEEGGEVAQNLMRLYDFMYLMLVEANVSKD
                     REKISVVRGMLEELRTTWEEAIVKLAEEAGIEKPMPHLQGGAAAKEEARAVPVGGLNI
                     AG"
     gene            5230..5685
                     /gene="flgN"
                     /locus_tag="P5201_01465"
     CDS             5230..5685
                     /gene="flgN"
                     /locus_tag="P5201_01465"
                     /inference="COORDINATES: protein motif:HMM:NF016985.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0044780 - bacterial-type flagellum assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar export chaperone FlgN"
                     /protein_id="HRV97232.1"
                     /translation="MQADLEKKLSSLVSSELALFNELALLVEKEEECVLQEDMECLLN
                     VLQEKQDVISRQEKIHEGWSFISSSLGISEGRDGPSFWSRLGELLGEGADDLKASLSV
                     IRDVAGNVLEQEIRVQGLLEKHVDSLRSQMAQVSRGKKALQGYSKAGGV"
     gene            5682..>6511
                     /locus_tag="P5201_01470"
     CDS             5682..>6511
                     /locus_tag="P5201_01470"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: protein motif:HMM:NF013594.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metalloprotease"
                     /protein_id="HRV97233.1"
                     /translation="MRGSLAMKFNAVRKLLFPVCCLLLLAASSLFADELTKEIALGKK
                     VAAEVEEKWELVTDPARTARLGMLLTRLLPHTTRPLPFEARIVREDMINAFSLPGGIV
                     YFTTGMLDFLRTDAEIAAILAHELVHADRRHVMIQTARSSKISIAALALMIATQGAAG
                     PMILTSLAQVAVTNSYSKDLEREADKEGFRMLVAAGFPPAAMVTSLEAMIFDQLKHPY
                     VDPGVFMTHPELSERVEYILKMSEEMKVPIRRKHALNLLRPAVVESGDHLALTLDGTE
                     V"
BASE COUNT         1416 a         1753 c         1976 g         1356 t
ORIGIN      
        1 gagcagggta acgtctcatc agcttgtccg ccacagaata gcttccttta gccagcagag
       61 ccaacggtac gtgacgatgg gagagcccga tgtcgttgta cccccttcca ttgcggaacg
      121 ccccgaactc acggacggtt ttctcgaaat gaccagatcg gcgcacgctt tctaccggaa
      181 gctcgtcgaa agcggcgttc cgaaggagga tgcccgctac attctgcccc atggctggag
      241 cacacggctt gtcatgacga tgaacgcgcg ggagctccat cattttttca cactccggct
      301 ttgccgcagg gcgcagtggg agattcggaa cctggcgcgg gagatgcttc tcgcggtgcg
      361 tgaggcggcg ccccttctct tcgagacggc ggggccgtcg tgcgtggttc gcggcctctg
      421 cgaggagaag tctccgtgcg gccgcccgtt tgcgtctctt gaggaacttt tggatgataa
      481 ttaagtgtga ggtgaacatg aagagattat ttgcgtcgat gctcctcttc cttgcggaag
      541 ggcttgagca gaagagaata ccggtcggcg gccaggcggt catcgaaggc gttcttatga
      601 agggtccgga gcgctgggga ctttcggtca gaaaaccgga cggagagatc gagaacgaaa
      661 cgtggcaaag cttctcgcgg acgaagcgga tgccgtggaa gcttccgata gtgcgcggcg
      721 tcgtgacgat ggtggagatg atgacggtcg gtttccgcgc gctgaacaag tccgcgcaga
      781 tcgccgtcgg tgaagaagaa aaaataacgc cgaaggagtt tctcctctcg gtccttgtcg
      841 ccattggggc ggtcgtcgga ctgttcatcg ccttgccgct ttggctctcc gatcttgccg
      901 ttacttcgtg ggggctgacg caaaccggaa aaaacattct cgaaggatgt gtcaggggcc
      961 ttgtctttat cgcctacgta ggggggatcg gcctctggag cgagatacgg caagttttcc
     1021 gctaccatgg ggcggagcac aagacgatca acgcctttga atccggggcc gacatgacgc
     1081 ccgagaacat acggaaattt tcgcggattc atccgcggtg cggcacatcg ttcctcctca
     1141 tcgtcgttgc ggtgagcatc gtggtcttct ccatcgcggg aagcggctcc atcctctgga
     1201 gaatcggcag caggatcgtg cttcttccgc tggtcatggg aatttcctac gagatcatca
     1261 aaggagcctc ttgcgccggt attctgggca ggatcctcat ggctccggca atgtccttcc
     1321 agtatctcac gacgcgggag ccggacgaga agcagatcga agtcgccctg acgtcgcttg
     1381 agacggcgct aggccgtaaa ctctcgcctg ttgcggcgga ggcgcctgct gaatgaactt
     1441 ccaagacaag ctgagcgagc tcgaaaggga ttttgaagaa acagaagcca agatggccga
     1501 ccctgccttt cactcgaata tgaaggagct gcaaggactg gcgaagcgcc attcgaagct
     1561 cgctcccgtt gtctccaagt tccgcgagtt taaaaaagta accgttgacc tcgaagaggc
     1621 gcggtcgctg cttcactgcg gcgatccgga gatggagtcg ctcgccaagg atgaactctc
     1681 ctcaaaagaa gatcgtctcc gggagatcga agaggagatg aaactccttc ttctgcccga
     1741 ggacccgaac gacgaaaaga gcgttattgt ggaaatccgc gccgggacgg ggggggagga
     1801 ggccgcgctc tttgccgcaa acctcttccg gatgtacaca cgcttcgcgg agcgcgaggg
     1861 gtggagaacc gagatcgtcg actacaacga gaccggtatc ggcggataca aggagattat
     1921 cttccggatc gacggcgacg gcgtgtacag caagctcaag ttcgagagcg gcgtgcacag
     1981 ggtgcagcgc gttcccgcga cagaggccgc cggaaggata catacctccg cggcgacagt
     2041 cgcggtgctt cccgaggtgg aggatgtcga cgttgagatc cggaccgagg atctgaaaat
     2101 agacacctac cgttcgagcg gcgcgggcgg ccagtacgtc aacatgaccg actccgcagt
     2161 aaggattacg cacctcccga cgggcgtcgt ggctacctgc caggacgaac gctcacagct
     2221 gaagaacagg gtaaaggcga tggcctacct tcgcgccaag ctgtacgacc tcgagctgca
     2281 gaagcaaaac gacgaagtag cctccgaacg cagggggcag ataggctcgg gggaccgttc
     2341 ggagcgtatt cggacctaca atttccctca gaacagaatc tccgatcatc gcatcgggat
     2401 gaccctctac aagctggacc agtatctgga cggcgatctt tacgaactcg tcgacgccat
     2461 gacaatggcc gaccagtcgc agaaactcca cgctatcgag gcgaactagc atgaccgcaa
     2521 ccgaggccag gagaaggctn nnnnnnnnng agtcttttct cccggcctcg aagcggatgt
     2581 gatcctttca gttgcgtgcg gcgtatcgtc cgcgctcctt cgtgcccgac ccgatttcga
     2641 gctgaccgaa gagaatgttt cggaactcgc ccgcatcacc ggacggcgcg ctcgaaggga
     2701 gcctctccag tatattctcg gatcatggga attttacggc cgcgcccttg agacaccgcc
     2761 gggtgtcctg attccgaggc cggagacgga agtgctcgtt gagaaagccc tggaacgcct
     2821 tccacccgaa ggaggcgtct ttctcgactg ggggacgggg agcgggtgca tcgcgctttc
     2881 cctcctctgc gaaaacgctt ccgcctgcgc tatagccgcc gattgcaacc ctcgcgcgct
     2941 tcgagtatcg tggacgaaca tgaagcgctg gggcgttctc cggaggtgcc tcctgtggca
     3001 ttcgcgcact cccgacgatc ttcccgtctc tcccgcatcc ctcgatatgc tcgtaagcaa
     3061 cccgccctat atcccgacgg accgtatgaa cacgctcatg gaggaggttc ggtacgaacc
     3121 gcaatccgcc ttggacggcg gtatagacgg agcggactgt tacaggcggc ttttcatcgc
     3181 aggagaacgg atgctgcgcc gcggagggag tcttctcttc gaggcgggcg acggagaaca
     3241 gattgctttt ttagcgggca tcgctccgga gtgctttgaa ttggatggaa tttttgagga
     3301 tttttcgaag tcgcctcgcg taattgcatg gcgtcgtgta taatttcgaa aagagatcat
     3361 gtgtcttatc tggagggata gtttcgatat ggaaaaaaga gagcttgtaa tcatcggtgc
     3421 agggccggcc ggtttatcgg ccgcgatcta cggcagaagg gccggacttg acgtgcttct
     3481 tctcgaaaag ggcgtgcccg gcggacagat caacatcacc gaggaaattg agaactggcc
     3541 cggagtggag catgccaccg gtccggagct tggcaggatg ttcagaagcc acgccgagaa
     3601 gttcaagacc gaattccgcg atgtcgacgt ttccaaggtc gaaattcgcg gcgacgccaa
     3661 gatcgtcgtc acaaacaagg gcgagattga agccgaagcg gttatcatcg ccaccggggc
     3721 ctatttccgc aggctcgggt gcgagggcga agccgaacat atcggccagg gcgtgagcta
     3781 ctgcgcagtc tgcgacggcg ccttctttga aggcgaggag atcgcggtcg tcggcggcgg
     3841 caacacggcc gtcgaagagg gcgtctacct cacgaacttc gcttcgaaag tcttcatcat
     3901 ccacagaaga gacgagttcc gcgccgatag agcggcagtc gaacatgcca tgtcgaatcc
     3961 gaagattgtc ccggtgtgga acaccgttgt cgaaaagatc gaaggcgacg gcatggtcga
     4021 gaatctggtc ctgaagaacc tcaagaccgg cgaggtctcc aaccttcctg tggcgggcgt
     4081 cttcatgttc gtcggccagt ctccccacga cgagtgtatc cgggggcttg ttgacgcgtc
     4141 gaaaggcggc tggatcaaga cgaacgacgc catggaaaca tccgtcgaag gcgtcttcgc
     4201 ggctggagac gtccgggaca aggggcttcg ccaggtgatt acagcggcct ccgacggcgc
     4261 aatcgcggca atgagcgctt cttcgtatat caacgaacag gtccacttgc gctcggtcct
     4321 catcgagccc gcgcatgtgg tcgcgctctt ctactccagc atcgacaggg agcaggtcaa
     4381 gctctccgac gaggtcgagg cgctctcgca gaagagcggg aagaaggtcg ctctaatcga
     4441 cgggtacaaa aaccaccgga tggtggaaaa gttcggtctc ggttctcttc ctgcaatggt
     4501 tgaactctcc tccggcactg tgaccaggaa ggcttccgtt gccgccgctt cggatgccgc
     4561 atctttttta aagtagtttt tctcaaaggg ggtcgtgtca tgcagcagga tgcggctccc
     4621 tctttttctt cgcccggcct ggagtcgggc aggaaagatt cggtgcgtac tttcaaaatg
     4681 accgctgttc ctggtaaggt tttctctctc ctttaccgaa acaagatgga gagaaaggag
     4741 tgatctccgc aatggattcg aaaaacgcac agaacgccca gacagtctac cttgccaata
     4801 aagtgaacac agcctctcgc gagcagcttc ttctcataac atacgatatc ggcatcaaga
     4861 gctgcaaggg agctgagaac gccttggatg ccggtgtttt cgacgaggcg aataaacata
     4921 ttcaaaaagc tcaggacgtt cttcgtgagc ttatggtgac gctccgtgtc gaagagggcg
     4981 gcgaagttgc gcagaatctg atgcgccttt acgacttcat gtatctcatg cttgtcgagg
     5041 caaacgtgag caaggaccgc gaaaagatct cagtcgtcag agggatgctc gaagagcttc
     5101 ggaccacatg ggaggaagct attgtgaagc ttgcggaaga ggcgggtatt gagaagccta
     5161 tgcctcatct tcagggaggc gccgctgcga aagaggaggc tcgcgctgtc ccggttggag
     5221 ggttgaatat tgcaggctga tctcgaaaaa aaactctcca gccttgtttc ctccgagctc
     5281 gcgctcttca acgagctcgc tcttcttgtg gagaaagaag aggagtgcgt tctccaggag
     5341 gatatggaat gtcttttgaa cgtgctgcag gaaaagcagg acgttatttc ccgtcaggaa
     5401 aaaatccacg aggggtggag cttcatctct tcgtcgcttg gcatctccga agggagagac
     5461 ggcccttcat tctggagccg cctgggagaa cttctcggcg agggcgcgga tgatttgaaa
     5521 gcctctcttt ctgttatccg ggatgtcgcc ggaaatgttc tggaacagga aatccgcgtc
     5581 caggggcttc tggaaaagca tgtcgattcc ctccgaagcc agatggccca ggtgagccgg
     5641 gggaaaaaag cgctgcaagg ctattcaaaa gcaggaggag tatgagaggt tcgcttgcca
     5701 tgaaattcaa tgctgttcgc aaactgctct ttccggtctg ttgcctgctt cttctggcag
     5761 cttcgtctct ttttgccgac gaattgacaa aagagattgc tctcgggaag aaagtcgcgg
     5821 cggaagttga agaaaaatgg gagcttgtaa cggatcccgc gcggaccgcg cgtctcggaa
     5881 tgctcctcac ccgattgctt ccgcacacga cgagacctct tcccttcgaa gctcgtattg
     5941 tacgggagga catgatcaat gcgttcagcc ttcccggagg gattgtctat ttcaccacgg
     6001 ggatgctgga ctttttgcgg acagacgcgg aaattgcggc aatcctggct catgagctcg
     6061 tccatgcgga caggcggcac gtgatgatcc agaccgcccg ttcgtcgaag atttctatcg
     6121 ccgctttggc gttgatgatc gccacccaag gcgccgcagg accgatgatc ctgaccagcc
     6181 ttgcgcaggt tgcggtgacg aactcctaca gcaaggacct ggaacgggag gccgacaaag
     6241 aggggttccg tatgctcgtc gccgcgggct ttcctcccgc agcgatggtc acctcgcttg
     6301 aggccatgat cttcgaccag ctcaagcatc cgtacgtgga tcccggtgta tttatgaccc
     6361 accccgagct ttcggagagg gttgagtata ttctgaagat gtcggaagag atgaaggttc
     6421 cgatccggag aaaacatgcg ttgaaccttt tacgtccagc agtggttgaa tccggggatc
     6481 accttgcgct gacccttgac ggaacggagg t
//
LOCUS       DAOTIL010000038         6503 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminobacteriaceae bacterium isolate AMR_MDS_3026
            NODE_14095_length_6503_cov_3.463244, whole genome shotgun sequence.
ACCESSION   DAOTIL010000038 DAOTIL010000000
VERSION     DAOTIL010000038.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868050
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminobacteriaceae bacterium (wastewater metagenome)
  ORGANISM  Aminobacteriaceae bacterium
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae.
REFERENCE   1  (bases 1 to 6503)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6503)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:19:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,224
            CDSs (total)                      :: 3,198
            Genes (coding)                    :: 3,184
            CDSs (with protein)               :: 3,184
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6503
                     /organism="Aminobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14095_length_6503_cov_3.463244"
                     /isolate="AMR_MDS_3026"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:3034609"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(165..1445)
                     /gene="tyrS"
                     /locus_tag="P5201_01475"
     CDS             complement(165..1445)
                     /gene="tyrS"
                     /locus_tag="P5201_01475"
                     /EC_number="6.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014162961.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004831 - tyrosine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006437 - tyrosyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine--tRNA ligase"
                     /protein_id="HRV97234.1"
                     /translation="MFSDALHVLKERGHVEWCSNEEELHSLFTSEMVTAYVGFDPTAD
                     SLHVGHLIPIMALAWIQRCGHRPIPLAGVGTGLIGDPSGKSKERNLLTIEKVQENVIG
                     VKKQLARFLDFDCGGASAQIINNYDWLGKLTFLDVLREVGKHFSVNSMVSREYVKSRL
                     DDPEKSISYTEFSYVLLQAYDFLHLFQNYGCRLQMGGNDQQGNIISGIDLIRKKTGGE
                     AFGATQPLLLTSSGTKFGKTEEGAVWLDPQKTSPYKFYQFWMNTEDQAVEKLLKLFTF
                     LSLEEIEEIMQVHRSAPERREAQRRLAWEVTGIVHGQDNAAMTKKASGILFGESFEPR
                     ELSLSMLDTLAAEVPSGTVSRVFPALLTDILAEAGACSSKSEVRRLLKGGGLSVNGVK
                     VTEEDASVSEDGILAGKYLFVRLGKKRFFMILFQ"
     gene            complement(1499..3010)
                     /locus_tag="P5201_01480"
     CDS             complement(1499..3010)
                     /locus_tag="P5201_01480"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRV97235.1"
                     /translation="MRKGGVSPGVLLAGVVLFLITALYGGRGPEGRHPSDALPLPAEG
                     TLFLHYEGTKASGLSPLLKAMATVGGERAPTLDALFFVPPLFLNAGETAALLAWEENA
                     LHFYMAARLPKEHTQAISEGVFPKGIAPTLTGVSLQEVSPGIFGVRRKNEDLSLFISV
                     NGGITLFASSPEKMSRMALTLERQAEPIDIAWKLERRWPNHIFFSDGGFFSGIASMEG
                     LPAAKENLLVTAAWRDEDDGGRMEWHLEGLDTIFPPSLLKEFREAHWGGRYTFPEPLV
                     AAVGFTIPERFAAFLEDGRMSEAFGLWENADELIKGLLAGPCILSLCGSSKFLVYSLP
                     GVLLQLPERGREGETFAEAFWKKEWSALVPGVEKLDGFPVGGTTAIPFSIVAAANFRM
                     LQLGLIDRDALRHERFRSIYDYAPLLSDIENAFFWSYLDIPKIASALQDLAKIGQVAA
                     KMGKNLRVSPNALFQAADALKEMGGVTIVMPVPEEGFLEWRITKDAAPTEEME"
     gene            complement(3062..4666)
                     /locus_tag="P5201_01485"
     CDS             complement(3062..4666)
                     /locus_tag="P5201_01485"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014162956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HRV97236.1"
                     /translation="MRLKIIGAAGEVTGSSYLLECGASRVLVDCGIFQGKDDERKNRE
                     LFPFSSVSLNAVLLTHAHMDHSGRIPLLVKEGFKGKVWATLPTLELVKVLWYDSAHLM
                     KEEADWRTKKNSRKGLDPVEPIYTRDDVDDAIKMLSAASYDDKIPVAPGISVRFRDAG
                     HILGSAILEVWLEEEGKQVKVVFSGDLGPQQTVMERNPAIITSADYVIMESTYGDRLH
                     KTNAESRDEFRDVLITALKDNAKVFIPSFVVDRAQRILYELALLKDEGILAGNVPIFF
                     DSPMGVKATEIYKKHMNLLSSEIQQYVSQGKDPFAPGKLKYVSSVEESQAINDVRNAV
                     VVAGSGMANGGRIVHHLKHGIWNPKNHVVFVGYQAQGTLGRRIVEGEKNLRIAGEDVS
                     VKANIHTINGFSAHADRDDLLAWAGNFSTAPLFLITHGEPKSSLAFSETLAAAGMKGV
                     VPVAGQEIELEPNGAGKAVHIAEQPVLLKREEVSTLLAEILLLASGLQNRAVPVQEEG
                     ILSLLRSSKVLLETANEKMNERLRAE"
     gene            complement(4635..6086)
                     /locus_tag="P5201_01490"
     CDS             complement(4635..6086)
                     /locus_tag="P5201_01490"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HRV97237.1"
                     /translation="MRRHLLLFIIFALMLPSFAVLLVSGIGILQHEWAIRSVARSYVQ
                     EMAENVASRVATSEPLGWGMELSMLGIHGFRVFTWGPSIPGWVAVLHADGRLLFSSPG
                     ADIAAVWRPNIPIGRAVEIKDKDGDLYTLAVYPVRGGERFVIAAVAWEQLLGPLARGG
                     MLWSVLIVIMAICILLALWALWRWLIRPLRSLVAELEGLSWGRDLPGESDPRSVGEIG
                     SLRKVLYKLASAAVERTHLRNRYVNDIVRVQEGERSRIARELHDGPLQNITAMIQQVR
                     LSRMSRDEDVVKERLLLAEETAHLVVRELREMCDELSPPWIDLGLEQAMTELSERLAR
                     HYNINIVMEIDDSLELDHEKLLSLFRIFQEAVSNAVRHGKAKEMRVNIFRNADNTIFE
                     MKDDGTGFDTRFNYESLRVEGHRGLANMLERMTLVGGKLEVISQKGDGALIRCILPIA
                     PSEEADCRNSVPKLEGGIPQGNHHETKNYWSRR"
     gene            complement(6083..>6503)
                     /locus_tag="P5201_01495"
     CDS             complement(6083..>6503)
                     /locus_tag="P5201_01495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009201929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HRV97238.1"
                     /translation="AAWNHWASGTALKKLAPLRWLSQNSIFSLPTSKYFGHEEITRAI
                     NTVAKGGFYLDSEVASAMGDGEFTPEQLSAREREVLILASKGFSSKEVAARLFISERT
                     VQTHLASIYDKLGAKNKTEALLLALKYGVVTLEELLE"
BASE COUNT         1543 a         1817 c         1549 g         1594 t
ORIGIN      
        1 accgagcgac tccgttcctc ctccacagaa agaatggaga ttaacgcctt cccgaatcaa
       61 cgctctcgcg agagttccga caactgcggc ttcccacggg cgaagagagt cgcttatgta
      121 ccagtagcaa tccatcaggt atcccctccc cttttacccg gctcctattg aaaaagaatc
      181 atgaagaagc gtttctttcc aaggcgaacg aagaggtact tcccggcgag aataccgtcc
      241 tcggaaacag acgcgtcttc ctccgttact tttactccgt tgacggagag ccctccgcct
      301 ttgaggagac gccgtacttc gctcttcgaa gaacatgcgc ccgcttcggc gagaatatcg
      361 gtcagcagtg cggggaacac gcgggatacc gttcccgaag ggacttcggc agcaagagta
      421 tcgagcatgg agagtgaaag ctcccggggt tcgaacgact ctccgaagag gattccgctg
      481 gcttttttcg tcattgcggc gttatcctgt ccatggacga tgccggttac ttcccaggcc
      541 agccttcgct gcgcttcacg acgttcggga gcgcttcggt gcacctgcat tatctcctct
      601 atttcttcga gagagaggaa tgtgaaaagc ttcaggagct tttccacagc ttggtcttca
      661 gtattcatcc agaactggta aaacttgtag ggggacgttt tttgaggatc aagccagact
      721 gcgccctctt cagtttttcc aaatttcgtt ccagatgaag tcagaaggag cggttgcgtt
      781 gctccgaaag cttctcctcc tgttttcttc cggatgaggt caatgccgga gatgatattt
      841 ccctgctggt cattccctcc catctgtaaa cggcacccgt agttctggaa taggtgcagg
      901 aagtcgtagg cttgtagaag aacatacgaa aactccgtat aggaaatgct cttctccgga
      961 tcgtcgagcc tgctttttac atactccctt gagaccatgg aattgacgga gaagtgttta
     1021 cccacttctc tgagaacatc cagaaacgtc agcttcccta gccagtcata gttgttgata
     1081 atttgagcgg atgcccctcc gcagtcgaaa tcaaggaagc gagcaagctg ttttttcact
     1141 ccaatcacgt tttcctgcac cttttcgata gtgaggagat ttcgttcttt gctctttccc
     1201 gaaggatctc caatcaatcc ggtaccaacc cctgcaagag ggatcggccg atggccgcat
     1261 cgttgaatcc aggcaagagc catgatcgga atcaggtggc caacgtgcag gctgtcagcc
     1321 gtaggatcaa accccacata cgccgttacc atctcgcttg tgaaaagaga gtgcaactct
     1381 tcttcgttgc tgcaccattc gacatgtcct ctttctttga gaacatgaag cgcgtctgaa
     1441 aacatgtact tcccctcccg cttttctgta atgaaccgca atccgccgat agtgaattct
     1501 actccatttc ctcagttggt gcagcgtctt ttgttattct ccattcgagg aatccttctt
     1561 cggggacagg cataacgatt gttacgcctc ccatttcttt tagggcatcg gcggcttgaa
     1621 aaagcgcatt cggactcact ctcaagttct tgcccatttt tgccgctact tgacctattt
     1681 ttgcaagatc ctgaagcgcc gaggctatct tcggaatatc tagataagac cagaaaaaag
     1741 cattttcaat gtccgaaagt aacggcgcat agtcatagat cgaacgaaat ctctcgtgcc
     1801 gtaacgcgtc cctgtcgatc aatccgagct gaagcatacg aaaatttgca gcggccacta
     1861 tggaaaaagg gatggctgta gtgcctccta ccggaaatcc atccaacttt tctaccccgg
     1921 gaacgagagc gctccattct ttcttccaga aggcctctgc aaatgtttct ccttcccgcc
     1981 ctcgttccgg tagctgcaga aggactcctg gaagggaata aacaaggaac ttactgctgc
     2041 cgcacaagga aagaatacaa ggccccgcaa gcagcccctt gatcagttca tccgcatttt
     2101 cccacagacc gaaagcctcg ctcatgcggc cgtcctcgag gaaggccgca aacctctcgg
     2161 ggatcgtaaa tccgactgca gcgacgagag gctcggggaa agtataacgc cctccccagt
     2221 gagcttcccg aaactctttc agcaaggaag gggggaaaat agtatcaagc ccttccaaat
     2281 gccattccat gcgtccgccg tcatcttcgt ctctccatgc cgctgttacg aggaggtttt
     2341 ctttagctgc aggcagcccc tccatcgagg caatcccgga aaaaaaccct ccatcggaaa
     2401 agaaaatgtg attaggccac cgtcgttcca atttccaggc aatatctatt ggttctgcct
     2461 gtcgttccag tgtcagcgcc attctactca ttttttcggg agatgaggca aacagcgtga
     2521 tacctccgtt tacgctgata aaaagagaca ggtcctcatt tttcctgcgt acaccaaaga
     2581 tgcccggaga gacctcctgc aacgacactc cagtaagagt cggcgcaata ccttttggga
     2641 acactccttc agagatggcc tgcgtatgct ctttcggtaa tcttgctgcc atgtaaaagt
     2701 gcagagcgtt ttcttcccag gcgagcaaag cggcagtttc accggcgttt aaaaagagcg
     2761 gcgggacaaa aaataaagcg tccaatgtgg gagcacgctc ccctccgact gtcgccattg
     2821 ctttaagcaa cggcgacagt cctgaagcct ttgtaccttc atagtgaagg aagagcgtgc
     2881 cttcagcagg cagcggaaga gcgtcagagg ggtgacgccc ttcagggccc cgtcccccgt
     2941 acagggccgt aatcaaaaac agaacgacac cggcgagaag aactccgggg gagactccac
     3001 ctttacgcat acctccacct ccgtgcccag atcgatacgg gaaaatacga cctgcgacag
     3061 tctattccgc ccgtaaccgc tcgttcattt tttcgttcgc cgtttcgaga agcaccttgc
     3121 ttgacctcag aagcgagaga attccctctt cctgaacagg gacggcacga ttttgaagcc
     3181 cggatgcaag gagaagaatt tcggcgagca gcgttgaaac ctcttctctt ttaaggagca
     3241 ccggctgttc agctatgtgg accgcttttc ccgccccgtt aggttcgagt tctatctcct
     3301 gcccggcaac aggaacaacg cccttcatgc ctgccgctgc aagggtttct gaaaacgcaa
     3361 gagaagattt cggttcgccg tgagtaatca ggaagagggg cgccgtcgaa aaattacctg
     3421 cccatgcaag cagatcgtca cgatccgcat gcgcagaaaa accgtttatc gtatgaatat
     3481 tcgcttttac tgagacatct tctccagcaa tccgtagatt cttctctcct tctacaattc
     3541 tgcggccaag ggtcccctga gcctgatagc cgacaaaaac gacatgattt tttggattcc
     3601 agatcccgtg cttcagatgg tggacgattc gtcctccgtt tgccatcccg cttccggcga
     3661 ccaccacggc atttctgaca tcattgatcg cctgagattc ttcaacgctc gatacgtact
     3721 tcagtttacc cggagcaaac gggtctttcc cttgacttac gtactgctgg atttccgacg
     3781 aaagaagatt catgtgtttc ttgtagattt ccgtggcttt gacgcccatc ggagagtcaa
     3841 agaaaatggg gacattgccg gccagaattc cttcgtcctt taacagagcc agttcgtaga
     3901 ggattctctg cgcccggtcc acaacgaagc tcgggataaa aacctttgcg ttgtctttga
     3961 gagctgtaat aagaacgtcg cggaattcgt ccctgctctc ggcgttggtc ttgtgcaagc
     4021 gatctccgta cgtggattcc atgatcacgt agtcggccga tgtaataata gccgggtttc
     4081 tttccatgac agtttgttgg ggcccaagat cgccgctgaa gacaaccttg acctgtttcc
     4141 cctcttcctc cagccagact tcgagaatgg cgcttccaag gatatggccg gcatctctga
     4201 atctcactga tattccgggg gcaacaggga tcttgtcatc gtaacttgcg gctgagagca
     4261 tcttgattgc gtcgtcaacg tcgtcacggg tgtatatagg ctcaacaggg tcgagtcctt
     4321 ttctggagtt tttcttcgtc cgccagtccg cctcttcctt cataaggtgt gcggagtcgt
     4381 accaaaggac tttcacgagc tccagtgtag gcagcgtagc ccatactttt cctttaaacc
     4441 cctccttgac gagaagggga attcttcccg aatggtccat gtgcgcgtga gtcaaaagaa
     4501 ccgcgttcaa agaaacagat gagaaaggga acagctcgcg gttttttctt tcgtcgtctt
     4561 ttccctgaaa aataccgcaa tcaacgagta cgcgacttgc tccgcactca agaagatagc
     4621 ttgatccggt aacttcaccg gcggctccaa taatttttag tctcatggtg attcccttgc
     4681 gggatccctc cttccagttt gggaacagaa tttctgcaat cagcctcttc agagggagcg
     4741 attggaagaa tacagcgaat aagggcccca tcgccctttt gtgagatgac ttccagcttt
     4801 cctccgacga gcgtcatacg ttcaagcatg tttgccagtc cccggtgccc ctccaccctg
     4861 agactttcat aattaaaacg cgtatcaaaa ccggtaccgt cgtctttcat ctcgaagatg
     4921 gtattgtccg cgtttctgaa gatgtttaca cgcatctctt ttgccttccc gtgccgaact
     4981 gcattcgata cggcttcctg aaagatacgg aacaacgaaa ggagtttttc atggtccaac
     5041 tccaacgaat cgtctatttc catcacgata ttgatattat aatgacgtgc cagccgttcg
     5101 ctcagctccg tcatagcctg ttcaagcccc agatcaatcc acgggggcga gagttcgtcg
     5161 cacatctccc gcagttcccg aacaacaaga tgcgcggttt cctctgcgag cagaaggcgt
     5221 tcttttacaa catcctcatc ccggctcatt cttgaaagac gaacctgctg tatcatggcg
     5281 gtaatattct gcagaggtcc gtcatgcaat tctcgcgcta tcctggacct ttccccctcc
     5341 tggacgcgga caatatcatt tacgtatcgg ttgcggaggt gcgttcgctc tacagccgcg
     5401 cttgcaagct tgtaaaggac ctttcggagg ctgcctatct ctccaaccga tcttgggtct
     5461 gactcgccgg gaaggtctcg tccccaacta agcccttcga gttcggcgac aagacttcgg
     5521 agcgggcgga taagccatct ccacaatgcc caaagcgcca gcagaataca tatcgccata
     5581 atgacaataa gaacagacca gagcataccc cctcgtgcaa ggggaccaag gagctgttcc
     5641 caggcaacag cggcaataac aaaccgttct cctccccgga cagggtacac ggcaagagta
     5701 tacaaatccc cgtctttatc cttgatctca acggctcttc cgatcgggat gttcggacgc
     5761 catactgcag cgatatccgc tcccggcgag gaaaacagca accttccatc tgcatgaagt
     5821 acagctaccc accccgggat ggacggtccc catgtaaaaa cacgaaatcc gtgtatccca
     5881 agcatgctca gttccattcc ccatccgaga ggttcgctcg tggcaactct tgatgcaaca
     5941 ttttccgcca tctcctgcac gtatgaacgg gctaccgaac gaattgccca ttcgtgctgg
     6001 agtatgccga ttcctgaaac gaggagcacc gcaaacgagg gaagcataag agcaaaaatg
     6061 atgaagagaa ggaggtgcct tctcattcaa gcagttcctc cagggtcacc accccgtatt
     6121 tcagagccag caggagcgct tcggtcttgt ttttcgcacc aagtttgtca taaatggagg
     6181 caagatgcgt ctgaactgtt cgttcgctaa taaagagacg agcggcgacc tctttgctgg
     6241 aaaagccttt cgacgccaga atgaggactt ctctttccct cgccgaaagc tgttccggag
     6301 taaattctcc gtcgcccata gcggaagcaa cctctgaatc gagataaaac ccgcctttgg
     6361 ccactgtgtt gattgccctg gtaatctcct cgtgcccaaa gtatttgctc gttggaagcg
     6421 agaaaatgga gttttgggaa agccagcgca aaggagccaa tttcttcaac gccgtgcctg
     6481 aggcccagtg gttccaggcg gca
//
LOCUS       DAOTIL010000039         6382 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminobacteriaceae bacterium isolate AMR_MDS_3026
            NODE_14434_length_6382_cov_4.557926, whole genome shotgun sequence.
ACCESSION   DAOTIL010000039 DAOTIL010000000
VERSION     DAOTIL010000039.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868050
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminobacteriaceae bacterium (wastewater metagenome)
  ORGANISM  Aminobacteriaceae bacterium
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae.
REFERENCE   1  (bases 1 to 6382)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6382)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:19:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,224
            CDSs (total)                      :: 3,198
            Genes (coding)                    :: 3,184
            CDSs (with protein)               :: 3,184
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6382
                     /organism="Aminobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14434_length_6382_cov_4.557926"
                     /isolate="AMR_MDS_3026"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:3034609"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..571
                     /locus_tag="P5201_01500"
     CDS             <1..571
                     /locus_tag="P5201_01500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013048474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="50S ribosome-binding GTPase"
                     /protein_id="HRV97239.1"
                     /translation="KRALQRDRRRKMDVPVVSLVGYTNSGKSSILRALSGDTSLIAEN
                     RLFSTLDTFMRRVDLPSGRTVLLSDTVGFIRDLPPGLVTAFRTTLEEIVASTFLIVVL
                     DISTTDVQEIRDVVEHTLHEIGAGEIPRLFVLNKIDLTSPEYLDATTARLGIENSQSI
                     ATSVLEKRGLHELLDSMDLFLEKMEVSQI"
     gene            568..1452
                     /locus_tag="P5201_01505"
     CDS             568..1452
                     /locus_tag="P5201_01505"
                     /inference="COORDINATES: protein motif:HMM:TIGR00255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YicC family protein"
                     /protein_id="HRV97240.1"
                     /translation="MIRSMTGFTRETRAFEWGTLTVELSSVNHRYQEISPRLPREMAS
                     LEPLITGRLRQGVGRGKIRCSVELHWAPQFRTATLDATVLSNYHTQLAELAERLGTGE
                     TLPLASLLSLPGVFESPSVLAGIEDELNAALESLLEQAIEHLQAMRSTEGTNLLHAIE
                     EYLNAFESLLNSLEKAWDPKRDQAIEDMRSRITNLLEAMSIEVDQNRVAQEVVILSDK
                     WDIAEEFVRSRSHLKQFAAVLNGKSSEGRKLDFLLQEMNREINTMGSKISDAELRWMV
                     VEAKSLLEKIREQVQNVE"
     gene            1460..1729
                     /locus_tag="P5201_01510"
     CDS             1460..1729
                     /locus_tag="P5201_01510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013048472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF370 domain-containing protein"
                     /protein_id="HRV97241.1"
                     /translation="MTGRLVHIGFGNMLVAERIVAIVHPSSAPIKRLKEEAREDGRLI
                     DATQGRKTRAVLITDSNHVVLSAIQPETIVNRYEEEAEDEKTNDG"
     gene            1707..2294
                     /gene="gmk"
                     /locus_tag="P5201_01515"
     CDS             1707..2294
                     /gene="gmk"
                     /locus_tag="P5201_01515"
                     /EC_number="2.7.4.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005662350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004385 - guanylate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="guanylate kinase"
                     /protein_id="HRV97242.1"
                     /translation="MKKRTTGSLFVLSGPSGAGKGTLRKKMFEKVPGITFSISCTTRE
                     PREGEREGIDYHFISADEFQSRLERGEFLENADVHGHLYGTLREDAVRVLNSGSDILL
                     EIDVQGALQVREKMPGSILLFVAPPSFEELEQRLRDRGTESEERLQIRLRNARWEMEK
                     APLYDYVIINDDIERAAEELEGIILEQRKNREARR"
     gene            2291..2545
                     /locus_tag="P5201_01520"
     CDS             2291..2545
                     /locus_tag="P5201_01520"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: protein motif:HMM:NF013365.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit omega"
                     /protein_id="HRV97243.1"
                     /translation="MKFYDLDSLAEKQGLDNKYILTAAVAARARSLSEQKGRTLEEDN
                     EKFISTALRDFDLGLVRLSSDERESEEAPVAVVSDAHVEE"
     gene            2526..3743
                     /gene="coaBC"
                     /locus_tag="P5201_01525"
     CDS             2526..3743
                     /gene="coaBC"
                     /locus_tag="P5201_01525"
                     /EC_number="4.1.1.36"
                     /EC_number="6.3.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009201066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004632 - phosphopantothenate--cysteine
                     ligase activity [Evidence IEA];
                     GO_function: GO:0004633 - phosphopantothenoylcysteine
                     decarboxylase activity [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0015939 - pantothenate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional phosphopantothenoylcysteine
                     decarboxylase/phosphopantothenate--cysteine ligase CoaBC"
                     /protein_id="HRV97244.1"
                     /translation="MPTWKNKVKIVLGITGGIAAYKAPELVRTFVKSGCEVEVVLTED
                     GEKFVSPMVLSTLAGRRIWRQRDFLSDEYGWRIPHISLADWADVVVVAPCTAETLANT
                     VQGRGKELLGSLLLATRAQVLLCPAMNVNMLSNPATAENIRTLKNRGYAVVDPDSGDL
                     ACGYEGKGRLPSPDVLLEEMWRVACPKKELQRKNVLVTAGPTWEFLDPVRFLSNPSTG
                     KMGVAVARAAWYRGASVTLVLGPVSLTNLSGFEVVRVVSAEEMKEAVLSRSGEMEYIV
                     KAAAVGDYRPASYAGEKIKRSGSDTLRIDLIQNTDIAAALGERKRPGQVLIGFAAESH
                     DLQKNASEKIRKKNLDFIVANDITAPAAGFGADTNTVRILSADGSVSEFAGTKEDVAE
                     EIWNTVLADKQLS"
     gene            4662..5573
                     /locus_tag="P5201_01530"
     CDS             4662..5573
                     /locus_tag="P5201_01530"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRV97245.1"
                     /translation="MPLTHTLLSETKRCLASGKTALWLLDRKGYAGEIACEDCGNAAR
                     CPKCGTAMVWEEKNDRLRCPACASLRSIPQVCPVCRGHFLIGKRPGLEALFPVAKSLE
                     SADQPTLFLEDFKTAGKGAVRSLAGRLSSGGIVVGTRAALALCDIVNTGFAGWIDVDA
                     EVRSIAYQAKFTVFSMVWESLWRGMNHSERVVLLQSRRPGAGWQKGVVQGWKAFWNHE
                     LEERKELGLPPYSYLLEIKTSSLQQKSDMISRMEERGLFPMDPGDPPLHFWVTVCSVS
                     DVQDIIAPFFSIGCSRNGFPEVTVWID"
     gene            5597..>6382
                     /gene="der"
                     /locus_tag="P5201_01535"
     CDS             5597..>6382
                     /gene="der"
                     /locus_tag="P5201_01535"
                     /EC_number="3.6.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013048467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase Der"
                     /protein_id="HRV97246.1"
                     /translation="MPVVSIIGRPNVGKSSLFNRLLGRREAIVDDMPGVTRDRLYGEA
                     EWRDRKFFVVDTGGFLARDENEFVEGMRVQVKLAIEESDLVLFVIDGREGPVWMDEDV
                     ADMLRKSGKPVIVVANKIDDGVHEDDVFQAYTLGFEEVIGISAEHKRYIYDLQDLIVS
                     KLPPEKEQPSETGEIRVAIVGRPNVGKSSILNSLAGEERALVSNIPGTTRDALDTLVT
                     INGTVFNFIDTAGLRRKSRVENDIEYYSFVRTLQAIDRCDVALL"
BASE COUNT         1580 a         1504 c         1775 g         1523 t
ORIGIN      
        1 caagagggcg ctccaaagag atcgccgcag gaagatggat gtaccggttg tttctttggt
       61 cggttataca aacagcggaa aatcttcgat cctgagagca ttaagcggcg acacatcgct
      121 tatcgcggaa aacaggcttt tctcaacact ggatacgttc atgcgcaggg ttgatctccc
      181 ctccgggagg acagttcttc tgtccgatac ggtcgggttt atacgagatc ttcctcccgg
      241 tctggtaaca gcgttccgta cgactctcga agagatagtc gcatctacat ttctcatagt
      301 tgttctcgat atctctacaa ccgacgttca agaaatccgg gacgtggtag agcatacctt
      361 gcacgaaata ggcgcagggg aaattcctcg tcttttcgtc ttgaacaaga tcgacctgac
      421 ctcaccagaa tatttggacg cgacaacggc tcgcttaggc attgaaaact cgcagtcaat
      481 cgcgacaagc gtccttgaaa agcgaggatt acatgaacta ctcgattcaa tggatctttt
      541 tcttgaaaaa atggaggtct cccaaatatg atacgcagca tgaccggatt tacgcgggaa
      601 acccgcgctt ttgaatgggg aacgctcacc gtggaactct cctcggtaaa tcatagatat
      661 caggaaattt cacctcgtct gcctcgggag atggcgtcat tggagccttt gatcacagga
      721 agactgagac aaggcgttgg cagaggtaaa attcgttgtt ctgtggaatt gcactgggct
      781 ccgcagtttc gaaccgccac acttgacgca actgtgcttt cgaattatca tacacagctt
      841 gcggagttgg ccgaacgtct cggaactgga gaaactcttc ctcttgcgtc gcttttatcg
      901 ctgcccggcg tttttgaatc tccttccgtt ctcgcaggca tcgaagatga actgaacgca
      961 gcgcttgagt ctctgttgga acaagccata gaacatcttc aagcgatgcg aagtacggag
     1021 ggaacaaatc tcttgcacgc cattgaagag tacctcaacg cgtttgaatc cctcctcaat
     1081 tctttggaga aagcatggga ccccaagcga gatcaggcta tcgaggatat gcgatcaaga
     1141 attacgaacc ttctggaagc catgtcgata gaggttgacc agaaccgggt tgctcaggaa
     1201 gtagttattc tctccgacaa gtgggatatt gccgaggagt ttgttcgttc acgaagccac
     1261 ctgaaacagt ttgctgccgt gctcaacgga aaatcatcgg aagggcgcaa actcgatttt
     1321 cttcttcaag agatgaaccg ggaaattaac accatggggt cgaagatttc cgacgctgaa
     1381 ctccggtgga tggtcgttga agcgaagtcg ctccttgaaa agatcagaga acaagtacaa
     1441 aacgtggagt gataagaata tgaccggaag gctcgttcac attggttttg gcaatatgct
     1501 tgtcgctgaa cgtatcgtcg ctattgtgca tccgtcctcc gctcctataa agcgcctgaa
     1561 ggaagaggca agagaggacg gtcgcctgat tgacgcgacg caaggccgaa agacacgggc
     1621 agttttgatt acagacagca atcatgtcgt cctctctgcg attcagccag agacgatagt
     1681 caatcgatac gaagaagagg ctgaagatga aaaaacgaac gacgggtagt ctttttgtcc
     1741 tctcaggtcc tagcggggca gggaaaggaa cactccggaa aaaaatgttc gagaaggtgc
     1801 ccggtatcac cttctcgatt tcgtgcacta cccgcgagcc gcgcgaaggt gagcgcgaag
     1861 gaatcgacta tcattttatt tctgcggatg agttccagtc acgtcttgag agaggcgaat
     1921 ttcttgaaaa tgcggatgtc cacggccact tatacggtac tttgcgcgaa gatgcggtgc
     1981 gtgtgctgaa ttcaggaagc gacatcctct tggaaatcga cgttcagggg gctttgcagg
     2041 tcagggagaa gatgcccgga agtatcctgc tgttcgtcgc gccaccctcg tttgaagagc
     2101 ttgaacagcg tcttcgtgac agaggaacgg aatcagagga acgtttacaa attcgccttc
     2161 gaaatgctcg atgggaaatg gaaaaggcgc cgttatatga ttatgtgata attaacgatg
     2221 atattgagag ggccgcagaa gaactcgaag gtatcatcct tgaacagaga aagaacaggg
     2281 aggcgcgaag atgaaatttt atgatttgga ttctctcgca gaaaagcagg gtctggataa
     2341 caagtatatc ctcacggcag cagtagcggc aagagcgcgc tcgctcagcg agcagaaagg
     2401 tcgaacgctt gaagaggaca acgagaagtt catctcgaca gctctgcggg attttgatct
     2461 tggcctagtg cgcctttcat cagatgagcg ggaaagcgaa gaagctcccg ttgccgttgt
     2521 ctcagatgcc cacgtggaag aataaagtaa aaatcgtcct cggcataacg ggcggaatcg
     2581 ccgcttacaa agccccggag cttgtacgaa cctttgtgaa gtccgggtgc gaggtcgaag
     2641 ttgtgttgac cgaggacgga gaaaagttcg tcagtccgat ggtgctttca accctcgcgg
     2701 gaagaagaat ctggcgtcag cgtgattttt tgtcggatga atacgggtgg aggattcctc
     2761 acatttcgct ggcggattgg gcggacgttg tggtggtcgc tccttgtacg gcggaaacgc
     2821 ttgcaaatac agttcaagga agaggtaaag agctactcgg ttctcttctt ctcgcgactc
     2881 gggcgcaggt gcttctctgt ccggcgatga acgtgaatat gctctccaat cctgccacgg
     2941 cggaaaacat ccgaactcta aagaacaggg ggtatgcggt tgtcgatccc gactccggag
     3001 acttggcgtg cggttatgaa gggaaaggcc gccttccctc cccggatgta cttcttgagg
     3061 aaatgtggcg tgtagcctgt cccaagaaag agcttcaaag gaaaaatgtc cttgtcactg
     3121 ccggaccaac ctgggagttc cttgatccgg tgcgttttct cagtaatccg agcaccggaa
     3181 agatgggggt cgccgtggcc cgggctgcct ggtacagagg agcttccgtc acccttgtgc
     3241 ttggtccggt ctccttgacc aatctttcgg gattcgaggt agttcgcgtc gtttccgctg
     3301 aagagatgaa agaggctgtc ctctcacgct ccggggaaat ggagtacatt gtaaaagcag
     3361 ctgcggtagg ggactatcgc cccgcttcct atgcaggaga aaagataaaa cgaagcgggt
     3421 cggatacgct tcggatagat ctcatccaga ataccgacat cgccgccgcc ctcggtgaac
     3481 gaaaaagacc ggggcaggtg ctcattgggt ttgctgccga aagccatgac cttcagaaaa
     3541 atgcctccga aaagatacga aagaaaaatt tagacttcat tgtcgcaaac gatataacag
     3601 cccctgcagc aggcttcggt gcagatacca acactgtccg catactgagt gccgacggct
     3661 ctgtatcgga gtttgcaggg accaaggaag acgtagccga agagatctgg aataccgttc
     3721 tcgcagacaa acagttatcg tgaagaagag gcgcctccat gccatattat gacgttctcg
     3781 ttccaggccc atggtggaac agtctgacgt atacgacaga gttgaacgta ccgctcggcg
     3841 cacgagtcgt cgttccttta ggacgtggag agcgtatcgg ttttgtgcag cgcctcagca
     3901 acgctgttca ggagggagac ggcgcgttct cgattcggcg cataaagagg attattgacg
     3961 acgtttcttt tttcccaccg cctttatggg agctttttga ctggggagga agagtgtttc
     4021 tttgcggaag tggagaactg cttcgtatcg gaattccgtc cgctttcctt tcaccctcgg
     4081 atgccactcc cgtacctctt ccattgcctc ggatggagac caaggctcag gaagagttgt
     4141 ttttttatgc gtgtagtttc caagagcgtt gtaacaaatt cctctctctt ctggaaagaa
     4201 gcgagcgttt tcttgttctt ttccctgagc agggacttgc ctccgcgttt tggaagcagc
     4261 tttccgagtc tcaaaagaaa gagaccattc tctggccgtc ccagggaggg aagaaactca
     4321 ttgcggcatg gattcaagcg cgcaataata taccgagagg aatcatcggg gggcccggcg
     4381 cagtttttgc ccctttacaa cagattgaaa ccattattgt tgatgaggaa agcagcggag
     4441 catatcgctc ctataaaaaa ccgttcatta atgcacggac tcttgccggc aaaagagcca
     4501 ggctggagtc cgccttgctt gtactctcgg ggcgggtccc ctcttcccgt atttttctgc
     4561 gcgggaaacc gcaatctaac gaacgcccgg cacggaataa tgtcaggatc gtcgaccttc
     4621 gggaggcgtt tgcttcctct gttcagggag tgggggactc tttgccgttg acacatacgc
     4681 tcctctcgga gacgaaaagg tgtcttgcct cagggaagac ggcgctctgg cttcttgaca
     4741 ggaaaggata cgctggagaa atcgcctgtg aagactgcgg aaatgccgca cgttgtccca
     4801 aatgcggaac ggcaatggtc tgggaagaaa aaaacgatcg tctgcgctgc cctgcctgcg
     4861 catctcttcg ttccattcct caggtgtgtc ctgtgtgcag agggcacttt cttatcggaa
     4921 aaaggccggg actggaagct ctttttccgg ttgcgaagag tttggaatcc gcagatcagc
     4981 ctacactttt tctggaagat tttaaaaccg ccgggaaagg cgctgtacgt tctctcgctg
     5041 gtcgcctctc ctccggaggt attgttgtag gaacacgtgc ggcgctggct ttatgtgata
     5101 tagtcaatac cggcttcgca gggtggattg atgttgacgc tgaagtgcgc agcatcgcct
     5161 accaggcgaa atttactgtt ttttcaatgg tctgggagtc cttatggcgg ggaatgaacc
     5221 attcggaaag agtcgttctt ttacaaagca gaaggccggg agcgggctgg caaaagggcg
     5281 ttgtgcaggg atggaaagcg ttttggaacc atgagctcga ggaacgaaaa gagctgggcc
     5341 ttcctccgta ctcctatctc ctggaaataa agacttcatc gcttcagcaa aagtccgata
     5401 tgatatcacg gatggaggag cggggactct ttccgatgga tccgggcgac cctccgcttc
     5461 atttttgggt gactgtgtgc tcggtatcgg atgttcaaga tatcatcgct ccgttttttt
     5521 ccataggctg ttccagaaat ggttttcctg aagttaccgt ctggattgat tgagctttat
     5581 cggggaggaa gaggcaatgc cagtagtatc aataattgga agaccgaatg ttggcaaatc
     5641 gtcgctgttc aatcgccttc taggaagacg agaggcaatc gtggacgata tgcccggagt
     5701 aacaagagac aggctttacg gagaggcgga atggcgcgac agaaagtttt tcgtcgttga
     5761 taccggtgga tttcttgcgc gggacgagaa tgagttcgta gaaggaatgc gcgtgcaggt
     5821 gaagctggca atcgaagaga gcgaccttgt actcttcgtc atagacggta gggaaggacc
     5881 cgtctggatg gacgaggacg tggcggacat gcttcggaaa tcaggaaaac cagtcatcgt
     5941 cgttgctaac aagatcgacg acggcgttca tgaggacgac gtctttcagg cctacactct
     6001 cggcttcgaa gaggtcatag gtattagcgc cgagcacaaa cggtatatct atgatcttca
     6061 ggatcttatc gtttcaaaac tgccccccga aaaggagcag ccttcggaaa ccggagaaat
     6121 ccgcgttgcg attgtcggtc gtccaaatgt tggcaaatca agcatactga atagtttggc
     6181 cggagaagag cgggcgttgg tgagcaatat ccccggcacg actcgcgacg ctttagatac
     6241 gctcgttacc atcaacggaa ctgttttcaa cttcatcgat actgccggac tccgcagaaa
     6301 aagcagagtg gagaacgaca ttgaatacta ctcgtttgtc agaaccctgc aagcgattga
     6361 tcgctgcgat gtcgctttac tt
//
LOCUS       DAOTIL010000040         6380 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminobacteriaceae bacterium isolate AMR_MDS_3026
            NODE_14444_length_6380_cov_4.088379, whole genome shotgun sequence.
ACCESSION   DAOTIL010000040 DAOTIL010000000
VERSION     DAOTIL010000040.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868050
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminobacteriaceae bacterium (wastewater metagenome)
  ORGANISM  Aminobacteriaceae bacterium
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae.
REFERENCE   1  (bases 1 to 6380)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6380)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:19:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,224
            CDSs (total)                      :: 3,198
            Genes (coding)                    :: 3,184
            CDSs (with protein)               :: 3,184
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6380
                     /organism="Aminobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14444_length_6380_cov_4.088379"
                     /isolate="AMR_MDS_3026"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:3034609"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..395
                     /locus_tag="P5201_01540"
     CDS             <1..395
                     /locus_tag="P5201_01540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013048806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="SEC-C metal-binding domain-containing protein"
                     /protein_id="HRV97247.1"
                     /translation="WKDHLLAMDELRRGIGLRAIGQKDPLLEYQFESYTLFQSMMERV
                     RESVAELAFRVRVVSERGGASDGSNRREGREFILPFGASQEQQPPSLKHGVEGVQQPV
                     KKGPKTGRNDPCPCGSGKKYKHCCGKNA"
     gene            430..2259
                     /locus_tag="P5201_01545"
     CDS             430..2259
                     /locus_tag="P5201_01545"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRV97248.1"
                     /translation="MNACKKMFSGVLSLLAVGVVLFYSPGESFSALSANIPRMIQECP
                     SFSDYPGSSGVIWLKDVRYSLGADGAMTRDSVFVILARRGIDESWSRWTLPVPEDGGS
                     VEVFSAALYDPGSGRMLAPVLPRKSQSGGVSVVELAFPDLQEEYIISLSYREVFPKRF
                     AVDDFIWVNETLPLWELQVTVDVPAGSDLAVSARGVGDLRRENAGAVERHAWHMVNNL
                     PWTTRTLRGDARSYLAFSTRKGLEPLARMLSAYENVLVPQPSAPVEKLINQQNKLRTG
                     SALISWMNAAPSFQGPFLSSIVRSTIPEEGPWSEWEKVLILNRWMRKAGWESTVHWLT
                     AHALNDGTPASGGSVLRPVLELNLVGISPFFLDLGQSQSPNETPPSLWGKHIYTSAGS
                     SLTGRIVSGSSAAEHRLSVEWSLELSADGEANGRVDVFVRNGWNAFFFPGGSPNAATL
                     KRLVNELLPNLPFTEGTEAVKSIKYGWQATFPVSLRNSIVSGGSMLFPYPAVLPSWLN
                     ELGRWSEEYRMNFPFVIEQNFTLKLPPKTDIVMLPASATRSLEKVKYEESVFHNRRRN
                     TLTAGAKLVLSTDTINDAAGRSLAEAVQRWMSWGTKNLPMRSR"
     gene            2287..3963
                     /gene="argS"
                     /locus_tag="P5201_01550"
     CDS             2287..3963
                     /gene="argS"
                     /locus_tag="P5201_01550"
                     /EC_number="6.1.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008523182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004814 - arginine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006420 - arginyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine--tRNA ligase"
                     /protein_id="HRV97249.1"
                     /translation="MADVTKSLRGIVENALRKLADEKGIPSSDLPEIAFEKPKREGQG
                     DVSTNCAMKLCKLFGEKPLDLAGKIVERLEENEFVAKVEVAAPGFVNFFLSLKWMEKV
                     LSEILLEGKRYGSSSLGEDKKVQVEFVSANPTGPLHVGHGRGAAVGDIIASILSFTGW
                     RVEREYYINDAGLQMDILGRSTQSRYFEIACVPEKAPFPEDGYKGEYIYDIARAVREL
                     EGDRFLSMPLEESRPFFKKFAADAILETIKKDLADFGVKFDVWFSEKSLYERDLVPSA
                     MQTLKERGFAFENEGALWFKSTDFTDEKDRVLIRSNGVPTYFASDITYHKEKFDRGFE
                     KVIDIWGADHHGYVPRMKAGVEALGRNAEDLQVLLIQFVNLLRDGQQIPMSTRSGQFV
                     TLRDVLEEVGVDATRFFFVMRRSDSHLDFDLELAKQASNDNPVYYAQYAHARICSVLR
                     EAEARDVRLPGADEVNMEALSGDEERRLLARLGSFPEEMEKASRELAPHILVSYVLDL
                     AGDFHSFYNACRILGEEEFVMKARLLLVQAAGTVLSTALGLLGVSAPERM"
     gene            3968..4270
                     /locus_tag="P5201_01555"
     CDS             3968..4270
                     /locus_tag="P5201_01555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013048803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septum formation initiator"
                     /protein_id="HRV97250.1"
                     /translation="MLRLRWILLAALLILFAAIMGTSFYLEIQKINRLTVLVDERMAI
                     LVSMSRTVQELREKIAFYGTTEGLAHLAREQYNLSFPGELVFKIEKKKNSLPEGRQ"
     gene            4350..4634
                     /gene="rpsF"
                     /locus_tag="P5201_01560"
     CDS             4350..4634
                     /gene="rpsF"
                     /locus_tag="P5201_01560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013048802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S6"
                     /protein_id="HRV97251.1"
                     /translation="MRPYEMMVLVSAELEDPKEEIAKVEEVVRSLEGEIVKTDFWGKR
                     RLAYPIDKKTEGFYALCSFKLKPDQLGELHRVLGLRPNIYRQMTIRLDEK"
     gene            4647..5135
                     /gene="ssb"
                     /locus_tag="P5201_01565"
     CDS             4647..5135
                     /gene="ssb"
                     /locus_tag="P5201_01565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008521683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HRV97252.1"
                     /translation="MAKGFNKVVLMGNLARDPEVRYTAGKQAVANFPLAVNRTWKDKN
                     GELQESVDFIPVVVWGSTAENCERYLKKGSPAFVEGRLQVRSYEAKDGSGKRYVTEVV
                     AAEVIFLGSKKQDAPSDSFEDEGFGDSIRKDRRFSGGSSDDDFPMDISSVQGEEDADI
                     PF"
     gene            5169..5465
                     /gene="rpsR"
                     /locus_tag="P5201_01570"
     CDS             5169..5465
                     /gene="rpsR"
                     /locus_tag="P5201_01570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015557100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S18"
                     /protein_id="HRV97253.1"
                     /translation="MNEGRGEGRGEGRPEGRGGAGPRGPRKGGKRRPKVCFFCVDKVE
                     HVDYKDVEKLRKYVSERGKIMPRRVTGNCAKHQRQLTEAIKRARYMALLPFSLE"
     gene            5550..>6380
                     /locus_tag="P5201_01575"
     CDS             5550..>6380
                     /locus_tag="P5201_01575"
                     /inference="COORDINATES: protein motif:HMM:NF021506.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2232 domain-containing protein"
                     /protein_id="HRV97254.1"
                     /translation="MTSTRSLVESALLAGFAVVLFLAAHFLPLIGVAFSLLAPAPLVI
                     LGLRHDLKKATLGLGVSTLLVASLLGPLSALFFVLGFGVLGVGLGFLAKRCEKGVEVL
                     LYGILLSLGSKLLLMVIAGKVTGINPFQLDGAEMQSMIDKIFLFYESTGMSKESIAAV
                     REQFAESLRLLPVIFPTILTMAAALDCYLSYTISSFVLKRVGGTPLPPLPPFSMWRFP
                     KSVFGALVASILLSLFGSQSGEWNFALRAETNLRLLVKLQREFQGNAVNWYFFSQKAR
                     F"
BASE COUNT         1390 a         1596 c         1887 g         1507 t
ORIGIN      
        1 gctggaagga ccatctcctt gcaatggacg aactccggag gggaatcggc cttcgagcga
       61 tcggacagaa ggacccgtta ttggaatacc agtttgaatc gtacaccctc tttcagtcga
      121 tgatggagcg cgtgcgggaa tctgttgccg aactggcctt cagggttcgc gtagtgagcg
      181 aacgcggcgg tgcgagcgac ggaagtaacc gccgcgaagg aagagagttt attcttcctt
      241 tcggcgcctc gcaggaacag cagccgcctt cactgaaaca cggagttgag ggcgtacagc
      301 agcctgtcaa aaagggaccg aagacaggga ggaacgaccc ctgtccatgc ggcagcggga
      361 aaaaatacaa gcactgctgc ggtaaaaatg cataacaagg gcgtctggat gcggaaagga
      421 gagtgtgcaa tgaacgcgtg caaaaaaatg ttttcaggtg ttttgtctct acttgccgtt
      481 ggtgtagttc tcttttattc acccggagag agtttttccg cgctttcggc gaacattccc
      541 cgtatgatcc aggaatgtcc gagtttttcc gactacccgg gaagctccgg cgttatctgg
      601 ctgaaagatg tccggtattc tttaggcgcc gacggggcta tgacccgcga ttcggtcttc
      661 gttattcttg cccggcgagg gattgacgaa tcgtggagca gatggacgct tcctgtacct
      721 gaggacggag ggagcgtgga ggtcttttct gcagctcttt acgatcccgg ctccgggcgg
      781 atgctcgctc ccgttcttcc cagaaagagc cagtccggcg gggtttcggt tgtcgagctc
      841 gcttttccgg atcttcagga agagtacatt atctctcttt cctaccggga ggtctttcca
      901 aaacgctttg ccgtagatga tttcatttgg gtgaatgaaa ccctcccgct ctgggagctt
      961 caggttaccg tcgacgttcc tgccggcagc gatcttgcag tctcggcgag aggcgtcggc
     1021 gacctgagga gagaaaatgc cggcgctgtg gaacgccatg cctggcatat ggtgaacaat
     1081 cttccgtgga cgacgcggac gctgcgagga gatgctcggt cctatctggc tttttccacc
     1141 agaaagggac ttgaacctct tgcgagaatg ctttcagcct acgagaatgt actcgttccg
     1201 cagccttcgg ctcctgtgga aaaactgatc aatcagcaga acaagctgcg aacggggagc
     1261 gcgcttattt catggatgaa tgccgcgcct tccttccagg gaccgtttct ttcctcgatt
     1321 gttcgttcga ctatccctga ggaggggccc tggtccgaat gggaaaaggt gcttattctc
     1381 aacaggtgga tgcgaaaagc aggctgggaa agcaccgtac attggctgac cgcgcatgcg
     1441 ctcaatgacg gtactccagc ttcgggcgga tcggttctcc gtcccgtgct ggagttgaac
     1501 ctcgtcggca tctccccgtt tttcctcgat ctcggacaga gccagagtcc gaacgaaaca
     1561 cctccctcac tgtggggaaa gcatatctat acctccgccg ggagcagcct taccggtcgg
     1621 atagtcagcg gaagttccgc tgcggaacac aggctttcgg tcgaatggag ccttgaactt
     1681 tccgccgacg gcgaggcgaa tgggcgtgtg gacgtattcg tccggaacgg ctggaacgcc
     1741 tttttcttcc ccggagggag cccgaatgcg gcgacgttga aacgtctcgt gaatgaactg
     1801 ttaccaaatc ttcccttcac cgaagggacc gaagcggtga agtccatcaa gtacggatgg
     1861 caggcgacgt tccccgtttc gctgaggaac agtatcgtga gcggcggttc catgctcttc
     1921 ccgtatccgg cagtcttacc gtcgtggctg aatgaactgg gccgatggtc ggaagagtac
     1981 aggatgaact tccccttcgt gatcgagcag aacttcacgc tgaaacttcc tccgaagacc
     2041 gatattgtaa tgctgcctgc ttccgcgaca cgctcgctgg aaaaagtcaa gtatgaggag
     2101 tcggtctttc ataaccgccg ccgcaacacg ctgacggcgg gggcgaaact tgtgctgagc
     2161 actgatacca taaacgatgc agcgggaaga agtctcgccg aagccgtcca gcgatggatg
     2221 tcctggggga cgaagaacct tcccatgcgt tcgcgctaaa cgaagagaac ctatggaggt
     2281 ggctatatgg cggatgtaac aaaatccctt agggggatcg ttgaaaacgc gttgcgaaaa
     2341 ctggctgacg agaaggggat cccttcctcg gacctgccgg agatcgcatt tgaaaaacct
     2401 aaacgcgagg ggcagggaga cgtttcgacg aactgtgcga tgaagctctg taaactgttc
     2461 ggcgaaaaac ctttagatct ggccgggaag atcgtcgagc gtcttgagga gaacgaattt
     2521 gttgcgaagg ttgaagtagc cgctcccggt ttcgtcaact tttttctctc gctgaagtgg
     2581 atggagaagg ttctgtcaga gattctcctc gaggggaaaa ggtacggttc ctcttctctc
     2641 ggcgaagaca agaaggtgca ggttgaattc gtcagcgcga atcctaccgg accgcttcac
     2701 gtagggcacg gcaggggggc ggccgtcggc gatatcatag cgtccattct ctcctttacg
     2761 gggtggaggg tagagcggga gtactatatc aacgatgcgg gccttcagat ggacatcctt
     2821 ggacgctcga cacagtcccg ctatttcgag atcgcatgcg ttcctgaaaa ggcccccttc
     2881 cctgaggatg gctataaggg ggagtatatc tacgacatag cgcgcgccgt tcgcgagctt
     2941 gagggggatc gttttctctc catgcctctg gaggaaagcc ggccgttctt caaaaaattc
     3001 gcagccgacg ctattctgga aacgataaaa aaggacttgg ccgatttcgg tgtgaagttc
     3061 gacgtttggt tctcagagaa gagtttgtat gagcgtgatt tagtcccttc agctatgcaa
     3121 accttgaaag agcgcgggtt tgctttcgaa aatgaaggag cgctctggtt caagtcgacg
     3181 gacttcaccg acgagaagga cagggtcctg atcagaagca acggagtccc cacgtacttc
     3241 gcgtcggaca taacgtatca caaagagaag tttgacaggg gcttcgagaa agtcattgac
     3301 atctggggag cggatcatca cggatacgtt ccgaggatga aggcaggcgt tgaagctctt
     3361 ggaagaaatg ccgaggatct tcaggtgctg ctcatacagt ttgtcaatct ccttcgggat
     3421 ggacagcaga tccccatgtc cacgcgatca ggccagttcg tcacgctacg ggacgtactg
     3481 gaggaagtcg gagtggatgc cactcgcttt ttctttgtca tgcgacgaag cgactctcac
     3541 cttgatttcg atcttgaact cgcaaaacag gcatcaaatg acaacccggt gtactacgcc
     3601 cagtatgcgc atgcacgtat ctgcagcgtt ctccgcgaag cggaggctcg tgacgtgcgg
     3661 cttcccggtg cagacgaggt aaatatggag gcgctctccg gagatgaaga aaggcgtctt
     3721 cttgccaggc ttggctcctt tcctgaagag atggaaaaag cttcgcgtga acttgcgccg
     3781 catatattgg tcagctatgt tttggatctc gccggtgatt tccactcctt ctacaacgcg
     3841 tgccgcatac tcggcgagga ggaattcgtg atgaaagcgc gtctgctcct tgttcaggcc
     3901 gccggtacgg tgctttcgac ggcgcttggc ctgcttgggg tttcggcacc cgagaggatg
     3961 tagtgatgtg cttcgcctcc gttggattct gcttgcggcg cttctgattc tttttgcggc
     4021 tataatgggg acatcgtttt atctggagat ccaaaagatc aaccgtctga ccgtccttgt
     4081 cgacgagcgg atggcgatcc tggtctcgat gagccgcact gtgcaggagc ttcgggagaa
     4141 gatagcgttt tacggcacta cggaggggct ggcgcacctt gcccgcgaac agtacaacct
     4201 ctccttcccg ggagagctgg tttttaaaat cgaaaaaaag aagaattcct tgcctgaagg
     4261 gagacaataa tgtataatcc catggcaagt ttttccctgc ccctttcttt gaaaaggggc
     4321 cagagtccag agggaggagg tgaatgagtg tgcggccata tgaaatgatg gtgcttgtga
     4381 gcgccgaact ggaggatccc aaagaggaga tcgcgaaggt tgaagaggtc gtgcgcagtc
     4441 ttgaggggga gatcgtcaag actgatttct ggggaaaaag gcgtcttgcc taccccatcg
     4501 ataagaaaac ggaaggtttt tacgcattgt gcagcttcaa gctgaagccc gatcagcttg
     4561 gagagcttca ccgtgttctt ggtttgcgcc cgaacattta tcgtcagatg acaatccgtc
     4621 ttgacgaaaa gtaaggaggc ggggtcgtgg cgaaagggtt caataaagtc gtactcatgg
     4681 ggaatctggc tcgagatcct gaagtgcggt ataccgccgg aaaacaggcc gttgcgaact
     4741 ttccgcttgc tgtaaacaga acgtggaagg ataaaaacgg cgaattgcag gagagcgttg
     4801 actttattcc tgttgttgtc tgggggtcca ctgctgaaaa ctgtgaacgt tacttgaaga
     4861 aagggagtcc cgctttcgtc gagggcagac ttcaggttcg gagttacgaa gccaaggacg
     4921 gttcagggaa acggtacgtc accgaagtgg ttgccgccga agttattttc ctcggctcga
     4981 agaagcagga cgctccttcc gattcgttcg aggacgaggg gttcggcgac agcatccgga
     5041 aggatcgccg cttcagcggc gggagttcgg acgacgactt cccgatggac atttcctcgg
     5101 tgcaaggcga agaggatgcg gatatcccct tttaggtgtt ttttgaagga ggacagtgca
     5161 caatggcaat gaatgaaggt agaggcgaag gcagaggcga aggcaggccc gaaggcagag
     5221 gcggagccgg tcccagaggg cctcgaaaag gcgggaaacg ccggcctaag gtatgttttt
     5281 tctgcgtaga taaagttgag catgttgatt acaaagatgt cgaaaagctt cgaaagtacg
     5341 tgagcgagcg cggaaagatc atgccgcgcc gtgtgacggg aaattgcgcg aagcatcagc
     5401 gtcagctcac cgaggcgatc aaaagggccc ggtatatggc tctccttccg ttctctctgg
     5461 agtagggcgc ctatctgaaa cgttcttttc ggggggagga gcctggcttc tccccccgtc
     5521 cttttagttt ttcgaaggga ggttgcggtg tgacttcgac acgaagcctg gttgaatcgg
     5581 ctcttctcgc tggttttgcg gtggtgctct ttcttgccgc gcattttctt ccgcttatcg
     5641 gtgtagcgtt ctctcttctt gcccccgccc ctcttgtaat actggggctg cgccatgatc
     5701 tgaaaaaagc cacgctgggg ctcggcgtat cgacgctcct tgtcgcgtcg cttctcgggc
     5761 cgctctcggc gctctttttc gtgctcggct tcggcgtgct cggagtggga ctcggttttc
     5821 tggcgaaacg ctgcgaaaag ggtgtggagg ttctcttgta cggaattctt ctctccctgg
     5881 gaagtaagct tctcctgatg gtcatcgcgg gaaaagtgac ggggatcaat ccgtttcagc
     5941 tcgacggcgc tgagatgcag tcgatgatcg acaagatttt cctgttttac gagtcgactg
     6001 gcatgagcaa ggaatcaatc gcagcggttc gagagcagtt cgccgagtct ctccgtcttc
     6061 tgcccgttat cttccctacc attctgacga tggccgccgc gcttgactgc tacttgagct
     6121 acaccatcag ttctttcgtg ctcaagcgcg ttggaggaac gcctctccct ccccttcccc
     6181 ctttctcaat gtggcgtttc ccgaagagcg ttttcggcgc ccttgtcgcg tccattctcc
     6241 tctctctctt cggctcgcaa agcggcgagt ggaactttgc tctcagggca gagaccaatt
     6301 tacgtctttt ggtgaaatta caaagagaat ttcaaggaaa cgccgtaaat tggtatttct
     6361 tctcgcagaa agctcgcttc
//
LOCUS       DAOTIL010000041         6311 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminobacteriaceae bacterium isolate AMR_MDS_3026
            NODE_14644_length_6311_cov_3.398338, whole genome shotgun sequence.
ACCESSION   DAOTIL010000041 DAOTIL010000000
VERSION     DAOTIL010000041.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868050
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminobacteriaceae bacterium (wastewater metagenome)
  ORGANISM  Aminobacteriaceae bacterium
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae.
REFERENCE   1  (bases 1 to 6311)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6311)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:19:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,224
            CDSs (total)                      :: 3,198
            Genes (coding)                    :: 3,184
            CDSs (with protein)               :: 3,184
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6311
                     /organism="Aminobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14644_length_6311_cov_3.398338"
                     /isolate="AMR_MDS_3026"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:3034609"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..523)
                     /locus_tag="P5201_01580"
     CDS             complement(<1..523)
                     /locus_tag="P5201_01580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013049251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrilase-related carbon-nitrogen hydrolase"
                     /protein_id="HRV97255.1"
                     /translation="MKLRVGVLQLDIAIGDKQRNCQNIEDWMASFYVPSELPTAVVVP
                     EMWNTGYALSRAKELADPEGAEAAAFLGELARKYGVWFVGGSVLASTKKGYANRAQAI
                     NPEGKLVAQYDKAHLIRLMEEEKYFTGGRKACLFDLEGAKAGCVICYDIRFCEWIRTY
                     ALKGAEVLFVSSQW"
     gene            834..1205
                     /locus_tag="P5201_01585"
     CDS             834..1205
                     /locus_tag="P5201_01585"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRV97256.1"
                     /translation="MAYITCEVCGTYIDHNYNTTKIIGRYNSMALLCEWHHAVVSALI
                     ARHGTVNWRALGRATSQVQEEKPNVLTEPERWLDYDSVARLKELYEEEIVRDPTDWPP
                     YESPNAKRAEEEEASDRYLYR"
     gene            1226..1513
                     /locus_tag="P5201_01590"
     CDS             1226..1513
                     /locus_tag="P5201_01590"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRV97257.1"
                     /translation="MPFRWPVFEEAVPVDSVSSWTAVQESYDQRNDDCYYIVTLLKEG
                     APVRSFMVKVDVGWAGDDWTTPEFARRLEEEIGRAARRGETNTDYPGPLAR"
     gene            1841..2878
                     /gene="trpD"
                     /locus_tag="P5201_01595"
     CDS             1841..2878
                     /gene="trpD"
                     /locus_tag="P5201_01595"
                     /EC_number="2.4.2.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005660212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004048 - anthranilate
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anthranilate phosphoribosyltransferase"
                     /protein_id="HRV97258.1"
                     /translation="MFAQTLSKVIERKNLESAEMEEAMEALVEGSVPPVQTAAFLTAL
                     RAKGETKEEIGGAVRLLLRKARRVPARRELLMDNCGTGGDCGGTFNISTAAAFVAAGG
                     GIPMAKHGNRAVSSKCGAADVAEALGAEMPDTPERAASCLDAAGLVLLFAPHFHPILR
                     NVAEIRKSLGVRTIFNILGPLLNPAPLTHQVMGVFDPGMAPLVANVLLALGRDRGMVI
                     SGHGGFDELSLSGPNRLVLFSEEGVREAVITPEEAGLARRGDDEFLGGTATENAAIIE
                     DILAGKKGGPRDVVLLNASAVFLVSGMASSWKEGVALASESIDSGRAAEVLEKIRSFS
                     QQREDVIPRSA"
     gene            2894..3652
                     /gene="trpC"
                     /locus_tag="P5201_01600"
     CDS             2894..3652
                     /gene="trpC"
                     /locus_tag="P5201_01600"
                     /EC_number="4.1.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006301398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004425 - indole-3-glycerol-phosphate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0006568 - tryptophan metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="indole-3-glycerol phosphate synthase TrpC"
                     /protein_id="HRV97259.1"
                     /translation="MILERILHQKMEETNELTAPRRKMSAALKRSGLSVIGEIKRASP
                     SKGIINGGVDPLRQLRLYEQGGADAVSILTDRVFFGGGPEDFSSLRTRTSLPLLRKDF
                     LVSEVQIYESLFLGADALLLIAAALEKERLEALLSLTYSLGMEAIVEVHTEEELLSVL
                     ETDAPILGVNNRNLNDFTVDLRTTARLMDALRSAEGEGSRIVVAESGVRSEEDAGYLA
                     ETGVDAILVGEALMRASNPEETLRSFKRKGRRAA"
     gene            3649..4284
                     /locus_tag="P5201_01605"
     CDS             3649..4284
                     /locus_tag="P5201_01605"
                     /inference="COORDINATES: protein motif:HMM:NF012900.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylanthranilate isomerase"
                     /protein_id="HRV97260.1"
                     /translation="MTFVKVCGITRREDIEAAAGLGVDALGFILVPASPRFLPAEEAA
                     ALACTVPEGIRKVAVMVNPSGTEVEAVERTGVFDCIQFHGSETPETLSGCRLETIKAF
                     SVTEEADLENAALFDAASFFLFDSKKRRYDKGVAFDHSMLRGRTFAKPFILAGGLGPD
                     TLRGALREARPFGVDLNSGVEDAPGVKNRVKLAVAVATVRDFDTSERREHR"
     gene            4281..5447
                     /gene="trpB"
                     /locus_tag="P5201_01610"
     CDS             4281..5447
                     /gene="trpB"
                     /locus_tag="P5201_01610"
                     /EC_number="4.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006582999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004834 - tryptophan synthase activity
                     [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan synthase subunit beta"
                     /protein_id="HRV97261.1"
                     /translation="MKTSMYFGIFGGQYVPETLVPALDELETAYLGTLDDASFNGELL
                     ALLHNYSGRPTPLWFAPRITESLGGAKVYLKREDLNHTGAHKINNALGQVLLASKMGK
                     KRIIAETGAGQHGVAVATAAALFGLECVVYMGEEDVHRQAPNVARMNLLGTKVVSVLS
                     GTRTLKDAVNEAIRDWVTNVEDSFYVIGSVMGPHPYPLMVRNFQKVIGEEARAQMLEC
                     EGRLPDQVIACVGGGSNAMGIFHAFVGDRDVELIGVEAAGRGVDTGLHAATISGGKPG
                     VLHGSYSYVLFDEDGQIQPAHSISAGLDYPGVGPEHSHLAATGRATYVSVTDDEAVEA
                     FHRTCRLEGIIPALESSHALAHVYRTAAALPKETIVLVNLSGRGDKDMDTILSL"
     gene            5463..6254
                     /gene="trpA"
                     /locus_tag="P5201_01615"
     CDS             5463..6254
                     /gene="trpA"
                     /locus_tag="P5201_01615"
                     /EC_number="4.2.1.20"
                     /inference="COORDINATES: protein motif:HMM:NF012511.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004834 - tryptophan synthase activity
                     [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan synthase subunit alpha"
                     /protein_id="HRV97262.1"
                     /translation="MNGIAQAFDGKKALITYITAGDPDLDTTAFLLKLLDESGVDILE
                     VGIPFSDPLADGPVIQQAGQRALKSGTTLKKLLAMLASLKGEVKAPLVLMGYMNSLIA
                     YGLETFAKDAADAGVAGVIVPDLPFHQSEELAGLLRSRGVDFILMVTPNSTPERLKAI
                     AEKASGFLYCVSLLGVTGADEKGNAHLEEYMQRVRASTKLPLALGFGVSTPQRAKVAA
                     QVADGVIVGSALISALEPCGTDRKQLVAKATEFIGSLRAGVDSAG"
BASE COUNT         1198 a         2000 c         2033 g         1080 t
ORIGIN      
        1 gccactgcga actcacgaag agcacctcgg cccccttcag cgcgtacgtg cggatccact
       61 cgcaaaagcg gatgtcgtag cagataacgc acccggcctt cgcgccttcc aggtcgaaga
      121 gacaggcctt ccggcctccc gtgaagtact tctcctcctc catcaggcgg atgaggtgcg
      181 ccttgtcgta ctgcgccacg agcttcccct ccgggttgat cgcctgcgct ctgttcgcgt
      241 accccttctt cgtcgatgcg agcaccgaac cgccgacgaa ccagacgccg tactttcggg
      301 ccagttcgcc gagaaaggcc gccgcctccg ctccttcggg gtcggccagc tccttcgccc
      361 gcgagagcgc gtaccccgtg ttccacatct cgggcacgac gacggcagtc ggcagctccg
      421 aaggaacgta gaacgaggcc atccagtcct cgatattttg gcagttccgc tgtttgtcgc
      481 cgatggcaat gtcaagctgc agcacaccca ctcgaagttt catccgctac ctcctcgatt
      541 tcggccggga acgcccctga ccgtatagta aagtcgatgc tgtccatttt tgaaaaatac
      601 tgtgaatacg gcccgatatg tttccgctct tatcctatga atagtccatg aaattcttct
      661 tgtcaagagg aaattttctg cggaggccaa aaatacgctt gtgaccggcc atttccggtg
      721 ctacactgcc attgttcggg acaaagcccg gcgcggcgcg tcgagccgcc ggactgcggc
      781 gaagcgcacc ggggcgggag tacatacacg acgtacatcc ggaggtgtaa aaaatggcgt
      841 atatcacctg cgaggtctgc ggcacgtaca tcgaccacaa ctacaacacg accaagatca
      901 tcgggcgcta caactccatg gcgctgctct gcgaatggca tcatgcagtc gtctcggcgc
      961 tgatcgcccg gcacggtacg gtcaactggc gggcgctggg ccgcgcgacc agtcaggtcc
     1021 aggaagagaa gccgaacgtg ctgacggagc cggagcgctg gctagactat gattctgttg
     1081 cgcgtctgaa ggaactctac gaggaagaga tcgtccggga tccgacggac tggcctccct
     1141 acgagtctcc gaatgcgaag cgggcggaag aggaagaggc ctccgaccgc tacctctacc
     1201 ggtgacggga atgccggggg aagagatgcc ttttcgctgg cccgtcttcg aagaagctgt
     1261 gcccgtcgat tccgtctcct cctggaccgc cgtacaggag agctatgacc agcgcaacga
     1321 cgactgctac tacatcgtta ccctcctcaa agaaggggct cccgtccgca gcttcatggt
     1381 caaggtcgat gtcggctggg caggcgacga ttggacgacg ccggagttcg cccggcggct
     1441 tgaggaagag atcggccggg cggcgcgccg gggcgagacg aacacggact acccgggccc
     1501 cctcgctcga tgaatgtttc cgcttattgc gtcgtcttct caaaagtttc gcttgacaga
     1561 tcttccgcaa ctgtgatatc ttctccgcaa tcgaaatcgg ggaggacatt ctcatgattc
     1621 gtaccacact caataagaat tggtggtggc gggcttgcta gcctgccgcc ggtgaccata
     1681 aagctcgcga tataagaaca acgcgtgttc tttaaggccg tggaggcagg tccctcaagg
     1741 gatcgtactc tacggccttt ttgtattccc agcgggggac agaggccgaa caggcttctc
     1801 tcccccgctt tctctttatc agggcaaagg aggaaatagt atgtttgcac agactctctc
     1861 gaaggtgatc gaacggaaaa atctggaaag cgcggagatg gaggaggcca tggaggctct
     1921 tgtggagggg agcgtacctc ccgtgcagac ggccgcgttt ctgacggccc tccgggcgaa
     1981 gggggagacg aaagaagaga tcggcggcgc ggtccggctg ctgctgcgga aggcgcggag
     2041 agttccggcg cggcgcgagc tgctgatgga caactgcggc accggcggcg actgcggggg
     2101 gacgttcaac atctccacgg cggcggcctt cgtggccgcg ggcggcggca ttccgatggc
     2161 gaagcacggc aatcgcgcag tctccagcaa gtgcggcgcc gcggatgtcg ccgaagcgct
     2221 gggcgcggag atgcccgaca cgcccgaacg cgccgcctcc tgcctcgacg ctgcggggct
     2281 ggtgctcctc ttcgcgccgc acttccaccc gatactgcgg aacgtggccg agatacggaa
     2341 gagcctcggc gtgcggacga tcttcaacat cctcggtccc ctgctgaatc ccgcgccgct
     2401 gacgcatcag gtgatgggcg tcttcgaccc cggcatggcc ccgttggttg cgaacgtcct
     2461 cctcgcgctg gggcgggatc gcggcatggt gatctccgga cacggcggtt tcgacgagct
     2521 ctcactcagc ggcccgaacc gcctggttct tttctcggaa gagggagtga gggaggccgt
     2581 catcactccg gaagaagcgg ggctcgcccg caggggcgac gacgaatttc tcggcggaac
     2641 ggcgacggag aacgccgcga tcatcgagga catcctcgca ggaaagaagg ggggcccccg
     2701 agacgtggtt ctcttgaacg cctcggccgt cttcctcgtc tccggcatgg cctccagctg
     2761 gaaggagggg gtcgcgctcg cctcggagag catcgactca ggccgcgccg cggaagtgct
     2821 ggaaaaaatc cgttccttct cgcagcagcg ggaggacgtt atcccccgtt cggcatgaag
     2881 ggaggccgtt gtaatgattc ttgaacgcat tctgcaccag aagatggagg agacgaacga
     2941 gctgacggcg ccgcggcgga agatgtcggc cgctctcaag cgaagcggac tctccgtgat
     3001 cggcgagatc aagcgcgcat ccccgagcaa ggggatcatc aacggcggcg tcgacccgct
     3061 gcgccagctt cgactgtacg agcagggagg cgcggacgcc gtctccatcc tcaccgaccg
     3121 ggtcttcttc ggcggcggcc cggaggattt cagctccctg aggacgcgga caagccttcc
     3181 gctgcttcgg aaggactttc tggtgagcga agtgcagatc tacgagagcc tcttcctcgg
     3241 cgcggacgcg ctgctgctca tcgccgcggc gctcgaaaaa gagcgtcttg aggcgctgct
     3301 ctccctgaca tattcgctgg ggatggaggc gatcgtcgag gtgcacaccg aggaggagct
     3361 gttgagcgtg ctggagactg acgccccgat ccttggcgtc aacaaccgga atctgaacga
     3421 cttcaccgtc gatctgcgga ccacggcgcg cctgatggac gcgctccgtt ccgcggaggg
     3481 ggaggggagc cgcatcgtgg tggcggagag cggcgtgcga agcgaagaag acgccggata
     3541 ccttgccgag accggcgtcg acgcgatcct cgtcggcgag gccctcatgc gggcatccaa
     3601 cccggaggag acgctccgct ccttcaagag aaaggggcgg cgtgcggcat gacgttcgtg
     3661 aaggtctgcg gcataacgcg gcgtgaggat atcgaagcgg ctgccgggct gggcgtcgac
     3721 gcgctgggct tcatcctcgt cccggcgagc ccgcgctttc tcccggcgga agaggccgcg
     3781 gcgctcgcat gtactgtccc ggagggcatc cgcaaggtcg ccgtgatggt gaacccgtcc
     3841 ggaacggagg tcgaggctgt ggaaagaacg ggcgtgttcg actgcatcca gttccacggc
     3901 tcggagacgc cggagacgct ttccggctgc cgtcttgaga ccatcaaggc gttctccgtg
     3961 acggaggagg ccgacctgga gaacgcggcg ctcttcgacg ccgcgtcgtt cttcctcttc
     4021 gacagcaaga agcgccgcta cgacaagggc gttgccttcg accactcgat gctgcgcgga
     4081 cggacgttcg ctaaaccctt tattctcgcg ggaggactgg gcccggacac cctgcggggc
     4141 gcgctgcggg aggcccgccc cttcggggtc gacctcaaca gcggggtcga ggacgccccc
     4201 ggcgtcaaga acagggtgaa gctcgccgtc gcagtggcga cggtccgtga tttcgacact
     4261 tccgaaagga gagagcaccg atgaaaacat ccatgtactt cggcatcttc ggagggcagt
     4321 acgtccccga aacgcttgtt ccggccctcg acgaactgga gacggcctac ctcggcacgc
     4381 tggacgacgc gtcgttcaac ggagaactgc ttgccctgct gcacaactac agcggacggc
     4441 cgacgcccct ctggttcgcg ccgcgcatca ccgagtcgct cggcggagcc aaggtctacc
     4501 tgaagcgcga ggatctgaac cacaccggcg cgcacaagat caacaacgcg ctcggccagg
     4561 tgctgctcgc ctccaaaatg gggaagaaac gcatcatcgc ggagaccggc gcggggcagc
     4621 acggggtggc ggtggctact gcggcggcgc tcttcgggct ggagtgcgtg gtgtacatgg
     4681 gggaggagga cgtccaccgg caggccccca acgtggcccg gatgaatctg ctggggacga
     4741 aggtcgtctc cgttctctcc ggaacgcgca ccctcaagga cgcggtcaac gaggccatcc
     4801 gcgactgggt gaccaacgtc gaggactcat tctacgtcat cggctcggtg atggggccgc
     4861 acccgtaccc gctgatggtc cgcaacttcc agaaggtcat cggggaggag gcgcgcgcgc
     4921 agatgctcga atgcgaggga cggctccccg accaggtgat cgcctgcgtc ggcggcggca
     4981 gcaacgcgat gggcatcttc cacgctttcg tcggcgaccg cgacgttgaa ttgatcggcg
     5041 tcgaggcggc gggcagggga gtggacacag gccttcacgc cgccaccatc tcgggcggga
     5101 agccgggcgt gctccacggc tcgtactcgt acgtcctctt cgacgaagac gggcagatac
     5161 agcctgcgca ctccatctcc gccgggctgg actaccccgg cgtcgggccg gaacacagcc
     5221 atctcgccgc tacggggcgc gcaacctacg tctccgtcac cgacgacgag gcagtggagg
     5281 ccttccaccg cacctgccgc ctcgaaggaa tcatcccggc gcttgaaagc tcgcacgcgc
     5341 tcgcgcacgt ctaccggacc gccgccgctc tcccgaagga aacgatcgtg ctggtgaacc
     5401 tctccggccg cggcgacaag gacatggaca ccatcctgtc cctttgaaag gagttacctc
     5461 taatgaacgg tatagctcag gccttcgacg gcaaaaaagc gctcatcacc tacatcacgg
     5521 cgggcgaccc cgaccttgac accacggcct tcctgctgaa gctgctcgac gaaagcggcg
     5581 tggacattct cgaagtgggc atccccttct ccgatccgct ggccgacggt cccgtcatcc
     5641 agcaggcggg gcagcgcgcg ctcaagagcg gcacgacgct caaaaaactc ctcgccatgc
     5701 tcgcgtcgct gaaaggagag gtcaaggccc cgctcgtgct catggggtac atgaattctc
     5761 tgatagccta cggtctcgaa accttcgcga aggacgccgc cgacgcgggc gtcgcgggag
     5821 tcatcgtccc cgacctcccc ttccatcaga gcgaagaact cgcggggctg cttcgttccc
     5881 gcggagtgga cttcatcctc atggtcacgc cgaacagcac ccccgaacgg ttgaaggcca
     5941 tcgcggagaa ggcgtccggc ttcctctact gcgtctcgct cctcggggtg acgggcgccg
     6001 atgagaaagg aaacgcgcac ctcgaagagt acatgcagcg cgtccgcgcc tccacgaagc
     6061 tcccgctcgc cctcggcttc ggcgtgagca cgccccaacg ggcgaaggta gcggcgcagg
     6121 tcgccgacgg cgtgatcgtc ggcagcgcgc tcatctccgc gctggagccg tgtggaaccg
     6181 acagaaaaca gctcgtggcc aaggcgacgg agttcatcgg ctccctccgc gcaggcgtcg
     6241 actcggcggg gtagagggaa gtccggcgaa agcaacctgt ttcggcaacg gacaacggaa
     6301 tacagagtga a
//
LOCUS       DAOTIL010000042         6168 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminobacteriaceae bacterium isolate AMR_MDS_3026
            NODE_15096_length_6168_cov_2.803861, whole genome shotgun sequence.
ACCESSION   DAOTIL010000042 DAOTIL010000000
VERSION     DAOTIL010000042.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868050
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminobacteriaceae bacterium (wastewater metagenome)
  ORGANISM  Aminobacteriaceae bacterium
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae.
REFERENCE   1  (bases 1 to 6168)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6168)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:19:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,224
            CDSs (total)                      :: 3,198
            Genes (coding)                    :: 3,184
            CDSs (with protein)               :: 3,184
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6168
                     /organism="Aminobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15096_length_6168_cov_2.803861"
                     /isolate="AMR_MDS_3026"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:3034609"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..242)
                     /locus_tag="P5201_01620"
     CDS             complement(<1..242)
                     /locus_tag="P5201_01620"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRV97263.1"
                     /translation="MTTHAENAPGPVFDRAGRKPVLTHLGKELLGLSEEMLRLEERVR
                     NLASGSSEPSGELNVATGESVMVSRLGEPLARFKQR"
     gene            253..1005
                     /locus_tag="P5201_01625"
     CDS             253..1005
                     /locus_tag="P5201_01625"
                     /inference="COORDINATES: protein motif:HMM:NF012544.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HRV97264.1"
                     /translation="MGVFAGVCAALALTVPSPAAFGETLVLLFWAVIGWEILGNYTLE
                     IESPKRSIPLAAGIAFCAICAVYLAIAAALPRIGAGGTIPSMTDLLTPLFGSFASPLL
                     AVFGILLCVCTYLMVVGGVSRLVASMAERGELPRMLALRNKEGAPSRAIAALGCVHGA
                     GLLLSSLSVLDVASLVGAANGLFLLNSLLAVAAAARLLRSPAVRTASAVLAACFLILF
                     ALADRTALLVSVTVLLFSLKEASRGDRVAVSG"
     gene            complement(1057..2607)
                     /locus_tag="P5201_01630"
     CDS             complement(1057..2607)
                     /locus_tag="P5201_01630"
                     /inference="COORDINATES: protein motif:HMM:NF024627.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="right-handed parallel beta-helix
                     repeat-containing protein"
                     /protein_id="HRV97265.1"
                     /translation="MERTSAERRNLFTKTGGLLAGIAAVLLMALLFAFMPGTGEAATW
                     TVTNGGDGGTGALRSVIESAAAGDSVVFDSSVSIVNLNTYLFVNKALTITGPVTIKQN
                     GAGQRVLYVTGDCTFNNLTITGGNLGDPNSNGGAGIYNYSTLTMNNCTVTGNTGGGGI
                     LNKQNLTMNSCTISGNAASGHLKAGGIASTATGSWEVSLTMTDCIVENNIGSDRANSG
                     GIYNGVDGTLVMTRSTVRNNECSSLGGGMYISAYSSTTLKNRSVVTGNRPDQICCGYG
                     ASYTTDGTCTIGGASGTSATALSGFAEGASPAPRKTTGEPDVAAVERDLRSPSSELFG
                     AVKNLLESDLGGLPGDVSAGLSSIDATLYNAFAYEDVPLADASGEGELVIEFTASWPQ
                     NVRYYAALAEWEDAAAGALAVKEYVIPERGVQFELKPGQPLPDGVTPPDFYEEGEGLM
                     TWRNVIADNGPYDHNSAEGLVSFRAASIRAEAQTASSGGSGCSAVGAPPFALLLGAPL
                     LLLRKKHP"
     gene            complement(2818..3723)
                     /gene="pgsW"
                     /locus_tag="P5201_01635"
     CDS             complement(2818..3723)
                     /gene="pgsW"
                     /locus_tag="P5201_01635"
                     /inference="COORDINATES: protein motif:HMM:TIGR04332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="poly-gamma-glutamate system protein"
                     /protein_id="HRV97266.1"
                     /translation="MKLFRTLLFFILLATAADASAIEQGSASRLMARCEGVLYAEKCR
                     LGLYEALTDPEKTGLIGVEFSPLTTSQGHLPDKQASARPAMADAVAEYLRRAGVGEGD
                     WVAVNASASFPGFILASLCAVESMRANAQIVFSYGASMYGGTDPEFTFPVMLDLLNRE
                     GLLSTRLAAVAPGGAGDRMAETLLEDPRPTVDLLLASRSEERVDAPSVAEAIRRRLEI
                     FDDTPRPVKCFISCGGPVVALGTTEAVLELPHGLITRFEPIPKDPERGLLFDFLDRGT
                     PVLHLLFVRGICEDFGIPYERVAGQ"
     gene            complement(3823..5412)
                     /locus_tag="P5201_01640"
     CDS             complement(3823..5412)
                     /locus_tag="P5201_01640"
                     /EC_number="3.4.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015556604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016805 - dipeptidase activity [Evidence
                     IEA];
                     GO_function: GO:0070004 - cysteine-type exopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="C69 family dipeptidase"
                     /protein_id="HRV97267.1"
                     /translation="MLCAMLCVILLSSAALACTVLAVGKDATVDGSAMITHNDDSRVA
                     NSRLFIIPEADWPEGSMRSVVKDAHGYEGDGQVIGEIPQVPHTFRYFFSRYSFMNEKG
                     VAISESTDSVEVTDERSKKVEQIMAKDAVGMMDAWILQDIMLERASTAREAVEIMGKL
                     VEEYGWYDAPETMPLTDGNEVWIAEFYGNKIWAAWRMPDDHIFVGANRARLRHLDLTD
                     KENVMYCPDLVEFAVKNGFIEEKDVNMKDFSPADVYCPSTALYATRREWRVLSLAAPD
                     SFKLGPDEYDYPMSIKPDKKLSVHDVLRIKGDWYEGTPFDLSKGIQAGPWGNPIRFAN
                     SSKKNPSASWERSVNMMRTAYVHIAQVRGDLPVEVRGISWYGYGAPDTTYITPLWPVM
                     SKLPPLYGIGDRYHDYDPKSGWWVNTRVQELAGLRYQDARKEIHAARDAKLNPLYVQT
                     RMIQDKAAEMLKAGDKDGAVSLITDFAYAHAVDWNQRWLALGDRLLSKYALGYTDVKT
                     TPYPEEWNEIVGFDTPKRSTK"
     gene            complement(5546..>6168)
                     /locus_tag="P5201_01645"
     CDS             complement(5546..>6168)
                     /locus_tag="P5201_01645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008521715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="deacylase"
                     /protein_id="HRV97268.1"
                     /translation="PGRPDGNLTEQLTFAIMELNRKEKITLFVDAHEASLMYPVVSTY
                     VAHDRALDMAMMASMMLSATEFPMKCEASPKNLRGLSHRDAGDFCDSLVILMETPEPF
                     IDRVAGKITEKLMMDGKDEFLQTAAEHGLLFTDYDISVGCPMWDRVGRHLSGALAMIQ
                     QMNDFLMEPEKELQLSFPNYSDLKEHGLGHFLNDPAKADPAKVFLN"
BASE COUNT          958 a         1893 c         2053 g         1264 t
ORIGIN      
        1 tatcgctgtt tgaagcgggc gagaggctct cccagccttg acaccatgac gctctccccc
       61 gtcgccacgt tcagttcgcc cgagggttcg gatgagccgg acgccaggtt cctgacgcgc
      121 tcctccaggc ggagcatctc ttcggagagc ccgagcagct ccttccccag atgcgtcagg
      181 accggctttc tgcccgctct gtcgaagacg gggcctggcg cgttttcagc gtgggtggtc
      241 acgctcgtcc ttatgggggt gttcgcaggg gtgtgcgcgg cgcttgcgct gaccgttccg
      301 tcccccgctg ccttcgggga gacgctggtg ctcctcttct gggccgtgat cgggtgggag
      361 attctgggaa actacacgct cgaaatcgag tcgcccaagc ggagcattcc cctggcggcg
      421 gggatcgcct tctgcgcgat ctgcgcagtg tatctcgcga tagccgccgc gctgccgcgc
      481 atcggggcgg gcggaaccat tccctcgatg acggatctgc tgacgcccct cttcgggagt
      541 ttcgcctctc cgctgctcgc ggtgttcggc atattgctct gcgtctgcac gtacctcatg
      601 gtggtcggag gagtctcccg ccttgtcgcg tcgatggccg agcgcggaga gcttccccgc
      661 atgctcgccc tcaggaacaa ggagggcgcg ccgtccagag cgatagcggc tcttgggtgc
      721 gtccacgggg cggggctgct gctctcctcc ctctctgtgc tggacgtcgc ttctctggtg
      781 ggcgccgcca acggactctt tctgctgaac tcgctgctgg cggtcgcggc ggcggctcgc
      841 ctgctgcgct ctccggccgt ccggacggcc tctgccgtct tggccgcgtg cttcctgatt
      901 ctcttcgctc tcgccgacag gacggcgctg ctggtctcgg tgacagtgct gctcttctct
      961 ttgaaagagg cgagccgagg cgaccgggtg gccgtctccg gatgaatgct cgaagggggc
     1021 gcgagaaaac gcttttctcc gcgccctctt cgggttttac gggtgttttt tccgtaggag
     1081 caacagcggc gcccccagca ggagcgcgaa gggcggcgcg cctacggccg agcagcccga
     1141 gcctcccgag gaggccgtct gcgcttcggc gcgtatcgag gccgcgcgga aggagacgag
     1201 gccctcggcg ctgttgtggt cgtacgggcc gttgtccgcg atcacgttgc gccaggtcat
     1261 caatccttcg ccctcctcgt agaagtcggg aggcgtcacg ccgtcgggga gcggctgacc
     1321 gggcttaagc tcgaactgga cgccgcgctc ggggatgacg tactccttca cggcgagagc
     1381 tcccgcagcg gcatcctccc actccgccag cgcggcgtag tagcggacgt tctgcggcca
     1441 cgacgcggtg aattcgatca ccagctcgcc ctctccggag gcgtccgcaa gcggcacgtc
     1501 ctcgtaggcg aaggcgttgt acagcgtcgc gtcgatgctc gaaagccccg ccgagacgtc
     1561 tccgggaagt cccccgaggt cggactccag gagattcttc acagcgccga agagttcgct
     1621 cgagggcgag cgcagatccc gctcgaccgc cgcgacgtcc ggttcgcccg tggtcttccg
     1681 aggcgcgggg gaagctccct cggcgaatcc ggagagcgcc gtcgcgctgg ttccggaagc
     1741 gcctccgatg gtgcaggtac cgtcggtggt ataggatgcg ccgtagccgc agcatatctg
     1801 atccggccga ttgccggtca cgacgctgcg gtttttcagc gtcgtcgagg aataggcgct
     1861 gatatacatc cctccgccga ggctgctgca ctcgttgttt ctgactgtgc tccgcgtcat
     1921 gacgagcgtt ccatcgacac cgttgtagat acctcccgaa tttgccctgt ccgagccgat
     1981 attgttctcc acgatacagt cggtcatcgt cagagacacc tcccaggagc cggtggcggt
     2041 gctggcgatt cccccggctt taaggtgtcc tgaagccgcg ttcccgctga tcgtacagct
     2101 gttcatggtg aggttttgtt tgttcaggat gccgccgcct ccggtgtttc cggtcacggt
     2161 gcagttgttc atcgtcagcg tcgaatagtt gtatatcccg gcgccgccgt tgctgttcgg
     2221 gtcgccgagg ttgccgccgg tgatcgtcag gttgttgaac gtgcaatcgc ccgtgacgta
     2281 gaggacgcgc tgcccggcgc cgttctgctt gatagtgacg ggaccggtga tcgtgagcgc
     2341 cttgttgacg aagaggtagg tgttcagatt gacgatggag acggacgaat cgaaaacgac
     2401 cgagtcgccg gccgcggcgc tctcgataac gctcctcaag gcgcccgttc cgccgtcgcc
     2461 cccgttcgtc accgtccatg tcgcggcctc tcccgtcccc ggcataaagg cgaacagcaa
     2521 cgccatgagg agtaccgccg cgattccggc gaggagacct cctgttttgg tgaatagatt
     2581 ccgtctttct gcgcttgtcc gttccatcgc gtcttccttc tttctcgtgg aatatactcc
     2641 gcgcggtcga tgaacgtacg tttttgcgcc gtcgtcgaca tgccgccgga atagtggtgc
     2701 gtacgcgagg tatcataaac ctttccactg tgtgagagtc aagagggaat ttcgattttt
     2761 cggagttttt tattacggta ttcttgcccg aagcgcgggg cggcgttctg aaagaggcta
     2821 ctgccccgcg acgcgctcgt acgggatgcc gaaatcctcg cagatgccgc ggacgaagag
     2881 gaggtggagc acgggggttc ctctgtcgag gaagtcgaag agcaggccgc gttcggggtc
     2941 tttcgggatc ggctcgaagc gcgttatcag gccatgaggg agttcaagta ccgcctcggt
     3001 cgttcccagg gcgacgacgg ggcccccgca gctgatgaag cacttcacgg ggcgcggcgt
     3061 gtcgtcgaag atttcgaggc ggcgacggat ggcttcggcc acgctcggcg cgtcgacgcg
     3121 ctcctcgctc ctggaggcca gcaggagatc gacggtcggg cgcgggtcct ccagcagcgt
     3181 ctccgccatt cggtctccgg ccccgccggg ggccacggcc gcgaggcgcg tcgaaagaag
     3241 cccctcccgg ttgaggaggt cgagcatgac gggaaaggtg aattcggggt cggttccgcc
     3301 gtacatggag gcgccgtagg agaagacgat ctgcgcgttc gcccgcatgc tctcgacggc
     3361 gcagagcgag gcgaggatga agccggggaa ggaggcgctc gcgttcacgg cgacccagtc
     3421 cccttccccg acgcccgccc gccggaggta ctccgcgacg gcgtccgcca tggcggggcg
     3481 ggccgacgcc tgtttgtccg gcagatgccc ctgactggtc gtaaggggcg agaactcgac
     3541 gccgatcagg ccggtttttt ccgggtccgt caacgcttcg tacaggccga gtctgcactt
     3601 ttctgcgtag aggacgcctt cgcagcgcgc catcaggcgg gacgccgacc cctgctcgat
     3661 ggcggaagcg tcggcggcgg ttgccagcag aatgaaaaac agcagcgttc ggaagagctt
     3721 catacggtcg cctccttggg cctcgcggcg gggcgccgcg aggatacgaa aagggcgcgg
     3781 cggctctcaa gccctccgcg ccctttcatc agcaggaaaa tactatttcg tggagcgctt
     3841 cggggtgtcg aagccgacga tctcgttcca ctcctcgggg tacggcgtgg tcttcacgtc
     3901 ggtgtagccg agggcgtact tgctcagcag cctgtcgccg agggcgagcc agcgctggtt
     3961 ccagtcgacg gcatgggcgt aggcgaagtc ggtgatcagg gagaccgcgc cgtccttatc
     4021 gccggccttc agcatctcgg ctgccttgtc ctggatcatc cgcgtctgca cgtagagcgg
     4081 attgagcttc gcgtcgcgcg cggcgtggat ctctttgcgg gcgtcctggt agcgaagtcc
     4141 tgcgagttcc tgcacgcggg tgttcaccca ccagccggat ttgggatcgt agtcgtggta
     4201 tctgtcgccg atgccgtaga gcgggggcag cttgctcatc acgggccaca ggggggtgat
     4261 gtaggtggtg tccggcgcgc cgtagccgta ccagctgatg ccgcgcactt ccacggggag
     4321 atcgccgcgg acctgggcga tgtggacgta ggccgtccgc atcatgttga cggagcgctc
     4381 ccagctggcg gaggggttct tcttgctgga attggcgaag cggatggggt tgccccaggg
     4441 accggcctgg atgcccttgc tcagatcgaa gggagtgccc tcgtaccagt cgcccttgat
     4501 gcgcagtacg tcatgcacgc tgagcttctt gtccggtttg atggacatgg ggtagtcgta
     4561 ttcgtcgggg ccgagcttga aggagtcggg agcggcgagc gagaggactc tccactcgcg
     4621 gcgtgtcgcg tagagcgcgg tgctcgggca gtagacgtcg gccgggctga agtccttcat
     4681 attgacgtcc ttttcctcga tgaatccgtt cttcacggcg aactcgacga ggtcggggca
     4741 gtacatgacg ttctccttgt cggtcaggtc gaggtggcgg agacgcgcgc ggttggctcc
     4801 cacgaagatg tggtcgtcgg gcatgcgcca tgcggcccag atcttgttgc cgtagaactc
     4861 ggcgatccag acttcgttgc cgtcggtcag gggcatcgtc tcgggagcgt cgtaccagcc
     4921 gtactcctcg acgagtttgc ccatgatctc gaccgcttca cgggccgtgc tcgcgcgctc
     4981 gagcatgatg tcctgaagga tccaggcgtc catcattccc acggcgtcct tcgccatgat
     5041 ctgttcgacc ttcttgctcc gctcgtcggt gacctcgacg gagtcggtcg attcgctgat
     5101 ggcgacgccc ttctcgttca tgaaggagta gcgggagaag aagtaccgga aggtgtgcgg
     5161 gacctgcggg atctcgccga tgacctgtcc gtcgccctcg tagccgtggg cgtccttcac
     5221 gacgctccgc atcgaacctt ccggccagtc ggcctcggga atgatgaaga ggcggctgtt
     5281 ggcgaccctg gagtcgtcgt tgtgcgtgat catggcgctt ccgtccacgg tggcgtcttt
     5341 acccaccgca agaacggtgc aggcaagggc ggccgaggag agaaggatga cgcacagcat
     5401 ggcgcacagc atggttctga atgatttcaa cacgtgtttt tcctcctgtc tgaaataaaa
     5461 ataaatgatg cataccaaag ccttcgggcg cgcggtgcac cggcgggaac gctcccgccg
     5521 ggagaagcct tacgggatag tgccgtcagt tcaaaaagac cttcgccgga tccgctttcg
     5581 caggatcgtt caggaaatgg ccgagtccgt gttccttcaa gtcggagtag ttcggaaagg
     5641 agagctgcaa ctccttctcc ggctccatca ggaagtcgtt catctgctgg atcattgcca
     5701 acgctccgga gagatggcgc cctaccctgt cccacatggg gcagccgacg gagatgtcgt
     5761 agtcggtgaa cagcaggccg tgctccgccg cggtctggag gaactcgtcc tttccgtcca
     5821 tcatcagctt ttcggtgatt ttgcccgcca ctctgtcgat gaacggttcg ggcgtctcca
     5881 tcaggatgac cagcgagtcg cagaaatcgc ccgcgtcgcg gtggctcaac ccccgcaggt
     5941 tcttggggga ggcctcgcac ttcatcggga actcggtggc cgacagcatc atggacgcca
     6001 tcatcgccat gtcgagcgcc cggtcgtggg ccacgtaggt ggagaccacg gggtacatga
     6061 gggaagcctc gtgcgcgtcg acgaagaggg tgatcttctc tttccggttc agctccatga
     6121 tggcgaaggt gagctgctcc gtcagattgc cgtccggccg gccggggt
//
LOCUS       DAOTIL010000043         6073 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminobacteriaceae bacterium isolate AMR_MDS_3026
            NODE_15407_length_6073_cov_3.987039, whole genome shotgun sequence.
ACCESSION   DAOTIL010000043 DAOTIL010000000
VERSION     DAOTIL010000043.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868050
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminobacteriaceae bacterium (wastewater metagenome)
  ORGANISM  Aminobacteriaceae bacterium
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae.
REFERENCE   1  (bases 1 to 6073)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6073)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:19:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,224
            CDSs (total)                      :: 3,198
            Genes (coding)                    :: 3,184
            CDSs (with protein)               :: 3,184
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6073
                     /organism="Aminobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15407_length_6073_cov_3.987039"
                     /isolate="AMR_MDS_3026"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:3034609"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..257)
                     /locus_tag="P5201_01650"
     CDS             complement(<1..257)
                     /locus_tag="P5201_01650"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRV97269.1"
                     /translation="MNSLLITGGTLWTGRTFVRGYALHIEGERIAAVGPEEEVRAGAP
                     SECRELRLSGEAVLPGFTDAHLHLTTWAKQKTLLDLGGATSL"
     gene            389..1297
                     /locus_tag="P5201_01655"
     CDS             389..1297
                     /locus_tag="P5201_01655"
                     /inference="COORDINATES: protein motif:HMM:NF013465.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter substrate-binding protein"
                     /protein_id="HRV97270.1"
                     /translation="MKRVLFSALAVLLLFASAAAAGPVSVFVSILPQKYFVEQIGGER
                     VDVSAMVEKGRDPHVFEPLPAQMEGLSKAKAYFAVGLPFEESLLPRAASLNPGLKIYR
                     IDEGIDKIAGGREHHEEHEDEPEQNDGKHDHHVHAPHREGADPHVWNSPREAILMAGK
                     ILEGLIEIDPDGREEYARRHADFVERLKTLDAEFAGMFRGRQGATFLVFHPAWAYFAR
                     NYGLVEVAIETEGKEPKAADLANIISEARERGVKAIFLSPQFSRRSAEIVAQAIGASV
                     TTADPLAAEWEENLRAVAKAIAEAAK"
     gene            1451..4762
                     /locus_tag="P5201_01660"
     CDS             1451..4762
                     /locus_tag="P5201_01660"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HRV97271.1"
                     /translation="MNDRPQRAPIRGITRKRILPAGWRRRSFFRTIGFCVLLFWLPLL
                     APGIAECADRNAKAHILILHSYHDALPWSKGLNRGIGNAFAHAPFPIELYVEYLDAIH
                     HPGEDILDETTALFKKRYEDIPLDAVLLTDDPAWTFLVERRDLLFPGVPLFFCALNDF
                     HPERLEGISAVTGIAENPDMRGTVELVRKLHPGIREIPVVADRNPGSLLVLKNLERIS
                     RELDGEVTFRALTDAGFPELLEELKRLPEGTPVIFHAFLRDASGATFPSNLTVLGELS
                     ETISSPFYTFKRIDIGHGAVGGAVVSEELLGSLTARMLVDHLNGVPLEELPPVFDTPH
                     AYIFDEAQLERFGIDEQLLPQGSVVVNRHLSLYETHKKAIWGVAAVVGILLGLVVLLA
                     LNVLRRLRVEKMIVSINRNLEERVRDRTQELDRALLRAESANIAKSEFLANMSHEIRT
                     PMNGVIGMTDLLLETPLTLEQRRYALMLQQSAESLLELLNDILDFSKIEAGKLDLELI
                     DFDLHDLLGDIASAQAVRASEKGVEVLCAIDPDVPSSLFGDPGRLRQIVNNLAGNAVK
                     FTAEGEVEIGVSLTSEDEESATLAFRVRDTGIGIPEEKRDMLFKKFSQLDSSTTREYG
                     GSGLGLAISKQLVGMMGGEIGVESPAPSAGETSGGPGSLFHFTVRLKKSTATRTTARP
                     PAELQDIRILVVDDNATNREILAAYIAAQGIRSAEAANGADALRLLRRGAEEGDPFHV
                     AVIDMHMPLMDGETLGRTIKADPQLAKTCMIMLTSLGMRGDARRFADAGYSAYLTKPV
                     RCRELMGVVVPALRERYWSETESAPLLTRHTVRKQLEPCDTKVRVLLAEDNVTNQQVA
                     VGILEKFGIEVEIARNGEEAVEAVRRRSFDLIFMDIQMPLMDGLEATKRIRGMHAASG
                     EPAVPIVAMTAHAMEGDRERCIEAGMNDYLSKPVKPAAMFAMLRKQLPAKDLLPCTYT
                     ASSAVDDKKVDADVSVWNRTPFYDRLLEDRELAHRILEDFLIDIPSQIRQLRATLKKG
                     DSEGARRRAHAIKGAAASVNGEPLRLAALELERRALAGEQQELAFLADRLEASFEDLR
                     AAMLKELRNRTKSHP"
     gene            4982..5518
                     /locus_tag="P5201_01665"
     CDS             4982..5518
                     /locus_tag="P5201_01665"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing phosphohydrolase"
                     /protein_id="HRV97272.1"
                     /translation="MPKNTLLASDALIYKDLVESSPDLICRFLPDTTVTFANEALCRA
                     LEIDRSELLGRHFSELIPPEEVQPAREKVGSLSADGHADILQRRHERLDGSGYPQGLK
                     NEEIPLEARIVAVADTVEAMASHRPYRPSLGVDTALRFVEEGKDRLFDRNVVDACSEL
                     FAEGYSFESDGAAPALLH"
     gene            5548..>6073
                     /locus_tag="P5201_01670"
     CDS             5548..>6073
                     /locus_tag="P5201_01670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005660888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HRV97273.1"
                     /translation="MNQWIAFWNILLKDMRTYYMKPPNISWGLLFPLAWTGMFFIRSG
                     SGLESIPALLPGVTALSILFGSTSVLAVTVTFEKKNRSFERLLLAPISFELLMLAKTS
                     GAILFGVLNAFVPVLMASFIADLSPIDWRAFVPAVVLLSTASTFLGLFIAAAAGEIFE
                     AQTFSNFFRFPMIFL"
BASE COUNT         1268 a         1815 c         1831 g         1159 t
ORIGIN      
        1 agcgacgtcg cccccccgag atcgagcagc gtcttctgtt tggcccaggt ggtcagatgg
       61 aggtgcgcgt cggtgaagcc gggaagcacc gcctcacccg acagacgaag ctcgcggcac
      121 tccgacggcg cgcccgcgcg gacctcctcc tccggaccga ccgcggcgat tcgttctcct
      181 tctatatgca gcgcgtatcc ccggacgaat gttcttcccg tccagagggt tcctccggtg
      241 atgagcagac tgttcatgga cgccctcctt acacaaaaga ttccggtgtt attatagcgg
      301 atacgaacag cgcggcgcac gctgaaagca tgtggagaaa ggggcaatcg tgatagaatc
      361 atgccgcaat cttcgggagg tgttttttat gaaaagagtt cttttttcag cgctggcagt
      421 gctgctgctt tttgcgtcgg ccgctgcggc ggggccggtt tccgtcttcg tctccattct
      481 gccgcagaag tacttcgtcg agcagatcgg cggcgagcgc gtcgacgtct cggcaatggt
      541 ggagaagggg cgcgatccgc acgttttcga gccgcttccc gcgcagatgg aggggctgtc
      601 gaaggcgaag gcgtacttcg cggtcgggct tcccttcgag gaaagcctgc ttcccagggc
      661 ggcgagcctg aaccccgggc tgaagatcta ccgcatcgac gaggggatcg acaagatcgc
      721 gggcggccgc gaacatcatg aggaacatga agacgagccg gaacagaatg acggaaagca
      781 cgatcaccac gtacatgccc cccaccgcga gggcgccgac ccgcacgtct ggaacagccc
      841 ccgtgaagcg atcctcatgg cggggaagat cctcgaagga ctgatcgaga tcgaccccga
      901 cggcagggag gagtatgcgc gacgccacgc cgacttcgtg gagcgcctta aaacgctgga
      961 cgccgaattc gccgggatgt tccgcggcag gcagggagcg acctttctcg tcttccaccc
     1021 cgcatgggct tacttcgcca gaaactacgg ccttgtcgag gtcgccatcg aaacggaggg
     1081 caaggaaccc aaggcagccg atctggcgaa catcatctcg gaggccaggg aacggggcgt
     1141 caaggcgata ttcctctcgc cgcagttctc gagaagaagc gcggaaatcg tcgcgcaggc
     1201 catcggcgca tccgtcacaa ccgccgaccc gctcgccgcg gagtgggagg aaaacctccg
     1261 agcagtggcg aaggcaatag cggaggcggc gaaataacaa cactacgctc gtttcgaata
     1321 cgcacagccg gagggggact cggcttccct tccggctgtg cgtattcggg acttttacaa
     1381 attgactgaa tggattttta cccgtatgat ttcattattc ttcgtcttgc gcagttgaaa
     1441 ggagcgctcc atgaacgaca ggccgcaaag agcccccatc cgcgggatca cccgtaaaag
     1501 aatacttccg gcgggctgga ggcgccgttc ttttttccgg acgataggtt tctgcgttct
     1561 ccttttctgg ctgccgcttc ttgctccggg aatcgccgag tgcgcggacc ggaacgccaa
     1621 agcgcacatc ctgatcctcc attcgtacca tgatgcgctg ccctggagca aaggactgaa
     1681 ccgcggcatt ggaaacgcct tcgcgcacgc accgtttccg atagagttat acgtggaata
     1741 tctggacgcc attcaccacc cgggagagga tatcctcgac gaaacgacgg cgctgttcaa
     1801 aaagaggtac gaggatattc ctcttgacgc cgtcctgctc accgacgacc cagcgtggac
     1861 cttcctggtg gagcgccgcg atcttctctt tcccggagta cctctctttt tctgcgccct
     1921 caacgacttt cacccggaac gcctcgaagg aataagcgcc gtgacgggca tcgccgaaaa
     1981 cccggacatg cgcgggactg tggagctggt ccggaagctg caccccggaa tcagggagat
     2041 tcccgtcgtc gccgaccgca accccggaag tcttctcgtc ctgaagaatc tggagcgtat
     2101 ctctcgcgag ctggacggcg aagtgacgtt ccgcgcactg accgacgccg gttttcccga
     2161 gcttctggag gagctgaaac gccttccgga gggaacgccc gtgatattcc acgcattcct
     2221 gcgggacgct tcgggcgcca cgtttcccag taatctgacg gtactggggg agctctccga
     2281 aaccatctcc tctccgttct atacgttcaa gcggatcgac atcgggcacg gagcagtagg
     2341 aggagccgtc gtgagcgagg aacttctggg ctccctgaca gcccggatgc tggtcgacca
     2401 tctgaacggc gtccccttgg aggagctgcc tcccgtattc gacacccccc acgcctacat
     2461 tttcgacgag gcgcagctgg aacgcttcgg catcgacgaa cagcttctgc cgcaagggag
     2521 cgtggtcgtc aatcgtcatc tctccctcta cgagacgcat aagaaagcga tatggggcgt
     2581 ggccgcagtg gtgggaatcc tgctggggct cgtcgtgctg ctggcgctca acgtcctgcg
     2641 gcgtctgcgc gtcgagaaga tgatcgtctc catcaacagg aatctggaag agagagtccg
     2701 cgaccggacg caggaactcg accgcgccct tctgagagcg gagagcgcca acatcgcgaa
     2761 gagcgaattc cttgccaaca tgagccacga gatacgaacg ccgatgaacg gcgtcatcgg
     2821 catgaccgat ctgcttctgg agactccgct gacgctggaa cagcgccgct acgccctcat
     2881 gctgcaacag agcgcggagt cgcttctgga gctgctgaac gatattctgg atttttcgaa
     2941 gatcgaagcg ggaaaactcg atctggagct gatcgacttc gatctgcacg atcttctggg
     3001 cgatatcgcc tccgcccagg ctgtgcgcgc atcggaaaaa ggggtggaag tgctctgcgc
     3061 gatcgacccc gacgttccat cctcgctctt cggcgacccg ggacgtctcc ggcagatagt
     3121 gaacaacctc gcggggaatg cggtgaagtt caccgccgaa ggcgaggtcg aaatcggcgt
     3181 gtcgctgacg tccgaggacg aagagtccgc gacgctcgcg ttccgcgtcc gggatacggg
     3241 catcggcatc ccggaggaga agcgggacat gctcttcaag aagttcagcc agctcgactc
     3301 gtcgaccacc cgggagtacg gaggcagcgg actcggactt gccatatcga agcaactggt
     3361 cgggatgatg ggcggcgaga tcggcgtcga aagtccggcc ccctcggcag gagaaacctc
     3421 cggaggcccc ggttctctct ttcatttcac cgtacgcctc aaaaaatcaa ccgcgacgcg
     3481 cacaacggca cgtcctccgg cggagctgca ggatatcagg atcctggttg tcgacgacaa
     3541 tgcgacgaac cgcgagatcc tggccgcata catcgccgcc caagggatac gctccgccga
     3601 ggcggcgaac ggcgccgacg cgcttcgtct gcttcgccgg ggagcggagg aaggggatcc
     3661 gttccacgtc gccgtcatcg atatgcacat gcccctgatg gacggagaga cgctcggccg
     3721 gacgatcaaa gcggacccgc agcttgccaa aacgtgcatg atcatgctca cctcgctggg
     3781 aatgagaggc gacgcacgcc gcttcgcgga cgcagggtac tccgcgtacc tgacgaagcc
     3841 ggtgcgttgc agagagctta tgggggtcgt tgttccggcg ctccgggaaa gatactggag
     3901 cgagacggag agcgcgcctc ttctgacgcg ccataccgtc cgaaagcagt tggagccctg
     3961 cgatacgaaa gtacgtgttt tgctcgccga agataatgtg acaaaccagc aggtggcggt
     4021 gggtattctg gagaagttcg gcatagaggt cgagatagcc cggaacggcg aggaggctgt
     4081 cgaggctgtc cggcgccgca gcttcgacct gatcttcatg gacattcaga tgccgctcat
     4141 ggacgggctc gaagccacaa aaagaatccg cggtatgcat gccgcctcgg gagagcccgc
     4201 cgtcccgatc gtcgcgatga cagcgcacgc gatggaagga gaccgggagc gctgcatcga
     4261 agcggggatg aacgactatc tttccaaacc tgtaaaaccg gcggcgatgt tcgctatgct
     4321 gcgaaagcag ctccctgcga aggatctcct cccctgcacg tacaccgcct cttccgctgt
     4381 tgacgataag aaagtcgacg ccgatgtatc cgtctggaac cggacgccgt tttacgaccg
     4441 tctgctggag gacagagagc tcgcgcacag aattctggag gattttttaa tcgatattcc
     4501 gagccagatc cgccagctgc gcgcaacgct gaaaaaaggc gattccgaag gggcgcgcag
     4561 aagagcgcac gcaatcaagg gcgccgccgc aagcgtcaac ggagaacccc tacggctggc
     4621 cgctctggaa ctggaacgcc gcgcgctcgc cggagaacag caggaactcg catttctggc
     4681 ggaccgtctc gaagccagtt ttgaagattt gcgggcagcg atgctgaagg agttgcggaa
     4741 cagaacaaaa tcgcatccat agcactatcc aaatgaagga ctcactcttt ttacttctag
     4801 gttcagggaa gttgaacgaa agaacataaa gacatattgt gaaaatacat taaatgtgat
     4861 agaaatgtca tacaaggtat gagccgacga ccggaggcgt tctggaacgc ctcgtttttg
     4921 tttttgaaaa agggagaaca gtatccggag ctcaaaccga actcgacgcg gaggtttcac
     4981 aatgcccaaa aacactcttc ttgcttccga tgctctcatc tataaagatc tcgtcgagtc
     5041 gagtccggac cttatctgcc gttttctgcc cgatacaacc gtcaccttcg ccaacgaggc
     5101 gctctgccgg gcgcttgaaa tcgaccggag cgaactgctt ggccgtcact tttcggagtt
     5161 gatccctccg gaggaagtgc agcccgcccg tgaaaaagtg ggctccctct ccgccgacgg
     5221 acacgccgat atcttgcagc ggcgccatga acggctggac ggctccgggt atccgcaagg
     5281 actgaagaac gaggaaattc cccttgaagc gaggatcgtc gccgtggccg acactgtgga
     5341 agcgatggcg tcgcaccgcc cctaccgccc gtcgcttggc gtcgacacag cacttcgttt
     5401 cgtcgaggag gggaaggacc gtctctttga ccgaaatgtc gtcgatgcct gcagcgagct
     5461 cttcgccgaa gggtattcgt tcgaaagcga cggcgccgca cccgcgctcc tgcactaacg
     5521 agagacctgc aaaaggggga aatagccgtg aaccagtgga tcgcgttctg gaacatcctg
     5581 ctcaaggata tgcgcacgta ctacatgaag ccgcccaaca tcagctgggg gctgctcttt
     5641 ccactcgcct ggaccggcat gttcttcatc aggtcgggga gcggcctgga gagtattccc
     5701 gcgctgcttc cgggggtgac ggcactctcc atcctcttcg gctccacctc ggtgctcgcg
     5761 gtgacggtga cgttcgagaa gaagaaccgc tcgttcgaac ggctgctgct tgcgcccata
     5821 tccttcgaac tgctgatgct ggcgaagacg tccggggcca ttctcttcgg cgtgctgaac
     5881 gccttcgtcc cggtgctcat ggcgagcttc atcgcggatt tatcgccgat cgactggaga
     5941 gcgttcgttc cggcggtcgt cctgctgtcg accgcgtcga cgtttttggg gctcttcatc
     6001 gcggcggccg ccggcgagat cttcgaggcg cagacgtttt ccaacttctt ccgcttcccg
     6061 atgatcttcc tct
//
LOCUS       DAOTIL010000044         6057 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminobacteriaceae bacterium isolate AMR_MDS_3026
            NODE_15459_length_6057_cov_4.118960, whole genome shotgun sequence.
ACCESSION   DAOTIL010000044 DAOTIL010000000
VERSION     DAOTIL010000044.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868050
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminobacteriaceae bacterium (wastewater metagenome)
  ORGANISM  Aminobacteriaceae bacterium
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae.
REFERENCE   1  (bases 1 to 6057)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6057)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:19:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,224
            CDSs (total)                      :: 3,198
            Genes (coding)                    :: 3,184
            CDSs (with protein)               :: 3,184
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6057
                     /organism="Aminobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15459_length_6057_cov_4.118960"
                     /isolate="AMR_MDS_3026"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:3034609"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            246..2090
                     /locus_tag="P5201_01675"
     CDS             246..2090
                     /locus_tag="P5201_01675"
                     /inference="COORDINATES: protein motif:HMM:NF012503.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016831 - carboxy-lyase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0019752 - carboxylic acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal-dependent decarboxylase"
                     /protein_id="HRV97274.1"
                     /translation="MTEPINFNALFLGPKAENYDFFKQMLVYLMDDHAEWRRFFHPED
                     PLVVTEEEKARPDFARTLQNTREALIELAGNLQVSSNPWFSPRYLGHMNADTLIAANL
                     GYMLTLLYNPNNCAYEGSPATTALEIETGRQLAALMGYDSLTAWGHITSGGTVANYEG
                     VWLARNIASIPHAIRDCMPELVEGLDDWRLMNLPVKDVLDLVDRARERGRFDEVRDRS
                     VRGTGMAGRDLGVLLVPESKHYSWAKAADIFGIGRENLIPVPVRGDYRMDVRALESII
                     DSLAAQKRPVLAVVAVVGSTEEGAVDEVHEIVRLRERYAEKGVFFYLHVDAAYGGYAR
                     ALFLDEENRFMEYDEIKKTHADVGHGGWLVPEVYEAFKAMSSADSITVDPHKLGYIPY
                     SAGAIVAADRRVIDLISYFAAYVFEKGDDNPMLLGSYILEGSKPGAAVAAVWMAHRVV
                     PLNANGYGRIIESSIEGAFRFHQSILDSTPLEVAGRRFEALPLTRPDLNIVVYAFHEI
                     GNGSLEEMNRLNQAIYEQCSYKSGPLYISDFITSKTSLTEEEYGDAPRSFVERFGIEE
                     REWRSCGSVYVLRSCILTPYLVNNTTYEIYWGNFMKSMERAIRRLYES"
     gene            complement(2087..4111)
                     /locus_tag="P5201_01680"
     CDS             complement(2087..4111)
                     /locus_tag="P5201_01680"
                     /inference="COORDINATES: protein motif:HMM:NF018435.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp70 family protein"
                     /protein_id="HRV97275.1"
                     /translation="MITKPVLGVDFGTTKTYLSKCPGDDPSPTSVDFGDGRDGVPSAI
                     LYRKEKEPLIGQSALDEYGEATAWERASFRLVTNFKADLARSEEARAAASDFLSALLA
                     DARKQRLDVEPATRRVLFGIPSEADDAFRAALEATAERAGFGAPFLVDEPKGALLYHM
                     KQRDISVDKALGGVLVADFGGGTCDFAFLEGGRVVRSWGDMLLGGRLFDDLFFQWLCD
                     QNPGLDTELEAQGSAYFVQSHLCREVKEFFSRSMARDRGEPVSKVLAGYGRLTDLTWE
                     EFLRRGASYTPSRPFIEFSKGILPELRGRARSTAWNFLAHNGGEPLQGQKLDLFGWFR
                     ECLRSGLEEGKIERERISLVILAGGSSLWPFVGEILREEIPLREERALVRSDRPYAVI
                     AQGISLLPALKKRFARARQSLQNELPDFLENRLGALTANRVAALEEKMASLAVDIFFR
                     KEVVPLLLEFRKQGGSLALLRKRIDERTAAAEPLAREHLARLAPRFLEGLREEVRDLL
                     AEWFASHGVALPRKDLAFVLPDELSEPMLRVRTPAADGMLDVVGTAVGGLLTAGAAML
                     CGGSSTALITAGPIGLAAGAILGASAGWMLLRYGKARMKETAERMKLPALLAAFMIDE
                     KRIADLENDLSDALADHLERRFVPLKRELDDRIRTLVRDEIRALNEINSF"
     gene            complement(4108..5157)
                     /locus_tag="P5201_01685"
     CDS             complement(4108..5157)
                     /locus_tag="P5201_01685"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRV97276.1"
                     /translation="MITTKEEEEIRRRAVEKGVNVYFAYLPPNGVPEQRALESAVRRA
                     EAAASEELLPALLDSVADEPLPPERRSLLLLRMEERLPALILPEPELLSEIPPLRAAA
                     ASAFGTIAGMAFLPSLTWILLGNRAAGVFIGAPLGALFGTLAALLLPRSRKLLLLLAA
                     ILGLATVREVIRFLSGGAIFFKGWSLLREKSGSLKGLVLFPLLLATLLLLGARETRFD
                     RKRHEESVRRAFASWTEGAARVALLAASPSAPLQKDEEEAIPLLASRILSLEGACCED
                     LTAGVSELAAEVRNLGYSDENPAVLFRWNEEAAGKYTSFGAVEPGDAVRVEREPLFRN
                     GELLAKGLVRKVRRS"
     gene            5340..>6057
                     /locus_tag="P5201_01690"
     CDS             5340..>6057
                     /locus_tag="P5201_01690"
                     /inference="COORDINATES: protein motif:HMM:NF012955.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HRV97277.1"
                     /translation="MRTETLRRGTRFSVILLLCAAALLSAFGQAASASALTVRVLDVG
                     QGDSIFVVFPEGGTMLVDAGTAEAGPRVVETLRSLDAEKIDILVATHPHSDHIGGMLA
                     VLDAFPVGKVWDSGYVHGTRTQQLFLQTVLDRKIRFGRPKAGFSEEFQGVLIEVLAPV
                     KLIYGTDADVNNNSIVLHLSFGEVSFLLAADMETEQRRTVRRFPETTVLKVAHHGSHN
                     GTDARFLAMLRPKTAIISCGK"
BASE COUNT         1129 a         1914 c         1960 g         1054 t
ORIGIN      
        1 gatctcaggt tgttgcttct attcctgctg aaagaaccgc gtctatgata cccgaaaagg
       61 ccgtcaggtc aaacgaagaa acgattgtcc gaaatgaagc ggggtgtggg gcgcgcgctc
      121 ttgactttga cacggcgtta cggtgtatca tctgggtacg gaacggagac gagcctgcac
      181 ctggctggac gaatgagccg gcgagtacag gcgcgtacct cccccgcgga aaggatggcg
      241 aatcgatgac cgagcctatc aatttcaatg cgctgtttct ggggcccaag gccgaaaact
      301 acgacttctt caaacagatg ctcgtctacc tgatggacga tcatgcggag tggcggcgtt
      361 ttttccaccc cgaggatccc cttgtggtca cggaagaaga aaaggcgcgg ccggacttcg
      421 cgaggacgct ccagaatacg cgagaggcgc tcatcgagct ggcgggaaac cttcaggtca
      481 gctccaaccc gtggttttcg ccgcgctatc tggggcatat gaacgccgac acgctcattg
      541 cggccaacct tgggtacatg ctcacgcttc tgtacaaccc gaacaactgc gcctacgagg
      601 gctccccggc gacgaccgcg ctggagatcg aaacggggcg tcagctcgcc gcgctgatgg
      661 ggtacgattc gctgacggcg tgggggcata tcacctcggg cggcacggtc gccaactacg
      721 aaggggtctg gctggcgcgc aatatcgcct ccattccgca cgcgatccgc gactgcatgc
      781 ctgagcttgt cgagggcctg gacgattggc gcctcatgaa cctgcccgtg aaggacgtgc
      841 tcgacctggt ggacagggcc agggagcgcg gccgcttcga cgaggtgcgc gacaggtccg
      901 tccgggggac ggggatggcc gggcgcgacc tcggcgttct gctggttccc gagtcgaagc
      961 actactcgtg ggccaaggcg gcggatatct tcggcatcgg ccgggagaac ctgattcccg
     1021 ttccggtacg cggcgactac cggatggatg tccgcgccct cgaatccatc atcgactcac
     1081 tggccgcgca aaaacgcccc gtcctcgccg tggtggcggt cgtcggcagc accgaggagg
     1141 gagcggtgga cgaggtgcac gaaatcgtcc ggctgcgcga acggtacgcc gaaaaaggcg
     1201 ttttcttcta cctgcacgtg gacgccgcat acggcggcta cgcgcgggcg ctctttctcg
     1261 acgaggagaa tcggtttatg gagtacgacg agataaagaa gacgcatgcg gacgtggggc
     1321 acggaggctg gctggttccg gaagtgtacg aggcgttcaa ggcaatgagc agcgccgact
     1381 cgatcacggt cgatccgcac aaactgggct acatcccgta ctccgcgggc gcgatcgtcg
     1441 ccgccgaccg gcgggttatc gacctgatct cctacttcgc cgcatacgtc ttcgagaagg
     1501 gggacgacaa cccgatgctc ctagggagct atatcctgga ggggtccaag ccgggcgcgg
     1561 cggtggcggc ggtctggatg gcgcaccggg tggtgcctct gaacgcgaac ggttacggga
     1621 ggattatcga gagcagcatc gagggcgcct tccgcttcca tcaatcaatt ctcgacagca
     1681 cgccgctgga ggtcgccggc cgtcgtttcg aggcgcttcc gctgacccgt cccgatctca
     1741 acatcgtcgt ctacgccttc catgagatcg gcaacggcag cctggaggag atgaatcgtc
     1801 tgaaccaggc gatctacgag cagtgctcct acaaatcggg accgctctac atcagcgact
     1861 tcatcacgtc gaagacctcg ctgacggaag aagagtacgg ggacgcgccg cgctccttcg
     1921 tggagcgctt cggcatcgag gagagggagt ggaggagctg cggaagcgtc tacgttctcc
     1981 gctcgtgcat cctcaccccc tacctggtga acaacaccac ctacgagatt tactggggga
     2041 actttatgaa gtcgatggag cgggcgatac ggcggctcta cgagagctaa aagctgttga
     2101 tctcgttgag cgccctgatc tcgtcccgca cgagcgtccg aatgcgatcg tcgagctccc
     2161 gtttcagcgg gacgaagcgg cgctccaggt gatcggccag cgcgtcggac aggtcattct
     2221 ccagatccgc gatgcgcttt tcgtcgatca tgaaggcagc aaggagagcc ggcagcttca
     2281 tccgttccgc cgtctccttc atcctcgcct tgccgtagcg aagcagcatc caccctgcgg
     2341 aggctcccag aattgcgccc gccgcgaggc cgataggtcc cgccgtgatg agcgcggtgc
     2401 tcgatcctcc gcagagcatc gccgcgcccg ccgtcagcag tccgccgacg gccgttccga
     2461 cgacgtcgag cataccgtcc gccgcgggag tccgcacccg gagcataggc tccgagagtt
     2521 cgtccgggag aacgaacgcg aggtctttcc gcggaagggc gacgccgtgc gacgcgaacc
     2581 attccgcgag caggtcgcgt acctcctccc gaagcccttc aagaaagcgc ggagcgagcc
     2641 gggcgagatg ttcgcgggcg agaggctcgg cggcggcggt ccgctcgtcg atgcgcttcc
     2701 ggaggagtgc cagcgagccg ccctgcttcc ggaactccag cagaaggggg acgacctcct
     2761 tgcggaagaa gatgtcgacg gcgagcgagg ccatcttctc ctccagcgct gcaacgcggt
     2821 tcgccgtcag cgcgcccagg cggttctcca ggaagtcggg gagttcgttc tggagcgatt
     2881 ggcgcgcgcg ggcgaagcgc tttttgagcg cgggaagcag cgaaattccc tgggcgatca
     2941 cggcgtacgg ccggtcgctg cgcaccaggg cgcgctcctc ccgcaggggg atttcttcgc
     3001 gcaggatctc tccgacgaac ggccagaggc tgcttccgcc ggccagaatg accagcgaaa
     3061 ttcgctcgcg ttctatcttc ccctcttcaa ggccggagcg gaggcactcg cggaaccagc
     3121 cgaagaggtc gagtttctgc ccttggagcg gctcccctcc gttgtgcgcg aggaagttcc
     3181 acgccgtcga acgggcgcgc ccgcgcagct cggggagaat ccccttggag aattcgatga
     3241 aggggcgcga tggggtgtag gaggcgcctc tgcgaagaaa ctcctcccag gtcaggtcgg
     3301 tcagcctgcc gtacccggcg agcactttcg ataccggctc gccccggtcg cgcgccatcg
     3361 agcgcgaaaa gaactccttg acctcccggc agaggtggga ctggacgaag taggccgatc
     3421 cctgcgcctc cagttcggtg tcgagccccg gattctggtc gcacagccac tggaagaaga
     3481 gatcgtcgaa gagccgtccg ccgagcagca tatcccccca cgaccggacg acgcgccccc
     3541 cctcgaggaa ggcgaagtcg cacgtcccgc ccccgaagtc cgcgacgagc acgccgccga
     3601 gcgccttgtc caccgaaatg tcgcgctgct tcatgtggta gagcagcgct cccttcggtt
     3661 cgtcgacgag aaacggcgcg ccgaaacccg ctctttcggc ggttgcctcc agagcggccc
     3721 ggaatgcgtc gtccgcttcg ctgggaatgc cgaagaggac gcgccgcgtc gccggctcca
     3781 catcaagccg ctgcttccgc gcgtcggcga ggagcgcgga gaggaagtcg cttgccgctg
     3841 cgcgagcctc ctcggaacgt gcaagatccg ccttgaagtt ggtgacaagg cggaacgagg
     3901 cgcgctccca tgcggtcgcc tcgccgtact cgtcgagcgc gctctggccg ataagcggtt
     3961 ccttctcctt ccggtagagg atggcggaag gaacgccgtc ccttccgtcg ccgaaatcca
     4021 cgctcgtcgg cgacggatcg tccccggggc actttgagag atacgtcttc gtggtgccga
     4081 agtcaacgcc aagaaccggc ttcgtgatca tgagcgccgc acctttctga cgagcccctt
     4141 cgccagcagt tcgccgttcc ggaagagcgg ctctcgctcc acgcgcaccg catcgccagg
     4201 ctccaccgcg ccgaaggatg tgtattttcc cgcggcctct tcattccatc ggaagagcac
     4261 ggccggattc tcatcggagt agcccaggtt gcgcacctcc gcggcaagct cggagacgcc
     4321 cgcggtcagg tcttcgcagc aggcgccttc aagggagagg attctcgacg cgaggagcgg
     4381 aatggcctcc tcttcgtcct tctgcagagg agcggacggc gacgccgcga gcagggcgac
     4441 gcgcgccgcc ccctcggtcc atgatgcaaa ggcacggcga acggactcct cgtgacgttt
     4501 ccggtcgaaa cgggtctcgc gcgctcccag gagcaagagc gttgcgagaa gcagcgggaa
     4561 aagcacgagc cccttgagcg acccgctctt ttcgcgcaga agcgaccacc ccttgaagaa
     4621 gatcgcgccg ccgctgagga agcggatgac ctctcttacg gtcgcgagcc ccagaatggc
     4681 ggccagcagc aggagcagct tccggctccg ggggagcagg agcgccgcaa gggttccgaa
     4741 gagcgcgccg agcggagccc cgatgaagac ccccgcggcc cggttgccaa ggagaatcca
     4801 ggtaagcgaa ggcaaaaacg ccataccggc gatcgttccg aaagcggatg ccgccgccgc
     4861 ccgaagtgga ggaatctcgg agagcaactc gggctccgga aggatgagcg cgggaaggcg
     4921 ctcttccatg cgcagcagaa gcagcgaacg ccgctccgga gggagcggtt cgtcggcgac
     4981 ggagtcaaga agcgcaggga gcagctcctc cgacgccgca gcctcggctc tcctgacggc
     5041 gctttcaagg gctctctgct ccggaacgcc gttcggcggc aagtacgcga agtagacgtt
     5101 gactcccttt tcgacggcgc gacgacggat ctcctcttct tcttttgttg taatcacagg
     5161 cgtttttctt ctcctgccca tcagaacgca ccctcttttt ctcttgattc caatagagta
     5221 gcgcctcgac cgtcgaagag caagcgtttt tcggccgggg gcacggaaat gatgtatcat
     5281 ggtatccgcg cctgtacgcg gcatccgcga aaggcgcgac agagaggagt gtactgagaa
     5341 tgagaacaga aacgcttcgg agaggaacga ggttcagcgt tatccttctc ttatgcgcgg
     5401 cggcgcttct ttcggccttc ggacaggcgg cctccgcgtc ggctttgacg gtgcgcgttc
     5461 tcgacgtcgg gcagggggat tcgattttcg tcgtttttcc ggagggcggc acgatgctcg
     5521 tcgacgccgg aaccgccgag gccggaccga gggtcgtcga gacgctccgt tcgctcgacg
     5581 ccgaaaagat cgacatcctt gtggcgacgc acccgcactc ggaccacatc ggcggtatgc
     5641 tcgccgttct ggacgccttc cctgtcggca aggtatggga cagcgggtat gtccacggga
     5701 cacgtacgca gcagcttttc ctacagacgg tgctcgaccg gaaaattcgt ttcgggcggc
     5761 cgaaggcggg cttttcggag gagtttcagg gcgtgctcat tgaagtgctg gcgcccgtga
     5821 agctgatcta tgggaccgac gccgacgtaa acaacaactc aatcgtcctc catctctcct
     5881 tcggagaggt ctccttcctg ctggccgccg acatggagac ggagcagcgg cgtacagtcc
     5941 gccgctttcc cgagacgacg gtacttaagg tcgcgcacca cggctcgcac aacggcacgg
     6001 acgcgcggtt tctcgcaatg ctccgcccga agacggccat catctcctgc ggcaagg
//
LOCUS       DAOTIL010000045         6043 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminobacteriaceae bacterium isolate AMR_MDS_3026
            NODE_15502_length_6043_cov_3.910655, whole genome shotgun sequence.
ACCESSION   DAOTIL010000045 DAOTIL010000000
VERSION     DAOTIL010000045.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868050
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminobacteriaceae bacterium (wastewater metagenome)
  ORGANISM  Aminobacteriaceae bacterium
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae.
REFERENCE   1  (bases 1 to 6043)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6043)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:19:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,224
            CDSs (total)                      :: 3,198
            Genes (coding)                    :: 3,184
            CDSs (with protein)               :: 3,184
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6043
                     /organism="Aminobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15502_length_6043_cov_3.910655"
                     /isolate="AMR_MDS_3026"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:3034609"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(12..605)
                     /locus_tag="P5201_01695"
     CDS             complement(12..605)
                     /locus_tag="P5201_01695"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRV97278.1"
                     /translation="MNTTDTAAVPSWRRSGSRRRIALLFLVLIPTITSSYTMARLLPE
                     RASLVLDALLVAVFGILFAWISVGFWAFMGGLFVLLTKRDRYRVSKSCDGRDLSIPDA
                     NARTAILIPIYNEEVNRVFAGVRTVYRSLKQTGALDRFDIFLLSDSTNPDVWVEEEEA
                     WHDFRREEEGTEQHVFYRKRDNNSKRKSGNVADFCRR"
     gene            complement(602..1009)
                     /locus_tag="P5201_01700"
     CDS             complement(602..1009)
                     /locus_tag="P5201_01700"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRV97279.1"
                     /translation="MTGLPKNIPADTHQERAAWRTSRDRVLLYLRGLDLPPFEDLDLA
                     AASLRACGAREESAAEGPGSPSPVEAMESLFNLLEEQRPEGLLGMRRIPSCPSLNRGV
                     MVAEPMDRVPWKTAFVRWMRRWRRELFAREGAR"
     gene            complement(1227..3329)
                     /gene="mdoH"
                     /locus_tag="P5201_01705"
     CDS             complement(1227..3329)
                     /gene="mdoH"
                     /locus_tag="P5201_01705"
                     /EC_number="2.4.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013049195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucans biosynthesis glucosyltransferase MdoH"
                     /protein_id="HRV97280.1"
                     /translation="MTAFEIDWSRSAGRRRLLLIFLVLAPTFIACGTMASILPSRGGS
                     PLEIVLLAVFAVLFAWISVGFWTALMGFFLLLQRRNRYSVSNVCDGCGFSIENPQART
                     AVLIPIYNEDVKRVMAGLKTVYDSLAATGSLDRFDFFVLSDTTDPDVWVQEEEGWHRF
                     CTEQDAFGRVFYRKRRSNAKRKSGNVADFCRRWGKNYRYMIVFDADSIMAGRTMVRMV
                     QMMEARPDIGILQTPPAGVNRETLLARAQQFANRVYGPMFAAGLHFWQLGDAQYWGHN
                     AIIRVEPFIRHCELPRLPGKGPLGGDILSHDFVESALMRRAGYGVWLAYDLDGSYEET
                     PPTLLDELKRDRRWCQGNLQHLRLIFSRGFFPAHRALFLNGILSYGSALLWLAFLLIS
                     SAQAVAEVYLEPVYFLDTKTLFPQWPVWRPHWALALLGSTAVLLFLPKFFSYALALLR
                     DPAARLYGGRLRLACGILLEVALSTLLAPVRMLHHSVFILSTILGKNVGWPTQSRDDR
                     GTPWADAVDVHWWGTILGILWGALVYLTNPSFFWWISPIILSLVLSIPLSVLTSRASL
                     GKAFLRAGFFTTPEETEPPVELRELAKTLQEEAPYSPLPLSPRQGFLRAVTDPCVHAL
                     HTSLLLRYKKGAPAREEARQALVEKALRSGPDSLSSKEKMTLLNDPFHLAELHRRVWE
                     LEDDKQAARWGLSQPSVD"
     gene            complement(3326..3682)
                     /locus_tag="P5201_01710"
     CDS             complement(3326..3682)
                     /locus_tag="P5201_01710"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRV97281.1"
                     /translation="MTSIRDTDRWRISRDRVLLYVRALNLPPYKGLDLAVESLKRTGP
                     SSAAESMESLRALLKEHGLDRGVLDSEGKHLASVPPLNRSVMVPEELDRVPWRTSFVQ
                     FLRRWKNDLFEKGRAR"
     gene            complement(3666..5336)
                     /locus_tag="P5201_01715"
     CDS             complement(3666..5336)
                     /locus_tag="P5201_01715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005660018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030246 - carbohydrate binding [Evidence
                     IEA];
                     GO_process: GO:0009250 - glucan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucan biosynthesis protein G"
                     /protein_id="HRV97282.1"
                     /translation="MYHKMFHRSAYHRAVAVLMAAVSLFLHAPAHAEGAVSSPGEIPP
                     PKVIPAEAFSFDNVVKKAEELAARPFENPDGAVPDFLLNISYDQWRKIRFRPNMSLWR
                     NEKLPFEAQFFHPGLFYNRLVGINIVHDGGALRLPFSTNFFEYGDNAFASKTGSVPLD
                     FTGFRLHFPLNRDDYKDEVAVFLGASYFRAVARGTQYGLSARGLAVDTAMQSGEEFPY
                     FREFWIEKPAQGAEQIAVCALLDSPSLAGAYRFILTPGEETVMDVECMLFLRRDVQKI
                     GIAPLTSMYLYGEPENGRDGDFRPEVHDSDGLLYHSEEGEWTWRPLANPKRLAVLSAP
                     LQNPRGFGLMQRDWLFDHYQDLEARYEKRPSLWIEPLSEWGEGNVELVEIPTEQEIHD
                     NIVAYWVPAKTPETAEGRPKDETPEYPRTMTFAYRMRWLQPHRPVHELGRVMATRIAA
                     GNSPEMMKFVLDFEGDELSELPAGAGLTSIVTVGDGARLIEKQLQKNEATGGWRLVFQ
                     VAPEAESGLKTLFPSPAGRPRIRLSALLKRGENIPEPLTETWTYDLHP"
     gene            complement(5440..>6043)
                     /locus_tag="P5201_01720"
     CDS             complement(5440..>6043)
                     /locus_tag="P5201_01720"
                     /inference="COORDINATES: protein motif:HMM:NF012772.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HRV97283.1"
                     /translation="EDIGEINSLGRFVLERSCLQAKEWRGEGRDIVMCVNVSAKQLAS
                     ERFVHSVLGALERTGTPPHALELEITESAFMSNPEYATRVMEELKERGLRFTLDDFGT
                     GYSSLSYIRSLPLSGIKIDRSFTADLDNPKTRAIVSTIMHMAGELNLELTVEGIETTA
                     ELNTIRSLAPRGGDILLQGYLFSRPIPPDTFSSLFDRDFM"
BASE COUNT         1141 a         1833 c         1975 g         1094 t
ORIGIN      
        1 tagttcttcc cctagcggcg gcagaagtcc gctacgttgc cgctcttccg cttgctgttg
       61 ttgtcgcgct tccggtagaa gacgtgttgc tccgtcccct cctcttcgcg gcggaagtcg
      121 tgccacgcct cctcctcttc cacccagacg tcggggttgg tgctgtcgct gaggaggaag
      181 atgtcgaagc ggtcgagggc gcctgtctgt ttcagcgagc ggtagacggt gcgcaccccc
      241 gcgaagacgc ggttcacctc ttcgttgtag atggggatga ggatcgcggt tctcgcgttc
      301 gcgtcgggaa tggagaggtc gcgtccgtcg cagctcttcg agacgcgata acggtcgcgc
      361 ttcgtcagga gaacgaagag ccctcccatg aaagcccaga aacctaccga aatccacgcg
      421 aagaggatgc cgaacacggc tacgaggagc gcgtcgagta cgaggctcgc gcgttcgggg
      481 agcaggcggg ccattgtgta gctcgacgtg attgtgggaa tgaggacgag gaagagcagc
      541 gcgatgcgcc gtctgcttcc ggaacgtctc caggagggga ctgcggcggt gtcggttgtg
      601 ttcatctcgc gccctccctg gcgaagagtt cacggcgcca gcgccgcatc cagcggacga
      661 acgccgtctt ccacgggacg cggtccatcg gttcggcgac catcacgccc ctgttcagag
      721 aagggcacga ggggattcgc cgcatgccga gcagcccttc cggacgctgc tcttcaagca
      781 ggttgaagag cgactccatc gcctcgacgg gggacggtga gcccgggcct tccgcagcgg
      841 attcctcgcg cgctccgcaa gcgcggaggc tagcggcggc gaggtcgagg tcttcaaaag
      901 gcggcaggtc cagtccgcgc agatagagca gcacgcggtc gcgcgaagtc cgccacgcag
      961 cgcgctcctg atgagtatcc gccggaatgt tctttgggag ccccgtcatc gtgcttcctc
     1021 ctccgcaaag ccttctgctg aagaaagccc cgcagccgcg ggaaagggaa gagaacgcgg
     1081 ctgtgttgtg gcggcgtgtc cggtgcagtg cataaaatcc tctccttgat cgtgtgggtg
     1141 tttttattga gtatacttcc ttttggttga agagagaagg taaaaaacag tgagaggagc
     1201 aaattccgct cctctcacgt gcgtgttcag tcgacggacg gctgcgagag cccccaccgg
     1261 gcggcctgtt tgtcgtcctc cagctcccag acacgcctgt gaagctcggc gaggtgaaag
     1321 ggatcgttca ggagcgtcat tttctccttt gaggaaagag agtcgggacc ggaacgaagc
     1381 gctttctcca cgagggcctg ccgcgcctcc tcccgcgcgg gcgctccctt tttgtagcgc
     1441 agcagcagcg acgtatggag cgcgtggacg cacgggtcgg tcacggcgcg gaggaatccc
     1501 tgccgtggtg acagaggaag aggtgagtac ggcgcttcct cttgaagggt tttcgccagc
     1561 tcgcgcagct ccaccggcgg ttccgtctcc tccggagttg tgaagaaccc cgcccgcagg
     1621 aacgccttcc ccaggctcgc cctgctcgtg aggacggaga gcgggatgga gagtacgagc
     1681 gagaggatga tgggcgatat ccaccagaag aaggaggggt tcgtcaggta gacgagcgcg
     1741 ccccagagga tgccgagtat cgtcccccac cagtgaacgt cgacggcgtc cgcccatggg
     1801 gttccccggt cgtcgcggga ctgggtcggc caaccgacgt ttttgcccag aatggtcgag
     1861 aggatgaaga cgctgtgatg cagcatccgc acgggggcca gcagcgtgga gagcgccacc
     1921 tccagcagaa tgccgcaggc gagccggagc ctccccccgt aaagacgcgc cgccggatcg
     1981 cgcagaagcg ccagcgcata gctgaagaac ttggggagga agagcagcac cgcggtcgag
     2041 ccgaggagcg cgagcgccca gtgaggcctc cagaccggcc actgcgggaa gagcgtcttt
     2101 gtgtcgagga agtagacagg ctccaagtag acctctgcca cggcctgcgc tgagctgatg
     2161 agcaggaagg ccagccagag cagcgccgaa ccgtacgaga ggatgccgtt caggaagagc
     2221 gcgcggtgcg ccggaaaaaa accccgcgaa aagatcaacc gtaggtgctg caagttgccc
     2281 tggcaccagc gccggtcgcg cttcagctcg tcgagcaggg tcggcggcgt ctcttcgtag
     2341 ctgccgtcga ggtcgtacgc cagccagacg ccgtacccgg cgcggcgcat gagggccgac
     2401 tccacgaagt cgtggctcag aatgtcgccg ccgagcggcc cctttccggg gaggcgcggc
     2461 agctcgcagt gcctgatgaa cggttctacc ctgatgatcg cgttatggcc ccagtactgc
     2521 gcgtcgccga gctgccagaa gtgcagtccc gcggcgaaca tcggcccgta gacgcggttg
     2581 gcgaactgct gcgcccgcgc gagcagcgtc tcgcggttca ctcctgcggg cggcgtctgc
     2641 agaatgccga tatcgggccg cgcctccatc atctgtacca tacggaccat cgtccgtccc
     2701 gccataatgc tgtcggcgtc gaagacgatc atatagcggt agttcttccc ccagcgcctg
     2761 cagaagtcgg ccacgtttcc gctcttccgc ttcgcgttgc tccggcgctt ccggtagaag
     2821 acccgcccga aggcgtcttg ctccgtgcaa aaacgatgcc agccctcttc ctcctgtacc
     2881 cagacgtccg gatcggtagt gtcgctcagg acgaagaagt cgaaccggtc gagcgacccc
     2941 gtcgcggcaa gcgaatcgta gacggtcttc aggccggcca tgaccctttt tacgtcctcg
     3001 ttgtagatcg gtatcaggac ggccgtccgg gcctgcgggt tctctatcga aaagccgcac
     3061 ccatcgcaga cgttggagac cgagtagcgg ttgcgccgct gcagaaggag gaagaagccc
     3121 atcagcgcag tccagaagcc aaccgagatc catgcgaaga gcaccgcgaa gacggcgagc
     3181 agcacgatct ccagagggct tccaccgcgc gaaggcagga tggacgccat cgtcccgcac
     3241 gcaataaagg tcggcgcaag gacgaggaag atcagcagca gcctcctccg gcccgcgctg
     3301 cggctccagt cgatttcgaa cgcggtcatc gggcgcgccc cttttcaaaa aggtcgttct
     3361 tccagcggcg gaggaattgg acgaacgacg tcctccacgg aacgcggtca agctcctcgg
     3421 gaaccatcac gctccggttg agcggcggga cggaggcgag atgcttccct tcggagtcaa
     3481 gcacgccccg gtcgagcccg tgctctttca aaagcgcgcg gagcgactcc atcgactccg
     3541 ccgcggagga cgggccggtg cgtttcaggc tttcgacggc gaggtcgagc cccttgtagg
     3601 gcggcaggtt gagcgcgcgc acgtagagca gcaccctgtc gcgggagatg cgccagcggt
     3661 cggtatcacg gatggaggtc ataggtccag gtctccgtca gcggttccgg gatattctcc
     3721 ccccgcttca gcagcgcgga gaggcggatg cgggggcggc cggcgggcga aggaaagagc
     3781 gtcttgagac cgctctcggc ttcgggggca acctggaaga ccagccgcca ccctccggta
     3841 gcctcgttct tctgaagctg tttctcgata aggcgcgcgc cgtccccgac cgtcacgatg
     3901 cttgtcaggc ccgctcccgc cgggagttcc gaaagctcgt ccccttcgaa gtcgaggacg
     3961 aacttcatca tctccgggga gttccccgcc gctatgcgcg tcgccatgac ccggccgagt
     4021 tcgtggaccg ggcggtgcgg ctgaagccag cgcatcctgt aggcgaatgt catcgtccgg
     4081 ggatactccg gcgtttcgtc ctttggccgg ccctcggcgg tttcgggcgt cttcgcaggg
     4141 acccagtagg cgacgatatt atcgtggatc tcctgttcgg tcgggatctc cacaagttcc
     4201 acattcccct cgccccactc gctgagcggc tctatccaga gcgacgggcg tttctcatac
     4261 cgcgcctcaa ggtcctggta gtggtcgaag agccagtccc gctgcatcag accgaaaccg
     4321 cgcgggtttt gaagcggcgc ggagagcacg gcgaggcgct tcggattcgc cagcggccgc
     4381 cacgtccact ccccctcttc gctgtggtac agcagtccgt ccgaatcgtg cacctcggga
     4441 cggaagtcgc cgtcgcggcc gttctcaggt tcaccgtaga ggtacatgct cgtgagcggc
     4501 gcaataccga tcttctggac gtctcttcgc agaaagagca tgcactccac gtccatgacg
     4561 gtctcctcgc cgggagtcag aataaaacgg taggccccgg caagactcgg actgtcgaga
     4621 agcgcgcaca cggcgatctg ttcggcgccc tgcgcaggtt tttctatcca gaactcccgg
     4681 aagtacggaa attcctcgcc ggactgcatt gcggtgtcga cggcgagtcc gcgcgcggag
     4741 agaccgtact gcgtgccgcg cgccaccgct ctgaagtagc tcgcgccgag gaacacggcg
     4801 acctcgtctt tataatcgtc cctgtttaag gggaagtgca gccggaatcc ggtgaagtcc
     4861 agcggaaccg accccgtttt cgacgcgaag gcgttgtctc cgtactcgaa gaagttcgtg
     4921 gagaatggaa gtcgcagcgc ccctccgtca tggactatgt tgattccgac gagcctgttg
     4981 tagaagaggc cggggtggaa gaactgcgcc tcgaagggca gcttctcgtt ccgccacagg
     5041 gacatgttcg ggcggaagcg gatctttcgc cactggtcgt agctgatgtt gagcaaaaag
     5101 tcgggaaccg ccccgtcggg attttcaaag ggacgggcgg cgagttcttc ggccttcttc
     5161 accacgttgt caaaagaaaa ggcttcggcc gggatgactt tcggcggggg aatctcgccg
     5221 ggggacgaaa cagccccctc ggcgtgcgcg ggagcgtgga ggaaaagaga caccgccgcc
     5281 atgagaacgg cgacggcacg atggtaggcg cttctgtgaa acattttgtg atacaagaca
     5341 caagaccctc cttggccgga gtatccggac cggttgtgaa agcagataca tcatagtcat
     5401 ttttggatgg agcgcaaggg gggtgaaaag agagactttt tacatgaagt ccctgtcgaa
     5461 gagtgatgaa aaagtatccg gcggaatcgg acgagagaag agatacccct ggagcagaat
     5521 atctcccccg cgcggggcca gcgaacggat ggtattgagt tcggctgtcg tttcgattcc
     5581 ttcgacggtg agctccaggt tgagttcgcc cgccatgtgc attatcgtcg aaacgatggc
     5641 gcgcgtcttt ggattgtcga ggtcggcggt gaaggagcgg tcgatcttga tgcccgagag
     5701 cgggaggctg cggatatagg agagcgagga gtacccggtg ccgaagtcgt cgagggtgaa
     5761 gcgcagcccg cgctccttga gctcctccat tacacgcgtc gcgtactcgg ggttcgacat
     5821 gaaggcggat tcggtgattt ccagttccag cgcatgcggg ggcgtcccgg tgcgttcaag
     5881 cgcgcccagg acggagtgga caaatcgctc cgaggcaagc tgttttgccg agacgttcac
     5941 gcacatgacg atatccctcc cctcgccgcg ccactccttc gcctggaggc aggagcgttc
     6001 aaggacaaat cttccgagcg aattgatctc gccgatgtcc tcc
//
LOCUS       DAOTIL010000046         5957 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminobacteriaceae bacterium isolate AMR_MDS_3026
            NODE_15833_length_5957_cov_3.019485, whole genome shotgun sequence.
ACCESSION   DAOTIL010000046 DAOTIL010000000
VERSION     DAOTIL010000046.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868050
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminobacteriaceae bacterium (wastewater metagenome)
  ORGANISM  Aminobacteriaceae bacterium
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae.
REFERENCE   1  (bases 1 to 5957)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5957)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:19:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,224
            CDSs (total)                      :: 3,198
            Genes (coding)                    :: 3,184
            CDSs (with protein)               :: 3,184
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5957
                     /organism="Aminobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15833_length_5957_cov_3.019485"
                     /isolate="AMR_MDS_3026"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:3034609"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1174)
                     /locus_tag="P5201_01725"
     CDS             complement(<1..1174)
                     /locus_tag="P5201_01725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009201516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HRV97284.1"
                     /translation="MDPSKLTESILESRTHPVRTGALPDQDPAAESGEVSSLRSGGTA
                     DLDRKSSGKLRDSYIGRIPEEERAISVKFAWHELWSKVIVYLSREELMQLGEALVMAG
                     AAHKEQRRSTGDPYIVHTINVASILADMQLDNVTLMAALLHDVLEDTDITMEKLTGAF
                     GAPVAMLVDGVTKLGKLPFKSFEDYQAENLRKMFVVMAKDIRVVLIKLADRLHNMRTL
                     GALRKDKQQRIARETLEIYAPLAHRLGIYQVKRGLEDLAFKYADPEMYYEIRRRVRKK
                     LPERETVVKKALEVLTARLEEEGIRCKVKGRAKHFYSIYEKMNRKKLPVEQLYDLLAL
                     RVMVDDIAACYTVLGIVHTIWKPIPGQFDDYIANPKSNMYQSLHTTVVGPTGEPLEV"
     gene            complement(1234..2952)
                     /locus_tag="P5201_01730"
     CDS             complement(1234..2952)
                     /locus_tag="P5201_01730"
                     /inference="COORDINATES: protein motif:HMM:NF013528.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DHH family phosphoesterase"
                     /protein_id="HRV97285.1"
                     /translation="MTALCRSASLRLFQPDSSTVELADRIGCSLLTAAVLESRNLAAA
                     DERAPSLPDASGVESARESLFLDAKIPFEDLMEELSLGENAAEAADRWRSLKTLGRVL
                     VYGDYDADGVSSAALALELCRGKAERARFFIPHRHEQGYGLHESVLDQLLDIGWDTLV
                     VVDCGTKDGEILEKVARSGVNVFVFDHHLPEEGAPLHSAVVNPHTCAPRSAPGENEAG
                     RALCATGVLWAWAWKHSLLPRKRLAAMTDLVALATIADCMPLTPLNRALVRQGMDILS
                     TTPRKGLARLFSRLGIARPGSALTEETLSMKVIPCINAAGRMDFADTAVNVLLGSPNV
                     DESVEMLISLNRKRQSLSLKISKEAELVLSGGVNVILGESWPVGVLSGVASRICGATG
                     APVALAAPVNGGIRGTLRVPEGADAVGVLSALSSKLEAWGGHKYAAGFSVSRENWDFV
                     RRDLQQALSSMKVEETSVTAIAMAPEEISIESWKEACTLGPFGNGNPPPMFFLARKGR
                     ERMLPLGKDGKHIQIDAGGIRLLAFHGENEREALAGADGWVYRPRIDCWRGEERLQYV
                     VDYAVV"
     gene            complement(3283..4779)
                     /locus_tag="P5201_01735"
     CDS             complement(3283..4779)
                     /locus_tag="P5201_01735"
                     /EC_number="2.4.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014162541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003825 - alpha,alpha-trehalose-phosphate
                     synthase (UDP-forming) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trehalose-6-phosphate synthase"
                     /protein_id="HRV97286.1"
                     /translation="MKKKERLLVVSNRLPVALSTEDGQLTGRPASGGLVNALSPVLKN
                     KGGFWIGWAGTHENVSLSTLREILGPVSETSGYRLLPVMLTKEDVDSFYSGFSNSVLW
                     PLFHDLQSRCDFNPSYWDGYLAANRKFAKVLMKHTEEHDFIWVNDYHLIPLGKLLREK
                     KPSLKASFFLHIPFPSVDIFMKMPQRKEILSQLVQYAFVGFQTMRDRRNFLDCLKVFF
                     PEMQVSGRGNVVRVRIGESNFLVGALPISIDYRSFASLASSKEVISRSKDIQREMFAQ
                     KYILGVDRLDYSKGIPERLRGFQRCLERYPELREKLSLMQVVVPSRESVGEYLDLKQE
                     IELLISRINGEYSTMRWVPVHWRYGSVPRSELAALYRGAEMALVTSLKDGMNLVCKEY
                     CACKGDGSGVLVLSEFAGAAIQLSKGALLVNPYDVDAVAAAIAEGFAMDAHEKKRRMR
                     LLRKSIARHDVFRWVDDFLRASVGKKLAEFPEEEIPPLWPGLESDGEK"
     gene            complement(4882..>5957)
                     /locus_tag="P5201_01740"
     CDS             complement(4882..>5957)
                     /locus_tag="P5201_01740"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012869050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HRV97287.1"
                     /translation="KELGLDVSWEVIEGNPDFFACTKTLHNSLQGMPGGLPEQQIEAY
                     VETNRTNAEKLRGVVEDADYVFIHDPQPAPLLGYFPKRKGKWIWRCHIDVSSPNRSVW
                     RFVKKHVEGYDASIFSLPDFAQTLPHPQFLITPSIDPLSEKNIALPKSEVAKTYEELG
                     IPMDKPVILQVSRFDRFKDPLGVIESFRIAREHVDIRLVLAGGGATDDPESGEVLARV
                     QEAAAGDPDIHVLLLPADAHRTINALQSGADVVMQKSTKEGFGLTVTEGLWKKKPVIG
                     GDVGGIRLQIINRFNGFRVRSPEGAAIRIRYLLTHKWKRERMGVNARQYVLENFLITG
                     QLRRYLSLLVSLNSPDGEWERWI"
BASE COUNT         1183 a         1875 c         1663 g         1236 t
ORIGIN      
        1 gcacttcgag aggttccccg gtcggtccga ccaccgtagt atgaagcgac tggtacatgt
       61 tcgacttcgg attggctatg tagtcgtcga actgccccgg aatcggcttc catatcgtgt
      121 ggacgatgcc gagcaccgta tagcatgcgg cgatatcgtc caccatcacc cggagcgcaa
      181 gcaggtcgta gagctgctcc acggggagct tcttcctgtt catcttttcg tagatactgt
      241 aaaagtgctt cgcgcgcccc tttaccttgc agcggatccc ttcctcttca agccgggcgg
      301 tcagcacttc gagcgccttc ttgaccacgg tctcccgttc ggggagtttt ttccggacgc
      361 ggcgtctgat ttcgtagtac atctcgggat cggcgtactt gaatgcgaga tcctcaagtc
      421 cgcgtttcac ctgataaata ccgaggcggt gcgcaagcgg cgcatagatt tcgagcgtct
      481 cgcgcgcgat gcgctgctgc ttgtccttcc tgagggctcc cagagtgcgc atgttgtgga
      541 gacgatccgc gagcttgatg agcaccacgc ggatatcctt ggccatcacg acaaacatct
      601 tccgcaggtt ttccgcctgg taatcctcga acgacttgaa ggggagctta ccgagctttg
      661 tcacgccgtc gaccagcatc gccacaggag cgccgaaagc gccggtcagt ttctccatgg
      721 tgatgtcggt atcctccaga acgtcgtgaa gcagggcggc catcagcgtg acgttgtcga
      781 gctgcatatc cgcaagaatg gaagcgacgt tgatcgtgtg gacaatgtac ggatcgccgg
      841 tcgagcggcg ctgctctttg tgggcggcgc ccgccattac gagcgcctcg ccgagctgca
      901 tcagctcttc cctggagaga tagacgatca ccttcgacca gagctcgtgc catgcgaact
      961 tgaccgatat tgccctctct tcctccggaa tacggccgat atagctgtcg cggagctttc
     1021 ccgagctctt cctgtcaaga tccgccgttc ccccggagcg caacgaagaa acctcgccgc
     1081 tctccgctgc ggggtcctgg tccggcaacg ctcctgtacg aacgggatgc gtccggcttt
     1141 ccagaatact ctcggtcagt ttactggggt ccatttttcc ctccgctcac atctggaacc
     1201 gatcgcttcc aaagaactcc ggggtgcgcc ttgctaaaca acggcgtagt cgacaacgta
     1261 ctgcagacgc tcttcgcctc gccagcagtc gatccggggg cggtacaccc atccgtccgc
     1321 tcctgcgagc gcctcccgct cgttctcccc gtgaaaagcg agaaggcgaa tcccgcccgc
     1381 gtcaatctgg atatgtttgc cgtccttgcc gagcgggagc attcgctccc ttcccttgcg
     1441 ggcgagaaaa aacattggag gcgggttgcc gttgccgaat ggaccgagcg tgcacgcttc
     1501 cttccaggac tctatggata tctcctccgg cgccatcgca atcgccgtca cagacgtctc
     1561 ctcgaccttc atcgaggaaa gcgcctgctg gagatcgcgc cggacaaaat cccagttctc
     1621 cctggagacg gaaaaaccgg cggcgtactt gtgccctccc cacgcctcca attttgaaga
     1681 gagggcggag agcacgccga cggcatcggc gccttcagga acgcggagcg ttcctcgtat
     1741 gccgccgttc acaggggccg caagcgccac cggagctccc gtggcgccgc aaatcctgct
     1801 ggcgacgccg ctgagcaccc cgaccggcca ggactccccg agtatgacgt tcacgcctcc
     1861 ggagagcacc agctccgcct ctttcgatat cttcagcgaa agcgactggc gcttccgatt
     1921 cagcgaaatc agcatctcca cggattcatc cacgttcgga gaaccgagga gcacgttcac
     1981 cgcagtatcg gcgaaatcca tcctcccggc ggcgttgatg cagggaatca ccttcatcga
     2041 gagggtctcc tccgtcagcg cgcttccggg gcgggcgatc ccgagtctcg aaaaaagccg
     2101 tgcgagcccc ttgcgggggg ttgtcgagag aatatccata ccctgccgga caagcgccct
     2161 gttgagcggc gtcaacggca tgcagtccgc tatcgtggcg agcgcgacga gatccgtcat
     2221 cgccgcgaga cgttttcggg gaaggagcga gtgcttccat gcccatgccc agagcactcc
     2281 ggtagcgcac agcgctctgc cggcctcgtt ctcccccggc gccgaccggg gcgcgcacgt
     2341 atgcggattc accaccgcgc tgtgaagcgg cgccccctct tcgggaaggt ggtggtcgaa
     2401 gacgaagaca ttgacgcctg aacgcgcgac cttctccagg atctccccgt ccttcgtgcc
     2461 gcagtccacc acgacgagcg tatcccagcc gatatcgagg agctgatcaa gcaccgattc
     2521 gtgaagtccg tacccctgct catggcgatg aggaataaaa aaccgggcgc gctcggcctt
     2581 cccacggcaa agctccaggg caagcgcagc cgacgacacc ccgtccgcgt cgtaatcgcc
     2641 gtacacaagc accctgccga gcgttttgag gctccgccag cgatctgccg cttcggcggc
     2701 gttctcccca agagacagct cctccatgag gtcttcgaac ggtatctttg catcgagaaa
     2761 cagactctca cgggcactct ccactccgga agcgtccgga agagatgggg cccgctcatc
     2821 ggcagcggcc aggttgcggc tctcaaggac agccgctgta agcaacgaac agccgattct
     2881 gtccgcaagc tccacggtcg aggagtcggg ctgaaagaga cgaagcgaag cggaacgaca
     2941 gagcgccgtc acaggactcc gttccctttc cgttccgcac tgtttttttg gacgcaccgt
     3001 acgccgttcc gggcccggat gtcattcgca catctttctg ctgtctgaaa atacaccata
     3061 aatacatcct catttcacca tatatcgtaa cggaagacgt gcatcactcc atcttccggc
     3121 cggccgccgg tttttccgtg cgtgtattcc gggcagagct ctcagcggcg ctccgcagaa
     3181 aactgcggag cctgctcacg atgaaccgtg cagctccgct tcatttctat ccttttgttt
     3241 attgtagtcg gtgaggatgg agaagtaaac tcccgaaaga atctacttct caccatcgga
     3301 ctcgagcccg ggccagagcg gaggtatctc ctcctccggg aactcggcaa gcttttttcc
     3361 cacggaagcg cgcagaaaat cgtccaccca gcgaaagacg tcgtgccgtg caatcgattt
     3421 ccggagaagg cgcatccgcc gcttcttttc atgtgcatcc atcgcaaatc cttcggcgat
     3481 cgccgccgcg acggcgtcga catcatacgg attcacaagc agggccccct tggaaagctg
     3541 gattgccgcg ccggcgaact cgctcagtac gaggacgccg cttccgtctc ccttgcaggc
     3601 gcaatactcc ttgcacacga gattcatccc gtctttcaga gaggttacaa gggccatctc
     3661 cgcgccgcga tagagcgcgg caagttccga acgcggaacg gagccgtagc gccaatgcac
     3721 cggaacccac cgcatcgttg aatactctcc gttgatcctg ctgatgagca gctcgatctc
     3781 ctgcttcagg tcaagatact cacccactga ctccctgctc gggacgacga cctgcatcag
     3841 gctgagtttt tcacgaagct cgggataacg ctccagacag cgctgaaaac cgcgcagccg
     3901 ttcgggaatt cccttcgagt agtcaagacg gtcgacgcca agaatgtatt tttgggcgaa
     3961 catctcccgc tggatgtcct tcgagcggga gatcacctct ttcgaggagg caagggaggc
     4021 gaacgaccgg taatcgatgc tgatcggcag ggcgcctacc aggaaattgg attctccgat
     4081 gcggacgcgc accacgtttc cccttccgga aacctgcatc tccgggaaga agaccttcag
     4141 gcagtcaagg aagttcctcc tgtcccgcat cgtctggaat ccgacaaaag catactgaac
     4201 cagctgcgat agaatctctt ttctctgcgg catcttcatg aagatgtcca cgctggggaa
     4261 cgggatgtgt aaaaagaatg aagccttgag cgagggtttc ttctcgcgca gcagctttcc
     4321 gagcgggatc aggtggtagt cgttgaccca gatgaaatca tgttcctcgg tatgtttcat
     4381 cagcaccttc gcgaattttc ggttcgctgc gaggtatccg tcccagtagg agggattgaa
     4441 gtcgcacctg ctctgaaggt cgtgaaagag cggccagagc acggaattcg agaatccgga
     4501 atagaaggaa tccacgtcct ccttggtcag catcaccgga agaaggcgat aaccgcttgt
     4561 ctcggaaacg gggccgagaa tttcgcggag cgttgaaaga gaaacattct cgtgcgttcc
     4621 tgcccacccg atccaaaacc cccccttgtt cttcagcaca gggctcagcg cgttgacgag
     4681 tccgcccgaa gccggacgcc cggtcagttg tccgtcctcc gtcgagagag cgacgggaag
     4741 gcggtttgaa accaccagca gacgttcttt ctttttcatc gttttatttc ccttcttccc
     4801 tttgtatctt catgagcgcg tccctccaga gcgctacgtg aagcgcacgc cgccgtcctt
     4861 tcgttcacac gtccctgccg gtcagatcca tcgctcccac tcgccatccg gcgaattcag
     4921 ggagacgagg agcgacaggt agcgccgcaa ctgcccggta atgaggaagt tctcgaggac
     4981 gtactgccgt gcgttcactc ccatgcgctc ccgcttccac ttgtgtgtga gaagataccg
     5041 gatacgaatg gccgcgcctt cgggggagcg cacgcggaac ccgttgaaac ggttgatgat
     5101 ctgcaggcgt attccgccga cgtcccctcc gataaccggc tttttcttcc acaagccttc
     5161 ggttactgta aggccgaagc cctccttggt cgatttctgc atcactacgt cggcgccgga
     5221 ctgcagggcg ttgatcgtcc tgtgcgcatc ggctggaaga agcaagacgt gaatgtccgg
     5281 gtcgcccgcc gccgcctcct gaaccctggc gagcacctcg cccgattcgg ggtcgtcggt
     5341 cgccccgccc ccggcgagca caaggcgaat atccacgtgc tcgcgggcaa ttctgaagct
     5401 ttcaataacc ccgagggggt ccttgaagcg gtcgaaccgc gagacctgaa ggatgaccgg
     5461 tttatccata gggataccga gttcctcgta ggtcttcgca acctcgcttt tcggcagcgc
     5521 gatattcttt tcgctcaggg ggtcgatgct cggcgtgatc agaaactggg ggtgcgggag
     5581 cgtctgggcg aaatcgggaa gcgagaagat cgacgcgtcg tacccctcca cgtgcttctt
     5641 gacaaaccgc cagaccgagc ggttcggcga gctgacgtcg atatggcagc gccaaatcca
     5701 ctttcccttt ctcttcggaa aatacccgag cagcggcgcg ggctgcggat cgtggataaa
     5761 gacataatct gcatcctcaa ccacgccccg cagcttctcc gcgtttgtac ggtttgtctc
     5821 gacgtacgct tcgatctgct gttccggcag tccccccggc atcccctgca aagagttatg
     5881 aagggttttt gtacaggcga aaaagtcggg attcccttcg atgacctccc aggagacgtc
     5941 gagtccgagc tccttcg
//
LOCUS       DAOTIL010000047         5947 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminobacteriaceae bacterium isolate AMR_MDS_3026
            NODE_15880_length_5947_cov_2.970468, whole genome shotgun sequence.
ACCESSION   DAOTIL010000047 DAOTIL010000000
VERSION     DAOTIL010000047.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868050
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminobacteriaceae bacterium (wastewater metagenome)
  ORGANISM  Aminobacteriaceae bacterium
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae.
REFERENCE   1  (bases 1 to 5947)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5947)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:19:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,224
            CDSs (total)                      :: 3,198
            Genes (coding)                    :: 3,184
            CDSs (with protein)               :: 3,184
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5947
                     /organism="Aminobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15880_length_5947_cov_2.970468"
                     /isolate="AMR_MDS_3026"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:3034609"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            131..1567
                     /locus_tag="P5201_01745"
     CDS             131..1567
                     /locus_tag="P5201_01745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014163720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-linked oxidase C-terminal domain-containing
                     protein"
                     /protein_id="HRV97288.1"
                     /translation="MSVQAYSPVTEQTVEELQKLIGASNVSADLEKRIAYSYDEVPRF
                     YWDKEYIAEVVVFPETTEDVSAVMRYANDRRIPVTPRGAGTGLSGGAVPFSGGIVLSF
                     EKMRAIIELDKKNLTITVEPGVVTAEITKAAEREGLLYAGDPCSGDASFIGGNVAENA
                     GGNKVVKYGTTGNSVLALEAVLPDGTATWFGGKRRKDATGYDFAHLIVGSEGTLAVVT
                     KIILKLLPLPGKVLDLLVPFPDVASAIDFAPRVMSEGKVIPSSMEFMDRRSIKLAERF
                     LNTGLPFDDAGAHLIIQLEGNDPEVLADDMERIGDLCFAHGALEVFVADNRTTRDKLW
                     KARKCLAEAIMAFYPKYSLEDLVVPTSEIPKLMAETTRLCTKYGLEEANFGHVGDGNM
                     HVNILFPEERPDWHSIVDELLDELYDFTASIGGTLSGEHGIGLKRQKYMCRVMDASQM
                     ELIHRVKLAFDPNNILNPGKMISCPDRE"
     gene            complement(1619..>2595)
                     /locus_tag="P5201_01750"
     CDS             complement(1619..>2595)
                     /locus_tag="P5201_01750"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: protein motif:HMM:NF012403.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HRV97289.1"
                     /translation="KRSAAAMRLKGKFRIITTGTPIENHLGELWNLFRFLNPGLLGSL
                     DSFNRRFATPIVKYDDKGARARLKKILRPFLLRRTKDQVLDELPPKTEITLRVDMNPE
                     ERSLYEALRRNALERIEKGDGEDQRFVILAELMKLRRACCNGALVLPDREFPSAKLEA
                     FGEILEDLRANGHRCLVFSQFVDHLAILRASLNEAGVSYSYLDGSTPPEERKRQVEAF
                     QAGEKECFLISLRAGGTGLNLTAADYVVHMDPWWNPAVEDQASDRAHRIGQERPVTVY
                     RIVAKDTIEEKIVDLHTTKRDLAESLLEGTERVTSFSLNEMAALIRQE"
     assembly_gap    2596..2605
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<2606..5068)
                     /locus_tag="P5201_01755"
     CDS             complement(<2606..5068)
                     /locus_tag="P5201_01755"
                     /inference="COORDINATES: protein motif:HMM:NF012403.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0140658 - ATP-dependent chromatin
                     remodeler activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SNF2-related protein"
                     /protein_id="HRV97290.1"
                     /translation="MIPDILKKTKGGASGSSLPNDELLVHQAESLILSGDLAAATEKI
                     GGAVSGESYAVRALLALLDDRRKDALALFEEGLKRLRKERGVRRIVFDSFSALYYPPL
                     LIASGDVKKAATYAELARQRSPYEPTYELLLCLAGFHSGKGSDKFFSVFSPARCKKEK
                     YKKGFLFHLALCLSWSSPEALIDPEYRSLFLNALDELRKSGNTFLLYCVELAEMLSLP
                     PEKQIPGRKLPLRDLIPRKEEWMVALGALTTLGKGTGGAKKKRERRILWEVDWDTEEN
                     GTIVSPEFIPVEQVMQSSGKWSKGRQVALKRLQRELVKEPWMTRQDLAAAGAVEEVGG
                     YDGWYYKRWFQFDGVKIFSALAGHPYLVRSGDGRPMEIVQEEPRLDLARTVGGYRVRL
                     SPHPAEGKYANVFLEEETPCRIRVVPFDEKHRRIAEILGKEGLFVPERGKEHILETLE
                     SLAETVPITSEIEGVEGSAVAVEPDSRIVVQLAPGEDGFFVSLAVRPLGTGTHLCRPG
                     EGGKTMMGRSDGQRIQTLRDLDAERRNRDEALVLCPALSEGEQTDENRWELDNPELCL
                     EFLLQLAGMGERVVVEWPKGGAMSVRSALSPNAVKLSVATGRDWFALSGEIRVDEQTV
                     LGIKEACEMLAESKGRFLPLGNGEFLALTAELRKRLEEIGSFGDWRGKELRFSPLSAP
                     LFRKLEKETEGFGGDGEWAKQLALVEEAESIAPALPSTFKGVLRDYQTEGFQWLCRLA
                     HWGAGACLADDMGLGKTIQVLAVLLSRAQTGPSLVIAPVSVCPNWFQEAARFAPTLNL
                     KEFRYGDRQKILDDLEPFDVVVT"
     gene            complement(5084..5929)
                     /locus_tag="P5201_01760"
     CDS             complement(5084..5929)
                     /locus_tag="P5201_01760"
                     /EC_number="1.1.1.262"
                     /inference="COORDINATES: protein motif:HMM:NF016084.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxythreonine-4-phosphate dehydrogenase
                     PdxA"
                     /protein_id="HRV97291.1"
                     /translation="MKMRLRPVEDPADAAGSPESLPVIDLKMVPDLTALSVGKVSALG
                     GKASVAYIQKCVDLCMTGQAAGIATTPINKESLKAAKVHYIGHTEMLAEMSGAPKSYT
                     MFIVDKLRILFHSRHLSLRRAIETLNTDDVVHSIETAAKCLDSIGLPSGTIALAGLNP
                     HSSDGGLFGDEEARILAPAVEEANRRGINVVGPVPADSVFYFGLQGKYDVVVSLYHDQ
                     GHIASKTYDFYRTVSVTFGLPFIRTSVDHGTAFDIAWKGIANPVSMKEAMLACFDIAP
                     RYRPF"
BASE COUNT         1107 a         1837 c         1847 g         1146 t
ORIGIN      
        1 ggtacatcgt tgcgatcaac cgcgacccgg acgcctccat cttccgggtg gcggatctgg
       61 ggcttcgagg ggatcttttc gatattatcc cccggattat tgatcgtatc aaaacggagg
      121 tgaactccag atgagtgttc aagcatactc tccggtcact gaacagacag tagaggaact
      181 ccaaaagctg ataggagcgt cgaacgtctc cgcagacctt gaaaagcgca tcgcctactc
      241 ctacgacgag gtcccgcgtt tttactggga caaggagtat atcgccgagg tcgtcgtttt
      301 cccggagact accgaggatg tctccgcggt gatgcgctac gcgaacgacc gcaggattcc
      361 ggtgacacca agaggggccg gcacaggcct ctccggcggc gcggttcctt tctcgggcgg
      421 gatcgtcctc tcgttcgaga agatgcgggc catcatcgag ctggacaaga aaaacctcac
      481 gatcacggtc gaacctggcg tggtgacggc ggagatcacg aaggcggcgg agcgcgaagg
      541 gctgctctac gccggggacc cgtgcagcgg cgacgcctcc ttcatcggcg gcaacgttgc
      601 ggagaacgcc gggggaaata aggtagtcaa gtacgggacg accggaaact ctgtgctcgc
      661 ccttgaagcg gtgcttccgg acggaacggc gacctggttc gggggcaagc gccggaagga
      721 tgccacgggg tacgacttcg ctcatttgat cgtcggctcc gaaggaacgt tggccgtggt
      781 gacgaagatc attttgaagc tgcttcctct tcccggcaaa gtcctggacc ttctggttcc
      841 ttttcccgac gtggcttccg cgatcgactt cgctcctcgc gtgatgagcg aggggaaggt
      901 gattccgtcg tcgatggagt tcatggacag acgctcgatc aagctggcgg agcgattcct
      961 caacactggc ctccccttcg acgacgcagg ggcgcacctc atcatccagc tcgaagggaa
     1021 cgatccggag gtgcttgccg acgacatgga gcgcatcggg gatctctgct tcgcgcacgg
     1081 cgccttggag gtcttcgtcg cggacaaccg gaccacccga gacaagctct ggaaggcgcg
     1141 aaagtgcctg gcggaggcga tcatggcctt ctacccgaag tacagcctcg aagacctggt
     1201 ggtgcccacc agcgagatac cgaagctgat ggccgagacg acgcgcctct gtacgaaata
     1261 cggcctggag gaggcgaact tcggtcacgt gggggacggc aacatgcacg tgaacattct
     1321 tttccccgag gagcgccccg actggcacag catcgtcgac gaactcctcg acgagctgta
     1381 cgactttacg gcctccatcg gcggcacgct gagcggcgaa cacggtatcg gcctgaaacg
     1441 gcagaagtac atgtgccgcg tgatggacgc ctcgcagatg gagctgatcc acagagtgaa
     1501 gctcgccttc gacccgaaca acatcctcaa cccgggcaag atgatcagct gcccggatcg
     1561 ggagtaggac acaccggaga agcggaacgc aacgggggcc cgagaaggcc cccgttgttc
     1621 attcctgcct gatgagcgcg gccatctcgt tgagcgagaa gctggtcacg cgctccgttc
     1681 cttcgagcag gctttcggcg agatctcgct tcgtcgtgtg aaggtccacg atcttctcct
     1741 cgatggtatc cttcgccacg atgcggtaga ccgtcacggg acgctcctgc ccgatgcggt
     1801 gcgcacggtc ggaggcctga tcctcgacgg cggggttcca ccaggggtcc atgtggacga
     1861 cgtaatcggc ggccgtgagg ttcagccccg ttcctccggc gcgcaggctg atgagaaaac
     1921 actccttctc cccggcctgg aacgcttcca cttggcgttt ccgttcctcg ggcggggtcg
     1981 aaccgtcgag gtatgagtac gaaactccgg cttcgttcag cgatgctcgc aggatggcca
     2041 gatggtcgac gaactggctg aagacaaggc agcggtgtcc gtttgcccga aggtcttcca
     2101 atatctcgcc gaacgcctcc agtttcgcgg aggggaactc tctgtcgggg aggacgagcg
     2161 cgccgttaca gcatgcgcgc cgcagcttca tcagctcggc gaggataacg aagcgctgat
     2221 cctctccgtc tcccttctcg attctttcga gcgcgttcct cctgagcgcc tcgtagaggc
     2281 ttcgctcctc cgggttcata tccacgcgga gggtgatctc cgtcttcgga ggcagctcgt
     2341 cgagcacctg gtccttggtg cggcggagca ggaaggggcg gaggatcttc ttcagccggg
     2401 cgcgcgctcc cttgtcgtcg tacttcacga tcggtgtggc gaagcggcgg ttgaagctgt
     2461 cgagcgagcc gagcagcccc ggattgagaa agcggaagag gttccacagc tcgccgagat
     2521 ggttttcgat cggggtgccg gtggtgataa tcctgaattt ccccttcagc cgcatcgccg
     2581 cggctgagcg cttcgnnnnn nnnnntgtca ccaccacgtc gaacggctcc aggtcgtcga
     2641 ggatcttctg ccggtcgccg tagcggaatt ccttcaggtt cagagtgggc gcaaaacgcg
     2701 cagcctcctg aaaccagttc gggcagacgg agacgggagc gatcacgagc gaaggaccgg
     2761 tctgcgcgcg agagaggagg acggcgagca cctggattgt cttgccaagc cccatatcgt
     2821 ccgcgagaca tgcgcctgcg ccccagtgcg cgaggcggca gagccactgg aatccttcgg
     2881 tctgatagtc gcgcagcacg cctttgaacg tcgagggaag cgcgggagcg atcgactcag
     2941 cttcttcgac gagcgcgagc tgtttggccc actcgccgtc gccgccgaaa ccctccgttt
     3001 ctttctccag cttccggaaa agcggcgccg agagggggga gaagcgcagc tcctttcccc
     3061 gccagtcgcc gaacgagccg atctcttcaa gccgcttccg cagctccgca gtgagcgcga
     3121 gaaactcgcc gttcccgagg gggaggaagc gtcctttgct ttcggcgagc atctcgcacg
     3181 cctctttgat gcccagaacg gtctgttcgt ccacgcgaat ctccccggag agcgcgaacc
     3241 agtcgcgccc cgtagccacg gagagcttta ccgcgttcgg ggagagggcg gagcggacgc
     3301 tcatcgcgcc tcccttgggc cactcgacga cgacgcgctc tcccattcct gcaagctgga
     3361 gcaaaaattc gaggcagagt tcggggttgt caagctccca tctgttctcg tccgtttgtt
     3421 ccccttcgct cagagcaggg cagagcacca acgcctcgtc gcgattgcgc cgctcggcgt
     3481 cgagatcccg gagcgtctgg atgcgttgcc cgtcgctccg gcccatcatc gtcttgccgc
     3541 cctcgcccgg cctgcagagg tgcgtgcccg tcccgagagg gcggacggcg agcgagacga
     3601 aaaaaccatc ttcgccgggg gcgagctgga cgacgatgcg cgagtccggc tccaccgcga
     3661 cggcgctccc ctccacgcct tcgatctcgg aggttatggg aacagtctcc gcaagcgact
     3721 ccaaagtctc cagaatatgc tccttccccc gctccgggac gaagagacct tccttgccga
     3781 ggatctccgc gatgcggcgg tgcttctcgt cgaagggaac cacacgtatc cggcagggag
     3841 tctcctcttc caggaagacg ttggcgtact tgccctcggc agggtgcgga gagagacgga
     3901 cgcggtagcc cccgaccgtt ctcgccaggt cgagtctcgg ctcctcctgc acgatctcca
     3961 tcggcctgcc gtccccgctc cggacaagat acgggtgccc cgcgagcgcc gagaagattt
     4021 tcaccccgtc gaactggaac catcgcttgt agtaccagcc gtcgtagccg ccgacctcct
     4081 cgaccgctcc cgccgctgcg agatcctggc gtgtcatcca cggttccttg acgagctccc
     4141 tctgaagacg cttcaatgcg acctgccgtc ccttgctcca ctttccggag gattgcatca
     4201 cctgttcaac ggggatgaac tccggggaga cgatcgtgcc attctcttcg gtatcccagt
     4261 ccacttccca gagaatgcgc cgctcccgct tttttttcgc tccgccggtt cccttcccga
     4321 gcgtcgtcaa cgccccgagc gccaccatcc actcctcctt gcgggggatg aggtcgcgca
     4381 acgggagttt ccttccgggg atttgcttct ccggcggaag cgagagcatc tcggcaagtt
     4441 cgacgcaata gagcaggaac gtattgccgg acttgcgcag ttcgtcgagg gcgttcaaga
     4501 agagggaacg gtactccgga tcgatcagcg cttccgggga agaccatgag agacagagcg
     4561 cgagatggaa gagaaatccc tttttatatt tttccttttt gcaacgggcg ggagagaata
     4621 cggagaagaa tttgtcgctc cccttgccgg agtggaagcc tgcaaggcag agcagcagtt
     4681 cgtaggttgg ttcgtacggg gaacgctgtc tggcaagttc agcgtacgtc gccgctttct
     4741 tgacgtctcc ggaggcgatc agcagcggcg ggtagtaaag ggcggaaaaa gagtcgaaga
     4801 cgattcgtct gacgccgcgt tccttccgca gacgcttcag gccttcttca aaaagcgcca
     4861 gggcgtcctt gcgccggtcg tcgagaagag cgagcagcgc ccggacagcg tacgattcgc
     4921 ccgacacggc gcccccgatc ttttccgttg cggccgccaa gtctccggag aggatgagcg
     4981 attccgcttg atgtacgaga agctcgtcat tgggaaggga ggagccggag gcccctccct
     5041 tcgtcttctt cagaatatcc ggtatcacgt cgcgcgctcc cggctagaac ggacggtagc
     5101 ggggggcgat gtcgaagcag gccagcatgg cctccttcat gctcaccggg ttggcgatcc
     5161 ccttccatgc gatgtcgaag gcggttccat ggtcgaccga cgtgcggatg aacggcagtc
     5221 cgaaggtgac gctcaccgtc cggtagaagt cgtaggtctt cgaggcgata tgcccctggt
     5281 catgatagag cgagaccacg acgtcgtact ttccctgaag gccgaagtag aagacgctgt
     5341 ccgccggaac cggcccgact acgttaatcc ctctcctatt ggcctcctcg actgccggag
     5401 caaggatgcg cgcctcctcg tcgccgaaga gccccccgtc cgacgagtgc gggttgagcc
     5461 cggcgagagc gatcgtaccc gacggaaggc cgatggagtc gaggcacttc gccgccgtct
     5521 cgatggaatg gaccacatcg tccgtattga gcgtctcaat ggcgcgccgc aacgagagat
     5581 ggcggctgtg gaagaggatg cggagcttgt cgacgatgaa cattgtgtag ctcttcggag
     5641 cgcccgacat ctcggcgagc atctccgtgt gcccgatgta gtgaaccttt gccgctttta
     5701 gcgactcctt gttgatcggg gtggttgcga tgcccgcagc ctgccctgtc atgcagaggt
     5761 cgacgcactt ctgaatgtat gctacggagg ctttgcctcc aagtgcggaa acctttccta
     5821 cggagagagc cgtaagatct ggaaccatct tcaggtcgat caccggaagg ctctccggcg
     5881 atcccgccgc gtccgcagga tcctccacgg gccggagtcg cattttcata cccagcgctt
     5941 ccttcac
//
LOCUS       DAOTIL010000048         5850 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminobacteriaceae bacterium isolate AMR_MDS_3026
            NODE_16191_length_5850_cov_4.723037, whole genome shotgun sequence.
ACCESSION   DAOTIL010000048 DAOTIL010000000
VERSION     DAOTIL010000048.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868050
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminobacteriaceae bacterium (wastewater metagenome)
  ORGANISM  Aminobacteriaceae bacterium
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae.
REFERENCE   1  (bases 1 to 5850)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5850)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:19:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,224
            CDSs (total)                      :: 3,198
            Genes (coding)                    :: 3,184
            CDSs (with protein)               :: 3,184
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5850
                     /organism="Aminobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16191_length_5850_cov_4.723037"
                     /isolate="AMR_MDS_3026"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:3034609"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            125..2485
                     /locus_tag="P5201_01765"
     CDS             125..2485
                     /locus_tag="P5201_01765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006582974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycyl radical protein"
                     /protein_id="HRV97292.1"
                     /translation="MNERIARLRNESFETYPSISIERARLTTDFYRANEGKYPVPVMR
                     ALNFKNLCEQKTLYIGKDELIVGERGPAPKAVSTFPELNCHTARDLEILNTRPMTGYR
                     VHEDDIRIYTEEVVPYWTGRTLRDRIFSNLPEEWLALYEAGCFTEFMEQRAPGHTALD
                     GTIYSTGLLDMKKKIASARERLDWINDPHATEKDEELQAMDIACDAAIIFARRHADLA
                     EEMAASEKDFVRKAELLKIADVCRHVPANAPRDFHEAIQMYWFTHLGTITELNGWDAM
                     SPGHFDLHLRPFYEKGIADGSLDREKAKELLSCFWIKVNNTPAPPKVGVTAAESGTYN
                     DFTQINLGGIAEDGSDGSNDVSYIILDVLDELQLLQPQVSVHISSKTSERFLRHACKV
                     MRRGSGYPSAFNSEAVFMEQLGMGKTVEDARTGGTSGCVETGSFGKEAYILHGYLNVP
                     KIMELALNNGTDPLSGKRIGLETGAPQTFTCYEEFYQAFEKQLHYVVDTKIRTDNYIQ
                     RMFARHAPAPYLSVLIHDCIENGRDYYDGGPRYNTDYIQCCGIGTVTDSFSVLKKHVF
                     EEKTVTLADLLRALHENWEGWEEMRQFIWNRTPFFGNDDEEADSIMQKVYASLLNAID
                     GKHSPKGPTYHVNMLSTTCHIYFGKKLGATPNGRFAHAPESDGTSPSHGADRNGPTAV
                     VKSLSRMDQLKSGGTLLNQRFLPSVLSGDSGLEKMGQLIRTYFKLGGHHIQFNVVDTE
                     TLRKAQKNPDEYRSLLVRVAGYSDYFVDLDQYHQEEIIARTAQENL"
     gene            2617..3633
                     /gene="dctP"
                     /locus_tag="P5201_01770"
     CDS             2617..3633
                     /gene="dctP"
                     /locus_tag="P5201_01770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014163709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter substrate-binding protein DctP"
                     /protein_id="HRV97293.1"
                     /translation="MQKAKKFVTLCILSALVLFLIVPNMASAAPEMTLRFAGQVPLEH
                     TATKLMHQVADEVKEKTNGRIVVEVYPANQLGDYTLVYEELIRGTIDMAAISVPSQFD
                     PRLEVTYINCFVRNFDEAKSVFAPDGWLFKKMDELSARLGVKLLGFQVEGFIGIGSTK
                     PVNEPLNPNVDKGVLVRIPNMDVYKLGAEAMGYRTVTVPWAEVYTALQTGVADGVDGM
                     TPTAAYTMLKDVLKYWYDLNYSVENFNYMISMKTWEKLSEADRKVIADACAKVTEMSI
                     ELAKQDQEKHLQLMRDAGIEVHTYTAEELKPIFEKVSATWGQLSDKFTPELIEEFKKE
                     YAPK"
     gene            3711..4274
                     /locus_tag="P5201_01775"
     CDS             3711..4274
                     /locus_tag="P5201_01775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014163708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0031317 - tripartite ATP-independent
                     periplasmic transporter complex [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015740 - C4-dicarboxylate transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter small permease"
                     /protein_id="HRV97294.1"
                     /translation="MADNETIGCFPENRPEKQSKVDLILLFFFSIVSVSCSLLLSIII
                     SAAAFTRYIIGGDLYGFEEWVRLLAFWLYFMGGAYGAYNDTHVSADVIDSYMKEGFFK
                     RLVVVLRNLITTGISILFTWYGYEFFMFGFMGPLGTGIAIPQTTIWQIPLWTSYLAVF
                     LGLIFMTYYFTMRLFRNLRLLIKGGVS"
     gene            4271..5587
                     /locus_tag="P5201_01780"
     CDS             4271..5587
                     /locus_tag="P5201_01780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006582969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0031317 - tripartite ATP-independent
                     periplasmic transporter complex [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter large permease"
                     /protein_id="HRV97295.1"
                     /translation="MIYIAVLILLVCLVLGVPVPMSFMASTAWLLFFGGPTGAGYASS
                     MVLPYGFSSMNSIALISIALFIMAGGIMEKGRIGEKLIDFVDVFVGRIKGGLGVVGTI
                     SCAVFGSITGSACATLSCIGSIMFPRLEAAGYPRGIAAALMSNASLLGMLIPPNSTLI
                     IFAWISGQSILACFLSTVLPGIMMTALICLVNCWLVRKNPNVKVTEPPKGRARFNLMK
                     QRGFVALPALMLPFIVLGGIYGGIMTPTEAAAVAVIYAIPTGMFIYKGLTLRGLWDSL
                     VESAVTTGVVMVMLYSVMMLSRLYIMENLPGKMLGLFRAVSENPWIILFMINIFLVVM
                     GMLMDDISSVTLGTPILIPIIIELGFNPIHYAAIVGVNTGLGCITPPAAPVLYLGGRL
                     GKAPINEMMGPTLWIIALAWTPTLLITTYFPRFSLLLPHLFLGTPW"
BASE COUNT         1438 a         1551 c         1457 g         1404 t
ORIGIN      
        1 acaaaaacct tggattggag ttcaaaatgg ggcagacgca gcccccgtcg gattatgaag
       61 tttcacaggc gctttcactg tttgcatcgc gggggatcca cgcggtgaaa ggaggataag
      121 agaaatgaac gagagaattg cacgtctgag aaatgagagt ttcgaaacat atccgagtat
      181 ttcaatcgaa cgagcccgtt tgaccaccga tttctacagg gccaatgaag gcaaataccc
      241 ggtgcccgtc atgagggcgc ttaacttcaa aaatctttgt gaacaaaaga cgctctacat
      301 aggaaaagat gagctgatcg tgggggagcg gggccctgct ccaaaggcag tctccacctt
      361 cccggaactg aattgccata cggcaagaga cctcgaaatc ctgaacacgc gtcctatgac
      421 cggatacagg gttcacgaag atgatattcg catctacacg gaagaagtcg tgccctactg
      481 gaccggccgc actctacggg acaggatttt ttcaaacctc cccgaagaat ggcttgcgct
      541 ctatgaagct gggtgtttca ctgaattcat ggagcaacga gccccgggac acacagcgct
      601 cgacggaacg atctattcaa ccggactgct cgacatgaag aaaaaaatcg cctccgctcg
      661 cgaaaggctt gattggatca acgatcctca cgccactgaa aaagacgagg agctacaggc
      721 gatggatatt gcctgcgacg ccgcaattat cttcgcacga cgtcatgccg atctcgccga
      781 ggagatggca gcatccgaga aggacttcgt ccgaaaggca gaactcctca agatcgccga
      841 cgtctgccgt catgttccgg cgaacgctcc gcgcgatttt cacgaggcta tccagatgta
      901 ctggtttaca catctgggca caatcaccga gttgaatggc tgggacgcca tgtccccggg
      961 gcatttcgac ctccatctcc gtccatttta cgagaaggga atcgcagacg gttcccttga
     1021 ccgcgagaaa gcgaaagaac ttctctcctg cttctggatc aaggtcaaca atactccggc
     1081 gcctccgaag gttggcgtta ctgctgcgga gagcggaaca tacaacgact tcacccagat
     1141 caacctgggc ggaatagccg aggacggaag cgacggctca aacgacgtct cctacataat
     1201 actcgacgtt ctcgacgagc ttcagctgct ccagccccag gtcagcgtac acatatcttc
     1261 aaaaacttcc gaacgtttcc ttcgccatgc ctgcaaggtg atgcggcgag gctccggcta
     1321 tccttcggca ttcaactccg aggctgtctt catggagcag ttggggatgg gcaaaaccgt
     1381 cgaagacgcc cgtaccggag gaacaagcgg ttgcgtcgaa accggctctt tcggcaagga
     1441 agcctacata cttcacggat acttgaacgt tccaaagatc atggaactcg ctctaaacaa
     1501 cggaaccgat cctctctccg ggaagcgcat agggttggag acaggcgccc cgcagacttt
     1561 cacctgctac gaggagttct atcaagcgtt cgagaaacag ctgcactatg tagtcgacac
     1621 aaaaatacgt acggataact acatccaaag gatgttcgca cggcatgctc cggctccgta
     1681 cctctccgtc ctcattcacg actgcatcga gaacggcaga gactattacg acggcggtcc
     1741 ccgctataat acggactaca tccagtgctg cggaattggt acggtcacgg atagtttctc
     1801 cgttctcaag aagcatgtct tcgaagaaaa gactgtgacg cttgccgatc ttcttcgcgc
     1861 gcttcacgag aattgggaag gctgggagga gatgcgccag tttatctgga accgcacccc
     1921 gttcttcggt aacgacgacg aagaagccga ctcgattatg cagaaggtgt atgcgtcgct
     1981 tctgaacgcc atcgacggga aacactctcc aaagggacct acataccacg tgaatatgct
     2041 ctccacaacg tgccatatct attttgggaa aaaactgggc gctacaccaa acggacgttt
     2101 tgcccatgcg ccggaatcag acgggacttc gccatctcac ggcgccgaca ggaatggacc
     2161 tacggcggtc gtcaagtccc tcagccgtat ggatcagctc aaatcaggag gcacgctgct
     2221 taatcagcgt ttccttccct cggttctgag cggagactcc gggcttgaga aaatgggaca
     2281 actcatacgg acctacttta aactcggagg acatcatatc cagttcaatg tagtcgatac
     2341 ggagacacta cgcaaggcgc agaagaatcc ggacgagtac cgcagtcttc ttgttcgagt
     2401 ggcagggtat agcgactatt tcgttgacct tgatcaatac caccaggaag aaatcatcgc
     2461 ccgaacagcg caggagaact tataacacat tactccgtgc gcttttttaa ggtgaaggga
     2521 ggggatattc gtccggtgaa aaaataggca taaccatcac gcgacacaag aggcatcatc
     2581 gatgagtacc gcaaaaacga aaggagtgct gacataatgc agaaagcgaa aaaatttgtc
     2641 accttgtgta tcctctccgc ccttgttttg ttcctcatcg ttccgaacat ggcttccgca
     2701 gctcccgaga tgactctgcg cttcgccggg caggtcccct tggagcacac cgccaccaaa
     2761 ctgatgcatc aggttgcgga tgaagtaaaa gaaaagacga acggacgcat cgtcgttgaa
     2821 gtgtatcctg caaaccagct gggcgactac acgcttgtct acgaagagct tatccgcgga
     2881 acgatcgata tggctgccat atccgttccc agccagttcg atcctcgcct tgaagtaacc
     2941 tacatcaact gttttgtcag gaattttgac gaagcgaaat ccgtttttgc tcccgacggc
     3001 tggctcttca agaagatgga cgaactgagc gcgcgccttg gcgttaagct tcttggattc
     3061 caggtcgaag gctttatcgg tatcggcagc accaagccgg tgaacgagcc gctgaatccg
     3121 aacgtggaca agggcgtact cgtccgcatt cccaacatgg acgtctacaa gctcggcgcg
     3181 gaagcaatgg gctaccgtac cgtcaccgtt ccctgggctg aagtctacac agccctgcag
     3241 acaggcgtcg ccgacggcgt tgacgggatg accccgacag ctgcgtacac aatgctcaag
     3301 gatgttctga agtattggta tgacttgaat tactcggtcg aaaacttcaa ctacatgatc
     3361 agcatgaaaa catgggagaa actctcggaa gctgatcgta aggttatcgc cgatgcgtgc
     3421 gcgaaggtca ctgaaatgag catcgaactt gcgaaacaag accaggaaaa gcaccttcag
     3481 cttatgcgcg acgccggcat cgaggtacat acctataccg cagaagagct gaagcccatt
     3541 tttgaaaaag tatccgccac ttggggacag ctttccgata agtttactcc tgaactaatc
     3601 gaagagttca agaaagaata cgcacccaaa tagacaattc aacaaccccg tatcctttcg
     3661 agtagggaca ccccctactc gaaaggatac gagaaggaaa ggagatcttt atggcggaca
     3721 acgagaccat cggctgtttt cctgaaaatc gtcccgaaaa acagagcaaa gtagatttaa
     3781 tcctgctctt ctttttttct atagtttctg tttcctgctc tcttctcttg tctatcatca
     3841 tatctgccgc tgcatttaca cggtatatca tcggcggcga cctctacggc ttcgaggaat
     3901 gggtccgtct gttggccttc tggctttatt ttatgggggg agcatacgga gcttacaacg
     3961 atacacacgt ctccgccgac gttatcgatt cgtatatgaa agaaggtttt tttaagcggc
     4021 ttgtcgttgt tctcaggaac cttataacta ccggcatcag catactcttt acctggtacg
     4081 ggtacgagtt cttcatgttt ggcttcatgg ggcctctcgg caccggaatt gccatccctc
     4141 aaaccactat ctggcaaata cctctgtgga catcctacct ggctgtcttc ctcggcctaa
     4201 ttttcatgac atactacttt acaatgagat tgttcaggaa tctccggctg ctcattaaag
     4261 gaggagtatc atgatttaca ttgctgtact gatccttctt gtttgtcttg tgctgggagt
     4321 tccggtgccg atgagtttca tggcttctac tgcgtggctt ctcttcttcg gcggccccac
     4381 cggcgccggg tacgcatctt ctatggtgct accgtacggt ttcagctcaa tgaacagcat
     4441 cgctctcatc tccatcgccc tctttataat ggcggggggg atcatggaga aaggtcgtat
     4501 tggcgagaaa ctcattgatt tcgtcgatgt cttcgtcgga cgaatcaagg gtggtcttgg
     4561 agtcgtcgga accatctctt gtgcggtttt cggctccatt accggttccg catgtgcaac
     4621 gctttcctgc atcggctcca ttatgttccc cagacttgaa gccgcagggt acccgcgggg
     4681 aattgcagcg gcgctcatgt cgaacgcctc actgctcggg atgcttattc cccccaattc
     4741 aaccctcatc atctttgcat ggataagcgg tcagtcgata ttagcgtgtt tcctttccac
     4801 agtactgccg gggatcatga tgaccgctct tatctgtctc gtgaactgct ggctcgtccg
     4861 taagaacccg aatgtcaagg taaccgaacc tcctaaagga agagcgcgtt tcaatctgat
     4921 gaaacagaga ggcttcgtag ctctacctgc gcttatgctc cctttcatcg tgctcggcgg
     4981 tatttacggc ggtatcatga cgcccaccga agctgctgcc gttgcggtaa tctatgctat
     5041 tccgacgggg atgttcattt ataaaggact gacccttcgc ggactgtggg actcgctcgt
     5101 tgaatcagct gttacgacgg gtgttgtcat ggtcatgctc tattctgtta tgatgctcag
     5161 ccgcctctac ataatggaaa atcttccggg aaagatgctt gggcttttta gggctgtatc
     5221 ggaaaatccc tggatcatcc ttttcatgat taatattttc ctcgtcgtca tgggaatgct
     5281 gatggacgac ataagcagcg ttacgcttgg aacgccgatt ctcattccga tcatcatcga
     5341 acttggattc aacccgattc actacgccgc gatcgtagga gtcaatacag gtttaggctg
     5401 tatcacaccg cctgctgctc cggttctcta ccttggcggg cgtctaggaa aagctcctat
     5461 caatgaaatg atgggaccaa ccttatggat cattgcactt gcatggacgc cgacactgct
     5521 cataacaacc tatttcccga ggttctctct tcttcttccg catcttttcc ttgggacccc
     5581 atggtaaaga acggggaatc cgcgtgacgt acagaaactt ttttggatgt atctgtcttc
     5641 tttgtttctt tacagggctc tggacaaaat caccgtacgc ttattgggga gcgctttttt
     5701 ccggagcgct ctccagagcg tccgtaaaaa aaggttggga gtccccgccc tggatgaaaa
     5761 agctctcgct ttcaacgctg aaaaaaacac ttggactcct tgacaaagac gacaacggtc
     5821 tgtagaatat ctcctcgttg ggtggttagt
//
LOCUS       DAOTIL010000049         5833 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminobacteriaceae bacterium isolate AMR_MDS_3026
            NODE_16253_length_5833_cov_3.691762, whole genome shotgun sequence.
ACCESSION   DAOTIL010000049 DAOTIL010000000
VERSION     DAOTIL010000049.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868050
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminobacteriaceae bacterium (wastewater metagenome)
  ORGANISM  Aminobacteriaceae bacterium
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae.
REFERENCE   1  (bases 1 to 5833)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5833)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:19:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,224
            CDSs (total)                      :: 3,198
            Genes (coding)                    :: 3,184
            CDSs (with protein)               :: 3,184
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5833
                     /organism="Aminobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16253_length_5833_cov_3.691762"
                     /isolate="AMR_MDS_3026"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:3034609"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(135..1082)
                     /locus_tag="P5201_01785"
     CDS             complement(135..1082)
                     /locus_tag="P5201_01785"
                     /inference="COORDINATES: protein motif:HMM:NF016629.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rpn family recombination-promoting
                     nuclease/putative transposase"
                     /protein_id="HRV97296.1"
                     /translation="MGTHDRGYKLLFSNKEMMRDLLLEFVTEEWVDGLDLDTLDRVHD
                     SFVTDDLRERFDDIIWRVSFRGRMVYLCLIVEFQSSPDRWIAGRLMAYIGLLYGDIVK
                     RNEIVDGFFPPVLPIVLYSGEGRWTVPLSLEEMLHPASSVLGRYAPHLSYLLLDESAY
                     SEERLAQSKNLAAFLFRMENSRTPDHLLEIVRSLGGVLDGEEHRHLRRSFAVFVRNAL
                     LPKKTRRRNFPDVEDLVEVERMIAEGTRNWADGWIKEGEERGEKRGEERGEKRGEQRG
                     RERTALRMLEHGMDVALIAKLAELSEERVRQLAAQSSPH"
     gene            complement(1276..1800)
                     /locus_tag="P5201_01790"
     CDS             complement(1276..1800)
                     /locus_tag="P5201_01790"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRV97297.1"
                     /translation="MKKTTIFALVAVLVLVAASGAFAWGGRGGRGMVAPGTEGTTAPM
                     MQRGMAAPGAPGGAAVPMMGRGMAGRTANGRGAMMGNQQMMKGMRGRGASGGMGGRGM
                     MSVWANVEIPQEIRDKQVEMQKLSVDMRAEMQKKPVDRAKVEELYKKRVELRDALSGW
                     RMKTRLDTIEKLQK"
     gene            2041..2691
                     /locus_tag="P5201_01795"
     CDS             2041..2691
                     /locus_tag="P5201_01795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009164815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HRV97298.1"
                     /translation="MLRILLADDHPLTRSGLAAWLEAEEGVELVGQAADGESAWTMLL
                     ETQPDLALLDIEMPNGSGIEIAERIMKRKLPTNVLMLTAYSAQQYVMASVRAGAKGFI
                     LKSAPFDQLRQAIEDTSRGIFYLDPSVSLVSDEAPAEELSEREKEVLILTAQGLPSSE
                     TARLLSITKRTVAAHLTSIYAKLGAKNKTEAILLALKRGVVLLDQLRFIDGKGESL"
     gene            2688..4109
                     /locus_tag="P5201_01800"
     CDS             2688..4109
                     /locus_tag="P5201_01800"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HRV97299.1"
                     /translation="MKKHFLLLLMGTVTLFTAGNFFVSLHAFRTQESAMDSMMRSYVL
                     DLAENFSNTASFTGGRGQAQRHGTPGMMRFRMLSADPALQSPEAGGLLVISRERRIIT
                     ASPGAERLLPLWKGTAPEEEPREVLDAKGESYYVVVRPLDSGNVVLAAVSRTYLLAPL
                     TGVWKTWILSAMATSAVVLVGMFLLWYWLAVPLRGIAERLGNVGWGKERSIRLYNGPI
                     SELEALSSVIERLATESFEKEELKKRYVTDLVQAQESTRGQLARELHDGALQSTVAAV
                     KRIQLAKQSVPSDANSPLRAHLDTAEEVVQTAAGEIRSYCETLSPSWVRLGLSSALEE
                     IAGRLAQAYEGVETDVDIGVELEAAVAALPEDHVLALARIFQEAASNSVRHGGARFIQ
                     VELRKEGDALLFSVEDDGSGFDADAYAGTDFEELRTTGHRGLANINERVRLLRGTMRI
                     MSRPGESCRIDILFPPPSPGETR"
     gene            4204..4839
                     /locus_tag="P5201_01805"
     CDS             4204..4839
                     /locus_tag="P5201_01805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008521650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HRV97300.1"
                     /translation="MVRILLADDHPLTRSGLAAWIAKEEGMQLVAETEDGESAWSAIL
                     ELQPDLAMLDIEMPDCSGIDIASRVLKRGLATNVLILTAYSAHQYVMASVRAGAKGFI
                     LKSAPLAQLRKAIFQTAAGEFYLDPSVSLARTEGDVEQLSERELEVLVLAGQGLPGHE
                     VARLLSITERTVAAHLTSVYGKLGAKNKTEAILLALKRGVILLDQLHLIDS"
     gene            5101..>5833
                     /locus_tag="P5201_01810"
     CDS             5101..>5833
                     /locus_tag="P5201_01810"
                     /inference="COORDINATES: protein motif:HMM:NF024140.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MCP four helix bundle domain-containing protein"
                     /protein_id="HRV97301.1"
                     /translation="MLKRMKIGGKLTLAFGVLLLIFAGVGAMSWMNMREVQREAHALA
                     DEAVPEMVVAASVQQAAQSVMYEIRGYGYTYEPRYLEQGRQKLVEIRNRLKEATDLAA
                     KFPALVRLRENAAKASAAVDQYAALVDRTEAAVQAIAAARTRGDTDQQEFFQLAEAYL
                     ASQNEALEGHIQAGDGADRLRDRAKKINQINGIIDLGNSIQIANFQGQTTRNPALLEE
                     AMKTFDRVDAVLAEIKSTTAQQANLD"
BASE COUNT         1115 a         1966 c         1699 g         1053 t
ORIGIN      
        1 ttggcgatga gcgccacatc cattccgtgc tccagcattc ggagcgccgt acgctcgcgc
       61 cctctctttt cgcctctctc ttcgccttcc ttgatccagc cgtcggccca gttgcagggc
      121 atgctcattt ctcctcagtg aggcgaggac tgcgcagcga gctggcggac gcgttcctcc
      181 gaaagttcgg caagcttggc gatgagcgcc acatccattc cgtgctccag catgcggagc
      241 gccgtacgct cgcgtcctct ctgttcgcct ctcttttcgc ctctctcttc gcccctcttt
      301 tcgcctctct cttcgccttc tttgatccac ccgtcggccc agttgcgcgt tccttcggct
      361 atcattctct ccacctccac caggtcctcc acatcgggaa aattccttct gcgggtcttc
      421 ttcggcagca gcgcgttccg cacgaaaacc gcgaacgagc gcctcagatg cctgtgttcc
      481 tctccatcca gcacgccgcc gagcgaccgc actatctcca gcaggtgatc gggcgtcctg
      541 ctgttctcca tgcgaaaaag aaacgccgcc agattcttcg actgcgcgag ccgctcctcg
      601 ctgtacgcgc tctcgtcgag aagcagatag ctcaaatgcg gagcgtagcg cccgagaacc
      661 gacgaagccg ggtgcagcat ctcctccaaa gagaggggaa cggtccatct cccctccccc
      721 gaatagagca cgatcggcag caccggaggg aaaaagccgt cgacgatctc gttccgtttg
      781 acgatatcgc cgtagagcag accgatatac gccataagcc gcccggcgat ccaccggtcc
      841 ggcgaggact ggaactcgac gatcagacag agatagacca ttctccctct gaagctcacc
      901 ctccagataa tgtcgtcgaa ccgctcccgt aaatcgtcgg tcacgaagga atcgtgcacc
      961 ctgtccagcg tatccaaatc cagaccgtcg acccactcct ccgtgacgaa ttcaaggaga
     1021 aggtcgcgca tcatctcttt attcgaaaag agaagcttgt atcctctgtc gtgagttccc
     1081 atacgaacat tatagaatcg gcggcaagaa tccgcaagcg aagggcgcca tgcttcttaa
     1141 ccgcttcaag agaccgctcg cacgcttgcg caccttggcc gtgccggata cgttcgcaca
     1201 agagagggcg gggaccggag tcctcgccct ctcttcgttg cagcgctgct ttcgggaggc
     1261 gcgctgtttt ttcgcttact tctggagctt ttcgatcgtg tcgagccttg tcttcatccg
     1321 ccagccggaa agggcgtcgc gcagctccac gcgcttcttg tacagctcct cgaccttggc
     1381 gcggtcgacc ggcttcttct gcatttcggc gcgcatatca actgagagtt tctgcatctc
     1441 gacctgcttg tcgcgaatct cctgcgggat ttcgacgttc gcccacacgc tcatcatgcc
     1501 gcgtccgccc atgccgccgc tcgctccgcg accgcgcatc cctttcatca tctgctgatt
     1561 gcccatcatg gcgccccgtc cgttcgcagt acgtccggcc atgcctcttc ccatcatcgg
     1621 aaccgccgcg ccgccgggtg cgccgggagc ggccatacct ctctgcatca tgggcgccgt
     1681 cgttccttcg gttccgggag caaccattcc tctgcctccc cgtccgcccc acgcgaacgc
     1741 tccgcttgcc gctaccagta ccagaaccgc caccaacgcg aatatcgttg tctttttcat
     1801 gagtgaaccc cgcctttcaa aagtgtgttt tgatgtgacg ggtacaggat accgcggcgc
     1861 ggccggacgc gcatggtccg aactaccgct ctcccgactg taacaagtta caatttccga
     1921 agaaacccga agaacgtatt ctttcttcgt tcggttcaaa aaacggatat actataggga
     1981 gagaatcttc acgccgcgcc gcataccgcg gaacgcggct tttgaaaggg ggacgccgag
     2041 atgctgcgaa tactgctcgc cgacgaccat ccgctcacac gctccggcct cgcggcctgg
     2101 ctggaggccg aggagggcgt cgagctcgtc ggacaggccg cggacggaga gtccgcgtgg
     2161 acgatgctgc ttgaaacgca gcccgacctt gcgctgctcg acattgagat gcccaacggc
     2221 agcggcattg agatcgccga gcgcatcatg aagcggaagc tgccgaccaa cgtcctcatg
     2281 ctcaccgcct acagcgccca gcagtacgtg atggcgtcgg tgcgcgccgg ggccaagggg
     2341 ttcattctga agtccgctcc gttcgaccag ctgcgtcagg ccatcgagga cacgtcgcgc
     2401 ggcatcttct acctcgaccc ctccgtctcg ctggtctcgg acgaggctcc cgcggaggag
     2461 ctctccgagc gcgagaagga ggtgctcatc ctcacggcgc aggggcttcc ctcctccgag
     2521 acggcgcgcc tgctctccat caccaagcga acggtcgcgg cacacctgac ctccatctac
     2581 gcgaagctgg gggcgaagaa caagaccgag gccatcctgc tcgccctcaa gaggggcgtc
     2641 gtcctgctgg accagctccg gttcatcgac ggcaagggcg aatccctatg aagaagcact
     2701 tccttctgct gctgatgggg acggtgacgc tcttcaccgc cgggaacttc ttcgtctccc
     2761 tccacgcctt caggacgcag gagtccgcga tggattcgat gatgcgctcg tacgtcctcg
     2821 acctcgccga gaacttctcc aacaccgcct cgttcaccgg aggcagaggc caagcgcagc
     2881 gccacggaac tccggggatg atgcggttcc ggatgctctc cgccgacccc gcgctgcaga
     2941 gccccgaggc gggagggctc ctcgtgatct cgcgcgaaag gcggatcatc accgcgtcgc
     3001 ccggtgcgga acgtctgctc cccctctgga aaggaaccgc acccgaagag gaaccccgcg
     3061 aggtcctcga cgcgaagggc gagagctact acgtcgtcgt gcgaccgctc gacagcggga
     3121 acgtcgtcct cgcggcagtc tcccggacgt acctcctcgc cccgctgacc ggcgtctgga
     3181 agacctggat tctctccgcg atggcaactt cggcagtcgt tctcgtcggc atgttcctcc
     3241 tctggtactg gctcgccgtg ccgctgcggg gaatcgcgga acgcctgggg aacgtcgggt
     3301 gggggaagga acgctccatc cgtctctaca acgggccgat ctccgagctg gaggcgctct
     3361 cctccgtcat cgaacggctc gcaacggagt cgttcgagaa ggaggagctg aaaaaacgct
     3421 acgtcaccga ccttgtgcag gctcaggaga gcacccgggg acagcttgcc cgggagctgc
     3481 acgacggcgc gctgcaatcg accgtggcgg cggtcaagcg catccagctt gcgaaacagt
     3541 ccgtgccttc ggatgcgaac tcccccctcc gcgcgcacct cgacaccgcc gaggaggttg
     3601 tgcagacggc ggcgggagag atccggagct actgcgagac gctctccccc tcctgggtaa
     3661 ggctggggct ctccagcgcg ctcgaggaga tcgccggacg tctcgcgcag gcgtacgaag
     3721 gggtggagac cgacgtggac atcggcgtgg agcttgaagc ggcggtcgcc gcactccccg
     3781 aggaccacgt gctcgcactg gcgcgcatct ttcaggaggc cgcgtcgaac tccgtccggc
     3841 acggcggggc gcgcttcata caggtcgagc ttcgcaagga gggggacgcg ctcctcttct
     3901 ccgtggagga cgacggctcg ggcttcgacg ccgacgcgta cgccggaacc gactttgaag
     3961 agctgcggac gacggggcat cgcggtctgg cgaacatcaa cgagcgcgtc cgcctgctcc
     4021 gcggaacgat gcggatcatg tcacgcccgg gagagagctg ccgcatcgat atcctcttcc
     4081 ctcccccctc gccgggagaa acgcgttgac aggagaaacg gcgttccagc aacagcgtcg
     4141 agcctccgtg gcgtcgaaat gcggcggccg cacacacaat cccgttcaga aaggggcttt
     4201 gccatggtac gtattctgct cgccgacgat caccctctga cccgctccgg cctcgccgcg
     4261 tggatagcga aagaggaggg aatgcagctc gtcgcggaaa ccgaggacgg agaatccgca
     4321 tggtcggcca ttcttgaact gcagcccgac ctggcgatgc tcgacatcga gatgcccgac
     4381 tgcagcggca tcgacatcgc ctcgcgtgtg ctcaagcgcg gactggccac gaacgtcctc
     4441 atcctcacgg cctacagcgc gcaccagtac gtgatggcct cggtcagggc cggcgcaaaa
     4501 gggttcatcc tcaagtccgc cccgctggcg cagctccgca aggcgatctt ccagacggcg
     4561 gcgggcgagt tctacctcga cccctcggtc tcgctggccc ggacggaggg cgacgtcgaa
     4621 cagctctccg aacgcgaact ggaggtgctc gtcctcgccg gacaggggct ccctggccac
     4681 gaggtcgcac gtctgctctc catcaccgaa cggaccgtcg cggcacacct cacatcggtc
     4741 tacggcaagc tcggcgcgaa gaacaagacc gaggccatcc tgctcgccct caagaggggc
     4801 gtcatccttc tggaccagct gcatctgatc gacagctaga gggagactcc cccgcctccc
     4861 cttgcgcgca tccccctctt tccaaaacat ttcccctttc actcagcgaa ttgtcagctg
     4921 taaatcttta cagtcgcaag actgtcgatc tccgtaatgc gtctcccgcc gattccgcgc
     4981 atacttacac cggtgaataa ggctatcgcg aggctctttc tgcgccgacg tccgctggaa
     5041 gagcccgtga aggacggaga gtccgtccgg aaaaccataa aaaaagggag gaatccacat
     5101 atgttgaaga gaatgaagat cggcggaaaa ctcacccttg cgttcggcgt cctgctgctc
     5161 atcttcgcgg gagtcggcgc aatgagctgg atgaacatga gggaagtgca gcgggaggcg
     5221 cacgcgctgg cggacgaggc cgtgcccgaa atggtcgtgg cagcctccgt ccagcaggcg
     5281 gcgcaaagcg tcatgtacga gatacgtggc tacggctaca cctacgaacc gaggtacctt
     5341 gagcaggggc ggcagaaact ggtggagata cgcaacagac tgaaagaggc caccgacctt
     5401 gccgcgaagt tccccgcact cgttcggctt cgggagaacg cggcaaaagc ctccgccgcc
     5461 gtcgatcagt acgcggccct cgtcgaccgc acggaagccg cagttcaggc catcgccgca
     5521 gcgcggacca ggggcgacac ggatcagcaa gagttcttcc aactagccga ggcatacctc
     5581 gcatcccaga acgaggcgtt ggagggacac atccaggccg gggacggcgc ggaccggctt
     5641 cgggaccgcg caaagaaaat caaccagatc aacggcatca tcgacttggg gaactccatc
     5701 cagatcgcca acttccaggg acagaccacg cgaaaccccg ctctcctgga ggaagcgatg
     5761 aagacgttcg accgggtcga cgcagtactg gccgagatca aatcgacgac agcgcagcag
     5821 gccaacctcg acc
//
LOCUS       DAOTIL010000050         5832 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminobacteriaceae bacterium isolate AMR_MDS_3026
            NODE_16261_length_5832_cov_4.682361, whole genome shotgun sequence.
ACCESSION   DAOTIL010000050 DAOTIL010000000
VERSION     DAOTIL010000050.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868050
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminobacteriaceae bacterium (wastewater metagenome)
  ORGANISM  Aminobacteriaceae bacterium
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae.
REFERENCE   1  (bases 1 to 5832)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5832)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:19:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,224
            CDSs (total)                      :: 3,198
            Genes (coding)                    :: 3,184
            CDSs (with protein)               :: 3,184
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5832
                     /organism="Aminobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16261_length_5832_cov_4.682361"
                     /isolate="AMR_MDS_3026"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:3034609"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1267)
                     /gene="mnmG"
                     /locus_tag="P5201_01815"
     CDS             complement(<1..1267)
                     /gene="mnmG"
                     /locus_tag="P5201_01815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014162175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0002098 - tRNA wobble uridine modification
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis enzyme MnmG"
                     /protein_id="HRV97302.1"
                     /translation="MPLYEKKYDVIVVGGGHAGCEAALAAARTGVSVLHLNLYLDNTA
                     LMPCNPSIGGPAKGHLVREGSALGGEQAEAADASAMLVRWLNTSKGPAVRALRAQCDL
                     TEYARHYTMKIETQKNLEVRQETVTDLWIEEGRVRGVRTKFGFDYEAKVVVLATGTYL
                     GGKVHIGPKSFPSGPLGQTPSVELSSSLRAAGLEIRRMRTDTTPRLHADTLDLSSLTS
                     QASADEPLSFSLWGEGTIHEGYFCHLTRSNRRTHEIIRENLHRSPLSMGWMEGSGPRY
                     CPSIEDKVIKFPEKESHLIFLEPVSRSNKEVYVQNFSTSLPFDVQLAMVRSLPGCEHA
                     HITRPGYAIEYDYVVPTQLTPWLETKAVSGLFCAGQINGTSGYEEAAAQGLLAGINAS
                     LKARGEEPLVLGRSESYIGVLVDDLVTKGT"
     gene            complement(1305..2360)
                     /locus_tag="P5201_01820"
     CDS             complement(1305..2360)
                     /locus_tag="P5201_01820"
                     /inference="COORDINATES: protein motif:HMM:NF024867.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HRV97303.1"
                     /translation="MRCGALRLRNFKNITTETILWGEGLNLLVGKNGAGKTNCLEALH
                     LLLGWGPLGDRKDIASWGSAEKKTYVTGDFFGEEEAFLAVGIGGSTVVKYNGKRCSFP
                     EVRSVAPSLAFLPSDMELLDGSPARRRSFLDRLCALLWPLYARRLVEFRRAVRHRIVL
                     LRQGGNLVALSKAMAPLASWLWGARASAVSALAEGLAALPDLLPLPVRLAHVRGGAGR
                     VKDPLEDWWESLEARREKERLCCSPLVGPHRDDLEVCCGDRSAASCLSRGQKRRASAA
                     LMIAAGRCVERRLRRSPILLLDEIASELDAAGREVTVETLRSTGWQVVATAAGEVVPD
                     WPGLTLRVEGGAVLPCT"
     gene            complement(2370..3548)
                     /gene="dnaN"
                     /locus_tag="P5201_01825"
     CDS             complement(2370..3548)
                     /gene="dnaN"
                     /locus_tag="P5201_01825"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013047417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit beta"
                     /protein_id="HRV97304.1"
                     /translation="MRLQISKGEFMKNWQIAERSTSSKSTINSLTGVLLRTADADNSV
                     LMEATDLKTSIKCISQGVLVEEEGEAVLPVKLVGELFKKAPTNVFTVSITDGKGIIIA
                     GRNKYKFTTYSPREFPKLPVSESAAFFCTATASELLRAVSEGTVACTTGEEFPKYLGT
                     AFFQLRDGEMRVVATDGRRLSLSKCSPVRTGENCEFLLPITGVRELQRLLGALDGESP
                     IEILVESTLVFFQMGSMEFSVRRVDTAFPNYENILNPQTTTTMDIDRHELIAALERVD
                     VIVRDFSHMVLVRLSPEGDLLLTGKAPETGAVQEVLDAKIEGESLTVAFNVSFLIEGL
                     KALYGDRVFISFNGPDGQMTMLRPGEKDFLYMAMPIKINESDLLHGEMDEENETEIPE
                     "
     gene            complement(3776..5104)
                     /gene="dnaA"
                     /locus_tag="P5201_01830"
     CDS             complement(3776..5104)
                     /gene="dnaA"
                     /locus_tag="P5201_01830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008519867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003688 - DNA replication origin binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA];
                     GO_process: GO:0006275 - regulation of DNA replication
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromosomal replication initiator protein DnaA"
                     /protein_id="HRV97305.1"
                     /translation="MVDNDLQHLWDHILDEAGSQLPAGATETWLRTCAPTEFTDGVLV
                     LDVPNVFVKEQISSRFLKELIHIFTETGGGRSIELRVGAETRKEERERAERAVSAGNL
                     ESRSGLNPGYLFNSFVVGKSNRLAHAASLAVAETPGEAYNPLFIWGGVGLGKTHLMHA
                     IGHYVLDRNSNSRVTYVSSEKFINEFIQSIKNNKTQEFKSKYRSVDILLIDDIQFLGN
                     KGSSQEEFFHTFNQLHTSKKQIIICSDRPPKEIQSIEDRLVSRFEWGLVTDIQSPDLE
                     TRIAILQKKAQLRRYNIPDDVINFLAINIPSNIRELEGALNRVVACAELAAEPINEEN
                     TREWLKDVIRKDLRGPVSIDSIQNTVADFFSMSVDELLSSKRTSDLAMARQIAMYLCR
                     KITESSLQQIGMAFKKKDHTTVLHAHRKIAQMIKEQPRIKQIVDTIEGKL"
     gene            5611..>5832
                     /locus_tag="P5201_01835"
     CDS             5611..>5832
                     /locus_tag="P5201_01835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008708842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphohydrolase"
                     /protein_id="HRV97306.1"
                     /translation="MDHSLLHIISHTDLDGIAAAAVAWHRWQPTRPLKVSLAGYGTVD
                     ALILESIEAGQEFVVIDLFCQNEKTIEKLD"
BASE COUNT         1107 a         1814 c         1700 g         1211 t
ORIGIN      
        1 tcgtcccctt cgtgaccagg tcgtccacga gcacgccgat gtacgactcc gaccggccga
       61 gcacgagcgg ctcctccccc cgcgccttca gcgacgcgtt gatccccgcg agcagcccct
      121 gcgccgcggc ctcctcgtac ccggacgttc cgttgatctg cccggcgcag aagagtccgg
      181 agacagcctt cgtctccagc cagggagtga gctgcgtcgg cacgacgtag tcgtactcga
      241 tggcgtagcc cgggcgggtg atgtgcgcat gctcgcaccc cgggagcgag cggaccatcg
      301 caagctggac gtcgaacggc aggctcgtcg aaaagttctg gacgtacacc tctttattgc
      361 tccgcgagac cggctccaga aagatcaggt ggctctcctt ctccgggaat ttaatgacct
      421 tgtcctcgat ggacgggcag tagcggggtc cgctcccctc catccacccc atcgaaaggg
      481 gcgagcggtg caggttctcc ctgatgatct cgtgcgtgcg gcggttgctc ctcgtcaggt
      541 ggcagaagta cccttcgtgg atcgtcccct ccccccagag ggagaaggag agcggctcgt
      601 cggcgctcgc ctgcgaggtc agcgacgaga ggtcgagcgt gtcggcgtgc aggcgcggcg
      661 tcgtgtccgt gcgcatcctg cggatttcga gcccggctgc gcgaagcgac gacgacagct
      721 ccacggaggg cgtctgtccg agaggaccgg acggaaagct cttcggcccg atgtgcacct
      781 tgccgccgag gtaggttccc gtggcaagca cgacaacctt cgcctcgtag tcgaagccga
      841 acttcgtccg gacgccgcgc acgcgcccct cctcgatcca gaggtcggtc acggtctcct
      901 gccgcacttc gaggttcttc tgcgtctcta tcttcatcgt gtagtgcctg gcgtactcgg
      961 tcaggtcgca ctgcgcgcgc agcgccctca ccgcgggccc cttcgacgtg ttgagccagc
     1021 ggacgagcat cgcggaggcg tcggccgcct cggcctgctc gccgccgagc gcgctcccct
     1081 cgcggacgag gtgacccttc gcgggaccgc ctatcgacgg gttgcacggc atcagggcgg
     1141 tgttgtccag gtagaggttg aggtggagga cggagactcc cgtccgcgcg gcggcgaggg
     1201 ccgcctcgca ccctgcgtgc cctcctccga cgacgatcac gtcgtacttt ttctcataca
     1261 gcggcatctt ctcgatcaca atcctttgaa ctcaacttga acggctatgt acatgggagg
     1321 acggcgcccc cctcgacgcg cagggtcagt ccgggccagt ccggaacgac ctctcccgcg
     1381 gccgtggcga ccacctgcca ccccgttgat ctcagcgttt ccacggtgac ttcgcgcccg
     1441 gcggcgtcga gttcggaggc gatctcgtcg agcagcagaa tcggcgaacg gcggaggcgg
     1501 cgctcgacgc accggcccgc cgcgatcatc agcgccgcgg aggcacgccg cttctgtccc
     1561 ctgctcagac aggaggcggc ggaacgatcg ccgcaacaaa cctccagatc gtccctgtgc
     1621 ggcccgacaa gcggcgaaca gcagagacgc tccttctccc tccgcgcttc gagggactcc
     1681 caccagtcct caagagggtc cttcaccctc cccgcgccgc ctctgacgtg cgccagccga
     1741 accggcagcg gcagaagatc aggaagcgcc gcaagtccct cggcaagcgc cgaaacggcg
     1801 gacgctctcg ccccccagag ccacgaggcc agcggcgcca tcgccttgga gagcgcgacg
     1861 aggtttcctc cctgccggag cagcacgatc ctgtgccgca ccgcccgccg gaactcgacg
     1921 agacgccgcg catagagcgg ccagagcagc gcgcacagac ggtcgagaaa cgaacgcctc
     1981 cgggcgggag atccgtcgag cagctccatg tcggaaggaa gaaacgcgag cgacggagcg
     2041 acggaacgga cttcggggaa ggagcagcgt ttcccattgt acttgacgac ggtgcttcct
     2101 ccgatgccga cggcgagaaa cgcctcctcc tcgccgaaga agtcgccggt gacgtacgtc
     2161 tttttctcgg cgctgcccca ggaagcaata tctttcctgt cgcccagcgg tccccagccg
     2221 agcagcagat gaagcgcctc aagacagttc gtcttcccgg cgccgttctt cccgacgagc
     2281 agattcaatc cttcgcccca cagaatcgtc tccgtcgtta tattcttgaa gtttcgaaga
     2341 cgaagcgccc cgcagcgcat tcagctcccc tattcgggaa tctccgtctc gttttcctcg
     2401 tccatttcgc cgtggagtaa atcgctctcg ttaatcttga tcggcatcgc catgtagagg
     2461 aagtccttct cccccggtct gagcatggtc atctgtccgt ccgggccgtt gaagctgatg
     2521 aagacgcggt cgccatacag ggctttcagc ccttcgatca ggaaagagac gttgaaggcc
     2581 acggtcagcg attctccctc gatcttcgcg tcgagaacct cctgaacggc tccggtctcg
     2641 ggcgccttcc ccgtaaggag gagatcgcct tccggcgaaa gacggacgag caccatgtga
     2701 ctgaaatcgc gcacgataac gtccacgcgt tcgagcgcgg cgatcagttc gtgcctgtcg
     2761 atatccatcg tcgttgtcgt ctgtggattc agtatgtttt cataattcgg gaatgcggta
     2821 tccactctgc ggacggagaa ctccatagag cccatctgaa agaacacgag cgtgctttca
     2881 acaaggatct cgatgggaga ctcgccgtca agcgcgccaa gaagccgttg cagctcgcgg
     2941 actcccgtga tgggaaggag aaattcacag ttttctccgg tgcgtacggg ggaacacttt
     3001 gacagggaga gacgtctgcc gtccgtggcg acgacgcgca tttctccgtc tcgaagctgg
     3061 aaaaaagccg ttcccaggta cttcgggaac tcctcgcccg tggtgcaggc aacggttccc
     3121 tcggagaccg cgcgaagcag ctccgacgcc gtcgccgtac agaaaaaggc cgcgctttcg
     3181 gagaccggaa gcttgggaaa ctcacgggga gagtacgtgg tgaacttgta cttgttccgc
     3241 cccgcgatga tgatcccctt gccgtcggtg atggagacgg tgaagacgtt cgtcggcgct
     3301 ttcttgaaga gctcgccgac gagtttgacc ggaagaacgg cttctccctc ctcttcgacg
     3361 agaacgccct gcgagatgca cttgatcgag gtcttcagat ccgtagcctc cataagtacg
     3421 ctgttgtccg cgtcggcagt ccgcagtaaa actcctgtga gggaattgat cgtactttta
     3481 gagcttgtgc tcctttcggc gatctgccag tttttcatga attctccctt gctgatttgc
     3541 agtctcatgc ctttccctcc cgggtctcat cttttattgt tctttatctt ttagtagcag
     3601 taatggtaat aaggggtgtg cgaattgtgg ataaccgtcc ccggaccttt ggaagacgaa
     3661 cgacttcact gtggacagac ctgggcgatt cattcagaaa ttatccacat tgtccacagg
     3721 acattccgta catttcttca ttctgcgaga gttatccaca tatcgacccc gagtttcaca
     3781 gcttgccctc tattgtatcc acaatctgtt tgatccgcgg ctgctctttg atcatctgcg
     3841 ctatctttct gtgcgcgtgg agcaccgtcg tgtggtcctt cttcttgaac gccatgccta
     3901 tctgctgcag gctcgactcc gtgatcttcc ggcagaggta catcgcgatc tggcgggcca
     3961 tcgcgagatc ggacgtcctc ttcgagctga gcagttcgtc caccgacatg ctgaagaaat
     4021 cggcgacggt attctgaatg gagtcgatgc tcacagggcc gcgcaggtct tttctgatga
     4081 cgtccttcag ccactcccgc gtgttctcct cgttgatggg ctcggcggcg agttctgcgc
     4141 aggccacgac gcggttgagc gcgccctcga gctcgcggat gttgctcggg atgttgatcg
     4201 cgaggaagtt gatcacgtcg tccgggatgt tgtagcggcg gagctgcgcc ttcttctgga
     4261 ggatcgcgat gcgcgtctcc aggtcgggcg actggatgtc ggtcaccagc ccccattcga
     4321 aacggctcac aagacggtcc tcgatgctct ggatctcctt cggaggccgg tcggagcaga
     4381 tgatgatctg cttcttcgat gtgtggagct ggttgaacgt gtggaaaaac tcctcctggc
     4441 tgcttccctt gttcccgagg aactggatgt cgtcgatgag cagaatgtcc acgctccggt
     4501 acttcgactt gaactcctgc gtcttgttgt tcttgatcga ctggatgaac tcgttgatga
     4561 acttctccga gctgacgtac gtcacgcgcg agttcgagtt ccggtcgagc acgtagtgcc
     4621 cgatcgcgtg catgagatgc gtcttcccca aaccgacgcc cccccagatg aagagcgggt
     4681 tgtaggcctc tcccggcgtt tcggccacgg cgaggcttgc ggcgtgcgcc agccggttcg
     4741 acttccccac gacgaacgag ttgaagaggt accccgggtt caaaccgctg cgggactcca
     4801 aattcccggc ggagacagcg cgttcggccc gttcccgctc ttccttccgg gtttcggcgc
     4861 ccaccctgag ttcgatcgac cgtcccccgc ccgtctccgt aaagatgtgg ataagctcct
     4921 tcagaaaccg ggaggatatc tgctccttta cgaagacatt ggggacgtcg aggacgagca
     4981 cgccgtccgt aaactcggtg ggagcgcagg ttctgagcca cgtttccgtc gcccccgcag
     5041 ggagttgcga accggcctcg tcgagaatat gatcccacag atgctgtaaa tcgttgtcca
     5101 caatacttca cctcatgctg agaatggcac catattatca cagagactgt ggataagctg
     5161 cacattcgcg cctgaaacgg gaaaaagccg ccctttcaat ccattgtcgc cttggaaaga
     5221 gatacgttgt ccacaaaagt tatccacata tccacaaaat gagtttcgtt ttgtggatag
     5281 ggcgaatggc ggatgtggac ggatgtaaag aagttgttac aaaattcgcg gtctgtccac
     5341 aaactcctct gcgcttgcag gaagcaaaat gagctcttta tccacaaact ttccaccgtt
     5401 gtggataaag ggtgtggatg cctgtgaatc tacgaattac tgtttctcca ggtgaaccgg
     5461 ggcgggaaaa agagacgccg ccgtccgtcc ggaaattcag ttgcccgaca gtttcaaaga
     5521 gcggacggaa agggtagaat acagtgcgga gtcatttttt caacactctc gcaggacaac
     5581 accctgttcc gccaaaaagg agaataccat atggatcact cgctgcttca catcatcagc
     5641 cacaccgacc tcgacggcat cgccgccgcc gccgtcgcct ggcatcgctg gcagccgacg
     5701 cggccgctga aggtctcgct ggccgggtac ggcacggtgg atgcgctcat cctcgaaagt
     5761 atcgaggccg gccaggagtt cgtcgtcatc gatcttttct gccagaacga gaagacaata
     5821 gaaaaactgg ac
//
LOCUS       DAOTIL010000051         5812 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminobacteriaceae bacterium isolate AMR_MDS_3026
            NODE_16338_length_5812_cov_4.313531, whole genome shotgun sequence.
ACCESSION   DAOTIL010000051 DAOTIL010000000
VERSION     DAOTIL010000051.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868050
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminobacteriaceae bacterium (wastewater metagenome)
  ORGANISM  Aminobacteriaceae bacterium
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae.
REFERENCE   1  (bases 1 to 5812)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5812)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:19:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,224
            CDSs (total)                      :: 3,198
            Genes (coding)                    :: 3,184
            CDSs (with protein)               :: 3,184
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5812
                     /organism="Aminobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16338_length_5812_cov_4.313531"
                     /isolate="AMR_MDS_3026"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:3034609"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..419
                     /locus_tag="P5201_01840"
     CDS             <1..419
                     /locus_tag="P5201_01840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009201171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="4-hydroxy-3-methylbut-2-enyl diphosphate
                     reductase"
                     /protein_id="HRV97307.1"
                     /translation="LGVISQTTQKGEFLGSAASILAPQIRELRVFNTICGATTQRQEA
                     VREITSRVDGLLVVGGRNSANTAKLVEIARSRNCDVLWIEHAGELSGEWFSGKHKIGI
                     AAGASTPDWLIEEVKHAIETSQVSRGMEGYDGRNDE"
     gene            397..1959
                     /locus_tag="P5201_01845"
     CDS             397..1959
                     /locus_tag="P5201_01845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013048410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S1 RNA-binding domain-containing protein"
                     /protein_id="HRV97308.1"
                     /translation="MTEEMMNEGLRTTDVENAEGREVEETGEAQETMESILEQYGEVE
                     ELHRGKIVTGTVVNVVDGGWLVDVGYKCEGFLPAKEWSHRVLVEDEAEPAIEEKIQIQ
                     VVSIRHGEEAQLLVSRWRCEFDRRWSELESKLAQNEVIPVKGIRKVKGGLMVECCGLE
                     GFVPISHLAEEGRGVNPGKFVGEVFDAKLMEKDKKKHRLVFSRRMIVEETLVAERESF
                     YTDVAEGSVLDGEVSSITSFGVFVNVGPIDGLVHMSELSWKRNIKPKEMFKKGDPVKV
                     RVIGIDRENNRVSLSIKQTENDPWTTASSKWKKNEVTKGVVTNVTDFGAFVEVEPGIE
                     GLIHIGDLSWTRIKHPRDVLRKGQEVEVQVLDVDMEKKRMSLGFKQLNDPWNDIATRY
                     SKDQDLTVKVVRLADFGAFVEIEEGVEGLIHISQLSTKRVEKPGDVLSEGQEVLARII
                     EINPSDRRMRLSISALEEPAGESSARPRDEERRRGKQERGDHDRHPQKNVMSDGEANV
                     TIGEFLKNSLQD"
     gene            2027..2545
                     /gene="def"
                     /locus_tag="P5201_01850"
     CDS             2027..2545
                     /gene="def"
                     /locus_tag="P5201_01850"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005662186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042586 - peptide deformylase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HRV97309.1"
                     /translation="MNSLRLRVYPDPVLRKETEPVVAFDDDLKDLVAEMIRLMNLHDG
                     VGLAAPQAGVSSRLAVVFHDGMLHVLANPVLLSSEGEQDGEEGCLSFPGIFGNVHRPS
                     RITIRHNDLDGVEHETSVEGFLARAFLHEMDHLNGRLLIDNFSPMRRAMARKKLLRSK
                     EGEDAGGGSSRR"
     gene            2542..3489
                     /gene="fmt"
                     /locus_tag="P5201_01855"
     CDS             2542..3489
                     /gene="fmt"
                     /locus_tag="P5201_01855"
                     /EC_number="2.1.2.9"
                     /inference="COORDINATES: protein motif:HMM:TIGR00460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004479 - methionyl-tRNA formyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006431 - methionyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionyl-tRNA formyltransferase"
                     /protein_id="HRV97310.1"
                     /translation="MKDAFQRNPVLWFFGTGTFAASCLKHLVKNVHFDCIVTSPPSSG
                     GRGMRNILSPVETLCETLHLHVIRSSKVSEEPELLHHLAESPPDCILVVDFGQKILEP
                     FLSAPAWGCLNIHPSLLPSYRGAAPVPRALLNGDAVTGVSLFRLVEEMDAGPILFRKE
                     LTVGETASAGEILEILAEKGSELYLLGVKCLIEGSCKFRDQNSESATYAPKIANKEAE
                     CSWVWPSHRLVSTVRAMNPSPGAYIFIGAKRMKIWEVLHHPSLGRPGEIVDFLDGFPL
                     VGTSDGSVALLCVQPEGKKRLSGAEWARGIRLKKGDFLQ"
     gene            3486..4274
                     /locus_tag="P5201_01860"
     CDS             3486..4274
                     /locus_tag="P5201_01860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013048407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRV97311.1"
                     /translation="MNASEREQISQLMASFQTFASTLEKWPKAVAVTGALGSGKTEWV
                     LNLALGFEAIGDQVTIADLDIINPYFCIRQVTDALGARGFNIIMPPENARWSDMTVVS
                     PKISSALATDEGRLLLDIGGDAEGALALKQFEPDIRRAGYLLILVINAFRPQTSSVRG
                     VRTMLERMESICGLSVGALISNSHLMDETTAEHCADGLETVLEAGSELGLPVLYAGVP
                     PKLFPEAASVWKSRNLSVPCWPLSRYMMLPWEEGAMWSRPSTGE"
     gene            4313..4531
                     /locus_tag="P5201_01865"
     CDS             4313..4531
                     /locus_tag="P5201_01865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008521982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HRV97312.1"
                     /translation="MAKKGRIEVFEQFCKSCGLCVAACPVKVLRISEKTNAKGYRPVE
                     QFKEGCIGCGMCAMTCPDAAIEVFKIEE"
     gene            4558..5619
                     /gene="vorB"
                     /locus_tag="P5201_01870"
     CDS             4558..5619
                     /gene="vorB"
                     /locus_tag="P5201_01870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013048405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-methyl-2-oxobutanoate dehydrogenase subunit
                     VorB"
                     /protein_id="HRV97313.1"
                     /translation="MGRVLMKGTEAIAEAAIQAGCKYFFGYPITPQNEIPEYMSAHLP
                     EVGGVYVQAESEVASINMVLGGGATGYRVMTSSSSPGISLMSEGISYVAGCEIPAVIV
                     NVMRAGPGLGGILPGQADYLQSTKGGGNGDYNLIVLAPSTLQECVDLTQLAFDLAQKY
                     RNPVMVAADGFMGQMMEAVEIKPSPSKDQPADPSWALGFMGERGGKRSHLHSLFLSPE
                     PLEEHNRKLQKKYQAMKDAEQRCECYMMDDAEVLIAAYGTTARISRSAVNNLRAEGLK
                     VGMIRPITLWPYPYEAFKKLPSTVKHILVSEMNAGQMVDDVKVATGCKYPVSFYGRMG
                     GFAPSVGEIENECRRILGL"
     gene            5635..>5812
                     /locus_tag="P5201_01875"
     CDS             5635..>5812
                     /locus_tag="P5201_01875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013048404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoglutarate oxidoreductase"
                     /protein_id="HRV97314.1"
                     /translation="MSEVKVYERPKSWMPNVSSHYCPGCGHGIAHRLVCEAIDELGIQ
                     NDTIGVAPVGCAAMI"
BASE COUNT         1325 a         1496 c         1776 g         1215 t
ORIGIN      
        1 ggttgggagt aatatctcag acgacccaga agggcgagtt cctcggatcc gccgcctcca
       61 tacttgcgcc gcaaatccgc gagttgcggg tgttcaacac gatatgcggc gccacgacgc
      121 agcgtcagga ggccgtgcgg gagattacct cgcgggtcga cggactgctc gtggtcggag
      181 gccgaaacag cgccaacacg gcgaagctcg tcgaaatagc ccggtcgcgc aactgcgacg
      241 tcctgtggat tgaacatgca ggggagcttt ccggcgagtg gttctcaggg aaacacaaaa
      301 ttggaatagc agccggcgcc agcacaccgg actggttgat agaagaagtg aaacacgcaa
      361 tagagacatc gcaggtgtca agggggatgg aaggttatga cggaagaaat gatgaatgaa
      421 ggactccgta caacggacgt cgaaaacgcc gaagggcgcg aagttgagga aacgggcgaa
      481 gcacaggaga cgatggagag catcctcgaa cagtatggag aggtcgagga gcttcatagg
      541 ggaaagattg tcaccggtac agtggtgaac gtcgtcgacg gcggctggct tgtggacgta
      601 ggatacaagt gcgagggctt ccttcccgca aaggaatggt cgcacagggt tctggtcgag
      661 gatgaagcgg agcccgcaat cgaagagaaa atccagatcc aggtggtgag cattcgccac
      721 ggcgaagagg cgcagcttct tgtcagccgt tggagatgcg agttcgaccg ccgctggagc
      781 gaacttgaaa gcaaacttgc ccagaacgaa gtcattccag tgaaggggat ccgcaaggtc
      841 aagggcggtc tcatggtgga gtgctgcgga cttgaggggt tcgtccccat ttcgcatctt
      901 gccgaagagg gcagaggcgt caatcccggc aaattcgtgg gcgaggtctt cgacgctaaa
      961 ttgatggaaa aggacaagaa gaagcaccgg ctcgtcttct ccagacgtat gatcgtcgag
     1021 gagacgctgg ttgcagagcg cgagtctttc tacaccgacg tggcggaagg ctcggttctt
     1081 gacggagaag taagcagcat cacatctttc ggcgtcttcg tgaacgtcgg tcccatcgac
     1141 ggactcgtcc acatgagcga gctttcctgg aagcggaaca tcaagccgaa ggagatgttc
     1201 aagaagggcg acccggtcaa ggtgcgcgtc atcgggatcg accgcgagaa caaccgcgtt
     1261 tctctcagca tcaagcagac tgagaacgat ccgtggacca cggcttcttc caagtggaag
     1321 aagaatgagg tcaccaaggg agttgtcaca aacgtgaccg acttcggcgc attcgtcgag
     1381 gtcgagcccg gaatcgaagg cctcatccac atcggcgatc ttagctggac ccgcataaag
     1441 catccgagag atgtgcttcg caaggggcag gaggtcgagg tgcaggtgct cgacgtcgac
     1501 atggagaaga agcgtatgag cctcggcttc aagcagctga acgacccgtg gaacgatatt
     1561 gccaccaggt attcaaagga tcaggatctg accgtcaagg ttgtccggct tgctgatttc
     1621 ggcgctttcg tcgagatcga agagggcgtc gaggggctga tccacatctc ccagctgagc
     1681 acaaagaggg ttgagaaacc gggcgacgtg ctctccgagg ggcaggaagt tctcgcccgt
     1741 atcatcgaga tcaatccttc ggaccgcaga atgcgcctga gcatcagcgc acttgaagag
     1801 ccggccggag agtcttccgc ccgcccccgc gacgaggaac gccgcagagg gaagcaggaa
     1861 cgcggcgatc acgaccgtca cccgcagaag aacgtgatga gcgacggcga ggcgaacgtc
     1921 accatcggag agtttttgaa gaactccttg caggactaac gtacgtcaaa gggtcgcctt
     1981 aaaaaaggcg gcccttttta tttacaaaga aagtgcggcg aatataatga attcattgcg
     2041 gcttcgtgtg tatcccgacc cggttctgag gaaagagaca gagccggtcg ttgcttttga
     2101 cgatgacctg aaagaccttg tcgccgagat gatccggctt atgaatctgc acgacggggt
     2161 aggattggcg gctccgcaag ccggagtttc ttcccggctc gccgttgtgt ttcacgacgg
     2221 aatgctccat gttctggcca atccggtgct gctctcttcg gaaggagaac aggacgggga
     2281 agaaggatgc ctgagttttc cggggatttt cggcaatgtc caccggcctt ccaggatcac
     2341 catacggcac aacgacctcg acggggttga acacgagacg tctgtggagg gctttcttgc
     2401 ccgcgcgttt ctacacgaaa tggaccatct gaacgggagg ctgctcatcg ataacttctc
     2461 tccaatgagg cgcgccatgg cgcgaaaaaa gttattgcgt tcaaaagaag gggaagacgc
     2521 cggcggaggt tcgagcagac gatgaaggac gccttccaaa gaaaccccgt cctctggttt
     2581 ttcggaaccg ggacgtttgc ggcgagctgc ctgaagcatc tcgtaaagaa cgttcatttc
     2641 gattgtatcg tgacctcgcc gccctcttca gggggaagag gtatgcgcaa catcctctct
     2701 cctgttgaga cactctgcga aacacttcac ctccatgtga tccgttcctc caaggtttca
     2761 gaagagccgg aactgcttca tcacctcgca gagtcgcctc cggactgtat tcttgttgtg
     2821 gatttcgggc agaagatact ggagcccttt ctctcggcgc ctgcgtgggg gtgtttgaac
     2881 atccacccgt cgttgcttcc ttcatacaga ggggctgccc ctgtgccgag ggcgcttctg
     2941 aacggggacg ctgtaaccgg agtttctctc ttccgtctcg tagaggagat ggatgcgggg
     3001 ccaatcctct ttcgaaaaga gttgacggta ggcgaaaccg caagcgctgg agaaattttg
     3061 gagattcttg cagaaaaagg tagcgaactt tatctgttgg gtgtaaaatg tcttattgag
     3121 ggaagttgca aatttagaga tcaaaattca gaatcagcaa catatgcacc taaaattgcc
     3181 aacaaagagg ctgaatgctc ttgggtctgg ccctcgcatc gtcttgtctc caccgttcgg
     3241 gcgatgaacc cctctccggg cgcttatatt tttattggcg caaaacggat gaaaatttgg
     3301 gaagtcctcc atcatccatc cctggggcgc cccggcgaaa tcgtagactt tctcgacgga
     3361 tttcctcttg ttggtacgtc agacgggtct gtcgcgcttc tttgtgttca gcctgagggg
     3421 aaaaaacgcc tcagcggcgc ggaatgggcc cgcggaatcc gcctgaagaa aggggatttt
     3481 cttcaatgaa cgcctctgaa cgcgaacaaa tcagccagtt gatggcgtcc tttcaaacat
     3541 ttgcctccac tcttgaaaaa tggccgaagg cggttgccgt aacaggcgcg ctcggctccg
     3601 gaaagaccga atgggtcctc aatctcgctt taggttttga agcgatcggc gatcaggtta
     3661 cgattgccga cctggatatt atcaacccct atttttgtat ccgccaggta acggatgcgc
     3721 tgggcgcaag agggttcaat atcatcatgc cccctgaaaa cgccaggtgg tccgacatga
     3781 ccgtcgtgtc gccgaaaatc tcaagcgcgc tcgcaaccga cgaggggcgt ctgcttctgg
     3841 atatcggagg ggacgcggag ggcgcgctgg cgctgaagca gttcgagccg gacatccgcc
     3901 gcgcggggta tcttttaatc ctcgttatca acgctttccg tccgcagacg tcttcagtac
     3961 gcggtgtgcg gacaatgctg gaaaggatgg agagtatctg cggcctttca gtgggggcgc
     4021 ttatcagcaa ctctcatctc atggacgaga cgacggcgga acattgtgcc gacggacttg
     4081 aaacagttct ggaagccgga agcgaactcg gtcttcccgt gctgtacgcg ggcgttcctc
     4141 cgaaactgtt tcccgaggcc gcttccgtgt ggaaatcccg gaatctttcc gttccctgtt
     4201 ggccgctctc ccggtacatg atgcttccgt gggaggaagg ggccatgtgg tccaggccgt
     4261 cgacgggcga ataattttct ccaatctttt ttgtggaagg ggtgcacagg caatggcgaa
     4321 aaaggggcgc atcgaggttt tcgagcagtt ttgcaaaagc tgcggactgt gtgtggctgc
     4381 ctgtccggtg aaggtccttc gtatttccga aaagaccaac gccaaggggt atcgcccggt
     4441 cgaacagttc aaggaggggt gcatcgggtg cggcatgtgt gcgatgacct gtcccgatgc
     4501 ggcgatcgaa gttttcaaaa ttgaagagta gtttgtcttg ccggaggtga agtagacatg
     4561 ggacgagttt tgatgaaagg tactgaagct attgccgagg cggccattca ggccggctgt
     4621 aaatacttct tcgggtatcc gattacgccg cagaacgaga ttccggagta tatgtcggcg
     4681 catcttcccg aggtcggggg agtgtatgtg caggcggaga gcgaagtggc ctcgatcaac
     4741 atggtccttg gaggaggcgc cacagggtat cgggtgatga catcctcctc gagccccggt
     4801 atttcgctta tgtcggaagg gatttcctac gttgcaggtt gtgaaattcc cgcggtcatc
     4861 gtgaacgtga tgcgcgccgg tccgggattg ggcggcattc ttcctggaca ggccgactat
     4921 cttcagtcga ccaagggggg cggcaacgga gactataacc tgattgtcct cgctcccagc
     4981 acgttgcagg agtgcgtcga tcttacccag ctcgctttcg atcttgccca gaagtaccgc
     5041 aatcctgtca tggttgccgc cgacgggttc atggggcaga tgatggaagc tgttgagatt
     5101 aagccgagtc cgtcgaaaga ccagccggcc gacccgtcat gggctctcgg tttcatggga
     5161 gaaaggggcg gaaagcgcag ccacctgcat agcctcttcc tctctccgga acctttggag
     5221 gagcacaaca gaaaactgca gaagaagtac caggcaatga aagatgccga gcagcgctgc
     5281 gagtgctaca tgatggacga cgccgaggtg ctcatcgccg cctacggtac aacagcccgc
     5341 atctcccgct ccgccgtgaa caatctgcgc gctgaagggc tcaaggtcgg catgatccgc
     5401 ccgatcacac tgtggccgta tccttacgag gctttcaaga agcttccttc caccgtcaag
     5461 cacattcttg tttcggagat gaacgccgga cagatggtgg atgacgtgaa agttgccaca
     5521 ggttgcaagt accctgtctc cttctacggc cgcatgggcg gattcgcccc ctcggtcggg
     5581 gagatagaga acgaatgccg acggattctc ggcctgtaag ggaggggatt tctgatgagt
     5641 gaagtaaaag tctacgaacg tccgaagagt tggatgccga atgtcagcag ccactactgc
     5701 cccggttgcg ggcacggaat tgcccaccgt ctggtctgcg aggccatcga cgaactcggc
     5761 atacagaacg ataccatagg cgtcgcgccg gtagggtgcg cagcaatgat ct
//
LOCUS       DAOTIL010000052         5808 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminobacteriaceae bacterium isolate AMR_MDS_3026
            NODE_16350_length_5808_cov_3.638450, whole genome shotgun sequence.
ACCESSION   DAOTIL010000052 DAOTIL010000000
VERSION     DAOTIL010000052.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868050
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminobacteriaceae bacterium (wastewater metagenome)
  ORGANISM  Aminobacteriaceae bacterium
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae.
REFERENCE   1  (bases 1 to 5808)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5808)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:19:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,224
            CDSs (total)                      :: 3,198
            Genes (coding)                    :: 3,184
            CDSs (with protein)               :: 3,184
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5808
                     /organism="Aminobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16350_length_5808_cov_3.638450"
                     /isolate="AMR_MDS_3026"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:3034609"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1335)
                     /locus_tag="P5201_01880"
     CDS             complement(<1..1335)
                     /locus_tag="P5201_01880"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase"
                     /protein_id="HRV97315.1"
                     /translation="MMTIPSMDEFFGPEGLLNKRFSSFEYRKEQQDLAEEVHKALSAE
                     GEFILAAEAPPGVGKTFALLVPAMLRAAETGETVLVLTAGIPLQEQLIQKDLPLLNEL
                     LGLSLPFGLLKGKGNYACRSRAEEVFEGGEGRQGYLSHRDGGSSSRTVEEWLRTTTTG
                     DLSELSLPPNSPSVAGIAASSRSCRGFRCPRRGECFVQRVLSEAREWRVVVANYHLFF
                     SYLLGAGKPFPAPFDLLICDEAHHLPEAARSSMTVSVSDDDVVRLLRSRVFTDTLGRL
                     EKSGRGVQNAAALSAEIRQESARFFELLDVLLPGRKENITVRNEEMLRQQRILSGKML
                     ELYNVFVPLVSDDETPDVSEDGGLSSWMEECGRIRRSLAWCAEVEKYPSWAYWKNERS
                     LLSAPVSPGEELSSGFFTSSAEKMIFISATLALGGKTDFWERETGIHPDRLFI"
     gene            1446..1727
                     /gene="rpsT"
                     /locus_tag="P5201_01885"
     CDS             1446..1727
                     /gene="rpsT"
                     /locus_tag="P5201_01885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009166223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000312 - plastid small ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S20"
                     /protein_id="HRV97316.1"
                     /translation="MPNKKSAEKRMKTSERNRLYNRFWTTRCKNAVKKVLEAVEGQDE
                     ALAQTRLAIAQSVIDKAVVKGVMHGNTAARRKSMITNRVKALSGEKQPA"
     gene            complement(1812..2747)
                     /locus_tag="P5201_01890"
     CDS             complement(1812..2747)
                     /locus_tag="P5201_01890"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRV97317.1"
                     /translation="MPHLRVIASGGASARRLVAEELEALSKKGRAFAGTHEGGEWPAL
                     IAAGRSAGLFDDKRVSVVESAELLGPFPDELLPYLEGDDASEVLLLVFEKEPGKTFSP
                     AAKKKIVQIKGESVPFWSAQRKKWLLDLAGKEGVALSDDAAALLVELLEDPEEVRAEL
                     QKLGSYAHGAPVDAGMVKELSFDEGRNTMLKFLDAFCQARAPEVFAALERFKQEPSML
                     PLLTGLYNRIRPALYLGIFAPAGGERIRSALEIKEYALKMGREALRRYSSKALTELSF
                     GLLVLSWKEKTSFAEGWPGFESLLMTCMASAEKRQ"
     gene            complement(2747..5233)
                     /gene="leuS"
                     /locus_tag="P5201_01895"
     CDS             complement(2747..5233)
                     /gene="leuS"
                     /locus_tag="P5201_01895"
                     /EC_number="6.1.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008712795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004823 - leucine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006429 - leucyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="leucine--tRNA ligase"
                     /protein_id="HRV97318.1"
                     /translation="MGYDFQSIEKKWQHTWESEGTFNADADPARKKFYCLEMFPYPSG
                     ALHMGHLRNYSIGDMMTRFLWKRGFNVLHPIGFDAFGLPAENAAMKYKTPANKWTWEN
                     IEHMTEQLKHMGCSYDWRRRVETCNPDYYKWTQWIFLQFFKKGLAYRKNAPVNWCESC
                     NTVLANEQVINDGHCWRCNTPVVKRNLEQWFLKITDYAQELLDDLDNLPGWPDRVKTM
                     QRNWIGRSEGARLTFTEKSTGEIIETFTTRFDTIYGVTFLALAPEHPFVEKMCALSPK
                     GDEIRAFVQKCVTQSAIERTAVGGEKEGFFTGFSAVNPVTGESVPILIANYILMDYGT
                     GAIMGVPAHDQRDFEFARKYDIPVKVVIQPNDESLDGVTMEHAFEDDGITCNSGSFDG
                     LPTAEAIPAMIEWGVEQGFCSREVNFRLRDWLISRQRYWGAPIPVVYCDCCGIVPVPE
                     DELPVLLPAEVKIVEGGKSPLTEDKEWLTVPCPKCGKPGRREADTMDTFICSSWYFFR
                     YCSAREDGAVFNVDEANYWMAVDQYIGGIEHACLHLIYARFFTKFFADLGLCNAREPF
                     TNLLTQGMVIKDGAKMSKSLGNVVDPDEIINTYGADTARLFILFAAPPANDLDWSERG
                     VEGANRFLNRVWRLVEENIALLSSVRERPSLPMERLSEQRMRDLKRKIHTTIRDVTRD
                     IADEKQFNTAVARLMELLNALSSFRPETDDESALFREGVDVLLACLNPFCPHITEELW
                     SLGGHKETLSKLPWPEVEPSALEMDSVTVVVQFNGKVREKEDFPAGLSKEELQARVFA
                     LPSVQKRLEGVEVVRVIVVPDKLVNVVVKG"
     gene            complement(5481..>5808)
                     /locus_tag="P5201_01900"
     CDS             complement(5481..>5808)
                     /locus_tag="P5201_01900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013048088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="slipin family protein"
                     /protein_id="HRV97319.1"
                     /translation="SAVELKELELPESMKRAMAKQAEAERERRAKIINAEGEFQAAQK
                     LSEAAKQMELSPITLQLRYLQTLREVSGGDKSSTTIFPLPLDLVKPFMEMFFDKDKKK
                     AGGGDA"
BASE COUNT         1168 a         1782 c         1638 g         1220 t
ORIGIN      
        1 gataaaaaga cggtccgggt gaattcccgt ctcccgttcc caaaagtcgg tcttcccccc
       61 cagagcaagc gttgcggaga tgaagatcat cttctccgcg gaggatgtga aaaaaccgct
      121 cgaaagttcc tctcccggag acaccggggc gctgaggagg gagcgctcgt tcttccagta
      181 ggcccacgaa gggtatttct ccacctcggc gcaccacgcc aggcttctcc tgatacgccc
      241 gcactcctcc atccaggagg agagaccgcc gtcctcgctc acatcgggcg tttcatcgtc
      301 ggagacaagc ggaacaaaga cattgtaaag ctcaagcatc tttcccgaga ggatcctctg
      361 ctgccggagc atctcctcgt ttcgaacggt gatattctcc ttcctgccgg gcagaagaac
      421 gtccaaaagc tcgaagaaac gggctgactc ctgccgaatc tccgccgaca gcgccgctgc
      481 gttctgtacc ccccgtccgc tcttctccag tcttccgaga gtgtcggtga aaaccctgct
      541 ccggagcagc ctgaccacat cgtcgtccga cactgaaacg gtcatcgagg atcgggcggc
      601 ttccggaaga tgatgcgcct cgtcgcaaat caggaggtcg aacggcgccg ggaaaggctt
      661 tcccgcgccg aggaggtacg agaaaaagag atggtagttg gcgacgacaa cgcgccactc
      721 tcgcgcctca ctgagcaccc gctgcacaaa gcactctccc cgcctcggac agcggaatcc
      781 tctgcacgag cgcgacgacg cggcgatacc cgcgacggac ggagagttcg gaggaagcga
      841 gagctccgag agatcgcccg tcgtcgtcgt ccgcagccac tcctcgacgg tacgcgacga
      901 gctccctccg tccctgtgcg aaagataccc ctgcctgcct tctcctcctt cgaaaacctc
      961 ctccgcccgt gaccggcagg cgtaattccc cttccccttc agcagaccga aggggagcga
     1021 aagtccgagg agttcgttca gcaggggaag gtccttctgt atcagctgct cctggagagg
     1081 gatgcccgcc gtgaggacga gcaccgtctc ccccgtttcg gcggcgcgga gcatcgccgg
     1141 aacaaggagc gcgaatgtct tgccgacccc cggcggcgcc tccgccgcaa ggatgaactc
     1201 accttccgcg gaaagcgctt tgtggacttc ctctgcaaga tcctgctgct cttttctgta
     1261 ttcgaatgac gaaaatcttt tgtttaacag accttcaggg ccgaaaaact cgtccatcga
     1321 tgggattgtc atcatgtacc ctctttctga aactcgaaat tcccgggaaa tttctccgcc
     1381 tctcttgcta atataacaga aaccgtgcta gaatgtcgtg gttgtatcgg gaggaggtga
     1441 ggtacatgcc taacaagaaa tccgcagaaa aaagaatgaa aacgagcgaa aggaacaggc
     1501 tttacaaccg gttctggacg actcggtgca agaacgccgt gaagaaggtc ctcgaagctg
     1561 tggagggcca ggacgaggct ctcgcgcaga cgcgccttgc catcgcccag agcgtcatcg
     1621 acaaggctgt tgtgaagggc gttatgcacg gcaatacagc ggcacgaaga aaatccatga
     1681 tcacgaacag agtgaaggcg ctttccgggg agaagcagcc cgcgtagttc tttcgcacac
     1741 tcttcctttt cttccctttg aaattttgaa cactataaag ctgaacgcgc gacgccgctg
     1801 ttcggctttt tttattgtct cttctccgcg gatgccatac aggtcatgag taggctctcg
     1861 aaccccggcc acccctcggc aaaagaggtc ttttccttcc acgaaagcac gaggagaccg
     1921 aatgaaagct ccgtcagcgc cttcgacgaa tagcgccgca gcgcctccct ccccattttc
     1981 agcgcgtact cctttatctc cagggcgctc cggatcctct ctcctcccgc cggagcaaaa
     2041 atgccaaggt agagcgcggg gcgtattctg ttgtacagtc cggtgagcaa gggaagcata
     2101 ctgggctcct gcttgaagcg ctccagcgcg gcaaacactt cgggcgcccg cgcctggcag
     2161 aacgcatcca ggaatttgag catcgtgttc cgcccctcgt cgaaggagag ctccttgacc
     2221 attccggcat ctacaggggc cccgtgcgca taggaaccga gcttttgcag ttcggcgcgc
     2281 acctcctccg ggtcctccag gagttccaca agaagggccg ccgcgtcgtc cgacaaggcg
     2341 accccctctt ttcccgcgag atcaagcagc cacttcttgc gctgggccga ccaaaaggga
     2401 acggattccc ccttgatctg tacaatcttc ttttttgcag cgggggagaa ggtcttcccc
     2461 ggctccttct cgaaaaccag aaggagcacc tcggaggcgt cgtcaccttc aagatacggc
     2521 aggagctcat ccggaaacgg gccgagaagc tcggcggact ccaccacgga aacgcgtttg
     2581 tcgtcgaaaa ggcccgcgga acgcccggcg gcgatgagcg caggccactc gcctccctcg
     2641 tgcgttcccg cgaacgccct ccctttcttc gacagcgctt ccagttcttc cgcgacaagg
     2701 cggcgcgcgg aggcgccgcc cgacgcgatg actctcaggt gaggcactag cccttcacca
     2761 ccacgttgac cagtttatcg ggtaccacaa tgacgcggac tacttcgacg ccctcaagcc
     2821 gcttctgcac cgacggaaga gcaaaaacac gcgcctgcaa ctcctctttt gaaagccccg
     2881 ccgggaagtc ctctttttcg cgtaccttgc cgttgaactg caccacgacg gtaacggaat
     2941 ccatctccag cgccgaaggc tccacttcgg gccatggaag ctttgacagg gtttctttat
     3001 gcccgccgag cgaccacagc tcctcggtta tatgagggca gaaggggttg aggcaggcca
     3061 gcagaacatc gacgccctcc cggaagagcg cgctctcgtc gtccgtctcc ggacggaagg
     3121 atgagagcgc gttgagcagc tccataagcc gtgcgacagc cgtattgaac tgtttttcgt
     3181 cggcgatgtc ccgcgtgacg tcgcgtattg tcgtgtgaat tttccgcttc aggtcgcgca
     3241 tccgctgttc ggaaagacgc tccatcggaa gagaggggcg ttcccgcacc gaagagagca
     3301 gcgcgatatt ttcctccaca agacgccata ccctgttcag gaagcggttt gctccttcaa
     3361 cgccgcgctc cgaccagtcg aggtcgtttg caggaggcgc cgcgaagagg ataaagagcc
     3421 gcgccgtatc agcgccgtag gtattgatga tctcgtccgg gtcgacgacg ttgcccaggg
     3481 atttcgacat cttcgcgccg tccttgatga ccatcccctg cgtgaggaga tttgtgaacg
     3541 gctcgcgggc attgcacaaa ccgagatccg cgaagaattt ggtgaagaag cgggcgtaga
     3601 tcaggtggag gcatgcgtgc tcgatgccgc cgatatactg gtccacggcc atccagtagt
     3661 ttgcttcatc cacattgaag acggcgccgt cttcgcgcgc cgaacagtag cggaagaagt
     3721 accaggagga acagatgaac gtatccatgg tgtccgcttc gcgcctgccg ggcttgccgc
     3781 acttcgggca ggggacggta agccattcct tatcttcggt cagaggcgat ttcccgcctt
     3841 caacaatttt cacttctgct ggaaggagca cggggagctc gtcctccgga accggtacga
     3901 tgccgcagca gtcgcagtac accacgggaa tgggagcgcc ccagtagcgc tggcgggaga
     3961 taagccagtc tcgaagccgg aagttgacct cacgggagca gaagccctgc tcaacgcccc
     4021 actcgatcat ggcagggata gcctccgcag tgggaagacc gtcgaaggat cccgagttac
     4081 aggtgattcc gtcgtcctcg aaagcatgct ccatcgttac gccgtcgaga gattcatcgt
     4141 tcggctggat aacgaccttc accggaatat cgtacttgcg cgcgaactcg aagtccctct
     4201 ggtcgtgcgc aggaacgccc atgatggctc ctgtaccgta gtccatcaga atgtagttcg
     4261 cgatcagtat gggaaccgac tcgccggtga caggatttac cgcagagaat ccggtgaaaa
     4321 agccttcctt ctcgccgccg acggctgttc tctctatggc gctctgagtg acgcacttct
     4381 ggacgaacgc tctgatctcg tctcccttcg gggagagcgc gcacatcttt tcgacaaacg
     4441 gatgctccgg cgccagtgcg aggaaggtca ctccgtagat agtgtcgaag cgggtggtga
     4501 aagtttctat gatttcacct gtactcttct cggtgaaggt gaggcgggcg ccttcggaac
     4561 ggccgatcca gtttcgctgc attgtcttca ctcggtcagg ccaccccggc aggttgtcca
     4621 gatcgtcaag cagctcctgc gcatagtcgg taattttgag gaaccactgc tccaggttgc
     4681 gcttgacgac aggggtgttg catcgccagc agtgcccgtc gttgatgacc tgttcgttgg
     4741 cgagcaccgt gttgcagctc tcgcaccagt tcaccggcgc gttctttctg taggcgagcc
     4801 ccttcttgaa gaactggagg aagatccact gggtccactt gtagtagtcg gggttgcagg
     4861 tctcgaccct tcgccgccag tcgtagctgc accccatgtg tttgagctgt tccgtcatgt
     4921 gttcgatgtt ctcccaggtc cacttgttgg cgggagtctt gtacttcatc gcggcgttct
     4981 cggccgggag accgaacgcg tcgaatccga tcggatgcag gacgttgaac ccccgtttcc
     5041 acaggaaacg ggtcatcatg tcgcctatgg agtagttccg caggtgcccc atgtgcaacg
     5101 cgccgctggg gtacggaaac atctccaggc agtagaattt cttccgtgcc ggatcggcgt
     5161 ccgcattgaa cgtcccttcg ctctcccacg tatgctgcca ttttttctcg atggattgaa
     5221 aatcgtagcc cataaccctt cctccttgat cacagcgccc gttcgcagga cagacgcaaa
     5281 cgaccgtccc ggaagaaagt cttctctcgt gaaggctctc tccttgaaag gccgtgtgcg
     5341 tccgtttcaa aacagtcgtc cgaaatggtc ttgtacaggt attatatacc ggaatcgcct
     5401 gattccacta tccgttttgt ttacgggcgg ggcaggccgc gtgcggcagc ctgccgcctc
     5461 ccattgccga ctattgacgc ttacgcgtcg ccgccgcccg cttttttctt atccttgtcg
     5521 aagaacatct ccataaaggg cttcaccagg tcgagcggca gcgggaagat ggtcgtggag
     5581 gatttgtctc cgccgctgac ttcgcgcagc gtttgcaggt agcggagctg gagcgtgatg
     5641 ggtgaaagct ccatctgttt tgccgcctcg ctcagttttt gcgcggcctg gaactcgccc
     5701 tcggcgttga tgatcttggc gcggcgttcg cgctccgcct cggcctgctt cgccatggcg
     5761 cgcttcatgc tctcgggcag ctccagttcc ttcagttcga cggcgctg
//
LOCUS       DAOTIL010000053         5641 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminobacteriaceae bacterium isolate AMR_MDS_3026
            NODE_16995_length_5641_cov_3.542607, whole genome shotgun sequence.
ACCESSION   DAOTIL010000053 DAOTIL010000000
VERSION     DAOTIL010000053.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868050
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminobacteriaceae bacterium (wastewater metagenome)
  ORGANISM  Aminobacteriaceae bacterium
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae.
REFERENCE   1  (bases 1 to 5641)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5641)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:19:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,224
            CDSs (total)                      :: 3,198
            Genes (coding)                    :: 3,184
            CDSs (with protein)               :: 3,184
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5641
                     /organism="Aminobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16995_length_5641_cov_3.542607"
                     /isolate="AMR_MDS_3026"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:3034609"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..504
                     /locus_tag="P5201_01905"
     CDS             <1..504
                     /locus_tag="P5201_01905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006301115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-N-acetylmuramoyl-pentapeptide-
                     transferase"
                     /protein_id="HRV97320.1"
                     /translation="IVSIGMLNAVNVTDGLDGLAAGAALFSFIGAIVLLPLESFLLYP
                     ALAGSALCAGFLWHNAHPAKVFMGDIGSHFLAGMLVALCAHSEYFIAVIPLGFLFGVE
                     IVSVAMQLIAIHCFKRKVFRMSPLHHHFELLGWSETQIVTRFWIIHGAGLAFLALFVQ
                     GILWKAV"
     gene            506..1897
                     /gene="murD"
                     /locus_tag="P5201_01910"
     CDS             506..1897
                     /gene="murD"
                     /locus_tag="P5201_01910"
                     /EC_number="6.3.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015556637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008764 -
                     UDP-N-acetylmuramoylalanine-D-glutamate ligase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanine--D-glutamate
                     ligase"
                     /protein_id="HRV97321.1"
                     /translation="MMPLDVDSFSGKRVSVIGAGISGAGLARLAHRLGAQVFVSDEKK
                     LQDQSLVALFQSHGIEWEDGGHTPRLFEADYILVGSGISPNAKPVTESNRRNIPVIGE
                     LDFISPFLRSRIIGVTGSNGKSTTTALAGYLLQKAGFSAEVAGNIGKSLADFAFVQRD
                     FLVVELSSFQLHWNTRFACDLAMVTNLAPDHIDWHGSYEEYIRAKAKILSTQSPGGFA
                     IVQETERSVLFGDREDDAHEVLAFSWNGMQSSHGRKLLADEKACHVLFRDETGAEELL
                     FSFDDVPLIGKHNMENAAFAAGAIRMLTGKGIMRGPLFSGFKGLAHRCEFVANIRGVT
                     YVDDSKGTNVAASATALRSITGPKIVILGGQGKGEEYAPLAEAVKSEAVAAVVLGAEK
                     ERIVKALNEAAFPHIHEVSSMEEAVQTASTLAFPQSTVLLSPACTSWDMYPNYKKRGE
                     HFQQLVLALEDER"
     gene            1984..3036
                     /locus_tag="P5201_01915"
     CDS             1984..3036
                     /locus_tag="P5201_01915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008711564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsW/RodA/SpoVE family cell cycle protein"
                     /protein_id="HRV97322.1"
                     /translation="MPLVLNAFGILMIFSLTSHTALEEFGSPFALGIRQVQWLSVSLL
                     FMVLVYLIPLSVWKEFSGVLWVFTLCCLVLTLIPGIGIKAGGARRWIGFGNLRFQPIE
                     LLSLAVAVHLSKLMTKSSKQGLHAFFSLTAPILGFSALPLIFQPNMGGTILIFALTMA
                     IHVETRGWSWPILFGGTGFAFFFFIIIQAGYRLRRYLAFLDPWEEPLDSGFQVIQGMV
                     AFANGGLLGVGVGKGLQKLNYLPAAHTDYIFATIGEEFGFVGSFMVVLLFCIWTFRAY
                     STYRKAEDPFHASLIWGLSASMLIPFFINLGGVLKLMPLTGIPLPFISYGGSSLLFMW
                     IRVGLIMRVCKEASLS"
     gene            3033..4118
                     /locus_tag="P5201_01920"
     CDS             3033..4118
                     /locus_tag="P5201_01920"
                     /inference="COORDINATES: protein motif:HMM:NF016027.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine--N-acetylmuramyl-
                     (pentapeptide) pyrophosphoryl-undecaprenol
                     N-acetylglucosamine transferase"
                     /protein_id="HRV97323.1"
                     /translation="MKRVCLVAGGTGGHIFPAIAFGKWITKHQEEVKVSFVCGSRPLE
                     QEIYEASHITPYYLPTSGSPLGIRSLSGKMRRLKEFGLSFISFKKLLRNCHVDACVLF
                     GGYVSFIPLMVCHLTGVPVFVHEQNSVAGKVTRLSSRLGKKVASGWEVCTPLSPDAFH
                     WTGVPVRGFSLREPQEAWELMALGKKLPEKKIIGVLGGSLMSQRLIQLMGPMLRAQEM
                     RNFFFLFLGDSQLADIAREFHGSPENISVIGKQWDMSNFYSVVDAVVTRGGASTLSEL
                     ATLGIPAVVVPWQEAADNHQERNALCFIEHNNGAIWHENDSPKAFQECILKILRNTQR
                     PPRSISKGDESESLWRLISSSIGREIL"
     gene            4119..5540
                     /gene="murC"
                     /locus_tag="P5201_01925"
     CDS             4119..5540
                     /gene="murC"
                     /locus_tag="P5201_01925"
                     /EC_number="6.3.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013048219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008763 - UDP-N-acetylmuramate-L-alanine
                     ligase activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate--L-alanine ligase"
                     /protein_id="HRV97324.1"
                     /translation="MDTTISNFDGIHRIHLMGIGGAGMSGLALLLAELGFEVSGCDMI
                     HTSYIDKVLKEEVAVVLGHGHAHMEKFLPDLLIYSSAIPEDNEEILAARARGIAVAKR
                     GEVLSWIFDSRCGIGVAGTHGKTTTSSMISLILEQAGFHPTLAIGGELCDIGVNAKLG
                     EGRFMVAELDESDGSFEKFHPEISVITNVDWDHVNFYPTFESVLEAFSRFFSNLKSDG
                     LLVFCGEDAGLARLLSLGRIPEGAISYGWGASWNWGASNVYHKPGGGVSFTLLHEGQR
                     VGEVELAISGEHNILNTLASCAVAHRLGIPFPVIKRTLRDFKGAKRRLQHVGSFEGID
                     VYDDYGHHPREIAATLGTLEHMFPERPLRVVFQPHRYTRTQALFKDFASVLAVADTLI
                     LLPIYQADEEPISGITSLLISDHLPAEKKQACLMPKTLDDAVFLLEDLCEEGDIVLTI
                     GAGDVSLIGDNLLAKAKTHLVRV"
BASE COUNT         1327 a         1291 c         1452 g         1571 t
ORIGIN      
        1 atagtatcaa tagggatgtt aaatgcggtg aatgttaccg acggcctgga cgggcttgcc
       61 gccggtgcgg cgctgttttc attcattgga gcgatcgttt tgctgcccct tgagagtttt
      121 ctgctatatc ctgctctggc gggttctgct ctctgtgccg ggttcctttg gcataatgcg
      181 cacccggcta aggtgttcat gggagatata ggctcccact ttttggcggg tatgctcgtt
      241 gccctctgtg cgcacagtga atacttcatt gctgttatcc ctctcggttt tctctttggc
      301 gtggagatag tctcagttgc aatgcaactg atcgccattc attgtttcaa gagaaaggtc
      361 tttcggatga gcccgcttca tcatcatttc gaactccttg gatggagcga aacgcagata
      421 gtcactcgtt tttggataat ccatggagca ggacttgcgt tccttgcact ttttgtacag
      481 ggcattcttt ggaaggcggt gtgagatgat gccgttggac gtggacagtt tctcaggaaa
      541 aagagtttcc gtcatcgggg cgggaatcag cggagcaggt ttggctcgtt tggctcatcg
      601 tcttggagcg caagtttttg tctcggatga aaaaaaactg caggaccaat ccctcgtcgc
      661 tctttttcag tctcatggga ttgagtggga ggatggagga cacacccccc gtctttttga
      721 agcagattat attttggtag gttcaggtat ttctcccaac gcaaaacctg tgacggagag
      781 taatcgaagg aatatcccgg tgatagggga gttggatttt atatcgccgt ttctccgaag
      841 cagaattatt ggtgtaaccg gaagcaacgg aaaaagtaca acgaccgcgc ttgccggcta
      901 tcttttacag aaagcgggct tctccgcaga agttgcgggc aacataggaa aatcgctagc
      961 cgactttgcc tttgtgcaac gcgactttct cgtggtggag ttgagcagtt ttcaacttca
     1021 ctggaacacc agatttgcat gcgatcttgc catggtgaca aacctggctc ctgatcatat
     1081 cgactggcac ggcagttatg aagagtacat tcgggcaaag gcaaagatcc tctcaacaca
     1141 atccccggga ggcttcgcta ttgttcagga aacggaacgc agcgtccttt tcggagatcg
     1201 cgaggatgac gctcacgaag ttctagcttt ctcgtggaat ggaatgcaat catctcatgg
     1261 aagaaaactt cttgctgacg aaaaagcatg tcatgtacta ttccgggacg aaacaggagc
     1321 cgaggaactc ctcttctctt ttgacgacgt tccccttata ggaaaacata acatggaaaa
     1381 tgcagcattt gcagccggag ctatacgtat gctcaccgga aaaggtatta tgcgcggtcc
     1441 gcttttttca ggttttaaag ggttggcaca taggtgcgag ttcgtggcga acattcgcgg
     1501 cgtaacctat gttgatgatt cgaaaggaac aaacgtagcg gcgagcgcta ccgctctcag
     1561 atcaataaca ggacctaaaa ttgttatact gggcggtcag ggaaagggag aagaatatgc
     1621 ccctttggca gaagcggtaa aaagtgaagc agtagccgct gtcgtcctcg gagctgagaa
     1681 agaacggata gtgaaggctt taaatgaagc ggcatttcct catatccacg aagtctcctc
     1741 gatggaggag gctgttcaaa cagcatctac actggctttt cctcaaagca cagtgcttct
     1801 ttctccggca tgtaccagct gggacatgta ccctaactac aaaaaacgag gagagcactt
     1861 tcagcagctc gtgcttgctc ttgaagacga gaggtaaaga tgagagtaaa cgccgaacag
     1921 acgatcgaat tccgttacga aaaaccgaag cttcattcag ggggagatcc ctttttatgg
     1981 atcattccgc ttgtgctcaa tgctttcggc atccttatga tcttttcgtt gacgtcgcat
     2041 accgcccttg aggagttcgg ctctccattt gctcttggca tacggcaagt tcaatggcta
     2101 tcggtctctt tgctgttcat ggtactcgtc tacctgatac ccctctcagt atggaaggag
     2161 ttcagcggcg ttctctgggt ttttacactg tgctgccttg tactgacctt gatacccggt
     2221 atcggaatca aagccggtgg agccaggaga tggataggct tcggcaacct tcgtttccag
     2281 ccgatcgaac tgctttctct tgctgtcgcc gttcatctct ccaaacttat gacaaagtct
     2341 tcaaaacagg gcctgcacgc cttcttttca ctgacggcgc ccattctcgg tttttccgcg
     2401 ctccctctta tcttccagcc gaacatggga ggaaccattc ttatttttgc cctgacaatg
     2461 gctatccacg ttgagacaag agggtggagc tggcctattc tttttggagg cacaggattt
     2521 gccttcttct tttttatcat tattcaagcc ggttaccggc ttcgacgata tttggcgttt
     2581 cttgatccgt gggaagagcc cctcgacagc ggattccaag ttattcaagg catggtggct
     2641 ttcgctaatg gaggtcttct tggcgttggg gtagggaagg ggctccagaa actgaactat
     2701 cttccggcag cacatacgga ttatatattt gcgacgatag gagaggaatt cggtttcgtc
     2761 ggttctttca tggttgttct gctgttctgt atctggacat ttcgcgcgta ttccacgtac
     2821 cgaaaagcgg aagatccatt ccatgcatcg cttatctggg gactttcagc ctcaatgctc
     2881 attccttttt tcataaacct cggtggagtt ttaaagctga tgccgctcac cggcatacca
     2941 cttcctttca ttagctacgg cggaagttcg cttcttttta tgtggatacg tgttggattg
     3001 atcatgagag tatgcaagga ggcgagtctc tcatgaaacg ggtctgtctt gtcgcgggag
     3061 gcaccggcgg tcacattttc ccggcgatag ctttcggcaa atggataaca aaacaccaag
     3121 aggaggtgaa ggtctctttt gtttgcggtt ctcgtcctct agaacaggaa atctacgagg
     3181 catcccacat tactccgtat tatcttccga caagcggctc tcctttggga atacgctctc
     3241 tttccggtaa gatgcgccgg ttgaaggaat ttggtttgtc ttttatatca tttaaaaaat
     3301 tattaaggaa ctgccatgtg gatgcatgcg ttctgttcgg agggtacgtt tcgtttattc
     3361 cattaatggt ttgtcatctt acaggcgttc cagtttttgt tcatgaacaa aacagcgttg
     3421 caggcaaagt gacacggctt tcttcacgtc ttgggaaaaa ggtggcttca ggatgggaag
     3481 tctgcactcc tctttctccg gacgctttcc actggaccgg cgttccggtt cgcggcttct
     3541 ctttgagaga accgcaagaa gcttgggaat taatggcttt agggaaaaaa cttccagaga
     3601 aaaaaataat cggagttctc ggaggttcac ttatgagcca acgactgata caattaatgg
     3661 gtcccatgct tcgggcgcag gaaatgagga attttttctt tttgttcctc ggcgattctc
     3721 agcttgccga catagcaagg gagtttcatg gcagcccgga aaatatatct gttatcggta
     3781 aacaatggga tatgagcaat ttctactctg ttgtcgacgc cgtagtgact cgcgggggtg
     3841 cttctacgct ttctgaatta gcaaccctcg gtattcctgc ggttgttgtt ccatggcaag
     3901 aagctgccga taatcaccag gagagaaacg ctctctgttt tattgaacac aataacggcg
     3961 ctatctggca tgaaaatgat tccccgaaag cgtttcaaga gtgcatactg aaaattcttc
     4021 gaaatacaca gagaccacca cgaagtattt cgaagggaga tgagtctgaa tctttatggc
     4081 ggctgatctc gtccagtatt ggaagggaga ttttataagt ggatacgact atatcgaatt
     4141 ttgatggcat tcacagaatt catctcatgg gcatcggcgg cgcaggtatg agcggattgg
     4201 cccttttgct ggctgaattg ggttttgaag tgagcggctg cgacatgatc catacttctt
     4261 atatcgacaa ggtactcaag gaagaagtgg cagtggttct gggacacgga catgctcata
     4321 tggaaaagtt tcttccggat cttttaatat acagcagcgc catcccggaa gacaatgaag
     4381 aaatcctcgc tgccagagca cgcgggatcg ctgtagcaaa aaggggagaa gtactgagct
     4441 ggatttttga ttcgcgttgc ggaatagggg ttgccggaac tcatgggaaa accacaacgt
     4501 cttcaatgat ctcgcttatt ttggaacagg ccggttttca tcccaccctg gctatcgggg
     4561 gagagttgtg tgatatcggc gtcaacgcaa aacttggaga aggacgcttt atggttgccg
     4621 aactcgatga aagcgacggt tcttttgaga aatttcatcc cgaaatttcc gtcattacaa
     4681 acgttgactg ggatcacgtc aatttttacc ctacgtttga aagtgttctt gaagcttttt
     4741 cacgtttttt ctccaatctc aaatcagacg gtctccttgt tttctgcggt gaggatgcgg
     4801 gccttgcgcg cttgctgtcc cttggccgta ttcctgaggg agccatatcg tacggctggg
     4861 gcgcttcttg gaattggggg gcaagcaatg tctatcacaa accgggcggc ggcgtcagtt
     4921 tcacgcttct gcacgaggga caacgagtag gcgaggttga gctggcaata tctggagaac
     4981 ataacatatt aaacacgctc gcttcctgtg cggttgcaca tcgtctcgga atcccgtttc
     5041 ctgtcattaa aagaaccctg cgggacttta aaggggcgaa acgccgtctc cagcatgtcg
     5101 gaagttttga gggtatcgac gtctatgacg actatggcca tcacccgcga gagatcgcag
     5161 ccaccttggg aacgctggaa catatgttcc ctgaaaggcc gcttcgggtt gtcttccagc
     5221 cgcacagata cacgaggacc caggctttgt ttaaagactt cgcatctgtt cttgcagtcg
     5281 cagacacact gattcttctc ccgatctacc aggctgacga agaaccgatc tctgggataa
     5341 cgtcgctcct aatttccgac catctaccgg ccgagaaaaa gcaagcgtgc ctgatgccaa
     5401 aaactcttga tgatgctgtt ttccttttgg aagacctttg cgaggaagga gacatcgtct
     5461 tgaccattgg tgcaggagac gtgtcgttaa taggagacaa tttgcttgca aaggcaaaga
     5521 cacatttagt gcgtgtgtaa ttttccaaac cagcgttcag gggtttgagg aaaagtgcat
     5581 tcttttctta ttgctttcct gctttttact cgagccatct ttgtgacgca taatgggtaa
     5641 a
//
LOCUS       DAOTIL010000054         5632 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminobacteriaceae bacterium isolate AMR_MDS_3026
            NODE_17038_length_5632_cov_4.207459, whole genome shotgun sequence.
ACCESSION   DAOTIL010000054 DAOTIL010000000
VERSION     DAOTIL010000054.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868050
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminobacteriaceae bacterium (wastewater metagenome)
  ORGANISM  Aminobacteriaceae bacterium
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae.
REFERENCE   1  (bases 1 to 5632)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5632)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:19:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,224
            CDSs (total)                      :: 3,198
            Genes (coding)                    :: 3,184
            CDSs (with protein)               :: 3,184
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5632
                     /organism="Aminobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17038_length_5632_cov_4.207459"
                     /isolate="AMR_MDS_3026"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:3034609"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..786
                     /locus_tag="P5201_01930"
     CDS             <1..786
                     /locus_tag="P5201_01930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013049170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004180 - carboxypeptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HRV97325.1"
                     /translation="PAEEAGMHSGAPKMIEEGALDGVEAIGGLHVWALLESGKLGYRI
                     GPVMASADIWDLKIQGKGGHGAMPHHAVDPTVTAATVISTLQTVVSREIDPQETVVLS
                     IGKLEAGTAVNIIPDTARVAGNVRTTNPEIRARMQGIMKRVADGICAAMRCTAELVYT
                     PIYPVTVNDPAMTQLMRSVAVEVFGEERVVELPVAMGSEDFSYYGEKIPAAYVFLGIA
                     DEAKRTGNQHHNPKFNVDDEPLPKGAALLAGFAARFTAGESAS"
     gene            833..2380
                     /locus_tag="P5201_01935"
     CDS             833..2380
                     /locus_tag="P5201_01935"
                     /inference="COORDINATES: protein motif:HMM:NF012245.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HRV97326.1"
                     /translation="MYYWNGKASKGEISREDAQKMAGDVIFMLRYEGDNYIFGYDMEN
                     KVSIPFQSHERGKFLDVKDQDGNWVQRDLREIAKTKGKGFYTYNWLNSNTKRVEPKVA
                     YVRYFEPFDWWYGTGVYVEDIKAKALRSTMVQAGILGVSILLICISIALLTRRFITAP
                     LRRVVLLSERAGSGDLTVNRNDFAYSGKDEIGLMADALSSMISNQAKTVRGIVGTVSE
                     VSSAAENLSALSEETEASLEEMQKFLAQVGEMTESGAAAAEEANAGVAEVAEGAQSVS
                     RSATTGAEAGSQADSIVKGTVDQFESVIANVARVEKQGEANMQAVSSLASSVQEITGF
                     VVTISRIADQTNLLALNAAIEAARAGEAGRGFAVVADEVRKLAEESNEAAKAIRVLIN
                     GLEEKAGASVQSIESSDKILKETSALSQRSMEELRKGMGAVQRVISVISDLAAVSEEQ
                     SASSSEMTRAVDQIARTTSSIAGTVDTIRASSAETVQASMQIAEQATKLADRASVLQS
                     MIAEFRV"
     gene            2655..3500
                     /locus_tag="P5201_01940"
     CDS             2655..3500
                     /locus_tag="P5201_01940"
                     /inference="COORDINATES: protein motif:HMM:NF013539.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HRV97327.1"
                     /translation="MLRERIASRLHEMTPGQKLVANYILEYAREAAFLTASQLGERVG
                     VSETTVIRLAHLLGFSGYQQLRSAMTNLLMDRLSTLERLKDYASTQEDDFFERALRKD
                     LETLTLTLSTICADDLKKLGAAIASAECVYLAGHRSSFSLAYYLSFYLSWILPNVKIV
                     SPDMPFEMLANAPKKSIVLGISFPRYSTWTVQVLETASELGLATACLTNDLASPLAVL
                     SEYVLAVPYKPISFIDSFAAPMSVLNCLILSVAQQLGDDVTKKLGILEKRWKEDDVYT
                     QQRGR"
     gene            3581..4927
                     /locus_tag="P5201_01945"
     CDS             3581..4927
                     /locus_tag="P5201_01945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005659548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate aminotransferase family protein"
                     /protein_id="HRV97328.1"
                     /translation="MDLGHLIPRSFKGNLRTAVRGEGVYIYDEDGNAYMDGCCGALLS
                     SIGHGVKEIADAIHQQLLTMEFAHPSRWNNKVSVEAAEEITSLTQEGLDYVWLVSGGS
                     EAIESAMKIARQYFVERDGDGSGKYVMIARWNSYHGSTVGTMGLAGSMGRRRTFAPLF
                     MEGPKIQAHYCYRCPHNLKYPECNVACARALEGEILRIGPASVAAFFAEPIVGSTVGA
                     LHPPKEYWPIVREICSKYDVLLVADEIMTGMGRSGKPFCTQHWNVTPDIICSAKALAG
                     GYSPVGAMIVKKEIAETIRDGSGAFQHGHTYNGNPVTAAAVTATIRYMKKNNVFENAA
                     AMGARFDEKIPALNEIPIVGEVRGIGMMRGIEIVADRKTKAPFSPSVKAAALVTEECM
                     KRGLVVYPGTGQIGGVAGDQFLFAPPLVATKEEMDMMTDRLEAGLKAAAEALKGKG"
     gene            4970..>5632
                     /locus_tag="P5201_01950"
     CDS             4970..>5632
                     /locus_tag="P5201_01950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005659550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TAXI family TRAP transporter solute-binding
                     subunit"
                     /protein_id="HRV97329.1"
                     /translation="MKKGILALALIGIMVFASTGMADTFITIGSGGVGGTYYPLGGAM
                     AEVLTNAGIGVRATSRSTSASRENCRLVASGRAHIGMTMGSALYQAITGTEAFEPDGK
                     LPLQILMSMYPAPQHLVTVKGSGITKLEDIKGKRVSVGAPGGGDQILTNLILAAAGID
                     PEKDFTKQQLTQPEGVMALKDGNVDAVFWNFAAPGSAVLEVAAQRDVVMIPLEEALVQ
                     KII"
BASE COUNT         1194 a         1527 c         1801 g         1110 t
ORIGIN      
        1 cccgccgagg aggccggaat gcactcgggc gcaccgaaga tgatcgagga aggggcgctc
       61 gacggtgtag aggccatcgg cggtctgcac gtgtgggcgc ttctggagtc gggcaagctg
      121 gggtatcgca tcggtcctgt catggcttct gcggacatct gggatctgaa gattcagggg
      181 aagggcgggc acggcgcgat gccgcatcac gccgtcgatc cgacagtaac cgccgcgacc
      241 gttatctcga cgctgcagac agtcgtgagc agagagatcg atccgcagga gaccgttgtg
      301 ctcagcatag ggaaactgga agccggaact gccgtgaaca tcatcccgga cactgcgcgg
      361 gtggcgggaa acgtccgtac gacgaacccg gagatacgcg cccggatgca gggcattatg
      421 aagcgcgtag ccgacggaat ctgcgccgcg atgcgctgca cggccgaact tgtctatacc
      481 ccgatctacc cggtgacggt gaacgatccc gctatgacgc agctcatgcg ctcggtcgcc
      541 gtcgaggtgt tcggcgagga gcgcgttgtg gagcttcccg tcgcgatggg ttccgaagac
      601 ttcagttact acggggagaa gattcccgcc gcgtacgtgt tcctcgggat cgccgacgag
      661 gcgaagagga ccggcaatca gcaccataac ccgaagttca atgtcgatga cgagcccttg
      721 ccgaaggggg ccgctctgct tgccggtttc gcggcaagat ttacggcagg tgaatccgct
      781 tcctgaccta tcccggccgg gtgctcattc ttctttagaa tagcccttcg ctctgtacta
      841 ctggaacggg aaggcctcca aaggagagat ttcccgcgaa gatgcacaga agatggccgg
      901 cgatgtgatt ttcatgctca ggtatgaagg cgacaactat atttttggct acgacatgga
      961 gaacaaagtg tccataccgt tccagagtca tgaacggggc aagttccttg atgtgaagga
     1021 ccaggacggg aactgggtgc agcgcgacct gagagagatc gccaagacaa aagggaaagg
     1081 attctatacc tacaactggc tgaattcgaa cacaaagagg gtcgagccca aggtggcgta
     1141 tgttcgctat ttcgagccat tcgactggtg gtacggcacc ggcgtgtacg tggaggacat
     1201 caaggcgaaa gccttgcgca gtacgatggt gcaggccgga atcctcggcg tgtccattct
     1261 tttgatctgc atttcaatag cgcttcttac gcggcgcttc attaccgctc ccctgcgaag
     1321 agtggtgctt ctctccgaac gggcggggag cggcgacctc actgtgaaca ggaacgactt
     1381 cgcgtattcg gggaaggacg agatagggtt gatggcggac gcgttgtcgt ccatgatatc
     1441 aaatcaggcg aagacagtca gaggaatagt cggcacggtg tcggaagttt cgtccgccgc
     1501 ggagaatctc tcggcgctga gcgaagagac cgaggcgtct cttgaggaga tgcagaagtt
     1561 tcttgcgcag gtcggagaaa tgacggagtc gggggctgcc gcggcggaag aggcaaacgc
     1621 gggcgttgcg gaagtcgcgg agggggcgca gtctgtttcc aggtcggcga caaccggagc
     1681 ggaggcgggg agtcaggctg attccattgt gaagggaacc gttgatcagt ttgaatccgt
     1741 tatcgcaaat gtggccaggg tggaaaagca gggcgaggcg aatatgcagg ccgtttcgtc
     1801 gcttgcctcg tcggttcagg agatcacggg ttttgttgtg acgatttcgc gtattgccga
     1861 ccagacgaat ctgctcgccc tgaacgcggc tattgaagcc gcgagggccg gtgaggccgg
     1921 aagagggttt gccgtagtgg ccgatgaagt ccggaagctt gcagaggagt ccaacgaagc
     1981 tgcgaaggcg atacgcgtgc tcatcaacgg tctggaggag aaggcgggcg catccgtcca
     2041 gtcgatcgag tcctccgaca agatcctcaa ggagacgtcg gcgctctctc aacgatccat
     2101 ggaggagctt cgaaaaggaa tgggcgccgt gcagcgcgtg ataagcgtca tatccgacct
     2161 tgcagcggtt tccgaggagc agtccgcctc gtcgtccgaa atgacgcgcg ctgtggacca
     2221 gatagcgcgg acgacgtcct cgatagccgg gacggtcgac acgatacgcg cctcttccgc
     2281 ggagacggtg caggcgtcga tgcaaatcgc ggaacaggcg acgaaactgg ccgaccgggc
     2341 ttccgtgctg caaagtatga ttgcggagtt cagagtgtga gactgtagcg aggcttcctg
     2401 cggcggagtt tttccccccg gatgctcctg tgaagcattt atgtggcggg tagagctgtt
     2461 cgacaagagc cccgaaaaag cgtccttctc ttgatgaagg gcgctttttc gggcttaaaa
     2521 acctacatcg tttttctgtt cgcacaccct ggggaggtac ttaatttgac aaaatgcagt
     2581 ttttcgatta ttatccgtga aggaaatact tcattgtgca ttgattaagg attttttccc
     2641 ttcaaggagg gcgcatgctc agagaacgta tcgcatcacg actgcatgag atgacgccgg
     2701 ggcagaagct tgtggcgaac tatattctcg aatacgctcg cgaggcggct ttcctgaccg
     2761 cttcgcagct tggggagcgt gtcggggtaa gcgagacgac agtgatccgc ctggcgcatc
     2821 ttctgggatt ctccggctac cagcagttgc ggagcgcgat gaccaatctt ctgatggacc
     2881 gcctgtcgac gctcgaacgc cttaaggatt atgcgtccac acaggaggac gatttcttcg
     2941 agcgagcgct ccgcaaagac cttgagacgc tgacgctcac cctttcaacc atttgcgccg
     3001 acgatctgaa aaaactcggc gcagcgatag cgtccgcaga atgtgtgtac cttgccggac
     3061 atcgcagctc tttctctctt gcctactacc tttcttttta tctctcatgg attctgccga
     3121 acgtgaaaat tgtcagcccc gacatgccct tcgagatgct ggcgaatgcg ccgaaaaaaa
     3181 gtattgtcct cgggatcagc tttccacggt attccacgtg gacggtgcag gttcttgaaa
     3241 cggcctccga attggggctt gcgaccgcat gtctgacgaa cgacctggca agtcctctcg
     3301 cggttctgtc ggagtatgtc ctggccgttc cgtataaacc gatttcattc atagactcct
     3361 tcgcggcgcc gatgagcgtc ctgaactgcc ttatattaag cgtcgcccag cagttgggcg
     3421 acgacgtgac gaagaagctc gggatacttg aaaagcgctg gaaggaggac gatgtgtata
     3481 cgcagcagag gggccgctga aacgtgttgt acagcttcgg ttgtctgcgg cgcaggacgc
     3541 accacaaaag ttcgggaaaa atgaaaggag ttgcttctta gtggatcttg gacatttgat
     3601 accgcgaagc ttcaagggaa acctcagaac ggccgtccgg ggagagggcg tctacatcta
     3661 cgacgaggat ggaaacgcct acatggacgg gtgctgcggc gcattgctct caagcatagg
     3721 gcacggcgtg aaggagatag ccgacgccat ccatcagcag cttcttacga tggagttcgc
     3781 ccacccgtcg cgctggaaca acaaggtctc cgtcgaggcg gcggaggaga ttacctccct
     3841 gacgcaggaa ggtcttgact atgtctggct cgtcagcggc ggcagcgagg ccatcgagtc
     3901 ggcgatgaag attgcgcggc agtatttcgt cgaacgcgac ggcgacggtt ccggcaagta
     3961 cgtgatgatc gcccgctgga actcctacca cggaagtacc gtcgggacga tggggctcgc
     4021 cggcagcatg ggccgccggc gcacgttcgc accgctcttc atggaggggc cgaagattca
     4081 ggcgcactac tgctaccgct gcccgcacaa cctgaagtac cctgagtgca acgtggcctg
     4141 cgcccgcgct ctcgaaggcg agatcctgcg catcggcccg gcctcggtgg cggccttctt
     4201 tgccgagccc atcgtggggt ccaccgtcgg cgctctgcat ccgccgaagg agtactggcc
     4261 catcgtccgc gagatctgct ccaagtacga cgtcctgctt gtcgccgacg agatcatgac
     4321 cggcatggga cgatcgggca agcctttctg tacgcagcac tggaacgtga cgccggatat
     4381 catctgttcc gccaaggcgc tcgccggtgg atactcgccg gtgggggcga tgatcgtcaa
     4441 gaaagagatc gccgaaacga tccgcgatgg aagcggcgcc ttccagcacg gccacaccta
     4501 caacggaaat ccggtgaccg ccgccgctgt gacggcgacc atccgctaca tgaaaaagaa
     4561 caacgtcttc gagaacgccg ccgcgatggg cgcgcggttt gatgagaaaa tccccgcgct
     4621 gaacgagatt cccatcgtcg gcgaagtgcg gggcatcggg atgatgcgcg gtattgagat
     4681 cgtcgcggac cggaagacga aggcgccgtt ctcgccgtcc gtgaaggcgg ccgccctcgt
     4741 caccgaggag tgcatgaagc gcggtcttgt ggtgtacccg ggaacgggac agatcggcgg
     4801 cgtggcgggg gatcagttcc tcttcgctcc tcccctggtc gcgacgaaag aggaaatgga
     4861 tatgatgaca gatcgcctcg aagcaggttt gaaggctgcc gcggaggcgc tcaaagggaa
     4921 ggggtaggaa acgccccttt atcttgcacc aaaaaggagg aaattgttca tgaagaaggg
     4981 aattctggca cttgcgctca ttggtatcat ggtgttcgcg tccacaggta tggccgatac
     5041 gttcatcacc atcggcagcg gcggcgtggg cgggacgtac taccccctcg gcggagccat
     5101 ggccgaggtg cttaccaacg ccgggatcgg cgtgcgggca acatcgcgct ccacttccgc
     5161 gtcgcgtgag aactgccgcc ttgtggcgtc ggggcgtgcg catatcggta tgaccatggg
     5221 ttcggcgctc taccaggcga tcaccggcac ggaagcattc gagccggacg gcaagctccc
     5281 cctgcagatt ctcatgagca tgtaccccgc gccgcagcac ctggtaacag tcaagggaag
     5341 cggcatcacc aagcttgagg acatcaaggg caagagagtg tccgtcggcg cgcccggcgg
     5401 cggcgaccag attctgacca acctcattct cgcggcggcg ggaatcgatc ctgaaaagga
     5461 ttttacgaaa cagcagctga ctcagcctga aggcgtcatg gcgctgaagg atggaaacgt
     5521 ggatgcggtc ttctggaact tcgcggcccc cggttcggcg gtgcttgaag tggccgcaca
     5581 gcgcgacgtg gtgatgattc cgcttgagga ggctcttgtg cagaagatca tc
//
LOCUS       DAOTIL010000055         5616 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminobacteriaceae bacterium isolate AMR_MDS_3026
            NODE_17099_length_5616_cov_3.968531, whole genome shotgun sequence.
ACCESSION   DAOTIL010000055 DAOTIL010000000
VERSION     DAOTIL010000055.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868050
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminobacteriaceae bacterium (wastewater metagenome)
  ORGANISM  Aminobacteriaceae bacterium
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae.
REFERENCE   1  (bases 1 to 5616)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5616)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:19:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,224
            CDSs (total)                      :: 3,198
            Genes (coding)                    :: 3,184
            CDSs (with protein)               :: 3,184
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5616
                     /organism="Aminobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17099_length_5616_cov_3.968531"
                     /isolate="AMR_MDS_3026"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:3034609"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..659
                     /locus_tag="P5201_01955"
     CDS             <1..659
                     /locus_tag="P5201_01955"
                     /inference="COORDINATES: protein motif:HMM:NF014684.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HRV97330.1"
                     /translation="TGLLFGFFSGLLVTKIGIPSLITTLATGIIAGGMVYMYTRGVSF
                     YGQMPPSFLALGRGMVGPVPSLIFIMFVVVAVAQVFISKTRTGKYMQATGANQAASRL
                     AGINTDRCKILALSLSGLAAAFTGILLTSRLGSANPEGASGFMMDAFASALLGMTVLS
                     VGRANPFGTFIGALMIGVINNGMTLAGAPYYTQDIVKGLIILLSVTMTSLQAKRMAGN
                     "
     gene            710..1639
                     /locus_tag="P5201_01960"
     CDS             710..1639
                     /locus_tag="P5201_01960"
                     /inference="COORDINATES: protein motif:HMM:NF024799.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter substrate-binding protein"
                     /protein_id="HRV97331.1"
                     /translation="MKKYGVVVAVACLAALLVTGVAAAKQITVGFIATNFSAEAQARV
                     ANAFEKMAKEKNWDVKMLNSTGSIETQSNQLENLYQMKVDAVVMAMAHPLEIRPALDK
                     VLSANIPVITIDSGYVEGVVADITADNFAMGAKASTFLMDSIGGQGNIVVIKFEKHYG
                     TRRRGKVLDVVLSEYPGVKVLAEYSVVATKRFMDDTRAAMETYVTRFGDQIDGVWCAF
                     DQLAYVAGDVLQERGIKAVVVGTDGNQETMRRIGAGQMSATVAQPFEAMAEKAVELVQ
                     AIAAEGKSIEEATGGRKIFYMDAPLVDKTSLPE"
     gene            1721..3166
                     /locus_tag="P5201_01965"
     CDS             1721..3166
                     /locus_tag="P5201_01965"
                     /inference="COORDINATES: protein motif:HMM:NF012589.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FGGY family carbohydrate kinase"
                     /protein_id="HRV97332.1"
                     /translation="MPLFLGVDLGTSSLKAAVLDEKGNIVARAKVPSVMNSPEKDFYE
                     VDAEATWWDGFLSLCTEAGKIVSLREIAAVCVSSVCASFVPVDEGGVPMHDAILYGID
                     KRASRQIERLNRHYGAGLAERLGGMFTTHSILPKAIWLKEERPDAFRRTKYFMEPNNF
                     VTFRLTGERAWDYPSAAAAGLVDREKLTWAEDIFNEQGVSARSFAELRWPLSVLGSIS
                     EDASARTGLPAGIPVITGACDINAEAAAAGALSVGECVVVFGSTVSILLNTERPVRLP
                     GFVSGISVVEGMYRLGAATSSGSRFLQWVHSVIRSGDGKSVSSPTGILMVPWLDGART
                     PFHDPMAKVLFQGMDSGTSGGALLRAGKEALGYELDSLFAMLEQAAPVPETISVSGGL
                     SNDVELMEIVSAITGKKLRLHPSVDAACGDALLAAAGSGYVSLADCGRCARGARTISP
                     DMELHARFRPFVERFRKMTKQRGVPVRSSRG"
     gene            complement(3163..4254)
                     /locus_tag="P5201_01970"
     CDS             complement(3163..4254)
                     /locus_tag="P5201_01970"
                     /inference="COORDINATES: protein motif:HMM:NF016999.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0010468 - regulation of gene expression
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB family transcriptional regulator"
                     /protein_id="HRV97333.1"
                     /translation="MTAFLLLFSVGTAGYLVFKKFALPVPAILGSLFFVSVLNLAGFF
                     PEVQLQWISRYSNIVIGSYVGLRVNKQSLTLLRELPVPALIVSLSMLFLSLAGGSVLY
                     MMSDLSMKTAFLGSTTGGIAEMALLSMSMGADVASVTLLQVFRMLSAILVTPLVCRKW
                     TRWFGAGERSSCSSPSSLSVEPQFSDEEEAAALEPSVAPSAKGYALLAVVSLIGGILG
                     YIFRLPVGILTGAMFAVAATSLLRGEFPPMPNILRTVAQIGIGIIIGSNITISTLHQF
                     GELWLPVVALTAVLLVCSVLLAVLLHRMTGWDYPTCLLSSSLGGLSQMSIISEEMGAD
                     PLKVSILQTIRLLSILIVLPFLFSFLFGA"
     gene            complement(4295..4576)
                     /locus_tag="P5201_01975"
     CDS             complement(4295..4576)
                     /locus_tag="P5201_01975"
                     /inference="COORDINATES: protein motif:HMM:NF012911.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acylphosphatase"
                     /protein_id="HRV97334.1"
                     /translation="MTLIRKRFLVMGTVQGVGFRHFAARMALKFGVTGWVRNLPDGAV
                     EVHAQGEREAMDKMEQWLRKGPSSATVTRLVVLETPLKEGESSFAIRHH"
     gene            complement(4573..5490)
                     /gene="htpX"
                     /locus_tag="P5201_01980"
     CDS             complement(4573..5490)
                     /gene="htpX"
                     /locus_tag="P5201_01980"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: protein motif:HMM:NF003425.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="zinc metalloprotease HtpX"
                     /protein_id="HRV97335.1"
                     /translation="MVSFEGLIAHNKRLSILLFFLLLLLLGGLGASVSYWWGGEWYTG
                     VIVATAVAVIYFLFSWFAGASMILSMSGAREIRHEDAPQLWNVVEELSIAGGLPMPKV
                     YLIEDSAMNAFATGRDPEHASVAITRGLMEKLNREELQAVMAHEMSHVRNFDIRFAML
                     MAVMVGSIVMICDMVFRSVFFGRRRSSRSSDGGKGQAVMAVVALVLAILAPLLATIIQ
                     LAVSRQREYLADASAVELTRNPAGLVSALRKLSGDPNELQRASRGTQHLYIVNPLRKM
                     KQGRNSLFSTHPSLESRIERIEGLSLARA"
BASE COUNT         1150 a         1637 c         1761 g         1068 t
ORIGIN      
        1 cgactggact tctctttggt ttcttctcgg ggctccttgt gacgaagatc ggcattccgt
       61 cgctcatcac cacgctggcc acaggcatta tcgccggggg gatggtgtat atgtacacgc
      121 gcggcgtctc tttctacggg cagatgcccc cttcgttcct cgcgctcggg cgggggatgg
      181 tcgggccggt cccctctctc attttcatca tgttcgtcgt ggtggcggtc gcgcaggtgt
      241 tcatctcgaa gacccgcacg gggaagtaca tgcaggccac gggggcgaat caggccgctt
      301 cccggcttgc cggtatcaac acggaccggt gcaagatttt ggcgctctca ctttccggtc
      361 ttgccgcagc cttcaccggc atcttgctca cctcccgtct cgggtcggcc aaccccgagg
      421 gagcctccgg gtttatgatg gacgcgttcg cctccgcgct gctcgggatg acggtgctca
      481 gcgtgggacg ggcgaatccc ttcgggacgt tcatcggcgc gctgatgatc ggcgtgatca
      541 ataacggcat gacgctcgcg ggcgcgccgt actacactca ggatatcgtg aagggactga
      601 tcatattgct ttcggtgacg atgacctctc ttcaggcaaa aagaatggcg gggaactgac
      661 ctcgccttct tttatagaca ggaaaccaca attcaaggag ggaaagggaa tgaaaaagta
      721 cggggttgtt gttgctgtag cgtgtctggc agcgttgctc gtcacgggag tcgcggcggc
      781 gaagcagata accgtggggt tcatcgccac gaatttctcc gccgaagcgc aggcgcgggt
      841 ggccaacgcg ttcgaaaaga tggccaagga gaagaactgg gacgtcaaga tgctcaactc
      901 gacggggtcc atcgaaacgc agtcgaatca gctggagaac ctctaccaga tgaaggtgga
      961 cgccgtggtg atggcaatgg cgcatccgct ggagatccgt cccgcgctgg acaaggtgct
     1021 ctccgcgaac attccggtga tcacgatcga ctccgggtac gtggagggcg tcgtcgccga
     1081 tattaccgcc gataacttcg ccatgggggc gaaagcttcc actttcctca tggacagcat
     1141 cggcggccag gggaatatcg tcgtcatcaa gttcgagaaa cactacggaa ccaggcgcag
     1201 ggggaaggtg ctcgacgtcg ttctctcgga gtatccgggc gtgaaggtgc tcgccgagta
     1261 cagcgtcgtc gcgacgaaac gcttcatgga cgataccaga gcggcgatgg agacgtacgt
     1321 tacccgcttc ggcgatcaga tcgacggcgt atggtgcgcc ttcgaccagc ttgcgtacgt
     1381 cgcgggggac gtgctgcagg agcggggaat caaggccgtg gtggtcggaa cggacggaaa
     1441 ccaggagacc atgcggagga taggcgccgg acagatgtcc gcaaccgtag cgcagccttt
     1501 cgaggcaatg gcggagaaag ctgttgagct tgtgcaggcc atagcggccg aaggaaaatc
     1561 gattgaagaa gctaccggcg ggcggaaaat attctacatg gatgcgccgc tcgtcgacaa
     1621 gacgtcgctt ccggaatagt tcctttcttc cacgtacgca gcatttcggc gcggatggcg
     1681 atcacatgct ctccgcgccg tttttcagga ggtgtatgca gtgcctcttt ttcttggagt
     1741 ggacctcgga acgtcgtcgc tgaaggcggc cgttctggac gaaaagggga atatcgttgc
     1801 gcgggcaaag gtcccgagcg taatgaactc ccccgaaaaa gatttttacg aggtggacgc
     1861 cgaggcgacc tggtgggacg gttttctttc gctttgtacg gaggcgggaa aaatcgtttc
     1921 tttgcgcgag atcgccgcgg tgtgcgtcag ctccgtctgc gcatcgttcg ttcccgtcga
     1981 cgaaggaggc gttccgatgc acgacgccat tctctacggt atcgacaaac gggcctcgcg
     2041 ccagatagaa cggctcaacc ggcactacgg cgccgggctg gccgagcgtc tcggaggcat
     2101 gttcaccacg cactccatcc ttcccaaggc gatttggctg aaggaggagc gccccgacgc
     2161 gttcaggcgg acgaagtact tcatggaacc gaacaacttt gtcaccttcc ggctgaccgg
     2221 cgaacgcgcg tgggattacc cttccgccgc cgctgccgga cttgtggaca gagagaaact
     2281 gacgtgggcg gaggatattt tcaacgaaca gggcgtttcg gcacgttcct tcgcggagct
     2341 ccggtggccg ctctccgtcc tcggaagcat ttcggaagat gcgtcggcgc gaaccggtct
     2401 ccccgcaggg ataccggtaa tcacaggcgc gtgcgacatc aacgcggagg ccgccgcggc
     2461 gggcgccttg agcgtcggag agtgcgtcgt cgtcttcggc tcgacggtca gcattcttct
     2521 gaacacggag cgccctgtcc ggttgccggg gttcgtttcg ggaatatccg ttgtggaagg
     2581 gatgtaccgg ctcggcgcgg ccacctcttc ggggtcgcgc tttttgcaat gggtgcactc
     2641 cgttattcgg agcggggatg gaaaatccgt atcttctccc accggcattc tgatggtccc
     2701 ctggctggac ggagcgcgga cgccgtttca cgatccgatg gcgaaggtgc tgtttcaggg
     2761 aatggacagc gggacttcgg gaggtgcgct tctgcgcgcc ggcaaggagg cgttggggta
     2821 cgaactcgac tcgctcttcg ccatgctgga acaggcggct cccgtgccgg aaacgatcag
     2881 cgtctcgggc ggtctgagca acgacgtcga gttgatggag attgtatccg cgatcacagg
     2941 aaaaaaactg cgcctgcacc cttcggtcga cgcggcctgc ggcgacgcgc tgcttgcggc
     3001 ggcgggctcg gggtatgttt cgcttgcgga ttgcggtagg tgtgctcgcg gcgcgcggac
     3061 gatctctccc gatatggagc tgcacgcgcg cttccggcct tttgtcgaac gattccgcaa
     3121 gatgacgaaa cagcgcggcg tgccggtacg aagctcccgc ggctaagcgc cgaaaagaaa
     3181 ggaaaaaagg aacggcagca cgatgagaat gctgagcagg cggatggttt ggagtatgct
     3241 caccttcagc ggatcggctc ccatctcctc ggagatgatg gacatctgcg agagccctcc
     3301 gagcgaggag gagagaaggc aggtagggta gtcccacccc gtcatcctgt ggagcagtac
     3361 ggcaagcaga acgctgcaga cgagcaagac cgctgtgagc gcgacaaccg gcagccagag
     3421 ctcgccgaac tgatgaagcg tggagatcgt gatgttggag ccgatgatga tgccgatgcc
     3481 tatctgcgcc accgttcgga gtatgttcgg catcggcgga aactctccgc ggagaagact
     3541 cgtcgccgct acggcgaaca tggctccggt caaaatgccc acgggcagcc tgaaaatgta
     3601 cccgaggatg ccgccgatga gcgacacgac cgccagcaac gcatacccct ttgcggatgg
     3661 cgcaacagaa ggttcgagcg cggcagcttc ctcttcatcc gaaaactgcg gttcaacgga
     3721 aagagaagaa ggggaggaac aactgctcct ctccccggcg ccgaaccatc gagtccattt
     3781 ccggcagacc aggggagtga ccaggatcgc ggagagcatg cggaagacct gaagcagggt
     3841 cacgcttgcg acatcggccc ccatggacat ggagagaagc gccatctccg cgattcctcc
     3901 cgtcgtcgat ccaagaaagg ccgttttcat ggagagatcg ctcatcatat agaggacgct
     3961 tcctcccgcg agggagagga agagcatgct cagcgaaacg atgagcgccg gtacgggaag
     4021 ctcccggagc agcgtgagag actgtttgtt cacccgcaat cctacgtagc ttccgatgac
     4081 gatgttgctg tatcgggaga tccattgcag ctgcacttca ggaaaaaaac cggcaagatt
     4141 aaggacgctc acaaaaaaga gcgagccgag aatcgccggt acgggaagag caaacttttt
     4201 gaataccaga tagccggcgg taccgactga aaaaagcagg agaaaagctg tcataagaga
     4261 aaaccgaacc tccgaaccgc acggtgcgaa ttttttagtg gtgacggata gcgaacgagc
     4321 tctcgccttc cttcagcggc gtttccagca cgaccaggcg cgtcacggtt gcagaagacg
     4381 gtcccttgcg gagccactgt tccatcttgt ccattgcttc acgctcgccc tgcgcgtgca
     4441 cttcgaccgc gccgtccggc agattccgga cccatccggt cacgccgaac ttcagcgcca
     4501 tccgcgccgc aaagtggcgg aaccctacgc cctgtaccgt tcccatcacc agaaaacgct
     4561 tgcggatcaa tgtcatgccc gtgcgagcga aagcccttcg atgcgctcga ttctggattc
     4621 aagggacgga tgcgtgctga aaaggctgtt ccttccctgt ttcattttcc ggagcgggtt
     4681 gacgatgtac aagtgctgcg ttccgcggct cgctctctgg agttcgttcg ggtcgccgga
     4741 cagctttctc aaggcggata ccaggcccgc cggattgcgc gtcagttcga ccgccgaggc
     4801 gtccgcgagg tactcccgct gacggctgac ggcgagctgg atgatcgtgg ccaagagcgg
     4861 cgcaagaatt gcaagcacga gcgccacgac cgccatcacc gcctgtccct tgcctccgtc
     4921 gcttgaacgg ctgctgcggc gacggccgaa gaagacgctg cggaaaacca tatcgcagat
     4981 catgacgatg gagcccacca tcaccgccat cagcatggcg aagcggatgt cgaagttccg
     5041 cacatgcgac atttcgtgcg ccatgacggc ctgaagctcc tcccggttca gcttttccat
     5101 cagcccgcgc gtgatcgcca cgctcgcgtg ctccggatcc cgcccggtgg cgaaggcgtt
     5161 catcgcgctg tcctcgatca gatagacctt cggcatcggg agtcctcctg cgatggagag
     5221 ctcctccacg acgttccaga gctgcggggc gtcttcgtga cggatctccc tcgcgccgct
     5281 catcgagagg atcatggagg cccccgcgaa ccacgagaac aggaaataga ttaccgcgac
     5341 cgccgtggcg acaatcaccc cggtgtacca ttcgccgccc caccagtaac tgacggaagc
     5401 tcccagaccg ccgagcagca gcagcagcaa gaagaagagc aaaatgctca gacgcttgtt
     5461 atgcgcgata agcccctcga agctgaccat gctctcacct ccaggtgacc tgaacggatt
     5521 gcaactcgga agcgtcgtcc gccgcccaca tcgcgcgcgg ctcgtacccc agcctccgcg
     5581 agatcaggtt gttcgggaag acgtcgatag cgatgt
//
LOCUS       DAOTIL010000056         5563 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminobacteriaceae bacterium isolate AMR_MDS_3026
            NODE_17328_length_5563_cov_3.732571, whole genome shotgun sequence.
ACCESSION   DAOTIL010000056 DAOTIL010000000
VERSION     DAOTIL010000056.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868050
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminobacteriaceae bacterium (wastewater metagenome)
  ORGANISM  Aminobacteriaceae bacterium
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae.
REFERENCE   1  (bases 1 to 5563)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5563)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:19:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,224
            CDSs (total)                      :: 3,198
            Genes (coding)                    :: 3,184
            CDSs (with protein)               :: 3,184
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5563
                     /organism="Aminobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17328_length_5563_cov_3.732571"
                     /isolate="AMR_MDS_3026"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:3034609"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..681)
                     /locus_tag="P5201_01985"
     CDS             complement(<1..681)
                     /locus_tag="P5201_01985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014806899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II glutamine amidotransferase"
                     /protein_id="HRV97336.1"
                     /translation="MCGIVGYIGHRNVTDVLVKGLQSLEYRGYDSAGIAVINYPDEEQ
                     TLRTPAVHIVKGVGKVAELKSMLAARPVSGHQGIGHTRWATHGGVTETNAHPHADLSS
                     RLVLVHNGIVENYRELRAELLVPEEQYKSETDTEVIAQLLSALYTGGGAQDMINALVA
                     LHGRLRGSFALVIMVKDIPGKIYCLRKGSPLVVGFSEEGEALCASDVPAILPYTQDVV
                     YLDDGDIAE"
     gene            complement(901..1773)
                     /gene="galU"
                     /locus_tag="P5201_01990"
     CDS             complement(901..1773)
                     /gene="galU"
                     /locus_tag="P5201_01990"
                     /EC_number="2.7.7.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006583363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003983 - UTP:glucose-1-phosphate
                     uridylyltransferase activity [Evidence IEA];
                     GO_process: GO:0009225 - nucleotide-sugar metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UTP--glucose-1-phosphate uridylyltransferase
                     GalU"
                     /protein_id="HRV97337.1"
                     /translation="MTSSISKCLFPVAGLGTRFLPATKEIAKEMLPLVDRPIIEYGVE
                     EALAAGCKEMVMITGRAKKAIEDYFDRSFELEELLKSRNKQTLYDEVISLSEMADFIY
                     LRQHRPLGLGHAVLCGEPVCRNEYFGVILPDDVMVAEPSVLAQLLAVHSERGGSVVAL
                     EEVSAEDTSRYGVVAAENAGNGVFAVKDMVEKPGPEKAPSRLAIMGRYILSPAIFPIL
                     ERQQAGAGGEIQLTDGIRTLISQEPVWGVVYKGRRFDCGTQPGWLHANIRLAMEHPAL
                     KEVVLRAVKEYEEE"
     gene            complement(1799..3169)
                     /gene="glmM"
                     /locus_tag="P5201_01995"
     CDS             complement(1799..3169)
                     /gene="glmM"
                     /locus_tag="P5201_01995"
                     /EC_number="5.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006300902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008966 - phosphoglucosamine mutase
                     activity [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglucosamine mutase"
                     /protein_id="HRV97338.1"
                     /translation="METGEKKVRCLFGTDGVRDVANRGVMTPEMSLRLGRSYILFLTE
                     RGIPRPRIVVGRDTRRSGHMLEAALSAGMASAGAEVFLLGVLPTPGVSFAVKKMSVHG
                     GAVISASHNPAEYNGIKFLDSSGKKLSDDVEGAIEEYMGDNLTDDWRPTGASVGDIHR
                     ATHLLDEYSEWIASLWGEETPFPSAGVIDCAHGAAVETAEKVFRRLGGEWRFIGNRPD
                     GLNINEGVGVMHMAHIAEAVRTNALPIGIAYDGDADRALLTDCRGRVLNGDIILWVLA
                     RWLAERNMLGSGVVATVMSNMALEDHLRKENIPVFRCPVGDRYVMETMSKEGSLLGGE
                     QSGHIIISSFVNTGDGLCTGLFFLKACLELGEDISTLADRFAPYPQILRNVEVSRDGK
                     VSGDFLRDLSSEAETLLQGAGRILIRPSGTEPLMRILVEARDEKLMEHVSEKLVNAIK
                     EQCRYQ"
     gene            complement(3201..4427)
                     /locus_tag="P5201_02000"
     CDS             complement(3201..4427)
                     /locus_tag="P5201_02000"
                     /inference="COORDINATES: protein motif:HMM:NF019561.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CdaR family protein"
                     /protein_id="HRV97339.1"
                     /translation="MSESGKIDAILASPLFLKIMAGVIALALWFYVSGNRSTETTRTL
                     VVPLEYLNVSPQTTLKTAVQEVEIVLSGAQYVLSAIPQDGVVCEVDLRGVGVGKYRIP
                     VRSIVPKDVKIMDVKPTHVDVELARFIDRLIPVEIAVDKGLPPGLFLDMVEILPKNVS
                     VKGSERDIAKIDAVKIVPTLDELKAGGEHTLAVEVVKSEAFEDEVVVEPKTVKLSAVL
                     AEGVPKKSVPVNARIIGEPGGDFKLKAVVVEPAVVTVEGPLAKLNAVTRVDTETIDLT
                     GISKEQNIVIPLRAPEDQMVTIVGAASVRINVLLTPFTVTRLLSRLNVEVDGRSVYPG
                     WKVEPNIVNVTVEGVPSEVNALDEKDVVIRPFVNVTNVVSRRLTVPVQIQNKSGGKLK
                     VVKIDPASVTVIADIR"
     gene            complement(4453..5265)
                     /gene="cdaA"
                     /locus_tag="P5201_02005"
     CDS             complement(4453..5265)
                     /gene="cdaA"
                     /locus_tag="P5201_02005"
                     /EC_number="2.7.7.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013048136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004016 - adenylate cyclase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diadenylate cyclase CdaA"
                     /protein_id="HRV97340.1"
                     /translation="MFDTLRWQDFLDILVIALIVHRLLLLIVGTRAMQLVKGLLILGV
                     VAAAARLLDLRTLSWFLGQTLGILLIAIPIVFQPELRKMLEELGRGGGLWRRKRAAEK
                     AEAFSHEVSRAIGYLKGQKIGALIVLERNTGLKDFWRTAIKLNSEISQELLITIFWEG
                     TPLHDGAVILDRERIIAASCYLPLTENSDLSRWIGTRHRAALGVTEVSDAIALVVSEE
                     RGEVSLAINGRLSKNLKEAQVHKLLSHYFSGEEAEHKPLMERLQEDLRALWS"
     gene            complement(5331..>5563)
                     /locus_tag="P5201_02010"
     CDS             complement(5331..>5563)
                     /locus_tag="P5201_02010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006300899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="alanine/ornithine racemase family PLP-dependent
                     enzyme"
                     /protein_id="HRV97341.1"
                     /translation="ERLRAIVAVGRQDVLIEGLTPEIPEAVILGGSSDHMIIDVEDVR
                     PAPELGGILRFFPNYGAMLALSTSPYIAFDVV"
BASE COUNT         1143 a         1701 c         1529 g         1190 t
ORIGIN      
        1 ctcggcgata tccccgtcgt caagatagac gacgtcctgc gtatacggaa gtatggcggg
       61 cacatccgaa gcgcagagcg cttccccttc ttccgaaaag ccgacgacca gcggcgagcc
      121 tttgcgaagg cagtagatct tcccgggaat gtctttcacc atgatgacaa gcgcaaacga
      181 acctctcaag cgtccgtgga gggcgacgag ggcattgatc atgtcctgtg ctccgccgcc
      241 ggtataaagg gcggagagaa gctgcgcgat gacctccgtg tcggtctccg acttgtactg
      301 ttcctcggga acaagaagct cggcgcgaag ttcacggtag ttctccacta ttccgttatg
      361 gacaagcacc aacctggagg aaagatctgc gtgaggatgg gcgttggttt cggtgacgcc
      421 cccgtgggtt gcccaccggg tatgccctat cccctgatgc ccggaaacgg gacgggcggc
      481 gagcatggac ttcagctcgg ccactttgcc gacgcccttg acgatatgca ccgcaggcgt
      541 ccggagagtt tgttcttcgt cagggtagtt gataacggcg atccccgccg agtcataccc
      601 tctgtattca agggactgaa gtccttttac aagcacatcc gtaacatttc ggtggccaat
      661 atagccaacg attccgcaca ttatacagca ccgcctttgt atccactcac gagagactgc
      721 gtttcacttt gtcccggaaa ttactttccg gttttgcaga aaggctttgc gggccggtct
      781 ctcacggccc cggaggcatc cgccgacaga tcgatactcc tccgtcctcg tcagccgaag
      841 actcgattcc ccggatccgg ggagctgttc tccggcgcag gcgcttcgtt catgcgctgt
      901 ctactcttct tcgtattcct tcaccgctcg cagcaccacc tccttcaatg cagggtgctc
      961 catggcaagg cgaatgttgg cgtgcagcca tcccggctgc gtcccgcagt cgaaacgcct
     1021 tcccttgtac acaacgcccc agacaggctc ttggctgatc agcgttcgaa tgccgtcggt
     1081 cagctgaatc tctcctcccg ctcccgcctg ctggcgctca agaatcggga agattgccgg
     1141 agagagtatg tagcgtccca tgatagcaag cctgctcggg gctttttccg gtcccggctt
     1201 ctccaccatg tcctttactg cgaaaacgcc gttcccggcg ttctcggcgg caacgacgcc
     1261 gtaacgcgac gtgtcttccg cagagacttc ttcaagagcg acgacgctgc ctcctcgctc
     1321 gctgtgcact gcaagaagct gcgcaaggac cgagggttcg gcaaccataa cgtcgtcggg
     1381 aaggatgacg ccgaaatact cgttgcggca gaccggttcc ccgcagagca ccgcatgccc
     1441 caggccgaga ggacgatgct gccggaggta gatgaaatcc gccatctcgg agagcgagat
     1501 tacctcgtcg tagagggtct gcttgttccg tgacttaagc agctcttcaa gttcaaaaga
     1561 gcggtcgaag tagtcttcga ttgccttctt tgcccggccg gtgatcatga ccatctcttt
     1621 gcatcccgcg gcaagcgctt cttcaacgcc gtattcaatg atgggtctgt cgacgagcgg
     1681 cagcatttcc tttgcgatct cttttgtcgc ggggaggaaa cgcgttccca gtcccgccac
     1741 ggggaagagg cattttgata tcgaagaagt catacggcaa ccacctttca cacgatcgtt
     1801 attgatagcg gcactgctct ttaattgcgt tcacgagctt ctcggaaacg tgctccataa
     1861 gcttttcatc ccgcgcttca acaaggattc gcatcagagg ctccgtgccc gaggggcgga
     1921 tgagaattct tccggcgccc tgaagcagcg tctccgcttc ggaggagagg tccctcagga
     1981 agtcgccgct caccttcccg tcgcgggaaa cttcgacgtt gcgcagaatc tgcggatacg
     2041 gggcgaaacg gtctgcgagg gtcgaaatat cttcaccgag ctccagacac gccttgagga
     2101 agaaaaggcc ggtacagagt ccgtcgccgg tgttcacaaa cgaggagata atgatgtggc
     2161 cggactgttc tcctccgagc agcgatccct ccttggacat cgtctccata acgtatcggt
     2221 cgcccacagg gcagcgaaaa acagggatat tttctttccg gagatgatct tcgagcgcca
     2281 tgttgctcat taccgtggca acgactcccg agccgagcat attgcgctcg gcgagccagc
     2341 gcgcgagtac ccagaggatg atatcgccgt tgagtactct ccctctgcaa tccgtcagca
     2401 gcgcgcggtc ggcgtctccg tcgtaggcga tgcctatcgg gagcgcgttc gttctcaccg
     2461 cttcagctat gtgcgccatg tgcataacgc cgacgccttc gttgatattc agtccgtcgg
     2521 gccggtttcc aataaagcgc cactcgccgc cgagcctgcg gaagactttt tcagccgttt
     2581 ccaccgccgc gccgtgcgcg cagtcgataa cccccgccga cgggaagggg gtctcttccc
     2641 cccagagcga ggcaatccac tcggaatatt catcaagcag atgcgtggct ctatgaatat
     2701 ctcccacgga agcgccggtg gggcgccaat cgtcggtgag gttatccccc atgtactctt
     2761 cgatcgcccc ctcgacgtcg tcggaaagtt ttttcccgga tgaatcgagg aattttattc
     2821 cgttgtactc cgcagggttg tgggaggcgc tgatcaccgc tccgccgtga accgacatct
     2881 tctttacggc gaaactgacg cccggagtag gcaacactcc gaggagaaag acctccgcgc
     2941 cggccgaagc catgccggcg gaaagagcgg cttccagcat gtgcccggaa cgcctggtgt
     3001 cccttccgac gacgatcctt ggacgaggga tgcccctctc cgtcaggaaa agaatgtagg
     3061 agcgaccgag ccgcaatgac atctcgggag tcatcactcc cctattggcc acgtcccgca
     3121 caccatcggt cccgaaaaga caacgtactt ttttctcacc agtttccatg cagcctcacc
     3181 atccccgccc gtataagaga ttacctgata tccgcaatta cagtgacgct cgcaggatca
     3241 atttttacta ccttcaattt accgcccgac ttgttctgaa tctgcacagg aacggtcaag
     3301 cgtctggaaa cgacgttggt gacgttgaca aaggggcgta tcaccacgtc tttttcgtcg
     3361 agtgcgttca cttcggaggg gactccctcc acagtgacgt tcacaatgtt cggctccact
     3421 ttccacccgg ggtagacgct tcgcccatcg acctcgacgt tcagccggga gaggaggcgg
     3481 gtgacggtga agggggtcag aagaacgttg atccgcaccg aagcggcgcc gacaatggtc
     3541 accatctgat cctccggagc gcgcaacggg atgacgatgt tctgctcctt ggagatccca
     3601 gtcaggtcga tcgtctcggt atcaacccgc gtcacggcgt tgagtttcgc cagaggcccc
     3661 tcgactgtga cgacggcagg ttcaacgaca acggccttca gtttgaagtc gccgcccggt
     3721 tcgccgataa tccgggcgtt caccgggacg ctcttcttcg gaactccctc ggcgagcacc
     3781 gcgctgagct tgacggtttt cggctccaca acaacctcat cttcaaacgc ctccgacttg
     3841 acgacctcga cggcaagcgt atgctcccct cccgccttca gctcatcgag ggtgggaact
     3901 atcttgacgg cgtctatctt cgcgatatcg cgttcgctcc ccttgacgct gacgtttttg
     3961 ggaagtatct cgaccatatc gagaaaaagc cccggaggaa ggcctttgtc gacggcgatt
     4021 tccaccggaa tgaggcggtc gataaagcga gcgagttcca cgtcgacgtg cgtaggtttg
     4081 acgtccatta tcttgacgtc cttgggaacg atggaacgga caggtatccg gtattttccg
     4141 acgcccactc ccctgaggtc gacctcgcag accacgccgt cctgaggaat ggcggagaga
     4201 acatactgcg cgcccgagag gactatctcc acttcctgca cagcggtttt gagcgttgtc
     4261 tgcggcgaga cgttgagata ctcgagagga acaaccaatg tcctcgtcgt ctcggtcgat
     4321 ctgttgccgg agacgtaaaa ccagagagcg agcgcgatca ccccggccat tattttcagg
     4381 aacagcggcg acgccaggat cgcgtctatc tttccggatt ccgacataag cgcctgcgca
     4441 ccttcttccc gttcatgacc acagcgcccg cagatcttcc tgcaggcgtt ccatgagtgg
     4501 tttatgttca gcttcttcac cgctgaaata gtgggagagc agtttatgca cctgggcctc
     4561 cttcaggttt ttggaaaggc ggccgttgat tgcaagcgaa acttctcccc gttcttcgga
     4621 gacgacaagg gcgatcgcgt cggaaacttc cgtcacgccg agcgccgccc tgtgacgggt
     4681 gccgatccat cgggacagat cggagttttc cgtgagcggc aaatagcagc ttgcagcgat
     4741 gatcctctcg cggtcgagga tcaccgcccc gtcgtgcaaa ggcgttccct cccagaagat
     4801 ggtgatgaga agctcctggc ttatctccga attcagctta atggcagttc tccagaagtc
     4861 cttgagcccg gtgttgcgtt cgaggacgat aagcgcgccg atcttctgcc ctttgagata
     4921 accgatcgcc ctggatactt catggctgaa ggcctccgcc ttttcagcag ctcgtttccg
     4981 cctccagagg ccgccccccc tgccaagctc ttcgagcatt tttcgcagct ccggctgaaa
     5041 gactatcgga atggcgatca ggaggatacc cagcgtctgc cccaaaaacc atgagagcgt
     5101 ccgcaggtcg agcaatcttg ccgccgcagc aacgacgccg aggatgagaa gccccttgac
     5161 gagctgcatc gcacgagtgc ccacgatcag cagaagcaat ctgtgcacaa tcagggcgat
     5221 gaccagtata tccaggaagt cctgccatct cagagtatca aacatataca atcgccctcc
     5281 ggggcggaga agtatcaggg aacgcgcccc tgcggacagg ggcacgtata ctataccacg
     5341 tcgaaggcga tatacggcga cgttgaaaga gcgagcattg ctccgtagtt ggggaaaaag
     5401 cgcagtatcc ccccaagctc cggcgcagga cgcacatctt cgacgtcgat aatcatatga
     5461 tcgcttgatc ctccgagaat gaccgcttcc ggaatttccg gcgtcagccc ctcgatgagg
     5521 acatcctggc gccctaccgc gacgatcgct cgcaagcgct ccc
//
LOCUS       DAOTIL010000057         5553 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminobacteriaceae bacterium isolate AMR_MDS_3026
            NODE_17371_length_5553_cov_3.191524, whole genome shotgun sequence.
ACCESSION   DAOTIL010000057 DAOTIL010000000
VERSION     DAOTIL010000057.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868050
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminobacteriaceae bacterium (wastewater metagenome)
  ORGANISM  Aminobacteriaceae bacterium
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae.
REFERENCE   1  (bases 1 to 5553)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5553)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:19:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,224
            CDSs (total)                      :: 3,198
            Genes (coding)                    :: 3,184
            CDSs (with protein)               :: 3,184
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5553
                     /organism="Aminobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17371_length_5553_cov_3.191524"
                     /isolate="AMR_MDS_3026"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:3034609"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1028)
                     /locus_tag="P5201_02015"
     CDS             complement(<1..1028)
                     /locus_tag="P5201_02015"
                     /inference="COORDINATES: protein motif:HMM:NF018684.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysozyme inhibitor LprI family protein"
                     /protein_id="HRV97342.1"
                     /translation="MKFAVVSVLCLLLLCGAGWGGEHPLDVEIQECMDKDPSTHGTIK
                     CAREGAKKWDTELNRAYKELMGLLPKQGQDALRTAQRAWIPWRDREFGLVGSVYGAIY
                     NSLDGGTMWLVVNAIAEMEVVRGRTLELIAWSEEMKKGKPVFEGAYPEKQTDDQLAAA
                     MRVKNASIRLGKRIGKEGEKAAAANLAAWEEFRNRNVLFMASFYGKQREKSFPLHARM
                     LMNNDRVKRLEGLLEDLNQNGLEESSGQEETGQKDALPKEPFQGDRSLYLPAEGQCDF
                     KAYIIDPDPKGANVRDAPGGKIIATMPHRPDDPALITVSVTGHKNKWLSVLLHDGRKG
                     WVFGELVGV"
     gene            complement(1047..1772)
                     /locus_tag="P5201_02020"
     CDS             complement(1047..1772)
                     /locus_tag="P5201_02020"
                     /inference="COORDINATES: protein motif:HMM:NF019844.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0070064 - proline-rich region binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SH3 domain-containing protein"
                     /protein_id="HRV97343.1"
                     /translation="MRVVKICAVLASILLLSVPLAANPTVESFEWPKSGMLVGERVNL
                     RSGPATSSKVIGRFVDEYEGGELLVTGRSKGREEFPWYRVVSHKFGEGWLYGKFIAVE
                     EPEDPVRRYALKIREDFGLSPAQAVKMYGEPMKRSREKLHIPDFNVTAEIVTLEFHGH
                     TAIYWDGVLQSVEIPAGFMGFADILLGMDVEEAASRLGTPILRQEDTLIFGFERDEIA
                     LTEGKTGGRTVVAGLAYRRVVYD"
     gene            complement(1806..3761)
                     /locus_tag="P5201_02025"
     CDS             complement(1806..3761)
                     /locus_tag="P5201_02025"
                     /inference="COORDINATES: protein motif:HMM:NF021489.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2207 domain-containing protein"
                     /protein_id="HRV97344.1"
                     /translation="MRKCLWILVFACLVWGFAAAPLFAEERILRFDVEATVEEGASLI
                     VTERIAFVAEGNDIKRGLYRTFPTDFTDTEGVRRRAGFDLLSAELDGAPTPSLVERVG
                     ENLDIRLGDLEKLLAHGEHQFTITYRTSGQLGFFGEHDELYWNVTGNEWAFPIEKVSF
                     RLRLPGRDFGADFSSIEFYTGKKGERGQDAFVTKEGTVESTRLLSQGEGLTVVYTWPK
                     GIVAPPAEPAPVLEKWTPSPYRVAHLAMPVVLALVMTLLWILWGKDPPAKAVFPRFAP
                     PQGIEAGFSRYVWSMRMDDQAFAAMVLGMAVKGPLTIEERSLVAEAAKQTGKDASQAG
                     MGMKLLSKLDGKSYVLRLNREKLSNTNLTIDERVLVDELFGSTRSDIHLSSADRPVIQ
                     DAFGQLGKRFKERAKPLLKTNTGKWLAGVALFEIYAVVMLVLMILSGEGRLEPVLALM
                     AGPFLLLPFAIPVPSGKGSMMSKFFLRVFFPGIFLFVTAGAVLAGSTSGIEVDLLSVP
                     GPFLCVLILLLFKPLMRVRSEEGARLNEELEGLRMFMTAAEKHRLELGNPPEETPKLF
                     EELLPYAFALDVAETWANRFENILAAAHYTPSWYRGDMHTFSQTAGVAAFASGFAGAV
                     ASGTRSSGRGGSGSSGGGGGGGGGRGW"
     gene            complement(3839..5527)
                     /locus_tag="P5201_02030"
     CDS             complement(3839..5527)
                     /locus_tag="P5201_02030"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRV97345.1"
                     /translation="MKKRVSAVVWALLLAVLVVSPVSANTLNALPAWPDSTLYGVANI
                     EEPKEFINALVDSKLFKMAAAMEQDMQMAADWLKQFPVQSASVVVGLTDKGFSLQGAA
                     RVDESKKELLQKLAEGKGEEGDIDLLINSPVPGALILAPVEGNVYAVVADGVAGVLLS
                     VEGDMVLFGFGPEDIEAAKAALADPAKRMNMQRRLPQKNFYFFHDNGMAAAELIAQSK
                     GTLEEPVENLFAEIGFGLTEKGFDLSVFTNFAKTFGLDKLETEIRPLSKDDLFLVGGG
                     SAWLTMAGRVVMEKKHFDMIREAAEGGDQDAAQVVEVLEQLKVMGLTENALISILKSV
                     GIVLGGESSFSGMPIPGGYLYTSGEKDWVELLLPLLEAIVKESGGEFEPLTLPGWQAL
                     YVMRDPVDVVLGIRDGIALAGFLSPESLEKAPELSQGAENLLGGNNSMLFYLDADVVR
                     RIVVQLFDPENPIAALFLADDDDFLEAAPFLFEGLKATAGFKGIQMNFSGMERLDFAM
                     LFDTPNSEIIAALDGLAAKWQEFVETQEEEEEAEEEEAEEEEKIEEEAKEAPKS"
BASE COUNT         1187 a         1733 c         1394 g         1239 t
ORIGIN      
        1 acgcccacca gttcgccgaa tacccatcct tttcttccgt cgtggaggag aactgagagc
       61 cacttgttct tatgccccgt aacgctgacc gtaatgagag cgggatcgtc aggccggtga
      121 ggcatcgtgg cgatgatctt tcctccggga gcgtcgcgga cgttggcccc cttgggatca
      181 gggtcgatga tatacgcttt gaagtcgcat tgtccctctg ccggcagata gagcgatcta
      241 tctccttgga aaggttcttt gggaagcgca tccttttgtc cggtctcctc ctgtcctgac
      301 gattcctcca acccattctg gttcagatct tcgagcaatc cttcgagacg cttcacccgg
      361 tcattgttca tcagcatgcg cgcatgcagg gggaaacttt tttccctttg cttcccgtag
      421 aaggaggcca tgaagagcac attcctgttc ctgaactcct cccacgccgc aaggtttgcc
      481 gcagcagcct tctctccctc tttcccgatt cgttttccca ggcggatact tgcgttcttc
      541 accctcatcg cggcggcaag ctgatcatcc gtctgttttt ccggatacgc gccctcgaaa
      601 accggcttcc cttttttcat ttcctccgac catgcgatga gttcgagcgt tcttcccctg
      661 acgacctcca tctccgcgat agcgttgacg acaagccaca tcgtgccacc gtcgagactg
      721 ttgtagatcg ctccgtacac cgaacccacc agtccgaact ccctgtctct ccaggggatc
      781 caggcccgct gggccgttcg cagggcatcc tgtccctgtt ttggaagcag ccccatcagc
      841 tctttatatg ctctgttgag ctccgtatcc cacttcttcg ccccttcccg ggcgcacttg
      901 atcgttccgt gggtgctcgg atccttgtcc atacactcct ggatctcaac atccagaggg
      961 tgttcgcccc cccacccggc gccgcacagc aggagcaggc agagtaccga cacaaccgcg
     1021 aacttcatga aaccccacct ccatcttcaa tcatagacaa cccggcggta ggccaacccg
     1081 gcgacgaccg tcctgccgcc cgtctttccc tccgtgagcg ctatctcgtc gcgctcgaag
     1141 ccgaaaatca gcgtatcttc ctgccgcaga ataggcgtac cgagccgtga agccgcttct
     1201 tccacgtcca ttccaagaag aatatccgcg aaccccatga atccggccgg tatctccacc
     1261 gactggagca caccgtccca atagatcgcg gtatggccgt ggaattcaag cgtcacgatc
     1321 tccgccgtca cattgaagtc ggggatatgc agtttctcac gagaacgctt catcggttcg
     1381 ccgtacatct tcacggcttg agcaggagag agcccgaagt cctcgcggat tttcagcgcg
     1441 taacggcgca ccggatcctc gggttcctcg acggcaatga atttgccata caaccaccct
     1501 tctccgaatt tgtgagagac tacacgatac cacgggaact cttcgcgacc tttggatctc
     1561 cccgtcacca agagttcgcc cccctcgtat tcgtccacga aacggccgat gaccttcgat
     1621 gaagttgccg gccctgaacg aagattcacc ctctccccaa ccagcatgcc cgactttggc
     1681 cattcgaagc tctccaccgt cggatttgcg gccaacggaa cagagagaag gaggatactc
     1741 gccaaaaccg cgcagatttt tactactctc attatccctt ccctcctttc gataaagaaa
     1801 gaatactacc agccccgtcc gccgcctccg ccgcctcctc ctcccgagga gccgcttccg
     1861 cctctccccg aggagcgcgt tccggacgcg actgcgcctg caaaacctga agcgaacgcg
     1921 gcgacgccgg ccgtctgtga gaaggtatgc atatcccctc tgtaccacga cggcgtatag
     1981 tgcgcggcgg caagaatgtt ctcaaaacgg ttcgcccacg tctcggcgac gtcgagggca
     2041 aaggcgtagg gaagaagctc ctcaaagagc ttcggcgtct cttcaggagg gtttcccaat
     2101 tcaagccggt gtttctctgc tgccgtcatg aacatgcgaa ggccttcaag ctcctcgttc
     2161 agtctggcgc cttcctcgga gcggacgcgc atcaggggtt tgaagagaag cagaatcagc
     2221 acacagagga acggaccggg aaccgagagg aggtcgacct cgatcccgct ggtcgaaccc
     2281 gccagcacag cccctgccgt aacgaagagg aagatgccgg ggaaaaaaac tctgaggaaa
     2341 aatttcgaca tcatacttcc ctttcccgac ggaacgggaa tggcgaaagg aagcaggaga
     2401 aacggtccgg ccatcagagc aagcacaggt tcaagacgcc cctcgccgga gaggatcatg
     2461 agaacaagca tgacgacggc gtagatctcg aaaagagcga ctccagcgag ccactttccg
     2521 gtgttggtct tcagaagcgg ttttgccctc tccttgaagc gtttcccaag ctgtccgaaa
     2581 gcatcttgaa ttacaggacg gtccgcgctt gagagatgga tgtcgcttcg ggtggagccg
     2641 aaaagctcgt cgacaagaac ccgttcatcg atggtgaggt tggtattcga gagcttctcc
     2701 ctgttcaggc gaagaacata ggattttccg tcaagctttg aaagcagctt catacccata
     2761 cccgcctgcg atgcatcttt tcccgtctgc ttcgccgcct ccgcaacaag agaacgctcc
     2821 tcgatcgtca agggtccctt gacggccatc cccagaacca ttgcggcgaa cgcctggtcg
     2881 tccatgcgca tcgaccatac gtagcgggaa aaaccggcct cgattccctg aggaggggca
     2941 aatcttggga atactgcttt tgcgggggga tctttgcccc acagtatcca gagcagcgtc
     3001 atcaccaggg caagaacaac gggcattgca aggtgcgcta ctctgtacgg ggaaggcgtc
     3061 catttttcca gaacgggcgc gggttcagcc ggaggggcga ctatgccctt cggccatgtg
     3121 tagactactg tcaagccctc gccttgcgaa agcaaccgag tagactccac cgtcccttct
     3181 ttcgttacga atgcatcctg cccccgctct cccttttttc cggtatagaa ctctatgctc
     3241 gaaaaatcag cgccgaaatc ccttccgggg agtcgtagac gaaatgatac cttttctatc
     3301 gggaacgccc attcatttcc tgtgacgttc cagtacagtt cgtcgtgttc cccgaaaaaa
     3361 ccgagctgtc ccgatgtcct gtatgttatt gtaaactgat gctcgccgtg agcaaggagt
     3421 ttttccaggt cgccgaggcg aatatcgagg ttttctccga cacgttctac caacgatggg
     3481 gtcggcgccc cgtcaagctc cgcggaaaga agatcaaaac cagcccttct gcgaacgcct
     3541 tccgtatcgg taaaatcagt cgggaaagtt ctgtagagtc cacgctttat gtcgtttccc
     3601 tccgccacaa aagcgatgcg ctctgttacg ataagggagg ctccctcttc caccgttgct
     3661 tcgacgtcga aacggagtat tcgctcctct gcaaaaagcg gcgctgctgc aaaaccccat
     3721 accagacacg cgaatacaag tatccataaa cattttctca tggcgttcca cctccttgca
     3781 tgggagagga aaaagaagct ccgaggtcat atccccggag cttcttgaaa ttcgtgaact
     3841 acgacttcgg cgcttccttc gcttcctctt ctatcttttc ctcttcttct gcttcttcct
     3901 cttctgcttc ttcttcctct tcttgagtct ccacaaactc ctgccatttt gcggcaagtc
     3961 cgtcgagagc ggctattatc tcagaattcg gcgtatcgaa gagcatcgcg aaatcgagac
     4021 gctccattcc gctgaagttc atttggatac ccttgaatcc ggctgtagcc ttcaatccct
     4081 cgaaaaggaa cggagcggcc tccaggaagt cgtcgtcgtc agcgaggaag agcgcggcga
     4141 tcgggttctc cggatcgaaa agctgcacca cgatacggcg aacaacatcc gcatccaagt
     4201 agaaaagcat agaattgttc ccgccgagga gattctccgc accctgactc aattccggcg
     4261 ccttctccag gctttccggg ctgaggaatc cggcgagcgc gataccgtcg cgaataccaa
     4321 ggacgacatc cacaggatcc ctcattacat aaagcgcctg ccaccccggg agcgtcaggg
     4381 gctcgaattc tccgccgctc tctttcacta tggcttcgag gagcggcagt aggagctcaa
     4441 cccaatcttt ttcaccggag gtgtacaggt acccgccggg aataggcata ccggagaagg
     4501 aagattctcc gccgaggact atgccgacgg acttgaggat ggagatgagc gcgttctctg
     4561 taagtcccat cactttcagc tgttcgagca cttccacgac ctgcgcagca tcctggtccc
     4621 cgccttctgc ggcttcacgg atcatatcga agtgcttctt ctccatgaca acccgcccgg
     4681 ccatcgtcag ccatgcgctt ccgccgccga cgaggaacag atcgtctttt gagagcgggc
     4741 gaatttccgt ctccagcttg tccaatccga acgtcttggc gaagttggtg aacacggaga
     4801 gatcaaaacc cttttctgta aggccgaagc cgatctccgc aaagaggttt tctaccggtt
     4861 cttccagcgt ccccttggac tgcgctatga gttcggcagc cgccattccg ttgtcatgga
     4921 agaaatagaa gtttttctgg gggaggcgcc gctgcatgtt catacgtttc gccgggtcgg
     4981 cgagcgccgc ctttgccgcc tcgatatctt cgggaccgaa accgaacagc accatatctc
     5041 cctcgacgga gaggagaacg cccgcaactc cgtcggccac aaccgcatag acattcccct
     5101 cgaccggagc gagaatcagc gcgccgggaa ccgggctgtt gatcaggaga tcgatatccc
     5161 cttcctctcc cttgccttcg gcgagctttt gaagcagctc ttttttgctt tcgtcaaccc
     5221 tggcggcgcc ctggagggag aatcctttgt cggtaaggcc aacgacaaca gacgccgact
     5281 gcacagggaa ctgtttcagc caatccgccg ccatctgcat atcctgttcc atagcggccg
     5341 ccatcttgaa gagcttggaa tcgaccagag cgttgatgaa ttccttcggc tcctcgatat
     5401 tcgctacccc gtagagcgtg ctgtcgggcc atgccggaag tgcgtttaag gtgttcgcgc
     5461 tcaccggaga tacaacaaga accgctaata gcaacgccca tactactgcc gaaactctct
     5521 ttttcatagg aacccctcct cgatgggaac gaa
//
LOCUS       DAOTIL010000058         5493 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminobacteriaceae bacterium isolate AMR_MDS_3026
            NODE_17597_length_5493_cov_4.770136, whole genome shotgun sequence.
ACCESSION   DAOTIL010000058 DAOTIL010000000
VERSION     DAOTIL010000058.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868050
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminobacteriaceae bacterium (wastewater metagenome)
  ORGANISM  Aminobacteriaceae bacterium
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae.
REFERENCE   1  (bases 1 to 5493)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5493)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:19:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,224
            CDSs (total)                      :: 3,198
            Genes (coding)                    :: 3,184
            CDSs (with protein)               :: 3,184
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5493
                     /organism="Aminobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17597_length_5493_cov_4.770136"
                     /isolate="AMR_MDS_3026"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:3034609"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..249
                     /locus_tag="P5201_02035"
     CDS             <1..249
                     /locus_tag="P5201_02035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013047730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HRV97346.1"
                     /translation="PEMQLALSNAYYDSAAWPWLYEPSLLRAMEAAGVLRKLFYGSDF
                     PILGYPKYEKMIAASGVERGEVEKFLHHNAFDFMAKRC"
     gene            301..375
                     /locus_tag="P5201_02040"
     tRNA            301..375
                     /locus_tag="P5201_02040"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:333..335,aa:Val,seq:tac)
     gene            399..475
                     /locus_tag="P5201_02045"
     tRNA            399..475
                     /locus_tag="P5201_02045"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:433..435,aa:Asp,seq:gtc)
     gene            492..567
                     /locus_tag="P5201_02050"
     tRNA            492..567
                     /locus_tag="P5201_02050"
                     /product="tRNA-Phe"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:525..527,aa:Phe,seq:gaa)
     gene            590..664
                     /locus_tag="P5201_02055"
     tRNA            590..664
                     /locus_tag="P5201_02055"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:622..624,aa:Gly,seq:gcc)
     gene            681..754
                     /locus_tag="P5201_02060"
     tRNA            681..754
                     /locus_tag="P5201_02060"
                     /product="tRNA-Cys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:713..715,aa:Cys,seq:gca)
     gene            766..841
                     /locus_tag="P5201_02065"
     tRNA            766..841
                     /locus_tag="P5201_02065"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:799..801,aa:Asn,seq:gtt)
     gene            1002..1166
                     /locus_tag="P5201_02070"
     CDS             1002..1166
                     /locus_tag="P5201_02070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013048626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRV97347.1"
                     /translation="MFKKLVYMAGKPFWLLPEGSLLDVEQRNGRIVDPVVFRKKNDDR
                     INENTHLSMN"
     gene            1418..5227
                     /locus_tag="P5201_02075"
     CDS             1418..5227
                     /locus_tag="P5201_02075"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HRV97348.1"
                     /translation="MDQMQSGKSRNNDRTVQSVSALLCSISAAMSLFNAINKTYFNAV
                     LVFLCLLLLTFFYAYYRRTKNFSAWSTLFLLVSGSMVVYLFYQGGTKGAGLLWAYIFP
                     FLAFQFKGYKAGLRFVAVLYAVLAGVFASALTGHAPLHFSPLFMIVYFSVHITISGFL
                     FFYEKRRTITERIIFESREKYKALFDNFSVGVAMISPEFELLELNGIARKWFDRSPEK
                     QHSFCYEFLDSEQKLGVCEDCPLHSVMESKHTATQYRKKMTVDGIRNFKIVCSPMFDK
                     EGNVSAVIETLEDVTEIQRAREFLEESENRFRTLFEQVPFISVQGYDKERNVIFWNSA
                     SELLYGYPAKEALGKRLEDLIIPAEKIQEHVEAISAWIQGGPPLPPSEVMLKRADGSS
                     VHVFSSHVLIRNVRGEKEFYAIDIDITERKRLEEHLSSNEEKYRLLVETAQEAICVAQ
                     DERLVYFNPTVSDMLGYSDSELSSMHFLEMVYEDDRELLLQNYRKRLAGSGVEQSYEI
                     RLLRKDGTVAWVELKATLIEWNGSPATLVFLNDITTQKAALRELMETNRRLELAIERT
                     REFATKADAANRAKSDFLANMSHEIRTPMNGVLGMAGLLLDTELNREQRRCAEAVRAS
                     AESLLGVLNDILDFSKIEAGKLDLERLRFDLSVMLDDFAAGMAVRAHQKGLEFVCAPD
                     PEVPSRLLGDPGRLRQILTNLVGNAIKFTSKGEVVVRVSLEEESEQSALLRFSVRDTG
                     IGVPDEIKESVFEKFIQGDSTTTRRYGGTGLGLSISKQLVEMMGGRIGVESPPKLSAE
                     NGLSHWSEAGPGSDFFFTVRCEKQPSEVESGIVVQGELSGVRVLIVDDNATNREIVRA
                     HLVSRGMRPFEAQDGASALRLLEEAVEQGDPAALALVDMNMPEMDGETLGRKIREDGR
                     FSQTRLVMLTSLADRGDAKRFAEAGFSAYLPKPVRARELLGVLALVHARGEDVSPDFF
                     ATRHSLNELSSAHPRPFAGKGYRILVVEDNSTNRQVALGILGKFGVEACSVENGEKAV
                     EEVGRTAYDLLLMDVQMPVMDGLEATRRIRSLPPQSTGPFVPIIAMTAHAMRGDRERC
                     LEAGMNDYISKPVTPDVLADVLERWLGTWEGRHPVPEPDVSPFALPDAKGAFEETNRE
                     PDAEALIWDRNVLLERLLGDEEMVREIVHGFLEDIPCQMRALEEAIRACDCVAVRERA
                     HAIKGAAASIQAEALRAASFDVEKAGKSEEADSMPSLFAEMEHCFDLLKQKMNSVEVV
                     RREDTDS"
     gene            5208..>5493
                     /locus_tag="P5201_02080"
     CDS             5208..>5493
                     /locus_tag="P5201_02080"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HRV97349.1"
                     /translation="MRILIAEDDLTSRKILAGVLRKIGHEVVETVDGLAAWAELQKTD
                     GPKLAILDWMMPEMEGLDVVREVRALDIEKPPYIIMLTTRGEKADIIAGLD"
BASE COUNT         1224 a         1401 c         1599 g         1269 t
ORIGIN      
        1 cccgagatgc aacttgcgct ttccaacgca tactacgatt cagcggcgtg gccttggctc
       61 tatgagccct ccttgcttcg cgcaatggaa gccgccggag tactccgtaa actgttctac
      121 ggctccgatt tcccgatcct cgggtatcct aaatacgaga aaatgattgc agcgtccggt
      181 gttgaaagag gagaagttga aaaatttcta catcacaatg cgttcgattt tatggcgaaa
      241 aggtgttgac agaaaccctc atcccttata tactatccct cgcgttgctg gaacggacgc
      301 gggcggatag ttcagcggta gaacacctgc cttacacgca gggggtcaca ggttcgaacc
      361 ctgtttcgcc caccaaggct tcaatggatg tactatgcgg ggtcgtagct cagtcggtta
      421 gagcgtcggc ctgtcacgcc gaaggtcgcg agttcgagtc tcgtcggccc cgccatttac
      481 aatccaattg aggcgggata gctcagatgg tagagcagcg gactgaaaat ccgcgtgtcc
      541 ccagttcgat tctgggtccc gccaccactt ttgaacttag aaaaaggacg cggaagtagc
      601 tcaggggtag agcacaacct tgccaaggtt ggggtcgcgg gttcaaatcc cgtcttccgc
      661 tccattttta aaggatatgc ggcggcatag ccaagtggta aggcagagga ctgcaaatcc
      721 tttatcccca gttcgaatct gggtgccgcc tccaaatata cacattcctc ggtagctcaa
      781 tcggcagagc gggtggctgt taaccactag gttcgaggtt cgagtcctcg ccgaggagcc
      841 agaaagtttc caggccattc tggatttttc cggaaggcct tttttatttg agtcttgccg
      901 cgcgcgtact tccctcgaag agagccctta ccgaagtctt ttttcggcgc gctggatgat
      961 cggaatatag aatagaagaa aagaagaaga gaggtggata tatgtttaaa aagttagtgt
     1021 acatggctgg aaaaccattc tggcttctgc ccgagggcag tctgctcgac gtcgaacagc
     1081 gtaacggccg tatcgtcgat ccggttgttt tccgcaagaa gaacgacgac agaatcaacg
     1141 agaacactca tctgagtatg aattagtcct tcccaacggg aagtgcacct aatacagatc
     1201 ggctgtacag gctccggtaa tccggggcct gttttttttg gtctctttcg ggaagtagcc
     1261 ggccggtcat ttcgcttccg caggttcgcc cggcacatct tcgacgctcg ttacgaaaca
     1321 accttctctt caaattcatg atacaatata aaagtaatac atctgctttt ttctttgcgg
     1381 ctccgtattt tgaatttggt tctcacgagg tcgagctatg gaccagatgc agtcggggaa
     1441 gtcccgtaac aatgacagga cggtacaaag tgtctccgcg cttttgtgtt ccataagcgc
     1501 ggcgatgtcg ttatttaatg ccatcaacaa gacgtacttc aacgccgtgc ttgtattcct
     1561 ctgtcttttg ctgctgacct ttttttatgc gtactatcgg cggacgaaaa atttttcagc
     1621 ctggtcaact ctgtttttat tggtctccgg ctccatggtc gtctatctct tttaccaggg
     1681 agggacgaaa ggcgccggtc ttctttgggc ctatatcttc ccgttcctcg cgtttcaatt
     1741 caaagggtac aaggcaggct tgcgctttgt cgctgttctc tacgcagtgc tggccggcgt
     1801 gttcgcgtcg gctttgactg gacatgcccc cctccacttt tcccctcttt tcatgatcgt
     1861 ctatttctcc gttcatataa cgatctccgg ttttcttttc ttttacgaaa agcggcgaac
     1921 aatcaccgag cggatcattt tcgagagcag ggagaagtat aaggcgctct tcgacaattt
     1981 ttcggtgggg gtggccatga tttctccgga gtttgagctc ctggagctga acggtatcgc
     2041 gagaaaatgg tttgatcgct ctccggaaaa gcagcattct ttttgctacg aatttctgga
     2101 ttcggagcaa aaactcggcg tctgcgaaga ctgccctctg cactcggtaa tggagtcgaa
     2161 acatacggcg acgcagtaca ggaaaaagat gacagtagac ggcatccgta atttcaagat
     2221 agtctgcagc ccgatgttcg acaaggaagg gaatgtttcc gcggtcatcg aaactctcga
     2281 ggacgttacc gaaattcaac gggcgcgtga attccttgag gagagcgaga acaggttccg
     2341 cactcttttt gaacaggtcc catttatttc cgtacagggg tacgacaaag agagaaacgt
     2401 gatcttctgg aacagcgcga gcgagctcct ctacgggtac cctgcgaaag aggctcttgg
     2461 aaaacgactg gaggatctca ttatacccgc cgaaaaaata caggagcatg tcgaagccat
     2521 ctcggcatgg atacaaggag gcccgcctct tcctccctcc gaggtcatgc tgaagcgggc
     2581 tgacggcagc tccgtgcatg tcttctccag tcacgtgctg atccggaatg tacgaggaga
     2641 gaaggagttc tacgctattg atattgacat aaccgaacgg aagcgtcttg aggagcattt
     2701 gagttcgaat gaggagaagt accgtcttct ggttgaaacc gcccaggagg ccatctgtgt
     2761 cgcccaggac gaacgactgg tctacttcaa ccccacggtg agcgatatgc tcgggtattc
     2821 cgacagcgag ctttcttcga tgcactttct tgaaatggtc tatgaagacg acagggagct
     2881 gctgctgcaa aactatcgaa agcgccttgc agggagcggc gttgagcaga gctacgaaat
     2941 ccgtctgttg cgaaaggacg gcacagtggc gtgggtggag ctgaaagcga ccctcatcga
     3001 gtggaacggc agcccggcca cgcttgtctt cctcaacgac atcacgacgc agaaagccgc
     3061 tctgagagag cttatggaga cgaaccgcag gctggagctt gccatcgagc gaacaaggga
     3121 gttcgccaca aaggccgatg cggcgaatcg ggcgaaaagc gattttcttg ccaacatgag
     3181 ccacgaaata cgcacgccga tgaacggcgt tctcgggatg gcgggtttgt tgctggatac
     3241 cgagctgaac cgagagcagc ggcggtgtgc agaggctgtg cgcgcaagcg ccgaatcgct
     3301 tctcggcgtg ctcaacgata tccttgattt ctccaagatt gaggccggca agcttgatct
     3361 tgaacgcctt cgattcgatc tttccgttat gcttgatgat tttgccgccg gtatggccgt
     3421 tcgtgcacac caaaaggggc tcgagttcgt ctgtgcgccg gacccggaag ttccatcccg
     3481 cctcttgggc gatccgggac gtctccgcca gattcttacg aacctcgttg gcaacgccat
     3541 caagtttaca tccaaagggg aggttgtcgt gcgcgtttcc ctggaggagg agagcgaaca
     3601 gagcgcgctt ctccggtttt ccgtgaggga tactggaatc ggcgtgcccg atgaaataaa
     3661 ggagagcgtc tttgaaaagt ttatccaggg agactccacg acaacccgcc gttacggggg
     3721 cacagggctc gggctttcca tctcgaaaca gctggttgag atgatgggag gaaggatcgg
     3781 cgtggaaagc cccccgaagc tttctgctga gaacggtctt tcccattgga gcgaggcggg
     3841 gcccgggtcc gacttctttt ttacggtccg atgcgaaaaa cagccttctg aagtggagtc
     3901 cggtattgtc gtacagggag agctgagcgg agttcgggtg ctgatcgttg acgacaacgc
     3961 aaccaatcgg gagatcgtcc gcgcgcatct cgtctcccgc ggtatgcgtc ctttcgaggc
     4021 gcaggatgga gcctccgccc tccggttgct tgaggaggct gttgagcagg gcgatccggc
     4081 cgctctcgcc ctcgtcgata tgaacatgcc ggaaatggac ggcgaaacgc tgggaagaaa
     4141 aatccgtgag gatgggcgtt tctcacagac gcggctggtt atgctcacct cgcttgcgga
     4201 cagaggagac gccaaacgtt ttgcggaggc cggattttcc gcctaccttc ccaaacctgt
     4261 ccgtgcccgc gaactccttg gagtacttgc gttggtgcat gccaggggag aagatgtctc
     4321 tccggacttt ttcgccacac gtcactcgct gaacgaactt tcatcggcgc acccccggcc
     4381 ttttgctggg aaggggtacc gtatcctcgt cgtggaggat aattccacca accggcaggt
     4441 tgcgctcggg atactcggaa agttcggcgt agaggcctgt tccgtcgaga acggagaaaa
     4501 ggccgtcgag gaggtcggca gaacagcgta cgatctgctc cttatggatg tgcagatgcc
     4561 cgtgatggac ggacttgagg ctacccggcg gattcgttcc cttcctccgc agagcaccgg
     4621 tcctttcgtg ccgatcatcg cgatgacagc gcatgcaatg cgtggagaca gagaacgatg
     4681 tcttgaagcc gggatgaacg actatatctc caagccggtg acgcccgacg tacttgcgga
     4741 tgtccttgaa cgctggcttg gtacatggga agggcgccat cccgttccag agccggatgt
     4801 atcgccgttc gcgctccccg atgcgaaggg cgccttcgaa gaaaccaaca gggaacctga
     4861 cgcggaggct ctgatctggg acagaaacgt cctccttgag cgcttgctgg gggacgaaga
     4921 gatggttcgg gagatagtgc acggctttct tgaagatatt ccgtgccaga tgagagcgct
     4981 tgaagaggcg ataagggcct gcgattgcgt tgcagtgagg gagcgggcgc atgctataaa
     5041 gggagcggcg gcgagtattc aggccgaagc tctgcgggct gcgtcgttcg atgtggaaaa
     5101 ggccgggaag tcggaagagg cggattcgat gccttctctc ttcgcggaga tggagcattg
     5161 tttcgatctc ttgaagcaaa agatgaactc cgtggaggta gtgcgccgtg aggatactga
     5221 tagctgaaga cgatctgaca tctcgcaaaa ttctcgcagg agtgctccgt aagatcggcc
     5281 acgaggtcgt ggagacggtg gatggtttag ccgcatgggc ggagctgcaa aaaacagacg
     5341 gcccgaagct ggcgatcctc gactggatga tgccggagat ggaggggctg gatgtcgtac
     5401 gagaggtgcg cgccctggat atcgaaaagc cgccgtatat catcatgctg accaccaggg
     5461 gcgagaaggc ggacattatt gccggcctgg acg
//
LOCUS       DAOTIL010000059         5473 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminobacteriaceae bacterium isolate AMR_MDS_3026
            NODE_17682_length_5473_cov_3.202842, whole genome shotgun sequence.
ACCESSION   DAOTIL010000059 DAOTIL010000000
VERSION     DAOTIL010000059.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868050
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminobacteriaceae bacterium (wastewater metagenome)
  ORGANISM  Aminobacteriaceae bacterium
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae.
REFERENCE   1  (bases 1 to 5473)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5473)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:19:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,224
            CDSs (total)                      :: 3,198
            Genes (coding)                    :: 3,184
            CDSs (with protein)               :: 3,184
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5473
                     /organism="Aminobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17682_length_5473_cov_3.202842"
                     /isolate="AMR_MDS_3026"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:3034609"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1059)
                     /locus_tag="P5201_02085"
     CDS             complement(<1..1059)
                     /locus_tag="P5201_02085"
                     /inference="COORDINATES: protein motif:HMM:NF019843.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRV97350.1"
                     /translation="MKHSEHRRKGFSEIGIITTILLFLVPGGKFFVTVPTAMEEFIEG
                     DKMEEMEEVFREQSIELDEYVQNAALNPPADTAAESTGNLLKGLPLGLFDSLVLTTEE
                     KALMGDIEAQNELGWMYDTGEGMPENVEEAVKWYRKAAEQGNSVAQANLAWMYEKGRG
                     VPLDLQLAVEWYSKAAEQGLPRAQNNLGVLYEYGTGVPKDEKKAVELYTKAAEQGYII
                     AQTNLGWMYENGRGVRQNDRKAVEWYRTAAEQGDSRAQTNLGWMYENGRGLRRDEKKA
                     VEWYRKAAEQGNLYAQTNLGWMYESGSGVDKNETEAAEWYRRAAEQGHARAQTNLAWL
                     YQNGLGVPQSDEETVEWYT"
     gene            complement(1154..2845)
                     /locus_tag="P5201_02090"
     CDS             complement(1154..2845)
                     /locus_tag="P5201_02090"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRV97351.1"
                     /translation="MKKCASWYNNATMQARYAQRRTLSFQNPPSCETRSEGASFHSHE
                     YGFRRSGPSIREGGIPVIRKGCFVFFTLLWGLWCVAPLPALAGVHAVDDVEYSTPHGW
                     VQQESPGAKAYHVLMYQGHPVGELFLMKESLFEQKSPTTVLQEGIRKNAAGFAGYQAI
                     GMRNISLSGTEAVMHDFLYYPPGSPVQFAGRVVVLVVDKSAYTFFFNTTINTAAMLEG
                     PFSEVVSSVRVVRKPVAPPRKVQEGVFTIELAPGWRDANDLKEEGVHCYDYYDEAEKD
                     TVLVSLLAFGRSKEDESTALSAILQKKNPLEAVLNAMIDMHKRRGKYVPLQTKTRSVA
                     GCDAIVHDFSIQKPDSNFVYRLCIIAVKQQADTPSRVFAPAMYDFTFSSFEMDRFNKG
                     KAAVDALLDSMKLMQPLPALSPPPPFADLQDERIFRSPTEHFTLTLPEGAKQDAMEQL
                     DPVDVLGESFTYTIEGKKDTIILLSIFYSALDGEFAHEELLDELEAKESGQATWEIQG
                     RNVPVGLYSARGGKALVTALFADDDLLLAVLLPKKEYVAAQGWIKELINGVRFEK"
     gene            complement(2928..4127)
                     /gene="trpB"
                     /locus_tag="P5201_02095"
     CDS             complement(2928..4127)
                     /gene="trpB"
                     /locus_tag="P5201_02095"
                     /EC_number="4.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005660920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004834 - tryptophan synthase activity
                     [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan synthase subunit beta"
                     /protein_id="HRV97352.1"
                     /translation="MTQTHNGQTQRGYYGEYGGRFVPEGIAELLAVLERAFDDAVADG
                     AFQKELDTLLVEYVGRPSAITECVNLSEECGGGRLFLKREDLNHTGAHKINNALGQAL
                     LAKRMGKKRLIAETGAGMHGTASATAAALMGMECTVYMGAVDVERQAPNVSRMKALGA
                     KVVPVHDGQATLKEAVDSALMAWMNDLEAFYLLGSVVGPSPYPEVVRYFQSVIGREAR
                     TQMLEREGRLPDEVAACVGGGSNAIGLFSGFLNDPEVAVTGVEPAGKGLQFGLGAHAA
                     TLSAGVPGKVHGFNTYVLFDEKGEPAPVHSVAAGLDYPGVGPEHSYLKDTGRAKYVSV
                     TDAEALDAFSRLCRKEGIIPAVESSHALAYALQRLPSMRKDQILLVNLSGRGDKDLET
                     VLPMLGL"
     gene            4471..4938
                     /locus_tag="P5201_02100"
     CDS             4471..4938
                     /locus_tag="P5201_02100"
                     /EC_number="2.1.1.63"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013047882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003908 -
                     methylated-DNA-[protein]-cysteine S-methyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylated-DNA--[protein]-cysteine
                     S-methyltransferase"
                     /protein_id="HRV97353.1"
                     /translation="MLCVRKTSIGRIAIGGEEEYVTHLYLPRSTELIERFDAKETPVI
                     REAFRQLELYLEGKLREFSLPLRAEGTPFMKRVWSELVKVPYGATTSYKELAEASGNP
                     KAVRAVGTANAKNPIAIFIPCHRVINTGGKLGGYGGGLDLKKTLLELEARNRP"
     gene            4951..>5473
                     /locus_tag="P5201_02105"
     CDS             4951..>5473
                     /locus_tag="P5201_02105"
                     /inference="COORDINATES: protein motif:HMM:NF020476.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1848 family protein"
                     /protein_id="HRV97354.1"
                     /translation="MRIVSVSRRTDIPAFYSPWLFRRLKDGYVLVRNPRNAKQTRFVS
                     LKPDDVHCFVFWTKDARPMFGRLDELEAHESAFYFMWTVTPYGPEIEPGFPRKEEIVS
                     AFRQLSGRIGPRRTIWRYDPVVLDDSFPVRKHLEAFARMCASLDGATERCIVSFVDMY
                     RKLRGKGIREVAEE"
BASE COUNT         1017 a         1692 c         1580 g         1184 t
ORIGIN      
        1 ggtataccac tccaccgtct cctcgtcgct ctggggtacg ccgagcccgt tctgatagag
       61 ccatgcaaga ttggtctgcg cgcgcgcatg tccctgttcc gcagctcttc ggtaccattc
      121 tgcggcttcc gtttcgttct tgtcgacgcc gctgccgctc tcgtacatcc atccgaggtt
      181 tgtctgcgcg tacagattgc cctgctcagc ggctttgcga taccattcga cggctttttt
      241 ctcgtcgcgg cggagccccc tgccattttc gtacatccat ccgaggttcg tctgcgcgcg
      301 cgaatccccc tgttcggcag ccgtgcggta ccattcgacc gctttgcggt cgttctgacg
      361 aactcctctg ccgttttcgt acatccatcc gaggtttgtc tgcgcgatta tgtacccctg
      421 ctccgccgcc ttggtgtaga gttcgaccgc cttcttctcg tccttgggaa ctcctgttcc
      481 gtattcatac agtacgccaa ggttgttctg agcgcggggg agcccctgtt cagcggcctt
      541 tgagtaccat tcgaccgcga gctgcaggtc cagcgggacg cctctgcctt tttcgtacat
      601 ccaggccaga ttcgcctggg cgaccgagtt gccctgttca gcggcctttc ggtaccactt
      661 gaccgcttct tccacgtttt ccggcatccc ttcgccggtg tcgtacatcc agccgagttc
      721 attctgcgct tcaatatccc ccataagcgc cttctcctcc gtggtcaaca ccagagagtc
      781 aaaaagcccc aaaggaagcc ccttcaagag attgcccgtt gattccgcgg cagtatccgc
      841 cggagggttc aaagcggcat tttggacata ttcgtccagt tcaatggact gctcccggaa
      901 gacctcctcc atctcttcca ttttgtctcc ttcgataaat tcctccattg cagtgggaac
      961 agtgacaaag aatttacctc ccgggacaag aaacaaaaga atggtcgtga ttattccgat
     1021 ttcagaaaac cctttccgcc ggtgttcgct gtgtttcatg tctcactcct tctttctgtc
     1081 gggggtttta ggaaagacct tatcggtttt cccgtaatgc cgttcttttt caaagagagc
     1141 gcttccgcag aggctacttt tcaaagcgga cgccgttaat cagctctttg atccacccct
     1201 gcgcagccac gtactctttc ttcggcagca ggacggcgag gaggaggtcg tcgtctgcga
     1261 agagcgccgt gaccagagcc ttcccgccgc gcgctgaata cagtccgacg gggacgtttc
     1321 ttccctggat ttcccaggtc gcctgcccgc tctcctttgc ttccagctca tcgaggagtt
     1381 cctcgtgcgc aaactccccg tccagcgcac tgtagaagat ggacagaagt atgatcgtgt
     1441 ccttcttccc ttcaatcgtg taggtgaacg actctccgag gacgtcgacc gggtcgagct
     1501 gttccatggc gtcctgtttt gctccttcgg ggagcgtcag tgtgaagtgt tcggtaggag
     1561 agcggaagat gcgttcgtcc tgcaagtcgg cgaaaggcgg aggaggagac agggcaggga
     1621 gaggttgcat aagcttcatc gagtcgagca gcgcgtcgac cgccgccttc cctttgttga
     1681 aacggtccat ttcaaacgag gaaaaggtaa aatcgtacat tgcgggagcg aatacccgcg
     1741 aaggcgtatc cgcctgctgt ttgaccgcga tgatgcacag acggtagaca aaattcgaat
     1801 ccggcttctg gatggagaaa tcgtggacga tcgcgtcgca gcctgcgaca gaacgggtct
     1861 tcgtctgcag agggacgtat ttcccccttc gtttgtgcat gtcgatcatg gcgttcaaaa
     1921 cggcttccag cggattcttc ttctgcaaaa tcgccgaaag agcggtagac tcatcctcct
     1981 tgctgcgccc gaaggccagt aacgaaacca gtactgtatc cttttcggcc tcatcgtaat
     2041 agtcgtagca gtgaaccccc tcctccttca ggtcgtttgc gtcgcgccac cccggggcga
     2101 gttcaatggt aaaaacgcct tcttggactt ttcgcggcgg cgcgaccggt ttgcggacaa
     2161 cccggacgga ggagacgact tcgctgaacg gtccttcgag catggcggcg gtattgatcg
     2221 tcgtattgaa gaaaaaggtg taggcggatt tgtccacaac gagcacgacg acccttcccg
     2281 cgaactgcac ggggctcccc ggcgggtagt agaggaagtc gtgcatcaca gcctcggttc
     2341 cggagagcga gatgttcctc atgccgatcg cctggtaccc cgcaaatccc gcggcgttct
     2401 tccggatgcc ttcctggagg actgtcgtag gacttttctg ttcgaagagg gactctttca
     2461 tcaggaagag ctcgccgacc ggatgcccct ggtacatcag gacatggtat gccttcgctc
     2521 ccggagattc ctgttgcacc catccgtgcg gcgtggagta ttcaacgtca tccacggcat
     2581 gcacccccgc gagcgccgga agcggcgcaa cgcaccacag accccagagt aacgtgaaga
     2641 atacgaaaca ccctttgcga atcaccggta ttcccccttc ccgtatcgac ggtccgctgc
     2701 gcctgaagcc gtactcgtga gagtggaacg acgccccctc cgagcgcgtt tcacaggagg
     2761 gaggattctg aaagctcaaa gtgcggcgtt gcgcatatcg ggcctgcatc gtcgcattat
     2821 tgtaccacga tgcgcacttt ttcatcaggt tgtctttggc gggaagagcg tagttttttg
     2881 agagaagtga gcgctgccgg agggactctc cggcagcgtt cgcatgtcta cagcccgagc
     2941 atcggcagca cggtctccag atctttgtct ccgcgtccgg agaggttgac cagcagtatc
     3001 tgatcctttc gcatcgaggg gaggcgttgc agcgcgtacg ccagcgcgtg cgagctttcc
     3061 accgcgggga tgatcccttc cttcctgcag agacggctga atgcgtccag cgcttcggcg
     3121 tccgtgacgg agacgtactt cgcccgcccc gtgtccttca ggtaggagtg ctcggggccg
     3181 acgcccggat agtcgaggcc cgccgccacg gagtgcacgg gagcgggctc tcccttctcg
     3241 tcgaagagga cgtacgtgtt gaagccgtgg accttgcccg gaacgccagc ggagagggtg
     3301 gccgcgtgcg cgcccaaacc gaactgcaac cccttccccg cgggttccac gcccgtgacg
     3361 gcgacctcgg gatcgttcag gaatccggag aagaggccga tggcgttgct tccgccgccg
     3421 acgcatgcgg ccacctcgtc gggaagccgg ccttcccgtt cgagcatctg tgtccgagcc
     3481 tcccgtccga tgacgctctg gaagtagcgc acgacttccg gatacggcga cggtccgacg
     3541 acggagccga ggagatagaa agcctccagg tcgttcatcc acgccatgag cgcgctgtcc
     3601 acggcctcct tgagcgtcgc ctggccgtcg tgtaccggga cgactttcgc gcccagcgcc
     3661 ttcatcctgg agacgttcgg cgcctgacgc tcgacgtcga cagcccccat gtacaccgtg
     3721 cactccatcc ccatcagggc cgccgccgtc gcgctcgccg tgccgtgcat tcccgcgccc
     3781 gtctcggcga tgagacgctt ctttcccata cgcttcgcca acagcgcctg tccgagcgcg
     3841 ttgttgatct tgtgcgcgcc ggtgtggttc agatcctccc gcttcaggaa aaggcgtccc
     3901 ccgccgcact cctccgagag gttgacacac tccgtgatcg ccgacggcct gcctacgtac
     3961 tcgacgagca gcgtgtccag ctctttttga aacgcgccgt ccgccaccgc gtcgtcgaag
     4021 gccctctcca gcactgccag cagctccgcg atcccctccg gcacgaaccg tccgccgtac
     4081 tccccgtaat acccccgttg tgtctgtccg ttgtgtgtct gcgtcatttc gatctcctcc
     4141 ttcgaagttg cgaagatgct cttcgctgaa tacaaaaagg gccgtctcaa gcgacggccc
     4201 tttgaaacta cggatacaaa aagggccgcg ggaaacgttt gctcccgcgg ccacctgaaa
     4261 acaggtcaaa ggcggcggga ccctagggcc gccaccacca catgttcatc gtcgtaaaac
     4321 tccggtcgag tgtgtgcgct gtctttttca tgcagggaca atagcacggc gaacccagtt
     4381 ttgtcaatgc gcatttttca aatcgctttt ttttcgtttt tgagagaaaa tagccgggcg
     4441 gaaagagatc agttggagag gaggaccaca atgctgtgcg ttcggaagac atcgatagga
     4501 agaatcgcca tcggcgggga agaggaatac gttacgcatc tctatctgcc gaggagtacg
     4561 gagttgattg aacgcttcga tgcgaaggag acgcccgtga tccgggaggc ctttcgccag
     4621 ttggagctgt acctcgaagg gaaactcagg gagttctcgc tgcccctccg ggccgagggg
     4681 acgcccttca tgaagagggt ctggagcgag ctggtgaaag ttccctacgg ggcgaccacg
     4741 agctacaagg agctcgccga ggcctcaggg aatccgaagg cggtccgcgc cgtgggaacg
     4801 gcgaacgcga agaaccccat cgccatcttc atcccctgcc accgcgtcat caacaccggc
     4861 ggcaagctcg gcggatacgg cggggggctc gacctgaaga aaacgctcct cgagctggaa
     4921 gcccgcaaca gaccgtagga tgtcgttcgt gtgcgcatag tcagcgtgag cagacggacc
     4981 gacatcccgg cattctactc cccgtggctg ttccgccgtt tgaaggatgg gtatgtcctc
     5041 gtccgcaatc cgcggaacgc gaagcagacg cgcttcgtct cgctgaagcc ggatgatgtg
     5101 cactgcttcg tcttctggac caaggacgcc cggccgatgt tcggacgcct cgacgaactg
     5161 gaggcgcacg agagcgcgtt ctactttatg tggacggtca cgccgtacgg cccggagatc
     5221 gaacccggct ttccccggaa agaggagatc gtgtccgcct tccggcagct gagcggacga
     5281 atcggcccgc ggcggacgat ctggaggtac gaccccgttg tcctcgacga ctcgttcccc
     5341 gtccggaagc acctggaagc gttcgcccgc atgtgcgcgt cgctcgatgg ggcgacggag
     5401 cggtgtatcg tcagcttcgt cgacatgtac cggaagctgc gcgggaaggg gatacgcgag
     5461 gtcgccgagg aag
//
LOCUS       DAOTIL010000060         5447 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminobacteriaceae bacterium isolate AMR_MDS_3026
            NODE_17782_length_5447_cov_3.196402, whole genome shotgun sequence.
ACCESSION   DAOTIL010000060 DAOTIL010000000
VERSION     DAOTIL010000060.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868050
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminobacteriaceae bacterium (wastewater metagenome)
  ORGANISM  Aminobacteriaceae bacterium
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae.
REFERENCE   1  (bases 1 to 5447)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5447)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:19:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,224
            CDSs (total)                      :: 3,198
            Genes (coding)                    :: 3,184
            CDSs (with protein)               :: 3,184
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5447
                     /organism="Aminobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17782_length_5447_cov_3.196402"
                     /isolate="AMR_MDS_3026"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:3034609"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(17..547)
                     /locus_tag="P5201_02110"
     CDS             complement(17..547)
                     /locus_tag="P5201_02110"
                     /inference="COORDINATES: protein motif:HMM:NF016200.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter small permease subunit"
                     /protein_id="HRV97355.1"
                     /translation="MIKFINFTDTLNDWVGKIFGFLVYPTMLVLVYEVVMRYGFNRPT
                     IWAHETSCMLYGAHFILGGAYALRWGGFVNVEAFYILFSPRVRAIIDLFTWTLFYAFV
                     GTLLWFSLPWAWESLQMMEYSNSTWGPYVWQLKLTIPAAAFLVLLQGMTKTIKDLHLA
                     LTGRELIPSAEAPVEN"
     gene            complement(656..1714)
                     /locus_tag="P5201_02115"
     CDS             complement(656..1714)
                     /locus_tag="P5201_02115"
                     /inference="COORDINATES: protein motif:HMM:NF015445.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter substrate-binding protein"
                     /protein_id="HRV97356.1"
                     /translation="MKHGKLVALSVAVAVILGIFIIGVPSNAVAAEFNWKFQSHHTPG
                     ALSTTYVIPPFIDMVREKSGGRLNITLHYAGELVDYLEVFPSLQANMIQISNTSTLFW
                     RGSLNVGWLQAGNLPPFVCRSLEDFEELYYKRGIDRLIGEGLAERDIHFLGSHSVGNT
                     YFWSKKPINTLDDLKGFKVRFFGAMNDTMEHFGASPVMLPHPETYMAIAMGTLDGSGT
                     AWWLYRDLKLYEVCPYFIGPAWQVPQGMELWVSKKAWDALPDDLKKVVEEATVEFNKT
                     FAKVCDEQEKEMFAKSFKEWGTTYIEWGKDDIDRITKEFSLPFLNKIEQEIGSKDPRV
                     AEGIQIIKQFMKDRGYIN"
     gene            complement(2049..2552)
                     /locus_tag="P5201_02120"
     CDS             complement(2049..2552)
                     /locus_tag="P5201_02120"
                     /inference="COORDINATES: protein motif:HMM:NF013077.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HRV97357.1"
                     /translation="MQDIILKRRSIRKYTEEPVSEEAIRLLLEAGMSAPSAGDERPWH
                     FIAITDKAVKEQIPKHHPYASMTPQAPLVIAVCADLKQQRYPGYWPQDCAAATQNILL
                     SAETQGLGAVWLGVQPDTSRSKGLGALFGIPEGIEIFSLIAVGHPAEKKESPSRYDEK
                     RVHRDRW"
     gene            3035..4168
                     /locus_tag="P5201_02125"
     CDS             3035..4168
                     /locus_tag="P5201_02125"
                     /inference="COORDINATES: protein motif:HMM:NF014516.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HRV97358.1"
                     /translation="MRGAIKKISISGFKSIRVLKEFELKNLNIFIGANGTGKSNLVQV
                     FQLLMAMTRKNLQKFILENGGADNFLHNGPKNTSAITIEFEFESHSDFARGSNSYRFE
                     LTPTVNETFLIDEERKYVTSNWRSYGSPSPESRLHDERDEKSADDSRNGIGHFVYESI
                     AHWMVYHFHDTSATAPMRRSEIVEDNHILRSNGGNIAPFLLRLKNTDFRRSFYQEIVD
                     AVRLVIPFFDDFLLDVAKYGEAEKVKLTWQQKGSDFPMQPYHLSDGSIRFICLATALL
                     QPSPPSAIVIDEPELGLHPEAIRILGELIEDCAKRTQIIVATQSPLLLDQFSIEDIVV
                     VNRREGQSTFDRLQRADFNEWLEEYSVGELWTKNVIRGGTTHE"
     gene            4161..4853
                     /locus_tag="P5201_02130"
     CDS             4161..4853
                     /locus_tag="P5201_02130"
                     /inference="COORDINATES: protein motif:HMM:NF025468.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4276 family protein"
                     /protein_id="HRV97359.1"
                     /translation="MSSCTEVVVLVEGKTEQRFVSELLAPYMADRGVYLTPIILDKPG
                     EKGGDVKFSRAGKDIGRFLKQRGDTLITLFIDFYGIRADWPGYAESKRQTDHAAKAAI
                     MNQATAEQIQLLFPEQNRERRFIPYVSMYETEGLYFSDPACLAGFLGVDQRRVDAILT
                     ECGEPEKINDHSTTAPSKRLAALSPRFKKTTTGISIAREIGIPKMRAACPLFDAWLAK
                     LESLAVCDDSES"
     gene            5087..>5447
                     /locus_tag="P5201_02135"
     CDS             5087..>5447
                     /locus_tag="P5201_02135"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRV97360.1"
                     /translation="MEKLSKWFSERPQWLQMAARKLLKQSELTEKDVSELATLCQQEV
                     VEKLAKKTCSFPPFVFSQGVAGTLRLCSISDVEGVNALAPKKPLEFGKGNITIVYGNN
                     GSGKSGYVRLLKHVCGAR"
BASE COUNT         1285 a         1411 c         1463 g         1288 t
ORIGIN      
        1 tgtccataat agcacctcag ttctcaaccg gagcttcggc cgagggaata agttcgcggc
       61 cggtcagcgc caggtggaga tccttgatgg tcttggtcat cccctggagc agcacaagaa
      121 acgccgctgc gggtatggtc agcttgagct gccagacgta cgggccccac gtgctgttcg
      181 agtactccat catctgcaaa ctctcccacg cccacggcaa actgaaccag agcaaagttc
      241 cgacgaacgc gtaaaaaagc gtccacgtaa agagatcgat gatagcgcgc acccgcggtg
      301 agaagagaat gtagaacgcc tctacgttga cgaaccctcc ccagcggagg gcgtacgctc
      361 ctccaagaat aaaatgcgcc ccgtacagca tacacgaagt ttcgtgcgcc catatcgtag
      421 ggcgattgaa tccgtagcgc ataaccacct cgtacacgag tacgagcatt gtcgggtaca
      481 cgagaaagcc gaaaatcttc cccacccagt cgttcagtgt gtcggtaaaa ttaataaact
      541 tgatcaaaaa gcccaatcgc ctcacctcgc cgattctgaa aaaattgaaa gcgcgccccc
      601 tcctccggag agagaagggg ggcgcttcgt ggatgcgtcg ggtggacgct ccctgttagt
      661 tgatgtaccc gcgatccttc atgaactgct tgatgatctg aataccttcg gcaaccctcg
      721 gatccttcga gccgatttcc tgttcgatct tgttcaggaa ggggagagag aattctttcg
      781 ttatcctgtc gatgtcatcc ttgccccact cgatgtaggt cgtcccccac tccttgaagg
      841 acttcgcgaa catctctttt tcctgctcgt cgcacacttt ggcgaacgtc ttgttgaatt
      901 ctactgtagc ttcctcgacg accttcttca ggtcgtcggg cagggcgtcc cacgccttct
      961 tggacaccca cagttccata ccctgaggca cctgccatgc ggggccgatg aagtacgggc
     1021 acacttcgta gagcttcaga tcgcgataga gccaccaggc ggtgccgctg ccgtcgagag
     1081 tgcccatcgc gatggccatg taggtctcgg gatgcggcag catgaccggc gacgcgccga
     1141 agtgctccat ggtgtcgttc atcgcgccga agaaacggac cttgaatccc ttgagatcgt
     1201 ccagcgtatt gatgggcttt ttactccaga agtaggtgtt gcccacgctg tggcttccca
     1261 ggaagtgaat gtcccgctcg gccagtcctt ctccgatgag tctgtcgatg cctctcttgt
     1321 agtacagctc ctcgaagtcc tcgaggctcc ggcagacgaa ggggggcaga ttgcccgcct
     1381 ggagccaacc gacgttcagc gagcctctcc agaagagcgt gctggtgttg gagatctgga
     1441 tcatgtttgc ctgcaaagag gggaacactt ccaggtagtc tacgagttct cccgcatagt
     1501 gcagggtgat gttcagtctg ccgcccgact tctcgcggac catatcaata aacggcggga
     1561 tgacgtatgt cgtcgacagc gctcccggtg tgtgatggct ctggaacttc cagttgaact
     1621 ccgccgctac tgcgtttgaa ggtacgccga taataaaaat gcccagaata accgctaccg
     1681 ctacgctcaa tgcaaccagt tttccgtgtt tcatcaaaac acctccctct agagtaagtg
     1741 ccgaacccgg agcttctttt ttcatctatc ccctcctttg tcgtcatgac ctgcaactcc
     1801 aaagaactgt gtgcgaaaag gaacactgga tatgaacaaa aagcagtctt ttggactaaa
     1861 agaaagctct gttgttttca gaaaaaaata atcctcagct tgtatgcagt tgatgcaagg
     1921 gttaagggca tcgctatttc tctgttgttt tgccagtgta cttgctcgaa tttgtaaagt
     1981 caagatatac atgaaaagag cactgtgctc gcatgcactg aataccggtg tatgcagaat
     2041 gccagctcct accagcggtc gcgatgcact ctcttttcgt cgtagcgcga ggggctctcc
     2101 tttttctccg ccgggtgacc gacggcgatc agcgaaaaga tttcgatccc ctccggaatg
     2161 ccgaaaagcg cgccgagccc cttcgagcgg gaagtgtccg gttggactcc cagccagacc
     2221 gccccaagtc cctgcgtctc cgccgagagc aggatgttct gcgtcgcggc tgcacagtcc
     2281 tgcggccagt agccggggta gcgctgctgc ttcagatcgg cgcagaccgc gataacgagc
     2341 ggcgcctgag gcgtcatcga ggcgtagggg tggtgcttcg ggatctgctc cttgaccgcc
     2401 ttgtccgtga tcgcgatgaa atgccagggg cgctcgtccc ccgccgaggg agcgctcatc
     2461 cccgcctcca gaagaaggcg gatcgcctcc tcgctcacag gctcctccgt gtacttgcgt
     2521 atgctccgcc gtttcaaaat gatatcctgc atcgggaaaa cctcctatcg ggttgagttt
     2581 tgcacgctgg gaggatgttt ctcctcagaa taaggatatt gcgtttgtaa aataaagaca
     2641 agtatctccg cgttgtgctt cggcaaatat agagaacagg gacaaccgga atatgtttct
     2701 gatcaccatc agcgttgagc ggacaagatt tcactctcgg atccgcgctc gacggtaaag
     2761 tgcataatgc gttataattt cactcaactt cgtcagtctt tgtatttcag tatacattgg
     2821 gatacgcggg taaaaaacga gagagattac gtcggatggt cgcaattctt ttccggacag
     2881 gctatgaatt cagtgttttt tgtcttgata accgggttca cttttcagac cgaccacttg
     2941 ttccgaccga tctgcagtag gaggacgaga actggcaagt ccgtctcatc gaagaaaagt
     3001 tcacggagtg catgtggaac agggagggcg tggcatgaga ggcgcaatca agaaaatcag
     3061 tatcagcggt ttcaagtcga ttcgggtgct gaaagagttc gagctcaaga acctgaatat
     3121 ttttatcggc gcgaacggca cgggcaagag taatctggtt caagtgtttc aactgctcat
     3181 ggcaatgacg cgaaagaacc ttcaaaaatt catcttggag aacggcggtg cggataattt
     3241 tctgcacaac ggacccaaga acacatccgc aattacgata gagtttgaat tcgagtcgca
     3301 cagcgatttc gccagggggt cgaattccta tcgtttcgaa ctgacgccca ccgtgaatga
     3361 aacattcctt atcgatgagg agcgcaagta tgtgacgagc aactggcgtt cctacggtag
     3421 cccatctccc gaaagtcgat tgcatgacga acgcgatgag aaatccgctg atgacagtcg
     3481 gaacggaatc ggccattttg tctacgagtc cattgcccat tggatggtgt atcacttcca
     3541 cgacaccagc gcgacggcgc ccatgcgtcg ctccgagatc gtcgaagaca atcatatttt
     3601 gcggagcaat gggggtaata ttgcgccttt tttgctgcgg ttgaaaaaca cggattttcg
     3661 ccgttcgttc tatcaggaaa ttgtcgatgc cgttcggctt gttatccctt tcttcgacga
     3721 ttttcttctg gatgtggcca agtacggaga agcagaaaag gtgaaactca cctggcagca
     3781 gaaggggtcc gacttcccga tgcaaccgta ccacctttcc gatggctcca tccgcttcat
     3841 ctgtcttgct acggcccttc tccaacctag tcctccgtcc gccattgtta tcgacgagcc
     3901 ggagctgggt cttcaccccg aggcgatccg catccttggc gaactgatag aagactgtgc
     3961 taaacgaacc cagattatcg ttgccacgca gtcgcccctt cttctcgatc agttttccat
     4021 agaggatatc gtcgtggtga accgcaggga ggggcaatcg acttttgatc gtctgcagcg
     4081 cgcggatttc aacgagtggc tggaagaata ttcggtcggt gaactctgga cgaagaatgt
     4141 catccggggg ggcacgaccc atgagtagct gcacggaagt tgtggtgctt gtggagggga
     4201 aaacggaaca acgcttcgtc agtgaattgc ttgccccgta catggcggac cggggcgtat
     4261 atttgacacc gatcattctt gataagcccg gcgaaaaggg aggcgatgtg aagttctccc
     4321 gcgccgggaa agatatcggc agatttctca agcaacgagg ggataccctg ataacgctgt
     4381 ttattgattt ttatgggatt agggcggatt ggcccggata tgcggagtca aaacgacaaa
     4441 cggatcatgc tgcaaaagct gcgatcatga atcaggcaac cgccgaacag atacaactgc
     4501 tcttcccgga acagaaccgg gagaggcgtt ttattcccta tgtttccatg tacgagacgg
     4561 agggtttgta tttcagtgat cctgcgtgtc tggcgggttt cttgggcgtg gaccaacgcc
     4621 gtgttgatgc gattcttaca gagtgcgggg aaccggaaaa gattaacgac catagtacaa
     4681 cggcgccttc aaaaagattg gcggcgcttt ctccgcgctt caagaaaacg acgaccggca
     4741 tatcgattgc acgggaaatc ggcattccga aaatgcgcgc cgcctgcccg ttgttcgatg
     4801 cttggctggc aaaactggaa tcactggcag tgtgtgatga tagcgaatcg taagccggag
     4861 ggccaaagcc gtatgcattc agaaacaagt tgctgcttta ttcgtgcctg atgagcctgt
     4921 tcggcacgaa tctaggggta aaataaatta attgggaaat agtctgcttt gtcagtcaga
     4981 tgcgccaaac gagcttgaga aagtttgttt ttttgctcat aacagacgac taaggcagat
     5041 aaatcgacta actgtttgcc cgattgaggg ggatggtgaa atgtgcatgg aaaaattgtc
     5101 caaatggttt tcagagcgtc cccagtggct ccagatggcg gcaagaaagc tacttaagca
     5161 gtctgaactt actgaaaaag acgtctcgga actcgcaacc ttgtgccaac aggaagtagt
     5221 cgagaagttg gccaaaaaga cttgttcttt tccccctttc gtgttctctc agggcgttgc
     5281 aggcaccttg cgcttgtgtt caatcagtga tgtcgaagga gtaaacgccc ttgctccgaa
     5341 gaaaccactg gagtttggca agggcaatat cacgattgtt tatggcaaca acgggtcagg
     5401 caaatccggt tacgttagac tcctcaagca tgtatgtggt gcccgcg
//
LOCUS       DAOTIL010000061         5445 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminobacteriaceae bacterium isolate AMR_MDS_3026
            NODE_17790_length_5445_cov_2.935065, whole genome shotgun sequence.
ACCESSION   DAOTIL010000061 DAOTIL010000000
VERSION     DAOTIL010000061.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868050
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminobacteriaceae bacterium (wastewater metagenome)
  ORGANISM  Aminobacteriaceae bacterium
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae.
REFERENCE   1  (bases 1 to 5445)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5445)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:19:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,224
            CDSs (total)                      :: 3,198
            Genes (coding)                    :: 3,184
            CDSs (with protein)               :: 3,184
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5445
                     /organism="Aminobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17790_length_5445_cov_2.935065"
                     /isolate="AMR_MDS_3026"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:3034609"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..535)
                     /locus_tag="P5201_02140"
     CDS             complement(<1..535)
                     /locus_tag="P5201_02140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005658700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter large permease subunit"
                     /protein_id="HRV97361.1"
                     /translation="MGLVLAGSFAFFLLLGVPVGLAIGVSGLLIVFLNGFPLEMIPQT
                     MFSGNESFALVAVPFFVLAGDILAKGGISERIIAFAEATLGRFRGGLSIVSTVASMFI
                     AAISGSGAATTAAVGSALLPQLEEKGYDVDFSAALIAASVVIFSTLAGWAFAKLRFRG
                     SKWLLIFVVATMAVPMQL"
     gene            complement(537..977)
                     /locus_tag="P5201_02145"
     CDS             complement(537..977)
                     /locus_tag="P5201_02145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005658701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter small permease"
                     /protein_id="HRV97362.1"
                     /translation="MNRISEILLFAMMLAMIAVTTMQIVCRVFFEALIWSEELTTYLL
                     VAASLLGAAIAFKRGSHIAVTFLVHKLPLPLRKVTEFLVQGIGILFFFVVAYYGGVLM
                     KSEAMQTTPAMGISMTWIYLMYPVVGSIVLLHLVAGLGGILGRK"
     gene            complement(1058..2032)
                     /locus_tag="P5201_02150"
     CDS             complement(1058..2032)
                     /locus_tag="P5201_02150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014162202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter substrate-binding protein"
                     /protein_id="HRV97363.1"
                     /translation="MKRSIAVVSIVLLTLLFVASAAVAAVDIKLAHVVNENDAFHVCA
                     LKFKELVEAGSKGELTVTIYPNAQLGDERNLLESMRMGVVDSAIITGGPIMNFMPKFG
                     VFDLPFLFSTPEHAYKVLDGEIGKMMLNEMEQHGWKGLAYGERGFRNLTNSKQPVKTP
                     EDMKGLKIRLMQNPVYVDSFRALGANAIPMAWTEALTALQQGAIDGQENPLNVIVAFN
                     LFESQKHLAITRHAYAPNVIMMSKKTWEKLAPEMQSLVQDAAQKAAEHNRAYDNEKEA
                     EWLQFLKDKGMEVTETDTAAFREAVKPVYQKYEEQFGKELIEAILADK"
     gene            2346..2729
                     /locus_tag="P5201_02155"
     CDS             2346..2729
                     /locus_tag="P5201_02155"
                     /inference="COORDINATES: protein motif:HMM:NF019498.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HRV97364.1"
                     /translation="MADFVLLAGKISDIRLAEDPSHASGIEKRIVFAPGKFWNDYVAR
                     HFTVKGKNSKTPFHTHDWPHYVFILSGRCNATIDGTVYPLEKGCWAHVPPNVEHFFES
                     TGEEPLDFICIVPPKGDPAGSAHLA"
     gene            complement(2811..3278)
                     /locus_tag="P5201_02160"
     CDS             complement(2811..3278)
                     /locus_tag="P5201_02160"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRV97365.1"
                     /translation="MTYLNYVFFLVFWGWILSVGYIRYILPFIESAFDTLEAMEKSGE
                     LFPRAIAFIAKLAMTGSQMYILGIWSAYCVLRTMIFLHEPGTNGWLYYITAFLVCEGF
                     LGAVAKKEKYRGLLSVFHSAMAMGLFVIFAMNPYFLRSVYPWLPPMMNFSFPH"
     gene            complement(3429..3905)
                     /gene="gpt"
                     /locus_tag="P5201_02165"
     CDS             complement(3429..3905)
                     /gene="gpt"
                     /locus_tag="P5201_02165"
                     /EC_number="2.4.2.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013047656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000310 - xanthine
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine phosphoribosyltransferase"
                     /protein_id="HRV97366.1"
                     /translation="MSSERYHKTYPVSWEQVHRDCKALAWRLVSVCKWERIIGIARGG
                     LIPAAIIARELDVRLVDTVCVSSYVLRTQGEPSVLKEISGDGAGWLIIDDLVDTGKTA
                     QVVRRMLPKAHFAAVYAKPDGRAFVDTYITEVSQDTWILFPWDAETAYAKPIVEQR"
     gene            4080..5165
                     /locus_tag="P5201_02170"
     CDS             4080..5165
                     /locus_tag="P5201_02170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013047655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BMP family ABC transporter substrate-binding
                     protein"
                     /protein_id="HRV97367.1"
                     /translation="MKRFVVLLVFVLSVTLCGAAYAAFPAIPAEKINPGFVYIGPIGD
                     GGWTYMHEQGRLAMEKEFPGVKSTFVESIPEGPDAARVFETMIRNGSKVIFGTTFGYM
                     DFVQEVAKKYPDTIFMHCAGYKDAPNVGTYFGRMYQARYLSGLVAGKMTKKNRIGYVG
                     AHPIPEVIRGINAFTQGVRKANPEAEVQVIWIFSWLDPGKEKEAAKALIDSGVDILAM
                     HADTGATPQAAEEAGIYVIGYNNDMSAYAPTKHLTAPIWDWGMVYKETIGQVIKGTWQ
                     PSHIWYGLKEGMVALAPYGKDVPDLVKALVDVEKAKIVSGEWDVFHGPVKDQSGEVKV
                     PEGAAMTDGEMLSMNWFVEGVKGDIPK"
BASE COUNT         1333 a         1534 c         1404 g         1174 t
ORIGIN      
        1 ccagctgcat cggcacggcc atcgtcgcga cgacgaagat gagaagccac ttgctgcctc
       61 gaaagcgcag tttcgcgaac gcccacccgg cgagtgtcga gaagatgacg acggatgctg
      121 cgatcagggc ggccgagaag tccacatcgt accccttctc ctcaagctgc gggaggagcg
      181 cggagccgac ggccgccgtc gtagccgctc cggagcctga gatggccgcg atgaacatgg
      241 aggccacagt agagacgatg gagagtccgc ctcggaacct tccgagagtc gcctcggcaa
      301 aagcgataat ccgctccgag atgccgccct tcgcgagaat atctcctgca agaacgaaaa
      361 aagggaccgc gacgagcgca aacgattcat tcccggagaa catggtctgc ggaatcatct
      421 caagagggaa tccgttcaaa aagacgataa gcagacccga tacgccgatg gcaagtccca
      481 ccggaacccc caggagtaaa aaaaacgcaa aactgcctgc aagaacgagt cccatattac
      541 ttcctcccga gaatgccgcc gagtccagcg acaagatgca gcagaacgat actccccacg
      601 accgggtaca tcagataaat ccatgtcatg gaaataccca tggctggagt tgtctgcatc
      661 gcctcggact tcataagaac gccgccgtag tacgcgacga cgaagaagaa gagaatccct
      721 atcccctgca caaggaattc cgtcactttt ctcagcggaa gagggagctt gtgtacaagg
      781 aacgttaccg ctatgtgaga gcctcgtttg aaagctatcg cggcgcccag gagcgacgcg
      841 gcgaccagaa gatatgtcgt cagttcctcc gaccagatca gcgcctcgaa gaagaccctg
      901 cagacaatct gcattgtcgt cacggcaatc atcgcgagca tcatcgcgaa gagaagtatt
      961 tcgctgatcc tattcacaaa atcgcttaat cggacaagag gcgagaatac gctcccgcct
     1021 cttgtcccgt tatctctgga attcatccgc tacagcctta cttgtcagcc agaatagcct
     1081 cgataagctc tttaccgaac tgctcttcgt acttttggta cacaggtttg acagcctcgc
     1141 ggaacgcggc cgtgtcggtc tctgtaactt ccattccctt atccttcagg aactggagcc
     1201 actcggcctc tttctcattg tcgtaggctc tgttatgctc cgctgccttc tgcgccgcat
     1261 cctgaacaag ggactgcatc tcgggggcaa gtttttccca cgtctttttg ctcatcataa
     1321 tcacgttcgg cgcgtaggca tggcgggtga tcgcaagatg tttctgggac tcgaaaagat
     1381 tgaaggcaac gatgacgttc agcggattct cctgtccgtc gatagctccc tgctggagtg
     1441 cggtaagcgc ctcggtccac gccataggga tcgcgttcgc gccaagcgct ctgaaggagt
     1501 cgacatagac cgggttctgc atgagccgga tcttcagccc cttcatatcc tcgggggtct
     1561 tgacaggctg tttgctgttc gtcagattac ggaagccgcg ctcgccgtac gccagcccct
     1621 tccagccgtg ctgctccatc tcattgagca tcattttgcc gatctcgccg tcaagcactt
     1681 tgtacgcatg ctccggtgtc gagaagagga atggcagatc gaaaacaccg aacttcggca
     1741 tgaagttcat gatcggaccg ccggtgatga tggcggaatc aaccacgccc atgcgcatgc
     1801 tttcgaggag attccgctcg tcgccgagct gcgcgttggg gtatatcgtc accgtgagct
     1861 cccctttgct tcccgcctcg acaagctcct tgaacttcag cgcgcagaca tggaacgcgt
     1921 cattctcatt cacaacgtgc gcaagcttga tatccaccgc cgcgaccgcg gcggaagcga
     1981 caaagagcaa ggtcaaaagg acgatcgata caactgcgat acttctcttc atgatactat
     2041 cccctcctgg ttgtattgaa gatatgattg tgaaaaaacc gctcctcgga cacagcatcc
     2101 aaagaggagc attaagcctt ggcaacgcat tatagctatt agcgcgagaa ttgcaagtct
     2161 gtttcaatgt tttgcgtcca gtaaaccgaa tgttcattta aggaaacccg ctacagtctt
     2221 cgccgcacat atgtttgtga ttctgtttgt gattctggac aacgcttcgc ttgcctgcta
     2281 tcatcggaac agaccggcgt tcctctggca cattgaggac aatacgtaag gagagtggtt
     2341 atacaatggc cgattttgtg ctgctcgcag ggaaaatttc cgacatccgg ctggcggagg
     2401 acccatcgca tgcatcgggt atcgagaaac gaattgtctt cgcccccggg aaattctgga
     2461 acgactacgt tgcgcgccac tttaccgtaa agggcaaaaa ctcgaaaacg cctttccaca
     2521 cgcacgactg gccccattac gtgtttattt tatcgggaag atgcaacgcc acaattgacg
     2581 gcacggtcta tccgcttgaa aaagggtgct gggctcatgt tcctccgaat gtggaacact
     2641 ttttcgagag tacaggggaa gagccgttgg actttatctg cattgtgcct cccaagggcg
     2701 accccgcagg aagcgcccac ctcgcctgat tttcggcggg aggcagcctt tttacagcat
     2761 cccccaaagc agagagaggc cggaagcgtc gcttgccggc ctctctcttt ttaatgcgga
     2821 aaggaaaagt tcatcatcgg aggaagccac gggtacaccg accgaagaaa gtacgggttc
     2881 atagcaaaaa tgacaaacaa ccccatcgcc attgcggaat ggaacactga aagcaacccc
     2941 ctgtatttct ccttcttggc gacagcgcca agaaaccctt cgcagacaag gaacgccgtt
     3001 atgtaataca gccaaccgtt tgttcccggt tcgtgaagaa aaatcatcgt cctcagtacg
     3061 cagtaggcgg accaaatacc cagaatatac atctggcttc ctgtcattgc cagcttcgca
     3121 ataaaggcga ttgctctcgg aaaaagctcg ccgctctttt ccatagcttc aagcgtatcg
     3181 aaagccgatt cgatgaaggg aaggatataa cgaatatatc cgacggagag aatccatccc
     3241 caaaaaacaa gaaaaaaaac ataattaaga taggtcaaca aaatcccgcc ttccggagcg
     3301 tatgataact aaagcgctcg caatatactg cttccctgta ctctgcgaga cacgaaatga
     3361 aacggcgcac cctccaaccc gcctcttttt tccagagcgt ttgttcaggc ggccgtgagc
     3421 aaggcaggct acctctgttc cactatcggt ttcgcgtagg cggtttccgc atcccacgga
     3481 aagaggatcc acgtgtcctg actgacttcc gttatatagg tatccacgaa cgcacggccg
     3541 tccggttttg cgtacacagc ggcaaagtgg gctttcggga gcattcgtcg aacaacctgg
     3601 gcggtttttc ccgtatcgac gagatcgtcg atgataagcc accctgcccc gtcgccggag
     3661 atttccttta aaaccgaagg ctccccctgt gtgcgcagca cgtagctcga tacacagact
     3721 gtatcaacaa gccgaacatc aagctcccgg gcgatgattg ctgcggggat cagtccgccc
     3781 ctcgcaattc ctatgattct ttcccacttg cacacactga caagacgcca cgccaaggct
     3841 ttgcaatcgc gatgcacctg ctcccatgaa accggatagg tcttatgata tctttcactt
     3901 gacacgccac ccactcctcc ttcaattctt ctcgcgcagc cgactggtgt attgtagcat
     3961 gcccgtttcc ttctgaaaaa gtaaaatgct tcctctacat tttttttctt aagacgctac
     4021 tgtataatat ctaaggaggg gcgtacccct caattcttat attcagggag gttgaaagcg
     4081 tgaaaagatt cgttgtgctc ctcgtttttg ttttgagcgt cactctgtgc ggtgcggcgt
     4141 acgccgcgtt ccccgcgatc cccgccgaga agatcaaccc gggatttgtc tacataggcc
     4201 ccatcggcga cggcggatgg acctacatgc atgagcaggg ccgccttgcc atggagaagg
     4261 aattccccgg ggtgaagtcg acctttgtcg aatccattcc ggaaggcccc gacgcggcca
     4321 gagtcttcga gaccatgata cgcaacggct cgaaggtcat tttcggcacg actttcggct
     4381 acatggattt cgtccaggag gtcgcgaaga agtaccctga caccattttc atgcactgcg
     4441 cgggatacaa agacgccccg aacgtcggaa catatttcgg cagaatgtac caggctcgtt
     4501 atctctccgg cctcgtcgca gggaaaatga ccaagaaaaa ccgtatcggc tacgtcggcg
     4561 cacaccccat ccccgaggtc atcaggggaa tcaacgcgtt cacgcagggc gtccgcaagg
     4621 cgaaccccga ggccgaagtt caggtaatct ggattttctc ctggcttgac ccgggcaagg
     4681 aaaaggaagc cgcaaaagcg ctcatcgact ccggagtcga tatccttgca atgcacgcag
     4741 ataccggcgc gaccccgcag gcggctgaag aggcgggtat ctatgtcatc ggatataata
     4801 acgacatgag cgcctatgcg ccgacgaagc acctcaccgc gcccatatgg gattggggaa
     4861 tggtgtacaa ggagacgatc ggccaggtca tcaaggggac atggcagccc agccacatct
     4921 ggtacggact gaaagagggc atggtggctc tcgctcctta cggaaaggat gtacccgact
     4981 tggtgaaagc tctcgtcgac gtcgagaaag ccaagattgt gtcgggagag tgggatgtat
     5041 tccacggccc ggtgaaggat cagagcggcg aagtgaaggt tcctgaaggc gctgcaatga
     5101 ccgacggaga gatgctttcc atgaactggt ttgtagaagg cgtcaaggga gatataccca
     5161 aatagccctt tcttttgtat catatatggg ggaacttcgg ggcgcgaatt tccgcgcccc
     5221 tttttcttcc cgattcatca ctacattacc gatccagaac ggatacacat cctctccaca
     5281 gacactcttt tgagcggaga atccgtacac tggatcgcaa gggggaaaag ggatggatgc
     5341 acgagctcct tcgaaaccgc tcgtccgcat ggaaaaaatc accaaacgct ttacaggggt
     5401 gacggccaat ctcgacgtgg acttcgacgt ccgcccggga gaagt
//
LOCUS       DAOTIL010000062         5396 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminobacteriaceae bacterium isolate AMR_MDS_3026
            NODE_18018_length_5396_cov_3.253885, whole genome shotgun sequence.
ACCESSION   DAOTIL010000062 DAOTIL010000000
VERSION     DAOTIL010000062.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868050
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminobacteriaceae bacterium (wastewater metagenome)
  ORGANISM  Aminobacteriaceae bacterium
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae.
REFERENCE   1  (bases 1 to 5396)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5396)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:19:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,224
            CDSs (total)                      :: 3,198
            Genes (coding)                    :: 3,184
            CDSs (with protein)               :: 3,184
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5396
                     /organism="Aminobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18018_length_5396_cov_3.253885"
                     /isolate="AMR_MDS_3026"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:3034609"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..848
                     /gene="groEL"
                     /locus_tag="P5201_02175"
     CDS             <1..848
                     /gene="groEL"
                     /locus_tag="P5201_02175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008521962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="chaperonin GroEL"
                     /protein_id="HRV97368.1"
                     /translation="LRGVMQVAAVKAPGFGDRRKAMLQDIAILTGGQVVSEEIGIKLD
                     SAELSMLGKAKKVRITKEETTIVEGAGDSDEIRKRAAQIKREIEDSTSEYDKEKLQER
                     LAKLVGGVAVIQVGSATETEQKELKHRIEDALNATRAAVEEGIVAGGGVAPVSCVEAL
                     EKFVATLVGDERTGAVIVLKALTAPLHLIAENAGYQGDVVVEKVRGLKAGHGLNAVSG
                     DYVDMIEAGIIDPVKVTRSALQNAGSIAAMVLTTEAIVADKPEKKEPAMPGGMGGMGG
                     MGDMY"
     gene            1154..2671
                     /locus_tag="P5201_02180"
     CDS             1154..2671
                     /locus_tag="P5201_02180"
                     /EC_number="7.5.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter ATP-binding protein"
                     /protein_id="HRV97369.1"
                     /translation="MSEYIVELEGVGKSFAGIRALDDVSLNLKSGEVLALLGENGAGK
                     STLMKILGGIYTRDTGIIRVFGEEIDEMTPKRAKELGIAVIHQELNMCTHLTVAENIF
                     LGREILRSGMLDDREMNIRAAEVLSRLNIDIAPDTVVGSLPISKQQMVEIAKALSTNA
                     RILVMDEPTSALTSKEINDLFHIIRSLRDQGCGIIYISHRLEELQHIVDRVTILRDGK
                     YIATTRFCDTTMAEIISNMVGREIKEKFPRVETPLGRTILRVKGLNAGRQVRNVSFEL
                     REGEIVGVAGLMGAGRTEMTRALFGADPKDSGEVLLDERPLRIEKPMDAIEAGIVLVP
                     EDRKKDGLCTKLSIRENVALPNLDILKGALSTVNSRGEREMVERTVKNLSIRLSNAEQ
                     NADSLSGGNQQKLVVGKWLAREFRVVLFDEPTRGIDVAAKAEIYHLMNDLKTRGLGVL
                     FISSEMPEILGIADRIIVMCDGRVTGEFPAGTATQEKILECATRFEDKLGLAAGD"
     gene            2703..3725
                     /locus_tag="P5201_02185"
     CDS             2703..3725
                     /locus_tag="P5201_02185"
                     /inference="COORDINATES: protein motif:HMM:NF014684.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HRV97370.1"
                     /translation="MDDSKSPSRLGRLFAARGMGQVVTAAFGLVILCVVFGILNPIFF
                     SGRNVGNLLRQIAPILLIGIGQSYVLITGNIDLSIGSVVGMSCMVSATLMTMGMNPWV
                     AVAVTLFCCLMVGVLNGCLVAKFNLPPFIATLGTMTIARGIAQIVNNNYNTDSIGPAA
                     EGFRNFFYYGRLGGLFNTIWISLALWLVFNFILSRTRLGRHIYATGSNKEAARLSGVD
                     VVASTTAAYMVSAVCSCVVGLIVCATSGMGTMDAGNMYELYAVAASVIGGVSTLGGQG
                     LLLGTVMGASIWGVLQNGLQFAGAPVAIRNIVIGVIVVVSVLLDVVVRSEKRRRKRER
                     KEEAKG"
     gene            3839..4747
                     /locus_tag="P5201_02190"
     CDS             3839..4747
                     /locus_tag="P5201_02190"
                     /inference="COORDINATES: protein motif:HMM:NF024799.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HRV97371.1"
                     /translation="MKKAVAVFLGCVLLAGLFVGGAAAAEKHKVYLITMDQMDQHWVT
                     VNAGAEKAVAELGTIDYKWLAPDVKDDAKQIEAVNNAVAGGAEVLLVAANGPTAISAA
                     LKEASEAGVKIVYVDSPADFPAIQTLATDNEAAGVTAGKEMLKALEAAGVAEGKIGIV
                     NVNAATASTVAREKGFRAAFEGTKFELLETQYCDGDAARAKDIASNYITQGCVGIFGA
                     NEGSTVGTGNAIQEAGGKVVGVGFDKSNMILSLIKNGFLVATMAQNPDVMGYEGVKTA
                     AKALAGEPTGAEYVDTGVSVLTKNDL"
     gene            4852..>5396
                     /locus_tag="P5201_02195"
     CDS             4852..>5396
                     /locus_tag="P5201_02195"
                     /inference="COORDINATES: protein motif:HMM:NF012692.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HRV97372.1"
                     /translation="MYAIGVDIGGTKCAVCLGKARGDTLRVVHKCELRKTAEYSPAEM
                     LDALLADVRECRSRIPARETVIGTGISCGNPLDSRAGVILSPPNLPGWDRIEVVDWFR
                     SGTGLPAWLCNDANAGALAEWRYGAGRGCEHLIFLTFGTGFGAGLILNGRLYAGASDA
                     AGETGHVRAAAHGPVGYGKIGS"
BASE COUNT         1096 a         1556 c         1806 g          938 t
ORIGIN      
        1 agcttcgcgg cgtaatgcag gtcgcggccg ttaaggcccc cggcttcggc gatcgccgca
       61 aggccatgct tcaggacatc gcgatcctca ccggcggcca ggtcgtcagc gaagagatcg
      121 gcatcaagct cgacagcgcc gaactctcca tgctcggcaa ggcgaagaag gtccgtatca
      181 ccaaggaaga gaccaccatc gtggaaggcg ccggcgactc ggacgaaatc cgcaagcgcg
      241 ccgcccagat aaagagagag attgaggact ccacctccga gtacgacaag gagaagcttc
      301 aggagcgcct tgcgaagctc gtcggcggcg tggccgtcat ccaggtcggc tccgcgaccg
      361 agaccgagca gaaggaactc aagcaccgca tcgaggacgc cctcaacgcg acccgcgcgg
      421 cggtcgaaga gggtatcgtg gccggcggcg gcgtagcccc cgtgagctgc gtggaagccc
      481 ttgagaagtt tgtcgccacc cttgtcggcg acgagcgtac cggcgcagtc atcgtcctca
      541 aggcgcttac cgctcctctg cacctcattg ccgagaacgc cggctaccag ggcgacgtcg
      601 tcgtcgagaa ggtccgcggc ctgaaggccg gacacggcct caacgcggtc agcggcgact
      661 atgtcgacat gatcgaggcc ggaatcatcg atcccgtgaa ggtgacccgc agcgccctcc
      721 agaatgcggg ctccatcgcc gcgatggtcc tcaccaccga ggccatcgtc gccgacaagc
      781 ccgagaagaa agagcccgcc atgcccggcg gcatgggcgg aatgggcggc atgggcgata
      841 tgtactaaat ccgggcgtac agttcctcac gcttggcacg caaccctccg gagcctttgg
      901 ccccggaggg ttttttgtat gccggaaaag agatccgaat caagcgatga ttgctggaag
      961 ctcggtgtgt atgctacact atggaagcgt taaattaaga gtagacgcga gtgagttcac
     1021 aaaaaatgtt tcatttgatg gaattgtggc gagcgtatga tactcgggag aatcagcctt
     1081 cggaacgtgt ttttcctatg tggagcgtcc cggatacgtg ggttctccgg ctgtagcgag
     1141 cggaggtggc ggcgtgtcgg aatacatagt cgagcttgag ggcgtcggca aatccttcgc
     1201 cggaatcagg gcgctggacg acgtttcgtt gaacctgaag agcggggagg tgctcgcgct
     1261 tctgggcgag aacggcgccg gaaagtcgac gctgatgaag atcctcggcg gaatctatac
     1321 ccgcgacacc gggatcatcc gggtcttcgg ggaggagatc gacgagatga cgccgaagcg
     1381 ggcgaaagag ctgggcatcg ccgtcatcca tcaggagctg aacatgtgca cccacctcac
     1441 cgtcgcggag aacatctttc tcggcaggga gatcctccgt tccggtatgc tcgacgacag
     1501 ggagatgaac atccgcgccg ccgaggtcct ctcccggctg aacatcgata ttgctcccga
     1561 tacagtggtg ggatcccttc ccatctcgaa acagcagatg gtggagatcg ccaaggcgct
     1621 ctccacgaac gcgcggatcc tcgtcatgga cgagcccacc tccgcgctca cttccaagga
     1681 aatcaacgat ctgttccata tcatccgttc gctgcgcgac caggggtgcg gcatcatcta
     1741 tatctcgcac cggcttgaag agctgcagca tatcgtggac cgcgtgacca tcctccgcga
     1801 cggcaagtac atcgcgacga ccaggttctg cgataccacc atggcggaga tcatctcgaa
     1861 catggtggga cgcgaaatca aagagaagtt cccgagggtg gagacaccgc tcgggcggac
     1921 gatactccgg gtgaaggggc tgaacgcggg gcgtcaggtg cggaacgtga gcttcgagct
     1981 gcgcgaagga gagatcgtcg gcgtcgccgg actcatgggc gcaggcagga ccgagatgac
     2041 ccgcgcgctc tttggagcgg atccgaaaga cagcggggag gttttgctcg acgaacggcc
     2101 tctgcgaatt gaaaagccga tggacgcgat cgaggccggt atcgtcctcg tgccggagga
     2161 cagaaaaaag gacgggctct gcaccaagct gagcatccgc gagaatgtcg cccttccgaa
     2221 cctcgatatc ctcaagggag cgttgtcaac ggtgaacagc agaggcgagc gggagatggt
     2281 ggagcggacg gtgaagaacc tctcgatcag gctctcgaac gcggagcaga acgccgacag
     2341 cctctccggc ggcaaccagc agaagctggt ggtcggcaag tggctcgccc gcgagttccg
     2401 cgtggtcctc ttcgacgagc cgaccagggg gatcgacgtg gcggcgaagg cggagatata
     2461 ccatcttatg aacgacttga aaacaagagg gctcggcgtg ctcttcatct cctcggagat
     2521 gccggagatc ctcggtatcg cggacaggat tatcgtaatg tgcgacggcc gcgtgacggg
     2581 ggagttcccc gccgggactg cgacgcagga gaagatcctc gaatgcgcca cgcgcttcga
     2641 ggacaagctc ggccttgcgg ccggggattg attattctac acggggagcg atgactgtga
     2701 gcatggacga ttcgaaaagc ccctcccgcc tcgggcgtct tttcgcggcg agaggcatgg
     2761 gacaggttgt gaccgccgcg ttcggtctgg tgatcctctg cgtggtgttc ggcattctga
     2821 acccgatctt tttctccggg cgcaacgtcg ggaatttgct gcgccagata gcgccgattc
     2881 ttttgatcgg catcggccag tcctatgtgc tcatcaccgg caatatcgac ctctccatcg
     2941 gttccgtggt ggggatgagc tgcatggttt cggcgacgct gatgacgatg gggatgaatc
     3001 cgtgggtcgc cgttgccgtc acgcttttct gctgcctgat ggtgggcgtc ctcaacggat
     3061 gcctggtggc gaagttcaat ctccccccct tcatcgcgac gctggggacg atgaccattg
     3121 cgcgcggcat cgcgcagatc gtgaacaaca actacaacac cgactccata gggcctgccg
     3181 ccgaggggtt ccggaacttc ttctactacg gcaggctggg cggcttattc aataccatct
     3241 ggatttcgct cgcgctctgg ctggtcttca acttcatcct atcacgaacg cgactcgggc
     3301 ggcatatcta cgccaccgga agcaacaagg aggcggcgcg tctctcgggc gtcgacgtcg
     3361 ttgcgtcgac gacggccgcg tacatggtca gcgccgtctg ttcctgcgtc gtcgggctga
     3421 tcgtctgcgc caccagcggc atggggacga tggacgcggg caatatgtac gagctgtacg
     3481 ctgtggcggc ctcggtcatc ggcggcgtga gcacgctcgg aggacagggg ctgctgcttg
     3541 gaacggtgat gggcgcctcg atctggggcg tgctgcagaa cgggcttcag ttcgcagggg
     3601 cccccgtggc gatacgcaac atcgtcatcg gcgtgatcgt ggtcgtctcg gttcttctcg
     3661 acgtcgtcgt cagaagcgag aagcggcgca gaaaaaggga acggaaagaa gaggcgaagg
     3721 gctgatgctc ttccccggag cgaggcctgt tcgacgcagc gcttccgggg agagacggaa
     3781 ctctccgctt cttttgatac aacggtttca catcatatct tcaaggggga acggacgaat
     3841 gaaaaaagca gtagcggtgt ttctggggtg cgttttgctg gccggacttt ttgtcggcgg
     3901 cgcggcggcg gcggagaagc acaaggtata tctcatcacc atggaccaga tggaccagca
     3961 ctgggtgacg gtgaacgccg gagctgaaaa agcggtggcg gagctcggca ctatcgatta
     4021 caagtggctc gctcccgatg tgaaggacga cgccaaacag attgaagcgg tcaacaacgc
     4081 cgttgccggc ggagccgaag tgctcctcgt ggcggccaac ggaccgacgg cgatcagcgc
     4141 ggcgctgaaa gaggcttccg aggcgggcgt caagatcgtg tacgtcgact ctcctgcgga
     4201 cttccccgca atccagacgc tggcgacgga caacgaggcc gccggagtca ccgcgggcaa
     4261 ggagatgctg aaggcgcttg aagcggccgg agttgccgag ggcaagatcg gcatcgtcaa
     4321 cgtcaacgcg gccaccgcgt cgacggtcgc ccgcgagaag ggcttccgcg cggcgttcga
     4381 agggacgaag ttcgagctgc tcgagacgca gtactgcgac ggcgacgccg cacgggcgaa
     4441 ggacatcgcc tccaactaca tcacccaggg gtgcgtcggc atcttcggag ccaacgaggg
     4501 ctcgaccgtc ggaacaggca acgccattca ggaggccggc ggcaaagtgg tcggcgtggg
     4561 cttcgacaag tcgaacatga tcctcagcct gatcaagaac ggcttcctcg tcgccaccat
     4621 ggcgcagaac cccgacgtca tggggtacga aggcgtgaag accgcggcca aagctcttgc
     4681 aggagagccc acgggagccg agtatgtgga caccggcgtc tccgttctga cgaagaacga
     4741 cctgtagaag gagcgtaccg gagtatgaag aaggcccccg tccctcaggg cgggggcctt
     4801 ctgtagtagg agcgcccttg cgggagctcc cgtgagctcg gagggatggg aatgtacgcg
     4861 ataggcgtcg atattggagg aacgaagtgc gccgtatgcc tcgggaaggc cagaggagat
     4921 actttgcgcg tcgtgcacaa gtgcgagctc aggaagaccg cggagtactc gcctgcggag
     4981 atgctcgacg cactgctcgc tgacgttcgg gagtgcagat cgaggattcc ggcgagggaa
     5041 acggtcatcg gcacaggaat cagctgcggc aatcccctcg acagccgggc gggggtgata
     5101 ttgtcccccc cgaacctgcc cggttgggac cgcatagaag ttgtcgactg gttccggagc
     5161 gggaccgggc ttccggcgtg gctctgcaac gacgccaacg cgggtgcgct ggcggagtgg
     5221 cggtacggcg ccgggagggg gtgcgaacac ctgatcttcc tgaccttcgg caccgggttc
     5281 ggcgcggggc tgatcctcaa cggccgcctc tacgccggag cgagcgacgc cgcaggggag
     5341 acgggacacg tccgcgctgc ggcgcacggt cccgtcgggt acgggaaaat cgggtc
//
LOCUS       DAOTIL010000063         5371 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminobacteriaceae bacterium isolate AMR_MDS_3026
            NODE_18132_length_5371_cov_3.051919, whole genome shotgun sequence.
ACCESSION   DAOTIL010000063 DAOTIL010000000
VERSION     DAOTIL010000063.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868050
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminobacteriaceae bacterium (wastewater metagenome)
  ORGANISM  Aminobacteriaceae bacterium
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae.
REFERENCE   1  (bases 1 to 5371)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5371)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:19:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,224
            CDSs (total)                      :: 3,198
            Genes (coding)                    :: 3,184
            CDSs (with protein)               :: 3,184
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5371
                     /organism="Aminobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18132_length_5371_cov_3.051919"
                     /isolate="AMR_MDS_3026"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:3034609"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1311
                     /locus_tag="P5201_02200"
     CDS             <1..1311
                     /locus_tag="P5201_02200"
                     /inference="COORDINATES: protein motif:HMM:NF037272.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lectin like domain-containing protein"
                     /protein_id="HRV97373.1"
                     /translation="SCWSFASFVALESPLLPGEVRDFSEWHLAYWTFVTNPEGFPAFT
                     AWSDPTDPFSPDDPAKVYNQGGDDWKTVAVLVRGTGPVNEADLPYGGPLPSTKPNLPR
                     EKLLTGALYMPYSASEIWEQGYPYIVADEVKASIIKYGALSIGMMADFNENTWNTETH
                     AFFYNGPRGANHAVNVVGWDDGFPKENFATTPPGDGAWIVRNSWGEEFGEGGYFYMSY
                     YDTSVDSGIAYILASPDLYGIIHQHDPLGWVRSVSASEANPETGWMANIFTAGEDQTL
                     RAVSFYAAAYGTMYEVFIDDVSGGVSGSGEALRTAEASGTLEQPGYHTIPLPRPVGVG
                     EGTRFRVRVKLTTPGYEYPLPVELPLEEYSDNATASPGESFYSADGASWTDLTEDFAE
                     ANFCIKVLSTPGMSRSGGSGGCSAASASPLLFALLAPLLLLRRR"
     gene            complement(1402..3090)
                     /locus_tag="P5201_02205"
     CDS             complement(1402..3090)
                     /locus_tag="P5201_02205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012868859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HRV97374.1"
                     /translation="MEKKKVLIIGGVACGAKTAARLARLAPEFDITILERGEHLSVAG
                     CGFPYFVGDVVKEYKDLVCTPLGIVRDANFFRNVKNIAVHTGHLATRIDREKKVVAAT
                     DTASGEERLFPYDELVLATGASPIRPPIPGNDLSRVFTLWTMPDAMAMKSAIDAGSVK
                     SAVIIGGGLIGMEVVEALVDRGISVSVADIQPTPLPALIGEDFGLRVTKVLRQKGVGF
                     YGGEKVVEIIGENGVVTGVRTDKRTLPADMVLLAVGIRPNTILAKEAGLELHPNGTIV
                     VDECLRTSDPAIYAGGDCVQSKNILTEKSVWQPMGSTANRQGRVIADNIAELPTRFAG
                     VVGTAILKLFHLTIGKTGLNEEQAHAAGFDAVSIVVEEPDRPHFMPGMGAMTIRLVAD
                     RKSRRIVGAQIMGGGVVDKRLDSIVAAISAGMTVDMLADTDFAYAPPFATALDPTTHA
                     ANALRNKMDGLVKTYSAKELKALMEGANPPFLLDVRSPQELDLQGRIPYDFANIPLGQ
                     LRGRITEIPKDREIVTFCKISVRGWDAIATLRGNGYENVALLEGGIMAWPYDLR"
     gene            complement(3207..3437)
                     /locus_tag="P5201_02210"
     CDS             complement(3207..3437)
                     /locus_tag="P5201_02210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014806464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3343 domain-containing protein"
                     /protein_id="HRV97375.1"
                     /translation="MHCLATFDITSMAVLFERVCRKEGITAKIVPVPRSLSTSCGLAC
                     EFPCEEEERVRELCGKKHVDVTDFHSLGDDWT"
     gene            3585..3761
                     /locus_tag="P5201_02215"
     CDS             3585..3761
                     /locus_tag="P5201_02215"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRV97376.1"
                     /translation="MRFLLLALVLIAACIPMLLGSERVRPCYKGCHGCGKCMKSFRGD
                     EESEERTPQPPANR"
     gene            complement(3839..5134)
                     /gene="larC"
                     /locus_tag="P5201_02220"
     CDS             complement(3839..5134)
                     /gene="larC"
                     /locus_tag="P5201_02220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013049168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nickel pincer cofactor biosynthesis protein
                     LarC"
                     /protein_id="HRV97377.1"
                     /translation="MKTLYLDCFAGIAGDMFLGAMLDLGLDREAFLRTMESVVLFPGH
                     EHGHAHPDPHDHEHEHSHEHNDTHHHAHHHSHGTDRLRIAITQTVRGGIAGTKVNIAS
                     SEDHPHRGLADVLEIIGASPLSPRVKERSAAAFRLLAEAEAKVHGTTPDKVHFHEVGA
                     LDSIADIIGAFVLVEMAGVDTVVSSPLNVGHGTITCAHGILPVPAPATAELLAGLPVR
                     VEGGPMERVTPTGALLVRCLASSFGPLPAGKVLGSGKGAGDRDGDIPNVLRVLLLETE
                     ATNTDGAGNIARDSGIVIETNIDDMNPQYYAPVMNRLLAEGALDVWVAPIVMKKGRPA
                     ITLGCLCRPGAERSLAETLLRETTTLGVRMYAVDRLKADAEIVERETSWGIVRFKVAR
                     LGEEVLRSNPEFEDVRRLSEEHGIPLPEMRERLMNEFLL"
BASE COUNT          956 a         1626 c         1729 g         1060 t
ORIGIN      
        1 tcctgctgga gcttcgcctc cttcgtcgcc ctggagtcgc ctcttctgcc cggagaagtc
       61 cgggattttt ctgaatggca tctggcatat tggacgtttg tgacgaaccc cgaaggcttc
      121 cccgcattta ctgcgtggag cgacccgacc gatccattct cccccgacga tccggcgaaa
      181 gtctacaatc aggggggcga cgactggaag accgtggcgg tgcttgtcag agggacgggg
      241 ccggtgaacg aggccgacct cccttacgga gggcctcttc cgtcgacgaa accgaatctt
      301 ccgagggaaa aactgctgac cggagcgctt tacatgccct actctgcaag tgaaatctgg
      361 gagcaggggt acccgtatat cgtcgcggac gaagtgaagg cgtccatcat aaagtacggc
      421 gcgctctcca tcggcatgat ggcggacttc aacgagaata cgtggaatac tgaaacgcac
      481 gcttttttct acaacggccc ccgtggtgcg aatcacgcgg tgaacgtggt cggatgggac
      541 gacggtttcc cgaaggagaa cttcgcgacg acgcctccgg gagacggtgc gtggattgtc
      601 cggaacagct ggggagaaga attcggcgag ggcgggtact tctacatgtc ctactacgat
      661 acgagcgtcg acagcgggat cgcgtacatt ctcgcttctc ctgatctgta cggcatcatc
      721 caccagcacg atccgttggg gtgggtgcgc tcggtgagcg cctcggaggc gaatcccgag
      781 acagggtgga tggcgaacat tttcaccgcc ggggaggatc agacgctccg cgcggtttcg
      841 ttctacgcgg cggcgtacgg aacgatgtac gaggtgttca tcgacgacgt atcgggcggc
      901 gtctccggtt cgggggaagc gcttcgtacg gcggaggctt ccggtaccct ggagcaaccg
      961 ggataccata cgatccctct gccccggccg gtcggagtgg gagaaggaac gcgcttccgc
     1021 gtgcgggtga agctcaccac gccggggtac gagtaccccc tgcccgtcga acttccgctt
     1081 gaggagtatt cggacaacgc gacggcctcc cccggcgaga gcttctacag cgcggacggc
     1141 gcttcgtgga cggatctaac ggaggacttc gcagaggcga acttctgcat caaggtcctc
     1201 tcgacgcccg ggatgagcag aagcggcggg agcggcggat gctccgccgc ctccgcgtcg
     1261 ccgctgcttt tcgcgctgct ggcgcctctt ctgctgctcc gcaggaggta gagccaaaag
     1321 gctgttacgc aaaaagaaga tgcgggttcc cggagttctc gggggcccgc atcttctctt
     1381 tgcatgtaat actcgggttt tctaccgtaa atcgtacggc caggccatga tgccgccttc
     1441 gaggagggcg acgttctcgt agccgttccc gcgaagcgtg gcgatggcgt cccatccgcg
     1501 cacggagatc ttgcagaacg tcacgatctc gcggtccttc gggatttccg ttatacggcc
     1561 gcgaagctgt cccagcggga tgttcgcgaa gtcgtagggg atgcgcccct gaaggtcgag
     1621 ctcctgcggg gagcgaacgt cgagcaggaa cggagggttc gccccctcca tgagcgcctt
     1681 cagctccttc gccgaatagg tcttcaccag cccgtccatc ttgttccgca gcgcgttggc
     1741 ggcgtgcgtc gtggggtcga gcgcggtggc gaaggggggg gcgtaggcga agtcggtgtc
     1801 ggcgagcatg tcgaccgtca tcccggcgga gatcgccgcg acgatggagt caagccgttt
     1861 gtccaccacg ccgccgccca tgatctgcgc gccgacgatg cggcgtgact ttctgtcggc
     1921 aacgaggcgg atcgtcatcg cgcccatgcc cggcatgaag tgcggccggt cgggctcctc
     1981 gacgacgata ctcacggcgt cgaatcccgc cgcgtgcgcc tgttcctcgt tgaggccggt
     2041 ctttccgatg gtgaggtgga agagcttcag gatcgccgtg ccgaccactc cggcgaaacg
     2101 ggtgggcagt tccgcgatat tgtcggcaat gacgcgtcct tgccggttcg cggtggagcc
     2161 catcggctgc cagacggact tctcggtaag gatattcttg gactgaacgc agtcgcctcc
     2221 ggcgtagatg gccgggtcgc tcgtgcgaag gcattcgtcg acgacgatgg tcccgttggg
     2281 gtggagttcg agcccggctt ctttggcgag tatcgtgttc gggcgaatgc cgacggccag
     2341 cagaaccatg tcggcgggaa gggtgcgctt gtcggttctg acgccggtga cgacgccgtt
     2401 ctcgcctatg atttcgacga ccttctcgcc gccgtaaaag ccgacgccct tctgacgcag
     2461 cactttcgtc acgcggaggc cgaaatcctc tccgatgagg gccggaaggg gggtgggttg
     2521 gatgtcggct acggagaccg agatgccgcg gtccacaagc gcctcgacga cttccatgcc
     2581 gatgagcccc ccgccgatga tgacggcgga cttgacgctt ccggcgtcga tagccgactt
     2641 catcgccatc gcgtcgggca tcgtccagag ggtgaagacg cggctgaggt cgtttccggg
     2701 aatgggagga cggatggggg aggcgccggt ggcgagtacg agctcgtcgt aggggaagag
     2761 acgctcctct ccggaggcgg tgtcggtagc ggcgacgacc ttcttttccc tgtcgatgcg
     2821 cgtcgccaag tgtcccgtgt ggacggcgat attcttcacg ttgcggaaga agttcgcgtc
     2881 gcggacgatg ccgagcggcg tgcagacaag atccttgtac tccttcacca cgtcgccgac
     2941 gaaatacggg aagccgcatc cggcgacgct cagatgttcg ccccgctcca gaatggtgat
     3001 gtcaaactcc ggcgcaagtc gcgcgagccg ggccgccgtc ttcgcgccgc atgccacgcc
     3061 gccgatgatg aggaccttct ttttctccat gaccacaatc ccctgccttt cggaaattcg
     3121 cggcccggcg gacgtatgtt cagcgagcgg acgccctgac gccgcgattt atactgctac
     3181 ccctatatgg tatttcaaaa aggaagttat gtccagtcgt cccccaaaga atggaaatcg
     3241 gtcacgtcca catgtttttt cccgcagagc tcgcggacgc gctcttcctc ttcgcagggg
     3301 aattcgcagg caagtccgca gcttgtggaa aggctgcgcg ggacggggac aattttggcg
     3361 gtaattccct ctttgcggca gactcgctcg aagaggacgg ccatgctcgt gatgtcgaag
     3421 gtcgccagac agtgcatcgc taccgcctcc tttcgggatg aggaatgtgc cggcaaatgg
     3481 ccggttcgtg cgtcctggtt cgttcaaagc gcatgcaaac acaacctttc aaaaagagcg
     3541 tatactatgg gctgaaaaaa actcttttcc ggaggtgacg tcgtgtgcgt tttcttctgc
     3601 tcgcgctggt gctgatagcc gcctgtatcc cgatgctgct gggatcggag cgggtccgcc
     3661 cctgctacaa ggggtgtcac ggctgcggaa agtgcatgaa gtccttccgc ggcgacgaag
     3721 agagcgagga gcgcacaccg cagcctcccg cgaatcgctg acgactgctg cggacgcgac
     3781 ggcgttccgg actcctgaac agacggctct ctccggtccg aatgcggcgc gccgcttttc
     3841 agaggaggaa ctcgttcatc aatcgttccc gcatctccgg aagcgggatg ccgtgttcct
     3901 ccgaaagtct ccggacgtcc tcgaactcag ggttgctccg aagaacttcc tctccgaggc
     3961 gcgccacctt gaagcggacg ataccccacg aggtctcccg ctccacgatt tccgcgtcgg
     4021 ccttcagcct gtcgaccgcg tacatccgga cgccgagcgt cgtcgtctcc cgcagcagcg
     4081 tttccgccaa gctccgctcc gcgccgggcc ggcagaggca gccgagcgtt attgcggggc
     4141 gtcctttttt catgacgatg ggcgcaaccc atacgtcgag cgccccctcc gcgaggagcc
     4201 tattcataac cggcgcgtaa tactgcgggt tcatgtcgtc gatgttcgtc tcgatgacga
     4261 ttcccgaatc ccgcgcgata ttccccgcgc cgtcagtgtt ggtcgcttcg gtctcaagca
     4321 gcagcacgcg cagtacgttg gggatgtcgc cgtcgcggtc gcccgccccc ttgccgcttc
     4381 cgagcacctt ccccgcgggg agcggcccga acgaggaagc gaggcatcgc acgagcagcg
     4441 ctccggttgg ggtgacgcgc tccatcggcc ctccttcgac gcggaccgga agaccggcaa
     4501 gcagctctgc ggtcgccgga gccgggacgg gaaggatgcc gtgcgcgcat gtgatggttc
     4561 cgtgcccgac gttcagcggc gaggagacga ctgtgtccac gcccgccatc tccacgagga
     4621 cgaacgcgcc gatgatgtcg gcgatcgagt ccaatgcccc gacttcatgg aagtgtacct
     4681 tgtccggagt cgttccgtgg accttcgcct cggcctcggc caaaagacga aaggcggcgg
     4741 cgctccgctc cttcacacgc ggagagagcg gacttgcccc gatgatttcg aggacgtcgg
     4801 cgagccctcg gtgcggatga tcctcggacg aggcgatgtt cacctttgtt cccgcgatgc
     4861 cgccgcggac cgtctgcgta atggcgatgc gcagcctgtc cgtgccgtgc gaatgatggt
     4921 gtgcatgatg gtgtgtatcg ttgtgttcgt ggctatgctc gtgttcgtga tcgtgcgggt
     4981 cggggtgcgc atgaccgtgc tcgtggccgg gaaagagcac gacgctttcc atcgtgcgga
     5041 gaaacgcctc cctgtcgagc ccgaggtcga gcatcgcgcc gaggaacata tcgccggcga
     5101 ttccggcgaa acagtcaaga tagagtgttt tcatcaaaag acctccgtgt gcaaagttca
     5161 caacgcggtt attgtatcat ctgccggacg gaatacatcg ggaggtgaga aaaacggcgc
     5221 gccgtcccca cgcaaaaagc cgccgggcga acggatacat ccggcggcgc tgtgttctcc
     5281 ttcacgagaa aaggtcagtg gatttcgatc tccatatcct tcgtgcactg cttcccggaa
     5341 gtgttgtcgg tgaccaccca ggagagaata t
//
LOCUS       DAOTIL010000064         5329 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminobacteriaceae bacterium isolate AMR_MDS_3026
            NODE_18322_length_5329_cov_4.143345, whole genome shotgun sequence.
ACCESSION   DAOTIL010000064 DAOTIL010000000
VERSION     DAOTIL010000064.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868050
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminobacteriaceae bacterium (wastewater metagenome)
  ORGANISM  Aminobacteriaceae bacterium
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae.
REFERENCE   1  (bases 1 to 5329)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5329)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:19:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,224
            CDSs (total)                      :: 3,198
            Genes (coding)                    :: 3,184
            CDSs (with protein)               :: 3,184
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5329
                     /organism="Aminobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18322_length_5329_cov_4.143345"
                     /isolate="AMR_MDS_3026"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:3034609"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..255)
                     /locus_tag="P5201_02225"
     CDS             complement(<1..255)
                     /locus_tag="P5201_02225"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRV97378.1"
                     /translation="MMTLFSSKKKKTFVKTSASSRGRGKRENSSVLSLWIETLTFLIF
                     AFLLYVLASIFSLRTGVWGDAIRFSLLRNWGGAILIPVFFG"
     gene            complement(233..1615)
                     /locus_tag="P5201_02230"
     CDS             complement(233..1615)
                     /locus_tag="P5201_02230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008711654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TldD/PmbA family protein"
                     /protein_id="HRV97379.1"
                     /translation="MSINAKLGDILRTVTRTGADFADMYFEIAASHSYAFEDGSFEEI
                     SSSSSEGVGARILLGEATSLAYAPGVALETGASCLHEAAEHSGLSLPCRRMPAIRVME
                     REPPFPTPEYAFFYDLDKRIKASSMWIRQVSMNLDTAFKSFSVFNSDGSIAGDRRLYT
                     IFSVEVVVEKNGILQTGYESEAFSVDSGEFFRTVSPFKVAQRALSRALLMLDAPQCPA
                     GAMTVLLSGEAGGTMIHEACGHGFEADIIQKDYSVYRDKIGKQAASSLITLVDDGSIP
                     GYLGSGSFDDEGTPCKRNVLIEKGVVRSYISDIASSRRGALPLTGNGRRSSYRSVPQP
                     RMTNTYIEPGDSSPVEMLHSMNRGLLVKKMGGGEVDPTSGDFVFHVTEGYLVEGGRVL
                     HPVRGAVLTGNGPDVLMNIEAVGNDLHFLPGMCGKSGQSVPVTDGQPSLLVGNLTVGG
                     ANTCDDAIFE"
     gene            complement(1612..1896)
                     /locus_tag="P5201_02235"
     CDS             complement(1612..1896)
                     /locus_tag="P5201_02235"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRV97380.1"
                     /translation="MSWIVSEYDGLGFVKTENAAVEKYMGNAVGSSSKEREAYVSLFF
                     PEKKRDDVLELLGVLKNEGLEIALFFELPPSPYTSKDSAKERTLEDFDTL"
     gene            complement(1977..2993)
                     /gene="mltG"
                     /locus_tag="P5201_02240"
     CDS             complement(1977..2993)
                     /gene="mltG"
                     /locus_tag="P5201_02240"
                     /inference="COORDINATES: protein motif:HMM:TIGR00247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008932 - lytic endotransglycosylase
                     activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endolytic transglycosylase MltG"
                     /protein_id="HRV97381.1"
                     /translation="MKKILCFFLAAAGLFMLIRTTAFSPERWPLPLPNGSGELLPVMV
                     QTGMNARQAADEFVAAGIVEKGRAASFARWMVRFSIDRRLRPGVYHVRKGSPWEVAMQ
                     MKTAEPGFLSLTLVPGTDIYSFGTLFPDKSLSGESLEKLLADESLYPSEMRNLLPETT
                     EGRLAFLLPETYHLAFLNERELVSAAAALWWRRIGSSIPPEQRSDEVFEELAILASLV
                     EREGFRDDERPRIAGVLSNRMEKGMQLQIDATVVYAWRRKGRDITRVLFKDLEIDSPY
                     NTYKIQGLPPAPICTSSEISWKAVLNPEQHDFLYYVAQSDGYHLFAETFSEHQKNIVK
                     VRSK"
     gene            complement(3019..4035)
                     /gene="amrS"
                     /locus_tag="P5201_02245"
     CDS             complement(3019..4035)
                     /gene="amrS"
                     /locus_tag="P5201_02245"
                     /inference="COORDINATES: protein motif:HMM:TIGR04337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AmmeMemoRadiSam system radical SAM enzyme"
                     /protein_id="HRV97382.1"
                     /translation="MKNAEGSKETVREAEWWRFEGEDVRCMLCPHVCLISPAKAGRCG
                     VRIHVKGEGLRLSNYGMAASLAADPIEKKPLYHWKPGTSVLSLGSVGCSMHCPFCQNW
                     QLAECSASVSLQRIAPEDPARLAMQHGTPSVAFTYNEPLTWFEFLKDSCRLLREEKIP
                     VVLVSNGMINAAPLEEIVPYIAAANIDLKAFTPDAYRVMGGELDAVKRTIRTLSAAGT
                     HVEVTFLLVPGINDDAFSFAEMASWLASLHPVPVLHISRYFPNREWRVPATPLERIKE
                     FERIAHERLPWVYPGNIDGESCTRCLECGRVLLRRRRYTLLEKNIDEKGRCAFCESPT
                     PVTL"
     gene            complement(4032..>5329)
                     /gene="amrA"
                     /locus_tag="P5201_02250"
     CDS             complement(4032..>5329)
                     /gene="amrA"
                     /locus_tag="P5201_02250"
                     /inference="COORDINATES: protein motif:HMM:NF014899.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="AmmeMemoRadiSam system protein A"
                     /protein_id="HRV97383.1"
                     /translation="HPPVIVPEVGRGRERDAFQTLEGMNAVSSLASTNPPDTILLLSP
                     HAPFSGGLTISLAEQYSGNFAQFAAPRTRLSLRGASEGGEGLVRTLEKSFPVVATRRK
                     ECPLDHGSLVPLFFLQKSGIPLPSLVVANPIGLSPSEAFRLGQALLKAPLHGSWALVA
                     SGDLSHRVTPDAPAGYTPFGREFDRMVVEAFLTNDPAQLLALGEREMDEAGECGLRSA
                     LLFLGLGEKRQIRSLSYEAPFGVGYATAFASLHSAPLLARTVLSEGVRSGREAAKRAA
                     ASFHEFPELQKRSACFVSLKIRPGRTEDEQLRGCIGTILPSSSSLHEEIVENTLAAAQ
                     RDPRFMPVTPEELENISLSVDILSTPEPVSSLDMLDPRKYGVLVEKNGLRGVLLPDLE
                     GVDTVEQQLSIAARKAGILSLERASLSRFTVRRLKEDAE"
BASE COUNT         1316 a         1508 c         1389 g         1116 t
ORIGIN      
        1 cccgaaaaaa acaggaatga gtatagcgcc gccccagttt cgcagtagag aaaaacggat
       61 cgcatccccc caaacgcctg ttctgaggga aaagatcgaa gcgagcacat agagaagaaa
      121 ggcgaagatc aaaaaagtga gcgtttctat ccaaagcgac agtacgctgg agttttctct
      181 cttgcctctt cctctggaac tcgcagacgt tttgacgaac gtcttctttt ttttactcga
      241 aaatagcgtc atcacacgtg tttgcccctc cgacggtgag atttccaacg agcagggatg
      301 gctgtccatc ggtaacaggt acgctctgcc ctgactttcc gcacatccct ggcaggaagt
      361 gaagatcatt ccccactgcc tcgatgttca taaggacatc cgggccgttt ccagtaagca
      421 ccgctccacg tacagggtga agaactcttc ctccctcgac gagataccct tctgtaacat
      481 gaaagacaaa atctcctgat gtaggatcca cttctccgcc gcccattttc ttgacgagaa
      541 gacctctgtt catagaatgc agcatctcca caggcgagga atctcccggt tctatatacg
      601 tgtttgtcat ccgcggctgc ggaacggagc gataggaact ccgcctgccg tttccagtca
      661 ggggaagggc gcctcgtctc gacgaggcga tgtcgctgat atagctccgg acaaccccct
      721 tttcgataag aacgtttcgt ttgcagggag tgccttcatc gtcaaaggaa ccgcttccga
      781 gataccccgg aatagaaccg tcgtcgacaa gcgtgatcaa cgagctcgcg gcctgtttgc
      841 ctatcttgtc ccggtaaacg gaatagtcct tctgaatgat gtccgcttcg aacccgtgcc
      901 cgcaggcttc atggatcata gtgcctcctg cttctcccga gagcagcacc gtcattgcgc
      961 ctgcagggca ctgaggggcg tcgagcataa ggagagcacg agagagcgcc ctctgcgcca
     1021 ctttgaacgg agaaactgtc ctgaagaact cgcctgagtc cactgagaag gcttcagatt
     1081 catacccggt ttgcagtatg ccgttttttt caacgacaac ctctacgctg aaaatcgtat
     1141 aaagacgccg gtctccggcg atagagccat cgctgttaaa aacgctgaaa ctcttaaacg
     1201 ccgtgtcgag attcatggat acctgccgta tccacataga gctcgccttt atacgtttat
     1261 ccagatcata aaaaaaggca tactccggag taggaaaggg cggctccctc tccatgacgc
     1321 gaatcgcagg catccggcga caaggaaggg aaagaccgct gtgttccgcc gcttcatgaa
     1381 ggcaggaagc gcccgtttcc agggcgactc ccggcgcgta cgcaagagaa gtcgcttcgc
     1441 ccagaagaat ccgtgcgcca actccttcgg aagaagacga cgaaatctct tcaaagctgc
     1501 cgtcctcaaa tgcatacgag tgcgacgccg ctatctcaaa atacatatcg gcaaaatcgg
     1561 cgccggtgcg cgtcacggtc cgcaatatat ctcctagttt tgcgttgatg ctcatagcgt
     1621 atcgaaatcc tccaatgtac gttctttcgc cgaatccttc gaggtatatg gagaaggcgg
     1681 caactcgaaa aaaagagcta tctccaaacc ttcattttta aggactccta gtaattctaa
     1741 cacgtcatcc cgtttcttct ccggaaaaaa gagagaaaca taggcctctc tctccttgga
     1801 ggagcttccg acagcattcc ccatatattt ttcaaccgca gcgttttctg tcttgacgaa
     1861 tccaaggcca tcgtattcgg atacgatcca gctcaagagc acaatctgcg cagcaggcag
     1921 cccaagcccg aaacacccca tatcaggagc gaaggagaca gacgggggag gaaacatcac
     1981 ttggatcgaa cctttacgat gtttttctgg tgctctgaaa aagtttcggc aaacaggtga
     2041 tagccgtccg actgggcaac ataatagaga aaatcatgct gttcaggatt gagaacagcc
     2101 ttccaggaga tctcggagga ggtgcatatc ggtgcaggag gaagaccttg tattttatac
     2161 gtattatagg gagagtctat ttccaggtcc ttaaagagca cgcgcgttat atcccgtccc
     2221 tttcttctcc atgcatagac gactgttgcg tcgatttgga gctgcatccc cttctccatt
     2281 ctattggaaa gtacgccggc gatgcgcgga cgttcgtcgt cccggaatcc ttcacgttct
     2341 acaagggaag cgaggatcgc caattcctca aaaacctcgt cgctcctctg ctccggtggt
     2401 attgaggaac ctattcgtct ccaccagagc gcggcagccg cagagaccag ctctctttca
     2461 ttcaagaaag cgaggtgata cgtttcggga aggagaaagg cgagccggcc ttcagttgtt
     2521 tcggggagca aattgcgcat ttccgatgga tacaggcttt catccgccaa cagtttttcc
     2581 agcgactctc cggaaagtga cttgtccggg aagagcgtgc cgaaagaata gatatcggta
     2641 ccgggtacaa gcgtcaaaga aagaaagccc ggctctgcag ttttcatttg catcgctact
     2701 tcccaaggag agcccttccg aacatggtac actccgggcc gcagacgacg gtcgatggaa
     2761 aagcgcacca tccagcgagc aaatgaggcg gcccggccct tctcaacaat gcccgccgcc
     2821 acaaactcat ccgctgcctg ccgcgcgttc atccccgtct ggaccatcac cggaagcagt
     2881 tcgccgcttc cgttcgggag aggaagcggc caccgttcgg gagagaacgc agtggtacgg
     2941 atcaacatga agagtccggc agcagcgagg aaaaaacaca gtattttttt catagaaatg
     3001 cattcctcct agagacagct aaagagtgac cggcgtcggc gactcgcaga aagcgcaccg
     3061 ccccttttcg tcaatattct tttcaagaag agtatagcgg cgtcttcgaa gcaaaactcg
     3121 gccgcactcc aaacaacgcg tacaactctc tccgtcgata tttccgggat acacccacgg
     3181 aagtcgttca tgtgcaatac gctcgaactc ctttatacgt tccaaaggag tcgcgggaac
     3241 ccgccattct cgattcggga agtagcggga aatatggagc acaggaaccg ggtgaagaga
     3301 agcgagccaa gacgccatct ccgcaaagga gaaagcgtca tcgttgattc caggcacaag
     3361 gagaaaggtg acttctacgt gcgttcccgc ggccgaaaga gtcctgatcg tcctttttac
     3421 cgcgtcgagc tctcccccca taacacggta cgcgtccggt gtaaaggctt tcaagtcaat
     3481 gtttgcggcg gcaatatacg ggacgatctc ctccaatgga gctgcgttga tcattccgtt
     3541 gcttaccagc acgacgggga tcttttcctc tcgaaggaga cggcaggaat ccttgagaaa
     3601 ctcgaaccag gtcaaaggct cattgtaggt gaacgcgacg gagggcgttc cgtgctgcat
     3661 cgcgaggcgc gcgggatcct caggggctat cctctgcaat gaaaccgacg ctgaacactc
     3721 cgcaagctgc cagttctgac agaagggaca gtgcatcgaa caccctacgg aaccgaggga
     3781 cagtaccgaa gttcccggct tccagtgata gagaggtttt ttttcgatgg gatccgctgc
     3841 gagggaggcc gccatgccgt aattcgagag gcgaagacct tctcccttca catggatacg
     3901 cacgccgcaa cgccccgcct tcgccggaga aatgagacac acatgaggac agagcataca
     3961 ccgtacatct tctccttcga agcgccacca ctcagcctca cgcactgttt ctttcgaacc
     4021 ctccgcgttc ttcactccgc atcctccttg agcctgcgaa cagtaaaacg agagagagac
     4081 gccctctcca gagagagtat tcccgctttc cgggcagcta ttgaaagctg ctgttcgacg
     4141 gtatctactc cctccagatc agggagcaag acccctcgca aaccgttctt ttcgacgagc
     4201 acaccgtatt tacgcggatc gagcatgtca agagaggaca ccggctccgg agttgagagt
     4261 atgtcgactg aaagagaaat gttctcgagc tcttccgggg ttacaggcat gaaacgaggg
     4321 tcgcgctgcg ccgcggcaag cgtgttctcc actatctctt catgaagcga agaagaggaa
     4381 ggaagaatcg ttccgataca cccgcgcagc tgttcatcct ctgttctccc cggacgaatc
     4441 ttcaggctga cgaaacaggc ggatctcttc tgaagctccg ggaattcatg aaaactcgcc
     4501 gccgctcttt ttgcagcctc tcgtccggaa cgcacacctt cggagaggac tgtacgcgcc
     4561 agcaacggcg cgctgtgcaa cgatgcaaag gcagtcgcgt acccaacgcc gaagggagcc
     4621 tcgtatgaga gactccgtat ttgccgtttc tcgcccaacc cgaggaaaag gagcgctgaa
     4681 cgcaaaccgc attctccggc ctcgtccatt tcgcgttctc caagagcgag gagctgtgcg
     4741 ggatcgttcg ttagaaaagc ttcgacaacc atcctgtcga actcgcgccc gaaaggggtg
     4801 tagccggcgg gagcatcagg ggtaaccctg tgcgaaaggt cgccgcttgc gacaagcgcc
     4861 cagctcccat gaagaggcgc cttcaggaga gcctgtccca gccggaacgc ctcggaagga
     4921 gaaagcccga tcggatttgc gacaacaagt gagggaagag gaatgccgct cttttggagg
     4981 aaaaaaaggg gaacgaggct cccgtgatcg agcgggcact cctttcgcct ggtcgctact
     5041 acagggaacg atttctccag agttcgaaca aggccttcgc ccccctctga ggcccctctc
     5101 aaagaaagac gagtccgggg agccgcaaac tgtgcaaaat tgcccgaata ctgctcggcc
     5161 agcgagatcg tgagccctcc tgaaaaaggc gcgtgcggag agagcagaag aatggtgtca
     5221 ggcggattcg tgctcgcaag ggaggaaacc gcgttcattc cttcgagcgt ttgaaaggca
     5281 tcgcgctctc ttcccctacc gacctccggg acaatcaccg gaggatgag
//
LOCUS       DAOTIL010000065         5327 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminobacteriaceae bacterium isolate AMR_MDS_3026
            NODE_18328_length_5327_cov_4.444044, whole genome shotgun sequence.
ACCESSION   DAOTIL010000065 DAOTIL010000000
VERSION     DAOTIL010000065.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868050
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminobacteriaceae bacterium (wastewater metagenome)
  ORGANISM  Aminobacteriaceae bacterium
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae.
REFERENCE   1  (bases 1 to 5327)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5327)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:19:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,224
            CDSs (total)                      :: 3,198
            Genes (coding)                    :: 3,184
            CDSs (with protein)               :: 3,184
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5327
                     /organism="Aminobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18328_length_5327_cov_4.444044"
                     /isolate="AMR_MDS_3026"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:3034609"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..529)
                     /locus_tag="P5201_02255"
     CDS             complement(<1..529)
                     /locus_tag="P5201_02255"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HRV97384.1"
                     /translation="MTKRRLPVRVLVAEDDPMVCSLHVQTIQQVHGFTVAGTVGDGIE
                     MVRFFESSSADLIVMDIFMPRLSGLDALKKLRAEGNVADVILVSAGKHPQLVSQAKLL
                     GAFDYLIKPYSLKRFRTSLEAYLENRTKMPSETEEATQEALDSLFEIASANRSPLGSE
                     KLPKGLQPGTLSLVMK"
     gene            839..1009
                     /locus_tag="P5201_02260"
     CDS             839..1009
                     /locus_tag="P5201_02260"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRV97385.1"
                     /translation="MTNFAQSVAPYILYTCSLVLLPLGVNRFRAGSPVKGFLDFVGAG
                     VIAVMAWSLANP"
     gene            1129..2106
                     /locus_tag="P5201_02265"
     CDS             1129..2106
                     /locus_tag="P5201_02265"
                     /inference="COORDINATES: protein motif:HMM:NF024545.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HRV97386.1"
                     /translation="MNRFRKVCVSSLLLLVLLASVAVATETKPEPAKAEPSPAAVEEK
                     APVADPETVLATVGSAEIKQKDVTAIIGRLDPQRAAMYDNEMGRRAILEELVNMELFS
                     LLGAELAVEKDPKFIETMNEVRKDVTRQFAVEAIMKDVAVTDEEIAAYYEKNKENFKV
                     PETVRASHILVADEDEMKKVRADLDAGMSFEDAARKHSSCPSKEQGGDLGYFSKEQMV
                     PEFGNAAYAMKVGDISAEPVKTQFGLHLIKLVDKKEASVRPLDEVRAQLTESLLNEKK
                     AGVYNEQLAKLREKHQVVIVGEKKEEAKVEAKEEAKEEPKEEAKKEEKK"
     gene            complement(2192..3424)
                     /locus_tag="P5201_02270"
     CDS             complement(2192..3424)
                     /locus_tag="P5201_02270"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HRV97387.1"
                     /translation="MNGEQTIPELEERIVFLTMERRAAMEALEVALDISSFTASLTGI
                     ESVEEILRESAVKIRALLPFEALAFFLADEDDDMALAPAYADPPEKMHVIEQEKKPLI
                     DDRTVAWALRRKKPVIVATQDRSGRIFLHALASPSRVLGLFIGILGKEEEAQQTPSYP
                     DFLSIVLSATSGVIGTNMLLRKINLLNLALQEKVHFLEKSKEELSLYRNNLEREVAAR
                     TKELARRNEDLRREIVERKRMESEIRYRAWHDALTGLANRELFHSRLLEAVAKGEPIA
                     VFFMDLDGFKNINDTLGHDSGDSLLQEAARRIAREMGEENTVARMGGDEFTVIISFPT
                     SRELLAECAERLLHGISEPISLEGASVSITASIGISRFPEDGATTQDLMKCADVAMYK
                     AKERGKNRYCFWSDIRGK"
     gene            3586..3717
                     /locus_tag="P5201_02275"
     CDS             3586..3717
                     /locus_tag="P5201_02275"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRV97388.1"
                     /translation="MSEVLRRKGRVLSNASLRPLFEKTGAGKALLQALRATKYGGGL"
     gene            3717..4235
                     /locus_tag="P5201_02280"
     CDS             3717..4235
                     /locus_tag="P5201_02280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013047496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5698 domain-containing protein"
                     /protein_id="HRV97389.1"
                     /translation="MDYVGLLLIFCARIVDVSCGTVRILFLVRGRRALAAMIGFVEVM
                     IYLTALGHIIGGGREMNWIQMVVYCAGFSTGNFIGSLLEEKLMNAYVFLELIMDRTAE
                     TKEMIDNIRADGFGATVIYGRGRDGVRTIVKIICRRRDVPVIVAHTHNKGFICISDVK
                     GCSGGQFRFQRK"
     gene            4281..>5327
                     /locus_tag="P5201_02285"
     CDS             4281..>5327
                     /locus_tag="P5201_02285"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain-containing protein"
                     /protein_id="HRV97390.1"
                     /translation="MKDSLGVVRERNVGEIGGRQAGTCPEGYCEKTLRLEKELTRWKR
                     EEAVLRDIERRYLALLDSPLLILLILSEGRVLFMNRRGEEFFGFALRERPRFLLSEYA
                     ASGYASSVESLFTPGSDGAVRGGRLDFAVRSESGRERRIDCAFVPGVYQGAPVLLGAG
                     YELPEGGEEGSETDSVDVLLSEREDLLFCSMDTEYVPTFMTEGFRNASVRIWGRSPDQ
                     NEALLKILPSGEHGESFRLAAERAHAGEYAETAFEAGELVYSCSFAPLYSKEGEPLGI
                     SLLLTDRTELCRLERERYAEAESFQRLFASSGEMMLIASVEDGKLLRGSPAFLSWMGY
                     EETAILGRTESDFAL"
BASE COUNT         1102 a         1376 c         1596 g         1253 t
ORIGIN      
        1 ctttcattac aagggagagc gttcccggct gcagcccctt ggggagcttc tcgctcccca
       61 agggagatct gtttgcggag gcaatttcga agagagaatc cagcgcctcc tgggtcgctt
      121 cctcagtctc cgacggcatc tttgtcctgt tttcaaggta ggcttcaaga gatgtccgga
      181 agcgtttcag cgaatagggc ttgatcaggt agtcgaacgc tccaagcagc ttggcctggg
      241 ataccagttg cggatgcttg ccggccgata caagaatgac gtcggcgacg ttcccctcgg
      301 cgcggagttt tttcagcgcg tcgagaccgg aaagacgggg catgaaaata tccatgacga
      361 taagatcggc tgaagacgac tcgaaaaaac gcaccatctc aatgccgtcg ccgacggttc
      421 cagctacggt aaagccgtgc acttgctgaa tcgtctgtac atgaaggctg cataccatcg
      481 gatcgtcctc ggctacgaga actctgacgg gtagtcttcg tttcgtcatt ttcctcaccc
      541 cttgattgaa ttatatgctc tcgctccgcg ttcgtaaaca caatgaacaa aaagaaaatc
      601 aatgaacaaa actcttgctt tttttaggtt tgtgattcag aatgaatggt acataaattg
      661 cattcccggt tcggttgatg aacttcccca cgcactggtt ccgcttcccg aaaacctgcc
      721 ccgggttttc gacgaggagc ggaaccccct tttttctctc tggcttcgga aacgctcatc
      781 ccgcttgact ttcttctcca tgcactgcat tattaacacg tccaaaggag gggtctcgat
      841 gacgaatttt gcgcagtcag tcgcgccgta catcctgtat acgtgttcgc tcgttcttct
      901 tccgctcggg gtcaatcgct tcagggcggg cagtcctgtg aaggggtttc tcgatttcgt
      961 cggcgcgggg gtcattgccg tcatggcctg gagtcttgcg aacccctagg cgctgatggc
     1021 atgaaacggg ttttcagcat tttgtacgtt tctttttccc ttttctatgg tacactctgt
     1081 ccgttttgtt ccaaattccg tgaaggatca ttttagggag gtttttgaat gaatcgtttc
     1141 agaaaagtat gcgtgtcatc gctgttgctc cttgttcttc tggcgtccgt cgcagttgcg
     1201 accgagacga agccggaacc ggcaaaggcg gagccgtctc ctgcggcagt cgaagagaag
     1261 gccccggttg ctgatcccga gaccgtgctc gctactgtcg gatcggccga gatcaagcag
     1321 aaggatgtta ccgcgattat cggccgtctc gatccccaga gggcggccat gtacgacaac
     1381 gagatggggc gcagagcgat tctcgaggaa ctggtcaaca tggagctctt ctctctgctc
     1441 ggggccgagc tcgctgtgga gaaggatccg aagttcatcg agaccatgaa cgaagtccgg
     1501 aaggacgtca cgcgccagtt cgcagtcgag gcgatcatga aagacgtcgc cgtcaccgac
     1561 gaggagatcg ccgcgtacta cgagaagaat aaggagaact ttaaggttcc cgagaccgtc
     1621 cgcgcgagcc atattctggt cgccgacgag gacgagatga agaaggtgcg cgcggatctc
     1681 gacgcgggaa tgtccttcga ggatgctgcg cggaaacact cttcctgccc ttcgaaggag
     1741 cagggcggcg acctgggcta cttctccaag gagcagatgg tgccggagtt tggcaacgcc
     1801 gcctacgcca tgaaggtcgg cgacatctct gccgaacctg taaagacgca gtttggtctg
     1861 catctgatca agcttgtcga taagaaagag gcctccgtac gccccctcga cgaagtccgg
     1921 gcgcagctta ccgagtctct cctgaacgaa aagaaggccg gcgtctacaa cgaacagctg
     1981 gcgaagctcc gtgagaagca ccaggttgtc atcgtgggag agaagaaaga agaggccaaa
     2041 gtagaggcaa aagaagaggc aaaagaagag cccaaagaag aggccaaaaa agaagagaag
     2101 aagtaattca aagctgcctc gtagatttgg agaataaggg cagggggccg tgcagcatcg
     2161 gttcccgccc tttttttcgc tcacgttccg gtcattttcc cctgatatcg gaccagaagc
     2221 agtacctgtt ttttccgcgc tctttggctt tgtacatggc tacgtctgcg catttcatca
     2281 agtcctgcgt ggtggcgccg tcctccggga agcggctgat gccgatgctc gcagtgatgg
     2341 agacggaggc cccttcgagg ctgatcggtt ccgatatgcc atgcagcagc ctttcggcgc
     2401 actcggcgag cagttcccgc gatgtgggaa aggagatgat cactgtgaat tcatctccgc
     2461 ccatccgtgc aacggtattc tcttctccca tttcccgggc aatcctcctg gcagcttctt
     2521 gcagcaggct atcgccgctg tcgtgtccga gggtgtcgtt gatgttcttg aagccgtcga
     2581 gatccataaa gaagacggca atcggttctc cctttgcgac cgcttcgagc aggcgggagt
     2641 gaaagagttc tctgttcgca aggcctgtaa gcgcgtcgtg ccacgcgcga tagcggatct
     2701 ccgattccat acgtttcctt tcgacaattt cccggcgaag gtcttcgttt cttctggcga
     2761 gttcctttgt tcgtgcggct acttctcttt ccaggttgtt gcgatagagc gacagctctt
     2821 ccttcgactt ttccagaaag tgaacttttt cctggagcgc gagattgagc agattgattt
     2881 ttcgaagaag catgttcgtg ccgatcacgc cggatgttgc cgacaggacg atggagagaa
     2941 agtcggggta ggaaggcgtt tgttgtgctt cttcttcctt cccgagaatc ccgataaaga
     3001 ggccgagtac tcgcgagggc gaggcgagag catgaaggaa aattctgccg gagcggtctt
     3061 gcgttgcaac gatgaccggt ttctttctgc gaagcgccca cgcgacggtg cggtcgtcga
     3121 taagcggttt tttttcctgc tcgatgacgt gcattttttc cggcggatcg gcgtacgcgg
     3181 gagcgagcgc catgtcgtcg tcctcgtcgg cgaggaagaa tgccagcgcc tcgaacggga
     3241 ggagcgcgcg tatttttacc gcggactccc ggaggatctc ttccacggac tctatgccgg
     3301 taagcgacgc ggtgaaggag ctgatgtcca gggcgacctc aagcgcctcc attgcggcgc
     3361 gacgctccat ggtgagaaaa acgatccgtt cttcgagctc ggggatggtc tgttcgccgt
     3421 tcatagcgtc tctcctttta cgcgaaagaa ttgcgttttt gctcatttgc cgtttattat
     3481 actctgccgg agaggtatga taggatagta acggtatgtt gtatcaaaaa ttgctcgtcc
     3541 cggcatgcac tgagtgtctt taccgggaag caaggaggtt tcggaatgtc tgaagtgttg
     3601 cgaaggaagg gacgcgttct ttcaaacgcc tctcttcgtc ctctctttga aaagaccggc
     3661 gccgggaaag ctcttctgca ggcgctccgg gcgaccaagt acggaggagg gctctgatgg
     3721 attatgtcgg acttcttctt atcttctgcg cccgtattgt cgacgtaagc tgcggaacgg
     3781 tgcggattct cttcctcgtc agagggagaa gagcgctggc ggcgatgatc ggctttgtcg
     3841 aagtgatgat ctatcttacc gcccttgggc acatcatcgg gggcggacga gaaatgaact
     3901 ggatccagat ggtggtctac tgcgccggtt tttccacggg aaatttcatc ggctccctgc
     3961 tggaagaaaa actgatgaac gcctatgtct tcctggaact cataatggac cgaacggccg
     4021 agacgaaaga gatgatcgac aatatccgtg cggacggttt cggggcgacg gtgatctacg
     4081 gccgaggtag ggacggcgtc cgcaccatcg tgaagataat ctgccgcaga agggacgtac
     4141 ctgtcatcgt cgcgcacaca cataataaag ggttcatctg cataagcgac gtcaaggggt
     4201 gctccggcgg gcagttccgt ttccaacgca agtaactttg gttcataccc ctggttcacg
     4261 gaaggaaggg atggggcttc atgaaggaca gtctgggagt tgttcgcgag aggaacgtcg
     4321 gagagatcgg ggggcgtcaa gcgggaacat gtccggaggg gtactgcgaa aagacccttc
     4381 ggctggaaaa agagttgacc cgctggaaac gcgaggaggc cgttctgcgc gacattgaac
     4441 ggcgctatct tgcgctgctt gacagcccgc tcctgatcct gctgatcctc tccgagggga
     4501 gagtgctctt tatgaacagg cgcggcgagg agtttttcgg atttgcgctt cgcgaacgcc
     4561 cccgatttct cctttcggag tatgcagcgt ccggatatgc ttcatcggta gagagcctct
     4621 tcactcccgg ttccgacggc gccgttcgcg gagggcgtct cgacttcgcc gttcgttcgg
     4681 agagcggacg ggagcggaga atcgactgcg cgtttgtccc cggcgtgtac cagggagcgc
     4741 cggtgctcct gggggcgggg tatgaactcc cggagggcgg ggaggaaggc tcggagaccg
     4801 attctgtcga cgtgctgctc tcggaaagag aagatcttct tttctgttcc atggatacgg
     4861 agtatgttcc gacgtttatg acggagggtt tcagaaacgc ttccgttcgt atctggggga
     4921 ggagtcccga ccagaatgaa gcgcttttga agattctccc ctccggtgag catggagaat
     4981 cgttccgcct cgcggccgag agagcgcatg ccggagagta tgctgaaacc gcttttgagg
     5041 ctggagaact cgtgtattcc tgttccttcg ctccgctcta cagcaaggaa ggggagccgc
     5101 tcggaatttc gttgcttctg accgacagaa cagaactctg ccggctggag agggagcggt
     5161 acgccgaggc ggagagcttt caacgtcttt tcgcctcctc cggggagatg atgctgatcg
     5221 cctccgtgga ggacgggaag ttgcttcggg gatctcccgc gtttctctca tggatggggt
     5281 atgaggagac ggcgatcttg gggcgcacgg agtcggattt tgctctg
//
LOCUS       DAOTIL010000066         5327 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminobacteriaceae bacterium isolate AMR_MDS_3026
            NODE_18329_length_5327_cov_3.360584, whole genome shotgun sequence.
ACCESSION   DAOTIL010000066 DAOTIL010000000
VERSION     DAOTIL010000066.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868050
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminobacteriaceae bacterium (wastewater metagenome)
  ORGANISM  Aminobacteriaceae bacterium
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae.
REFERENCE   1  (bases 1 to 5327)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5327)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:19:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,224
            CDSs (total)                      :: 3,198
            Genes (coding)                    :: 3,184
            CDSs (with protein)               :: 3,184
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5327
                     /organism="Aminobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18329_length_5327_cov_3.360584"
                     /isolate="AMR_MDS_3026"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:3034609"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..286)
                     /locus_tag="P5201_02290"
     CDS             complement(<1..286)
                     /locus_tag="P5201_02290"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRV97391.1"
                     /translation="MRTLSAETSYSRRLDALFKHPATDAAMLVLISMSVAVAAGSLLV
                     DNEELEHLNLYFSLLFSVELTARCFTYASNKREYFADCWMDWIATIPWDIF"
     gene            complement(332..1810)
                     /locus_tag="P5201_02295"
     CDS             complement(332..1810)
                     /locus_tag="P5201_02295"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HRV97392.1"
                     /translation="MRTVRRKSLFLSVFILTAFILSHVPSETAVRPLKQSYAPDEPIE
                     IVYDNLPGSQTDWISLAPRENADDRYGEWFYTGGMRSGSHTFGGLPAGVYEARVYHDW
                     PAGGYVVQERAAVFVQEAVPEQSGPVPAEIPAAMRPTPRPEPHPSPVPEPVEISWSAS
                     GQGTLFAPGQGVHNYRIHELDARRFLNGGTLIVDVRIGAGQSWASVDLYGDGQSLATS
                     GVPQALASAWDLPPNSNRRITHTFRKGQIFRLCFEGNWGSPQGATNTYTYSATIKPAP
                     EHEPQLPPLPERETPHVAPPENAVNRRAAETLWRQAVSFQQQKRYEEALAKYREGLAL
                     SEDLAVAHHADAMETCLLRLSTLPDIAATRPEAAEIDIFNNWNKTAVVNNPAGPPSPT
                     RFTISRPHAITYINTYHYFNNGTPAGSITLVHQDGTLYGPWPATGRSGQGGVQNAIWE
                     ATPQTTLKAGTYTVFDSHPSTWSHNAQSDLRGFALVKGRLLQ"
     assembly_gap    1916..1925
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(2002..2520)
                     /locus_tag="P5201_02300"
     CDS             complement(2002..2520)
                     /locus_tag="P5201_02300"
                     /inference="COORDINATES: protein motif:HMM:NF012578.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HRV97393.1"
                     /translation="MAPDGGIHSEVTRMFWFGKKNLKLLAPLSGRVVPLEEVPDKVFA
                     GKMLGDGVAIDPDDDGLVVAPCDGDLVALFPTGHAFGIRGHKGIEVLVHIGIETVALE
                     GEGFTLIAKAGSKVKAGDPIVRFDKAIVAAKAPSILTPVIITTGDRVKSFKAASGTVR
                     AGKDVLLDIEPA"
     gene            complement(2487..3476)
                     /locus_tag="P5201_02305"
     CDS             complement(2487..3476)
                     /locus_tag="P5201_02305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006584168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRV97394.1"
                     /translation="MVRRILDANASDLAKMTGRELLTSIRAAEGRTLVAETIAPAPPL
                     LGDVTNAELARAFGADIILLNMFDVRAPHIEGLDVLNDDDFFLRTYGQGHRVRATETT
                     RAQTGTIAALKTLIGRPIAINLEPVDEGERVLGPQAKLPEGRRATPENARLAVEQGVD
                     MLVVTGNPATGVTTSSIAASLRGIREAVGPDPILAAGKMHAAGSATDAGSDIVDEAII
                     GRWIDAGADIVLLPAPGTVPGISPERVRDLIGVVHAKGALAMTAIGTSQEGADEATIR
                     QIALWAKSCGADLHHIGDSGYPGIAVPENILAYSIVIRGRRHAWRRMAASIAR"
     gene            complement(3514..4251)
                     /gene="nagB"
                     /locus_tag="P5201_02310"
     CDS             complement(3514..4251)
                     /gene="nagB"
                     /locus_tag="P5201_02310"
                     /EC_number="3.5.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012868991.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004342 - glucosamine-6-phosphate
                     deaminase activity [Evidence IEA];
                     GO_process: GO:0006046 - N-acetylglucosamine catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucosamine-6-phosphate deaminase"
                     /protein_id="HRV97395.1"
                     /translation="MRIVTAKDYDQMSRKASYIVASRVILNPKCVLGLATGDTPKGLY
                     NELVRLFRAGDVDFSGVTTFNLDEYAGLSPENPQSYHSYMEEHLFRHINIPPEHRHIP
                     NGAAQNIGAECRRYEELIEQAHGIDLQILGLGRDGHIGFNEPDVKFEKGTHHVRLTQS
                     TIEANARFFENRDEVPKSAISMGIKTIMSARRILLLASGPEKAEAIRGAVMGAVTPDL
                     PASVLQLHPNATLIVDEAASAEIMRIK"
     gene            complement(4223..>5327)
                     /gene="nagA"
                     /locus_tag="P5201_02315"
     CDS             complement(4223..>5327)
                     /gene="nagA"
                     /locus_tag="P5201_02315"
                     /EC_number="3.5.1.25"
                     /inference="COORDINATES: protein motif:HMM:TIGR00221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008448 - N-acetylglucosamine-6-phosphate
                     deacetylase activity [Evidence IEA];
                     GO_process: GO:0006040 - amino sugar metabolic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="N-acetylglucosamine-6-phosphate deacetylase"
                     /protein_id="HRV97396.1"
                     /translation="MVLLFDGRVLDILPEGELPAGVETLDAEGAWIVPGFVDIHVHGV
                     CGVDTMDGAAESLSAIAGGLAAHGVTAFCPTTMTMDEARIEAALDTVKAAKNAPPDGA
                     RVLGAHLEGPYISPSRLGAQDGTHLRRPDPEFVRRRKSALRLVTFAPEEDTDGRFLEA
                     LREEGIVASLGHSQASYEQAMKAFRNGAKSVTHLFNGMPPFHHRTPGLAGAALDADVF
                     CELIADGVHSHTAVFRLLLKMKGVERIVLVSDAMRGACLGEGEWDLGGQTVTVHGPEA
                     KLADGTLAGSVLTLDLALRNFAAATGLPLWEATRLVSENPARLLGERDRGRIEPGYRA
                     DFVLLDEGWRVLKTFVEGKEVFDRHENRDCKGL"
BASE COUNT          853 a         1661 c         1767 g         1036 t
ORIGIN      
        1 ggaatatatc ccacggaatg gtggcgatcc agtccatcca acagtcggcg aagtactctc
       61 gcttattcga cgcgtaggtg aagcagcgcg ctgtcagttc caccgagaag aggagcgaga
      121 aatagaggtt gaggtgttcg agctcttcgt tgtcgacaag cagcgatcct gccgcgaccg
      181 cgacggacat cgatataaga acgagcatcg ccgcgtccgt cgcggggtgc ttgaagagcg
      241 cgtccagccg ccgtgaatag cttgtttccg cggaaagagt ccgcatgaca tctcctcctt
      301 ctcataccac cggagcatac accggtagaa tctactgaag caggcggcct tttacgagcg
      361 cgaagccgcg cagatcggac tgcgcgttgt gggaccacgt cgacgggtgc gagtcgaaga
      421 cggtgtacgt cccggctttc agcgtcgtct gcggggtcgc ctcccatatc gcgttctgta
      481 cgcctccctg cccggagcgg cccgtcgcgg gccacggtcc gtagagcgtg ccgtcctggt
      541 ggacgagcgt gatgcttccg gccggagttc cgttgttgaa gtagtggtac gtattgatgt
      601 aggtgatcgc gtgcggccgg gagatcgtga agcgcgtcgg cgagggcggc ccggcggggt
      661 tgttcaccac ggcggttttg ttccagttgt tgaagatatc aatttctgcc gcttccggac
      721 gggttgcggc gatgtccgga agagtgctca gccgcaggag gcatgtctcc atcgcgtcgg
      781 catggtgtgc gaccgccaga tcctcggaga gagcgagccc ttcccggtat ttcgccagcg
      841 cctcttcgta ccgtttctgt tgctggaagg agacggcctg ccgccagagc gtctccgcag
      901 cgcgccggtt taccgcgttt tctggcggag cgacgtgcgg tgtttcacgc tcggggaggg
      961 gaggaagttg cggctcgtgc tcgggagcgg gcttgatggt cgcgctgtac gtataggtgt
     1021 tggtggcgcc ctgcggcgat ccccagttcc cctcgaagca gagacgaaag atttgccctt
     1081 tccggaaagt atgcgttatg cgccggttcg agttcggagg cagatcccat gcgctcgcaa
     1141 gggcctgcgg cactccagag gtcgcgaggg actgaccgtc cccgtacagg tcgaccgacg
     1201 cccagctctg ccccgcgccg atgcggacgt cgacgatcag cgtaccgccg ttgaggaagc
     1261 gtctggcgtc cagttcgtgg atgcgatagt tatggacgcc ctgtccggga gcgaagagcg
     1321 ttccctgtcc cgacgcggac catgagatct cgacgggttc ggggacgggg gagggatggg
     1381 gctccgggcg aggagtcgga cgcattgccg ctggaatctc ggccggaacc ggaccggact
     1441 gctccggaac ggcttcctga acgaagacgg cggcgcgttc ctgtacaacg tacccgcccg
     1501 ccggccagtc atggtagact ctcgcctcgt agactcctgc gggcaatccg ccgaaagtat
     1561 ggctcccgga gcgcatgccg ccggtgtaga accactcgcc gtaccggtcg tcggcattct
     1621 cccgcggagc aagcgaaatc cagtcggtct ggcttccggg aaggttgtcg tagacgatct
     1681 cgataggttc gtccggcgcg taggactgct tgagcgggcg gacggcggtc tcggaaggca
     1741 cgtgtgacag aatgaatgct gtcagtatga aaacggagag aaaaagagac tttcgtcgta
     1801 cggtacgcat agagtgtact ccccctcgct cgtgtgaata gtgaccccat gataccagaa
     1861 gcagggcttt tttcgcaaaa gaggagcgtt ttcatttttg gagaaaggtc cgcggnnnnn
     1921 nnnnngctct tcgagcagtc ttcagctaac ggagagaggg ggatgcggtg tggttccccc
     1981 tctttccgtc cttttcagcg cctacgccgg ttctatgtcc aggaggacgt cctttcccgc
     2041 gcggaccgtg ccggaggcgg ccttgaagga ttttacacgg tcgcccgtgg tgatgatgac
     2101 cggcgtgagg atggaaggag ccttcgctgc gacgattgcc ttgtcgaagc ggacgatcgg
     2161 gtcgcccgcc ttcactttgc tccccgcttt tgcgatcagc gtgaaccctt ctccctcgag
     2221 cgccactgtt tcgatgccga tgtgcacgag gacttctatt cccttgtgtc ccctgatgcc
     2281 gaaggcgtgc ccggtcggaa agagcgcgac cagatccccg tcgcatgggg cgacgaccag
     2341 cccgtcgtcg tcagggtcga tagccacgcc gtcgccgagc atcttcccgg cgaagacctt
     2401 gtcaggaacc tcctccagcg gaaccacccg tccggagagg ggagccagca gtttcaaatt
     2461 tttcttgccg aaccagaaca tgcgcgtcac ctcgctatgg atgccgccat ccggcgccat
     2521 gcgtgacggc ggccccggat gacgatggag tatgccaaaa tgttttccgg cacggcgatg
     2581 cccgggtagc ccgagtcgcc tatgtggtgc agatccgcgc cgcaactctt ggcccagagc
     2641 gcgatctgcc ggatggtcgc ctcgtccgcc ccctcctgcg aggtgccgat tgcggtcatt
     2701 gccagcgccc ccttggcgtg gacgacgccg atgaggtcgc gcacccgctc gggcgagatt
     2761 cccggaacgg tgccgggggc ggggaggagc acgatgtcgg cgcccgcgtc gatccaccgg
     2821 ccgatgatcg cctcgtcgac gatgtcgctt cccgcgtcgg tagcggagcc tgccgcgtgc
     2881 attttccccg cggcgaggat cgggtcgggc ccgacggcct cgcggattcc ccggagcgac
     2941 gcggcgatgg agcttgtggt gacgcccgtc gcggggtttc ccgtgacgac gagcatgtct
     3001 accccctgtt cgacggcgag acgggcgttt tccggcgtgg cgcgccgccc ctcggggagc
     3061 ttcgcctgcg ggccgagcac gcgctctcct tcgtcgaccg gttcgaggtt gatcgctatg
     3121 gggcggccga taagtgtttt gagcgccgcg atggttccgg tctgagcacg ggtcgtttcg
     3181 gtcgcccgca ccctatgtcc ctggccgtac gtgcgcagga aaaaatcatc gtcgttcagt
     3241 acgtccagcc cttcgatgtg gggagcgcgg acgtcgaaca tgttcagcag gatgatgtcg
     3301 gcgccgaaag cgcgtgccaa ctcggcgttt gttacgtcgc caagcagcgg gggggcgggg
     3361 gcgatggtct cggcgacgag cgtgcgtccc tccgccgcgc ggatgctcgt cagcagctcc
     3421 cgccccgtca tcttcgcgag gtcggaggcg ttggcgtcca gaattcgtcg taccatgatg
     3481 atgctccttt cctgagtaca gcagttctgc cggtcacttg atgcgcatga tttcggcgct
     3541 tgccgcttcg tccacgatga gcgtcgcgtt ggggtgcagc tggagcaccg acgcgggcag
     3601 gtcgggggtc accgccccca tgaccgcgcc gcggatggcc tccgccttct ccggtcccga
     3661 ggcgagcagc aaaatccgcc gcgcgctcat gatcgtcttg atccccatgc tgatggcgct
     3721 cttgggcacc tcgtcgcggt tctcgaagaa acgggcgttc gcctctatgg tgctctgcgt
     3781 gagacggacg tggtgcgtcc ccttctcgaa cttcacgtcc ggctcgttga agccgatatg
     3841 cccgtcccga ccgagtccca ggatctggag gtcgatgccg tgcgcctgtt cgatgagttc
     3901 ctcgtagcgg cggcactccg cgccgatgtt ctgcgccgcg ccgttgggaa tatgccggtg
     3961 ctccggcggg atattgatgt gccggaaaag atgttcctcc atgtagctgt ggtagctctg
     4021 cgggttctcc ggcgagagcc ccgcgtattc gtccaggttg aacgtggtga cgccggagaa
     4081 gtcgacgtcc cctgcccgga agaggcggac cagttcgtta tagagcccct tgggggtgtc
     4141 ccccgtggcg agcccgagca cgcacttcgg gttcaggatg acacggctgg cgacgatgta
     4201 cgacgccttc cggctcatct ggtcataatc ctttgcagtc acgattctca tggcggtcga
     4261 agacctcctt tccttccacg aatgtcttga ggacgcgcca cccttcgtcg aggaggacga
     4321 agtcggcccg gtaccccggc tcgatgcgcc ccctgtcccg ctcgcccagc aatcgggcgg
     4381 ggttctccga gacgaggcgc gtcgcctccc agagggggag gccggttgcg gccgcaaagt
     4441 tccgcagcgc gagatcgagc gtgaggacgc ttccggcgag cgttccgtcg gcaagcttcg
     4501 cctccggacc gtggaccgtg accgtctggc ctcccaggtc ccactcgccc tcgccgaggc
     4561 aggcgccgcg catggcgtcg gagacgagaa cgatgcgctc gacgcccttc atcttcagca
     4621 gcagccggaa gacggccgtg tgcgagtgaa cgccgtcggc gatcagctcg cagaagacgt
     4681 ccgcgtcgag cgcggctccc gcgagaccgg gggttctgtg gtggaatggc ggcatgccgt
     4741 tgaaaaggtg cgtcacgctc ttcgccccgt tgcggaacgc cttcatcgcc tgctcgtaac
     4801 tcgcctgcga atggccgagc gaagcgacga tcccctcttc ccggagcgct tccaggaaac
     4861 gaccgtccgt atcttcttcg ggagcgaacg tgacaaggcg cagggcgctc tttctgcggc
     4921 gcacgaactc cgggtcggga cggcgcaggt gcgttccgtc ctgcgcgccg aggcgggacg
     4981 ggctgatgta ggggccttcg aggtgcgcgc ccagcaccct cgctccgtcc ggaggcgcat
     5041 ttttcgccgc tttcactgtg tcgagcgccg cctcgatgcg cgcttcgtcc atcgtcatgg
     5101 tggtcgggca gaaggcagtg acgccgtgtg ctgccagccc tcccgcgatg gccgagagcg
     5161 attcggccgc cccgtccatc gtgtccacgc cgcagacgcc gtgcacgtgg atgtccacga
     5221 atccggggac gatccacgcc ccctccgcgt cgagcgtttc gacacccgca ggaagctcgc
     5281 cctcagggag gatatcgaga accctcccgt cgaagagaag aaccata
//
LOCUS       DAOTIL010000067         5320 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminobacteriaceae bacterium isolate AMR_MDS_3026
            NODE_18360_length_5320_cov_4.134473, whole genome shotgun sequence.
ACCESSION   DAOTIL010000067 DAOTIL010000000
VERSION     DAOTIL010000067.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868050
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminobacteriaceae bacterium (wastewater metagenome)
  ORGANISM  Aminobacteriaceae bacterium
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae.
REFERENCE   1  (bases 1 to 5320)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5320)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:19:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,224
            CDSs (total)                      :: 3,198
            Genes (coding)                    :: 3,184
            CDSs (with protein)               :: 3,184
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5320
                     /organism="Aminobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18360_length_5320_cov_4.134473"
                     /isolate="AMR_MDS_3026"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:3034609"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..350
                     /locus_tag="P5201_02320"
     CDS             <1..350
                     /locus_tag="P5201_02320"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HRV97397.1"
                     /translation="AVENIVRNALRYTPQHTTGQDGGKVRVSLFRVPETERPLHILVE
                     DEGPGVPPGELETIFIPFRRVERARERISASEGTGLGLAIAHRAAALHGGVISARNRP
                     EGGLAVEIELPGT"
     gene            complement(412..1083)
                     /locus_tag="P5201_02325"
     CDS             complement(412..1083)
                     /locus_tag="P5201_02325"
                     /inference="COORDINATES: protein motif:HMM:NF022732.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3089 domain-containing protein"
                     /protein_id="HRV97398.1"
                     /translation="MKRIHLLRAAVIAALLFTFAGNAAGESAEYSDPARWLSLPSYTL
                     MDTDIFYLYPTACRNEEAAIRGVDDPCMAEGALELFERELPVFEKLGDVYAPYYSQAS
                     AYRSSLPRERRRELVNGAPRRDVFAAFDYYMEHYNLGRPFILVGSCQGAEVLIQLLSE
                     YISKDSPIYGRMRAAYLSDCDVPEDFLLSNPHLRLARTPDGDGFVISSAGDVSAVPGS
                     ELQKQ"
     gene            complement(1186..2631)
                     /gene="cls"
                     /locus_tag="P5201_02330"
     CDS             complement(1186..2631)
                     /gene="cls"
                     /locus_tag="P5201_02330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014806881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008808 - cardiolipin synthase activity
                     [Evidence IEA];
                     GO_process: GO:0032049 - cardiolipin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin synthase"
                     /protein_id="HRV97399.1"
                     /translation="MTVTMLLYSAYELSSIAIRLIMLCYIPLKHTPSTATAWLLAIYF
                     WPWLGFLLYSLFGSTSLPQERIDRHETLLRSLDEDKRMFRRIFDEEAHWLPDELVRFG
                     ALGRKLGDMCITRGNDFLLIDREEAFIRSLAADVENAQRSVHLLFYIFARDAVTEPLF
                     RALEGAAARGVACRVLVDALGAKPFLKRDAERLRAAGIRVEKALPPRLLRRTPTTARY
                     DLRNHRKIAVIDGRVGYTGSHNVTEPSYGGKAKGLLWRDLTLRLVGPVVLQLQGVFME
                     DWFVETREMLLSDEAFPAPEYAGEAVVQTVPSGPSYPWQNYQRLVVSALHGAKKRVVI
                     TTPYLIPDDELLHALETAVLGGVRIQLVIPEKSDQFLVGNAAKAYFDALLEAGVEICL
                     FQDGILHSKTMTVDHDLAFLGTSNFDIRSFALNFELNLVLYGRGETEAIRKAQDVYIA
                     SSRRLTAEEWMERPRAFRVLHGVTKLFSPLL"
     gene            complement(2628..3389)
                     /locus_tag="P5201_02335"
     CDS             complement(2628..3389)
                     /locus_tag="P5201_02335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014806880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HRV97400.1"
                     /translation="MRLVLYNIRYGTGTGWSYHLPFPFSGCLRRTEGRFRRISEYVSK
                     LNPDLVGLVEADSGSFRQNGSCQAKALASRIGGESVFACKYDSGSIVSKTPLLKSQGN
                     AVITKLPMIGTKEHVLSKGVKKTLLEVEFDDFTLFLTHLPLGYAARSVQLGEIAERCI
                     DAKKPVILAGDGNTYRGERELRSFFRKTGFQNANPTNTPTFPSRIPALALDFVLYGPG
                     IEMERFDVPRVRYSDHLPLVCDFTVARPKKERRAQ"
     gene            3601..4995
                     /locus_tag="P5201_02340"
     CDS             3601..4995
                     /locus_tag="P5201_02340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012869394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophanase"
                     /protein_id="HRV97401.1"
                     /translation="MVVKVPPEPFRIKMVEPVRVPGKDEREKALEAARFNMFGLRGAD
                     VYIDLLTDSGTGAMSKQQWAAIMMGDEAYAGADSFFNLKKAVKEVLGFDYTIPVHQGR
                     AAENVVFGSLVKEGDVIPFNMPFDTTRGHIFNCKASSVDCVIDEAFDRTNQHPFKGNV
                     DIAKLEKAIAEYGKARIPFIMVTITNNSGGGQPVSLANLREVSAVAKKHGIPVLLDAA
                     RMAENAWFIKNREKECARMSIAEILKATMDTADAITVSCKKDPLVNIGGLVACRTEEM
                     YYKIVPRVILFEGFATYGGLAGRDLEALAVGLHEMTDEQHLTHRIAQVRYLGDLLEEG
                     DVPCIQPIGGHAVFIDAAAFLPHIPQGSYPADVLSVEIYREGAIRGIGLGALAFETTD
                     EKTGERIFPKLELFRLAVNRRTYTNSHIEYVAESIVNVYKRRDSIRWGLEMVYEPPVK
                     GLKHFLAHLKPVAL"
BASE COUNT         1134 a         1636 c         1589 g          961 t
ORIGIN      
        1 gcgctgtgga aaatatcgtc cgcaacgcgc tccgctacac cccgcagcac acaacagggc
       61 aggacggagg gaaggtgcga gtctccctct tccgcgttcc ggagacagag cggccgcttc
      121 acatcctggt cgaagacgaa ggccccggcg ttcctcccgg cgagctggag actattttca
      181 tccccttccg gcgcgtcgag cgcgcccgcg agcggatatc cgcaagcgaa ggcacggggc
      241 tcggactcgc catcgcacac agggcggcgg cgctgcacgg cggcgttatc agcgcgcgga
      301 accgtccgga gggcgggctc gccgtcgaga tcgagctacc gggaacgtaa acgccgcgcc
      361 ctgaaggaaa ctccgggcgc ggcgttgtat gccaaagagg agtgctccgc gttactgttt
      421 ctgcagctca ctccccggaa cggcggagac atcccctgcg gaggagatga caaagccatc
      481 cccgtcaggc gtccgtgcaa gccgaaggtg cgggttcgac agcaggaagt cctcggggac
      541 gtcgcagtcg ctgaggtatg cggcgcgcat ccttccgtaa atcggcgaat ccttgcttat
      601 gtattcggaa agcagctgaa tcagtacttc cgcaccctgg cagctcccga cgaggatgaa
      661 cggccgcccg aggttgtagt gctccatata gtagtcgaac gccgcgaaga cgtccctccg
      721 gggagcgccg ttcacaagtt cccgccgcct ctcccggggc agcgagctgc ggtaggccga
      781 cgcctggctg tagtaggggg cgtacacatc ccccagcttt tcgaaaaccg gcagctcacg
      841 ctcgaaaagc tccagcgccc cttccgccat gcatggatcg tcgacgccgc gaatcgcagc
      901 ttcttcattc ctgcatgcag tagggtaaag atagaaaata tcggtatcca tcaatgtata
      961 gctcggcaac gagagccagc gcgcaggatc cgaatactcg gcgctttcac cggcggcatt
     1021 ccctgcaaaa gtgaacagca gggcggcaat aacggctgcc cgcaggagat gtattcgctt
     1081 catcacggta cgttgttcct ccttgtttct ttctttaaat ccgctgatcc acggcgaagt
     1141 atatgtcatt tcctcatttc gtcaacaggt tcacgacgac tcggtttaca gcagcggact
     1201 gaagagcttc gtcactccgt ggagcacgcg gaacgcgcgg ggacgctcca tccactcctc
     1261 cgcggtcaga cgcctcgaag aggcgatata gacgtcctgc gccttccgga tagcctccgt
     1321 ctcgcctctt ccgtagagca caaggttcaa ctcgaaattc agcgcaaagg agcggatatc
     1381 gaagttgctc gtcccgagga aggcgaggtc gtggtccacc gtcatagttt tcgagtgcag
     1441 gatgccgtcc tggaagaggc agatctccac tccggcctca agcagcgcgt caaagtacgc
     1501 cttcgcggcg ttccccacga ggaactggtc gcttttctcg gggatgacga gctggatacg
     1561 cacgccgccg agcaccgctg tctccagcgc gtgaagcagt tcgtcgtcgg gaatgaggta
     1621 cggcgtcgtg atgaccacgc gcttcttcgc gccgtggagc gccgagacga caagccgctg
     1681 gtagttctgc cacggatagg acggcccgct cggaaccgtc tggacgaccg cctcgcccgc
     1741 gtattcggga gccggaaacg cctcgtcgga gaggagcatc tcccgcgtct cgacgaacca
     1801 gtcctccatg aagaccccct gaagctgcaa caccacgggg ccgacgagcc ggagcgtaag
     1861 gtcgcgccag agcagcccct tcgcctttcc gccgtaggaa ggctccgtga cgttgtgcga
     1921 cccggtgtac cctacgcggc cgtcgatcac ggcgatcttc cggtggttgc ggaggtcgta
     1981 gcgggcggtg gtcggcgtcc ggcgaagcag ccgaggaggc agcgccttct ccaccctgat
     2041 tcccgctgct cgaaggcgtt cggcgtcgcg cttcaggaat ggcttggccc cgagcgcgtc
     2101 gacgagtacg cggcacgcaa cgccccgcgc cgccgcgcct tcaagcgcgc ggaagagagg
     2161 ctcggttacc gcatcgcggg caaagatgta gaaaagcagg tggacggagc gctgcgcgtt
     2221 ctccacatcg gcggcaagcg accggatgaa ggcctcttcc cggtcgataa ggaggaagtc
     2281 gttcccccgg gtgatacaca tgtcgccgag ctttctgccg agcgcgccga agcgcacaag
     2341 ttcgtccgga agccagtggg cttcttcgtc gaagatgcgg cggaacatcc gtttgtcctc
     2401 gtcgaggctg cgcaaaagtg tttcgtgccg gtcgattcgc tcttggggca gactcgtcga
     2461 gccgaagaga gagtacagca ggaagcccag ccacggccag aagtagatgg cgagcagcca
     2521 tgcggtcgcc gtggaggggg tgtgcttcag cgggatgtag cagagcatga tgaggcggat
     2581 ggcgatcgaa gaaagttcat aggccgaata caagagcatc gtgacggtca ttgagcgcgt
     2641 cgctcctttt tggggcgggc gaccgtgaag tcgcacacca gcggcagatg gtccgaataa
     2701 cggacccgcg gcacgtcgaa acgctccatc tcgatccccg gaccgtagag aacaaagtcg
     2761 agcgcgagcg ccggaatcct gctggggaat gtcggcgtgt tcgtcggatt ggcgttttga
     2821 aaaccggtct tccggaagaa cgagcgcagt tcccgctcac ctctgtaggt attgccgtcg
     2881 ccggccagga taaccggttt cttcgcgtcg atgcagcgct ccgcgatctc gccgagctgc
     2941 acggaccgtg cggcgtaccc caagggaaga tgcgtcagga agagcgtgaa atcgtcgaac
     3001 tcgacttcga gcagcgtctt cttgacaccc ttgctcagca catgctcctt cgtcccgatc
     3061 atggggagct tcgtgatgac ggcgttcccc tgcgatttca gcagcggcgt cttcgaaacg
     3121 atggagccgg agtcgtactt gcatgcgaaa accgactcgc ccccgatccg ggaggccaag
     3181 gccttcgcct gacaggagcc gttctgacgg aacgatccgg aatccgcctc caccagcccg
     3241 acaagatcgg ggttcagttt ggagacatac tccgagatac ggcggaagcg cccctccgtc
     3301 ctccgaagac atcccgagaa aggaaacgga aggtgatagc tccatccggt tccggttcca
     3361 tacctgatgt tgtacaacac taaacgcata tgagcctccc taaacacaga aaatatcgtt
     3421 cattatataa cacctccgcc attcctctac ggtgattcct gctgtttctc caaacctccc
     3481 ggcaggcact gcaaaaagtt tttcaccgat gatgcgaaaa aaatagcaaa acaggagaat
     3541 atagggtaga ataacggaag aaacctgatc ttttctcaat tgaaaaggag gaaaaaattc
     3601 atggtcgtga aggtaccccc cgagcctttt cgcatcaaga tggtagaacc ggtccgcgtt
     3661 cccggcaagg acgagcgcga gaaagcgctt gaagcagccc gtttcaacat gttcggtctc
     3721 cgcggcgcgg acgtctatat cgacctcctc accgactccg gaacaggcgc gatgagcaag
     3781 cagcagtggg ccgccatcat gatgggcgac gaggcctacg ccggcgccga cagcttcttc
     3841 aacttgaaga aggcagtcaa ggaggtgctc ggcttcgact acaccattcc cgtccatcag
     3901 gggcgcgcag ccgaaaacgt cgtcttcggc tcgctcgtca aggagggcga cgtcatcccc
     3961 ttcaacatgc ccttcgacac caccaggggg cacatcttca actgcaaggc gtcctccgtg
     4021 gactgcgtga tcgacgaagc cttcgaccgc accaaccagc acccgttcaa ggggaacgtc
     4081 gacatcgcga agctcgaaaa agccatcgcg gagtacggca aggcgcgcat ccccttcatc
     4141 atggtcacca tcacgaacaa ctcgggcggc ggtcagccgg taagcctggc gaacctcaga
     4201 gaggtctccg ccgtcgcgaa gaaacacggt attcccgtgc tcctcgacgc ggcgcgcatg
     4261 gccgaaaacg catggttcat caagaaccgc gaaaaagagt gcgcgcggat gagcatcgcg
     4321 gagattctga aggccacaat ggacacggcc gacgccatca ccgtctcgtg caagaaggat
     4381 ccgctggtga acatcggcgg cctcgtcgcc tgccgcaccg aggagatgta ctacaagatc
     4441 gtccccagag tcatcctctt cgagggcttc gccacctacg gcggcctcgc gggacgcgat
     4501 ctggaggcgc ttgcggtcgg cctccacgag atgaccgacg aacagcacct cacccaccgc
     4561 atcgcgcagg tccgttacct cggcgatctg ctcgaagagg gcgacgtgcc ctgcattcag
     4621 ccaatcggcg gccatgcggt cttcatcgac gccgcggcct tcctgccgca cattccgcag
     4681 gggagctacc ccgccgacgt gctctcggtc gaaatctacc gcgagggcgc catccgcggc
     4741 atcggcctcg gcgcgctcgc cttcgagacc acggacgaga agacgggcga gcggatcttc
     4801 ccgaagctcg aactcttccg tctcgcggtc aaccgccgca cgtacaccaa cagccacatc
     4861 gagtacgtcg cggagagcat cgtcaacgtg tacaagcggc gcgattcgat ccgctggggg
     4921 ctcgaaatgg tctacgaacc gccggtgaaa ggcctgaaac acttcctggc ccacctgaag
     4981 cccgtcgctt tgtagcgcat ttgaaagaac gggaaccaag agaaaagaag gcggccagcg
     5041 tccgaaacgc gcggccgcct ttttgcatgg gaaaattcat agagtataat aagaaccagg
     5101 ttgacgcaca gccgttttgc ttcgggaaga ccctttattc acaaaaaacg gcctgatttt
     5161 gattggaaag gtggttattt tatgaagtcg acactctata tccgccgctg tcttcttatg
     5221 ttctttgcgg cggcctgtct gttttcacag gcagagggag cggtatacga ggtcaaaccg
     5281 ggaggaaccg gagacggttc ttcatgggaa aaagccctcg
//
LOCUS       DAOTIL010000068         5299 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminobacteriaceae bacterium isolate AMR_MDS_3026
            NODE_18463_length_5299_cov_3.828566, whole genome shotgun sequence.
ACCESSION   DAOTIL010000068 DAOTIL010000000
VERSION     DAOTIL010000068.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868050
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminobacteriaceae bacterium (wastewater metagenome)
  ORGANISM  Aminobacteriaceae bacterium
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae.
REFERENCE   1  (bases 1 to 5299)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5299)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:19:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,224
            CDSs (total)                      :: 3,198
            Genes (coding)                    :: 3,184
            CDSs (with protein)               :: 3,184
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5299
                     /organism="Aminobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18463_length_5299_cov_3.828566"
                     /isolate="AMR_MDS_3026"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:3034609"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(375..728)
                     /locus_tag="P5201_02345"
     CDS             complement(375..728)
                     /locus_tag="P5201_02345"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRV97402.1"
                     /translation="MKKVMLCCLLAVFAVLAVSGSGMAHPPSDLVLSYNRADGMLAVK
                     APHSVPDGKRHYIEEFKVSVDGQKYYELDVEFQIDNKTSLAVFHVGDLKAGAVVEVYA
                     KCNRSGDMTKSLTVE"
     gene            851..2098
                     /locus_tag="P5201_02350"
     CDS             851..2098
                     /locus_tag="P5201_02350"
                     /inference="COORDINATES: protein motif:HMM:TIGR01879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20 family metallo-hydrolase"
                     /protein_id="HRV97403.1"
                     /translation="MNAALLKKWFQTLAPIGALSEGGVTRPAYSAEEDLMFRATADIA
                     RRIGLSVSEDCAGNMFISFPGREKEPCHLIGSHLDSVPGGGRFDGVAGVLAALLVMEK
                     IRRNGEDLPVRAVAFRAEESSLYGLATAGSGVITGSVNIAHLKNARSLSGESLYDAMI
                     RKGYTPGACRLEHALDFMELHIEQGRVLFEAKEPLGVVTAIAAPTRLKVTVLGRQDHS
                     GATPMDLRKDALCAAADIILATEAAGRDEMRFATVATVGVANVVPNALNVVPGSVELR
                     IDIRGIQRESIRRTVGKVQRAIEEIAASRGLSCATETISSMDPVTLAPSMIDSLAEAA
                     SGTGVPFRKMASGAGHDAMKVAPVVPTGMLFIPCDEGISHNSAENASLNDIALGAEVM
                     LRSISLRSKHLSTADRSQPFSKL"
     gene            2638..2850
                     /locus_tag="P5201_02355"
     CDS             2638..2850
                     /locus_tag="P5201_02355"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRV97404.1"
                     /translation="MQNTRLNEASPVLSVLAVQGGRVTGKTAKERPKRDGRKRGFDDY
                     LRKAGYESRFDQELEDRLAESIDTLA"
     gene            complement(2966..4618)
                     /gene="pgm"
                     /locus_tag="P5201_02360"
     CDS             complement(2966..4618)
                     /gene="pgm"
                     /locus_tag="P5201_02360"
                     /EC_number="5.4.2.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR01132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004614 - phosphoglucomutase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglucomutase
                     (alpha-D-glucose-1,6-bisphosphate-dependent)"
                     /protein_id="HRV97405.1"
                     /translation="MSVSPMAGERPDHTMLANISALVASYYTVRPDTDDARSLVAFGT
                     SGHRGSSLKGSFNEAHIIAVSAAIVEYRKSAGITGPLFVGKDTHALSEPAFRTAVEVF
                     AAAGVRTVIQKGHGYTPTPVISRAILQWNKGRSEGLADGVVITPSHNPPEDGGFKYNP
                     PSGGPADPEMTDAIQKRANELLHSGTDAVRRIPFEKALGAPCVLEEDYVLPYVDDLAS
                     VLDMEAIARSGIRIGADPMGGSGVAYWSVIAERYGLDLEVVNPSVDPTFSFMSVDWDG
                     KIRMDCSSPYAMAGLVELKDRYDIAWGNDSDFDRHGIVVPDRGLLPPNAYLAVAVEYL
                     FTHRPGWGSDAAVGKTLVSSSLIDRVAHSVGRKCVEVPVGFKWFVEGLLNGSMGFGGE
                     ESAGASFLRRDGTVWSTDKDGIILNLLAAEMLAVTGEHPGKRYDAITARLGEPFYARI
                     DAPASTERKKLLKTLSPEAVKASTLAGEPIVARLTAAPGNGAPIGGLKVTSENGWFAA
                     RPSGTEDVYKIYAESFLGAEHLTRIQSEAREIVDGVFAAAGV"
     gene            4965..>5299
                     /locus_tag="P5201_02365"
     CDS             4965..>5299
                     /locus_tag="P5201_02365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013047488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HRV97406.1"
                     /translation="MFSWKRIRYGVMFAFLAYISWVGYQHQVLGGGPAGIPTVDALCP
                     LGGLESIYSYLTSGVWLRRVAPSALILFIAVAGITLLLGRVFCGWICPLGTIGEFSNL
                     AAKKLGIRQR"
BASE COUNT         1097 a         1620 c         1496 g         1086 t
ORIGIN      
        1 cgtcttcccg gccccgttcg gaccgagaaa tccgaagagc tcgccttcgt agacgtcgaa
       61 atcgattccg cgaaccgcct ccacttcccc aaaacgtttc accagcccgc gcgcctcgat
      121 tgccgctcct cttccctttt ccgttccgat ctccgctccg tccattgcac ccctcttctc
      181 caggtctccc ccaaagactc ccgcagccgc cgaaaaagca agaaactcct cgacgggagc
      241 ggggccggcg ggttcagcgc acgaaccgcc ccggatatac tattttcttt ccatttcaat
      301 gtcaagaaaa agacgcccct cgcgggacgt cttctgtggt ctgtttcttc cgtttactgc
      361 gatgctcggc gcgcttactc caccgtcagg gatttcgtca tatcccctga tcggttgcac
      421 ttcgcataca cctcgacgac cgccccggcc ttcaaatcgc cgacgtgaaa gacggcaaga
      481 gaggtcttgt tgtcgatctg aaactcgacg tcgagctcgt aatacttctg cccgtctacc
      541 gagaccttga attcttcgat ataatgtctt tttccgtcgg gtacggaatg cggcgccttc
      601 acggcaagca ttccgtcggc gcgattgtag gacaacacca ggtcggaagg cggatgcgcc
      661 attcctgagc cggagacggc gagcaccgca aatactgcga gaagacagca gagcatgacc
      721 tttttcattg tacctcactc tcccttttct gtacgatttg aactgctaca acatggtaat
      781 tttatcacac acgcagcgca cgaagcaaat gtattgagaa gattcggctg caatacaacg
      841 aggtgatcga atgaacgcgg cactcctgaa aaaatggttt cagacgctcg cacccatcgg
      901 cgcgctctcc gaaggaggcg tcacccggcc ggcctactcg gccgaagagg atctcatgtt
      961 ccgcgctaca gcagacatcg cccggcggat aggactctcc gtcagcgagg actgcgcggg
     1021 aaatatgttc atctcatttc cgggacgcga aaaagaaccg tgccacctga tcggctcgca
     1081 cctggactcg gttcccggcg gcgggcgttt cgacggcgtg gcgggcgtac tcgccgcgct
     1141 cctcgtgatg gaaaagatcc ggagaaacgg cgaagatctt cctgtgcgcg ccgtcgcctt
     1201 ccgggccgag gagtcgagcc tctacggact tgccacggcg ggaagcggcg tcatcactgg
     1261 aagcgtgaat atcgcgcact tgaagaatgc ccggtcgctt tccggagaga gtctgtacga
     1321 cgcaatgata cggaaggggt acacgccggg agcgtgcaga ctggagcatg cgctcgactt
     1381 catggagctg catatcgagc agggccgggt gctcttcgag gcaaaagaac cgctgggcgt
     1441 cgtcacggcg atcgccgcac ccacgcgcct caaggtaacg gttctcggac gccaggatca
     1501 ctccggagca accccgatgg acctgcgtaa ggacgcgctc tgcgcggctg ccgatattat
     1561 tctcgcgacc gaagcggcag ggcgtgacga gatgcgcttc gctaccgtcg caaccgtggg
     1621 cgttgcgaac gtcgtcccga acgcgctcaa cgtcgtcccc gggagcgtgg aactccggat
     1681 cgacatcagg ggaatacagc gcgagagcat caggagaacg gtcgggaaag tccagcgcgc
     1741 catcgaggag attgcagctt cgcgaggtct ttcgtgtgcg acggagacga tctcctccat
     1801 ggatccggtg acgctcgctc cctcaatgat cgattcgctc gccgaggcag cttccgggac
     1861 aggcgttccc ttccggaaga tggcgagcgg cgcggggcat gacgcaatga aggtggcccc
     1921 cgtcgtaccg acgggcatgc tctttatccc ctgcgacgag gggatcagcc acaactccgc
     1981 agaaaacgcc tcgctcaacg atattgcgct cggcgcagag gttatgctcc gaagcatctc
     2041 cctcaggagc aaacatcttt ctactgccga tcgctctcaa ccgttttcga agctgtagag
     2101 gatatcgaaa accgagcgtc gcgggcaatt tttcccaata cactacaagt acataaataa
     2161 agattactat ctgaagagca aaaaaagtaa attgatgaaa tttatcattt tgacttgaat
     2221 taaactgcct attatggtag tttaagaacg aaacatcgat gcagttctcc gactctcttt
     2281 cacttttttc agcctcgttt ttccgcgcgg gttttctgag gtgtatttga gccacagctc
     2341 ttccaaagtt ttgaaaaagg aggtgttatc tatgaacacc gccactgtca tctggcttgg
     2401 gatcatggct gttatcggcg tcgtcatgct tgtagtaaaa aaacgctctg caagcagaaa
     2461 caacgaggaa gcgtagtcat tcttcctctc tactctacag attttttcca catcgttttt
     2521 ttctgcacgc gtaggaaggc ggctcatgag agccgccttc cgtgttatcc ccctcctgat
     2581 ccggaattaa caaactcttc gcgcactacc gatacattgt tataaggggg gagcgccatg
     2641 cagaacacgc ggctgaacga ggcgtctcca gtcctttcag ttctcgcagt ccaaggaggg
     2701 cgggtcactg gaaagacggc gaaggagcgc ccgaaaagag acggaagaaa acgcggtttt
     2761 gacgactatc tccgcaaagc cgggtatgag tcccgctttg accaggagct ggaagatcgt
     2821 ctcgccgaaa gcatcgacac tcttgcataa acaacggaac agcgaacaga ggaagacgtc
     2881 tccggaagag ttcggaacat agaaaaagaa cagcggcctc cgcgtccgat cgtgcgcgga
     2941 ggccgcttgc tgaaaaatcc gagtatcaga ctccggccgc cgcgaacact ccgtccacga
     3001 tctcgcgcgc ttcgctctgg atacgggtaa gatgctccgc ccccaggaag ctctccgcat
     3061 agattttgta cacgtcctcg gtccccgacg gccgagccgc gaaccacccg ttttcggacg
     3121 tcactttcag gccgccgatg ggcgccccgt ttccgggggc cgctgtcaac cgggcaacga
     3181 tcggctctcc ggcgagcgtc gacgctttca ccgcctcggg agagagcgtc ttcagcagct
     3241 tcttccgctc cgtcgacgca ggcgcgtcga tgcgcgcgta aaaaggctct ccaaggcggg
     3301 ccgtgatggc gtcgtaacgt ttcccgggat gctctccggt gactgcgagc atctccgccg
     3361 caagcagatt gaggataata ccgtccttgt cggtactcca gaccgtcccg tccctgcgaa
     3421 ggaaggacgc ccccgcgctc tcctcgccgc cgaatcccat cgatccgttc agaagccctt
     3481 cgacgaacca cttgaacccg accggaacct cgacgcactt ccttccgacg ctgtgcgcaa
     3541 ccctgtcgat cagcgaactg gagacgagcg tcttcccgac ggcggcgtcc gaaccccacc
     3601 ccggacggtg cgtgaagaga tactccacgg cgacggcaag gtacgcgttc ggcggaagca
     3661 gccccctgtc aggaacaacg atgccgtgcc tgtcgaaatc cgagtcgttt ccccaggcga
     3721 tgtcgtacct gtccttcagc tccaccagtc cggccatcgc gtagggagag gagcagtcca
     3781 tgcggatctt gccgtcccag tccacggaca tgaaggaaaa tgtcgggtcg accgaggggt
     3841 tcaccacttc gaggtcgagc ccgtaccgtt cggcgatcac gctccagtat gccacgccgg
     3901 agccgcccat cgggtcggcg ccgatgcgaa tcccggaccg ggcgatggcc tccatgtcga
     3961 ggacggaggc gaggtcgtcc acgtacggca gaacatagtc ctcttcaagg acgcacggag
     4021 cgccgagcgc cttctcgaac gggatgcgcc ggaccgcgtc cgtgccggag tgaagcagct
     4081 cgttggctcg cttctggatt gcgtcggtca tctcggggtc cgccggtccg cccgacgggg
     4141 ggttgtactt gaagcccccg tcctccggcg ggttgtgcga cggagtgatg acgacgccgt
     4201 cggcgagtcc ctcgcttctc cccttgttcc attggagaat ggcgcgcgaa attaccggcg
     4261 tcggcgtgta tccgtgtcct ttctggatca ccgtccggac gccggcggcg gcaaaaactt
     4321 ccacagccgt cctgaacgcc ggctcggaga gggcgtgcgt atccttgccg acgaagagcg
     4381 gccctgtaat ccccgcgctc ttccggtact cgacgatcgc cgcgctgacc gcaataatgt
     4441 gcgcctcgtt gaaggaacct ttcagggaag atcccctgtg ccccgacgtt ccgaaggcta
     4501 cgagcgagcg cgcatcgtcc gtgtctggac ggactgtgta gtaggatgcg accagcgcgg
     4561 atatgtttgc caacatcgta tggtccgggc gttcgccggc catcggcgat acgctcatcg
     4621 tgattccccc ctgttcatca acatacatgt cggcccggga cgggttctcg acacggccgg
     4681 aagtcttccg cgcgcagccg ctgccgcacg ggataacgat gcaacaatac caggagattc
     4741 aaaggatatt atactagaaa attctctttc attcacttcc gtacctgccc gaatccgcag
     4801 gcgaagaggg gattgcactg cgcgcattct gtgcatgagc ctacggatgc tcctctctgc
     4861 tctccgtaat atgacgtacc cccggagtgt ataaagaaac aatagtattc cagtccggat
     4921 ggtgctattc tattagaaaa ttctcatcga gggggaggat ctgcatgttt tcatggaagc
     4981 gtatccggta cggcgtcatg ttcgcttttc tggcgtacat cagttgggtg gggtatcagc
     5041 atcaggtatt gggaggagga ccggcgggaa taccgaccgt cgacgccctc tgtcctctgg
     5101 gaggccttga aagcatctac agctacctta cttcgggcgt ctggctccgc cgcgtcgcac
     5161 ccagcgcgct gatcctcttc atcgccgtcg cgggcatcac gctgctgctc ggcagggtct
     5221 tctgcgggtg gatctgcccc ctggggacca tcggagaatt cagtaacctc gcggccaaaa
     5281 agctcgggat acggcagcg
//
LOCUS       DAOTIL010000069         5286 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminobacteriaceae bacterium isolate AMR_MDS_3026
            NODE_18517_length_5286_cov_4.050660, whole genome shotgun sequence.
ACCESSION   DAOTIL010000069 DAOTIL010000000
VERSION     DAOTIL010000069.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868050
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminobacteriaceae bacterium (wastewater metagenome)
  ORGANISM  Aminobacteriaceae bacterium
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae.
REFERENCE   1  (bases 1 to 5286)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5286)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:19:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,224
            CDSs (total)                      :: 3,198
            Genes (coding)                    :: 3,184
            CDSs (with protein)               :: 3,184
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5286
                     /organism="Aminobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18517_length_5286_cov_4.050660"
                     /isolate="AMR_MDS_3026"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:3034609"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1082)
                     /locus_tag="P5201_02370"
     CDS             complement(<1..1082)
                     /locus_tag="P5201_02370"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014163802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HRV97407.1"
                     /translation="MAKKRYQHELELRQFAVDDYDEVKRIMDRIYPKMGGAWTKEQFS
                     SMISRFPEGQLCIEDKGRVVAGALSLIIDYSRYGDRHTYRDIVGDGYLKNHDPEGDYL
                     YGIDIFVSPDYREKRLGRRLYDARKEQCETLNLKGIIIGGRIPGYAEWAEKKTPQQYI
                     RMVNNREISDPVLTFQVSNDFHYKKIVENYWPEDYSSKGYGVILEWNNIYYEERQRLI
                     SKVKTYMRLGVVQWQMRSFESFEDFLQQVEFFVDTQAGYNTDLMLFPELFNAPLLAQF
                     DQEDPAEAMRLLAGYTADLRRNLVEMAVAYNINIVSGSVPELRNNRLYNVTFLCRRDG
                     TWDQQYKLHITPDEYRCWGLQGGHDL"
     gene            1360..2619
                     /locus_tag="P5201_02375"
     CDS             1360..2619
                     /locus_tag="P5201_02375"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HRV97408.1"
                     /translation="MERCGGVHFEKVVNKSPVTSFIWLPSENGKPCFVSEAIRSFGYA
                     PEDFTSGKIRYADLLHPDDVAAFSFSPGSEPQIYRISDSEGAWRWVFHRSCLIGEEAG
                     YPEELLWNISDITYYKEADEELRKNESKLKALMKAIPAILLVIDEDGRYIDVSGSSEA
                     ALLTASSELIGKTVHESFPNEKAEELMAPLKRCVETGAPQFAAYDIDIGGKKYFQEAR
                     ISLLPELVEGKRTVICVALDVTAQKELEEEILLQTGHNPIDGSSNRTHFNRAFLFSLA
                     EAQRTGGTLAFFMIDLDHFNTVNETIGREMADLLLKGIGQRLQKVCRQDDILYRMEGV
                     NYYLILPKPGTREDMGAVAERFLETIRGASSGYTGSFKLSATIGISVFPEDGQDAKTL
                     LRTAEAALSAGKAEGGNCYRFAEKPAR"
     gene            complement(2699..3964)
                     /locus_tag="P5201_02380"
     CDS             complement(2699..3964)
                     /locus_tag="P5201_02380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009200651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerate kinase"
                     /protein_id="HRV97409.1"
                     /translation="MTDVRQDALHIAQKAIEAVLPENAVEAALRGEAFSARAQSGGRI
                     VLTAIGKAAWRMAKAAAETLGDRLDGGVVVTKYGHSNGPIKGVEIFEAGHPFPDENTI
                     KGTARALERVGGLSAEDTVLFLVSGGGSALFEKPGEGITLRDLVGVTDQLLACGADIV
                     EINTIRKRLSSVKGGRFAQFVAPAHVFSVVLSDVLGDRLDSIASGPAYPDGSTAADAS
                     RIVGKYGLSLSPVLQKALEKETPKSLENVTTLITGSVTALCKAAEREVAALGYTPLLL
                     TTTLNCEAREAGAFIAAMAKEIRSSGNPASPPCALLLGGETIVRLKGKGKGGRNQEMA
                     LAAAFGIKGLADTAVISVGSDGTDGPTDAAGGVVDGGTIGVLSGLGLDAEALLADNDS
                     YHALDACGGLVKTGPTGTNVNDLSILLCR"
     gene            complement(4065..>5286)
                     /gene="larA"
                     /locus_tag="P5201_02385"
     CDS             complement(4065..>5286)
                     /gene="larA"
                     /locus_tag="P5201_02385"
                     /inference="COORDINATES: protein motif:HMM:NF033504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050043 - lactate racemase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="nickel-dependent lactate racemase"
                     /protein_id="HRV97410.1"
                     /translation="KAVLVSSLHELKADAPEREIVRKSLDNPIGSPRLRDLAKGKENV
                     VIISSDHTRPVPSAIIMPLLLEEVRAGNPDAQITILVSTGGHRATTPEELLKKYGPEI
                     VKNERIVVHDSHDDTSLVHVGTLPSGGDLILNKLAVEADLLVAEGFIEPHFFAGFSGG
                     RKSVLPGVASYTTVMANHCAEFIAHERARTGILEGNPIHIDMVFAARKVGLAFVCNVV
                     IDAEKHIVAAFSGDMEAAHEKGCEFVAKYGRVKAVPADIVVTSNAGYPLDQNLYQAVK
                     SMTAGEASCTKGGVIIVAAECSDGHGGEAFYRTFETIPTPKGIMDMIMARGRNQTEPD
                     QWQIQIFARVLMDFTVIMVTSAPKEMAEHLNMQWAPSLEEALSMAERILGKPDASVTV
                     IPDGVAVIVDAFSS"
BASE COUNT         1044 a         1651 c         1498 g         1093 t
ORIGIN      
        1 ttaaggtcgt gccccccctg caacccccag cagcggtact cgtcgggtgt gatgtggagc
       61 ttgtactgct ggtcccatgt gccgtcgcgg cggcaaagaa aagtgacgtt gtagagcctg
      121 ttgttccgga gctccggcac gcttcccgag acgatgttga tgttgtaggc caccgccatc
      181 tccacgagat tcctgcgaag atcggccgta taccctgcga gcaggcgcat ggcctccgcg
      241 ggatcttcct ggtcgaactg cgcgagcagc ggcgcgttga acagctcggg gaagagcata
      301 aggtcggtgt tgtagccggc ctgggtatcc acaaaaaact ccacctgctg cagaaaatcc
      361 tcgaaggact cgaagctccg catctgccac tggaccacgc cgaggcgcat gtaggtcttc
      421 actttggaaa tgaggcgctg ccgctcctcg tagtagatat tgttccattc gagaataacg
      481 ccgtacccct tcgagctgta gtcctccggc cagtagttct ccacgatctt cttgtagtgg
      541 aagtcgttgc tcacctggaa cgtcagcacc ggatcggaga tctcccggtt gttcaccata
      601 cggatatact gctgcggcgt tttcttctcc gcccactcag cgtacccggg gattctgccg
      661 ccgatgatga tccccttcag attgagcgtc tcgcactgct ccttccgcgc gtcgtaaaga
      721 cgtcggccca ggcgtttctc tctatagtcg ggagagacga agatatcgat tccgtagagg
      781 tagtctcctt ccggatcatg gtttttcagg tatccgtcac cgacgatatc cctgtacgta
      841 tgccggtcgc cgtagcggga gtagtcgatg atgagagaga gcgcgcctgc aaccacccgt
      901 cctttgtcct ctatgcagag ctgcccctcg ggaaagcgag agatcatgga agaaaactgt
      961 tcttttgtcc aggcgccccc catcttgggg tatatcctgt ccatgatgcg cttgacttcg
     1021 tcgtaatcat caacggcgaa ctggcggagc tcaagctcat gctggtagcg tttttttgcc
     1081 atcggctctt cattcctttc aacttccgta ttctgctatt atacgttgac gacatgcaaa
     1141 aaacacccgc aaacggagga agcgaacccg ggctcttcag ttttttccgt gcatctttct
     1201 cttttccgga aatcagcccc caacgacaca acacgttgaa aacgccgcac atccgggaaa
     1261 aactgtttcg gcaggttcat atatgctaca ataccttgag gaaaagaaaa acattccgga
     1321 ttctccggga catacctcta aagggagatg aaagccggca tggagcgatg cggcggagta
     1381 cacttcgaaa aggttgtcaa caagagtccc gtgacttctt tcatctggct cccctccgaa
     1441 aacgggaaac cgtgttttgt atcggaagcc atacgctcgt tcggctacgc gccggaggac
     1501 ttcacatccg ggaaaatcag atacgccgat cttcttcacc cggacgatgt cgccgctttc
     1561 tcattctcgc ccggctccga accccaaatc tacaggatct ccgactcgga aggggcgtgg
     1621 cgctgggtct ttcaccggtc gtgcctcatc ggcgaagaag cgggctaccc ggaggagctc
     1681 ttgtggaata tctcggatat cacctattac aaagaggccg acgaagagct gcggaaaaat
     1741 gaatcgaagc tcaaggcgct tatgaaggca atcccggcga ttctcctcgt cattgacgaa
     1801 gacggacggt acatcgacgt ctcgggttcc tccgaagcgg ctctcctcac ggcgtcctcg
     1861 gagctgatcg gaaaaaccgt ccacgagtcg ttcccgaatg agaaagcgga ggagctgatg
     1921 gcgccgctga aacgctgcgt cgagaccggc gccccgcagt tcgcagccta tgacatcgac
     1981 atcggcggga aaaaatattt tcaggaagcc cgcatctccc tcctgccgga gcttgtggag
     2041 ggcaaacgga ccgtgatctg cgtcgccctc gacgtcaccg cgcagaaaga gcttgaggag
     2101 gagatcctcc tccaaaccgg acacaacccg atcgacggaa gctcgaacag gacgcatttc
     2161 aacagggcgt tccttttctc cctcgccgag gcgcagcgaa cgggcggaac gctggctttc
     2221 ttcatgatcg atctcgacca tttcaacact gtaaacgaga ccatcgggag agagatggcc
     2281 gaccttctac tcaagggtat agggcagcgg cttcagaagg tgtgccggca ggacgacatt
     2341 ctctaccgca tggagggcgt caactactac ctcatactgc cgaagccggg aaccagggaa
     2401 gacatggggg ccgttgcgga gcgcttcctg gaaacaatca ggggagcgtc ctcgggatac
     2461 accggctcat tcaagctttc cgccaccatt ggcatcagcg tcttcccgga agacgggcag
     2521 gacgcgaaaa cgctgctcag aacggcggaa gcggctcttt ccgcaggaaa agcggagggc
     2581 ggcaactgct acagattcgc tgagaaaccc gccaggtaat ttttctccgc cactgcacat
     2641 gaatatgacg cgaggcccct tttcaggggc ctcgttctgt ctgcacccga aagagcgact
     2701 acctgcagag gagaatggag aggtcgttca cgttcgttcc ggtgggcccc gttttgacaa
     2761 ggcctccgca ggcatcgagc gcatgatagg agtcgttgtc ggcaagcaga gcttcggcat
     2821 caagtccgag accggaaagc acgccgatcg tcccgccgtc caccacgcca cccgccgcgt
     2881 ccgtcggccc gtccgtgccg tcggaaccga cggaaatcac cgccgtatcc gcaagcccct
     2941 tgatgccgaa ggccgcagcc agagccatct cctggttccg tccgcccttt cccttcccct
     3001 tgagccggac gatcgtctcc ccgccgagga gcagggcgca cggaggcgac gccgggtttc
     3061 cggaagaccg gatctccttc gccatagcgg cgatgaaggc ccccgcttcg cgggcctcgc
     3121 agttcagcgt cgtcgtcagc aggagcggcg tatagccgag tgcggcgacc tcgcgctccg
     3181 ctgctttgca gagcgccgtc acgctcccgg taatgagcgt ggtcacgttt tcaagcgatt
     3241 tcggggtctc cttctccaaa gctttctgaa gcacagggct caacgacagc ccgtacttcc
     3301 cgacaatcct cgacgcgtcg gcggccgtcg aaccgtccgg ataggccggg ccggaggcga
     3361 tgctgtcgag tctgtcgccg agaacgtcgg agagcaccac cgaaaagacg tgcgccggag
     3421 cgacgaactg agcgaaccgc ccccccttga ccgaggagag ccgctttcgt atcgtgttga
     3481 tctcgacgat gtcggcgccg catgcgagca gctggtcggt cactccgacg agatcccgga
     3541 gggtaatccc ctcgcccggc ttctcgaaga gggccgagcc gccgccggag acgaggaaga
     3601 gcacagtatc ctccgcggag agtccgccga ccctttcgag cgcacgggcc gtgcccttga
     3661 tcgtgttctc gtccggaaac gggtgccccg cttcgaaaat ctcgacgccc tttatcgggc
     3721 cgttgctgtg tccgtacttc gttacgacga cgccgccatc gagccggtct ccaagcgtct
     3781 ccgcagcggc cttcgccatc cgccacgccg ctttgccgat ggcagtgagc acgatcctcc
     3841 ccccggactg cgcacgcgct gaaaaagcct ctccgcgcag cgccgcctcc acggcgtttt
     3901 ccgggagcac ggcctctatg gccttctgcg caatgtgtaa ggcatcctgc cgaacatccg
     3961 tcattccttc tcctcctcag tttttatttc gccgccagca cggagaaaca ctccgccatc
     4021 gtccgttcgc cgccgaacga gcccgttcgc gcatccctgt tttttcagga agagaacgca
     4081 tcgacgatca ctgccacgcc gtccgggatg acggtgaccg atgcgtccgg ttttccgagg
     4141 attcgctccg ccatcgacaa ggcctcctcc agggacgggg cccactgcat gttgagatgc
     4201 tccgccatct ccttgggggc cgaggtcacc atgatcacgg tgaaatccat cagcactctg
     4261 gcgaatatct ggatctgcca ctgatccggc tccgtctgat ttcttcccct cgccatgatc
     4321 atatccatga tccccttcgg cgtcggaatc gtctcgaagg tccggtagaa ggcttcgccg
     4381 ccgtgaccgt cgctgcattc ggcggcgacg atgatgacgc cccccttggt gcacgacgcc
     4441 tcccccgcgg tcatgctctt caccgcctga tagaggttct gatccagggg ataccccgcg
     4501 ttgctcgtga cgacaatgtc ggcgggaacg gccttgaccc tgccgtactt ggcgacgaac
     4561 tcgcacccct tctcgtgcgc cgcttccatg tcgccgctga aggccgccac gatatgcttc
     4621 tccgcgtcga tgaccacgtt gcagacgaac gccagcccga ccttgcgggc agcaaagacc
     4681 atatcgatgt ggatggggtt gccctcgagg attccggttc tggccctttc gtgcgcgata
     4741 aactccgcgc agtggttcgc catcaccgtt gtgtagctcg ccacgccggg gagtacgctc
     4801 ttgcggccac ctgagaaacc ggcgaagaag tgcggctcaa tgaacccctc cgcgacaagc
     4861 aggtccgcct cgactgccag tttattgagt atcaaatcgc ctccggaagg aagggtccct
     4921 acgtgaacga ggctcgtatc gtcatggctg tcgtggacca caatgcgttc gttcttcacg
     4981 atctcgggac cgtacttctt caggagctcc tccggggtgg tagcccggtg gcctccggta
     5041 gagaccagga tagtgatttg tgcatcgggg tttcccgccc gcacctcttc gagaaggaga
     5101 ggcatgataa tggcgcttgg gacggggcgc gtatggtcgc ttgagatgat cacgacgttc
     5161 tctttcccct tcgcgaggtc tcgcagacga ggggatccga tcgggttgtc gagggatttt
     5221 ctgacgattt cgcgctccgg cgcatcggct ttcagttcgt gaagactcga aacgagaacc
     5281 gctttc
//
LOCUS       DAOTIL010000070         5280 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminobacteriaceae bacterium isolate AMR_MDS_3026
            NODE_18550_length_5280_cov_3.999426, whole genome shotgun sequence.
ACCESSION   DAOTIL010000070 DAOTIL010000000
VERSION     DAOTIL010000070.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868050
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminobacteriaceae bacterium (wastewater metagenome)
  ORGANISM  Aminobacteriaceae bacterium
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae.
REFERENCE   1  (bases 1 to 5280)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5280)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:19:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,224
            CDSs (total)                      :: 3,198
            Genes (coding)                    :: 3,184
            CDSs (with protein)               :: 3,184
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5280
                     /organism="Aminobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18550_length_5280_cov_3.999426"
                     /isolate="AMR_MDS_3026"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:3034609"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..199)
                     /locus_tag="P5201_02390"
     CDS             complement(<1..199)
                     /locus_tag="P5201_02390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008521688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRV97411.1"
                     /translation="MLEMLKKALGLDPNERALKRYTSVVEQINALEPALLSLSDEELR
                     EKGASFNNPAMSSFEKPLSDNL"
     gene            complement(340..909)
                     /gene="pgsA"
                     /locus_tag="P5201_02395"
     CDS             complement(340..909)
                     /gene="pgsA"
                     /locus_tag="P5201_02395"
                     /EC_number="2.7.8.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006300641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008444 -
                     CDP-diacylglycerol-glycerol-3-phosphate
                     3-phosphatidyltransferase activity [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol--glycerol-3-phosphate
                     3-phosphatidyltransferase"
                     /protein_id="HRV97412.1"
                     /translation="MGKKVWNIPNTLSLLRVFLAPLVLIFLTFKFTYPVYSVEDLGIL
                     VTVGDVLAGSVFILAALTDTADGYIARKKGMVTNLGKFIDPLADKILVMAALIALVEL
                     HRIPAWIVVVILTREMIVTGVRMVAAAEGIVIAASNAGKLKTVFQIVAIAMIIFNIPF
                     GIHVMWVAMLMTLWSGMDYLIKGKKLFTE"
     gene            complement(1019..1747)
                     /locus_tag="P5201_02400"
     CDS             complement(1019..1747)
                     /locus_tag="P5201_02400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012869391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HRV97413.1"
                     /translation="MLKIENLHVYYGAIHAVKGINLEIPEGAIVTLIGANGAGKSSTI
                     RAVAGLVKNVKGKILYTPSGGDASGQVNLIGKTPEEMVKLGIAMSPEGRRILPHLTVE
                     ENLQLGAFIRNDKDGIERDRDHVYELFPRLKERSWQKGGTLSGGEQQMLAVGRALMSQ
                     PKLVMLDEPSLGLAPLLVKEVFEIIGEINALGKTVLLVEQNAFAALKIASYAYILEVG
                     NIVLEGKGEELLKDARVKEAYLGG"
     gene            complement(1734..2528)
                     /locus_tag="P5201_02405"
     CDS             complement(1734..2528)
                     /locus_tag="P5201_02405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013048810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HRV97414.1"
                     /translation="MTTPASILRTENLTMRFGGLTAVGNFSIDVPQGKIVGLIGPNGA
                     GKTTVFNMITGFYRPTEGRIFFDGQDITGFQPHMVCRSGISRTFQNIRLFTNETVLEN
                     VMIGCHVRRKSKWWMAPLGLPSFRAEEEAIREKSMDLLKSVGLEDFADEPSASLSYGA
                     QRRLEIARALATDPKFLLLDEPAAGMNPQESQELMSFIRNIQKRFNLTILLIEHDMKV
                     VMGVCEYIWVLDYGVKIAEGNPDAIQSDQKVIVAYLGEEYVKYVEN"
     gene            complement(2525..3592)
                     /locus_tag="P5201_02410"
     CDS             complement(2525..3592)
                     /locus_tag="P5201_02410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013048811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HRV97415.1"
                     /translation="MNTRTRNFILNCLLIAALGAFLYWAQNTLDGYKIQVLNLIAVNA
                     ILALSLNLIYGFTGMFSLGHAGFMAIGAYVCSILILSPAQKEAMWILEPLIWPLSVIK
                     APFFVAVLSGGLIAAFFALLIAIPVLRLGGDYLGIATLGFAEIIRVVLTNLSFITNGA
                     LGIKGIPPYANIWWNYAWLLLTLFFIIRLVNSNFGNVLKAIRDDEIAAKNMGINTFFY
                     RVLAFTIGAFFAGVGGALMGSLITTIDPRMFMFVLTFNILMIAVAGGLGSITGSIIGS
                     IVITILLEWLRFVENPLVIGSFEIPGIPGMRMVIFSLALILIILFRREGIMGMREITW
                     DTFFSRGRRSPEHEEKGGKMK"
     gene            complement(3592..4485)
                     /locus_tag="P5201_02415"
     CDS             complement(3592..4485)
                     /locus_tag="P5201_02415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006583780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0015658 - branched-chain amino acid
                     transmembrane transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HRV97416.1"
                     /translation="MSGSMFIQHLFNAITLGSLYGLIAIGYTMVYGILRLINFAHGEI
                     FMLGAYLVFYAITLLNLPWGLAVVVAILGSSLCGIFVDKVAYKPLRDAPRISALISAI
                     GVSFLIQNLSIVIFTGIPRPVARPDWLVKVFKIGNVRILPLAIVVPIVTVLLVLGLLW
                     IVYRTKPGLAMRAISKDIETTRLMGVSVNKIIAFTFGIGSALAAASGIMWALRYPQIH
                     PFMGLVPGLKAFIAAVFGGIGSIQGAMIGGMLLGLIEIMAVAFFPHLSGYRDAFAFVL
                     LIVILLLKPTGLMGEKLEDKI"
     gene            complement(4608..>5280)
                     /locus_tag="P5201_02420"
     CDS             complement(4608..>5280)
                     /locus_tag="P5201_02420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015556964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HRV97417.1"
                     /translation="SLGMKKAALLIDVANDYCVGLANFFKQSYKKLGGEIVSELKYNS
                     GDQDFTAQLTEIISQNPDLLFIPADFAEGAIIMKQARELGAEFQIMGGDAMDNPEIVN
                     IGGEAVEGFIHTTFPYDPSMTDMSPIAKTFTENWTKNNPDKAPNVNAALGYDSYMLVV
                     DAIKRAGSAEPDAIRTALSSTKDFPGVTGSKTINATHDAESPVGIVQIKDGKKQFIGT
                     VEPQL"
BASE COUNT         1330 a         1405 c         1378 g         1167 t
ORIGIN      
        1 ataaattatc cgaaaggggt ttttcgaatg agctcattgc cgggttgtta aatgaagcgc
       61 ctttctcgcg caactcttcg tcggagagcg ataaaagggc aggctcaaga gcattgatct
      121 gttctacgac acttgtatag cgcttcaggg cccgttcgtt aggatccaga ccgagggcct
      181 ttttgagcat ttcgagcata gcgtcacctc aacaaaaaat tcggccgtgc agcgcgcacg
      241 gcacataata ccgggtcatt atatgtcccg aacccgtcgt ggtcaaagta tttatgcgga
      301 cctcccggaa acggcgcgtc aggactgaaa aacgcacggc tactcggtaa agagtttttt
      361 cccctttatc agatagtcca tccccgacca gagcgtcatc agcatagcca cccacataac
      421 atggattccg aagggaatgt tgaagatgat catcgcaatt gccacgatct gaaagaccgt
      481 cttcagtttg cccgcgttgg aggccgcaat gacaatgcct tcagctgcgg cgaccatcct
      541 gactcccgtg acgatcatct cccgggtgag aatgacaacc actatccagg ccgggatacg
      601 gtgaagctcg accaaagcta taagagccgc catgacaaga atcttgtccg cgagcggatc
      661 aataaacttg ccaaggtttg taaccattcc tttcttccgg gcgatatagc cgtcggccgt
      721 atccgtcagg gctgcgagaa taaacacgga gccggccaaa acatcgccga ccgtcaccaa
      781 aatcccgaga tcttcgacac tgtagacagg gtacgtaaat ttgaacgtca ggaaaatcaa
      841 aacgagaggc gcgagaaaaa cacgaaggag actgagtgtg ttaggtatat tccatacttt
      901 ttttcccacg aaaatgttcc tcctgaaaga tgatttgcga gtacagtgta gttgataaag
      961 caaaaaaggg caaccggaaa aagtcccggc tacccttctc actgcataaa aaaacaattt
     1021 atccccccag ataggcctct tttacacggg catccttgag gagctcttct cccttgcctt
     1081 ccaatacaat gttcccgacc tcaagtatat aggcgtagga agcgatctta agggctgcaa
     1141 aggcgttctg ctccacaagg agcactgttt ttcccagagc gtttatctcg ccgattattt
     1201 cgaagacctc tttcacaagg agcggcgcga gtcccagcga gggctcgtcg agcataacga
     1261 gcttcggctg gctcataagc gcgcggccca cggcgagcat ctgctgctct cctcccgaca
     1321 gagtcccgcc cttctgccaa gagcgctctt tcaaccgggg gaagagctcg tagacatgat
     1381 ccctgtctcg ttctattccg tccttgtcat tccggataaa ggcgccgagc tgaagatttt
     1441 cctccacagt aaggtgtggc agaatacgac gcccttcggg actcatggca atcccaagct
     1501 tcaccatttc ttccggagtt ttgccgatga ggtttacctg ccccgaagca tcgccgccgg
     1561 agggcgtgta gagaatcttc cccttgacgt tttttacaag tcccgccaca gcgcggatgg
     1621 tgctgctctt tcccgcgccg ttcgctccga taagagtgac gatggcgcct tcaggaattt
     1681 caaggttgat ccctttcaca gcgtgaatgg cgccgtaata cacatggaga ttctcaattt
     1741 tcaacatatt ttacatactc ctctccaagg taggccacga tcaccttctg gtccgactgg
     1801 atagcgtcgg ggttgccctc ggcgattttt acgccgtagt caagaaccca gatatactcg
     1861 cagacgccca tgacaacttt catatcgtgt tcgataagaa ggatggtcag gttaaagcgc
     1921 ttctgtatat tccggatgaa cgacatgagc tcctgcgatt cctgcggatt cataccggct
     1981 gcgggttcgt cgagcagcag aaatttggga tcggtcgcaa gagcgcgtgc aatctcaagg
     2041 cggcgctgcg ctccgtagga gagggaagct gaaggttcgt ccgcaaaatc ctccaagccc
     2101 acagacttca agagatccat cgatttttcg cgaatggcct cctcttccgc cctgaacgag
     2161 ggaagcccca gcggagccat ccaccatttc gactttctgc ggacgtgaca gccgatcatg
     2221 acgttttcca gaaccgtctc gtttgtgaag aggcggatat tctggaatgt cctcgatatc
     2281 cccgaacggc agaccatatg cggctgaaac cccgttatat cctgtccgtc aaagaagata
     2341 cgtccttcgg taggacggta aaagcctgta atcatattga aaacggtcgt cttgcccgcc
     2401 ccgttcggtc cgataagacc gactattttt ccctgcggca cgtcgatgct gaaattcccc
     2461 accgcagtga ggcctccgaa gcgcatggtg agattctcag tccgaagtat ggatgcggga
     2521 gtcgtcattt catttttcct cccttttctt catgttcggg gcttcgtctt ccgcgagaga
     2581 aaaacgtatc ccatgttatc tcacgcattc ccataatccc ctctctgcgg aagaggatga
     2641 taagaatgag cgcgagggaa aagatcacca ttctcattcc aggaataccc ggaatctcaa
     2701 aggagccgat cactaagggg ttctcgacaa agcgaagcca ttcaagaaga atggtgatga
     2761 cgatacttcc gatgatgctg cccgtaattg accccaaacc tcccgcaacc gcgatcatga
     2821 ggatattgaa cgtgaggacg aacatgaaca tcctcggatc tatcgtggtg atgagacttc
     2881 ccatgagagc gccgcctaca ccggcaaaaa aagcgcctat tgtgaacgcg agcaccctgt
     2941 agaagaaggt attgattccc atgtttttcg ctgcgatttc atcatcgcgg atcgctttca
     3001 ggacatttcc gaagttactg ttcacgaggc ggatgataaa gaagagagtc agaaggagcc
     3061 aggcataatt ccaccagata ttcgcatagg ggggaattcc cttaataccg agggcgccgt
     3121 tggtaataaa gctcaggttg gtgagcacca cacggatgat ctccgcgaag cccagcgtgg
     3181 cgatgccaag gtaatctccg ccaaggcgca gaacaggaat ggcgataagg agcgcgaaga
     3241 aagccgcaat caaaccgccc gaaagcaccg cgacgaaaaa cggcgccttg atgactgaaa
     3301 gaggccatat caagggctca agaatccaca tagcctcttt ctgggccggc gaaagaataa
     3361 gaattgagca tacgtatgcg cctattgcca tgaaccccgc atggccaaga gagaacattc
     3421 cggtaaaacc gtagatgaga ttcagactga gggcaaggat tgcgttgacc gcgatgaggt
     3481 tgagcacctg aatcttgtaa ccgtcgagag tattctgagc ccagtagaga aatgcgccga
     3541 gagcggcgat aaggaggcag ttaagtatga aattgcgcgt cctggtgttc atcagatttt
     3601 gtcctccagc ttctctccca tcagccccgt gggcttcagg agaaggatga cgatgagcag
     3661 cacgaaggca aatgcgtcac gatagcccga caggtgcggg aaaaaggcga cagccatgat
     3721 ttcgatgagc ccgagcagca tacctccgat catagctccc tggatggagc ctatgcctcc
     3781 gaagacggcg gcaataaagg ctttcaaccc cgggacaagt cccatgaaag gatgaatctg
     3841 cggatacctg agagcccaca tgattcccga agccgctgcg agcgcggaac cgatgccgaa
     3901 ggtaaaggcg atgatcttgt tcacggaaac gcccatcagg cgtgtggttt cgatatcctt
     3961 cgatatcgcc ctcatggcca gcccgggttt cgtccggtac acgatccaca agagaccgag
     4021 caccaggagc accgtcacaa tgggaacgac gatagccaga ggcaggatac ggacatttcc
     4081 gattttaaag accttcacaa gccagtcggg ccttgccaca ggacgcggaa taccagtgaa
     4141 gatcacgatg ctcaggtttt gaatcaggaa cgaaacgccg attgcgctga tgagcgccga
     4201 tatacgggga gcgtcgcgaa gaggcttgta cgctacttta tcaacaaaaa taccgcagag
     4261 agaggacccc aaaatagcca cgaccactgc aagtccccat ggaagattca aaagggtgat
     4321 cgcgtagaaa accagatatg cccccagcat gaagatctcg ccgtgggcga aatttatcag
     4381 acgcaaaata ccgtagacca ttgtatagcc gatagcgata aggccataga gagagccaag
     4441 tgtaatcgca ttgaaaaggt gctggatgaa catgctaccg ctcattggag atcccccttg
     4501 cttcaagaaa atggagggtg gaggctggaa cctccgccct ccgtgaaaag gcgcattttt
     4561 gtctccgtcc agtaaaaatc acctttggag acatcggaaa ctcttcccta aagctggggc
     4621 tccacggttc cgatgaactg ctttttgccg tctttaatct ggacgatacc cacagggctt
     4681 tcagcgtcgt gggtcgcatt gatcgtcttg ctcccggtca cgcccgggaa gtccttcgtc
     4741 gaagagagag cggtgcggat tgcatcgggt tctgcgcttc cggcgcgctt gatcgcatca
     4801 acgacgagca tgtaggagtc gtatccgagc gccgcattga cgttgggcgc cttgtccgga
     4861 ttattcttcg tccaattctc ggtgaaggtc ttcgcgatgg ggctcatatc agtcatcgac
     4921 gggtcatagg ggaaggtcgt gtggatgaag ccttcaacag cttctccgcc gatattgact
     4981 atttcggggt tgtccatggc gtcgccgccc atgatctgga actcggcgcc cagctctctg
     5041 gcctgcttca taataatggc gccctcggca aagtcggccg ggatgaagag aaggtcagga
     5101 ttctggctga tgatctcggt aagctgcgca gtaaagtcct gatcgccgga gttgtacttc
     5161 agctccgata cgatctcgcc gccaagcttc ttgtaggact gcttgaagaa attggcaagt
     5221 cccacgcagt agtcgtttgc cacgtcgatg agaagcgcgg cctttttcat gccgagggag
//
LOCUS       DAOTIL010000071         5220 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminobacteriaceae bacterium isolate AMR_MDS_3026
            NODE_18811_length_5220_cov_3.836399, whole genome shotgun sequence.
ACCESSION   DAOTIL010000071 DAOTIL010000000
VERSION     DAOTIL010000071.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868050
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminobacteriaceae bacterium (wastewater metagenome)
  ORGANISM  Aminobacteriaceae bacterium
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae.
REFERENCE   1  (bases 1 to 5220)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5220)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:19:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,224
            CDSs (total)                      :: 3,198
            Genes (coding)                    :: 3,184
            CDSs (with protein)               :: 3,184
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5220
                     /organism="Aminobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18811_length_5220_cov_3.836399"
                     /isolate="AMR_MDS_3026"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:3034609"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..231
                     /locus_tag="P5201_02425"
     CDS             <1..231
                     /locus_tag="P5201_02425"
                     /inference="COORDINATES: protein motif:HMM:NF024138.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HRV97418.1"
                     /translation="ESANRVALGFVDVALGIKSVADALDLDFIPVAEEPYELVYLKEF
                     ESLTGLKAVLNALDDKEWRAGVLRMGGYRWAE"
     gene            219..1277
                     /gene="moaA"
                     /locus_tag="P5201_02430"
     CDS             219..1277
                     /gene="moaA"
                     /locus_tag="P5201_02430"
                     /EC_number="4.1.99.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013048227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP 3',8-cyclase MoaA"
                     /protein_id="HRV97419.1"
                     /translation="MGRIMRDPYGRTLDYVRISITDRCNFRCLYCMPANGIAWIPHED
                     IMTYEDIFFLVSVLSELGVKRLRFTGGEPFVRKGFVSFLQEIRQRFPSIDVAVTTNGA
                     FLERDASALSALRLHSVNVSLDTLHPQKFADITRTGELGTVLRGIRALVAKGGVSVKI
                     NTVVMRDFNLNEISGLVRFAKENDVLLRFIEFMPLDKDVWSNQQYVSASEILSLLPDA
                     LQWSPSPKSSVTTQNTSSAIPNGPAKYFRNAKTGQDIGIISAVSDHFCHTCNRLRITS
                     TGEVRPCLFSNRGVAIIDALRRKDPDAARDGILMAVKNKPHEGGTGIPEERQNPEDAP
                     QKRTDLFQEERHMSRIGG"
     gene            1285..1809
                     /gene="moaC"
                     /locus_tag="P5201_02435"
     CDS             1285..1809
                     /gene="moaC"
                     /locus_tag="P5201_02435"
                     /EC_number="4.6.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012869631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic pyranopterin monophosphate synthase MoaC"
                     /protein_id="HRV97420.1"
                     /translation="MSIIEDEKNELTHLDEQGRPLMVDVGEKPFSARTAEAEGWVLLD
                     QAICDLLGDKGLSKKGDVLRIAETAGIMAVKKTWELIPLCHSIRVDSVRVQCELLADI
                     RKIHIKSIVKANDVTGVEMEALTGVSVAALTVYDMCKAVSKGMVIDGIRLLRKTGGKS
                     GDYIASTPIQVEDV"
     gene            1904..2638
                     /locus_tag="P5201_02440"
     CDS             1904..2638
                     /locus_tag="P5201_02440"
                     /inference="COORDINATES: protein motif:HMM:NF013183.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030151 - molybdenum ion binding [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MogA/MoaB family molybdenum cofactor
                     biosynthesis protein"
                     /protein_id="HRV97421.1"
                     /translation="MNGTEKPFFLLSPGEREKLPEKSVGFVLPKDALKFSYFLSSGED
                     VLFRILEGNPLPRLSVEQGGFISVSHRMQLLRPISVGILTVSDKGSRGEREDLSGPAL
                     ARSVKPLGMFVEEVAIVPDEIGIISDTLTKWSDTGRLNLILTTGGTGLSKRDVTPEAL
                     LSVAEKHVPGIGETMRSVSMKITSKAMLSRGCAVIRGETLIIALPGSEKAAEECFAAI
                     APALRHGIEILCGWDSECGSRPHHHH"
     gene            2706..3587
                     /gene="rapZ"
                     /locus_tag="P5201_02445"
     CDS             2706..3587
                     /gene="rapZ"
                     /locus_tag="P5201_02445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008711599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNase adapter RapZ"
                     /protein_id="HRV97422.1"
                     /translation="MDAVGVNKCIIITGMSGAGKSTALKILEDQGMFAIDNIPPVLLS
                     QLLFLLEKHRAAVRWGVAAVVDIRGDSLLDDFPAALALLRKQTRHVHILFFDAKDEIL
                     LRRFETTRRRHPLEPDGSFLDGIAKERERLQPILDYADTIIDTSALSLSAIRKELISR
                     FFEDAAAVPLVISSFGFKHGAPADCDYVFDVRFLDNPFYNPVLKPLSGKDEPVQEEIW
                     RSVEARGFWEKLISFLEYIVPLYLRSGKAHLHIGVGCTGGRHRSVAVAERLYAHFKEY
                     SGGCILRHRDIEKDQGW"
     gene            3590..4711
                     /gene="yvcK"
                     /locus_tag="P5201_02450"
     CDS             3590..4711
                     /gene="yvcK"
                     /locus_tag="P5201_02450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013048231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uridine diphosphate-N-acetylglucosamine-binding
                     protein YvcK"
                     /protein_id="HRV97423.1"
                     /translation="MDLLISYGLGLFTAIILVFLFRLRYSGRTGQLLQRAPQERSTAI
                     SKAIEYRLSMGPCVAALGGGTGLSTLLRGLKSFTRNIVAVVTVTDEGGSSGKLREEWG
                     VLPPGDVRNCIVALAENDNALKRLLDFRFDRGALAGHSLGNLMLLAISEQFGDFRLAV
                     EEMNHLLAIRGRVLPVTTESVSIVAETRDGSRIRGELSVSEHGSQIHKIWLEPRDAKP
                     LKEVLLSLDEADIIVLGPGSLFTSIIPNLLIRKVAERLRQSEAPKVYIANLMTQPQET
                     QGFNILDHVRWISAAMGVSPDYVLVNEGVIPHLLEEKYRVEGAEPLYITEEEEWELEK
                     MGCKILRGDFVRIMDGNVVRHNGQALSEILIRLCRELDE"
BASE COUNT         1272 a         1291 c         1396 g         1261 t
ORIGIN      
        1 gaatcagcca acagagtagc gttaggcttt gtcgacgttg ctcttggtat aaaatctgtc
       61 gccgacgcgc ttgacttgga tttcattcca gttgcggaag aaccctatga actcgtctac
      121 ttgaaagaat tcgaaagcct tacaggactg aaagccgtgc tcaacgcatt ggacgataaa
      181 gagtggcgcg ccggcgtact gcgtatggga ggataccgat gggcagaata atgcgtgatc
      241 cttatggaag aacgcttgac tacgttcgta tttctatcac cgaccgctgt aattttcgct
      301 gtctttactg catgccggca aacggtattg cctggattcc tcatgaagat attatgacgt
      361 acgaagacat attctttctc gtctccgttc tttctgaact tggagtaaag aggcttcgtt
      421 tcaccggcgg agagcctttt gtcaggaagg gattcgtttc gtttctgcaa gaaatacggc
      481 aaagattccc ttccatcgac gtcgcggtaa cgacaaacgg agcgtttctg gaacgggatg
      541 catcagcact ttctgctctc cgccttcatt cggtgaatgt gagtcttgat acgttgcacc
      601 cgcagaagtt cgctgacatt acgaggacgg gggaactcgg aacagtgctc cgcggaattc
      661 gcgccctcgt tgcaaaggga ggagtttctg taaagataaa cacagtggta atgcgtgatt
      721 tcaacctcaa tgagatttcc gggctcgttc gcttcgccaa agaaaacgat gtactgctca
      781 ggtttatcga attcatgccg cttgacaagg acgtctggtc aaaccagcag tacgtctccg
      841 cctcggagat tctctcgctt ctcccggacg ctcttcagtg gtccccctca ccgaagagct
      901 ctgtgacaac gcagaacaca agctctgcaa ttcctaacgg cccagcaaag tatttcagga
      961 atgcgaaaac agggcaggat atcggtatca tctcggctgt ctccgatcat ttctgccata
     1021 cgtgcaatcg ccttcggata acatcaactg gtgaagtgcg cccttgcctc ttcagcaaca
     1081 gaggcgtcgc catcatcgac gctttgcgaa ggaaggatcc cgacgccgcc cgcgatggca
     1141 ttctcatggc ggtaaaaaat aaaccccatg aagggggtac gggtattccc gaagagcggc
     1201 aaaacccgga agacgctcct caaaagcgta cagatctctt ccaagaagaa cgtcacatgt
     1261 cccggatcgg aggatgaact ctctgtgagc attattgaag atgaaaaaaa tgaattgacg
     1321 catctggatg aacaggggcg gcctctaatg gtcgatgtcg gcgagaagcc cttttccgcc
     1381 cgcacagcgg aggcggaagg atgggttttg ttggatcagg caatctgtga tctcctgggc
     1441 gacaaaggcc tctcaaaaaa aggagatgtc cttcgtattg cggaaactgc cggaataatg
     1501 gccgtgaaaa aaacgtggga gctgatccct ttatgtcact cgattcgagt cgacagtgta
     1561 cgagtgcagt gcgagctgct ggcggacatc cgaaaaatcc atatcaagag catagtgaag
     1621 gcaaatgacg ttacgggagt tgaaatggag gcgcttaccg gcgtttcagt agcggctctc
     1681 acggtgtacg atatgtgcaa agcggtctcg aaagggatgg ttattgacgg catccgtctt
     1741 ctccgtaaaa ccggaggaaa aagcggcgac tacatcgctt cgactccaat tcaagtggag
     1801 gacgtttgac atgaaagtcc tcgctattat tgaaaggtct acccgtacct ttctcccgca
     1861 cacgctcagt tacgtaagca ttaacaaagc cggagaaagc gccttgaacg ggacagaaaa
     1921 gccgttcttt ctgctctctc cgggagagag agaaaaactt ccggaaaaga gcgtcggctt
     1981 tgttctcccc aaagacgcgc taaaattctc ctatttcctc tcttccggag aggatgttct
     2041 ctttcgcata ttggaaggga atccgttgcc gcgattgagc gttgaacagg gaggtttcat
     2101 ctccgtttca catagaatgc agctgctccg tccgatttcg gtcggcatac tcaccgtgag
     2161 tgacaaaggg agcaggggag agagggagga cctctccggt ccggctctag cccgtagcgt
     2221 taagccgctc gggatgtttg tcgaagaagt tgcgattgtc cctgatgaga tcggaatcat
     2281 ctcggatacg ctcacgaagt ggagcgatac cggccgtctc aatctcatac tgactacagg
     2341 cggaaccggt ctgtccaaga gagatgttac tcccgaggcg cttctttccg ttgccgaaaa
     2401 acatgttccg ggcatcgggg aaacaatgcg cagcgtttcg atgaagataa cctcaaaagc
     2461 catgctgagc agaggctgcg ccgttatccg tggagaaacg cttattattg cccttccagg
     2521 gagcgaaaag gctgcggaag aatgcttcgc tgcaatagct cccgcactgc gccacggtat
     2581 agagatactg tgcggatggg acagtgaatg cggctcccgc ccgcatcatc atcattgagc
     2641 agcccgataa cagagaaaat atacattggg gcgcaaatct gtatgaaggg agacgaagcg
     2701 tggatatgga tgccgtcggt gtgaacaagt gcattattat cacgggtatg tccggcgcag
     2761 ggaaatccac cgcgctgaaa atcctcgaag atcaaggtat gtttgcaatt gacaacatac
     2821 ctcctgttct gctttctcaa ctgctctttc ttctggaaaa acatcgggca gccgttcgct
     2881 ggggagtcgc agcggtcgtg gatattcgcg gagattcgct gctggatgat ttcccggcgg
     2941 ccctcgcctt gctgagaaaa cagacgcgtc atgtgcatat tctctttttc gatgccaagg
     3001 atgaaatttt gctgcggcgt tttgaaacca cacggcgccg ccatcctctg gaacccgacg
     3061 ggtcttttct tgacggtatc gcaaaggaac gcgaacgcct acaaccgatt ctcgactacg
     3121 ccgatacaat catcgatacc tcggcgctct ctctttctgc aattcggaaa gagctgatct
     3181 ccaggttttt tgaggatgcc gcggccgttc cgcttgtgat ctcatccttc ggtttcaaac
     3241 acggagctcc tgctgattgc gactacgttt tcgacgttcg tttcctggat aatcccttct
     3301 acaatccggt tctcaaaccg ctttcgggga aagacgaacc tgttcaagag gagatctggc
     3361 gttcagttga ggctcgcggt ttttgggaga agctcatttc ttttctcgaa tatatcgtcc
     3421 ctctgtatct ccgttcaggg aaagcgcatt tacatatagg cgtcggctgc acaggaggac
     3481 gacatcgctc agtggccgtt gcggaacgtc tctacgctca tttcaaagag tattcaggcg
     3541 gatgcattct tcgtcacagg gatattgaaa aagatcaggg gtggtgatta tggacctgtt
     3601 gataagctat ggactcggtc tttttaccgc cataatcctc gtctttcttt ttcgtttgag
     3661 atactccggg cgtaccggac agcttcttca gcgagcgccg caagagcgca gcacagccat
     3721 ctcgaaagct atcgagtatc gcctttcgat gggaccttgt gttgcggcgc tcggcggagg
     3781 taccgggctt tcaacccttc tgagagggct aaaaagcttt acacgcaata tcgtggcggt
     3841 tgttacagta actgacgaag gaggcagttc gggaaaactc cgcgaagagt ggggagtcct
     3901 ccctccgggc gacgtccgta actgcatagt tgctcttgca gaaaacgaca atgcgctgaa
     3961 gcgccttctc gactttcgat ttgatcgcgg cgccttagca gggcacagcc tcgggaacct
     4021 gatgctgctc gctatctccg agcagttcgg cgacttccgc ctcgcggtag aagaaatgaa
     4081 tcatctgctc gctattcgcg gcagagtcct cccggtaact acggaatccg taagtatcgt
     4141 ggctgaaaca cgggacggtt cgcgcatccg aggggagctc tctgtgtccg aacatggatc
     4201 tcagattcac aaaatctggc ttgaaccaag agacgccaag ccgctcaagg aagtgctgct
     4261 ttcgcttgat gaggccgaca tcatcgttct gggtccggga agcctcttta ccagtatcat
     4321 tccgaacctc ctcattcgaa aggttgcaga aaggctgcgc caaagtgaag ccccaaaagt
     4381 ctatattgca aatctcatga cgcaacctca ggaaacccaa ggattcaaca tacttgatca
     4441 cgtccgctgg atcagcgcgg ctatgggggt atctccggat tacgtcctcg tgaacgaggg
     4501 tgtgattcct catcttctcg aagaaaaata ccgtgtagag ggtgcggaac ctctttatat
     4561 tactgaagaa gaggagtggg agctggaaaa aatggggtgc aagatactac ggggagactt
     4621 cgttcgaatc atggacggta atgtagtgcg acacaacgga caagcgctct ccgagattct
     4681 gatacgactg tgccgggaac tggatgagtg atgaaaccga tagattcccg ctcaacgctg
     4741 acgtcaagca tgtgggacga atggacatcg tcaatagcct cccccggtcg tgctgtcgag
     4801 gatgaaatca gagggatagt gtccggtctt tcagaggtgg catctcatga aagggaaact
     4861 gttttttcga gtggacgcct ttggattttt cgacgcctca gaatgctctg gtctgcatgg
     4921 gaaaacgcac ggaattatga attcgcttcg ctcctcaaca tcccgcgttc aatgaaagga
     4981 cgcgtttttt tccgtcttcc ttcagaaatt tatgcgacgt tccgcgaacg agaatctgaa
     5041 tctcgaaata cgccctggct gagggggttg tggggaagtt gcggctcttt gtatttgccg
     5101 aagacaggct attatctctg ttttcgaatc tcctcccgga taacggagga gaaaactgca
     5161 catcttttgc agaggaacgg atttacagct ggaagaagaa aaattcaggg gaaatatgaa
//
LOCUS       DAOTIL010000072         5208 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminobacteriaceae bacterium isolate AMR_MDS_3026
            NODE_18864_length_5208_cov_3.500485, whole genome shotgun sequence.
ACCESSION   DAOTIL010000072 DAOTIL010000000
VERSION     DAOTIL010000072.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868050
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminobacteriaceae bacterium (wastewater metagenome)
  ORGANISM  Aminobacteriaceae bacterium
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae.
REFERENCE   1  (bases 1 to 5208)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5208)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:19:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,224
            CDSs (total)                      :: 3,198
            Genes (coding)                    :: 3,184
            CDSs (with protein)               :: 3,184
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5208
                     /organism="Aminobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18864_length_5208_cov_3.500485"
                     /isolate="AMR_MDS_3026"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:3034609"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..687)
                     /locus_tag="P5201_02455"
     CDS             complement(<1..687)
                     /locus_tag="P5201_02455"
                     /inference="COORDINATES: protein motif:HMM:NF040244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase modulator"
                     /protein_id="HRV97424.1"
                     /translation="MKKFPERTAAESAASFLIERAVKRGASAADVLYTFNSGHSLSLL
                     DGEPEKISSGFSLGIGLRSLDSAGRQGVAHVNSLDRNLLEEVVEWSFSNCMSSEPDAF
                     VALSDPIPACTEDLALTDEAVLNLSSSDRMAFCREMWETARSADPRVVSVRSASWGDG
                     AGEVLYASSTGAMMWYEGASAGCGVSVILSSGDEMEMGGYGDESRFLSSLQPRATALE
                     AVRRTAMVLGG"
     gene            complement(684..1715)
                     /gene="ltaE"
                     /locus_tag="P5201_02460"
     CDS             complement(684..1715)
                     /gene="ltaE"
                     /locus_tag="P5201_02460"
                     /EC_number="4.1.2.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005659250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="low-specificity L-threonine aldolase"
                     /protein_id="HRV97425.1"
                     /translation="MFPVDLKSDTVTKPSEEMRKAMYAAEVGDDVYGEDPTVNRLEET
                     AAALTGMEAALFLPSGTMGNLVAVLTHCGRGDGAILGADCHILNYEGGGLAALGSVVP
                     LVADDSSGVISPEEVRAHCRPSNVHFAPARLLCLENTHNRRGGLALSPEAVRSSAQEA
                     HRLGLSVHIDGARIFNASTAWSCSVKKYAESVDSLQFCISKGLGAPVGSLLCGRRSFV
                     DAARHWRKRLGGGLRQSGILAAAGLYALEHNIERLREDHENAELLAHLLSEGSVVKVE
                     HNEKPTNMVYCAVPDGACADLHLRCADRGVLFNGVAENRFRLVTHLDVSREQVTRAAA
                     IILEEAAAS"
     gene            complement(1895..2767)
                     /locus_tag="P5201_02465"
     CDS             complement(1895..2767)
                     /locus_tag="P5201_02465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013047700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PHP domain-containing protein"
                     /protein_id="HRV97426.1"
                     /translation="MIRIDLHLHSTFSDGSMTPEALVAHGRSKGVTVMSLTDHDTVDG
                     LPSFMAACARWNMAGIPGIELSADHARTVHILGYRLDLRRAGSASSASAQGNTPSMGM
                     KKMLEEVRRGRDERNAAMCENLRRMGLDITLDEVALEAGGDVVARPHMARVLVRKGYA
                     QDIVSAFARYLNTSSPAYVERYRLSPEDCIRMIADAGGLAVLAHPAQTTGDLSELREI
                     VRMLKDAGLWGIECVSSRASAEQIFRYLSLAAEFSLFPTAGNDFHGANRPSAMMGVAV
                     AEDFLPWARLGVSL"
     gene            complement(2779..3303)
                     /locus_tag="P5201_02470"
     CDS             complement(2779..3303)
                     /locus_tag="P5201_02470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005659255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheW"
                     /protein_id="HRV97427.1"
                     /translation="MNPTLATKETQDLKAREVESRGTERITLVFALYEEDFGLDVTYV
                     REIVRVPPFITRVPNSPDYIRGVINLRGSIVPVFDMELKIGMMQKELTEEARIVVVSW
                     NEILFGIIVDSVREVCTIYDSQIESAAKLNTTMDKKYLLGVAKHEDGRLIVLLDLAAL
                     FDIDQILDEEAFNG"
     gene            complement(3341..>3846)
                     /locus_tag="P5201_02475"
     CDS             complement(3341..>3846)
                     /locus_tag="P5201_02475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013047699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HRV97428.1"
                     /translation="DTHGHEDAWMKASLLWGNSLDAVLHAGDVLSSDVDTGLAGMIRT
                     APFPVIISRGNCDYPEDEKLLHRPILSPYAFVWWNGKSILMAHGNAFEQFRSLALRSR
                     VDLAVYGHTHVASVVREEGTLFVNPGSASLPRGRDPASAALIDEDGVVIFTLDGAELH
                     REQWIYS"
     assembly_gap    3847..3856
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            4027..4413
                     /locus_tag="P5201_02480"
     CDS             4027..4413
                     /locus_tag="P5201_02480"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRV97429.1"
                     /translation="MTEERNEHKLIQEVKRLLDSEGLVTARLVESVTRTAQMASTTVP
                     EIQDLFSQWLSLIGGEVARMAASSPEMDIASTANRIGIEESSLLSLLLMLQREGALSI
                     ESVRIGKGKGKNSEICSCLMGTEEDA"
     gene            4410..5174
                     /locus_tag="P5201_02485"
     CDS             4410..5174
                     /locus_tag="P5201_02485"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRV97430.1"
                     /translation="MTTDLELLSKKARRQAAMAEAGLLPHPGQEELLQRIEYVFITPA
                     PEEAALLNACRAASANMLASLCIPSPWIGKASQFLLESKTALSSVVAFPFGFSPVEAK
                     KIEAFAAAAAGAQEIQVSLSPHTLKIHDRNGLRREMEELRSALVKSELTFIIETSLLS
                     DEEIITIVRAAELARAERVKGGSGFWGATSLHGAAILRCAAPDWMGVTAVLEAGNAKN
                     ALPLLAAGADRIATSAPQDLPGFEDISRQEETPQSL"
BASE COUNT         1163 a         1571 c         1444 g         1020 t
ORIGIN      
        1 gccgccgagc accatggcgg tacgcctgac agcctccaga gctgtcgcac ggggctgcag
       61 agacgaaaga aatcggcttt cgtccccgta tccgcccatc tccatctcgt cgccggatga
      121 aaggatgaca gagacgccgc atcctgcgct tgctccttcg taccacatca tcgcccctgt
      181 cgaagaggcg tagagcacct ctccggctcc gtcgccccag gaagcgctgc ggacggagac
      241 cacccgggga tcggcgctcc tggcggtctc ccacatctcg cggcagaacg ccattcgatc
      301 cgaggaagag aggttcagta ccgcttcatc agtgagagcg agatcctccg tgcatgcagg
      361 gatcgggtcg gacaatgcga cgaacgcatc cggctccgag gacatgcagt tggagaaact
      421 ccattccacc acttcttcaa gaagattccg gtcgagagag ttgacatgcg cgacgccctg
      481 ccgccctgcc gaatcgagcg aacgaagacc tatcccgagg gaaaaaccgg aggatatttt
      541 ttcgggctct ccgtcgagca gagaaaggga gtggccggaa ttgaaggtat acagcacgtc
      601 cgccgccgac gcccccctct ttactgctct ttcgatcagg aaggaggcgg cagattcggc
      661 cgccgtcctc tccggaaact ttttcacgaa gcagcagcct cctcgaggat gatcgccgcg
      721 gcccgggtta cctgttctct cgacacgtcg agatgcgtca caaggcggaa acggttctcc
      781 gcaacgccgt tgaacaggac gcctctgtcg gcgcagcgga gatgcagatc ggcgcacgcg
      841 ccatcgggaa cggcgcagta caccatgttc gtcggtttct cattgtgctc aaccttgacg
      901 acgcttccct cggagagaag atgcgcgagc agttcggcat tctcgtgatc ctcccggaga
      961 cgttcgatat tatgctccag cgcgtacaga ccggcggcag caagaattcc ggactgccgc
     1021 agtccgccgc caagacgttt cctccaatgg cgggcggcgt cgacaaagct gcgcctcccg
     1081 cacagaaggc ttccgaccgg tgcgccaagc ccctttgaga tgcagaactg aagcgaatcc
     1141 acgctttccg cgtacttctt cacagaacag ctccatgccg tggatgcgtt gaagatcctc
     1201 gctccgtcga tatgcacgga gagaccgaga cgatgggctt cctgcgccga agaccgaacg
     1261 gcctcgggag agagcgccag tcctcctctg cggttatgcg tgttctcaag gcagagcagc
     1321 ctggcggggg cgaagtgaac attcgacgga cgacagtgtg cgcgcacttc ctccggagag
     1381 atcacccccg agctgtcgtc ggcaaccagc ggaacgacgg agccgagagc ggccagaccg
     1441 ccgccttcat aattcagtat gtggcaatcc gcgccgagaa tcgcgccgtc ccctctgccg
     1501 cagtgcgtga gcaccgccac caggttcccc atggttcctg agggcagaaa cagggcggcc
     1561 tccatgccgg tgagcgcggc ggccgtctcc tccaggcgat tgacggtggg atcttcgcca
     1621 tagacgtcat cgccgacttc tgcagcatac atagccttcc gcatctcctc cgaaggcttc
     1681 gttacagtat cgcttttaag atcaactgga aacatgtgtc caattcctcc tttaaaccag
     1741 agcgttcacg aatatgccgt gtggcaatta cattctcaaa tcaacattat acaaaagctc
     1801 cggctgatat aataccatca aaagagcgaa ttccggaggg tagttcccgc cgaaattcgc
     1861 ccgcagcttc atcgaagccc ctgcaagcag gacgttacag agaaacgccc agcctcgccc
     1921 aagggaggaa atcctccgca accgccacgc ccatcatggc ggacggacgg ttggcgccgt
     1981 ggaaatcgtt gcccgccgtc gggaagagag aaaattccgc tgcaagtgaa aggtagcgga
     2041 agatctgctc cgcggaagcc cgcgaggaga cgcattcaat tccccacaat ccggcgtcct
     2101 tgagcatccg gacgatctcc cggagttccg aaagatcgcc ggtagtctgc gcggggtgcg
     2161 cgagaacggc gagccctccc gcatcggcga tcatccggat gcagtcctcc ggcgaaagac
     2221 gatagcgttc cacatacgcc ggagaggatg tgttcaggta acgtgcgaag gcagaaacaa
     2281 tgtcctgagc gtatcctttt cgcacaagca ctcttgccat gtgggggcgc gcgacgacat
     2341 cgccgcccgc ctccagggca acctcatcca gagtaatgtc gagccccatc ctccgcagat
     2401 tctcgcacat cgcggcattc cgctcgtccc ttccccgacg cacttcctca agcatttttt
     2461 tcatgcccat ggaaggcgta ttcccctgcg cggaagcgga agatgcgctc cctgcgcggc
     2521 gaagatccaa tcgatacccc aggatgtgga cggtacgcgc atgatcggcc gagagttcta
     2581 ttcccggaat accggccatg ttccatcgcg cgcacgccgc catgaacgaa gggagcccgt
     2641 cgaccgtatc gtgatcggtc agactcatga cggtcacgcc tttacttctg ccgtgggcca
     2701 cgagggcttc cggcgtcatc gagccgtccg agaaggtgct gtggagatgc aggtcgatac
     2761 ggatcatacg gccccgactc acccgttgaa tgcttcttcg tcgagaatct ggtcgatgtc
     2821 gaacagggca gcaagatcga gcagcacgat cagacgcccg tcttcatgct tcgccacacc
     2881 gaggagatac ttcttgtcca tggtagtgtt gagcttggcg gcggactcga tctgactatc
     2941 gtagatagtg catacttccc gcacggagtc cacaatgatg ccgaagagga tttcgttcca
     3001 ggagaccacg acgatccttg cctcctcagt cagttccttc tgcatcatgc cgatcttcag
     3061 ctccatgtcg aagaccggaa cgatggatcc ccgcaagttg ataacgcccc ggatatagtc
     3121 gggagagttg ggaactctcg tgataaacgg gggaacccgg actatctccc tgacgtacgt
     3181 cacatcaaga ccaaagtctt cctcatacag agcgaacaca agcgtgatac gctccgttcc
     3241 tctgctttcc acctcacggg ctttcaaatc ctgtgtttct ttagtcgcaa gcgtcggatt
     3301 cattttcttt cccctcccgg tagaatatct ctcgaatata tcatgagtat atccactgtt
     3361 ctctgtgcag ctccgcaccg tcaagcgtga agatgacgac gccgtcttcg tcgatgagtg
     3421 cggcgctggc gggatccctc cccctcggaa gagacgcgga accggggttg acaaaaaggg
     3481 tcccttcctc gcgcaccacg gaggcaacgt gcgtatgtcc gtacaccgca aggtcgaccc
     3541 tgcttcgcaa cgcaagactc cggaactgct cgaaagcgtt tccatgcgcc ataaggatac
     3601 ttttcccgtt ccaccataca aaggcgtacg gcgaaagaat gggccggtgc aacaactttt
     3661 catcctcggg ataatcgcag tttccccgcg aaattataac aggaaacggc gcggtgcgaa
     3721 tcatcccggc caatccggta tcgacatccg aagagagcac atcgcctgca tgcaggacag
     3781 cgtcaagaga attaccccac agcaaggatg ccttcatcca ggcatcttca tgcccatgcg
     3841 tgtccgnnnn nnnnnncgac gtttcattat ccggttcccc tccttacaac gggatgttcc
     3901 ccaatacggc aaagtgtact aaaatggaga ggaacaaaag ccccgatact catttattgt
     3961 agcatgctcc gggctttttc atcgcttgcg gagagagtat gaaaaaatac aaaaggagcg
     4021 tatcccatga ctgaagaacg taatgaacac aagcttattc aagaggtgaa gaggcttctg
     4081 gactccgaag ggctggtaac cgcccgcctg gtggagtctg tcacccgcac cgcgcagatg
     4141 gcctcgacaa ccgtgcctga gatacaggat ctcttttccc agtggctctc gctcatcgga
     4201 ggggaagtcg cacgcatggc ggcttcctcg ccggaaatgg acatcgcgtc cacggcgaac
     4261 aggatcggca tcgaggagtc ttcccttctc tcccttttat tgatgctcca gagagagggc
     4321 gcactctcga tagaatcggt tcgtatcggc aagggaaaag gcaagaacag cgagatttgt
     4381 tcgtgcctta tgggaacaga agaggacgca tgacgaccga cctggaactc ctttcgaaaa
     4441 aagcacgaag acaggccgcc atggctgaag ccggcctgct tccccacccc gggcaggaag
     4501 agcttttgca gcggatagaa tacgtgttta tcacccctgc acctgaggag gccgcgctgc
     4561 tcaatgcgtg cagggcggcc tctgcgaaca tgctcgcgtc cctctgtatt ccctccccct
     4621 ggatcggaaa ggccagtcag tttcttcttg agagcaagac tgcgctttcc agcgttgtag
     4681 ccttcccctt cgggttttcc ccggtcgaag cgaagaaaat cgaggcgttc gcggcggcgg
     4741 cggctggagc acaggagatt caagtatctc tttcaccgca cacgctgaag atacacgacc
     4801 ggaacggact cagaagagag atggaggagc tccgctccgc ccttgtcaag agcgaactga
     4861 cgttcatcat cgagacttcg ctgctctcgg atgaggagat cattactatt gtcagggcgg
     4921 cggagctcgc gagggcggaa agggtaaaag gaggatccgg tttttgggga gcaacgtcgc
     4981 tccatggagc cgctatcctg agatgcgcgg ccccggactg gatgggcgtg actgccgtgc
     5041 tcgaagccgg aaatgcaaaa aacgcgctgc cgcttcttgc cgcaggagcg gatcgtatcg
     5101 ccacatccgc accgcaagac ttgccgggat ttgaggatat ctcccggcaa gaagaaacgc
     5161 cgcaaagtct ttaaaggcac atatacacag gaagccgtcc tgaggttt
//
LOCUS       DAOTIL010000073         5198 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminobacteriaceae bacterium isolate AMR_MDS_3026
            NODE_18916_length_5198_cov_4.002722, whole genome shotgun sequence.
ACCESSION   DAOTIL010000073 DAOTIL010000000
VERSION     DAOTIL010000073.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868050
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminobacteriaceae bacterium (wastewater metagenome)
  ORGANISM  Aminobacteriaceae bacterium
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae.
REFERENCE   1  (bases 1 to 5198)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5198)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:19:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,224
            CDSs (total)                      :: 3,198
            Genes (coding)                    :: 3,184
            CDSs (with protein)               :: 3,184
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5198
                     /organism="Aminobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18916_length_5198_cov_4.002722"
                     /isolate="AMR_MDS_3026"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:3034609"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(41..559)
                     /locus_tag="P5201_02490"
     CDS             complement(41..559)
                     /locus_tag="P5201_02490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014162546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 glutamine amidotransferase
                     domain-containing protein"
                     /protein_id="HRV97431.1"
                     /translation="MSKKIAVLTEETIHDLEFWYPYYRLAEEGYDLVIVGPEAGKSYT
                     GKMGGVIRSEKSPSEISAKDFAGVVVPGGWGPDKLRTHKAIVDFVRDVHANGGVVAAI
                     CHGGSVLVSARILEGVRATSYKSIKDDMILAGAEWVDEAVVVSKNIVTSRTPSDLAHF
                     GKALVETLKKIK"
     gene            651..1178
                     /locus_tag="P5201_02495"
     CDS             651..1178
                     /locus_tag="P5201_02495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013047662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HRV97432.1"
                     /translation="MTLRDNSVIRAILSRRSIRKFRSDAVSPEMRDLLLECAFAAPSA
                     HNRRPCHFIVLEDRALLDAVAEVHDSGKMLFQAPLAIAVCAETASYPEGDLAWVEDCS
                     AALENILVAAKGLGLDGVWLKVMGRHPRNELVRPVLGVPESVQIVGIAALGFGAEEKS
                     PCEGIKEDRVHKNRW"
     gene            1315..3792
                     /gene="gyrA"
                     /locus_tag="P5201_02500"
     CDS             1315..3792
                     /gene="gyrA"
                     /locus_tag="P5201_02500"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006299964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006259 - DNA metabolic process [Evidence
                     IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase subunit A"
                     /protein_id="HRV97433.1"
                     /translation="MDEQKSEMPIGKVLPLPLVEEIKHSYLDYAMSVIVGRALPDVRD
                     GLKPVQRRILYAMMELGLRSGSSYKKSARVVGETMGKYHPHGDAAIYETMVRMAQDFS
                     MRYPLVDGQGNFGSIDGDPAAAMRYTEAKLFETGELMLADIDEDTVEWGPNFDESLKE
                     PLTLPSMIPNLLVNGSSGIAVGMATNIPPHNLCEVVDALEYLIEADGNIEFGEIYARL
                     PGPDFPTGGVILGRNGIIDAYRTGRGKIILRGKTHVEEGKKGKTSIVITEIPFMVNKT
                     LLIETIASCVQEKHIDGISDIRDESDRDGLRVVLELQRDADAELVLRQLYRRTQLQST
                     FGVINLALVENSPKELPITDMLSLFLEHRRDVVRRRTQFRLDKALARAHIVEGLVKAL
                     DIIDQVIATIRAAQTTQEAREGLVDTLGFSELQAQAILEMRLQRLTGLEREKLDAELA
                     ALLADIERFRSILGNAKILDGVIKDELSLLRKKFGSPRRTEIIDSYEEVSIEDLIPEE
                     DIVVILSKDGYLRRKSLDEYVPQARGGKGRKGAALQDEDEIAVFAVTNTHKDIYLFTS
                     SGRVLAIRGHMIPESRSGKGKLVGKLVPLEDGETVVSMYGRSLEGKSYLFFATKNGIA
                     KRLQLSEILRINRGGKRVLTLDEGDEIAQVRLTSGDDELLFMTAAGQALRVPEQEFRA
                     MGRAARGVKGIRLAKDDSVISCEVVNKERHALVISEKGVAKRTSFEEFTLHHRGGSGV
                     KAMNLGRKTGMLVGSWSVAEEDEIMAITARGRIIRVAVSEIPVLSRTAMGSITVRLDG
                     GDSVADVSVVCGEVCVAAEIPYEEETE"
     gene            3789..4409
                     /locus_tag="P5201_02505"
     CDS             3789..4409
                     /locus_tag="P5201_02505"
                     /EC_number="4.1.1.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006299965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylserine decarboxylase"
                     /protein_id="HRV97434.1"
                     /translation="MKLAREGYPLIAFTVFMIIGSVFFAPPVALFLLPVLGLVVWFFR
                     DPERFPDGEGFLSPADGRIVEIEQADLPFTGKAWKIGIFMNPLSVHVNRFPCAGTVEH
                     LEYVPGKKWMAFAPKASEVNERMLVGISTEYGPVLLVQIAGFLARRIVCRLRKGDRLE
                     RGQRYGMIKMGSKVDVYLPAKTRLAVAKGQQVFAGRTVLGGVSCGE"
     gene            4459..5139
                     /gene="pssA"
                     /locus_tag="P5201_02510"
     CDS             4459..5139
                     /gene="pssA"
                     /locus_tag="P5201_02510"
                     /EC_number="2.7.8.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006299966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003882 - CDP-diacylglycerol-serine
                     O-phosphatidyltransferase activity [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol--serine
                     O-phosphatidyltransferase"
                     /protein_id="HRV97435.1"
                     /translation="MVTSGNLLCGTMSLILVLHGRVVPAAWLVFMAVFFDFMDGKVAR
                     RLGGGTRFGLEFDSLADVVSFGVAPAVLFYSVYAERLGVMGALAVSFFALCGALRLAR
                     FNVTHVPGPFQGLPIPAGGLFLASFVLAGAHLSPWLAALLALGTGALMVSSVPYSSMK
                     KLRRGYARKKRLLLVGGAAISGAILLESAAPLAGISLYVVSGILRFDWEKWLSVPGAE
                     EERIEKSL"
BASE COUNT         1061 a         1415 c         1614 g         1108 t
ORIGIN      
        1 gccccgccgg aggggcgagg ccttgcagga caggaagaaa ctacttgatc tttttgagcg
       61 tttcgacgag cgccttgccg aagtgcgcca gatcggaagg cgtgcggctt gtgacgatat
      121 tcttcgagac gactaccgcc tcgtcaaccc attccgctcc ggcgagaatc atatcgtctt
      181 tgatgctctt gtaggaagtg gcgcgaacgc cttccagaat acgcgccgat acaaggacgc
      241 tgccgccgtg gcatattgcc gcgacgacgc cgccgttggc atggacgtcc ctgacgaaat
      301 caacgattgc cttgtgtgtt ctgagcttat cggggcccca ccctccgggg acgacgacgc
      361 ctgcgaagtc cttcgcggag atctccgaag gtgatttctc cgagcggatc acgccgccca
      421 tcttcccggt atagctcttt cccgcttccg ggccgacgat gacgagatcg tacccctctt
      481 ccgcaagacg gtagtagggg taccagaatt cgaggtcgtg aatggtctcc tctgtaagta
      541 cggctatttt cttggacata ctctgtccct ccttcttttg gtgatacgat tgttgaaact
      601 cttcacgttt atagtctatc ataaaacatc ctttacagga ggcgatgaca atgacattga
      661 gggataattc tgtgattagg gcgattctct cccggaggag tatcaggaag tttcgctccg
      721 atgctgtatc gccggagatg cgcgacctgc ttctggagtg cgcatttgcc gcgccgtcgg
      781 cgcacaaccg gcgtccgtgc cactttattg ttctcgagga tcgggctttg ctcgatgcgg
      841 tggcggaagt ccacgactcg gggaaaatgc tttttcaggc gcccctggcg atcgccgtct
      901 gcgccgaaac cgcatcctat cctgaaggag atcttgcctg ggtggaggac tgctctgcgg
      961 ctcttgagaa catccttgtc gccgcaaagg ggcttggcct cgacggcgtc tggctgaaag
     1021 tgatggggcg tcacccgcgg aacgaactgg tgcgtcctgt tctcggcgta ccggaatcgg
     1081 tgcagattgt gggtatcgcc gcgctcggat tcggtgcgga ggaaaaaagt ccctgcgaag
     1141 gaatcaaaga agaccgcgtg cacaaaaacc gttggtaaat aaacacccgg gaagagcatt
     1201 catgcgtgtt gggacagctc actactcctc ctgacgtgat acaatataca ggttatgctg
     1261 tacacgtcgc cggctttttg cacggcgaat cagctttgga ggttgaagga tcgtatggac
     1321 gaacaaaaaa gtgaaatgcc cattggcaag gtgctccctc ttcctctggt agaggagatc
     1381 aaacacagct atctcgatta cgcgatgagc gtcatcgtgg ggcgcgcgct ccccgatgtg
     1441 cgggacggtc tgaaaccggt gcagcggcgg attctgtacg caatgatgga gctggggctc
     1501 cggtcaggat cctcgtataa aaagtcggcg cgcgtcgtgg gagagacgat gggtaagtac
     1561 cacccgcacg gcgacgcggc gatctacgaa accatggtcc gaatggcgca ggacttcagc
     1621 atgcgctatc cgctggtcga cggacagggc aacttcggct ccatcgacgg cgatcctgcg
     1681 gcggcaatgc gatacaccga ggccaaactc ttcgaaacag gagagctgat gctggcggat
     1741 atcgacgaag acactgtgga atgggggccg aacttcgacg aatcgctcaa ggagccgttg
     1801 accctgccct cgatgatccc gaatctcctg gtgaacggaa gttccgggat cgctgtgggc
     1861 atggcgacga acattccccc gcataacctg tgcgaagttg tggatgcact cgaatacctg
     1921 attgaagccg acggaaatat tgagttcggc gagatttacg cgcggctgcc cggccctgat
     1981 ttcccgaccg gcggagtgat tctcggacgc aatggcatta tcgacgcgta ccgcaccggg
     2041 cggggaaaga tcatcctccg cggcaaaacg catgtggagg aggggaagaa aggcaagacc
     2101 tcgatcgtca tcaccgaaat cccctttatg gtgaacaaaa cgctgttgat cgagacgatc
     2161 gcctcctgcg tgcaggaaaa gcacatagac ggcatttccg acatccggga cgagtcggac
     2221 cgtgacggac ttcgcgtcgt tctcgaactg cagcgcgacg ccgacgcgga acttgtgctc
     2281 cgccagcttt accgccgcac acagctccag tcgacattcg gagtcatcaa tctcgcgctg
     2341 gtggagaaca gtccgaaaga gctccccatc accgacatgc tctctctctt cctcgaacac
     2401 cgccgcgacg tcgtccgcag acggacgcag ttccgtctcg acaaggccct cgcccgtgcg
     2461 catatcgtcg aagggctcgt caaagcgctt gatatcatcg accaggtcat cgcgaccatt
     2521 cgggcggcgc agacgacgca ggaagcaagg gaagggctgg tcgacaccct cggattttcg
     2581 gagcttcagg cgcaggcgat actcgaaatg cgcctgcagc gcctcacagg ccttgaaagg
     2641 gaaaagctcg acgctgagct ggccgcgctt ctcgccgata tcgaacgctt ccggagcatt
     2701 cttggcaatg cgaaaattct ggacggcgtg atcaaagacg aactgagtct tctgagaaag
     2761 aagttcggca gccccaggag gacggagata atcgacagct acgaggaggt gtccatagaa
     2821 gatctcatcc cggaggaaga tatcgtcgtg attctctcca aggacggcta tctccgccgg
     2881 aagagcctcg acgagtacgt cccgcaggcg aggggcggaa agggccggaa aggcgccgcg
     2941 ctccaggacg aggatgagat cgccgttttt gcggtgacga acacccacaa ggacatttat
     3001 ttgtttacat cctccgggcg tgtgctcgcg atacgggggc acatgattcc ggaatcacgc
     3061 tccggcaagg gcaaactcgt cgggaagctt gtgccgctgg aggacggaga gacggtcgtc
     3121 tccatgtacg gacggagctt ggaggggaag agctatctct tcttcgcgac gaaaaacggc
     3181 atagcgaaac gtctgcaact ttcggagatt ttgcgcatta acaggggagg gaaacgagtg
     3241 cttacccttg atgaaggcga cgaaatcgcg caggtgcgcc tgacgtccgg ggatgacgaa
     3301 ctgctcttta tgaccgctgc tggacaggct ctccgcgttc ccgaacaaga gttccgcgca
     3361 atggggaggg ccgcgcgcgg cgtcaagggc atacgccttg caaaggacga ctccgtaata
     3421 agttgtgaag tcgtgaataa agagcggcat gcgcttgtca tcagtgaaaa gggggtagcc
     3481 aagaggacga gctttgagga gtttaccctc catcacaggg gcggatccgg cgtgaaggcg
     3541 atgaacctcg gaagaaagac cgggatgctt gtgggcagct ggtctgtggc ggaagaagac
     3601 gaaattatgg cgatcacggc gcgaggcaga attatccgcg tcgccgtttc ggagatccct
     3661 gttttaagca gaacggctat gggctccatc actgttcgcc tcgacggggg ggacagcgta
     3721 gcggacgtga gcgttgtctg cggagaggtt tgcgttgccg cggagatccc atacgaggag
     3781 gagactgaat gaaactggct cgagaaggct acccgcttat cgcattcaca gtgttcatga
     3841 ttatcgggag cgttttcttc gcccctcccg tggcgctctt tcttctccct gtccttggcc
     3901 tcgtcgtctg gttttttcgc gaccctgaac gattcccgga tggcgaggga tttctctctc
     3961 ccgcggacgg gcgtatagtg gaaatcgagc aggcggacct tcccttcaca ggaaaggcgt
     4021 ggaagatcgg tatttttatg aacccgctga gcgtgcacgt caaccgtttc ccctgcgccg
     4081 gtactgtgga gcatcttgag tacgtcccgg ggaagaaatg gatggcgttc gcccccaagg
     4141 cttcggaagt caacgagcgg atgctcgtcg gtatttccac ggagtacggt cccgtcctcc
     4201 tggtgcagat cgccgggttt ctggcgcgcc gcatcgtctg ccgtctccgc aagggagacc
     4261 ggctcgaaag agggcagcgg tacgggatga tcaagatggg gtcgaaagtc gatgtctatt
     4321 tgcctgcgaa gactcgcctt gccgttgcaa aggggcagca ggtctttgca ggacgtacgg
     4381 ttctgggagg tgtgtcctgt ggcgagtagc atcagaaaga aacggcgtcc tctccctttc
     4441 cgcgagatag cgcccaacat ggtcacgagc ggcaatctgc tctgcgggac gatgtcgctc
     4501 attcttgtgc ttcacggcag agtggtccct gcggcgtggc tcgttttcat ggcggttttc
     4561 ttcgacttca tggacggaaa ggttgcgagg cggctcggcg gcgggacgcg cttcggtctt
     4621 gaattcgaca gcctggcaga cgttgtgagc ttcggcgttg ctccggccgt tcttttttac
     4681 tcggtgtatg cggagcggct cggcgtcatg ggcgcgcttg ccgtctcctt ctttgcgctt
     4741 tgcggcgccc tgcgtctcgc gcgattcaac gtcacacacg tgcccgggcc cttccagggg
     4801 ctgcctattc cagcaggcgg gctttttctg gcctcttttg ttctcgccgg agctcatctc
     4861 tctccatggc tcgcggcgtt gcttgcgctc ggaacaggcg cgctgatggt gtcgagcgtc
     4921 ccctacagca gcatgaagaa gctgcgcagg gggtacgcgc gaaagaagcg gcttctcctg
     4981 gtggggggcg ccgcgatcag cggagcgatc ctgctggaga gcgcggcgcc tcttgccgga
     5041 atttcgcttt atgtcgtcag cggtatactg cgttttgact gggaaaagtg gctttccgtt
     5101 cccggagcag aggaggagcg gatcgaaaag agcctttgag tgtctttttt ctttttttac
     5161 aatcgatagt gacgggggag cccgaacggg ctgagagg
//
LOCUS       DAOTIL010000074         5189 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminobacteriaceae bacterium isolate AMR_MDS_3026
            NODE_18953_length_5189_cov_4.362680, whole genome shotgun sequence.
ACCESSION   DAOTIL010000074 DAOTIL010000000
VERSION     DAOTIL010000074.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868050
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminobacteriaceae bacterium (wastewater metagenome)
  ORGANISM  Aminobacteriaceae bacterium
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae.
REFERENCE   1  (bases 1 to 5189)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5189)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:19:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,224
            CDSs (total)                      :: 3,198
            Genes (coding)                    :: 3,184
            CDSs (with protein)               :: 3,184
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5189
                     /organism="Aminobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18953_length_5189_cov_4.362680"
                     /isolate="AMR_MDS_3026"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:3034609"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1035)
                     /locus_tag="P5201_02515"
     CDS             complement(<1..1035)
                     /locus_tag="P5201_02515"
                     /EC_number="2.4.1.129"
                     /inference="COORDINATES: protein motif:HMM:NF013279.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008955 - peptidoglycan
                     glycosyltransferase activity [Evidence IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsW/RodA/SpoVE family cell cycle protein"
                     /protein_id="HRV97436.1"
                     /translation="MEEKKLSLGESVAGLDKAQLFSVGALFLLGVLSIFSAGAGFEGR
                     GAGFATRQLLWGAVSIAAFLLTLKIGYEKFLREAYLLYGLSLVLLLSVMALGLTVKGS
                     QRWIDLGFFRLQPTEFVKITLVLVLAKHLCRYPPLNFLSFLGGLAVPALSGILVLIQP
                     DAGSAIVYGVISFVAIVVAGAPWRYPAGLLAIFTSLLPFAWRFLREYQKMRIRVFLDP
                     WIDPLGAGYNVIQSRIAVGSGGLFGAGYMEGLQSKLKFLPEPHTDFIFGVFSEEFGFI
                     GALGMLALFGLLFWRLLDAGLRTKDGRAKVLIAGISTWFWFQMLQSIGMSMGLLPITG
                     LPLPLVSYGGS"
     gene            complement(1099..1383)
                     /gene="minE"
                     /locus_tag="P5201_02520"
     CDS             complement(1099..1383)
                     /gene="minE"
                     /locus_tag="P5201_02520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009201507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA];
                     GO_process: GO:0000918 - division septum site selection
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division topological specificity factor
                     MinE"
                     /protein_id="HRV97437.1"
                     /translation="MDFLKKFFSSSGSKSRAKERLQIVLIHDRADISPEMLDNLRQDM
                     IAVITRYMDIDTSKIEMDFDRADRSVAFVANIPVIRIKRGVAAAREGERG"
     gene            complement(1403..2209)
                     /gene="minD"
                     /locus_tag="P5201_02525"
     CDS             complement(1403..2209)
                     /gene="minD"
                     /locus_tag="P5201_02525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014162779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0000918 - division septum site selection
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="septum site-determining protein MinD"
                     /protein_id="HRV97438.1"
                     /translation="MDSRVIVITSGKGGVGKTTTTSNLSVALARGGHRVVAVDADIGL
                     RNLDVVMGLENRVVYNFIDVLEGNCRLNQALVRDKRVDNLFLLPAAQTRTKDAVSSEQ
                     MEELCNLLRQEFEYILIDSPAGIESGFRNAAVGADEALVIATPEVPAVRDADRIIGLL
                     ESMGKAPIRLIVNRLRAQMVRDGEMLDVSDVLDILAIELIGVVPEDDSVVKSANRGEP
                     LTFSDVSPAALAFQRIAMRLTGKEVPFPKLDEENGRGLFAGIRKLFGMKN"
     gene            complement(2263..2976)
                     /locus_tag="P5201_02530"
     CDS             complement(2263..2976)
                     /locus_tag="P5201_02530"
                     /inference="COORDINATES: protein motif:HMM:NF015716.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000902 - cell morphogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="septum site-determining protein MinC"
                     /protein_id="HRV97439.1"
                     /translation="MAGTVESLENPKDTAEHRGTSEILLKGTGYGVRMILPETKTDED
                     LLQEALAFSGEAARLTRGIGVVLDFQGRTISRAFLLRFLSEFVWTGAFRVLSWMSYNA
                     DTLELFRASGFSTGEPTAARSEKKESGGATLLLIQSLRSGQRVEHEGDVVLLGHMNEG
                     AEICASGNVFVRGRLKGVVHAGMNGGEYSISAGQFEARQLRLGGKLCSSLGTEMEWWG
                     KAVVISLENDSLVVRELKL"
     gene            complement(3079..4824)
                     /gene="mrdA"
                     /locus_tag="P5201_02535"
     CDS             complement(3079..4824)
                     /gene="mrdA"
                     /locus_tag="P5201_02535"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013048005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein 2"
                     /protein_id="HRV97440.1"
                     /translation="MSDHRPEMNLRLLLWKLFLMFSLLLLAGGLYFFQIVNADAYVRL
                     AANNRLRFIRFAPIRGEIFDRNGVPLAANVTTFDIMGYPLDIEKNNMVENLALLLSRH
                     GIPVSAEDLRRDVKRQFWAPYRVVRVVSNITLAQMADLVADPEFPRQLFPIPVLRRTY
                     PAGSIASNVTGFVGEINENELRQMEGKSYVGGDQIGKTGIERQYEEILRGEPGGESVE
                     VDARGRRVRQIDTYPAGRGEDLHLTLDLGAQRLAADLLQGYRGALIVMDVHDGDVLVL
                     ASSPSYDNNPLSWGVSAKEWNALLSDPQKPMLDRSIAGLYPPASTFKILVGLAALVEK
                     EVTPSTTFHCGGAFTLGNRTFRCWRRSGHGTVNLTGALQNSCDVYFYQVGLKLGITRL
                     LKWVELFGLGRPTGIDLPGEAGGVVAGPDWKKARFKEGWYRGDTVNYSIGQGFLLTTP
                     LQLATMYAVIANGGKLVRPRLANSLPVQAAELNLSPSHMNVIRKGLDYVVRRGTARRA
                     GGFGITVAGKTGTAQNAHGDDHALFVGYAPMDNPKYVAVAMLEAGLHGGSVASPMVGE
                     MLSYLLVPESRMKTP"
     gene            complement(4817..>5189)
                     /locus_tag="P5201_02540"
     CDS             complement(4817..>5189)
                     /locus_tag="P5201_02540"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRV97441.1"
                     /translation="TLVFLSLFRSEDGVRVVWAAFLGGVLWDLRWTGFPGISSLIYVL
                     SLMSVRWIWHSIPASGRTITLFGFLLWSANMPIALGRIFLWGARGIEVWRAYLLQQSH
                     IVPLVLLACAAYAWRLKNQDV"
BASE COUNT         1130 a         1676 c         1411 g          972 t
ORIGIN      
        1 actgcccccg taactgacga gcggaagggg cagccccgtt atgggcagga gccccatgct
       61 catgccgatg ctttgcagca tctgaaacca gaaccaggtt gaaatcccgg cgataaggac
      121 cttggccctc ccatccttgg tgcgcaatcc ggcgtccagg agccgccaga acagaaggcc
      181 gaagagggcg agcattccca gagcgccgat aaagccgaac tcctccgaga agacgccgaa
      241 gatgaagtcg gtgtgcggct cgggcaaaaa cttgagtttg ctttgcagcc cctccatata
      301 gccggcgccg aacaaccccc ccgagccgac ggctatccgg gactggatga cgttgtaccc
      361 ggcgccgagc gggtcgatcc acggatcaag aaagacgcgt atccgcattt tctgatactc
      421 ccgcaaaaaa cgccatgcga acggaagaag cgacgtgaag atggccaaca gccccgccgg
      481 atacctccat ggggctccgg cgacgacaat cgcaacaaag gagatcacgc cgtagacgat
      541 cgcgcttccg gcatccggtt gaatcagcac caggatcccc gaaagcgcgg gcacggcgag
      601 cccccccaga aaagagagaa agttcaacgg ggggtacctg cacagatgtt tggcaagcac
      661 cagaaccagc gttatcttga cgaactccgt cggttgcaac ctgaagaaac ccaggtcaat
      721 ccaccgctgc gaccccttca cggtgagccc cagcgccatc acgctcaaca agagcaccag
      781 cgaaaggccg tacaggagat acgcctcccg cagaaatttt tcgtacccta tcttgagagt
      841 gagcaaaaaa gccgcaatcg acactgcgcc ccagaggagc tggcgagtcg caaaccccgc
      901 tccccgcccc tcaaaaccgg cgccggcgct gaatatggag agcaccccga gcagaaagag
      961 cgcgcctacg gaaaaaagct gcgccttgtc caggcctgcg acactctcgc caagagaaag
     1021 ttttttttct tccattacaa gttctccctc gtacgcggcg tctccgcttc cgacggcgtt
     1081 ctcctccgcg gggacacgct agccccgttc tccctctctc gccgccgcaa cccctcgttt
     1141 gatgcggatc acagggatgt tcgccacgaa cgccacggat ctgtccgcac ggtcgaagtc
     1201 catctctatc ttcgatgtat cgatgtccat atacctcgtg atcacggcaa tcatatcctg
     1261 ccggagatta tcgagcatct cgggagagat atccgcccgg tcgtgaataa gcacgatctg
     1321 gagacgctct tttgcccttg atttcgaccc ggaagagctg aaaaatttct tgagaaaatc
     1381 catcgccacc cctcctctcc tctcagttct tcatcccgaa gagcttgcgg ataccggcga
     1441 aaagtcccct gccgttttcc tcgtcgagtt tcggaaaagg gacctctttc cccgtcagac
     1501 gcatggcgat cctctgaaat gcgagggcgg ccggagagac gtcggagaag gtaagaggct
     1561 cgccgcggtt ggccgacttc acgacgctgt cgtcctcggg aacaacaccg ataagctcga
     1621 tcgcgagtat gtcgagtacg tcggaaacgt cgagcatctc gccgtcgcgg accatctgcg
     1681 cgcggaggcg gttgacgatc agacggatcg gcgcttttcc catcgattca agaaggccga
     1741 tgatcctgtc ggcgtcacgc acagccggca cctccggcgt ggcgataaca agcgcctcgt
     1801 ccgcgcccac ggcagcgttc cggaaaccgc tctcgatccc cgccggactg tcgatcaaga
     1861 tatactcgaa ctcctgccgg agaaggttgc acagctcctc catctgttcg gaggagacgg
     1921 cgtcttttgt ccgggtctgc gcggcgggga gcagaaaaag gttgtccacg cgcttgtctc
     1981 tgacgagcgc ctggttcaac ctgcagttcc cctcgagaac gtcgatgaag ttgtagacca
     2041 ccctgttctc gagccccatc accacgtcga ggttcctgag cccgatatcg gcgtcgacag
     2101 ctacgacgcg gtgaccgccg cgcgcgagcg ccaccgaaag attcgatgta gtggttgtct
     2161 ttcccacccc tccctttccg gaggtaatca cgataacacg agaatccaca gcaagatctc
     2221 tccttccctc ccgatcgccc tttcagagcg atattcgggg gcttataact tcagttccct
     2281 cacgacgaga gaatcgtttt ccagactgat cacgacggcc tttccccacc attccatttc
     2341 cgtgccgagg gaggagcaga gcttccctcc gaggcgcagc tgcctggctt cgaactggcc
     2401 cgccgaaata ctgtactcgc cgccgttcat cccggcgtgg acgacgccct tgagcctgcc
     2461 cctgacaaag acgtttcccg aggcgcatat ctccgcccct tcgttcatat gtccgaggag
     2521 caccacgtcg ccctcgtgct ccactctctg tccggaacgc aaagactgta tgaggagaag
     2581 cgtggcgcct ccgctctcct ttttttcact cctcgcggca gtcggttcgc cggtggaaaa
     2641 accggatgcg cgaaagagtt caagcgtatc cgcattgtag gacatccacg aaagaacgcg
     2701 gaaagccccc gtccagacaa attccgaaag gaaacggagc aggaaagccc gggagatcgt
     2761 tctcccctga aagtcgagaa caacgccgat gccgcgggtc agacgggcag cctctcccga
     2821 gaaggcaagc gcctcttgca gaagatcttc gtccgtcttc gtctccggga gtatcattcg
     2881 gactccgtac cccgttcctt tcaggagaat ctcggacgtc ccgcggtgtt cggcagtatc
     2941 cttcggattt tcaagagatt ccactgttcc agccacagta ccccctcctt cggtaaaaag
     3001 ctcgcttctc cattttccac ctctgcggta tgttcggcgg gaaataccga actccccggg
     3061 cggcttccat catctccttt acggagtttt catcctggat tcgggaacga ggagatacga
     3121 gagcatctcc cccaccatgg gcgaggccac ggaacctccg tggagcccgg cctccagcat
     3181 ggccacagcg acatatttcg ggttatccat cggcgcgtac cctacgaaga gcgcgtgatc
     3241 atccccgtgc gcgttctgag cggttccggt ctttcccgcg accgtaatgc cgaatcctcc
     3301 cgccctccgg gccgtccctc tccggacgac atagtccagc cccttgcgta tgacgttcat
     3361 atggctcggt gaaaggttca gttcagccgc ctgcaccggc aaactgttcg cgagccgcgg
     3421 ccggacaagc ttgcctccgt tggcgatcac agcgtacatt gtggcgagct ggagcggcgt
     3481 cgtcagcaaa aacccctgcc caatggagta attgacggta tcgcctctgt accagccctc
     3541 cttgaaacgg gccttcttcc agtcggggcc ggccacgacg ccaccggcct ctccgggaag
     3601 atcgattcct gtgggcctcc ccaatccgaa aagctcaacc cacttcaaaa ggcgggtaat
     3661 cccgagcttc agtccaacct ggtaaaaata gacgtcgcag gaattctgaa gcgcccccgt
     3721 caggttcacc gtcccgtgtc ccgaacgtct ccagcagcga aacgtacggt ttcccagggt
     3781 gaaagcgccc ccgcagtgga aggtcgtcga cggagtgacc tccttctcta caagagcggc
     3841 aagcccgacg agtatcttga aggtcgacgc cggcgggtaa agcccggcga tactcctgtc
     3901 gagcatcggc ttctgcgggt cggagaggag cgcattccac tccttcgccg aaacgcccca
     3961 cgagaggggg ttgttgtcgt acgaagggga agaggccagt acaaggacgt ccccgtcgtg
     4021 cacgtccatc acgatcagcg ctccgcggta tccctggaga agatcggccg ccaggcgctg
     4081 cgccccgaga tcgagcgtca ggtgaaggtc ctcgccgcgc cctgccggat aggtatctat
     4141 ctgccggacc ctgcgccccc tggcgtccac ctcgacggat tctcctccgg gctccccgcg
     4201 aaggatctcc tcgtattgcc tctcaatacc ggtcttcccg atctggtctc ccccgacgta
     4261 gctcttcccc tccatctgac gcagctcgtt ctcgttgatc tcgccgacaa atcctgtgac
     4321 gttcgacgcg atacttcccg ccggataggt ccttcgcagg acggggatcg gaaagagctg
     4381 ccgcgggaac tcgggatcgg ccaccaggtc ggccatctgc gcgagggtta tgttggagac
     4441 cacgcgcacc accctgtagg gcgcccagaa ctgccgcttg acatcccggc gaaggtcctc
     4501 cgccgatacc ggaataccgt ggcgggagag caaaagcgca agattctcca ccatgttgtt
     4561 cttttcgata tcaagcgggt accccattat atcgaatgta gtcacgttcg ccgccagcgg
     4621 gactccgttc ctgtcgaaaa tctctccccg tatgggggcg aaccggatga accggaggcg
     4681 gttgttcgcc gcgaggcgaa cgtacgcgtc ggcgttgacg atttgaaaaa agtaaagccc
     4741 tcccgcgagc agaaggagcg aaaacataag aaacagcttc cacaaaagaa ggcgaagatt
     4801 catctccgga cgatggtcag acatcctggt ttttcaacct ccacgcatag gcggcgcagg
     4861 caaggagcac caacggcaca atgtgactct gctgaagcag gtacgcccgc cacacttcta
     4921 ttccccgcgc tccccacagg aaaattcttc cgagcgcgat aggcatgttc gcggaccaga
     4981 ggaggaaacc gaagagcgtg atcgtccttc ccgaggccgg gatggaatgc caaatccacc
     5041 ggacggacat caatgacaga acgtaaatca aggagctgat acccggaaat ccggtccatc
     5101 gtaaatccca gaggacgcct ccgaggaaag cggcccaaac cacccgaact ccgtcctcgg
     5161 aacgaaagag cgaaagaaag acgagggtg
//
LOCUS       DAOTIL010000075         5172 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminobacteriaceae bacterium isolate AMR_MDS_3026
            NODE_19035_length_5172_cov_2.950362, whole genome shotgun sequence.
ACCESSION   DAOTIL010000075 DAOTIL010000000
VERSION     DAOTIL010000075.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868050
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminobacteriaceae bacterium (wastewater metagenome)
  ORGANISM  Aminobacteriaceae bacterium
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae.
REFERENCE   1  (bases 1 to 5172)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5172)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:19:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,224
            CDSs (total)                      :: 3,198
            Genes (coding)                    :: 3,184
            CDSs (with protein)               :: 3,184
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5172
                     /organism="Aminobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19035_length_5172_cov_2.950362"
                     /isolate="AMR_MDS_3026"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:3034609"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1146)
                     /locus_tag="P5201_02545"
     CDS             complement(<1..1146)
                     /locus_tag="P5201_02545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009164871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5'-3' exonuclease H3TH domain-containing
                     protein"
                     /protein_id="HRV97442.1"
                     /translation="MSAPDRKKLLLVDGHGLAFRAFYAVRELNAPDGTPTNAIVGFFN
                     MFQKIREDWNPDLCGVVFDAPGPTFRHEAYAEYKAGRKPTPPEFKTQLPIILELLDAM
                     GVPVVSREGVEADDVLASSACAAAAQNLETLILSSDKDILQVLDRDVVVLRPVTGISS
                     FRTMDGKGFEEEYGFSPAAMPDYLALLGDSVDNVPGVPGVGEKTAKKLLSDYGTLEGV
                     YERLDELKGALRKKMEEHREQAFRSRELTRLLCDTEEDLSVFEPRPVEAKRLLEKCRV
                     LGLKSLAAKFQLAETAEEAHEHKKDIRLCEVSAEEIAASESVLLLPVIEGKYPLHLSA
                     SELLVLDETGGYRRFPGAEAAPLLGRLGELSKEAVLFPDFKEAAACFG"
     gene            1287..2603
                     /gene="gltX"
                     /locus_tag="P5201_02550"
     CDS             1287..2603
                     /gene="gltX"
                     /locus_tag="P5201_02550"
                     /EC_number="6.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008522729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009332 - glutamate-tRNA ligase complex
                     [Evidence IEA];
                     GO_function: GO:0004818 - glutamate-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--tRNA ligase"
                     /protein_id="HRV97443.1"
                     /translation="MADTVRVRFAPSPTGALHIGGGHTALFNWLWARHTGGKFILRIE
                     DTDRERSTKEYEDTIMSGMQWLGLDWDEGPDTGGPFGPYRQSERLSTYREAAERLLDE
                     GKAYRDGEAVIFKVPQGVTLAFDDMVYGHIEMQSDTLKDVVMIKSDGMPTYNYAVVID
                     DHTMGITHVIRGEDHISNTPKQLLIYDALGWEYPTFAHLPMILGKDKKKLSKRHGATS
                     VYEYRDMGYMPDSVFNFLALLGWSAGENNEIFSRGEAVGLFDLARVIKKPPVFDMDKL
                     NSINQEHLKRLPPYERLALVEPFWREMGLPVDSHSREYLAQSLVVLCGRGQTAKEAAG
                     YSDYFLSFDPVKARYDGSEIGDEQRAVLKRFNGELLALPEWKAPLMEEFTREWSTANN
                     VKMKDIAMPLRLGITGMKVSPGVFEVAEQLGRDEVRKRLAYYNFVE"
     gene            complement(2694..3314)
                     /locus_tag="P5201_02555"
     CDS             complement(2694..3314)
                     /locus_tag="P5201_02555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013048646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HRV97444.1"
                     /translation="MITRMLFQLLRQIRQKVFTNPFPVAQMPDSLTDALQAAEKGWIE
                     LNPPVGVNDHFRGRLNYDKSACIGCKLCVKVCPANATEYLPEEKKIQIHVDRCCFCAQ
                     CVEICPVKCLTMSGEYLISSYDRKAQIVTDTGKPEGAPVAPAAKTVYRIDPEKCIGCT
                     KCARNCPVPCIEGKVKEKHVIDESKCIGCGKCAEVCPKDAVSPVEV"
     gene            complement(3331..4323)
                     /locus_tag="P5201_02560"
     CDS             complement(3331..4323)
                     /locus_tag="P5201_02560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005662522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit H"
                     /protein_id="HRV97445.1"
                     /translation="MIALLMRLVAGTALMLLVVVLALLFDGADRIISARMQRRLGPPL
                     LQPFLDLLKLLGKENIVPRRAVPWVFNGAPWVAAASMLLVFLYIPVGSVPPVLGVHGD
                     LILILYLLGLSGVAMAVGGFASGNPIANVGAQREMILMMSYELPLAVVASTLAWVAYK
                     TGMPGDPFNLETFAGMSVWGMSGKIGFIGIFCLMVALILVVPGETGKGPMDIPEAKTE
                     ILDGLIIEYSGRNLAMFKITFALRAMALSALITALFFPWSPASFLRLGGIFLFTFDFV
                     WFWVKVFVVQIFVVTLMRTTFGRFKIWQASEFYLVKVAGLSIAGMILASLGLVV"
     gene            complement(4320..>5172)
                     /locus_tag="P5201_02565"
     CDS             complement(4320..>5172)
                     /locus_tag="P5201_02565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014807277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="NADH dehydrogenase subunit"
                     /protein_id="HRV97446.1"
                     /translation="FHLGMVLREKVMDVLEALSGNRVNYGVATVGGVRWDITPEVAKA
                     VREMVKYYKAEFGAFYDVVTADPIAKARMRNVGVLTPDEAVRYNAVGPTARASGIRCD
                     IREMSPYEAYADFNVKAIVPQDYYGKAYGDVFDRFLVRVLEVQQSLNIVERVMDGLPE
                     GEIVHEKNLNKVLATLKKAEGTGWGVIEAPRGDDTHVVHLKAGEENVNWWKVRAPTYA
                     NAVSWPLMFRDNELADAPLIINSIDPCISCMERMLVTDAAGGRSVVTKAELLEKSREK
                     TRRLMQK"
BASE COUNT         1038 a         1633 c         1528 g          973 t
ORIGIN      
        1 gccgaaacag gcggcggcct ccttaaagtc cgggaagagc acggcctcct tcgagagttc
       61 gccaaggcgg ccgagaaggg gcgccgcttc agctccgggg aaacgccgat agccccctgt
      121 ttcgtcgagc acgagaagtt ccgaggccga aagatgcagc gggtacttcc cctcgatcac
      181 agggaggaga agcacactct ccgaagccgc tatttcttcc gccgacacct cgcagagccg
      241 gatgtccttc ttgtgttcat gcgcttcttc cgccgtctcc gccaactgga actttgcggc
      301 aaggctcttc agcccgagga cgcggcactt ctccaataac cgcttcgcct ccacagggcg
      361 aggttcgaat acggagagat cctcctcggt atcgcagagg aggcgcgtca gctcccggct
      421 ccggaacgcc tgctccctgt gctcctccat ctttttccgg agcgctccct tcagttcatc
      481 caggcgctcg tacacgcctt cgagcgttcc gtagtccgaa agaagcttct tcgccgtctt
      541 ctcgcccacg ccgggaacgc cgggtacgtt gtcgacggaa tctccgagga gcgcaaggta
      601 gtcgggcatc gccgcagggg aaaagccgta ctcctcttcg aagcccttcc cgtccatcgt
      661 acgaaaggaa ctgatgcccg tcaccggcct gaggacgacg acgtccctgt cgagcacctg
      721 gaggatgtcc ttgtcggacg agaggatcag cgtctccagg ttctgcgccg cagcggcgca
      781 cgcgcttgaa gcaagcacgt cgtccgcctc cacgccttca cgcgacacca ctggaacgcc
      841 catggcgtcg agcagctcaa gaatgatcgg gagctgcgtt ttgaactcgg gcggcgtcgg
      901 ctttcgcccc gccttgtact ccgcgtacgc ctcgtggcgg aaggtcggcc cgggggcgtc
      961 gaagacaacg ccgcagaggt cggggttcca gtcttccctg atcttctgga acatattgaa
     1021 gaaaccgacg atcgcgttcg tcggcgttcc gtccggcgcg ttcagttcgc ggacggcgta
     1081 gaaagctctg aaagcaagcc cgtgtccgtc cacgagaagt agtttctttc tgtccggtgc
     1141 gctcatatcg ttccgccttc ctttttcatc caactggttt tttctgcgtt caactaggtt
     1201 ataataacct attgcaggaa ttcatgcaca cacaatgcac ggtcccgaat cagcaggcgg
     1261 agggacgtca tcggggagga actgaaatgg cagacacggt acgggttcgc ttcgcgccga
     1321 gcccaacggg agcgcttcat atcggcggcg ggcacacggc cctgttcaac tggctgtggg
     1381 cgcggcacac gggagggaag ttcatcctcc gcatcgagga tacggacagg gagcgctcca
     1441 caaaggagta cgaggacacc atcatgtcgg gcatgcagtg gctcgggctc gactgggacg
     1501 aaggaccgga cacgggcggt ccgttcgggc cgtacagaca gtccgaacgc ctctcgacct
     1561 accgcgaggc ggcggagcgg cttcttgacg aggggaaagc ctaccgcgac ggagaggccg
     1621 tcattttcaa ggtccctcag ggcgtcacgc tcgccttcga cgacatggtc tacggccata
     1681 tcgagatgca gagcgacacg ctgaaggacg ttgtcatgat caagagcgac ggcatgccca
     1741 cctacaacta cgccgtcgtc atcgacgacc acacgatggg gatcacgcac gtcatccggg
     1801 gcgaggacca tatttccaac acgccgaagc agctcttgat ctacgacgcg ctcggctggg
     1861 agtacccgac ctttgcgcac cttccgatga tcctgggcaa ggacaagaag aagctctcca
     1921 agcggcacgg ggccacaagc gtttacgagt accgggacat ggggtacatg cccgactcgg
     1981 tcttcaactt tctcgcgctg ctcggctggt cggccggcga gaacaacgag atcttctccc
     2041 gcggcgaggc cgtggggctc ttcgacctcg cacgcgttat caagaagccg cccgtgttcg
     2101 acatggacaa gctgaactcc atcaaccagg agcatctgaa gcgccttccg ccgtacgagc
     2161 gacttgcgct cgtcgagccc ttctggaggg agatggggct tcccgtggat tcgcactcca
     2221 gggagtacct cgcccagtcg ctcgtagtgc tctgcggcag agggcagacg gcaaaggaag
     2281 ccgcgggcta ctccgactac ttcctgtcct tcgaccccgt caaagcgagg tacgacggct
     2341 cggagatcgg cgacgaacag cgcgccgtcc tcaaacgctt caacggcgag ctgctggccc
     2401 tgcctgagtg gaaggccccg ctcatggagg agttcacacg ggagtggagc acagccaaca
     2461 acgtgaagat gaaggacatc gccatgccgc ttcgcctggg catcaccggg atgaaggtaa
     2521 gccccggtgt cttcgaggta gcggagcagc tcgggcgcga cgaagtgcga aaacgtctcg
     2581 cctactacaa cttcgtcgag taaccgcaca cgcgggaaag tacacagagc gaaggcggac
     2641 ctccgtaaag agaggcccgc cttcgctctg ttcgttggtt tactgctccg ccgttacacc
     2701 tccacggggc tgacggcgtc cttcgggcag acttcggcgc acttgccgca gccgatgcac
     2761 ttcgattcgt cgataacgtg cttctccttc accttcccct cgatgcaggg gacggggcag
     2821 ttccgggcgc acttcgtaca gccgatgcac ttttcggggt cgatccggta gacggtcttc
     2881 gccgcagggg cgacgggagc gccctccggt tttcccgtat cggtgacgat ctgggccttc
     2941 ctgtcatacg acgagatcag atactcgccg gacatggtga ggcacttgac gggacatatt
     3001 tcgacgcact gggcgcagaa gcagcatctg tcgacgtgga tctgaatctt cttctcctcg
     3061 ggcagatact ccgtagcatt cgccgggcaa accttgacac agagcttgca ccctatgcat
     3121 gcgctcttgt cgtagttcag gcgtccccgg aagtgatcgt tcacaccaac gggtgggttc
     3181 agttcgatcc accctttttc ggcggcctgc agcgcgtcgg tcagcgagtc gggcatctgc
     3241 gccacgggga aggggttcgt gaacaccttc tggcgaatct gccgcagcag ttgaaagagc
     3301 attctggtga tcattccgtt ccaccccttc ctatacgacc aggccgaggg acgcgaggat
     3361 catccccgcg atggaaagcc ccgcgacctt gacgagatag aactcggacg cctgccagat
     3421 tttgaacctg ccgaacgtcg tccgcatcag cgtcaccacg aagatctgca ccacgaagac
     3481 cttcacccag aaccagacga agtcgaatgt aaagaggaag atgcctccca ggcgaaggaa
     3541 ggatgcggga gaccacggga agaagagcgc cgtgatcagt gcggagagcg ccatggcgcg
     3601 cagcgcgaag gtgattttga acatggcgag gtttcttccg gagtactcga tgataagacc
     3661 gtcgagaatc tccgtcttcg cttcggggat gtccatcggc cccttcccgg tctctccggg
     3721 aacaacgagg ataagagcta ccatgagaca aaaaataccg atgaagccta tcttgccgct
     3781 cattccccag accgacatcc cggcaaaggt ctccaggttg aacggatctc cgggcattcc
     3841 cgtcttgtag gcaacccacg caagcgtgga ggcaacgacg gcgagcggaa gttcgtagct
     3901 catcatcagg atcatttcac gctgcgcgcc cacattcgct atcgggtttc cgctggcgaa
     3961 gccgcccact gccattgcaa cgccggagag ccccaggaga tagagaatca ggatcaaatc
     4021 tccgtgcacc ccgagcacgg gaggcaccga gccgaccggg atgtagagga aaacgaggag
     4081 catgctcgct gccgcaaccc agggcgcgcc gttgaaaacc cacggaactg ccctccgcgg
     4141 cacgatgttc tccttgccga ggagcttcag caggtcgaga aagggctgca gaagaggcgg
     4201 acccaggcgg cgctgcatgc gggcgctgat gattctgtcc gctccgtcga aaagaagcgc
     4261 cagcacgacg acaagaagca taagcgctgt accggcaacg aggcgcataa gaagcgcgat
     4321 catttctgca tcagcctcct tgttttttcc ctgctctttt ccaggagctc cgccttggtg
     4381 acgacgctcc tgccgccggc ggcgtccgtg acgagcatac gctccataca cgagatgcag
     4441 gggtcgatgc tgttgataat aagcggcgcg tcggccagct cgttgtcgcg aaacatcaac
     4501 ggccatgaaa cggcgttcgc gtacgtcggg gcgcgcacct tccaccagtt cacgttctcc
     4561 tcgccggctt tcagatgcac cacgtgcgtg tcgtcgcccc tcggagcctc gatgacgccc
     4621 caccccgtgc cttcggcctt cttgagcgtc gcgagcacct tgttcaggtt cttctcgtgg
     4681 acgatctcgc cctcgggaag accgtccatc acccgctcga cgatattcag cgactgctga
     4741 acctccagca cgcgtacaag gaaacggtcg aacacgtcgc cgtatgcctt gccgtagtag
     4801 tcctgcggaa cgatcgcctt cacgttgaaa tctgcgtacg cctcgtaggg agacatctcg
     4861 cggatatcgc agcggatgcc gctcgcgcgg gccgtcggcc ccaccgcgtt gtaccggaca
     4921 gcctcgtccg gcgtgagcac gccgacgttg cgcatgcgcg ccttagcgat aggatccgcc
     4981 gtgacgacat cgtagaacgc gccgaattcc gccttgtagt atttcaccat ttccctgacg
     5041 gccttcgcta cctcgggcgt gatatcccac cgcacgccgc cgactgtggc gacgccgtag
     5101 ttcaccctgt ttccggagag cgcctcaagc acgtccatga ctttctcgcg gagcaccatc
     5161 ccaaggtgga ag
//
LOCUS       DAOTIL010000076         5163 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminobacteriaceae bacterium isolate AMR_MDS_3026
            NODE_19073_length_5163_cov_3.566171, whole genome shotgun sequence.
ACCESSION   DAOTIL010000076 DAOTIL010000000
VERSION     DAOTIL010000076.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868050
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminobacteriaceae bacterium (wastewater metagenome)
  ORGANISM  Aminobacteriaceae bacterium
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae.
REFERENCE   1  (bases 1 to 5163)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5163)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:19:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,224
            CDSs (total)                      :: 3,198
            Genes (coding)                    :: 3,184
            CDSs (with protein)               :: 3,184
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5163
                     /organism="Aminobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19073_length_5163_cov_3.566171"
                     /isolate="AMR_MDS_3026"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:3034609"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(144..692)
                     /locus_tag="P5201_02570"
     CDS             complement(144..692)
                     /locus_tag="P5201_02570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008520307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6305 family protein"
                     /protein_id="HRV97447.1"
                     /translation="MKKKMMYLLLLLSIVSFPFSSLADTPKLEGPYLVTTCGQSPGAV
                     MVRMSALQAGVQAEHNNTLSASDLSGKDVKTLIVTTGTSMKGMGAAGTNVDKEIARCS
                     ELIAAAKSAGIVVVGAHVEGMARRTDSSDEASIEAVMKDADVLLVIDDSDSDGFFTEY
                     ARKHEKPLIKIKDALAIGPVLK"
     gene            complement(807..2477)
                     /locus_tag="P5201_02575"
     CDS             complement(807..2477)
                     /locus_tag="P5201_02575"
                     /EC_number="3.4.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008522563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016805 - dipeptidase activity [Evidence
                     IEA];
                     GO_function: GO:0070004 - cysteine-type exopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="C69 family dipeptidase"
                     /protein_id="HRV97448.1"
                     /translation="MKALRFSRVFFACVLAVFVSTSAVACTPMGVGPKASVDGSVMVS
                     HTCDGWYDHRIQIVPGGSHQPGEMVDIFNIMCYDTRPDRPVKKVGEIPQAEKTYTYFH
                     IGYPFMNDQQVMMGEFTWSGRDELGTPEGLFYIENLEALGLARAATAREAIKVMGSLA
                     EKYGYADGGETLVIADTKELWVFEICGPGLWNKESGRPGAHWVAQRVPDDEVFVGANR
                     ARIGVIDFADTENYIFSTDITKLPEQMGWWKQGEPFHYANIFNPEPYGYPYYQSRREW
                     RALSLLAPSQKFDVLDEYEHYPFSIKPDRKLSVKDLMDIYSDHLEGTDYDMTTDAASG
                     PFGNPNRWPTPASVRPENRKGMDWERPIAMFRCSYSFVSQSRDWLPAPIGGVLWFGQD
                     APDTTVYVPIYCGVTELPKPWTTGKRAEFDRESAWWAFNLVNNWANLRWDAMYKEIRA
                     KKAEFEDVFFSLQTEVEEKALALYKKDPQEAVAYLTQYTNANLNKVEKGWWDFAFHLI
                     GKFYDGGMINEEGKMTSPGYPTEYLEKVGFGDLTVRDLERKKARETAK"
     gene            complement(2813..3505)
                     /locus_tag="P5201_02580"
     CDS             complement(2813..3505)
                     /locus_tag="P5201_02580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005660817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HRV97449.1"
                     /translation="MTYINVLVVEDDPMVAEIHQNFINSVDNFRVAGIVNNGLKALEF
                     IERRPVRLVILDIFLPGLDGIATLEKIRERGENIDVIVVSASRDVSTVAKAIQAGAFD
                     YIVKPFVFERIRSSLLSFREMMGHLKGTTQEIDQQTIDTLFQVRNRKHYDSKLPKGLN
                     PHVLERLETMLSGTNVSLSSAEAAEALEISRITARRYLEFLVSTGKAVMEQEYHEVGR
                     PVNKYTLMKQTP"
     gene            complement(3505..>5163)
                     /locus_tag="P5201_02585"
     CDS             complement(3505..>5163)
                     /locus_tag="P5201_02585"
                     /inference="COORDINATES: protein motif:HMM:NF012707.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GHKL domain-containing protein"
                     /protein_id="HRV97450.1"
                     /translation="VPADSELFTFFSVKNMDTADTRLIDDFELMKHRLSGKEFASYCF
                     SLPFFEEPYTLFAAGSEALPLSSLRGKILLVIEDSPEERHIRASAFNVKILQEDTMDS
                     AVHSLLANKGDAALLGAYQGIRHTQNLPQKGRITPSRPFFFSLGKGITVMKGQADLAL
                     AMMERLSAMKQSGEISKITGAWLSQYDPPVLSRQEIWNIVGGGLLALFAALLWNLLLK
                     RKVGIMVKEREKILDFIKDGILAVDKGGRITMLNRSAQNLLGLSSDSIGKEADLCIPG
                     LAVGIVLTDGRPVYNLQQNLNGALVSCNKVPILEDSHSSGVIITLRDMSELQAMAEEI
                     TGVRTYVETLRVQGHEFMNKLQTISGLIQLQRYDRAIEFIASETDSSLSAQAFLAERI
                     KNAAVCGIIMGKAGRCRELGIAFILDPESFCCDHGESINDRSLVIIIGNLLQNAIEAV
                     VETGVTPDARIEFSIFDESGQILIDVFDNAGIMTEEAASRLFEKGFTTKYKAEPSGFG
                     LFNIKTIVDSLAGNISMDFETGRFTEFTVTLPVTAGALRPEVAI"
BASE COUNT         1223 a         1321 c         1328 g         1291 t
ORIGIN      
        1 tctttcctgc agcgaaagct gcaactataa aaattaatac aaagagagaa tgatgaaaca
       61 ttgtcatcgg aatacaatcc tttcagaaaa tgtaaagcaa aaagggcctc aaagagaggc
      121 cctcatgcca acaataaaga tggctacttg aggaccggac cgattgccaa agcatcctta
      181 attttgatta atggtttttc gtgtttccgc gcatattcag tgaaaaatcc gtcgctgtcg
      241 ctgtcgtcga ttacaaggag cacatcggca tccttcatga cggcctcgat agaggcttcg
      301 tcggagctgt cggtccggcg ggccattcct tctacatgcg cgccgacgac cacgattccg
      361 gcagattttg ccgcagcgat gagctcgctg caccgggcga tttccttgtc tacgttcgtt
      421 cccgcagctc ccataccctt catgcttgta ccggtagtga caataagcgt tttcacgtct
      481 tttccggaaa gatccgaagc ggaaagcgtg ttgttgtgtt ccgcctgcac tccagcctga
      541 agcgccgaca tgcgcaccat gacggccccg ggactttgtc cgcaggtggt gaccaaataa
      601 ggaccttcaa gtttcggagt atcggcaagg ctggaaaacg gaaaagaaac aatcgaaagc
      661 aagagaagca aatacatcat cttttttttc atctatgagc acctcctgaa aagtgtattc
      721 tcgcggcgcg tgttttttca tccgggctta tttttatagt aaatcggctg cggggggggg
      781 cagccgattt acctgacgca ctccgactat ttcgcggttt ctctggcttt tttcctttcg
      841 aggtcgcgga ctgtgagatc gccgaatccg actttttcaa ggtactctgt ggggtaaccc
      901 ggcgaggtca tctttccctc ttcattgatc atcccgccat catagaattt accgatgaga
      961 tggaatgcga aatcccacca gcccttttcg actttgttca gattggcatt tgtgtactgg
     1021 gtaagatacg cgactgcctc ctgagggtct ttcttataca gggcaagcgc tttttcctca
     1081 acttccgtct gcagtgagaa gaagacgtct tcaaactccg ccttctttgc tctgatctct
     1141 ttgtacatgg cgtcccagcg cagattggcc cagttgttca caaggttgaa ggcccaccag
     1201 gcgctttcac gatcgaactc cgcacgtttc cccgtggtcc agggtttggg cagctcggtt
     1261 acaccgcagt agatgggaac gtataccgtt gtgtccgggg cgtcctgtcc gaaccagagc
     1321 acaccgccga ttggggcggg gagccagtcg cggctctgtg aaacaaagct gtaagagcag
     1381 cggaacattg cgatcggacg ctcccagtcc attcccttac ggttctcagg gcgaacggaa
     1441 gcgggagttg gccaccggtt gggatttcca aaggggccgg aagccgcgtc cgtggtcata
     1501 tcgtaatcgg taccttcaag atggtcgctg tagatgtcca tgagatcttt tacactcagt
     1561 ttacgatccg gtttgatgga gaatgggtag tgctcatact cgtcgaggac gtcgaatttc
     1621 tgtgaagggg cgagaaggct caaagctctc cattcgcggc gggactgata gtacggatag
     1681 ccgtaaggtt ccggattgaa gatatttgca tagtggaagg gctctccctg tttccaccac
     1741 cccatctgct cggggagctt ggtgatgtcg gtggagaaaa tgtagttttc ggtgtcggcg
     1801 aagtcgatca cgccgatgcg cgcccggttg gctccgacga atacttcatc gtcgggaaca
     1861 cgctgcgcta cccagtgagc gccgggccgg ccggactctt tgttccagag tccggggccg
     1921 cagatctcga atacccaaag ctccttcgta tccgcgatga caagcgtttc tccgccgtcc
     1981 gcataaccgt acttttctgc aagggaaccc atgactttga tggcttcccg cgcggtggct
     2041 gctcgcgcca gaccgagggc ttccagattt tcgatataga aaagtccttc aggagttcca
     2101 agttcatccc gtccgctcca ggtgaactcg cccatcatga cctgctggtc gttcatgaag
     2161 gggtagccga tatggaaata ggtataagtt ttttcagctt ggggaatttc gccgaccttt
     2221 ttcacagggc ggtcggggcg ggtgtcgtag cacatgatat taaaaatatc aaccatttct
     2281 cccggctgat gagagccgcc cgggacaatt tggattcggt ggtcatacca tccgtcgcac
     2341 gtatggctta ccatgaccga tccgtcgacg ctcgcctttg gaccaactcc catgggagta
     2401 cacgccacgg ctgacgtcga tacgaagacc gcaagaacgc atgcgaaaaa gacacgtgaa
     2461 aaacgcagag ctttcatcct tttacctcct aaaaaaatat gtgattggca ttgcctcgca
     2521 cagaaaaatc cgcccccccg cagaagaaaa aaacggagcg tcgtagagac tcttttactc
     2581 tacgtgctcg cagtaaattg cgccagtcgt tttgaaggat gtgcgcattt ttgtttcttt
     2641 ttgttcaaaa ctccctggaa aaaaaggggg aaggggaaac ggaactggcg atggaaactg
     2701 aagaaatctt ttcgccggtt tgtgccaagc cgtgaacaaa aatgtacaaa acgaagagcg
     2761 tgttcaaaaa ttcttatgaa aaagcgtaag aataccgtat acaagactct cgctatgggg
     2821 tttgcttcat gagcgtatat ttattcacag gacgtcccac ctcgtgatac tcctgttcca
     2881 tgacagcttt tcctgttgat accaaaaact ccaggtagcg tctagcggtg atccgtgata
     2941 tttccagcgc ttctgccgct tctgccgaag aaagcgacac attcgtcccg gagagcattg
     3001 tttccagacg ctctagtacg tgcgggttga ggcctttagg gagtttgctg tcgtagtgct
     3061 tcctgtttcg cacctgaaaa agggtatcga tcgtctgctg gtcaatctct tgagttgttc
     3121 ccttgagatg tcccatcatc tcgcggaaag agagaaggga tgaacggatt cgctcgaaaa
     3181 cgaagggttt tacaatatag tcgaatgccc ccgcctgaat tgccttcgca actgtagaaa
     3241 catcccttga tgcggaaacg acgatcacgt caatattctc acctctctcc cgtatttttt
     3301 cgagagtggc tattccgtcc aaaccgggga ggaaaatatc cagaatgacc agtctgacag
     3361 ggcggcgttc tatgaattcc agggccttca gaccgttatt gactattccg gcaacacgga
     3421 aattatcgac ggagttaatg aaattctgat ggatttcagc gaccatcgga tcgtcttcca
     3481 ctacgagaac attgatgtat gtcactatat ggctacctcc ggccggagag cgcccgcggt
     3541 tacaggcaac gtcaccgtaa actcagtaaa gcgcccggtt tcaaaatcca ttgatatgtt
     3601 gccggcgaga gaatcaacta tggtcttgat attgaagagg ccgaaaccgg agggctcggc
     3661 tttgtatttc gttgtgaacc ctttttcgaa aagcctggat gcggcttctt cagtcatgat
     3721 tccggcgtta tcgaagacgt ctatcagtat ctgtcccgat tcgtcgaaga tggaaaactc
     3781 gattcgcgca tcgggagtta ctccggtttc aacgacggct tctatcgcat tttgtaaaag
     3841 atttccaatg atgatcacca gagaccggtc gttgatgctc tctccgtggt cacagcaaaa
     3901 gctctcaggg tcgagtataa aggctattcc cagttcacgg cagcgtcccg ctttgcccat
     3961 gatgattccg cagacggcgg cgttctttat tctctcggcc aggaatgcct gcgccgagag
     4021 acttgagtcc gtttcggatg caatgaattc gatggccctg tcgtagcgtt gcagctgaat
     4081 cagtccggaa attgtctgca acttgttcat gaactcatgg ccctgtaccc tcagggtttc
     4141 tacataggtc cggacgccgg taatctcttc cgccattgcc tgaagctcgg acatatcacg
     4201 aagtgtaatg ataacgccgc tggagtgaga atcctccagt atgggaactt tgttacaaga
     4261 aacgagcgcc ccgttgagat tctgttgaag gttgtagaca ggccgtccat ctgtcagcac
     4321 aatgccgacg gcaagaccgg gaatacagag atcggcttct ttgccgatag agtcggagga
     4381 gagaccgagg agattttgtg cggagcggtt gagcatcgta attcgtccgc ccttatccac
     4441 agcaagaata ccatctttga taaaatcgag aattttctcg cgctctttga ccataatccc
     4501 gaccttccgc ttcagcagta aattccataa gagcgcggca aagagcgcta aaaggcctcc
     4561 tccgactatg ttccatatct cctgtctgct taaaacgggc ggatcgtact gagagagcca
     4621 cgcgcccgtt atcttcgaga tttctcccga ctgtttcatc gccgaaagcc tttccatcat
     4681 tgcaagagcc aggtcagcct gccctttcat aaccgtgatc cctttgccga gcgaaaagaa
     4741 gaacgggcgg gagggggtga tcctcccctt ttggggaagg ttctgcgtgt gccgaatgcc
     4801 ctggtatgct ccaagaagcg ccgcatcccc cttgttcgcg agcagagaat gaaccgcgga
     4861 atccatggtg tcctcttgaa gaatcttgac attgaacgcg cttgccctga tgtgacgttc
     4921 ttcaggagaa tcttcgatga ccagcagtat tttccctctc aagctgctaa gggggagggc
     4981 ttcgctcccg gcggcaaaga gcgtgtacgg ctcttcgaaa aaagggagag aaaaacagta
     5041 agaagcaaac tccttgccgg aaaggcgatg tttcatcagt tcaaagtcat cgataaggcg
     5101 agtgtccgct gtgtccatgt tcttcaccga aaaaaaagta aaaagttccg agtccgcggg
     5161 aac
//
LOCUS       DAOTIL010000077         5144 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminobacteriaceae bacterium isolate AMR_MDS_3026
            NODE_19181_length_5144_cov_3.896443, whole genome shotgun sequence.
ACCESSION   DAOTIL010000077 DAOTIL010000000
VERSION     DAOTIL010000077.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868050
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminobacteriaceae bacterium (wastewater metagenome)
  ORGANISM  Aminobacteriaceae bacterium
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae.
REFERENCE   1  (bases 1 to 5144)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5144)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:19:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,224
            CDSs (total)                      :: 3,198
            Genes (coding)                    :: 3,184
            CDSs (with protein)               :: 3,184
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5144
                     /organism="Aminobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19181_length_5144_cov_3.896443"
                     /isolate="AMR_MDS_3026"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:3034609"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..226
                     /locus_tag="P5201_02590"
     CDS             <1..226
                     /locus_tag="P5201_02590"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="16S rRNA processing protein RimM"
                     /protein_id="HRV97451.1"
                     /translation="EDLKGLSAVRHQDGASLGKLVDVATAGENDIYVIRDDSGNDHYI
                     PAVKQFVAEVNLEKKEIRILLIEGLWESCM"
     gene            217..1479
                     /gene="trmD"
                     /locus_tag="P5201_02595"
     CDS             217..1479
                     /gene="trmD"
                     /locus_tag="P5201_02595"
                     /EC_number="2.1.1.228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013047957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052906 - tRNA
                     (guanine(37)-N(1))-methyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(37)-N1)-methyltransferase TrmD"
                     /protein_id="HRV97452.1"
                     /translation="MHVTIVTAFPEFFEKFLSTSMIGRGIKNGLLHVDVVDLRNFGLG
                     NYRQIDDYAFGGEGGMVLLPEVLERALAAIRESSGTKGYVVYPSPQGDLFSQEIVETL
                     AAQEHIILFCGHYEGLDERFVSRCVDREVSMGDFVLTGGEIPAMAIIDAMARLVPGVV
                     GKETAVVEDSFFRGMLDHPHCTRPAEWNGECVPEELLSGDAAAIENWRRNAAVERTLA
                     RRPDLISRANIRPYLPKGIYVELVHFPVLDRRGEKSTAAVTGLDLSDIARSCKTYGID
                     RFFVTTPLASQRDLVKTMTRHWTEGGGANVNPERAEAFRLLKLFPSVAKSVEWISKRE
                     KEIPLIVGTTARKHPGAFHWIEAKRRMLREKRPILLLFGTGSGLHEEVLAECSMVLSP
                     LSGGGGEYNHLSVRTAAGAVLDRFFGWR"
     gene            1635..1982
                     /gene="rplS"
                     /locus_tag="P5201_02600"
     CDS             1635..1982
                     /gene="rplS"
                     /locus_tag="P5201_02600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013047958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L19"
                     /protein_id="HRV97453.1"
                     /translation="MNVLNLVEKKFLKSDVPEFRSGDTVRVHVKVKEGSRERIQVFEG
                     VVIARKHGGLSETFTVRKISSGVGVERIFPLHCPSISQIEVKRLGKVRRAKLYYLRDR
                     SGKSARIKERREF"
     gene            2034..2120
                     /locus_tag="P5201_02605"
     tRNA            2034..2120
                     /locus_tag="P5201_02605"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2068..2070,aa:Leu,seq:aag)
     gene            complement(2126..2509)
                     /locus_tag="P5201_02610"
     CDS             complement(2126..2509)
                     /locus_tag="P5201_02610"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRV97454.1"
                     /translation="MFRLRRILQHPRSRRFEKLEEVLRRSLLGYGFPQSLPAGQQEAQ
                     EAPPETHRYFCSGLPVSKEETLPPSTSFPNPCSKPPTLDVLPSKEVYQKKSTSSNGKK
                     TFLPFLSKKNHGIQESPLPIQEEGF"
     gene            3031..5103
                     /locus_tag="P5201_02615"
     CDS             3031..5103
                     /locus_tag="P5201_02615"
                     /inference="COORDINATES: protein motif:HMM:NF024824.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HRV97455.1"
                     /translation="MGGLFFASGESFEKILQQGDQAYAQKEYDRALLLYEKAAEKNPL
                     RAEPLFGKARTLEELDRKGEAVHAWNSCLKIAPEDAEVYRRLGDLLFELGSLDNAIRN
                     YQESVRFAPEDSETHFRLGSLLESREDFSQALESYRKALEVDATRDDFAEAVERTEKK
                     VLIADEERRQRTALSEEQIRLGAAFLVTGEFLEAEAHFLRALDLIPEGEDAFFGLAEA
                     RIGKGDIEGARETYLRLLEVTPQSAKARAALLALDPSANDSKEPVLSDDRSMPIAEQQ
                     GKTDAGQLSPDIVSSSITSSSDVSNKTEQSADVAVLRIPGEKNTAEALGEEGSTQAVI
                     PPGSGVTPPPVSADIEIASLPSSADVLSVKPSPVVEITEKNDFPQALVLTPETTPLKE
                     TPLSSDVSSVIGEVVSKEPKERPSKRTETPRAPFNGRVVKSTVSPPLSVPRRSRKSSA
                     PIETGQNTRSIAAFKKNEEKILEASRSYNLSGQYLKAAEKASEALDFEQSPEALSNLG
                     YAHIGLGNYPAAFTAYWRRLLLSPKLRKVSLEPPAFLSLPFKEGRWKEIPVPNPRDLP
                     VVVRSEKMVLLPGGNAVLSGVLPGKEYLFEALRINPTGKDVYLNISLSYLMMQNKGTS
                     SPSFSAAPARSENENALYLALLAHALREKKADEQSEQFLNAAAARASGEVLRLVRSLE
                     KKTSPETK"
BASE COUNT         1243 a         1221 c         1433 g         1247 t
ORIGIN      
        1 agaggatctg aagggccttt cggctgtccg gcatcaagac ggcgcgtctc ttggaaaatt
       61 ggtcgatgtg gcgactgccg gcgaaaacga catttatgta attcgcgacg attccggaaa
      121 tgaccactat atcccggcgg tgaaacagtt tgtcgccgaa gtgaaccttg aaaaaaaaga
      181 gatacgtatt ctgctcatcg aggggctgtg ggagtcgtgc atgtaacgat tgttacagca
      241 ttcccggagt tctttgaaaa atttctgtcc acaagcatga tcggcagagg catcaaaaac
      301 ggtcttctgc atgtcgatgt ggtggatctc aggaacttcg gactcggaaa ctatcgccag
      361 atagacgatt acgctttcgg cggcgaaggc ggaatggtgc ttcttccgga agtgcttgag
      421 agggctcttg ctgcaattcg ggagtcttcg ggtacaaaag ggtatgtggt ctatccatcc
      481 cctcagggcg acctcttttc tcaagaaatt gtggagaccc tggcggcaca ggagcacatc
      541 attcttttct gcggtcatta cgaggggctg gacgaacgct tcgtctcacg atgtgtcgac
      601 agggaggtct ctatgggtga ttttgtcctg accgggggag agatcccggc gatggcgatc
      661 atcgacgcaa tggcccgcct tgttcccggc gttgtgggga aagaaacggc agtagtcgag
      721 gattcatttt tcagaggaat gctggatcac ccacattgta cgcgccctgc ggaatggaat
      781 ggagaatgcg ttccggagga attgctctcc ggcgatgccg cagccattga gaactggcgg
      841 agaaacgccg ccgttgaacg tactctcgca cgccgtcctg atcttatttc ccgtgcgaat
      901 atccgcccct atctcccaaa gggaatttat gtcgagcttg tccactttcc cgttcttgac
      961 agaagaggag agaagagcac cgctgcggtt accgggcttg atctttcgga tattgcccgt
     1021 tcgtgcaaaa cgtacgggat cgaccgtttc ttcgtaacca cgccgttggc gagccagcgt
     1081 gatttggtga agacgatgac tcgtcactgg acggaaggcg gcggggcaaa cgtcaatcct
     1141 gaacgagccg aagcgtttcg tttgctcaaa ctctttccct cggtagcgaa aagcgtcgag
     1201 tggatttcga agagagagaa agagattcct ctcatcgtcg ggacaaccgc cagaaagcat
     1261 cccggcgcat tccactggat tgaggcgaaa agacggatgc tccgtgagaa acggccaatt
     1321 cttttgctct ttggtacagg ttcagggctc catgaggaag ttttagcaga gtgttcgatg
     1381 gtactgtcgc ctctttccgg cggaggggga gagtataatc atctttccgt gagaaccgcc
     1441 gcaggcgccg ttctggatcg gttttttgga tggcgctaga aggaaatcaa caagttccat
     1501 gctcggtgaa agaacgatca cttgaggcct gtcggacttt attttgttca gggagttgtg
     1561 ttttcgccgg atagctgctt gagattgtgt aaagataatc gagttttgtg aaagctcaag
     1621 gagggttcat tgaaatgaac gtgttaaatc ttgtcgagaa aaagttttta aagagcgatg
     1681 ttccggagtt tcgttccggg gacactgttc gggttcatgt gaaggttaag gaaggttcca
     1741 gagagcgcat tcaggtattt gaaggcgttg ttattgcccg taaacacggc ggtctcagcg
     1801 agacattcac cgtccgcaag atctcgagcg gagtcggagt ggaacgtatc ttcccgctgc
     1861 attgcccttc gatcagccag attgaagtga agcgccttgg aaaggtccgc cgcgccaagc
     1921 tgtactatct gcgtgacaga agcggaaagt ccgcccgaat caaagagaga agagaatttt
     1981 agttttcctc tcttgacttt gggaaaaagc ccgcttataa tatccgtcgt tgcgcggggg
     2041 tggcggaaca ggtagacgcg caaggctaag gaccttgtgg ctgcaaggct gtgggagttc
     2101 gagtctcccc ctccgcacca tgaaatcaaa agccctcttc ctgtatggga agagggcttt
     2161 cttgtatgcc gtggtttttc ttcgataaaa acgggagaaa agtttttttc ccattgcttg
     2221 aagtgctttt cttttggtac acttctttgg atggcagtac gtcgagggtg ggtggttttg
     2281 aacatggatt gggaaagctg gttgaggggg gaagagtttc ctcctttgaa acaggaagac
     2341 ccgaacaaaa atagcggtgc gtctctggcg gagcttcttg ggcttcctgt tgtcctgccg
     2401 gaagagactg cggaaaaccg tagcccagca gcgagcgccg gagaacctct tcaagtttct
     2461 caaaccggcg gctccgtgga tgttgaagta tccgacgcag acggaacaat gtcttctctc
     2521 cctcaagcga ctgaaatcca gggtgagtcc tctggtcaac aggacgtgat gtttcctgtt
     2581 gtttcagagg ttgttgaaaa aacagaaacc aggcggcatg ttccagcttc cgtatcagag
     2641 ggagaacctc aagaaacaaa agtctcggaa gcgacggaag aaggaagttc ctctgatacg
     2701 gagaacacag atcaaaccgc gctccccgag acggagagac acgatgatga cttcgtttcc
     2761 tcgagaaaag cggaagagcc gcttgctcct tccattgaac cggatgaaac cggtgtttcc
     2821 gtttctcttc aggaagagag cggcgagagc cgcagcgccg tttctacgtt cacaattccg
     2881 gcccatccgc tctatccttt gattgaagag gattctgccc ctgaaaaaac gatttttgtc
     2941 gaaacgaacc ggactcacgc ccagccgcag cgtgagaaaa acacaaaaaa ccgagtgttg
     3001 ccgatttgtt tgctggcggc agcgcttgta atgggcggac ttttttttgc ttccggagaa
     3061 tcgtttgaaa agatattgca gcaaggcgac caagcttacg cgcaaaaaga gtacgacagg
     3121 gcgttgcttc tttacgaaaa agcggcggag aaaaaccctc ttcgtgcgga acccctcttt
     3181 ggcaaagcgc gaactcttga agagctggat agaaagggag aagccgttca cgcatggaac
     3241 agctgtctga agatcgctcc cgaggatgcg gaggtatata ggcgtctggg cgatttgctt
     3301 ttcgaacttg gttccttgga caacgcgatt aggaactatc aagagagcgt cagatttgca
     3361 ccggaggatt cggaaactca ttttcggctc ggttcccttt tggaatcaag agaggatttt
     3421 tctcaagccc tcgaatcgta tcgcaaggcc ttggaagtag atgctaccag agatgacttc
     3481 gctgaggctg tcgaaaggac cgaaaaaaag gttcttattg cggatgagga gcgccgtcag
     3541 cggaccgctc tgtcggaaga gcaaattcga cttggagccg cttttcttgt aaccggagaa
     3601 tttctggagg cggaagcgca ttttcttcgt gctctcgatc ttattccgga gggagaagat
     3661 gcgtttttcg ggcttgcgga agccaggata gggaagggcg atattgaggg ggcaagagaa
     3721 acctatcttc gacttcttga ggttactcct caatcagcga aagccagagc ggcgcttctt
     3781 gcgcttgatc cttccgcaaa tgacagtaaa gaacctgttc tttccgatga tcgctctatg
     3841 ccgattgctg aacagcaagg gaaaaccgat gccggacagc tttctcccga tattgtttca
     3901 tcaagtatta ccagttcctc agacgtatcg aataaaaccg agcaatctgc cgatgttgct
     3961 gttctgcgga taccgggtga aaaaaacaca gcagaagccc tgggagaaga gggatcaacc
     4021 caagccgtca ttccgccggg aagcggtgtg acgccgcccc ccgtttcagc agatattgag
     4081 atcgcttctc tcccttcctc cgccgacgtt ctttcggtga agccgtctcc tgtcgtcgag
     4141 ataacggaaa agaacgattt tcctcaggcg cttgttctta ctcctgagac tacgcctctg
     4201 aaggagacgc cgctttcaag cgacgtctcc tcggtgatag gggaagtcgt ttccaaagag
     4261 ccgaaggagc gcccttcaaa gagaactgaa acgccgaggg cgccgttcaa cggacgagtg
     4321 gtcaaatcga cggtatcgcc gccattatct gttccgcgac gttcccggaa gagttctgcg
     4381 ccaattgaaa ccgggcaaaa tactcgttct atagcggcgt ttaagaagaa tgaagagaaa
     4441 attcttgagg caagccgttc gtacaatctc tccggacagt atttgaaagc ggcggaaaag
     4501 gcgtccgaag ctcttgactt cgagcaatct ccggaggctc tctcgaatct gggatacgct
     4561 catatcggtc tgggcaatta cccggcggct tttaccgcct actggagacg gttgctgctt
     4621 tctccaaagt tgcggaaggt ttctcttgaa cctcccgcat tcctctccct tccgttcaaa
     4681 gaagggcgct ggaaagagat tcctgttccg aatccaagag atctgcctgt tgttgttcgc
     4741 agcgaaaaaa tggtgcttct gccgggtggg aacgcagtcc tttccggagt tcttccaggc
     4801 aaggagtatc ttttcgaagc gcttcggata aatccaacag ggaaagacgt atatcttaat
     4861 atttcattga gctatcttat gatgcagaac aagggaactt cttctccgtc attttctgcc
     4921 gctccggcca gaagtgagaa tgaaaacgcg ctctatcttg ccttgcttgc ccacgcgctc
     4981 cgtgaaaaaa aagcggatga gcagtcggaa cagtttctta atgctgcagc ggcaagagca
     5041 tcgggagagg tattgcgctt ggttcgttcc ctggagaaga aaacttctcc tgaaacaaag
     5101 taaaatgcag atgtacgctt gaacgctttt ctttcaccag acgc
//
LOCUS       DAOTIL010000078         5136 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminobacteriaceae bacterium isolate AMR_MDS_3026
            NODE_19219_length_5136_cov_3.674670, whole genome shotgun sequence.
ACCESSION   DAOTIL010000078 DAOTIL010000000
VERSION     DAOTIL010000078.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868050
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminobacteriaceae bacterium (wastewater metagenome)
  ORGANISM  Aminobacteriaceae bacterium
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae.
REFERENCE   1  (bases 1 to 5136)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5136)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:19:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,224
            CDSs (total)                      :: 3,198
            Genes (coding)                    :: 3,184
            CDSs (with protein)               :: 3,184
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5136
                     /organism="Aminobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19219_length_5136_cov_3.674670"
                     /isolate="AMR_MDS_3026"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:3034609"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..304)
                     /locus_tag="P5201_02620"
     CDS             complement(<1..304)
                     /locus_tag="P5201_02620"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRV97456.1"
                     /translation="MQCANRIRTILRSLPTLLSVCCLLFFAALPYAGAQERKAPGPVQ
                     VEVKAQDGTLLSGVTVFLGGRYAPSGADGRAVFDGVPEGKQRLAVEHRGRHGVPQAQ"
     gene            complement(427..2004)
                     /locus_tag="P5201_02625"
     CDS             complement(427..2004)
                     /locus_tag="P5201_02625"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRV97457.1"
                     /translation="MRFFSVRRFCVFMIAWAVAGGFSGAASALENTWTYQRIDSLYIE
                     IPDSWSPYSKKGSSGFYTGKPPSGGASVGSLVLLAGREKRNRYEDVLAPLRRSPGGSI
                     EALFHEEEKVVGGKDAVLFTNEISRREKDEAARPFIRMRSVVWKDAAEDGVLLLAVGG
                     DAALFDEHAADIDRMIASVRFDEPPLRQPGRKLEGIGGHAAVGEPVLPPIDLPPIELE
                     SGETAENEETKEGPPAEKTSRERPVFNSDALLEEEIRKARTALSLRDEGELLLAKGDR
                     EGAMDRFRGSLANVPDEDLSAFVETLGRRASEEHKAEEGRASLLRREGMELQRDGKLH
                     EALTKYRESLAVNDDETVREIVLGLEKLLQERASASVAQGEAHEAGGRLEEARAAYAE
                     SLSWVDEEFVRRRIAAVERLIRERQEAASPERVLAATLRNEGKALEEEGRLYEALGKY
                     RESLKSYEEQELLTRADALETELKERARASISEGGALQRAGRYAEALEKFRESRRYYP
                     RSEVDEHIRKLEEFLKK"
     gene            complement(2039..2581)
                     /locus_tag="P5201_02630"
     CDS             complement(2039..2581)
                     /locus_tag="P5201_02630"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRV97458.1"
                     /translation="MTEMRCLRFLPILLILLFLQGAAGPLLASPETAPAASEGAQRLS
                     RLETAALLRGRGVAHEDAGRLENAVRNYEESLLFFPDAEVSQRIDLLKKTLAERTAPL
                     SPPAPLLPEARVSRLEKARALREDGKNAFVRGELHDAARFFEESLSLFEDPELSRFAG
                     AVKKEIELASSLEGLEELFD"
     gene            2821..3606
                     /gene="nudC"
                     /locus_tag="P5201_02635"
     CDS             2821..3606
                     /gene="nudC"
                     /locus_tag="P5201_02635"
                     /EC_number="3.6.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006299764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000210 - NAD+ diphosphatase activity
                     [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(+) diphosphatase"
                     /protein_id="HRV97459.1"
                     /translation="MDLCSTQGAWLLFHNDMVLVKSKEPLTLPSEDEGAAFTDKLLHR
                     GALSPARGAYSWGEYPADEPAPQGFEPVGLRELWALGGDSIFHSAGTAFQMMDWRRNC
                     RYCPRCATLMQPAEEGRAYACPQCAYVSYPTLSPAIIVAVVRDGKLLLAHGTRFPSGR
                     YSVLAGFVEPGESLEETVEREIFEEVGIKVKDIAYFGSQPWPFPHSLMLGFTAHWASG
                     EIVPDGVEIGDADWFTPENMPEIPPSISISRKLIDHWLSGGIA"
     gene            3632..4942
                     /locus_tag="P5201_02640"
     CDS             3632..4942
                     /locus_tag="P5201_02640"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRV97460.1"
                     /translation="MIRALGILVFLAFALALHPNAEAEAKEIEKGLSRGSVLLSWFKD
                     AEMDFQLLRSLGADVAGGGAAGEIFVTAKGITDGNPASWSAAFLALGERLEADGNARL
                     ERGHKVSAMESLLRAASYYRAAEYYGDPSAPETAQTGMKCRDVFMKGMQLAAWKTEGV
                     LIPFGKENDFLPGYFLTPAKGSGKRKTLIAQSGFDGTAEEMYFAVGKAALERGYNVLL
                     FEGPGQTGKRRFHPDSSFVPDCAPALRAVVDFALSRPEVHPAKIALYGASFGGYFALS
                     GAVGEPRLGAVIANSPLIDAQEHFFAGMGPHFVSMFQNQDLTAEEIRAMPEEELPAKY
                     KFSLLSLCVRYGKPSVKETLDVMRDFRLREDQLRSLNIPALGLVSEAEGTVPLAQAKR
                     FAELAQKAALHIFTEESGANIHCQLDNMPLSWAVALDWLDEQFR"
BASE COUNT          952 a         1656 c         1552 g          976 t
ORIGIN      
        1 tctgagcctg tggaacccca tgacgccctc gatgttcaac ggcgagacgc tgttttccct
       61 cggggacgcc gtcgaagacg gcccttccgt ccgcgccgga cggagcgtac ctgcccccga
      121 gaaagaccgt cactccggag agaagcgttc cgtcctgcgc cttgacctcg acctgcacag
      181 ggccgggagc ttttctctcc tgcgctccgg cgtacgggag cgcggcgaaa aagagaagac
      241 agcacaccga cagaagcgtc ggaaggctgc ggagaatagt gcggattctg ttcgcacact
      301 gcatcgcggc tcactctcct tttatagcat ctgcgtgcag taccccggca cgctctttcc
      361 gaacccggct tttcgctccg ggacgacttg aggccggaga ggcgacgtcc gcttctccgg
      421 tacgcgctat tttttcaaga actcctccag cttgcgaata tgctcgtcaa cctcgctgcg
      481 cgggtagtac ctccggcttt cacggaactt ttcgagggcc tccgcatacc tcccggcacg
      541 ttgcagcgct ccgccctcgc tgatactcgc gcgggcacgc tccttcagct ccgtctccaa
      601 cgcgtcggcg cgcgttagca gctcctgttc ctcgtatgat ttcaggcttt cgcggtactt
      661 cccgagcgcc tcgtagaggc gtccctcttc ttccagcgcc ttgccttcgt tccgaagcgt
      721 tgcggcgagg actctctccg gggacgcggc ctcttgccgc tcccggatca ggcgctccac
      781 cgcggcgatt ctgcgccgga cgaactcctc gtcgacccag gaaagactct ccgcgtacgc
      841 ggcgcgcgcc tcctcaagcc gccctccggc ctcgtgcgcc tccccctgcg cgacagaggc
      901 cgaagccctc tcctgcagga gtttttcgag tccgaggacg atctccctca ccgtttcgtc
      961 gtcgttcacg gcgaggctct cacggtactt cgtcagcgcc tcgtgcagtt tcccatcccg
     1021 ctgcaactcc atcccctcgc ggcgcaagag agaagcgcgg ccttcctcgg ccttgtgctc
     1081 ttccgacgcg cgacgcccca aagtctccac gaaggcggac agatcctcgt ccggcacgtt
     1141 cgcgagactt ccgcggaaac ggtccatcgc gccctcccgg tcgcccttcg ccagcaggag
     1201 ctctccttcg tccctgagcg acagcgccgt tcgcgccttc cgaatctcct cttcaagaag
     1261 cgcgtccgaa ttgaagaccg ggcgctctcg cgacgtcttc tccgccggcg gaccttcctt
     1321 cgtttcttcg ttctccgctg tttctccgct ctccagttcg ataggcggca ggtcgatggg
     1381 cggcagaacc ggctccccga cggcggcgtg tcctccgatt ccctcaagct tcctgccggg
     1441 ctgccgcagg ggcggctcgt cgaagcgcac ggaggcgatc atccgatcaa tgtccgcagc
     1501 atgctcgtcg aagagggcag cgtctccccc gaccgcgagg agcagcaccc cgtcctccgc
     1561 cgcgtccttc cagacaacgc tccgcatacg aatgaagggg cgcgccgctt catccttttc
     1621 ccggcggctg atctcgttcg tgaagagaac ggcgtccttc cctccgacaa ctttttcttc
     1681 ctcgtggaag agcgcttcga tgctcccccc gggagatcgc ctcagcggag cgaggacgtc
     1741 ttcgtagcgg ttccttttct ccctcccggc gagcagcacc agggaaccta cggaagcgcc
     1801 tccgcttgga ggtttgccgg tataaaaacc ggaagagcct tttttcgaat agggggacca
     1861 tgaatccgga atttcgatgt acagcgagtc gatacgctgg tacgtccagg tattttcaag
     1921 agcggacgcc gccccggaga aacctcccgc gacagcccag gcgatcatga agacacagaa
     1981 acggcgaaca gaaaagaatc gcatgccaca cacctccata gcgaatagaa tctcccggtc
     2041 aatcgaacag ctcctccagc ccctcaagag aggacgcgag ctcaatctcc ttcttcactg
     2101 cgccggcgaa gcgggagagc tccgggtcct cgaagagcga gaggctttcc tcgaagaaac
     2161 gcgccgcatc gtgaagctcg cccctgacaa acgcattctt gccgtcctcc cgcagagcgc
     2221 gcgctttttc aagacgagaa acccgcgcct cgggaagcag gggcgcgggc ggggagagcg
     2281 gagcggtacg ctcagcgagc gttttcttca aaagatcgat ccgttgcgaa acctcggcgt
     2341 cggggaagaa gagcaggctt tcttcatagt tccggaccgc gttctccagc ctgccggcgt
     2401 cttcatgcgc aacgcctcgc ccccggagaa gagcggccgt ttcaagacgc gaaagacgct
     2461 gcgcgccctc cgatgctgcc ggagccgttt ccggcgaggc gagaagagga cctgccgccc
     2521 cttgcaggaa gaggagaatc agaagaatgg gcaggaagcg caggcatctc atttccgtca
     2581 taacatctct tcctttccat aaaaaagtgg tgtatcaact gccgaatctt atgttttctg
     2641 aattataccc ctcttcgcag gggagtacaa tgggcgtgcg cttcaaaaca gggagcgcct
     2701 gttccgcact ccgcgccttc tccgtgggga aaaaacattg gattcgaaag gcgcgtatga
     2761 tagaattttc gggcggactt ccttcatatt aaggcggccg caaaggagga taacgatcac
     2821 atggatttat gcagtacaca gggcgcctgg ctgctctttc acaacgacat ggttctggta
     2881 aagtcaaaag aacccttgac gcttccatcc gaggacgagg gcgcggcgtt tacggacaag
     2941 ctgctccacc ggggagctct ctcccctgcc cggggcgctt attcatgggg ggagtatccc
     3001 gccgacgaac cggcgccgca gggcttcgag ccggtcggcc tgcgcgagct gtgggccctc
     3061 ggcggagatt cgatctttca ctcggcgggc acggcgtttc agatgatgga ctggcggcgg
     3121 aactgccgct actgcccccg ctgcgccact ctcatgcagc cggccgagga ggggcgggcc
     3181 tacgcctgtc cgcagtgcgc gtacgtctcc taccctacgc tcagcccggc gattatcgtg
     3241 gcggtcgtgc gcgacggcaa gcttcttctg gcgcacggaa cccgctttcc ttcggggcgc
     3301 tacagcgtgc tcgcggggtt cgtcgagccg ggcgagagcc tggaagagac tgtcgaacgg
     3361 gagatcttcg aagaagtcgg catcaaggtg aaggacatcg cctacttcgg cagtcagccg
     3421 tggccgtttc cccattcgct gatgctcggc ttcaccgcgc actgggcaag cggggagatc
     3481 gttccggacg gagtcgagat aggggacgcc gactggttca cacccgagaa tatgccggag
     3541 atccctccat ccatcagcat ttcgcgaaaa ctgatcgacc actggctctc cggaggtatt
     3601 gcgtaacaca gtcagggagg tggaacaacg gatgatacga gcgctcggaa tactggtatt
     3661 ccttgccttc gccctcgcgt tgcatccgaa cgcggaagcg gaggcgaagg agatcgagaa
     3721 gggactgtcg cgcggcagcg tcctgctctc atggttcaag gacgcggaga tggactttca
     3781 gcttctccgc agccttggcg cggacgttgc ggggggcgga gcggcagggg aaatcttcgt
     3841 gaccgcgaag gggataacgg acggaaatcc ggcctcgtgg tcggcggcct ttctcgcgct
     3901 gggagagcgg ctggaagcgg acggaaacgc ccggctggag cgggggcaca aggtcagcgc
     3961 gatggagagc ctgctgcgcg ccgcgtcgta ctacagggcg gcggagtact atggagatcc
     4021 gtcggctccc gaaaccgcgc agacgggaat gaaatgccgc gacgtcttca tgaaggggat
     4081 gcagcttgcg gcgtggaaga cggagggcgt gctcattccg ttcggcaagg aaaacgattt
     4141 tctgcccggc tacttcctca ctccggcgaa gggaagcggc aagcggaaga cgttgatcgc
     4201 gcagagcggc ttcgacggca cggcggagga gatgtacttc gccgtcggaa aggcggcgct
     4261 tgaacggggg tacaacgtgc tcctcttcga gggaccggga cagacgggga agcggcgttt
     4321 ccaccccgac tcgtccttcg tccccgattg cgcccccgca ttgcgggccg tcgtggactt
     4381 cgcgctctcc cgccccgaag tacatccggc aaagattgcg ctctacgggg ccagtttcgg
     4441 cggctacttc gccctctccg gcgcggtcgg cgaaccccgg ctgggcgcgg tgatcgcgaa
     4501 ctcgccgctc atcgacgcgc aggaacattt cttcgccggc atgggaccgc attttgtttc
     4561 catgtttcag aatcaggatc tcacggcgga ggaaatacgc gccatgccgg aggaggagct
     4621 gcccgcgaaa tacaaattct ccctgctgag cctctgcgtg cgctacggca aaccttcggt
     4681 gaaggagacg ctcgacgtca tgcgggactt ccggctgcgc gaagaccagc tccgctcgct
     4741 gaacataccc gcgctgggac tcgtcagcga agcggaggga acagtccccc ttgcgcaggc
     4801 gaagcgattc gcggagctgg ctcaaaaggc cgcgctccac atcttcacgg aggagagcgg
     4861 cgcgaacatc cactgccagc tcgacaacat gcccctctcg tgggcggttg cgctcgactg
     4921 gctggacgag cagttccggt aggcacaaca acaaaggaac ggggcgagga acgccgaaaa
     4981 gcgccccgcc ccgttctttt gttttgaatt tatccccttg cgactacagt acagggagga
     5041 aaaactttct aatgcaccgc gctgttcgcg atgtcctcgg ccgtgacctt gaagtactcc
     5101 ttccgcgcgt tttccggcgt cgaaagttcg cccacg
//
LOCUS       DAOTIL010000079         5105 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminobacteriaceae bacterium isolate AMR_MDS_3026
            NODE_19382_length_5105_cov_3.882970, whole genome shotgun sequence.
ACCESSION   DAOTIL010000079 DAOTIL010000000
VERSION     DAOTIL010000079.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868050
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminobacteriaceae bacterium (wastewater metagenome)
  ORGANISM  Aminobacteriaceae bacterium
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae.
REFERENCE   1  (bases 1 to 5105)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5105)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:19:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,224
            CDSs (total)                      :: 3,198
            Genes (coding)                    :: 3,184
            CDSs (with protein)               :: 3,184
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5105
                     /organism="Aminobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19382_length_5105_cov_3.882970"
                     /isolate="AMR_MDS_3026"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:3034609"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..271)
                     /locus_tag="P5201_02645"
     CDS             complement(<1..271)
                     /locus_tag="P5201_02645"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRV97461.1"
                     /translation="MENNGRLKRDRLASFAFFALGIGCFLLAGFLVYLFSGKFVNTPS
                     GMRAGPALSLSQVSAVTESAPAVLKEPPAAQKAWVLYVTGEVLSPG"
     gene            complement(291..1646)
                     /gene="miaB"
                     /locus_tag="P5201_02650"
     CDS             complement(291..1646)
                     /gene="miaB"
                     /locus_tag="P5201_02650"
                     /EC_number="2.8.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013048761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016782 - transferase activity,
                     transferring sulphur-containing groups [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (N6-isopentenyl
                     adenosine(37)-C2)-methylthiotransferase MiaB"
                     /protein_id="HRV97462.1"
                     /translation="MHRFALKIYGCQMNVYDGDKIRTALVARGWRESPEDEADLVILN
                     GCSIRDKAEQKVWSDLGRFAVRWKNERKPIVAVTGCVAQSVGRRMAARFPWVRFVSGP
                     RHIGAVPDALEQFFTERDAGRTIGNEETVFLLDDDPRAFLDLPGAPTERVNRWKAFVT
                     IAHGCDNFCSYCIVPYVRGRFVSRPAEDILEEMRTLVADGVLEITLLGQNVNSYGSDY
                     SDGYRFSSLLRDAAASTGVRLLRFVTSHPRDFTPDIVEAMAEHTDILCPSINLPIQSG
                     SDRILKMMNRGYTLEQYRAAVSLIRNRLPGAGLTTDLIVGHPGETDDDFQCSVNALRE
                     FRFDLVHSAAYSPREGTSAAAREDQIPREVKMARLNEINRIQSLIAREINESLVGSRF
                     SVLLDDVAPRGEGILQGRTPSDKVVLVEGGEELLGRFVSVEITCGENWCLNGRVLHVE
                     N"
     gene            1867..2331
                     /locus_tag="P5201_02655"
     CDS             1867..2331
                     /locus_tag="P5201_02655"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRV97463.1"
                     /translation="MRRNSCCSVLILFVLLCGGPALAAVSLPDIPGYTAGALRSTPLS
                     APSGDFGVWLSRTYRSEGRRLLVATLMSGPGAGPLVTGEEGTKTDDRPLGFGATYEVF
                     TLDGKPAVYETIPSVGVSLAVAVDANSTLTLESSSLGREALEEAALCMMRAM"
     gene            complement(2406..2567)
                     /locus_tag="P5201_02660"
     CDS             complement(2406..2567)
                     /locus_tag="P5201_02660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006583293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rubredoxin"
                     /protein_id="HRV97464.1"
                     /translation="MKKYVCTVCGYVYDPAEGDPDSGIAPGTAFEDIPEDWVCPVCAV
                     GKDMFEPEE"
     gene            complement(2696..3802)
                     /locus_tag="P5201_02665"
     CDS             complement(2696..3802)
                     /locus_tag="P5201_02665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013048765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase P family protein"
                     /protein_id="HRV97465.1"
                     /translation="MSFDHISRRLQRLRSVVAEEGMDVFVLLVTEGYNSESAAYISGF
                     RGSSCAVVISADRALLVTDGRYALQAKAQSPFSVVLQEQNTLQEKTAELLRGGGWTVA
                     GFEGERLSVSLFRAFEKSVPHWKDCSVLIPSLRRRKDELEIAAIAKAADIASASYEEV
                     LGLVREGMSETELNALLEYAVRKRGAEAGWKGSGFIVASGERSALPHGKATDRTFRKG
                     DIVTVDFGATVDGYMSDLTRNFSLGPLSEGAREIESVLLEAADKAAEALKPGAEGKFV
                     DSVARGIIASKGWGKHFSHGLGHGLGLEIHEAPRLSPVSKDILREGDIVTIEPGIYIE
                     GWGGMRIEDDYLVTSSGSVCISGGKGRKTAVVSV"
     gene            complement(3799..4782)
                     /locus_tag="P5201_02670"
     CDS             complement(3799..4782)
                     /locus_tag="P5201_02670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006301221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AIR synthase-related protein"
                     /protein_id="HRV97466.1"
                     /translation="MEQQLVGKLPPSLLEGSVFRFCGAPRPEVIIGPGIGEDAALLQL
                     PEGKFLAVASDPIVGAREGAGKFLVHINANDIACKGGDPSFFVATLLVPSADGVPMVQ
                     ALMREIHEACLSIGAAVIGGHTELTSRYDQPVLVGTMMGPTAFVHRATDIRPGDVLIL
                     TKHAGLEGMAILANDRPDLLLPAIGPEGVEEARGWLEQISVLEEAKLLRGMARFLHDP
                     TEGGVGGGIAEIVRLSGLGVELDYGSVPFSPLTKKAVQTLDFDPLHLISSGVLLAVVP
                     QGGEADALAALREKGISGVAAGHFVEGAGNCPDDVREELWGLLERPKERRE"
     gene            complement(4739..>5105)
                     /locus_tag="P5201_02675"
     CDS             complement(4739..>5105)
                     /locus_tag="P5201_02675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014806304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="epoxyqueuosine reductase QueH"
                     /protein_id="HRV97467.1"
                     /translation="SHLADEPEGGARCALCFRLQLEAAARRASAMKIPLLCTTLTISP
                     HKNAELINSIGAECASQYGVAWLARVFRKKNGFVRSVALSKEYGLYRQSYCGCLYSMT
                     RGGNNHGTAARGEASPVAP"
BASE COUNT         1142 a         1564 c         1345 g         1054 t
ORIGIN      
        1 ctccgggcga cagcacttcg ccggtaacat agaggaccca cgccttttga gcggcggggg
       61 gttccttgag aacggcaggc gccgattctg ttaccgctga aacctgagaa agcgagagcg
      121 caggaccggc tctcatcccc gaaggcgtat tcacgaattt gccggagaag agatacacca
      181 ggaatcccgc gagaagaaaa cagccgatac caagggcaaa aaaagcgaaa ctcgcaaggc
      241 gatcccgctt cagtctcccg ttattctcca tggtttcact gaatcctcct ttagttttcg
      301 acgtgcagta cgcgcccgtt caagcaccag ttctctccgc atgttatctc cactgagaca
      361 aaacgcccga ggagttcctc cccgccctcc acaaggacga ccttgtccga cggcgttctg
      421 ccctgaagga ttccttcccc cctcggagcg acgtcgtcaa gcagcacgct gaagcggctg
      481 ccgacaagcg attcgttgat ctcccgcgct atgagcgact gaatcctgtt gatttcgttg
      541 agacgcgcca tcttgacttc acggggtatc tggtcctctc tggcggctgc cgaggtaccc
      601 tcccttgggg aataggcggc cgagtggacc aggtcaaaac ggaattccct cagagcattc
      661 accgagcatt ggaagtcatc atccgtttcc cccggatgac cgacgatgag atcggtagtc
      721 aaccccgcgc cgggaaggcg gtttcgtatt aacgatactg ccgcacggta ctgctccagg
      781 gtgtaccccc ggttcatcat ttttaaaatc cggtcgctcc ccgactgaat agggagatta
      841 atcgaaggac agagaatatc cgtatgttcc gccatggctt cgacgatatc cggggtgaaa
      901 tcccgcggat gcgacgtgac gaagcggaga agacgcacgc ctgtggaggc tgctgcatct
      961 cgaagcaggg atgagaatct gtaaccgtcg gagtaatcgc tcccgtagct gttgacgttt
     1021 tgtccaagga gggtgatttc aaggacgccg tcggctacaa gcgtccgcat ctcctccaga
     1081 atatcctcag caggacggga gacaaatcgc ccccggacat acgggacaat acagtatgaa
     1141 caaaaattgt cgcagccgtg agcgatggtg acgaatgctt tccaccggtt cacacgttcc
     1201 gtaggagcgc ccggaagatc aagaaaagct cttggatcgt cgtccagaag aaaaaccgtc
     1261 tcctcgtttc cgatggtcct tcccgcgtcc ctttcggtga aaaactgttc taaagcatcg
     1321 ggaaccgcgc cgatgtgccg gggacccgac acgaaacgaa cccacgggaa acgggcggcc
     1381 atcctgcgcc caacgctctg cgcaacacag cccgtcactg cgacgatcgg ctttcgttca
     1441 ttcttccaac ggacggcgaa acggccgagg tcgctccata ctttctgttc cgccttgtcc
     1501 cgaatgctgc acccgttaag gatcacgaga tcagcttcgt cctcggggct ttctctccac
     1561 ccgcgagcaa caagagcggt tcgtatcttg tcgccgtcgt agacgttcat ctgacatcca
     1621 taaatcttca gtgcaaagcg gtgcaaagaa aaaactcctc ccgatgcgaa aatagaggta
     1681 aaaacaacag aaatagtatt atataccctt caaagggaat tttggtacgt caaaaactca
     1741 atccgtacgt tcacgcatcc tctttttcac caaggaagaa gaacggaaga aacgcgcaag
     1801 gaaaaaccgt ttcgatagag tacaatggta cccgggtttt gaacagagaa tcaaggaggt
     1861 tgcgatatgc gaagaaacag ttgctgtagt gtgctgatac tgtttgtact cctctgcgga
     1921 gggccggccc tcgccgccgt ttcacttccg gatatcccgg ggtatacggc cggggcgttg
     1981 cggagtacgc ctttatctgc gccgtcggga gatttcggag tgtggctctc ccgaacctac
     2041 cggtctgagg ggcggaggct ccttgtagcg acgctgatgt ctggtcccgg agcgggtcct
     2101 cttgtgaccg gagaagaggg aacaaaaacg gacgaccgcc ctctgggctt cggagcgacg
     2161 tacgaagttt ttacccttga cgggaaaccg gcagtatatg aaacaatccc ttcagtcggc
     2221 gtctctcttg ccgttgctgt ggacgcaaac agcacgctca cgctggagtc ttcctccctg
     2281 ggcagggaag cgttggaaga agctgcgctg tgtatgatgc gcgccatgta atcagctgtt
     2341 tttcacaata aagaagggct cccgtaaagg agcccttttt ctgcttcgtg atcacacacg
     2401 caagactact cttcaggctc gaacatatcc ttccccacag cgcagacagg gcagacccaa
     2461 tcttcgggga tatcttcaaa ggccgtcccc ggagcaattc cggaatcggg atcgccctcg
     2521 gcgggatcgt aaacgtaacc gcatacggtg cacacgtact tcttcatgat atttccccct
     2581 tcatcaattc ggaatcagat acaacttcat tatatcgggc gcagagaaaa aggcaactgc
     2641 ctgttgccga aatccctgtt ccttctgttg acaatcccct gacgctccag tggaatcaga
     2701 cggatacaac cgcagtcttc ctgcctttcc cgccggagat acagaccgaa ccgctcgagg
     2761 tcacgagata gtcgtcctcg atgcgcatac ctccccaccc ttcgatgtag ataccgggct
     2821 cgattgtcac gatatcccct tcacggagaa tatccttcga aacgggtgaa agacgcggcg
     2881 cttcatggat ctccaacccg agtccgtgcc ccagtccgtg agagaagtgt ttgccccacc
     2941 cttttgaagc gattattccg cgggctacgg agtcgacgaa cttcccttcc gctccgggct
     3001 tcaaggcctc cgccgcttta tccgccgcct ccagaagcac ggattcaatc tcccgcgctc
     3061 cctctgacag cgggcccagc gaaaagttcc gggtaaggtc ggacatgtat ccgtccaccg
     3121 tagcgccgaa atcgacggtg actatgtctc cttttcgaaa cgtcctgtcc gtcgcctttc
     3181 catgagggag cgcgcttcgc tctccggaag cgacgataaa gccgctcccc ttccaccccg
     3241 cctccgcgcc tctttttctg acggcgtatt cgagaagcgc attcagctcg gtctcggaca
     3301 ttccctcgcg aacgaggccg aggacttctt cataggaagc agaggctatg tccgccgcct
     3361 tggcgattgc ggcaatctcc aattcatcct ttcgtcttcg gagcgaagga atgagaacag
     3421 agcagtcttt ccagtgcggg acggattttt caaacgcccg aaagagagag acggagagcc
     3481 gctccccttc aaaaccggcg accgtccacc ctcccccgcg cagcagttcc gctgttttct
     3541 cctgaagagt attctgttcc tgcaaaacga ctgaaaacgg ggactgcgcc ttcgcctgaa
     3601 gagcatacct tccgtcagtc actaaaagcg ccctgtccgc ggatatcacg accgcgcagc
     3661 tgctcccccg aaaaccggag atgtaggcgg ccgattcgct gttatagcct tcggtaacca
     3721 ggagaacgaa gacatccata ccctcctcgg ccacgacgct tcgcagcctc tgaagacggc
     3781 gtgaaatatg atcgaaactc attccctccg ctccttcggc ctctccaaca acccccagag
     3841 ctcttcgcgg acatcgtccg gacagttgcc cgcgccttcg acaaaatgtc cggccgcgac
     3901 gccggaaata cctttttcac gaagagccgc gagcgcgtcg gcttcccccc cctgcggaac
     3961 aaccgccaga aggactccgg aagagataag atgcagcgga tcgaaatcga gcgtctgtac
     4021 agccttcttc gtaagaggag agaacggcac gctcccgtaa tcgagttcaa ctccaagacc
     4081 gctcaagcga acgatctccg ctattccgcc tccgactcct ccctctgtcg ggtcgtggag
     4141 aaaacgcgcc atgccccgga gaagcttcgc ctcttcgagg accgagatct gttccagcca
     4201 cccgcgcgcc tcctccaccc cttcgggacc tatggcaggg agcagaaggt ccggacggtc
     4261 gttggccaga atagccatcc cctccagccc cgcgtgcttg gtgaggatga gaacatctcc
     4321 gggacgtata tcggttgccc tgtgtacaaa agccgtcggg cccatcatcg tgccgacgag
     4381 gaccggctga tcataccgtg aagtcagctc ggtgtgccct cctattacgg ccgcgccgat
     4441 ggagagacag gcttcgtgaa tctcccgcat aagcgcctgt accatgggaa cgccgtccgc
     4501 ggaaggaaca agaagcgtcg ccacgaaaaa agaggggtcc ccgcccttgc aggcgatatc
     4561 gttcgcgttg atatgcacca gaaatttccc cgctccctcg cgggcgccca cgatgggatc
     4621 ggatgctacg gcgagaaatt tcccttccgg caactgaaga agcgccgcgt cctccccgat
     4681 tccggggccg atgatgactt cagggcgcgg cgcgccgcaa aaacgaaaaa cgctcccttc
     4741 aaggagcgac gggggaagct tccccacgag ctgctgttcc atgattgttt ccacctctcg
     4801 tcatgctgta caggcagccg cagtacgact gcctgtacag gccatattcc tttgacagcg
     4861 ccacggagcg gacaaaaccg tttttctttc tgaagacacg ggcgagccac gcaactccgt
     4921 actgcgaagc acactccgct cctatggagt tgatcagttc ggcgttcttg tgcggactta
     4981 tcgtcagggt tgtgcacaac agaggaatct tcattgctga agctctcctg gctgccgctt
     5041 ccagttgaag gcggaaacaa agggcgcagc gcgccccccc ttccggttcg tccgccaggt
     5101 gcgat
//
LOCUS       DAOTIL010000080         5099 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminobacteriaceae bacterium isolate AMR_MDS_3026
            NODE_19415_length_5099_cov_3.880254, whole genome shotgun sequence.
ACCESSION   DAOTIL010000080 DAOTIL010000000
VERSION     DAOTIL010000080.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868050
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminobacteriaceae bacterium (wastewater metagenome)
  ORGANISM  Aminobacteriaceae bacterium
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae.
REFERENCE   1  (bases 1 to 5099)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5099)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:19:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,224
            CDSs (total)                      :: 3,198
            Genes (coding)                    :: 3,184
            CDSs (with protein)               :: 3,184
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5099
                     /organism="Aminobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19415_length_5099_cov_3.880254"
                     /isolate="AMR_MDS_3026"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:3034609"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(126..611)
                     /locus_tag="P5201_02680"
     CDS             complement(126..611)
                     /locus_tag="P5201_02680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005661222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheX"
                     /protein_id="HRV97468.1"
                     /translation="MGTEKLATLVNTFGSSLMTVSKEMGVELTLAKSEIAQGVNVQGS
                     CLAALVGVVGGGVQGTAVIMLDEGGFRATVSAMSGGMITPNLEDPVSMSVIGELANMV
                     SGRALIQAALEGTEVTPPQLISGAGIKTIPNQSPGVRSFTLPFKLSAGGTLFLVLSFS
                     V"
     gene            complement(632..1087)
                     /locus_tag="P5201_02685"
     CDS             complement(632..1087)
                     /locus_tag="P5201_02685"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease"
                     /protein_id="HRV97469.1"
                     /translation="MFLGIDPGRDKVGWALTDAEGELLLSGIFRAGDAALFFSAFESG
                     ALERLAPFILEKTSGEIADFASAELLMGDGTGKEYLLSLAQSASLGVKLVSERETTLL
                     ARKLYWKLHPPRGVWRLLSAGLRVPPRDIDDLAAWAIVLRYCGVQSKEK"
     gene            complement(1090..1968)
                     /locus_tag="P5201_02690"
     CDS             complement(1090..1968)
                     /locus_tag="P5201_02690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005661218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HRV97470.1"
                     /translation="MKDFTEVKWKALSGVLRLLKRMPLTWSVRFGASLGWLLWALDKK
                     KVDEAERRCVRALQVGPTIARGIVRRSYMNLGRSVAEFASAPGSGRSMRSIVHVHGEE
                     HLREALDSGNGVIFFSGHIGNWEFGAAVLAEMGYPMNAIGADQRDERVTDLIIGLRAV
                     SGVTTVSKGFDLKAAIRCLKRGEALAILIDQDVRERGVVVPFLGIPASTPYGPAKIAG
                     KIGSVILPSFMLRRGTSEEHDLHILPSPWKNGRPEPDESMEEAMARCNDLISEYIRRA
                     PDQWMWLYPRWASTGE"
     gene            2030..2833
                     /locus_tag="P5201_02695"
     CDS             2030..2833
                     /locus_tag="P5201_02695"
                     /inference="COORDINATES: protein motif:HMM:NF012791.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0032559 - adenyl ribonucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HRV97471.1"
                     /translation="MLTRILFFADVSSFSERALAWAARQVAGDRSDFLILHVVDRAAG
                     LDAPELVREAEGYLEEMCARTLPPESYYKTLVLLGDLLESLPDTIRSEKCTFAIFPLP
                     GGEDGMPLVRATPAPQILLREHEGFFPEEDTFGSLAVALDLEPSRTSLMLDNLREFLA
                     QARKNPKITLLHALAPQSAEDAPEVLNAGRQALEEVRDEISSWGYETAAEIVSGDPSK
                     ELPARIRELAPSLLVIGLPVAGELGELVFGSTAEMLFAGTTCPLLVFPL"
     gene            complement(2857..3255)
                     /locus_tag="P5201_02700"
     CDS             complement(2857..3255)
                     /locus_tag="P5201_02700"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012792.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HRV97472.1"
                     /translation="MEERSSVAPWPRTLIRRDLAGDTPMLYLGAWGKDILLGFAALGP
                     CEKGTELTNIAVLPEYRRKGIGSQLVLGVSEVALSLGYSRIYLHVRVSHEESRTFYGG
                     FDFSPLYVVPGYYLDGEDALFMEALLPLSV"
     gene            complement(3331..4617)
                     /locus_tag="P5201_02705"
     CDS             complement(3331..4617)
                     /locus_tag="P5201_02705"
                     /EC_number="6.3.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005658819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004019 - adenylosuccinate synthase
                     activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate synthase"
                     /protein_id="HRV97473.1"
                     /translation="MKGRVEVIIGAQWGDEGKGRVVDALGAKVDIFARYQGGANAGHT
                     VIVEGQKYVFHLLPSGMLYPCKICIIGNGVVIDPEQLLKELKDLQDQGKDRARLVISG
                     AAHVVMPYHKVLDKAQEELRGKGRKIGTTGRGIGPAYVDKYSRCGIRVEDLLDGDVLR
                     EKLEYNLDEKNTYLSKIYGEEPLPFHEMYSIALSWGKSLAPYVGDASLIIDEALREGK
                     GVLFEGAQGTLLDIDHGTYPYVTSSSPVAAGGCVGLGVGPSMVGRVIGVVKAYCTRVG
                     EGPFPTEDCGDEGNKLREKGAEFGATTGRPRRCGWLDLVALRYAVRVNGISSIALTKL
                     DVLTGQERIPVCTSYMVEGQDEPHFPCALQRLEKASPHYEYLEGWHEDIASCTSYEQL
                     PPQARKYVEHIESATGVPVNLIGVGPGRDQTILRNF"
     gene            complement(4690..>5099)
                     /locus_tag="P5201_02710"
     CDS             complement(4690..>5099)
                     /locus_tag="P5201_02710"
                     /inference="COORDINATES: protein motif:HMM:NF016623.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="divergent polysaccharide deacetylase family
                     protein"
                     /protein_id="HRV97474.1"
                     /translation="AVEVLPDAVGVNNHRGSRATADEELMDSFMEILSGFSLFFLDSR
                     TSASSAAYAKAVEKGVPALYSSVFLDHQADETFMETQFLRALALAKRRGWVVVIAHTR
                     IATLQYLKKLEGALPDGYIFVSLPDLFHLLRIR"
BASE COUNT         1098 a         1627 c         1334 g         1040 t
ORIGIN      
        1 cttccaccga aggagagaaa ctgtaatcgc ctgtgggcga actactgaag ctcaccaaca
       61 aacacgaaga ggacggtatc tccccgtcct cttttttctt caaagaaaaa cccgcacgtt
      121 tggaactata cactgaacga gagtaccagg aaaagcgtcc cccccgccga gagcttgaag
      181 gggagcgtga aactgcgaac tccgggagac tgattcggaa tagtcttgat tcccgcgccg
      241 gaaataagct gcggaggcgt cacctcggtc ccctcaagcg cagcctgaat cagggcgcgt
      301 ccgctcacca tgttcgcaag ctcgccgatg acgctcatcg agacaggatc ttcaaggttc
      361 ggcgtaatca tacctccgga catcgcggat accgtcgccc ggaagccccc ctcgtccaac
      421 atgatcacgg cggttccctg gacgccacct ccgacgacgc caacaagcgc ggcaaggcag
      481 ctcccctgaa cattcactcc ctgggcgatc tcggacttcg cgagagtcag ctcaacgccc
      541 atctccttgc tgaccgtcat cagagaagac ccgaatgtat taacaagcgt cgccagcttc
      601 tccgttccca tttgctttca cccttccctt tctacttctc cttcgactgc acaccgcagt
      661 agcgaagaac gatagcccat gcggcgagat cgtctatatc cctcggagga acgcggagac
      721 cggcggaaag cagtctccac acaccgcgcg gaggatggag tttccaatag agtttcctcg
      781 ccaggagcgt tgtctctctt tcagacacta actttactcc aagagaggcg gattgcgcaa
      841 gagagagaag gtattctttt ccagttccgt ctcccataag caactcggcg gacgcaaaat
      901 ccgcaatctc accggaggtt ttttcaagaa tgaacggcgc aagccgctcc aatgccccgg
      961 attcgaacgc gctgaaaaaa agagccgcgt cccccgcccg gaaaatcccc gacaggagaa
     1021 gctctccctc agcatccgta agggcccatc cgactttgtc cctgccgggg tcgatcccga
     1081 gaaacatcgc tactctcccg tcgacgccca tcggggatag agccacatcc actggtcggg
     1141 agcacggcgg atatactcgc tgatcagatc gttgcagcgg gccatggcct cctccatcga
     1201 ttcgtccggt tccggcctcc cgtttttcca cggggagggc agaatatgaa gatcgtgctc
     1261 ctccgaggtt cccctccgca gcataaaact cggcaggatg acggagccga ttttcccggc
     1321 gatcttcgcc ggaccgtagg gtgtgctcgc gggtatcccc agaaacggga cgacgacgcc
     1381 tctctcacga acatcctgat cgatcaggat ggcaagggct tcgcccctct tgaggcatcg
     1441 tatggcagcc ttgaggtcga atcctttgct cacggttgtg accccgctaa ccgcgcgaag
     1501 tccgatgata aggtcggtca ctctctcgtc gcgctggtcc gcgccgatgg cgttcatcgg
     1561 gtatcccatc tccgccagca ccgccgcgcc gaactcccag tttccgatgt gccccgagaa
     1621 aaaaatgacg ccgttaccgg aatccaatgc ctcgcgaagg tgttcctcac cgtgaacatg
     1681 cacgatcgag cgcatacttc taccgcttcc cggcgccgag gcgaactcag caacggaacg
     1741 ccccaggttc atgtaggatc tgcggactat tcccctggcg atcgtcggcc ccacctgaag
     1801 cgcgcgaacg caccgccgtt ccgcctcatc cacctttttc ttatcgagcg cccagagcag
     1861 ccacccgagc gacgctccaa agcgcaccga ccaagtcaac ggcattctct tcaagagccg
     1921 aagaacaccc gacaaggcct tccattttac ctcagtaaaa tccttcaccg ccgcaacccc
     1981 tttgcgctat acttaaagca cacatacaat accatgggag ggatgaaaca tgctcactcg
     2041 aattctcttt ttcgcagacg tctcctcatt ttccgaaaga gccctggcat gggcggccag
     2101 gcaagtcgcg ggagatcgtt cggatttcct gattcttcat gtagtcgacc gggccgcggg
     2161 gctcgacgct cccgagctgg tcagggaggc cgaggggtac ctggaagaga tgtgcgcgcg
     2221 aacgctcccc cctgaatcct actacaagac cctggtcctt ctgggagatc tgcttgagtc
     2281 tcttcccgat accatccgct ccgaaaagtg cacgttcgca atcttccctc ttcccggcgg
     2341 ggaggacggg atgccgctcg tccgggcaac gccggcgcct cagattctcc tgcgggaaca
     2401 tgaggggttt tttcctgagg aggatacctt tggctccctc gccgtcgctc ttgaccttga
     2461 accttcccgg acaagtctga tgctggacaa tcttcgggaa ttccttgcgc aggcacgaaa
     2521 gaacccaaag atcaccctgc tccatgcact cgctccccag tccgcggagg acgctcccga
     2581 agttctcaat gccggccggc aagctcttga agaggttcgt gacgaaatct cctcctgggg
     2641 gtatgaaacc gcagcggaga tcgtcagcgg cgatccgtcg aaggagcttc cggcgagaat
     2701 acgggaactc gcgccgtcgc tccttgtcat cgggctccct gttgcgggcg agctgggaga
     2761 gctcgtcttc gggagcaccg cggagatgct tttcgcggga acgacctgtc ctctgcttgt
     2821 gttccctctg tagagcgtgc atatcttccg ccgcgcttaa acggaaagcg gcagcagcgc
     2881 ttccatgaag agcgcgtctt caccgtcgag gtagtacccg ggaacgacat agagcggaga
     2941 aaagtcgaat cccccgtaaa aagtacggct ttcttcgtgg gaaactcgaa catgcaggta
     3001 tattctggag tatccgaggg agagcgcaac ctcggatact ccgagtacaa gctgggaacc
     3061 tattcccttt ctcctgtatt cagggagcac cgcgatgttc gtgagctcag tccctttctc
     3121 acaaggcccg agagccgcga aacccagcaa aatatcctta ccccacgccc cgaggtacag
     3181 catcggcgtg tctccggcca aatcgcgccg gatgagagtt cgaggccaag gagcaaccga
     3241 cgagcgctcc tccacaaaac agacgtccgg cgcatccttg agagaacagt atcgaatatc
     3301 agccaggaaa attggaatcc cttctcttcg tcagaaattc ctgagaatcg tctgatccct
     3361 tccggggccg acgccgatga gattcaccgg aactccggtc gcggactcga tatgctccac
     3421 gtacttccgg gcctgcggag gaagctgttc gtacgacgtg caggaagcga tgtcctcatg
     3481 ccagccttcg aggtattcgt agtgcggaga ggctttctca aggcgttgaa gagcgcaggg
     3541 aaagtgaggc tcatcctgtc cttccaccat atatgaagtg cacacgggga ttcgttcctg
     3601 accggtgagc acatcaagtt ttgtcaaggc gatcgaggag atgccgttca ccctgaccgc
     3661 atagcgcagc gccacaaggt caagccaccc gcagcggcgc gggcgtccgg tcgtcgcgcc
     3721 gaattccgct cccttctccc gcagcttgtt tccttcgtcg ccgcaatcct ccgtggggaa
     3781 aggcccctcg ccgacccggg tgcagtaggc ttttacaacg ccaataaccc gccccaccat
     3841 cgaagggccg actcccaggc cgacacagcc tcccgccgca acaggagaag aactcgtgac
     3901 atagggatag gttccgtgat caatatcgag cagcgtcccc tgcgcgccct caaaaagcac
     3961 gcctttgccc tcccgaaggg cctcgtcgat gatcagcgag gcgtcgccga cgtacggcgc
     4021 gagcgatttt ccccatgaga gcgcgatcga gtacatttca tgaaacggaa gaggctcctc
     4081 gccgtatatt ttggagaggt acgtattttt ctcgtcaagg ttgtattcca gcttctctct
     4141 gaggacgtcg ccgtccagga gatcttccac ccggattccg cagcggctgt acttgtcgac
     4201 gtacgctggg cctattcctc tcccggtcgt gccgattttc ctccccttgc cccgaagctc
     4261 ttcctgggcc ttgtccagca ctttgtgata gggcatcacg acatgcgccg cgccgcttat
     4321 caccagccgg gcgcggtctt tcccctggtc ctgaagatcc ttgagctctt tcagaagctg
     4381 ctcggggtcg atgaccaccc cgttgccgat gatgcaaatc ttgcacgggt acaacatccc
     4441 cgacggcaaa aggtggaaga cgtatttctg cccctcgacg attactgtat gccctgcatt
     4501 cgcccctccc tggtagcggg cgaaaatatc gacctttgca ccaagggcgt caacaacccg
     4561 tccttttcct tcatcgcccc actgggcgcc gataataacc tcgactcttc ctttcacagt
     4621 acagcctcct caggtagtgc cacgttcttg cggaaaaaat ccgcgctgct cctctcgctt
     4681 cacaagcaat cagcgtatcc ggagcagatg gaacaaatcc ggaagggaga cgaatatgta
     4741 gccgtccgga agcgctcctt ccaacttttt cagatattgc agcgtcgcga tcctcgtgtg
     4801 cgcaatgacg acaacccacc cgcgccgttt cgcaagagcg agcgcacgga gaaactgcgt
     4861 ttccatgaac gtctcgtccg cctgatgatc caggaagaca gaagaataga gagccggcac
     4921 gcccttttcg acggcctttg cataggccgc ggaagatgcg cttgtccggc tgtcgagaaa
     4981 aaacaaagaa aaaccgctca atatctccat aaaagagtcc atgagctctt cgtccgcagt
     5041 cgccctcgat cccctgtggt tgttgacccc cacggcgtcc ggcagtactt caacggcat
//
LOCUS       DAOTIL010000081         5089 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminobacteriaceae bacterium isolate AMR_MDS_3026
            NODE_19462_length_5089_cov_3.823600, whole genome shotgun sequence.
ACCESSION   DAOTIL010000081 DAOTIL010000000
VERSION     DAOTIL010000081.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868050
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminobacteriaceae bacterium (wastewater metagenome)
  ORGANISM  Aminobacteriaceae bacterium
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae.
REFERENCE   1  (bases 1 to 5089)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5089)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:19:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,224
            CDSs (total)                      :: 3,198
            Genes (coding)                    :: 3,184
            CDSs (with protein)               :: 3,184
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5089
                     /organism="Aminobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19462_length_5089_cov_3.823600"
                     /isolate="AMR_MDS_3026"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:3034609"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(187..468)
                     /locus_tag="P5201_02715"
     CDS             complement(187..468)
                     /locus_tag="P5201_02715"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRV97475.1"
                     /translation="MDIVTKIRNLCSSEVDWVKDAGKAFADSADDLFDLFAERKAPSP
                     KFKVRLARTFSDVNSALAACGMEKIEGESEREIAAAYFAYCKGVIESRM"
     gene            900..1121
                     /locus_tag="P5201_02720"
     CDS             900..1121
                     /locus_tag="P5201_02720"
                     /inference="COORDINATES: protein motif:HMM:TIGR03831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system MqsA family
                     antitoxin"
                     /protein_id="HRV97476.1"
                     /translation="MKCHVCGAGMQEMKTDLPFKINERSIIVVRDVPVIQCSGCREYM
                     LSDEVMERLDELLAGVNSNADLEIVRFAA"
     gene            1232..1561
                     /locus_tag="P5201_02725"
     CDS             1232..1561
                     /locus_tag="P5201_02725"
                     /inference="COORDINATES: protein motif:HMM:NF025442.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4258 domain-containing protein"
                     /protein_id="HRV97477.1"
                     /translation="MKSDAPTEALRRIQECVKRRDILWTYHVFMRMQGRCIPRSSILD
                     SVASYEIIESYPEDKYMASYLVRAECGADVVHVLFAFDIADKFVRIITTYRPDVALWS
                     EDGRKRQ"
     gene            complement(1797..2021)
                     /locus_tag="P5201_02730"
     CDS             complement(1797..2021)
                     /locus_tag="P5201_02730"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRV97478.1"
                     /translation="MLLFLFSPEAGGAGKTGGLFAAQRSQSGEKALLRRAEDVETFPR
                     EDVRKGEPDGGERCTSSPSGLCTATNRSSA"
     gene            complement(2087..3091)
                     /gene="asrC"
                     /locus_tag="P5201_02735"
     CDS             complement(2087..3091)
                     /gene="asrC"
                     /locus_tag="P5201_02735"
                     /inference="COORDINATES: protein motif:HMM:TIGR02912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016002 - sulfite reductase activity
                     [Evidence IEA];
                     GO_process: GO:0006790 - sulfur compound metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite reductase subunit C"
                     /protein_id="HRV97479.1"
                     /translation="MTLDLNRKKVTKNAFRITKVRGATAIRVRVPGGHLDAELMKTIA
                     QIAERFGDGTLHITIRQGFEIPNIPFERMPEVNAALAPIIERLELPHGVKIESPSDGY
                     PAAGMRNISACIGARVCPFANTDTTALAQKLEALVYPNDFHVKIAVTGCPNDCVKAHM
                     QDFGIIATCRPEYDYDQCIGCEACVDACRQKVTGALRMQDGKVVKDDRRCIGCGECVL
                     ACPVAAWTRPPVTLYRLLIMGRTGKKNPRLAMPFLDRVTEEVVLKVIKNTLDYVDKHI
                     LRSLVKEHIGYIVDRTGYPVFRDAVLEGVQLNPEARIAKTMHWPGYYTLDDGNMKQAE
                     "
     gene            complement(3105..3899)
                     /gene="asrB"
                     /locus_tag="P5201_02740"
     CDS             complement(3105..3899)
                     /gene="asrB"
                     /locus_tag="P5201_02740"
                     /inference="COORDINATES: protein motif:HMM:TIGR02911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0016002 - sulfite reductase activity
                     [Evidence IEA];
                     GO_process: GO:0006790 - sulfur compound metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic sulfite reductase subunit AsrB"
                     /protein_id="HRV97480.1"
                     /translation="MKENPYRPFPAKITGIKAETDCEFTLTVAASVAMTPGQFFQVSL
                     PKIGEAPISISGFGPDWIQFTVRAVGRLTNALQELRTGENLFIRGPYGTGFPLDTFRG
                     SNLAIVAGGSGTAPVRPLIRGALDGSLPVRSLRVIAGFKSKESLLFADEFDEWRTKGE
                     VLVTIDKPQEGWDGPTGLVTEHIRAMMLDDPADVQFVVVGPPVMMKFAVAEVRLLGVP
                     EDNIWVSFERLMSCGLGKCGHCKIDSAYICLDGPVFRFSRAAGLID"
     gene            complement(3896..4918)
                     /gene="asrA"
                     /locus_tag="P5201_02745"
     CDS             complement(3896..4918)
                     /gene="asrA"
                     /locus_tag="P5201_02745"
                     /inference="COORDINATES: protein motif:HMM:TIGR02910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0016002 - sulfite reductase activity
                     [Evidence IEA];
                     GO_process: GO:0006790 - sulfur compound metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic sulfite reductase subunit AsrA"
                     /protein_id="HRV97481.1"
                     /translation="MSSFSFSLEEFSRVFDALGELYALSGPKRFPGKGRFSGTDLVKY
                     APLVSFDELELDEKSSMSPKSLVFPPDETTFYFNGDEFTEAPLEDERETVVFLRPCDI
                     NGFRRLDAIFLENGGTKDPYYLRRREKLHFFMIECATSFENCFCVSMGSNVAGEYAVA
                     LRFGQDSVLVQVKDERFAGLFASGAPSEFTPEFVRENARTVEVPDVDEMPPELFLHPL
                     WDEYDARCIACGRCNTSCVTCTCFSTRDIFYADSRRSGERRRVWDGCHLDGFTDMAGG
                     HSFRKKKGERMRFKTFHKIYDFKKRFGYNMCVGCGRCDDVCPEYISFAECINKVAAAL
                     KGGDAK"
BASE COUNT         1002 a         1512 c         1519 g         1056 t
ORIGIN      
        1 ccttatcccg ggcaacaagc cgaacattga cactgcggaa tccgggacgc ttggttttcg
       61 taacgcgcgc acccataaaa ccacctcctc cacagggaat aataacgcca taagcgttac
      121 aagtcaaaaa ttcataaatg ccttttcgtt cgcattactt tgtggtggtt cttccagatg
      181 cgcaacctac attctgctct cgatgacacc tttgcaatag gcgaagtacg cggcggcgat
      241 ctcgcgttcc gattctccct ctattttttc catgccacag gcagcaagcg cgctgttcac
      301 atccgagaaa gttcgcgcca acctgacctt gaacttcggg gacggcgcct ttcgttcggc
      361 gaaaagatcg aacaggtcgt ccgccgaatc cgcgaacgct tttccagcat ccttcaccca
      421 gtcgacttcg gatgaacaga gatttcgtat cttggtgacg atatccatta ctctcctcct
      481 ttcgacgaag caatagcatt cgtacgcaaa acctctccgg gtaaggggct ggatttgggg
      541 ttggtccaat agccgcacaa tggattgtcc agctccctcg cccatctgct ttctgagaaa
      601 catagacctg tatcttggaa aaagagcccc cttccgctcc cccttcgcaa gtccacgggc
      661 gagtctcgcc gctcggaatc gatagggttg cgaatcgttc tgcacgggat cgctgttcgt
      721 gtctcctacg gtccgaaagg ctgttttctc gatgatataa tatgataaca attttgtggc
      781 aaccattcgc aagccgatga gaaaactgat ttctgacgaa cggaatactt tcgcctcgag
      841 aaggcgctcc atgtctgacc agaacgttgc gccggcgaaa atggacggag ggaaatacga
      901 tgaaatgtca tgtttgcgga gccgggatgc aggagatgaa aaccgacctc ccttttaaga
      961 tcaatgaacg ttccatcatt gtcgttcgcg atgtcccggt gattcagtgt tccggatgcc
     1021 gggaatacat gctttccgac gaggttatgg agcgcctcga cgagctgctc gccggcgtga
     1081 actccaacgc cgacttggag atcgttcgtt tcgctgcttg aaacggaatg ttgaataatg
     1141 tcggagcctg ttatagcgta acacgggcaa agcaacctct tcggggtgct tcacaaaatg
     1201 cttttcttct tattccggaa gggtttttca aatgaagagc gatgctccaa cagaagcgct
     1261 tcgacgaatt caagaatgtg tgaaacggag agatatcctc tggacatacc atgtgtttat
     1321 gcgtatgcaa ggccgttgca taccacgaag cagcattctt gactccgttg cttcttacga
     1381 aataatagaa agttatccgg aggataagta tatggccagc tatcttgtac gagccgaatg
     1441 cggcgcggat gtcgtgcatg ttctctttgc attcgatatc gcggacaagt tcgtccgcat
     1501 tataacgacg tacagaccgg atgtggctct ctggtccgaa gacggcagga agaggcagta
     1561 agttaaaata aagttaacca gcatgaacgc gcttttttca agtatctctc cgctgtttca
     1621 ggaacacatc ctgtgaatag aaatgttcgc ttttaccgac gatatgcgtg gtacgatgtt
     1681 ctccgcgcac gcgaacatcg cccggcggta tcgggtattc acgctgcgga aggagagggc
     1741 agagtgaacg aagcaacgac gacagcgcgc atgactgcgg agccgctccg cctgctctac
     1801 gcggatgagc ggttcgtcgc ggtgcacaag ccggaggggc tgctggtgca ccgctcgccg
     1861 ccgtccggct cgcccttccg cacatcttca cgggggaacg tctcgacatc ttctgccctc
     1921 cggaggagag ctttctcgcc gctctgcgag cgctgggcgg cgaagagacc gccggtcttg
     1981 ccggcacccc cggcctccgg ggagaataaa aagaggagca ctccggccgg aaccggcgct
     2041 cccctctttt gtgtggttat agactccgcg cggaatcgtc cgaccgctac tccgcctgct
     2101 tcatattccc gtcgtccagc gtatagtacc ccggccagtg catcgtcttg gcgatccgcg
     2161 cctcggggtt cagttgcact ccttcgagca ccgcgtcgcg gaagacgggg taccccgtcc
     2221 tgtccacgat gtagccgatg tgctccttga cgaggctccg caggatgtgt ttgtccacgt
     2281 agtcgagggt gtttttgatc accttgagga cgacctcctc cgtcaccctg tcgaggaagg
     2341 gcattgcgag gcgcggattc ttctttccgg tgcgccccat gataagcagc cggtagagcg
     2401 tgacgggagg gcgcgtccac gcggccacgg ggcaggccag gacgcactcg ccgcagccga
     2461 tgcaccgccg gtcgtccttg accaccttgc cgtcctgcat cctgagcgct cccgtcacct
     2521 tctgcctgca cgcgtcgacg cacgcctcgc agccgatgca ctggtcgtag tcgtactcgg
     2581 gcctgcacgt ggcgatgatg ccgaagtcct gcatgtgcgc cttgacgcag tcgttgggac
     2641 agccggtaac ggcgatcttg acgtggaagt cgttggggta gacgagcgct tcgagcttct
     2701 gcgcgagggc agtcgtgtcg gtgttggcga aggggcagac gcgcgcgccg atgcacgcgg
     2761 agatgttccg catccccgcc gcggggtagc cgtccgacgg cgactcgatc ttcaccccgt
     2821 gcggcagctc aaggcgttcg atgatcggcg cgagcgccgc gttcacctcg ggcatccgtt
     2881 cgaaggggat gttcggaatc tcgaagccct gccggatggt gatgtggagc gtgccgtccc
     2941 cgaaccgctc cgcgatctgc gcgatggtct tcatgagttc ggcgtcgagg tgtccgcccg
     3001 gtacgcggac gcggatcgcc gttgcgccgc gcaccttcgt gatgcggaaa gcgttcttcg
     3061 tgactttctt tctgttgagg tcgagggtca tctcgcgcca cctctcagtc gatgagcccg
     3121 gcggctcggg agaagcggaa taccggcccg tccaggcaga tgtaggcgga gtctatcttg
     3181 cagtgcccgc actttccaag gccgcatgac atgaggcgct cgaaggagac ccatatattg
     3241 tcctcaggca cgcccagcag gcggacctcc gcaacggcga acttcatcat cacgggcggc
     3301 cccacgacga cgaactgcac atcggcgggg tcgtcgagca tcatcgcgcg gatatgctcg
     3361 gtgaccagcc ccgtcgggcc gtcccacccc tcctgcggtt tgtcgatcgt gacgaggacc
     3421 tcgcccttcg tgcgccattc gtcgaactcg tcggcgaaga gcaggctctc cttcgacttg
     3481 aatcccgcga tcacgcgcag agagcgcacg ggaagcgagc cgtcgagcgc gccccggatg
     3541 agcggacgaa ccggcgccgt gccggaacct ccggccacga tcgccaggtt gctcccgcgg
     3601 aacgtatcca gcgggaaacc cgttccgtag gggcctctga tgaagaggtt ctctccggta
     3661 cgaagctcct gcagggcgtt cgtgagccgc ccgacggcgc ggacagtaaa ctggatccag
     3721 tccggtccga agccgctgat cgagatgggc gcctcgccga tcttcggcag ggagacctgg
     3781 aagaactgtc cgggcgtcat tgcaacggaa gcggcgaccg tcagcgtaaa ctcgcagtcc
     3841 gtctccgcct tgatcccggt gattttggcc gggaaggggc ggtagggatt ctccttcatt
     3901 tcgcgtcccc tcctttcagg gcggcggcta ccttgttgat acactcggcg aacgagatgt
     3961 actccggaca gacgtcgtcg cacctgccgc acccgacgca catgttgtag ccgaagcgtt
     4021 ttttgaagtc gtatatctta tggaaggttt tgaagcgcat ccgctccccc ttcttcttcc
     4081 ggaaggagtg ccccccggcc atgtcggtga agccgtcgag atggcagccg tcccagacgc
     4141 ggcggcgctc gccggagcgg cggctgtccg cgtagaagat gtcccgcgtg gaaaagcagg
     4201 tgcaggtgac gcaggaggtg ttgcacctgc cgcaggcgat gcaacgcgcg tcgtactcgt
     4261 cccacagcgg gtgcaggaag agttccgggg gcatttcgtc gacgtccggg acttcgacgg
     4321 ttcgtgcgtt ctcacggacg aactcgggtg taaattcgga aggcgccccg gaggcgaaga
     4381 gaccggcgaa gcgctcgtcc ttcacctgga cgaggaccga atcctgtccg aaacgcagag
     4441 cgacggcgta ctccccggcg acgttggacc ccatggagac gcagaagcag ttctcgaacg
     4501 aggttgcgca ttcgatcatg aagaaatgaa gcttctccct gcggcgcagg tagtaggggt
     4561 ccttggttcc gccgttctcc aggaagatgg cgtcgagcct ccggaagccg ttgatatcgc
     4621 agggacgcag aaagacgacg gtctcccgct cgtcctcgag cggagcctcg gtaaattcgt
     4681 cgccgttgaa gtagaacgtc gtctcgtcgg gcgggaagac gagcgacttg ggcgacatgc
     4741 tactcttctc gtccagttcc agctcgtcga aagagacgag cggcgcgtat ttcaccaggt
     4801 ccgtgccgga aaagcgcccc ttgccgggaa aacgcttcgg cccggagagg gcgtacagct
     4861 cgcccagcgc gtcgaaaacg cgggagaact cctccagcga gaaggagaag gaggacatcg
     4921 ctacgccccc ccgttccgct cccgccggat gcggagcgcg tccgtgagcg catggaagtg
     4981 ccctatctcc tcggcgatga ggcggtcgac gtccttcgcc tccatcttgg acgagaggat
     5041 atccttcacc gcggtgtaga agaagacgga gttcttctcg acgacgatc
//
LOCUS       DAOTIL010000082         5081 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminobacteriaceae bacterium isolate AMR_MDS_3026
            NODE_19500_length_5081_cov_3.361719, whole genome shotgun sequence.
ACCESSION   DAOTIL010000082 DAOTIL010000000
VERSION     DAOTIL010000082.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868050
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminobacteriaceae bacterium (wastewater metagenome)
  ORGANISM  Aminobacteriaceae bacterium
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae.
REFERENCE   1  (bases 1 to 5081)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5081)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:19:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,224
            CDSs (total)                      :: 3,198
            Genes (coding)                    :: 3,184
            CDSs (with protein)               :: 3,184
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5081
                     /organism="Aminobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19500_length_5081_cov_3.361719"
                     /isolate="AMR_MDS_3026"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:3034609"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..501
                     /locus_tag="P5201_02750"
     CDS             <1..501
                     /locus_tag="P5201_02750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005661440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter large permease subunit"
                     /protein_id="HRV97482.1"
                     /translation="GSVWRASSTGSADSNFFNYFRNSFVVVVVSLTMILLLGSLCAYA
                     LAHWRTRISGAIYFAIIIGMIMDDVSAMLLCTPILLPVVVSLGIHPIHFAAIIGTNLG
                     LGNVTPPTAPTLYFGGRMAKTPVSQMMKPAMIFILFAWLPTLLAVTFLPELALWLPRL
                     VLGGRL"
     gene            514..1719
                     /locus_tag="P5201_02755"
     CDS             514..1719
                     /locus_tag="P5201_02755"
                     /inference="COORDINATES: protein motif:HMM:NF014381.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD/NADP octopine/nopaline dehydrogenase family
                     protein"
                     /protein_id="HRV97483.1"
                     /translation="MNMEYLKDKPVAVLGGGATARGHAACTALAGGEVRLYELPEFFE
                     GLGCIASKREIRVSGRQESLYGFKREGLAKIDVVTDDMAKAVRGAGIIVVSFPAVGYA
                     AFLERLIPHLEDGMVVHFTTANFGSLLFRKMLREAGCTKKIVVGEWSSQPYGIRIKNM
                     GGQQMPEVSVNYWAITLRGAALPMTDQDAFFESKRYLPSLDSVEHPVHGDTVADVGFS
                     NVNPILHCPGSILGVGAMENWGVVYGENKYDFSIYSHAYCPSISKVQLALYREECAIA
                     EAMGVGIQDFPEKAFFSRSNILGSEHMGEKFVVPFEEQYTLAMGTGPFSIYNRYITED
                     IPVGCHIYLELAKKFGIEVPVIESMITLASVMTGTDFRAAGVTLEDLGIAHMNKEALN
                     AWLREGIYA"
     gene            1712..3391
                     /locus_tag="P5201_02760"
     CDS             1712..3391
                     /locus_tag="P5201_02760"
                     /inference="COORDINATES: protein motif:HMM:NF013696.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HRV97484.1"
                     /translation="MPEERGSVLKKYAESGLCRLLLDMLRIPSVSPSPEHENRVTRLL
                     YDTLAVLPYFREHPEDLRLLSLEDDPFGRNFLFAFVRRPGPTAETVILAGHADVVSAE
                     ACGELAPFAFDPEEYTRRLEDVALPEDARRDLESGEWLFGRGVADMKSGLAAGIELLA
                     EAASDPGSLGANLLALAVPDEENNSAGMLAASAQLARRQKEEGLRYRACIELEPTFAG
                     GEWALPSIYLGSIGKINTFFFCAGKETHVGEYYEGFGAAPVISRINLELEGNPDYADS
                     LDGRAYPPFGCMRQTDLRREYSATIMTRAFSFYSYLTATKLPGRILGEMRAIAEKALR
                     EAIEAHERNAESFAKMNGAGRSDAKWNSMALSYEELRRMAEAKLGAGFPGFVEEVLSR
                     TPSGADERTKAVALVESMVEACALPGPLVVFGFLPPWYPHRANLGLSEGERRVERAAR
                     ETVREASERFGLTVETRPFFEGVSDLSYCGFQGEAGEMATFAANMPGWKRLYSLPTEA
                     LAELDIPILNFGPLGKDAHKNTERLHLPYFMEVFPKLLRSLVRRVAEDGER"
     gene            3653..4198
                     /gene="pyrR"
                     /locus_tag="P5201_02765"
     CDS             3653..4198
                     /gene="pyrR"
                     /locus_tag="P5201_02765"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005662297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional pyr operon transcriptional
                     regulator/uracil phosphoribosyltransferase PyrR"
                     /protein_id="HRV97485.1"
                     /translation="MTERDMERVLRRIAVEIVERNKGLDDVVLLGIQRRGVHLAARLR
                     EIFKAEEKVKVPAGELDITLYRDDISTLADQPVVHSTSLPGDITGKRVVLVDDVLFTG
                     RTIRAALDAITDLGRPERVQLAVLVDRGHRELPIAADYVGKTIPTSRSENVEVRVKEL
                     DGTDGVVICERNGGAEHGVEA"
     gene            4182..>5081
                     /locus_tag="P5201_02770"
     CDS             4182..>5081
                     /locus_tag="P5201_02770"
                     /EC_number="2.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013048980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004070 - aspartate carbamoyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0016597 - amino acid binding [Evidence
                     IEA];
                     GO_process: GO:0006207 - 'de novo' pyrimidine nucleobase
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate carbamoyltransferase catalytic
                     subunit"
                     /protein_id="HRV97486.1"
                     /translation="MEWRHKDLISLEGWTREELHFFLEQADYMEELMNRPIKKVPALR
                     GKMVVNCFFENSTRTRVSFELAEKMLSADVVNWSASGSSTSKGETLRDTVWTLEAMGA
                     DAVVVRHGAVGVPDYLSKKLHRASVFNAGDGTNGHPTQALLDLHSARKKLGNLDGAKM
                     AMIGDVLHSRVARSDIQAFRKMGVTVTLSGPATLMPEDTGSLGVEYVSDAREAARGAD
                     ILYFLRIQRERQEDGLIPSIDEYHRRWGATDSLVALASPKAAVMHPGPMNRGVEIASS
                     VADGPNSVILGQVRSGVAVRMALL"
BASE COUNT          912 a         1505 c         1732 g          932 t
ORIGIN      
        1 ggttcggttt ggcgcgcgtc cagcacgggt tcggcggaca gcaacttctt caattacttc
       61 cgcaacagct ttgtggtggt ggtggtctcg ctgacgatga tcctgctgct cggttccctg
      121 tgcgcctacg cgctcgccca ttggcggacg aggatctccg gcgcgatcta cttcgcgatc
      181 atcatcggca tgatcatgga cgatgtgagc gcgatgctgc tctgcacccc gatactgctt
      241 ccggtcgtgg tctccctggg gattcacccc atccatttcg ccgcgatcat cgggacgaat
      301 ctcggcctgg gaaacgtgac gccgccgact gcgccgacgc tctacttcgg cgggcgcatg
      361 gcgaagacgc ccgtttcgca gatgatgaaa ccggccatga tcttcattct tttcgcatgg
      421 ctgccgacgc ttctggcggt gaccttcctg ccggaacttg cactctggct gcctcgtctt
      481 gtgctcggcg gccggctcta ggaggcgtcc gatatgaata tggagtattt gaaagacaaa
      541 ccggtagcgg ttctcggcgg cggggcgacg gcgcgcggtc atgccgcctg tacggcgctc
      601 gccggggggg aggtccgcct ttacgaactg cctgagttct tcgaggggct ggggtgtatc
      661 gcgagcaaac gcgaaatacg agtcagcgga aggcaggaga gcctgtacgg attcaagcgc
      721 gagggactcg cgaagatcga cgtggtcacc gacgatatgg cgaaggccgt ccgcggcgcg
      781 gggattatcg ttgtcagctt ccccgccgtg gggtacgcag cctttcttga gcgtctgatc
      841 ccgcaccttg aggacggaat ggtcgtgcat ttcacgacgg cgaacttcgg ctcgctgctc
      901 ttccggaaga tgctccgcga agccggatgc acgaagaaga tcgttgtcgg cgagtggagc
      961 agtcagccct acggcattcg gatcaagaac atgggaggcc agcagatgcc cgaggtgagc
     1021 gtcaactatt gggcgatcac gctgcgcggg gcggcgctgc cgatgacgga ccaggatgcc
     1081 ttcttcgaat cgaagcgcta cctcccgtcg ctcgactccg tggagcaccc cgttcatggg
     1141 gataccgtcg cggacgtcgg tttcagcaac gtcaacccta tcctgcactg cccggggtcc
     1201 atcctcggcg tcggcgcgat ggagaactgg ggcgtcgtct acggtgagaa caagtacgat
     1261 ttctccattt actcgcacgc atactgccct tcgatctcga aggtgcagct cgccctctac
     1321 cgcgaagagt gcgccatcgc cgaagcgatg ggtgtcggca ttcaggactt cccggagaag
     1381 gccttctttt cgaggtcgaa cattctgggg tcggaacaca tgggggagaa gttcgtcgtc
     1441 cccttcgagg agcagtacac gctggcgatg ggaacggggc cgttctccat ctacaaccgc
     1501 tacatcaccg aggatattcc cgtcggctgt cacatctatc tggagctggc gaaaaagttc
     1561 ggaatcgagg ttcccgtcat cgagtccatg atcacgctcg cctccgtcat gaccgggacg
     1621 gacttccggg ccgccggcgt gacgctggag gacctgggaa tcgcccacat gaacaaagaa
     1681 gcgctgaacg cctggcttcg ggagggaatc tatgcctgag gagaggggga gcgttctgaa
     1741 aaaatacgcc gagagcgggc tctgccgtct gctgctcgac atgctgcgga ttccgagcgt
     1801 ctccccctcg ccggagcatg aaaaccgcgt gacgcgcttg ctgtacgaca cgctcgccgt
     1861 gcttccgtac tttcgggagc accctgagga tctccgcctc ctttcgcttg aggacgaccc
     1921 gttcggacga aatttccttt tcgcattcgt acgcaggccg gggccgacgg cggagacggt
     1981 cattctggcg gggcacgcgg atgtggtgag cgcagaggcg tgcggcgagc ttgccccctt
     2041 cgcgttcgac ccggaggagt acacgcggcg tctggaggac gttgcgcttc ccgaggacgc
     2101 ccgtcgcgac ctcgaaagcg gggagtggct cttcgggcgg ggcgtcgccg atatgaagag
     2161 cggacttgcg gcgggtatcg aactcctggc ggaggcggcg tcggatcccg gaagtctcgg
     2221 cgcgaatctg ctcgcgctcg cggttcccga cgaggagaac aactccgcag gaatgctcgc
     2281 cgcatcggcg cagctggcgc gcaggcaaaa ggaagagggg ctgcgctacc gcgcgtgcat
     2341 cgaactcgaa cccactttcg caggggggga gtgggcgctt ccgtccatct accttggcag
     2401 catcgggaag atcaacacct ttttcttctg cgccggaaag gagacgcacg tcggggagta
     2461 ctacgagggg ttcggcgcgg cgccggtgat ctcccggatc aacctggagc ttgaaggcaa
     2521 cccggactat gcggattcgc tcgacgggag agcgtacccc ccgttcggct gtatgcgcca
     2581 gacggacctt cggagggagt actcggcgac gattatgacg cgcgccttct ccttctacag
     2641 ctacctcacg gcgacgaagc tgccggggcg catcctcggc gaaatgcgcg ccattgcgga
     2701 gaaggcgctg cgggaagcga tagaggcgca cgagcgcaac gccgaatcgt tcgcgaagat
     2761 gaacggggcg ggacgctccg acgcaaagtg gaattccatg gccctctcct acgaagagct
     2821 gcgccgcatg gcggaggcaa aactgggcgc ggggttcccc ggcttcgtcg aagaggttct
     2881 ttcccggacg ccttccgggg ccgacgaacg gacgaaggcc gtcgcgctcg tcgaaagcat
     2941 ggtcgaggcg tgcgcgctgc ctgggccgct tgtcgtcttc ggtttcctgc cgccgtggta
     3001 ccctcaccgc gcgaacctcg gcctttcaga aggggagcgc agggtagagc gtgcggcgcg
     3061 cgaaacggtg cgggaggcca gcgagcggtt cgggctgacg gtggaaacgc gcccgttctt
     3121 cgagggggtt tccgacctca gctactgcgg ttttcagggc gaggcggggg agatggcgac
     3181 gtttgcggcc aacatgcccg gctggaaacg gctctacagc ctgccgacgg aggcgcttgc
     3241 ggagctggac atcccgatct tgaacttcgg cccgctcggg aaggacgccc ataagaatac
     3301 ggagcgtctg caccttccgt acttcatgga ggtctttccg aagctcctgc gttctctcgt
     3361 caggagagtt gcggaagatg gagaaaggta actttcttcc tcgatttttt ctctgcaacc
     3421 gcttgcatag attatgagag tgtggtatca ttcccccagc ttgaagagag gggggaggcg
     3481 ccatgtggag tactatctgt cggccgcgtg ttcgcagcgc ttctcttcct gttctcggac
     3541 acaatgtcgg agcctttctc ccgtcgggag aggggctctt tttttacgga agggagatgg
     3601 gatcgtggtg aaagcgcagt ccggaaatgt gtggacggaa aaagcgacgg tcatgacgga
     3661 gagggatatg gagcgggtgc tccggcgcat cgccgtcgag atcgtcgagc ggaacaaggg
     3721 gctcgacgac gtggtgcttc tgggaatcca gcggcgcggc gtgcatcttg cggcccgctt
     3781 gagggagatc ttcaaggccg aagagaaggt gaaagtccct gcgggcgagc tggatatcac
     3841 gctctaccgc gacgacatct cgacgcttgc cgaccagccc gtggtgcaca gcacctcgct
     3901 ccccggcgac atcacgggga agcgcgtggt gctggtggac gacgtcctct tcacggggcg
     3961 gaccatccgg gcggcgctcg acgcgatcac cgacctgggg cgtccggagc gcgtgcagct
     4021 cgccgtgctg gtggaccggg ggcacaggga gctgcccata gccgcggact acgtcggaaa
     4081 gacgatcccc acgtcgcgct cggagaacgt cgaggtgcgg gtgaaggagc tcgacggcac
     4141 ggacggcgtt gtcatctgcg aacggaacgg aggtgcggag catggagtgg aggcataagg
     4201 acctcatcag cctcgaaggc tggacgaggg aggagctgca cttcttcctg gagcaggcgg
     4261 actatatgga agagctgatg aaccgtccga tcaagaaggt ccccgcgctg cgcggcaaga
     4321 tggtggtgaa ctgcttcttc gagaactcga cgcggaccag ggtctccttc gagctggcgg
     4381 agaagatgct ctccgcggac gtggtgaact ggtcggcctc cggatcgagc acgagcaagg
     4441 gcgagacgct gcgcgacacg gtctggacgc tcgaagcgat gggcgcggac gctgtggtgg
     4501 tgcgccacgg cgcggtgggc gtccccgact acctctcgaa aaagttgcac agggcctctg
     4561 tcttcaacgc gggggacggg acgaacggac atccgaccca ggcgctgctc gacctgcaca
     4621 gcgcccggaa gaagctcggc aacctcgacg gcgcgaagat ggccatgatc ggcgacgtgc
     4681 tccacagcag ggtggcgcgc tccgacatcc aggccttccg gaagatgggc gtcacggtga
     4741 cgctcagcgg accggcgacg ctgatgcccg aagataccgg ttcgctcggc gtcgagtatg
     4801 tgtccgacgc gcgcgaagcg gccagggggg ccgacatcct ctacttcctg cggatacagc
     4861 gcgagcggca ggaggacggg ctcatcccct ccatcgacga gtaccaccgg cgctggggcg
     4921 cgacggactc gcttgtcgcc ctcgcctcgc cgaaggccgc ggtgatgcat ccggggccga
     4981 tgaaccgcgg agtggagatc gcctcttccg tggccgacgg cccgaacagc gtgattctcg
     5041 gtcaggtccg gagcggcgtc gccgtccgga tggccctgct c
//
LOCUS       DAOTIL010000083         5068 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminobacteriaceae bacterium isolate AMR_MDS_3026
            NODE_19579_length_5068_cov_3.410932, whole genome shotgun sequence.
ACCESSION   DAOTIL010000083 DAOTIL010000000
VERSION     DAOTIL010000083.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868050
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminobacteriaceae bacterium (wastewater metagenome)
  ORGANISM  Aminobacteriaceae bacterium
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae.
REFERENCE   1  (bases 1 to 5068)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5068)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:19:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,224
            CDSs (total)                      :: 3,198
            Genes (coding)                    :: 3,184
            CDSs (with protein)               :: 3,184
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5068
                     /organism="Aminobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19579_length_5068_cov_3.410932"
                     /isolate="AMR_MDS_3026"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:3034609"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..243
                     /locus_tag="P5201_02775"
     CDS             <1..243
                     /locus_tag="P5201_02775"
                     /inference="COORDINATES: protein motif:HMM:NF022321.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2703 domain-containing protein"
                     /protein_id="HRV97487.1"
                     /translation="LEVERPIEEVLNAGVTFERCENCSLADGTPFAMRTLKTEGSSFA
                     ALPPGILSDALIRVVFSTMGSCGKGSCASCAGCGEH"
     gene            263..796
                     /locus_tag="P5201_02780"
     CDS             263..796
                     /locus_tag="P5201_02780"
                     /EC_number="2.5.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014163648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008817 - corrinoid adenosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cob(I)yrinic acid a,c-diamide
                     adenosyltransferase"
                     /protein_id="HRV97488.1"
                     /translation="MSEVWITTKGGDKGETSIGNGERVPKDHARVETYGTLDECQAHI
                     GMARSLCEYPAIRDYLCLLEKEMSLLMGYIALYPGLTCPEITRLEEITHVVGEVTGKT
                     FRFVRPGDSTPGAALHIARTVARRAERIAVKLFRTGELAEDAYQYVNRLSDVIYALSL
                     WYDHLERERRGEDISCL"
     gene            880..1578
                     /locus_tag="P5201_02785"
     CDS             880..1578
                     /locus_tag="P5201_02785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006301415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HRV97489.1"
                     /translation="MESNSLSPAMNQGLRQIVYEKLKEAIVDGTIKPGSKLSEIELAE
                     RMAVSRTPVREAIRQLAQTGLVTLTPRRGAFVTMPTLKDASDLYELRTELEILAVANL
                     CSLVHSSELIDDLHRFRDVFSAMTNNTSAEKYVNEDKAFHAMIYQAVTNRFLAMMLHN
                     ISDLIHLCRPFSVENSPITVFTWGHLAIIDAILEGDLEKAKEETRKHIVVSKEGLLSF
                     LKNRASKNGEVAEA"
     gene            complement(1601..1765)
                     /locus_tag="P5201_02790"
     CDS             complement(1601..1765)
                     /locus_tag="P5201_02790"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRV97490.1"
                     /translation="MEKKYKEKARMNKKTEAPKRLVPSADFLQRKKKALQKTAAIKNP
                     GGVTKAPRAL"
     gene            1845..2372
                     /locus_tag="P5201_02795"
     CDS             1845..2372
                     /locus_tag="P5201_02795"
                     /inference="COORDINATES: protein motif:HMM:NF014724.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcF family protein"
                     /protein_id="HRV97491.1"
                     /translation="MVLVLSGGVWTNGEDFQMSAETLQRFVAGVRAADALGCALLFSG
                     GFPGRATEGQIYEMVARAAGDMKYNGPLLVEGTSRTTWENMKFSAPYIKELKAEDVVL
                     VTSAYHIKRSLFFAGRFFKGYRIHPYPSGRLSELGKVTVMDFLPSPASFQYISAAFRE
                     LVGMAVYRLLPEQSE"
     gene            complement(2379..3653)
                     /locus_tag="P5201_02800"
     CDS             complement(2379..3653)
                     /locus_tag="P5201_02800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013047843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replication-associated recombination protein A"
                     /protein_id="HRV97492.1"
                     /translation="MISSRRWEAPLAERMRPRILDEFSGQEHLLGKDAPLRKNLESSV
                     VPSCILYGPPGVGKTTLVRLMAAITSREILEINAVSAKVAELRDLVKEAENLKTLQSG
                     RSAIAFVDEIYHFNRSQQNALLPSVEKGDIILVGTTTENPWFEINKTLLSRMVVFQLN
                     PLSETDLSELLRRALADDERGLGQLGIQTDDDALRTLASMAGGDARQALTRLEFICSA
                     VAARGGSALTREFILETLPLAAVRYDKSSDDHYAVISALIKSIRGSDPDAAVYWLARL
                     LSGGEDVRFICRRLLISAAEDIGLADPQALVVASAATQAVEMTGMPEARIILSEAVLY
                     LAAAPKSNSAYLAVSAAMKGVESGDLQSVPFHLRPDGSGYIYPHDDPRHWVPQQYMTE
                     QKRFYFPGDLGSERSIGERLRKFWRRFSTHSE"
     gene            complement(3650..4609)
                     /locus_tag="P5201_02805"
     CDS             complement(3650..4609)
                     /locus_tag="P5201_02805"
                     /inference="COORDINATES: protein motif:HMM:TIGR01509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family phosphatase"
                     /protein_id="HRV97493.1"
                     /translation="MLQQRRSHHWNPALCRAFVLDWDGVLAETKLNFSPIREKFFQGR
                     FVPLFEAIATLPQPLAEELSKDIYDVEMAGAADAKPVPGALELLNWLESNKIPWCVVS
                     RNCADSILLAAQRANLPLPPLLFSRDTPPVKPAPEALWRAAEALDVPAADCVMIGDFL
                     YDLVGARRAGMRALLVQRPQAEWKHWADATFDTLTEFVEYLRAPTPLVPWEYHHIASQ
                     EGGETALELLGKYVFSISSSSIDILPLCLEQASKGVLNFFIEGEGNLTTEQWFNLAGL
                     SPVWLDQPVKEVLCHLLHARYPLARLVDDTDDFLLLPETVMVQ"
BASE COUNT         1210 a         1297 c         1354 g         1207 t
ORIGIN      
        1 cttgaagtgg agcgcccgat cgaggaggtt ctgaacgcag gagttacttt tgaacgctgt
       61 gaaaactgtt ctctagcaga cgggactccc tttgcaatga ggacgctcaa gactgagggc
      121 agttcgttcg cggcgcttcc tcccggcata ctcagcgatg ctctgatacg cgtggtcttt
      181 tccacaatgg gcagctgcgg aaaagggagc tgcgcttcat gcgccggatg cggagagcat
      241 tgagaaagtc tggtttatcc cgatgagcga agtctggatc acgacaaagg gcggcgataa
      301 aggagagacc agcattggta acggcgagag agtgcccaaa gaccatgccc gggtagaaac
      361 ctacgggact ttggatgaat gccaggctca tataggaatg gcgcgttccc tgtgtgaata
      421 cccggcaatt cgtgactacc tctgccttct cgaaaaagag atgagccttc taatggggta
      481 tatagccctg tatccggggc tgacgtgccc tgaaattacc aggcttgagg agattacgca
      541 tgttgtcggc gaagtgacag gaaaaacttt tcgttttgtc cggccgggtg actcaactcc
      601 tggagccgca ctccatattg ccagaactgt cgccaggaga gcagaacgta ttgcggtgaa
      661 actctttcgc accggtgagc tggctgaaga tgcataccaa tacgtcaaca ggttatcgga
      721 cgtaatttac gcgctttccc tatggtacga tcatctggaa cgtgaacgca gaggtgagga
      781 tatttcctgc ctctaaacct gtttcaaaaa aacggggttt tgaaagcact tcccatgcag
      841 tataatgggt acattgttct gcatgggagg tcgttatctg tggaaagcaa ctcattatcg
      901 ccggcgatga atcaaggtct tcgacagatt gtctacgaaa agttgaagga agcgattgtt
      961 gacggcacga tcaaaccagg atcaaaactt tcggaaattg aactcgccga aaggatggct
     1021 gtttcgagga ctcctgtcag ggaagcaata cgtcagcttg cccagacggg gcttgtgacc
     1081 ctgactcccc gtcggggagc tttcgtcacg atgcctacct tgaaggacgc ttcggacctg
     1141 tatgaactcc ggaccgaact tgagattctg gctgtcgcga atctctgttc tcttgtacat
     1201 tccagtgaac tcatcgatga tttacatcgt tttcgcgacg tcttttctgc catgacgaac
     1261 aacacaagcg cagagaagta cgtcaacgag gataaagcgt ttcacgcaat gatctatcag
     1321 gcggtaacaa atagattcct cgcgatgatg ctgcacaaca tatccgacct tattcacctg
     1381 tgcagaccct tttctgtcga gaactcgccg atcacggttt ttacatgggg acaccttgca
     1441 attattgatg ccatccttga aggggatctg gaaaaagcaa aagaagaaac ccgcaagcac
     1501 attgtagtaa gcaaggaagg tcttctgagt ttcttgaaga atcgcgcgtc gaaaaacggc
     1561 gaggtagctg aggcttaagc atgacctggc tgtacttcat ttacaaagcc ctgggagcct
     1621 ttgttacccc tcccggattt tttattgcag ctgttttctg cagcgctttc ttttttcttt
     1681 gcaggaagtc tgctgacggt acaaggcgct tcggcgcctc tgttttttta ttcattctgg
     1741 ctttttcttt gtacttcttc tccattccct ggagcgtgaa ggttcttgtg tccccgcttg
     1801 aagatccttt cggatttact ctgccggccc ctcaggggaa aagtgtggta ttggtactct
     1861 ccggaggggt atggacaaac ggagaagatt tccagatgag cgcagaaaca ttgcaacgtt
     1921 ttgtcgccgg agtgcgggct gcagatgcgt tggggtgcgc ccttctcttc tctggaggtt
     1981 ttcccggcag agccacggaa gggcaaatct acgaaatggt tgcgcgcgca gcaggcgaca
     2041 tgaagtataa cggtccttta ctggtagaag gcacttccag gacgacgtgg gaaaatatga
     2101 aattttcggc gccttatata aaggaattga aagcggaaga cgtcgttttg gtcacgtccg
     2161 cgtatcatat aaaacggtcg ctcttcttcg cagggcgttt tttcaagggg taccgtatac
     2221 acccataccc ttccgggcgg ctttctgagc ttgggaaggt aaccgtaatg gactttctcc
     2281 cctcccccgc ttcttttcag tacatttctg ccgcgtttcg ggaactggtc ggaatggcag
     2341 tctacagact gctcccggaa cagtctgaat aataaacatt actcggaatg ggtactgaaa
     2401 cgtctccaga actttctgag ccgctctcca atacttcttt cgcttccaag atccccggga
     2461 aaataaaaac gtttctgttc cgtcatgtac tgctgcggca cccagtggcg cgggtcatcg
     2521 tgcggataga tgtatcctga gccatccgga cgaagatgga aggggacaga ttgaagatct
     2581 ccgctttcca cgcccttcat cgctgcactc accgcaagat aggcgctgtt gctcttcgga
     2641 gcggcagcaa ggtatagaac cgcttctgaa agaatgatgc gcgcttcagg cattccggtc
     2701 atttccacag cttgagttgc tgcggaggca acgacgagcg cttgagggtc tgcaagacca
     2761 atgtcctcgg ctgcggagat gagaaggcgg cggcagataa accgcacgtc ttcacctcct
     2821 gaaaggagcc gcgccagcca gtacacggct gcatcgggat cagagccgcg gatacttttt
     2881 atgagcgctg agataacagc ataatgatca tctgaggatt tatcgtacct gactgccgcc
     2941 agggggagcg tttcaaggat aaactccctg gtgagcgcag agcctccgcg cgcggccacg
     3001 gctgaacata taaattcaag gcgcgttagc gcctgacgcg catcgcctcc tgccatcgaa
     3061 gcgagtgtcc ggagggcatc atcatccgtc tgaatgccca gctgccccaa tcccctctcg
     3121 tcatcggcca gtgccctgcg cagcagctca gaaagatccg tttcggaaag aggatttaac
     3181 tgaaagacga ccatccttga gagaagcgtt ttgttgattt cgaaccatgg gttttcggtc
     3241 gtcgtaccta caaggataat atcccctttt tcgacggacg gtagaagggc gttctgctga
     3301 cttcggttga aatgatatat ttcgtccacg aaagcgatgg cgcttcttcc ggactggagt
     3361 gtcttaaggt tttccgcttc ctttacaaga tcgcgaagtt ccgcaacctt cgcggagacc
     3421 gcattgattt cgagtatctc tcgtgaggtt atcgccgcca tgagacgaac aagcgtggtc
     3481 ttccctacgc caggaggtcc gtagaggata cagcttggca cgacagagct ctccaggttc
     3541 tttcgcagcg gagcatcctt tccaagcagg tgttcctgcc ccgagaactc gtcgagtatc
     3601 cgcgggcgca ttctttcagc cagaggggct tcccaccgtc tggatgagat cactgtacca
     3661 tcaccgtttc cggcagaaga agaaaatcgt cggtatcatc gaccaatcgc gcgagaggat
     3721 atcgggcgtg cagcaaatgg cacaaaacct cctttaccgg ctgatccaac catacgggag
     3781 agaggcctgc gagattgaac cactgttctg ttgtgagatt gccttctccc tcgatgaaaa
     3841 agttgagcac gccttttgat gcctgctcca gacagagagg caaaatatcg atgcttgagc
     3901 tggatatcga gaatacatat ttcccgagca attcaagtgc cgtctccccg ccttcttgag
     3961 aggcgatatg gtggtattcc caagggacaa gaggagtcgg cgcacggaga tactctacaa
     4021 attcggtcaa tgtatcgaaa gtggcgtccg cccagtgctt ccactctgct tgtggacgct
     4081 gcacaagcag agctctcatc cctgcgcgcc tcgcgccgac aagatcatag aggaaatccc
     4141 ctatcatgac acaatcggcg gcggggacat caagcgcttc cgctgcgcgc cacaatgctt
     4201 cgggagctgg ttttacaggt ggggtgtccc tgctgaaaag caaaggaggg aggggaaggt
     4261 tggctcgttg tgcagcaaga agaatggagt cggcacaatt ccgtgaaacg acgcaccagg
     4321 ggatcttgtt gctctctaac caattcagga gttccaatgc cccggggaca ggtttcgcgt
     4381 ctgcggctcc ggccatttcc acgtcgtaaa tatctttgct caattcttca gcgagcggct
     4441 gtggcaacgt agcgatagct tcaaaaagag gtacgaagcg cccctggaaa aacttctctc
     4501 ggatcggtga aaagttcaat ttcgtttcag caagcacacc atcccagtca agcacaaatg
     4561 cacggcataa tgcgggattc caatggtgtg agcggcgttg ttggagcact ggatacccct
     4621 cctcatataa aagacagggg cgcgggcttc cccgtcccct gtagccaagc ttccccgcaa
     4681 tgtcgtgtca tgaacgtctt gacccgttcc tatagtattg gggcctgctg aaccttagtt
     4741 tcgtcatcgc acgaggcaat tcctctaccg gcccaaactg gcggccccgc aaggcgcgag
     4801 tgttggctca agacaaaaat catgatcacg ggcaccgcag ggactctttc tttcttgttg
     4861 tatattatag cacaaaaatg ggaatcgccc agtcgcaggc gattcccaaa agagagaaaa
     4921 gataaaatga ctactcgatg tctgtgagac cgtccttgaa gatgaaatag tagaacacgg
     4981 cgacaaagag aattccgccg accatattcc ccagcgtaac ggaaataagg ttgctcatgt
     5041 agccgcccag gccgacgttg gcaagcgc
//
LOCUS       DAOTIL010000084         5047 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminobacteriaceae bacterium isolate AMR_MDS_3026
            NODE_19676_length_5047_cov_3.150040, whole genome shotgun sequence.
ACCESSION   DAOTIL010000084 DAOTIL010000000
VERSION     DAOTIL010000084.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868050
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminobacteriaceae bacterium (wastewater metagenome)
  ORGANISM  Aminobacteriaceae bacterium
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae.
REFERENCE   1  (bases 1 to 5047)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5047)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:19:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,224
            CDSs (total)                      :: 3,198
            Genes (coding)                    :: 3,184
            CDSs (with protein)               :: 3,184
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5047
                     /organism="Aminobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19676_length_5047_cov_3.150040"
                     /isolate="AMR_MDS_3026"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:3034609"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..388)
                     /locus_tag="P5201_02810"
     CDS             complement(<1..388)
                     /locus_tag="P5201_02810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009200900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRV97494.1"
                     /translation="MFKAKHLREIQAFMEKTGIPGRDGWELPASKKSFPDGANYRIEI
                     AGVERYSNMVAMVDEAKKRKIPIHRAICTVSGSTYCDFDELKNMAKLARDEQIEMIMV
                     VGHRKAWDVGSKEMGYPEGGPQGPRHR"
     gene            complement(476..1150)
                     /locus_tag="P5201_02815"
     CDS             complement(476..1150)
                     /locus_tag="P5201_02815"
                     /inference="COORDINATES: protein motif:HMM:NF019349.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HRV97495.1"
                     /translation="MENVTFERDTLSERVADFIRRQILYTDVFKDGDHILETDIADHL
                     QVSRATVREALKDLETQGIVEIIPRKGTYVAAFDHKDMIEILELRCMCEAYIFETVVN
                     GDMLDARDFAVLESIIDEMAQVSRSAEESELAKSIAFTTKDLEFHGYIWKKSGRRWFC
                     KVLSDNYYRLRLAMMQDMILERDMEKSATMHYDILNALREKDLDTARNYLRRHILSLH
                     GAETVA"
     gene            complement(1247..2419)
                     /locus_tag="P5201_02820"
     CDS             complement(1247..2419)
                     /locus_tag="P5201_02820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009200907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M24 family metallopeptidase"
                     /protein_id="HRV97496.1"
                     /translation="MLLFPASEYAERLAKTKERMHEHGVEVLIVSHPANMNYLTGYDG
                     WSFYVHQGLIVSLDCDEPIWFGREQDGNAARILTSLKEENIKGYPDHYVMSKLRHPMQ
                     FVADILRERGWDARHVGVEMEGYYFTAEGFQTLRRELPKGVVKDGTLVVNLVRRIKSA
                     REIEYVRQAARIAENVMKTALDVIAPGVRENDAAAEVYKAQIAGTPEFGGDYTSLCPI
                     MPSAERTSSAHLTWCADRKYREGDIVLLELAGARHHYHCPISRTLFLGEPPAELRKTA
                     DAVVKGLNETVAFIRPGVTAEAVEEKWRESIAGTGVHKPSRVGYSFGLNYPPDWGEQT
                     LSLRPADTTILQPGMCIHFMPGIWLKDFGFECSEPLVVTGSGCELFLNFERKLFVK"
     gene            2761..3252
                     /locus_tag="P5201_02825"
     CDS             2761..3252
                     /locus_tag="P5201_02825"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRV97497.1"
                     /translation="MKKLFALAAVLALALLVSASAFAGTFTLPKAGIDFTLPDSWQPS
                     DSKLGTMFVSPGSDMVIFFGETNSEAGLDGAMELVEQVVSEVLTDIELGEDTEHDING
                     IQVFALEGTGKSDGANAFVSISILNAEGDSLGIMVSFGDEGADQKNAADLQALVGSLK
                     IAQ"
     gene            complement(3337..3939)
                     /locus_tag="P5201_02830"
     CDS             complement(3337..3939)
                     /locus_tag="P5201_02830"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRV97498.1"
                     /translation="MKRVLVVLSLFALLLFASCASASLVELKPGADELKRMSIFLSNF
                     TEVGFFEIEDVSWLTGDDLVYFGIRHNYINNFKSRIRPCKVKNCPYGSMTIEAKWVAE
                     SVKKYFDIDFKQHDDAEEPLKAHYDGKLYHFDGSDGDVVYYAQVQTVARDGDVFHFKG
                     HLYNVSDKKEIPALFRATAKAHTFGGKRTWALLSIETVWN"
     gene            complement(3980..4804)
                     /locus_tag="P5201_02835"
     CDS             complement(3980..4804)
                     /locus_tag="P5201_02835"
                     /inference="COORDINATES: protein motif:HMM:NF018684.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysozyme inhibitor LprI family protein"
                     /protein_id="HRV97499.1"
                     /translation="MFRKKQVPNAFVFRRRSPERWEYCLEVRKCEGKDDMMRKEYTVR
                     FTGVGRLARVFIAVAFVVLAAFPAFALSDADHKAMLAASQEYRDADEGLNGAWKEAKK
                     ILAADEFKKLTAEQRAWVKKGRDDEAKALISSGLTKTQAYTEATMDRVLYINNVISRS
                     VLVHSDKGYQGLYTFAGKGMTGELEVWWYDEDEPEHYVVFENSVVFGKDNVHFCSFTG
                     KGELEGDTLVAVSDIDDAISVTIVFDGKKAVVSTDDAFKESGFCGQGVLLDGVYSR"
BASE COUNT          923 a         1598 c         1411 g         1115 t
ORIGIN      
        1 ccctgtgcct cggcccctgc gggcctccct ccgggtaccc catctccttg gagccgacgt
       61 cccacgcctt acggtgaccg accaccatga tcatctcgat ctgctcgtcg cgcgcaagct
      121 tcgccatgtt cttcagctcg tcgaagtcgc aataggtcga gccgctcacc gtgcagatgg
      181 ccctgtggat gggaattttg cgcttcttcg cttcgtccac catggcgacc atgttggagt
      241 agcgctccac tccggcgatc tcgatgcggt agttcgcccc gtcggggaag ctcttcttcg
      301 aggcgggcag ctcccatccg tcccttcccg gaatccccgt cttctccatg aacgcctgaa
      361 tctccctgag gtgtttcgcc ttgaacatcc ctgaagcgcc tccttttaat gtcgtcattt
      421 ttttgtcgac acactctccg ccggtaaaaa aagcccgcac ggggcaggct cgtcgtcagg
      481 cgaccgtttc ggcgccgtgg agcgagagaa tgtgtctgcg aaggtagttt ctggccgtgt
      541 ccaggtcctt ctccctgagc gcgttcagaa tatcgtagtg catcgtggcc gacttctcca
      601 tgtcccgttc gaggatcatg tcctgcatca tggccagccg cagcctgtag tagttgtccg
      661 agagcacctt gcagaaccag cgccggccgg acttcttcca gatgtagcca tggaactcaa
      721 ggtccttcgt ggtgaaggcg atcgacttgg cgagctcgct ctcctccgcc gagcgggaga
      781 cctgcgccat ctcgtcgatg atcgattcga ggacggcgaa gtcgcgcgcg tcgagcatgt
      841 ccccgttcac gaccgtctcg aagatgtacg cctcgcacat gcagcggagt tcgaggatct
      901 cgatcatgtc cttgtggtcg aaggccgcga cgtacgtccc cttgcggggg atgatctcga
      961 cgatcccctg cgtctccagg tccttgaggg cttcgcggac cgtggccctg ctcacctgta
     1021 ggtgatcggc gatatcggtc tcaaggatgt ggtcgccgtc cttgaagacg tccgtgtaca
     1081 gtatctgccg ccggatgaag tcggcgaccc gctcgctcaa agtatccctt tcgaaggtga
     1141 cgttttccat gacgcgctct ccgtttcccg gggaggagat caccgctccc cgttcgttct
     1201 ctctggtcta ttgtatgtgg tccgctcttt tattctatca tttttgtcat ttgacaaaca
     1261 gttttcgctc gaagttcagg aagagctcgc acccgctccc ggtgacgacg agcggctcgc
     1321 tgcactcgaa gccgaaatcc ttcagccaga tgcccggcat gaagtggatg cacatccccg
     1381 gctggaggat ggtcgtatcc gccgggcgga ggctgagcgt ctgctcgccc cagtcgggcg
     1441 ggtagttcag gccgaaggag tacccgacgc gcgacggttt gtgcacgccc gttccggcga
     1501 tggactccct ccacttctcc tccaccgcct cggccgtgac gcccggacgg atgaaggcca
     1561 ccgtctcgtt cagccccttg acgacggcgt ccgctgtctt ccgcagctcc gcgggcggct
     1621 ctccgaggaa gagcgtgcgc gatatggggc agtgatagtg atggcgcgcc cccgcaagtt
     1681 cgagcaggac gatgtccccc tcccggtact tcctgtcggc gcaccaggtg aggtgtgccg
     1741 acgaggtgcg ctcggccgac ggcatgatcg ggcagagcga ggtgtagtcg ccgccgaact
     1801 cgggcgtgcc cgcgatctgc gccttgtaga cctcggcggc cgcgtcgttc tcgcggactc
     1861 cgggggcgat gacgtcgagc gccgtcttca taacgttctc ggcgatgcgc gccgcctgcc
     1921 ggacgtactc gatctcgcgc gccgacttga tcctgcggac gaggttgacc acgagcgtcc
     1981 cgtccttcac gacgcccttc ggaagctcgc gccggagcgt ctggaagccc tcggcggtga
     2041 agtagtaccc ctccatctcg acgccgacgt ggcgcgcgtc ccatccgcgt tcgcggagga
     2101 tgtcggcgac gaactgcatc gggtggcgca gcttcgacat aacgtagtgg tcggggtagc
     2161 ccttgatgtt ctcctctttg agggaggtga ggatgcgcgc ggcgttgccg tcctgttcgc
     2221 gcccgaacca gattggctcg tcgcagtcga gggagacgat caggccctga tggacgtaga
     2281 aggaccagcc gtcgtacccg gtgaggtagt tcatgttggc ggggtgggag acgatcagca
     2341 cttccactcc gtgctcgtgc attcgttcct tcgttttggc caggcgctcc gcgtactctg
     2401 aagccggaaa gagcaacatc ttgtcaccgc cttgtggaag ttttcgtttt tatgtcgttt
     2461 gtcgacaata aaacaatacc ccgtaaaaag cggtttgtca agacgcggga gcccgtgtat
     2521 tttttttgta tgtaacaaaa gtttttcttg ccaaagttcg cttcccgtgc tattcttccg
     2581 cttgtactgt gtttttgtac gcgtttcctg ttttctcttg aactgatgct tcaagtatat
     2641 ttgaatcgag tctatgtatc ttttgcgtaa aaaatggaag aacatggaag gaagcagctt
     2701 cgtgtcttct cgtttgagca gcacatcata ctatcattca caagggggat tcaccgtacc
     2761 atgaaaaagc ttttcgcact tgcagctgtt cttgctcttg cgcttctcgt ctccgcgtcg
     2821 gcgttcgccg gaaccttcac cctgccgaag gccgggatcg acttcacgct tcccgattca
     2881 tggcagcctt ccgactccaa gctcggcacg atgttcgtct ctcccggcag cgacatggtc
     2941 atcttcttcg gtgaaacgaa cagcgaagcg ggactcgacg gcgcgatgga gctcgtcgag
     3001 caggttgtga gcgaagtcct gaccgacatc gaactcggcg aggacaccga gcacgacatc
     3061 aacggcatcc aggtcttcgc cctcgaggga accggcaagt ccgacggcgc gaacgccttc
     3121 gtcagcatca gcatcctgaa cgccgaaggc gactcgctcg gcatcatggt ctccttcgga
     3181 gacgagggcg ccgaccagaa gaacgccgcc gaccttcagg ctcttgtcgg ctcgctgaaa
     3241 atcgcccagt aagaactaaa agcacacgga aacagcgaag gaggagccgc gaaggctcct
     3301 ccttttttat gccgtactcc accctccggg gaggggttag ttccagacgg tctctatcga
     3361 gagcagcgcc caggtcctct ttccgccgaa ggtgtgcgct ttcgccgtag cgcggaagag
     3421 ggccggtatc tccttcttgt cggagacgtt gtacagatgc cccttgaagt ggaagacgtc
     3481 tccgtcgcgg gcgaccgtct gtacctgcgc gtagtagacc acgtcgccgt cggatccgtc
     3541 gaagtggtag agcttgccgt cgtagtgcgc cttcagcggc tcctccgcgt cgtcgtgctg
     3601 cttgaagtcg atgtcgaagt acttcttcac cgactccgcc acccacttcg cctcgatggt
     3661 catcgacccg taggggcagt tcttcacctt gcaggggcgg atccggctct tgaagttgtt
     3721 gatgtagttg tgccggatgc cgaagtagac cagatcgtcc cccgtcagcc aagagacgtc
     3781 ctctatctcg aagaaaccga cctccgtgaa gttgctcaag aagatgctca tcctcttgag
     3841 ttcgtccgcg cccggcttca gctcgacgag cgaggcagaa gcgcaggacg cgaacagcaa
     3901 cagggcaaac aacgagagaa ccaccaagac tcttttcatg acacgcacct ccctgagtta
     3961 tgtaacctcc gctccccggc taccttgaat aaacgccgtc gagaagaacg ccctgaccgc
     4021 agaagccgct ctccttgaaa gcgtcgtccg tgctcacaac cgcctttttc ccgtcgaaga
     4081 cgatcgtcac agaaatggcg tcgtcgatgt cggagactgc cacaagcgtg tccccctcaa
     4141 gctctccctt gccggtgaag gagcagaagt gcacgttatc cttcccgaag accacgctgt
     4201 tttcaaaaac gacgtagtgc tcgggctcgt cctcgtcgta ccaccagact tcgagctctc
     4261 cggtcattcc ctttccggcg aaagtataca gcccctgata ccccttgtcg ctatgcacca
     4321 gcaccgaacg cgagataacg ttgttgatgt agaggacccg gtccatcgtc gcctcggtat
     4381 acgcctgcgt cttcgtcaag cccgaggaga tgagcgcctt cgcctcgtcg tcgcgtccct
     4441 tcttcaccca cgccctctgc tcggcggtca gcttcttgaa ctcgtccgca gcgaggatct
     4501 tcttcgcttc cttccacgct ccgttcagcc cctcgtccgc atcgcggtac tcctgcgacg
     4561 cggcgagcat cgccttatgg tcggcgtcgc tcagcgcaaa cgccggaaac gcagcaagca
     4621 ccacaaacgc gacggcgatg aaaaccctcg caagacgccc gacacccgtg aaccgaaccg
     4681 tatattcttt tctcatcatg tcatcctttc cttcgcactt ccgcacttcg aggcagtact
     4741 cccatcgctc cggcgacctg cgccggaaga cgaacgcgtt tggaacctgt ttttttcgga
     4801 acaaagacgc cccctcccgc cgggaaaagc ggccgcagcg caacaaagcg cgttgagggg
     4861 cgatcatccg acatgcctga attgtacagt aatgccgtgc gactgtcaaa gacacgagtg
     4921 tacacaaaag gccggaaggg acgctccata cgcggagttc cttccggcct tccatgctct
     4981 gccgaagaga gagctactcg aaggttccct tgacgacgac ctcgccctcg tccatcagca
     5041 cccttcc
//
LOCUS       DAOTIL010000085         5031 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminobacteriaceae bacterium isolate AMR_MDS_3026
            NODE_19762_length_5031_cov_3.330788, whole genome shotgun sequence.
ACCESSION   DAOTIL010000085 DAOTIL010000000
VERSION     DAOTIL010000085.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868050
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminobacteriaceae bacterium (wastewater metagenome)
  ORGANISM  Aminobacteriaceae bacterium
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae.
REFERENCE   1  (bases 1 to 5031)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5031)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:19:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,224
            CDSs (total)                      :: 3,198
            Genes (coding)                    :: 3,184
            CDSs (with protein)               :: 3,184
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5031
                     /organism="Aminobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19762_length_5031_cov_3.330788"
                     /isolate="AMR_MDS_3026"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:3034609"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..973
                     /locus_tag="P5201_02840"
     CDS             <1..973
                     /locus_tag="P5201_02840"
                     /inference="COORDINATES: protein motif:HMM:NF013974.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="3-methyl-2-oxobutanoate dehydrogenase subunit
                     beta"
                     /protein_id="HRV97500.1"
                     /translation="PEYMARRMPQVGGVFLQGESETASVCMVYGAAAMGKLAMTSSSG
                     PGISLKAEGISTLAGAQLPAVIVNVMRGGPGLGTIQAAQSDYLHATKAMGHGGFRVLV
                     LAPSTVQEAIDLTYKAFGLAVKYQNPAVVLMDGWIGSMMEPVTLPEAKEPVKNAYTGW
                     GTRYYNPSGSDVDVVTSCLLEPDRQELLNIHFGEMYETWARDEVMVEEYMLGDAEYVI
                     VAYGTAARAARTAIGSLREKGVKAGMLRPISLYPFPYDALKRLDKSRVRCILDTEMTL
                     PVQMKDDIRLGVTDAIPVHSCGRSGGVVLTNEDIEDALLRIIEEEGK"
     gene            975..1715
                     /locus_tag="P5201_02845"
     CDS             975..1715
                     /locus_tag="P5201_02845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008710451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent enzyme"
                     /protein_id="HRV97501.1"
                     /translation="MDALYERPKSLTNRATAYCPGCLHGVATRLIAELIDELDARADT
                     VAVLPVGCGTLGMFHFNTDIVVSPHGRTPAVATGVKRSAPEKLVFSYQGDGDLAAIGL
                     AEIMHAANRGENFTVIFINNGTYGMTGGQMAPTTLVGQKSTTTPEGRNPAETGYPMMM
                     CEILSQLRAPRYIARFSLDCPANVIQAKKGLRKSFELQRAGKGFTFVELLSNCPTNWG
                     LSPLECLDWMRENTMKAFPLGVFRDRED"
     gene            1718..2260
                     /locus_tag="P5201_02850"
     CDS             1718..2260
                     /locus_tag="P5201_02850"
                     /inference="COORDINATES: protein motif:HMM:NF013707.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoacid:acceptor oxidoreductase family
                     protein"
                     /protein_id="HRV97502.1"
                     /translation="MEKKITIAGFGGQGVMAIGQMLGYASCKAGGNALFLPKYGPEQR
                     GGTANCTVTLADVEIGAPESRYVDTLIALNQPSLERFLGAVRPGGLVLLNSSLCRWTG
                     GREDVKLAEIPADDIAREIGSPKVANIVVIGAYIGLSGVLTERQMLDAIEEKLGKRPE
                     LLEMNRLALKRGMESATPRT"
     gene            2491..3498
                     /locus_tag="P5201_02855"
     CDS             2491..3498
                     /locus_tag="P5201_02855"
                     /inference="COORDINATES: protein motif:HMM:NF024808.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HRV97503.1"
                     /translation="MNLKRIVVCALMTALVASYAAGTAFAAETVPLEGRMTLYTSQPE
                     RDVTKLIAAFNELHPDVAVDVFRSGTEEVVSKVRAEHAVRSVLADVLLVADSVTFESL
                     KEDGMLLPYDSPEMKGIPEEYLDKDRTYAGTKIISTGIIYNTTKVKEPPVSFADLAKS
                     EYKDSIIMPSPLYSGAAAYNLGVMTRTEGLGWEFYEALKANNIRIEKGNGAILTAVVA
                     GEKACGLVVDFMTVRSKRDGAPVEFVYPLEGSPAVTEPIGILKTTEVPELAKAFVDFV
                     LSAEGQKVAAEMGYTPVKDGVEAPEGLKSVGEIKSMVYDIAVLKEAREAEKARFSAMF
                     Q"
     gene            3514..>5031
                     /locus_tag="P5201_02860"
     CDS             3514..>5031
                     /locus_tag="P5201_02860"
                     /inference="COORDINATES: protein motif:HMM:NF012738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006826 - iron ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HRV97504.1"
                     /translation="MRVRQFAHTFGSRALKTAFYGAVFLFFIAPLLRLLLMSFAGEAG
                     YDASHYVRLLAEERTRLAVRNTAIIGTSATAFSVLFGGGFAFLVAYANLKRRRLAEVL
                     ILLPFIIPSYIITLSWTGLLSGNGAINTWLTSAGLPAVNLYSMGGIIFVHGMCATPIV
                     YMIVKAMLRRIPVDLEWASMAAGYTRRQTMLRINLALAAPAVASGAMLSFLATIDNFS
                     IPAFLGISSGIPVLSTYIYEKAIGFGPSSFSDAAVLSVLLSLLAIGGTLLEGFFVKRS
                     PGLESVGEDASTRIELTPKVRAAVEWSLLGGLFAFNIVPLLFMALSAMQKRYGLGYAA
                     DNLTVENFRALLTNAGVLRAVRNSLLLASVTCAVCIVVGTAAAYLKVRKKSGAVKVLE
                     KSASLAYAIPGIVLALSMIFHWVEPIPGVRPRIYGTIGILAVAYVTRYLVLQIKGSVN
                     AMLTVEPALEEAARAGGSGAFAVWLKILVPLLAKPVLASAFLVFIPAMTELTLSSI"
BASE COUNT          891 a         1663 c         1646 g          831 t
ORIGIN      
        1 cccggagtac atggcgcggc ggatgccgca ggtcggcggg gtcttcctgc agggagagag
       61 cgaaacagcc tccgtctgca tggtctacgg cgcggcggcc atggggaagc tcgccatgac
      121 ctcctcctcg ggaccgggga tcagcctgaa ggccgagggg atctccacgc tcgcaggggc
      181 gcagctgccc gccgtcatcg tcaacgtgat gcgcggaggg cccggactgg gcaccatcca
      241 ggcggcgcag tccgactacc tccacgccac gaaggcgatg gggcacggcg gattccgcgt
      301 gctcgtcctc gcgccgagta cggtgcagga ggccatcgac cttacctata aagccttcgg
      361 gctcgctgtg aagtaccaga accccgcagt cgtcctgatg gacggctgga tcggctcgat
      421 gatggaaccc gtgacgctcc ccgaggcgaa ggagcccgtg aagaacgcgt acacggggtg
      481 ggggacgcgc tactacaacc cctcgggaag cgacgtggac gtggtcacct cctgcctgct
      541 ggagcccgac cgccaggagc tgctgaatat ccacttcggc gagatgtacg aaacctgggc
      601 gcgcgacgaa gtcatggtcg aggagtacat gctcggcgac gcggagtacg tcatcgtcgc
      661 ctacgggact gcggcccgcg cggcccgcac cgcgatcggc tctctgcgcg aaaagggcgt
      721 caaggcgggg atgcttcgcc cgatctccct ctaccccttt ccgtacgacg ccctgaagcg
      781 cctggacaag agccgcgtcc gctgcattct ggatacggag atgaccctgc cggtccagat
      841 gaaggacgac atccgcctcg gcgtgaccga cgccattccg gtgcactcct gcgggcgctc
      901 cggaggcgtg gtgctgacga acgaggacat cgaggacgcg ctgctgcgga tcatcgaaga
      961 ggaggggaag taaaatggac gcgctctatg aacgcccgaa gtcgctgacc aacagagcga
     1021 ccgcctactg ccccggctgt ctgcacggcg tggccacccg cctgatcgcc gaactgatcg
     1081 acgagctgga cgcgcgcgcc gacaccgtcg cggtgcttcc cgtcggctgc ggcacgctgg
     1141 ggatgttcca tttcaacacg gatatcgtcg tctcgccgca cggacggact cccgccgttg
     1201 ccacgggcgt gaagcgctcc gcgccggaaa agctcgtctt ctcctaccag ggggacggcg
     1261 acctcgccgc catcggcctc gcggagatca tgcacgccgc gaaccggggg gagaacttca
     1321 ccgtgatctt catcaacaac ggcacctacg gcatgacggg cggtcagatg gccccgacga
     1381 cgctcgtcgg ccagaagtcg accacgacgc cggaggggcg caaccccgcc gagactggct
     1441 acccgatgat gatgtgcgaa atcctgtcgc agctccgcgc gccgcgctac atcgcgcgtt
     1501 tctcgctcga ctgcccggcg aacgtcattc aggcgaagaa ggggctcagg aagtccttcg
     1561 agctgcagcg cgcggggaag ggcttcacct tcgttgagct gctttcgaac tgtccgacga
     1621 actgggggct ttcgccgctg gagtgcctgg actggatgcg ggagaacacc atgaaggcct
     1681 tcccgcttgg cgtcttcagg gacagggagg actgatcatg gagaagaaga tcaccatcgc
     1741 gggcttcggc ggtcagggag tgatggccat cggccagatg ctggggtacg cctcctgcaa
     1801 agcgggcggc aacgcgctct tcctgccgaa atacggcccc gagcagcgcg gcggaacggc
     1861 caactgcaca gtgactctcg ccgacgttga gatcggcgcg ccggagtcgc gctacgtcga
     1921 cacgctgatc gcgctgaacc agccgtcgct ggagcgtttc ctcggcgcag tccgtccggg
     1981 ggggctcgtc ctgctcaaca gctcgctctg ccgctggacc ggagggcggg aggacgtgaa
     2041 gctcgccgag attccggccg acgacatcgc gcgcgagatc ggctcgccga aggtggcgaa
     2101 cattgtcgtc atcggcgcgt acatcggact gagcggcgtc ctcaccgagc ggcagatgct
     2161 cgacgccatc gaggagaagc tggggaagcg ccccgaactg ctcgaaatga accgcctcgc
     2221 gctgaagcgc ggaatggagt ccgcgacgcc gaggacgtag gacaacgtcc gtccccgcgg
     2281 acgggcttgc ggttcgccgc gccgccggga cgctcttttc cgcggcatgc agtataatgt
     2341 tgcgttgcgg gtacaccagc gcaacgcttt tgttacgttc gcgccgtaca ctttgcggga
     2401 gcgccgccgg cctccggccg atgaactcgt caggggcgaa ggcggcaaaa acccgcgaaa
     2461 cgcggactca cacgcaagga ggagaactga atgaatctga aacgaatcgt agtctgcgcg
     2521 ctgatgacgg ccctcgtcgc atcgtacgcc gccggaacgg ctttcgcggc ggagacggtt
     2581 ccgctcgaag ggcggatgac cctgtacacg tcccagccgg aacgggacgt gaccaagctc
     2641 atcgcggcct tcaacgagct gcatccggac gtcgctgtgg acgtcttccg ctcgggaacc
     2701 gaggaggtcg tcagcaaggt ccgcgcggag cacgccgtgc ggtcggtgct cgcggacgtg
     2761 ctgcttgtgg ccgacagcgt caccttcgag agcctcaagg aggacggcat gctcctcccc
     2821 tacgactccc ccgagatgaa gggcatcccc gaggagtatc tggacaaaga ccgcacctac
     2881 gcgggaacga aaatcatcag caccggcatc atctacaaca ccacgaaggt gaaagaacct
     2941 cctgtctcct tcgccgacct ggcgaagagc gaatacaagg acagcatcat catgcccagc
     3001 ccgctctact ccggcgccgc cgcgtacaac ctcggcgtga tgacccggac cgaggggctc
     3061 ggctgggagt tctacgaggc gctgaaggcg aacaacatcc gcatcgagaa ggggaacggc
     3121 gccatcctca ccgcggtggt cgcgggcgag aaggcctgcg gactcgtggt cgacttcatg
     3181 accgtccgct cgaaacgcga cggcgcgccg gtggagttcg tctacccgct tgaaggctcc
     3241 cctgcggtca ccgagccgat aggcatcttg aagacgacgg aagttcccga gctggcgaag
     3301 gccttcgtcg acttcgtcct ctcggccgag gggcagaagg tggcggcgga gatggggtac
     3361 acgcccgtca aggacggcgt tgaggccccc gaagggctga aatcggtggg cgagatcaag
     3421 agcatggtct acgacatcgc cgtgctgaaa gaggcgcgag aggccgaaaa agcgcgtttc
     3481 agcgctatgt tccagtagaa accggggaga acggtgcgcg tacggcaatt tgcacacact
     3541 ttcggaagcc gcgccctgaa gacagccttc tacggcgcgg tcttcctctt tttcatcgcg
     3601 ccgctgctcc gcctgctgct gatgagcttc gccggggagg cagggtacga cgcgtcgcac
     3661 tacgtccgcc tcctcgccga ggagcggacg cggctggctg tcaggaatac ggcgatcatc
     3721 gggacgagcg ccacggcgtt ttccgtactc ttcggcgggg gattcgcgtt cctcgtcgcc
     3781 tacgcgaacc tgaagcggcg gcgtctcgcc gaggtgctga tcctgctccc cttcatcatc
     3841 ccctcgtaca tcatcacgct ctcctggacg gggctgctct cggggaacgg cgcgatcaac
     3901 acgtggctga cctccgccgg gctccccgcc gtcaacctct actccatggg cggcatcatc
     3961 ttcgtccacg gcatgtgcgc gacgcccatc gtctacatga tcgtgaaagc catgctgcgg
     4021 cgcatccccg tcgaccttga atgggcctcg atggccgcgg ggtacacgcg ccgccagacc
     4081 atgctccgga tcaacctcgc gctcgccgcg cccgccgtgg cgagcggggc gatgctctcc
     4141 ttcctggcca ccatcgacaa cttctccatc ccggccttcc tcggcatctc ctcgggcata
     4201 ccggtgctga gcacctatat ctatgagaag gcgatcggct tcggcccctc ctccttctcc
     4261 gacgcggcgg tgctctcggt gctgctctcc ctcctcgcca tcggcggtac gctcctcgaa
     4321 ggcttcttcg tcaagcggag ccccggactg gagagcgtcg gagaggacgc ctcgacgcgc
     4381 atcgagctga cgcccaaggt ccgcgccgcg gtcgagtggt cgctgctggg ggggctcttc
     4441 gcgttcaaca tcgtcccgct gctcttcatg gcgctcagcg cgatgcagaa gcgatacggc
     4501 ctggggtacg cggcggacaa cctcaccgtg gagaacttcc gcgcgctgct gaccaacgcc
     4561 ggagtgctcc gcgctgtgcg gaacagcctg ctgctcgcct cggtgacgtg cgccgtctgc
     4621 atcgtcgtcg ggacggcggc cgcctacctc aaggtccgca agaagagcgg cgcggtgaag
     4681 gtgttggaga agagcgcctc cctcgcgtac gccatccccg gcatcgtact ggccctctcg
     4741 atgatcttcc actgggtgga gccgattccg ggggtgcgcc cgcgcatcta cgggaccatc
     4801 ggcatcctgg cggtcgccta cgtcacgcgc tacctcgtgc tccagatcaa ggggagcgtg
     4861 aacgcgatgc tcaccgtgga acccgcgctc gaggaggcgg ccagagccgg agggagcggc
     4921 gcgttcgccg tctggctgaa aattctcgtc ccgctgctgg ccaaacccgt gctggcgagc
     4981 gcgtttctcg tcttcatacc ggcgatgacg gagctgacgc tctcctccat a
//
LOCUS       DAOTIL010000086         4952 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminobacteriaceae bacterium isolate AMR_MDS_3026
            NODE_20228_length_4952_cov_3.502348, whole genome shotgun sequence.
ACCESSION   DAOTIL010000086 DAOTIL010000000
VERSION     DAOTIL010000086.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868050
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminobacteriaceae bacterium (wastewater metagenome)
  ORGANISM  Aminobacteriaceae bacterium
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae.
REFERENCE   1  (bases 1 to 4952)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4952)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:19:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,224
            CDSs (total)                      :: 3,198
            Genes (coding)                    :: 3,184
            CDSs (with protein)               :: 3,184
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4952
                     /organism="Aminobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20228_length_4952_cov_3.502348"
                     /isolate="AMR_MDS_3026"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:3034609"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..518
                     /locus_tag="P5201_02865"
     CDS             <1..518
                     /locus_tag="P5201_02865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005660288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HRV97505.1"
                     /translation="YREVQNTPNTCLFLMTFTYERKPLFKWVGPLAPTKVGVLALKSK
                     GLKITSASDLANLKAGVVRDDIGDELAKQAGIQQIERVPSNDQNIKKLTEGRIDIWVF
                     EESVAKWQIQSLGFNADDYESVWTLSESDLYYAFHKDTDDALIASIQKTLDDMKADGS
                     YEAVMKKYNMR"
     gene            701..2605
                     /locus_tag="P5201_02870"
     CDS             701..2605
                     /locus_tag="P5201_02870"
                     /inference="COORDINATES: protein motif:HMM:NF012707.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HRV97506.1"
                     /translation="MGSIRSRQSNPPFRMRVYGLLLFVFLILCAACFPTDLFAAKIVR
                     VGLYENSPKIFTAESGLPAGIFADIIRYIAEKEGWELRFVHGTWSQGLDRLEKGDIDL
                     MPDVAYTESRGKLFSFHSDPALYSWFQVFSRRGSGIRSIPDLDGKRVAVLYRSIQQEI
                     FDRIVRDFGLSVSFVTFSDFESAFQAVASGKADAVLTNRFFGAHHMRAYNLEDTAINF
                     NPTRLFFAAPQNSDPAIPAAIDKHLREMKRNPDSVYFSTLARWTSSPVFMVIPAWLKS
                     TAMLLAFLTLLGGILSFAFKRQADSRAAELTRLFDNAPMGLFFSGRDGTIKRTNAEAL
                     RMLGLESADILGEKVEDFIRRSGSKRDEKDAVTKESFSSEFHAEGKRKDGTTVPLACI
                     VFPLTPSGKMLGTYTVFQDISLQAANEKRIGSIIRELNRTVSALKKSWEQTVLVLASL
                     SEVRDPYTSGHQRRVARLAAAIACEMGMERRKARQVELAAMIHDIGKIEIPSEILSKP
                     GKLSDIEYRLIQTHPAAGHRILQTIEVPWALAEIIHQHHERMDGSGYPRGLSGSDILM
                     EARIIAVADTVEAMSSHRPYRAGKGIEAALGEVASQRGKGFAPDVADACLRLFREKEF
                     RFDEEPGRIG"
     gene            complement(2795..3382)
                     /locus_tag="P5201_02875"
     CDS             complement(2795..3382)
                     /locus_tag="P5201_02875"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRV97507.1"
                     /translation="MNPLGMIISFAPWLVLKILTSIPFSDPLTRLKTALVIAILICIY
                     QSRSAVKGFIFWGTIAFFSFSFVTVVLMTNMWVIDRLGLLSQLTMSAIAWGSILFKRP
                     FTLDYSKQYVPSEFWTHPAFLRKNYLITGVWGCYFLLGVAINEIRIYEPQISHLLLEV
                     LDNASMLAIILYTSHASKRRSVSASSENGQDTPVE"
     gene            complement(3473..4189)
                     /locus_tag="P5201_02880"
     CDS             complement(3473..4189)
                     /locus_tag="P5201_02880"
                     /inference="COORDINATES: protein motif:HMM:NF015325.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin family protein"
                     /protein_id="HRV97508.1"
                     /translation="MKILALNGSPRGAAGNTERMVQPFLEGARSAGAETEVLYVKDLR
                     IGFCKGCFACWTATPGRCVQRDDMDMVLEKMDAADTLLWATPLYHFGMTAMLKAVMER
                     TLPLADPHIVKAGDVYTHPGRSGKRFPQYLLFSNCGFPDQGHFDGLRAHFESLAKASG
                     KRGTIPAVFRAAGELLRQEEVRPHIQWYFDALRQAGKEYVEHGGISGESMEVLHRDLV
                     PQEMYISQANAHWDSVLKGK"
     gene            complement(4269..>4952)
                     /locus_tag="P5201_02885"
     CDS             complement(4269..>4952)
                     /locus_tag="P5201_02885"
                     /inference="COORDINATES: protein motif:HMM:NF024829.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family efflux transporter periplasmic
                     adaptor subunit"
                     /protein_id="HRV97509.1"
                     /translation="TAEFALRMAEFEVQQVRSSLIQGEEGGASESVRILSPVDGYVLS
                     VFEESARTVPAGTPLMEVGDPDDIEGEVDLFSVDAAVVAQEAEALIEGWGGDAPLRGR
                     VALIERSGFTKVSALGVEEQRVKVRIDFLDPVPPGYRLGDRFRIQVRIILRQNDDVLQ
                     IPLGALFRRGGDWVAFAVEEGKAVARKLEIGQNDGIYAEVLSGLSAGDRVILHPSEHV
                     SEGAEVTWE"
BASE COUNT         1109 a         1480 c         1380 g          983 t
ORIGIN      
        1 gataccgtga agtacaaaac accccgaaca catgcctttt ccttatgacg ttcacgtacg
       61 aacgcaaacc tctgttcaaa tgggtgggac cgctcgctcc gacgaaggtc ggcgtgctgg
      121 cgctgaaaag caaggggctg aaaattactt ccgcaagcga tctggccaac ctgaaggccg
      181 gggtcgtcag agacgatatc ggcgacgaac tggcgaagca ggcgggtatt caacagattg
      241 aacgagttcc ttcgaacgac cagaacatca agaagctgac cgaaggccgg atcgatatat
      301 gggtttttga agaaagcgtg gcgaaatggc agattcagag ccttggcttc aatgccgacg
      361 actacgagag cgtctggacg ctcagcgaaa gcgacctcta ctacgcattc cacaaggaca
      421 ctgacgacgc cctcatagcg tcgattcaga agacgcttga cgatatgaag gccgacggct
      481 cgtacgaagc tgtcatgaag aagtacaaca tgcggtaacc ctctctcttc cgacgacaat
      541 agggccgccc ctcaacccgg gacggtcttt tttgtccccc ccggttggta agggcgacgg
      601 ggaagaaaaa agagttactc gccctctgct cttctctttg cctttcggtg aaagtagtgt
      661 attctatgtt tgggaacaaa agaaagagga ggctggtctt atgggcagca tacgcagtcg
      721 ccagagtaat ccgccgttcc ggatgcgcgt atacggtctc cttttgtttg tatttctcat
      781 cctttgtgcg gcatgttttc cgacagacct ctttgcagcg aaaattgtcc gcgtcggcct
      841 ctacgagaac agcccgaaga ttttcactgc ggaatccggc cttcccgcag gcatttttgc
      901 ggacatcatc aggtacatag ccgaaaaaga ggggtgggag ctccggttcg tccacggaac
      961 atggagtcag gggctcgacc gtctcgaaaa gggcgatatc gaccttatgc ccgacgtggc
     1021 ctacactgag tcgcgcggaa aactcttctc gtttcactcc gaccctgcgc tgtacagctg
     1081 gttccaggtt ttttcacgcc gcggaagcgg tatccgctcg attcccgatc tcgacgggaa
     1141 acgggtcgcc gtgctctacc gatccatcca gcaggagatc ttcgaccgta tcgtccgcga
     1201 tttcgggctc tccgtatcct tcgtgacctt ttccgacttt gaaagcgcct tccaggccgt
     1261 cgcaagcgga aaggcggacg cggtgctcac caaccgcttt ttcggcgccc atcacatgcg
     1321 cgcttataac cttgaagaca ccgccatcaa cttcaacccg acgcgtctct ttttcgccgc
     1381 ccctcagaat tccgacccgg cgattccggc agccatcgat aaacacctcc gcgagatgaa
     1441 gagaaacccc gattcggtct acttcagcac gctcgcccgg tggacctcca gtcccgtctt
     1501 catggtaatc ccggcatggc tgaagtcaac cgccatgctc ctcgcttttc tgactttgct
     1561 cggaggaatc ctctccttcg ccttcaaacg gcaggccgat tcgagggccg cggagttaac
     1621 ccgcctcttc gacaacgcgc ccatgggact tttcttctcc ggaagagacg ggacgatcaa
     1681 acggaccaat gcggaagcgc ttcggatgct cggactggaa agtgcggata tcctcggaga
     1741 aaaggtcgaa gacttcatcc ggcgcagcgg tagtaaaaga gatgaaaaag acgcagttac
     1801 gaaagaaagc ttttcctcgg agttccatgc ggaaggaaag cgaaaagacg gaactacggt
     1861 tccgcttgca tgcatcgtct ttccgctgac tccttcggga aagatgctcg gaacgtacac
     1921 ggtattccag gacatctcgc tccaggcggc gaacgaaaag cggatcggct ctatcatccg
     1981 ggagctgaac agaaccgtct ccgcgctcaa aaaatcatgg gagcagaccg tcctcgttct
     2041 ggcaagtctt tcggaggtgc gagacccgta cacgtcgggg catcagcgca gggtggcccg
     2101 gctggctgcg gctattgcct gcgaaatggg catggagaga aggaaagcgc gtcaggttga
     2161 acttgccgcg atgattcacg acatcggaaa gatcgaaatc ccctcggaaa tcctctcgaa
     2221 accgggcaaa ttgagcgata tcgaataccg gctgattcag acccacccgg ctgccgggca
     2281 taggattctt cagacgatcg aggttccctg ggcgctggcg gagatcatcc atcaacacca
     2341 tgagcgaatg gacggttcgg ggtacccccg cgggctcagc ggctccgaca ttctgatgga
     2401 ggcgcgcatc atcgcggtcg cagatacggt ggaggccatg tcgtcgcacc gtccctacag
     2461 ggcggggaaa ggaatagaag ccgccctggg agaggtggcc tcgcaaagag ggaaagggtt
     2521 cgcccccgat gtcgcggacg cttgtctgcg tcttttccgg gagaaagaat tccgtttcga
     2581 cgaggaaccg gggagaatcg gctgagacgg aaaaacttac cactcctccc ggaccgcgtt
     2641 acgacggcga atagcgaaac caaaacggcg gacaaattgt ccgccgtttc atcatcatct
     2701 aaactcttct tacgctcgga gtgtattccg ataacgggga tattttatga gactttaata
     2761 aaaatggttc ctcaaacagc ataacacaca atcattattc taccggagta tcctgtccgt
     2821 tttccgacga agctgaaacg ctgcgccgct tcgaagcgtg cgacgtatag aggatgatgg
     2881 cgagcatcga ggcgttgtcg agcacttcga gaagcaggtg gcttatctgc ggctcgtaga
     2941 tccgtatctc gttgatcgcg acgcccagaa ggaagtagca cccccatacg cccgtgatca
     3001 ggtagttctt ccggagaaag gcggggtgcg tccagaactc ggacgggacg tactgtttgg
     3061 agtagtcgag cgtaaagggc cgtttaaaga ggatcgatcc ccaggcgatg gcggacatgg
     3121 tcagctgcga cagcagaccg aggcgatcga tgacccacat gttcgtcatc agcactacgg
     3181 tgacgaagct gaaggagaag aacgcgatgg ttccccagaa aataaagccc ttcaccgcgc
     3241 ttcgcgactg gtagatgcat atcaaaatcg cgatgacaag cgccgttttc agcctggtca
     3301 gcggatcgga aaaaggaatg ctggtcaaga tcttgagcac aagccacgga gcgaaggaaa
     3361 taatcatacc caaaggattc acagtacaac ctcccaagaa aatagtgtag ggccgacgga
     3421 cgaaaggcgg tacgctcgct tacggcaggg taataatagc acagtcgcct tcttatttcc
     3481 ccttcaacac cgagtcccag tgcgcgttcg cctgcgaaat gtacatctcc tgcggaacga
     3541 gatcccggtg caatacctcc attgattcgc ctgagatgcc gccgtgttcg acgtactctt
     3601 tccccgcttg gcgcagcgcg tcgaagtacc actgtatgtg cggccggacc tcctcctgcc
     3661 gtaagagctc tcctgccgcg cgaaagaccg ccggaattgt cccgcgcttc ccgctcgctt
     3721 ttgcgagcga ttcaaaatgc gccctgagac cgtcgaaatg tccctggtcg gggaagccgc
     3781 agttggaaaa gaggaggtac tgcggaaagc gtttcccgga gcggccgggg tgggtgtaca
     3841 catcgccggc cttgacgata tgcggatcgg ccaaggggag cgtccgttcc atgacggcct
     3901 tcaacatggc ggtcatgccg aagtggtaga gcggcgtcgc ccagaggagc gtatccgcgg
     3961 catccatctt ctcgagcacc atatccatgt cgtcgcgctg gacgcatctg ccgggagttg
     4021 ccgtccagca ggcgaaacac cccttgcaaa agcctattct caaatccttg acgtagagaa
     4081 cctccgtctc cgcgcccgcc gagcgggccc cctccagaaa cggctgcacc atccgttccg
     4141 tgttcccagc agcgcctctc gggctgccgt tcaaggccag aatcttcata ttttcacgct
     4201 ccttcttcag ttttctcttc cggcgcggag gatacgcgcc gacgtgtatc cggaaacctt
     4261 cgccgccatt actcccatgt cacctcggcg ccctccgaaa cgtgctccga tgggtgcaga
     4321 atcaccctgt cccccgccga cagaccggag agcacttcgg cgtagatacc gtcgttctgg
     4381 ccgatctcca gcttccgagc gacggccttt ccctcctcca cggcgaaagc gacccagtcg
     4441 ccgccgcgcc ggaaaagcgc gcccagcggt atttgcagca catcgtcgtt ctggcgcaaa
     4501 atgatgcgca cctgaatccg gaaccggtcc ccgagacgat accccggcgg cacgggatca
     4561 aggaagtcga tccggacctt cacccgctgc tcctcaacac ccagcgccga taccttggtg
     4621 aaaccgctcc gctcgatgag cgcgacgcgc ccgcggagtg gcgcgtctcc gccccatcct
     4681 tcgataagcg cctcagcctc ttgcgccacg acggcggcgt ccacggagaa gaggtcgacc
     4741 tcgccctcga tatcgtcggg atcccccacc tccatgagcg gcgtcccggc gggaacggtc
     4801 cgggcgctct cctcgaagac gctcagcacg tagccgtcca caggggagag gatgcggacg
     4861 gactcggacg ccccgccctc ctccccctgg atgagcgagg atctgacttg ctgcacctcg
     4921 aactccgcca tacggagcgc aaattccgcc gt
//
LOCUS       DAOTIL010000087         4937 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminobacteriaceae bacterium isolate AMR_MDS_3026
            NODE_20321_length_4937_cov_3.447562, whole genome shotgun sequence.
ACCESSION   DAOTIL010000087 DAOTIL010000000
VERSION     DAOTIL010000087.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868050
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminobacteriaceae bacterium (wastewater metagenome)
  ORGANISM  Aminobacteriaceae bacterium
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae.
REFERENCE   1  (bases 1 to 4937)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4937)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:19:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,224
            CDSs (total)                      :: 3,198
            Genes (coding)                    :: 3,184
            CDSs (with protein)               :: 3,184
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4937
                     /organism="Aminobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20321_length_4937_cov_3.447562"
                     /isolate="AMR_MDS_3026"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:3034609"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1017
                     /locus_tag="P5201_02890"
     CDS             <1..1017
                     /locus_tag="P5201_02890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006583425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRV97510.1"
                     /translation="ALNPTKISGICGRLMCCMSYEHTSYSSLWKTLPNPGAKIRTPAG
                     NYIVEGVELSSNSVRVRRPDGGSLLIVIEDFPRFKETVLEGKEWEDSTRTPRDFSSAK
                     SLFDSPASKGTRSFSDDSSRTPRKRAAESGGHEKVQEQGSLAKEKKTEGKPAVSGTEK
                     NKREKEKKLQEGLLAPEEVAKPAKKKRRRKKPSASGDNKTTPVGTQQPRQQERGDGKQ
                     TESKYSEEASSGGRPSRKHASPELNQSNLSAEKTDGAPHSPENTTAPSARRSSSSRRR
                     RSGKGGGNRDTHEQNKSPEQKTEGSESKNASSGGAQQKRQKNKPHPRPKEESGESRKE
                     KGPE"
     gene            1053..1994
                     /gene="ftsY"
                     /locus_tag="P5201_02895"
     CDS             1053..1994
                     /gene="ftsY"
                     /locus_tag="P5201_02895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015556537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005047 - signal recognition particle
                     binding [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle-docking protein
                     FtsY"
                     /protein_id="HRV97511.1"
                     /translation="MSFFEKLKKSLQGVRKKWSSGLSGIFSGSAIDEEFWDRLEEVLI
                     SGDVGVTLSLRLIEDLKKEARARGAKTPEELFQFFADILGARLSAVPLMGQPVTPDKN
                     SLTIVILVGVNGSGKTTTAGKLASQWTKEGFRVVFAAADTFRAAAIDQLKVWGERSGV
                     RVIAQQQGSDAAAVAFDALSAAKSSRAHVLLVDTAGRLHSKHNLMEELGKIYRVLEKE
                     RGDAPLETLLVLDAVMGQNGLAQAQTFNKILPLSGVVLSKYDNTAKGGILLAIAEELK
                     MPVRYVGLGEGEDDLRPFDIHDFISALLEGDKNAGRE"
     gene            2777..3340
                     /locus_tag="P5201_02900"
     CDS             2777..3340
                     /locus_tag="P5201_02900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014163230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4416 family protein"
                     /protein_id="HRV97512.1"
                     /translation="MSVSTTDKPSRNTAYFYPVKLIVGVLYPDEALWTWTKNALTALW
                     GSPEDESAPYLFSSLTDYYADIAPVLFRRFLSFTTLRDAGELQEWKISACETEKRSGT
                     PRKVNVDPGYVNGARLVLASTKDHAHRIYIGGGIHAEVTLRYRRKRWTPFDYTFPDFA
                     DGRYNDFLSLVRKRWLDEMTARRLRND"
     gene            3333..4631
                     /gene="purB"
                     /locus_tag="P5201_02905"
                     /pseudo
     CDS             3333..4631
                     /gene="purB"
                     /locus_tag="P5201_02905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013048435.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004018 - N6-(1,2-dicarboxyethyl)AMP
                     AMP-lyase (fumarate-forming) activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate lyase"
     gene            4802..>4937
                     /locus_tag="P5201_02910"
     CDS             4802..>4937
                     /locus_tag="P5201_02910"
                     /EC_number="3.1.3.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008712297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042132 - fructose 1,6-bisphosphate
                     1-phosphatase activity [Evidence IEA];
                     GO_process: GO:0006071 - glycerol metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-bisphosphatase class II"
                     /protein_id="HRV97513.1"
                     /translation="MFAADRNLALELVRATEAAAMAAGRWMGRGDKNAVDGAAVNALR
                     Y"
BASE COUNT         1345 a         1229 c         1300 g         1063 t
ORIGIN      
        1 gctctgaatc cgacgaaaat atcgggcatc tgcggaaggc tgatgtgttg catgtcctat
       61 gagcacacat cgtacagcag cctctggaaa acgctcccga atccgggtgc aaaaatccgc
      121 actcccgccg gcaattatat cgttgaggga gttgagctct catccaactc cgtacgcgta
      181 cgccgtccgg acgggggaag ccttctcatc gtaattgaag attttccgcg tttcaaagag
      241 actgtcttag aagggaagga atgggaggac tctactcgta cgccccgtga tttctcatcg
      301 gcgaagtctc tttttgacag cccggcgtca aaaggcacac gttcattctc agacgactcg
      361 tcccgcactc ctcgcaagag agcagctgaa tccggcgggc atgagaaagt gcaggaacaa
      421 ggatctctcg caaaagagaa aaaaacggaa ggcaaacctg cggtttccgg aacagaaaag
      481 aacaaacgag aaaaagagaa aaagcttcag gaaggtcttc ttgcacctga agaggtagcc
      541 aaaccggcga aaaaaaagag acgaagaaag aaaccttcag cttccggcga caacaaaacc
      601 acaccggttg gaacccaaca gccgcgtcaa caggaacgcg gagacggaaa acaaacagag
      661 tcaaaatact cggaagaagc ctcttccggt ggtcgaccgt ccaggaaaca tgcgtcgcct
      721 gagcttaacc agagcaattt atcggcagag aaaaccgatg gagctcccca ttctcctgag
      781 aatactaccg ccccttccgc cagacggtct tcgagttcca gaagaagacg ttccggaaaa
      841 ggcggaggaa acagagacac acacgagcaa aataagtctc cggaacaaaa aactgaagga
      901 tccgaatcaa agaacgcttc ctcgggcgga gcgcaacaaa aacgacagaa aaacaaaccg
      961 cacccccgcc ctaaagaaga atctggagaa tcccgcaaag aaaaggggcc ggaataacgg
     1021 ctgcacgtcc cgaggaggag gcctgacagt acatgtcttt ctttgaaaag ctcaaaaaaa
     1081 gtctgcaggg agtacgcaaa aaatggagca gcggactctc cggaattttt tcgggcagcg
     1141 caatagatga ggagttctgg gatcgtctcg aagaagtcct tatctcagga gatgtaggcg
     1201 tcacgctctc actgcgtctg attgaggatt tgaaaaaaga ggcgcgtgca cgcggcgcca
     1261 agacgccgga agagcttttt caattcttcg cggacatctt aggagcccgc ctttctgctg
     1321 ttcctctgat gggacagccg gtaacgccgg acaaaaattc gttgaccata gtcattcttg
     1381 tcggcgtcaa tggaagcggg aaaaccacaa cagcaggtaa actcgcttcc caatggacga
     1441 aagaaggctt ccgagttgtc tttgcggctg ccgacacgtt tcgtgccgcc gcgattgatc
     1501 aattaaaagt atggggggag cgttcgggag tacgcgtcat cgcgcaacaa caagggagcg
     1561 atgcagccgc agtagccttt gacgcactct ctgccgcaaa atcgagccgt gcgcacgttc
     1621 ttcttgtcga tactgccgga cgtcttcact cgaaacataa ccttatggaa gaactcggaa
     1681 agatttacag agtgctcgaa aaggaacggg gcgacgctcc tctcgaaacg ctgctcgtac
     1741 tggatgccgt gatgggacag aacggactgg ctcaggcgca gacgttcaat aaaattctgc
     1801 ctctttcagg cgttgtcctc tccaagtacg acaatacagc gaaaggcggc attctccttg
     1861 ctatagcaga ggagttaaaa atgccggtac ggtatgtagg ccttggagaa ggggaagacg
     1921 accttcgtcc attcgatatc catgatttta tttcagcgct tctggaggga gataaaaatg
     1981 ccggacgaga atagagcaga agtgcgatac aacactacgc tcgaatctcc tctgtcgtcg
     2041 cttcttcgct ccctctatct gcacggagtt gagcattgcg cgcttgcgac atctcaaggt
     2101 ttttcactct actcgaagga tcagattgtc tctcttcttg aaagaggaaa cgccgataca
     2161 aaagtcagcg atgcaatcgc gttgctttca tcaaacggtt tcgcctgcct cgctgaacag
     2221 cgtctaatgg agcctttaac tccggtttta ctggttactg acaggggtgt tgagctcgtt
     2281 cccgcgggaa aaattccgca accttccccc aaacaagaat gcacagctcc ggacgaggga
     2341 acaacggact caggcctccc atgctggtgg acaataccgc ttcctctcgc tttcttaacg
     2401 gggaaaaaac cggttttaaa ccctgctgcc gacaaactcc tcgacgggtt tacactgcct
     2461 tctttcgccc ggaaaaaaaa gcaccccacg gagttcctca tcgagctcgg cagcaaatca
     2521 acgaaaaagc gaaatactat ctcaaggcat tttctgttca ctgaggtaaa ggactctttt
     2581 ttcctgatcc aggaggttac ggaagatctg cagagtgcgg aacaaatggc atggcttgct
     2641 tccatcggcc aagcatttct ttccgatctc aagtgcaaag gaataccggt ccggcagatt
     2701 cgttcgcccg aatatacagc cgaagaagag aacggaaggc gcctggcgtg catatgggag
     2761 gaaaaacttc ttggatatgt ctgtatcgac aacggacaaa ccgagtagga acaccgcgta
     2821 cttctatcct gtgaagttga tcgtcggggt tctctacccc gacgaagctc tttggacgtg
     2881 gacaaagaat gcgttgacag ccctgtgggg atctcccgaa gatgaaagcg ctccgtacct
     2941 tttttcttcg ctcactgatt attacgccga catagctccg gttttgtttc gacgttttct
     3001 ctcgttcaca actctccggg atgcaggaga gctgcaggaa tggaaaattt ccgcatgtga
     3061 gacggagaag aggagcggga cgccgagaaa agtgaacgtc gatcccgggt atgtgaacgg
     3121 agcgagactc gttcttgcct ctacgaaaga ccatgcgcac agaatctata tcggcggggg
     3181 gatccatgcg gaggtgacgt tgcggtacag gcgaaagcga tggacgccct tcgattacac
     3241 cttccccgat tttgcggacg gacgatacaa cgatttcctc tcgcttgtac ggaaacgctg
     3301 gctcgatgaa atgactgcga ggaggctgcg gaatgattga cagatataca accaaagaaa
     3361 tgaatgctat ttggagcgag gagaacaagt tcaggacatg gctcgaagtt gaactcgccg
     3421 tatgcaaagt gtggaacgaa cgcggcgtta tcccgtctga agcgtacgac gaaatatgcg
     3481 cgaaagcgga ttttcgtatt gaacggatca acgagatcga agaacaggta caacacgacg
     3541 tcattgcttt cgtctcagcc gttgcggaga ccgtcgggaa gaacggccgc tatattcatc
     3601 taggactgac gagcagcgat gtcatcgaca ctgcttcttc tctttcactc aaacgttctc
     3661 tgactgtagt ggacgaaaaa gtagtccagc tgatttcggc tgtaaacaaa cgcgcggaag
     3721 agttccgata cacgccgtgc gccggcagaa cacatgggat tcacggagag ccgactactt
     3781 tcggattgaa actgatgaac tggcaataac agcttcaaag agaccgccag cgaattgctc
     3841 ttgcggttga gcagataaat tacggaaaaa tctccggggc agtgggcaca tacgcacact
     3901 gcgatcccga ccttgaaaaa cgcgtttgcg acatactggg gcttcggcct gccctcgttt
     3961 ccacacaaat tctccagcgc gaccgccatg cgaacatttt gaacacgctc gctcttcttg
     4021 gaagcgccct cgaccgtttt gcaacggaga tccgtcatct tcagagaacc gaggttatgg
     4081 aagtcctgga acctttcggc gcacggcaga aagggtcgag cgccatgccg cataagcgga
     4141 acccgattct ctgtgaacgt atttccggta tgagcaggct gcttcgaggc tacgcgctca
     4201 caggaatgga aaacatcaat ctctggcacg aacgggatat cagtcactcg tccgcagaaa
     4261 gagttgtctg gcccgacgct tttcacacaa tttgctatat gctttcgaaa atgaagactg
     4321 tagttgaaaa tttacgagtc gatactgaaa aaatgaaaga gaaccttgaa ttaacgcgcg
     4381 gactggtgta cagtcaacgt atcctcctgg aactcgtcga gcgatttggg atgctccgcg
     4441 aggaggcgta cgccatagtt caggagaatg cgatgaaatg ctggcaagag aagattccgt
     4501 tttgcgatct tctgcttcgt gacgagaggg tttcttcttt ggtttccgaa acacagttgc
     4561 gttcgtactt tgcaccggaa tactatttcc gatgggttga cgaagtattc agccgatttt
     4621 ccgatgagta gaaatatccg caacaaaata aggtagagaa cgctaagggg tttcttaggg
     4681 gaaaattact agcaagcgac aaggtactcg gatactgtcc tttttgcagt tgtaccaaag
     4741 tgaacgattg gccttgtaaa atactaattt caaggaggat atctcaatgt gtgctgcaga
     4801 tatttttgct gctgacagga acctcgctct cgaattggta cgggcaaccg aagctgcggc
     4861 tatggctgcg ggaagatgga tggggcgcgg cgacaagaac gccgtcgacg gcgcggccgt
     4921 caatgcgctt cgataca
//
LOCUS       DAOTIL010000088         4935 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminobacteriaceae bacterium isolate AMR_MDS_3026
            NODE_20329_length_4935_cov_3.343852, whole genome shotgun sequence.
ACCESSION   DAOTIL010000088 DAOTIL010000000
VERSION     DAOTIL010000088.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868050
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminobacteriaceae bacterium (wastewater metagenome)
  ORGANISM  Aminobacteriaceae bacterium
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae.
REFERENCE   1  (bases 1 to 4935)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4935)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:19:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,224
            CDSs (total)                      :: 3,198
            Genes (coding)                    :: 3,184
            CDSs (with protein)               :: 3,184
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4935
                     /organism="Aminobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20329_length_4935_cov_3.343852"
                     /isolate="AMR_MDS_3026"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:3034609"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..859)
                     /locus_tag="P5201_02915"
     CDS             complement(<1..859)
                     /locus_tag="P5201_02915"
                     /inference="COORDINATES: protein motif:HMM:NF019121.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--D-alanine ligase"
                     /protein_id="HRV97514.1"
                     /translation="MQFPKNEPVTDRPLVVLLPAAMEQSPRTDVADALRGREFVSGIL
                     RAHGYVPAPLDITPEILQSPERIQHIVRSTKTRCVFNIFEGFGCDSGSEHQFRSLVEE
                     MSIPCTGNPSGVLQACLSKETCSRTLRKAGIPVPEGVSLHPGFDPELLASLRLPVFVK
                     PLMEDGSVGIDASSFVQEKSALLRTVLEKLSLFPRGVRAEEFIPGREYSVACIGNGPY
                     NVLGVSVIDYEQTEGTNFLDYDSKWDPASPLYSLVPQRAEGAFREKAAQLAATAGRTR
                     VLRSPRVMAE"
     gene            complement(864..1928)
                     /locus_tag="P5201_02920"
     CDS             complement(864..1928)
                     /locus_tag="P5201_02920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014163368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-grasp domain-containing protein"
                     /protein_id="HRV97515.1"
                     /translation="MNEEYGHEPDVPLQPVARVSVVYNLKKQAGSDEPDDKYEEYDPI
                     STVEAVAGAVGAYGFSVSLCEQDDDFAARLAEQKPQFVANIAEGRGEARGREAQVPCF
                     LESAGIPYWGSDAVSMAVALDKLLTSRTLLCDEIPVPLCACFKQEQDLSQLPALFTER
                     SRFVVKPRYEGSSKGIFSDSVAHTPDEAKEKIRRIWGRYAQPALLEEYLPGDEITVGI
                     VGNGRPSVVGMMRIVPVEQREEFLYSLEEKRDYLKRIRYEGPESIPASVRDQVGRFAL
                     KAFQSLELRDMARVDFRLDSDGTPRIIDINPLPGLSPEYSDLPILHRLSGGEYNDLVR
                     SILRSSFSRNGLAFPARGSR"
     gene            complement(2214..3005)
                     /locus_tag="P5201_02925"
     CDS             complement(2214..3005)
                     /locus_tag="P5201_02925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006302084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0140737 - encapsulin nanocompartment
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="family 1 encapsulin nanocompartment shell
                     protein"
                     /protein_id="HRV97516.1"
                     /translation="MDILRRSASLLTPEAWEELDEQAKKVLVANLSARKFVDVEGPKG
                     WTYSAHPTGRLEIDASNSGKSVQFGVNKVLPLVETRATFELDIWELDNITRGAKDPDL
                     YALEKAAKEIALFEEQAIYKGLATAGIEGLATAAKGSSVSLESEDPHGIVNGLSAAVF
                     ALREKAVEGPYAFVASPKLWKAIYSATPGYPTSKHVANLVDKVILSTQDESYVVSLRG
                     GDFELVLGQDLSLGFEERNGGKVRLFFAESFTFRVITPEAIVSLT"
     gene            complement(3047..3394)
                     /locus_tag="P5201_02930"
     CDS             complement(3047..3394)
                     /locus_tag="P5201_02930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006584419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferritin-like domain-containing protein"
                     /protein_id="HRV97517.1"
                     /translation="MQYTEPADKLDQQTVDQAQALKSLMEEVEAVNYYNQRAALTPNA
                     ELKALLAHNRDEEIEHAAMLIEWLRRNMDGWDEELKTYLFTSAPLTQVESGGGAGAES
                     GVSSKPLGIGSLK"
     gene            3687..4331
                     /locus_tag="P5201_02935"
     CDS             3687..4331
                     /locus_tag="P5201_02935"
                     /inference="COORDINATES: protein motif:HMM:NF017833.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exopolysaccharide biosynthesis protein"
                     /protein_id="HRV97518.1"
                     /translation="MDEQGKKTLLSEDIRELLSLKPGENVLFGELAAAVADRGFGILL
                     VLLSLPSALPVPAAGYSTPFGIVLFILGVQMLMGREKPWIPDWAGRKEIKRKTADKMI
                     SGAAKFFSFVEIFVRPRFEFVSGRSAEIACSLLLLLMSGLMILPIPLTNTLPAMVIFC
                     IGVALIERDGLAFIAALLFGAAATALYALAVWVIFYLGAQGIGEAKEIIKGWFF"
     gene            4411..>4935
                     /locus_tag="P5201_02940"
     CDS             4411..>4935
                     /locus_tag="P5201_02940"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="cache domain-containing protein"
                     /protein_id="HRV97519.1"
                     /translation="MSIRRKMLVLIAGFLLVTLVLTCGSYVLTGRSVRVQVEDTGRTM
                     VAEIASAMDEYIAKLLSLTGSLAVSLSYISEEGDPDYVELFGRYLEAAQTHGVQTVFM
                     GFESKEFADSTAWEPPEGYDPRTRSWYVETLKKNGVTVTSPYVDLITGELIVSVTAPV
                     RTQSGMLLGVAGLDV"
BASE COUNT          994 a         1318 c         1471 g         1152 t
ORIGIN      
        1 cttcagccat gactcgcggg gagcgtagca cccgggtgcg gcccgccgtc gccgcgagct
       61 gcgcggcctt ctcccggaac gcgccctccg cccgctgcgg gacaagcgag tagagaggcg
      121 aggcggggtc ccatttagaa tcgtaatcga gaaagttcgt tccttcggtc tgttcgtagt
      181 caatgaccga gacgccaagc acattgtacg ggccgttccc gatgcaggcg acggagtact
      241 cgcgtccggg aatgaactcc tccgcccgga ctccccgagg aaatagtgag agtttctcca
      301 ggaccgttcg aagcagcgcg ctcttttcct gcacgaacga ggaggcgtcg atgcctacgc
      361 ttccgtcctc catcaggggt ttgacaaaca ccggcaagcg aagcgatgcc aagagttcag
      421 gatcgaatcc cgggtggagc gagacccctt cggggacagg aattcccgcc ttcctgagcg
      481 tgcgcgagca cgtttctttc gagaggcagg cttgaagcac ccccgaagga ttgccggtgc
      541 aggggatgga catctcttcg acgagcgagc ggaactggtg ttcggaaccg ctatcgcagc
      601 cgaacccctc gaagatgttg aagacgcagc gcgtcttggt cgagcggaca atatgttgga
      661 tgcgctccgg agattggagg atctcgggcg tgatatcgag aggcgccggg acgtatccat
      721 gagcccgaag aatgccggag acgaattcac gcccccggag cgcatcggcg acgtccgtgc
      781 gcggagactg ttccatcgcg gccggaagca ggacgacgag aggcctgtcg gtcacaggct
      841 cgtttttcgg gaactgcatt cgatcaccgg cttcccctgg cggggaaggc caggccgttc
      901 ctggagaagg agcttctcag aatggaacgg acaagatcgt tgtactcccc gccgctgaga
      961 cggtggagga tcggaaggtc gctgtattcc ggggagagcc ccggaagcgg atttatgtcg
     1021 atgattcttg gagttccgtc gctgtccagc cggaagtcca cccgcgccat gtcccgcagc
     1081 tccagcgatt gaaacgcctt cagcgcgaag cggccgacct ggtcgcggac ggaagcgggg
     1141 atgctctcgg ggccttcgta gcggattcgc ttcaagtagt cgcgtttctc ttcgagagag
     1201 tagaggaact cttcccgctg ttcgactgga acgatgcgca tcatccccac cacggagggg
     1261 cggccgttgc cgacaatccc gaccgtgatc tcatctcccg gaagatattc ttccaacagc
     1321 gccggctgcg cataccggcc ccatattctg cggattttct cttttgcctc gtcgggcgta
     1381 tgcgcgacgg aatccgaaaa gattcccttg gaagaccctt cgtacctcgg cttcacgacg
     1441 aagcgggaac gctcggtgaa gagcgcgggg agttgcgata ggtcctgttc ctgtttgaaa
     1501 caggcgcaca gagggacggg gatctcgtcg cagagaagcg tcctgctggt gagcagcttg
     1561 tcgagcgcca ccgccatgga aacggcgtcc gatccccaat acgggatgcc cgcgctctcc
     1621 agaaaacagg gaacctgcgc ctcccgcccc cttgcctccc cacgtccttc ggcgatattg
     1681 gcgacgaact gaggtttctg ttcagcaaga cgcgctgcga aatcatcgtc ctgctcgcag
     1741 agggagactg agaatccgta tgccccaaca gcaccggcga ctgcctcgac agtcgaaatc
     1801 gggtcgtact cctcgtactt gtcgtccggc tcgtcggagc cggcttgttt tttgagatta
     1861 tagacgacgg aaactctggc gacaggttgc aacggaacgt cgggctcgtg cccgtactct
     1921 tcgttcatag attttcccct cctgaaggat acatgaatga tgacctgtga aaatgcggtc
     1981 aatgcggttt tccatttctt cgaatccgat gttcgggaac cgccatgtct ccctcctcca
     2041 agagtttgcc ccctccgtgc gaagagggtc tttcagtgcg gattatagag gtttttgggg
     2101 gaaatacaag aggggaattc cgttgcggga gagctttttc gcaggctgtg cgacgcaaaa
     2161 aaacaggggc tcgggagagc cccttttctg gagttcgcaa agcaagtttt ctgttaggta
     2221 agcgaaacga tggcttcggg ggttatgact ctgaatgtga atgactcggc gaagaaaagt
     2281 cgcacctttc cgccgttccg ctcctcgaag ccgagcgaaa gatcttgtcc gagaacgagt
     2341 tcaaagtcgc cgccgcggag cgagacaacg tacgactcgt cctgggtgga gaggatgacc
     2401 ttgtcgacga gattggcgac gtgttttgat gtcggatacc ccggtgttgc gctgtatatg
     2461 gctttccaga gtttcggaga tgcaacgaat gcgtatggac cttcgactgc cttctcccgg
     2521 agagcgaaca ctgccgctga aagaccgttg acgattccat gcgggtcctc gctctcaaga
     2581 gaaacggagc ttcctttcgc agcggtcgca agaccctcaa tacctgccgt agccaagccc
     2641 ttgtaaattg cctgttcctc gaaaagggcg atctccttcg cggccttttc aagcgcgtac
     2701 aggtcggggt ctttggctcc tctggtaatg ttgtcgagct cccaaatatc gagttcaaac
     2761 gttgctctgg tctccaccag aggaagcacc ttgttgacgc cgaactggac gctttttccg
     2821 gagtttgagg cgtcgatctc cagccttcct gtcgggtgtg cgctgtatgt ccaccccttg
     2881 gggccttcga cgtcgacgaa ctttcgagcc gaaaggttcg caacgagtac ttttttcgcc
     2941 tgttcatcaa gctcctccca agcctccggg gtcaggagcg atgcggatct tctcagaata
     3001 tccatgagtg tgtacctcct ttacatgttc gtctgctctg caggaactac ttgaggctcc
     3061 cgataccgag gggcttggaa gaaacgccgg attcggcgcc tgctcctccc ccggattcaa
     3121 cctgagtgag aggggccgag gtgaagagat atgttttcag ttcctcatcc catccgtcca
     3181 tatttctccg aagccattcg atgagcattg cggcgtgttc tatctcttcg tcgcggttat
     3241 gagcgaggag cgctttaagc tccgcgttcg gcgtgagtgc ggctcgctgg ttgtagtagt
     3301 ttaccgcttc tacttcctcc atgagacttt tgagcgcctg cgcttgatca actgtctgct
     3361 gatcaagctt atctgcgggt tcagtgtatt gcatggtatc tctcctttct ggaaatcacg
     3421 aatagatgat gccgaccgtg cggactcggc atttattcca acattatacc gtctgcagag
     3481 gaaaatcact cgtgtaaaaa agagacggtt ctaaataaat ttattacaag gtagttttta
     3541 ttttatgtga gtatatacca acttctgact ttcgtcaagt ttggtcaaat ggaagaatcg
     3601 cttcactgta tgatatcatg cggtgcgaag tgtgggggat gtccgctgaa aagcgtatca
     3661 tgacgagggg aaggtttgtg tacattatgg atgaacaggg gaagaaaacg ctgttatcgg
     3721 aagatattcg agagctgctc tcgttgaaac ctggagagaa cgtcttgttc ggagaacttg
     3781 cagcagctgt agctgatcgt ggattcggca tccttctggt gctcctttcg cttccgagcg
     3841 cgcttcccgt gccggctgca gggtacagca cgccgttcgg aatagtgctc ttcattctgg
     3901 gagtgcagat gctcatgggc agagagaagc cgtggatacc tgattgggcg gggcgaaagg
     3961 agatcaagcg gaagacggcg gacaagatga tatcgggtgc ggcgaagttt ttctcgttcg
     4021 tcgagatttt cgtacgcccc cgcttcgagt tcgtctcggg aaggagcgca gagattgcct
     4081 gttctttgct gctgctgctt atgagcgggc tcatgatcct tccgatacct ctaacgaata
     4141 cgctcccggc aatggtgatc ttctgcatcg gcgtagcgct catagagcgc gacgggctcg
     4201 cgttcatcgc ggcgctgctt ttcggcgcgg ctgctacggc gctgtatgct ctggcagtct
     4261 gggtaatttt ttacctcggg gcccagggaa taggcgaggc gaaggagata atcaagggct
     4321 ggtttttctg atcccgaact tttctgtaca cgtcatgatt cgccaatgat ttttcagggt
     4381 ttccccggaa acgagagggg tgatactgct gtgtctattc gacggaagat gctggtgttg
     4441 atcgccgggt ttcttcttgt aactttggta ttgacttgcg gcagctacgt cctcaccgga
     4501 aggagcgtgc gcgtccaggt cgaagatacc gggcgaacta tggttgcgga gatcgcctcc
     4561 gctatggatg aatatatagc gaaacttctc tcccttactg gctcgctggc tgtttctctc
     4621 tcatacatca gcgaggaggg cgatcctgac tatgtagaac ttttcggccg ctatctggag
     4681 gctgcgcaga cgcacggagt gcaaacggtc ttcatgggct ttgaatcgaa ggagttcgcc
     4741 gattctaccg catgggagcc gccggaaggg tacgaccccc ggacgcgctc gtggtatgtg
     4801 gagacgctga agaaaaacgg ggtgacggtc acctctccat atgtcgacct gataaccgga
     4861 gagctcatcg tgagcgtcac ggcgcctgtg agaacccagt cggggatgtt gctcggtgtg
     4921 gcggggctgg atgtg
//
LOCUS       DAOTIL010000089         4898 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminobacteriaceae bacterium isolate AMR_MDS_3026
            NODE_20520_length_4898_cov_3.207722, whole genome shotgun sequence.
ACCESSION   DAOTIL010000089 DAOTIL010000000
VERSION     DAOTIL010000089.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868050
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminobacteriaceae bacterium (wastewater metagenome)
  ORGANISM  Aminobacteriaceae bacterium
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae.
REFERENCE   1  (bases 1 to 4898)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4898)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:19:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,224
            CDSs (total)                      :: 3,198
            Genes (coding)                    :: 3,184
            CDSs (with protein)               :: 3,184
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4898
                     /organism="Aminobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20520_length_4898_cov_3.207722"
                     /isolate="AMR_MDS_3026"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:3034609"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..511)
                     /locus_tag="P5201_02945"
     CDS             complement(<1..511)
                     /locus_tag="P5201_02945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013048291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ClpX C4-type zinc finger protein"
                     /protein_id="HRV97520.1"
                     /translation="MFRFDDRGRGSKSNGGKNLHNGRCSFCGKGQDEVQKLIAGPGVY
                     ICDECIHLCNMILVEEAGETAAKAKTAEPVFPLSEVPKPREIKEYLDQYVIGQDLAKK
                     AVSVAVYNHFKRISNNMAEESDVELQKSNVLLMGPTGCGKTLIAQSLAKKLNVPFAMA
                     DATTLTEAGY"
     gene            complement(736..1314)
                     /gene="clpP"
                     /locus_tag="P5201_02950"
     CDS             complement(736..1314)
                     /gene="clpP"
                     /locus_tag="P5201_02950"
                     /EC_number="3.4.21.92"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014163219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009368 - endopeptidase Clp complex
                     [Evidence IEA];
                     GO_function: GO:0004176 - ATP-dependent peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp endopeptidase proteolytic
                     subunit ClpP"
                     /protein_id="HRV97521.1"
                     /translation="MLVPYVVEQTGRGERTYDIYSRLLKDRIIFLGDSVDEHTGNIVV
                     AQLLFLESEDPEKDIHLYINSPGGYVSSGLAIYDTMQYIKCPVSTICLGHAASMAAVL
                     LAGGATGKRFVLPNARIMIHQPLGGVQGQATEVEIHAREILKLRERLNDILVKHTGQS
                     KKKVRADTERDFYMSPEEAVEYGIVDKVIHSR"
     gene            complement(1347..2690)
                     /gene="tig"
                     /locus_tag="P5201_02955"
     CDS             complement(1347..2690)
                     /gene="tig"
                     /locus_tag="P5201_02955"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: protein motif:HMM:TIGR00115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005854 - nascent polypeptide-associated
                     complex [Evidence IEA];
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0051083 - 'de novo' cotranslational protein
                     folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trigger factor"
                     /protein_id="HRV97522.1"
                     /translation="MRSEILSQENNVVSIKLEFEASEFAQGVEKAIKDLSTKVNIAGF
                     RKGHVPRKVMEMRFGRQAIYAEAIEAMLPDAIEQIVKDYDLDLIDEPHVSIEKMEEGS
                     PVDVRLTFEVSPEVVLPEVSAVTVHRPVASVADEIVSGTIEDIRAQNAEHSPVENVPV
                     AENHVVDIEYHTVVLLDEGKQERHGPDSTTLDLGQPSVRTEIRDALLGAKKGESRTAD
                     VQVEENYQDPKVAGRTVRYELTVNEIKEKVLPEMTPDFFKKILRSDCETEELFREEIK
                     KRILEKIASEIQGKAEFDALQGISDLSQLTVPETLISRQMTAMKKEDEENIKKSRNMS
                     LEEMLSEASTTMEEYEKNIREQAETIVRRSLVLDKVADEMGITVEKEDFEAEMQTLAS
                     SYKIDAQRLVEGLFKDEKRLMEIANRIKYKKTMKAIMNTVQIDETEPAAEEKPAE"
     gene            complement(2918..3121)
                     /locus_tag="P5201_02960"
     CDS             complement(2918..3121)
                     /locus_tag="P5201_02960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006301144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold shock domain-containing protein"
                     /protein_id="HRV97523.1"
                     /translation="MTTQGTVKWFNATKGYGFVTCDDGKDVFVHYSAIQGDGFKTLDE
                     GQRISFRITQGSKGDQAADVEKI"
     gene            complement(3346..4716)
                     /locus_tag="P5201_02965"
     CDS             complement(3346..4716)
                     /locus_tag="P5201_02965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006301642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD binding domain-containing protein"
                     /protein_id="HRV97524.1"
                     /translation="MKFELTVNGKVCSVDTPPMRRLLDILRQDLGLPGAKEGCGEGEC
                     GACAVLLDGRLVNSCLIPAFQLPGRSVETIEALSVDGTPDTLQQAFLDAGAVQCGFCT
                     PGLVMASRALLSKNAHPTRKEIREGIAGNLCRCTGYEAVVHAVEQAAEKGYAPSLVPA
                     KRLDASVLLEKEEEGKVFLPRTLEEAFEALSVHGDELAVVAGGTDLMVDMEKKGLPAP
                     EKVLDITGIEGMRRIGVSGDWLEIGAGATFAEIAVHENVLSVAPALAHCASQVGAVAI
                     QNRATIGGNIATASAAADSPPVLMALGAVLVLASGKGSREMPVEEFFSEYRRTARRAD
                     ELLVTVRIPLSVRGTRQMFRKVGLRQALAISRVSVACVAALESGVVTSCRIAAGSMSP
                     TPLLLKDVCSAVTGRALSRELAEEAGRVADSLVSPRTAPEYRKETTGNLVRRFFTDIL
                     KENATV"
BASE COUNT         1064 a         1442 c         1181 g         1211 t
ORIGIN      
        1 cataaccggc ctctgtgagc gtcgtcgcat cggccatagc gaagggaacg ttgagcttct
       61 tcgccaagga ctgcgcgatc agcgtcttgc cgcaccctgt aggccccatc agcaggacgt
      121 tgctcttttg cagctcgaca tcgctctctt ccgccatgtt gttgctgatc cgtttaaaat
      181 ggttgtatac tgcgaccgaa acggctttct tggccagatc ttgacctatg acgtactgat
      241 ccagatactc ttttatctct cgtggttttg gaacctccga aagcggaaag acgggctctg
      301 cggttttcgc tttcgcagca gtctccccgg cctcctcgac caggatcata ttgcacagat
      361 ggatacactc gtcgcaaata tacacgccgg ggcctgctat gagcttctgc acctcgtcct
      421 gcccttttcc gcagaacgaa cagcgtccgt tatggagatt tttgccgccg ttactcttgg
      481 aacctcttcc cctgtcatcg aacctgaaca ttcataaacc tccctgcaac ctctctaccc
      541 gggatacagc aacaagggag cgcctgaact cccatcccct gtacaggaat gcaagccgac
      601 aaactcccgg gcaagaaggg tattttccct ctatgccgac ggtgcgtttc cataaacgcg
      661 ccggagcgtt gcctgcttgt ctcgggctcc cttgaagtcg aatcaaaaca gttgtttccg
      721 gaactgaagc gcgctctatc tcgaatggat gactttatcg acgatcccgt actcaacagc
      781 ttcttccgga gacatgtaga aatctctttc cgtatcggcc cgtactttct tcttcgactg
      841 tcccgtgtgc ttcacaagta tatcattgag cctttcacgc agcttcagta tttctcgtgc
      901 gtgaatttcc acttccgtcg cctgcccctg cacgcctccg agaggctggt ggatcatgat
      961 gcgtgcgttc ggcaaaacaa agcgttttcc cgtcgctcca cctgcaagaa gaaccgcggc
     1021 catactcgcg gcatgtccaa ggcagatcgt ggaaacaggg catttaatat actgcatcgt
     1081 atcgtaaata gcgagtcccg aggagacata ccctcccggg ctgttgatat agagatgaat
     1141 gtccttctcg ggatcttcac tctcaagaaa aagcagctgt gcaacaacga tattgcctgt
     1201 atgttcatcg accgaatccc caaggaagat aattctgtcc ttgagaagac ggctgtatat
     1261 atcatatgtc cgttcgcctc gcccggtctg ttctacaaca tatggaacca acaaagggaa
     1321 cacctcctgt gatcgtagcg ttgttcctat tcagcgggct tctcttccgc cgcaggctcc
     1381 gtttcgtcga tctgtacagt attcatgata gccttcatcg tctttttgta ttttatcctg
     1441 ttcgctatct ccatcagcct tttttcatct ttgaagagcc cttcaacaag gcgttgcgca
     1501 tcgattttgt acgatgaggc gagcgtctgc atctccgctt caaagtcttc tttttccacg
     1561 gtgattccca tttcatcggc gaccttgtcg agcaccagcg atctgcggac gatggtctca
     1621 gcctgttcgc ggatattttt ctcgtactct tccatcgtcg tggaagcctc ggagagcatc
     1681 tcttcaagcg acatattccg gctcttttta atgttctctt cgtcctcttt cttcatcgcc
     1741 gtcatctggc gggaaatcag agtctccgga accgtaagct gagaaagatc ggagatgccc
     1801 tgcaacgcat caaactcggc tttcccctga atctccgagg ctattttctc caggatgcgc
     1861 ttctttatct cctcgcggaa aagttcttct gtctcgcagt cgctcctgag tatcttcttg
     1921 aagaagtcag gcgtcatctc gggaaggact ttctccttaa tctcattgac ggtcagttca
     1981 taccgtacgg tacgtcctgc gactttggga tcctgataat tctcctccac ctggacatcc
     2041 gcagttctgg actcgccttt cttcgcgccg agcagcgcat ctcggatctc ggtgcgtaca
     2101 gagggctgcc ccagatcgag cgttgttgaa tccggcccgt ggcgctcctg tttcccttca
     2161 tcaagcagca caacggtatg atactcgata tccacaacat gattttcagc aaccggaacg
     2221 ttctccacag gagagtgctc cgcgttctgc gctcgtatgt cctctattgt gccggagacg
     2281 atttcgtcgg caacagaagc gacgggacga tgtacggtga cagccgaaac ctccgggaga
     2341 actacctccg gagaaacttc gaaggtcagg cggacgtcca caggactccc ctcttccatc
     2401 ttttcaatcg atacatgagg ttcgtcaatg agatcgagat cataatcttt cacgatctgc
     2461 tctattgcat cgggaagcat cgcttcgatc gcctcggcat agatcgcctg cctgccgaaa
     2521 cgcatctcca taactttgcg cggcacgtgc cctttacgga aaccagctat gttcaccttt
     2581 gtcgacaggt cttttatcgc cttttctact ccttgggcga actccgatgc ttcgaactca
     2641 agtttgatgg agacgacatt attctcctgg gaaagaatct cggatctcat acaatcgcaa
     2701 cccctttcag tgcgattcgc actggtgtaa ttttttctct ataatgtatc tcattcctac
     2761 aggaagagaa aatatgcgga aggtattgta cacggaacac tgagagaatg caaagaaatg
     2821 caggctctct ttctggcaaa gcaaagtagt cgccaaaaat aaaagaaaaa ggcagcggaa
     2881 aaccgctgcc ttggaaatgg cgatatgtcg aacgaagtta tatcttctct acgtcggcag
     2941 cctgatctcc cttggaaccc tgagtgatac ggaaggaaat ccgctggccc tcgtcgagag
     3001 tcttgaatcc atctccctgg atagcgctat aatgaacgaa aacgtccttg ccatcgtcgc
     3061 aggttacgaa tccataaccc ttcgtcgcgt tgaaccattt tactgtgcct tgagttgtca
     3121 aagttgttcc tcctaagctt gattgcttgt gctttttgct gacggcgcct gtcaacgcat
     3181 acatcataat aagaaatgaa gagcatgtca agtctttctt gctttaaatt cgcactttct
     3241 tttcgttaca tcctgcgttt cttccggaat ccgcacgaga aaacaaaaaa atgccgctct
     3301 gccggcttct tttcgctctg ccgtttgcgg cctgctccgg atcgatcaga ccgtcgcgtt
     3361 ctccttgaga atatcggtga aaaaacgtcg tactaaattt ccggtagttt ccttcctgta
     3421 ttccggcgcg gtacggggag acaccagcga atcggccact cttccggcct cttccgccag
     3481 ctctctgctc agagctctgc ctgtgactgc cgagcagacg tccttcagga gcaggggcgt
     3541 cggcgacata ctgcccgcag ctattcggca cgaagtcaca acgccgctct ccagcgctgc
     3601 gacgcaggca acggaaaccc gtgaaatggc aagcgcctgc cggagaccca ccttccggaa
     3661 catttgacgg gttcctctca ctgaaagagg gatacgcacc gtgaccagca gttcgtctgc
     3721 acgccgggca gtgcgtctgt attccgagaa aaattcctct accggcattt cacgcgatcc
     3781 tttcccgctt gccaaaacaa ggacggcgcc gagggccatt aagacgggtg gagagtccgc
     3841 tgccgccgaa gcggtagcga tgttgcctcc gatggttgcc cgattctgaa ttgcgacggc
     3901 gcccacctgt gaagcgcagt gcgccagagc cggagctacg gagaggacat tctcatgcac
     3961 tgcgatttca gcgaaggtcg cgcccgcgcc tatctccagc caatcaccgg aaacgcctat
     4021 gcgacgcatc ccctcaattc ccgtgatatc gagaactttt tcaggcgcgg gaagcccttt
     4081 tttctccata tccaccatca ggtcggtgcc gcccgcgacc acggcgagct catctccgtg
     4141 aacggacagc gcctcaaagg cctcttcaag agttcgaggg aggaacactt ttccctcttc
     4201 ttctttttcg agaagcacgg aggcatccag acgcttcgcc ggaacaagac tgggtgcgta
     4261 gcctttttcc gcagcctgct ccacagcgtg caccaccgct tcatacccgg tacagcggca
     4321 gagatttccg gcgatgcctt cgcgaatttc tttacgtgta gggtgtgcgt tcttcgacag
     4381 aagcgctctc gatgccatga ccaaaccagg agtgcaaaaa ccgcactgca ccgcaccggc
     4441 atcaagaaag gcctgctgca gtgtatcggg cgtaccatcc acggagagcg cttctatagt
     4501 ctccacactt ctgcccggca gctggaaagc ggggataagg caagagttta caagacggcc
     4561 gtcgagaagc acggcgcatg ctccgcattc cccctcgccg cacccctcct tggcgcccgg
     4621 caggcccaga tcctggcgga gaatatcaag caaacggcgc attggaggcg tgtccaccga
     4681 acagacctta ccgtttacag tgagttcaaa cttcatagag ttccctcctt acttccggcg
     4741 ttgccgcaac agagcatgca aacggtcgct ggtcaacgga agctccgttg cgaagacgcc
     4801 aagagcatcc tcgacggcgg cggccaaggc aggcgcgccg ccgtccatcg ggagctcgcc
     4861 caatcctttg gcgccccagg gaccgaaagg agacggcg
//
LOCUS       DAOTIL010000090         4881 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminobacteriaceae bacterium isolate AMR_MDS_3026
            NODE_20622_length_4881_cov_3.912350, whole genome shotgun sequence.
ACCESSION   DAOTIL010000090 DAOTIL010000000
VERSION     DAOTIL010000090.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868050
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminobacteriaceae bacterium (wastewater metagenome)
  ORGANISM  Aminobacteriaceae bacterium
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae.
REFERENCE   1  (bases 1 to 4881)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4881)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:19:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,224
            CDSs (total)                      :: 3,198
            Genes (coding)                    :: 3,184
            CDSs (with protein)               :: 3,184
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4881
                     /organism="Aminobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20622_length_4881_cov_3.912350"
                     /isolate="AMR_MDS_3026"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:3034609"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..477)
                     /locus_tag="P5201_02970"
     CDS             complement(<1..477)
                     /locus_tag="P5201_02970"
                     /inference="COORDINATES: protein motif:HMM:NF013126.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 3 N-terminal
                     domain-containing protein"
                     /protein_id="HRV97525.1"
                     /translation="MITSKKAVCTLLLGLFFLIPGFMRISEANTRDLSSMTLEEKVGQ
                     IMLVFFEGGAMSENLQKFISEIKVGGVILYSSRNNIESVEQVANLNESIQRRALASSS
                     RPLFIAIDQEGGLIARIREGVTPFPGNMALGAAGDPSLAGRVASVMGEELASLGINM"
     gene            complement(474..1499)
                     /locus_tag="P5201_02975"
     CDS             complement(474..1499)
                     /locus_tag="P5201_02975"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HRV97526.1"
                     /translation="MLLLLLYFAFSMFYVFSIKNSLFSQLPPLQMAAQGIIICIVSME
                     AGAHPILPWVGVLAAVSGFFILLHSYSQYGRGTHLPPPVAHAESPSSDTASEPWMRSP
                     FPYVVLDNEGTIIDANDAFASFAENGSVQGENVTTFFVPGEIEAVVGGKKHAIFQKAK
                     DGQFHFLLVDNPPAQKNSKTAEAKSELELFDPKYGLYSRKYAQLRIPEELGRANRYRR
                     WLCGILLKVNYTYLPGRGRQPALEETFLAAYVLYVKESIRESDMGFSMQDGEFLLLLP
                     ETPQQGAKDTALKLIVVPESLDEMKKDYPFSVDIEYGFLYYSGNYPLSYDQFIQKLYA
                     SLGGSAE"
     gene            complement(1672..3198)
                     /locus_tag="P5201_02980"
     CDS             complement(1672..3198)
                     /locus_tag="P5201_02980"
                     /inference="COORDINATES: protein motif:HMM:TIGR02231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4139 domain-containing protein"
                     /protein_id="HRV97527.1"
                     /translation="MKMGVFNVSLLLFFLLCVLSVPSEAVSEYSILRTDMYPAGARLV
                     FSIPASEKVHFQLPGAFDSASVRPLPSEGVEVLSFEAVDVSRSEWIPPVLEKLFTSIR
                     EKERLQATLGGKISAIENSLALLSGPLPKELKGADVAEYIESVRAVREKMELERASLA
                     DTLDELQKELAVLQADFKSKMPPDSQKAVEITAVLSGSGVFLVEAFTRYAEWVPFYRM
                     NLSVSSGGISAGLFARARQKTGLLVEGTVHFHTSMPSVSVAPPELRPLVADLAPKHKD
                     VRRFAMDEALPMMAPMVQAPAPAEPAPRPVIQTLTDLSTQAEGLLTGDYTPADFNLGS
                     FSLKSETSIISVPSLSEQAWLVAESKGLPFPILPASAELSVDGSPSGKTTLDEHGSGS
                     DILIAFGRTPRVKASKEKLVSKEGSTWTGRGRTEDGYTIEVTNGMPHPVAVTLKDRIP
                     ISSRENISVEVLRIEPKPDEQSEQNLLTWKLSLAPGEKRTINAVFRLSYPSDETVIFR
                     "
     gene            3373..4464
                     /gene="ychF"
                     /locus_tag="P5201_02985"
     CDS             3373..4464
                     /gene="ychF"
                     /locus_tag="P5201_02985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012869284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="redox-regulated ATPase YchF"
                     /protein_id="HRV97528.1"
                     /translation="MLKCGIIGLPLSGKSTVFNVVTRAGAEVKPYASGKTDPNRAMVN
                     VPDDRFDHLARLFEPKKVTPATVEFVDLAGLSRNASKGEGVGNAFLSFVSESDALVHV
                     VRLFENPDVPHPENSIDPVRDWNIVEMELIFRDLGVIENRISRLQAKKKNIPSETEEK
                     ALLERCQACLLEERPLRELGLSPDELRLLSGFAFVTLKPELIVANLDETQTDDSLPLL
                     NALRELAKTKGIRLLRIFGKMEMELTELPEEEQKEFMGELGIEESGRNRLISEAYALL
                     GLLSFFTTGKDEVRAWTLVKGANAVDAAGAIHSDLARGFIRAQVVSYEDFISHEQSLA
                     KCREKGVLRLEGKDYVVNDGDMIEIRFNV"
     gene            4513..>4881
                     /locus_tag="P5201_02990"
     CDS             4513..>4881
                     /locus_tag="P5201_02990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006583887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRV97529.1"
                     /translation="MAMPMQVSLDEILSMLLARVDSLAINDENSKSKFNIVARVLYKK
                     GILTDADIVESIKEEHRMLKELGAIAEEPSDDIVETIADGILQWIKGDVESLKKGMEE
                     YERKVQEAMAKQQAQKPKIDV"
BASE COUNT         1285 a         1215 c         1274 g         1107 t
ORIGIN      
        1 catgttgata ccgaggctcg caagctcttc ccccattact gaagcgacac gaccggccag
       61 agacggatcc cctgccgctc caagagccat attacccggg aaaggggtaa caccctcgcg
      121 gatacgggca atcagaccgc cctcctgatc aatagctatg aagagcggac gagaacttga
      181 agcgagagct ctccgttgaa ttgactcgtt caggttcgca acctgttcga cggactcgat
      241 gttgtttcgg ctggagtaca ggatgactcc tccgaccttg atctccgaga tgaacttttg
      301 aaggttctcg gacattgctc ctccctcgaa aaaaaccagc ataatctgtc caactttctc
      361 ctcaagcgtc atagaagaaa gatctcgcgt attcgcttcc gagatgcgca taaatccggg
      421 gatcagaaaa aaaaggccga ggagcaatgt gcagacagct tttttcgaag taatcattcg
      481 gcgctccctc ctaaagaagc ataaagcttt tggataaact gatcatacga aagaggatag
      541 ttcccgctgt aatacaaaaa tccatactct atatcgaccg agaaaggata gtccttcttc
      601 atctcatcaa gcgattcggg aactactatt agtttgagtg ccgtgtcttt tgcgccttgc
      661 tgtggagttt cagggagaag gagcagaaac tccccgtcct gcatggaaaa gcccatatcg
      721 ctctccctga tggactcctt gacgtagaga acatacgcgg caaggaaagt ttcctccagg
      781 gcaggctgcc ggcctcttcc gggaaggtag gtgtagttca ccttgagtaa aatcccgcaa
      841 agccagcgtc tgtacctgtt tgctctgccc agttcctccg ggatacgcaa ctgggcgtat
      901 ttcctggagt agagtccgta tttcgggtca aagagttcga gctcgctctt tgcttcagcg
      961 gtctttgaat ttttttgcgc aggagggttg tcaacaagta aaaaatggaa ctgaccgtcc
     1021 ttcgccttct gaaaaattgc atgcttcttt cccccgacaa cagcttcaat ctcaccggga
     1081 acaaaaaagg ttgtcacatt ctccccttga acagaaccgt tttcggcaaa agacgcgaag
     1141 gcgtcgtttg catcgatgat tgtcccctcg ttatccaaaa caacgtatgg gaaaggagac
     1201 ctcatccaag gttcggatgc tgtatcagag gaaggggatt cagcgtgcgc gaccggcgga
     1261 ggaagatgtg ttccccttcc gtactgcgag tacgagtgca acaatataaa aaaaccagaa
     1321 acagcagcga gtacgcctac ccaggggaga atcggatgag cgcccgcctc catggaaacg
     1381 atgcatatga tgattccctg ggccgccatc tgaagcggtg gtaattggga aaacaagctg
     1441 ttctttattg aaaaaacata gaacatgctg aacgcaaaat acaaaagaag taaaagcacc
     1501 cacaaagggg cccccgccga tactttgttg ctctcatttc cgaaagcgac gagaaggaga
     1561 gagagaggca gcgaaagcag cggaggtatg gtgagccata aaatcatggc tggattctcc
     1621 taattatatg tttgcttctc ctggacgccg agtaccgcag aatgtagtat ttcaacgaaa
     1681 aatcaccgtc tcatctgatg ggtagctcaa gcggaaaacc gcatttatcg tacgtttttc
     1741 accgggagcg agagacagct tccatgtcag gagattttgt tctgactgtt cgtccggctt
     1801 gggttcaatg cgcaatactt cgacagaaat attttcccgc gatgaaattg gaatacggtc
     1861 tttcagcgtg acagcgacag gatggggcat gccgtttgtg acttcaatcg tatacccgtc
     1921 ctcggtgcgt cctcttcccg tccatgtgct tccttccttt gagacgagtt tctccttgga
     1981 agctttgact cgaggcgttc tgccgaaggc aataagaata tcggaaccgg agccgtgctc
     2041 atcaagcgtt gttttcccgg acggacttcc gtcgacggag agctcggcgg aggctggtag
     2101 tatagggaag ggaagccctt tggactctgc tacgagccat gcctgctcag agagagacgg
     2161 gacggaaata atagaagtct ctgacttcag cgaaaagctg cccaggttaa aatctgccgg
     2221 agtataatcc cccgtcagca gtccctcagc ctgagttgaa aggtcggtaa gcgtctggat
     2281 aacggggcga ggcgccggtt ctgcgggggc gggagcctgc accattgggg ccatcatcgg
     2341 gagagcttcg tccatggcga aacgccgaac atctttgtgt ttcggagcca gatccgcgac
     2401 gagcggacgc agttccggag gcgccacgga aacagaaggc atcgacgtat ggaagtgtac
     2461 ggtcccttcg acaagaagcc ccgtcttttg ccgcgctcgc gcaaaaagtc cggccgaaat
     2521 accgccgctg cttacggaga ggttcattct ataaaagggg acccactccg cgtagcgtgt
     2581 gaatgcttcc acgagaaaaa cgccgctgcc ggaaagaact gccgtaattt ctaccgcttt
     2641 ttgagagtcc gggggcattt tcgatttgaa atccgcttga agaacggcga gttccttttg
     2701 gagttcatct aaagtgtccg ctagagaagc gcgttccaat tccatttttt cccggactgc
     2761 ccggactgat tcaatgtact ccgctacatc ggcgcctttt aattcctttg gaagaggtcc
     2821 ggaaaggagc gccagcgagt tctcgatggc cgaaatcttg ccccccagag tcgcttgtag
     2881 tcgttctttt tcacgaatag acgtaaaaag tttctccagt acgggaggaa tccattccga
     2941 acgcgatacg tcaacggctt cgaaagagag aacttccacc ccttcagagg ggagcgggcg
     3001 aaccgaagcg ctgtcgaaag ctccgggcag ctggaaatgc accttttccg aagccggaat
     3061 agaaaagacc aaccgtgcgc cggcagggta catatctgta cggagaatcg aatactcaga
     3121 gacagcctcg gagggaacgc ttaggacgca caacagaaaa aaaagcaaaa gcgatacatt
     3181 gaagacgccc atcttcatct cgaaaaacct ccttgatcaa ggtataaaag cttgcaatca
     3241 atgataacag aaatttgaaa aaccggattg actctttttt aaagtacacg cgattcttcc
     3301 ctttcggcgt ggaaaaggta taatgcatca cgggactgaa aaaaactgtc tcgcaacaaa
     3361 ggaggaagaa aaatgctcaa atgtggtatt atcggccttc cgctgagcgg aaagagcacg
     3421 gttttcaacg tagtcacacg tgccggcgcc gaagttaagc cttatgcaag cgggaagacg
     3481 gatcccaaca gagcaatggt caacgtcccc gatgatcgtt tcgaccacct ggcacgcttg
     3541 ttcgagccca aaaaagtcac tccggcgacc gtggaattcg tcgatcttgc aggtctttcg
     3601 cgcaacgcca gcaaaggaga aggggtagga aatgcttttc tctcttttgt ctccgaatct
     3661 gacgcattgg tccacgttgt ccgccttttt gagaatcccg acgtcccgca tccggaaaat
     3721 tccatcgatc ctgtaagaga ctggaatatc gtcgagatgg aattaatctt ccgcgacctt
     3781 ggcgttattg aaaacaggat ttcccgcttg caggcaaaga aaaaaaacat tccttccgaa
     3841 acggaggaaa aagcgctgct tgaacgctgc caagcctgcc tccttgagga gcgccctttg
     3901 agagaactcg ggctttcgcc cgatgaattg cgtcttctca gcggttttgc ttttgtaacg
     3961 ctgaagccgg aactcatcgt cgcgaatctc gacgaaaccc agaccgatga ttcgcttcct
     4021 ctactcaacg cactccgcga gctggcgaag acaaaaggca tccgtttact caggatcttc
     4081 gggaaaatgg agatggagct tactgaactt cccgaggaag agcagaagga gtttatgggc
     4141 gaactcggca tagaagaatc gggccggaac cgccttatct ccgaagccta cgcccttctt
     4201 gggcttctct cgtttttcac gacgggaaag gatgaggtca gggcatggac gcttgtaaaa
     4261 ggcgcaaatg cggttgacgc tgcaggagcg atccattcgg atcttgcacg tggttttatt
     4321 cgggcgcagg tcgtctccta cgaagatttt atttctcacg agcaatcttt agcaaaatgc
     4381 cgggaaaagg gcgtgcttcg ccttgagggc aaagactacg tcgtaaatga cggcgatatg
     4441 atagaaatcc gcttcaatgt ctaatgtgat acaatcagta ctctaatttt cctagaaagg
     4501 ggcgcttttc caatggccat gccaatgcag gtaagtctcg atgaaattct ctcaatgctc
     4561 ctcgccaggg tggatagcct tgcgatcaac gatgagaatt caaagagcaa gttcaatatc
     4621 gtcgcacgcg ttttgtataa aaaggggatt ctcacggacg cggatatagt ggagtcgatc
     4681 aaagaagagc accgcatgct gaaggagctt ggcgccatag ccgaagagcc ttccgacgac
     4741 atcgtggaga ccatcgccga cggaattctc cagtggatca aaggtgatgt tgaatctttg
     4801 aagaagggga tggaggagta cgagcgcaag gtccaggagg caatggccaa acagcaggct
     4861 cagaaaccga agatagacgt c
//
LOCUS       DAOTIL010000091         4814 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminobacteriaceae bacterium isolate AMR_MDS_3026
            NODE_20990_length_4814_cov_3.138264, whole genome shotgun sequence.
ACCESSION   DAOTIL010000091 DAOTIL010000000
VERSION     DAOTIL010000091.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868050
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminobacteriaceae bacterium (wastewater metagenome)
  ORGANISM  Aminobacteriaceae bacterium
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae.
REFERENCE   1  (bases 1 to 4814)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4814)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:19:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,224
            CDSs (total)                      :: 3,198
            Genes (coding)                    :: 3,184
            CDSs (with protein)               :: 3,184
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4814
                     /organism="Aminobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20990_length_4814_cov_3.138264"
                     /isolate="AMR_MDS_3026"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:3034609"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..715)
                     /locus_tag="P5201_02995"
     CDS             complement(<1..715)
                     /locus_tag="P5201_02995"
                     /inference="COORDINATES: protein motif:HMM:NF013703.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015297 - antiporter activity [Evidence
                     IEA];
                     GO_function: GO:0042910 - xenobiotic transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HRV97530.1"
                     /translation="MNTDKHLLGTENVGKLLLRLSLPSVIGMLVMASYNIADSIFIGR
                     GVGPLGLASVMICFPIQFLGGALAIMAGVGGASVVSRSLGAGDTDRAQQAFGATAAFS
                     LSVGLLVSSLSLFFLPSLLRLFGATEGILPYAEDYLSVILFGVPFQMFAMAGNHMARS
                     EGRARIAMTTMLISALLNIILDPLFIFGFGWGIKGAAVATVISQLVMFVWIFGYFYSG
                     RSSLRFIRKNLFPKLALIRE"
     gene            complement(737..1225)
                     /locus_tag="P5201_03000"
     CDS             complement(737..1225)
                     /locus_tag="P5201_03000"
                     /inference="COORDINATES: protein motif:HMM:NF015908.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheD"
                     /protein_id="HRV97531.1"
                     /translation="MATIRVGIAESGVVLNPNSVSTLGLGSCVGVTLYDERRKIGGMV
                     HVMLPSTELAKGADFNRSKFADSAVPDLLQQMIRSGADQRRLVAKLAGGAQMFNLGGD
                     ADSLLKIGARNVEACKKALSLLRIPIVAEDTGGSWGRTVELFSESGVLEIRAIGKAKT
                     HI"
     gene            complement(1212..1529)
                     /locus_tag="P5201_03005"
     CDS             complement(1212..1529)
                     /locus_tag="P5201_03005"
                     /inference="COORDINATES: protein motif:HMM:NF016398.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheC"
                     /protein_id="HRV97532.1"
                     /translation="MGLSVLHEVGNIMIGSYITALSDFLGFELSPSVPSLAMDMAGAI
                     LAFSLSEIGRSGDTAIVIDGALSLDGGALSGNGAGHIFLLPDPESFNKIFQALGVAGN
                     GDH"
     gene            complement(1563..1907)
                     /locus_tag="P5201_03010"
     CDS             complement(1563..1907)
                     /locus_tag="P5201_03010"
                     /inference="COORDINATES: protein motif:HMM:NF016398.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheC"
                     /protein_id="HRV97533.1"
                     /translation="MTGERRESGNEGFRSPETGDALQRMSDFHLDVLREVSNIGAGNA
                     ATSLSTLLGKTVDMRVSRVFPMPFNEIIEYVGGAERMILSVFVRIEGGISGNMLFVMS
                     REDAQSLVRHMT"
     gene            complement(1904..2338)
                     /locus_tag="P5201_03015"
     CDS             complement(1904..2338)
                     /locus_tag="P5201_03015"
                     /inference="COORDINATES: protein motif:HMM:NF033749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriohemerythrin"
                     /protein_id="HRV97534.1"
                     /translation="MNIQWSDELAIGIGIIDDQHKKLIERIASFSHAVDGGDLHKVEE
                     TVNYLVGYAIQHFGAEELIMIRNCYDQFKEHRDEHSWFIKQVFDVHTSLVQKEECTQR
                     QIEDLRDMLYNWTLDHIMVKDKRIAQTLNSFGGPVRQGGGQA"
     gene            2483..3433
                     /locus_tag="P5201_03020"
     CDS             2483..3433
                     /locus_tag="P5201_03020"
                     /EC_number="6.3.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012869986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008716 - D-alanine-D-alanine ligase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--D-alanine ligase"
                     /protein_id="HRV97535.1"
                     /translation="MMITVLYGGEGPEREVSLRSGAAVTSAFAERGRDVTGADLRRKE
                     DLFALLDEGNVEFAFIALHGGWGEDGTLQAALEMAGVPFSGSGHAACALAMDKTASKA
                     LFRWKGISVPKGIEVARGSGARDVLDDPRFHPLLERSGKLVVKPCCSGSTVGVSILSG
                     TDRLAEALEDAFRYDPRVLVEEYVSGRELTVTVYEKEGVPRCLPVIEIQPVTGFYDYS
                     SKYTPGASEYLVPAPLSEFVLRRVEAASLAAHAALGCAAYSRVDLRLDEEGFPSVLEV
                     NTVPGMTATSLVPKAAAAAGISFGEFLEEVVESSLAARRG"
     gene            complement(3437..4705)
                     /gene="pfp"
                     /locus_tag="P5201_03025"
     CDS             complement(3437..4705)
                     /gene="pfp"
                     /locus_tag="P5201_03025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009200700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diphosphate--fructose-6-phosphate
                     1-phosphotransferase"
                     /protein_id="HRV97536.1"
                     /translation="MTNPDQKTLAIVCGGGPAPGINSVISSVTIEAKKSGWEVYGIYD
                     GFSNLGKGEKKVIPLTIDAVSRIHSDGGSILRISRFNPTKSDESLRKVVDTLIELGVT
                     HLVTIGGDDTAYAASRVADFAKNNLGLTINFVHVPKTIDNDLPLPEGIPTFGFETARS
                     LGTQIVSNLMEDAKTTGRWYLVVAMGRVAGHLALGIGKSAGATLTLIPEEFPGKEKIP
                     LSLVVDIVTGSIVKRLASGRNYGVAVTAEGLIEKIRLEDLEAADCLEYDDHGHIRYAE
                     INFSDVLKKELLVKLSGMGLKMTVNNKEVGYEVRCAPPNAFDIEYTRNLGYGAFEFLR
                     DGGTNALITIQNNQIVPIPFDQIIDPVTKKTRIRMVNTQSIQYRIARQYMIRLEKKDF
                     NPGIEFERLAVAAKMPPDEFRKQFQYVTDY"
BASE COUNT          904 a         1444 c         1492 g          974 t
ORIGIN      
        1 tctcgcggat gagcgcgagc ttcgggaaca gattctttcg gatgaatcga aggctgctcc
       61 gtccgctgta gaagtagccg aatatccaga cgaacatcac cagctgcgag atgaccgtcg
      121 ccacggcggc gcccttgatt ccccagccga acccgaagat gaaaagcggg tcgaggatga
      181 tgttgaggag tgcggagatg agcatcgtcg tcatcgcgat ccgcgcccgt ccctcggaac
      241 gcgccatgtg gttgcccgcc atggcgaaca tctggaacgg cacgccgaag agaatgaccg
      301 agaggtagtc ctccgcgtag gggagaatgc cctccgtcgc tccgaagagg cggagcagcg
      361 aaggaagaaa aaacagggag agcgaagaga cgagcagtcc taccgaaaga gagaatgcgg
      421 cggttgcgcc gaaggcttgc tgcgcgcggt cggtatctcc cgcgccgagg ctccgcgaaa
      481 cgacggaggc gccccctacc cccgccatga tggcgagcgc gccccccaga aactggatgg
      541 gaaaacagat catcaccgag gcgaggccga gcgggccgac gccgcgaccg atgaaaatgg
      601 agtccgcgat attgtacgag gccatgacaa gcatgccgat gaccgaaggc agggaaaggc
      661 gcagcaatag cttgccaacg ttctccgtgc cgagcagatg tttatcagtg ttcacgagat
      721 gcctccgaat ctttgttcaa atatgtgttt ttgccttgcc gatagcgcgg atttcgagga
      781 ctcccgactc ggagaacagt tccacggtgc gtccccacga gccgcccgta tcctcggcga
      841 caatagggat gcgaagaaga gagagcgctt ttttacaggc ttccacgttc cgggcgccta
      901 ttttcagcaa ggagtccgca tcgccgccga ggttgaacat ctgcgcgccg cccgccagct
      961 tggccacgag ccgtctctgg tccgcgccgg agcggatcat ctgctgcagc aggtcgggga
     1021 cggcggaatc ggcgaacttc gacctgttga agtccgcgcc cttggcgagt tccgtggaag
     1081 gaagcatcac atggaccata ccgccgattt ttcttctttc gtcgtagagg gtaacgccga
     1141 cgcacgagcc caaacccagg gtcgagacgc tgttcgggtt cagtacgacc cccgactcgg
     1201 cgatgcccac cctaatggtc gccatttccg gcgaccccca gagcctggaa gattttattg
     1261 aacgattccg gatcgggaag caggaagata tgccccgctc cgttcccgga gagcgcgccg
     1321 ccgtccagcg agagcgcgcc gtcgatcacg attgccgtgt cgccgcttcg cccgatctcc
     1381 gagaggctga acgcgagaat tgctcccgcc atgtccatcg ccagcgacgg aacggacggg
     1441 ctcagctcga agccgaggaa gtcggagagc gccgtaatgt acgagccgat catgatgttc
     1501 cccacctcgt ggaggacgga gagccccatc tcggagaagc cctgctcttc ggtctcggtc
     1561 gctcacgtca tgtgccggac gagcgattgc gcgtcctccc ggctcataac gaagagcatg
     1621 tttccggaga tgcccccctc gatacgcaca aagacgctca gaatcatccg ctccgctccg
     1681 ccgacgtact cgataatctc gttgaagggc atcgggaata cgcgcgacac gcgcatatcg
     1741 accgtctttc ccaacagcgt ggagagagat gtggcggcgt ttccggcgcc gatgttgctg
     1801 acctccctga gcacgtcgag gtgaaagtcg ctcatccttt gcagcgcgtc ccccgtttcg
     1861 ggcgagcgaa acccttcgtt tccgctttcc cgccgttcgc ccgtcatgcc tggccccctc
     1921 cctggcgcac cgggccgccg aaactgttga gtgtctgcgc gatgcgcttg tccttcacca
     1981 tgatatgatc aagggtccag ttgtacagca tgtcgcggag gtcttctatc tgtcgctgcg
     2041 tacattcttc tttttgcacc aggctggtgt gcacgtcgaa aacctgcttg atgaaccagc
     2101 tgtgctcgtc tctgtgctct ttgaactggt cgtagcagtt gcggatcatg atcagctctt
     2161 ctgccccgaa gtgctgaatc gcgtacccca caagatagtt caccgtctcc tcgactttgt
     2221 gcaaatcgcc gccgtccacg gcgtgcgaaa aagaggcgat gcgctcgatc agcttcttgt
     2281 gctggtcgtc gatgatgccg atgccgatgg cgagttcgtc gctccactgt atgttcatgt
     2341 gtattcctcc cgtcaggggc gatagaaact tggcgggaaa acacccgtct gctgtattgt
     2401 accagaaaga agagcggaag aagactggtt tgtgtataat aatgggccag aatacggata
     2461 ttaaggaaag gattgaattc ccatgatgat aaccgttctc tacggcggag aaggccccga
     2521 gagggaggtc tccctcagaa gcggcgcggc cgtgacttcc gccttcgcgg agcgggggcg
     2581 cgatgtgaca ggtgcggatc ttcgccgcaa ggaggacctt ttcgcgcttc tcgatgaagg
     2641 gaacgtcgag ttcgccttca tcgccctcca cggcgggtgg ggagaggacg gtacgctcca
     2701 ggcggcgctg gagatggccg gggttccgtt ctccggctcc ggacacgccg cctgcgccct
     2761 tgcgatggac aagacggcga gcaaggcgct ctttcgctgg aagggcatct ccgtaccgaa
     2821 ggggatcgag gtcgctcgtg gaagcggcgc ccgggacgtg ctggacgatc ctcgtttcca
     2881 ccctcttctg gaacgctcgg ggaaactcgt ggtcaaaccg tgctgttcgg ggagcactgt
     2941 cggcgtctcc atcctctcgg gaaccgaccg gctcgccgag gcgcttgaag atgcttttcg
     3001 ctacgatccc cgggtgcttg tggaggagta tgtctccggg agggagctga cggtgaccgt
     3061 gtatgagaaa gagggcgttc cccgttgcct gccggtcatc gagattcagc ccgtaactgg
     3121 attctacgac tacagctcga agtacacgcc gggagcctcc gaatacctcg ttccggcgcc
     3181 gctgtcggag ttcgtcctcc gcagggtgga agcggcctcc ctcgccgcgc acgcggcgct
     3241 cgggtgcgcg gcgtacagca gggtcgatct caggctcgac gaggaggggt tcccctccgt
     3301 tctcgaagta aacaccgttc ccgggatgac cgcgacgagc ctcgttccca aggccgcggc
     3361 ggcggcgggg atctccttcg gcgaatttct cgaagaagtt gtcgaatcct ccctcgccgc
     3421 ccggcgcggc tgagactcag tagtccgtca cgtactggaa ctgcttgcgg aactcgtccg
     3481 gcggcatctt cgccgccacc gcgaggcgtt cgaactcgat gccggggttg aaatccttct
     3541 tctccagccg gatcatgtac tgcctggcga tcctgtactg gatggactgt gtgttcacca
     3601 tgcggatacg ggtcttcttc gtgaccgggt cgatgatctg gtcgaacggg atgggcacga
     3661 tctggttgtt ctgaatggtg atgagcgcgt tcgtccctcc gtcgcggagg aattcgaacg
     3721 ccccgtaccc aaggttccgc gtgtactcga tgtcgaaggc gttcgggggc gcgcagcgga
     3781 cctcgtatcc gacttctttg ttgttgaccg tcatcttcag gcccatcccg gaaagcttga
     3841 cgagcagctc tttcttgagg acgtccgaga agttgatctc ggcgtagcgg atgtgcccgt
     3901 ggtcgtcgta ctccaggcag tccgcggcct caagatcctc caggcggatc ttctcgatca
     3961 gcccctcggc ggtgaccgcg acgccgtagt tgcgccccga ggcgagccgc ttcacgatgg
     4021 agcccgtgac gatgtccacc acgagggaga gcgggatctt ctccttcccc gggaattcct
     4081 cggggatgag cgtcaacgtc gcccccgcgc tcttgccgat gccgagggcg agatggcccg
     4141 ccacgcgccc catcgcgacc accaggtacc agcgccccgt cgtcttggcg tcctccatca
     4201 ggttggagac gatctgcgtc ccaagcgaac gcgccgtctc gaagccgaag gtggggatgc
     4261 cctcggggag cggaaggtcg ttgtcgatcg tcttggggac gtggacgaag ttgatggtta
     4321 gaccgaggtt gttcttcgcg aagtcggcga cgcgcgaggc cgcgtatgcg gtgtcgtcgc
     4381 ctccgatggt cacgaggtgc gtgactccca gctcgatgag cgtgtccacc accttccgca
     4441 gcgactcgtc gctcttcgtg gggttgaacc gggagatccg caggatgctc cctccgtcgg
     4501 agtggattcg gctgacggcg tcgatggtga gagggatgac cttcttctcc cccttcccca
     4561 ggttggagaa gccgtcgtag atgccgtaca cttcccaccc ggacttcttt gcttctatgg
     4621 tgacggagct gatcacgctg ttgatccccg gggcgggacc tcctccgcag acgatggcga
     4681 gtgttttttg atccggattc gtcatgaggc gcctccttca atatgaaaga taaaaagaaa
     4741 gacttcgtaa aattttagca catcgcacgg tataataaac ggcgaaggag cggatgcgca
     4801 tgatttttct tacc
//
LOCUS       DAOTIL010000092         4778 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminobacteriaceae bacterium isolate AMR_MDS_3026
            NODE_21182_length_4778_cov_3.088291, whole genome shotgun sequence.
ACCESSION   DAOTIL010000092 DAOTIL010000000
VERSION     DAOTIL010000092.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868050
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminobacteriaceae bacterium (wastewater metagenome)
  ORGANISM  Aminobacteriaceae bacterium
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae.
REFERENCE   1  (bases 1 to 4778)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4778)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:19:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,224
            CDSs (total)                      :: 3,198
            Genes (coding)                    :: 3,184
            CDSs (with protein)               :: 3,184
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4778
                     /organism="Aminobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21182_length_4778_cov_3.088291"
                     /isolate="AMR_MDS_3026"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:3034609"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2173)
                     /gene="lpxK"
                     /locus_tag="P5201_03030"
     CDS             complement(<1..2173)
                     /gene="lpxK"
                     /locus_tag="P5201_03030"
                     /EC_number="2.7.1.130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013048875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009029 - tetraacyldisaccharide 4'-kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetraacyldisaccharide 4'-kinase"
                     /protein_id="HRV97537.1"
                     /translation="MNGAGGVRRRFSPANVIQSIRSSVGVALGSYLSFARGETRFSPW
                     ILVYPFGLIARMVVAGRNFAFDHGLIRSEEPPLPVVSVGNITLGGTNKTPFVEMLCRM
                     FQEAGVTPGIISRGYGGRTVDPVVITAEEISAGAASEKTLSRYRDLVGDEPLLLASRL
                     PEVSVAVSKDRLRDVDVLSERNVQLIVADDAFQHRRMGRDADIVLVDACCPFGNGWIA
                     PAGILRESPSVLARASAVVVTKSDQVEPERLERLVGELSRFVPKERLFFSRISLLNWR
                     RWNGGWKDAAGERPDSVLAFSAIGSPESFRRSLEAGGVDILKEHRFKDHYRYRMEDMA
                     ALEASLEECGASCMVCTEKDVYNLPQEWRPTRDILVPFISTVLDEEARFRECLLEALR
                     PRMVVASNGYGEDSMGVLLARKLKERFPSASVSAFPIVGRGEHYMKEGIPIDSVPSDS
                     PSGGVIKYRFADLWRDLRSGLLRSIARQMGAWKLLRGRIRTPLCVGDVYLLLHALFGQ
                     GQLPVLIATAKTVYLSGHWRLERFLIKRRSRMAWTRDRDTAEELRRSGVQARFDGNPI
                     MDITCDNTIEPVSWGSENAPRILLLPGSRRRAYDDLVLLLQAVERIHAMLSEGASYLM
                     VVAPTLDTEKLLKACERVPATEEGQWTSFGGEHAPGVRKGTCEIRFFFGPLPAVAGRA
                     HLLVGLGGTANQVCAGMGVPVVSIEEKGKFVQKKLLGDSEVL"
     gene            complement(2170..3924)
                     /locus_tag="P5201_03035"
     CDS             complement(2170..3924)
                     /locus_tag="P5201_03035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014162710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HRV97538.1"
                     /translation="MTSEEKTASPSPYLRLLAYARPYSRRFFLALGCMVAASACNVVP
                     PWLLKNVVDDVLIAKNLAMLNALPFILVALFAGKGIASYGHQYLMNWVGQNVVMDLRL
                     RLYDHLQRMSLKYLYGKRFGELMSRITNDVTILQNLVTSVLVNLVVQAATFLGMIGFL
                     LYINWKLTLITFAILPLTVLILDRAAKKLRGVGHAIQSELATLSSVVQEAFGAIRIVR
                     SFATEDLELARFRNSNRDNFRAVMHGVQVQSALAGIIEVVLIAALALILWLGGKDVVG
                     GELTPGELIAFLGYLGFLVQPIRVFTSVVSSMQIGLAATDRIFALLDAPEDIVSPDNP
                     TPLGAIEGSVTFRHVCFSYVKGQEVLRGINLDVRPGEKIAIVGPTGSGKSTLADLIPR
                     FYDPDSGTVSVDGTDVRTVNLRELRKQIGVVHQDSLLLRGSIAFNISYGLPGLSLEEI
                     VKAAEIAGIHSFIESLPKGYDTEVGERGVTLSGGQRQRIAIARAVVRNPRILILDEAT
                     SSLDMEVERLVQKAMNKAMEGRTSFVIAHRLSTVRNADRIVVLDEGRIAEQGTHEELL
                     ARNGLYHRLCVLGETAAP"
     gene            complement(3921..>4778)
                     /locus_tag="P5201_03040"
     CDS             complement(3921..>4778)
                     /locus_tag="P5201_03040"
                     /inference="COORDINATES: protein motif:HMM:NF014714.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRV97539.1"
                     /translation="APLFCYSYGRKNGIERCAALFTAYREMAESIASAGEGQALQRTG
                     RILLAGDLAAELPDTFVHAPHGEDRIPEGRPRVAFFPGSREHIREYAVPLIRELAAAL
                     RESFPALDARIILSPFASAGEEEAFRRSGDLTPVRGGSKESLRGLDFAVTQPGTNTLE
                     LMHCAVPFLVAVPFSSLRHIPLPGVAGALANIPLIGPALREAALKAKGRRTGFVAWPN
                     RLAGRQVVDELIGDISARDVARVVGGHLLDGEYLARTRKSLAALSSSAPRNAAVFMAD
                     HIERMLAER"
BASE COUNT          960 a         1505 c         1482 g          831 t
ORIGIN      
        1 caagcacctc ggaatcgccg aggagcttct tctggacgaa cttccctttc tcctcgatgg
       61 agaccacggg aacccccatc cctgcgcata cctgatttgc ggtccctccg agcccgacaa
      121 gaaggtgcgc tctgccggcc accgccggaa ggggaccgaa gaaaaagcga atttcacagg
      181 ttcctttccg tactcccggc gcgtgttctc cgccgaaaga agtccactgt ccttcctccg
      241 tcgcgggaac gcgctcgcag gctttcagca gtttttccgt gtcgagggtg ggcgcgacga
      301 ccatgaggta ggacgctcct tccgaaagca tagcgtgtat gcgctctacg gcctgcaaca
      361 ggagaacaag atcgtcatac gcccgtcgcc tgctcccggg aagaagcagg atacgcggcg
      421 cgttctcact tccccaggag accggctcga tagtattatc acacgtaatg tccattatgg
      481 gattaccgtc aaagcgagcc tgaacgcctg agcgccgcag ctcctcggcc gtatcccggt
      541 cgcgggtcca cgccatccgg ctcctgcgtt tgatgaggaa tcgttcgagc ctccagtgac
      601 cgctgaggta gaccgtcttc gccgtcgcga tgaggacggg gagctgcccc tgtccgaaga
      661 gcgcgtggag caacaggtag acgtcgccga cgcacaacgg ggtcctgatt cgcccgcgga
      721 gcagcttcca cgcccccatc tggcgggcta tggaacgaag aaggccggag cgcagatccc
      781 gccagaggtc ggcgaaccgg tacttgatca ctccgccgct cggcgagtcg gagggaacgg
      841 agtcgatcgg gattccctcc ttcatgtagt gctcgcccct tccgacgatg gggaatgccg
      901 aaaccgacgc ggacggaaaa cgctccttga gcttcctggc gagcagcacg cccatcgagt
      961 cctcgccgta cccgttggac gcgaccacca tccgggggcg cagggcttcc agaaggcact
     1021 ccctgaatcg cgcctcctcg tcgagcacgg tggagatgaa cggaaccaga atatcacgtg
     1081 ttgggcgcca ctcctgaggg agattgtaga cgtccttttc ggtacagacc atgcacgacg
     1141 cgccgcactc ctccaaagaa gcctccagcg cggccatgtc ctccatccgg tagcggtagt
     1201 gatctttaaa gcggtgttcc ttcagaatgt cgacgcctcc ggcttccaaa gaacggcgaa
     1261 agctctccgg actgccgatg gccgagaacg cgagcaccga gtccggtcgt tctccggccg
     1321 cgtctttcca ccccccgttc caacgcctcc agttgagcag cgagatgcgt gagaagaaca
     1381 gccgttcctt cgggacgaag cgggaaagtt ccccgacaag acgctccagg cgctcgggtt
     1441 ctacctggtc cgacttggtg acgacgacgg cggacgccct cgcaagcacc gacggcgatt
     1501 ctctgagaat accggccggc gcaatccatc cgttgccgaa cgggcagcac gcgtcgacaa
     1561 gcacgatgtc cgcgtcgcgc cccatccttc ggtgctgaaa ggcgtcgtcg gccacgataa
     1621 gttgtacgtt ccgctcggaa aggacgtcga cgtcgcgcag acgatctttg gagaccgcaa
     1681 cggaaacctc cgggaggcgc gaagccagaa gaagcggttc gtcgccgaca agatctctgt
     1741 atcgggagag cgtcttttcg gaagccgccc cggcggatat ctcctccgca gttattacta
     1801 caggatcaac ggttcgcccc ccgtaccccc tgctgatgat accgggcgtc acgcccgcct
     1861 cctggaacat acgacagagc atctcgacga acggcgtctt gttcgtccct cccagcgtga
     1921 tgttcccgac gctcaccaca ggaagcggag gctcctccga gcggatcagg ccgtgatcga
     1981 aagcgaaatt tcggccggcg acaaccatcc tggcaatcag gccgaacgga taaacaagaa
     2041 tccacgggga aaagcgcgtc tctccgcggg cgaaggagag gtagcttccg agggcgacgc
     2101 cgaccgaaga gcggatgctt tggatcacat tcgccggaga gaaacgcctt cgcacgcctc
     2161 ccgcgccgtt catggagcag ctgtctcccc gaggacgcac aggcggtgat agagaccgtt
     2221 gcgcgcgagc agctcttcat gcgtcccctg ctcggcgatg cgcccttcgt caagcacgac
     2281 gatcctgtcc gcgttccgga ccgtggagag gcgatgcgcg atgacaaagg aggtgcgtcc
     2341 ctccatggcc ttgttcatcg ccttctggac cagacgctcg acctccatgt cgagagagga
     2401 cgtcgcctcg tcaaggatga ggatcctcgg attccgtacc acggcccggg ctatggcgat
     2461 gcgctgccgc tgtccgccgc tgagagtaac cccgcgttcg ccgacttcgg tgtcgtaccc
     2521 tttagggagc gattctatga aggagtgaat acccgctatc tccgcggctt tgacgatttc
     2581 ctccagggag agtccgggca ggccgtacga tatgttgaag gcgattgagc cgcgcaacag
     2641 cagcgaatcc tggtgcacca cgccgatctg ctttcgaagt tcccgaagat tgacggtgcg
     2701 cacatccgtg ccgtcgacgg agaccgtccc gctgtcggga tcgtagaagc ggggaatgag
     2761 atcggccagc gtggatttgc ccgagcctgt cgggccgacg atggcgatct tctccccggg
     2821 acggacgtcg aggttgatcc ccctgaggac ttcctgcccc ttgacgtacg aaaaacaaac
     2881 gtgcctgaag gtgacgcttc cctcgatcgc cccgagcggc gtcgggttgt ccggggagac
     2941 gatgtcctcc ggcgcatcga gcagggcgaa gatcctgtcc gtggcggcaa gccctatctg
     3001 catggaactg accaccgagg tgaacacacg aatgggctgc acaaggaacc ccagatagcc
     3061 gaggaaggcg atcagttcgc cgggagtgag ctcgcccccg acgacgtcct tgcctccgag
     3121 ccagaggatg agcgcgagcg ccgcgatgag gacgacctct atgattccgg cgagggccga
     3181 ctgcacctgg acgccgtgca tcactgcgcg gaagttgtcg cggttgctgt tcctgaaccg
     3241 ggcgagctcc agatcctcgg tcgcgaaaga acgcacgatc cggatcgcgc cgaatgcctc
     3301 ctgcacgacc gaggagagcg tcgccagctc gctctgtatg gcgtgcccca ccccgcggag
     3361 cttcttcgcg gcacggtcca ggatgagcac ggtaagcggg agaatagcga aggtgataag
     3421 cgtgagcttc cagttgatgt acaggagaaa gccgatcatt cccaggaagg tcgccgcctg
     3481 cacgacaaga ttgaccagta ctgacgtgac caggttctgg aggatggtca cgtcgttcgt
     3541 gatacgcgac atcagctccc cgaaacgctt gccgtagagg tacttcagcg acatccgctg
     3601 gagatggtcg tagagccgaa ggcgtaaatc catcacgacg ttttgcccta cccagttcat
     3661 caggtactgg tgcccgtacg aggcgatccc tttcccggcg aagagcgcca cgaggatgaa
     3721 aggaagcgcg ttgagcatgg caaggttctt ggcgatcagg acgtcgtcca cgacgttctt
     3781 gagcagccac ggcggcacga cgttgcaggc cgaggccgca accatgcacc ccagcgcaag
     3841 gaagaacctg cgcgagtagg ggcgggcata ggcgagcagc cgaagatacg gagaagggga
     3901 agcggttttc tcttcggagg tcatcgttca gcaagcatcc tttcaatatg atcggccatg
     3961 aatacggcgg cgttccgggg cgcggaggaa gagagcgcgg cgagcgactt cctcgtccgg
     4021 gcgaggtact cgccgtccag aagatgcccc ccgacgaccc gggcaacatc ccgcgcagag
     4081 atatcgccga tcagttcatc aacaacttgg cgccctgcga ggcggttcgg ccaggccaca
     4141 aaccccgtcc ggcgcccttt cgccttgagc gcggcttcgc gcagcgcagg gccgatcagc
     4201 ggaatgttgg ccagcgctcc ggcgacgccg ggcagcggaa tatggcgaag tgaagaaaac
     4261 ggaaccgcga cgagaaaggg aacggcgcag tgcatcagtt cgagcgtgtt cgttcccggc
     4321 tgcgtcacag cgaagtcaag gccccggagc gactctttgc ttcctcctcg caccggggtc
     4381 aggtctccgg agcgacggaa cgcttcctcc tccccggccg aggcgaaggg agaaagaata
     4441 atgcgggcgt caagcgcagg gaacgactct cgcagcgccg cagcaagctc gcgtatcagc
     4501 ggcacggcgt actccctgat gtgttcccgg cttccgggga agaaggcaac tcttggacgc
     4561 ccctcaggta tgcggtcttc gccgtggggc gcgtgcacga aagtatccgg cagctcggcg
     4621 gcaagatcgc ccgccagaag aattcgtcct gtccgttgca gcgcttgccc ctcgccggcg
     4681 gaggcgatcg actccgccat ctctctgtag gcggtaaaga gggcggcgca gcgttctatc
     4741 ccgttttttc gcccgtacga gtaacagaag agcggcgc
//
LOCUS       DAOTIL010000093         4773 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminobacteriaceae bacterium isolate AMR_MDS_3026
            NODE_21214_length_4773_cov_3.838703, whole genome shotgun sequence.
ACCESSION   DAOTIL010000093 DAOTIL010000000
VERSION     DAOTIL010000093.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868050
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminobacteriaceae bacterium (wastewater metagenome)
  ORGANISM  Aminobacteriaceae bacterium
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae.
REFERENCE   1  (bases 1 to 4773)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4773)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:19:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,224
            CDSs (total)                      :: 3,198
            Genes (coding)                    :: 3,184
            CDSs (with protein)               :: 3,184
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4773
                     /organism="Aminobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21214_length_4773_cov_3.838703"
                     /isolate="AMR_MDS_3026"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:3034609"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..351)
                     /locus_tag="P5201_03045"
     CDS             complement(<1..351)
                     /locus_tag="P5201_03045"
                     /EC_number="4.3.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012868827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008851 - ethanolamine ammonia-lyase
                     activity [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine ammonia-lyase light chain EutC"
                     /protein_id="HRV97540.1"
                     /translation="MVTELELKQMVQQILVEMSGKESVAQKVGPLPVQSASSDDGDCL
                     PDLTEIDLRKQCLVPAPADAEALMAMKLSTPARIGVWRAGPRYKTETLLRFRADHAAA
                     QDAVFSHVSDEFLAA"
     gene            complement(356..1729)
                     /locus_tag="P5201_03050"
     CDS             complement(356..1729)
                     /locus_tag="P5201_03050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008710661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine ammonia-lyase subunit EutB"
                     /protein_id="HRV97541.1"
                     /translation="MNLKTKLFGRVYQFKNVKEVLAKANEEKSGDRLAGIIAESMEER
                     IAAKHVLANLTVADLRNNPVIPYEQDEVTRIIQDDVNERIYSEIAQWTLSDLREWILC
                     DETSGDDIKRVSRGLTSEVVAGVAKLMTNLDLVYGASKIRIQAHCNTTIGLPGTLSAR
                     LQPNHPTDDIEGVTLSVFEGLSYGVGDAVIGLNPVDDTVDNVKKILFKFQEIKEKFEI
                     PTQMCVLAHVSTQMEAVRQGAPTDLFFQSVAGSQKGNEAFAISGDLLSEAMELAAKQG
                     TGVGPNFMYFETGQGSELSSDSHFGADQVTLEARCYGFAKRFKPFMVNTVVGFIGPEY
                     LYDSRQVIRAGLEDHFMGKLTGISMGCDACYTNHMKADQNDIENLAVLLTSAGCNYFM
                     GVPHGDDVMLNYQCTGFHEAAALRQLLRVRPVAAFEKWLEKMGIMENGILTKKAGDAS
                     IFCVRGG"
     regulatory      complement(2031..2202)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00174"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="cobalamin riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="adenosylcobalamin"
                     /db_xref="RFAM:RF00174"
     gene            2702..3904
                     /locus_tag="P5201_03055"
     CDS             2702..3904
                     /locus_tag="P5201_03055"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012869981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004072 - aspartate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate kinase"
                     /protein_id="HRV97542.1"
                     /translation="MWKDGEGVVLKFGGSSVATPERIRAAARRVASFARQGRRTAVVV
                     SAMGKTTDDLLGLARATAPSAGDRRELDRLLATGEQQSAALLAMALSAEGCPARSFSG
                     DEAGFVAEGAWGEGRILSVDPARIEAATAFGHVAVITGFQACTRDGETITLGRGGSDL
                     SAIALAAALGAAECRIFTDVDGIYTADPRVVPGAVKLDRIPWDECLEMAFCGAAVMQA
                     RSIEMAGRMSIPLCVASSFQESEGTWIMERNVDEAVFIRSVASDDDLAVLTVEGRTVE
                     RELMDALSGEGIRTTAMVGAAGASFHIEGARLPEAVEICRKLAGCSVSVNETLSRVSV
                     IGPGAGNHPEVCRAMLGILAEMGATVHLLTSSALSVTCVVDRDRSAEAVRALHEEFIG
                     KKGVFQCA"
     gene            3895..>4773
                     /locus_tag="P5201_03060"
     CDS             3895..>4773
                     /locus_tag="P5201_03060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013047896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine dehydrogenase"
                     /protein_id="HRV97543.1"
                     /translation="MRVDSSLGLCFIGFGNVGQGLARILVRKERELAERWGFTFRTVA
                     VVTRTRGAAVSRDGEGLSLADLLARVERGDPVGNERISPVEAALLPQTDIVLDVTPTN
                     LINGEPGLSLTRAALGSGRSVVSSNKGPVSLALAELKELARARGAHYRFEGSVMSGTP
                     SLNLALESLAGCDVREVQGIVNGTTNYILTRMEEGFEYADALREAQEKGYAEADPAGD
                     VDGWDAAVKAQILAAAVLGFPLPLDAVERTGIAAISRADVEAAAKRGKRIKLLAKAAR
                     TPDGVAASVAPAEIPLS"
BASE COUNT          837 a         1575 c         1488 g          873 t
ORIGIN      
        1 cgccgcgagg aactcgtcgg atacgtggct gaacaccgcg tcctgcgccg ccgcgtggtc
       61 cgcccggaag cggagcagcg tctccgtctt gtaacggggc cccgcgcgcc atacgccgat
      121 acgcgcagga gtcgacagct tcatcgccat cagcgcctcg gcgtccgccg gagcggggac
      181 aaggcactgt tttctcagat cgatctccgt cagatcgggg agacagtctc catcgtcact
      241 ggaagcgctc tgcactggca ggggcccaac cttttgggca acgctttcct tcccgctcat
      301 ctccacaaga atctgctgga ccatttgctt gagttcaagc tcggttacca taatctcagc
      361 ctcccctcac gcagaagatg gacgcgtctc cggccttctt cgtcaggatt ccgttctcca
      421 tgattcccat cttttcaagc cacttctcga acgcggcgac cggacggacg cggagcagct
      481 gacgcagcgc cgccgcctcg tggaaaccgg tgcactggta gttcagcatg acgtcgtcgc
      541 cgtggggaac tcccatgaag tagttgcacc ccgccgaggt gagcaggacg gccaggtttt
      601 cgatatcgtt ctggtcggcc ttcatgtggt tggtgtagca ggcgtcgcac cccatggaga
      661 tgccggtcag cttgcccatg aagtgatctt ccaatcccgc gcggatgacc tgccggctgt
      721 cgtacaggta ctcggggccg atgaagccga cgaccgtgtt caccatgaac ggcttgaagc
      781 gcttcgcgaa gccgtagcag cgggcctcca gcgtcacctg gtcggcgccg aagtgagagt
      841 cggacgacag ctcggagccc tgccccgtct cgaagtacat gaagttcggg ccgacgcctg
      901 ttccctgctt cgcggccagc tccatcgcct cgctcagcag atcgccggag atggcgaacg
      961 cctcgttccc cttctgggaa ccggcgacgc tctggaagaa gaggtcggtc ggagcgccct
     1021 ggcggacggc ctccatctgg gtgctcacgt gcgcgaggac gcacatctgg gtgggaattt
     1081 caaacttttc cttgatctcc tggaatttga agaggatttt cttgacgttg tcgaccgtgt
     1141 cgtcgacggg gttcagaccg atcaccgcgt cgcccactcc gtaagagagg ccttcgaaga
     1201 cggagagcgt cacgccttcg atatcgtcgg tggggtggtt gggctgcagg cgggcggaaa
     1261 gcgtccccgg aaggcctatc gtggtattgc agtgcgcctg gatacggatc ttgctcgctc
     1321 cgtagacgag gtcgaggttg gtcatcagct tggccacgcc cgctaccact tcgctggtga
     1381 gtccgcggct gacccgcttg atgtcgtctc cggaagtttc gtcgcacagg atccactcgc
     1441 gcaggtcgga gagcgtccat tgtgcaattt cggagtaaat ccgttcgttc acgtcgtcct
     1501 ggatgatacg ggtgacttcg tcctgttcgt acgggatcac cggattgttc cggagatccg
     1561 cgacggtcag gttggcgagc acgtgcttcg ccgcgatccg ttcctccatc gactccgcga
     1621 tgattccggc gaggcggtcg ccggacttct cctcattcgc cttggcaaga acctctttga
     1681 catttttaaa ctggtacaca cgaccaaaga gcttggtttt gaggttcacc gtcatttcac
     1741 ctccaatgtg ttttcatgcg tcctcacgcg ccggagcccc gaagagcggc caggaacgcg
     1801 gacggacgtg cgcaaccggc gatacgtctc cgggaacgga ccgcccccga agagcatacg
     1861 gcatcagtgc aactctcccg aaccaccccc ctttcgtagc aatacggcgc cgcgcgcttc
     1921 ccgcacccgg ggaatttcgc gtacaaaaaa agacccgggg cgcatccgcg aaaaacaccg
     1981 gtgttcaccg ccggtcttct ccgtaaacgg cctcaagtcc ccgcaaggcg tttctgcccg
     2041 atgcgcgcaa tcggtctcct ggcttgcctt catcctcaga ccgctccttc ccggaagact
     2101 ccggcggaac cccgcggctt cgtcaggcgt acagtggcgg gcccgcgccg gactcttacc
     2161 ggcttcccga gcatcgcgcg cacctcgaca aaaaaataaa ctagagcaac tttttcacca
     2221 gcaatcagag tatatcatgg atagaaaaat gtcaacggtt cttcacaaag agtcaacaga
     2281 agtcctccat ttagaaataa tttctatata atttaaaaat cgacatcctt gtaaaggcag
     2341 acaagactcc tgccacccgt tatttcccct ccccgcccgc tcttgcagcg acggagaggg
     2401 gaaagggagg cgcgcatttt tccaccctgc ggattttttt cacttcctgc gcttgacaag
     2461 tcaccggaag gggcgtatta tcctgcccaa cctaaaccga ttcgaaagga ggtccccgca
     2521 ctatgaccaa tgcgaaaaag atccgcgcgg cagttctgtt cgcatgtgca atgacgatga
     2581 ctagcggcga ccctcctgga gcggtcggcg cttagcaccg cgtgcgcgat agcttcagcc
     2641 gggggtcttc gtagatgaag gcgcccggct ttttttgtac gcataaggga ggtcggcggt
     2701 tgtgtggaaa gatggagaag gggtagtact caagttcggc ggctcgtccg tggcgacgcc
     2761 tgaacggatt cgcgccgcgg cgcggcgtgt cgcctccttc gcccggcagg ggcgcaggac
     2821 ggcggtcgtc gtctccgcca tgggaaagac caccgacgat cttctggggc tggcgagagc
     2881 gaccgctccg tcggccggcg accggcgcga gctggacagg ctgctggcca cgggagagca
     2941 gcagtcggcc gcgctgcttg cgatggccct ctccgccgag gggtgtccgg cgcgctcgtt
     3001 cagcggcgac gaggccggtt ttgtcgccga gggcgcgtgg ggagaggggc gcatcctctc
     3061 ggtcgacccg gcgcggatcg aggcggccac cgcgttcggt cacgtcgccg tcatcaccgg
     3121 ctttcaggcg tgcacgcggg acggcgagac gatcaccctc gggcgcggcg gctcggacct
     3181 ctccgccatc gccctggccg cggccctcgg ggcggccgaa tgccgcatct tcaccgacgt
     3241 cgacggcatc tacacggcgg acccgcgcgt cgtccccggc gcggtcaagc tcgaccggat
     3301 cccctgggac gagtgcctcg aaatggcctt ctgcggcgcc gcggtcatgc aggcgcgcag
     3361 catcgaaatg gcgggcagga tgagcatccc cctgtgcgtc gcatccagct ttcaggaaag
     3421 cgagggaaca tggatcatgg aacgaaacgt ggatgaagct gtctttatca ggtcggtcgc
     3481 atccgacgac gaccttgcgg tgctcaccgt ggaggggaga acggtcgagc gcgaactgat
     3541 ggacgcgctc tccggagagg gcatccgcac caccgcgatg gtgggcgcgg ccggcgcatc
     3601 cttccacatc gaaggggcgc gcctccccga ggcggtcgaa atctgccgga aactggccgg
     3661 atgcagcgtc tcggtcaacg aaacgctctc ccgcgtctcc gtgatcggac ctggcgcggg
     3721 gaaccacccc gaagtgtgcc gcgccatgct cggcattctg gcggagatgg gcgccaccgt
     3781 ccacctgctc acctcgtcgg cgctctccgt cacctgcgtc gtggacaggg accgctctgc
     3841 ggaggccgtc cgggcgcttc atgaagaatt catagggaag aaaggggtat tccaatgcgc
     3901 gtagactctt ctttgggact ctgcttcatc ggcttcggca acgtcgggca ggggctcgcc
     3961 cgcatcctcg tccgcaagga gcgcgaactt gcggaacgct ggggcttcac cttccgcacc
     4021 gtcgccgtcg tcacccgcac caggggcgcg gccgtctcgc gcgacgggga agggctctct
     4081 cttgcggacc tgctcgcccg cgtcgaaagg ggcgaccccg tcgggaacga acgcatctcc
     4141 cccgtcgagg cggccctcct tccacagacc gacatcgtcc tcgacgtcac gcccaccaac
     4201 ctgatcaacg gcgagccggg gctgtcgctc acccgcgccg cgctcgggtc ggggcggtcc
     4261 gtcgtcagct cgaacaaggg ccccgtctcc ctcgccctcg ccgaactgaa ggagctcgcc
     4321 cgagcgcggg gcgcgcacta ccgcttcgaa ggctccgtga tgagcggcac cccgtcgctc
     4381 aacctcgccc tcgaatcgct cgcggggtgc gacgtgcgcg aggtgcaggg gatcgtgaac
     4441 ggcaccacca actacatcct cacccgcatg gaagaagggt tcgagtacgc cgacgctctc
     4501 cgcgaggcgc aggagaaggg gtacgccgag gccgacccgg ccggagacgt cgacggatgg
     4561 gacgccgccg tgaaggccca gatcctcgcc gccgccgtcc tcggcttccc gctgccgctc
     4621 gacgccgtgg agcgcaccgg catcgccgcc atctcccgcg ccgacgtgga ggccgccgcg
     4681 aagcgcggca agcggatcaa gctgctggcg aaggccgccc gcacgcccga cggcgtggcc
     4741 gcctccgtcg ccccggcgga aattcccctc tcc
//
LOCUS       DAOTIL010000094         4703 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminobacteriaceae bacterium isolate AMR_MDS_3026
            NODE_21646_length_4703_cov_3.658133, whole genome shotgun sequence.
ACCESSION   DAOTIL010000094 DAOTIL010000000
VERSION     DAOTIL010000094.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868050
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminobacteriaceae bacterium (wastewater metagenome)
  ORGANISM  Aminobacteriaceae bacterium
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae.
REFERENCE   1  (bases 1 to 4703)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4703)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:19:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,224
            CDSs (total)                      :: 3,198
            Genes (coding)                    :: 3,184
            CDSs (with protein)               :: 3,184
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4703
                     /organism="Aminobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21646_length_4703_cov_3.658133"
                     /isolate="AMR_MDS_3026"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:3034609"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2331)
                     /locus_tag="P5201_03065"
     CDS             complement(<1..2331)
                     /locus_tag="P5201_03065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008708913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HRV97544.1"
                     /translation="MFSKFFIDHPRFAMVIAIVLTMAGIISAFNLPVKQYPDVAPPQI
                     NVSATYSGADAETLANAVGVPLEEAINGVDDMIYMQSTSSSTGRYSLTITFKTGTDTD
                     MALVKVQNRVQQATPLLPSEVTASGITTESRFSNTLGFVALISPNGTRDSLFLADYAY
                     NNVSKGLKRVPGLGAVEVLGAKYSIRVWLDPERLASMGLSTTDVANAITSQNRQASIG
                     SIGSAPGNTDTPLVYTLTTTGRLGSVRQFENIILRTTAEGGLVKLKDVSRIELGSENY
                     AFRGALNGSPSAVMLLSQASGSNALDAMTATREALADMEKDLPDDTAFLIGYDTTDFV
                     RSTISEILLTLALTFSLVVLVCYVFLQDWRVTLVPVMAIPISLLATFTGLMLLGFSIN
                     ILTLFAFVLVIGTVVDDAIIVVERVMYVMERDKTNSVDATIQAMKDVTGPMTATTLVF
                     LAIFVPVAFMGSITGEIYRQFAVTVSFSVVFSLIVALTLSPAMCAHMLGNVRPKTTGP
                     LAWFNTMVSRASSKYVAGSMWIARRTIVTLGLLVLVLVTSVGIMKITPTAFVPDEDQG
                     AVFAVVQLPEGASQGRTQAVMDKIVPRFSDIPGVKDVIDIIGFSIMGGSGENVGSVIL
                     TLDNWSLRDSKELSQDSILGKIRAVGGSIPEAKISVFAPPTISGLGIAGGLDIRLQAT
                     QDADPQKLSMVLGEFLGKIFQAPEFSYAFSAYTSDTPHLDLDIDREKAESLGVPVSSI
                     FRTLQTYFGTTYINDINIGATVNKVIVQADWPFRNKI"
     gene            complement(2355..3626)
                     /locus_tag="P5201_03070"
     CDS             complement(2355..3626)
                     /locus_tag="P5201_03070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008708915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HRV97545.1"
                     /translation="MTSSEQYKNAGSGSKPNFRMVKTIIGILLIAVIVFFGRSYLSGR
                     SDGQQVPMQPQAPAVVLYTVEKTDLSVGREYVGRVEPIQAVSLKPQVSGEIEKVHFKE
                     GSMVKAGDLLFTLDRREYRATVALRKAELAKAEANYDRVLKYNERLKAADKRSVSASD
                     IELSESDVLQGKAAVEQAKATLRLAEIDLGYTRITAPISGQAGKALFTKGNYVTPSSG
                     ELTTIVQKNPIRVTFSLPDKEYLDQIETFRASGESVYDATIRLSNGKLYPAKGSRDFE
                     NNVMDDRTGSMMISLRFENAQGVLVPGAMVRVLAKPAKSRVVSIIPQEAILADAEGDF
                     VYFVGSDDIAAQRRVKLGDEFGTMREVISGLEQGEKIIFRGLQAVRPGAKVKPISGTN
                     GNASKTPAELAMESDSDPKTITSGDAGTDTN"
     gene            complement(3639..4274)
                     /locus_tag="P5201_03075"
     CDS             complement(3639..4274)
                     /locus_tag="P5201_03075"
                     /inference="COORDINATES: protein motif:HMM:NF012655.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR family transcriptional regulator"
                     /protein_id="HRV97546.1"
                     /translation="MVRRVREEALETRKQLLEAALEIMSEKPFSSVSMTEIARKIGLS
                     KGAIYWHFKNKNDVLLHVILNIRTQMAEEFCADSASLESIDDMRTYYKKKMQKPLQDE
                     EFKKIHRLMHRGEEWPEEIRKKVLEMILECLDLEQKMTERLLVRAQEEGTIRMDVSPR
                     DLSLMVTAMFQGIFVFQVHDFYHVDLTEHIDFIFDAFKKELDSANNTKQKG"
     gene            4452..>4703
                     /locus_tag="P5201_03080"
     CDS             4452..>4703
                     /locus_tag="P5201_03080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009200863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HRV97547.1"
                     /translation="MAFKGELDSVKIVTVLDDYAGYETSFLAQHGIALLLEACSNGVC
                     KRILMDAGQSALPILHNMKLMDIDPASIDAVIISHCHYDH"
BASE COUNT         1010 a         1293 c         1210 g         1190 t
ORIGIN      
        1 tatcttgttc ctaaaaggcc agtcggcctg cactatgacc ttgttcaccg ttgctcctat
       61 attgatgtcg ttgatgtatg ttgtgccgaa gtatgtctgc agtgtcctga aaatgcttga
      121 gacgggaacc cctagacttt ccgctttttc cctgtcgata tcgagatcga gatgaggcgt
      181 gtcggaggta taggcgctga aagcgtagct gaactcgggc gcctggaaga tctttccgag
      241 gaactcgccg agaaccatgg aaagtttttg cgggtcggca tcctgtgttg cctgcaggcg
      301 aatatccagg cctccggcga tacctaaccc gctgatcgta ggaggcgcga aaacgctgat
      361 ctttgcctcc ggaattgaac ctccgacggc gcggatcttt cccaggatgc tgtcctggct
      421 cagttccttc gaatcccgga gagaccagtt atcgagggtc aggatgacgg atccgacgtt
      481 ttccccggag cctcccatga tgctgaatcc gattatatcg ataacgtcct tgaccccggg
      541 gatatcgctg aaccgcggaa ctatcttgtc cattacggcc tgcgttctcc cctgtgatgc
      601 tccttcggga agctgtacga cagcgaaaac agctccctgg tcctcgtccg gaacaaatgc
      661 cgtcggtgtg atcttcatga tcccgaccga agttacaagc accagtacga gaagcccgag
      721 cgtaacgatc gttctgcgcg ctatccacat ggaacctgca acgtacttgc tcgaggccct
      781 ggacaccatg gtgttgaacc aggccagcgg ccctgtcgtt ttcggcctga cgttgccgag
      841 catatgcgcg cacatggcag ggctgagcgt cagtgcgaca ataagagaga agacgacgga
      901 gaaggataca gttacggcga actggcggta tatttcaccc gttatgcttc ccatgaacgc
      961 gacgggaacg aatatcgcaa gaaagaccag cgtcgtggcg gtcatgggtc cagtaacgtc
     1021 tttcatggcc tggatagtcg catcgacgga gttggtcttg tccctttcca ttacgtacat
     1081 gacccgttcg acgacgatga tcgcgtcgtc gacgacggtg ccaatgacga gaacgaacgc
     1141 gaacagggtg aggatgttga tgctgaaacc aagcagcatc aggcctgtaa aggtggccag
     1201 gagtgaaatg ggtatcgcca tgacgggaac aagggtgacc cgccagtcct gcaggaagac
     1261 atagcagacc aggacgacca gggagaacgt aagagctaga gtaaggagga tctccgagat
     1321 cgtcgacctc acgaagtcag tggtgtcata tcctataaga aaggccgtgt cgtcaggcag
     1381 gtccttttcc atatcggcca gggcttctct tgttgcggtc attgcatcga gagcattcga
     1441 tccggacgcc tgcgagagca gcattactgc agaaggagat ccgttgagag cgccgcgaaa
     1501 ggcataattc tcggacccga gctctattct cgatacgtcc ttaagcttga caaggccacc
     1561 ttcggctgtc gttcgcagaa tgatgttctc aaactggcgt acgcttccaa ggcgtccggt
     1621 agtcgtcaat gtatagacca gcggcgtatc tgtgttcccc ggagccgatc cgatcgaacc
     1681 gatggaggcc tgcctgttct gactcgttat cgcattggcg acatccgttg tgctgagtcc
     1741 catcgatgca agacgttccg ggtcaagcca gacacgaatg ctgtatttcg ctcccagaac
     1801 ttcaacagcg cccagacccg gcacgcgctt caggcccttg cttacattgt tgtaggcata
     1861 atccgccagg aagagcgaat ctctcgttcc attcggcgat atgagggcta cgaacccgag
     1921 cgtgttcgag aatcttgatt ccgtcgtgat gccgcttgcc gtaacctcac tcggcagaag
     1981 gggagttgcc tgctggacac ggttctggac cttgaccagc gccatgtccg tatccgttcc
     2041 cgtcttgaat gttatggtca gcgaatagcg ccccgtgctg ctcgatgtcg attgcatgta
     2101 gatcatgtca tcgacgccgt tgatcgcttc ttcaagggga acgccgacgg cattggccag
     2161 cgtttcggcg tcggcgccgg aatatgtggc cgacacattt atctgcggcg gtgcgacatc
     2221 gggatattgc tttacgggca ggttgaatgc cgagattatt cctgccatcg taaggacaat
     2281 ggctatgacc attgcaaatc ttggatgatc tataaagaac tttgagaaca tgggttattt
     2341 tctccttctg cgggttagtt cgtgtccgtg ccggcatctc cggacgttat tgtcttcggg
     2401 tcggaatccg actccatcgc aagctccgcc ggcgttttgg aagcattacc gttcgtaccg
     2461 gatatgggct tcacttttgc ccccggccga accgcctgca ggcccctgaa aatgatcttt
     2521 tctccctgct ccagtcctga gatcacttcg cgcatcgtac cgaattcatc gccgagctta
     2581 actctgcgct gggcggcaat atcgtccgaa ccgacgaaat atacgaagtc gccttcagca
     2641 tcggcaagga tcgcttcctg cggtatgatc gagacaacgc gtgacttcgc cggttttgcc
     2701 aggacccgga ccatggctcc cgggacaagg acaccctggg cattttcaaa gcgcaggctt
     2761 atcatcatgc tccccgtcct gtcgtccatt acgttgtttt cgaagtcgcg cgatcctttt
     2821 gccgggtaca gcttcccgtt tgaaagccgg atcgttgcat cgtagacgga ttcgcccgaa
     2881 gccctgaatg tctcgatctg gtcgaggtac tccttgtcgg gaagtgagaa agtgacgcgt
     2941 atggggtttt tctgaacgat cgtagtaagt tccccgcttg acggcgttac ataatttccc
     3001 ttcgtgaata aagccttgcc tgcctgccct gagatcggcg ccgtgatcct tgtatatccc
     3061 agatcgatct ccgcgagccg gagcgtcgcc tttgcctgtt ccacggcggc cttgccctgc
     3121 agtacatcgc tttccgacaa ttcgatatcc gatgccgaga cgctgcgctt gtcggcggcc
     3181 ttgaggcgtt cgttgtattt caacaccctg tcgtagtttg cctcggcttt tgccagttca
     3241 gctttccgca gggccactgt cgccctgtac tccctgcggt cgagagtaaa aagaagatcg
     3301 ccggccttga ccatcgaacc ttccttgaaa tgaacctttt ctatctctcc actgacctgg
     3361 ggtttcagcg aaaccgcctg gatgggttcc accctgccca cgtattccct gcctacggag
     3421 agatcggttt tttctaccgt atacaggacg accgccggag cctgcggctg cattgggacc
     3481 tgctggccgt ccgacctgcc gctgagatac gatcttccga aaaaaacgat gaccgctatg
     3541 agcagtatcc cgatgatcgt ttttaccatc ctgaaattgg gtttgcttcc gcttcctgca
     3601 tttttgtact gttccgaaga tgtcattgtg caatacacct accctttctg ttttgtattg
     3661 tttgccgaat cgagctcttt cttgaaggcg tcgaatatga aatcaatatg ttccgttaag
     3721 tcgacgtgat aaaaatcatg cacctggaac acgaagatgc cctgaaacat ggcggtaacc
     3781 atcagagaca ggtctctggg tgaaacatcc atcctgatgg ttccttcttc ctgagccctg
     3841 acgagcagcc tttccgtcat cttctgttcc agatccaggc actccagtat catttcaaga
     3901 acttttttcc ggatctcttc aggccattcc tctccgcggt gcatcagcct gtgaattttc
     3961 ttgaattctt cgtcctgaag aggtttttgc atcttctttt tatagtatgt acgcatgtcg
     4021 tcgatggatt caagagacgc gctatcagcg caaaattcct ctgccatctg ggtacggata
     4081 tttaggatca cgtgaagcag gacgtcattc ttgtttttga aatgccagta tatggcccct
     4141 ttcgagagac cgattttcct tgctatttca gtcatggaaa cgcttgagaa cggtttttcg
     4201 ctcatgatct ccagagcggc ttccagaagc tgtttccttg tctccagagc ttcttccctg
     4261 acccgtcgca ccattagctc acattctcct cccccaaaga aaacgaaacc tcgagattat
     4321 acataccttc atgcatgtat gtcaatccaa agcctatccc ggaaagcaat tgcgctgcga
     4381 gatgaactcc ccggttatgc tatgctttac tcatcgtgat gcaaaacaaa aaaactggcg
     4441 ggtgatcttt aatggctttc aagggcgaac ttgattccgt gaaaatagtt acggtcctcg
     4501 acgattatgc aggatatgaa acatccttcc ttgcccagca cgggatagcg ctgcttctcg
     4561 aggcctgctc taacggggtc tgcaaaagga tcctcatgga cgcggggcaa tcggctctgc
     4621 cgatccttca caacatgaaa ctcatggaca tagatcccgc ctcgatcgat gccgtcataa
     4681 tttcccactg ccactatgac cac
//
LOCUS       DAOTIL010000095         4666 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminobacteriaceae bacterium isolate AMR_MDS_3026
            NODE_21854_length_4666_cov_4.380828, whole genome shotgun sequence.
ACCESSION   DAOTIL010000095 DAOTIL010000000
VERSION     DAOTIL010000095.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868050
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminobacteriaceae bacterium (wastewater metagenome)
  ORGANISM  Aminobacteriaceae bacterium
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae.
REFERENCE   1  (bases 1 to 4666)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4666)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:19:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,224
            CDSs (total)                      :: 3,198
            Genes (coding)                    :: 3,184
            CDSs (with protein)               :: 3,184
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4666
                     /organism="Aminobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21854_length_4666_cov_4.380828"
                     /isolate="AMR_MDS_3026"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:3034609"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..810)
                     /locus_tag="P5201_03085"
     CDS             complement(<1..810)
                     /locus_tag="P5201_03085"
                     /inference="COORDINATES: protein motif:HMM:NF015644.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0182 family protein"
                     /protein_id="HRV97548.1"
                     /translation="MFCMMSKNDWFPDNEWFPGDDSSSPKRPVFRLPKVNKGLLLLGG
                     AVLLVMVLFPALAEFYTDWLWYDTEGYGSVFLTRILARLPLFAAGFLLFASAIWVNLA
                     FVRRNLLALHPEFTPLLASKAAGWAIGAVALFLGFSNGVAASTEWAMVLRFFNATPFG
                     TADPIFGNDTGFYVFALPFWTFLHTRLLNILFLCVVTAAAAYGAIILPRNRDFSMITI
                     PQSVRTHLTLLASAGAFLWGVGYLLDRYGILFSPRGVTFGAGYTDVHAELFA"
     gene            1091..2083
                     /gene="dctP"
                     /locus_tag="P5201_03090"
     CDS             1091..2083
                     /gene="dctP"
                     /locus_tag="P5201_03090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009164337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter substrate-binding protein DctP"
                     /protein_id="HRV97549.1"
                     /translation="MFKKSFATTAVMLLALSLFCTAAFAKVEIKLSNQLPPSHHISKG
                     LVVFSEKVAEYSGGDVTVRIFDSAQLFKDTEIVEALQDGLVEAGLVPVNKWSGMIPAA
                     DIFEMPFVFKDFESPKKFLNSGADKLLDGEFQKKGVKNLFWVDYGYIQFFNNKRPLKS
                     PEDFKGLTMRTFSSGDAETLRALGAAPTVMSSSEMYMALQRGTVEGATTGMPAAVSRK
                     IHEVQKYMTMANYTTAQFVVQANLGWWEKLSQAEKDVILKAGLDAEAWIRQAIEASEN
                     EAEKVIRDAGVEIHVLSAEERPLFIEATDPVRKAFAEKTGEIGKTLIDMVMAID"
     gene            2189..2713
                     /locus_tag="P5201_03095"
     CDS             2189..2713
                     /locus_tag="P5201_03095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014162389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0031317 - tripartite ATP-independent
                     periplasmic transporter complex [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015740 - C4-dicarboxylate transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter small permease"
                     /protein_id="HRV97550.1"
                     /translation="MIRHIRSAVEKGNLILGYVSGLGILLMGLILFYEVVMRYFFRAP
                     TIWAQETAVYIFMWTMLAGGAYTLQKGKHVRIDLIIEHLSIRTQKILEMITSFAGMFF
                     CAVVTWQAYEMIAASIGYEKVSATPLRVPMWIPQMALLLGFALLTFQFAIIIADRYAE
                     VFGKGAAGEEKKTC"
     gene            2707..3987
                     /locus_tag="P5201_03100"
     CDS             2707..3987
                     /locus_tag="P5201_03100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014162390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0031317 - tripartite ATP-independent
                     periplasmic transporter complex [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter large permease"
                     /protein_id="HRV97551.1"
                     /translation="MLSFSFVVVLLIGILFMGLPVAFSLGGTSLVLIWLFELPMKVVG
                     ATIFTSLEGFVLLAIPLFVLMSQILLDGRIGDDLFDVMNAWVRHLPGGLAIATILACA
                     FFAAITGSGAATAATIGMVAYPAMTERGYDKRFTLGLLAAGGTLGILIPPSIPLILYG
                     AITEESVGKLFIAGVVPGLVLTALFIVYAVYKSKRGGFTPMERTSWSERLRVTRKNIW
                     GIFLPFLIIGGIYSGAFTPTEAAAVGLVYSLFITLFVYRTLSFRDIPAICLRSVTTSC
                     MVAIIIAGAILFGRVMTLLRIPQILTELVISNNFSVLMFIIAMNVLMMILGCILETVS
                     IILLTMPLVTPILHALGIDPIWYGIVVTVNMTLALITPPVGMNLYVINGLRSDIRMGE
                     VIQGVTPFIVIMALFLIGVIAFPAMSTWLPSIMQ"
     gene            4001..>4666
                     /locus_tag="P5201_03105"
     CDS             4001..>4666
                     /locus_tag="P5201_03105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009202398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isocitrate lyase/phosphoenolpyruvate mutase
                     family protein"
                     /protein_id="HRV97552.1"
                     /translation="MEKRGAQLRKLLARREILVAPGAYDGLSARLVESAGFEAVYVTG
                     YGLSASVLGKPDTGLLTLTEMASRVSAIAETVSVPVIADGDTGYGNAVNVARTVREYE
                     KAGAACIQLEDQVAPKKCGHMLGREVIPAAEMAGKIRAACDARRDDDFLIMARTDART
                     THGIDEADVFALHQEGGQTCIQVFFFRTGQNWGNRAYFPRTGASLEPRKILGAFIAQF
                     YDNK"
BASE COUNT          975 a         1319 c         1431 g          941 t
ORIGIN      
        1 cgcgaagagc tccgcgtgga cgtccgtgta ccccgcgccg aaggtgacgc cgcgcggaga
       61 gaaaagaatg ccgtagcggt cgagcaggta gccgacgccc cagagaaacg ctccggcgga
      121 tgcgagcagc gtcaggtgcg tccgcacgct ctgcgggatg gttatcatcg agaagtcgcg
      181 gtttcgcggc aggatgatcg cgccgtaggc ggccgcagcg gtgactacgc agaggaagag
      241 gatattcagc agccgcgtgt gaagaaacgt ccagaagggc agggcgaaga cgtaaaagcc
      301 cgtgtcgttg ccgaagatgg gatccgccgt gccgaaaggc gttgcgttga agaaacgcag
      361 caccatcgcc cactccgtgg aggcggcgac gccgttggaa aagcccagga agagcgcgac
      421 ggctccgatg gcccacccgg ccgctttcga cgcgagaagc ggtgtgaatt cgggatggag
      481 agccagcaga ttccgccgga cgaaggccag gttgacccag atggccgagg cgaagaggag
      541 gaagcccgca gcgaacaggg gaagcctggc gaggatgcgg gtcagaaaga cggacccgta
      601 cccttcggtg tcgtaccaca gccagtcggt ataaaattcc gccagggcgg ggaacagcac
      661 cattacaagg agaacggcgc ctccaagcag caggagacct ttgttcacct tcggaagccg
      721 gaagaccgga cgcttgggag acgatgaatc gtcgcccggg aaccactcgt tgtcgggaaa
      781 ccagtcgttt ttactcatca tacaaaacat tgtaacggac tttctacctt tgtcaaagag
      841 tgaaaaacag cgcagcgctg gaggatatgc catccgttga tgtcgaaaga gaaatatgcc
      901 gcgccgagtt cttttatttc aaagggtgga attgaaggtg ggggagtaat ttgaaaatac
      961 cgctccggag cgatttatct tgacagagcg ccgatgcgta tggtaacaag agtaatccgc
     1021 gtatcaacac ggggcacgtt atttgttcag gaaagtttca acacatttcg gtaaaggagg
     1081 aaatcaaagt atgttcaaaa agtcatttgc aacaacggca gttatgctcc tcgcgctctc
     1141 cctgttctgc accgccgcgt tcgccaaggt ggagatcaag ctttccaacc agctcccccc
     1201 ctcgcatcat atctcgaagg ggctcgtcgt tttttccgaa aaggtggcgg agtattccgg
     1261 aggagacgtt acggtccgga tcttcgactc ggcccagctc ttcaaggata cggaaatagt
     1321 ggaggcgctt caggacggtc tcgttgaagc gggtttggtg ccggtcaaca agtggtccgg
     1381 catgatcccc gcggcggaca tcttcgagat gcccttcgtc ttcaaggatt tcgagtcccc
     1441 caagaaattc cttaactctg gcgcggacaa gctccttgac ggcgagttcc agaagaaggg
     1501 cgtcaaaaac ctcttctggg ttgactacgg gtacattcag ttcttcaaca ataagcgtcc
     1561 cctgaagtcc cccgaggact tcaagggcct tacaatgaga accttcagca gcggcgacgc
     1621 ggaaacgctg cgcgcgctgg gcgccgcgcc caccgtcatg agttcgtccg agatgtatat
     1681 ggcgctgcag agaggcaccg tagaaggcgc aacgaccggt atgccggccg ccgtctcccg
     1741 caaaatccac gaagtgcaga agtacatgac gatggcgaac tacactacgg cgcagttcgt
     1801 ggtgcaggcg aacctcggct ggtgggagaa actctcccag gccgagaagg atgtgatcct
     1861 gaaggccggt cttgacgccg aggcgtggat tcgccaggcc atcgaggcgt ccgagaacga
     1921 agccgaaaaa gtgatccgcg acgccggagt ggagatccac gtactgagcg ccgaggagag
     1981 gccgctcttc atcgaggcca cggatcctgt gcgcaaggcc ttcgccgaaa aaacgggaga
     2041 gataggcaaa accctgatcg acatggttat ggcgatcgac taggtttttc gggagaaaga
     2101 gtcctcacgg gagttgtctc gaaagagcgg gaaagggact ctctttcccg ctctttacta
     2161 taaattatga ataaaggggc atgcgtagat gattcgacat attcgcagtg cggtggagaa
     2221 aggaaacctg atcctgggct acgtcagcgg ccttgggatt cttcttatgg ggctgattct
     2281 gttttacgag gttgttatgc gctatttctt cagggcgccc accatctggg cccaggagac
     2341 cgctgtgtac atcttcatgt ggacgatgct cgccggagga gcctacacgc tccagaaggg
     2401 gaagcacgtg cgcattgacc tcattatcga gcacctctcg atacggacgc agaagatcct
     2461 cgaaatgatc accagcttcg ccggaatgtt cttctgcgcg gtggtcacgt ggcaggcgta
     2521 cgagatgatc gccgcgtcca tagggtatga aaaagtatcg gcgacgccgc tgcgggtgcc
     2581 gatgtggatt ccgcagatgg cgctgctgct cggtttcgct ctgttgacat tccagtttgc
     2641 gatcattatc gccgaccgct acgcggaagt attcggcaag ggcgccgccg gagaggagaa
     2701 aaaaacatgc tgagcttttc cttcgtcgtc gtgctgctca tcggaattct cttcatggga
     2761 cttccggtgg ccttttctct cggtggaacg tcgcttgtgc tgatctggct tttcgagctt
     2821 cccatgaagg tcgtgggcgc gaccatattc acatcgctcg aaggctttgt gctgctcgcc
     2881 attccgctct tcgtgctgat gagccagata ctgctcgacg gacggatcgg cgacgacctt
     2941 ttcgatgtca tgaacgcgtg ggtgcggcac cttcccggcg ggcttgccat cgcgacgatt
     3001 cttgcgtgcg ctttcttcgc cgccatcacc ggctccggcg cggcaaccgc ggcgacgatc
     3061 gggatggtgg cctacccggc gatgacggag cgcggatacg acaagcgatt cactctcggt
     3121 ctgctggccg cgggcggcac gctcggcatc ctcattccgc cgagcattcc ccttatcctc
     3181 tacggcgcga ttaccgagga gtccgtcgga aagctcttca tcgcgggggt tgtccccggt
     3241 ctcgtcctga cggcgctctt tatcgtgtac gcagtctaca agagcaagcg gggcggtttt
     3301 acccccatgg agcgaacctc ctggagcgag cgcctcaggg ttacacggaa gaacatctgg
     3361 ggtatcttcc tccccttcct cattatcgga ggtatttatt ccggcgcatt cactccgacc
     3421 gaggccgcag ccgtggggct tgtctacagc cttttcatca cgctcttcgt ctacaggacg
     3481 ctgagtttcc gcgacatccc ggcgatctgc ctccgctccg tcactacgtc gtgcatggtg
     3541 gcgatcatca tcgccggcgc cattctcttc ggacgcgtga tgacgctgct gcggattccg
     3601 cagatcctca ccgaactcgt catcagcaac aacttctccg tgctgatgtt catcatcgcg
     3661 atgaacgtgc tgatgatgat cctcggctgc atactggaga cggtctccat catcctgctt
     3721 accatgccat tggtgacccc gatactccac gcgctcggta tcgacccgat ctggtacggt
     3781 atcgtggtca cggtgaacat gacattggcg ctgatcacgc ctccagtggg tatgaacctc
     3841 tatgtcatca acggactgag gtcggatata cggatgggag aggtcatcca gggcgtcacc
     3901 ccgttcatcg tcatcatggc gctcttcctg atcggagtca tcgccttccc ggcgatgagc
     3961 acgtggctgc cgtcgatcat gcagtaacgg acggatgatc atggaaaaaa gaggagcgca
     4021 gctacgcaag ctcctggcgc ggcgggagat tctcgtcgcg ccgggagctt acgacggtct
     4081 ttccgcccgg ctcgtcgagt ccgccgggtt cgaggccgtc tacgtgacgg ggtacggtct
     4141 ctctgcaagc gtgctgggaa agcccgatac cggactgctc acccttacgg agatggcctc
     4201 gagagtgtcg gcgatcgcgg agacggtttc cgttccggtc atcgccgacg gcgacacggg
     4261 gtacggcaac gcggtcaacg tggcgcggac tgtccgcgag tacgagaagg ccggggcggc
     4321 ctgcattcag ctggaggatc aggttgctcc gaagaagtgc gggcacatgc tgggacgcga
     4381 agtgatccct gcggcggaga tggccggcaa gatcagggcg gcctgcgacg cccggaggga
     4441 cgacgacttc ctcatcatgg cgcggacgga tgcgaggacg acgcacggca tcgacgaggc
     4501 ggacgtcttc gcgctgcacc aggaaggcgg gcagacctgc attcaggtgt tcttcttccg
     4561 caccggccag aactggggca accgcgccta tttcccgcgg accggcgctt cgctcgaacc
     4621 gcgcaagatc ctcggtgcct tcatcgccca gttctacgac aacaag
//
LOCUS       DAOTIL010000096         4647 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminobacteriaceae bacterium isolate AMR_MDS_3026
            NODE_21975_length_4647_cov_3.653746, whole genome shotgun sequence.
ACCESSION   DAOTIL010000096 DAOTIL010000000
VERSION     DAOTIL010000096.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868050
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminobacteriaceae bacterium (wastewater metagenome)
  ORGANISM  Aminobacteriaceae bacterium
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae.
REFERENCE   1  (bases 1 to 4647)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4647)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:19:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,224
            CDSs (total)                      :: 3,198
            Genes (coding)                    :: 3,184
            CDSs (with protein)               :: 3,184
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4647
                     /organism="Aminobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21975_length_4647_cov_3.653746"
                     /isolate="AMR_MDS_3026"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:3034609"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..315
                     /locus_tag="P5201_03110"
     CDS             <1..315
                     /locus_tag="P5201_03110"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRV97553.1"
                     /translation="LVKSASAAGTDEQAIVQYFHNPASDKRIVIFGHTHESRVIPSQT
                     HDGKSAVYVNSGTWIDKNNNLPTMTFVVIAPQGSSRHAGLYHYSHEGAITTLDTLKLE
                     GF"
     gene            complement(406..759)
                     /locus_tag="P5201_03115"
     CDS             complement(406..759)
                     /locus_tag="P5201_03115"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009202147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004521 - endoribonuclease activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system PemK/MazF family
                     toxin"
                     /protein_id="HRV97554.1"
                     /translation="MTLLKRGMVIDVNLSPTQGSETGKIRPCVVVTNDVYNAKVPVVQ
                     VVPVTEWNEKKGHIMTNVELLPSPENGLTKKSVADCLQTRPVDHRFRMVGVRGALTSE
                     EMHTIDDALRMVFSL"
     gene            complement(756..989)
                     /locus_tag="P5201_03120"
     CDS             complement(756..989)
                     /locus_tag="P5201_03120"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRV97555.1"
                     /translation="MLQTKISLTPSLAEFLDDHRSYGFKDKSLMVRSALLRFKEELEL
                     ESLARSADLYAELYDQDEESRELTESALQGWPE"
     gene            1257..2279
                     /locus_tag="P5201_03125"
     CDS             1257..2279
                     /locus_tag="P5201_03125"
                     /inference="COORDINATES: protein motif:HMM:NF014647.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BMP family protein"
                     /protein_id="HRV97556.1"
                     /translation="MKKVLALTLCFALVFGLTLTVAGQNAASAAEVVKIALIIESTVD
                     DKGWCQAMHDGILQAQKELPGRIEYSYSEKMKPVDAGSAVLQYVSQGYHIVIGHGAQY
                     KNLMLEMAEEYPEVTFAFGTSAEIGPENVFTYMPQSEETGYLSGLIAGMTTKKNIVGL
                     VGPVDGGDAARYNRGFVLGVHAVNPGAKIMVAHTGSFADFVKAGEVAETQIKAGADVL
                     TGSSQQALGALRAVADHKDAPIWWVGQDIAQIHITEGYKCIAASSYNYAAVIVGLVAK
                     LDAGVRGGECIPMNFSNNGFVFAFNETLKDMYTGAIEQKVNEALEKFRATPETLNWSA
                     VDYSKL"
     gene            2347..3885
                     /locus_tag="P5201_03130"
     CDS             2347..3885
                     /locus_tag="P5201_03130"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HRV97557.1"
                     /translation="MEPNTRKITSLRVEHITKRFPGVLACNDISLSVAEGEVLALVGE
                     NGAGKTTLMNILMGLYRPDEGRILINGEEVVFRSPNDAFAAGLGMVHQQYMLVANMTV
                     LENIALGFTQAWSPLKLNLDMVRARIDEVAAKYGLAVDPDAFVWQLSVGEQQRVELVK
                     TLCLGARFLILDEPTSALTPQETDELILLLKRMTSELSIVFISHKLHEVKNLSDKVTI
                     LRHGSVVFEGSTADHSSGDIAALMTGREIHLPRNEAPTCEGNPALEIRGLKVRSDRGF
                     LAVDGLDLSVCAGEIVGLAGVSGNGQRELAEAINGLRKVEGGEILLFGQELANRSPRA
                     VIDAGMGYIPEERNTEGIVPSFSMKENFILKDADEAQFSARSFLRLKEIDRNAEALRT
                     EFDIRSPDTKVAAGSLSGGNIQKVILAREISRKPRFLIAVYPTRGLDMGAAEFIHKRL
                     LEKRLEGIGILLISEELEEILDLSDRVAVIFKGRIIDTLDRRSADSRKIGILMAGVHN
                     DEAV"
     gene            3869..>4647
                     /locus_tag="P5201_03135"
     CDS             3869..>4647
                     /locus_tag="P5201_03135"
                     /inference="COORDINATES: protein motif:HMM:NF014684.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HRV97558.1"
                     /translation="MTKQFKALYKTVVILLAAAAAMLLGALILWTIGADVTKTYSVII
                     LEPLKNKIQIGEVLIRAIPLTIIALGISVAYRSGIINIGAEGQMAMGILAATAVALAL
                     PGLPKYALLPLAMIAGTLGGAAWGFIPGILKAKLHVSELLSTVMLNYIAAQFYTFCLR
                     GPMLDPAEITMGSGTPQSMRLTKAIWLDRLVPGTRIHTGVYIALAMAFIIWLLLWRTS
                     WGYKMRAAGASERAARYGGIGVAGYLVVAMMISGAFAGLAGA"
BASE COUNT          943 a         1510 c         1373 g          821 t
ORIGIN      
        1 ctcgtcaagt cggcctccgc ggcgggaacg gacgagcagg cgatcgtgca gtacttccac
       61 aaccccgcgt ccgacaagcg gatcgtcatc ttcgggcata cccacgaatc cagagtaatc
      121 ccctcgcaga cgcacgacgg gaagagcgcc gtgtacgtga actccggcac gtggatcgac
      181 aagaacaaca acttgccgac aatgacgttc gtcgtgatcg ccccgcaggg ctcctcgcgg
      241 catgcgggcc tctaccacta ctcgcacgag ggcgccatca cgacgctgga tactctgaag
      301 ctcgaaggct tctaacgaaa ccggccggaa cagaaaacag aaagagagga agacgcgaaa
      361 tgcgcctttc tctctttttt cttattctcg catgtccgga gcgaattaga gtgaaaaaac
      421 cattcgcaat gcgtcgtcta tcgtgtgcat ctcttcggag gtcaacgcac cccgtacccc
      481 gaccatacgg aaccggtgat ccacgggacg ggtctgcaaa cagtccgcca ccgacttttt
      541 cgtcaggccg ttttcaggcg aaggaagcag ctcgacattc gtcatgatat gccctttttt
      601 ctcgttccac tcggtgaccg gcacaacctg caccacaggg accttcgcat tgtacacatc
      661 gttcgtcacg acgacgcacg ggcgaatctt ccccgtctcc gatccctgcg tcggggaaag
      721 attcacatcg atcaccatgc cccgcttcag caaggtcatt ccggccaccc ctgcagcgcg
      781 ctctccgtga gctcgcggga ctcctcatcc tgatcataga gctccgcata caggtcggcg
      841 gaacgcgcca ggctttccag ttcaagctct tctttaaaac gaagcagcgc gctgcgcacc
      901 atcaagcttt tatctttgaa tccataggag cgatgatcgt ccagaaactc cgccaatgac
      961 ggtgtcaggc tgatttttgt ttgcagcatc tcaacacctc cccaaagcaa gcagaattta
     1021 ttcagcccca attataggtc ctaatttcaa gaccgtcaac aagaccccat ccagtggacc
     1081 acggaggcga agtaaatgct ccgcgagcgc ggtcggcacg ctcttcgtct ttccatacac
     1141 acgagctcgt tccgcgtatg gagcgccgtt cggcgcttga caatcttttg aaacccctta
     1201 cactggatgc acatttccgc agcttttaaa aaaacgaaat tcaaggaggg gtctgtatga
     1261 agaaagtatt ggctctcacg ctctgttttg ctcttgtttt cgggttgacg ctcaccgtcg
     1321 cgggacagaa cgccgcgtcc gccgccgagg tcgtgaagat cgcgctcatc atcgagagca
     1381 ccgtcgacga caaggggtgg tgccaggcga tgcacgacgg cattctccag gcgcagaaag
     1441 agcttccggg acgcatcgag tacagctaca gcgagaagat gaagccggtc gacgccggtt
     1501 ccgcagttct tcagtacgtc tcccaggggt accacatcgt catcggacac ggcgcgcagt
     1561 acaagaacct gatgctcgaa atggccgagg agtacccgga ggtcaccttc gccttcggca
     1621 ccagcgcgga gataggcccc gagaacgtct tcacctacat gccccagagc gaggagaccg
     1681 gctatctctc cggcctgatc gcaggcatga cgactaaaaa gaatatcgtc gggctcgtcg
     1741 gcccggtcga cggcggcgac gcggcgcgct acaaccgcgg cttcgtcctc ggcgtgcatg
     1801 cggtcaaccc cggggcgaag atcatggtcg cccacaccgg ctcgttcgcg gacttcgtga
     1861 aggccggaga ggttgcggaa acccagatca aggcgggcgc ggacgtgctc accggctctt
     1921 cgcagcaggc cctcggcgct ctgcgcgccg tcgcggacca caaggacgcc cccatctggt
     1981 gggtcggcca ggacatcgcc cagatccaca tcaccgaggg gtacaagtgc atcgcggcgt
     2041 cctcctacaa ctacgcggcg gtcatcgtcg gccttgtcgc caagcttgac gccggagtac
     2101 ggggcggcga gtgcatcccg atgaacttct ccaacaacgg gttcgtcttc gccttcaacg
     2161 aaacgctgaa ggacatgtac acgggcgcga tcgagcagaa ggtcaacgag gccctcgaaa
     2221 agttccgggc aaccccggag acgctcaact ggagcgccgt cgactactcc aagctgtaat
     2281 ccgcatgtat gtcctgcggg ggcgccccct ccgcaggaca tttttttctc tcgggaggtc
     2341 tcccgcatgg aaccgaatac ccgcaaaata accagtcttc gagtggaaca catcaccaag
     2401 cgcttccccg gagtgctcgc ctgcaacgac atctcgctct ccgtggccga gggcgaagtg
     2461 cttgccctcg tcggagagaa cggcgccgga aagacgacgc tgatgaacat cctgatgggg
     2521 ctctaccgcc ccgacgaggg acgcatcctg atcaacgggg aggaagtcgt cttccgctcc
     2581 cccaacgacg cattcgccgc cggacttggg atggtgcacc agcagtacat gctggtggcg
     2641 aacatgacgg tgctggagaa catcgccctg ggcttcacgc aggcgtggtc tcccctgaag
     2701 ctgaacctcg acatggtgcg cgcccgcatc gacgaggtgg cggcgaagta cgggctcgcc
     2761 gtcgacccgg acgccttcgt ctggcagctc tccgtcggcg aacagcagcg cgtggagctg
     2821 gtgaagacgc tctgcctcgg cgcacgcttc ctcatcctcg acgaaccgac cagcgccctc
     2881 acgccgcagg agacggacga gctgatcctc ctgctcaaac ggatgacgag cgaactctcc
     2941 atcgtcttca tcagccataa gctccacgag gtcaagaacc tctcggacaa ggtcaccatc
     3001 ctccggcacg gctcggtcgt cttcgagggc agcaccgccg accactcctc gggcgacatc
     3061 gcggcgctga tgacaggacg cgagattcac ctccccagga acgaggcgcc gacgtgcgag
     3121 ggcaatccgg ctctcgaaat ccgcgggctc aaggtcagga gcgaccgcgg cttcctcgcc
     3181 gtcgacgggc tcgacctctc ggtctgcgcg ggcgagatcg tcggactcgc cggggtctcc
     3241 ggcaacgggc agcgcgagct ggccgaggcg atcaacggcc tgcgaaaagt cgaggggggc
     3301 gaaatcctcc tcttcggaca ggagcttgcg aaccgctctc cgcgcgccgt gatcgacgcg
     3361 gggatggggt acattcccga ggagcgcaac accgagggaa tcgttccctc cttctccatg
     3421 aaggagaact tcatcctgaa ggacgccgac gaagcgcagt tttcggcgcg ctcgttcctc
     3481 aggctcaagg agatcgaccg gaacgccgaa gcgctgcgaa cggagttcga catccgcagc
     3541 cccgacacga aggtcgcggc gggctcgctc tcgggaggga atatccagaa ggtcatcctc
     3601 gcgcgggaga tctcccgcaa accccgcttc ctcatcgcgg tctaccccac gcgcggcctc
     3661 gacatggggg ctgcggagtt catccacaag cgcctcctgg agaagcgcct ggaggggatc
     3721 ggcatcctgc tcatcagcga ggagctggag gagatcctcg acctctccga ccgcgtcgcc
     3781 gtcatcttca aggggcgcat catcgacacg ctcgacagac ggtcggcgga cagcagaaaa
     3841 atcggcatcc tgatggcagg tgtgcacaat gacgaagcag tttaaggcgc tctacaagac
     3901 cgtcgtcatc ctcctcgcgg cggccgcggc gatgcttttg ggcgcgctca tcctctggac
     3961 catcggcgcg gacgtgacga agacctactc ggtcatcatc ctcgaaccgc tcaagaacaa
     4021 aattcagatc ggcgaagtgc tcatccgggc catcccgctg acgatcatcg cgctgggaat
     4081 ctcggtcgcc taccggagcg gcatcatcaa catcggcgcc gaggggcaga tggcgatggg
     4141 catcctcgcc gcgacggccg tggcgctcgc cctccccggc ctgccgaagt acgcgctgct
     4201 cccgctggca atgatcgcgg gaacgctcgg cggggcggcc tggggcttca ttccgggcat
     4261 cctcaaggcg aagctccacg tcagcgagct cctgtcgacg gtcatgctga actacatcgc
     4321 cgcgcagttc tacaccttct gcctgcgcgg gcctatgctc gaccccgccg agatcacgat
     4381 ggggagcggc acgccgcagt cgatgcgcct gacgaaagct atctggctcg acaggctggt
     4441 ccccggcacg cgcatccaca ccggggtcta catcgcgctg gcgatggcct tcatcatctg
     4501 gctgctgctc tggaggacgt cgtgggggta caagatgcgc gccgccggag cgagcgaacg
     4561 ggcggccagg tacggcggca tcggcgtggc ggggtatctc gtcgtggcca tgatgatcag
     4621 cggtgcgttc gccggactcg cgggagc
//
LOCUS       DAOTIL010000097         4594 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminobacteriaceae bacterium isolate AMR_MDS_3026
            NODE_22323_length_4594_cov_3.367482, whole genome shotgun sequence.
ACCESSION   DAOTIL010000097 DAOTIL010000000
VERSION     DAOTIL010000097.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868050
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminobacteriaceae bacterium (wastewater metagenome)
  ORGANISM  Aminobacteriaceae bacterium
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae.
REFERENCE   1  (bases 1 to 4594)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4594)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:19:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,224
            CDSs (total)                      :: 3,198
            Genes (coding)                    :: 3,184
            CDSs (with protein)               :: 3,184
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4594
                     /organism="Aminobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22323_length_4594_cov_3.367482"
                     /isolate="AMR_MDS_3026"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:3034609"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1049)
                     /locus_tag="P5201_03140"
     CDS             complement(<1..1049)
                     /locus_tag="P5201_03140"
                     /EC_number="6.3.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013048854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003883 - CTP synthase activity [Evidence
                     IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0006241 - CTP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CTP synthase"
                     /protein_id="HRV97559.1"
                     /translation="MAKYVFVTGGVVSSLGKGITAASLGVLLKKRGLRVSIIKLDPYL
                     NVDAGTMNPFQHGEVFVTDDGAETDLDLGHYERFIDESLSANNNVTTGKIYSSVIAKE
                     RRGDYLGGTVQVIPHITNEIQERILRAGEKSDIVIAEIGGTVGDIEGQPFLEAIRQMS
                     SRVGRENVLYCHVTLVPWLEAARELKTKPTQHSVQELRRIGILPQILVCRSHYAIEEE
                     MRDKMALFCNVQPEAVIQALDEPTIYKVPLSLYEQKFDLLVLKGLSLESPREPDLSDW
                     RRVVDGFCSPAGAVDIVMVGKYINHKDAYLSVVEALHHAGIHHDVKVNIRSVEAEEIE
                     EHGSEKILSGAHGILV"
     gene            complement(1185..3173)
                     /locus_tag="P5201_03145"
     CDS             complement(1185..3173)
                     /locus_tag="P5201_03145"
                     /inference="COORDINATES: protein motif:HMM:NF013700.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing M23
                     family metallopeptidase"
                     /protein_id="HRV97560.1"
                     /translation="MKKRNSPSPFEVRNKKAPRKKGAGFLPFAESPKGVRLGRISCFL
                     VIIVMLATAAFLTLSTADRTVGAAWGNLSDSKDSGEFILPSSGFIVVEMSNVLLDEDG
                     YELGMETDSLFALRGRRGEPGLLPLGNEVPASAKKTKDVAENSVQKLDVDTSQPKEVS
                     SGSEASLPMTDFSWSISEQFSRGFASSSMTLGEEESASSEGAFRFPDRLVEREGQLGG
                     NSLFTTFGVSRMTEFHPRLSHGFVPGESSFSTANGALFADSAVSRLVSFKASSENRGE
                     SSSSDVSDASDFEAVVLSVGDDGKKVPLEAGEDAEEAEDVTLAQVSVLWKEHIVKAGQ
                     TLSDIAVAHGVAVSDIVKANELKNANRLSEKQLLLIPNNSAAVEATLEEVLTRKARLV
                     AAREKVLPIKITAYVVAEGDSLWSIANSQNIEIDTLFGCNDLRNPNVIRPGATLRIPN
                     QDGIFHKIKSGETLAGVAKKYEIAADRIRQANGEAALNPFPVGKEIFLPGARPEAVES
                     SPSSSSSSRSAARSSKSAPAKVSSSSSRSYRWPVVGKINSPFGWRRHPVTRRRDFHTG
                     IDIKAPRGRTIQAARGGRVEYSGWMGGYGRVVVIDHGDGHSTLYAHCSSLAVRKGNKV
                     SQGQAIATVGTTGRTTGPHLHFEIRRGNNPVNPLGMLR"
     gene            complement(3259..4488)
                     /gene="rho"
                     /locus_tag="P5201_03150"
     CDS             complement(3259..4488)
                     /gene="rho"
                     /locus_tag="P5201_03150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005658845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0008186 - ATP-dependent activity, acting
                     on RNA [Evidence IEA];
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor Rho"
                     /protein_id="HRV97561.1"
                     /translation="MAALLNPDLRKIAKELGIVGFSSLRKDDLVIAILKAQAEELNYR
                     FGGGTLEILQEGYGFLRPKGLLPSDHDVYVSASQVRRFGLRNGDVIWGLVRPPRDSEH
                     YEALLRVEMVNFSDPEAARKRPHFGAITPIFPDEKLSLETDPKEISTRLVDLFAPIGK
                     GQRALLVSPPKAGKTTLLKKIANAVTVNYPDIILMVLLIDERPEEVTDMARSVDGEII
                     ASTFDRPADEHLRVANLALEKAKRLVEVGKDVVLLLDSITRLARASNLVVPPSGRTLS
                     GGMDPAALYFPKRFFGAARNIEEGGSLTIIGTALVDTGSRMDEVIYEEFKGTGNMELH
                     LSRKLSEQRIFPAIDIVKSGTRREELLFTEDELSRLWLLRRRIVNVDESGALNLIIDK
                     LKQSSTNKEFLLTIKKG"
BASE COUNT          984 a         1372 c         1209 g         1029 t
ORIGIN      
        1 accagaatac catgcgcgcc tgaaaggatc ttctcgcttc catgctcttc gatctcttct
       61 gcttccactg aacggatatt caccttcacg tcatggtgaa tcccggcgtg atggagcgct
      121 tccaccacgc tcaggtaggc gtccttgtga ttgatatatt tccccaccat gacaatatcg
      181 actgctcccg cgggggagca gaatccgtcg acgactcttc tccagtcgga aagatccggt
      241 tcgcggggag attcaagaga aaggcccttg agtacgagca agtcgaactt ttgttcataa
      301 aggctgagag gcaccttgta gatcgtcggt tcgtcaagag cctgaatgac tgcttccggc
      361 tggacgttgc agaagagcgc catcttgtcg cgcatctctt cctcgatagc gtaatgggaa
      421 cggcacacca ggatttgagg gaggatacca atacgccgca gctcctgaac gctatgctgc
      481 gtcggtttgg tcttcaattc gcgcgccgcc tcaagccagg gcacaagcgt cacgtgacaa
      541 tagagcacat tctcgcgtcc taccctggac gacatctgac ggatggcctc caggaagggc
      601 tgtccttcaa tatcgcccac ggttccgccg atctccgcaa tgacaatgtc gctcttctcg
      661 cccgcccgga ggatacgctc ctgaatctcg ttggtgatgt gcggaatgac ctgcaccgtc
      721 ccccccagat aatcgccgcg gcgctccttg gcgatgacgg aagagtagat cttccccgtt
      781 gtcacattgt tgttcgcgct cagcgactca tcgataaacc gttcgtaatg tcccagatcc
      841 aggtcggttt cggcgccgtc gtcggtcacg aaaacctctc cgtgctggaa gggattcatc
      901 gtccccgcgt ccacgttcaa atacggatca agtttgatga tggagacgcg cagccccctc
      961 tttttgagca acacgcccag cgacgccgca gtgataccct ttcccaacga cgacaccact
     1021 ccgcctgtga caaacacgta cttcgccatt tccctcatcc tccactagta ttcagaaaaa
     1081 atcgttcacg agacgaacac gtcaacgcat caaaaaaaca tggaccaggg agaaccccgg
     1141 tccagaatat cgaaagagaa tccatccgtc atggaggata ttgcctatct taacatcccc
     1201 agcgggttca ccggattgtt tcccctgcgg atttcgaagt gcaggtgcgg accggttgtc
     1261 cttccggtag taccgacggt cgcgatagcc tgtccctgag aaaccttgtt tcccttacgg
     1321 accgcgaggg agctgcagtg agcgtacagg gtcgaatgac cgtctccgtg gtcgatgacc
     1381 acaactctgc cgtagccacc catccagccg gagtactcca cacgtcctcc gcgcgccgcc
     1441 tgtattgttc tgccccgggg ggccttgata tcaatccccg tgtggaagtc gcgccggcgt
     1501 gtcaccggat gacgcctcca cccgaacgga ctgttgatct ttcccaccac aggccaccga
     1561 taactccgcg aggagctgct ggaaaccttc gccggggcag acttcgagga tcgagctgcc
     1621 gaacgggagg aggagctcga agagggtgaa gattccaccg cttcgggcct ggctccgggg
     1681 aggaagatct ccttacccac ggggaaaggg ttcagcgcag cttcgccgtt tgcctgcctg
     1741 attctgtccg cggctatctc gtactttttc gcaacgcctg cgagcgtttc gccgctcttg
     1801 attttatgaa agatgccgtc ctgattcgga attcgcaagg tcgccccggg acgtattacg
     1861 ttggggttcc gcagatcgtt gcagccgaaa agcgtgtcaa tctctatatt ctgcgagttc
     1921 gcgatcgacc agaggctgtc gccttccgcg acaacatacg cggtgatttt gatgggaagg
     1981 accttttccc gggcggccac cagacgcgct tttcttgtga gcacctcctc caacgtcgct
     2041 tctaccgctg cgctgttatt cggaatcagg agaagctgtt tctcggagag acggtttgca
     2101 tttttcagtt cgttcgcctt gacgatatcg ctcaccgcaa cgccgtgggc aaccgctata
     2161 tcggaaagcg tctgccccgc cttcacgata tgttccttcc agagtacgga gacctgagca
     2221 agcgtcacat cttcagcttc ttccgcgtct tctcccgctt cgagcggcac ttttttaccg
     2281 tcgtccccta cgctgagcac aaccgcctcg aagtccgaag cgtcggaaac atccgaagac
     2341 gaactctccc ctcggttttc ggaggatgct ttgaaagaaa caagacgaga gacggcggaa
     2401 tcggcaaaaa gagcgccgtt ggcagttgag aacgagctct cgccgggaac aaatccatgg
     2461 gaaagccgcg gatggaactc cgtcatccgg gaaacgccga atgttgtgaa cagactgttg
     2521 ccgccaagct gcccttctcg ctctaccagg cggtcgggga agcggaaagc gccctccgag
     2581 gaggccgatt cctcttctcc caacgtcatt gatgaagacg caaaccctct gctgaactgc
     2641 tccgaaatcg accagctgaa atccgtcatc ggcagagaag cttccgaacc tgaggatact
     2701 tctttcggct gcgacgtatc gacgtcgagc ttctgcacgg aattctccgc gacatccttt
     2761 gtttttttcg cacttgcggg gacttcgttt cccagaggaa gcagtccggg ttctcctctt
     2821 ctgccccgga gagcaaagag cgaatcggtt tccattccaa gctcgtagcc gtcctcatca
     2881 agcaggacgt tcgacatttc gacgacgata aagcccgagg aagggagaat gaattctcct
     2941 gagtcctttg aatccgatag attcccccac gccgcgccaa cagttcgatc cgcggtgctc
     3001 aacgtcagaa aggcggcggt agccagcatg acaataatta caagaaaaca actaatccgg
     3061 ccaagccgca ccccttttgg cgattccgcg aaaggcagaa accccgcccc tttttttctc
     3121 ggcgcctttt tgttccgtac ctcaaacggc gaaggagagt tccttttctt caatctcctc
     3181 aacctcctct gctttgtata aagtatcgtc cttccacccg tcgccgcaga ccctcggcgg
     3241 gaagggcaca gacagcccct accctttttt gatggtcagt aaaaattcct tatttgtgga
     3301 ggactgtttc agtttatcga tgatcagatt cagcgcgccc gactcgtcga cgttcacaat
     3361 ccgccggcgg agcagccaga gacggctcag ttcgtcttcg gtgaagagca gctcctcgcg
     3421 gcgcgttccc gacttcacaa tgtcgatcgc agggaaaatc cgctgttcgg agagcttcct
     3481 cgaaaggtga agctccatat ttcccgtgcc tttaaactcc tcgtagatca cctcgtccat
     3541 tcggctgccg gtatccacca aagcagtgcc gatgatggtg aggcttccgc cttcttcaat
     3601 attcctggct gctccgaaga agcgcttcgg aaagtagagc gccgcaggat ccataccgcc
     3661 tgagagcgtt cgtccggaag gaggaacaac aaggttggat gcgcgggcga ggcgtgtgat
     3721 cgaatccaga aggagaacga catccttgcc cacctcgaca aggcgcttgg ccttctcgag
     3781 agcgagattc gccactctga gatgctcgtc cgccggacgg tcgaaggtcg aggcgatgat
     3841 ctccccgtcg actgaacgtg ccatatccgt cacttcttcc ggacgttcgt cgataagcag
     3901 caccatcaga atgatatccg gatagttcac cgtgactgcg ttggcaattt tcttgagcag
     3961 cgtggtcttc cccgctttgg gaggagaaac cagcagcgcc ctctgcccct ttccaatagg
     4021 agcgaagagg tcgacaagcc gcgtggatat ctctttcgga tccgtctcaa gggaaagttt
     4081 ttcatccggg aaaatcggcg tgatggcgcc gaaatgcggt ctttttcttg ccgcctccgg
     4141 gtcggagaag ttgaccatct ccactctgag caacgcctcg tagtgctccg aatcacgggg
     4201 gggacgcacg agcccccaga tgacgtctcc gttccgcaag ccaaaacgcc gaacctgcga
     4261 cgccgaaacg tacacgtcgt gatcgctggg cagcaggcct ttcggacgaa ggaaaccgta
     4321 cccttcctgc agaatttcaa gggttcctcc gccaaaacgg tagttgagct cttcagcttg
     4381 ggccttcagg atggcgatga caagatcatc cttgcgtagg gaggaaaaac cgactatacc
     4441 cagctctttc gctattttgc gaagatcggg attgagaaga gcggccagat gcccgtatgt
     4501 atgccttggt ttcggaggag tcttcgacgc atcatccgtc tccgaaggag tcaccgtctc
     4561 ctgaacggcg acttcggaag cattgtccgc tgtg
//
LOCUS       DAOTIL010000098         4588 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminobacteriaceae bacterium isolate AMR_MDS_3026
            NODE_22358_length_4588_cov_3.275535, whole genome shotgun sequence.
ACCESSION   DAOTIL010000098 DAOTIL010000000
VERSION     DAOTIL010000098.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868050
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminobacteriaceae bacterium (wastewater metagenome)
  ORGANISM  Aminobacteriaceae bacterium
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae.
REFERENCE   1  (bases 1 to 4588)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4588)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:19:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,224
            CDSs (total)                      :: 3,198
            Genes (coding)                    :: 3,184
            CDSs (with protein)               :: 3,184
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4588
                     /organism="Aminobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22358_length_4588_cov_3.275535"
                     /isolate="AMR_MDS_3026"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:3034609"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1888
                     /locus_tag="P5201_03155"
     CDS             <1..1888
                     /locus_tag="P5201_03155"
                     /inference="COORDINATES: protein motif:HMM:NF013245.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HRV97562.1"
                     /translation="RKRLNIALELIREPAVLLVDEPTSGLSSADSLHVVSLLKAQAAA
                     GKLVLAVIHQPSSDVYKMFDRLWMLDTGGRPIYDGNPLDALVHFRSASYRAGEQEYAC
                     PRCGNVNPEQLFEIVEEKTVDERGFPTKQRKVSPEQWRLLHLKTTLPAALPAPPTPEN
                     DETNLTPPPLREQFAVFFLRTMKSRLANRIYRVSALLQPLLLALAAGLLFRGSWGGEY
                     VFRNNNNLPGYFFISTVIAVFLGMALSADEINRDRKILERERLLRLSRGAYLASKFAW
                     LLLAAALQTGIYTLLGNGLLHIPSSGFATWAILFSTAYCAAMIGLILSDTLSSSSAIH
                     ILIPVLLAPQIMLGGPTIPFDELLRKDAGNRNVPLIAEIMPTRWGYEALMVEHYRNNP
                     FQKTFFHDETREKWFDFLTGVYIPEVRGLASYPFSPTLDIAGIPADADKAGRRLKILR
                     NELDYLERFTGLQWRGEGLNDGDLVLERYTREVQRKVMARLKEAEAVLKPLREETAQR
                     RVATEDALRGMLGHKGFEEFKNRSFNREVEKQLLGISAEDAIVISGPRLVPKVLPVVM
                     EPESRFGRAHLFAPFKRLGDFKVQTPFFNIGVLWLLSALLAAALRTRLVPRAATLFRR
                     FTGR"
     gene            complement(1939..>4588)
                     /locus_tag="P5201_03160"
     CDS             complement(1939..>4588)
                     /locus_tag="P5201_03160"
                     /inference="COORDINATES: protein motif:HMM:TIGR03797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015440 - ABC-type peptide transporter
                     activity [Evidence IEA];
                     GO_process: GO:0043213 - bacteriocin transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="NHLP bacteriocin export ABC transporter
                     permease/ATPase subunit"
                     /protein_id="HRV97563.1"
                     /translation="LRISRSLLLEQTDGEGTAIAAFLADRFLEGLGRGIVKDIVPLPR
                     IKQSIPAGRTELGPNRPAGSETLLWTEMEEGRALFVGTEDLAPGSGLFALPPNCWIVP
                     LEQSVVRTTPTEELLRRGEFASVFDAFCEMIFSSLAMNARLRAADDLGALRQMQKSSR
                     KALSSALGTLGEALSGKRRKVAASREDSPLRRAFSTVAAFLGRSVAAPKGEELASERG
                     ESLRDLARASNLQLRRVTLAGPWWISDGAPLIAYVQENAEKSSGGGEMSGTPVALLPS
                     GRGYRLVDDEGERRVDERIAATLGQDAIQVYRPLPGSPLGGMDLLRFGLAGTKRDMGA
                     VLVISVCLALLGMISPEVNRQIFGEVIPQAERGRMLQLFGILVSVALSSGLLHLANGT
                     AFLRTESVMDHDVSAGIWDRLLRLPVGFFRKTTSGDLANRALGLFMLRETASRTVKTL
                     LVQSVFLIFFLGQSFWYDWRLALSGLACLFVPAALTVVVNVVQLRFQRKMIGLQNRLL
                     ALTFQIMTGISKIRVAGAEERSFARWAELFGAQRTLGTSARRLEVFLNTVMALFPLSV
                     AIVILFALVRWSAADASYETGRFMAFWSAFGALQGAFFQMVSSVTTTLNMLPMVENLA
                     PILQAEPETGDLKTDPGEITGRIDLEGVVFRYLKEGPPVLKGLDLHIEPGEFVAVVGP
                     SGAGKSTLIRLLLGFDQPESGGIFYDNKDIAELDLGKLRRQIGVVLQSGGLLSGDIAS
                     NVRCSRNMTVEQIEDALRTAGMEEDIAAMPMGIHTVITDGANTLSGGQKQRLLIARAV
                     AGKPRVLIFDEATSALDNRTQAMISRSLESIQATRIVVAHRLSTVIGADRICVLEDGR
                     LVEEGKYDELMAKDGLFAELVRRQTV"
BASE COUNT          880 a         1522 c         1393 g          793 t
ORIGIN      
        1 gagaaaacgc ctcaacatcg ctctggaact catcagggaa cctgcggtgc tcctcgtgga
       61 cgagcccacc agcgggctct cctccgcgga ctcgctgcat gtcgtctcgc tgctcaaggc
      121 tcaggccgcc gccggaaaac ttgtcctcgc cgtgatccac caaccctcgt cggacgtcta
      181 caagatgttc gacagactct ggatgctcga caccggaggg cgtcccatct acgacggcaa
      241 ccccctggac gcgctcgtcc atttccgaag cgcctcctac cgcgcgggcg aacaggagta
      301 cgcctgcccc cgctgcggga acgtcaaccc ggagcagctt ttcgagatcg tcgaagagaa
      361 gaccgtcgac gagcgggggt ttccgacgaa acagcggaag gtctcccccg agcagtggcg
      421 cctccttcat ctgaaaacaa cgctcccggc cgcattgccc gcgccgccga cgccggagaa
      481 cgacgaaacg aatctcaccc ctccgccgct gcgcgagcag ttcgccgtct tctttctgcg
      541 gacgatgaag agtcgtctcg cgaacaggat ctaccgcgtt tcggcgctcc tccagccgct
      601 gctgctcgct ttggccgcag gcctcctctt cagaggttca tgggggggcg agtatgtctt
      661 caggaacaac aacaaccttc cgggatattt cttcatcagc acggtgatcg ccgtctttct
      721 cgggatggcc ctcagcgcgg acgagatcaa cagggacagg aagatcctcg aacgcgagcg
      781 cctgctccga ttgagcaggg gcgcgtatct cgcctccaag ttcgcgtggc tcctcctcgc
      841 ggcggcgctc caaacaggaa tctacacgct tctgggcaac ggcttgctcc acatcccctc
      901 gtcggggttc gccacgtggg ctatcctctt cagcacggca tattgcgcgg ccatgatcgg
      961 gctgatcctg tcggacacgc tgtcctcgtc ctcggccatt cacattctca tccccgtgct
     1021 gctggcgccg cagatcatgc tcggagggcc gacgatcccc ttcgacgagc tgctccgcaa
     1081 ggacgccgga aaccgaaacg ttcccctgat cgcggagatc atgccgacgc ggtgggggta
     1141 cgaggcgctc atggtggagc actacaggaa caacccgttc caaaagacgt tttttcacga
     1201 cgagacgcgg gagaagtggt tcgatttcct cacaggggtg tacatccccg aggtacgcgg
     1261 cctggcgagc tacccgttct ctccgacgct cgacatagcc gggatccccg cggacgccga
     1321 caaagcgggc cgacggctga aaattctgcg gaacgagctc gattatctgg agcgctttac
     1381 gggcctgcaa tggcggggcg agggactgaa cgacggcgat ctcgttttgg agaggtacac
     1441 tcgcgaggtc caacggaagg tgatggcgcg gctcaaagag gcggaagcag ttctcaaacc
     1501 cctccgcgaa gagaccgcgc aacgccgggt cgcgaccgaa gacgcgcttc gcggaatgct
     1561 cggacacaag gggttcgaag agttcaagaa caggagtttc aacagagagg tcgaaaaaca
     1621 gctcctcggc atctccgccg aggacgcgat cgtcatatcc gggccgcgcc tcgtcccgaa
     1681 agtcctcccc gtcgtcatgg agcccgagtc gcgcttcggc agggcgcacc ttttcgctcc
     1741 gttcaaacgg ctcggagatt tcaaggtaca aacgccgttt ttcaatatcg gcgtcctctg
     1801 gctcctctcc gcacttctgg ccgcggctct gcggacgcgc ctcgttccgc gcgcggcaac
     1861 gctcttccgg agattcacag ggagataacg ccacgtcccc aaaaacgagc cgcaggaatg
     1921 ccttcagtcc gttttcttct acaccgtctg tcttcggacc agttcggcga agagaccgtc
     1981 cttcgccatc agttcgtcgt acttcccctc ctcgacaaga cgcccgtcct cgaggacgca
     2041 gatacggtcg gcgccgatga ccgtggagag ccggtgcgcc acgacgatgc gcgtcgcctg
     2101 gatgctctcc aagctgcggc tgatcatcgc ctgcgtccgg ttgtccagcg cgctcgtggc
     2161 ctcgtcgaag atgaggacgc gcggcttccc ggcgacggcg cgggcgatca gaaggcgctg
     2221 cttctgccct ccggagagtg tgttcgcgcc gtccgtgatg accgtatgaa tccccatcgg
     2281 catcgcggcg atatcctcct ccatccccgc cgtccgcagc gcatcttcta tctgctcgac
     2341 cgtcatattg cgggagcagc ggacgttgga ggcgatatcc ccggagagca gaccgccgct
     2401 ctggaggacg acgccgatct gccgccgcag cttccccagg tccagctcgg cgatgtcctt
     2461 gttgtcgtag aagatgcccc ccgactccgg ttgatcgaac ccgagcagca gacggatgag
     2521 ggtggacttg ccggccccgg atgggcccac cacggcgacg aattcccccg gttcgatgtg
     2581 gaggtcgagc cccttcagca cgggaggccc ctccttcagg tagcggaaga ccaccccttc
     2641 caggtcaatc cgtcccgtga tctctccggg atcggtcttc agatcgccgg tctcgggctc
     2701 ggcctggagg atgggggcga ggttctccac catcgggagc atgttcaacg tagtggtgac
     2761 cgaacttacc atctggaaga aagcgccctg cagcgcgccg aaggcggacc agaaggccat
     2821 gaaacgcccc gtttcgtacg aagcgtccgc ggcgctccag cggacaaggg caaagagaat
     2881 gacgatggcg acggagagag gaaagagcgc cataaccgtg ttcaaaaaga cctccaggcg
     2941 tctcgccgaa gtccccagcg tccgctgcgc cccgaacagc tccgcccatc gtgcgaagga
     3001 gcgctcttcg gcgcccgcca cccggatctt cgatatgccg gtcatgatct ggaaggtcag
     3061 cgcaagaagc ctgttctgca gcccgatcat cttgcgctgg aagcgaagct gcacgacgtt
     3121 caccaccacg gtcagcgcgg ccggaacgaa caggcacgcc aggccggaga gggcgagcct
     3181 ccagtcgtac cagaacgact gccccagaaa gaagatcagg aaaacgctct ggacgagcag
     3241 cgtcttgacg gttctcgacg cggtctcgcg gagcatgaag agaccaagcg cccggttcgc
     3301 gagatcgccg gacgtcgtct tccggaagaa gcccaccggc aggcgaagca agcggtccca
     3361 gattcccgcc gaaacgtcgt ggtccatcac gctctccgtg cggagaaagg ccgttccgtt
     3421 cgccaggtgg agcaggcccg acgaaagcgc gacggagacg agaataccga agagctggag
     3481 catccttccc cgttcggcct gcggaatgac ttcgccgaag atctggcggt tcacctcagg
     3541 ggagatcatc cccagaaggg ccaggcagac cgaaatgacg aggacggcgc ccatgtctct
     3601 cttcgtcccg gcaaggccga aacggagcag atccatgccc cccagcggcg agccggggag
     3661 cggacggtag acctggattg catcctgccc gagagtcgcg gcgattcgct cgtcgacacg
     3721 acgctctccc tcgtcgtcga caaggcggta tccgcgtccg gacggaagga gcgcgacggg
     3781 cgtcccgctc atctctcccc ctccggaaga cttttccgca ttctcctgta cgtaggcgat
     3841 cagcggcgca ccgtccgaga tccaccaggg accggcgagc gtcacgcgac gaagctggag
     3901 gttggaagcg cgcgcgaggt cgcgaagcga ctccccgcgt tcggaggcga gttcctctcc
     3961 tttcggagcg gcgacgctcc gccccaaaaa cgcggcgacc gtggaaaagg ccctccggag
     4021 cggcgaatcc tcacgcgagg cggccacttt tctgcgtttt cccgaaagcg cctcgcccag
     4081 cgttccaaga gcggaggaaa gagctttcct gcttgatttc tgcatctgcc ggagcgcccc
     4141 aaggtcgtcg gcggcccgga gccgggcgtt catcgccagc gaggagaaga tcatctcgca
     4201 gaatgcgtcg aaaacggagg cgaactcgcc cctgcggagc agctcttccg tcggggtcgt
     4261 ccgcactacg gactgctcca gcggaacgat ccagcagttc ggaggaaggg cgaagagtcc
     4321 gcttcccggc gcaaggtcct cggtgccgac gaaaagcgcg cggccttcct ccatctctgt
     4381 ccagagcagc gtttcacttc cggccggcct gttcggcccg agttccgtcc ttccggcggg
     4441 aatggactgt tttatgcgcg gaagcggtac gatatccttg acgatgcccc gccccagccc
     4501 ctccaaaaac ctgtcggcaa ggaacgccgc aatcgcggtt ccctctccgt ccgtttgttc
     4561 gagaagaagc gatctggaga tccgcagc
//
LOCUS       DAOTIL010000099         4582 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminobacteriaceae bacterium isolate AMR_MDS_3026
            NODE_22393_length_4582_cov_3.804506, whole genome shotgun sequence.
ACCESSION   DAOTIL010000099 DAOTIL010000000
VERSION     DAOTIL010000099.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868050
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminobacteriaceae bacterium (wastewater metagenome)
  ORGANISM  Aminobacteriaceae bacterium
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae.
REFERENCE   1  (bases 1 to 4582)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4582)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:19:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,224
            CDSs (total)                      :: 3,198
            Genes (coding)                    :: 3,184
            CDSs (with protein)               :: 3,184
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4582
                     /organism="Aminobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22393_length_4582_cov_3.804506"
                     /isolate="AMR_MDS_3026"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:3034609"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1408)
                     /gene="glpK"
                     /locus_tag="P5201_03165"
     CDS             complement(<1..1408)
                     /gene="glpK"
                     /locus_tag="P5201_03165"
                     /EC_number="2.7.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006582891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004370 - glycerol kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006071 - glycerol metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol kinase GlpK"
                     /protein_id="HRV97564.1"
                     /translation="MSEKKYVVAIDQGTTSSRAIVFDLEGTPKNSHQMEHEQIYPKPG
                     WVEHNPMEIWSRVQDVIREVLNTNGVKADEIGAIGITNQRETTVVWEKATGKPVYNAI
                     VWQCMRTQDFCAEWQKKPGWDKKVNDLTGLVISPYFSGTKIKWILDNVPGVRAKAEKG
                     EVLFGNIDTWVTWNLTGGPNGGVHVTDVTNASRTLLMNIETLKWDKEMMDFLGVPEAM
                     LPAIKPSSYVYGYTPESGPFGAKIPVSGILGDQQAALFGQTCYSPGEAKNTYGTGCFM
                     LLNIGDKPVRSKNGLLTTVAYGLEEGKATYALEGSIAITGAAVQWLRDNLRLFDDAPD
                     SEYFAAKVKDSGGIYFVPAFSGLYAPYWDMSARGAILGLTRYITKEHIIRATLESICY
                     QTRDVQAAMDADSGVPLKALKVDGGAVKNNLLMQMQADILGVPVVRPVVNETTALGAA
                     YAAGLATGLWKSTEELRKH"
     gene            complement(1571..1969)
                     /gene="dhaM"
                     /locus_tag="P5201_03170"
     CDS             complement(1571..1969)
                     /gene="dhaM"
                     /locus_tag="P5201_03170"
                     /EC_number="2.7.1.121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009201941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047324 - phosphoenolpyruvate-glycerone
                     phosphotransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxyacetone kinase phosphoryl donor subunit
                     DhaM"
                     /protein_id="HRV97565.1"
                     /translation="MNAILVVSHSAEAARGIVAIASQMAGERVGIAACGGTDDGELGT
                     SVPSILSALEGLLETSDGVLVIPDLGSAVLAARTAKDLLGERAGRVLIADGPVLEGTL
                     MASVEASVGAGLDRLAAVVDETRGLRKLQE"
     gene            complement(1970..2608)
                     /gene="dhaL"
                     /locus_tag="P5201_03175"
     CDS             complement(1970..2608)
                     /gene="dhaL"
                     /locus_tag="P5201_03175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014807547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004371 - glycerone kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006071 - glycerol metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxyacetone kinase subunit DhaL"
                     /protein_id="HRV97566.1"
                     /translation="MNLDVQGMLKVFDAIGETMAAHKEYLTELDSAIGDADHGINMNR
                     GFHKVHDKLSAGSYTDLGALFKDVGMTLMGNVGGASGPLYGTFFMRMSTKLAGRQDVD
                     TVSLADAMEEGLKGIVALGKAQLQDKTMVDALTPAVEALKKASDNGVDLCGALEAGVA
                     AAQSGMESTIPLVARKGRASYLGERSAGHQDPGATSIYLILSTILRSVKESS"
     gene            complement(2619..3617)
                     /gene="dhaK"
                     /locus_tag="P5201_03180"
     CDS             complement(2619..3617)
                     /gene="dhaK"
                     /locus_tag="P5201_03180"
                     /EC_number="2.7.1.121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006301605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004371 - glycerone kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006071 - glycerol metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxyacetone kinase subunit DhaK"
                     /protein_id="HRV97567.1"
                     /translation="MKKLLNKVDDVVVESLAGLAAAHPGLVKLHPSVKAVLRADAPQK
                     KVAILSGGGSGHEPLHGGYVGYGMLDAACPGEVFTSPTPDQMYEAAKAVDGGKGVLFI
                     VKNYTGDVMNFQMAADLLAADGIPVAQVVIDDDVAVQDSLYTAGRRGVGGTVLIEKIV
                     GAKAEAGASLEEVKALCEKVNSQVRSMGMALTSCAVPAAGKPTFELGEDEMEIGIGIH
                     GEPGRERMKLKTAKEIVEMMAKAIVDDLPFKAGDEVVAFVNGMGGTPQMELYLAYNDL
                     VNFLKGKGIVIARSLVGNYITSLEMQGFSITLLKLDGEMKALWDAPVETAGLRWGR"
     gene            complement(3723..4460)
                     /locus_tag="P5201_03185"
     CDS             complement(3723..4460)
                     /locus_tag="P5201_03185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006301606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MIP/aquaporin family protein"
                     /protein_id="HRV97568.1"
                     /translation="MTNLLGEFFGTMTLVVFGDGLVACALLNKSKGQNAGWVHITIGW
                     GAAVVMGVFAATSLGAPQADINPAVTLAKMLAGTYTLPHAMMTMLAQVGGGILAGVVV
                     WLAYLGHWEATEDKGLKLAVFSTGPAIRNPINNFITEMIGTIFLVVPIMFIFSSGVKE
                     ALGGLTPGFGPFLVGWLVMVIGMALSGPTGYAINPARDLGPRIAHFVLPIAGKGDSDW
                     GYAWIPVLAPLTGGAVSYFIAHALGVM"
BASE COUNT          861 a         1380 c         1397 g          944 t
ORIGIN      
        1 agtgcttgcg cagctcctcg gtgctcttcc agagaccggt ggcaagtcct gcggcgtatg
       61 ctgcgccgag cgccgtggtc tcgttgacga ccgggcggac gacgggcacg ccgaggatgt
      121 cggcctgcat ctgcatgagg aggttgttct tgactgcgcc gccgtcgacc ttcagggcct
      181 tcagaggtac gccggagtcg gcgtccatag ccgcctggac gtcgcgggtc tggtagcaga
      241 tgctctccag cgtcgcgcgg ataatgtgct ccttggtgat gtagcgggta aggccgagga
      301 tggcgccgcg ggcgctcata tcccagtaag gggcgtagag cccggagaac gcggggacga
      361 agtagatgcc gccgctgtcc ttcaccttgg ctgcgaagta ctcggaatcg ggggcgtcgt
      421 cgaagaggcg cagattgtcg cgcagccact ggactgcggc gccggtgatg gcgatggatc
      481 cctcaagagc gtaggtcgcc ttgccctctt caaggccgta ggcaaccgtc gtcagcagac
      541 cgttcttgga gcggaccggc ttgtcgccga tgttcagcag catgaagcag ccggtgccgt
      601 aggtgttctt cgcttcgccg gggctgtagc atgtctggcc gaagagcgcc gcctgctggt
      661 cgccgaggat gccggatacg gggatcttcg cgccgaaggg gccgctctcg ggggtatagc
      721 catagacgta gctgctgggt ttgattgcgg ggagcatcgc ctcgggaacg ccgaggaagt
      781 ccatcatttc cttgtcccac ttcagcgtct cgatgttcat gaggagggta cgggaggcgt
      841 tggtgacgtc ggtgacgtgg acgccgccgt tggggccgcc ggtgaggttc caggtaaccc
      901 atgtgtcgat gttgccgaag agaacctcgc ccttttccgc cttcgcgcgg acgccgggga
      961 cgttgtcgag aatccacttg atcttcgttc cggagaaata ggggctgatg acgaggccgg
     1021 tcagatcgtt gaccttcttg tcccagccgg gcttcttctg ccattcggcg cagaagtcct
     1081 gcgtcctcat gcactgccag acgattgcgt tgtagaccgg cttgccggtg gccttctccc
     1141 agacgacagt ggtttcgcgc tggttcgtga tgccgatggc gccgatctcg tccgccttga
     1201 ctccgttcgt gttcagtacc tcgcggatga cgtcctgcac gcgggaccag atctccatcg
     1261 gattgtgctc cacccagccc ggcttcggat agatctgctc gtgctccatc tggtgcgaat
     1321 tcttcggagt tccctcaagg tcgaaaacaa tggctcggct gctggtcgtt ccctggtcta
     1381 tggccacaac gtatttcttc tcggacatga cagtacctcc tgttttgggt ttatcgaggc
     1441 gtgagaacaa gttgctgaac cagttgggca tgatcgtacc gcctccttta tttgcgatgc
     1501 cgccgagacg tgaacgccac tatagcgcct cccggctgtt tcgcgtcata cacgagctat
     1561 gaacagagcc ttattcctgt agctttctca atcccctggt ttcgtccacg acggcggcga
     1621 gccggtcaag ccccgccccg acgctggcct ccaccgaggc catgagggtt ccctccagaa
     1681 cagggccgtc ggcgatgagt acgcggcctg cacgttctcc aaggagatcc ttggccgtgc
     1741 gcgccgcaag aacagcgctc cccaggtcgg ggatgacgag aacgccatcg gatgtttcga
     1801 gcagcccttc aagcgcagag agaatcgacg gaaccgaggt tccgagttcg ccgtcgtcgg
     1861 ttccgccgca tgcggcgata ccgacccgct cccctgccat ctgcgaggcg attgccacaa
     1921 tgccccgcgc agcttcggcg ctgtgggaaa cgacgagaat ggcgttcact caggaactct
     1981 cctttacgga gcgcagaatc gtcgagagaa tcaggtagat ggacgtcgct ccgggatcct
     2041 ggtgtcccgc cgaacgctcg ccgaggtagc ttgcccgccc tttgcgggcg acaaggggaa
     2101 ttgtcgactc catacccgac tgcgccgcag cgacgcccgc ttcgagagcg ccgcaaagat
     2161 caacgccgtt gtcggaagct tttttcaaag cctcgactgc cggcgtcagc gcgtcgacca
     2221 tggtcttatc ctgaagctgg gctttgccaa gggcaacgat gcccttaagc ccctcttcca
     2281 ttgcatcggc cagggagacg gtatccacat cctgccttcc ggcgagtttc gtcgacatgc
     2341 gcatgaaaaa cgttccgtag agaggtcccg aagcgccgcc gacatttccc atcagggtca
     2401 tgccgacatc cttgaaaaga gcgccgagat ctgtgtagga tcctgcggag agcttgtcgt
     2461 gaaccttgtg aaaaccgcgg ttcatgttga ttccatggtc ggcgtcgcct atagccgaat
     2521 cgagttcggt caggtattcc ttgtgcgccg ccatcgtctc gccgatagcg tcgaacacct
     2581 tgagcattcc ctggacatcg aggttcatct ctgcgcgcct acctccccca gcgaagcccc
     2641 gccgtctcga cgggagcgtc ccaaagggcc ttcatttctc cgtcgagttt cagcaaggta
     2701 atggagaaac cctgcatttc gaggcttgtg atatagttgc cgacgagaga acgtgcaatg
     2761 acgatcccct tccccttgag gaaattgaca agatcgttgt atgcaaggta gagttccatc
     2821 tgcggcgtgc cgcccatgcc gttgacaaag gcgacaactt cgtcgcccgc tttgaatgga
     2881 agatcgtcga cgatcgcctt ggccatcatc tccacgatct ccttcgccgt tttcagcttc
     2941 atgcgctcac ggccgggctc gccatggatt ccgatgccta tttccatctc gtcctcgccg
     3001 agctcgaagg tcggtttgcc cgcagccgga acggcgcacg atgtgagcgc catgcccatg
     3061 gagcgaacct gactgttgac cttttcgcag agcgccttaa cctcttcgag cgaagcgccc
     3121 gcttccgcct tggcgccgac gatcttttcg atcagaacag tgccgccgac tccgcggcgt
     3181 cccgccgtat acagcgaatc ctgaacggcg acgtcgtcgt cgatgacgac ctgcgcgacg
     3241 ggaatgccgt ccgcagcaag aagatcggcg gccatctgga agttcatgac gtcgcccgtg
     3301 taattcttca cgataaagag gacgccctta ccgccgtcca cggccttggc cgcctcgtac
     3361 atctgatcgg gagtgggaga ggtgaacact tcgccggggc aggccgcgtc gagcataccg
     3421 taccccacgt accctccgtg aagaggttcg tgcccgctgc cgccgccgga gaggatggca
     3481 accttttttt ggggcgcgtc ggccctcagg acagccttga ccgacgggtg gagcttcacc
     3541 agaccgggat gcgccgcggc gagaccggcg agcgattcga cgacgacgtc atccactttg
     3601 ttgaggagct ttttcactaa acaacgcctc ctttcaagga gcaggttcta ttccggagaa
     3661 ccggcggagg aggcgtttgt gtttctcccc cgccggccta atttcgaaaa gaaaaccgtg
     3721 cgttacatca cgccgagagc gtgtgcgatg aagtaggaga ctgcgccgcc ggtaagaggc
     3781 gcaagcacgg ggatccaggc atatccccag tccgaatcgc cttttccggc gatcgggagt
     3841 acaaagtgcg caatcctggg gccgaggtcg cgggccgggt tgatggcgta tccggtgggg
     3901 ccgctgagtg ccataccgat aaccatgacg agccagccga cgaggaaggg accgaaaccg
     3961 ggggtaaggc cgccgagggc ttccttgacg ccggaggaga agatgaacat gattggaaca
     4021 acgaggaaaa tggtaccgat catctcagtg atgaagttgt tgatggggtt gcggatcgcg
     4081 ggtccggtgg agaagacggc cagcttgagc cccttgtcct cggtggcttc ccagtggccg
     4141 aggtaggcaa gccatacgac gacgcctgcg aggataccgc cgccgacctg cgccagcatg
     4201 gtcatcatcg catgcggcag ggtgtaggtt ccggcgagca tcttggccag tgtcactgcg
     4261 gggttgatgt cggcctgagg cgctccgagg gatgtcgcgg cgaagacgcc cattaccact
     4321 gccgcgcccc atccgatggt gatgtgcacc catccagcat tctgtccctt ggacttgttg
     4381 agcagcgcgc aggcgacgag gccgtcgcca aaaacaacca gagtcatggt tccgaagaat
     4441 tcacccagaa gattcgtcat cgcatttcat acctccttcg taaaataaaa acaaagaacg
     4501 gtagctcaga aacacactat tacatcctta ctcaatgctt ggcatccctc ctcacatacg
     4561 gttccgcagg aacctcaggg ca
//
LOCUS       DAOTIL010000100         4581 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminobacteriaceae bacterium isolate AMR_MDS_3026
            NODE_22401_length_4581_cov_3.659744, whole genome shotgun sequence.
ACCESSION   DAOTIL010000100 DAOTIL010000000
VERSION     DAOTIL010000100.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868050
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminobacteriaceae bacterium (wastewater metagenome)
  ORGANISM  Aminobacteriaceae bacterium
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae.
REFERENCE   1  (bases 1 to 4581)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4581)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:19:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,224
            CDSs (total)                      :: 3,198
            Genes (coding)                    :: 3,184
            CDSs (with protein)               :: 3,184
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4581
                     /organism="Aminobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22401_length_4581_cov_3.659744"
                     /isolate="AMR_MDS_3026"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:3034609"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..207)
                     /locus_tag="P5201_03190"
     CDS             complement(<1..207)
                     /locus_tag="P5201_03190"
                     /inference="COORDINATES: protein motif:HMM:NF016858.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRV97569.1"
                     /translation="MSRREILGVRIKLASPERIRELSSGEVKKPETINYRTLRPEKDG
                     LFCERIFGPTKSFECACGKYKYCGP"
     gene            complement(207..3824)
                     /gene="rpoB"
                     /locus_tag="P5201_03195"
     CDS             complement(207..3824)
                     /gene="rpoB"
                     /locus_tag="P5201_03195"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009201495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000345 - cytosolic DNA-directed RNA
                     polymerase complex [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta"
                     /protein_id="HRV97570.1"
                     /translation="MAEFVPVSSKRQRLTFGRARDLVEIPDMIEVQRDSYNWFYQESR
                     EADMRTRQGLQELLQEVFPIESYDGSFALEFVRYILDDPSTEEEEARQRDLTWSRPVR
                     ATIRLVNRKTRELKEEEIFLGDFPIMTGRGTFVINGTERVVVNQLARSAGIYFTKDEM
                     IPGQEAYSAKIIPERGAWLEFALSHGEPITVNIDNRKKIPATLLLKIFGAGSNEGVLE
                     LFNARTVEKDVSEDDVRGLLSAESVLDPDGGVILPRNRLITKDHLEALWDMGRTKVRV
                     WDADPALALTLERDNAANADDAMVELFRKLRPNEPARIENAREYVHTLFFDPRRYNLG
                     RVGRYKMNRSLGIAISEEIRLLQLEDLAKIVTGLLTLKEPEKIGDDIDHLGNRRVRSV
                     GELLANQIRIGLLRMERIAKERMTTIPDLGAAMARDLINVRPIAASLREFFGSGQLSQ
                     FMDQTNPLAEVTHRRRLSALGPGGLSRERAGFEARDVHYTHYGRVCPIETPEGPNIGL
                     VTSLATYARLNEYGFLITPRRKVLNGRVTGEIVFLSADEEDGVYVGMANTPVDDEGVI
                     TTAECFTRYQGNIVTVPREEVSYLDVSPKQIVSSSTALIPFLEHDDANRALMGSNMQR
                     QAVPLIAPQAPVVGTGMEHRIAKDSGSCVVARKNGEVEYVDSTRIEIRSGVETIVYPM
                     IKFRRSNQGTIIHQKPLVRKGDSVSAGEIIADGQAVEGGELALGRNVLVAFMAWEGYN
                     FEDAILLSERLVKEDFYSSIHIEEYEIEARDTKLGPEEITRDIPNVGEDVLKNLDDEG
                     IVRVGAEVNAGDILVGKVTPKGESDQTPEEKLLRAIFGEKAREVRDTSLKVPHGARGK
                     VVAVKQMTRADSPDALSPGVNMVVKIYVAQLRKITVGDKMAGRHGNKGVVSRILPVED
                     MPYLPDGTPVDVVLNPLGVPSRMNLGQVLETIMGFVAMQNGWNVATPVFEGANEQEIF
                     DLLNDVAKEKYPELTSDGMITLYDGRTGDPVEKKVTVGCMYMLKLIHLVDDKIHARSI
                     GPYSLITQQPLGGKAQFGGQRFGEMEVWALEGYGAANILQEMLTVKSDDIRGRLKTYE
                     RIVKGQNLTKPGVPESFRVLVKELQGLGLDVEVKYSDGVIGELVMDEDEDDVVGSRRA
                     PRERSILQEEPEAEVKKEVVEKEKFFEDEDEVGGIVPEEMLFGGALPEGMSIEEEGDD
                     N"
     gene            complement(3955..4042)
                     /locus_tag="P5201_03200"
     tRNA            complement(3955..4042)
                     /locus_tag="P5201_03200"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(4006..4008),aa:Leu,seq:cag)
     gene            complement(4120..>4581)
                     /locus_tag="P5201_03205"
     CDS             complement(4120..>4581)
                     /locus_tag="P5201_03205"
                     /inference="COORDINATES: protein motif:HMM:NF015446.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Sua5 family C-terminal domain-containing
                     protein"
                     /protein_id="HRV97571.1"
                     /translation="TVLDATGEQLVLLRPGGCAVEEIEARSGMKVLLSGGAAHRSPGT
                     RYRHYAPRVPLLLDTDADGIARAAAETDGALRIAWIGMHAPEISLGEGVAHPGLRILF
                     DSPENYARGLFHALRVFEERGAGLIAAQWPPSEEGIGRALRDRLMRASMKE"
BASE COUNT          837 a         1455 c         1311 g          978 t
ORIGIN      
        1 gggaccacag tatttgtact ttccgcaggc gcattcaaag ctcttcgtag gaccgaagat
       61 gcgctcgcag aagagcccgt ccttttccgg ccgcagcgta cggtagttta tcgtctcggg
      121 cttcttgacc tctccgctgg agagttcgcg aatccgctcg gggctggcga gcttgatccg
      181 cacgcccagt atctctctac ggctcatcag ttgtcatccc cttcttcttc gatggacatc
      241 ccttcgggaa gcgcccctcc gaacagcatt tcttcgggaa caatcccccc gacttcatcc
      301 tcgtcttcga agaatttctc cttctccacg acttccttct tgacctcggc ctccggctcc
      361 tcctggagga tgctgcgctc tctcggcgca cggcgggatc cgacgacgtc gtcttcatcc
      421 tcatccatca caagctctcc gataacgccg tcgctgtact tcacctcgac gtcgagcccc
      481 agcccctgca gttccttcac aagcactctg aaactctcgg gaactcccgg ttttgtcagg
      541 ttctgtccct tgacgatgcg ttcgtaggtc ttgagacgcc ccctgatatc gtccgacttg
      601 acggtgagca tctcctggag gatattcgcc gcgccgtacc cttcaagcgc ccagacttcc
      661 atctctccga atctttggcc gccgaactgc gccttgcccc ccagcggctg ctgggtgatg
      721 aggctgtacg gaccgatcga gcgcgcgtga atcttgtcgt cgacaaggtg tatcagtttc
      781 agcatgtaca tgcagccgac ggtgaccttc ttttccacag ggtcgccggt gcggccgtcg
      841 tagagcgtga tcatgccgtc gctcgtgagc tcgggatact tttccttcgc aacgtcgttc
      901 agcaggtcga aaatctcctg ctcgttcgcg ccctcgaaga ccggggtggc gacattccag
      961 ccgttctgca tggcgacgaa gcccataatt gtttcgagca cctgaccgag gttcatgcgg
     1021 ctggggacgc ccagcggatt cagcaccacg tccaccggcg tgccgtccgg caggtagggc
     1081 atatcctcca cgggaaggat gcgggagacg acgcccttgt ttccgtgccg gcccgccatc
     1141 ttgtcgccga cggtgatctt gcgaagctgc gcgacataga tcttcacgac catgttcact
     1201 ccggggctca gcgcgtccgg actgtccgcg cgtgtcatct gttttaccgc gaccaccttg
     1261 ccgcgcgcgc cgtgaggcac tttcagcgat gtgtcgcgca cctcgcgcgc cttctcgccg
     1321 aagattgcgc gaaggagctt ctcctcgggc gtctggtccg actccccctt gggggtgacc
     1381 ttgccgacaa ggatgtctcc ggcgttcact tccgcgccga cgcggacgat cccctcgtcg
     1441 tcgaggttct tcagcacgtc ctcgccgacg ttcgggatgt cgcgggtaat ctcctcggga
     1501 ccgagtttcg tgtccctggc ctctatctcg tactcctcga tgtgaatcga ggagtagaag
     1561 tcctctttaa caagacgctc gctcaacagg atagcgtcct cgaagttata cccctcccag
     1621 gccatgaagg ccaccagaac gttccgtcca agcgcgagtt cgccgccttc gacggcctgg
     1681 ccgtccgcga tgatctcccc tgcggagacg gaatctccct tccggacgag aggtttttgg
     1741 tgaatgatcg ttccctggtt tgaacggcgg aacttgatca tcgggtagac gattgtttcc
     1801 accccgcttc gtatctcaat gcgggtggaa tcgacgtact cgacctctcc gttcttgcgg
     1861 gcaacgacgc acgagcccga atccttggcg atgcggtgct ccatccccgt gccgaccaca
     1921 ggcgcctgcg gcgcgataag cggcaccgcc tgacgctgca tgttcgagcc catcagggcg
     1981 cggttcgcgt cgtcgtgctc aaggaaaggg atgagcgccg tagaggacga aacgatctgc
     2041 ttcggcgaca cgtcgaggta gctcacctct tcgcgaggaa ccgtgacgat gtttccctgg
     2101 tatcgcgtga aacactcggc ggtcgtgata accccctcgt cgtcgacggg agtgttcgcc
     2161 atgccgacgt agacgccgtc ctcttcatcg gcggaaagga aaacaatctc ccccgtgacg
     2221 cggccgttca aaaccttccg gcgaggcgtg atgagaaagc cgtattcatt caggcgggca
     2281 tacgtcgcca acgaggtcac aagaccgata ttcgggcctt cgggcgtttc tatcgggcag
     2341 accctgccgt agtgcgtata gtgcacgtcg cgcgcttcga atccggcgcg ttcgcgggag
     2401 aggcctcccg gtccaagcgc ggagaggcgg cggcggtgcg tcacttccgc gagcgggttc
     2461 gtctggtcca tgaactgcga aagctgcccc gaaccgaaaa actcgcgcag gctggcggct
     2521 atggggcgca cgttgatcag atcgcgcgcc atggccgcgc cgaggtcggg aatcgtcgtc
     2581 atgcgctcct tggcgatgcg ctccatgcgc agcaggccga tgcgtatctg gttggcgagc
     2641 agttcgccga cggagcggac gcgccggttg ccgagatggt cgatgtcgtc gccgatcttc
     2701 tccggttcct tcagtgtcag gagacctgtg acgatcttcg ccaggtcttc gagctggagc
     2761 aggcggatct cctcggaaat cgcgatgccg agactccggt tcattttata gcgccccacg
     2821 cggcccaggt tataacgcct cggatcgaag aagagcgtat ggacgtactc ccgtgcgttc
     2881 tctattcgtg cgggctcgtt cgggcgcagc ttcctgaaga gttccaccat cgcgtcgtcg
     2941 gcgttggcag cgttgtcccg ctccagcgtg agcgcgaggg cggggtcggc gtcccagacg
     3001 cggaccttcg tcctccccat gtcccagagc gcttcgagat gatctttcgt gatcaggcgg
     3061 ttcctcggga ggatcacgcc gccgtccgga tcgagcacgg attcggcgga aagcagccct
     3121 ctgacgtcgt cttccgagac gtccttttcc acggtgcgcg cgttgaagag ttcgagcacg
     3181 ccctcgttcg agccggcgcc gaatatcttc aacagcagcg tcgccggaat cttcttccgg
     3241 ttgtcgatgt tcacggtgat gggttcgccg tgggagagcg cgaactcaag ccaggctccc
     3301 cgttcgggga tgattttggc ggagtacgcc tcctgccccg gaatcatctc gtccttcgtg
     3361 aagtaaattc ctgcagaacg cgcaagctgg ttcacgacga cacgctccgt cccgttgatg
     3421 acgaacgtac ctctccccgt catgatagga aagtcgccga gaaagatctc ctcttccttg
     3481 agctctctgg ttttccggtt cacaagccgg attgtcgcac ggacgggacg ggaccacgtc
     3541 agatcccgct gacgagcctc ctcctcctcc gtagaaggat cgtcgagtat gtagcggacg
     3601 aactcgaggg caaacgagcc gtcatagctt tctattggaa aaacttcctg cagcagctcc
     3661 tgcaatccct gccgggtccg catgtccgct tccctgctct cctgataaaa ccagttgtag
     3721 gaatcacgct gcacttcgat catgtcgggg atttcaacta aatcccgggc acgtccaaag
     3781 gtcaacctct ggcgcttgct gctgacgggg acaaattcgg ccatgaacct tttgacctcc
     3841 ctgaaataaa aatggggaaa atggtgctga caaagagtta agataacaaa aaggtcgtac
     3901 tatgtcaaca gacatagtac gaccttcgaa aaaaggttgc cctcaaattt tcgatggtgc
     3961 cgaagcgggg actcgaaccc cgacaggcgt tatgcctact agaacctgaa tctagcgcgt
     4021 ctaccaattc cgccaccccg gcctttcaaa accacgcggg taattgtagc aagggaagaa
     4081 ggcttttgca agagcgaaaa aagaattcct cggaaaaaat cactctttca ttgaagcccg
     4141 catcaatctg tcccgcagcg cgcgtcctat cccttcttcc gagggaggcc actgcgccgc
     4201 gataagaccg gctcccctct cctcaaagac cctgagcgca tggaaaaggc cgcgcgcgta
     4261 gttctccggg gagtcgaaga ggatacgcag cccggggtgc gcaacaccct cacccagact
     4321 gatctccggt gcatgcatgc cgatccacgc gatgcgcagc gctccgtccg tttccgccgc
     4381 ggcgcgagcg attccgtccg cgtccgtatc cagaagtagc ggcacgcggg gggcgtagtg
     4441 acgatacctc gttcccggcg acctgtgcgc cgcgccgccg gagaggagca ccttcatgcc
     4501 ggagcgggct tctatctcct cgaccgcgca ccctccggga cgaaggagaa cgagctgctc
     4561 gccggtggcg tcgaggacgg t
//
LOCUS       DAOTIL010000101         4529 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminobacteriaceae bacterium isolate AMR_MDS_3026
            NODE_22716_length_4529_cov_3.291909, whole genome shotgun sequence.
ACCESSION   DAOTIL010000101 DAOTIL010000000
VERSION     DAOTIL010000101.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868050
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminobacteriaceae bacterium (wastewater metagenome)
  ORGANISM  Aminobacteriaceae bacterium
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae.
REFERENCE   1  (bases 1 to 4529)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4529)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:19:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,224
            CDSs (total)                      :: 3,198
            Genes (coding)                    :: 3,184
            CDSs (with protein)               :: 3,184
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4529
                     /organism="Aminobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22716_length_4529_cov_3.291909"
                     /isolate="AMR_MDS_3026"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:3034609"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..700
                     /locus_tag="P5201_03210"
     CDS             <1..700
                     /locus_tag="P5201_03210"
                     /inference="COORDINATES: protein motif:HMM:NF013116.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="transaldolase family protein"
                     /protein_id="HRV97572.1"
                     /translation="KNVMIKIPATSAGMKAIRRCISLGVNVNATLIFSRAQYRQVAEA
                     WLSGLEERAAAGESVKGIASVASLFVSRVDTAVDKLLETTGEKDLAGKIAVDNARAAY
                     SDFEAIAASARWKTLAEKGALLQRPLWASTGTKNRAYSPTLYVDSLIGPHTVNTLPPA
                     TLEAFLESGEAALTVTSDKEGRSERLARLASCGVNLESVTDTLLQEGLVAFEKAYRVL
                     LEEIGKKGNLLSSK"
     gene            752..827
                     /locus_tag="P5201_03215"
     tRNA            752..827
                     /locus_tag="P5201_03215"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:785..787,aa:Ala,seq:ggc)
     gene            916..1329
                     /gene="speD"
                     /locus_tag="P5201_03220"
     CDS             916..1329
                     /gene="speD"
                     /locus_tag="P5201_03220"
                     /EC_number="4.1.1.50"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014163526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004014 - adenosylmethionine decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0008295 - spermidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylmethionine decarboxylase"
                     /protein_id="HRV97573.1"
                     /translation="MSPKGFHFIVEASGCGPIISQVDRLQEILVEAAKRANTQVWSVS
                     FHRFPPDGVSGVVVISESHLSIHTWPEVGYMALDIYTCGEESKPQIAVDYVLEQIGAK
                     HTHITEITRGLDDGDMEYYHSFVTWQEVLQRNSNN"
     gene            1572..2777
                     /locus_tag="P5201_03225"
     CDS             1572..2777
                     /locus_tag="P5201_03225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015556960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004180 - carboxypeptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HRV97574.1"
                     /translation="MNEKIRSLTKKYEEKMLELRRRIHKHPELSGEERETAALVASVL
                     EESGVEVQSDVGGHGVVGVLRGSAKGPCVALRADMDALPMEEKTGLPWASEVKGIMHA
                     CGHDSHTAMLLGAMLVLAEMKNELPGTVKCIFQPAEEANPVGGAPAMIQQGALKAPEV
                     DAIFGMHVWPSLVTGTIGTRSGALMGASDRLFLTVRGKGAHGSEPENGVDAIAIAAQI
                     LTALQSIVSRSVSPLDAAVVSVGTIKGGNRYNVIADEVLLEGTVRTINPETQEKMPGR
                     IEALVRGIARGMGGDCDMEYVKGYPPLMNDPELAKTAFSVLKKVVGEEKIVYVEKPAL
                     GGEDFAFFSKEIPALFLWLGCRPEGVAREDMPPLHNNMFLPDEKALSLGVETLVSCAL
                     EYLEGRKGA"
     gene            2774..4063
                     /locus_tag="P5201_03230"
     CDS             2774..4063
                     /locus_tag="P5201_03230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015556058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004180 - carboxypeptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HRV97575.1"
                     /translation="MKTTMDSSMKQRLVQLRRAFHAHAETGWTEFWTTAKIAGILKNL
                     GYQVSVGKEILDLESVMGRPSEDEMAKYIERAVHQGADPEWIKRMDGYTGAVAVLDTG
                     KKGPTLAFRFDIDAVDVAEAAEENHRPFREGFASMNENAMHSCGHDAHTVIGLGFAEL
                     LMRRKEELKGVFKLIFQPAEEGVRGGKAVAMKGILDDVDYFLGAHIGMGIPTGNVAPG
                     CGDFLCTTKFDAVYKGKAAHAGGAPNEGKNALLAAASATLALAGISPHKDGATRLNVG
                     VLHAGTGRNVVPSKAVMKVETRGLTTALNEYVYGRAMEIVEGAAVMYGVELSVAKMGE
                     AVDAFSDKELVDLVAEKALSVPGIEKLAPSVSLGGSEDVSWMMRAVQQHGGKAVYFVL
                     GSDIAAGHHNEFFDVDERVLSYGAALFADLAFALSER"
     gene            4175..>4529
                     /locus_tag="P5201_03235"
     CDS             4175..>4529
                     /locus_tag="P5201_03235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008523223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015558 - secondary active
                     p-aminobenzoyl-glutamate transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1902604 - p-aminobenzoyl-glutamate
                     transmembrane transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AbgT family transporter"
                     /protein_id="HRV97576.1"
                     /translation="MENNQQPRKRGIFESFIKGIEIVGNWLPHPFWLFVLLSLIVVGL
                     SYYLGNEGVAVKYMAAKAGEAPKEVTVAVENLLSFKYMRGFMADFVKTYVNFAPLGLI
                     VVMTLGIGLVERSGMI"
BASE COUNT         1093 a         1060 c         1322 g         1054 t
ORIGIN      
        1 gaaaaacgtc atgatcaaga taccggcgac atctgccggc atgaaagcca ttcgtcgctg
       61 tatctctctg ggagtgaacg tcaacgcaac gctcattttt tcccgggcgc agtaccgaca
      121 ggttgcagaa gcatggcttt cgggtcttga agaaagagct gcggcaggcg aatcggtaaa
      181 gggcatcgcc tcggttgcct ctctttttgt cagtcgagtt gacaccgcgg tggacaagct
      241 tctcgaaaca acgggagaaa aggatcttgc cggtaaaatc gcagtcgaca acgcccgtgc
      301 cgcatattcc gactttgaag cgatagcggc atccgcacga tggaaaactc ttgcggaaaa
      361 gggtgctttg cttcagcgcc cattatgggc cagcacggga acgaaaaacc gcgcctactc
      421 cccgacgctc tacgttgact ctcttatagg tcctcatact gtgaatacgc tgcctccggc
      481 gacccttgaa gcgttccttg agagcggaga ggccgcgctg acggttactt ccgacaagga
      541 aggacgatcg gaacgattgg ctcgtcttgc ctcatgcggt gtcaatctgg aatccgtaac
      601 ggataccttg ctccaagaag ggcttgtcgc ttttgaaaaa gcgtacagag ttcttctgga
      661 ggagatcggg aaaaaaggaa atcttctttc ttccaaataa gctgttgaca tattaggcgc
      721 cgctccttat aatactcccg tttgcgcata cggggctata gctcagttgg gagagcgctt
      781 gaatggcatt caagaggtca ggggttcgaa tccccttagc tccaccattt acgcaggatc
      841 aggccgtccg gtgagatcgg acggccattt ttcttccctt tgattacaaa tacactcaga
      901 aagaggagga aaacaatgtc accgaaaggg ttccatttca ttgtggaagc atcagggtgc
      961 ggaccgatta tttctcaggt ggacaggctg caggagatac ttgtcgaagc cgcaaaacga
     1021 gcgaatactc aggtctggtc ggtctccttt catcgctttc ctcccgacgg cgtgagcggt
     1081 gtcgtcgtca tcagcgaatc acatctctct attcatacat ggcctgaagt cggctacatg
     1141 gcgctggata tttacacgtg cggcgaggaa agcaagccgc agatcgccgt tgattatgtg
     1201 cttgaacaga taggcgcgaa gcatacgcat attacggaga ttacacgcgg cctcgacgac
     1261 ggcgatatgg agtattatca ttcctttgtt acctggcaag aggttcttca gcgcaacagc
     1321 aacaactaat agaaagaccc cgtggaaacc ttcaggtttg tacggggtct ttttctgtat
     1381 acccgcctcg cgcatcatgc atcgttgaaa atatccggcg tctatgttac cttatcacgg
     1441 aagtctttca aaattgtcca ttagcggttc aacatccggg attgcgctga aaaccgctgt
     1501 cagctgtctt tcgcacgtta aacagccttc cgtgaaaaaa gcagaaatga attcaacagg
     1561 aggaaccccc agtgaacgag aaaatacgtt cgttaacaaa aaaatacgaa gagaagatgc
     1621 tggagctccg ccgccgcatt cacaaacatc ctgaattgag cggtgaggag cgcgaaactg
     1681 cggcgctggt agcgtccgtt cttgaagaat ctggagtcga agtgcaatcc gatgtcgggg
     1741 ggcatggcgt tgtcggtgtg cttcgaggga gcgcaaaagg tccctgcgtc gctcttaggg
     1801 cggatatgga cgccctccca atggaggaga aaacagggct tccctgggcg tcggaagtaa
     1861 aaggaatcat gcacgcgtgc gggcatgatt cgcacaccgc aatgcttttg ggtgcgatgc
     1921 ttgtacttgc cgagatgaaa aatgagcttc ccggtacagt caagtgtatc tttcagcccg
     1981 ctgaggaggc aaatccggtc ggaggggcgc ctgcgatgat ccagcaagga gccctcaaag
     2041 cgcctgaggt cgacgctatt ttcggcatgc acgtgtggcc ctctcttgtt acaggcacga
     2101 tcgggacgcg ttcaggggct cttatgggag cttccgatcg cctctttttg actgtgcgag
     2161 ggaagggcgc ccatggctcg gaacctgaaa acggagttga cgccattgcg atagcggcgc
     2221 agatactcac cgcgctgcag tcgatcgtct cgcgcagcgt gagtcctctt gatgcggcag
     2281 tcgtaagcgt gggtacaatc aaaggcggaa acaggtacaa tgttatagcc gatgaagtgc
     2341 tccttgaggg aacagtgcgg acgatcaacc ctgaaacgca agagaagatg cccggtcgta
     2401 tagaagcgct cgtccgtggt atcgcaagag gtatgggcgg ggattgcgac atggagtacg
     2461 tcaagggata tcctcccctc atgaatgatc cagagcttgc aaagaccgct ttttctgttt
     2521 tgaagaaggt tgtcggggaa gaaaagattg tgtacgtaga aaagccggct cttggaggag
     2581 aagatttcgc tttcttcagc aaagagatcc ccgctctttt tctttggctt ggatgcaggc
     2641 ctgagggagt agccagagag gatatgcctc ctcttcataa caacatgttt cttccggatg
     2701 aaaaagcgct ttctctaggg gtggaaacgc ttgtttcctg cgctcttgaa tatctggaag
     2761 gacgtaaagg agcgtgaaga cgacgatgga cagttcgatg aagcaacgac tcgttcaatt
     2821 gagaagagcg tttcatgcac atgccgagac tggctggacg gagttctgga cgacggcgaa
     2881 aattgcagga attttaaaga acctcgggta tcaggtttcc gttggtaagg aaattctcga
     2941 tttggaatcc gttatgggac gcccgtcgga agatgagatg gcgaaataca ttgagcgtgc
     3001 agtacaccaa ggagccgacc cggagtggat taaacggatg gacggatata caggagcagt
     3061 cgccgtgcta gataccggga aaaaaggccc gacgctcgct tttcgtttcg atatcgatgc
     3121 ggtggatgtt gcggaggctg ccgaagagaa tcatcgtccc ttccgcgagg ggtttgcatc
     3181 catgaacgag aacgctatgc attcgtgcgg gcatgatgcg catacggtga ttggtcttgg
     3241 ttttgcagaa cttctcatga ggcgtaaaga agaactcaag ggagttttta aattaatatt
     3301 ccagcctgct gaagagggcg tccgcggagg aaaagcggta gcgatgaagg gcattctgga
     3361 cgatgttgac tacttcctcg gcgcgcatat cggtatgggc attccaacgg gaaatgttgc
     3421 tcctggatgc ggcgattttc tttgcacaac gaagttcgac gccgtctaca aggggaaagc
     3481 cgcgcatgcc ggaggcgcgc cgaacgaggg aaaaaacgct cttctggcgg cggcgtccgc
     3541 aactttggct cttgccggga tttcgccgca caaagatgga gcaacccgcc tcaacgtcgg
     3601 tgttctgcat gcggggacag gccgcaatgt cgtcccttcg aaggcggtaa tgaaagtgga
     3661 aactcgcggt ctgactaccg cgttgaacga gtatgtgtac ggtcgagcga tggaaatagt
     3721 cgagggtgcg gcagtcatgt acggtgtgga actgtcggtt gccaagatgg gagaggctgt
     3781 cgacgcattc agcgacaaag agctcgttga tcttgtggcg gaaaaggcgc tttccgttcc
     3841 gggtattgag aaacttgccc cctccgtcag tcttggcggc agcgaggatg tttcctggat
     3901 gatgcgcgcc gtacagcagc atggcgggaa agccgtctat tttgtacttg gctccgatat
     3961 tgcagcgggg caccacaacg agttttttga cgtggacgaa agggtcctga gttacggggc
     4021 ggcccttttc gccgatttgg cgtttgcctt gtcggagaga taagttccta aggagaggag
     4081 gggcgtgagg aagtattctg aaacacccgg ttatacgcta atgggtagaa aaacaggaga
     4141 tcgttgcaaa ttttgatacc aaggagggat attgatggaa aacaatcagc aacctaggaa
     4201 aaggggtatt tttgaaagct tcatcaaagg gattgaaatc gtcggcaact ggctgcctca
     4261 tccgttctgg ctctttgttc ttctttcgct catagtggtt ggtctctcat actacctcgg
     4321 caacgaaggt gtcgctgtaa agtacatggc ggcgaaggcg ggagaggcgc cgaaagaagt
     4381 cacggttgca gttgagaatc tgctttcatt caagtacatg cgaggtttca tggcagattt
     4441 cgtcaagacg tatgtgaatt ttgcccccct tggacttatc gtcgtcatga cgctgggcat
     4501 cggtcttgtc gagcggtccg gaatgatct
//
LOCUS       DAOTIL010000102         4525 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminobacteriaceae bacterium isolate AMR_MDS_3026
            NODE_22738_length_4525_cov_2.932438, whole genome shotgun sequence.
ACCESSION   DAOTIL010000102 DAOTIL010000000
VERSION     DAOTIL010000102.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868050
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminobacteriaceae bacterium (wastewater metagenome)
  ORGANISM  Aminobacteriaceae bacterium
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae.
REFERENCE   1  (bases 1 to 4525)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4525)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:19:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,224
            CDSs (total)                      :: 3,198
            Genes (coding)                    :: 3,184
            CDSs (with protein)               :: 3,184
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4525
                     /organism="Aminobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22738_length_4525_cov_2.932438"
                     /isolate="AMR_MDS_3026"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:3034609"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..490)
                     /locus_tag="P5201_03240"
     CDS             complement(<1..490)
                     /locus_tag="P5201_03240"
                     /EC_number="3.5.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012869167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016812 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds, in cyclic amides
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclase family protein"
                     /protein_id="HRV97577.1"
                     /translation="MSNSLELKNWSNIKVYDLTIPIGIQTPPWPTYEPLQVKYFKRLA
                     PNGANGQLLTHSNHVGTHLDGPIHFCGHGGDIASLPLKDFLVGPGVVVDIQDIAEDYG
                     IYTPEDLEARADIREGDILIINTGYHRYGWNEPEADEVRYMVKHPGPTREFATWALKK
                     KIK"
     gene            complement(531..>1807)
                     /locus_tag="P5201_03245"
     CDS             complement(531..>1807)
                     /locus_tag="P5201_03245"
                     /inference="COORDINATES: protein motif:HMM:NF018275.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF1116 domain-containing protein"
                     /protein_id="HRV97578.1"
                     /translation="GGQLNVIDLEKANQEALSRLLAAQPLIVGMGIAKETVPGMKDKM
                     LLHAGPPLTWETASGPIRGGVMAACMYEGWAKTPEEAEKLAASGAIAFDPCHHHHAVG
                     PMAGIMSPSMPVFVLENRTHGNKAYVTMNEGLGKVLRMGAFGPEVIEHLQWMEKTLYP
                     VLKAAIEHAVKKGEEPDVKNMIAQALHMGDELHNRNKAGTSLFLRTLAPHMVETCKDS
                     AQLAEVFRFIDKTDHFFLNIAMAAGKASLEAAAGIEGSSMLTVMARNGTEFGLQVSGL
                     GDRWFTCEAALPDVLLFPGFTREDTNRDIGDSAIMETYGVGGFALAAAPAIVQFIGGT
                     PKDAANYTLEMYEITAGENSMFTIPALNFRGTPTGIDVLKVVETGITPVLDTGAAHKE
                     PGKGQVGAGIVRMPMDAFVKAAEAFADKYLSE"
     assembly_gap    1808..1907
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(1948..2118)
                     /locus_tag="P5201_03250"
     CDS             complement(1948..2118)
                     /locus_tag="P5201_03250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009202420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRV97579.1"
                     /translation="MKKLLKEGPKVVNIGVEQFFLDVKTQKTEAVVWDWKPPAASGDL
                     LSRLKKLKKGGN"
     gene            complement(2115..3674)
                     /gene="fdrA"
                     /locus_tag="P5201_03255"
     CDS             complement(2115..3674)
                     /gene="fdrA"
                     /locus_tag="P5201_03255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012869164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA synthetase FdrA"
                     /protein_id="HRV97580.1"
                     /translation="MASSQRLEIIRRTYYDSVTLMLVAKELLKLEGISVATLNMGTEA
                     NYKIMGAGGFDLTGVDATPNDLVVALLSDAPEAEKVLDDALAKAKEYLANPPGRKKDS
                     GDNDYRPKSLDGALSVLPEANLAIVSVAGRYAGDVALQCIEKGLNVMLYSDNVTVEKE
                     IEVKKAAAAKRLLVMGPDCGTIIIRGVAMGMANACPTGPVSIVAAAGTGLQEVHVQLA
                     RRGVGVLHGIGTGGRDVKKEVGGIMCLQAVEALLADDEVKVLAVLGKPPAPEVEKKIL
                     DAVKAGGKPAVLGFIGGEAKGDSDGVFVCRELEETAAVAAALANGQDAAAARDALYAE
                     YDALKQQAEKIGKRKGYLRGLYSGGTLCYEGQLVARGILGPIWSNTPLDASKKLADSL
                     KSVEHTMVDYGEDEFTQGRLHPMIDVSLRADRILEEAKDPGVGVILLDVVLGYGCNDD
                     PATGLAEGIRKARKVAGERIAFVASICGVDTDPQNASRQRKILEELGVHVCESNARAA
                     RLAAMLVEVAQ"
     gene            complement(3690..4295)
                     /locus_tag="P5201_03260"
     CDS             complement(3690..4295)
                     /locus_tag="P5201_03260"
                     /inference="COORDINATES: protein motif:HMM:NF022834.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2877 domain-containing protein"
                     /protein_id="HRV97581.1"
                     /translation="MNLEEPESGRRYSVVTSGEDFGPRTWLTDLLPPLRQGELLSLPG
                     EGIPARYDPSLPAGRMNLRWVPLVCEWSSLLEEELESLAAPARERRFGDLLGLGPGST
                     PAGDDFLAGYTTGRLWLGAPVSPLPDTSRTTWLSAEILRDAADGLVWKRSKDVLESLS
                     LNDAAALLKAVERILGWGHTSGRAWLAGLSAAVSDTIERNV"
BASE COUNT          728 a         1460 c         1330 g          907 t
ORIGIN      
        1 acttgatctt cttcttgagc gcccaggtgg cgaactctct ggtgggtccg gggtgcttca
       61 ccatgtagcg gacttcgtcg gcctcgggct cgttccagcc gtaccggtgg tacccggtgt
      121 tgatgatgag gatatcgccc tcgcggatgt cggcgcgggc ttcaaggtct tcgggagtgt
      181 agatgccgta gtcctcggcg atatcctgga tgtcgaccac aacgccgggg ccgacaagga
      241 agtccttcag cggaaggctg gcgatatctc cgccgtggcc gcagaagtgg atcgggccgt
      301 cgaggtgcgt tcccacgtgg ttggagtggg tgagcagctg gccgttcgct ccgttgggag
      361 cgaggcgctt gaagtacttc acctgaagcg gctcgtaggt gggccatggc ggcgtctgga
      421 tgccgatggg aatggtcaga tcgtacacct tgatattgct ccagttcttg agttcgagac
      481 tgttggacat agtaagtacc tccttgtgtt ttagtaaact tggataagaa ttactcgctc
      541 aaatacttgt ccgcgaaggc ctccgcggcc ttcacgaagg cgtccatcgg catgcggacg
      601 atacccgcgc cgacctgccc cttgccgggc tccttgtgcg ccgcacctgt atcgagcacc
      661 ggggtgattc ccgtctccac aaccttgagg acgtcgatcc cggtcggggt gccgcggaaa
      721 ttcaacgcgg ggatggtgaa catgctgttc tctccagcgg taatctcgta catttcaagc
      781 gtgtagttcg ccgcgtcctt cggcgtgccg ccgatgaact ggacgatggc cggggccgcg
      841 gcgagcgcga agccgcctac gccgtaggtc tccatgatgg cgctgtcgcc gatgtcgcgg
      901 tttgtgtcct cgcgggtgaa cccggggaag aggagcacat ccggcagggc cgcttcgcag
      961 gtgaaccaac ggtcgccgag gccggagacc tggaggccga actccgtgcc gttgcgcgcc
     1021 atgacggtga gcatgctcga accttcgatc cctgcggccg cttcgagaga ggcctttccc
     1081 gccgccatcg cgatgttcag gaagaagtgg tcggtcttgt cgatgaaacg gaagacctcg
     1141 gcgagctgtg cgctgtcttt gcacgtctcc accatgtgcg gcgcgagcgt gcggaggaac
     1201 aacgacgtcc ccgccttgtt gcggttgtgc agctcgtcgc ccatgtgaag cgcctgagcg
     1261 atcatgttct tgacgtccgg ctcctcgccc ttcttcaccg cgtgctcgat tgccgccttg
     1321 agcacagggt agagcgtctt ctccatccac tgcagatgtt cgatgacctc ggggccgaag
     1381 gcgcccatac gaagcacctt cccgagtccc tcgttcatcg tcacgtacgc cttgttgccg
     1441 tgcgtcctgt tctccaggac gaatacgggc atgctcggcg acatgatccc cgccatcggc
     1501 cccaccgcgt ggtggtgatg gcacggatcg aaggcgatgg cgccgctcgc ggcgagtttt
     1561 tccgcctctt caggcgtctt cgcccaccct tcgtacatgc aggccgccat gacgcccccg
     1621 cggatgggac cggacgcggt ctcccacgtc aggggaggcc ccgcatgcag gagcatcttg
     1681 tccttcatcc ccggaacggt ctctttcgct atccccatgc cgactatcag cggctgggcg
     1741 gcgagcaggc gcgagagcgc ctcctgattt gctttctcca gatcgatcac gttcagttgc
     1801 cccccttnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1861 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnngag agcgcctcct
     1921 gatttgcttt ctccagatcg atcacgttca gttgcccccc ttcttgagct tctttaaacg
     1981 agagaggaga tccccgctcg ctgcgggagg cttccagtcc cagacgaccg cctccgtttt
     2041 ctgcgttttt acgtcgagga agaactgctc caccccgata ttgaccactt tcgggccttc
     2101 tttcagcagc tttttcattg cgctacctcc acgagcatcg ccgcaaggcg ggccgctctg
     2161 gcgttgcttt cgcaaacgtg cacgccgagc tcctccagta ttttccgctg ccgggacgca
     2221 ttctgggggt cggtatccac gccgcagatc gaggcaacga aggcgattct ctctcccgcg
     2281 accttccgcg ccttgcggat tccctcggca agccccgtcg ccgggtcgtc gttgcagccg
     2341 tagccaagca ccacgtcgag cagaatgacg cccactccgg gatccttcgc ctcttcgagg
     2401 atgcggtcgg cgcggagcga cacgtcgatc atcggatgca gccggccctg ggtgaactcg
     2461 tcctcgccgt agtcgaccat ggtgtgctcc acgctcttga ggctgtcggc gagcttcttc
     2521 gacgcgtcaa gcggcgtgtt gctccatatc gggccgagga tcccgcgcgc gacgagctgc
     2581 ccctcgtaac agagagtgcc gccggagtag agtccgcgga ggtagccctt ccgcttcccg
     2641 atcttctccg cctgctgttt cagcgcgtcg tactccgcgt agagcgcgtc gcgggccgcc
     2701 gccgcgtcct gtccgttcgc gagcgcggca gcgacggcgg cggtctcctc aagttcgcgg
     2761 cagacgaaca cgccgtccga atcgcctttc gcctcgccgc cgatgaaacc aagaactgcg
     2821 ggcttgcctc ctgccttcac cgcgtcgaga attttcttct cgacctcggg cgcgggcggc
     2881 ttgccgagca cggcgagcac cttcacctcg tcgtcggcga gcaacgcctc caccgcctgc
     2941 aggcacatga tgccgccgac ctcttttttc acatctcgcc cgccggtacc gatgccgtga
     3001 aggacgccga cgcccctgcg ggcaagctgc acatgcactt cctgcaaccc cgtcccagcc
     3061 gcggccacaa tgctcacggg cccggtcgga caggcgttcg ccatccccat cgcaacgccg
     3121 cggataatga tcgtgccgca gtcgggcccc atcacgagca gtctcttcgc ggcggccgcc
     3181 ttcttgacct cgatttcctt ctcgaccgtg acgttgtcgg agtagagcat cacgttgagc
     3241 cccttctcga tgcactgaag ggcaacgtcg cccgcgtagc gcccggcgac cgagacgatc
     3301 gcgagattgg cttcaggaag gaccgagagc gccccgtcga ggctcttcgg cctgtagtcg
     3361 ttgtcgcccg agtccttctt ccgccccgga gggttcgcaa ggtactcttt cgccttcgcg
     3421 agagcgtcgt caaggacctt ttcggcctcc ggcgcgtcgc tcaaaagggc gacgacgagg
     3481 tcgttcggcg tggcgtccac tccggtcagg tcgaaaccgc ccgctcccat aatcttgtag
     3541 ttcgcctccg tccccatgtt cagagtggcg acggagattc cctccagctt gagcagctcc
     3601 ttcgccacaa gcatcagggt gacgctgtca tagtacgttc ttcggatgat ttcgaggcgc
     3661 tgggaagatg ccacagtatc atcctccttt catacgttgc gttcgatcgt gtccgagacg
     3721 gccgcggaaa gccccgcgag ccacgctctt ccggaagtgt gcccccagcc gaggattcgt
     3781 tcgaccgctt tcagaagcgc agccgcgtcg ttcagcgaaa gagattccag tacatctttg
     3841 cttcgtttcc agaccaatcc gtccgcagcg tccctcagaa tttcggcgga gagccaggtc
     3901 gtccgggacg tatccggcag aggggaaaca ggagctccga gccagagtct cccggttgtg
     3961 tatccggcaa ggaagtcgtc cccggcgggc gtgctccccg gcccgaggcc gagaagatcg
     4021 ccgaagcgcc gttcccgggc cggtgcagcg agagattcca gctcctcttc gagcagcgaa
     4081 ctccactcgc aaaccagcgg aacccatcga aggttcatac gtccggcggg aagcgacggg
     4141 tcgtaccgcg cagggattcc ctcccccggt agagagagaa gctctccctg ccgcagaggg
     4201 ggaagaaggt ccgtcagcca cgtcctcggg ccgaaatcct cccctgacgt gacaacggaa
     4261 tacctccgcc cgctctcagg ttcttcaagg ttcactgttc gcgtatatat ttcccgcacc
     4321 cgcagcaagg tgaattcacc gccccgaaag gggcgatgaa gagaaggtcc cctgggggac
     4381 gccggaagga gaccctcttt ccgattcgca gtccgggaga gcacatcgcc gctgcaggcg
     4441 cggagctgca tgctctttca accgccctgt attgccggag gctacaccac gccgtccttt
     4501 ttgagctcct caaactcggc ggcgg
//
LOCUS       DAOTIL010000103         4519 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminobacteriaceae bacterium isolate AMR_MDS_3026
            NODE_22788_length_4519_cov_2.741039, whole genome shotgun sequence.
ACCESSION   DAOTIL010000103 DAOTIL010000000
VERSION     DAOTIL010000103.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868050
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminobacteriaceae bacterium (wastewater metagenome)
  ORGANISM  Aminobacteriaceae bacterium
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae.
REFERENCE   1  (bases 1 to 4519)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4519)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:19:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,224
            CDSs (total)                      :: 3,198
            Genes (coding)                    :: 3,184
            CDSs (with protein)               :: 3,184
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4519
                     /organism="Aminobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22788_length_4519_cov_2.741039"
                     /isolate="AMR_MDS_3026"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:3034609"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1015)
                     /locus_tag="P5201_03265"
     CDS             complement(<1..1015)
                     /locus_tag="P5201_03265"
                     /inference="COORDINATES: protein motif:HMM:NF012861.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ClC family H(+)/Cl(-) exchange transporter"
                     /protein_id="HRV97582.1"
                     /translation="MRRYALLTAGSRFRAHIVFQGIVVGLSAGVASICYRLLLEYAEE
                     FSFRALRGGYSLPLPALLLLWTAVALFIGKLMEKEPMARGSGIPQVEGELMGCVSMNW
                     LRVLWAKFLGGGLAIALGLSLGREGPSVQIGAAVGKGLSRLQKKTKMEEKLLLSSGAS
                     AGLAAAFNAPLSGVIFALEEVHKSFSPLILLSAMMASLGADFLSKTFFGLGPVFSFGV
                     PAPLPLGFYGHFLVLGVLCGVGGALFCRAILELQTLYAKLPIPASCRVVIPALLAVVA
                     GSFLPEILGGGHALVEYLGHGSHPASFILLLFVGKLLFTALCFGSGAAGGIFLPMLAV
                     GASL"
     gene            complement(1028..2164)
                     /locus_tag="P5201_03270"
     CDS             complement(1028..2164)
                     /locus_tag="P5201_03270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005659380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HRV97583.1"
                     /translation="MLYRTMPQTGDKISILGFGCMRFPLDAEGKIDEPRSAAMLHSAI
                     DRGVNYIDTAYPYHGGESEPVVGRALEGGYREKVFLATKLPSWMVETPSDMDRLLDEQ
                     LTKLRTDHIDYYLLHSLNADRWGIMKRNDYGKFLERALADGRIRRAGFSFHDQLPLFK
                     EIVDDWPWHFCQIQYNFLDTNYQAGTEGLKYAAEKNIGMVIMEPLRGGNLAKNVPDAM
                     MRIWNESPKKLSPAGWALRWIWDQPEVNVVLSGMTTESDLDDNLSAAEEGAPDSLTVA
                     EREMISRVTTEYRTRMKVGCTACQYCMPCPAGVNIPECFNRYNMAFMFDNLEQAKATY
                     PVFLKPGARASACVKCGACEEKCPQNLPIREHLASVVELLEPGE"
     gene            complement(2386..3477)
                     /locus_tag="P5201_03275"
     CDS             complement(2386..3477)
                     /locus_tag="P5201_03275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005660638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class III-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HRV97584.1"
                     /translation="MLSPVYAPYSFVVERAEGVRLFTDKGVFLDTYAGIGVLPLGHSH
                     PDVVRAIREKSERYTHLSNYFLDPDAPALAESLLFMTGRKGEVYFANSGAEAMEAALK
                     AVKKNREGALVSFEGNFHGRTLGALSVTWGPAIRHPFDPLIPHCAFLPFNGEALLRFA
                     RENEIAAVFLECIQGNSGVYPLPADLAEAVNILQKERGILVVADEIQAGLGRTGRYFA
                     YEHFGLRPDIITLGKGIGGGLPLGAAIFCEWSPFASGEHGSTFAPNPVSLAAGKATLA
                     HITPSLLGDVSRKGERLRERLALLSWARGTRGLGLMIGVSAENPAEVKKKAFERGVLL
                     NVTGGGIRFLPALTMSDAEIEEILSLLNF"
     gene            complement(3482..>4519)
                     /locus_tag="P5201_03280"
     CDS             complement(3482..>4519)
                     /locus_tag="P5201_03280"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013047429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004072 - aspartate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate kinase"
                     /protein_id="HRV97585.1"
                     /translation="EPSPRELDMLLATGEQMTSALLAMALSDFGIPALSYNAFQLEMT
                     TTGDFSNARIMDLNLNKLKRQLEMRSVIVVTGFQGITPDGDLTTLGRGGSDTSAVAIA
                     AKCGCPCEIYSDVAGVYTCDPNKRAGARKLEYITFDEMLELSSLGAKVLHSRAVEIAK
                     KYSVDLYCASTFSDERGTYVVNSLPEWLEQPVVTGVTTDSNQMRVNIVRMPASMDAVS
                     ALFGGLAEQGVNVDMISTVNENGHSHLTFTVVDAKEKTVLETVRRTLAPWEGWNVTED
                     RNVAKISAVGVGMKSAPGVAARFFGALSRKGIKMLGTTTSEIKISALVSREEGDEALR
                     ALVDEFGLGEA"
BASE COUNT          919 a         1453 c         1332 g          815 t
ORIGIN      
        1 ccagcgacgc gccgacagcg agcatgggga gaaagatgcc tcccgccgct cccgagccga
       61 aacagagtgc ggtgaagagg agtttcccta caaagagcaa cagaatgaag ctcgcaggat
      121 gactcccgtg gccgagatac tccacgagcg catgccctcc tccgaggatt tcggggagaa
      181 aagagcccgc aacgacggca aggagggcgg ggatgacaac gcggcaggac gcaggaatcg
      241 ggagtttcgc gtagagcgtc tgcagttcca gaatagcacg gcaaaagagc gcgcccccga
      301 cgccgcagag cacgccaagc acgagaaagt gtccgtagaa cccgagcggc aggggggcgg
      361 gaacgccgaa ggaaaacacc ggccccagcc cgaaaaatgt ctttgacaga aagtcagcgc
      421 cgagcgaagc catcatcgcc gacagcaaaa tcagcgggga gaagctcttg tgcacctctt
      481 caagggcgaa gatcaccccc gagagcggcg cgttgaacgc agcggcgagt cccgccgacg
      541 cgccgctgga gagcagcagc ttctcctcca tcttcgtctt tttctgaagg cgggagagcc
      601 ctttcccgac cgcagcgccg atctgcaccg acggcccttc acgtccaagc gaaagcccga
      661 gggcgatcgc cagaccgccg ccgagaaact tcgcccacag cactcgcagc cagttcatcg
      721 agacgcaccc cataagttcg ccctcgacct gaggaattcc gcttccccgc gccatcggct
      781 ctttttccat gagcttgccg atgaagagcg cgacagctgt ccacaggagc agaagcgcgg
      841 gaaggggaag cgaatagccc cccctcaacg cacgaaaaga gaactcttcc gcatactcca
      901 ggagcaggcg gtagcagata gaggcgacgc ccgccgaaag tccgacgacg atcccctgga
      961 agacgatgtg cgcacggaag cggcttccgg ccgtcagcag agcgtaacgc cgcatcggaa
     1021 aacggcctta ctcgccgggt tccagcagct cgaccaccga cgcgagatgt tcgcggatcg
     1081 gaagattctg cgggcacttc tcctcgcagg cgccgcactt gacgcacgcg ctcgcccgcg
     1141 cccccggctt caggaatacc ggataggtcg ccttggcctg ctcaagatta tcgaacataa
     1201 aggccatatt atagcggttg aagcattcgg ggatattcac gcctgcggga cagggcatgc
     1261 agtactggca cgcggtgcat ccaaccttca tcctcgtccg gtactccgtc gtgacgcggg
     1321 agatcatctc gcgctctgct accgtcagag aatccggcgc gccctcttcc gcagctgaga
     1381 gattgtcgtc gaggtcgctc tccgttgtca tgccgctgag caccacgttg acctccggct
     1441 gatcccagat ccaccggagc gcccaccctg cgggagagag cttcttcggc gattcgttcc
     1501 agatgcgcat catcgcgtcg gggacgttct tcgccaggtt gcctcctctg agcggctcca
     1561 tgatcaccat gccgatgttc ttctccgcgg cgtacttcaa cccttccgtc ccggcctggt
     1621 agttcgtgtc gaggaagttg tactgaatct ggcagaagtg ccacggccag tcgtccacga
     1681 tctctttgaa gagcggcaac tggtcgtgga aggagaagcc cgcacgccgg atacgtccgt
     1741 cggcgagagc tctttcgagg aacttgccgt aatcgttccg tttcatgatt ccccaccggt
     1801 ccgcgttcag cgagtggagc aggtagtagt cgatatggtc cgtacgcagt tttgtcaact
     1861 gctcgtcaag aagacggtcc atgtccgacg gcgtctccac catccacgag ggaagcttgg
     1921 ttgcgaggaa aaccttctcg cggtatccgc cttcgagcgc ccggccgacc acgggctcgc
     1981 tctctcctcc gtgatacggg tacgccgtat cgatgtagtt cacgcccctg tcgatagcgg
     2041 aatgcagcat cgccgcagag cgcggctcgt cgatctttcc ttcggcatcg agaggaaagc
     2101 gcatacagcc aaaaccaaga atggaaatct tatcccctgt ctggggcatt gtacgataga
     2161 gcatattcaa tacaacctcc ttcgtatttc gccgcgcctg agagaagtgc cggcacgaaa
     2221 aacaggcgcg ccatctttcc gtatatcata tattatcgca tagactttcc tgtcgcgcag
     2281 tactccgaat aacaggaagg tccggaatcc ggccccaagc ggcacagatt ccgaacctct
     2341 ctgataaagg cggagtatgg gggaagcgac ttcccggcct cgtcctcaga aattcagcag
     2401 cgacaggatc tcttcgatct ccgcgtcgct cattgtgagc gcggggagga atcggatccc
     2461 gccgcccgtt acattgagca gcacgccgcg ctcgaaggcc tttttcttca cctccgcggg
     2521 attctccgcg ctcacgccga tcatgagccc cagcccgcgc gtcccgcgcg cccaggagag
     2581 aagcgccagc cgttcgcgga gtcgttcgcc cttccgggag acgtcgccca gcagagaggg
     2641 tgtgatgtgc gcgagcgtcg ctttgcctgc ggcgagcgag acagggttcg gggcgaaggt
     2701 cgagccgtgc tcgccggatg cgaacggcga ccactcgcaa aagatcgccg ctcccagggg
     2761 aagccccccg ccgatcccct tcccgagagt gatgatatcg gggcgaaggc cgaagtgctc
     2821 gtaagcgaag tacctgcccg tgcgcccaag gcccgcctga atctcgtcgg ccaccaccag
     2881 gatgccccgc tctttctgca ggatgttcac cgcctcggcc aggtccgcag gaagcggata
     2941 gacgccgctg ttcccctgga tacactcaag gaagacggca gcgatctcat tctcccgcgc
     3001 gaacctcagc agcgcctccc cgttgaacgg gagaaaggcg cagtgcggaa tgagcggatc
     3061 gaaaggatgg cgtatcgcag gcccccaggt cacggagagc gcacccaagg tacgcccgtg
     3121 gaagttgccc tcgaaagaga cgagcgctcc ttcgcggttc ttcttcaccg ctttcagagc
     3181 ggcttccatc gcttcggcgc cggagttggc aaaatacacc tcgccttttc tgccggtcat
     3241 gaagagcagc gattcggcaa gcgcaggcgc gtccgggtcg agaaagtagt tcgacagatg
     3301 cgtatagcgt tccgatttct cacggattgc ccggacgaca tccgggtggc tgtgtccgag
     3361 agggaggacg ccaatgcccg cgtacgtatc caggaagacg cccttgtccg tgaagagccg
     3421 gacgccttcc gcccgctcga caacaaaact gtacggcgca taaacggggg agagcatccg
     3481 gctatgcctc ccccaggccg aactcatcca ccagggcgcg cagcgcctcg tcgccctctt
     3541 cgcgggagac gagcgcagag attttaatct ccgacgtcgt agtccccagc atcttgatcc
     3601 ccttccggga gagcgcgccg aagaaacgcg ccgcgacgcc cggggccgac ttcatgccga
     3661 cgccgaccgc ggagatcttc gccacatttc ggtcttctgt gacgttccac ccctcccaag
     3721 gggcgagcgt cctccggacc gtttccagca cagtcttctc cttggcgtcc accacagtga
     3781 aggtgaggtg cgagtggccg ttttcattga cagtggagat catatccacg ttcactccct
     3841 gctcggcgag cccgccgaag agcgcgctca cggcatccat ggaggccggc atgcgtacga
     3901 tgttcacccg catctggttg ctgtccgtgg tcactccggt caccacaggc tgttccagcc
     3961 attcgggaag gctgttcacc acgtaggtcc ccctttcatc ggagaaggtc gacgcgcagt
     4021 acagatcgac cgagtatttt tttgctattt ccaccgcccg cgagtggagc accttcgccc
     4081 ccagcgagga gagctcgagc atctcgtcga aggtgatgta ctccagcttt ctcgccccgg
     4141 cgcgtttgtt cgggtcgcag gtgtacacac cggccacatc gctgtagatc tcgcaggggc
     4201 agccgcactt cgccgcgatg gcaaccgccg aggtgtcgga ccctccccgg ccaagcgttg
     4261 taagatcccc gtccggggtt atcccctgga aaccggtgac gacgatgacg ctccgcattt
     4321 cgagttgccg cttcagcttg ttcaggttca agtccatgat ccgggcgttg ctgaaatcgc
     4381 cggtcgtggt catctcaagc tggaacgcat tgtaggagag cgccggaata ccaaagtcgc
     4441 tcaacgccat cgccagaaga gcggaggtca tctgctctcc ggtggcgagg agcatgtcca
     4501 gctcacgggg cgacggctc
//
LOCUS       DAOTIL010000104         4509 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminobacteriaceae bacterium isolate AMR_MDS_3026
            NODE_22843_length_4509_cov_3.516390, whole genome shotgun sequence.
ACCESSION   DAOTIL010000104 DAOTIL010000000
VERSION     DAOTIL010000104.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868050
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminobacteriaceae bacterium (wastewater metagenome)
  ORGANISM  Aminobacteriaceae bacterium
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae.
REFERENCE   1  (bases 1 to 4509)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4509)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:19:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,224
            CDSs (total)                      :: 3,198
            Genes (coding)                    :: 3,184
            CDSs (with protein)               :: 3,184
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4509
                     /organism="Aminobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22843_length_4509_cov_3.516390"
                     /isolate="AMR_MDS_3026"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:3034609"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1517
                     /locus_tag="P5201_03285"
     CDS             <1..1517
                     /locus_tag="P5201_03285"
                     /inference="COORDINATES: protein motif:HMM:NF012688.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="DNA polymerase"
                     /protein_id="HRV97586.1"
                     /translation="PGAEAAPLLGRLGELSKGSVLFPDFKEAAACFGSSALSPERVRD
                     FKTAHYLLHPDKNAHLPEAVIPGYESAALAERGAAILAEYGRMERELAKYPGLSDLMR
                     TVDIPLIPVLVDMEQYGISCCSQTFGALEKDLSERLSGIEEEIASTAGERINLNSPKQ
                     VAWLLFEKLGLPAGKKTKTGYSTDISVLEGLSALGRPFDEVPNLLIEYRELSKMLSGF
                     VQPLTKAGEAGEGVIHGIFEPAVTGTGRLSSREPNLQNLPTFGEWSKRVKEGLQPHGE
                     DRMFVAADYSQIELRILAHLCGDKRLLDAFASGRDIHTETASWVFSTEPAFVTPELRR
                     VAKMINFGLLYGMSAFGLAQRLAIGRQEASDIVKRYFEALPGVKEYLDAGYIEAKERG
                     YTRTVAGRIRPLAEVSVSPRDRDALRRVAVNTPIQGSAADIARKAMIDFARRFPKEGS
                     VRLALQVHDSLVCECARDEAPAVESELVKIMEGAATLSVPLKVEAKKGGNLAEV"
     gene            1593..2699
                     /locus_tag="P5201_03290"
     CDS             1593..2699
                     /locus_tag="P5201_03290"
                     /inference="COORDINATES: protein motif:HMM:NF025010.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SurA N-terminal domain-containing protein"
                     /protein_id="HRV97587.1"
                     /translation="MRTLRSQVRWIMIAIVVLFVLSIFGMYGFDGARRGRPQSGESGD
                     YVVAEIDGKPVMRSALDQSVRNYVERMNVREIKPEDIPHLYQSTLESLVINHRLALEA
                     EQSGLKATDEELDAAVREVSEQFPTKEAFMQYLDQSGVKMAAFRENLATQIVQQKMVE
                     RASAGIAVTEEEALDFYEKTKVLFFHSPAGYTMDYARLKNEAAAAKIVEAVRQGAEWN
                     AVWSSVTSDDLVERTPESGPVFVPETAFKEQLAAFADLEPGTIGGPAQIASDDFFVGV
                     KREKVEESTVAYNEVSGDVKALLTEEKRREAQSSFFKELRGKASVIIHDFDLFPKPEA
                     PKDAAGVPVSADKAPASGPVSADQAPASGDAEKK"
     gene            2767..2861
                     /locus_tag="P5201_03295"
     tRNA            2767..2861
                     /locus_tag="P5201_03295"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2802..2804,aa:Ser,seq:gct)
     gene            2965..3041
                     /locus_tag="P5201_03300"
     tRNA            2965..3041
                     /locus_tag="P5201_03300"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2999..3001,aa:Arg,seq:acg)
     gene            3213..>4509
                     /locus_tag="P5201_03305"
     CDS             3213..>4509
                     /locus_tag="P5201_03305"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRV97588.1"
                     /translation="MLYRFSHSLPPFAANGKRSMYRVVPALVFFLLSLALVFPALKAN
                     AAASVRKDAFIAQLFQARGFSLPANEKSPVNAALEYNLTPLPEGKLSDPLTRREAIVY
                     AVHSLGLFFESRILADYPLPYKDVTGLLPEERGALAVALNMKPPLLKKGSATFSPSHA
                     VSPAEANEIAARVKAATRELRLSVDLSPLKGMTIRIRREGVLSILPKWRAVVNGFESR
                     EDADFFRGLLTERGVEATVDSYNYDWRVRSPIFDMYGGVRKFLSAASALGREGVVFAS
                     IPNWEMTDTPRFWTMMVFDPGAFELRPVFPSEGMAALAPLSSMLRDGAVAVVNGGYFG
                     TSGKGRGSPIGALMTDGVLLNPPFAGRTCFGWNSENRAVFGQLTWNGKVHLPGGFMEL
                     SGINRTLKGDGVVLFSPHFGMTTPLVETRAAEAVLGGSRV"
BASE COUNT          854 a         1308 c         1416 g          931 t
ORIGIN      
        1 tccccggagc tgaagcggcg ccccttctcg gccgccttgg cgaactctcg aaggggtctg
       61 tgctcttccc ggacttcaaa gaagccgccg cctgtttcgg ctcgtcggcg ctctctccgg
      121 agcgagtgcg ggatttcaag acggcgcact acctgctgca ccccgacaag aacgcgcacc
      181 tcccggaggc ggtcatcccc ggctatgaat cggcggcgct tgcggagagg ggagcggcga
      241 tccttgcgga atacggcaga atggaaaggg agctggcgaa gtatcccggt ttgtccgacc
      301 tgatgcgcac cgtggatatt ccgctgatcc ccgtgcttgt ggatatggag cagtacggca
      361 tctcctgctg ttcgcagacg ttcggcgcgc tggaaaagga tctttcggag cgcctctccg
      421 gcatcgagga ggagattgcg tcgacggcgg gggagcggat caacctgaat tccccgaaac
      481 aggtggcgtg gctgctcttc gagaagctcg gccttcccgc cgggaaaaag accaagacgg
      541 ggtactcgac ggatatctcc gtcctggagg ggctttccgc actcggtcgg cccttcgacg
      601 aggtgccgaa cctcctcata gagtacaggg agctctcgaa gatgctctcc ggttttgttc
      661 agccgctgac gaaagccggc gaggcggggg agggcgtgat tcacggcatc ttcgagcctg
      721 cggtgaccgg caccggaagg ttgagcagca gggaaccgaa cctccagaat ctccccacgt
      781 tcggcgagtg gtcgaagaga gtgaaggagg ggcttcagcc tcacggagag gatcgaatgt
      841 tcgtcgccgc ggactattcg cagatcgaac tccgcatcct cgcgcacctc tgcggcgata
      901 aacggttgct ggacgccttc gcctcgggac gggacattca taccgagacg gcttcgtggg
      961 tcttctccac cgagcccgcg tttgtaacgc cggaactgcg ccgcgtcgcc aagatgatca
     1021 acttcggtct gctctacgga atgagcgcgt tcggccttgc gcagcgcctc gccatagggc
     1081 ggcaggaggc ttcggacatc gtcaaacgct acttcgaggc gcttcccgga gtcaaggagt
     1141 acctcgacgc aggatatatc gaggcgaagg agcgagggta cacccgtacc gtcgccgggc
     1201 gtatccgtcc tctggccgag gtctccgtca gtcctcgcga ccgggacgcc ttgcggagag
     1261 tcgcggtgaa caccccgatc caggggagcg ccgccgatat tgcgcgcaaa gcgatgatcg
     1321 acttcgcgcg ccgctttccg aaggagggga gcgtacggct ggcgctccag gtgcacgact
     1381 cgctcgtctg cgaatgcgcg cgtgacgaag cgcctgcggt agagtcggag cttgtaaaaa
     1441 tcatggaggg cgcggccact ctctccgttc ccctcaaagt cgaggcgaaa aaaggaggaa
     1501 atctcgccga ggtgtaagcg aaaacgttct tctgtggtac aattctccct tggagtttgg
     1561 atattcgaaa gagaggaaga tgtccgttgt tgatgagaac cctgagatcc caggtgcggt
     1621 ggatcatgat tgcaatcgtg gtattgttcg tgctctctat ttttggtatg tacggttttg
     1681 acggagcccg gagagggcgc ccccagagcg gtgaaagcgg agattacgtt gtggctgaga
     1741 tcgacggcaa gccggtgatg cgttccgcgc tcgatcagag cgtcaggaac tacgtggaga
     1801 ggatgaacgt ccgggagatc aagccggagg atattccgca cctctaccag tcgacgctgg
     1861 aaagcctcgt gatcaatcat cgcctggcgc tggaggcgga gcagtccggc ctgaaggcga
     1921 cggatgaaga gcttgacgcc gctgtcaggg aagtaagcga gcagttcccg accaaggagg
     1981 ctttcatgca gtacctcgac cagtcagggg taaaaatggc cgctttccgc gagaaccttg
     2041 ccacgcagat tgtccagcag aagatggtgg agcgcgcttc cgccggtatc gccgtgacgg
     2101 aggaggaggc gctcgacttt tatgaaaaga cgaaagtgct cttcttccac tctcccgcag
     2161 ggtacacgat ggactacgcg aggctgaaga acgaagccgc cgccgcgaag atcgtcgaag
     2221 ccgtccgcca gggggccgag tggaacgccg tgtggagttc ggtgacatcg gacgatctgg
     2281 tggagcgcac gccggagtca ggtcctgtgt ttgttcccga aaccgctttt aaagaacagc
     2341 tcgccgcctt cgccgatctc gaaccgggta cgatcggcgg tccggcgcaa atcgccagcg
     2401 acgacttttt tgtcggggtc aagcgtgaaa aggtcgagga gtcgacggtc gcctacaacg
     2461 aggtgagcgg cgatgtgaag gcgcttctta cagaggagaa gcgcagggaa gcgcagtcct
     2521 ctttcttcaa ggagcttcgc gggaaggcgt ccgtgattat ccacgacttc gatctcttcc
     2581 ccaagccgga ggctcccaag gatgccgcgg gcgttcctgt atccgcagat aaagcgcctg
     2641 cgagcgggcc tgtttccgcc gaccaggcgc ctgcgagcgg agatgcggag aagaaataat
     2701 cgtcttttcg tgctttgcct attgacataa attccggcct tttatagaat agagcgcgct
     2761 gtttgtggtg ggttggccga gtaggtcgaa ggcgctcgcc tgctaagcga gtaggggggc
     2821 ttaaacctcc ctcgagggtt cgaatccctc acccaccgcc acttacacgc agcgtttctt
     2881 ttctccgggg atattgccaa gcgcaaaaga aagtgctaaa atcctccggg tgttcagagg
     2941 agcagggttc ctttgaacaa gtacgcgccg gtagctcaga tggatagagc ggcggtctac
     3001 ggaaccgcag gtcacgagtt cgaatcttgt ccggcgcgcc attttgttct ccagaaccgg
     3061 ggtccgatta tcgggtcccg gtttttttac gcctttttcc gtatgccgcg cttcgagcac
     3121 atctggtaca ctcgtcatgt tatatgcgcg cagttgcggc atctggaatt atcttcgccg
     3181 tttcgaatac agcgcctcaa ggagaataca ctatgctcta tcggttttct cattctcttc
     3241 cccccttcgc cgctaacggg aagaggagta tgtacagagt agtcccggcc ctcgtctttt
     3301 tcctcctctc tcttgccctt gtcttcccgg ccctgaaagc gaatgccgcc gcttccgtcc
     3361 gcaaggatgc gtttatcgcc cagctctttc aggcgcgggg gttttcgctg cctgcaaacg
     3421 agaagtcccc tgtcaacgct gctcttgaat ataacctgac gcctctcccg gagggaaagc
     3481 tttccgaccc cctcacgcgc agggaggcga tcgtgtatgc cgtccacagc ctcgggctct
     3541 tcttcgagtc gcgtatcctc gccgactatc cgcttcccta caaggacgtc accggtcttt
     3601 tgccggagga gcggggcgct ctggcggttg ccctgaacat gaagcctccc ttgctgaaaa
     3661 aaggctcggc gacattttcc ccgtcgcacg ccgtctctcc cgccgaagcc aatgagatcg
     3721 ccgcccgtgt gaaggcggcg acccgcgagc tccgcctctc ggtggacctc tctcctctca
     3781 agggaatgac gatccggata cgccgggaag gcgtgctctc aatccttccg aagtggaggg
     3841 cggttgtgaa cgggttcgag tcccgagagg acgcggactt tttccgcggg ttgcttacgg
     3901 aacgcggcgt ggaggctact gttgacagct acaactacga ctggcgcgtg cgctctccca
     3961 tcttcgacat gtacggcgga gtacggaagt tcctttccgc agcatccgcg ctcggcagag
     4021 agggcgttgt ttttgcgagc attcccaact gggaaatgac cgatacgccg cgcttctgga
     4081 cgatgatggt cttcgatccc ggggcgtttg aactgcgccc tgtctttccc tccgagggaa
     4141 tggcggcgct cgctcccttg agctccatgc tccgcgacgg cgccgtcgcc gttgtcaacg
     4201 gagggtattt cgggacgtcg gggaaaggcc gcggaagccc tatcggcgcg ctgatgaccg
     4261 acggggtgct gctgaatcct ccgttcgccg ggcgtacctg cttcggatgg aacagcgaga
     4321 acagggcggt cttcggtcag cttacgtgga acgggaaggt gcatcttccg ggagggttca
     4381 tggagctcag cggaatcaac cggacgctga agggggacgg cgttgtcctc ttctctccgc
     4441 acttcggcat gaccacccct cttgtcgaaa cccgcgcggc cgaggctgtt ctcggaggct
     4501 cgcgcgtgg
//
LOCUS       DAOTIL010000105         4456 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminobacteriaceae bacterium isolate AMR_MDS_3026
            NODE_23206_length_4456_cov_3.318564, whole genome shotgun sequence.
ACCESSION   DAOTIL010000105 DAOTIL010000000
VERSION     DAOTIL010000105.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868050
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminobacteriaceae bacterium (wastewater metagenome)
  ORGANISM  Aminobacteriaceae bacterium
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae.
REFERENCE   1  (bases 1 to 4456)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4456)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:19:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,224
            CDSs (total)                      :: 3,198
            Genes (coding)                    :: 3,184
            CDSs (with protein)               :: 3,184
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4456
                     /organism="Aminobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23206_length_4456_cov_3.318564"
                     /isolate="AMR_MDS_3026"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:3034609"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..473)
                     /locus_tag="P5201_03310"
     CDS             complement(<1..473)
                     /locus_tag="P5201_03310"
                     /inference="COORDINATES: protein motif:HMM:NF012382.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HRV97589.1"
                     /translation="MQPVKIAQRFQNIQPSGMLKIFQAAAAIDDLSNLGIGEPDFHTE
                     PKIVDAAAKAAKEGMTHYPPLTGYPELRKEICAYWKRHHDLEVTPEEIHITVGGIQAP
                     HLCFQAMLDPGDEVLIAEPCFTAYFQQIEFNGGVVVPVPSRAENGFFPTAEDFEKA"
     gene            complement(461..1693)
                     /locus_tag="P5201_03315"
     CDS             complement(461..1693)
                     /locus_tag="P5201_03315"
                     /inference="COORDINATES: protein motif:HMM:NF012885.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate carboxyltransferase"
                     /protein_id="HRV97590.1"
                     /translation="MGYREDGKWWVSPANYEAEVTKTFSFAPKIEILDTTLRDGEQQP
                     GIIFTKEDKVAIAKKLDEVGVHRIEAGTPAGSQEDADAIKAICDLGLKSKIFAFCRAM
                     PKDMELAKSLGVDGVLCEMIGSEQMLRYGKRWTPEQAIAACIEATQAAHELGMFVTFF
                     PADGSRAEMNYLLDFVQAVAEGGHIDSLALVDTFGTFSPEGAVYMLKKLKERFSFPIE
                     AHFHSDYDIGVATTLAALKEGASVAHVTVNGLGERAGSCPLEPLALSLEALYGQSTGI
                     VLNKLTELSKLVEELSRFPVPPIKPVVGNKLFGWETGLPSSLWTNAKTENPLIMLPYH
                     YSLTGREEPVLYIGKKSGKDNVKYWLAKTGLSLDDEGEKTLLQKVKDLSISLKRDLNE
                     DEFRGLVSRVKEESACNQ"
     gene            complement(1770..>4456)
                     /locus_tag="P5201_03320"
     CDS             complement(1770..>4456)
                     /locus_tag="P5201_03320"
                     /inference="COORDINATES: protein motif:HMM:NF024938.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="acetate--CoA ligase family protein"
                     /protein_id="HRV97591.1"
                     /translation="FADSGSASALEKAKSFGVPAVSLDIREFYARMNAPLKDREVRAA
                     YDREILAMLEPFSPDMLLLAGYVWATTDVITGSIPTVGVHPADLAVRKNGERAYAGAD
                     GVGATLAGGEHEIRATSYLATPVLDGGPMLIVSPPSPIDSDEGMDPKDRFRKYLSLVN
                     EQARETGARTVYEIARGHFAVNADGIAVHKGRSLPDGLRFESRPPFVPDISLFSPRSI
                     AVVGASASGTSLGSAVLRNILSYGFKGEVYPVNRKGEDAEGLKGYASVLEIPGDVDLA
                     MITVPGAAAVEVAEECGRKGVRNLVTLSAGFRETGEEGARAENALLDIVSKYSMRLLG
                     PNCMGLLNTSPDVRLHANMLQTVPKRGGIGLITQSGAIGAALLDFAPSLGLGFSLIAS
                     TGNQPDMTINDLLPLYAEDDNTTVILAYLETLPDAGRFARILTEAAAKKPVIVVKSGM
                     TEAGAAAAKSHTGSLAGKSRIAEALLDKTGAIRAATLEEAFLLASALSTQPRVKGNRV
                     GVISNAGGPGTLVADELSRRGFSLPFLPEATREALAAQVLPQASTANPLDLVATAKPE
                     HYAAAAKAMVESNLYDAFVVVMVPPTGVETGPVAEAMIPVLKASGLPVTSCFFGPSMG
                     EGGRAAMLNAGIPSFPFPEQTASVLSLMREKTLSPCAEKPNTQTPSLLRKGALRKRLA
                     ASADGFLLPALCEELLEAYGIPLPLSAMVTAPEEVKGASFLYPVVAKIDHPDVLHKSD
                     AGGVVLNIADEKLLRGVVETLLARFPGARGVLVQEQIGKGIELILGADSDPLLGHAVL
                     VGWGGTGVEVFSDVALGHVPFSRAEGMRLLERLQCFPLLKGYRGKPGVDLEGLLDLLQ
                     KLQRLLLDFPEIEEMDLNPLIWDSKRFIAADYRIKTRS"
BASE COUNT          762 a         1429 c         1388 g          877 t
ORIGIN      
        1 gccttctcga agtcctcggc ggtcgggaaa aatccgttct cggcccgcga aggaacgggg
       61 accacaacgc cgccgttgaa ctcgatctgc tggaagtagg ccgtgaagca cggctcggca
      121 ataagcacct cgtcgcccgg gtcgagcatc gcctggaagc agaggtgcgg cgcctggatg
      181 ccgcccacgg tgatgtgaat ctcctcgggc gtgacctcca ggtcgtgatg ccgcttccag
      241 taggcgcata tctccttgcg cagctccgga taccctgtga ggggcgggta gtgcgtcatc
      301 ccctctttcg cggccttcgc cgccgcgtcg actatcttcg gctcggtgtg aaagtccggt
      361 tcgccgatcc cgaggttgct caggtcgtcg atggctgcgg ccgcctggaa gatcttcagc
      421 atccccgacg gctggatgtt ctgaaagcgt tgcgctattt tcactggttg catgcactct
      481 cctccttcac ccgggagacc agcccccgga actcgtcttc gttgaggtcg cgcttcagag
      541 agatggagag atccttcacc ttctggagca gcgtcttctc tccctcatca tccaacgaaa
      601 gtccggtttt tgcaagccag tattttacgt tgtccttgcc gctctttttg ccgatgtaga
      661 gcaccggctc ctcccgtcca gtcagcgagt agtggtaggg gagcatgatg agcgggtttt
      721 ccgtcttcgc gtttgtccag aggctcgacg gaagtccggt ttcccagccg aagagcttgt
      781 tgccgacgac cggtttgatg ggcggcacgg ggaagcggga aagctcctcc accagtttcg
      841 agagttccgt gagcttgttg aggacgattc cggtggactg gccgtagagc gcctcaaggg
      901 agagcgccag cggctcgagc gggcagcttc ccgcgcgctc gccgagaccg ttcaccgtca
      961 cgtgggcgac cgacgcgccc tctttgagcg cggcgagcgt cgtcgccacg ccgatgtcgt
     1021 agtcgctgtg gaaatgcgcc tcgatcggga aggagaaacg ctctttgagt ttcttgagca
     1081 tgtagaccgc tccttcggga gagaatgtgc cgaaggtgtc aacgagcgcc agcgagtcga
     1141 tgtgcccgcc ctcggcgacc gcctgcacga agtcgagcag gtagttcatc tcggcgcgcg
     1201 agccgtctgc ggggaagaat gtgacgaaca tccccagctc gtgggctgcc tgcgtcgcct
     1261 cgatgcacgc ggcgatggcc tgctcgggcg tccagcgctt gccgtagcgc agcatctgct
     1321 cgctgccgat catctcgcag agcacgccgt ccacgccgag cgacttcgcc aactccatgt
     1381 ccttcggcat cgcacggcag aaggcgaaga tcttcgactt caatccaagg tcgcagatgg
     1441 ccttgatcgc gtcggcgtcc tcctgggatc ccgcgggcgt tcccgcctcg atcctgtgga
     1501 cgcccacctc gtcaagcttc ttcgcgatgg cgaccttgtc ctccttcgtg aagatgatcc
     1561 ccggctgctg ctcgccgtcg cggagcgtcg tgtcaagtat ttcgatcttc ggtgcgaaag
     1621 agaacgtctt cgtcacttcc gcctcgtaat tcgcgggtga aacccaccac ttgccgtctt
     1681 ctctgtatcc cattctcaat tcctcctcga aagtagtgtt cttctttgcg tccggcggcg
     1741 tgggtgtccc cgccgtccgg ttcttcgtgt caagagcgtg tttttattct gtagtccgcc
     1801 gcaataaaac gtttgctgtc ccagataagc ggattgaggt ccatctcctc gatctcggga
     1861 aaatccaaca gcagtcgctg aagcttttgc aacaggtcga gcagtccttc caggtcgacg
     1921 ccgggcttgc cccggtaccc cttcagcagc ggaaaacact gaaggcgttc gagaaggcgc
     1981 atcccctcgg cccgcgaaaa gggaacgtgc cccagcgcaa cgtcggagaa gacctcgaca
     2041 cctgtcccgc cccacccgac gagcacggcg tgcccaagga gcgggtcgct gtccgcgccg
     2101 aggatgagtt caatcccttt tcctatctgt tcctgcacca gtaccccacg agctcccgga
     2161 aaacgggcga ggagtgtttc aacgacgccg cggagcaact tctcgtccgc gatgttgagc
     2221 actacgccgc ccgcgtccga cttgtggagc acgtcggggt ggtctatctt cgccacgacg
     2281 gggtagagga acgaagcgcc cttgacctct tccggcgcgg tgaccatggc ggagaggggg
     2341 agaggaatgc cgtatgcttc caaaagctcc tcgcagagcg caggcagaag gaaaccgtcc
     2401 gcggaagcgg cgagtcgttt ccgtagagct cccttgcgga gcagcgaggg cgtctgcgta
     2461 ttcggttttt ccgcacacgg ggaaagagtc ttctcgcgca tcaaggagag tacggaagca
     2521 gtctgttccg ggaacgggaa cgacgggatt cccgcgttca gcatcgccgc ccgcccgccc
     2581 tctcccatcg aagggccgaa gaaacagctc gtcacaggca gtccggacgc cttgaggaca
     2641 ggtatcatcg cctcggccac ggggcccgtc tcgacgcctg tcggcggtac catcaccacc
     2701 acaaatgcgt cgtacaggtt gctttcgacc atcgccttcg cggcggccgc gtagtgctca
     2761 ggcttcgccg tagccaccag gtcgagcgga ttcgccgtcg acgcttgagg aagtacctgc
     2821 gcggcaagcg cctcgcgcgt cgcttcggga aggaagggaa gcgagaagcc tcttctgctc
     2881 agttcgtccg cgactagcgt tcccgggcct ccggcgttgc tgatcacgcc gacccggttc
     2941 cctttcactc gaggttgcgt cgagagcgcg gaggcgagca ggaaggcctc ttcgagcgtt
     3001 gctgcgcgga tggccccggt cttgtccaga agcgcctccg cgatacgcga cttcccggcg
     3061 agactccccg tgtgcgactt ggccgccgcc gctcccgctt ccgtcatgcc cgacttgacc
     3121 acgatcaccg gcttcttcgc ggccgcttcg gtcaggatgc gcgcgaatcg ccccgcgtcg
     3181 gggagcgtct ccaggtacgc gaggatcacg gtggtattgt cgtcctccgc atagagtgga
     3241 agaagatcgt tgatcgtcat gtccggctgg tttcccgtgg aggcgatcag tgagaagccc
     3301 agcccgagcg aaggggcgaa gtcgagcagc gcggcgccga tcgccccgct ctgcgtgatc
     3361 aggccgatgc cgcccctctt cggaacggtt tggagcatgt tcgcgtgcag ccggacgtcg
     3421 ggggatgtgt tcagcagccc catgcagttc ggcccgagca ggcgcatcga atacttcgat
     3481 acgatgtcaa gaagcgcgtt ttccgctcgc gctccctctt cgcccgtctc gcggaagccg
     3541 gcggagagcg tgacgagatt ccgcaccccc ttgcggccgc actcttcggc aacctcgacg
     3601 gctgccgctc ccggaacggt gatcatcgca aggtcgacgt ctccgggaat ctccagaacg
     3661 gaggcgtacc ccttcaggcc ttcggcatcc tcccccttcc ggttcacagg gtacacttct
     3721 cccttgaatc cgtaggagag gatgttccgc agtacggcgc tcccgaggct cgtccccgac
     3781 gcagaagcgc cgaccacggc gatggagcgc ggagaaaaga gggagatgtc cgggacgaag
     3841 gggggccggc tctcgaaccg taatccgtcg gggagcgatc ttcccttgtg cacggcgatt
     3901 ccgtcggcgt tcacggcgaa gtgtccgcgg gcgatctcgt acaccgtcct ggctccagtt
     3961 tcgcgggcct gttcgtttac aagagagagg tacttccgga agcggtcctt cgggtccatt
     4021 ccctcgtccg aatcgatcgg cgaaggaggg gagacgatca gcatcggtcc gccgtccagt
     4081 accggcgtgg cgaggtagga ggtcgccctg atctcgtgtt ctccccccgc gagggtggcg
     4141 ccgactccgt cggcccctgc gtacgcccgt tcgccgttct tgcggacggc gaggtcggcc
     4201 ggatggacgc cgacggtggg aatcgagccg gtgatgacgt ccgtcgtcgc ccagacgtac
     4261 cctgcgagca gcagcatatc gggggagaac ggctccagca tggccagaat ctcccggtcg
     4321 tacgcagcac gaacctccct gtccttcagc ggcgcattca tccgggcgta gaactcccgg
     4381 atgtccaaag agacggcagg aaccccgaac gatttcgcct tctcaagtgc agacgcgctg
     4441 ccggaatcgg cgaaga
//
LOCUS       DAOTIL010000106         4454 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminobacteriaceae bacterium isolate AMR_MDS_3026
            NODE_23220_length_4454_cov_3.389634, whole genome shotgun sequence.
ACCESSION   DAOTIL010000106 DAOTIL010000000
VERSION     DAOTIL010000106.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868050
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminobacteriaceae bacterium (wastewater metagenome)
  ORGANISM  Aminobacteriaceae bacterium
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae.
REFERENCE   1  (bases 1 to 4454)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4454)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:19:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,224
            CDSs (total)                      :: 3,198
            Genes (coding)                    :: 3,184
            CDSs (with protein)               :: 3,184
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4454
                     /organism="Aminobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23220_length_4454_cov_3.389634"
                     /isolate="AMR_MDS_3026"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:3034609"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..820
                     /gene="hutI"
                     /locus_tag="P5201_03325"
     CDS             <1..820
                     /gene="hutI"
                     /locus_tag="P5201_03325"
                     /EC_number="3.5.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008521127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050480 - imidazolonepropionase activity
                     [Evidence IEA];
                     GO_process: GO:0006548 - histidine catabolic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="imidazolonepropionase"
                     /protein_id="HRV97592.1"
                     /translation="GTTTVEMKSGYGLDTESELKMLSAIRRVGRETPLRVVPTFMGAH
                     AIPEVFKGNGDGYVDLMVGEMLPAVAEQGIARFCDVFCEEGVFSPEQTRKVLVEARKY
                     GLGLKIHADEVHDTGGAALAAELGAVSAEHLLAASDEGIEALAASGTVAVLLPATAYS
                     LRKPFARARTMIEKGVPVALATDCNPGSSFTESMPFVFGLAVLVMGLTVEEALVATTK
                     NSAHAIGLGAECGTLEPGKNADFLLLDGETPAVLAYHAGVSPVKSVFKKGERVA"
     gene            820..2361
                     /gene="hutH"
                     /locus_tag="P5201_03330"
     CDS             820..2361
                     /gene="hutH"
                     /locus_tag="P5201_03330"
                     /EC_number="4.3.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012869663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004397 - histidine ammonia-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0006548 - histidine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine ammonia-lyase"
                     /protein_id="HRV97593.1"
                     /translation="MIGERKNRVRLDGMSLTLEDVLRVARDGWGVELDEEAARKVEEA
                     SAAVKAWENSDDVVYGITTGFGDLATVNISRKDRRLLQENLLKSHACGVGEPFSEEIT
                     RAMMLLRINSLIRGFSGISLTTLHQFVAFLNLGITPVVPSQGSVGASGDLCPLSHLAL
                     PLLGLGKVRYKGRVMLAAKALQTAGLQPVQLGAKEGLALNNGTASMGAMGVVALMAAE
                     DLAKTADIAAALSIEALHGVPYAFDARTHALRAHRGQGRVAENIRRLIEGSEIIEKHR
                     FGRVQDAYSLRCVPQVHGATRDALEYVRRTLETEINSVTDNPIIFTEDGVAISGGNFH
                     GQPLALAMDFFGIAVAELASISERRQARLVDTSLSGLPPFLVEDSGLNSGFMIAQYTS
                     AALVSENKVLAHPSSVDSIPTSANQEDHVSMGAFSARKALMILDNARKVLAIELFAAS
                     QGLDFSRLLKPGAGTVAAHDCVRSVVPYLKHDEYLHPLIERVEALLSRGAVVRAVEEA
                     IGPLN"
     gene            2491..>4454
                     /locus_tag="P5201_03335"
     CDS             2491..>4454
                     /locus_tag="P5201_03335"
                     /inference="COORDINATES: protein motif:HMM:NF013867.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CheR family methyltransferase"
                     /protein_id="HRV97594.1"
                     /translation="MTDIMRNDDLFPQKNDMPFVNASRDIPRIICLGASAGGLEALER
                     FFRHLPEDVQDIFVIVQHLSPDHKSIMAELLHRYTSLPVVVVDDGMPPQPGRIHLIPP
                     GKEMVVENGAFRLRDRVLREPHLPINVFLQSMAREYGASCVAVILSGTGSDGAQGIKA
                     VHAAGGAVFAQDVESSRFDGMPRNAALTCVVDFVGSPEQIVSALFKTAVDETGEPEIY
                     EERERLCEKDALEPKQLIFRKLSDAFGIDFSQYKDGTILRRLDRRMMMRAVADMSEYA
                     ELLEGDSEEVDHLYRDLLIDVTSFFRNPEAFSYLAETALPDLLARHPENEEFRVWSVA
                     CASGEEAYSLAMILDEVMANRSQKRSVRIFATDIHKSSLKTASAGYYSNEKLANVSTA
                     RREKYFYRTGADGWTVRPNIRRMITFAPHNILSDPPFLHMHLISCRNMLIYLEKSAQI
                     ETLTRFSAALEQQGILFLGSSESVLDTQSEFLTVDSRLKIYRKQGTITSRPLYSLLPE
                     KKKATAGSERGSSKMPLSSLYPLLLERCMPTGFLVNSKGDLLHTFGGAGKYLQIIGPV
                     RTDIGSILHGNLRVAVMTAIERSRKNRTPVRFSDVSHPLPGAPLNTVEVSVEPLEERT
                     DSRPEEGNLFFFIRLEEMKPSLAAKAESFSV"
BASE COUNT          844 a         1377 c         1381 g          852 t
ORIGIN      
        1 cgggacgacc accgtcgaga tgaagagcgg gtatggactc gacacggaga gcgagttgaa
       61 aatgctctcg gcgattcgcc gcgtcgggcg cgaaacgccc ttgcgcgtcg ttcccacctt
      121 tatgggggcg cacgcgattc cggaggtctt caaggggaac ggcgacggtt acgtcgatct
      181 tatggtcgga gagatgcttc ccgccgtcgc ggaacaggga atcgcccgct tctgcgacgt
      241 cttctgcgag gagggagtct tctccccgga gcagacgcgg aaagtgctcg tcgaggcccg
      301 aaaatacggg ctcggcctga agattcacgc cgacgaggtg cacgacaccg gcggggccgc
      361 gcttgccgcc gaactcggcg ccgtttccgc ggagcatctt ctcgccgcga gcgacgaagg
      421 gatagaagcc ctcgccgcga gcggaacggt cgccgtactg ctccccgcca cggcgtacag
      481 cttgcgcaag ccgttcgcac gcgcccggac catgatcgag aagggggttc ccgtggccct
      541 tgcgaccgac tgcaaccctg gttcctcctt cacggagtcc atgcccttcg tcttcggcct
      601 ggccgtactc gtgatgggac tcactgtgga agaggcgctt gtggcgacga cgaagaacag
      661 cgcgcacgcg atcggcctcg gggccgagtg cgggacgctc gagccgggaa agaacgccga
      721 cttcctcctc ctcgacggag agactccggc cgtcctggcc taccacgccg gggtctcgcc
      781 ggtgaagtcc gtcttcaaga aaggggagcg tgtcgcatga tgatcggtga gcggaagaac
      841 agagtacgtc tcgacgggat gtcgcttacc ctcgaggatg tgctccgcgt cgcgcgcgac
      901 gggtggggag tcgaactgga cgaagaggcc gcccggaagg tggaggaggc gtccgccgcc
      961 gttaaagcct gggagaactc cgacgacgtc gtctacggca tcaccacggg cttcggcgac
     1021 ctcgccacgg tgaacatctc ccgcaaggat cgtcgcctgc tgcaggagaa tctcttgaag
     1081 agccacgcct gcggcgtcgg cgagccgttc tccgaggaga ttacgcgggc catgatgctg
     1141 ctgcggatca acagtctcat cagaggattc tcgggtatca gtctcacaac gctgcatcag
     1201 ttcgtcgcat ttctgaacct gggaatcacc ccggtcgtcc cgtcgcaggg gtcggtgggc
     1261 gccagcggcg atctctgccc tctctcgcac ctcgcgctcc cgctgctggg gctcggaaaa
     1321 gtgcgttata aggggcgcgt catgctcgct gccaaagcgc tccagacggc ggggctccag
     1381 cctgtgcagc tcggggcgaa ggagggactc gccctgaaca acggcacggc ctcgatgggc
     1441 gccatgggcg tagtggcgct gatggccgcc gaggaccttg ccaagaccgc ggacatcgcg
     1501 gcggcgctct ccattgaagc gcttcacggc gtcccctacg ccttcgacgc gcggacgcac
     1561 gcgctccgcg cccacagagg gcaagggcgc gtagccgaga atatccgccg actgatcgag
     1621 gggagcgaaa ttatagagaa gcaccgcttc ggcagggtgc aggacgccta ctccctccgc
     1681 tgcgtgccgc aggttcacgg cgccacccgc gacgcgctgg agtacgtgcg gaggacgctg
     1741 gagacggaga tcaactcggt caccgacaat ccgatcatct tcaccgagga cggcgtggcc
     1801 atcagcggcg gcaacttcca cggccagccg ctcgcccttg cgatggactt cttcggcatc
     1861 gccgtggcgg agcttgcgag catctccgag cgcaggcagg ccagactggt ggacaccagc
     1921 ctctcagggc tgcccccctt cctggtggaa gacagcggcc tgaacagcgg tttcatgatc
     1981 gcgcagtaca catcggcggc ccttgtctcg gagaacaaag tgcttgcgca cccctcctcc
     2041 gtcgactcca tccctacctc ggccaatcag gaggaccacg tgtcgatggg cgcgttctcg
     2101 gcgcgaaagg cgctcatgat cctggacaac gcgcggaagg tgctcgccat agaactcttc
     2161 gccgcctccc aggggctcga ctttagccgt ctgttgaaac cgggcgcggg aacggtcgcc
     2221 gcgcacgact gcgtcaggag cgtcgtcccc tacctgaagc acgacgagta cctccatccg
     2281 ctcatcgagc gcgtggaggc gcttctgagc cgcggcgcgg tggtccgggc tgtggaagaa
     2341 gccatcggac cgctgaactg acaaaagacg gaatctcgag aggctcgcct tcggcgggcc
     2401 tctcgccgta tgatcagctc tccttcctcc tgcgaagacg ggtatgctat actctttttc
     2461 agtacatacc gcgaaacagg ggggatttca atgactgata tcatgcgaaa tgatgatctc
     2521 ttcccgcaga aaaacgacat gcctttcgtg aatgcatccc gggatatccc ccgcatcatc
     2581 tgtctcggcg cctcggccgg aggcctggag gctcttgagc gattcttccg ccatttgccg
     2641 gaggacgttc aggatatctt cgtcatcgtc cagcatcttt ctccggacca caagagcatc
     2701 atggcggaac tgctccaccg gtacacgtct cttccggtag ttgttgttga cgacggaatg
     2761 ccgccgcaac cgggacgtat acacctgatt ccgccgggca aagagatggt ggtcgagaac
     2821 ggggcgtttc gtttgcggga cagagtcctt cgcgaaccgc atctccccat caatgttttt
     2881 ttgcagagca tggccagaga gtacggagct tcttgcgtcg ccgtcatcct ttccggtacg
     2941 gggagcgacg gcgcgcaggg aatcaaggca gtccacgcgg cgggtggcgc ggtgtttgcc
     3001 caggacgtcg aaagctcccg tttcgacgga atgccgcgca acgcggccct gacctgcgtc
     3061 gttgattttg tcggttcccc ggagcagatc gtctccgctc tcttcaaaac cgccgtcgac
     3121 gaaaccggcg agccggagat ctacgaagag cgcgagcgtc tttgcgagaa ggacgctctt
     3181 gaaccgaagc agctgatctt ccgaaagttg agcgacgctt tcggtatcga cttctcccag
     3241 tacaaggacg gcacaattct ccgccgtctg gaccggcgga tgatgatgcg cgccgtcgct
     3301 gatatgtccg agtatgcgga gttgctcgaa ggagattctg aagaagtgga tcatctctac
     3361 agggatctgc ttatcgacgt cacgtcgttc ttcagaaatc cggaggcctt ctcctatctt
     3421 gccgagacgg cgcttcccga tctgctggcg cgccacccgg aaaacgagga gtttcgcgtc
     3481 tggagcgtag cgtgcgccag cggcgaggaa gcctactccc tcgctatgat tctcgacgag
     3541 gtgatggcga accgctcgca gaaacggagc gtccgtattt tcgcgacgga tattcacaag
     3601 agttccctga agacggcgag cgcggggtat tattcgaacg aaaagctcgc caacgtttcg
     3661 acggcgcggc gtgaaaaata tttctacagg acgggcgccg acgggtggac ggtgcgcccg
     3721 aatatcaggc ggatgattac ctttgcgccg cacaatatcc tctccgatcc gccttttttg
     3781 cacatgcacc ttatttcctg ccgcaacatg ctcatctacc ttgagaaaag cgcccagatc
     3841 gaaacgctga cccgcttttc ggctgctttg gagcagcaag gcattctctt cctcggctcc
     3901 agcgagagcg tcctcgacac gcagagcgag tttttgacag tggacagccg tctgaagatc
     3961 taccggaagc aggggacgat tacttcccgg ccgctctact ccctccttcc ggaaaagaaa
     4021 aaggcgactg ccggttccga acggggaagt tcgaaaatgc cgctttcttc gctgtatccg
     4081 cttcttcttg agcgctgcat gccgacgggc tttctcgtga actcgaaggg cgatttgctc
     4141 cacactttcg gcggggcggg aaaatatctg cagatcatcg gccctgtgag gacggatatc
     4201 ggcagcattt tgcacgggaa cctgcgcgtc gccgtcatga cggcgatcga gcgttcccgg
     4261 aaaaacagga cccccgtccg cttttcggat gtttcccacc cgcttccagg cgctccgctg
     4321 aacactgtgg aggtctccgt ggagccgctc gaagagcgga cggacagccg acctgaggag
     4381 ggcaatctct tctttttcat tcgcctggag gagatgaagc cctcgcttgc cgcgaaggcg
     4441 gagtcattct ccgt
//
LOCUS       DAOTIL010000107         4451 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminobacteriaceae bacterium isolate AMR_MDS_3026
            NODE_23240_length_4451_cov_3.079845, whole genome shotgun sequence.
ACCESSION   DAOTIL010000107 DAOTIL010000000
VERSION     DAOTIL010000107.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868050
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminobacteriaceae bacterium (wastewater metagenome)
  ORGANISM  Aminobacteriaceae bacterium
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae.
REFERENCE   1  (bases 1 to 4451)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4451)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:19:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,224
            CDSs (total)                      :: 3,198
            Genes (coding)                    :: 3,184
            CDSs (with protein)               :: 3,184
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4451
                     /organism="Aminobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23240_length_4451_cov_3.079845"
                     /isolate="AMR_MDS_3026"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:3034609"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1353)
                     /locus_tag="P5201_03340"
     CDS             complement(<1..1353)
                     /locus_tag="P5201_03340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008712948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit B"
                     /protein_id="HRV97595.1"
                     /translation="MSATEYIGVRKITGPFVLLDGIQGAGYDEIVLVSTPDREARKGR
                     VVLVDEKATLVQVFTGTEGLVPETASVRFLGRELEMALAPSMLGRTFSGLGEPRDGYG
                     PVLGGVRRPVRGAPINPMAREYPRNFISTGISSLDVLLTLIRGQKLPIFSGNGLPHNA
                     LAVQIATQSKLLGAENFVVVFAGIGIKNDDAAYFQSHLASEGRGGNLVLFLNLADDPV
                     IERIATPRLALTTAEYLAFDLGMHVLVILTDMTSYCEALRELSVARGEVPSRKGYPAY
                     LYSDLASLYERAGALRNSKGSVTQIPILSMPNDDITHPIPDLTGFITEGQIVLSRDLE
                     ARGIYPPVDVLPSLSRLMKDGIGKGSTREDHANLAGQLFASYSRVQEVRALAGVVGTD
                     ELSRADKASLAFGDSFERLFVNQGMEENREIGHSLDLGWKLLSMLPVQELSRLSMEEI
                     E"
     gene            complement(1357..3135)
                     /locus_tag="P5201_03345"
     CDS             complement(1357..3135)
                     /locus_tag="P5201_03345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006583907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit A"
                     /protein_id="HRV97596.1"
                     /translation="MNSTGKIVAINGPVIRASGMRSFAMREMVQAGRLLLLGEIIRLD
                     GDDALVQVFEDTTGLRTGEPVTGTGGPLSMSLGPGLMGSIFDGIGRPLGKLLEREGPF
                     VTRGVSIPQLDPDAMWDVTPLGKVGDVVTPGAPLALVKETSLVEHRILTPCDIEGEIL
                     SILPPGLHKASSVVARVRDGHGRERSIPMIRKWPVRTPRPIAERLLPDEPLLTGQRII
                     DGLFPISKGGVAAIPGGFGTGKTVTQHQLAKWSDAQIVVYIGCGERGNEMTQVLEEFP
                     VLEDPRSGKPLMERTVLIANTSNMPVAAREASIYTGITIAEYYRDMGYDVALMADSTS
                     RWAEALREISGRLEEIPAEEGFPAYLPTRIAEFYERAGKVTTLGGTRGSISIIGAVSP
                     PGGDFTEPVTRHTKRFIRCFWALDQQLARSRHFPAISWIDSYSEYGDEVKSWYASHVD
                     PAWATLREKARTLLAEDDKIQQIIRLVGEDVLPDDQRLIALTASFLKNGYLQQGAFGP
                     DSYCPPKKGIAILNLLIAFHQKAEDLLSQGCPLSLLKGIDELVELTHLREIPAERLER
                     FEELKHDLFERMESINRERIVSRREV"
     gene            complement(3146..3862)
                     /locus_tag="P5201_03350"
     CDS             complement(3146..3862)
                     /locus_tag="P5201_03350"
                     /inference="COORDINATES: protein motif:HMM:NF014093.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0033178 - proton-transporting two-sector
                     ATPase complex, catalytic domain [Evidence IEA];
                     GO_function: GO:0046961 - proton-transporting ATPase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit E family protein"
                     /protein_id="HRV97597.1"
                     /translation="MSNSISGDGRGRELQNPAKRAAEEQNLLEMQRTPVRDDSNRKLL
                     ALKNLLLKKGDEEREVILDSARREATAWLKEQNEGLDRMVEQIHADAVKNAEEIAKRQ
                     IARAESLRAKDRLRLQNTLLDEAMAMLQRALTSLREREDYPLVLAGLVLEAAKDLPAG
                     TAALISLAVDDAPLGDALAERVGKKRPDMTFTFDPSPVPISGGAWLSAPDGSWRAAAD
                     LREVAAELKDSLAERLLAAL"
     gene            complement(3859..4173)
                     /locus_tag="P5201_03355"
     CDS             complement(3859..4173)
                     /locus_tag="P5201_03355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014807716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit F"
                     /protein_id="HRV97598.1"
                     /translation="MKGFLISDNHDSLVLLRLAGIPGVVVHGEEETTTAVIDVLRNRP
                     DTGVLVMTERAAAEVPLLVKSLREREDPPLLVEIPDRHGSLREPDFLTRYIRNAIGVK
                     IE"
     gene            complement(4187..>4451)
                     /locus_tag="P5201_03360"
     CDS             complement(4187..>4451)
                     /locus_tag="P5201_03360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012869272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ATP synthase subunit C"
                     /protein_id="HRV97599.1"
                     /translation="ALSAGSAAAAEGAAAVEGAGLGFLGASLATGMACLGAGLAVAIV
                     GAAALGVVGEKPSMLGTTLIYLGLAEGIAIYGVIVSLLILGKL"
BASE COUNT          717 a         1596 c         1376 g          762 t
ORIGIN      
        1 ctcgatctct tccatcgaga ggcgcgatag ctcctgcacc ggaagcatcg agagcagctt
       61 ccaccccagg tcgagcgaat ggccgatttc gcggttctcc tccattccct ggttcacgaa
      121 gaggcgctcg aaggagtcgc cgaaggccag cgacgccttg tcggccctgc tcagctcgtc
      181 ggttcccacc actccggcga gggcgcgcac ctcctgcacc ctgctgtacg aggcgaaaag
      241 ctgcccggcg aggttcgcat ggtcctcccg cgtggacccc ttcccgatac cgtccttcat
      301 caggcgcgaa aggctcggca ggacgtccac cggagggtag atccccctcg cctccagatc
      361 gcgcgaaagc acgatctgcc cctccgtgat gaagcccgtc aggtcgggga tggggtgggt
      421 gatgtcgtcg ttgggcatgc tgaggatggg gatctgcgtc acgctcccct tgctgttccg
      481 cagcgctccg gcgcgttcgt agagggaggc gaggtcgctg tacagatacg cgggataccc
      541 cttgcggctg ggcacttcgc cgcgggccac cgacagctcc ctgagcgctt cgcagtagct
      601 cgtcatgtcc gtcaggatca cgagcacgtg catccccaga tcgaaggcaa gatactcggc
      661 ggtcgtcagc gcgaggcgcg gcgtcgcgat gcgctcgatc acggggtcgt ccgccaggtt
      721 caggaagagc accagattgc ccccccgccc ctccgaggcg aggtgcgact gaaagtacgc
      781 cgcgtcgtcg tttttgatgc cgatcccggc gaagacgacc acgaaattct ccgcccccag
      841 cagcttcgac tgcgtcgcga tctgcaccgc gagcgcgttg tgcggcaggc cgttgcccga
      901 gaagatcggc agcttctgtc cgcggatcag cgtcagcagc acgtcgagcg acgagatccc
      961 cgtcgagatg aagttcctcg ggtactcgcg ggccatcggg ttgatcggcg cgccgcgcac
     1021 cggacgcctg accccgccca ggaccgggcc gtagccgtcg cggggctctc ccagtccgga
     1081 gaacgtccgc ccgagcatcg acggcgccag cgccatctca agctcgcgcc cgagaaagcg
     1141 tactgaggcc gtctcgggga cgagcccctc cgtcccggtg aagacctgca ccagcgtcgc
     1201 cttttcgtcg accagcacca cgcgcccctt gcgcgcctct ctgtcgggag tggagacgag
     1261 cacgatttcg tcgtaccccg cgccctggat gccatccagc agcacgaagg ggccggtgat
     1321 cttccgcacg ccgatatact ccgttgccga cacggtctac acctccctcc gggagacgat
     1381 gcgctcccgg ttgatgctct ccatgcgttc gaagagatcg tgtttcagct cttcgaagcg
     1441 ttcgagccgc tccgccggaa tctcccgcag atgcgtcagc tccaccagtt cgtcgatccc
     1501 cttcagcagc gagagcgggc acccctgcga aagcaggtcc tccgcctttt ggtggaaggc
     1561 gatcagcagg ttcaaaatcg cgatcccctt cttcggcggg cagtacgagt cggggccgaa
     1621 agccccctgc tgcaggtagc cgttcttcaa aaacgacgcc gtcagcgcga tcagccgctg
     1681 gtcgtccgga agcacgtcct cgcccaccag gcggatgatc tgctgaatct tgtcgtcctc
     1741 ggcaagaagc gtccgcgcct tctcgcggag cgtcgcccac gccgggtcca cgtgcgaggc
     1801 gtaccatgac ttcacctcgt cgccgtactc gctgtacgag tcgatccagc tgatggcggg
     1861 gaagtggcgc gaccgcgcga gctgctggtc cagcgcccag aagcagcgga tgaagcgctt
     1921 cgtgtgccgc gtcaccggct cggtgaagtc gccccccgga ggggagaccg cgccgatgat
     1981 cgagatgctc ccccgcgtgc cgcccagcgt ggtcaccttg ccggcgcgct cgtagaactc
     2041 cgcgatccgc gtgggaaggt aggccgggaa gccctcttcc gccggtatct cctcaagccg
     2101 tccggagatc tcgcggagcg cctccgccca gcgggaggtg gagtcggcca tcagcgccac
     2161 gtcgtacccc atgtcgcggt agtactccgc gatggtgatc cccgtgtaga tggacgcctc
     2221 gcgcgccgcc accggcatgt tcgacgtgtt cgcgatcagg acggtgcgct ccatcagcgg
     2281 cttccccgag cgaggatcct caagcaccgg aaactcctcg agcacctgcg tcatctcgtt
     2341 gccgcgctcg ccgcagccga tgtacaccac gatctgcgcg tcgctccact tggccagctg
     2401 gtgctgcgtc accgtcttcc ccgtgccgaa gccgcccgga atggcggcga ctccgccctt
     2461 cgatatgggg aagagcccgt cgatgatccg ctgtccggtc aagagcggct cgtcgggcag
     2521 caggcgctcc gctatcggac gcggcgtgcg caccggccac ttgcggatca tggggatgct
     2581 ccgctccctc ccgtgcccgt cgcgcacgcg cgcgaccacg gacgacgcct tgtggagtcc
     2641 gggggggagg atggagagaa tctctccttc gatgtcgcac ggcgtcagaa tacggtgctc
     2701 caccagcgac gtctctttga ccaaagcgag gggagcgccg ggcgtcacca cgtcgccgac
     2761 cttccccagc ggggtcacgt cccacatcgc gtcgggatcg agctgcggga tcgaaacgcc
     2821 gcgcgtgacg aacggacctt cccgttccag cagcttcccc agggggcggc cgatgccgtc
     2881 gaagatcgag cccatcaggc cgggccccag cgacatcgag agcggtcctc ccgttccggt
     2941 caccggctcg cccgtccgga gccccgtcgt atcctcgaag acctggacaa gggcgtcgtc
     3001 cccgtccaga cggatgatct ctcccaggag caggagccgt cccgcctgca ccatctcccg
     3061 catggcgaag gagcgcattc ccgacgccct gatcacgggg ccgttgatcg cgacgatctt
     3121 tccggtgctg ttcatctgcc gcccctcaca gcgccgcgag aagccgttcc gcgagagagt
     3181 ccttcagctc cgcggcgact tcgcgcagat ccgcagcggc acgccacgag ccgtccggag
     3241 cggaaagcca cgcgccgccg gaaatgggaa ccggcgacgg gtcgaaggtg aacgtcatgt
     3301 cggggcgctt cttcccgacc cgttccgcaa gggcgtcgcc cagaggggcg tcgtcgaccg
     3361 cgagcgaaat cagcgccgcc gttccggccg gaagatcctt cgccgcctcc agcaccagcc
     3421 ccgcgagcac cagcgggtag tcctcccgct cgcgcagcga ggtcagcgcc ctctgcagca
     3481 tcgccatcgc ctcgtcgagc agcgtgttct gaagacgaag gcgatccttc gcccgcagcg
     3541 actcggcccg cgcgatctgc cgtttcgcga tctcctccgc gttcttcacc gcatcggcgt
     3601 gaatctgctc caccatgcgg tccagccctt cgttctgctc cttcagccac gccgtcgctt
     3661 cccgccgcgc cgaatccagg atgacctccc gttcctcgtc gcccttcttc agcagcaggt
     3721 tcttcagtgc gagcagcttg cggttggaat cgtcccgaac gggcgtccgc tgcatctcga
     3781 gaagattctg ctcctcggcc gcccgcttgg cgggattctg cagctcccgc ccccttccgt
     3841 ctccggaaat ggaattgctc attctatctt cactccaatc gcgttgcgaa tgtaccgcgt
     3901 cagaaaatca ggttccctca ggctgccgtg cctgtcggga atttccacca ggagcggcgg
     3961 gtcctctctc tcccggagcg atttgaccag aaggggaacc tcggcggccg cgcgttccgt
     4021 catgaccagc acccccgtat ccgggcggtt gcgcaggacg tcgatcaccg cggtcgtcgt
     4081 ctcctcctct ccgtggacca ctacgcccgg aatgccggca agacgcaaca gcaccagaga
     4141 gtcgtggtta tcgctgatga gaaagccttt catcgccggc gccgcgttac agcttgccca
     4201 gaatcagaag ggagacgatg actccgtaga tggcgattcc ttcggcaagg ccgaggtaga
     4261 tcagcgtcgt acccagcatg gagggctttt cgccgaccac gccgagcgcc gccgcgccga
     4321 cgatcgccac ggcgagtccc gctcccaggc aggccattcc cgtcgcgagg ctcgcgccga
     4381 gaaagcccag cccggccccc tcgaccgccg ccgccccttc ggccgcggcg gcgctccctg
     4441 cggaaagggc g
//
LOCUS       DAOTIL010000108         4405 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminobacteriaceae bacterium isolate AMR_MDS_3026
            NODE_23529_length_4405_cov_4.123218, whole genome shotgun sequence.
ACCESSION   DAOTIL010000108 DAOTIL010000000
VERSION     DAOTIL010000108.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868050
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminobacteriaceae bacterium (wastewater metagenome)
  ORGANISM  Aminobacteriaceae bacterium
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae.
REFERENCE   1  (bases 1 to 4405)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4405)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:19:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,224
            CDSs (total)                      :: 3,198
            Genes (coding)                    :: 3,184
            CDSs (with protein)               :: 3,184
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4405
                     /organism="Aminobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23529_length_4405_cov_4.123218"
                     /isolate="AMR_MDS_3026"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:3034609"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..761)
                     /locus_tag="P5201_03365"
     CDS             complement(<1..761)
                     /locus_tag="P5201_03365"
                     /EC_number="6.3.4.15"
                     /inference="COORDINATES: protein motif:HMM:TIGR00121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004077 - biotin-[acetyl-CoA-carboxylase]
                     ligase activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biotin--[acetyl-CoA-carboxylase] ligase"
                     /protein_id="HRV97600.1"
                     /translation="MMKARPGTVDLCRILMNTGGEVISGGTLAGLLSCSRQSVWKSIR
                     SLVEEGFSIESIPQKGYILRELPRYDLAPSYIGALMNNTVFSKTEIYLYETLSSTQVT
                     AKQHGRQSGTENVVVISEEQTQGRGRRDRSWISPHGSGLFLSFFFKPRLLPGRLQLVN
                     LAAGLAVKDAVEKTCSVRLALKWPNDLLWENRKVCGILSEASSESDRIRDCCTGIGIN
                     ITSPSLENNREAAAVLSQAAFLNDMTGGVHRGVLAA"
     gene            853..1954
                     /gene="prfB"
                     /locus_tag="P5201_03370"
                     /pseudo
     CDS             853..1954
                     /gene="prfB"
                     /locus_tag="P5201_03370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014163239.1"
                     /note="programmed frameshift; internal stop; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology.
                     GO_function: GO:0003747 - translation release factor
                     activity [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 2"
     gene            1983..3839
                     /locus_tag="P5201_03375"
     CDS             1983..3839
                     /locus_tag="P5201_03375"
                     /inference="COORDINATES: protein motif:HMM:NF017400.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpoIVB peptidase S55 domain-containing protein"
                     /protein_id="HRV97601.1"
                     /translation="MNLFRLLICALVLLLAPAEQSEASRFTPSVPVLGVENLRPGMKG
                     YALTVVSGREIVRFPVEIVSVIPGKGSPKHLIMIKASGSVIAATGGIAAGMSGSPVFI
                     NDKLIGAIGYGWNFSEHTLGLVTSFSDMASIWSWPEKIVSIPPAPALDDADKKKDQED
                     ESQAGQEADNGKEDKEEDKLVVPVAAEQLSSPLFINGISTRKALETAALLGRDNFIPG
                     GGFTDAVPVEMNATMLPGDAITVLLAWGDVTIGATGTLTAVAPDGRFIAFAHPFLGRG
                     AVNYPVARAYIHKVIPSLESPFKVGSPLQIVGTVTQDRPQGIGGRIGYFTPSISATLD
                     FTDAERNIKERKRFHVVPDPFIGAKLTAGVFTGLIDDLWGRKGQGTATLTLNVQGRGL
                     ISGWTRTNMFFSDSDLAAAALKETSELLDVVFLNPFAEVYPLGITVTTEFSPMPKLMY
                     IEGVNLKGESFSSGETVEVEVIIRPYRKKQVKKSFTITIPKDASGICEILVRGGGIEP
                     LNQSAIVQGWKTIENFRQLFTEMSALETNNELIIEFNYEKRSKDGKGRSDEDEPVSKE
                     EQELLSELKQRRLKDGTLQIFKSEFVVEGLLRKLITVTPGNSSQSDEPETES"
     gene            3844..>4405
                     /gene="tmk"
                     /locus_tag="P5201_03380"
     CDS             3844..>4405
                     /gene="tmk"
                     /locus_tag="P5201_03380"
                     /EC_number="2.7.4.9"
                     /inference="COORDINATES: protein motif:HMM:TIGR00041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004798 - thymidylate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTMP kinase"
                     /protein_id="HRV97602.1"
                     /translation="MFITFEGIDGCGKTTQAALLANFLEGKYRGNRRVVWTREPGGWD
                     GGSWIRERILEGDFSHPMSELFLFLVDRCEHVRKEILPVLEGGGVVICERYTDSTLAY
                     QSWGRGIPSEKVETLFEWCSFPRPHKTFWLDVPVKTAMERVCLRGGRDRFEAEGYSFL
                     ERVREGFSSLAKREPQRICTVEASSEP"
BASE COUNT         1147 a         1030 c         1192 g         1036 t
ORIGIN      
        1 gccgccaaga ctccacggtg aaccccgccg gtcatatcat ttaaaaaagc tgcttgggaa
       61 aggacagccg ccgcctctcg gttgttttca agagacggag acgttatatt aattcctatt
      121 cctgtgcaac agtcacggat acgatcagac tcactggatg cttctgaaag aataccgcac
      181 acctttctat tctcccagag gagatcgttt ggccatttga gcgcaagcct tacggaacat
      241 gttttttcca ctgcgtcttt cacagcaagc ccggcggcga gattgacaag ttgtagtctt
      301 ccgggaagaa gacgaggttt aaaaaagaac gagagaaaaa gaccgcttcc gtgcggagaa
      361 atccagcttc tgtctcttct tcctctcccc tgtgtctgtt cttcggaaat gacgacgaca
      421 ttctccgttc cgctctggcg gccatgttgt tttgcagtta cctgtgttga ggaaagcgtt
      481 tcatagaggt agatttccgt tttagaaaaa accgtattat tcatgagcgc gccaatatac
      541 gagggagcaa gatcatagcg aggcaattca cgaaggatat atcctttttg agggatggac
      601 tctattgaaa agccttcttc aacgaggctg cgaatactct tccagacgct ctgccgggaa
      661 catgagagca gcccggcgag agtacctcct gaaatgactt cgccacctgt gttcatgaga
      721 atacggcaga ggtccacggt tccggggcgc gctttcatca tttcctttgt tcttcctcct
      781 ttggcatgcc tcgaaatgat ataataaaaa atcgttcaat acaatgatcg tcaccactaa
      841 ggaggagaaa gcatggcgct cacgccgaat gcgacagttt tggaggaact gaggatttta
      901 cgtaaagatc ttcaggacag tctttgacct cgattcaata agacagaaaa aagaggaact
      961 tgaagcgcgt acaactgctc cggatttctg gacaaaagag gaaagccgtg aagtatcccg
     1021 tgatatcgca cgtttacagc gcagcctgga ttcctgggat caggcggagc acatgttcag
     1081 cgacctcgaa actcttgatg agatgctctc ggaagatgat gacgatgagt tacagaggga
     1141 atttacgcag acggcggatg cgcttcggaa atttgtcgat aaagagcaga tggtgctgct
     1201 tctttccgag gagtatgaca gcagcaacgc aatactcaca gtacatgccg gatccggcgg
     1261 gctcgattct caggattggg ccgagatgct tttgcgcctt taccttcgat ggtctgaaag
     1321 agaaggcttt tccgcgaaag tgcttgatat ccttcaggac gaggaggctg gcattaaaag
     1381 cgcaaccgta cttgtgcagg gagagtatgc ttacgggtac ttaaaagcgg aaaagggagt
     1441 ccaccgatta gtacgaatat ctccattcga tgccgcaaag agacgacata ccagctttgc
     1501 atcggttgat gtcgctcccg aattggctga ggatgttccc gtggaaatcc gcccggaaga
     1561 cctcaaagtc gatacgttcc gggcaagcgg agccggcggt cagtatgtca acaggacgga
     1621 ttcggcggtc cgtattacac atattcccac cgggatcgtc gttgggtgtc agaacgaaag
     1681 atcccagcac atgaatcgtc aggttgcgat gcaggtcctt cggagccgtc tctttgaaaa
     1741 ggcgactcag gagcgccagc aagaacttct ctcgattcag ggagagaaga aggagataag
     1801 ctgggggagc cagatacgtt cctatgttct gcatccttat actattgtta aggatcatcg
     1861 gaccggcttt gaggtcggaa atgttcactc cgtgcttgat ggagacattg acgggttcat
     1921 tatgagcttc ctcagatgga agcgacaaaa gtaataaaat ttactcggga ggatcgccgc
     1981 ccatgaactt atttcgccta ctgatatgcg cgcttgttct tcttctcgct cctgcggagc
     2041 agtccgaagc gtcgcggttt actccctccg tcccggtgct gggggtagaa aatcttcgcc
     2101 ccggaatgaa ggggtacgcg cttaccgtcg tttccggcag agagatcgtg cgcttccctg
     2161 ttgaaattgt aagcgtgatc ccgggaaaag gaagccctaa acacctgata atgattaagg
     2221 cttccggctc tgtaatcgcg gcgacaggcg gaattgccgc aggcatgagc ggcagtcccg
     2281 tatttatcaa cgacaagctg atcggggcga ttggatatgg ctggaatttc agtgaacaca
     2341 cgctcggatt ggtcacctct ttcagcgata tggcaagcat ctggagttgg ccggaaaaaa
     2401 tcgtgtccat tcctccagcc ccggcacttg atgatgcaga taagaaaaaa gatcaggaag
     2461 acgagagtca ggcaggtcaa gaagcggata acggaaagga agacaaagaa gaggataaac
     2521 ttgtagtacc ggtagccgca gagcagctct cctcgcctct ctttatcaat ggtatcagca
     2581 ctcgcaaggc ccttgaaact gccgcccttc tcggaaggga caattttatt ccgggcggcg
     2641 gattcaccga tgctgttccg gtcgagatga acgccacaat gctgcccggg gacgccatta
     2701 cagtgcttct ggcatgggga gatgtaacca tcggtgctac cggcacatta acagctgtgg
     2761 cgcctgacgg aaggtttatc gccttcgctc accctttcct cgggagaggg gctgtaaact
     2821 atcctgtggc aagggcatac atccataaag taatccccag tttagagtct ccgtttaaag
     2881 tagggagccc gctgcaaatt gtcgggacgg ttactcagga cagaccgcag ggaatagggg
     2941 gaagaatcgg atattttaca ccttccattt cagcaactct cgattttacg gatgctgaac
     3001 ggaatataaa agaaaggaag cgttttcacg tcgttccgga tcctttcata ggcgcaaagc
     3061 tgactgcggg agtctttacc gggctgatcg acgatctgtg gggacgaaag ggacaaggta
     3121 cggcgaccct cacccttaat gtgcagggaa gagggttgat aagcggatgg acgaggacaa
     3181 atatgttctt ttcagacagc gatcttgcag cggccgcttt aaaagagaca agcgagctgc
     3241 tcgacgttgt gttcttgaac ccttttgccg aagtgtatcc cctggggata actgtaacta
     3301 ccgaattctc cccgatgcca aagcttatgt atatagaagg cgtgaatctc aaaggagaat
     3361 cgttctcctc cggagaaact gtcgaagttg aagttattat tcgcccttac aggaagaaac
     3421 aggtaaagaa atcatttacg attaccatcc cgaaggatgc ttccggtatc tgtgaaattc
     3481 ttgttcgagg cggcgggata gaaccgctga accaatcggc aatagtccaa ggctggaaaa
     3541 ccattgaaaa tttccgtcag cttttcaccg agatgtccgc acttgagacg aacaatgaac
     3601 ttatcatcga atttaattac gagaaacggt caaaagacgg aaaaggccgt tccgatgaag
     3661 acgagcctgt ttcaaaagaa gaacaagagc ttctgagcga gctgaaacag cgccgtttaa
     3721 aagatgggac gcttcagata ttcaaatcgg aatttgtcgt cgaggggctt cttcgaaaac
     3781 tgatcactgt cacgcccggc aactcatcgc aatcagacga gccggaaacg gagagttaat
     3841 cagatgttta tcacattcga aggtattgac ggatgcggaa aaacaacgca agctgcactc
     3901 cttgccaatt tccttgaggg aaaataccgg ggaaaccggc gcgtagtatg gacaagagaa
     3961 cccggagggt gggacggcgg ctcgtggata cgggaacgca ttttggaagg agatttttct
     4021 catccaatga gcgagttgtt tctctttctt gtggaccgtt gcgagcacgt tcgcaaggag
     4081 attctgcccg tacttgaggg cggcggcgtc gttatttgcg aacggtacac agactctaca
     4141 ctggcatacc aatcctgggg aagaggaatt ccgtcggaaa aggtagagac gctgttcgaa
     4201 tggtgttctt tcccgaggcc gcataagaca ttctggcttg atgtccctgt aaaaactgcg
     4261 atggagagag tctgcctccg cggaggacgc gaccgtttcg aggcggaagg gtattcgttt
     4321 cttgagaggg ttcgcgaggg attttcttcc ttggccaaga gagagcctca aaggatttgc
     4381 acggtcgagg cttcttcaga accgc
//
LOCUS       DAOTIL010000109         4396 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminobacteriaceae bacterium isolate AMR_MDS_3026
            NODE_23597_length_4396_cov_3.147431, whole genome shotgun sequence.
ACCESSION   DAOTIL010000109 DAOTIL010000000
VERSION     DAOTIL010000109.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868050
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminobacteriaceae bacterium (wastewater metagenome)
  ORGANISM  Aminobacteriaceae bacterium
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae.
REFERENCE   1  (bases 1 to 4396)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4396)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:19:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,224
            CDSs (total)                      :: 3,198
            Genes (coding)                    :: 3,184
            CDSs (with protein)               :: 3,184
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4396
                     /organism="Aminobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23597_length_4396_cov_3.147431"
                     /isolate="AMR_MDS_3026"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:3034609"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..568
                     /locus_tag="P5201_03385"
     CDS             <1..568
                     /locus_tag="P5201_03385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013049174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="flavoprotein oxidoreductase"
                     /protein_id="HRV97603.1"
                     /translation="AIASDEAMATSHPAVWAAGDCASTTHLMTGKPFWVALGTVANKM
                     GRVAGISMGGGRAFLKGVLGTAMSKHCAYEVARTGLSTREAEDAGFAFQSAVIKTKTR
                     AGYYPGAESMHVKLLGEEGTGRLLGGQIVGGQGSAKRVDIIATALAAGMTVHDLIDLD
                     LGYAPPFSMAWDPVQIAGRELMKKVGGE"
     gene            718..2121
                     /gene="pepV"
                     /locus_tag="P5201_03390"
     CDS             718..2121
                     /gene="pepV"
                     /locus_tag="P5201_03390"
                     /EC_number="3.4.13.-"
                     /inference="COORDINATES: protein motif:HMM:NF005591.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0016805 - dipeptidase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptidase PepV"
                     /protein_id="HRV97604.1"
                     /translation="MLSQKIDAMKDELIAALQESVRIPSVGGDPEEGAPFGAGPRKSL
                     DWTLAFAEKMGFRTKNVENVAGWAEMGEGDEMVAVLGHLDVVPEGKGWTVDPWGGELK
                     DGMIYGRGVLDNKGPVMVGLFAAKAIFDSGIALKRRIRVIFGTNEERGSKCMKRYVEV
                     GEELPAAGFTPDAEYPIIYAEKGILTYTLSLPFAPSGEMKLLSLEGGVAANVVMAEVK
                     AEITDPRPECRQRIMDAAAAWKGPEGSSLSAAEDAAQHIVTLEMKGHPAHGSTPEKGI
                     NALACLADFMNGLRLKGEQGDFFARFSKLIGFETNGASLAVAMNDDVSGALTACVGVV
                     KIANGRATFTLNIRYPVTKKEKEITGPLLETFAQNGITVESATSANPLYMPPESRLIK
                     ALQKAYTEVTGEEAELLAIGGGTYAKTMPNVVAFGPVFPGQTYKIHEEDECWSVEDIM
                     KNAHIMAKVLVELAERK"
     gene            complement(2230..3381)
                     /locus_tag="P5201_03395"
     CDS             complement(2230..3381)
                     /locus_tag="P5201_03395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014162849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HRV97605.1"
                     /translation="MKKAPWILAVLLVATLVTGSCAEEVIKVGYLAGITGDFAAYGQP
                     EVNAVKLAADEINAKGGVLGKKIELVIYDYKSRPEDAVNAARRLIDHDKVVAILGSAA
                     SGANIATAPLVTRAGIPQIGTLSTNPLVTVDESGNVRPYMFRICFIDPYQGRLIGYFA
                     AKELGKKKAAILYDVGSDYSHGLREHFISSYEEFGGTVVAEHAFRGGQDVDFRAQLTQ
                     IKQTDAEILVLPNIGKDLALPIKQARELGMEDIIFIGGDGYGEFMVEIAGEALEGSYW
                     MYHLARQDPAMKPFFDAYKEKYNDECQEFAIAVLAYDSLYWLVDAIERAGSADSKAIR
                     DALEATKDLQLHHATLTMDPKTHNPVDKDAVVLTVQGGKSIFYKKIKPE"
     gene            3597..>4396
                     /locus_tag="P5201_03400"
     CDS             3597..>4396
                     /locus_tag="P5201_03400"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF021127.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0045227 - capsule polysaccharide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CapA family protein"
                     /protein_id="HRV97606.1"
                     /translation="MNAHASPVLLPGRGSERRNEIRRWCFLVLSVLLLFLSCAGRAVA
                     AAGLLPAEHAEESGSPEKISRFSFLAVGDALIHKGVYEAARKGKKQFDFRPMFRLVKE
                     RVQAHDVAFYNQETILGGVDLRLSTYPCFNSPQEVGDGMIDAGFNLVSLANNHTLDRG
                     EKAILASHEYWAGQDGVLTAGSYASLEDRETPRLIEVNGITCAFLAYTTLTNGLKAPK
                     DKEFYVALYDKDSVEADVKRAREAKTDVVIVSIHWGSEYVFTPGAQQK"
BASE COUNT          906 a         1261 c         1346 g          883 t
ORIGIN      
        1 cgccatcgcg tctgacgagg ctatggcgac ttcgcacccc gccgtctggg cggccggaga
       61 ctgcgcctcg acgacgcatc tcatgaccgg aaaacccttc tgggtggccc tcgggaccgt
      121 ggcgaacaag atgggacgcg ttgccggtat ctccatggga ggcggccggg ctttcctgaa
      181 gggcgtgctc ggcaccgcca tgagcaagca ctgcgcgtac gaagtcgcgc gcaccggcct
      241 ttcgactcgg gaggcggagg acgccggatt cgccttccag tccgcggtca tcaagacgaa
      301 gaccagggcc ggatactacc ccggcgcgga gagcatgcac gtcaagctgc tcggtgaaga
      361 gggaactgga cgccttctcg ggggccagat cgtcggcggt cagggatccg cgaaacgggt
      421 cgacatcatc gcaacggccc ttgcggccgg aatgacggta cacgacctta tcgatctcga
      481 cctcggctat gcgccgccct tttcgatggc atgggatccg gtgcagattg ccgggcgtga
      541 actgatgaag aaggtcggag gggagtagcg cattcagaaa agcaatccgc ctgagcgctt
      601 tcccgatgag ttgagggtac gctcccgcac ttcgcagatc gtatgtgcga ggttttccgg
      661 gagcgctctc tttttatgaa tgttgtataa tagatatcat ttttaggagg aattgtcgtg
      721 ctctctcaga aaatcgatgc aatgaaagac gaactgatcg ccgcgcttca ggaaagcgtt
      781 cgtatcccaa gcgtaggagg ggatcccgag gaaggcgctc cattcggcgc cgggccccgt
      841 aagtcgctcg actggacgct tgcgttcgcc gagaagatgg ggttcaggac gaaaaacgtg
      901 gagaacgtcg ccggatgggc ggagatgggc gagggcgacg agatggtcgc agtgctcggg
      961 catctcgacg tcgttcccga ggggaaggga tggacagtcg atccctgggg cggcgaactg
     1021 aaggacggca tgatctacgg caggggcgtt ctcgacaaca agggacccgt catggtgggc
     1081 cttttcgccg cgaaggcgat cttcgactcg ggcatcgcgc tgaagcgccg aatccgcgtg
     1141 atcttcggaa cgaacgagga gcggggaagc aagtgcatga aacgctacgt cgaggtcgga
     1201 gaggaactgc ctgcggccgg attcaccccg gatgcagagt accccatcat ctatgccgag
     1261 aagggaattc ttacgtacac gctctctctt ccgttcgcac cgtcaggcga gatgaagctg
     1321 ctctctctcg aaggcggcgt ggcggcaaac gtcgtcatgg cggaagtcaa ggcggagata
     1381 accgaccctc gtcccgagtg cagacaacgc atcatggacg cggcggccgc ctggaaaggc
     1441 cccgagggga gttcgctcag cgcggcggag gatgctgcgc aacatatcgt gacccttgag
     1501 atgaagggcc atcccgccca cgggagcacc cctgagaagg gcatcaacgc gctcgcctgc
     1561 ctcgccgact tcatgaacgg tctcagactg aaaggggagc agggggattt cttcgcccgc
     1621 ttctcgaagc ttataggctt cgaaaccaac ggcgcctcgc ttgccgttgc gatgaacgac
     1681 gacgtctcgg gcgcgctcac cgcatgcgtc ggcgtggtga agatcgcaaa cggacgtgcg
     1741 acttttaccc tcaacatccg ctaccccgta acgaaaaaag agaaggagat taccggacct
     1801 cttcttgaga catttgcgca gaacggtatc actgtcgaaa gcgcaacctc ggccaatcct
     1861 ctctacatgc ctcccgagtc ccgcctcatc aaggcgctgc agaaagccta caccgaggtg
     1921 accggcgaag aggccgagct gctggccatc ggcggcggca cctacgccaa aaccatgccg
     1981 aacgtcgtgg ccttcggccc cgtcttcccc gggcagacct acaaaattca cgaggaagac
     2041 gagtgctggt cggtggagga tatcatgaag aacgctcata tcatggcgaa agttctcgtc
     2101 gaactcgccg agaggaagta acctcgctcg cagggtggcc gtagtacaca gcacgcgcac
     2161 aaagagggga gagccggtat tcccgggttc tcccctctcc ttgttcatgc actgctgccg
     2221 agtctggcgt tattccggct tgatcttttt gtagaatatg ctctttccgc cctggacggt
     2281 gagcacgacg gcgtccttgt ccacggggtt gtgggtcttc gggtccatcg tcaacgtcgc
     2341 atggtgaagc tgcagatcct tcgttgcttc aagggcgtcg cggatggcct ttgaatccgc
     2401 gcttccggcg cgctcgatcg cgtcgacgag ccaatagagg ctgtcgtagg cgagcacggc
     2461 gatggcgaac tcctggcact cgtcgttgta cttctcttta taagcgtcga agaagggctt
     2521 cattgccggg tcctgacggg cgagatggta catccagtac gaaccctcaa gagcctcccc
     2581 agcgatctcg accatgaact cgccgtaacc gtcgccgccg atgaagatga tgtcttccat
     2641 tccaagctcc ctcgcctgct tgatcggaag cgccaggtcc ttcccgatgt tcggaagaac
     2701 cagtatttcg gcgtccgtct gcttgatctg cgtcaactgg gcccggaagt cgacgtcctg
     2761 gccgccgcgg aacgcgtgct ccgcgacaac cgtgccgccg aactcttcgt agctgctgat
     2821 gaagtgctcg cgcaggccgt gggagtagtc gctaccaacg tcgtagagga tcgcggcctt
     2881 tttcttaccg agctccttgg ctgcaaagta tccgatcaga cgcccctggt agggatcgat
     2941 gaagcagata cggaacatgt acggacggac atttccgctc tcgtcgacgg tgacaagcgg
     3001 gttagtggag agcgtgccga tctgcggaat tccggcgcgc gtgacgagcg gcgcggtcgc
     3061 gatattggcg ccgctggccg cggaaccgag aatcgcaaca accttgtcgt ggtcgatcag
     3121 tcttctggcg gcgttgaccg catcttcggg acgggatttg tagtcgtaga tgaccagctc
     3181 gatcttcttg ccgagaacgc cgccctttgc gttgatctcg tccgccgcga gcttgaccgc
     3241 gttcacttcc ggctgaccgt acgctgcaaa gtctcctgtg attcctgcaa gataccctac
     3301 cttgataact tcttcagcac acgaacctgt tacgagggtt gctactaaaa gtaccgcgag
     3361 aatccatggc gcctttttca tgaagcactc acctccggga attattgcgg ttatgcgaaa
     3421 agaagagctc cgcacgaccg atccgatata gtatccgata ttcgatatgc taatctctga
     3481 gactctcttt gtcaatggag aagttcgata agttggattc gcaacggaag agaagcctat
     3541 ttctccgttc atgagggtat aatagacata gagcttttgc cggaggtggt cctttgatga
     3601 atgcacacgc ttcccccgtc ctgttgccgg ggagagggag cgaacgccga aatgaaatcc
     3661 ggcgatggtg ttttctcgtt ctctcagttc tccttctctt tctttcgtgc gcgggacggg
     3721 cggttgccgc cgccggtctt cttcctgcag agcacgcgga ggagagcggg agccctgaga
     3781 aaatctcacg cttttccttc ctcgccgtcg gcgacgcgtt gattcataaa ggcgtgtacg
     3841 aagcggcgcg aaaggggaaa aaacagtttg atttccgccc gatgttccgg cttgtaaaag
     3901 aacgggttca ggcgcacgac gttgccttct acaaccagga gactatcctc ggcggcgttg
     3961 acttgcgcct gtccacgtac ccgtgtttca actctccgca ggaagtgggc gacggtatga
     4021 tcgacgccgg cttcaacctc gtctcgctgg cgaataacca cacgcttgac cggggggaga
     4081 aggcgatcct cgcctcgcac gaatactggg cggggcagga cggagtcctg acggccggaa
     4141 gctatgcgtc gctggaggac cgggaaacgc cgaggctcat cgaggtaaac ggcatcacct
     4201 gcgccttcct cgcgtacacc acgctcacga acggtcttaa ggcgcccaag gacaaggagt
     4261 tctacgtagc cctctacgac aaggattcag ttgaggcgga cgtgaagcgg gccagggagg
     4321 cgaaaacgga tgtcgtcatc gtctccattc actggggaag cgaatacgtc tttacgcccg
     4381 gagcgcagca gaagga
//
LOCUS       DAOTIL010000110         4387 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminobacteriaceae bacterium isolate AMR_MDS_3026
            NODE_23660_length_4387_cov_3.438596, whole genome shotgun sequence.
ACCESSION   DAOTIL010000110 DAOTIL010000000
VERSION     DAOTIL010000110.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868050
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminobacteriaceae bacterium (wastewater metagenome)
  ORGANISM  Aminobacteriaceae bacterium
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae.
REFERENCE   1  (bases 1 to 4387)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4387)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:19:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,224
            CDSs (total)                      :: 3,198
            Genes (coding)                    :: 3,184
            CDSs (with protein)               :: 3,184
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4387
                     /organism="Aminobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23660_length_4387_cov_3.438596"
                     /isolate="AMR_MDS_3026"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:3034609"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..550
                     /locus_tag="P5201_03405"
     CDS             <1..550
                     /locus_tag="P5201_03405"
                     /inference="COORDINATES: protein motif:HMM:NF024700.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HRV97607.1"
                     /translation="PGHDGADLVPFLYYLREMDRDRYEVVLDALKTAFPDFEELNFPP
                     VAAGMLAMTWKDKNFSKPMYMNELSEGTLRFLWLASLLQSPHLSTITMIDEPEVSLHP
                     ELLSLLPDLMREASKRTQIVVTTHSDRLIRFLKPEEVVVMDLDEEGCASATWADSMDI
                     DRWLAEYSLDEVWRMGRMGGRS"
     gene            547..1194
                     /locus_tag="P5201_03410"
     CDS             547..1194
                     /locus_tag="P5201_03410"
                     /inference="COORDINATES: protein motif:HMM:NF025468.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4276 family protein"
                     /protein_id="HRV97608.1"
                     /translation="MKIAIIVEGKTEKAFLPHLRAFLKNCLAGETPKLDVLPYDGRIP
                     KEDKLRRVVENLLGGSKPADHVIALTDVYTGGTSPDFLDAQDAKKKMRQWVGAEPRFH
                     PHEISALHRFHPHVAQYDFEAWLLPYWPTIQKLARHNKGAPGGNPETVNHHNPPAYRI
                     KEIFEIGKCRESYVKPRDAARILRENDLSVAVAQCSELKAMINTILSLCGGPVIP"
     gene            1401..1847
                     /locus_tag="P5201_03415"
     CDS             1401..1847
                     /locus_tag="P5201_03415"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRV97609.1"
                     /translation="MTKEELGAVYRDFLAEEGFSPFIEEDGDVTFKYEGKVHGISVDE
                     GDEEFFRLIFAPFWDIENEEERRKVHAAACEATAKVKVAKIFPLGDDVVGSVELFLPE
                     VDDFKPVFKRALRCLSAAVDAFVASMRRGEKAIVVIEDGAGASVDS"
     gene            2040..3101
                     /locus_tag="P5201_03420"
     CDS             2040..3101
                     /locus_tag="P5201_03420"
                     /inference="COORDINATES: protein motif:HMM:NF016629.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rpn family recombination-promoting
                     nuclease/putative transposase"
                     /protein_id="HRV97610.1"
                     /translation="MADKDNAFRRMLGDKEMFLRFLRRFLRRDMPAVVEDLIESGDFS
                     LDDLILENITFIPPDLREKRSDVVYRIRRGTLEAYVYILIEHQSSVDFLMPYRMLSYM
                     VRLWDRCIEEAGESARRKSFQLPPIMPVVFYDGERPWTAVKLFTHKVRNTEDFRGYVP
                     DFEYRLISLRDVAANDLLRPGDALGTLLYLAKPFKSENFFEVSERIRKFLLVFPDEEK
                     LLLARHLGGYLKMLAKREGLDADSAPEDMFENEEAENMLTYLEKEIRRYKREGREEGL
                     AEGREEGRQEGWREGRQEGRQEGREEGRQEGRQEGREEVLRQTVRRMLASGMAVSLVA
                     EILEFSEERVRQLAGENSD"
     gene            3210..3950
                     /locus_tag="P5201_03425"
     CDS             3210..3950
                     /locus_tag="P5201_03425"
                     /inference="COORDINATES: protein motif:HMM:NF019349.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HRV97611.1"
                     /translation="MEFLHRLGGESSREWAMRVVLQNIIHLVLPPGTPLSDKEIASFL
                     SLSRTPVREALVLLEEMRFVDVYPQKGTFVSLLDVDDIEEGRYIRKCLESDTFIQACR
                     SFSADGAIALEANLKMQQNALDTGDRKRFLFLDQDFHRLICSGCGRERVWSVISKNSL
                     HFFRVRRLKIKGGGVGQGYFHEQHGELLDAILAHETDRALDILNRHLTWDVETVRASY
                     PDYFTRDSMSKKMYMFMSEVAPVVAGAM"
     gene            3979..>4387
                     /locus_tag="P5201_03430"
     CDS             3979..>4387
                     /locus_tag="P5201_03430"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRV97612.1"
                     /translation="MKKTCQPVVTEMRVIPVAGRDSMLLNLSGAHGPYFTRNVVLLTD
                     SEGRTGVGEVPGGEKIAGGLEQARDLVVGSRTGEYKNTLLAVKKLFAGREDDVRGQQT
                     FDLRTGVHVLTGIEAPLLDLLGQYLGVPAASLLG"
BASE COUNT          979 a         1105 c         1368 g          935 t
ORIGIN      
        1 gccggggcat gacggtgcag atcttgttcc gtttttgtat tacctgcgcg agatggatcg
       61 tgatcgttac gaggttgttc tggacgcatt gaaaacggcc ttcccggatt tcgaggagct
      121 gaattttccg ccggtggcgg ccggaatgct ggcgatgacg tggaaagaca agaatttcag
      181 caagccgatg tacatgaacg aactctccga aggaactctt cgattcctct ggcttgcgtc
      241 gctgctgcaa agccctcatc tttcaaccat caccatgatc gacgaacccg aagtcagtct
      301 gcacccggaa ttgctgagtc tgctgccgga tttgatgcgc gaggcctcca agcgaacgca
      361 aatcgttgtt accacccatt cggacaggtt gatacgtttt ctaaaacccg aagaggtggt
      421 ggtgatggac ctggacgaag aaggctgcgc atcggcgaca tgggccgatt ccatggatat
      481 cgaccgatgg ctggccgaat acagccttga cgaagtgtgg cggatggggc gaatgggggg
      541 ccggtcatga aaatagccat catcgtcgaa ggcaaaaccg aaaaggcgtt tcttccccac
      601 ctccgtgcct ttttgaaaaa ctgtctggct ggcgaaacgc ccaagttgga tgttctaccc
      661 tacgacgggc gcatccccaa agaagataaa ttacgacggg tcgtggaaaa ccttttggga
      721 ggaagtaaac ccgctgacca tgtgattgcg ctgaccgatg tttataccgg cggaacgtct
      781 cccgattttc tcgacgctca ggacgccaag aaaaaaatga gacagtgggt gggtgcagag
      841 ccgagatttc atccccatga aatctcggct ctgcacagat ttcatcccca tgtcgcccaa
      901 tatgatttcg aggcgtggct gttgccgtac tggccgacga ttcaaaagtt ggcaagacat
      961 aacaaaggcg cccccggagg caatcccgaa acggtgaacc atcacaatcc gcctgcatac
     1021 cgaatcaagg aaatcttcga gatcggcaag tgcagggaaa gctatgtcaa gcctcgcgat
     1081 gcagcgcgga ttttaaggga aaacgacctc tccgtcgccg tcgcccaatg ctccgaactc
     1141 aaggcaatga tcaacacgat tctttctctg tgcggaggcc cggtcatccc atgaacatgg
     1201 gcgtttctcc ggttgctgaa atactggagt cgttttgttg ctttcgatca acatctttct
     1261 ggaaataccc tctaaacggt cgcctccggt cggtatgatg gttggtgtgg agagagttcg
     1321 tcctgatcgg atgcgaagct tcaccaaaac ggctgtgata ttcggtattt cggacggtat
     1381 cggagaggag cgttgttgcc atgacgaaag aagagcttgg tgcggtgtat cgggattttc
     1441 tggccgaaga ggggttctct ccctttatag aagaagatgg agatgttacg ttcaaatacg
     1501 aaggaaaagt tcatggcata tcggtcgacg agggcgacga agagtttttc cgtctgattt
     1561 tcgctccctt ttgggacatc gaaaacgagg aggaacggcg aaaagtgcac gccgccgcct
     1621 gcgaggccac ggcgaaggtg aaggtcgcca agatttttcc tcttggagac gacgtcgtcg
     1681 gaagcgtcga actgtttttg ccggaggtcg atgacttcaa gcccgtgttc aagagggctt
     1741 tgcgctgctt gtcggcggcg gtcgacgcgt tcgttgcgtc gatgagaaga ggtgaaaagg
     1801 cgatagtcgt gatagaggac ggcgccgggg cgtccgtcga ctcctgagaa tatccgtccg
     1861 cgtggaagct gctcaagctc gcaaaagcag cttccacgga caaggaatcg gacaggtatg
     1921 cggcgcgggg cctgccgcga atccaggaat ttgcgtgata gcggatggta ccggtgaaga
     1981 agagggcttt tctccacatg ctataataat caaaaatttc atcgtcgagg gagagctgta
     2041 tggctgacaa ggacaacgct ttcaggagaa tgctcgggga caaggagatg ttcctccggt
     2101 ttctccgccg ttttttacgg cgcgatatgc ctgcggtcgt cgaggatctg atagagtccg
     2161 gagatttttc tctcgacgat cttatcctgg agaatatcac gttcatcccg ccggatctcc
     2221 gggagaagcg gtcggacgtc gtctatcgca tccggcgtgg aacgcttgaa gcgtacgtct
     2281 acatactgat agaacatcag tcgagcgtcg actttctgat gccgtatcga atgctgtcct
     2341 acatggtgcg cctgtgggat cgctgcatag aagaggcggg ggagagcgcc aggaggaaaa
     2401 gcttccagct tcctccgatc atgcccgtgg tcttttacga cggggagagg ccatggacgg
     2461 cggtgaagct gtttacgcat aaggtgcgaa acaccgagga ttttcgcgga tacgttcccg
     2521 atttcgagta tcgtctgatc tccctccgcg acgtggccgc caatgatttg ctgcggccgg
     2581 gtgacgcgct gggaacgctt ctctatctgg cgaagccgtt caaaagcgag aactttttcg
     2641 aggtgtccga gaggatacgg aagtttctgc tggtctttcc ggacgaagaa aagctgctcc
     2701 tggcgagaca cctcggcggt tatttgaaga tgctggcgaa gcgagagggc ttggatgccg
     2761 atagcgcgcc ggaggatatg ttcgaaaacg aggaggctga gaacatgctg acctacttgg
     2821 agaaagagat acggagatat aagcgcgagg gccgcgagga agggttggcg gaagggcgcg
     2881 aagaaggacg gcaggagggg tggcgagaag gacgtcaaga aggacgtcaa gaaggacgtg
     2941 aagaaggtcg tcaggaaggt cgtcaagaag gacgtgaaga agtccttcga cagaccgtcc
     3001 ggcgcatgct tgcctcgggc atggccgttt ctctggtcgc agaaatactg gagttctccg
     3061 aagagcgggt gcgtcagctc gccggggaga attcggactg atttcgagcg tctcaggagg
     3121 aggcattatc ctgagagatt tttcttgaca aagccgcaac tgatatatta gtatgactag
     3181 catataaaac taggatgagg agggggagca tggagttttt acaccgtctc ggcggcgagt
     3241 cgtccaggga gtgggcgatg cgcgtcgttc tccagaacat cattcatctt gtccttccgc
     3301 cggggacgcc gctctccgac aaggagatcg cctcctttct ttcactgagc cggacacccg
     3361 tccgcgaagc tcttgtcctt cttgaagaga tgcgttttgt cgacgtctac cctcagaagg
     3421 ggacgttcgt ctccctgctc gacgtggacg atatcgagga ggggcgctac atccgcaagt
     3481 gcctcgaatc cgatacgttt atccaggcgt gccgctcttt ttccgccgac ggggcgatcg
     3541 cgctcgaagc caatctgaag atgcagcaga acgcgctgga tacgggcgac cgcaagcgtt
     3601 ttctctttct cgaccaggac ttccaccggc tgatctgcag cgggtgcggc cgcgaaagag
     3661 tctggtcggt catcagcaag aattcgctgc actttttccg cgtccgcagg ctgaagatca
     3721 agggcggcgg cgtcgggcag gggtatttcc acgagcagca cggggagctt cttgacgcga
     3781 tactcgcgca cgagaccgat cgtgcgctgg atatcctcaa caggcacctc acttgggacg
     3841 tggagacggt gcgcgcctcc taccctgatt atttcacgcg ggattccatg tcgaagaaga
     3901 tgtacatgtt catgtccgaa gtcgcgcccg tcgtcgcggg tgcgatgtag cgcattgacg
     3961 aatggaaagg acgacagcat gaagaaaacg tgccaaccgg ttgtaacgga aatgcgggtg
     4021 atccccgtcg cggggcggga cagcatgctc ctcaacctca gcggagccca cggtccgtac
     4081 ttcacgcgca acgtggtgct gctgacggac agcgagggga ggacgggcgt cggagaggtt
     4141 ccggggggcg agaagatcgc cggggggctc gaacaggccc gcgacctcgt cgtcggctca
     4201 cggacggggg agtacaagaa cacgctgctc gccgtgaaga agctcttcgc ggggcgggag
     4261 gacgatgtgc ggggacagca gacattcgac ctgaggacag gggtgcatgt actcaccggc
     4321 atcgaggcgc cgctgctcga tctgctgggg cagtacctcg gcgtgcccgc ggcgtcgctc
     4381 ctcggcg
//
LOCUS       DAOTIL010000111         4367 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminobacteriaceae bacterium isolate AMR_MDS_3026
            NODE_23808_length_4367_cov_3.848098, whole genome shotgun sequence.
ACCESSION   DAOTIL010000111 DAOTIL010000000
VERSION     DAOTIL010000111.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868050
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminobacteriaceae bacterium (wastewater metagenome)
  ORGANISM  Aminobacteriaceae bacterium
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae.
REFERENCE   1  (bases 1 to 4367)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4367)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:19:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,224
            CDSs (total)                      :: 3,198
            Genes (coding)                    :: 3,184
            CDSs (with protein)               :: 3,184
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4367
                     /organism="Aminobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23808_length_4367_cov_3.848098"
                     /isolate="AMR_MDS_3026"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:3034609"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(65..1816)
                     /locus_tag="P5201_03435"
     CDS             complement(65..1816)
                     /locus_tag="P5201_03435"
                     /inference="COORDINATES: protein motif:HMM:NF015983.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HRV97613.1"
                     /translation="MDLLLIKSHRSVCGYIPGSNLGMNILIQLLRQKKYTAEMFQGYA
                     KEALSHVKMLMEQEDRPKVIGFYCDFDNVHWVRLFAREVKRNYPEVILLAGGPQTVDM
                     DADFLKDTGILAVCIGEGEETVPELMEYCIHRRGSLETIQGIAFLDASSQLVKTPPRT
                     PPEALDSIPWPDITLTNDYKHYSLLPILTGRGCPFGCTFCYEGANAKRVRRHSVPSLL
                     HEIRANFERNPSIKYINFLDDTFTLDHKRVDEICEGIKELRKEYDFVWFASAHISLIG
                     KHRDMARTMAEAGLKKIFFGLESGSDAVLQSYNKKITRKMAVEVIAHCVDSGIHAISG
                     NIILGGPHETRETVQESEDLIMELLYRAPGQFETAYFSFLPFPQTPITLNPQKFGMEI
                     YESLIDCCNEDIPLSGTEALDFIELTKIRLQANKRLQNEMRTIYLDGKIPESVILDAY
                     RLGERYGVFTRWNDYVYKNLPIDDAYWRMRAFGEYVTGSQLGAWEENAIPHRTFELWL
                     YTDISGAKPRVVDTPLEEIDYTLLKLCSGKLSKREVIRQGKAALDPAGTQPDFYKRAE
                     EALHSMEQKKWILYQKP"
     gene            complement(1901..2164)
                     /locus_tag="P5201_03440"
     CDS             complement(1901..2164)
                     /locus_tag="P5201_03440"
                     /inference="COORDINATES: protein motif:HMM:TIGR03798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Nif11-like leader peptide family RiPP precursor"
                     /protein_id="HRV97614.1"
                     /translation="MDGAKALAAKVLTDEELAKKLGEAKSEAEFNAVVAKLGYKCTAA
                     EFEAAFKEAQGKAPLSDEQLDQASGGLSIVGVDYAFVAVQTAK"
     gene            2496..3740
                     /locus_tag="P5201_03445"
     CDS             2496..3740
                     /locus_tag="P5201_03445"
                     /inference="COORDINATES: protein motif:HMM:NF015316.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein transport protein"
                     /protein_id="HRV97615.1"
                     /translation="MRHSLRILSVSFLLLFLTSSSLFGAGFALYDFSARGNALGGAMV
                     GRADDPSAIAFNPAGITQIPGSAFMTGLGFLLPRGTAVAAGTAMAADQNDRTFILPNM
                     YYTKQMNEKLWLGVGVMTRFGLGTEFPHDWFGRYNSYRAMLESVSINPNIAWKVNDAL
                     SLSVGVEALWFEADLRRKIDSTQGVAPNPATDIDLRLKGDDIGYGWNVGLHYKMDETT
                     RFGLHYRSRVKLALSGTAIATSPLATDSTEGRVDLTLPDMLMFGVSKQLTPKLNVEVG
                     AIHTGWDSYDSIAINFGKPLLGALPIRSFTPKNWASVWRYQLGLEYAYSPEWTWRLGY
                     TYDQEPMPDATIDYQLPTADRQLFSIGFGYAKNDWALDFAYSRLLSDERTIAGRRAEG
                     ILDSRTHDMNVNIFMLSYSIRF"
     gene            3929..4315
                     /locus_tag="P5201_03450"
     CDS             3929..4315
                     /locus_tag="P5201_03450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011916063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HRV97616.1"
                     /translation="MNSQTSVSSDEALRLTPDEVSSRMPDERTLYELADLFRVFGDST
                     RIKILCALSVSDLCVADIASLLSMTQSAISHQLRVLKQVRLVRVARKGRSSVYSLDDD
                     HVRTIFDQGFRHVRHIAPSSERGEAK"
BASE COUNT          922 a         1198 c         1160 g         1087 t
ORIGIN      
        1 atttcaaagg tcctccccgg gaccagccgt tgggtgtaca cgaggagaac aaggggagac
       61 gcgttcatgg tttctggtag agaatccatt tcttctgttc catcgaatgc agcgcttctt
      121 ccgcccgttt gtaaaagtcg ggctgcgttc ccgcggggtc gagcgccgct ttcccctgcc
      181 gaatgacttc ccgtttcgag agcttcccgc tgcataattt gaggagcgtg tagtcgattt
      241 cttccagcgg cgtatcgaca acccgcggtt ttgccccgct gatgtcggtg tacagccaca
      301 actcgaaggt ccggtgcgga atggcgttct cttcccaagc gcccagttga ctgcctgtga
      361 catactcgcc gaacgcccgc attctccagt aggcgtcgtc gatgggcagg tttttgtata
      421 catagtcgtt ccatcgggta aatacgccgt agcgctcccc cagacggtat gcgtccagaa
      481 tcaccgattc gggaattttg ccgtcaaggt agattgttcg catttcattc tggagcctct
      541 tgttggcctg aaggcgtatc tttgtaagct ctatgaaatc caatgcttcc gttccggaaa
      601 gcggaatatc ttcgttgcag cagtcgatga gggactcgta aatttccatc ccgaattttt
      661 gaggattcag ggtgatcggc gtttgaggaa acggaagaaa ggagaagtag gcggtttcaa
      721 attgcccggg cgccctatac agcagctcca tgatcagatc ttcggactcc tgaacggttt
      781 ccctggtttc gtgaggccct cccagaataa tgttgccgga aatagcgtga atccccgagt
      841 cgacgcagtg ggcgatgacc tccaccgcca tcttccgcgt aattttcttg ttatagcttt
      901 gcaaaacggc gtcgctgccg gactccaggc cgaaaaaaat ttttttcagc cccgcctccg
      961 ccatagtgcg ggccatgtcc cggtgtttgc cgatgaggga gatatgagcg ctggcaaacc
     1021 atacgaaatc gtactctttg cggagttcct tgattccctc gcagatttca tcgacccgtt
     1081 tatgatccag cgtaaaggtg tcgtcgagaa aattgatgta cttaatggag gggttccgtt
     1141 caaagtttgc cctgatctcg tgaagcagcg agggaacgga gtggcggcgc acccgttttg
     1201 cattggcccc ctcatagcag aaggtgcatc cgaaaggaca gccccgtccg gttaaaatgg
     1261 ggagaagcga gtagtgttta tagtcgttgg tcaaggtgat atcaggccaa ggaatggaat
     1321 ccaaggcttc aggcggggtt ctcgggggtg ttttcacaag ctgtgaagaa gcatccagaa
     1381 aagctattcc ctgaattgtc tccagtgaac ctcggcgatg gatacaatac tccatgagct
     1441 ccggaactgt ttcttcccct tcgccgatgc agacggcaag gattcccgtg tctttcaaaa
     1501 aatcggcgtc catgtcgacg gtttgcggcc cgcctgccag cagaatcact tccgggtagt
     1561 ttcttttcac ttcccgggcg aagagcctga cccagtggac attatcaaaa tcgcaataga
     1621 atccgatgac tttgggccgg tcttcctgct ccatgagcat ttttacatgg ctcaaggctt
     1681 ctttggcata gccctgaaac atctcggctg tatatttttt ttgccgtaaa agctgtatta
     1741 aaatattcat tcccagattg cttccgggaa tgtacccgca gacagagcgg tgtgatttta
     1801 tgagcaacag atccacgtgg tttcctccgc ctaaaagagg agaccggaaa aattccggcc
     1861 tcctctttca tcatccgtga aggagaaggg gctccttcgt ttattttgct gtttgcacag
     1921 ctacaaacgc gtaatccacg ccgactatgc tcaaaccgcc tgaagcctga tccaactgct
     1981 cgtcgctgag cggcgccttc ccctgggctt ccttgaaggc agcctcgaat tccgccgcag
     2041 tacacttgta ccccagtttt gcaaccaccg cattgaattc cgcttcggat ttcgcctctc
     2101 ctagtttctt cgccagctcc tcgtccgtta gaacctttgc agccaatgct ttcgcgccgt
     2161 ccatgatttt cttctcctcc ttgtgtgtct ataaggtagg tgatacgcac ctcgtatgaa
     2221 aatatagaaa ttattataca gcccaccggg atattgtcaa atgagctctt tggagagatt
     2281 tttgctgaag ttgcgatgcg ctcgaaacat ggaagatcgg cgtcgctgtg gcggcgatac
     2341 cgtctgttcc attgaatgaa ctcctcttga gacaagttgt ttcaaatgtc accgacaggt
     2401 aacaaaaatc tcttccgcgt gatataatgc gaacgacttt tttccgcaag gagttttctg
     2461 caaacataat ctcctgacaa aaggagtggt ataggttgcg acattctctt cgtatcctca
     2521 gcgtttcgtt tcttcttctt tttcttacca gttcgtcgct cttcggggcg ggattcgcgc
     2581 tgtatgactt cagcgcgcgc ggcaacgcgc tcggcggggc tatggtgggc cgggcggacg
     2641 atccttcggc aatcgcgttc aacccggccg gtatcacgca gatccccgga agcgcgttca
     2701 tgaccggtct cggcttcttg ctgccccgcg gaacggctgt cgcagcggga accgcaatgg
     2761 ctgcggatca gaacgacaga acctttatac tgccgaacat gtactacaca aagcagatga
     2821 acgaaaagct ctggctggga gttggcgtga tgacacggtt cgggctcggg acggagttcc
     2881 ctcacgactg gttcggacgc tacaacagct accgggcgat gcttgaatcg gtctcgatca
     2941 acccgaacat cgcctggaag gtcaacgatg cgctctcgct ctcggtgggg gtggaagccc
     3001 tctggttcga ggcggatctg cggcggaaaa tcgactcgac acagggcgtt gcccccaatc
     3061 cggcgacgga catagacctt cgcttgaaag gggacgacat agggtacggg tggaacgtgg
     3121 gactgcacta caaaatggac gagaccacgc ggttcggcct tcactaccgg agcagggtga
     3181 agctcgccct gtcgggaacc gccatagcaa cgagtccgtt ggccaccgac tcgacggaag
     3241 gccgcgtgga ccttaccctt ccagatatgt tgatgttcgg cgtcagcaag cagctgaccc
     3301 ccaagctcaa cgtggaggta ggcgccatcc ataccgggtg ggacagctac gactccatcg
     3361 cgatcaactt cgggaaaccc ttgctcgggg cgctgcccat ccgctctttc acgccgaaga
     3421 actgggccag tgtctggaga taccagctgg ggctcgagta tgcgtattct cccgagtgga
     3481 cctggaggct tgggtacacg tacgaccagg aaccgatgcc ggatgcgacc atcgactatc
     3541 aactgcccac cgccgacaga cagctctttt ctataggctt cggatatgcg aagaacgact
     3601 gggcgcttga ttttgcctac tctcgactgc tctccgacga gagaaccatc gccggacgcc
     3661 gggcggaggg gattcttgac tcgcggacac atgacatgaa cgtcaatatc ttcatgctga
     3721 gctacagcat caggttttaa cctctattcc cggagggcgc gggcccgctt cgtcgctctc
     3781 cgggattttt ttgtctcgcc tcctacattc ttttcatgct tgtgtattct gtctttttgc
     3841 tcttttctcc tccctcttgc gggtagagtc tttttatgat atatgaatag ttgctcatat
     3901 attcatataa acatataagg aggcccccat gaactctcaa acatctgttt cgtctgatga
     3961 agcgcttcgc ctgaccccgg atgaagtatc ctcccggatg cccgacgaaa ggacgctcta
     4021 cgagcttgca gatctcttcc gggtgttcgg cgacagcacc aggataaaaa ttctctgcgc
     4081 gctttccgtt tccgatctct gcgtcgcgga tatcgcgtcc ctgctttcca tgacccaatc
     4141 tgcaatttcg caccagcttc gggtgcttaa acaggtgcgt cttgtccgcg tcgccaggaa
     4201 aggacgatcg tccgtgtaca gccttgacga cgaccacgtg cggaccatct tcgaccaggg
     4261 attccggcat gtgcggcaca ttgcgccctc ctctgaaagg ggggaggcga agtgagcgtt
     4321 tcacgaagcg aattgcgtct ctcgctggag gggctgaact gcgccca
//
LOCUS       DAOTIL010000112         4366 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminobacteriaceae bacterium isolate AMR_MDS_3026
            NODE_23814_length_4366_cov_3.521921, whole genome shotgun sequence.
ACCESSION   DAOTIL010000112 DAOTIL010000000
VERSION     DAOTIL010000112.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868050
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminobacteriaceae bacterium (wastewater metagenome)
  ORGANISM  Aminobacteriaceae bacterium
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae.
REFERENCE   1  (bases 1 to 4366)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4366)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:19:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,224
            CDSs (total)                      :: 3,198
            Genes (coding)                    :: 3,184
            CDSs (with protein)               :: 3,184
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4366
                     /organism="Aminobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23814_length_4366_cov_3.521921"
                     /isolate="AMR_MDS_3026"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:3034609"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..742
                     /locus_tag="P5201_03455"
     CDS             <1..742
                     /locus_tag="P5201_03455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005660563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="EF-Tu/IF-2/RF-3 family GTPase"
                     /protein_id="HRV97617.1"
                     /translation="LSRTVAPEEEAFSGFVFKIQANMDPSHRDRLAFMRVCSGKFERG
                     MKMRHHRIGKDVTVANPVIFMAQERALAEEAWPGDIIGIHNHGTIKIGDTFTDKEPLK
                     FTGIPSFAPEHFRRVRLQSPLKAKQLQKGLLQLTEEGAVQLFRPLGRNDYILGAVGVL
                     QFDVTMARLKDEYGVEATYEPVNYETARWVTCADEKRLAEFQKANQNDLAIDGEGNLA
                     FLADGEWRLRHVSEKWPGIEFHTTRELR"
     gene            766..1176
                     /locus_tag="P5201_03460"
     CDS             766..1176
                     /locus_tag="P5201_03460"
                     /inference="COORDINATES: protein motif:HMM:NF027567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase family
                     protein"
                     /protein_id="HRV97618.1"
                     /translation="MEKTILDLAGELLAKSEVVTLASVTEEGFPRVCAMARVRSEGVK
                     TVWMATGTYSKKTAHFRKNPKASVCAYSGGDSLTLVGNVAVLTDEQTRKELWQEWFIK
                     HFPKGPDDPDYCVLKFDAVEATVYINEVFETHRV"
     gene            1188..1736
                     /locus_tag="P5201_03465"
     CDS             1188..1736
                     /locus_tag="P5201_03465"
                     /inference="COORDINATES: protein motif:HMM:NF013492.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HRV97619.1"
                     /translation="MFRPVLILGDPLLRRKSAPIGDFSDPALKDEMRDLKEALERFRR
                     EHGFGRGIAAPQIGIPKRMIALNLGQGTFVIANPEIVRRSDQTFTMWDDCMSFPDLLV
                     RVRRHVSIDLHYLDEDGAAKEWTGIGQAESELLQHEIDHLDGVMATDRAIEPEDILYK
                     SEYQKHKKLYDERVDYVITPTI"
     assembly_gap    1886..1985
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <1986..2235
                     /locus_tag="P5201_03470"
     CDS             <1986..2235
                     /locus_tag="P5201_03470"
                     /inference="COORDINATES: protein motif:HMM:NF021474.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="type II toxin-antitoxin system VapB family
                     antitoxin"
                     /protein_id="HRV97620.1"
                     /translation="LIARGVRVYYNVYEVMCMRTNIVIDDELMEEAFTVSGGVSTKKE
                     LVELALREFVDTRKRRDLRELRGRVAFADEYDYKSMRQ"
     gene            2238..2645
                     /locus_tag="P5201_03475"
     CDS             2238..2645
                     /locus_tag="P5201_03475"
                     /inference="COORDINATES: protein motif:HMM:NF013970.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIN domain nuclease"
                     /protein_id="HRV97621.1"
                     /translation="MILVDTSVLVGYLKGSAGAVFDRFDEMIAGGVPFGINGYIYQEV
                     LQGARDEREYALLAEYLSSLPFYSLLHGRASFESAARIFFLCRRSGVTVRSTVDLLIA
                     QTAIENELYLFHNDRDFSNMAKIAPELKLIDYF"
     gene            2720..3364
                     /locus_tag="P5201_03480"
     CDS             2720..3364
                     /locus_tag="P5201_03480"
                     /inference="COORDINATES: protein motif:HMM:NF012523.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CatA-like O-acetyltransferase"
                     /protein_id="HRV97622.1"
                     /translation="MEEIRMETWDRREHFEFFLRNDLPFYNVNFNADITGLREYAKGA
                     SVSLSAALIYLVTKSLNRVPNFLYRVLGGKVVRYERLHPQFTCLRGDEELFRLVTVEF
                     CDDLAEFDRSVKRAIEESDAYFNVGMLAHRSDFVFISSLPWIPFTGIDHTLSLNKEDA
                     IPRVSWGRFFEQGGRTLLPCNIRVNHIFIDGLHVGRFYETFQEETRRLADSSPR"
     gene            complement(3392..3790)
                     /locus_tag="P5201_03485"
     CDS             complement(3392..3790)
                     /locus_tag="P5201_03485"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system VapC family
                     toxin"
                     /protein_id="HRV97623.1"
                     /translation="MIVVVDASAAVEISLAKPSANRFREELLDAALTLAPDTYASEIT
                     NVFWKYRAYSGLPEQACLEGIEFCLGIVDEYIPSVELWREAFFEASKAKHPVYDMLYL
                     IVARRHNARLLTKDGKLAELASQSGIFVVS"
     gene            complement(3787..4032)
                     /locus_tag="P5201_03490"
     CDS             complement(3787..4032)
                     /locus_tag="P5201_03490"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRV97624.1"
                     /translation="MPTLQVRDLPEELYQKMLYLAEKEHRSLTQTTIVLLKEGLERRL
                     ADTERRRDLLRNFSGIGPDAAGLPDAEILVREDRDRR"
BASE COUNT          853 a         1155 c         1398 g          860 t
ORIGIN      
        1 gctttcgcgc accgtggctc cggaggagga ggcgttctcc ggctttgtct tcaagattca
       61 ggcgaatatg gacccgtcgc accgcgaccg gctggctttc atgcgcgtgt gctcgggcaa
      121 gttcgagcgg gggatgaaga tgcggcatca ccgcatcggc aaggatgtga cggtggccaa
      181 tccggtgatc ttcatggcgc aggagcgcgc gctggcggaa gaggcgtggc cgggcgacat
      241 catcggcatc cacaaccacg gcacgatcaa gatcggcgac acgttcacgg acaaggagcc
      301 gctgaagttc acgggcattc ccagcttcgc gccggagcac ttccggcggg tgcgcctcca
      361 gtcgccgctg aaggcgaagc agctccagaa ggggctgttg cagctgacgg aggagggggc
      421 ggtgcagctc ttccggcctc tggggcgcaa cgactacatc ctgggcgcgg tgggcgtgct
      481 ccagttcgac gtgacgatgg cgcggctgaa ggacgagtac ggcgtggagg cgacctacga
      541 gccggtgaac tacgagacgg cgcgctgggt gacctgcgcg gacgagaagc gccttgcgga
      601 gttccagaag gcgaaccaga acgacctcgc catcgacggc gaggggaacc tggccttcct
      661 ggcggacggc gagtggcgtc tgcgccacgt ctccgagaag tggccgggga tcgagttcca
      721 caccacgcgc gagctgcggt gagggatata aaggaggggg ccacgatgga gaagacgatt
      781 cttgatcttg ccggggagct gctggcgaag tcggaggtgg tgacgctggc ctcggttacg
      841 gaggaggggt ttccccgcgt ctgcgcgatg gcgagggtgc gctccgaggg cgtcaagacg
      901 gtgtggatgg ccaccggcac gtattcgaaa aagacggcgc acttccggaa gaacccgaag
      961 gcgagcgtct gcgcgtattc gggcggcgac agcctgacgc tggtggggaa cgtcgccgtg
     1021 ctgacggacg agcagacgag gaaagagctg tggcaggagt ggttcatcaa gcacttcccg
     1081 aaggggccgg acgatccgga ctactgcgtt ttgaagttcg acgccgttga ggcgaccgtc
     1141 tatatcaacg aagtgttcga gacgcaccgc gtataggagg cggaaaaatg ttcagacccg
     1201 tgctgattct cggcgaccct cttctgcggc ggaagtccgc gccgatcggc gacttttccg
     1261 atcccgcgct gaaggacgag atgcgcgacc tcaaggaggc gctggagcga tttcgccgcg
     1321 agcacggctt cggacggggc atcgccgcgc cgcagatcgg cattccgaag cggatgatcg
     1381 cgctgaacct cggacaggga acgttcgtga tcgcgaaccc cgagatcgtc cgccgaagcg
     1441 accagacgtt caccatgtgg gacgactgca tgtccttccc cgatctgctg gtgcgggtga
     1501 ggcggcacgt gtcgatcgac cttcactacc tcgacgagga cggcgccgcg aaggagtgga
     1561 cgggcatcgg ccaggccgag tcggagctgc tgcagcacga gatcgaccac ctggacggcg
     1621 tcatggcgac cgaccgggcc atcgagccgg aggacattct ctacaagtcg gagtaccaaa
     1681 agcacaagaa gctctacgac gagcgtgtcg actacgtcat aacgccgacg atctgaaccc
     1741 ggagaagcgc gctgataggt gggagcgcga tctactctct ttgcagcatg tggaataatc
     1801 gacgcgccct gaacaaccgg acagttccgt cctgattgct cggggcgttc gtgtgtatta
     1861 taatgtgtat gaggtgatgt gtatgnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1921 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1981 nnnnncctga ttgctcgggg cgttcgtgtg tattataatg tgtatgaggt gatgtgtatg
     2041 cgcacgaata tcgtcatcga tgacgaactg atggaggagg ccttcactgt ttccggcggc
     2101 gtttcgacga aaaaggagct tgtggaactc gccctgaggg agttcgtgga cacgagaaag
     2161 agaagggatc tccgcgagct tcgggggcgg gtcgcctttg cggacgagta cgactacaag
     2221 tccatgaggc agtgagtatg atcctggtgg atacgtctgt ccttgtgggg tacctcaaag
     2281 ggagcgcggg cgccgttttc gacaggttcg acgaaatgat cgccggcggc gttccgtttg
     2341 gaatcaacgg gtacatctat caggaggtgc ttcagggggc gagggatgaa agagagtatg
     2401 ctctccttgc cgaatatctg tcttctttgc ctttctattc gctgcttcac gggcgggcgt
     2461 cgttcgagtc agcagcccgt atcttctttc tgtgcaggcg ttccggcgtc accgtgcgca
     2521 gcaccgtcga tttgctgatc gctcagaccg ccatcgaaaa cgaactgtat ctctttcaca
     2581 acgacagaga tttttcaaac atggcgaaga ttgcccccga actcaagctg atagactatt
     2641 tttagcgctt cgacagaatt ccgtcgaagt tttttgacgt gtcgttcttg cgtgtcgggg
     2701 gcgtccggag gaggtttgca tggaagagat tcgtatggag acgtgggaca gacgagagca
     2761 tttcgagttc ttcctgagga acgatctccc cttttacaac gtgaacttca acgcggatat
     2821 caccggcctg agggagtatg cgaagggggc ttccgtctcg ctgagcgccg cgttgatcta
     2881 cctggtgacg aagtcgctga accgtgtgcc gaacttcctc tacagggtgc tcggcggcaa
     2941 ggttgtgcgg tacgagcgcc tgcaccctca gttcacctgt cttcgaggcg acgaggaact
     3001 cttccggctg gtcactgtgg agttttgcga cgaccttgcg gagttcgacc ggagcgtcaa
     3061 gcgcgcgatc gaggagagcg acgcctattt caacgtgggg atgctggcgc acaggagcga
     3121 cttcgtcttc atctcctccc tgccgtggat accgtttacg gggatcgacc acacgctgag
     3181 cctgaacaag gaggacgcga ttccccgcgt gtcgtggggg cggttcttcg agcagggcgg
     3241 gcggacgctg ctgccgtgca atatccgggt gaatcatatc ttcatcgacg ggcttcatgt
     3301 ggggcgtttc tacgagacgt ttcaggaaga aacgcgcagg ctggcggact cgtcgccccg
     3361 ctgaggccgt tttccctcgt gagtcggctg tttacgaaac gacgaagatt ccgctttgag
     3421 aggcgagttc ggcgagcttt ccgtccttcg tgagcaggcg ggcgttatgc cgccgcgcaa
     3481 cgatgagata gagcatgtcg tacacggggt gcttcgcctt cgatgcctcg aaaaacgcct
     3541 cccgccagag ctcaaccgag gggatgtact cgtcgacgat gccgaggcag aattcgattc
     3601 cctccagaca cgcctgctcg ggaagcccgg agtatgcgcg gtacttccag aagacgttcg
     3661 ttatttcgga ggcgtaggta tccggcgcga gcgtcagcgc ggcgtcgaga agttcctcgc
     3721 ggaaccggtt cgccgaaggt tttgcgaggg agatctccac tgcggcgctt gcgtcgacga
     3781 cgacgatcat cgacggtccc tgtcctctcg gacgagaatc tccgcatccg gcagacctgc
     3841 ggcgtcgggg ccgattccgg aaaagttccg gagcagatcg cggcggcgct ccgtgtcggc
     3901 aaggcgccgt tcgagcccct ccttcagcag aacgatcgtc gtttgcgtaa ggcttcggtg
     3961 ctccttctcc gcaaggtaga gcattttttg atacagctct tcgggtaaat cccgaacctg
     4021 aagcgtcggc acgactccca cctccctttc atgcattcta gatgtttttt gcatgcgaat
     4081 caaggactcc tcggagcgcg cattgcaaca tggcgtgttt ccgaaggatt ctaagcgcac
     4141 gtcttcaagc gatatttcaa gtgtaccccg agggaatgaa tacagaaaaa gtcaccgtga
     4201 gtcgatacaa taggcggtgg gtgagctcat ccccgcagat ttttcgcaca gcgcaaaaga
     4261 aagaagagtt cgatcaatgg aaaaagaaaa cacgcagtat ctcaagatga tagggatgcc
     4321 ggtcggcaag ctgattctgt cgctgtcggt cccgacgatc gtcagc
//
LOCUS       DAOTIL010000113         4363 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminobacteriaceae bacterium isolate AMR_MDS_3026
            NODE_23838_length_4363_cov_3.485144, whole genome shotgun sequence.
ACCESSION   DAOTIL010000113 DAOTIL010000000
VERSION     DAOTIL010000113.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868050
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminobacteriaceae bacterium (wastewater metagenome)
  ORGANISM  Aminobacteriaceae bacterium
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae.
REFERENCE   1  (bases 1 to 4363)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4363)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:19:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,224
            CDSs (total)                      :: 3,198
            Genes (coding)                    :: 3,184
            CDSs (with protein)               :: 3,184
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4363
                     /organism="Aminobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23838_length_4363_cov_3.485144"
                     /isolate="AMR_MDS_3026"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:3034609"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            56..985
                     /locus_tag="P5201_03495"
     CDS             56..985
                     /locus_tag="P5201_03495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008521626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HRV97625.1"
                     /translation="MEILIPILTAAVRSGTPILYATLGEIVTEKGGILNLGLEGIMLL
                     GAYAGFAVSYSTGNAWLGIAAAFFVGTLLGLVHAFICVTLKGNQVVSGLAMTMFGTGA
                     SSLLGRNFVGFTIPGLNKIPVPLLSDIPVIGPVLFNHDALIYCSYALVFFLCWFFNST
                     RQGLYLRATGDNPRAADAMGISVAKTRYFYTMVGAGIVAVGGAYMSVAYTKMWAEHMT
                     AGRGWIAVALVIFAIWNPARAAFGAYLFGGVEAFQLRLQAAGTTIPAPILMMLPYLMT
                     IGVLLFISIKKGKGIVFGAPASLGLPFFREERD"
     gene            1094..1798
                     /locus_tag="P5201_03500"
     CDS             1094..1798
                     /locus_tag="P5201_03500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013047651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF554 domain-containing protein"
                     /protein_id="HRV97626.1"
                     /translation="MDFFFQHIPVSGSILNAAAIALGGLAGLLLHSRLSQRFITITFQ
                     GLGLFTIFLGFLMAQRTQSVLIMVFSIVGGSLLGEWFDLDKRLNNLAEWTKGKIRSKN
                     EKFTEGFVAASLLFCVGSMAILGAIEEGMGEYPRLFITKSFMDLMASAAMGASLGVGV
                     VFSAFPVLVYQGALTLLAEGVQRVMSPEVIAEVSAVGGLMLLGIGLAILEIKRIKVIN
                     MLPALILAGILASFFR"
     gene            complement(1887..2636)
                     /locus_tag="P5201_03505"
     CDS             complement(1887..2636)
                     /locus_tag="P5201_03505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014163669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M15 family metallopeptidase"
                     /protein_id="HRV97627.1"
                     /translation="MNHPFMDIPITAAPSFSWEASRAVPIDECGEEMVAASLIPEKIL
                     VRPQYYLHMLEGAIPECYVRRSVLERLIEAADMLPDGRRLVLLDTWRSRNIQTTLFQK
                     FRSELREKMPLLDDSEITVLASQFVAPPSLHPERPSPHITGGAVDLTIVDKMGFCLPM
                     GTGFDETSEKSATVWYEKKFDSKEAMSEDETEALHNRRLLYYVMHKAGFTNYPDEWWH
                     YDYGNQNWALLRGKKHAIYGVTEPAFRWRSL"
     gene            complement(2661..3950)
                     /locus_tag="P5201_03510"
     CDS             complement(2661..3950)
                     /locus_tag="P5201_03510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013048720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0031317 - tripartite ATP-independent
                     periplasmic transporter complex [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter large permease"
                     /protein_id="HRV97628.1"
                     /translation="MGISMLFAFLLLMILGVPLYLSLLSTSLFGIVMLGDFSLLRVMS
                     QQFFGGMDVFSLMAIPFFILAGILMNKTGLTDRLLDFSRLLVGSVRGGLGYVNVVGGI
                     ILAGVNGSAAADASALGSILIPAMVKDGFSPPYAAGLTAGSALIGPIIPPSIFMIIYA
                     TMTNTSIGGLFAAGILPGILLGGAFMGMNYVYSHRYQVPVADFEEVRKMKKEILKRSL
                     VALFAPVIIIGGIITGVVTPTESGALAVAYCLVAGTLYTRQMTFRVLAESLYETVRLT
                     SSIFLIMGAAMVIGWLLKWEQVPQKFASIILQAGLLDKPWLLMVVLSAIIFPVGMFME
                     EVSTLALLTPIFAPIALKAGIDPFHFGVVMTLNVTIALITPPMGACNYIVAAVGRVKL
                     SDVFFHIWPFIGVALLVLLAIISFPPLTTLIPRMLNL"
     gene            complement(3955..>4363)
                     /locus_tag="P5201_03515"
     CDS             complement(3955..>4363)
                     /locus_tag="P5201_03515"
                     /inference="COORDINATES: protein motif:HMM:NF016200.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="TRAP transporter small permease"
                     /protein_id="HRV97629.1"
                     /translation="LLAFNIGDILVGVFCRYVLKSSIIWTEEAARFSLVWMVMVGALG
                     AAEKGDHMVIDFVVPRFPAALRRLIGWGRFLLTILILSLMIYLGTVNAVQMWNMRTMA
                     LNIPKTIPLLSLPVGFLLLMAGTILMQFDRGRE"
BASE COUNT          977 a         1254 c         1202 g          930 t
ORIGIN      
        1 tccggaggcg cgactcctcc gatcgcaacg acctctgcaa aggaggagaa gtagcatgga
       61 aattctgatt cccattctca cagcggctgt ccgaagcggc acgcctattc tgtacgcgac
      121 tctcggcgag atcgtcacgg aaaaaggagg catcctcaac ctcggacttg aaggcatcat
      181 gctcctgggc gcgtatgcgg gattcgcagt ctcctactcc acggggaacg catggcttgg
      241 gatcgctgcg gcattcttcg ttggaacgct cctggggctt gttcacgctt tcatctgcgt
      301 cacgctgaaa ggcaaccagg tagtcagcgg tctcgccatg accatgttcg gtacgggcgc
      361 gtcctctctc ctgggccgca atttcgtcgg cttcaccatc ccggggttga acaagattcc
      421 cgttccgttg ctctccgaca tccctgtcat cggacctgtg ctcttcaatc acgatgcgct
      481 catctactgc tcgtacgccc tcgtcttctt tctctgctgg ttctttaact ccacaaggca
      541 ggggctgtat ctccgcgcca ccggagacaa ccctcgagcc gccgacgcga tgggcatctc
      601 cgtcgcgaag acgcgctact tctacacgat ggttggagcg ggtatcgtag ctgtgggcgg
      661 cgcgtacatg tccgtggcct atacgaagat gtgggcggag cacatgaccg ccggacgcgg
      721 atggattgca gtcgcgctcg tcatcttcgc catctggaac cctgcgcgcg cagccttcgg
      781 cgcctatctt ttcggcggtg tggaagcctt ccagctcaga cttcaggctg cgggaaccac
      841 tatccctgcg ccaatcctga tgatgctccc ctacctgatg acgatcggcg tccttctctt
      901 catctccatt aagaaaggga aagggatcgt cttcggagcg cctgcctccc tgggacttcc
      961 ttttttccgg gaggaacgcg actaactggt ttcccgaacg cacacacgct catcgggcgt
     1021 cggcgatcga tacggcgccc gatgacgacg gccgccggat atactctttc accatacagg
     1081 aggttgttcc aaagtggatt ttttctttca gcacatcccc gtttcgggga gcattctcaa
     1141 tgcggcggca atcgctctcg gcggcctcgc cggtcttttg ctccattccc gtctttcgca
     1201 acggtttatc accattactt ttcagggact gggactcttc acgatctttc tcgggtttct
     1261 gatggcgcag aggacgcaga gcgtcctcat catggtcttc agcatcgtcg gcggctcgct
     1321 tctcggcgaa tggttcgacc tggacaaacg tctgaacaac ctggcggagt ggaccaaggg
     1381 aaagatacgc tcgaaaaacg aaaagttcac agaggggttc gtcgccgctt cgcttctctt
     1441 ctgcgtcggc tcgatggcga tcctcggcgc catcgaagag gggatgggag agtatcccag
     1501 gctcttcatc acgaaatcct ttatggatct gatggcctcc gcggcgatgg gcgcctctct
     1561 cggagtcggg gttgtgtttt ccgccttccc tgtccttgtg taccagggag ccctgacatt
     1621 gcttgcggag ggcgtacagc gtgtgatgag cccggaggtg atcgcggagg tgagcgcggt
     1681 aggcgggctt atgctcctgg gcataggact cgcgatactg gagatcaagc gcatcaaggt
     1741 gatcaacatg cttcccgccc ttatattggc ggggatcctg gcgtcattct ttcgctgatc
     1801 cgcttttcgc tgacctgcta aaaagaaacg gaaggccgga gtatggattc tccggccttc
     1861 cgtttctttt ttagctcttc ttcgtatcag agggaacgcc agcggaacgc gggttctgtt
     1921 acgccgtaga tcgcatgttt cttcccgcgg agcagcgccc agttctgatt gccgtagtcg
     1981 tagtgccacc actcgtcggg gtagttggtg aatcccgcct tgtgcatcac gtaatacagc
     2041 agccgccggt tgtgcagggc ctccgtctca tcctcggaca tggcctcttt tgaatcgaat
     2101 tttttctcgt accacacagt ggccgacttc tcggaagtct cgtcgaaccc ggttcccatc
     2161 ggcagacaga agcccatttt atcgacgatg gtcaggtcta ccgctccccc cgtgatatga
     2221 ggcgaggggc gctcggggtg cagcgacggc ggcgcaacaa actgagaggc gagaacagta
     2281 atttccgaat catcaagaag cggcattttc tcacggagtt cggagcggaa tttctgaaaa
     2341 agggtcgtct ggatattgcg ggaacgccac gtatccaaga gcacaagccg gcgcccgtcg
     2401 ggcagcatat ctgctgcttc aataaggcgt tcgagcaccg aacggcgtac gtagcactcc
     2461 gggatggcgc cttcgagcat atggagatag tactggggcc gaacaaggat cttctccggt
     2521 ataaggcttg cggccaccat ctcctcgccg cactcatcta tcggcaccgc tctcgaagct
     2581 tcccacgaaa agcttggagc ggctgtaatc ggtatgtcca tgaagggatg attcattttt
     2641 cttctccttc catttccgtg ttacaggttc agcatccgcg gaataagcgt agtcagcgga
     2701 ggaaatgata tgatcgcgag cagaacaagc agggcgacgc cgatgaaagg ccagatgtgg
     2761 aaaaaaacgt ccgaaagctt caccctcccc acggctgcga cgatatagtt gcatgctccc
     2821 attgggggag tgatcagcgc aatcgttaca ttgagcgtca tcacaactcc gaagtgaaag
     2881 gggtctatgc ccgctttcag cgcgataggc gcaaaaatag gggtaagaag cgccaaagtt
     2941 gaaacctctt ccatgaacat gcccaccgga aagatgatgg cgctgagcac gaccataagg
     3001 agccacggtt tgtccagaag ccccgcctgg aggatgatag aagcaaactt ctgcggtact
     3061 tgctcccact ttaaaagcca tccgataacc atggccgctc ccatgatgag aaagatcgag
     3121 ctggtcagtc ggacggtctc gtagagcgac tccgcgagta cccggaacgt catctgccgc
     3181 gtgtacagcg ttcctgcgac caggcaatac gcaaccgcga gcgcgccgga ttccgtcggg
     3241 gtgacgaccc ctgtaataat tcctcctatg ataatcacgg gcgcgaaaag tgcgaccagc
     3301 gaacgtttca ggatctcttt tttcatttta cgaacttctt caaaatcggc gacgggaacc
     3361 tggtaccggt gagagtagac gtagttcatt cccataaaag ctcctcccag caggataccg
     3421 ggcaggatac cggcggcaaa cagcccgcct atcgacgtat tcgtcatggt agcgtagata
     3481 atcataaaaa tactcggagg gataatcgga ccgatgagcg cgctccccgc cgtcagcccc
     3541 gccgcgtagg gaggagagaa accgtctttg accatggcgg gaatcagaat ggaaccgagg
     3601 gcggacgcat ccgcggcggc agaaccgttc accccggcga ggatgatgcc gccgacgacg
     3661 ttgacgtacc ccaaaccgcc ccgaaccgac ccgacgagca gacggctgaa atcaagcagt
     3721 ctatccgtca accccgtctt gttcattaaa ataccggcaa ggatgaagaa cgggatagcc
     3781 attaaggaga acacgtccat accgccgaaa aactgctgac tcatgacacg caataaagaa
     3841 aaatcgccaa gcatcactat cccgaacaaa gaggttgaga gcagcgagag atagagcggc
     3901 acgccaagga tcatcaggag taaaaaagcg aaaagcatcg atatccccat aggatcactc
     3961 cctccctcgg tcgaactgca tcagtattgt acccgccatc aagagaagaa accctacagg
     4021 cagtgaaaga agcgggatcg tctttggtat attgagcgcc atcgttcgca tgttccacat
     4081 ctgcacagca ttgaccgtgc caagatagat catcagtgaa aggatgagta ttgtcaggag
     4141 aaagcgcccc cagccgataa gccgccggag ggcggcggga aaacgcggaa ccacaaaatc
     4201 gatgaccatg tgatcccctt tctccgccgc cccgagggct cccaccatga ccatccacac
     4261 cagagagaag cgcgcggcct cctccgtcca gattatggag cttttgagca cgtacctgca
     4321 gaacacgccg acgagaatat cgccgatgtt gaaggcaaga aga
//
LOCUS       DAOTIL010000114         4361 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminobacteriaceae bacterium isolate AMR_MDS_3026
            NODE_23854_length_4361_cov_3.830237, whole genome shotgun sequence.
ACCESSION   DAOTIL010000114 DAOTIL010000000
VERSION     DAOTIL010000114.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868050
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminobacteriaceae bacterium (wastewater metagenome)
  ORGANISM  Aminobacteriaceae bacterium
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae.
REFERENCE   1  (bases 1 to 4361)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4361)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:19:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,224
            CDSs (total)                      :: 3,198
            Genes (coding)                    :: 3,184
            CDSs (with protein)               :: 3,184
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4361
                     /organism="Aminobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23854_length_4361_cov_3.830237"
                     /isolate="AMR_MDS_3026"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:3034609"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..136)
                     /locus_tag="P5201_03520"
     CDS             complement(<1..136)
                     /locus_tag="P5201_03520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012870147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CtsR family transcriptional regulator"
                     /protein_id="HRV97630.1"
                     /translation="MASLTRVIENYINNLFSEMDEQTVSLRRKELAETFGCVPSQINY
                     V"
     gene            complement(565..1176)
                     /gene="plsY"
                     /locus_tag="P5201_03525"
     CDS             complement(565..1176)
                     /gene="plsY"
                     /locus_tag="P5201_03525"
                     /EC_number="2.3.1.15"
                     /inference="COORDINATES: protein motif:HMM:TIGR00023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0008374 - O-acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006654 - phosphatidic acid biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate 1-O-acyltransferase PlsY"
                     /protein_id="HRV97631.1"
                     /translation="MTALIWGTLGYLAGSFPTGYLAGLWVKGVDIRTIGSGGTGATNA
                     GRLLGKNWAKAVAIVDMLKGAVPMLCARWWGISDPWIIALIAFAGVVGHNYPVWLSFK
                     GGKGVATSYGVAFFLYPHLSFFVAPAGGLVWLLVLKAKGYVSLASMTSLCCLPFFALV
                     LAFPLPYVLVLGALALLAIFRHKDNIKRLREGKESRFGKHAKS"
     gene            complement(1173..2630)
                     /locus_tag="P5201_03530"
     CDS             complement(1173..2630)
                     /locus_tag="P5201_03530"
                     /inference="COORDINATES: protein motif:HMM:NF024938.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetate--CoA ligase family protein"
                     /protein_id="HRV97632.1"
                     /translation="MQPGVPGNTALLADGGDVLVRCLSAFQQRRAPLRYAFSFGEAVD
                     YSPLEAASSILQSDSQTDFYVFILKELREGRKFLDFASDAAKKGKRTAVLLLRRPQTS
                     SREETAALKQFGVVLLNDIQEIVDGALAFLALKNSDLSGGKECAYHTSSDELSMLIKK
                     EAERAEFTLQESARATSPLLIDVLSGSDSLNTVQRRKQRIEQSGRLPVFSSLADNALF
                     EEQLYSAKIPLIPGIRRCFNALQLVFGSGPAVCPAPSEGAHPTKCPLHPRPTEYDAKV
                     LLRAYDIPVAREALCHSLPEALTAAETIGYPIALKVMSPSILRKTEARVIALDVQNEE
                     ELKNAYGRTLEKARIANPSARIRGVLVQEMVHGGTEWRMEYRKDPRFGPVVEMSFSGI
                     FGEIFPDGVLRAAPFDTDEALRMIKESKGYPLLLAGWRRERLDVEAFASSLAAFSRLA
                     YCETEIARLDVNPVFVNRIGVLVVDAFIERRGGIR"
     gene            complement(3057..4139)
                     /locus_tag="P5201_03535"
     CDS             complement(3057..4139)
                     /locus_tag="P5201_03535"
                     /inference="COORDINATES: protein motif:HMM:NF016488.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RelA/SpoT domain-containing protein"
                     /protein_id="HRV97633.1"
                     /translation="MNATKVDELGKKYMRQYSLYLEFAARACNLLENLVEQDGIQLYN
                     VSGEAKTPEGFLRMYEALAEGVPSSLSEIADLCTVKILVHFPEDVEKVERIVQQEFLV
                     DMARSVTSASLDDPDRFGYPAVYYTLSLSRARAAMREWSKYKDIPFSLEIRTVLQEVW
                     AANTPKVMLPTDTSTKKKIQRKLFRVGALLEEADEGYFSLWESARESAMPVTPPVARV
                     EPVASTRRVFDRNALRQYFQEHEEIPRRWAEIASDVGFPPFRPDPEYLETSLSYLWDI
                     LRASEMDSPDEFQRFIASLEEEEKGREQVATVYKAFEREAQSWRVDGYSALFLLVLNL
                     KWDVLQNKDLVELNIKRGSDRIKGIL"
BASE COUNT         1180 a         1170 c         1021 g          990 t
ORIGIN      
        1 ggacatagtt gatctggctc gggacacacc cgaaagtctc cgcaagctcc tttctccgaa
       61 gtgaaactgt ctgttcatcc atttcggaga aaaggttatt tatgtagttt tcgatgaccc
      121 gcgtcaggct tgccatttta ttccctccac actgcaaatt ccatacaaac agcacgttac
      181 tgaactgtat aaccaatctg ctccggacaa tctatcccac gcataccttg ccgctcggcg
      241 gaaaacagat cctcgtttct ccacttgaag cagaaagagt gaaacaataa acgtatttcc
      301 ctcaaagata tggttcaatc tgcaaacaag tactcagatt gaacgcatct cttataggct
      361 attctacgat gttggtcagt attagtcaat aaggaggggt gtctgcctct accacaggtt
      421 ttaaagacag aagagcacaa ccggcgaaca gaaaacaact tcttgcccgc ttgagcagat
      481 agcattgacg atactctgcg caatctgccg cagagtactc cggagacgga cctaccgtct
      541 gcggattcga gttctttcag ttcttcagga ttttgcgtgt tttccgaagc ggctttcctt
      601 tccttctcgt aatcgcttga tattatcttt gtggcggaaa attgccagca aggcaagcgc
      661 gcccaacacc aaaacatacg gaagagggaa agcaagaacg agcgcaaaaa aaggcaggca
      721 acagagagag gtcattgaag ccaaagagac gtaacctttt gctttcagaa cgagcagcca
      781 taccaggccg cccgctgggg caacaaagaa agaaagatga ggatacaaga agaacgcgac
      841 gccataagat gtcgcgactc cctttcctcc tttgaaagag agccaaacag ggtagttgtg
      901 ccccacaacg ccggcaaaag caatgagcgc gataatccac gggtccgaaa taccccacca
      961 ccttgcgcaa agcattggaa ccgcgccttt caacatgtcc actatcgcaa cggccttcgc
     1021 ccagtttttg ccgagaagcc ttccggcatt cgtcgccccc gtcccccctg atcctatcgt
     1081 ccgaatatcc actcccttta cccacagacc cgccaggtag ccggtaggga aagaccccgc
     1141 aagatacccg agcgtccccc atatcaaagc agtcatcgaa ttccacctct cctttcaata
     1201 aaagcgtcta cgactaaaac accaatccga tttacgaaaa cgggattgac atcaagacgg
     1261 gctatctccg tttcgcaata tgcaaggcgg gaaaaggcgg caagagaaga agcaaacgcc
     1321 tcaacgtcaa gacgctccct tctccaccct gcgagcagaa gaggatagcc ctttgattcc
     1381 tttatcatcc tgagagcctc atctgtgtcg aagggagctg cccgcaggac tccgtccggg
     1441 aatatttctc cgaaaatacc gctgaaactc atctcgacaa ctggtccaaa acgcggatcc
     1501 ttccggtatt ccatacgcca ttccgttccc ccgtgcacca tttcctgaac aagaactccc
     1561 cgaatcctgg cagacggatt ggcaatgcgc gctttttcaa gcgttcgtcc gtaggcgttc
     1621 ttgagctctt cctcgttctg aacatccagg gcgataacgc gagcctccgt tttacgcaaa
     1681 atagatggag acatcacctt gagagcaata gggtagccta ttgtctccgc cgcggtcaaa
     1741 gcctcgggta gcgaatggca caaagcttct ctcgcaacag gtatatcgta cgctcgaaga
     1801 agcacttttg cgtcatattc agtcggacgg ggatgcagtg gacactttgt aggatgagcg
     1861 ccttcggacg gagccgggca caccgccggg ccgctcccga agaccagctg caaagcgtta
     1921 aagcaacggc gaataccggg gataagcggg attttcgccg aatacaactg ttcttcgaac
     1981 aatgcgttgt ccgccagaga tgaaaacaca ggaagccggc ctgactgttc gatacgttgc
     2041 tttcttcgct gaacggtatt cagagaatcg gatccagaaa ggacatcgat cagaaggggc
     2101 gacgtcgccc gagcgctctc ttgaagagta aattcagctc tttccgcttc tttttttatc
     2161 aacatggaaa gttcgtccga ggaggtatgg tatgcacatt ccttcccccc ggaaaggtca
     2221 ctgttcttca aagccagaaa ggctagggcc ccatcgacaa tctcctgaat atcgttcagg
     2281 agaaccacgc cgaattgctt cagggcagca gtttcctcac gggaactcgt ttggggccgt
     2341 cgaagaagaa gaacagcagt tctttttcct ttctttgcgg catccgatgc aaaatcaaga
     2401 aacttacgcc cttcacgtaa ttctttcaga ataaacacat agaaatcggt ttgagaatcg
     2461 gattgaagaa tcgaggaggc agcttcaagc ggagaatagt ccactgcctc gccgaaggag
     2521 aatgcgtacc tgagaggcgc tcttctttgt tgaaaagcgg agaggcagcg aacgagaacg
     2581 tcacccccat cagcaagcaa tgcggtgttt ccagggacgc ccggctgcat acagggtacg
     2641 ataattcctt cacttcgggg gccaagaata cgcattccat tttcacttgc tctggagcaa
     2701 agagagagag agagctttcg aggccaacgg ccgccaagca caaggaaggg aatgggaagt
     2761 tcaaaacatc tttcgagcat cttccggtct tcaggacctt ttcctgcgaa aaacagtgta
     2821 tccggaatgg gagaagacga agaaacgggg aacgagagtt cgggaaaagt gagctgcgga
     2881 gctaattgca gtactcgaaa actcgtttga tttcctccgc aaaaaaaagc attttgacct
     2941 ctcccgggca gtcgagctat tgtatccata acgctcttcc tttctggcaa gccaaaaaca
     3001 atggggagag cgcgccgcta ctccccatcc gaaaagcggg aaaaatatct aactccctag
     3061 agaattcctt tgatcctgtc ggaacctctc ttgatattga gctccacaag atccttgttc
     3121 tgcaaaacat cccactttag attcagcacc aacaggaaaa gggcagagta gccgtcaacc
     3181 ctccacgact gcgcttctct ttcgaatgct ttatacactg tagcaacttg ctctctcccc
     3241 ttttcttcct cttcgaggga ggcaatgaac cgctgaaatt catcgggaga atccatttcg
     3301 gaagcacgaa gaatatccca aagataagag agactggtct ccagatattc gggatcgggc
     3361 ctgaacggag ggaatccgac atcagaagct atctccgccc atcggcgggg aatctcttcg
     3421 tgttcttgga aatactgtct caatgcgttt ctgtcgaaaa cccgtcgggt tgatgcgaca
     3481 ggctcaaccc gggcaacggg aggagtgacg ggcattgcag attctcgggc agattcccaa
     3541 agagaaaaat acccttcgtc agcttcttcc agaagagcgc ctacccgaaa gagttttctt
     3601 tgaattttct tttttgtgct cgtgtcagtc ggaagcatga ccttcggtgt attcgcagcc
     3661 cagacctcct gaagaaccgt tcgtatctcc aaagaaaacg gaatgtcctt atacttgctc
     3721 cactctcgca ttgctgcccg tgcgcggctt agggagagcg tataatacac agcaggataa
     3781 ccgaaacgat cgggatcatc caagcttgcc gatgtaacgg accgtgccat atccacaagg
     3841 aattcttgtt gtacaattcg ctccactttt tctacatcct ctgggaaatg gacaagaatc
     3901 ttgacggtgc agagatcggc aatttctgaa agagatgagg gaactccctc ggcgagggcc
     3961 tcatacatcc gaaggaaacc ctccggggtt tttgcctctc cagacacgtt atacagttga
     4021 atgccatcct gctccacaag gttctccaat agattgcagg ccctcgcggc aaactcgagg
     4081 taaagagagt actgtcgcat gtacttcttt cccaattcgt caactttcgt tgcattcatc
     4141 gattaatcac cgcccataaa aataaagtgc tagagtctgc acgattttcc atttaccaca
     4201 gaagccagta cggggccgct tgcagcaagc cctcataggg cgagtacgga acagacagtc
     4261 cacaggcaag tatatagtaa atcagcttta ttgaaaagct tttcctcaat cctcgaattc
     4321 aagcgcgagc agatcgaaac cgcgtactac aactcgcaga a
//
LOCUS       DAOTIL010000115         4325 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminobacteriaceae bacterium isolate AMR_MDS_3026
            NODE_24127_length_4325_cov_3.782436, whole genome shotgun sequence.
ACCESSION   DAOTIL010000115 DAOTIL010000000
VERSION     DAOTIL010000115.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868050
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminobacteriaceae bacterium (wastewater metagenome)
  ORGANISM  Aminobacteriaceae bacterium
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae.
REFERENCE   1  (bases 1 to 4325)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4325)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:19:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,224
            CDSs (total)                      :: 3,198
            Genes (coding)                    :: 3,184
            CDSs (with protein)               :: 3,184
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4325
                     /organism="Aminobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_24127_length_4325_cov_3.782436"
                     /isolate="AMR_MDS_3026"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:3034609"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1258)
                     /locus_tag="P5201_03540"
     CDS             complement(<1..1258)
                     /locus_tag="P5201_03540"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRV97634.1"
                     /translation="MYKGTSGVCMKVMSVLILAVVVAALPALAQERQERTVETLMDYF
                     DSLVKQGMEATETLLKSKKREKSEVEAVWDDLNERFISVYSKVGHFEFDSMLKKERNE
                     ARASRASEWDNSMKLFIHDLQKVGEVVAWQRKVNRDIAQLEYDIEKAPKRVEAYSGKL
                     KEEGPRVEKLLHELKSGSLSSAELQQRLDEVKNLRAEAENVSDSLERTITRLGEDLLL
                     KPEMDKRIAQILENWSNMKARHPAVADDLTDMPANWIPLVKKHWKDLEETREKFEEEY
                     APILDGKLFSDVPFFQNRPYKDLAKPVIELEIQIKTAIVSAVQKEKSVEDLRKAVALD
                     EELTRKEIQRIQELNSMISPEKWYALLLEDARAAGGASRVRELQLVMARHPEGSEEYR
                     KASEEAHLIQDEQHPEQLAVKKKLREF"
     gene            complement(1487..2686)
                     /locus_tag="P5201_03545"
     CDS             complement(1487..2686)
                     /locus_tag="P5201_03545"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRV97635.1"
                     /translation="MNRFRGQSFRLGFEDNEALKRIRDLKEKYPEDPAVEDLYQRARM
                     ALMKSKGDFMEITPEMLSFRENEQRLKKIFGEIADKEWKEFTGEILASPAVLPKAFPA
                     PDRSQVSIEDIKGHYVFLDEFEYPANQFIDLGREFVSVGTGARGYYFVEISNRNWLGP
                     YEAVKRYRRLINRDFPEEGKWTVLGKIVGIEMVIPQAEKVKTVAPQWGWVVSPVALYI
                     PGVTFAFFDASIEEGGYFAGEERMEEIKDPLYTVRYVPPDATPERLVEIFAASVKEKN
                     FDLFLDCIDPNRKKTRTALSLIRYHWDLHLDRFARFYVHVTTDEAKIEVLKGFQSSAG
                     SVEDFFLDEEQQEKIKEISGELVEQATVVSKAWDERGRQYGSPKPHYLIRRDKGRWYI
                     DNYEQPF"
     gene            complement(3026..>4325)
                     /locus_tag="P5201_03550"
     CDS             complement(3026..>4325)
                     /locus_tag="P5201_03550"
                     /inference="COORDINATES: protein motif:HMM:NF012422.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HRV97636.1"
                     /translation="IFLAVHALTYLFAAFFAPGEKGERARCAAAACLMLSLPLLIGLA
                     QYLPASFSAVAALSGMIFSSVGAALLIGLWALFLSSLIPDDVALVFGLAPLVAAFVMR
                     FAALSHILLLILGAPLFSALLLLLPSSDAHDESPDAIQSSSFPFWRLALFLFCLYAAN
                     GFLLSLMPELFPSPFPRTDQSLDWIPTISALGVAVVFRFYPRAELRTFYKGAFPFIAA
                     SLFLLLFSPLKTLSRFFMETGLAFLDLYAWLLLIYFASRAGEKRNAVIHGGIFLIVLA
                     GATAHLHLLLRNGAGGAIHGASELLIVVIPGVCLLLIMLALWDGREIPLQWERQDTRS
                     FPERDSGEETNFDHEKMPHPHEESASEKEEEMLLRMKLMEFNLTRQETEIALLLLQGL
                     KDRNICSLLYISQNTLKYHLRNIYRKTGSANRLELKNNVD"
BASE COUNT          992 a         1230 c         1094 g         1009 t
ORIGIN      
        1 caaactcccg cagtttcttc ttgaccgcca gctgttcggg gtgttgttca tcttgaatca
       61 gatgagcttc ctcggaggct ttccggtact cctccgatcc ttcaggatgc ctcgccatga
      121 caagctgaag ttcacgcacc cggctcgctc ctccggcggc tctggcgtct tcgagcagga
      181 gcgcatacca cttctcaggc gagatcatgc tattcaactc ctgaatgcgc tgaatctcct
      241 tcctcgtgag ctcctcatca agtgcgaccg cctttcgcag atcttcgacg cttttttctt
      301 tctgcacagc ggagacgatc gccgtcttga tctgtatctc aagttcaatc acgggcttcg
      361 caagatcctt gtacggtctg ttttgaaaaa acggaacgtc cgagaagagt tttccatcca
      421 gaatcggggc gtactcctct tcaaacttct cgcgagtctc ctcaagatcc ttccagtgct
      481 ttttcaccag aggaatccag tttgcgggca tatcggtcag atcgtccgca acggccggat
      541 ggcgggcctt catattcgac cagttctcca atatctgcgc tatccgcttg tccatctccg
      601 gcttcagcag aagatcttca ccgaggcgtg tgattgtgcg ttcaaggctg tccgacacgt
      661 tttcggcctc cgctctcagg tttttcactt cgtcgaggcg ttgctgaagc tccgcgctcg
      721 aaagggaacc gcttttcaac tcatggagga gcttctcgac gcgcggcccc tcttccttca
      781 attttcccga gtacgcctcc acccttttcg gcgctttttc tatatcgtac tcaagttgag
      841 caatatcccg atttaccttt cgctgccatg ccacgacctc ccctaccttc tgaagatcgt
      901 ggatgaagag cttcatgctg ttgtcccact cggaagctcg cgacgccctc gcttcgtttc
      961 gttctttttt cagcatgctg tcgaattcga aatggccgac cttgctgtac acggagatga
     1021 agcgttcgtt gagatcgtcc cagactgctt cgacctctga tttttccctc ttcttcgatt
     1081 tcagaagcgt ttccgtcgcc tccatgccct gcttgacaag agaatcgaaa tagtccatca
     1141 gcgtctccac agtccgctcc tgccgttcct gggccagggc gggaagagcg gcaacgacga
     1201 ccgcgagaat cagaacgctc atcaccttca tgcacacgcc cgatgtgccc ttgtacatgc
     1261 gtcattcctc ctcttcggca aaaaaacccg ccgtcaagtc ttcgccagcg tgatctcctt
     1321 gagcgccggt ggaagaccaa gcgtcttgcg cctggcggta tgcttttcca agataacttt
     1381 cgctgttttt cgcgcatctt ccagtccttt tctccgcgat cgctatacaa agttcgcaga
     1441 gtacgcattg tttcttcata ccgagcttca ccttctctcc cgaatattag aagggctgct
     1501 cgtagttatc gatataccat cgccccttgt ccctgcggat aaggtagtgc ggcttcggag
     1561 agccgtactg ccgcccccgc tcgtcccacg ccttgctgac gaccgtagcc tgttccacca
     1621 gctcgccgga gatctccttg atcttctcct gctgctcctc gtcgaggaag aagtcctcca
     1681 cgcttccggc gctgctttga aatcctttca acacctcgat cttcgcctcg tcggtggtga
     1741 catggacata gaatctggca aaccgatcaa gatgaaggtc ccagtggtag cgaataagag
     1801 agagcgcagt gcgcgtcttt ttgcggtttg gatcaatgca gtcaaggaag agatcgaagt
     1861 ttttctcctt gaccgaggcc gcgaatatct cgaccaagcg ctcgggcgtc gcatcgggag
     1921 gaacatatcg gacggtgtac agcgggtcct tgatctcctc catacgctcc tctccggcaa
     1981 aataccctcc ctcttcgatg gaagcgtcaa agaaggcgaa tgtaacgccc ggtatgtaga
     2041 gagccacagg actcacaacc cacccccact gcggggcgac ggtcttcacc ttctccgcct
     2101 gcgggataac catttcaatg ccgacgatct tcccaagcac agtccacttc ccctcctcgg
     2161 gaaagtctct gttgatcagc ctgcgatagc gtttgaccgc ttcataggga ccgagccagt
     2221 tcctgtttga tatttccacg aaatagtatc ccctggctcc agttccaacg gagacaaact
     2281 cccttccgag gtcgatgaac tggtttgccg ggtactcgaa ctcatcgaga aaaacgtagt
     2341 gccccttgat atcctctatg gagacctgag acctgtcggg cgccggaaag gcctttggaa
     2401 gcacggcggg gctcgcgaga atctccccgg tgaactcctt ccactccttg tccgcgatct
     2461 ctccgaagat ctttttgagc ctctgttcgt tctccctgaa ggagagcatc tccggggtga
     2521 tctccataaa atcgcccttg ctcttcataa gcgccatgcg cgccctctga tagagatctt
     2581 cgacagcagg atcctccggg tacttctcct taagatcccg aatacgtttc agcgcttcgt
     2641 tgtcctcgaa tccgaggcgg aacgactgtc ctctgaaacg gttcaccctg tcttcgaatt
     2701 ttttaaggaa gaactcggcg cgagtcacct cgttttttgg agcgggcgtc gcggactccg
     2761 ctccggaggg cggcaataca agtaggagga ggaaaaggaa aagaatcgcc ggcacacact
     2821 tgaatcgaca atacattgtg catcatctcc tctcgtttcc acggttcgtc tatacaagcg
     2881 gattgtagca tcaaagagag aaagtcaccc ccccgccgcg aactaaaagg ggtgggattt
     2941 cccacccctt ttaggggtga tattccccac ctttttttct atcgcatggg gtgaccggca
     3001 tcacccttga tgattccctt tttcgttagt caacgttatt tttcagttcg agacgattag
     3061 cggaccctgt cttgcgatag atattgcgga ggtggtactt gagggtattc tgcgagatgt
     3121 aaagtaatga acagatattc ctgtctttga gcccctgaag aagaagaagg gcgatttcgg
     3181 tttcctgccg tgtaagattg aattccatca gcttcatgcg aagaagcatc tcctcctcct
     3241 tctccgatgc gctttcttca tgaggatgag gcattttttc atgatcgaaa tttgtttcct
     3301 ctccactatc ccgttccgga aaggagcgtg tatcctgacg ctcccactga agagggattt
     3361 cccttccatc ccagagcgca agcatgatga gaagaaggca aacaccgggg atgacaacga
     3421 tcaggagctc cgacgctcca tggattgcac caccggcgcc gttacggagc agcaaatgaa
     3481 gatgcgccgt agcgccggcc agtactataa ggaaaatgcc tccgtggata accgcgttcc
     3541 gtttctcccc tgccctggag gcaaaataga taagaagcag ccaagcgtac aggtcaagaa
     3601 acgcgagtcc cgtctccata aagaagcggg acagcgtctt caagggagag aagagcaaaa
     3661 ggaagagcga cgctgcgatg aacgggaacg cgcctttgta gaacgtacgc agttcggctc
     3721 tcgggtaaaa gcgaaaaacc acggcaaccc ccaaggcaga tatcgtcggt atccagtcta
     3781 aagactggtc tgtccgggga aaaggcgagg gaaagagttc cggcatcaaa ctgagaagga
     3841 aaccgttggc ggcataaaga cagaaaagga agagcgcaag ccgccagaaa gggaaagaag
     3901 aggactgtat ggcgtcagga gattcatcgt gggcatcgct gctcggaagt agcaggagca
     3961 atgccgagaa gagcggcgct cctagaatca gcagcaagat atgagaaagc gctgcaaaac
     4021 gcattacgaa tgcagcgaca agcggcgcca aaccgaagac gagtgcaaca tcgtcaggaa
     4081 tgagtgagct gaggaaaaga gcccacaatc cgataagaag ggctgctccg acggatgaga
     4141 aaatcattcc ggaaagcgct gcaacggcgg aaaacgaagc aggaagatat tgagcaaggc
     4201 cgatcaggag aggaagcgag agcataaggc acgcggcggc tgcacaccgg gctcgctccc
     4261 ccttctcacc gggcgcgaaa aaagcggcaa aaagataggt caaggcatgc acggcaagaa
     4321 aaatg
//
LOCUS       DAOTIL010000116         4318 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminobacteriaceae bacterium isolate AMR_MDS_3026
            NODE_24186_length_4318_cov_3.395262, whole genome shotgun sequence.
ACCESSION   DAOTIL010000116 DAOTIL010000000
VERSION     DAOTIL010000116.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868050
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminobacteriaceae bacterium (wastewater metagenome)
  ORGANISM  Aminobacteriaceae bacterium
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae.
REFERENCE   1  (bases 1 to 4318)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4318)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:19:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,224
            CDSs (total)                      :: 3,198
            Genes (coding)                    :: 3,184
            CDSs (with protein)               :: 3,184
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4318
                     /organism="Aminobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_24186_length_4318_cov_3.395262"
                     /isolate="AMR_MDS_3026"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:3034609"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..525)
                     /locus_tag="P5201_03555"
     CDS             complement(<1..525)
                     /locus_tag="P5201_03555"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HRV97637.1"
                     /translation="MESLHNRSKGAGEWILENIVTLIFIAFTLFGFMVSSGVSVGYFF
                     SELSSRIFRNSFLVLSLIIPVLAGLGLNFGIVIGAMSGQVAISIARYFEMGGISGLLF
                     CFLVAALVAALCGYFTGKLYNKTRGQEMIASLIVGFFANGIYQFLFLFVVGVIIAVPA
                     THPMIKPDGIGIRMT"
     gene            complement(525..1598)
                     /locus_tag="P5201_03560"
     CDS             complement(525..1598)
                     /locus_tag="P5201_03560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014163876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HRV97638.1"
                     /translation="MNGNGNGNNRLRCVGGVLGGIGLPTLIIGGFWVFTVIAGHFVGI
                     SMSTLVSDTIKRAGMNGILVLAMVPAIQSGTGPNFALPIGIVCGLLGLVTAIELNLMG
                     MAWFLTSCAIAIPLAAIVGYGYGKLMNAVKGSEMTIATYTGFSIVALMCLAWLMIPFK
                     NPKMGWFIGTGLRETIQLDAVGGAQILNNFLMFTIGEVIIPTGLLLTFAFFSFLVWLF
                     FRSKRGIAISAGGINPKFARATGLDIDGNRVFANVISTVLGAIGIIVYSQSYGYAQLY
                     TAPLMMAFPAVAAVLIGGATASRAKVSHVIIGVVLFQGLLTTALPVANEVFAGTDLSE
                     IMRMIIQNGIILYALTQVKRGVQ"
     assembly_gap    1716..1725
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<1726..3227)
                     /locus_tag="P5201_03565"
     CDS             complement(<1726..3227)
                     /locus_tag="P5201_03565"
                     /EC_number="7.5.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014163877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter ATP-binding protein"
                     /protein_id="HRV97639.1"
                     /translation="MSESLLQFKNVSKNYYGNSVLTGINFDVKEGEIHALIGENGAGK
                     STLMNILFGMPVITSTGGYEGDVLIDGKKISFKSPHEAMYAGIGMVHQEFMLIPGFSI
                     TENIKINREITTQNVVSRVLGKRLETLDMVTMNADARKALDKLDMGVDEFIMVAGLPV
                     GHMQFVEIAREIDKTGIKILVFDEPTAVLTESEAQNLLAAVKRLAAKGIGIIFISHRL
                     DEIVTVADRVTVLRDGELVATKEIKDTNAVEIAALMIGRKVAISRVQSETRKVSDEIM
                     MSIRDLHVGMPGEMVKGIDLEIKKGEIVGIGGLAGQGKLGIANGVMGTYPSRGEVLFN
                     GKRLNLDDPHATIASGIGFVSEDRKGVGLLLNESIELNIAFTAMQVGNKFLKKSGPFR
                     FQDDAAIRRHALKMIKDLDIRCESPLQHAGALSGGNQQKVCVARALTQDPIFLFVSEP
                     TRGIDIGAKKLILDLLLKLNEEFGMTIVMTSSELAELRSICDRIVIVCEG"
     gene            complement(3374..>4318)
                     /locus_tag="P5201_03570"
     CDS             complement(3374..>4318)
                     /locus_tag="P5201_03570"
                     /inference="COORDINATES: protein motif:HMM:NF024095.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3798 domain-containing protein"
                     /protein_id="HRV97640.1"
                     /translation="HVTWPDNFMAEQEQMVSVVAKLAHDPNVKAVIINQAVPGTNAAV
                     DKLLETRKDVFIVYCTPQENPPDVAARAQIIMMPDELAMGNTIPTQAKKLGAKVFVHY
                     SFPRHMSQVLLSSRREIMKKKCAELGLEFVDATAPDPTGDSGLPGAQQFILEDVPKMV
                     AKYGKDTAFFSTNCAMQIPLIKSVVDTGAIYPQPCCPSPYHGFPTALGIESDLSKQDN
                     IKHVIDETRRILKEKGMTGRLSTWPVPVAMMGTVAGTEYAFKVIDGEVPFDKLDVKVL
                     EQCMSDYAKVAVETNPYVDEAGKKYDNFLFVLMDFLTY"
BASE COUNT          949 a         1266 c         1133 g          960 t
ORIGIN      
        1 ggtcatgcgg atgccgatgc cgtccggttt gatcatcggg tgcgtcgcgg gaactgcgat
       61 gatgacgccg acgacgaaga ggaagagaaa ctggtagata ccgttcgcaa agaagccgac
      121 aatcagactg gcgatcatct cctgaccccg cgtcttgttg tacagctttc cagtgaagta
      181 cccgcagagc gcggcaacaa gcgccgccac caggaaacag aacagaagcc ccgaaatgcc
      241 gcccatctcg aagtagcgcg ctatggatat ggcaacctgc cccgacatcg ccccgatgac
      301 gatgccgaaa ttcagcccga ggcccgcaag tacaggaatg ataagcgaaa ggacgagaaa
      361 agaatttctg aagatcctgc tggaaagctc cgagaagaag tagccgacgg agacgccgct
      421 cgacaccatg aagccgaaaa gcgtgaatgc gataaaaatc agcgtgacga tgttctcaag
      481 aatccactcg cctgcaccct tggatctgtt gtgtagactt tccattattg tacacctctc
      541 ttcacctggg tcagggcgta gaggatgatt ccgttctgaa taatcattct cattatctcc
      601 gacaggtcgg taccggcaaa tacttcgttc gccacgggaa gcgccgtggt cagcaagccc
      661 tggaagagga caacgccgat aataacatgg gatactttgg cgcgcgacgc ggtggcccct
      721 ccgatgagga ctgcggcaac cgccgggaac gccatcatca acggcgccgt gtacagctgc
      781 gcgtatccgt agctctggga gtagacgatg atgccgatcg cgcccagaac tgtggagatc
      841 acgtttgcga agaccctgtt gccgtcgata tcaaggccgg tagcccgcgc gaacttcggg
      901 ttgattcctc ccgcggaaat cgcgattccc cgtttcgagc ggaagaagag ccatacgaga
      961 aatgaaaaaa acgcgaacgt cagaagaagc cccgtcggga tgatcacttc cccgatggta
     1021 aacatcagga aattattgag aatctgcgcg cccccgaccg cgtcgagctg aattgtctcc
     1081 ctgagtccgg tgccgatgaa ccagcccatt ttggggtttt taaacgggat catcagccac
     1141 gcgagacaca tcagcgcgac gatcgaaaac cccgtatagg tggcaatcgt catctcggat
     1201 cccttcaccg cgttcatcag cttgccatat ccgtagccga caatcgcggc gagcggaatc
     1261 gcgatggcgc acgacgtcag aaaccatgcc attcccatta agttcagctc gatagcggtg
     1321 acaagaccga gcagcccgca gacgatgcct atcggcagag cgaagttcgg ccccgttccc
     1381 gactggatcg cgggcaccat ggcaagaacg aggataccgt tcatccccgc tcgttttatc
     1441 gtatcgctta caagggtcga catcgatatg ccgacaaaat gcccggcaat caccgtaaag
     1501 acccagaatc ccccgataat cagcgttgga agaccaatac cgcccagcac tccgccgacg
     1561 cagcggagac gattattccc gttgccgttt ccgttcatct cagacatgcg atactctctc
     1621 cttcggtgaa tcttccgctt ttttgactct gctgccggac atcatcaggc cgaaatcagc
     1681 gtccgacgca tccggggaca gtacgccctc gacctnnnnn nnnnntttcc ttcgcagacg
     1741 atgacgatcc tgtcgcagat gctccgaagc tccgcgagct ccgacgaggt catgacaatc
     1801 gtcattccga actcctcgtt cagtttcaaa agcaggtcga gaatcagctt cttcgcacct
     1861 atgtcgatac ctcgtgtagg ctcggagacg aaaagaaaga tcggatcctg cgtcagcgcc
     1921 ctcgcgacgc agaccttctg ctggttgccg ccgctcaagg cgcccgcgtg ctgcagaggc
     1981 gattcgcaac gaatatcgag atcctttatc attttcagcg catggcgtcg aatcgccgcg
     2041 tcgtcctgaa aacggaacgg accgcttttt ttcaagaatt tgttcccgac ctgcatcgcg
     2101 gtgaaagcga tgttcagctc gatggattcg ttgagcagca gcccgacgcc ctttctgtcc
     2161 tcgctgacga aaccgatccc cgaggcgatc gtcgcgtgcg ggtcgtcgag attcagccgc
     2221 ttgccgttga acagcacttc gcccctcgac gggtaggttc ccatgacgcc gttcgcgatc
     2281 cccagtttcc cctggccggc gagtccgcct atccccacga tctcgccctt tttgatctcg
     2341 aggtcgattc ccttgaccat ctcgccgggc attccgacat gcagatcacg tatgctcatc
     2401 atgatttcgt ccgaaacttt gcgcgtctcg gactgcactc tgctgatcgc caccttgcgc
     2461 ccaatcatca gggcggctat ttccaccgcg ttcgtatcct tgatttcttt cgtggccaca
     2521 agttcaccgt cgcgcaggac cgtgaccctg tccgcgacgg taacgatctc gtcgagcctg
     2581 tggctgatga aaataatgcc gattcccttc gcggcaagcc gtttcacggc ggcaagcaga
     2641 ttctgcgcct cactctccgt aagcaccgcc gtgggctcgt cgaagacgag aatcttgatg
     2701 cctgtcttat ctatctcgcg ggctatctcg acgaactgca tgtgccccac gggaagcccc
     2761 gcgaccatga tgaattcgtc gacgcccatg tcgagcttat ccagcgcttt gcgggcatcg
     2821 gcgttcatag tgaccatgtc gagcgtttca aggcgtttgc cgagaactct ggagacgacg
     2881 ttctgagtgg ttatttccct gttgattttg atgttctccg tgattgaaaa ccccggaatc
     2941 agcataaact cctggtgaac catgccgatt cccgcgtaca tagcctcatg cggcgacttg
     3001 aacgaaatct tctttccgtc gatcagcaca tcgccctcgt agccgccggt gcttgtgatc
     3061 acgggcatcc cgaagaggat attcatcagc gtggacttcc cggcgccgtt ctcccctata
     3121 agcgcgtgta tctcgccctc tttgacatcg aagttgatgc cggtcaatac gctgtttccg
     3181 tagtagttct tggaaacatt tttgaactgg agaaggcttt cgctcaacgc attacccccc
     3241 gtatctcaaa aaatataaaa gctgcaataa aagagggacg aacggattcg acaccagcga
     3301 ttcgcttcgg tgaaggatcc attcgtcccc ggacgctaca tttgttacag cttcttttta
     3361 tgcaaccctt cgattagtag gtcaggaagt ccataaggac gaacaggaag ttatcgtatt
     3421 tctttcccgc ctcgtcgaca tacggattcg tttccaccgc gaccttcgca taatcggaca
     3481 tgcactgctc cagaactttg acatcgagct tatcgaacgg gacttcgccg tcgatgactt
     3541 tgaaagcgta ctcggtacct gcgaccgtac ccatcattgc aaccggcacg ggccacgtgg
     3601 agagtcttcc cgtcatccct ttctccttga ggattctgcg cgtctcgtcg ataacgtgct
     3661 tgatgttgtc ctgcttggac aaatcggact cgataccgag agccgtcggg aatccgtggt
     3721 acggagacgg gcagcagggc tgcggataga ttgcgcctgt gtcgacaacg gacttgatca
     3781 gaggaatctg cattgcgcag ttcgtggaga agaacgccgt gtccttgccg tacttcgcga
     3841 ccatcttcgg cacgtcctcg aggatgaact gctgcgcgcc gggaaggccg gagtcgcctg
     3901 tcgggtccgg agccgtcgcg tcgacgaatt caagtccgag ttcggcgcac ttctttttca
     3961 tgatttctct gcgcgaggaa agaagaacct gggacatgtg gcgcgggaaa gaatagtgga
     4021 caaaaacctt tgcgccaagc ttcttagcct gggtgggaat cgtgttaccc attgcgagtt
     4081 cgtcgggcat catgataatc tgcgccctcg cggccacatc ggggggattc tcctgcgggg
     4141 tgcagtacac gatgaagaca tcttttcttg tttcaagaag tttgtcaacc gccgcgttcg
     4201 ttcccggaac agcctggttg atgataaccg ccttcacatt cggatcatgc gccagcttgg
     4261 ccacgacgct gaccatctgc tcctgctccg ccatgaagtt gtcgggccac gtaacgtg
//
LOCUS       DAOTIL010000117         4315 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminobacteriaceae bacterium isolate AMR_MDS_3026
            NODE_24214_length_4315_cov_2.979108, whole genome shotgun sequence.
ACCESSION   DAOTIL010000117 DAOTIL010000000
VERSION     DAOTIL010000117.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868050
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminobacteriaceae bacterium (wastewater metagenome)
  ORGANISM  Aminobacteriaceae bacterium
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae.
REFERENCE   1  (bases 1 to 4315)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4315)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:19:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,224
            CDSs (total)                      :: 3,198
            Genes (coding)                    :: 3,184
            CDSs (with protein)               :: 3,184
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4315
                     /organism="Aminobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_24214_length_4315_cov_2.979108"
                     /isolate="AMR_MDS_3026"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:3034609"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..742
                     /gene="pstA"
                     /locus_tag="P5201_03575"
     CDS             <1..742
                     /gene="pstA"
                     /locus_tag="P5201_03575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008520184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="phosphate ABC transporter permease PstA"
                     /protein_id="HRV97641.1"
                     /translation="SVLSLEFLIEPPRDSMTRGGIMTPLVGTLQLVGVAMFFAIPVGV
                     ATGLYFAEYARDDMFTSILRLAIRSLAGVPSVVFGLFGLSLFVILLGFGPSLLAAALT
                     LACLTLPLMVTASEQAFLAVPKDYRDASYALGASKWQTIRKVVLPAAAPTVITGIILS
                     VGRVAGETAPIMFTGAAFFAPHIARSLFSEVMALPYHIYVLATAGTFIEETRPIQYGT
                     VLVLLLLVLGICSGGIYMRSRLRRLMRR"
     gene            759..1517
                     /gene="pstB"
                     /locus_tag="P5201_03580"
     CDS             759..1517
                     /gene="pstB"
                     /locus_tag="P5201_03580"
                     /EC_number="7.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012868848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter ATP-binding protein
                     PstB"
                     /protein_id="HRV97642.1"
                     /translation="MEKILVSSRGVNLYYDSARVLKEITFDVMEKQVTALIGPSGCGK
                     SSFLRCINRMNDFIPSARVEGEILVGGSNIYAGGVDVIALRRNVGMVFQKPNPFPMSI
                     YDNVAYGPRLHGVKQKDRLDETVKQSLVGAALWDEVKDKLKTPGTGLSGGQQQRLCIA
                     RAIATEPEILLMDEPTSALDPMATARIEELVRELKKRYTVVIVTHNMQQAARISDRTA
                     FFLMGDLVEYDATPKMFTSPEDKRTEDYITGRFG"
     gene            1546..2268
                     /gene="phoU"
                     /locus_tag="P5201_03585"
     CDS             1546..2268
                     /gene="phoU"
                     /locus_tag="P5201_03585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014805925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0051049 - regulation of transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate signaling complex protein PhoU"
                     /protein_id="HRV97643.1"
                     /translation="MEPVSTRKQLEADLTELKRMVLRLGKMAEESISKAIWALKNQDA
                     ELARMVLAKDDDIDDLAEALDNACMHFAARFQPLGEDLRAVTSIMHMAVDLERIGDYG
                     GNIAKVAAELADKELIKPLIDIPRMLDVLSAMMSKALAAFDTNNAEMAKEVFAMDDMM
                     DDLEKQIMRELLLLMMERPQRIEQATQLLNVARTLERGGDHVTNVAERVVYILTGKTV
                     KASAFRRPRPAALNGRKEHEER"
     gene            2274..2963
                     /locus_tag="P5201_03590"
     CDS             2274..2963
                     /locus_tag="P5201_03590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013049061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HRV97644.1"
                     /translation="MPEARILIVEDEDAIMEIVSQALRRHGYETASASDGDAALEKVF
                     SLRPDLVILDLMLPKMDGWEVCRRMRAEPETERIPILMLTARRDERDVVEGLELGADD
                     YMKKPFSLAELTARVKALLRRTGERRESRIVENGDLRIELDDETALLRGTPLDLSPTE
                     FRLLEALARKMGRTASREELLSKIWSFYGGDTRTVDVHISRLRKKLDDGKSPALAINS
                     LRGRGYRLVWE"
     gene            2969..>4315
                     /locus_tag="P5201_03595"
     CDS             2969..>4315
                     /locus_tag="P5201_03595"
                     /inference="COORDINATES: protein motif:HMM:NF020037.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase dimerization/phospho-acceptor
                     domain-containing protein"
                     /protein_id="HRV97645.1"
                     /translation="MFTLKKKIVAVISLAVMLAFGISWLLLYGVVRNGIVRQSTLELS
                     QQTALLAASLKDGGMERFTANVEKWEDILRGRVTLIGANGVVLADTERSPEAMENHLT
                     RPEVKGALASGEGSALRYSATTNMYYLYFARKVEAEGRVAVLRAAYPLEFLSEAFAET
                     RNKFIFYLFVAVVLIVAFGTWLARLFFRPLDRIVEFAGKIAQGEAVRFPIMAEPELQR
                     LSSALDGMSARLRGALAELRTEREDLSRIVTALPVGVVLLDEERRVRYLNDVGKRLLC
                     VKEEAPHGVPVERILPSGDMYPLVEAAMNGEEDCLTLDLPELDKRYLRICSRRTSSGV
                     LLVISDLTEEYRLEQSRRDFIADAGHELQTPLASIRLAAEFLMEGPKRSEEDEKCLTT
                     IIAQQERMTRLVDDLLLLSRMKSEPPLHAEEEVDLCGLLSAVVEENRSHPLAGHIRV"
BASE COUNT          769 a         1193 c         1564 g          789 t
ORIGIN      
        1 cagcgtgctc tcgctggaat ttctgattga gcctccccgc gacagtatga cgcgaggggg
       61 gatcatgacg cccctggtgg ggacgttgca gctggtgggc gtggcgatgt ttttcgcgat
      121 tcccgtgggg gtggcgaccg ggctgtattt cgcggagtac gctcgcgacg atatgttcac
      181 gtcgattctg cggctggcga ttcgctctct tgcgggcgtg ccgtcggtgg tgttcgggct
      241 cttcggcctg tcgctcttcg tgattctgct ggggttcggt ccgagcctgc tggccgcggc
      301 gctcaccctc gcctgtctga cgctgccgct gatggtgacg gcgtcggagc aggcgtttct
      361 ggcggtgccg aaggactacc gtgacgcctc gtacgccctc ggggcctcga agtggcagac
      421 catccgcaag gtggtgcttc ccgccgcggc gccgacggtg atcacgggga tcattctgag
      481 cgtcggcaga gtggcggggg agacggcgcc catcatgttc acgggggcgg ccttcttcgc
      541 gccgcacatc gcgcgcagcc tcttctccga ggtgatggcg ctgccgtacc acatctacgt
      601 ccttgccacg gcggggacgt tcatcgagga aacccggccg attcagtacg gtacagtgct
      661 cgtgctgctg ctgctggttc tgggcatctg ttccggaggc atctacatgc gttcccgtct
      721 ccgcaggctg atgcggcgat aggggtggag ggactataat ggaaaaaata ctggtgagtt
      781 cacgcggcgt gaatctgtat tatgattcgg cgcgagtgct gaaggaaatc acgtttgatg
      841 ttatggaaaa gcaggtcacg gcgctcatcg gcccgtccgg atgcggcaag agcagctttt
      901 tgcgctgcat caaccggatg aacgacttca tcccgagcgc gagggtggag ggagagattc
      961 tggtcggcgg ttccaacatc tacgcgggcg gcgtggacgt gatcgcgctc cgccggaacg
     1021 tggggatggt cttccagaag ccgaacccct tcccgatgtc gatctacgac aacgtggcgt
     1081 acgggcctcg cctgcacggc gtcaagcaga aggacaggct ggacgagacc gtaaagcaga
     1141 gccttgtggg cgcggcgctc tgggacgagg tgaaggacaa gctgaagact cccggcacgg
     1201 ggctctccgg cgggcagcag cagcgtttgt gcatcgcgcg ggcgatcgcg acggagccgg
     1261 agattctgct gatggacgaa ccgacgagcg cgctcgaccc gatggcgacg gctcgcatcg
     1321 aagagctggt ccgcgagctg aaaaagcggt acactgtggt gatcgtaacc cacaacatgc
     1381 agcaggccgc ccgcatctcg gaccgtaccg ccttcttcct gatgggcgac ctcgttgagt
     1441 acgacgcgac gcccaagatg ttcacgtcgc cggaggacaa gcggacggag gactacatca
     1501 cggggcggtt cggctaggaa ggccgggaga gaggagacga tgagcatgga gcctgtgagt
     1561 acacggaaac agcttgaggc ggatttgacg gagctgaagc ggatggtgct ccgcctcggc
     1621 aagatggccg aggagtccat cagcaaggcg atttgggcgc tgaagaatca ggatgcggag
     1681 cttgccagaa tggtgctggc gaaggacgac gacatcgacg acctcgccga agcgctggac
     1741 aacgcctgca tgcatttcgc cgcgcgcttc cagcctttgg gcgaggatct gcgggcggtg
     1801 acctccatca tgcacatggc ggtggacctg gagcgcatcg gcgattacgg cggcaacatc
     1861 gcgaaggtag cggcggagct tgcggacaag gagctgatca agcccctgat cgacattccg
     1921 cggatgctcg acgtcctttc cgcgatgatg tcgaaggctc tggcggcgtt cgacacgaac
     1981 aacgcggaga tggcgaagga ggtcttcgcc atggacgaca tgatggatga tctggagaaa
     2041 cagattatga gggaactgct gctcctgatg atggagcggc cgcagcgcat cgagcaggcg
     2101 acgcagctgc tcaacgtggc gcggacgctg gagaggggcg gcgatcacgt gacgaacgtg
     2161 gccgaacggg tggtctacat tctcaccggg aagaccgtga aggcttcggc gttccggcgt
     2221 ccgcgccctg cggctctgaa cggacgaaaa gaacacgagg agcggtgagg gacgtgccgg
     2281 aagcgcggat tctcattgtg gaggacgagg acgcgatcat ggagatcgtc tcccaggcgc
     2341 tgcggcggca cggctatgag acggcctcgg cctctgacgg cgacgcggcg ctggagaaag
     2401 tcttctctct ccgacccgac ctggtgatcc tcgacctgat gctgccgaag atggatggct
     2461 gggaggtctg ccgccggatg agagcggagc cggagacgga gcggattccc atcctgatgc
     2521 tcaccgcgcg ccgcgacgag cgggacgtgg tggaggggct ggagctgggg gccgacgact
     2581 acatgaaaaa gccgttttcg ctcgcggagc tgacggcgcg cgtgaaggcg ctgctgcggc
     2641 gcaccgggga gcggcgggag agccgtattg ttgaaaacgg cgatcttcgc atcgagctcg
     2701 acgacgagac tgcgctgctg cgcgggacgc cgctcgatct gagcccgacg gagttccggc
     2761 tcctggaagc gctggcgcgg aagatggggc ggactgcctc gcgcgaggag ctgctgtcga
     2821 agatatggag cttctacggc ggcgacacgc ggaccgtgga cgtgcatatc tctcgcctgc
     2881 ggaagaagct tgacgacgga aagtctccgg cgttggctat caactccctt cgagggagag
     2941 ggtatcgtct ggtgtgggag tgatgtccat gttcacgttg aagaaaaaga ttgtcgcggt
     3001 gatctcgctg gcggtgatgc tcgcgttcgg tatcagctgg ctgctgctgt acggggtggt
     3061 gcggaacggc atcgtccgac agagcacgct ggagctttcg cagcagacgg cgcttcttgc
     3121 ggcgtcgctg aaggacggcg gtatggagcg atttaccgcg aatgtggaaa aatgggagga
     3181 tattctccgc ggacgggtca cgctgatcgg cgccaacggc gtggtgcttg ccgacacgga
     3241 gcggagcccg gaggcgatgg aaaaccacct gaccaggccg gaggtgaagg gggcgctcgc
     3301 ttcgggcgag gggtccgcgc tgcgctacag cgcgacgacg aacatgtact acctctactt
     3361 cgcccgcaag gtggaggccg aggggcgcgt cgcggtactc agggcggcgt acccgctgga
     3421 gtttttgtcg gaggccttcg cggagaccag gaacaagttc atcttctatc ttttcgtcgc
     3481 cgtggtgctg atcgtcgcgt tcggcacgtg gctggcgagg ctctttttcc gtccgctcga
     3541 ccggatcgtg gagttcgcgg gcaagattgc gcagggggag gcggtccgct tcccgatcat
     3601 ggcggagccg gagctgcaac gtctttccag cgcgctcgac gggatgtccg cccggcttcg
     3661 tggggcgctg gccgagcttc gcacggaacg cgaggatctt tcgcgcatcg tgacggcgct
     3721 gcctgtgggc gtggttctgc ttgacgaaga gcggagggtc cggtacctga acgacgtggg
     3781 gaagcggctt ctctgcgtga aggaggaggc gccgcacggc gtccccgtgg agcggattct
     3841 tccttccggg gatatgtacc cccttgtgga ggcggccatg aacggagaag aggactgcct
     3901 gacgctggat cttccggagc tggacaagcg ctatcttcgt atctgctcgc gacggacgtc
     3961 gagcggggtg ctgctcgtga tctccgacct cacggaggag taccggctgg agcagtcgag
     4021 gcgcgacttc atcgccgacg cggggcatga gcttcagacg ccgctggcga gcatcaggct
     4081 ggccgcggag tttttgatgg aggggccgaa gcggagcgaa gaggacgaaa agtgtctgac
     4141 gaccatcatc gcgcagcagg agcggatgac ccggctcgtc gacgatctgc tgctgctttc
     4201 gcggatgaag tcggagccgc cgctgcacgc ggaggaggag gtcgacctgt gcggactgct
     4261 gtccgccgtg gtggaggaaa accggagcca tcctctcgcg gggcatatcc gggtg
//
LOCUS       DAOTIL010000118         4311 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminobacteriaceae bacterium isolate AMR_MDS_3026
            NODE_24240_length_4311_cov_3.605028, whole genome shotgun sequence.
ACCESSION   DAOTIL010000118 DAOTIL010000000
VERSION     DAOTIL010000118.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868050
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminobacteriaceae bacterium (wastewater metagenome)
  ORGANISM  Aminobacteriaceae bacterium
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae.
REFERENCE   1  (bases 1 to 4311)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4311)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:19:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,224
            CDSs (total)                      :: 3,198
            Genes (coding)                    :: 3,184
            CDSs (with protein)               :: 3,184
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4311
                     /organism="Aminobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_24240_length_4311_cov_3.605028"
                     /isolate="AMR_MDS_3026"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:3034609"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            74..712
                     /locus_tag="P5201_03600"
     CDS             74..712
                     /locus_tag="P5201_03600"
                     /inference="COORDINATES: protein motif:HMM:NF024863.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDSL-type esterase/lipase family protein"
                     /protein_id="HRV97646.1"
                     /translation="MPIQRRLLFFLLLLLAGAASYWFFGPSRVNSWRMRNSPPAAFGP
                     VVAFGDSLTAGRGAGGAENSYPAALSLLLGRPVENMGVDGDTVARASERLEKVLELSP
                     SIVVVLLGGNDMLRRMNLDESFALLETMIRRIQQEGSLVFVAGLEGLPLLAPVGKRYF
                     EAAKNTGSVLIPDVLKGILGKPELMADGIHPNAAGYRVMAERIAKAMAPYLR"
     gene            complement(853..1314)
                     /locus_tag="P5201_03605"
     CDS             complement(853..1314)
                     /locus_tag="P5201_03605"
                     /inference="COORDINATES: protein motif:HMM:NF037131.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AsnC family transcriptional regulator"
                     /protein_id="HRV97647.1"
                     /translation="MDELDLRLIAELQENLPLTVAPYRTLAERLGVSEETVIARLQAL
                     RASGKLKRIGAVLRHQNSGYAANAMVVFKAPPDEMERLGKLLAESPLVSHCYERAPCA
                     EWPYTLYAMMHGRNMETIEEFVSIFALEHGVERFDILRSEEELKKSSLSFF"
     gene            complement(1320..1793)
                     /locus_tag="P5201_03610"
     CDS             complement(1320..1793)
                     /locus_tag="P5201_03610"
                     /inference="COORDINATES: protein motif:HMM:NF037131.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRV97648.1"
                     /translation="MLQERNMDNADRKLINLLQEGLPLERDPYGVFAAKLGVSREEIV
                     ERTGALMREGIIRRLGGVFDSGAMGYSSMLVGAHVPEDIFGDAARFVNAHPGVTHNYR
                     RSGFLNMWFTLSTVCDEERDAFLEELRRRFALSQVFPFPRLRSFKLRVFFDMEGA"
     gene            complement(1751..2764)
                     /locus_tag="P5201_03615"
     CDS             complement(1751..2764)
                     /locus_tag="P5201_03615"
                     /inference="COORDINATES: protein motif:HMM:NF015983.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HRV97649.1"
                     /translation="MLISWNTTRRCNLFCRHCYRESGPGEDIQGELSTAEGIELIASI
                     AEAEFRLLIFSGGEPLLREDILELIAAARDRGLVPAMGTNGTLLTESVARELAAAGLR
                     GIAISVDSLDREYHDAFRGSHGAYDLTQAGIRNALAAGLRVQINLTLTEKNLEEFDKM
                     VAYYESTGVHAIHPFFLVPTGRARSMPEEELKSGAYFGMIRKILERQATTTLELKPTC
                     APQFMPMAKEMGIAQRFTRGCLAGVSYCCILPKGEVHVCPYLPVEAGNVRRTAFADIW
                     RESEVFAELRDFSRYEGRCGACEYVAICGGCRARAFSYTGNYMAEEPWCFRSGTWTTP
                     TGS"
     gene            complement(2758..3936)
                     /locus_tag="P5201_03620"
     CDS             complement(2758..3936)
                     /locus_tag="P5201_03620"
                     /inference="COORDINATES: protein motif:HMM:NF015983.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HRV97650.1"
                     /translation="MISVTKLLLGREYSGDRLRYRRGTNSAGAADGLGPVVVWNCTRA
                     CNLLCRHCYASATPYACDAELSTGEAKDFLDTLAAFNVPALLLSGGEPLLRRDLFELL
                     EYARTLGLRTALSTNGTLIDERTAARIMELGVSYAGISLDGSRSVNDAFRGVDGAYDR
                     TISGFRNCRKAGQKAGLRLSMTRSTLKELPEIFRLVEDEGIARVCLYHLVPSGRGAEL
                     TDEEPSPEEKRAALDFLAEKTLDFAARGVETEILTVDNHCDGIYLYLREKERGGSRAA
                     EMLELLTASGGNRSGVAIGAVDWEGDVHIDQFTQEIILGNVRERDFPEIWRGETDSFL
                     HRLRDRKRHLRGRCRTCGWLEQCNGNLRARALAAGDFWGPDPSCYLTGDEIASGGATC
                     "
BASE COUNT          839 a         1388 c         1257 g          827 t
ORIGIN      
        1 gcgactttcc cgaaacgcac cggatcatta ggaaaacaat ttttcgcatc caattcggag
       61 gtgagagagc acaatgccga ttcagagacg tcttctgttt tttcttctgc tcctgcttgc
      121 cggagctgcg tcttactggt tcttcggtcc ctcgcgggtc aactcgtgga ggatgaggaa
      181 ctcccctccg gcggcattcg gccccgtagt cgccttcggc gacagcctga cggcgggcag
      241 aggggcggga ggagcggaaa actcctaccc cgccgcgctc tccttgctgc tgggacgccc
      301 ggtggagaac atgggcgtcg acggggacac cgtcgcaaga gcttctgaac gcctggaaaa
      361 agtgctggaa ctttcaccgt cgatagtcgt cgtgctgctc ggcggaaacg acatgctgcg
      421 ccggatgaac ctcgacgaga gcttcgccct ccttgagacg atgatccgga ggatccagca
      481 ggaaggatcg cttgtcttcg tcgccggtct ggagggactg ccgctactcg cgccggtcgg
      541 caagcgctac ttcgaggcgg cgaagaatac gggttccgtg ctcatccccg acgtcctgaa
      601 gggtattctc gggaaaccgg agttaatggc ggacggcatt catcccaacg ccgcggggta
      661 ccgcgtcatg gccgagcgca tcgcgaaagc gatggctccg tatctccggt aaacgcgccg
      721 gagaacatac tcattgtcga cgacaggagg ctgtcatgca ggagatactg gaccgaatcg
      781 taagcgaatg caggcgaaaa tagaataagg ggtgtcgtgc caagtacatc ccggaaccgg
      841 taaagggtcc ttttagaaaa acgacaggct gctcttcttg agctcctcct cgctgcggag
      901 aatatcaaat cgctccaccc cgtgctccag agcgaagatg ctcacgaact cctcaatcgt
      961 ctccatattc ctgccgtgca tcatcgcgta gagcgtgtac ggccactccg cgcacggggc
     1021 gcgttcgtag cagtggctca cgagcgggct ctccgccagc agcttgccca gccgttccat
     1081 ttcgtccggg ggagccttga agacgaccat cgcgttcgcc gcgtaccccg agttctgatg
     1141 ccgcagcacc gcgccgatcc gcttcagctt ccccgacgcg cggagcgctt gcaaccgtgc
     1201 tatcaccgtc tcctcggaga cgccgagacg ctcggccagc gtccggtagg gggcgacggt
     1261 cagcgggagg ttctcctgaa gctccgcgat cagccgcagg tcaagctcgt ccatagccct
     1321 cacgctccct ccatgtcgaa gaagacgcgc agtttgaagc ttctcagcct gggaaaggga
     1381 aagacttgcg acagagcgaa acgccgccgc aactcctcca gaaatgcgtc ccgctcttcg
     1441 tcgcatacgg ttgagagcgt aaaccacatg ttgaggaagc cgctcctgcg gtagttgtgc
     1501 gtcacgccgg ggtgcgcgtt cacgaagcgc gcggcatctc cgaagatatc ctccgggacg
     1561 tgggcgccga cgagcatgct ggagtacccc attgcgccgg agtcaaagac gccccccagg
     1621 cgacggataa tgccttcacg catcagcgca ccggttcgtt cgacgatctc ctctctcgaa
     1681 acgccgagct tcgcggcaaa aacaccgtag gggtccctct ccagaggaag cccctcctgc
     1741 agcagattga tcagcttccg gtcggcgttg tccatgttcc gctcctgaag caccagggct
     1801 cctccgccat atagtttcca gtgtaggaga acgcgcgcgc tctgcacccg ccgcagatcg
     1861 caacatactc gcatgcgccg cagcgcccct cgtagcgcga gaagtcgcgc aactccgcaa
     1921 agacctcgct ctcgcgccaa atatcggcga aggccgttct tctgacgttt cctgcctcca
     1981 ccggcaggta tgggcagacg tggacctcgc ctttcggcag aatacaacag taggagactc
     2041 cggcgagaca gccgcgcgta aaacgctgcg cgattcccat ctccttcgcc atcggcatga
     2101 actgcggcgc gcaggtgggc ttcagttcaa gcgtcgtcgt cgcctgtcgt tccaggatct
     2161 tccgtatcat cccgaaatac gcgccggatt tcagttcctc ctccggcatg gaacgcgcgc
     2221 ggcctgtggg aacaaggaag aacgggtgaa tggcgtgcac gcccgtcgat tcgtaataag
     2281 caaccatctt gtcgaattct tccaggttct tttccgtgag agtcaagttg atctgcaccc
     2341 tcagccctgc ggcaagggcg tttcgaattc ctgcctgcgt caggtcataa gccccgtgag
     2401 agcccctgaa cgcgtcgtgg tactccctgt ccaggctatc cacactgatc gcgatccccc
     2461 tgagaccggc cgcggcaagc tcgcgcgcga ccgattccgt cagcagcgtc ccgttcgtcc
     2521 ccatcgccgg gacgaggccg cggtcgcggg ctgcggcgat cagttcgaga atatcctccc
     2581 gaagcagcgg ttcgcccccg ctgaagataa ggagacggaa ctccgcctcc gcgatagagg
     2641 cgatcagttc gattccctcc gcggtggaca actctccctg gatatcctct cccgggccgg
     2701 actctctgta gcagtgcctg cagaagaggt tgcagcggcg cgtcgtgttc caggagatca
     2761 gcacgtcgcc cctccggacg cgatctcgtc gccggtgagg taacaggacg gatcgggacc
     2821 ccaaaaatcc ccggcggcga gcgccctggc ccggagattc ccgttgcact gttccagcca
     2881 cccgcaggtg cggcaccggc cgcggagatg ccgcttccgg tcgcgcagac gatgcagaaa
     2941 tgaatccgtc tctccccgcc agatctcggg gaagtccctc tcgcggacgt tgccgaggat
     3001 gatctcctgc gtgaactggt ctatgtggac atccccctcc cagtcgaccg cgccgatcgc
     3061 gacgccggag cggttgcctc cgcttgctgt aagcagttcc agcatctccg cggcgcggct
     3121 ccctccccgc tctttctccc gaaggtagag atagattccg tcgcagtggt tgtccacggt
     3181 gagaatttcg gtctccacgc cccgagcggc gaagtcgagc gtcttttcgg cgaggaaatc
     3241 cagagcggct cgcttctcct ccggcgaagg ctcctcatcg gtcagttcgg cgccccgccc
     3301 cgaagggacg aggtgataca ggcagactcg ggctattcct tcgtcttcga cgagacgaaa
     3361 gatctcgggc agctccttca gcgtactccg ggtcatggag agacgcaatc cggccttctg
     3421 ccccgccttc cggcagttcc tgaatccgga gatcgtcctg tcgtatgctc cgtcgacccc
     3481 gcggaaagcg tcgtttacgg agcgcgatcc gtcgagactg ataccggcat agctcacgcc
     3541 gagttccatt atgcgcgccg cagtccgttc gtcgatgagc gtcccgttgg tcgagagcgc
     3601 ggtgcgcaac cccagcgtgc gcgcgtactc caaaagctcg aagaggtccc tccgcaacag
     3661 gggctctccg cccgagagca gcaaggcggg gacgttgaag gcggcgaggg tatccagaaa
     3721 atccttcgcc tcgcccgtcg aaagctcggc gtcgcaggca tacggcgtcg ccgaggcgta
     3781 gcagtggcga cagagaagat tacacgcgcg ggtgcagttc cagacgacaa ctggtcccaa
     3841 accgtcggca gcgcccgcgc tgttcgttcc gcggcggtac cgcagtcgat cgccggaata
     3901 ctcgcgtccg agcagcaatt ttgtcacact gatcatcgaa accctccttg aggaaagtat
     3961 tcggcgagct gtatcagcca acggcgctgt acggataaat tacccgatga gcagccattc
     4021 gatcatcggg acaaacatgt tccatcacga cgccacaagc attgtatttg tccctttccc
     4081 ggatgtaaag cgcgaagagc cgttcatacc actgaaacag gggcaaaaat cacacgctct
     4141 tttgatataa ttgccgcaaa ttccgatggc gaacctcggc cttaaagccg ttcatcgccg
     4201 tgaaaggtgg taccgaatgc gcgacacaac cgctctgccc ggcttgatca tccggggtgc
     4261 cgtggaacag gatgttccgc tcatcctctc gttcatccgg gaacttgccg t
//
LOCUS       DAOTIL010000119         4296 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminobacteriaceae bacterium isolate AMR_MDS_3026
            NODE_24340_length_4296_cov_4.482905, whole genome shotgun sequence.
ACCESSION   DAOTIL010000119 DAOTIL010000000
VERSION     DAOTIL010000119.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868050
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminobacteriaceae bacterium (wastewater metagenome)
  ORGANISM  Aminobacteriaceae bacterium
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae.
REFERENCE   1  (bases 1 to 4296)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4296)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:19:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,224
            CDSs (total)                      :: 3,198
            Genes (coding)                    :: 3,184
            CDSs (with protein)               :: 3,184
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4296
                     /organism="Aminobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_24340_length_4296_cov_4.482905"
                     /isolate="AMR_MDS_3026"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:3034609"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            284..964
                     /gene="rpe"
                     /locus_tag="P5201_03625"
     CDS             284..964
                     /gene="rpe"
                     /locus_tag="P5201_03625"
                     /EC_number="5.1.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013047797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004750 - D-ribulose-phosphate 3-epimerase
                     activity [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribulose-phosphate 3-epimerase"
                     /protein_id="HRV97651.1"
                     /translation="MNRNLFFAPSLLAADILNMERSISSLEGEHDWLHVDVMDGCFVP
                     NITFGPGFVSALRKRFPQETLDVHLMIEHPELLLDDFLAAGADYLTIHAEADHHIHRT
                     LSRIRERGGKAGISINPGTSEEILRPLLPFVDLVLVMSVNPGFGGQSFIGSVMEKVTM
                     LSRWRTAGHYSYLIEMDGGIGRQNAAMVALGGTDVLVMGSAVFGTPEPARTLREIRTL
                     VKEATDRA"
     gene            957..1898
                     /locus_tag="P5201_03630"
     CDS             957..1898
                     /locus_tag="P5201_03630"
                     /inference="COORDINATES: protein motif:HMM:NF024855.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4115 domain-containing protein"
                     /protein_id="HRV97652.1"
                     /translation="MPEERENMLQEAGREIQRKREQLGLSLEDVHEGTKIRINFLQGI
                     EQGDYSGFPGTVYVRGFIRTYLQFLGAEELWGDFLPMLSEGVEKKRPEEPVIGTCTPP
                     TRGFKPASKFWVFVLLLLIVLGSGWYVWYSWEQSGVSLFSLNNDRESVSEEERSENEG
                     NGRTSEQSLTLEPPAETSDPQSGEISGDSSMAIGAPDTHPTFVLPPTAAELVGVAETP
                     VATPTPVVAEKKEKELVITANADCWVRVRQGTKTVFERTMRSGDTADFKVTERLSVTF
                     GRGSAIKVTWNGKDLGAPGRGVERIFYDPDGTTGRFD"
     gene            1898..3190
                     /gene="rimO"
                     /locus_tag="P5201_03635"
     CDS             1898..3190
                     /gene="rimO"
                     /locus_tag="P5201_03635"
                     /EC_number="2.8.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014163001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008172 - S-methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016782 - transferase activity,
                     transferring sulphur-containing groups [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0018339 -
                     peptidyl-L-beta-methylthioaspartic acid biosynthetic
                     process from peptidyl-aspartic acid [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12 methylthiotransferase
                     RimO"
                     /protein_id="HRV97653.1"
                     /translation="MKVYLISLGCAKNSVDSEKLLGVLQAFGVPVVHDIAEADIAIVN
                     TCGFIQSAVEENVNVILDLESMKEKGLLKKIGVVGCLVNRYGDELKKELPTVDLWAKA
                     EEWGNVAAFFGGSMTFEGKRGILQETRPWSRYLKVGEGCDTFCSYCTIPSIRGKARSI
                     PLPELLQEAQSLVQNGAKELCLVGQDLTIYGRDLYGDDGLRTLLRALDAELPEEVWIR
                     MLYLHPSRIDESFIDMVAENPRILSYLDIPIQHIDDEILHRMNRPDKEGHIRRIFAYA
                     RSVDPLFALRTTIITGFPGETERQFNKVLNFLEEAEIDRVGAFMYSPEEGTPAAQMEG
                     QVDASVKNSRYDRLMTLQAEISLERQRLFLGRTLSILVDEISQDDGFAWGRSYRDAPE
                     VDGLVGVAEGAGLTEGAFVEVLITDAEEHDLFAEISGN"
     gene            3147..3638
                     /locus_tag="P5201_03640"
     CDS             3147..3638
                     /locus_tag="P5201_03640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008710855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylglycerophosphatase A"
                     /protein_id="HRV97654.1"
                     /translation="MLKNMISSRKYQGTKDIAWCISTLCGLGFFSSMPGTLGSVAAFL
                     FFVVIPVPWYLILATAILGVSVSQLYSKRIGVEDPKEVIIDEVVGVWIAMYGMPSGFF
                     LPALFLFRVVDILKPFPVNASEKLPGGWGIMADDIVGGILVNLLLTFIRWLYIGGGWS
                     HIL"
     gene            3676..>4296
                     /locus_tag="P5201_03645"
     CDS             3676..>4296
                     /locus_tag="P5201_03645"
                     /inference="COORDINATES: protein motif:HMM:NF013183.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence/damage-inducible protein A"
                     /protein_id="HRV97655.1"
                     /translation="MKQAVLIALGDELLSGARREGNCGWLANRLALAGWKVLRMEIIP
                     DGLGALEDTLALWAGKTDLIVISGGLGPTHDDCTRKALARFLNAPLQVNAEAYNRILA
                     RYPSEMRENLELCRISQGSVPEGAQAIHNPRGSALGISFLMEGTKICAFPGVPDEFKA
                     MAEQELACEMSVSDCRMRSLIVVGWPESLLKDRIAPVVDDPSLHISI"
BASE COUNT         1052 a          963 c         1176 g         1105 t
ORIGIN      
        1 ctgtagacag accgccggca gcagcgacac cgacgccttc tacccctccc gctgttcgtc
       61 caggacccgc ttcgacagga acggcgagaa tacgatatca ggtgcctcca ttgacgaagc
      121 cgctctctct taagatagag atggtcgatg caacggggac gaaagcgttg cacgggcgcg
      181 atgtaaaagg cggagaattc atttctctcg acgctccctt tgtaacagaa gcagttgtta
      241 ctgtgtatct tggcggcgaa tttgtctggc aggagcgata taaatgaaca ggaacctttt
      301 ctttgcacct tctctcctcg ctgcggatat cctcaatatg gagcgttcta tctcttcgtt
      361 ggaaggcgaa catgattggc tccacgtgga tgttatggac ggatgttttg taccgaatat
      421 aacctttggc ccgggatttg tttctgcgct gcggaagaga ttcccccagg agactctcga
      481 cgtccatctc atgatcgagc accctgaatt gcttcttgat gattttttgg cagcaggcgc
      541 cgattatctg accattcatg cagaagcgga tcatcatatt cacaggaccc tgtcgcggat
      601 tcgggaaagg gggggcaagg ccggtatttc aatcaacccg ggaacttcgg aggagattct
      661 ccgtccgttg cttccatttg ttgatcttgt tcttgtcatg tcggttaatc ctggttttgg
      721 gggacaatca tttatcggat ccgtcatgga aaaggtgacc atgctctcca gatggagaac
      781 cgcaggccac tattcctatc tcattgaaat ggacggtggt atcgggaggc agaatgcagc
      841 tatggtggct ctgggaggga ccgacgttct tgtaatggga agcgcggtct ttggtacccc
      901 tgaacctgcc cgaaccctgc gcgaaatacg aacacttgtt aaggaggcaa cagatcgtgc
      961 ctgaagaaag agaaaacatg ctccaagagg ctggaagaga aattcaacgc aagagagaac
     1021 aactgggact tagtctcgag gacgtccacg agggaacaaa aatacgaatt aactttttac
     1081 agggaatcga acaaggagac tactcgggtt ttcctggaac agtgtacgtt cgaggattta
     1141 tccggacata tctgcagttc cttggcgcgg aagaactctg gggcgatttt cttccgatgc
     1201 tttcagaggg agtcgaaaaa aagcgaccgg aagagcctgt tattggaaca tgtacccctc
     1261 cgacgagggg gtttaaacct gcttctaaat tttgggtttt cgttcttctt ttgctgatcg
     1321 ttttggggtc gggttggtat gtttggtatt cgtgggaaca gagcggcgtt tccctctttt
     1381 cactgaacaa tgatcgggaa tctgtgtccg aagaagaacg atctgaaaac gaagggaacg
     1441 gacgaacttc agaacagagc ctaacccttg aaccccctgc tgaaacgtct gatcctcaat
     1501 caggtgaaat cagtggagat tcatctatgg caatcggggc tcctgatacg catccgactt
     1561 ttgttctccc tcccacagct gcggagcttg taggcgttgc ggaaacacct gttgctacgc
     1621 cgacgcctgt agttgcagaa aagaaggaga aagagctggt tatcacggcc aacgccgatt
     1681 gctgggtacg tgttcgtcaa ggaaccaaaa cggtgtttga gcgtacgatg cgatccggag
     1741 atactgccga ttttaaggta actgaaagac tgagtgttac ttttggacgg ggaagcgcca
     1801 ttaaagtgac atggaacggc aaggatttag gggctcccgg acgaggagtc gaaagaatat
     1861 tttatgatcc ggacggaaca acgggaaggt tcgattagtg aaagtttatc taatcagttt
     1921 aggatgcgca aagaactccg tggacagcga aaagctcctc ggagttcttc aagctttcgg
     1981 agtgcctgtt gtgcacgata ttgccgaggc cgatatcgcc attgtgaaca catgcggctt
     2041 tattcagtct gccgtcgagg agaatgttaa tgttatcctc gatcttgagt caatgaaaga
     2101 aaaagggctt cttaaaaaaa tcggcgtagt cggttgcctt gtcaacagat atggcgacga
     2161 gttgaaaaaa gagcttccaa cggttgattt gtgggcaaaa gctgaagagt gggggaacgt
     2221 tgccgccttt ttcggaggaa gtatgacctt cgaggggaaa aggggtattc tacaagaaac
     2281 gcgcccttgg tcacgctatc taaaagtggg tgaagggtgc gacacttttt gttcttactg
     2341 cactatccct tcgattagag gaaaggctcg cagcattcct cttcctgaac ttctccaaga
     2401 agcgcaaagc cttgttcaga acggagcgaa ggaactatgt cttgtaggac aggatcttac
     2461 aatttacgga agagatcttt acggagatga tggacttcgt acgcttttgc gagctcttga
     2521 cgctgagctt ccggaagaag tttggattcg catgctgtat cttcatcctt caagaataga
     2581 cgaatcattc attgatatgg ttgccgagaa tccgagaatt ttgtcctatc tggacatacc
     2641 gatacaacac attgatgatg agattttgca cagaatgaac cgtccggaca aagagggaca
     2701 cattcgtcgt atatttgctt acgcgaggag cgtcgatccg ctctttgcgc ttcgtacaac
     2761 gattattacc ggtttcccgg gggaaaccga gcgacagttc aacaaagtgc tcaattttct
     2821 tgaagaggcc gaaatagatc gcgttggtgc atttatgtat tctcccgaag agggaacgcc
     2881 ggcggctcaa atggagggac aggtcgatgc gtcggtaaaa aacagccgct acgaccgttt
     2941 aatgacgcta caggctgaga tatccctgga gagacaacga ctttttttag gacgtacgct
     3001 ttcaattctg gtcgatgaaa tcagccagga cgacggattt gcatgggggc gttcctacag
     3061 agatgctccc gaggttgatg gtctcgtagg cgttgccgaa ggcgccgggt tgacagaggg
     3121 agcttttgtg gaggtgttga taacagatgc tgaagaacat gatctcttcg cggaaatatc
     3181 agggaactaa agatatcgca tggtgtatca gcactctttg cggattgggt tttttctctt
     3241 cgatgccggg aaccctcggg tcggtagcgg cgtttctttt ctttgttgtc attcctgttc
     3301 cgtggtacct gattcttgct acagccattt tgggagtctc tgtgtcgcag ctatattcca
     3361 aaagaatcgg cgtcgaagat ccgaaagaag taattattga cgaagttgtc ggcgtctgga
     3421 tagcaatgta tggaatgccc tccggttttt ttctcccagc gctctttctt ttccgggttg
     3481 tggatatatt gaagccgttt cctgtgaacg cctcggaaaa acttcccgga ggatggggaa
     3541 taatggcgga cgatatcgtc ggaggcattc tcgtcaatct actcctgacc tttatccgct
     3601 ggctctacat cggaggcggc tggagtcata tcctgtaaaa aggaacgacg gcttttcgga
     3661 aaaaggaggc ataccatgaa acaggcagtc ctcattgccc ttggtgatga acttctcagc
     3721 ggtgcacgtc gcgaggggaa ttgcggatgg ctcgcgaatc gtcttgctct tgcagggtgg
     3781 aaagttctcc ggatggaaat catacccgac ggactcggag cgctggagga cactctggct
     3841 ctttgggctg gaaaaacgga ccttattgtc atctcaggag ggctcgggcc aacacatgat
     3901 gattgcacaa ggaaggccct tgcacggttt ttgaacgccc cgcttcaggt gaacgcggaa
     3961 gcatataaca gaattttagc acgatatcct tccgagatga gagagaacct tgaattatgc
     4021 agaatttctc agggctcagt tcctgaaggc gcccaggcta tacataaccc gcgcggctcc
     4081 gctcttggta tatcctttct gatggaaggg acaaagatct gcgcctttcc gggtgttccc
     4141 gatgagttca aagcaatggc tgagcaggaa cttgcctgcg agatgtctgt aagtgactgc
     4201 aggatgcgtt cactcattgt tgtcggatgg ccggagagtc ttttgaaaga ccgaattgct
     4261 cctgtggttg acgatccctc cctccatatt tccatt
//
LOCUS       DAOTIL010000120         4285 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminobacteriaceae bacterium isolate AMR_MDS_3026
            NODE_24429_length_4285_cov_3.273522, whole genome shotgun sequence.
ACCESSION   DAOTIL010000120 DAOTIL010000000
VERSION     DAOTIL010000120.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868050
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminobacteriaceae bacterium (wastewater metagenome)
  ORGANISM  Aminobacteriaceae bacterium
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae.
REFERENCE   1  (bases 1 to 4285)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4285)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:19:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,224
            CDSs (total)                      :: 3,198
            Genes (coding)                    :: 3,184
            CDSs (with protein)               :: 3,184
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4285
                     /organism="Aminobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_24429_length_4285_cov_3.273522"
                     /isolate="AMR_MDS_3026"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:3034609"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            441..1808
                     /locus_tag="P5201_03650"
     CDS             441..1808
                     /locus_tag="P5201_03650"
                     /inference="COORDINATES: protein motif:HMM:NF024573.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HRV97656.1"
                     /translation="MKRRYMKQQWTLRFKKLGLIEEGAVRIAPLMIFTGDNNTGKSYV
                     MTLLWGVIALGRSLFPTSIPESDSYRQCEKWLGSFSQEGEDEKMLLPEDEEVFVRWFG
                     DVLNQRRQTLCDELFGENAVRLRELRITDFVRERPLSLRWNKDAAEGSRYSSGEHYVR
                     FPLPSSPPPRAEVYRMIQYLTWKLIMDDLSAPLYPPRSPVKTVPPGEILYLPAARTGF
                     VLLRKALVSAALSMGGESVPLLLPVRRFVQRLLTLSFKEKNRFSAFADILERRILRGH
                     IHPDGAPLPEYRYSPQGQDNEALPLWRTSSLVTELAPLLVFLRGNDDFRSLIIEEPEA
                     HLHLEIQRELARLLVRLVNSGLPVWITTHGDTFFQQVSNLVKASAMDDAALSRLDLEP
                     EESIQPERVSAWHFQRVSASSGRDRTEISPLHADHDGIAATAFNAAIAELTRQTLDLS
                     PDDEA"
     gene            1798..2334
                     /locus_tag="P5201_03655"
     CDS             1798..2334
                     /locus_tag="P5201_03655"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRV97657.1"
                     /translation="MKRERVESTAATEGTQLVVRESEESGEAELTLNIARNGVLFQYP
                     HNNNFIFSNKKRADGILFLQRENDWEVMIVELKKTVKAKEWMKIKEQWHGAWLHALAL
                     SGVLEAPFPNPPRFLVAYRKDGIGENAPDPILLKSPEYALAFIEWRKGRAALNEIGEA
                     AFEKAPLDETGRGELRIP"
     gene            complement(2436..2957)
                     /locus_tag="P5201_03660"
     CDS             complement(2436..2957)
                     /locus_tag="P5201_03660"
                     /inference="COORDINATES: protein motif:HMM:NF024087.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3795 domain-containing protein"
                     /protein_id="HRV97658.1"
                     /translation="MHEEMSCKEQDKTLAAVCGLYCEACSLFIATKEDPARLTALAKM
                     FQLPEEEMRCYGCRSEKRGPYCTTCRMFVCAAERGIDFCGDCDEYPCDMLKAFQAERP
                     HRAELWEDLERIRDVGYAQWMTEKREHYACPQCATLNSAYDLKCRKCGAEPSCAFTAR
                     HREVIEEAMKRAR"
     gene            complement(2987..3886)
                     /locus_tag="P5201_03665"
     CDS             complement(2987..3886)
                     /locus_tag="P5201_03665"
                     /inference="COORDINATES: protein motif:HMM:TIGR01250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="proline iminopeptidase-family hydrolase"
                     /protein_id="HRV97659.1"
                     /translation="MHRTPPAAEGFIQFKGYKIWYRVVGEREEPGKLPLLCLHGGPGA
                     PHDYLEPLEALASGGRRVFFYDQLGCGNSDRPSDPSMWTVGLFLEELGVVRKALGLDR
                     VHILGQSWGGMLAMEHALTRPSGLASMIVADSPASMLQWVSEANLLRAALPPETQRRL
                     LEHEAAGTTDTPEYQEAMNVFYKRHLCRLDPWPECLLRAFEKTGFEVYTSMNGPSEFH
                     VTGKLKEWDIVERLPEIDAAALVIGGRYDEATPAITETVHRGIRGSEWVIFEESAHMP
                     HLEETERYLRVVGDFLNRIEARL"
     gene            complement(3944..>4285)
                     /locus_tag="P5201_03670"
     CDS             complement(3944..>4285)
                     /locus_tag="P5201_03670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015555910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HRV97660.1"
                     /translation="GLSAMKGDPENLVRRSLRVLAPGGTAFFSSYSPKFWEHRLAWFQ
                     EQAGKGLLGEIDMENTGNGRIVCKDGFTATTFDREDMERLGKASGRPYRVEEVKDSSI
                     FLILRNEAPRS"
BASE COUNT          850 a         1268 c         1270 g          897 t
ORIGIN      
        1 tgcaggagga gggaacctgc tgggcggggg gaagcgtctg gaaggggatg gccgccatgc
       61 gcatctcggt ctccaactgg tccacgaccg aagaggacat ccgccgttcc gcagccgcca
      121 tacggagcgt cctggcggag gtttcgcagc tcgccgaaag cgagtgaacg ggaggcgcgg
      181 agaaagcgag ttcagttgtc gagggttcgg cgaagaggag caatgaaagc attcgataga
      241 acaagttcgg aatttaccgt agaattgaca cgccctcggt ggtttgggaa gagcgttgcg
      301 tagcccggcg acttctccaa acctcggggg cctttttatt gctcggaaaa tccatagccg
      361 acacgcggga tcgaagtcga tggtataata aatttcgatt ttagcatgct attctatgct
      421 ccctgaaaag agggtgttcc atgaaacgtc gatatatgaa gcagcagtgg accttgcgct
      481 tcaaaaaact tggactgata gaggaaggag ccgtccggat agctcctctc atgattttta
      541 ccggcgataa caacaccgga aaaagctacg tcatgactct gctctggggg gtgatcgcgc
      601 tcggacgcag cctcttcccg acgtcgattc cggagagcga ctcgtatcgc cagtgcgaaa
      661 agtggctggg gagtttttcg caggaaggtg aagacgaaaa aatgctgctc cccgaagacg
      721 aggaggtttt cgtacgttgg ttcggagacg tgctcaacca gcggcgacag actctctgcg
      781 acgagctttt cggggaaaac gcagtgcggc ttcgcgagct tcgcattacg gattttgttc
      841 gcgaacggcc tctttccctt cgatggaaca aagacgcggc cgagggaagc cggtattcca
      901 gcggagaaca ctatgtccgc ttcccgctgc cgtcgagtcc ccctccccga gccgaagtct
      961 acaggatgat ccagtattta acctggaagc tgatcatgga cgatttgagc gcgcctctct
     1021 atcctccgcg atctccggtg aaaaccgttc ccccgggcga gatcctctac ttgccggcgg
     1081 cacgaacagg gttcgtgctc ttgcgaaaag ctcttgtttc cgcagcgctg agcatgggag
     1141 gcgagagcgt gccgcttctg ctgcccgtca ggcgtttcgt tcaacgcctt cttacccttt
     1201 ccttcaaaga gaaaaacagg ttctccgcct tcgcggatat tctggagcgc cgaattctgc
     1261 gcggacacat tcacccggac ggcgctccgc taccggagta ccgatacagt cctcaaggcc
     1321 aggataacga agcgctccct ctttggcgga cgtcgtcgct tgttacggag cttgctccgc
     1381 ttcttgtctt tctgcgaggg aacgacgatt ttcgctcttt gatcatcgaa gaaccggaag
     1441 cgcaccttca tctggaaata cagagagagc tcgcccgcct tctggttcga ttggtgaatt
     1501 ccggtcttcc tgtctggatc acgacgcatg gagatacctt ctttcagcag gtgagcaacc
     1561 tggtcaaggc gtcggccatg gatgacgccg ctctctcccg gctcgatctg gagcccgaag
     1621 agagcataca gccggaacgg gtttctgcgt ggcatttcca gagagtttcc gcctcgtccg
     1681 gtcgcgaccg aaccgaaatt tcgccgttgc acgccgatca cgacggaata gccgcaaccg
     1741 ccttcaatgc tgccatagcc gaactcactc ggcaaactct tgacttgagc ccggacgatg
     1801 aagcgtgaac gagtggaaag caccgcagca acggagggaa cgcaactcgt cgttcgagag
     1861 tccgaagaga gcggcgaggc cgaactgacg ctgaatatcg cacggaacgg agtacttttt
     1921 cagtacccgc ataataacaa cttcatcttc tcgaataaaa agcgcgcgga cggcattctc
     1981 ttcttgcaga gagagaatga ctgggaggtc atgatcgtcg aactcaaaaa gaccgtgaag
     2041 gccaaggagt ggatgaagat caaagaacag tggcatggag catggcttca tgctctggcc
     2101 ctctccggtg ttctggaggc tccttttccc aaccctcccc gttttctggt ggcgtaccgc
     2161 aaggacggta tcggcgagaa cgctcccgat ccgatcctgt tgaaatcgcc agagtatgct
     2221 cttgccttca tcgagtggag gaaggggcgc gccgccttga acgagatcgg agaagcagcc
     2281 tttgaaaaag cgcctctcga tgaaacggga agaggcgaat tgcgtattcc atgaaagacg
     2341 ccggaatccg gagcgaggaa gattgaataa acgcagatcg cgctgcatat ttttagcggt
     2401 gcgatctgcg tttattgtcc ggatgggcga gacttctatc gcgcccgctt catggcctct
     2461 tcgataactt ccctgtgcct ggcggtaaag gcgcagctcg gctccgcgcc gcacttccgg
     2521 cacttcaggt cgtaggcgga gttgagggtt gcgcactgcg gacaagcgta gtgctcccgt
     2581 ttttccgtca tccactgcgc gtacccgacg tcgcggatcc gctcaaggtc ctcccagagt
     2641 tcggcgcggt gcggccgttc ggcctggaag gctttgagca tgtcgcaggg gtattcgtcg
     2701 cagtcgccgc agaagtcgat tccccgttcg gcggcgcata cgaacatccg gcacgttgtg
     2761 cagtaggggc ctcgcttctc cgagcgacag ccgtaacagc gcatctcctc ctccggaagc
     2821 tggaacattt tcgccaacgc ggtcagccgc gccgggtcct ccttcgtggc gataaaaagc
     2881 gagcacgcct cgcagtacag gccgcacacc gcggcgagcg tcttgtcctg ctctttgcag
     2941 ctcatttctt catgcatgat gttccctcct tgagaatgtt gcgcgactac aggcgcgcct
     3001 cgatcctgtt caaaaagtcg ccgaccacgc gcaggtagcg ctccgtctct tcaaggtgcg
     3061 gcatgtgcgc gctctcctcg aagatcaccc attcggagcc gcgtatgccg cgatgcaccg
     3121 tttcggtaat ggcgggggta gcctcgtcgt agcggccgcc gatgacgagc gcggccgcgt
     3181 ctatctccgg aaggcgctcc acgatgtccc actccttgag tttgccggtg acgtggaact
     3241 cgcttggccc gttcatggag gtgtagactt cgaagcctgt cttctcgaac gctctgagga
     3301 ggcactcggg ccacgggtcg aggcggcaga ggtggcgttt gtagaagacg ttcatcgcct
     3361 cctgatattc gggagtgtcc gtagtaccgg cggcttcgtg ctcaagaagc cgccgctgcg
     3421 tttcgggggg aagcgccgcc ctcaggaggt tcgcctcgga aacccactga agcatgctgg
     3481 cgggggagtc ggcgacgatc atgctcgcca gtccggacgg ccgcgtgagg gcgtgctcca
     3541 tcgcgagcat gcctccccag gactgtccca ggatgtggac gcgatccagt ccgagggctt
     3601 tccggacgac gccgagttct tcgagaaaca gaccgaccgt ccacatcgac gggtcgctcg
     3661 ggcggtccga attgccgcac cccagctgat cgtagaaaaa cactctccgt ccgcctgagg
     3721 ccagcgcctc cagcggttcg agatagtcgt gcggcgcgcc ggggccgccg tgcagacaga
     3781 gaagcggcag ttttcccggc tcttcccgct ccccgacgac gcggtaccag attttatacc
     3841 ccttgaactg gatgaatccc tctgcggccg gcggtgtccg gtgcattcgt accccctcct
     3901 atttcatgat gatcgctatc gctcgcatga ggcgccgcgc gcttcacgag cgcggagcct
     3961 cgttgcgcag tatcaggaag atgctcgagt ccttcacttc ctccacccga taggggcgtc
     4021 ccgaggcttt ccccaggcgt tccatatctt cgcggtcgaa cgtggtcgcg gtgaagccgt
     4081 ccttgcagac gatccggccg tttcctgtgt tctccatgtc gatctccccg agcaggccct
     4141 ttcccgcctg ctcctgaaac cacgccagac ggtgctccca gaacttcggg ctgtagctgc
     4201 tgaagaaggc cgtccctccg ggcgcgagca cgcgcaacga ccggcggacc aggttctccg
     4261 gatcgccctt cattgccgac agtcc
//
LOCUS       DAOTIL010000121         4282 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminobacteriaceae bacterium isolate AMR_MDS_3026
            NODE_24456_length_4282_cov_3.420629, whole genome shotgun sequence.
ACCESSION   DAOTIL010000121 DAOTIL010000000
VERSION     DAOTIL010000121.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868050
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminobacteriaceae bacterium (wastewater metagenome)
  ORGANISM  Aminobacteriaceae bacterium
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae.
REFERENCE   1  (bases 1 to 4282)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4282)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:19:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,224
            CDSs (total)                      :: 3,198
            Genes (coding)                    :: 3,184
            CDSs (with protein)               :: 3,184
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4282
                     /organism="Aminobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_24456_length_4282_cov_3.420629"
                     /isolate="AMR_MDS_3026"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:3034609"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..385)
                     /locus_tag="P5201_03675"
     CDS             complement(<1..385)
                     /locus_tag="P5201_03675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006300176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HRV97661.1"
                     /translation="MQHVSSLTLYDQGDHKFIMLGWEEQEEEVAVQTNQYVISSGGEV
                     VLLDPGGAHVFPRVLANVAEMFELKDIRHIFYSHQDPDVSSGITLWLSMAEKATVYIS
                     GLWVRFLPHFGIYDVKRIKAIPDKGD"
     gene            complement(462..2258)
                     /locus_tag="P5201_03680"
     CDS             complement(462..2258)
                     /locus_tag="P5201_03680"
                     /inference="COORDINATES: protein motif:HMM:NF012772.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HRV97662.1"
                     /translation="MDSRDDAAKTEEMKLEWEKMFFFCDDPARTRRYFQIIKAVFDNT
                     VEGIVITDANGIIRMVNAAFSTITGYAPEEALGKNPRILKSDRHPPEFYENFWKRLIN
                     DGKWEGEIWNRRKNGEVYPEWMVCSAVYDEEGNAEHYISVFNDLSEVKFKDAQIELRS
                     NYDYLTGLPNKEMFQSRLSGELAHRERNSERLALLVFDINRFKNINDTLGHFVGDSVL
                     RKVGSLLSECIGSGGLVSRIGGDDFHILLPDVHTPDEIVGQVKRVFECLSDPVDVEGH
                     RLFLSASVGISVFPEDGRDVDDLLKRADIAMGKAKESGISSYRFFTEELGRRASLKLT
                     LENALREGLERNEFHLHYQPKYCIRRGAFSGAEALLRWNSPEGPVPPSDFIPLAEETG
                     LILSLGEEVFRKVCRQIAEWRSRGYSPGEIAVNLSARQFHQKGLLSRMKGILGEYDVP
                     PSLLGIEITESGIMENLMDSIAVLSGMKDLGMTVYVDDFGTGYSSLNYLKRLPIDVLK
                     IDKSFIDGVLEDPNDAAITRAVVGIARSLGLRVVAEGVETPEQLDFLRRNGCDEIQGY
                     LLSRPLPPDAFEKFGESEIPLCFNGLSGYNHC"
     gene            complement(2266..3360)
                     /locus_tag="P5201_03685"
     CDS             complement(2266..3360)
                     /locus_tag="P5201_03685"
                     /inference="COORDINATES: protein motif:HMM:NF018613.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016758 - hexosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HRV97663.1"
                     /translation="MRPVHIVYVTAGNGHRMAARALREAFDREDVPNVVMDLLDFSSD
                     LFKWSYSDVYAFVSEHSHLACRIMYDLTNKNREESIVLRMYEKISLENVKKFMRYIAE
                     NEPEKCICTHFFPLNVLSRMKAAGLYGGDLYTVITDYGLHKMWVNEYVDRYFIPSEMV
                     REGLLESGVPKEKIIMSGIPVLPKFARISEGKRSFSGRRLPVLFIASSIYNTLVVGIL
                     KELERTGLPLDVTVVTGRNHDLADDLERFSFNSKLSLNVLGFVDTLDKLMTKHDVLIT
                     KPGGLTVSEALCASIPMILVNPIPKQETNNSAYLQSKGAGILARTPEDVGGLVRRFYD
                     DRSTLSSMQRAAASIAFPNAASAVVSVVLR"
     gene            complement(3563..>4282)
                     /locus_tag="P5201_03690"
     CDS             complement(3563..>4282)
                     /locus_tag="P5201_03690"
                     /inference="COORDINATES: protein motif:HMM:NF021387.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050043 - lactate racemase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lactate racemase domain-containing protein"
                     /protein_id="HRV97664.1"
                     /translation="GRKSILPGVAGRETVMNNHKMMTEPGVGIGVVDGNRLSEEMVEA
                     VEKFCPLHFILNCVSDSSKHIVRVVGGHWYDAWREGIATFRKYNFAPISRKADVVFLS
                     AGGYPKDINMYQAHKALYMASRAVRPGGTLVFFAELAEGYGHKVFEEWARRGMTHDEV
                     IAAFTADFRFGAHKLFYLAQLAKECTILLYSRAGEEDSRLMFCEKCDLTDTLPSLVRK
                     YGEDFTSYVIPQAGIVLPVEE"
BASE COUNT          929 a         1272 c         1168 g          913 t
ORIGIN      
        1 aatctccctt gtccggaatg gccttgatgc gttttacgtc gtagatgccg aagtgcggaa
       61 gaaagcgcac ccagagtccg gagatgtaga ccgtcgcctt ctcggccatc gacagccaga
      121 gcgtgatgcc cgaggagacg tcgggatcct gatgggagta gaagatatgc cggatatcct
      181 tcagttcgaa catctcggcc acattcgcca acaccctcgg gaagacgtgc gctccgcccg
      241 gatcgagcag caccacctcg ccgccggaag agatgacgta ctggttcgtc tggacggcga
      301 cctcttcttc ctgctcctcc cagccgagca tgatgaactt gtgatccccc tggtcgtaga
      361 gggtaagcga actcacgtgc tgcactccaa ccaactcctt ccgtatgcag aggagacgga
      421 cgtttctccg gggtatattc tattctacgg agcaccccgt ctcaacagtg attataacct
      481 gagagtccgt tgaaacaaag aggaatttca ctctccccga atttttcaaa ggcatccggc
      541 ggaagaggcc ggctcagcaa gtacccctgg atctcgtcgc atccgttgcg gcgcaaaaaa
      601 tcaagctgct ccggagtctc caccccttcc gctacaacgc gcagccccag cgaacgggct
      661 atgccgacga cggccctggt gatggcggcg tcgttgggat cctccagaac gccgtcgata
      721 aaactcttgt ctatcttcag tacgtcgatg ggaaggcgtt taagatagtt cagcgacgaa
      781 tacccggtgc cgaagtcgtc cacgtacacg gtcatcccca agtctttcat cccggaaagc
      841 acagcgatgg aatccatgag gttctccatg atgccggact cggtgatctc gatcccgagc
      901 agagagggcg gcacatcgta ctcgccaaga atacctttca tcctcgagag aagccctttc
      961 tgatgaaact ggcgggccga aagattcacg gcgatctcgc ccggagagta gccgcgactc
     1021 ctccattcgg ctatctggcg gcacaccttc ctgaagacct cttctcccag ggagaggatg
     1081 agccccgtct cctccgcaag cggaataaag tcggagggcg gtacggggcc ttccggactg
     1141 ttccagcgca ggagcgcttc cgccccggag aaagcccccc ggcgaatgca gtacttgggc
     1201 tgatagtgaa ggtgaaactc attcctttcg agcccctcgc gcagcgcgtt ctccagggta
     1261 agcttcagcg acgcccgtct tccaagctct tccgtgaaga atcggtagct gctgattccg
     1321 ctctcctttg ccttccccat cgcgatatcc gcccgtttga gcaggtcgtc gacatccctg
     1381 ccgtcctcgg gaaagacgct gatcccgacg ctcgcgctta aaaagagacg atgcccttca
     1441 acatccacgg ggtcgctgag acattcgaag acccgtttga cctggccgac aatttcatcc
     1501 ggggtatgga catccgggag cagaatatga aaatcgtccc cgccgatacg ggagactaat
     1561 ccgcctgaac cgatgcactc ggagagcaga ctgccgactt ttcgcagtac cgaatcgccg
     1621 acgaaatgac cgagcgtatc gttgatattc ttgaaacggt tgatgtcgaa gacaagcagc
     1681 gcgagccgct cgctgtttct ctcccggtgg gcgagttcgc cggagagacg gctctggaac
     1741 atctccttgt tcggcagacc ggtgagatag tcgtagttgg agcggagctc tatctgggcg
     1801 tccttgaatt tcacctcgga aaggtcgttg aatacggaga tatagtgctc cgcattcccc
     1861 tcctcgtcgt agacggcgga gcagaccatc cactcggggt agacctcccc gttctttctt
     1921 cggttccata tctccccctc ccacttcccg tcattgatga gccgcttcca gaagttttcg
     1981 taaaactccg gggggtgccg gtcggacttc aaaatccggg ggttcttgcc aagcgcctcc
     2041 tccggagcgt atccggtaat cgtcgaaaag gccgcgttga ccatccggat tatcccgttg
     2101 gcgtccgtga taacgatacc ttctacggta ttgtcgaaaa cagccttgat aatctggaaa
     2161 tagcggcgcg tccgggcagg atcgtcgcag aagaagaaca tcttctccca ctccagcttc
     2221 atttcttccg tttttgcggc atcgtctcgg gaatccatcg gccggtcacc tcaacacgac
     2281 cgaaacgacc gcggaagcgg cgttcgggaa ggcgatggag gctgcggcgc gctgcatcga
     2341 agagagtgtt gaacgatcgt cgtagaagcg acggacaagc ccgccgacat cctcgggagt
     2401 tcgggcgagg atccccgctc ctttcgattg aaggtacgcc gagttgtttg tctcctgctt
     2461 ggggatgggg ttcacaagga tcatcggtat ggaagcgcag agggcttcgc tcacggtgag
     2521 cccccccggt ttggtgatca gcacgtcgtg cttcgtcata agcttgtcca gcgtatcgac
     2581 gaatcccagg acattcaatg atagcttgga attgaatgaa aagcgttcga gatcgtccgc
     2641 gagatcgtgg ttccgccccg tcaccacggt gacgtcaaga gggagccccg tgcgctccag
     2701 ttctttaagt ataccgacga caagcgtgtt gtaaatgctg ctcgctatga agagcacagg
     2761 gagacgcctt ccggagaacg accgcttccc ctcgctgatt cgtgcaaact tggggagcac
     2821 cggtattccc gacatgatga tcttctcctt gggaaccccg gactcaagaa gcccctcccg
     2881 gaccatttcg ctcgggataa aatagcggtc cacatactcg ttgacccaca tcttatgcag
     2941 gccgtagtcg gtaataacgg tataaagatc cccgccgtaa agcccggccg ccttcatccg
     3001 ggagagaaca ttcaacggaa agaagtgcgt gcagatgcac ttctccggtt cgttctccgc
     3061 aatgtaccgc atgaatttct tcacgttctc cagagaaatc ttttcgtaca tgcggagaac
     3121 gatactctcc tcccggtttt tattcgtgag atcgtacatg atccggcagg caagatgcga
     3181 gtgttcgctg acaaaagcgt acacatcgct gtacgaccac ttgaagaggt cgctcgaaaa
     3241 atcgagaaga tccatgacga cgttcggcac gtcctcgcgg tcgaaagcct cccgaagagc
     3301 gcgggcagcc atacgatggc cgttccccgc cgtgacatac actatatgaa cgggacgcac
     3361 tgtttttccc tccgatgtct gagcattccc ctcgctgcgc acttcattca gcaacggaaa
     3421 ataccctgtg gattctcttt attgtaccgt atgaaatgag aaggaggaac aggaggaaaa
     3481 aagaaattcc gggttgaaaa gagagagggc cgccaagccg tacacttgaa ggccctctct
     3541 cttggaatta cgttgttgtg aactactcct cgacaggcag cacaatgccg gcctggggaa
     3601 tgacgtacga ggtaaagtcc tccccgtact tccgcacgag ggaaggaagc gtgtcggtca
     3661 gatcgcactt ctcgcagaac atgagacggc tgtcctcttc gccggctctg gagtacagaa
     3721 ggattgtaca ctcctttgcc agctgggcga ggtagaagag cttgtgcgcg ccgaagcgaa
     3781 aatccgcagt gaacgcggcg atgacctcgt catgcgtcat tccgcgccgt gcccactcct
     3841 cgaacacctt atgcccgtac ccctcggcaa gttcggcgaa gaagacgagc gtcccgccgg
     3901 gacgaactgc tctcgacgcc atatagagcg ccttgtgcgc ctggtacatg ttgatatcct
     3961 tcgggtaccc tcccgcggag aggaagacca cgtcggcctt cctggagatc ggggcgaagt
     4021 tgtacttccg gaaggtggcg atcccctccc gccacgcgtc gtaccagtgt cctccgacga
     4081 ccctgacgat gtgtttggag ctgtccgaga cgcagttcag gatgaagtgg agcggacaga
     4141 acttctccac cgcctccacc atctcctcgc tgaggcggtt gccgtccaca acgccgatac
     4201 cgacgcccgg ctcggtcatc atcttgtggt tgttcatcac cgtctcgcgc cctgcaactc
     4261 ccggaaggat gctcttccga cc
//
LOCUS       DAOTIL010000122         4267 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminobacteriaceae bacterium isolate AMR_MDS_3026
            NODE_24549_length_4267_cov_3.736230, whole genome shotgun sequence.
ACCESSION   DAOTIL010000122 DAOTIL010000000
VERSION     DAOTIL010000122.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868050
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminobacteriaceae bacterium (wastewater metagenome)
  ORGANISM  Aminobacteriaceae bacterium
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae.
REFERENCE   1  (bases 1 to 4267)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4267)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:19:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,224
            CDSs (total)                      :: 3,198
            Genes (coding)                    :: 3,184
            CDSs (with protein)               :: 3,184
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4267
                     /organism="Aminobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_24549_length_4267_cov_3.736230"
                     /isolate="AMR_MDS_3026"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:3034609"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(157..2766)
                     /locus_tag="P5201_03695"
     CDS             complement(157..2766)
                     /locus_tag="P5201_03695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014807244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HRV97665.1"
                     /translation="MKVITSHMGNDFDSLASMVAAQKLYPDAKLCLSGSAGRTVREFL
                     KKNASHWTVLTPRKIKFDEITMLIVVDSRSRSRIGPFASLLGKKSIAVHVYDHHPPSN
                     DDIDGEFLAIEPVGACTTLMVEILLEKRIPIAPHEATLFATGIYEDTGGLTFSGVTAR
                     DFAAVSRLKEIGGDIASIPSFIEMTFSAPERRILDALVENAWVRFINGAKAVFSAVSA
                     PSYVDGLSLFVHRLRDYFDADIAIAAVKMDTRTYLVGRSSEDALDMAAFLTPFGGGGH
                     PQAASVTLHNAKPLPLVREMEQLLHDAVKPSLTAGDIMTSPVMAVPPDSLVDDAYRTM
                     LRYGHSALPVVKGKNILGLITRKDLDKAHLHGFGKTLIREFMTEGVITVPCDASIREA
                     HRLLATHSIGRLPVVDGRRIMGIITRTDLVKALYPLSLPKEERETTPELPWMEDVTTL
                     LDEQLPPEQNKILALLGERAEKLGMRAYIVGGIVRDLLLRRENLDLDIVVEGDAIRFL
                     KSFEKDGFRVSVHERYKTGTLSFPRGVKVDVATARREFYEFPVAQPKVFSDSLKHDLY
                     RRDYTVNAMAISINQSTRGVLVDYFGGRMDLRRKKLKVLHNLSFVEDPTRVIRGVRLE
                     QRLGLTMEDNTLRLIRSCIRGGLLVRLSGFRLRSELELSFREKFPWAAARRMGELGVW
                     DVLFPGIRIDESVRRTFRRLGAFIARISRDFPDFKGRQWLAFFSALLMESSENIRISA
                     LDRLNLSESERGIVVKCLSGLGAAEHTLGGRSSPLNSEIAAFLDGHDPLEAFFWSAAT
                     ERWRVRRRILQYLTRLHRVRPILSGGDLLQLGYAATPRIGVILEKLRILRLDSVVQTR
                     EEEEEYVRKHFPL"
     gene            complement(2763..3626)
                     /gene="coaE"
                     /locus_tag="P5201_03700"
     CDS             complement(2763..3626)
                     /gene="coaE"
                     /locus_tag="P5201_03700"
                     /EC_number="2.7.1.24"
                     /inference="COORDINATES: protein motif:HMM:TIGR00152.1"
                     /note="Dephospho-CoA kinase (CoaE) performs the final step
                     in coenzyme A biosynthesis; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004140 - dephospho-CoA kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dephospho-CoA kinase"
                     /protein_id="HRV97666.1"
                     /translation="MLVSAVTGDVGAGKSTLTAFWREAGASVIDADEIVRMLWTRPEI
                     ITAAGFRWGEGIFDGKGAVLPSRVAARAFGDEKEYRWLCDLLHPLVRIEMERRTASLG
                     GWVVAEIPLLFESGPPWWVDVSVYVTAPDEFRTLRNSVRGWNEDEIPRRESFLLPSEE
                     KRRRASLVMENSGTLEEFRALAIKQADLFKRASAIVRGSVLFQKNEDARRYAEYLHKE
                     RLGTEFLCTPAETFDRNEKGWFFTFFTLEQWFSLLSQPHCMDSARGPYIERIRRIPWS
                     RSLTLLEELRP"
     gene            complement(3586..>4267)
                     /locus_tag="P5201_03705"
     CDS             complement(3586..>4267)
                     /locus_tag="P5201_03705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013048565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="3'-5' exonuclease"
                     /protein_id="HRV97667.1"
                     /translation="DSSVPLPPEEFWSSAASRKIIDGKAGVGFQQLAEHLARILHKSD
                     DLNDILVYILDTMGYESVLRNDDPEGWEERVENIRELRSIVPSGGDLAEMLAEAALFT
                     DLETLNLEDGNAVNLLTLHAAKGLEFPVVFLVGMEEAVFPHFKCLEDREGMEEERRLC
                     YVGMTRAEERLYLSGGRSRRLFGTTFRNGLSRFLWEIPDRFKRVDDRGEEEKTHVGFS
                     GNRRRWGW"
BASE COUNT          973 a         1229 c         1138 g          927 t
ORIGIN      
        1 aacactgaga agcagatctg caataccagg cattcgtaac aatagagtca ctctccttaa
       61 cttgttgtaa cgttttttgc cgcacctcgc cgttcagaac gtgctcctcc gtctcatcgg
      121 caaaccgtag ggaagagggg gcccgcgtgc tgcgcgttac agcgggaagt gttttctgac
      181 gtactcttcc tcttcttccc tggtttgcac tacgctgtcc aggcgcagga tccgcaattt
      241 ttcgaggatg acgccgatgc ggggggttgc ggcatacccc aactgcagaa gatcacctcc
      301 tgaaagaatc gggcggactc tgtgaaggcg cgtaaggtac tgaaggattc tcctgcggac
      361 tcgccagcgc tcagtcgctg cgctccaaaa gaacgcttct aatggatcgt gcccgtcaag
      421 aaatgcggca atctcggagt tgagcgggct gctcctacct cccagcgtgt gctcagcggc
      481 gcccaacccc gaaaggcatt ttactacaat tccgcgttcc gattcagaga gattcaaccg
      541 atccagcgcc gaaatacgta tattttcgga ggactccata aggagtgcgg aaaaaaacgc
      601 aagccattgg cgtcccttga aatccgggaa atccctggat attctggcaa tgaaggcgcc
      661 gagcctgcgg aaagtcctgc ggacgctctc gtcaattcga atacccggaa acagaacgtc
      721 ccaaaccccc agctccccca ttctccgcgc agcagcccag ggaaactttt cccggaatga
      781 gagctccagc tcgctccgga ggcgaaaccc tgaaagacgg accagaagtc cgcctcggat
      841 gcagcttcgt atcagccgaa gcgtattgtc ctccatggtc agtccgaggc gctgttccag
      901 ccgcacgccc cggataactc gcgtcggatc ttcaacgaag ctcaggttat ggagtacctt
      961 cagttttttt ctgcgaagat ccatgcggcc cccgaaataa tcaaccaaaa ctcctcgcgt
     1021 tgattgattg atggagatcg ccatggcatt caccgtataa tctctccggt acaggtcgtg
     1081 tttcagagag tcgctgaaga ctttgggctg ggctacagga aactcgtaga attctctgcg
     1141 tgcggtggca acatccacct tgacgccgcg gggaaatgaa agagtacccg ttttgtatcg
     1201 ttcgtgaacc gagactctga aaccgtcttt ttcaaagctc ttgaggaaac gtatcgcatc
     1261 tccttcgacg acgatatcaa gatcaaggtt ttctctccgc agaagaagat cgcggacaat
     1321 ccctccaacg atataggcac gcatacccag cttctccgcc cgctccccca gaagcgcaag
     1381 aatcttgttc tgctccggcg gaagctgttc gtccagaagc gtcgtgacgt cctccatcca
     1441 tgggagctcc ggagtcgtct cccgttcttc cttggggagg gagagggggt acagggcctt
     1501 gacgagatcc gtccgtgtga taatgcccat aatccgtcga ccgtccacta caggaagcct
     1561 gccgatacta tgcgtcgcca agagacgatg ggcctcgcgg atagaagcgt cgcacggaac
     1621 ggtgataact ccctccgtca tgaactcgcg aatcagcgtc ttgccgaaac cgtgaaggtg
     1681 cgccttatca agatccttac gggttatcaa acctaagata tttttcccct tgactacggg
     1741 gagcgcggaa tggccgtagc ggagcattgt cctgtaggcg tcgtccacaa gagaatccgg
     1801 aggcaccgcc ataaccggcg aggtcatgat gtctcctgcg gtgagcgatg gtttgacagc
     1861 gtcgtgcaaa agctgttcca tctctctgac aagcggcagc ggctttgcgt tatgcagcgt
     1921 cacggatgca gcctgaggat gtcctcctcc cccgaaaggc gtgagaaaag ccgccatgtc
     1981 gagagcatcc tccgagcttc gtccgacaag gtatgtcctg gtatccatct tgaccgcggc
     2041 aatggcaatg tcggcgtcga agtagtccct gagacggtgg acgaagagcg agagtccgtc
     2101 cacgtacgat ggagcggaaa cggctgagaa aacagccttc gctccgttaa tgaagcgtac
     2161 ccaggcattt tcaacaaggg cgtcgagaat gcgccgttcc ggggcggaaa acgtcatttc
     2221 tatgaaggag ggaatggaag cgatgtcgcc tcctatctcc ttcaggcggg aaacggcggc
     2281 aaaatccctc gctgtgacgc cgctgaaggt caatccgccc gtatcctcgt agatccccgt
     2341 agcaaagagc gtggcttcat gaggagcgat cggaatgcgt ttttccaaaa gaatctccac
     2401 catgagcgtt gtgcacgcgc cgacgggttc aatggcgaga aattcgccgt cgatgtcgtc
     2461 gtttgatggg gggtgatggt catagacgtg gactgcaata ctttttttcc cgaggagcga
     2521 ggcaaaagga ccgattcggg aacgtgacct ggagtccacg acaatgagca tggtgatttc
     2581 gtcaaacttg atctttctgg gcgtcagcac ggtccaatgt gaagcgttct ttttcagaaa
     2641 ctcccgcaca gtccggcccg cggagcctga aagacacagc tttgcgtcag gatagagctt
     2701 ctgcgccgcc accatgctcg cgagggagtc gaaatcgttg cccatgtggc tcgttatgac
     2761 cttcacggac gaagttcctc caataatgta agacttcggg accatggtat acggcgtatc
     2821 cgttcgatgt acggtccccg ggctgaatcc atgcagtgcg gctgcgaaag aagcgaaaac
     2881 cactgttcaa gcgtaaagaa ggtaaaaaac cacccttttt cattccggtc gaatgtctcg
     2941 gcgggagtgc aaagaaactc ggttcccaga cgttccttgt gaagatattc tgcatacctt
     3001 cgggcgtctt cgtttttttg aaagagaact gagcctctca ctatcgcgct cgctcgttta
     3061 aacaaatcag cctgtttgat cgcaagagcg cgaaactcct cgagcgttcc agagttctcc
     3121 atgaccagag aagcgcgtct ccgtttttcc tccgaaggca gaagaaaaga ctctcgccgc
     3181 gggatctcgt cttcgttcca tccgcgcaca ctgttccgta acgtgcggaa ttcatccgga
     3241 gccgttacgt agacgcttac atcgacccac catgggggcc cgctttcaaa aagaagagga
     3301 atctccgcca cgacccatcc gcccaaagac gccgttctgc gctccatctc gatccttacg
     3361 aggggatgca gaagatcgca gagccagcgg tactcttttt catcgccgaa tgctctggcg
     3421 gcgactcggg aagggagaac ggctcccttc ccgtcgaaaa tgccttctcc ccaccggaag
     3481 ccggctgctg tgatgatttc cggacgtgtc cagagcatgc ggacgatctc gtccgcatcg
     3541 atcacagacg ctcccgcttc cctccagaaa gcagtcagcg tcgatttacc agccccaacg
     3601 tctcctgtta ccgctgaaac caacatgtgt tttctcctcc tcgcctctat cgtccactct
     3661 tttaaagcgg tcggggattt cccagagaaa acgcgagagt ccgttacgga acgtcgtgcc
     3721 gaaaagacgg cgcgagcggc ccccggagag ataaagacgc tcttccgcgc gggtcatccc
     3781 cacgtagcag agccggcgtt cctcctccat gccttcgcgg tcttcaagac atttgaagtg
     3841 aggaaataca gcttcttcca tcccgacgag aaacaccacg gggaactcca gccccttggc
     3901 cgcatggagc gtcaggagat tgacggcgtt gccgtcttcc aaattgagcg tttcaagatc
     3961 tgtgaagagc gccgcttcgg cgagcatttc cgcgagatcc cctcctgacg gaacgatgga
     4021 ccgaagttcc cggatatttt cgactcgctc ctcccaccct tcggggtcgt catttcggag
     4081 cactgattcg taccccatcg tgtccaggat atacacaaga atatcgttca gatcgtccga
     4141 tttgtgaaga atacgcgcaa gatgctccgc cagctgctgg aaaccgacac ccgcttttcc
     4201 gtcgattatc ttgcgggaag cagcggagct ccaaaactcc tcgggcggga gaggaacgga
     4261 cgaatca
//
LOCUS       DAOTIL010000123         4264 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminobacteriaceae bacterium isolate AMR_MDS_3026
            NODE_24570_length_4264_cov_4.031599, whole genome shotgun sequence.
ACCESSION   DAOTIL010000123 DAOTIL010000000
VERSION     DAOTIL010000123.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868050
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminobacteriaceae bacterium (wastewater metagenome)
  ORGANISM  Aminobacteriaceae bacterium
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae.
REFERENCE   1  (bases 1 to 4264)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4264)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:19:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,224
            CDSs (total)                      :: 3,198
            Genes (coding)                    :: 3,184
            CDSs (with protein)               :: 3,184
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4264
                     /organism="Aminobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_24570_length_4264_cov_4.031599"
                     /isolate="AMR_MDS_3026"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:3034609"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            241..1509
                     /gene="tilS"
                     /locus_tag="P5201_03710"
     CDS             241..1509
                     /gene="tilS"
                     /locus_tag="P5201_03710"
                     /EC_number="6.3.4.19"
                     /inference="COORDINATES: protein motif:HMM:TIGR02432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016879 - ligase activity, forming
                     carbon-nitrogen bonds [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA lysidine(34) synthetase TilS"
                     /protein_id="HRV97668.1"
                     /translation="MLLAVSGGSDSMALMWFFHTLWEGHCIVAHLNHGIRGESADKDQ
                     RFVEEIAARFSFPVESASYPVPELLERGESLEDGARRIRYEFLEKTMKQTGACGVAVA
                     HTSDDSAETFLMNLMRGTGVRGLVGVPELRDSFIRPLLAFSKNYLKEILEFHGIPWRE
                     DLSNRDNAYTRNRVRNSLIPFLQKEFNPRVKEGILGIAEDMKHFRENEEEMQSALVDL
                     LERRVPFTEYACSLQAMRSLDEQTCAVFVRGVGARLGLRTLSRDRTNTLCRLVHKSSR
                     WCFQWQKEIVLFCSFHLVAWIDPAILVQSAGASPSSFALEEQSGFFEWGKWLFSWKRE
                     WASASNYGTMGAVFPNSERIEVVPFALFRKAIRKRDIPEWAVSLFPVVYSDRMQWLPY
                     WQGSGRWESLHDVAIRISAKYLQNIDERKR"
     gene            1520..2053
                     /gene="hpt"
                     /locus_tag="P5201_03715"
     CDS             1520..2053
                     /gene="hpt"
                     /locus_tag="P5201_03715"
                     /EC_number="2.4.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006583582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006166 - purine ribonucleoside salvage
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hypoxanthine phosphoribosyltransferase"
                     /protein_id="HRV97669.1"
                     /translation="MEYQISAILLSQEELAARVREIGETIGRDYAGKELVVVGILKGA
                     VIFLSDLVRAVPADVNITLDFMAVSSYGDSTKTSGIVRIVKDLDGSIKDKDVLLVEDI
                     VDTGLTLSYLVKMMRERQPKSVRVCALLDKEERRKVHVDVDYRGFPIPDEFVVGYGLD
                     YGGRWRHLPAIYTIEQK"
     gene            2094..4019
                     /gene="ftsH"
                     /locus_tag="P5201_03720"
     CDS             2094..4019
                     /gene="ftsH"
                     /locus_tag="P5201_03720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006301136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent zinc metalloprotease FtsH"
                     /protein_id="HRV97670.1"
                     /translation="MGRLVKNLGLYLILIVLVVSLVNVFLSPTQGPQQVQEIGYSTFL
                     SEVTAGRVSNVLVRENSIKGRFSDGKEFVSYVVGIGDLAKEVAQKGVAVEVEPPQRTP
                     WWANMLSSLFPTLLLIGVWIFFLYNMQGGGGKVMNFAKSKAKLFIDNRPKVTFKDVAG
                     CDESKEELSEVVYYLRDPSRFTALGARVPRGVLLLGPPGTGKTLLARACAGEADVPFF
                     SVSGSDFVEMFVGVGAARVRDLFEQARKYQPCIIFIDEMDAVGRHRGAGLGGGHDERE
                     QTLNQLLVELDGFDETTGIILIAATNRPDILDPALLRPGRFDRHIVVDRPDVKGREAI
                     LDVHVREKKLANDVDLAVLARRTPGFVGADLANLVNEAALLAARTGEKEIKMKDFEEG
                     IDRVIAGPERKSRLVSDKEKLIIAYHETGHALVAKRIPSCDPVHKISIIPRGHMALGY
                     TLQLPEEDRFLMSKNELLDKICVLFGGRVAEELRFGDVTTGASNDLERATQIARQMVT
                     EFGMSEKLGLVKLGHKHNEVFLGRDIGEERNYSDAIAYAIDQEVKAIIDSCYEKVRNI
                     LVENSEEMEKIAKTLLEKEIIEGKELNALLGLENPEEEKVVGPEEEERTETQETIAEE
                     NPVGESKNSDAATNAAI"
BASE COUNT         1081 a         1043 c         1144 g          996 t
ORIGIN      
        1 gagcatgcgg ttcatacccg cagtgtcact ggttcgattc cagtcaccgc caccacaaaa
       61 gacaaaagta aaaggcggag aaaatctccg cctttttttc cttttcagga ggtcgccgtg
      121 acttcaaaag agaaggtttc tcaattaaaa accatccctt tttctcattc tcaggagcgt
      181 ttacgcacaa ttttccatga tatcggcatc cgccaggggt ggtggcgcac tgaccgcccg
      241 atgttgctcg ccgtctccgg cgggagcgac tccatggcgc tcatgtggtt tttccatacc
      301 ctgtgggaag gacattgtat cgttgcgcat ctcaatcacg gcatcagagg tgaaagcgcg
      361 gacaaggatc agcgcttcgt cgaggagatt gccgcccgtt tttcatttcc cgtggaaagc
      421 gcttcgtacc ccgtacctga actccttgaa cggggggaat cgcttgaaga cggcgcacgg
      481 cgtattcgtt acgaattcct cgaaaagaca atgaagcaaa caggcgcttg tggagtagcc
      541 gtcgcgcata cttcagatga ttccgcggag acgtttttaa tgaatctgat gaggggaaca
      601 ggagtccgag gattggtcgg cgtccctgaa ctaagagact cctttatcag acctctcctc
      661 gccttttcga agaattatct caaggaaatt ctcgagtttc acggaatccc gtggcgggag
      721 gatctttcaa atagagacaa tgcatacacc cgaaaccggg tacgtaattc tctgattcca
      781 tttctacaaa aagaattcaa cccaagggta aaagaaggaa tccttggtat agcagaggac
      841 atgaaacatt tccgggagaa cgaagaagaa atgcaatccg cactcgtcga tctcctggaa
      901 agacgcgttc cctttactga gtacgcctgt tctttacagg caatgcgctc gctcgacgag
      961 cagacgtgtg cggtgttcgt cagaggggtt ggagccaggc tcggacttcg aacgctttcc
     1021 cgtgacagga caaacaccct ctgccgactt gttcataaat cttcgcggtg gtgtttccag
     1081 tggcaaaaag agatcgttct tttctgcagt tttcaccttg tcgcatggat tgaccctgct
     1141 atactagttc agtctgccgg cgcctcccca tcctcttttg ctcttgaaga acaatccggt
     1201 ttctttgaat ggggaaagtg gcttttttct tggaagaggg aatgggcatc cgcaagtaat
     1261 tacggtacaa tgggagccgt atttccaaac agcgaaagga ttgaggttgt cccttttgcg
     1321 ctgttcagaa aagcaatccg gaagagagat attcccgaat gggccgtgtc tctctttccg
     1381 gtggtgtatt ctgatcgaat gcagtggctt ccctactggc agggaagcgg tcgctgggaa
     1441 agtctgcacg acgttgcgat tcgtatttct gcaaaatacc tgcagaacat tgatgaaagg
     1501 aaaaggtgac gtaacgacaa tggagtatca aatttctgcg attctccttt cacaggaaga
     1561 attggctgcc cgcgtccggg agataggaga gacgatcgga cgcgattatg ccggtaaaga
     1621 attggtagtc gtaggcatcc tcaagggagc tgttattttt ctttccgact tggtgagggc
     1681 agttcccgcc gacgtcaaca taactttgga tttcatggct gtctcttcct atggggattc
     1741 cacgaagacc agcggtattg ttcgtattgt aaaggatctg gatggcagca tcaaagacaa
     1801 agatgtactg ctggtagagg atattgttga caccggactc accctgtcat atcttgtgaa
     1861 gatgatgcgg gaacgccaac cgaagagcgt tcgtgtgtgc gcactgctcg ataaagagga
     1921 acgacgtaag gtacatgtgg atgtcgatta cagaggcttt cccataccgg acgagttcgt
     1981 cgtagggtat gggcttgatt atggcggacg ctggcgtcat ctgcccgcta tttatacaat
     2041 cgaacagaag taactattta caatactatt tttagtcagg aggcggctat aatttgggcc
     2101 gattggtaaa aaatcttggt ttatacctca tactcattgt acttgtcgtg agtctggtca
     2161 acgtatttct ctcgccgaca caggggcctc agcaagttca ggagataggg tacagcacgt
     2221 ttctctccga agttacagcc gggagagttt caaatgtcct ggtacgcgaa aactccatca
     2281 aaggcagatt ttccgacggt aaagagtttg tctcatacgt ggtcggaata ggcgatttgg
     2341 cgaaggaagt cgctcaaaag ggcgtcgccg tggaggttga acccccgcaa cgaactcctt
     2401 ggtgggcaaa tatgctctct tcactcttcc cgaccctgct gctgattggc gtgtggattt
     2461 tctttcttta caacatgcag ggcgggggcg gcaaggtgat gaacttcgcc aaaagcaagg
     2521 cgaagctctt cattgacaac cgcccgaaag tcacatttaa agacgtcgcc gggtgcgacg
     2581 agtcgaaaga agagctttca gaagtcgtct actatcttcg ggatccttcc cgtttcaccg
     2641 ccctcggcgc cagagtccct cgcggagttc tgctgcttgg acctcctggt accgggaaaa
     2701 cgctgctcgc ccgtgcttgc gccggcgaag cggatgttcc ttttttcagc gtgagcggat
     2761 cggacttcgt cgagatgttc gtcggcgtag gagccgcccg tgttcgggat cttttcgagc
     2821 aagccaggaa atatcaaccc tgtatcattt ttatcgacga aatggatgca gttggccgtc
     2881 acagaggcgc tggactcggc ggaggacacg acgaacgcga gcagacgctc aaccagcttc
     2941 tggttgaact tgacgggttt gacgagacta cggggataat cctgatcgca gctaccaacc
     3001 ggcccgatat cctcgacccg gcgttgctgc gccccggtcg tttcgacaga catatcgttg
     3061 tcgacagacc cgacgttaaa ggacgagagg ctattcttga cgttcacgtc cgcgaaaaga
     3121 agcttgcgaa cgacgtcgac cttgccgtcc tcgcgcgccg aacaccgggt tttgtcggcg
     3181 ccgatcttgc caaccttgta aacgaagctg cgcttttagc cgcacgcacg ggtgaaaaag
     3241 agatcaaaat gaaagatttc gaagagggca tcgaccgtgt catagccggc ccggaaagga
     3301 agagtcgcct cgtcagcgat aaagagaaac taataatcgc ataccatgaa acggggcacg
     3361 cgcttgttgc gaaacgaatt ccttcatgcg atccggttca taaaatatcg ataatccccc
     3421 gaggacacat ggcattgggg tacacgctcc agctccccga ggaggatcgc ttcctcatgt
     3481 cgaaaaacga actcctcgac aaaatttgcg ttcttttcgg cggcagagtt gccgaggaac
     3541 tcaggttcgg ggacgtaact accggtgcga gcaacgacct cgaacgggcg acacagattg
     3601 cccgacaaat ggttaccgaa ttcggcatga gcgagaagct cggacttgta aaactgggac
     3661 ataaacacaa tgaagtattc cttggcagag atatcggaga agaaagaaac tacagcgacg
     3721 cgatcgcgta cgctatcgac caggaagtca aggcaatcat agactcctgt tatgagaaag
     3781 tccgaaacat ccttgtggag aactcggaag aaatggaaaa aatagcaaaa acacttctcg
     3841 aaaaagagat cattgagggg aaggaactca acgctcttct tggtctggag aatcctgaag
     3901 aagaaaaggt tgtaggacca gaagaagaag aacgcactga gacgcaagag accatcgccg
     3961 aagaaaatcc tgtcggcgaa agtaagaaca gcgacgccgc aaccaacgcg gctatataac
     4021 ggaaaaactg ggaataccgt gtcttcagaa ggatccggga acgggcgcaa cgctctcccc
     4081 ggatccctct tttctttcac ttgggggtgg attccgtatg ttcattgaaa agaagtttga
     4141 actccactct gattgggccc cctccgggga tcagccgcaa gcgattgaaa cgctcgcggc
     4201 tggtgtcgcc caaggccttc gattccagac ccttatgggt gtgacaggaa gcggaaagac
     4261 gttc
//
LOCUS       DAOTIL010000124         4263 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminobacteriaceae bacterium isolate AMR_MDS_3026
            NODE_24584_length_4263_cov_2.856226, whole genome shotgun sequence.
ACCESSION   DAOTIL010000124 DAOTIL010000000
VERSION     DAOTIL010000124.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868050
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminobacteriaceae bacterium (wastewater metagenome)
  ORGANISM  Aminobacteriaceae bacterium
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae.
REFERENCE   1  (bases 1 to 4263)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4263)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:19:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,224
            CDSs (total)                      :: 3,198
            Genes (coding)                    :: 3,184
            CDSs (with protein)               :: 3,184
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4263
                     /organism="Aminobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_24584_length_4263_cov_2.856226"
                     /isolate="AMR_MDS_3026"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:3034609"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(171..1247)
                     /gene="corA"
                     /locus_tag="P5201_03725"
     CDS             complement(171..1247)
                     /gene="corA"
                     /locus_tag="P5201_03725"
                     /inference="COORDINATES: protein motif:HMM:TIGR00383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015087 - cobalt ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0015095 - magnesium ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006824 - cobalt ion transport [Evidence
                     IEA];
                     GO_process: GO:0015693 - magnesium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium/cobalt transporter CorA"
                     /protein_id="HRV97671.1"
                     /translation="MSGGGKRGTGRRAKLPGLPTEKQLVAEERKQTTSGIEIMHYAPD
                     GFEEIRNATLEDCARLKDCPGITWINVDDIRDTGAVRSLAALFGLHPLTTEDIGNPAQ
                     RPKAENFEQYLFFALKMLTYDDATHSILNENVSVVLGDRWVVSFQEREGDVLDPVRDR
                     IRSGKGRLRSEGADYLAYAIMDSVVDEYFVVLEKLGDHIEEIDEQILAVPDRTHMKEL
                     HRLKREILFLRKAVWPLREEISTIEKSGSRLVGDSIKPFLRDLYDHTIEIIDMVETNR
                     DIVSGMHDTFLSAVSNRMNEIMKVLTIIGTIFIPLTFIAGVYGMNFEHMPELKWPRGY
                     YGVMAFMAALALSMLAAFKKRKWL"
     gene            complement(1244..1609)
                     /locus_tag="P5201_03730"
     CDS             complement(1244..1609)
                     /locus_tag="P5201_03730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF488 domain-containing protein"
                     /protein_id="HRV97672.1"
                     /translation="MTLLLKRVYEAPNPEDGFRILIDRLWPRGITKEKAAVDLWLKEA
                     APSPELRKAFCHDPEKWDDFRARYVAELETNAAVDTIMERLREGTVTLVYAAKDETYN
                     HAVVLAEHLERQIAVRNGA"
     gene            1785..3635
                     /locus_tag="P5201_03735"
     CDS             1785..3635
                     /locus_tag="P5201_03735"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_process: GO:0009966 - regulation of signal transduction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HRV97673.1"
                     /translation="MEELRELRERLLDAEGKLLETERFLADLIENSGSLMVIKSVDGV
                     YTFVNRRWEECTGLPRADVLGRTDAELFSPEAAEHFRRNDSEVLRRGELVEFEEFFDR
                     PDGRHFFLSGKFPVHGEDGTVSGLCAVITDITDRKRAEEKLRISRERYELAMAATNDG
                     LWDWNLVTGKVYFDPRYYTMAGYEPGEFPSSFDEWAKRLHPDDHPLCNRALQSYFSGK
                     TPAFDMEFRFRRKDGSWMWIRGRGRRVESDANGNAVRMVGTHIDITELKQQQQEIQYL
                     SFHDHLTGLYNRRFFDEELARLDTPRNMPLTLLLMDVNGLKLTNDAFGHQAGDRLLRT
                     VADVLRRTCREDEIIARIGGDEFILLLPQTGEEDAASLGIRLREAFENVRMETGTLSV
                     AFGWATKNDMSQPAGNVFITAENRMYQQKIYEMNSVRSKAISFILETLYENIPRERRH
                     SENVGVLSGRIGVAMDLDAEAVKELVLAGKLHDIGKIAIGAEILDKNGSLNESEWLTV
                     KRHPEIGYFILNSVSDYASLALIALAHQERFDGRGYPKGLSGGNIPLHARIIAVADAY
                     DAMTSGRPYRPPLSSAEALREIRAASGTQFDPSVVEVFLELFKKSPESIC"
     gene            3850..4182
                     /locus_tag="P5201_03740"
     CDS             3850..4182
                     /locus_tag="P5201_03740"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0004518 - nuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GIY-YIG nuclease family protein"
                     /protein_id="HRV97674.1"
                     /translation="MDRKKELLMEYKQRKREMGVFLFECAEGGRYIGRADDPKGAINK
                     NVFQLKMGSHPNKRLQKEWSDRGGAAFSVRVLDSLEYDEETKAECAEELAMLFDDWIQ
                     KMKDAEVV"
BASE COUNT          855 a         1246 c         1306 g          856 t
ORIGIN      
        1 cggcctgccg gttgtctggt tcgggcattc catggggtcg atcatcggcc tcaaggaaga
       61 gcagtacgag gagtacgccg agatgctgtt cgaaatcgcc aacatactgg cggacaaggc
      121 ggatatgttc ccgaggccgt tcctctgcgg cgtgtgagcc cgacgctcgg ctacagccac
      181 ttccgcttct tgaaggccgc gagcatgctc agggcgagcg ccgccatgaa cgccatgacc
      241 ccgtagtacc cccggggcca cttcagctca ggcatgtgtt cgaagttcat cccgtagacg
      301 cccgcgatga aggtcagcgg gatgaagatc gtcccgatga tggtgagcac tttcatgatc
      361 tcgttcatcc ggttgctgac cgccgagagg aacgtgtcgt gcatgccgct cacgatgtcg
      421 cggttggtct ccaccatgtc gatgatctcg atggtatggt cgtacaggtc gcgaaggaag
      481 ggctttatgg agtcgccgac gaggcgcgag ccgctcttct cgatagtcga aatctcctcc
      541 cgcagcggcc agactgcctt ccgcaggaag agaatctcac gcttcaggcg atggagctcc
      601 ttcatgtgcg tcctgtccgg aacggcgagg atctgttcgt cgatctcctc gatgtggtcg
      661 ccgagctttt caaggacgac gaaatactcg tccaccaccg aatccatgat ggcgtaggcc
      721 aggtagtcgg cgccttcgct ccggaggcgt cccttccccg accggatgcg atccctgacg
      781 ggatcgagaa cgtccccctc gcgctcctgg aatgagacga cccatcggtc gcccagcacg
      841 acgctgacgt tctcgttgag gatcgagtgc gtcgcgtcgt cgtaggtcag catcttgagg
      901 gcgaagaaga gatactgctc gaagttctcc gccttgggac gctgcgccgg gttgccgata
      961 tcctccgtcg tcagcgggtg cagtccgaaa agggccgcca ggctccggac tgcacccgtg
     1021 tcgcgaatgt cgtcgacgtt gatccatgtt attcctgggc agtctttcag ccgtgcgcag
     1081 tcttccagcg tcgcgttgcg gatctcctcg aaaccgtccg gagcgtagtg catgatctcg
     1141 atgccggaag tcgtttgttt ccgctcttcg gcgacgagct gtttctctgt tggaaggccg
     1201 ggcagcttcg ctcggcgtcc ggttccgcgc tttccgcctc cgctcatgcg ccgttcctca
     1261 ctgcgatttg ccgctccaga tgctccgcaa gcacgacggc gtggttgtat gtctcgtcct
     1321 tcgcggcgta gacgagcgtg accgtcccct cgcgcaggcg ctccatgatc gtatcgaccg
     1381 ccgcattcgt ctccagctcg gcgacgtagc gcgcccggaa atcgtcccac ttttcggggt
     1441 cgtggcagaa cgctttccgc agttccgggc tgggcgcggc ctccttcagc cacagatcga
     1501 cggcggcctt ctcctttgtg attccgcgcg gccacagccg gtcgataaga atccggaagc
     1561 cgtcctccgg atttggcgct tcgtacaccc gtttcagcag cagcgtcatg ctcttccctc
     1621 cccgcaggtc tcatgaaacg attatagcac cgggtttcct cgaaggcgga tgcgctgctt
     1681 cttgagatgt gagtatttgc gtcttgcgac tcattgcaaa aggcgccccc gtgcgattat
     1741 tataattatg cagcaagaaa gcgtgtccgg atgggggaga gcgaatggag gaactgcgag
     1801 agttgcggga acgccttctc gatgctgaag gcaagttgct cgaaacggaa cgtttcctcg
     1861 ctgatctgat agagaacagc ggctcgttga tggtcatcaa gagcgtcgac ggagtgtata
     1921 cattcgtgaa ccgcagatgg gaggagtgca ccggtctgcc cagggccgac gtgctgggaa
     1981 ggacggacgc cgagctcttc tccccggaag cagccgaaca cttccgccgc aacgactccg
     2041 aagtgctccg cagaggagag cttgtcgagt tcgaggaatt tttcgaccgt cccgacggca
     2101 gacatttctt cctctcgggc aaatttccgg tgcacggaga ggatggaacc gtctccggac
     2161 tctgcgccgt tatcaccgat atcaccgacc gcaagagggc ggaggagaag ctgcgcataa
     2221 gccgtgagcg ctacgagctg gcaatggcgg cgaccaacga cggcctgtgg gactggaacc
     2281 tggtcacggg caaggtgtac ttcgaccctc ggtactacac gatggccggc tacgagccgg
     2341 gcgagtttcc ctcctcgttc gacgagtggg cgaagcggct gcatccggac gaccatcctt
     2401 tatgtaatcg cgctcttcag tcgtatttct ccggaaagac gcccgcgttc gacatggagt
     2461 tccgctttcg ccggaaggac ggctcgtgga tgtggatccg cgggcgggga cggcgcgtcg
     2521 agtccgacgc gaacggcaat gccgtccgga tggtcgggac gcacatcgac atcaccgagt
     2581 tgaaacaaca gcagcaggag attcaatacc tcagctttca cgaccacctc accgggctgt
     2641 acaaccggcg cttcttcgac gaagagcttg cgcgcctgga cactccgaga aatatgccgc
     2701 tgacgctcct ccttatggac gtcaacgggc tcaagctgac gaacgacgcg ttcggccatc
     2761 aggcggggga ccgcctgctg cgcaccgtgg cggacgtgct ccgccgaacc tgcagggagg
     2821 acgagattat cgcccgcata ggaggcgacg agttcatcct tctgcttccc caaaccggtg
     2881 aagaggatgc cgcgtcgctc ggcatacgat tgcgggaggc ctttgagaat gtcaggatgg
     2941 agacggggac tctttccgtc gcattcggct gggcaaccaa gaacgacatg tcgcaacctg
     3001 cgggaaacgt cttcatcacc gctgaaaaca ggatgtacca gcagaaaatt tacgagatga
     3061 acagcgtccg cagcaaagcc atctccttca ttctggagac gctgtacgag aatattccac
     3121 gggagcgccg gcactcggag aacgtcggcg tcctctccgg gcggatcggc gttgcaatgg
     3181 acctggatgc cgaggcggtc aaagagctcg tactggcagg aaagcttcat gacataggca
     3241 agatagccat aggcgccgaa atcctcgata aaaacggttc cctgaacgaa agcgagtggc
     3301 tgaccgtaaa gcggcacccg gagatcgggt atttcatact gaactcggtg agcgactacg
     3361 cttcgctcgc cctcattgcg ctggcccacc aggagcggtt cgacggaagg ggctatccga
     3421 aaggactgag cggcgggaac attccgctcc atgcgcgcat catcgcggtg gcggacgcgt
     3481 acgacgcgat gacgagcggc cgcccctacc gtcctccgct ctcttccgcc gaggcgttgc
     3541 gtgaaattcg cgccgcgtcg ggaacacagt tcgacccgag cgtcgtcgag gtgttcctgg
     3601 agctcttcaa aaagtccccg gaatccatct gctgacgcac gtcgtcggtt gcatcacggc
     3661 cggggcgcgc ctttccctcc ctcttccccc acccgcatct ttccactgat cgtcgagcaa
     3721 cacggctgaa aaactggttt ttttccgccg gagcgcttga cgtcttcggc agatgtgata
     3781 ctattcatcc gtgataatta gtaaacgagt aatttagtaa atgactcgtt ggattcgagg
     3841 tgtcgtatcg tggacaggaa aaaagagctg ctgatggagt acaaacagcg gaagcgggag
     3901 atgggcgtct ttcttttcga atgcgcggag ggcggacgct acatcgggcg cgccgacgac
     3961 ccgaagggcg cgatcaacaa aaacgtcttt cagctgaaga tggggtcgca cccgaacaag
     4021 cgcctgcaga aggagtggtc ggaccgcggc ggggcggcgt tctccgtccg cgtgctggat
     4081 tcgttggagt acgacgagga gacgaaagcg gagtgcgccg aagagcttgc gatgctcttc
     4141 gacgactgga tacagaaaat gaaggatgcg gaggtcgtat aatatgaaaa tcccgacgac
     4201 attgaaacat aaaccggtgg tcgcggtgga gaactacgaa aacgtggacg gacgccaggc
     4261 gta
//
LOCUS       DAOTIL010000125         4239 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminobacteriaceae bacterium isolate AMR_MDS_3026
            NODE_24751_length_4239_cov_3.973948, whole genome shotgun sequence.
ACCESSION   DAOTIL010000125 DAOTIL010000000
VERSION     DAOTIL010000125.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868050
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminobacteriaceae bacterium (wastewater metagenome)
  ORGANISM  Aminobacteriaceae bacterium
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae.
REFERENCE   1  (bases 1 to 4239)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4239)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:19:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,224
            CDSs (total)                      :: 3,198
            Genes (coding)                    :: 3,184
            CDSs (with protein)               :: 3,184
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4239
                     /organism="Aminobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_24751_length_4239_cov_3.973948"
                     /isolate="AMR_MDS_3026"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:3034609"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2032)
                     /gene="purL"
                     /locus_tag="P5201_03745"
     CDS             complement(<1..2032)
                     /gene="purL"
                     /locus_tag="P5201_03745"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005662407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004642 -
                     phosphoribosylformylglycinamidine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase
                     subunit PurL"
                     /protein_id="HRV97675.1"
                     /translation="MEYTQAGLRETEYEEIRRVLGREPNECELRIMGVMWSEHCSYKS
                     TKNLLKLFPNNGDRVVLGPGENAGIIDLGDGLGAAFKAESHNHPSAVAPYQGAATGVG
                     GIIRDILALGARPVASMDGLFFGEPSSPRTQSLASGIVHGVGGYGNAVGVPTVGGKTA
                     YDPSYNENPLLNAFCIGLIPLDEIVSSRTARPGQAVVLLGSKTGRDGIAGAAFASVEL
                     SEDNKASRPSIQIGDPFGEKLLIEACLEMNEKKLIVSMQDMGAAGIISSSSEVAAKSG
                     VGMILHFDKIPLRAENMEPWEIALSESQERMLLIVDRSYLHEVMEVAEKWELDCAEFG
                     ETVEGDEYTILFHGERAASLPATLIGDRCPLIPWPSRRPADLEKKWEFDVDDIPLPED
                     WNETLLSLLADPSLASKKRIYEQYDSMVQLNTVIGPGHPVSMLRIKGRDSLIAMTLDA
                     DPWKCSLDPFRGGAETVARTVRDLAVAGARAAGLTDCLNFPSPEVPEQYWVLEECVKG
                     MAEACGAFDCPVVSGNVSLYNESPDGAILPTPVVGTVGIIPSIDAALSAGTWEEGDVL
                     FLVGPCNPSLAGSRYLLSQGKLAGRPLPFYPEAEKDFVERALKTASAKAAHSGRALSG
                     GGLAAALAKESMESGIGASISLSIPTRKDILLFGEGCARALYAVPMSRVPLFK"
     gene            complement(2034..2738)
                     /gene="purQ"
                     /locus_tag="P5201_03750"
     CDS             complement(2034..2738)
                     /gene="purQ"
                     /locus_tag="P5201_03750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006583653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004642 -
                     phosphoribosylformylglycinamidine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase
                     subunit PurQ"
                     /protein_id="HRV97676.1"
                     /translation="MNTAVIVFPGSNCDQDVMHAVAGSLGTKVRAVWHKETTLPEDTD
                     FVILPGGFSFGDYLRCGAMAARSPIMKAVAEHGRRGGLLLGICNGFQILTESRLLPGA
                     LLPNRTINFVCRPCFLRVESVSTPFTSLFSKGQVVQFPIAHGDGLYTLPEEELKALED
                     RGGVVFRYSDSDGLTGDNANPNGSANNIAGIVNERGNILGLMPHPERASEALTGGEDG
                     RVFWQSILESFTKGGR"
     gene            complement(2739..2990)
                     /gene="purS"
                     /locus_tag="P5201_03755"
     CDS             complement(2739..2990)
                     /gene="purS"
                     /locus_tag="P5201_03755"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009164886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase
                     subunit PurS"
                     /protein_id="HRV97677.1"
                     /translation="MTYRVSIFIELQEGVLDIQGKAVERSLAGMGHDALRSVKVGKYI
                     QVWVETGSAEDARKEAERMCDDLLVNSVIEQFRIEVEGE"
     gene            complement(2987..3715)
                     /locus_tag="P5201_03760"
     CDS             complement(2987..3715)
                     /locus_tag="P5201_03760"
                     /EC_number="6.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006583651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004639 -
                     phosphoribosylaminoimidazolesuccinocarboxamide synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylaminoimidazolesuccinocarboxamide
                     synthase"
                     /protein_id="HRV97678.1"
                     /translation="MEKKKFLYEGKAKKIYDTDDVNLCIIEYKNSLTAFNALKKDTIE
                     GKGELNNLISSSLFELLSEKGIPTHFVKRVDNIHQIVKKVSIVPIEVVVRNITTGSLC
                     KRLGVEEGKVLPRPLVELYLKDDALGDPLITEDHALLFGWSTEEQLLKIKEIALKVNS
                     VLSEYFTGLRIILVDFKLEFGVDAEGRLLLADEVSPDTCRFWDRDTKNRLDKDRFRKD
                     LGDVLGAYQEIWRRISSSNEDGAR"
     gene            3817..>4239
                     /locus_tag="P5201_03765"
     CDS             3817..>4239
                     /locus_tag="P5201_03765"
                     /EC_number="2.7.7.77"
                     /inference="COORDINATES: protein motif:HMM:NF024214.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0061603 - molybdenum cofactor
                     guanylyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum cofactor guanylyltransferase"
                     /protein_id="HRV97679.1"
                     /translation="MSNFIEEFPGTTLILAGGQGSRMGGEKLTLAVDSEPLLLKVIRR
                     VLPFSKDILLSVAPRQVSYVSTVLSGIISLYGIRTVEDRLPVEKSPSEVDEKRSGPLA
                     GIVSGLEACSSEWLFVCACDMPRVSEAVVRTLWKHGDKS"
BASE COUNT          924 a         1234 c         1129 g          952 t
ORIGIN      
        1 ctttaaaaag aggaaccctg ctcattggaa ccgcataaag cgcgcgtgcg cagccttcgc
       61 cgaagagcaa aatatctttg cgcgtaggga tggaaagaga aatagaagcg ccgataccgc
      121 tctccatgct ctctttggca agggctgccg caagcccccc tcccgaaagg gcgcgcccgc
      181 tgtgtgcggc ttttgcggac gcagtcttca gggctctctc aacaaagtct ttttcagctt
      241 ccggatagaa ggggagcggg cgtcctgcaa gctttccttg tgagagaagg tagcgactcc
      301 cggcgagcga tggattgcac gggccgacga ggaagagaac atcgccctct tcccacgtac
      361 cggccgaaag cgcagcatcg atcgacggaa taatcccgac tgttccaacc acgggagtcg
      421 ggaggatggc gccgtcgggg ctttcgttgt ataaactcac atttcccgag accacaggac
      481 agtcaaaggc gccgcacgcc tcggccatcc ccttgacgca ctcttcgagc acccagtact
      541 gctccggaac ctccggggaa ggaaagttca gacagtcggt aagccctgcg gcgcgtgcgc
      601 cggcgactgc gagatcacgg acggttctcg cgacagtttc ggccccgccg cggaaaggat
      661 caagcgaaca cttccaggga tcagcgtcaa gagtcattgc aatgagcgaa tcccgcccct
      721 taatcctgag catgctcaca ggatgccccg ggccgataac agtgttcagc tggaccatcg
      781 aatcgtactg ctcgtagatc cttttcttcg aagcgagcga aggatctgct agaagagaga
      841 gcaacgtttc gttccagtct tcgggaagag gaatatcatc gacgtcgaat tcccatttct
      901 tttcaagatc cgcagggcgt cgggaaggcc agggaataag agggcaccgg tctccgatga
      961 gcgtcgcagg gagtgaagcg gctctctccc cgtggaagag aatcgtgtac tcgtcacctt
     1021 cgactgtctc gccgaattcg gcgcagtcca gctcccactt ttccgcaacc tccatcacct
     1081 catgcagata agaacggtca acgatcagca acatgcgctc ctgcgattcg gagagagcga
     1141 tctcccacgg ttccatattc tccgcccgga gaggaatctt atcaaagtgc aaaatcatcc
     1201 cgacgccgct tttcgccgcg acttcgcttg aagaggagat aatccccgcc gcacccatgt
     1261 cctgcatact cacgataagt tttttctcat tcatttcgag gcacgcctcg atcaggagct
     1321 tttctccgaa cggatcgcct atctggatcg atggtctgct cgccttgtta tcctcggaga
     1381 gctctaccga ggcgaacgca gctccggcga tcccatccct tcccgtcttc gagccgagca
     1441 ggacgaccgc ctgccccgga cgcgcggtgc gggaactgac gatttcgtcc agcggaatta
     1501 gtccgatgca gaatgcgttc agcagcgggt tttcgttata ggacggatca tatgccgtct
     1561 ttccgccgac agtaggcacg ccgacggcat tcccgtaccc tccgacaccg tgaacgatac
     1621 ccgatgcaag cgactgcgtc ctgggggacg aaggctctcc gaagaagagt ccgtccatcg
     1681 aggcgaccgg tcgtgcgccg agcgctagaa tatcacggat aattcctccc acaccggtgg
     1741 ctgctccctg gtacggggca accgctgaag gatgattgtg actctccgcc ttgaacgccg
     1801 ccccgagccc gtctccaaga tcgatgattc cggcgttttc gcccggtccg agaaccacgc
     1861 gatctccgtt gttcggaaag agcttcagca agttcttcgt cgacttgtag ctgcagtgct
     1921 cggaccacat gacccccatg atgcggagct cgcattcatt cggctccctg cccaggacgc
     1981 ggcggatctc ctcgtattcg gtctcgcgaa gaccggcttg tgtgtactcc atttcatcgg
     2041 cctccctttg taaacgattc gaggatagat tgccagaaaa cccggccgtc ctctccgccg
     2101 gtaagcgctt cgctcgccct ttcggggtgc ggcataagac caaggatatt accccgctca
     2161 ttgacgatcc cggcgatgtt gttcgccgat ccgttgggat tcgcattgtc gccggtcagc
     2221 ccgtcggaat cgctgtagcg gaagacaacg cccccgcgat cttcaagagc cttcagctcc
     2281 tcttcaggca gcgtgtacag tccgtctccg tgcgcaatag ggaactgaac gacctgcccc
     2341 ttcgaaaaga gcgaggtaaa cggcgtcgat acgctctcca cacgcaagaa acaggggcgg
     2401 cagacaaagt tgatcgttcg gttcggaagc agcgcgccgg gaagcagccg tgactctgtc
     2461 aaaatctgga acccgttgca gattcccaac agcagacctc cccggcgccc atgttcggca
     2521 accgccttca taatagggga acgagccgcc atcgcgccgc agcgaaggta gtcgccgaag
     2581 gagaaccccc cggggagaat aacaaaatcg gtgtcttcgg gaagcgtcgt ctctttgtgc
     2641 cagacggcac ggacctttgt cccgaggctc ccggcgacgg cgtgcatgac atcctggtcg
     2701 cagttgctgc cgggaaagac gatgaccgcc gtgttcactt actccccctc cacttcgata
     2761 cggaactgct cgatgacact gtttaccagc aggtcgtcgc acattctctc ggcttctttt
     2821 cgtgcgtcct ccgcagaccc ggtttccacc cacacctgaa tgtacttacc gaccttcacc
     2881 gaacgcaacg cgtcgtgccc cataccggca agagaacgtt caaccgcttt cccctggata
     2941 tcaagcacac cttcctggag ctcgataaaa atggaaaccc ggtaggtcat cgcgccccat
     3001 cctcattcga ggaggaaatt cttctccata tttcctgata cgctccaaga acatctccca
     3061 agtccttccg gaaacggtct ttgtcgaggc ggttcttcgt gtccctgtcc cagaaacggc
     3121 aggtatcagg cgagacttcg tctgccagaa gcaagcggcc ttcggcatcg acgccgaact
     3181 caagtttgaa atcgacgagg ataatacgca atcccgtgaa gtactccgaa agaacgctat
     3241 tgaccttcag agcaatttcc tttattttta aaagctgttc ctcggtgctc catccaaaaa
     3301 gcagcgcatg atcttcggtg ataagcggat ctccgagggc gtcgtccttc agatagagtt
     3361 ccacaagggg acggggcaag actttccctt cctcgactcc aaggcgcttg cacagcgagc
     3421 cggtggtaat gttgcggaca acgacttcga tcggaacgat ggatactttt tttacgatct
     3481 ggtgaatgtt gtcgactctt ttgacgaagt gcgtgggaat gcctttttcg gagaggagct
     3541 cgaagagaga agagctgatc agattgttaa gttcgccttt gccttcgatg gtatcttttt
     3601 tcagggcatt aaatgctgtg agggagtttt tgtactctat aatacagaga ttcacatcat
     3661 ccgtatcata aatttttttc gctttgcctt cgtaaaggaa cttctttttt tccaaggtgg
     3721 ggcacctcca aggattaata tacttacatt attcatcgac attgaaaaaa tgtcaacagt
     3781 aaattgctcc gcaattttcc cccggaaggt gattttgtga gtaattttat cgaggagttt
     3841 ccgggaacaa cattgattct cgcagggggg cagggttcga gaatgggcgg tgaaaagctt
     3901 acgctcgccg tcgactccga gccgcttttg ctgaaggtta ttcggagagt tctccccttt
     3961 tcgaaggaca tcctgctttc agtcgccccc cgccaagttt cgtatgtttc cactgtgttg
     4021 tcgggtataa tctctctgta cggcattcgt accgtcgagg atcgccttcc tgtcgagaaa
     4081 agcccttcgg aggttgatga aaagagaagc ggcccgctgg caggcatcgt ttccgggttg
     4141 gaggcgtgtt cttcagaatg gctttttgtc tgcgcctgtg atatgccgcg agtctcggag
     4201 gccgtcgtac gaacgctctg gaaacacggc gacaagagc
//
LOCUS       DAOTIL010000126         4236 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminobacteriaceae bacterium isolate AMR_MDS_3026
            NODE_24772_length_4236_cov_3.532169, whole genome shotgun sequence.
ACCESSION   DAOTIL010000126 DAOTIL010000000
VERSION     DAOTIL010000126.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868050
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminobacteriaceae bacterium (wastewater metagenome)
  ORGANISM  Aminobacteriaceae bacterium
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae.
REFERENCE   1  (bases 1 to 4236)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4236)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:19:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,224
            CDSs (total)                      :: 3,198
            Genes (coding)                    :: 3,184
            CDSs (with protein)               :: 3,184
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4236
                     /organism="Aminobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_24772_length_4236_cov_3.532169"
                     /isolate="AMR_MDS_3026"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:3034609"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..849)
                     /locus_tag="P5201_03770"
     CDS             complement(<1..849)
                     /locus_tag="P5201_03770"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRV97680.1"
                     /translation="MKPNSPAAGSVSLFLNIFGRCDFLFALLLGNAGQVLAVNDRAAA
                     LLGTEENCCPLPLAELLAEGQEDCLARIEEAASGESLPLSLKTAAGSRIGIDCSVAAL
                     GEEMLVFGMYSDQENDRIITEMSVLNLEMANMTRELHKKNLQLAVSQQALAKADSMKK
                     TFLAIIAHDLRGHVGGVSQLLSMLQQDYDDMDDAEKKECVTLASDEARRTFLLLEDLL
                     SWSRSQLDQIEFTPVDCNLRALIEENIALLQSRAKEKGVALSHETPPSLSATLDRSMM
                     GTVLRNL"
     gene            complement(836..1954)
                     /locus_tag="P5201_03775"
     CDS             complement(836..1954)
                     /locus_tag="P5201_03775"
                     /inference="COORDINATES: protein motif:HMM:NF014375.2"
                     /note="Presence of a B(12) (cobalamin)-binding domain
                     implies dependence on cobalamin itself, in one of its
                     several forms, or in some unusual lineages, dependence on
                     a cobalamin-like analog; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0031419 - cobalamin binding [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cobalamin-dependent protein"
                     /protein_id="HRV97681.1"
                     /translation="MASALETSALVPVSQKAAQDYAAHADNLLNYVDACMKKAPLDAL
                     AAKQVEMMLQNHRNHLQFMMSFFRLNNPELLKEIVIWVYRAYQAHGFSPDYFPAVLRR
                     WMEAVSQFLPPESAPEILGVYGWMLENHQRFLAAADEYRHMEQEAAGAPHPLQALRNE
                     FLQGLIAGSLSRCNACATGYITGKDELREFYLGVVQPAMIEIGNLWERGELSVAREHL
                     ATAIVTRIMAAQHMRFFGLEKPKGKALISAAANEFHELGARMVADLFDLDGWDVTYIG
                     SNVPVEAAEEMIRNEKPSVVGLSVTMPFNLGKTADLVDRIHSSPELAGVKIIVGGRAF
                     QFSPTLWQTIGADAYANNAIEALNAANSFMETRPREAE"
     gene            complement(2174..3316)
                     /locus_tag="P5201_03780"
     CDS             complement(2174..3316)
                     /locus_tag="P5201_03780"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRV97682.1"
                     /translation="MARYGIRRVAWLCRGGIVLFGILAFIVLLLPLVSEAQQGLSASE
                     REYVERYNTLTPQQQQSLRDRMTPDQRTHLDRLLLMLSSPDRSGGASMTPAISAAAPS
                     GAQAKLTKKIIAGVPTQLTYTVVVQNRSGVPVVNPAVKDNGKPLNNDDAVKTGGNRDN
                     ILEIGETWTWTYTVTVTGKTGERILNTVTVEGPDSTIRDTDQGNNTDETVVEVDPAPG
                     NYDLSVSKTVAVTGQADTGLPPGINPTDVVDSPPQTITPDDCGKWLRSASGGYKGTRK
                     QYDIASLPSGTTFDLDFQAFDVPDKIEVYYPAGTKVFDSGWRGDMNHIQKKPSVANLY
                     PGGVVGTGKLLQNGLFTKSGANEFLVIVYGPEQGTAWEFSVKANCP"
     gene            complement(3427..4038)
                     /locus_tag="P5201_03785"
     CDS             complement(3427..4038)
                     /locus_tag="P5201_03785"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF012521.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HRV97683.1"
                     /translation="MESTLYLVRHGRPELPDNEMRFLGWTDLPLSSEGRRQAEATRDA
                     LSGYALDRIVHSGLKRSAETAGILREGRETPFEEISAFRELAFGEWELRSMKELAENE
                     PELFAARGRDFARFRPPGGENFLDLHERVWPAFLELLGREKGNIMLVAHAGVIKTIIF
                     TLLGISWQKLFSLRQDYCGVHVISRWDEHLTVRQLNWTPRIGG"
BASE COUNT          811 a         1238 c         1247 g          940 t
ORIGIN      
        1 cagattgcgg agcaccgttc ccatcatgct tctatccagc gtggcggaaa gggaaggcgg
       61 cgtctcgtga gagagggcga ctcccttctc cttcgcgcgg gattgcagca gggcgatgtt
      121 ctcttcgatc agcgcgcgca ggttgcagtc gacgggggtg aattcaatct ggtcaagctg
      181 tgatctcgac caggagagga ggtcttcgag aagaaggaac gttctgcggg cttcgtcgga
      241 ggcaagcgta acacactcct tcttttccgc gtcgtccatg tcgtcatagt cctgctgaag
      301 catcgagagc agttgactta cgcctcccac atgacctctg aggtcgtggg cgatgatggc
      361 aagaaacgtt tttttcatcg aatcggcctt tgccagcgcc tgctgggaga cagcgagctg
      421 aaggtttttc ttgtggagtt ccctggtcat gttggccatc tccaggttca agaccgacat
      481 ctctgtaatg atccggtcgt tctcctgatc cgagtacata ccaaagacga gcatctcctc
      541 accaagcgcg gcgacgctgc agtcgattcc gatgcgcgat cctgccgccg tcttcaggga
      601 gagcggcaga ctttcgccgg aagccgcttc ttcgatgcgc gcaaggcagt cctcctgtcc
      661 ttccgcgagg agttccgcca aaggcagggg gcagcagttc tcttctgttc ccagaagggc
      721 ggcggctctg tcgttgaccg cgagaacctg tcccgcgttc ccgagaagca gcgcgaagag
      781 aaagtcgcac cgtccgaaga tgttcagaaa gagagagacg ctgcccgcgg cagggctatt
      841 cggcttcacg tggccgtgtc tccatgaaac tgtttgccgc gttcagggct tcgatggcgt
      901 tgttcgcgta tgcgtctgcg cctattgttt gccataaggt cggcgagaac tggaacgccc
      961 tgccgccgac gatgatcttc acgcccgcaa gctccggcga ggagtgaata cggtcgacca
     1021 ggtcggctgt cttcccgagg ttgaagggca tcgtcacgga gaggcccacg acggacggct
     1081 tctcgttgcg gatcatctct tccgccgcct ccacggggac gttgctgccg atgtacgtca
     1141 catcccatcc gtcgaggtcg aacaggtcgg cgaccatgcg cgcgcccagc tcgtggaact
     1201 cgttggccgc ggctgaaatc agcgccttcc ccttgggttt ctccagaccg aagaagcgca
     1261 tgtgctgcgc cgccatgatc ctggtgacga tggctgttgc gagatgttcc cgcgctaccg
     1321 aaagctcgcc ccgttcccac agattgccga tctcgatcat cgccggctgc acgacgccga
     1381 ggtaaaactc ccgcagctcg tcttttccag tgatataccc ggtagcgcag gcattgcagc
     1441 gggagagact gcccgcaatc agcccctgga gaaactcgtt ccggagcgcc tgcagcgggt
     1501 ggggcgctcc ggcggcctct tgttccatgt gtctgtactc atcggcagcc gcgagaaagc
     1561 gctggtggtt ttccagcatc cagccgtaga cgccgagtat ctccggtgcg ctctccggcg
     1621 ggaggaattg cgaaaccgcc tccatccagc gtctgaggac ggcggggaag tagtcgggag
     1681 aaaatccatg ggcctgatac gcccgataga cccagataac aatctctttg agcagttcgg
     1741 ggttgttcag gcgaaagaac gacatcataa attggaggtg attgcgatgg ttctgcagca
     1801 tcatttcaac ctgtttcgcc gcgagagcgt caagcggggc ttttttcatg cacgcgtcga
     1861 cataattcag cagattgtcc gcatgcgccg catagtcctg cgccgccttt tgcgaaaccg
     1921 gaacaagagc gcttgtttca agtgcggatg ccatcgaact caactccttc tcgtggacga
     1981 aaatggatgg aacagctgtt atggtaggct ttttagaagt gggtgtcaat ccggcccgcg
     2041 tgttccgtat tcctttcccg gcctccggcg cggcatgctt cagggaaaag cccccggcgt
     2101 ccggagagtt ttggacgccg agggcttttt tcgaatttca tagcatctgt cctggagcgt
     2161 ttttctccca agactaggga cagttggcct tgacgctgaa ctcccaagcg gttccctgtt
     2221 ccgggccgta gacgatcacc aggaattcat tcgcgccgct cttcgtgaaa agaccgttct
     2281 gcagaagctt ccccgttccg accacgccgc ccggatagag attggccacg ctcggtttct
     2341 tttggatatg gttcatatcc ccccgccatc cgctgtcgaa aaccttggtt cctgcaggat
     2401 agtaaacctc tatcttgtcc ggcacgtcga aggcctggaa gtccaggtcg aaggtcgttc
     2461 ccgaaggcag agaagctata tcgtactgct tccgcgttcc tttgtagccg ccgctggcgc
     2521 ttcgcagcca ctttccgcag tcgtccggag tgatggtctg cggcggggag tcgacgacgt
     2581 ccgtcggatt aatgcccgga ggaagtccgg tatcggcctg tccggtgacg gcgacggtct
     2641 tcgagaccga aaggtcgtag ttccccggcg ccggatcgac ctccacgacg gtttcatccg
     2701 tgttgtttcc ctggtcggtg tcgcggatcg tagaatccgg tccctccacc gtgacggtat
     2761 tgaggatgcg ctctccggtt ttccccgtca cggtgactgt gtacgtccat gtccacgtct
     2821 cgccgatctc aaggatattg tcccggttgc cgcccgtttt cactgcatcg tcgttgttca
     2881 aaggtttacc gttgtctttt accgcggggt tgacgacagg gactccggac cggttctgta
     2941 caacgacggt gtaggtgagc tgcgtgggga cgcccgcgat tatttttttc gtcagttttg
     3001 cctgtgcacc cgagggagcg gcagccgata tcgcgggcgt catagaggcg cctcccgatc
     3061 ggtcagggga ggagagcatc aacaggagcc tgtccagatg cgttctctgg tcgggggtca
     3121 tcctgtcgcg aaggctctgc tgctgttggg gcgtcaacgt attgtaccgt tccacatact
     3181 cccgttccga agcggagagc ccctgctgcg cctcggaaac aaggggcaaa agcagtacaa
     3241 tgaaggcgag aatcccgaaa agtacaattc cccctctgca cagccacgcg acacgtcgta
     3301 taccataccg cgccatgatg acattcctcc tttcctttga atagtgcgtc tttacggaat
     3361 acaatagcaa taagcgcatc ttctgtcaag tcatctgtaa cggcgcgccc tttcgatagt
     3421 ttgctcttac cctccgatgc ggggcgtcca gttgagttgg cgtaccgtga gatgctcatc
     3481 ccagcgggag atgacatgca cgccgcagta gtcctgacgg agcgagaaga gcttctgcca
     3541 ggaaataccg agcagcgtga aaatgatcgt cttgatgacg cccgcgtggg cgacgagcat
     3601 gatgtttcct ttttcgcgac ccaggagttc aaggaaggcg ggccacaccc gttcgtgcag
     3661 atcgaggaag ttttcccctc ccggcggacg gaagcgcgcg aagtccctcc ccctcgccgc
     3721 gaagagttcc ggctcgttct cggccagctc tttcatggac cgcagctccc attcgccgaa
     3781 cgcgagctcc cggaatgcgg aaatctcctc gaagggagtc tcacgccctt cgcgcaggat
     3841 tccggccgtc tcggcggagc gcttcagccc gctgtggacg atgcggtcga gagcgtatcc
     3901 cgagagcgcg tctcttgtcg cctccgcctg ccgccgtccc tcggaggaga gcggcagatc
     3961 ggtccatccc agaaagcgca tttcattgtc gggcagctcc ggccgtccgt gccgcacaag
     4021 ataaagtgta gattccattt gaagactcct tccgaagcgg tgcatttcaa tgtcgtttta
     4081 cggtacaata acagaaacac gcatttcgtg aaaggagaac cgggaaaatg aacaacaaga
     4141 tgaccgacaa gactgttgtc gttacgtgcg ccgcatcgga gagtgtccgt agaggcgttg
     4201 agaaagggct cgacggcgcc ggaaaggccg tcttcc
//
LOCUS       DAOTIL010000127         4233 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminobacteriaceae bacterium isolate AMR_MDS_3026
            NODE_24797_length_4233_cov_3.520105, whole genome shotgun sequence.
ACCESSION   DAOTIL010000127 DAOTIL010000000
VERSION     DAOTIL010000127.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868050
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminobacteriaceae bacterium (wastewater metagenome)
  ORGANISM  Aminobacteriaceae bacterium
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae.
REFERENCE   1  (bases 1 to 4233)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4233)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:19:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,224
            CDSs (total)                      :: 3,198
            Genes (coding)                    :: 3,184
            CDSs (with protein)               :: 3,184
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4233
                     /organism="Aminobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_24797_length_4233_cov_3.520105"
                     /isolate="AMR_MDS_3026"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:3034609"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            2..544
                     /locus_tag="P5201_03790"
     CDS             2..544
                     /locus_tag="P5201_03790"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013048844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Maf family protein"
                     /protein_id="HRV97684.1"
                     /translation="MLHSLGWSFRIDVPDICEDLLPGEGPEEAARRLSRAKAAEAAAR
                     NPGALVVAADTLVAAGGVILGKPSCREESLRMLRMLSGAVHSVFTGVALRRGEEERFA
                     VEETRVEFRPLSEESMKLYVESGEGDDKAGAYAIQGRGALLVSSLHGDYFNVVGLPLC
                     RLSRLLEEFGCSLADQWRNS"
     gene            544..2517
                     /locus_tag="P5201_03795"
     CDS             544..2517
                     /locus_tag="P5201_03795"
                     /inference="COORDINATES: protein motif:HMM:NF039831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5693 family protein"
                     /protein_id="HRV97685.1"
                     /translation="MIKSTADFLETSRRNPAIFVALVLILLSAWALLPRLAAERESKD
                     AAIVLDYRDLATFSIREGKSPEMLWPLLSQNGASGLMVSELTGQQLSMGTLPLYYGPA
                     SGLPGAAVKLASGNPLRAAFFIPRTHPGAALLPPLLSARFRGAHVQEYETGTAVLLTR
                     SLEELFFTGVLPDIEGLLIAAKHDIPVFYRVAPALPSDTDASLATLGALLAAFPQIRT
                     VSPSGEVALGYPNLRPLAERIVKEGRSVAMVEFSRQIGAPQLNWLAYPRLVSLHSVTN
                     EEISSRNISRSALYERFVRAVKERAVRLLVFRPSAMEASDAPFERFIEELRTLASGIT
                     ASGAQLRWPEPFQPWRTGFVGALALALAFVFSMLRFLQRFFNGGTDDRKNAEDALAWR
                     TTPRGLAVFSAIVLLAGVIILLLPSAGRFAGALTAVFVVVEASFLALDGWRTPWLGII
                     GSFLFAVAGGLAIAAFFSDPASMLRLRSFSGVKATLFLPPLLVLLADLKRRVHPESLG
                     ELFRRPPLWGEIFLLVLLLGGAGLVLFRSDNVQFVPAFEVRMREFLERALVARPRNKE
                     VFLGYPALLLWYFVRKADMWPRYREVFRLATVIGFSSAVNSFCHFHTPLFFILFRQFN
                     GLWTGVLLGMISIVFLRFVIMPLWYRYGGLVTE"
     gene            2514..3551
                     /gene="csaB"
                     /locus_tag="P5201_03800"
     CDS             2514..3551
                     /gene="csaB"
                     /locus_tag="P5201_03800"
                     /inference="COORDINATES: protein motif:HMM:TIGR03609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046919 - pyruvyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0042545 - cell wall modification [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide pyruvyl transferase CsaB"
                     /protein_id="HRV97686.1"
                     /translation="MRPEREFRVALCGYYGFGNLGDELLAEALIAALERCGIKRGEMA
                     MLSASPEESARAHGVCSVSRWSPRAVFRILRRSETFLLGGGGLFQDVTSLRSPLYYWG
                     ALRLALLAGARPWCAGQSVGPLLTKRGRLLARSALGSCSVRGVRDARSAELLADWGMN
                     SVVTCDPVFSLTPPVTSPRHGSALLVNIRPWTGGLASKTAEESARLASSRSLSLTGFA
                     LAAEDAGIMEELRRSGVFPAERIVLLDAENWRSECGRLFPEADGVVAMRFHASLLALL
                     FGRPLTAVAYDPKVESLASEWSLPSWSGSGALPEPAAPLGPFRPFEKGAEFLKTFSEM
                     CAAAMRRDGKE"
     gene            3556..>4233
                     /locus_tag="P5201_03805"
     CDS             3556..>4233
                     /locus_tag="P5201_03805"
                     /inference="COORDINATES: protein motif:HMM:NF012670.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HRV97687.1"
                     /translation="MLREPSFLAEKASEIRRDVVRMIGLARSGRLASSLSIVDILAWL
                     YWDVLSIDPADPWFESRDRFVLSKGRGAPALYATLANRKFFERDELWSYRRLGAMLQG
                     YPQALRIPGVDAPGGGSFGMGLGIANGLAIALRRGRSPCRVFCLAGEEELQEGAFWEA
                     VIAASRFCLDSICLVIDRYRTKEEAGIPSASDEAFCEKIRSFGWTAEACDGHDFSSLG
                     GALRLLGQ"
BASE COUNT          639 a         1230 c         1361 g         1003 t
ORIGIN      
        1 gttgcttcat tccctggggt ggtctttccg gatagacgtc ccggatattt gcgaggatct
       61 gcttcccgga gaaggtcccg aggaggccgc gaggcgtctt tcgcgggcaa aggcggccga
      121 ggcggcggca cgaaatccgg gggcgcttgt agtggctgcc gacacgctgg ttgccgcggg
      181 cggcgttatc ctcggcaaac cgtcctgccg ggaggaatcg cttcggatgc ttcggatgct
      241 cagcggggcg gttcatagcg tcttcaccgg agtcgcactt cgccgtggag aagaggagcg
      301 gttcgctgtc gaggagacca gggtggagtt ccggcctctt tcggaggagt cgatgaaact
      361 ctacgtcgaa agcggcgagg gggatgacaa ggcgggcgct tacgcgatcc aggggcgggg
      421 cgcgttgttg gtgtcctcgt tgcacggcga ctacttcaac gtggttgggc tgccgctttg
      481 cagactgagc cgtttgcttg aagaattcgg ctgctctctg gccgatcaat ggaggaactc
      541 gtgatgatca aatccacagc tgattttctt gaaacttcgc ggcggaatcc cgcgattttt
      601 gtcgctcttg tactcattct cctctccgcc tgggctcttc tgccccgcct ggcggcggaa
      661 cgggagtcga aagacgccgc catcgttctc gactatcgcg acctggcaac cttctcgatc
      721 agagagggaa agagcccgga gatgctctgg ccgcttcttt cgcaaaacgg cgcttccggg
      781 ctgatggtgt cggagttgac ggggcagcag ctctctatgg ggacgctccc tctctactac
      841 ggcccagcct ccggccttcc gggagccgca gtgaagcttg catccgggaa tccgttacgg
      901 gccgcctttt ttattcctcg aacacatccc ggggcagccc ttcttccccc gcttttgagc
      961 gccagattca gaggggcgca cgttcaggag tacgagacag gaacggcggt gctgctcacc
     1021 aggagtctcg aagaactctt ctttaccggc gttctgcccg atatcgaagg gctgctgatt
     1081 gcggcaaagc acgatattcc cgttttctac agggttgcgc ctgcgcttcc gtcggatacg
     1141 gatgcttctc tggcgacgct cggagcgctt ttagcggcct tcccccagat tcgcaccgtc
     1201 tccccttccg gcgaagtcgc tcttggatac ccgaatcttc gcccccttgc cgaacgtatc
     1261 gtcaaggagg ggcgttccgt ggcgatggtc gaattttccc ggcagatagg cgctccccag
     1321 ctgaactggc tggcgtatcc ccggcttgtt tcgctgcata gcgtgaccaa cgaggaaatt
     1381 tcgagccgca acatctcccg tagtgcattg tacgaacgtt tcgtccgggc ggtgaaagag
     1441 cgcgcggttc gccttctcgt cttccggccg tcggccatgg aggcgtccga cgctccgttt
     1501 gaacggttta tcgaagagtt gcgtaccctg gcgtcgggta ttaccgcgtc gggcgcgcaa
     1561 ctccgctggc cggagccgtt ccagccatgg cggacgggat tcgtcggcgc ccttgccctt
     1621 gcgctcgcct ttgtcttttc tatgctccgc tttcttcaga ggtttttcaa cggcggaacc
     1681 gatgaccgga aaaacgctga agacgcgctt gcatggcgga cgacgccgag agggctcgct
     1741 gtcttttccg ctattgttct cctcgcgggt gtgatcattc tccttcttcc ttccgcaggc
     1801 cgctttgccg gagccctgac tgctgtcttc gtcgtcgtcg aggcgtcttt cctcgctctt
     1861 gacggctgga ggactccgtg gctcggcatt atcgggagtt tcctttttgc agtcgccgga
     1921 gggttggcca tagcggcttt cttcagcgat cctgcgtcga tgcttcggtt gaggagcttt
     1981 tccggggtga aagcgacgct cttcttgcct cccctgctcg tgcttttggc tgatctgaag
     2041 cggagagtgc atcccgaatc tctgggagag ctctttcgaa ggccgccgct gtggggtgag
     2101 atattccttc tggttctttt gctgggaggc gccggactcg ttctcttccg cagcgataat
     2161 gtccagttcg tccccgcgtt cgaagtccgt atgcgggaat tcctggaacg ggcgcttgtg
     2221 gcccgaccgc ggaacaaaga ggttttcctg ggctacccgg cgctcctcct ctggtatttc
     2281 gtccgcaagg cggatatgtg gcctcgctac cgcgaggtct tccgcctggc gacagtcatc
     2341 ggtttttctt cggctgtgaa cagcttctgc cacttccaca ctccgctctt ctttattctg
     2401 ttcaggcaat tcaacggatt gtggacaggg gtgctgctcg gtatgataag tatcgtcttt
     2461 cttcgtttcg ttatcatgcc tctctggtac aggtacggag ggttggtgac ggagtgaggc
     2521 cggaacggga gttccgcgtt gcgctctgcg ggtattacgg attcgggaat ctcggagacg
     2581 aactgctcgc cgaggcattg atcgcagccc ttgaacggtg cggaatcaag cgcggcgaaa
     2641 tggcgatgct ctccgcctcc ccggaggaat ctgcgcgcgc tcacggggtc tgctctgtct
     2701 cccgctggtc gccgcgcgcc gtatttcgga ttcttcggcg gagcgagacg tttctcctcg
     2761 gcggcggcgg tctgtttcag gacgtcacca gccttcgttc gccgctctat tactggggag
     2821 cgctccggct tgctctcctc gcaggggcgc gcccctggtg cgcagggcag tccgtagggc
     2881 cgttgctgac gaagagagga agactgctgg cgcggtcggc attggggagc tgctcggtcc
     2941 ggggtgtgcg ggatgcccgg tcggcggagc ttctggccga ttggggaatg aactctgttg
     3001 taacgtgcga tccggtcttt tcgctgactc ctccggttac ctctccccgg cacggaagcg
     3061 cgttgcttgt gaacatccgt ccgtggacgg gaggactcgc cagcaaaact gcggaagaat
     3121 ctgctcgcct ggcgtcttcc cgttctctct ccctcacggg gtttgccctt gcggcggagg
     3181 atgccggcat catggaggag cttcgccgca gcggagtttt tccggcggag cgtattgtcc
     3241 tcctcgacgc ggagaactgg aggagcgaat gcggccgtct ttttccggaa gctgatggag
     3301 tggttgcgat gcgcttccac gcgtcgcttc ttgcgctgct cttcggcagg cctctgacgg
     3361 cggtcgcgta cgacccgaag gtggagtctc ttgcctccga atggtcgctt ccttcgtgga
     3421 gcggctccgg ggctcttccg gagcctgcgg cgccgttagg tccgttccgg ccttttgaaa
     3481 agggcgcgga gttcctgaaa actttctccg aaatgtgcgc cgcagcaatg cgacgcgatg
     3541 gaaaggagtg aaggtatgct tcgtgaaccg tccttcctcg cggaaaaagc gtcggagatc
     3601 cggcgcgatg tagtacgtat gatcggtctg gcccgttcgg ggcgtctggc ttcttctctt
     3661 tcgatagtgg atatacttgc ctggctctac tgggacgtcc tctccatcga tccagccgat
     3721 ccgtggtttg agtcccggga cagattcgtg ctcagcaagg ggcgcggagc gcctgcgctt
     3781 tacgcgacgc ttgccaacag gaagtttttc gagcgcgatg agctgtggag ctaccgccgt
     3841 ctgggagcga tgcttcaggg gtacccgcag gcgcttcgca ttcccggcgt cgacgccccc
     3901 ggcggcgggt ctttcggcat gggattggga attgccaacg gactggcgat cgcgctccgg
     3961 cgcgggcggt cgccctgcag ggtcttctgt ctggcaggag aagaggagct gcaagaggga
     4021 gccttctggg aagctgtgat agcggcctcc cgcttctgtc tcgacagtat ctgcctcgtt
     4081 atcgacaggt acaggacgaa ggaagaagct ggaatcccgt ctgcgtcgga cgaggcgttc
     4141 tgcgaaaaga tccgctcctt cggctggacg gcggaagcgt gcgacggaca cgatttttcc
     4201 tctctggggg gggcgttgcg gctcctcggg caa
//
LOCUS       DAOTIL010000128         4233 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminobacteriaceae bacterium isolate AMR_MDS_3026
            NODE_24798_length_4233_cov_3.463858, whole genome shotgun sequence.
ACCESSION   DAOTIL010000128 DAOTIL010000000
VERSION     DAOTIL010000128.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868050
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminobacteriaceae bacterium (wastewater metagenome)
  ORGANISM  Aminobacteriaceae bacterium
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae.
REFERENCE   1  (bases 1 to 4233)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4233)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:19:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,224
            CDSs (total)                      :: 3,198
            Genes (coding)                    :: 3,184
            CDSs (with protein)               :: 3,184
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4233
                     /organism="Aminobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_24798_length_4233_cov_3.463858"
                     /isolate="AMR_MDS_3026"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:3034609"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            13..1299
                     /locus_tag="P5201_03810"
     CDS             13..1299
                     /locus_tag="P5201_03810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013048777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine/sarcosine/betaine reductase component B
                     subunit"
                     /protein_id="HRV97688.1"
                     /translation="MKLELHKVKVNKLAWGDKTFAKGGVLTVNKKELLSVLSGDERLA
                     KVDVDFALPGESVRILPVKDVVEPRFKIEGPGGIFPGMVSDVDSVGEGKTLVLSGAAV
                     VTTGKIVRFQEGIIDMKGPGAEFTPYSQTCNVVVILENVEGIEKHDYETACRMAGLKT
                     AAYLAEKCLGASVDKVETYETPSLKDAMHAHPGLPKVAYLYMLQSQGLLHDTYLYGVD
                     VKKILPTFLNPTEVMDGAIVSGNCVSACDKNNTYVHQNNPIIHNMFKRHGKDFNFLGC
                     IVTNENVTLADKKRSSSYSIKLAKTLGVDGLLISEEGFGNPDADLIMNCWKAERAGIK
                     TTLLTDEYAGQDGASQSLADSCPEGDACVTAGNANEVIVLPPMEKVIGVPEEANVIAG
                     GWQGSLAADGTITVELQAILGSTSELGFTKLGAYTI"
     gene            1356..1832
                     /gene="grdA"
                     /locus_tag="P5201_03815"
     CDS             1356..1832
                     /gene="grdA"
                     /locus_tag="P5201_03815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_133955713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_except=(pos:1485..1487,aa:Sec)
                     /transl_table=11
                     /product="glycine/sarcosine/betaine reductase complex
                     selenoprotein A"
                     /protein_id="HRV97689.1"
                     /translation="MGKLAGKKVLLLGERDGVPGPAMETCLKNSGADIVFSVTECFVU
                     TAAGAMDLQNQQRIKDAVEKYGPENVVVILGSSDAEGAEIYAETVSNGDPTYAGPLAG
                     VQSGLAVFHVFEQEIRDECDANAWEEQISMMEMVLDPEKLSEAVSGIRGQFSKYTL"
     gene            1847..3157
                     /gene="grdB"
                     /locus_tag="P5201_03820"
     CDS             1847..3157
                     /gene="grdB"
                     /locus_tag="P5201_03820"
                     /EC_number="1.21.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006316653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030700 - glycine reductase complex
                     [Evidence IEA];
                     GO_function: GO:0030699 - glycine reductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_except=(pos:2894..2896,aa:Sec)
                     /transl_table=11
                     /product="glycine reductase complex selenoprotein B"
                     /protein_id="HRV97690.1"
                     /translation="MVTYRIIHYINQFYAGIGGEEKADITPEIREGIVGPGQALKAAL
                     GAEAEIVATVICGDSYFASNMEKASEEILGMMSKYKPDAVVAGPAFNAGRYGTACGAL
                     GEAVVKKLGIPVVSGMYPENPGVEMYKKSFYIVETPDSAAGMRKAVPAMAKLVLKLLK
                     GEKLGTPSEEGYIERGIRKNKFYEKLAAERAVDMFTAKLKGEPFVTEYPMPVFDRVAP
                     NPAIKNMKDAVIALVTSGGICPKGNPDHIEASSASKFGEYDISGVMDLTCDSYCTAHG
                     GYDATYADQDADRVLPVDVLRDMEKEGVFKKLHNKFYTTVGNGTAVASAKRFAEEIAK
                     RLIADGVTAVILTSTUGTCTRCGATMVKEIERAGLPVVHICTIVPISLTVGANRIIPA
                     VAIPYPLGDPSRTMAEEKVIRRHLVEKALKALQTEITEQTVFTD"
     gene            3237..>4233
                     /gene="grdC"
                     /locus_tag="P5201_03825"
     CDS             3237..>4233
                     /gene="grdC"
                     /locus_tag="P5201_03825"
                     /EC_number="1.21.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013048772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine/sarcosine/betaine reductase complex
                     component C subunit beta"
                     /protein_id="HRV97691.1"
                     /translation="MATVGIKGYAYCLNHAPETGYHYGNTPHVERETKGETEFLKELP
                     SFLQTYEDARDYAPNQAYIGGISLEKLEQTPQPWYTNRLTGSSRYGAYGEIMPEDEFL
                     GLMDICDVFDIIWLEKEFAASVREKLAVSPVMNEKVLSRLETGHELTEIEGEVAHKKG
                     LALYFGGKVVGCVRNGHDIDDCLFAYVLLENLACKAGGVLSLLHLLKKTGMSPEEVDF
                     VIECSEEGAGDMNQRAGGNFAKAIAEIAGCANASGCDVRSFCAGPVNAMIAGASQVAC
                     GARKNCVVLAGGAVPKLFMNGRDHVKKKMPALEDCMGNFAILLVPDDGELPVMRLD"
BASE COUNT         1021 a         1139 c         1242 g          831 t
ORIGIN      
        1 agacagacag ccatgaaact tgagctgcac aaggtaaaag taaacaaact tgcttggggc
       61 gacaaaacct tcgccaaggg cggagtattg acggtgaaca agaaggagct tctctccgtt
      121 ctttccgggg acgagcgcct tgcgaaagtg gacgttgatt tcgcgcttcc cggcgagagc
      181 gtccgcattc ttccggtaaa agacgtcgtg gagcctcgat tcaagatcga aggcccgggc
      241 ggaatattcc cgggcatggt ctccgatgtc gattccgtgg gcgagggcaa gacgctcgtc
      301 ctctccggag cggccgtagt gaccacgggg aagatcgtcc gtttccagga aggcatcatc
      361 gacatgaagg gacccggagc ggagtttact ccgtattctc agacctgcaa cgtcgtcgtg
      421 atcctggaga acgtcgaggg aatcgaaaag cacgactacg agacggcatg caggatggcc
      481 ggactgaaga ccgccgcgta tcttgccgag aagtgccttg gcgcttcggt cgacaaagtt
      541 gaaacctacg aaacgccctc gctgaaagac gccatgcacg cgcatcccgg gcttccgaag
      601 gtcgcgtatc tgtatatgct ccagtcgcag gggctgctcc acgacacgta tctctacggt
      661 gtggatgtga agaagattct tcccacgttc ctcaacccga ccgaggtcat ggacggcgcc
      721 atcgtctccg gcaactgcgt ttccgcatgc gacaaaaaca acacatacgt gcatcagaac
      781 aacccgatta tccacaacat gttcaaacgc cacggcaaag atttcaactt cctcggctgc
      841 atcgtcacca acgagaacgt caccctcgct gacaaaaaga ggagctcttc ttattcgatc
      901 aagctggcga agacgctcgg cgtggacggg ctgctcattt ccgaggaagg tttcggcaac
      961 cccgacgccg acctcattat gaactgctgg aaggcggagc gagccggaat caagacaacg
     1021 cttctcaccg acgagtacgc cggacaggac ggcgcaagcc agtcgctcgc cgattcctgc
     1081 cccgagggcg acgcgtgcgt gactgccgga aacgccaacg aagtgattgt gcttcctccg
     1141 atggagaaag tcatcggcgt ccccgaagag gccaacgtca tcgccggagg ctggcagggc
     1201 tctctcgccg ccgacgggac gattaccgtc gagcttcagg caatactggg ttccacgagc
     1261 gagcttggtt tcacgaagct gggcgcgtac accatctgat gtgaccaaac cgggaatacc
     1321 cggatacaag tttttcgaca aggaggtgca agagcatggg gaaattggca ggcaaaaaag
     1381 tgcttctcct cggtgaacgc gacggcgttc caggccccgc tatggaaacg tgtctgaaga
     1441 acagcggcgc cgacatcgtc ttctcggtaa ccgaatgctt cgtctgaacc gccgcaggag
     1501 ccatggatct gcagaatcag cagcgcatca aggacgctgt tgaaaagtac ggccctgaaa
     1561 acgtggtcgt cattctcgga tcgtccgatg ccgaaggcgc agaaatctac gccgaaacgg
     1621 tgagcaacgg agatccgacg tatgcaggtc ccttggccgg agtccagtct ggactcgctg
     1681 tatttcatgt ttttgaacag gagattcggg acgagtgcga tgcaaacgcc tgggaagagc
     1741 agatcagcat gatggaaatg gtgcttgatc ccgagaaact gtccgaggcc gtcagcggca
     1801 ttcgcgggca gttcagtaaa tacaccctgt aggaggggag gtcgtcatcg tgacttaccg
     1861 gatcatacac tatataaatc agttttacgc cggaatcggc ggtgaagaga aggccgacat
     1921 aacgccggag attcgcgaag gtatagtcgg ccccggacag gctttaaagg cagctcttgg
     1981 ggccgaagcc gaaatcgtgg ctaccgtcat ctgcggcgac tcgtatttcg cctcgaatat
     2041 ggagaaggcg agcgaggaaa ttcttgggat gatgagcaag tacaaacccg acgcggttgt
     2101 cgccggtcct gcattcaacg ccggacggta cggcactgcg tgcggcgctc tcggcgaagc
     2161 cgtagtgaag aagcttggta tcccggtcgt ctccggtatg tacccggaga acccgggcgt
     2221 cgagatgtac aagaagagtt tttacatcgt ggagactccc gacagcgccg ccggcatgcg
     2281 gaaggctgtt cccgcgatgg caaagcttgt tctgaagctg ctcaagggcg agaagctcgg
     2341 tacgccttcc gaggaaggat acatcgagcg cggcatcaga aagaacaagt tctacgagaa
     2401 gctggctgca gagcgcgcgg ttgatatgtt caccgcaaag ctcaagggag agccgtttgt
     2461 caccgaatat ccgatgcccg tcttcgaccg cgtggccccc aatcccgcga tcaaaaatat
     2521 gaaagacgcg gtcatcgcgc ttgtcacttc cggcggtatc tgccccaaag ggaaccccga
     2581 tcatatcgag gcctccagcg cgagcaaatt cggcgaatac gacatctcgg gcgtgatgga
     2641 tcttacatgc gattcgtatt gcacagccca cggcggttat gacgcaacgt acgccgatca
     2701 ggatgcggac agagtgcttc cggtagacgt tcttcgcgat atggaaaaag agggcgtctt
     2761 caagaagctg cataacaagt tctatactac ggtcggcaac ggtactgccg tggccagtgc
     2821 caaacgcttc gccgaagaaa tcgcgaaaag gctgatcgcg gacggagtga ccgccgttat
     2881 cctcacatcc acctgaggca cctgcactcg ttgcggtgca acgatggtca aagaaatcga
     2941 acgcgcaggt cttcctgtgg tgcacatctg caccatagtc cccatctccc tcacggtagg
     3001 agcaaacaga atcatccccg cagttgctat accctatcct ctcggcgacc catcgaggac
     3061 aatggcggaa gaaaaagtaa tccgtcgcca tcttgttgaa aaagccttga aggcacttca
     3121 aacggagata acggaacaga cggtatttac cgactgattc tgttgcgtat acggccggaa
     3181 ccggggggaa ccccggaacc ggccgtgcga gaaaagcaca aggaggttgg aacgtcatgg
     3241 ccaccgtagg aatcaaagga tatgcctact gcctgaatca cgctcccgaa acgggatatc
     3301 attacggaaa cacgccgcac gttgagcgag agacgaaggg agagacggag tttctcaagg
     3361 aactgccctc ttttttacag acgtatgaag atgccaggga ttatgcgccc aaccaggcat
     3421 acatcggcgg tatctctctt gaaaagctgg aacagactcc ccagccctgg tacacaaaca
     3481 ggctgaccgg ctcgagccgg tacggcgctt atggtgaaat tatgcctgag gacgagttcc
     3541 tcggtctcat ggatatatgc gacgtcttcg atatcatttg gctcgaaaag gaatttgcgg
     3601 cttcggttcg ggagaagctg gccgtaagcc cggtgatgaa cgaaaaggtg ctctcccgcc
     3661 tcgagaccgg acacgagctt acagagattg agggggaggt tgctcataaa aaaggacttg
     3721 ccctctattt cggaggcaag gttgtcggat gtgtccggaa cggccatgac atagacgact
     3781 gtctcttcgc ctatgtgctt cttgagaatc ttgcctgcaa agcgggaggc gtgctctccc
     3841 tgcttcatct gctcaagaag accgggatgt ctcccgaaga agttgatttt gtcattgaat
     3901 gttccgagga gggcgccgga gatatgaatc agagggccgg aggcaacttc gccaaggcga
     3961 tcgcggaaat cgccggatgt gcgaacgcta gcggttgcga cgtgcggagc ttctgtgccg
     4021 gtcctgtgaa tgccatgatt gccggcgctt cccaagttgc ctgcggcgcc agaaagaact
     4081 gcgttgtact tgccggcgga gccgttccga agctcttcat gaatgggcgc gaccatgtga
     4141 agaagaagat gcccgcgctt gaggactgca tgggcaattt tgcgatactc cttgtcccgg
     4201 atgacggaga acttccggtg atgcgtctcg acg
//
LOCUS       DAOTIL010000129         4229 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminobacteriaceae bacterium isolate AMR_MDS_3026
            NODE_24830_length_4229_cov_3.890034, whole genome shotgun sequence.
ACCESSION   DAOTIL010000129 DAOTIL010000000
VERSION     DAOTIL010000129.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868050
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminobacteriaceae bacterium (wastewater metagenome)
  ORGANISM  Aminobacteriaceae bacterium
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae.
REFERENCE   1  (bases 1 to 4229)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4229)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:19:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,224
            CDSs (total)                      :: 3,198
            Genes (coding)                    :: 3,184
            CDSs (with protein)               :: 3,184
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4229
                     /organism="Aminobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_24830_length_4229_cov_3.890034"
                     /isolate="AMR_MDS_3026"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:3034609"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..266)
                     /locus_tag="P5201_03830"
     CDS             complement(<1..266)
                     /locus_tag="P5201_03830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013047429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate kinase"
                     /protein_id="HRV97692.1"
                     /translation="MIVQKYGGSSVATPEKIKNVAARIKKRVDEGEKLIVVVSAMGKT
                     TNSLISLAQEVSAEPSPRELDMLLATGEQMTSALLAMALSDFGIP"
     gene            complement(301..1002)
                     /gene="dapD"
                     /locus_tag="P5201_03835"
     CDS             complement(301..1002)
                     /gene="dapD"
                     /locus_tag="P5201_03835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008522327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047200 - tetrahydrodipicolinate
                     N-acetyltransferase activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2,3,4,5-tetrahydropyridine-2,6-dicarboxylate
                     N-acetyltransferase"
                     /protein_id="HRV97693.1"
                     /translation="MRTEEIIRLIKESKKKTPAKAYIAGDLEGLDGERITFIGGKDFG
                     ILLGDRDDVERVLVENAGKIRSAHVEISARNSAVPLADLTKYEARIEPGAIIRDMVEV
                     RKGAVVMMGAVINIGAVIGERTMIDMNVVVGGRAEIGADCHIGAGAVVAGVIEPPSAT
                     PVVIEDNVLVGANAVILEGVRVGKGAVVAAGAVVTKDVPPHSVVAGSPAKVIKEVDAK
                     TESKTQIVQDLRTLK"
     gene            complement(984..1634)
                     /locus_tag="P5201_03840"
     CDS             complement(984..1634)
                     /locus_tag="P5201_03840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009201093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008839 - 4-hydroxy-tetrahydrodipicolinate
                     reductase [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrodipicolinate reductase C-terminal
                     domain-containing protein"
                     /protein_id="HRV97694.1"
                     /translation="MRYGLVGASGRMGGEIRKAFAAHELTFSLDVGGETHDAAPQVLV
                     DFSLPAAFPSTFAVARRFVCPLVIGTTGLSAEQLAELRTLGEQVAVVQSFNFATGVTL
                     LKMMLREFAPLFADWDVEISETHHNKKKDAPSGTAILLREATGRDCPTHSLRLGGIPG
                     DHTVCYANEGEVLTLTHRALSRSVFALGALKAAEFAVSAAPGFYSFEEVLSCGLKR"
     gene            complement(1631..2515)
                     /gene="dapA"
                     /locus_tag="P5201_03845"
     CDS             complement(1631..2515)
                     /gene="dapA"
                     /locus_tag="P5201_03845"
                     /EC_number="4.3.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013047426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008840 - 4-hydroxy-tetrahydrodipicolinate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate synthase"
                     /protein_id="HRV97695.1"
                     /translation="MFGGTGTAVVTPFKDGSVDYESFERFLNWQIEGGVEFLVVLGTT
                     GESPTITADERAEIIKFAVKAAAGRVRVVIGTGTNSTASTILLSQQAEGLGADGLLVV
                     GPYYNKPTQEGFYQHFKAVAESVKTPIIVYNVPGRTGSNIAPETVYRLSKLRMIAGIK
                     EAAGDMAQIDMLVRLIKKERPDFAILSGNDDQAFHLVNSGGDGVISVLSNVMPRETSD
                     MIRLALAGEVEKARALHHRMFPLMKNLFVEANPIPVKYAASRLGFCRNELRLPLVPAS
                     EKCVAIVDASMKECGTAL"
     gene            3038..3787
                     /locus_tag="P5201_03850"
     CDS             3038..3787
                     /locus_tag="P5201_03850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005660286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HRV97696.1"
                     /translation="MKLLKIALVLVFCLCSGSAWALTVDDITYMTEDYPPFNMAGADG
                     VATGAAVDTLTTIFERMGAAKTAKDIQVLPWARGYREVQSTPNTCLFLMTLTDERKPL
                     FKWVGPVVVNTFDAIALKSKGMKIASAEELVNLKAGVIRDDVGDALAQKAGIKQIERV
                     PSNDQNIKKLNEGRIDIWVFGESSAKLQVKEMGLNPDDYESVWRMSESGLYFAFHKDV
                     DDALIESMQKVLDEMKADGAYEAIMKKYNVQ"
     gene            4008..>4229
                     /locus_tag="P5201_03855"
     CDS             4008..>4229
                     /locus_tag="P5201_03855"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HRV97697.1"
                     /translation="MKLMKVFLVMAFCLFSVSAWALTVDDVTFMTEDYPPFNMAGADG
                     TATGASVEVLVGIFEKLGAAKTVKDIQVLP"
BASE COUNT          856 a         1308 c         1170 g          895 t
ORIGIN      
        1 ggaattccaa agtcgctcaa cgccatcgcc agaagggcgg aggtcatctg ctctcccgtt
       61 gcgaggagca tgtccagctc gcggggcgac ggctccgccg acacctcctg cgcgagggag
      121 atgagcgaat tcgtggtctt tcccatcgcg gagactacca cgatgagctt ctccccctcg
      181 tccacgcgct tttttatcct ggcggcgacg ttctttatct tctcgggcgt ggccaccgac
      241 gacccgccgt acttctgcac gatcacttcc gcttctctcc ccttcgtccg accggttcga
      301 tcacttgagc gtccggaggt cctgaacgat ctgcgtcttc gactccgtct tggcgtccac
      361 ttccttgatc accttcgcgg gcgatccggc gaccacggaa tgcggcggca cgtccttcgt
      421 cacgacggct ccggccgcca caacggcgcc ctttccgacc cgcactcctt ccagaatgac
      481 cgcgttcgcg ccgacgagca cgttatcttc gataaccacg ggcgttgcgc tcgggggctc
      541 gatcactccg gcaaccaccg cgcctgcgcc gatgtggcag tccgcgccga tctccgcccg
      601 tcctccgacc acgacgttca tatcgatcat ggtccgttcg ccgatgaccg cgccgatgtt
      661 gatcaccgcg cccatcatca ccacagcgcc tttgcggacc tccaccatgt cgcggataat
      721 cgcgccgggc tcgatgcgcg cctcgtactt cgtcaggtcg gcgaggggca cggccgagtt
      781 gcgggccgaa atctcaacgt gcgcggagcg aatcttcccc gcgttctcca cgagcacccg
      841 ctccacgtcg tcacggtcgc cgagcagaat gccgaagtcc tttcctccga tgaaggtgat
      901 ccgctcgccg tcaagtccct caagatcccc ggcgatgtac gccttggcgg gcgttttctt
      961 cttcgactcc ttgatgagcc gtattatctc ttcagtccgc atgagaggac ctcctcgaaa
     1021 gaatagaatc cgggcgcggc cgagaccgca aactctgcgg ccttcagcgc gccgagggcg
     1081 aagacgctcc gcgaaagagc ccggtgcgtc agggtaagga cctcgccctc gttcgcgtag
     1141 cagaccgtat ggtctccggg aatccccccg aggcgcagcg aatgcgtcgg acagtcgcgc
     1201 cccgtcgcct cccggaggag aatcgccgtc cccgaagggg cgtccttctt cttattgtgg
     1261 tgcgtctcgc tgatctcgac gtcccagtcc gcgaagagcg gggcgaactc gcggagcatc
     1321 atcttcagca acgtgacgcc ggtcgcgaag ttgaagctct gcaccaccgc gacctgctcc
     1381 ccgagcgttc tcagctcggc gagctgctcc gcggagagac cggtcgtccc gatcaccagc
     1441 gggcagacaa agcgccgcgc aaccgcgaac gttgagggga aggccgccgg aagggagaag
     1501 tcgaccagca cctgcggcgc ggcgtcgtgc gtttcaccgc cgacgtcgag ggaaaaagta
     1561 agttcgtgcg cggcgaaggc cttcctgatc tcgccgccca tcctccccga agcgcctaca
     1621 agaccgtatc tcatagcgcc gttccgcact ccttcatgga ggcgtcgacg atcgcaacgc
     1681 acttctccga cgccggaacg agcggcaggc gaagttcgtt gcggcagaag ccgagcctgc
     1741 tggccgcgta cttcacgggg atcggattcg cctcgacgaa gagattcttc atgagcggga
     1801 acatcctgtg atgcagcgca cgcgccttct ccacctctcc tgcaagagcc aggcggatca
     1861 tgtcggacgt ctcgcgcggc atcacgttgg agagcactga gattacgccg tcgccgccgc
     1921 tgttcacaag gtggaacgcc tgatcgtcgt tccccgaaag gatggcgaaa tcgggacgtt
     1981 ccttcttgat gaggcgcacg agcatgtcga tctgcgccat atcccccgcc gcttctttga
     2041 tccccgcgat catgcggagc ttgctcaggc ggtacaccgt ctccggcgcg atgttcgacc
     2101 cggtccgccc gggcacgttg tagacgatga tcggggtttt cacgctctca gcgaccgcct
     2161 tgaagtgctg gtagaaaccc tcctgcgtcg gtttgttgta gtacggcccc acgaccagca
     2221 gaccgtcggc gcccaacccc tccgcctgct gcgacagcag aatcgtgctc gccgtcgaat
     2281 tcgtccccgt gccgataacc acgcgcactc tgcccgccgc cgccttcacg gcgaacttga
     2341 taatctccgc ccgttcgtcg gcggttatag tcggactttc gcctgtggtc ccaaggacga
     2401 cgaggaactc gacgcctcct tcgatctgcc agttcaaaaa ccgctcgaaa ctctcgtaat
     2461 ccacgctccc gtccttgaac ggggttacca ccgcagtacc tgttcctcca aacatcgctc
     2521 gcacctcctg atcatagtgt cggttcccta tacaaaaagg cccgtccccg aaagggacga
     2581 gcctgaactc gcggtaccac cctgattgga catgtcgaaa tatgtcccgc tcggaccctg
     2641 tcacggaggt ccggaccgtg agtgtactcc ggcgaacgtc tctttgcgtc cgtctttcca
     2701 ctccagctca ccgggctttg ggccttcttc tccgccgccg gaacgtcttt cagccgatga
     2761 acgttcctct ctgtacgcga cgctttttaa aaaaaggcga tccggttcat cgctttttca
     2821 aatctaacga tatgttgagt gattttaggc aaagaacgcc gatttgtcaa ccatgaattt
     2881 tcaactggtg aaaccgggct tgacttttgg agcaaatttg atatattcca atcaacgcat
     2941 tgatggtttc tttagacgaa gaaaaaggaa ttggtccgta ccattacccc atgtatcaga
     3001 atcaccataa ttccataatt ttgggagggg atacgtcgtg aagttgttga agattgctct
     3061 tgtactggtc ttttgtcttt gttcagggag cgcatgggct cttacagtgg atgatattac
     3121 gtatatgacc gaggattatc ctccgttcaa catggcggga gcggacggag tggcaacggg
     3181 cgcggcagtg gataccttga cgaccatctt cgagagaatg ggcgcggcca agacggcgaa
     3241 ggacattcag gttcttccct gggcccgggg ataccgggaa gttcagagca cgccgaacac
     3301 ctgcctcttc ctgatgactc tcacggacga gcgcaaaccc ctgttcaagt gggtcggtcc
     3361 ggtcgtcgtc aacacgttcg acgcaatcgc cctcaagagc aagggaatga agatcgcctc
     3421 cgccgaggag cttgtcaacc tcaaggccgg agtcatccgt gacgatgtcg gcgacgcgct
     3481 cgctcagaaa gccggtatca agcagataga aagagtgcct tccaacgacc agaatatcaa
     3541 gaagctgaac gaaggccgca tcgacatctg ggtcttcgga gaaagcagcg cgaagctcca
     3601 ggtcaaggag atggggctga atccggacga ctacgaaagc gtgtggcgta tgagcgagag
     3661 cggcctctat tttgccttcc ataaggacgt cgacgatgcg cttatcgagt cgatgcagaa
     3721 ggtcctcgac gagatgaagg ccgacggcgc gtacgaagca atcatgaaga agtacaacgt
     3781 acaatagcaa aaaacgcttt ctaaattgta cctgacaagg ccgcctcaat ccgggcggcc
     3841 ttgtttcgtt ttcttttcac ccacgccaca gataaaaaac cttgactttt ttagcaattt
     3901 tgatatattt tatttatacc actcaatcac tcttttttct atactgctcc ggatcgaatg
     3961 aatcaatcga aaccggcgcc ccaaacacca aaaggagggg aaacatagtg aaattgatga
     4021 aggtttttct cgtaatggct ttttgcttgt tctcggtaag cgcgtgggcg ctgacggtgg
     4081 atgatgttac gttcatgacc gaggattatc ctccgttcaa catggcggga gcggacggaa
     4141 cggcgacagg agcgtccgtt gaggtactcg tcggcatctt tgagaaactg ggggcggcga
     4201 agactgtaaa ggatatccag gtgctcccg
//
LOCUS       DAOTIL010000130         4225 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminobacteriaceae bacterium isolate AMR_MDS_3026
            NODE_24864_length_4225_cov_3.977938, whole genome shotgun sequence.
ACCESSION   DAOTIL010000130 DAOTIL010000000
VERSION     DAOTIL010000130.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868050
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminobacteriaceae bacterium (wastewater metagenome)
  ORGANISM  Aminobacteriaceae bacterium
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae.
REFERENCE   1  (bases 1 to 4225)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4225)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:19:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,224
            CDSs (total)                      :: 3,198
            Genes (coding)                    :: 3,184
            CDSs (with protein)               :: 3,184
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4225
                     /organism="Aminobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_24864_length_4225_cov_3.977938"
                     /isolate="AMR_MDS_3026"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:3034609"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..524)
                     /locus_tag="P5201_03860"
     CDS             complement(<1..524)
                     /locus_tag="P5201_03860"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRV97698.1"
                     /translation="MGDLFPGIKKIQYEGPLSQNPLAFKEYNADEVVAGKPMKEHFRF
                     AMSWWHTICSSGSDMFGSGTAIRPWDYEPHPVKRAKLAADGAFEFMEKLGLEYFCFHD
                     RDIAPEALTLAETNERLDEVCDYVKGLMSKTGIKLLWGTANLFSHPRYMHGAATSCNA
                     DVFAYAAAQVKKAMT"
     gene            complement(609..1802)
                     /locus_tag="P5201_03865"
     CDS             complement(609..1802)
                     /locus_tag="P5201_03865"
                     /inference="COORDINATES: protein motif:HMM:NF012692.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family transcriptional regulator"
                     /protein_id="HRV97699.1"
                     /translation="MRIRSINAEKMGLSNRLTLLNLIRTTPGVSRRELARLAGLDPST
                     VTKIITSLLEKGLVIEHGVRDADHPGRKSIMLRVYRDAAVALIAEIGVEKTRIAKGFL
                     DASFEVLDEFDTPPKEDEFFSVFAGSLRKKAFSFPHTAYCALSVAVPGMVEWGSNVLR
                     SLPHMGWKNVDFEARLGKLLPEMELTVFTANEAKLSLIAEMFRNGGVSGLHSGVYVYL
                     SQGVGGAILMGDKIIHGLSNTAGEIGHMTVDPSGPLCHCGNRGCFETFVSIDTVVRNF
                     ENLGASLPGSDFREKFRALLRLFQEGNDAARRVLEEMGDFLATGIANLVSILNPDFIM
                     LGGMGSILPPSFVAGVGENVAKKTPGATSPDFLLTTASLGIESAALEGATLMAMNHYV
                     KKAVK"
     gene            complement(1927..2664)
                     /locus_tag="P5201_03870"
     CDS             complement(1927..2664)
                     /locus_tag="P5201_03870"
                     /inference="COORDINATES: protein motif:HMM:NF013782.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016790 - thiolester hydrolase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioesterase"
                     /protein_id="HRV97700.1"
                     /translation="MFEAEYSIPYAGIGPDGAMKLPVLLDILQDMADRDAHRMSMTVA
                     DLLPRGLSWVLRQYRVVVDRYPGHESMLKVRTWHGPRRNLHSIRAFEVRDAAGPVASA
                     WTSWILIDTLRGRPLRLDRHTTEVYTREAYPMDGDAPSLPVVEDFAYDTFFSVRRWDL
                     DRNAHVNNAVYFSWAVESAPDAVAAEYELVKVEAEYLKPVEKNGDVRVRTALLPADQG
                     EKAFFHLIEGPDGEVKAHFATRWRGRK"
     gene            2855..3916
                     /locus_tag="P5201_03875"
     CDS             2855..3916
                     /locus_tag="P5201_03875"
                     /inference="COORDINATES: protein motif:HMM:NF013700.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HRV97701.1"
                     /translation="MRRILFLTLLLVITAAPSVIAEQPPFGVYTVKAGDTVDSVAGRY
                     GVDPANLASANGIAANDGLPPEGTVLLVPKSPGDVLATLYEAKRRGLGAWPKPKYADE
                     FLPPLTEEPPSDKKPSAPKERPQKAEHTPAPAVSSQQPAAPADGTVRTHKVQEGETMY
                     GISRLYGLSLSALLQANNLTESSIIKIGETLTIPGTSAEKPEPHVQRPTPPQNEPIVE
                     KTPSKAPLSPTTPMRLTWPLRGGPPPTHTGTSFGEGLSAKAAPGEPVHAAADATVLHG
                     GWMRNFGNAIYLNHGNGIATFYGGLGIIYVKSGEKIRRGTKIALVGEIQGAEPRFVFH
                     VLKEGKSVDPRPWIEGAKQ"
BASE COUNT          938 a         1258 c         1118 g          911 t
ORIGIN      
        1 gtcatggcct ttttgacttg ggcggcggca taggcaaaga cgtccgcgtt gcacgatgtg
       61 gcggcgccgt gcatgtatcg cgggtgacta aagaggttcg cagtccccca aagtagcttg
      121 attcccgtct tgctcatcag ccccttgaca tagtcgcaaa cttcgtcgag gcgctcgttc
      181 gtctcggcaa gcgtcaacgc ctcgggagcg atatccctgt cgtgaaaaca gaaatactcg
      241 agccccagct tctccatgaa ctcgaaagcg ccgtccgccg cgagcttcgc ccgcttgacc
      301 gggtgcggct cgtaatccca cggacggata gcggttcccg agccgaacat atcgcttccc
      361 gaagagcata ttgtgtgcca ccatgacatg gcgaagcgga agtgttcctt cataggcttt
      421 ccggcgacga cttcgtcggc gttgtactcc ttgaaagcga gcggattttg tgaaagcggc
      481 ccctcatatt gaatcttctt tattccgggg aagagatctc ccattgtatt tacctccttt
      541 tcagagtgca gtttcttttt ccttgacgcc ctcacacgag aacatctcct tggattcgtc
      601 gcctttcttc atttcactgc tttcttcaca taatggttca tcgccatgag cgtcgctcct
      661 tcaagagcgg cggattctat acccaatgaa gcggtggtga gcaagaaatc cggggaagtc
      721 gcgccgggcg ttttttttgc gacgttttct ccgacacccg ctacaaacga ggggggcaga
      781 atggatccca ttcctccaag catgatgaag tcgggattaa ggatactgac aaggttggcg
      841 atacctgttg cgagaaagtc tcccatctcc tcgagaacac ggcgcgcagc gtcgttccct
      901 tcctggaaca gcctgagaag cgctctgaac ttttctcgaa aatcgcttcc cggcaaggag
      961 gctcccaggt tctcgaagtt cctcacaaca gtgtctatgg aaacgaacgt ctcgaaacac
     1021 cctcggttgc cgcagtggca gagagggcct gaaggatcga ccgtcatatg tccgatttca
     1081 cccgccgtat tgctgagtcc atggatgatc ttatccccca tgaggatggc gcctcccacc
     1141 ccctgcgaaa gatagacata gacgccgctg tgcagcccgg agactccgcc gttccggaac
     1201 atctcggcga tcagggagag tttggcctcg tttgcggtga acacagtgag ctccatttcg
     1261 ggaagcaact tcccgagacg cgcttcaaaa tcgacgtttt tccaccccat atgcggaaga
     1321 gagcgcagca cgttgctccc ccactccacc attcccggaa cggcgacgct cagcgcacaa
     1381 tacgccgtat gagggaagga gaacgctttc tttcgcaggc tcccggcgaa aacagagaaa
     1441 aattcgtcct ccttcggcgg cgtgtcaaat tcgtcaagca cttcaaaaga agcgtcgaga
     1501 aaccctttcg cgatccgggt tttctcgacg ccgatctctg caatcagcgc aacggcagca
     1561 tcacggtata cgcggagcat gatggacttt ctccccggat ggtcggcgtc gcgcactcca
     1621 tgttcaatga cgagcccttt ttcaaggagc gaggtgataa tcttggtcac tgtgcttgga
     1681 tcaagcccgg cgaggcgggc aagttcccgc ctgctcacac cgggagtggt tcggataagg
     1741 ttgagcaagg tcagtctgtt cgaaagaccc attttttcag cgttgatgct ccggattctc
     1801 atcccgtccc tcctctgcgt cgaatcaata cggcggctcg cggtacggag attagtttat
     1861 tgatgaaaca aaccaattct agagagaaag atgagagttg tcaatggcga attttctttc
     1921 agaagtttat ttccggcctc tccaccgtgt ggcgaaatgc gccttcactt ccccgtccgg
     1981 cccttcgatt aagtgaaaga aagccttttc gccttgatcg gccggaagca acgcggtcct
     2041 gacgcgcacg tcgccgtttt tctccacggg tttcaggtat tcggcctcca cttttaccag
     2101 ctcgtactcc gcggccactg catccggcgc agattcgaca gcccaagaga agtagacggc
     2161 gttgttcaca tgggcgtttc ggtcaagatc ccatctgcgg acggagaaaa atgtatcgta
     2221 ggcaaagtcc tcaactacag gaagagaagg agcgtctccg tccatcgggt atgcctctct
     2281 cgtgtagacc tccgtggtgt gccggtcaag cctgagggga cgtccgcgca gagtatcgat
     2341 aaggatccag ctcgtccatg cggacgcaac aggtccagct gcgtcgcgaa cctcgaaagc
     2401 gcggatcgaa tgcagatttc tccgcggtcc gtgccacgtc ctgaccttca acatactctc
     2461 gtgcccggga tagcggtcca cgacaaccct gtactggcgc agcacccagc tcaaccctct
     2521 cggcagaaga tcggccacgg tcatggacat cctgtgcgca tcccggtcgg ccatgtcctg
     2581 caatatatcg agaaggacgg gaagcttcat ggccccgtcc ggtccaatcc ccgcgtaggg
     2641 tatggagtat tcggcttcaa acacgtaatc atctcgtttc tctggaatat aatatgcgaa
     2701 gggaacagga cggaacgtgc tgcatctttt ccccagcgtc gctttttcga ggtatattat
     2761 agtacagaac gagtttcttc tctcccgtgc ggaagaacag gctcccgccg ggcgaacatc
     2821 atgtccttcg ggacgccagg aggttgaaca gagcatgaga cgcatactct tcctcacact
     2881 tcttctcgtt attacggccg cgccttccgt tatcgcggaa cagcccccgt tcggcgtcta
     2941 cacggtaaaa gccggagaca ccgtagactc ggtagccggg aggtacggcg tcgaccctgc
     3001 gaacctcgcc tcagccaacg ggatagctgc gaacgatggt cttccaccgg aggggacggt
     3061 gctgctggtt cccaaatcac cgggcgacgt tcttgccacc ctctacgagg cgaaacgccg
     3121 cggcctggga gcatggccga agccgaaata cgccgatgag tttctgccgc ccttgacaga
     3181 agaaccgccc tcggacaaga agccctcggc cccgaaggag cgcccgcaga aagccgaaca
     3241 cacacccgct ccggcagtat cttctcaaca gcctgcggct ccggcggacg gaacggtacg
     3301 cacccataaa gtacaggagg gagagaccat gtacggaatt tcgcgtctct acggcctctc
     3361 gctgtccgcg ctgctgcaag ccaacaatct taccgaaagt tccattatca agataggcga
     3421 gacgctcacg attcccggaa cctcggccga aaagccggaa cctcatgtgc agcgcccgac
     3481 cccgccgcag aacgagccga tcgtcgaaaa gacgccttct aaagcgcctc tatcacctac
     3541 cacaccgatg cgcctgacct ggcctctccg tggaggtcct ccccccacgc ataccggaac
     3601 gagcttcgga gaagggcttt ccgcaaaggc tgcccccggc gaaccggttc atgcggcggc
     3661 ggatgcgaca gtccttcacg gcggatggat gcgtaatttc ggcaacgcga tctatctgaa
     3721 ccacggcaat ggtattgcca ctttttacgg aggactcggg atcatctacg taaaatccgg
     3781 agagaagata cggcgtggaa caaagatcgc ccttgtcgga gagattcagg gagccgagcc
     3841 gaggtttgtc ttccatgttt taaaagaagg gaaatccgtc gatcccagac cctggataga
     3901 aggcgcaaaa cagtaaccgt ttttcagacg cggacctcac agcgcggggg agagtgtcgc
     3961 cggacgactt ccccccgacg ctgcaagtcc gccctccgct cagtaacgaa gtagctgctc
     4021 cataagaacc gccctcggtg tcattcattt cctcaagtca tctggacaaa aagcgagagt
     4081 aataacacat cctccggcga aaaatacccg tgaatccaca gagaaaaagt gagcagctca
     4141 aatactccgc tgcattctct tctttcggca tgattggctt ttgccccgat gctttccccc
     4201 ggcactgaag atcgtgtgct ctttt
//
LOCUS       DAOTIL010000131         4208 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminobacteriaceae bacterium isolate AMR_MDS_3026
            NODE_24995_length_4208_cov_3.858175, whole genome shotgun sequence.
ACCESSION   DAOTIL010000131 DAOTIL010000000
VERSION     DAOTIL010000131.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868050
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminobacteriaceae bacterium (wastewater metagenome)
  ORGANISM  Aminobacteriaceae bacterium
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae.
REFERENCE   1  (bases 1 to 4208)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4208)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:19:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,224
            CDSs (total)                      :: 3,198
            Genes (coding)                    :: 3,184
            CDSs (with protein)               :: 3,184
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4208
                     /organism="Aminobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_24995_length_4208_cov_3.858175"
                     /isolate="AMR_MDS_3026"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:3034609"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1029
                     /locus_tag="P5201_03880"
     CDS             <1..1029
                     /locus_tag="P5201_03880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008521374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--glyoxylate aminotransferase family
                     protein"
                     /protein_id="HRV97702.1"
                     /translation="IADLKAMWRCDGEVFVVAGSGTMGMEMAIANTTKRGDNVLICSN
                     GYFGDRFIDMCTRKGLNTDVLAAEWGSSVTPESVEKALASKQYQVVTVTHVETSTGAE
                     APIAAIGEVMKKHPEIIYIVDGVAASGGAEQYMDSMGIDVVLSCTQKAFGVAPGLTMV
                     WASKKAMEKRATLGAIPESYIDFDKWLPVMHDPSKYWGTPAINLVWALKESVRIMKEE
                     GLEERYARHRRQAAVFDAALEAIGFKVAAQKAFRAPTLSVYLYPEGSGIDDMKFRTVL
                     AAEGAQTAGCLGGFAGKGFRMGHMGNIDKHTLVSAIAAVERSCVKCGYSIELGKALGV
                     LQKGLLEE"
     gene            complement(1121..2833)
                     /locus_tag="P5201_03885"
     CDS             complement(1121..2833)
                     /locus_tag="P5201_03885"
                     /inference="COORDINATES: protein motif:HMM:NF015983.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HRV97703.1"
                     /translation="MKILLCNVSKKYETTAHDYVGLFYLAGALEAAGFEPLVFHGKAE
                     GLLDEIARHRPEAVGFSCDFDNVRIIEDVSQKIKERWGIPVVVGGPQSIALGRTFLES
                     SGADYILRGEGEISLPLLLNAVLKGEGELDEVKGLAMIKDGVFRETRPEIIDDPDALP
                     LPAYHTSLHSDRAYGNLIFTGRGCPFQCAYCAPSPGKRKVRLRNIPLVFEEIKTNLRN
                     NPGLRCLIVMDDTFTLGRERIEAFCEGMRELRKELDLVWYCECHVGRIKSFMDVLPVM
                     IDAGLIRLQIGIESGDQNVIDQYNKHVRIDDILEFVSYAADCGLAQIATNFIVGGPKE
                     REGATAELIRELIDRAPGVIDIITGFLRAYPGTEIFENPEKFGLQLHDESGRRTGDDY
                     PFVTPFGESQDQVMRRRQELNKVIRRSMQQKIKNHELKQKTVMKQFEAALKYRIKSRW
                     FGEIADNPRAYEYYSLLYANEGEAFEESMDFSKDYPLRTFELWRAMTFSDGFPRLDGM
                     ALSPMEYELLLRCAGKRSLSTIQTELYELFGGSYEDEAEFRRHFVDMLRRCDDKYWLT
                     HFVI"
     gene            complement(2846..>4208)
                     /locus_tag="P5201_03890"
     CDS             complement(2846..>4208)
                     /locus_tag="P5201_03890"
                     /inference="COORDINATES: protein motif:HMM:NF015983.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HRV97704.1"
                     /translation="TKKSLEDIPGVFFIDENGECVDRGEGPVVENLDELPFPAYEKSM
                     TPVDYSNLYIMTSRGCPYSCVFCHEGALKRPVRRRSVANVVAEMRHLLLKYPELKYFL
                     FCDDTLVANPEWLDEFCREVKALQAVRPFQFYCEADAASLSKRPEVIKDMVAAGLNRL
                     QIGIESADREMLRIYRKNLAPEMVETVVKAAYDAGVQQVFGALLVGGPFENREHIEKN
                     KAFAARLMRLGPGMMEIAPSILMPYPMTDIGRHPEKYGLKIYDRQGVASISDYPLMES
                     ASMDRREIMAAYQEYIQSFVNEVKAMLDTGELGHEDILRAFRTSMNSKGPKYWSNIIA
                     HHKPMLAPYYTMLAREAASRIGDVQPGELPKWRPQRLLEMWRDVDFSRGYPLARGEAL
                     SPFEYEVLKYSTGKSTIAEIAGILYERFGKPFEETTEDLTGRIVETLKAFDKKYWLLV
                     VPY"
BASE COUNT          805 a         1260 c         1234 g          909 t
ORIGIN      
        1 atcgccgatc tgaaggctat gtggcgctgc gacggagagg tcttcgtcgt tgccggttcc
       61 ggcacaatgg ggatggagat ggccatcgcg aacacgacga agcgaggcga caacgtcctc
      121 atctgctcca acggctattt cggcgaccgc ttcatcgata tgtgtacccg caaggggctg
      181 aacaccgatg ttctcgccgc ggagtgggga tcttccgtca cgccggaatc ggtggagaag
      241 gcgcttgcct cgaagcagta ccaggtggtc acagtgacgc acgtggaaac ctccacagga
      301 gcggaagctc ccatcgccgc catcggcgag gtgatgaaga agcatcccga gatcatctac
      361 atcgtggacg gcgtggcggc ctccggcggc gcggagcagt acatggacag catggggatc
      421 gacgtcgtgc tctcctgtac gcagaaggcg ttcggcgtgg ccccgggcct caccatggtc
      481 tgggcgagca agaaggcgat ggagaagcgc gcgacgctcg gcgcgattcc cgagtcgtac
      541 atcgacttcg acaagtggct tcccgtcatg cacgacccct cgaagtactg gggcaccccc
      601 gccatcaacc tcgtctgggc gctgaaggag tccgtgcgca tcatgaagga ggaggggctg
      661 gaggagcggt atgcccggca ccgccgccag gctgcggtgt tcgacgccgc gctggaggcc
      721 atcggcttca aagtcgccgc gcagaaggcc ttccgcgcgc ctactctttc ggtctacctc
      781 taccctgaag gttcggggat cgacgacatg aagttccgca cagtcctcgc cgccgagggc
      841 gcccagaccg caggatgcct cggcggattc gccggaaagg gtttccgcat ggggcacatg
      901 gggaacatcg acaagcacac gctcgtctcg gccatcgccg ccgtggagcg ctcatgcgtg
      961 aagtgcggct attcgatcga actcggcaag gccctcggcg tgttgcagaa agggctcttg
     1021 gaggagtaac agctcccttt ttacacgcga ataccccgcc ttccggagct ctccggaggg
     1081 cggggtatca accagcaacg ctccggactc ccggtcgagg ttagatcacg aagtgcgtca
     1141 gccagtattt atcgtcgcac cggcgcagca tgtcgacgaa gtgacgacgg aattcagcct
     1201 catcttcata cgagccgccg aagagttcgt agagttccgt ctgaatcgtg ctcagacttc
     1261 ttttgcccgc acaccgcagg agaagttcgt actccatcgg cgagagcgcc atgccgtcaa
     1321 gccgggggaa accgtccgaa aacgtcatgg cgcgccataa ttcgaaggtc ctcagggggt
     1381 aatccttgga gaaatccatg gactcttcga acgcctctcc ctcgtttgca taaagcaaag
     1441 agtagtattc ataggctcgc gggttgtccg cgatttcccc gaaccaccgg ctctttatcc
     1501 tgtatttcag cgccgcttca aactgtttca tcaccgtttt ctgtttcagt tcatgattct
     1561 tgattttctg ctgcatgctg cggcggatca ccttgttgag ttcctggcgc cggcgcatca
     1621 cctgatcctg gctctctcca aacggcgtca cgaaggggta gtcgtccccc gtgcggcggc
     1681 cggattcgtc gtggagctgg agcccgaatt tttccgggtt ctcgaagatc tccgttcccg
     1741 gataggcccg cagaaaaccg gtgatgatgt cgatgacgcc gggcgcccgg tcgatcagct
     1801 cccggataag ctccgcagtc gcgccttccc tttccttcgg acctcccaca atgaagttcg
     1861 tggcgatctg cgcgaggccg cagtcggcgg cgtacgagac gaattccaga atgtcgtcga
     1921 tgcgtacgtg tttattgtac tggtcgatga cgttctgatc accggattct attccgatct
     1981 gcagacggat cagcccggcg tcgatcatga cgggcaggac atccataaag cttttgatgc
     2041 gccccacatg gcattcgcag taccagacca gatccagttc tttccgcagc tcccgcatcc
     2101 cttcacagaa ggcttctatg cgctccctgc cgagggtaaa ggtgtcgtcc atcacaatga
     2161 ggcatcgcag gccgggattg ttgcggaggt ttgttttgat ttcctcgaac accaggggga
     2221 tgttgcgcag cctgaccttg cgtttgccgg ggctgggggc gcagtaggcg cactggaatg
     2281 ggcaccctct gccggtgaag atcaagttcc cgtaggcgcg gtcgctgtgc agcgaggtgt
     2341 ggtaggccgg caggggcagg gcgtccggat cgtcgatgat ctcgggccgt gtctcgcgaa
     2401 atacgccgtc cttgatcatc gcgagcccct tcacctcgtc gagttcgccc tcgcccttca
     2461 agaccgcgtt gaggagcagc ggcagcgaaa tttcgccttc accccgcagg atatagtccg
     2521 cgccgctgct ttcaagaaac gtccttccca aggcgatgct ctgggggccg cccacaacga
     2581 cgggaatccc ccagcgctcc tttatctttt gggagacgtc ttcgatgatc cggacgttgt
     2641 cgaaatcgca ggagaatccc accgcttcgg ggcggtgtct cgcgatctca tccaggaggc
     2701 cttccgcctt tccgtgaaag accagaggct cgaatcctgc cgcctccaag gcgcccgcca
     2761 ggtaaaagag ccctacataa tcatgggcgg ttgtttcgta ttttttcgac acgttgcata
     2821 gcagtatctt catcacggcg ccttcttagt acggaaccac aagcagccag tattttttat
     2881 cgaacgcctt caaggtctcg acgatgcgcc ccgtcagatc ctccgtcgtt tcttcgaagg
     2941 gcttgccgaa tcgctcgtag aggatgccgg ctatctcggc gatggtgctc ttacctgtgg
     3001 agtatttcaa cacctcgtac tcgaaaggcg acagagcctc cccccgcgca agggggtagc
     3061 cccgggagaa gtccacgtcg cgccacattt cgagcaggcg ttgtggacgc cacttgggca
     3121 gttccccagg ctggacgtcg ccgatgcggg aggccgcttc ccgtgccagc atggtgtagt
     3181 agggggccag catgggtttg tgatgagcta tgatgttgct ccagtatttg ggaccttttg
     3241 aattcatgga agtgcggaaa gctctcagaa tatcctcgtg accgagttcg ccggtatcga
     3301 gcatcgcctt cacctcgttc acaaaactct gaatgtactc ctgataggcc gccatgattt
     3361 cccgccggtc catcgacgcg ctctccataa ggggataatc ggagatggat gccacccctt
     3421 gcctgtcata gatcttcagt ccgtattttt cggggtggcg cccgatatcg gtcatcggat
     3481 agggcatgag gatactgggg gcaatctcca tcatccccgg tcccaggcgc atcagcctgg
     3541 cggcaaaggc cttgttcttt tcaatatgct cacggttctc aaaaggaccg cctaccagaa
     3601 gggccccgaa aacctgctgc actccggcat cgtaggcggc tttcaccacc gtttccacca
     3661 tttccggagc gaggttcttc cggtatatcc tgagcatctc gcggtctgcg gattcgatgc
     3721 cgatttggag ccgattgagt ccggccgcca ccatgtcctt tatgacctcg ggtcttttgc
     3781 tcagggacgc cgcatccgcc tcgcaataga actggaaggg cctgacggcc tgaagcgcct
     3841 ttacctcccg gcagaattca tccagccatt cgggattggc gaccagcgta tcgtcgcaga
     3901 ataaaaagta cttgagttca gggtacttga ggaggagatg acgcatttcc gcaacgacgt
     3961 tcgcgacgct ccgccggcgc acggggcgtt tcaaggcgcc ttcatgacaa aaaacgcagc
     4021 tgtaggggca cccccggctg gtcatgatgt acaggttgga gtaatcgacg ggcgtcatgc
     4081 ttttctcgta ggctgggaag ggcagctcgt ccagattttc caccaccggc ccttcgcccc
     4141 tgtcgacgca ctcgccgttt tcgtcgataa agaagacccc ggggatgtct tccaggctct
     4201 tcttggtt
//
LOCUS       DAOTIL010000132         4203 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminobacteriaceae bacterium isolate AMR_MDS_3026
            NODE_25029_length_4203_cov_3.598843, whole genome shotgun sequence.
ACCESSION   DAOTIL010000132 DAOTIL010000000
VERSION     DAOTIL010000132.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868050
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminobacteriaceae bacterium (wastewater metagenome)
  ORGANISM  Aminobacteriaceae bacterium
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae.
REFERENCE   1  (bases 1 to 4203)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4203)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:19:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,224
            CDSs (total)                      :: 3,198
            Genes (coding)                    :: 3,184
            CDSs (with protein)               :: 3,184
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4203
                     /organism="Aminobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_25029_length_4203_cov_3.598843"
                     /isolate="AMR_MDS_3026"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:3034609"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1887
                     /locus_tag="P5201_03895"
     CDS             <1..1887
                     /locus_tag="P5201_03895"
                     /EC_number="6.1.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008520955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0004832 - valine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006438 - valyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="valine--tRNA ligase"
                     /protein_id="HRV97705.1"
                     /translation="DPEFGTGCVKITPAHDPNDFLVGQRHNLEQVQVIDENGIMNSLA
                     PGYEGLNIEEAREKAVEDLQASGHLGTIEEIVHSVGHCYRCHTTVEPYLSEQWFVKAK
                     PLAEAGVKVVQDGKIRWVPDQWEKVYYQWMENIRDWCISRQLWWGHRIPAWTCGACGH
                     ITVSESDPTVCERCGSAAIVQDEDVLDTWFSSALWPFSTLGWPEKTEELAYFYPTSLM
                     VTGFDIIFFWVARMIMMGLEFMDEIPFHDVYIHALIRDEKGQKMSKSSGNVIDPLDMI
                     EKYGADALRMTLAALTVQGRDILLSTSKIETYRLFMNKIWNASRFALMNLEGLPHEAV
                     LSDDSELALHDRWILTRTSQVAGETTRLIEAFGIGEAARMLYDFVWGDLCDWYLELSK
                     PALKGAEGPGRKLATQLVIASVFKDVLLLMHPFIPFVTEELWQVFGFGKDAGGSSPFM
                     ECHQWPTGDDAFSFPESLEKMAAFQEVVRVLRNLRAEARVTPQTFVNRAVLQCGEESS
                     LSLIIHETAPLMQDLTKIREISLLPLSADKPAGALSSQITGGEVSLIVGDILDIASEI
                     ARLSEEIASVTKNMQACSRKLENEDFVRRAPADIVEKERLRLEENEARLRRIHENIES
                     LKRS"
     gene            1890..3272
                     /locus_tag="P5201_03900"
     CDS             1890..3272
                     /locus_tag="P5201_03900"
                     /EC_number="6.3.2.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR01499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004326 - tetrahydrofolylpolyglutamate
                     synthase activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008841 - dihydrofolate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006730 - one-carbon metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="folylpolyglutamate synthase/dihydrofolate
                     synthase family protein"
                     /protein_id="HRV97706.1"
                     /translation="MMKRPFSTFEDLEDFFSRLASPGIRPGLERIARLLKQLGNPEKR
                     FSAIHIVGTNGKGSTAAFTERILRESGYTTALYTSPHLESPAERLLFSGVPLSLEEWS
                     ACAFEVEDALNDDPTLREDPPTFFEIVTATAFLLCEHRGVDIAVVEAGLGGRLDATNL
                     LGNICLTLITSISMDHMDFLGDTLESIAGEKFAVMRTRTPAVFSGTPAQLVPLFRSTA
                     NELGALPNTTAELCSCEEIRNESGRLSYVYRLLHPALLSSIGGEAKSFDQNAVIPVST
                     SLRGMYQIENSVLAISGILLLVSCFPKITSNTIRRGIAAARWPGRFETIDSTPPLVLD
                     GGHNPGGVEKLTESLGAVYGGKKIGVVYGCMKDKAYPECLELLRKVCSSLYCVSVPEN
                     PRAAQPELLAETARSHGWKPEDISVLHDPLEAIRVASLSNEVVVCCGSLYLIGYVKSR
                     LSFLKKERNE"
     gene            3383..4027
                     /locus_tag="P5201_03905"
     CDS             3383..4027
                     /locus_tag="P5201_03905"
                     /inference="COORDINATES: protein motif:HMM:NF014619.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyphenol oxidase family protein"
                     /protein_id="HRV97707.1"
                     /translation="MDRALGNPATARELTELLIPRQGALIAPHQVHGTFMVPGEVSYA
                     LPERPEGDGVFLDTPGVEGSLRFADCFPVVLASLAPSPWIAIVHSGFKGAVSNISGTV
                     CENIFSANGRRPAATYAWVGPGIGRRSYNRKQNDPWTKKGLSVFSPENRDVRSADVSF
                     DIGGQIFRQLTDAGLLPENICSLPLCTFERSDIFYSYRNGDMENRLFLLAFLTE"
BASE COUNT          933 a         1081 c         1186 g         1003 t
ORIGIN      
        1 gacccggagt tcggcacggg gtgcgtcaag ataactcctg cgcacgatcc caacgacttc
       61 ctggtgggac agcggcataa ccttgaacag gtgcaggtta tcgatgaaaa cggaatcatg
      121 aactctcttg ctccgggata cgaggggctg aacattgaag aggcgcggga aaaagcagtc
      181 gaagatcttc aggcttcggg acaccttggt acgatagagg agatcgtgca ctcggtgggg
      241 cattgctatc gctgccacac cacggtcgag ccctatcttt ccgagcagtg gttcgtcaaa
      301 gcgaagccgc ttgccgaggc tggagtcaaa gtcgtacagg acggaaagat acgatgggtg
      361 ccggaccagt gggagaaggt ctactaccag tggatggaga atattagaga ctggtgcatc
      421 tcacgccagc tctggtgggg acacagaatc ccggcgtgga cctgtggagc gtgcggtcac
      481 atcacggttt ccgagtcgga tccgacggtg tgcgagcgct gcggaagcgc cgctatagtc
      541 caggatgaag atgtcctcga cacgtggttc agcagcgcgc tgtggccctt ttcaacgctc
      601 ggatggcccg aaaagaccga ggagcttgcg tacttctatc cgacctctct tatggtgacc
      661 gggttcgata ttatcttctt ctgggtggcg cgcatgatca tgatggggct ggagttcatg
      721 gacgagatac cgttccatga cgtctatatc cacgcgctta tccgcgatga aaaagggcag
      781 aagatgagca agtcgagcgg gaacgtcata gatccgctgg acatgattga aaaatacggt
      841 gcggatgctc tccggatgac gcttgccgcg ctcaccgttc agggaaggga tattctgctg
      901 agcaccagca agatagagac atacaggctc ttcatgaata aaatttggaa tgcgtcgcgt
      961 ttcgccctta tgaatctgga gggccttccg cacgaagcag tcctttcaga tgattcggaa
     1021 ctcgcgctcc acgacagatg gattcttacc cggaccagcc aggtcgccgg agagacgacg
     1081 cggcttattg aagccttcgg tatcggcgag gcggcaagga tgctgtacga cttcgtctgg
     1141 ggagaccttt gcgactggta tctggagctt tcaaaaccgg cgctcaaagg cgcggaaggt
     1201 cctggccgga aacttgcgac gcagcttgtc atcgcatccg tctttaaaga tgtactcctt
     1261 ctcatgcacc ccttcattcc gttcgttacg gaggagttgt ggcaggtttt cgggtttgga
     1321 aaagacgccg gcggaagctc tccgtttatg gaatgtcatc agtggccgac aggcgacgat
     1381 gcattctcct tccccgagtc gctggagaag atggcggcgt tccaggaagt cgtcagggtg
     1441 ctccgcaacc ttcgcgcaga ggcgagggtg acgccgcaga cctttgtgaa ccgcgctgtt
     1501 cttcagtgcg gcgaggagag ttcgctgtcc ctcattatcc atgaaacagc gccgcttatg
     1561 caggatctga caaaaatccg tgagatatcc ctcctgccgc tctccgcgga caaacccgca
     1621 ggagcgctct cttcccagat aaccggagga gaggtcagtc ttattgtagg ggatatactc
     1681 gacatcgctt cagaaatagc ccgtctttcg gaagagatag cgtctgtgac aaagaacatg
     1741 caggcttgca gccgaaagct cgagaacgaa gatttcgtcc gtcgcgctcc ggcggatatc
     1801 gtcgagaagg aacgtcttcg tctcgaagag aacgaagctc ggcttcgtcg tattcatgag
     1861 aatatcgaaa gcctcaagcg ctcctaaaga tgatgaaacg cccgttctcg acctttgagg
     1921 atttggagga tttcttttcc cgcctggcga gccccggaat acggccgggg ctcgaacgga
     1981 ttgcccgtct gcttaagcag ctgggcaatc cggaaaaaag gttttccgcc atccatatcg
     2041 taggaacgaa cggcaaagga tcaactgctg ctttcacaga gcgcatcctc cgggaatcag
     2101 gatatactac tgctttgtat acgagtccgc atcttgagtc tccggcggaa cgtcttctct
     2161 tcagcggggt tcctctctcg cttgaagagt ggtctgcctg cgcttttgaa gtcgaagatg
     2221 ccttaaatga tgatccaacg ctccgcgaag acccgccgac gttctttgaa atcgtaacgg
     2281 cgactgcgtt tctgctctgt gaacacaggg gcgtcgacat agccgtggtc gaagccggtc
     2341 tggggggaag actggatgcg acgaatctcc tcggcaatat ttgcctgacg ttgatcacgt
     2401 ctatttcaat ggatcatatg gactttctgg gggatactct cgaaagtatt gccggggaaa
     2461 agtttgcggt aatgcgaacg agaacgcccg ctgtcttttc gggaactcct gcgcagcttg
     2521 ttcctctctt tcgatcgacc gcgaatgaac ttggcgcgct cccgaacacc actgccgaac
     2581 tctgctcgtg cgaagaaatt cgcaatgaaa gtggaagatt gtcgtatgtc taccgtctgc
     2641 ttcatcctgc gttgctgtct tcaataggtg gggaagctaa atccttcgat cagaatgccg
     2701 tcatcccggt ttcaacgtcc ttgcgcggta tgtatcagat cgaaaacagc gtgcttgcca
     2761 tttcagggat cctcctcctc gtatcgtgtt ttccgaaaat tacatccaat actatccgcc
     2821 ggggaatcgc cgctgcgcgt tggccgggca gattcgaaac gatagactca acccctccgc
     2881 tggttcttga cggcgggcac aatcccggcg gcgtagagaa gctgacggag agtctcggtg
     2941 cggtctacgg cggaaagaag atcggcgtgg tgtacggttg tatgaaggat aaagcgtatc
     3001 cggagtgtct ggaactgcta cgaaaagtct gctccagcct ctattgcgtt tcagttccgg
     3061 agaatcctcg cgctgctcag cctgagcttc tcgccgagac tgcgcgttca cacgggtgga
     3121 agcctgaaga tatttcagtt ttgcacgatc ccctggaagc cattcgtgtc gcctcccttt
     3181 cgaatgaagt tgttgtctgt tgcggcagtc tgtatcttat tggatacgta aagagcagat
     3241 tgtcttttct caaaaaggag agaaacgaat gatcgcgcct tctttcagaa tcgagcccaa
     3301 tggagacttt ccgtttctcg aatatctctt tccggagccg catacgcctt tttttatccg
     3361 cctttttcta cggggggcgg ccatggatcg tgctctcggg aatcccgcga cggccaggga
     3421 gcttaccgaa ctgttaatac cgcgccaagg ggcattgatt gctccccatc aggtgcacgg
     3481 cactttcatg gttcccgggg aggtttcgta tgctttacct gaacgaccgg aaggggatgg
     3541 cgtttttctt gacactcccg gtgtcgaggg atctcttcgt tttgccgact gtttccccgt
     3601 tgttcttgct tccttggcgc catcgccctg gatcgccata gtgcactccg gattcaaagg
     3661 ggcggtttcc aacatcagcg gtactgtctg tgaaaacatc ttctctgcga acggacgtcg
     3721 gcccgccgca acgtatgcct gggtggggcc aggaatcgga cggaggagct ataatagaaa
     3781 gcagaacgac ccctggacga aaaaagggct ttctgttttc tctcctgaaa acagagatgt
     3841 acgttctgct gatgtgtctt ttgatatcgg agggcaaatt ttccgccaac tgaccgacgc
     3901 ggggctctta cctgagaata tctgttccct tccgctgtgt acctttgaga ggagcgatat
     3961 attctactcc tacaggaacg gggatatgga aaacagactt tttctcctcg cttttttgac
     4021 ggagtaaaaa ataccatttt tactccgttt accgggagaa ttatgtaaga gcacgaaaag
     4081 gccgtacgta atcaaaggag ttgaagagtc catggacttg aaacgagtag gcgtgatcgg
     4141 agtaggccat cttggccagc atcacgcaag agtctacacg gaactgctcg gcgcccatct
     4201 tgt
//
LOCUS       DAOTIL010000133         4180 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminobacteriaceae bacterium isolate AMR_MDS_3026
            NODE_25213_length_4180_cov_4.128242, whole genome shotgun sequence.
ACCESSION   DAOTIL010000133 DAOTIL010000000
VERSION     DAOTIL010000133.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868050
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminobacteriaceae bacterium (wastewater metagenome)
  ORGANISM  Aminobacteriaceae bacterium
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae.
REFERENCE   1  (bases 1 to 4180)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4180)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:19:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,224
            CDSs (total)                      :: 3,198
            Genes (coding)                    :: 3,184
            CDSs (with protein)               :: 3,184
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4180
                     /organism="Aminobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_25213_length_4180_cov_4.128242"
                     /isolate="AMR_MDS_3026"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:3034609"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..536
                     /locus_tag="P5201_03910"
     CDS             <1..536
                     /locus_tag="P5201_03910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013047791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HRV97708.1"
                     /translation="GVSRTPVREALRRLASEGLVLIIPNSGARLAAPTIREIEDTFLV
                     REQLECLAIALAAERIGERHLRRIEEALVEEARAIEDKDLETYLEVNEAFHKTVADAS
                     GNRVLAEYVENILARTNAYVVFYDPFYHVETSPSPDDHRDILASLRAHDKEKSVALMK
                     AHLEEAVAGLGKIVVEE"
     gene            702..1835
                     /locus_tag="P5201_03915"
     CDS             702..1835
                     /locus_tag="P5201_03915"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006300190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DegT/DnrJ/EryC1/StrS family aminotransferase"
                     /protein_id="HRV97709.1"
                     /translation="MATKIPTLDLVRNYSRIKDEILEAVHGVLETQHFILGPDVSLFE
                     KECEQYLGGVSAVACASGTDALILALMSLDIKEGDEVITTPYSFFATASCITRLGAVP
                     VFVDVDPATFNVDMNKVLEATTPRTKVFLPVHIFGQMAPLEDGMKELTSRGITVVEDA
                     AQAFGAWRSVEGDILRAGAVGRIGCFSFFPTKNLGGYGDGGMVVSTDTQLADRLRKLR
                     VHGAGTTYFHDEVGLNSRLDSLQAAILRVKLRHLEEWNEERRIVAGRYYALFAEKGLL
                     ETVQPPVELEGNHHIYHQYVVRVPQRDDLQRFLEEEGVVSRVYYPLSLHLQRCFSFLG
                     YKQGDFPVSERLSKETLALPVFPELSLEEQRRIVAAIGKFYGK"
     gene            1949..2644
                     /locus_tag="P5201_03920"
     CDS             1949..2644
                     /locus_tag="P5201_03920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005659415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc metallopeptidase"
                     /protein_id="HRV97710.1"
                     /translation="MFPFFFDPTMILLIPALLLAMWAQMRVKSVFANYSRVGSRRGVT
                     ASAVARALLDRFGLRDVPVQRVGGNLTDHYDPRDKSLSLSDSVYESSSIAAIGVAAHE
                     VGHAIQDSVNYSPLKIRNAMVPVVGIGSTLAFPLFFIGILLRGQMMMDLGIVLFLGVI
                     LFHLVTLPVEFDASNRALRVLADTGMLSTDEIGGAGAVLRAASWTYVAATVMAFAQLI
                     RLLFLRGMYGSRD"
     gene            2744..3427
                     /locus_tag="P5201_03925"
     CDS             2744..3427
                     /locus_tag="P5201_03925"
                     /inference="COORDINATES: protein motif:HMM:NF039426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6391 domain-containing protein"
                     /protein_id="HRV97711.1"
                     /translation="MPLLFFLIFIFIFPWFFLLLVLIFFVLIPLGFSIQSFLWILTRP
                     AQLFRIVTSRQVRKNHALAHGTLRILEEEYGRLNAEGMPVEDGFSLRGGVDPETALAA
                     AKTALDRIRRGDRSLAFFHRCGITVVMANTLFAFLLFFVLRMLGLFSFLSAILCLVAV
                     YFAGSRLSYLAQVYGTISPDLDDMEITGVDVRNEQFALGGLSFLTPSSIFIRTRRAGE
                     PVIAEVVSP"
     gene            3763..>4180
                     /locus_tag="P5201_03930"
     CDS             3763..>4180
                     /locus_tag="P5201_03930"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="Sun protein"
                     /protein_id="HRV97712.1"
                     /translation="MNAVLRRVSEEGRNELARLESSGNSKEQALVAGIPPWVASYWRN
                     TIGDEAKKLLKMARIRPYSSFRILPEDKMDAILEQAKNSGICCWPSPLVPYSIRLSST
                     IYPGNFPGYSEGWSTPQSESSMLVGEVMKKVYKGGAI"
BASE COUNT          906 a          983 c         1153 g         1138 t
ORIGIN      
        1 tgggggttag cagaaccccg gtaagagaag ctcttcgccg tcttgcaagc gaagggcttg
       61 tccttatcat accgaacagc ggcgcccggc ttgcggctcc tactatccgg gagatagaag
      121 atacatttct tgtgcgcgag cagttggaat gtcttgccat tgcgcttgcc gccgaacgta
      181 ttggagaacg tcatcttcgc cgcatagagg aggccctggt cgaagaggcc agagcgatcg
      241 aagataaaga tcttgaaact taccttgagg tcaacgaagc gttccataag acagtggcgg
      301 atgcaagcgg gaacagggtc ctcgcggagt acgttgagaa tatcctcgca cggacgaatg
      361 cctacgtcgt gttttacgac cccttttatc atgtggagac ttcgccgagc cctgacgacc
      421 acagagacat cctggcaagt ctcagagctc acgacaaaga gaagagcgtc gctttgatga
      481 aagcccactt ggaagaggct gttgcgggcc ttgggaagat tgtcgtcgaa gagtagacta
      541 ctttcttcat tctcaagttg tttaggcgat cgccccggac ggagcgttcg cttgtttttt
      601 tgctcaatgc caaaattcgc cgaatgaagt gaagatattc aatggagttc ttacgagagc
      661 gagaccccgt ttcgctcact tttacgggag gtactttttt catggcgacg aaaataccca
      721 cgctagatct tgttcgtaat tattcaagaa taaaagacga gattcttgaa gcggtgcacg
      781 gagtgcttga gacacagcat ttcattctcg gaccggatgt ctctctcttt gaaaaagagt
      841 gcgagcagta ccttggaggc gtgtcagcag ttgcgtgcgc gtctggaaca gatgcgctga
      901 ttcttgcgct catgtcgctc gatataaaag agggggatga ggtaatcaca acgccgtaca
      961 gcttctttgc aacggcaagc tgtattacac gtcttggggc tgtccccgtt tttgtcgacg
     1021 tcgatcctgc gacattcaat gtcgatatga acaaagttct tgaggcgacg acgccgcgaa
     1081 caaaagtttt tcttcccgtg catattttcg gccagatggc gcccctcgaa gatggcatga
     1141 aagagcttac ttcccgcggt attacagtgg tcgaagacgc agcgcaagct tttggggcct
     1201 ggcgttctgt tgaaggggac attcttcgag ccggagcagt cgggcgcatc ggttgttttt
     1261 cattcttccc taccaaaaac ctgggaggat acggcgacgg cgggatggtt gtctcgacgg
     1321 atacgcaact ggcagatcgt cttcgcaagc tgagggttca cggcgccgga actacctatt
     1381 tccatgatga ggttggcttg aacagccgtc tcgactcgct tcaagcggcc attttgcgtg
     1441 taaaactccg ccaccttgag gagtggaacg aagagcggcg tatcgtcgcc ggaagatatt
     1501 atgctctttt tgcggaaaaa ggtcttctgg aaacggtgca accgccagtg gaactcgaag
     1561 gcaatcatca tatttatcat cagtatgtcg tacgggttcc gcaaagagac gaccttcagc
     1621 gtttccttga ggaagaagga gtagtaagca gggtctatta tccgctgagc cttcatctgc
     1681 aaaggtgttt ctctttcctt ggttataaac aaggcgattt cccggtttca gaacggctta
     1741 gcaaggaaac tcttgctctc ccggtcttcc ctgaactctc tctagaggag cagcggcgta
     1801 ttgttgcagc aatcgggaaa ttttacggaa aatagtctga ccaatattgc tctttctcat
     1861 acaaccgtgt aaaataccga tggaggaaag gggaacgcag tgtacccttt cctcgtagaa
     1921 tgagagcata tagcggaggt gagaaacagt gtttcctttt ttctttgatc cgacgatgat
     1981 attgctgatt ccagcgcttt tactggctat gtgggcgcag atgagagtga agagcgtctt
     2041 tgcaaactac agccgcgtcg gctcccgtcg gggagttact gcatcggctg tcgcgagggc
     2101 cttgcttgat cgatttggtt tgagagatgt ccctgttcaa agagtaggcg gcaacttgac
     2161 ggatcactac gatccccggg ataagagcct gagtctttcg gattcggtat atgaaagttc
     2221 aagcatcgct gctattgggg tagctgcgca cgaagtcgga catgcgatcc aggacagcgt
     2281 gaattactcc cctttgaaga tccggaatgc tatggtaccg gttgtcggga ttggctcgac
     2341 gcttgcgttt ccccttttct tcataggaat tcttcttcgg gggcagatga tgatggatct
     2401 tgggattgtt ctcttccttg gggtgatact cttccatctg gttaccttgc cggttgagtt
     2461 cgacgcgagt aatcgcgcgc tccgtgttct tgccgatacg ggaatgctgt caaccgatga
     2521 aataggagga gcaggagcgg ttttgcgcgc agcatcgtgg acctatgttg cggcgaccgt
     2581 aatggcattc gcccagctta ttcggcttct ctttctccgt ggtatgtatg gaagcagaga
     2641 ctaaaaacgc gttcagcttc gccgagaggc gatggtctgg gaaatggagc ggctttcccg
     2701 gtcgctccat tttatttcct cggggagaaa ggaccagaat cccatgcctt tgcttttttt
     2761 cttgattttt atttttattt tcccttggtt ttttctgctg cttgtcctca tcttttttgt
     2821 gcttattcct cttggttttt cgatccagtc gtttctctgg attcttacac ggcctgcaca
     2881 gctttttcgt attgtgacaa gccgtcaggt tcgtaaaaac cacgcccttg ctcatggcac
     2941 gctccgtatt cttgaagagg agtacggacg attgaacgct gaaggtatgc ccgtcgaaga
     3001 cggattttct ctcagaggag gagtcgatcc ggaaacagca ctcgcggctg cgaagactgc
     3061 tttggatcgt atccgaaggg gtgatcggtc gctggcgttt tttcatcgtt gcgggataac
     3121 tgtcgtgatg gcaaatacgc tttttgcttt cctccttttt tttgtgttgc gcatgcttgg
     3181 tctgttcagt ttcctttcag ctattctctg ccttgtagcg gtgtactttg ccggatctcg
     3241 cctcagttat ctcgctcaag tgtacggcac gatttcgcct gatctggatg atatggagat
     3301 tacgggagtg gatgtacgaa acgagcaatt tgcgctgggg gggctctcct ttcttactcc
     3361 atcaagtatt tttattcgga caagacgagc cggcgagcct gtgattgccg aggtggtctc
     3421 gccttgagag ggattgaggc tgctcttcat gtctggagtg aagtgcggga aggcaaattc
     3481 gcttccgagg ttatcagacg cctggcagat tcaatgacgc ctggagatct tatgcttgcg
     3541 tcgacccttg tgtatacagc cctcaggaga cagtttctct ggaaagagat ctacgcgcat
     3601 tttttgaagg cgtctcccgc cggcgtttct cgtcttacag acgatgctct ttgcattggg
     3661 accgcaggtt tgcttgagct gaggaagttt gctccgcgtg ttttggtaaa tgctcttgtg
     3721 cagtatatga aagataaagg ggacgaaaga ggcgctcggc tggtgaacgc cgttcttcga
     3781 agggtgtctg aagaagggag aaatgaactt gcgcggctcg agtcttccgg caacagcaag
     3841 gaacaggcgc ttgtggcagg tatccccccg tgggttgctt cgtattggag gaacactatc
     3901 ggcgatgagg caaaaaaact cctgaaaatg gcgcgtattc gtccatattc ttcgtttcgc
     3961 attcttccgg aggacaaaat ggacgctatc ctggaacaag ctaaaaacag cggcatttgc
     4021 tgttggcctt cccctcttgt cccgtattca attcgcctca gctccaccat atacccgggc
     4081 aacttccccg ggtattccga gggatggtct acgcctcagt cggagtcctc gatgctcgtc
     4141 ggcgaggtga tgaaaaaagt atacaagggc ggagctattt
//
LOCUS       DAOTIL010000134         4172 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminobacteriaceae bacterium isolate AMR_MDS_3026
            NODE_25277_length_4172_cov_3.137965, whole genome shotgun sequence.
ACCESSION   DAOTIL010000134 DAOTIL010000000
VERSION     DAOTIL010000134.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868050
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminobacteriaceae bacterium (wastewater metagenome)
  ORGANISM  Aminobacteriaceae bacterium
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae.
REFERENCE   1  (bases 1 to 4172)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4172)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:19:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,224
            CDSs (total)                      :: 3,198
            Genes (coding)                    :: 3,184
            CDSs (with protein)               :: 3,184
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4172
                     /organism="Aminobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_25277_length_4172_cov_3.137965"
                     /isolate="AMR_MDS_3026"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:3034609"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1026)
                     /locus_tag="P5201_03935"
     CDS             complement(<1..1026)
                     /locus_tag="P5201_03935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015556624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0000902 - cell morphogenesis [Evidence
                     IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein"
                     /protein_id="HRV97713.1"
                     /translation="MFKFLTGVFGGDVGIDLGTANIVVYVKGRGIVISEPSAVAIRKR
                     PRGGATEVIAVGREAKAMAGKTPGGVFTIWPLQDGVIANFEMTEELIRHCLRRANGGK
                     PFVSHPRVVISVPAEVTEVERKAVIDATLGAGAKEAYVIEEPVAAALGVGLPIQEPRG
                     SMILDIGGGTSEVGVLSLGGIVVTNSLRAAGKEMDNAIVAMLRQRYALLIGDTTAEEV
                     KIAIGSALPLDKELEMAVKGRDLADGLPKADVVTSVEVREALEPILLRIEDMVKVALE
                     QTPPELAKDIVDQGIFLSGGVSLLRGLAERLSKAMNTPVVVAEDPLFAVARGVGKILE
                     NLGDMKKV"
     gene            complement(1046..1717)
                     /gene="radC"
                     /locus_tag="P5201_03940"
     CDS             complement(1046..1717)
                     /gene="radC"
                     /locus_tag="P5201_03940"
                     /inference="COORDINATES: protein motif:HMM:TIGR00608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadC"
                     /protein_id="HRV97714.1"
                     /translation="MKLASLPREEMPRERLRLRGASSLSLPELLAILLRTGSRGKDVL
                     ELAADVLNEFGGAKGMARATEEEMLGFPGLGDAKACVLLAALELGKRLEAARRELQEG
                     KDWRRSVDEIAGELAFEEREYIFAIHVRSNGSVIHVDRISFGGAEGAYLDVKYLLRRA
                     VRMDSYGLVLVHNHPDGSLQPSREDRLLTDFVSRRTKILDIHLLGHFVVANGESHGIA
                     LPGEP"
     gene            complement(1714..2364)
                     /locus_tag="P5201_03945"
     CDS             complement(1714..2364)
                     /locus_tag="P5201_03945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013048000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HRV97715.1"
                     /translation="MKYTRFPLGPLWTNSYLVWDASGDGFLVDPAGDAAEILSFAREN
                     NIVVRAVLLTHGHLDHVGGLPELLEKIASPVYIGEGDAEMLRSPDQNAAEWMGYDFKG
                     IRNFFTLRDGEQLPVGAMSVSVLETPGHTPGCVCYHVSESSDTGEETLLLSGDTLFAR
                     SVGRTDLPGGNGERLHESLIRLAALPDTLRVLPGHGPETTIGAERIHNPFWPEDKA"
     gene            complement(2473..2910)
                     /gene="dtd"
                     /locus_tag="P5201_03950"
     CDS             complement(2473..2910)
                     /gene="dtd"
                     /locus_tag="P5201_03950"
                     /EC_number="3.1.1.96"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008521901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004045 - aminoacyl-tRNA hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-aminoacyl-tRNA deacylase"
                     /protein_id="HRV97716.1"
                     /translation="MRAVVQRVSSARVSVDGETKGAIGKGVCVLLGVSDADGEQEADW
                     MAEKIVNLRIFEDDEGKMNRSLLEVGGSMLAVSQFTLYGDCRKGRRPSFVGAGEPGHA
                     NRMYESFKSAAAGRGVEVESGVFQAHMLVEIANDGPVTLIIDR"
     gene            complement(2918..>4172)
                     /locus_tag="P5201_03955"
     CDS             complement(2918..>4172)
                     /locus_tag="P5201_03955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013047998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="TGS domain-containing protein"
                     /protein_id="HRV97717.1"
                     /translation="QSLHTTVVGPTGEPLEVQIRTWDMNSLAEYGIAAHWRYKEGGAS
                     LDDLDAKLTWIRQAIEADHDGSEPSEFLERLKEDVLSSDVFVFTPQGEVVSLPKGSTP
                     LDFAFAIHTQVGTRCVGAMVNNRIVAMGYELQNGDIVKIITSPQGAPSRDWLKIARSS
                     KARSKIRAYFRALEKTERLEKMQRGRDLLEREVKRRGTPEFEEFDELLPLLNKIARDT
                     AYSNGEELLSAIGGGHQSVSLVAQRLAGKPQPQPAPVQSLPPEPEKHTSFSGKKGDSD
                     IVVEGADGVQVVLSNCCEPVPGDPIIGFSTRTRGITVHRADCENVRNSREERRVSVSW
                     GMTGTRRFSARLKIEAVDRAGLFGDVAQAITAGDGSMTGIKAGVVGGNQARMKLEVRV
                     RDLEHLYLIVARLNAVKGMIQVSRG"
BASE COUNT          757 a         1411 c         1168 g          836 t
ORIGIN      
        1 caccttcttc atgtcgccga gattctccag aatcttcccc acgccgcggg ctaccgcgaa
       61 gagcggatcc tccgccacca ccacaggggt attcatcgcc ttggacaggc gttcggccag
      121 acctcgcagc agcgagacgc cgccggacag aaagatcccc tggtcgacga tgtccttggc
      181 cagttcggga ggcgtctgtt cgagcgccac cttcaccatg tcctctatgc gcagcagaat
      241 cggctccagc gcctcgcgca cctccaccga ggtcacgacg tcggccttcg gaagcccatc
      301 tgcgaggtcg cgccccttga cggccatctc aagctccttg tcgaggggaa gcgcggatcc
      361 gatggcaatc ttgacctcct ccgccgtcgt atcccctatg agcagcgcat atcgctggcg
      421 gagcatggcg acaatggcgt tgtccatctc cttcccggcg gcgcggagcg agttggtcac
      481 gacgatgccg ccgagagaga gaacgccgac ttcgctcgtc cccccgccga tgtcgaggat
      541 catgctccct cgcggttcct ggatcggcag tcccacgccg agcgccgccg caacaggctc
      601 ctcgatgacg tacgcctcct tggcgccggc cccgagcgtt gcgtcgataa cggccttccg
      661 ctcgacttcg gtaacctccg cgggcactga gatcacgacc cgcggatgag agacgaaggg
      721 tttccccccg ttggccctcc tgagacagtg acggatcagt tcttcagtca tttcgaagtt
      781 cgcgatgacg ccgtcctgca gcggccagat agtgaagacg cctccgggcg tttttccagc
      841 catcgccttg gcctcgcgtc cgacggcgat cacttccgtc gcccctcctc tggggcgctt
      901 ccgaatagca acggcggacg gctcgctgat gacgattccc cgccccttga cgtacacaac
      961 tatgttggca gtcccgaggt cgataccgac gtcgccgccg aacactcccg taagaaattt
     1021 gaacacctgc ttcagtcccc gctttctagg gctctccggg gagagcgata ccatggctct
     1081 caccgttggc cacgacaaaa tgtcctaaaa ggtgtatatc caggatcttt gtcctcctcg
     1141 atacaaaatc agtcagcaga cgatcctcgc ggctcggctg cagcgacccg tcgggatggt
     1201 tgtgcaccag caccaggccg tacgaatcca tccggacagc tcttcgtaac agatatttta
     1261 catcaagata ggcgccctcg gcgcctccga aggaaattct gtccacatga atcacggatc
     1321 cgttgctccg gacgtgaatg gcaaagatat attcccgctc ctcgaaagcc agctctccgg
     1381 ctatctcgtc gacgctccga cgccagtcct ttccctcctg aagctctctc ctcgccgctt
     1441 cgaggcgctt tccaagctcc agggcggcaa ggagaacgca ggccttcgcg tcgccgagtc
     1501 cgggaaaacc gagcatctct tcctccgtag cccgcgccat tccttttgcg cctccaaact
     1561 cgttgagcac atccgcagcg agttcgagga catccttccc cctgctcccg gttctgagca
     1621 gaatcgcaag cagctccgga agcgagagcg acgaggcgcc gcgaagtcga agccgctccc
     1681 gcggcatctc ctcgcggggc agcgaggcca gtttcacgcc ttgtcctccg gccagaacgg
     1741 gttgtggatg cgttccgctc cgatagtggt ttcgggaccg tggccgggca gcacccggag
     1801 tgtatccgga agcgcggcca ggcgaatcag cgattcatgc agtctttccc cgtttcctcc
     1861 gggaaggtcc gtacgcccga cgctgcgggc gaagagggtg tctccggaga gaagaagggt
     1921 ctcctctccc gtatccgagg actccgatac atgatagcag acgcatccgg gcgtatgccc
     1981 cggggtctcc agcacggaga ccgacatggc gccgacggga agctgttcac cgtccctcag
     2041 cgtaaaaaag ttcctgatcc ccttgaaatc ataccccatc cattccgccg cgttctgatc
     2101 gggtgagcga agcatctccg cgtccccctc tccgatgtac accggggaag caattttctc
     2161 aaggagttcg ggcagcccgc cgacgtggtc aagatgtccg tgggtgagga gaaccgcgcg
     2221 gacgacgatg ttgttctccc gtgcaaacga gaggatttcc gccgcatcgc cggccggatc
     2281 aaccagaaag ccgtctcccg aagcgtccca tacgagatac gagttcgtcc agagaggccc
     2341 caggggaaat cgtgtgtact tcatcataaa tcccttcctt ttgtacgcat acttcccggc
     2401 gccgcgctat gcggccgcag gttcttttcg cgaagggggg cgcactgcgc gccttcgccc
     2461 tccgcacatg actcagcggt cgatgataag cgtcaccgga ccgtcgttcg caatctctac
     2521 gagcatgtgc gcctggaaaa cgccgctctc cacctcaacg cccctcccgg ccgccgcgct
     2581 cttgaacgat tcgtacattc gattcgcgtg accgggctcg cccgcaccga caaacgaagg
     2641 ccgtcttccc ttccggcagt ctccgtagag cgtgaactgc gagaccgcaa gcatgctgcc
     2701 gccgacttca agcagcgacc tgttcatctt cccctcgtca tcctcgaaga tacggagatt
     2761 gacaatcttc tcggccatcc agtccgcctc ctgttcgccg tcggcgtcgg aaacgccgag
     2821 caggacgcac actcccttcc cgatggctcc ttttgtctct ccgtccacgg aaacccgtgc
     2881 ggaggagact ctttgcacca cagccctcac gacagcgtca cccccgcgat acctggatca
     2941 tgccctttac ggcattgagc ctggcaacta taagatataa gtgttcaagg tcgcggacac
     3001 gaacttcaag cttcattcgc gcctgatttc ctcctacgac tcccgccttg atgcccgtca
     3061 tactgccgtc ccccgcggta attgcctggg cgacgtcgcc gaagagcccg gccctgtcca
     3121 cagcttctat tttcaggcgc gcggagaacc tccgggtacc cgtcattccc cacgacacgc
     3181 tgacgcggcg ttcttcgcgg gagttcctga cgttttcaca gtcggccctg tgcacggtga
     3241 tccccctggt tcgcgtggaa aagccgatga tcgggtctcc gggaaccggc tcgcagcagt
     3301 tggaaaggac gacctggacc ccgtccgccc cctcaaccac gatatcggaa tcccccttct
     3361 tcccggaaaa cgaggtatgc ttctccggtt cggggggaag ggattgcacc ggagcaggct
     3421 gcggctgcgg ctttccggcg aggcgctgcg ccaccagcga gacgctttga tggccgccgc
     3481 ctattgcgga gagcagctct tccccgtttg aataggccgt gtcgcgggca atcttgttga
     3541 gcagcgggag cagttcgtcg aactcttcga actcgggagt ccctctcctc ttgacttcgc
     3601 gttcgagcag atcccggccg cgctgcatct tctccaggcg ctcggtcttt tcgagggcgc
     3661 ggaagtaggc gcgtatcttg ctcctggcct tgctgctgcg ggcgatcttg agccagtctc
     3721 tcgaaggggc tccctgcggc gacgtgatga tcttgacgat atcgccgttc tgcagctcgt
     3781 accccatggc gacgatacgg ttgttcacca tcgcgccgac gcatcgcgtc cccacctggg
     3841 tatggatggc aaaggcgaag tcgagcggcg tcgacccctt cggaagcgaa acgacctcac
     3901 cctgaggcgt gaagacgaag acatccgaag aaagcacgtc ctccttcagg cgctccagga
     3961 attcggacgg ttccgagccg tcatgatccg cctcgatcgc ctgacggatc caggtcagct
     4021 tcgcgtcgag atcgtcgagg gaggcgcctc cctctttgta tcgccagtgc gctgctattc
     4081 cgtactcggc cagggagttc atatcccagg tgcggatctg cacttcaagg ggttctcccg
     4141 tcggtcccac cacggtggtg tggagcgact gg
//
LOCUS       DAOTIL010000135         4164 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminobacteriaceae bacterium isolate AMR_MDS_3026
            NODE_25363_length_4164_cov_2.733755, whole genome shotgun sequence.
ACCESSION   DAOTIL010000135 DAOTIL010000000
VERSION     DAOTIL010000135.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868050
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminobacteriaceae bacterium (wastewater metagenome)
  ORGANISM  Aminobacteriaceae bacterium
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae.
REFERENCE   1  (bases 1 to 4164)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4164)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:19:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,224
            CDSs (total)                      :: 3,198
            Genes (coding)                    :: 3,184
            CDSs (with protein)               :: 3,184
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4164
                     /organism="Aminobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_25363_length_4164_cov_2.733755"
                     /isolate="AMR_MDS_3026"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:3034609"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1317)
                     /locus_tag="P5201_03960"
     CDS             complement(<1..1317)
                     /locus_tag="P5201_03960"
                     /inference="COORDINATES: protein motif:HMM:NF015891.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="secretin N-terminal domain-containing protein"
                     /protein_id="HRV97718.1"
                     /translation="MNRTFRSLMTLSLILLLVSGLPLFAQTPETKPDDDEFALLESAR
                     LMRASGNVQFNFQDVDMVKFVRFMAELLQENIVLAPNIKGAISVISPKPVPLREARQV
                     LLSVLEMNGYTLQPVGDFSKIIPLQAWASAESDVLSGRTAPGEGDQTVSQVVPLNYLT
                     AEFLLPAVLQASGKNVAVQPVGRGNDILLTGRASDVTRAVSVIKALDRPDAIFKSQSV
                     KILHASPELVASHIANLSKVPGSPVFGVAAFPDPVSSQIVLAGEDRELRQALKIVAEL
                     DVVPKSGEFHIYQLQNADAKIVAEQLTKILSTAGADGKVATSIVPDIPSNSIIIAASQ
                     SRFQSIAEIIRTIDTLPRQVLLRGLIAEVNLTKLDNAGIDWATWGGSVAGDAVTAGQI
                     NMGGAVPGTIIDWFQELSKTEEQYRTGTDSNGNPTYGIKTTRKGMGL"
     gene            complement(1314..1703)
                     /locus_tag="P5201_03965"
     CDS             complement(1314..1703)
                     /locus_tag="P5201_03965"
                     /inference="COORDINATES: protein motif:HMM:NF019575.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein"
                     /protein_id="HRV97719.1"
                     /translation="MKKRSAFTLIEVMVAVAVLGLSAAGSLRLLTMSVRALEDVRYER
                     RNLSLARTLWLAAVSDSLDERGREKEYSWETKRFTFERQEEVPEGFYCRQVDLVQGDP
                     MSRPSGSNANAFIFYVPDMKEKKERTK"
     gene            complement(1718..2662)
                     /gene="gspC"
                     /locus_tag="P5201_03970"
     CDS             complement(1718..2662)
                     /gene="gspC"
                     /locus_tag="P5201_03970"
                     /inference="COORDINATES: protein motif:HMM:NF041616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein GspC"
                     /protein_id="HRV97720.1"
                     /translation="MNLHSRSSSTRRISRLLSRQRLERLVALFFRVALVALFFGMGGY
                     WAAAALGEFLSTEEMQSTRSLLSVPLSRSLLPEASERPLTLKEFVGGDPFLVYVPPKP
                     KDESRQITAKELFDLEGIRLTGTLPGISVWIQEAKKPQAVVLKGQKIKGYDLTAVEED
                     RIILQKGPTTLTVLLRYSAETAASAKQSAPAVTAPRAAPQQTLSAARPGQQGSISRET
                     VNQLLMDPLAEMKKFRLRPKFDGDTAAGIEVQWLDDQSILTSLGVKAGDVVQTVNGVP
                     IRNMGDVVNVINSLMSGNTFDVQVVREGKPEMLNYNIK"
     gene            complement(2659..3273)
                     /locus_tag="P5201_03975"
     CDS             complement(2659..3273)
                     /locus_tag="P5201_03975"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRV97721.1"
                     /translation="MKRFASVFGVLLCAFISFVAALYLFFPMDTALELLWRKGMLAAA
                     EKGVTLETAFVGVDEYFPLRIVFRNVRVAAPVASAEAGMLTVRPLFVESILSMSPTAE
                     IRLENFSLGIPLPGQPPIAFSTFYSKSSVRASAIQLSGIQTTGDLVIVGDLTVNPATA
                     KLDEADLHISGERASLLEFAKTMLPLQKDASGAWTLKRKGGENK"
     gene            complement(3270..3815)
                     /gene="gspM"
                     /locus_tag="P5201_03980"
     CDS             complement(3270..3815)
                     /gene="gspM"
                     /locus_tag="P5201_03980"
                     /inference="COORDINATES: protein motif:HMM:NF041618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein GspM"
                     /protein_id="HRV97722.1"
                     /translation="MKLPSFVSNPFDLLGGLDEASWKKKTFYVFLFALLAWGAAFSFW
                     SDGEELRARRTLQRSRFNELVAVLREYSALKSIGGGAKGGEAALAPVAGGDLLSVVSD
                     VVGSLGLRTNMISLSTASVRGGRNGVSISLEGLSAENLAMFLQEMDRRGIETFSADLR
                     AVRENEKQRTLTVFLLLGGAS"
     gene            complement(3812..>4164)
                     /locus_tag="P5201_03985"
     CDS             complement(3812..>4164)
                     /locus_tag="P5201_03985"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRV97723.1"
                     /translation="IFGQNERVVDPLSQARAKLAELRGQGRTENSLSRTLSHLGRTWL
                     DGNKKKAGYPVLEQLRYSADGADITGTAEKMEAIQALRLAADSGGYKASLGDIQQIPG
                     GGLRFTLSLRRDAQ"
BASE COUNT         1023 a         1179 c         1043 g          919 t
ORIGIN      
        1 aagccccata cctttgcgcg tcgttttaat accgtaggtt ggattcccat tagaatccgt
       61 cccggtccgg tactgctcct ccgttttgga gagctcctgg aaccagtcga taatcgtgcc
      121 ggggacggcg cctcccatgt ttatctggcc cgcggtgaca gcgtcgcctg caacagaacc
      181 gccccacgtc gcccagtcga tccccgcgtt gtcaagtttc gtcagattga cctctgcgat
      241 gagcccgcgg agaagcacct gccgcggcag cgtgtcgata gtacgaatga tctcggcgat
      301 cgactggaat cgagactgag aggcagcaat gataatgctg ttggaaggaa tatcaggcac
      361 tatggatgtg gccacttttc cgtccgcgcc cgccgtcgag agaatcttcg tcagctgctc
      421 cgccacaatt ttggcgtcgg cattctggag ctgatagata tgaaactctc ctgatttggg
      481 cacaacatcc agttcggcga cgattttgag cgcttgccgc aactcccgat cttcccctgc
      541 aagaacaatc tgactgctga caggatccgg aaaagctgca acgccgaaca cgggactccc
      601 gggcaccttt gaaagattcg cgatatgcga agcgaccaat tcaggagaag cgtgtagtat
      661 cttcactgat tgagatttaa aaatcgcgtc cggtcggtca agagccttga tcacactcac
      721 agcccttgtt acgtcggaag ctcttccggt aaggaggata tcgtttcccc ggccaaccgg
      781 ctggactgcg acattcttgc ccgaagcctg cagcactgcg ggcaaaagga actccgcagt
      841 cagatagttg agcggcacta cctgagaaac agtctggtcc ccctcgccgg gagcagtgcg
      901 cccgctcagg acatcgctct ctgcggaagc ccacgcctgc aatggaataa tcttgctgaa
      961 gtccccgacc ggctggagcg tatagccgtt catctcgagt accgacagca acacttgacg
     1021 cgcctctctc aagggaaccg gtttgggaga aataacggaa attgcgcctt taatattcgg
     1081 cgcaagcaca atgttctctt gaagcagctc cgccataaaa cgcacaaact tcaccatatc
     1141 aacatcctgg aagttgaact gtacgttccc cgaagcgcgc atcaatcggg cggattcaag
     1201 cagagcaaac tcatcgtcat ccggcttcgt ttcgggagtt tgcgcgaaga gcggcaaccc
     1261 cgaaacaaga agcaaaataa ggctcaatgt catcagcgat cggaaggtgc ggttcatttt
     1321 gttctctcct ttttttcctt catgtcaggc acatagaaga tgaaggcatt tgcgttgctc
     1381 cccgaaggac gcgacatcgg atctccctga acaaggtcga cttggcggca gtaaaaacct
     1441 tcagggacct cctcttggcg ttcaaaagta aagcgttttg tctcccatga atactccttc
     1501 tctcgcccgc gctcatccaa agaatctgaa acggcagcca accagagagt ccgagccaga
     1561 gaaaggttgc gacgctcgta acggacgtct tccagagcac ggacggacat ggtcagcagg
     1621 cgaaggcttc ccgcagcgga gagaccaaga acagcgacgg caaccataac ttcgatgaga
     1681 gtgaaggcgc tccttttttt catcccacac ccgctcctca ttttatatta tagttcaaca
     1741 tttccggctt cccttcccga acaacctgga catcgaaggt attaccgctc ataagggaat
     1801 tgatcacgtt gacgacatct cccatgttcc tgatcggaac gccgttcact gtctgaacca
     1861 catctcccgc cttcactccg agcgaggtaa ggatgctttg atcgtccagc cactgtacct
     1921 ctatgcctgc cgccgtatca ccgtcgaatt taggtcgtaa gcggaatttt ttcatctccg
     1981 ccagcggatc catcagcagc tggttcacag tttccctgga gatagagcct tgttgtccgg
     2041 ggcgtgctgc ggaaagcgtt tgctgcggcg cagctcgcgg cgccgtaacc gcaggtgcgg
     2101 attgtttcgc tgacgccgca gtttcggcgc tataacgcaa aaggacggtc aaggtcgtcg
     2161 ggcctttctg gagaattatg cgatcctcct cgacggcggt caaatcgtac cctttgattt
     2221 tttgtccctt gaggacgacg gcctgaggct tcttcgcctc ctgtatccag acagaaatac
     2281 ccgggagtgt accggtaaga cgaataccct caagatcaaa gagctctttt gcggttatct
     2341 gacggctttc atctttgggt ttgggcggca catacacaag aaaaggatct cccccgacga
     2401 actccttcaa tgtcaatggg cgctctgaag cttccggaag taacgaacgg gagagaggaa
     2461 cgctgagaag cgaccgggta ctctgcatct cctcagtgga taaaaactct cccagcgccg
     2521 ctgcagccca atatccgccc atcccgaaga agagcgccac aagtgcgaca cgaaaaaaaa
     2581 gcgctacgag acgttcgagt cgttgtcggg atagaagacg agaaatacgc cgtgtagatg
     2641 aagagcggga atggagattc acttattttc gcctcctttt cttttaagag tccatgcgcc
     2701 cgacgcgtct ttctgcagcg ggagcattgt cttcgcgaat tcaagcagag aagctctttc
     2761 tccggaaata tgaaggtccg cttcatccag ttttgcggtg gccggattca ctgtcaaatc
     2821 gccaacaatc acgagatccc ccgtagtttg aattcctgaa agctgtatag cggaagcgcg
     2881 aacgctgctc ttcgagtaaa acgtggagaa cgctatcgga ggctgtcccg gaagaggtat
     2941 acccagcgaa aaattctcca ggcgaatttc tgcagtcggc gacattgaca ggatgctctc
     3001 tacgaaaagc ggccgtacgg taagcatacc cgcctccgca gacgctacag gagcagcaac
     3061 acgcacgttg cggaaaacga tgcgcagcgg gaaatattcg tcgactccca cgaacgctgt
     3121 ctccagtgtc acaccctttt cagcagcggc aagcatccct tttcgccaaa gaagttccag
     3181 tgccgtatcc attggaaaga aaaggtacaa ggctgcaaca aaagaaatga acgcgcataa
     3241 aagcactcca aaaaccgacg caaaacgttt catgacgcgc ctcccagcag aagaaaaact
     3301 gtaagcgtcc gttgtttttc attttcccgc acggctcgaa ggtcagcgga gaaagtttct
     3361 attccgcgcc ggtccatttc ctgaagaaac atggcgagat tttcagcgga aagtccttca
     3421 agagaaatgg aaacaccatt acgtcctcct cgaaccgagg cagtgctcaa gctgatcatg
     3481 tttgtacgca gtccaagcga accaactaca tccgacacca cggaaagaag atctcccccg
     3541 gcgactggcg caagagcggc ttctccgcct tttgcaccgc ccccgataga ttttaaagca
     3601 gagtattcgc gtaatactgc gaccagttca ttgaaacggg aacgttgcag cgtacggcga
     3661 gcacgaagct cttctccgtc gctccagaaa gagaacgcag ctccccacgc taagagagca
     3721 aaaagaaaaa cgtaaaaagt ctttttcttc caggatgctt cgtcaagtcc tccgagcaaa
     3781 tcgaaaggat tcgaaacaaa cgaggggagt ttcattgagc atccctcctc aatgagagtg
     3841 tgaatcgaag tcctccgccc ggtatctgct gaatatcgcc caaggaagcc ttatagcctc
     3901 cactgtcggc agcgagccga agcgcttgaa tcgcctccat cttctccgcc gtacctgtga
     3961 tatccgcacc gtcagcagag tagcgaagct gttcaagcac cgggtaccca gctttctttt
     4021 tattcccgtc aagccatgtc cttcctagat gtgagagcgt acgcgacaat gaattctccg
     4081 ttcttccctg gccccgaagt tccgcgagct ttgctctggc ttgagaaagc gggtcgacaa
     4141 ctcgttcgtt ctgcccgaat attt
//
LOCUS       DAOTIL010000136         4162 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminobacteriaceae bacterium isolate AMR_MDS_3026
            NODE_25376_length_4162_cov_4.120769, whole genome shotgun sequence.
ACCESSION   DAOTIL010000136 DAOTIL010000000
VERSION     DAOTIL010000136.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868050
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminobacteriaceae bacterium (wastewater metagenome)
  ORGANISM  Aminobacteriaceae bacterium
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae.
REFERENCE   1  (bases 1 to 4162)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4162)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:19:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,224
            CDSs (total)                      :: 3,198
            Genes (coding)                    :: 3,184
            CDSs (with protein)               :: 3,184
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4162
                     /organism="Aminobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_25376_length_4162_cov_4.120769"
                     /isolate="AMR_MDS_3026"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:3034609"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1402)
                     /gene="hypF"
                     /locus_tag="P5201_03990"
     CDS             complement(<1..1402)
                     /gene="hypF"
                     /locus_tag="P5201_03990"
                     /EC_number="6.1.2.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003998 - acylphosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyltransferase HypF"
                     /protein_id="HRV97724.1"
                     /translation="MLFQTRILVSGIVQGVGFRPFCVRLANSESISGYALNTSHGVEM
                     ELHGEEENLDSFLFRIVRENPPASFISSVAILDEKRKSSSPPARFEIMESRKETLQTA
                     LIPADLAVCAQCLAEMKDPENRRYRYPFINCTDCGPRYTIIRELPYDRPKTTMASFVM
                     CPKCSSEYADLEHRRFHAEPNACPLCGPEVWLTDSNGTELTRGDEAVRLCSSLLDQGK
                     IVAIKGIGGFHIACTPFSDETVALLRDRKRRAHKPFALMVSSLEIAEELTYLPAVAKR
                     LMESPASPVVLCCVRPKPRSSDVAAVSPLVAPGQRTLGLMLPYTPVHHLLLEGKRALI
                     MTSANFSDAPIVSDNAAALASLSSIADFFLFSNRDIEMPIDDSVAMPFGRSFFLLRRA
                     RGYTPLPMNFRRGDSCVILGAGAAMKGRFCLSRDDVLLPGQYLGDMKQRDTVKYYNRA
                     LEHFVKLYELKPGKVAF"
     gene            1475..2002
                     /locus_tag="P5201_03995"
     CDS             1475..2002
                     /locus_tag="P5201_03995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014806701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cob(I)yrinic acid a,c-diamide
                     adenosyltransferase"
                     /protein_id="HRV97725.1"
                     /translation="MPILEYGLVHVYMGHGKGKTTSALGIVLRMAGCGGKSAVVQFMK
                     GWPYSEAKGLQFLPGVTLFQTGRADYVYPDKIDPLDYSEAERGLSIGAEAVFSGEYDL
                     VVLDELNVALSFGLVPLDRVLALIGRKPPHVELVFTGRNPPQELIDRADLITEMIEIR
                     HPYQKGILARKGIDC"
     gene            2096..>4162
                     /locus_tag="P5201_04000"
     CDS             2096..>4162
                     /locus_tag="P5201_04000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006301143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HRV97726.1"
                     /translation="MTKATTNPSRKGSKITAGSAQNKSLTIEQLRKKTDAASLGISTT
                     NEIAPLEEFLGQKRAVSSISFGLEVESKGYNIVVLGNPGSGRTTYALERLRTAARKRT
                     PPDDWVYVYNFEDPGQPLAINLPAGKGKEMGTAFDEMVEELKTSISKAFEKSQYEDAK
                     AQLVKEFQEEVNQLMDEVKAWAAENGFSLKRTPQGFVNIPLTEEETESGEKTKREIQQ
                     EEFEALSEEEQKSLQKKSEEVSQKTLETLRKIRDLEKGLKERIVKLEAEICRNAIMPY
                     LSDIKEKFGTNGDSSKDENQKSNDVLGSWIDALAEDIIDNFGMFVAAVKDENAEIDFT
                     RYTVNVFVCNDPENGAPVIWETNPTYYNLGGKVEYESRQGYLYTDFKKIVPGAFQKAN
                     GGFLVLDAEKVLLNFMSWEALKRILRTGEATIENLGEQYGAVPVSSLRPQPIPINMKV
                     VMVGTPFLHALLQYYDPEFQKMFKIKADFDTDMERTPETEKQMAQFIASCLKKENGLP
                     FDASGVAEVIDCSARIADDQGRISTEFNKITEIIVESTAWAKAENADTVQREHVKKAI
                     REKRFRSDLIQEKIARAYQDGLVRIDTSGKVVGQINGLSVIDLMDYRFGHPSRITANV
                     FMGQDGVVNIEREVKMTGPIHNKGLLILTSYMGRKYAQDMPLSLSAKLTFEQMYGGIE
                     GDSASST"
BASE COUNT         1136 a         1056 c         1119 g          851 t
ORIGIN      
        1 cgaaagcgac cttccctggt ttcagctcgt agagtttcac gaagtgctcc agcgccctgt
       61 tgtagtactt cacggtgtcg cgctgtttca tgtcgccaag atactgcccg gggagcagca
      121 cgtcatcccg ggataaacag aatctccctt tcatcgcggc gcccgcaccc aagataacgc
      181 agctgtctcc acggcggaaa ttcatcggaa gcggagtgta ccctctggcg cgccgcagga
      241 ggaaaaatga acgtccaaac ggcatcgcaa cggaatcgtc gatgggcatc tcgatatcgc
      301 gattcgaaaa aaggaaaaag tccgcgattg aggagagcga ggcaagagcc gcagcattgt
      361 cggagacgat cggggcgtcc gaaaagttcg cgctggtcat gataagcgcc ctctttcctt
      421 cgagaagcag atgatgcacc ggcgtgtacg ggagcattaa tccaagagtc cgctgcccgg
      481 gagcaacaag aggagataca gcggcgacat cggaggaacg cggcttcggg cgaacgcagc
      541 agagcaccac aggacttgcg ggagattcca tgagtctctt tgcgacagcg ggcagatagg
      601 taagctcctc tgcaatttca agagaagaaa ccatcagcgc aaacggttta tgcgctcttc
      661 gcttccggtc tcgcaacaag gcaactgtct cgtcactgaa aggggtacac gcaatatgaa
      721 aaccgcctat accttttatt gcaacgattt tgccctggtc aagcagcgag gaacaaagtc
      781 ggacagcttc gtcccccctt gtcagctccg ttccattcga atccgtaagc cagacctccg
      841 gaccgcagag ggggcacgca ttcggctcgg catgaaaacg cctgtgctcc aaatccgcat
      901 actcactgct gcacttgggg cacataacaa acgaagccat tgtggtcttc ggacgatcat
      961 agggaagttc gcgtataata gtgtaacggg gaccacagtc ggtacagttg ataaaagggt
     1021 agcgataccg gcggttttcc ggatccttca tctcggcaag acactgtgcg cacacagcaa
     1081 gatcggccgg aatcagggcg gtctgcagtg tttcttttcg gctctccatt atctcaaaac
     1141 gagcgggcgg actcgaagac ttccgtttct cgtcaaggat tgcaacggag ctgatgaagg
     1201 acgccggagg gttttcccga acgattctga agaggaagct gtcaagattc tcttcctcgc
     1261 catgcagttc catctcgacg ccgtgcgagg tattcagcgc ataccctgaa atactctcgg
     1321 aattcgcaag acgaacacaa aaggggcgaa accctacgcc ctggactata cctgaaacaa
     1381 gaattcgcgt ttgaaaaagc attgacaaac ctccgttctg aaggcattgt aatgcatatg
     1441 gaggggaaat gcatagatgg acgggggaga tacaatgccg atacttgaat acggtttggt
     1501 tcacgtgtat atgggacacg gcaaagggaa gaccacaagc gcgctcggta tcgtgcttcg
     1561 aatggctgga tgcggaggga aatccgctgt cgtccagttc atgaaaggct ggccgtacag
     1621 cgaagcaaaa gggcttcagt ttctccccgg agtgacgctc ttccagacgg ggagagcaga
     1681 ttacgtgtat ccggacaaga tagatccttt ggattacagc gaggcggagc gcggcctttc
     1741 catcggcgcc gaagcggtgt tttcggggga gtatgacctc gtggttcttg acgaactgaa
     1801 tgtcgcgctt tcgttcgggc ttgtcccgct agaccgggtg ctagctctta ttggacgtaa
     1861 accgcctcat gtggagctcg tttttacggg gagaaatcct ccgcaggaac tcattgaccg
     1921 agccgatctc attacagaaa tgattgaaat ccggcaccct tatcaaaaag gtattcttgc
     1981 ccgcaagggg atcgattgct gagaagtttc ttgcccttct cacgaaaacg aaacagatct
     2041 gttctctcgt gaatgcatac aaacaatgaa attgacatct ctggaggtag catctatgac
     2101 aaaagccacg acgaacccct cccgcaaggg atcaaaaata acagcaggga gcgctcaaaa
     2161 caaatcgctc acaattgagc agcttcggaa aaaaaccgat gccgcctcgc tcggcatttc
     2221 aacgacgaac gaaattgcac ctcttgagga gttccttggt cagaaacggg ctgtatcctc
     2281 gatctccttc ggcctcgaag ttgagagcaa gggatacaac atcgtcgtgc tcgggaatcc
     2341 cggcagcggt agaacaacgt atgctcttga acgcctccgc acagcagctc gcaaacgcac
     2401 tcctcccgac gactgggtgt atgtatataa ctttgaggac ccggggcagc cacttgctat
     2461 caacctcccg gcaggcaaag ggaaagaaat gggtacggcg ttcgacgaaa tggtcgaaga
     2521 gctgaagaca tcgataagta aggcgttcga aaaaagtcag tacgaagacg ccaaagcaca
     2581 gctcgtcaaa gagtttcagg aagaagtcaa tcaactcatg gatgaagtca aggcatgggc
     2641 tgctgaaaac ggcttttcct tgaagaggac cccgcagggt tttgttaata tccccttgac
     2701 ggaagaagaa acggagagcg gagaaaaaac aaagcgggag attcagcagg aagaatttga
     2761 ggcgctctcc gaggaggaac agaaaagtct ccagaagaaa tcggaggagg tttcccaaaa
     2821 gacgcttgaa acgctccgaa agatccgcga ccttgaaaaa ggactgaagg aacgcattgt
     2881 caaactagag gctgaaatat gccgcaatgc aatcatgccc tatctttccg acatcaaaga
     2941 aaagtttgga acgaacgggg attcctccaa ggatgagaat caaaagagca acgacgttct
     3001 tggaagctgg atcgacgccc ttgccgaaga tatcattgac aacttcggca tgtttgtggc
     3061 ggcggtcaaa gatgaaaacg ccgagatcga ttttacccgg tacacagtaa acgtttttgt
     3121 ctgcaacgat cccgagaacg gcgctcccgt tatttgggag acaaacccta catactacaa
     3181 tttgggcgga aaggttgaat acgaaagccg ccaagggtat ctttataccg acttcaaaaa
     3241 aattgtcccc ggagcatttc agaaggccaa cggaggattc ctcgtccttg acgccgagaa
     3301 agtactgctc aatttcatgt cgtgggaagc gctgaagcgc attctccgca ccggcgaagc
     3361 gaccatagaa aacctgggag agcagtacgg cgccgttccc gtctcttccc tgcgccctca
     3421 acccataccg ataaacatga aggtcgtgat ggtgggaacg ccttttcttc atgcgctgct
     3481 tcagtattac gacccggagt ttcagaagat gttcaagatc aaggcggatt tcgacaccga
     3541 catggagcga acgcctgaga cagaaaaaca gatggcccag ttcatcgctt cgtgccttaa
     3601 aaaagaaaac gggcttcctt tcgacgcatc cggagtcgcc gaggtgatag actgttcggc
     3661 cagaatcgcc gacgatcaag ggcgaatctc cacggagttc aacaaaatta cggagatcat
     3721 cgtcgagtcc acagcctggg ctaaagcgga aaatgccgat actgttcaac gtgaacacgt
     3781 gaagaaggct attcgagaaa agcgcttccg ttcggattta atccaagaga agatagcacg
     3841 cgcctaccag gatggtctgg tcaggatcga cacttcggga aaagtagtcg gccagatcaa
     3901 cggcctttca gtcatcgacc tgatggacta tcgtttcgga cacccctcca gaatcaccgc
     3961 caacgtcttt atgggacagg acggggtcgt caatatcgag agagaagtca agatgacggg
     4021 acctatccac aataaggggc tcttgattct gaccagctac atggggcgaa agtacgcgca
     4081 ggatatgccg ctttcactct ccgctaaact gacattcgaa cagatgtacg gcgggatcga
     4141 aggagacagc gcttcctcga cg
//
LOCUS       DAOTIL010000137         4159 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminobacteriaceae bacterium isolate AMR_MDS_3026
            NODE_25396_length_4159_cov_3.577242, whole genome shotgun sequence.
ACCESSION   DAOTIL010000137 DAOTIL010000000
VERSION     DAOTIL010000137.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868050
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminobacteriaceae bacterium (wastewater metagenome)
  ORGANISM  Aminobacteriaceae bacterium
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae.
REFERENCE   1  (bases 1 to 4159)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4159)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:19:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,224
            CDSs (total)                      :: 3,198
            Genes (coding)                    :: 3,184
            CDSs (with protein)               :: 3,184
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4159
                     /organism="Aminobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_25396_length_4159_cov_3.577242"
                     /isolate="AMR_MDS_3026"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:3034609"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..881)
                     /locus_tag="P5201_04005"
     CDS             complement(<1..881)
                     /locus_tag="P5201_04005"
                     /inference="COORDINATES: protein motif:HMM:NF012707.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HRV97727.1"
                     /translation="MRRERVIKQLGRSLPAFSAAALLFLCIAVCAACLFAFPSDASLM
                     EDFRSSGEAVELSGEERDYLAALGPVTVCPDPDWMPFEHVDEQGNFTGIAADLLELIA
                     GRLGIEFSYVFPKDWSEAVALSKSGDVLILPFLNKTPAREEWLLFTEPLLVDPNVFIA
                     REEHPYIYDASQLTDRTIVLPEGTSMEERLRRDFPNLDVITVEREEDVFRAVVERRAD
                     MTLRSLTIAAWTIRKEGYFNLKVVGQAPEEYVNRLRMGVLKSEPRLRDILNKGIAAIT
                     PSELEAIVNRHVNITVVR"
     gene            complement(881..3085)
                     /locus_tag="P5201_04010"
     CDS             complement(881..3085)
                     /locus_tag="P5201_04010"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HRV97728.1"
                     /translation="MTKRKQADENCPGSAGLAAGSCGEETLQEENRRLKLEAAVAREA
                     ASKAMAASIAKSEFLANMSHEIRNPMNGVIGMTGLLLDTRLNGEQRYYAGMVRDSAVS
                     LLSILNDILDFSKIEAGKLELETLDFDLPSLLQDFSATMSLRAREKNVALLCSIAPDV
                     PSLLRGDPGRLRQILNNLVGNALKFTQAGEVELGVFLEEESENFALLRFSVRDTGIGI
                     PEERLPGIFDKFMQVESSTARTFGGTGLGLAISKQLAEMMGGRIWATSVEGKGSEFCF
                     TAALGKQYEQSVPDISSVDVRGVRVLVVDEKPGSFKLLTTRLLSWGMRPSEVSDGASA
                     ISLLREAVRAKDPFLLAVMDTQTGDMDGETLGRLIRGDELLSATRTVMLASIGVRGDA
                     KRFADAGFSGYLSGPALQNDLHDIFSLVLSEQRKNTRFIATRHTVREALPPFDGKGAH
                     VLLAEDNVTNQRVAIGMLKKFGVTVDVVADGLDALRLLRKGGYDLVLMDCQMPKMDGF
                     ETARRIRSMEGPRRDVPIVAMTAYAMVGDREKCLQAGMNDYIAKPILPALLVDTLKKW
                     LPEREKERTSPEKEGGPPVAKPKKTNAPKQKKTALRVPAAGAPAAPEQQAWNRGLLLE
                     RLMGDEELVMEVLQDFLSELPRELRKLGDVLSSDNLPLMERHAHSIKGMSACTGAEAL
                     ASVAFKIEERAREEDAEGARSFLPALKKEWKRLKTELEEERAPRNHSRRTRT"
     gene            complement(3316..3525)
                     /locus_tag="P5201_04015"
     CDS             complement(3316..3525)
                     /locus_tag="P5201_04015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014163740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dodecin family protein"
                     /protein_id="HRV97729.1"
                     /translation="MESVYKVIELVGTSSESWEKAAAAAVELASKSLRDLRIAEVTAM
                     DMHLEDGKIMAYRTKLKVSFKFEDK"
     gene            3760..>4159
                     /locus_tag="P5201_04020"
     CDS             3760..>4159
                     /locus_tag="P5201_04020"
                     /inference="COORDINATES: protein motif:HMM:NF014614.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cob(I)yrinic acid a,c-diamide
                     adenosyltransferase"
                     /protein_id="HRV97730.1"
                     /translation="MESVGCVHVYTGTGKGKTTAALGLMLRAAGAGLSVLLVQFLKRA
                     EYSEHEALKLLGDRVTVRTFGGERRIGAPATDEDRKLAREGLAFLGTALASGAYDLVV
                     ADEIVVAAHIGLLLKEEILAQPRFHTGLAAL"
BASE COUNT          798 a         1333 c         1169 g          859 t
ORIGIN      
        1 cggacgaccg tgatgttcac atgtcggttg acgatcgcct ccagctcgct cggcgtgatc
       61 gcggcaattc ctttgttcag gatgtcgcgc aaccgcggct cgctcttcag gacgcccatt
      121 cggagacggt tgacatactc ctcgggagcc tgcccgacaa ccttcagatt gaagtagcct
      181 tccttgcgga tggtccaggc ggcgatagtc agcgaacgga gcgtcatgtc cgcccgtcgc
      241 tcgacgaccg cgcgaaaaac atcctcttcg cgttcgaccg tgatcacgtc caggttggga
      301 aagtcgcggc ggagacgctc ctccatcgag gttccctccg gcaggacgat cgtcctgtcc
      361 gtgagttgcg aagcgtcgta gatatagggg tgttcttcgc gggcgataaa gacgttcggg
      421 tccaccagca gcggttcggt gaagagaagc cattcctccc ttgcgggagt cttgttcagg
      481 aagggaagaa tcagaacgtc gcccgacttg gagagcgcca cggcctcgct ccagtccttc
      541 gggaaaacgt aggagaattc aatgccgagg cgtccggcga taagctccag cagatccgcc
      601 gctattcccg tgaagtttcc ctgttcgtcg acgtgctcga acggcatcca gtccggatcg
      661 ggacagactg tgacgggacc gagcgccgcc agatagtccc gctcttcccc ggagagttcg
      721 acagcctccc ctgacgagcg gaagtcctcc atgagcgacg catccgaggg aaaagcgaag
      781 agacaggctg cgcacacggc gatgcacagg aaaagaagcg cagcggcaga gaacgccgga
      841 agagaacgcc caagttgctt gatgacgcgc tcccgtcgca ttacgttctc gtcctcctgc
      901 tgtgattgcg cggcgctcgt tcttcttcca gctcggtttt cagccgcttc cactcctttt
      961 ttaacgcggg aaggaagctt cgcgcaccct ccgcgtcttc ttctctcgcc cgctcctcta
     1021 tcttgaaagc aacggaggcg agagcctcgg ccccggtgca tgcgctcatc cccttgatac
     1081 tgtgcgcatg ccgctccatc aaggggaggt tgtcagagga gagcacatcc ccaagcttcc
     1141 ggagttcgcg gggaagctcc gacaggaaat cctgaagcac ttccattaca agctcctcgt
     1201 ctcccatcag gcgctccagg agcagccccc tgttccaggc ttgctgctcc ggtgcagcgg
     1261 gcgcgccggc cgccggaacg cgaagagctg tcttcttttg cttcggcgcg tttgtcttct
     1321 tcggcttcgc gaccggaggc ccgccctctt tctccggaga cgttcgttct ttttcacgtt
     1381 caggaagcca ttttttaagc gtatcgacca gaagcgccgg aagtatcggc ttcgcgatat
     1441 agtcgttcat cccggcctga agacacttct cccggtcgcc gaccattgca tacgccgtca
     1501 tggcgacaat cggcacatcg cggcgcggtc cctccatgct ccggattctg cgcgccgtct
     1561 cgaagccgtc catcttcggc atctggcagt ccatcagcac gaggtcgtag ccgccttttc
     1621 gcagcaaacg cagcgcgtcg aggccgtcgg caacgacatc gaccgtcact ccgaattttt
     1681 tgagcatgcc tatcgccaca cgctggttgg taacgttgtc ctccgccaga agaacatgcg
     1741 cgccctttcc gtcgaaaggc ggaagcgcct cgcgcacggt atggcgagtg gcgatgaaac
     1801 gcgtattctt tcgctgttcc gacaggacaa gcgaaaagat atcgtgaagg tcgttctgga
     1861 gcgccgggcc gctcaggtaa ccggagaatc ccgcatccgc aaagcgtttc gcgtcgccgc
     1921 ggacgcctat ggaagccagc atgacggtgc gcgtggccga gagcagttca tcgcccctga
     1981 tgaggcgtcc caacgtctcg ccgtccatat cgcccgtctg cgtatccatg accgcaagga
     2041 gaaagggatc ctttgcccgc accgcttcgc gcagaagcga gatggcggac gcgccgtccg
     2101 aaacttccga ggggcgcatt ccccaagaga gcaggcgggt cgtcagaagc ttgaaactcc
     2161 cgggtttttc atccacgacg agcacgcgca cgccgcggac atccaccgag gagatatccg
     2221 gaaccgactg ttcatactgt tttcccagcg cggcggtgaa acaaaattcc gaacccttcc
     2281 cctcgacgct cgtcgcccag atccgtccgc ccatcatctc cgcaagctgc ttcgaaatgg
     2341 caagcccgag acctgtcccc ccgaatgtcc gtgccgtgga cgattccacc tgcatgaact
     2401 tgtcgaaaat gccggggagc cgctcctccg gaatgccgat tcccgtatcg cgcaccgaga
     2461 agcgaaggag cgcgaagttc tcgctttcct cttcaaggaa aactccgagc tcaacctcgc
     2521 ccgcctgagt gaacttcaag gcgttcccca caaggttgtt caatatctgg cgaagccgcc
     2581 cgggatctcc gcgaagcaac gagggaacgt cgggagcaat gctgcacaga agcgcgacgt
     2641 tcttttctct ggcgcgaagc gacatcgtcg cggaaaagtc ttgaagcaga cttggaagat
     2701 cgaaatccag agtctccagc tcaagtttcc ccgcttcgat tttggagaag tcaaggatat
     2761 cgttcaggat gctcaacaac gagaccgcgc tgtcgcggac catcccggca tagtagcgct
     2821 gttcgccgtt caggcgcgtg tccagcaaga gcccggtcat gccgatgacg ccgttcatag
     2881 ggttccggat ctcatggctc atattggcaa gaaattcgct cttcgcgatg ctcgccgcca
     2941 ttgccttcga agcggcctcc cgcgccaccg cagcttcgag cttcaagcgg cggttctcct
     3001 cttggagcgt ctcttctccg caactccccg cggcgaggcc cgcactcccc gggcagttct
     3061 cgtcagcctg ttttctcttc gtcatgtcgt cgctcccgtg acgtgtaagt ttcgctctcc
     3121 gagcgatccg gcccgctctc cccggatttg ataaaccagc gtctccgccg ttcatacaac
     3181 gcgcttcgtg cactcggttc gattatatca tccgtgcttt tcaggctcta tgggcggtca
     3241 tcgggaatac gaagccgccc gcttaaagaa ctttatcggg cgggcggctt cctggtacgg
     3301 caacgcaggg gcatgctact tatcttcgaa cttgaacgat actttgagct tggtccggta
     3361 cgccataatc ttgccgtcct cgaggtgcat atccatggcg gtaacttccg cgatacgcag
     3421 gtccctcaac gacttcgacg ccagctccac tgccgccgct gcggccttct cccacgactc
     3481 gctgctcgtc cccacaagct cgatcacctt gtacacgctc tccatgtcat ctcctccttc
     3541 cagatcggct ccgaagagcc cgccagtttc cgccggcaaa gcatcgccgc cagaaaaaag
     3601 aatagtttaa ttataccatt accactcttc atttcactct gaataaatac ttttcgggac
     3661 gcttgcttct gcacgggctg cgccgttatc cgcatccccc cggcgaacgt ccttttccat
     3721 accgtataat gagagaaaaa gcagggaggg gcttcgttta tggagagcgt cggttgcgtg
     3781 catgtctaca ctggaacggg aaagggaaag accacagcgg cgctcggcct gatgcttcgc
     3841 gctgccgggg cggggctttc cgtgctcctc gtccagttcc tcaaaagggc ggaatacagc
     3901 gaacacgagg cgctgaagct cctcggggac cgcgtgaccg tccgcacctt cggaggagag
     3961 cggcggatcg gcgctcccgc aacagatgag gaccggaagc tcgcgcggga ggggctggcc
     4021 tttctcggga cggcgctcgc gtccggagcg tacgatctcg tcgtcgccga cgaaatcgtg
     4081 gtggcggcgc acatcgggct gctgctcaag gaggagattc tcgcccagcc acgcttccat
     4141 accggcctgg ccgccttgg
//
LOCUS       DAOTIL010000138         4155 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminobacteriaceae bacterium isolate AMR_MDS_3026
            NODE_25418_length_4155_cov_3.647805, whole genome shotgun sequence.
ACCESSION   DAOTIL010000138 DAOTIL010000000
VERSION     DAOTIL010000138.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868050
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminobacteriaceae bacterium (wastewater metagenome)
  ORGANISM  Aminobacteriaceae bacterium
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae.
REFERENCE   1  (bases 1 to 4155)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4155)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:19:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,224
            CDSs (total)                      :: 3,198
            Genes (coding)                    :: 3,184
            CDSs (with protein)               :: 3,184
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4155
                     /organism="Aminobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_25418_length_4155_cov_3.647805"
                     /isolate="AMR_MDS_3026"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:3034609"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..415
                     /locus_tag="P5201_04025"
     CDS             <1..415
                     /locus_tag="P5201_04025"
                     /inference="COORDINATES: protein motif:HMM:NF014821.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="Ada metal-binding domain-containing protein"
                     /protein_id="HRV97731.1"
                     /translation="TTVLKVAHHGSHNGTDARFLAMLRPKTAIISCGAENPYGHPHRE
                     AVALLEEAGVDLFTTIGGDVVVETDGIEFTVRKVLESEKSGQEKVRFYIGNRSSRVFH
                     TSACDGLPSERNRVYFKSAEEAVKAGYRPCGRCVQ"
     gene            412..627
                     /locus_tag="P5201_04030"
     CDS             412..627
                     /locus_tag="P5201_04030"
                     /inference="COORDINATES: protein motif:HMM:NF022659.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3006 domain-containing protein"
                     /protein_id="HRV97732.1"
                     /translation="MRAFIDSVHEGVARVLLGDDESLAVEMPVSWLPEGAAEGKALRV
                     LWELDEEETFCARKAVEDLYDELGDMP"
     gene            complement(704..2479)
                     /locus_tag="P5201_04035"
     CDS             complement(704..2479)
                     /locus_tag="P5201_04035"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRV97733.1"
                     /translation="MKKHVLRIMLAALTLLLVSSACWADMGEIQNFTFRGVGEDVLGP
                     GEDPTPDGKQDYFFSVTVSGIGALSGFILQNEDGNSVWDTVAGNNVPGMTVKDSSGNI
                     IVHSGGSMPLVPLILGAGFTISVHDDGSIARGGKFALVARFADGSRSQSVIEIPASEQ
                     PADAAASVKILSAEWTGTGTRDLTRGDEQLRGDGKADMTARISVQGKGVLTGITIRSV
                     EGEKGAWDTVPANRAPLIAVTQGNKTLNAADGNIEIPLDGKYTFDLLLTDTGAIARGK
                     SRFEVVLVFSDGSVVAKEIAKGKSEEPSSGEFSGSAVFAGPGNRDLVGRNEQRRGNGS
                     PDWKIDLKIETLGTISGIKVFNSTGPAGEWDTIPGNGRWLVAVTGADGKLLNTADGSV
                     RIAVPNPLALQLWVEDNGSLEDPNVRSKVTLFYDDGRELTREIVSSGSKEDETPTPTP
                     RVRPDRRRERVKREIFFSNPRQASSSDYVGSKDKIGKSGKKDWLFEFRIEGNGAVKQI
                     TVEAVDASFIWDTVPKNRNWVIGVLAPGKGLLNKKDGSVSFEVPPHNNLQLFVEDNGV
                     LKQGASQFEITVTWSDGATSKVSNF"
     gene            complement(2517..3002)
                     /locus_tag="P5201_04040"
     CDS             complement(2517..3002)
                     /locus_tag="P5201_04040"
                     /inference="COORDINATES: protein motif:HMM:TIGR01909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C-GCAxxG-C-C family protein"
                     /protein_id="HRV97734.1"
                     /translation="MDETTRGQKTASGIGRTAADSFLSGLYCAESALLAVAESRGTRS
                     ELIPNIATGFCSGMARTCGTCGALTGAIMGIGLALGRSAAAESVEPAYRAVRQLIAEF
                     EEEFGARDCHLLLGCDLGTPEGQAVFREKGLRDRCAEYTGKAAEIAARVIAEIEEERR
                     P"
     gene            3234..3584
                     /locus_tag="P5201_04045"
     CDS             3234..3584
                     /locus_tag="P5201_04045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005661144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043856 - anti-sigma factor antagonist
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="STAS domain-containing protein"
                     /protein_id="HRV97735.1"
                     /translation="MFEMHFTVETWGRSVLLSGRLDARSAPDFEKAALAAADEQPEKR
                     LHMLLDFSGLEYISSAGLRSILVLAKRLSPSGGKVVLCGMSGVVEEVFGVSGFDSFLP
                     VTKSREEALRLLGE"
     gene            3599..4027
                     /locus_tag="P5201_04050"
     CDS             3599..4027
                     /locus_tag="P5201_04050"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005661145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HRV97736.1"
                     /translation="MENTVRERVFPASLDLLGDIRAFILEEDGAEERLPPKRVAHLDV
                     AIEEIVVNICSYAYETPPGELTIRVEEKPEFFAVDFLDNGVAFDPLAMDEPDVSLPLE
                     KRDAGGLGILLVRRMMDEVHYSRKGTLNSLRIVVDTPRMP"
BASE COUNT          764 a         1239 c         1237 g          915 t
ORIGIN      
        1 gacgacggta cttaaggtcg cgcaccacgg ctcgcacaac ggcacggacg cgcgattcct
       61 cgcgatgctc cgcccgaaga cggccatcat ctcctgcggc gcggagaacc cgtacggaca
      121 cccgcaccgg gaagccgtgg cgctgttgga agaggccggc gtcgacctgt tcacgacgat
      181 cgggggcgac gttgtcgtcg agacggatgg aatcgagttt accgttcgga aagtccttga
      241 atcggagaag tccggtcagg agaaagtgcg tttctatata ggaaaccgtt ccagccgggt
      301 gttccataca tccgcgtgcg acggtctccc ttccgagaga aaccgtgtgt acttcaaaag
      361 cgcggaagaa gctgtaaaag ccggataccg gccgtgcggg aggtgcgttc agtgagggcg
      421 ttcatcgatt cggtgcatga gggggtagcg cgcgttctgc tcggcgacga cgagtcgctc
      481 gcggtggaga tgcccgtctc ctggctgccc gagggggcgg ccgaggggaa ggcgctccgg
      541 gtgctgtggg agcttgacga agaagagacg ttctgcgcgc ggaaggccgt ggaggacctc
      601 tacgacgagc ttggcgacat gccgtgagga aatgcgctga acggatggga gagctacaga
      661 aaaagggaga aggcgctcgt ccttctccct ttgttaccac agattaaaaa ttggatactt
      721 ttgaagtggc gccgtcgctc caggtcaccg tgatttcaaa ctgcgacgct ccctgtttca
      781 acaccccgtt gtcctccacg aaaagctgaa ggttgttgtg cgggggaacc tcgaaggaaa
      841 cagatccgtc cttcttgttc aacagcccct tccccggagc cagtacgccg ataacccagt
      901 tcctgttttt cgggacggtg tcccagatga acgaagcgtc cacggcctct accgtgatct
      961 gcttgacggc gccgtttccc tcgattctga attcgaagag ccagtctttc ttgccgctct
     1021 tacctatctt gtccttcgat ccgacgtagt cgctgcttga ggcttggcgg ggattcgaga
     1081 aaaagatctc ccgcttcact ctctcacgcc gtctgtcggg gcgcactctc ggagtgggcg
     1141 tcggcgtctc atcctccttc gagccggagg agacaatttc tctggtgagc tctcttccgt
     1201 cgtcatagaa cagtgtgact tttgagcgca cgttcggatc ttcgagggaa ccgttgtcct
     1261 ctacccagag ttggagcgcc agggggttcg gaacggctat ccggacggag ccgtccgcgg
     1321 tgttcaggag ttttccgtcc gctcctgtga cggcgacgag ccagcggccg tttccgggga
     1381 tcgtgtccca ctcgcccgca ggtccggtcg agttaaaaac ttttatcccg ctgatcgtcc
     1441 cgagcgtctc gatcttgagg tcgatcttcc agtcggggct tccgttgccc ctgcgctgct
     1501 cgtttctccc tacaagatct ctgtttcccg gaccggcaaa aacggcggaa cccgagaact
     1561 cgccggaaga tggctcctcg ctctttccct ttgcaatctc tttggcgacg acagatccgt
     1621 ccgagaagac gaggacgact tcaaagcgcg acttcccgcg ggcgatcgct cccgtgtccg
     1681 tcagcagcag gtcgaaagtg tatttgccgt cgagaggaat ttcgatgttt ccgtccgctg
     1741 cgttcagcgt cttgttcccc tgcgttactg cgataagagg cgcgcggttg gcaggtacgg
     1801 tatcccaggc gcccttctcc ccttcgacgc tccggatggt gatgccggtg agcacgccct
     1861 tgccctggac ggaaattcgg gccgtcatgt cagcctttcc gtcgccgcgg agctgttcgt
     1921 ctccgcgggt aagatcgcgc gtccccgttc ccgtccattc agcggagagt atcttgaccg
     1981 aagccgccgc gtccgccggt tgctcggaag cgggaatctc gatgacggac tgactccgcg
     2041 agccgtcggc gaatcgagcc accagggcga actttccgcc gcgcgcaata gatccgtcgt
     2101 cgtgcacgga gatcgtgaat cccgccccga ggatcaacgg aaccaacggc atgctgccgc
     2161 cgctgtggac gattatattg ccgctgctgt ccttcaccgt cattccgggg acgttgttcc
     2221 cggcgacggt atcccacacg gagttcccgt cctcgttttg cagtatgaac ccggaaaggg
     2281 cgcctatccc tgaaacggtc accgaaaaga aataatcctg tttcccgtcc ggcgtagggt
     2341 cttcgccggg gccgagcaca tcctcaccga cacctctgaa cgtgaagttc tgaatttctc
     2401 ccatgtcagc ccagcaggcg gacgaaacca gcaaaagtgt caatgccgcg agcatgatgc
     2461 gcagaacatg ttttttcacc tgaaccatct cctttgtagg tatttccccg ttattgttat
     2521 gggcggcgct cttcctcgat ttccgctatg acgcgcgccg caatctccgc ggctttcccc
     2581 gtgtactcgg cgcagcgatc gcgaagcccc ttctcccgaa aaacagcctg cccctccggc
     2641 gtcccaaggt cgcacccgag cagcaggtga cagtcgcgcg cgccgaactc ctcttcgaac
     2701 tcggcaatga gctgcctgac cgccctgtag gcaggctcca ccgactcggc cgccgcagac
     2761 cggcccaacg cgagtccaat ccccataatt gcgcccgtta acgctccgca cgtcccgcag
     2821 gtacgcgcca tcccgctgca aaagcccgtc gcgatgttcg ggatcagctc ggagcgtgtc
     2881 ccgcgggatt ccgccactgc aagcagcgcg ctctcggcgc agtagaggcc cgagagaaat
     2941 gagtccgcgg ctgtccttcc gattccggaa gctgttttct gtcctcgtgt tgtttcgtcc
     3001 atcttttcct ccttgaaaaa tgacatgatg acgggtcgat attggtcgac aataacataa
     3061 attccgtcgt ctcgcaagaa gcggaatcgc caagagaata ttcgactgca tcccgtttct
     3121 ccgcttttct tgacacgtcc ggtaaaaacg cgcacaatga acttcaagaa cgcctgtatt
     3181 cgtttcggca gtagtgtggt ggttctgtat gctgctttca gggggaatga actatgttcg
     3241 aaatgcactt tacggtcgaa acctgggggc ggagtgttct gctctcggga cgtctggatg
     3301 ccaggtcggc tccggatttt gagaaggccg cgctcgccgc agccgatgaa cagcccgaaa
     3361 agcgtcttca catgctcctc gatttttcag gactcgagta catcagcagc gcgggactcc
     3421 gttcgattct cgtgcttgcc aagcggctct ctccttcggg agggaaggtg gtgctttgcg
     3481 gaatgtccgg ggtcgtcgag gaggtcttcg gcgtttccgg ctttgacagc tttcttccgg
     3541 tgacgaagag ccgcgaggag gctcttcgcc ttctcggcga gtagaggagg aaaagacgat
     3601 ggaaaacacc gtcagagagc gggtttttcc agcctctctc gacttgttgg gagatatccg
     3661 tgccttcatt ctggaggagg acggggcgga ggagcgtctt ccgccgaagc gggtggcgca
     3721 cctggatgtt gcgatcgagg agatcgtcgt gaatatctgt tcctacgcgt acgaaacccc
     3781 tccgggcgag ctgacgatac gggtcgagga gaaacccgag tttttcgccg tggattttct
     3841 ggataacggg gtcgcgttcg accctctcgc catggacgag cctgacgttt ctcttcccct
     3901 tgaaaagcgc gacgcggggg ggctgggcat cctcctggta cggagaatga tggacgaagt
     3961 gcactactcc cggaaaggga cgttgaacag cctccgtatc gtggtggaca cgccccggat
     4021 gccgtagggg gattggacat gtcgatgttg cgcgcccttc ggcggctgat tccggccgtt
     4081 cttctcccgg tgtttgttct tctctgcgtt tgccccgccc gaagtcagga gaaggccccg
     4141 gccgcttccg gcatg
//
LOCUS       DAOTIL010000139         4149 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminobacteriaceae bacterium isolate AMR_MDS_3026
            NODE_25459_length_4149_cov_3.702491, whole genome shotgun sequence.
ACCESSION   DAOTIL010000139 DAOTIL010000000
VERSION     DAOTIL010000139.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868050
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminobacteriaceae bacterium (wastewater metagenome)
  ORGANISM  Aminobacteriaceae bacterium
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae.
REFERENCE   1  (bases 1 to 4149)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4149)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:19:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,224
            CDSs (total)                      :: 3,198
            Genes (coding)                    :: 3,184
            CDSs (with protein)               :: 3,184
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4149
                     /organism="Aminobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_25459_length_4149_cov_3.702491"
                     /isolate="AMR_MDS_3026"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:3034609"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(187..1476)
                     /locus_tag="P5201_04055"
     CDS             complement(187..1476)
                     /locus_tag="P5201_04055"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRV97737.1"
                     /translation="MRKRVELRVKDANSVPEREQLLAKILPETPKEVLEHFLIETASR
                     SSERYVELLEYISAGTSRPSVVERLAAEEKILSLWRSAEPSLSDLYEYGGLSQDDEEE
                     LYDLLDELSNVLDEGLAGRECRREITDNVFEYLEKDDYGTHELLYDIALSACTDEDDL
                     DYLSRTLEEQGDSLEERYGPASMMKYYKKSGDREKYLAIRLENLHRSEDYLDLAEFHR
                     DSGDWEEAMKVAEAGLEKADGNKYTLRSFVIDRLDATGKRARATELRFDQFFSAAFFK
                     EYLKFRRECSDEEWAAYEERVAERMKSAYPDARLFYHMHRGESSDALTILLQHGRKLY
                     WNSDVFAAAEKLEADHPERMYDCYVRSMGNLYETAGRDVYALKARLMAAVRRVLVERL
                     GEVAKWTAFAREVKKGNSRRAALQDEFAKVIPGWRKL"
     gene            1696..2175
                     /locus_tag="P5201_04060"
     CDS             1696..2175
                     /locus_tag="P5201_04060"
                     /inference="COORDINATES: protein motif:HMM:NF036995.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1016 N-terminal domain-containing protein"
                     /protein_id="HRV97738.1"
                     /translation="MEQYEQHGSNRAEYGARLLDNISSKLIGSGMEGVAARSLRQYRQ
                     FFATYPEIWQTLSAKSSEALIPSSIRQTLPLESPVSSPAREPISVERWDVFRKLSFSH
                     FAEMIAITDETKRLFYEVECIRGNWSIRELKRQIAFEEGMIARWLERLREIDETPVS"
     gene            2280..2612
                     /locus_tag="P5201_04065"
     CDS             2280..2612
                     /locus_tag="P5201_04065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRV97739.1"
                     /translation="MRGIAEVDEKIENAFMSLPSEDKSAVISHGAAIRLSELNKRAFL
                     AREKVRSFEEKYAVKLPEIEKTGLPDDAGYEMHEDYIMCSHWSDVIEKTEKQIELLRP
                     LLEYGVLR"
     gene            2613..2972
                     /locus_tag="P5201_04070"
     CDS             2613..2972
                     /locus_tag="P5201_04070"
                     /inference="COORDINATES: protein motif:HMM:NF039803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6516 family protein"
                     /protein_id="HRV97740.1"
                     /translation="MTAIDRLLEIEARFAEAVEKIVPLEFDGDALKIVLHLKDGGTLR
                     VVEQWKNARLEKYSYYWLAPDNSLRIGWDNVPHHKHVATFPHHKHVGTRDTLRPSEET
                     SLEDVLRVVLDMKSERS"
     gene            3173..3679
                     /locus_tag="P5201_04075"
     CDS             3173..3679
                     /locus_tag="P5201_04075"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRV97741.1"
                     /translation="MRLLRNGRRLRESETGKRCERWLGGVWCALGGRDYESGKRWSYR
                     EVVSAIRDIRRRRLIFSLLYASVLAGTLTFLKLQGVQSDESASRILRTLFSIAPVVFA
                     LYIKILIARSYDYLSGADAEIRGALSQPLSASKSIFDATLVFLLWNPIMDIRSLEHLG
                     EILRMLIR"
BASE COUNT          929 a         1193 c         1099 g          928 t
ORIGIN      
        1 atcctcggct tccaatctga tatttacggt cgcattcacc ataccgctca ccccccgaac
       61 gcatttgtat tgctttttgc gttacatctc tagagggatt cccgataccg gcgtccagac
      121 actctccttt ttctgatgat cgaatgagaa ggggctcgga gaaagagcac aaaaacaaga
      181 aagcaactac agcttcctcc atccgggaat gactttggcg aactcatcct gaagggcggc
      241 ccgccgggaa ttgccttttt tcacctcgcg ggcgaacgcc gtccacttcg caacctctcc
      301 caaacgctcg acaaggacgc gccggactgc cgccatcagc cgagctttga gcgcgtacac
      361 atctcttccg gcggtctcgt agaggttccc catgctccgg acgtagcagt cgtacattct
      421 ttcggggtga tcagcctcaa gcttttccgc agctgcaaaa acatcgctgt tccagtagag
      481 ctttctcccg tgctgcaaaa ggatcgtcag cgcatcggag gactctccgc gatgcatgtg
      541 atagaagagg cgagcatccg gatacgcgct tttcatgcgc tccgcgaccc tctcttcgta
      601 ggcggcccac tcttcgtcgg aacactcccg gcgaaacttc aggtactcct tgaaaaatgc
      661 cgcgctgaag aactgatcga aacgcagttc cgtcgcccgc gctctctttc ccgtcgcgtc
      721 cagcctgtct atgacgaagg agcgaagcgt gtatttattg ccgtcggcct tttcaagccc
      781 cgcctcggcc actttcatgg cctcctccca atctcccgag tctctatgga actccgcgag
      841 gtcgagatag tcctcggacc gatgtaaatt ctcaagccga atcgcgagat acttctcccg
      901 gtcgccgctt tttttgtagt acttcatcat ggaagcagga ccgtatcttt cttccagaga
      961 atccccctgc tcctcgagcg tccggctcag atagtcgaga tcgtcctcgt ccgtacacgc
     1021 cgaaagggcg atgtcgtaca gaagttcgtg ggttccgtag tcatccttct ccagatattc
     1081 gaacacattg tccgtaattt ccctccggca ttctctgccc gcaagccctt cgtcaagaac
     1141 gtttgaaagc tcatcaagca gatcgtacaa ctcctcttcg tcgtcctgac tcaaacctcc
     1201 gtactcgtaa agatccgaga ggctcggttc cgcgctccgc caaagagaga gaatcttctc
     1261 ctccgcggcg agtcgttcga ctacggatgg ccgggacgta ccggcggaga tgtattcaag
     1321 cagttcaaca tatcgctccg aagatcggga cgcggtctct atcagaaaat gctccagcac
     1381 ttctttcggc gtctccggaa gaatcttcgc aaggagctgt tcccgttccg gcacactgtt
     1441 cgcatcttta acgcgaagtt caactctttt tctcatcctt gtagatctct cctctcttga
     1501 acagtcccca aaaacagaag agatacgacc atactcttcc cccaagcgcg taataccgtt
     1561 cgcaagcggc cggcgtaccg atgatcatac tcttcccggc cagaacaata gcatgaaggg
     1621 ggaggtcgct acaagagcga tgagacgtca gtgtggtatg tttttctggc gacatgaaat
     1681 agaataggct gctatattga gcaatacgaa cagcatggat ccaaccgggc cgaatacggc
     1741 gcgagactac tggacaacat ctcgtcaaag ttaatcggaa gcggcatgga gggagtcgcc
     1801 gccagatcat tgcgtcaata caggcagttt tttgcgactt accccgagat ttggcagaca
     1861 ctgtctgcca aatcatcgga agccctgatt ccatcttcca ttcggcagac gttaccttta
     1921 gaatcacccg tttcttcgcc cgcccgggaa ccaatctcgg tcgagcgctg ggatgttttt
     1981 cggaaactct cgttcagcca ctttgcagaa atgattgcga ttacggacga aaccaagcgt
     2041 cttttctacg aagtcgagtg catccgcggc aactggtcga ttcgcgaatt gaagcgccag
     2101 atcgccttcg aggaaggaat gatcgcaagg tggcttgagc gcctgcggga gatcgacgag
     2161 acgcccgttt cgtgacgctc tttccatccg ggagacggtc ctccgataaa gtcatcgtga
     2221 tatactgtga aataatcgga tgctgtgggt atccgcgaca acgaggagga ggatgaacaa
     2281 tgcgcggtat cgccgaggtc gatgagaaga tagagaacgc ttttatgtcg ctgccgtccg
     2341 aggataaaag tgcggtgatc agccacggcg cggcgattcg cctctccgag ctgaacaagc
     2401 gcgcctttct ggcaagagag aaggtccgct ccttcgagga aaaatacgcc gtgaagctgc
     2461 cggagattga aaagacgggc cttcccgacg atgccggata cgagatgcac gaggactata
     2521 tcatgtgcag ccactggagc gatgtgatcg agaagacgga gaagcagata gagctgctgc
     2581 gtcctctgct tgagtacgga gttctacgat aaatgacggc catcgaccgg ctgctggaga
     2641 tcgaggctcg attcgccgaa gccgtagaga agatcgttcc gttggagttc gacggcgatg
     2701 cgctcaagat cgttctccac ctaaaggacg gcggcacgct gcgagtggtc gagcagtgga
     2761 agaacgcccg cctcgagaag tacagctact attggctcgc accggacaac tcgcttcgga
     2821 tcggctggga caacgtccct catcataagc acgtggcaac ctttccgcac cataagcatg
     2881 tcggcacccg ggacacgctg cggccgtccg aggaaacatc cctggaggac gtgctgcgcg
     2941 ttgtgctcga catgaaaagt gaacgttcct aggagcccgg ccgaatgaga atgcccgaag
     3001 ccgcagcggt gaaccttgca cgttttcagc ggcttggaaa aagcttgctc gatgaacagg
     3061 caaggagctg ctcccgaatg gaagagacgg cggcccattc tgccgtgccc ggtttccgat
     3121 gaaactcccc cgatggcggc ggacctacga cgagcggaag gagacatggc cgatgcgcct
     3181 tcttcgcaac ggaagacgcc ttcgcgaaag cgaaaccgga aaacgttgcg aacgatggct
     3241 cggtggtgtc tggtgcgccc tcgggggacg cgactacgag agcggcaagc gctggagcta
     3301 ccgcgaggtc gtgtccgcaa tacgggacat ccggagaaga agactgatct tctcgctgct
     3361 ctacgcctcg gttctcgccg gaacgctcac cttcctgaag cttcagggcg tgcagagcga
     3421 cgagagcgct tcgaggattc tgagaacgct cttctcgata gcgccggtcg tcttcgccct
     3481 ctacatcaag attctgatcg cgagaagcta cgactatctc tcgggcgcgg acgccgagat
     3541 acgaggagcg ctctctcagc cgctctcggc gagcaagagc atcttcgacg cgacgctggt
     3601 ctttctgctg tggaacccga tcatggacat ccgctctctg gaacatctgg gagagatcct
     3661 gcgaatgctg ataagatagc cgctcttttt ccctttctgc aacacaccgt caagcgcgtt
     3721 ctctacccct tgctgcgaga aaagcgcccg tcacagaaaa gcgcccgttt attgcacccg
     3781 tacagttttt tttcgatatt cccagagatt cggcagacgg tgtctgccaa atctcaacct
     3841 ctggttccac tcctcatcga aaatcagata cgcgaagcgg agatgacggc ggtttcggcg
     3901 cttcttccgc gttccccagc ccgcactgtt tatgcacgat tttttttgaa tttaaatgtc
     3961 cctcttggca aaaaccgaac ttgatgtact atacttatgg agatgttatc ctcgcacgaa
     4021 agctcttcat gcggatttcg cacacgataa ggagctgcca tgactggatc ggttttccaa
     4081 gcgatactcg acgcacttcg ttcggccgat gatctgtatc atcggctggt gttgcttgtc
     4141 gggcgggag
//
LOCUS       DAOTIL010000140         4147 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminobacteriaceae bacterium isolate AMR_MDS_3026
            NODE_25468_length_4147_cov_4.681574, whole genome shotgun sequence.
ACCESSION   DAOTIL010000140 DAOTIL010000000
VERSION     DAOTIL010000140.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868050
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminobacteriaceae bacterium (wastewater metagenome)
  ORGANISM  Aminobacteriaceae bacterium
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae.
REFERENCE   1  (bases 1 to 4147)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4147)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:19:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,224
            CDSs (total)                      :: 3,198
            Genes (coding)                    :: 3,184
            CDSs (with protein)               :: 3,184
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4147
                     /organism="Aminobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_25468_length_4147_cov_4.681574"
                     /isolate="AMR_MDS_3026"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:3034609"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..862
                     /gene="plsX"
                     /locus_tag="P5201_04080"
     CDS             <1..862
                     /gene="plsX"
                     /locus_tag="P5201_04080"
                     /EC_number="2.3.1.274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013048454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043811 - phosphate:acyl-[acyl carrier
                     protein] acyltransferase activity [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="phosphate acyltransferase PlsX"
                     /protein_id="HRV97742.1"
                     /translation="PLLEKAPSSLAKRLSIIHTDERIDMDEHPTLAIRKKRNSSMRLA
                     MEMVRSGEASGCVSSGNTGAIVAGGVLVVGRISGIDRPAMGVALPTLNKYTFLLDVGA
                     NVRNKPYHLFQFAHMGNIYSKRILGVTTPQIALLSNGSEDIKGDEVISGAKTLIKESA
                     LNFSGYVEGDDIPYSRADVVVCDGFSGNIVLKFAEGLMTAVASMLKEEIGHRILPKVG
                     MVFMLPMMKDLIARFHYEKHGGTPLLGVNGAVIKAHGRSKAPAIVSALRVARNFVKQN
                     GVELIREELG"
     gene            876..1871
                     /locus_tag="P5201_04085"
     CDS             876..1871
                     /locus_tag="P5201_04085"
                     /EC_number="2.3.1.180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005662325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0033818 -
                     beta-ketoacyl-acyl-carrier-protein synthase III activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase III"
                     /protein_id="HRV97743.1"
                     /translation="MSFFQGVPVAIRGSGMYAPRKVVTNNDLSQIVDTSDEWIRERTG
                     IQRRHIAEPGELTSTIAEKAGRAALENAGILPSELDMVIVATNSPDTLFPGVAPKVQG
                     LLKAENAGAFDVQSGCTSGVYSIVLAVNGIASGLWKNVLVIGAEVLSPLIDWTDRNTC
                     VLFGDGAGAMVLAPSNGNGRFISAQLKSDGTKHDQITFLGGLIENPASHETIDQKKHY
                     VAMKGNDVFKFVNRVIPPFLKTLCEDAGMLPEGIDWWFFHQANQRIIERTAERLGINP
                     ERVFINLDEYGNTSAASVFIALSEFMKQGKLLRGQKLLFTAFGAGMTYGGVIYES"
     gene            1861..2835
                     /gene="fabK"
                     /locus_tag="P5201_04090"
     CDS             1861..2835
                     /gene="fabK"
                     /locus_tag="P5201_04090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008520408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018580 - nitronate monooxygenase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-[acyl-carrier-protein] reductase FabK"
                     /protein_id="HRV97744.1"
                     /translation="MNHSTTSSHRRNRIVRLLGCSYPLIQGGMAWVADAELAAAVSNG
                     GGLGVIAAANMPPELLEQQLVKVRTLTDRPFGLNIMLMSPTVEDVIELAAKYSVPIVT
                     TGAGSPGKVIERLKPSGTIVVPVIASVAQAKRVEKQGADAVVAEGMEAGGHIGELTTM
                     VLVPQIVDSVEIPVVAAGGIADGRAVAAAFALGAEGVQVGTRFVCCTESTVHPSYKEA
                     ILAARDRSNVITGRITGHPVRCLKNKLTSRFEELEKENAPVEEYEKLGAGRLRAAVVE
                     GDVEWGSLMAGQSAALVNDILPAAEIIRNLFRDAEIISQGLSAALSLE"
     gene            2845..3792
                     /gene="fabD"
                     /locus_tag="P5201_04095"
     CDS             2845..3792
                     /gene="fabD"
                     /locus_tag="P5201_04095"
                     /EC_number="2.3.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015556184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004314 - [acyl-carrier-protein]
                     S-malonyltransferase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ACP S-malonyltransferase"
                     /protein_id="HRV97745.1"
                     /translation="MSYSLIFPGQGSQEVGMGRDIRDAFKSAAEVFQRADDALSFSLS
                     KIIFDGPEEELRRTAYTQPAILVASIAAISVLEELVGKRLQPASLAGHSLGEYTALVA
                     ARTLSLEDGVRLVHLRGRLMQEAVPEGKGAMAAILGLDAQAVSDICQEVAPLHECQPA
                     NFNSPGQIVISGETEFVEKAMLLAKEKGAKKTVLLNVSAPFHSRLMRPVADQLLTAFK
                     QCAWGNPSAPIVSNVRALPVANIQDIQDALFHQTYMPVLWEKSVHWMADSGVDTFFEI
                     GPGTVLGALVKKCRKGLTAYPCGTLATLEKTAALLKEMM"
     gene            3794..>4147
                     /locus_tag="P5201_04100"
     CDS             3794..>4147
                     /locus_tag="P5201_04100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009164754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HRV97746.1"
                     /translation="MEQRIALVTGAGKGIGRAVAIRLAKNGFFVAVNFRSSEKAALEV
                     CDIIAAGGGNAFPVKGDVSDPEEVKRVFSFVNEKAGNVSVLVNNAGITKDALLIRMKD
                     EDWNSVLTANLSSVYH"
BASE COUNT         1018 a         1016 c         1146 g          967 t
ORIGIN      
        1 gcctctgctg gaaaaagctc cttcttcgtt ggcaaagcgt ctttccatta tacacactga
       61 tgaacgtata gatatggacg aacatccaac gcttgccata cgaaaaaaaa gaaactccag
      121 catgcgtctt gcgatggaga tggttcgttc cggcgaagca tccggatgtg tatcctccgg
      181 taacacaggc gcgattgttg ccggaggagt gcttgtcgta ggcaggattt ccgggatcga
      241 ccgtcctgca atgggtgttg cgctccccac gctgaacaag tacacctttc ttctcgacgt
      301 gggcgcaaac gtacgaaata aaccgtatca tctttttcag ttcgctcaca tgggtaatat
      361 ctattcaaag cgcatcctgg gcgttacgac gccgcagata gcgctgctct caaacggctc
      421 ggaggatata aagggcgacg aggtgatctc cggcgcaaaa acgcttatta aagagagcgc
      481 gctcaatttt tcgggatacg ttgagggaga cgacatcccg tacagcaggg cggacgtggt
      541 tgtctgcgac ggcttctcgg gaaacatcgt tctcaaattc gcagagggcc tgatgactgc
      601 agtcgcttct atgctcaaag aagagatcgg ccatcggatt cttcccaagg tgggaatggt
      661 ctttatgctc cctatgatga aagatctcat tgcacgcttt cactatgaga agcacggcgg
      721 cacgccgctt ctgggcgtca acggagctgt aataaaggct catggacggt cgaaggcgcc
      781 ggctatcgtg agtgcgctga gagttgccag gaacttcgtt aagcaaaacg gcgttgagct
      841 gataagggaa gagttagggt aaaggaggtc ttctcatgtc tttttttcaa ggagtgcccg
      901 tcgccattcg cggtagcggt atgtatgccc cgagaaaagt agtgacgaat aacgacttat
      961 cacaaatagt cgatacaagc gatgaatgga ttcgtgaaag gactggcatt cagagaagac
     1021 acattgctga accgggagag ctcacgagca caatcgccga aaaggcaggg cgtgcagcac
     1081 ttgaaaatgc tggtatcttg ccttctgaac tcgatatggt cattgttgcc acgaacagcc
     1141 cggatactct attcccggga gtagccccga aagtccaggg gttgcttaaa gcagagaatg
     1201 ctggggcgtt cgacgttcag tcgggatgca ccagcggagt gtactctata gtcctcgccg
     1261 ttaacggtat agcttcaggt ctttggaaaa atgttctcgt cattggcgcc gaggtgcttt
     1321 cgccgcttat tgactggact gaccggaata cgtgcgttct tttcggcgac ggcgcgggcg
     1381 cgatggttct cgctccttcc aacggcaacg gacgctttat ctcggctcaa ctgaagtcgg
     1441 acggcacgaa acacgaccag ataacgttcc tcggagggct tatcgaaaac cctgcctcgc
     1501 acgaaaccat cgatcagaaa aaacactatg tcgcaatgaa agggaatgac gtctttaaat
     1561 ttgtcaatcg agttattccc ccgtttttga aaacgctgtg cgaagacgca ggcatgttgc
     1621 ctgagggaat tgattggtgg ttttttcatc aggcaaacca gcgaatcatt gaacgcacag
     1681 cagagcgtct cggcatcaat cccgagagag tattcatcaa tcttgacgaa tacggcaaca
     1741 catccgcggc atccgtcttt atcgctctgt ccgagtttat gaaacaaggg aaactccttc
     1801 ggggacagaa actgctcttc accgctttcg gggcaggtat gacatacgga ggtgtcatct
     1861 atgaatcata gtacaacatc gtcgcaccgt agaaaccgca tagtccgact cctcggatgc
     1921 tcctatcctc ttattcaagg aggtatggcc tgggttgcgg atgctgagct cgcagcagcg
     1981 gtaagcaatg ggggagggct aggagtcatt gccgccgcaa atatgccccc ggaactcctc
     2041 gaacaacagc ttgttaaagt gcgtacgctt accgacagac cttttgggct gaacatcatg
     2101 ctcatgtcac cgactgttga ggacgtaatc gagttagctg caaaatacag cgttccgatt
     2161 gtcaccacag gagcaggaag tcccggcaag gtcattgaac gcttgaaacc gtccggaact
     2221 atcgtcgttc ccgttattgc ttccgttgca caggctaaac gagtggagaa gcagggggcg
     2281 gacgcggtag tcgcagaagg catggaagcg ggaggacaca tcggcgaact aacgacgatg
     2341 gttctcgtac ctcaaatagt tgattctgta gagattcctg tcgttgcagc aggtggtatc
     2401 gccgacggaa gagccgtcgc ggctgcattt gcgctcggcg ccgaaggcgt acaggttgga
     2461 acacggtttg tctgttgcac agagtcaact gtccacccgt cctacaaaga ggctattctc
     2521 gcagctcggg acagaagcaa cgtcattacc ggccgtatta ctggacaccc tgtgcgctgt
     2581 ctaaaaaaca agcttaccag ccgttttgaa gagcttgaaa aagagaacgc accggtcgag
     2641 gagtatgaaa agctgggagc aggacgtctt cgcgccgccg ttgtcgaagg cgacgtcgaa
     2701 tgggggtctc tcatggctgg acagagcgcc gccctggtca acgatatcct tcctgccgca
     2761 gagattatcc ggaatctttt tcgtgatgca gaaataatca gtcaggggct ttctgcagct
     2821 ctttctttgg aatgaggtga aaagatgtca tattcgctga tcttccccgg tcagggatct
     2881 caagaagtag ggatgggcag agacattcgt gatgcattta aaagtgctgc ggaagtcttt
     2941 cagcgtgccg atgatgcact ctccttttct ctttcaaaga taatttttga tggccctgaa
     3001 gaggaactgc ggaggacggc atatacgcaa cctgccattc tcgtcgcaag catagccgca
     3061 atctccgttt tagaggaact tgtggggaaa cgtcttcaac cagcttctct cgcaggacac
     3121 agtctcgggg agtatacggc tctggtagct gcaagaacgc tctctcttga agacggcgtc
     3181 cgcctggttc atcttcgtgg gaggctcatg caggaagcgg ttcctgaggg caagggcgcg
     3241 atggcggcaa tacttggact tgatgcgcag gctgtctccg acatttgcca ggaggtcgct
     3301 cctttgcacg agtgtcagcc cgcaaacttt aattctcccg gtcagattgt catctccgga
     3361 gaaacagaat ttgttgagaa agcgatgctt ttggcaaaag aaaaaggcgc caaaaaaaca
     3421 gtgctcttga atgtcagcgc tcccttccac agccgtttga tgcgccctgt ggcagatcag
     3481 cttcttacag cttttaagca gtgcgcgtgg gggaatccct ccgcacctat tgtctccaac
     3541 gtgcgcgcgc tgcctgtagc caacatacaa gacatacagg acgccctctt ccatcaaacg
     3601 tatatgccgg tcctctggga gaaatctgtt cactggatgg cagattccgg ggtagacact
     3661 ttttttgaaa taggccccgg tacagttttg ggagcattgg tgaagaagtg ccgcaagggt
     3721 ctcacggcat atccgtgcgg aactcttgct acccttgaaa aaacagcggc tcttttaaag
     3781 gagatgatgt gatatggagc agcgcatcgc tcttgtaaca ggcgcaggca aagggatcgg
     3841 gcgggctgtg gcaatccgcc tggcgaagaa cggttttttt gtcgccgtga attttcgcag
     3901 cagcgagaag gcagccttgg aagtttgtga tatcattgcc gccggaggag gaaacgcctt
     3961 ccccgtcaaa ggagatgtgt ccgatcccga agaggtaaaa cgggtattct cttttgtcaa
     4021 tgaaaaagcc gggaacgtaa gcgtactcgt gaacaatgcc ggaattacaa aggacgccct
     4081 gctcatcaga atgaaagacg aagattggaa ttcggtgctg acagcgaatc tctcctcggt
     4141 gtatcat
//
LOCUS       DAOTIL010000141         4140 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminobacteriaceae bacterium isolate AMR_MDS_3026
            NODE_25529_length_4140_cov_3.480049, whole genome shotgun sequence.
ACCESSION   DAOTIL010000141 DAOTIL010000000
VERSION     DAOTIL010000141.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868050
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminobacteriaceae bacterium (wastewater metagenome)
  ORGANISM  Aminobacteriaceae bacterium
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae.
REFERENCE   1  (bases 1 to 4140)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4140)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:19:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,224
            CDSs (total)                      :: 3,198
            Genes (coding)                    :: 3,184
            CDSs (with protein)               :: 3,184
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4140
                     /organism="Aminobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_25529_length_4140_cov_3.480049"
                     /isolate="AMR_MDS_3026"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:3034609"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..352
                     /locus_tag="P5201_04105"
     CDS             <1..352
                     /locus_tag="P5201_04105"
                     /inference="COORDINATES: protein motif:HMM:NF019448.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRV97747.1"
                     /translation="TPEKILPLAGKKHTITASEDSTVRALNARKIGEGVKRLGGGRMS
                     LGEPIDLSVGVRILKKNGASAGRGEALLEILYSDEAKLADAMPFFESAYTFGKANEHS
                     EDTAQRRLILGKIG"
     gene            336..1280
                     /locus_tag="P5201_04110"
     CDS             336..1280
                     /locus_tag="P5201_04110"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: protein motif:HMM:NF013700.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M23 family metallopeptidase"
                     /protein_id="HRV97748.1"
                     /translation="MGRSGRFTFFRFGSILFLLFCLVCIPLSASAEVTVQYPSSVGVG
                     KPFLVRVTSSVPLTGVEAVWQGRTVQLGVSVWNERYISLALLGTQVGKVKTGDHRLTL
                     RLLSGGKRSEHPFSIKVMPVQYKEDRLTLPEKMVTPPSSELKRIEAERKRTGAALATM
                     TAGRQWGLPIIRPVSGIVTSPYGRRRILNGKPRSPHAGVDFRAAVGTPVLAALPGKVV
                     LTGDHYYAGKSIYIDSGGGVISHYFHMHTIDVKKGDPVKPGQVIGKSGVTGRATGPHL
                     HFGLALAGQMVNPEPLFSATSSGMLEKTALRKVTPRWK"
     gene            1335..2030
                     /locus_tag="P5201_04115"
     CDS             1335..2030
                     /locus_tag="P5201_04115"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRV97749.1"
                     /translation="MRGWKRAFGSCALLLFFASAGWASSLIADYEARFTKYFTDRIQA
                     QSVLSVIKASEDELRRGKIPTLNLCIEDAVVEGVRYDRLLLVMHDVLFTSAARGIVVH
                     SHGESHLSGGISKDTFLKTLSEGMPHFAVSELALRNGSVMIKGVYERRLTFKVRALMR
                     FTGSYVIDGDGVARVRFDDASNDNPAISTLDVAKAMANAASVLSFRDFFGAPLVREVL
                     VDHDMVWFSARNQ"
     gene            2101..2198
                     /locus_tag="P5201_04120"
     tRNA            2101..2198
                     /locus_tag="P5201_04120"
                     /product="tRNA-Sec"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2135..2137,aa:Sec,seq:tca)
     gene            2291..2941
                     /locus_tag="P5201_04125"
     CDS             2291..2941
                     /locus_tag="P5201_04125"
                     /EC_number="2.1.1.77"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014806792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004719 - protein-L-isoaspartate
                     (D-aspartate) O-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein-L-isoaspartate(D-aspartate)
                     O-methyltransferase"
                     /protein_id="HRV97750.1"
                     /translation="MIPWEKRAEEMVEKQIRARGILSERVLEAMRSVPRHLFLPEHLR
                     ESAWIDSPLPIGEEQTMSQPYMAAKMTELLAPEEGDSVLEIGTGSGYQSAVLCSMGAK
                     VTSIERIAPLAERARETLSSLGYDVSVLWSDGREKGELAGEFQGVIVTAAAPALEAWW
                     SEALAPGGRLVAPVTVLSGSERLLLREKKAPGEYVDTWHDYCRFVPLLQGRTGGYC"
     gene            2954..3469
                     /locus_tag="P5201_04130"
     CDS             2954..3469
                     /locus_tag="P5201_04130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013048391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="QueT transporter family protein"
                     /protein_id="HRV97751.1"
                     /translation="MKVSFFKGASPKELFSVRSLALSGLVAALYAVTTILFAPISYGY
                     VQVRISEALTVLPFLFPQAVFGLFVGCLFANLFGGFGILDVVLGSAATLLAGIASSAM
                     PTVYLAALPPVLVNMVFVGGYLSFILDLPFPLCAMYVGIGQFIACYLLGVPLTLLLGK
                     RIKAGGEDPAR"
     gene            complement(3474..>4140)
                     /locus_tag="P5201_04135"
     CDS             complement(3474..>4140)
                     /locus_tag="P5201_04135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009165798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="class I tRNA ligase family protein"
                     /protein_id="HRV97752.1"
                     /translation="VRYWIHNGYLLIDKEKMSKSLGNFLTARAALQKYPAKAIRLFML
                     SAHYRSPINFSEESLNQSLGAVERLENCWSDLEHARKNRKTTSDGSRNEFESLFKEFE
                     DRFITAMDDDFNTAAALGVLFEAVKTVNTYLKETQTLEESFLELAAEFFRKIDSVLGV
                     LGLDAAAGEEDAEEIEALIAERNAARKNKDFKRSDAIRDDLAARGILLEDTPDGTKWK
                     KKA"
BASE COUNT          799 a         1052 c         1290 g          999 t
ORIGIN      
        1 cactcccgag aaaatccttc cgcttgccgg caaaaaacat acaatcactg catctgaaga
       61 ctccacggtg cgcgccctga acgcccgcaa gatcggcgaa ggcgtcaagc gcctcggtgg
      121 agggcgcatg tcgctgggag aacctatcga cctttcggtg ggagtgcgga ttctgaagaa
      181 gaacggcgcc tccgccgggc ggggggaagc gctgcttgag attttgtact ccgacgaggc
      241 gaaactggca gacgccatgc cgttttttga atccgcctat acgttcggca aagcgaacga
      301 acatagcgaa gatacagcgc aaaggagact tattcttggg aagatcgggt agatttacgt
      361 ttttccgttt cggcagcatt ctgtttctgt tgttctgcct cgtttgtatc ccgctgtccg
      421 cttcggccga ggtgactgtt cagtacccct cctcggtggg cgtcgggaag ccttttttag
      481 tgcgcgtcac ttcctccgtc ccgttgacgg gagtggaggc cgtttggcag ggacgtacgg
      541 tgcaactcgg agtttccgtg tggaacgaac ggtacatttc gctcgcgctt ctcggaacgc
      601 aggtcggcaa agtgaagacc ggagatcatc gtctgacgct ccgtctgctc tcagggggga
      661 agcgttctga acatcctttc tcgatcaagg taatgccggt tcagtacaag gaggaccgcc
      721 tgacgctgcc tgagaagatg gtgactccgc cttcttcgga actcaagcgg atagaagccg
      781 aaagaaagcg tacgggcgcg gcgcttgcca caatgacggc aggtaggcaa tggggtctgc
      841 cgattatcag gccggtatcc ggtattgtaa caagcccgta cggaagacgg cgcatactga
      901 acggaaaacc gaggagtccg catgcggggg ttgacttcag ggcggcagtc ggtactccgg
      961 ttctcgcggc gcttcccgga aaggtcgtgc tcaccggaga tcattactac gccgggaaga
     1021 gtatctatat cgattcgggc ggcggggtta tatctcacta ctttcatatg cacacgatcg
     1081 acgtaaaaaa aggagatccc gtcaagccgg ggcaggtgat cgggaagagc ggcgtaacgg
     1141 gcagggcaac cgggccgcat ctgcatttcg ggcttgcgtt ggcgggccag atggtgaatc
     1201 ctgaacccct tttttcggca acgtcttccg ggatgctcga aaaaacggcg ctccggaaag
     1261 taacccctcg ctggaagtga tgcaggggga tgaggtcgga tttctgtatg ctttcgccgg
     1321 gaggagggaa gcggatgaga ggttggaaga gagccttcgg cagctgcgcg cttttgcttt
     1381 ttttcgcgtc ggctgggtgg gcgagttctc ttatagcgga ctatgaggcg cgttttacga
     1441 agtatttcac ggacaggatc caggcgcaat cagtattgtc cgttattaag gcctcagagg
     1501 atgagcttcg gcggggcaag attccgacgt tgaacctgtg catcgaggat gccgtcgtcg
     1561 agggcgttcg ctacgacagg ttgctgctcg tgatgcatga tgttctcttt acttcggcgg
     1621 cacgcggcat tgttgtgcat tcgcacggtg agagccatct ttctggcggc atatccaagg
     1681 atacatttct gaaaacgctc tccgagggaa tgccccactt tgccgtatcg gaactggcgc
     1741 ttcgcaacgg gagcgtcatg atcaaaggcg tgtatgaacg gcggcttacg ttcaaggtcc
     1801 gggcgttgat gcgctttaca ggatcttatg tgatcgacgg agacggcgta gcaagggtgc
     1861 gcttcgacga cgcctcgaac gacaatccgg caatcagcac gctcgatgtg gcaaaggcga
     1921 tggcgaatgc agcgtccgtg ctctccttcc gggatttttt cggagctccc ctcgtcaggg
     1981 aggttttggt ggatcacgat atggtgtggt tttcagcaag gaatcaatag aaagattgtt
     2041 gcgctgctcc gggatgctgg actttttcac cggaagccag tacaattctt gacggcgaat
     2101 ggaggcgaat gtgtcccggt gggcgccccg gacttcaaat ccggtgggag gcggtatagg
     2161 ccgtctccgg tgggttcgac ccccacacgc ctccgccagt agtgtaaacc aaagagacgc
     2221 ttcccttcaa ggggaggcgt cttttgcatg gtacaataat ccgaaaacac accatttcgg
     2281 aggtgatttc gtgattccat gggagaaaag agcggaagag atggtggaaa aacagatccg
     2341 cgcgaggggc attctttcgg agagagttct tgaggcgatg cgctccgtcc cgcgtcatct
     2401 tttccttccg gaacaccttc gtgaaagcgc gtggattgac agccctctcc cgataggcga
     2461 agaacagacg atgtcgcagc cgtacatggc ggcaaaaatg acggagcttc ttgcaccgga
     2521 agaaggagat tcggttcttg agataggaac ggggtccggg tatcagtccg ccgtcctttg
     2581 ctcgatgggc gcgaaggtca caagcattga acgaatcgcc ccgctcgctg aaagggcgag
     2641 agagacgctt tcttccctgg ggtacgatgt ttccgtgctg tggtcggatg ggcgggagaa
     2701 gggggagctt gcaggcgagt tccagggtgt cattgttaca gccgccgcgc ctgcattgga
     2761 ggcgtggtgg tccgaggcgc tggcgcctgg aggaaggctg gttgcgccgg taacggttct
     2821 ttccggaagc gaacggttgc ttctccgtga aaagaaggcg cccggcgaat atgtggatac
     2881 gtggcacgat tactgccgtt ttgtacctct tctgcaaggg aggacgggcg gatattgctg
     2941 aagaggtgat agggtgaaag tcagtttttt taaaggagcg tcgccgaagg agttgttctc
     3001 ggttcgttct ctggcgcttt cggggcttgt cgcagctcta tacgcggtga cgacgatttt
     3061 gttcgccccg atttcctacg gatatgttca agtgagaatc tccgaagcgc ttactgtgct
     3121 tccgtttctt ttcccgcagg ctgttttcgg ccttttcgtg gggtgccttt ttgcgaatct
     3181 ctttggcggt ttcggcatac tcgatgtggt tctggggagc gccgcgacgc tgttggcggg
     3241 gatcgcctct tctgccatgc cgaccgtcta tttagccgcg ctcccgcctg tgcttgtgaa
     3301 catggttttc gtgggggggt atctatcgtt tattttggac ctcccctttc ccctctgcgc
     3361 gatgtatgtc gggatcgggc agtttattgc ctgctatctc ctcggtgttc cgctgacgtt
     3421 gctgcttggg aagcgcataa aagcgggcgg ggaggacccc gcccgctgag tcgtcacgct
     3481 tttttcttcc acttcgtgcc gtcgggggta tcctccaaaa ggatgcctct tgcggccaga
     3541 tcgtcacgga ttgcgtccga tcttttgaag tctttgttct ttcgtgcggc gttgcgttcc
     3601 gcgataagcg cctcgatctc ctcggcgtct tcctcgccgg ctgctgcgtc gagcccgaga
     3661 acgcccagaa cggagtcgat cttccggaag aattccgcgg ccagttcaag gaatgattct
     3721 tcgagcgtct gcgtttcttt cagataggta ttgaccgtct ttacggcctc gaagaggacg
     3781 ccgagggctg ccgcggtgtt gaagtcgtcg tccatagccg ttataaaacg gtcctcgaat
     3841 tctttgaaga gtgattcaaa ctcgttccgg gatccgtcgc ttgtcgtttt tctgtttttc
     3901 cgtgcgtgct caaggtcgga ccagcagttc tccagacgtt caaccgcgcc gagcgactgg
     3961 ttcaggctct cctccgaaaa gttgatgggc gacctgtagt gagcgctcag catgaagagg
     4021 cggattgcct tcgcggggta cttctgaagc gcggctctgg cagtaagaaa gttgccgagc
     4081 gactttgaca tcttctcctt gtcgatcagg agatagccgt tatggatcca gtagcgcacg
//
LOCUS       DAOTIL010000142         4125 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminobacteriaceae bacterium isolate AMR_MDS_3026
            NODE_25653_length_4125_cov_3.033907, whole genome shotgun sequence.
ACCESSION   DAOTIL010000142 DAOTIL010000000
VERSION     DAOTIL010000142.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868050
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminobacteriaceae bacterium (wastewater metagenome)
  ORGANISM  Aminobacteriaceae bacterium
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae.
REFERENCE   1  (bases 1 to 4125)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4125)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:19:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,224
            CDSs (total)                      :: 3,198
            Genes (coding)                    :: 3,184
            CDSs (with protein)               :: 3,184
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4125
                     /organism="Aminobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_25653_length_4125_cov_3.033907"
                     /isolate="AMR_MDS_3026"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:3034609"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            57..521
                     /locus_tag="P5201_04140"
     CDS             57..521
                     /locus_tag="P5201_04140"
                     /inference="COORDINATES: protein motif:HMM:NF013731.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheW"
                     /protein_id="HRV97753.1"
                     /translation="MAEQQLVVFALGKEEFGIDISRVREIVRLQNITTIPQSMDFVEG
                     IVNLRGQIVPIVDLCKRFLVADRSGSSDTERRIIVVNMSGQNIGILVDGVSEILRIPD
                     ESIEPTPPIVASGVSSDFIRGVAKVDGRLIIFLDLDRIFSVEEQEALYQSVE"
     gene            727..1686
                     /locus_tag="P5201_04145"
     CDS             727..1686
                     /locus_tag="P5201_04145"
                     /EC_number="4.1.2.14"
                     /EC_number="4.1.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015556400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016830 - carbon-carbon lyase activity
                     [Evidence IEA];
                     GO_function: GO:0016832 - aldehyde-lyase activity
                     [Evidence IEA];
                     GO_function: GO:0016833 - oxo-acid-lyase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 4-hydroxy-2-oxoglutarate
                     aldolase/2-dehydro-3-deoxy-phosphogluconate aldolase"
                     /protein_id="HRV97754.1"
                     /translation="MNDILQRIGELGIVPVVKLDKASDALPLGKALLEGELPIAEVTF
                     RTDAAEESIRIMSRELPELLVGAGTVLTRDQVKKAVDAGAKFIVTPGFNPSVVDYCVE
                     HNIPITPGINSPSQIEMALERGLDVVKFFPAEQSGGLPMLKAMSAPYGSVRFIPTGGV
                     DAKNMPSYLAFNKILAVGGSWMVKPEVVAEGKFDEIARMTREAVFAMHGFELIHLGIN
                     CPDEKEALAGAERLAALFGMAVKDGNSSAFVGKGFEFMKSMYLGEKGHVAVGCNDVMR
                     AAAFLKRKGIASLPETEKKKDGRTVALYLDLHIGGFIFHLLQK"
     gene            complement(1856..2887)
                     /locus_tag="P5201_04150"
     CDS             complement(1856..2887)
                     /locus_tag="P5201_04150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015556405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar kinase"
                     /protein_id="HRV97755.1"
                     /translation="MSRFVTFGEIMLRLTPPEHEVLFQTDRLVATFGGAEANVAVSLA
                     NYGENVSYVTAAPANPVGDAMIKEVRSFGICTDHILRKGERLGIYFTETGAAMRPSKV
                     VYDRAGSSIAAVQPGDFDWDKILDGATWFHTTGITPSLAEGTAAVALEAVKACRAKGI
                     TVSCDLNYRKKLWKWGKSAKDVMTEFAKYVDVTIANEEDCQKCLGIELDVDVTSGALD
                     TAKYKVLAEKVMAAYPNMKYLAVSLRESVSADWNGWSAVLTDSKGNFYTSKKYEIRDI
                     VDRIGGGDSFGSGLIWGLNNLKTPQDALEFSVAASALKHTVYGDFNRVSKEDVLTLMG
                     GDASGRVQR"
     gene            complement(3015..>4125)
                     /gene="fdhD"
                     /locus_tag="P5201_04155"
     CDS             complement(3015..>4125)
                     /gene="fdhD"
                     /locus_tag="P5201_04155"
                     /inference="COORDINATES: protein motif:HMM:TIGR00129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009326 - formate dehydrogenase complex
                     [Evidence IEA];
                     GO_function: GO:0008863 - formate dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_process: GO:0009061 - anaerobic respiration [Evidence
                     IEA];
                     GO_process: GO:0015942 - formate metabolic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="formate dehydrogenase accessory
                     sulfurtransferase FdhD"
                     /protein_id="HRV97756.1"
                     /translation="DGDGHFGFAHRQVSLLESEEIEKMKKVIPTLFHGAFAENLVTEG
                     IDLKRLILGDTLRIGDALLRVTQIGKECHSHCAIYEAAGDCIMPRLGIFCEVLSEGTV
                     TVGDTIEFVHEDAPQVLVPRTITRLDGTKRETVTDRLLKEGRVTLVLDGEEEVTAVCT
                     PGEERYWAVGHLKCRRLLKSREDIASLEITPGAVSVKRKQMTEGLPLLQRILSSSASA
                     LLAEGAEEQLGGVIPSEWRIPASVLREGAEWLSEAPIFRATGGTHVAALMARDGEKLF
                     RTEDIGRHNAVDKAVGWAVLHDVPLGESALLVSGRLPVDMVMKALGAGIPVLGSVSAA
                     IAEGAEAAEKGGLTLAGFIRGERMNIYTHPERISD"
BASE COUNT          741 a         1318 c         1271 g          795 t
ORIGIN      
        1 gttagaattg tcctgaagag tttattcagc aggatcttgc ggaaggagga aaaaaaatgg
       61 cggaacaaca gcttgtcgtg ttcgctctcg gcaaggagga attcggcatc gacatctccc
      121 gagtccggga gatcgtcaga ctgcagaaca tcaccaccat cccccagtcc atggacttcg
      181 tcgaggggat agtcaatctt cgggggcaga tcgtcccgat cgtcgatctg tgcaagaggt
      241 ttctggtcgc ggacagaagc ggctccagcg atacggaacg ccggatcatc gtcgtcaaca
      301 tgtcgggaca gaacatcggc atcctcgtgg acggagtctc cgagatactt cgcatcccgg
      361 acgagtcgat cgagccgacg cctcccatcg tggccagcgg cgtcagctcg gacttcatcc
      421 ggggtgtggc caaggtcgat ggcaggctga tcatcttcct tgatctcgac cgcatcttct
      481 cggtcgagga gcaggaagcg ctctaccagt cggttgagta gcgcgaaccc aaatgcatgg
      541 cggaccccgt agcgatacgg ggtctttttt attcgctttt tttgggaacg tctccctacc
      601 ggccgaaaat ctcgccgcga atttcgaaac aatgttttat tctgcggaaa aaagtccttg
      661 acgaaagggc gattcattgt ctatgcttga cgcgtcagcc acataggaaa ggagacgaaa
      721 aataatatga acgacatact gcagcgcata ggggaactcg gcatcgttcc cgtggtcaaa
      781 ctcgacaagg cctccgacgc tctccctctc ggcaaggcgc ttcttgaagg ggaactcccc
      841 atcgcggagg tgaccttccg taccgacgcg gcggaggagt ccatccgcat catgtcgcgc
      901 gagcttccgg agctgctggt gggcgccggg accgtgctca cccgcgacca ggtgaagaag
      961 gccgtggacg cgggggcgaa gttcatcgtt acgccggggt tcaacccctc ggtcgtggac
     1021 tactgcgtcg agcacaacat ccccataacc cccggcatca acagcccgtc gcagatcgag
     1081 atggcgctgg agcgcgggct cgacgtggtg aagttcttcc cggccgagca gtcgggcggt
     1141 ctgcccatgc tcaaggcgat gagcgctccg tacggaagcg tccgcttcat ccccaccggc
     1201 ggcgtcgacg cgaagaacat gccctcctac ctcgccttca acaagatcct cgccgtgggc
     1261 gggagctgga tggtgaagcc cgaggtcgtc gcggagggga agttcgacga gatcgcccgc
     1321 atgaccagag aagccgtctt cgcgatgcac ggcttcgagc tgatccacct gggaatcaac
     1381 tgtcccgatg aaaaagaggc gcttgccggc gccgagaggc tcgccgccct cttcggcatg
     1441 gcggtgaagg acgggaacag ctccgccttc gtcgggaagg gcttcgagtt catgaagagc
     1501 atgtacctcg gcgagaaggg gcacgtcgcc gtcggctgca acgacgtcat gcgcgccgcc
     1561 gccttcctga agcggaaggg gatcgcctcc ctcccggaga cggagaagaa gaaggatggc
     1621 cgcaccgtcg cgctctacct cgacctccac atcggcggct tcatcttcca cctgctgcag
     1681 aagtagcctc ccgtcccgaa agaacagggg cctccggagt attcggaggc ccctgtcgct
     1741 ttgaagagaa aaccgcacgc ggaaatcggc gcgcaccgat cggcgttgta ggcggctgtc
     1801 cctgaggctg catttccccc acagcggctt tttcgtcgtc cgagcgctcg agcttctacc
     1861 gctgtacgcg tcccgaggcg tcgccgccca tcagggtgag gacgtcctcc ttggagacgc
     1921 ggttgaagtc gccgtagacg gtgtgcttca gcgcggaggc cgccacggag aattcgaggg
     1981 cgtcctgcgg cgtcttcagg ttgttcagcc cccagatgag gccggagccg aaggagtcgc
     2041 cgccgccgat gcggtcgacg atgtcgcgga tctcgtactt cttgctggtg tagaagttcc
     2101 ccttgctgtc ggtaagcact gcggaccagc cgttccagtc ggcgctcacg ctctcgcgga
     2161 ggctgacggc gaggtacttc atattcgggt aggcggccat caccttctcc gcgagcacct
     2221 tgtacttcgc ggtgtccagc gcgccggacg tcacgtccac gtcgagctcg atcccgagac
     2281 acttctggca gtcctcctcg ttcgcgatgg tcacgtcgac gtacttggcg aactcggtca
     2341 tcacatcttt ggcgctcttc ccccacttcc agagtttctt ccggtagttc aggtcgcacg
     2401 acacggtgat tcccttcgcg cggcaggcct tcacggcctc cagcgccacg gcggccgtcc
     2461 cttcggcgag cgagggggtg atgccggtgg tgtggaacca ggttgcgccg tcgagaatct
     2521 tgtcccagtc gaagtcgccg ggctgcacgg cggcgatgga cgagcccgcg cggtcgtaga
     2581 ccaccttcga ggggcgcatg gccgcgcccg tctcggtgaa gtagatgccc agccgctccc
     2641 ccttgcggag gatgtggtcg gtgcagatgc cgaacgagcg gacttccttg atcatcgcgt
     2701 cgcccaccgg gttggcgggg gcggcggtga cgtacgagac gttctctccg tagttggcga
     2761 gcgagacggc gacgttcgcc tccgcgccgc cgaaggtcgc gaccagacgg tccgtctgga
     2821 agagcacctc gtgttcgggc ggcgtgaggc gcagcataat ctctccgaac gtgacgaatc
     2881 gggacattct ctctacctcc ttaacgacat ataatttcac ttgttgaaac gtcggttctt
     2941 tagatgaacc acaactacac tatcgccttt tcggcaatct gtcaacaaaa aacggccccg
     3001 tgaagggccg tttttcagtc cgaaattcgt tccgggtgcg tgtagatgtt catccgctcc
     3061 ccccgtatga agcccgcgag ggtgagtccg cccttctccg ccgcctccgc gccctcggcg
     3121 atggccgccg agacgctccc cagcacgggg atgcccgcgc cgagcgcctt cattaccatg
     3181 tcgacgggga gcctgccgga gacgagcaac gcgctctccc cgagcgggac gtcgtggagc
     3241 accgcccatc cgaccgcctt gtccacggcg ttgtgccgtc cgatgtcctc ggtgcggaag
     3301 agcttctccc cgtccctcgc catcagcgcg gcaacgtgcg tgccgccggt tgcgcggaag
     3361 atgggggctt cgctcagcca ctccgcgccc tcgcggagca ccgaggcggg gatgcgccac
     3421 tccgagggga tgactccccc gagctgttcc tccgccccct cggccaacag cgccgacgcc
     3481 gacgaggaga ggattcgctg caagagcggc agcccctccg tcatctgctt gcgcttcacc
     3541 gagaccgcgc cgggggttat ttcgagactc gcaatatctt cgcgactttt caaaaggcgg
     3601 cggcacttca agtggccgac ggcccagtag cgctcctcgc caggcgtaca gacggccgta
     3661 acctcttctt cgccgtcgag aacgagagtg acccgtcctt ccttcagcag tctgtcggtg
     3721 acggtctccc tcttcgttcc gtcgagccgc gtgatcgtcc gcgggacgag cacctgcgga
     3781 gcgtcctcat ggacgaactc gatcgtgtcg ccgaccgtta ccgtcccctc cgagagcacc
     3841 tcgcagaaga tcccgaggcg cggcatgatg cagtcgcccg cagcctcgta gatggcgcag
     3901 tgcgagtggc actccttgcc gatctgcgtc acccgcaaca gcgcgtcccc gatgcggagc
     3961 gtgtcgccga ggatgagtct tttgagatcg atcccctcgg tgaccaggtt ttcggcgaac
     4021 gctccgtgga agagcgtggg gatgactttc ttcattttct cgatctcttc cgactccagc
     4081 aggctcacct gccggtgggc gaagccgaag tgtccgtcgc cgtcg
//
LOCUS       DAOTIL010000143         4114 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminobacteriaceae bacterium isolate AMR_MDS_3026
            NODE_25734_length_4114_cov_3.917714, whole genome shotgun sequence.
ACCESSION   DAOTIL010000143 DAOTIL010000000
VERSION     DAOTIL010000143.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868050
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminobacteriaceae bacterium (wastewater metagenome)
  ORGANISM  Aminobacteriaceae bacterium
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae.
REFERENCE   1  (bases 1 to 4114)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4114)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:19:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,224
            CDSs (total)                      :: 3,198
            Genes (coding)                    :: 3,184
            CDSs (with protein)               :: 3,184
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4114
                     /organism="Aminobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_25734_length_4114_cov_3.917714"
                     /isolate="AMR_MDS_3026"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:3034609"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(81..1070)
                     /locus_tag="P5201_04160"
     CDS             complement(81..1070)
                     /locus_tag="P5201_04160"
                     /inference="COORDINATES: protein motif:HMM:NF015445.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter substrate-binding protein"
                     /protein_id="HRV97757.1"
                     /translation="MKRGILVVCALAFVFLFALPAAYAAVELKLGHFGPEGHPATIAA
                     AQFAENVEKRTNGEVKIVVYPNNALGSPPEVLEQVIMGVIDMSLSGQDQIAKHVKLFD
                     SVAIPFSMQGYEHADRILDGPFKEWAAPELEKIGLVYINSWEWGFRQITNSKRPILSP
                     DDVKGLKIRTPPAMAYQAAIEALGGNVLTIAFGELVMAMRQGVVDGQENPIGVIYDLK
                     LYESQKFISIVNYLYSSMTHVVSKKVFDSLTEEQRNIIIEESNKARLLMRELVRKQEQ
                     EQIADMRAKGIQIDTPDLTSFQAAMGPAYEKIKKNIGEENFNKWMEMAKATAE"
     gene            complement(1330..>4114)
                     /locus_tag="P5201_04165"
     CDS             complement(1330..>4114)
                     /locus_tag="P5201_04165"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HRV97758.1"
                     /translation="EGNMLAINETWRRFARENQAKDFRTLDTGANYFAACACSSDTED
                     YRDAQRAMAGIRSVLSGRSESFEMEYPCHSPDEKRWFLMRVTPLLFPEGGAVVAHIDV
                     TKEKVATQTVKESEQRLLAVLNSIDALVFVTDVDTFEILMINDFGRKIFGDVVGRRCW
                     EAIQKGQTGPCEFCAEAPLLGDDGEPAGVRRWQVFNERTKRWYDCRDSAIRWTDRKYV
                     RLEIAMDITETKKIEEELRVHRDELDRLVAVRTEELSESNRELERQTELAQEMVLKAE
                     AANEAKSAFLANVSHEIRTPINAVIGFTELLLDSTLSSEQRRQAETVRASAETLLFLI
                     NEILDFAKIEAGKLELEVVDFDLRNTVEGFAGTLALQAYTKGIELACGVEPSLPSKVR
                     GDPVRIRQILMNLGSNAVKFTQEGETVISVSKESETEETLWIRFSVRDTGIGIPGEKM
                     GLLFTKFTQLDASTTRQYGGTGLGLAISKHLVERMGGSIGVESEHGNGSTFWFVLPFE
                     KNGRGAEAPEAPEGVAGLRILVVDGNAASQAFFLRQFESWGMRAVVSSRTEALDVLRR
                     GFSDEDPFRVVLDADSGRSGEHSLGDAIMNDESLSETVLITLVPLGIRHGINPPDGER
                     RYSLVKPVQSAELAAALTAIALGGTVFSVAHEDGKESSSWLLSCGENARILLVEDNAV
                     NRQVALALLKKAGVRADTAENGLEALEALRSNDYDLVLMDLQMPVMDGLAATLAIRAG
                     RGDVRNPDVPVVAMTAHALQSDREACMHAGMNGYITKPIRPAELLCELERRLCAETAP
                     ARDNRREYVTRTGPRTFNFDDLVERLGDEETAGLIMEAFLKEMPEAMSALEKSLRTGD
                     FLLSGRNAHTIKGVAGNIGGEALREAALQFERAAKDGDTKLLHALRERVHAEYCALKD
                     EIERMLRTLRSPE"
BASE COUNT          822 a         1278 c         1090 g          924 t
ORIGIN      
        1 gtttcactcg gtgcggagaa gagaacggaa gggccaagcg cccgaaaacg gacgcccggc
       61 ccggcgattg caaagactgg ctattcggct gtggcctttg ccatctccat ccacttgttg
      121 aagttctcct caccgatgtt cttcttgatc ttttcgtacg ccggccccat tgcggcctgg
      181 aaggaagtaa gatccggggt atctatctga atccccttcg cgcgcatatc ggcgatctgc
      241 tcctgctcct gtttgcggac aagctcgcgc atcagcagac gggccttgtt cgactcctcg
      301 atgatgatgt tgcgctgctc ttctgtcagc gaatcgaaga cctttttcga aacgacgtgc
      361 gtcattgagc tgtagaggta gttgacgata gagataaact tctgggattc gtagagttta
      421 agatcgtaga tgacgccgat ggggttctcc tgcccgtcga cgaccccctg ccgcatcgcc
      481 atgaccaatt ccccgaaagc gatggtgagg acattaccgc cgagcgcctc gattgccgcc
      541 tgatacgcca tcgcgggcgg ggtgcggatt ttcaggccct tcacatcgtc cggggagagg
      601 atgggacgtt ttgagttggt gatctgccgg aagccccact cccagctgtt gatatagacg
      661 aggccgatct tctcgagctc gggggccgcc cactccttga agggaccgtc gagaatgcgg
      721 tccgcgtgct cgtacccctg catcgagaag gggatggcca cggagtcgaa cagcttcacg
      781 tgctttgcga tctgatcctg tcctgagagg ctcatgtcga taacgcccat gatgacctgt
      841 tccaggactt cggggggact cccgagagca ttgttcgggt acacgacgat cttgacttcg
      901 ccattcgtcc gtttttcaac attctccgca aactgcgcgg cggcaatggt ggccggatgt
      961 ccttccggtc cgaagtgacc cagtttcagc tcgaccgccg cgtatgcggc agggagcgcg
     1021 aacagaaaca cgaacgcgag cgcgcacact accagaattc ctcgtttcac aaaaacccct
     1081 cctttatgat ggtgttgcct tgaatccgcc agggaaaccc gtaggcgggc gtagccagag
     1141 gagaaacggc acagagtgtt tcaacaagca aaacactatt ccatcaaatg ctcgttcata
     1201 ttttcagtat tgagtgtgcg tttgtcaagt ctttcttgct ctccctttaa aaataaaaat
     1261 ggagcgctcc ggagaaactc catgaacgct ccattttccg tatgatcttg ctaagggtac
     1321 cccgcgggct cactccgggg agcgaagcgt tcgcagcatt ctttcgattt catccttcag
     1381 cgcgcaatac tccgcatgca cccgctcccg aagagcatga agaagcttcg tatcaccgtc
     1441 tttggccgct cgctcaaact gaagggccgc ttcccgaagc gcctctcctc cgatgtttcc
     1501 cgccacgcct ttgatcgtat gggcattcct ccccgagagg agaaaatccc ccgtccggag
     1561 actcttctcc agggcgctca tggcttccgg catctctttg aggaaggctt ccataatgag
     1621 gccggccgtc tcctcgtcgc caaggcgttc cacaagatcg tcgaaattga atgtgcgcgg
     1681 tcccgtccgc gtaacgtatt ctctccgatt gtctcgcgcc ggggccgttt ccgcgcagag
     1741 cctgcgctca agctcgcaaa gaagctccgc cggcctgatg ggtttggtga tgtagccgtt
     1801 cattcctgcg tgcatgcacg cttctctgtc actctggagc gcatgagccg tcatcgcaac
     1861 caccggcacg tccggattgc gcacatcgcc ccgcccggcc cgtatcgcca gggtcgccgc
     1921 cagaccgtcc ataacaggca tctgaaggtc cataagcacc agatcataat cgttcgaacg
     1981 cagagcttcc aaggcttcca gaccgttttc ggccgtatcg gcgcgcacac ccgccttctt
     2041 cagtagagca agcgccacct gccggtttac tgcattgtcc tcaacgagca gtatacgcgc
     2101 gttctccccg catgaaagaa gccatgagga agattccttc ccatcctcgt gtgcaacaga
     2161 aaacaccgtg cctccgaggg caatcgcggt gagcgccgca gcaagctcgg cgctctggac
     2221 cggcttcacc aatgaatagc gcctctctcc gtcgggagga ttgataccgt gccgtattcc
     2281 gagagggaca agcgtgatga gaacagtttc cgagaggctc tcgtcattca tgatcgcatc
     2341 cccgagcgaa tgctctcctg aacggcctga atctgcgtcg agcacaacac ggaagggatc
     2401 ttcgtccgaa aaaccacgcc gtaaaacgtc aagcgcctct gtccgcgagg agacgacggc
     2461 tctcatgccc caggactcga actggcgcaa gaaaaaggct tgcgaggccg cgttgccgtc
     2521 gacgacgaga atacggagcc ccgccactcc ctcgggagct tccggagctt ccgcgccgcg
     2581 gccgtttttt tcgaagggaa gcacaaacca aaacgtagaa ccgtttccgt gttcactctc
     2641 cacaccgatg gaaccgccca tcctctccac aagatgcttc gatatggcca atccaagacc
     2701 tgtccctccg tactggcgag ttgtcgaagc gtcaagctgg gtgaacttgg tgaaaagaag
     2761 ccccattttc tctccgggga taccgattcc cgtatcccgc accgaaaaac ggatccacag
     2821 cgtttcctcg gtttcggact ctttcgagac agagatcacc gtctcgcctt cctgcgtgaa
     2881 cttcaccgcg ttgcttccca aattcatcag aatctgccgg atgcgcacgg gatctccgcg
     2941 caccttcgac ggaagcgacg gttcgacgcc gcaggcgagt tcgatcccct tcgtgtaggc
     3001 ctgaagggca agcgttcccg caaagccctc cacagtattt ctcaggtcga aatccacaac
     3061 ttccagttcg agctttcccg cttcaatctt tgcaaagtcg aggatttcgt tgataaggaa
     3121 gagcagcgtt tccgcgctcg ccctgacggt ctccgcctgg cggcgttgct cggaagaaag
     3181 cgtggagtcg agcagcagtt cggtaaaccc gatcacggcg ttgatcgggg tgcggatctc
     3241 gtggcttacg tttgcaagga aggcgctttt cgcctcgttt gccgcttccg ccttgagcac
     3301 catctcctgt gcgagctccg tctgccgttc cagttcacgg ttgctctcgc tcaattcctc
     3361 cgtccgcacc gcaaccaacc tgtccagttc gtcgcgatgc acgcgaagct cttcttcgat
     3421 ctttttcgtt tccgtaatat ccatcgcaat ctcaaggcgt acgtatttgc ggtcggtcca
     3481 gcgtatcgca gaatcgcggc agtcgtacca gcgcttcgta cgctcgttga atacctgcca
     3541 acgccgtact cccgccggtt ctccgtcgtc gccgagaagc ggagcttcgg cgcagaactc
     3601 gcacggcccc gtctgtcctt tttgaatagc ctcccagcat ctcctgccga cgacgtcgcc
     3661 gaagattttc ctgccgaaat cgttgatcat caaaatctcg aacgtgtcga catccgtcac
     3721 aaagacaagc gcgtcgatgc tgttgagtac tgccagaagg cgctgttcgc tctctttaac
     3781 tgtctgcgtc gcaaccttct ctttggtcac gtcgatatgc gctaccacag cccccccttc
     3841 ggggaagaga agcggcgtga cgcgcatcag gaaccagcgt ttctcgtcgg gcgagtggca
     3901 aggatactcc atctcgaacg attcggaacg gccggagagg accgatcgga ttccggccat
     3961 ggctctttgc gcgtctctgt agtcctccgt atccgaagaa caggcgcagg cggcgaagta
     4021 gttcgccccc gtatcgagcg tgcggaagtc cttcgcctgg ttctccctcg cgaatcttcg
     4081 ccacgtttcg ttgatcgcga gcatattccc ctct
//
LOCUS       DAOTIL010000144         4114 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminobacteriaceae bacterium isolate AMR_MDS_3026
            NODE_25737_length_4114_cov_3.666913, whole genome shotgun sequence.
ACCESSION   DAOTIL010000144 DAOTIL010000000
VERSION     DAOTIL010000144.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868050
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminobacteriaceae bacterium (wastewater metagenome)
  ORGANISM  Aminobacteriaceae bacterium
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae.
REFERENCE   1  (bases 1 to 4114)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4114)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:19:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,224
            CDSs (total)                      :: 3,198
            Genes (coding)                    :: 3,184
            CDSs (with protein)               :: 3,184
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4114
                     /organism="Aminobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_25737_length_4114_cov_3.666913"
                     /isolate="AMR_MDS_3026"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:3034609"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1675)
                     /locus_tag="P5201_04170"
     CDS             complement(<1..1675)
                     /locus_tag="P5201_04170"
                     /inference="COORDINATES: protein motif:HMM:NF012869.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine peptidase family C39 domain-containing
                     protein"
                     /protein_id="HRV97759.1"
                     /translation="MPDRPAAMKTPWKNRRVATPTMLQMEATECGAASLGIVLGYYGL
                     HLPLETLREECGVSRDGSRATNVLKAARRHGMEAKGYRKEPQTLGDLPLPIILFWNFN
                     HFLVLEGIKGDKVYLNDPAAGPRTVDRETLDRSFTGVAISIVPGETFRPEGRPRSPWA
                     ALARRLVGARAALLFAVLAGLALVIPGLMEPAFQQIFVDRLLIEGRLEWFRPMLTLMA
                     IVALVKGALTWLQGSVLLRQELAMAIRDSASFMNHLLKLPYTFFLQRYAGEIGNRVQL
                     NDRVAQLLSRELATTVLQCILVLFYAVMITLYDPPVSVIALGAAGACALLIVSVNRRR
                     TDLNERFTGEVGKFAGAAIGGLSVIETIKSFGGEEDQFARLAGYLSKIADCRIELSRL
                     AVRIQAVLPTLTALAAAGVLCFGGFRVMDGVLTMGMLLALQTLMTQTINPVTELVTLW
                     DSFQTAQGNLNRLDDVLRYPRMPERPALEDGAMASSKQAGARTVRLSGRLELRDVSFG
                     YSKLGEPLIQDFSLTLVPGSRVALVGGSGSGKSTVARIVCGLFEPWSGEV"
     gene            complement(1668..2963)
                     /locus_tag="P5201_04175"
     CDS             complement(1668..2963)
                     /locus_tag="P5201_04175"
                     /inference="COORDINATES: protein motif:HMM:TIGR03794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NHLP bacteriocin system secretion protein"
                     /protein_id="HRV97760.1"
                     /translation="MKKQIFRQSALDRLSSPEQLDMLFQVVRPAYWAALSALLLLLTV
                     LVVWGFYGTVTTMVSGQGILLREGARVDASSPGSGRLEVIFVDIDDPVEKGHVIARLA
                     QPDLRNQIAEMRGRVEILRAERAMGTEMGVDKKTLESEFLARRRQALNSSIKVARDRA
                     ESLREQLEQYDALYAKKYITRSQYLDVKDRYNAALQEILSFNEELARLPMTAADTTSQ
                     IERQRIDVDMRLLTAEKQLDALEERYDLETSVRSPETGRVLEIFKLEGQVISAGEALL
                     SLEKSDDSGAPLYVHAYVQPQDGKKILPEMEAQISPSVVKQEEFGVLRGRVTQVSSFP
                     ASARGMTRVLGNEQLVQTLSRGGAPIAVTMALSPSDRTESGYVWSSTDGPPLTLQSGT
                     LCGVTVVVRRQAPATLVLPFLKKFFLGVGEERDGGTKDA"
     gene            complement(3114..3377)
                     /locus_tag="P5201_04180"
     CDS             complement(3114..3377)
                     /locus_tag="P5201_04180"
                     /inference="COORDINATES: protein motif:HMM:TIGR03798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Nif11-like leader peptide family natural product
                     precursor"
                     /protein_id="HRV97761.1"
                     /translation="MSMESAVAFYERMEKDVELQRKLKELGSKERVQPYLKNELGYDF
                     TGEEMRKVILDKNPEMTDGDLEEVTGGAHYPIFGEISAIITGF"
     gene            complement(3480..3893)
                     /locus_tag="P5201_04185"
     CDS             complement(3480..3893)
                     /locus_tag="P5201_04185"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRV97762.1"
                     /translation="MSKENVRKFYEVLSKDADLQRRFREKNELLSGKYRDGAPGDERL
                     EGLFLKEILPVAREAGFEFSFQELQEYSADSKMTGELMDEELAAVAGGGDLCVCVVGG
                     YGTIGDVAIGCAMYGGAESSTFFCFCFMGGGGGLR"
     gene            complement(3926..>4114)
                     /locus_tag="P5201_04190"
     CDS             complement(3926..>4114)
                     /locus_tag="P5201_04190"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRV97763.1"
                     /translation="HRLSCGGGCRAGSLAAGNEYLGVDESTCAFFKNGYEERIRVAWG
                     GNDASGCAPGCGDFRRCT"
BASE COUNT          811 a         1402 c         1162 g          739 t
ORIGIN      
        1 gcacctcccc cgaccacggc tcgaacagac cgcagacaat gcgcgccacc gtggacttgc
       61 ccgacccgga accgcccaca agcgccacgc gggagccggg gacaagcgtc agcgaaaaat
      121 cctggatcag cggctcgccg agcttggagt agccgaagga gacgtcgcgc aactcaagcc
      181 gccctgagag acgcaccgta cgcgcccccg cctgcttcga cgaggccatc gccccgtcct
      241 ccagcgcggg tcgctcgggc atacgtggat agcggagcac atcgtcgagg cggttcaggt
      301 tcccctgcgc agtctggaag gagtcccaaa gagtcaccag ctccgtcacc gggttgatcg
      361 tctgcgtcat cagcgtctgc agcgcaagca gcatgcccat ggtgagcacg ccgtccatca
      421 ccctgaagcc gccgaagcag agcacccccg ccgcggcgag ggccgtcagc gtcggcagga
      481 cagcctgaat gcgtaccgca agacgggaaa gttcgatacg gcagtccgcg atcttgctta
      541 aatacccggc gagccgggcg aactggtcct cctccccgcc gaaggatttg atcgtctcaa
      601 tgacggagag gccgccgatc gcggctccgg cgaactttcc gacctcgccg gtgaagcgct
      661 cgttcagatc ggtccgccgc ctgttcaccg agacgatgag cagcgcgcag gcccccgcgg
      721 cgccgagagc gatcacggag accggaggat cgtaaagggt gatcatcaca gcgtagaaga
      781 gcacgaggat gcactggagc accgtcgtcg ccagctcgcg ggagagaagc tgcgccaccc
      841 ggtcgttgag ctgcacccgg ttgccgatct ccccggcgta ccgctgcaga aagaaggtgt
      901 aggggagctt cagcagatgg ttcatgaagg acgccgaatc gcggatcgcc atcgccagct
      961 cctgccgcag cagcaccgaa ccctggagcc acgtcagcgc ccccttgacg agcgcgacga
     1021 tcgccatgag cgtcagcatc gggcggaacc attcgaggcg tccctcgatg agaaggcggt
     1081 cgacgaagat ctgctggaag gctggttcca tgaggccggg tatcaccagc gcaagcccgg
     1141 cgagtacggc gaagagcaac gccgcacgag cgcccacaag ccgtcttgcg agcgcagccc
     1201 acgggcttcg cggacgtccc tccggacgaa aggtttctcc tggaacgatg gagatggcaa
     1261 caccggtgaa cgaacggtcc agagtctccc ggtccaccgt gcgcggtccg gccgcggggt
     1321 cgttcaggta gactttatcg cccttgatgc cttcgagcac aaggaagtga ttgaagttcc
     1381 agaaaagaat gattgggagg gggagatcgc ccagggtctg cggttccttg cggtacccct
     1441 tcgcctccat cccgtggcgc cgcgccgcct tcaggacgtt ggtcgcccgg ctgccgtcgc
     1501 ggctcacgcc gcactcctcg cgcagcgtct caagaggcag atggagcccg taatacccca
     1561 gaacgatccc cagcgaggcc gcgccgcact cggtggcctc catctggagc atcgtcggcg
     1621 tcgcgacgcg cctgtttttc cagggcgtct tcatcgccgc cgggcgatca ggcatctttc
     1681 gttcctccgt cgcgttcctc accgacgccg aggaagaact tcttcaaaaa cgggaggacg
     1741 agcgtcgccg gagcctgacg gcgcaccacc accgtcacgc cgcaaagagt gccgctctgg
     1801 agggtcagcg ggggcccgtc cgtggaggac cagacgtagc cgctctccgt ccggtcggac
     1861 ggggagaggg ccatcgtcac ggctatcggc gcgccgccgc gcgagagcgt ctgaacgagc
     1921 tgctcgttcc ccagcacccg cgtcatgccc ctcgcggacg cagggaatga ggagacctgc
     1981 gtcacccgtc cgcgaaggac gccgaactcc tcctgcttca ccacggacgg ggagatctgc
     2041 gcctccatct ccggcaggat cttcttgccg tcctgcggct gaacgtaggc gtgcacgtac
     2101 agcggcgcgc cggagtcgtc gctcttttca agcgagagca gggcttcccc cgcggagatg
     2161 acctgcccct ccagcttgaa aatttccagc acgcgcccgg tctcggggct gcgaacggaa
     2221 gtctccagat cgtagcgctc ctcgagcgcg tcaagctgtt tctccgccgt cagcaggcgc
     2281 atgtccacgt cgatccgctg ccgctctatc tgcgaagtag tgtcggcggc ggtcatcggc
     2341 agccgggcca gctcctcgtt gaaggagaga atctcctgaa gcgcggcgtt gtaccggtcc
     2401 ttcacatcca gatactgact ccgcgtgatg tacttcttcg catagagagc gtcgtactgc
     2461 tcgagctgct cgcggagcga ctcggcgcgg tcgcgcgcca ccttgatcga agagttcagc
     2521 gcctgtctgc gccgcgcgag gaactccgac tccagcgttt ttttatcgac gcccatctcc
     2581 gtccccatcg cccgctcggc acgcaaaatc tccaccctgc cccgcatctc ggcgatctgg
     2641 ttccgcagat cgggctgggc aagccgggca atcacatgcc ccttctcgac gggatcgtcg
     2701 atgtcgacga aaataacctc caggcggccg cttcccggag acgaggcgtc gacgcgcgcg
     2761 ccttcccgca acagaatacc ctgcccggag accatcgtcg tcacagtgcc gtagaacccc
     2821 cagacaacca gaacggtcaa caggagcagc agtgcggaga gcgccgccca gtacgcgggc
     2881 cggacgacct gaaagagcat gtcgagctgt tcgggggagg aaagcctgtc gagagccgac
     2941 tggcggaaaa tctgtttttt cacggtcggg cacgctcctt caggtaaaat cagaactgac
     3001 gtatcacggt atacgcggga gaattcctac tgcacagaat tatacacccc cgcttcaatg
     3061 agtgaaacgg gcattccagc gtattggaag cgccacctcc cccgggaaag cgatcagaat
     3121 ccggtaatga tggcggatat ctcaccgaaa atcgggtaat gcgctcctcc ggtcacctcc
     3181 tccaaatcgc cgtcggtcat ctcgggattc ttgtccaaaa tcaccttccg catctcctcc
     3241 ccggtgaagt catagcccaa ctcattcttg aggtatggct gcaccctctc cttgcttcca
     3301 agctccttca actttcgctg caattcgacg tccttctcca ttcgctcgta aaacgcaact
     3361 gcggattcca tcgacatcta caacccctcc ttcaaattcg tccatccctt gcggaacgga
     3421 cgttcccctg acttctccga atccctcctc gtaatcaact tttcaaggcc gttgcgatgc
     3481 tatctgagcc ctccgcctcc tcccataaag caaaagcaga aaaatgtaga tgactcggcc
     3541 cctccataca tcgcacatcc gatcgccacg tctccaatag taccgtatcc gcccactacg
     3601 cagacgcaca ggtcccctcc cccggcgacc gcggccagct cctcgtccat aagttcgccc
     3661 gtcattttgg aatcggccga gtactcctgc agctcctgaa acgagaattc aaaacccgct
     3721 tcccgggcaa ccggaagaat ttctttcagg aacagcccct ccaaacgttc atcgccgggc
     3781 gcgccatctc ggtactttcc tgagagcaat tcatttttct ccctgaatct ccgctgtaaa
     3841 tcagcatcct tcgacaacac ttcatagaac ttccgcacgt tctccttcga catctccagt
     3901 cacctctttc tgaacgatgt tcgtttcatg tgcacctccg gaaatcaccg cagccgggcg
     3961 cgcatccgga tgcgtcgttt ccgccccaag caacccgtat tctctcctcg tacccgtttt
     4021 tgaaaaaggc gcatgtcgac tcgtcgacgc cgagatattc gtttcctgcc gcgagggagc
     4081 ccgcgcggca acctccgccg caggaaaggc ggtg
//
LOCUS       DAOTIL010000145         4103 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminobacteriaceae bacterium isolate AMR_MDS_3026
            NODE_25847_length_4103_cov_2.920949, whole genome shotgun sequence.
ACCESSION   DAOTIL010000145 DAOTIL010000000
VERSION     DAOTIL010000145.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868050
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminobacteriaceae bacterium (wastewater metagenome)
  ORGANISM  Aminobacteriaceae bacterium
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae.
REFERENCE   1  (bases 1 to 4103)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4103)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:19:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,224
            CDSs (total)                      :: 3,198
            Genes (coding)                    :: 3,184
            CDSs (with protein)               :: 3,184
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4103
                     /organism="Aminobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_25847_length_4103_cov_2.920949"
                     /isolate="AMR_MDS_3026"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:3034609"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..642)
                     /locus_tag="P5201_04195"
     CDS             complement(<1..642)
                     /locus_tag="P5201_04195"
                     /inference="COORDINATES: protein motif:HMM:NF018513.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter large permease subunit"
                     /protein_id="HRV97764.1"
                     /translation="MLWLFLGILFFFTIFGVPIVFCLGIANIVMLLVMDIPLMALPAK
                     VIQGADSFPLLAIPFFMFVGEVMNRGGIARRLVDFADALVGHITGGLGHVSILSSMFF
                     GGICGSAVAETAAIGGLLIPPMKKQGFPAPLAAAICASAAVIGIIIPPSIPFILFGII
                     TSTSIARIFLGGIIPGIVVGLALMVTTYFITKKKGIARPAPDRKFSLKRVGRTL"
     gene            complement(647..1141)
                     /locus_tag="P5201_04200"
     CDS             complement(647..1141)
                     /locus_tag="P5201_04200"
                     /inference="COORDINATES: protein motif:HMM:NF016200.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter small permease"
                     /protein_id="HRV97765.1"
                     /translation="MKRLLQLGTALYRVALTVMTMALFIIVGVSVFTRYCLNSSLGWS
                     DELSRVIFIWVTFLGAAYAYGLDEHIGLDFVVDRIRSDRVRTVVRLLSEIAIGCVILV
                     ITWHGWEVAFSATNLSPALDIPMTWVYIVVPLTGCLMVVMNILKIGKWIGMLLATPSA
                     PAGR"
     gene            complement(1138..2427)
                     /gene="larA"
                     /locus_tag="P5201_04205"
     CDS             complement(1138..2427)
                     /gene="larA"
                     /locus_tag="P5201_04205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013049042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050043 - lactate racemase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nickel-dependent lactate racemase"
                     /protein_id="HRV97766.1"
                     /translation="MARIAFPYDGFGDMVIPDENLLAVLAPRTATPPTGTDLDEVRRA
                     LAEPIASPRLGEMVKQGESVLLLIDDNTRNTPAARILPLVLEEMAAGGVQIQDTAILI
                     ALGTHRHMTDAEVEAKVGAELSGRIRIFQHDAHDESGLAHIGVTEHGTDVWVNKLLLE
                     FDHVIAVGHITPHRVAGFSGGAKMVQPGVSGVVTTGQTHWISALYDGAEIMGTIDNPV
                     RREMNAVAKKAGLSFIVNVVLDREERIYRCVCGDPEKAHAAGCEASREIFGVPFPEYA
                     DIVVTDTFPADSELWQAAKGIYAGDLPLKRGGVLITVTPCPEGVAQGHPELTEIGYLP
                     FSEVKEMVDRNEIEDLTMAAHIVHVGRIIRDKGRGIMVCPCIDDATSKKLGFFPAATP
                     QQALDLAFSMTDPDATVIVLQNGGDLLPIHEPRRDAQ"
     gene            2809..4080
                     /locus_tag="P5201_04210"
     CDS             2809..4080
                     /locus_tag="P5201_04210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005659593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin family protein"
                     /protein_id="HRV97767.1"
                     /translation="MNQMSFFGFLALFLFCFVLSAFFSASEMAYSGINKIRVKRFVEE
                     GRPGAVKAQELLRDFNGTLSAILIGGNIVDIFMTALATAVLTTLFGPIGAVYSTLLMT
                     SLIILFGEILPKAFVKDRSEEFALSAAGMLHLLVRLFRPFTNFTRKLAADLRRWAPGP
                     ADNLPGVTHDELLSIVDAMHEEGVLPGAEQELIGNAISFNELEVWEVQTPRVDLFAIN
                     IREPLENVRNLVVKNHYSRVPVYEGTVDNIVGFLSEKDFLAKCVQGEPFELKSVLTRP
                     LLIAGSASLMDAFRILQASRSHMAVILDEYGGTSGIVTMEDLLEEIVGEISDEFDTEE
                     LPELEKIGKNSWDVDSRMPLADFSEEMGISLPVDEFDTIGGFVFDIFGKIPVKDECVK
                     YNDISFKIKDIDGTRINRILVTLPPRSRDGK"
BASE COUNT          815 a         1143 c         1278 g          867 t
ORIGIN      
        1 gagagtccgg ccgacgcgct tgagcgaaaa cttccggtcc ggagcgggtc tggcgatccc
       61 ctttttcttc gtgatgaagt aggtggtcac catcagggcc agtccgacga cgatgccggg
      121 gatgatgccg ccgaggaaga tgcgggcgat cgaggtgctc gtgatgatgc cgaagaggat
      181 gaaggggatg ctcggcggaa tgatgatgcc gatgaccgcc gccgacgcgc agatcgccgc
      241 cgcgagcggg gcggggaagc cctgcttctt catcggcggg atgagcagac cgccgatcgc
      301 cgccgtctcg gctaccgccg aaccgcagat gccgccgaag aacatgctgc tcaggatgga
      361 gacgtgcccc aggccgcccg tgatgtgccc cacgagggcg tccgcgaagt ccacaagcct
      421 gcgggcgatg ccgcccctgt tcatcacctc gcccacgaac atgaagaagg ggatggcgag
      481 gagcgggaag ctgtcggcgc cctggatgac cttcgcgggg agcgccatca gggggatgtc
      541 catcacgagc agcatcacga tgttcgcgat gcccaggcaa aagacgatgg gaacgccgaa
      601 gatggtaaag aagaagagaa tgccgagaaa gagccagagc atggcgttac ctccccgccg
      661 gggcggacgg cgttgcgagc agcatgccga tccacttccc gatcttcagg atgttcatga
      721 ccaccatcag gcaccccgta agcgggacga cgatgtagac ccaggtcatt gggatatcaa
      781 gcgcgggaga gaggttggtc gcggagaagg cgacctccca gccgtgccag gtgataacga
      841 ggatgacgca gccgatggcg atctcgctca ggaggcggac gacggtacgg actcggtcgc
      901 tcctgatgcg gtccacgacg aagtccagtc cgatgtgttc gtccagtccg taggcgtacg
      961 ccgccccgag gaaggtcacc cagatgaaga tgacgcgcga cagttcgtcc gaccatccga
     1021 gcgagctgtt gaggcagtag cgcgtgaaga cgctcacgcc gacgatgatg aagagggcca
     1081 ttgtcatgac ggtgagcgcg actctgtaga gggcggtccc cagttgcagc agccgcttca
     1141 ttgtgcgtcc ctccggggtt cgtgaatcgg gagcagatcg ccgccgttct gtaggacgat
     1201 caccgtagcg tccgggtcgg tcatcgagaa ggcgagatcg agcgcttgct gcggcgtcgc
     1261 ggcggggaaa aagccgagtt tcttcgaggt tgcgtcgtcg atgcaggggc agaccataat
     1321 cccccgcccc ttgtcgcgga tgatccgccc gacgtggacg atatgcgcgg ccatcgtcaa
     1381 gtcctcgatc tcgtttctgt ccaccatttc ctttacttcg gagaagggga ggtagccgat
     1441 ctccgtgagt tccgggtgcc cctgcgcgac gccctcgggg cagggggtga ccgtgattaa
     1501 aacgccgccg cgcttcaacg ggaggtctcc cgcgtagatc cccttcgcgg cctgccacag
     1561 ttcgctgtcg gcggggaatg tgtccgtaac cacgatgtcg gcgtactcgg ggaacggaac
     1621 gccgaagatc tcccgcgacg cctcgcatcc tgctgcgtgc gccttctccg ggtcgccgca
     1681 gacgcagcgg tagatacgct cctcccggtc aaggaccacg ttcacgatga aagagagccc
     1741 tgctttcttc gccacggcgt tcatctcgcg gcgcacgggg ttgtcgatgg tccccatgat
     1801 ctcagcgccg tcgtagagcg cgctgatcca gtgcgtctgc cctgtggtga ccactccgga
     1861 gacgcccggc tggaccatct tcgcccctcc ggagaaacct gcgaccctgt gcggggtgat
     1921 gtgtcccact gcgattacgt ggtcgaactc aagaagcagc ttgttcaccc agacgtcggt
     1981 accgtgttcg gtgaccccga tatgggccag cccgctctcg tcgtgggcgt cgtgctggaa
     2041 tatccgtatg cgcccggaga gttccgcgcc aacctttgcc tccacctcgg cgtccgtcat
     2101 gtggcggtgc gttccgaggg cgatcagtat cgcagtgtcc tgaatctgta cgccgcccgc
     2161 ggccatctct tccaggacga gcggaagtat gcgggccgcg ggggtgtttc gggtgttgtc
     2221 gtcgatgagc agcagaacgg attctccctg cttgaccatc tcacccagcc ttggggaggc
     2281 gatcggttcg gcaagagccc gtcgtacttc gtcaagatca gttcccgtcg ggggcgtcgc
     2341 agttcgggga gcgagcaccg ccagaaggtt ctcgtcggga atcaccatat cgccgaaacc
     2401 gtcataggga aaggcgattc gagccatggc gcacctcctt ctcgattttc gcggggatgt
     2461 cgtcttggta tattgcttcg cctccgaaaa atggcgcccg gaggagggag ttttgtcagg
     2521 cgaaacacta tcccgcgctt tttttccatg ttccattata atgccttttc agcgttttat
     2581 gcgcgaagag ggcgaaaaaa ttttgctata agaagaccgt atttcggaag gaagtttgct
     2641 cgtgttatga tgtgctccgg aggtgcgtac gatgtctgcg gcaaaaagaa gaaatgaaac
     2701 gcttgtgcat cttttggggc cgttcttctc tcttcctgcg attcgagcgt acggggagag
     2761 atgttctggg cagtgtgcgg aatgcatgcg cgcgggaggg gttgtgtcat gaatcaaatg
     2821 tcattctttg gttttctcgc cctctttctc ttctgttttg ttctctcggc ttttttttcc
     2881 gcttcggaga tggcatattc cggcatcaac aaaatcaggg tgaagcgctt cgtcgaggag
     2941 gggcgtccgg gcgcagtgaa ggctcaggag ctgctgcgcg atttcaacgg gacgctttcg
     3001 gcaattctca tcggcgggaa tatcgtcgac atctttatga cggcgttggc tactgccgtg
     3061 ctgacaacac ttttcggtcc cataggcgcg gtgtattcga ctcttttaat gacgtctctt
     3121 atcatcctct tcggcgaaat actgccgaaa gcgttcgtaa aagaccgctc cgaagagttt
     3181 gcgctctccg ccgcggggat gctgcatctt ctggtgcgcc ttttccgccc ctttacgaac
     3241 ttcacccgga agcttgcggc agacttacgg cgctgggcgc ccggtcctgc cgacaatctt
     3301 cccggcgtga cccacgacga gctgctctcg atcgtggatg cgatgcacga agagggagtg
     3361 ctgccggggg cggagcagga gctcatcggg aacgccatca gcttcaacga gttggaggtg
     3421 tgggaggtgc agacgccgcg agtggacctt ttcgccatca acatcaggga gcctctcgaa
     3481 aacgtccgca atctggtggt caaaaatcac tattccagag ttccggtcta cgagggcacg
     3541 gtggacaaca tcgtcggttt cctcagcgaa aaggattttc tcgccaagtg cgtacagggc
     3601 gaacctttcg aactgaagag cgtgcttacc cggcctcttc tcatcgccgg cagcgccagc
     3661 ctgatggacg ctttccgcat ccttcaggcg tcgcgaagcc acatggccgt cattctggac
     3721 gagtacggag gaacctccgg tatcgtcacc atggaggacc ttcttgagga gattgtcggg
     3781 gaaataagcg acgagttcga taccgaggaa ctgcctgaac tggaaaagat cgggaaaaat
     3841 tcatgggatg ttgattcacg gatgcccctt gctgatttta gtgaagaaat gggtatatcg
     3901 cttcctgttg atgagtttga tacaataggg ggattcgtgt tcgatatctt cgggaaaatt
     3961 cccgtgaaag atgaatgtgt gaagtataac gatatatcat tcaagataaa ggacattgac
     4021 gggacccgta ttaaccggat tcttgttaca ttgccgcccc gcagcagaga cggaaaataa
     4081 tcatttccaa tggaaatcca gaa
//
LOCUS       DAOTIL010000146         4087 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminobacteriaceae bacterium isolate AMR_MDS_3026
            NODE_25954_length_4087_cov_3.946181, whole genome shotgun sequence.
ACCESSION   DAOTIL010000146 DAOTIL010000000
VERSION     DAOTIL010000146.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868050
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminobacteriaceae bacterium (wastewater metagenome)
  ORGANISM  Aminobacteriaceae bacterium
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae.
REFERENCE   1  (bases 1 to 4087)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4087)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:19:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,224
            CDSs (total)                      :: 3,198
            Genes (coding)                    :: 3,184
            CDSs (with protein)               :: 3,184
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4087
                     /organism="Aminobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_25954_length_4087_cov_3.946181"
                     /isolate="AMR_MDS_3026"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:3034609"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..300
                     /locus_tag="P5201_04215"
     CDS             <1..300
                     /locus_tag="P5201_04215"
                     /inference="COORDINATES: protein motif:HMM:NF018979.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter TctB
                     family protein"
                     /protein_id="HRV97768.1"
                     /translation="LLALALLLQGYRRLKNGVPAVLPQGADFSTGRVFLVFGLCCAYA
                     LLLPWAGFFPSTALFLVIFSLMIGERRLWPIVLLSFLSPAAILLIFRHGLNVLLP"
     gene            313..1821
                     /locus_tag="P5201_04220"
     CDS             313..1821
                     /locus_tag="P5201_04220"
                     /inference="COORDINATES: protein motif:HMM:NF014073.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter permease"
                     /protein_id="HRV97769.1"
                     /translation="MDLVLSVVSQIDAVFVFFIFAGAVAGLFVGAIPGLSVTMATALL
                     VSITYTWSVKNALGVILGVFVSGVYAGAVSAILINIPGAPSSVVTTLDGYPMARKGEA
                     ALALWAATFHSFVGTVFGFLVLMFVAKPVTSFALTFSAMDYFLLALFGLTTIGTLTAK
                     NYIKGGICALLGLLLSMIGIDPFSGGVRLTFGWFDLQGGIPLIAALIGLFGFSEVLDQ
                     VARGAMNAIAAEAGKMRYCFREMLRFLPLSLRSSLIGVFIGALPGAGGPVASIIAYDS
                     ARKSVKNPSRPFGEGAVEGVIASEAANNACIGGALIPMLTLAVPGDAVTAVILAAFYV
                     HGLQPGPLLFVRTPELFSIVLAGGFIASVAMLILGMTVVPRLSKIVLVPKRILMPLVT
                     VLCVVGTYAVNSSMFDVGLMFCFGLAGFLMRSRGYPPAPLVLGLVLGEMMDSNLRRAI
                     NMALAGDNFFLSLFGRPTSVILAVLVILSVLAAVPGVKDRVLSVFSRRERVE"
     gene            complement(1862..3154)
                     /locus_tag="P5201_04225"
     CDS             complement(1862..3154)
                     /locus_tag="P5201_04225"
                     /inference="COORDINATES: protein motif:HMM:NF024240.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HRV97770.1"
                     /translation="MIQRHFKDVCCDILILGGGIAGAAAALAAAREGASVVLAEKSGR
                     LGGIATNGLVSTLCGCYSSLPGRRKIIGGFRDLLAAELHRTGGILEGVDRDTHRNDTC
                     DPELLACALDTLMQRHDVQLLFSSCVIGADFSERLLRSVEVVNTESGTRCSITPRLAV
                     DASGGGLLSRLADPASIVSGGEGGKLQAATMVFRMDGVDGDRARLVTKDEIRTLLAEA
                     RRNGDVVGERGNGVLTVLPGNSSAIVNANWVQANMSVPEDVTRGLVEGRKKALENARF
                     FRKYVPGFGNAVLSSIAASLGVREASRIKGKYVLSQEDVMEGRRFEDAISLGSWPMEY
                     HDYEQNRLVYLWSENDYDIPFRCLLPEGLDNVIAAGRNISVSSMAYASTRVMGPCMAI
                     GQAAGTATRLCLKMNAFPKEVDIHLLRKTLLNEGAILE"
     gene            3519..>4087
                     /locus_tag="P5201_04230"
     CDS             3519..>4087
                     /locus_tag="P5201_04230"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HRV97771.1"
                     /translation="MISTSRRMELEKELLEQFHLQEQIFSLVPVPLVMKDANSVYLHV
                     NQAFADFLGLPREQVLGKGPFDLFSHEFAQRIFEEDQEIIRNGVPATDLERRIKNGHG
                     KMVWLKSYKGPVFNSSGGVIGIVGGNMDITAVKNAEEALRKSEERWKFAVEGAGDGVS
                     EWNLETGDVFYSKQWKAMLGYKDDEIAPT"
BASE COUNT          754 a         1178 c         1203 g          952 t
ORIGIN      
        1 ctactcgccc ttgcgctgct cctgcaaggc taccgccgac taaaaaatgg cgtgcctgcg
       61 gtattgccgc agggggcgga tttctcgacg ggaagggtat ttctcgtttt cggcttatgt
      121 tgcgcctacg ctctcttgct tccctgggcg ggcttcttcc cctctacggc gctttttctc
      181 gtgattttct ccctgatgat cggtgagcgc cgtctttggc ctattgtgct cctttcgttt
      241 ctttctccgg cggcgattct cctcatcttt cgccacggtt taaacgtgct gcttccatag
      301 gaggaagaat caatggacct tgtgctctca gtcgtttctc aaatcgacgc cgtctttgtg
      361 ttctttattt tcgccggggc tgtagcgggg ctcttcgtgg gggctatccc cggcctttcc
      421 gtgacaatgg cgacggcgct ccttgtgtct attacctata catggagtgt gaagaacgcg
      481 ctcggagtca tcctcggggt ctttgtctcc ggggtgtacg caggcgcggt ctccgccatt
      541 ctgatcaata ttccgggggc tccttcctcg gtagtgacaa cgcttgacgg gtatccgatg
      601 gcccgcaaag gcgaggcggc gctcgctctc tgggcagcga ccttccactc cttcgtcggc
      661 accgtcttcg gctttctcgt cctgatgttc gtcgccaagc ctgtgacgag cttcgcgctt
      721 actttttccg cgatggacta ctttctgctg gcgcttttcg gcctgaccac gatcggcacg
      781 ctgacggcga agaactatat caaagggggc atctgcgctc ttctgggact gctgttgagc
      841 atgataggta tcgacccctt ctccggcggc gttcgtctga cgttcgggtg gttcgacctt
      901 cagggaggca ttcctctgat cgctgccctg atagggctct tcgggttttc ggaggtgctc
      961 gaccaggttg cgcgcggcgc aatgaacgcc atcgcggcgg aggcggggaa gatgcggtac
     1021 tgcttccgcg agatgcttcg cttcctcccg ctttcgcttc gttcctcgct catcggggtc
     1081 tttatcgggg cgctgcccgg agcggggggg cccgtcgctt cgatcatcgc ctacgactcg
     1141 gcgcgaaaga gcgtgaagaa cccgagccgt ccgttcggcg aaggcgccgt ggaaggggtc
     1201 atcgcgagcg aagcggcgaa caacgcctgc atcggagggg cgctgatacc gatgctcacg
     1261 cttgcagtgc cgggagacgc ggtgacggca gtcatcctcg cggcttttta cgtccacgga
     1321 ttgcagccgg gaccgctgct cttcgtccgg acgcctgaac tcttctccat cgtcctcgca
     1381 gggggattca tcgcctccgt cgccatgctg atcctcggga tgaccgtagt cccgcggctc
     1441 tcgaagatcg tcctcgtccc caagcgaatt ctgatgccgc tcgtgacggt gctctgcgtg
     1501 gtcgggacct atgcggtgaa ctccagcatg ttcgacgtgg gattgatgtt ctgcttcgga
     1561 ctggcggggt ttctgatgcg gagccggggg tatccgcccg cgccgctcgt cctcggtctc
     1621 gtgctggggg agatgatgga ctcgaatctt cggcgtgcga tcaatatggc gttggcgggg
     1681 gataactttt tcctctcgct cttcggcaga ccgacaagcg tgatcctcgc ggttttagtg
     1741 attctctccg ttctcgccgc cgtgccggga gtgaaggacc gggtgctctc agttttttca
     1801 agaagagagc gggtagaata aagcagaata atgaagggga ggccgaccag cctccccttc
     1861 attattccag aatcgcccct tcgttcagaa gcgtctttcg gagcaggtgt atgtccactt
     1921 ccttggggaa cgcattcatc ttcaggcaga ggcgcgtcgc cgttcccgca gcctggccga
     1981 tggccatgca cggtcccatc actcgggtgg aggcgtatgc catcgacgat accgagatgt
     2041 tccgccctgc tgcgatgacg ttgtccagcc cctcggggag cagacagcgg aacggaatgt
     2101 cgtaatcgtt ttcgctccac aggtagacga gcctgttctg ttcgtagtca tgatactcca
     2161 tgggccagga gcccagcgaa attgcgtctt cgaagcgccg cccctccatg acgtcctcct
     2221 gcgagaggac gtacttgccc ttgatacgcg acgcctcgcg cacgccaagt gaagccgcga
     2281 tggaggagag gaccgcgttc ccgaagcccg ggacgtactt ccggaagaaa cgggcgttct
     2341 caagcgcctt tttccggcct tccacaagcc cgcgcgtcac atcttccgga accgacatat
     2401 tggcttgcac ccagttggcg ttgacgatgg ccgagctgtt gccgggaagc acggtcaaga
     2461 cgccgttgcc gcgctccccg acgacgtcgc cgttccggcg ggcctcggcg agcagcgtgc
     2521 gtatctcgtc cttggtgacg agacgagccc tgtctccgtc gacgccgtcc atgcggaaga
     2581 ccatggttgc agcctggagt ttgcctcctt cgccgcccga gacgatggat gccgggtcgg
     2641 ccagccggga gaggaggcct cctccggagg cgtcgacggc cagacgggga gttatcgaac
     2701 accgcgtccc cgattccgtg ttgacgacct cgacggaacg aagaaggcgt tccgagaagt
     2761 ccgcgccgat gacgcatgag gagaacaaca gctgcacatc gtgacgctgc atcagagtgt
     2821 cgagggcgca cgcgagcaat tccggatcgc aggtgtcgtt tctgtgcgtg tctctgtcca
     2881 ccccttccag tatgcctccc gtacgatgaa gctccgcagc caggagatcg cggaatccgc
     2941 cgatgatttt gcgccttccc ggcagtgaag aatagcagcc gcagagcgtg ctcacaagac
     3001 cgttcgtcgc gatgccgcca agtcgcccgc tcttttccgc caggacgacc gacgctccct
     3061 cgcgggctgc tgccaaagct gccgccgcac ctgcgatgcc gcctcccagg ataagaatat
     3121 cgcagcaaac gtctttgaaa tggcgttgaa tcattatggc ctcctcattt cttcgcttcc
     3181 gaacagtttt acaggaagcg gcgattcttc agtcgagagg ttaagtgtaa cggatacgac
     3241 gtacgcgaag caatctcaga aaactttttt cagagaatca ggcaaattat ttttttgttt
     3301 ccccctttgt gatagtattg tttcattcac gcgactccgc agacttgaga aatcccgaga
     3361 aatacccggg ttttttgcat cgtttccaca cacaaggggg aaattattca gtgtcctgca
     3421 aaaaaagtac cacaccggac atggtgcttc aaaaaaaagg accggctcct cttcgtcaga
     3481 agacaaaaaa ggaattcgag tctttatccg gagaaccttt gatttcaacg tctcgccgga
     3541 tggagctgga gaaggagcta ttggagcagt tccatctgca ggaacaaatt ttttccctgg
     3601 ttcccgttcc cctggtcatg aaagatgcga attccgtcta tctccatgtc aatcaggcgt
     3661 tcgccgattt tcttgggctt cccagggaac aggtgcttgg taaaggaccc ttcgatcttt
     3721 tttctcacga gttcgcgcaa cgcattttcg aagaggatca ggaaatcata cgaaacggcg
     3781 ttcccgcgac cgatctggaa cgccgtatta agaacggcca cggaaagatg gtatggctga
     3841 aatcgtataa aggccctgtg ttcaacagca gcggcggcgt gatcggtatc gtcggaggca
     3901 acatggacat tacggcggtc aagaatgctg aagaggcgct ccgcaagagc gaagagcgat
     3961 ggaagttcgc cgtcgagggg gcgggcgacg gagtcagcga gtggaatctg gagaccggcg
     4021 atgtgtttta ttcgaaacag tggaaagcaa tgctcgggta taaggatgac gagattgcgc
     4081 cgacctt
//
LOCUS       DAOTIL010000147         4059 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminobacteriaceae bacterium isolate AMR_MDS_3026
            NODE_26189_length_4059_cov_3.522727, whole genome shotgun sequence.
ACCESSION   DAOTIL010000147 DAOTIL010000000
VERSION     DAOTIL010000147.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868050
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminobacteriaceae bacterium (wastewater metagenome)
  ORGANISM  Aminobacteriaceae bacterium
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae.
REFERENCE   1  (bases 1 to 4059)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4059)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:19:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,224
            CDSs (total)                      :: 3,198
            Genes (coding)                    :: 3,184
            CDSs (with protein)               :: 3,184
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4059
                     /organism="Aminobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_26189_length_4059_cov_3.522727"
                     /isolate="AMR_MDS_3026"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:3034609"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..476
                     /locus_tag="P5201_04235"
     CDS             <1..476
                     /locus_tag="P5201_04235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006301315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRV97772.1"
                     /translation="DELRRRGRYVKNRLNEVGRGKISLKYHEPEQSFMEGVFARGDRR
                     LAPVLLAAWRKGARFDGWTETFSFSRWMEAFEECGVDPHGYVSRERSPDEGLPWDHIS
                     AGVAKEFLWRERLRSREEKLSPDCREDGKCAACGAACLACGACFPPSQEGGAPRA"
     gene            469..1176
                     /locus_tag="P5201_04240"
     CDS             469..1176
                     /locus_tag="P5201_04240"
                     /inference="COORDINATES: protein motif:HMM:NF021610.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2344 domain-containing protein"
                     /protein_id="HRV97773.1"
                     /translation="MLRVRLTYAKRGRACFIPHIAIPSVFSRSGYRAGISFQLSEGFS
                     PRPRISLGPELPVGVPALSEPLEVRLLSFSADELIRWDRALPPGFFFTGACVVTGEPG
                     SAEAKSLNKWCEASSCILALREEPAGASPLESFLQEMKDLGDVLAFERAEPLGFRCVL
                     ENPAQRSPGFLVKALQKRGIIEGWPDIFILREAVGRLDMAEESGTAHVLPLTHPLSEE
                     FEQALEKVATGESVFSE"
     gene            1398..2912
                     /locus_tag="P5201_04245"
     CDS             1398..2912
                     /locus_tag="P5201_04245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013048012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004540 - ribonuclease activity [Evidence
                     IEA];
                     GO_process: GO:0006401 - RNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rne/Rng family ribonuclease"
                     /protein_id="HRV97774.1"
                     /translation="MRGGSVAGNADRKIIANCIDPEETRVAIIEDGRLADLFVERMWE
                     RQKAGEIYKARVESVLPGIHASFVNLGDGRNAFLYLNDARGLDLQPNKELLVQVTKTA
                     RKNKGARVTSRISLPGRYVVLVPGGQESGVSKRIVDENERKRLKAIARELRGDYGIIV
                     RTAAEGVDDESLAHDVETLLALWREIEHTGRTQSAPCLLYRDLGLLGRVLRDELTEQV
                     SEIIVDGPEEFENVGSHLSSLCGGEPPELSLYKGTIPLFEHYGVEKEIDAALERKVWL
                     KSGAYLIIDHTEALTVIDVNTGKYIGKTDLRHTVLETNLEAASEIARQLRLRAIGGIV
                     VIDFIDMDFEEDRSRLLRRLEEVFQPDRYRARVFGVSQLGLVEITRKRARPDIRSMLT
                     RSCPACGGGGWVFREDTIAMAIKRFLRKVSFANRAEALLLEANAAVAQYIFETYLMLW
                     EAELGRKIFIAAAPDFAWSKFRLEAQGGVDQVERRIEQMEKREARIIVYRTSSS"
     gene            2887..>4059
                     /locus_tag="P5201_04250"
     CDS             2887..>4059
                     /locus_tag="P5201_04250"
                     /inference="COORDINATES: protein motif:HMM:NF024867.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HRV97775.1"
                     /translation="MYIERLHLKGFKSFGASLDLLLSPGFTAIVGPNGSGKSNLLDAL
                     RWVLGEAGSQRLRIVKQGDLLFSGSVTLPPASRAEVSLLLRSTPHSGRESAHSPEAGQ
                     CVLRRSYSSETGTVLTVDGTRVRLSDLDEVKRLWHLEGDQFAFIGQGEVAEAIHQRPA
                     QRRAHLEVLFGIDQYRKKRNETSQKLVAASEEVLRLGALAAELTHRREEIAPAVEIAQ
                     AAKKITDELEEKRRGYYFFRRRLLEEKSAALGEDLRTLREEEALRLRWKELWEAMYTR
                     SVQQCGEQEERVKRLLGDRDELVARREGLRRSCFAGAASIREILSRRRALAEEQKETA
                     ARLASLTKESEELKSKEASLHVEHGSRASERELLRKKVRELHEAIDAERRRKDETTN"
BASE COUNT          806 a         1175 c         1357 g          721 t
ORIGIN      
        1 tggacgagct gcggcggagg gggcggtacg tcaagaaccg gctcaacgaa gtcggccggg
       61 gaaagatttc cctgaagtac cacgaacccg agcagtcctt catggagggg gtcttcgccc
      121 gcggcgacag acggctggcg cctgtgctcc ttgccgcatg gaggaagggc gcgcgcttcg
      181 acggatggac cgagacgttt tctttttccc ggtggatgga ggccttcgaa gagtgcggtg
      241 tggatccgca cgggtacgtc tccagggagc gctcgccgga cgaggggctt ccctgggatc
      301 acatctccgc cggcgtcgcg aaggagttcc tctggaggga gcgtctgcgc tcccgcgaag
      361 agaaactctc tccggactgc cgcgaggacg gaaagtgcgc cgcgtgcggc gccgcctgcc
      421 tcgcttgcgg ggcatgtttt ccgccctctc aggaaggggg cgcgccccgt gcttagagtt
      481 cgcctgacct acgcgaagcg gggaagagcg tgcttcattc cgcatatcgc cataccttcg
      541 gtcttctcca gaagcggata ccgcgctgga atctccttcc agctttcgga ggggttttcc
      601 ccgcggccgc gcatcagcct cggcccggag ctccctgtgg gtgttcccgc gctgagcgag
      661 ccgctggaag tccgtttgct ctccttttcg gcggacgaac ttatcaggtg ggacagagcg
      721 cttcccccgg gttttttctt cacaggggcg tgcgttgtga ccggagaacc cgggtctgcg
      781 gaggcgaagt cgctgaacaa gtggtgcgag gcttcctcgt gcatcctcgc tctccgggag
      841 gagcctgccg gcgcatcgcc gctggaatcc ttcctgcagg agatgaagga cctgggggac
      901 gtgcttgctt tcgagcgggc ggagcccttg ggcttcaggt gcgttctgga gaaccccgcg
      961 caaaggagtc ccggcttcct ggtgaaggcg ctccaaaaaa gaggtatcat agaggggtgg
     1021 cccgatatct tcatcctgag ggaagcggtg gggcggcttg acatggcgga agagagcgga
     1081 acggcgcacg tgcttccgct gacgcatcct ctctccgagg agttcgagca ggcgcttgaa
     1141 aaggttgcaa ccggggagtc cgtattttca gagtgaggtt ccccggagta tagataaaga
     1201 aagcggcggg aattttctcc ttctcatgaa aaagagaagg agattcgtcc tgttgtacat
     1261 gtttgtccga aaagaggagg gagttgcgtt tgtcagagaa tgcggaggcc agaagcgaac
     1321 gcgcggaatc gcgcgacgct gtgatacccc gtaccgaagc gagggaggac gtcgatccgc
     1381 aggagggaag gcccggtttg cggggagggt ccgtcgccgg caacgccgac aggaagatta
     1441 tcgcgaactg catcgacccc gaggaaacgc gggtagccat catcgaggac ggccgtttag
     1501 ccgacctgtt cgtcgagcgc atgtgggagc gtcagaaggc gggagagatc tacaaggcca
     1561 gggttgagag cgtgctcccc ggcatccacg cttccttcgt caaccttggc gacgggagaa
     1621 acgccttcct ctacctcaac gacgccagag ggcttgatct ccaaccgaac aaggaacttc
     1681 tcgtccaggt gaccaagacc gccaggaaga acaagggggc gagggtgacc tccagaatct
     1741 ccctccccgg gcggtacgtc gtgctcgttc ccggcgggca ggagtccgga gtctccaagc
     1801 ggatcgtcga tgagaacgag cgaaaacgcc tgaaggccat cgcccgcgag cttcgcggcg
     1861 actacggtat tatcgtccgg acagctgcgg aaggggtgga cgacgaatcg ctcgcccacg
     1921 atgtggagac gctgcttgct ctgtggcgcg agatagagca tacggggcgg acccagagcg
     1981 ctccctgcct tctgtacaga gatctcggcc tgctggggag agtcctgcgg gacgagctca
     2041 ccgaacaggt ttcggagatc attgtggacg gccccgaaga gttcgagaac gtcggcagtc
     2101 acctttcgag cctgtgcggg ggcgaacccc cggagctttc gctgtacaag ggaacaatcc
     2161 cgctcttcga gcactacgga gtggagaagg agatcgacgc cgccctcgaa cgcaaggtgt
     2221 ggctcaaatc gggcgcttac ctgatcatcg accacacaga ggcgctgacc gttatcgacg
     2281 tcaacaccgg taagtatatc ggcaagacgg atcttcgcca taccgtcctg gagacgaatc
     2341 ttgaagccgc gtcggaaatt gcgcgtcagc tcaggctccg ggccatcggc ggcatcgtcg
     2401 tcatcgactt catcgacatg gactttgaag aggaccgttc ccgtctgctc aggaggctgg
     2461 aggaggtctt ccagcccgac agataccgcg cgcgcgtttt cggcgtctcg cagctcggac
     2521 ttgtagagat cacgcgcaaa cgagctcgtc ccgacatccg ctcgatgctc acccggagct
     2581 gccccgcgtg cggcggaggc ggatgggtct tccgcgagga taccatcgcg atggctatta
     2641 agcggttcct caggaaggtc tccttcgcca acagggcgga ggcgctgctt cttgaagcca
     2701 acgccgcggt tgcccagtat atcttcgaga cctacctcat gctctgggag gcggaactcg
     2761 gacggaagat cttcatcgcc gccgccccgg acttcgcgtg gagcaaattc cgccttgagg
     2821 cgcaaggggg cgtcgaccag gtggagcgtc gtatagaaca gatggagaag cgggaggcac
     2881 gaatcattgt atatcgaacg tcttcatctt aaaggcttca agagcttcgg agcctctctg
     2941 gatctcctcc tctcaccagg attcacggca atcgtcggtc ccaacggaag cggcaagagc
     3001 aacctgctcg acgcgctccg gtgggtgctg ggcgaagccg gttcgcaacg cctccgcatc
     3061 gtcaaacagg gggatcttct cttctccggg agcgtgacgc tgcctccggc ttcccgcgcc
     3121 gaggtctctc ttctgttgcg ctcgacgccg cactcgggcc gggagagtgc gcactctccg
     3181 gaggccggtc agtgcgtgct gcgacggagc tattcttccg aaacagggac ggtgctcacc
     3241 gtcgacggaa cgcgcgtccg gctttcggat ctcgacgagg tgaagcgtct ctggcatctc
     3301 gaaggagatc agtttgcctt catcgggcag ggagaggtcg ccgaggcgat ccatcaacgc
     3361 cccgcgcagc gacgcgcgca ccttgaggtg ctcttcggca tcgaccagta ccggaaaaag
     3421 agaaacgaaa cgtcgcagaa gctcgtcgcc gcatccgagg aggttctgcg tctcggcgcg
     3481 ctcgccgcgg agttgacgca ccgcagggag gagattgctc cggccgtaga aatcgcgcaa
     3541 gccgcgaaga agatcaccga cgaactggaa gaaaagaggc gggggtacta cttcttcaga
     3601 cgccgccttc tcgaggagaa gtctgcggcc ttgggagaag atctgcgcac gctcagggag
     3661 gaggaagccc tccggcttcg ctggaaagag ctgtgggagg cgatgtacac acggtcggtc
     3721 caacagtgcg gggagcagga agaacgggtg aagcggctcc tcggcgaccg cgacgaactc
     3781 gttgcgcgac gcgaaggact caggaggagc tgctttgcag gggcggcctc catacgggaa
     3841 atcctgtcgc ggcggcgcgc cctcgccgag gaacagaaag agactgcggc gcgccttgca
     3901 tcgttgacga aagagagcga ggagctgaag agcaaagagg cttctctgca cgtcgagcac
     3961 ggatcgcgtg cttcggagcg cgaactgctc cgaaagaagg tgcgcgagct gcacgaagcg
     4021 atcgacgcgg agcgcaggcg aaaggacgag accacgaac
//
LOCUS       DAOTIL010000148         4047 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminobacteriaceae bacterium isolate AMR_MDS_3026
            NODE_26285_length_4047_cov_3.583417, whole genome shotgun sequence.
ACCESSION   DAOTIL010000148 DAOTIL010000000
VERSION     DAOTIL010000148.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868050
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminobacteriaceae bacterium (wastewater metagenome)
  ORGANISM  Aminobacteriaceae bacterium
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae.
REFERENCE   1  (bases 1 to 4047)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4047)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:19:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,224
            CDSs (total)                      :: 3,198
            Genes (coding)                    :: 3,184
            CDSs (with protein)               :: 3,184
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4047
                     /organism="Aminobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_26285_length_4047_cov_3.583417"
                     /isolate="AMR_MDS_3026"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:3034609"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..883)
                     /locus_tag="P5201_04255"
     CDS             complement(<1..883)
                     /locus_tag="P5201_04255"
                     /inference="COORDINATES: protein motif:HMM:NF012562.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate isomerase"
                     /protein_id="HRV97776.1"
                     /translation="MTRGTRSGSDLLTVSFGMAVDSGADSASTRGVSVEALKECGDSL
                     AKADAWLREGRNEGRDGVGWLSLPYKDIGDIQEAAGWLRGFNAVVQAGIGGSALGNLM
                     LHGALLPPYWNELPEAKRGGPRFYLADNVDPRDNRTVWNLIEPEKTAFIVISKSGSTA
                     ETMANFLFFWDRLKVELGPKRAAEHVLVITDEEKGVLRPFADEIGCRRLVLPSDVGGR
                     FSVLSSVGLLSACALGIDGRKLLAGAAEMDEKLAQRASLFENPAWLLSALSVAHYPRG
                     RNMTVMMPYEDALERFCE"
     gene            complement(909..1145)
                     /locus_tag="P5201_04260"
     CDS             complement(909..1145)
                     /locus_tag="P5201_04260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013048747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015035 - protein-disulfide reductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin domain-containing protein"
                     /protein_id="HRV97777.1"
                     /translation="MITVYSTKTCPWCTKVKEYLEGKKIPFEVIDVASDREAAMEMVK
                     KTGQMGVPVTRIGEKYIVGYNPEAIDEEIAQQNQ"
     gene            1327..1836
                     /gene="nuoE"
                     /locus_tag="P5201_04265"
     CDS             1327..1836
                     /gene="nuoE"
                     /locus_tag="P5201_04265"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014163394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoE"
                     /protein_id="HRV97778.1"
                     /translation="MSTEASVSSALEQRLLPIADRYRGRKGITIPLLADLQKEMGYIA
                     PEAVDFIAHELDIPAAEMFGVATFYAMFRLQPEGKHVIRLCRGTACHVQGSARIGEQI
                     QRHLSVSDGGTTSDGVFTLQFVACLGCCSLAPVMMVDGEVFGLLTPEKAVDVLESVRS
                     ASSGKGAAD"
     gene            1855..3621
                     /locus_tag="P5201_04270"
     CDS             1855..3621
                     /locus_tag="P5201_04270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014163395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-ubiquinone oxidoreductase-F iron-sulfur
                     binding region domain-containing protein"
                     /protein_id="HRV97779.1"
                     /translation="MTKTIVKVGLASCGVAAGAVPIYETLLSALKGHDDVEIKKVGCI
                     GLCYMEPIVEIERDGKSIAYGKVDTELAKEIAESHALRGEIVQRAVILDPSSKACENE
                     RMDSQVRIVLRNSGLIDPEKIDEYVARDGYGALKKALSMTPEQVIEEVLASGLRGRGG
                     AGFPTGLKWKFARGAKGDKKYFVCNADEGDPGAFMDRSVLESDPHSIIEGMTIGGYSI
                     GSDEGIIYCRAEYPLAIKHLNVALEQAREKGYLGKNILGSNFSFDIHIKEGAGAFVCG
                     EETAMIASIEGRRGMPRPRPPFPANSGVFGKPTNINNVETIANIPWIILNGAAKFSEY
                     GIGKSRGTKVFALAGKIKKGGLAEVPMGMPLREVIFNVAGGIAENKKFKAVQLGGPSG
                     GCIPESLLDTPVDYESINATGAIMGSGGMVVMDETTCIVDVAKFFLAFVQKESCGKCP
                     FCRIGTKRMLEILERISEGKGVMEDLDTLLDLAYQVKEGSLCGLGQTAPNPVLTTLKY
                     FREEYEAHIRDKKCPAKVCTSLIHYVIDPAVCIGCTKCARNCPVSCIAGEVKKPHVID
                     DEKCIRCGQCKKVCPVGAISVE"
     gene            3649..>4047
                     /locus_tag="P5201_04275"
     CDS             3649..>4047
                     /locus_tag="P5201_04275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009200480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2Fe-2S iron-sulfur cluster-binding protein"
                     /protein_id="HRV97780.1"
                     /translation="MKKNIKVTLNGKTVYGYEGQRILDLCTECGVEIPTLCYDPHLSL
                     HGGCSVCLVEVEGAKTLLRACANTISQGMIIKTETERAVSARRTALELLLSDHVGDCR
                     PPCTLTCPANGNVQAYINLAAQGKYDESLDV"
BASE COUNT          905 a         1162 c         1139 g          841 t
ORIGIN      
        1 actcgcaaaa gcgttcgagc gcgtcttcgt acggcatcat gaccgtcatg ttgcgtccac
       61 gcgggtagtg cgccactgag agggcggaaa gaagccatgc cgggttctcg aaaagagaag
      121 cgcgctgcgc gagcttctcg tccatctctg ccgctcctgc gaggagtttt ctgccgtcga
      181 tcccgagtgc gcaagcggaa agaagcccta ctgaagagag caccgaaaag cgcccgccga
      241 catcggaagg aagtacaaga cgacgacagc cgatttcgtc ggcaaaaggc ctcagaacgc
      301 ccttttcttc atcggtgatg acaagaacat gctcagcagc tcttttcggc cccagctcca
      361 ccttcagccg atcccaaaag aaaagaaaat tcgccatcgt ctcggcggtg ctcccggatt
      421 tgctgatgac gatgaaggct gtcttctcag gctcgataag attccagacc gtcctgttgt
      481 cccgtggatc gacgttgtcc gcaagataga atctgggacc gccccgtttt gcctcgggca
      541 gttcgttcca atacggggga agaagcgctc cgtgcagcat cagattaccg agagcggaac
      601 cgccgattcc cgcctggacc actgcattga agccacgcaa ccatcctgcg gcttcttgta
      661 tatcgccgat atccttgtag ggaagggaaa gccaccctac tccgtccctt ccctcgttac
      721 gtccttcccg aagccaggcg tcggctttcg ccagactatc gccgcactct ttcagagctt
      781 cgacgctcac accgcgggta gacgccgaat ccgccccgct gtcaaccgcc attccaaatg
      841 atacagtaag cagatcggag cctgaacgtg ttcctctcgt cactttctcc actccttcct
      901 gtaacggctt actgattctg ttgcgctatc tcctcgtcga tggcctccgg attgtaacct
      961 acaatatact tttcccctat cctcgtcacc ggaacgccca tctgccctgt ttttttgacc
     1021 atctccatgg ccgcttcccg gtctgaagcg acgtcgatca cctcaaacgg tatttttttc
     1081 ccttccaggt actctttcac cttggtgcac cacgggcatg tcttcgtcga atagactgtt
     1141 atcatgacag acacctccat aaatgatata tacttgaggg gagtatatat caaatctcac
     1201 tcccagtcga gatttattat ataccgaatt tctttttgga tgggaaattg tcctcaatac
     1261 gcggtataat aaataccatc accggggtat ttcactgaaa aagcacgagg aggagagaaa
     1321 atgaccatgt cgacggaggc ctccgtgagt tccgctttgg agcagcggct tcttccaata
     1381 gcggaccgat accggggaag gaaggggatc accataccgc ttcttgcgga tctccagaag
     1441 gagatggggt acatcgctcc ggaagcggtg gattttattg cacacgagct ggatattcct
     1501 gctgcggaga tgttcggcgt cgccacattt tacgccatgt tccgcctgca gccggaggga
     1561 aagcatgtga tccgcctttg ccgggggacg gcatgtcacg ttcagggatc ggcgcgtata
     1621 ggggagcaga ttcagcgcca tctttcggtt tccgacggcg ggaccacctc cgacggagtg
     1681 ttcacacttc agttcgtcgc ctgtctggga tgctgcagcc tggcgccggt catgatggtg
     1741 gacggtgaag ttttcgggct gctcacgccc gaaaaagccg tcgatgttct tgaatccgtg
     1801 cgcagcgcct cctcgggaaa aggcgctgcg gactagctca tggaggtgtg caaggtgacc
     1861 aaaaccatcg tgaaggtcgg tctggccagc tgcggcgtgg ctgccggcgc cgtcccgata
     1921 tatgaaactc ttctctcggc cctgaaagga cacgacgacg tcgagataaa aaaagtcggc
     1981 tgcataggac tctgctacat ggagcctatc gttgaaattg aacgtgatgg gaaatccatc
     2041 gcctacggta aggtggatac cgagctcgcg aaggagatcg ccgagtcgca cgcgcttcgc
     2101 ggagagattg ttcagcgtgc ggtgattctc gatccttcat ccaaagcctg tgaaaacgag
     2161 cgtatggaca gtcaggtacg tatcgttctc aggaacagcg gactcatcga cccggaaaag
     2221 atcgacgagt atgtcgcccg cgacggctac ggcgcattga aaaaggcgct gtccatgacg
     2281 ccggagcagg taatcgaaga ggttctcgcc agcggcctcc gcgggcgcgg cggcgcggga
     2341 ttccccacag gactcaaatg gaagttcgct cgcggcgcca agggggacaa aaaatacttc
     2401 gtctgcaacg ccgacgaggg cgacccaggc gcgttcatgg accgctccgt tctggagagc
     2461 gatccgcaca gcatcatcga gggaatgacc atcggcggct actccatcgg ttcggacgag
     2521 gggatcatct actgcagagc ggagtacccg ctcgccatca agcacctgaa tgtcgcgctc
     2581 gaacaggcgc gtgaaaaagg gtacctcggg aagaacattt tggggtcgaa cttctccttc
     2641 gacattcaca taaaagaagg cgcgggcgcc tttgtatgcg gcgaggagac tgccatgatc
     2701 gcttccatcg aaggccggcg cggcatgccg cggccgcggc ctccgttccc tgcgaacagc
     2761 ggcgtcttcg gcaaaccgac gaatatcaac aacgtggaga ccatcgcgaa tattccctgg
     2821 atcattctga acggagcggc gaagttctcc gaatacggaa tcggcaagag ccgggggaca
     2881 aaagtcttcg ccctcgcagg aaaaatcaag aaaggcggcc tcgcggaggt tccgatggga
     2941 atgccgctgc gcgaggtcat cttcaacgtg gcgggcggta tcgcagaaaa caagaagttc
     3001 aaggccgtcc agcttggagg tccttcggga ggctgtattc ccgaatcgct gctcgacacc
     3061 cctgttgact atgaatccat caacgccacg ggcgccatta tgggctccgg cggaatggtg
     3121 gtcatggacg aaacgacctg catcgtcgac gttgccaagt tcttcctcgc cttcgttcag
     3181 aaggagtcct gcgggaagtg ccccttctgc cggatcggga cgaagcggat gcttgagatc
     3241 ctggaacgca tctccgaggg gaagggcgtc atggaagacc ttgatacgct ccttgatctt
     3301 gcctatcagg taaaggaggg ctctctctgc ggtcttggcc agacagctcc gaaccccgtg
     3361 cttaccacgc tgaaatactt ccgcgaagag tacgaggcac acatcaggga taagaagtgc
     3421 cccgcgaagg tctgtacgag tctcattcac tacgtgatcg accccgccgt gtgcatcggc
     3481 tgcacaaagt gcgcccggaa ctgtcctgtc tcctgtatcg ccggagaagt aaaaaagccc
     3541 cacgttatcg acgacgaaaa gtgcatccgg tgcggtcagt gtaaaaaggt ctgccctgtg
     3601 ggcgccattt ccgtggaata agcattgtcg ggaggatttc gagctgccat gaagaaaaac
     3661 ataaaagtca cactcaacgg aaaaactgtc tacggctacg aggggcaacg gattctcgac
     3721 ctctgtaccg agtgcggcgt ggagattccc acgctctgct acgatccgca tctgagcctg
     3781 cacggaggct gttctgtctg tctcgtcgaa gtcgaaggtg cgaagacgct cctgcgcgcg
     3841 tgcgccaaca ccatctcgca gggaatgatc ataaaaaccg agactgagcg tgcagtcagc
     3901 gcgcgccgta ccgcactcga gctgctgctc tccgaccacg tgggggactg ccgtcctcca
     3961 tgtacgctca cctgccctgc gaacggcaac gtgcaggcct atatcaatct cgccgcgcag
     4021 ggaaagtacg acgagtcgct cgacgtg
//
LOCUS       DAOTIL010000149         4039 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminobacteriaceae bacterium isolate AMR_MDS_3026
            NODE_26343_length_4039_cov_3.491215, whole genome shotgun sequence.
ACCESSION   DAOTIL010000149 DAOTIL010000000
VERSION     DAOTIL010000149.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868050
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminobacteriaceae bacterium (wastewater metagenome)
  ORGANISM  Aminobacteriaceae bacterium
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae.
REFERENCE   1  (bases 1 to 4039)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4039)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:19:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,224
            CDSs (total)                      :: 3,198
            Genes (coding)                    :: 3,184
            CDSs (with protein)               :: 3,184
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4039
                     /organism="Aminobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_26343_length_4039_cov_3.491215"
                     /isolate="AMR_MDS_3026"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:3034609"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1136)
                     /locus_tag="P5201_04280"
     CDS             complement(<1..1136)
                     /locus_tag="P5201_04280"
                     /inference="COORDINATES: protein motif:HMM:NF025019.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HRV97781.1"
                     /translation="MYIPRKMEKTALQLMEGFPCLAVTGARQSGKTTLARYLFPEKPY
                     VSLEDPDQREFAENDPRSFLDRFPDGALFDEAQRVPSLFSYLQGRIDLDGRMGLFVLT
                     GSQHFHLLESITQSLAGRTALLQLPPFSFDELRSAGLLPESADEAILSGGYPPLHTRT
                     VERTAWFANYTATYLERDVRSMLNVRDLSTFQRFLRLCSGRAGQLVNFSSLAGECGVV
                     HNTAKAWLSILEAGSIVFLVRPYFRNFNKRLLQTPKLYFHDTGLLCHLLGIRNEEQLE
                     THPLRGAVFENMVAVELLKARLNEGREPELYFWRDRSGFEIDFLIENDGLLDAIEVKS
                     GKTVTREFFRAIGRWKEIAGESAGKTFLIYGGDDEYEREGHSVLS"
     gene            1374..1652
                     /locus_tag="P5201_04285"
     CDS             1374..1652
                     /locus_tag="P5201_04285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008708795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0110001 - toxin-antitoxin complex
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system Phd/YefM family
                     antitoxin"
                     /protein_id="HRV97782.1"
                     /translation="MPHIRPVSDLRNKFAEISKIVHECQEPIFLTKNGYGDMVVMSIE
                     QFERLQFENEIVFKLREAENQSSADDRRYSHADVFDALRERLADGRKI"
     gene            1636..1959
                     /locus_tag="P5201_04290"
     CDS             1636..1959
                     /locus_tag="P5201_04290"
                     /inference="COORDINATES: protein motif:HMM:NF016876.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008657 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) inhibitor activity
                     [Evidence IEA];
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA];
                     GO_process: GO:0030541 - plasmid partitioning [Evidence
                     IEA];
                     GO_process: GO:0051290 - protein heterotetramerization
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HRV97783.1"
                     /translation="MGEKYNIRYLELARSDLLGIAEYVNSQAWERDAANRLVDSLEKA
                     ILRLESFPYSGALYGKSFGFKEEHRMLVVGNYLVFYVVYDDFVEIRRVIHGKRRFFDL
                     LQPLS"
     gene            complement(2013..3104)
                     /locus_tag="P5201_04295"
     CDS             complement(2013..3104)
                     /locus_tag="P5201_04295"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRV97784.1"
                     /translation="MKKLYGTLLLSLCLLLLSNAAGNAAETLDNRLAELRGIENRGDL
                     DHLGLEKLRTLGGTEPKDILSLPQADILWLLSYGLDMYDSMLYSLVGKSERGARRQDE
                     KLGEFTDRFNTIWNTINSAYTALSEKSTEPSPADKLFDLLKKGASPQELLPVTTEGLK
                     YTWEIVKGWFAESEKEEAFLTELKTLRAGNAALLENAPDSPWNFALLSYARLRRQSAD
                     YVKYLNQFEIDVARHSVPYEKTRVLNDGILSIKQELAAYDAKLRKAVVDIPVFTVQFY
                     SKARIDSAKGIFSDDTAAVLEAGALFARTAPLINLAVDDNVRKGWTEIAGALKDLLNE
                     EQYDELKSISQAVNMMWDFDDVPRTINWP"
     gene            complement(3191..3436)
                     /locus_tag="P5201_04300"
     CDS             complement(3191..3436)
                     /locus_tag="P5201_04300"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRV97785.1"
                     /translation="MNDSKTLAILLEFVQSPLVEGNYHIYKFDPQRVEKSDPELAEQI
                     VRLGEATERALEHLAENGIHVIAGGALPPYLTSSMLN"
     gene            3742..>4039
                     /locus_tag="P5201_04305"
     CDS             3742..>4039
                     /locus_tag="P5201_04305"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRV97786.1"
                     /translation="MTNGARKNVRSTWSGSADSSARVLGQSAYLLFRKVDEFGDEGKR
                     HALIQHAPDRLELRQFDAFFASFFPTFLPTFFSPLFSSFFFEFPDFLLQIGQHFV"
BASE COUNT          781 a         1091 c         1212 g          955 t
ORIGIN      
        1 gagaggaccg aatgcccttc cctttcgtac tcatcgtctc cgccgtagat caggaaggtc
       61 tttccggcgc tctcgccggc gatctccttc cagcgtccga tcgcccggaa gaactcgcgg
      121 gtcaccgttt ttccggattt tacctcgatc gcgtccagaa gaccgtcgtt ttcgatcagg
      181 aagtcgatct cgaagccgct tcggtcgcgc cagaaataga gttcgggttc gcgcccttcg
      241 ttgaggcgcg ccttcaaaag ctcgacggcg accatgttct cgaagacggc gcctcgcagg
      301 gggtgtgttt cgagctgctc ttcgttccgg ataccgagaa ggtggcagag cagcccggtg
      361 tcgtggaagt agagtttcgg agtctggagg aggcgcttat tgaagttgcg gaagtacgga
      421 cggacaagaa agacgatgga gcctgcctcc aggatggaaa gccacgcctt ggcggtgttg
      481 tggacgacgc cgcactcccc cgccagagaa gagaagttga cgagctgccc ggccctcccg
      541 gagcacaggc gaaggaagcg ctgaaaagtg gagaggtcgc ggacgttgag catcgagcgt
      601 acatcgcgct caaggtaggt cgcggtatag ttggcgaacc atgcggttct ctccactgtt
      661 cgggtgtgca gcggaggata gccgccggac aagatcgctt cgtcggcgct ttcggggagc
      721 aacccggcgc ttcgcagttc gtcgaaagag aagggaggga gctgcaggag cgccgttctg
      781 ccggcaaggc tctgtgtgat gctctccaga aggtgaaagt gctgcgagcc ggtgagaacg
      841 aagagcccca tcctcccgtc gaggtcgatc ctcccctgaa gataggaaaa gagcgacggg
      901 acgcgctgcg cttcgtcgaa gagagcgccg tcggggaagc gatccagaaa cgagcgggga
      961 tcgttctccg cgaattcgcg ctggtcggga tcctccagcg agacgtacgg tttctccgga
     1021 aagagatacc gggcgagcgt ggtctttccg gactgtctcg cgccggtcac ggcaagacag
     1081 gggaagcctt ccatcagttg taaggccgtt ttttccattt ttcgcgggat atacatccgg
     1141 catcacctcg ttcatgctcg ttcttcggcg gaaagaccct gtacaaagtg gagggtactc
     1201 gattcccggc tgattgtcaa ttcaattgcc aatcagccgg agtgcggtat tccgtggttg
     1261 acctgaaagt cgaccgtgac gaggtcgttc cgtttattct ccgaagcatc gaatccgcac
     1321 ggaaatatcc tcattatgat aaaattgcgc tatcctcatc aggaggtgat tttatgccgc
     1381 acattcgccc tgtttcggat ctgcgaaaca aattcgcgga gatttcgaag attgtccacg
     1441 agtgtcagga gccgattttt cttacgaaga acgggtacgg cgatatggtt gtgatgagta
     1501 tcgagcagtt tgaaaggctc cagtttgaaa acgaaatcgt cttcaagctg agagaggcgg
     1561 aaaaccagtc aagcgccgac gacaggcgat acagccacgc cgatgtgttt gatgcgcttc
     1621 gggaacgtct ggccgatggg cgaaaaatat aatatcaggt atctcgaact ggcgcgttcc
     1681 gatcttttgg gtattgccga atatgtaaat tcgcaggctt gggagagaga tgcggcgaac
     1741 cggctggtag actctttgga gaaggcgatc ttgcgtcttg aaagctttcc gtacagcgga
     1801 gcgctctacg gaaaatcttt tggtttcaaa gaagagcata gaatgcttgt cgtcgggaat
     1861 tacctcgtct tttatgtcgt gtacgatgat tttgtggaaa tacgcagggt tattcatggg
     1921 aagcggcgtt tcttcgacct tcttcagccg ctttcgtgac agcgcggcgc ggaggggtct
     1981 tcggaaagcc ccttccgcgc tccgtactcc gtctacggcc agttgatcgt cctggggacg
     2041 tcgtcgaagt cccacatcat gttcaccgcc tggcttatgg acttcagttc atcgtactgt
     2101 tcctcgttca gcaggtcttt cagcgcgccc gcaatctccg tccacccttt ccggacgttg
     2161 tcgtccacgg cgaggttgat caggggcgcc gtccgggcga agagcgctcc ggcctccagt
     2221 acggccgccg tgtcgtcgct gaaaattccc tttgcgctgt cgatacgcgc cttggagtag
     2281 aactggacgg tgaatacggg gatgtcgacg acggccttcc gcagcttggc gtcgtatgcg
     2341 gccagctcct gcttgatcga gaggatgccg tcgttgagaa cgcgcgtctt ctcgtagggg
     2401 accgagtgcc tggcgacgtc gatctcgaac tgattcaggt atttgacgta gtcggcggat
     2461 tgccgccgca atctggcgta gctcagcagc gcaaagttcc acgggctgtc cggcgcgttc
     2521 tccagaagcg ccgcgttccc ggctctaagg gtcttcagtt cggtaaggaa cgcctcttct
     2581 ttttcacttt ccgcgaacca ccccttgact atctcccacg tgtatttcag gccttcggtg
     2641 gttactggaa gcagctcctg cggggaagcg ccctttttca gcaggtcgaa gagcttgtcg
     2701 gcgggcgaag gttccgttga tttttccgaa agcgcggtgt acgccgagtt gatcgtgttc
     2761 cagatagtgt tgaaccggtc ggtgaattcg ccgagctttt cgtcctgtct gcgtgctccg
     2821 cgttcgcttt tcccgacgag ggagtagagc atggagtcgt acatatcgag gccgtaggag
     2881 aggagccaga ggatatccgc ctgcggcagg gagaggatgt ctttcggctc cgttccgccg
     2941 agcgttctta gcttttccag tccaagatgg tcaagatcgc cccggttttc tatgccgcga
     3001 agttcggcga gcctgttatc cagagtttcg gcggcgtttc ctgctgcgtt cgacaggagc
     3061 agcaggcaga gggagagaag cagcgttccg tacagttttt tcataacgca ttcctcctga
     3121 aggtgatttt tactcattga acctccggag cgggagaagc atctctttcc cgctccggtt
     3181 tcacgtggct tcagttgagc atgctcgacg tgaggtaagg gggcagcgcg cccccggcga
     3241 tcacgtggat gccgttttcc gcgaggtgtt ccagggctct ttcggtggct tccccgagac
     3301 ggacgatctg ctcggccagt tcggggtcgc tcttttccac tctctgcggg tcgaacttgt
     3361 agatgtggta gttgccttcc acaagcgggc tttggacgaa ctccagcaaa atcgcgagcg
     3421 tcttggaatc gttcattcgg gatacctcct ccgttctctt atcaaggtgc ttcagggcgt
     3481 cgttccgccc ggaccgaagc tgtgcgcgtg aagagtaacg acggacgttg cgggaaccga
     3541 acacacatca tgtccagtgt ttttgggtcg tgtgcatcgt tcagtgagat ctggaaattt
     3601 tcttgctccg cttcatcctc cgccgggaag gatggtccga aagccgtctg cctgagaggg
     3661 taatgagaga tatacaacaa atgggaatta atagtagcaa tagccgcgcc gttcgtcaag
     3721 agcccgaatg cgggaaacct cttgacgaac ggcgcaagaa aaaacgtcag gtcaacctgg
     3781 agcggttcgg ccgacagttc agcgagggtg ctcggccagt cggcgtacct gctcttccgg
     3841 aaggtcgacg agttcggcga cgagggaaag cgccatgccc ttatccagca tgcgccggac
     3901 cgtctggagc ttcgccagtt cgacgccttc tttgcgtcct tcttcccgac cttcctcccg
     3961 accttcttct cgcccctctt ctcgtccttc ttttttgagt ttccggattt ccttctccag
     4021 ataggtcagc acttcgtcc
//
LOCUS       DAOTIL010000150         4025 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminobacteriaceae bacterium isolate AMR_MDS_3026
            NODE_26465_length_4025_cov_2.962469, whole genome shotgun sequence.
ACCESSION   DAOTIL010000150 DAOTIL010000000
VERSION     DAOTIL010000150.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868050
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminobacteriaceae bacterium (wastewater metagenome)
  ORGANISM  Aminobacteriaceae bacterium
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae.
REFERENCE   1  (bases 1 to 4025)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4025)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:19:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,224
            CDSs (total)                      :: 3,198
            Genes (coding)                    :: 3,184
            CDSs (with protein)               :: 3,184
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4025
                     /organism="Aminobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_26465_length_4025_cov_2.962469"
                     /isolate="AMR_MDS_3026"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:3034609"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>4025)
                     /locus_tag="P5201_04310"
     CDS             complement(<1..>4025)
                     /locus_tag="P5201_04310"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRV97787.1"
                     /translation="LSHSGCAPKAFDLQVAPNMQEVSWSLAPLFDTVSLTVTVRDGDG
                     GKEIPGARVVLAEAWPRGEIAQKTTDASGSAAFAGLKIGRLNRAGADGRMTLSRSAVT
                     VRAEADGYEPMLVPVLLGRTASVEVQLNSAEKIAAQPGNLDFSSAQRIRTGAPVDFVI
                     ENAGENKFFRFHLPYPAWVGLDANRGQNPWLRFHLHAADGTEIGNWGLSGESALFDAG
                     ALPAGEYVLQAREWNNNKAATLPMTFTIQCRWASDPHSPNAELSSARAFRTGEEVRGY
                     ILPRGDRNHFRFEIKRPGQVRLWLQRPENIWIRAFLFRSDGAEIANLGCSAATAEAVY
                     RLDPGAYVVDIREWNNNAASLDPYTLRIEEIEDDGMDDAKQEEASVRIFRPLPLASLR
                     GGTIFPQGDRDWYQATLPSAGVFKAFLLRSPSVWQRITLFDTKGVRLTDAGVSGPRGD
                     LAWHADGPATVFLQVREWNDNAADPSPYILGTFFEPCDQLDAQGRNDSPDTASPLELN
                     EWTRGNLLPNGDNDWFRIDVDHPGILRLEGATPPGIWVRLALHGGNAEQLADVGYSRP
                     SSEFTWENPVLPGAYFIRLREWNNNASNPGIYSLRAVLQRSEPEESVPLAEDAVRTLT
                     PGEARPFFIDHGGDRDVFLFDIAQQGPFWLKIWKPGNIWIRARLYDDRTGAELKKIDL
                     SGASYNGKFEAEGPTRYRLELGEWNNNAYSVQPGFIQVRPDDAQITADEIKAVPDAVT
                     PTLVTFTRSAWAHMQIPRARTIEVDATGDGKTDFSFRPGDTATWRYPAEGLYKAAALM
                     QGEGGATTRLPFWVDVSGPRERTGIHLTVDVPGEGQRVEDEAPARARAMSYSGSPIAR
                     IEASVDGTQLPPAYTPPYTFEAPWKSLPRAAKEHLFTFKAVDGRGESATVERRISISE
                     YFGLRPADGAVLSGNQVRVSWSSGEFSPTKVRYRAKGTEEWTEIVGESGREHAVFLED
                     LESGTAYEFQPLGGSEEGPVRTLTRVKGLAFGKSRYGASIERTYDQRFPISVRNHAEE
                     AMKVVLTSGKLEEEGLFIGFVGEGSEGEPFDLAPGEEREFLLVLNAQNAVKPKHVFPV
                     KISSANGFADEAEVELEVRLPVVKLEWEDLGEAPKGPGRRLRLVNRGDSLTDLSVVTD
                     TGDLKISPAINHGFLPSGQSVEIAVTPRLYEGFREARGAVTAKSIATESSFPVHLALP
                     EGKEIFGVNLMPKMPEETADEDERVLLEARALSAAFLTPDYIDASMWGNGIDSNGDGR
                     IDRWSFIDQEERILWIGEDASGDGEIDFVRADVGFDGRFDYSALKSAKGWEETNLLDA
                     WLEMTFTLPWNRGTYEPHDLD"
BASE COUNT          636 a         1366 c         1288 g          735 t
ORIGIN      
        1 tgtcgaggtc gtgcggctcg taggttccgc ggttccatgg aagggtgaag gtcatttcga
       61 gccaagcgtc cagcaggttg gtctcctccc acccctttgc gctcttgagc gccgagtagt
      121 cgaagcggcc gtcgaagccg acgtcggcgc ggacgaagtc gatctccccg tccccgctcg
      181 cgtcctctcc gatccagagg atgcgctcct cctggtcgat aaagctccat cggtcgatcc
      241 tcccgtcgcc gttggaatca atcccgttcc cccacatgga ggcgtcgatg tagtcgggcg
      301 tcaggaaggc cgcggagagc gccctggcct caaggagcac acgctcatct tcatccgccg
      361 tctcctccgg catcttcggc atcaggttga cgccgaagat ctccttccct tcgggaagcg
      421 ccaggtggac agggaacgac gactccgtgg cgatggactt cgccgtcacc gcgccccgcg
      481 cctcgcggaa tccctcgtag agcctcggcg tcacggcgat ctccaccgat tggccggagg
      541 ggaggaagcc gtggttgatc gccggggaga ttttcagatc gcccgtatcc gtaacgaccg
      601 acaggtcggt gagactgtcc cccctgttca ccaggcgcag acggcgtccc ggccccttcg
      661 gcgcttcgcc gagatcctcc cactccagct tcacgacggg gaggcgcacc tccagctcca
      721 cctccgcctc gtcggcgaaa ccgttcgcgg agctgatctt gacggggaag acgtgctttg
      781 gcttcacggc attctgcgcg ttgaggacga gcaggaactc ccgctcctcg cccggcgcca
      841 gatcgaacgg ctccccctcc gacccctcgc cgacgaagcc gatgaagagc ccctcctcct
      901 ctagcttgcc ggaggtgagc acaactttca tcgcctcctc ggcgtggttt ctgaccgaaa
      961 tggggaaccg ctggtcgtag gtgcgctcga tggacgcgcc gtagcggctt ttgccgaagg
     1021 cgagcccctt gacccgcgtg agggtccgca ccggcccctc ctcgctccct ccgagcggct
     1081 ggaactcgta cgccgttccg gattccagat cctcaaggaa tacggcgtgt tcgcggccgc
     1141 tctcgcccac gatctccgtc cactcttccg tccccttcgc cctgtagcgg accttcgtcg
     1201 gcgagaactc cccgctcgac cagctcacgc ggacctggtt gccggaaagc acggccccgt
     1261 ccgccggacg caggccgaag tactcggaaa ttgagatccg gcgctcgacg gttgcggatt
     1321 cgccccgccc gtcgacggct ttgaacgtga agagatgctc cttcgccgcg cggggaaggc
     1381 tcttccacgg cgcctcgaac gtataggggg gcgtgtaggc gggcgggagc tgcgttccgt
     1441 cgaccgaggc ctcgatgcgc gcaatgggcg agccgctgta gctcatggcc ctggcgcgcg
     1501 ccggagcctc atcctccacg cgctgcccct ctcccggaac gtcgaccgtc aggtgaatcc
     1561 ccgtccgctc ccgtgggccg gagacgtcca cccagaaggg aagccgcgtc gtcgcgccgc
     1621 cctcgccttg catcagcgcg gcggccttgt acagcccctc cgcggggtag cgccacgtgg
     1681 ccgtgtcgcc cggacggaat gaaaagtcgg ttttcccgtc gcccgtcgcg tcgacttcta
     1741 ttgtgcgtgc gcgggggatc tgcatgtgcg cccacgcgct ccgggtgaag gtcacgagcg
     1801 tcggcgtcac ggcgtcggga accgccttga tctcgtcggc agttatctgc gcgtcgtccg
     1861 gacgcacctg aatgaagccg ggctgaacgg agtacgcgtt gttgttccac tcccccagtt
     1921 caagccggta gcgcgtcggt ccctccgctt cgaactttcc gttgtaggaa gcgccggaga
     1981 gatctatctt ctttagctcc gctcccgtcc tgtcgtcgta gaggcgcgcg cggatccaga
     2041 tgttcccagg cttccatatc ttcagccaga acggcccctg ctgtgcgatg tcgaagagaa
     2101 agacgtccct gtcgcctccg tgatcgatga aaaacgggcg cgcttctccc ggcgtgagcg
     2161 tgcggaccgc gtcctcggcc agcggtacgc tctcttccgg ctcggaccgc tggagcaccg
     2221 cacggagaga gtagatgccg gggttggagg cgttgttgtt ccactcccgg aggcgaatga
     2281 aataagctcc ggggagaacg gggttctccc atgtgaattc agaggagggg cgtgaatatc
     2341 ctacgtcggc gagctgctcg gcgttcccgc cgtggagagc gagccgcacc cagatacccg
     2401 gaggggtcgc cccctccagg cggaggatac ccggatggtc gacgtcgatg cggaaccagt
     2461 cgttgtcgcc gttcgggagc aggttgccgc gcgtccactc gttcagttcg agcggagagg
     2521 ccgtatccgg cgagtcgttc cgcccctggg cgtcgagctg atcgcacggc tcgaagaacg
     2581 tccccaggat gtagggcgac gggtcggcgg cgttgtcgtt ccactccctt acctggagga
     2641 agaccgtcgc aggtccgtca gcgtgccagg caagatcacc gcgaggtccg ctcactccgg
     2701 cgtcggtcag ccgcacccct ttcgtgtcga agagcgtgat gcgctgccat acagaagggg
     2761 aacggagcag gaacgcctta aagacgcccg cgctcgggag cgtcgcctga taccagtccc
     2821 tgtctccctg ggggaagatc gtcccgccgc gcagcgaggc gagcggaagg ggcctgaaga
     2881 tacggaccga ggcctcctcc tgcttcgcgt cgtccatgcc gtcgtcttcg atctcctcga
     2941 tgcggagcgt gtagggatca aggctggcgg cgttgttgtt ccattcacga atgtcgacga
     3001 cgtacgcgcc ggggtcgagc ctgtagacgg cctccgccgt cgccgccgaa cagccgaggt
     3061 tcgctatctc ggcgccgtcg gaccggaaca gaaaggcgcg tatccagata ttctccggcc
     3121 gttggagcca gaggcgtacc tggccgggcc gcttgatctc gaaccggaag tgattcctgt
     3181 ccccgcgcgg gaggatatac cctcgaacct cctcgcccgt ccggaaagcc cgtgccgagg
     3241 agagctccgc gttcggagaa tgcgggtcgg aggcccagcg gcactggatc gtaaaggtca
     3301 tgggcaacgt cgccgccttg ttgttgttcc actcccgggc ctggagcacg tactcgcctg
     3361 ccggaagcgc cccggcgtcg aagagcgcgc tctctccgga gagcccccag ttgccgatct
     3421 ccgttccgtc cgccgcgtgc agatggaagc gtaaccacgg attctgtcct ctgttcgcgt
     3481 ccagtccgac ccatgcgggg tacggcagat ggaagcggaa gaacttgttc tctcccgcgt
     3541 tttcgatgac aaaatctact ggagcgccgg tcctgatccg ctgcgccgag gaaaaatcga
     3601 gattgccggg ctgtgccgcg atcttctccg ccgagttgag ctgcacttcc acggacgccg
     3661 tccgaccgag aaggacgggg acaagcatcg gctcgtaccc gtcggcttcg gcgcgcactg
     3721 tgacggcgct cctgctgagc gtcatcctgc cgtcggcgcc cgcgcggttg agccgcccga
     3781 tcttgaggcc ggcaaaggcc gccgatccgc tcgcgtccgt cgtcttctgg gcgatctctc
     3841 cccggggcca cgcctcggcg agaacgacgc gcgcgcccgg gatctcctta cctccgtcgc
     3901 cgtccctgac ggtcacggtg agcgagaccg tatcgaagag cggcgcaagc gaccacgaga
     3961 cctcctgcat attcggcgcg acctggaggt cgaaggcctt cggcgcgcat ccggaatggc
     4021 tgagc
//
LOCUS       DAOTIL010000151         4021 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminobacteriaceae bacterium isolate AMR_MDS_3026
            NODE_26498_length_4021_cov_3.085981, whole genome shotgun sequence.
ACCESSION   DAOTIL010000151 DAOTIL010000000
VERSION     DAOTIL010000151.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868050
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminobacteriaceae bacterium (wastewater metagenome)
  ORGANISM  Aminobacteriaceae bacterium
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae.
REFERENCE   1  (bases 1 to 4021)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4021)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:19:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,224
            CDSs (total)                      :: 3,198
            Genes (coding)                    :: 3,184
            CDSs (with protein)               :: 3,184
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4021
                     /organism="Aminobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_26498_length_4021_cov_3.085981"
                     /isolate="AMR_MDS_3026"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:3034609"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            80..262
                     /gene="secE"
                     /locus_tag="P5201_04315"
     CDS             80..262
                     /gene="secE"
                     /locus_tag="P5201_04315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013047723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecE"
                     /protein_id="HRV97788.1"
                     /translation="MGNILTFVREARAELKKVTWPGKKQVWYSTIVVIAFTLLVSAYL
                     GIVDMVLTGVFSRLFS"
     gene            350..892
                     /gene="nusG"
                     /locus_tag="P5201_04320"
     CDS             350..892
                     /gene="nusG"
                     /locus_tag="P5201_04320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005659220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination
                     protein NusG"
                     /protein_id="HRV97789.1"
                     /translation="MDSIEARRWYIVQTYAGYENRVKANLEQRIATMGMEDSIFGVLV
                     PVEERVSIKEGKSKKVTRKLFPSYVLVEMRLNDQSWYVVRHTPGVTGFVGSGNHPIPL
                     TEKEVKEIMGKVGKDQAKPKIEINFRPGDVVKVKSGPFEGHVGPVVEIFPEKGKVKFS
                     VTVFGRETSVETDYTELEKL"
     gene            1001..1426
                     /gene="rplK"
                     /locus_tag="P5201_04325"
     CDS             1001..1426
                     /gene="rplK"
                     /locus_tag="P5201_04325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006582925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11"
                     /protein_id="HRV97790.1"
                     /translation="MAKKVIAEIKLQLPAGKATPAPPVGPALGQHGVNIMEFVKQFNA
                     KTADQAGLVIPAVITVYADRSFTFVLKTPPASVLLKKAAGLESGSGEPNKKKVATLDR
                     AKVREIAELKKVDLNSYDVDAAMRMVEGTARSMGITIVD"
     gene            1489..2199
                     /gene="rplA"
                     /locus_tag="P5201_04330"
     CDS             1489..2199
                     /gene="rplA"
                     /locus_tag="P5201_04330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006301575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L1"
                     /protein_id="HRV97791.1"
                     /translation="MAKLSKRLTAAHQKIEDGKQYTVKEAIALFKETATAKFDESMEV
                     HIRLGVDPRHADQQVRSTVVLPHGTGKTKRVLVLAMGEKVKEAEEAGADFVGGEELVG
                     KISGGWLDFDAVIATPDMMKSVGRLGKVLGPRGLMPSAKTGSVTFDIASAIKEIKAGR
                     VEFRVDKFAIVHNAIGKKSFSNEQLFDNAKTLIQAVLKARPASVKGTYVKSLNLSSSM
                     GPGIKIDVASAQKEVAGE"
     gene            2387..2920
                     /gene="rplJ"
                     /locus_tag="P5201_04335"
     CDS             2387..2920
                     /gene="rplJ"
                     /locus_tag="P5201_04335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013047727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L10"
                     /protein_id="HRV97792.1"
                     /translation="MPATLKYEQVDELKGRLARTQAIFACEYRGMTVAQITALRAQVR
                     AAGGEMKVAKNTLMRIAMEQEGFIPFPDDMAFGPNVFALAYGDPVAVAKALRDFAKDR
                     TNKFFVLKGCVLGQTLLDATQIGALADLPSKEVLIGQVVRTIAAPIAGLLTVLSGPAR
                     GLVTCLSQIKEKKEDVA"
     gene            2995..3375
                     /gene="rplL"
                     /locus_tag="P5201_04340"
     CDS             2995..3375
                     /gene="rplL"
                     /locus_tag="P5201_04340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012870240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L7/L12"
                     /protein_id="HRV97793.1"
                     /translation="MTRDEMIQAIENMTVLELSELVKALEEKFGVSASAPAMAMPMMA
                     MGAAPAAAAEEEKTEFDVIYKAPGANKINVIKVVRELTGLGLKEAKELVDNPPKAVKE
                     AVSKEEAEEIRKKLAEAGADVEVK"
     gene            3447..>4021
                     /locus_tag="P5201_04345"
     CDS             3447..>4021
                     /locus_tag="P5201_04345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014806118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HRV97794.1"
                     /translation="MIDCHVHVYPPEIIRDAAVIGKNEPYFELLSTGKVHKWATAEDV
                     IAAMDRDGIDVSWIFGFAFKDPGLCALCNEYVIEAVRKYPTRLKGLAVVPPCAPGAGN
                     EIARCREQGLVGVGEIFPGGQDFDISDVRQTWRLAGAVAEAGMFLLVHSAEPVGHDYP
                     GKGNTGPREAAEFCLHHPEVEVVFAHFGGGL"
BASE COUNT          981 a          983 c         1201 g          856 t
ORIGIN      
        1 aaaaccgggg gttgcaggtt cgagtcctgc cgggcctgcc atttttttat tgtcaaaaac
       61 aagatggggg gcagaggttg tgggaaatat cctcaccttc gttcgggaag cccgggccga
      121 gttgaagaaa gtaacgtggc cggggaaaaa gcaagtctgg tattcaacaa tagtcgttat
      181 cgcttttacg cttttggttt cggcctatct cgggatagtt gatatggtac ttacaggagt
      241 cttttccagg ctctttagtt aaaagcccgc tgccggtcca ggcagttgat accgggatca
      301 tttcagagat cggcagggag gtgagggggc cgcaagaccc ccttaaagta tggacagtat
      361 tgaagcgcgc cgctggtata tagtacaaac gtatgcaggg tacgaaaatc gcgtcaaggc
      421 aaatctcgaa cagagaattg ccacgatggg tatggaggac agtattttcg gcgtcctcgt
      481 ccctgttgag gagcgtgtct ccataaaaga gggaaagagc aaaaaggtga ctcgcaagct
      541 ttttcccagt tatgttcttg tcgagatgcg tcttaacgac cagtcgtggt atgtcgttcg
      601 gcataccccc ggcgtgactg gctttgtcgg ctccggaaac catccgattc ctctcactga
      661 aaaagaagtg aaagagatca tggggaaggt cggtaaagac caggcgaaac ccaaaatcga
      721 gattaatttc cggcctggcg atgtcgtcaa ggtaaaatcc ggaccctttg aaggtcatgt
      781 ggggccggtt gtcgagattt tcccggaaaa gggaaaagtg aagttctctg tgacggtctt
      841 cggccgggag acatctgtag aaactgacta tacggagctt gagaagctct agatatatat
      901 cagcgcgaag gcccgcattc cagggaggca agagaactct tggctcctgt ttttccctgt
      961 gtgcggaagt gctttcagta acgagaggag actttgagga atggcgaaga aagtaatagc
     1021 agagatcaag ttgcagttgc ccgcaggcaa ggctacgcct gcgccccccg tgggacctgc
     1081 tcttggtcag cacggagtga atatcatgga gttcgtgaag caattcaacg cgaaaacagc
     1141 agatcaggcc gggcttgtaa ttcccgccgt gatcacggtg tacgcggaca gaagcttcac
     1201 cttcgtgctc aaaacaccgc ccgcgagcgt gctgctcaag aaggcggccg gattggaatc
     1261 cggctccggc gaaccgaaca agaagaaggt agccaccctt gacagggcga aagtgcgcga
     1321 gattgcggag ctgaaaaagg tcgatctgaa ttcctatgac gtcgacgccg caatgcgtat
     1381 ggtcgaggga actgcgcgtt caatgggtat cactatcgtc gattgatgaa aagtggtttt
     1441 gaatttgtgg gaggcctcaa ggctgtctga ccaccaggag ggaaaaaaat ggcgaagttg
     1501 agtaaaagac tgactgcagc acaccagaag atcgaggacg gaaaacagta caccgtgaaa
     1561 gaggcgatcg cgctttttaa agagacggcg accgcgaagt tcgacgagag tatggaggtc
     1621 catatccgtc tcggcgtaga tcctcgtcac gctgaccagc aggtccgcag cacggttgtg
     1681 ctcccccatg ggacgggaaa gaccaagaga gttctcgttc ttgccatggg tgagaaagta
     1741 aaagaagcgg aagaggccgg agctgatttt gtcggcggcg aagagcttgt gggcaaaatt
     1801 tccggcggat ggcttgattt cgacgccgtc atcgcgaccc ccgacatgat gaaatcggtc
     1861 ggacgcctcg gaaaggtgct tggtccccgc ggccttatgc ccagcgcaaa gaccggcagc
     1921 gtgacgttcg atattgcgtc cgcgataaaa gagatcaagg ccggacgcgt cgaattccgc
     1981 gttgacaagt tcgctatcgt gcacaatgcg atcggcaaga agagcttctc caacgagcag
     2041 cttttcgaca atgcgaagac gcttattcag gctgtcctga aagcgaggcc cgcctcggtg
     2101 aagggaacct acgtgaagag ccttaatctt tcctcttcca tgggccccgg aatcaagatc
     2161 gacgttgcgt ccgcgcagaa agaggttgca ggcgagtaac gcgcttcctt tcctctgctc
     2221 aaaacaaaat atgaacttga actgaagaca gcgggtccct taaggggaca aaggccaggc
     2281 cgcccgccga ggttccggtg agtcgatcgg catctgctgc cgtacaggta ctcaatcccg
     2341 ggcccggcgc ccgggatttt ttactttcac cgaaggaggt gagtacatgc cggcaacact
     2401 taagtacgaa caggtagatg aactgaaggg gcgtcttgcc cgcacacagg ctattttcgc
     2461 atgcgaatac cgcggaatga ctgttgcgca gattacggcg cttcgcgccc aggtgcgcgc
     2521 tgcaggcgga gaaatgaagg ttgcgaagaa tacgcttatg cgtattgcca tggagcagga
     2581 ggggtttatt ccattccccg acgatatggc gttcggaccg aacgtcttcg cgctggcgta
     2641 cggggatcct gtcgcggtag cgaaagctct tcgtgatttc gcgaaggaca gaaccaacaa
     2701 gttttttgtc ctgaaggggt gcgttcttgg acaaaccctt cttgatgcga cacagattgg
     2761 cgctctcgcc gatctcccgt ccaaggaagt ccttatcggt caggtcgtcc gtaccatcgc
     2821 ggccccgatt gcgggtctgc ttacggttct ttccggtcct gcaagaggac tggtcacctg
     2881 cctcagccag atcaaggaaa agaaggaaga cgtcgcatag tttttgcgcc ggaagcacat
     2941 cgcatacacg gtatgcgata cacaagatga tatatatcca aggaggaaaa aagaatgacc
     3001 cgcgatgaaa tgattcaggc tatagagaac atgacagtgc ttgagctctc cgagctcgtg
     3061 aaggctcttg aggagaagtt cggagtttcc gcttccgctc ccgcgatggc gatgccgatg
     3121 atggcgatgg gcgcagcccc cgccgctgcc gccgaggaag agaagaccga gttcgacgta
     3181 atctacaagg cgccgggcgc caacaagatc aacgtgatca aggttgtccg cgaactcaca
     3241 ggccttgggc tcaaagaagc caaggaactt gtcgacaacc cccccaaggc agtcaaagaa
     3301 gcagtcagca aggaagaggc agaggagatc aggaagaagc tcgccgaggc cggagccgac
     3361 gtcgaggtta agtagttcgc ctttcctttt ttctggtata ataaagacgg tgcgaaagca
     3421 tcgtcttttt ttatgggggg tatctcatga tcgactgtca tgtacacgtc tatccgcccg
     3481 agatcatcag ggatgcggca gttataggga aaaatgaacc gtatttcgaa ctcctctcga
     3541 cgggcaaggt acataaatgg gccacggcgg aggacgttat cgccgcgatg gacagggacg
     3601 gcatcgacgt ctcgtggatt ttcggtttcg ccttcaaaga tcccggactc tgtgcgctct
     3661 gcaacgagta tgtgatcgaa gctgtacgaa agtacccgac acgcctgaag gggcttgccg
     3721 tggttcctcc gtgcgctccg ggcgcaggga acgaaatcgc ccgatgcaga gaacaaggtc
     3781 ttgtaggcgt cggagaaata tttcccgggg gccaggattt cgatatctcc gatgtccgcc
     3841 agacgtggag gcttgccgga gcagttgccg aagcggggat gtttttgctt gttcattccg
     3901 ccgagcccgt cggccatgat taccccggaa agggaaacac cggtccgcgg gaagccgcag
     3961 agttttgttt gcatcatccc gaggtcgaag tggtttttgc tcatttcgga ggggggctct
     4021 g
//
LOCUS       DAOTIL010000152         3999 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminobacteriaceae bacterium isolate AMR_MDS_3026
            NODE_26675_length_3999_cov_4.543864, whole genome shotgun sequence.
ACCESSION   DAOTIL010000152 DAOTIL010000000
VERSION     DAOTIL010000152.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868050
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminobacteriaceae bacterium (wastewater metagenome)
  ORGANISM  Aminobacteriaceae bacterium
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae.
REFERENCE   1  (bases 1 to 3999)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3999)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:19:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,224
            CDSs (total)                      :: 3,198
            Genes (coding)                    :: 3,184
            CDSs (with protein)               :: 3,184
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3999
                     /organism="Aminobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_26675_length_3999_cov_4.543864"
                     /isolate="AMR_MDS_3026"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:3034609"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1677)
                     /locus_tag="P5201_04350"
     CDS             complement(<1..1677)
                     /locus_tag="P5201_04350"
                     /inference="COORDINATES: protein motif:HMM:NF015418.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HRV97795.1"
                     /translation="MALVKENMHITLKQAWTLLREAITQIAPELCSVQHCYERLLAAD
                     LLSQRNVPHYNASAMDGFALSSASTAHATPSSPVFLRRDEFARLNTGGVLPASFDAVA
                     MIEDVALSSDGEKLTVAKTLVAGENVRPVGEDVFRGQVIARVGERISPPLASLCATAG
                     IPSVSVQALPKTLFIPTGDEIIPLEEWLSMDEPPAGKVGESNSFLVQGYFSRWGFPVD
                     IAPCLPDNPEVLSEFLLKKAEEYDLLLVGAGSAKGEKDYTFSIFEKYGKPIFRWLLMK
                     PGRPASAALLRRAVAVNLPGFPMSNAVILWSIVYPILQLLQKGDFDESSAIQEALGAE
                     GSEEMSLLVSCSSSPGKEEWLRLKCVEIDGRKMAYPLPSGASALWSLAEIDAVSLLPL
                     ESAELPKGSAVSVWTTRRIPWKERILFQGSNDPALERLPSFVRKQGGDLLLRSVGSLA
                     GLAALSRRECHIAAAHLFHAETGEYNTPFIDELFHDPETVVRRRVFFREQGFITKKGN
                     PKKISGVRDLDREDIVLVNRQRGAGTRVLLDALLEEADIHPSAVRGYNSLS"
     gene            complement(1665..2864)
                     /locus_tag="P5201_04355"
     CDS             complement(1665..2864)
                     /locus_tag="P5201_04355"
                     /inference="COORDINATES: protein motif:HMM:NF015418.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030151 - molybdenum ion binding [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin molybdotransferase MoeA"
                     /protein_id="HRV97796.1"
                     /translation="MSAIEYAAASLSFPWQGNESSRDLDAAEGLTLARDILAPEDFPP
                     FDRSTRDGYALISEDSYGASQSNPAFLTIRGEVPMAAVPSFSLSEGECALIHTGGVLP
                     AGADAVIMLENVERTGNWLEIRKAVQKGENIVFRGEEFSAGAILLPKGVQVDFKSSGV
                     LAAAGISKAPVQHVKVGILSTGDEIVTCETRSLNPGQFRDANANILSSLLRRHGYEPV
                     YLGIVEDSKVSLEKALYRAMEECDVVLLSGGSSVSTRDHCSTILESLEAPGLLVRGIL
                     MSPGKPTLIAGIKEKQKLVMGLPGHPFSCFLAAYTVFLPLAHAMNTGAIVSPWRSVVL
                     PVSESVFGHSGIEEFLPCALRDGKAVPLPVKSSFAKALGQADGILRLSSSRETVRPGE
                     EVEVWLW"
     gene            complement(2983..3624)
                     /locus_tag="P5201_04360"
     CDS             complement(2983..3624)
                     /locus_tag="P5201_04360"
                     /inference="COORDINATES: protein motif:HMM:NF016895.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SPOR domain-containing protein"
                     /protein_id="HRV97797.1"
                     /translation="MSIRESRRHKERRSVIPFGDIMLPVIGLVAVGLLIVGVKLIFLS
                     GDRYKTYTPLPIHQEEKTTPNPSVTPPVLPPGQPEKENAAEDAILAVPVSPGGGTGDR
                     VRVETPTKTPPVVKPKTPPRPPEVKRPAPPPAPSNSRWGVQIGSFTARQSAETVRQQA
                     ERAGFRVSILSGQVRGKTYFRVTVPAGNTRAEAVSLSQKLAKSGFPVFVVSIR"
BASE COUNT          988 a         1062 c         1006 g          943 t
ORIGIN      
        1 actcagacta ttgtatcccc tgaccgcgga aggatgaatg tctgcctcct ccagaagcgc
       61 atcaagcagt acacgcgttc ccgcgcctcg ctgtcggttg acgagcacaa tatcctcccg
      121 gtcaagatct cgtaccccgg agatcttctt ggggttgcct tttttcgtaa taaaaccctg
      181 ttcccgaaag aaaactctcc tccggacaac cgtttccgga tcatgaaaaa gttcgtcgat
      241 gaacggagtg ttgtactctc ccgtctcggc atggaataga tgcgccgccg cgatatggca
      301 ttctcttctc gaaagagcgg caagtcccgc aaggcttccg accgatctga gcaggagatc
      361 gccgccctgt ttgcggacaa atgaggggag gcgttccaag gcaggatcat ttgacccttg
      421 gaaaagaatg cgttccttcc agggaatacg ccgggtcgtc cacaccgaaa cggcgcttcc
      481 cttcggcaat tccgcgcttt ccaacggaag cagcgacact gcgtcaatct ccgccaagga
      541 ccagagtgcg cttgcccctg aaggcagcgg atacgccatt tttcgcccgt ctatctccac
      601 gcattttaaa cgaagccatt cctcttttcc cggcgaggaa gagcaggaga cgagaagaga
      661 catctcttcg gatccttccg ctccgagagc ttcttggatt gcggaagatt cgtcaaaatc
      721 ccctttctga agaagctgta aaatcgggta gacgatcgac cagaggatga cagcgttcga
      781 cattggaaaa cccgggagat tgaccgcgac tgcacgtcgt agcagcgcag cggatgcagg
      841 tctgcctggt ttcatcagaa gccatcgaaa gattggcttc ccgtattttt caaagatgga
      901 aaaagtatag tccttttctc cttttgccga gccggcccca acgagcagca ggtcgtactc
      961 ctcggctttt ttcaaaagaa actccgaaag tacctctgga ttgtcgggaa ggcatggagc
     1021 tatatcgaca gggaaacccc atcgggaaaa gtagccctga acgagaaatg agttgctctc
     1081 ccctactttc ccagcaggcg gctcgtccat ggaaagccat tcctctagag ggatgatttc
     1141 atctcctgta gggatgaaga gagtcttcgg aagagcctgt acgctgactg aaggaattcc
     1201 cgccgtggcg cacaaggatg ccagcggcgg cgatattctc tcccctacac gcgctattac
     1261 ctgtcctcga aagacatctt caccaacagg acgaacattt tctccggcaa cgagcgtttt
     1321 ggcgacggtc agcttttctc cgtcactgga gagcgccacg tcctcaatca tagcaaccgc
     1381 atcgaaggat gcaggcagca caccgcccgt attgagccga gcaaactcgt cgcggcgtaa
     1441 aaatacaggc gaagatggcg tcgcgtgggc agtggaggct gatgagagtg cgaatccgtc
     1501 catggcggat gcattgtagt gcggaacgtt tctttgtgag agaagatccg cggcaagtaa
     1561 tcgttcataa caatgttgta cagaacataa ctcgggggcg atctgtgtga ttgcctcccg
     1621 aagaagagtc cacgcctgtt tcagtgtaat gtgcatattt tcctttacca aagccatact
     1681 tctacctcct cgccgggacg gacagtctct ctcgaagacg aaagacggag aatcccgtcg
     1741 gcttgtccga gcgcctttgc gaacgaagat ttcaccggaa gaggaacagc ctttccgtcg
     1801 cggagggcgc atggtaaaaa ttcctcaatc cccgagtgac cgaatacgga ctcggaaaca
     1861 ggaagtacta ccgatctcca aggggatacg atggcgccag tattcatagc gtgtgcaaga
     1921 ggaaggaaca cagtatacgc agcaagaaaa catgaaaaag ggtgtcccgg caggcccatt
     1981 acgagctttt gcttttcttt tattccggcg atgagagtgg gtttccccgg cgacatcaaa
     2041 atgcctcgaa ccagcagtcc cggtgcttcg agggattcaa gaatcgttga acaatggtct
     2101 ctagtgctca ctgaagaccc cccgctgagc aggacaacgt cgcactcctc catggctcgg
     2161 tataacgctt tttcaagaga aacctttgag tcttcgacta ttcccaaata gacaggttca
     2221 tatccatgac gccgtaaaag cgacgaaaga atgtttgcgt tggcgtcgcg aaactggcct
     2281 gggtttaaag aacgagtttc acaggtgact atttcgtctc ccgtgctgag aatacctacc
     2341 tttacgtgtt gcaccggcgc ctttgaaata cctgcggctg caagaacgcc ggaagacttg
     2401 aagtcaacct ggacgccctt tgggagaagg atggcgcctg ctgagaattc ttcaccccga
     2461 aagacgatat tctcgccctt ctgaacagcc tttcgtattt ccagccaatt tcctgttcgc
     2521 tctacgtttt ctagcataat aaccgcatcc gctcccgcag gcagtacgcc tccagtgtga
     2581 atcagcgcac attccccttc gctcaaggaa aacgaaggaa cggcggccat tggaacttct
     2641 ccgcggatcg ttaaaaatgc gggatttgat tgcgatgctc cgtagctgtc ctcactgatg
     2701 agcgcatacc cgtcgcgggt gcttctgtca aaaggaggga aatcttccgg tgcaagaata
     2761 tctcgagcaa gagtcaaacc ctccgctgca tcgagatctc ttgaactctc atttccttgc
     2821 caaggaaatg agagtgatgc ggcggcgtac tcaatggcgg acattctgtc agtcagttcc
     2881 cgaacaaaac cagccaatag cgtatctcct tcccgatccg tgtgctctcc aaaatttgtt
     2941 gaagcctaca tgaagtggat aattacaaag caaaaaagcg ctttatcgaa tcgaaaccac
     3001 aaaaacagga aaaccagatt ttgcaagctt ctgcgagaga gataccgctt cggcacgagt
     3061 atttccagcc ggaacggtca ccctgaaata tgtcttcccg cgtacctgcc cggacaaaat
     3121 cgagactctg aagcccgctc tttcagcctg ttgacgtacc gtctcggcgc tctggcgtgc
     3181 tgtaaatgaa ccgatttgaa cgccccaacg actgttggaa ggagctggcg gaggcgcagg
     3241 tcgtttgact tccggtgggc gcgggggtgt cttaggtttt actactggag gagttttcgt
     3301 aggagtttct accctgacac ggtctcctgt gccacctcca ggtgaaacgg gaactgcgag
     3361 gatggcgtcc tccgcagcgt tttccttctc gggttgtccg ggcgggagaa caggaggtgt
     3421 gacggaagga ttgggcgttg ttttttcctc ctgatgaata ggtaacggtg tatacgtttt
     3481 gtacctgtcg ccggaaagaa aaatgagttt tacacccaca atcaggaggc cgaccgccac
     3541 aagtccgatg acgggaagca taatatcgcc gaaagggata acagatcgcc tttctttgtg
     3601 tcttctgctt tctcttatgg acatttttac cctccctgtc cgtctgcctc gcttggagaa
     3661 gaagtgcccc aaatggcttc ctaaatcagc aaaaatttgt cttctgcatt aaccccagtt
     3721 tgtagcgcaa cgtatttact ataatagtgc acacaagcct atctaataga tcagaatcct
     3781 aaaaaataaa gcaggaatcc ctttcaagac ccctgcttcc ttcagcctgc gctggacgat
     3841 tccggatcaa tcaaattcaa ctcgctcgag gaaatgattt tcgggatatc agaatttact
     3901 tgccggcttt tttatttctt cgaattatcg aaggaatatc gtaagggttc ttgggcacac
     3961 cactgatgga aaacatatcc tcatcctgta tcggcagta
//
LOCUS       DAOTIL010000153         3996 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminobacteriaceae bacterium isolate AMR_MDS_3026
            NODE_26704_length_3996_cov_3.630297, whole genome shotgun sequence.
ACCESSION   DAOTIL010000153 DAOTIL010000000
VERSION     DAOTIL010000153.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868050
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminobacteriaceae bacterium (wastewater metagenome)
  ORGANISM  Aminobacteriaceae bacterium
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae.
REFERENCE   1  (bases 1 to 3996)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3996)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:19:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,224
            CDSs (total)                      :: 3,198
            Genes (coding)                    :: 3,184
            CDSs (with protein)               :: 3,184
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3996
                     /organism="Aminobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_26704_length_3996_cov_3.630297"
                     /isolate="AMR_MDS_3026"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:3034609"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..792
                     /locus_tag="P5201_04365"
     CDS             <1..792
                     /locus_tag="P5201_04365"
                     /inference="COORDINATES: protein motif:HMM:NF014799.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase C-terminal domain-containing
                     protein"
                     /protein_id="HRV97798.1"
                     /translation="AGGLRPVVFIYSTFLQRAMDQLVHDIAMQNLPVTIAVDRSGLIG
                     DDGETHQGLLDISWSRSVPHLVVAAPRDEIGLSALFDLGLRRNGPMMIRFPRGEAIPS
                     LHRIRGEVFLDDLPVPEILEKGTECALLGYGKTVELMLDAREIIVRDGGVPPSVVDIK
                     CVKPMCVPVMEGLLKSHELVIVAEDGYKECGVGEAIASLAEKLRPSSRTAMVLPMGIP
                     DLFVPQGTVSEQAEYCGLTPRKVVTMYEEYQKRTHRQGSGIQGVR"
     gene            734..1561
                     /locus_tag="P5201_04370"
     CDS             734..1561
                     /locus_tag="P5201_04370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013048247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TlyA family RNA methyltransferase"
                     /protein_id="HRV97799.1"
                     /translation="MKSIKKERIDKVLVSRGFVETRTKAQSLLMAGKVTVDGNRVDKP
                     GALVFPDAAVEVIGTGNEWVSRGALKLLKALDVFPFDPKDRICVDIGASTGGFTQVLL
                     SRGALRVYSVDVGYGQLAWSLRSDERVVVMERTNARNLLPDNFPDIPDRAVADASFIS
                     LKLLLPAMDTILSPQGEAVVLVKPQFEVGKGKVGKGGVVRSSEDHIVVLEEILNFCLR
                     ETTFTPCGITFSPITGPMGNIEYLLYLSKSDSEPSIPHPVSIVEEAHSFFTKGRAAS"
     gene            1558..2439
                     /locus_tag="P5201_04375"
     CDS             1558..2439
                     /locus_tag="P5201_04375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015555795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(+)/NADH kinase"
                     /protein_id="HRV97800.1"
                     /translation="MNTPNIGMLVNTRKPGAIKMARMLLQWGMDKGIAFLFPPHEASV
                     LGVQGVSDEEWKETVGIALVIGGDGTFLRAARYILGASILLYGINLGHLGFLASGKAE
                     EAIQDVESILRGEYSVQTHRLLDGRISRDGHDTYSLYALNDLVLAKGALARVMHIEVH
                     IGDKLLNTLPADGIIVSTPTGSTAYALSAGGPIIPPHVPCMLVVPICAHTLYSRPVVA
                     GEDDTVTLVPRGNHRDLLLTQDGQLGYEILPGDRIEISLSRTQKIDVLLLPSRNYFDL
                     LQEKLRWGQGIVTFEKE"
     gene            2445..>3996
                     /locus_tag="P5201_04380"
     CDS             2445..>3996
                     /locus_tag="P5201_04380"
                     /inference="COORDINATES: protein motif:HMM:NF014516.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HRV97801.1"
                     /translation="MIEEISIKNVGGIASISMRLETGFTVITGESGAGKSSLVRALEL
                     IGGKRSQALFIRSGEEEASVEAVFSGSAERVSKTLQPRDEDGLLFARRILSRGGRNRC
                     FFQDKATPFSTFASLMNDRMRIQSQFAQIELLDPKRQMDILDFCCGAHAEHLRAALEN
                     TFDEALRCDREVRAVKSKEQELKLRFRDAEAIVEALRPLQISPGCDLLWERELDEKNR
                     TFQQARKVRENLLELTGGASNDGMIDRLESCALELIRRLFLEKTDVEASFNEGLSFLH
                     SFISSIEEKASALSPEKIERERDLIEKKLGILRKIKRTTGTRTAEELVSWTSDAQEAI
                     GWLREEGRLSSELLLCAKKLRREASSLAMELRALRQRAADSLEKEVNRHLQELGMAES
                     VFSVKLLPLDKIRSSGADEISFTLSSGGSEEMPVNKSASGGELSRILLALQLALPEET
                     LPPTLIFDEVEAGLGGKAALLAGYKLLALSRKTQVILVTHEATIASLADHHFLVKKTT
                     DCVEVKKLE"
BASE COUNT          941 a          991 c         1139 g          925 t
ORIGIN      
        1 gctggaggat tacggccggt agtgtttatc tactcaacat tcctccagcg agcaatggat
       61 cagctcgtcc atgatatcgc tatgcagaat ctcccggtta ccatcgcagt tgaccgttcg
      121 gggctcatcg gcgacgacgg agagacacat caaggattgc tcgacatttc atggtcgcgt
      181 tccgtacctc accttgtcgt ggcggctcca agggacgaga tagggctttc agcgctcttc
      241 gatctggggc ttcgtcgaaa cgggccgatg atgatccgct tccccagggg cgaggcgatt
      301 ccctcccttc acaggattcg gggcgaagtt tttctggacg accttcctgt tccggaaatc
      361 ctggaaaagg gaactgagtg cgcgctcctg gggtacggaa agaccgtcga actgatgctg
      421 gatgcgcgcg aaataattgt tcgcgacggt ggcgttcccc cttctgttgt ggacatcaaa
      481 tgtgtcaaac ccatgtgcgt tccggtaatg gaggggctct tgaaaagcca tgaacttgtc
      541 atcgttgccg aagacggtta caaagagtgc ggagtaggcg aggcgatcgc gtcccttgcc
      601 gaaaaactca ggccgtcgtc ccgaacagcc atggtgcttc cgatgggcat ccccgatctt
      661 ttcgttcccc aggggacggt ttctgaacag gccgagtact gcggccttac cccccgtaaa
      721 gtggtgacaa tgtatgaaga gtatcaaaaa agaacgcatc gacaaggttc tggtatccag
      781 ggggttcgtt gaaaccagaa caaaagcgca aagccttctg atggccggga aagttacagt
      841 cgatgggaat cgggtggaca agccgggcgc gcttgtcttt cctgacgcgg ctgtggaagt
      901 gatcggaacc ggaaacgaat gggtcagccg cggcgcactc aaactgctga aagctctcga
      961 tgtctttcct tttgacccca aagacagaat ttgcgtcgat atcggcgcct caacgggagg
     1021 gttcactcaa gttctccttt caaggggcgc tcttcgggta tattccgtcg atgtcgggta
     1081 tgggcagttg gcttggtcgc ttcggagcga tgagagagtc gttgtaatgg aacgtacgaa
     1141 tgccaggaat ttgcttccgg acaattttcc tgatataccc gacagggcgg ttgctgatgc
     1201 gtctttcatt tctctgaagt tattgctgcc ggctatggat acaattcttt ccccccaagg
     1261 agaagcggtt gtccttgtta agccgcagtt tgaagtcggt aaagggaaag ttgggaaggg
     1321 aggcgtggtc cgttcgagtg aggatcacat cgtcgttttg gaggagattc tgaatttctg
     1381 tttacgggaa acgacgttca cgccatgcgg tataacattt tcacccatta ccggtcctat
     1441 ggggaatatc gagtatctcc tctatctctc gaagtcagac tcggagcctt cgattccaca
     1501 cccagtctcg atagttgaag aagcacatag ttttttcacg aaaggacggg cggcatcatg
     1561 aacactccca atattggcat gcttgtcaat acgcgaaaac ccggggcgat aaaaatggca
     1621 aggatgcttt tgcagtgggg gatggacaaa ggaatcgcgt tcctcttccc tccgcatgag
     1681 gcgtcggtgc tgggagtcca gggggtgagc gacgaagagt ggaaagagac tgtcggaatc
     1741 gccttggtta tcgggggcga cgggacgttt ttgcgagctg cccggtatat tttgggcgcc
     1801 tcgattctcc tctacggaat caacttgggg catctcggtt ttctggcttc cggaaaggcc
     1861 gaggaggcga tacaggatgt ggaaagcatc ctccgcgggg agtactccgt gcagacgcac
     1921 agattacttg acggcagaat ctcacgagat ggacacgata cctattcctt gtacgctctc
     1981 aatgatcttg tcctggctaa aggagcgctt gccagggtaa tgcatattga agtgcacatt
     2041 ggcgacaaac ttctgaacac gctcccggcc gacggcataa tcgtgtcgac gccgactgga
     2101 tcgaccgcgt atgccctctc cgcaggcggt ccgattatcc ccccgcacgt tccgtgcatg
     2161 cttgtcgtgc caatatgcgc tcatacgctc tattccagac cagtagtcgc aggagaggat
     2221 gacacagtta ccctggttcc cagaggaaat cacagggatt tgcttcttac tcaggatggt
     2281 cagctaggct atgagatcct tccgggggat cgtatagaaa tctcgttgag ccgcacccaa
     2341 aagatcgatg ttcttctcct cccgtcacgg aattacttcg atctgcttca ggagaaactc
     2401 cgctggggac agggcattgt cacctttgaa aaggagtgac ggggttgatc gaagagatct
     2461 ccattaagaa cgtaggaggt atagcctcca tatcgatgcg cctggagacc ggcttcacag
     2521 tgatcaccgg agagagcggc gccggaaaga gcagccttgt cagggctttg gaactgatcg
     2581 gcgggaagag gagtcaagcg ctttttattc gctccggcga ggaagaggcg tcggtggagg
     2641 ccgtcttttc cggatctgcc gaacgagttt cgaaaacgct gcaaccgcga gacgaggacg
     2701 gactgctctt tgcgcggcgt attctttcaa gaggaggaag aaaccgatgt ttctttcaag
     2761 acaaagcgac tcccttctct acatttgcat ctttaatgaa cgacaggatg aggattcaaa
     2821 gccagtttgc ccaaatagag cttcttgatc caaagcgtca gatggatatt ctcgacttct
     2881 gctgcggcgc tcatgctgag catttgcgtg ccgcgctcga aaatacattt gatgaagcgc
     2941 tccgttgcga tcgggaagtg agagcggtga aatcgaaaga acaggagttg aagctgcgtt
     3001 ttcgcgatgc ggaggcaata gtagaagctc ttcgcccgtt gcaaatctcc ccgggttgcg
     3061 acctcctttg ggaaagagag cttgatgaaa aaaaccgcac cttccaacag gcccgaaaag
     3121 tcagggaaaa tctccttgaa ttgaccggag gggcttccaa cgacggaatg atcgacagac
     3181 tcgaatcgtg tgcgctggaa ctcatacgcc gtctctttct ggagaagacc gacgtcgaag
     3241 cgtctttcaa cgaagggttg tctttcctgc actcttttat cagctccatc gaagagaagg
     3301 cctccgctct ctcaccggaa aaaattgagc gcgaaaggga tctcattgag aaaaaactag
     3361 gaatacttcg gaagattaaa cgaacgacag gaacccgcac cgccgaagag cttgtttcct
     3421 ggacttcgga cgcgcaagag gctatcggct ggttgaggga ggaagggcgt ctctcttcgg
     3481 agctgcttct atgtgcgaaa aaattacgcc gcgaggcaag ctcattggcg atggagctgc
     3541 gcgcactccg gcaacgggca gcggattcac tggaaaaaga agtgaatagg caccttcagg
     3601 agctcggcat ggccgagagc gttttttcgg tgaaactttt gccgttggac aaaatacgca
     3661 gcagcggggc tgacgaaatt tcatttactc tctcctcggg aggctctgaa gaaatgcctg
     3721 tcaataaatc tgcttccgga ggcgaactga gtagaatatt gctcgcgctc caacttgcgt
     3781 tgcctgagga aacgctcccc ccgactctta tcttcgatga agtggaggcc gggcttggcg
     3841 gaaaggctgc gcttctggcc ggatacaagc tgctcgctct ctccagaaaa acacaggtga
     3901 tccttgtaac gcacgaggca acgattgcat cgctcgcgga tcatcacttt ctcgtcaaga
     3961 aaacgacgga ttgtgtagaa gtcaaaaagc tggaaa
//
LOCUS       DAOTIL010000154         3985 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminobacteriaceae bacterium isolate AMR_MDS_3026
            NODE_26822_length_3985_cov_3.391858, whole genome shotgun sequence.
ACCESSION   DAOTIL010000154 DAOTIL010000000
VERSION     DAOTIL010000154.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868050
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminobacteriaceae bacterium (wastewater metagenome)
  ORGANISM  Aminobacteriaceae bacterium
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae.
REFERENCE   1  (bases 1 to 3985)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3985)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:19:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,224
            CDSs (total)                      :: 3,198
            Genes (coding)                    :: 3,184
            CDSs (with protein)               :: 3,184
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3985
                     /organism="Aminobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_26822_length_3985_cov_3.391858"
                     /isolate="AMR_MDS_3026"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:3034609"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..253)
                     /locus_tag="P5201_04385"
     CDS             complement(<1..253)
                     /locus_tag="P5201_04385"
                     /inference="COORDINATES: protein motif:HMM:TIGR01409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocation signal
                     domain-containing protein"
                     /protein_id="HRV97802.1"
                     /translation="MDRREFLKRSLAAGVGGAALLVPGSVKRVFADSGKLPGLAAVRG
                     GEPGEMFDRGIAALGGMERFVKKGQTVAIKPNMSWDVAPE"
     gene            complement(412..657)
                     /locus_tag="P5201_04390"
     CDS             complement(412..657)
                     /locus_tag="P5201_04390"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRV97803.1"
                     /translation="MSRAFIKENEDQESYLEWQKLLRDREELLRILEKKKKYLLEDPA
                     AGTIPEEKRHEMIAKYDEEAEEVRRLLDEMLAETKIP"
     gene            complement(928..2109)
                     /locus_tag="P5201_04395"
     CDS             complement(928..2109)
                     /locus_tag="P5201_04395"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005659558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006635 - fatty acid beta-oxidation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiolase family protein"
                     /protein_id="HRV97804.1"
                     /translation="MGKPVLLSACRTPGGKFGGALKDLAAPDLGAIALAEAVKRSGVS
                     PEDIGEVIMGNGWQAGIGANPARIAMFRAGLPQSIPAFTINKRCGSSLKTAMLAADKI
                     RLGDMVAVAAGGMESASNVPYILPEARWGYRMGEKKALDALHKDGFHCPLSDMLMGAT
                     AEVLVEERSITREEQDEYALNSHRKAVAAIEGGFFAEEIVPVPVKDRKKGTVMVDTDE
                     IPRSDTSMESLGRLPALFKKDGTITAGSSSALCDAGSAYIVADSDWAAANGFTPLAEI
                     LGYASGALDALHMGLGPVVAMPKALQHAGMSLEDMEIIEINEAFSAQILACHRDMPFD
                     MEKLNPCGGAIALGHPIGATGGKILATLIYSLKRQKKEFGIVSACIGGGQGVAMVLRN
                     L"
     gene            complement(2135..2809)
                     /locus_tag="P5201_04400"
     CDS             complement(2135..2809)
                     /locus_tag="P5201_04400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008522114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008410 - CoA-transferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacid CoA-transferase subunit B"
                     /protein_id="HRV97805.1"
                     /translation="MLPVLDEEVVRHRIAQRIALDFEDGAVVNLGIGIPTLVSDYLPE
                     GVHVMLQTENGVIGAGPKPEVDDLRLIGAGGRCLSLLPGSSIVASDMSFGLIRGGHLD
                     ATVLGALEVDSHSNLANWMIPGKMVPGMGGAMDLVTGARAVYVATTHCDKKGRPKLVK
                     ECTLPLTGANVVSVVVTEFCLIRKIDGKMVLCEIAPEVTPEELKANTTMEYDVAPDLA
                     PMKGIE"
     gene            complement(2793..3500)
                     /locus_tag="P5201_04405"
     CDS             complement(2793..3500)
                     /locus_tag="P5201_04405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013048597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008410 - CoA-transferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA transferase subunit A"
                     /protein_id="HRV97806.1"
                     /translation="MAHKLIKPVMSAEEAVTHIQPGSSLMVGGFNYAGVPYTLIDALV
                     AQGTDNLHLISNDTLYANDRHPEGIGHGKLVVNGQCRKVTASHVGLNRETQRLFNEGK
                     MELELVPQGSFVERIRCGGFGLGGFLTPTGVGTIHEEGKQVIEVDGKKFILELPLKAD
                     VAIIRGYKADRWGNLTYYGTGRNFNPAMATAADLVIAEVDMILAQGDIDPNDVVTPGM
                     LVDILVLKGDGYYASRT"
     gene            complement(3519..>3985)
                     /locus_tag="P5201_04410"
     CDS             complement(3519..>3985)
                     /locus_tag="P5201_04410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005659552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF3394 domain-containing protein"
                     /protein_id="HRV97807.1"
                     /translation="LMSAHLFCLYFGVLADVTPPVALATYAAAGIAKSNAIKTGFTAL
                     VTAMAGFLVPYMFVYNNHLLFQGNLLQIAFSFGTALMAIIGLAAGVQGYYFSVLSIFE
                     RVLLLIVPFLLIEPNIITDVIGLVILVSVYLLQRRKAAKHAKEKTVSDAAQS"
BASE COUNT          794 a         1283 c         1048 g          860 t
ORIGIN      
        1 gctcgggcgc gacgtcccag gacatattcg gcttgatcgc aacggtctgc cctttcttca
       61 caaaacgctc cattcctcca agggccgcga tgccgcgatc gaacatctcc ccgggttctc
      121 ctccgcgaac ggccgccagt ccgggcagct ttccagagtc cgcaaagacc ctcttcacgc
      181 ttcccggaac cagcagcgcc gcgcctccta cccccgcagc aaggctcctc ttcaaaaact
      241 ctctccggtc cattctcaca cctcctcgaa cagctcttgt tatataatag cacgttcgcg
      301 atgtacattt attcgacatt cctgagtaga gtgcaaaaag ccgtcgtaaa tcagggagaa
      361 tgcatatccc tgtcttacga cggctttttg cagcgactct caggcgaaaa atcagggtat
      421 cttcgtctcc gccagcatct catccagaag acgccgaacc tcctcggctt cttcatcgta
      481 tttcgcgatc atctcatggc gcttctcttc gggtattgtc ccggcggcag gatcttccaa
      541 aagatacttc tttttcttct caaggatacg cagcagctct tccctgtccc gaagaagctt
      601 ctgccattcc aagtagctct cctgatcctc gttctcttta ataaacgctc tgctcacagc
      661 tatcccccct ctcaaacgat gaaaagcgca acaatacaca aggccacgga aaggatccgg
      721 ggccctgttg gctcttcatg atgacgacag aaaaaactgc gttttcgatt ctattatcgc
      781 ccaaccgcga aacttttgca agcgcctccg catccgaagc gcccgctcgt accgacgatc
      841 gcgaagcgtg gaataaaaag cgcgcgggac cggcgtacac aaacggcccg cgcgctttct
      901 tcgaagagtg ccggaactct ggcgtactta cagattccga agcaccatcg ccactccctg
      961 gccgccgccg atacacgcgc tgacgatccc gaactccttc ttttggcgtt tcagcgagta
     1021 gatcagcgtc gcgaggatct ttcctccggt cgcccctatc gggtgaccga gcgcgatcgc
     1081 gcccccgcag ggattcagct tctccatgtc gaacggcata tccctgtggc atgcgaggat
     1141 ctgcgcggag aaggcctcgt tgatctcgat gatctccata tcctcaagcg acattccggc
     1201 gtgttgaagc gctttcggca tggccacgac aggcccgagc cccatatgaa gggcatcgag
     1261 cgcgcccgaa gcgtatccga gtatctccgc caaaggtgtg aagccgttcg ccgcagccca
     1321 atcgctgtcg gctactatat acgcgcttcc ggcgtcgcac agggcggagc tggaacccgc
     1381 cgtaatcgtc ccgtcctttt tgaagagcgc gggcagcctc cccaacgatt ccatcgacgt
     1441 gtcgcttctg ggaatctcgt cggtatcgac catgacggtc cccttcttcc tgtccttcac
     1501 cggaacggga acgatctcct cggcgaagaa accgccttct attgccgcga cggccttcct
     1561 gtggctgttg agcgcgtatt cgtcctgttc ttccctggtg atggagcgct cctcgacgag
     1621 cacctccgcc gtcgccccca tgagcatatc cgaaagaggg cagtggaatc cgtccttgtg
     1681 gagcgcgtcg agcgccttct tctcgcccat ccggtacccc catcgggcct ccggaagaat
     1741 gtacggcacg ttggaagcgc tctccatacc gcccgcagcc acggcgacca tgtccccgag
     1801 gcgaatcttg tccgcagcga gcatcgccgt cttgaggctg gagccgcagc gcttgttgat
     1861 cgtgaacgca ggaatggact gagggagtcc ggcgcggaac atcgcgatac gcgccggatt
     1921 cgccccgatg cccgcctgcc agccgtttcc catgatgacc tcgccgatat cctccggaga
     1981 tacgcccgac cgcttcaccg cctcggcaag agcaatcgct cccaggtcgg gggcggcaag
     2041 atccttgagc gccccgccga atttcccgcc gggtgttctg cacgcgctta atagtaccgg
     2101 tttgcccatt tttcttcctg tcctttcgta ttcgctactc gatgcccttc ataggcgcga
     2161 gatccggagc gacgtcgtac tccatcgttg tgttcgcctt cagctcctcg ggagtgactt
     2221 ccggcgcaat ttcacagagc accatcttcc cgtcgatctt acgtataaga caaaactcgg
     2281 tgacgacgac gctgaccacg tttgctccgg taagcggcaa agtgcactcc ttcacgagct
     2341 tcgggcgccc cttcttgtcg cagtgcgtcg tggcgacgta caccgctctg gcgccggtca
     2401 ccagatccat cgccccgccc atgccgggaa ccattttgcc ggggatcatc cagttggcga
     2461 gattcgaatg actgtccacc tcgagggcgc cgagcaccgt cgcgtcgaga tgacctcccc
     2521 tgatgagccc gaagctcata tcactggcaa cgatggacga accgggaaga agcgagaggc
     2581 agcgcccccc cgcgccgatg agccgaaggt cgtccacctc cggtttagga ccggctccga
     2641 tcacgccgtt ctccgtctgc agcattacgt gcacaccctc gggaaggtaa tctgaaacca
     2701 gcgtcggaat gccgatgccg aggttcacca cagcgccgtc ctcgaaatcg agcgcgatcc
     2761 gttgtgctat tctatggcgg acaacttctt catcaagtac gggaagcata gtatccgtct
     2821 cctttcagaa cgagtatatc gacgagcatt ccgggggtga cgacgtcgtt cgggtcgata
     2881 tctccctgag ccaggatcat atcgacctcc gcgatcacca gatcggccgc cgtggccatc
     2941 gccgggttga agttccgtcc cgtgccgtaa taggtcaggt ttccccagcg gtccgccttg
     3001 tacccccgga tgatcgccac gtccgccttc aggggaagtt cgagaataaa cttttttccg
     3061 tcgacctcga tgacctgctt cccctcctcg tgaatcgtcc cgacgccggt cggcgtcaga
     3121 aaaccgccga ggccgaaccc tccgcagcgg atccgttcca cgaaagaccc ctgcggaacc
     3181 agttcgagct ccatttttcc ctcgttgaag agtctctgcg tttcccggtt cagcccgacg
     3241 tgcgacgcgg tcacttttct gcactgaccg ttcacaacaa gcttgccgtg gcctattcct
     3301 tccgggtgcc ggtcgttggc gtagagcgta tcgttcgaaa taagatgcag gttgtccgtc
     3361 ccctgcgcca cgagcgcgtc gatcagggta tagggaaccc ccgcatagtt gaatcctccc
     3421 accatcagcg aggaaccggg ctgtatgtgc gtcactgcct cctcggcgga catgacgggc
     3481 ttgatgagct tatgcgccat gaagagatga cacctccttt aagattgtgc agcgtctgaa
     3541 acagtttttt ctttcgcgtg cttcgccgct ttgcgtcttt gcagtagata gacgctcaca
     3601 agaatgacga gcccgatcac gtctgtgatg atattcggtt cgatgagcag gaagggaaca
     3661 ataagcagaa gcactctctc gaaaattgag aggaccgaga agtagtaccc ctggacaccc
     3721 gccgcgagac cgatgattgc catgagcgcc gttccaaagg agaacgcgat ttgcagcagg
     3781 ttcccctgga agagcaggtg gttattgtat acgaacatgt aagggacgag gaatcctgcc
     3841 attgccgtaa ccagcgcggt gaatcctgtc ttgatcgcgt tgctctttgc gattccggca
     3901 gcggcgtagg tcgccagcgc taccggcggc gttacatccg cgagcacgcc gaaatagaga
     3961 cagaagagat gcgccgacat gagcg
//
LOCUS       DAOTIL010000155         3964 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminobacteriaceae bacterium isolate AMR_MDS_3026
            NODE_27010_length_3964_cov_3.055001, whole genome shotgun sequence.
ACCESSION   DAOTIL010000155 DAOTIL010000000
VERSION     DAOTIL010000155.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868050
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminobacteriaceae bacterium (wastewater metagenome)
  ORGANISM  Aminobacteriaceae bacterium
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae.
REFERENCE   1  (bases 1 to 3964)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3964)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:19:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,224
            CDSs (total)                      :: 3,198
            Genes (coding)                    :: 3,184
            CDSs (with protein)               :: 3,184
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3964
                     /organism="Aminobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_27010_length_3964_cov_3.055001"
                     /isolate="AMR_MDS_3026"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:3034609"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..219)
                     /locus_tag="P5201_04415"
     CDS             complement(<1..219)
                     /locus_tag="P5201_04415"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRV97808.1"
                     /translation="MIIVRISLLGVYRRRLCSLALFLSLFFVSASEGAVLFVDAGAAG
                     ANDGTSWTDAYTDLQTALTTAADDDEIWV"
     gene            complement(533..1945)
                     /locus_tag="P5201_04420"
     CDS             complement(533..1945)
                     /locus_tag="P5201_04420"
                     /inference="COORDINATES: protein motif:HMM:NF023990.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3754 domain-containing protein"
                     /protein_id="HRV97809.1"
                     /translation="MEEQKERGSFIPVRTDDLVRALCEERALSGEQREKFKKVANLVE
                     ALYHYDFHDTLEKARGAYHFFNPDADTLKRYAPDTDPAKKFDAMVKALEKILVAANYR
                     EFPLDELNALMTKAAPEGLDVQVDLARYEKILIYRRGSKLAPKARNLLSRTKFFRKKD
                     APAEPETEVSPPTHDQEEIIQRLLILIKLKSSEDMEQFYDSFIETAGETGQERARRMA
                     EKALFGKKEPKLHGASSGTPAIFLKVFKNVPVSALETLFPDVTVRMTLLDKGLILLPL
                     VGGILSVVNRVIPALLLIGTIIAAVVAGRAIDWGEFRNHLFPILTAFSIVGVITFKIF
                     SKYKITKEKHQAKLMKTLYFHNLDNNAGVFDFLVQEAEEEECKEALLAYYFLLTERNA
                     NGKPFTEEELDRRIEQWMGERFGMTLDFEVDDALRKLEEKKLLVKDGDRYRVPPIDET
                     LIRLDELWDGVFAYSNERKE"
     gene            2184..>3964
                     /locus_tag="P5201_04425"
     CDS             2184..>3964
                     /locus_tag="P5201_04425"
                     /inference="COORDINATES: protein motif:HMM:NF012350.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IC family P-type ATPase"
                     /protein_id="HRV97810.1"
                     /translation="MQKQFKGLTSQEVLESRRTHGENVLTPPVRESWWKLYLEKYDDP
                     VIRILLIAACIAFLVGLAHNEYVEAVGIVVAVFLATTMAFVNEYKAGREFDILNQVND
                     DVEFKVIRDGEYRSVSKKELVVGDILLLETGEEVPADAVVEEAVALQVDESTLTGESK
                     AVSKFSEDDPRGAEKREAATYPPHRVYRGTMVQDGHGVFCIESVGDATEIGKTAREAS
                     AVSEERTPLSRQLDGLSKVIGVVGFGIAAVLFAVLSVHAALVGEIVMERGQWIFALIL
                     IASVLVALVRVWLPIVYDFFDVIRRPKTRPGWLQQEGITAWLKPLLYGAVFFALASGA
                     AWTLGALPSSPSNFLDADAGRRFLQFFMIAITLIVVAVPEGLAMSVTLSLAYSMRKMT
                     ASNNLVRKMHACETIGAATVICTDKTGTLTKNKMHVHEVRVPSLNASPELLYEAMAAN
                     STANLSKSGGETEALGNPTEGALLLWLDERGEDYKALRDSFDVLDQLTFSTERKYMAT
                     FGKGRDGGVVLHAKGAPEILLETCSSILSESGEEALTDGVKEEILSSIGSFQARGMRA
                     LGFAVRRELPEAGEVSAESLMEGLTWLG"
BASE COUNT          751 a         1121 c         1255 g          837 t
ORIGIN      
        1 cacccagatt tcgtcgtcgt cggccgcagt tgtcagtgct gtctgaagat cggtataggc
       61 gtcggtccat gatgtgccgt cgtttgctcc tgctgctccc gcgtcgacaa aaaggaccgc
      121 cccttccgat gccgatacga aaaagagcga cagaaaaagc gccagagaac acaaacgccg
      181 cctgtagact ccgagtaaag aaatacgcac tatgatcatc tcctatctcc ttttgtttcg
      241 ctgaactgcg ggagcgatac tcctgctgaa cagcggccgc ttttagcgtg cacaacaaca
      301 attggagcat tcgagcgctc cagtgcgttt attgtagaac tgcatccgca gaaaaaagag
      361 aggtggtttt tcccacccct ctttttagat gcagcaatag caatagtctt cgcaataatg
      421 ctcaatcggc tctgtaaaga tgagccggag gaagtgtgtg agcagaaacc tttggacgag
      481 ggggcaagaa taatctgttt cggcgatcat gaagctaccg ggcttgcttc ttctactctt
      541 ttctttcgtt gctgtacgca aaaacgccgt cccacagctc gtcgaggcgg atcagcgtct
      601 cgtcgatcgg cgggactcgg taccggtcgc cgtcctttac gaggagcttc ttctcctcca
      661 gcttgcggag ggcgtcgtcc acttcgaagt ccagcgtcat cccgaagcgt tctcccatcc
      721 actgctcgat gcggcggtcc agctcttctt ccgtgaatgg tttgccgttc gcgttccgct
      781 ccgtcagcag gaagtagtag gccaacagcg cctctttgca ctcctcctct tcggcctcct
      841 gcacgagaaa gtcaaagacc ccggcgttgt tgtccaggtt gtggaagtag agcgtcttca
      901 tcagctttgc ctggtgcttc tccttcgtga tcttatactt ggagaagatc ttgaaggtga
      961 tcacgccgac gatggagaac gcagtgagaa tggggaaaag atgattgcgg aactcgcccc
     1021 agtcgatcgc gcgcccggcg accaccgcgg cgatgatcgt gccgatgagg agcagcgcgg
     1081 gaatgacgcg gttgactacg ctgagaattc cgcctaccaa ggggaggagg atcaacccct
     1141 tgtcgagcag cgtcatccgg acggtcacat ccgggaagag cgtctccagc gcggagacgg
     1201 ggacgttctt gaagaccttc aggaagatgg cgggcgtccc cgaggaagcg ccgtgcagtt
     1261 tgggctcctt cttgccgaag agggctttct ccgccatgcg gcgcgcgcgt tcctgtccgg
     1321 tttcgccggc ggtttcgatg aaagagtcgt agaactgctc catatcctcg ctgctcttca
     1381 gcttgatcag gatcagaagg cgctggatga tctcctcctg gtcatgcgtc ggaggcgaga
     1441 cctcggtctc cggttccgca ggcgcgtctt tcttccggaa gaattttgtc cgagacagca
     1501 agttacgagc cttcggggca agtttcgacc cccttcggta gatcaggatt ttctcgtacc
     1561 tggcaaggtc gacctgtaca tccagcccct cgggcgcggc ctttgtcatc agtgcgttga
     1621 gttcgtcgag gggaaactcc ctgtagtttg cggcgacgag gattttttcc agcgccttca
     1681 ccatcgcatc gaacttcttt gcggggtcgg tatccggtgc gtagcgtttc agcgtgtcgg
     1741 cgtcggggtt gaagaagtga taggctcccc gcgccttctc cagcgtatcg tgaaagtcgt
     1801 agtggtagag cgcctcgacg aggttcgcaa cttttttgaa cttctcccgc tgttccccgg
     1861 agagcgccct ctcttcgcac agagcccgca cgaggtcgtc ggtgcgcacg gggatgaacg
     1921 agccccgctc tttctgttcc tccatcgtaa ctcctcctgt actcggcttt tctgtgcgga
     1981 cgtcccgctg cggggcgcat ccgcgcagaa agtatagcac ggcgttcacg tttcttcagg
     2041 cgtttcgaga agggggtatt tcgatcgcgc attctcttcg gtgacgaaga agcgcgccct
     2101 gaaagcgtat aatggaaccg ttgcgcaaag agacgggaag acggccttca caggtcgtcc
     2161 gcgtggatat ttggaggtgt gctatgcaga aacagttcaa gggtttgacc tctcaggagg
     2221 ttctggagag caggagaacg cacggagaaa acgtgctcac cccgccggtg cgggagtcat
     2281 ggtggaagct gtacctggag aagtacgacg atccggtgat ccggattttg ctcatcgcgg
     2341 cgtgcatcgc cttcctggta gggcttgccc ataacgagta cgtggaggcc gtcggtattg
     2401 tcgtcgcggt gttcctcgcc acgacgatgg cctttgtcaa cgagtacaag gcgggcaggg
     2461 agttcgacat actcaatcag gtgaacgacg acgtcgagtt caaggtgatc cgggacggcg
     2521 agtaccggag cgtttcgaag aaggagcttg tcgtggggga tatccttctg ctggagacag
     2581 gggaggaggt cccggcggac gccgtcgtcg aggaggccgt cgcgctgcag gtcgacgaat
     2641 ccacgctgac gggagagtcg aaggccgtct ccaagttttc cgaggacgat ccgaggggcg
     2701 ccgaaaaacg agaggcggcc acctacccgc cgcaccgcgt gtacagggga acgatggtgc
     2761 aggacgggca cggggtcttt tgcattgaga gcgtcggcga cgccacggag atcgggaaga
     2821 cggccaggga ggcctcggcg gtctccgagg agcgcacgcc gctgagccgt caactcgacg
     2881 gcctgagcaa agtgatcggc gtcgtcggct tcggcatcgc ggcggtgctc ttcgccgtat
     2941 tgagcgtcca cgccgcgctc gtgggagaga tcgtcatgga gcgagggcag tggatctttg
     3001 cgctcatcct gatcgcctcg gtacttgtcg cgctggttcg tgtgtggctg ccgatcgtct
     3061 acgacttctt cgacgtaatc cggcggccga agacgcggcc ggggtggctg cagcaggaag
     3121 ggatcacggc gtggctgaag ccgctgctct acggcgcggt gttcttcgcg cttgcatcgg
     3181 gcgctgcgtg gacgcttggc gcgttgccct cgtctccctc gaacttcctg gacgccgacg
     3241 cagggcggcg attcctccag ttctttatga tcgccattac cttgatcgtg gtcgccgtcc
     3301 ccgaggggct ggcgatgagc gtcacgctct cgctggcgta cagcatgcgg aagatgaccg
     3361 cctcgaacaa cctggtgcgc aagatgcacg cctgcgagac cataggcgcc gcaacggtaa
     3421 tctgcaccga caagaccggc acgctgacga agaacaagat gcacgtgcac gaggtgcgcg
     3481 ttccctcctt gaacgcctct ccggagctgc tctacgaggc gatggcggcg aattcgacgg
     3541 cgaacctctc gaagtcgggc ggggagacgg aagcgctggg gaatcccacg gagggggcgc
     3601 tgctgctctg gctcgacgag cgcggagagg actacaaggc gctgcgggac tcattcgacg
     3661 tgctggatca gctcacattc tccaccgagc ggaagtacat ggcgaccttc ggcaaaggac
     3721 gcgacggcgg cgtggtgctt cacgcgaagg gggcgcccga aatactgctc gaaacgtgct
     3781 cctcgatcct ctccgagtct ggagaagagg cgctgacgga cggggtgaag gaggaaattt
     3841 tgtcctccat aggctccttc caggccaggg ggatgcgcgc tctcggcttc gccgtgcgcc
     3901 gcgagcttcc cgaggccgga gaggtctctg ccgaaagcct tatggagggg ttgacatggc
     3961 tcgg
//
LOCUS       DAOTIL010000156         3962 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminobacteriaceae bacterium isolate AMR_MDS_3026
            NODE_27026_length_3962_cov_3.570770, whole genome shotgun sequence.
ACCESSION   DAOTIL010000156 DAOTIL010000000
VERSION     DAOTIL010000156.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868050
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminobacteriaceae bacterium (wastewater metagenome)
  ORGANISM  Aminobacteriaceae bacterium
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae.
REFERENCE   1  (bases 1 to 3962)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3962)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:19:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,224
            CDSs (total)                      :: 3,198
            Genes (coding)                    :: 3,184
            CDSs (with protein)               :: 3,184
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3962
                     /organism="Aminobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_27026_length_3962_cov_3.570770"
                     /isolate="AMR_MDS_3026"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:3034609"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..475
                     /locus_tag="P5201_04430"
     CDS             <1..475
                     /locus_tag="P5201_04430"
                     /inference="COORDINATES: protein motif:HMM:NF036996.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="asparaginase"
                     /protein_id="HRV97811.1"
                     /translation="PGRGPLAQFIGDKVDIIRQYKRSKVLEGSMTPARDVELLSVALG
                     GGERLLEILSSDEKRELDGLVIAGFGNGHVPPSWIPRIKKLLKDDVPVVITTRCPEGY
                     TREIPYSFEGSMSRLLEMGVLQGGSLRPAQARLKLAAGLGAGLRRSDLQQYLLEK"
     gene            638..1063
                     /locus_tag="P5201_04435"
     CDS             638..1063
                     /locus_tag="P5201_04435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013047948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HRV97812.1"
                     /translation="MKYAATDEIIAIRLSEGEDLADSLAHVCKACDVDSAVIVSAAGM
                     VSSVTFGWYNGRDYNTESVREIMELTSLSGNVSFRGGSLYPHLHGVFNEPDHAALSGH
                     LLQAIVFNNAELFLKPLATVHLGRSFDGSFEALSPEKKM"
     gene            complement(1123..1644)
                     /locus_tag="P5201_04440"
     CDS             complement(1123..1644)
                     /locus_tag="P5201_04440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005661532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbaK/EbsC family protein"
                     /protein_id="HRV97813.1"
                     /translation="MDSVIQHAQEEAFVIEESSSDPVEKVLRFLNERGFHGAVTYTEE
                     TIFTVEDASRVVGAQPEHILKSLVLLVDDRPALALMSGTNRVDVKKVRREAEAKKVRM
                     ADPEYVYEWSGFKIGGVPPVGYPELPEAFLDEDLFLYPVVWAAAGTDHSFFPIAPGDL
                     LRLAEGRKCSIKK"
     gene            complement(1648..2529)
                     /locus_tag="P5201_04445"
     CDS             complement(1648..2529)
                     /locus_tag="P5201_04445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013047946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HRV97814.1"
                     /translation="MDFRELQSLVEVMEKGSISAAAASLGISQPAVSKHIAKLEREMG
                     VKVFARGQKCSNLTVEGEVLYKFARKTISQLADIKRDFSDIADDVSGIVRISASSIPG
                     DYILPGILVEFRKLYPNIEVEVRISDSREAVEKLVTRESDISVTGHGRHASGFNTVPF
                     ARDELVLLVAPGHPLAERENITLRDLENMDLVGRVTGSSTSRIWEDAFKAHTGKGKHV
                     YLKFGHVNAVVEAVRNGAEGAVISRLAGEDVSGLVMIPFRPALMRTFSITYGTMTTKA
                     METLLEFLLERKTFKDE"
     gene            2720..3091
                     /locus_tag="P5201_04450"
     CDS             2720..3091
                     /locus_tag="P5201_04450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008521869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HRV97815.1"
                     /translation="MSLGLRIRTLRKALGLTQQGLAERTQVSRIYIQALESNRRTPSM
                     KLLSRLANSLEVEVQDLVKTMSTSPSGRLQLEEVFQAAPETEVWYRSKRLKPKELRFV
                     QSLIDAAIERWEEEDRADGSV"
     gene            3118..3549
                     /locus_tag="P5201_04455"
     CDS             3118..3549
                     /locus_tag="P5201_04455"
                     /inference="COORDINATES: protein motif:HMM:NF017885.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ImmA/IrrE family metallo-endopeptidase"
                     /protein_id="HRV97816.1"
                     /translation="MGSRKQSQSTEFTFPSPPDFLPEWALREGGDWAGLDEFSLLVKA
                     EERTGKILELEYHDLPFGTWGVHIVRGRRGLIFINKRLSPYWRRFALFHELYHFLEHN
                     GGMELWTRTATPLSSFEHQADLFAWGALRKEWGRCWAHAFP"
     gene            3525..>3962
                     /locus_tag="P5201_04460"
     CDS             3525..>3962
                     /locus_tag="P5201_04460"
                     /inference="COORDINATES: protein motif:HMM:NF015983.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HRV97817.1"
                     /translation="MLGPRISMSAALPKIPFFLPMDSCPRRCVYCHQGEITGISEVPS
                     PDAVQQTLSRLQAPHEVCYFGGSFTCFPEERRRAYMEAVFAAPEGSCVRFSTHPICLS
                     PDILDSLAPFPVSMIEVGISSLDDDVLNACNRGYTGEFAIAAMR"
BASE COUNT          980 a         1047 c          983 g          952 t
ORIGIN      
        1 acccggaaga ggcccgcttg ctcagtttat cggcgacaag gtggatatta tcaggcagta
       61 taaacgatct aaagttcttg agggatccat gacgccggcg cgtgatgtcg aacttctttc
      121 cgtcgccctt gggggcggtg aaaggctcct tgaaattctt tcatccgacg agaaacgaga
      181 actcgacggt cttgttattg caggtttcgg taacggccat gtgcctcctt catggattcc
      241 gcgaataaaa aaacttctaa aagatgacgt gcctgtagtg ataacaacac gatgcccgga
      301 aggatacacg cgtgaaattc cgtattcttt tgagggaagt atgtcgcgcc tcttggagat
      361 gggggtcctt caaggcggtt ctttacgtcc ggcacaggca aggctcaagc ttgccgccgg
      421 actcggcgcg ggactccgtc gatcggatct gcagcaatac cttcttgaga aataaagcaa
      481 aaagacaact tcgtttttta gccgcattcg gttgaaagag gcattcttct gagaagtggc
      541 ggagagaatc aatacatggt agaatattga aggaatacaa gagaaccgct ttccgccggg
      601 caatgcacgc cggaaatatg aaaggaggac gagaaacatg aaatatgccg ctaccgatga
      661 aattatcgcg atacgtcttt cggaaggaga agatctcgca gattctctgg cgcatgtctg
      721 caaggcatgc gacgtcgatt cggcagttat cgtctccgca gcgggaatgg tgtcttccgt
      781 aactttcggg tggtacaacg gacgtgatta taatacggaa tcagtgcgtg aaataatgga
      841 acttacctcg ctcagcggca atgtaagctt ccgcggaggc agtctctacc cgcatcttca
      901 cggcgttttc aacgaacctg atcatgccgc gctttccgga catcttctcc aggccatcgt
      961 attcaacaac gcggaacttt ttttaaaacc gcttgcaacg gttcatcttg gacgctcttt
     1021 cgacggctct tttgaagccc tttcccctga gaaaaagatg taacctcgca cacagtgcga
     1081 cccaaaaagt tcctcccgaa aacgggagga actttttttc gcttactttt tgatgctgca
     1141 cttcctgccc tctgcgaggc gcaacaaatc accgggagct atgggaaaaa acgaatgatc
     1201 cgttcctgcg gcagcccaga caacggggta caaaaagaga tcttcatcaa gaaacgcctc
     1261 aggcaactcc ggatatccca caggaggcac gcctcctatt ttgaaaccgg accactcgta
     1321 aacatactcc ggatctgcca ttcgaacttt tttcgcttcc gcttctctgc gtactttctt
     1381 aacatccacg cggttcgttc cggacataag agcaagcgcc ggccgatcgt ctacaagaag
     1441 caccaagctt ttcaaaatat gctccggttg ggcgccgacg accctggatg cgtcttccac
     1501 agtaaagatt gtttcttccg tgtatgtgac cgctccatga aaacctctct cattcaaaaa
     1561 gcggaggacc ttttccaccg ggtcggaaga cgactcttcg ataacgaagg cctcctcttg
     1621 tgcatgctga attacagagt ccactttcta ctcgtccttg aaggtttttc tctcaagcaa
     1681 aaactcgaga agcgtctcca tcgcctttgt cgtcatggtt ccgtacgtga tcgaaaatgt
     1741 ccgcataagg gcgggacgga acggtatcat tacaagcccg gagacgtcct cgcccgcaag
     1801 acgggagatg actgctccct cggccccgtt tcgtacagct tcaacaaccg cattgacgtg
     1861 accgaatttg agatagacat gcttcccttt tccggtatgg gctttgaatg catcctccca
     1921 gatacgcgat gtcgacgaac cggtgactct tcccacaagg tccatgttct ccagatcgcg
     1981 cagagtgatg ttttcgcgct ccgcaagcgg gtgtccggga gcgactagaa gaaccagttc
     2041 atctctggcg aaaggaaccg tattgaatcc ggatgcatgt ctcccgtgtc ccgtaacaga
     2101 gatatcggac tcccgggtaa caagtttctc aacggcttct cgcgaatctg aaatgcgcac
     2161 ctccacttcg atatttggat agagtttccg gaactctaca agaataccgg gaagaatata
     2221 atcgcccggt atggagcttg cgcttatcct cacaataccc gatacatcat cggctatgtc
     2281 cgaaaagtcg cgtttgatat ccgcaagctg agagatagtt ttacgggcaa atttatacaa
     2341 cacttcccct tctactgtca ggttggaaca tttctgtccc cgggcaaata ctttgacccc
     2401 catctctctt tcaagcttcg ctatgtgttt gctgactgcc ggctgagaaa ttccgagtga
     2461 tgctgccgct gctgagatgc tccctttttc cataacttct accagacttt gcagttcacg
     2521 aaaatccaaa gcggatacct ccccaaagac aatatattct ttttccatcc aaacgccccg
     2581 acataaatat attctgaaag agaaagaact tcaagagcca aagatatttt tcttcaaaag
     2641 aaggcaaggg cttgaactta atgtaatcaa gtatataata atatatatcc aaagaaaaca
     2701 agacggagga gggatcgcga tgagccttgg actgaggata cgcactcttc gtaaggcctt
     2761 gggcctgaca cagcaggggc ttgcggagag aacacaggtg agcaggatat acatacaggc
     2821 attggaaagc aaccggagaa ctccttcgat gaaactcctc tcgcgccttg cgaattcact
     2881 tgaggtggaa gtacaggatc tggtaaagac aatgtcgaca tcgccttcag ggagattgca
     2941 acttgaagaa gtctttcaag ccgctcccga gaccgaggtc tggtacagaa gcaagagact
     3001 gaaacccaag gaactgcgtt tcgtccagag cctcatcgac gcggcaatcg aacgttggga
     3061 agaagaggac cgcgccgatg gaagcgtctg atccttcttc acgccggatt ttctgttatg
     3121 ggcagtcgga aacagtccca aagcactgaa ttcacgttcc cttcgccccc tgacttttta
     3181 cccgagtggg cgttgcgaga aggcggcgat tgggccggtc tcgatgagtt ctcgctcctc
     3241 gtaaaagccg aagaacggac gggaaagatt cttgagctcg aataccacga tctgccattt
     3301 ggtacatggg gcgtacacat agttcgcgga cgaagggggc taattttcat caataaacgg
     3361 ttgtcgcctt actggaggcg cttcgctctt tttcatgaat tgtaccattt tctggagcac
     3421 aacgggggaa tggagctgtg gacccgtacc gctacgccgc tctcgagttt cgagcatcaa
     3481 gcggatctct tcgcatgggg cgcgctgcgt aaggaatggg gcagatgctg ggcccacgca
     3541 tttccatgag cgccgctctc ccgaaaattc cattttttct tccaatggat tcctgcccga
     3601 ggcgttgtgt ctactgccac cagggagaga tcacaggaat ctccgaagtt ccttctccgg
     3661 acgcagtaca gcaaaccctc tcccggcttc aagctcccca tgaggtctgt tatttcggag
     3721 gtagctttac ctgtttcccg gaagagcgca ggcgcgcgta catggaagcc gtcttcgccg
     3781 ctccggaggg gagctgcgtt cgtttctcaa cgcacccgat ctgcctctcg cccgacattc
     3841 tcgacagcct tgcccctttc cccgtctcca tgatagaagt cggaatctcc tcgctcgacg
     3901 acgatgtact gaacgcatgt aaccgggggt acacaggaga gttcgcgatt gcagcgatgc
     3961 gg
//
LOCUS       DAOTIL010000157         3932 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminobacteriaceae bacterium isolate AMR_MDS_3026
            NODE_27300_length_3932_cov_3.781532, whole genome shotgun sequence.
ACCESSION   DAOTIL010000157 DAOTIL010000000
VERSION     DAOTIL010000157.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868050
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminobacteriaceae bacterium (wastewater metagenome)
  ORGANISM  Aminobacteriaceae bacterium
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae.
REFERENCE   1  (bases 1 to 3932)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3932)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:19:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,224
            CDSs (total)                      :: 3,198
            Genes (coding)                    :: 3,184
            CDSs (with protein)               :: 3,184
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3932
                     /organism="Aminobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_27300_length_3932_cov_3.781532"
                     /isolate="AMR_MDS_3026"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:3034609"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..375
                     /locus_tag="P5201_04465"
     CDS             <1..375
                     /locus_tag="P5201_04465"
                     /inference="COORDINATES: protein motif:HMM:NF015602.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar protein FlaG"
                     /protein_id="HRV97818.1"
                     /translation="KSMSPAFSVPSVEVMGWARKNYLEPSYPRKRYDEQRTFSDGRFR
                     DRVLRGDKSLERALDSFQEHAALHSRYLQFNIEEEADLVQVIVKESNDEEKIIRKIPS
                     DEIVRLIERRNEILGFLFDIVA"
     gene            complement(457..3048)
                     /locus_tag="P5201_04470"
     CDS             complement(457..3048)
                     /locus_tag="P5201_04470"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013047534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004748 - ribonucleoside-diphosphate
                     reductase activity, thioredoxin disulfide as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylcobalamin-dependent
                     ribonucleoside-diphosphate reductase"
                     /protein_id="HRV97819.1"
                     /translation="MSVSYKERFRSSFSSSAPAGGERLSENALWLLDQRYFARRYDPV
                     LRGIRKEKSFEEFARRVARTVACAETSYLAGGEEDLQWLRRLERNIFDDILNRRFLFN
                     SPCLFGAGAGLTVDPGMAERIYCDPEELTFEDYSLLHRSKTKGQQLFACFVIEVPDSI
                     DGIFDSVKDAAVISKYGGGVGGNFGHLRERSSVINGGIGGQASGPVSFMETWNTMGAV
                     VVQGGRRRAALMGMLFDDHPDIFDFIDAKVDEGKLPYFNISVCVSDKLMKSAQTGADF
                     NLVSRSNGSVVRSLNGAELWSKLCENAWKRGDPGIFFIDRANADNLLKLDETWRIEST
                     NPCGEQPLPNYTSCNLGSVNVEAFVERREDGNVRFDFAAFSDQVFRSMYYLDLVIDAC
                     SYPLEKIGERTRRIRPVGLGIMGLADAAILLRMRYGSERFKVFCRTLADSMGSAALCA
                     SVGMAEEMGKEPFPDAHLVEELFRRFAAEQGEELFPESWFASLDRESMQSLVEKMRSS
                     PTIPYTLANAFESFFNAEGLGNEEERLALAAKVLQAFARGRLRTSRRLSIAPTGSISM
                     LIDASAGIEPNFAWSWSRRIAKADGDGQETREFHHKLLSPLQAAELHDTGALSDPVFV
                     TAYDITPEEHVEVTGIFAGVVDSGISKTVNLPNDATVDQVKAIYRRCYEMGAKGITIY
                     RDGSRSFQPIETTKKPEKAEQPEEKQPLSSRVKERPGLVVFGKTIKETTPWGSIYVTL
                     NFDGNDPFEVFATIGKSGSELKAMTEALSRAISIGLRSGGKLEDFIATLKGLSGKEYW
                     LFEFDDKHVARSIPDAIAVLLERLIGKDIGTQGVSAGVECPECGAPMEMISGCEYCFS
                     CGYSPCK"
     gene            3457..>3932
                     /locus_tag="P5201_04475"
     CDS             3457..>3932
                     /locus_tag="P5201_04475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013047533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HRV97820.1"
                     /translation="MTRYDFDTVIDRRNTDCAKWDAMKPLFGTNDLLPMWVADMDFKA
                     PPEVIEALRERVEHGIFGYTRRFEPYREAIAGWFLRRHGWEIRTEWIRHAPGVVPALA
                     AAIMAFTQPGEGILVQPPVYYPFFSTIKGRGRTVVENPLKLVNRRFEMDFDDLEKKL"
BASE COUNT          858 a         1160 c         1081 g          833 t
ORIGIN      
        1 aaaagcatgt ctcctgcttt ttccgttcct tctgtcgagg ttatgggatg ggcgagaaag
       61 aattaccttg aaccatccta ccctagaaaa cgatacgacg agcagcggac gttctccgac
      121 ggacgtttcc gtgatcgtgt gttgcgaggc gacaagtcgc ttgaacgcgc gctcgactcc
      181 ttccaggagc atgcggccct ccacagcaga tacctccagt tcaacataga ggaagaagcc
      241 gaccttgtgc aggtgatcgt gaaagaaagc aacgacgagg agaagatcat ccgcaaaatc
      301 ccttccgacg aaatagtgcg cctcatagag cggcggaacg agattctcgg ttttctcttc
      361 gatatcgtcg cttaaaaacc tcccccttca taacaagacg atgaggccgg aagagctctg
      421 cgctccttcc ggcctcatct tcttatccgg cgatcattac ttgcaggggg agtacccgca
      481 gctgaagcag tactcgcacc cggaaatcat ctccatcggc gcgccgcact cagggcactc
      541 gacgcctgcg cttacgccct gcgttccaat atcctttcct atcagccgtt cgagcagcac
      601 tgcgatcgcg tccggtatgg aacgggcgac gtgcttgtcg tcgaactcga agagccagta
      661 ctccttccct gaaagtccct taagcgtggc gatgaagtcc tccagcttgc cgccgctgcg
      721 aaggccgatg gaaatcgcgc gagagagcgc ctccgtcatg gctttcagct cagagccgct
      781 cttgccgatg gtggcgaaca cctcgaacgg atcgttgccg tcgaagttca gggtgacgta
      841 gatactcccc cagggcgtcg tctccttgat cgtctttccg aagacaacca atcccgggcg
      901 ctcttttacc ctgctggaga gcggctgctt ctcctccggt tgctcggcct tctccggctt
      961 tttcgtcgtc tcgatcggct ggaaggagcg ggatccgtcg cggtagatcg ttatcccctt
     1021 cgcgcccatc tcgtagcagc gccggtagat cgccttcacc tggtcgacgg tcgcgtcgtt
     1081 ggggaggttc acggtcttgc tgatgccgct gtccacaacg ccagcgaaga taccggtcac
     1141 ctcgacatgc tcttccgggg tgatgtcgta ggcggtcacg aagacggggt ccgagagcgc
     1201 ccccgtgtcg tgcagctccg cagcctgaag gggcgagagc agcttgtgat ggaactctct
     1261 cgtctcctga ccgtcgccgt cggctttggc gatacggcgg ctccagctcc aggcaaagtt
     1321 cggttcgata ccagcgctgg cgtcgatgag catggagatc gagccggtgg gagcgatgga
     1381 gagacgtctg cttgtgcgga ggcgtcctct cgcgaacgcc tgaaggacct tcgcggcaag
     1441 cgcaaggcgc tcttcttcat tgccgagccc ctcggcgttg aagaaggact cgaacgcatt
     1501 cgcaagggtg tagggaatcg tcggcgacga acgcatcttc tcaacaagcg actgcattga
     1561 ttcacgatca agggaagcaa accacgattc ggggaagagc tcctccccct gttcagctgc
     1621 aaatctgcgg aagagctcct cgacaaggtg cgcgtcgggg aacggctcct tccccatctc
     1681 ctcggccatc ccgacgcttg cgcagagcgc cgcgctcccc atggaatcgg cgagcgtcct
     1741 gcagaaaacc ttgaaacgct ccgagccgta ccgcatcctc agcaagatgg cggcgtcggc
     1801 cagtcccatg atacccagtc ccacgggacg gattcgccgc gtgcgctccc cgatcttctc
     1861 cagcgggtag gagcaggcgt cgataacgag gtcgagatag tacatcgacc ggaaaacctg
     1921 atctgaaaaa gcggcgaagt cgaaacgaac gttcccgtcc tccctgcgct ccacgaaggc
     1981 ctctacattc actgagccaa gattacagct ggtataattt ggcagcggtt gttcgccgca
     2041 tggattcgta gactcgatac gccacgtttc gtcgagcttc agcagattgt cggcgttcgc
     2101 cctatcgatg aaaaagatgc ctggatcgcc gcgcttccac gcgttttcgc agagtttgct
     2161 ccacagttcg gcgccgttga gcgagcggac aacactcccg ttgcttcggg aaacgaggtt
     2221 gaagtcggcg ccggtctgcg cggacttcat cagtttatcg gagacgcaga cggagatgtt
     2281 gaagtacgga agcttcccct cgtcaacctt cgcgtcgatg aagtcgaaga tgtcggggtg
     2341 gtcgtcgaag agcatcccca tgagcgcggc gcgcctgcgg cctccctgca ccaccaccgc
     2401 gcccattgtg ttccacgttt ccatgaacga caccggaccg ctcgcctggc cgccgatgcc
     2461 tccgttgatg acggagctgc gctcgcgaag atggccgaag tttcctccga cgccgccgcc
     2521 gtacttgctg atcacggcgg catccttgac ggagtcgaaa atgccgtcga tactatcggg
     2581 gacctctatg acaaagcagg cgaagagctg ctgccccttc gtcttcgaac gatggagcaa
     2641 agagtagtct tcaaacgtca gttcctcagg atcgcaatag atcctctccg ccatcccggg
     2701 gtccacggtc aaacccgccc ccgctccgaa gagacacggg gagttgaaca ggaagcggcg
     2761 gttcaggata tcgtcgaaga tattccgttc cagcctgcga agccactgca gatcctcttc
     2821 ccctcctgcg agatacgacg tctccgcgca tgcgacagta cgcgctaccc gacgggcaaa
     2881 ctcctcaaac gatttctcct tccggattcc acgtagaacc gggtcatacc ggcgggcaaa
     2941 atagcgctgg tcgagaagcc acagagcatt ctcggagaga cgttcaccgc ctgccggcgc
     3001 agaggatgaa aaggaagaac gaaagcgttc cttgtaggaa actgacacgt aaacccctcc
     3061 taaaaaacac tatatacaca atgacaaaag tttcacacac aagatgttga gtgaaattgc
     3121 gattggacta tcagactacc acttcttcca ctttcctaca agaggggacg cgtgacttca
     3181 gaattggaag aacatggcgc ctaagccctc tctttgtaga tgcagtttgc acacattttg
     3241 ttatgagttt tccatcaagg ttatgatttc gttccaaata aagacgccgt gagactattt
     3301 ccctgggcgt aaatcgtaaa gctttgcatt tttagtataa ctctggaatt gttcgctttt
     3361 gggggacgga aaagcagttt ttctcaatgc agcatcttct cctcgtttaa aaaaatgata
     3421 tgatagtagc aacttgttga aagcgaggtt cgcattatga cgcgctacga cttcgatacg
     3481 gtgatcgaca gaagaaatac tgactgcgcc aagtgggacg ccatgaaacc gctcttcggg
     3541 acaaacgatc tgcttccgat gtgggtggcg gatatggatt ttaaagcgcc ccccgaggta
     3601 atcgaagccc tccgggagcg tgtagaacac ggtattttcg gctacacccg ccgttttgaa
     3661 ccgtatcgcg aggcgatcgc aggatggttc cttcgcaggc acggctggga aatacgcacc
     3721 gagtggattc gccacgcccc cggagtcgtt ccggcgctcg cggccgccat aatggccttt
     3781 acacaacccg gggaaggaat actggtgcag cctcccgtct actacccctt cttctccacc
     3841 atcaagggaa gggggcgcac cgtcgtagag aaccccctga agctggtcaa ccgccgtttt
     3901 gagatggact tcgacgacct tgaaaagaag ct
//
LOCUS       DAOTIL010000158         3893 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminobacteriaceae bacterium isolate AMR_MDS_3026
            NODE_27641_length_3893_cov_3.381449, whole genome shotgun sequence.
ACCESSION   DAOTIL010000158 DAOTIL010000000
VERSION     DAOTIL010000158.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868050
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminobacteriaceae bacterium (wastewater metagenome)
  ORGANISM  Aminobacteriaceae bacterium
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae.
REFERENCE   1  (bases 1 to 3893)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3893)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:19:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,224
            CDSs (total)                      :: 3,198
            Genes (coding)                    :: 3,184
            CDSs (with protein)               :: 3,184
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3893
                     /organism="Aminobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_27641_length_3893_cov_3.381449"
                     /isolate="AMR_MDS_3026"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:3034609"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1244
                     /locus_tag="P5201_04480"
     CDS             <1..1244
                     /locus_tag="P5201_04480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006301071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HRV97821.1"
                     /translation="MQLSGKRLLIVGLGRIGTQVALRCRAFGMEIMAFDPYVSKEKAD
                     RAGVQLVDDLRGGMALADVITLHVPLTRESRGMLDAATLRACKRGAYLVNCARGGLVD
                     EEACAEAVRSGILAGAAFDVFDGEPVRADHPLLASDIRGKVILTPHIGANTEEAQSAV
                     AVIAASNLAAALLGKPYEHAVNLPFSEQLLSDGSRAYLSLARKMGYLAANLLREPVRE
                     IKVALRGPLFTCGDEPVCFEVPYHYSPFTVAGLKGMLEFSHGPEVNYMSAPLLAEDKG
                     IAVEETRADGGTYRNLLELSITSSGGRETVTVCGTVTEEGRQRVVKLDGYPIEFVPEG
                     VVLLFSNHDRPGVIGKVGTLLGEAGSNIANFALGRKNGSGLAVAALQIDNDVPDKVVE
                     TFKADADLLWAVKVDFSEAAR"
     gene            1241..2011
                     /locus_tag="P5201_04485"
     CDS             1241..2011
                     /locus_tag="P5201_04485"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006301070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HRV97822.1"
                     /translation="MSCGHDHEKEEINGMKPENSGERSPEVKEAAGTSPLRPTGAAGK
                     RIFLVRHGETSWNREGRFQGQIDVPLNDAGLRQADAVANTLKDVPFDRIISSNLSRAL
                     RTALPLASRMRMGMGVETDDDLQEIGHGLWEGRTAAEVETMYPGALALWHTRPETVVM
                     PEGESLADVQRRAWPALLRIAEGKGETVAVFTHDAVLKVLLMKALDCPLSSFWRFQLA
                     NGSITLLELGNKGWRIPLMGEAGHLGSVFGKRAEQKGL"
     gene            complement(2127..2789)
                     /locus_tag="P5201_04490"
     CDS             complement(2127..2789)
                     /locus_tag="P5201_04490"
                     /inference="COORDINATES: protein motif:HMM:NF016921.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MT-A70 family methyltransferase"
                     /protein_id="HRV97823.1"
                     /translation="MFNQVDDLLEYAQGKTYRTILADPPWQFSNRTGKMAPEHKRLNR
                     YPTMPLGEICSLPVKEIAADTAHLYLWVPNALLPDGLTVLSAWGFEYKTNLIWYKTRK
                     DGGPDRRGVGFYFRNVTEMILFGVKGKNARTLQPGRSQENIITAQKREHSRKPDEQYR
                     LIESCSEGPYLELFARGPRPGWDVWGNQSEEYAPDWATYANHSQAKRDNPQQLTLIET
                     QA"
     gene            complement(2782..3378)
                     /locus_tag="P5201_04495"
     CDS             complement(2782..3378)
                     /locus_tag="P5201_04495"
                     /inference="COORDINATES: protein motif:HMM:NF020757.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009036 - type II site-specific
                     deoxyribonuclease activity [Evidence IEA];
                     GO_process: GO:0009307 - DNA restriction-modification
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BglII/BstYI family type II restriction
                     endonuclease"
                     /protein_id="HRV97824.1"
                     /translation="MPIELIPLNIRENYEVHEWKHAVAILSQDFPDEWTDILAVLESF
                     RLKKSWITDPGGRKSKVSEHIDSFLYRRGWVEKEFQTKVVVDDLSMSSPTHKVDCFKN
                     KVALEIEWNNKDPFYDRDLNNFRLLFDLRAISAGVIITRCDDLQDIFNDLGRSPSYGA
                     STTHMRKLLPKIEGGSGGGCPVLVFGITKHLYEEDDHV"
     gene            complement(3526..>3893)
                     /locus_tag="P5201_04500"
     CDS             complement(3526..>3893)
                     /locus_tag="P5201_04500"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRV97825.1"
                     /translation="LRAGTQRTEAVCATVKGDFESLAHRIGSFANVVNGLSSLPSILE
                     QMEDRLLKRMEVFEEILRRTPEKKPGKSAQPLPEEPFSREVLEKLTLPVLKEIMKKKG
                     IPGKGLKKKAEFIDAILGG"
BASE COUNT          700 a         1256 c         1158 g          779 t
ORIGIN      
        1 ggatgcagct ctcggggaag cgcctgctga tcgtcgggct ggggcgcatc ggaactcagg
       61 tcgccctccg ctgccgcgcc ttcggcatgg agatcatggc cttcgacccc tacgtctcga
      121 aggagaaggc cgaccgcgcg ggggtgcagc tcgtcgacga cctcagggga ggaatggccc
      181 tcgccgacgt gatcacgctc cacgtgccgc tgaccaggga gtcgcggggg atgctggacg
      241 ccgcgacgct ccgcgcctgc aagcgggggg cctacctcgt gaactgcgcg cgcggcggcc
      301 tggtcgacga ggaggcctgc gccgaggctg tccgttcggg aatcctcgcc ggagccgcgt
      361 tcgacgtctt cgacggcgag cccgtcagag ccgaccaccc gctgctggcg tcggacatcc
      421 gggggaaggt gatcctcacg ccgcacatcg gcgcgaacac cgaggaggcc cagtccgccg
      481 tcgccgtgat cgccgcgtcg aacctcgccg ccgcgctgct cgggaagccg tacgaacacg
      541 ccgtcaacct ccccttcagc gaacagctcc tctccgacgg cagccgcgcc tacctctccc
      601 tcgcccgcaa gatggggtac ctcgccgcga acctcctgcg cgagcccgtg cgcgagatca
      661 aagtcgccct ccgcggaccg ctcttcacct gcggcgacga acccgtctgc ttcgaagtcc
      721 cctaccacta ctcccccttc accgtcgccg gactcaaggg gatgctggag ttctcgcacg
      781 gccccgaggt gaactacatg tccgcgcccc ttcttgccga ggacaagggc atcgccgtcg
      841 aggagacccg cgccgacggc ggtacctacc gcaacctgct ggagctctcc atcacttcct
      901 ccggagggcg cgagaccgtc accgtctgcg gcaccgtcac cgaagagggg cggcagcgcg
      961 tcgtcaagct cgacggctac ccgatcgagt tcgtccccga aggagtggtg ctgctcttca
     1021 gcaaccacga ccgccccggc gtcatcggca aggtcggcac cctgctcggc gaggccggat
     1081 cgaacatcgc gaacttcgcc ctcggccgga agaacggaag cggcctcgcc gtcgccgcgc
     1141 tccagatcga caacgacgtc cccgacaaag tcgtcgagac gttcaaggcc gacgccgacc
     1201 tgctctgggc cgtcaaggtc gacttttcgg aggcggcgcg atgagctgcg ggcatgatca
     1261 tgagaaggag gagataaacg gtatgaagcc ggaaaattca ggggagcgtt ccccggaggt
     1321 gaaagaggct gcggggacga gtcccctgcg accaacggga gctgcgggga agcgcatctt
     1381 cctcgtccgg cacggcgaga cgtcgtggaa ccgcgaaggg cgcttccagg ggcagatcga
     1441 cgtcccgctc aacgacgccg gactgcgcca ggccgacgcc gtcgccaaca ccctgaaaga
     1501 cgtcccattc gaccggataa tctccagcaa cctctcccgc gcgctccgca ccgccctccc
     1561 cctcgcctcc cgcatgcgca tggggatggg cgtcgaaacc gacgacgacc ttcaagagat
     1621 cggccacggc ctctgggagg gacgcaccgc cgccgaagtg gagacgatgt accccggagc
     1681 cctcgccctc tggcacaccc gccccgagac cgtcgtcatg cccgaaggcg agagcctcgc
     1741 cgatgtgcaa cgccgagcct ggcccgccct gctgcgcatt gccgagggaa agggcgaaac
     1801 cgtcgccgtc ttcacccacg acgccgtgct caaagtcctg ctgatgaagg cgctcgactg
     1861 ccccctctcc agcttctggc gctttcagct cgctaacggc agcatcaccc tgctggagct
     1921 gggaaacaag gggtggcgca tcccgctgat gggcgaggcg gggcacttgg gaagcgtctt
     1981 cgggaagagg gcggagcaga aggggttgta acaaatcaga ccgatgaacg tcttttccga
     2041 cagtaggctc caccggaccc aaaccttcgc ctcgaatatg agcaaaactc cctgctgcgt
     2101 ctccgaggcg aaggttcagt attgcatcac gcctgtgttt cgatcaaggt gagctgctgc
     2161 gggttgtctc gcttggcctg ggagtggttc gcgtaggtcg cccagtcggg ggcgtactcc
     2221 tctgactggt ttccccacac gtcccatccg gggcgcggac cgcgcgcgaa gagttccaga
     2281 tacggccctt cgctgcacga ttcgatcagg cggtactgct cgtccggctt tctgctgtgc
     2341 tctcttttct gcgccgtgat gatgttctcc tgagagcgtc ccggctggag ggtccgggcg
     2401 ttcttgccct tcactccgaa gaggatcatc tcggtgacgt tccggaagta aaagccgact
     2461 ccgcggcggt cggggcctcc gtccttccgc gttttgtacc atatcagatt cgtcttgtat
     2521 tcgaagcccc atgccgaaag caccgtcagc ccgtcgggaa ggagcgcgtt gggaacccat
     2581 aagtagagat gcgccgtatc ggcggcgatc tctttcaccg gaaggctgca aatctcgccg
     2641 agcggcatcg tcggatagcg gttgagccgt ttatgttcgg gcgccatctt gccggtgcgg
     2701 ttcgagaact gccagggagg atcggccagg attgtcctgt atgtcttccc ctgcgcatat
     2761 tccagcagat cgtctacttg attaaacatg atcgtcttcc tcgtagaggt gtttggttat
     2821 gccgaacacc agcacaggac atccgccgcc gcttccgcct tcgattttgg gtagcagctt
     2881 tctcatgtgg gtggtcgacg cgccgtagga ggggcttcgt ccgagatcgt tgaaaatatc
     2941 ctgcaggtcg tcgcatcggg tgatgataac tcccgcgctg attgcccgca ggtcgaaaag
     3001 cagccggaaa ttgttcaaat cacggtcgta gaaggggtct ttgttgttcc attcgatctc
     3061 cagtgccact ttgtttttga aacagtcgac tttatgcgtg ggcgacgaca tcgacaagtc
     3121 gtccacgacc actttggtct ggaactcctt ttcaacccag ccgcgcctgt agagaaagct
     3181 gtcgatgtgt tcagacacct tcgattttcg cccgcccgga tccgtaatcc atgatttttt
     3241 caaacgaaag gattccagga cggccagaat atccgtccac tcgtcgggga aatcctgcga
     3301 aaggatggcg acggcgtgct tccactcgtg aacttcgtaa ttttctctga tgttgagcgg
     3361 tatcaactct atcggcatgt tttcacctct tccgcatcgt accatatttt cacgaatttg
     3421 tggcgcctgt cgtgggggaa atacgtccgt tgcgctctgc gtacaatcag cctttttctc
     3481 tcaatcgacg tttttcctaa aattttcttc ggtggacgtc ctgcatcacc ctccgagaat
     3541 cgcgtcgatg aactctgctt ttttcttcag ccccttgccg ggaattccct tcttcttcat
     3601 gatctctttg agtacgggaa gagtgagctt ttcaagaact tcacggctaa agggctcttc
     3661 aggcagaggc tgcgcgcttt tccccggttt cttttccggc gttcggcgca ggatttcctc
     3721 aaaaacctcc atcctcttca acaagcgatc ctccatctgt tccagtatcg aagggagcga
     3781 agacagtccg ttcactacgt tggcgaagga gccgatccgg tgcgcaaggc tctcgaagtc
     3841 ccctttgacg gtggcgcaga cggcttccgt tcgctgcgtc ccggcgcgga gcg
//
LOCUS       DAOTIL010000159         3888 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminobacteriaceae bacterium isolate AMR_MDS_3026
            NODE_27677_length_3888_cov_3.919384, whole genome shotgun sequence.
ACCESSION   DAOTIL010000159 DAOTIL010000000
VERSION     DAOTIL010000159.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868050
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminobacteriaceae bacterium (wastewater metagenome)
  ORGANISM  Aminobacteriaceae bacterium
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae.
REFERENCE   1  (bases 1 to 3888)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3888)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:19:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,224
            CDSs (total)                      :: 3,198
            Genes (coding)                    :: 3,184
            CDSs (with protein)               :: 3,184
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3888
                     /organism="Aminobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_27677_length_3888_cov_3.919384"
                     /isolate="AMR_MDS_3026"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:3034609"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..983)
                     /locus_tag="P5201_04505"
     CDS             complement(<1..983)
                     /locus_tag="P5201_04505"
                     /inference="COORDINATES: protein motif:HMM:NF012722.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing protein"
                     /protein_id="HRV97826.1"
                     /translation="MRGYTLKLFWWFWLTAAATGLAHHLTSVITEGNIFREQMIANVQ
                     EIALVYGERVASKEAREGPEEAVRYLERLRRTHRFFGFLFGEDGTELTGQPKAPESIG
                     KIVDEARNTGAPVVRMERGRPLFAVPLEEFPGLVFAGRILNPFFESRSLPVLRLIAAA
                     LVAGLLSWFLAVRLAKPVRKLRGAVQRLAAGELGARAEHTAKRNDEIGQLAKDFNRMA
                     DRIEELLRGQRQLLADVSHELRSPLTRLGVGLELLRRRSASPDDEAFKRIETELGRID
                     NLIGSLLKLGRLEALEQPEKRTRIDLTALLRQICADAAFEAAPLGKRIETD"
     gene            complement(965..1657)
                     /locus_tag="P5201_04510"
     CDS             complement(965..1657)
                     /locus_tag="P5201_04510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005660363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HRV97827.1"
                     /translation="MHRILLLDDDIELCDLLTEYLRPEGFEILVAHDGEEGLAAALSS
                     PVDLVLLDVMLPGKNGFDVLRELRAHSPVPVLMLTAKGDEIDRIVGLELGADDYLPKP
                     FNPRELLARIRAVLRRGERPETALSERLSAGDIEMDLGARTVRVAGKDTELTSVEFKL
                     LEALLRSAGKVVSREKLAMAALERTLGPFERSLDVHVSNLRRKLGPADDGSSRIRTLR
                     SEGYLLACAATH"
     gene            1887..2756
                     /locus_tag="P5201_04515"
     CDS             1887..2756
                     /locus_tag="P5201_04515"
                     /inference="COORDINATES: protein motif:HMM:NF019430.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Spy/CpxP family protein refolding chaperone"
                     /protein_id="HRV97828.1"
                     /translation="MKKMKKRILVGSLLFVLVVLLVGGVSQYAFASRMMGAGPWGGMS
                     RGHDRGDSAFKAELLRALNPMDFTEEQWKKFDEILGGLRDQGKDFRRGMMKEMWNTRK
                     EIHAMMTADGFDEEKAKEMIAAVRPSFVSMAQEVLKARNELYGILNDAQRNKVGAVLD
                     VIGKRLEQPFSGPRELHAAMTRRLELTPDQKGKVEAITKETAPKMHAFAKTLFDQVKS
                     EHAALANGPMSDEQLAESAGKIADLAEGALMEMGRVRAQVWETLTPEQREEMGKMRHP
                     MGGMERRPGRMRK"
     gene            3082..>3888
                     /locus_tag="P5201_04520"
     CDS             3082..>3888
                     /locus_tag="P5201_04520"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HRV97829.1"
                     /translation="MTSSDFEYGNDPQLRESIERSILLGNLRRMQRLLWGLALFLALV
                     LLCSPFLYEKSVQHFGSAAPLEVQILWRSAWLAFDLFALFGIRRILSHPPERIDLFHR
                     AVETGVPLINMLFVVSLLAAGYAYNPSLEILYIALFAMAPLIRLNAAKTFIVVGMPLG
                     LFVALMDRRAAGVAHMSNMISLTAVVILAAIVFREMYRSTVREMEQQAIIERQLKIME
                     TFANTDGLTGLANRRSLDENLETEWRRGMREHNFLAVVMLDIDLFKSYNDQ"
BASE COUNT          784 a         1132 c         1190 g          782 t
ORIGIN      
        1 aagtccgttt cgatcctctt ccctagggga gccgcttcaa aagccgcgtc cgcacaaatc
       61 tgccgcaaaa gcgccgtcag gtcgatgcgc gtcctcttct ccggctgttc gagcgcttcg
      121 aggcgcccca gcttcagcag ggagccgatg aggttgtcga tccgccccag ctccgtctca
      181 atgcgtttaa aggcctcgtc gtccggcgac gcggaacggc ggcgcaggag ttcgagtccc
      241 acgccgagcc gcgtcaacgg cgatcgaagc tcgtgcgaga cgtcggcaag aagctgacgc
      301 tgcccgcgaa gcagctcttc tatcctgtcc gccatacggt tgaagtcctt cgccagctgg
      361 ccgatctcgt cgttccgctt ggctgtatgc tccgcccgcg cgccgagctc cccggcggcg
      421 aggcgctgca ccgcgcctcg gagcttcctg accggtttgg cgaggcggac ggctaaaaac
      481 cacgagagca ggccggcgac cagcgcggcc gcaatgaggc gcagcaccgg gagacttctg
      541 ctttcaaaga aagggttgag aatgcgtcct gcgaagacga gtcccggaaa ctcctccagc
      601 gggacggcaa aaagaggacg cccccgttcc atgcgtacaa cgggcgcccc tgtgtttcgc
      661 gcttcgtcga cgatctttcc gatcgattcc ggcgccttcg gctgtcccgt cagctccgta
      721 ccgtcttcgc cgaacagaaa cccgaaaaaa cggtgagtac gacggagccg ctccaggtag
      781 cggaccgctt cttccgggcc ttcccgcgct tctttcgatg caacgcgctc tccgtagacg
      841 agcgcgatct cctgtacgtt cgcgatcatc tgctcgcgga agatatttcc ctccgtgatc
      901 acactggtca ggtggtgcgc cagtcccgtc gccgccgcgg tcagccagaa ccaccagaag
      961 agctttaatg tgtagccgcg catgcaagaa ggtacccttc gcttcggagc gtccgtatcc
     1021 tggacgaacc gtcgtccgcc ggtccgagtt ttcggcgcag gttgctgacg tggacgtcca
     1081 gacttctctc gaacggtccc agcgttcgtt caagcgcggc catcgccagt ttttcccgcg
     1141 agacgacctt cccggcggag cgcagcaagg cctccagcag cttgaactcc actgacgtca
     1201 gctccgtgtc tttccctgcg acgcgtacgg ttctcgcgcc gaggtccatc tcgatatccc
     1261 ctgcgctcag acgttcggaa agcgctgttt ccggccgctc ccccctcctg aggacagcgc
     1321 gaatccgagc gagcagctcg cgcggattga acggcttcgg gaggtagtcg tcggcgccca
     1381 gctccagccc gacgattctg tcgatctcgt cgcccttggc ggtgagcatg agcacgggaa
     1441 cgggagagtg tgcgcgcagc tcgcggagca cgtcgaaccc gtttttcccc ggcagcatga
     1501 cgtcgagaag caccaggtcg accggcgacg aaagcgcggc ggcaagcccc tcctcgccgt
     1561 cgtgtgctac gagtatttca aacccctccg ggcgcaggta ttcagtcagc aggtcgcaga
     1621 gttcgatgtc gtcgtcgagc agcagtattc gatgcacgtt cctttcctcc ccgggtgtgc
     1681 gaagtccgcc tcttcggcgg ctctctatcc gttcattata cgacgacagg ctttggaggg
     1741 gtgtaaggaa ttgaaaagat gggtatcaag agctttacgt ttcttgacct ttatccacaa
     1801 agcgcgaaga gcgcattgag agatcgagga gtacactctt cgcgtcgggc gaagaatgcc
     1861 gaaaactcaa cgggaggcga aacacgatga aaaagatgaa aaaaagaatt ttagtagggt
     1921 ctcttctatt tgtactggtt gtactgcttg tcggcggcgt ttcacagtat gcgttcgcaa
     1981 gcagaatgat gggcgccggg ccttggggag ggatgtcccg cgggcatgac aggggagatt
     2041 ccgcgttcaa agcggaactt ctccgggcgc tgaatccgat ggatttcacc gaggagcagt
     2101 ggaagaagtt cgacgagata ctcggcgggc tccgtgatca gggaaaggac ttccgtcggg
     2161 gcatgatgaa ggagatgtgg aacactcgca aagagattca cgcgatgatg accgcggatg
     2221 gcttcgacga agagaaggcg aaggaaatga tcgctgcggt ccgcccgtcg ttcgtgtcga
     2281 tggcccaaga agtgttaaag gcacggaacg agctctatgg catattgaac gacgcgcagc
     2341 ggaacaaggt gggcgctgtt ctcgacgtca tcgggaaacg gttggagcag cctttcagcg
     2401 ggcctcggga actgcatgcc gcaatgacgc gccgccttga actgacgccc gaccagaaag
     2461 gaaaggtcga ggcaattacg aaggagaccg cgcccaagat gcacgccttc gcgaagacgc
     2521 tgttcgacca ggtgaagtcg gagcatgcgg cgcttgcgaa cggaccgatg agcgacgagc
     2581 agctcgcgga atcggccgga aaaatcgccg atcttgccga aggagctctg atggagatgg
     2641 gcagagtgag ggcgcaggtg tgggagacgt tgactcccga gcagcgcgaa gagatgggga
     2701 agatgcgcca tcccatgggc ggaatggagc gcaggccggg gaggatgcgg aagtagcagc
     2761 ttcgcggaac gaagtgctgc gcgcggcgcg ggatcttgat ccgcgccgcg cacagactga
     2821 aaaaacgacg cgcgagaagc gttccgttcg ttttttgaaa ggcgttcgag ggagaggttc
     2881 ctcggacgct tttgcatatt tccctcgtct ccggcggcgc ctctacctga gtatttggga
     2941 gatattcctt tgtttgacag cgcacacctc caaagataca ctgtgctaac gaagatattt
     3001 caagcggggg cgcgttatga aaaaaaactg tgaacatatc gggagcgccg cggcgttccc
     3061 tccgtgggga ggagcgatac gatgacctcc tccgacttcg aatacgggaa cgatccgcaa
     3121 ctccgggagt cgatcgagcg cagcattctc ctgggcaatc tgcgaaggat gcagcgcctt
     3181 ctttgggggc ttgcgctctt tctggcgctg gtgctcctct gcagtccttt tctgtacgaa
     3241 aagtcggtgc agcacttcgg atccgcagct ccccttgaag tgcagattct ctggcgcagc
     3301 gcctggcttg cttttgacct cttcgcgctt ttcggtatcc gccgcattct ttcgcatccg
     3361 ccggagcgga tcgacctttt ccacagggcg gtggagacgg gcgttccgct catcaacatg
     3421 ctcttcgtcg tttctctgct ggcagcaggg tatgcgtaca acccctcttt ggaaattctg
     3481 tatatcgccc ttttcgcaat ggcgcctctc atacggttga atgcagccaa aacattcatc
     3541 gtcgtcggga tgccgctggg gctgtttgtg gcgctcatgg accgccgcgc ggccggagtc
     3601 gcgcatatgt ccaacatgat cagcctgacc gccgtcgtca ttctcgcggc gatcgtcttc
     3661 agagagatgt accgttccac cgtccgcgaa atggagcagc aggcgatcat cgagcgtcag
     3721 ttgaaaatta tggaaacgtt tgcaaacacg gacggactga ccgggctcgc caatcgcagg
     3781 agtctcgacg agaatctgga gaccgaatgg agacgaggta tgcgagagca taacttcctg
     3841 gccgtcgtca tgctggacat cgatcttttc aagagctaca acgatcag
//
LOCUS       DAOTIL010000160         3886 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminobacteriaceae bacterium isolate AMR_MDS_3026
            NODE_27695_length_3886_cov_4.203080, whole genome shotgun sequence.
ACCESSION   DAOTIL010000160 DAOTIL010000000
VERSION     DAOTIL010000160.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868050
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminobacteriaceae bacterium (wastewater metagenome)
  ORGANISM  Aminobacteriaceae bacterium
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae.
REFERENCE   1  (bases 1 to 3886)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3886)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:19:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,224
            CDSs (total)                      :: 3,198
            Genes (coding)                    :: 3,184
            CDSs (with protein)               :: 3,184
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3886
                     /organism="Aminobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_27695_length_3886_cov_4.203080"
                     /isolate="AMR_MDS_3026"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:3034609"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..860)
                     /locus_tag="P5201_04525"
     CDS             complement(<1..860)
                     /locus_tag="P5201_04525"
                     /inference="COORDINATES: protein motif:HMM:NF012245.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HRV97830.1"
                     /translation="MNNRMKNGNPAPVLDDVVLGAVESLSQQLQQMFWVGKNQEKAAE
                     EMRTSASSTRSDMERYALGVSKIIEGLSHLSESCIAMGDIAMASKEIESETREGVLNA
                     VTGMERLEKTLDALSTLSEEHARMTDELLHSTADVGIFLERIDRIAKQTRLLALNASI
                     EAARAGEAGRGFNVVAQEVGKLAQSTQETAASISKAIETMGDRIRHVVDGLKETESAL
                     SAGREETQDALARARQAGEGVMRFSGSFSELIKVLEEERTSITEAVAETQSLQEGTKA
                     VLSDVGTLGR"
     gene            complement(950..1564)
                     /locus_tag="P5201_04530"
     CDS             complement(950..1564)
                     /locus_tag="P5201_04530"
                     /inference="COORDINATES: protein motif:HMM:NF013933.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysE family transporter"
                     /protein_id="HRV97831.1"
                     /translation="MDLSILFRGVVIGFSIAAPVGPIGVLCIRRTLTEGRASGLVSGL
                     GAASADAVYGCIAGFGLTFLSNFLVSRQTELRLAGGLFLLYLAVKTFFAQPAQKAAEV
                     RGHGLLGAYLSTLFLTLTNPMTIISFAAIFAGLGLAEGSGNYISAGVLVLGVFAGSAL
                     WWLLLSGGFGLFREKFTASGLRWLNRLSGVIIGVFGLLALWSVF"
     gene            complement(1696..2913)
                     /locus_tag="P5201_04535"
     CDS             complement(1696..2913)
                     /locus_tag="P5201_04535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013049185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SLC13 family permease"
                     /protein_id="HRV97832.1"
                     /translation="MVNGFFVFFVFAAVYLGMIFGRFPRLALDRTGIVLLGAIVLLAA
                     GKASLEGGRSGIDVPTILLLFGFMVISAQFRLGGFYSEVSRRIGALAAPPSLIMGAVI
                     GASALMSALLTNDVVCLAMTPVLGAGCIRRGLNPLPFFLALACAANIGSALTLIGNPQ
                     NMLIGQYLSLPFGGYLLFAAFPVFLSLLSLWGIMLFLFRGRWTLQMDEQLVDERSFDA
                     WQTLKGGVVLTVVVGLFLFSTLPRDLTALGAAGVLLLSRKMASRRTLEIVDWQLLLLF
                     LGLFVVNAAFESAGGLQILKEFLESAGVSLSSPAWLYGVTTVLSNLVSNVPAVMLLLP
                     LVRDVPQAGYILAVSSTFAGNLLIVGSIANIIVAGEAERMGIPFDWKQHALVGVPVTL
                     ISLAAGLACLWLF"
     gene            3380..3586
                     /locus_tag="P5201_04540"
     CDS             3380..3586
                     /locus_tag="P5201_04540"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRV97833.1"
                     /translation="MAGKYGFTAATETEREVFRRNDRHLDDMFSVSWRGPFEGFSASR
                     KADLAEPSFSQTEMRWRGPFEGLR"
     gene            complement(3647..>3886)
                     /locus_tag="P5201_04545"
     CDS             complement(3647..>3886)
                     /locus_tag="P5201_04545"
                     /EC_number="3.2.2.20"
                     /inference="COORDINATES: protein motif:HMM:NF015319.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008725 - DNA-3-methyladenine glycosylase
                     activity [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase I"
                     /protein_id="HRV97834.1"
                     /translation="KGSFSAYLWDFLDGEPIVNRWKTMEEVPAVTPLAEKVSGCMKKE
                     GFVFFGPVICYAHMQATGMVNDHLTSCFRHPESGY"
BASE COUNT          966 a         1131 c         1040 g          749 t
ORIGIN      
        1 cttctgccga gagtgcctac atcggaaagg actgccttcg tcccttcttg cagagattgt
       61 gtttccgcta cagcctccgt gatggaggta cgttcctctt cgagaacctt gatcagctcg
      121 gagaacgaac cgctgaaacg catgacgcct tcgcccgcct gacgagcgcg cgccaaggcg
      181 tcctgcgttt cttcccttcc ggcggagagc gcgctctccg tttctttcag gccgtcgacg
      241 acatgccgga tgcggtctcc catcgtttcg atagcttttg agatggacgc cgccgtctcc
      301 tgcgtggact gcgccaattt ccccacctct tgggcgacga cgttgaaacc gcgccccgcc
      361 tccccggccc ttgcagcttc aatggacgca ttgagcgcga ggagccgcgt ctgtttcgca
      421 atacggtcga ttcgctccag aaagattccg acatccgcag tcgagtgcaa aagctcatcg
      481 gtcatgcgcg catgctcctc agagagagtg gaaagagcgt ccaacgtttt ctccaggcgc
      541 tccatacccg tcacagcatt gagcactccc tcgcgcgtct cggactcgat ctctttcgag
      601 gccatggcaa tatcgcccat tgcaatacag gattctgaaa gatgcgaaag cccttcgatg
      661 atcttggaga cccccaaggc gtatcgttcc atgtccgacc gggtagagga cgcgctcgtc
      721 cgcatctctt ccgctgcttt ttcctggttt ttgcctaccc aaaacatctg ctgcagctgc
      781 tgcgaaaggg actctactgc gccgagaacg acatcgtcca acacgggggc gggattcccg
      841 ttcttcatac gattgttcac acgaacaaca ctcctttctg aggccggtac gaaacgcgcg
      901 ctgcttcttt tgacgggttc cctcttttcg tcatgtggac attatgaatc tagaaaacgc
      961 tccacagtgc gaggagacca aaaacaccga tgattacccc ggaaagtcta ttgagccatc
     1021 tgagaccgct ggcggtgaat ttctcccgga acaggccgaa gccgccgctc aaaagaagcc
     1081 accagagagc tgaacccgca aacaccccga ggacgagtac tccggcggat atatagtttc
     1141 cgctcccttc ggccagtccg agtccggcaa atatggcggc gaacgaaata atcgtcatcg
     1201 gattagtgag cgtcaggaac aaggtggaga gatacgcccc aagtaggccg tgtccgcgta
     1261 cctcggccgc tttttgtgcg ggctgtgcga aaaacgtctt gaccgccaga tacagcagga
     1321 aaagacctcc ggcaaggcga agctccgtct gcctgctgac cagaaagttc gagagaaacg
     1381 tcaagccgaa gcccgcgata cacccgtaca ccgcatccgc actcgccgcg cccagtcctg
     1441 aaacaagacc tgacgcacgc ccttcggtca gcgtgcgccg gatacataaa actcctatgg
     1501 gaccgacagg cgcggcgatc gaaaacccga taacaacgcc tctgaacaat atcgagagat
     1561 ccatcttgct cctccgtttc cagaatgggg gccttccggg aagtcacccc gtcaatatac
     1621 cactttccta tcaaaagaca agggaataaa gttgattcta tggacaattc aacgagtatt
     1681 catgatatcg attttttaaa aaagccagag gcaggcgagt ccggcggcaa ggctgatcag
     1741 ggtcaccgga actcccacga gcgcgtgctg cttccagtcg aatggaatcc ccatacgctc
     1801 cgcttcgcct gcgacgatga tgttggcgat gctccccacg atcagcagat ttcccgcgaa
     1861 ggtgctcgac accgcaagga tgtaaccggc ctgcggaacg tcgcgcacaa gtgggagcag
     1921 cagcatgacg gccggaacat tggagacaag attcgagagc actgtcgtca ctccgtaaag
     1981 ccaggccgga gaggaaagcg atacccctgc tgattcaagg aactctttga gaatctgaag
     2041 acctccggca gactcgaacg ccgcattgac gacgaaaagg ccgaggaaaa ggagaagaag
     2101 ctgccagtcc actatttcga gggtccgccg cgacgccatc ttcctgctga gcagaaggac
     2161 ccccgcagcg ccgagcgccg tgagatcacg aggaagcgtc gagaagagaa acaagcccac
     2221 gactaccgtc aggacgacac cccccttcaa cgtctgccag gcatcaaagg agcgctcatc
     2281 aaccagctgc tcatccattt ggagcgtcca gcgtccccgg aaaaggaaga gcatgatccc
     2341 ccagagtgaa agcagcgaca ggaacacggg gaaggcggcg aagagcagat atccgccgaa
     2401 aggaagcgag agatactggc cgatgagcat gttctgcgga tttccgatga gcgtgagcgc
     2461 ggaaccgata ttcgcggcgc acgcaagcgc aaggaaaaaa ggaagagggt tgagcccccg
     2521 gcggatgcac cccgccccca gaacgggcgt catggcgaga cagaccacat cgttcgtgag
     2581 gagcgccgac atgagagcgg aagctccgat gaccgccccc atgataagcg aaggaggcgc
     2641 cgcgagagcc cctatgcgcc gggaaacctc ggagtagaaa cctcccagac ggaactgcgc
     2701 cgaaataacc ataaagccga acaagagcaa aattgtgggg acgtcgatcc cggagcggcc
     2761 gccctcgaga gacgcctttc ccgcggcaag aagcacgatt gcgcccaaaa ggacaatacc
     2821 ggtgcggtca agcgcgaggc gaggaaagcg cccaaagatc atccccagat agacggcggc
     2881 aaaaacaaaa aagacgaaga acccattcac cacaggaaac acctcgcaga aataagtaaa
     2941 caacaagagg gaattatact cccgaacagc gtgctctgga aagcttggaa tgagcgtgcc
     3001 gttccggatg tacccacaat tttcatccca aagcctactt ttgcaccccg ggaacgtaaa
     3061 tttacgtaaa aagtctggaa atagatgctt gacaggatgc agcaggggct gtatcatccc
     3121 tatcaatatt cagacagaca cttcaccgga aaggaggcca ttttgatgaa tgcacagaga
     3181 ttgagtttgg ttcgtatttc tgttcttgta ctcgtcctcg tactcctcat tatcggcggt
     3241 cccccttccg gtgggttcgg cgcataagcc gtacacctaa aaccggaacc gggggccgcc
     3301 tttctggagg cccccggttt ttttattggg aagtgttttt actctgcgtc tttttataat
     3361 tttgaaggga gtgtgtacaa tggcaggaaa gtacggattc acagcggcga cggaaacaga
     3421 gagagaggtc ttcagaagaa atgatcgtca tctcgacgat atgttctcgg tcagttggag
     3481 agggcccttt gaaggattct ccgcttcaag gaaggccgat cttgcggaac cctccttctc
     3541 acagacggag atgcggtggc gcggcccgtt cgaaggactt cgctaggctt aaaaagaagc
     3601 gggaaaatga cggaagcgtc ccccgatcct gtcatccctt cccgcttcaa taccccgact
     3661 cggggtggcg aaaacagctc gtcaggtgat cgttcaccat tcccgtagcc tgcatatgcg
     3721 cgtaacaaat caccggaccg aagaagacga acccctcttt tttcatgcac ccgcttactt
     3781 tctccgcgag cggggtcaca gcaggcactt cttccatggt tttccatctg ttgacgatcg
     3841 gctccccgtc gaggaaatcc cataagtatg ccgaaaaaga gccttt
//
LOCUS       DAOTIL010000161         3882 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminobacteriaceae bacterium isolate AMR_MDS_3026
            NODE_27734_length_3882_cov_4.041808, whole genome shotgun sequence.
ACCESSION   DAOTIL010000161 DAOTIL010000000
VERSION     DAOTIL010000161.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868050
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminobacteriaceae bacterium (wastewater metagenome)
  ORGANISM  Aminobacteriaceae bacterium
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae.
REFERENCE   1  (bases 1 to 3882)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3882)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:19:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,224
            CDSs (total)                      :: 3,198
            Genes (coding)                    :: 3,184
            CDSs (with protein)               :: 3,184
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3882
                     /organism="Aminobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_27734_length_3882_cov_4.041808"
                     /isolate="AMR_MDS_3026"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:3034609"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..885)
                     /locus_tag="P5201_04550"
     CDS             complement(<1..885)
                     /locus_tag="P5201_04550"
                     /inference="COORDINATES: protein motif:HMM:NF019604.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HRV97835.1"
                     /translation="MAKERILIVGGDAAGMSAAGQIRKLLPDSEIVVYERSGYSSYSA
                     CGIPYFIAGLVEPETRLVVRTPREFLEKQNIVVHVRHEVLSLDPGAGKVKVRDLETGR
                     EFADGFDKLLLATGASPVVPPLNGAKADGIFSLGVLETGIAARKFIDQHRPRHAVVIG
                     GGYIGLEMAEAFACVRGIKVTLIDKAPQVMSTFDPDMADHIAEAIRGIGTELRLGETL
                     LGFGTEGGRVRSVITEGGTIPTDMVIMGLGVRPNSQLAEEAGQRRAEVVGDAFEVDDD
                     SSHAVLADERNRTCNRVRA"
     gene            complement(961..2220)
                     /locus_tag="P5201_04555"
     CDS             complement(961..2220)
                     /locus_tag="P5201_04555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005660919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HRV97836.1"
                     /translation="MPDWEKSTLFRAFRHRNFRLFYMGQIVSLSGLWMQRVAMNWLVY
                     RITGSPLLLGTVDFMAQIPVLLFGSFTGAFLEGRNLRKVIMICQTISMLHALTLAILT
                     LSGVVEYWHILILAAVIGTSDSFEVPARQGFTIRLVEDEQDLGNGIALTSTLFNVTRL
                     IGPSVAGMVIALVGEGICFLMNGMAYSATLTALFLMRFRRPLVSESTKKGVLEGMKEG
                     IRYVAGFLPLRNYLSAIALVSFFAFPYMVLLPVFAREILGGGPRTLGFLMGATGLGAL
                     AGSVRLALRKSPVGLGKIMAVSCILLGITMALFSQSGIFPLSLLLMACLGFFMVSTLV
                     SCNTLVQTLVDEDKRNRVMSLFIVCAMGITPLGSLNAGWLASNIGAPTTLLAGGAVSF
                     LIGLWLLRSWPVMWALSEPVYRKKNLL"
     gene            complement(2227..3111)
                     /locus_tag="P5201_04560"
     CDS             complement(2227..3111)
                     /locus_tag="P5201_04560"
                     /inference="COORDINATES: protein motif:HMM:NF021445.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylglycerol lysyltransferase
                     domain-containing protein"
                     /protein_id="HRV97837.1"
                     /translation="MSLTFSPLSIDMRSDYTPLFDKCSEKTSDYTFVNIWAWSDERKY
                     ELAFARDLFWPRVTSSAPVYWAPVGNWEATDWESVLPELFPDGMTFDRVPEKLAQLWS
                     ERLGDRVTLEEQRSEWEYVYSVEELVALRGNRFHKKKNLLKQFQREYVSEYKALGRDD
                     VEEILEMQQLWCEWKSCDDIPGLRAENHAISRVLKKWDLLPGLLCGALYVKEYMVAYA
                     VGEPMTDDMVVVHFEKGLAEYKGVYQAINQSFLANSCSGFAWANREQDMGEEGVRKAK
                     ESYNPSHFLKKYRAVWKG"
     gene            complement(3245..>3882)
                     /locus_tag="P5201_04565"
     CDS             complement(3245..>3882)
                     /locus_tag="P5201_04565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013049176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="Hsp70 family protein"
                     /protein_id="HRV97838.1"
                     /translation="IFSTAADNQPAVDIVVLQGEREMAKDNRILGNFKLEGIRQAPRG
                     LPQIEVAFDVDANGILNVTAKDKDTAKEQRITINGSTNLDKSEVERMIRDAQENAAED
                     RKKKELAEARNELETVVYQLEKALEQAAANVPENEKARARNLVEEGKSALSKSDATLD
                     ALRVASSDIKQMLQILASSNAGGPEPQPASSARSGDDVVDAEFVDNSENRG"
BASE COUNT          813 a         1210 c         1078 g          781 t
ORIGIN      
        1 cgcgcgcacg cggttgcaag tgcggttgcg ctcgtccgcg agcacggcat gggaggaatc
       61 gtcgtcgact tcgaaagcgt ccccgacgac ctcggcccga cgctgtccgg cttcctccgc
      121 gagctgcgag ttgggacgta ctccaagtcc cataatcacc atgtcggtgg ggatggttcc
      181 cccctcggtg ataacggaac ggactctccc tccctcggtg ccgaaaccga gcagcgtttc
      241 gccaagacga agttccgtcc cgatgccgcg tattgcctcc gcgatatgat ccgccatatc
      301 ggggtcgaag gtgctcatca cctgcggcgc cttgtcgatc agggtgacct tgattccacg
      361 gacgcaggcg aaagcctccg ccatttcaag gccgatatac cctcctccga tgaccactgc
      421 gtggcgcggg cgatgctggt cgataaactt gcgtgcggcg atgcccgtct caaggacgcc
      481 gaggctgaag ataccgtccg ccttggcgcc gttcagcggc ggcacgacgg gactcgcgcc
      541 ggtcgccagg agcagtttgt cgaaaccgtc ggcaaactct ctgcccgtct caagatcgcg
      601 aaccttcacc ttgcctgcgc cgggatcaag cgaaagcact tcgtgcctga catgaacgac
      661 gatattttgc ttttccagga actcccgggg cgtgcggacc accaggcgcg tttccggttc
      721 gacgagcccg gcgatgaagt aagggattcc gcacgctgaa tatgaggagt agccgcttcg
      781 ctcatagacg acaatttcag aatcgggcag cagtttcctg atctgacccg cggcggacat
      841 gccggcggcg tcgccgccga cgataagaat acgctctttc gccattccga tcacctccgc
      901 aataccttaa atgggtattt ttttcttctt cagagtatac cccaaatccg ggggaacgcg
      961 ctacagcaga ttcttctttc tatacaccgg ctccgagagc gcccacataa cgggccacga
     1021 acgtaacagc cacaagccga tgagaaaact gacggcgcca cccgcaagaa gggtcgtcgg
     1081 cgctcctatg ttcgaggcga gccatccggc gttgaggctc cccagcggtg ttatgcccat
     1141 cgcgcacacg atgaagaggc tcatgacgcg gtttcgtttg tcctcgtcca caagagtctg
     1201 gacgagcgta ttgcaggaaa cgagcgtgga gaccatgaaa aaaccaaggc acgccatcag
     1261 gagcagcgaa agagggaaga tcccggattg ggaaaagagc gccatcgtaa ttcctaaaag
     1321 aatgcaggag acagccatga tcttgcccag tccgactggc gactttcgca gggcgagacg
     1381 gaccgacccg gcgagcgcgc caagcccggt tgcgcccatc aggaaaccaa gcgtccgggg
     1441 tccgccgccg agaatctcgc gggcaaagac ggggagcagc accatgtacg ggaaggcgaa
     1501 gaagctcaca agagcgatcg cggagaggta gttcctcagg ggcagaaagc ccgcgacgta
     1561 acgaattccc tccttcatcc cttcgagcac ccccttcttc gtactttccg aaacaagcgg
     1621 acgtctgaag cgcatcagga agagcgccgt gagcgtcgcc gaataggcca taccgttcat
     1681 caggaagcag atgccctccc ctaccaatgc gatgaccata ccggcgaccg acggaccgat
     1741 gagacgagtg acgttgaaaa gagtggacgt gagggcgatg ccgttgccga gatcctgttc
     1801 gtcctccacc agacgtatcg taaacccttg ccgggccggg acctcgaacg aatccgacgt
     1861 tccgatgacg gcggcgagta tcaggatatg ccagtactcg acaacgccgc ttaacgtcag
     1921 aatggcaagc gtaagggcgt ggagcatgct gatggtctga cagatcatga tgaccttccg
     1981 caggttccgc ccctccagaa aggcccccgt aaaagagccg aagagcagca cgggaatctg
     2041 cgccatgaaa tcgaccgtcc ccaaaagcag cggcgaaccc gtgattctgt agacaagcca
     2101 gttcatggca acccgttgca tccagagtcc ggagagcgag acgatctgcc ccatgtagaa
     2161 caggcggaaa ttccggtgcc ggaaggcccg gaagagcgtg ctcttctccc agtcgggcat
     2221 atcgctctac cccttccaga cggcgcggta cttcttaagg aagtgcgacg ggttgtacga
     2281 ttccttggcc ttccgaaccc cctcctcgcc catgtcctgc tcgcggttgg cccacgcgaa
     2341 gccggaacag gagttcgcca ggaaggactg gttgatcgcc tggtagacgc ccttgtactc
     2401 cgcaagcccc ttctcgaaat gcaccacgac catgtcgtcc gtcatcggct cgcccacggc
     2461 gtaggctacc atgtactctt taacgtagag cgcgccgcag agcaaccctg gtaggagatc
     2521 ccacttcttc aagacacgcg aaattgcgtg gttctccgcc cgaaggccgg ggatgtcgtc
     2581 gcagctcttc cactcgcacc aaagctgctg catctcgagg atttcctcaa catcgtcgcg
     2641 tccgagcgcc ttatattcag aaacgtactc cctctgaaac tgcttcagga ggttcttttt
     2701 cttgtgaaaa cggtttccgc gaagcgccac aagctcctcc acggagtaga cgtactccca
     2761 ttccgaacgc tgttcctcca gcgtcacgcg gtcgcccaga cgttcgctcc aaagctgcgc
     2821 gagtttctcc ggtacccggt cgaacgtcat gccgtcaggg aagagctccg gcagcacact
     2881 ctcccaatcg gtcgcctccc agttacccac cggcgcccag tacaccggag cggaactcgt
     2941 cacacgaggc cagaaaagat ccctggcgaa agccaactca tacttgcgtt cgtccgacca
     3001 tgcccagata ttcacgaacg tgtagtcgga tgttttttcg gaacatttgt cgaaaagagg
     3061 ggtgtagtcg ctcctcatat ctatcgaaag cggcgaaaaa gtcaatgaca tcctgtcacc
     3121 tccagtaatg aaagtagtat acccgtaaag aagcctgcgg gcaatttccc gcaggcttct
     3181 ctctattgaa aaccggcgtt tcgccggata tgtttttcag gccgccggcc ctccgctaaa
     3241 agcctcagcc gcggttttcg gagttatcca caaattcggc gtccacaacg tcgtcgccgc
     3301 ttctagccga ggatgcgggc tgcggttcag ggcctccggc gttcgacgac gccagaattt
     3361 gcagcatctg cttgatatcg gaggaggcga cgcgaagggc gtccagcgtt gcgtcgctct
     3421 tggacaatgc agattttccc tcctccacga ggttacgggc ccgggccttc tcgttctccg
     3481 gtacgttcgc cgccgcctgt tcaagcgcct tctccaactg gtagacaacc gtttccagtt
     3541 cattccgggc ctcggcgagc tctttcttct tcctgtcctc ggcggcgttc tcctgggcat
     3601 cgcggatcat gcgctccact tcggatttgt cgagatttgt cgacccgttg atggtgatac
     3661 gctgctcctt tgcggtatcc ttgtccttcg ccgtgacgtt caggatgccg ttggcgtcga
     3721 catcgaaagc cacttcgatc tgcggcaacc ctctgggggc ctgccgtatt ccctcaagct
     3781 tgaagttgcc gaggatgcgg ttatccttcg ccatctcacg ttcgccctgg agcaccacga
     3841 tgtctaccgc aggctggttg tccgccgctg tcgagaatat tt
//
LOCUS       DAOTIL010000162         3876 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminobacteriaceae bacterium isolate AMR_MDS_3026
            NODE_27788_length_3876_cov_3.654279, whole genome shotgun sequence.
ACCESSION   DAOTIL010000162 DAOTIL010000000
VERSION     DAOTIL010000162.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868050
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminobacteriaceae bacterium (wastewater metagenome)
  ORGANISM  Aminobacteriaceae bacterium
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae.
REFERENCE   1  (bases 1 to 3876)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3876)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:19:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,224
            CDSs (total)                      :: 3,198
            Genes (coding)                    :: 3,184
            CDSs (with protein)               :: 3,184
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3876
                     /organism="Aminobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_27788_length_3876_cov_3.654279"
                     /isolate="AMR_MDS_3026"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:3034609"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..259
                     /locus_tag="P5201_04570"
     CDS             <1..259
                     /locus_tag="P5201_04570"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRV97839.1"
                     /translation="VAAEEPKAEEPAVEEVKSEPVSGAPVAVEEPKPEEPAAEAEQET
                     AVEEAAKEAAEVPAPESPAPETVQQDKQTEKPAGATAAPCD"
     gene            469..2328
                     /locus_tag="P5201_04575"
     CDS             469..2328
                     /locus_tag="P5201_04575"
                     /inference="COORDINATES: protein motif:HMM:NF013481.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde ferredoxin oxidoreductase family
                     protein"
                     /protein_id="HRV97840.1"
                     /translation="MYGYWGKLLRVDLTHRTHRIEDIPEEVFDLLLGGSALGAKILLE
                     ETPANVDPFSPENKLIFSVGPFQGVNFPGNGKWSVITKGPLTGTYLESAGTGHWAPYF
                     KKCGYDAVVFEGKASSPVYVVLRDDSVEFRDASFIWGKDTVETGELIKEDLGDKRINA
                     LNIGPAGEIGNPIACITCDGHSFAGRGGAGAVMGSKNLKAVAAWGTKEVPVFDKEKAS
                     EYSKEIFKLLHEQGEGFRQHGTPVVMVPLEEIGDVPIKYWRGDENKYGAYLLGAPRYT
                     EYLNVKPLPCINCPVGCHRHIHFEFPDGTVLDGNGPEYETIGMMGTNLLMEDLSAVAV
                     ANDIANRMGIDTVSCGAWIGFLMECWEYGWITPKETDGVTIKWGDPEVLVELTQQIAS
                     MSGVGEWFREGVRGAAKKIGKDAEKITVEVKNLDYPAHDPRAVYGLGVNYAVGTRGAC
                     HERGNPQASALGLFFPELGQDAPYDRQDPKFAAKTAILHQDVSALHNCMTLCKFMMGG
                     AGLTLTEMNTAFKHLTGKDVPVPEFVKYGERAWQLQRLINVRDGMNRKDDTLPLKMTI
                     PAMVGPRAGKAPTPHDEILDDYYAQRGWDKNGVPTKERLEALGLGAYVKYLGR"
     gene            2354..3511
                     /locus_tag="P5201_04580"
     CDS             2354..3511
                     /locus_tag="P5201_04580"
                     /inference="COORDINATES: protein motif:HMM:NF020084.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FIST N-terminal domain-containing protein"
                     /protein_id="HRV97841.1"
                     /translation="MMQAKVGWSTAGSPLAAGKEAAQAAKVDGAQIALLFGSVDYNAE
                     ELLKGVRETLGDVPVIGCTSYTGVLTPGGFITSPDGYCALMALSDEDMAVSVGAAEKK
                     ECAVAAGEEAALAALEAAGMDMAPEFFYMVAPPGEEEFYLQGIQSVIGRVPFFGGSAA
                     DNDLSGKWLVYGNGVVKNGVAVAFFWNKAYGNKYTGAYRGTGKRGIITKVENNRVLKE
                     IDGKPALDVLAGWLGNTAADLQGFNLLGATIHHPIGITDFAGGHVWIRHPMVGNADGS
                     ANIGNNLAEGVAIELMEATTDELVASVESTAKAAVEMLGDEGLGALVAVHCGGRRGGI
                     GDRMDETVTAMKNAIGETPFIGVFTFGEYGFEGDLANGCGGLMLSFMALGK"
     gene            3514..3738
                     /locus_tag="P5201_04585"
     CDS             3514..3738
                     /locus_tag="P5201_04585"
                     /inference="COORDINATES: protein motif:HMM:NF014636.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MoaD/ThiS family protein"
                     /protein_id="HRV97842.1"
                     /translation="MPARLHLLGIFKKYQPSDPWEVQPGDQSVELLLKEAGMDSHAGY
                     VLLVNGSRKKKDYVPQEGDEIKVMPLVAGG"
BASE COUNT          894 a         1019 c         1228 g          735 t
ORIGIN      
        1 cgtagcagca gaggaaccga aggctgaaga gcccgcagtc gaagaagtga agtctgaacc
       61 tgtttccggg gcgcctgttg ctgtggagga accgaagcct gaagaacccg ctgccgaagc
      121 ggagcaggaa acagcagtgg aagaggcggc aaaagaagcg gcagaggttc cggcgcctga
      181 gagccctgcc ccggagaccg tacagcagga caagcaaacg gaaaagccgg caggggcaac
      241 agcagctccc tgcgactgag ctccgggaca ctcaccaaaa gaacctgttt tgattgcaac
      301 aagaagagat tcattgaaaa gcctccgtgt ttcctgcgga ggcttcttcg tacaaaaaaa
      361 atataatagt tgcaaagttg gagctttgcc tgtagactga tattgtttgt tgaactgttc
      421 atctaataga gttccaacat actgtaccga ttttcaggag gtggagcaat gtacgggtat
      481 tggggtaaac tgcttcgggt cgacctgaca catcggacgc acagaatcga ggatattccc
      541 gaggaggtct tcgatctgct gctgggcggc tctgcgctag gagccaaaat tctgctggag
      601 gaaactccgg cgaacgtgga tcccttttca ccggagaaca agcttatttt ctccgtcggt
      661 ccgttccagg gggtgaattt cccggggaac ggcaagtgga gcgtgatcac caaaggtcct
      721 cttaccggaa cgtacctgga gtcggccggt accggacact gggcgcccta cttcaagaag
      781 tgcggctacg atgcagtagt cttcgaagga aaggcgagca gccctgtcta tgtagtgctc
      841 cgcgacgatt ccgtcgagtt ccgcgacgcg tcctttatct gggggaagga caccgtcgag
      901 accggcgaac tgatcaagga ggatctgggc gacaaacgta tcaacgcgct caatatcgga
      961 cctgccgggg agatcggcaa tcccatagcc tgcattacct gtgacgggca cagtttcgca
     1021 ggcaggggag gcgcaggcgc ggtcatgggt tccaagaact tgaaggccgt tgccgcatgg
     1081 gggacgaagg aagtccccgt tttcgacaag gagaaggctt ccgagtactc caaggaaatc
     1141 ttcaagctcc ttcacgaaca gggggagggg ttccgtcagc acggaacgcc ggttgtcatg
     1201 gtcccgcttg aagaaatcgg cgacgtgcct atcaagtact ggcgcggaga cgagaataaa
     1261 tacggggcct acttgctggg agctccccga tacaccgagt atctcaacgt taaacctctg
     1321 ccgtgtatta actgtccagt cggctgtcat cgtcacatcc atttcgagtt tccggacgga
     1381 acggtactcg acggcaacgg tcccgagtac gaaaccatcg gcatgatggg aacgaacctg
     1441 ctcatggaag acctgagcgc cgtcgccgtc gccaacgata ttgcaaaccg catggggatc
     1501 gacaccgtct cctgcggcgc atggatcggt tttctcatgg agtgctggga gtacggctgg
     1561 atcacgccga aagagaccga cggcgtcacg atcaagtggg gagatcctga agttctcgtc
     1621 gaattgacgc agcagatcgc ctccatgagc ggcgtaggag agtggttccg cgagggcgtc
     1681 cgcggcgcgg cgaagaagat cggcaaggat gcggagaaga tcacggttga ggtgaagaat
     1741 ctcgactatc cggcgcatga cccccgcgca gtctacggat tgggcgtgaa ctatgccgtc
     1801 ggaaccaggg gagcatgtca cgaacgaggc aatccccagg cgagcgcgct tggactcttc
     1861 ttccctgaat tggggcagga cgctccgtac gaccgccagg atccgaagtt cgcagcaaag
     1921 accgcgatac ttcatcaaga cgtgagcgcg ctgcacaact gcatgacgct ctgtaagttc
     1981 atgatgggcg gcgcagggct tactcttacc gagatgaata cggccttcaa acatcttacc
     2041 ggaaaggatg ttccggttcc cgagttcgtg aagtacggcg agcgcgcctg gcagcttcaa
     2101 cgcctcataa acgtccgcga cggcatgaac cggaaggacg acacgctccc gttgaagatg
     2161 accatcccgg cgatggtagg gccaagggct ggaaaagcgc cgactcctca cgacgaaata
     2221 ctggacgatt actatgcgca gcgcggatgg gacaagaacg gcgtcccgac gaaggaacgc
     2281 ctggaagcgc tcgggcttgg agcatacgtg aagtatttgg gccggtaggc ggagcgggga
     2341 ggtcgcgaag attatgatgc aggcaaaagt tgggtggagt acagccggtt cgcccttggc
     2401 tgcgggaaaa gaggcggcgc aggcggcgaa ggtcgacggc gcccagatcg cgttgctctt
     2461 cggaagcgtc gattacaacg ccgaggagct cctgaaagga gtgcgtgaaa cgctgggcga
     2521 cgttcccgtc atagggtgta catcctatac cggcgttctt accccgggag gttttatcac
     2581 atctcctgac ggctactgcg ctctgatggc tctctcggat gaggatatgg cggtttccgt
     2641 cggtgcggcg gaaaagaaag aatgcgcggt tgccgcggga gaagaggcgg ctttggccgc
     2701 gctggaagcc gccggtatgg atatggcgcc cgagttcttc tacatggttg cccctccggg
     2761 agaggaggag ttctaccttc agggaattca gtccgtcatc ggcagagttc ctttcttcgg
     2821 cgggagcgcg gccgacaacg acctgtccgg aaagtggctc gtctacggca acggcgtggt
     2881 caagaacggg gtcgcggtgg ctttcttctg gaacaaggcg tacggcaaca agtacacggg
     2941 cgcctacaga ggcacaggaa agcgcggcat catcacgaag gtggagaaca accgcgttct
     3001 caaggagatc gacggcaaac ctgcgctcga cgtgctcgcc ggatggcttg gaaacaccgc
     3061 cgccgatctg caggggttca acctcttggg agccaccata catcacccca taggaatcac
     3121 cgatttcgcc gggggacacg tctggatccg ccatccgatg gtcggcaacg cggacggcag
     3181 cgcgaatatc ggaaacaacc tcgccgaggg cgtggcgatt gagcttatgg aggcgactac
     3241 cgacgagctt gtggcgtctg ttgagagcac cgcgaaagcc gccgtggaaa tgctcggaga
     3301 cgaggggctc ggagcgctcg tggccgttca ctgcggcgga cgtcgcggag gtatcggcga
     3361 ccggatggac gaaaccgtaa cggcgatgaa aaacgccata ggcgagactc cattcatcgg
     3421 cgtcttcaca ttcggcgaat acggctttga aggagatttg gccaacggct gcggcggtct
     3481 catgctctcc ttcatggcgc tggggaagta gccatgccgg cccggctgca tctcctcggc
     3541 atctttaaaa agtaccagcc ctccgatccc tgggaggtcc agccgggcga tcaatcggtg
     3601 gagctgctgc tcaaagaggc gggaatggac tcgcacgcgg ggtatgttct tcttgtgaac
     3661 ggttcacgga agaagaagga ctacgttcct caggagggcg atgaaataaa agttatgccg
     3721 ctcgtcgccg ggggatgata gacaagtctg tcggcgtcat gatagaattg cgacatggta
     3781 catgatgcgg agtatccgaa ggactccatc cttgtggtgg gaaattccca aacgaccggt
     3841 ttcaacccta ttaaccagca gttcggtcaa tttttt
//
LOCUS       DAOTIL010000163         3875 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminobacteriaceae bacterium isolate AMR_MDS_3026
            NODE_27802_length_3875_cov_4.150262, whole genome shotgun sequence.
ACCESSION   DAOTIL010000163 DAOTIL010000000
VERSION     DAOTIL010000163.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868050
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminobacteriaceae bacterium (wastewater metagenome)
  ORGANISM  Aminobacteriaceae bacterium
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae.
REFERENCE   1  (bases 1 to 3875)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3875)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:19:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,224
            CDSs (total)                      :: 3,198
            Genes (coding)                    :: 3,184
            CDSs (with protein)               :: 3,184
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3875
                     /organism="Aminobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_27802_length_3875_cov_4.150262"
                     /isolate="AMR_MDS_3026"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:3034609"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..428)
                     /locus_tag="P5201_04590"
     CDS             complement(<1..428)
                     /locus_tag="P5201_04590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014162530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="imidazolonepropionase"
                     /protein_id="HRV97843.1"
                     /translation="MIVKFFRNARIYTPEGKPPSRGREQGALRVHERGAMLVQDGVLE
                     AVGTEEELLSNPLVKKVDEEIDCEGRCLIPGFVDPHTHMCFAARREEEFSMRLAGAAY
                     LDILKKGGGILSSVRHVRGASEQEIFERTRTLALSALSFGT"
     gene            complement(425..1339)
                     /gene="ftcD"
                     /locus_tag="P5201_04595"
     CDS             complement(425..1339)
                     /gene="ftcD"
                     /locus_tag="P5201_04595"
                     /EC_number="2.1.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008521125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030409 - glutamate formimidoyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0030412 - formimidoyltetrahydrofolate
                     cyclodeaminase activity [Evidence IEA];
                     GO_process: GO:0019556 - histidine catabolic process to
                     glutamate and formamide [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate formimidoyltransferase"
                     /protein_id="HRV97844.1"
                     /translation="MAGKLIECVPNFSEGRRQDVIEAIVAPFRATKGCGLLDYRADAD
                     HNRLVVSLAGSPEPVQEALLKAAAVAVQKIDMNSHQGAHPRIGAVDVIPFTPVSNISM
                     EECVALSHSFGERYWKELGVPVYFYEDSAKRPERKRLEVIRKGQYETLKTESVTPERA
                     PDVGGPALHPTAGATVMGARKFLVAFNVNLNTADEEIAKKIANCVRASSGGFCHVKGM
                     GVALHERGMAQVSMNIVDFEKNAVYRVLELIRMEARRWGVEVVETEIYGMIPAQALVE
                     SAAYYLQIKDFDPMQVLELKLLSGGDAE"
     gene            complement(1472..2656)
                     /locus_tag="P5201_04600"
     CDS             complement(1472..2656)
                     /locus_tag="P5201_04600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005659449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HRV97845.1"
                     /translation="MNPQFARRMTRFQESDLTPILRLAANPEVISFAGGLPAPELFPV
                     EDLKKVAVKVLDANGRRALQYSATEGHPPLREKIAARTNRNLKTSLTAGNVVITTGSQ
                     QSLDILAKMYIDEGDCILCESPTYLGALSAFNSCLPAYREVATDGEGMIIADLEKALA
                     SEKRAKMIYVIPDFQNPSGKTWSLERRKAFLDTVSKYDILVVEDNPYGELRFEGTTPP
                     SLKSMDTRGQVVMLGTFSKIFCPGVRVAWVCGEPAFLESFCNLKQAADLHTSTISQYE
                     IDAYLSDYDIEEHIKKLVSVYRERRDLMIRTMDASFPKEVVFTRPEGGLFTWAEMPEH
                     VNARDLFLKALEKKVAFVPGGAFFPNGGHENTMRLNYSNMPPERIVEGIERLGAAIRE
                     YL"
     gene            complement(2786..>3875)
                     /locus_tag="P5201_04605"
     CDS             complement(2786..>3875)
                     /locus_tag="P5201_04605"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HRV97846.1"
                     /translation="EEEDEHRISVAFAVSDTGPGIPQSKVHLLFDDFTQLDGSSTRKH
                     GGTGLGLAISRSLARLMGGDITVESEVGRGSVFRFSLPLLRCSGEIVEEHEGTETDFS
                     SMKGNVLLAEDNAVNTKMTVAMLKKTGLTVRHAQNGREAVEMWRSEPFDLILMDCEMP
                     VMDGLEATRRIRSEEQDGKSIPIVALTAYAMKGDRERCLEAGMTEYISKPIRSRKLYS
                     LLARFLGTPSPSLHTDSEETPARFSAENGERERWESGFAELLLSLDGDREALAEMVEA
                     FFSEVPELRGSLRSSLEKGDSSAAARALHKLKGVLGYLMGGEDISLFRVLEIDARTEG
                     LKTDDPRLRELHDLLDRYELFLREAASR"
BASE COUNT          759 a         1215 c         1105 g          796 t
ORIGIN      
        1 gtcccgaagg agagcgcgga gagcgcgagc gtcctcgtgc gctcgaagat ctcctgttcg
       61 ctcgcgcccc gtacatgccg gacggaggag agaatgccgc cgcccttctt gagtatgtcg
      121 aggtacgccg cccccgccag gcgcattgag aactcctctt cccgccgcgc ggcgaagcac
      181 atgtgcgtgt gcggatcgac gaagccgggg atcaggcagc gcccttcgca gtcgatctct
      241 tcgtcgacct ttttcacgag gggattgcta agaagctcct cttccgtgcc gacggcttcg
      301 agaactccat cttgaacgag catcgcccct cgttcatgaa cgcgcagagc cccttgctca
      361 cgccccctgg agggaggctt tccctccgga gtgtagatgc gggcgttgcg gaagaatttg
      421 acgatcattc agcgtcaccg ccggagagga gcttcagctc cagaacctgc atcgggtcga
      481 agtccttgat ctgcaaatag tacgctgcgc tctccacaag agcctgcgca ggaatcattc
      541 cgtagatctc cgtctcgacg acctcgacgc cccatcgtct ggcctccatc cggatcagtt
      601 cgagcaccct gtacaccgcg ttcttctcga agtcgacgat attcatgctc acctgcgcca
      661 tgccgcgctc gtggagcgcc acgcccatgc ccttgacatg gcagaagccg ccgctggagg
      721 cgcggacgca gttggcgatc ttcttcgcga tctcctcgtc cgcggtgttc aggttgacgt
      781 tgaaggccac gaggaacttc cgggcgccca tcaccgtcgc gcccgccgtc gggtgcaggg
      841 cgggaccgcc gacgtcggga gcgcgctccg gtgtgacgga ctcggtcttg agcgtctcgt
      901 actgcccctt gcggataact tcaaggcgct tccgttccgg gcgtttcgcg gaatcctcgt
      961 agaagtagac cggaacgccg agttctttcc aatatctctc gccgaacgaa tgcgagagcg
     1021 ccacgcactc ctccatagaa atgttgctca cgggggtgaa aggaatcacg tccaccgcgc
     1081 cgatgcgcgg atgcgccccc tggtggctgt tcatgtcaat cttctgcacc gcgacggcgg
     1141 cggccttgag aagcgcctcc tgaacaggct cgggcgatcc cgcgaggctc acgaccagcc
     1201 tgttatggtc cgcgtccgcc cgataatcga gaagaccgca ccccttcgtc gccctgaacg
     1261 gagcgacgat cgcctcgatc acatcctgac gccgtccttc gctgaaattc ggaacacact
     1321 cgatgagctt gcctgccatg ggaacaacct cctgcgggta ttagtatgct gcacggtaca
     1381 tgataacatt gaaacatgta caacgtaaag cgcgaaagta aaaagagagg caggacccct
     1441 tgtttcagcc ctgcctctct cattcgtttt tttagagata ctcgcggatc gccgctccca
     1501 ggcgttcgat cccttcgacg atgcgctccg gcggcatgtt ggagtagttg agccgcattg
     1561 tgttctcgtg ccccccgttg gggaagaacg ctcctccggg gacgaaggca accttcttct
     1621 cgagcgcctt gaggaagagg tcccgcgcgt tcacatgctc cggcatctcg gcccatgtga
     1681 agagccctcc ctcgggacgc gtgaacacaa cctccttcgg gaatgatgcg tccatcgtgc
     1741 ggatcatcag gtcgcgccgc tcgcggtata cggagaccag cttcttgatg tgttcctcta
     1801 tatcgtagtc ggagaggtag gcgtcgatct cgtactggct gatcgtcgac gtgtggaggt
     1861 cggcggcctg tttcagattg cagaagctct caaggaacgc gggctccccg cagacccacg
     1921 ccacgcggac gccggggcag aagatcttcg agaaggtgcc cagcatcacg acctggccgc
     1981 gggtatccat cgacttcaag gaggggggcg tcgtcccttc gaagcggagc tcgccgtacg
     2041 ggttgtcctc caccaccagg atatcgtact tcgagacggt atccagaaac gccttgcgcc
     2101 gttcaagcga ccacgtcttg ccggaggggt tctggaagtc cggaatgacg tagatcatct
     2161 tcgcgcgctt ctccgaggcg agcgccttct caagatcggc gatgatcatt ccctcgccgt
     2221 ccgtcgccac ttcccggtac gccggaaggc acgagttgaa ggccgaaaga gcgccgaggt
     2281 aggtgggact ctcgcacaga atgcagtccc cctcgtcgat gtacatcttc gccagaatat
     2341 ccagcgactg ctgggacccg gttgtgatca cgacgttccc ggccgtcagc gacgtcttca
     2401 ggtttctgtt ggtgcgcgcg gcgatcttct cgcggagggg aggatgccct tccgtggcgg
     2461 agtactgaag cgcccgtctg ccgttggcgt cgagcacctt cacggccacc ttcttcaaat
     2521 cctcgaccgg gaagagctcc ggcgccggaa ggcctccggc gaacgagatc acttcaggat
     2581 ttgctgcaag acgcaggatg ggcgtcagat cggactcttg aaagcgcgtc attcttcgtg
     2641 caaactgtgg attcatagca atgcctcctg gacgaaagat tggagaggat tataccccga
     2701 agcgtttcgt ccgaaaagat ggccttttca atcgaaagtg aggaacctga gaaaattccc
     2761 cccggaataa gcgcacaacg aatgctcagc gggaagccgc ttcacgaaga aagagttcgt
     2821 accggtcgag cagatcgtgc agttcacgaa ggcgcggatc gtccgttttc aaaccctccg
     2881 tcctggcatc aatctcaaga acgcggaata atgagatgtc ctctcctccc atgagatagc
     2941 caagtactcc cttgagttta tgcaacgcgc gcgctgctgc ggaggaatcg cccttttcca
     3001 gggagctccg aagcgagccg cgcagctccg ggacttcgga aaagaaggcc tccaccatct
     3061 ccgccaacgc ttcccggtct ccgtcgagag agaggagcag ttcggcgaac ccgctctccc
     3121 agcgctcccg ctctccattt tcggcagaaa accgcgcggg cgtttcttcg ctgtcggtgt
     3181 gcagagaggg agagggtgtc ccgagaaatc tggcgagcaa cgagtacagc ttgcgggacc
     3241 gaatgggctt ggagatatac tccgtcatgc ccgcctccag gcatcgctcc cggtcccctt
     3301 tcatcgcata cgcggtgagc gctacaatag gaatactctt tccgtcctgt tcttcggagc
     3361 gaatcctcct cgtcgcctcc aatccatcca tcaccggcat ctcgcaatcc atgagaatca
     3421 gatcgaacgg ttcgctgcgc cacatctcga ccgcctcccg gccgttttga gcgtgccgta
     3481 ccgtgagtcc tgtttttttg agcatcgcga cggtcatctt cgtattgacg gcgttatcct
     3541 cggcaagaag aacgttaccc ttcatcgatg agaaatccgt ttccgtaccc tcatgttcct
     3601 caacaatctc tccggagcag cgaagaaggg gaagagaaaa gcggaagacg ctccccctgc
     3661 cgacttcgct ctccacagtg atatcgccgc ccatcaggcg ggcgaggctg cgcgatatcg
     3721 caaggccgag cccggtccct ccgtgttttc gggtcgaaga accatccaac tgggtaaaat
     3781 cgtcaaacag aagatgcact ttgctttgcg gaatccccgg gccggtgtcg ctgaccgcaa
     3841 acgcgacact gatgcggtgc tcatcctctt cttcg
//
LOCUS       DAOTIL010000164         3874 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminobacteriaceae bacterium isolate AMR_MDS_3026
            NODE_27812_length_3874_cov_3.909400, whole genome shotgun sequence.
ACCESSION   DAOTIL010000164 DAOTIL010000000
VERSION     DAOTIL010000164.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868050
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminobacteriaceae bacterium (wastewater metagenome)
  ORGANISM  Aminobacteriaceae bacterium
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae.
REFERENCE   1  (bases 1 to 3874)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3874)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:19:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,224
            CDSs (total)                      :: 3,198
            Genes (coding)                    :: 3,184
            CDSs (with protein)               :: 3,184
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3874
                     /organism="Aminobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_27812_length_3874_cov_3.909400"
                     /isolate="AMR_MDS_3026"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:3034609"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            201..1211
                     /locus_tag="P5201_04610"
     CDS             201..1211
                     /locus_tag="P5201_04610"
                     /inference="COORDINATES: protein motif:HMM:TIGR02122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TAXI family TRAP transporter solute-binding
                     subunit"
                     /protein_id="HRV97847.1"
                     /translation="MKKVLIAVLVLFALCAAIGVMTGEAEAKSYRINIATATTGGAYY
                     PIGNAMAQIWSRNLEDVRRASAQSTAGTPQNVELMKDGEVQIAFGQNGICYYAYYGKG
                     IYDGKPPYTDLRGMFTLYPNVMHWLVRKGSGIQSIADFKERKFVPGQVASATEINSRE
                     MMEAYGLNYMKDAGEVNVTADFVGYNEAADLLKNAQTDGTHIAGGLPTAAIMDALSSG
                     VAELISIEEDKIKEICEKYPWYFPFTIPAGTYPNQEKEIKTLALANILFTDAKQDEEL
                     IYMLTKATYDYHEDLVNAHRATAYTVLENSLKGMTVPLHPGSVKYFKEMGVDVPEQLI
                     VK"
     gene            1277..3229
                     /locus_tag="P5201_04615"
     CDS             1277..3229
                     /locus_tag="P5201_04615"
                     /inference="COORDINATES: protein motif:HMM:TIGR02123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter permease"
                     /protein_id="HRV97848.1"
                     /translation="MTQQNDLLEQLPELDAERAAEKVLEKADAEARFRKYGGRWAVAV
                     TVIAVAMSIFHLYTSGFGTLMAMKQRSVHLAFLVLLTFLLYPGSKRAPRNRPSVVDWI
                     WIILSVSSSLYIFFTFEAFSMRGTAIPLDITMGAILIVCVLEATRRSVGKELMILALL
                     FLAYGYWGEYIPGVLGHTGFTYKRIVYQLFLSSEGIFGISLGVSATYIFLFILFGAFL
                     TETGMGRFIKDLAMSLAGRTIGGEAKVSIVASGLMGMINGSAVGNVAATGTFTIPLMT
                     GAGFKPVFAAAVVAVAGTGGMIMPPVMWAASFIMAEFLGLHYGVIMLAAAIPAVLYYL
                     AEYAVIHIEAVKMGMKPIPRDEIIPLTKVMRERGHLLVPIIVIVYLLLIGRTPLYAAF
                     YGIISSIAVSFLTKATRLTWKSFFKAMESGARQSVGVGIACAVVGNIIGITNLTGLGL
                     VLGDNIVSLAGGSVLITTFLTMIVCVILGMGLPTTACYIVTATIAAPALMKLGVNPLA
                     AHMFTYYFACLSNLTPPVAIASYGAAGLSGQSPSAVGWTGFRISLAGFLIPFTFIFNP
                     ELLLVGGDYVSITISTITSIIGVIALASSLQNFLYVRMNLIQRAGMFFGALLLIFPGL
                     TTDIAGVALTGTVFFWQRMQARSRAL"
     gene            3260..>3874
                     /locus_tag="P5201_04620"
     CDS             3260..>3874
                     /locus_tag="P5201_04620"
                     /inference="COORDINATES: protein motif:HMM:NF021387.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050043 - lactate racemase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lactate racemase domain-containing protein"
                     /protein_id="HRV97849.1"
                     /translation="MKTKMKSGKEEIFLSIPDKNVIAVLEPSGGSPVGDLGEEVRRLL
                     AAPTAGPSLEEIVLKGPARSVAVIVNDMTRSTPTASMLPPLLEELERLGVGREKVTVV
                     VATGTHRPMTDDEIRVVLGDAVFGTVKVENHDCDSPDLVDMGILSTGNRLLINKTVAE
                     ADLRIAIGEVLLHYYAGFAGGRKSILPGVAGRETVMNNHKMMTEP"
BASE COUNT          773 a         1120 c         1147 g          834 t
ORIGIN      
        1 tggaggaaat ccgtacgctg tctctggagg ctccggtgga gatagggagc gtggtgctcg
       61 cgtcccccgc cggctgtgat accgaggtcg ttgcgacccg gagagtgaag gctgcggccc
      121 cctgcgccgg ggcgtaaggc agggtgcgca tgcccgaact gaatggcgaa gaagttatac
      181 atcagaagga gggaatcgtt gtgaaaaaag ttcttattgc agttcttgtc ctgttcgctt
      241 tgtgcgcggc aattggagtc atgaccggag aggccgaggc gaaatcgtac cgcatcaaca
      301 tcgcgaccgc cactaccggc ggagcctact accccatcgg gaacgcgatg gcgcagatct
      361 ggtccagaaa ccttgaagac gtccgcaggg cgtccgcaca gtctacagcg ggcactccgc
      421 agaatgtcga acttatgaag gacggcgaag tgcagatcgc cttcggacag aacggtatct
      481 gctactatgc atactacggc aagggaatct acgacggtaa gcccccgtac accgacctcc
      541 ggggtatgtt cacgctctac cccaacgtaa tgcactggct cgtccggaag gggtccggga
      601 ttcagtcgat cgccgatttt aaggagagaa agttcgttcc cggacaggtt gcaagcgcaa
      661 cggagatcaa cagccgggag atgatggaag cctacggcct gaactatatg aaggacgcgg
      721 gagaagtgaa cgtaaccgcc gacttcgtcg gatacaacga agccgccgac cttctgaaga
      781 acgcccagac cgacggaacg catattgccg gcggccttcc cacagccgcg atcatggatg
      841 cgctctcctc cggagtagcc gagctgatct ccatcgaaga ggacaagatc aaagaaatct
      901 gcgagaaata cccctggtac ttccccttca caattcctgc gggtacctat ccgaatcagg
      961 aaaaggaaat caagacgctt gctcttgcga acatcctttt cacagacgcc aagcaggatg
     1021 aagagcttat ctacatgctc accaaggcga cgtacgacta ccacgaggat cttgtgaacg
     1081 cgcacagggc gaccgcctac accgttctcg agaactcctt gaaaggaatg accgttccgc
     1141 tgcatccggg ctctgtgaag tatttcaaag agatgggcgt cgacgttcct gagcagctta
     1201 ttgtgaaata gcaaccgatt gaaagcgggg aggaggctcc gccttctccc catctgtgta
     1261 ttcggaggta gagagtgtga cacaacaaaa cgatcttctt gagcaactcc ctgaactcga
     1321 cgcggagcgc gctgccgaaa aggtcctcga aaaggccgac gccgaagcgc gcttccggaa
     1381 gtacggcggc cgctgggccg tcgcggtgac tgttatcgct gtagccatgt ccatcttcca
     1441 tttgtacacc tccggtttcg ggacgttgat ggcgatgaag cagcgcagcg tccaccttgc
     1501 cttcctggtg ctcctcacat ttcttctgta tccggggtcg aaacgagcgc ctcgcaacag
     1561 gccgtccgtg gtggactgga tctggattat tctctcggtt tcatcgagtc tctatatttt
     1621 ctttacattc gaggccttca gtatgcgtgg aaccgccatc cctctggata tcacgatggg
     1681 agcgatcctc atcgtctgcg tccttgaggc gacaaggcgt tccgtgggca aggagctgat
     1741 gatcctcgcg ctgctctttc tcgcgtacgg ctactggggc gaatacatcc ccggcgttct
     1801 tggacacacg gggttcacct acaaacggat cgtctaccag ctgtttcttt cttccgaggg
     1861 tatcttcggc atctccctcg gcgtgtcggc aacgtatatt ttcctgttca ttctcttcgg
     1921 cgctttcctg accgagacgg gaatgggccg tttcatcaag gatctggcga tgtcgcttgc
     1981 cggccgcacc atcggcggcg aggcaaaggt ctccatcgta gccagcggcc ttatgggaat
     2041 gatcaacggc agcgcggtgg ggaacgttgc cgccacgggg acgttcacca tccctctgat
     2101 gaccggggcc gggttcaagc cggtcttcgc ggcggcagtt gtggcggtcg ccgggacggg
     2161 cggaatgatc atgcctccgg tcatgtgggc tgcgtcgttc atcatggcgg agttcttggg
     2221 gctgcactac ggcgtcatca tgctcgcagc cgccattccg gcggtgctct actatctggc
     2281 cgagtacgcc gtcatccata tcgaggccgt caagatgggg atgaagccta tccccagaga
     2341 tgagatcatt cccctgacga aggtcatgag ggagcggggg caccttctcg tccccatcat
     2401 agtgatcgtc taccttctgc tcatcggacg gacgccgctc tacgcagcgt tttacggcat
     2461 tatctcctcc atcgccgtca gcttcctgac gaaggcgacc cgccttacct ggaaatcctt
     2521 cttcaaggcg atggaatccg gagccaggca gagcgtcggc gtcggtatcg cctgcgccgt
     2581 cgttggcaat atcatcggca ttacgaacct gacggggctg gggcttgtgc tcggcgacaa
     2641 catcgtctcg ctcgcgggcg gcagcgtcct catcacgacc ttcctcacga tgatcgtctg
     2701 cgtcatcctc ggcatggggt tgccgaccac cgcgtgctac atcgttaccg cgaccatagc
     2761 ggctcccgcg ctgatgaagc tgggcgtcaa tcctctggct gcgcacatgt tcacctacta
     2821 cttcgcctgt ctctcgaacc tgacgcctcc ggtggccatc gcctcctacg gggccgcggg
     2881 actttcgggg cagagcccct ccgccgtggg ctggacgggt ttcagaattt cgcttgccgg
     2941 ttttcttatc cccttcacgt tcatcttcaa ccctgaactt ctgctcgtgg ggggcgatta
     3001 tgtctccatc accatctcga cgattacgag catcatcggg gttatcgcgc tcgcttcgtc
     3061 gctgcagaat ttcctgtatg tccggatgaa tttgatccaa cgggcgggta tgttcttcgg
     3121 cgcgttgctg cttatcttcc cgggattgac cacggatatc gccggagtag cgctgacagg
     3181 aacagttttc ttctggcaga ggatgcaggc tcgttcgcgc gccctctgaa gcttggtgag
     3241 atgagggagg gtgtacggaa tgaaaacaaa gatgaaatcg ggcaaggaag aaatatttct
     3301 ctccatcccc gataagaacg tcatcgcggt ccttgaacct tcgggcggga gtccggtcgg
     3361 ggatctcggg gaagaggtgc gcaggctgct tgccgcgccg accgcggggc cttctcttga
     3421 ggagattgtc ctgaaaggtc cggcgcgatc cgtggcggtg attgtcaacg acatgacccg
     3481 ttccacgccg actgcttcga tgcttccgcc gctccttgaa gagcttgagc gtctcggcgt
     3541 cgggcgcgag aaggtgacgg tcgtcgtcgc caccgggacg caccggccga tgaccgacga
     3601 tgagattcgc gtcgttctcg gcgacgccgt cttcgggacg gtgaaggtgg aaaatcacga
     3661 ctgcgactcc ccggacctgg tggatatggg aatcctctcc accgggaacc ggctgctcat
     3721 taataaaacc gttgcggaag cggatctgag gatcgccatc ggagaggtgc tgctgcacta
     3781 ctacgccgga tttgccggcg gtcggaagag catccttccc ggagttgcag ggcgcgagac
     3841 ggtgatgaac aaccacaaga tgatgaccga gccg
//
LOCUS       DAOTIL010000165         3867 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminobacteriaceae bacterium isolate AMR_MDS_3026
            NODE_27875_length_3867_cov_4.025971, whole genome shotgun sequence.
ACCESSION   DAOTIL010000165 DAOTIL010000000
VERSION     DAOTIL010000165.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868050
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminobacteriaceae bacterium (wastewater metagenome)
  ORGANISM  Aminobacteriaceae bacterium
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae.
REFERENCE   1  (bases 1 to 3867)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3867)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:19:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,224
            CDSs (total)                      :: 3,198
            Genes (coding)                    :: 3,184
            CDSs (with protein)               :: 3,184
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3867
                     /organism="Aminobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_27875_length_3867_cov_4.025971"
                     /isolate="AMR_MDS_3026"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:3034609"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..761
                     /locus_tag="P5201_04625"
     CDS             <1..761
                     /locus_tag="P5201_04625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012868780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_component: GO:0009346 - ATP-independent citrate lyase
                     complex [Evidence IEA];
                     GO_function: GO:0008814 - citrate CoA-transferase activity
                     [Evidence IEA];
                     GO_process: GO:0006084 - acetyl-CoA metabolic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="citrate lyase subunit alpha"
                     /protein_id="HRV97850.1"
                     /translation="AFGLLKASGMLKNGCSFQTGGGGPSLAVARYVKEYMKSHAIKGS
                     YCIGGITGYMASMLEEGLFDALFDVQSFDAAVTSSIANNPRHIEVDVSCYANPFNKGC
                     LVNDLDVVILAALDVDTDFNVDVLTGHDGVLRGASGGHSDTAAGAKLTVITIPSMRNG
                     VPSIRNRVQTVVTPGETVDVIVTERGMAINPLREDLRALATDAGLPVKDIKQLKAEIE
                     KVTGIPEEVRFGDEIVGLVEYRDGTIIDTIRKVL"
     gene            complement(898..1689)
                     /locus_tag="P5201_04630"
     CDS             complement(898..1689)
                     /locus_tag="P5201_04630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009202087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HRV97851.1"
                     /translation="MHFGLEGKTVLVLASGSGLGKAVAGEFLKEGADVVLAGRGVEKL
                     ERAAADLRGATGITPRFQQCDITKAEELHGLVRFAAQKHGSVDVLVNNAGGPPAGTFE
                     IFDDAAWTGAFELTLLSYVRAIREVLPYMKQRGWGRIVNSTSSSVREAIENLILSNTF
                     RLGVIGLTKSLAAEFAPYGILVNAIGPGRFDTERIRSLDRSLAEKKGLSVEEVSRASL
                     SRIPMGRYGDPEEYGRLAVFLGSEANTYITGQTILADGGMTRAVP"
     gene            1871..3658
                     /locus_tag="P5201_04635"
     CDS             1871..3658
                     /locus_tag="P5201_04635"
                     /inference="COORDINATES: protein motif:HMM:NF012245.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing methyl-accepting
                     chemotaxis protein"
                     /protein_id="HRV97852.1"
                     /translation="MKSLTLKTRLWILALIPVFGILSVTGVLMYSANAIYNDLLAQIH
                     QEAFVAQSLVLNGDRDLYQGLVAKQSILQGGAGKDFDKNLKDFRENVAQVTERLGNAE
                     KLLARNKAAWEPFRLEGKTESVFDKFNTLNKDFPAWIKESEQQLDDIRTGKLAHGSVQ
                     QIEDPLFKKVRGNINEIGEILDIGAENSALANKQRMTAANISMGAVAIFVALLAVVIA
                     FTTIRKIRRSVASILAASKAGKEGNLTVRTSMTGDDELAAVGHSLDAMLDSLREVISE
                     LQKKSVALSTLSENTAASCEEVTSTTNEVAESNSKLAEEVSLGRSSAVDASRNILDMN
                     TVIQSAQAVASRANENSREMAEAAAKGRETVASSIGHMEGIRDAVADTGKTISQLNQY
                     SQRIGVVGTTITSLADQTNLLALNAAIEAARAGEAGRGFAVVADEVRKLAEQSQQGAR
                     EVAELVERILRGTSDAVTSMEKSLHDVEEGVAIAHVAGDALEKIMTATKSSVDDIRLI
                     IEATEQEADKAEVVVALIDRTSSVMENADMQVQTVAASMEETAAAMENVASGATEVNS
                     TAEDLRRLAERFIVDEESGGQLALPGRKS"
BASE COUNT          828 a         1203 c         1172 g          664 t
ORIGIN      
        1 tggcgttcgg gctcctcaag gcatccggga tgctgaaaaa cggctgctcc ttccagaccg
       61 gcggcggcgg cccaagcctc gctgtggcgc gctacgtgaa ggagtacatg aaatcgcacg
      121 caatcaaagg gagctactgc atcggcggca tcaccggcta tatggcgtcc atgctggaag
      181 aggggctctt cgacgctctc ttcgacgtgc agagcttcga cgcggccgtg acctcgtcga
      241 tcgcgaacaa cccgcggcac atcgaagtgg acgtctcctg ctacgccaac cccttcaaca
      301 aagggtgcct tgtgaacgac ctcgacgtgg tcattctcgc cgcgctcgac gtggacacgg
      361 acttcaacgt cgacgtgctc acagggcacg acggcgtcct caggggcgcg tccggcgggc
      421 actccgatac ggccgccggt gcgaagctca ccgtgatcac catcccctcg atgcgcaacg
      481 gcgtgccctc gatacggaac cgtgtgcaga cggtcgtgac gcctggagag actgtggacg
      541 tcatcgtcac cgaacgggga atggccatca acccgctccg ggaggacctt cgggctctcg
      601 ccacggacgc ggggcttccc gtgaaggaca taaagcagct gaaagcggag atcgagaagg
      661 tcaccggcat ccccgaagag gtccgcttcg gcgacgagat cgtgggcctt gtggagtacc
      721 gggacggcac gatcatcgac acgatacgga aagtgctgta gcccgcagaa aaaggtactt
      781 tctcaacaat caaaaggccg ggggaaatcg ccgcacgcgg cgaaggcccc tcggcctttc
      841 ctgcttcttt caactgaggc cccggttcgg caaaggggac tgtctctgtt gccgcctcta
      901 cggcaccgcc ctcgtcatgc cgccgtcggc gaggatcgtc tgcccggtga tgtaggtgtt
      961 cgcctccgac ccgaggaaaa cggcgagccg tccgtattcc tcggggtcgc cgtaccgtcc
     1021 catcggaatt cgcgagagcg acgctctgct tacctcctcg acggacagtc ccttcttctc
     1081 ggccagcgag cggtcgagcg accggatgcg ctccgtgtcg aagcgccccg ggccgatggc
     1141 gttcacgagg atgccgtaag gggcgaactc ggcggcgagg ctcttcgtca gcccgatcac
     1201 accgagccgg aacgtattgg agagaatgag gttctcgatg gcctcccgca ccgaagagga
     1261 cgtcgagttg acgatgcggc cccaaccgcg ctgtttcata tacggaagca cctcgcggat
     1321 cgcccgcacg tagctcagca gcgtcagctc gaaagctccc gtccaggctg cgtcgtcgaa
     1381 tatctcgaac gttccggcgg gaggccctcc cgcattgttc accagcacgt ccacgctgcc
     1441 gtgcttctgc gcggcgaagc gcaccaaccc gtgcagctcc tcggccttcg tgatgtcgca
     1501 ctgctgaaaa cgaggcgtga ttccggtcgc cccgcgcaag tcggccgccg ctctttcgag
     1561 cttctccact ccccttccgg cgagcaccac gtccgctccc tccttcagga actctccggc
     1621 cacggccttc ccgagcccgg aacccgaggc caaaacaagc acagtctttc cttccagtcc
     1681 gaagtgcatc agtcacccct ccttcgaata ctgctctcaa aaactatttc ttattttaca
     1741 ccttcttcgc ggacatctca cggcgtttac cccctggggg ctatccggaa tccccgagtc
     1801 cggtgtagaa tataaaaaat cctgtaccat actgtgaaaa aagtgccctc actgaaagga
     1861 gcggagaata atgaagagct tgacgttgaa aacgagatta tggattctcg cgcttatccc
     1921 ggttttcggc atacttagtg tgacaggcgt cttgatgtac tcggcgaacg ccatctacaa
     1981 cgaccttctc gcccagattc accaggaagc cttcgtggcg cagagcctgg tgctcaacgg
     2041 ggaccgcgac ctgtaccagg ggctcgtggc gaagcaatcc atactgcaag ggggcgccgg
     2101 aaaggacttt gacaaaaacc tcaaggattt ccgggagaac gtggcgcagg tcacggagcg
     2161 gcttggcaac gccgaaaaac tccttgcacg caacaaggcc gcgtgggaac cgttccggct
     2221 tgaggggaaa acggaatccg tcttcgacaa gttcaatacg ctgaacaagg acttcccggc
     2281 gtggattaaa gagtcggaac agcagctcga cgatatccgg acgggaaagc ttgcgcacgg
     2341 ctccgtgcag cagatcgagg atcctctgtt caaaaaagtc cgggggaaca tcaacgagat
     2401 cggcgaaatc ctggacatcg gcgccgaaaa cagcgcgctg gcgaacaaac agcgtatgac
     2461 ggcggcgaat atctccatgg gcgcggtggc gattttcgtc gcgcttttgg cagttgtcat
     2521 cgcgttcacc accatccgga agatacgccg ttcggtcgct tccattctcg cggcatcgaa
     2581 ggccggtaag gaggggaatc tgacagtccg gacctccatg acgggagacg acgaacttgc
     2641 ggccgttggc cattcgctcg acgccatgct cgacagcctc cgcgaggtta tctccgagct
     2701 tcagaagaaa tcggtcgccc tttccacgct ctcggagaac acggccgctt cctgcgagga
     2761 ggtcaccagc accaccaacg aggtcgcgga gagcaacagc aagcttgccg aagaggtcag
     2821 tctggggcgc agcagcgccg tcgacgcctc gcgcaacatc ctcgatatga acaccgtcat
     2881 ccagtccgca caagcggtcg cctcgcgggc caacgaaaac tcccgcgaaa tggcggaagc
     2941 ggcggcgaag ggaagggaga cggtcgcctc ctccatcggt cacatggagg gaatccggga
     3001 tgccgtcgcg gacacaggga agaccatctc gcagctcaac cagtattctc agagaatcgg
     3061 cgtcgtgggt acgaccatca cctcgctcgc ggaccagacc aacctcctcg cgctgaacgc
     3121 ggccatcgag gctgcccgag cgggcgaggc cggacgagga ttcgccgtcg tcgccgacga
     3181 ggtgcgcaag ctcgccgagc agtcgcagca gggagcgcgg gaagttgcgg agcttgtgga
     3241 acggatcctc cggggaacct ccgacgcggt gacctccatg gagaagagcc tgcacgacgt
     3301 cgaagagggg gtcgccattg cgcacgtcgc gggagacgcg ctggagaaga tcatgacggc
     3361 gacgaaaagc tccgtggacg acatccggct gatcatcgaa gccacggaac aggaggccga
     3421 caaggcggaa gtcgtcgtcg cgttgatcga cagaacatcg tcggtgatgg agaacgcgga
     3481 tatgcaggta cagaccgtgg ctgcctccat ggaagagact gcggcggcca tggagaacgt
     3541 cgcgagcggc gccaccgagg taaacagcac cgcggaggac ctgcgcaggc ttgcggagcg
     3601 attcatcgtc gacgaagaaa gcggcgggca gctcgccctt cccgggagaa aatcctgacg
     3661 gaaaaagcgt acacgcccga tgagtttgac aaacgccccc tcgctgcgag ggggcgtttg
     3721 tgtacctctt tacagaactc tgataaacgc gggttcagag atccgcttcg ccgctctcag
     3781 tccagctcag cgatggcgtc gatctccacc agcataccaa agagctggct ctgcacagtg
     3841 atccgcacag ggtacggttc ggtaaaa
//
LOCUS       DAOTIL010000166         3865 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminobacteriaceae bacterium isolate AMR_MDS_3026
            NODE_27904_length_3865_cov_4.220735, whole genome shotgun sequence.
ACCESSION   DAOTIL010000166 DAOTIL010000000
VERSION     DAOTIL010000166.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868050
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminobacteriaceae bacterium (wastewater metagenome)
  ORGANISM  Aminobacteriaceae bacterium
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae.
REFERENCE   1  (bases 1 to 3865)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3865)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:19:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,224
            CDSs (total)                      :: 3,198
            Genes (coding)                    :: 3,184
            CDSs (with protein)               :: 3,184
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3865
                     /organism="Aminobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_27904_length_3865_cov_4.220735"
                     /isolate="AMR_MDS_3026"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:3034609"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            172..681
                     /locus_tag="P5201_04640"
     CDS             172..681
                     /locus_tag="P5201_04640"
                     /inference="COORDINATES: protein motif:HMM:NF013731.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheW"
                     /protein_id="HRV97853.1"
                     /translation="MTEKKEAFYLIFRVGERRFAIAAAEIVRVLAAVEVTPLPDTPSF
                     LRGVINCGGVLVPVIDLRVRFGLPVRRVRPSDRLIFVRNAASAFQKDGRLCSFLVEDV
                     EGVFPLAPETIALPESSSGLFNAALAGLESGDDRVVCIQTADTLFSMTEAEILQVERA
                     SPGTEDILL"
     gene            678..2150
                     /locus_tag="P5201_04645"
     CDS             678..2150
                     /locus_tag="P5201_04645"
                     /inference="COORDINATES: protein motif:HMM:NF013867.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CheR family methyltransferase"
                     /protein_id="HRV97854.1"
                     /translation="MTQSSRFILSPEAEQDLRRLGLLVEKRLGLVFSEKRSRDLWQAM
                     LSLAAFAQRSPTELMHKWANEGLSEAATTALAEYLTIGETYFFREPKTLRAFEELVLK
                     RFTSGKKPERFRIWCAGCSTGEEAYTLSIILHRLLPDIRSMDVSILGTDINPRVLEKA
                     RRGAYSSWSFRGLEKSLKERFFEPLDDGSWSVKHLFRQRVSFSLLNLADDEWRLWDDG
                     SHPDVLFCRNVLIYFSADQRKNVVFRFHSLLPPEGWLVVAPCESSALLASKFTPVHHD
                     GATLYKKQAVPEPTERDVSFPEPLFSALPVGAMLDEAPMEEEPAETGVPYEEPPFLAA
                     ETFLGTVPEYQEDEEPGEALSESVGACLQNARILADKGRHDEALQWALKAKDLDRTAP
                     EPLYLAALISSETGDEREAAASLRSALFLAPDFVMAHYALASLAMKSGKDADSERHLR
                     NAEALLSSLPPDAPLREGDGLCAGDLLQTVRGLRRNSIFE"
     gene            2169..2714
                     /locus_tag="P5201_04650"
     CDS             2169..2714
                     /locus_tag="P5201_04650"
                     /inference="COORDINATES: protein motif:HMM:NF013731.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheW"
                     /protein_id="HRV97855.1"
                     /translation="MEISADALDQERMDAVLRKRAKKLSREFLKESADEEDLLEIVEF
                     RLGQERYGVASLHVGEVIPLRERAVLPCTPPFVAGILNLRGRIVSLIDLKFFFDIPAT
                     EISKETRVIVLRSGEMELGILADALVGIRMISLRNIHPPLPTMAGVRSAYLLGVEGED
                     TALLDGGRILKDESLIVNEEV"
     gene            2733..>3865
                     /locus_tag="P5201_04655"
     CDS             2733..>3865
                     /locus_tag="P5201_04655"
                     /inference="COORDINATES: protein motif:HMM:NF024140.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HRV97856.1"
                     /translation="MVKLRNVSIGKRLGFGFGTVVLIALALGITGILGISRLSNDLEY
                     ITTNRISDLRSLAGLNYQRMAIRAETLGVVITEDKSNRDEELLKILERRKKASDRIDE
                     HWNALLSVPRHMEKGRQLVEILKVQYQSWRKIHGDIDRLVEEILRSQSVDRKNTLFQE
                     LKAQVYRSIPISDVMGSTFDDLTRNNNEQTDLLVEGDLKRAGMMEMFFLLAMIVGVFI
                     ALVMTVKISRSIVLPIDSAVSFLSRLREGELRENVPQEFLMRKDEIGTLAQAVQDLTE
                     ELREQIASMKEVTVALASSAYQISASVSEVTAGAEETAVAVVETTATMEEVRTTAEVT
                     NRKSKEVAESAQQGLQVVQKGKLATDALFDGMKNVGERMTSIA"
BASE COUNT          841 a          994 c         1140 g          890 t
ORIGIN      
        1 ccagtgtgtg ataaacattg tacttgcgag catacacgcc acgtttttcc ctgtcaagac
       61 cgctttcctc cttttcccaa agactgctat aatttctcag acaggagttc tttctccagt
      121 cgcctggagg acgagcaggt attcggatca agggaaggct gggagaaaga gatgacggag
      181 aaaaaagagg ctttctatct tattttcaga gtaggagaaa gacggtttgc cattgcggcg
      241 gcggagatcg tgcgagtgct tgcggcagtg gaagtcacac ccttgccgga tactccgtcg
      301 ttccttcgcg gtgtgattaa ctgcggtggc gtacttgttc ccgtgattga tctccgggtg
      361 cgtttcgggc ttcctgtcag aagagtgcgc ccgagcgacc gtctcatctt tgtccggaac
      421 gcagcttccg catttcaaaa ggatgggcgc ctttgctcgt ttctcgtgga agatgtggaa
      481 ggagtgttcc ctctcgcgcc tgaaacgatc gctctgcccg aatcctccag cggtcttttc
      541 aatgcggcgc ttgccgggct cgaaagcggc gatgaccgcg tcgtgtgcat tcagacggca
      601 gatactctct tttctatgac cgaagcggag attctgcaag tcgagagggc ctcccccggt
      661 acggaggaca tccttttatg acgcagtcga gccgttttat tctttctccg gaggcggagc
      721 aggatcttcg gcgcctcggt cttttggtag aaaagcgtct tgggctcgta ttttctgaaa
      781 aacgatctcg tgatctctgg caggcaatgc tctcccttgc cgccttcgca cagcggtctc
      841 cgaccgagct catgcacaag tgggcgaacg aggggctttc agaggccgcc actacggcgc
      901 tggctgagta tcttacgatc ggagagacct atttctttcg ggagccgaaa acactgcgcg
      961 catttgaaga actggttctg aagcgtttca catccgggaa gaaaccggag cgtttcagga
     1021 tatggtgcgc cggatgcagt acgggagaag aggcgtatac gctctctatt attttgcacc
     1081 gattgcttcc ggatattcgc tcaatggacg tttcgatcct ggggaccgat atcaatcctc
     1141 gagtcctcga aaaggcccgg cggggagcgt actcttcctg gtccttcaga gggcttgaaa
     1201 agtctctgaa agagaggttt ttcgagcctc ttgacgacgg gagctggtct gtaaagcatc
     1261 tctttcgcca acgagtttct ttttccctcc tcaatctagc cgacgacgaa tggcgtctct
     1321 gggacgacgg ctcgcacccc gatgttctct tctgccggaa tgtgctgatc tatttttccg
     1381 ccgatcagcg caagaacgtc gtctttcgtt tccactcgct tcttcccccc gaaggctggc
     1441 tcgtggttgc cccgtgcgaa tcctcggctt tacttgcgtc gaagtttacc cctgtgcatc
     1501 atgacggggc gaccctttac aaaaaacagg ctgtcccgga gccgacggaa agagacgtct
     1561 ctttcccgga acctcttttt tcggctcttc ctgtaggagc gatgctcgac gaggctccga
     1621 tggaggagga acccgcagag acgggggttc cttacgaaga acctccgttc cttgccgctg
     1681 aaacctttct tggaactgtt ccggagtatc aggaggatga agaacccggg gaagcgcttt
     1741 ccgaatccgt cggcgcatgc ctgcaaaacg cccggattct cgccgacaag ggaagacatg
     1801 acgaggcgct ccaatgggcg ctgaaagcca aagatctgga ccggacagcc cctgaaccgc
     1861 tttatctggc tgccctgatc agctccgaga cgggagacga aagagaggct gcggcatcgc
     1921 tccggagcgc cctctttctt gctcctgatt tcgtcatggc gcactatgct ctcgccagcc
     1981 ttgcgatgaa aagcggcaag gatgccgatt cggaacgtca tcttcggaac gcggaggcgc
     2041 ttctctcttc tcttccgccg gacgctccgc tgcgggaagg cgacggactc tgtgcgggag
     2101 acctcctgca gaccgttcgc gggctgcggc gaaactccat ttttgaatag agggtggtga
     2161 gacggagaat ggaaataagc gccgatgcgc ttgaccaaga acggatggac gccgtcctcc
     2221 ggaaaagagc caaaaagctt tccagggaat tcttgaagga gagcgcggat gaagaggatc
     2281 ttttggaaat cgtcgaattc cgtctagggc aggagaggta cggagtcgct tcgctgcacg
     2341 tcggtgaagt tatcccgttg cgtgaacgtg cggttctgcc atgcactccc ccttttgtgg
     2401 cgggaatact caatctccgg ggacgcatcg tttctttaat tgatctgaag ttttttttcg
     2461 acatcccggc gacggagatc tcaaaagaaa cgagggttat agtgctgcgt tcgggagaga
     2521 tggagctggg aattctggcc gacgctttgg tcgggatacg catgatctct ctccggaata
     2581 tccatccgcc gctgccaacg atggcgggcg tgcgctctgc atatctgctc ggagtggagg
     2641 gagaggacac cgctctgctt gacggaggaa gaatactcaa ggatgaatcg ctgatcgtca
     2701 acgaggaagt ttaaagcagt gaggaggacg acatggtgaa attacggaat gtaagcattg
     2761 gcaagagact cggtttcggt ttcggcacgg tcgtattgat tgcgttggcc cttgggatta
     2821 ccggtatact gggtatttca aggctctcga acgatcttga atacataacg acgaaccgca
     2881 tctccgattt gcgttcgctt gccgggttga actatcagcg gatggccatc agagcggaga
     2941 ctctgggcgt tgtaataacc gaagacaaat cgaaccggga tgaagagttg ctgaaaatcc
     3001 tggaacggcg gaaaaaagcc tcggatcgga tcgacgagca ctggaacgca cttctctcag
     3061 tgccgcgaca catggagaaa gggagacagc ttgtagaaat tctgaaggtt cagtatcagt
     3121 cctggcgcaa aattcacggg gatatcgaca ggcttgtgga ggagatcctc cgaagccagt
     3181 cggtcgacag aaagaatacg ctttttcagg agttaaaggc ccaggtatac cggtcgattc
     3241 ctatctcgga tgtcatgggc tccacttttg atgatctcac ccggaacaac aacgaacaga
     3301 ccgatcttct tgttgagggg gatttgaagc gcgcgggaat gatggagatg tttttcctcc
     3361 tggcgatgat cgtcggcgta ttcatcgcgc tcgttatgac ggtgaagata tcccggagta
     3421 tcgtccttcc gatagattcc gcggtctctt tcctctcccg ccttcgcgaa ggggaattgc
     3481 gtgaaaacgt cccgcaggag ttccttatga ggaaggacga aattggcacg ctggcgcaag
     3541 cagtccagga tttgacggag gagcttcggg agcagatcgc ctccatgaag gaagtgacgg
     3601 ttgccctggc gtcttccgca taccagattt ccgcctcggt ttccgaggtc accgcaggcg
     3661 ccgaggaaac cgcagtcgcg gtcgtggaga ccacggcgac catggaagag gtgcgcacca
     3721 ccgccgaggt gaccaacaga aagagcaagg aagtcgccga gagcgcgcag caggggttgc
     3781 aggtggtgca gaaggggaaa ttggcgaccg acgctctgtt cgatgggatg aaaaacgtcg
     3841 gagagcgcat gacctcgata gccga
//
LOCUS       DAOTIL010000167         3833 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminobacteriaceae bacterium isolate AMR_MDS_3026
            NODE_28192_length_3833_cov_3.303600, whole genome shotgun sequence.
ACCESSION   DAOTIL010000167 DAOTIL010000000
VERSION     DAOTIL010000167.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868050
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminobacteriaceae bacterium (wastewater metagenome)
  ORGANISM  Aminobacteriaceae bacterium
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae.
REFERENCE   1  (bases 1 to 3833)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3833)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:19:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,224
            CDSs (total)                      :: 3,198
            Genes (coding)                    :: 3,184
            CDSs (with protein)               :: 3,184
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3833
                     /organism="Aminobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_28192_length_3833_cov_3.303600"
                     /isolate="AMR_MDS_3026"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:3034609"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..770)
                     /locus_tag="P5201_04660"
     CDS             complement(<1..770)
                     /locus_tag="P5201_04660"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRV97857.1"
                     /translation="MKNMDNSGIPGKEERGGDRLCPECGSRVENSFRFCPGCGSFVPE
                     YASVRQVPGAQRKETEYEDDGEDDGVVSVACGPGGNEREVEGAFAYEQPRSNADRAGT
                     SFREFNAAIQDRRGRRDRKGGIFLLLLFFMFVAALGGGVYWFLQQAEQIPWENDVREK
                     PAADPTPVAQENRDPDLPPLATPGGAPADVTPALPLSPPVQADALEISRPTKGVVIGS
                     GVNLRGSHTVSSPVVGKVTTGNEVEVLESWTSDEGAEVV"
     gene            complement(900..2588)
                     /locus_tag="P5201_04665"
     CDS             complement(900..2588)
                     /locus_tag="P5201_04665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012870326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004819 - glutamine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006425 - glutaminyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--tRNA ligase/YqeY domain fusion
                     protein"
                     /protein_id="HRV97858.1"
                     /translation="METQSGKGPHFIEQIIMDDLKNGVVKEVHTRFPPEPNGYLHIGH
                     AKSICLNFGLAEQFGGKCNLRFDDTNPAKEETEYVESIMRDVQWLGFRWHALRFASDY
                     FQQFYEWALELIHAGKAYVDHQTPDEIRETRGTLTAPGVESPYRNRSVEENLALFQQM
                     KDGVLEDGTCVLRAKIDMNHPNINMRDPVLYRILHRHHFRTGDAWCIYPMYDFAHGYE
                     DAIEGVSHSICTLEFEDHRPLYEWFLDNVSVPCTPRQFEFARLNLTYTVMSKRLLLEL
                     VNRKIVSGWDDPRMPTISGIRRRGYTPSALRRFCAEIGVSKSNSMVDIEFLQFIIREE
                     LNATSLRAMAVLDPLKVVVENYPEGQVEMLDAENNPEDPNAGTRQIPFSRELYIERED
                     FMENPPKKFFRLSPGSEVRLKHAYFITCKEVVRNDDGEIVELRCEYDPLTRGGEAPDG
                     RRVKGTLHWVSVPHAVKAQVRLYENLFTLRDMSEMEEGKSYNDYLNPESLTVLENCFV
                     EPALAAAKPLDRFQFLRQGYFCADADSTPDRPVFNRTVALKDSWAKMQQKGQAD"
     gene            complement(2842..3441)
                     /locus_tag="P5201_04670"
     CDS             complement(2842..3441)
                     /locus_tag="P5201_04670"
                     /inference="COORDINATES: protein motif:HMM:NF013077.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HRV97859.1"
                     /translation="MTMNFFDAVKNRRTYYSISKKSPVSDERIREIVEQAVKHCPSAF
                     NSQSGRAVLLFGAESDAFWSLVKESLRKIVPAEKFAPTEEKIDGFASGYGTVLFFEDE
                     RPVKGLQESFPTYAEHFPVWSHHASGMLQFIVWAGLEAEGFGASLQHYAPVVEQAVKQ
                     RWGFPAEWKLIAQMPFGTPTAGPGDKEFQPLEERFKVFK"
BASE COUNT          749 a         1255 c         1058 g          771 t
ORIGIN      
        1 acgacttccg ccccctcgtc gctcgtccac gactccagta cctcgacctc gttgcccgtg
       61 gtcaccttcc ccacgacggg gctgctcacc gtgtgcgagc cgcgaaggtt cacaccggag
      121 ccgatgacta cccccttcgt cggacgggag atttcgaggg catccgcctg cacgggggga
      181 gacaagggca gtgcgggagt cacatcggcg ggcgcccctc cgggcgtcgc caacggcggc
      241 agatcaggat cgcggttctc ctgcgccaca ggcgtcgggt cagccgcggg cttttcacgc
      301 acgtcgttct cccagggaat ctgctccgcc tgctgaagga accagtagac gcctccgccg
      361 agcgccgcca cgaacataaa aaagaggaga agcaggaaaa tgccgccctt cctgtctctt
      421 ctgcctcggc gatcctgaat ggctgcgttg aactcacgaa aacttgtgcc tgccctgtcg
      481 gcgttggaac gaggctgctc gtacgcaaac gccccctcga cctccctctc gtttccgccc
      541 ggtccacacg ctacggagac gacgccatca tcttcaccat cgtcttcgta ctccgtttct
      601 ttgcgctgtg caccgggaac ctggcgtaca gaggcgtatt cagggacgaa cgagccgcat
      661 ccggggcaga agcgaaagct gttctccact ctgctcccgc attccggaca aaggcggtcg
      721 ccgccccgtt cttcttttcc gggaatcccg gagttgtcca tattcttcat gtacaggaaa
      781 cctcctctcg aatccacgga aaaacgcctt tacagagtat accgtgtacc atttctattc
      841 tatcatttcc caggactgcg tttcctccgt caaataccgg aacatggagg ctgaaagact
      901 cagtcggcct gccccttctg ctgcattttc gcccacgagt ccttcagcgc gaccgtcctg
      961 ttgaacaccg gccggtcagg cgtcgagtca gcgtccgcgc agaagtaccc ctggcggagg
     1021 aactggaagc ggtcaagcgg cttcgccgct gcgagcgccg gttcgacaaa gcagttctca
     1081 agcaccgtca gcgactccgg attcaggtag tcgttgtagc tcttcccctc ctccatctcg
     1141 ctcatatcgc gcagcgtgaa gaggttctcg tagagtcgca cctgcgcctt caccgcgtgc
     1201 ggaacggaga cccagtgcag cgtccccttc acgcggcggc cgtccggcgc ttcgcccccc
     1261 ctggtaagcg ggtcgtactc gcagcgcagc tcgacgatct ctccgtcatc gttccgcacg
     1321 acctccttgc acgtgatgaa gtacgcgtgc ttcagccgga cttcgctccc cggagagaga
     1381 cggaagaact tcttcggcgg gttctccatg aaatcctccc gctcgatgta gagctccctg
     1441 gaaaagggaa tctgcctcgt cccggcgttc ggatcctccg gattgttctc ggcgtcgagc
     1501 atctcgacct gcccctcggg gtagttctcg acgaccacct tcagcggatc gagcaccgcc
     1561 atcgcccgca gcgacgtcgc gttcagctcc tccctgatga tgaactgaag gaactcgata
     1621 tccaccatgc tgttcgactt cgacacgccg atctccgcgc agaaacgccg gagcgccgac
     1681 ggcgtgtacc cccttctccg gatgccgctg atcgtgggca tccgcggatc gtcccagccg
     1741 gagacgatct tccggttcac cagttccagc agaaggcgct tgctcatcac ggtgtacgtc
     1801 agattcaacc gggcgaactc gaactggcgg ggcgtgcacg gaaccgagac gttgtccagg
     1861 aaccactcgt acagcggacg atggtcctcg aactccagcg tgcagatcga atgggagact
     1921 ccctcgatgg cgtcctcgta gccgtgggcg aagtcgtaca tcgggtagat gcaccacgcg
     1981 tccccggtgc ggaagtgatg tctgtgcaga atccggtaga gcaccggatc gcgcatgttg
     2041 atgttcggat ggttcatgtc gatcttcgca cggaggacgc acgttccgtc ctcaagcacg
     2101 ccgtccttca tctgctggaa gagcgcgagg ttctcctcga cggaacggtt cctgtagggg
     2161 gactcgacgc cgggggccgt cagcgtgccc cgcgtctccc ggatctcgtc gggcgtctga
     2221 tggtccacgt aggccttccc cgcgtgaatc agttccagcg cccactcgta aaactgctga
     2281 aagtagtccg acgcgaagcg gagcgcgtgc cagcgaaacc cgagccactg gacgtcgcgc
     2341 atgatggact cgacgtactc cgtctcctcc ttggccggat tcgtgtcgtc gaagcgcagg
     2401 ttgcacttgc cgccgaactg ctcggccagc ccgaagttca ggcagatcga cttcgcgtgc
     2461 ccgatgtgaa ggtagccgtt gggctctggt ggaaagcggg tgtgcacttc cttgacgacg
     2521 ccgttcttca gatcgtccat gatgatctgt tcgatgaaat gtgggccttt gccggactga
     2581 gtctccatag gtcagaaaac gctcctttat actaacggat tgaagttcgc cgcaacatca
     2641 gcaaacatcg tgtgcttcat acaggcaaca gcgcgcccgt ttccgcggcg cacgcaccta
     2701 ttttcacacg gagagcccga aatgtaaaat tctccctccc tctacgggga aaaatccgta
     2761 gaaagaggga gatgaaggaa aaagggtgtt ccacgccctt gaggaatcgc ggaacaccct
     2821 tgaaagaacg attggaaaac gctacttgaa taccttgaaa cgctcctcca gcggctgaaa
     2881 ctccttgtcg ccggggcctg ccgtcggcgt tccgaagggc atctgcgcga tcagcttcca
     2941 ctcggcgggg aatccccacc gctgcttcac cgcctgctcg acaacaggcg cgtagtgctg
     3001 cagcgacgct ccgaacccct ccgcctccag ccccgcccag acgatgaact gaagcatccc
     3061 cgacgcgtgg tgcgaccaga cggggaagtg ctcggcatat gtggggaagc tctcctggag
     3121 ccccttcacc gggcgctcgt cctcgaagaa gagcaccgtg ccgtaccccg aggcgaagcc
     3181 gtcgatcttc tcctccgtcg gcgcaaactt ctccgccggg acgatcttcc ggagcgactc
     3241 cttcacaaga ctccagaacg cgtcgctctc cgccccgaaa agcagcacgg cacgcccgct
     3301 ctgggagttg aacgccgagg ggcagtgctt caccgcctgc tcgacaatct cgcgtatccg
     3361 ctcgtcggag accggactct tttttgaaat gctgtagtac gttctgcggt tcttcaccgc
     3421 gtcaaagaaa ttcatggtca ttgcacatac ctcctgcata tattcaaatt cggagaggat
     3481 gcttcagcct cccttgctgc cacatccgcc cggcggaacg ttttttaggc gaaccatccg
     3541 cctgttcgga tatgattttt ataaatttac atcgctttaa aagctattgt accatgaatc
     3601 catataaaat aaagccggca aaagaaatgc tcttcggtga aacaacaacg aagacatttc
     3661 accccgtcac cgcacagcgc acaatactgc ctcggcggcg gcacatgcgc cgcgcgggtt
     3721 gacgcaggct gggggcaccg gagggagatc ttcaagcgag cgtctcaacc gttcgccgcc
     3781 ccatgttccc cacgcgccga ggttcacacc caggccgcga cgctctatct cgc
//
LOCUS       DAOTIL010000168         3827 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminobacteriaceae bacterium isolate AMR_MDS_3026
            NODE_28257_length_3827_cov_2.730382, whole genome shotgun sequence.
ACCESSION   DAOTIL010000168 DAOTIL010000000
VERSION     DAOTIL010000168.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868050
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminobacteriaceae bacterium (wastewater metagenome)
  ORGANISM  Aminobacteriaceae bacterium
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae.
REFERENCE   1  (bases 1 to 3827)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3827)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:19:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,224
            CDSs (total)                      :: 3,198
            Genes (coding)                    :: 3,184
            CDSs (with protein)               :: 3,184
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3827
                     /organism="Aminobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_28257_length_3827_cov_2.730382"
                     /isolate="AMR_MDS_3026"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:3034609"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(136..1020)
                     /locus_tag="P5201_04675"
     CDS             complement(136..1020)
                     /locus_tag="P5201_04675"
                     /inference="COORDINATES: protein motif:HMM:NF014218.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytosine permease"
                     /protein_id="HRV97860.1"
                     /translation="MTHSRSNNLQHTRLLCEGGLFCVEAANGGAEAFVLKAETLGGLG
                     LGSAITVVVGSWIAGAVMGVDLFRFNRSAGAVLAGAAACFIFTNPLLNIVGYIGAVSV
                     GQFNYVEWMLEKNMLLALVGVLAWTTSLWTTNNAELYCNALYTGPVFWSYGRLIERKT
                     LVLWAGVLGTLLGSLAFYQIFFADFITVLGAAAPPLAGPLIADYYFVKKGRYIAENLE
                     NEPAVRMTGVLSFLAGGSLGLFFQYGAPLPYGLPSGLFALAATIVLYVLLYRRTQDAA
                     YDSALFAAADRKAQEVRS"
     gene            complement(1041..1949)
                     /locus_tag="P5201_04680"
     CDS             complement(1041..1949)
                     /locus_tag="P5201_04680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012869257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-adenosyl-l-methionine hydroxide
                     adenosyltransferase family protein"
                     /protein_id="HRV97861.1"
                     /translation="MKKRVLCTTVVLLFLLLFTSVASAKTALVFQTDFGLKDGAVSAM
                     KGVAFGVDAGLPMFDLTHEISPYSIWEAAYRLHQTMEFWPAGTVFVSVVDPGVGTERA
                     SVVALTKDGRYIVTPDNGTLTFIVETPGIEAVRTIDDARHRLSGSEQSYTFFGRDLYA
                     YTGAKLAAGAITFEEVGPLFEGEVVRIPYRKAEVKEGAVFGNIPVLDIQYGNVWSSVG
                     KALFDELKPRIGANFDVAIFRGEEKVFGGVIPYVNTFGDVPEGKPLLYFNSLMNLSLA
                     LNMDSFAETHGIESGPEWSLSVRPAE"
     gene            complement(1979..2650)
                     /locus_tag="P5201_04685"
     CDS             complement(1979..2650)
                     /locus_tag="P5201_04685"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRV97862.1"
                     /translation="MHFEILVEDQSGRKLLEILVPKIIGREHTFRVSAYKGIGRLPKG
                     LRSGEEARRRILLDQLPRLLRGYGRTFTYYPEDTVAVVVVCDLDDKCLKHFRRELLDL
                     LGRCEFKPKTRFCIAVEEGEAWLLGDLSAVKKAYPRAREPILKAYENDSICGTWERLA
                     DAVYPGGSRALSAAGWQVVGAEKSLWAEKIAPHMNVAENLSPSFCYFCRKIRELLEEC
                     SGALG"
     gene            complement(2655..>3827)
                     /locus_tag="P5201_04690"
     CDS             complement(2655..>3827)
                     /locus_tag="P5201_04690"
                     /inference="COORDINATES: protein motif:HMM:NF024700.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HRV97863.1"
                     /translation="KNGVGKSTLFDSFGFLSDCVKFGVEEACDIRGRGGFEKIRSQGC
                     DGPIEFEVYYKEDGNARPITYELSIGPDESGRPCVLMERLRQRRKGQKHGWPFSFLIL
                     NDGKGIVWKGEKEGRQIDESQKNLDLSGLLRAIQSDDAGEESKETEVVELEDRRRLGI
                     ATLGSLKQHPRISAFRRFIEGWYLSYFRPDSARALPLAGPQMHLNVHGDNIGNVVQYM
                     ERDQPARFRAVLDRIADRIPGIRKIDTERTSDGRLLLRFNDEGFQDPFYAQQMSDGTL
                     KLFAYMLLLADPTPPPFLCIEEPENGLYHKLLEILANEFRDHATGRKGSSQVFITTHQ
                     PYFVDALAPDEVWILEKGADGFSSIRRASDDALVRNMVEEGLPLGSLWYSDYLDAR"
BASE COUNT          851 a         1227 c          950 g          799 t
ORIGIN      
        1 agagatccgg accgagcggc agatgcggac cgtagctctc gcagctgccg aaaatcgtaa
       61 ttgcgctgtc gatctccccc gagccgatca tccgctccag ttcggaagag gaaaattcgt
      121 tcaaaaacac ctttctcatg accgcacctc ctgcgctttc ctgtccgccg ccgcaaagag
      181 cgccgagtcg tatgcggcgt cctgcgttct cctgtacagc agaacgtaaa gaacgatcgt
      241 ggctgcaagc gcgaagagcc cggacgggag accgtacggc aaaggcgcgc cgtactggaa
      301 gaagagcccc aggcttcccc cggcaaggaa ggagagcacg cccgtcattc tgaccgcggg
      361 ttcgttctcc aggttctccg cgatataccg cccctttttg acgaagtagt agtccgcgat
      421 gagcggcccg gccagcggag gggccgccgc ccccagcacg gtgatgaagt cggcgaagaa
      481 aatctggtag aacgccagcg aaccgagcag cgtccccagg actcccgccc acagcacaag
      541 cgtttttctt tcgatcaacc tgccgtacga ccagaagacc ggccccgtat acagcgcgtt
      601 gcagtacagc tccgcgttgt tcgtggtcca cagcgaggtc gtccacgcga gaacgccgac
      661 cagcgccagc agcatgttct tctccagcat ccattcgacg tagttgaact gccccaccga
      721 caccgcgccg atgtacccga cgatgttgag cagggggttc gtgaagatga agcaggccgc
      781 ggcgcctgca aggacagccc ccgcgctcct gttgaagcgg aagagatcca cgcccatcac
      841 agcccccgcg atccagctgc ccacgaccac cgtgatcgcg ctccccagtc ccaggcctcc
      901 gagagtctcc gccttcaaaa cgaacgcctc ggcgccgccg tttgccgctt cgacacaaaa
      961 gagccctcct tcgcacaata accgggtatg ttgcaagtta ttcgagcgag agtgcgtcac
     1021 aaacactact ccccctgcgg ctactccgcg gggcgaacgg aaagcgacca ctcaggcccc
     1081 gattctattc cgtgcgtttc tgcgaagctg tccatattca atgccagcga gaggttcatc
     1141 aggctgttga agtagagcaa gggcttcccc tccggcacgt cgccgaaggt gttgacgtac
     1201 ggaataacgc ctccgaagac cttttcctct ccccgaaaga tcgcgacgtc gaaatttgct
     1261 ccgatccggg gcttcagctc gtcgaagagc gccttgccga cgctgctcca gacgttgccg
     1321 tactggatgt cgagcacggg aatattgccg aagactgcgc cctccttcac ctcggccttc
     1381 ctgtacggaa tccggacgac ctctccctcg aagagcggcc cgacctcctc gaacgtgatc
     1441 gctcccgcgg ccagcttcgc ccctgtgtac gcatacaggt cgcgtccgaa gaaggtatac
     1501 gactgctccg acccagacag acggtgcctt gcgtcgtcga tcgtccgcac ggcctcgatg
     1561 ccgggcgtct ccacgatgaa ggtcagcgtg ccgttgtccg gcgtgactat gtatctgccg
     1621 tccttcgtca gggcgaccac ggaagcccgc tccgtaccta cgcccggatc gacaaccgac
     1681 acgaagaccg ttccggcggg ccagaactcc atcgtctgat gcagtctgta ggcggcctcc
     1741 cagatcgagt acggagaaat ctcgtgcgtc aggtcaaaca tcggcaagcc ggcatccacg
     1801 ccgaacgcca ctcccttcat cgcggaaacc gccccgtcct tcagtccgaa atccgtctga
     1861 aacacaagcg ccgtcttcgc agaggccacg ctcgtgaaga gcagcagaaa gagcaaaact
     1921 acagtagtgc acagaactct ttttttcatc ccacattcca ccttttcttc ttcacaattc
     1981 acccaagcgc cccggaacac tcttcaagca actcgcgaat ctttctgcaa aagtagcaaa
     2041 aacttggcga gagattctcg gctacattca tgtgcggagc gattttttcg gcccacaacg
     2101 acttctccgc tcctacgacc tgccatcctg ctgccgacaa agctcgcgag ccgcctggat
     2161 agacggcgtc ggcaagacgc tcccatgtac cgcaaattga atcgttctca tacgctttca
     2221 atatgggttc gcgcgccctc ggatacgctt ttttcactgc ggaaagatcg ccgagcagcc
     2281 acgcctctcc ctcttccacc gctatgcaga atcgagtctt cggtttgaac tcgcacctac
     2341 caagaagatc caaaagttct ctgcgaaaat gtttaaggca cttatcgtca agatcgcaca
     2401 ctacaacaac tgctaccgta tcctccgggt aataagtaaa tgttcttcca taccctctta
     2461 aaagccgtgg tagctggtcg agaagaattc gcctgcgtgc ttcctctcct gatcgaaggc
     2521 ctttgggaag acgtcctatt cccttgtagg cgctcactct aaaggtatgc tcccgtccta
     2581 taatcttcgg cacgaggatt tccaataact tcctccctga ttggtcctcg acaagtatct
     2641 cgaagtgcac ggcgctacct cgcgtccaga tagtcgctgt accaaagaga acccaaggga
     2701 agcccttcct cgaccatatt ccgtaccaag gcgtcgtcgc tcgccctcct gattgaagaa
     2761 aaaccgtccg cccccttctc aagaatccac acttcatcag gggcaagcgc atccacgaag
     2821 tacggctgat gggttgtgat aaaaacctgc gaacttccct tacgccctgt ggcatgatcc
     2881 cggaactcgt tcgcgagaat ctcgagcagt ttgtgataga gcccgttttc cggttcctct
     2941 atacagagga acggaggagg cgtagggtcc gcaagcaaaa gcatataggc gaaaagtttc
     3001 agcgtgccgt cggacatctg ctgcgcataa aaagggtctt gaaagccttc gtcgttaaac
     3061 ctgagcagta ggcgtccgtc gctcgttctc tcggtatcta ttttacgaat accggggatt
     3121 ctgtctgcaa ttctgtccaa aactgcccgg aaacgagccg gttgatcccg ctccatatac
     3181 tgcaccacgt ttcctatgtt gtccccatgt acattcaagt gcatctgcgg accggcgagc
     3241 ggaagagccc tggcggaatc gggccggaaa tagctcagat accacccttc gataaagcga
     3301 cggaaggctg aaatcctggg atgttgcttc agagaaccca aagttgctat accaagacgt
     3361 cttctgtctt ccagttctac aacctcggtc tccttcgact cctctcctgc atcatccgat
     3421 tgaatggcac gcagcaaccc cgagagatcc agattctttt gggactcgtc gatctggcgc
     3481 ccttcttttt cccctttcca gacaattcct ttcccgtcgt tcagtataag aaacgaaaaa
     3541 ggccaaccgt gcttctgtcc cttgcgcctc tggcgaagac gctccatgag gacacaggga
     3601 cgaccgctct cgtccggacc tatcgacaac tcgtaagtaa tagggcgcgc attaccgtct
     3661 tctttgtagt agacttcgaa ctcgatgggg ccgtcgcacc cctgagagcg tatcttctcg
     3721 aaacctcccc gtccgcggat atcgcacgcc tcttcaacgc cgaatttcac acaatcggag
     3781 agaaagccga atgagtcgaa cagagtgctt ttccccacac cgttctt
//
LOCUS       DAOTIL010000169         3824 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminobacteriaceae bacterium isolate AMR_MDS_3026
            NODE_28289_length_3824_cov_4.972672, whole genome shotgun sequence.
ACCESSION   DAOTIL010000169 DAOTIL010000000
VERSION     DAOTIL010000169.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868050
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminobacteriaceae bacterium (wastewater metagenome)
  ORGANISM  Aminobacteriaceae bacterium
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae.
REFERENCE   1  (bases 1 to 3824)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3824)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:19:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,224
            CDSs (total)                      :: 3,198
            Genes (coding)                    :: 3,184
            CDSs (with protein)               :: 3,184
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3824
                     /organism="Aminobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_28289_length_3824_cov_4.972672"
                     /isolate="AMR_MDS_3026"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:3034609"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..311)
                     /locus_tag="P5201_04695"
     CDS             complement(<1..311)
                     /locus_tag="P5201_04695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009202117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OadG family protein"
                     /protein_id="HRV97864.1"
                     /translation="MSIAPAATAVTETVDAAAAAAASASARIAEEMSGVGGALTMSII
                     AFSVVFLVLGGLSGVIYAIKYMAQALERKKNEPPAGNATAGNAAAPAPVAAASAAQRG
                     A"
     gene            complement(337..1896)
                     /locus_tag="P5201_04700"
     CDS             complement(337..1896)
                     /locus_tag="P5201_04700"
                     /EC_number="6.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006299990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016874 - ligase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA carboxylase subunit beta"
                     /protein_id="HRV97865.1"
                     /translation="MSQRTIEELCEELLLKREKAQHGGGEKAVAKQREKGKGTARERI
                     EKLLDPGTFIEVDEFVRHRCVNFGLEKTVFDGDGVIIGWGLVEGRKVYVYSQDFTVLG
                     GSLGEMHAAKICKIMDMAMSTGCPVVGINDSGGARIQEAVDSLNGYGAIFSRNTIASG
                     VIPQLSVIAGPTAGGAVYSPALTDYIFMVEGTGIMHITGPAVIKAVTGEEVTSEQIGG
                     SMANNSKSGNAHFSAKSEDECIAQVRKLLSYLPQNNLEEAPEKETGDDPLREDAELRN
                     IVPVNPNKGYDVREVIRRVVDESDFFEVQPLWAKNILTGYARLGGKVIGIIANQANHL
                     AGCLDIDASDKASRFIRHCDAFNIPLVTFVDVPGYLPGTAQEFGGIIRHGAKMLYAYS
                     EATVPLITVVLRKAYGGAYLGMCSKALGADIVLAWPQAEIAVMGAEGAANIVFRREIE
                     AAEDSVATRTRKIEEYREAFANPYVAAERGLVDRVILPEATRGEIWRALVGAESKRDL
                     RPRRKHGVMPH"
     gene            complement(1924..2874)
                     /gene="meaB"
                     /locus_tag="P5201_04705"
     CDS             complement(1924..2874)
                     /gene="meaB"
                     /locus_tag="P5201_04705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012869690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016772 - transferase activity,
                     transferring phosphorus-containing groups [Evidence IEA];
                     GO_process: GO:0015819 - lysine transport [Evidence IEA];
                     GO_process: GO:0015822 - ornithine transport [Evidence
                     IEA];
                     GO_process: GO:1903826 - L-arginine transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl Co-A mutase-associated GTPase
                     MeaB"
                     /protein_id="HRV97866.1"
                     /translation="MDTLLEKALGGDIRSIARIISLIENDNPEKDTLLKRVYPMTGKA
                     IVLGITGSPGAGKSTLTDKVIEHFRRQGKKVGVIAVDPSSPFSGGAILADRIRMQRHT
                     GDSGVYIRSMGTRGSLGGLSRGTRESVILLDACGYDVIIIETVGVGQSEVDIIKIADT
                     VCLVLVPGMGDDIQIMKAGIMEIANVFAINKADKPGVDRVAAEVRLMLDLVKDRPWVP
                     PVHLTVAENGTGIEELVASIASHSVFLKSNPDGVRREWRRLAFEVEEILLQHITRMTE
                     KSWNAYKTDEVMELLLSRKSNPYAVAEDVLRKIIPGTRVV"
     gene            complement(2885..3298)
                     /locus_tag="P5201_04710"
     CDS             complement(2885..3298)
                     /locus_tag="P5201_04710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005662094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cobalamin B12-binding domain-containing protein"
                     /protein_id="HRV97867.1"
                     /translation="MDERKIRVVVAKPGLDGHDRGAKVVARALRDAGMEVIYTGLRQT
                     AEQIVETVLQEDADAVGISILSGAHTFYFTKIIDMLREKDAGDVIVFGGGVIPDSDIP
                     GLIEAGAGAVFGPGTPTTVCVEWLEGAVAKKRAKE"
     gene            complement(3349..>3824)
                     /locus_tag="P5201_04715"
     CDS             complement(3349..>3824)
                     /locus_tag="P5201_04715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013048283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016866 - intramolecular transferase
                     activity [Evidence IEA];
                     GO_function: GO:0031419 - cobalamin binding [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="methylmalonyl-CoA mutase family protein"
                     /protein_id="HRV97868.1"
                     /translation="AGSYVVESLTNEVEKKAFEYIQQIDEMGGMLTAIEKGYVQKHIQ
                     DAAYEFQRAVESKENVVVGVNKFQIEEKNKDMKLLTVDASVGERQIKKLQELKASRDN
                     VAVSNALDAIRSAALNESQNLMPLIIDAVRVYATEGEICGVLRGVFGEYTENIVL"
BASE COUNT          670 a         1161 c         1026 g          967 t
ORIGIN      
        1 tcggctcctc tctgcgccgc ggaggcggcg gcgacgggcg ccggcgctgc ggcgtttcct
       61 gctgtcgcgt ttcctgcggg cggttcgttc ttcttccgtt ccagcgcctg cgccatgtac
      121 ttgatggcgt agatgactcc gctcagccct ccaagtacca aaaagacgac gctgaaggcg
      181 attatggaca tggtgagcgc tcctccgact ccgctcatct cctccgctat gcgcgcggag
      241 gctgacgcgg ctgcggcggc ggctgcgtcg actgtctcgg ttacggcggt cgctgcgggt
      301 gcgatgctca ttcgtgcgtc actccttcct tggtatttag tgcggcatga cgccgtgctt
      361 gcgccgcggg cgcaggtcgc gcttgctctc cgctcctacg agcgctctcc agatctcgcc
      421 gcgcgtggct tcgggcagga tgacacggtc gacgaggcct cgttcggcgg cgacgtaggg
      481 gttggcgaag gcttcacggt attcttctat tttcctggtg cgcgtggcga cgctgtcttc
      541 cgctgcttct atctcgcgtc ggaagacgat gttcgcggcg ccttcggctc ccatgacggc
      601 gatttcggcc tgcggccatg ccaatacgat gtcggctccc agggctttgc tgcacatgcc
      661 gaggtacgcg cctccgtacg ctttcctcaa tacgacggtg atgaggggga cggtggcttc
      721 gctgtacgcg tagagcattt tggcgccgtg gcggatgatg cctccgaatt cctgcgctgt
      781 gccggggagg tatccgggga cgtcgacgaa ggtgacgagc gggatgttga aggcgtcgca
      841 gtggcggatg aaccggctgg ctttgtccga ggcgtcgatg tcgaggcatc cggcgaggtg
      901 gttggcctgg ttggcgatga tgccgatgac tttgcctccg agacgcgcgt atccggtgag
      961 gatgttcttg gcccacagcg gctggacttc gaagaagtcg ctttcgtcga cgactctccg
     1021 gatgacttcc ctcacgtcgt accctttgtt ggggttgacg gggacgatgt tcctcagttc
     1081 ggcgtcttct ctgagggggt cgtctcccgt ctctttctcg ggcgcttctt ccaaattgtt
     1141 ctgcgggagg tagctcagga gcttcctgac ctgcgcgatg cattcgtctt cgctcttggc
     1201 gctgaagtgc gcgttcccgc tcttgctgtt gttggccatg gagccgccta tctgttcgct
     1261 ggtgacttct tcgccggtga cggctttgat gacggcgggg ccggtgatgt gcatgattcc
     1321 cgttccttcg accatgaaga tgtagtcggt gagcgcgggg ctgtacacgg cgcctccggc
     1381 ggtggggccg gctatgacgg agagctgcgg gatgacgccg ctcgctatgg tgttccggga
     1441 gaagatcgct ccgtacccgt tgagcgagtc gacggcttcc tggatcctcg ctccgccgct
     1501 gtcgttgatg ccgaccacgg gacatccggt gctcatggcc atgtccatta tcttgcagat
     1561 cttcgcggcg tgcatctccc cgagcgaccc gccaagtact gtgaagtcct ggctgtagac
     1621 gtacactttg cgcccttcca ccagtcccca cccgatgatg actccgtctc cgtcgaagac
     1681 cgtcttctcc agtccgaagt tcacgcaccg gtgccggacg aattcgtcca cttcgatgaa
     1741 cgtaccgggg tcgagcagct tctctatacg ctcccgcgcc gttcccttcc ccttctcacg
     1801 ctgcttggcg accgctttct caccgcctcc atgctgcgct ttctcacgct tcagcaacag
     1861 ctcctcgcag agctcttcta tcgttcgctg cgacatctga tgaatctgcc cccttcgttc
     1921 ccctcagacc actctggtcc cgggaataat ttttctcaat acatcctctg caacagcata
     1981 agggttgctt tttctcgaaa gcaacaactc cataacttca tctgttttgt acgcgttcca
     2041 agatttttcc gtcattcttg taatgtgctg cagtaaaatc tcttcgactt caaaagcgag
     2101 acgccgccat tcacgccgca ctccgtcagg attgcttttg aggaaaacac tgtgtgacgc
     2161 gatacttgca accagctctt ctattccggt cccgttctcc gcaacagtca ggtgcaccgg
     2221 cggaacccac gggcgatcct ttacgagatc aagcatcagt cgaacttcgg cggcgactct
     2281 gtccactccc ggtttgtccg ccttgtttat ggcaaagacg ttggctatct ccatgatacc
     2341 cgctttcatg atctggatat cgtcgcccat gccgggaact agaaccagac agaccgtatc
     2401 ggcaattttg ataatatcca cttccgattg ccctacgccg acggtttcga tgataatgac
     2461 atcatagccg caggcatcaa gtagaattac ggactctctt gttcctctgc tgagtccgcc
     2521 gagggaacct cgggtcccca tgctccgaat atatactccg gagtcgcctg tatgccgttg
     2581 catccgaatt ctgtccgcaa ggatagcgcc tccggaaaag ggactcgacg gatcaactgc
     2641 gatgaccccg acttttttcc cctggcggcg gaagtgctct attaccttgt ccgtgagcgt
     2701 acttttgccg gcccccgggc ttccagtaat cccgagaacg atcgctttgc cggtcatcgg
     2761 gtatacacgc ttaaggaggg tatccttctc cgggttgtcg ttttcgatca ggctgataat
     2821 cctggcgata gagcggatgt ctcctccaag cgccttttcg agcaaagtat ccacagccaa
     2881 tggactactc tttcgcccgt ttttttgcta ccgccccttc aagccactcg acacaaacag
     2941 ttgtcggcgt tccaggacca aaaacggcgc ccgcgccggc ctctataaga ccggggatat
     3001 cgctgtctgg aatgactcct ccgccgaaca cgataacatc tccggcgtcc ttctccctga
     3061 gcatgtcgat aatcttggtg aaataaaacg tatgagctcc ggagaggatg ctaattccca
     3121 ctgcgtcggc gtcttcctgc agcacagtct ccacgatctg ctccgcggtt tggcgaagac
     3181 cggtataaat gacctccatt cctgcgtctc taagcgcgcg cgctacgacc ttcgcgcctc
     3241 tgtcatggcc gtcaagaccc ggtttcgcca ccacaacccg tatttttctc tcatccatgg
     3301 aagatccctc cattatgata atcctgatgc acagccttaa ttaaaaggct acaaaacgat
     3361 attttctgta tattcgccga agacgccgcg gagtacgccg cagatttcac cttcagtcgc
     3421 atagacacgg actgcgtcaa taataagcgg cataagattc tgcgactcgt tcaaagcggc
     3481 tgagcggatg gcgtcaagag cgttggacac ggcaacattg tcacgggagg ctttcaattc
     3541 ttgcagcttc ttgatctgcc gctctccgac ggaggcgtcg acagtcaaaa gcttcatatc
     3601 tttgttcttc tcctctatct ggaacttgtt gactccgacg acaacatttt cttttgattc
     3661 cacagcacgc tgaaattcgt acgctgcatc ctggatgtgc ttttggacat accccttctc
     3721 aatcgccgtc agcatgccgc ccatttcgtc gatctgctga atatactcga acgccttctt
     3781 ttcaacttca tttgtgaggg attctactac gtagcttcct gcaa
//
LOCUS       DAOTIL010000170         3806 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminobacteriaceae bacterium isolate AMR_MDS_3026
            NODE_28457_length_3806_cov_2.553719, whole genome shotgun sequence.
ACCESSION   DAOTIL010000170 DAOTIL010000000
VERSION     DAOTIL010000170.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868050
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminobacteriaceae bacterium (wastewater metagenome)
  ORGANISM  Aminobacteriaceae bacterium
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae.
REFERENCE   1  (bases 1 to 3806)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3806)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:19:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,224
            CDSs (total)                      :: 3,198
            Genes (coding)                    :: 3,184
            CDSs (with protein)               :: 3,184
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3806
                     /organism="Aminobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_28457_length_3806_cov_2.553719"
                     /isolate="AMR_MDS_3026"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:3034609"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..894)
                     /locus_tag="P5201_04720"
     CDS             complement(<1..894)
                     /locus_tag="P5201_04720"
                     /inference="COORDINATES: protein motif:HMM:NF013200.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit
                     alpha/FixB family protein"
                     /protein_id="HRV97869.1"
                     /translation="MNKQDYNGVMIVVQQMDNRISGVSYELIGKGKELAEALRTEVTA
                     VLLGYEVESLCAALGKYGADRVLMVDDPALETYMTEPYARAMAAIIERFKPEIVLFGA
                     TAIGRDLAPRLSARVHTGLTADCTKLEIDPETRDLMMTRPAFGGNIMATILCSNHRPQ
                     MATVRPGVMQRIAPLESVECPVERVRVDGLAEGKNVEILEIVKKENKRQDIQEAKVLV
                     SGGRGMGGPENFSMLEKLADALGGTVSSSRASVDAGWAEKDRQVGQTGRTVRPNLYIA
                     CGISGAIQHLAGMEESDVIVAI"
     gene            complement(913..1695)
                     /locus_tag="P5201_04725"
     CDS             complement(913..1695)
                     /locus_tag="P5201_04725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016293394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit beta/FixA
                     family protein"
                     /protein_id="HRV97870.1"
                     /translation="MKIIVCVKQVPDTNEVRLDPVTGTLMREGVPSIVNPDDKAGLEA
                     ALRLKDQYGAHVTVLSMGPPQADLALRETLAMGADEAVLLTDKAFSGADTWATSSTLA
                     SALSDMEYDLIITGRQAIDGDTAQVGPQIAEHLGLVNISYAEDLRVEDDCVVVKRQFE
                     DRYHIIKAKMPCLITALSEMCEPRYMTPGGIFDAYRSREIKTLVRSDLKIDDENIGLK
                     GSPTRVVKSFTKAVKGKGTVVALDAHDAVDWITDRLQEKFIL"
     gene            complement(1708..2847)
                     /locus_tag="P5201_04730"
     CDS             complement(1708..2847)
                     /locus_tag="P5201_04730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016293395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase"
                     /protein_id="HRV97871.1"
                     /translation="MSFALSKEQRMAQQLFRRFAEKEVKPLAQEVDENERFPSETVEK
                     MARFGFLGIPYGKEWEGQGCDTLTYILCVEEMSRFCATTGVIVSAHTSLGCDPIAKYG
                     TPEQKEKYLPDLLSGRKLGAFALTEPGAGTDAAGQQTKAVLDGENYVLNGSKIFITNA
                     GQADVYIIFAMTDKSLGTKGISAFIVEKEFPGFSVGKKEKKMGIRGSSTCELIFENCL
                     VPKENMLGKEGKGFGIAMGTLDGGRIGIGAQALGVAQGALDETVAYVKERKQFGKPIS
                     AFQNTQFQLAEMQTRIEAARHLVYDAAATKDSGARFSVEAAMAKLYASQTAMEVTTKC
                     VQLHGGYGYMRDYPVERMMRDAKITEIYEGTSEVQLMVVAGALLK"
     gene            complement(2943..3359)
                     /locus_tag="P5201_04735"
     CDS             complement(2943..3359)
                     /locus_tag="P5201_04735"
                     /inference="COORDINATES: protein motif:HMM:NF024844.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MaoC family dehydratase"
                     /protein_id="HRV97872.1"
                     /translation="MKGKTIHDLNIGDEAVFQKTLTETDVYLFAGVSGDQNPAHINEV
                     YAAGTRFKTRIVHGMLTASLVSAALGMALPGPGTIYLEQTLKFPAPTRIGDTVEAKVK
                     VVEMLPEKNIVKLETTCMNQAGELLLKGTATVMPPK"
     gene            complement(3417..>3806)
                     /locus_tag="P5201_04740"
     CDS             complement(3417..>3806)
                     /locus_tag="P5201_04740"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacid CoA-transferase"
                     /protein_id="HRV97873.1"
                     /translation="GFINITQTAKKVIYCGTFTAGGLEIHAENGALKIIKEGKIKKFI
                     DDVEQITFSGTYAVEKGQPVLYVTERAVFELTEDGLTLTEIAPGVSLENDVLAHMDFK
                     PRIAGDLKAMDSRIFRREPMGIHAAAR"
BASE COUNT          804 a         1153 c          905 g          944 t
ORIGIN      
        1 gatcgccacg atcacatcgg actcctccat accggccaga tgctgaatcg ccccggaaat
       61 gccgcatgcg atataaagat tgggacgtac cgtccttccc gtttgcccca cctgacgatc
      121 cttctccgcc catccggcgt cgacgcttgc acgggaggag gaaactgtcc cgccgagcgc
      181 gtccgccagt ttttcgagca tggaaaagtt ttcaggacct cccattccgc gacctccgga
      241 aacgagaact ttcgcctcct ggatgtcctg tcttttattc tcctttttca cgatctccaa
      301 aatttcaaca ttctttccct cagccagccc gtctacgcga acgcgttcca cagggcattc
      361 tacactctcc aacggagcaa tgcgctgcat gacgcccgga cggacggtcg ccatctgagg
      421 ccggtggttc gagcaaagaa tcgtcgccat gatattaccg ccgaaggccg ggcgggtcat
      481 cataagatcg cgcgtctccg ggtcgatttc cagtttggta caatccgcgg tcagaccggt
      541 atgaactcgc gccgataagc gaggtgcgag atctcggccg atagccgtcg cgccgaacag
      601 aacgatttcg ggtttgaaac gttctatgat cgcggccatc gcgcgcgcgt acggctccgt
      661 catatacgtc tccagcgcgg gatcgtcgac cataagaacc ctgtccgctc catattttcc
      721 caatgcggcg cacagcgact ccacttcata ccccagcaga acggcagtaa cctccgtccg
      781 caatgcttcg gccaactcct tgccctttcc tatcagttcg taggagaccc cggagatccg
      841 attatccatc tgctgcacca caatcataac gccgttgtaa tcctgtttgt tcatcgggct
      901 ctcacctctc catcacagaa tgaacttttc ctgcagacga tccgtgatcc agtcgacggc
      961 gtcgtgagcg tcgagcgcaa cgactgtacc ttttcccttt accgcctttg taaaagattt
     1021 gacgactcgc gtaggagagc ctttcaaacc gatattttca tcgtctattt tcaggtcgga
     1081 tcggacgaga gttttgatct cccgcgagcg ataggcatcg aagatgcctc cgggcgtcat
     1141 gtaacgaggt tcgcacatct ccgaaagcgc agtgataagg cagggcatct tcgctttaat
     1201 aatatgatag cggtcttcga attgtctctt gacgacgacg caatcgtctt caactcgcaa
     1261 atcctccgca tagcttatat tcacaagccc caggtgttcc gctatttgcg gtcccacctg
     1321 cgccgtgtct ccatcgatgg cctgtctgcc ggtaatgatc aaatcgtact ccatatccga
     1381 aagggcggaa gcgagagtac ttgaagtcgc ccatgtatcg gccccgctga aagccttgtc
     1441 cgtcagaagg acggcttcgt cggctcccat agccaatgtt tcgcgcagag cgagatcggc
     1501 ctgagggggt cccatcgaga gaacggtcac atgcgctccg tactggtctt taagccgcaa
     1561 cgccgcctcc agaccggcct tgtcgtcggg attgacgata ctgggaacgc cttcgcgcat
     1621 caatgttccg gtcacgggat ccagccgcac ttcattggta tccggcacct gtttcacaca
     1681 aacaatgatc ttcatgttct caccccttta tttcagcaag gcgccggcga caaccatcag
     1741 ctgcacttcg ctggtacctt cgtagatctc cgtgatcttc gcgtcgcgca tcattctctc
     1801 cacggggtag tcgcgcatat atccgtatcc gccgtgaagc tgcacgcatt tcgtggtaac
     1861 ttccatagcc gtctgagagg cataaagctt ggccatcgcc gcctcgacgg aaaatctggc
     1921 tccggaatcc tttgtcgctg cggcgtcata gaccagatgt ctcgcagcct cgatccgcgt
     1981 ttgcatttcg gcaagctgga actgcgtgtt ttgaaaagcg gagatcggct tcccgaactg
     2041 cttccgctct ttgacgtagg ccacggtttc atccaacgcc ccctgcgcga ctccgagcgc
     2101 ctgagctccg attccgatgc gtcctccatc caaagtcccc atcgctatac cgaacccttt
     2161 cccttccttg ccgagcatat tctccttagg aaccaggcaa ttctcaaaga taagctcgca
     2221 cgttgaagaa ccccgtatcc ccatcttctt ctctttcttg cctacggaaa accccgggaa
     2281 ttccttctcg acgatgaaag ccgaaatacc ttttgttccg aggctcttgt cggtcatcgc
     2341 aaagataata tacacatcgg cctgtccggc gttggtaata aatatcttgc tgccgttcag
     2401 aacataattt tctccgtcaa ggacagcctt cgtctgttgc ccggccgcat cggtccccgc
     2461 tccgggctcc gtaagcgcaa aagcgccgag ctttcttccg gagagaagat cgggaagata
     2521 tttttctttt tgctcagggg taccatactt ggcgatcgga tcgcacccca acgacgtgtg
     2581 cgcagaaacg atgactcctg tcgtcgcgca aaatcggctc atttcttcaa cgcagagaat
     2641 ataggtcaga gtgtcgcacc cctgcccttc ccattctttc ccgtacggga tgccgaggaa
     2701 gccgaatcgg gccatctttt ccaccgtctc ggaaggaaac cgttcgtttt catccacttc
     2761 ctgcgccaag ggctttactt ccttttccgc gaaacgacga aaaagttgct gcgccattcg
     2821 ctgttcttta ctcagtgcaa agctcatcta ttcgccctcc aatcatcttc gcaaacactt
     2881 gagtttgtaa aaaaacacaa tggagaggcg tcgtggaaca cggacaacgc ctctccggga
     2941 agctacttcg gaggcatcac cgtcgccgtc cccttcaata aaagctcccc agcctgattc
     3001 atgcatgttg tctccagttt gacgatattt ttttccggaa gcatctcgac cactttcact
     3061 tttgcctcga cagtgtctcc tatgcgcgtc ggggcgggga atttaagagt ctgctccagg
     3121 tagatagtgc cgggaccggg aagcgccatg ccgagcgcgg cggatacaag agaagccgtc
     3181 aacatcccgt gcacaattcg agttttgaaa cgcgtaccgg ccgcgtacac ttcgttgata
     3241 tgcgcagggt tttgatctcc gctgactccg gcgaacagat agacgtcggt ctccgtcagc
     3301 gttttttgaa acacggcttc gtctcctatg ttcaaatcat ggatcgtttt tcccttcatt
     3361 tctttccctc ctcaatgttt tcagtaaagg aaaccggtac agaaatattt ctcgctctat
     3421 cgagcagcgg catgtatccc cataggctca cgacggaaaa tacgggaatc catggccttg
     3481 agatctcccg cgatgcgcgg tttgaaatcc atatgcgcca aaacgtcgtt ttccaaagag
     3541 acgcccgggg cgatctcggt cagcgtcagc ccgtcctcgg tgagttcgaa gacggcgcgc
     3601 tccgttacat aaaggacggg ctgccccttt tccacagcgt atgttccgct gaaggtaatc
     3661 tgctccacat catcgatgaa ttttttgatt ttcccctctt taatgatctt cagcgcaccg
     3721 ttttcggcat gtatttccaa tcctcccgcg gtgaaggtac cgcaatagat cacttttttc
     3781 gcggtctggg tgatgttgat gaaccc
//
LOCUS       DAOTIL010000171         3798 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminobacteriaceae bacterium isolate AMR_MDS_3026
            NODE_28533_length_3798_cov_3.013625, whole genome shotgun sequence.
ACCESSION   DAOTIL010000171 DAOTIL010000000
VERSION     DAOTIL010000171.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868050
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminobacteriaceae bacterium (wastewater metagenome)
  ORGANISM  Aminobacteriaceae bacterium
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae.
REFERENCE   1  (bases 1 to 3798)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3798)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:19:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,224
            CDSs (total)                      :: 3,198
            Genes (coding)                    :: 3,184
            CDSs (with protein)               :: 3,184
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3798
                     /organism="Aminobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_28533_length_3798_cov_3.013625"
                     /isolate="AMR_MDS_3026"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:3034609"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..229
                     /locus_tag="P5201_04745"
     CDS             <1..229
                     /locus_tag="P5201_04745"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRV97874.1"
                     /translation="KFKQKYGGHHRFVLYKNLRKPGAIKTRGHKEILLRNYDYHPKVF
                     ADGFVGYKIKHKSNGMAMLYGKPLEGAPSKA"
     gene            229..1437
                     /locus_tag="P5201_04750"
     CDS             229..1437
                     /locus_tag="P5201_04750"
                     /inference="COORDINATES: protein motif:HMM:NF013871.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRV97875.1"
                     /translation="MNSVPAQGMETLRLLDRAGFESALVGGAVRDLLLGRTPGDIDIA
                     TEASFSALAALFPGGKSVGPEGKPVFLVPCGERRCEITSFCGGSLETDLARRDFTVNA
                     LALRSTGELVGTRRSKLDIELRLLRFNGSAADRLREDPLRALRLPRFAATLPGFSVAG
                     GAYSAVRAAAPSFARCAPERIGREVRLGLEGDPGLFLRGLAASRTLHILRSLFGGGPG
                     EPALLLQSLKRLDDRKEPLLRRTALLASFRRKLSFPDLEGASSVRAILEGWRWPKQVC
                     SGCAALVCRRAAPAANMSRAEMADLLLAHGEAFWESVFALFGALCRVHVPRRRMALFR
                     ERFGRLAVRLRTEEALIPSGDELMERYALSSGPEVGRLLKALRTEHLLKGFASREEAM
                     ERVASLMNIR"
     gene            1534..2793
                     /locus_tag="P5201_04755"
     CDS             1534..2793
                     /locus_tag="P5201_04755"
                     /inference="COORDINATES: protein motif:HMM:NF020252.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HDOD domain-containing protein"
                     /protein_id="HRV97876.1"
                     /translation="MGLLHVDQLQAGMVLEKNLLAFNGRFLLPRGIVLTEKHIQTMKV
                     WGVVEASVEGDLPAEPLKDAAASMPPEVVQEAGRSLETLFPSTPSTSPMKDFYSVAME
                     RTTAALMRDRSQRGAQAQQPRSPARRLSSNIPNLSVTASDLVSRELQLASLPDVYYRI
                     MEVINSTRSSASHIAEAVGKDTSLLSRLLRLVNSAFYCFPSKIETVTRAVAIVGTREL
                     STLALGVAAVQYFNDIPSEYVDMKSFWRHSVACGVYARLMASEKIGVSEERLFVSGLL
                     HDLGKLVLFRQVPLSARNAIELALAKKIPLCEAEREILGFDHAYLGALLLREWKIPSP
                     LENAVRFHHSPLSSSSPLDPALLASADALAMATGFSGSGSVVMPQISPEIWAETGLSA
                     AVFQPVILQGGRQIEDITNAFLGNGGA"
     gene            2795..>3798
                     /locus_tag="P5201_04760"
     CDS             2795..>3798
                     /locus_tag="P5201_04760"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRV97877.1"
                     /translation="MLRRNRISFMEERLRRLEEQNAYLWQEKAFGIEALDAAASLGHF
                     DTSMNKLEDPLPILRETVKRVQGLIPLKAAVIYLVGDDSSFYPAYCSPPDALPELEKR
                     VATLIEDRTFVWAIKRRKPVLTEFKEKRAPLLLHVLSTISRTRGMLVGVMDKGWEEIS
                     DYSLSLLSVALTASAGALESFELYRNLRSLNRDLSSKMAQLEDSRNELERYHVVLEEQ
                     VETRTRELAQANDEMKMEIAERMRAEEELRSSESMLKHALEGVGEGIWMWDVASGEVD
                     LSPLSMEILGCAADVAACPPEMRWMEGWERLIHPDDVEKVRYKKQKCLAGDTQGYRVE
                     "
BASE COUNT          694 a         1121 c         1192 g          791 t
ORIGIN      
        1 caagttcaag cagaagtacg gcgggcatca ccgtttcgta ctctacaaga acctgaggaa
       61 accgggtgcg ataaagacgc gggggcacaa ggagatactc ctcagaaact acgactacca
      121 tccgaaggtc tttgccgacg gcttcgtagg gtacaagatc aagcacaagt cgaacggaat
      181 ggcgatgctc tacggcaagc ctctggaggg agcgccgtcg aaggcgtgat gaacagcgtg
      241 ccggcgcagg gaatggagac gctccgtctg cttgatcgcg ccggtttcga aagcgcgctc
      301 gtggggggcg ccgtgcggga tcttcttctc ggacggactc ccggggatat cgacatcgcg
      361 acggaggcct ccttttccgc tctcgccgcg cttttccccg gcgggaagag cgtcggacca
      421 gagggaaaac cggtctttct tgttccctgc ggagagaggc gctgtgaaat cacctccttc
      481 tgcgggggct cacttgaaac ggacctggcg cgccgtgact tcacggtgaa cgcgctcgcc
      541 ctccgctcca caggcgagct tgtcggaacc cgccgcagca aactggatat cgagcttcgg
      601 ctgctgcgct tcaacgggag cgcagccgac cggctcaggg aagatccgct cagggcgctt
      661 cgtttgccgc gtttcgcggc gacgcttccc ggtttttccg tggccggggg cgcctattcc
      721 gccgtgaggg ccgcggcgcc gtcgttcgcc cgttgcgccc ccgaacggat aggaagagaa
      781 gtgcgtctcg ggctcgaagg cgatccgggg cttttcctgc gcggtctggc ggcaagccgc
      841 acgcttcata ttctccgttc cctgttcggc ggcgggccgg gggagccggc gcttcttctt
      901 caatcgctga agaggcttga cgaccggaaa gaaccccttc tcagacggac ggcgcttctc
      961 gcctcgttcc ggaggaagct ctccttcccc gacctcgaag gcgcctcctc ggtccgcgca
     1021 atactggaag gatggcgctg gccgaaacag gtgtgctcgg gatgcgcggc gcttgtgtgc
     1081 aggagggccg cccctgcggc gaacatgtcg cgggcggaga tggccgatct gcttcttgct
     1141 cacggcgagg ccttctggga gtcggttttt gctcttttcg gcgctctctg ccgagtgcat
     1201 gtcccgcgca ggcgcatggc tctcttccgg gagcgtttcg gccgcctcgc cgttcgcctc
     1261 cggacggaag aagcgctcat tccctccgga gatgagctga tggagcggta tgcgctctct
     1321 tcaggtccgg aggtggggag actcttgaag gctcttcgga ccgaacatct tctcaaggga
     1381 ttcgcttcga gggaggaggc gatggagaga gtcgcttcgt tgatgaatat acgatgaaga
     1441 gaggaactcc cctctctgtt ttccgctctc ttcgtgttac aatcagaaga aacatatttg
     1501 tatctgcaga gggtatagag ggagtcgatt ttcatgggtc tgcttcatgt cgatcagttg
     1561 caagcgggca tggtactgga gaagaatctg ctggctttca acggccgctt tcttctccca
     1621 aggggtattg tgcttacgga gaagcatatt cagacgatga aggtctgggg ggttgtagag
     1681 gcgtccgttg agggagatct ccctgcggag cctctgaagg atgccgcagc ctcaatgcct
     1741 cccgaggttg tgcaggaggc cgggcgctcg ctggagacgc tcttcccctc cactccgtcg
     1801 acttcgccga tgaaagattt ctactctgtc gccatggaac ggacaaccgc agccctgatg
     1861 cgcgaccggt cgcagcgcgg ggcgcaggcg caacagcccc gcagccccgc gcgccggttg
     1921 tcctcgaata tccccaatct gtctgtgaca gcctccgatc tcgtctccag ggagctgcag
     1981 cttgcctctc tccccgatgt gtactatcgg atcatggagg tgatcaattc gacccgcagc
     2041 tccgcctccc atatagccga ggccgtcggg aaggatacca gtcttctttc ccggcttctt
     2101 cgtcttgtca acagcgcgtt ctactgcttc ccctccaaga tagagaccgt gacgcgggcc
     2161 gttgcgatcg tcggaacccg cgagctttcg acgttggcgc tcggggttgc ggccgtccag
     2221 tacttcaacg acatcccctc ggaatatgtg gacatgaaga gcttctggag gcactccgtg
     2281 gcctgcggcg tctacgcccg tctgatggcc tcggagaaga tcggcgtttc cgaggaacgt
     2341 ctcttcgtct ccggactgct gcacgacctg ggaaagctcg tgctcttccg tcaggtgccc
     2401 ctttcggcgc ggaacgccat cgaattggct cttgccaaga aaattcccct ctgcgaagcg
     2461 gagcgggaga tcctcggctt cgaccacgcc tatctcgggg cgttgctgct gcgggagtgg
     2521 aaaattccct cgcctctcga aaacgccgta cggtttcatc actccccgct ttcctcttcc
     2581 tctcctcttg atcctgcgct tctggcgtcg gcggatgcgc tcgccatggc gacaggattc
     2641 agcgggagcg gctcggtcgt gatgccccag atctcgccgg agatatgggc ggagacgggg
     2701 ctttcagcgg ccgtcttcca gccggtgatc cttcagggag gccgccagat agaggacatt
     2761 acgaacgctt ttctcggaaa cggaggcgca tagcatgctc cgtcgcaatc gcatctcttt
     2821 tatggaggaa cgcctccgcc gcctggaaga acagaacgca tacctctggc aggaaaaagc
     2881 attcggcatc gaggcgcttg atgccgctgc ttcgttgggg cacttcgaca ccagcatgaa
     2941 caagctcgaa gacccgctgc cgattctccg ggagactgtc aagcgagtgc aggggcttat
     3001 ccctctgaaa gcggccgtga tatatctcgt cggcgacgac tcttcattct accccgcgta
     3061 ctgctccccg cccgatgcgc ttcccgagct ggagaagcgt gttgctactc tcatcgagga
     3121 cagaacgttc gtctgggcga tcaagaggcg caaacctgtc ctcaccgaat tcaaggaaaa
     3181 gcgcgctccg ctgctgctcc acgtgctctc caccatctcc aggacgcggg gcatgctggt
     3241 aggggtcatg gacaaggggt gggaggagat ctcggactac tccctctctc ttctctccgt
     3301 cgctctgact gcaagcgcgg gagctctgga gagctttgag ctgtaccgga atcttcgcag
     3361 tctcaacaga gatctctcct cgaagatggc gcagctggag gactcgcgga acgagcttga
     3421 acggtatcac gtggttctgg aggaacaggt ggagactcgt accagagagc tggcgcaggc
     3481 gaacgacgag atgaagatgg agatcgccga acggatgcgc gccgaggaag agcttcgttc
     3541 cagcgagagt atgctcaaac acgcccttga gggtgtcggc gaaggaatat ggatgtggga
     3601 cgtcgcttcg ggagaggtcg atctttcgcc gctttcgatg gagattctcg gatgcgccgc
     3661 cgacgtcgcc gcctgcccgc cggagatgcg gtggatggag gggtgggaac ggctcattca
     3721 cccggatgat gtggagaagg tccggtacaa gaagcagaaa tgccttgccg gtgatacgca
     3781 ggggtacaga gtcgagta
//
LOCUS       DAOTIL010000172         3782 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminobacteriaceae bacterium isolate AMR_MDS_3026
            NODE_28698_length_3782_cov_3.788302, whole genome shotgun sequence.
ACCESSION   DAOTIL010000172 DAOTIL010000000
VERSION     DAOTIL010000172.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868050
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminobacteriaceae bacterium (wastewater metagenome)
  ORGANISM  Aminobacteriaceae bacterium
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae.
REFERENCE   1  (bases 1 to 3782)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3782)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:19:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,224
            CDSs (total)                      :: 3,198
            Genes (coding)                    :: 3,184
            CDSs (with protein)               :: 3,184
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3782
                     /organism="Aminobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_28698_length_3782_cov_3.788302"
                     /isolate="AMR_MDS_3026"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:3034609"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(92..1285)
                     /locus_tag="P5201_04765"
     CDS             complement(92..1285)
                     /locus_tag="P5201_04765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014163818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Tm-1-like ATP-binding domain-containing protein"
                     /protein_id="HRV97878.1"
                     /translation="MKKAYVVGTCDTKFEELKFVADLIRQSGVEALLVDVGTKPHEHS
                     VDITSREVAACHPSAPSFLDTVKERGEAVGAMGEALAAFLPGRGDVGGVVGLGGSGGT
                     SIVTSGMRALPLGMPKIMVSTMASGNVAPYVGPNDIAMMYSVTDVAGINKVSRVVLAN
                     AANALAGMVKGTVPAGEDRPLLGMTMFGVTTPCVDMVRHALESEFDCLVFHATGAGGQ
                     SFEKLVDSGMMKRVLDVTTTEICDHLMGGVLSAGEDRLGAFARTGIPYVGSVGALDMV
                     NFGAMETVPEKYKNRKLYVHNPQVTLMRTTPEENTLMGEWIGKKLNAMTGPVRFLLPL
                     KGVSMIDAPGQAFYWPEADEALFAALERTVQQSDRRRLVKLPLHVNDPEFAAALVENY
                     REINP"
     gene            1535..2143
                     /locus_tag="P5201_04770"
     CDS             1535..2143
                     /locus_tag="P5201_04770"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRV97879.1"
                     /translation="MNASLMPELLERIQKAFRPIEVRPGAPLPKLPGALPYVKELSGY
                     PRFCGSTMGAEILWNMEILEKAASKPNDPDFSAEYTVAGRHTRVLGRNHRYLDYPVNV
                     FLNICMLHDGQVEIFDFCLNLLAEIAPVNWYAQTLPEEMRGCAIGGKDAYAFDRKNET
                     ITLNVPTAVYKLLPLSLTSASGAVGRVMLPLSSGESFYADRG"
     gene            2228..3259
                     /locus_tag="P5201_04775"
     CDS             2228..3259
                     /locus_tag="P5201_04775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014162472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_component: GO:0031317 - tripartite ATP-independent
                     periplasmic transporter complex [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_function: GO:0005488 - binding [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DctP family TRAP transporter solute-binding
                     subunit"
                     /protein_id="HRV97880.1"
                     /translation="MKKMFGLMCVVALTGLLAGSALAADFTLKLANAGPADPEDRTVI
                     AVEIFKNHVETKTGGSVKVEVFHASQLGNEKEILEGLALGTIELGTITTGPVPTLFKP
                     IMVFDIPFLFPNQVVAWEVLDGPFGRKLMEEMRKSTGIRTLAISENGYRHFVTGSKQV
                     KSMADMKGLKIRTMENPAHMKMVEALGGAPTPIAFGELYMALQQGVVDGCELPVTLIN
                     NGKYYEVQKHVILNGHLYNPLIMFVNDKVWEKLSQDQKAALYEGAQLFNIAQRAISER
                     QVQSGLLNVQKNGMTVYVPTAAELNEFRSAAQPAVLDYVKAQAGEQWVNEALEAVAAA
                     EAKMAEMLK"
     gene            3361..>3782
                     /locus_tag="P5201_04780"
     CDS             3361..>3782
                     /locus_tag="P5201_04780"
                     /inference="COORDINATES: protein motif:HMM:NF016200.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter small permease"
                     /protein_id="HRV97881.1"
                     /translation="MKFLEGVLACFDKAAFAAVALLMGSMGVVAFAAVFYRFVLHDPI
                     TWSEEAARYMMVWVTFLGAGYAMGKGKHIGVTMFVEKLPEGVRRKVIFVAEAVIILFL
                     AAVTVQGVKLIVSLWVQTSPAMDLPMWLPYLAIPVGAV"
BASE COUNT          742 a         1133 c         1147 g          760 t
ORIGIN      
        1 gataatcggc tcgcgccgct tcgccatgtc gtggaacttc gccataagct ccgaacgtgt
       61 cggtcgtttc atcagtgaat ccttcctttc gttacggatt gatctcccgg tagttctcca
      121 cgagcgccgc ggcgaactcc ggatcgttga cgtgcagggg gagcttcacg agacggcgcc
      181 tatcgctctg ctgcacggta cgctcgagcg ctgcgaaaag cgcctcgtcc gcctccggcc
      241 agtagaacgc ctgcccgggc gcgtcgatca tcgaaacgcc cttcagcggg agcaggaagc
      301 gcaccggccc cgtcatggcg ttcagcttct tcccgatcca ctcgcccatc agtgtattct
      361 cttcgggcgt ggtgcgcatc agcgtaacct gcgggttgtg gacgtagagc ttgcggtttt
      421 tgtacttctc cggtacggtc tccatcgcgc cgaagttcac catatcgagc gcgccaaccg
      481 agccgacgta gggaatgccc gtccgggcga aggctccgag gcggtcctcc cctgcgctga
      541 gaacgccgcc catcaggtgg tcgcagatct cggtggtggt gacgtcgagg acgcgcttca
      601 tcatcccgga gtccaccagc ttctcgaagg actgtccgcc cgctccggtc gcgtggaaga
      661 cgaggcagtc gaactcgctt tcgagcgcat gacggaccat gtccacgcac ggcgtggtga
      721 ccccgaacat cgtcataccc agaagcggcc tgtcctctcc cgccggaacc gttcccttca
      781 ccatgccggc gagggcgttg gccgcgttcg ccagaacgac gcgcgagacc ttattgatcc
      841 ccgcgacgtc agtcacggag tacatcatgg cgatgtcgtt ggggccgacg tagggggcaa
      901 cgtttcccga cgccatggtg gagaccatga tcttcggcat ccccaagggc agcgccctca
      961 tgcccgaggt gacgatggat gtcccgcctg agcctcccag cccgacgaca ccgccgacgt
     1021 cgccccggcc cgggagaaac gcggcgagcg cctcgcccat ggcgccgacc gcctcgcctc
     1081 gctccttcac ggtgtcgagg aaggaggggg ccgacgggtg gcacgcggcc acttcgcggc
     1141 tcgtgatgtc tacagagtgc tcgtggggct tggtccccac gtccacgagg agcgcctcga
     1201 cgcccgattg ccgaatgaga tcggccacga acttcagctc ctcgaacttc gtgtcgcacg
     1261 ttcctacaac ataggctttc ttcattccgt cctccttgct gcatcacagg atacccctgt
     1321 ccggggaaaa accacaggga cattataccc ttaacagccc cctgctgtgt gaaaattccc
     1381 gcttccgggg tgatcgagct gcgcgccgga actcttttca cgatgcgcta tcggatgcag
     1441 tacacatttt ggtaactctc tggttattct ttcggtgtac tgctagaata aagaaaagag
     1501 tctgtgccta tcttgctttg caggaggtgt cgtcatgaac gccagtctca tgccggaact
     1561 gcttgaaaga atacagaaag cctttcgtcc gatagaggtc cgtccgggag cgcctcttcc
     1621 caagcttccc ggcgcgcttc cctacgtcaa ggagctaagc ggctacccga gattctgcgg
     1681 ctccaccatg ggcgccgaga ttctctggaa tatggaaatt ctggagaagg cggcttcaaa
     1741 accgaacgat ccggacttca gcgccgagta caccgttgca ggaaggcata ccagagtgct
     1801 cggacggaac caccgctacc tggactaccc tgtcaacgtc ttcctgaaca tctgcatgct
     1861 ccacgacggt caggtcgaga tattcgattt ttgtttaaat ctccttgccg aaatagctcc
     1921 tgtcaactgg tacgcccaga cgctcccgga ggagatgcgg gggtgcgcta tcggaggaaa
     1981 ggatgcctat gcctttgaca gaaagaacga gaccattacc ctaaacgttc cgacggcggt
     2041 ctacaagctt ctgccccttt cgctcacctc ggcgtcgggg gcggtgggaa gagtgatgct
     2101 gcctctctct tcgggggaga gcttctacgc ggatcggggg tgaaacaaca cattcccctt
     2161 tgcaaaggaa aaactttgtt ttataatgcc gggataaatt tatgagagtt tagagggagg
     2221 gtaaaaaatg aaaaaaatgt ttggactcat gtgtgttgtc gctcttacag gacttctcgc
     2281 gggaagcgct ctcgcggccg attttacgct gaagctggcg aacgcggggc ccgccgatcc
     2341 cgaggaccgt acggtcatcg cggtggagat tttcaagaac cacgtcgaaa cgaagaccgg
     2401 cggatcggtc aaggtcgagg ttttccacgc gtcgcagctc gggaacgaga aggagattct
     2461 tgaaggtctg gcgctcggga ccatcgaact gggcactatc accaccggac cggtgccgac
     2521 gctcttcaag cccatcatgg tcttcgacat ccccttcctc ttccccaacc aggttgtcgc
     2581 atgggaagtg ctcgacggcc ccttcggccg caagctgatg gaggagatgc gcaagagcac
     2641 cgggatacgg actctggcga tcagcgagaa cggctaccgc cacttcgtca ccggcagcaa
     2701 gcaggtaaag agcatggccg acatgaaggg gctcaagatc cgcacgatgg agaaccccgc
     2761 gcacatgaag atggtcgagg ccctcggcgg cgcgccgacc cccatcgcct tcggcgaact
     2821 ctacatggcg ttgcagcagg gcgtcgtgga cggctgcgag ctgcccgtga ctctgatcaa
     2881 caacggcaag tactacgagg tgcagaagca tgtgatcctc aacggacacc tctacaaccc
     2941 gctgatcatg ttcgtcaacg acaaggtttg ggagaagctc tcccaggacc agaaggccgc
     3001 gctctacgag ggcgcgcagc tcttcaacat cgcgcagcgc gcgatctccg agcggcaggt
     3061 gcagagcggc ctgctgaacg tgcagaaaaa cggaatgacc gtctacgttc ccacggccgc
     3121 cgaactgaac gagttccgtt ccgcggcgca gcccgccgtg ctcgactacg tcaaggcgca
     3181 ggcgggagag cagtgggtga acgaagcgct tgaagctgtc gccgcagccg aggcgaagat
     3241 ggcggagatg ctgaagtagc ttccggacgg attcttttat cgtcgggaat acacatcggg
     3301 gaaggggcgc gttgttgccc cgtccccttt tccatttcac tgtgaaggag aggtggagca
     3361 atgaagtttt tggagggagt attggcgtgc ttcgacaagg cggccttcgc cgcggtcgct
     3421 ctcctgatgg gaagcatggg cgtcgtcgcg ttcgcggcgg tcttctaccg ctttgtcctt
     3481 cacgacccca tcacgtggtc cgaggaggcc gcgcggtaca tgatggtgtg ggtgaccttc
     3541 ctgggggccg gatacgcgat ggggaagggg aagcacatcg gcgtcacgat gttcgttgaa
     3601 aagcttccgg agggggtgcg gcggaaggtt atcttcgtgg cggaggctgt gatcatcctc
     3661 ttccttgccg cggtgaccgt acagggtgtg aagctcatcg taagcctgtg ggtgcagacg
     3721 tcgcccgcga tggaccttcc catgtggctg ccgtatctgg cgatccccgt gggcgccgtg
     3781 ta
//
LOCUS       DAOTIL010000173         3773 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminobacteriaceae bacterium isolate AMR_MDS_3026
            NODE_28792_length_3773_cov_3.923346, whole genome shotgun sequence.
ACCESSION   DAOTIL010000173 DAOTIL010000000
VERSION     DAOTIL010000173.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868050
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminobacteriaceae bacterium (wastewater metagenome)
  ORGANISM  Aminobacteriaceae bacterium
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae.
REFERENCE   1  (bases 1 to 3773)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3773)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:19:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,224
            CDSs (total)                      :: 3,198
            Genes (coding)                    :: 3,184
            CDSs (with protein)               :: 3,184
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3773
                     /organism="Aminobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_28792_length_3773_cov_3.923346"
                     /isolate="AMR_MDS_3026"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:3034609"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..909)
                     /locus_tag="P5201_04785"
     CDS             complement(<1..909)
                     /locus_tag="P5201_04785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013047502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter substrate-binding protein"
                     /protein_id="HRV97882.1"
                     /translation="MKLKGMVVMALACAMVFSLVTFGEAAKYEFKLGHAVSDQHPYHL
                     GAQKFKEIVEKETNGEIEISLFPNNQLGTGERDLLEGLQLGTVDLYVGSTGPMGGFEK
                     RFMMFDFPFLFKDKAQAYAVLDGKIGQYVMGLLDRIGITGLAWYENGFRHFTNSKRPV
                     NSPEDVKGLKLRTMENKIHMALWRAMGADPTPMAWGEVFTALQQGTVDGQENPVPIIF
                     VQKVYEVQKHLALTGHVFSPAMIAIAKAKFDALPEVHKEIIRKAAQESALFQREQITK
                     MESEQVAKLKELGMEVTTPDVTPFREA"
     gene            complement(956..1888)
                     /locus_tag="P5201_04790"
     CDS             complement(956..1888)
                     /locus_tag="P5201_04790"
                     /EC_number="1.1.1.169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013047501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008677 - 2-dehydropantoate 2-reductase
                     activity [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydropantoate 2-reductase"
                     /protein_id="HRV97883.1"
                     /translation="MKTVILGSGAMGSLYGGMLAEAGFDVTLVDVWKEHIDAVNASGL
                     SIEGIGGDRVIRSIRGTTDPAQAGKADLVIVFVKATATAQAMKGALSLLGPETVVLTL
                     QNGLGNVEKLNETAGAERVMAGITGHGCTLLGPGKIRHAGQGDTILGEPRGQISDRLK
                     RVGGMFEKAGFSVKLSENVIGLIWGKLFVNVGINALTAVTGLKNGRLVELQETEELLE
                     LAVREALEVAQAAGIRIETENPVEHTKKVARLTAANRSSMLQDVTNRRQTEIDVINGA
                     IVNEGKKLGVPTPVNLALTNLVRVRQKTYDEFSV"
     gene            complement(2024..2803)
                     /locus_tag="P5201_04795"
     CDS             complement(2024..2803)
                     /locus_tag="P5201_04795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013047499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IclR family transcriptional regulator"
                     /protein_id="HRV97884.1"
                     /translation="MEGKQRATGGVQSIERAFAIIELLDAQGELGIAEMSRLLSLERS
                     TVHRIVSTIKNLGYINQNPLNHKYSNSFKFFEIGNDVVKRLGLRQQAAPFLRELSEKT
                     HEAVNLAILDGDRVIYIDKIESQSTIKVDLSVGKRFPAYCTGLGKILLAWLPEPKILE
                     IVGPSPFPRYTENTVTSTEDLLAQLREIRDREIAWDNEEYVEGLFCIAAPVRGNSGDV
                     VAAMSVALPKFLYAGESRKIEAVGDLLLDVARRFSRSLGYK"
     gene            complement(3160..3753)
                     /locus_tag="P5201_04800"
     CDS             complement(3160..3753)
                     /locus_tag="P5201_04800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013048918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclodeaminase/cyclohydrolase family protein"
                     /protein_id="HRV97885.1"
                     /translation="MAADSAAPGGGSVAALSGALGAALVSMVCRLTIGKKGYEEYELE
                     VKDALAESEVLRKRLLEAIDIDAKAFEKVMEAFAAPKSTDEEKAARREMIQTAFKGAC
                     ESPRRTAADCLEVLRLCARVADKTNTNAASDLGVGASNALAALESAAMNVLINLPSIK
                     DDEYVDELRDEMEAIQEEGGILRTGVLREVSAHIGGN"
BASE COUNT          790 a         1163 c          958 g          862 t
ORIGIN      
        1 cgcctcacgg aagggagtca cgtcgggcgt cgtcacttcc atgccgagct ccttcagctt
       61 ggcaacctgt tcgctctcca tcttcgtaat ctgctctcgc tggaagagcg ccgattcctg
      121 cgcagcctta cggatgatct ccttgtgtac ttcgggaagc gcgtcgaact tcgccttcgc
      181 gatggcgatc atcgcgggag agaagacgtg acctgtaagc gcaaggtgtt tttgcacctc
      241 gtacaccttc tggacgaaaa taatgggcac cgggttctcc tgtccgtcga ccgtcccctg
      301 ctgcagcgcg gtaaaaacct cgccccacgc catcggcgtc gggtcggctc ccatggctct
      361 ccagagcgcc atatggatct tgttctccat ggtccgcagc ttgagcccct tcacatcctc
      421 gggggagttc acaggccgtt tggagttggt gaaatggcgg aacccgttct cgtaccacgc
      481 aagaccggtg atgccgatcc tgtcgaggag ccccatgacg tactgcccga tcttcccgtc
      541 gaggacagcg tatgcctgtg ccttgtcctt gaagaggaac gggaagtcaa acatcatgaa
      601 gcgcttctcg aagccgccca tcggccctgt cgatcctaca tagaggtcga ccgttccgag
      661 ctgaagccct tccagcagat cccgctcccc cgtgccaagc tggttgttcg ggaaaaggga
      721 gatctcgatc tcgccgttgg tctccttctc gacaatctcc ttgaatttct gcgctcccaa
      781 atgatacgga tgctgatcgc ttacggcgtg tccgagcttg aactcgtact tcgccgcctc
      841 cccgaacgtc acgagcgaga aaaccatcgc acacgccaac gccatcacaa ccatgccttt
      901 caacttcata ttccttcccc tccttacgtg aattggacgt ctcccccgcg gtaagttaca
      961 cggaaaattc gtcataggtc ttctgacgga cccttacgag gttcgtcagc gccaagttga
     1021 cgggagtggg cacccccagc tttttccctt cgttcacgat cgcgccgttg atcacgtcga
     1081 tctccgtctg cctccggttt gttacgtcct gcagcatcga ggaacggttc gccgccgtca
     1141 ggcgcgccac cttttttgtg tgctcgacgg ggttctccgt ctcaatgcgt atccccgctg
     1201 cttgcgctac ttccaacgcc tcccgaacgg caagctccag cagctcttcg gtttcctgaa
     1261 gctcgacgag tctgccgttc ttgaggccgg tcacggcggt caaggcgttg attccgacat
     1321 tcacaaacag cttcccccaa atgagtccga tgacgttctc ggagagtttc acggaaaaac
     1381 ccgctttttc aaacatcccg ccgactctct ttaacctgtc gcttatctgc ccgcgaggct
     1441 cgccaaggat cgtatccccc tgccccgcat ggcgtatctt tccgggaccg agaagcgtac
     1501 agccatgccc cgtgattccg gccataaccc gctcggctcc cgctgtttcg ttcagtttct
     1561 ccacgttacc gagaccgttc tggagcgtaa gcacaacggt ctccggcccg agaagcgaca
     1621 gagcgccctt catcgcctgc gctgtagcgg tcgctttcac gaagacgatg acgagatcgg
     1681 ctttccccgc ctgcgcagga tcggtcgtgc ctcgaatgga gcgaataacg cggtcgccgc
     1741 cgatgccttc tatcgaaagc cccgatgcgt tcaccgcgtc gatatgctcc ttccagacgt
     1801 cgacaagcgt tacgtcgaat cccgcttcgg cgagcatgcc gccgtaaaga gaacccattg
     1861 cgcctgaacc caggataacg gtcttcattt tctcctccta cttgaattcg ttcttcttcc
     1921 ctagaggacg gagtgtgcga tacgcacatc cttccgaatt gcctctctaa ccgggcggac
     1981 agctccataa aaaaaccgtc tcatatgagg acgacgtcgt agattattta tacccaagtg
     2041 aacgggagaa tcttctcgcg acatccagca acaaatctcc taccgcttca atcttacggg
     2101 attctccagc gtagaggaat ttcggaagcg caacgctcat cgcggccacg acatccccgg
     2161 aattaccccg taccggcgcg gcgatacaga agagaccctc gacatactcc tcgttgtccc
     2221 aggctatctc gcgatccctg atctcccgga gctgagcgag cagatcttca gtcgatgtca
     2281 cggtgttctc cgtatacctt gggaagggag atgggcctac aatctcaaga atcttcggct
     2341 ccggaagcca cgcgagaagg atctttccaa gtcccgtaca gtacgcaggg aagcgcttcc
     2401 ccacagaaag atccaccttg atggtagatt ggctttctat cttgtcgatg tagatcaccc
     2461 tgtcgccgtc gagtatggcg agattcaccg cctcgtgcgt cttctcggaa agttcccgga
     2521 gaaagggagc ggcctgctgg cgaagaccga gccgtttgac aacatcgttg ccgatctcga
     2581 agaatttaaa gctgttcgaa tacttgtgat tgagggggtt ctgattgatg tagccgagat
     2641 ttttgatggt ggacacaata cgatggaccg tactgcgctc caaggaaagc agacggctca
     2701 tttcagcaat tccaagctct ccctgagcgt ccagaagctc gataatcgcg aaagcgcgtt
     2761 ctatggattg gactccgccc gtggccctct gcttcccttc catacttttt cctccatacc
     2821 atccgagaga atctcgcgtt tcatttcccg cattacggaa tgatattgat tatagtggaa
     2881 ttgatcttgc ggatcattat aataaccggt tctttctcat gtcaatagaa aaagagcgca
     2941 attccgccaa gacagtaagt tcttttttta tcaattatga cgcggcaaaa gggagaagag
     3001 cgttgcgacg gatactacga aagggaaaaa gacagatccg gtttggaaaa tatgggacag
     3061 gggcgacacg caacaacaac cgccgttttg acgttcgaaa ggaaaaggac cgcgtcagca
     3121 caaagcgcgg tcctttaaaa cccgacgaat ccgaaacgat tagttcccgc cgatatgcgc
     3181 cgatacttcg cgcaagactc cggtccgcag gataccgcct tcctcctgga tggcctccat
     3241 ttcgtcgcgc aactcatcga cgtactcatc gtccttaatc gaaggaaggt taatcaagac
     3301 gttcatagcg gcgctttcga gggcggcaag cgcattggac gctcccactc cgagatcgct
     3361 tgctgcgttc gtattcgtct tgtcggcgac ccgagcgcac agacgaagca cttcaaggca
     3421 atccgcagcc gtccggcgcg gcgactcgca ggcgcccttg aaagcggtct gtatcatctc
     3481 ccggcgcgca gccttctcct cgtcggtact cttgggcgcg gcaaatgcct ccataacctt
     3541 ttcgaacgcc ttcgcgtcga tatcgatcgc ttccagaaga cgcttccgga gaacctcgga
     3601 ttcggcgagc gcatccttca cctcgagctc gtactcttca tacccctttt tgcctatggt
     3661 aaggcggcag accatcgaga ccagagcggc cccgagagct cctgagagcg cggcgacgct
     3721 gccgcctccg ggtgcggcgg aatcggcggc cagttcgtcg gtaaatccac caa
//
LOCUS       DAOTIL010000174         3770 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminobacteriaceae bacterium isolate AMR_MDS_3026
            NODE_28822_length_3770_cov_3.171467, whole genome shotgun sequence.
ACCESSION   DAOTIL010000174 DAOTIL010000000
VERSION     DAOTIL010000174.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868050
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminobacteriaceae bacterium (wastewater metagenome)
  ORGANISM  Aminobacteriaceae bacterium
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae.
REFERENCE   1  (bases 1 to 3770)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3770)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:19:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,224
            CDSs (total)                      :: 3,198
            Genes (coding)                    :: 3,184
            CDSs (with protein)               :: 3,184
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3770
                     /organism="Aminobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_28822_length_3770_cov_3.171467"
                     /isolate="AMR_MDS_3026"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:3034609"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..615)
                     /locus_tag="P5201_04805"
     CDS             complement(<1..615)
                     /locus_tag="P5201_04805"
                     /inference="COORDINATES: protein motif:HMM:NF014647.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BMP family protein"
                     /protein_id="HRV97886.1"
                     /translation="MKKFVALLALCLLVLFAGTAAADVKEYKDFKLAAVFQTAIEEPW
                     DGAIHQACLKVQKETGITYEFAEKVGAADFERVLREYAERGFDLIVGDAFLAGEEPSR
                     RVAKEYPEVAFAFGSEFTFQEPNYSVFDNWIHEPSYLCGVIAGRMTKSNTLGIVAAIP
                     IAEVNRLVHAFKEGALSVNPAVKVKVAYIGSWFDPPKAKEAALAQ"
     gene            949..2229
                     /locus_tag="P5201_04810"
     CDS             949..2229
                     /locus_tag="P5201_04810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006301715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytosine deaminase"
                     /protein_id="HRV97887.1"
                     /translation="MDLVVRNARLRGESGTKDIAVRDGLVAAIGERLAVQAGREIDAG
                     GNLASAPFIDPHLHLDAALSVGDPRYNESGTLLEGIAIWGERRPSLQKNEVLRNAREA
                     VLWEVANGVQFIRTHADTTDSALVTVEALVELREEMKDVAEIQVVAFPQDGVFTFPEG
                     EANLRRALDMGCDCVGAIPHIEFTREDGVRSVELAFELAEQYGRPVDIHCDETGDDQS
                     RFVEVMAKLAIATGMGSAVTASHTTAMHNYNNDYALKLMGIFRRAKMNFITAPFDNSV
                     LQNRTDGYPRRRGHTRVDELDAAGVNVSIGHDSIMDPWYPMGRGSMLAAANLLMHTAH
                     MSGREQISRLFDMITDNSAVTMGVRDRYGVTPGAPADFVILDAPDEFEALRLQSECLF
                     VVRGGTVVCETAPAKRRLRRSGAVREVDFKLPAR"
     gene            2464..2889
                     /locus_tag="P5201_04815"
     CDS             2464..2889
                     /locus_tag="P5201_04815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012869150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030151 - molybdenum ion binding [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MOSC domain-containing protein"
                     /protein_id="HRV97888.1"
                     /translation="MAKIIALCTSSQRQEPKSEVAEAHCVHGGIEGDAHRGVTEREIS
                     LLRAEDIRRAEQEAGFVFPPGSLAENLVVEGLPEDLAPGARLSIGPEAVLEVVEKGKK
                     PGEPHSYSYKGWCLLPTAGFFLRVIEEGVVSKGDEVESL"
     gene            2970..>3770
                     /locus_tag="P5201_04820"
     CDS             2970..>3770
                     /locus_tag="P5201_04820"
                     /inference="COORDINATES: protein motif:HMM:NF024140.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MCP four helix bundle domain-containing protein"
                     /protein_id="HRV97889.1"
                     /translation="MFRKMKIGGKLSLAFGVLLLIFAGVGAMSWMNMSGVQQEARSLA
                     ERYVPELVVAANVQNTVQTMMYEVRGYNFTYSKSYLDAGRNAVVEARKALKEASDLGE
                     KYPTLVALREGAAKAVAALDEYVVLIDRTETAVNAIDGARTSGNESARLFMENAEAYL
                     ASQNQAMLHALRNNEPREETEDRLQKITLANEIIDAGNAVRLANYMGQATRNVVRFEE
                     GMKNFDEIDSLVKKLRADTAQKVNLDQLNAVQKAAGEYKTAMEKTLASW"
BASE COUNT          780 a         1096 c         1191 g          703 t
ORIGIN      
        1 ctgggccagg gcggcttcct tcgccttcgg aggatcgaac cagctgccga tgtaggcgac
       61 cttcaccttg acggccgggt tcaccgagag cgcgccctct ttgaatgcgt gtaccagcct
      121 gttgacctcg gcgatgggga tggccgcgac gatgcccagc gtattgctct tcgtcatccg
      181 tccggcgatc accccgcaca ggtaggaggg ctcgtggatc cagttgtcga agaccgagta
      241 gttcggctcc tggaaggtga actccgagcc gaaagcgaac gcgacctcgg ggtactcctt
      301 cgccacgcgc cgcgaaggct cctcgcccgc gaggaatgcg tcgccgacga tcaggtcgaa
      361 ccctctctcg gcgtactcgc ggagcacgcg ctcgaagtcc gccgcgccga ccttctccgc
      421 gaactcatag gtgatgccgg tctctttctg caccttcagg cacgcctgat ggatcgcgcc
      481 gtcccacggc tcctcgatcg ccgtctggaa gacggccgcg agcttgaagt ccttgtactc
      541 cttcacatcg gccgccgccg ttccggcgaa gagaaccagc agacaaagag caagcagagc
      601 tacaaatttc ttcacacaaa tgcacctccc gttcagagta tccggcagac gggcggatgc
      661 ccgacgccgt cgtacagacc atgaattccc acgttccgca tggaagagtt ccgccccgtc
      721 acctccttga cctccgcggt ttttcctaat cgcgccaatt cccattttat cagaagttct
      781 gcactctcga tgattttcaa tcattcaatc aacgagaggt tcttctgtca atggcgacgg
      841 gtgacgtgaa tattcgctct tcctgtcggc agtggacagg gaagagcgat tgtggtacta
      901 ttttaatgca aacgattgca taaatcaaat cggcggggag aagagagaat ggaccttgtg
      961 gtacgcaatg cgcggcttcg cggagagtca ggaacgaagg atatagcggt tcgtgacggc
     1021 ctggtggccg cgatcggaga acggctcgcc gttcaggccg gccgtgagat cgacgccggg
     1081 ggcaacctgg cgtcggcgcc cttcatcgac cctcatctgc acctcgacgc cgccttgagc
     1141 gtgggagatc cccgttacaa cgaatcggga acgctcctcg aaggcatcgc catctggggg
     1201 gagcgccgcc cctctcttca aaagaatgaa gtgctccgca acgcgcgcga ggccgtgctc
     1261 tgggaggtcg ccaacggcgt gcagttcatc agaacccacg cggacaccac cgattccgcc
     1321 ctcgttacgg tggaggcgct cgtcgagctg cgcgaggaga tgaaggacgt cgcggagatc
     1381 caggtggtcg cctttccgca ggacggcgtc ttcaccttcc cggagggtga ggcgaacctt
     1441 cgccgcgctc tcgatatggg gtgcgactgc gtcggcgcca tcccgcacat cgaattcacc
     1501 cgcgaggacg gcgtccgctc ggtcgagctg gccttcgagc tggcggagca gtacggccgc
     1561 ccggtggata ttcactgcga cgaaaccgga gacgaccagt cgcgcttcgt cgaggtcatg
     1621 gcgaagctcg ccatcgcgac ggggatggga agcgccgtca cggcgagcca taccacggcg
     1681 atgcacaact acaacaacga ctacgcgctc aagctgatgg ggatcttccg gcgggcgaag
     1741 atgaacttca tcaccgcgcc gttcgacaac tccgtgctcc agaaccgcac ggacggctat
     1801 cctcgccgca gggggcacac gcgggtggac gaactggacg ccgcgggcgt caacgtctcc
     1861 atcggacacg actccatcat ggacccctgg tacccgatgg ggaggggctc catgctcgcc
     1921 gcggcgaacc tgctgatgca cacggcgcac atgagcgggc gcgagcagat cagccgactt
     1981 ttcgacatga tcacggacaa ctccgccgtg accatgggcg tccgggatcg ctacggcgtc
     2041 acaccgggcg ctccggccga ttttgttatc ctcgacgctc ccgacgaatt cgaagcgctt
     2101 cgtctccaga gcgaatgcct cttcgtggtg cgcggcggta cggtggtgtg cgagactgcg
     2161 cctgcaaagc ggcggttgcg ccggagcggc gccgttcgcg aggtcgattt caagctgccg
     2221 gcgaggtgat gtgaaggttt ttcgcaagag ccggacgctc gaagcccgat gtccgaagcc
     2281 cgagaggacg tcctccgggt gtatcgcaat gatattgcgg agaaaaagcg gtcttgccaa
     2341 aaaggtaagg ccgcttttga gtcttttccg ggtgggcagg gggtcgtatt tttccctttc
     2401 atcttgtatt attcaataga gtcaatgttt cgttatcctg ttgccgatgg aggaatggtt
     2461 attttggcga agatcattgc actgtgtaca agttcgcagc gacaggaacc gaagagcgag
     2521 gttgcggagg cgcattgtgt tcatggaggc atcgaaggag atgctcacag aggagtgacc
     2581 gagagagaga tcagccttct tcgcgccgag gacatccgga gggcggagca ggaggccgga
     2641 tttgtcttcc cccccggatc gcttgcggag aatctcgttg tggaagggct tccggaggat
     2701 cttgcgccgg gagcgcgtct ctcgatcggc cccgaggccg tcctcgaagt cgtggagaag
     2761 ggaaaaaagc cgggcgagcc gcacagctac agttacaaag ggtggtgtct gctgccgacg
     2821 gcggggttct ttcttcgcgt gatcgaggaa ggtgttgtct cgaaggggga cgaagtcgaa
     2881 tccctttgaa gcgcgtgcct ttctgaatga gaaaccgttg gatctgaaac aatcgtattc
     2941 attatttcac ggaaggagag gtggatgcag tgtttcgaaa gatgaaaatc ggagggaaac
     3001 tctcgctcgc gttcggcgtt ctgctgctca tattcgcggg agtcggcgcg atgagctgga
     3061 tgaacatgag cggcgtgcaa caggaagccc gctctctcgc ggagcggtac gttcctgaac
     3121 tggttgtcgc tgccaacgtg cagaatactg tgcagaccat gatgtacgaa gtccgaggat
     3181 ataacttcac ctactccaaa agctacctcg acgcggggcg gaacgctgtc gtcgaagcgc
     3241 ggaaggcgct gaaagaggcg tccgacctcg gggagaagta cccgacgctc gtcgcgctcc
     3301 gggagggtgc ggcgaaggcc gtcgcggcgc ttgacgagta cgtcgtcctg atcgacagga
     3361 cggagacggc ggtgaacgcc atagacggtg cgcgaaccag cgggaacgaa agcgcgcgcc
     3421 tcttcatgga gaacgccgag gcctaccttg cgtcccagaa ccaggccatg ctccacgctc
     3481 tccggaacaa cgaaccgcgc gaggaaacgg aagacaggct gcagaagatc acccttgcaa
     3541 acgagatcat cgacgcaggg aacgccgtgc gccttgcgaa ctacatgggg caggcgacca
     3601 ggaacgtcgt ccgattcgag gaggggatga aaaatttcga cgaaatagat tccctcgtca
     3661 agaagctccg cgccgacacg gcgcagaagg tcaacctgga tcagctcaac gccgtgcaga
     3721 aggctgcggg cgagtacaaa accgcaatgg agaaaactct cgcgagctgg
//
LOCUS       DAOTIL010000175         3760 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminobacteriaceae bacterium isolate AMR_MDS_3026
            NODE_28932_length_3760_cov_3.002159, whole genome shotgun sequence.
ACCESSION   DAOTIL010000175 DAOTIL010000000
VERSION     DAOTIL010000175.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868050
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminobacteriaceae bacterium (wastewater metagenome)
  ORGANISM  Aminobacteriaceae bacterium
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae.
REFERENCE   1  (bases 1 to 3760)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3760)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:19:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,224
            CDSs (total)                      :: 3,198
            Genes (coding)                    :: 3,184
            CDSs (with protein)               :: 3,184
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3760
                     /organism="Aminobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_28932_length_3760_cov_3.002159"
                     /isolate="AMR_MDS_3026"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:3034609"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..541
                     /locus_tag="P5201_04825"
     CDS             <1..541
                     /locus_tag="P5201_04825"
                     /inference="COORDINATES: protein motif:HMM:NF014234.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004665 - prephenate dehydrogenase (NADP+)
                     activity [Evidence IEA];
                     GO_function: GO:0008977 - prephenate dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_process: GO:0006571 - tyrosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="prephenate dehydrogenase/arogenate dehydrogenase
                     family protein"
                     /protein_id="HRV97890.1"
                     /translation="PRYAGFHPMAGKEKGGIGNADPELFLGSICAIVPFPGTSPSVLT
                     LAEELASALGGRSLRTDPAEHDAVTACISHFPVLVAASLSLLAGKEREIHPLLPLLAA
                     GGFRDTTRVAGGPAWLGADMASTNGVQVRRLAEEFKNVLDELLSASPEELEILLAKAS
                     ECRESILAAKEAWRKEERQ"
     gene            1441..3303
                     /locus_tag="P5201_04830"
     CDS             1441..3303
                     /locus_tag="P5201_04830"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRV97891.1"
                     /translation="MGAKPRRSFAETLYSLPLLGYCAELLVQLVRLPVVLREVNIRQT
                     RKDTVVQSRLDGIGNRVAALREGASLCRREMEAALSGKADADTLASISEDLSKKVDAE
                     RFDSFTLNLEAELSRKVSAESLEDLGASWETALSELREELSGKAGKDSLRAMENELQT
                     KANAGSTAAIEEKVERIIERLGALDGSAVLDRLESMELLLEGKADLEEVAKLLSAKLD
                     TDALEIWGKRLEDASAQLLEALSQKADVERVDALSESLRSKADGETLESLSAAKADVE
                     ELLSLKARFESLGAHTSELENTLHSLCASKADLDAVRSLGERFEGVAAQLGALEGESV
                     LKRLSSAESAIERKADLEIVKSLLSLKMDVEVLKSLGKSLEEASGQTFAALEKKADLH
                     SVESVASTLDLKADAESVRSLNEGLSVVNERLGDFASKEDFSKVGELERLLSRKADAD
                     ALEERLSALDAKADLKTVEEKLAQKADAEAVESLSKEFEQRLAVKADAAAVDEALAKK
                     AGKDALDEVQKSVGSELSGLHARIDEGREQASEEERRLVRFREEVKALIASSLERGAR
                     GQIEEAFRATFPYVQKMKGRFPAFQGIDTQIKKTTQRSGGQGTEDAQSRKRVKR"
     gene            3300..>3760
                     /locus_tag="P5201_04835"
     CDS             3300..>3760
                     /locus_tag="P5201_04835"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRV97892.1"
                     /translation="MSKRIALVTADVHGEERWGEDRFLEGLRTALESKGMTAEIVAVL
                     HDESSYEALSDTYARYAALDLSSFDGVVSTTIPGCAAVHPNKVCHLMRTARRFYDMFD
                     TEHPSPSPEHRQLRRHLQELDRAAFKDLDTVFVASGEIRGRLLKYLGVDSEV"
BASE COUNT          806 a          992 c         1153 g          809 t
ORIGIN      
        1 accgcgatac gcggggtttc accccatggc ggggaaggag aaggggggga tcggtaacgc
       61 agaccccgaa ctcttccttg gttccatctg tgccattgtc cccttccccg ggacctcccc
      121 ttccgttctt accctggcgg aggaactcgc ttcggcgctc ggggggcgtt ctctccgaac
      181 agatccggcc gaacacgacg cggtgactgc ctgtatcagc cactttccgg tgctcgtggc
      241 tgcatcgctc tccctgcttg ccggaaagga acgggagatc cacccgctcc tgccgcttct
      301 tgcagcggga ggttttcgtg ataccacgcg cgttgccggc ggccccgcat ggctcggcgc
      361 ggacatggcg agcaccaacg gcgtacaggt gcgccgcctg gcggaggagt tcaaaaacgt
      421 attggacgag ctgctctccg cttctccgga agagctggag attttgctgg cgaaggcttc
      481 ggagtgccgc gagtcgatcc ttgctgcgaa ggaagcatgg agaaaagagg agagacagta
      541 aatttcgtca aagcagcagc gcctcttctt tctttcgtca acactatggg cgttgaatag
      601 agtgccggga gaaacagctt cgtttgatac cgggtttcgc cggcatgagg agggatctct
      661 cctctccagg agaattcaca ggcgccttcc gctgattcac gcgaagcttg ttcctctcgt
      721 atcgtgtttc tcgccgtcgg acgaacgtat caggtagcga acttctcccc gaggagcgtg
      781 aaaagaggag ctcctttgga gaattgagct ccccttgctt tccaagtgtt ggcttcttgt
      841 gaagtctatg atactcttga acagtgttca agccattcat tcagaaggtt accgcttttc
      901 ggagggagcc gccgcgtgga aaggagaatt tcgggagtga acgaaaaaaa gaatactatt
      961 ttttttgatg ggggcggcga gccatctcct gctgttcttg atgaagagag cgctctgctt
     1021 ttttcgtctc ttccccgaag ctcctcggac gcagggagac cgaatgtctc cggcgattcc
     1081 caatctgcgc ctctccccga tacccggacg gatacgactt ccgaactgcc cgtcgtcata
     1141 cggcgggaac ccgcgagttc cccgaatgct gcggacaatt ctcttcctcc gtccggacag
     1201 agcgctcaac tgcaaaaagg gcaggctctg attgaacgtc tccgtcatga acttgccgta
     1261 cggcggcggg aagattctgt atatccttca acgcgccgcc cccgcctgga aaagaaggcg
     1321 ggccgggttt ctccgcagac agcttccgtc cactctcaac ccccgaaaac agctcctgtg
     1381 caagcgaaac gcgcagctcc ctcttggaac tcacggaaat ctgaagggaa gccatccttt
     1441 gtgggcgcca agccccggcg ttcctttgcg gaaacactgt actcgttacc tctgctcggt
     1501 tactgtgccg aactccttgt ccagctagtc aggcttcccg ttgttctccg cgaggtgaat
     1561 atccggcaga ccaggaagga tactgttgtg cagtcgcgac ttgacggtat agggaacagg
     1621 gtcgccgcgc tccgggaggg agcttcgctc tgccgccggg aaatggaggc ggcgctctcg
     1681 ggcaaagcgg atgccgacac tttggcttct atctcggagg atttgtcaaa gaaagttgac
     1741 gcagagcggt ttgacagctt taccttgaat cttgaggcgg aattgtctcg aaaggtttcc
     1801 gcggagtctc ttgaggattt gggcgcttca tgggagacgg ctttgagcga gctcagggag
     1861 gaactttccg ggaaagccgg caaggacagc ctgcgcgcaa tggaaaacga gttgcagacg
     1921 aaggcgaatg ccgggagtac agcggcgatt gaggagaaag tggagcgcat tattgagcgt
     1981 ctgggcgccc tcgacggaag tgcggttttg gatcgtctgg aatccatgga attgcttctc
     2041 gaaggaaaag cggatcttga ggaagtcgcg aagctgcttt cagcaaagct cgatacggac
     2101 gctctggaaa tctggggaaa acgcctggag gacgcttccg cccagttgtt agaagcgctt
     2161 tctcaaaagg ctgacgtgga gagagtggat gcgctttccg agtcgctgcg ctcgaaggcg
     2221 gacggagaaa cgctcgaatc gttgtctgcg gcaaaagcag atgttgaaga gcttctctct
     2281 ttgaaggctc gtttcgaatc cctcggcgca cacacctccg aactcgagaa taccttgcat
     2341 tctctttgtg cgtcgaaagc ggatttggat gctgtgcgtt ctcttggcga gcgtttcgaa
     2401 ggagtagccg ctcagcttgg cgcgctcgaa ggagagagcg tcttgaaacg attgagctcg
     2461 gcggagtcgg caatcgaacg taaggctgat ctggagattg tgaaatcgct gctctcgctc
     2521 aagatggacg tcgaggtgct gaaatcgctt ggaaaaagcc tggaggaggc gtccggccag
     2581 acgttcgccg cacttgagaa aaaagcggat cttcacagcg tcgagtctgt ggcctccact
     2641 cttgatctga aagcggatgc ggagtcggta cgctccttga acgaggggct ttcggttgtg
     2701 aacgaacgtc tcggcgattt tgcttccaaa gaggattttt caaaggttgg ggagttggag
     2761 cggctgcttt ccaggaaggc ggacgctgat gcgcttgagg aaagactctc tgcgcttgac
     2821 gcgaaagccg acctgaagac ggtggaggag aagctcgcgc agaaagccga cgctgaagct
     2881 gttgaatcac tctcgaagga atttgaacaa cgtcttgccg taaaagcgga tgccgcagcc
     2941 gttgacgagg ctcttgcgaa aaaggccggg aaagatgcgc tggatgaagt ccagaagtct
     3001 gtcgggagcg agctgagcgg cctgcacgcc cgtatcgatg aggggcgaga acaagcgagc
     3061 gaggaagaac gcagacttgt tcgcttccgc gaagaggtga aggcgctcat cgcttcctcc
     3121 ctcgaacggg gagcgcgcgg gcaaatcgaa gaagcgttcc gcgctacctt cccctatgta
     3181 caaaagatga aggggcgctt tcccgcgttt caagggattg atacgcaaat aaagaagacg
     3241 acacaacgtt ccggcgggca ggggacggaa gatgcacaat ccaggaagag ggtgaagcga
     3301 tgagcaagcg tattgctctc gtaacggccg atgttcacgg tgaagaacgg tggggagaag
     3361 accgttttct ggaggggctc cgtaccgctc ttgaatcgaa gggaatgacc gcggagatag
     3421 tggccgttct tcacgatgaa tcatcgtacg aggctctgtc ggatacgtat gcgcggtatg
     3481 ctgcgcttga tctttcctcc tttgacggcg tcgtcagcac cacgattccc ggatgcgccg
     3541 ccgtccaccc gaacaaggtc tgtcacctta tgcgaacggc acgacgtttt tacgacatgt
     3601 tcgatacgga acacccttcc ccctccccgg agcacagaca gttgcgccgc catctgcagg
     3661 aactggatcg tgccgcgttc aaggatctgg acactgtttt cgtcgcaagc ggcgagattc
     3721 ggggacgact gctgaagtat ctcggcgtcg attcggaggt
//
LOCUS       DAOTIL010000176         3756 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminobacteriaceae bacterium isolate AMR_MDS_3026
            NODE_28972_length_3756_cov_2.943529, whole genome shotgun sequence.
ACCESSION   DAOTIL010000176 DAOTIL010000000
VERSION     DAOTIL010000176.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868050
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminobacteriaceae bacterium (wastewater metagenome)
  ORGANISM  Aminobacteriaceae bacterium
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae.
REFERENCE   1  (bases 1 to 3756)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3756)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:19:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,224
            CDSs (total)                      :: 3,198
            Genes (coding)                    :: 3,184
            CDSs (with protein)               :: 3,184
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3756
                     /organism="Aminobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_28972_length_3756_cov_2.943529"
                     /isolate="AMR_MDS_3026"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:3034609"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            476..2041
                     /locus_tag="P5201_04840"
     CDS             476..2041
                     /locus_tag="P5201_04840"
                     /inference="COORDINATES: protein motif:HMM:NF012711.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HRV97893.1"
                     /translation="MICLKRSDALALISKERSVTYAGLCDMISRYSALISSTPCTKVA
                     IFSENRPEWVYIMYAGWKSGYTVVPIDYMSPADEVAYILADCKPEKIFCSRRKFPELE
                     KALLSLSYAPEILVFEECLLPPGANASGDHFLERDDDDTALIIYTSGTTGSPKGAMIS
                     FANIAANVRAVSEAAPIFTPESRVLVLLPIHHVLPLGGTILGPLFVGACCVFSPSIGS
                     RDILETLQHNGVTVVVAVPRFYSMLIKGIREKIQESIAARTFFALAKKIDHPGLSKFL
                     FKKVHERFGGKIRFLVCGGAAIDDDVARDFKALGFDMLTGYGMTETAPLISFTHPGTL
                     RIGASGYVCPAVEVRIVDGEITVRGRHVMKGYYNRPEETAAMIRDGWLHTGDLGFVDE
                     DGFLFVTGRKKDIIVLSNGKNINPEEIEAKLLSGFNAVKEVAVCLHNGVLHAIILPDF
                     EKIAESGKRPSEEFFRHEVIGEYNHRVASTKKIMKFTLVQDELPKTRLGKIRRFQLPE
                     LLALIAEAESKAN"
     gene            complement(2162..3469)
                     /gene="glgC"
                     /locus_tag="P5201_04845"
     CDS             complement(2162..3469)
                     /gene="glgC"
                     /locus_tag="P5201_04845"
                     /EC_number="2.7.7.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005659275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008878 - glucose-1-phosphate
                     adenylyltransferase activity [Evidence IEA];
                     GO_process: GO:0005978 - glycogen biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate adenylyltransferase"
                     /protein_id="HRV97894.1"
                     /translation="MQTGKYGRTMGMVLAGGKGERLMPLTKYRAKPAVHFAAKYRIVD
                     FALSNLVNSGIFAIYVLVQFKSQSLNEHIERGWQFGGALRGRDFFITLVPAQMWRGEH
                     WFQGTADAIYQNLHLITLYDADRICIFAADHIYKMDVDQMLEYHMNKQADVTVAANVV
                     PSSEASAFGCIHVDDSGRIIDFIEKPANPPEIPGKPGFSYVSMGNYIFEREILEESLI
                     EDAHLPTSHDFGRDIIPKLVPKARIFAYDFSTNVLPGKTRSFWREDKPYWRDVGTIKA
                     YWEAHMDLLDHDSEMTFYNPQWPIRTVSYADPPSYVCPVKGFSCYVQSTLSAEGSRVL
                     GSRVESSVLSRNCVIMPGSDIEQSIIGEGVVVGENCRLKKVIIDSHNVIPPNTEIGFD
                     PEHDRERYAVDPNSGIITIGMPKMQLRKSIEQPKEGFDWSSFS"
     gene            complement(3542..>3756)
                     /locus_tag="P5201_04850"
     CDS             complement(3542..>3756)
                     /locus_tag="P5201_04850"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="glycogen synthase"
                     /protein_id="HRV97895.1"
                     /translation="VFEYGSPEGNGFLFSEYSAEGLLSAVGRSLDVFRNDAAGYRSEQ
                     KKAMRSDFSWDRSAPLYIDLYRSLLS"
BASE COUNT          839 a          967 c         1054 g          896 t
ORIGIN      
        1 ccgtcggcgg aggctccctt ggtgacgagg atcgtggtgc acccccgact gcgggagcag
       61 cgagcaacag aaaaaggagg aacagtacaa gatttctcgc gtgtttgttc atggtatcgc
      121 cctctttcaa aaagattgac tgcgtctatg cgaccaaaca aaagaacatc gccgttctcc
      181 tgcattgttt gactggcgga aaagaatgcg agatgcgaag ggtatcttct tcgtagtata
      241 cggctgtgcg gaagttgctt caagaggagg aaatcaggga ttcgtttgtt ttggctgact
      301 cttgcgtttt ttcgagttcg gagctgatca gcgtcttttc acaaaggcta tgggatataa
      361 ttaaaaatac tctttgacga atcccaaggg gggcttgtgc tcttatgacc gaactattac
      421 cctggacgaa gacgacgagt tttctcgcgg ctcttagtct tcgctgcact gtacgatgat
      481 atgcctgaaa cgcagtgacg cactcgcgct tatttcaaaa gagcgctcgg taacctacgc
      541 cgggctttgc gatatgatct cccggtactc cgcgctcata tcgagcacgc cgtgcacgaa
      601 ggtcgctatt ttctcggaga accggccgga gtgggtgtat atcatgtatg ccggctggaa
      661 gagcggttat accgtggtgc cgatagacta catgtccccg gccgatgagg tggcgtatat
      721 cctcgccgac tgtaaacccg agaaaatttt ttgttcccgc aggaaatttc ctgaactcga
      781 aaaagcgctt ctctccctct cctacgctcc agaaattttg gtatttgaag aatgtcttct
      841 tcctccggga gcgaatgcct ccggagatca tttcctcgaa cgcgacgacg atgatactgc
      901 tctgataatt tatacatcgg gcaccaccgg aagcccgaag ggcgccatga tctccttcgc
      961 caatattgcg gcgaacgtca gggctgtgag cgaagccgct ccgattttca cccctgaatc
     1021 gcgggttctg gtgttgctgc ccatccatca cgtgcttcct ctgggaggga caatcctcgg
     1081 acctctcttc gtgggagcgt gctgcgtctt ttcgccttcg ataggttcca gggatatact
     1141 ggagacgctc cagcacaacg gagtcacagt ggtcgtcgct gttccgcgtt tctacagcat
     1201 gctcataaag ggcatccggg agaagataca ggaaagtatt gccgcgcgga cttttttcgc
     1261 tctggcaaaa aaaattgatc atccggggct ctccaagttt ctgttcaaaa aggtccatga
     1321 gcgttttgga ggaaaaatcc gttttctggt ctgcggcggc gcggcgatcg acgacgatgt
     1381 tgcaagggac tttaaagcgc ttggattcga catgcttacc ggctacggga tgacggagac
     1441 tgcgccgctc atttcgttca cgcatccggg aaccttgagg atcggggcga gcgggtacgt
     1501 ctgtcccgcc gtcgaagtgc gtatcgtcga cggtgaaatc accgtcaggg gacgtcatgt
     1561 aatgaagggg tactacaacc gccctgaaga aacagccgcc atgatccgcg acggctggct
     1621 gcacacagga gatcttggat tcgtggatga ggatggcttt cttttcgtta ccggccgaaa
     1681 gaaggatatt attgtgcttt ccaacgggaa gaatatcaat cccgaagaga tcgaggcgaa
     1741 gctgctctcc ggatttaatg cggtgaagga ggttgcggtc tgtctccata acggggtgct
     1801 gcacgccatc attctccctg attttgaaaa aatcgcagaa agcggcaagc ggccgtccga
     1861 ggagttcttt cgtcacgagg tgatcggcga gtacaaccac cgggttgcgt cgacgaagaa
     1921 gataatgaag tttaccctgg tacaggatga actgccgaaa acacgcctcg gaaagatcag
     1981 gaggtttcag ctgccggagc tgctcgccct gattgcagag gcggagagca aggcgaactg
     2041 ataccggcgc accggaataa tttccggctt cagcgttccc ggagaggaag ggtgatcctg
     2101 tatttgccaa atatcgggaa gaacaggggg gcgtttgaac gcccccctgt tctgtctgct
     2161 gttaactgaa gcttgaccag tcgaagcctt ccttcggctg ttcgatggac ttgcgaagct
     2221 gcatcttcgg catgccgatg gtgatgatgc ctgaattcgg gtccacggcg taccgttctc
     2281 tgtcatgctc cggatcgaat cctatctcgg tgttgggcgg gatcacgttg tggctgtcga
     2341 tgatgacctt tttaagacgg cagttttccc cgacgaccac tccctcgccg atgatcgatt
     2401 gttcaatatc cgagcccggc ataatcacgc agttgcggga cagcacggac gactcgactc
     2461 tggatccaag gacgcggctt ccttccgccg aaagggtgct ttgaacgtag caggagaatc
     2521 ctttcaccgg gcagacgtac gacggagggt cggcgtagga gacggtccga ataggccact
     2581 gcgggttata gaatgtcatc tcggagtcat ggtcgagcag atccatgtgc gcctcccagt
     2641 atgccttgat ggtcccaacg tcgcgccagt agggtttgtc ctctctccag aaggagcgcg
     2701 tctttccggg aaggacgttc gtcgaaaagt cgtaggcaaa aattcgggct ttcgggacga
     2761 gttttggaat gatatccctc ccgaaatcat ggcttgtggg gagatgagcg tcctcaatga
     2821 gggactcttc aaggatttcc cgctcgaaga tatagttgcc catggagacg tatgaaaacc
     2881 ccggcttacc cgggatctcc ggcggatttg ccggtttttc gatgaagtcg ataatgcgtc
     2941 ctgaatcgtc gacgtgaatg catccgaagg cggaagcctc cgacgacggt acgacgttcg
     3001 cagccacggt aacgtcggcc tgtttgttca tgtgatattc gagcatctgg tccacatcca
     3061 tcttgtagat atggtcggcg gcaaagatgc aaattctgtc cgcatcgtag agcgtaataa
     3121 gatggaggtt ttgatagatg gcgtccgcag taccctggaa ccaatgctcg ccgcgccaca
     3181 tctgcgccgg gacgagcgta atgaaaaagt cgcgtccgcg aagggcgccg ccgaactgcc
     3241 atcctctttc gatgtgttcg ttcagggatt ggcttttgaa ctgcaccagt acataaatag
     3301 caaaaatgcc gctgtttacc agattggaaa gcgcaaaatc aacgatacgg tactttgctg
     3361 caaaatggac tgcgggtttt gcccggtatt tcgtcagcgg cataagccgc tcccctttcc
     3421 ctccagcaag caccattccc attgtacggc cgtattttcc tgtctgcatg ctgtttcccc
     3481 cctccttctc aagatggcgt tgacgaatgt cgtactgcgt tttcccgtct gtagttcacg
     3541 ctcaggacag aagtgaacga tagagatcga tgtagagcgg cgccgaacga tcccaggaaa
     3601 agtcggaccg catcgctttt ttctgttcgg aacgatatcc tgccgcgtcg ttcctaaaaa
     3661 cgtcgagcga cctccccacg gcggagagaa gaccttccgc cgagtactcg gagaaaagaa
     3721 agccgttgcc ctcgggagag ccgtactcga agaccg
//
LOCUS       DAOTIL010000177         3738 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminobacteriaceae bacterium isolate AMR_MDS_3026
            NODE_29149_length_3738_cov_4.356503, whole genome shotgun sequence.
ACCESSION   DAOTIL010000177 DAOTIL010000000
VERSION     DAOTIL010000177.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868050
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminobacteriaceae bacterium (wastewater metagenome)
  ORGANISM  Aminobacteriaceae bacterium
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae.
REFERENCE   1  (bases 1 to 3738)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3738)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:19:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,224
            CDSs (total)                      :: 3,198
            Genes (coding)                    :: 3,184
            CDSs (with protein)               :: 3,184
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3738
                     /organism="Aminobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_29149_length_3738_cov_4.356503"
                     /isolate="AMR_MDS_3026"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:3034609"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..286
                     /locus_tag="P5201_04855"
     CDS             <1..286
                     /locus_tag="P5201_04855"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HRV97896.1"
                     /translation="CSRSSLDSVVIPVHLRGQFWGFLALCFRERRSAFEGDELGVLRS
                     FACTLAASVSRWESELEVSAGYENLQKTFSDVIRTMGQIVGKKDPYTIEH"
     gene            302..790
                     /locus_tag="P5201_04860"
     CDS             302..790
                     /locus_tag="P5201_04860"
                     /EC_number="3.1.4.-"
                     /inference="COORDINATES: protein motif:HMM:NF014069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HD-GYP domain-containing protein"
                     /protein_id="HRV97897.1"
                     /translation="MASAIGARMNLDAERCEGLRIAGLVHDVGKIEIAGEILNKPGRL
                     SPIEFELVKTHSQSGYEILSGIDFPWPVAEIALQHHERLNGSGYPRGLKGNEILLEAR
                     IIAVADVVESMISHRPYRPALGMDAAREELKARKGEFYDPEVVDACLGLLEERPDIIG
                     AE"
     gene            complement(798..2093)
                     /locus_tag="P5201_04865"
     CDS             complement(798..2093)
                     /locus_tag="P5201_04865"
                     /inference="COORDINATES: protein motif:HMM:NF019310.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HRV97898.1"
                     /translation="MRHASPSSRDEARGISSRRAHFLFLCLVGAYFFSFFFRISASVV
                     LPSQAAKLGMSAALTGFISSLYYYAYSVMQSISGALHDRFGPLRVVAGGMLVAAAGTF
                     LLVLHPSEWTLGAWRLLTGFGLAPMYGGALVYQASAFTPDKYVFYSSITISVGAFGAL
                     ASVAPLGLALDTFGLSATFAGLTLLSISMSVLLWKERSFDPVVHRTPFTEQKTQSILS
                     RLAEALRLIRSSAYLRSLLVLWTVSAGALLSFQGLWAVSWYQTAYGVAAATARNWASL
                     VSVGMFIGTAVFGGFCRRPEDRFRGVCFWTLFNVASWGALVSSVALKLPLPVAGTCGF
                     LIGAANGLIAVQASSAAKEQVETAKTGALLGTMNMIVIFVVVLFQWGTGILINLFPGG
                     QAGEFTERGFLVCFLIVVVLLAGSLPALPPLRRADTRRR"
     gene            2402..3097
                     /locus_tag="P5201_04870"
     CDS             2402..3097
                     /locus_tag="P5201_04870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006301968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent transcriptional regulator"
                     /protein_id="HRV97899.1"
                     /translation="MSVSSRIEDYMETIFAIEISGREATVTDLANTLGVAKATVVAAV
                     RRLVAASLATHERYGTLALTDEGRRRALDIYRRHEHLTFLFHDILGFDPDRAMSIACA
                     MEHELDEKSEGRILGFVDYLVKARREGRSWVKDLLTAMGDERRLPRPLSMLPDGGRGV
                     VTRVTAGGLLRQRLLEMGFVPGTEVCHCSTAPLGDPLVIHVRGCEVALRKTEAVSVWI
                     CPVTGEDSAGASC"
     gene            3120..3344
                     /locus_tag="P5201_04875"
     CDS             3120..3344
                     /locus_tag="P5201_04875"
                     /inference="COORDINATES: protein motif:HMM:NF015956.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferrous iron transport protein A"
                     /protein_id="HRV97900.1"
                     /translation="MCPITIMSEGTEVTVARVAGGGESSRRLSEMGLIPGAKVRIVQN
                     GGGPLLLKVGDSRFAIGQGMAHKVFVDGSC"
     gene            3427..3735
                     /locus_tag="P5201_04880"
     CDS             3427..3735
                     /locus_tag="P5201_04880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012868865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046914 - transition metal ion binding
                     [Evidence IEA];
                     GO_process: GO:0006811 - monoatomic ion transport
                     [Evidence IEA];
                     GO_process: GO:0055072 - iron ion homeostasis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FeoA family protein"
                     /protein_id="HRV97901.1"
                     /translation="MGLTCSCSGGNERVLSALEIGEGGVVSKIAGDGSLKRRILEMGL
                     VPGTEIRVERKAPFNDPVSVLFRGYELSLRVDEANAVLVRPAGCAGCSGRWPCSAPSS
                     "
BASE COUNT          824 a          947 c         1230 g          737 t
ORIGIN      
        1 ctgctcgcgt tcttcgctcg acagcgtggt gatccctgtg cacctgcgcg gtcagttctg
       61 ggggttcctc gcgctatgct tccgggaacg gcgttccgct ttcgaaggtg atgaactcgg
      121 tgtgctgcgg agttttgcgt gcaccctggc cgcctcggtg agccgctggg agtccgaatt
      181 ggaggtgagc gccgggtacg agaacctgca gaagacgttt tcggatgtca tcaggacgat
      241 gggacagatt gttggaaaaa aagacccgta cacaatagag cactaggagc gcgtcgggac
      301 gctggcttcg gcgatcggcg ccaggatgaa cctcgatgcg gagcgctgcg agggtcttcg
      361 tattgcaggt ctcgtgcatg acgtcggcaa gattgagata gccggtgaga tattgaacaa
      421 gcccgggcgt ctttcaccta ttgagttcga gcttgtgaag acgcattccc aatcgggata
      481 tgaaatcctg agcggaatcg acttcccctg gcctgtagcc gagatcgcgc tgcaacatca
      541 tgaacggctg aacggctcgg gctatccccg gggactcaag gggaacgaga tccttctgga
      601 agcccgtatt atcgcggtcg cggatgtcgt ggagtcgatg atttcgcacc gcccctaccg
      661 tcccgcgctg gggatggatg cggcgcggga ggaactcaag gcgcgaaagg gggagttcta
      721 cgaccccgaa gttgtcgacg cgtgtcttgg tcttcttgaa gagcggccgg atatcatcgg
      781 cgcagagtag aggcgctcta tcggcgtcgt gtgtcggctc gccggagcgg cgggagcgcg
      841 ggcagactgc cggccagcag cactacaaca atcaggaagc agacgaggaa acctcgttcg
      901 gtaaactccc ccgcttgccc tccgggaaag agattgatga gtattcccgt tccccactgg
      961 aagaggacca caacaaagat gacgatcatg ttcatcgttc caagaagagc tcccgttttt
     1021 gccgtttcca cctgttcttt cgccgccgac gaggcctgga cggcaatgag cccgtttgct
     1081 gcgcctatga gaaatccgca tgtcccggcg acgggaagcg gcagtttcag cgctacggaa
     1141 ctcactaacg caccccagga cgccacgttg aagagagtcc agaaacacac cccccgaaaa
     1201 cgatcctccg gacgccggca aaacccgccg aaaacggcgg ttccaatgaa catgcccacg
     1261 ctgacaagag aggcccagtt gcgcgcggtt gcagccgcga ctccgtaggc ggtttgatac
     1321 cacgagaccg cccagagtcc ctgaaaagag aggagcgctc ccgccgagac ggtccagaga
     1381 acgagcaacg acctgaggta ggcggagctc ctgatgagcc ggagcgcttc ggccaggcgg
     1441 gagagaatcg actgcgtctt ttgctccgtg aagggcgtac ggtgcacgac aggatcgaag
     1501 ctgcgctcct tccagagaag cacgctcatg gaaatgctca aaagtgtgag ccctgcgaag
     1561 gtcgccgaga gaccgaatgt gtccagagcg agtccgagag gggcgacgga ggcgagggcg
     1621 ccgaatgcgc cgacggagat ggtgatgctt gagtagaaga cgtacttgtc cggggtgaac
     1681 gcgcttgcct gataaacgag cgcgccgccg tacatggggg ccagcccgaa accggtaagc
     1741 agacgccatg cccccagcgt ccattccgaa gggtggagca caaggagaaa cgtgcccgcc
     1801 gcggccacca gcatcccccc ggcgacgacg cggagcggcc cgaaacggtc gtgcagggcg
     1861 ccggagatcg actgcataac ggagtaggcg tagtagtaaa ggctggaaat aaagccggtg
     1921 agagcggcgc tcatgccgag ttttgccgct tgcgagggaa ggaccaccga tgcggagatc
     1981 cggaagaaaa atgaaaagaa atacgccccg acaaggcaga gaaataaaaa atgagcgcgg
     2041 cgcgaggata tgccgcgggc ttcgtcccgg gatgatggtg aggcgtggcg cataaaaatg
     2101 agctccttcc tttgccgctc ctttatcgcc gctgagaagc gtcgtgtgaa cttggcggtc
     2161 ggcaagcgtt tcaagcggtt gttctgtatc acggtgcatc ttacctcata aacggggaag
     2221 agggtaggta tgaccgccgt ttttaagagg ggctgaagaa gccggagagt gcgtttcgaa
     2281 aaataaaggt tgactgtcct tgaatcgtag cgcttcacgc ttgacaggaa ttgaagcgcg
     2341 aagtaagata agtctaacca aaaagtaagt gtgctctcac ttctcgaaag gagggaatgc
     2401 cgtgtcggtc tcatcccgaa ttgaggacta tatggagaca atcttcgcca tagagatttc
     2461 cggacgggaa gctacggtta ccgacctcgc gaatacgctc ggcgtggcca aagcaacagt
     2521 tgttgcggcg gttcgcaggc ttgtggcggc gtctcttgcg acgcacgaac gatacggtac
     2581 tctcgcgttg acggatgagg gcaggcggcg agcgctcgat atctataggc gccacgagca
     2641 tcttaccttc ctttttcacg atatccttgg cttcgacccg gaccgtgcga tgtccatagc
     2701 atgtgcaatg gaacatgaat tggacgaaaa gtcggaggga cggattctcg gatttgtcga
     2761 ttaccttgta aaggcccgca gagaggggcg ttcctgggta aaggaccttc tcacagcgat
     2821 gggagatgag cgaaggcttc caaggccgct ttcaatgctt cccgacggag gacggggtgt
     2881 tgtgactcgc gtgactgcgg gaggtttgct tcgccaacgc cttttggaaa tggggtttgt
     2941 tcccggaacg gaagtgtgtc actgcagtac ggcgccgctt ggcgatccgc ttgtgataca
     3001 cgtacgcgga tgcgaagtgg cgttgcgaaa gacggaggct gtctctgtat ggatctgtcc
     3061 tgtaactgga gaggattcag cgggagcttc ctgctgaacg aagagaggag atgggcagta
     3121 tgtgtccgat aacgatcatg tccgagggaa ctgaagtaac cgtagctcga gtggcgggcg
     3181 gcggagagtc ttcgcggcgc ctttccgaga tggggcttat ccccggagcg aaagtgcgga
     3241 tagtccaaaa cggaggaggg cctcttcttt taaaggtggg cgattcgcgt tttgcgatcg
     3301 gtcaggggat ggcgcacaag gtctttgtgg acggaagctg ttaggcggag cgctcaagga
     3361 ccggcaggtc cttttttcag attccgcgtt gagttagagt tagtgaacaa agaacggagg
     3421 gtcattatgg ggttgacgtg tagctgttcc gggggaaatg agcgtgtgtt gagcgctctt
     3481 gagataggag agggcggcgt cgtatccaaa atcgccggag acgggagctt gaagcggagg
     3541 attcttgaaa tggggctggt tccggggacg gagatacgcg tcgaaaggaa agcgcctttt
     3601 aacgatcctg tctccgtgtt gttcagaggc tatgaactga gtctcagagt cgacgaggcg
     3661 aatgctgtcc ttgtgcgccc cgcaggctgc gcgggctgtt cggggcggtg gccatgttcg
     3721 gcaccgtcat catgatcg
//
LOCUS       DAOTIL010000178         3737 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminobacteriaceae bacterium isolate AMR_MDS_3026
            NODE_29162_length_3737_cov_3.462792, whole genome shotgun sequence.
ACCESSION   DAOTIL010000178 DAOTIL010000000
VERSION     DAOTIL010000178.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868050
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminobacteriaceae bacterium (wastewater metagenome)
  ORGANISM  Aminobacteriaceae bacterium
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae.
REFERENCE   1  (bases 1 to 3737)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3737)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:19:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,224
            CDSs (total)                      :: 3,198
            Genes (coding)                    :: 3,184
            CDSs (with protein)               :: 3,184
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3737
                     /organism="Aminobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_29162_length_3737_cov_3.462792"
                     /isolate="AMR_MDS_3026"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:3034609"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..509
                     /gene="dctP"
                     /locus_tag="P5201_04885"
     CDS             <1..509
                     /gene="dctP"
                     /locus_tag="P5201_04885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014806961.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="TRAP transporter substrate-binding protein DctP"
                     /protein_id="HRV97902.1"
                     /translation="VKTPEDLKGVRIRPPGAPSWSRSVAALGATPVTMGWNDVYNAVQ
                     QKAIDGAEAQHSASYGLRVYEVLKYIDKTAHFQLLNGMIVGEKWFKTLPASYQALLIE
                     EFEKQGKLTAAEVIEKSDVFEKMMVEKGMEVVEVDLEAFKKASDAAYDELGFAELRKK
                     IYAEIGKE"
     gene            626..1135
                     /locus_tag="P5201_04890"
     CDS             626..1135
                     /locus_tag="P5201_04890"
                     /inference="COORDINATES: protein motif:HMM:NF016200.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter small permease subunit"
                     /protein_id="HRV97903.1"
                     /translation="MLDLVKKSYTFICRAEEFLTSFALCAITFLVFVSAIGRFVGYPI
                     NWAIDISLLLFAWGAFLGADIGIRKDRVINVDFLTSKMPLPVQKKIAVAWSVAIILFL
                     VVLIAYGIPLCISNAKRQFQNISLSYSYVTASLPVCAFLMIVSTAIKLKKQITDFPST
                     LRGGGADVV"
     gene            1155..2435
                     /locus_tag="P5201_04895"
     CDS             1155..2435
                     /locus_tag="P5201_04895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014806959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0031317 - tripartite ATP-independent
                     periplasmic transporter complex [Evidence IEA];
                     GO_function: GO:0015556 - C4-dicarboxylate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015740 - C4-dicarboxylate transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter large permease subunit"
                     /protein_id="HRV97904.1"
                     /translation="MLLLVGVFLALLFLGMPVAFAIGLSGFVFMLATPNIPLSIVVQR
                     IVSQTQSFTLLAIPLFIFAGNLMNATGITTRLVKLSRVLVGHMAGSLAQVSVIMSTLM
                     GGVSGSANADAVMECRILGPEMIKQGYSRGYGAAVNGLTAMITCTIPPSMGFIIYGSV
                     GEVSIGRLFVGGIVPGFLMMAMMMVTVNFTAKRRGYLPTTDRKPTAGEVLSAVRENIY
                     ALIFPVILIAGIRFGLFTPSEAGAFAAGYALFVGVFIYKEITWKVFIDTVNQSAVDIG
                     VLMLILTLSGTFGYAIVYGRVPQSIAEFLIGITSNQYLLMSLIIVMLILAGMFVETTV
                     CALLLTPVFIPVITRLGIDPVHFGVIMMTTLTSGIMTPPVGIALYSTSEIMGCTPQET
                     VKEAIPFYITLFALIVVLVLFPQIVLFLPNRVFG"
     gene            2505..3590
                     /locus_tag="P5201_04900"
     CDS             2505..3590
                     /locus_tag="P5201_04900"
                     /EC_number="4.2.1.8"
                     /inference="COORDINATES: protein motif:HMM:NF015727.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008927 - mannonate dehydratase activity
                     [Evidence IEA];
                     GO_process: GO:0006064 - glucuronate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannonate dehydratase"
                     /protein_id="HRV97905.1"
                     /translation="MKLAMVFYRHHLTKENFRYAKQLGCTHLVIHLVDYFNREKDPVT
                     GDNQPVGDSDGWGVTRNREVWSYDELRAIRNMVDEEGLVLEALENFDPGFWYDVLLDG
                     PKKIAQMDGLKQLIRNVGQAGIPIMGYNFSLAGVAARISGPFARGGAESVGCDGCDTL
                     PLPEGMVWNMVYDPEAPKGVNAATQITSEELWSRFEWFLKELLPVAEESNVKMAAHPD
                     DPPFAVVRGTPRLVWRPELYRRLLDLYPSHCNTLELCVGTLAEMNGVNYYRAIDEFAA
                     RDAIGYVHLRNVRGQVPNYRETFIDDGDIDIYRVLKILHANDFRGAIIPDHSPLVSGP
                     APWHTGMAYALGYIRAMMQRVEKDTCR"
BASE COUNT          693 a         1204 c         1125 g          715 t
ORIGIN      
        1 ccgtaaagac ccccgaggac ctcaagggcg tccgtattcg cccccccggc gcgccctcct
       61 ggtcgcgttc tgtcgccgcg ctcggcgcca ctcccgtcac catggggtgg aacgacgtct
      121 acaacgcggt gcagcagaag gccatcgacg gcgccgaagc gcagcattcg gcgagctacg
      181 gcctccgcgt gtacgaagtg ctcaagtaca tcgacaagac ggcgcacttc cagctcctca
      241 acggtatgat cgtcggcgaa aagtggttca agacgcttcc ggcgtcgtac caggcgctgc
      301 tgatcgagga gttcgagaag cagggaaagc tcaccgcggc ggaagttatc gagaagagcg
      361 acgtctttga gaagatgatg gtggagaagg gtatggaagt cgtcgaagtc gacctggaag
      421 ccttcaaaaa agcctccgac gcggcgtacg acgagcttgg cttcgcggaa ctgcgtaaaa
      481 agatttacgc ggagataggc aaggaataag ccgtcctccg aacgccccgt tccgcacggg
      541 cggcttttcg gcaaccgtcg cgtttccgga tgcggcggtt gccttctact tcgggcccgc
      601 catcgaaagg agggtgcgca agaacatgct cgatctcgtg aaaaagagct acacattcat
      661 ctgcagagca gaggagttcc tgacctcttt cgctctgtgc gcgattacat ttttggtctt
      721 cgtctccgcc atcggcaggt ttgtcgggta tccgatcaac tgggcgatcg atatttccct
      781 cctcctcttc gcatggggag cgtttctggg cgcggacatc ggcatccgaa aggaccgcgt
      841 gatcaacgtc gacttcctca catcgaagat gccccttccc gtgcagaaaa aaatcgccgt
      901 cgcctggtcg gtcgccatca tactcttcct cgtcgtgctg atcgcctacg gcattccgct
      961 ctgcatctcg aacgcgaagc ggcagttcca gaacatctcg ctcagctact cgtacgtcac
     1021 ggcgtcgctg ccggtgtgcg cgttcctgat gatcgtctcg acggcgatca aactgaagaa
     1081 acagatcacg gacttcccgt ccacgctgcg cggcggcggg gcggacgtcg tgtagcgcga
     1141 agggaggaag tccgatgctg cttttggtag gcgtctttct ggcgctgctc tttctgggta
     1201 tgccggtggc cttcgccatc ggtctgtcag ggttcgtctt catgctggcg acccccaaca
     1261 tccccctctc catcgtcgtc cagcgcatcg tctcgcagac gcagagcttt acgctcctgg
     1321 ccattccgct cttcatcttc gcgggcaacc tgatgaacgc caccggcatc acgacgcgcc
     1381 tggtcaagct ctcccgcgta ctcgtgggac acatggcggg cagcctcgcc caggtcagcg
     1441 tcatcatgag cacgctgatg ggcggcgtct ccggctcggc gaacgccgac gcggtgatgg
     1501 agtgccgcat cctgggcccc gagatgatca agcaggggta ctcgcgcggc tacggcgcgg
     1561 cggtcaacgg tctcaccgcg atgatcacct gcaccatccc tccgagcatg ggcttcatca
     1621 tctacggctc ggtgggggag gtctccatcg gcaggctctt cgtcggcggc atcgtcccgg
     1681 gattcctgat gatggccatg atgatggtca ccgtcaactt cacggcgaag cgccgcggct
     1741 atctgcccac caccgacaga aaaccgaccg cgggcgaggt gctgtcggct gtgagggaga
     1801 acatctatgc tctgatcttc ccggtcatcc tgatcgcagg aatccgcttc ggcctgttca
     1861 cgccctccga ggcgggagcg ttcgccgcgg ggtacgcgct cttcgtcggc gtcttcatct
     1921 acaaggagat cacatggaag gtcttcatcg acacggtgaa ccagagcgcg gtggacatcg
     1981 gcgtgctgat gctcatcctc acgctctccg gcacgttcgg ctacgcgatc gtctacgggc
     2041 gcgtgccgca gtcgatcgcc gagttcctga tcgggatcac ctccaaccag tacctgctga
     2101 tgtccctgat catcgtcatg ctgattctgg cggggatgtt cgtcgagacg accgtgtgcg
     2161 cgctgctgct gacccctgtc ttcatcccgg tgatcacgcg gctgggcatc gacccggtgc
     2221 acttcggcgt cattatgatg acgacgctga ccagcgggat catgacgccg ccggtgggca
     2281 tcgccctcta ctcgacgtcg gagatcatgg ggtgcacgcc ccaggagacg gtgaaagagg
     2341 cgatcccctt ctacatcacg ctcttcgccc tcatcgtcgt cctggtgctc tttccgcaga
     2401 tcgtcctctt cctgcccaac cgggttttcg ggtaatatac gcgggaggct ttcaataacg
     2461 acgttgccac aggctcttca gagccgcgaa gggagaagtg acgcatgaaa ctcgcaatgg
     2521 tcttctaccg tcatcatctg acgaaggaga acttccgtta cgcaaagcag ctcggctgca
     2581 cccacctggt gatccatctg gtggactact tcaacagaga aaaggacccc gtcacgggag
     2641 acaatcagcc cgtcggggac tccgacggct ggggcgtgac ccgcaaccgc gaagtctgga
     2701 gctacgacga gctgcgcgcc atccggaaca tggtggacga ggaggggctc gtcctggaag
     2761 ccctcgagaa cttcgacccc ggcttctggt acgacgtcct gctcgacggc ccgaagaaga
     2821 tcgcgcagat ggacggcctc aaacagctta tccgcaacgt cggacaggcg ggcatcccga
     2881 tcatggggta caacttcagc ctcgcagggg tggcggcgcg catcagcggc ccgttcgccc
     2941 gcgggggggc ggagtcggtc ggctgcgacg ggtgcgatac gctgccgctg ccggagggaa
     3001 tggtgtggaa catggtctac gacccggagg ctccgaaggg tgtcaacgcg gcgacgcaga
     3061 tcacctcgga agagctgtgg agccgcttcg agtggttcct gaaggagctg cttccggtgg
     3121 cggaggagtc caacgtgaag atggcggcgc accccgacga tccccccttc gccgtcgtcc
     3181 ggggaacgcc gcgcctcgtc tggaggcccg aactctaccg ccgcctcctg gacctgtacc
     3241 cgagccactg caacacgctg gagctgtgcg tcggcacgct cgccgagatg aacggcgtca
     3301 actactaccg cgccatcgac gagttcgccg cgcgggacgc catcggctac gtccacctgc
     3361 gcaacgtccg cggacaggtg cccaactaca gggagacctt catcgacgac ggcgacatcg
     3421 acatctacag ggtgctcaag atactgcacg ccaacgactt ccggggcgcg atcatccccg
     3481 accactcccc gctggtcagc ggcccggccc cgtggcacac cggcatggcc tacgccctcg
     3541 gctacatccg cgccatgatg cagcgcgtgg agaaggacac ctgccggtaa aaaagacccg
     3601 gaggaggagg ggatgcgttc ccttctcctc ccgcccgaat tttttcgact ctcccgaaaa
     3661 cccacgcagc tccctgacgc tcgcttcccg gcgatatgga gcgctcgccc cgaactgaag
     3721 aattttcctc tctcctt
//
LOCUS       DAOTIL010000179         3693 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminobacteriaceae bacterium isolate AMR_MDS_3026
            NODE_29613_length_3693_cov_3.990929, whole genome shotgun sequence.
ACCESSION   DAOTIL010000179 DAOTIL010000000
VERSION     DAOTIL010000179.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868050
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminobacteriaceae bacterium (wastewater metagenome)
  ORGANISM  Aminobacteriaceae bacterium
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae.
REFERENCE   1  (bases 1 to 3693)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3693)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:19:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,224
            CDSs (total)                      :: 3,198
            Genes (coding)                    :: 3,184
            CDSs (with protein)               :: 3,184
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3693
                     /organism="Aminobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_29613_length_3693_cov_3.990929"
                     /isolate="AMR_MDS_3026"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:3034609"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..346
                     /locus_tag="P5201_04905"
     CDS             <1..346
                     /locus_tag="P5201_04905"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRV97906.1"
                     /translation="LTLLVGVLNILSGGLTLAKALPSLLKTRKMLPSVPVSSLLLRLY
                     STQIALGGLSVMFGSSMLAPGIIPGLLIGVILAANGGVLLYLMRLLLLLDALAESAGG
                     TQPESDEQSVCS"
     gene            381..1814
                     /locus_tag="P5201_04910"
     CDS             381..1814
                     /locus_tag="P5201_04910"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4139 domain-containing protein"
                     /protein_id="HRV97907.1"
                     /translation="MKSIQILCTFLFLLVFLSALPALGAEVVSSTEADQTGVEVTVYN
                     TDLALVKDVRSILLKEGQGELRFMDVASSIRPETVHVKSLNNPPSLAILEQNYEYDLI
                     SPEKLLEKFVGRTVKIEVWNDYQGKKEIVEGTLLSTNNGLVFDIGGQIYLNYPGLKIV
                     PSIPDNLYARPTLAWLYANSAKEKHSVEVSYLTGGMTWKADYIFVLDDADTAADLTGW
                     VTLNNRSGAAYRDATLKLIAGEVQTVADVLPPPMMRMAMGKGVPEAAPAFEEEAFFEY
                     HIYNLARKTTIGNNQTKQISLLEAFGTKIGKEYITRGERRYFTSSLQRGLLKQKVDVV
                     ISFKNSEENNLGMPLPKGIIRMYKKDSKGSLQFIGEDRIDHTPRNEEVRLKVGEAFDV
                     VAERRQTRYKKLADNLYETSWELIVRNRKKDESVVVSFNEPVWGDWEVIASTHPYEQT
                     DAFTLRFDVPAKPDEEVVVKFSLRIRS"
     gene            1857..2465
                     /locus_tag="P5201_04915"
     CDS             1857..2465
                     /locus_tag="P5201_04915"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRV97908.1"
                     /translation="MGEWWSGMLMFEKVFWYIAIPFTALFFIQLVLTFTGMGGEDADG
                     GMEGDAGGEDFDVEGDGDGDIGEAGGFQIFTVKNFITFFTVFGWSGITLHNAGANEYL
                     VLLGSLLIAVAVMLLVALLFVSITRLAQSGTMDVKNALGRTGEVYLPIPAGRAGHGKI
                     SITLQGALREVDAVTAGEALPRGTSVRVVDVAGNGVLLVEKA"
     gene            2497..>3693
                     /locus_tag="P5201_04920"
     CDS             2497..>3693
                     /locus_tag="P5201_04920"
                     /inference="COORDINATES: protein motif:HMM:NF013324.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH domain-containing protein"
                     /protein_id="HRV97909.1"
                     /translation="MTFGLILITLIVIIIFTTVAALFARYKKCPSDKILVVYGKVAKV
                     DGQHQSSKCIHGGAAFIWPVIQAYHFLDLTPLSIEVNLRNALSKQNIRVDVPSRFTVG
                     ISTEPGIMQNAAERLLGLQLAEIQELSKDIIFGQLRLVIATMDIEEINTDRDKFLANV
                     TANVEAELKKIGLRLINVNVTDINDESGYIQALGKEAAAKAVNDAKKSVAEQDRDGSI
                     GEANAQRDQRIQVAQANAAAVEGENLSKITIAQSEAERREKQAESNRLAEAAEKVQAA
                     KALEESYKAEEEAELARARREKATQEADVLVHAGIEKERIQIRADADAEEMRRTAAGE
                     ADAIFAKMSAEARGLREILVKQAEGFERLVKAAGGDTDKAIMMMIADKLPELVKTQVE
                     AVKGIKI"
BASE COUNT          788 a         1134 c         1161 g          610 t
ORIGIN      
        1 gctgacgctc ctcgtcggtg tgctcaacat cctgagcgga ggtcttacgt tggcgaaggc
       61 gctcccctcg ttgctgaaga cgaggaagat gctgccgtcc gttccggttt cgtcgctcct
      121 cctcaggctg tactcgacgc agatcgccct ggggggactc tcggttatgt tcggctcgtc
      181 gatgctcgcg cccggtatca tccccggcct gctcatcggc gttattctcg ccgcgaacgg
      241 cggcgtgctg ctctacctga tgcgcctgct ccttctgctc gacgccctcg cggaatccgc
      301 gggcgggacg cagccggaga gcgatgaaca gagcgtgtgt tcctaaaaat tgcgacaaac
      361 taaaaaagga ggtctgtgaa atgaaaagca ttcagatact gtgcacgttc ctttttctgc
      421 tcgtctttct ctcggccctg cctgcactgg gggcggaggt ggtctcgagc acggaagcag
      481 atcagacggg ggtcgaggtc acggtgtaca acacggacct cgccctggtg aaggacgtca
      541 ggagcatcct gctcaaagag gggcaggggg agctccgctt catggacgtg gcttcttcca
      601 tccggccgga gacggtgcat gtgaagtcgc tcaacaaccc gccttcgctg gcgattctgg
      661 agcagaacta cgagtacgac ctcatcagcc cggagaagct gctggagaag ttcgtcgggc
      721 gcacggtgaa gatcgaggtg tggaacgact accaggggaa gaaggagatc gtggagggca
      781 cgcttctgag cacgaataac ggactcgtct tcgacatcgg cggccagatc tacctgaact
      841 accccggact gaagatcgtc ccgtccatcc ctgacaacct ctatgcgcgc cccactttgg
      901 cgtggctcta cgccaactcg gcgaaggaga agcacagcgt cgaggtctcc tacctgacgg
      961 gcgggatgac gtggaaggcg gactacatct tcgtcctcga cgacgccgac acggcagccg
     1021 acctcaccgg ctgggtcacg ctgaacaaca gaagcggcgc ggcgtaccgg gacgcgacgc
     1081 tcaagctgat cgccggcgaa gtgcagacgg tcgccgacgt tcttcctccg cccatgatgc
     1141 ggatggcgat ggggaagggg gtacccgaag ccgcgcctgc cttcgaagaa gaggcttttt
     1201 tcgagtacca catctacaat ctggcgagga agacgacgat agggaacaac cagaccaagc
     1261 agatcagcct gctggaggcg ttcggcacga aaatcggcaa ggagtacatt acccgcggcg
     1321 agcggcgcta cttcacctcc tcgctccagc gggggctgct gaagcagaag gtcgacgtcg
     1381 tcatctcctt caaaaattcc gaggagaaca acctcggcat gccccttccc aaggggatca
     1441 tcaggatgta caagaaagac tccaaaggaa gccttcagtt catcggcgaa gaccgcatcg
     1501 accacacgcc gcgcaacgag gaggtccgtc tgaaggtggg cgaggccttc gacgtggtcg
     1561 cggaacgccg tcagacgcgc tacaagaagc tggccgacaa cctctacgag acgtcgtggg
     1621 agcttatcgt cagaaaccgc aaaaaagatg agtctgtcgt cgtctccttc aacgagcccg
     1681 tctggggcga ctgggaagtg atcgcatcga cccatccgta cgaacagacc gacgccttca
     1741 cgctccgctt cgacgtcccc gccaaacccg acgaagaggt cgtggtcaag ttcagcctgc
     1801 gaatccgcag ctgacgacac atgcgaaacc ctgtcggcgc aaaggaggcg gtgtgagtgg
     1861 gggagtggtg gagcggcatg ctcatgtttg aaaaagtctt ctggtatatc gcaattccct
     1921 tcaccgcgct cttcttcatc cagctcgtgc tcaccttcac gggcatgggc ggggaggacg
     1981 cggacggcgg aatggaaggc gacgcaggag gagaggactt cgacgtcgag ggtgacggcg
     2041 acggggatat cggcgaggcc ggaggtttcc agatcttcac tgtgaagaac ttcatcacct
     2101 tcttcaccgt cttcggctgg agcggcatca cgctccacaa cgccggagcg aacgaatatc
     2161 tcgtcctcct gggatcgctg ctcatcgccg tcgcggtcat gctgctcgtc gcgctgctct
     2221 tcgtctccat cacgcggctg gcgcagagcg ggacgatgga cgtcaagaac gcgctcggca
     2281 gaacgggcga agtctacctt cccatccccg ccggacgtgc cggccatgga aaaatctcca
     2341 tcacgctgca gggcgcgctc cgcgaggtcg acgccgtgac ggcgggggag gcgctcccca
     2401 ggggaacctc ggtgcgggtc gtggatgtcg ccggcaacgg cgtgctgctg gtcgagaaag
     2461 cgtaatacac ccaacatctg aaaggagagg attcgcatga cgttcggtct gattctgatc
     2521 accctgatag tcatcatcat cttcaccacc gtggcggcgc tcttcgcgcg ctacaagaag
     2581 tgcccctccg acaagatcct cgtggtctac ggaaaggtcg ccaaggtcga cgggcagcat
     2641 cagtcctcca agtgcatcca cggaggcgcg gcgttcatct ggcccgtcat ccaggcctac
     2701 cactttctcg acctgacgcc cctctccatc gaggtcaacc tcagaaacgc gctcagcaag
     2761 cagaacatcc gcgtcgacgt cccctcgcgc ttcacagtcg gcatttccac cgagccgggc
     2821 atcatgcaga acgccgccga gcgccttctg ggtctccagc tcgcggagat acaggagctg
     2881 tccaaggaca tcatcttcgg ccagctccgc ctcgtcatcg ccaccatgga catcgaggag
     2941 atcaacaccg accgcgacaa gttcctcgcc aacgtcaccg ccaacgtgga ggccgaactg
     3001 aagaagatcg gtctgcgcct gatcaacgtc aacgtcaccg acatcaacga cgaatccggc
     3061 tacatccagg cgctcggcaa ggaggcggcg gccaaggcgg tcaacgacgc gaagaagagc
     3121 gtcgccgaac aggaccgcga cggctccatc ggcgaggcca acgcgcagag ggaccagcgg
     3181 atccaggtcg cccaggcgaa cgccgccgca gtcgaggggg agaacctgtc gaagatcacc
     3241 atcgcgcagt cggaggccga gcgccgcgag aagcaggccg agtccaacag gctcgcggag
     3301 gctgcggaga aggtgcaggc ggccaaggcg ctggaggagt cgtacaaggc ggaggaggag
     3361 gccgaacttg cccgcgccag gcgcgagaag gccactcagg aggccgacgt gctggtgcac
     3421 gccggaatcg agaaggagag aatccagatt cgggccgacg ccgacgccga ggagatgcgc
     3481 cggacggccg cgggcgaggc cgacgccatc ttcgcgaaga tgagcgccga ggccagggga
     3541 ctccgcgaga tcctcgtcaa gcaggcggag ggcttcgagc ggcttgtcaa ggcagccggc
     3601 ggcgacaccg acaaggccat catgatgatg atcgccgaca aactccccga actggtgaag
     3661 acgcaggtcg aggccgtcaa ggggatcaag atc
//
LOCUS       DAOTIL010000180         3680 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminobacteriaceae bacterium isolate AMR_MDS_3026
            NODE_29763_length_3680_cov_3.742621, whole genome shotgun sequence.
ACCESSION   DAOTIL010000180 DAOTIL010000000
VERSION     DAOTIL010000180.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868050
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminobacteriaceae bacterium (wastewater metagenome)
  ORGANISM  Aminobacteriaceae bacterium
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae.
REFERENCE   1  (bases 1 to 3680)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3680)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:19:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,224
            CDSs (total)                      :: 3,198
            Genes (coding)                    :: 3,184
            CDSs (with protein)               :: 3,184
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3680
                     /organism="Aminobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_29763_length_3680_cov_3.742621"
                     /isolate="AMR_MDS_3026"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:3034609"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            137..1000
                     /locus_tag="P5201_04925"
     CDS             137..1000
                     /locus_tag="P5201_04925"
                     /inference="COORDINATES: protein motif:HMM:TIGR00196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052855 - ADP-dependent NAD(P)H-hydrate
                     dehydratase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-hydrate dehydratase"
                     /protein_id="HRV97910.1"
                     /translation="MSSFSAFLPQRPASIHKGDRGGVLIVGGSSLYRGAPVLAARGAL
                     RSGCGLAVIFSEEVVCAAAASSLPECIVQAGLFTSSRKEIINVLESWESRIECLVLGP
                     GLGRSPEAGEIVRLVLEKWEKPMILDGDGIFWLSQYASVFKRRASLLLTPHEGEAACL
                     LGASIESVRCARGESARRIAVRDGIVLLKGPQTLVDDGVRCEMIDEGNQALAIPGSGD
                     VLSGSIAAFSAAGLPLFESACLGAVVHGRAGRLVSRKLGIDGVLASEVADALPLILQK
                     MRMRERETKTN"
     gene            1732..2409
                     /gene="tsaB"
                     /locus_tag="P5201_04930"
     CDS             1732..2409
                     /gene="tsaB"
                     /locus_tag="P5201_04930"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: protein motif:HMM:TIGR03725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003725 - double-stranded RNA binding
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     dimerization subunit type 1 TsaB"
                     /protein_id="HRV97911.1"
                     /translation="MKEYTLSVDCCSAWTTLGLAEDEKVRGEINIDAGKNQSALLPGL
                     LQHFLKAFSLTPEDISLFSVVTGPGSFTGIKVGISFVTFLAWAAGKSIVPLSSLECMA
                     FEKIRRSGGLAASVLWGGGGKVYGGLFKGEGDTLPPLSLFRSGSFTPELFLEAFSGTK
                     LRHQDVFWLTDAPEKVAPLFPSFGGSFEKIIPTGSATVELTRRHKGRARSAFEIHADY
                     FRDPDLG"
     gene            2572..2784
                     /locus_tag="P5201_04935"
     CDS             2572..2784
                     /locus_tag="P5201_04935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006583470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HRV97912.1"
                     /translation="MPKKFEVLVNNSWCKGCSLCINICPKKVLELDEVRLKSVAARPE
                     DCIGCRQCENICPDLAITVKEDTKDA"
     gene            2777..3574
                     /locus_tag="P5201_04940"
                     /pseudo
     CDS             2777..3574
                     /locus_tag="P5201_04940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008520743.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoacid:acceptor oxidoreductase subunit alpha"
BASE COUNT          872 a          903 c          987 g          918 t
ORIGIN      
        1 gatccgctcc aatcatctgc tgttctcaag ctctccaagg cctcctgccg cacaaaggaa
       61 cggaaaggat ccacctctcg ctctctcatg acggagacta tgcggtcgcg atggtcgtcc
      121 tggagatagg cttccaatga gcagtttttc cgcattcctt ccccaaaggc ccgcgagtat
      181 tcataaaggc gacagaggcg gggtgcttat cgtcggaggc agttcactgt acagaggagc
      241 gccagtcctc gcggcgcggg gagcgcttcg ctccggatgc ggtctagcgg ttattttttc
      301 tgaggaagtc gtttgcgccg ccgcagcttc ttctcttcct gaatgcatag ttcaggccgg
      361 tcttttcact tcctcacgaa aagagataat caatgtcttg gaatcctggg aatcccgtat
      421 agaatgcctg gtgcttggcc cgggtcttgg aagatcaccg gaagccgggg aaattgtccg
      481 cctagtactg gaaaagtggg aaaaacctat gattcttgat ggagacggca tcttttggct
      541 ttcgcagtat gcaagtgttt ttaaacgtcg cgcttctctt ctcctgactc cgcacgaagg
      601 agaagcagcg tgccttcttg gcgcttcgat cgaatcggtc cgttgcgctc gtggtgaatc
      661 cgcccgaagg atcgccgtcc gggacggtat tgttcttttg aaaggaccgc aaacgcttgt
      721 agatgatgga gtccgctgcg aaatgattga cgagggaaac caggcgcttg cgattcctgg
      781 ttcaggagat gttctttccg gctccattgc agccttttcg gccgcagggt tgccgctctt
      841 cgagagcgca tgcctcggcg ctgtagtcca tggacgcgcc ggaaggctcg tatcacgaaa
      901 actagggatc gacggagtgt tagcttcgga agtcgccgac gctcttccgc tgatccttca
      961 gaaaatgagg atgagagaac gtgagacgaa aacgaattga ctgggatgaa gaaattagaa
     1021 aaaccgaaca agttcgctgg aaaggtcttc gcttcagcat cgcgagtttt gcgcttgccg
     1081 cgttctggat tttcggcgta ggacgaatgg gaagaaagag tctttccatg gggcctctaa
     1141 ctttggtgct tctctttgtg gccgccggga ctcttctttt catcatgttg atgcggagaa
     1201 aacagaaatg ataggggacg cttaaccgat ggattcgatt tcatgctgtg aatacatctc
     1261 tttttccgag gaaagcacca tgcaaattgg agagcttatg gcggaacact cctttccggg
     1321 acttgtattt ctcctggagg gcgatttggg aacaggaaaa acgacgcttg taagagggtt
     1381 ttgctgggca gacggataca tcggcgtgcg cagcccttca tttacactgg ttaatcgtta
     1441 tttttgcgga gagaaaaccg tagtgcactc cgacctttat cgcctgtctt cggtcgatcc
     1501 taccgaactc gatgtagaga gttatttgga tgaccggagt gttctcttcg tggaatgggc
     1561 agaccgaggg gtacccggag acttcagcga aatatggaga attcgttttc gatatgaaga
     1621 cgaagaagag gacgagggac gaagagtgct ctctttttcg gcccaaggcg cccgcgcaga
     1681 gaaagccctg gaatcttttc tgggaaaact caaggagagg aagaaaaaat aatgaaagaa
     1741 tacactcttt cagttgactg ctgctcggca tggaccacac tcgggctggc ggaagacgag
     1801 aaggtacgcg gagagatcaa catcgatgcc ggaaaaaatc agtcggctct ccttccgggg
     1861 ctccttcaac actttctgaa agcattctcc ctcacaccgg aggacatctc tcttttcagc
     1921 gttgtcaccg gccccggttc cttcaccgga atcaaggttg gaatttcttt cgtgaccttt
     1981 cttgcgtggg ctgcgggaaa aagtattgtt ccgttgtctt cgcttgaatg catggccttc
     2041 gaaaagatca gaagaagcgg cggtctggct gcttcggtac tttggggagg aggtgggaaa
     2101 gtgtatggcg gtcttttcaa aggagaagga gacacgcttc ctccgctcag tcttttccgc
     2161 agcggatcct ttactccgga gttattcctc gaagcttttt ccgggacgaa gcttcgtcat
     2221 caagatgtct tttggctcac agacgctcct gagaaagtag cccctctttt cccttctttc
     2281 ggcgggtcct tcgaaaaaat cattccaacc ggttccgcga cagtcgaatt gacgcggcgc
     2341 cacaaaggcc gtgcacgttc tgctttcgaa atacacgccg attattttag ggatccagac
     2401 ctcggataga ttttatcatt tttttcttta gaaaatcgaa gagataaaaa agaaattatt
     2461 atatttgatg ttgaactgtg ttgaggttca agctatccta ctcatcagaa gagcactaga
     2521 agatgcgcac gctcctttct gtttaggaga aattcgtagg aggtgacttt tttgccgaag
     2581 aaattcgaag ttttggtgaa taattcttgg tgtaagggat gttccttatg tattaatatc
     2641 tgtccaaaaa aagttctcga gcttgacgaa gtccgtttga aatcggtagc ggcaagacct
     2701 gaagattgca tcggatgcag acagtgcgaa aacatctgtc ccgatcttgc gatcacagtg
     2761 aaggaggata cgaaggatgc ctgatgtcgc tttctggcaa ggtaacgaag cgcttgcaca
     2821 cggagcactt gcggctggat gccgtttttt tgcaggatac cccataaccc cttccaccga
     2881 gatcgcagag atcatggcgg aggaacttcc caagcttaac ggacgcttta tccaaatgga
     2941 ggatgaaatt gccggaatcg cggcaaccat tggaggctcc attgccggta ggaaagcttt
     3001 gacagccact tccggccccg ggttctccct aaagcaggaa aatcttggtt ttgcctacat
     3061 cgcggagatt cccatcgttg tcgtcgacgt tatgagaggc ggtccttcaa ccggtttgcc
     3121 aacaagaaca gcccagcagg atgtcatgca ggctcgctgg ggaacacacg gcgatcatgg
     3181 cacaatctgt tatgccccct catccgtgca ggagtgctac gagatcgcga tcaaggcctt
     3241 caacgccgcg gagagatacc gtcagccggt tctgatcttg agcgatgaga ttattgggca
     3301 catgagggag aaagttgttc ctcccactat caaacctgag gaccttgtgg agagaaaacg
     3361 ccccaccgat attcctgaaa aattcgttcc ctaccgtcct gaccctaagg acgatcttcc
     3421 ttgtatggct tccttcggcg acggctatac atggcatatc accggcctga cccataacga
     3481 ctggggattc cccacgaata actccgatga aattgaaaaa atgatgaagc gactgatgcg
     3541 gaagatagac cgtttccgcg acgatatcgt cgagccccgc cccgatcttt gacgaggacc
     3601 gcgtcagccg gtcgcgcagc ttcgagaaaa acgacatgca cccatccttt tcgtctgtcg
     3661 cgcagaacct aaggactctc
//
LOCUS       DAOTIL010000181         3662 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminobacteriaceae bacterium isolate AMR_MDS_3026
            NODE_29955_length_3662_cov_2.964236, whole genome shotgun sequence.
ACCESSION   DAOTIL010000181 DAOTIL010000000
VERSION     DAOTIL010000181.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868050
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminobacteriaceae bacterium (wastewater metagenome)
  ORGANISM  Aminobacteriaceae bacterium
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae.
REFERENCE   1  (bases 1 to 3662)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3662)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:19:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,224
            CDSs (total)                      :: 3,198
            Genes (coding)                    :: 3,184
            CDSs (with protein)               :: 3,184
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3662
                     /organism="Aminobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_29955_length_3662_cov_2.964236"
                     /isolate="AMR_MDS_3026"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:3034609"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..434
                     /locus_tag="P5201_04945"
     CDS             <1..434
                     /locus_tag="P5201_04945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009165274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016624 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, disulfide as
                     acceptor [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent enzyme"
                     /protein_id="HRV97913.1"
                     /translation="ISNYQKHHMNISDIADRAGAYGVPGVSVDGNDVIAVYEAASEAV
                     ARARRGDGPSIVECKTWRHRGHFEGDPGKYKDPEEQKAWLAKDPIPRIEAKLIELGMA
                     TQADIDAMRAKVDEETEAAIKFAEDSPEPGDDELLTDVWAG"
     gene            467..1438
                     /locus_tag="P5201_04950"
     CDS             467..1438
                     /locus_tag="P5201_04950"
                     /EC_number="1.2.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009164905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-ketoacid dehydrogenase subunit beta"
                     /protein_id="HRV97914.1"
                     /translation="MAQMTYMEALRDGMRMEMRRDKNVFLAGEDVGFFGGCFGQTAGL
                     FDEFGKERVVDTPITETAILGLGVGSAAAGLRPIVELMFMDFMGVCMDEINNQAAKMR
                     YMFGGKAKLPMVVRTTCGAGMSAAGQHSQSLEAWLAHIPGVKVVMPATPADAKGLMEG
                     AVRDDNPVFFIEHKGLFGISGEVPDGEYVVPPGKAAVLREGKDVSLIAWSAMVHKALA
                     AADMLAKDGISAEVIDLRSIAPLDKQTVLDSVCKTGRVVIVHEACRTGGFGGEIAAII
                     ADEAFDSLNAPVKRVTAPDTPVPFNARLEQLYIPSAEKVVATVKELF"
     gene            1478..2506
                     /locus_tag="P5201_04955"
     CDS             1478..2506
                     /locus_tag="P5201_04955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009165276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003951 - NAD+ kinase activity [Evidence
                     IEA];
                     GO_process: GO:0006741 - NADP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(+)/NADH kinase"
                     /protein_id="HRV97915.1"
                     /translation="MEKDAFVGIIANPASGKDIRRLVAYGTVFDNQEKVNIVRRILLA
                     LDALGVGRVLAMPDYYGIVRRAAEGVRSDRPLRLAVEMLDMPLTGTQIDSQRAAELLC
                     TAGCGCIVTLGGDGTNRMVAKGCRDVPLMPVSTGTNNVFPLLIEGTTAGLAAGVTALG
                     CGEECTVRRSKKLTIFKNGEAADIALIDAVVLQSRFIGSRAMWEVENMRCCAVTRGEP
                     HNIGVASVVGNLSPITPEDPHGGFLKFDPQRADIFAPIAPGLFLPVGVASFCALQPGE
                     EIQVAPVPSIIALDGEREVDLHAEDSASIRLDRDGPRVVDVPAALRWAAESGFFGNKE
                     LVRRLAPL"
     gene            2623..>3662
                     /locus_tag="P5201_04960"
     CDS             2623..>3662
                     /locus_tag="P5201_04960"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012424.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoamide acetyltransferase family
                     protein"
                     /protein_id="HRV97916.1"
                     /translation="MAVEIVMPKMGLTMTVGSVGKWLKKEGDQVTKGEHVVEVLTEKI
                     TNLVDAPADGVLRIAAPEGTTLPIGGVMGYVGAAGEALPGLPQQEPQSVAATVSQSRE
                     GAAPLPPSAGGAPGGGFPAERPAHVRISPVARKMAQEHNIDYARIQGTGPDGRITKED
                     IEAVLASPAAAPQAPLPAAGFDAAPRPDGVSWAAPLPGSFEERPYSGVRRIIGDRMLE
                     SWSAAPKVTHHARADAKALLELRASINETLEKEERVSVTDMLVKITAMALRQKLFMNV
                     SLRGDRIVTFREVHLGVAVALEGSLIVPVIRNADEKSLTRISRELKDLSRRARENALA
                     PEEISGGTFTLT"
BASE COUNT          663 a         1114 c         1329 g          556 t
ORIGIN      
        1 gcatctccaa ctaccagaag caccacatga acatcagcga catcgccgac cgcgccggag
       61 cgtacggcgt gcctggggtc agcgtggacg gcaacgacgt gatcgccgtt tacgaggcgg
      121 cctccgaagc cgtggcgcgc gcacgccggg gcgacggtcc gagcatcgtc gagtgcaaga
      181 cctggcgcca tcgcggccac ttcgagggcg accccgggaa gtacaaggac ccggaggagc
      241 agaaagcctg gctcgcgaag gacccgatcc cgcgcatcga ggccaagctg atcgagctgg
      301 gcatggcgac gcaggccgat atcgacgcga tgagggcgaa ggtcgacgag gagaccgagg
      361 cggccattaa gttcgccgag gacagccccg aaccgggcga cgacgaactt ctcaccgacg
      421 tgtgggccgg ttgagcggcg cggatgaccg aaggagagtg gcgaagatgg cacaaatgac
      481 atatatggaa gcgctcaggg acggaatgag gatggagatg cgccgggaca agaacgtctt
      541 tctcgccggc gaggatgtgg gattcttcgg cgggtgtttc ggccagacgg cggggctctt
      601 cgacgagttc ggcaaggagc gcgtggtgga tacccccatc acggagacgg cgatactggg
      661 actgggggtc ggttccgcgg cggcggggct ccgtcccatc gtcgagctga tgttcatgga
      721 cttcatgggc gtctgtatgg acgagatcaa caaccaggcg gcaaagatgc gctacatgtt
      781 cggcggcaag gccaagctcc cgatggtggt ccgcaccacg tgcggcgcgg ggatgtccgc
      841 agcggggcag cactcgcagt cgcttgaggc gtggctggca catattccgg gagtcaaggt
      901 ggtcatgccc gcgactccgg ccgacgccaa ggggctgatg gagggcgccg tccgggacga
      961 caacccggtc ttcttcatcg agcacaaggg gctcttcggt atttcgggcg aggtccccga
     1021 cggcgagtac gtcgtgcctc cggggaaggc ggccgtgctc cgcgagggga aggacgtctc
     1081 gctgatcgcg tggtccgcga tggtccacaa ggcgctcgcc gccgccgaca tgctggcgaa
     1141 ggacggtatc tccgccgagg tgatcgacct gcggagcatc gcgccgctgg acaaacagac
     1201 agttctcgac tctgtctgca agacggggcg cgttgtcatc gtccacgagg cgtgccgcac
     1261 cggcggcttc ggcggcgaga tcgcggccat catcgccgac gaggcgttcg acagtctgaa
     1321 cgccccggtg aagagggtga cagcgcccga cacgccggtg ccgttcaacg cgcgtctgga
     1381 gcagctctac ataccgagcg cggagaaagt cgtcgcaacg gtcaaggaac tcttctaagc
     1441 gctccggagc ggccggggaa aggggagggg ctccatcatg gaaaaagacg cttttgtcgg
     1501 aatcatcgcc aatcccgcgt cgggaaagga catccggcgc ctggtcgcct acggcacggt
     1561 cttcgacaat caggagaagg tgaacatcgt ccggcggatt ctgctggcgc tcgacgctct
     1621 cggcgtgggg cgcgtgctcg cgatgccgga ctattacgga atcgtccgca gggcggcgga
     1681 aggggtgcgc tccgaccgcc ccctgcgcct cgccgtggag atgctcgata tgccgctcac
     1741 cggcacgcag attgactcgc agcgcgctgc ggaacttctc tgcacggccg ggtgcggctg
     1801 catcgtcacg cttggagggg acgggacgaa ccggatggtg gcgaaggggt gccgcgacgt
     1861 ccccctgatg cccgtctcca cggggacgaa caacgtcttc cccctgctca tcgaggggac
     1921 gacggcgggg ctcgccgccg gggtgacggc gctcggctgc ggcgaggagt gcacggtccg
     1981 gcggagcaaa aaactcacga ttttcaagaa cggggaggcg gcggacatcg ccctcatcga
     2041 cgcggtggtg ctgcagagcc gcttcatcgg cagccgagcg atgtgggagg tggagaacat
     2101 gcgctgctgc gcggtgacga ggggggagcc gcacaacatc ggcgtcgcct ccgtcgtggg
     2161 gaatctctcc ccgatcacgc cggaggaccc gcacggcggc ttcctgaagt tcgatccgca
     2221 acgcgcggat atcttcgccc ccatcgcgcc gggactcttt ctcccggtgg gggtcgcgtc
     2281 gttttgcgcg ctccagccgg gggaggagat tcaggtcgct cccgttccca gcatcatcgc
     2341 gctcgacggc gagcgggaag tggatctgca cgccgaggat tcggcctcca tccggctcga
     2401 cagggacggc ccccgcgtgg tggacgtgcc cgccgcgttg cgttgggctg cggagagcgg
     2461 cttcttcggc aataaagagc tcgtgcggcg gcttgcgccg ctgtgacgat acgtagacac
     2521 agcgcccggg gtctcctctt tcatgagcgg cctcgggctt tttataggcg ggcgggagaa
     2581 agacgcttcg gcccgcaggc aggctgaaag agaggcgaga agatggctgt agaaattgtg
     2641 atgcccaaga tggggctgac gatgaccgtc ggttccgtcg gaaagtggct gaaaaaggaa
     2701 ggcgatcagg tgacgaaggg agagcatgtc gtggaggtgc tcacggagaa gatcacgaac
     2761 ctcgtcgacg ctcccgcgga cggcgtgctg cgcatcgcgg cgcccgaggg gacgacgctt
     2821 cccatcggcg gcgtgatggg gtacgtcggc gcggctggcg aggcgctgcc cgggctgccg
     2881 cagcaggagc cgcagagcgt tgccgcaaca gtatcgcaga gtagggaggg ggcagcgccg
     2941 cttccgccgt ccgctggggg cgcgccggga gggggattcc ccgcggagcg cccggcgcac
     3001 gtcagaatat cgcccgtggc gcggaagatg gcgcaggagc acaacatcga ctacgcgcgc
     3061 atacagggaa cagggccgga cggccggatc acgaaagagg acatcgaggc ggtgctcgcg
     3121 tctccggccg ccgctccgca ggcccctctc cccgccgccg gattcgacgc tgctccgcgc
     3181 cccgatgggg tttcatgggc cgcgccgctt cctggctcct tcgaggaacg cccgtactcc
     3241 ggggtgcggc ggatcatcgg cgaccggatg ctcgaaagct ggagcgccgc gccgaaggtg
     3301 acccatcatg cacgggcgga tgcgaaggcg cttctggagc tgcgcgcctc catcaacgag
     3361 acgctggaga aggaggagcg cgtcagcgtg acggacatgc tggtgaagat cacggccatg
     3421 gcgctccggc agaagctctt catgaacgtc tcgctccgcg gcgaccgcat cgttacgttc
     3481 cgcgaggtgc atctgggcgt cgccgtggcg ctcgaaggat cgctgatcgt accggtgatc
     3541 cggaacgccg acgagaagag cctgacccgc atcagccgcg agctgaagga tctttcaagg
     3601 agggcgaggg agaacgcgct ggctcccgag gagatctccg gaggcacctt cacgctgacc
     3661 aa
//
LOCUS       DAOTIL010000182         3652 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminobacteriaceae bacterium isolate AMR_MDS_3026
            NODE_30050_length_3652_cov_4.910481, whole genome shotgun sequence.
ACCESSION   DAOTIL010000182 DAOTIL010000000
VERSION     DAOTIL010000182.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868050
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminobacteriaceae bacterium (wastewater metagenome)
  ORGANISM  Aminobacteriaceae bacterium
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae.
REFERENCE   1  (bases 1 to 3652)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3652)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:19:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,224
            CDSs (total)                      :: 3,198
            Genes (coding)                    :: 3,184
            CDSs (with protein)               :: 3,184
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3652
                     /organism="Aminobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_30050_length_3652_cov_4.910481"
                     /isolate="AMR_MDS_3026"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:3034609"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1070
                     /locus_tag="P5201_04965"
     CDS             <1..1070
                     /locus_tag="P5201_04965"
                     /inference="COORDINATES: protein motif:HMM:NF018234.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HRV97917.1"
                     /translation="AFYAASRIRDGDAGDWQREWAELAGRVEARGETSLAAGHLVSAR
                     DQLLRAAYYYRLSLVSMLPDNPAFKERGAKVRELFQKGGALFDPPVEYFEVPFEGTSL
                     PGYFRKAASGDRPVKTLLMIGGGETFAEDLFFYIAHQAHARGYNFATVDLPGQGLLPL
                     EGMVFRTDTQAPVKAVVDVLVKRPDVDTERLSAFGISGGGLFVPQAAMFDPRIKAVAM
                     CSAVVDARPLFATMPAALDTPEERAGWTSFHEGVVKSICWRYGVPMDHPEKLIEANEG
                     NTFDPAKVAAPALIIVGEGEYRSLEVQRQQKIALDNFPNPSKKMVVTPSDEGASNHCV
                     MENRALIGQVLFDWLDETLGE"
     gene            complement(1172..1333)
                     /locus_tag="P5201_04970"
     CDS             complement(1172..1333)
                     /locus_tag="P5201_04970"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRV97918.1"
                     /translation="MISLKKAVFFLCVDPIDHVASWIPGDLVKYVRESKMQGEDASRA
                     HPPLEEPPL"
     gene            1592..2044
                     /gene="alaE"
                     /locus_tag="P5201_04975"
     CDS             1592..2044
                     /gene="alaE"
                     /locus_tag="P5201_04975"
                     /inference="COORDINATES: protein motif:HMM:NF018333.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0034639 - L-amino acid efflux
                     transmembrane transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-alanine exporter AlaE"
                     /protein_id="HRV97919.1"
                     /translation="MTGTPGVNNKRKQISFLIADITAMILFSTGLCMIIEIFVAGLSF
                     FQSVGARIAAVPVNLITGRPYGWFRDTLFLTLGIDRSSPVKLFFGDTLAFVVFQVPLY
                     VLVLLFAGATWKQIAVTSLSASLVFSLTGRPYGIFLDFCRRIAVKLLG"
     gene            2117..3097
                     /locus_tag="P5201_04980"
     CDS             2117..3097
                     /locus_tag="P5201_04980"
                     /inference="COORDINATES: protein motif:HMM:NF024242.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HRV97920.1"
                     /translation="MNGRMYEWRRQIQAIVDAIDECIKNHDDEALTLRRLSCGLGYSE
                     FHTTRKFKEISGMQFREYLRGRRLAFALKEVRDSGKSLLDIAFDYGFSSHEAFTRAFK
                     GTYGVAPGEYRKNPKPVVLRTKINPFDRYFLGLGEIGMMKSADDVKIYCVTIPAHKFV
                     HIKNYESNGYWDFWQKQSSIPGQDCETICGLLDSIKGKLDDDGGSEPDSGSGQIMAYI
                     NDPDGRLCDWGIPRTECYGARLPLDYKGEVPPQMLLIDVPEAEYIVFEHGPFDYEQEN
                     RSVEEKIEHAMAAFDYADAGYLLDTSPGRITYFYHNPGQFFKYVRPVRRV"
     gene            complement(3194..>3652)
                     /locus_tag="P5201_04985"
     CDS             complement(3194..>3652)
                     /locus_tag="P5201_04985"
                     /EC_number="2.7.7.-"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase domain-containing
                     protein"
                     /protein_id="HRV97921.1"
                     /translation="ENNYVAFSFIWLRSEKRRLKTPYAIIGYIGKSVTLQKLCRSAAS
                     DESEPMNMLREESLRFIRALAEEYGAARVFLFGSCLLLPQEDARDIDLAVEGISEEQL
                     DAFWDRLMWADALGRKPVDVLRLEDGHWLNPIILDEGVLIYEAQLSPIAL"
BASE COUNT          801 a         1053 c         1053 g          745 t
ORIGIN      
        1 aagcgtttta cgccgcgtcc cgcattcggg acggagacgc gggcgattgg cagcgcgaat
       61 gggcggaact ggccggccga gtcgaagcca ggggagaaac atcccttgcc gcagggcatc
      121 ttgtaagcgc cagggatcag ctcttgagag ccgcctacta ctaccgcctc tcgctggtct
      181 ccatgctgcc ggacaatccc gccttcaaag agcggggagc caaggtccgg gaactcttcc
      241 aaaagggcgg cgcgctgttc gatcctccgg tggagtactt cgaagtcccc ttcgagggta
      301 cgtcgcttcc cggatacttc cgaaaagccg cttccggcga ccgtcccgtc aagaccctgc
      361 tcatgatcgg cggcggtgag accttcgcgg aggatctctt cttttatatc gctcaccaag
      421 cccacgcccg aggctacaat ttcgccacgg tggaccttcc cggacaggga ctgcttcctc
      481 tggagggaat ggtcttccgc acggacacgc aggcgcccgt gaaggccgtt gtggacgtcc
      541 ttgtcaagag gccggacgtg gatacggagc gtctgtccgc cttcggcatc agcggaggcg
      601 gactcttcgt tccgcaggcg gcgatgttcg acccgcgcat caaggccgtc gccatgtgct
      661 ccgcggtggt cgacgcccgc cccctcttcg ccaccatgcc cgccgccttg gatactcctg
      721 aagagcgagc ggggtggacc tccttccatg aaggcgtggt gaagagcatc tgctggcgct
      781 acggagtgcc gatggaccac cccgaaaaac tgatcgaggc gaacgaggga aacaccttcg
      841 atccggcgaa agtcgccgcg cccgctctga tcatcgtcgg cgagggagaa taccggagcc
      901 tggaagtgca gcggcagcag aagatcgctc tggacaattt tccgaatccc tcgaagaaga
      961 tggtcgtcac gccgtcggac gagggcgcga gcaatcactg cgtcatggaa aaccgcgccc
     1021 tgatcggcca agtgcttttc gactggctcg acgagacgct gggcgagtga aagaggcgca
     1081 ggtatcgggc tcgccatcgc ggacacggct cacgcaggcc catggtcgga gactctcccc
     1141 ggagagcgaa gagggcgggg ggagccccgc ttcacagcgg aggttcttca aggggagggt
     1201 gcgcccggga ggcgtcctcc ccttgcatct tggattcccg gacatactta acgaggtctc
     1261 cgggaatcca ggatgcaacg tgatcgatag ggtcgacgca cagaaaaaat acggcttttt
     1321 ttaacgagat cacaacgaat ccctcctttt tcgcactctc tgctttcgtg gcattatcgc
     1381 ccgcaagagc gcttcgcgtc aactcgcagg cgtccagatg cgtaatggcg aagcggcaag
     1441 cagtccacga acgggagaga tgattttccc gcttctcttg gaaaacgaac cccattctgc
     1501 gtgctacaat gacaggcgtt gtagaaaaat gcagttgttt ttaaggaaac aaaaaatctt
     1561 ctgcactcac gactttgaag aggtgaattc tatgaccgga acccccggcg tgaacaacaa
     1621 gaggaaacaa atcagctttc tgatcgccga cattaccgcc atgatcttgt tctcgacggg
     1681 cctctgcatg atcatcgaga tcttcgtcgc gggactctcg ttcttccagt cggtcggggc
     1741 gagaatcgcg gctgttccgg tcaacctgat caccggaagg ccgtacggat ggttcagaga
     1801 cacgctcttc ctgacgctcg gcatcgacag gtccagtccg gtgaaactct ttttcggcga
     1861 cacgctggcg ttcgtcgtct ttcaggtgcc gttgtacgtc ctcgtcctgc tctttgctgg
     1921 ggccacatgg aaacagatcg ccgtcacctc cctctccgcg tcgctggtct tctcgctgac
     1981 cggacggccg tacggcatct ttctggattt ttgcaggcgg atcgccgtca aactcctcgg
     2041 gtgaaacgaa cagcaagtaa aatcaagcgg cgcgctcccc gtctccctac aatacgttgc
     2101 gggtgggcgg aacggggtga acgggcgaat gtacgagtgg cggcggcaaa ttcaagcgat
     2161 cgttgatgca atcgacgagt gtatcaaaaa ccatgatgac gaagccttga cgctgcgccg
     2221 tctttcgtgc ggattggggt attccgaatt tcataccacg aggaaattca aagagatttc
     2281 gggtatgcag ttcagggagt atctgcgggg gagaagactg gccttcgcgc tcaaagaggt
     2341 tcgggacagc gggaagagtc ttctggatat cgcgttcgac tacggcttct cgtcgcacga
     2401 agcgttcacc agagccttca aaggaacgta cggcgtcgct ccgggcgaat accggaaaaa
     2461 ccccaagccc gtcgtccttc gcacgaaaat caaccctttc gaccgctact ttttaggact
     2521 gggagagatc ggcatgatga aatccgcgga cgatgtgaaa atttactgcg tcaccattcc
     2581 cgcgcataag tttgtgcaca tcaaaaacta cgagagcaac ggctattggg acttttggca
     2641 aaaacagagc agcattccgg gacaggactg cgaaacgatt tgcggcctgc tcgacagcat
     2701 caagggcaaa ttggacgacg acggcggaag cgaacccgac agcggcagcg gccagattat
     2761 ggcgtacatc aacgacccgg acggcagact ctgcgattgg ggcattccgc gaacggagtg
     2821 ttacggcgcg cgccttcctc ttgattacaa aggagaagtg ccgccgcaga tgcttctgat
     2881 cgatgttccc gaggccgagt atatcgtctt cgaacacggg ccgttcgatt acgagcagga
     2941 aaaccggagc gtggaggaga agatcgaaca cgcgatggcg gcgttcgatt atgcggacgc
     3001 cggatacctc cttgacacct cccccggaag gataacgtac ttttaccata atcccgggca
     3061 gttttttaag tacgtcaggc ctgtgcggag ggtatagaat caacggctcg tccgctgcgg
     3121 tcaacaagca tcggtctgca tggaaagagc gcactccgtg caggccgatg gctctctgtt
     3181 cttttcgagc agtctataga gcgattggcg atagctgggc ttcatagatg aggactcctt
     3241 cgtccaggat tatagggttc agccaatgcc cgtcctccag acgaagcaca tcgaccggct
     3301 tgcgtccgag cgcgtccgcc cacatcagtc tgtcccaaaa cgcatccagc tgctcttcgg
     3361 agattccctc caccgcgaga tcaatatcgc gcgcgtcctc ctgaggcagc aagaggcagg
     3421 agccgaacaa aaaaacgcgg gctgccccgt actcctccgc caacgcacgg atgaacctca
     3481 ggctttcttc tctcagcata ttcatcggct cgctttcatc actcgccgca ctccggcaaa
     3541 gtttctgcag ggtaaccgat ttcccgatgt atccgatgat agcataaggc gttttcagtc
     3601 tgcgtttttc ggaacgtaac cagataaagg aaaaagcaac ataattgttt tc
//
LOCUS       DAOTIL010000183         3620 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminobacteriaceae bacterium isolate AMR_MDS_3026
            NODE_30375_length_3620_cov_3.957924, whole genome shotgun sequence.
ACCESSION   DAOTIL010000183 DAOTIL010000000
VERSION     DAOTIL010000183.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868050
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminobacteriaceae bacterium (wastewater metagenome)
  ORGANISM  Aminobacteriaceae bacterium
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae.
REFERENCE   1  (bases 1 to 3620)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3620)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:19:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,224
            CDSs (total)                      :: 3,198
            Genes (coding)                    :: 3,184
            CDSs (with protein)               :: 3,184
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3620
                     /organism="Aminobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_30375_length_3620_cov_3.957924"
                     /isolate="AMR_MDS_3026"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:3034609"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            249..1502
                     /locus_tag="P5201_04990"
     CDS             249..1502
                     /locus_tag="P5201_04990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013048608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine deiminase family protein"
                     /protein_id="HRV97922.1"
                     /translation="MKVSVTSEIGRLRAVIMQPPGKGIERCTPLNVGALAWDSVPSAS
                     KAAEEHKKWVAAVEGFGAGVFLFEDLLRDVLALPSVKNELVAAVLETEKAFLTERTLD
                     ILREHLLGLSPSALVEQLFFGMTKDELHRSSGEIALRDLVDRTSEWCLFPMTNVLYSR
                     DPAAVLGEGVVFGKMFNRDRVKEPACIRAIFRHHPEFRDSEFSSWYGEEPEDTTPVEG
                     GNVHCYSRNVFVIGVNERTHPDSIEKIARRTMRCGEITDVLALLFENQRLGPADSIGL
                     TVHVDMFLNMIDHDAFLFFPYIEKKLTVLHITRGRGDRLRIEREDSLFGAIQKVLKLD
                     AVRVIKVGGEESEAVAFSEQRAGSGGNTFNLEPGRVCIWDRNVATIKALEKGGIKVVP
                     LDADELVKGGGGPRCSTMPLWRDDV"
     gene            complement(1523..2293)
                     /locus_tag="P5201_04995"
     CDS             complement(1523..2293)
                     /locus_tag="P5201_04995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005659333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase/isomerase family protein"
                     /protein_id="HRV97923.1"
                     /translation="MTTVLSSIKEGVCRIELNRPEAMNTFNEELAIGLDEAFRAADAN
                     DEVRVIIVSGAGKHFSTGIDLRELLSKERHEIPGFLKLMDLHNHRLASLTKPVIASVH
                     GYALANGTGLALACDFVVASEDAVFGTTAVNVGLICLEPGYQLARWIGEKRALQYVLS
                     GELIPASKGLELGFVHTMTARETLEEKTLELAKKLASKSALSLRAGKVGFARMEGLAL
                     ERAIQAGGDIFAALAASEDGREGVEAFLAKRPPLFKGR"
     gene            complement(2306..>3620)
                     /locus_tag="P5201_05000"
     CDS             complement(2306..>3620)
                     /locus_tag="P5201_05000"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: protein motif:HMM:NF015284.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="L-serine ammonia-lyase, iron-sulfur-dependent,
                     subunit alpha"
                     /protein_id="HRV97924.1"
                     /translation="FECMLEHAPLLGLDARFELHPFPEADHPNSSLLILTGENGKEIT
                     LHSRSVGGGSFEIASLNGYHVSITGENFETLIETSSGSDSSVLSIFGERASSSRIHHT
                     NKGVLFHLSTSSPVEPVMLDTLAALPGTVRLFSAPPVMHPLGGDMLFSSAEEAIIMAE
                     RDGLTLGEAALRYEAALLGRDESSILSEMEERLEIMLNAVHLGLSGDFSPMKLLLPVA
                     GTLAAAERKGALFLGGPHARAAIRSMAALHTNASGGVVCAAPTGGAAGVLPGVLTTLT
                     EDLKISRKAVLMALWAAGGIGLFHDIRSTFAAEVAGCQVEIGAAGAMAAAAVVEAAGG
                     SARQGCDAAATVFQNMMGSVCDLVQGVVEIPCHSRNAALASQAFLCADLVLGGYRSPV
                     PLDETIDAVDSTGRMLPEELRCTSRGGLALCPSAQRLPRLDKKIS"
BASE COUNT          762 a         1002 c         1069 g          787 t
ORIGIN      
        1 ctttgtgccc agcagaggcg gcataagtca caatcctgtg gagtacactc cggacaagca
       61 gctccttcag ggtgttcatg tcctgctcga cgtcgcggcc gggcgtcttc tcaaggaagg
      121 aaacaagacg gaggttgagc agaagagtct gtagggcttc atgtttttca agcatctgat
      181 tccgggaaat acgaacgaac gcatgttctt atctctgact ttgtatccac ccgcaggagg
      241 gagatagagt gaaggtcagc gttacttccg aaataggacg gcttcgcgcc gtcatcatgc
      301 agccgccggg caaggggata gaacgatgca cgccgttgaa tgtcggcgct ctcgcgtggg
      361 attcagtgcc gagcgcttca aaggcggcgg aagagcacaa gaaatgggtg gcggcagtgg
      421 agggattcgg cgccggagtt ttcctcttcg aggacctgtt gcgcgacgta ctcgcgttac
      481 cctctgtgaa gaacgaactg gttgctgctg ttttagagac tgaaaaggcc tttctcacgg
      541 aacggacgct ggatattctc cgtgagcatt tactcgggct ttctccaagc gcgcttgtgg
      601 agcagctctt tttcggtatg acgaaggatg agctgcaccg ttcttcggga gagatcgcac
      661 tgcgcgatct ggttgaccgg acaagcgagt ggtgtctttt tccaatgacc aatgtgctct
      721 attcccgtga tcctgccgct gtgcttggag agggcgttgt ctttggaaag atgttcaacc
      781 gggacagggt gaaagagccg gcatgcatca gggctatctt ccggcatcat cccgagtttc
      841 gcgactcgga attttcctcg tggtacggcg aagaaccgga ggacacgact cctgtcgagg
      901 gaggaaacgt ccactgttac agcagaaacg tcttcgtcat cggagtgaac gagcgcacgc
      961 acccggattc catagaaaaa atcgcgcgga ggacgatgcg ctgcggggag attaccgatg
     1021 tgctggcgct gctcttcgaa aaccagcgtc tcggccctgc ggactccatc ggcctgaccg
     1081 tccatgtcga tatgtttttg aacatgatcg accatgacgc gtttctcttc ttcccctata
     1141 tagaaaaaaa actcactgta cttcatatca cgaggggaag aggggatcgc ctgcggattg
     1201 agagggagga ttctctgttc ggggccattc aaaaagtgct caagctggac gcagtccgcg
     1261 ttatcaaggt tggcggcgag gagagcgagg cagtcgcctt ttccgagcag cgcgcaggtt
     1321 cgggcggaaa tacgtttaac ctcgaaccgg ggcgtgtgtg catctgggac agaaacgtcg
     1381 cgaccatcaa ggcgcttgaa aaaggcggga tcaaggtcgt tccgcttgac gcggacgaac
     1441 ttgtgaaagg cggcggaggt ccccgatgct cgacgatgcc gctgtggagg gacgacgtgt
     1501 aagccgtccc tccacaggga tgtcagcggc ctttgaagag gggagggcgc ttggcgagga
     1561 aggcttcgac accttccctc ccgtcctcgg atgcggcgag tgcggcaaag atatctccgc
     1621 cggcctgtat cgcgcgctcg agagcaagcc cctccatgcg agcgaatcct acctttcccg
     1681 cacggagcga aagcgcgctc ttcgacgcaa gtttttttgc cagttccagc gtcttctcct
     1741 caagcgtttc tcttgccgtc attgtatgga cgaacccgag ttcgagcccc ttcgatgcgg
     1801 ggatgagttc gccggagagg acgtactgta gcgcgcgttt ctctcctatc caacgggcaa
     1861 gctgataccc tggctcgagg cagatgagcc cgacgttgac cgctgttgtt ccgaagaccg
     1921 catcctcgga tgcgacaaca aaatcacagg caagtgcgag acccgttccg ttggcgagcg
     1981 catatccatg aacggaagct ataaccggct tggtcaacga tgcgagcctg tggttgtgca
     2041 gatccatgag cttcaagaag ccggggattt cgtgccgctc tttcgagagc agctcccgga
     2101 ggtcgattcc cgtcgaaaag tgcttacctg ctccggaaac gatgatgacg cgaacctcgt
     2161 cattcgcgtc ggccgcacgg aacgcctcgt caagaccgat cgcaagctct tcgttgaatg
     2221 tgttcatcgc ttccggcctg ttcagttcga tacggcaaac gccttctttt atgctgctga
     2281 gcactgttgt catttttttc tcccttcagc ttattttttt gtcgagccgt ggaaggcgct
     2341 gcgccgacgg acagagagcg agaccgcctc ttgatgtgca acgcaactct tcggggagca
     2401 tccttcctgt gctgtcgacg gcgtcgatcg tttcgtccag aggcaccggg cttcggtacc
     2461 cgccgagcac aagatccgcg cagagaaacg cctgcgatgc cagcgcggcg tttctgctgt
     2521 ggcagggtat ttcaaccact ccctgcacca ggtcgcagac ggatcccatc atgttttgaa
     2581 atactgtcgc ggctgcgtcg cacccctggc gcgcgcttcc tccggcggcc tctacaactg
     2641 cggctgcggc catcgctcct gccgcgccta tctctacctg gcatcccgca acttctgcgg
     2701 cgaaagtact tctgatatcg tgaaagagtc ctatgcctcc ggcagcccaa agcgccatga
     2761 gtacagcttt cctgctgatt ttaaggtcct ctgtgagcgt tgtcagaacg ccgggaagca
     2821 cgccggccgc gccgcctgtg ggagctgcgc agaccactcc tccagaagcg tttgtatgca
     2881 gcgccgccat agagcggata gcagcccgtg cgtgaggccc tccaaggaag agggctccct
     2941 tgcgttccgc ggctgcaagc gttcccgcga caggcaagag aagcttcata ggtgagaagt
     3001 ctccggaaag accgagatgt actgcgttga gcatgatctc caaccgctct tccatctccg
     3061 atagtatcga ggattcatcg cgcccaagca gggccgcctc gtaacgaagc gccgcttctc
     3121 cgagtgtgag tccatctcgc tcggccatga taattgcctc ttccgcactg gagaaaagca
     3181 tatcccctcc aagcggatgc attactggag gcgcagaaaa aagacgaact gttcccggca
     3241 gagcggcgag tgtgtcgagc atgaccggct caaccggcga agatgtagac aggtgaaaga
     3301 gaaccccttt gtttgtatga tggatccgac tggaggaggc tcgttctccg aagatggaaa
     3361 gcaccgaaga atcgcttccg gatgaggttt caatgagcgt ttcaaagttt tcgccggtga
     3421 tcgagacgtg atagccgttc agcgaggcta tctcgaaaga accgccgccg accgatctgc
     3481 tgtgaagcgt gatctctttc ccgttttctc cggtaaggat gagcagcgag ctgttgggat
     3541 gatcggcctc gggaaaagga tgcagctcaa aacgcgcgtc gagcccgaga agaggcgcat
     3601 gctccagcat gcattcaaaa
//
LOCUS       DAOTIL010000184         3602 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminobacteriaceae bacterium isolate AMR_MDS_3026
            NODE_30581_length_3602_cov_4.101212, whole genome shotgun sequence.
ACCESSION   DAOTIL010000184 DAOTIL010000000
VERSION     DAOTIL010000184.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868050
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminobacteriaceae bacterium (wastewater metagenome)
  ORGANISM  Aminobacteriaceae bacterium
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae.
REFERENCE   1  (bases 1 to 3602)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3602)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:19:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,224
            CDSs (total)                      :: 3,198
            Genes (coding)                    :: 3,184
            CDSs (with protein)               :: 3,184
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3602
                     /organism="Aminobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_30581_length_3602_cov_4.101212"
                     /isolate="AMR_MDS_3026"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:3034609"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1372
                     /locus_tag="P5201_05005"
     CDS             <1..1372
                     /locus_tag="P5201_05005"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_process: GO:0009966 - regulation of signal transduction
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HRV97925.1"
                     /translation="VTIEELFQVLSEHLFPVIPFRCQTLFIKRSREAASFEVLDSGGD
                     VLRSMQTDFLEKKKGILGYVANTGKAYLMGDVSIDPYFIPHSPEVRSAVVAPVMYKGF
                     LWGAIVLDSDRKFAFGIRERDMLSIVGSYVALHLEEFFARQELDKKATQLRFLHRVIQ
                     QIAAERNNEALARKIVDVLFDELGFPGAGFYTPPPKGENAPVLVAGRSRAERETCSPE
                     CELEAGAAMRSRTPVEREVNGRALVLSIPVTFEGEVFGALAARHDAGFSAADRELLEI
                     TAEHMTTFWALNNLLAQRRHESLVDPLTQVWNRRYIMHRLDEESSRINRTNGRGSVIL
                     VDLGDFKQINDRYGHVTGDEVLRVTSATMSENLRNYDMIGRYGGDEFLLYLPDVSREE
                     AGAIMVRMELKVAEQKIEGFEGAVILDYGIAGCPEDGINLSEVIRIADERMYRYKAER
                     KAVKSE"
     gene            1369..1812
                     /locus_tag="P5201_05010"
     CDS             1369..1812
                     /locus_tag="P5201_05010"
                     /inference="COORDINATES: protein motif:HMM:NF013098.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HRV97926.1"
                     /translation="MIVSLHHASFTVENLERSVAFYQETLGLKLEGVWERDQEYSEGV
                     TGIRGAWLKVAYFSLPNASFELVEYLGAKGKKIDTSTNNVGSAHVCLIVANFDAFVEA
                     LAERGVVFAGKTCTIPAGSNKGKKIAYIEDPDGNTLELVSSRALS"
     gene            1864..2058
                     /locus_tag="P5201_05015"
     CDS             1864..2058
                     /locus_tag="P5201_05015"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRV97927.1"
                     /translation="MLGQYYLITGIGAFALILLAVITGLFGRNLRKIVPGPLVLKIHK
                     FSALTGALCALLHVLGVHGY"
     gene            2145..3149
                     /locus_tag="P5201_05020"
     CDS             2145..3149
                     /locus_tag="P5201_05020"
                     /inference="COORDINATES: protein motif:HMM:NF014069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HRV97928.1"
                     /translation="MPSQKILVVEDSLSQRMMISRHLRNWEYEVLEAADGVEAFTAFR
                     QQSPAIIITDLDMPEMNGFQLIEEIRRKEIARTYIIVLSASDDKSSVVSALALGADDY
                     LVKPYHPDELRVRLTGAERVLRLQNQELLIFAMAQMVDTRSPETGAHLDRVQYFSRRI
                     AEELAAEGDPLVTASWIAQLFSLSPLHDIGKIGIPDAILNKPGRLTPEEFVIMKEHVT
                     IGASIFSRIYERTHYAPFGFAIDIIRCHHEKWDGSGYPDGLAGESIPLCGRIVALADV
                     YDAISSKRVYKPSFPREQCWDIINRGRGSHFDPRLVDIFNRNEDYFWNIVEELRDIDD
                     "
     gene            3183..>3602
                     /locus_tag="P5201_05025"
     CDS             3183..>3602
                     /locus_tag="P5201_05025"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRV97929.1"
                     /translation="MYGQFSRFVGRISLRRIIRIAFVFQILIPVSFLGWMFFRTSRES
                     VEALSMRLGSEVAARIAEHLQSYMAVPLLVNSLNADALESGRVDIASPRIWQPFFTER
                     IRAFPSIAYTFIGTPRGEFFGARRFKENVELFVASPET"
BASE COUNT          772 a          982 c         1060 g          788 t
ORIGIN      
        1 cgtgacgata gaagaacttt tccaggtgct ctccgaacat cttttcccgg tgattccgtt
       61 ccgctgccag acgctcttca tcaagcgctc gcgtgaagcc gcttcgttcg aagtgctcga
      121 ctcgggagga gatgtgctgc ggtcgatgca gacggatttc ctcgagaaga agaagggtat
      181 tctcggatac gtcgccaaca cgggcaaggc ctacctcatg ggcgatgtgt ccatcgatcc
      241 ctatttcata ccccattcgc ccgaggtgcg gtcggccgtc gtcgcccctg tgatgtacaa
      301 gggattcctc tggggagcta ttgtgctgga cagcgacagg aagtttgctt tcggcatacg
      361 ggagagggat atgctgagca tcgtgggttc ctacgtcgcg ctccacctcg aggagttttt
      421 tgccagacag gaactggata agaaggcgac gcagctgcgc tttctccaca gggtgatcca
      481 gcagatcgct gcagagcgaa acaacgaggc gctcgcacgc aagattgtcg atgtgctctt
      541 cgacgagctc ggtttccccg gagccggatt ctacactcct cctccgaagg gagagaacgc
      601 tccggttctg gttgccggca gatcgagggc ggagcgagaa acctgttccc cggagtgcga
      661 acttgaggcg ggagctgcta tgagaagccg cacccccgtc gagcgggaag tgaacggcag
      721 agcgctcgtt ctctccattc cggtcacttt tgaaggagag gtcttcggcg ctctcgccgc
      781 ccggcacgac gccggttttt ccgcagcgga ccgggagctg ctcgagatca cggcagagca
      841 catgacgacc ttctgggcgc tgaacaatct gcttgcgcaa cgccgccacg agtcgcttgt
      901 cgatcctttg acgcaggtat ggaacaggcg gtacatcatg caccggcttg acgaagagag
      961 ttcccgcatc aacaggacga acggcagagg ctccgtgatt ctcgtcgacc tcggcgactt
     1021 caagcagatc aacgaccgtt acgggcacgt tacgggggac gaggtccttc gtgtgacatc
     1081 cgccacaatg agtgaaaacc tcaggaatta cgatatgata gggcgctacg gcggagatga
     1141 gttccttctc tacctgccgg acgtctcacg agaggaagcc ggggcgatca tggtacggat
     1201 ggagctgaag gtcgccgagc aaaaaatcga aggctttgag ggagcggtca tccttgatta
     1261 cggcatagcc ggctgccctg aggatggaat aaacctctcg gaagtcatca ggattgcgga
     1321 tgaaaggatg tacagataca aggcggagag aaaggcggtg aagagtgaat gatcgtatcg
     1381 ctgcatcatg ccagttttac ggtggagaat ctcgaaaggt cggtcgcgtt ttatcaagaa
     1441 acgctggggc tgaaactcga aggagtgtgg gagcgggatc aggaatactc cgagggcgtc
     1501 accggcatac gcggggcatg gctcaaagtg gcctattttt ctcttccgaa cgcttctttt
     1561 gaacttgtgg agtacctggg ggcgaagggg aagaagatcg acacgtcgac aaataatgtc
     1621 ggaagcgctc atgtgtgcct tattgttgca aatttcgacg ccttcgttga ggcgcttgcg
     1681 gaacgcggcg tcgtttttgc agggaaaacc tgcactatcc ctgccggttc gaacaaggga
     1741 aagaaaatcg cctatatcga ggaccccgac ggaaataccc ttgagctggt gtcatcgaga
     1801 gctctttcgt aacacgccgg atgcgaaaac cggaaagaac cagcaaagga ggataaaaac
     1861 gtcatgcttg gtcagtatta tttgattacc gggataggtg cttttgctct gattctcctc
     1921 gccgtgatca ccgggttgtt cggcagaaac ctcaggaaga tcgtccccgg gccccttgtg
     1981 ctgaaaatcc acaagttctc ggcccttaca ggcgctcttt gcgctcttct gcacgtgctc
     2041 ggcgttcacg ggtattagca gcgtccgcct gccggcttca caacgcgtct ttattctccg
     2101 gcgtcacagt ttaccataaa gaaaggtgat tttcagtgct tcctatgcca agtcagaaga
     2161 tcctcgtcgt cgaggactcg ctcagtcagc gaatgatgat ctcccgccac ctccgtaact
     2221 gggagtacga ggtgctggag gccgccgacg gcgtagaggc gttcaccgcg tttcggcagc
     2281 agagccccgc gatcatcata acggacctcg atatgccgga aatgaacggc tttcagctta
     2341 ttgaggagat tcgcaggaag gagatagccc gtacgtacat catcgtcctc agcgcctccg
     2401 acgacaagtc gagcgtcgtt tcagccctgg cgctgggcgc ggacgactat ctcgtgaagc
     2461 cgtatcaccc cgatgaactg cgcgtgcgcc tgacaggagc cgagcgcgtg ctgcgcctcc
     2521 agaaccagga gctgctcatc ttcgccatgg cacagatggt ggacacccga agtcccgaga
     2581 caggagccca cctcgacaga gtgcagtatt tcagcaggcg catagcggaa gagcttgccg
     2641 cggaaggaga tccgcttgtt acggcttcgt ggatagcgca gcttttttcc ctttctccgc
     2701 ttcatgatat cggcaagatc ggcatccccg acgccatcct gaacaaaccg ggcagactga
     2761 ctcccgaaga gtttgtcatt atgaaagagc atgttaccat cggcgcctcg atcttctcgc
     2821 ggatctacga acgtactcac tatgcgccgt tcggattcgc catcgatatt atccgctgtc
     2881 atcacgagaa gtgggacggc agcggatacc ccgacgggct cgcgggagag agcattccgc
     2941 tctgcggaag gattgtcgct ctcgccgacg tgtacgacgc catcagcagc aagcgtgtct
     3001 acaaaccatc cttcccgcgg gagcagtgct gggatattat caacaggggc aggggaagcc
     3061 atttcgatcc gcggcttgtc gacatattca accgcaacga ggattacttc tggaatatcg
     3121 tcgaagagct acgggatatc gacgattgag ttcgccgtag gtgacgggag ggggatcttt
     3181 ttatgtacgg acagttctcc cgttttgtcg ggcgtatttc gctccggagg atcattcgca
     3241 tcgcttttgt ctttcagata ctcattcctg tctctttctt ggggtggatg ttcttcagaa
     3301 cgtcccggga atcggtcgaa gcgctctcta tgcgcctggg gagcgaagtg gcggcgcgga
     3361 tcgcggagca tctccagtcg tatatggctg tccctcttct cgtgaactct ctcaatgcgg
     3421 acgcgctgga gtcggggagg gtcgacatcg cctctccccg gatctggcag ccctttttca
     3481 ccgaacgcat cagggccttt ccgtcaatag cgtatacctt catcggcacg ccccgcggag
     3541 agttcttcgg ggcgcgccgc ttcaaggaga atgtcgaact cttcgtagca agtcccgaga
     3601 ca
//
LOCUS       DAOTIL010000185         3600 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminobacteriaceae bacterium isolate AMR_MDS_3026
            NODE_30603_length_3600_cov_4.028773, whole genome shotgun sequence.
ACCESSION   DAOTIL010000185 DAOTIL010000000
VERSION     DAOTIL010000185.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868050
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminobacteriaceae bacterium (wastewater metagenome)
  ORGANISM  Aminobacteriaceae bacterium
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae.
REFERENCE   1  (bases 1 to 3600)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3600)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:19:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,224
            CDSs (total)                      :: 3,198
            Genes (coding)                    :: 3,184
            CDSs (with protein)               :: 3,184
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3600
                     /organism="Aminobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_30603_length_3600_cov_4.028773"
                     /isolate="AMR_MDS_3026"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:3034609"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..258
                     /locus_tag="P5201_05030"
     CDS             <1..258
                     /locus_tag="P5201_05030"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013700.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M23 family metallopeptidase"
                     /protein_id="HRV97930.1"
                     /translation="VASRPGKVVFSGTIQGFGRTVTIDHGDGMKTRYSHNSANLVKKG
                     DVVRQGQPIAKVGRTGRATCTHVHFSVLINGKPINPEKYLR"
     gene            complement(360..1127)
                     /locus_tag="P5201_05035"
     CDS             complement(360..1127)
                     /locus_tag="P5201_05035"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRV97931.1"
                     /translation="MTGFRRIVCALFAVCCVLSLPAVAAEDASARFDELAARYFARME
                     GREDAVALRTALEAEAVQADEFALWRSLFREGLAPREGASNAITLLNALLDGGDPARW
                     ESASGFFLPSFVPKPLAAADAVYAAALFLLRMDGKGASDLAGMLFERLLDSPKGKYFF
                     ISAAPAEYREIVRMLTERGVVPSFGKWPEAAVEGTLPLGAPVRGRVSRDRAISEGMTF
                     LNGAGQPAPNGMYAWDRKKGRIYDVVDVERRRFWFYD"
     gene            1395..>3600
                     /locus_tag="P5201_05040"
     CDS             1395..>3600
                     /locus_tag="P5201_05040"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HRV97932.1"
                     /translation="MNIQRADSLSELVERVRSLEPFLDSLGDIVSLVDPGMRYLWASA
                     SMEAALALLRSEIVGGECFRLHHAVSSVCPHCPVSEAMSTGQVVEKELYSKERKKHFL
                     TRGIPVFGPDDKIIAAFEVTRDITDMKTRHLETEAKERYFRYLFENAPVGIFQISMDG
                     KYIAANARQALILGYESPEELMEVVNRTSVKEAHYSSSESREDVLKNLMEHPNLWTTR
                     ETEFRRRDGLLIDVQLHHRMVFSPEGEMLHIDGYMEDISERKSLERKIQQQLDIQQKI
                     FDAVPLHLYLKDEKGLYAVVNQSYADFKGIPKDEFTGKTVFDIINGHNAVIAKAEDDQ
                     VLATGEALLAEERIARGHNGIRQWHRVIKNPLRDAEGRIIGIVECSVDITQLKEALDA
                     LRESEQRFRSLAEDMPLMISTTLADGTILYANKAYCTCFGVRRDELLGRSFLEFLVEE
                     DRKEARESIAALMPDAPISIHEERVVVADGTLRWHRWMNRAFFDSEGRAQFLQAVGED
                     ITERKLSDEALLAAKDQAQRASAAKSEFVANVSHEIRTPMNGILGLSELLLETELKDE
                     QRDFARMIHTSASNLLGVLNDILDFSKVEAGRFELNILPFSPANLVRESAELFSPQAA
                     SKELSLNLRLDGALPPHLLGDPVRLRQILINLLSNAVKFTKEGSVCVSAKLLRDDEKS
                     ADILFSVADTGPGIPDALLADIFTPFKQGEAFLNRRYGGTGLGLAISSRFVEM"
BASE COUNT          797 a         1114 c         1030 g          659 t
ORIGIN      
        1 gtcgcgtcgc gccctggcaa ggtcgtcttc tccggaacaa tccaggggtt cggccggacg
       61 gtgaccatcg accacggcga cgggatgaag accaggtaca gccacaacag cgccaacctc
      121 gtcaaaaaag gggacgtcgt ccgtcagggg cagcctatcg cgaaggtggg cagaaccgga
      181 agggcgacct gcacgcacgt tcacttcagc gtccttataa atggtaagcc gatcaacccg
      241 gagaagtacc ttcgctgagc ggaaacttcc tttatcttca aagaaaagaa ggcgcgtccc
      301 gaaaacaggg aacgcgcctt cttccttata aatggaacta caagaaagct gcataaggat
      361 cagtcgtaga accagaagcg gcgccgctcc acgtcgacga cgtcgtaaat tctccccttc
      421 ttcctgtccc acgcatacat tccgttcggc gcaggctgtc cggcgccgtt taaaaaggtc
      481 attccctccg agatcgccct gtcgcgggat acgcgcccgc gaaccggggc ccccaacggc
      541 agcgtcccct ccacggcggc ctccggccac tttccgaagg agggaacgac gcctcgctcc
      601 gtcagcatcc ggacgatttc gcgatactcc gccggcgcgg cggaaatgaa gaagtacttt
      661 cctttcgggg agtccagcag gcgctcgaag agcataccgg cgagatccga cgcgcctttc
      721 ccgtccatcc gcagcagaaa gagcgcggcg gcgtagacgg cgtcggcggc ggccagcggc
      781 ttcgggacga aggaggggag gaagaacccg gaggcgctct cccaccgcgc aggatcccct
      841 ccgtcgagaa gcgcgttcag aagggtgatc gcgttcgaag cgccctccct cggagcaagc
      901 ccttcgcgga agagggagcg ccacagagca aactcgtcgg cctgcacggc ctccgcttcc
      961 agggcggtcc gaagcgcaac agcatcctca cgcccttcca ttctggcgaa gtagcgcgcc
     1021 gccagctcgt cgaaacgcgc cgacgcatcc tccgcggcga ccgcaggcaa ggagagaacg
     1081 cagcacactg caaaaagagc gcagactatg cgccgaaaac ccgtcatgag catcaactcc
     1141 ttcagctcct ttagagacat cgaattatct taggtgcttc atccattcta ccactcaaaa
     1201 ttagtcagta atgatcaatc attatatact cttctttcgc gtctgaaaac ccggcggagc
     1261 gcaaaaaacc cgccgtgaag cggaaaactc cccgatggtt tcacttttcg cgcgcaatga
     1321 tcacatccct ctctctttcc cgtgctctat cctgtagaat atacgtatga tgcgggaaag
     1381 ggagcgatag aggaatgaac attcaacggg cggactctct cagcgaactg gtcgaacggg
     1441 tacgttccct ggagccgttt ctcgactccc tcggggatat cgtctccctt gtcgatccag
     1501 ggatgcgcta cctctgggca tccgcttcga tggaagccgc tctcgctctc ctccgttcgg
     1561 agatcgtcgg gggggagtgt ttccgccttc atcacgccgt ttcctccgtc tgcccgcact
     1621 gtcccgtcag cgaggccatg tcgacggggc aggtcgtcga aaaagagctc tactccaaag
     1681 aacgaaagaa acactttttg acccgaggaa ttcccgtctt cggtccggac gacaagatca
     1741 tcgcggcttt cgaagtcacc cgcgatatca cggatatgaa gactcgtcac cttgagacgg
     1801 aggcgaagga gcgctatttc cgctacctct tcgagaacgc acccgtcggg atcttccaaa
     1861 tttccatgga cggtaaatat atcgcggcga acgcgcgcca ggcgctcatc cttgggtatg
     1921 agtctccgga ggagctcatg gaggtcgtca acagaacatc cgtcaaggaa gcgcattact
     1981 cttcctccga gtccagggaa gacgttctga aaaatctcat ggagcatccg aacctgtgga
     2041 ccacccggga gaccgagttt cgcagacgcg acggcttgtt gatcgacgtc caacttcacc
     2101 acaggatggt cttttctccg gagggggaga tgctccacat cgacggctac atggaagata
     2161 tctcggaaag aaaatccctg gagcgaaaga tccagcagca gctggacatc cagcagaaga
     2221 tcttcgacgc ggtgccgctg cacctctatc tgaaggacga gaaggggttg tacgcggttg
     2281 tgaatcagtc gtacgccgac ttcaagggaa ttccgaagga tgagttcacc gggaaaacgg
     2341 tattcgacat catcaacgga cacaacgccg tcattgcaaa ggccgaagac gatcaggtgc
     2401 tggccaccgg agaagcgctt ctggcggagg agcggatcgc caggggacac aacggcatca
     2461 ggcagtggca cagggtcatc aagaaccctc tccgcgacgc cgaggggagg atcatcggca
     2521 tcgtcgagtg cagcgtcgac atcacacagc tcaaggaggc cctcgacgcg ctccgggaga
     2581 gcgaacagcg cttccgttcg ctggcggagg atatgcccct gatgatctcc accacgcttg
     2641 ccgacggaac catcctctac gcgaacaagg cgtactgcac ctgcttcggc gtcagaaggg
     2701 acgaacttct cgggcgctct ttcctggaat ttcttgttga ggaggacaga aaggaggcgc
     2761 gcgaatccat cgccgcgctg atgccagacg cccccatctc catccatgaa gagcgcgttg
     2821 tcgtcgccga cggcacgctc cgctggcacc gctggatgaa ccgcgcgttc ttcgactcgg
     2881 aggggagggc gcagtttctt caggccgtcg gagaggatat cacggagcga aagctctccg
     2941 atgaggcgct cctcgccgca aaagatcagg cgcaacgagc gagcgcggca aaatccgagt
     3001 tcgtcgccaa cgtgagccac gagatccgca cgccgatgaa cgggattctc ggcctctcgg
     3061 agcttctgct ggagacggag ctgaaggacg agcagcggga cttcgcgcgg atgatccaca
     3121 caagcgcctc caatctgctc ggcgtgctca acgacatcct cgacttttcg aaggtcgaag
     3181 cggggcggtt cgagctgaac atcctcccgt tttccccggc caacctcgtc agagagtcgg
     3241 cggagctctt ctccccccag gccgcttcca aggagctctc gctcaatctg cggctggacg
     3301 gcgccctccc cccacatctg ctcggcgatc cggtccgcct gcgccagatt ctcatcaatc
     3361 tgctctcgaa cgcggtgaag ttcacgaaag agggaagcgt ctgcgtctcc gcgaagctcc
     3421 tccgcgacga tgaaaagagt gccgatattc tcttctccgt cgcggacacg ggtcccggaa
     3481 ttccggacgc gcttctggcg gatatcttca ccccgttcaa gcagggggag gccttcctga
     3541 acagacgata cggcggcacc gggctcggac tggctatttc aagccgtttc gtcgagatga
//
LOCUS       DAOTIL010000186         3562 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminobacteriaceae bacterium isolate AMR_MDS_3026
            NODE_31049_length_3562_cov_3.196749, whole genome shotgun sequence.
ACCESSION   DAOTIL010000186 DAOTIL010000000
VERSION     DAOTIL010000186.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868050
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminobacteriaceae bacterium (wastewater metagenome)
  ORGANISM  Aminobacteriaceae bacterium
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae.
REFERENCE   1  (bases 1 to 3562)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3562)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:19:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,224
            CDSs (total)                      :: 3,198
            Genes (coding)                    :: 3,184
            CDSs (with protein)               :: 3,184
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3562
                     /organism="Aminobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_31049_length_3562_cov_3.196749"
                     /isolate="AMR_MDS_3026"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:3034609"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..393
                     /locus_tag="P5201_05045"
     CDS             <1..393
                     /locus_tag="P5201_05045"
                     /inference="COORDINATES: protein motif:HMM:NF014684.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRV97933.1"
                     /translation="GGIGVAGYLVVAMMISGAFAGLAGAVEIAGVHRRAIEGITGGYG
                     FSGIVVALFGGLHPAGIIPASFFFGLLIVGADMTQRMVGVPANMVLVLQGIIILVIVA
                     TRMVLANPYLMERVWRRYQNLTRRDKEA"
     gene            398..1342
                     /locus_tag="P5201_05050"
     CDS             398..1342
                     /locus_tag="P5201_05050"
                     /inference="COORDINATES: protein motif:HMM:NF014684.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HRV97934.1"
                     /translation="MDFVVNIFALGIPFSVALLLASLGEMFNQRAGVFNLGCEGIMAM
                     GAFLGMLVPYSVGQGGPVSGAYNILGLLLAMGIGALLGLFFGLVVVTFRAPQGIAGIG
                     LQMFGVGAAGTLFRHFVGGTQSIPGIDRLPIPVLSEIPVLGPVLFSHNPLVYMTFLMV
                     PAAWYVLFKTPWGLRVRASGTNPRAADSIGIKVNLVRFQALAVGGALAGLAGAYLSLC
                     QVKMFSDEIIAGRGFIAVALVYFGHWRPLKIMGGALLFSLAQALQLAIQGQGIDFPYE
                     FAVMLPYAMVIVVLAFSRESLLLGPTALGKPFNREMRT"
     gene            1420..1905
                     /locus_tag="P5201_05055"
     CDS             1420..1905
                     /locus_tag="P5201_05055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009200603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0019239 - deaminase activity [Evidence
                     IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside deaminase"
                     /protein_id="HRV97935.1"
                     /translation="MNTSHETLLRRCVEISKEAVAAGNHPFGALLADKDGNILVESGN
                     IEVTERDCTGHAETTVMRLAGKKYSKEFLWECTLYTTAEPCCMCVGAIYWGNVGHVVF
                     GITEKQLLELTGSHEDNPTFDLPSREIVARGQKKIEIIGPVADEALQEEIIAVHRGYW
                     K"
     gene            2004..2447
                     /locus_tag="P5201_05060"
     CDS             2004..2447
                     /locus_tag="P5201_05060"
                     /inference="COORDINATES: protein motif:HMM:NF037100.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase domain-containing
                     protein"
                     /protein_id="HRV97936.1"
                     /translation="MPLMTNSSPVCSSAAMDSSWTKRRNNAIRRRCEQFACSLETLFG
                     DDLLAVWLYGSCATKTNHAESDVDVAIILTEAGKKRYEQNIDGIASDCDDLEFSMARE
                     LAMPVSLLIWSEERLSREISFCTGFASRLLEKNVVLFQKRGWMPK"
     gene            2444..2974
                     /locus_tag="P5201_05065"
     CDS             2444..2974
                     /locus_tag="P5201_05065"
                     /inference="COORDINATES: protein motif:HMM:NF017020.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HEPN domain-containing protein"
                     /protein_id="HRV97937.1"
                     /translation="MMVKTLTGANDSPEIRERLVLKALRVSLGHLNGCVLMIELNEAV
                     MANDHLCYALFHAAKAALFARGKDPSTHQGVKMELSGVASENGLDREFIRFFGNRERL
                     RSEMSYSYLGEVETFSIQEVTTALQASHRWMKTLIDELVRPLIPSVPQFDELVRSSET
                     GNSLLGDKGLWSGFHE"
     gene            complement(3172..>3562)
                     /locus_tag="P5201_05070"
     CDS             complement(3172..>3562)
                     /locus_tag="P5201_05070"
                     /inference="COORDINATES: protein motif:HMM:NF013696.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HRV97938.1"
                     /translation="QDTIEAAIRRVIAGLCAAGRCEFELDYRRNLPATVNDHEVTMTA
                     KAVCEYVLGADKVKETELIMGSEDFSYFEQKVPGTFFFLGTGNPEKETNNPHHHPRFD
                     LDEDALPAGSAMLAGFAWAQLTGEVRG"
BASE COUNT          674 a         1098 c         1070 g          720 t
ORIGIN      
        1 ggcggcatcg gcgtggcggg gtatctcgtc gtcgccatga tgatcagcgg cgcgttcgcc
       61 ggactcgcag gggccgtcga gatagccggc gtgcaccgcc gcgccatcga gggaatcacc
      121 ggcggctacg gcttcagcgg catcgtcgtc gccctcttcg gagggctgca cccggcgggc
      181 atcatcccgg cctccttctt cttcgggctg ctgatcgtgg gagccgacat gacgcagcgc
      241 atggtgggcg tcccggcgaa catggtgctc gtgctgcagg gcatcatcat tctcgtcatc
      301 gtggcgacgc gaatggtgtt ggcgaacccc tacctgatgg aacgggtctg gcggcgctac
      361 cagaacctga cccgccgcga caaggaggcg taatccgatg gacttcgtcg tcaacatttt
      421 cgcgctcggc attccgttca gcgtggcgtt gctgctcgca tccctcgggg agatgttcaa
      481 ccagcgggcg ggcgtcttca atctcggctg cgaaggcatc atggcgatgg gcgccttcct
      541 cgggatgctc gtcccgtaca gcgtcggtca gggaggcccc gtctccggag cctacaacat
      601 actcggcctc ctgctcgcca tgggaatcgg cgcgctgctc ggcctcttct tcggcctggt
      661 cgtggtcacc ttccgcgccc cgcagggcat cgcgggcata gggcttcaga tgttcggcgt
      721 aggcgccgca ggaacgctct tccgtcactt cgtcggcggc acgcagagca ttcccggcat
      781 cgaccggctg ccgatccccg tcctttcgga gattccggtg ctcgggccgg tcctcttctc
      841 gcacaatccg ctggtctaca tgacgtttct catggttccc gccgcgtggt acgttctctt
      901 caaaacaccg tgggggctgc gcgtccgcgc ctcgggcacc aaccctcggg cggccgattc
      961 catcggcatc aaggtcaacc tcgtccgctt ccaggcgctc gccgtcggag gcgcgctcgc
     1021 agggctcgcc ggggcgtacc tcagcctctg ccaggtgaag atgttcagcg acgagatcat
     1081 cgccggacgc ggcttcatcg ccgtggcgct cgtctacttc ggccactggc gtccgctgaa
     1141 gatcatgggg ggcgcgctcc tcttcagtct cgcgcaggcg ctccagctcg ccatccaggg
     1201 gcagggaatc gacttcccgt acgagttcgc cgtcatgctc ccctacgcga tggtcatcgt
     1261 cgtcctcgcc ttcagccgcg agagcctgct actgggcccc accgcgctcg gcaagccgtt
     1321 caaccgcgag atgcgcacct aggaaaaaag acggtcattg cagcacatcc ggaggggagc
     1381 cgcgcgctcc cctcgttttt acttgaggag gagaagacaa tgaacacatc gcacgaaacg
     1441 cttctgagac ggtgcgtcga aatatcaaag gaggctgtcg ccgcgggaaa ccaccccttc
     1501 ggcgcgctgc ttgccgacaa agacgggaac atcctcgtcg aatcgggaaa catcgaggtc
     1561 actgagcggg actgcacggg gcacgcggag accaccgtca tgcgcctcgc cggaaaaaag
     1621 tactccaagg aattcctgtg ggagtgcacc ctctacacca ctgccgagcc gtgctgcatg
     1681 tgcgtcggcg cgatctactg gggaaacgtg gggcatgtgg ttttcggcat caccgagaag
     1741 cagctcctcg aactcaccgg ctcgcacgag gacaacccca ccttcgacct cccctcccgc
     1801 gagatcgtcg ccaggggaca gaagaaaata gagatcatcg gccccgtcgc cgacgaagcg
     1861 ctccaagagg agatcatcgc cgtccacagg gggtattgga agtagaggcg cgccttcttg
     1921 gcgcctttcg atgcacgaag agcgcgcagt gtataataaa cttgatcaag ggctgcggag
     1981 aactcttgga agggggcgga gcaatgccgc tcatgacaaa cagctcgcct gtgtgttcat
     2041 ccgccgcgat ggactcgtcg tggacgaaaa gaagaaacaa cgcaatccgt cgtcggtgcg
     2101 aacaattcgc ctgctcactt gaaacccttt tcggggacga tctgctcgcc gtctggttgt
     2161 acggctcctg tgcgacgaag acgaatcatg cggaatccga cgtggacgtt gccatcatcc
     2221 tgaccgaggc gggaaagaaa cgctacgagc agaatatcga tggcatcgcc tccgactgcg
     2281 atgatctcga attttcaatg gcgagagaac tcgcgatgcc cgtctcgctt ctgatatgga
     2341 gcgaagagag gctttcgcga gagatctctt tttgcacggg cttcgcctct cgtttgctgg
     2401 aaaaaaacgt tgtgctgttc caaaaacggg ggtggatgcc gaaatgatgg tgaaaacgct
     2461 cacgggagcg aacgattctc ctgaaatcag ggagcggctg gttctcaagg ccctccgcgt
     2521 ctctctcggc catttgaacg ggtgcgtgct catgatcgaa ctgaacgagg ctgtgatggc
     2581 gaacgatcac ctctgctacg cgctgtttca tgcggcgaaa gcagcgcttt ttgcaagagg
     2641 caaggatcct tccacgcatc aaggggtgaa gatggaactc tccggggtag cgtccgaaaa
     2701 cggattggac cgtgagttca tccgtttttt cggaaatcgt gaacgattgc gctcggagat
     2761 gagctactct tatctgggcg aagttgagac cttttccatc caggaggtaa ccactgccct
     2821 gcaagcctcc caccgctgga tgaagacgct gatcgatgaa cttgttcgcc cgctgattcc
     2881 ttccgttccg caattcgacg aacttgtgcg atcttcggaa acagggaact cccttctcgg
     2941 cgacaaagga ctctggtcgg gttttcacga gtgacggcgg gttgccctgg aggaggcaac
     3001 agcgacagac ttcaaaaaga gcgtccggac ggagaaaaac agcgtatgaa gagtctttgc
     3061 ggctcgcttt ccgcgtcggg atcaacctct atcaaaatat gccgtaggcg cttcgtcctc
     3121 gggagttgtc tgccctcggg cgaaacgccg tcaaaatctc cggaaatcgg ttcagccccg
     3181 cacttccccc gtcagctgcg cccacgcgaa ccccgcgagc atggcggaac cggctgggag
     3241 cgcgtcttcg tccaggtcga atctcgggtg atggtgaggg ttgttcgtct ccttctccgg
     3301 attgcccgtt ccaaggaaga agaaggttcc ggggactttc tgctcgaagt agctgaagtc
     3361 ctccgaaccc atgatcagct ccgtctcctt caccttgtcc gcgcccagaa cgtactcgca
     3421 gaccgccttc gccgtcatcg tgacctcgtg atcgttgact gttgcgggaa gattgcgcct
     3481 gtagtccagt tcgaactcgc agcgtccggc ggcgcacagc cccgcgatca ccctcctgat
     3541 ggccgcttct atcgtgtcct gt
//
LOCUS       DAOTIL010000187         3556 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminobacteriaceae bacterium isolate AMR_MDS_3026
            NODE_31126_length_3556_cov_3.113396, whole genome shotgun sequence.
ACCESSION   DAOTIL010000187 DAOTIL010000000
VERSION     DAOTIL010000187.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868050
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminobacteriaceae bacterium (wastewater metagenome)
  ORGANISM  Aminobacteriaceae bacterium
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae.
REFERENCE   1  (bases 1 to 3556)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3556)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:19:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,224
            CDSs (total)                      :: 3,198
            Genes (coding)                    :: 3,184
            CDSs (with protein)               :: 3,184
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3556
                     /organism="Aminobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_31126_length_3556_cov_3.113396"
                     /isolate="AMR_MDS_3026"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:3034609"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..284)
                     /gene="flgN"
                     /locus_tag="P5201_05075"
     CDS             complement(<1..284)
                     /gene="flgN"
                     /locus_tag="P5201_05075"
                     /inference="COORDINATES: protein motif:HMM:NF016985.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0044780 - bacterial-type flagellum assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar export chaperone FlgN"
                     /protein_id="HRV97939.1"
                     /translation="MASPSSLGDALTKQAEILRTLGEVTVRQREALKEGRLELLQDLF
                     REQQAAGFAAEAAEERRRRNAADLAATLGCEPRVSSIRSRLSDNEAYQLK"
     gene            complement(287..574)
                     /gene="flgM"
                     /locus_tag="P5201_05080"
     CDS             complement(287..574)
                     /gene="flgM"
                     /locus_tag="P5201_05080"
                     /inference="COORDINATES: protein motif:HMM:TIGR03824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis anti-sigma factor FlgM"
                     /protein_id="HRV97940.1"
                     /translation="MIDKIPGVYGPGVMDKLNAKKGYKPSREGGKGDGVEFSSFAVAL
                     AKVSGELKKIPDVREQLVEDFKRQIAEGTYSPDMNQVARSLIGAGLLNRED"
     gene            complement(616..1266)
                     /locus_tag="P5201_05085"
     CDS             complement(616..1266)
                     /locus_tag="P5201_05085"
                     /inference="COORDINATES: protein motif:HMM:NF012383.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosyltransferase family protein"
                     /protein_id="HRV97941.1"
                     /translation="MSTLEELFRFAAHLFWPRACPFCGALGESACVLCLLPCLEPPMP
                     MELGSLPLWSGGEHDGVLRELVLALKYGGDRSLGVAMGRALGRIFPRPDADLLVPVPL
                     HRGSERAYNQSLALAAGAAKEWGIPAKEGLVWSERREEQTSLGQEERKMMPRDVFSPV
                     TKSVAGKRIVLVDDVATTGTTLERAAETVRRGGGDPVLALSWTCAPRTKERMRGVR"
     gene            complement(1253..1672)
                     /locus_tag="P5201_05090"
     CDS             complement(1253..1672)
                     /locus_tag="P5201_05090"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005661771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HRV97942.1"
                     /translation="MNGGYFFYDSKNAVLASELANLYRFTRWGRSRSVEDIDRMLRGT
                     SMCFSVRYEEHLVAFCRILTDFIYRGSLWDIMVHPDHQGKKVGTALLDYALNHPAIKP
                     IPLIITYTSELAPFLVPHGFAPKEGALMLLRRPIEYS"
     gene            complement(1669..1887)
                     /locus_tag="P5201_05095"
     CDS             complement(1669..1887)
                     /locus_tag="P5201_05095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014162839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase"
                     /protein_id="HRV97943.1"
                     /translation="MLIVACQICGETKVIAGTPDGDGMARATWICPVCGTGQVMQLPV
                     SSDARKSDLRSIVCGMALATHGGMEERP"
     gene            complement(1909..3075)
                     /locus_tag="P5201_05100"
     CDS             complement(1909..3075)
                     /locus_tag="P5201_05100"
                     /inference="COORDINATES: protein motif:HMM:NF022727.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3084 domain-containing protein"
                     /protein_id="HRV97944.1"
                     /translation="MQIQIWSDMNWRLILTLVLLSGVLAYLGDVLGMRIGKRRISLFG
                     LRPRDTSRVITAFTGILISIAILITMTVISDNVRTALFSMKFIQGQLQTLTRDLQKSR
                     DEAELMAINLVGSETKLQEQQKKLAGVQAELESVAPLLDEARASLVRTQTEKEQVEQE
                     KAALSRSVEELKEEAEALRKGLIQVRSGRISVFAMEILAQAPVEPMSSRSDVERIFQE
                     LRKQAEFVVAARTGQPSADVLLSVDLEKEAERIAECSGQPSRKFVRAIAEGNAVFGED
                     IRVVYEVYDSVLVYRRGELLTRRTLKPYEGINAETELHLILRMVNRKAVADDIKPDPV
                     TGNVGTLDGAQFFEAVDRLTRADGPVLVEVTAAEDIHTEGPVRVSIEIRPAEEN"
     gene            complement(3139..3555)
                     /locus_tag="P5201_05105"
     CDS             complement(3139..3555)
                     /locus_tag="P5201_05105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006301237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YkgJ family cysteine cluster protein"
                     /protein_id="HRV97945.1"
                     /translation="MWWEEGLRFTCLGCGRCCRGEPGAIFFTPEEEEKIATFLSLPVS
                     EFRRRYVTSRWRAPSLKEKNNGDCVFYDAAADRCFIYRVRPLQCRLFPFWPALLESEE
                     AWNEAAALCPGMNRGDFHTAREIRGLLGLNPFPDLL"
BASE COUNT          659 a         1091 c         1065 g          741 t
ORIGIN      
        1 tgtttgagtt gatacgcctc gttatccgac aggcgcgagc ggatcgagga gactctgggc
       61 tcgcatccaa gcgtggccgc cagatcggcg gcatttcgtc tgcgccgctc ttcggcggct
      121 tccgcggcga accctgccgc ctgctgctcg cggaagaggt cctgtaataa ttcgaggcgc
      181 ccttctttga gcgcctctct ctgccgtaca gtaacttcac cgagcgtgcg cagaatttcc
      241 gcctgcttgg tcagcgcgtc tcccagagag gacggtgagg ccacggttaa tcctcccggt
      301 tcagcagccc cgcgccgata aggcttcgcg ctacttggtt catatccggt gagtaggtcc
      361 cctcggctat ttgtcgctta aagtcttcaa caagctgctc gcgaacgtcg gggatttttt
      421 tcagctcgcc ggaaacttta gcgagcgcca cggcgaagga gctgaactct actccgtcgc
      481 ccttgccccc ctcgcgggaa ggcttgtacc ctttcttcgc gttgagcttg tccattacgc
      541 ctggtccgta cacgccggga atcttgtcaa tcatcttcat caccccgaaa gaagaaactt
      601 tttctcttca ctttgttatc ggacgccgcg catcctctct ttagtccggg gcgcgcaggt
      661 ccacgaaagt gcaaggacgg gatcgccccc gccgcgacgt actgtttcag cggctcgttc
      721 caaggttgtg ccggttgtgg cgacgtcgtc cacaaggacg attctctttc ccgcaacgct
      781 cttcgtcaca ggcgaaaaga cgtcgcgggg catcattttc cgttcctcct gccccagaga
      841 ggtctgttct tcacggcgtt cgctccagac gagcccttct ttggcaggaa tcccccattc
      901 ttttgccgcg cctgcggcaa gagcaagaga ctggttatag gctcgttcgc tcccccggtg
      961 cagcgggacg ggaacgagca ggtcggcgtc cggacggggg aagatgcgcc ccagcgcacg
     1021 gcccatagcg acgccgagcg accggtctcc tccgtacttc agcgcgagga caagctcccg
     1081 gagcacgccg tcgtgctctc cgcccgacca gagcgggagg ctcccaagct ccatcggcat
     1141 cggaggctca agacagggca acagacagag aacgcacgcg gactcgccga gcgcgccgca
     1201 gaaggggcac gctctgggcc agaaaaggtg cgcggcaaac cggaaaagct cctcaagagt
     1261 actcaatcgg ccgcctcagg agcatgaggg cgccctcttt cggggcgaag ccgtgcggaa
     1321 cgagaaaggg ggcgagttcg ctggtatagg tgatgatcag cggaatcggc ttgatcgccg
     1381 ggtggttgag cgcatagtcg agcagcgcgg ttcccacttt ttttccctga tgatcagggt
     1441 gtaccatgat gtcccagaga gatccccggt agataaagtc ggtcagtatc ctgcagaaag
     1501 cgacaaggtg ctcctcgtaa cgcacgctga agcacatcga ggtgccgcga agcatgcggt
     1561 cgatgtcctc gacggagcgg cttcgccccc atcgggtaaa gcggtagaga ttggccagct
     1621 cggaggcgag caccgcgttc ttgctgtcgt aaaaaaagta gccgccgttc acggcctttc
     1681 ctccatacct ccgtgcgtcg caagggccat accgcagacg atggagcgga gatcggattt
     1741 ccgggcgtcg gaggagaccg gaagctgcat gacctgcccc gtgccgcaaa ccggacagat
     1801 ccaggtggct cgggccattc cgtcgccgtc gggcgttccg gcgatgacct tcgtttctcc
     1861 gcaaatttga caggcaacta tgagcattgc gcattcctcc acagggaact aattttcttc
     1921 ggcagggcgt atctcgatgg agacgcgtac cggaccttcc gtatgaatgt cctcggccgc
     1981 ggttacctcg acaagcaccg gtccgtccgc ccgtgtgaga cggtccacgg cttcaaagaa
     2041 ctgcgccccg tcgagcgttc ccacatttcc ggtgacgggg tcgggtttga tgtcgtcggc
     2101 gaccgctttc ctgttcacca tccgaaggat gaggtgcagc tcggtctccg cgttgattcc
     2161 ttcgtagggt ttgagcgtcc gccgggtcaa aagctctcct cttctataga cgagaacgct
     2221 gtcgtacact tcgtagacga cgcggatatc ctccccgaaa accgcatttc cctcagctat
     2281 cgcgcggacg aacttcctcg aaggctgacc ggaacactcg gcgatacgct ccgcctcttt
     2341 ttccagatcg acggaaagga gcacgtcggc ggacggctgg ccggttctgg cggcaacgac
     2401 gaactccgcc tgtttacgca gctcctggaa gatgcgttcg acgtcgctgc ggctgctcat
     2461 cggctcgacg ggcgcctgag cgagtatttc catggcgaag acggagatgc ggccgctgcg
     2521 tacctggatg agccccttcc gcagcgcttc cgcttcctcc ttgagctctt cgacggagcg
     2581 tgagagcgcg gccttctcct gctccacctg ctctttttcc gtttgagtgc ggacaaggct
     2641 tgcgcgggcc tcgtcgagca ggggcgcgac gctctccagc tccgcctgga ctccggcgag
     2701 cttcttctgc tgctcttgga gtttggtctc gctgccgacg aggttgatcg ccatgagctc
     2761 cgcttcgtcg cggctttttt gaagatctcg tgtgagcgtc tgaagctgtc cctgaatgaa
     2821 tttcatactg aagagggcgg tgcggacgtt gtccgatatg accgtcatgg tgataaggat
     2881 ggcgatggag atgaggatgc cggtgaaggc ggtgatcacc ctgctggtgt cgcgtgggcg
     2941 aagcccgaag agagagatgc ggcgtttgcc tatacgcatt ccaaggacat ccccgaggta
     3001 cgcgaggacg ccgctgagga ggaccagcgt gagaatgagc ctccagttca tgtccgacca
     3061 gatttggatc tgcatgcgca ctcccctcga tcttcttccg ctgaaacgtc cggacgcgtg
     3121 ttcgcgtcaa cgaaactatt atagcaggtc gggaaaggga ttgagcccga gcagcccgcg
     3181 aatctccctt gccgtatgaa aatctccccg gttcatgccc gggcacagcg cggcggcttc
     3241 attccacgcc tcttcggatt caagcagggc gggccaaaag gggaagagac ggcactggag
     3301 cggccggacc cggtagatga agcacctgtc ggcggccgcg tcgtagaaga cgcagtcccc
     3361 gttgtttttc tccttcagac tgggcgctct ccatctcgag gtgacatacc ggcggcgaaa
     3421 ctcactcacc ggaagagaga ggaaagttgc tatcttttcc tcctcttccg gcgtgaaaaa
     3481 gatcgctccc ggttctccgc ggcagcagcg gccgcatccg agacaggtga aacggagtcc
     3541 ttcctcccac cacact
//
LOCUS       DAOTIL010000188         3542 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminobacteriaceae bacterium isolate AMR_MDS_3026
            NODE_31288_length_3542_cov_3.630054, whole genome shotgun sequence.
ACCESSION   DAOTIL010000188 DAOTIL010000000
VERSION     DAOTIL010000188.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868050
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminobacteriaceae bacterium (wastewater metagenome)
  ORGANISM  Aminobacteriaceae bacterium
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae.
REFERENCE   1  (bases 1 to 3542)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3542)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:19:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,224
            CDSs (total)                      :: 3,198
            Genes (coding)                    :: 3,184
            CDSs (with protein)               :: 3,184
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3542
                     /organism="Aminobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_31288_length_3542_cov_3.630054"
                     /isolate="AMR_MDS_3026"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:3034609"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(50..952)
                     /gene="aroE"
                     /locus_tag="P5201_05110"
     CDS             complement(50..952)
                     /gene="aroE"
                     /locus_tag="P5201_05110"
                     /EC_number="1.1.1.25"
                     /inference="COORDINATES: protein motif:HMM:TIGR00507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004764 - shikimate 3-dehydrogenase
                     (NADP+) activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate dehydrogenase"
                     /protein_id="HRV97946.1"
                     /translation="MKNETALQQSHALGTVEIRKNVELLVGLLGDPVAHTLSPAMQNA
                     AFQALGAGYRYLAFRVRAEDFEHAMEGLAALGAVGTNITVPHKERAFRWARRLDPSAS
                     RTGAVNTVLFSEEGGTGYNTDVAGIRTALAALSPRRDGALILGAGGAARAAVCAMQDE
                     GFSHIAIMNRTRERAEKLQAQLFPAKSCKLPTCEILSFEEAPSFTPDLLVNTTSLGMH
                     DTPWPEELLDTIVSLAKGGAVLDLVYAHDRETPLCDRAQQAGIPSVSGQEVLLHQGTA
                     AFKLFTGMAAPVEIMRAALQKGNI"
     gene            1372..2076
                     /locus_tag="P5201_05115"
     CDS             1372..2076
                     /locus_tag="P5201_05115"
                     /inference="COORDINATES: protein motif:HMM:NF019947.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain-containing protein"
                     /protein_id="HRV97947.1"
                     /translation="MKNDTPVSSGNREFLEQYIPLLDFFAEVCGPEYELVLHDVSRPE
                     SSIVAIRNGQISGRTVGSTMSEYAPTFIKLIQSGGYAEDMVAHVDRTKDNRILESHTF
                     FIKDEKGELRGMICANHDVTSLIKLHDTLHEKIRMLNGLSKDLAERTNEDPPFEAILE
                     KESHSNLNGLMDVLIEQASSEFSVAPPQMSPEERIRFVSMLKTRHLFSMKGAVAKVAE
                     RLGVSEATVYRYLKKA"
     gene            2244..>3542
                     /locus_tag="P5201_05120"
     CDS             2244..>3542
                     /locus_tag="P5201_05120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008711054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent enzyme"
                     /protein_id="HRV97948.1"
                     /translation="MRELMTGNEAIARGAWEAGCHVAAAYPGTPSTEILENVSRYKEI
                     YSEWSTNEKVSLEVVSGASMAGARAMCAMKHVGLNVAADPFFTMSYIGVNGGLVIVDA
                     DDPGLFSSQNEQDNRWYALHAKLPMLEPSDSQECLDFMKAAFDISERFDTPVLLRVTT
                     RICHSKSVVETGERMERLIREYTRNPAKNAMLPVFAKAKHYLVEKRLLDLREFNETSP
                     FISYVEGDNEIGVLTSGIGYQHSREALGDRVSYLKLGMTWPLPEKAIRDFASKVKNLW
                     IVEENDAFIEGAVRGMGIACSGKDALPLVDELSPALIAKAILGTKPSSGPDTGIAPPN
                     RPPLLCAGCPHRGLLFAMSKKKGLVVMGDIGCYGLGSMPPLNVGESTICMGAGFSAVV
                     GFQKAQEKAGRSQKVFGILGDSTFFHSGITGLIDAVVNKSR"
BASE COUNT          798 a          912 c         1028 g          804 t
ORIGIN      
        1 ttcgtgagtg cggggcatta gtaaacgtct cgcactcacg aaatgagctt tatatatttc
       61 ctttttgcag ggctgccctc ataatctcca ctggagcggc cattccggtg aacaacttga
      121 aggctgccgt tccttgatga agcagcactt cctgaccgga gacggacggt atgcccgcct
      181 gttgcgcgcg atcgcagaga ggtgtttcac gatcgtgtgc atacacgagg tcgaggacgg
      241 cgcctccctt tgccagggaa acgattgtgt cgagcagctc ctccggccag ggagtatcgt
      301 gcattccgag cgacgtcgtg ttcacaagca gatcgggagt gaaagaggga gcttcttcaa
      361 acgagagtat ttcgcatgtc gggagtttgc agctcttcgc ggggaagagc tgcgcctgaa
      421 gcttttcagc ccgttcacgt gtgcggttca taatcgcgat atgtgagaat ccttcgtctt
      481 gcatcgcgca gacggccgcg cgcgccgcgc ccccggcgcc gaggatgaga gcgccgtccc
      541 tgcgggggga gagtgcggca agtgccgtac ggatgccggc gacgtccgtg ttgtagcctg
      601 tgccgccctc ttcggaaaaa agcaccgtgt tgacggctcc tgtcctggat gcggagggat
      661 ccaaacggcg cgcccagcgg aatgcgcgtt ccttgtgggg tacggtgatg ttcgtcccca
      721 cggccccaag agcggccagt ccctccatcg cgtgttcgaa gtcctctgca cgaacccgga
      781 atgcaagata gcggtaccct gcgccgagag cctgaaatgc cgcattctgc atggcaggcg
      841 aaagggtatg ggcgactggg tctccaagca gcccgacaag aagctcaacg ttttttcgga
      901 tttcaacggt tcccagcgcg tgtgattgct ggagtgcggt ctcattcttc atggctttct
      961 cctccttggc ctacgggccg gacgtatact aaaaaagccg gaacccgaaa ccgggcccgg
     1021 ccttttgttt tttctctgtc gacggttttg cgctacatga ctccgtggac gctctggctg
     1081 cgggagaacc ggtttccata cgaatgctga ccgtcggcgt aataatacca acggtcgtag
     1141 ctaaaggaaa aaccggaaac tccgcggggt gaaagacgtc tcaagatcat gaaaacctgc
     1201 tttctcccat caagggtgtc tgaaagttgc gggtaataat accccccttt cagtgaggtg
     1261 tcaatacacg cccgctcaac tcggccgtaa aacttcttta cagaatccgc aggacgtgat
     1321 atgaattttt tatcacaacg ttgtgtattt tttgaaggga aggaacgctt catgaaaaat
     1381 gatacgccgg tctcttcggg gaaccgtgag tttcttgagc agtatattcc ccttctggat
     1441 ttttttgccg aagtgtgcgg tccggagtac gaactcgtgc tgcacgatgt gtcgcgtccc
     1501 gagtcgtcca ttgtagccat ccggaatggc cagataagcg gaagaacagt ggggagcaca
     1561 atgtcggagt acgcgccgac atttatcaaa ctcatccaaa gcggcggata cgccgaggat
     1621 atggtggccc atgtcgacag gacgaaggat aatcgaatcc ttgagtctca tacctttttt
     1681 ataaaggatg agaaggggga gctgcgcggc atgatatgcg caaatcatga tgtgaccagc
     1741 cttataaaac ttcacgacac gctccacgag aaaatcagga tgctgaacgg actttcaaaa
     1801 gatcttgcgg agcggacgaa cgaggatccg ccttttgaag cgatcctgga gaaagaatct
     1861 cattccaacc tgaacggcct gatggatgtc cttatcgaac aggcctcatc ggaattttcc
     1921 gttgctcctc cccagatgag cccggaagag agaattcgat ttgtgagcat gctcaaaacg
     1981 cgccacctct tctcgatgaa aggggcggtg gcaaaggtgg cggaacgtct cggcgtttct
     2041 gaagcgacgg tctatcggta tctgaagaaa gcgtgagcat gctctttatt gaaggctgtg
     2101 ttaaagatcg ttttggagta gcgtctctcg cccagaattc acacgttgca aaagaaggaa
     2161 aggtgtgata aaaatttatt atcacaccgt cgcttcttct tcgtgaagaa ggcggtctgt
     2221 tcggaaggag tggagcgaag aagatgagag aactgatgac cggaaacgag gccattgcca
     2281 gaggggcctg ggaagcgggg tgtcacgtgg cagccgccta tccggggacc ccgagcacgg
     2341 aaatcctcga aaatgtctcc cgctacaagg agatctattc cgagtggtcc acaaacgaaa
     2401 aagtttctct ggaggtcgtc tcgggagcgt caatggctgg ggcccgggcg atgtgcgcca
     2461 tgaagcacgt gggattgaac gtggcggcgg atcctttttt cacgatgtcg tatatcggcg
     2521 tcaacggagg tctcgtcatc gtcgacgcgg acgaccccgg acttttcagc tcgcagaacg
     2581 aacaggacaa ccgctggtat gccctccatg caaagctccc catgctcgaa ccttcggaca
     2641 gccaggaatg tcttgatttc atgaaggccg cctttgatat ctctgaacgc ttcgacactc
     2701 cggttctcct tcgcgtcacg acgcgtatct gccacagcaa gagcgtcgtc gagacaggcg
     2761 aacgtatgga acgactcatt cgtgagtata cccgcaatcc ggcgaagaat gcgatgcttc
     2821 ctgtctttgc gaaggcgaag cactatctcg tcgaaaaacg tcttcttgat ctccgtgagt
     2881 ttaacgagac gtcgccgttc atctcgtacg tggagggcga taatgaaata ggcgtactca
     2941 cgagcggcat cggttatcaa cattcgaggg aggcgcttgg cgacagagtg agctatctga
     3001 aactgggtat gacctggccc ctgcctgaga aagccatccg ggattttgct tcgaaagtga
     3061 agaatctttg gattgttgag gagaacgacg cattcatcga gggggcagta aggggcatgg
     3121 gtatcgcctg ttccgggaag gatgcgctcc ctcttgtgga tgaactctct ccggctctga
     3181 tagcgaaagc aattctcgga acgaagccct cctccggacc cgataccgga attgcgccgc
     3241 caaatcgtcc tccgctcctc tgcgccggat gcccgcaccg ggggcttctc tttgcgatga
     3301 gcaagaagaa gggtttggtt gtgatgggag atataggctg ctatggactt gggtcgatgc
     3361 ctccgctcaa cgtcggcgag agcaccattt gcatgggagc ggggttttca gcagtcgtcg
     3421 gtttccagaa agcgcaggaa aaggccggcc gctcccagaa agtcttcgga atcctcggtg
     3481 attccacttt tttccattcc ggtattacgg gccttatcga cgctgtggtg aacaagtcga
     3541 gg
//
LOCUS       DAOTIL010000189         3541 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminobacteriaceae bacterium isolate AMR_MDS_3026
            NODE_31306_length_3541_cov_3.059954, whole genome shotgun sequence.
ACCESSION   DAOTIL010000189 DAOTIL010000000
VERSION     DAOTIL010000189.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868050
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminobacteriaceae bacterium (wastewater metagenome)
  ORGANISM  Aminobacteriaceae bacterium
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae.
REFERENCE   1  (bases 1 to 3541)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3541)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:19:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,224
            CDSs (total)                      :: 3,198
            Genes (coding)                    :: 3,184
            CDSs (with protein)               :: 3,184
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3541
                     /organism="Aminobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_31306_length_3541_cov_3.059954"
                     /isolate="AMR_MDS_3026"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:3034609"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1159
                     /locus_tag="P5201_05125"
     CDS             <1..1159
                     /locus_tag="P5201_05125"
                     /inference="COORDINATES: protein motif:HMM:NF013884.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="acyltransferase family protein"
                     /protein_id="HRV97949.1"
                     /translation="LCRTMRIQFIKKKARWDFMQQQHAERVYFFDAYRTIIVLSVLAL
                     HAAMSYMAYVPGWWYVINPEKSLFFLFWVLIVNTGDMPAIFFLAGYFAPRSLEKRGRM
                     LFLKEKALHIGIPWIVGVLAFAPLFAMATWRSLNLPLPETYMEFVRTIWLGPGYQQSH
                     FWFLGVLMVFLIVFAAVGSPRAASGRSPLFWLAGWWGLSSAAFFLSSLYLHPDLWIRI
                     SHIYFQPARIVSYALAFYLGARAWRDGWFDGPRPGFGAIALSGLATVAGSWSVIFLAM
                     NFPVKETLALKALHGMSHSFASLSIAVFFLFLCRALFDRPSPVWRTLSEASYGIYWLH
                     QMILMPAVYLLIPWNLPIGIKFILALGGTYILCAFLTIHGLKKLPVLRRLF"
     gene            complement(1363..2076)
                     /locus_tag="P5201_05130"
     CDS             complement(1363..2076)
                     /locus_tag="P5201_05130"
                     /inference="COORDINATES: protein motif:HMM:NF015448.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004826 - phenylalanine-tRNA ligase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase beta subunit-related
                     protein"
                     /protein_id="HRV97950.1"
                     /translation="MKEIYYSITNDVFTPFPDYVRGVVVARGVKNGPSPAELTALLRE
                     AEEQLRGKLGSGNIAEHPRIASWREAFRKTGVKPAEFRSSIEAMARRVAKGQELPSIN
                     ALVDIGNVLSLRHLVPTGGHALDDVRGDIVLRAATGEETFVPFGETETEHPLPGEIIF
                     AEGDVVMTRRWSWRQGVHTLTLPESTSIEFNVDGLPPVTSGEVESVCGEVVELVSRFC
                     GGTFETGLLTAASPRMRLL"
     gene            complement(2089..>3541)
                     /locus_tag="P5201_05135"
     CDS             complement(2089..>3541)
                     /locus_tag="P5201_05135"
                     /inference="COORDINATES: protein motif:HMM:TIGR01891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="M20 family metallopeptidase"
                     /protein_id="HRV97951.1"
                     /translation="MRKAELGSFIEKKRDVFIELSDKIWAFAETAFVEFQSADLLCEA
                     LEKEGFSVERGTGGVATAFCGSWGSGSPVIAFLGEYDALSGLSQKGGVALKEPLVPGG
                     NGHGCGHNNLGAGAFAAAVAFREYLKSSGKSGTVRYYGCPGEEGGSGKAFMARAGVFD
                     DVDAALTWHPGSVNAVFPFSSLANFQVAYRFKGKSAHAAACPHLGRSALDAVELMNVG
                     VNYLREHVIPEARMHYAITDSGGFSPNVVQPKAEALYLLRAPRTPQVEEIYERVNKIA
                     RGAALMTETELEIRFIKACANVVQNNVLERVLARNFAELGVPEYTEEEISFARAIFDG
                     IAPSERSSDLDQYSAPGGAAGKEAAKRLRERPIADGVLPYGESDFVLPGSTDVGDVSW
                     IVPTCQAWVATWPNHTPGHSWQIVSSGVTPLAHKGMLHAGKVLAAAAADLFDDPSLID
                     AAKKELRERLDGVSYRCAIPDEVVPKAIQPSGR"
BASE COUNT          549 a         1226 c         1096 g          670 t
ORIGIN      
        1 gttgtgcaga acaatgagga tacagttcat caaaaaaaaa gcgaggtggg atttcatgca
       61 acaacagcac gcggagcggg tctacttctt cgacgcctac cggacgatca tcgtcctctc
      121 cgtcctggcg cttcacgcgg cgatgagcta catggcctat gttccggggt ggtggtacgt
      181 catcaacccc gagaagagcc tcttcttcct cttctgggtg ctcatcgtga acacgggcga
      241 catgcccgcg atcttcttcc tggcggggta cttcgccccc cgttcgctcg aaaagcgggg
      301 gcggatgctc tttctgaagg aaaaagcgct ccacatcggc attccatgga ttgtcggcgt
      361 cctcgcgttc gcgccgcttt tcgccatggc gacgtggcgc tcgctgaatc tgccgctgcc
      421 ggagacgtac atggagttcg tccggacgat atggctcggc cccggctacc agcagtcgca
      481 cttctggttc ctcggcgtgc tcatggtctt tctgatcgtc ttcgccgccg tcggctcccc
      541 gcgcgccgcc tccgggcgct cgcccctctt ctggcttgcg gggtggtggg gcctctcctc
      601 cgccgcgttc ttcctttcgt ccctctactt gcacccggat ctctggatac gaatcagcca
      661 catctacttc cagcccgccc ggatcgtctc ctacgccctc gccttctacc tgggagcccg
      721 ggcgtggcgc gacggctggt tcgacggccc ccggcccggc ttcggcgcga tcgcgctctc
      781 ggggctggcc acggtcgcgg ggtcgtggtc ggtgatcttc ctcgccatga acttccccgt
      841 gaaagagacg cttgccctca aggcgctgca cggcatgagc cactccttcg cctccctttc
      901 catcgcggtc ttcttcctct tcctctgccg ggcgctcttc gaccgtccgt ctccggtgtg
      961 gaggacgctg agcgaggcgt cgtacggcat ctactggctc caccagatga tcctgatgcc
     1021 cgccgtctac ctgctgattc cgtggaacct cccgatcggc atcaagttca tcctcgccct
     1081 cgggggcacg tacattctgt gcgcgttcct cacgatccac ggactgaaga agctcccggt
     1141 gctacggcgg ctcttctgac gctcccgtgc agggacaaag gacgcgcggc tcccgagttt
     1201 gcatgggggc tgcgcgtttg tgttttcgga gaatccgtaa ctgttatagc ggccagaatc
     1261 ataccgcatt atcccgctgc gaggacgcga gcgatcgaca gcgcaaaacc tccggcacgc
     1321 ttccgccgga caaacgagga aaactcccgt ttactcgcct gcttacagca ggcgcatccg
     1381 gggagaggcc gccgtgagca gtcccgtctc gaacgtgccg ccgcagaagc gcgacacaag
     1441 ctcgacgacc tcgccgcaga cggactccac ctcgccggac gtcaccggcg gcagtccgtc
     1501 cacgttgaac tcgatggagg tcgactcggg cagcgtgagg gtgtgcaccc cctgccgcca
     1561 gctccaccgc cgggtcatga ccacatctcc ctccgcgaag ataatctcgc cgggaagcgg
     1621 gtgttcggtc tccgtctcac cgaagggaac aaatgtctcc tctccagtgg cggcgcggag
     1681 gacgatgtca ccccgcacgt cgtccagcgc gtgcccgccc gtcgggacga gatggcggag
     1741 cgagaggacg ttgccgatat ccacgagggc gttgatcgac ggaagctcct gccccttcgc
     1801 gacgcggcgc gccatggcct cgatggagga acggaactcg gcgggcttca cgcccgtctt
     1861 ccggaaggcc tcccgccacg acgcgatgcg cgggtgctcc gcgatgttac cggagccgag
     1921 ctttccgcgc agctgctctt cggcctcgcg gagcagcgct gtaagctccg ccggagaggg
     1981 accgttcttc acgccgcgcg cgaccacgac cccccggacg taatcgggga acggggtgaa
     2041 gacgtcgttc gtaatgctgt agtaaatttc tttcatgcgt tgttcctcct atctgcccga
     2101 tggctggatc gccttcggga cgacctcgtc ggggatggcg cagcgatacg aaacgccgtc
     2161 gaggcgctcg cggagctcct tcttcgcagc gtcgatcagc gatgggtcgt cgaacaggtc
     2221 ggcggccgcc gccgcgagga ccttccccgc gtggagcatc cccttgtgtg caagcggggt
     2281 gacgcccgag gagacgatct gccacgagtg ccccggagtg tggttcggcc acgtcgccac
     2341 ccaggcctgg caggtgggaa cgatccagct cacgtcgccc acgtcggtcg atccggggag
     2401 gacgaagtcg ctctctccgt agggaagcac cccgtcggcg atcgggcgct cccggaggcg
     2461 tttcgccgcc tccttgcccg ccgccccccc cggcgcggag tactggtcga ggtcggagct
     2521 gcgctcggag ggcgcgatcc cgtcgaagat cgcccgggcg aaggagatct cctcctccgt
     2581 gtactcgggc acgccgagct cggcgaagtt ccgcgcgagt acgcgttcga gcacattgtt
     2641 ctgcaccacg ttggcgcacg ccttgatgaa gcggatctca agctccgtct ccgtcatcag
     2701 ggccgcgccg cgggcgatct tgttcacccg ctcgtagatc tcctcaacct gcggcgtgcg
     2761 cggggcgcgg agcaggtaaa gcgcctcggc cttcggctgc accacgttgg gggagaagcc
     2821 cccggagtcg gtgatggcgt agtgcatccg cgcctcgggg atgacgtgct cgcgcaggta
     2881 gttcacaccc acgttcatca gctccaccgc gtcgagcgcg ctccgcccga ggtgcgggca
     2941 ggcggcggcg tgggcgctct tccccttgaa ccggtaggcg acctggaagt tggccagcga
     3001 gctgaagggg aagaccgcgt tcaccgagcc ggggtgccac gtgagcgcgg cgtccacgtc
     3061 gtcgaagacg cccgccctcg ccatgaaggc cttcccggag ccgccctcct cgccggggca
     3121 gccgtagtag cgcaccgtgc cgctctttcc gctgctcttc aggtactccc tgaaggcgac
     3181 ggccgcggcg aaggcccccg cgcccaggtt gttgtggccg catccgtggc cgttcccgcc
     3241 ggggaccagc ggctccttca atgcgacgcc gcccttctgg ctcagcccgg agagcgcgtc
     3301 gtactcgccc aaaaaggcga tcaccggcga accgctcccc cacgagccgc agaaagcagt
     3361 cgccacgccg ccggttccgc gctccacgga gaacccctcc ttttcgaggg cttcgcagag
     3421 taaatccgcg gactgaaatt ccacgaacgc cgtctcggcg aacgcccata tcttgtcgct
     3481 cagttcgatg aatacgtccc gttttttctc gatgaaactc ccgagttcag cctttcgcat
     3541 t
//
LOCUS       DAOTIL010000190         3535 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminobacteriaceae bacterium isolate AMR_MDS_3026
            NODE_31360_length_3535_cov_4.593966, whole genome shotgun sequence.
ACCESSION   DAOTIL010000190 DAOTIL010000000
VERSION     DAOTIL010000190.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868050
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminobacteriaceae bacterium (wastewater metagenome)
  ORGANISM  Aminobacteriaceae bacterium
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae.
REFERENCE   1  (bases 1 to 3535)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3535)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:19:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,224
            CDSs (total)                      :: 3,198
            Genes (coding)                    :: 3,184
            CDSs (with protein)               :: 3,184
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3535
                     /organism="Aminobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_31360_length_3535_cov_4.593966"
                     /isolate="AMR_MDS_3026"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:3034609"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2833)
                     /locus_tag="P5201_05140"
     CDS             complement(<1..2833)
                     /locus_tag="P5201_05140"
                     /inference="COORDINATES: protein motif:HMM:NF024867.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HRV97952.1"
                     /translation="MKISEFFVQNFGIFSGSGTSLSKGLTIFHGENESGKTTLMNFFR
                     RILFPREKYSRTRGNIYEPLSGARHGGSARVRMEDGREFVLTLDGKKNFVLPASGGSA
                     APLPSNFFSIGKDVYESVFAMGLNDMQSMESLNKADIAARFFSAGSGLGSASLPRLLS
                     SLEERGNELYRPGLAKGASAVNRLLASMKETDERIRELRMASGSWGERRDALDSAERA
                     LEQRREELAELQRRLSFLELLEKGLAPRAALGEIERSLGELSALPPFPEGGLDKLERL
                     KDERKRLEASCVRLESEIRAKEEEAAAPASDPLLLCTEYETEIRALEQEGEQLRAALA
                     RKNLLERELLASRRSFLRNIEDLCPWWTEEHLMSADISADAIAFARRTAERKEVLERK
                     RGEGEKSLAQWNRLREDRRSEAASLEKEMGVVEKRAKRASERWGLITRLRSVFTELCS
                     QEDELNSLEEARSTLLAERDRALEQEPIPPGIMVVLISAFLLLVGAGAALQAFLASDY
                     LWAFGSATLFMASLLAWFAHKDLSKKYATARGQWVKRLEDLDSRLEETTLLVESQKTR
                     VDRLRTQREELGSSLGINAPRFESAMEHLLAEGEGDNSAQERYTFLSERNRQMATVLA
                     RMDAEGEAMEQEVARTVKELETLNEAWSRWLTEHRFDRNLAPRDMEGIVPRILQLRSE
                     EASLAARAAEGRELDDAVEAIRKRIQELAGRLRPSFEALGEPAPSAADPASIRAFSTL
                     LRRAAEKRGEIAACERDAAMLRQNLDDARAEASETERTLNDLFAAAQTDGEDAFRALA
                     EAWRKRDALLAGRLQERKVLLGLFGSDEALRAAEEEYAFRSPEDVRSETESLRERAER
                     ARSELDAMVDAKGRISSELERMGTDERLGEQLFARKGMERKLDEHLEEWLSNLLAHHF
                     LEVSRARHERERQPEVIRRAGEYLALMTGD"
     gene            complement(2830..>3535)
                     /locus_tag="P5201_05145"
     CDS             complement(2830..>3535)
                     /locus_tag="P5201_05145"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRV97953.1"
                     /translation="HSAEILCHDPLVVYPGNIQGRHIGETGKKGVFSVAMGSAGGHPG
                     GRGGVEFIPCDIVRWRREALSIEGMQRDEELFAAFDRLRDGVRNDAGGRPVIVRASVG
                     GRGKLSALLRRPGFLSGPGGLVESLNQGEEGRSDFVYIESVVDDTASPFDLEALSSGN
                     HFVGDFLKEVSAFERRGNLREGLMEILSARGVREKLPREVFDRVDALPDDEILSLLRR
                     GTTLALAGLLEGEDDE"
BASE COUNT          557 a         1267 c         1084 g          627 t
ORIGIN      
        1 ggtcgccggt catcagggcg aggtactcgc ccgcccggcg tatgacctcc ggctgccgtt
       61 cgcgctcgtg acgcgctctg gagacttcca ggaagtgatg cgccaggagg ttcgagagcc
      121 actcttcaag gtgctcgtcg agcttccgct ccatcccttt tctggcgaag agctgttcac
      181 ccagccgttc gtcggtcccc atcctctcca gctcggagga gatgcgcccc ttggcgtcga
      241 ccatggcgtc gagttcggag cgggcgcgtt ccgcgcgttc gcggagcgac tccgtctccg
      301 acctgacatc ttccggagaa cggaacgcat actcctcctc ggcagcccga agcgcctcgt
      361 ccgaaccaaa gagacccagg agcaccttcc gctcctggag ccgcccggcg agcagcgcgt
      421 cgcgcttccg ccacgcctcg gcgagggcgc ggaacgcatc ctcgccgtcg gtctgcgccg
      481 ccgcgaagag gtcgttcagc gtgcgctccg tctcgctcgc ctccgctctc gcgtcgtcca
      541 gattctggcg gagcatcgcg gcgtcccgct cgcacgcggc gatctcgccg cgcttttctg
      601 ccgcccgccg caaaagcgtc gagaaggcgc ggatcgacgc gggatctgcc gccgagggcg
      661 caggttcgcc aagggcctcg aaggagggcc tgagacgtcc tgcgagctcc tgaatccgtt
      721 tccggatggc ttcgacggcg tcgtccagct ccctcccctc ggccgcccgc gcggcaagag
      781 aggcctcctc cgagcgcagc tgaagaatcc tcgggacgat cccctccatg tcgcgggggg
      841 caaggttacg gtcgaacctg tgttcggtca gccagcggct ccacgcctcg ttcagcgtct
      901 ccagctcctt gacggtacgc gcgacctcct gttccatggc ctcgccttcg gcgtccatgc
      961 gcgcaagaac ggtcgccatc tgtctgttcc gttcgctgag gaaggtgtag cgctcctgcg
     1021 cgctgttgtc cccctcgccc tcggcgagga ggtgctccat cgcgctctcg aagcggggcg
     1081 cgttgatgcc gagactgctc cccagctctt ccctctgcgt ccgcaggcga tcgaccctcg
     1141 tcttctggct ctcgacgagc aaggtcgtct cctcgagcct ggagtcgaga tcctccagcc
     1201 gtttcaccca ctgccccctg gcggtcgcat atttcttcga cagatccttg tgcgcgaacc
     1261 acgccagcag ggaggccatg aagagcgtcg ccgagccgaa ggcccacagg tagtccgacg
     1321 ccaggaaggc ctggagcgcg gcgcccgcgc cgaccagcag gaggaatgcg gagatcagga
     1381 cgaccatgat tcccggcgga atcggctcct gttcgagggc gcggtcccgc tccgccaaga
     1441 gcgtcgaacg cgcctcctca agggagttca gttcgtcctc ctggctgcag agctcggtaa
     1501 agacgctccg gaggcgcgtg atgagccccc agcgttcgga ggcccgcttc gcccgcttct
     1561 cgacgacgcc catctccttt tccagcgagg cagcctcgct tctgcggtcc tcgcgaaggc
     1621 ggttccactg ggcgagcgac ttctctccct cgccccgctt ccgttcgagc acctccttgc
     1681 gctccgcggt cctgcgggcg aaggcgatcg catccgcgga gatgtcagcg ctcatcaggt
     1741 gctcctccgt ccaccacgga cagagatcct cgatattgcg gaggaagctc ctgcgcgagg
     1801 ccagcagttc gcgctccagc agattcttgc gtgcaagcgc ggcgcgcagc tgctccccct
     1861 cctgctccag cgcgcggatt tccgtttcgt actccgtgca gagaagcaac gggtcggaag
     1921 ccggcgccgc ggcctcctcc tccttcgcgc gaatctcgga ctcaaggcgg acacaggagg
     1981 cttcaagccg cttccgttcg tctttcaagc gctccagctt gtcaagccct ccctcaggga
     2041 acggaggaag ggcggagagt tcgccgaggg atcgctcgat ctcgccgagc gcggcgcggg
     2101 gcgccagccc cttctccaga agctccagaa aggagagccg ccgttgcagt tccgcaagtt
     2161 cctcgcgccg ctgttccagc gcgcgctccg cgctgtcgag cgcatcgcgg cgctcacccc
     2221 acgaaccgct cgccatgcga agctcgcgga tccgctcgtc cgtctccttc atcgacgcca
     2281 gcaggcggtt cacggccgag gcgcccttgg cgagtccggg ccggtagagc tcgttccccc
     2341 gctcctccaa tgaggagagc agcctgggaa gcgatgccga acccaggccc gaaccggcgg
     2401 agaagaagcg cgccgcgatg tcggccttgt tcagcgattc catcgactgc atgtcgttca
     2461 gccccatcgc gaagacgctc tcgtagacgt ccttgccgat ggagaagaag ttcgacggaa
     2521 gcggcgcggc gcttcctccc gacgccggaa ggacgaagtt cttcttcccg tcgagcgtga
     2581 ggacgaactc cctgccgtcc tccatgcgga ccctggcgga accgccgtgc cgcgcgccgc
     2641 tcagcggctc gtagatgttc cctcgggttc gcgagtactt ctccctcggg aaaagaatcc
     2701 gccggaagaa gttcatcagc gtcgtctttc cgctctcgtt ctcgccgtga aagatcgtca
     2761 gccccttgga gagagaggtt cccgacccgg aaaaaatacc gaagttctgt acgaaaaact
     2821 cgctgatctt cattcgtcgt cctccccctc aagcagtccc gcaagcgcaa gcgtcgtccc
     2881 tctgcgcagc agcgagagga tctcgtcgtc ggggagcgca tccaccctgt cgaacacctc
     2941 gcgggggagc ttctccctga cgcccctcgc ggagagtatc tccatcaacc cttccctgag
     3001 attgccgcgg cgctcgaagg ccgaaacttc cttgaggaag tcgccgacga aatggttccc
     3061 cgaggacagc gcttcgaggt cgaacgggga cgcggtgtcg tcgacgacgc tctcgatgta
     3121 gacgaagtcg ctccgcccct cctcgccctg attcaggctc tccacgagcc cgccgggacc
     3181 ggagaggaac ccgggacggc gcagaagcgc gctcagcttg ccgcgtccgc cgaccgacgc
     3241 ccggacgatc acagggcgtc cgcccgcatc gttccgtact ccgtccctga gacggtcgaa
     3301 ggcggcgaag agctcctcgt cacgctgcat tccttcgatg gagagggctt ccctccgcca
     3361 gcggacgatg tcgcacggga tgaactccac gccgcctctc ccgccgggat gcccgcccgc
     3421 ggagcccatg gcgaccgaga agacgccttt cttgcccgtc tcgccgatat gccgcccctg
     3481 aatgttgccg gggtagacga ccagtgggtc gtggcagaga atctccgctg agtgc
//
LOCUS       DAOTIL010000191         3514 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminobacteriaceae bacterium isolate AMR_MDS_3026
            NODE_31589_length_3514_cov_4.102053, whole genome shotgun sequence.
ACCESSION   DAOTIL010000191 DAOTIL010000000
VERSION     DAOTIL010000191.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868050
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminobacteriaceae bacterium (wastewater metagenome)
  ORGANISM  Aminobacteriaceae bacterium
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae.
REFERENCE   1  (bases 1 to 3514)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3514)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:19:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,224
            CDSs (total)                      :: 3,198
            Genes (coding)                    :: 3,184
            CDSs (with protein)               :: 3,184
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3514
                     /organism="Aminobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_31589_length_3514_cov_4.102053"
                     /isolate="AMR_MDS_3026"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:3034609"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1189)
                     /gene="cls"
                     /locus_tag="P5201_05150"
     CDS             complement(<1..1189)
                     /gene="cls"
                     /locus_tag="P5201_05150"
                     /inference="COORDINATES: protein motif:HMM:TIGR04265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008808 - cardiolipin synthase activity
                     [Evidence IEA];
                     GO_process: GO:0032049 - cardiolipin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin synthase"
                     /protein_id="HRV97954.1"
                     /translation="MNFLKEYSFSFLWAYGIFIAGIIFLEERNPDRTIAWLLVLLLLP
                     VIGFLIYFFFGPDLRERENFRKIKKKRRKNRKGPLFVSHEHNEPCAFLGEHTKKTASL
                     LTKSAKARVSTHNSVEMLVNGEATFASIKHALRQAKRYIHLEYYSIDNDAIGGEIRDI
                     LLEKAAEGVDVRLIYDSVGSWKLGKKFIDTLKHGGVHVHSFLSVSFPMLRRDLNFRNH
                     RKIIVIDGITGYLGGLNIGDSYIHGEPGLGIWRDTHLKIEGEAVACLDEIFAADWTFC
                     DLKAPPAHDIPNAVHNEITPVQIVSSGPDSNWKSILHGYFSMISNARESLWLCTPYLI
                     PGKTLSTALTVASMSGVDVRVIIPHAADHAMVHWASLSSVEELLRAGVKVYSYRKGFH
                     HAKI"
     gene            1480..1563
                     /locus_tag="P5201_05155"
     tRNA            1480..1563
                     /locus_tag="P5201_05155"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1513..1515,aa:Leu,seq:tag)
     gene            complement(1568..2134)
                     /locus_tag="P5201_05160"
     CDS             complement(1568..2134)
                     /locus_tag="P5201_05160"
                     /inference="COORDINATES: protein motif:HMM:NF013077.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HRV97955.1"
                     /translation="MNLVLETIKKRRSVRSFRPDQITDDELNSIIESGLWAPSGHNTQ
                     PWHFLVVQDKSKIDQMSRSTIEKMANSPIDWVRKLAAKEGYHLFHGAPTVIIVSGRQS
                     GDTFLPVVADCAAAIQNMLLAAESLNIGTCWIGLTSFLFEDSEETASLKIPEGYAPYY
                     SIAVGYKSAGFIPAPPTRKEGTVSTFGK"
     gene            complement(2148..2726)
                     /locus_tag="P5201_05165"
     CDS             complement(2148..2726)
                     /locus_tag="P5201_05165"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRV97956.1"
                     /translation="MNADAYVVLGLKELFRRTDWKCFSYPGTICFPVDLHQPIVPLAD
                     GIGFNGGFLHKNGGDQKRVNLIMLGVFFYQSGVTGGICCGKTRIHSSEVAFDRVRSFS
                     LIPRGPAAQHRMVFSARRERRCKGWLRYKTGEKDQGYQETKNGIISDSHSVAAPFCRE
                     KTAAEGEKENAQWHFPHFWDIVAYTHRQKHFF"
     gene            2901..>3514
                     /locus_tag="P5201_05170"
     CDS             2901..>3514
                     /locus_tag="P5201_05170"
                     /inference="COORDINATES: protein motif:HMM:NF024214.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase family protein"
                     /protein_id="HRV97957.1"
                     /translation="MEVAAVVLAAGYSSRMGFFKPLALLNGVSALERILRSMRGAGVR
                     NVLVVTGFNEDVVASAAQRAGARAVYNPRYAEGMFTSVQVGVAALPHSADAFFLLPVD
                     IPTVRVSTLRLMMESASSNGILHPLFRGKRGHPPLIAAALAPHILDFSGEGGLRAFFD
                     GWATWNQDFDVADEGVLMDMDTPQDFEKIHRRLSGLRVPSKMER"
BASE COUNT          809 a          861 c          913 g          931 t
ORIGIN      
        1 ttatctttgc gtgatggaaa ccttttctgt agctatacac tttgactccc gctctcaaaa
       61 gttcttctac ggaagaaaga gatgcccagt gcaccatcgc gtggtcggcg gcgtgaggaa
      121 tgattacccg tacatcgacg ccgctcatgg atgcgactgt cagagcggtc gaaagggttt
      181 ttcccgggat aaggtacggg gtgcagagcc aaagagactc tcttgcgttg gatatcatgg
      241 agaagtagcc gtgcagaata gatttccagt ttgaatcagg tccgctggaa acgatctgca
      301 caggagtgat ctcgttgtgg acagcgttcg gtatgtcatg cgccggaggg gcttttaaat
      361 cgcagaaagt ccagtctgcc gcgaaaattt catcaagaca tgctaccgct tcaccttcaa
      421 tcttcagatg cgtatcccgc cagattccca gtcccggctc cccgtgaata tacgaatcgc
      481 ctatgttcaa gcctcctaga tatccggtga tgccgtcgat gacaatgatc ttcctgtggt
      541 ttcggaaatt gagatcgcgg cgaagcatgg ggaaggagac tgaaagaaaa gaatgaacat
      601 ggacaccgcc gtgtttgagg gtatcaatga attttttccc cagcttccag ctgccgacac
      661 tgtcataaat caggcgcaca tcgacgcctt cagcagcttt ttccaaaagg atgtctctga
      721 tttctccgcc gattgcgtcg ttgtcgatag agtaatactc aaggtgaatg tatcttttcg
      781 cctgccggag cgcgtgtttt atagaggcga aggtggcttc gccgttcacc agcatttcta
      841 ctgagttatg agtcgaaacc cgcgccttgg cgctttttgt cagcaaggat gccgtttttt
      901 tcgtgtgttc ccctaagaag gcacagggtt cgttgtgttc atgagagacg aagagggggc
      961 ctttcctgtt ttttcttcgt ttctttttta tttttcggaa attttctcgt tctctgaggt
     1021 ccggaccgaa gaaaaaatag atgagaaatc cgatgaccgg cagcaggagg aggacgagca
     1081 gccaggcgat ggtcctgtcg ggattgcgct cttcgagaaa gataatgccc gcaataaaga
     1141 tgccgtatgc ccagagaaaa ctaaaggaat actctttgag gaaattcatt accttctctg
     1201 gaatgcggtg aggcgcttgg cgccagaaat caccgtcaag gagcagtctg atattgtctc
     1261 cgatcgactc cgcatttgag aggatgaaga gaaacgtcgc aagtccgaaa agcagccaga
     1321 agaatgtcga tgtacgccgt atttttttca gagccgtctc ctccctcttt tccactcagg
     1381 tttgaaagtg tgaatgaata aaacattcgt ttacgctatt cgttttttag cagggcttca
     1441 caacgcccct ttatgatggt attattccac ggcatgcgtg cgagagtggt ggaacggcag
     1501 acacgctgga cttaggatcc agtgcctttg ggcgtggggg ttcaagtccc ccctctcgca
     1561 ccatttttta tttgccgaac gtgcttactg ttccctcttt ccgggtagga ggagcgggaa
     1621 tgaatccggc gcttttgtat cccactgcaa tggagtagta cggagcatag ccttccggta
     1681 tctttaaaga agccgtttct tcagaatcct cgaagagaaa actcgtcagg cctatccaac
     1741 aggttcctat gttcagggat tctgcggcca gcaacatgtt ctgaatagct gctgcgcagt
     1801 ccgcgacgac aggaaggaat gtatctcccg actgccttcc ggaaacgatg atgaccgtag
     1861 gcgcgccatg gaacagatgg tatccttctt tcgcagcaag cttccgcacc caatcaatcg
     1921 gcgagtttgc catcttttcg atcgtggaac ggctcatctg gtcaattttt gatttgtcct
     1981 gcacaacaag aaagtgccac ggttgggtgt tgtgtccgct tggggcccat aacccgcttt
     2041 cgataattga attcagctcg tcgtccgtaa tttggtccgg tcgaaaactt cgtacgctac
     2101 ggcgtttctt tattgtctcc aaaacaaggt tcattgtaat ttcctcctta aaagaaatgt
     2161 ttttgtcgat gtgtatatgc tacaatatcc caaaaatgcg gaaaatgcca ctgtgcattt
     2221 tctttttcac cctctgcggc agttttttct ctgcagaaag gagcggcaac actatgagaa
     2281 tccgaaataa taccgttttt tgtttcctga tatccttggt ctttttctcc tgttttgtag
     2341 cgcaaccagc ccttgcagcg cctttctcga cgagccgaga ataccatgcg atgctgagcg
     2401 gcaggacctc tcgggatcaa agaaaaagaa cgcacgcgat cgaaagctac ttcagaagag
     2461 tgaatcctcg tctttccgca gcaaatgcct cccgttacgc cggattgata gaaaaatact
     2521 cccagcatta taaggttgac ccgtttctgg tcgccgccat tcttgtgaag gaatccaccg
     2581 ttaaatccaa tgccgtcagc aaggggaact atgggttgat gcaggtcaac tggaaagcaa
     2641 atggtccctg gatacgaaaa acatttccag tccgtacgac gaaacagctc cttgagcccg
     2701 agaacaacat acgcatcggc gttcatatac tggcatcgaa tatccagcgt gccggcggag
     2761 atgtggataa ggggctcgac aggtatcggg gccgctcgct ggcctcctat agaaattcgg
     2821 tcctgcggaa ctattcggcg atctacacct tgtttgctcg ccgttgagtg tgtagcgaac
     2881 acaacaaagg agatctgtcg atggaagtcg cagcggttgt tctcgcggcg gggtattcca
     2941 gccgtatggg ctttttcaag ccgcttgccc tcctgaacgg agtgtctgct ctggagcgga
     3001 ttcttcgttc aatgcgtggc gccggggtac ggaatgttct cgtcgtgaca ggatttaatg
     3061 aagacgttgt cgcctcagct gctcaacgcg ccggcgcccg ggccgtctat aatccccgtt
     3121 atgcggaggg tatgtttact tcagtacagg taggcgtagc tgcgcttccg cacagtgcag
     3181 acgctttttt cctcctgccc gtagatattc cgacagttcg ggtttccaca ttgcgtttaa
     3241 tgatggaaag cgcttcttcc aatgggattc ttcaccctct gtttcgaggg aagcggggac
     3301 atcctccgct tatcgcggct gccttggcgc cccatatact tgatttttcc ggagaaggcg
     3361 ggttgcgcgc cttttttgat gggtgggcaa cgtggaatca agacttcgac gtagcggacg
     3421 agggcgtgct catggatatg gatacgccgc aagactttga aaaaattcac cgtcgtttga
     3481 gcggactgcg cgttccctcg aaaatggaac gaga
//
LOCUS       DAOTIL010000192         3491 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminobacteriaceae bacterium isolate AMR_MDS_3026
            NODE_31863_length_3491_cov_3.963912, whole genome shotgun sequence.
ACCESSION   DAOTIL010000192 DAOTIL010000000
VERSION     DAOTIL010000192.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868050
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminobacteriaceae bacterium (wastewater metagenome)
  ORGANISM  Aminobacteriaceae bacterium
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae.
REFERENCE   1  (bases 1 to 3491)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3491)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:19:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,224
            CDSs (total)                      :: 3,198
            Genes (coding)                    :: 3,184
            CDSs (with protein)               :: 3,184
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3491
                     /organism="Aminobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_31863_length_3491_cov_3.963912"
                     /isolate="AMR_MDS_3026"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:3034609"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..809)
                     /locus_tag="P5201_05175"
     CDS             complement(<1..809)
                     /locus_tag="P5201_05175"
                     /inference="COORDINATES: protein motif:HMM:NF015983.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HRV97958.1"
                     /translation="MYCTLKERYRLRGWEQLPYAIQDVASGGVVFLDEKAFQAASFCN
                     GMVDVASPLLLPAHRRAVSLLKEQGMVEECAFGEGLKEEQKYRLIPCRYMRKAHWSIT
                     GRCNLKCRHCFMSAPHAKYGELLLEQCLNIVRQISEAGILDVSLTGGEPLVRNDFFDI
                     VDALLDERIMISQVYTNGVLVDEALLNKFEARGIRPEFLLSFDGIGCHDWLRGVPGTE
                     RQTIDAIRLLRSRNFTVSIETALYRGNLHTLPTSLELFAGLGVESWKATPAT"
     gene            1195..2439
                     /locus_tag="P5201_05180"
     CDS             1195..2439
                     /locus_tag="P5201_05180"
                     /inference="COORDINATES: protein motif:HMM:NF014385.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HRV97959.1"
                     /translation="MKKRPLLYVVFLLPLILPCAAFSAHMDEMQYLALVMERNNDLAA
                     ARQEIEARLFSARAELAIQRPSLSLSAETSKWLDKNRGDQQADLSLSHRVNLSGAYGL
                     QERDLLLGVEILRNQYADAVNERLALAASAYRQAVMAALNREAMEQIVEKRRESLTIT
                     KEKFDKELVSLLELLRAQSQVDDGEAFLLQARQKYEQQLVEMSALAGGAPAIPSVALP
                     ELGGMNLVVDEEKAWDNRPDVAALLLSKERASVQRSLAAKGLSPFVDLSVGLRVLEDH
                     IFPQERGGEVFVRAVLTVPLADGGKTSNSTEAATLMLRKAEYELAARRDSLKREADMV
                     RERWNRALEIVAIRRRQAERADKELEIAELMYREGMGSQLDLINAQEADQKSRTEFLS
                     AVQELWLVLAEADRVMGRRAPR"
     gene            complement(2506..2802)
                     /locus_tag="P5201_05185"
     CDS             complement(2506..2802)
                     /locus_tag="P5201_05185"
                     /inference="COORDINATES: protein motif:HMM:TIGR03798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Nif11-like leader peptide family natural product
                     precursor"
                     /protein_id="HRV97960.1"
                     /translation="MSIESAKAFYERLETDTELQEKLKKLNTKENIEPYVKGELGYEF
                     TLEEMQKVIFEKNPEMTDEELEAVVGGSVIGTIGLILLAGPALFLGLAAGIAAA"
     gene            complement(2858..>3491)
                     /locus_tag="P5201_05190"
     CDS             complement(2858..>3491)
                     /locus_tag="P5201_05190"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRV97961.1"
                     /translation="RQWMIGDMVSFAHPEAEDLNGGALNRRLALLLAALWGVVPRSEE
                     LEAEEKRLRRRFKLKSDEAFAAWKKENDLDEEEMARLLEDEALLHKLHRWYMQGRIHG
                     KNTGAVLDELKLRGGYAEWKERAARRDALLHGRPEVLREERERACSTPFMTLVREHLR
                     ADGFPWHGAMLPEAIPETGLEPRDLVEELLLARAARRILGETTQFGTGEK"
BASE COUNT          652 a         1177 c          999 g          663 t
ORIGIN      
        1 gtcgccgggg tcgccttcca actctccacg ccgagcccgg cgaaaagctc caggcttgtc
       61 ggcagtgtgt gcagattccc ccggtagagc gccgtctcga tggagacggt aaaatttctc
      121 gaacgcagca gcctgatcgc gtcgatcgtc tgcctctccg tcccgggcac gccgcgcagc
      181 cagtcgtggc agccgatgcc gtcgaaactg agcaaaaact ccggacgaat tccccgggcc
      241 tcgaacttgt tcaggagcgc ctcgtccacc agcacgccgt tggtgtagac ctgtgagatc
      301 atgatgcgct catcgagcag ggcatccacg atatcgaaga aatcatttct gaccagcggc
      361 tcgcctccgg tcagcgacac gtccaggatg cccgcttcgg aaatctgacg cacaatgttc
      421 aggcactgct ccagcagcag ctccccgtac ttcgcgtgcg gcgcggacat gaagcagtgg
      481 cggcacttca ggttgcatct ccccgtgatc gaccagtggg ccttgcgcat gtacctgcac
      541 ggaatcagcc tgtacttttg ctcctccttc agcccttcgc cgaaggcgca ctcttcgacc
      601 atcccctgtt ctttcagcag cgagacggcc cggcgatgcg cggggagcag cagcggagag
      661 gccacgtcca ccatgccgtt gcagaatgat gcagcctgga acgccttctc gtccagaaag
      721 acgacgccgc cactcgcaac atcctgaatc gcgtacggca gctgttccca ccctctgagc
      781 ctataccgtt cctttagcgt gcaatacacc gcctcatcct ctcccctcgc gttccatcat
      841 cttcttcggt tcaggtactg gacgacgacc gaaagatcgc cccgggacga cgaaagacgg
      901 aaaggctttg catcgtcgac ttcagacgca gcatacacct ttacacagtg tcaaagtcaa
      961 gcggcatttt tcaaaagagt cgcaacctgc accgaattct atcatccgac ggagaaaaaa
     1021 gggggcggtt tattttctcc ccctcggaaa cgcagtatcg acaagtcttt gtagaattac
     1081 cgaaagaatg ctttccattc ggcccgaatg agcaacgtat ggttttcgac gggccgctgg
     1141 ggtagaatga tcgcagtgaa cgactattca ttcgatccat ttcaaggagg gtccatgaaa
     1201 aaacgcccgc tcctctatgt agtgtttttg cttccgctca ttcttccctg cgccgccttt
     1261 tccgcgcaca tggacgagat gcagtatctt gcgctcgtga tggagcggaa caacgacctc
     1321 gccgccgcca ggcaggagat cgaggcgcgc ctcttctccg cccgggcgga actggccatc
     1381 cagcggccgt cgctttcgct ctccgccgag acgagcaagt ggctcgacaa gaaccggggc
     1441 gaccagcagg ccgatctctc cctctcgcac cgcgtcaacc tctccggagc ctacggcctc
     1501 caggagcggg atctgctgct cggcgtcgag atccttcgga accagtacgc cgatgcggtc
     1561 aacgaacgcc tcgccctcgc cgcgtccgcc taccgccagg cggtgatggc cgcgctgaac
     1621 cgggaggcga tggaacagat cgtggaaaag cgccgcgagt cgctcacgat cacgaaggag
     1681 aagttcgaca aggagctggt ctccctgctg gagctgctgc gggcgcagtc gcaggtagac
     1741 gacggcgaag ccttcctcct ccaggcccgg cagaagtacg agcagcagct cgtggaaatg
     1801 agcgctctcg ccggaggggc tccggcgatt ccgtcggttg cgcttcccga actcggcgga
     1861 atgaatctcg tcgtggacga ggagaaggcg tgggacaacc gccccgacgt cgccgccctg
     1921 ctgctctcca aggaacgggc ttcggtgcag cgctcgctcg ccgcaaaggg gctctcaccg
     1981 ttcgtcgacc tctccgtcgg gctgagggtt ttggaggacc acatttttcc ccaggagcgg
     2041 ggcggagaag tcttcgttcg cgccgttctc acagttcctc ttgccgacgg cgggaagaca
     2101 tccaactcca ccgaagcggc gacgctgatg ctccgtaagg ccgagtacga gctggccgca
     2161 agacgcgaca gtctgaaacg ggaggccgac atggtgcggg agcgatggaa ccgcgcgctt
     2221 gaaatcgtgg ccatccgccg ccgtcaggcc gagcgcgcag ataaagagct cgaaatcgcc
     2281 gagctgatgt accgcgaggg aatgggctcc cagctcgacc tgatcaacgc ccaggaggcc
     2341 gaccagaaga gccggacgga gtttttaagc gccgtgcagg agctctggct cgtcctcgcg
     2401 gaggccgaca gggtcatggg ccgccgggcg ccgcggtaaa gctttcattc cctccgcact
     2461 taagaagtcc cgaaggcgcg tccgccttcg ggacttctta agtgatcagg ctgctgcaat
     2521 cccggcggca agccccagga acaacgccgg accggcaaga aggatcagcc cgatcgtgcc
     2581 gatcaccgag cccccgacga cggcctcaag ctcctcgtcg gtcatctccg gattcttctc
     2641 gaagatcacc ttctgcatct cctccagcgt gaactcgtac cccagttcgc ccttcacgta
     2701 cggctcgatg ttctccttcg tgttcagctt cttcagcttc tcctgaagct cggtatccgt
     2761 ctccaagcgt tcgtaaaacg ccttcgccga ttctatcgac atgcacgaca cctccctcga
     2821 attcgcctct ccctcgcgga aggggcggca gactccgtca cttctctccg gttccgaact
     2881 gcgttgtttc ccccagaatc ctccgggcgg cgcgggcaag cagcagctcc tccaccaggt
     2941 cgcgcggttc cagccccgtc tccggaatcg cctccggcag catcgccccg tgccacggga
     3001 agccgtccgc ccgcagatgt tcgcgcacca gcgtcatgaa gggcgtggaa cacgcgcgct
     3061 cacgctcttc acggagcact tctggccgtc cgtgcagcag cgcgtcgcgc ctcgcggcgc
     3121 gctccttcca ctccgcgtac ccgccccgga gcttgagctc gtcgagcacc gctccggtgt
     3181 tcttgccgtg aatacgcccc tgcatgtacc accggtggag cttgtgcagc agcgcctcat
     3241 cttcaagaag tcgggccatc tcctcttcgt cgaggtcgtt ctccttcttc catgcggcga
     3301 acgcttcgtc gctcttgagt ttgaagcggc ggcgaaggcg cttctcctcg gcctccagct
     3361 cctcgcttcg gggaaccacg ccccacagag cggcgagcag cagggcaaga cgccggttca
     3421 gcgccccgcc gttcaagtcc tccgcctcgg ggtgggcgaa gctgaccatg tcgccgatca
     3481 tccactggcg g
//
LOCUS       DAOTIL010000193         3474 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminobacteriaceae bacterium isolate AMR_MDS_3026
            NODE_32056_length_3474_cov_4.254460, whole genome shotgun sequence.
ACCESSION   DAOTIL010000193 DAOTIL010000000
VERSION     DAOTIL010000193.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868050
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminobacteriaceae bacterium (wastewater metagenome)
  ORGANISM  Aminobacteriaceae bacterium
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae.
REFERENCE   1  (bases 1 to 3474)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3474)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:19:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,224
            CDSs (total)                      :: 3,198
            Genes (coding)                    :: 3,184
            CDSs (with protein)               :: 3,184
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3474
                     /organism="Aminobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_32056_length_3474_cov_4.254460"
                     /isolate="AMR_MDS_3026"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:3034609"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(20..1018)
                     /locus_tag="P5201_05195"
     CDS             complement(20..1018)
                     /locus_tag="P5201_05195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005660209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH domain-containing protein"
                     /protein_id="HRV97962.1"
                     /translation="MREILFVSNTGNTTVVIFFVLFTVILVMSGIKIVPQASRVIVER
                     LGKFHKILEPGINFIFPVLDRRKATSWIVGGEIRNTTLIDIREQVFDFPKQNVISHDN
                     VVMEINALLYFQINDPFKAVYEIANLPLALEKLTQTSLRSVMGEMDLDEIFSKRAEIN
                     ERLRVILDEASDAWGVKVTRVEIQDVNPPASVQAAMQRQMEAERSRRAMVTEAEGKKT
                     AQILEAEGHKEAQINRAEGEKQAVILDAEGRARARIRMGEAEAEAVVKVASAVKIAGG
                     DPTQYLIALKYMDTLSSVASGNENKVVYLPYEATAILGSLGGMKELFSKDKTQTNA"
     gene            complement(1095..1571)
                     /locus_tag="P5201_05200"
     CDS             complement(1095..1571)
                     /locus_tag="P5201_05200"
                     /inference="COORDINATES: protein motif:HMM:NF014062.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NfeD family protein"
                     /protein_id="HRV97963.1"
                     /translation="MFELVTNWQLWVILGFALLIVEMFTPGFVLACFAIACIPPAIIT
                     YFGDFDLRAQLAVFGVSTLFIFFFMRPAVLRFLSNRKDARTSNAEALIGKKGIVIKTI
                     SPDAAQGGYVKVDGKTWWAFGPEGIEIGEGAGVVVVKVRGASLDVQFEENESSGEE"
     gene            complement(1629..2231)
                     /locus_tag="P5201_05205"
     CDS             complement(1629..2231)
                     /locus_tag="P5201_05205"
                     /inference="COORDINATES: protein motif:HMM:NF013933.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysE family transporter"
                     /protein_id="HRV97964.1"
                     /translation="MQWTAFFTYILITCNTPGPNVVLAMNTARIHGLRKTLPLIAGMM
                     SGLLLLMTFCAIFNLLLARLLPTLLPVLRTGGTVYILWLAWKIAFPKRNSLENAPAED
                     APDFRHGFVLQFLNPKVILLGLTTLSTFILPWTTSKTWLFGCGVFLTLACSNGVFLWS
                     LLGAAQKRLMTEQGRFWDYLMGGLLAWCAFSVSGLKELFF"
     gene            2469..3236
                     /locus_tag="P5201_05210"
     CDS             2469..3236
                     /locus_tag="P5201_05210"
                     /EC_number="1.1.1.47"
                     /inference="COORDINATES: protein motif:HMM:NF005559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose 1-dehydrogenase"
                     /protein_id="HRV97965.1"
                     /translation="MTFYDRFSVAGKVAVVTGAARGLGKGYALGLASAGATVLCGDVN
                     ENDAKLTAEEIRAFGGRAEGFFLDVTQPGKVEAVFSDAASRLGTLDIVVNNAGVEEIN
                     DFVSVTEEQYDKIMSVNLRGVFFTAQAAAKIMIKQRRGKIVNIGSLGSAIGLAQSSVY
                     CGAKGGVVQITRTMAIELAKYNIQVNAMGPGYFRTPMTEPFFQNPEHRRWIEERIPAG
                     RVGAAEDLLGTLVFLCSEASDYLTGQIVYIDGGWLAS"
BASE COUNT          840 a          907 c          916 g          811 t
ORIGIN      
        1 ccctcccttc tgtactgttt taagcgttcg tttgtgtttt gtccttagaa aaaagttctt
       61 tcatgccgcc gagcgagccg aggatcgctg tagcttcata aggcaggtag acaaccttgt
      121 tctcatttcc ggatgccact gaggaaaggg tgtccatata tttaagagcg atgaggtact
      181 gcgtgggatc gcctcccgca attttcacgg ccgaggctac tttcaccaca gcctcagcct
      241 cggcttctcc catgcgaatt ctggcccggg cgcgtccttc cgcatcaagg atcaccgcct
      301 gtttttctcc ctcagcgcga ttgatttgcg cctctttatg cccttccgcc tcaagtatct
      361 gcgcggtttt tttcccttcc gcctcggtga ccatggcccg cctggagcgt tccgcctcca
      421 tctggcgctg cattgcggcc tgaaccgagg caggcggatt cacgtcctga atctccactc
      481 tcgtcacctt taccccccaa gcatcgctgg cttcgtcgag gataacacgg agacgttcgt
      541 ttatttccgc gcgtttgctg aatatctcgt caaggtccat ctctcccatc acgctcctga
      601 gagatgtctg cgtcagtttt tccagagcca gcggcaggtt ggcaatttcg tacaccgctt
      661 tgaacggatc gttgatttgg aaatagagga gagcgttgat ctccatcacg acattgtcat
      721 gcgagataac gttttgcttt gggaagtcga aaacctgctc cctgatatca atgagcgttg
      781 tattccgtat ctcccctccg acaatccagg aagttgcctt tctccggtcg agaacgggga
      841 agatgaagtt aatgccgggc tcgagtattt tatgaaactt tccgagacgt tccactatga
      901 cacggcttgc ctgaggaacg atcttgatgc ccgacatcac caggatcacg gtaaacagaa
      961 cgaagaagat gacgaccgtt gtgttgcccg tgttgctgac gaacagaatc tctctcattg
     1021 ttttcacact ccccgtttat cggatgtgtt cctgataatt attcaagaat gatctagtca
     1081 gaacaccctg tgccttactc ttcacctgat gactcgttct cttcgaactg aacgtccagg
     1141 ctggctcccc gaacttttac gacgacgacc cccgccccct ctccgatttc gatcccttcg
     1201 ggaccaaacg cccaccaggt tttcccgtcc accttcacgt atcccccttg agcagcgtcc
     1261 ggcgagatgg tctttattac gatgcctttt ttcccgatga gcgcctcggc gttcgacgtg
     1321 cgggcatctt tcctgttcga cagaaatctg agaacggcag gccgcatgaa gaaaaagatg
     1381 aaaagagtag atacgccgaa gacagcgagt tgagcacgca gatcaaagtc tccaaagtat
     1441 gtgattatcg ccggagggat gcaggcgatg gcgaaacatg caagaacgaa gcccggagtg
     1501 aacatctcga cgatcagcaa ggcaaatccc agaatcaccc aaagctgcca gtttgtcaca
     1561 agctcgaaca tttttctcac ctcgactttc tcgaattgaa caacgtaccc tctccgatga
     1621 aaaaggcatc agaaaaacaa ctctttcagc cccgaaaccg agaatgcgca ccaggcaagc
     1681 aatccgccca taagataatc ccagaaacgt ccctgttctg tcatcagtct tttttgcgct
     1741 gcgcccagaa gagaccagag aaaaaccccg ttgctgcatg caagagtcag aaagacgccg
     1801 caaccaaaaa gccacgtttt cgaagttgtc cacggaagaa taaatgttga gagtgttgtc
     1861 agccccagaa gaataacttt agggttgaga aactggagta caaacccatg ccggaagtca
     1921 ggagcatctt cggctggagc gttttccagg gagtttcgtt tggggaaggc gatcttccag
     1981 gcaagccaga gaatgtacac cgtgccgccg gtcctgagca caggaagaag agtaggcaac
     2041 aggcgcgcaa gaagaaggtt gaagatggcg cagaaggtca tcagaagaag aaggcccgac
     2101 atcattccgg caatcaaggg gagcgttttg cgcaatccgt gaatcctcgc ggtgttcatt
     2161 gcgagaacta cattcggtcc cggagtgttg cacgttatga ggatgtaggt gaaaaacgcc
     2221 gtccactgca caagcgcacc tccaaagatg atgtttggcg aggcagaagc ccgctttgat
     2281 tttcatatta tacacgacga acgattccag cagagcagct tcacaaacaa gtagacttac
     2341 ggttttcacc gcttgcattg ttgaaagttt atgctaacat gatgaagcat catcaagaca
     2401 tctgttttct cctctctcag gaaagcgaaa gaacagacac cgcgaatcac aatcaaggag
     2461 cgaatcaaat gactttttat gatcgattca gcgtggccgg gaaagttgcc gttgttactg
     2521 gagcagcccg gggacttggg aaggggtatg cgcttggatt agcgtctgcc ggcgcgactg
     2581 tattatgcgg cgacgtgaac gaaaacgatg cgaagttgac ggctgaagag atacgagcct
     2641 tcgggggccg ggcagagggc ttttttctcg atgtgacgca gccgggaaaa gtggaagctg
     2701 tattttccga cgcagcctcc cgtcttggaa ctcttgatat cgttgtaaac aacgcgggcg
     2761 tcgaggagat caacgatttc gtctccgtta cggaggagca gtacgacaag attatgtcgg
     2821 tcaatctcag gggcgtcttc ttcacggcgc aggcagccgc taaaatcatg atcaagcagc
     2881 ggcgcggcaa aattgtcaat atcgggtcgc tggggagcgc catcggactg gcgcaatcct
     2941 cggtctattg cggcgccaag ggcggagtgg tgcagatcac ccggacgatg gctatcgagc
     3001 tggcgaagta caacatccag gtgaacgcta tgggtcccgg atacttccgt actcctatga
     3061 ccgagccgtt ctttcagaac cctgagcacc gccggtggat cgaggagcgg attccggcgg
     3121 ggcgagtcgg ggctgccgag gatcttctgg gaacgctggt ctttctgtgc agcgaagcct
     3181 ccgattattt gacgggacag atagtctaca tagacggcgg ctggctcgca agctgaggaa
     3241 attgtaatgt acaggagaga tgaacatctt ctcggctctc tctttttgct cccgctccaa
     3301 tagacgggag tttccagacc gatcaggagg aatgtgaagt gatcgtactc aaagaagcag
     3361 aacgtaattt tcaggaagaa ggaggctctg ttgaatctgg agtaggcgga attctcaaga
     3421 gtgtccgcga acgcggagac gaggctgttc tcgcctacac ggagaagttc gacg
//
LOCUS       DAOTIL010000194         3467 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminobacteriaceae bacterium isolate AMR_MDS_3026
            NODE_32172_length_3467_cov_3.306272, whole genome shotgun sequence.
ACCESSION   DAOTIL010000194 DAOTIL010000000
VERSION     DAOTIL010000194.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868050
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminobacteriaceae bacterium (wastewater metagenome)
  ORGANISM  Aminobacteriaceae bacterium
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae.
REFERENCE   1  (bases 1 to 3467)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3467)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:19:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,224
            CDSs (total)                      :: 3,198
            Genes (coding)                    :: 3,184
            CDSs (with protein)               :: 3,184
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3467
                     /organism="Aminobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_32172_length_3467_cov_3.306272"
                     /isolate="AMR_MDS_3026"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:3034609"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1068)
                     /locus_tag="P5201_05215"
     CDS             complement(<1..1068)
                     /locus_tag="P5201_05215"
                     /inference="COORDINATES: protein motif:HMM:NF014763.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cache and HAMP domain-containing protein"
                     /protein_id="HRV97966.1"
                     /translation="MKSIRTKIMASFIAATIVFMTIMGVTLYARVDGKVTDLTEDMAL
                     QISRASSVGLSEWLDNMVNLTGAFAGMDVVRSLEWKNAEAFLEGQMKKHHRLFEMLFI
                     SGTDGRVKGTGGTTADISQRPYFKELMSGGKQYAISDPVVSLATGNVIFVIAHAVPGA
                     DGKPAGVFAATVLLQNLQGLVDAVRVGETGFGWIVDGGGIVAAHRDKEHIGKFDVRQD
                     AAYTTVWKRMSAKETGYELHKRFDGTPAYVFFAPIAGSPGWTFAVTVLEREIKATAVD
                     LSWTIVIIVAFVIIAFAIISLLIGNSIARPVQTVQRLAERAKGGDLTILRSDFNIATK
                     DEIGRMADALADMIRVQREMVH"
     gene            1466..2104
                     /locus_tag="P5201_05220"
     CDS             1466..2104
                     /locus_tag="P5201_05220"
                     /inference="COORDINATES: protein motif:HMM:NF022655.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LmeA family phospholipid-binding protein"
                     /protein_id="HRV97967.1"
                     /translation="MLVSLTDPESMELRMDQQPDNDGNIRDFSIFIQGASIGGFRVEK
                     IAIESSFLELNRPSEWRTGERDSLKVRNVLRSNVELVALEKDINAALTSYAGGDWRRI
                     SVDLKKGMVSARGNYRSGSLDILAEVSTKLEIRQGKQIWLKDTAIKINHDDQTDVIQK
                     ELAKIQPVVDIGQFPFPVTLAVLKVDEQKLVFSTRTLPKPVKGIFYRYVRGS"
     gene            complement(2086..3270)
                     /gene="truD"
                     /locus_tag="P5201_05225"
     CDS             complement(2086..3270)
                     /gene="truD"
                     /locus_tag="P5201_05225"
                     /EC_number="5.4.99.27"
                     /inference="COORDINATES: protein motif:HMM:NF013322.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(13) synthase TruD"
                     /protein_id="HRV97968.1"
                     /translation="MRIKTAPDDFHVEEVLHFAPAKKEAPFRVFLLEKEGWNTADAIQ
                     EAAKACGVPLAEIRSVGKKDRHAHTIQHVSVPLKYDLAYEGKGFVIRFAGYSTAHLAP
                     SVLKGNRFSAALRDMTAAEAGTCEARLERIAARGFANYFDDQRFGNVGEKGEFFAEML
                     VKRRFGPALRKYFSFRHPEAPLPLKKRKAQLAALWGKWEEMVPLCPEPLLKQMLDILR
                     RKGESGYRACLKLIPAQEMGLYYSAYQSFLWNETLCRFLSKQPGELFPVPVKTGELLQ
                     YRMLDESLLSRLLSEPMHTAAPDMPQLPSDLGDVFSAVLFERGVRLSKFRLKEVEQAY
                     FSSFPRACAVIPENMTWKREEDALFPGKTALRVDFFLPRGSYATMMLKSAEEGGVQEP
                     RT"
     gene            complement(3295..>3467)
                     /locus_tag="P5201_05230"
     CDS             complement(3295..>3467)
                     /locus_tag="P5201_05230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005660926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HRV97969.1"
                     /translation="LYGVEIKLYSLRLELRKNLEVPVIERLFMAGDGAGVSRGIIQAA
                     ASGVVAARGALA"
BASE COUNT          718 a         1076 c          987 g          686 t
ORIGIN      
        1 gtgcaccatc tcgcgctgga cgcggatcat atccgcgagc gcgtcggcca ttcgcccgat
       61 ttcatccttc gtcgcaatgt tgaagtcgct cctgagaatc gtgagatcgc cgcccttcgc
      121 cctctcggca agacgctgga cagtctgcac gggccgggcg atcgagttgc ctatgaggag
      181 agagatgatc gcgaaggcga taataacgaa ggcgacgatg atcacaatcg tccacgaaag
      241 atcgaccgcc gtcgccttga tctcccgttc caggacggtg actgcgaacg tccagccggg
      301 cgatcccgcg ataggggcga agaaaacgta tgccggagtc ccgtcgaatc gtttgtgcag
      361 ctcgtaaccg gtctcttttg cggacatcct cttccatacc gtcgtatacg ccgcatcctg
      421 acgcacgtcg aacttcccga tatgctcctt gtcgcgatgc gcggcgacga tgccgccgcc
      481 gtcgacgatc catccgaacc cggtctcgcc cacccggacg gcgtcgacaa gaccctgcag
      541 gttctgcaga agcaccgtcg cggcgaagac tccggccggt ttgccgtcgg cgcccggaac
      601 ggcgtgcgca atgacgaaga tgacgtttcc ggtagccaga gagacgaccg ggtcggaaat
      661 cgcgtactgc ttcccgccgc tcatgagctc tttgaaatag ggtctctgtg agatgtccgc
      721 agtcgtcccc cccgttccct tgactcgtcc gtcggtaccg gagatgaaga gcatttcgaa
      781 aagacggtgg tgctttttca tctggccttc aagaaaggct tcggcattct tccactcgag
      841 cgaacgaacc acgtccatcc ccgcgaacgc tcccgtcaga ttcaccatgt tgtccagcca
      901 ctccgacaga ccgacggagc tcgccctcga aatctgcagg gccatatctt cggtaaggtc
      961 ggtcaccttg ccgtcgacgc gagcgtagag cgtcactccc ataatcgtca taaacacaat
     1021 ggtcgctgca ataaatgatg ccatgatctt cgtccgtatg ctcttcaacg cccatccacc
     1081 atcctttcag ccgtctctta caacgccgtc tccttcaccg caggattctc gaaggcgttt
     1141 ttgcacgcct gtatcgctca caacctcttc ccgcatttca aaatagatat agagcgcacg
     1201 ctgattttct gctagtatag cctaagaaac gcaagaggaa tagtctcgga aaagagagga
     1261 ggtttatttc ccggcgccat tccgaaggca ataaatagat agagcattct acattcaagg
     1321 aggaaaaatc atgagaaaaa caaccaatac cgttgcgctt gcgctcgcac tgctcttctt
     1381 tgcgatctcc gccttcgcgg agcagccaac cagggagttc tagggaagct tcgaacctgc
     1441 gaataacgga gaacgcctgc tctccttgct ggtttcgctg accgaccctg aatcaatgga
     1501 gcttcgcatg gaccagcagc cggacaacga cgggaatata cgcgactttt ccatcttcat
     1561 ccagggggct tccatcgggg ggttccgggt ggagaagatc gcgatcgagt cgtccttcct
     1621 ggagctcaac cgaccgtcgg agtggcggac gggggagcgg gattcgctca aggtcagaaa
     1681 cgtccttcgc agcaatgtgg aactcgtcgc gcttgaaaag gatatcaacg ccgccctgac
     1741 ctcctacgcc ggaggcgact ggaggcgcat ctccgtcgat ctgaagaagg ggatggtcag
     1801 cgcgcgcggg aactaccgct caggctccct cgatatcctc gcggaggtgt ccacgaagct
     1861 ggaaatccgg caggggaagc agatctggct gaaggatacc gcgatcaaga tcaaccacga
     1921 cgaccagacg gacgtcatcc agaaagagct ggcgaagatt cagcccgtgg tcgacatcgg
     1981 gcagttcccc ttcccggtga cgcttgccgt gctcaaagtg gacgaacaga agctcgtctt
     2041 ctcgacgaga acgcttccca agccggtcaa agggatcttc taccgctacg tccggggctc
     2101 ctgaacccct ccctcttcgg cgctcttgag catcatcgtc gcataacttc ctcgcgggag
     2161 gaagaagtcg acgcgaagcg cagtttttcc ggggaagagg gcatcctctt cccttttcca
     2221 tgtcatgttc tccggaatca cggcgcaggc gcgcgggaag gaggagaagt acgcctgctc
     2281 gacctctttc agccggaatt tcgagagacg gactccccgt tcgaaaagca ccgcggagaa
     2341 aacatcgccg aggtcggagg gaagctgcgg catatccggc gccgcggtgt gcatcggctc
     2401 ggaaagaagg cgcgaaagaa gcgattcgtc gagcatacgg tactgaagga gctcgccggt
     2461 tttcaccggc acaggaaaga gctcgcccgg ctgcttcgaa aggaagcggc agagcgtctc
     2521 gttccacaaa aagctctgat aggccgagta gtagagcccc atctcctgcg cgggtatgag
     2581 cttgagacag gctctgtacc cgctctcccc cttgcggcgg aggatgtcga gcatctgctt
     2641 caacagaggt tcgggacaga gcgggaccat ctcctcccac tttccccaga gagcggcgag
     2701 ctgtgccttt ctctttttga gcggcagggg cgcttcaggg tgccggaagg agaagtactt
     2761 cctgagggcg ggtccgaagc gccgttttac cagcatctcg gcgaaaaact ctcccttctc
     2821 tcccacgttg ccgaagcgtt gatcgtcgaa gtagttggcg aagccgcgcg ctgcgatccg
     2881 ctccagccgc gcctcgcacg tcccggcctc ggcggctgtc atgtcgcgga gcgcagcgga
     2941 gaagcggttg cctttcagca cggacggcgc caggtgcgcc gtggagtatc ccgcaaagcg
     3001 gatgacgaac cccttcccct catacgcaag atcgtacttc aacggcacgg aaacgtgctg
     3061 aatcgtgtgc gcgtgccggt ctttcttccc taccgagcgg atctcggcga gcgggacgcc
     3121 gcaggccttc gccgcttcct gaattgcatc ggcggtgttc cacccctcct tctcaagcag
     3181 gaagacgcgg aacggcgcct ccttcttcgc cggagcaaag tgaagcacct cttccacatg
     3241 aaaatcgtcg ggagctgtct tgattcgcat ctgtcttctc ctctgctgcg ggcgctaagc
     3301 cagcgcgccg cgcgcggcga cgacgccgct ggccgcggcc tgtatgattc cccggctgac
     3361 ccccgcgccg tcgccggcca tgaagaggcg ttcgatgacc ggcacctcca ggttcttccg
     3421 aagctccagc cgcagcgagt acagcttgat ctccaccccg tagagca
//
LOCUS       DAOTIL010000195         3455 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminobacteriaceae bacterium isolate AMR_MDS_3026
            NODE_32303_length_3455_cov_4.135588, whole genome shotgun sequence.
ACCESSION   DAOTIL010000195 DAOTIL010000000
VERSION     DAOTIL010000195.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868050
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminobacteriaceae bacterium (wastewater metagenome)
  ORGANISM  Aminobacteriaceae bacterium
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae.
REFERENCE   1  (bases 1 to 3455)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3455)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:19:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,224
            CDSs (total)                      :: 3,198
            Genes (coding)                    :: 3,184
            CDSs (with protein)               :: 3,184
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3455
                     /organism="Aminobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_32303_length_3455_cov_4.135588"
                     /isolate="AMR_MDS_3026"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:3034609"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1754)
                     /locus_tag="P5201_05235"
     CDS             complement(<1..1754)
                     /locus_tag="P5201_05235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013048206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit A"
                     /protein_id="HRV97970.1"
                     /translation="MAAEKVIKGTIEKISGPLVVAKGMFGAGMYDVVRIGEIGLVGEI
                     IELKGDTASIQAYEETSGLMPGERVVSTGEPLSVELGPGLIEQFYDGVQRPLNLIEDA
                     AKSHYISRGIDVPAIDRKKKWEFTPKVKAGDAVEQGDILGVVQESVLVEHRIMVPVGL
                     GGKVVKIEKGSFTVEEVVAVLEQNGEKKNVTMLQRWPVRKARPVAKRLPPVIPMTTGQ
                     RVIDTFFPIAMGGTACVPGPFGSGKTVVQHQLAKWAEAEIVVYVGCGERGNEMTDVLL
                     EFPELEDPRSGHPLMKRTVLIANTSNMPVAAREASIYTGITMAEYYRDMGYSVALMAD
                     STSRWAEALREMSGRLEEMPGEEGYPAYLGTRLASFYERAGRAIVLGGDKREASVSAI
                     GAVSPPGGDLSEPVSQNTLRVAKVFWGLDATLAYQRHFPAINWLLSYSLYTEKLDEFW
                     DVKFDDEWSPTRIEAMTLLEDEDKLREIVRLVGVDALSKEERMVLETAKSLREDFLHQ
                     NAFHEIDTYASMEKQFKMLKTILAFHHISMDALKKGASMNEVFNLPVREAIARMRYLE
                     EKDIGKIDDIEGQIKNEVA"
     gene            complement(1782..2123)
                     /locus_tag="P5201_05240"
     CDS             complement(1782..2123)
                     /locus_tag="P5201_05240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008522367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0034220 - monoatomic ion transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit F"
                     /protein_id="HRV97971.1"
                     /translation="MAVKKTGSPMAAVGEYETVLPFQAVGVKPCIVKDDETSDVGAIL
                     LELAKAGFAVVFVQESFFVGHTRRIEELNDEYETSFIPIPGLKGSEGVGLESIRSSVE
                     RAVGMDIFAVK"
     gene            complement(2113..3126)
                     /locus_tag="P5201_05245"
     CDS             complement(2113..3126)
                     /locus_tag="P5201_05245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009165700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATPase subunit"
                     /protein_id="HRV97972.1"
                     /translation="MAPAERYAYAVARLRAMSGRLLEEALIQRILDSEDLDSALKVLG
                     ETTYSSWLVELKGGADFDRVIESELLHVYTEVQKFVPDPRLVHLCRIPYDFHNVKVLL
                     KSLILAREGGERRFDLLTSLGVLPTDDLIMAMESEDYRLLPFGLHRLVPLCLSHWEQT
                     KDILQVENILDDGLFSVLSEMAAATGFDSATRWVKGRIDAENIRNLLRLKRMEMDTGN
                     IASFLHGGGWISREKLLALLPEPVESWPRILSFADVASAFSHIQDLSDLGVLLVEMEK
                     VLDEYVSSILARAKYDAFSPENVIAFLWMKELEAKNLRIAMVSVANNTDRNLARGLLR
                     HGR"
     gene            complement(3143..>3455)
                     /locus_tag="P5201_05250"
     CDS             complement(3143..>3455)
                     /locus_tag="P5201_05250"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRV97973.1"
                     /translation="KTLASMPGEAYISFIGKLFKKAVETGNEKVYISKSEKNISSEWI
                     SKLNAQNGWSLSLSEERRDISGGFILEQDNIETNCSFDMLVRWIRDEIEADVVKRLFS
                     A"
BASE COUNT          813 a          969 c          799 g          874 t
ORIGIN      
        1 gcgacctcgt tcttaatttg gccttcgatg tcgtcgatct tcccgatgtc cttctcttca
       61 agataacgca tgcgggcgat agcctccctg acaggaagat tgaaaacttc attcatagag
      121 gcgccctttt tgagggcatc catcgagata tgatggaatg caagaattgt cttcagcatc
      181 ttgaactgct tttccatgga agcgtacgtg tcaatctcgt ggaatgcgtt ttgatgaagg
      241 aagtcctctc ggagcgattt tgccgtttcc agtaccatcc gttcctcttt agagagtgcg
      301 tcgacgccga caaggcgcac aatttcacgc agtttgtctt catcctcaag aagcgtcatg
      361 gcttcgatac gggtgggact ccattcatcg tcgaatttga catcccagaa ttcatcgagt
      421 ttttccgtgt acaacgaata gctcaaaagc cagttgatcg ccgggaagtg gcgttggtaa
      481 gcaagcgtcg catccaatcc ccagaatacc tttgctacgc gaagcgtatt ctgacttacc
      541 ggttcggaaa ggtctcctcc cggaggtgaa actgcgccta ttgcactgac ggatgcctct
      601 ctcttgtctc cgcccaggac gatcgccctg cccgctcttt cgtagaagga ggccagtctg
      661 gtaccgaggt atgccggata cccctcttcg ccgggcattt cttcaagacg tcccgacatc
      721 tcgcggaggg cctcagccca acggctcgtt gaatctgcca tgagggcgac ggaatacccc
      781 atatcacggt agtactcggc cattgttatt cctgtgtaga tacttgcttc gcgtgctgcg
      841 acaggcatgt tggaagtatt ggcgatcaac acagtccgtt tcatgagcgg atgtcctgaa
      901 cgcgggtctt ccagttcggg gaattcaagg agcacgtctg tcatttcgtt tccgcgttct
      961 ccgcagccga catagacgac aatctccgcc tccgcccatt tcgcgagctg gtgctggaca
     1021 acggtctttc cagaaccgaa cggaccgggt acgcacgcag ttccgcccat agcgatcggg
     1081 aagaatgtat cgatgactct ttgtcctgtt gtcatcggaa taacaggagg aagacgcttc
     1141 gcaacgggcc gtgcttttcg aaccggccac ctttgaagca ttgttacgtt ctttttctcg
     1201 ccgttctgtt caagaaccgc gacaacttcc tctacagtga aagagccttt ttcaatcttg
     1261 actactttgc cacccaagcc taccggcacc atgatgcggt gctccacgag aaccgactcc
     1321 tgcacaacgc caagaatatc gccctgttcc actgcgtcgc ccgcttttac tttcggggtg
     1381 aactcccatt ttttctttct gtcgatggcg ggtacgtcga tacctcggga gatataatga
     1441 ctctttgccg cgtcttctat cagattcaaa ggtctctgta cgccgtcgta gaactgttct
     1501 ataaggccgg gccccagttc gacgctcaaa ggttcccctg tactgacaac cctctctccg
     1561 ggcataaggc cggaggtctc ttcatacgcc tgtatagatg ccgtatcgcc cttaagctcg
     1621 atgatttctc cgacgagtcc gatctcgccg atccgcacca cgtcgtacat gccggcgccg
     1681 aacatacctt tcgccactac aagggggccg gatatttttt cgatagttcc ttttatgact
     1741 ttctcagcgg ccacaatcta tcgcctccaa acttctattc attacttcac tgcaaaaata
     1801 tccattccga ctgctcgctc gacgctgctt cgaatggatt ccaaaccgac tccctcgctc
     1861 ccttttagcc cggggatggg gatgaaactt gtttcgtact cgtcgttcag ttcttcgatc
     1921 ctacgggtgt gcccgacgaa gaaagactct tggacgaaga cgaccgcaaa gcctgctttt
     1981 gcaagctcaa gaagaattgc gccgacgtcc gaggtttcat catccttgac aatgcacggc
     2041 ttcactccga ccgcttgaaa aggaaggact gtttcgtact cgccgacagc ggccattgga
     2101 gaacctgttt tcttaacggc catgacgaag taaccccctc gcaagatttc tgtccgtatt
     2161 attcgctaca gagaccatcg caatacggag attcttagcc tcaagctcct tcatccaaag
     2221 gaaggcgatc acattttccg gcgagaacgc atcgtatttt gctcttgcga gaatgcttga
     2281 cacatactcg tcaagaactt tttccatctc caccaagagg acgccaaggt cggaaaggtc
     2341 ctggatatgt gagaaagcgc ttgccacgtc cgcgaaagaa agaattctcg gccagctttc
     2401 cacgggttca ggaagcagag cgaggagttt ctctcgcgat atccagccgc ctccatgcag
     2461 aaatgatgca atattccccg tgtccatttc catacgcttt aaccgaagaa ggttacgaat
     2521 attctcagcg tcgatcctgc cctttaccca acgagtagcg gagtcaaaac ctgtcgctgc
     2581 cgccatttcg gataagacag agaaaagacc gtcgtcaaga atgttctcga cttgaagaat
     2641 atctttcgtc tgttcccaat gcgaaaggca taacggaaca agacgatgaa ggccaaaggg
     2701 gagaagacgg tagtcttcgc tctccattgc cataatcaaa tcatccgtgg ggagaacgcc
     2761 aagagaagtt aaaagatcaa accggcgctc tccgccttcc cgtgcaagaa tcaaactttt
     2821 taaaagtact ttcacattat gaaagtcata aggaatacgg cacagatgca caagacgagg
     2881 atcgggcacg aatttctgca cttcggtata cacatgcaaa agctcggatt cgattactct
     2941 gtcgaagtca gctccgcctt tcaattcaac gagccaagat gagtatgtcg tctcgcccag
     3001 aactttcagt gctgaatcga ggtcttcact gtccagtatc cgttggatca acgcttcttc
     3061 tagaagacgt ccggacattg cccggagtcg cgcgaccgca taggcataac gctcagccgg
     3121 agccaaggag cgtcacctcc gatcacgccg aaaacagccg cttgacaacg tctgcctcga
     3181 tttcgtcccg aatccaccgg acaagcatat caaacgaaca gtttgtctcg atattgtcct
     3241 gttccagtat gaatccaccc gatatatccc ggcgttcctc actcagagaa agcgaccatc
     3301 cattctgggc attgagcttc gaaatccact cggaagagat gtttttttcg gacttcgaaa
     3361 tatacacttt ttcatttccc gtctccactg cttttttgaa cagcttgccg ataaaagaaa
     3421 tatatgcttc tccaggcatg gacgcgagcg tcttc
//
LOCUS       DAOTIL010000196         3445 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminobacteriaceae bacterium isolate AMR_MDS_3026
            NODE_32435_length_3445_cov_4.269912, whole genome shotgun sequence.
ACCESSION   DAOTIL010000196 DAOTIL010000000
VERSION     DAOTIL010000196.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868050
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminobacteriaceae bacterium (wastewater metagenome)
  ORGANISM  Aminobacteriaceae bacterium
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae.
REFERENCE   1  (bases 1 to 3445)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3445)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:19:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,224
            CDSs (total)                      :: 3,198
            Genes (coding)                    :: 3,184
            CDSs (with protein)               :: 3,184
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3445
                     /organism="Aminobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_32435_length_3445_cov_4.269912"
                     /isolate="AMR_MDS_3026"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:3034609"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            48..935
                     /locus_tag="P5201_05255"
     CDS             48..935
                     /locus_tag="P5201_05255"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HRV97974.1"
                     /translation="MREVRVPEGPFPQHLREKEAREDTYASASILVVEDNPTNRTVAV
                     ALLNLLGLQNIETAENGEEAIEKLSATQYDLVFMDIQMPLLDGLQALAKIRDPSSKVV
                     DHETPVVAMTANALPGERERYIEAGMSDYISKPILAAELRRIVRRFLSEDKRDSRHSD
                     SGRFVLSEANMKSPDSSEIPAFRPAVLLDRMGGDRDLCRQTITLFIEDSAKIVKIISS
                     SVASGNAEESRRMIHALRGASANVEAFALALLAADAEQAAERGDLEEAGRALPKIERA
                     REKFIHAFREFDTPISDTK"
     gene            981..1910
                     /locus_tag="P5201_05260"
     CDS             981..1910
                     /locus_tag="P5201_05260"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_process: GO:0009966 - regulation of signal transduction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HRV97975.1"
                     /translation="MKILVAEDDATSLFMLQSLLMKWGYDVIAASDGQEALRVLTGEN
                     PPLLAILDWMLPGESGPEICRKLASRERLREGNAAREEMMKPYQYVIILTVKGEKENV
                     VAGLEAGADDYITKPFDAHELKMRLMVGRRILDLQEKLRNAALFDALTGLLNRRAVIE
                     RLEQELARASREATPLSVALLDIDHFKDVNDTYGHASGDAVLVESAQRIRSSVRPYDI
                     VGRYGGEEFLMVFPGAEGSALDGICERIRNAFQGAPFYSAENDGDRSTMDITVSIGIS
                     EYKKDNDNVDFILAEADRAMYRAKRSGRNRVSR"
     gene            2285..>3445
                     /locus_tag="P5201_05265"
     CDS             2285..>3445
                     /locus_tag="P5201_05265"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRV97976.1"
                     /translation="MIALALERAGQKNEARKMALALLPKAPADLGYYVAYALFRLADE
                     KDHDGRKKYLQRMYALAENNAQRTTALTNLLKLPGNKEAYALNLTNISPRNSAALKVL
                     ESLPKPWNPDVNMAVGYAAYLRGQYDKAIPLFRAIPMDSRHGRKSRYYRAFSLYNQKK
                     YTAALEAWGYLAKTGTSYAESSIRRISILAGRAERESALSVLREVAASRDGEIQARAY
                     YSLSTHLSGKEKREAEDKVIALAPNSLFTTQILWRRGWGKWASGDVRGAVADWERSLV
                     PEMKTSWRPRVLYWVSKGYGRLNQPSKRDTFVKTLLKDHPLSIYAFLSGGEKLDIQAG
                     DPPSLASRQPSELEKWGFVSHARRVLLAKGDAPSIYRAALLAEWMGDQQAAYS"
BASE COUNT          835 a          834 c          987 g          789 t
ORIGIN      
        1 tggagagccg cgagggggag ggctccctct tttccttcac tattcccatg cgcgaggtcc
       61 gagttcccga agggcccttt ccgcagcact tgcgggaaaa ggaggcgcga gaagatacgt
      121 atgcgtcggc ttccatactc gttgtggagg ataacccgac aaacaggacc gtggcggtcg
      181 cactgctcaa tttgctcggt ctgcagaata tcgaaacggc tgaaaacggt gaagaagcta
      241 ttgaaaagct ctctgcaaca caatatgatt tagtcttcat ggatattcag atgccgttgc
      301 tcgatggact tcaggcgctt gccaaaattc gggatccatc gtccaaggta gtggatcatg
      361 agactcctgt ggtagcgatg acggcaaatg ctctccctgg ggaaagagag cggtatattg
      421 aggcggggat gtcagactac atatccaaac cgattcttgc cgcggaacta cgaagaatcg
      481 tccggcgctt tctttcagaa gataaacgtg actcccggca ttctgattct gggaggttcg
      541 tactctctga agccaacatg aaaagccccg attcgtccga gattcccgct tttcgccctg
      601 cggttctgct ggatcggatg gggggagatc gcgatctgtg ccggcagacg attactcttt
      661 tcatagagga ctcggcgaaa atcgtgaaaa taatcagctc ttcggtagcg tcggggaatg
      721 cggaggaatc acgccggatg atacacgcgc ttcggggcgc ctcggcaaat gtggaagctt
      781 ttgctcttgc gcttttggct gccgatgcgg aacaggcggc ggagcgcgga gatcttgaag
      841 aagccggacg ggctcttcca aagattgaga gggcgcgcga gaaattcatt cacgcctttc
      901 gcgaattcga tacacccatt tctgatacaa agtaaatgcg ctccgatata cgtgtgttta
      961 aggaaaaagg agagattcgg atgaaaatat tggtcgcaga ggacgacgcg acatcgctct
     1021 tcatgcttca atcgctgctc atgaaatggg ggtatgacgt tattgcggcc tcggacgggc
     1081 aggaagcgct gcgcgtgctg acaggagaaa acccgccgct gctggcgata ctcgactgga
     1141 tgcttcccgg tgaaagcggc cccgaaattt gccgaaaact cgcctccaga gagcgcttaa
     1201 gggaggggaa cgccgcccgg gaagagatga tgaaaccata tcaatacgtt atcatcctca
     1261 ccgtgaaagg agagaaggaa aatgtggttg ccggtttgga agcaggagcc gacgactaca
     1321 taacaaaacc ctttgacgca catgaactga aaatgcggct catggtaggg aggcgcattc
     1381 ttgaccttca agaaaagctt cgcaacgccg ctcttttcga tgcattaaca ggacttctca
     1441 acagaagagc cgtcattgag cgtcttgaac aagaactggc gcgggcatcc agggaagcaa
     1501 ctcctttgag tgttgcgctt ttagacattg atcattttaa ggatgtcaac gatacttacg
     1561 ggcatgcctc gggagatgcg gtgctggtgg aaagtgcgca gcgaatacgt tcttcagtcc
     1621 gtccgtacga tattgttggc agatacggcg gggaagaatt tctcatggtt tttcccggcg
     1681 ccgagggaag cgcgctggat ggtatctgcg aaagaatacg gaatgctttc cagggagctc
     1741 cattttattc ggccgagaat gacggtgatc ggtcaacgat ggatataaca gtgagtattg
     1801 gtatttctga atataaaaag gataatgata atgttgactt tattctggct gaagcagaca
     1861 gagcaatgta cagagcgaag agaagcggga gaaatagggt aagccgctga ctactgtcgg
     1921 taattctacc ggagcggcag gaagctctct cacgtagaat ggccgggata ttcgccgaaa
     1981 ggtgttgaga ggagtgtttt cggtgtcccg acgattgttt cgtctttctt tgttttttgt
     2041 gttctctgcg ttgctttttt tcttcccttt gagtgcatac agccagtcaa tgggagatct
     2101 tttccaacgc cgggactgga agggaattga ttcccttctt gtttcgggaa atgctctcac
     2161 ggccgtggat tactcacttg ccgccaatgc tctgtggttc cagaatcggt ggagtgaatc
     2221 acttgttact ctcaaaaaga gcgcatcttc atggccatcc ggggtaaaac catacgggga
     2281 ttttatgatt gcacttgccc tggagagggc aggacaaaaa aatgaagccc gtaaaatggc
     2341 tcttgcactc ttacctaaag ctcctgcaga tctcgggtac tatgtggctt acgctctttt
     2401 tcgcctggcc gatgaaaagg atcacgacgg aaggaagaaa tacctccagc ggatgtacgc
     2461 tcttgcggaa aacaacgcac agcgcacgac tgctctgaca aatcttctaa agcttccggg
     2521 caacaaagag gcgtacgctc tgaacctgac gaacatctcg cctagaaaca gcgccgcttt
     2581 aaaagttttg gagtctcttc ccaaaccatg gaatcccgac gtcaatatgg ccgtgggata
     2641 cgcggcgtat ctccgggggc agtacgataa ggcgattccc ctcttccggg caattccaat
     2701 ggattcaaga cacggcagaa agtcgcgcta ctaccgtgcg ttttcattgt ataaccagaa
     2761 aaagtacacc gccgccctgg aggcgtgggg atacctcgcg aaaacaggga cgagttatgc
     2821 cgaatcttcg atccggcgga tttctattct tgcagggcgg gcggagagag aaagcgctct
     2881 cagcgtgctc cgggaagttg ctgcgtccag agatggcgag atccaggccc gggcctatta
     2941 ttccctctct acccaccttt ccgggaagga gaaacgggag gcggaagaca aagttatcgc
     3001 gctcgctccc aactcgctgt tcactacgca gatactgtgg agaagaggat ggggcaagtg
     3061 ggcttcagga gatgtccgcg gggcggttgc cgattgggag agaagccttg tccccgagat
     3121 gaaaacgagc tggcggccga gggtgctcta ctgggtctcc aaggggtacg ggcgtttgaa
     3181 tcagccctcg aagagagata cgttcgtgaa gacgctcctc aaagatcacc ctctctccat
     3241 atacgccttt ctgagcggcg gggagaagct cgatatacag gccggagatc ctccctcgct
     3301 tgcatcccgc cagccttcag agcttgaaaa gtgggggttt gtctcccatg cgagacgcgt
     3361 tctcctggcg aaaggagatg ctccttcgat ataccgtgcg gcgctccttg cggagtggat
     3421 gggagaccag caagccgctt actcc
//
LOCUS       DAOTIL010000197         3440 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminobacteriaceae bacterium isolate AMR_MDS_3026
            NODE_32502_length_3440_cov_3.487740, whole genome shotgun sequence.
ACCESSION   DAOTIL010000197 DAOTIL010000000
VERSION     DAOTIL010000197.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868050
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminobacteriaceae bacterium (wastewater metagenome)
  ORGANISM  Aminobacteriaceae bacterium
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae.
REFERENCE   1  (bases 1 to 3440)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3440)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:19:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,224
            CDSs (total)                      :: 3,198
            Genes (coding)                    :: 3,184
            CDSs (with protein)               :: 3,184
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3440
                     /organism="Aminobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_32502_length_3440_cov_3.487740"
                     /isolate="AMR_MDS_3026"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:3034609"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1546
                     /locus_tag="P5201_05270"
     CDS             <1..1546
                     /locus_tag="P5201_05270"
                     /inference="COORDINATES: protein motif:HMM:TIGR03768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="TIGR03768 family metallophosphoesterase"
                     /protein_id="HRV97977.1"
                     /translation="GYGDWTYGPGLPHDRRLDLMPKEYGGNDATQKTRLLNFFTISDI
                     HITDKESPNQLIHLQNLHPESRYGSSLYSGVMLYTTHVLDAAIQTVNALHKQNPLDFG
                     ISLGDACNTSQYNELRWYIDVLDGKVIRPSSGAHLGAETIDYQKPYKAAGLDTSIPWY
                     QTMGNHDHFWMGSFPLEHRLRKDLRQSYVSDDVFATGDLLSDPRKIGSRDYYMGVLDG
                     STPYGDVKYAGPVGDFKTQPKVAADPDRRSILRTEWMQEFFNTSSLPAGHGFDMPGAK
                     DGFACYSFVPKPNVPLKMIVLDNTQRDDSGSLDIHGHGFLDAARWEWLKKELADGTAA
                     GQLMVIASHIPISVEVTAPNSSMGWWTDPKNAVTLPDLLAELHSHPNLVLLMAGHRHL
                     NTVKAFVSPDPAGAPENGFWQVETSSLHDFPQQFRTFEIFLNSDDTISIVTTNVDPAV
                     LEGTPAAASRRYAIAAAQILKNMEGIEHWNPTNDPTVKPMPAGSYNAELVVPLSPAMR
                     EKLKNF"
     gene            complement(1617..1778)
                     /locus_tag="P5201_05275"
     CDS             complement(1617..1778)
                     /locus_tag="P5201_05275"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRV97978.1"
                     /translation="MENSTRGTRTSGSGNIGDCARTQDLFRRRGNLERAARRFFPKRL
                     KHNYGFDLR"
     gene            complement(1931..2674)
                     /locus_tag="P5201_05280"
     CDS             complement(1931..2674)
                     /locus_tag="P5201_05280"
                     /inference="COORDINATES: protein motif:HMM:NF016442.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PaeR7I family type II restriction endonuclease"
                     /protein_id="HRV97979.1"
                     /translation="MTLNLVDFERKARSAVESFWKNREAAKLKQIESGNADHGERAGV
                     TAGKNMDGFLALMLDVIRNNGLKDAEIHQGRTMLTLPGYFRPTKLWDLLVVHNGVLVA
                     AIELKSHVGPSFGNNFNNRTEEAIGSACDFWTAFREGAFGAQPRPFVGWLMLVEDAPG
                     SSSPVKDNSPHFPVFEEFQGASYLKRYELLCRRLMQEGLYTTAAVLSTPRNALETEAY
                     SELSPMTGLRQFVAALAGHVAAEAARIGG"
     gene            complement(2671..>3440)
                     /locus_tag="P5201_05285"
     CDS             complement(2671..>3440)
                     /locus_tag="P5201_05285"
                     /inference="COORDINATES: protein motif:HMM:NF024352.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="TaqI-like C-terminal specificity
                     domain-containing protein"
                     /protein_id="HRV97980.1"
                     /translation="SDRTDLIRRLEENFPSLEETGCSVGIGVATGADKAFIGKYDALD
                     VEPDRKLPLVTTKDIVSGEVRWLGRGIVNPFADGGGLVDLKAYPRLGRYLEERRDVIA
                     RRHCAQKSPDNWYRTIDRITPSLTWRPKLLIPDIKGEAHIVYEQGELYPHHNLYYVTS
                     NEWELRALQAVLLSAISRLFVTTYSTKMRGGYLRFQAQYLRRIRIPRWSDVSGVLRKE
                     LTEAAIARDIHACNNATFRLYGLNDEEQATLGGNGTR"
BASE COUNT          731 a         1007 c          947 g          755 t
ORIGIN      
        1 cggctacggc gattggacct acggcccggg gcttccccac gacagacggc tcgatctcat
       61 gccgaaggag tacggcggca acgacgccac tcaaaaaaca cggctgctga atttttttac
      121 catatcggat atccatatca ccgacaaaga atctcccaat cagctgatcc atctccaaaa
      181 cctgcatcct gaatcacggt acggatcttc tctgtattcc ggggtcatgc tgtacacaac
      241 gcacgtgctc gacgccgcca tccagaccgt caacgcgctg cacaagcaga accctctgga
      301 ctttggtatt tctcttggag acgcctgcaa cacctcgcag tacaatgaac ttcggtggta
      361 tatcgacgtt ctcgacggca aggttatccg gcccagctcc ggcgcgcatc tgggagccga
      421 aaccatcgat tatcagaaac cctacaaagc ggcggggctc gacacgtcca ttccctggta
      481 tcagacaatg gggaaccacg atcatttctg gatgggctcc ttcccgctgg agcacagact
      541 gcgcaaggat cttcggcaat cctacgtcag cgatgacgtc ttcgccacgg gagatctcct
      601 gagcgacccc cggaagatcg gcagtcgcga ctactatatg ggggtgctcg acggctcgac
      661 gccctacgga gatgtcaaat acgcgggccc tgtcggcgat ttcaagaccc agcccaaagt
      721 cgccgccgat ccggatcgcc gttcgatttt gcgaacggag tggatgcagg agtttttcaa
      781 cacgtcgtcg cttcccgccg gtcacgggtt cgacatgccc ggcgccaagg acggctttgc
      841 atgctacagc ttcgttccga aaccgaatgt tccgctcaag atgatcgtgc tggacaatac
      901 gcaaagggat gacagcggct ccctcgatat tcacgggcac ggctttctgg atgcggcgcg
      961 ctgggaatgg ttgaaaaagg agcttgccga tggcacagcg gccggtcagc tgatggtcat
     1021 cgcctcgcac atcccgatca gcgttgaagt gacagcgccc aattcatcga tgggctggtg
     1081 gaccgatccg aaaaatgccg tcacactgcc tgatctgctg gcggaactgc acagccatcc
     1141 gaacctcgtc ttgttgatgg ccgggcatcg tcatctgaat accgtgaaag ccttcgtgtc
     1201 gccggatcct gcgggcgcgc ccgaaaacgg cttctggcag gtggaaacgt cgtcgcttca
     1261 cgattttccg cagcagtttc gcactttcga gattttcctc aacagcgacg ataccatttc
     1321 catcgtgaca acgaacgtcg atcccgcagt tcttgaaggg acgcccgccg cagcctcgcg
     1381 cagatatgcc attgcggcag cgcagattct caaaaacatg gaaggaatcg agcattggaa
     1441 tccaacaaac gatcccacag tgaagcccat gccggccggg tcgtacaacg cggagctggt
     1501 ggttcccctg agcccggcga tgcgcgagaa gctgaaaaac ttttgacgga gacgaatgca
     1561 ccccgaaaac cggggggagg gtacgccctt aaataatcag agcgcgtcgg aacttttcag
     1621 cggaggtcaa agccgtagtt atgcttgagg cgcttcggga agaagcggcg agccgcgcgt
     1681 tccaagttcc ctcggcggcg aaaaagatcc tgtgtgcgcg cacaatctcc gatattgccg
     1741 cttccggatg ttcgggtgcc gcgggtactg ttctccatga cgacaaccct cctttgcaag
     1801 ggttgtcgtc agttgttaca acctcttttt gaaagagagt atgcctttcc cgttggctag
     1861 aggagtttag aaatggacgc ataccagttt ccgactgagc gcttggacga ggacgcttca
     1921 catccagtca tcaaccccct attcgtgcgg cttccgctgc gacgtgtccg gccagtgccg
     1981 cgacaaattg tctcagcccc gtcatgggag aaagttcgga gtaggcctct gtttccaagg
     2041 cgtttcgcgg tgtcgagagc actgcggcgg ttgtatagag tccttcctgc atcagacgcc
     2101 ggcacagcaa ctcgtaccgt ttcaggtatg aagctccctg gaattcttcg aacacaggaa
     2161 aatggggaga gttgtccttt acgggtgaac ttgatccggg agcgtcttcg accagcatca
     2221 gccagccgac gaacggccga ggctgtgcgc cgaaagctcc ttcccgaaat gccgtccaga
     2281 agtcgcacgc actgccgatg gcttcttcgg tccggttgtt gaaattgttg ccgaaggaag
     2341 ggccgacgtg gcttttcagt tcgatcgccg caacgagcac tccgttatgg actacaagca
     2401 agtcccagag ttttgtaggt cggaagtagc ccggcagagt cagcattgtc cgtccttggt
     2461 gaatttcggc atccttcagc ccgttgttcc tgatgacatc cagcatcaac gccagaaaac
     2521 cgtccatatt cttgccggct gtgacgcctg ctcgctcgcc gtgatcggcg ttgccggatt
     2581 cgatctgctt gagtttcgcc gcctctcggt ttttccaaaa actttctact gcgctacggg
     2641 ctttccgttc gaaatcgaca agattcagcg tcatcgagtc ccgttccctc ccaaagtagc
     2701 ctgttcttcg tcgttcaggc cgtacagcct aaaggtcgcg ttgttgcagg cgtggatatc
     2761 tcttgcgatc gccgcttccg taagttcttt tcttaaaact ccggagacgt ccgaccatcg
     2821 cggaattctg atacggcgta gatattgcgc ttgaaaacgc agatatccgc cgcgcatctt
     2881 cgtcgaatag gtcgttacga aaagacggct gatcgccgag aggagaacgg cctgtaacgc
     2941 gcgcaactcc cactcgttcg atgtgacgta gtacagatta tggtgcggat acagctctcc
     3001 ctgttcgtag acgatgtgcg cctcgccctt gatgtcggga atcaggagct tcggtctcca
     3061 cgtcagcgac ggtgtgatgc ggtcgatcgt gcgataccag ttgtccgggg atttttgcgc
     3121 gcagtgacgg cgagcgatca cgtcgcgtct ctcctcaaga taacggccca aacgggggta
     3181 tgccttcaaa tcgaccaggc cgccgccgtc cgcgaacgga ttgacgatcc ctcggccgag
     3241 ccaacggacc tcgccggaga cgatatcttt cgtcgtaacc aaaggaagtt tcctgtccgg
     3301 ttctacgtcg agagcgtcgt acttgccaat gaacgccttg tcggctcctg tcgccactcc
     3361 gatgccgacg gagcatccgg tttcctcaag agaagggaaa ttctcttcca gacgtcggat
     3421 gagatccgtc cggtccgacg
//
LOCUS       DAOTIL010000198         3435 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminobacteriaceae bacterium isolate AMR_MDS_3026
            NODE_32572_length_3435_cov_4.082840, whole genome shotgun sequence.
ACCESSION   DAOTIL010000198 DAOTIL010000000
VERSION     DAOTIL010000198.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868050
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminobacteriaceae bacterium (wastewater metagenome)
  ORGANISM  Aminobacteriaceae bacterium
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae.
REFERENCE   1  (bases 1 to 3435)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3435)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:19:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,224
            CDSs (total)                      :: 3,198
            Genes (coding)                    :: 3,184
            CDSs (with protein)               :: 3,184
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3435
                     /organism="Aminobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_32572_length_3435_cov_4.082840"
                     /isolate="AMR_MDS_3026"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:3034609"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2666)
                     /gene="uvrA"
                     /locus_tag="P5201_05290"
     CDS             complement(<1..2666)
                     /gene="uvrA"
                     /locus_tag="P5201_05290"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009201113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HRV97981.1"
                     /translation="MSQWIHIKGAREHNLKNVDVDLPREQLIVITGPSGSGKSSLAFD
                     TLYAEGQRRYVESLSAYARQFLGVQKKPDVDDISGLSPAISIEQKGTSHNPRSIVGTV
                     TEVYDYLRLLFGRVGTPYCPSCGKPVLRHTLDEIINYLLQDFSEKRLEILAPLVRGKK
                     GEFRNLFAQTREKGFLRVRVDGAVLWLEEEISLDKNKRHSIEAVIDRLRIREDRRSRI
                     AEAIETAFSLSDGYVLLLDEEGNERVLTEKFACADCELTFPEIEPRLFSFNTPYGACP
                     DCSGLGSHQYFAEELAVDPGRTITDGAILPWKKKHYMLTKLEKFAKHNDWDLSSPYGE
                     LPEHVRSFILYGTDERIPLTFRENGEEKSYMGRYEGLLNWLESRWKETESEGVIEELE
                     TYRADDLCQSCKGLRLRPEALYVRLGGYGIGDLISMPVDSLRQTLETISFTSSEEHVV
                     HQVLLEIKKRLDFLSDVGVGYLSLLRRADTLSGGESQRIRLATQIGSKLSGVLYVLDE
                     PTIGLHSRDTEKLVRTLEAIRDLGNTVVVVEHDRETMTAADSIIEMGPGAGEGGGSIV
                     FSGSYEDALHSTHLTGPYLRGERTAVIGEGRRTPKGWLTVKGASHNNLQGIDVSFPTG
                     VFVSVTGVSGSGKSSMVHDVLYKGLRRLLDRDFRERAGNHLTIEGSEQFRNVILVDQS
                     PIGRTPRSNPATYTGLFTLIRELFSGLPESRLRGFAPGRFSFNVKGGRCEACGGGGVT
                     KVSMLFLPDVYVPCEVCGGKRYNHETLEVKFKGKSIADVLDMTVDEAYAFFRDIPRIA
                     SKLQLIAEAGLGYIRLGQSALTLSGGEAQRVKLSKELSKRFSGPTLYLLDEPTTGLFY
                     TDVEKLLHIINKIVDNGNTVILIEHNLDVLLSS"
     gene            complement(2605..>3435)
                     /locus_tag="P5201_05295"
     CDS             complement(2605..>3435)
                     /locus_tag="P5201_05295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006301135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helicase-related protein"
                     /protein_id="HRV97982.1"
                     /translation="MQVSGKMVEQVIRPTGVVDPEVEILPAKGQVDDLISRLRDIVRN
                     GQRALVTTLTKKSSEDLAEYLAELRLKVKYIHSELNAFERAELIRDLRTGDISVLVGI
                     NLLREGMDLPEVALVAILDADREGFLRSYRSLIQMMGRAARNICGKVILYADEITESI
                     RKSVDETVRRRRLQMKYNEENNITPITIQKEIVHLLPEELLEDQPTGKGRGGAETFKE
                     LSVPDMEKMMWQAVERLDFEKAARIRDALASLEGNEWNRVAMDTHKRSTRTQSKKRRR
                     "
BASE COUNT          763 a          915 c          889 g          868 t
ORIGIN      
        1 gaggagagca gaacgtccag gttatgctcg atcaaaatga cagtgttgcc gttgtcgact
       61 attttattga tgatgtggag cagtttctca acatctgtgt aaaagagacc tgtagtcggt
      121 tcgtcgagga ggtaaagtgt agggccgctg aagcgtttgc tcaactcttt ggagagtttg
      181 actctctgcg cttctcctcc gctcagagtg agtgcggact ggccaaggcg gatatacccc
      241 aagccggcct ctgcgatcaa ctgaagtttg gaagcgatcc gtggaatatc acgaaagaaa
      301 gcatacgctt catctacagt catatcaagg acatcggcaa tggattttcc tttgaatttt
      361 acttcaaggg tctcgtgatt atatctcttc cccccgcaca cttcacacgg cacgtaaacg
      421 tcggggagga agagcattga aaccttggtg actccgccgc cgccgcatgc ttcgcatctt
      481 ccgcccttga cgttgaaact gaagcgcccc ggggcaaatc cgcgcaggcg cgattcggga
      541 agccccgaaa agagctcccg tatgagtgtg aaaagtcccg tatacgttgc agggttagaa
      601 cgtggggtcc gccctatagg actttggtcg acaaggatga cgttcctgaa ctgttcgctc
      661 ccttcgatcg tcagatgatt gccggccctt tcccggaagt ccctgtccaa aagccttctg
      721 agacctttgt agagtacgtc atgtaccatg ctgctctttc ccgagccgga gactccggtg
      781 actgagacaa agacacctgt cgggaacgaa acgtcgattc cctgtagatt gttgtgtgat
      841 gcgcccttta ctgtcagcca gcctttggga gtccgtctgc cctcccctat cacagcggtt
      901 cgctcgcccc gaaggtaagg gcctgtcaga tgtgttgaat gcaaggcgtc ttcatagctt
      961 ccagagaaga cgatactgcc gcccccttct ccggcgccgg gccccatttc aatgatcgaa
     1021 tcggccgccg tcatcgtctc tctgtcgtgt tcaacgacga cgacagtatt gccaagatca
     1081 cggatggctt caagtgttct gacgagtttt tccgtgtccc gtgagtgaag accaattgtc
     1141 ggttcgtcaa gaacgtagag aacgccgctc aacttcgagc ctatctgagt ggcaaggcgg
     1201 attctctggc tctctccccc gctgagggta tcggcgcggc gtagcagaga aagatagccc
     1261 actccaacat cggagaggaa gtcgaggcgt ttttttattt ccagcaatac ttggtgtacg
     1321 acatgttcct cggaagacgt gaaggagatt gtttccagcg tttggcggag agagtcgaca
     1381 ggcattgata taaggtcgcc gataccgtat cctccaaggc ggacgtaaag agcctcgggg
     1441 cgcagcctga gccctttgca ggattggcaa agatcatctg cgcggtatgt ctccagctct
     1501 tcgattacgc cttcggattc tgtttccttc cagcgggatt caagccagtt cagcagccct
     1561 tcgtaacggc ccatgtagct tttttcttcg ccattctctc tgaacgtcaa agggatacgc
     1621 tcatcggttc cgtagagaat aaacgaccga acgtgttcgg gcaattcccc gtagggcgac
     1681 gaaagatccc agtcgttatg tttcgcaaac ttctccagtt ttgtgagcat atagtgtttt
     1741 ttcttccatg gcaaaatggc tccgtcggtg atagtccttc ctggatcaac tgccagttcc
     1801 tcggcaaaat actgatggct ccccaggccg gaacagtcgg ggcaggctcc gtagggcgta
     1861 ttgaaagaga aaagtctcgg ttctatctcc ggaaaggtaa gttcgcagtc agcgcaggca
     1921 aatttctccg tgagaactct ttcgttcccc tcttcgtcga ggagaagaac gtatccgtcg
     1981 cttaaggaga atgcagtttc aatggcttca gctatccgtg aacggcggtc ctcacgtata
     2041 cgaagacggt cgatgacggc ctctatagag tgtcgcttgt tcttatcgag cgaaatttct
     2101 tcttcaagcc agagcacagc gccgtcgaca cgaacacgaa gaaatccctt ctcccgcgtc
     2161 tgggcgaaga ggttgcgaaa ctcgcctttt ttccctcgaa caagcggggc aaggatttcc
     2221 aaacgtttct cggagaagtc ctgcaggaga tagttaataa tctcgtcaag agtatgacga
     2281 agaacaggct tcccgcacga agggcaatac ggcgtcccta ctctgccgaa gagaagccgg
     2341 agatagtcgt acacttccgt aaccgttcct acgatcgaac gaggattgtg cgacgtccct
     2401 ttctgttcta ttgatatggc gggagaaagt cccgaaatgt catcaacgtc gggctttttt
     2461 tgtactccaa gaaactgtct cgcgtatgct gaaagagact cgacatatct tcgttgccct
     2521 tctgcataga gcgtatcgaa ggcaagcgac gattttccgg aaccggacgg tcccgttatg
     2581 acgatcagct gctcgcgggg cagatcaacg tctacgtttt ttagattgtg ttctcgtgct
     2641 ccttttatgt gtatccattg cgacacggtt ccattcattc ccttcaagcg aagcaagggc
     2701 gtccctgatg cgggccgcct tctcaaaatc aagccgttca acggcctgcc acatcatctt
     2761 ttccatgtcc ggtaccgaga gttctttgaa cgtttctgct ccgccacggc ctttccccgt
     2821 gggctgatcc tcaaggagct cttccgggag aagatgtaca atctcttttt gtattgtgat
     2881 aggtgtaata ttgttttctt cattatactt catctgcagt ctgcgccttc gaactgtttc
     2941 atccaccgat ttcctgatac tctcagtgat ctcgtcagca tagagaataa ccttgccgca
     3001 gatgtttcga gccgcccgcc ccatcatctg aatgagcgag cgataggatc tgagaaagcc
     3061 ttccctgtct gcgtcgagaa tggcaacaag cgcaacctcg ggaaggtcca taccttccct
     3121 caggagatta atgccaacca gaacggagat atcgcctgtg cggagatctc gaatgagttc
     3181 cgcgcgctcg aaggcattca gttccgaatg tatgtacttt accttgaggc gaagctccgc
     3241 aaggtattcc gcgaggtctt ctgaggactt cttcgtgagc gtggtgacaa gagcccgctg
     3301 tccgttgcgg acgatatcgc gcagacggga tatgaggtcg tcaacctgcc ccttggcggg
     3361 aagaatttct acttcgggat caacgacgcc cgtggggcgt ataacctgct ccaccatctt
     3421 tccggaaacc tgcat
//
LOCUS       DAOTIL010000199         3432 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminobacteriaceae bacterium isolate AMR_MDS_3026
            NODE_32613_length_3432_cov_3.443293, whole genome shotgun sequence.
ACCESSION   DAOTIL010000199 DAOTIL010000000
VERSION     DAOTIL010000199.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868050
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminobacteriaceae bacterium (wastewater metagenome)
  ORGANISM  Aminobacteriaceae bacterium
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae.
REFERENCE   1  (bases 1 to 3432)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3432)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:19:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,224
            CDSs (total)                      :: 3,198
            Genes (coding)                    :: 3,184
            CDSs (with protein)               :: 3,184
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3432
                     /organism="Aminobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_32613_length_3432_cov_3.443293"
                     /isolate="AMR_MDS_3026"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:3034609"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..532
                     /locus_tag="P5201_05300"
     CDS             <1..532
                     /locus_tag="P5201_05300"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRV97983.1"
                     /translation="PFEDMKDPEFVRFAAATLNFPAGRFFYDTLKGVGSLVRYLLLGG
                     VLLLFGGVFFGLFKRFRGAFFSPPSRYTIKEGTEKTERKALKDVFADDERADGGGADE
                     TASFTVEPEMIEDPEISPAETDDATGKEEDLQEEDAVSADEELQRGQSDEESAGTAGK
                     STRQRRRKKRMKKENE"
     gene            525..863
                     /locus_tag="P5201_05305"
     CDS             525..863
                     /locus_tag="P5201_05305"
                     /inference="COORDINATES: protein motif:HMM:NF014019.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal-binding domain-containing protein"
                     /protein_id="HRV97984.1"
                     /translation="MSKKGIPVLTVDVIPGKKCEYLGCVSASCCLSKSLVQDVASNVK
                     NWTVGGELTRYTDMIDDAVNLVTERLGVLAQQMGANAIIGFRLSTTSVSTGAAELIAY
                     GTAVRFPDLN"
     gene            complement(860..2026)
                     /locus_tag="P5201_05310"
     CDS             complement(860..2026)
                     /locus_tag="P5201_05310"
                     /inference="COORDINATES: protein motif:HMM:NF019310.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HRV97985.1"
                     /translation="MDRLILSLMIINFAIHSYSNTFFFLAPYLSTQGYSPASAGVLVS
                     VFYAATTAVRPVGSWITERFGIRGSMIASSLLCLACAAVLHIYQGSFSFFVGLRIVMG
                     IGFSVFMVALTTYQALAVEEAKRGLAFALVAIGSIVPMFTIVPLTDFLIRNNLGRIYL
                     LLPVAASALALMLAIRLNTKQTTGFEETNSTMKWGSYRDLFRETPAWRMLCSCFFFGL
                     CDSSIVFLGSMLLEKGLVPSWFVFAMAGGALFVRTAGRNLFNRYPRTVFAGPSFLLMA
                     ACLIWLSFVDSPAGMLLSGFLYGIGVGYGYPAHLALTADLAPARLRAKASALVHFSMD
                     LSWFLLPLYMGFGSTLLGTLLAFRLFALFCLITAVAVTVMWLPGKGRAGGGAPA"
     gene            complement(2222..2392)
                     /locus_tag="P5201_05315"
     CDS             complement(2222..2392)
                     /locus_tag="P5201_05315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012869128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HRV97986.1"
                     /translation="MAKAVVDKDACVGCETCVGTCPVEAISMVDGKALVDESCIECGS
                     CVSVCPVSAITQ"
     gene            complement(2512..>3432)
                     /locus_tag="P5201_05320"
     CDS             complement(2512..>3432)
                     /locus_tag="P5201_05320"
                     /EC_number="1.1.1.94"
                     /inference="COORDINATES: protein motif:HMM:NF000940.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009331 - glycerol-3-phosphate
                     dehydrogenase complex [Evidence IEA];
                     GO_function: GO:0047952 - glycerol-3-phosphate
                     dehydrogenase [NAD(P)+] activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0046167 - glycerol-3-phosphate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent glycerol-3-phosphate
                     dehydrogenase"
                     /protein_id="HRV97987.1"
                     /translation="QARAINATSHNPDYLQAIELPEAISSTSDPEEAALFSQHWILAL
                     PTQTVRGFLPALAPFCGKETEVCNVAKGIEIGTGARISAIVSETLPEVRYSVLSGPSF
                     AGEVAQGLPTALTAASRHETSALLWQSALNTQRLRIYTSSDVVGTETGGAVKNIMAIA
                     SGMASALGLGENARAALVTRGLAEIMRLGEALGAHPLTLAGLAGMGDLVLTCYSTQSR
                     NFRLGLALGRGLSLDEAKLEVGQVAEGAFTVRAVAESALALSVELPVSQGVHRLLYEG
                     SSPQDELERLIARDPKAEYPPAMLWGSGRG"
BASE COUNT          759 a          971 c         1097 g          605 t
ORIGIN      
        1 ccccttcgag gacatgaagg atccggagtt tgtccgtttc gccgcagcca cactcaactt
       61 tccggcaggg cgatttttct acgacaccct caagggggtg ggcagccttg tccgctatct
      121 tcttctcggc ggcgtgctgc tgctcttcgg cggcgtcttc ttcgggcttt ttaaacgatt
      181 ccgcggcgcg ttcttctctc cgccttcacg ctatacgatc aaagagggga cggaaaaaac
      241 tgaacggaag gcgctgaaag atgtttttgc cgatgacgag agagcggatg gaggcggagc
      301 cgacgaaacg gcttcattta cagtggaacc agaaatgatc gaggatccgg aaatttcccc
      361 cgcagaaacc gatgatgcaa cggggaaaga ggaggatctg caggaagagg acgccgtatc
      421 cgcggacgag gagcttcaac gcgggcaatc cgacgaggag agcgcgggca cggctggaaa
      481 atcaacacga cagaggagac gcaagaaaag gatgaaaaaa gagaatgagt aaaaagggaa
      541 ttccggtcct cacggtcgac gtgattccgg gcaagaagtg cgagtatctg gggtgcgtgt
      601 cggcctcctg ctgtctttcg aagtcgctgg tgcaggacgt ggcgtcgaac gtgaagaact
      661 ggactgtcgg aggggaactg acccggtaca ccgatatgat cgacgacgcg gtcaaccttg
      721 tgacggagcg cctcggcgtg ctggcgcagc agatgggagc gaacgcgatc atcggcttcc
      781 ggctctcgac gacgagcgtc tccaccggcg cggcggagct tatcgcctac ggaacggcgg
      841 tgcgcttccc cgacctgaac taagcgggcg ccccgccgcc ggcgcgccct tttcccggca
      901 gccacatgac ggtgacggca acggccgtga taaggcaaaa gagtgcgaaa aggcgaaatg
      961 ccaaaagcgt gccgaggagc gtcgagccga accccatgta gaggggaaga aggaaccagc
     1021 tcaggtccat ggaaaagtgg accagcgccg acgccttggc cctgagacga gccggagcga
     1081 gatccgcggt gagcgcaaga tgcgcggggt agccgtaccc gacgccgatt ccgtagagga
     1141 atccggagag cagcatcccg gcaggtgaat cgacgaagga gagccagatg aggcacgctg
     1201 ccatcagcag aaacgagggg ccggcgaaga cggttctcgg gtagcggttg aacaggtttc
     1261 tccctgctgt acggacgaag agcgcccctc ccgccatagc gaagacgaac catgacggga
     1321 caagcccttt ctccagcagc atgctgccga gaaagacaat ggaggagtcg cagaggccga
     1381 agaagaaaca ggagcagagc atcctccacg cgggcgtctc ccggaagagg tcgcgataac
     1441 tcccccactt catggtcgag tttgtctctt cgaagccggt cgtttgcttt gtgttgaggc
     1501 gaatcgcgag cataagagcg agcgccgacg cggcaaccgg aagaagcagg tagattctgc
     1561 cgaggttgtt ccggataaga aagtcggtca gcgggacgat agtgaacatg gggacgatgc
     1621 tgccgatggc gaccagggca aatgcaagtc cccttttcgc ttcttccacg gcgagcgcct
     1681 ggtaggttgt aagcgcgacc atgaagacgc tgaagccgat tcccatgacg atacgaagcc
     1741 cgacgaagaa ggagaacgaa ccctggtaga tgtggagaac cgctgcgcag gcgaggcaga
     1801 gcaaagacga tgcgatcatg cttcccctga tcccgaatcg ctccgtgatc cagcttccca
     1861 cggggcggac cgcggtcgtc gccgcgtaga acacgctcac cagcacgcct gcggatgcgg
     1921 gggagtaccc ctgtgtgctc aggtagggcg ccaggaagaa gaacgtgttg gagtacgagt
     1981 gtatcgcgaa gttgatgatc atcagggaaa ggatgagccg atccatggaa gcctccggaa
     2041 gagagatttt gctgacgctg tttcgcggcg gattgtccgc ctgttcggga gggcgtcgcg
     2101 cggaggttag tgtataccgt aaccgctttt tattcaatct cctgtatcgt aaagaaaacg
     2161 cccctccgcg gaatggaggg gcgtttcgaa aaaacgtgtt cgtaacgaga tcagagactt
     2221 actactgggt gatcgcgctc acgggacaga cggacacgca gcttccgcac tcgatgcagg
     2281 actcgtcaac gagagccttg ccgtcgacca tcgaaatagc ctcgaccggg caggtgccta
     2341 cgcaggtctc gcaacccacg catgcatcct tatcaacaac tgcctttgcc ataaatacat
     2401 tcctcctcag caagggatat tctgacgcta ttgaacgact tggattatac atcactttct
     2461 tacctcgggc aacttttgac gcaactccgt gaataaccgg agatacggag gtcaccccct
     2521 cccgcttccc cagagcatcg cgggcgggta ctccgccttc gggtcgcggg cgatcagccg
     2581 ttccagctca tcctgaggag aagagccttc gtagagaagg cggtgcactc cctgcgagac
     2641 cggaagttcc acggagagcg cgagcgcgct ttcagcgacg gcgcggaccg tgaacgctcc
     2701 ttcggcgacc tgaccgacct caagtttcgc ttcatcgagg gagagacccc ggccgagcgc
     2761 gagcccaagg cggaagttgc gcgactgcgt gctgtagcag gtgaggacga gatcccccat
     2821 tcccgcgagc ccggcgagcg tcagcggatg ggctccgagc gcttcgccga ggcgcatgat
     2881 ttccgcaagt ccgcgggtta ccagcgctgc gcgcgcgttt tctcccaaac caagggcgga
     2941 agccattccg gaggcgatcg ccatgatatt ttttaccgcg ccccccgttt cggtaccgac
     3001 gacgtcggac gacgtgtaga tgcgcagtct ctgggtattc agcgcagatt gccagaggag
     3061 cgcggacgtc tcgtgcctgg aggcggcagt cagtgcggtc ggaaggcctt gcgccacctc
     3121 cccggcgaaa ctcggtcccg aaaggacgga gtagcggact tccggaagcg tctccgaaac
     3181 gatagcgctg attcttgctc ccgtgccgat ctcgatcccc tttgccacgt tgcagacttc
     3241 ggtctctttg ccgcagaagg gggcgagcgc cggaaggaaa ccccgcaccg tttgcgtcgg
     3301 cagcgcgagg atccagtgct gggagaagag cgccgcctcc tcgggatcgg atgtggacga
     3361 gatcgcctcg ggaagctcaa tcgcctggag gtagtccggg ttatgcgagg tcgcgttgat
     3421 cgctcgcgcc tg
//
LOCUS       DAOTIL010000200         3428 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminobacteriaceae bacterium isolate AMR_MDS_3026
            NODE_32665_length_3428_cov_3.567151, whole genome shotgun sequence.
ACCESSION   DAOTIL010000200 DAOTIL010000000
VERSION     DAOTIL010000200.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868050
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminobacteriaceae bacterium (wastewater metagenome)
  ORGANISM  Aminobacteriaceae bacterium
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae.
REFERENCE   1  (bases 1 to 3428)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3428)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:19:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,224
            CDSs (total)                      :: 3,198
            Genes (coding)                    :: 3,184
            CDSs (with protein)               :: 3,184
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3428
                     /organism="Aminobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_32665_length_3428_cov_3.567151"
                     /isolate="AMR_MDS_3026"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:3034609"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            140..1081
                     /gene="arcC"
                     /locus_tag="P5201_05325"
     CDS             140..1081
                     /gene="arcC"
                     /locus_tag="P5201_05325"
                     /EC_number="2.7.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005660127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008804 - carbamate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbamate kinase"
                     /protein_id="HRV97988.1"
                     /translation="MRVLVALGGNAILQRGQKGNASEQRANVQKTVTQIVRMIHAGHE
                     VVVTHGNGPQVGAILIQNELGSASVPAMPMDICGAESQGLIGYMFCQSIQNTLLADGI
                     EGHAPACLVTRVEVDPADPAFTHPTKPVGPFYTEEAAKKRIAETGETWIDDAGRGWRR
                     VVPSPDPKSIVEADVINTLLEKGFTVVASGGGGIPVIRKENGTWEGVEAVIDKDLAGE
                     RLAASVKADLLMILTDVPKVAIHYNTPQQKWLDKLTLSEMESLEKEGHFKAGSMGPKV
                     KAALRFVRNGGQRAVIASLDEALEALEGTKGTQILKG"
     gene            1216..1929
                     /gene="kdsB"
                     /locus_tag="P5201_05330"
     CDS             1216..1929
                     /gene="kdsB"
                     /locus_tag="P5201_05330"
                     /EC_number="2.7.7.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006300503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008690 - 3-deoxy-manno-octulosonate
                     cytidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0009244 - lipopolysaccharide core region
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-manno-octulosonate cytidylyltransferase"
                     /protein_id="HRV97989.1"
                     /translation="MNALAVIPARYASTRLPGKPLLPVAGVPLVLRVLEQVQKCSGVD
                     RIIVATDDQRIADVVENAGAEALLTPPELPSGGDRVAWVSERIPSKIVLNIQVDDPLV
                     GPDMIDPLIAALSDEGTALALLAKRIENEREIESPNIVKMVFDKNQRALYFSRSPIPY
                     PRTSGAAYYKHIGPYAWKREFLLQFSGWNQTPLEKSESLEMLRVLENGYSIRCIPVAR
                     DTIEIDTPEDVAAFEALLA"
     gene            1992..3149
                     /locus_tag="P5201_05335"
     CDS             1992..3149
                     /locus_tag="P5201_05335"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014162716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0030554 - adenyl nucleotide binding
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase"
                     /protein_id="HRV97990.1"
                     /translation="MQFNMFLAGRIAFGPGVSSLTGEKAFALGGKRIFLVTDRTMEKL
                     GTAEKIVSYINAAGLSVIPFYDVEPEPSVETVDAATALAKREKCDLVVGLGGGSCLDV
                     AKATSVLLTNEGSAARYQGLGLVKNAGVPKIMIPTTAGTGSEVTFTSVLIRKSDGVKG
                     GINGDQLYPECSLLDPELTVSMPSSVTAATGMDALAHALEAYTSKQASPFSDMFAEQA
                     ISRIGRYLRVAVYNGTDIHARSEMMLAALYGGIALANAGVTACHALAYPLGSMFGIGH
                     GQANALLLPYVAQFNAMALPEKFRAAAELLGGSYEEESLWDAASMCSALLHQLVADIN
                     LPSTFPELDPRITEDHFATMAEKAMGVARPMANNPRTMNTDLCMEIYKEAM"
     gene            3151..>3428
                     /locus_tag="P5201_05340"
     CDS             3151..>3428
                     /locus_tag="P5201_05340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009201628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegT/DnrJ/EryC1/StrS family aminotransferase"
                     /protein_id="HRV97991.1"
                     /translation="MPGSELFDHLEIEAVADVLKRKVIHRYAYHEVRDGNYRVDEFEA
                     KVAELSGAKHCLAVSSGTAALYVPFKAMGICPGDEIITSSFTFIATVEA"
BASE COUNT          818 a          968 c          954 g          688 t
ORIGIN      
        1 gatggaacaa aatactatac ggaggacatt cacaaagcgg ctttcgtgct tccgccgttt
       61 gttaaagact tgctgaaata aggcgtatac tctataatat agaagtcaac tacgacattc
      121 aggagggaat tttgcagaga tgagagtatt ggtcgccctt gggggcaatg ccattcttca
      181 aagagggcaa aagggaaacg cctcggaaca gcgtgcgaac gtccaaaaga ccgtgacgca
      241 aattgtcaga atgatccatg ccgggcacga agtggtcgtg acgcacggaa acggccctca
      301 ggtcggagca attctcatac agaacgaact cgggagcgcc tctgttcccg cgatgcccat
      361 ggatatatgc ggcgccgaga gccaggggct catcggatac atgttctgcc agagcataca
      421 gaataccctg cttgcggacg gcatcgaagg acacgcgcct gcttgccttg ttacccgagt
      481 cgaagtggat cctgcggatc cggcgtttac ccatcccaca aagccggtgg gtcccttcta
      541 tactgaagag gccgctaaga agcgcattgc cgagaccgga gagacatgga tcgacgatgc
      601 gggacgagga tggcgccggg ttgtcccctc cccggatccc aagtcgatcg tcgaagcgga
      661 cgtcatcaac acgctccttg aaaaaggatt cacagtagtt gcctccggcg gcggcggtat
      721 tcccgtcatc cggaaggaaa acggaacatg ggagggtgtg gaagccgtca tcgacaagga
      781 tcttgcaggc gaacgccttg ccgcttctgt gaaggcggat ctgctgatga ttctcaccga
      841 tgttcccaag gtagccattc actacaatac accgcagcag aagtggctcg acaagctgac
      901 gctctccgag atggagtctc tcgaaaaaga agggcatttc aaggccggtt ccatggggcc
      961 aaaagtaaaa gctgcactcc ggttcgtccg taacggcgga cagcgcgcgg tgatcgcaag
     1021 cctcgatgaa gctctggaag ccctcgaagg aaccaagggt acccagattc ttaaaggtta
     1081 gttttttatc ttcgcgatat aaacctccct cactcaagag ggaggttttt tgttgatgcg
     1141 aaaaagagtc tatggaggtg ataataaatg atgtagaatt tacttccggt ttttttccgg
     1201 caaggagatg gatttatgaa cgctcttgca gtcattccgg cccggtacgc gagcactcgt
     1261 ctcccgggga agccccttct tccagtcgca ggcgtaccgc tcgtcctcag agttctggaa
     1321 caggttcaaa agtgcagcgg cgtcgaccgc atcatcgttg ccactgacga ccaacgaatt
     1381 gccgatgtcg tcgaaaacgc cggagcggaa gctctgctca ctccgcctga gctgccaagc
     1441 ggaggcgacc gtgtcgcatg ggtctccgaa cgaattcctt caaagatcgt tttgaatatc
     1501 caggtcgatg atccgcttgt cggcccggac atgatcgacc cgctgattgc ggcgctctcg
     1561 gacgaaggca cggcgcttgc actgcttgcc aagcgaatag agaacgaacg ggagatagaa
     1621 tcgccgaaca tagtaaaaat ggtcttcgac aagaaccaga gagcgctcta cttcagcagg
     1681 tctcccatcc cctacccccg cacctccgga gcggcatact acaaacatat cggcccgtac
     1741 gcctggaaac gagaattttt gcttcaattc tccggctgga accagactcc gcttgaaaaa
     1801 tcagaaagcc ttgagatgct gcgggttctg gaaaacgggt actcaatccg ctgtattccc
     1861 gttgcacggg acactataga aatcgacact cctgaggacg ttgcggcgtt tgaagcgctg
     1921 ctcgcctgac ggaatgatcg acgaaccaca gagaccgcgg caacagccgc aaggaaatta
     1981 gaggtggaaa gatgcaattc aacatgtttt tggccggacg aatcgcgttc ggccccggag
     2041 tctcttccct cacgggagag aaggcgttcg ctcttggggg caaaagaatc tttcttgtca
     2101 ccgacagaac catggagaag cttgggacgg cggaaaagat cgtttcatat atcaacgccg
     2161 ctgggttgtc cgtaatcccc ttttacgacg tggagccgga accgtccgta gaaaccgtcg
     2221 acgccgcaac cgcgcttgca aaaagagaaa agtgcgatct cgtcgtcggc ctcggaggag
     2281 gaagctgcct cgacgtggcg aaggcaacga gcgtcctcct gacaaacgaa ggctccgcag
     2341 cccgctacca gggtctcgga cttgtcaaga acgcaggcgt tccgaagatc atgatcccca
     2401 ccaccgccgg cacagggtcg gaagtaactt tcacctccgt cctgatacgg aagagcgacg
     2461 gcgtaaaggg agggatcaac ggagaccagc tctaccctga gtgcagcctc cttgaccccg
     2521 aactgactgt ctccatgccg tcctccgtca ccgccgctac aggcatggac gcgcttgccc
     2581 atgccctcga agcgtacacg agcaaacagg cttcgccgtt cagcgacatg tttgccgagc
     2641 aggccatctc ccgtatcggc cgctacctcc gggtcgctgt ttataacggg acggatatac
     2701 acgcccgaag cgagatgatg ctcgcagctc tctacggtgg aatagcgctc gccaacgccg
     2761 gtgtcacagc gtgccacgcg ctcgcctatc ctctcggcag catgttcggc atcgggcacg
     2821 gacaggcgaa tgcgctcctt ctcccctacg tggcgcagtt caacgcgatg gcgctgcctg
     2881 agaagttcag agcagccgca gagctcctgg gcggttccta cgaagaggag agtctctggg
     2941 atgcggcttc tatgtgctcc gcgcttctcc accagcttgt cgccgacatc aaccttccct
     3001 caacttttcc cgaactcgat cctcgtatca ctgaagacca cttcgcgacg atggccgaga
     3061 aggcgatggg cgtcgcgcgt ccgatggcaa acaacccccg gacgatgaat accgatcttt
     3121 gcatggaaat ctataaggag gcgatgtaac atgccgggtt ccgaactctt cgatcatcta
     3181 gaaatagagg ccgtcgccga cgtcctcaag cgcaaagtca ttcaccgcta cgcttatcat
     3241 gaggtacggg acggaaacta cagggtggac gaattcgagg caaaagtcgc ggaactctcc
     3301 ggagcgaagc actgtctcgc cgtgagcagc ggtacggctg cgctttacgt tccgttcaag
     3361 gcgatgggga tctgccccgg agacgagatc atcacatcct cgttcacttt tatcgccacg
     3421 gttgaagc
//
LOCUS       DAOTIL010000201         3425 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminobacteriaceae bacterium isolate AMR_MDS_3026
            NODE_32700_length_3425_cov_3.492878, whole genome shotgun sequence.
ACCESSION   DAOTIL010000201 DAOTIL010000000
VERSION     DAOTIL010000201.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868050
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminobacteriaceae bacterium (wastewater metagenome)
  ORGANISM  Aminobacteriaceae bacterium
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae.
REFERENCE   1  (bases 1 to 3425)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3425)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:19:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,224
            CDSs (total)                      :: 3,198
            Genes (coding)                    :: 3,184
            CDSs (with protein)               :: 3,184
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3425
                     /organism="Aminobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_32700_length_3425_cov_3.492878"
                     /isolate="AMR_MDS_3026"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:3034609"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..235)
                     /locus_tag="P5201_05345"
     CDS             complement(<1..235)
                     /locus_tag="P5201_05345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009200884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3798 domain-containing protein"
                     /protein_id="HRV97992.1"
                     /translation="MKKALGIMLVFVMVVSLVASVVPAFAGAGKVAIVTNTVSQNEEE
                     YRSAQEMVAKYGDRVVHVTWPDNFMAEQEQMVSV"
     gene            522..1631
                     /locus_tag="P5201_05350"
     CDS             522..1631
                     /locus_tag="P5201_05350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008710902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Xaa-Pro peptidase family protein"
                     /protein_id="HRV97993.1"
                     /translation="MINQKRLKKLADELHKLGLDAIYLGPSTDLEYISGLDTHPDERV
                     RGLMVSKDARCFSMTPLLYKEEMVKAFGDVPFYAEWNDHEGFTGAFRRGCEHLGLVGA
                     KIAFNDGVRAIDMLAIRDAMELQMCNGGKVLDPLRSRKDEEELGYMREASRMVDEVVR
                     KLHGFIRPGMTERQIAKQIPEFFEEAGSNGISFSPIVASGPNGSMPHYGGDQRVIEKN
                     DVIILDLGGRHMSYCSDTTRTFFVGTPTEEQRKVYEIVRQAQAAGEAAVQPGVTGQEV
                     DRAARKVIVDAGYGDYFFNRVGHGIGLAVHESPYIIEGNDKPLEPGNVFSVEPGIYLP
                     GKFGIRIENIVAVRPDGTGEALNHFPRDIESVCIK"
     gene            complement(1713..2723)
                     /gene="dctP"
                     /locus_tag="P5201_05355"
     CDS             complement(1713..2723)
                     /gene="dctP"
                     /locus_tag="P5201_05355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006300343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter substrate-binding protein DctP"
                     /protein_id="HRV97994.1"
                     /translation="MRKVTRYFLLVPALVVLACLVRPAAAAPETVLRFAGQFPAEHTS
                     TKLMQQLADEVKEKTGGRIEIRLHPDNELGDYTLIFEELIRGSIDMAAITVPSQFDPR
                     LELCNINGFVRTFDEARTVFAPDGWVFKKMDELNSALGVKLLGFQFEGFIGIGSTKPV
                     NEPLNPEVKKGILQRIPNMEVFKLGAEAMGYRTITIPWDEVHGSLQNGFAEGVYGMTP
                     AAAYTILKDVIKYWYDLRHSVENLNYMMSLKTWEKLSEEDRGIIQNACAKISAVSVYL
                     AEKDQEKYLQSMRDAGIEVHTYTPEELAPLFVKVSATWDKLAERFSQELIDEFKKEFA
                     RQ"
     gene            3143..>3425
                     /locus_tag="P5201_05360"
     CDS             3143..>3425
                     /locus_tag="P5201_05360"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="rubrerythrin family protein"
                     /protein_id="HRV97995.1"
                     /translation="MKHQPSPSILQRILQFQRDELTSSILYEYIAGRQKDEGNRTALL
                     EIAKAERSHYTTWKGYTGQELRPNMLKIFFYKIIHMLLGDTFTIKLFEIG"
BASE COUNT          795 a         1008 c          925 g          697 t
ORIGIN      
        1 cgacgctgac catctgctcc tgctccgcca tgaagttgtc gggccacgta acgtggacaa
       61 ctctgtctcc gtacttcgcg accatttcct gcgcggaacg atactcttcc tcgttctgag
      121 aaacagtatt cgttactatc gcaaccttac cggcccccgc aaacgccggg acaaccgaag
      181 caacaaggct gacgaccatg acaaaaacca acataatgcc aagtgccttc ttcattcagt
      241 accctcctat ccaattgtaa gttcaaaacc tttttctaag aaaagatacc gcccagcgcg
      301 ccccttgtca agcgctaact tttctttgga ataatgacct tataacaatt tcatcaaatc
      361 aacacagtaa atatttgaac caaggtgcct aaaagagata ataactcacc ctagtttcgc
      421 atgtcttgct ctttttcgga aagttatttt ggacaatggc cttttcaggc ggtagaatag
      481 agcgtagcgg ttgtttcgca actctttccg gagggatgtt catgatcaat caaaagaggt
      541 taaaaaagct tgccgatgag ttacacaagc tgggtttgga cgcaatctac cttggccctt
      601 caacggacct ggagtacatc agcggcctgg atacgcaccc cgacgaacgg gtcaggggac
      661 tgatggtctc gaaggacgcc cgttgcttct cgatgacccc cctgctctac aaggaggaga
      721 tggtgaaggc gttcggcgac gttccctttt acgccgagtg gaacgaccac gaaggcttca
      781 ccggcgcgtt ccggcggggg tgcgaacacc tcgggctcgt cggcgcaaag atcgcgttca
      841 acgacggcgt gcgggcgatc gatatgctcg cgatcagaga cgcgatggag ctgcagatgt
      901 gcaacggcgg caaggtgctc gacccgctcc gctcccgcaa ggacgaggag gagcttgggt
      961 acatgaggga ggcgtcccgc atggtcgacg aggttgtgcg gaagctgcac ggctttatac
     1021 gcccgggaat gaccgaacga cagatcgcca aacaaatccc cgagttcttc gaggaggcgg
     1081 gctccaacgg catctccttc agccctatcg tggccagcgg ccccaacggc tccatgccgc
     1141 actacggcgg agaccagcgg gtgatcgaga agaacgacgt aatcatcctc gacctcggcg
     1201 gccgccatat gagctactgt tcggacacga cgcgcacgtt ctttgtcggc acgccgaccg
     1261 aggagcagag gaaagtgtat gagatcgtcc ggcaggccca ggccgccgga gaagcggcgg
     1321 tacagcccgg agtcaccggc caggaggtcg acagggcggc gcgcaaggtc atcgtcgacg
     1381 cggggtacgg ggactacttc ttcaaccgcg tggggcacgg gatcggcctc gcggtgcacg
     1441 aatcccccta catcatcgag gggaacgaca agccgctcga gccggggaac gtcttcagcg
     1501 tcgagccggg catctacctg ccggggaagt tcggcatcag gatcgagaac atcgtcgccg
     1561 tgcgccccga cggaacgggc gaagcgctca accacttccc aagagacatc gagtcggtct
     1621 gcatcaaata gtcgcagaac caacggagaa caggaaaaag gggcgcccgg aaagaggacg
     1681 ccccttttca tatcctggcg gaagcccgaa cgctactgcc gggcgaactc cttcttgaac
     1741 tcgtcgatca gctcctgcga gaagcgctcg gcgagcttgt cccacgtcgc ggacaccttg
     1801 acgaacagcg gcgccagctc ctcgggcgta tacgtgtgca cttctatacc ggcgtcgcgc
     1861 atactctgaa ggtatttctc ctgatccttc tcggcgaggt agacgctgac tgcggagatt
     1921 ttcgcgcagg cgttctggat gattccgcga tcctcctcgg agagcttctc ccaggtcttc
     1981 aggctcatca tgtagttcag attctccacc gagtggcgca ggtcgtacca gtacttgatc
     2041 acgtccttaa ggatggtgta cgccgccgcg ggcgtcattc cgtacactcc ctcggcaaag
     2101 ccgttctgga gcgagccgtg cacctcgtcc cacgggatgg tgatcgtccg gtagcccatc
     2161 gcctcggcgc cgagcttgaa gacctccatg ttcggaatgc gctgcaggat tccttttttg
     2221 acctcgggat tcaacggctc gttcacgggc ttggtgcttc cgatgccgat gaacccttcg
     2281 aactggaagc cgaggagttt cacgccgagc gcggagttca actcgtccat cttcttgaat
     2341 acccatccgt cgggagcgaa gaccgttctg gcctcgtcga acgtccggac gaagccgttg
     2401 atgttgcaga gctccagccg cggatcgaac tggctgggaa cggtgatcgc cgccatatcg
     2461 atggaaccgc ggatcagctc ctcaaaaatg agcgtatagt cgccaagttc gttgtcgggg
     2521 tggagcctga tctctattcg cccgccggtc ttctccttca cctcgtcggc cagctgctgc
     2581 atcagcttcg tcgacgtgtg ctccgcagga aactgtcccg cgaaacggag caccgtctcc
     2641 ggggcggccg cggcggggcg gacaaggcat gcaagaacaa caagcgcggg aacaagcaag
     2701 aaataccgag taacctttcg cattcaacgc actcctctcg gaataaaacg atctgttccg
     2761 gttgcagtca accggcccgg cgtccttgga gcattcaagc tccggcgcca ttcccaccct
     2821 tctccggact gagacagacc gcgttcaccc cctcaagagt tccgtatcgg acgttttccc
     2881 gtctctccgg atgtttcaag aaaacccggc tgcgtatttt caggagactt catttcactt
     2941 cgcaagacga tattttcctc attgtagcag cgtgtacaac cgaagtccac acccgttcgg
     3001 caaaaatgtt cccaaattca caacgtcgac agccgagcgc caaagacgtt tcgacataaa
     3061 tttcacatgg tataataaaa agttacatac tatcgcaaaa tcctcaagct ctctgcagaa
     3121 aatgtgaaag gcaggcaaaa atatgaaaca tcaaccgagt ccatccatac ttcaacggat
     3181 cctacagttc cagcgcgacg aactgacctc cagcatcctg tacgagtata tcgccggacg
     3241 tcagaaggac gagggtaaca ggaccgccct gctggaaata gcgaaggcgg aacggagtca
     3301 ttacacaacg tggaaagggt acacgggaca ggaactccgc ccgaacatgc tcaaaatttt
     3361 cttctataaa attatacaca tgctcctggg cgacaccttc accatcaagc tttttgaaat
     3421 tggag
//
LOCUS       DAOTIL010000202         3419 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminobacteriaceae bacterium isolate AMR_MDS_3026
            NODE_32772_length_3419_cov_4.669144, whole genome shotgun sequence.
ACCESSION   DAOTIL010000202 DAOTIL010000000
VERSION     DAOTIL010000202.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868050
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminobacteriaceae bacterium (wastewater metagenome)
  ORGANISM  Aminobacteriaceae bacterium
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae.
REFERENCE   1  (bases 1 to 3419)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3419)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:19:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,224
            CDSs (total)                      :: 3,198
            Genes (coding)                    :: 3,184
            CDSs (with protein)               :: 3,184
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3419
                     /organism="Aminobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_32772_length_3419_cov_4.669144"
                     /isolate="AMR_MDS_3026"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:3034609"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..283)
                     /locus_tag="P5201_05365"
     CDS             complement(<1..283)
                     /locus_tag="P5201_05365"
                     /inference="COORDINATES: protein motif:HMM:NF028309.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATPase subunit subunit G family protein"
                     /protein_id="HRV97996.1"
                     /translation="MSLADIKKKIENDARDEAGRILEKARENVEAIKKNAADEIKKME
                     NGYSDRFKKEQPEILRRREIVAGLDVKKIELGVRQQAITDAFQSALKTLA"
     gene            complement(305..784)
                     /locus_tag="P5201_05370"
     CDS             complement(305..784)
                     /locus_tag="P5201_05370"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008522024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit K"
                     /protein_id="HRV97997.1"
                     /translation="MDLLGIMLAILGAAMAAGWAGAGSAIGVGIAGEAGAGVMTEDPG
                     KFGLVLLLQALPGTQGIYGLLIAFFAILKVGLLGGGAGVTVNVWQGLGIFFACLPIAV
                     GGFFSAIAQGKTSAACIQMIAKRPEETGKAVILPAMVETYAVLALLMSIILLNGITL"
     gene            complement(830..2842)
                     /locus_tag="P5201_05375"
     CDS             complement(830..2842)
                     /locus_tag="P5201_05375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013048201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit I"
                     /protein_id="HRV97998.1"
                     /translation="MAVARIKKAELYVHKADLDNVLHALQKTGSFEVVPFGSESVSES
                     TAPVHTDLSRVEGLLSEARFLLRFLEPYFKDNVSSLARTMGERETISLHDGKTLEDRT
                     DLMSLSASVRSLERRLMEIRSELTQIASTETLLNALSDFPYPFELLTSGTERVRGILG
                     TMPVSKIPAWREETEALLLSMGEVFTSTQGEKDTEGWIAVFFDEQKTAQITDLLARNS
                     FSRVDVPLSLKKTPEEERLALSDRKVALASEEERVLAEIAGAAEQWVPSVRILSDYWT
                     VLRDRYQAISSGSSTEQVIMLRGWVPASAVQRLTKAITPFGLSVELFLFDPKPEDSPP
                     SHIVNSAWCFPFENLTRLYGAPKYGEIDPTPLLAPFFFIFFGMCLGDAGYGAIMIGFF
                     LWFLRKYKKMPESFKGFFKLFVLSGVSTIVVGALTGSWFGDLVDAFSIFAFLRPLKNF
                     MLVLNPMENPMIFLAISLALGILQLFFGLFIAFYDALRKGNYMGAFADTGGWIVFLTG
                     LLLWGGVASGFISAQTSFLAKTLTFLGAAVLIATQGREKEGIIGKAVSGLLSLYSVTS
                     YLGDVLSYSRLLALGLATSAVGVIINMLAGLAGDVPYIGWIIALLLLIGGHIFSIAVN
                     VLGAFVHSLRLQYVEFFSKFYTGGGTVFSPLTYKTAFVAIKNEPEE"
     gene            complement(2823..3155)
                     /locus_tag="P5201_05380"
     CDS             complement(2823..3155)
                     /locus_tag="P5201_05380"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="cell envelope biogenesis protein TolA"
                     /protein_id="HRV97999.1"
                     /translation="MAANLAEEIKEVEARSKDIVKDAKNGAAQMINEAQNEVERRTKE
                     AKQKAFRLFKENTASVEREAEEKAKQSVEAGQKKSEQLAKQYGQKVNSAAQWISEEVI
                     SRYGSSEN"
BASE COUNT          905 a          864 c          792 g          858 t
ORIGIN      
        1 acgcgagcgt cttcagtgct gattgaaaag catccgttat agcctgctgc ctgactccaa
       61 gttcgatttt tttcacatca agaccggcga cgatctctcg tcttctgaga atctctggct
      121 gttctttttt aaaacggtcg ctatagccgt tctccatttt tttaatctcg tcagcagcgt
      181 ttttcttaat cgcctcgacg ttttctctgg ctttttccag aattcttccc gcctcatcac
      241 gcgcgtcatt ttctattttt ttctttatat ctgccaagga cattcagcat tcctccttga
      301 caatctacag agtgattccg ttgaggagga taatgctcat gagaagtgca agaacagcgt
      361 aggtttccac catcgcggga agaatgacgg cttttcccgt ctcctcgggg cgttttgcga
      421 tcatctgaat gcaggccgcg gatgtcttcc cctgtgcaat cgccgaaaag aaaccgccga
      481 cagcaatggg aaggcaggca aagaaaatgc cgagtccctg ccatacgttc acggtaactc
      541 cagcgccgcc gccaagaaga ccaactttaa gaatggcgaa gaaggcgata agaagaccgt
      601 agatgccctg agtgccggga agagcctgga gaagaagcac gagaccaaat ttaccggggt
      661 cctccgtcat aactcctgct cctgcttctc ctgcgattcc aacaccgatt gctgaacctg
      721 caccagccca tccagccgcc attgccgctc caagtattgc caacatgatt ccaagaagat
      781 ccatagttat agacacactc ctctcgtatt cgtgaaaaat ctgaacagtt tattcttcgg
      841 gttcattttt tattgcgaca aatgctgtct tatacgtcag aggagaaaag actgttcccc
      901 ctcctgtata gaatttgcta aaaaactcga catactggag acgcagagag tgaacaaaag
      961 cgcccagtac gttgacagct atactgaaaa tgtgccctcc tatgagaaga aggagtgcga
     1021 tgatccagcc aatatagggc acgtcccccg caagaccggc gagcatgttg ataattacgc
     1081 ctaccgcaga agttgcaagc cctagtgcaa gcagacggct gtagctcaat acgtcgccaa
     1141 gatacgatgt gacgctgtac agactcagca atccggaaac cgcttttccg ataatgcctt
     1201 ctttttctct cccttgtgtt gcgatgagca ccgcagctcc aaggaacgta agcgttttcg
     1261 caaggaaaga tgtctgagcg gagataaaac cgcttgctac tcctccccaa agaagcagtc
     1321 ccgtaagaaa gacaatccaa ccgcccgtgt ccgcaaaggc ccccatatag ttccctttcc
     1381 gcagagcatc gtagaatgca ataaagagtc cgaagaaaag ctggagaatg ccaagtgcga
     1441 gtgaaattgc aaggaaaatc atgggattct ccatggggtt aaggacgagc ataaagttct
     1501 ttagcggacg caggaaggca aaaatggaaa aagcgtctac aagatctccg aaccagcttc
     1561 cggtaagcgc cccaaccacg attgtggaaa caccggaaag aacgaagagt ttgaagaacc
     1621 ccttaaaact ctcgggcatt ttcttatact ttctgagaaa ccacaagaag aatcctatca
     1681 tgatagctcc gtaaccggca tcgccaagac acataccgaa gaagatgaaa aagaacggtg
     1741 ctagcagcgg tgtggggtca atttctccgt acttaggcgc gccgtagagt ctcgtcaggt
     1801 tttcaaacgg gaaacaccat gccgagttga ctatgtgtga gggcggtgag tcttcaggtt
     1861 tcggatcaaa cagaaagagt tctacagata aaccgaaggg ggtaattgcc ttcgtgagtc
     1921 tctgaacggc agatgcggga acccagccgc gaagcatgat tacctgttct gtggaagaac
     1981 cggaagaaat tgcttggtac ctgtcacgca agacagtcca gtaatcactc aaaatacgca
     2041 ctgagggaac ccactgttcc gccgcgccgg caatctctgc aagaacgcgt tcttcttcag
     2101 atgccaaggc gacctttctg tccgagagag cgagtcgttc ctcctctggt gtttttttga
     2161 gagaaagagg tacgtccact ctgctaaatg agttgcgagc aagaagatct gtgatttgcg
     2221 ccgtcttttg ttcgtcgaaa aagacagcaa tccacccttc cgtatccttt tctccctgag
     2281 ttgaggtaaa aacctcaccc atagataaaa gaagcgcctc agtttcttct ctccaagccg
     2341 gaattttcga aacaggcatc gttcccagaa tgccgcgcac gcgttcggtt ccggaggtga
     2401 gaagttcaaa cggataaggg aagtcggaga gcgcgttcag aagcgtctcc gtcgatgcga
     2461 tctgtgtgag ttcagagcga atttccataa ggcggcgttc aagcgatctc acactcgcag
     2521 ataggctcat gagatctgtt ctgtcttcaa gagtttttcc atcgtgcaac gagattgttt
     2581 ctcgttctcc catggttcta gccaaggaag aaacattgtc cttgaaataa ggttcaagga
     2641 atcgaaggag aaaacgcgct tcgctgagga gtccctccac ccgtgataag tcagtgtgaa
     2701 caggcgcggt actttctgac actgattccg acccgaaagg gacaacttca aaggatcctg
     2761 tcttctgaag agcgtgcagg acattgtcca aatcggcttt gtgaacgtat aactcggctt
     2821 ttttaattct cgctactgcc atatcgagaa atcacctctt ccgaaatcca ttgtgccgcg
     2881 ctatttacct tctgtccgta ctgcttggca agttgctcgg acttcttttg gccggcctca
     2941 acagactgtt ttgctttttc ttccgcttct ctttccacag aggctgtatt ctctttgaaa
     3001 agcctgaatg ctttctgttt tgcctccttg gtccggcgct caacttcgtt ctgagcttcg
     3061 ttgatcattt gagcagcccc gttctttgca tccttcacaa tgtcttttga acgagcttcg
     3121 acttccttga tttcttcagc cagatttgct gccatataaa cccacctcct tccttggatt
     3181 gaacgcgagt gcacacgctg tgcacacggt caacataaaa acagtgacta ttttattacc
     3241 agacaaaatt tgtgtcaaac gtaacaatat gtccagatca taatcaacaa aacgaaacaa
     3301 ggcaagagtc ttaaagaata ttttctttca ggagaaaagt cagtatcagg ggattgggga
     3361 aaagaattgg agcggacaac gggattcgaa cccgcgaccc ttagcttggg aagctaatg
//
LOCUS       DAOTIL010000203         3407 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminobacteriaceae bacterium isolate AMR_MDS_3026
            NODE_32918_length_3407_cov_3.539081, whole genome shotgun sequence.
ACCESSION   DAOTIL010000203 DAOTIL010000000
VERSION     DAOTIL010000203.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868050
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminobacteriaceae bacterium (wastewater metagenome)
  ORGANISM  Aminobacteriaceae bacterium
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae.
REFERENCE   1  (bases 1 to 3407)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3407)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:19:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,224
            CDSs (total)                      :: 3,198
            Genes (coding)                    :: 3,184
            CDSs (with protein)               :: 3,184
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3407
                     /organism="Aminobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_32918_length_3407_cov_3.539081"
                     /isolate="AMR_MDS_3026"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:3034609"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1990
                     /locus_tag="P5201_05385"
     CDS             <1..1990
                     /locus_tag="P5201_05385"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HRV98000.1"
                     /translation="ETVLLMLVYFQDEQGNFISPSRSVALVKDACVFPLFGLWSMMPE
                     AGAFGGSVLQARRHGEKAAEIALRVLAGVPPSAIPAETDTEYVPMFDFGELKRFRLSR
                     SDLGQSSVVLNEPERISSGSWKIIIINAVVVLASIGYVALLLVNERRRKRAAKEIALR
                     KSMWEGLFRNAPDAYVVFGDDNTVLNVNEKFCEVFGYEYSEAIGRDIDSLVAKGTDAE
                     EEAAEISRKVLNGEAVYSEGVRRKKDGNTIHVGIQGVRFPVREGCARGYGIYSDISER
                     RRNEEEMNRRLLSEAAISSISARLLKESGLIGIPPSLEEMASFTGAKEGFLIEFSTPG
                     EIVRELLFSEGRAVWSASPHISEVFPESDLRTITQYLWEKGRLLLDDRLPAPLCEVDR
                     LWELKRLFGAPLLILPVYSERMVSGWLGLQLPESPLLSSHESFLGMFCDLVGGVLLQQ
                     KRRSMTSERARILDGTSRGIVEILGRTLAMKDPYTVGHQFEVARLARAMAERGGYDEA
                     FLERVYYAGLVHDLGKIVIPSSILSKPGRLNTVEFEIIKNHALHGWEILSSTEFPWPL
                     ADIVVQHHERLDGSGYPYGLKGAEIRTEARFIAVADVVEAMTSHRPYRPGLGIERALE
                     EIRTHSGTWFDPLVVELCLAVIREGFVFDDKSYKVALP"
     gene            2100..3194
                     /gene="buk"
                     /locus_tag="P5201_05390"
     CDS             2100..3194
                     /gene="buk"
                     /locus_tag="P5201_05390"
                     /EC_number="2.7.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013049217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047761 - butyrate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006113 - fermentation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="butyrate kinase"
                     /protein_id="HRV98001.1"
                     /translation="MTLHVLAINPGSTSTKIAWFTDGREIWRETVRHDPEALGVFPSV
                     AEQFQFRLDTIAEAVASKGCSFDDLSAVAGRGGLVEPIPGGTYAVDDVLLKRLRMGKP
                     WEHASNLGGILADAICRPRNIPAFIVDPVAVDEMNPAAKVTGLPELPKLSLGHALNVK
                     ATVRRAAKDLGEPWDSMNFIVAHIGGGITVCAHEKGRMTDLNNANDFGPFSPERAGGL
                     PAGDLLRFCFESGLTEKEVLRKVVGKGGLAAYTGTSDVREVKALIAEGDEAARRAYEA
                     MAYSVAREIGAMAAALSGKVDAILITGGVAYDNDFVALVQKRVQWIAPVLIYPGEDEM
                     PALAEGAFRVLQGEELQKVYSEYISGGDRS"
     gene            3191..>3407
                     /locus_tag="P5201_05395"
     CDS             3191..>3407
                     /locus_tag="P5201_05395"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate butyryltransferase"
                     /protein_id="HRV98002.1"
                     /translation="MTMQGIDFLLAESKKGRSMTLALACPYGDDALGAACRAQREGIV
                     RTILIGDEGKIRSLAERDGYDLSDISVL"
BASE COUNT          660 a          965 c         1112 g          670 t
ORIGIN      
        1 cgagacagtg ctgctcatgc tggtgtactt tcaggatgag caaggtaatt ttatctctcc
       61 ctcccgcagc gttgctctcg taaaagacgc gtgcgttttt ccgctcttcg gactctggag
      121 catgatgccc gaggctggag cattcggcgg cagcgttctg caggcccgcc gtcacggaga
      181 aaaggccgcg gagattgcgc ttcgcgtgct cgcgggcgtc cccccctcgg ctatccccgc
      241 tgaaaccgat acggagtacg tgccgatgtt cgacttcgga gagctgaagc gcttccggct
      301 ctcgcgaagt gatctggggc agagttcggt ggtgctcaac gagccggagc gtatttcttc
      361 ggggagctgg aaaatcatca tcatcaacgc tgtcgttgta ctcgcgtcca tagggtatgt
      421 cgcgctgctg ctggtcaacg aacgcaggcg gaagagagcg gcaaaagaga tcgcgctccg
      481 gaaatccatg tgggaggggc tcttcaggaa cgctccggac gcatacgttg tcttcggaga
      541 cgacaacaca gtcctgaatg taaacgagaa attctgcgag gtcttcggtt acgaatatag
      601 cgaggcgatc ggacgagata tcgacagcct ggtcgcaaag gggacggatg cggaagagga
      661 agcggcggag atctcccgga aggtcctgaa cggcgaggcg gtctattccg aaggagtgcg
      721 aagaaagaag gacggaaaca ctattcatgt gggtattcag ggggtgcgct tccccgtgcg
      781 cgagggctgc gcgcggggtt acggtattta cagcgacatc tcggagcggc gccgcaatga
      841 agaggaaatg aaccggcgtc ttctctccga ggccgcaatc tcctccattt ccgcgcgcct
      901 cctgaaagag agcggactta taggcattcc cccgtcgctt gaagagatgg cttcgtttac
      961 gggagcgaag gaaggatttc ttatcgaatt ctccacgccg ggcgagattg tccgcgagct
     1021 gctcttctcg gaagggcggg cggtatggag cgcttcgccg catatctccg aagtcttccc
     1081 tgaatccgac cttcgcacga tcacgcagta tctctgggag aaggggcggc tgctgctgga
     1141 cgaccgcctg cccgcgccgc tctgcgaggt ggatcggctt tgggagctga agcggctctt
     1201 cggagcccct cttctgatac tcccggtcta ctcggaacgg atggtgagcg gctggctcgg
     1261 gcttcagctt ccggagagtc ctcttctttc ctcccacgaa tcgttcctgg gaatgttctg
     1321 cgaccttgta ggcggagtcc tgctgcagca gaagcggcgg agcatgacgt cggagcgcgc
     1381 gcgcatcctc gacggtactt cgcgcggaat cgtcgagatc ctcgggcgga cgctcgccat
     1441 gaaggatccg tacaccgtgg ggcatcagtt cgaggtggcg cgccttgccc gggcgatggc
     1501 ggaacgcgga gggtatgacg aggcttttct ggaacgggtg tattacgccg gtcttgtcca
     1561 cgacctgggc aagatcgtga ttccgtcgtc catcctctca aaaccgggga ggctgaacac
     1621 tgtggagttc gagatcatca agaaccacgc gttgcacgga tgggaaattc tctcttcgac
     1681 ggagtttccg tggccgctcg ccgatatcgt agtccagcac cacgaacgcc tcgacggatc
     1741 ggggtacccg tacgggctca aaggggcgga gatccgcaca gaagcgcgtt tcatcgccgt
     1801 agccgacgtg gtggaggcga tgacctccca caggccgtac cgtcctggac tggggatcga
     1861 gagagccctg gaggagatcc gcacgcacag cggaacgtgg ttcgacccct tggtggtgga
     1921 gctgtgtctt gcggtgatcc gggagggctt cgttttcgac gacaaaagtt acaaagtcgc
     1981 cctcccgtga gcctttgtcc gggagaacaa tatactgtat aatgagttct gttaatagct
     2041 tttgaatctg aaaacagagg acagaagaga tgatcgcggt tccgaagggg agggtctgta
     2101 tgacgttgca tgttcttgca atcaatcccg gctccacaag cacgaaaata gcctggttca
     2161 ccgacgggcg ggagatatgg agagagacgg tccggcacga tcccgaggcg ttgggcgtct
     2221 tcccgtcagt agcggagcag tttcagtttc gcctggatac catcgccgag gcggtggcgt
     2281 cgaaagggtg ttccttcgat gacctctccg cggttgcagg gcgcggcggt cttgtcgaac
     2341 cgattcccgg cggcacgtac gccgtggacg acgtcctcct gaagcgtctc cggatgggga
     2401 agccgtggga gcacgcctcg aacctcggcg gcatcctcgc cgacgcgatc tgcaggccga
     2461 ggaatatccc ggcgtttatt gtcgatccgg tcgccgtcga cgagatgaac cccgcggcga
     2521 aggtcactgg cctccccgaa cttcccaaac tctcgctggg ccatgcgctc aacgtgaagg
     2581 ccaccgtgcg ccgtgccgcc aaggatctcg gcgagccatg ggattccatg aacttcatcg
     2641 tcgcccatat cggcggcggc attactgtct gcgcccacga aaagggtcgg atgaccgacc
     2701 tgaacaacgc caacgacttc ggcccgttct ctcccgagag agccggaggc ctgcctgccg
     2761 gggacctgct tcgattctgc ttcgagagcg gccttacgga gaaggaggtt ctccgcaaag
     2821 tcgtggggaa gggcggcctt gccgcctaca ccggcacgag cgacgtgcgc gaggtgaagg
     2881 cgctgatcgc cgaaggagac gaggccgcga ggcgcgcata cgaggccatg gcgtactcgg
     2941 ttgcccgcga gatcggcgcg atggccgccg cgctctccgg aaaggtcgac gccattctca
     3001 tcaccggcgg cgtggcgtac gacaacgatt tcgtcgcgct cgtacagaag cgcgtccagt
     3061 ggatcgcccc cgtgcttatc tatccgggcg aggacgagat gcccgcgctc gcggaagggg
     3121 cgttcagagt cctccagggc gaggagctgc agaaagtata ttccgagtac atctcaggag
     3181 gcgatcgttc atgaccatgc agggtatcga ttttcttctt gcggagagta aaaaagggcg
     3241 ctccatgacg cttgcgctcg cctgtcccta cggggacgac gcgttgggcg cggcgtgcag
     3301 ggcgcagcgt gaagggatcg tcaggacgat cctcatcggc gacgagggga agatacgctc
     3361 gctcgcggag cgagacggct acgacctctc ggatatttcg gtcctcg
//
LOCUS       DAOTIL010000204         3400 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminobacteriaceae bacterium isolate AMR_MDS_3026
            NODE_33009_length_3400_cov_2.935127, whole genome shotgun sequence.
ACCESSION   DAOTIL010000204 DAOTIL010000000
VERSION     DAOTIL010000204.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868050
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminobacteriaceae bacterium (wastewater metagenome)
  ORGANISM  Aminobacteriaceae bacterium
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae.
REFERENCE   1  (bases 1 to 3400)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3400)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:19:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,224
            CDSs (total)                      :: 3,198
            Genes (coding)                    :: 3,184
            CDSs (with protein)               :: 3,184
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3400
                     /organism="Aminobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_33009_length_3400_cov_2.935127"
                     /isolate="AMR_MDS_3026"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:3034609"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1000
                     /locus_tag="P5201_05400"
     CDS             <1..1000
                     /locus_tag="P5201_05400"
                     /inference="COORDINATES: protein motif:HMM:NF012927.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRV98003.1"
                     /translation="ARAAAREPFGPSHSPLCLLQLQDAGRYRHATGTTPGLTVRYPLL
                     DRRAKVPEHVEPLSDADIEAVQDRMVEAAVRAEKLGFDGIDIKSCHRYLASELLSAHL
                     RPGRFGGGFDGRTRFLRETTERIRSAVSNPSFLVTSRMNLFDGFPYPYGWGCDRSDPP
                     VPDMSEPLQLVGILKGLGLNLLTTSTGTPYYNPWLVRPFDRLIPGNIEAPEHPLEGVA
                     RLFALTARVRKEYPDLPTAGSGYTWLRQFGVFAAAANVARKAVTFAGFGRTGFAYPEL
                     PRDVLEKGAVDPKKVCVSCSLCSEVMGQGGSTGCYAKDREVYGPMLKAHRASRKEH"
     gene            1030..2190
                     /locus_tag="P5201_05405"
     CDS             1030..2190
                     /locus_tag="P5201_05405"
                     /inference="COORDINATES: protein motif:HMM:NF024850.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HRV98004.1"
                     /translation="MKNGTRKWLFCAVLVVLLAGLTGMAAAKELLIGNMQDISGPTSV
                     WGNAVTRGAEIAIEKINAAGGIDGAMLKLVTMDTKANVQEAIKGYNSLVSSGVVAVVG
                     PPVSNIGLALGPIANKAGVPVVGSFIDPRVTVGEDGKPHAAMFLMQPSSIQYSEIMAS
                     YTIDVLGLKKVGVLYDQSNAFAVSLMKPYVDYIKAAGGEIVSEQVYTKGDKDFKTQLL
                     KIKDAGADCLYFPNYIQDAVLAMNQRKQVGLDVPVIGGLDFAPPFASLVNDPEATNDI
                     YLANNFSETEPQLKEVWEIYKTKYGEDPVNKVFLGYDKVLLIREAMKLGGGTSAAQVM
                     AGLSQVKELQGTTGVLTISPETHQPVGLSMVMYKIEKGKYVELGRYVPEKHK"
     gene            2300..3190
                     /locus_tag="P5201_05410"
     CDS             2300..3190
                     /locus_tag="P5201_05410"
                     /inference="COORDINATES: protein motif:HMM:NF014684.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0015658 - branched-chain amino acid
                     transmembrane transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HRV98005.1"
                     /translation="MLVQNILNGISLGSVYALIAVGFALIFNVLKFSNFSQGGVMTVT
                     AYAGYVASRYLSDRLDSLALFAAVLAAAALSGAVIAAAVERLAFRRLRRTKSEPVYYF
                     ISSITMGILLENLVTIYASSNFYSYPRFFTTSAVPLFGVNVAVTDMMMFALSITSLLA
                     LLFILHRTKIGLGIRAACFDVNTVQLMGMNPDFLVTTALMISGALGGVSGVFLGMNYT
                     LYPQLGQMVVKGFVASVLGGLGSIHGAVIGAVLLGVLEIFFISWVGAGWAPVFVFIVM
                     LAFLLVRPRGIAGIIVQEKA"
     gene            3201..>3400
                     /locus_tag="P5201_05415"
     CDS             3201..>3400
                     /locus_tag="P5201_05415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008713291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HRV98006.1"
                     /translation="MDYFVSTVIFTGIALIGVLGVYLITGLTGLFSLGQAAFMAIGGY
                     VSAVLIRNYSVPVVPAVVLAIAA"
BASE COUNT          586 a         1084 c         1085 g          645 t
ORIGIN      
        1 ggcgcgcgcc gcggcccgcg aacccttcgg cccgtcgcat tctcctctct gcctgcttca
       61 gcttcaggac gcggggcgtt accggcacgc gacggggacg acgcccggcc tgacggtgcg
      121 ctatccgctg ctcgacaggc gggcgaaggt tcccgaacat gtggagcccc tttccgacgc
      181 cgacatcgag gcggtgcagg atcgcatggt cgaggccgcc gtccgcgcgg agaagctcgg
      241 cttcgacggc atcgacatca agagctgcca ccgctacctc gcctcggaac tgctttcggc
      301 ccatctacgc cccggaaggt tcggcggcgg tttcgacggc aggacgcgct tcctgcgcga
      361 gacgacggag cgcatccgct cggctgtctc gaacccttcg ttcctcgtga ccagccggat
      421 gaacctcttc gacggcttcc cgtaccccta cggctggggg tgcgaccgct ccgatccgcc
      481 cgttcccgac atgagcgagc cgctgcagct cgtcggcatc ctcaaagggc tcggactgaa
      541 ccttctgacc acctccaccg gtacgcccta ctacaatccg tggcttgtgc gcccgttcga
      601 ccggttgatt ccggggaaca tcgaggcgcc ggaacatccg ctcgaaggcg tcgcgcgcct
      661 ctttgcgctg acggcaaggg tacggaaaga gtacccggat cttccgacgg ccggttcggg
      721 gtacacgtgg cttcgacagt tcggcgtgtt cgccgccgcc gccaacgtgg cgcggaaggc
      781 tgtgacgttt gcgggctttg gacggacggg gtttgcgtac cccgagttgc cgagagacgt
      841 tctggagaag ggggcggtcg atccgaagaa ggtgtgcgtt tcgtgcagtt tgtgcagcga
      901 ggtcatggga cagggaggtt ccacagggtg ctacgcgaaa gaccgggagg tctacggccc
      961 catgctcaaa gcgcataggg cgtcccggaa agagcactaa atacatttga aagcgggagg
     1021 gaaaaaagca tgaagaacgg tacgagaaag tggttgttct gcgcggtgtt ggttgtactg
     1081 cttgccggtc tgaccggtat ggcggcggcg aaggagcttc tgatcggcaa tatgcaggac
     1141 atcagcggcc ccacgtcggt gtggggaaac gcggtgacgc ggggcgcgga gatcgccatc
     1201 gagaagatca acgcggcggg cggcatcgac ggcgcgatgc tgaagctggt cacgatggac
     1261 acgaaggcca acgtccagga ggccatcaag gggtataaca gcctcgtgag ttcgggcgtg
     1321 gtcgccgtgg tcggacctcc cgtcagcaat atcggactcg cgctcggccc catcgccaac
     1381 aaggcgggcg ttcccgtggt cggcagcttc atcgacccgc gcgtcaccgt gggcgaggac
     1441 ggcaagccgc atgccgcgat gttcctgatg cagccctcca gcatccagta ctccgagatc
     1501 atggccagct atacgatcga cgtgctcggc ctgaagaagg tcggcgtgct ctacgaccag
     1561 tcgaacgcct tcgcagtctc cctgatgaag ccgtacgtcg actacatcaa ggccgccggc
     1621 ggcgagatcg tctccgagca ggtgtacacg aagggcgaca aggacttcaa gacgcagctg
     1681 ctgaagatca aggacgcagg cgcggactgc ctgtacttcc ccaactacat tcaggacgcc
     1741 gtgctggcga tgaaccagcg caagcaggtc ggcctcgacg ttcccgtgat cggcggactg
     1801 gacttcgcgc cgccgttcgc ctcgctcgtc aacgaccccg aggcgaccaa cgacatctac
     1861 ctcgccaaca acttctccga aacggagccc cagctcaagg aagtctggga gatctacaag
     1921 acgaagtacg gcgaggatcc cgtgaacaag gtcttccttg gctacgacaa ggtcctcctc
     1981 atccgggagg ccatgaagct cggcggcggg acctccgcgg cgcaggtaat ggcggggctg
     2041 tcgcaggtga aggagcttca gggaaccaca ggcgttctca ccatctctcc ggagacgcac
     2101 cagcccgtcg gcctctccat ggtcatgtac aagatcgaaa agggcaagta cgtcgaactg
     2161 ggccgctacg tccccgaaaa gcataagtag acgacctgat actataattt agaggccgcc
     2221 ctttctcttt cgaaaagcgg ggcggcctct catttcttcc catccttccg ccgccgggag
     2281 gggcaaagga gctggtttca tgctggtgca gaacatcctc aacggtatct ccctcggatc
     2341 cgtctacgcg ctcatcgcgg tggggttcgc gctcatcttc aacgtcctca agttctcgaa
     2401 cttctcgcag ggcggcgtga tgaccgtgac cgcgtacgcg gggtacgtcg cctcccgcta
     2461 cctctcggac cggctggatt cgcttgccct gttcgcggcg gtgctcgcgg cggccgcgct
     2521 ttcgggggcg gttatcgccg cggccgtcga gcgtctggcg ttccggcggc ttcgccggac
     2581 gaagagcgag ccggtgtact acttcatctc gtcgatcacg atgggcatcc tgctggagaa
     2641 cctggtcacg atctacgcca gcagcaactt ctactcctac ccccgtttct tcaccacctc
     2701 ggccgtgccg ctgttcggcg tgaacgtcgc cgtgacggac atgatgatgt tcgccctctc
     2761 catcacctcg ctgctcgcgc tgctcttcat cctccaccgg acgaagatcg ggctgggcat
     2821 ccgcgccgcc tgtttcgacg tgaacaccgt gcagctcatg gggatgaacc ccgacttcct
     2881 ggtgaccacc gcgctgatga tctcgggcgc gctggggggc gtcagcggcg tcttcctggg
     2941 gatgaactac acgctctacc cgcagctcgg gcagatggtg gtgaaggggt tcgtggcctc
     3001 tgttctcggc gggctgggga gcatccacgg cgcggtgatc ggcgcggtgc tcctgggtgt
     3061 cctcgaaatt ttcttcatct cgtgggtcgg ggccggatgg gcgccggtct tcgtcttcat
     3121 cgtgatgctc gccttcctcc tcgtgcgccc caggggaatc gcgggaatca tcgtgcagga
     3181 aaaggcgtag ggggctcgcg atggactact tcgtctccac cgtcatcttc accggaatcg
     3241 cgctcatcgg ggtgctcggc gtctacctca tcacggggct cacggggctt ttctcgctgg
     3301 ggcaggccgc gttcatggcc atcggggggt acgtctcggc ggtgctcatc cgaaactact
     3361 ccgtacccgt cgttcccgcc gtcgtcctcg ccatcgccgc
//
LOCUS       DAOTIL010000205         3391 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminobacteriaceae bacterium isolate AMR_MDS_3026
            NODE_33124_length_3391_cov_2.942146, whole genome shotgun sequence.
ACCESSION   DAOTIL010000205 DAOTIL010000000
VERSION     DAOTIL010000205.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868050
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminobacteriaceae bacterium (wastewater metagenome)
  ORGANISM  Aminobacteriaceae bacterium
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae.
REFERENCE   1  (bases 1 to 3391)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3391)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:19:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,224
            CDSs (total)                      :: 3,198
            Genes (coding)                    :: 3,184
            CDSs (with protein)               :: 3,184
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3391
                     /organism="Aminobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_33124_length_3391_cov_2.942146"
                     /isolate="AMR_MDS_3026"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:3034609"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..409
                     /locus_tag="P5201_05420"
     CDS             <1..409
                     /locus_tag="P5201_05420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006584028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HRV98007.1"
                     /translation="TGRTLMGEETTALDIPRLCAACGVKEQHTHVVDPYNLKACRDAV
                     DVAYAAEETSVIVTTRPCALIKEVQKSRASLHCVVDAEKCVLCSSCLKLGCPAITKQE
                     GRIVIDSVACNGCSLCVQVCPKQAISREGELHE"
     gene            402..983
                     /locus_tag="P5201_05425"
     CDS             402..983
                     /locus_tag="P5201_05425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006301757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="indolepyruvate oxidoreductase subunit beta"
                     /protein_id="HRV98008.1"
                     /translation="MNKNGKNVLLVGVGGQGTILASKLLSEGLLALGYDVKMSEIHGM
                     AQRGGSVSTHVRYGARVYSPVISAGEADVLVAFEKMEALRWLSYLSPEGMLIVNNHEI
                     YPMPVILGMSEYPARLEETLAEAATRMLLIEAADEAAALGNPRVSNVVLLGAVVKSLG
                     LEDPCWAETLRSCVPPHALQVNERAFRKGLSLV"
     gene            1256..1756
                     /locus_tag="P5201_05430"
     CDS             1256..1756
                     /locus_tag="P5201_05430"
                     /inference="COORDINATES: protein motif:HMM:NF025946.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015036 - disulfide oxidoreductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HRV98009.1"
                     /translation="MIDFTKGYSFNEYLEKGYEEERDRQRRACSRAVFSPAFEEAARN
                     VNKPLKLAAFAEIYCPDSVVTMSFVHRIAELNPKIELAVFPRSPFEKELEALTGSSRI
                     PTLLFFDEKMNLAGSYVELPKALKEDMRDLDAGEKSALTLDYRRGKYNDILQAELTEI
                     LSLFAS"
     gene            1835..2368
                     /locus_tag="P5201_05435"
     CDS             1835..2368
                     /locus_tag="P5201_05435"
                     /inference="COORDINATES: protein motif:HMM:NF014471.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015109 - chromate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015703 - chromate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromate transporter"
                     /protein_id="HRV98010.1"
                     /translation="MPLSSIFLLFLRIGAFTFGGGLVMLGFIEKELRSTARLSSEEIA
                     DMMVLATAFPGPVAANMAFLAGKRLAGIAGSFCALLGTVIPPFLTILLLSKAVIHFMN
                     EPWLAAFFLGAACAVVVIIGRVVYSMTRVSCGGRWKDIAVFAAVGILLISTEVHPFFA
                     LAGGVTFRLLLGEEDAG"
     gene            2365..2898
                     /locus_tag="P5201_05440"
     CDS             2365..2898
                     /locus_tag="P5201_05440"
                     /inference="COORDINATES: protein motif:HMM:NF014471.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015109 - chromate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015703 - chromate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromate transporter"
                     /protein_id="HRV98011.1"
                     /translation="MSVLGTLALVFARIGLGAFGGGLATIPFIHFELVASRAWLSERG
                     FAEVVSLAQMTPGPLAVNAATFVGYRIAGVAGASVATFSVIAAPLLVLGLALFLISRA
                     SEKWKTRVDRLQRALRPAVSGMLFAAFWMVARSLTGDWRLWVMTAGVFMLIGQEYFKR
                     YPQLLLLIAGMAGVLFL"
     gene            2925..>3391
                     /locus_tag="P5201_05445"
     CDS             2925..>3391
                     /locus_tag="P5201_05445"
                     /inference="COORDINATES: protein motif:HMM:NF014750.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016625 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, iron-sulfur
                     protein as acceptor [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde ferredoxin oxidoreductase N-terminal
                     domain-containing protein"
                     /protein_id="HRV98012.1"
                     /translation="MEMKIARVNLTESTIEIFSAEDELLRKYLGGSGLGTYYLFKYGS
                     PKCAALSPENPLLFMNGPFQGTGIPTSGRHQVLAKSPLTDMFGESDCGGAFGYHMRKA
                     GFDGIILLGKAPSPVYIALLDGKIEIRSASSLWGKDTFETEEFLLELEGKPAGV"
BASE COUNT          711 a          843 c          987 g          850 t
ORIGIN      
        1 cacgggaagg acgctcatgg gcgaggagac gaccgctttg gacatcccgc gtctctgcgc
       61 cgcctgcggc gtcaaggagc aacataccca cgtcgtcgat ccatataacc tgaaggcgtg
      121 tcgcgatgca gtggatgtag cgtacgccgc agaagaaaca tcggtgatcg tgacgacccg
      181 tccctgcgct ttgatcaaag aggttcagaa aagcagggcc tcgctccact gcgtcgtcga
      241 tgcggagaag tgcgttctct gctcttcgtg cctcaaactc ggctgtccgg cgatcacgaa
      301 acaggaaggc cggattgtca tcgactccgt cgcctgcaac gggtgttctt tgtgcgtgca
      361 ggtctgcccg aaacaggcta tttcgaggga aggtgagctt catgaataag aacggaaaga
      421 acgtccttct tgtcggcgta ggcggtcagg gaacgattct cgcgagcaag ctcctctcgg
      481 aaggacttct tgcactcggg tatgacgtga aaatgtccga gatccacggg atggcgcaac
      541 gaggcggcag cgtctccacg catgtccgct acggcgccag ggtctactct cccgtgataa
      601 gtgcgggtga ggcggacgtc ctcgtcgctt tcgagaagat ggaggcgctt cgttggcttt
      661 cctacttgtc tcctgaaggc atgctcatcg tgaataatca tgagatctac ccgatgcctg
      721 ttatcctcgg catgtcggag tatcccgctc gacttgagga gacgcttgcc gaagccgcga
      781 ctcgaatgct tttgattgaa gcggcggatg aggcggctgc gttgggcaat ccgcgggtga
      841 gtaacgtcgt tttgctcgga gctgtggtga agagtctcgg gcttgaagat ccatgctggg
      901 cggaaactct tcgttcgtgc gtcccgcctc acgcgctcca ggtcaacgag agggctttcc
      961 gaaaagggct tagcctggta tagctacaaa acgaatacag cagagcagga gaatggggag
     1021 ctttctccct gttctccttt ttttacaagc caaaggtgcg ttcgtattcc atatatacga
     1081 ccgtgagcat cgtatgcggg caatagcatg aaaacgagtc catcgcagga tatctctttc
     1141 agtcctgcgg tatcggttca ggttttgagt cgcaactcgt ggaaataccc tttgagaaaa
     1201 acgtccgcga agggtaatat agactgcata ctgcaaagaa tgcggggtgg agcgtgtgat
     1261 tgattttaca aaggggtatt ctttcaatga gtaccttgaa aaagggtacg aagaagagcg
     1321 ggacagacag cggcgcgcat gttcgcgtgc cgtcttttct ccggcattcg aagaggcggc
     1381 tcggaatgtg aacaaacctc tcaaattggc tgcgtttgcc gaaatttact gcccggactc
     1441 ggttgtgaca atgtcttttg tccaccggat agcggagctg aacccaaaga ttgaacttgc
     1501 ggtttttccg cgatctccgt ttgaaaagga gctggaagcg ctgacaggtt cctccaggat
     1561 tcctacgctt ctctttttcg acgaaaaaat gaaccttgca ggcagctacg tagagcttcc
     1621 aaaagcgttg aaagaggata tgcgcgatct cgacgccggg gagaaaagcg ctcttactct
     1681 cgattacagg cgaggaaagt ataacgacat actccaggcc gagcttacgg agatactctc
     1741 actgtttgct tcatgacgtt ctccgcgtac gaaggatttg gagcgcgttc ggggcgtctt
     1801 ttttgagtgg ccgggtgttt tgggaggtcg atgaatgccg ctctcttcaa tttttttatt
     1861 gtttcttcga atcggcgcct ttacctttgg cggcgggttg gtcatgctgg ggtttatcga
     1921 aaaagagctg cgatcaactg cgcggttgtc gtccgaggaa atagcggaca tgatggtact
     1981 tgctacggcg tttcccggtc cggttgccgc caatatggca ttcctggcag ggaaacgcct
     2041 tgctggcatt gccggttcgt tttgcgctct attaggcacg gtcatccctc cgtttctgac
     2101 aatcctgctg ttgtcgaaag cggtgatcca tttcatgaac gaaccctggc ttgcagcttt
     2161 tttccttggc gcggcctgcg cagtcgtcgt cattatcgga agggtcgtct acagtatgac
     2221 tcgcgtctcc tgcggcggaa gatggaaaga tatagcggtg ttcgcggcgg tggggattct
     2281 gcttatcagc actgaagtac acccgttttt tgccctcgcg gggggagtga cttttcgtct
     2341 gctcctcggg gaagaggatg ccggatgagt gttcttggaa ctctcgcact ggtttttgcc
     2401 cgcataggcc tgggggcgtt cggcggagga ttggcgacca taccctttat ccattttgaa
     2461 cttgtcgcct ccagagcgtg gctttcagag agaggcttcg ctgaagttgt ttcattggcg
     2521 cagatgacgc ccggccctct cgccgtaaat gctgcaacct ttgttggcta caggatcgca
     2581 ggcgttgcgg gcgcatctgt tgcaacattc tctgtcattg cagctccgct tcttgtgctc
     2641 ggcttggcgc ttttcctgat ttccagagca tcggagaaat ggaaaacgag agtcgatcga
     2701 ttacagcgtg cacttcgccc tgctgtatcg ggaatgctct tcgccgcctt ctggatggta
     2761 gcccgctctc tgacaggaga ctggaggctc tgggtgatga cagcaggagt attcatgctc
     2821 atcggccaag agtacttcaa gcgatatcct caattactgc ttcttattgc ggggatggct
     2881 ggagtgctct tcctctgaac gaaaaaagaa agggatgatg gagaatggag atgaaaatcg
     2941 ctcgcgttaa tctcacggaa tcgacaatag aaattttctc tgccgaggac gaacttttac
     3001 gaaagtacct tggaggatcc ggacttggga cgtactacct tttcaagtac ggctcgccga
     3061 agtgcgcggc gctctcccct gaaaatcctc tgcttttcat gaacggtcct ttccaaggga
     3121 caggaattcc tacttcggga agacatcagg tgctcgcgaa atccccgctt accgatatgt
     3181 tcggagaatc cgactgcgga ggcgcttttg ggtatcatat gcgcaaggcc ggttttgatg
     3241 gcatcattct tcttggcaag gcgccctctc cggtgtacat agccctcctt gacggaaaaa
     3301 tcgagattcg cagcgcgtcc tctctctggg gaaaggacac gttcgagacg gaggagtttt
     3361 tgttggagct ggagggaaaa ccggccggtg t
//
LOCUS       DAOTIL010000206         3389 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminobacteriaceae bacterium isolate AMR_MDS_3026
            NODE_33148_length_3389_cov_4.461908, whole genome shotgun sequence.
ACCESSION   DAOTIL010000206 DAOTIL010000000
VERSION     DAOTIL010000206.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868050
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminobacteriaceae bacterium (wastewater metagenome)
  ORGANISM  Aminobacteriaceae bacterium
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae.
REFERENCE   1  (bases 1 to 3389)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3389)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:19:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,224
            CDSs (total)                      :: 3,198
            Genes (coding)                    :: 3,184
            CDSs (with protein)               :: 3,184
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3389
                     /organism="Aminobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_33148_length_3389_cov_4.461908"
                     /isolate="AMR_MDS_3026"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:3034609"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..288)
                     /locus_tag="P5201_05450"
     CDS             complement(<1..288)
                     /locus_tag="P5201_05450"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRV98013.1"
                     /translation="MNATNDGAMETPACPVCDLVSLLYTRNVMVYLYSSLAFVPRRVI
                     EGLAASGYSPQERGMYLEIEAERFSDVIEALGRSCGLTDPEKGATVVCFTER"
     gene            complement(285..665)
                     /locus_tag="P5201_05455"
     CDS             complement(285..665)
                     /locus_tag="P5201_05455"
                     /inference="COORDINATES: protein motif:HMM:NF019498.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HRV98014.1"
                     /translation="MCATVLRPDPAGEYFTEERCYILETSNSPDDPDVSIARARVAPG
                     VTTRWHRLSGTTERYYILSGTGRVEIGDLPPEDVVPGDAVIIPPLVRQRISNTGTDEL
                     VFLCVCSPRFLPEAYEDLGEERAQ"
     gene            817..996
                     /locus_tag="P5201_05460"
     CDS             817..996
                     /locus_tag="P5201_05460"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRV98015.1"
                     /translation="MEIKPLPALIFGRDFRERAINFVALVEEGTISPDDVHIFSCVET
                     ADEAWAAIKKACGIS"
     gene            complement(1057..1542)
                     /locus_tag="P5201_05465"
     CDS             complement(1057..1542)
                     /locus_tag="P5201_05465"
                     /inference="COORDINATES: protein motif:HMM:NF025054.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HRV98016.1"
                     /translation="MKNKNVLYEQLRLVENNPLNTEALARMIADPEDLHLVWPVAEYP
                     FNHDQWRAELDPANGTRSFLVYEGERMVGHGALAPCRGDGDSVMVRFLYIAPDMRNRG
                     AGRRLLSLLEARAKDAMGARRLILKVRTYNQRAMGCYGKYGFREYSRDGTLVLMEKKV
                     L"
     gene            complement(1532..>3389)
                     /locus_tag="P5201_05470"
     CDS             complement(1532..>3389)
                     /locus_tag="P5201_05470"
                     /inference="COORDINATES: protein motif:HMM:NF024583.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HRV98017.1"
                     /translation="KDSSSFWGELSAIVSPDGSCICGILRDVTERKTNELWLKEQNSS
                     LSMLAEISADLMAELDTDSLLQKVLRYATSAMNTEHGSISFVDFTTKERYVRWSQGPI
                     LSSFQGIRIPLGMGLGGEVIAAGKRKIVKDYRNFSNRLPDHDFDCIGTTVGVPLRWKD
                     EVFGYLSIYYEGEPREIEDARLEHLDQFAFLASLALHNARLYEDARLEIAERRRLEEE
                     LRKATLEAQRANEAKSDFLARMSHEIRTPLNILTGVSDLLLEREVDSELRENLSMISQ
                     AGKFLTEMVNDILDFSKIEAGMIEVERMPFSLSALLGDIAPMFVFHAERSGIAFRVSA
                     DPTLPDVFTGDSLRLKQILFNLLGNAMKFTKRGFVRLAVTGVPLGEFSYEIRFTVSDT
                     GVGMMPDVLDSIFEPYHQGELSVARRFGGSGLGLPISRKLAELMGGVLSVESALGRGS
                     DFTLSLPLEVARTPSDSKGTMEEEGTALPEKLSILVADDNTVNRTLLSKLLSLPGWTV
                     LMAADGQEALEIVRKDAPDVIILDLEMPRMDGCAAAKAIRKHEVESGKRSFILGLTGY
                     TRSESAPLCADAGMDDCLTKPARKFTLLAGIANYFSSGGVWNTLEDDEENEE"
BASE COUNT          740 a         1031 c          909 g          709 t
ORIGIN      
        1 gcgctcggtg aaacagacga ccgtggctcc cttctccggg tcggtcaagc cgcacgacct
       61 cccgagggcc tctatgacgt cagaaaagcg ctccgcctct atctccaggt acatgccgcg
      121 ctcctgcgga gaataccccg aggcggcgag cccttcgatc actcttcggg gaacgaaggc
      181 gagcgaactg tagaggtata ccatgacgtt cctcgtatag agcaaagaga cgagatcgca
      241 caccgggcat gccggagttt ccatcgcgcc gtcgttcgtc gcgttcactg cgccctctcc
      301 tctcccaggt cctcgtacgc ttccgggagg aagcgcggag agcagacgca gagaaagacc
      361 agctcatccg ttccggtgtt ggaaatgcgc tgccgcacca gcggcgggat gatcaccgca
      421 tcgcccggaa caacatcctc cggcggaaga tcgccgatct cgacacgccc cgttccgctc
      481 agaatatagt agcgctcggt cgtgccggag agcctgtgcc atctggtcgt gacccccggc
      541 gccaccctgg ccctggcgat ggagacgtcc ggatcgtccg gtgagttgga cgtttcgagg
      601 atataacagc gctcttccgt aaagtattca ccggccgggt cgggccgcag gacagttgca
      661 cacacgggag attccccctc tccaggataa gacgcccaag aaaaaggagc gtatcgtgcg
      721 gataatagta catcaaaagt accgtcggga acagcgactg acatcactgc tataatcggg
      781 acatcctgcg gcgcacgtga atcggaagcc cggaagatgg agatcaagcc gcttccagcg
      841 ctcatcttcg gaagggactt ccgggagcgc gcgatcaact tcgtcgcgct cgttgaagag
      901 gggacgattt ctcccgacga cgtacacatc ttctcctgcg tcgaaacggc ggacgaggcc
      961 tgggcggcga tcaaaaaggc atgcggtatt tcctgagatt tttcgaaaac aggattattt
     1021 cagcgacgtc tcggttctca cgatgacgaa ggccggttac agaacttttt tttccatcag
     1081 taccagcgtg ccgtcgcggg agtattcgcg gaagccgtac ttcccgtagc agcccatcgc
     1141 gcgctgattg tatgttctga ctttcaggat aaggcgccgc gctcccatag cgtccttcgc
     1201 gcgcgcttcc agaagagaga gcaagcgcct tcccgcccct ctgttccgca tatcgggggc
     1261 gatatacaga aaacgcacca tcacggaatc tccgtcgccc cggcaagggg cgagcgcgcc
     1321 gtgtccgacc attcgttccc cctcgtacac gaggaatgag cgcgtcccgt ttgcagggtc
     1381 caactcggcc cgccactggt cgtggttgaa aggatattcg gctaccggcc aaacaagatg
     1441 aagatcttcc ggatccgcaa tcatccgggc aagagcttcc gtattcagcg ggttgttttc
     1501 tacaagtcgc agctgttcgt acaagacgtt cttattcttc attctcttca tcatcctcaa
     1561 gcgtattcca caccccgccg gaagaaaagt agttcgctat gcccgccagc agtgtaaatt
     1621 tcctcgcagg ttttgtaagg cagtcgtcca tacccgcatc cgcgcagaga ggggcgcttt
     1681 cggaacgggt ataccccgtc aatcccagga taaacgaacg ctttcccgac tcgacttcgt
     1741 gcttccgtat ggctttcgcc gcagcgcagc cgtccattcg cggcatctca aggtccagga
     1801 taatgacgtc gggggcgtct tttcgtacaa tctccagggc ttcctgtccg tccgccgcca
     1861 ttaagacggt ccaccccgga agcgaaagca gctttgagag aagcgtcctg tttaccgtgt
     1921 tatcgtcggc cacaagaatc gataacttct ccggaagcgc ggttccttcc tcttccattg
     1981 ttcccttgga atcagatggc gttcgggcaa cctccagggg aagcgaaagc gtaaagtcgg
     2041 aacctctccc gagagcgctc tccaccgaaa ggactcctcc cataagttct gccagctttc
     2101 tgctgatcgg cagccctaag ccagaaccgc cgaaccgacg ggcaacggag agctcgcctt
     2161 ggtgatacgg ctcgaagatc gaatcaagaa catccggcat catacccacg cctgtgtccg
     2221 agacggtgaa ccggatctcg taactgaatt cgccgagagg aactccggtc acggcaagac
     2281 gtacgaaacc tctttttgta aatttcatcg cattccccaa gagattaaag agaatctgct
     2341 tcaggcgcag cgaatcccct gtgaagacgt ccggcaacgt cggatcggcg gacacgcgaa
     2401 aggcgatacc gctacgctcc gcatgaaaga cgaacatcgg cgcaatatcc ccgagaagcg
     2461 cgctcaggga gaaaggcatc cgctcaacct cgatcatgcc cgcttctatt tttgagaagt
     2521 ccagaatgtc gttcaccatc tccgtgagaa atttgccggc ctgggagatc atcgagagat
     2581 tttcgcgcag ttcggaatcg acctctcttt caagcagcag atcgctcact ccggtgagga
     2641 tgttcagcgg cgttcgtatt tcatggctca tccgggccag aaaatcgctc ttcgcctcgt
     2701 ttgcgcgctg cgcctcaagc gtcgccttcc gaagttcctc ctccaggcgg cggcgttccg
     2761 caatctccaa cctcgcatct tcgtagagtc ttgcattatg caacgccaac gaagccagga
     2821 aagcgaactg atcgagatgc tccagccgcg catcctcgat ttcccgcggc tctccttcgt
     2881 agtaaatgga aaggtagccg aaaacctcgt ccttccagcg cagcggtacc ccgactgtcg
     2941 ttccgataca gtcgaagtca tgatcaggca gcctgttgct aaagttccgg taatctttga
     3001 ctattttgcg tttgccggcc gcgatcacct cgccgccgag ccccatgccc aaaggtatcc
     3061 ggattccttg gaacgaggag aggatcggcc cctgcgacca acggacatag cgctcttttg
     3121 tggtgaaatc aacgaacgat atggagccgt gctcggtgtt catcgccgat gtagcgtatc
     3181 gcaacacttt ctggagaagg gaatccgtat ccagctcggc cataagatcc gcgctgatct
     3241 cggcaagcat cgagagcgaa ctgttctgct ccttcagcca gagttcgttt gttttgcgct
     3301 ccgtcacatc gcgaagaatc ccgcagatac agctgccgtc gggtgaaacg atcgccgaaa
     3361 gctcgcccca gaaggacgaa gaatctttt
//
LOCUS       DAOTIL010000207         3388 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminobacteriaceae bacterium isolate AMR_MDS_3026
            NODE_33163_length_3388_cov_4.048005, whole genome shotgun sequence.
ACCESSION   DAOTIL010000207 DAOTIL010000000
VERSION     DAOTIL010000207.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868050
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminobacteriaceae bacterium (wastewater metagenome)
  ORGANISM  Aminobacteriaceae bacterium
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae.
REFERENCE   1  (bases 1 to 3388)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3388)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:19:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,224
            CDSs (total)                      :: 3,198
            Genes (coding)                    :: 3,184
            CDSs (with protein)               :: 3,184
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3388
                     /organism="Aminobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_33163_length_3388_cov_4.048005"
                     /isolate="AMR_MDS_3026"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:3034609"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1289
                     /locus_tag="P5201_05475"
     CDS             <1..1289
                     /locus_tag="P5201_05475"
                     /inference="COORDINATES: protein motif:HMM:NF024240.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HRV98018.1"
                     /translation="WGNDFPGAEQWEGKGGLPVAYLEDPAFRRDVNAQALLKTLNFIY
                     YVQHELREPWSVADDEFYSEIPVTKTLGIVPDEYAEIVRRFPPMPYVRESRRLVGIET
                     PTSKTVRHNSESYRDEREGREIATSMAIGGYILDLHAGDTDADLETEFGESFASIRTD
                     MPQGPFQVPFGSFIPTEVDGLLVTEKNLSMSRLVSGALRLQPISMLTGQAAGTIAALS
                     VREGKAARALDPRLVQLHLLEAGSALSLCEYHDVPRNHLFWPGVQMSNLYGWFTPLDL
                     PSSPSAKIDDIYNNRVVLARLFGLDKGVFGVDAPLTGAEAETLLHKAFGEEKDASLYA
                     LPTFGLSSFISRGDFADALARILGYRATPEEFVSRFSDISSTSRLAGPMHFLSEKGVL
                     DRVGASGVFMPDSLLTRGAAADMTMRAVVAPRERRR"
     gene            1286..1873
                     /locus_tag="P5201_05480"
     CDS             1286..1873
                     /locus_tag="P5201_05480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013047552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1847 domain-containing protein"
                     /protein_id="HRV98019.1"
                     /translation="MKCHLCETKSCSSGEACADFESLELYQGNEEDLQLLKTASDVEA
                     LYYCTLNRLEEIMEFARRMGYTRLGIAFCVGFKEEAALLGKILSEEFEVYSACCKVSS
                     MKKADVDATLRPWVGEVSCNPVEQARRLNEAGSQFNIVLGLCVGHDSLFYRHSKAPVT
                     TFVVKDRVLGHNPVAVLYSQYLRKNLKKRPEERLK"
     gene            2049..>3388
                     /locus_tag="P5201_05485"
     CDS             2049..>3388
                     /locus_tag="P5201_05485"
                     /inference="COORDINATES: protein motif:HMM:NF014763.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cache domain-containing protein"
                     /protein_id="HRV98020.1"
                     /translation="MRKRAAALSMQTALALAFTLFVLLSAGIAGYLSQRSGRESVEEI
                     AARLRGDMATHIGEHVRHFLHIPHIINRLNADALGGGMLDVLDERSLEFHFRKQIDVF
                     PSVSSIYFGNTSGGLVNSGREAPFDSRYVIGTIGSKAGVFRKYSIDKDGNRTGLLAEV
                     PDFDARTRGWYSRAQKSGGEGVWSDVYVLFTGQDMAITASRPVYDAHGGFLGVAAVDL
                     SLSQLTAFLQALPLGEGTAFIMERSGLMLASSRGEPPFVFYEGGEARRLHAADSVHAE
                     ISGAALFMASELEKNGLLTDGEIAFESGGERCYLRTESLHGPGDLDWIIAVTAPEREF
                     MDRIDKNNRTTTLVILFAVLLSFGAALLLARRMAQPVLRLNAAARAITRGENPPELPV
                     DCEILEVGELSASFNRMAEALRETIRGLNEEIGVRKETESRVRAAMERAEDLRK"
BASE COUNT          714 a          920 c         1031 g          723 t
ORIGIN      
        1 actgggggaa cgacttcccc ggcgctgaac agtgggaagg aaagggcggc ctgcccgtgg
       61 cgtacctcga agatccggca tttcgccggg atgtgaacgc acaagcgctg ttgaagacgt
      121 tgaacttcat ttactacgta cagcacgaac tgcgcgagcc atggtccgtg gcggacgacg
      181 agttctactc cgaaattccg gtaacgaaaa ctcttggcat cgtcccggac gaatacgccg
      241 agatagtcag gcgctttccc ccgatgccgt atgtgcgaga gagccgccgt cttgtcggca
      301 ttgaaacccc aacctcgaaa acggtccggc acaactccga gagttatcgc gacgaacgcg
      361 aaggacgaga gatagcgacg tccatggcaa tcggcgggta catccttgac ctgcatgcag
      421 gggataccga cgcggatctc gaaacggaat tcggcgagag ctttgcgtcg atccggaccg
      481 atatgccgca ggggcctttt caggtcccct tcggcagttt tatcccgact gaagtggacg
      541 gcttgctcgt tacggaaaag aacctctcaa tgtcccgtct tgtgagcggc gcgctgcgcc
      601 ttcaaccaat ttcgatgctc actggacagg ctgcgggaac gattgcggcg ctctcagtca
      661 gagagggaaa agcggcgagg gctctcgacc cgaggcttgt gcagctgcat ctgctggagg
      721 cagggagcgc gctctccctc tgcgaatacc acgacgtccc gcgaaaccac ctcttttggc
      781 cgggagttca gatgtccaac ctctacggat ggttcacgcc ccttgacctg ccttcgtccc
      841 cttcggcgaa aatagacgat atctacaaca accgggttgt gctggcccgt ctcttcggac
      901 ttgataaagg ggtgttcggc gtcgacgccc cattgacggg ggcggaagcg gagacgctgc
      961 tccataaagc gttcggtgaa gagaaggacg cttcgctgta tgcccttcct acattcggac
     1021 tttcgtcttt tatctcgagg ggggattttg ccgacgcgct cgcgaggata ttagggtaca
     1081 gagcaactcc tgaagagttt gtctcccgct tcagcgatat ttcctccacc tcgcgtctcg
     1141 cgggcccgat gcacttcctc tcggagaagg gcgttctgga ccgcgtcggg gcatccggcg
     1201 tctttatgcc ggactctttg ctgacgcgcg gagctgccgc agacatgacg atgagagcag
     1261 ttgtcgcgcc gagggaacga cgaagatgaa gtgtcacctc tgcgagacga agagctgctc
     1321 ctcgggtgag gcgtgcgctg attttgaaag cctggaactc taccagggaa atgaagaaga
     1381 ccttcaactt ctcaagacgg cctccgatgt tgaagcgctc tactactgta ccctcaaccg
     1441 gctggaggaa ataatggagt ttgcccggcg gatggggtat acgcgtctgg gcattgcctt
     1501 ctgtgtcggt tttaaagagg aggcggctct tctggggaag atcctttcgg aggaattcga
     1561 agtgtactcc gcatgctgca aggtctcctc aatgaagaag gcggacgttg acgcaacgct
     1621 tcgtccctgg gtcggcgaag tgtcgtgcaa ccccgtcgaa caggcgcgtc gcctcaacga
     1681 agcgggaagt cagttcaaca tcgtcctcgg cctctgcgtg ggacacgaca gcctctttta
     1741 ccgtcactcg aaggcgcctg tcacgacctt cgtcgtcaag gaccgcgtgc tcggacacaa
     1801 cccggtagcg gttctttaca gtcagtacct caggaaaaat ttgaagaaac gtcccgagga
     1861 gaggttgaag tagagcggaa caaagggagg ccgaggagaa tgcggcctcc ctttttcttc
     1921 ggtacctcat tttttaggtg tgaccattca ggaaaatggc gcgtgcggat ggccgtgagg
     1981 ggcgtcttgt tttataatga ttcaggacgt atgacgtcgt acgtaagggt tgatagtgat
     2041 tgaagagaat gagaaaaaga gcggctgcgc tctcgatgca gacagctctc gcgcttgctt
     2101 tcacgctttt tgttctgctt tcagcgggta ttgccgggta tctgtcgcag agaagcggac
     2161 gggaatcggt agaggaaata gccgccaggc tgcgaggcga tatggcgacg catatcggcg
     2221 agcacgtgcg tcattttctc cacatacctc atattatcaa ccgcctgaac gcggatgctt
     2281 tgggcggggg aatgctggat gttctcgacg agcgctcgct ggaattccat ttcaggaagc
     2341 agatagatgt tttcccttca gtttcgagca tctacttcgg caatacgtcg ggaggactcg
     2401 tcaacagcgg cagagaagct ccttttgatt cccgctacgt catcggcacg atcggtagca
     2461 aagcgggcgt cttccggaag tactccatcg acaaagacgg caaccgcacc gggcttttag
     2521 ccgaagtccc cgattttgac gcgcggacga gaggatggta ttcccgtgct caaaagagcg
     2581 gaggcgaggg agtctggagc gacgtgtatg ttctctttac aggtcaggat atggccatta
     2641 ccgccagtcg cccggtgtac gacgctcatg gaggttttct cggcgtagcg gccgtggacc
     2701 tctccctttc gcagctgacg gcatttttgc aggcgctgcc gctcggcgag ggaaccgcgt
     2761 tcatcatgga gcgctcggga ctgatgcttg cctcttcgag aggggaacct ccatttgtct
     2821 tctacgaagg aggagaagcc cggcgcctgc atgccgccga cagcgtgcat gccgaaatca
     2881 gtggagccgc tcttttcatg gcgagcgaac tcgaaaagaa cggtttgctc acagacgggg
     2941 agatcgcttt tgaaagcggc ggggaacgct gctatcttcg cacagaatcc ctgcacggcc
     3001 cgggagatct tgactggatc atcgccgtta cggctccgga gagagagttc atggaccgga
     3061 ttgataaaaa caaccgcacg acgacgctgg ttatcctctt tgccgtgctc ctctctttcg
     3121 gcgcggcgct gcttcttgcc cgaagaatgg cgcagccggt ccttcgcctg aatgccgccg
     3181 cgcgtgcgat caccagaggg gagaatccgc ccgagcttcc tgtcgactgc gaaattctcg
     3241 aggtgggcga gctttccgcg tcgttcaaca ggatggcgga ggcgctgcgt gaaaccatcc
     3301 gcggcttgaa cgaagagatc ggcgtgcgaa aagagacgga atcccgtgtg cgcgccgcga
     3361 tggaaagggc ggaagacctc agaaaaga
//
LOCUS       DAOTIL010000208         3386 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminobacteriaceae bacterium isolate AMR_MDS_3026
            NODE_33181_length_3386_cov_4.161513, whole genome shotgun sequence.
ACCESSION   DAOTIL010000208 DAOTIL010000000
VERSION     DAOTIL010000208.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868050
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminobacteriaceae bacterium (wastewater metagenome)
  ORGANISM  Aminobacteriaceae bacterium
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae.
REFERENCE   1  (bases 1 to 3386)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3386)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:19:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,224
            CDSs (total)                      :: 3,198
            Genes (coding)                    :: 3,184
            CDSs (with protein)               :: 3,184
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3386
                     /organism="Aminobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_33181_length_3386_cov_4.161513"
                     /isolate="AMR_MDS_3026"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:3034609"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..573
                     /locus_tag="P5201_05490"
     CDS             <1..573
                     /locus_tag="P5201_05490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005658940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CheB methylesterase domain-containing protein"
                     /protein_id="HRV98021.1"
                     /translation="PEMVAIASSTGGPRALQQVLSQIPGDFPVPIVIVQHMPKDFTLS
                     FAKRLDSLSELDVVEGAEGTELRPGLAVVAPGGYHMVVRKKSPGKFACALSDAPPLLS
                     VKPSANIMFLSVADEIGGNTVGVILTGMGRDGADGAAALKMKGARIIAESQETCVVYG
                     MPKAAVEQGVVDELLPLYSIPEAMIRSVRE"
     gene            613..2673
                     /locus_tag="P5201_05495"
     CDS             613..2673
                     /locus_tag="P5201_05495"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012870052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheA"
                     /protein_id="HRV98022.1"
                     /translation="MTNMDMSQYMGAFLDEAGDNLKHLDDLTLVIEKDPGNSEAIAEI
                     FRSAHTLKGMSATMGFDRMASLTHAMEDMLDAVRKGEYSLSAGDIDLLFRSLDTLQSM
                     VDAIRSVGSDASVDTDALVKSIRAAGVNRGAEPPAAEEKALNDELSGQEQKWVTEAAA
                     MGFGVFEASVTLSSDCLLKAARAYMVVSRLDEMGDIIKTVPPVEALENEEFAHDFLVY
                     VSTHHKAEALKDAVNRVSEVASVEIRELSASPAEAASEEAVTVAAQGPTPQEVSASSP
                     VHEEKTASPKMPAATPPQRKTEAAKKQSQTVRVDIGRLDKLMNLVGELVIGRARIERL
                     VQEAHLREFDDPLSQLGRISGDIQELVTKLRMVPVSFIFERFPRLVRDLSKTLGKEIE
                     LVMEGQETELDRTVIDEIGEPMVHIIRNSIDHGIEKTEDRKKAGKPSKGIIKISAYQE
                     GSGVIIEVTDDGKGIDAAKVRAKALANGVITEEESAAMSDEEVVHLIFLPGFSMAEKI
                     TDVSGRGVGMDAVKTKVESLGGQFDISTELGVGTRVFVRLPLTLAIVLALLIKVGEET
                     YALPLENVEETILVKKENVKTVHGTPATLLRGEVLTLCDLSKMLHTPRASGEESDEYP
                     VVVVKTGKTRVGFIVDELIGQQDIVIKSLGRFLSKIKGIAGATILGDGNVALILDVAG
                     LHGR"
     gene            2702..3328
                     /locus_tag="P5201_05500"
     CDS             2702..3328
                     /locus_tag="P5201_05500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005658938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheC"
                     /protein_id="HRV98023.1"
                     /translation="MDMENFSNMQLDAMREVGNIGAGNAATALSVMLSRLVDMDVPKA
                     ELVSIYELAEYYGDPLKPVSAVFVRSEGEFTCSLIFFQDEEDAQSLVDLLISQQMAGM
                     ALDDIPPEMRDSALTEVGNIILSSFLNAINVLMGGTHSISVPGVAHDMMGSILQVIAS
                     IFGQTGEYALIVDTTLKVGDIEKNVSGKVVMLPDPGSLEILFRKLQVL"
BASE COUNT          746 a          917 c         1037 g          686 t
ORIGIN      
        1 cccgagatgg tcgccatagc ctcatcaact ggcggtccca gagcgttgca gcaggtgctt
       61 tctcagatac ccggggattt cccggttccc attgtcattg tgcagcacat gccgaaggat
      121 tttaccctct ctttcgcaaa acgactggat tctctgtctg aactcgatgt cgtcgagggg
      181 gccgaaggaa cggaattacg tccggggcta gccgtagtgg ctcctggagg gtatcatatg
      241 gtagtgcgga agaaatcgcc gggaaaattc gcttgcgctc tttccgatgc gccccctctt
      301 ctttccgtca agccgtcggc gaacatcatg tttttgagcg ttgccgacga gatcggaggg
      361 aatacggtcg gggtgattct caccggtatg ggacgggacg gggccgacgg cgcagccgcg
      421 ctcaagatga aaggggcgcg catcatcgca gagtcccagg agacgtgcgt tgtgtacgga
      481 atgccgaagg ctgcggtgga acagggcgtt gtggacgagc ttcttccttt gtactcaata
      541 cccgaggcaa tgatccgaag cgtgagagaa taacgacatg aaaagacttt acaaggctgg
      601 agatgatctg ccatgacgaa tatggatatg agccagtata tgggagcttt cctcgacgaa
      661 gcgggggaca atctgaagca cctcgacgat cttacgctcg tgatcgaaaa agacccgggg
      721 aattcggagg ctatcgccga gatcttccgg tcggcgcaca ccctcaaagg gatgtccgcc
      781 actatggggt tcgacaggat ggcttccctc acccacgcca tggaggacat gctcgacgcc
      841 gtccgaaaag gggaatattc attaagcgcg ggggatatcg acctgctctt ccgttcgctt
      901 gatacgctgc agtcaatggt cgacgccata cggtcggttg gaagcgacgc ttcggtggat
      961 accgacgctc tcgtaaagtc tatacgagcc gcgggggtta acagaggagc tgaaccgcct
     1021 gcggcggaag aaaaagctct gaacgacgag ctctccggcc aggagcagaa gtgggtgacc
     1081 gaggccgcgg ctatggggtt cggagtgttc gaagcctccg tgacgctctc ctcggactgc
     1141 cttctgaagg ctgcccgggc atacatggtt gtcagcaggc ttgacgagat gggggatatc
     1201 atcaagacgg ttcccccggt ggaagccctc gaaaatgagg agtttgccca tgatttcctc
     1261 gtatacgttt ctactcatca caaggcggag gcattgaagg acgccgtaaa cagggtgagc
     1321 gaggtcgcct cggttgaaat acgcgagctg tccgcctcgc cggcggaagc ggcttctgaa
     1381 gaggccgtca ctgtggcggc tcagggaccg acgccacagg aggtttcagc ctcgtcgcct
     1441 gtccatgagg aaaaaacggc ctccccgaaa atgcctgcgg caacgccgcc ccagcgaaaa
     1501 acggaggcgg cgaaaaaaca gagccagacg gttcgcgtcg atatcggccg tctcgataag
     1561 ctcatgaatc ttgtcggcga gcttgtcatc gggcgcgccc gcatcgagcg cctcgtgcag
     1621 gaggcgcatc tccgcgaatt cgacgatccg ctctctcagc tcggccggat ttccggcgat
     1681 atccaggagc tcgtgacgaa actgcgcatg gtccccgtct cctttatttt cgagcgtttc
     1741 ccccgccttg tccgcgatct ttcaaagacg ctcggcaagg agattgaact ggtgatggaa
     1801 gggcaggaga cggagctcga ccggacggtg atcgacgaga tcggcgagcc gatggtgcat
     1861 atcatcagaa actccatcga ccacggtatc gagaagacag aggaccgtaa aaaagctggc
     1921 aagccctcca agggtatcat caaaatatcc gcataccagg aggggagcgg cgttatcatc
     1981 gaggtcaccg acgacggcaa gggaatcgac gctgccaagg tccgtgcgaa ggcgcttgcg
     2041 aacggcgtca tcaccgaaga agagtctgcc gccatgagcg acgaggaggt tgtccacctt
     2101 atcttccttc ccggtttcag catggcggag aagatcacgg atgtctccgg caggggcgtg
     2161 ggaatggacg cggtcaagac gaaggttgaa tcgctcggcg ggcagttcga tatttcgacc
     2221 gagctgggcg tcggtacgcg cgttttcgtc agactgccat tgacgctcgc gatcgtcctc
     2281 gcgcttttga tcaaggttgg tgaagagacg tacgcgctgc ccctggagaa cgtcgaggag
     2341 accatcctcg tgaagaagga aaacgtcaag acggtccacg gtactcctgc cacgctgctt
     2401 cggggagaag tgctcaccct ctgcgatctt tcgaaaatgc tccatacgcc gcgagcctcc
     2461 ggcgaggaga gcgacgagta ccccgtggtc gtcgtcaaaa cggggaagac gcgagtcggc
     2521 tttattgtcg acgagttgat cggtcagcag gatatcgtca tcaagtcgct cggccggttc
     2581 ctctcgaaga tcaaggggat tgcgggcgcc accattttgg gcgacggcaa cgtggcgctc
     2641 attctggacg tggccggtct gcacggcaga taaatacaga aggaaggacg cggcatacct
     2701 catggatatg gaaaatttca gcaacatgca gctggatgcc atgcgcgaag tcggcaacat
     2761 cggtgcggga aacgccgcga ccgcactctc cgtcatgctc tcccgcttag tggatatgga
     2821 tgttccaaaa gcggaactgg tctcgatcta cgaactggcg gagtattacg gggatcccct
     2881 caagccggtt tccgccgttt ttgtccgctc ggaaggggag tttacgtgca gtcttatttt
     2941 ctttcaggac gaggaggacg cgcaaagcct cgtggatctc ttaataagcc agcagatggc
     3001 cggtatggcg cttgacgata ttccgccgga gatgcgggac agcgcgctca ccgaggtcgg
     3061 gaatatcatc ctgagctctt tcctgaacgc catcaacgtc ctcatggggg gaacgcacag
     3121 catctccgtt cccggagttg cgcacgacat gatggggtcc atactccagg tgatcgcctc
     3181 catcttcggg cagacgggcg agtacgccct tatcgtcgat acgacgctca aggtcggcga
     3241 tatcgaaaaa aatgtctccg gaaaggttgt catgcttcct gacccagggt cgcttgaaat
     3301 cctcttcaga aagctgcagg tgctctgagt catggaaaaa gcccatcatg tgggaatggc
     3361 ggatctcatc gttgtctcct ccccag
//
LOCUS       DAOTIL010000209         3386 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminobacteriaceae bacterium isolate AMR_MDS_3026
            NODE_33184_length_3386_cov_3.807265, whole genome shotgun sequence.
ACCESSION   DAOTIL010000209 DAOTIL010000000
VERSION     DAOTIL010000209.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868050
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminobacteriaceae bacterium (wastewater metagenome)
  ORGANISM  Aminobacteriaceae bacterium
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae.
REFERENCE   1  (bases 1 to 3386)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3386)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:19:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,224
            CDSs (total)                      :: 3,198
            Genes (coding)                    :: 3,184
            CDSs (with protein)               :: 3,184
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3386
                     /organism="Aminobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_33184_length_3386_cov_3.807265"
                     /isolate="AMR_MDS_3026"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:3034609"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1066
                     /locus_tag="P5201_05505"
     CDS             <1..1066
                     /locus_tag="P5201_05505"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRV98024.1"
                     /translation="TISKTMLSSAQFRVFVVAAGYSAATVAQSALYTDLKLFTLEEIF
                     PSIPSYVFSDNGPVIDLGGDWWGWADTYDPFVVSLPEPSGMLYFTDPGCSIRSTRCRA
                     FDRLEQTTFSVADSHLREFDGQASPKHEYHAVGRVTMKGGVPVLLPKVDPACTFLRLT
                     VPELLPGSFKIEEDGTNNLFLTLTEEYTGPLEYTVSTDRPISQLAFATIPALPLPKPH
                     DHFGIAELSKNYATIQELVAAMQEYFSTYNKNCASCFAELESEPDSDAKIAYMISHRV
                     GSCRHRAFLGFRILEPLGLPLRYLVSRTHAWLEIQVAGQWYYIDLGGCPVTILNQPTS
                     PQPGTLEPPCRGERIDAADA"
     gene            complement(952..2907)
                     /locus_tag="P5201_05510"
     CDS             complement(952..2907)
                     /locus_tag="P5201_05510"
                     /inference="COORDINATES: protein motif:HMM:NF014758.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HRV98025.1"
                     /translation="MTDFSVVGKPVLKVDSLSLACGSARYTADYEMRDPLYLAFAYSP
                     VAHGDIVSLDDSEARAMEGVTDVFHAFNTETELFTTAGQGYPEPSPYDTRLFNKRVRY
                     AGEIVAAVLAGTLSEARAAAAAVKVEYAPLPTLFDPEKAMDKDSPSLHGEDGAYAPLP
                     VPFRAEENVAGEVLFSFGDMEKGFADADFIVDETYRTHYTAHCALEPHAASAFFDEKG
                     RLVIVASTQVPFHARRTVSRVLGIPAHMIRVIKPRIGGGFGSKQEIILELYAALAAWK
                     YKRNVKAEMSRSEVFTAARTRHPMRVRIKTGVKNDGSITALDMSDVMNTGAYGAHALT
                     VLSNAGSKVLPMFNKIENMNFVGRAVYTNLPIGGAYRGYGATQGYFAFNQHMDGIARR
                     LGVDFLDFVKKHHIREGETSEVFKAIGEGTEGVSQIIRSCKLSECIDKGAEAIGWSNL
                     RGKKIANGPVVKGIGAAVSMQGSGIPQVDMASASMKMNDDGSFNLYVGATDIGTGSDT
                     ILSQIAAETLNVPLEMILILSSDTDLTPFDTGAYASSTTYVSGQAVLRCAEKVRSQIL
                     SVAEKMTGAAPESLVFKESRVVNTATGEETSFSDIACFAMYSHDQFQIQASAASMRSP
                     RQGGSSVPGCGEVGWLRIVTGQPPRSI"
     gene            complement(2900..3367)
                     /locus_tag="P5201_05515"
     CDS             complement(2900..3367)
                     /locus_tag="P5201_05515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009200606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(2Fe-2S)-binding protein"
                     /protein_id="HRV98026.1"
                     /translation="MKGSFLVNGRTNEWTFEPDATLLDVLRANGHPEVHRGCDEGVCG
                     SCSVLLEGKLVNSCQVFAASAMEKDILTVAGIGTIHEPHPIQTAFVESGAVQCGFCTP
                     AAILATLALLRKNPNPTDEEIHSALDGNYCRCTGYVKIIEAVRLASRRMSSHD"
BASE COUNT          753 a          962 c          882 g          789 t
ORIGIN      
        1 cactataagt aaaacgatgt taagttctgc gcagttccgt gtcttcgttg ttgcagccgg
       61 ttattccgct gcaaccgttg cgcagtctgc gctttatacc gacttgaagc tttttacctt
      121 ggaagaaatt ttccccagca ttccatccta tgtattcagc gataatggtc cagtaataga
      181 tctaggaggg gattggtggg gttgggcaga tacgtatgat ccttttgttg tgagcctgcc
      241 ggaaccaagc gggatgctat attttaccga tcccggctgc agtatccgca gtaccaggtg
      301 ccgagccttt gaccggctcg agcagacgac tttttcagtt gccgattctc atcttcgaga
      361 gtttgatggg caagccagcc caaaacatga atatcatgct gtgggccggg ttaccatgaa
      421 aggcggagtc cctgtattat taccaaaggt ggatccggcc tgtactttct tgaggctgac
      481 tgttccggaa ttgttgccgg gttcttttaa aatagaagaa gatggcacaa acaacctttt
      541 tctgacactg actgaagagt atacaggccc gttagaatat accgtctcga ctgaccggcc
      601 tataagccag ctggcatttg cgactatacc tgctttgccg ctgccaaagc cccatgatca
      661 cttcggtata gccgagcttt ctaaaaacta cgccaccata caagagcttg tcgctgcaat
      721 gcaggagtat ttttctacat acaacaaaaa ctgtgcttca tgtttcgcag aacttgaaag
      781 cgagccggac agcgatgcaa agatcgcata catgatttcg catagagttg gctcttgccg
      841 ccatagggca tttctcggat tccgtatcct ggaaccatta ggtctgcctt tacgttatct
      901 ggtaagccga actcatgcct ggctggaaat tcaggtagcc ggacagtggt actatattga
      961 tcttggcggt tgtccggtaa ctattctcaa ccagccgact tcgccacagc cgggcacgct
     1021 ggaacctcct tgccgtggtg agcgcattga cgccgctgat gcctgaatct ggaactggtc
     1081 atgactgtac atggcaaagc aggcaatatc ggagaaggac gtctcttcgc ctgttgcagt
     1141 attgaccacg cggctctcct tgaatacaag actctcggga gcagcgcccg tcatcttttc
     1201 cgcaacggag agaatctgcg aacgcacctt ctctgcgcat cggaggaccg cctgcccgga
     1261 gacgtacgtg gtggacgaag cgtaggctcc ggtatcgaac ggcgtcagat ccgtatcgga
     1321 ggagagaatc aggatcattt caagcggcac gttcaaagtt tccgccgcga tctgggagag
     1381 aatcgtatcc gaacccgtgc cgatatcggt ggcgccgacg tagaggttga acgaaccgtc
     1441 gtcgttcatc ttcatcgagg cgcttgccat atcaacctga ggaatgcccg agccctgcat
     1501 ggatactgcc gcgccgattc ccttcaccac gggaccgttt gctatctttt tgccgcgaag
     1561 attgctccag ccgatcgctt ccgctccctt gtcgatgcac tcgctcaact tacacgaccg
     1621 gattatctgg cttacacctt ctgtgccttc gccgatggcc ttgaagactt cggatgtttc
     1681 gccttcccgg atgtggtgct tcttaacgaa atcgagaaaa tcaacgccga gcctccgggc
     1741 gattccatcc atgtgctggt tgaaggcgaa gtagccctgg gttgccccgt agccgcgata
     1801 ggcgcctccg atgggaagat tcgtgtagac cgctcttccc acgaaattca tattttcgat
     1861 tttattgaac atcgggagca ccttggaacc ggcgtttgag agcaccgtca gcgcatgcgc
     1921 tccgtatgcc ccggtgttca tcacgtcgct catatcgagc gcggtgatcg atccgtcgtt
     1981 ctttacgccg gtcttgatgc gaacgcgcat aggatgcctc gtcctcgccg ctgtaaaaac
     2041 ttcggaacgg ctcatctccg ccttgacgtt ccgtttgtac ttccacgccg ccagcgctgc
     2101 atagagctca aggatgatct cctgcttgct gccgaagcct ccgccgattc tgggttttat
     2161 cacgcggatc atatgcgcgg gaatacctaa gaccctgctc acggtccgtc tggcatgaaa
     2221 aggcacctgc gtagaagcca caatgacaag acgtcccttt tcgtcgaaga acgccgatgc
     2281 cgcatgcggc tccagcgcac aatgcgccgt atagtgcgtc ctgtatgttt cgtcgacgat
     2341 aaaatccgca tccgcaaacc ccttctccat gtcgccgaac gagaagagca cctctccggc
     2401 aacattttcc tccgcacgga aagggacagg gagcggcgcg tacgctccgt cttcaccgtg
     2461 aaggcttgga gaatccttat ccatagcctt ttccggatcg aagagcgtcg gaaggggagc
     2521 atactccact tttaccgcag cggcggcagc ccgcgcttcg gaaagggttc cggcgagaac
     2581 ggcagcgacg atctcccccg cgtaccgtac gcgcttgttg aaaaggcgcg tatcgtacgg
     2641 agaaggttcc gggtatcctt gccccgcggt cgtaaacaac tccgtttccg tgttgaatgc
     2701 gtgaaagacg tccgtaacgc cttccatcgc gcgagcctcg gaatcgtcga gagaaacaat
     2761 atccccgtgc gccacgggcg aataggcaaa agcaaggtag agcggatcac gcatctcgta
     2821 atcggcggtg tagcgggcag aaccgcacgc cagcgacaga gagtcaacct tgagaacggg
     2881 tttccccaca acagagaaat cagtcatgcg agctcatcct cctggaagcg agacggaccg
     2941 cttcaataat ctttacgtag ccggtgcacc ggcagtaatt tccgtcgagg gcggaatgaa
     3001 tctcctcatc ggtgggattc ggatttttgc gcaacagcgc cagcgtcgcg agaatcgcag
     3061 ccggagtaca aaaaccgcat tggacggctc cgctctccac aaaagccgtc tggatcgggt
     3121 gcggttcatg aatcgttccg atacctgcaa ccgtcagaat atctttctcc attgccgatg
     3181 cggcgaacac ctggcaagaa ttgacgagtt ttccctccag aagcaccgag caggacccgc
     3241 acactccctc gtcgcacccg cggtgcactt ccggatgacc atttgcgcga agcacgtcaa
     3301 gcaatgttgc atcaggttcg aacgtccact cattggtacg tccgttcacc aagaaagacc
     3361 ccttcatcga gacttcccct cctcag
//
LOCUS       DAOTIL010000210         3380 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminobacteriaceae bacterium isolate AMR_MDS_3026
            NODE_33253_length_3380_cov_3.324511, whole genome shotgun sequence.
ACCESSION   DAOTIL010000210 DAOTIL010000000
VERSION     DAOTIL010000210.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868050
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminobacteriaceae bacterium (wastewater metagenome)
  ORGANISM  Aminobacteriaceae bacterium
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae.
REFERENCE   1  (bases 1 to 3380)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3380)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:19:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,224
            CDSs (total)                      :: 3,198
            Genes (coding)                    :: 3,184
            CDSs (with protein)               :: 3,184
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3380
                     /organism="Aminobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_33253_length_3380_cov_3.324511"
                     /isolate="AMR_MDS_3026"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:3034609"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            445..1114
                     /gene="prxU"
                     /locus_tag="P5201_05520"
                     /pseudo
     CDS             445..1114
                     /gene="prxU"
                     /locus_tag="P5201_05520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013048998.1"
                     /note="Most members of this family contain a
                     selenocysteine.; frameshifted; internal stop; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology.
                     GO_function: GO:0051920 - peroxiredoxin activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-dependent peroxiredoxin"
     gene            complement(1178..>3380)
                     /locus_tag="P5201_05525"
     CDS             complement(1178..>3380)
                     /locus_tag="P5201_05525"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRV98027.1"
                     /translation="VRLGLRHICCFLMLCYVLLVSPSPSAGSAVPQLLPEGETASEDI
                     ADRTLPEAATEVLSGDVSVPRAASEDIEKAEAGWGTLSVSLLPEGARTAGARWSADGG
                     KTWRASGEKILLANADTTVTFKSLVGWKTPETIPVRISNESGAQAAATYVQLTGSIRA
                     EIDGPEGARWSINGKGEFLSGNMREGLVPGDYTVSFSDVVGWLKPENASVSVSSGATA
                     AVSAAYVRTGSVRVTINGPAEARWTIGEKINLPSGTLIEGLAPGEYTISFSEVALWNK
                     PEDLTITLASGALLRAGASYAAKNGSGNTKQTAHAEVETTDLGSTNTGEMIVKPQEDD
                     AVASSEEETPKEATAPEKTPVTEDDDQEQPAPEEPEPETDVDETITDVDVDEEEKEQD
                     QDQDQDDDEEDTDDSDETDSDLITNPGPSPETEPTPEPTPEPTPEPETDPVPPEVVRP
                     TSFIPELTSAERTQISRNIAAKRQSLDLGDMRYAAFSAFIPGPGSSLQPSSRPANEQA
                     DIHAQLADLITKDAEGNDALPFLLQTESFELETGATNLRGKFLVVRITFTVTHSDLAS
                     ADPKIVSRIEKGVASGRSLGDAFLDELRIFKWIGEGDNARCFDLAESVRAGGYSVNTF
                     FEAQAVKNPAEEIFDTKAQDASYTVAFTLLIFDGVTNDLSRLVQPLEGAGIIVFDGLK
                     DGKYADPLILAAPRPKNAQTQGTSGCATSAGGISPALLLLPGALLLLRRRK"
BASE COUNT          642 a          947 c          991 g          800 t
ORIGIN      
        1 cctccagtgt aggggccgac gacgatgccc gccagaatat acccgaggat gagcggctga
       61 ttcagccgtc tggcgatgag tccgccgacg agccccgcga gaacgacgag aataagatcc
      121 gatgcgatgc ccatgcgatc tccttctttc cggtttttct tgctcctatt tttgcatcct
      181 cggagaaaaa gtgcaaagca tttcttgcgt ccactggtat gcggtgtaaa aagacaataa
      241 aaaaccttga cttttaaatg acttcccata tactaataag caaataaaag aggcgtgcgt
      301 gtttcaagaa tgtttttttg ccggaagctt gtttttatct cttgttgcgt ctcttgtaat
      361 gttttgcttc ccggtatgaa aggaggagtt ctcatgagcg aggaaaaggt aggatgtacg
      421 gttcccgctg cggaggtcga aggaatgggc gccgaggctg cgcccgccga agaggtgaaa
      481 ggaggctgtt cgaatgggtg ttacggttgg catgaaggcg cctgatttca ctgcgccggc
      541 gttttacaag ggcggattca ccacggtgaa gctgtcgcag tttgcgggaa agtgggtttc
      601 cctctgcttc tatccgggcg acttcacctt cgtctgagcg actgaagtgt cggcagttgc
      661 cgaccgttat gcggagtttc aggcattggg cgtggaggtt atctccatga gcgtggacag
      721 tcagttcgtc cacaaggtat ggaacgacgt cgagctctcc aagatgatcg aggcgggcgg
      781 agttcccttc cacatgggct ccgacggcgg cggcgccatc ggaaccgtct acggcgtgta
      841 cagccccgct cagggcgtcg atttcagagg gcgcttcctc atcgacccgg acggtgtgat
      901 ccaggcgatg gaaattctca ctcctcccgt gggcaggaac gtcgacgaga cgctgcgcca
      961 ggttcaggcg ttccagcttg tgcgcaagac cggcggcaaa gaggttactc cctccggatg
     1021 gacgccgggc ggcaaggtgc tcaaccccgg tcccgacctg gtggcgaacg tctggaaagt
     1081 gtggaatccc aaggacgtct acggcaagaa gtaagccgtt tggaatagag cgacaaaaaa
     1141 gcgccccttc atcaagaagg ggcgcttttt cagctgctta ttttcttcgc cgcagcagga
     1201 gaagagctcc cggaaggagc aggagggccg gcgagattcc tcccgcggat gtagcgcatc
     1261 cgcttgttcc ctgtgtctgc gcgttcttcg ggcgcggcgc cgcgagaatg agcggatcgg
     1321 cgtatttccc gtccttcagg ccgtcgaaca cgataatgcc ggcgccctca agcggttgga
     1381 ctagtctcga gaggtcgttc gtgacgccgt cgaagatgag gagcgtgaac gcgaccgtat
     1441 aggaggcgtc ctgtgccttc gtgtcgaaga tttcttcggc ggggttcttc acggcttgtg
     1501 cctcgaagaa tgtgttgacg gagtatccgc ccgcccggac gctctctgcc aaatcaaagc
     1561 agcgcgcgtt gtccccttcc cctatccatt tgaagatgcg gagttcgtca agaaacgcgt
     1621 cgcccaatga tctccctgag gcgacaccct tttcgatcct gctcacgatc ttcggatcgg
     1681 ccgaggcaag gtcggagtgg gtcactgtaa aggtaatcct cacgacgagg aacttccccc
     1741 tgaggttcgt agctccggtc tcaagttcga aactctcggt ctgcagcagg aaggggagcg
     1801 cgtcgttccc ctcggcgtct tttgtaatca agtcggcgag ctgagcatgg atatcggcct
     1861 gttcatttgc cggccgggaa ctcggttgaa gcgaactgcc ggggccgggg ataaatgctg
     1921 aaaacgccgc ataccgcatg tcgcccagat cgagagactg tctttttgcc gcgatatttc
     1981 gggaaatttg cgtcctctct gcgcttgtta attcaggaat aaaggatgtc ggcctgacaa
     2041 cctccggagg aaccggatct gtctcaggtt cgggtgtggg ttcgggcgtg ggttccgggg
     2101 taggttctgt ctccggagaa gggcccggat tggtaatcag gtcgctgtcc gtctcgtcag
     2161 agtcgtccgt atcctcttca tcatcgtctt gatcttgatc ttgatcttgt tctttttcct
     2221 cttcatctac atcgacgtcg gtgattgttt catcgacatc agtctccggt tcgggctctt
     2281 ccggtgcagg ttgctcctga tcgtcgtctt cggttacggg cgtcttttcc ggagcagttg
     2341 cctccttcgg tgtttcttcc tcggatgacg caaccgcgtc atcttcctgt ggttttacga
     2401 tcatctcgcc ggtattcgtc gaaccaaggt ccgtcgtttc gacctcggca tgtgcggtct
     2461 gttttgtgtt tccgctcccg ttctttgcgg cgtaagaggc tccagcgcgc agaagtgctc
     2521 cggaagcgag cgtaatcgtc agatcctccg gtttgttcca tagcgctact tccgagaagg
     2581 aaattgtgta ctctccggga gcgagtcctt cgatgagcgt gccgctcggg aggttgattt
     2641 tttccccgat ggtccatctg gcttcagcag gtccattgat tgtgacgcgg acggaacccg
     2701 tacggacata ggctgccgat acggctgctg tggcgcctga actgacggag acgctcgcgt
     2761 tctccggttt cagccacccc acgacgtccg aaaacgagac ggtatagtcc ccgggaacaa
     2821 gcccttcgcg catatttccg ctgaggaact cgcctttgcc gttgatactc catcgcgctc
     2881 cctccggtcc gtcgatttcc gcccggatgg aacctgtgag ctgaacgtat gtcgcggctg
     2941 cctgcgcacc gctctcgttg ctgatcctta cgggaatagt ctccggagtc ttccagccga
     3001 cgaggctctt gaaggtgacc gtcgtgtcgg cgttggcaag gagaattttt tccccggaag
     3061 ctctccatgt tttgcctccg tcggcgctcc agcgcgcccc cgcagtccgc gcgccttcag
     3121 gcagcagcga aacggaaagc gttccccacc ccgcttcggc tttttcgata tcctcgcttg
     3181 ctgcccgcgg aacggaaaca tctcctgaga gaacctccgt tgcggcctcc ggaagagtac
     3241 gatcggcgat atcctcgctc gccgtctctc cttcgggcag cagctgcgga acggccgaac
     3301 ccgcggacgg agagggtgaa accaacaaaa cataacagag catcaaaaaa caacaaatgt
     3361 gccgcaagcc cagcctcacg
//
LOCUS       DAOTIL010000211         3376 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminobacteriaceae bacterium isolate AMR_MDS_3026
            NODE_33304_length_3376_cov_5.451069, whole genome shotgun sequence.
ACCESSION   DAOTIL010000211 DAOTIL010000000
VERSION     DAOTIL010000211.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868050
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminobacteriaceae bacterium (wastewater metagenome)
  ORGANISM  Aminobacteriaceae bacterium
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae.
REFERENCE   1  (bases 1 to 3376)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3376)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:19:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,224
            CDSs (total)                      :: 3,198
            Genes (coding)                    :: 3,184
            CDSs (with protein)               :: 3,184
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3376
                     /organism="Aminobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_33304_length_3376_cov_5.451069"
                     /isolate="AMR_MDS_3026"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:3034609"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(161..961)
                     /locus_tag="P5201_05530"
     CDS             complement(161..961)
                     /locus_tag="P5201_05530"
                     /inference="COORDINATES: protein motif:HMM:NF012234.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HRV98028.1"
                     /translation="MIESFFGLHGIPFGRSVPVAELLRTDGWDELHARMLHTARARGF
                     GVFTGDTGTGKTTSLRRFAGELDPNRYRVLYVCDSFLTPRNFYWEVLHQLGCTPRFYR
                     GDAKRQFQKALREVNEERKNPVVVVDEAHLLSREMLEEIRFLLNTGMDSESSLALLLV
                     GQSELKDALKLQVNQAIAGRVDIRFHLGAMDKGQTASYVKRHMDSVKAPRDIFTSTAL
                     GMIHEYAGGIPRKVNKVASSCLMTAAVRKETLVDDHLVQEVLESEFEI"
     gene            complement(954..2354)
                     /locus_tag="P5201_05535"
     CDS             complement(954..2354)
                     /locus_tag="P5201_05535"
                     /inference="COORDINATES: protein motif:HMM:NF012870.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DDE-type integrase/transposase/recombinase"
                     /protein_id="HRV98029.1"
                     /translation="MKEIELDGDREEGLRRFALVAPLLEEGLSSSEATERRLRILERE
                     EISERTLRRWVAAYRSGGFEGLVKKPRKDKGTCRAISPEALALAEECRRELPRRSAGL
                     IRDWLGTQGHEVARSTLERHLRANGLSGRDLRAEREAKSGSRRFVRIGRNSLWQADLK
                     YGPYIPDPEHPGRKRRTYLLAILDDATRKVTHAEFYDNQKLPVLEDAMKKAIQRHGSP
                     KRIYVDNGKIFVSTWMKLACAKLNIKHINASPYNPEGKGKIERFNRTVEEFLAEYALQ
                     EAGTLEGLNSFFRSWLSERYQHKAHGALNGKTPAEMFAEDATPLRFHSLETLRDAFLH
                     EGERTVDKSGCLKLEGDLYDAGTDYLRKKVTIRYDPFDREIVQLWYKGTRRSDIRKAR
                     IGESNATLKAPAEKLDAGGESRVLNTYREEHRKRFVKTLGAYGLRNAGKSGESRAGEH
                     AAGEERSGSGEEKLRD"
     gene            complement(2412..2903)
                     /locus_tag="P5201_05540"
     CDS             complement(2412..2903)
                     /locus_tag="P5201_05540"
                     /inference="COORDINATES: protein motif:HMM:NF040103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6431 domain-containing protein"
                     /protein_id="HRV98030.1"
                     /translation="MPESCFFCEGPFRFHGFRKRRCILLGEKRPFFVKRFRCCVCRKT
                     FTVLKKFMLPFKQHAASEIEEALSGEKESGTHADERTIGRWRKAFHTVLPALCALLED
                     LAEAVFGSKVSLSASPESPLKRLRRASETLGTYLPGESALARAMFLESRHRLCLGWQS
                     FRL"
     gene            complement(3058..>3376)
                     /locus_tag="P5201_05545"
     CDS             complement(3058..>3376)
                     /locus_tag="P5201_05545"
                     /inference="COORDINATES: protein motif:HMM:NF024586.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HRV98031.1"
                     /translation="IRDENGAPKCIAASAVDMTAFYHAEQNYRTLFREMLNGFALHEM
                     LYDQSGKAVDYRFLEVNPAFERLTGLKAESVVGRTVLEVLPGTERHWIETFGVSARSF
                     RPE"
BASE COUNT          591 a         1110 c          938 g          737 t
ORIGIN      
        1 gcatggtcgc cttggagact gcggcgtact tgcggtagat cttgcagaac tgtgctgtag
       61 cggcttgatc tccttgtccg gggtcgactc tttttcgatg accgcggaga cagctttcca
      121 ttcgtgtccg taaggggcgg aaggataccg cccctccggc tcaaatttcg aattcgctct
      181 ccaaaacctc ctgcacaaga tggtcgtcca caagagtctc tttcctgacc gccgcagtca
      241 tcagacacga cgacgcgact ttattcacct tccgcggaat tcctccggcg tactcgtgga
      301 tcattccaag cgccgtcgac gtaaaaatgt ctctgggggc tttcaccgag tccatgtgcc
      361 gtttgacgta cgaagcggtc tgccctttgt ccatggctcc cagatggaag cggatatcca
      421 cccttcccgc tatggcctgg ttcacctgga gtttcagcgc gtccttcagt tcgctttgtc
      481 ccaccagcaa gagcgccagc gagctctcgg agtccattcc ggtgttgagg agaaaacgga
      541 tctcctcgag catttcgcgg gagagcagat gcgcctcgtc caccacgacc accgggttct
      601 tcctctcctc gttcacttct ctcagagcct tctggaactg ccgcttcgcg tctccgcgat
      661 agaagcgtgg agtgcaaccg agctggtgca gcacctccca gtagaagttg cggggagtca
      721 ggaaggaatc gcagacgtac agcacgcggt atcggttggg gtccagttcg cccgcgaagc
      781 gtctgagcga cgtcgtcttc cccgttcccg tatctccggt gaagaccccg aatcctctgg
      841 cgcgggcggt gtgaagcatc cgggcgtgca gttcgtccca gccgtccgtc cgcaggagtt
      901 cggcgacggg aacgctgcgg ccgaagggga ttccatgaag tccgaagaac gactcaatca
      961 cggagcttct cctctccgct tccggagcgc tcttctccgg cggcatgttc ccctgcgcgg
     1021 ctttccccgc ttttcccggc gttcctcagg ccgtacgcac cgagcgtctt cacgaaacgc
     1081 ttgcggtgct cctccctgta ggtgttcagc acacgggatt ctcctcccgc gtccagcttc
     1141 tcggcgggtg ccttcagcgt cgcgttgctc tctccgatgc gggccttccg gatgtcgctc
     1201 cgccgcgttc ccttgtacca gagctggacg atctccctgt cgaacgggtc gtagcggatc
     1261 gtcaccttct tgcgcagata gtccgtcccg gcgtcgtaga gatcgccttc cagcttcagg
     1321 cagccgcttt tgtccaccgt ccgctcgccc tcgtggagaa atgcgtcccg cagcgtctcc
     1381 aagctgtgga atcgaagcgg cgtcgcgtcc tccgcgaaca tctccgcggg cgtctttccg
     1441 ttcaatgcac cgtgcgcctt gtgctggtag cgttccgaca gccaggaacg gaagaacgaa
     1501 ttcagcccct cgagcgtccc cgcctcctga agcgcgtatt ccgccagaaa ctcctcgacc
     1561 gtcctgttga aacgttcaat tttgcccttt ccctccggat tgtacggcga ggcgttgatg
     1621 tgctttatgt tcagcttcgc gcacgccagc ttcatccacg tcgagacgaa gatctttccg
     1681 ttgtcgacgt agatgcgctt cggcgagccg tgtctctgga tcgccttctt catcgcgtcc
     1741 tccaacacgg gaagtttctg gttgtcgtag aactccgcgt gcgtcacctt cctggtggcg
     1801 tcgtcgagga tggccagaag atacgttcgc cgctttcttc cgggatgctc cgggtcgggg
     1861 atgtacggtc cgtatttcaa atccgcctgc cacagactgt ttcttccgat cctgacgaaa
     1921 cgcctgctcc ccgatttcgc ctccctctcc gcgcgcaggt ctcttccgga gagtccgttg
     1981 gcccgaagat gccgctccag cgtcgaccgg gcgacctcgt gcccctgcgt acccagccag
     2041 tcgcggataa gacccgcgct ccgccgggga agttccctgc ggcattcctc cgcgagagcg
     2101 agcgcctccg gcgatatggc tctgcacgtc cccttgtcct tgcgcggctt cttcaccagc
     2161 ccttcgaagc ccccgctccg gtacgccgcg acccatctcc tcaacgtccg ctccgaaatc
     2221 tcctcgcgct ccagaatcct cagacgccgc tccgtggctt ccgaagacga caacccctcc
     2281 tccagcaacg gcgccaccag agcgaatctc cggagccctt cctctctgtc gccgtccagt
     2341 tcgatttcct tcatccccgc cacgtccttc ctctccgttt tttctcctcg tccgggagca
     2401 cggaacggag tctacaggcg gaaggattgc catccaaggc aaagtcggtg gcgggattcg
     2461 agaaacatcg cccgcgccag agcggattca ccgggaagat acgtccccag cgtctccgac
     2521 gcgcggcgca gacgcttcaa tggagattcc ggcgacgcgg acagggaaac cttcgaaccg
     2581 aacaccgctt ccgcgagatc ctccaaaagg gcgcaaagag cgggaagaac cgtgtgaaaa
     2641 gccttcctcc aacgtccgat ggtgcgctcg tccgcatgcg tcccgctctc cttttctccg
     2701 gaaagggcct cttcgatctc ggaagcggca tgctgcttga aggggagcat gaacttcttg
     2761 aggacggtga acgtcttccg gcagacgcag caacggaagc gcttcacgaa gaacggacgc
     2821 ttctctccga gaagaatgca gcgtcgcttg cggaagccgt ggaaacgaaa cgggccttcg
     2881 cagaaaaaac aggactcggg caggggaaaa tcttcgttgt cttctggtat aattcgaatg
     2941 gtttttcacg atagtccccc ggttcgatcc tgtcttggcg gagaggtcgt ccgggggact
     3001 ctcctttctc ttcagcctgt gtatacagat taccccggac ggactcccat gtaaagccta
     3061 ttccggtcga aaagaccggg ccgaaacacc gaacgtctcg atccagtgcc gttcggtgcc
     3121 gggcagaacc tccagcacag ttcgccccac aacggactcg gccttcaggc cggtcagccg
     3181 ttcgaaggcg gggttgacct ccagaaagcg atagtcgacg gccttgcccg attgatcgta
     3241 gagcatctcg tgcagggcga agccgttcaa catctcgcgg aagagcgtcc ggtagttctg
     3301 ttccgcatgg tagaacgcag tcatgtcgac ggcggaggcg gcgatgcact tcggcgcacc
     3361 gttctcgtcc cgtatt
//
LOCUS       DAOTIL010000212         3375 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminobacteriaceae bacterium isolate AMR_MDS_3026
            NODE_33330_length_3375_cov_3.141566, whole genome shotgun sequence.
ACCESSION   DAOTIL010000212 DAOTIL010000000
VERSION     DAOTIL010000212.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868050
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminobacteriaceae bacterium (wastewater metagenome)
  ORGANISM  Aminobacteriaceae bacterium
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae.
REFERENCE   1  (bases 1 to 3375)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3375)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:19:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,224
            CDSs (total)                      :: 3,198
            Genes (coding)                    :: 3,184
            CDSs (with protein)               :: 3,184
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3375
                     /organism="Aminobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_33330_length_3375_cov_3.141566"
                     /isolate="AMR_MDS_3026"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:3034609"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1374)
                     /locus_tag="P5201_05550"
     CDS             complement(<1..1374)
                     /locus_tag="P5201_05550"
                     /inference="COORDINATES: protein motif:HMM:NF024583.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain-containing protein"
                     /protein_id="HRV98032.1"
                     /translation="MMPKDTVSLDQVFISSPVVVLWGAPLEGRPVEFVTKNVSQFGYA
                     AGDLMTGSVTFGAILHEEDRERCFALFSDALFGREDEIRMEYRIVAPNGDVRWVEDRT
                     VFHREPAGEAVAYQSSLLDITGRKLSEMRLREAEVRLARVLEASKISVWDYFPDKDAT
                     FATGRAILYGDAENEALLVPRKALWDKLVPPQLRPLLEEKLRQLLSGTVDSAEQEIDI
                     PGPGDTKRRAVSKAFPVRDAQGNVVRIAGLNIDVTKLKETERRIARQNKRLELFHATF
                     LVLMEETDTEKLLGRILEKATELARTEHGRISLLDTEKNVFRLVLGRGFLKAMEGETR
                     PSDAGLAGEVLRQKKCVVIKEYPSFYGKIDDPRLARLTTVIGMPLFQGDVFFGVLSVA
                     YWDVFPEVDEEILEDLDQFAGAVSIALENARLYDSARRELKERVFAGERLRFHARLVD
                     AAARASGV"
     gene            complement(1399..>3375)
                     /locus_tag="P5201_05555"
     CDS             complement(1399..>3375)
                     /locus_tag="P5201_05555"
                     /inference="COORDINATES: protein motif:HMM:NF024583.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HRV98033.1"
                     /translation="LPESDELLGDGRASLFGISDDAIPKSRKELWDAIVPPAGRERLE
                     NAWREILEGTTDHLDLEHKLIKVDGSAVWVLTKGFPVRGEDGGLLRMQGVTVDISPLK
                     QAEEKVVQQNERLALLHEISLAFMEELDSESLIRRILEKAVELAGSANGLIGVIEEDG
                     ENIRNTWGVGLYEKLVGNLVPVSVGLIGEVLKKKSRVIVEDYRTYPERMELDAYTNVT
                     TVLGLPLYRGERLMGAVAIAFTDTVRFLEEPLLNLLDQFAAAASIALENARFYEDARK
                     EVEERERTEQRLRFHQALVEAAAEGASFLLSIESEEQAMFFALRALGQAAAADSIALF
                     RNSEGHEGAVAVIIAKAPISGNEEFFILPDHLPWGDEYSSIYESLSDGMPFHGTIASI
                     ASERHDASFATITMIPVFIRSYFWGVLAFCFQRERPPFETDEIDVLRAAAYNMAASVI
                     RWESEREVKKGYEKLRRTFTDVIRTMGQIVGKKDPYTIEHQERVAALALEIGNRLGLG
                     KERCEGLRIAGLVHDIGKIEIPGEILSKPGRLSPIEFELIKTHARSGYDILREVDFPW
                     PVAEIAHQHHERMNGSGYPRGLKGEEILLEARIITVADVVESMVSHRPYRPSLGINAA
                     REEIAEKSGVLYDSAVVDACLSLLDERPDLIAEL"
BASE COUNT          730 a         1065 c          852 g          728 t
ORIGIN      
        1 cacgccggag gcccgggctg cggcatctac gagacgagca tgaaagcgaa ggcgttctcc
       61 cgcgaacacc cgctccttca gctcccgccg ggcgctgtca tagaggcgag cattctccag
      121 tgcgatcgag acggccccgg caaactgatc gaggtcctca aggatctcct cgtctacctc
      181 gggaaacaca tcccaatagg cgaccgagag cacgccgaaa aaaacatccc cctggaagag
      241 aggcattcct atcactgtgg tgagcctcgc aaggcgagga tcgtctatct tgccgtaaaa
      301 ggatggatac tctttgataa ccacacattt tttctgccgc agcacctctc ctgcaagtcc
      361 ggcgtcgctc ggccgggtct ccccctccat ggccttcaga aatcctcgcc ccagaacaag
      421 ccggaagaca tttttttcag tatccaggag agaaatcctt ccatgttccg tacgcgcgag
      481 ctccgtggcc ttctccagaa tacgtccgag cagtttttcc gtatccgtct cttccatgag
      541 cacaaggaac gtggcgtgaa acagctcaag acgtttgttc tgacgagcga tcctccgttc
      601 ggtctccttc aatttggtca cgtcgatatt caaaccggca atacgaacaa cgtttccttg
      661 cgcgtcccga accggaaacg ccttcgaaac tgcgcggcgc ttcgtatcgc cgggacccgg
      721 gatatcaatc tcttgctcgg cggaatctac tgttcctgag agcaactggc gcagtttttc
      781 ctcgagcagg gggcgaagtt gcggaggaac gagcttgtcc caaagcgcct ttctgggaac
      841 cagaagcgcc tcgttttctg cgtccccgta gagaatagcc cgtccggttg cgaacgtcgc
      901 gtccttgtcg gggaagtagt cccacacgct gattttcgag gcctccagga cgcgggccaa
      961 acggacctcg gcctccctga ggcgcatctc cgacagtttt ctccctgtga tgtccagaag
     1021 gctcgactga tacgcaacag cctctcccgc cggctcacgg tgaaaaacag tccggtcctc
     1081 tacccagcgc acatctccgt tcggcgcaac gatacggtat tccatccgga tctcgtcttc
     1141 ccgcccgaaa agagcgtcgg aaaacagcgc gaagcaacgt tcccggtcct cctcgtgaag
     1201 aatggcgccg aacgtcacgc tcccggtcat caaatccccg gccgcatacc cgaactggga
     1261 gacattcttc gtcacaaatt cgacgggacg cccttccagc ggagcgcccc acagaacaac
     1321 aaccgggctg gagatgaaga cttgatcgag actgacggta tccttcggca tcatggcagt
     1381 tcgtccgctc atgcgctgct acagttcggc gatgagatca ggacgttcgt cgagaagcga
     1441 cagacatgcg tcgacaacgg cggaatcgta caggacgccg cttttttccg ctatttcttc
     1501 ccgggcggcg ttgattccaa gagaggggcg gtacggacga tgcgacacca tggactctac
     1561 aacatcggcg acggtgatga tacgagcctc cagaagaatc tcctcgcctt tcagccctct
     1621 cgggtacccc gatccgttca tcctctcgtg atgctgatgc gcgatctcgg ctacaggcca
     1681 ggggaagtcc acctcccgca gaatgtcgta cccggaacgc gcgtgcgtct tgatcagttc
     1741 aaactctata ggggagagtc ttccgggctt gcttaaaatc tcccccggta tttcgatctt
     1801 gccgatatcg tgcaccaatc cggcgatgcg caatccttcg cagcgctcct ttcccagtcc
     1861 caatctgttc ccgatctcca gggcgagcgc ggcgacgcgc tcctgatgct cgatagtata
     1921 ggggtccttc ttcccgacaa tctgtcccat cgtccggatg acgtccgtaa acgtccgccg
     1981 gagtttttca taccccttct tcacctctcg ttcggactcc cagcgaatca ccgatgcagc
     2041 catgttatac gcggccgcgc gaagcacgtc gatctcgtcc gtctcgaaag gcggccgctc
     2101 ccgctggaaa cagaacgcaa gcactcccca gaaatacgaa cggatgaaca cgggaatcat
     2161 tgtgatcgta gcaaaagaag cgtcatgccg ctcggaagca atggaagcga tcgttccgtg
     2221 aaaaggcatt ccatccgaaa gagattcata aatggaagaa tactcatccc cccagggaag
     2281 atgatccggt aaaatgaaga actcttcgtt ccctgaaatc ggcgctttgg ctataattac
     2341 cgccactgcg ccctcatgac cttcgctatt gcggaaaagt gcgatgctgt cggcagccgc
     2401 agcctggccg agagcgcgca gggcgaagaa catggcctgt tcctcggatt cgatggacag
     2461 aagaaagctt gccccctccg cagccgcttc cacaagcgcc tggtgaaaac gcagccgttg
     2521 ctcggttcgt tcccgctcct ccacttcctt tcgggcatcc tcatagaatc tggcattctc
     2581 cagcgctatg gaggcagcgg ccgcaaactg gtccaggaga ttcagcaggg gttcctccag
     2641 aaatcgaacc gtatcggtaa aagctattgc aacagctccc atcaatcgct ctccccgata
     2701 cagggggaga ccaagcaccg tcgttacatt cgtgtacgca tccagttcca ttcgttccgg
     2761 atacgtccgg taatcttcta caatcaccct gctctttttt ttcaggactt cgccgatcag
     2821 gccgactgaa accggaacca ggtttcctac taacttctcg taaagaccga cgccccaggt
     2881 atttcgtata ttttccccgt cttcttctat gacgccgata agaccgttcg ccgaacctgc
     2941 gagttccacg gccttttcga gaatcctccg gataagcgac tccgaatcca gttcctccat
     3001 gaatgccagc gaaatttcgt gcagaagagc aagccgttcg ttttgctgaa cgaccttctc
     3061 ctccgcctgt ttgagagggg agatgtcaac ggtaacccct tgcatgcgaa gcagtcctcc
     3121 gtcttcaccc ctgacaggaa accccttcgt cagaacccat actgccgagc cgtcgacctt
     3181 gatgagcttg tgctccaggt cgagatgatc cgttgtcccc tccaggatct ccctccatgc
     3241 gttttcaagc cgctcgcgtc cggcgggcgg aacaatggcg tcccataact ctttccggga
     3301 cttcggaatc gcatcatcgc tgatgccgaa gagagaggct ctcccgtccc caagcagttc
     3361 atcgctttcg ggaag
//
LOCUS       DAOTIL010000213         3372 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminobacteriaceae bacterium isolate AMR_MDS_3026
            NODE_33359_length_3372_cov_3.758818, whole genome shotgun sequence.
ACCESSION   DAOTIL010000213 DAOTIL010000000
VERSION     DAOTIL010000213.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868050
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminobacteriaceae bacterium (wastewater metagenome)
  ORGANISM  Aminobacteriaceae bacterium
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae.
REFERENCE   1  (bases 1 to 3372)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3372)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:19:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,224
            CDSs (total)                      :: 3,198
            Genes (coding)                    :: 3,184
            CDSs (with protein)               :: 3,184
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3372
                     /organism="Aminobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_33359_length_3372_cov_3.758818"
                     /isolate="AMR_MDS_3026"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:3034609"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..549)
                     /locus_tag="P5201_05560"
     CDS             complement(<1..549)
                     /locus_tag="P5201_05560"
                     /inference="COORDINATES: protein motif:HMM:NF013757.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IclR family transcriptional regulator C-terminal
                     domain-containing protein"
                     /protein_id="HRV98034.1"
                     /translation="MGSLDKAMSILKHLGDPPYEFSLSELASFVDIGKSGAFKILKTL
                     QAHNFVIQDFLTKKYHLSPIMIRLGNVYGRLKGIEEITRPVLSYISRTLGETTYISVW
                     EGDRAFPAYKNVVRGGCYDAADFIGTSIPLNSGGSAMVLCAYQDGELMRKILSTAELE
                     KRTPFTIMDREGLLEEYEKIRER"
     gene            complement(791..1303)
                     /locus_tag="P5201_05565"
     CDS             complement(791..1303)
                     /locus_tag="P5201_05565"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRV98035.1"
                     /translation="MKQKGIVLLFRDIRVIFNRTFLLRRIFMLIRFVWRQCYYELIPE
                     ALRKYGVDAFFIEAAFEEAEVSVFPSMDPESVSESKSLGLHCLWNGEKVLARSYCFLA
                     ARDLSYIFPVSPAFPFDHLGSPGVFEPLDPVVSVDSLRSIRFLSMSADDGEEMEEEDF
                     YAHELDGESE"
     gene            1432..1521
                     /locus_tag="P5201_05570"
     tRNA            1432..1521
                     /locus_tag="P5201_05570"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1466..1468,aa:Ser,seq:cga)
     gene            1589..1676
                     /locus_tag="P5201_05575"
     tRNA            1589..1676
                     /locus_tag="P5201_05575"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1623..1625,aa:Ser,seq:gga)
     gene            2331..3335
                     /locus_tag="P5201_05580"
     CDS             2331..3335
                     /locus_tag="P5201_05580"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRV98036.1"
                     /translation="MPKNRADMLAGYFLESLDETARATMRFLEQEPPVEEPSEDEENR
                     KKKKERKLESFPKLMEGIRRLKTIAKDEDGSDLVVDRLLSSIDMLFLESLTAYAYKKS
                     ALEVVRRKKKLRQEVNDTPFSPEQALLEIMKQHLERIYAVQERACKAFSEENAPEKSG
                     YTPFLLPDSVEYLDILDEEEIQREKKKKAGDLSFERAFLEILSADRGLFTLPRIRSRG
                     LPGILFLPGNAVGAYDKKADFFLLPFSALRERAFEHLLNAFASFRWAWDDSGKVLNLC
                     ADLVGSRGAKPQLELEAAFRRAYSSWLQSEIRGRAVDEHGESIKKALRPEGNFARFGE
                     "
BASE COUNT          875 a          845 c          820 g          832 t
ORIGIN      
        1 cctctcccgg atcttttcgt actcttccag caacccctcc ctgtccataa tcgtgaaggg
       61 cgttcttttc tccagctctg ccgtactcag aattttcctc atcagctccc catcctggta
      121 tgcgcagaga accatcgccg atcctccgga attcagaggg atgcttgtcc cgataaaatc
      181 tgcggcatcg tagcagccgc cccgcacgac gtttttatag gccggaaacg cacgatcgcc
      241 ttcccagacg gaaatatagg tagtctctcc aagcgttcgg gaaatgtatg aaagaacagg
      301 ccgggttatc tcttcaatcc ccttcaggcg tccgtatacg ttgccgaggc ggatcatgat
      361 cgggctgaga tggtattttt tcgtaagaaa atcctggatg acaaaattat gggcctggag
      421 ggttttcagg attttaaaag cgccgctttt cccgatatca acaaaagagg ctagctctga
      481 aaggctgaat tcgtaagggg gatctccgag gtgcttcaat attgacatgg ctttatcgag
      541 actgcccatt acgacctcct tttccctatg ggaaatcatt ttcccaaaat atttacaagt
      601 catactatat cagcaaaagc actgtttgtc gatattcaaa aaaacggtca caaacggtgg
      661 tatcagggag cagtcattct tcggcaataa aaaaggcggc cataggccgc cgctgaaaag
      721 gatctgatcc gggatccagc tctgcattca ggacacatcg cgtgaaagat cagcactttt
      781 tctctttaag ctactcactc tcaccgtcga gctcgtgagc ataaaaatct tcctcttcca
      841 tttcttcccc gtcatccgca gacatggaaa gaaacctgat agatcgaagg ctgtccacgc
      901 tgacaacagg atcaaggggt tcaaacaccc ccggactccc caggtggtcg aaggggaacg
      961 cgggagaaac cggaaaaatg taagacaggt cccgggccgc aaggaaacag taagaacgtg
     1021 ccaatacctt ctcgccgttc caaaggcaat ggagtccaag actttttgat tccgaaacgg
     1081 attccggatc catagagggg aaaacggata cctcggcctc ttcgaaggca gcctcgataa
     1141 aaaaagcgtc aaccccgtat ttcctgagag cctccggaat aagctcatag taacattgcc
     1201 gccatacaaa tcggatgagc ataaaaatcc tccgcaaaag aaatgtgcgg ttaaaaataa
     1261 cacgaatatc gcggaataac aatactattc ccttctgttt caccttttcc cctctgtcaa
     1321 ttctcttttc cccctcgctc tctcctcgga tttccaggaa acacagcggg aaaaaaggct
     1381 gcgggcatac ttggcaagcc ggaaatgaac gtgtataata cctttccgtg cggagaagtg
     1441 cccgagtggt cgaaggggat tgactcgaaa tcaattgggc ggcttgctgc cccgtgggtt
     1501 cgaatcccac cttctccgcc agcaataaat cggatttcca cgtatggcaa tcctgaaaag
     1561 ctgtgatata atgttcgccg ttgcatgcgg agaagtggcc gagtggtcga aggcgggcgc
     1621 ttggagagcg tctgaaccgt gaggttccgt gggttcaaat ccctccttct ccgccatttt
     1681 tttcaatatc ttccccccta ataaatcttt ctcattcact taaaaaacga atgtcttgtc
     1741 tgttcgcctg ctctgatgta tcctcttacc agagacgtac cttagagatc ggagatgttt
     1801 cagatgcaga caaaaacaac tctttcctcc gggagctttt ttatttcgcg aactagggcc
     1861 gatatatgct cggcatgatt ggttcctccg aatcgctacc tgaagagcag cgtactacat
     1921 tgactgccct tgcccatttc cacaaaacaa gggttctttc cggcttgccc tctcctcaac
     1981 gtttagcgtc tctccacaga tttctcgtac gccgttttat cgatgatact acgttctcat
     2041 tgactcagga acagtatgag cttgtcgatt caggagcgta tagctctgct tcgtctctct
     2101 cttcggtgca aaggagcgag gtgtgtgcgg cgctcactct gactgaatgg attgaagaca
     2161 cgagagctgc ctttgaagaa ggaaaagaag attatgccgt ttcacacgta acgaaagcga
     2221 tcgcttcatc cgaggcacgt ttgaacgcgc tcgggggcag aattcgcaca atgctgccgc
     2281 tccttttcac aacccttggg agcgttactt ccgacggggg cctttttatc atgccgaaga
     2341 accgtgcaga tatgctcgcc gggtattttt tagaatctct tgacgaaaca gcccgggcga
     2401 caatgcgttt tctggagcag gagccgcctg tggaggagcc ttcagaggat gaagaaaaca
     2461 ggaaaaagaa gaaagagcgg aagctggaaa gctttccgaa gttgatggag ggcattcgac
     2521 ggctgaagac tattgccaaa gatgaggacg gcagcgattt ggtggtagac agattgcttt
     2581 cgagcatcga tatgttattc cttgaatcac ttactgcgta tgcctacaaa aaaagcgctt
     2641 tggaagttgt gcggcgaaaa aaaaagttac ggcaagaagt gaacgatacc cccttctcac
     2701 ctgaacaggc gcttcttgaa atcatgaagc aacatctgga gagaatctat gctgttcaag
     2761 agagagcgtg caaagcgttc tcagaggaga acgcaccgga aaaaagcggc tatactcctt
     2821 ttctcctccc tgattccgtc gagtatctcg atatactgga tgaagaagaa attcaacgtg
     2881 aaaaaaagaa aaaagcagga gatctgagct ttgaaagagc attcctcgag attctttctg
     2941 ctgacagggg gctttttact ctcccgcgaa ttcgttcccg gggtcttccc ggcattctct
     3001 ttcttcccgg aaacgctgtc ggtgcatacg ataagaaagc agatttcttc ctccttcctt
     3061 tttccgcgct cagagaacgg gcattcgagc accttttgaa tgcgtttgcc tctttccgct
     3121 gggcttggga tgacagcgga aaagtcttga acttgtgcgc tgatcttgtg ggcagcaggg
     3181 gagcgaagcc gcagctggaa ctcgaagctg cgtttcgcag agcctattcg tcatggcttc
     3241 agtctgaaat tcgtgggaga gcagtcgacg aacacggtga atcgataaaa aaagcgctca
     3301 ggccggaagg aaacttcgcg cgtttcggag agtaattgct tcagacagaa gagagagggt
     3361 gaataccccc tc
//
LOCUS       DAOTIL010000214         3371 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminobacteriaceae bacterium isolate AMR_MDS_3026
            NODE_33374_length_3371_cov_3.784982, whole genome shotgun sequence.
ACCESSION   DAOTIL010000214 DAOTIL010000000
VERSION     DAOTIL010000214.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868050
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminobacteriaceae bacterium (wastewater metagenome)
  ORGANISM  Aminobacteriaceae bacterium
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae.
REFERENCE   1  (bases 1 to 3371)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3371)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:19:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,224
            CDSs (total)                      :: 3,198
            Genes (coding)                    :: 3,184
            CDSs (with protein)               :: 3,184
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3371
                     /organism="Aminobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_33374_length_3371_cov_3.784982"
                     /isolate="AMR_MDS_3026"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:3034609"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..766
                     /locus_tag="P5201_05585"
     CDS             <1..766
                     /locus_tag="P5201_05585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008712900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="dihydrodipicolinate reductase"
                     /protein_id="HRV98037.1"
                     /translation="IDELAKKNNCTICGAGYQDVLWGNLIATLAGATHSIRTIRGKSS
                     YNVEDYGIALAQVHGAGLTLDAFEKEIASADNISEAERKALIEKGEFLPSYMWSVNGW
                     LCERLGLTPVSQTQKCVPHTHTSELHSKTLGMTIPAGHATGMSAVVTTETEEGITLET
                     ECIGKVYAPDEFDRNDWTIVGEPDTQVVINRPATVELTCATIVNRLPDIVNAPAGYVT
                     TDKMPTNSYRVKPLHTYVDAEALQSSSSKRAVWHDE"
     gene            1052..1240
                     /locus_tag="P5201_05590"
     CDS             1052..1240
                     /locus_tag="P5201_05590"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRV98038.1"
                     /translation="MIAVKDEVRTLLERLPDDCSFEDIQYHLYVLEKVRRSAARVEQD
                     GALEHDEVEKRLHRWLSV"
     gene            1225..1533
                     /locus_tag="P5201_05595"
     CDS             1225..1533
                     /locus_tag="P5201_05595"
                     /inference="COORDINATES: protein motif:HMM:NF016876.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008657 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) inhibitor activity
                     [Evidence IEA];
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA];
                     GO_process: GO:0009432 - SOS response [Evidence IEA];
                     GO_process: GO:0030541 - plasmid partitioning [Evidence
                     IEA];
                     GO_process: GO:0051290 - protein heterotetramerization
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HRV98039.1"
                     /translation="MALRLKWSPDAVEDLETIAEYIARDSSSYASSVVSKIVTVAKTI
                     PDFPRSGRIVPEIGDHAIREHVVFSYRLIYRVEPDHITLLAVIHGKRLLEKNPAWCDE
                     "
     gene            complement(1594..2436)
                     /locus_tag="P5201_05600"
     CDS             complement(1594..2436)
                     /locus_tag="P5201_05600"
                     /inference="COORDINATES: protein motif:HMM:NF017536.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pirin family protein"
                     /protein_id="HRV98040.1"
                     /translation="MNEIRSIRRIVTGKPATDGAGVKLVRVIGYNDTQDFDPFLMLDA
                     FDSTNPEDYVKGFPWHPHRGIETITYLIRGDIAHGDSLGNKGRILDGECQWMTAGSGI
                     LHQEMPQASERMLGTQLWLNLPAKDKMTEPKYNDIVRENVPTVAEEGLNIRIISGNYK
                     GTDGAFRGEYADALYLDAEMAPGAEWLLETDPEATLFIYIVQGAGFFGPSGEGVAEKH
                     AVLFEKGNAFRAKASEAGARIFLMAAKPLGEPIAWGGPIVMNTREELDLAFRELQEGT
                     FIKR"
     gene            2666..3196
                     /locus_tag="P5201_05605"
     CDS             2666..3196
                     /locus_tag="P5201_05605"
                     /inference="COORDINATES: protein motif:HMM:NF014573.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin family protein"
                     /protein_id="HRV98041.1"
                     /translation="MRYIALLGSPRKDGNTAALLASFLEGAASKGHSGELIRLHEKKI
                     LPCAGCNACKTEGKCILSDDMTALYPLVEEAEVIVHAFPVYWWSMSAQTKLFQDRLYA
                     MDYTHFKGKQLFLLTTYGGEDPNSGPRLCEQSFRDICEFLDMQFRDFIGVCSGTTPVR
                     DNKRALERAFLAGASI"
BASE COUNT          695 a         1021 c          979 g          676 t
ORIGIN      
        1 gatcgacgag ctcgcgaaaa agaacaactg cacgatttgc ggcgcggggt accaggacgt
       61 cctctggggc aacctgatcg ccacgctcgc gggagcgacg cacagcatcc gcacgatacg
      121 cggcaagtcc agctataacg tggaggacta cggcatcgcg ctcgctcagg tgcacggcgc
      181 cgggctgacg ctggacgcct tcgagaagga gatcgcctcg gccgacaata tttccgaagc
      241 tgagcgcaag gcgcttatcg agaagggaga gttccttccc tcctatatgt ggagcgtcaa
      301 cgggtggctg tgcgaacgcc tggggctgac tcccgtttcg cagacgcaga aatgcgtgcc
      361 gcacacgcac acgtccgagc tgcactcgaa gaccctcggc atgaccatcc cggcaggaca
      421 cgcaaccggc atgtcggccg tcgtgacgac ggagaccgaa gaggggatca cgctggagac
      481 ggagtgcatc gggaaggtgt acgcgcccga cgagttcgac cggaacgact ggacgatcgt
      541 cggcgaaccg gacacgcagg tggtcatcaa tcgccccgcg accgtcgagc tgacgtgcgc
      601 gaccatcgtc aaccggctgc ccgacatcgt caacgctccg gccgggtacg tgaccaccga
      661 caagatgccg accaattcct acagagtgaa gccgctgcac acgtacgtcg acgccgaagc
      721 gcttcagtcg tcctcgtcga agcgggccgt gtggcacgac gaatagagcg attccgcgcg
      781 tgcggtgata ccggtttttt caaaaagcat gcggctccga ccaaggcggg gccgcatgct
      841 ttttgagaca ggcgtttccc aagctgattc ttcgcagttt ttcgagcgat gaaagaagcc
      901 gaaggaacga aaagccctgc gatcatcgtg atacaataaa tcaagaaaat tcgccggatc
      961 ggaacttcgc ggcgatctcc cgcgtccggt cgtgttatga gaagttcccc cttgagcggg
     1021 agcaagggac cgaaaaggag cgtgaacctc gatgattgca gtaaaagatg aagtgcgcac
     1081 tcttctggag cggcttcccg acgattgttc ctttgaagat atacagtacc atctctatgt
     1141 tcttgaaaaa gttagaagaa gcgctgcgag agtcgaacag gacggtgcat tggagcatga
     1201 cgaggttgag aagagattgc atcgatggct ctccgtctga aatggtctcc tgacgcggtg
     1261 gaagacctcg agaccatagc ggagtatatc gcgagagact cgtcttcgta cgcctcttct
     1321 gtagtatcca aaattgtaac tgtcgcaaaa actatccccg attttccgcg gagcgggcga
     1381 attgtcccgg aaataggcga tcacgcgata cgcgagcatg tagttttcag ttatcgcctg
     1441 atctatcgcg tcgaacccga tcatatcacg ctgcttgccg tgatccatgg aaagcgattg
     1501 ctggaaaaaa accccgcatg gtgcgacgag tagccccgtt acaccagtgt gcatatggtc
     1561 gaatggcaaa aaagcccgga gtttctggaa cggctatctc ttgatgaatg tcccctcctg
     1621 aagctcgcgg aacgccaggt ccagctcttc gcgagtgttc atcacgatcg ggccgcccca
     1681 cgctatcggc tcgccaagag gtttcgcggc catcagaaag atgcgcgcgc ccgcttctga
     1741 ggcctttgcg cggaacgcgt tccccttctc gaagagcacg gcgtgtttct ccgcgacgcc
     1801 ctctcctgaa gggccgaaaa accctgcgcc ctgaacgata tagatgaaga gcgtcgcttc
     1861 cggatcggtc tccagcagcc actccgcccc cggcgccatc tccgcgtcga ggtagagggc
     1921 gtccgcgtac tctccccgga aggcgccgtc cgtccccttg tagtttccgg agatgatgcg
     1981 gatgttcagc ccctcctccg ccaccgtcgg gacgttctcc ctgacgatgt cgttgtactt
     2041 cggttccgtc atcttgtcct tcgcgggcag attgagccag agctgcgtcc ccagcatccg
     2101 ctcgctcgcc tgcggcatct cctggtggag gatgcccgat ccggccgtca tccactggca
     2161 ctccccgtcg agaatgcgcc ctttgttgcc gaggctgtcg ccgtgcgcga tatcgccccg
     2221 gatcagatag gtgatcgtct cgatcccccg gtgcggatgc caggggaagc ctttgacgta
     2281 gtcctccggg ttcgtcgagt cgaaggcgtc cagcataagg aacgggtcga aatcctgcgt
     2341 gtcgttgtac ccgatgacac gaaccagctt cacccccgcg ccgtccgtcg cgggttttcc
     2401 tgtgacgatt ctgcggatgc ttcgtatttc gttcataact cgttaccctc cttcggctgc
     2461 accgtatgct cattgcgatt atagcatttc attggaagca caccgggaag ccccttgcgg
     2521 ctcgccgacg gagttgtgtg gaaatttacc aaaacatcgt gtatccttac gtgaagggaa
     2581 cgatccgccg aggtgcgtcc ggtcccttcg tggtctttgt ccagttgcaa tactatttcc
     2641 taccctatct gaaggagatg atgccatgcg ctacatcgca ctactcggga gccccaggaa
     2701 agacgggaac accgccgctc tgctcgcctc gtttctcgaa ggcgcggctt ccaaaggaca
     2761 ttcgggggag ctcatccggc tccacgagaa gaagatactc ccgtgcgcgg ggtgcaacgc
     2821 ctgtaaaacg gagggaaagt gcattctctc cgacgacatg acagccctct accccctcgt
     2881 cgaggaggcc gaggtcatcg tccacgcatt cccggtgtac tggtggtcga tgtccgcgca
     2941 gaccaagctc tttcaggaca ggctctacgc gatggactac acgcatttca aggggaaaca
     3001 gctcttcctg ctgacaacgt acggcggcga ggaccccaac tccggtccgc gcctctgcga
     3061 acagtccttc cgcgacatct gcgaattcct ggacatgcag ttccgcgact tcatcggcgt
     3121 ctgctccggg acgacgcccg tccgagacaa caaacgagcc ctcgaaaggg cgtttctcgc
     3181 gggcgcgtcg atctagcgtt ccgctggagg agcttccagt tgcgcccgtt ccacgaaggc
     3241 gatgaaaagg aggaggatac gatgcgcact gatcgcttac gaaccatcct gttgtgtgtc
     3301 tgcgcgcttc ttcttgccgg cgtcgaggag cgcgtcgccg cggcacccgt cgaggcaccc
     3361 gcgctgcagg a
//
LOCUS       DAOTIL010000215         3371 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminobacteriaceae bacterium isolate AMR_MDS_3026
            NODE_33377_length_3371_cov_3.184560, whole genome shotgun sequence.
ACCESSION   DAOTIL010000215 DAOTIL010000000
VERSION     DAOTIL010000215.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868050
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminobacteriaceae bacterium (wastewater metagenome)
  ORGANISM  Aminobacteriaceae bacterium
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae.
REFERENCE   1  (bases 1 to 3371)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3371)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:19:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,224
            CDSs (total)                      :: 3,198
            Genes (coding)                    :: 3,184
            CDSs (with protein)               :: 3,184
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3371
                     /organism="Aminobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_33377_length_3371_cov_3.184560"
                     /isolate="AMR_MDS_3026"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:3034609"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            175..1431
                     /locus_tag="P5201_05610"
     CDS             175..1431
                     /locus_tag="P5201_05610"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HRV98042.1"
                     /translation="MNDHLSEETSSKITRLEEALAKSEDANKKKNAFIANLSHEIRTP
                     LNSIVGVVNLFQGTKLDLEQKKFVEMLSSSSRDLLLLVEELLDLSRLETGQLELKSDP
                     VPLRSCCSQAVRPLAMTAQERRLQMELSIAPDIPEFLCGDSARLRQVLRNIVGTAIAF
                     TAKGKIDIRVSIVHETRETITLQFSVAHSAEGIAGERIKAMLDYSALDRSEFSRAYEG
                     VGLGLIIAKGLAEAMGGGLRAESVGNEHNIFHFTVAMKRTAENKQTSEEPAAQPEQET
                     PDALLALPGRLAILVVDDNKFNRSLTRTILRKKGGPEWNISLAEDGAEALKKISDEHF
                     DLVFMDIQMPGINGLDTTRAIRARERETVAQRLPVIAMTAYAMEGDRQMCLDAGMDDY
                     IAKPVDPDELVEVIIRNLRMITPDER"
     gene            complement(1458..2519)
                     /locus_tag="P5201_05615"
     CDS             complement(1458..2519)
                     /locus_tag="P5201_05615"
                     /inference="COORDINATES: protein motif:HMM:NF018884.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SpoIIE family protein phosphatase"
                     /protein_id="HRV98043.1"
                     /translation="MKNALQTSSPFPLALEVAGFRFNGMNLLDMFGESLVMWETATGH
                     IVYMNEAARVLYDYTLAETAALSIEEIFPSRDEVADRSFSVITTSHRKKSGIFFPVQA
                     TCRTLAQDTEEIMMMVVADISPENLARDDVALASDIQRGFLPGDLVGEPDVEVRSIYR
                     PMFLISGDMFGYHWDPSARVLNGYIYDLMGHGVPAALQTSALMVLFRQAFEDEDRIGS
                     SLTEKLQWVNDTAAERFLSDSFAAAICFRLDLRKKKLSYCAAGINAFLHGVNGELKKV
                     PTPGSLLGLSSRVEFGSGEVSVKPGDYFFFTSDGFLDLLEGTETLRMEFPDAYDHLFQ
                     IGYTGASRDDLSALCVHIR"
     gene            complement(2529..2951)
                     /locus_tag="P5201_05620"
     CDS             complement(2529..2951)
                     /locus_tag="P5201_05620"
                     /inference="COORDINATES: protein motif:HMM:NF024970.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HRV98044.1"
                     /translation="MISIVFSTIEEFAPQRNGLLTLMCSMCGSQRGMRLFVAVNEAVN
                     NALFHANRDLPEACVELVAVDEGASLRISVRSEGDGFEHDLRADPGVFSDFLRESGRG
                     VQIILHVVDEVSIEESGSIVTLRMGKDDSQRRTSSIPS"
     gene            complement(3107..>3371)
                     /locus_tag="P5201_05625"
     CDS             complement(3107..>3371)
                     /locus_tag="P5201_05625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009201440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004478 - methionine adenosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006556 - S-adenosylmethionine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="methionine adenosyltransferase domain-containing
                     protein"
                     /protein_id="HRV98045.1"
                     /translation="AYAIGVAEPVSIMLDTFGTGKLSDEEITRLVRKTFDFRPAAIIR
                     DLDLRKPQYRRLAAYGHIGRIDLDPLPAWERTDRAETLAKALK"
BASE COUNT          807 a          982 c          911 g          671 t
ORIGIN      
        1 gttgtgtact tcgggagcta caacgggaag gtctacgctt tcaggtaacg ccatccggct
       61 cgaaacgaag cgtttcagga caaaggggag cgtcttctcc cctttgtcct tttttctctt
      121 ccggtttata atcactacag aatgaggatc gctcccagag taggaggaac gcccatgaac
      181 gaccatctct ccgaggaaac gtcgtcgaag atcactcgcc ttgaggaagc ccttgcaaaa
      241 tcagaggacg cgaacaagaa aaaaaacgcg ttcatcgcaa atctgagcca tgagatacgg
      301 actcccctca attcgatcgt tggagttgtc aacctgtttc aagggacgaa actcgacctc
      361 gaacaaaaaa aattcgttga aatgctctcg tcatcctcca gggatttact cctgctcgtc
      421 gaggagctgc ttgatctttc gcgcctcgaa acagggcagc ttgaactaaa atcggatccc
      481 gtacctcttc gatcctgctg ctcgcaggcg gtacgccccc tggcaatgac ggcccaagag
      541 cggcgtcttc agatggagct ctcgatagcg cccgatattc ccgaatttct ctgcggagac
      601 agcgcccgac tgcggcaggt actccgaaat atcgtcggta ccgcaatcgc gtttaccgcg
      661 aaaggcaaaa tcgacatcag ggtttccatc gtccacgaaa cccgcgaaac gattactctc
      721 cagttttctg ttgcccactc ggcggaggga atcgccggcg agagaatcaa agcgatgctc
      781 gactattcgg ccctggaccg cagcgaattc tcccgcgcct acgaaggggt gggtcttgga
      841 ctgatcatcg ccaagggatt ggccgaggcc atggggggcg ggctccgggc agagagtgtc
      901 gggaacgagc ataatatttt ccatttcaca gtagcaatga aaaggactgc cgagaacaaa
      961 cagaccagcg aggaaccggc agcacagccg gagcaggaaa ccccggacgc gctcctcgcc
     1021 ctgccgggga ggctcgccat cctcgtcgtc gacgataata agttcaaccg cagcctcacc
     1081 cggacaatcc tgcgaaaaaa gggcggcccg gaatggaaca tttcgctggc ggaagatggc
     1141 gccgaagcgt tgaaaaaaat ttccgacgag catttcgacc tggtcttcat ggacatccag
     1201 atgcccggga taaacggcct ggatacaacc agggccatac gggcacgcga aagggaaact
     1261 gtagcgcagc gtcttccggt catcgccatg accgcctacg ccatggaggg agacagacag
     1321 atgtgccttg acgccgggat ggacgactat atcgcgaagc cggtcgaccc tgacgaactg
     1381 gtggaggtca tcatccgcaa cctccgcatg attactccgg acgagcgata accttgtgag
     1441 ccttgctgtg cgccggctta gcggatatgg acgcagaggg cggaaagatc gtccctgctg
     1501 gctcctgtat agccaatttg gaagagatgg tcgtaggcat ccggaaattc catccgcagc
     1561 gtttccgttc cctccagcag atcaagaaat ccgtcgcttg tgaaaaaaaa gtagtccccc
     1621 ggtttcacag agacctctcc cgatccgaac tctacccggc tggaaagccc gaggagcgac
     1681 cccggagtgg gcaccttttt caactccccg ttcactccgt gcaggaacgc gttgattccc
     1741 gccgcgcagt aggagagttt cttcttgcgc aggtcaaggc ggaaacagat cgccgcggcg
     1801 aacgaatccg aaaggaagcg ctcggcagcc gtatcgttca cccattgcag cttctccgtc
     1861 aggctggagc cgattctgtc ctcgtcctcg aacgcctgcc ggaagaggac catcagggcc
     1921 gacgtctgga gcgcagcggg aacgccgtgt cccatgaggt cgtagatgta gccgttcaaa
     1981 acccgtgcgg aaggatccca atgatagcca aacatatccc cgctgatgag aaacatcggc
     2041 cggtagatgc tccgcacttc gacatcgggc tctcccacaa gatctccggg aagaaatccc
     2101 cgctgaatgt ccgaggcgag cgcaacgtcg tcacgcgcca gattttccgg cgagatgtcg
     2161 gcgacaacca tcatcatgat ttcttcggtg tcctgcgcca acgtgcggca cgttgcctgc
     2221 acgggaaaga agatgccgct cttctttctg tgcgaggtcg taatcacgga gaacgacctg
     2281 tcggcaactt catcgcggga aggaaaaatt tcctcgatgg aaagggccgc ggtctccgcc
     2341 agcgtgtagt cgtagagcac cctcgccgcc tcgttcatgt acacgatatg ccccgtcgcc
     2401 gtctcccaca tcacgagaga ttctccgaac atatccaaca gattcattcc gttaaaacga
     2461 aaaccggcaa cttccagagc caaggggaag ggcgaagatg tctggagagc gtttttcatg
     2521 cgatcacctc atgacggaat cgaggaggtt ctcctctgcg aatcatcttt tcccatacga
     2581 agcgtgacga tacttcccga ctcttcaatg ctcacctcgt caacgacgtg gagaataatt
     2641 tgcactcccc ggcccgactc cctgagaaaa tcggaaaaga cgccgggatc agcacgcaag
     2701 tcgtgttcaa aaccgtcgcc ttcgctccgt acggatatac gtaagctcgc gccctcatcg
     2761 acggcaacaa gctccacgca cgcttcagga aggtccctgt ttgcgtgaaa aagcgcattg
     2821 ttgaccgcct cgtttaccgc tacaaaaaga cgcatgcctc tctgcgaacc gcacattgag
     2881 cacataagcg ttaaaagacc gtttctttga ggggcgaatt cctctattgt ggagaataca
     2941 atcgatatca agttctcctc tctgcacata ccatcactcc tcgcctccgg aaatagggga
     3001 cgccgttcgt cttcttacgg ccataatagc acagagaaag agcgaggaaa agaaaaagga
     3061 ggccgcatat cgcagcctcc cgctctggag aaaacctaaa tcggcgctat ttcagcgctt
     3121 tggccagagt ttcggcccgg tccgtccgct cccatgcggg cagcggatca aggtcgatac
     3181 ggccgatgtg tccgtaggcc gcaaggcggc ggtactgggg tttgcgaaga tcgaggtcgc
     3241 gaatgatcgc cgccgggcgg aagtcgaacg tcttgcgcac gaggcgtgtg atctcctcgt
     3301 cagagagctt tcccgtcccg aaggtatcca gcattatgga aacaggctcc gctacgccaa
     3361 tggcgtatgc c
//
LOCUS       DAOTIL010000216         3369 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminobacteriaceae bacterium isolate AMR_MDS_3026
            NODE_33397_length_3369_cov_3.372661, whole genome shotgun sequence.
ACCESSION   DAOTIL010000216 DAOTIL010000000
VERSION     DAOTIL010000216.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868050
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminobacteriaceae bacterium (wastewater metagenome)
  ORGANISM  Aminobacteriaceae bacterium
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae.
REFERENCE   1  (bases 1 to 3369)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3369)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:19:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,224
            CDSs (total)                      :: 3,198
            Genes (coding)                    :: 3,184
            CDSs (with protein)               :: 3,184
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3369
                     /organism="Aminobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_33397_length_3369_cov_3.372661"
                     /isolate="AMR_MDS_3026"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:3034609"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..241)
                     /locus_tag="P5201_05630"
     CDS             complement(<1..241)
                     /locus_tag="P5201_05630"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRV98046.1"
                     /translation="MMTSRAERSLCVIIARSRGFRETKHRTTRLFNVFRAALCFIWPC
                     GDEPEICRKKRGKNTMGLKRGTVRVAVVQAGSVIMD"
     gene            402..1181
                     /locus_tag="P5201_05635"
     CDS             402..1181
                     /locus_tag="P5201_05635"
                     /inference="COORDINATES: protein motif:HMM:NF012707.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HRV98047.1"
                     /translation="MGRFFTAFLLLVLLGSTACVSVASAGETVVRITNGDWPPFLGEH
                     LPGNGAISQVVRESFALKGIETRYGFFPWARALALAAEGKWDASVGWAFSEERARDFF
                     FSDALVDLPVLLFSLNEKKVEWKEWQDLAGLLIGVTRGYHYGNEFEKASEEGLFRTDP
                     SPDDETSLRKLLGKRVDAVAVDRYVGIELINRGFALEADRFWYCETPVRSAPYSLLFS
                     KKAPNAEELLAAFNEGLAELKASGRYQEILDDAASGKYSTP"
     gene            complement(1504..2253)
                     /locus_tag="P5201_05640"
     CDS             complement(1504..2253)
                     /locus_tag="P5201_05640"
                     /inference="COORDINATES: protein motif:HMM:NF024950.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HRV98048.1"
                     /translation="MEFAGRTALVTGAGTGNGEAIAERLFTGGASVGLVSRRLESVDA
                     VCRRIDPEGERTLAIEADVRDPKAMEAAVQRIVERFGSLDLAVNNAGITGPAGAPVQD
                     IESETWLEVIATDLTGVFYSMKYEIPAMLANGSGAIVNMSSANGLVGLPGMAAYTAAK
                     HGIIGLTRSAALELAESKIRVCAVAPGYVATPRILDSGKEVTDWMAGVHPMKRLAARE
                     EVADLVAFLLSDSASFITGSVHSIDGGYTAQ"
     gene            2573..>3369
                     /locus_tag="P5201_05645"
     CDS             2573..>3369
                     /locus_tag="P5201_05645"
                     /inference="COORDINATES: protein motif:HMM:NF013961.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglutaminase-like domain-containing protein"
                     /protein_id="HRV98049.1"
                     /translation="MNEFSFFATQGPLSRPGAHAELFDSLPADIGELCAVVQGVTVHP
                     FWAERYGVTLAAERGAEVQLRTMEKRLARTLELDSRPLTVPRPLELKTVGNCRDHTLL
                     LVSMLRSQGVPARARCGFGTYFLPGHFEDHWVAEYWNESEKRWILVDAQLDAFQRGVL
                     NVSFDPLDVPRDRFIGGGAAWRMCREGVADPNAFGIFDMRGMGFVGSNLVRDVAALNK
                     VELLPWDCWGMMLERDTDDSDLALLDRLAELTEGDVPDFGAVCELYES"
BASE COUNT          642 a         1010 c          970 g          747 t
ORIGIN      
        1 tgtccatgat cacggacccc gcctgcacca ccgctacccg taccgttccg cgcttcagac
       61 ccattgtgtt cttcccccgt ttcttccggc aaatttccgg ttcgtctccg cacggccaaa
      121 tgaagcaaag cgccgcccta aacacattga aaagacgcgt cgtccggtgt ttcgtttcgc
      181 gaaaaccccg cgatcttgct attattacac aaagagaacg ctccgctcga ctggtcatca
      241 tgtcttaaat ttcttccgaa aaaaacgagc ccgctgaagg ggtgcttgac acccataaca
      301 tttcggccta caatttcaag aaatcaatac aaattgtatt ttttagtgct attttccatt
      361 tgcgaatttc gcattatacc atcacgcggg aggtgttgct catggggcga tttttcacag
      421 cgtttcttct tctcgttctt cttggttcga ctgcctgcgt ctccgttgct tcggccggag
      481 aaacggtcgt ccgtattacg aacggcgact ggcctccgtt tctgggggag catcttcccg
      541 gaaacggagc gatctcgcag gttgtgaggg aatccttcgc ccttaagggc atcgaaacga
      601 ggtacggatt cttcccgtgg gcgcgcgctc ttgccctcgc cgcggagggg aagtgggatg
      661 cgtccgtcgg gtgggctttt tcggaagaac gagcgcggga ctttttcttc agcgacgcgc
      721 ttgtcgatct gccggtgctt ctcttctccc tgaacgaaaa gaaggtcgag tggaaggagt
      781 ggcaggacct tgcagggctc ttgatcggag ttacgcgggg gtatcactac gggaacgagt
      841 tcgaaaaagc gagtgaagaa ggtctcttcc gaaccgatcc atctcctgac gatgagacga
      901 gcctcaggaa gctgctcgga aaaagagtgg atgccgtcgc agtcgacaga tatgtaggta
      961 tcgagctgat caaccgcgga tttgccctgg aagccgaccg gttctggtac tgcgaaaccc
     1021 ccgtccgctc ggcgccgtac tctctgcttt tttcgaaaaa agcgccgaac gccgaggaat
     1081 tgctggcagc attcaatgag gggctcgccg agctgaaggc aagcggccga taccaggaga
     1141 tactggacga cgcggcgtcc ggcaagtatt cgactccgtg acgttttcgc ccgtacgagc
     1201 cgtggagacg acggaacgac tctacaatca tcggtttggc attttggaac cgttgcgtta
     1261 cgttcttcac cggttactcg tgaagccacg gggcgtagct caagccgatc aacacccctt
     1321 cggggctgat gaaccgcgcg acggtctgcc cccacggttc cgtccgcgcc tcgtgaacga
     1381 attcacgccg ccgggtcttt cgtaatgacg gcgaaacccg ctacgctctc gatttttatc
     1441 ggtccgttca ttgtatttcc tccttttgta ctcatagagt cgaacgaggt gtatgtgatc
     1501 acattattgc gcggtgtacc cgccgtcgat tgaatgaacg ctcccggtta tgaaggacgc
     1561 gctatcgctt aacaagaagg cgaccaaatc agcgacctct tcccttgcgg cgaggcgctt
     1621 catggggtgc acccccgcca tccagtccgt gacttctttg ccggaatcca gtatccgcgg
     1681 ggttgcgacg taacccggcg cgacggcgca tacgcgtatc ttggattcgg ccaattcgag
     1741 cgccgcggag cgggtgagcc cgatgattcc gtgtttggcc gctgtgtacg cagccattcc
     1801 cggcaagccc accaagccat tggcggacga catgttgacg atcgcgccgg acccgtttgc
     1861 gagcatcgcg ggtatctcat acttcattga gtagaagacg cccgtcagat cggttgcgat
     1921 cacttccaac cacgtttccg attctatgtc ctgaaccggc gccccggccg gaccggtgat
     1981 ccccgcgttg ttcacggcca gatcaagcga gccgaagcgt tcgacgatgc gttgcaccgc
     2041 agcctccatc gccttgggat cgcgcacatc ggcctcgata gccagcgtcc gttcaccttc
     2101 agggtcaatc cgccggcaga ctgcgtccac tgattcaaga cggcgactca ccagcccgac
     2161 cgacgctccg ccggtgaaga gccgctcggc tattgcttcg ccgttccctg ttccggcgcc
     2221 ggtaaccagc gctgttcttc ctgcaaattc catatcgcac ctccaggctg cgtcaggtgt
     2281 ttcctcattg ttgcacgatg gttatgccat gactcccaat cattctgctt cttgacaata
     2341 aaacttcttt agaaaatagt accacgaatg gcgccttctc ggagacctca ccctccgttt
     2401 ttgaaagagg gccgggtggt gtctgcaccg aggcgccggg ccgaggcaac tgatacaata
     2461 cataccgtcg gacgggagtc gatccccgtt cccgaatact gttagacgcg atccgccgga
     2521 cgctgcggcg ttcgaagcgg ggtacgccgc tctctgttta aggaggaaac ctatgaacga
     2581 attctcgttt ttcgcgactc aagggcctct cagcaggccc ggagcgcacg ctgaactttt
     2641 cgattctctc cctgcggaca tcggggagtt gtgtgcggtc gtccagggcg tcaccgttca
     2701 ccccttctgg gccgaacggt acggggtgac gctcgccgcc gagaggggcg ccgaggtgca
     2761 gctccgtacc atggagaaac gtctggcccg cactctggaa ctcgattcgc gcccactgac
     2821 cgttcctcgg ccgctggaat tgaagacggt cggcaactgc cgcgaccaca cgctgctgct
     2881 ggtgtccatg ctgcgcagtc aaggggtccc ggcccgcgcg cgctgcggat tcgggacgta
     2941 cttcctgccc ggacacttcg aggatcactg ggtcgccgaa tactggaacg aatccgagaa
     3001 acgctggata ctggtcgacg cgcagctcga cgctttccag cgcggcgtgc tgaacgtttc
     3061 attcgatccg ctggacgtgc cccgggaccg cttcatcggc ggcggcgcgg cctggaggat
     3121 gtgccgcgag ggggttgcgg acccgaacgc cttcggcatc ttcgacatgc ggggaatggg
     3181 tttcgtggga agcaatctcg tccgcgacgt cgcggcgctc aacaaggtcg aactgctgcc
     3241 gtgggactgt tgggggatga tgctcgaacg cgacacggac gactccgacc tggcgctgct
     3301 cgatcgtctg gccgagctga ccgagggcga tgttccggac ttcggggctg tatgcgaact
     3361 ctacgagtc
//
LOCUS       DAOTIL010000217         3345 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminobacteriaceae bacterium isolate AMR_MDS_3026
            NODE_33720_length_3345_cov_3.869605, whole genome shotgun sequence.
ACCESSION   DAOTIL010000217 DAOTIL010000000
VERSION     DAOTIL010000217.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868050
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminobacteriaceae bacterium (wastewater metagenome)
  ORGANISM  Aminobacteriaceae bacterium
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae.
REFERENCE   1  (bases 1 to 3345)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3345)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:19:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,224
            CDSs (total)                      :: 3,198
            Genes (coding)                    :: 3,184
            CDSs (with protein)               :: 3,184
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3345
                     /organism="Aminobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_33720_length_3345_cov_3.869605"
                     /isolate="AMR_MDS_3026"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:3034609"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..702
                     /locus_tag="P5201_05650"
     CDS             <1..702
                     /locus_tag="P5201_05650"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HRV98050.1"
                     /translation="PLNILIAEPDMMLRTLYRKTVSEVPGYVFAGYADNGDALHSIVA
                     CTAVNVVLLDILLPRFGSLEGFRAMRAEFPRTDFIILSSEKTPDVVRGTICSGAFDYL
                     IKPFEWERFERALAAYSEYHRSLVDRKRPWRQEDIDRLPGFRKQLPENSQPTPKGFQE
                     TLLNRLFNLLRESSSPLSASEAGRMLGISRSSARRYLEYLADEGEIAVQYEFTQVGRP
                     RKLYSVHFRAKEGSV"
     gene            788..997
                     /locus_tag="P5201_05655"
     CDS             788..997
                     /locus_tag="P5201_05655"
                     /inference="COORDINATES: protein motif:HMM:TIGR00638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TOBE domain-containing protein"
                     /protein_id="HRV98051.1"
                     /translation="MKISARNQIRGKVTAIKEGAVEAQVTLDIGGGQTMVSVITMDSV
                     ANLGLKVGSEAVAIVKADSVMLAVN"
     gene            complement(1078..1908)
                     /locus_tag="P5201_05660"
     CDS             complement(1078..1908)
                     /locus_tag="P5201_05660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008710078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HRV98052.1"
                     /translation="MNLRKVCAFVLIAAFLFTGAGTGTAAEVLRMATTTSTDNTGLLD
                     YLAPLLLNEKGIEIRWVSVGTGKALEYGRNGDVDVVLVHDPEAEAKFVAEGSGVNRRQ
                     VMFNDFIIVGPASDPAKVQGLSVTAALKKIAQDGAPFASRADKSGTHMAELRLWAAAG
                     MPEPDKEKWYIQTGQGMLETLMIAGERKGYAFTDRGTFIKYEDSRKEQADLAIIVEGD
                     ASLKNQYSVMAVNPAKWENIRYDIALQYIDWLISPSTQKAIADFQLLGKQLFFPNAAR
                     "
     regulatory      complement(1986..2105)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01055"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="molybdenum cofactor riboswitch; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /bound_moiety="molybdenum cofactor"
                     /db_xref="RFAM:RF01055"
     regulatory      2248..2367
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01055"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="molybdenum cofactor riboswitch; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /bound_moiety="molybdenum cofactor"
                     /db_xref="RFAM:RF01055"
     gene            2421..3215
                     /locus_tag="P5201_05665"
     CDS             2421..3215
                     /locus_tag="P5201_05665"
                     /inference="COORDINATES: protein motif:HMM:NF012738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HRV98053.1"
                     /translation="MPRSLRERLFMLSMGSFLLLYILWPLGGLFLRADWSLVASASHD
                     GEVLSALWRSIWTAAAATGVIALFGTPLAYFLARQSFRGKSILEAVIDVPIMIPHTVA
                     GIAVLMAFSPKAPAGALLTSMGFSPVNSNLGIILACMFVSIPFYVDAARDAFYSVSPR
                     TEKVARTLGASFPHVFFRISLPLARKGLISGLIMSWARGVSEFGAIVIIAYHPMVAPT
                     LIYDRFATFGLKYSTPVAVQLILVCLGMFVVLRILAAGKRKEDAEI"
BASE COUNT          633 a         1018 c          962 g          732 t
ORIGIN      
        1 ccgctgaaca ttctcatcgc cgagccggac atgatgcttc gcacgctgta cagaaagacc
       61 gtctcggaag ttcccggcta tgtcttcgcc ggctacgcgg acaacggcga cgctttgcat
      121 tccatcgtcg cctgcaccgc cgtcaacgtg gttctcttgg acattcttct gccccgcttc
      181 ggcagcctgg agggattccg tgcgatgcgc gcggaatttc caaggacgga cttcatcatc
      241 ctctcctcgg agaaaacgcc cgacgtcgtc aggggaacga tctgctcggg cgcgttcgac
      301 tatctgatca aaccgttcga gtgggagcga tttgaacgag cgcttgccgc atacagcgag
      361 taccaccgca gccttgtcga cagaaaacgc ccttggagac aagaggatat cgacaggctt
      421 cccggtttcc ggaagcagct tcccgaaaat tcccaaccga cgcccaaagg ttttcaggaa
      481 acccttctga atcgactctt caatcttttg cgggagtcgt ccagtcccct ctccgcttcg
      541 gaagcggggc gaatgctcgg catctccagg tcgagcgccc ggcgctacct tgagtacctc
      601 gccgatgagg gggagatcgc ggtacagtac gagttcacac aggtaggccg cccgagaaag
      661 ctctactcgg ttcactttcg ggcaaaagaa ggatctgtct gaactctttt cagctgcaca
      721 aacttgcaaa agatgtattt aggtgcgata ataccggaag aatctagaac agaagggagt
      781 gcatatcatg aaaataagcg cacgcaatca gatccgcggc aaggtgaccg ccatcaaaga
      841 aggcgccgtt gaggcgcagg taacgcttga catcggcggc ggccagacga tggtctccgt
      901 gatcacgatg gactccgtgg ccaacctcgg cctcaaggtg ggatccgaag ctgtcgccat
      961 cgtcaaggcc gacagcgtca tgctcgcggt caattagtaa ttccgcaaga atcagacacc
     1021 aaaaaagccc cctgtcgggg gcttttttta ttttgtaaaa cgtactgagg ccgtcgttca
     1081 gcgggcggcg ttggggaaga agagctgctt cccgaggagc tggaagtcgg cgatcgcctt
     1141 ctgcgtcgac ggggagatca gccagtcgat gtactgcagc gcgatgtcgt agcggatatt
     1201 ctcccacttc gcggggttca ccgccatgac gctgtactgg ttcttcaagg aggcgtcgcc
     1261 ctcgacgatg attgcaaggt cggcctgctc cttgcgcgag tcctcgtact tgatgaaggt
     1321 gccgcggtcg gtgaaggcgt accccttacg ctcgccggcg atcatcaagg tttcgagcat
     1381 tccctgcccg gtctggatgt accacttctc cttgtcgggc tcgggcattc cggcggcggc
     1441 ccaaagacga agctcggcca tgtgcgtgcc ggacttgtcc gcgcggctcg cgaagggcgc
     1501 gccgtcctgc gcgatcttct tcagcgccgc cgtcaccgag agtccctgca ccttcgccgg
     1561 gtcggaagcc gggccgacga tgatgaagtc gttgaacatc acctgtcggc ggttcactcc
     1621 cgaaccttcg gcgacgaact tcgcctcggc ctcgggatcg tggacgagca ccacgtcgac
     1681 gtcgccgttc cggccgtact caagcgcctt ccccgtgccg acggagaccc agcggatctc
     1741 gatgcccttt tcgttcagaa gcagaggggc gaggtaatca agcagtcccg tgttgtcggt
     1801 gctggtggtg gtggccatgc ggagcacctc cgccgccgtt cccgtaccgg ctccggtaaa
     1861 aagaaatgcc gcgatcaata caaaagcaca taccttcctc aaattcattt tcattcctcc
     1921 tcgatatgat tttgtcccgc atacaaaaag ccgcctcccc catatacggc gggagacggc
     1981 tgcatcagca tccgaatcca tctccttgcc gaacacggga ggctcgcctg tttccggacg
     2041 aaccctgacg gcccgccgcc gtgggaattt cctgtaggca aacgggctcg gagaacaagt
     2101 tgtcttttat ttgatgaata tcataccata aaacctcgag tcgaggtatc cgccgacagg
     2161 catgagaacg agcatttttc gcctttactt gataaagagt atcattgtca ttttctgaac
     2221 tacgtggtat tcttgcttcg tccgggtttt tcggacatcc tccgagccgc actccctacg
     2281 gaatacgttc catgggagac ggccaacggg ttctcccgga aacgggaggg cctcccgcgt
     2341 tcggaaagga ggagcggccg gtgtatccca gggctgtctc ccatggagac ggcccttttt
     2401 atgtgtctgg agggtggaat atgcctcgtt cgcttcggga gcgtttgttc atgctgtcca
     2461 tgggttcctt cctgctgctg tatattctct ggccgctcgg cggactcttt ctgcgcgccg
     2521 actggagtct cgtcgcctca gcgtcgcacg acggggaggt gctctccgca ctgtggcgca
     2581 gcatctggac ggctgcggcg gcgacgggag tcatcgcgct cttcgggacg cctcttgcct
     2641 acttcctggc gcgccagtcc tttcgcggaa agtcgatcct cgaagcggtc atcgacgtgc
     2701 ccatcatgat tcctcacact gtggcaggca tcgcggtact catggccttc tcgccgaagg
     2761 ctccggcggg ggcgctgctc acctccatgg gattctctcc tgtgaacagc aatctcggaa
     2821 tcatcctcgc atgcatgttc gtctccattc cgttctatgt ggacgcggcg cgcgacgcct
     2881 tctactcggt ctccccgcgg acggagaaag tggcccggac gctcggggcc tcattcccgc
     2941 acgttttctt ccgcatctcc ctccccctgg cgaggaaagg attgatctcc ggcctcatca
     3001 tgagctgggc gcggggagtg agcgaattcg gggcgatcgt catcatcgcc taccacccga
     3061 tggtggcccc cacgctgatc tacgaccggt tcgccacctt cggactgaag tactccacgc
     3121 ctgtggccgt ccagctcatc ctcgtctgcc tcggcatgtt cgtcgttctg cggatactgg
     3181 ccgcagggaa gcggaaggag gatgccgaga tatgatcgag cttgaaaatc tctcggtcaa
     3241 tctccgtacc ttccggctgg aatcgctctc cctccggata gctccgggcg agttcttcat
     3301 gatcgtgggg ccgagcgggg cggggaagac gatcctgctg gaatc
//
LOCUS       DAOTIL010000218         3336 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminobacteriaceae bacterium isolate AMR_MDS_3026
            NODE_33815_length_3336_cov_4.133496, whole genome shotgun sequence.
ACCESSION   DAOTIL010000218 DAOTIL010000000
VERSION     DAOTIL010000218.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868050
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminobacteriaceae bacterium (wastewater metagenome)
  ORGANISM  Aminobacteriaceae bacterium
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae.
REFERENCE   1  (bases 1 to 3336)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3336)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:19:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,224
            CDSs (total)                      :: 3,198
            Genes (coding)                    :: 3,184
            CDSs (with protein)               :: 3,184
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3336
                     /organism="Aminobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_33815_length_3336_cov_4.133496"
                     /isolate="AMR_MDS_3026"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:3034609"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(71..1081)
                     /locus_tag="P5201_05670"
     CDS             complement(71..1081)
                     /locus_tag="P5201_05670"
                     /EC_number="3.1.3.7"
                     /inference="COORDINATES: protein motif:HMM:NF013528.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004527 - exonuclease activity [Evidence
                     IEA];
                     GO_function: GO:0008441 - 3'(2'),5'-bisphosphate
                     nucleotidase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional oligoribonuclease/PAP phosphatase
                     NrnA"
                     /protein_id="HRV98054.1"
                     /translation="MNDSYASSDGFPALIRILSEASEWIVLSHVKPDGDALGSASAFA
                     SVGRKLGKTVVWGGPDEYPSRYLFLEGSGEFRPNLKLSSLALTEGSVVLALDTSTRAR
                     SVEDLDALPENVPLLNVDHHADNELFGTVNYVDPSSSSTGEIAWLLLREWGIPLEIDV
                     LEAVYTALATDCGNFAFSCTTPRTHRVAAELLDGGVSPAKLDERIRCNASIPGLRLRA
                     VALSRAFVAGGFAAFTWLRNDDFLHNGSERSATEFLVNDLLTLKDVSFAAFFVEDEEC
                     VRVSLRSRGVVLAAEIARVFGGGGHPQAAGCKLPLPLSDAVESVRSLVEERYAERIAP
                     AE"
     gene            complement(1098..1511)
                     /gene="rbfA"
                     /locus_tag="P5201_05675"
     CDS             complement(1098..1511)
                     /gene="rbfA"
                     /locus_tag="P5201_05675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006300998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosome-binding factor RbfA"
                     /protein_id="HRV98055.1"
                     /translation="MTTFRMQRINKQIQREVSLLLELRIKNETAKKAIITDVDCSRDL
                     EHAKVYFTTVDRSEREEVGKALGTVAGALRSQLGKLLTIRQIPALSFFFDTSEEYGRS
                     IDVLLDSLKTEDSVIERDEDDETEDASDTDELPEA"
     gene            complement(1824..>3336)
                     /gene="infB"
                     /locus_tag="P5201_05680"
     CDS             complement(1824..>3336)
                     /gene="infB"
                     /locus_tag="P5201_05680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008709793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="translation initiation factor IF-2"
                     /protein_id="HRV98056.1"
                     /translation="EARSPIVTVMGHVDHGKTTLLDFIRKTNITAREAGGITQHIGAS
                     IVMHEGRRIVFLDTPGHEAFTSMRARGAQATDVAVLVVAADDGVMPQTREAINHAKAA
                     GVPIVVAINKVDKPAAKADRVRQQLSDVGLAPEEWGGSTIMVEVSAKSGQGIPQLLEM
                     ILLVSEMEDLKADPTVRPEGVVVEAKLDKGKGPVATVLVQQGSLCKGDIVLFDTAWGK
                     IRAMIDAEGNPIDCAGPSTPVEILGLTAVPQPGEVFKAVENERIARDALSVKEQEVRN
                     AQQSGPRKLTLEELYSQLKEGDIPQLNLLLKCDVQGSSEALAASLEKLATNEVGINIV
                     HRGVGRIAESDVMLASASNAVIIGFNVRPDANAKKVAESEGVQVRLYDIIYDAIDDVK
                     AALEGMLKPTIRENTLGQAEIRQVIRVPKAGNVAGSYVLEGVIRRNAKARLVRNGIVL
                     WDGSLANLRRFKDDAREVAAGYECGISLSNFQDFEEGDIVEAYEIIEEKRHLS"
BASE COUNT          712 a         1029 c          867 g          728 t
ORIGIN      
        1 tatcttgaac tctctgttca ggcgcctttt cgcgatggct acacagacgg cgcttcgtac
       61 gccggcaggt ttattcagca ggagcaatcc gctcggcata ccgttcctcc accagagagc
      121 gtacactctc cacagcgtcc gataatggaa gaggaagctt gcaccccgcg gcctggggat
      181 gccctcctcc accgaatacg cgcgctattt cagccgccag aacgacgcct ctcgaccgga
      241 gcgatacgcg gacgcactcc tcgtcctcca caaagaaagc ggcaaacgaa acatccttga
      301 gcgtgaggag atcgtttacc aggaactcag ttgcagagcg ttcgctgccg ttatggagaa
      361 aatcatcgtt cctcaaccat gtaaacgccg caaaaccgcc tgcaacgaag gctctggaca
      421 atgccaccgc ccttaaacga agaccgggaa tggatgcatt gcaccggatt cgctcatcca
      481 actttgccgg agaaacccct ccgtcaagaa gttccgctgc aaccctgtgg gtccgcggcg
      541 tagtacacga aaacgcaaag ttcccgcagt cagtcgcgag agcagtgtac acagcttcca
      601 gcacatcgat ttccaagggt atcccccact cgcgaaggag cagccaggct atctcgccgg
      661 tcgaagagga ggagggatcc acatagttga cagtcccgaa caattcattg tccgcatggt
      721 gatccacgtt gagcaggggc acgttttcgg gaagagcgtc aagatcctcc acagaacgcg
      781 cacgggtcga cgtatccaac gcgagaacca cgcttccctc cgtcagcgcc agcgaggaaa
      841 gcttcaggtt cggacggaac tccccggaac cttcaagaaa caggtaacgg gaagggtact
      901 cgtccgggcc gccccagacg acagtctttc cgagtttccg cccaaccgaa gcgaaagcgc
      961 tcgcggaacc gagcgcatcg ccgtcgggtt taacatgtga gagaacaatc cattccgagg
     1021 cttcagaaag aatgcgaatg agcgcgggga agccgtccga cgaagcatac gaatcgttca
     1081 tttcatcctc cccgtcacta cgcctccggc agttcatccg tgtcggaggc gtcttcggtc
     1141 tcgtcgtctt catccctctc aataacggaa tcctcggtct tcaacgaatc cagcagcaca
     1201 tcgatactgc gcccgtactc ctcgctggta tcgaaaaaaa aggaaagggc gggaatctgc
     1261 cgtatcgtca aaagttttcc gagctgactc cgcagagccc ctgcgaccgt tccgagcgcc
     1321 ttccctactt cttcccgctc ggaacggtcg actgtcgtaa agtacacttt tgcatgttca
     1381 agatcacgag aacagtcgac atcagtgatg atcgctttct ttgccgtttc gttctttatg
     1441 cgcagctcca gcagaagcga aacttctctc tggatctgct tgttgattct ctgcattcga
     1501 aatgtcgtca taacgtgtta cctcgcgcca gtgatatagg atcgagaact ccccggtctc
     1561 ctcctcgttg gttaaaaagg agtacaccgc ctggagccgc tcctccgcca tagcaacccc
     1621 tgaagcggct gcggagacag ccacaaaggc ctcggaccag cttccgtcgg gcccgagatc
     1681 catggctgaa acattccaac gtcgtctgat cctgtcgagc agggattgcg tcacctgtct
     1741 ccggtccttc atgcttctgc ttcccattat ttcgagggca acaaacagga aacctgagta
     1801 cggaacggga acccttcggt tcattaggag agatgccttt tctcctcgat gatctcgtat
     1861 gcttccacga tgtcgccctc ttcgaagtcc tggaagttgg agaggctgat gccgcattcg
     1921 taccctgcgg cgacctcgcg tgcatcgtcc ttgaaacggc gaaggtttgc aagggagccg
     1981 tcccagagca caataccatt gcgtacgagg cgggctttcg cgttccttcg aattacgcct
     2041 tcaagcacgt agcttccggc gacgttaccg gccttcggga cgcgaatgac ttggcggatc
     2101 tccgcctggc ccagcgtgtt ctcccgaatg gtaggcttca gcataccttc gagggcggcc
     2161 ttcacgtcgt cgatagcgtc atagatgatg tcgtacaaac gtacttggac accttccgac
     2221 tcggctactt tcttcgcatt cgcgtccggg cgcacgttaa aaccaataat aacggcgttc
     2281 gacgcggagg cgagcatcac gtcggattcg gcgattctgc cgactccgcg gtgaactatg
     2341 ttaatcccga cttcgttcgt cgcaagtttt tccaaagatg cggcaagcgc ctcgctcgac
     2401 ccctggacat cgcacttcag cagaagattg agctgcggaa tatcgccttc cttcagctgc
     2461 gagtagagct cctccaacgt gagttttctc ggaccgctct gctgggcatt acgaacttcc
     2521 tgctctttta cgctgagagc gtcgcgggct atgcgttcgt tttcaaccgc tttgaagact
     2581 tcacccggct gcggtacggc ggtaaggccg agaatttcca ccggcgtgct cgggcccgca
     2641 cagtcgatcg ggttcccttc agcatcgatc atagccctga tcttgcccca cgcggtatcg
     2701 aacaacacga tatctccctt gcagagagag ccttgctgca caagaaccgt tgcaacgggt
     2761 cctttcccct tgtcgagctt cgcttccacg acaacgcctt cggggcgaac cgtcggatcc
     2821 gccttcagat cttccatttc cgagacaaga agaatcatct ccagaagctg cggaataccc
     2881 tgtcccgatt tcgcggaaac ttcaaccatg atcgtggagc cgccccattc ctcgggcgcc
     2941 agaccaacat cggaaagctg ctgccgaact ctgtccgctt tagctgcagg cttgtccact
     3001 ttgtttattg ccacaacgat gggcaccccg gcggccttgg catggtttat cgcctcgcgc
     3061 gtctgcggca ttactccgtc gtccgctgct acgacgagaa ctgcaacgtc ggtagcctgg
     3121 gctccccgtg cgcgcatcga ggtgaacgcc tcgtgtccgg gagtatccag aaaaacgatt
     3181 cgtcttcctt cgtgcatcac gatggaagcg ccgatgtgct gcgttatgcc gcccgcctcc
     3241 cgggcggtga tgttcgtctt gcggatgaag tcgagaaggg tcgttttccc gtggtcgaca
     3301 tgccccatca ccgtaacgat cggagaacgg gcttca
//
LOCUS       DAOTIL010000219         3324 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminobacteriaceae bacterium isolate AMR_MDS_3026
            NODE_33964_length_3324_cov_3.260018, whole genome shotgun sequence.
ACCESSION   DAOTIL010000219 DAOTIL010000000
VERSION     DAOTIL010000219.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868050
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminobacteriaceae bacterium (wastewater metagenome)
  ORGANISM  Aminobacteriaceae bacterium
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae.
REFERENCE   1  (bases 1 to 3324)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3324)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:19:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,224
            CDSs (total)                      :: 3,198
            Genes (coding)                    :: 3,184
            CDSs (with protein)               :: 3,184
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3324
                     /organism="Aminobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_33964_length_3324_cov_3.260018"
                     /isolate="AMR_MDS_3026"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:3034609"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..448
                     /locus_tag="P5201_05685"
     CDS             <1..448
                     /locus_tag="P5201_05685"
                     /inference="COORDINATES: protein motif:HMM:NF013539.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HRV98057.1"
                     /translation="LKRPLPDDAKRMLTLLWGDEGRTIGEALQGALPAISLGSHGALM
                     TAFANDADGRFAFAQQAMGYARPGDVALGISTSGNAENVRFALMAAKSRGASTVALTG
                     ADGGLLAKYADCCIRVPDRRTFRVQEHHLRIYHFLCAFVESELFDE"
     gene            494..1468
                     /locus_tag="P5201_05690"
     CDS             494..1468
                     /locus_tag="P5201_05690"
                     /inference="COORDINATES: protein motif:HMM:NF013408.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I mannose-6-phosphate isomerase"
                     /protein_id="HRV98058.1"
                     /translation="MKPFSLVPVFKDYLWGGTKLKTRFNKRTEVSPLAESWELAAHPD
                     GDCRIANGSFAGTPLSEFLRKFPESLGSGRACGGGLPVLIKLIDAREPLSVQVHPDDV
                     YAMRTEGGAGKTEMWYVLDREPGAFLYCGFQRDVSREELTRRIADGTVTEVLHKAPVE
                     PGDVFFMEAGTVHAIGAGILIAEIQQSSNTTYRVFDYGRRGPDGKERPLHIEKALDVA
                     RRGPACSVPPGSRPPVILPGSTLRRLARCAHFSVELLELSEHCEYRTDETSFLSLLCL
                     EGSARLAEGEWGFDIAKGDSVFVPARKGSVFLEGRGTFLLTTVPDGEL"
     gene            1606..3135
                     /gene="guaA"
                     /locus_tag="P5201_05695"
     CDS             1606..3135
                     /gene="guaA"
                     /locus_tag="P5201_05695"
                     /EC_number="6.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008522138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003922 - GMP synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016462 - pyrophosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006177 - GMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing GMP synthase"
                     /protein_id="HRV98059.1"
                     /translation="MNNIVILDCGSQFTQLIARRIRELKVHSEILPWDAPIERIKSAA
                     LSGVVISGGPMSVSDEGAPTVPEELFQLGVPVLGVCYGMQLATKLFGGVVTSGSSREY
                     GRTAISVKEESALFSGTAASFEVWMSHGDHIDRLAPGFRCVAETANGVPAAMENPEKG
                     VWGLQFHPEVVHTAFGTEILSNFLFSVCGCVPDWDLGNWIDTMTEEIRRKVGPDRVIC
                     GLSGGVDSSVAAVLTARAIGDRLECIFVNNGLLRLNEAEQVLEQYKALNLKVHYVDAT
                     DVFLGALAGVSDPERKRKIIGERFVRVFEAEAEKIEGAKWLLQGTLYPDVIESGHKGK
                     NASVIKSHHNVGGLPEDMKLLVLEPLRDLFKDEVRSTGRMLGIPEEIVARQPFPGPGL
                     AVRCLGEITRERLDTLRRADAIFREELSGWEGVSSVWQSFVALLPVRTVGVMGDSRTY
                     SEAAVLRAVESHDGMTADWVRIPWEILDRTSRRICNEVHGINRVALDVTSKPPATIEW
                     E"
     gene            3145..>3324
                     /locus_tag="P5201_05700"
     CDS             3145..>3324
                     /locus_tag="P5201_05700"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRV98060.1"
                     /translation="MGGKGVSSGEGQKAQMSRGWFLPFLFIFLALAYLSYTVSLSLLK
                     PLAWAVLLSFTVYPVF"
BASE COUNT          591 a          983 c         1123 g          627 t
ORIGIN      
        1 tctgaaacgc cccctcccgg acgatgcgaa gcgcatgtta actctcctct ggggggacga
       61 agggcgcacg atcggtgagg cgctgcaggg agcgcttccg gcgatctccc tcgggagcca
      121 cggagcgctg atgaccgcct tcgccaacga cgccgacgga cggttcgcct tcgcgcagca
      181 ggcgatgggc tacgcgcgcc ccggagatgt cgcgttgggc atcagcacgt cggggaatgc
      241 ggagaacgtt cgtttcgccc tgatggcggc gaagagccgc ggcgcgtcga ccgtcgcgct
      301 gaccggtgcg gacggggggc tgctggcgaa gtatgcggac tgctgcatcc gcgttcccga
      361 cagaaggacg ttccgcgtcc aggagcacca cttgaggatc tatcatttcc tctgtgcgtt
      421 cgtcgagtcg gagctgttcg atgaatagca acgccgggcg gcgttctttc acaggggaaa
      481 ggggggaagt tgcatgaagc cgttttcact cgtacccgtc ttcaaggact atctctgggg
      541 cgggacgaaa ctcaagacgc ggttcaacaa gaggaccgag gtatcccctc tcgccgagag
      601 ctgggagctt gccgcgcatc cggacggaga ctgccgcata gcgaacggct ccttcgccgg
      661 aaccccgctg tcggagtttc tgcggaagtt ccctgaatcc ctgggaagcg gccgcgcctg
      721 cggagggggg ctccccgtac tgatcaagct gatcgacgcc agggaacctc tttcggtaca
      781 ggtgcatccg gacgacgtgt atgcgatgcg cacggagggg ggcgcgggga agacggagat
      841 gtggtacgta ctcgaccggg aacccggggc gtttctctac tgcggctttc aacgggatgt
      901 ttccagggag gagctgacgc ggcggatagc ggacggcacg gtgacggagg tgctgcacaa
      961 agcgcccgtg gagccgggag acgtgttctt catggaggct ggaacggtcc acgccatcgg
     1021 cgccgggata ctgatcgccg agatccagca gagctcgaac acgacgtaca gggtcttcga
     1081 ctacggccgc cgcggtcccg acggaaagga gcggccgctg cacatcgaaa aggcgctcga
     1141 cgtcgcccgc aggggacccg catgctccgt ccctccaggt agccggcctc ccgtcatctt
     1201 gcccggaagc acgctccgcc gtctcgcgag atgcgcgcac ttctccgtgg agcttcttga
     1261 gctttcggaa cactgcgagt accggacgga cgaaacatcc ttcctctctc tcctctgtct
     1321 cgaaggctcg gcgaggttgg ccgaggggga gtggggattc gatatcgcga agggagacag
     1381 cgtcttcgtc cctgcgcgga aaggatccgt cttccttgaa ggacgcggaa cttttctgct
     1441 gacgactgtt ccggacggag agctctgaga gggaacgggg cggttttcgc ggcgctttcc
     1501 gtttcctctt gtggaacggc gaggggcttt ctggtaagat acaacggggt gtgaaagccg
     1561 aactttccgg ccccggtttt tctatatttc agggggaatg gacttgtgaa caacatcgtc
     1621 atactcgatt gcggctccca gttcacgcag cttatcgcga gaagaatcag ggagctgaag
     1681 gtacacagcg agattctgcc gtgggacgct cccatcgagc gaatcaagag cgcggctctg
     1741 tctggcgtcg tcatctccgg cggaccgatg agcgtctccg acgagggcgc cccgacagta
     1801 ccggaggagc tgtttcagct cggcgttccg gtcctcggcg tctgttacgg gatgcagctc
     1861 gcgacgaagc tcttcggcgg cgtcgtcacg tcgggcagct cccgtgagta cggacgcacg
     1921 gcgatctccg tcaaggagga gtcggcgctt ttttccggca cagccgcttc gttcgaagtc
     1981 tggatgagcc acggcgatca catcgaccgt cttgctcccg gattccgctg cgtcgccgaa
     2041 accgcgaacg gcgttccggc ggcgatggaa aacccggaga agggcgtctg gggactccag
     2101 ttccaccccg aggtggtgca tacggccttc gggacggaga tcctttcaaa ctttctcttc
     2161 tcggtctgcg gatgcgtgcc cgactgggat ctcggcaact ggatcgacac catgacggag
     2221 gaaatccgcc ggaaggtcgg accggacagg gtgatctgcg gcctctccgg cggggtcgac
     2281 tcgtccgtgg cggcggtgct cacggcgcgg gccatcggcg accggctgga gtgcatcttc
     2341 gtcaacaacg ggctgttgcg cctgaacgag gcggaacagg tgctcgaaca gtacaaggcg
     2401 ctcaatctga aggtgcatta cgtcgacgcg acggacgtct tcctcggggc gctcgcaggc
     2461 gtctccgatc ccgagcggaa gcggaagatc atcggcgagc gtttcgtcag ggtcttcgaa
     2521 gcggaggcgg agaagataga gggcgccaag tggctgcttc agggaacgct ctaccccgat
     2581 gtcatcgaga gcgggcacaa ggggaagaac gcctcggtca tcaagagcca ccacaacgtc
     2641 ggcggcctgc ccgaagatat gaaacttctc gtactggagc ctctccgcga tctcttcaag
     2701 gacgaggtcc gctccacggg gcgtatgctc ggcatccccg aggagattgt ggcgcgccag
     2761 cccttccccg gtcccggcct cgccgtccgc tgcctcggag agataacgcg cgagcgcctt
     2821 gacacgctgc gccgggcgga cgccatcttc cgggaggagc tttccgggtg ggaaggcgtt
     2881 tcttccgtat ggcagtcctt cgtggcgctg cttcccgtca ggacggtcgg cgtgatgggg
     2941 gattcgcgca cctattcgga ggcggcggtg ctccgcgccg tggagtcgca cgacggcatg
     3001 acggccgact gggtgcggat tccctgggag attctcgaca ggacctcgcg gaggatatgc
     3061 aacgaagtgc acgggatcaa ccgggtcgcg ctcgacgtga caagcaagcc gccggcaacc
     3121 atcgagtggg agtaggcgac ggcgatggga gggaagggcg tttcgtccgg agaaggacag
     3181 aaggcgcaga tgtcgagagg gtggtttctc ccgtttctct ttatcttcct tgcgcttgcg
     3241 tacctctcat atacagtctc gctttctctg ctcaaaccgc tggcgtgggc ggtactgctt
     3301 tcgttcaccg tatatcctgt cttc
//
LOCUS       DAOTIL010000220         3311 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminobacteriaceae bacterium isolate AMR_MDS_3026
            NODE_34106_length_3311_cov_3.947789, whole genome shotgun sequence.
ACCESSION   DAOTIL010000220 DAOTIL010000000
VERSION     DAOTIL010000220.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868050
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminobacteriaceae bacterium (wastewater metagenome)
  ORGANISM  Aminobacteriaceae bacterium
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae.
REFERENCE   1  (bases 1 to 3311)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3311)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:19:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,224
            CDSs (total)                      :: 3,198
            Genes (coding)                    :: 3,184
            CDSs (with protein)               :: 3,184
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3311
                     /organism="Aminobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_34106_length_3311_cov_3.947789"
                     /isolate="AMR_MDS_3026"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:3034609"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..412
                     /locus_tag="P5201_05705"
     CDS             <1..412
                     /locus_tag="P5201_05705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012869717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HRV98061.1"
                     /translation="GGVVIIECPQRIPCNPCHTSCPTGAILPFEDINNTPKVNWEKCT
                     GCTLCVSACPGLACFVIDLTFSEENALYKLPYEMLPLPVKGQKVQCLDREGKSVGEGT
                     VENVTQPRKDRTHVVHVSAPKSLVTIFRSIRVVK"
     gene            412..702
                     /locus_tag="P5201_05710"
     CDS             412..702
                     /locus_tag="P5201_05710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014807728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(2Fe-2S)-binding protein"
                     /protein_id="HRV98062.1"
                     /translation="MSVTPENNEKVYVCRCEEVTLEEIDEWIDRGYDTVEELKRVLRV
                     GMGPCQGRGCQDILMREIAKKTGKPISEVAPATVRPPVKPIKIGLLAEGGEE"
     gene            702..1850
                     /locus_tag="P5201_05715"
     CDS             702..1850
                     /locus_tag="P5201_05715"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006301013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HRV98063.1"
                     /translation="MEWKSTAEVVIVGGGIIGVSTAYYLAKQGVKDIVLVEKGTICGG
                     STGRCGAGIRAQWGTEMNCRLGIAALDIFEQLDQELGMSVGLNQGGYLLVAYNEKELE
                     TLRNGMLLQNSLGIQSRMVTLTEAREICPGLAAEDAVGFFFHGRDGHADPFLTTFAYL
                     EAAKRLGVTCYRNTACTGVIVRDNTVQGIETTNGKISAPIVINCAGAYAQIVARMAGI
                     ELPNWAERHEIMITEPVAPGVCPCMLMSFSGNYYIQQRPHGSIICGMSPQGHPEDFEN
                     KTTWQFIEAMSKTLVKLLPRTKGIRIARQWSGLYDMTPDAQPVIGETDVKNFYHSTGY
                     SGHGFMLAPVAGKILAQHITGQKPDIDFSMLDYRRFSRGEHIRESNVV"
     gene            1886..2281
                     /locus_tag="P5201_05720"
     CDS             1886..2281
                     /locus_tag="P5201_05720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006301182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HRV98064.1"
                     /translation="MKFLKPLLDKCVMCGACMSACSKAWAKQDNPAMSRIAVKNVTGL
                     PDIRVCDQCGGCIAVCPTMALQRDKNGVVQIDRKKCTSCLICVEFCPSASMFFHADID
                     SPFKCVACGICVKACPEGALSIVEQKQEG"
     gene            2286..>3311
                     /locus_tag="P5201_05725"
     CDS             2286..>3311
                     /locus_tag="P5201_05725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006583323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016625 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, iron-sulfur
                     protein as acceptor [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde ferredoxin oxidoreductase N-terminal
                     domain-containing protein"
                     /protein_id="HRV98065.1"
                     /translation="MEQMKLLTEWEYTPAKLNKGYTRQFLSVDLGKADGAFTCETHPI
                     SDEMVEKFTGGRGFGLRILWDKVTSKSKWNDPENAMVISGGPLSGTTQYPGMGKFYAV
                     FLSPLTQQTYNSNAGGYFGPFLKAAGFDALSVSGKAQKDVVVYIDGDEGAVRIFEWAP
                     ENRNSYTVTEALHEFFAENEEDKRNISVVSAGEAAEHSWWGAMNVSFYDPRRALPRLK
                     QAGRGGGGTVLRDKKILALVVKKKGFSGTENAPADLSTLQKVGTKLHREIRQNDDVQC
                     LMRKVGTAHLNLVMNDYDILPVNNFKFGRNEDIHNIAPDAYEKLFTQGYADGCWYGCS
                     MACAKAVD"
BASE COUNT          841 a          852 c          922 g          696 t
ORIGIN      
        1 gggcggtgtc gtgataattg aatgccctca gagaatcccc tgtaatccct gccatacaag
       61 ctgtcctacg ggagctattc ttccgtttga ggatatcaat aatactccga aagttaactg
      121 ggaaaagtgc acgggttgca ctctctgcgt ctcagcctgt cccggactcg catgcttcgt
      181 tattgatctt acgttctcag aggagaatgc actgtacaag ctgccgtacg aaatgctgcc
      241 ccttccggta aagggacaga aagtccagtg ccttgaccga gaaggaaagt ccgtcggcga
      301 aggaaccgtc gagaacgtta ctcaaccgcg caaggatagg acgcacgtcg ttcatgtctc
      361 tgcgccgaag agccttgtaa cgatttttcg ttcgataagg gtggtgaaat aatgagcgtt
      421 acgcctgaga ataacgaaaa ggtctatgtc tgccgatgtg aagaggtcac gctcgaagag
      481 atcgacgagt ggattgatcg cggatacgac accgtcgagg aattgaaacg cgttcttcga
      541 gtcggaatgg gcccatgcca gggacgcggg tgccaggata tactcatgag ggagatcgcg
      601 aaaaaaacag gaaagcccat ttcagaagta gccccggcaa cggttcgccc ccccgtaaaa
      661 cctattaaga tagggctcct tgcagaagga ggtgaggagt gatggagtgg aaatcaaccg
      721 ctgaggttgt aatcgtaggc ggaggtatca tcggagtttc caccgcctac tatcttgcga
      781 aacaaggggt taaagacatc gtcctcgttg aaaagggaac tatctgcgga ggttctacgg
      841 gacgttgcgg cgcaggaatc cgtgcgcagt ggggaaccga aatgaactgc cgtctgggta
      901 ttgccgctct ggacatattc gagcagctcg accaggaact tgggatgtcc gtcggattga
      961 atcagggagg gtatctcctt gttgcgtata acgaaaagga gctggaaacg ctccgtaatg
     1021 ggatgctcct ccagaactca cttggaatac agtcgcgcat ggtcactctc accgaagccc
     1081 gcgaaatctg tccgggccta gcagcggaag acgcggtcgg tttctttttc cacggcagag
     1141 acgggcatgc cgacccgttc cttacgacat tcgcttacct tgaagctgcc aagcgcctcg
     1201 gcgtaacatg ctatagaaac actgcatgca ctggagtcat cgtccgggac aacactgtcc
     1261 aggggatcga aaccactaac ggaaaaatta gcgccccgat cgtcatcaac tgcgccggag
     1321 catatgctca gatagtcgcc cgtatggcag gtatcgagct tcccaactgg gccgagcgcc
     1381 atgagataat gatcactgaa ccggtcgctc cgggagtctg cccctgcatg ctgatgagtt
     1441 tctccggaaa ctattacatt cagcagcgtc cgcatggctc catcatctgc ggtatgagtc
     1501 cccaagggca ccccgaggat ttcgagaaca aaacgacatg gcagttcatc gaagcaatgt
     1561 cgaaaacgct cgtcaagctc cttccgagaa caaaaggcat aaggatagcg cggcagtggt
     1621 caggactgta cgatatgact ccagatgcgc agccggtgat cggagagacc gacgtcaaga
     1681 atttctatca ctctacggga tattccggac acggattcat gctcgccccc gtagccggga
     1741 aaattctcgc ccagcatatc acaggacaaa aaccggacat cgatttttcg atgctcgatt
     1801 atcgtcgctt cagccgcgga gagcacatca gagaatcaaa cgtggtatga tggaaagata
     1861 gatcaccaga aaaggagtgt ttgttgtgaa gtttctgaaa cctttactcg acaagtgcgt
     1921 catgtgcgga gcatgtatgt ctgcatgctc gaaggcgtgg gccaagcagg acaatcccgc
     1981 gatgtcgcgc atcgccgtca agaacgtgac agggctgccc gacatcaggg tatgcgatca
     2041 gtgcggcggc tgcatcgccg tctgtccaac aatggcgctg cagagggaca agaatggagt
     2101 cgtccagata gatcggaaaa agtgcacatc ctgcctcatt tgcgtcgagt tttgtccctc
     2161 cgcctccatg ttcttccatg cggacataga ctctccgttc aagtgcgtcg cctgtggaat
     2221 ttgtgtaaag gcgtgccccg agggcgctct gtctatagtc gagcaaaaac aggagggata
     2281 ggagaatgga acagatgaag cttctcaccg agtgggaata cactcctgcg aaacttaaca
     2341 aagggtatac acgccagttc ctttctgtgg atctcggcaa ggccgacggc gcgttcacct
     2401 gtgaaacaca ccctatttcc gacgagatgg tggagaagtt taccggcgga cgcggtttcg
     2461 gccttcgtat cctctgggac aaggtcactt caaagagcaa gtggaacgac ccggaaaacg
     2521 cgatggtcat ctccggaggg ccgctttccg gcaccacgca atatccgggc atgggcaagt
     2581 tttacgcggt attcctttcg ccgcttacgc aacagacgta caacagcaat gcaggagggt
     2641 acttcgggcc attcctgaag gctgccgggt ttgacgccct ttctgtatct ggcaaagcgc
     2701 agaaagacgt tgtagtgtat atcgacggcg atgaaggcgc ggtacgtatc ttcgaatggg
     2761 cgcctgagaa ccgtaactcc tataccgtca cggaagcgct tcatgagttc ttcgcggaga
     2821 acgaagaaga caagcgcaac atctcggttg tctcggctgg agaagctgcg gaacacagct
     2881 ggtggggggc tatgaacgtc agtttctatg acccaagaag agcgcttcct cgcttgaagc
     2941 aagctggacg cggcggcggc ggcactgtgc tcagggacaa aaagatcctc gcgctcgtcg
     3001 tcaaaaagaa gggcttttct ggtaccgaaa acgccccggc tgatctgtcc actttgcaga
     3061 aagtcgggac aaaactccac cgtgaaattc gccagaacga cgacgtacag tgtctcatga
     3121 gaaaagtggg cacagcgcac cttaacctcg ttatgaacga ctacgacatt cttccggtga
     3181 acaacttcaa attcggaaga aacgaggaca ttcacaatat cgctcctgac gcctacgaaa
     3241 aactattcac ccagggatac gccgacggct gctggtacgg ctgttcaatg gcctgcgcaa
     3301 aagctgtcga c
//
LOCUS       DAOTIL010000221         3310 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminobacteriaceae bacterium isolate AMR_MDS_3026
            NODE_34128_length_3310_cov_3.580338, whole genome shotgun sequence.
ACCESSION   DAOTIL010000221 DAOTIL010000000
VERSION     DAOTIL010000221.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868050
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminobacteriaceae bacterium (wastewater metagenome)
  ORGANISM  Aminobacteriaceae bacterium
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae.
REFERENCE   1  (bases 1 to 3310)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3310)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:19:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,224
            CDSs (total)                      :: 3,198
            Genes (coding)                    :: 3,184
            CDSs (with protein)               :: 3,184
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3310
                     /organism="Aminobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_34128_length_3310_cov_3.580338"
                     /isolate="AMR_MDS_3026"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:3034609"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            89..919
                     /locus_tag="P5201_05730"
     CDS             89..919
                     /locus_tag="P5201_05730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014806604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoacid:ferredoxin oxidoreductase subunit
                     beta"
                     /protein_id="HRV98066.1"
                     /translation="MPSQEVFNWLRTRFFPHIWCPGCGHGIIMHSLLRALVDLKKKKT
                     ETVIASGIGCSSRMVGYIDACTVHTTHGRSLAFATGIKLANPELTVVDVMGDGDCTAI
                     GGNHFIHACRRNIDITAIVMNNNIYGMTGGQVSPTTPVGSFATTSPYGAIDPSFDICK
                     LAEGAGASYVARTTIANPKQAEQFIKAGINNKGFSVVEILTHCHTQFGRRNKRRTPID
                     NVNFFKANSVPLNKAQEMSPEELAEKIVVGEFVKREIPEYTAQYLSIIERVGGLKNGK
                     "
     gene            909..1472
                     /locus_tag="P5201_05735"
     CDS             909..1472
                     /locus_tag="P5201_05735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005661979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016903 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoacid:acceptor oxidoreductase family
                     protein"
                     /protein_id="HRV98067.1"
                     /translation="MASRYEIRFAGSGGQGVILAAVVAGEAAALFEEGLSVVQTQSYG
                     PEARGGKSKAEVVISTEPIDYPKTMKPNLQVILTQQAAEEYAAETLPGGRIIYDDFFV
                     TSFPQVDANIYYLPIVRTAREKIGKEIVTNMVALGAVARVLELEKIVRPESLKKAILA
                     KVPEGTKQLNAQAFDEGYLLFKNSVHL"
     gene            1691..3058
                     /gene="rlmD"
                     /locus_tag="P5201_05740"
     CDS             1691..3058
                     /gene="rlmD"
                     /locus_tag="P5201_05740"
                     /EC_number="2.1.1.190"
                     /inference="COORDINATES: protein motif:HMM:TIGR00479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008649 - rRNA methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uracil(1939)-C(5))-methyltransferase
                     RlmD"
                     /protein_id="HRV98068.1"
                     /translation="MYEKGASVLLRTEKISSDGSGIARTPEGFVIFIPGALPGETLKA
                     TVMLRKKEYAIAFPDELLEPHPLRRQPFCPVFNSCGGCQLQHASYALQLELKRAIVQD
                     AFERIYKRPFPPVTSCNPSPEQIRYRNKTSLPVRNREGKTVMGYFARRSHDVVPIKSC
                     PVAAQHIDRAFSAVAESLPVLGLVPYNEKNGKGMLRHAIFRQSIENNDTLVSFVLADT
                     LSSRKKAIFEERLLPALQEALPNLRSLTLNINSARNNVIVGPKTEVLFGDGLIEETLA
                     SFRFEYDTTAFFQVNSRQACILYEFVVDLAGLTGTERVLELFSGIGTLTSFLAARSLH
                     VTAVEEWDSSVRMMRSNLSRNGVGEKTAIIAGAVEKVAPSLEGTFDVIVLDPPRTGCD
                     ASVLHKVLSLNPRRIIYVSCNPATLARDAGILTDQGFVLDTLTCFDLFPQTVHVETVA
                     RFFRI"
BASE COUNT          869 a          847 c          807 g          787 t
ORIGIN      
        1 aaagcgaaag tcgtggcaca gaaccttgta aacggcgagc tgttcaaacc cgatgaaatt
       61 ctctccgtca tcgaggaggt ggcctaatat gcctagtcag gaagtattca actggctccg
      121 cacgcgcttt ttccctcata tttggtgccc cggctgcgga cacggtatca ttatgcactc
      181 tcttttaagg gcgctggtcg accttaagaa aaagaaaacg gaaacggtca ttgcgtccgg
      241 aatagggtgt tcgagcagaa tggtcggcta cattgacgca tgtactgtac acaccacgca
      301 cggccgctcc ctcgctttcg caactgggat caaactcgca aatccggagc tcacagttgt
      361 tgatgttatg ggagacggcg actgcaccgc tattggcggt aaccacttca tccatgcgtg
      421 caggagaaat atcgacataa ccgccatcgt tatgaacaat aacatctacg gtatgactgg
      481 cggtcaggtt tcgccgacta cacccgtcgg aagctttgca acgacgagcc cctacggcgc
      541 aattgatcct tcctttgaca tttgcaaact cgccgagggc gccggagcat catacgttgc
      601 aagaaccacc attgccaacc cgaaacaagc agagcagttt attaaggcag gcataaacaa
      661 taagggattt tctgtcgtcg aaatcctgac gcactgccat actcaattcg gcagaagaaa
      721 caagcggaga acacctattg ataacgtgaa tttcttcaag gccaattctg tgcctttgaa
      781 taaggcacag gagatgtctc ccgaggagct cgccgaaaaa atcgtcgtcg gcgaattcgt
      841 caaacgagag attccggagt acacggcgca gtatctctcc ataatcgaaa gagtaggggg
      901 gctgaaaaat ggcaagtagg tacgaaatcc gtttcgccgg ttccggcgga cagggcgtca
      961 ttctcgcagc cgtcgtcgca ggagaagccg cagctctttt cgaggagggg ttgagcgttg
     1021 tccagacgca gtcttacggt ccggaggcca ggggaggcaa gtcgaaagcg gaagtagtca
     1081 tctccacaga accgattgac tacccgaaaa caatgaagcc caaccttcaa gttattctca
     1141 cgcagcaggc agcagaggag tacgcagcgg aaacactccc cggcgggcgt attatttatg
     1201 atgatttttt cgtcaccagc ttcccgcagg tggatgcgaa tatttactat cttcccattg
     1261 tcaggactgc gcgtgagaag ataggcaaag aaatagttac gaacatggta gctttgggag
     1321 cagtggcccg cgtccttgag ctcgaaaaaa tagtgcgacc ggagtcccta aagaaagcga
     1381 ttcttgcgaa ggttcccgaa ggaacaaaac aactcaacgc ccaggctttt gacgaggggt
     1441 atctcctgtt caaaaacagc gtgcaccttt aaaaaacact ttctccataa tgaacgccct
     1501 tcttttcgta aggagggcgt tcaatttttg agcctttctc cacaccaaat acaacgtaat
     1561 agcgaggaat acgcccaacg aaacttggaa gcctttttct gctctgaacc tttccctcac
     1621 tcgaaagact ttttccgctc tcttccgtgt ataatgtggt ctgtgcgcaa aaaaagagga
     1681 gatgatccga gtgtacgaga aaggcgcttc cgtccttcta aggactgaaa agataagcag
     1741 cgacgggagc ggaattgcgc gcactccgga aggttttgtt attttcatcc cgggggcgct
     1801 ccctggagaa acgctaaaag ctacagtcat gcttcgaaaa aaagagtatg cgattgcctt
     1861 ccctgatgaa ctgcttgaac cgcaccctct tcgccggcag cccttctgcc cggttttcaa
     1921 ttcttgcgga ggatgccagt tacagcatgc ctcgtacgct cttcagctgg aactgaaacg
     1981 tgctattgtc caagatgctt ttgaacgtat ctataaaaga ccgtttcccc ccgttacctc
     2041 atgtaatccg agtccagagc agataaggta cagaaacaag acttcccttc cggtacggaa
     2101 ccgagaagga aaaactgtta tggggtattt tgcaagaaga agtcacgacg tagtaccgat
     2161 aaaaagttgt cccgttgccg cgcaacatat tgacagggcc ttttcagcgg tggccgaaag
     2221 tctgccggtt ctcggtctcg ttccatataa cgagaaaaac gggaaaggca tgcttcgcca
     2281 tgcaattttc cgtcagagta tcgaaaacaa cgacaccctt gtttccttcg tccttgccga
     2341 tacactctct tcccgcaaga aagcaatttt cgaagaacgc ctgctcccgg cgcttcagga
     2401 agcgcttccc aacttacgca gcctgactct gaacatcaac tcggcacgga acaatgtaat
     2461 cgtcggcccg aaaacagagg tgctcttcgg agacggcctt atagaagaaa ccctcgcctc
     2521 ttttcgattt gagtacgata caacggcatt cttccaagtc aactcgcgtc aggcatgcat
     2581 cctgtatgaa ttcgttgtgg atctagccgg cctcaccggc actgaacgag tgctcgaact
     2641 ctttagcggt ataggtactt taacttcttt tctcgccgcc agaagtcttc atgtaacagc
     2701 ggtggaggag tgggactctt cggtacgaat gatgcggagc aacctctccc gcaatggagt
     2761 aggcgaaaaa accgcgatta ttgcaggagc agtggagaaa gtagcgcctt ctctcgaagg
     2821 tactttcgat gtgattgtcc ttgatcctcc gcgcacaggc tgtgacgctt cggtgctgca
     2881 caaggtcctt tcgttgaatc cacgccggat tatctatgtt tcgtgtaatc cggcaaccct
     2941 ggcaagggat gccggtattc tcactgatca aggtttcgtc cttgatactc tgacatgttt
     3001 cgatttattt ccccagaccg ttcacgtcga aaccgtggcg cgattctttc gtatctaatc
     3061 accacgtgtt tttcattggc cagagggaaa acgttacccg ggcaacgcag ttatttctaa
     3121 gaacgcatac tactgagaat atcctctcca gtctgtgttt caacaaatga aatcaggtgt
     3181 gtcaggaaaa agatgttgcc aaaacaggat acgacggata taataccatc gttcaagcaa
     3241 aagagggcgg acatagctca gctggtagag cattagcttc ccaagattta ttttgtcaat
     3301 cattcacccc
//
LOCUS       DAOTIL010000222         3303 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminobacteriaceae bacterium isolate AMR_MDS_3026
            NODE_34213_length_3303_cov_3.679187, whole genome shotgun sequence.
ACCESSION   DAOTIL010000222 DAOTIL010000000
VERSION     DAOTIL010000222.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868050
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminobacteriaceae bacterium (wastewater metagenome)
  ORGANISM  Aminobacteriaceae bacterium
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae.
REFERENCE   1  (bases 1 to 3303)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3303)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:19:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,224
            CDSs (total)                      :: 3,198
            Genes (coding)                    :: 3,184
            CDSs (with protein)               :: 3,184
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3303
                     /organism="Aminobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_34213_length_3303_cov_3.679187"
                     /isolate="AMR_MDS_3026"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:3034609"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..394
                     /locus_tag="P5201_05745"
     CDS             <1..394
                     /locus_tag="P5201_05745"
                     /inference="COORDINATES: protein motif:HMM:NF020052.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0006304 - DNA modification [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="type I restriction-modification enzyme R subunit
                     C-terminal domain-containing protein"
                     /protein_id="HRV98069.1"
                     /translation="PRTGEPAFWDGDILWATPKDLSDLDRKYLNDTARKITTAGLKSC
                     PQQIRFVETIIDQLTAHGVMEPSALYEPPFTRIDSGGPDALFDGRENVVAGIFETLDA
                     INSGLYDEGTSLRVARGKSGGTMHCESV"
     gene            complement(436..1173)
                     /locus_tag="P5201_05750"
     CDS             complement(436..1173)
                     /locus_tag="P5201_05750"
                     /inference="COORDINATES: protein motif:HMM:NF020419.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyl transferase AbiEii/AbiGii toxin
                     family protein"
                     /protein_id="HRV98070.1"
                     /translation="MEKNLRPAGIIPSEKSAGWEAPEQRALLVEIFKIPLFSESFFLG
                     GGTCLSVMYFGHRQSQDLDLFTVDEISIPVAWGMLARRLPRRGYALETTISISEGFAS
                     GVFQKNGVMTKVDLVQDMFVEDLRREQVVLDGVPIRVDNFGNLVTGKFSAFLSRGEPK
                     DIIDLSRILNHAKDTGQFIPFLERLLTETSKRDALADDDTLLSEIFTKIERQHSDLFH
                     PEIARMVLAFLETLQRRKKALSEGEHS"
     gene            complement(1089..1472)
                     /locus_tag="P5201_05755"
     CDS             complement(1089..1472)
                     /locus_tag="P5201_05755"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRV98071.1"
                     /translation="MEKVSPENFSFDIIRFFRYPKIFWDDKEIRNASPSELKRLIDRL
                     RSSPVPLDRLRFKAFVRRMIERGSASDALLFLTPDEIREFLEQESVYCVDERMESLWK
                     KISGLPESFQVKKVRAGKPRSSGLS"
     gene            1650..1787
                     /locus_tag="P5201_05760"
     CDS             1650..1787
                     /locus_tag="P5201_05760"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRV98072.1"
                     /translation="MRLDADGPDALFDGRENVVANIFETLDAINSGLYDERTSLRVAR
                     K"
     gene            1968..2978
                     /locus_tag="P5201_05765"
     CDS             1968..2978
                     /locus_tag="P5201_05765"
                     /inference="COORDINATES: protein motif:HMM:NF022732.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3089 domain-containing protein"
                     /protein_id="HRV98073.1"
                     /translation="MPRRFSFILAALLTLVLIPYFPGISCAEPGYSFDPALAPSSPDY
                     SLPKSWAVLPEEPLPYPVDIFFVHPTTYNGDENWNQSLEGESADPKVRGVMKSQAGVF
                     STSACLYAPYYRQANLAVLGTSEESPARRSLDVAYSDVEAAFDAYMTFWNKGRPFILA
                     GHSQGSEHLLHLLERRFGDAELRKKLVAAYIIGWSVTKDDLARYPFLKIAESPDQTGS
                     IVSFNTQGPNPGYSIVREGAVGVNPLTMTITGEVADADQNLGAVFFPDKGVMEIPHYT
                     GGQTVGGAFVIPTPSNINDLDAMKIPEFYHPYDYTFFYRNLQQNAELRTRTYLESHGI
                     KN"
     gene            complement(3099..>3303)
                     /locus_tag="P5201_05770"
     CDS             complement(3099..>3303)
                     /locus_tag="P5201_05770"
                     /inference="COORDINATES: protein motif:HMM:NF015949.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF364 domain-containing protein"
                     /protein_id="HRV98074.1"
                     /translation="AMVAGAREICVIGPSAPLLPDVFKKRGITLLSGRRFLDADRLLR
                     IVSEAGGTKCFGPVSLKVNIQLK"
BASE COUNT          756 a          881 c          887 g          779 t
ORIGIN      
        1 acccagaaca ggcgaacctg ccttttggga tggagatatt ctttgggcta ctccaaagga
       61 tttaagtgac cttgatcgga agtatttgaa cgatacagcc cgcaagatca cgacagctgg
      121 tttgaaaagc tgcccgcagc agatccgctt cgtcgaaacg atcatcgacc agcttacggc
      181 ccacggcgtg atggagccgt ccgccctcta cgagcctccg ttcacccgca tcgactctgg
      241 cggccccgac gcactcttcg acggcaggga gaacgtcgtc gccggcatct tcgagacgct
      301 ggacgccatc aattccggcc tttacgacga gggcacaagc ctccgcgtcg cgaggggaaa
      361 gagcggggga acgatgcatt gcgagagcgt ataatctgtt aatggcaggt gtttgtggtt
      421 tgaaaaagag agacttcagg aatgctctcc ctctgaaaga gccttttttc tgcgctgtaa
      481 agtttctaaa aaagcaagaa ccatacgggc gatttccggg tgaaacaggt cggagtgctg
      541 tcgttctatc ttggtgaaga tttcgctgag aagagtgtcg tcgtcggcaa gagcgtctct
      601 tttcgatgtt tctgtcagca ggcgttccag gaacgggata aactgaccgg tatcctttgc
      661 atgattcagt atacgcgaga gatcgatgat atcctttggt tctcctctgc ttagaaaggc
      721 ggagaatttc ccagtgacaa gattaccgaa attgtccact cgtataggaa ctccgtcgag
      781 aacgacctgt tcacggcgca gatcctcgac aaacatgtcc tgaacaaggt ccacttttgt
      841 catcacgcca tttttttgaa agactccgga cgcgaatcct tcgctgattg aaatcgttgt
      901 ttccagggcg tagcctcttc tgggaagacg cctggcgagc atcccccacg caacagggat
      961 tgaaatctca tcaaccgtga acaggtcgag atcctggctt tgacgatggc cgaaatacat
     1021 tactgagagg caggtaccgc ctccgaggaa gaagctttca ctgaaaagcg gtatcttgaa
     1081 tatctcaact aagagagccc gctgctccgg ggcttcccag cccgcacttt tttcacttgg
     1141 aatgattccg gcaggccgga gatttttttc cataggctct ccatcctttc atccacgcag
     1201 taaacgcttt cctgctcaag aaactccctg atctcgtccg gtgtcagaaa gagcagcgcg
     1261 tcgctggcgc tgcctcgctc gatcattctg cggacgaacg ccttgaagcg gagtcggtcc
     1321 aatggaaccg gagaagaacg cagtctgtcg atcaggcgtt ttagctccga tggcgatgcg
     1381 ttccggatct ctttgtcgtc ccagaagatt ttcgggtagc ggaaaaaacg tatgatatca
     1441 aacgaaaagt tttccggcga aaccttttcc atcgtctcaa cctcctttcc gatggtcggg
     1501 aactctatga ttcgagtata tcatgtcgga gactgttcaa aaacgttgcc gaggggcggc
     1561 atccaggttg tgcgatagac tcttgaatcc ggcggtcttg cttctataat acagctatgt
     1621 tggagtgggg cgaacgagat ttttcatcgt tgcgacttga tgcagacggc cccgacgccc
     1681 tcttcgacgg cagggagaac gtcgtcgcga acatcttcga gacgctcgac gccatcaatt
     1741 ccggcctgta tgacgagcgc acaagcctcc gcgtcgcgag gaaataattc agtaaaaggg
     1801 ggaggggcat tatgaacggc tggtctgtga gaggaggccc gaaaaggttc cggcttgccg
     1861 ggcgccggtt ttgacggaag aaagtacaca aaacattgga gaagattcga tttatgctcg
     1921 atcgctgcat ctaaaactgt acatacaaat taaaggagtg aatactgatg cccagaaggt
     1981 tttcttttat tcttgccgcg ttgcttactc tcgtcctgat accatatttc ccaggaattt
     2041 cctgtgcgga accggggtat tctttcgacc cggctctggc tccttcctcc ccggactatt
     2101 ccttgccgaa aagctgggcc gttctgcccg aagagcctct tccctatccg gtggatatct
     2161 tttttgttca ccctacgacg tataacggcg atgaaaattg gaaccagtcc cttgagggcg
     2221 agagcgccga tccaaaggta cgaggggtta tgaagagcca ggcgggagtt ttttcaacat
     2281 cggcttgtct ctacgcccca tactaccgtc aggcgaacct ggctgtgctg ggcacatctg
     2341 aggagagccc ggccagaagg tcccttgacg tggcctactc ggacgtggag gctgccttcg
     2401 acgcctacat gactttttgg aacaagggga ggccctttat cctggcaggt cacagtcaag
     2461 gatcggaaca cctgctgcat cttttggaac ggcgcttcgg cgatgctgaa ctgaggaaga
     2521 agctggttgc ggcctatatc atcggctggt cggtcacgaa agacgacctt gcccgatatc
     2581 cttttttaaa gatagccgag agcccggacc agactgggag catcgtcagc ttcaacacac
     2641 aaggacccaa tcccgggtac agcatcgttc gggaaggggc cgtgggcgtc aatccgctca
     2701 ccatgaccat caccggggag gttgcggacg cggatcagaa cttgggggcg gtattttttc
     2761 ccgacaaggg agttatggag attccccact atacaggagg gcagaccgtc ggcggggctt
     2821 tcgtcatccc gacgccctcc aacatcaacg accttgacgc catgaaaatt ccggagtttt
     2881 accaccctta cgactatacg ttcttctaca ggaatcttca gcaaaacgcc gaacttcgga
     2941 cacgtacgta cctggaatcg catggcataa aaaactgaca atcctccggc ggcccgggag
     3001 ttgaactttt ttccccgttt actcccgggc cgcttccctt ttgaaaaact ttgtattgta
     3061 attttaagaa ggcgcttcgc cagtggagca gttttcttct atttcagctg gatattgact
     3121 ttcaggctga ccgggccgaa gcacttggtt cccccggctt cgctgacgat gcgaagcagc
     3181 cggtcggcgt cgagaaaacg gcgtccggac agcagcgtta tgccccgttt tttgaacacg
     3241 tcgggcagca gaggcgccga agggccgatg acgcagatct ccctcgcgcc tgccaccatc
     3301 gcg
//
LOCUS       DAOTIL010000223         3297 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminobacteriaceae bacterium isolate AMR_MDS_3026
            NODE_34290_length_3297_cov_3.997224, whole genome shotgun sequence.
ACCESSION   DAOTIL010000223 DAOTIL010000000
VERSION     DAOTIL010000223.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868050
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminobacteriaceae bacterium (wastewater metagenome)
  ORGANISM  Aminobacteriaceae bacterium
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae.
REFERENCE   1  (bases 1 to 3297)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3297)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:19:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,224
            CDSs (total)                      :: 3,198
            Genes (coding)                    :: 3,184
            CDSs (with protein)               :: 3,184
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3297
                     /organism="Aminobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_34290_length_3297_cov_3.997224"
                     /isolate="AMR_MDS_3026"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:3034609"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            430..1416
                     /locus_tag="P5201_05775"
     CDS             430..1416
                     /locus_tag="P5201_05775"
                     /inference="COORDINATES: protein motif:HMM:NF024808.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HRV98075.1"
                     /translation="MKKRVFSLLAVLVLVALMVPAFASAKEVMLYSSMKDSQLSALKD
                     AFIAKYPDIRMDYYTAGTGSVMTKLATEQQAGGIRADVIWVGDPTNYISFKEQGLLYA
                     YESPEAATMPANFKDPDNCYVAGRVIMLGFVYNTSALSANDVPKTWKELAEFKGYTAM
                     TDPTFSGTTLYTVAGLVQHPDFGWDFLQKLKDKGMLLERGSSAVVTKVGSGEYDVSVG
                     VDYIARTMIAQGSPIAFAYPQDGIPIILSPIAIVKNTANIEEAKLLFDFILSLEGQGI
                     LMKEYSAPVRPELSLEGALSVEEAAKKILPVDDNRLVAEKDELLKKFDSIFK"
     gene            1601..2689
                     /locus_tag="P5201_05780"
     CDS             1601..2689
                     /locus_tag="P5201_05780"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HRV98076.1"
                     /translation="MAGVTFKNITISYDAKVIIDNLSLEVDDGECFAILGPSACGKTT
                     LMRALCGFKNLDSGQIYIGDKLVGTKERWKSVAPEKRNVGVVFQDYAVWPHYSVFENV
                     YYPLKKRKVPKDEAKKRALDAIEQVRMKGYEQRMPAQLSGGQQQRVALARALVSSNQL
                     MVLDEPISNLDANLREEMRFEIKELQRRTGITVIYITHDQEVALAIADRMAILDRNGY
                     IRQIGVPEDVYRNPKDSYIYKFLGVSNFIPVERRGQEYVLDGTEGKQAFPYEIPEELR
                     AERLFAACRPVDITVGGNGPVQGFVKHVVYLGNLFEYRVLLGGREIRVQQDSHEAFRA
                     GVPEEGRPCALSFRNLRFYQEDEEGVAS"
     gene            2686..>3297
                     /locus_tag="P5201_05785"
     CDS             2686..>3297
                     /locus_tag="P5201_05785"
                     /inference="COORDINATES: protein motif:HMM:NF012738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HRV98077.1"
                     /translation="MKMFGRREGKLDLNQILMLICLTILIITVAMPMIMIVYNTFFYG
                     FTFDWSMFTNVVMDPSNVDAMWNTIVIAFWVTVVGTIVGVFYAWLIGRTNIPLKGLMK
                     TLFVIPYMFPPFIGAMAWGLLLSPRSGYINKLWMLMMGTREPLMNINSIAGIVFVEVC
                     YYFPFVFLQVVSALERMDPTLEESARISGANQLYVIRKITLPLV"
BASE COUNT          690 a          898 c         1014 g          695 t
ORIGIN      
        1 gcgaacgtag aaataggtat cgggatggtc tttggccagc gcgccgccga cgattccgcc
       61 gaccccgcct attccgaaca ctgcgatttt catagtatga cacctcccaa gttctgcaag
      121 taagcgcgtg aagcgcgctt ttccggcttt cagtatatgc cgttttcttc ctcggatcaa
      181 atcgggaccg cgccctatca gtttccatac gggagcaaca cgacgcacgg agccgggaaa
      241 agcatgtgtg agcgacgccg cggacggcgg cgaaaggacc tccgttgcat caatactcca
      301 gcaacactgg caaacggggc gtgtgaatgt tacaattctg taacagggca acgcacgcat
      361 gtacgcaagg ggtcgaaaag ggctctttgc cgcgttgcca agcattttat acacaaaggg
      421 ggcaatacaa tgaaaaagcg tgtttttagt cttctggcgg ttcttgtact tgttgcgctg
      481 atggttcccg cgttcgcttc ggcgaaagag gtgatgctgt actcttcgat gaaggactct
      541 cagctgtccg cgttgaaaga cgcctttatc gcgaagtatc cggatatccg tatggactac
      601 tataccgcgg gtaccggcag cgtgatgacg aagctggcca ccgagcagca ggccggaggc
      661 atccgggccg acgtcatctg ggtgggagat ccgacgaact atatctcctt caaagagcag
      721 gggcttctgt acgcgtacga gtctcccgag gcggcaacca tgcccgcgaa cttcaaggat
      781 ccggacaact gctacgttgc gggacgtgtg atcatgctgg gctttgtgta caacacgtcg
      841 gcgctttccg ccaacgacgt gcccaagacc tggaaggagc tcgcggagtt caaggggtac
      901 accgcgatga ccgacccgac cttctccggc acaacgctct acacggtcgc cggactggtg
      961 cagcatcccg atttcggttg ggacttcctg cagaagctga aggacaaagg tatgctgctt
     1021 gagagaggct ccagcgcggt ggtgacgaag gtcggttccg gcgagtacga cgtgagcgtg
     1081 ggcgtggact atatcgcccg caccatgata gcccagggct ctcccatcgc gttcgcctat
     1141 cctcaggacg ggattcccat catcctgagc cccatcgcga tcgtcaagaa caccgcgaac
     1201 attgaggaag cgaagctgct cttcgacttc atcctctccc ttgaaggtca gggaatcctg
     1261 atgaaggagt acagcgcccc cgtccgtccg gagctgagcc tcgaaggggc cctctccgtg
     1321 gaagaggcgg cgaagaagat tcttcccgtg gacgacaatc gccttgtggc ggagaaggac
     1381 gagctgctga agaagttcga cagcattttc aagtaggtcg acagagtttc gcggggaggg
     1441 gcctgaaacg cccttcccct tcagtaccat cgaagcggcg tcgccggccg gaggaatatt
     1501 tgggaagcgg cctttcaggc gttctcccgg agattgtccg gccgcttttt tatacccgct
     1561 tttcgatgca ttttttggaa agcagtgcga ggtgaacgta atggccggag taacatttaa
     1621 aaacatcacc atcagctacg atgcaaaggt catcatcgac aacttgtcgc tggaggtcga
     1681 cgacggggag tgctttgcca tcctcggtcc gtccgcctgc gggaagacca cgctgatgcg
     1741 ggcgctttgc ggcttcaaga atctggattc ggggcagatc tacatcggcg acaagctggt
     1801 cggaaccaag gaacggtgga agtccgtcgc cccggagaag cgcaacgtcg gggtggtttt
     1861 tcaggactat gcggtgtggc cccactacag cgtgttcgag aacgtctact accccctgaa
     1921 gaagcggaag gttccgaagg atgaggcgaa aaagagagcg ctggacgcga tcgagcaggt
     1981 gcgcatgaag gggtacgaac agcgtatgcc cgcccagctc tcgggggggc agcagcagcg
     2041 ggtcgctttg gcgcgcgcgc tggtttcttc gaatcagctg atggtgctcg acgagcccat
     2101 ctccaacctg gacgcgaacc tgcgcgagga gatgcgcttt gagatcaagg agctgcagcg
     2161 gcgtacgggc atcacggtga tctacatcac ccacgatcag gaggtggctc tggccatcgc
     2221 cgaccggatg gccattctgg accggaatgg ctacatccgg cagataggcg taccggagga
     2281 tgtctacagg aaccccaagg acagctatat ctacaagttt ttgggcgtct ccaacttcat
     2341 cccggtcgaa cggcggggac aggagtacgt cctcgacggt acggagggga agcaggcgtt
     2401 tccgtatgaa attcctgaag agctccgtgc ggagaggctg ttcgccgcgt gccgccctgt
     2461 ggatatcacg gtgggcggca acgggccggt tcagggcttc gtgaagcacg tggtgtacct
     2521 ggggaatctc ttcgaatacc gcgtgctcct cggagggcgg gagattcgcg tgcagcagga
     2581 ctcccatgaa gccttcaggg ccggagtgcc ggaggaaggg cgtccgtgcg cgctctcctt
     2641 caggaattta cgcttctacc aggaagacga agagggggta gcctcatgaa gatgttcggt
     2701 cgcagagagg gcaagctcga tctgaaccag attctcatgc tgatctgcct gacgattttg
     2761 atcatcacgg tggctatgcc catgatcatg attgtctaca acacgttttt ctatggtttt
     2821 acgttcgact ggagtatgtt tacgaatgtc gtcatggacc cctcgaatgt cgacgctatg
     2881 tggaacacga tcgtgatcgc gttctgggtc acggtggtcg ggaccatcgt cggggttttt
     2941 tacgcctggc tgatcggccg gacgaacatc cccctcaagg ggctgatgaa gacgctcttc
     3001 gtcatcccgt acatgttccc tcccttcatc ggggcgatgg cgtgggggct gctgctctct
     3061 ccgcgttcgg ggtacatcaa caagctgtgg atgctgatga tggggacgag agagcctctt
     3121 atgaacatca acagcatcgc cggaatcgtc ttcgtcgagg tctgctacta cttcccgttc
     3181 gttttcctcc aggtggtcag cgcgcttgag cgcatggacc cgacgctgga ggaatcggcc
     3241 cggatttccg gggcgaacca gctgtacgtg atccggaaga tcacgctgcc gctcgta
//
LOCUS       DAOTIL010000224         3295 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminobacteriaceae bacterium isolate AMR_MDS_3026
            NODE_34321_length_3295_cov_3.094136, whole genome shotgun sequence.
ACCESSION   DAOTIL010000224 DAOTIL010000000
VERSION     DAOTIL010000224.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868050
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminobacteriaceae bacterium (wastewater metagenome)
  ORGANISM  Aminobacteriaceae bacterium
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae.
REFERENCE   1  (bases 1 to 3295)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3295)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:19:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,224
            CDSs (total)                      :: 3,198
            Genes (coding)                    :: 3,184
            CDSs (with protein)               :: 3,184
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3295
                     /organism="Aminobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_34321_length_3295_cov_3.094136"
                     /isolate="AMR_MDS_3026"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:3034609"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1146)
                     /locus_tag="P5201_05790"
     CDS             complement(<1..1146)
                     /locus_tag="P5201_05790"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HRV98078.1"
                     /translation="MSMTEIVLNALLNLFALRAASAPSNLRGAVRKRVGFYLREHLGL
                     DAIGSYLELYDLTLDLQNEAPSELLLSRSAAVASGLRTQLPRDEQITILAMLLEAAPE
                     DAPSENEVLRAVAKAFAVERQFLVGYALFSGASFPWDGAGLDDRFLLPTDAPPQETRS
                     SPFRILRRPQFKRAFAVFRAEEEGPWFIRASSRGALFLDGIPFDGGIRRLRQGSVVSN
                     RQRNRIHFGEIAAAFSALSATARPHILEGDHLDFRYDGAPDTGLHDFSFSVRGGELVA
                     IMGGSGSGKSTLLSLLTGKLRPRSGRLLLDGRDVAGDAMLRRGILGYVPQDDLLFEDL
                     TVFENLYYSARLGLPDLPDAEVRRRCERMLDGLNQLDLLLHVGGRRFA"
     gene            complement(1238..2059)
                     /locus_tag="P5201_05795"
     CDS             complement(1238..2059)
                     /locus_tag="P5201_05795"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRV98079.1"
                     /translation="MKKAFYVLTVVIILALLSASGASAAEKSKEETLDAAYEHLVKVM
                     NPEEMKKLFALFDTMKDVGLSWNEDLLLPLRDIIACKTKEQLYVIWGMGSMNISYAML
                     FDKNPEGVWEGGREIDERIGFPRAELREESLAFAKSREGQASSSVDFIHAMLHRAKTD
                     QAVLNTLVGGFYGSTLELFYLFSSLGLASGVTEEFVQFVNAHLPQLTIAQEMLKAYSE
                     NGELAQLLDTGNRKAVIDSIVDIITKDMGKLTEDDLKQILSIVKAVRDPLAVPCN"
     gene            2367..3170
                     /locus_tag="P5201_05800"
     CDS             2367..3170
                     /locus_tag="P5201_05800"
                     /inference="COORDINATES: protein motif:HMM:NF013757.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IclR family transcriptional regulator"
                     /protein_id="HRV98080.1"
                     /translation="MMENSGIRVISRAFSLLVHLAQERRPLGISEIAEAVSLPKATVY
                     RILDSLESERAVMNRGGEYELGPVCLMLADAYRSQTGFAEAARPHLRALRDETKETVH
                     LFAYERGEFYYLDKLESPYQVRMHSRIGAKASLFRLSAGKALLARLSALEMNEIAEAV
                     PDEVAAEFPEILSRGFAVDNEQNERGLRCVGAAVFDRKGRPLGAVSVSAPVYRFPLEL
                     VESYGQLVKRTAEAIAGSADILFPAAAGGVARAGKSKGRLSSGTAPVEE"
BASE COUNT          654 a          953 c         1020 g          668 t
ORIGIN      
        1 ggcgaatctc cgaccaccca catggaggag aaggtcgagc tggttcaggc cgtcgagcat
       61 ccgttcgcag cggcgccgga cctctgcgtc cggaaggtcc ggcagtccga gacgcgcgct
      121 gtagtacaga ttttcgaaga cggtgagatc ctcgaagaga agatcgtcct gcggaacata
      181 ccccagaatg cctcgccgga gcatcgcgtc gcctgcgacg tcgcgtccgt cgagcagcag
      241 cctcccggag cgaggccgaa gcttgcccgt gagcagcgag agcagcgtcg attttccgga
      301 gccgctccct cccattatcg cgacaagctc gcctccccgc acggagaagc tgaagtcgtg
      361 aaggccggtg tcgggggcgc cgtcgtagcg gaagtccaga tgatctccct cgaggatgtg
      421 ggggcgggcc gttgcggaga gggcggagaa cgcggcggcg atctcgccga agtggatgcg
      481 gttccgttgc cggttcgaga cgaccgatcc ctgtctcagg cggcggatac cgccgtcgaa
      541 gggaatgccg tccagaaaca gtgctcccct ggaagaggcc ctgataaacc acggcccctc
      601 ttcctccgcg cggaagaccg cgaacgccct tttgaactgc ggacggcgga ggattcggaa
      661 cggagaggag cgcgtctcct gcgggggggc gtccgtaggg aggagaaacc tgtcgtcgag
      721 tccggccccg tcccacggga atgaagcgcc ggagaagagc gcgtagccga ccagaaattg
      781 tcgctccacg gcgaacgcct tggctacggc gcggagcacc tcgttttcgg aaggagcgtc
      841 ttcaggagcc gcttccagca gcatcgcgag gatcgtgatc tgttcgtcgc ggggaagctg
      901 ggttctcagc ccggaggcga ctgcggcgct tcgtgaaagc aagagctcgg aaggcgcttc
      961 gttctgcagg tcgagcgtca ggtcgtacag ctccaaatag gagcctatgg catcgagccc
     1021 gagatgttcg cgcagataga aacccacgcg tttccggacc gcgcccctga ggttcgaggg
     1081 agccgacgcg gcccgtaagg cgaagagatt cagcagcgcg ttcaagacga tttcggtcat
     1141 cgacatggct tagtctcctt ggccgaagtg aagagcagtg ttttcggcgt tgcccggttc
     1201 cgccggagcg tccgcgaagg acgccccggc gcgtggatca gttgcagggg acggccagag
     1261 gatcgcggac ggctttgacg atagaaagaa tctgcttcag gtcatcctcc gtcagcttcc
     1321 ccatatcctt cgtgatgatg tccacgatgg agtcgatgac ggcctttcga tttcccgtgt
     1381 cgagcagctg cgccaactct ccgttctccg aataagcctt gagcatctcc tgcgcgatgg
     1441 tcagctgcgg aaggtgggcg ttgacgaact gaacgaactc ctccgtgact ccggaagcga
     1501 ggccgaggct ggagaagaga tagaaaagct ccagcgtgct gccgtagaag cctcccacaa
     1561 gggtgttgag cacggcctga tcggtcttcg ccctgtgcag catggcgtgg atgaagtcga
     1621 ccgaactgct cgcctgcccc tcgcggcttt ttgcgaacgc gaggctttct tctcggagtt
     1681 ccgccctggg gaagccgatt cgttcgtcga tttcccttcc gccttcccaa actccttccg
     1741 gatttttgtc gaagagcatc gcgtagctga tgttcattga acccataccc cagataacat
     1801 agagctgttc tttggtttta caggcgatga tatcccggag cggaagaaga agatcctcgt
     1861 tccacgaaag accgacgtcc ttcatggtgt cgaagagcgc gaagagcttc ttcatctctt
     1921 ccggattcat taccttgacc agatgctcgt acgcggcgtc gagagtctct tccttgctct
     1981 tttcggcagc cgatgctccg gaggcactca ggagcgcgag gatgatcaca accgtcagta
     2041 cataaaaagc ttttttcata ccagtctctc cttcccttgt ctttctttat gctgtccgaa
     2101 attgttcact acaccatgtg tgcttttcgc cgtcctgttt cccgctccct tcatttttat
     2161 cgtttccggg gattccgttc ctcgttgaga acgagtatga catggaaacc cctcattttc
     2221 aaggaaacgc caaatccaac atattgcagg taaagagtat gagagtatgc ctcgtggcgc
     2281 gcgtgcaacg acggctcttg acattatgca tactgcgtcc tatgatgaat ctacactacg
     2341 gataaataat ccatggagcg aaatgaatga tggaaaacag cggcatcaga gttatttctc
     2401 gtgcattttc actccttgtg catctggcgc aggagcggcg ccctctcggc atctcggaga
     2461 tcgcggaggc ggtttcgctt cccaaggcca cagtctacag aatcctggac agccttgaaa
     2521 gcgaacgcgc agtgatgaac cggggcggcg agtacgaact tggccccgtc tgtctgatgc
     2581 tggccgacgc gtaccgcagt cagaccgggt tcgccgaggc ggcgcgtccg cacctccgcg
     2641 cgctgcggga cgagacgaag gagacggtcc acctcttcgc ctacgagcgg ggtgaatttt
     2701 actaccttga taagctcgaa agcccctatc aggtgcgtat gcactcccgc atcggggcga
     2761 aggcgtcgct tttccgtctc tcggcgggga aggcgcttct ggcgcgcctc tctgccctcg
     2821 aaatgaacga gattgcagag gcggttccgg acgaggttgc ggccgagttt cccgagatcc
     2881 tttcgagggg gttcgccgtg gacaacgagc agaacgaacg ggggctgcgc tgcgtcggcg
     2941 cggcggtctt cgacaggaag ggacgtccgt tgggagcggt gagcgtctcg gctcccgtct
     3001 accgttttcc cctggaactg gtcgaaagct atggacaact tgtgaagcgg acggccgagg
     3061 cgatcgccgg gagcgcggat attctcttcc ctgcggccgc cggcggcgtt gcgcgtgcag
     3121 ggaagagtaa agggcgtcta tcgtccggca cggcgccggt cgaggaatga tctaccagag
     3181 aacgtttttc tacagaagga ggaaaaagag atgatcaaaa caaacaagct cgtcatgatt
     3241 cccggtccca ccccggtggt ccgctccatc caggaccaga tggcccggga ggcgg
//
LOCUS       DAOTIL010000225         3295 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminobacteriaceae bacterium isolate AMR_MDS_3026
            NODE_34323_length_3295_cov_2.941667, whole genome shotgun sequence.
ACCESSION   DAOTIL010000225 DAOTIL010000000
VERSION     DAOTIL010000225.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868050
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminobacteriaceae bacterium (wastewater metagenome)
  ORGANISM  Aminobacteriaceae bacterium
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae.
REFERENCE   1  (bases 1 to 3295)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3295)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:19:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,224
            CDSs (total)                      :: 3,198
            Genes (coding)                    :: 3,184
            CDSs (with protein)               :: 3,184
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3295
                     /organism="Aminobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_34323_length_3295_cov_2.941667"
                     /isolate="AMR_MDS_3026"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:3034609"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(112..591)
                     /locus_tag="P5201_05805"
     CDS             complement(112..591)
                     /locus_tag="P5201_05805"
                     /inference="COORDINATES: protein motif:HMM:NF024304.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase family
                     protein"
                     /protein_id="HRV98081.1"
                     /translation="MGHPMRRNDREITDPAEIDGILEKARFGHLGVCDGDEPYVLPVN
                     FGVSEGAIYFHCAPEGRKLDVLRRNPKACFQVETDTELVASTTACGWGMLYRSVVVSG
                     VVSVVDSLEEKARGLTSLMKKCAGDSFSHVFSEQELGAVVVLRLDAVSRTGKARRPA"
     gene            742..2148
                     /locus_tag="P5201_05810"
     CDS             742..2148
                     /locus_tag="P5201_05810"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRV98082.1"
                     /translation="MKLVFERWRDNPQVQETTEKFSAAASRFLSVRGSSETDDRIAAR
                     KYWSELSSMYWTVAVALIDAQTESFPEELVFDEQERLFLDLGVVDQELTPFHPEITRL
                     LNSRAPAGLFQYYSFSDHIAECYSMVMAKPVTPPRSGYSIEEKIVQLERQLDSLKTRT
                     KVILPVVLSKVGVTSTETEMILSDLHRCLEAYTEVQMRTRKYREAEEKERQQMAVQNH
                     AFIEAEKRIAGALKGNEEDEGLDENEIAKVTKLLESAKTLARNIVYVEQELVKWDRKV
                     KKKAADLTAEAPAFRRRELRNMIEAKKEYISLTAKSARLDDSQLCQSDKLPFSIDKAA
                     AILEDMIALDPEMLVVARVRMYGIPRVILVPGQGYGTYDWNDHTLLIPAFPTYSQERA
                     AAYALGTFRWDSDEDRVIKNSYELIKENRDKSILDLNTSFYKDYFLWLTKEKKGYRIL
                     PRSTHKVFVQMFAPQKQE"
     gene            2173..2445
                     /locus_tag="P5201_05815"
     CDS             2173..2445
                     /locus_tag="P5201_05815"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRV98083.1"
                     /translation="MFERIISILLGSAILYLLRFKLLRKPLSPIRSILVATVIMVAGI
                     QFHYAYLPYETFQGGVTYTSLGFIAFLMFQYGYRKTAPSKKESAEK"
     gene            2486..3289
                     /locus_tag="P5201_05820"
     CDS             2486..3289
                     /locus_tag="P5201_05820"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF025054.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HRV98084.1"
                     /translation="MERDEAMSASAVAENFSATLRLWGALPTSRAEARGPCLFVSTGT
                     AIPDQNYAYCPVEEDAEENVRSSARFFREERMPFTWWFPPGTRGERFRRALEEQRMPE
                     RCAPPAMLMALDAPAERAHHSAERTVCKAVSSAEAEEWACSSLEGFDSGPEHQGPFSA
                     FCTSLLVPPFDEAFRLLTLRVGGRAAATALLSLTGEVAGIYYFSTLPAFRRRGLGRLL
                     LSEALEEARRSGCRTAVLQASPMGFALYKKAGFAVCGRFAVHSADPDAC"
BASE COUNT          713 a         1005 c          961 g          616 t
ORIGIN      
        1 cgggaaactg ctcccgcagg atctcctgaa cacgcgcaat cgcccttacg ttcgccgggg
       61 agcgtgcgtc gaaaaccctc ctgatgcgga acatcctccc gtcgccccgt atcaggccgg
      121 acggcgggct ttccccgtcc gggagaccgc atcgagccgg agcacgacga cagccccaag
      181 ctcctgctcc gaaaacacat gggagaagga gtctcccgcg cacttcttca tcagcgaggt
      241 caagcctcgc gccttctctt cgagagagtc gacgacggag acgacgccgg aaaccacgac
      301 gcttcggtag agcatgcccc aaccgcatgc cgtggtggag gcgacgagtt cggtatcggt
      361 ttccacttga aaacaggcct tcgggttgcg ccgcagcacg tcgagcttcc gcccttccgg
      421 ggcgcagtga aagtagatcg ccccttcgga gacgccgaag ttgaccggaa ggacgtacgg
      481 ttcgtcgccg tcgcacaccc caagatgccc gaaacgggcc ttctccaaaa tgccgtcgat
      541 ctccgcagga tcggtgattt ccctgtcgtt tcgccgcatc ggatgtccca tcgtttttcc
      601 tccttttgaa gtatcatctg ttccggatga atatgtgcgc tgtggaatat ggtatcataa
      661 acacggtatt ccgcacgtca gaatcgcatg caacgtgaag gaagtccggt gcacgcacgg
      721 agaatcggag gatgataccg aatgaagctt gtgtttgaac gatggaggga caacccgcag
      781 gtacaggaga ccacggaaaa gttctccgca gccgccagcc gttttttgtc cgtacgcggc
      841 tcctccgaga ccgacgaccg gatcgcagcc agaaaatact ggtcggaact ttcctcgatg
      901 tactggacgg tggctgtcgc tctcatcgac gcacagacgg aatcattccc cgaagaactt
      961 gtctttgacg agcaggaacg gctctttctc gatcttggcg tcgtggacca ggagctcacc
     1021 ccctttcatc ccgagatcac ccgcctgctg aactcaaggg cgcccgcggg gctctttcag
     1081 tactactcgt tctccgacca cattgcggag tgctacagca tggtcatggc caaacctgtc
     1141 accccgccgc gaagcggcta ctccatcgag gagaagattg tacagctcga aaggcagctc
     1201 gactccctca aaacaaggac gaaggtcatc cttcccgtgg tgctctccaa agtcggcgtc
     1261 acatcgacgg agacggagat gatactctcc gatctccacc gctgtcttga agcgtatacc
     1321 gaggtacaga tgcgcaccag gaagtacagg gaggccgagg agaaggagcg ccagcagatg
     1381 gccgtgcaga atcacgcttt catcgaagcc gagaagcgca tcgcgggcgc cctgaaaggg
     1441 aacgaggaag atgaggggct tgacgaaaac gagatcgcca aggtcacgaa gctgcttgag
     1501 agcgcgaaga cgctcgcccg gaacattgtc tacgtggaac aggaactcgt taaatgggac
     1561 cggaaagtca aaaagaaggc ggccgatctc acggcggaag ccccggcgtt ccggcgcagg
     1621 gaactccgga acatgatcga ggcgaagaaa gagtacatct cgcttacggc gaagtccgcc
     1681 cggctggatg actcacagct ctgccagagc gacaagcttc ctttttccat cgacaaggcc
     1741 gcggcaatcc tggaggatat gatcgcgctc gacccggaga tgctcgtcgt ggcacgagtt
     1801 cgcatgtacg gcataccgcg cgtgatcctc gtccccgggc aggggtacgg cacgtacgac
     1861 tggaacgacc acaccctgct gatcccggcg tttccgacct acagccagga gcgcgccgca
     1921 gcctacgccc tgggaacgtt ccgctgggac agcgacgagg accgcgtcat caagaacagc
     1981 tacgagctca tcaaggagaa ccgcgacaag tccatcctcg acctgaacac gtcgttctac
     2041 aaggactatt tcctctggct gacgaaagag aagaagggct accgcatcct gccgcgcagc
     2101 acgcacaagg tcttcgtaca gatgttcgcg cctcagaaac aggaatgaaa aacagagtaa
     2161 aaggagagtc caatgtttga acgaatcatt agtatacttc ttggcagcgc catcctctat
     2221 cttctgcgct tcaagctgct gcggaaaccg ctctcgccga ttcgatccat tctcgtcgcg
     2281 acggtcatca tggtcgccgg aatacaattt cactacgcct atctccccta cgagacgttt
     2341 caagggggcg tcacctacac gtcgcttggc ttcattgcgt tcctgatgtt ccagtacggc
     2401 taccgcaaaa ccgctccctc caagaaagag agcgcagaaa agtagcccgt ccacgctgca
     2461 gacttcataa aaaggcggag cggcaatgga gagagacgaa gcgatgagtg catctgctgt
     2521 tgcggaaaac ttcagcgcga cgctgcgact ctggggggcg ctgccgacgt cgagagccga
     2581 ggcgcgcggc ccctgtctct tcgtgtcgac cggaacggcc ataccggacc agaactacgc
     2641 gtactgcccc gtggaagagg acgccgagga gaacgtacgg agttcagcgc gtttttttcg
     2701 cgaggagagg atgcccttca cgtggtggtt cccccccgga acacggggag aacgcttccg
     2761 gagagcgctt gaggagcagc gaatgcccga gcggtgcgcg ccgccggcga tgctcatggc
     2821 gctcgacgca ccggcggaac gcgcacacca ttctgcggag cgcaccgttt gcaaggctgt
     2881 ctcatccgct gaggccgagg aatgggcctg ttcctccctt gaaggattcg actcggggcc
     2941 ggagcatcaa ggacctttct ccgcattctg cacgtcgctt ctcgtaccgc ctttcgacga
     3001 agcgttcaga ttgctgacgc ttcgcgtggg ggggcgcgct gcggcaaccg cccttctctc
     3061 tctgaccgga gaggtggcag gaatctacta cttctccact ctccccgcct tccgcaggcg
     3121 agggctgggg aggctcctgc tgtcggaagc gctggaggag gcgcggcgct ccggctgtcg
     3181 tacagccgtg ctccaggcct cccccatggg atttgcgctg tataaaaaag ctggtttcgc
     3241 cgtgtgcggc cgttttgcgg tccactccgc cgatcccgac gcctgttaag tcgtt
//
LOCUS       DAOTIL010000226         3284 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminobacteriaceae bacterium isolate AMR_MDS_3026
            NODE_34469_length_3284_cov_2.885413, whole genome shotgun sequence.
ACCESSION   DAOTIL010000226 DAOTIL010000000
VERSION     DAOTIL010000226.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868050
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminobacteriaceae bacterium (wastewater metagenome)
  ORGANISM  Aminobacteriaceae bacterium
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae.
REFERENCE   1  (bases 1 to 3284)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3284)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:19:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,224
            CDSs (total)                      :: 3,198
            Genes (coding)                    :: 3,184
            CDSs (with protein)               :: 3,184
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3284
                     /organism="Aminobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_34469_length_3284_cov_2.885413"
                     /isolate="AMR_MDS_3026"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:3034609"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..778)
                     /locus_tag="P5201_05825"
     CDS             complement(<1..778)
                     /locus_tag="P5201_05825"
                     /EC_number="2.8.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF013323.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008410 - CoA-transferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-transferase"
                     /protein_id="HRV98085.1"
                     /translation="MSKVKSAIEAVGLIRDGATVAIGGFVGCMHPEELSNAVEERFLQ
                     SGTPNDLTLVYAAGQGDRKDRGANHFGHPKLLKRVVGGHWGLAPKLQALALENKIEAY
                     NFPQGVISHLFRDIAAKKPGTITHIGLKTFVDPRLEGGKLNARTTEDLVEIVEIGGTE
                     YLRYMPFPIDVALVRGTFCDAAGNATFEKEALALEVVAMCQAAKNSGGIVVLQVEKVL
                     PKSELNAKLVRLPGIYVDAIVEATPERHMQTFGVQYNPSYC"
     gene            1257..2171
                     /locus_tag="P5201_05830"
     CDS             1257..2171
                     /locus_tag="P5201_05830"
                     /inference="COORDINATES: protein motif:HMM:NF015431.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HRV98086.1"
                     /translation="MDIQHLKYFVQVIKNQSFTKAAEKLVVTQPMLSRVINQLEEELD
                     VKLIERTSKLFKTTDAGELLYQQALKVLHTHNDIYQYINDLKSAKSGEVLISIPGVLL
                     DMYFPPLLFEFHQKYPNVKLDIIEEGSKLTAQSVLSEQVELGFVMLPVRNASKLTSRI
                     VTTSICHVLVNKEHRFATRNTPIHTSELVEERIITFSTTATLHDAFIRHCENEGFEPN
                     IVYKTLMPNFIFEMVHLGLCIGILPYPVVKRFIFDGLVSVPLEPKIIWEIAVISKKGN
                     YLSFAARNFRSFVEEYSLKNEKEWQGLE"
     gene            complement(2184..3080)
                     /locus_tag="P5201_05835"
     CDS             complement(2184..3080)
                     /locus_tag="P5201_05835"
                     /inference="COORDINATES: protein motif:HMM:NF012245.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HRV98087.1"
                     /translation="MKGKENKKDENGWSEKQRSLPEGRPVDHYIYVLSRLSGMQVADV
                     GVCICDLDGVVFYRPARSLDLKVKHGDEIRPGSLLYRALHERRRVTAKMDSSLYGVPY
                     VGVGTPIMDEGGQVLGAVAITESTLNYENLKESSMKIAESIEMLAGASEEIAAQSEEM
                     SGMAAIMKETLESSRESVKASDQVIGLIKMIATRTNLLGLNASIEAARVGEQGRGFSV
                     VAEEIRKLAGVTGDSIKNIAHTLASIQSDNAEICRQMLVMSSIITQVSDATTQIAETA
                     QALNEMASSLNEMAEKMMDSAN"
BASE COUNT          805 a          940 c          664 g          875 t
ORIGIN      
        1 cgcaatagct ggggttgtac tgaacgccga acgtttgcat gtgcctctcc ggcgtggctt
       61 ctacgatcgc gtcgacataa attccaggga gacggaccag cttggcgttg agttcgctct
      121 tcggaagcac cttctccacc tgcagaacga cgatacctcc gctgttcttc gcggcttggc
      181 acatggccac gacttcgagc gccagcgcct ctttttcgaa cgtcgcgttt ccggccgcgt
      241 cgcagaacgt accccgtacc agggctacat ctattggaaa cggcatgtac cgcaaatatt
      301 ccgtaccgcc tatttcgacg atttccacca ggtcttccgt tgttctcgca ttcaatttgc
      361 cgccctcaag acgagggtca acgaatgttt tcaacccgat gtgcgtaatc gttccgggct
      421 ttttcgccgc gatgtccctg aacagatgcg atatgactcc ctgcgggaag ttgtacgctt
      481 cgatcttgtt ttccagagcc agagcctgca gcttcggagc aagcccccaa tgccctccta
      541 cgactcgttt gaggagcttc ggatgtccga agtgattcgc tcctctgtcc ttcctgtctc
      601 cctgcccggc ggcgtacaca agggtcagat cattcggcgt tccgctttgt agaaaacgtt
      661 cctccactgc gttggaaagc tcctcgggat gcatgcaccc gacgaaccct cctatcgcca
      721 ctgtcgctcc gtccctgatc aaaccaaccg cctcgatcgc cgatttcact ttgctcatac
      781 ccgcaacacg ctccttccga actctttacc cctctcaccc gtccttgaga gatcgttttc
      841 attcctctgg gacaaggctt tctcaagctc ccaacacaaa gcatatttca atattaaata
      901 caatgcgcac aaccaaaaga actttttttc acaaataacg caatagccga ataggctcag
      961 ttcccactca cagcttcata gaaaaatccg gaaaaagctc gagagatgct ttttcggatg
     1021 gcggaacaca caggtgaaat gaaagcgcta catagcaacc ggttttcaaa acagcttacg
     1081 caaaaactct ttaaaataaa tgctttttta attttgcatc ttgagcattt atttttttca
     1141 tgctacaatt ctatagtcta aactaagtct atgtaaaata ctttctttta aatggttcaa
     1201 tgcctacatg taatgtatta tatagtggct acttacccaa aagattggag catacaatgg
     1261 acattcaaca tttaaaatac tttgttcaag ttataaagaa ccaaagtttc accaaggccg
     1321 ccgagaagct tgtggtcaca caaccaatgc tttcaagagt catcaaccag ctcgaagaag
     1381 aactcgacgt caaactgata gaacgaacca gcaaactctt caaaacgacg gacgccggag
     1441 aactcttgta tcagcaggca ttgaaagtac tgcacaccca caacgacatc tatcagtaca
     1501 tcaacgatct taaatccgca aaatccggcg aagtattgat cagcatcccc ggcgtcctcc
     1561 tggacatgta cttcccccct cttctcttcg agttccatca aaaatacccg aatgtcaagc
     1621 tcgacattat cgaagagggc tccaagctta cggcgcaatc agtcttgtcc gagcaggttg
     1681 aactggggtt cgtcatgctc cctgttcgca acgcctctaa gttgacaagc cgtattgtca
     1741 ccacgagcat ctgccatgtc cttgtaaata aagagcaccg tttcgccaca agaaacactc
     1801 cgatccacac gtcggaactt gtcgaagagc gcatcataac cttcagtacg acggcaaccc
     1861 tgcacgacgc gtttatacga cactgcgaga acgaaggctt cgagcccaat atcgtatata
     1921 aaacgctcat gcccaatttc attttcgaga tggtgcacct cggcctttgc atagggatac
     1981 ttccataccc tgttgtcaaa cgattcattt tcgacgggct cgtctcggta ccgctggaac
     2041 cgaaaattat ctgggaaata gcggtcatca gtaaaaaggg gaattatctc tcgtttgccg
     2101 cacgaaattt ccgttcattc gtcgaagaat attctctgaa aaatgaaaaa gagtggcagg
     2161 gactcgaatg aaacgcgcca gccttagttt gccgaatcca tcattttttc cgccatttcg
     2221 ttcagagaac tcgccatctc gttcaacgct tgcgcggtct cggcgatctg cgtcgtcgca
     2281 tcggacactt gagtaattat cgaagacatg acgagcatct gccggcagat ttccgcattg
     2341 tcggactgaa tggatgccaa cgtgtgcgct atatttttta tcgagtcgcc cgtaacgccg
     2401 gcaagttttc tgatctcttc cgcgacaacg ctaaagcctc ttccctgttc gccaaccctc
     2461 gccgcctcta tggatgcgtt caaccctaaa agattcgttc gcgtcgctat catcttgata
     2521 agccctatta cctgatcgct tgccttcacg ctttctctgg agctttcaag agtctccttc
     2581 attatcgcgg ccataccgct catttcctcc gattgcgcgg caatctcctc ggaagcccct
     2641 gccagcatct caatgctttc cgctatcttc atagaagatt ctttgaggtt ttcatagtta
     2701 agggtcgatt ccgttattgc aacagctccc aaaacttgac cgccttcgtc catgataggg
     2761 gtgcctaccc ctacgtacgg caccccgtaa agagatgagt ccatcttcgc cgttactctc
     2821 cgccgctcat gcagagctcg atacaagaga cttccggggc gaatctcgtc gccatgtttg
     2881 accttcaaat ccaaggagcg cgccggcctg tagaaaacca cgccgtcaag atcacagata
     2941 cagacgccga catccgcaac ctgcattccg ctcaaccggg acagaacgta tatatagtga
     3001 tcaactggcc tcccttcggg cagactgcgc tgcttttcgc tccatccatt ttcatccttc
     3061 ttattttctt tacccttcat atgctgcaac tctctcttca tattccaaag tgcatttcag
     3121 ctattcttcc cgtttgaatg aatatgtttc agcatcttga aaaacatctt atcccttcgc
     3181 cttatggttt cttcatcctt accgttggag tagtcgtaaa atccgctctt ggtttttact
     3241 cccaactgct gtgtctccat caagtttttc aatgtaaccg aagg
//
LOCUS       DAOTIL010000227         3268 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminobacteriaceae bacterium isolate AMR_MDS_3026
            NODE_34698_length_3268_cov_3.904139, whole genome shotgun sequence.
ACCESSION   DAOTIL010000227 DAOTIL010000000
VERSION     DAOTIL010000227.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868050
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminobacteriaceae bacterium (wastewater metagenome)
  ORGANISM  Aminobacteriaceae bacterium
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae.
REFERENCE   1  (bases 1 to 3268)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3268)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:19:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,224
            CDSs (total)                      :: 3,198
            Genes (coding)                    :: 3,184
            CDSs (with protein)               :: 3,184
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3268
                     /organism="Aminobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_34698_length_3268_cov_3.904139"
                     /isolate="AMR_MDS_3026"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:3034609"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..908)
                     /locus_tag="P5201_05840"
     CDS             complement(<1..908)
                     /locus_tag="P5201_05840"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HRV98088.1"
                     /translation="MSEIRIENVVKRFGDFVAVNDVSLVVAEHEFMVLLGPSGCGKTT
                     LLRAIAGLGMADEGRISIGGRDVTYLPPRERRISMVFQSYAIFPHMNVYDNIAFGLRM
                     QKEEKTEIENRVRRAAELLHIEAMLDRYPSKMSGGQRQRVAVARAIAMQAQVLLMDEP
                     LSNLDALLRLEMRAELKQLLAEIGTTTVYVTHDQIEAMSMGDRIAVMHGGTVQQVDSP
                     TTVYDYPANRFIGGFIGNPPMNFLEGQVRRKDGDTFVRLGEFDVTPTPRMQPVLKEYD
                     GKPIVLGIRAENLETLYEPAHDALRT"
     gene            complement(934..1797)
                     /locus_tag="P5201_05845"
     CDS             complement(934..1797)
                     /locus_tag="P5201_05845"
                     /inference="COORDINATES: protein motif:HMM:NF012738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HRV98089.1"
                     /translation="MSVRVSDEMTKVRARAKRRLVIKRALTYLTAIVLSLWILLPIWL
                     IASMALTTPEAMRNFPKGVLPFIPFSLDTMRFFLEARGIIPGLINSVVVALITLALST
                     LIAAPAGYALSRYVFRGRNAFRLSILAVRAFPVVILSIPLAVTFINLGMFDKVSSLAL
                     MHTALTLPTTVLVIGSVFASIPYELEEAAQVFGCTAFQAFRRVVLPLVLPGIAAAALF
                     TFVMSWNEVFAATVLTVQNRTLPAQVLVSLREAPDAYKFAGGFFMLIPSVIFIMIIRK
                     YLFNMWGQSSK"
     gene            complement(1794..3188)
                     /locus_tag="P5201_05850"
     CDS             complement(1794..3188)
                     /locus_tag="P5201_05850"
                     /inference="COORDINATES: protein motif:HMM:NF012738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HRV98090.1"
                     /translation="MSIQPLGTPRRRTKVQWAPYLLITPALLYLLVFFAWPMTQAIRL
                     AVWDEQGLLTLRAAPGELNKAGALEQGAQITVIDRRNVPLAPGEADGGPALLDVWFFV
                     EGETPGGATVQGWAPESRVRVRETDPSGVPLRGTVRPRLGNTAGQHTTLHASPDERSD
                     VVGELASRAEIGIAQRTSLEVWFLIEGVRNEQTLSGWAPSRRLQIYGDGATGRVDSGD
                     TGRFTLLHIQRMLNDRFFRSALGTTFLLMLLIIPVQLVLAIVMALVIQARLKFNLLFL
                     AIFAIPLAASDLAVGIVWSSIFTQNGYLNSILYSLGLIESPQAYLTAATRHWILVAVW
                     IAEVWRATSLVMVIVVSGLQAISQEVLEAAELFGATLWQRVRYVILPLLKPSLQVALI
                     LRTILALQVFAVVIALGGGDVVTVLANETYRQYYALRNMNVAAAYSGLILLLSMISAG
                     IYLRTVRGQEEAVS"
BASE COUNT          629 a         1013 c         1049 g          577 t
ORIGIN      
        1 tcggtccgca gtgcgtcatg ggccggttcg tacagcgtct ccagattctc ggccctgatg
       61 cccagcacga tgggcttgcc gtcgtactcc ttcaggaccg gctgcattcg gggcgttggc
      121 gtcacgtcga actctcccag gcggacaaaa gtatcgccgt ccttcctgcg gacctgccct
      181 tcgagaaagt tcatcggcgg gttcccgatg aatcctccga taaagcggtt cgcagggtag
      241 tcgtacacgg tcgtcgggga gtccacctgc tggaccgttc cgccgtgcat caccgcgatg
      301 cggtcgccca tgctcattgc ctctatctgg tcgtgcgtga cgtagacggt ggtcgttccg
      361 atctcggcca gaagctgttt cagctcggcg cgcatctcca gccgcagcag cgcgtcgaga
      421 ttgctcaacg gctcgtccat cagcagcacc tgcgcctgca tcgcgatggc gcgcgccacc
      481 gccacgcgct gcctctgacc gccgctcatc ttcgacgggt agcggtcgag catcgcctcg
      541 atgtgcagca gctccgccgc ccgcctcacc cggttttcta tctccgtctt ctcctccttc
      601 tgcatccgca ggccaaaggc gatattgtcg tagacgttca tatgcgggaa gatggcgtag
      661 ctctgaaaga ccatcgagat gcgccgttcc cgcgggggca ggtaggtgac gtcccgcccg
      721 ccgatgctga tcctgccttc gtccgccatg ccgagcccgg cgatggcgcg gagcagcgtc
      781 gtcttgccgc agccgctggg acccagcagc accatgaact catgctcggc gacgacgagc
      841 gagacgtcgt tcaccgccac gaagtcgccg aacctcttga cgacattttc tattcgtatc
      901 tccgacatga ttccgctcct tccgtaattt tgttcacttg ctggactgcc cccacatgtt
      961 gaaaaggtac ttgcggatga tcatgatgaa gatcaccgag ggaatcaaca tgaagaatcc
     1021 tcccgcgaac ttgtacgcgt cgggggcctc ccgcagcgag acgagcacct gcgcgggcag
     1081 cgtgcgattc tgcaccgtga gcactgtggc cgcgaagact tcgttccagg acatcacgaa
     1141 ggtgaagagc gccgcggcgg cgatgccggg cagcaccagc ggcagcacga cccgccggaa
     1201 tgcctggaag gccgtgcagc cgaagacctg tgccgcctcc tcgagttcat acggaatgct
     1261 cgcgaagacg cttccgatga cgagcaccgt cgtgggcagc gtgagcgcag tatgcatcag
     1321 cgcaagactt gagaccttgt cgaacattcc caggttgatg aaggtcaccg caagcggaat
     1381 ggagaggatg accaccggaa aggcgcgcac ggcgaggatg gagaggcgga atgcgttacg
     1441 gcctcggaag acgtagcgcg agagcgcgta tcctgccggc gccgcgatca gcgtggagag
     1501 ggccagggtg atcagcgcga cgacgacgct gttgataaga ccggggatga taccgcgcgc
     1561 ctccaggaag aagcgcatgg tgtccaagga gaacggaatg aagggcagca cgcccttggg
     1621 aaagtttcgc atcgcttccg gcgtggtgag ggccatcgac gcgatgagcc aaatcggaag
     1681 aagaatccac agagagagaa caatcgccgt cagataggtg agcgcccgct tgatcacgag
     1741 gcggcgcttc gcgcgagcgc gaaccttcgt catctcgtcc gaaacccgta cgctcatgag
     1801 accgcctcct cctgtccgcg tacagtacgc agatagatac cggcgctgat catcgaaagc
     1861 aggagaatca ggcctgaata tgcggcggcc acgttcatgt tgcggagggc gtagtactgg
     1921 cggtaggtct cgttggcgag caccgtgacc acgtcgcctc cgccgagcgc gatcaccacg
     1981 gcgaagacct gcagcgcgag gatggttctc aggatgaggg cgacctgaag gcttggcttg
     2041 agcagcggaa gaatcacgta gcgcacccgc tgccacaggg tcgcgccgaa gagttcggct
     2101 gcctccagca cctcctgcga gatcgcctgc agcccggaga cgacgatcac catcacgagg
     2161 gacgtcgccc gccagacctc ggcgatccag accgcgacca ggatccagtg gcgcgtcgcg
     2221 gcggtgaggt atgcctgcgg gctttcgatc agcccgaggg agtagagaat gctgttcaga
     2281 tagccgttct gcgtgaagat ggaggaccag acgatcccca cggcgaggtc ggacgcggcc
     2341 agcgggatgg cgaagatggc gagaaagagc agattgaact tcagccgcgc ctgaatcacc
     2401 agcgccatca cgatcgccag cacgagctgc acagggatga tcagcagcat caacaggaag
     2461 gttgttccca gcgcggagcg gaagaagcgg tcgttgagca tcctctgaat gtggagcagg
     2521 gtgaagcggc cggtatcgcc ggagtccacc cttccggtcg cgccgtcgcc gtagatctgc
     2581 agcctgcggg agggcgccca tccggagagc gtctgttcat tgcgtactcc ttcaataaga
     2641 aaccagacct caagagatgt ccgttgcgca ataccgatct ccgctctgct cgcaagctcc
     2701 ccgacgacgt cgctccgttc gtcgggggaa gcgtgcagcg tcgtgtgctg tccggcggtg
     2761 tttccgaggc gggggcgaac cgtgccccgg agcgggacgc ccgagggatc agtttcgcgg
     2821 acgcggacgc ggctttcggg cgcccaccct tggaccgtgg ctccgccggg ggtctcgccc
     2881 tcaacaaaaa accagacgtc gaggagggcg gggcctccgt ccgcttcgcc gggggcaaga
     2941 gggacgttcc ggcggtcgat cacggttatc tgcgccccct gttcgagcgc gcccgcctta
     3001 ttcagctctc ccggcgccgc gcgcagcgtg aggagaccct gttcgtccca gaccgccagg
     3061 cgaattgcct gcgtcatcgg ccaggcgaag aagaccagca gatagagcag ggcgggcgta
     3121 atcaagaggt acggcgccca ctgcactttc gtgcggcgcc ttggtgttcc gagaggctgt
     3181 atgctcatgt ttttcatctc ccttcagcgg aagacgcgga gtatccgcgc ttttgtgaac
     3241 gtgcggatac ccgctgtgaa cggggaga
//
LOCUS       DAOTIL010000228         3252 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminobacteriaceae bacterium isolate AMR_MDS_3026
            NODE_34915_length_3252_cov_3.312480, whole genome shotgun sequence.
ACCESSION   DAOTIL010000228 DAOTIL010000000
VERSION     DAOTIL010000228.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868050
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminobacteriaceae bacterium (wastewater metagenome)
  ORGANISM  Aminobacteriaceae bacterium
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae.
REFERENCE   1  (bases 1 to 3252)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3252)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:19:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,224
            CDSs (total)                      :: 3,198
            Genes (coding)                    :: 3,184
            CDSs (with protein)               :: 3,184
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3252
                     /organism="Aminobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_34915_length_3252_cov_3.312480"
                     /isolate="AMR_MDS_3026"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:3034609"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2684)
                     /gene="ygfK"
                     /locus_tag="P5201_05855"
     CDS             complement(<1..2684)
                     /gene="ygfK"
                     /locus_tag="P5201_05855"
                     /inference="COORDINATES: protein motif:HMM:TIGR03315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative selenate reductase subunit YgfK"
                     /protein_id="HRV98091.1"
                     /translation="MSDIMRMIPFGNLMDWMLVEHGKEGSIFGIRKNKFYKNATGKQI
                     VVCGESISSPIGPAAGPNSQLAQNIIAAYLAGSRFIELKTVQIMDGEELRKAVAKPCI
                     NAQDEGYNVEWSTELTVQEAFDEYVKAWFAIQVMAKELGISAQRDFAYNMSVGYDLKG
                     IQSPKIDGFIEGLKNASGSGIWKTCSQWLRDNLSKFSNVNASDVDAIESRLCSSITLS
                     TLHGCPPEEIERISRYLFEEKGLHVFVKCNPTLLGYETARDLLDRMGYTYIDFTDHHF
                     KHDLQYSDGVAMFKRLQEFAKERGLEFGLKLTNTFPVNIKNEELPGEQMYMSGRSLFP
                     LTITLASKLSREFGGKLQISYSGGADFFNIDSILNVGIQPISMATTILKPGGYERMKQ
                     IAEKAEPYLNGAFRGVDVKALDALAASVFEKRFHKKSARPVGSRKTESRLPLFDCAKA
                     PCQDGGCPINQQVPTYLRLVNEKKYAEAFDVIAMDNSSPAVTGTICDHQCQHVCTRLD
                     YEQPLEIREMKKIAVLNAQDEYIARMQPAKIVSKKKAVVVGAGPAGVGTAFFLRRNGI
                     ETTVLERRAEPYGIVKYVIPEFRIGEDMIARDVALAKKAGVQFVFGVDGKIDIEALKK
                     EYDYVVLATGAWKPGESPVKEGRELVRDALSFLEEYKKKKGHVEIGGTVAIIGGGDVA
                     MDCARAAKRAPGVKRSIVVYRRTVAYMPAEPEEKELALHDGVELMELLAPVKYDGKTL
                     LCEKMQLGEKDASGRRGVTGTGEMVPVAADTVIGATGAKVDSEIFGANGIALDAKGRV
                     VVNEENETTVSNVYVAGDCKAGPATVVKGIADAKTVTKSILTKEGLSPDFAVTELVQD
                     LASLYEKKGVLKEAVKDQNDGARCLACDVVCELCCDVCP"
     gene            complement(2721..>3252)
                     /locus_tag="P5201_05860"
     CDS             complement(2721..>3252)
                     /locus_tag="P5201_05860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009200607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HRV98092.1"
                     /translation="DIACFAMYSHDQFQIQASASFTGSESPPPFMAQFAEVDVDTETG
                     KVRVVKFVSVGDCGQPLNPKLVEGQIEGATMNGISYALTEQYIFNSKGKMTNNSFWDY
                     KLFGTLDMPEMKTIVVDSDEPTGPYGAKSISEIGINGPAPAIANAIYDAVGVRIYDLP
                     LTPEKVLKAIRARTDG"
BASE COUNT          637 a          992 c          860 g          763 t
ORIGIN      
        1 gggcagacgt cgcagcagag ttcgcagacg acatcgcatg cgaggcagcg ggcgccgtcg
       61 ttctgatctt tcacggcttc tttcaagacg ccttttttct cgtacaacga cgccagatcc
      121 tgcacaagct ccgtgaccgc gaaatcgggg gagagcccct cctttgtaag aatgctcttc
      181 gtcacggttt tcgcgtcggc tatccccttg accaccgttg ccggtcctgc cttgcagtca
      241 cctgcgacgt agacgttcga aacggtggtt tcgttttctt cgttcactac aacgcgcccc
      301 ttggcgtcga gcgcgatgcc gttggctccg aagatttctg agtccacctt cgcgccggtc
      361 gcgccgatga ccgtgtccgc cgccaccggg accatttcgc ccgtaccggt cacgccgcgc
      421 cgccccgagg cgtccttctc gccgagctgc atcttttcgc agaggagcgt cttcccgtcg
      481 tacttcacag gggcaagcag ctccatgagc tccaccccgt cgtgcagcgc cagttccttc
      541 tcttccggtt cggcaggcat ataggcaacg gtgcggcggt agacgacgat cgaccgcttc
      601 actccggggg cgcgcttcgc cgccctggcg cagtccattg cgacatcgcc gccgccgatg
      661 atcgcgacag tgccgccgat ctcgacatgc cccttcttct ttttatactc ttcaaggaag
      721 gaaagcgcat cgcgaaccag ctcccgcccc tctttcaccg gagactcgcc gggtttccaa
      781 gctcccgtcg caagcacgac atagtcgtac tccttcttca gcgcttctat atctattttt
      841 ccgtcgaccc cgaagacgaa ctgtactcca gccttcttcg cgagagcgac gtcgcgggca
      901 atcatgtcct cgcctatacg gaactctgga atgacatact tcacgatgcc gtacggttcg
      961 gcccggcgct caagcaccgt cgtctcgatg ccgttccgcc gaaggaaaaa cgctgtcccc
     1021 actccggcag gaccggcgcc gacaacaacc gcctttttct tcgagacgat ttttgcaggt
     1081 tgcatccggg caatatactc atcttgcgcg ttgagcacgg caatcttctt catctcgcgt
     1141 atctcaagcg gctgttcgta gtcgagccgg gtacagacgt gctggcactg atggtcgcag
     1201 attgttcccg tgactgccgg cgaactgttg tccatcgcga tgacatcgaa cgcttcggcg
     1261 tacttcttct cgttgaccag acggagatac gtcggaacct gctggttaat ggggcacccg
     1321 ccgtcctggc agggggcctt ggcgcagtcg aaaagcggaa gacgcgattc ggtcttcctt
     1381 gatccaacag ggcgggcgct ttttttgtgg aagcgctttt caaagacgct cgccgcgagg
     1441 gcgtcaagag ccttcacgtc cactccgcgg aacgccccgt tcaggtacgg ctccgctttc
     1501 tcggctatct gcttcattct ttcgtatccg ccgggcttga gaattgtagt ggccatgctg
     1561 atcggctgaa taccgacatt cagtatggag tcgatattaa agaagtcggc gccgcctgag
     1621 tacgagatct ggagctttcc gccgaattcg cggctcagct tgctcgccaa ggtaatcgtc
     1681 agggggaaaa gcgaacggcc ggacatgtac atctgttcac cgggcagctc ttcattttta
     1741 atattcacgg ggaaggtgtt ggtaagcttc agaccgaact cgagtccccg ctccttcgcg
     1801 aactcctgaa gcctcttgaa catcgcaacg ccgtcgctgt actgaaggtc gtgcttgaag
     1861 tgatggtccg tgaagtcaat ataggtatac cccatccggt caagaagatc tcgggccgtt
     1921 tcatacccca gaagcgtcgg attgcacttc acaaagacgt ggagcccctt ctcctcgaac
     1981 agataccgtg aaatacgttc gatctcctcc ggcgggcagc cgtgaagcgt agaaagcgtt
     2041 atcgacgaac ataaccggct ttcgatagca tccacatccg aagcattgac gttgctgaac
     2101 ttcgaaaggt tgtcccgcag ccactgggaa cacgtcttcc agatgccgct cccggaggcg
     2161 ttcttcaacc cttcgatgaa cccgtcgatc ttgggggact gaattccctt caaatcgtag
     2221 ccgacgctca tattgtaggc gaaatcgcgt tgtgcgctga ttccaagctc ctttgccatc
     2281 acctggattg cgaaccaggc tttgacgtat tcgtcgaacg cttcctggac agtcagctcc
     2341 gtagaccatt ccacgttgta cccttcgtcc tgagcgttga tacagggctt ggctaccgcc
     2401 ttccgaagtt cttctccgtc catgatctgc acggttttca gctcgataaa gcgtgaaccg
     2461 gccagatagg ctgcaatgat attctgcgca agctgggagt taggaccggc tgcagggccg
     2521 atcggcgagc tgatggattc gccgcagacc acgatctgct ttcccgtagc gttcttatag
     2581 aatttgtttt tacggatacc gaagatggag ccttccttgc cgtgttcgac cagcatccag
     2641 tccatcaggt ttccgaaagg aatcattctc ataatgtcgc tcattcctgc acctccgcaa
     2701 tcataatcgc tggattttgc ttacccatcc gtacgcgcgc gaatcgcctt gagcaccttc
     2761 tcgggcgtca ggggaagatc gtaaatccgg acgccgacgg cgtcataaat cgcgttcgcg
     2821 atagccggag ccgggccgtt gataccgatt tcagaaatgg acttcgcccc gtacggcccc
     2881 gtcggctcgt cggaatcgac cacaatggtc ttcatctcag gcatatccag cgtgccgaac
     2941 agtttgtaat cccagaagga gttattggtc atcttcccct tcgagttgaa aatgtactgt
     3001 tcagtcagcg cgtagctgat accgttcatt gtggcgccct cgatctgccc ttcgacaagc
     3061 ttggggttga gcggctggcc gcagtctccc acagacacga acttgacaac gcgcaccttt
     3121 cctgtttcgg tatcgacgtc gacctcggcg aactgcgcca tgaaaggagg gggcgactcc
     3181 gagcctgtga acgacgccga agcctgaatc tggaactggt catgactgta catggcaaag
     3241 caggcaatat cg
//
LOCUS       DAOTIL010000229         3249 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminobacteriaceae bacterium isolate AMR_MDS_3026
            NODE_34962_length_3249_cov_3.870382, whole genome shotgun sequence.
ACCESSION   DAOTIL010000229 DAOTIL010000000
VERSION     DAOTIL010000229.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868050
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminobacteriaceae bacterium (wastewater metagenome)
  ORGANISM  Aminobacteriaceae bacterium
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae.
REFERENCE   1  (bases 1 to 3249)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3249)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:19:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,224
            CDSs (total)                      :: 3,198
            Genes (coding)                    :: 3,184
            CDSs (with protein)               :: 3,184
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3249
                     /organism="Aminobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_34962_length_3249_cov_3.870382"
                     /isolate="AMR_MDS_3026"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:3034609"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..419
                     /locus_tag="P5201_05865"
     CDS             <1..419
                     /locus_tag="P5201_05865"
                     /inference="COORDINATES: protein motif:HMM:NF018513.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="TRAP transporter large permease subunit"
                     /protein_id="HRV98093.1"
                     /translation="ILAAEQIPDKIAKAVFALSENRLVILFLMNALMLFMGTFLDISP
                     IIIILVPILFPIARQLGVTPIHFGIMTIVNMAIGQCTPPVGISLFVATGIAKAKLGEI
                     LGAYMKYLLTMIVVLAIITIFPQIITFLPHMVMGLK"
     gene            581..1561
                     /locus_tag="P5201_05870"
     CDS             581..1561
                     /locus_tag="P5201_05870"
                     /inference="COORDINATES: protein motif:HMM:NF015445.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter substrate-binding protein"
                     /protein_id="HRV98094.1"
                     /translation="MSKKLFALALLVMFTVILTASAASAAVVLKLSNVLPDGNPTTES
                     LVFFAEKVKEKTGGEVEVRLFNNSALGGQREALESMQAGTLEMCMANAGPMAQFVPTM
                     DVLSLPYVFQSTEHMFKALNGKAGEMIKADIDKAGFVFLYWGDGGSRNLINNKKPITK
                     PEDLKGLKIRVMDSRLMVNTLNAMGAIATPMAQGEVYSALQQGVLDGWENNPVTLLTL
                     KLYEVSDYFSWTQHFMVPDLVLISKQAFEKLTPEHQKALLEAGRESEAKQREYWNAFV
                     GKTVEELKSHNVKFNDVDIAPFVQSVQPVWEDYTKKFGTELLDLITSAAK"
     gene            1637..2305
                     /locus_tag="P5201_05875"
     CDS             1637..2305
                     /locus_tag="P5201_05875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005660677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RraA family protein"
                     /protein_id="HRV98095.1"
                     /translation="MARWTTDKELFSLMKNELFTALVGDVLDTMGLTKQFLPPRIRPL
                     LPEMTLAGRAMTVLEADCCGAGRKPFGLMFEALDDLKEDEVYICAGGSPDFAHWGGLM
                     TIRAKHLNAAGAVTHGYTRDTRQVLELDFPVFSWGTYAQDQGARGRVIDWRCPVEFVN
                     GVRVETGDLLFGDMGGVVVVPRAVESEVIEKALEKAEGENLVAKAIMGGMSTVEAFAT
                     FGIM"
     gene            2337..>3249
                     /locus_tag="P5201_05880"
     CDS             2337..>3249
                     /locus_tag="P5201_05880"
                     /inference="COORDINATES: protein motif:HMM:NF012335.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding alcohol dehydrogenase family
                     protein"
                     /protein_id="HRV98096.1"
                     /translation="MRSAKLVAPRQIEIFEDRMPLPGEGEVLVRVKAAGICGSDLHIY
                     QGERADVTLPRILGHELVGEVESSGGGADRFSKGDRVTIDPVVSCGSCVSCKRGYDNL
                     CSTVKCLGVQVDGGYCDFIVVPEEKVYALPSSLPWEKAALIEPFSIAAEVLSRSEVRE
                     GDKVLVLGAGTIGLCILQAMKLAGAEVLTSDFVESRLEKAKELGADRVVNSKTGDLSA
                     ALREFTGEFGADVVVEAVGIPRLLEESLIYAAPGGRIVVLGFNPEPAKIPEVLVVKKE
                     LKIIGSRMNCRKFPQVIEWFEKGLVNPE"
BASE COUNT          640 a          862 c         1113 g          634 t
ORIGIN      
        1 ggatacttgc cgcggagcag atccccgaca agatcgcgaa ggcggtcttc gccctctcgg
       61 agaaccggct ggtcatcctc tttctgatga acgcgctgat gctcttcatg gggaccttcc
      121 tcgacatctc cccgatcatc atcatcctcg tccccatcct cttcccgata gcccgtcagc
      181 tcggcgtgac gccgattcac ttcggcatca tgaccatcgt gaacatggcg atcggacagt
      241 gtacgcctcc tgtgggcatc tctctcttcg tggcgacggg catcgcgaag gcgaagctgg
      301 gggagatcct gggggcgtac atgaagtacc tgcttaccat gatcgtcgtg cttgcgatca
      361 tcaccatctt cccgcagatc attacgttcc tcccgcatat ggtgatggga ttgaagtaat
      421 tctgcgacgg cggggcttct cgcccggccg gacatggaac gaagggcggt gggaacaggc
      481 ggcggagttt gctaagaaca gcgcgaacag gattcacccc tcggcggcga cgagggaaag
      541 aacagcagtc catcagctga tacccgaagg aggtagcatt atgtcgaaaa agttgtttgc
      601 tcttgcgctc cttgtgatgt tcacggttat tctgactgca tccgcggcgt ccgccgcagt
      661 tgttctgaag ctgtccaacg ttcttcccga cggcaatccg acgacggagt ccctggtttt
      721 cttcgccgag aaggtgaagg agaagacggg cggcgaggtg gaagtgcgcc tcttcaacaa
      781 cagcgcgctc ggcggtcagc gcgaggcgct tgaaagcatg caggcgggca cgctcgagat
      841 gtgcatggcc aacgccgggc cgatggccca gttcgttccc acgatggacg tcctctccct
      901 cccctacgtc ttccagagca cggagcatat gttcaaggcg ctcaacggga aggccggaga
      961 gatgatcaag gcggacatcg acaaggccgg attcgtcttc ctctactggg gcgacggcgg
     1021 ttcgcgcaac ctgatcaaca acaagaagcc aatcacgaag cccgaagact tgaaggggct
     1081 gaagatccgc gtcatggaca gccgcctgat ggtgaacacg ctcaacgcga tgggcgccat
     1141 cgccacgccg atggcgcagg gcgaagtgta cagcgcgctg cagcagggag tgctcgacgg
     1201 atgggagaac aaccccgtca cgctgcttac gctgaagctc tatgaggtgt ccgactactt
     1261 ctcgtggacg cagcacttca tggtgcccga ccttgtgctc atcagcaaac aggcgttcga
     1321 aaaactgact cccgaacatc aaaaagcgct gttggaggcc gggcgcgagt ccgaggccaa
     1381 gcagcgggag tactggaacg cgttcgtcgg caagacggtg gaggagctga agtcgcacaa
     1441 cgtgaagttc aacgatgtgg atatcgctcc cttcgtccag tccgtgcagc cggtgtggga
     1501 ggactatacg aagaagttcg gaacggaact gctcgacctt attacgtcgg ctgcgaagta
     1561 acgggacgtt cttttttccg gaggagggcg gcgcgccgcg ctccttcctc cgttcaacga
     1621 aaggcgggag cagggcatgg cgagatggac aacggataaa gagctgttct ctctgatgaa
     1681 aaatgagctg tttacagcgc ttgtgggcga tgtgctggac acgatggggc tgacgaagca
     1741 gtttcttccg ccgcggatac ggccgcttct gccggagatg acgcttgccg gacgggcgat
     1801 gacggtgctg gaggcggact gctgcggggc gggccggaag cccttcgggt taatgttcga
     1861 ggccctcgac gatctgaagg aagacgaggt gtacatctgc gcgggtggct cgccggactt
     1921 cgcgcactgg ggcggtctga tgaccatccg ggcgaaacat ctgaatgcgg ccggagcggt
     1981 gacgcacggc tatacgcgcg atacgcggca ggtgctggag cttgattttc cggtgttctc
     2041 gtggggaacg tacgctcagg atcagggggc gcgcggacgc gtgatcgatt ggcgctgtcc
     2101 ggtcgagttc gtcaacggcg ttcgcgtgga gacgggagac cttctcttcg gcgatatggg
     2161 cggcgtcgtg gtggttccgc gcgccgtgga gagcgaagtc atcgagaagg cgctggagaa
     2221 ggccgaggga gaaaatttgg tggcgaaggc gatcatgggc ggcatgtcca ccgtggaggc
     2281 ctttgcgacc tttggaataa tgtagattct ggcccgaaag ggcggaggtg aatgctgtga
     2341 gaagtgcaaa gctcgttgca cccaggcaga ttgaaatttt tgaggacagg atgccccttc
     2401 ccggcgaggg agaggtgctc gtccgtgtga aggctgccgg gatctgcggt tccgacctgc
     2461 atatctacca gggggagagg gcggacgtga cgctcccccg gattcttggc cacgagctgg
     2521 tgggcgaggt agagtcctcc gggggcggcg cggaccgctt ctccaagggg gacagggtga
     2581 cgatcgaccc tgtggtttcg tgcggaagct gcgtttcgtg caaacggggg tacgacaacc
     2641 tgtgcagcac ggtcaagtgt ttgggggtgc aggtcgacgg cgggtattgc gatttcatcg
     2701 tcgtgccgga ggagaaggtg tacgcgctcc cgtcttctct gccgtgggag aaggcggcgc
     2761 tgatagagcc cttctcgatc gcggcggagg tgctttcacg aagcgaagtc cgtgaagggg
     2821 acaaggtgct cgtccttgga gccgggacaa tcggcctgtg cattctgcag gcgatgaagc
     2881 tcgccggagc cgaggtgttg acgagcgatt tcgtggagag ccggctggag aaggcgaagg
     2941 aactaggcgc cgacagggtt gtgaacagca agaccggcga tctttcagcg gcgctgcggg
     3001 agttcaccgg agagttcggc gccgatgtgg tggtcgaggc cgtgggcata ccccggcttc
     3061 tggaggagag ccttatctac gctgctccgg gagggcggat cgtggtgctc ggcttcaacc
     3121 cggagcctgc gaagatcccc gaggtgctcg ttgtgaagaa agagctgaag atcatcgggt
     3181 cgcgcatgaa ctgccggaag tttcctcagg tgatcgagtg gttcgagaag gggttggtga
     3241 acccggagc
//
LOCUS       DAOTIL010000230         3245 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminobacteriaceae bacterium isolate AMR_MDS_3026
            NODE_35028_length_3245_cov_3.916614, whole genome shotgun sequence.
ACCESSION   DAOTIL010000230 DAOTIL010000000
VERSION     DAOTIL010000230.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868050
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminobacteriaceae bacterium (wastewater metagenome)
  ORGANISM  Aminobacteriaceae bacterium
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae.
REFERENCE   1  (bases 1 to 3245)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3245)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:19:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,224
            CDSs (total)                      :: 3,198
            Genes (coding)                    :: 3,184
            CDSs (with protein)               :: 3,184
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3245
                     /organism="Aminobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_35028_length_3245_cov_3.916614"
                     /isolate="AMR_MDS_3026"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:3034609"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..584
                     /locus_tag="P5201_05885"
     CDS             <1..584
                     /locus_tag="P5201_05885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013047515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003852 - 2-isopropylmalate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009098 - leucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="alpha-isopropylmalate synthase regulatory
                     domain-containing protein"
                     /protein_id="HRV98097.1"
                     /translation="GKHSGKHAFKQRVEEMGFTLSDEKLKEAMALFKTLCDRKEIVTA
                     DDLEAMINNEILSIDESRRITLKNFLVQVGSGHGSATVTIEDEGVEKTDAAAGNGPVD
                     AAYRAIRRIVGIEPELENYAIQAVNESSDAIGEARVTLDLGGVRVNGRGASTDVIEAS
                     IKAYIDGINRLFAAQARIAGQEAQAAEEARKAV"
     gene            664..1953
                     /locus_tag="P5201_05890"
     CDS             664..1953
                     /locus_tag="P5201_05890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013047514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydratase large subunit"
                     /protein_id="HRV98098.1"
                     /translation="MGRTLASAIIAAHSIEGAREGEICKVNVDFAFANDITAPPAIVS
                     FKEMGAEKVFDKERCAVLPDHFTPNKDIASAEQTKKGREFALSQGMLYWEVGRVGIEH
                     AFLPEKGYILPGDIVIGADSHTCTGGALGALSTGVGSTDLAAVWALGETWLKVPPTIR
                     VNYEGKRPAWVSGKDMILSLIGRIGVQGARYMALEFGGDGVEALPMDDRFTIANMAIE
                     AGGKAGLFVPDAKMLAYANARAARKFSPVYPDADAAYFRTETFDMEKMVPVAAMPHSP
                     DNVKPVRECGSPEIHQVFIGACTNGRIRDIAAAAKMLEGKQVAAGVRCIVIPASYEVY
                     NQALDMGYLRIFTEAGAVVCTPTCGPCLGGHMGILAAGERCVSTSNRNFVGRMGSTKS
                     ELILASPLTAAASAITGRLTDPRDVASPDFFKKLEEE"
     gene            1956..2468
                     /locus_tag="P5201_05895"
     CDS             1956..2468
                     /locus_tag="P5201_05895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014162310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydratase small subunit"
                     /protein_id="HRV98099.1"
                     /translation="MSKIFKGNAWKYGDNIDTDVIIPARHLVTSDEAELGKHCMEDID
                     KDFVKKVAPGDMIVGGENFGCGSSREHAPIAIKGAGCSCVIAASYARIFFRNAINVGL
                     PIFECPEAAASIETGDEIEVDPGAGTIVNKTKGKTFTVAPFSPFLQDLIQQGGLIPYV
                     SKELGRRKGA"
     gene            2465..>3245
                     /locus_tag="P5201_05900"
     CDS             2465..>3245
                     /locus_tag="P5201_05900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013047512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isocitrate/isopropylmalate family dehydrogenase"
                     /protein_id="HRV98100.1"
                     /translation="MSGRKYTIARVPGDGIGPEVIGETSKILEAAGSKHGFSISWADY
                     PFGADHYLKTNEILPASAVDEMAPMDALLLGAVGDPRVKPGILERGILLHLRFHFDQY
                     VNLRPAKSYPRVPLPIQPKKGPTLNTLVVRENTEDFYVGIGGKTEKGKTVVDLATTRG
                     LYSMSGTLEGAFSDGVEGAFQLGVASEPGVRRVVAYAARAAKARGEEKITLASKSNAL
                     PQIYGFWEDVAKDEAAKQGVGLSIVNVDAMCYHLVRTPDLYG"
BASE COUNT          661 a         1041 c         1055 g          488 t
ORIGIN      
        1 tgggcaagca ctccggaaag cacgccttca agcagcgcgt ggaagagatg ggcttcaccc
       61 tctccgacga gaagctgaag gaggccatgg cgctcttcaa gacgctctgc gaccggaagg
      121 aaatcgtcac agccgacgac cttgaggcca tgatcaacaa cgagatcctc tcgatcgacg
      181 agagccgccg catcacgctg aagaacttcc tcgtgcaggt cggcagcggg cacggcagcg
      241 cgaccgtcac tatagaggac gagggcgttg aaaaaaccga cgcggctgcg ggcaacggcc
      301 cggtggacgc ggcttacagg gcgatcagac gtatcgtcgg catcgagccg gagctggaga
      361 actatgcaat ccaggccgtc aacgaatcct ccgacgccat cggcgaggcg agagtgactc
      421 tcgacctcgg cggcgtcagg gtaaacggca ggggcgccag cacggacgtc atcgaagcga
      481 gcatcaaggc gtacatcgac ggtatcaacc gtcttttcgc ggcgcaggcg cgcatcgcgg
      541 ggcaggaggc gcaggcggcc gaagaggcgc ggaaggcggt atagttttcc ccccgctcac
      601 gcagaagcgc ggcggcgggg gcatcgaaac cgggtatcat tggaaaggag tgcgtgtgta
      661 cacatgggaa gaacattggc aagcgccatc atcgccgccc actcgatcga gggggcaagg
      721 gagggcgaaa tctgcaaggt gaacgtcgac ttcgccttcg cgaacgacat cacggctcct
      781 ccggccatcg tctccttcaa ggagatggga gcggagaagg ttttcgacaa ggagcggtgc
      841 gcggtactcc ccgaccactt cacgccgaac aaggacatcg cctctgcgga acagaccaag
      901 aaggggcgcg agttcgccct ctcgcagggg atgctctact gggaggtcgg ccgcgtgggc
      961 atcgaacacg cgttcctccc cgagaagggg tacattctcc ccggcgacat cgtcatcggc
     1021 gcggacagcc acacctgcac aggcggcgcg ctgggcgctc tctccacggg cgttggcagc
     1081 accgacctcg ccgccgtctg ggcgctgggc gagacgtggc tgaaggttcc ccccaccatc
     1141 cgggtgaact atgaggggaa gcgccccgca tgggtgtcgg gcaaggacat gatcctctcg
     1201 ctcatcggcc gcatcggcgt acagggcgca cgctacatgg cgctcgagtt cggcggcgac
     1261 ggagtggaag cgcttccaat ggacgaccgc ttcaccatcg ccaacatggc gattgaggcg
     1321 ggcggcaaag ccggactctt cgtgcccgac gcgaagatgc ttgcgtacgc gaacgcgcgg
     1381 gcagcccgaa agttctctcc ggtctacccc gacgcggatg cggcgtactt ccgtaccgag
     1441 acgttcgaca tggagaagat ggttccagta gctgccatgc cgcactctcc cgataatgtc
     1501 aagccggtcc gcgaatgcgg ctctccggag atccaccagg tcttcatcgg cgcatgcacc
     1561 aacggccgca tccgcgacat cgcggccgcc gcgaagatgc tcgaaggaaa acaggtggcg
     1621 gccggcgtgc gctgcatcgt catccccgcc tcgtacgagg tctacaacca ggcgctcgat
     1681 atggggtacc tgcgcatctt cacggaggcg ggcgcggttg tctgcactcc gacgtgcggc
     1741 ccctgcctcg gcggccacat gggcattctc gccgcgggcg agcgctgcgt ctccacaagc
     1801 aaccggaact ttgtggggcg catgggaagc acgaagagcg aactcatcct ggcgagcccg
     1861 ctgacggctg cggccagcgc catcacgggc aggctcacgg atccccgcga cgtagcctcc
     1921 cccgacttct tcaagaaact ggaggaagag tagccatgtc caagatattc aaaggaaacg
     1981 cgtggaaata cggcgacaat atcgacaccg acgtcatcat cccggcccgt catctggtca
     2041 ccagcgacga ggcggagctg ggcaagcact gcatggagga catcgacaag gacttcgtga
     2101 agaaggtcgc gccgggcgac atgatcgtgg gcggcgagaa cttcggctgc ggctcttcgc
     2161 gcgaacacgc gcccatcgcc atcaagggcg cggggtgcag ctgcgtcatc gcggcctcgt
     2221 acgcgcgcat cttcttccgc aacgccatca acgtcggcct gccgatcttc gagtgcccgg
     2281 aggccgcggc ctccatcgag accggggacg aaatcgaagt cgaccccggc gcggggacca
     2341 tcgtgaacaa gaccaagggg aagaccttca ccgtcgcccc cttctccccc ttcctccagg
     2401 atctcatcca gcagggcggc ctcatcccct acgtcagcaa ggagcttgga cggaggaagg
     2461 gcgcatgagc ggcaggaagt acaccatagc ccgcgtcccc ggggacggca tcggccccga
     2521 ggtcatcggc gagacgtcca agatccttga agcagcaggc tcgaaacacg gtttctccat
     2581 ctcgtgggcc gactacccct tcggcgcgga ccactacctg aagacgaacg agatcctccc
     2641 cgcgtcggcg gtggacgaga tggcgccgat ggacgcgctg ctgctgggag cggtcggcga
     2701 tccgcgcgta aaaccgggaa ttcttgaacg cggcattcta ctgcaccttc gtttccactt
     2761 cgaccagtac gtgaacctcc ggccggcgaa gagctacccg cgggttcccc tgcccatcca
     2821 accgaagaag gggccgaccc tgaacaccct cgtcgtacgt gagaacacgg aagacttcta
     2881 cgtcggcatc ggcggcaaaa cggaaaaggg gaagaccgtg gtcgatcttg cgacgacgcg
     2941 agggctctac tccatgagcg gaacgctcga aggcgcgttc tcggacggcg tggagggcgc
     3001 gttccagctc ggcgtggcct cggaaccggg cgtccgccgc gtggtcgcgt acgccgcgcg
     3061 cgccgcgaag gcccgaggcg aagagaagat cacgctggcc tcgaaatcga acgccctgcc
     3121 gcagatctac ggcttctggg aggacgtcgc gaaggacgag gcggcgaaac agggcgtcgg
     3181 gctctccatc gtgaacgtgg acgccatgtg ctaccacctg gtgcgcaccc ctgacctcta
     3241 cggcg
//
LOCUS       DAOTIL010000231         3241 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminobacteriaceae bacterium isolate AMR_MDS_3026
            NODE_35083_length_3241_cov_3.177024, whole genome shotgun sequence.
ACCESSION   DAOTIL010000231 DAOTIL010000000
VERSION     DAOTIL010000231.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868050
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminobacteriaceae bacterium (wastewater metagenome)
  ORGANISM  Aminobacteriaceae bacterium
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae.
REFERENCE   1  (bases 1 to 3241)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3241)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:19:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,224
            CDSs (total)                      :: 3,198
            Genes (coding)                    :: 3,184
            CDSs (with protein)               :: 3,184
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3241
                     /organism="Aminobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_35083_length_3241_cov_3.177024"
                     /isolate="AMR_MDS_3026"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:3034609"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..770
                     /locus_tag="P5201_05905"
     CDS             <1..770
                     /locus_tag="P5201_05905"
                     /inference="COORDINATES: protein motif:HMM:NF014684.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HRV98101.1"
                     /translation="AIAAAWVGIYAPPLPKVIVLFLVCAAGFLAGGAWATIPALLKTK
                     YRVDDVVTTLLLNYVMWHLMGYLLFGPLQMPGSSWPRSPAVAEIARFPVLLIRSRFHL
                     GIVIALVAVLVVWFVNSKTVFGYQSRAVGVNPRAAAFGGINVNAVIMKTALLSGGLAG
                     LAGVGEVVAIHFHLLMDISPGFGYSGIVIAMLGRLHPVGTALSAFFFSVIIVGAQSMS
                     RLTGVPTYIAEVIQGMALIVMLIALLLTEYRVKAVRD"
     gene            770..1714
                     /locus_tag="P5201_05910"
     CDS             770..1714
                     /locus_tag="P5201_05910"
                     /inference="COORDINATES: protein motif:HMM:NF014684.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HRV98102.1"
                     /translation="MEQVMTTAFITGLLAAMMRMATPIIFGTLGEILCERAGVLNLGI
                     EGIMLMGAMTGFLTTLTTGSLWLGVAAAALVGVLLSLFMAFLAVYLGLSQHVSGLGIT
                     LFSTGLAMFIYRLAVGSPVNPPTVRPFTQTAIPFLSGIPILGTALFNQYVLVYIALLL
                     VPLIWFLFYRTSWGLAIRTVGENPFAADTVGINVNLTRTLCLAAGGALMGVGGAFLTL
                     AHQNMFLIDVVGGRGWIAIAMVIFGNWDPAKGMAGALIFGFLDALQLRLQGLGFDIPF
                     HLFLIIPYLMTVIALVGVSRRAAAPAGLLKPYRREEKG"
     gene            1866..>3241
                     /locus_tag="P5201_05915"
     CDS             1866..>3241
                     /locus_tag="P5201_05915"
                     /inference="COORDINATES: protein motif:HMM:NF014758.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043546 - molybdopterin cofactor binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase family protein
                     molybdopterin-binding subunit"
                     /protein_id="HRV98103.1"
                     /translation="MTLQHVGKSVPRVDAVRKATGEAVFVADIVLPRMLHAKVLRAGV
                     PHAKILSIDTSKAEAMPGVKKVVTGQGCGMLFGTCLWDQPPLAVDKVRHAGEPVAVVL
                     AETGNQAAAALKEIAVEYKKLPFVLDPVEAAAPGAPLIHEKNGEYRRVEYSVHPIPGS
                     NVFHHYKLRKGNEQEGFAKADVTVEDEFEFPLSSQAPLEPHGAICRFGTDGDIEIWAS
                     NQAPFVLRDVLADMFGIPATKVRVHIPYLGGGFGGKSDVSIEPMVAYAASFVPGYAVK
                     LVLTRGEVISGALIGRGMKGRMRIGAKLDGTFTALKAEMYFADGAYGDTGWPVDTVAG
                     HNCTGPYEFPNAIVDVYGVYTNSPIVGAYRGYGHPEGQFMSSRLIDMLARKLDMTPAE
                     IMRKNFLCEGRKNALGQTIKKSNGDLFGCLDAVEKALAEEALPPNDERFVYGRGIASM
                     MKSPKMAA"
BASE COUNT          515 a         1067 c         1062 g          597 t
ORIGIN      
        1 gcgccatcgc cgccgcctgg gtcggcatct acgcgccccc ccttccgaag gtgatcgtcc
       61 tttttctcgt ctgcgccgca gggtttctcg ccggaggcgc gtgggcgacg atccccgcgc
      121 tgctgaagac gaagtacagg gtggacgacg tggtcaccac gctgctgctg aactacgtca
      181 tgtggcacct gatgggatac ctgctcttcg gtccgctgca gatgccgggg tcgagctggc
      241 cccgctcccc cgccgtggcg gagatcgcgc gcttcccggt gctgctgatc cgctcgcgct
      301 tccatctggg catcgtcatc gcgctcgtcg ccgtgctcgt cgtctggttc gtgaacagca
      361 agacggtctt cggctaccag tcgcgcgccg tcggggtgaa cccgagggcg gcggccttcg
      421 gcggcatcaa cgtgaacgcg gtcatcatga agaccgcgct cctctccggg gggctcgcgg
      481 ggctcgcggg cgtgggcgag gtggtcgcga tccacttcca cctgctgatg gacatctctc
      541 ccggcttcgg ctactccggc atcgtcatcg cgatgctcgg gcgactgcac ccggtcggaa
      601 cggcgctctc cgcgttcttc ttcagcgtga tcatcgtggg ggcgcagtcg atgagccgcc
      661 tgaccggcgt cccgacgtac atcgcggagg tgatccaggg gatggcgctc atcgtgatgc
      721 tcatcgccct gctgctgacc gaataccgcg taaaggcggt gagagactga tggaacaggt
      781 gatgaccacc gcttttatca cgggacttct ggcggccatg atgcgcatgg cgacgcccat
      841 catcttcggc acgcttgggg agatcctctg cgaacgcgcc ggggtgctca acctcggtat
      901 cgagggaatc atgctgatgg gggcgatgac gggctttctc actacgctga ccacgggctc
      961 gctctggctc ggcgtagcgg ccgcggcgct cgtcggcgtg ctgctctccc tcttcatggc
     1021 cttcctcgcc gtctacctgg ggctctcgca gcacgtctcg gggctcggga tcacgctctt
     1081 ctcgacaggg ctggccatgt tcatctacag gctcgcggtc ggctccccgg tgaacccacc
     1141 cacggtgcgc cccttcacgc agacggcgat tccgttcctc tcgggcattc ccatcctggg
     1201 gacggctctc ttcaaccagt acgttctggt ctatatcgcg ctgctcctcg tgccgctgat
     1261 ctggttcctg ttctaccgca cctcgtgggg gctcgccatc cgcaccgtcg gggaaaaccc
     1321 cttcgccgcg gacacggtag gcattaacgt caacctgacg cggacgctct gcctcgcggc
     1381 gggcggcgcg ctcatgggcg tcggcggcgc gttcctgacc ctggcccacc agaacatgtt
     1441 cctgatcgac gtggtgggcg gcagggggtg gatcgccatc gccatggtga tcttcggcaa
     1501 ctgggacccg gcgaagggga tggcgggcgc gttgatcttc ggcttcctcg acgcgctcca
     1561 gctgcgcctc caggggctgg gcttcgacat tccgttccac ctcttcctga tcatccccta
     1621 cctgatgacg gtcatcgcgc tcgtcggcgt ttcgcggcgc gcggcggctc cggcggggct
     1681 gctgaagccg taccggaggg aggagaaggg gtagaggaaa ctccggggag cacgggcgcg
     1741 ttccttctgc ccgtgcgtgt ccggcgtcac tacttccggg gggcggcgcc ctgcgcactt
     1801 ttcgggctcg ctccatttcg gagcaacttt ttcatcgcat ctctttgtga aagagagggg
     1861 aaagcatgac gttacagcat gtaggaaaga gcgttccgcg cgtggacgcg gtccggaagg
     1921 cgacgggaga ggccgtcttc gtggccgaca tcgttctgcc gcggatgctc cacgcgaagg
     1981 tgctgagggc cggggtccct cacgccaaaa ttctctcgat cgacacgtcg aaggcggagg
     2041 cgatgcccgg cgtcaagaaa gtcgtcacag ggcaggggtg cggaatgctc ttcggcacct
     2101 gcctctggga ccagccgccc ctggccgtcg acaaggtccg ccacgcgggc gagcccgtcg
     2161 cggtcgtcct tgccgagacc gggaatcagg cggccgcggc gctcaaggag atcgccgtcg
     2221 agtacaagaa gctcccgttc gtcctcgacc ctgtcgaagc ggcggctccc ggcgcgccgc
     2281 tcatccacga gaagaacgga gagtaccgga gagtggaata ctccgtccat ccgatccccg
     2341 gaagcaacgt cttccaccac tataagctcc gcaaggggaa cgaacaggag gggttcgcca
     2401 aagccgacgt cacggtcgag gacgagttcg agttccccct gtccagccag gcgccgctcg
     2461 agccgcacgg cgcgatctgc cgcttcggca cggacggcga tatcgagata tgggcctcca
     2521 accaggcccc gttcgtcctc cgggacgtcc tcgccgacat gttcggcatc cctgcgacaa
     2581 aggtgcgcgt gcacatcccc tacctcggcg gcggtttcgg cgggaagtcc gacgtcagca
     2641 tcgaaccgat ggtcgcctat gcggcgagct tcgtccccgg atatgccgtc aagctggtac
     2701 tcacgcgcgg cgaggtcatc tccggagcgc tgatcggccg cgggatgaag gggcgcatgc
     2761 ggatcggcgc gaagctcgac ggcacgttca ccgcgctgaa ggccgagatg tacttcgcgg
     2821 acggcgcgta cggcgacacg gggtggcccg tggacaccgt cgccggacac aactgcaccg
     2881 gcccttacga atttcccaat gcgattgtcg acgtctacgg cgtctacacc aattcgccga
     2941 tcgtcggcgc gtacagaggg tacggacacc ccgaggggca gttcatgtcc agccgcctga
     3001 tcgacatgct tgcgcggaag ctcgatatga cgccggcgga gatcatgcgg aagaacttcc
     3061 tctgcgaggg gcggaagaac gccctcggcc agacgatcaa gaagagcaac ggcgacctct
     3121 tcggttgcct cgacgccgtc gaaaaagctc tcgcggagga ggcgcttccc cccaacgacg
     3181 agcgtttcgt ctacggcagg ggaatcgcct cgatgatgaa gtcgcccaag atggcggcga
     3241 a
//
LOCUS       DAOTIL010000232         3238 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminobacteriaceae bacterium isolate AMR_MDS_3026
            NODE_35125_length_3238_cov_3.207980, whole genome shotgun sequence.
ACCESSION   DAOTIL010000232 DAOTIL010000000
VERSION     DAOTIL010000232.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868050
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminobacteriaceae bacterium (wastewater metagenome)
  ORGANISM  Aminobacteriaceae bacterium
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae.
REFERENCE   1  (bases 1 to 3238)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3238)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:19:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,224
            CDSs (total)                      :: 3,198
            Genes (coding)                    :: 3,184
            CDSs (with protein)               :: 3,184
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3238
                     /organism="Aminobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_35125_length_3238_cov_3.207980"
                     /isolate="AMR_MDS_3026"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:3034609"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            217..591
                     /locus_tag="P5201_05920"
     CDS             217..591
                     /locus_tag="P5201_05920"
                     /EC_number="2.7.7.-"
                     /inference="COORDINATES: protein motif:HMM:NF014021.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase domain-containing
                     protein"
                     /protein_id="HRV98104.1"
                     /translation="MMKRIEETRSSRTEDFMRTVDKAVNVILNSGLDVEKIYLFGSYA
                     YGEPNEESDIDLYVVLSDESKPRIRAIDAMEKIGMKIFKSDVYNVEVLAGFEGDFLAR
                     ASLPTMEKTVFDKGVTLYERTA"
     gene            575..1012
                     /locus_tag="P5201_05925"
     CDS             575..1012
                     /locus_tag="P5201_05925"
                     /inference="COORDINATES: protein motif:HMM:NF017020.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HEPN domain-containing protein"
                     /protein_id="HRV98105.1"
                     /translation="MSGPLDEEVAAQAREWMRLAEMDLEVANYLFGYHQIPVEIICYH
                     SQQSAEKYIKALMVSSGEEPPKTHNLIDLARICKEKKIAAEIWDGMASEIVGLNRFGT
                     VPRYPSKIELAESDARRAVEDASSIREVITGRIPSQTSCSGPI"
     gene            complement(1175..2509)
                     /locus_tag="P5201_05930"
     CDS             complement(1175..2509)
                     /locus_tag="P5201_05930"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRV98106.1"
                     /translation="MVNKLFVQPLLFASPVYWPDRAELDAAGKAVAGMLEAMDAPTSL
                     MRTARIVAGDADLDDALSSLGRAVPLLVPLSGGVQKRMLAAAGAASESLLWSFFPNDD
                     IYPEGERPTITRIVSKNALPAVADVWGTLRNRGCRVAKVYHEGSWLAAARELKAAHRV
                     RSARLLLVGYTQEWVVSASVDTRKMERLLGISSVHIGLEELFAEFETCRNDSVSASLA
                     KKFADGARSCLEPNMEQIEQAFRMYRALKNLLARYGCNALAISCFSLAKTLGVTACVA
                     LSMLNDEPETVAACEGDLDSAVSMIVGKAITGMPVFMGNPVYNLENTLDIVHCTAPRK
                     LLSANAQPYEIRSHHETGLSVSQRVEVEGERDATIFRIGNEFRNAVLFEAPLLSNPRL
                     DTCRTQFRFGLKSWEACFDAMLGCHHMVAFGRHGDALERFMKQRLGLEVVRF"
     gene            complement(2548..>3238)
                     /locus_tag="P5201_05935"
     CDS             complement(2548..>3238)
                     /locus_tag="P5201_05935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009165355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HRV98107.1"
                     /translation="ALLGVSLEVKRGSIVSIIGSNGAGKSTLLNTISGLVKQERGKIF
                     LTEGIGPDTAKLLPREPYRVVKLGVVQVPEGRQVFPGLTVRENLMAGAYTVNDAGRVR
                     RNLDRMFEMYPILKERARQQAGTLSGGEQQMLAICRGLMAEPKVLLLDEPSLGLAPII
                     VKGVFDTVRRIRDEGITVVLVEQNANQALAIADYAYVLENGRVALEGEGRALLCDPAV
                     KDAYLGGAQEA"
BASE COUNT          622 a         1021 c         1022 g          573 t
ORIGIN      
        1 gttctccgcg acgcacccgg agttcagcct cgaagagtac cggagcatac tcgaagagtg
       61 cggcgtcgaa tggggcttcg acgaaatgcg ggacgccgac gaggcgcccg ccccgtgatg
      121 ctctttccgt ccgggagacg atcttctgat acagccatcg tgatatactg tgaagcgatc
      181 gggtactatc ggactccgca tcaataagga gtgaccatga tgaagcggat cgaagaaacg
      241 agatcgagtc gcaccgaaga ctttatgagg actgtcgaca aggccgtcaa cgtcatcttg
      301 aacagcggac tcgacgtcga aaaaatctat ctgttcggtt cctacgccta cggagagcct
      361 aacgaggagt cggacatcga tttgtacgtc gtgctctcgg acgagagcaa gccccgcatc
      421 agggcaatcg acgcgatgga aaagattgga atgaagattt tcaagtccga cgtgtacaac
      481 gtggaggtgt tggcgggatt cgaaggggat tttctcgcca gggcttcttt gccgacgatg
      541 gagaagactg ttttcgacaa gggagtgacg ctgtatgagc ggaccgcttg acgaggaagt
      601 cgcggcgcag gcgagagagt ggatgcgatt ggccgagatg gacctcgagg tcgcgaacta
      661 tctgttcgga taccatcaaa tacccgttga aatcatctgt tatcacagtc agcagtccgc
      721 cgagaaatat ataaaggccc ttatggtttc atccggcgaa gagccgccta aaacccataa
      781 ccttatcgat cttgcccgga tatgcaaaga aaagaagatc gcagcggaga tatgggacgg
      841 catggcgagc gaaatcgtcg ggttgaatcg attcggcacc gtcccgaggt acccctccaa
      901 aatcgaactt gccgaatccg acgcccgcag ggcggtcgaa gatgcatcgt cgattcggga
      961 agttatcacg ggaagaatac cgtctcaaac gtcctgctcg ggaccgattt aaccgctgcc
     1021 tgcggtcgct catttcgagc ggcggcggca gcggatgcag gagaaacgcg atattgcggg
     1081 ttcacgctcc ccaagaacat cataaaggtg gaagaacgca aacgcccccg gaaaacctct
     1141 ccgggggcgc ttgcgtatct cgtggcggct gccgtcagaa acgaacaacc tccaggccga
     1201 ggcgctgctt catgaagcgc tcgagggcgt cgccgtggcg gccgaaggcg accatgtgat
     1261 ggcagccgag catggcgtcg aagcacgcct cccagctctt caggccgaag cggaactgcg
     1321 tccggcaggt gtcgagccgg gggttcgaga gcaggggcgc ctcgaagagc acggcgttcc
     1381 ggaactcgtt gccgatgcgg aagatggtcg cgtcgcgctc cccctccacc tccacccgct
     1441 gcgagacgga gagccccgtc tcgtggtgcg agcggatctc gtacggctgc gcgttcgcgg
     1501 agaggagctt ccggggcgcg gtgcagtgga cgatgtcgag cgtgttctcc aagttataca
     1561 cggggttccc catgaagacg ggcatcccgg tgatcgcctt gccgacgatc atggagacgg
     1621 cggagtccag gtccccctcg caggcggcca cggtctccgg ttcgtcgttg agcatcgaga
     1681 gcgcgacgca cgccgtgacg cccagcgtct tcgccagcga gaagcaggag atggcgagcg
     1741 cgttgcagcc gtagcgggcg agcaggttct tcagcgcccg gtacatccgg aacgcctgtt
     1801 cgatctgctc catgttcggc tccaggcagg aacgcgcgcc gtcggcgaac ttcttcgcga
     1861 gcgaggcgga gacggagtcg ttccggcacg tttcgaactc cgcgaacagc tcctccagcc
     1921 cgatatgcac cgacgagatg cccagcagcc gctccatctt ccgcgtgtcc acgctcgcgg
     1981 agaccaccca ctcctgcgtg taccccacca gcagcagacg cgcgctccgg accctgtgcg
     2041 cggctttcag ctcccgcgca gcggccagcc aggagccctc gtggtagacc ttcgccactc
     2101 tgcaccccct gttgcggagc gttccccaga cgtcggcgac ggcggggagg gcgttcttcg
     2161 agacgatgcg cgtaatcgtc ggccgctccc cctccggata gatgtcgtcg ttggggaaga
     2221 agctccagag caggctttcg gaggccgctc ccgcagcggc gagcatccgt ttctgcacgc
     2281 cccccgagag gggaacgagc agaggaaccg cccgcccgag cgaagagagc gcgtcgtcga
     2341 ggtcggcgtc tcccgccacg atgcgcgcgg tccgcatgag cgacgtcggc gcgtccatcg
     2401 cctccagcat tccggcgacg gccttccctg cggcgtccag ctccgccctg tcgggccagt
     2461 agacggggga ggcgaaaaga agaggctgca cgaagagttt gttgaccatg atccttcctc
     2521 cttctctctt actgcaatag cggcggatca ggcttcctgc gccccgccga ggtaggcgtc
     2581 cttcacggcg gggtcgcaga gcagcgcgcg cccctcgcct tcgagcgcca cgcgcccgtt
     2641 ctccagaacg taggcgtagt cggcgatggc gagcgcctgg ttggcgttct gctccaccag
     2701 cacgacggtg atcccctcgt cgcggatgcg ccgcaccgtg tcgaagaccc ctttcacgat
     2761 gatcggggcg agcccgagcg acggctcgtc gagaagcagc accttcggct ccgccatcag
     2821 ccctctgcag atggcgagca tctgctgctc ccctccggag agcgtccccg cctgctggcg
     2881 cgcgcgctcc ttcaggatgg ggtacatctc gaacatccgg tcgaggtttc tgcggacgcg
     2941 ccccgcgtcg ttcacggtgt acgcgcccgc catcaggttc tcgcggacgg tgagaccggg
     3001 aaagacctgc cgcccctcgg gaacctgcac cacgccgagc ttcaccacgc ggtacggctc
     3061 gcgcggcagc agcttcgcgg tgtccgggcc gattccttcc gtaaggaaga tttttccccg
     3121 ctcctgcttc accaggcccg agatcgtgtt cagcagcgtg ctcttccccg cgccgttgga
     3181 gccgatgatg ctgacgatgc tcccccgctt gacttcgagc gagactccaa gaagcgcc
//
LOCUS       DAOTIL010000233         3233 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminobacteriaceae bacterium isolate AMR_MDS_3026
            NODE_35208_length_3233_cov_3.476400, whole genome shotgun sequence.
ACCESSION   DAOTIL010000233 DAOTIL010000000
VERSION     DAOTIL010000233.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868050
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminobacteriaceae bacterium (wastewater metagenome)
  ORGANISM  Aminobacteriaceae bacterium
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae.
REFERENCE   1  (bases 1 to 3233)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3233)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:19:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,224
            CDSs (total)                      :: 3,198
            Genes (coding)                    :: 3,184
            CDSs (with protein)               :: 3,184
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3233
                     /organism="Aminobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_35208_length_3233_cov_3.476400"
                     /isolate="AMR_MDS_3026"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:3034609"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            18..713
                     /locus_tag="P5201_05940"
     CDS             18..713
                     /locus_tag="P5201_05940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006300244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HRV98108.1"
                     /translation="MDYLSEGFLQGFSLIFSGNEEVWSAVAVTLKLSGLSMLVTLLFG
                     IPAGFALGYFTFPGKSLLRLLVDTLLSIPTVVVGLFVYAFISNRGPLGQFELLFTIPG
                     MAIGQTILALPIVLALTASAVESADSRLRFTLLTLGARGRRLLFSTLWEGRHQILLAA
                     VTAYGRVTAEVGVSMMLGGNIKWHTRTVTTAITLETGKGEFSTGIALGVLLLCLSFVL
                     NWAVSFLRRRGAA"
     gene            742..1398
                     /locus_tag="P5201_05945"
     CDS             742..1398
                     /locus_tag="P5201_05945"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HRV98109.1"
                     /translation="MHKTPLYELTNIVCAYEDEPVLQVDSLAIPDEGPVAFVGHNGSG
                     KSTLLKLLSFLLPQVSGELRFLGETQDKKDDARLHATLLLQEPYLLRRSVFENIAYGL
                     RARGEASLRERVVEALEMVGLSADFARREWYQLSGGEARRVALASRLALRTRVLLLDE
                     PTANVDEENAQLMAEAVLDAWKRWNTTPLIATHDLQWLDTVTQRRIFMRRGKIERIAL
                     "
     gene            1610..2359
                     /gene="modA"
                     /locus_tag="P5201_05950"
     CDS             1610..2359
                     /gene="modA"
                     /locus_tag="P5201_05950"
                     /inference="COORDINATES: protein motif:HMM:NF024921.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009897 - external side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015412 - ABC-type molybdate transporter
                     activity [Evidence IEA];
                     GO_function: GO:0030973 - molybdate ion binding [Evidence
                     IEA];
                     GO_process: GO:0015689 - molybdate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdate ABC transporter substrate-binding
                     protein"
                     /protein_id="HRV98110.1"
                     /translation="MKLYRNRWVFSILAFFLVFGTALPSSAENAGTVAAAASLQSLMA
                     KTIPWFNEQRGTGLQGVYGASGNLARQIESGAPFDLFLSADEKWARYVEEKGLLDGEP
                     LPFAAMPLVLWHSGETAPSLEILRSEKLTVAIANPETAPFGRRAKAYLMEKGLFEAIE
                     TSGRLIIGGDVLKTGLAAKSGGADLAILPLSTASSLGEGSWTLLDAEPQTLFGGVVKG
                     RSTPAIEAFWTFLRSPEAAPFLKEFGFLPPR"
     gene            complement(2415..2708)
                     /locus_tag="P5201_05955"
     CDS             complement(2415..2708)
                     /locus_tag="P5201_05955"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRV98111.1"
                     /translation="MFTGVLSPYRSVCSGRLFFKNAVKDVIAMAPPHAPVKHDRPGMS
                     FFAQMLIVLLVLALLLLLVLVADTYLHDIGKDWSHSTLVKMILERREVVPLFE"
     gene            2986..>3233
                     /locus_tag="P5201_05960"
     CDS             2986..>3233
                     /locus_tag="P5201_05960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006300280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdate ABC transporter permease subunit"
                     /protein_id="HRV98112.1"
                     /translation="MLPPLLLSLRVLLFAVPLLVLLGGGIGWLLARANFPGKGFVSLL
                     VQLPLILPPSVMGFYLLFAIGRNPFLKSLGILFSFPALV"
BASE COUNT          582 a         1008 c          970 g          673 t
ORIGIN      
        1 cgaaggggtg tatatttttg gactatctct ccgaaggatt tctgcaaggg ttctcgctca
       61 tcttctcagg aaacgaggag gtttggtcgg cggttgcggt gacgctgaaa ctctccggcc
      121 tttcaatgct ggtgaccttg ctcttcggca tcccggcggg attcgccctc ggctacttta
      181 ccttccccgg caagagcctt ctccgtcttc ttgtagacac gctcctctcg ataccgaccg
      241 tggtggtcgg ccttttcgtc tatgcgttca tctcgaaccg ggggcctctg ggtcagtttg
      301 aactgctctt cacaattccc ggcatggcga tcggccagac catactggcg ctccccatcg
      361 tcctcgcgct caccgcctcc gcggtcgaga gcgccgacag ccgtcttcga ttcacgctgc
      421 tcacgctcgg tgcacggggc cgccgccttc tcttctccac gctctgggag gggcggcatc
      481 agatactgct cgcggccgtc acggcctacg gcagagtgac cgccgaggtg ggcgtctcga
      541 tgatgctcgg cggcaacatc aagtggcata cgcggaccgt caccaccgcc atcacgctgg
      601 agacgggcaa gggagagttc tccactggca tcgctctcgg cgtgctgctg ctctgcctct
      661 cgttcgtcct caactgggcc gtttccttcc tgcgccggag gggcgcggcg tgatccccgc
      721 cgaaaggatg tgcgcgctgt gatgcacaag acgccgctct acgaacttac aaacatcgtc
      781 tgcgcctatg aggacgagcc cgttctgcag gtggattcgc tcgccatccc cgacgaaggg
      841 cccgttgcgt tcgtcggaca caacggaagc ggcaaaagca cgctgctgaa gctgctctcc
      901 ttccttctgc cgcaggtgtc gggcgagctg cgcttcctgg gggagactca ggacaaaaaa
      961 gacgacgccc gtctgcacgc gacgcttctc cttcaggaac cctacctcct ccgccgttcc
     1021 gtcttcgaga acatcgccta cgggctgcgg gcgcgggggg aggcatcact gcgggagcgc
     1081 gtggtcgagg cgcttgagat ggtggggctc tccgccgact tcgcacggag agagtggtac
     1141 cagctctctg gaggagaggc ccgccgcgtc gcgctcgcct cgcgcctggc gctgcggacg
     1201 agagtgcttc ttcttgacga gccgaccgcg aacgtcgacg aggagaacgc gcagctcatg
     1261 gcggaggccg tcctcgacgc ctggaagcgc tggaacacca cgcctctgat cgcgacgcac
     1321 gacctccagt ggctggacac ggtgacacag cgaaggatct tcatgcgccg gggaaagatc
     1381 gagcgcatcg cactgtaaaa caaaagggcc ttcgcgctcc agacaaccgg gagaaattcc
     1441 ctgccctcga aaagtgcgga gaggggctgg agaaagagag aagttagggg gaggattctt
     1501 tgaaatcctt cccttttttc ggtcgccata ctttttcgca accgagttgc attttttgcg
     1561 aacgaagcct agaataagcg caactttgtt gcaaaaggga ggaacaggta tgaaattgta
     1621 tcgaaacagg tgggttttca gcattctggc tttctttctc gtattcggca cagcgctccc
     1681 ttcgtcggcg gagaacgcgg ggacggttgc ggcggcggcc tcgctgcaat cgttgatggc
     1741 aaaaaccatc ccctggttca acgaacagcg cggaaccgga ctccaggggg tgtacggcgc
     1801 ttcgggcaat ctggcgcggc agatcgagtc gggcgcgccc ttcgatcttt ttttgagcgc
     1861 cgatgaaaaa tgggcccgtt acgtcgagga gaaggggctg ctcgatggag agccgctccc
     1921 cttcgcggcg atgccccttg tgctctggca ctccggagag acggcgccct ctcttgagat
     1981 tctgcgctcg gaaaagctca ccgtggctat cgcgaatccg gaaacggcgc ccttcggcag
     2041 gcgggcaaag gcgtacctca tggaaaaagg actttttgaa gcgatcgaaa cgagcggcag
     2101 actgatcatc gggggcgacg tgctcaagac ggggctggcg gcgaaaagcg gcggcgcgga
     2161 tctcgccatc ctgccgctga gcaccgcctc ctcgctcggc gaaggatcgt ggacgctgct
     2221 cgacgcggag ccgcagacgc tcttcggcgg ggtcgtcaag ggacgaagca ctccggcgat
     2281 cgaggcgttc tggacgttcc tccgctcgcc tgaagcggcg ccgttcctga aggagttcgg
     2341 tttcctcccg cctcgttgaa cactcggaat cgaaaagtga actcacgact tcagttcacg
     2401 cgggagcgga ggggctactc gaaaagaggg acgacctccc tccgctccaa aatcatcttc
     2461 acgagcgtcg agtgcgacca gtctttcccg atgtcgtgca gatacgtgtc ggcgacgagg
     2521 acgagcaaga ggagcagcgc gaggacaagc agaacgatca gcatctgagc gaaaaaggac
     2581 attcccgggc ggtcgtgctt caccggagcg tgtggcggcg ccattgcgat cacatccttg
     2641 acggcattct tgaaaaatag tcttccggag catacactcc ggtacggcga aagaacaccc
     2701 gtgaacatgc cggaaggtct tccgctacca ctattctaca acatgccgcg cgcaatcggc
     2761 ggaacggcgc gctatgccgt accgcctccg tccgttcttc gatctgaaac gattttgatg
     2821 caacgccgtt ttcctgatac catgagcaca ttctgcattt ctggaggtct gccgcatgtt
     2881 cgatgaactt ttcctctcct ctctcttccg gatatcgggg ctcgccccgc tctatcccct
     2941 ctgttccggg cgctccacgg ctcgcgcgag cagagcgtga cggcgatgct ccctccgctc
     3001 ctcctcagcc tccgcgtcct gctgttcgcc gttcccctgc ttgttctcct tggaggaggg
     3061 atcggctggc tgcttgcgcg ggctaatttc cccgggaagg ggttcgtctc gctcctcgta
     3121 cagcttccgc tgatcctccc tccctcggtg atgggcttct acctgctctt tgccatcgga
     3181 agaaacccct tcctgaagtc gctcggcatc ctcttctcct tcccggcgct cgt
//
LOCUS       DAOTIL010000234         3221 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminobacteriaceae bacterium isolate AMR_MDS_3026
            NODE_35394_length_3221_cov_4.085913, whole genome shotgun sequence.
ACCESSION   DAOTIL010000234 DAOTIL010000000
VERSION     DAOTIL010000234.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868050
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminobacteriaceae bacterium (wastewater metagenome)
  ORGANISM  Aminobacteriaceae bacterium
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae.
REFERENCE   1  (bases 1 to 3221)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3221)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:19:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,224
            CDSs (total)                      :: 3,198
            Genes (coding)                    :: 3,184
            CDSs (with protein)               :: 3,184
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3221
                     /organism="Aminobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_35394_length_3221_cov_4.085913"
                     /isolate="AMR_MDS_3026"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:3034609"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            1..168
                     /locus_tag="P5201_05965"
     CDS             1..168
                     /locus_tag="P5201_05965"
                     /inference="COORDINATES: protein motif:HMM:NF014860.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRV98113.1"
                     /translation="MLGVHIMGPEAASLLGEATLAVAKHLTAEDVANTIHAHPTLCEC
                     FRDACRRVMEE"
     gene            complement(316..1431)
                     /locus_tag="P5201_05970"
     CDS             complement(316..1431)
                     /locus_tag="P5201_05970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006302045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HRV98114.1"
                     /translation="MASEQAESKGKIMIVDDTVVNLELLQNLLQRRGYSIMAFPQGKL
                     ALKAALKSPPDMLLLDINMPEMDGFEVCSHFKSEPSLAKIPVLFISALEASQDKVRAF
                     AAGGVDYVTKPFQIEELNARVDTHLKIVRMQRELERYNHELEELVQEKVRDIEESHLS
                     TILAMVKLTEYRDVGTGMHIERTRTLCSMLAAKLAEHPEYDGLLSPEYAETIYNAAPL
                     HDVGKVGVPDGILLKPGRLTDDEWEIMKTHSEIGAETLREVRRKYVSNAFVNMGIEIA
                     LFHHEKWDGSGYPEGRSGEAIPLSARIMAVADVYDALRTRRPYKEPFSHEESFAILQK
                     DSGSHFDPLLVEVFAQLEDEIKDLYNRCADLDGSSCR"
     gene            complement(1421..>3221)
                     /locus_tag="P5201_05975"
     CDS             complement(1421..>3221)
                     /locus_tag="P5201_05975"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HRV98115.1"
                     /translation="PDIIFYKNTDGVFLGCNRPHEEMTGRSESETVGKTDYDFYDRET
                     ADFYREKDKEALAAERPLHNEEWVTYPDGRRALLDTLKTSFRGKDGELIGLLGISRDI
                     TERKRMEEDLRAALEAANAASLAKSAFLASMSHEIRTPMNAILGFSQLLERDPELAPK
                     HMKYVRIINRSGEHLLALINDILDMAKIESGRMTLNRAPFSPFAMLRDIVSLFHTLAE
                     EKSLSLTIEHDDDLRAATLLGDEGKLRQVLVNLVGNGLKFTQQGSVTVRFRTETDFRD
                     ASSDERELLLSVEVEDSGVGISPEQIELIFAPFQQSDSGKKAGGTGLGLTISRKFVEM
                     MGGTLTCESELGKGSCFRFRIPVERVYDVSLRPSQLGGRVLGLKPESGRPLILIADDD
                     DTSRGLLTELLTEAGFDCAEAANGLQAVELCASRTPDAVLMDMNMPLLDGYEATRMIK
                     ESDSRNIPVIALTADAFARDAVAACGADEYLRKPFVADELFAVLQTVLGLEYIYEDVS
                     GRDTAGGEAGSEAGGPGSPKAIDLSLIPGNLLRELHGAVEDGDMYRIRELIRSISAVD
                     EKVGKELLLLAERYDYERLLELVNDEGGLSDGE"
BASE COUNT          572 a         1060 c          918 g          671 t
ORIGIN      
        1 atcctcggag ttcacatcat ggggccggag gccgcctccc tcctgggaga ggctacgctg
       61 gcggtcgcga agcatctcac cgccgaggac gtcgcgaaca cgatccacgc ccaccccacc
      121 ctgtgcgaat gtttcaggga cgcctgccgc cgcgtaatgg aggagtaggc gtcccccgca
      181 gaaacgtcat cgccggagcg gaatctcctg ttccttcaca tacacacaac gcggcccttc
      241 gcaagagtgc gaggggccgc gtgctgtctc accgagcgcc gggacttcaa tagcgagcgc
      301 cgcttcccgg cggcgtcagc ggcaggaaga tccgtcgaga tcggcgcagc gattgtagag
      361 gtccttgatt tcgtcctcca gctgcgcgaa cacctcgacc agcagcgggt cgaaatgact
      421 ccccgaatcc ttttgcagga tggcgaaact ttcctcgtga gagaagggct ccttgtacgg
      481 acgccgcgtc cggagcgcgt cgtagacgtc ggcgacggcc atgatgcgcg cgctcagtgg
      541 aatagcctcc cccgagcgcc cttcggggta gccgctgccg tcccacttct catgatggaa
      601 aagagcgatt tctatcccca tattcacgaa cgcgttcgag acgtacttcc tccgcacctc
      661 ccgaagcgtc tccgcgccga tctcggagtg cgtcttcatg atctcccact cgtcgtccgt
      721 cagccgcccc ggtttcagca ggatgccgtc cggaacgccg accttgccga cgtcgtgcag
      781 cggagcagcg ttgtagatgg tctccgcgta ctccggggag agaagaccgt cgtactccgg
      841 atgctccgcg agcttcgcgg caagcatact gcacagggtc cgcgtgcgct cgatgtgcat
      901 tccggtgccg acgtcccggt actccgtcag cttcaccatc gccagtatcg tggagaggtg
      961 cgactcttcg atgtccctga ccttctcctg caccagttcc tccagctcat gattgtagcg
     1021 ttcgagctcg cgctgcatcc ggacgatctt caggtgcgta tcgacgcgcg cgttcagctc
     1081 ctcgatctgg aagggtttgg tcacataatc caccccgccc gccgcaaacg cgcgcacctt
     1141 gtcctgggag gcttcgagcg cgctgataaa gagcacggga attttcgcca gcgagggctc
     1201 cgacttgaag tggctgcaca cctcgaaacc gtccatctcg ggcatgttga tgtccagcag
     1261 gagcatatcc ggaggactct tcagggccgc cttcaacgca agcttcccct gaggaaacgc
     1321 catgatgctg taccctctgc gttgcagcag gttttgcagc agttcgaggt tcacgacggt
     1381 atcgtcgacg atcattatct tccctttgct ttcagcctgt tcactcgcca tcggagagtc
     1441 ctccttcatc gttcaccagc tccagcagtc tctcgtagtc gtaccgctcg gcgaggagaa
     1501 gcagctcctt ccccaccttt tcgtccactg cggatatgct ccggatcaac tccctgatgc
     1561 ggtacatatc gccgtcttcc accgcgccgt gcaattctcg cagaagattc cccggaatca
     1621 gggaaagatc gattgctttc ggggagcccg ggccgccggc ttccgacccg gcctcgcccc
     1681 cagccgtatc tctgccggat acatcttcgt agatatattc cagtccgaga accgtctgca
     1741 gtacggcgaa gagctcgtcc gccacgaagg gcttgcgcag atactcgtcg gcgccacacg
     1801 ctgcgacggc gtcgcgggca aaggcgtcgg cggtaagcgc gattacaggg atattcctgc
     1861 tgtcgctctc tttgatcatc ctcgtggctt cgtacccgtc gaggagcggc atattcatat
     1921 ccatcagcac agcgtccggc gtgcgggatg cgcacaattc gacggcctgg agaccgttcg
     1981 ccgcctcggc gcaatcgaat cccgcttcgg tgagaagttc ggtcaaaagt ccgcggcttg
     2041 tatcgtcgtc gtccgcgatc agaataaggg gacgcccgct ttccggtttc agcccaagga
     2101 cgcgcccgcc gagctgcgac ggccgcaggg aaacgtcgta caccctctcc accggtatcc
     2161 ggaagcggaa acacgacccc ttgccaagtt cgctctcgca ggtcagggtt ccgcccatca
     2221 tctccacgaa cttccggctg atggtgaggc cgagtccggt gccgcccgcc ttctttccgg
     2281 agtcgctctg ctggaacggc gcgaaaatga gttcgatctg ctccggagag atgccgacgc
     2341 cgctgtcctc aacctccacc gagagcagga gttcgcgctc atccgaggaa gcatcccgga
     2401 aatcggtttc cgtgcggaaa cgaacggtca cgcttccctg ttgcgtgaac ttgaggccgt
     2461 ttcccacaag attgacgagc acctgccgca gttttccctc gtcgcccaga agcgtcgccg
     2521 cgcgcagatc gtcgtcgtgt tcgatcgtca gggagagcga cttctcttcc gccagcgtat
     2581 ggaagagcga gacgatatcc cgcagcatgg cgaacgggct gaacggcgcc ctgttcaagg
     2641 tcatcctgcc ggattctatc ttcgccatgt cgagaatgtc gttgatcagc gcgagcaggt
     2701 gctccccgct gcgattgatg atccgcacgt acttcatgtg tttcggagca agctccgggt
     2761 cgcgctccag gagctgcgag aagcccagaa tcgcgttcat cggcgtccgg atttcatggc
     2821 tcatgctcgc caggaaggcg ctcttcgcca ggctcgcggc gttcgccgcc tcaagggctg
     2881 cgcgcagatc ctcctccatc cgtttgcgtt cggtgatgtc gcggctgatc ccgagaagac
     2941 cgatcagctc gccgtccttc ccccggaagg acgttttgag cgtgtcgaga agcgcgcgcc
     3001 tcccgtccgg gtaggtcacc cactcctcgt tatgcagcgg ccgctcggcg gctagcgctt
     3061 ctttatcctt ctcccggtag aaatcggcgg tttccctgtc gtaaaaatcg taatccgtct
     3121 tcccgaccgt ctccgactcg cttctacccg tcatctcctc gtgaggccgg ttgcacccga
     3181 ggaagacccc gtcggtattt ttgtaaaaaa tgatatcggg g
//
LOCUS       DAOTIL010000235         3221 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminobacteriaceae bacterium isolate AMR_MDS_3026
            NODE_35397_length_3221_cov_3.337966, whole genome shotgun sequence.
ACCESSION   DAOTIL010000235 DAOTIL010000000
VERSION     DAOTIL010000235.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868050
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminobacteriaceae bacterium (wastewater metagenome)
  ORGANISM  Aminobacteriaceae bacterium
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae.
REFERENCE   1  (bases 1 to 3221)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3221)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:19:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,224
            CDSs (total)                      :: 3,198
            Genes (coding)                    :: 3,184
            CDSs (with protein)               :: 3,184
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3221
                     /organism="Aminobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_35397_length_3221_cov_3.337966"
                     /isolate="AMR_MDS_3026"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:3034609"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(18..665)
                     /locus_tag="P5201_05980"
     CDS             complement(18..665)
                     /locus_tag="P5201_05980"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRV98116.1"
                     /translation="MLKVRSIFNCLEYAGALPALPIRRSLFLFFAVFCFAAFAASASA
                     RTVSPQADGRNAETVPFSSIREAYRQKAFDLWKRAADLQIGGRYEEALEKYREGLGYH
                     EDPRVREHVWKLEETLAARRGSENARKQAARRAAAELWKEAAALQQAGWFEDALQKYK
                     EGLELSDDQRVREHVKKLEEYIERLRHRGLLPVLVFMRERSGDTLLKTPSSGVFL"
     gene            complement(785..1330)
                     /locus_tag="P5201_05985"
     CDS             complement(785..1330)
                     /locus_tag="P5201_05985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013049063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="folate family ECF transporter S component"
                     /protein_id="HRV98117.1"
                     /translation="MKQSRYALRDMVVLSLLIGLNVVLTRIASIRIPIGGVESLRIGF
                     GAVPVIFAGLFFGPRAGAVVGALGDVAGFIMSPAGGAFMPHFTLTAALTGAIPPLALR
                     LFRHREHVAEHSFIELLVAIAAGQLITSVLMVPYFLNMLFGVPFTATLIVRLQTQLLQ
                     IPLFVMCIKLLARRLSFIVAG"
     regulatory      complement(1398..1497)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01831"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="THF riboswitches regulate genes encoding folate
                     transporters and folate metabolism; They are most commonly
                     found in Clostridiales and Lactobacillales; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /bound_moiety="tetrahydrofolate"
                     /db_xref="RFAM:RF01831"
     gene            complement(1661..2809)
                     /locus_tag="P5201_05990"
     CDS             complement(1661..2809)
                     /locus_tag="P5201_05990"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRV98118.1"
                     /translation="MKMFKILMIVVVILGCASVSVGAENEDDSIFAKMWSKAMPKLEQ
                     ALELFERGETLPESKIFGADKQSNNAKYEKLLNEVFDILLDSEFHASRGEYRSLEERI
                     EKSEQKIAELRKERIGAPEESWIPFRQTRSKIDEEVDELKEDIARFRKEKEAIRSSMV
                     AALSAMGVSVDGAKLDILLASVSGDDVIALLLAADSIKKINLQIVDLLRKGEGNIHMA
                     RKYTGVHMVLVDIYLHAHHDVLEKIASEYSPRLDSITSSAQLLKKQAEQMLAATPRGG
                     SQREVLETNVKANERTVQTASLYRDYLRTQQNKLRISAEKLTSDLSVARNTYQTVKTG
                     GDLISLINISMRTLESVFGFVLPDLSTVYDQEVLKEFERITGQIRKGS"
     gene            2971..>3221
                     /locus_tag="P5201_05995"
     CDS             2971..>3221
                     /locus_tag="P5201_05995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014163710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003941 - L-serine ammonia-lyase activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine dehydratase beta chain"
                     /protein_id="HRV98119.1"
                     /translation="MKLLKRVSILNHVIGPVMRGPSSSHTAGPWRIGRMARELLGARP
                     VLARFYFHPSSSLAVCYHDQGSDRAFIAGLLEIPLTSSD"
BASE COUNT          767 a          936 c          773 g          745 t
ORIGIN      
        1 tttttgacgt accactttta caaaaaaacg cccgatgagg gcgtttttaa aagagtatct
       61 ccacttcgtt ctctcatgaa aaccagaacc ggaagcagtc ctcgatgccg aaggcgttcg
      121 atgtactctt caagtttttt cacatgctcg cgcacccgct ggtcgtcgga taattccagc
      181 ccctccttgt acttttgaag cgcatcctca aaccaccccg cctgctgaag cgcagcggcc
      241 tccttccaga gctccgccgc cgccctgcgc gcggcttgtt ttctcgcgtt ctcactgcct
      301 cttctcgccg caagcgtctc ctcaagcttc cagacgtgct ctctcacccg tggatcttca
      361 tggtatccaa gcccttcccg atacttctca agcgcttctt cgtaccgtcc gccgatctgc
      421 aagtcggcgg cccgcttcca gagatcaaac gccttctgac ggtaggcctc gcgaatcgag
      481 gagaagggaa cggtttccgc gttacgcccg tcagcctgcg gagaaacagt cctggctgac
      541 gcagacgccg cgaaggccgc gaaacaaaac actgcaaaaa agaggaacaa acttcttcgg
      601 attggcagcg ccggcagagc gccggcatac tccaaacaat taaagatgct gcgcactttc
      661 aacatgcttt ctcccctcct cctccggtcg acgacgtcgc attgaggaac cgacgaaaaa
      721 acgcgatcaa aaaaagacgc tcttctcggg acgtttcaga agggcgtctt tactttttca
      781 ttggttaccc ggcgacgatg aacgagagac ggcgagcgag cagtttgatg cacatgacga
      841 agagcgggat ctggagcagc tgcgtctgga ggcgcacaat cagcgtcgct gtgaacggaa
      901 cgccgaagag catattcaga aaatacggaa ccataagcac cgaggtgata agctgtcccg
      961 ccgcgatggc aaccagcagc tcaatgaagg agtgttcggc gacgtgttcc cgatgccgga
     1021 agagcctgag cgccagcgga gggatagccc ccgtgagcgc cgccgttagt gtgaagtggg
     1081 gcatgaaagc accgcctgcg gggctcatga tgaatccggc gacatcgccg agcgcgccga
     1141 cgactgcccc tgcccgcgga ccgaagaaga ggcccgcgaa gatcaccgga acagcgccaa
     1201 aaccaatcct gagcgattcg acgcccccga tggggatacg aatgctcgca atacgagtca
     1261 ataccacatt caaaccgatc aaaagggaaa ggacaaccat atcgcggaga gcataccgag
     1321 actgtttcat gaaaaaaacc tccttatgac ataccggcac aaccgccaca tcaaaagagg
     1381 tctgaaccaa ttgatgtgac agcgaacgcg acttccccac cgcaaatcac gaatgatttt
     1441 tcgtcccggg gcaacgtccc atcccagggc acttcacgcg ggaaaaccta ctctgcctac
     1501 ctttattcgt agcattatac catctttcaa gaaaaaacaa gcgcacatcg tgctgcatat
     1561 tgtcaagaat ccgagggctg cagaacactt gagaggcgta caagtaaaaa gcaagcgccg
     1621 cttccagatg ctgtttcatc cgggaaacgg cgcttcaggc tcaactccct ttgcgtatct
     1681 gccctgtaat acgttcgaac tccttgagca cttcctggtc gtacaccgtg gaaagatccg
     1741 gaagcacaaa accgaagacg ctttcgagcg tccgcatgga gatgttaatc agggatatca
     1801 gatcgccgcc ggtttttact gtctggtatg tattccgcgc aaccgaaaga tcgctcgtga
     1861 gtttctctgc cgagatacga agcttattct gctgcgttcg aaggtaatcc ctgtacagag
     1921 acgccgtctg gaccgttcgt tcattcgcct ttacgttcgt ctccaacact tcgcgctgac
     1981 tgccgcccct tggtgttgct gccagcattt gctccgcctg tttttttaag agctgcgcag
     2041 aacttgtgat cgaatcaagc cgcggcgagt actccgaggc gatcttctca agaacgtcgt
     2101 gatgcgcatg aagatagata tccacaagaa ccatgtgaac gccggtatac tttctggcca
     2161 tgtggatatt cccttctccc ttgcgcagga gatctactat ctggagatta atcttcttta
     2221 tgctgtccgc ggcaagcaga agagcaatga catcgtcgcc gctcacactt gcaagcagga
     2281 tatccaattt ggccccgtcg acgctaactc ccatggcgct taacgcagcc accattgaag
     2341 aacgaatcgc ctctttttct ttcctgaagc gagcaatatc ctccttgagc tcatcaactt
     2401 cctcgtcaat tttggaacgt gtctgcctga agggaatcca gctctcctcc ggggcgccga
     2461 tgcgttcctt tcgcagctcc gcaatcttct gctccgattt ctctattctc tcttccaaag
     2521 aacggtactc gcctctggag gcatggaact ccgaatcgag aaggatgtcg aagacctcat
     2581 tcaagagctt ctcatacttt gcgttattgg actgtttatc ggcgccgaag atctttgatt
     2641 caggcagagt ttccccgcgc tcgaagagct cgagagcctg ttcgagtttc ggcatagcct
     2701 tactccacat tttggcaaag atggaatcgt cttcgttttc cgccccgacg cttaccgaag
     2761 cgcaccccag tataactact acaatcatga gtatcttgaa catcttcata gttatcacct
     2821 cgtgagagta atctatcaca aaaaagcttt tctttgttcg tcattcctgc gtaccgcaaa
     2881 aaaagtttcg tgcgacctcg acgatcaggc gttttccata agctcgcgct tcaaatgata
     2941 tactctttta agcaaaacgt cattccggag gtgaagcttt tgaaacgggt aagcatactg
     3001 aatcatgtca tcggcccggt tatgcgcggg ccttcaagtt ctcacacggc aggtccatgg
     3061 cgaatcggaa gaatggcgcg agaactgctc ggcgcccgcc ctgtgctggc gcgcttttac
     3121 ttccatcctt caagctctct ggcggtctgt taccacgatc agggaagcga tcgcgcattc
     3181 attgcaggac tccttgaaat accgcttacc tcttccgatt t
//
LOCUS       DAOTIL010000236         3220 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminobacteriaceae bacterium isolate AMR_MDS_3026
            NODE_35419_length_3220_cov_3.226224, whole genome shotgun sequence.
ACCESSION   DAOTIL010000236 DAOTIL010000000
VERSION     DAOTIL010000236.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868050
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminobacteriaceae bacterium (wastewater metagenome)
  ORGANISM  Aminobacteriaceae bacterium
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae.
REFERENCE   1  (bases 1 to 3220)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3220)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:19:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,224
            CDSs (total)                      :: 3,198
            Genes (coding)                    :: 3,184
            CDSs (with protein)               :: 3,184
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3220
                     /organism="Aminobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_35419_length_3220_cov_3.226224"
                     /isolate="AMR_MDS_3026"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:3034609"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            53..1594
                     /locus_tag="P5201_06000"
     CDS             53..1594
                     /locus_tag="P5201_06000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013048382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0043163 - cell envelope organization
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="WecB/TagA/CpsF family glycosyltransferase"
                     /protein_id="HRV98120.1"
                     /translation="MAILAAVFGMVQHMYPNSLWKPVYLLIGFIFALFGPRIAFIGLP
                     AGQYHYLPVEVSVLITALWIAAFPVLLQELDNIPGMAGHLLAVSFSLLLTATVFSGQS
                     LQDAFFMSLSGLLFLGVFWSRHGHMYRRMGEALAAMWGVLVAGTSVLGVSKGITFGTL
                     MVLPLGLFAIPIMEASLNFMNRAFSSKSPNAAVLYSKLIGKGLDHPSAVRFITSLCAL
                     GGSAVAISQLGHPLSMMFVVSLAILFAGITLAPAIRDMKENVEMHQRPSIWRTTIDNV
                     SMNYALAKARGALASRSGARLVATVNALAVMEAEKNDKYANALDGSFLTLADGAGLVW
                     ALKLLGTPVQERVTGIDYMTGLARMAAAEGWPVYLLGSKPLIVEKAAFELQRRFPDLT
                     VAGFRDGYFDEGDVSVIDEIRESGAKILFVAMGIPKQEIWVTENAERLGDLLAVGVGG
                     AFDVVSGTLKRAPELLQKIGLEWFYRLLQEPWRWKRDLDLFVFVIKVLLTKTGLYRSR
                     ARRTV"
     gene            1740..2201
                     /locus_tag="P5201_06005"
     CDS             1740..2201
                     /locus_tag="P5201_06005"
                     /inference="COORDINATES: protein motif:HMM:NF014629.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (pseudouridine(1915)-N(3))-methyltransferase RlmH"
                     /protein_id="HRV98121.1"
                     /translation="MKIIILSVGKPKDVHVVSLAEHYFKRLRSFVPVVLESVPEAKDP
                     LPSKKVEREGVGLLKNLRERDFLVTLEERGKCFDSREFAEWVQTSVERTPGRVVLAIG
                     GAFGLSEDVRRRSDFSLSLSPMMMPHELCLVFLMEQLYRAFTLIRGVEYHH"
     gene            2223..2528
                     /locus_tag="P5201_06010"
     CDS             2223..2528
                     /locus_tag="P5201_06010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009201136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YbaB/EbfC family nucleoid-associated protein"
                     /protein_id="HRV98122.1"
                     /translation="MNMEKIMKQAQKMQAQMAKIQEDLAGEQVEGSAGGGMVTVTCNG
                     QGDVVAVKIEPEVAGDDVEMLEDLVLAAVNDAIRKSKELANSRMSQLTGGLGMPGLF"
     gene            2548..3156
                     /gene="recR"
                     /locus_tag="P5201_06015"
     CDS             2548..3156
                     /gene="recR"
                     /locus_tag="P5201_06015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013048369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombination mediator RecR"
                     /protein_id="HRV98123.1"
                     /translation="MALPDSFERLASLFRRMPGVGAKSARRMAFFVLQQPPSFSDELA
                     RVLVALKERVHACSVCGNITDDDPCSICSDPLRDHRLLCVVETVEDLFSIEHAGVFAG
                     VYHVLGGRVSPLDGETLDERVLSALKEHIENDAIEEVIIATNPRIEGDLTFHALVEYL
                     GANNVALSRIAYGLPVGGSIEFADRTTLQAAMDSRVRVRRSS"
BASE COUNT          645 a          798 c          931 g          846 t
ORIGIN      
        1 gatccttgcc gcactatgcg tcggggggcc gtcttcccgt ttcatcgtcg gaatggcgat
       61 ccttgcggcg gtattcggca tggtgcagca tatgtatccg aactcgctct ggaaaccggt
      121 ctacctgctt atcggtttta tttttgctct tttcggtccg cgcatcgcct ttattggttt
      181 gcccgccggg cagtatcact atttgccggt tgaggtctcc gtactgataa ccgccctctg
      241 gattgccgcc ttccccgtgc tgcttcagga actggataac attccgggca tggccgggca
      301 tctgcttgct gtttcgttct cccttctgct gaccgccacg gtcttttccg gtcagagttt
      361 gcaggatgct ttttttatgt ccctgtcggg cttgctcttc ctcggagttt tctggagccg
      421 tcacgggcac atgtaccgaa gaatgggaga ggcgctcgcc gcgatgtggg gcgttttggt
      481 cgccggcact tccgttctcg gcgtgagcaa aggtattacc ttcggtactc tgatggtgct
      541 ccctctaggt ctttttgcga ttcccatcat ggaggcgtcg ctgaatttca tgaaccgtgc
      601 cttttcctcg aagtcgccga atgcggccgt actgtacagc aaactcatag gaaagggact
      661 tgatcatccc agcgccgtcc gcttcatcac gtcgctctgc gctctcggag ggtcggccgt
      721 cgccatctcc cagcttggac atcctctctc catgatgttt gttgtgagcc tcgctattct
      781 tttcgccggt attactttgg ctccggccat acgcgatatg aaggagaatg tggaaatgca
      841 ccagagacct tcaatttggc ggactactat tgacaacgtt tctatgaact atgcgcttgc
      901 aaaagcccgc ggagcccttg cctcaaggag cggcgcacgc cttgtcgcga cggtaaacgc
      961 acttgccgtg atggaggccg agaagaacga caagtatgcg aacgctttag acggttcgtt
     1021 cttgaccctt gccgacggcg ccggtctcgt atgggcgctc aagctgctcg gtacgccagt
     1081 gcaggagaga gtgaccggaa tagactacat gaccggactc gctcgtatgg ctgctgccga
     1141 agggtggccg gtctatcttc tcggttcgaa gcctttgatc gtggagaaag cggcgttcga
     1201 gttgcagcgc cggtttcccg atctcactgt tgcgggtttc cgcgacggat attttgacga
     1261 gggcgacgtt tccgtgattg atgagatacg agagagcggc gcaaaaatcc tttttgttgc
     1321 tatgggtatt cccaagcagg aaatatgggt aaccgaaaac gccgaacggc tgggcgatct
     1381 tcttgccgta ggcgtcggcg gtgctttcga cgtcgtgtcg ggcactctga aacgagctcc
     1441 ggagcttctt cagaaaatcg ggcttgaatg gttctaccgt cttcttcagg agccttggag
     1501 atggaaaagg gatcttgacc tttttgtgtt tgtgataaaa gtccttctga cgaaaacagg
     1561 tctttaccgt tcacgagcaa gacgaacggt gtaatgaaac tccttctgaa aaattggctc
     1621 cctctgtatt atgtgcgccc tgatgttttc tgcgaagacg gaggggcgcg ttttttgttg
     1681 tccggttcac aggatcgatc gacaaagaga aaatacgaga aacgtgctgg acatatacta
     1741 tgaaaatcat tatccttagt gtcggaaaac cgaaagatgt tcatgttgtt tcgctcgcgg
     1801 aacactactt taaacgtctt cgttcttttg ttcctgttgt gcttgagagc gtgcccgaag
     1861 caaaggaccc gcttccttcg aagaaagtgg agcgcgaggg agtcggcctt ttaaagaatc
     1921 ttcgcgaaag agatttcctg gtaacgcttg aagagcgggg aaaatgcttt gacagccgtg
     1981 agtttgccga atgggtgcaa actagtgttg agcgaacgcc gggaagagtt gttctggcga
     2041 taggaggcgc gttcggcctt tcggaggatg ttcggcgtcg gagcgacttt tcactttctc
     2101 tttcgccgat gatgatgccg cacgagctgt gcctcgtttt tctcatggag cagctttatc
     2161 gtgcgtttac tctgattcgc ggcgtggagt atcatcatta aaaaaggagt tgtgcttttg
     2221 atatgaatat ggaaaaaatt atgaaacagg cgcagaagat gcaggcccag atggcgaaaa
     2281 ttcaggagga cctcgcaggc gagcaggttg aggggagcgc ggggggcggt atggtcacag
     2341 tgacctgcaa cggacagggg gatgtcgttg cagtcaagat cgagcccgag gttgcagggg
     2401 acgatgttga gatgcttgag gatcttgtac ttgccgctgt gaacgacgcg atccgtaaga
     2461 gcaaggaact tgcgaacagc aggatgagtc agctgacggg cggccttggt atgcccggtc
     2521 ttttctaggg cgttcccggg cttctccgtg gctcttcctg attcctttga aagacttgca
     2581 tcattgtttc ggagaatgcc gggtgtcgga gcaaaaagcg cccggcgtat ggcttttttc
     2641 gtcctgcagc agccgccttc tttttccgac gagctcgccc gcgtccttgt cgcactgaag
     2701 gagcgcgttc atgcatgttc cgtttgcggc aacataacgg acgatgatcc ctgttcgatc
     2761 tgctccgacc ctcttcggga ccaccgtttg ctctgtgtgg tggaaaccgt cgaggacctt
     2821 ttcagcatcg aacacgcggg tgtttttgcc ggggtatacc atgttcttgg aggacgagtc
     2881 tctcccctgg acggcgagac gctcgacgag cgcgtcctgt ccgccttgaa agagcacatt
     2941 gaaaatgatg ctatcgagga ggtgatcatt gccactaatc cgcgtattga aggcgatctg
     3001 acattccacg ctctagttga atacctcggc gccaacaacg tcgctctttc aaggattgcg
     3061 tacgggcttc ctgtcggagg cagtatcgag ttcgctgatc ggacgacgct gcaggcggcg
     3121 atggattccc gcgtacgagt caggcgatct tcctaattgt gtcttggaaa aggagacgtg
     3181 ctgtgaaaaa gtttaggctg atgacatatt tgtcaaaaaa
//
LOCUS       DAOTIL010000237         3220 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminobacteriaceae bacterium isolate AMR_MDS_3026
            NODE_35420_length_3220_cov_3.088784, whole genome shotgun sequence.
ACCESSION   DAOTIL010000237 DAOTIL010000000
VERSION     DAOTIL010000237.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868050
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminobacteriaceae bacterium (wastewater metagenome)
  ORGANISM  Aminobacteriaceae bacterium
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae.
REFERENCE   1  (bases 1 to 3220)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3220)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:19:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,224
            CDSs (total)                      :: 3,198
            Genes (coding)                    :: 3,184
            CDSs (with protein)               :: 3,184
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3220
                     /organism="Aminobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_35420_length_3220_cov_3.088784"
                     /isolate="AMR_MDS_3026"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:3034609"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..200)
                     /locus_tag="P5201_06020"
     CDS             complement(<1..200)
                     /locus_tag="P5201_06020"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRV98124.1"
                     /translation="MNGRRNISAFSVLMLLTLLALPGRAFAASMIVAVQPFLAQGTSF
                     HLGATVGDIVSTSLAGNRDITLV"
     gene            complement(239..958)
                     /locus_tag="P5201_06025"
     CDS             complement(239..958)
                     /locus_tag="P5201_06025"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRV98125.1"
                     /translation="MNTLKRISLGLSAVLCILLIATALPVFAEVQVFIDAHTVRVYDP
                     VLKRQSILNDFRTSGYRVHNHLALVWSEDAAWVYDVRTQQWLSESDFRTLLGSLSDEY
                     ALVWNENEAAIFDAKKQEWIRSDYIPWKLTGASLSRGMTILTGEEGLVVYDPVLKKWL
                     SSQFFPYGALKKAQAGDVLAAAWSDNDLVLYDMTIHDWVVKEGISPQACIIDGYKASI
                     FTADTVYTYDAISHRWSSKSR"
     gene            complement(1009..1794)
                     /locus_tag="P5201_06030"
     CDS             complement(1009..1794)
                     /locus_tag="P5201_06030"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRV98126.1"
                     /translation="MKKMLAVCMAVVLFFALLPSGEARAELPKGKTIAVLVKGTSHAP
                     LARSILIRALLDEGYKAVDEKQLERIRQNKAAILALEGNVEAIMALGRTFGFSVLLSG
                     SVAVPSPVRNEFGLFTATATVSATACTASNARQVAAGSASAKEIGYTADEATRKAVEV
                     ATRSAAASLLGKTPPQPPDSAGATFLVIAPARSFAEAHALAASCRDAGASNASVSRFA
                     GGRAEIDVSYSGSARQLLAALLGKRPDLKEETVEGNTLRLVGE"
     gene            complement(1800..2270)
                     /locus_tag="P5201_06035"
     CDS             complement(1800..2270)
                     /locus_tag="P5201_06035"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRV98127.1"
                     /translation="MRVCRVLVCLLVVFFSSAAFASLPAEGTEDLAALFQSIVESKSV
                     AEDIQVFRFGVVDWKGESVTSQGKAPLPSTSPQDQMLAKRGALTDARRNLLCLLYEIR
                     HGLPEKMSSIEIEGEVVDGQVDFQGVKDGVYTVEVTVPLKRFFTESRIVRADVR"
     gene            complement(2274..>3220)
                     /locus_tag="P5201_06040"
     CDS             complement(2274..>3220)
                     /locus_tag="P5201_06040"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRV98128.1"
                     /translation="AMVQGITEMKDFEVVRDTVFSRSEGLVTATENVKEEQTNDGILR
                     LTAQCTVSASTLDGVLGPAVIDALGNPRIMVLIDEVVDGERQFLSTAEGEVLKAFQKA
                     GYMLVDPGQAGAIRKGEIEAAKISGDTAVLQELARSFQSDVLIHGKAQASTATKQKVS
                     GVVLYAVNSQLQLKAIITQTAHIIGAEGSEQRVQGTSPNDGAMKAFQKLSPRMAGSLL
                     HTVAYALVSGASGSIPGRTFKVTVSDLSFAEARSLRDSLGEVRGVNGVYQRAYANRTL
                     ELDVSAETSSEEIAIALEALGVEITGITAGTVNGRKGR"
BASE COUNT          608 a         1019 c          936 g          657 t
ORIGIN      
        1 acgagcgtga tgtcacggtt ccctgcgagc gaagtggaga cgatatcgcc gaccgtcgcg
       61 ccgaggtgaa agctcgttcc ctgggcgagg aaaggctgca cggcgacgat cattgaagcc
      121 gcgaaagccc tccccggcag tgcgagcagt gtaagcagca tcagcaccga gaaagccgat
      181 atgtttcgcc tgccgttcat tgtcggagcg cctcctctcc gacgtctcgc gccgctcctc
      241 atctgctctt gctgctccat ctgtggctta tcgcgtcgta ggtgtatacc gtgtccgcgg
      301 tgaagatgga cgccttgtac ccgtcgataa tgcatgcctg aggcgaaatc ccctccttga
      361 ccacccaatc atggatcgtc atgtcgtaga gcaccaggtc gttgtccgac caagcggccg
      421 caagcacgtc gcccgcctgc gcctttttca gcgcgccgta ggggaagaac tgggacgaaa
      481 gccatttttt cagcaccgga tcatagacca caaggccttc ttcgccggtc agtatcgtca
      541 ttccgcgcga aagcgacgcg ccggtgagtt tccatggaat gtagtcgctc cggatccatt
      601 cctgcttctt cgcgtcgaag atcgcggcct cgttctcgtt ccagacgagc gcgtactcgt
      661 cggagaggga ccccagaagc gtccggaagt cgctctccga gagccactgt tgggtgcgca
      721 cgtcgtagac ccaggccgca tcctcgctcc agacgagggc aagatggttg tggacgcggt
      781 atccgctcgt acggaagtcg ttcaggatgg actggcgttt cagcaccggg tcgtacaccc
      841 gcacggtgtg tgcgtcgatg aagacctgta cttcagcgaa aaccggaagc gccgtcgcga
      901 taaggagtat gcagaggact gcggaaagtc cgagagatat ccgtttcagt gtattcatag
      961 gataccagct cccttcatgt gtgtttctga acttcttcat tgattcccct attccccgac
     1021 aagacggagc gtgtttccct cgacggtctc ctccttcaga tccggacgtt ttccgaggag
     1081 ggctgcgagg agctgcctgg ccgaaccgga ataggaaacg tcgatctccg ccctgcctcc
     1141 ggcgaaacgg gagaccgagg cgtttgaagc cccagcgtcg cggcagcttg cggcaagggc
     1201 gtgcgcctcg gcgaacgaac gcgccggagc gatcaccaaa aacgttgctc cggccgaatc
     1261 cggcggttga ggaggcgtct tgccgaggag cgacgccgct gcggagcgcg ttgcaacctc
     1321 cacggctttt cgcgtcgcct cgtccgccgt gtaccctatc tcctttgcgg aggcgcttcc
     1381 ggcggcgacc tgacgcgcgt tggaagccgt gcacgccgtc gccgacaccg tcgcggtcgc
     1441 ggtgaagaga ccgaattcgt tccgcaccgg agacgggacg gccacggagc ccgaaagaag
     1501 gacggagaag ccgaatgtcc tgcccagcgc cataatcgcc tcgacgttcc cttcgagcgc
     1561 caggatggcc gccttgttct ggcggatccg ttcgagctgt ttttcgtcga cggccttgta
     1621 cccttcatcc agaagcgccc ggatcagtat cgatctcgcg aggggggcat gcgacgtccc
     1681 cttcacaagg acggcgatag tcttgccctt cggcagttcg gcccgcgcct cgccggacgg
     1741 aagaagcgca aagaacaaaa cgacggccat gcagactgcg agcatttttt tcatggcatt
     1801 tacctcacat cggcgcgaac gattctgctc tcggtgaaga atcgcttcag cggaaccgtc
     1861 acctcgaccg tgtacacgcc gtccttcacg ccctgaaaat cgacctgtcc gtcgacgacc
     1921 tcgccctcta tctcgattga gctcatcttc tccgggagtc cgtgacgaat ctcataaagg
     1981 aggcagagaa gattgcggcg cgcatccgtc agcgcccccc gttttgccag catctggtcc
     2041 tgcggggagg ttgaaggaag aggggccttc ccctgcgacg tcacggactc gcccttccag
     2101 tcgacgacgc cgaagcgaaa gacctgaata tcttccgcaa cgctcttcga ctcgacgatg
     2161 gactgaaaga gagccgccaa atcctccgtt ccctccgcag gaagcgacgc gaaggctgcg
     2221 gaggagaaga aaacgaccag aaggcacaca agtactctgc agactctcaa cgtctatcgc
     2281 ccctttcgtc cgttcaccgt ccctgcggta ataccggtga tttctacgcc gagcgcctcc
     2341 agcgcgatgg cgatctcctc cgaagaggtt tccgccgaca catccagttc cagcgtccgg
     2401 ttcgcgtagg cgcgctggta gactccgttg acgccgcgta cctctcccag actgtctcga
     2461 agcgacctgg cctcggcgaa tgaaagatcg ctcacggtga ccttgaacgt tcttccgggg
     2521 atactgccgg aagcgccgct gacgagcgcg taggcgactg tgtgcagcag cgagccggcc
     2581 atgcgcggtg acagcttttg aaacgccttc attgcgccgt cgttcggcga cgtcccctgc
     2641 actctctgtt cggagccctc ggcgccgatg atatgggccg tctgggtgat gattgctttg
     2701 agctgaagct gcgagttgac ggcatagagc acgacgccgg aaactttctg tttcgtcgct
     2761 gtgctcgcct gcgcctttcc gtggatcagc acgtcggact gaaaactccg tgcaagctcc
     2821 tgaagcacgg cagtgtcgcc cgatattttc gcggcttcga tctctccctt gcggatcgcc
     2881 cctgcctgtc ccgggtcgac gagcatgtac ccagccttct gaaacgcttt cagtacttct
     2941 ccctccgccg tggagaggaa ctgtcgctcg ccgtcgacaa cttcgtcgat gagcaccatg
     3001 atcctcggat tgcctagagc gtcgatcaca gcggggccga gaactccgtc gagcgtcgac
     3061 gccgagacgg tgcactgcgc ggtcaggcga agaatgccgt cgttagtttg ttcttccttc
     3121 acattctccg tggctgtcac cagcccctcc gaacgggaga agaccgtgtc gcgcaccact
     3181 tcgaagtctt tcatttccgt gataccctga accattgcgc
//
LOCUS       DAOTIL010000238         3214 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminobacteriaceae bacterium isolate AMR_MDS_3026
            NODE_35509_length_3214_cov_2.800886, whole genome shotgun sequence.
ACCESSION   DAOTIL010000238 DAOTIL010000000
VERSION     DAOTIL010000238.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868050
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminobacteriaceae bacterium (wastewater metagenome)
  ORGANISM  Aminobacteriaceae bacterium
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae.
REFERENCE   1  (bases 1 to 3214)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3214)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:19:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,224
            CDSs (total)                      :: 3,198
            Genes (coding)                    :: 3,184
            CDSs (with protein)               :: 3,184
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3214
                     /organism="Aminobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_35509_length_3214_cov_2.800886"
                     /isolate="AMR_MDS_3026"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:3034609"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(21..650)
                     /locus_tag="P5201_06045"
     CDS             complement(21..650)
                     /locus_tag="P5201_06045"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRV98129.1"
                     /translation="MRSWDEIAEILSRYADKTDRSSLFLGFFSEAYQGISGKNPVLVG
                     GGAVEVYTHGGYVTGDLDLICDAEIARKILTGWGFSSAAFSRSFYRPEMDLYVELLGA
                     QDVNGDPEATSRVEAMLVGGTMLFCIISLEDLIIDRLLAFVHWGHRESALWAQAMMKG
                     AIGSEEPLDTGYIRRKLSEQEDAEKLSAAFESLLKEVLSIPEKEREGEE"
     gene            841..1419
                     /gene="clpP"
                     /locus_tag="P5201_06050"
     CDS             841..1419
                     /gene="clpP"
                     /locus_tag="P5201_06050"
                     /EC_number="3.4.21.92"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014163219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009368 - endopeptidase Clp complex
                     [Evidence IEA];
                     GO_function: GO:0004176 - ATP-dependent peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp endopeptidase proteolytic
                     subunit ClpP"
                     /protein_id="HRV98130.1"
                     /translation="MIVPHVIEQSGRGERAYDIYSRLLKDRIVFLGEGVDEHTGNIVV
                     AQLLFLESEDPKKDIHLYINSPGGYVSAGLAIYDTMQYVKCDVSTICIGQAASMGALL
                     LAGGAPGKRFSLPNSRIMIHQPLGGIQGQATEVEIHAREILKLRDRLNDIMVRHTGQP
                     KEKIAADTERDYYLSATDAMKYGIIDRVIETQ"
     gene            1436..>3214
                     /locus_tag="P5201_06055"
     CDS             1436..>3214
                     /locus_tag="P5201_06055"
                     /inference="COORDINATES: protein motif:HMM:NF019344.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HRV98131.1"
                     /translation="MKRKNENTAHSHAPGGNSLSSMPIIQSLGRRIEPEDSQGSSGIA
                     SRRDIVDEMAVVLNSCERRNIILLGKAGTGKTTLLRQLATRIEEGTAGKKLEGAVLVE
                     LDISLMKSEGCIAGGIEKKIRHMWAEAENFGNVVFFMDEGHKTMSGGSDSIGNILKPL
                     VTSGRVPVILATTDDEYVSIERDAAMKRRFSPVHVPEPDRDTLLKILAHKGAVYAKDS
                     DVAIGRGVCEKTLELAKRYIHDSADPDRSLRVLEKACSIASLEGGKRQVTAQHLLRAV
                     AMITNASALSEEEECGREQILKALEKTVKGQESALKKTADFLVRVNTGFHFKGRPKGV
                     LLLTGPAGCGKTLLAESAGAILGFKPDETVRINLSQLGCMPVQQLLGYGDFSGILTSP
                     LRKNPCRLVLLEDVDKADRDVYPVLHRMLDEGILRDALDRTADFRQSVVVMTASLENA
                     GLESRSSIGFASPTTENGDEMEEHLLHCAQKILPKTLIDRIGGFIALDPLREEHLAEI
                     FRSKFEPLLREMAKERGYLAEFSPEVVRLLASEGANRGGGAREFEKLVVERLMTPLAG
                     HIAGAPSGKTKLVIEVHDGSVVICSLA"
BASE COUNT          742 a          931 c          920 g          621 t
ORIGIN      
        1 ctcttcgtat ctgcggacct tcactcttct ccctcccttt ctttctccgg aatcgagagc
       61 acttccttca acaggctttc gaacgcggcg gacaattttt cggcatcctc ctgctccgaa
      121 agcttccggc ggatatatcc ggtgtccagg ggttcttccg atccgatcgc ccccttcatc
      181 atggcctgcg cccagagcgc gctttcccga tgaccccagt gaacaaaggc gagaaggcgg
      241 tcgatgatca ggtcttcgag cgagattatg caaaagagca tcgtcccccc gacgagcatg
      301 gcttcgaccc gagacgtcgc ctccgggtcg ccgttcacat cctgcgctcc cagcagctcc
      361 acgtagagat ccatctccgg gcgatagaat gacctcgaga atgccgcact cgaaaaaccc
      421 catccggtaa gaatctttcg cgctatctcc gcatcgcaga tcagatccag atcgccagtg
      481 acgtagcctc cgtgagtata cacttcaacc gccccgccgc cgacaagcac cggattcttt
      541 ccggagatgc cttggtacgc ctcgctgaaa aaacccagga aaagagacga ccgatccgtc
      601 ttgtcggcat agcgtgaaag gatctcggct atttcgtccc aacttctcac agaaaacacc
      661 tccttttgtt cacccttgac aggattggaa aagcgtgata ttgttgcgaa catgaataga
      721 ttgcgtttta atctcattac gctatatacc tctaattctt aaccgaatgg ctgtgctctt
      781 tatcgaaaac gcgaacagct gccggaaaca catttcaatt aaacagagga ggcgccacat
      841 atgattgtac cgcatgttat tgaacaaagc ggacgcggtg agagggcgta cgacatttac
      901 agccgtcttt tgaaggacag aatcgtcttt ctcggcgaag gcgtggacga gcatacgggg
      961 aatatcgtgg tggcgcagct gctctttctt gagagcgagg atccgaagaa ggacatccat
     1021 ctgtacatca acagcccggg agggtacgtt tccgcggggc tggcgatcta cgacaccatg
     1081 cagtacgtca agtgcgacgt ctccaccatc tgcatcggcc aggccgcaag catgggagcg
     1141 cttctactcg caggaggggc gccgggaaaa cggttctccc tgccgaactc gcgcatcatg
     1201 atccaccagc cgctgggcgg gatacagggg caggcgaccg aggtggagat ccacgcccgc
     1261 gagattctga agctccgcga ccgtctgaac gacatcatgg tcaggcacac cgggcagccg
     1321 aaggagaaga ttgccgccga cacagagcgc gactactatc tctccgcgac cgacgcgatg
     1381 aagtacggca ttatcgaccg cgtcatcgag acgcagtaga aaggtaggtt tcgccgtgaa
     1441 acggaagaat gaaaacaccg cgcattctca cgcaccgggc ggaaattcac tctcttccat
     1501 gcccatcatc cagagcctgg ggagacggat cgaacccgaa gactcccaag gttcgtcggg
     1561 catcgcctcc cgccgggata ttgtcgacga gatggcggtc gttcttaact cctgcgagcg
     1621 gaggaacatc atccttcttg gaaaggccgg cacgggcaaa accacccttt tgcgtcagct
     1681 cgcaacccgc atagaagaag gaacggcggg aaagaaactc gaaggggccg tcctcgtcga
     1741 actcgacatc agtctcatga agagcgaggg ctgcatcgca ggcggcatcg agaaaaagat
     1801 acggcatatg tgggcggagg cggagaattt cggcaacgtc gtcttcttca tggacgaggg
     1861 gcacaagacc atgtcgggcg gctccgacag catagggaac atcctgaagc ccctcgtcac
     1921 cagcggaagg gtgccggtga ttctggcgac aaccgacgac gaatacgttt ccatcgaaag
     1981 agacgccgcg atgaagcgcc gcttcagtcc ggtgcacgtg cctgagccgg atcgggacac
     2041 cctgctgaag atcctcgccc ataagggagc ggtctacgcg aaagattcgg acgttgccat
     2101 cggacggggc gtctgcgaaa aaacgctcga gctggcgaaa cgctacattc acgacagcgc
     2161 cgacccggac agaagtctca gagtgctgga aaaagcctgc tccatcgctt cgcttgaggg
     2221 cggaaagcgt caggtgacgg cgcagcatct ccttcgcgcg gtcgccatga ttaccaacgc
     2281 ttccgctctt tccgaggaag aggaatgcgg ccgtgagcag attctgaagg ctctcgaaaa
     2341 gaccgtgaag gggcaggaat ccgctcttaa gaagactgcg gacttcctcg ttcgagtgaa
     2401 taccggtttt catttcaagg gaaggccgaa gggcgttctg ctgctcaccg gccccgcagg
     2461 ctgcggcaag acgcttcttg cggaatcggc gggagcgata ctcggtttca agccggatga
     2521 aacggtgcgg atcaacctct cgcagcttgg ctgcatgcca gtgcaacagc ttctggggta
     2581 cggcgacttc tccggaatcc tcacgtcgcc tttgcgcaag aacccctgcc gcctcgtcct
     2641 gcttgaagac gtcgacaagg cggacagaga tgtgtatccg gtgctgcaca ggatgctgga
     2701 tgaagggatc ctccgcgacg cgctcgaccg gactgcggac ttccggcaaa gcgtcgtcgt
     2761 catgacggct tcattggaga acgccgggct ggaatcacgg tccagcatcg gatttgcatc
     2821 gccgacgacg gaaaacggcg acgaaatgga agaacatctg ctgcactgtg cgcaaaaaat
     2881 cctgccgaaa accctcatcg acagaatcgg cggattcatc gcgctcgatc cgctgcggga
     2941 agagcacctt gcggaaatct tccgctcgaa gttcgagccg cttctgcggg agatggcgaa
     3001 ggaacgcgga tatctcgcgg agttctcccc cgaagttgtg cgtctgcttg cctccgaggg
     3061 agcaaacaga gggggcggcg ccagagagtt tgaaaagctg gtggtggagc ggctgatgac
     3121 tcctctcgcc gggcatatcg ccggagctcc ttccggaaaa acgaagcttg taatcgaagt
     3181 gcacgatggc tcagtcgtca tatgctctct cgcg
//
LOCUS       DAOTIL010000239         3208 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminobacteriaceae bacterium isolate AMR_MDS_3026
            NODE_35578_length_3208_cov_4.689185, whole genome shotgun sequence.
ACCESSION   DAOTIL010000239 DAOTIL010000000
VERSION     DAOTIL010000239.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868050
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminobacteriaceae bacterium (wastewater metagenome)
  ORGANISM  Aminobacteriaceae bacterium
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae.
REFERENCE   1  (bases 1 to 3208)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3208)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:19:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,224
            CDSs (total)                      :: 3,198
            Genes (coding)                    :: 3,184
            CDSs (with protein)               :: 3,184
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3208
                     /organism="Aminobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_35578_length_3208_cov_4.689185"
                     /isolate="AMR_MDS_3026"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:3034609"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..970)
                     /gene="pheS"
                     /locus_tag="P5201_06060"
     CDS             complement(<1..970)
                     /gene="pheS"
                     /locus_tag="P5201_06060"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006300769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009328 - phenylalanine-tRNA ligase
                     complex [Evidence IEA];
                     GO_function: GO:0004826 - phenylalanine-tRNA ligase
                     activity [Evidence IEA];
                     GO_process: GO:0006432 - phenylalanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit alpha"
                     /protein_id="HRV98132.1"
                     /translation="MQTELESIEQRFTEQLSGIASSEELQQVKVAFLGKKGAVTSFLK
                     TLGSLSPEERPVAGQAVNSLRDRMEAILDEKKSFLEEKESDAAERAYRIDVTLPPRGR
                     SIGGIHPVAQLTQDVVEILAGLGFSVALGPEIEDDFHNFEALNIPASHPARDMQDTFY
                     FPDGKLLRTHTSPVQVRSMLRYGAPLRIVCPGKVYRRDSDPTHSPMFNQLEGLLVEKD
                     ISVADMKGCLETLMAAIFSRPLKARYRASYFPFTEPSLELDIECVECSGKNPSCRICK
                     GTGWLEVAGLGMVHPNVIRSGGIDPDVYNGFAWGIGLDRIAMLKYRL"
     assembly_gap    1043..1052
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(1329..1688)
                     /gene="rplT"
                     /locus_tag="P5201_06065"
     CDS             complement(1329..1688)
                     /gene="rplT"
                     /locus_tag="P5201_06065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014806778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L20"
                     /protein_id="HRV98133.1"
                     /translation="MPRVKGGSASDKKRKKLFSITKGYWGRKKNVYRRAREAYLHSLS
                     RAYVDRKRKKRDFRRLWITRINAATRSCGMSYSAFMNGLKKSGILINRKMLSELAIND
                     MAAFTQLTVQAKNALGK"
     gene            complement(1723..1920)
                     /gene="rpmI"
                     /locus_tag="P5201_06070"
     CDS             complement(1723..1920)
                     /gene="rpmI"
                     /locus_tag="P5201_06070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013048106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L35"
                     /protein_id="HRV98134.1"
                     /translation="MPKLKSHSGAKKRFSFTGTGKLSYRRVGRGHHLTVKGPARLRKL
                     RKTGYVDATRIESLKKLLPYN"
     gene            complement(2023..2640)
                     /gene="infC"
                     /locus_tag="P5201_06075"
     CDS             complement(2023..2640)
                     /gene="infC"
                     /locus_tag="P5201_06075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014162880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-3"
                     /protein_id="HRV98135.1"
                     /translation="MNREITSREVLIIDENGVKLGTLPTAEGIRMAEERMLDLVEVAP
                     QATPPVCRILDYGKYRYQLQKKEKDARKKQKVQTLKEMKMRPKIDEHDYDFKVRAIKS
                     FLEDGHRVKVSVFFRGREMSFLDKGEAVLARVMRDCDGLGKSEGNPRMEGRYMRIMLT
                     PVPQAQIKPTPKPAAKNDVPKAEKKPDETKVNPAPEGAESRQVSE"
     gene            complement(2855..>3208)
                     /locus_tag="P5201_06080"
     CDS             complement(2855..>3208)
                     /locus_tag="P5201_06080"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRV98136.1"
                     /translation="AMSALGLAFSGLLIPYLFVYNPDLLFVDFVLTKYVLDLSIAIIG
                     IFSLSCGIIGMLRREMEFWERALFVAAGILMVNPLRVLRISSLGFFALILAFHLFSSR
                     GANAQRSGGETKEEK"
BASE COUNT          662 a          913 c          835 g          788 t
ORIGIN      
        1 tcagccggta tttgagcatg gcgatgcggt caaggccgat tccccaggca aaaccgttgt
       61 acacatccgg atcgatacct ccggaacgga taacgttggg atggaccata ccgagccccg
      121 cgacttcgag ccaccccgtc cctttgcaga ttctgcatga agggttcttg ccggaacact
      181 cgacgcactc gatgtcgagt tcgagcgacg gctcggtgaa ggggaagtag ctcgcccggt
      241 agcgcgcttt gagggggcgg gaaaaaatcg ccgccatgag cgtttcaagg caccccttca
      301 tgtcggccac ggagatgtcc ttctctacga gcaggccttc gagctggttg aacatgggcg
      361 agtgcgtggg gtcgctgtcg cgccggtaca ccttgccggg gcagacaatt cgcaacggag
      421 ctccgtagcg gagcatcgac cgtacctgca caggcgacgt atgtgtccgc agcagttttc
      481 cgtccgggaa atagaacgtg tcctgcatat cccttgcagg gtgggacgcc gggatgttga
      541 gcgcctcgaa gttgtgaaag tcgtcctcta tctcgggacc gagggctacg gaaaaaccaa
      601 gccccgcgag aatctccacc acatcttgcg tcagctgcgc gaccgggtga attccgccga
      661 tgctcctccc cctcgggggc agagtgacgt cgatgcggta tgcccgctcc gcggcgtcgg
      721 attccttctc ctcgaggaag ctcttctttt cgtctaaaat tgcttccatt ctgtcgcgga
      781 gggagttcac cgcctgcccg gcaaccgggc gttcctccgg agaaagggaa ccaagcgttt
      841 tcaaaaagga ggtcacggcg cccttcttgc cgagaaaagc gaccttcacc tgctgaagtt
      901 cctcggaaga agcgattccc gatagttgtt ccgtaaaacg ttgttcaatg ctttccagtt
      961 cagtctgaat ggatgtcacg tgccgttctc ctccttctcc taatattccg gtcaaagcga
     1021 ttgtatcctc ctccacacag gcnnnnnnnn nnaggcctgc aacggcaaat ctcccgcatg
     1081 gacgatcgga tcgggacggg gagagcgcag cgtctttggt agaaaagaag gacagaaaca
     1141 ttatacttcg ttcgaaaatt tcacttcaga gtggattata tcaccacatc cgccgaggct
     1201 ccacaggcaa agaaaaaggg ttcccgccgc tgcgtggcgg cagaaaccct cgaaatggcc
     1261 ttttaggagg ctgcgcggtt gcgtgtagtg cgccgcacaa cctgtctcgc gcttttaaag
     1321 gcgctggtct acttgcccag tgcgttcttt gcttgaaccg tcaactgggt gaacgcagcc
     1381 atgtcgttga tcgccagctc ggaaagcatc ttcctgttga tgaggattcc cgattttttc
     1441 aggccgttca tgaaggcgct gtaggacatt ccgcaggatc tggttgcagc gttgattctt
     1501 gtgatccaca ggcggcggaa atctctcttc ttgcgttttc tgtcaacgta cgccctggaa
     1561 agggagtgaa ggtacgcctc tctcgctctc ctatacacat tctttttccg tccccagtat
     1621 cctttggtta tcgaaaaaag tttttttctc tttttatcgc ttgcgctacc acctttgacg
     1681 cggggcatcg cacgtcacct ctttcataga ataaacttgt tctcagttgt acgggagaag
     1741 cttcttcaga ctctcgatac gggttgcgtc gacataccct gttttgcgca gcttgcgaag
     1801 gcgagcggga cccttcaccg taagatgatg gcctcttccc actctccggt aggagagctt
     1861 ccccgttcct gtgaacgaaa accttttttt ggctcccgaa tgggatttaa gctttggcat
     1921 ttgagcattc ctccttcctc ggatattcat caagataaaa gcccgccgca gttcgcgaca
     1981 ggcggattga acgtcatcgc attccaagcg tcctgttcgg acttattctg aaacctgccg
     2041 gctttccgct ccctcggggg cggggttcac ttttgtttcg tccggcttct tttcagcctt
     2101 gggaacatcg tttttcgcag cgggcttggg cgtcggcttt atctgggcct gaggtacagg
     2161 agtcagcatg atccgcatat accgcccctc catgcgggga tttccctcgc tcttgccgag
     2221 tccgtcgcaa tctcgcatta cccgggcgag aactgcctct cccttgtcaa ggaaggacat
     2281 ctctcttccc ctgaagaaga cggagacctt gactctgtgt ccgtcctcaa ggaaactttt
     2341 gatggccctg accttgaagt cgtagtcgtg ctcgtcgatc ttgggccgca tcttcatttc
     2401 cttcagggtt tgcactttct gcttcttccg ggcgtccttc tcttttttct gtaactggta
     2461 ccggtattta ccgtaatcga gaattctgca tacgggaggc gttgcctgcg gcgccacctc
     2521 aacaaggtcg agcattctct cctcggccat ccgaattccc tcggccgtgg gcagggtgcc
     2581 cagtttcacg ccgttctcat cgataatcag gacttctcgt gaggtaattt cccggttcac
     2641 tcggggttct ccatcgtctt ttcgtgttac tatagccttt cacctccggt agaaatataa
     2701 aaggggccga tggaaaacca ccggccccgc atacgcgaag acttctcttc aaaccaggcg
     2761 aaatagcgct ccgcaaggtg agagggtggc ccctcttctt ctcaatattt ttaagctaag
     2821 gcattatagc acgaacaccc gaagtacgca aattctattt ttcttccttc gtctctcccc
     2881 cgcttctctg cgcattcgct cctctgcttg aaaagaggtg aaaggcgaga atgagcgcaa
     2941 agaatccgag acttgatatc ctgaggactc tcagcggatt gaccatcagg attcccgcgg
     3001 cgacgaagag cgctcgctcc cagaattcca tctcccgccg cagcattccg atgattccgc
     3061 acgagagcga gaaaatgccg atgatcgcta tggacagatc cagaacgtat ttcgtcagaa
     3121 cgaagtcgac gaacagaaga tccggattgt agacaaagag atacggaatc aacaacccgg
     3181 aaaaggccag cccaagggcg gacatcgc
//
LOCUS       DAOTIL010000240         3192 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminobacteriaceae bacterium isolate AMR_MDS_3026
            NODE_35813_length_3192_cov_3.666879, whole genome shotgun sequence.
ACCESSION   DAOTIL010000240 DAOTIL010000000
VERSION     DAOTIL010000240.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868050
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminobacteriaceae bacterium (wastewater metagenome)
  ORGANISM  Aminobacteriaceae bacterium
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae.
REFERENCE   1  (bases 1 to 3192)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3192)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:19:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,224
            CDSs (total)                      :: 3,198
            Genes (coding)                    :: 3,184
            CDSs (with protein)               :: 3,184
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3192
                     /organism="Aminobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_35813_length_3192_cov_3.666879"
                     /isolate="AMR_MDS_3026"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:3034609"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            9..209
                     /locus_tag="P5201_06085"
     CDS             9..209
                     /locus_tag="P5201_06085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006301014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HRV98137.1"
                     /translation="MAVHVNKRLCKGCGLCISVCPKNVYEISNETNQKGFTVVAAVRE
                     GDCIKCKRCEITCPDMALWIEE"
     gene            318..1292
                     /locus_tag="P5201_06090"
     CDS             318..1292
                     /locus_tag="P5201_06090"
                     /inference="COORDINATES: protein motif:HMM:NF012512.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine dehydratase"
                     /protein_id="HRV98138.1"
                     /translation="MVRIIGRDDIKTAAGRISGRVRRTPVLRGDKLDALFGAEVYFKP
                     ENLQLTGSFKIRGAMNKILSLSDEERAKGIIASSSGNHAQGVAYAAKMLGIKATLVLP
                     ENAPKSKIEGTRALGAEVVLHGFDSIQRYKKLYEIKAEKGYTLVHSFNDPELIAGQGT
                     SGLEIIEDIPDVDTVVVPLGGGGLLAGVATAIKETRSSVRIVAVEPESIQRYAESRKA
                     GKPVEVPMGATLADGLMITMTGEHTYPLIDKYVDEIVPASDEFIRKALMEIVFKSKLL
                     VEPSASVGVAAALQGSFKVKPGEKICFFLSGGNIDPEKLASFLPRETA"
     gene            1504..>3192
                     /locus_tag="P5201_06095"
     CDS             1504..>3192
                     /locus_tag="P5201_06095"
                     /inference="COORDINATES: protein motif:HMM:TIGR02123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter permease"
                     /protein_id="HRV98139.1"
                     /translation="MARDIGVTECDVPMEGEAVLRKATKECAYRTEMDPMWTKAIVVL
                     SCVFILYHLVTSRFGMPPMFKHRAIHLGFILCLVWMYYPATSRSIQKRPSFVDMGLMV
                     ASIAVCGYTVLNVDAFALRGGVALPRDYYFGAVAILLVLEACRRVAGKGLLILAVVFL
                     LYAWLGRYIPGVLSHRGYSIQRIIYQMYLTTEGIYGLPIGIAATYLVLFIVFSSFLEK
                     SGLGTLFNDVSLALGGRLVGGPAKVAVIGSALVGMISGSAATNVATSGAFTIPLMKKI
                     GYKPYFAGAIEAAASTGGQIMPPIMGTGAFIMAEFLGVPYISIAAAAVIPAVLYFAAV
                     FFQVDLRARKIGLTGLSKEELPDVKKTLLRYGHMFLPIIILVVLLMQHFTPLYAAFYS
                     ILVTWLLSFLRKETRMGVGKLVPMAVASARSCLSLSVAMANAGFVVAVLSMAGIGIIL
                     ADNIVALSHGSLFITLVLCMVVSIILGMGLPTSACYIIAATIAVPILHQLNVPGIQAH
                     FFVFYFACLSTVTPPVALASYVAAGMSGAGTNEVGWAAFKLALAGFIVPFFFVYA"
BASE COUNT          643 a          910 c          940 g          699 t
ORIGIN      
        1 gagtgaagat ggccgttcat gtgaacaagc gtctctgcaa agggtgcgga ctgtgcatct
       61 cggtctgccc gaagaacgtc tacgagatat ccaacgagac gaaccagaag gggttcaccg
      121 tcgtggctgc ggttcgcgag ggagactgca tcaagtgtaa acggtgcgag atcacctgcc
      181 ccgacatggc gctgtggata gaggaataga atagcggtac gagaacgggg cggcgcggga
      241 agcttcttcc ggccgtatcc gggcttttga aggagtgcat ttcgcccctc gggggcgcaa
      301 ggaggcggaa agggaatatg gtacgaatca tcggacgtga cgacattaaa acagctgccg
      361 ggaggatctc cgggagagtt cggcggacgc cggttctgcg gggagataaa ctcgacgcgc
      421 tcttcggcgc ggaggtctac ttcaaaccgg agaaccttca gcttacaggg tccttcaaga
      481 tcaggggcgc gatgaacaaa atcctctccc tctccgacga ggaacgggca aaaggtatta
      541 tcgcatcgtc ctcggggaac cacgcgcagg gagtcgccta tgcggcgaag atgctcggca
      601 tcaaggcgac gctcgtgctc ccggagaacg cgccgaaatc aaagatcgag gggacgagag
      661 cgcttggagc cgaggtggtg ctccacggtt tcgactccat ccagcgatac aagaagctct
      721 atgagatcaa ggccgaaaag gggtacacgc tggtccactc cttcaacgat cccgagctga
      781 ttgccgggca gggaacgtcc gggctggaga tcatcgagga tattccggat gtcgataccg
      841 tggtcgtacc gctcggtggg ggcggcctgc tcgcgggtgt ggctacggcc atcaaggaga
      901 cccgttcctc cgtacggatc gtcgctgttg aaccggaatc tatccagcgg tacgccgaga
      961 gccgcaaggc ggggaagccc gtagaagtcc ccatgggggc gacgctcgcg gacggactta
     1021 tgatcacgat gaccggagag cacacgtatc cgctgatcga caagtacgtg gatgagatcg
     1081 ttcccgcgtc ggacgagttt atccggaagg cgctcatgga gatcgtcttc aaaagcaaac
     1141 tgctggtcga gccctccgct tcggtcggcg tggctgcggc gcttcagggt tccttcaagg
     1201 tcaaacccgg cgagaagata tgcttctttc tctccggcgg caacatcgac ccggagaagc
     1261 ttgcatcctt tctaccacga gagacggcgt aatattcagg ggcggcgaag cggaacgagt
     1321 cagccatgaa aacgcacccc ttcccgccgc ttttccgcca gtgtttgcca gacgtatcgt
     1381 aaagagagtt cactgtacca ctcaagaata cagtatgcac tttgcaatgc ctttgagctt
     1441 catatacaat atataatgca taaagcatga acgagtaaat atttcaggag tggagggaaa
     1501 tgaatggcgc gagatatcgg tgtcaccgag tgtgatgtcc ccatggaagg ggaagccgtt
     1561 ctgagaaagg ccacgaagga atgcgcgtac aggacggaga tggacccgat gtggacgaag
     1621 gcgatcgttg tcctgtcatg cgtgtttata ctctatcatc ttgtcacctc caggttcggg
     1681 atgcctccca tgttcaagca cagggcgatt catctcggat tcattctctg tctggtgtgg
     1741 atgtactacc ccgcgacgag ccgctccatc cagaagagac cgagtttcgt cgacatgggg
     1801 ctgatggtcg cctccatcgc cgtctgcggc tacacggtgc tcaacgttga cgccttcgcg
     1861 ctcagaggcg gcgttgcgct tcccagggac tactacttcg gcgcggtggc gatactgctc
     1921 gtccttgagg cctgccgcag agttgcaggg aaaggactcc tcattctggc cgttgtcttc
     1981 ctgctgtacg cctggctcgg ccggtacatt cccggcgttc tctcccaccg gggctactcg
     2041 atccaacgca tcatctacca gatgtacctc accacggaag gtatctatgg actccccatc
     2101 ggcattgccg cgacatacct tgtcctcttc atcgtcttct cctctttcct tgagaaaagc
     2161 ggactcggaa cgctcttcaa cgacgtctcc ctcgcgctcg gaggccgtct ggtcggcggg
     2221 cccgcaaagg ttgccgttat cggaagcgct cttgtcggta tgatcagcgg cagcgcggcc
     2281 accaacgtgg caacgtcggg cgccttcacc atcccgctga tgaaaaaaat cgggtataag
     2341 ccttattttg ccggagctat tgaagcggca gcctcaacag gagggcagat catgccgccg
     2401 atcatgggga ccggagcgtt catcatggcg gagtttctcg gagttcctta catttcgata
     2461 gcggcggcgg cggttattcc ggcggtcctc tattttgcag cagtcttctt ccaggtagac
     2521 ctccgggcgc gcaagatcgg tttgacgggg ctttccaagg aggaactgcc cgacgtgaaa
     2581 aagacgctcc tccggtacgg gcacatgttc ctgccgatca tcatcctcgt cgttctgctc
     2641 atgcagcact tcacaccgct ctatgcggca ttctactcga tcctggtcac ctggctgctc
     2701 tcgttcctgc gaaaggaaac gcgcatggga gtcggcaaac tcgtccccat ggcggttgct
     2761 tcggctcgtt cctgtctgag tctcagtgtt gcgatggcga acgccgggtt cgttgtcgcc
     2821 gtgctttcga tggccggtat cggcattatc cttgccgaca atatcgttgc cctgtcgcac
     2881 ggcagcctct tcattaccct ggtactctgc atggtcgtct caatcattct tggtatggga
     2941 ttgcccacca gcgcgtgcta cattattgcg gcgaccatcg ccgtgccgat acttcaccag
     3001 ttgaacgtcc ccggaataca ggcgcacttc ttcgtctttt acttcgcatg tctctcgacg
     3061 gtaacgcctc ctgttgccct ggcctcctac gttgccgcgg ggatgtcggg cgccgggacg
     3121 aacgaggtcg gctgggcagc cttcaaactt gcgctcgccg gcttcattgt cccgttcttc
     3181 ttcgtctacg cc
//
LOCUS       DAOTIL010000241         3179 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminobacteriaceae bacterium isolate AMR_MDS_3026
            NODE_36002_length_3179_cov_3.350512, whole genome shotgun sequence.
ACCESSION   DAOTIL010000241 DAOTIL010000000
VERSION     DAOTIL010000241.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868050
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminobacteriaceae bacterium (wastewater metagenome)
  ORGANISM  Aminobacteriaceae bacterium
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae.
REFERENCE   1  (bases 1 to 3179)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3179)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:19:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,224
            CDSs (total)                      :: 3,198
            Genes (coding)                    :: 3,184
            CDSs (with protein)               :: 3,184
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3179
                     /organism="Aminobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_36002_length_3179_cov_3.350512"
                     /isolate="AMR_MDS_3026"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:3034609"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(374..2434)
                     /locus_tag="P5201_06100"
     CDS             complement(374..2434)
                     /locus_tag="P5201_06100"
                     /inference="COORDINATES: protein motif:HMM:NF026177.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HRV98140.1"
                     /translation="MKNRKGIFSSRRLSLMAKLGGGTALLVIVVVAITAGASLSRFSE
                     VLEENAHQKSILAAHGLESILEAKTAESRMATLFLVSAPGLADTVKAGNSKALAEQLT
                     PLWKEQTDLDFAAVLDPKGTVLLRLHEPDKNGDSLADEFNVRGAMKGQTTSAIEPGTQ
                     VPLSARTATPIKDASGALVGILTTGISFSREAFVDQAKELFDAEVTVFAGDTRLMTTI
                     RRDGERVVGTKLDPAIAAVVLSGKSYYGAAEILGAPYITAYEPILGPDGKPVGIYFSG
                     ISMAALANVQQSTFWVALGVAAVGLLFLGLLVRKIVKSIRTMVFFMKEVEAGRLYHTR
                     EEFGVTSHDEIGDMADALAEMVRVQREMISELKEKSVHLSALSEETAASTEEVTTTTN
                     EVAESNSKLAEQTRNGRLNAVESSKVMLEMSSLIQIAQTLASSADKNSGGMSEAVLEG
                     RETVFRTIERIEGVKGAVEETENVLSQLDAYSQRIGVVGDTITGIADQTNLLALNAAI
                     EAARAGEAGRGFAVVAEEVRKLAEQSQQGAREVSELVGKILDGTNLAVASMRKSREGV
                     EEGVSVAHVAGSALERIKEAIDKSIADIRRIIDTTNEEVARSDKVIKLIDTTASVMEN
                     TDDYVQNLAASMEETAAAMENVATSANEVSESSEELRRMTDRFQVEKDGGGTTSAIVP
                     KRGA"
     gene            2877..>3179
                     /locus_tag="P5201_06105"
     CDS             2877..>3179
                     /locus_tag="P5201_06105"
                     /inference="COORDINATES: protein motif:HMM:NF024811.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD hydrolase-like protein"
                     /protein_id="HRV98141.1"
                     /translation="MIATPIAFLAGETELRREKPELVKAVIFDLDGTLIDSEENHYES
                     DRVLLARRGVTFSREDKKAFVGKDITEMVRRIKANYGLASDAQSLMDEKNAIYRSLA"
BASE COUNT          647 a          958 c          840 g          734 t
ORIGIN      
        1 gaggtaattc gagaagatca ctacggcgga accagccgtt accgccacca gaagcagcgc
       61 caaaagcgcc gtgcctccgc cgagtttcgc catcagtgag aggcgcctgc cgtgcttcgt
      121 cctttccgct ataccacgtt cattcgcttt cattgctttt cctccgcctt tccagcttga
      181 gatatctgtt ctttactaag actacatcca ttcagaggca attattcccc ttgaacaaat
      241 gtattgacga agagtcattc gactccgcca aataatcgga cagcgcgcgt gccaaaaaaa
      301 gccggagcgt tccctttgcg gcgaacaccc cggcatctcg ttctttctca ggacgggact
      361 ctccggaggc cgctcaggct cctctcttcg ggacgatcgc ggacgtcgtt cctccgccgt
      421 ctttttcgac ctggaaacgg tcggtcatcc gcctgagctc ctcggaggat tcgctgactt
      481 cgttcgcgct cgtcgcgacg ttctccatcg cggcggccgt ctcctccatc gaggccgcga
      541 ggttctggac gtagtcgtcg gtgttctcca tcacgctcgc cgtcgtgtcg atgagcttga
      601 taactttgtc cgatcgagcg acctcttcgt tggtggtgtc gataatccgg cggatatccg
      661 ctatcgattt atcgatggcc tccttgatgc gctcaagagc gctccccgca acgtgcgcga
      721 cggaaacgcc ctcctcgact ccctcgcggc tcttgcgcat cgacgctacc gccaggttcg
      781 taccgtcgag gatctttccg acgagttccg acacctcccg agcgccctgc tgcgactgct
      841 cggcgagttt gcgtacttct tcggcgacga cggcgaatcc gcgcccggct tcccctgccc
      901 gcgcagcctc tatcgcggcg ttgagcgcca gcagattcgt ctggtcggcg ataccggtga
      961 tggtgtcgcc gaccacgccg atccgctgcg agtaggcgtc gagctgcgag agcacgtttt
     1021 cggtctcttc aaccgccccc ttgactccct cgatgcgttc gatggttctg aacaccgtct
     1081 cccggccttc gagcacagcc tccgacattc ctccggagtt cttgtccgcg ctggacgcaa
     1141 gggtctgcgc tatctggata aggctgctca tctccagcat gactttcgag gattcaacgg
     1201 cgttgagccg tccgttcctc gtctgctcgg ccagcttgga attgctctcg gcaacttcgt
     1261 tggtcgtggt ggtcacttcc tcggttgagg cagcggtctc ctccgaaagg gctgaaagat
     1321 ggacggactt ctccttcagt tcgctgatca tctcccgctg cacgcggacc atttcggcaa
     1381 gagcgtcggc catgtcgcca atctcgtcgt gagaggtcac gccgaactcc tcccttgtat
     1441 ggtagaggcg ccccgcttcg acctccttca tgaagaagac cattgtccgg atgctcttta
     1501 cgatcttccg gaccagcaac ccaaggaaga gcaaaccgac tgccgccacc cccagcgcta
     1561 cccagaaagt cgactgctgc acattggcca gcgccgccat ggaaataccg gagaagtaaa
     1621 tgcccacagg cttgccgtcg ggtccaagga taggttcgta cgcagtgatg tagggggcgc
     1681 cgagtatttc agccgcgccg taataactct tccccgagag cacaacggct gctatcgccg
     1741 ggtcgagctt cgtcccgaca acacgctccc cgtcgcggcg aatggtggtc atcagacggg
     1801 tatccccggc aaagacggta acctccgcat cgaagagctc cttcgcctgg tcgacgaacg
     1861 cctctcttga aaatgaaatg cctgtggtga gaatgcccac gagcgctccg gaggcgtcct
     1921 tgatcggggt cgcggttcgc gccgagaggg gtacttgcgt tccgggttct atggcgctgg
     1981 tcgtttgtcc tttcatggcg cccctaacgt tgaactcatc ggccagcgaa tcgccgtttt
     2041 tgtcgggttc gtgaagacgg aggagcaccg tgcccttcgg atcgagcacc gccgcgaagt
     2101 caagatcggt ctgctctttc cacaagggcg tcagctgttc ggcgagagcc ttggaattcc
     2161 ccgccttcac cgtgtcggca agccccgggg cggagacaag aaagagcgtc gccatccttg
     2221 attccgcagt tttcgcctcg agaatactct ccagtccgtg cgcggcgaga atggacttct
     2281 ggtgcgcgtt ctcctccagg acctccgaga aacggctgag cgaggcgccc gcagtaatgg
     2341 ccacaacaac aatgaccagc agggccgttc ctccgcccag cttcgccata agagagagac
     2401 gccgcgacga gaagattcct tttctattct tcactttttc ccctccgtga aatgaaaatt
     2461 taaaagccag cttatacatt gtaacatctt cttctcgaaa gacaattcat tacagcggat
     2521 tatataaatg agctttaatg tgtttgttaa ttttctatag ttccatttat aaataaattt
     2581 ataacgaact cagaaaagcg cacacttgaa agtaacaaaa taatacgact tggtttctca
     2641 tctcgaagaa accttaaaga aaaggaatat ccttccattc tctttatcgg ctcttgctat
     2701 cgactatttc aattgcatct tcgtttgcat actatttttc ggtttttccg ccgcactgcg
     2761 gcggacggag gcgcaacaac atgcagagtt gaattcaggt ggagatcgta ttttcctgaa
     2821 agaaattcca tgttacaatt cttccggacc agagcattcg ggaagggaga atcacaatga
     2881 tcgcgacacc aattgcattt ctcgcgggcg aaacggaact gcgccgggaa aaaccggagc
     2941 ttgttaaggc ggttatcttc gatctcgacg gcactctgat cgacagcgag gagaaccatt
     3001 acgagagcga ccgggtgctc cttgcgcgcc ggggcgtcac tttttcacgg gaggacaaga
     3061 aggccttcgt cgggaaggac atcaccgaga tggtccggcg gatcaaggcc aactacggcc
     3121 ttgcgagcga cgcgcaaagc ctgatggacg agaagaacgc aatctaccgc tcgcttgcg
//
LOCUS       DAOTIL010000242         3175 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminobacteriaceae bacterium isolate AMR_MDS_3026
            NODE_36061_length_3175_cov_3.409295, whole genome shotgun sequence.
ACCESSION   DAOTIL010000242 DAOTIL010000000
VERSION     DAOTIL010000242.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868050
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminobacteriaceae bacterium (wastewater metagenome)
  ORGANISM  Aminobacteriaceae bacterium
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae.
REFERENCE   1  (bases 1 to 3175)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3175)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:19:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,224
            CDSs (total)                      :: 3,198
            Genes (coding)                    :: 3,184
            CDSs (with protein)               :: 3,184
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3175
                     /organism="Aminobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_36061_length_3175_cov_3.409295"
                     /isolate="AMR_MDS_3026"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:3034609"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..574
                     /locus_tag="P5201_06110"
     CDS             <1..574
                     /locus_tag="P5201_06110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005658566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HRV98142.1"
                     /translation="TESLRAFCESLDISYSVKTHPIVNIINIRNERSPCSLCANIRRG
                     ILNAAVNESGCNVLALGHNLDDAVETALMNLFHTGRFKSFQPKFWQSRSGVTVIRPLI
                     LVEERRILKEVSRLELPVLPYICPFSLETERARAKKIIDQLAAGNPRIKYNIIHALKS
                     IDEGDRWELSAHRKKQEHSPGSGSFVPILD"
     gene            664..2502
                     /locus_tag="P5201_06115"
     CDS             664..2502
                     /locus_tag="P5201_06115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012869546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PEP/pyruvate-binding domain-containing protein"
                     /protein_id="HRV98143.1"
                     /translation="MGKNEEYSVQLPPSGQFSPSEKSASFTENEEIVRRYFRWEPLDD
                     PSFAHLIVGKGSIGGKGRSLLFAMAKLRDSGVETLKRVVFPPSLFIATDSFDSVVSQI
                     PNLEALKKGSPETIEEAFLATKLPDSVLGAIRKFLEKITDPIVVRSSSVLEDSLKYSF
                     AGKYLSTFEFNGGDESLEERAAHVESDIKKIYARTFFPVAVAYRQKHGLGDDRMGIIL
                     MRVAGKWRGRYYYPTMAGVGFSRYYRRWTTRIKSEDGIIRLVFGMGTTSTKRGYARTF
                     ALTLPSLRPEGQNPFNIMRHSQEHFQVIDREQKALVTVDVKQIWKDIFPYHSCFPDYA
                     QIYSPDEEGGYFTPMSRHGSVIEKGEKVCFTFEEFAFRSKHFFERMKQLLSLLDREMG
                     VPADVEFAFHPTEDLVEIVQARPLWISEQTSTATIPPLENRNVVLRADRMVTHGVKEN
                     IPYLVYIDHHVYAQETRFHDVARAVGSVNDLLKGSRFVLVAPGRVGSSNPLLGVPVQY
                     NEITRCCCIVEVGFPKEGYMPELSFGTHFFTDLEIDGILYMPVYEGAKNNIFDEHFFD
                     SSPYTLGGHAGVRIYSGSFSVYTDGDKNLGVVVADRVDEPESGWAE"
     gene            complement(2568..>3175)
                     /locus_tag="P5201_06120"
     CDS             complement(2568..>3175)
                     /locus_tag="P5201_06120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006583314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="glucose-6-phosphate isomerase"
                     /protein_id="HRV98144.1"
                     /translation="SSASSYGNMTVMMPYEDALERFCEWFAQLWGESLGKNGVGSTPV
                     RALGAIDQHSQIQLYTEGPDDKLFSIITVSEPEEDIVIPETEEQALVPLGYLFGQSMN
                     SLRRYEALSTAAAVAKGGKPVVTIEVPRLDERRLGALIQLYEHVTALTGYVLDVNPFD
                     QPGVEQGKNYTYGLMGRPNFSAQALEVTELAEKLSLMEVTL"
BASE COUNT          739 a          885 c          851 g          700 t
ORIGIN      
        1 tacagaatct ttgcgagcgt tctgtgaatc cctcgacatt tcgtactcgg taaaaacaca
       61 tcccattgtc aatattatca atatcagaaa cgagcgttca ccttgcagcc tgtgcgcgaa
      121 catccgcaga ggaattttga acgcggcggt gaacgagtcc gggtgtaatg tgctcgctct
      181 gggtcacaac ctggacgatg ccgtcgaaac tgcacttatg aatctcttcc acaccggcag
      241 attcaagtct tttcagccga aattctggca aagccgctcc ggcgtcacgg tcatccgccc
      301 gctcatcctg gtagaagagc ggcgaatctt gaaggaagtc tcacggctgg aactcccggt
      361 tcttccctac atctgcccct tttctctgga gaccgaaaga gcgcgggcaa aaaaaatcat
      421 cgatcaactt gctgccggaa atccaagaat caagtacaat ataatccacg cactcaaatc
      481 gatcgacgaa ggagaccgat gggaactttc tgctcatcgg aagaaacagg aacattctcc
      541 cggctccggt tcttttgttc cgattcttga ttaaacaggt cttcagcctc cggcatgtcg
      601 aaaccggatg aacctggtcg ctcgcgtctc aaggagcgac aatgctcttc aaggagagat
      661 gacatgggaa aaaatgaaga atactcggta cagcttccgc catcgggaca gttctcccca
      721 tcagagaaga gcgcgtcgtt tacggagaac gaagaaattg tcaggagata cttccggtgg
      781 gagcctttgg acgatccttc gttcgcacac ctgatcgtcg gcaagggcag tatcggcggt
      841 aaagggcgat cccttctttt tgcaatggcc aaactccggg acagcggcgt tgagacgctg
      901 aagcgggtgg tctttccgcc gtctcttttt attgccactg attctttcga cagcgtggtc
      961 tcgcagatcc cgaaccttga agcactgaag aaaggatcgc ctgaaactat cgaagaagcg
     1021 tttctggcga cgaagcttcc tgacagcgta ctcggcgcga tcagaaaatt cctcgaaaaa
     1081 atcaccgatc ctatcgttgt tcgcagcagc agcgtcctgg aggattctct caaatactcc
     1141 ttcgccggga aatacctctc gacgttcgaa ttcaacggag gggacgaatc tctcgaagaa
     1201 cgagccgctc atgtagaaag cgacatcaaa aaaatttacg cgcgcacgtt tttcccggtt
     1261 gcggtcgcct atcgccaaaa gcacggactg ggcgacgaca gaatgggaat tatcctgatg
     1321 cgcgtcgccg gcaagtggcg cggccgctac tactacccga ccatggcggg cgttggtttc
     1381 tcccggtact accgacgatg gaccacgagg attaaatccg aagacggcat catccgcctc
     1441 gtcttcggca tgggaaccac cagcacgaaa cgagggtacg cccgtacgtt cgcactgacg
     1501 ctcccttcgc tccgtccgga gggtcagaat cctttcaaca tcatgcggca ctcccaggaa
     1561 cacttccagg tgatagacag agaacagaag gcgcttgtca cagtggacgt caaacagata
     1621 tggaaggata tcttccctta ccacagctgt ttccccgact acgcgcagat ctacagcccg
     1681 gatgaggagg gaggctactt cacaccgatg tcccgtcacg gctcggtcat cgaaaaggga
     1741 gaaaaggtct gtttcacttt tgaagaattc gcattccgtt caaaacactt tttcgagagg
     1801 atgaaacagt tgctctctct tcttgacagg gagatgggtg ttcctgccga cgtcgagttc
     1861 gcgttccacc cgaccgagga tcttgtggag atcgtccagg cccgccccct ctggatcagc
     1921 gaacagacgt ctacggcgac catacctcct cttgaaaacc gcaatgtcgt gctgcgcgcg
     1981 gatcgaatgg ttacgcacgg cgtaaaggaa aacatccctt atcttgtcta tatcgaccat
     2041 cacgtctacg cgcaggagac gcgatttcac gacgtcgcga gggcggttgg ttctgtgaac
     2101 gatttgttga aaggctcgag attcgtcctt gtggcgccgg gacgcgtcgg ttcgagcaac
     2161 ccgctgctgg gagttcccgt ccagtacaac gagattaccc gctgctgctg catcgtagag
     2221 gtagggtttc caaaggaggg gtacatgccc gaactctcgt tcggcacgca cttcttcacc
     2281 gacctggaga tagacggtat tctgtacatg ccggtgtacg agggcgcaaa gaacaacatc
     2341 ttcgacgaac acttcttcga ttcctctccc tatactcttg gcggacacgc aggagtgcgt
     2401 atctactcgg gctccttttc ggtgtacacc gacggagaca aaaatttggg cgtcgttgtg
     2461 gcagacaggg tggacgaacc cgagtcaggg tgggcggaat agttttcgat actgcgaagg
     2521 ccgcgcggag agttgctcct gcgcggcctt ctttttttca gaaaaactca gagggtgact
     2581 tccataagag aaagtttctc ggcaagctcc gtcacttcaa gagcttgggc gctgaaatta
     2641 ggccgcccca tcagtccgta ggtgtaattc ttcccctgct ccaccccggg ctggtcgaac
     2701 gggttcacat caagaacata tccggtgagc gctgtaacat gttcgtagag ttgaatcagg
     2761 gcgccgagcc gccgttcatc caagcgcggg acctctatgg tcacgaccgg tttccctcct
     2821 ttggcgacag cagcggcagt cgaaagcgct tcgtaccggc gcagcgaatt catcgactgg
     2881 ccaaaaaggt atccgagcgg gacgagcgct tgctcctcgg tctcggggat cacgatatcc
     2941 tcctccggtt cggacacagt gatgatggaa aagagcttgt cgtcgggccc ttcagtgtac
     3001 agctgaatct gcgagtgctg atcaatggcg cccagagcgc ggacgggggt cgacccgacg
     3061 ccgttctttc ccaggctctc gccccagagc tgggcgaacc actcgcaaaa gcgttcgagc
     3121 gcgtcttcgt acggcatcat gaccgtcatg ttgccgtacg aagacgcgct cgaac
//
LOCUS       DAOTIL010000243         3173 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminobacteriaceae bacterium isolate AMR_MDS_3026
            NODE_36090_length_3173_cov_4.104233, whole genome shotgun sequence.
ACCESSION   DAOTIL010000243 DAOTIL010000000
VERSION     DAOTIL010000243.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868050
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminobacteriaceae bacterium (wastewater metagenome)
  ORGANISM  Aminobacteriaceae bacterium
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae.
REFERENCE   1  (bases 1 to 3173)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3173)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:19:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,224
            CDSs (total)                      :: 3,198
            Genes (coding)                    :: 3,184
            CDSs (with protein)               :: 3,184
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3173
                     /organism="Aminobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_36090_length_3173_cov_4.104233"
                     /isolate="AMR_MDS_3026"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:3034609"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1384
                     /locus_tag="P5201_06125"
     CDS             <1..1384
                     /locus_tag="P5201_06125"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HRV98145.1"
                     /translation="DTESMEWLEGWLSVFPGTIIAVSHDRRFLDTMCTSVAELSLGSL
                     SLYQGNFSAYVEERERRIDDLRKAQKQQKEEIAHIEEFIERFRYKASKASSVQSRIKR
                     LEKIELVEIEEETKRVHFRFPPCTRSGLESIVLKDVEKRYGDKIVFHDASLSIQRGEK
                     IALVGVNGAGKSTLSRILGGTEQPSSGSVRFGHNVKLAFFSQESSQNLDYSRTVWDTI
                     SSRNAAWSEREKRSLLGAFLFGGDDIYKPVSVLSGGEKSRLALLKLLLEEANVLILDE
                     PTNHLDMQTKDLFQRALLEYDGTLVIVSHDRFFLDNLVDKVIEITSGSLIVYPGNYSW
                     FIEKRRERLASEASLLSFGNANASSEKSSQDGIRNLKRMEAEKRNALYRRKKRVLDTM
                     EPLEARIEHLERDQAQRDAQLCDPSFLADSVKVSAILIERNEAAREIADLLAQWEELA
                     EEVDRIERTG"
     gene            1636..2271
                     /locus_tag="P5201_06130"
     CDS             1636..2271
                     /locus_tag="P5201_06130"
                     /inference="COORDINATES: protein motif:HMM:NF022655.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LmeA family phospholipid-binding protein"
                     /protein_id="HRV98146.1"
                     /translation="MILENEPNDEGLIRELYLDVTGCDIGGVRIDSLKLRAVGVTLSP
                     PSQWEQEGGLDAKEVLNVHAFASITEKDLNENLLQKQFGDDDEWHNLQVRMTPDGIYA
                     RGNYHAQVLFTLNILIEIFSKFKIVDMQQVWLDDYTLRVNRVDVPDFITEKAVSQIQP
                     LLDLSKFVFPLRLSSITFKEGSMTIASRLLPEPFEGIVYTYEKNKKTEEKK"
     gene            complement(2324..2803)
                     /locus_tag="P5201_06135"
     CDS             complement(2324..2803)
                     /locus_tag="P5201_06135"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRV98147.1"
                     /translation="MDTPGHAAHHQSFLFDDDGVLTLFAGEAAGVYLEGEEPFPYLRP
                     ATPPRFFPDVTLASLDRLLERGCSRICYGHYGAAEGAREMLTFAKEQLLFWKDVILGL
                     LRNTADPRNEEFFLEELLERDPFLAAWRRMDHDIRERERMFLCNSIRGYLGAFSREK"
     gene            complement(2830..>3173)
                     /locus_tag="P5201_06140"
     CDS             complement(2830..>3173)
                     /locus_tag="P5201_06140"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRV98148.1"
                     /translation="EIDTDQLTENAQKYAHQIITSITTLTDKEREILYDSFSIAVTDA
                     STQFQKMSVRFSGLTSVECGFDNVLGRVQLDGSKIPIHQLSGRAAGKRLVKRLCSTVS
                     RLLFQRRRSFR"
BASE COUNT          824 a          804 c          809 g          736 t
ORIGIN      
        1 tgataccgaa agcatggagt ggctggaagg gtggctttca gtgtttccgg ggacgattat
       61 cgccgtatct cacgataggc gtttcttaga tacgatgtgt acttcggtcg cggagctttc
      121 actgggatcc ctttcactgt atcaggggaa tttttccgcg tacgtggaag agagggaacg
      181 tcgcatcgac gatctccgca aggcccagaa acaacaaaaa gaagagattg cacacatcga
      241 ggaattcatt gagcgattcc gttataaggc ctccaaggcg tcttcggtac agagcaggat
      301 caagcgtctt gagaagattg agcttgtcga aatagaagaa gagacaaagc gtgtccattt
      361 ccgctttccg ccgtgtacgc gaagcggact tgaatcgatt gtcctcaagg atgtcgaaaa
      421 acgatatggc gacaagatcg tttttcatga tgcttctctc tccattcaaa gaggggagaa
      481 aattgctctg gtcggggtga acggcgcagg gaagtcgacg ctctcacgga tcctgggcgg
      541 gacggaacaa ccttcctctg gctcggttcg tttcggccat aacgtcaaac tcgcattctt
      601 ctcgcaagag tcctctcaaa atctggacta ctcaaggact gtctgggata ctatctcttc
      661 tcggaacgca gcgtggtcgg aacgtgaaaa gaggagcctt ctcggggcgt tcctcttcgg
      721 aggcgacgat atttataagc ccgtttctgt tttgtctgga ggggaaaaat ccagacttgc
      781 tctcctgaag cttcttctgg aggaggcgaa cgtactgatc ctcgacgagc ccacgaacca
      841 tctggatatg cagacgaaag acctcttcca gagggcgctt ctggagtacg acggcacgtt
      901 ggtcattgtt tcgcacgacc gtttcttcct ggataattta gtagacaaag taatcgaaat
      961 cacttccggt tctttgatcg tctaccccgg gaactactcc tggttcatcg aaaaaagaag
     1021 ggaacgtctt gcttcggaag cttctcttct ttcctttgga aacgcaaacg catcatccga
     1081 gaaatcgtct caggacggaa tacgaaacct taaacggatg gaggctgaga aacgaaacgc
     1141 gctctacagg cgcaaaaaaa gggttctgga cacaatggaa cctcttgaag cgcgtatcga
     1201 acaccttgaa agggaccagg cccaacgcga tgctcagctc tgcgatcctt cgttccttgc
     1261 agattcagtc aaggtttcag cgattcttat cgaacgaaat gaagccgccc gcgagatagc
     1321 agaccttctc gctcaatggg aggaattggc ggaagaggtg gatcgtatcg agagaacggg
     1381 gtaaaacgcc gatataccaa gagaccttca aaaaatgcac tgcatatctc gtgaggagga
     1441 cacatgatgc gtattttttt aattccgtac agtcgcggca ataccagaga aagggccttt
     1501 ctccttctct ttgtttttag ccttgcactg ttcatgctat cgcaggcaaa cacagcttcc
     1561 gctgaaacgg atcaaacgcg ggggggaaaa ctgctcctgc atttcgtaga ggagttcaaa
     1621 cctgaaaaaa tacacatgat cctggaaaat gaacctaacg acgaaggcct tatccgggaa
     1681 ttgtatctcg atgtcacggg ctgcgacatc ggcggggtcc gtatagattc tctgaaacta
     1741 cgcgcagtcg gcgtaacgct ctcccctcct tctcaatggg aacaagaggg cgggctcgac
     1801 gcaaaagaag tactcaatgt ccatgcgttc gcctccatta cggagaaaga cttgaacgaa
     1861 aatttgcttc aaaaacagtt cggcgacgat gacgaatggc ataaccttca ggttcggatg
     1921 accccggacg ggatatacgc gcgcggtaat taccacgccc aggtactgtt tacgctcaat
     1981 atcctcatcg agatattcag taagttcaag atcgtcgaca tgcaacaggt gtggcttgac
     2041 gactatacgc tccgggtgaa ccgtgtcgac gttccggact tcatcacgga aaaagcagtc
     2101 tctcaaattc aaccgctcct cgacctgagt aagttcgtct ttcctttgcg cctctcttcc
     2161 attaccttca aggaggggag catgacgatc gccagccgtc ttctaccgga acctttcgaa
     2221 ggaatcgtct acacctacga gaaaaacaaa aagactgagg agaaaaagta aacgcacgct
     2281 gaatacaaag cggggaggtt ccgaacggag ccctccccgt tatttatttc tcccgtgaaa
     2341 acgccccaag gtaccctctt atgctattgc ataaaaacat acgctcccgc tctcgtatat
     2401 cgtgatccat acgccgccag gcggcaagga aagggtcccg ttccaggagt tcttccagga
     2461 aaaactcttc gtttctcgga tccgctgtgt ttcgaaggag cccgaggata acgtccttcc
     2521 agaacaacag ctgctctttg gcgaatgtga gcatctctct cgcaccttcc gccgcgccgt
     2581 aatgtccgta gcagattcgg gagcatcctc gctccagaag ccggtcaagg gaagcaagcg
     2641 tgacatcggg gaaaaaacgg ggcggtgtcg ccggccgaag ataggggaag ggttcttccc
     2701 cttcaagata gactccggcg gcttctccgg caaagagagt cagcaccccg tcgtcatcga
     2761 aaaggaaact ctgatggtgc gccgcatgac ctggagtatc cacagcgaaa aaccctttcg
     2821 gcagagaatc tatcgaaagg atcttctccg ctggaacagg aggcggctca ccgtagagca
     2881 aagccgtttc accaagcgtt tgcctgctgc ccttccagag agctgatgga taggaatttt
     2941 cgacccatcc agctgcactc tccccagtac gttatcaaac ccgcattcaa cagaagtaag
     3001 tccactgaat cgaacagaca tcttctgaaa ttgagtagaa gcatctgtca ctgcgatgga
     3061 aaaggaatcg tacaggatct ctctttcttt gtcggtgagg gtggtaatcg aagtaatgat
     3121 ttgatgggcg tacttctgtg cattctcagt aagctgatcg gtgtctatct cca
//
LOCUS       DAOTIL010000244         3170 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminobacteriaceae bacterium isolate AMR_MDS_3026
            NODE_36131_length_3170_cov_3.288604, whole genome shotgun sequence.
ACCESSION   DAOTIL010000244 DAOTIL010000000
VERSION     DAOTIL010000244.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868050
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminobacteriaceae bacterium (wastewater metagenome)
  ORGANISM  Aminobacteriaceae bacterium
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae.
REFERENCE   1  (bases 1 to 3170)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3170)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:19:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,224
            CDSs (total)                      :: 3,198
            Genes (coding)                    :: 3,184
            CDSs (with protein)               :: 3,184
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3170
                     /organism="Aminobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_36131_length_3170_cov_3.288604"
                     /isolate="AMR_MDS_3026"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:3034609"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..376
                     /locus_tag="P5201_06145"
     CDS             <1..376
                     /locus_tag="P5201_06145"
                     /inference="COORDINATES: protein motif:HMM:NF012245.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HRV98149.1"
                     /translation="EQSKQSAKEVQRILRDIQKATSAAVMAIEQGSKAVEQGAKDAAP
                     SRESIQGITKKFVESAQSAAQIAAANNELLAGIDQVTQAMESIKAAGEQNVAGMKDLE
                     AASGGLKEMGQKLSLLIERYTV"
     gene            402..2729
                     /locus_tag="P5201_06150"
     CDS             402..2729
                     /locus_tag="P5201_06150"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hybrid sensor histidine kinase/response
                     regulator"
                     /protein_id="HRV98150.1"
                     /translation="MNQAEREFLEELRRDFVPEAEEYLQTIVSGLLALEKSPASSSGV
                     IEEVFRAAHSLKGAAQAVQMPGVSSICQVVESVFSGMKKGVLHLSEGDFDILQRAADT
                     LSAMIGAPEKEDASGITPLIHEIESLLDPAGGAAPPKTPLLQGSRRPNAEEDEPEGKD
                     EALSLPDRALPSHAVSPAVTEASPQPKEERSRHTGQETVRIAASKLDSLLLKAEELIS
                     VNLALEVRIENIREILSLSAEFKSEWEKTLDALRREEQAIEKLPQKLQGFLQSGRDSF
                     RKILETLRDMEKALRSDQRAASGLVRDLLEGARTVLMLPFSSLLQSFPKIVRDLAREL
                     GKEANVNIRGGEVEVDKRILEGLKDPLVHLVRNCVDHGLEKAEDRRAAGKPPEGTITL
                     TVSQTDGGRVEILVADDGKGINAEKLREIAVKNGLISREEAALLDDAAAMSLIFRSGV
                     STSPLITDISGRGLGMAIVQEGVEALGGSIVLESKPGKGSSFRLSLPLTLATFRGVLV
                     EEWGRVFVIPSSGVERVARVEKSKIRLIEQRESVSFNGTPLALARLGHILELPSPPER
                     LKIRETLPLVVIGAGGVSVAFGVDKVLNEQDILLKPLGKQLERVRNVSGATVLGSGMV
                     VPVLNTADLIRSVQRSGGGRGFLSDPEEKKTRRNVLIAEDSITSRTLLRNILSAAGFV
                     VQTAVDGQEAWDYLAKGGFHIVVSDVEMPRMNGFELTAKIRSDSATADIPVVLVTSLE
                     SREDRERGAEAGADAYIVKSGFDQGNLLDVLRRLL"
     gene            2756..>3170
                     /locus_tag="P5201_06155"
     CDS             2756..>3170
                     /locus_tag="P5201_06155"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HRV98151.1"
                     /translation="MADVLIVDDSALLRRILKQMLSKDPGLNVIGCVESGEAALQFLE
                     NTRVDVVTMDIHMPGMGGFDATRRIMESPHPVPVVIISSCWDPNEVEKTFEAMDAGAV
                     AILPKPADISSGDDEYSAELLKTVNEAALSKVGRLR"
BASE COUNT          712 a          787 c         1018 g          653 t
ORIGIN      
        1 agagcagtcg aagcagtccg cgaaagaggt gcagcgcatc ctccgcgata tccagaaggc
       61 cacgagcgcc gcggtgatgg ccatcgagca gggatcgaaa gccgtggagc agggagcgaa
      121 ggatgcggcg ccttccaggg agtcgattca agggatcacg aaaaaattcg tcgagtccgc
      181 gcagtctgct gcgcagatcg ccgcggcgaa caacgagctg ctggctggta tcgaccaggt
      241 gacgcaggcg atggagagca tcaaggccgc gggggagcaa aacgtggcag gaatgaagga
      301 cctggaagcg gcttcgggcg gtctgaagga gatgggacaa aaactctcgc ttctgatcga
      361 acggtatacg gtgtgaggcc ccgaggtacg aaggagcgct catgaaccag gcggagaggg
      421 aatttcttga ggaactgcgc agagattttg ttccggaggc ggaggagtat ctccagacca
      481 tcgtctccgg tctgcttgct cttgaaaaga gtcccgcaag ctcttccggg gtcatcgagg
      541 aggtcttccg ggctgcccat agcctcaaag gggcggcgca ggcggtacag atgccggggg
      601 tctcttcgat atgccaagtt gtggagagcg tcttttcagg catgaaaaaa ggggtccttc
      661 atctctccga aggagatttc gacatcctcc agcgtgctgc cgatactctg tccgccatga
      721 taggcgctcc ggagaaggag gacgcgagcg gcataactcc gctgattcat gagattgaaa
      781 gcctgttgga tccggcgggc ggcgccgctc cgccgaaaac gcctttgttg caagggagcc
      841 ggagaccgaa cgcggaggaa gatgagccgg agggaaaaga tgaagcgctc tcgcttccgg
      901 atcgcgctct tccgtctcat gcagtctctc cggctgtaac ggaagcctcc ccgcagccga
      961 aagaagagcg ctcacgacat accgggcagg agaccgtgcg tatcgccgcc tcgaaactgg
     1021 attcgttgct gctgaaggcc gaagaactga tttcggtgaa tcttgctctg gaagtccgca
     1081 tagagaatat acgggaaata ctgtcgcttt ccgcagagtt caaaagcgag tgggaaaaga
     1141 ctcttgacgc gctccggagg gaagagcagg ccattgaaaa gcttccccag aaattgcagg
     1201 gttttttaca gtcgggaaga gactcttttc gaaaaattct cgaaacgctg cgggacatgg
     1261 aaaaagcgct gcgttccgat cagcgtgccg cgagcgggct tgtccgcgat ctcctcgagg
     1321 gagccaggac tgtgctgatg cttccgtttt cgtcgctctt gcagagcttc ccaaaaattg
     1381 tccgcgatct tgcgcgcgag cttggtaaag aagcgaacgt gaacatacgg ggcggggaag
     1441 tcgaagttga caaacggatt cttgaaggat tgaaggatcc tctggttcat ctggtgcgca
     1501 actgcgtcga ccacggactg gaaaaggcgg aagatcgccg cgccgcagga aagcctcccg
     1561 aggggacgat cacgcttacg gtctctcaga ctgacggggg cagggtggaa attcttgttg
     1621 cggacgacgg aaaagggatc aatgcagaaa aacttcgcga aatcgccgtc aaaaacggcc
     1681 tgatctcaag ggaggaggcc gcgctcctcg acgatgccgc tgcaatgtcg ctcatcttca
     1741 ggtcaggcgt ttccacgagt ccgcttatta cggatatttc agggcgaggg ttaggaatgg
     1801 cgattgttca agaaggcgtc gaagcccttg gaggtagcat cgtccttgaa tcaaagcccg
     1861 ggaaggggtc ttcttttcgc ctttccctcc ccttaacgct tgcaacgttc cgcggagtgc
     1921 tggtggagga gtgggggcgt gtcttcgtta ttccatcctc tggtgtggag cgcgtggcca
     1981 gggtggagaa aagcaaaatc aggcttatag aacaaaggga gagcgtgtcg tttaacggga
     2041 cgccgctggc gctggcgaga ctgggacata tacttgagct gccgtctccc cctgaacggc
     2101 ttaagattcg ggaaacgtta cctctcgttg tgattggagc cggaggggtc tccgtggcgt
     2161 ttggcgtcga caaagtgctc aatgaacagg atattctgct caaaccgctt gggaaacagc
     2221 tggagcgggt gcggaacgtc tcgggcgcca ccgtgctcgg atcgggaatg gtcgttcccg
     2281 tgctcaacac ggccgatctt atccgttctg ttcagaggag cggcggggga cgaggttttc
     2341 tttccgaccc ggaagagaaa aaaacccgca gaaacgttct catcgccgag gattccatta
     2401 cttcgcgtac tctcctgcgg aatattctct cagccgcagg ttttgttgtg caaacagccg
     2461 tggatggaca ggaggcgtgg gattaccttg cgaagggcgg gtttcatatc gtggtctccg
     2521 acgtcgaaat gccccggatg aacgggttcg agctgacggc gaaaatccgc tccgactctg
     2581 cgacggctga tatcccagtg gtgctggtga catctcttga atccagagag gacagggaac
     2641 ggggcgcgga ggccggagcc gacgcatata tcgtgaaaag cggtttcgac cagggaaacc
     2701 ttcttgatgt attgcgtcgt cttctgtaga gtgtaaaaga gagggggcgt tcggcatggc
     2761 ggatgttctc attgttgatg attccgcgtt gctgcgcagg atcttgaagc agatgctgag
     2821 taaggatccg gggctgaacg ttatcgggtg tgtcgagagc ggcgaagcgg cgttgcagtt
     2881 cctcgaaaac accagggtcg acgttgtgac tatggatatt cacatgccgg gcatgggcgg
     2941 tttcgacgca acacgccgga tcatggaatc tccgcatcct gtgcctgtgg tgattatcag
     3001 ttcatgctgg gatccgaacg aagtcgaaaa gacgttcgag gctatggatg caggcgccgt
     3061 ggctattctc cccaagcccg cggatatctc ctcaggcgat gacgaatact ctgcggagct
     3121 tctgaaaact gtaaacgagg ctgctttgtc aaaagtagga cgtctgcggc
//
LOCUS       DAOTIL010000245         3168 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminobacteriaceae bacterium isolate AMR_MDS_3026
            NODE_36162_length_3168_cov_3.303887, whole genome shotgun sequence.
ACCESSION   DAOTIL010000245 DAOTIL010000000
VERSION     DAOTIL010000245.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868050
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminobacteriaceae bacterium (wastewater metagenome)
  ORGANISM  Aminobacteriaceae bacterium
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae.
REFERENCE   1  (bases 1 to 3168)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3168)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:19:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,224
            CDSs (total)                      :: 3,198
            Genes (coding)                    :: 3,184
            CDSs (with protein)               :: 3,184
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3168
                     /organism="Aminobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_36162_length_3168_cov_3.303887"
                     /isolate="AMR_MDS_3026"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:3034609"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1801
                     /locus_tag="P5201_06160"
     CDS             <1..1801
                     /locus_tag="P5201_06160"
                     /inference="COORDINATES: protein motif:HMM:NF024487.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="AAA domain-containing protein"
                     /protein_id="HRV98152.1"
                     /translation="TSGLFEKIERPEWLEEAEALFLEKASERYGPLTNVASASERLHA
                     ELWECAESVGSLLDALLRTAPSSPDAKIGEETRRRLGELEESLESAAKELENGRRLLA
                     RAEETRILWEKHLASESFLVALLSFLPPVRKRKEAADRAFLLERSIACESADREKIRE
                     ALARQREEAAKGASEAEARLNERTRALSSLEESWSKAASLASLARINLDAEDVSLNEA
                     TERLDTSVRAKAFRVASHYWEAEYLRELRGNIESKYRDSAAPDKRARMYRRFAKLVPC
                     QIATFFMLPNYYCGYRGKPIHMYGEIDLLIVDEAGQVAPEIGVPSFSLAKRALVVGDI
                     FQIEPVWSVAVGTDSANAVESGVVRSEEEYEELEKRGFFAASGNLMRMAQLACRHTQY
                     PELSPGMMLVEHRRCVPEIIAYCNDLVYKGKLEPRRRESEEERRSRPSFLPAMGEIPT
                     VSDDERASGSRRNPEEAARIAEWTKEHLKELETRYEKPIGEILGIVTPFAAQKKAVRK
                     ALADALGDDRGVVTGTVHALQGAERDVVLFSPVYGRGHSGNTFFDAGPNMMNVAVSRA
                     RDSFIVVGNPDLFVEGSGKPSGILARHLRKPSL"
     gene            complement(2086..2817)
                     /locus_tag="P5201_06165"
     CDS             complement(2086..2817)
                     /locus_tag="P5201_06165"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRV98153.1"
                     /translation="MVRFLKGAVLSLVFLFSIGSAALCSDILESVVSAAADAHREEMK
                     DAQGAVPTTAFTALGQTFGAAFGFEFDEERMSRLWQACRTPSLARSDDAVSAVHEMAR
                     VCGVSGYEVAVLKPENTLFLSERALRDYHADGAVMVFFEDMTAKTRRETERNVKFFDN
                     LFTLLPGSKSGRVLRDTAKREILANSARTVQSGARLLSFEKGAATLSSGSERIELSLT
                     ELENRSAYIVGMRRSREVEAAPQVK"
BASE COUNT          649 a          956 c         1004 g          559 t
ORIGIN      
        1 gacgagcggc ctcttcgaga agatagagcg tcccgagtgg ctcgaagagg ccgaagcgct
       61 ctttctggag aaggcgtcgg agaggtacgg cccccttacg aacgtcgcct cggcgtcgga
      121 acggctgcac gcggagcttt gggagtgcgc ggagagcgtc ggttcgctcc tcgacgccct
      181 ccttcgaacg gcgccttcct ccccggatgc gaagatcggc gaggagacgc gccgccgcct
      241 cggggaactc gaagagagtc tggaaagcgc cgcgaaagaa ctcgagaacg gaagacgtct
      301 cctcgcccgc gcagaggaga cccgtattct ctgggagaag cacctcgcct ccgagtcctt
      361 tctcgtggcg ctcctttcat ttctgccacc cgtgagaaaa agaaaagagg cggcggaccg
      421 cgcctttctg ctcgagcgga gcatcgcctg cgaatccgcc gacagagaga aaatccggga
      481 ggcgcttgcc cgccaaagag aggaggccgc gaagggggcg tcggaggcgg aagcgagact
      541 gaacgagcgc acgagggcgc tctcgtcgct ggaggagagc tggtcgaaag ctgcgtctct
      601 cgcctcactc gcccggataa acctcgacgc cgaagacgtc tcgctgaacg aagcgaccga
      661 gcgtctggac acatccgtcc gggcgaaggc cttccgcgta gcgagccact actgggaggc
      721 cgagtacctg agggagcttc gaggcaacat cgaatcgaag tatcgggaca gcgccgcgcc
      781 cgacaagagg gcgcgcatgt accgccgctt tgctaagctg gttccgtgcc agatagcgac
      841 cttcttcatg ctcccaaact actactgcgg ctacaggggg aagccgatcc acatgtacgg
      901 cgagatcgac ctcctcatcg tcgacgaggc cggacaggtc gccccggaga taggtgttcc
      961 ctccttttcg ctcgcaaaaa gagcgctcgt cgtcggcgac attttccaga tagaacccgt
     1021 ctggtcggtg gccgtcggga cggactcggc caacgcggtc gaaagcggcg tcgtccgttc
     1081 ggaggaggag tacgaggagc ttgagaagag gggctttttc gccgcctccg gcaatctgat
     1141 gcgcatggcg cagctcgcct gccgccatac ccagtaccct gaactctccc ccggcatgat
     1201 gctcgtcgag caccgtcggt gcgtccctga gatcatcgcc tactgcaacg accttgtcta
     1261 caagggaaag ctcgagccgc gcagaagaga gagcgaggag gagcgacgaa gccgcccctc
     1321 cttcctgccg gcgatggggg agatcccgac ggtctccgat gatgagcgcg cctcgggaag
     1381 ccgcaggaac ccggaggaag ccgccaggat agccgagtgg acgaaggagc atctgaagga
     1441 gctcgaaacg aggtacgaga agccgatagg ggagatactg gggattgtca cgcccttcgc
     1501 cgcccagaag aaagccgtcc ggaaggcgct cgccgacgct ctcggcgacg accggggcgt
     1561 cgtgaccggc acggtccacg ccctgcaggg agccgagagg gacgtggtgc tcttctcccc
     1621 ggtctacggc cgcggacact ccgggaacac cttcttcgac gcggggccga acatgatgaa
     1681 cgtcgccgta tcgcgcgcgc gggactcctt catcgtcgtc gggaatccgg atctcttcgt
     1741 ggaggggagc ggtaagccat ccgggattct ggcgcggcat ctgcggaagc catccctttg
     1801 acgcgcgctt cgcttcgaga agggaagaga tcatgagtcc tccgagaaag gatgcgtcga
     1861 aggaactctt ccggaagaat tcagcgaagg gggagggtgg tgcttcaccg accttctctt
     1921 cggcggagaa caagcagccc cagccgagcg tctccggaaa gaacggaagg ctgtctccgc
     1981 gcgcgtgcgg ttcgggtaga aggacggaaa ttgaatgacg tattgattcc ggtgttgccc
     2041 gagcacatag aagattcgct gcgcgggtag cggcagtacg gtgttctatt tcacttgcgg
     2101 cgcggcctcg acctctctgc tcctccgcat ccccacgatg tacgcgctcc tgttttcaag
     2161 ctccgtcaag gagagttcga tcctctccga gccggacgag agcgtcgcgg cccccttttc
     2221 gaaagagagc aacctcgcgc cgctctgaac cgttctcgcc gagttcgcaa ggatttctct
     2281 cttcgcggtg tcacgcagaa cgcgtccgct cttcgaacct ggtaaaagag tgaagagatt
     2341 atcgaagaac ttcacatttc gctccgtttc gcgcctggtc ttcgccgtca tgtcttcgaa
     2401 gaataccatg acggcgccgt cggcgtggta gtcccgcaga gccctctcgg agaggaagag
     2461 cgtgttctcg ggcttgagaa cggcgacttc gtacccggag actccgcaga cccgagccat
     2521 ttcgtggact gcggaaacgg cgtcgtccga cctcgcgaga gaaggtgtcc ggcaggcttg
     2581 ccagagcctc gacattctct cctcgtcgaa ctcgaaaccg aacgccgcgc cgaacgtctg
     2641 tccgagcgcg gtgaaggcgg tcgtcggaac ggcgccctgc gcgtctttca tctcttccct
     2701 gtgagcgtcc gccgccgccg agaccacgga ttcaaggata tcggagcaga gggcggcgga
     2761 gccgatcgag aacaggaaca ccaacgaaag aaccgcaccc tttaggaatc tcaccatact
     2821 cgcctcctct tcgtttcgag agagcgccga gtccgctgcg ctcgtctctc cctgcgaaaa
     2881 aacgcagttt ccgacgggcc tctcctcgcg tcccgagggt tcgcatcgcg cgccggcgcc
     2941 gctctttccg catccattat agactcgctt gaaagtctcc tcaagccgaa tcgatttcag
     3001 gtgtgacgag acagtgataa tgtcatcctc agacgggaca gggaaaatgt catctgtcat
     3061 tctaagtgaa tgatgaggag cggtgttttc ttgagcctga aagaaacgac aaaagcggtg
     3121 attctggaca gagtggtgaa aggagagatg acggcggcgg aaggagcg
//
LOCUS       DAOTIL010000246         3167 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminobacteriaceae bacterium isolate AMR_MDS_3026
            NODE_36179_length_3167_cov_4.011568, whole genome shotgun sequence.
ACCESSION   DAOTIL010000246 DAOTIL010000000
VERSION     DAOTIL010000246.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868050
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminobacteriaceae bacterium (wastewater metagenome)
  ORGANISM  Aminobacteriaceae bacterium
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae.
REFERENCE   1  (bases 1 to 3167)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3167)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:19:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,224
            CDSs (total)                      :: 3,198
            Genes (coding)                    :: 3,184
            CDSs (with protein)               :: 3,184
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3167
                     /organism="Aminobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_36179_length_3167_cov_4.011568"
                     /isolate="AMR_MDS_3026"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:3034609"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1101)
                     /locus_tag="P5201_06170"
     CDS             complement(<1..1101)
                     /locus_tag="P5201_06170"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HRV98154.1"
                     /translation="MEKQLLTLEKLQNRILSLEEERLALSNALDIALKINDIQPDAAA
                     ETTVESILSVCCERTGRLIGLKEGGFYLFREEDAVFYQAFASSPAAKERIEHELQLLI
                     DDGTIAWVIQTERPVTVRLSSGEDAFLHALAAPGRTMGAFIGILREPKEEILDISYAL
                     LSIYFSQVAGVLQNRELLRMARIENRHLTSTVDVLRETEEKLVSLNRTLEEMVEERTR
                     ELEKTNRLLKEEIAERKRKEEALLESEELSASLLNHSPHPILVVNPDTSVKYVNFALE
                     QITGYSLVEVINRKAPYPWWGEEKLSTVSEYYRMVQSGETAKKEYCFRKKNGSPLWVE
                     VSMTSVSGSAGAKYAVANWVDITDRKVAERARE"
     gene            complement(1076..2344)
                     /locus_tag="P5201_06175"
     CDS             complement(1076..2344)
                     /locus_tag="P5201_06175"
                     /inference="COORDINATES: protein motif:HMM:NF020252.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HDOD domain-containing protein"
                     /protein_id="HRV98155.1"
                     /translation="MGTLSITQLKSGMILSADLVAPNGRFILPKGACLTSRNIMSLKI
                     WGVSEVAVSDRSDHPCEEDEFLYSSSVMEEARTLVLRIFGGIPPGNRAMEEVYRVAIR
                     EAARSLTENEARPPVFFRPEFTPPSGAPLPKLAMEELVGAEVRFVSFPDVYFRISEAL
                     QSPKSSITHVAELISKDTALSATLLKLANSAIYGVPAKVDSIQRAAALIGGKALSILA
                     LGVSAVKSFNSIPSEWVNMESFWRHSVAVAVLGQIISSRKNSRIIEKVFVGGILHDIG
                     RLVLLRHVPSVMARAIAAAAEQKVPLVEMERELFGFDHGELGGSILERWGFPRELSDL
                     VRLHHAPVDETHSFPAAALSVADTLAAALRQGSSGSPHPPAISDEVWNMTDLSPGALD
                     SVVRQARRQISEIYSLFKGGDDDGKTVAHS"
     gene            2619..>3167
                     /locus_tag="P5201_06180"
     CDS             2619..>3167
                     /locus_tag="P5201_06180"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRV98156.1"
                     /translation="MPSLFSVLSRASAHHPLNRRLFLLAVLLFFFQSAACANELPWVE
                     HSVLRGETADSIAAKYAVSSKTVERANELAARTEPLKTGEKLLVPKKETDLLSTLAEV
                     RARRRGESPLSSITREQSKKVSLPAPRKSPLPRPAEPFIRPLAGRITSPYGMRGGRLH
                     DGVDIPAPKGTPIVASRPGKVVF"
BASE COUNT          593 a          997 c          931 g          646 t
ORIGIN      
        1 ctccctggcg cgctccgcca ccttcctgtc cgtgatgtcc acccagttgg cgaccgcgta
       61 cttggcgcct gcgcttcccg atacggaggt catgctcacc tccacccaga gcggggagcc
      121 gttcttcttg cggaagcagt actccttctt cgcggtctcc ccggactgca ccatccggta
      181 gtattcggaa acggtcgaaa gcttttcctc tccccaccac gggtaggggg ccttccggtt
      241 gatcacctcg accagcgagt agcccgtgat ctgttcgagg gcgaagttga cgtatttgac
      301 cgacgtatcg gggttcacca cgaggatggg gtgcggcgag tggttcagaa ggctcgcgga
      361 gagctcctcg ctctcgagga gggcctcttc cttccgcttc ctctcggcga tctcctcctt
      421 caggagtctg ttcgtcttct ccagctccct ggtgcgctcc tcgaccatct cctcaagcgt
      481 ccggttgagg gagaccagct tctcctccgt ctcccgcagc acgtccaccg tcgaggtaag
      541 atgccggttc tcaattctcg ccatgcggag cagctccctg ttctggagca ctccggccac
      601 ctggctgaag tagatggaga gcagggcgta gctgatgtcg aggatctcct ccttcggctc
      661 cctcaggatg ccgatgaacg cccccatcgt cctccccggc gcagcgaggg cgtggagaaa
      721 ggcgtcctcc ccggatgaga gacgcaccgt cacggggcgc tccgtctgga tgacccacgc
      781 gatggttccg tcgtcgatca gaagctgaag ctcatgctcg atacgctcct tcgcggcggg
      841 cgagctggca aacgcctgat agaagaccgc gtcctcctcc cggaagaggt agaagccgcc
      901 ctccttcagc ccgatgagcc tgcccgtccg ctcgcagcat acggagagga tggactccac
      961 cgtggtctcg gcggctgcgt ccggctgaat atcgttgatc ttgagcgcta tgtcgagcgc
     1021 gttggacagg gcaagccgtt cctcctccag cgagaggatt ctgttctgaa gtttttcaag
     1081 agtgagcaac tgtttttcca tcgtcatcgc cgcctttgaa aaggctgtat atctccgaaa
     1141 tctgacgtcg cgcctgccgc accaccgaat ccagcgctcc gggggagagg tccgtcatgt
     1201 tccagacctc gtccgaaatc gccggagggt gcggcgagcc gctgcttccc tgccgcagcg
     1261 cggccgcgag cgtgtccgcg acggagagcg ccgccgcggg aaaggagtgc gtctcgtcga
     1321 cgggagcgtg atgcagccgg acgagatccg agagttcgcg ggggaacccc catcgttcga
     1381 ggatactccc tccgagctcg ccgtgatcga atccgaagag ttcgcgctcc atctccacga
     1441 gggggacttt ctgttccgcc gcggccgcta tcgccctcgc catgacggac gggacgtgcc
     1501 gcagcaggac gagcctgccg atgtcgtgga ggatgccccc gacgaaaact ttttctatga
     1561 tccgcgaatt cttcctcgat gagatgatct gtcccaggac cgccacggcg accgagtgtc
     1621 tccagaagga ttccatgttc acccattcgg atggaatgct gttgaaggat ttcaccgccg
     1681 atactccgag ggcgaggatg gaaagcgcct tgcccccgat gagggcggcg gcgcgctgaa
     1741 tggagtccac cttcgccgga acgccgtaga tggcgctgtt ggcgagtttc agaagcgtgg
     1801 cggagagggc cgtatccttg ctgatgagtt cggccacgtg cgtgatggag cttttggggg
     1861 attgaagcgc ctcggatatg cggaagtaga cgtcggggaa ggagacgaaa cgaacttcgg
     1921 ccccgacaag ctcttccatc gcgagcttcg gcagcggcgc tccggaaggc ggggtaaatt
     1981 ccggcctgaa aaaaacgggg ggacgcgctt cattctccgt caggcttcgc gcggcctcgc
     2041 ggatggcgac ccgatagact tcttccatcg cccggtttcc gggggggatg ccgccgaata
     2101 tgcggaggac gagcgtgcgc gcttcctcca tgacggagga ggagtacaga aattcgtcct
     2161 cctcgcaggg atgatccgaa cggtccgaca cggcaacctc ggatactccc cagatcttca
     2221 gactcatgat gttcctggac gtcaggcatg cgcccttcgg cagaatgaac cgcccgttcg
     2281 gggcgaccaa gtccgccgaa agaatcattc cggatttcag ttgagtgatg gaaagtgttc
     2341 ccatgcaacc ccccggaatt cccctcacgg ttcaaagggg agaataaaga aaatttttca
     2401 ccgctctcct ttttaaacca cagagtgcag acgctgtcaa gggcttggac acgaagctgt
     2461 tacgggaggg cgaaaatgtc gcccctcaat gttcaaaaat ggaacaggaa gacgcccttg
     2521 tagtaaattc gttctcagta tttttaccgt ccgcaacctt ctgttttcat gtatcatacc
     2581 atagagcgtc ataaaataaa gttcaggagg aactccgcat gccctctctc ttcagcgtcc
     2641 tgtccagggc ttctgcgcat catccgttga accgtcgtct ttttcttctc gctgttcttc
     2701 ttttcttttt ccagtccgcc gcgtgcgcga acgaacttcc gtgggtcgaa cactcggtcc
     2761 tccgcggaga gaccgccgac tccatcgcgg cgaagtacgc cgtttcctca aagaccgtcg
     2821 aaagggccaa tgaacttgcc gcccggacgg aacctctgaa gacgggggaa aagctcctcg
     2881 ttccgaagaa agaaaccgat ctcctctcca ccctcgccga ggttcgcgcc cggcggcggg
     2941 gcgaatcacc gctcagttcg atcacgaggg agcagtcgaa aaaggtttct cttcccgcgc
     3001 cgaggaagag ccccctgccg cgaccggccg agcccttcat ccgcccgctc gccggaagaa
     3061 tcacctctcc ctacggaatg cgcggcggcc gcctgcacga cggcgtggat atcccggctc
     3121 ccaagggaac gccgatcgtc gcgtcgcgcc ccggcaaggt cgtcttc
//
LOCUS       DAOTIL010000247         3166 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminobacteriaceae bacterium isolate AMR_MDS_3026
            NODE_36194_length_3166_cov_4.203472, whole genome shotgun sequence.
ACCESSION   DAOTIL010000247 DAOTIL010000000
VERSION     DAOTIL010000247.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868050
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminobacteriaceae bacterium (wastewater metagenome)
  ORGANISM  Aminobacteriaceae bacterium
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae.
REFERENCE   1  (bases 1 to 3166)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3166)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:19:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,224
            CDSs (total)                      :: 3,198
            Genes (coding)                    :: 3,184
            CDSs (with protein)               :: 3,184
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3166
                     /organism="Aminobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_36194_length_3166_cov_4.203472"
                     /isolate="AMR_MDS_3026"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:3034609"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..289)
                     /locus_tag="P5201_06185"
     CDS             complement(<1..289)
                     /locus_tag="P5201_06185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012869035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HRV98157.1"
                     /translation="MKLSESRSLRKYALLLILASFLLYFGGLGGYGLLEPDEGRYSEI
                     PREMLETGDFVTPRLNYVKYFEKPVLHYWLTAASLAVFGENEFAGRFWPALL"
     gene            450..821
                     /locus_tag="P5201_06190"
     CDS             450..821
                     /locus_tag="P5201_06190"
                     /inference="COORDINATES: protein motif:HMM:NF013089.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SMR family transporter"
                     /protein_id="HRV98158.1"
                     /translation="MDRLSLFLLAGSAFFNALANTMMKFAFGHQKSLLDGGVVSAVLS
                     IVLNPWAVAGVGCFGVSFVFLSAALTRTDLSIAYPFMSGLVFLLILAVSTLFFSEQVN
                     VWRLAGMGSILAGIWMIAVKG"
     gene            complement(857..1594)
                     /locus_tag="P5201_06195"
     CDS             complement(857..1594)
                     /locus_tag="P5201_06195"
                     /inference="COORDINATES: protein motif:HMM:NF013618.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase SLT domain-containing protein"
                     /protein_id="HRV98159.1"
                     /translation="MSLLIRRSITALFLFSALLFLLIGIEGEKIRFMIADMEIFRPPN
                     LGSTEGFWSVNPEMIELFQNAGRTIPSSFSDIARTFTSAGHVAATLFEATAENIRNLA
                     VFITEQNANIPMESALIQAAAFWKYSIKYEVPLDLIVAVAHTESHFNPEARSGAGAAG
                     VMQVMWKVHSGLLQANGILSEEELHNPDLGIAAGSLLLSRYLRTHEDLQTALGRYFGG
                     PASVYWKKVSRNLEKLRKANIVAPSDI"
     gene            complement(1724..>3166)
                     /locus_tag="P5201_06200"
     CDS             complement(1724..>3166)
                     /locus_tag="P5201_06200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013047529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0182 family protein"
                     /protein_id="HRV98160.1"
                     /translation="VAGSGLPRLWVRDLPPRSEVDLPITHPQIYYGEKPSSYIFVRTT
                     VQEFDYPMGDSNVRTVYEGKGGVPMGSFFRRILFALKFADMKILLSDVFTEESRVKFH
                     QNIQERLMRVAPFLYFDRDPYLIVSEGRLVWMQDAYTAADSYPYAQPVSLGRGQNINY
                     IRNSVKATVDAYDGTMRFYAADPDDPVLKAWSGIFPEIFKPYSEMPKGLKAHLRYPQD
                     LFNIQSEIYRTYHMTDANTFYNKEDVWDRPGSADSSGSAADAYYVNMRLAGEKHAEFA
                     LISPFMPVSRNNMIAWMAGRSDGDNYGQLLVYQFPKQKLIYGPAQVEALTNQNSEISA
                     QLSLWSQRGSSVVRGNMTVVPVGNGLLYVQPLYLRAENSELPELRRVITSTGGRVVWG
                     ETLEESLVRLLSPTASELETSSLVPAETTERTEFSGSEALAELAAEAMTHWDASQEAL
                     RKGDWEGYGRGMSGLENVLREMMDRKDREQ"
BASE COUNT          606 a          896 c          940 g          724 t
ORIGIN      
        1 cgaggagcgc gggccagaat cgacctgcga actcgttctc gccgaaaacg gcgagggacg
       61 ccgcggtcag ccagtagtgg agcacgggtt tttcgaagta cttgacgtag ttcagccggg
      121 gagtgacgaa atccccggtc tccagcatct cgcggggtat ctcggagtac ctcccctcgt
      181 ccggttccag aaggccgtag cctccgagac cgccaaaata gagaagaaac gaggccaaaa
      241 tgagaagaag cgcatacttt cgtaacgaac gcgactcgga gagtttcatc gaaccaccct
      301 tccaaaaaaa gatccaatcg catcattgta catgacgttc ccttatttgt ggtagtgcgc
      361 gggagagtat atacttcttc ggagtgaaac gtgaccggat accccgccgg cagccggagg
      421 gggaaatgtg aaagggtgga tacttctcta tggacaggct ttcgttgttt cttcttgcag
      481 gatctgcgtt cttcaacgcc ctggcgaaca cgatgatgaa gttcgccttc gggcatcaga
      541 aaagtcttct cgacggggga gttgtctcgg cagtgctctc catcgtgctc aacccttggg
      601 ccgtagcggg ggtcggctgt ttcggcgtct ccttcgtctt tctgagcgcc gcgctcacca
      661 ggacggatct ctccatagcc tacccgttca tgtcggggtt ggtctttctg ctcattctcg
      721 ccgtctccac gcttttcttc tccgaacagg tgaacgtctg gaggctcgcc ggaatgggct
      781 cgatactcgc cggaatctgg atgatcgccg tcaaagggta aatgcgaacg cgcggattcc
      841 cttccttcgt gctctctcag atgtcgctcg gcgcgacgat attcgccttg cggagttttt
      901 cgaggttacg ggacactttc ttccagtaga cggatgccgg accgccgaag tagcgtccga
      961 gggcggtttg caggtcctcg tgcgtccgca ggtagcgtga gaggagaagg ctgcccgccg
     1021 ctattcccaa gtcggggttg tggagctcct cctcggagag aatcccgttg gcttgcagca
     1081 ggccggagtg caccttccac atcacctgca tgacgcccgc tgcgccggcg ccgctacggg
     1141 cttccgggtt gaagtggctc tccgtgtgcg cgacggcgac gatcagatcg aggggaacct
     1201 cgtatttaat ggagtatttc caaaaggctg cggcctgtat cagtgcggat tccatgggaa
     1261 tgttcgcgtt ctgttccgtg ataaaaacgg caagattccg tatgttttcg gctgtcgctt
     1321 cgaagagcgt ggcggcgacg tgccctgccg aggtgaatgt cctggcgata tcggagaacg
     1381 acgatggaat tgtccgtccg gcattttgaa agagctcgat catctcgggg ttgactgacc
     1441 agaaaccttc agtactccca aggttgggcg gtctgaatat ctccatgtcc gcgatcatga
     1501 accggatttt ttccccttct attccaatga gaagaaagag caatgccgaa aaaagaaaaa
     1561 gtgcggtgat gcttctccgt atcaagagcg acatttcgtt cccctccctc ttattcaaag
     1621 aaatgcgacc ttccaaagga agggccgatg gaaggtcgca ttattatagc actctcaaga
     1681 gtctgaatgc gtgtattttc tttacaatac ggcgccagct gagctactgc tcccggtctt
     1741 tccggtccat catctcacgg agtacgtttt ctagtccgct catccccctg ccgtacccct
     1801 cccagtcgcc tttccgcaat gcttcctgag aggcgtccca gtgcgtcatc gcttcggctg
     1861 caagttcggc gagcgcttcc gatcctgaga actccgtccg ctccgttgtt tcagccggaa
     1921 caagggagga tgtttccagt tccgacgccg tgggagagag caggcgaacg agcgattcct
     1981 cgagcgtttc tccccagacc acccggcctc ctgtcgatgt gatcacgcgc cgtagctcgg
     2041 gaagttcgct gttctcggcc ctgaggtaga gcggctggac gtagagaagt ccgttcccca
     2101 caggtaccac cgtcatgttg cccctgacga cggacgagcc tcgttggctc cagagggaga
     2161 gctgtgcgga gatctccgaa ttctggttcg tgagcgcttc tacctgcgcg gggccgtaga
     2221 tgagcttctg cttcgggaac tggtagacaa gcaactggcc gtagttgtcc ccgtccgatc
     2281 ggcccgccat ccacgcgatc atgttgttcc tgctcaccgg catgaacgga gaaatgaggg
     2341 cgaactccgc atgtttctct cctgcgagcc gcatgttcac gtagtatgca tcagctgccg
     2401 acccgcttga atcggcgctt ccgggcctgt cccacacgtc ctctttgttg tagaaagtgt
     2461 tcgcgtccgt catgtggtac gtgcggtaga tctcgctctg gatgttgaag aggtcctgcg
     2521 ggtagcgcag gtgcgctttc agccccttcg gcatttccga gtacggtttg aatatctccg
     2581 ggaagatgcc gctccacgcc ttcagcaccg ggtcgtcggg gtctgcggcg tagaagcgca
     2641 tcgtgccgtc gtaggcgtcg actgtggcct tcacgctgtt gcgtatgtag ttgatgttct
     2701 gtccccgtcc gagcgagacc ggctgcgcgt aggggtagct gtccgcggca gtataggcgt
     2761 cctgcatcca gacgaggcgt ccttccgaga ctataaggta cggatccctg tcgaagtaga
     2821 ggaacggcgc gacgcgcatg aggcgttcct ggatattctg gtggaacttc acgcggctct
     2881 cctcggtgaa gacgtcggag agaaggatct tcatatcggc gaacttcagc gcgaagagaa
     2941 tgcggcggaa gaaggaaccc atcggcacgc cgcccttccc ctcgtacacc gtgcggacgt
     3001 tggagtcgcc catcgggtag tcgaactcct gcacggtcgt ccggacgaag atgtaggagc
     3061 tgggcttttc gccgtagtag atctgcgggt gcgtgatggg aagatccacc tcgcttctgg
     3121 gaggaaggtc gcgcacccag aggcggggca gtccgctccc ggcaac
//
LOCUS       DAOTIL010000248         3150 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminobacteriaceae bacterium isolate AMR_MDS_3026
            NODE_36450_length_3150_cov_3.690145, whole genome shotgun sequence.
ACCESSION   DAOTIL010000248 DAOTIL010000000
VERSION     DAOTIL010000248.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868050
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminobacteriaceae bacterium (wastewater metagenome)
  ORGANISM  Aminobacteriaceae bacterium
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae.
REFERENCE   1  (bases 1 to 3150)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3150)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:19:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,224
            CDSs (total)                      :: 3,198
            Genes (coding)                    :: 3,184
            CDSs (with protein)               :: 3,184
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3150
                     /organism="Aminobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_36450_length_3150_cov_3.690145"
                     /isolate="AMR_MDS_3026"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:3034609"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..2267
                     /gene="alaS"
                     /locus_tag="P5201_06205"
     CDS             <1..2267
                     /gene="alaS"
                     /locus_tag="P5201_06205"
                     /EC_number="6.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013048416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004813 - alanine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006419 - alanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="alanine--tRNA ligase"
                     /protein_id="HRV98161.1"
                     /translation="DEEAYDVWHNIVGLPDERIFRFDKDANFWFMGTQGPCGPCSEIM
                     YDQGPEFSCGRPECAVGCDCDRYLEIWNLVFTQFDLQKDGSLVPLPKKNIDTGMGLER
                     LTSVVQRVRTDFETDLFRPMIDRACSLGGIQYGYSKKTDLAVRVIADHIRSVVFMIAD
                     GILPSNDGRGYVLRRLLRRAARFGRLLGVDRPFLLDFVPDVIALMGDPYREIVDNRLM
                     VEQIIDVEEKRFGRTLQQGTELLDAEMANLKRSGSTMLSGAVAFELYDTFGFPLELTS
                     EIVEENGFTVDREGFDREMTAQRERARASSKQKRSEMTGDVYTELNEALGDTEFLGYS
                     ECASLSSVAALVRDGSQIDSVSAGEDVEVVLDRTPFYGEKGGQIGDTGMLQGDGFKVE
                     VTDTLIRAKGLRVHKAKVLEGSLKTGDSVRACVNTERLNSIRRNHTATHLLHEALVRV
                     LGGHVRQSGSLVGESGLRFDFTHFEPLTREQIAQVEREVNSEILANDSLNITETDMEH
                     ARAEGAKALFEEKYGDVVRVVRIAGFSAELCGGLHVNSTGEIGLFKILREEGIGSGTR
                     RITAATGLEALRVFQRTWRTLGEIGGALAVDETGVLPKIEQLQSEVKSLARTIEEMKI
                     NSLSSSLESSLRHASAGGVDVVFGRFDGVNADMLREMGDRVLNKRSVDTGVLVALASV
                     AEGNVTIIAMADEKALARGVSAGKVVKEGSAMLGGGGGGRPALAQGGGKKPEALEDFF
                     KALPGIVERQLLSC"
     gene            2261..2683
                     /gene="ruvX"
                     /locus_tag="P5201_06210"
     CDS             2261..2683
                     /gene="ruvX"
                     /locus_tag="P5201_06210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006300954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction resolvase RuvX"
                     /protein_id="HRV98162.1"
                     /translation="MLSRRTLALDIGTVRIGVAVSDPLGMFAQGIAVLAAASAWMEEL
                     DALVKTYDPETLLLGVPLRTNGARGPEAEKIEELGRVLGERYPSVKVVLHDERFSTVM
                     ATQALLEGDVSRKNRKGKVDKVAAALILQSWLDRSGGV"
     gene            2770..>3150
                     /locus_tag="P5201_06215"
     CDS             2770..>3150
                     /locus_tag="P5201_06215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006300955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair protein MutS"
                     /protein_id="HRV98163.1"
                     /translation="MTPMLTQYFEWKKQYPDCILFFRMGDFYEMFFDDAVKASAILDI
                     TLTARDAEKSIPMAGVPWHAVNGYLGRLVRAGCKVAICEQITEPSNKGIVERKVIRIV
                     TPGTYVPEEAGSGGRLAAVCRAGKE"
BASE COUNT          581 a          863 c         1140 g          566 t
ORIGIN      
        1 atgacgagga agcgtacgac gtctggcaca acatagtcgg acttcccgac gagcggattt
       61 tccgcttcga caaggacgcg aacttctggt tcatggggac gcaggggccg tgcggtccgt
      121 gttccgagat catgtacgac caggggccgg agttttcgtg cggcaggccg gagtgcgccg
      181 tggggtgcga ctgcgacagg tacctcgaaa tctggaatct ggtgtttacg cagttcgacc
      241 tacagaagga cggctctttg gttccgttgc cgaagaagaa tatcgacacc ggtatggggc
      301 ttgagcgtct gacttcggtt gtgcagcggg tgcgcaccga ttttgagacg gatctcttcc
      361 ggccgatgat cgaccgggcg tgcagcctgg gcggcattca gtacggctac agcaagaaga
      421 ccgaccttgc cgtgcgcgtc atcgcggacc acatccgttc agttgtcttc atgatcgcgg
      481 acggcatcct gccgtcgaac gacgggcggg ggtacgtgct ccggcggctg cttcgacgtg
      541 cggcgcgctt cggccgtctg ctcggcgtcg accgcccctt cctgctcgac ttcgtccccg
      601 acgtgatcgc gctgatgggc gatccctacc gtgagatagt ggacaaccgc ctgatggtgg
      661 agcagatcat cgacgtggag gaaaagcgct tcggccgcac gctgcagcag ggaacggagc
      721 ttctggacgc ggagatggcg aacctcaaac gttccgggag cacgatgctt tcgggcgccg
      781 tcgccttcga gctgtacgat acgttcggct tcccgctgga gctgacgtcc gagatcgtcg
      841 aggaaaacgg cttcaccgtg gaccgcgaag ggttcgaccg ggagatgacg gcccagcgtg
      901 agcgtgcgcg cgcgtcgagc aagcagaagc ggagcgagat gaccggcgac gtgtacaccg
      961 aactgaacga ggcgcttggg gatacggagt tccttgggta ttcggagtgc gcttcgctgt
     1021 cgtcggttgc ggcgcttgtc cgcgacggca gtcagatcga ctccgtctcc gcgggcgaag
     1081 atgtcgaggt ggtgctcgac cggacgccgt tctacggcga gaagggcgga cagatcggcg
     1141 ataccggaat gctgcagggc gacggattca aggtcgaggt gacggatacg ctgattcgcg
     1201 ccaaagggct gcgcgtccac aaggcgaagg tgctcgaagg ttcgctgaag acgggcgatt
     1261 ctgtgcgcgc ctgcgtgaac acggagcggc tgaactccat ccgcagaaat catacggcga
     1321 cccacttgct gcacgaggcg cttgtccgcg tgctcggcgg gcatgtgcgg cagtccggct
     1381 cgctcgtggg cgaatcgggc cttcgtttcg actttacgca ctttgagccg cttacgcgtg
     1441 aacagatagc gcaggttgag cgcgaggtga actcggagat tctggcaaac gattcgctga
     1501 acataacgga gaccgatatg gagcacgccc gcgccgaggg cgcgaaggcg ctcttcgagg
     1561 aaaagtacgg cgacgtggtc cgcgtggtgc gcatcgcggg cttttccgcg gagctgtgcg
     1621 gcggcctcca tgtgaactcg accggcgaga tcggcctgtt caagattctg cgcgaggagg
     1681 gcatcggctc cggaacccga aggattaccg cggcgaccgg actggaggcg ctccgcgtct
     1741 tccagcgtac ctggcggacg ctgggcgaga tcggaggcgc gctcgctgtt gatgaaacgg
     1801 gcgtgctgcc gaagatcgaa cagcttcagt cggaggtgaa gtcgctcgcc cggacgatcg
     1861 aagagatgaa aatcaactcg ctgagcagct cgcttgagag ttcgctccgg catgcttcgg
     1921 ccggcggcgt ggacgtggtc ttcggccgtt tcgacggtgt gaacgcggat atgctgcgcg
     1981 agatgggcga cagggtgctc aacaagcgtt ccgttgatac gggcgtcctg gtcgctcttg
     2041 cctccgtagc ggaggggaac gtgaccatta ttgccatggc cgacgagaag gcgcttgcca
     2101 ggggcgtgtc cgccggaaag gttgtcaagg aaggttcggc gatgctcggc ggcggcggcg
     2161 gcggaagacc ggcgctcgcg cagggcggcg gcaagaagcc ggaagctctg gaagacttct
     2221 ttaaagcgct tcccggcatt gtggagcggc agcttctttc atgctgagca gacggacgct
     2281 cgcccttgac atagggacgg tccgcatcgg cgtcgcagtc agcgatcccc tggggatgtt
     2341 cgcccagggg atcgccgttc tggctgctgc atccgcttgg atggaggagc tggacgcgct
     2401 tgtgaagacg tacgatcccg agacgctgct gctgggtgtg ccgctgcgca cgaacggcgc
     2461 gcgcgggccg gaggcggaga agatagagga gttggggcgt gtgctgggag aacggtatcc
     2521 gtcggtgaag gttgttttgc acgacgaacg gttctccacc gtcatggcga cgcaggcgct
     2581 tcttgaaggg gacgtttccc ggaagaaccg gaagggaaag gtggacaagg tggcggcggc
     2641 gctgattctg cagagctggc tggacaggag cggcggggta tgagcacagg ggctccggga
     2701 gagaaacacc tgccggagcg cggggaggcg gccgccgagg ccattcccgc aggactgtcc
     2761 ggggtgaaga tgacgccgat gctcacgcag tacttcgagt ggaagaaaca gtatccggac
     2821 tgcatcctct ttttccggat gggggacttc tacgagatgt tcttcgacga cgcggtgaag
     2881 gcgtcggcaa tccttgacat cacactcaca gcgcgggacg cggagaagag tatcccgatg
     2941 gcgggcgtgc cgtggcacgc ggtcaacgga tacctgggac ggcttgtccg cgcagggtgc
     3001 aaggtggcca tctgcgaaca gatcaccgag ccgagcaaca agggcatcgt cgaacggaag
     3061 gtgatccgca tcgtcacgcc ggggacgtac gtcccggagg aggccggttc gggcggaagg
     3121 ctggctgccg tgtgccgggc ggggaaggaa
//
LOCUS       DAOTIL010000249         3135 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminobacteriaceae bacterium isolate AMR_MDS_3026
            NODE_36676_length_3135_cov_3.696104, whole genome shotgun sequence.
ACCESSION   DAOTIL010000249 DAOTIL010000000
VERSION     DAOTIL010000249.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868050
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminobacteriaceae bacterium (wastewater metagenome)
  ORGANISM  Aminobacteriaceae bacterium
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae.
REFERENCE   1  (bases 1 to 3135)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3135)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:19:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,224
            CDSs (total)                      :: 3,198
            Genes (coding)                    :: 3,184
            CDSs (with protein)               :: 3,184
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3135
                     /organism="Aminobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_36676_length_3135_cov_3.696104"
                     /isolate="AMR_MDS_3026"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:3034609"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(146..637)
                     /gene="nusB"
                     /locus_tag="P5201_06220"
     CDS             complement(146..637)
                     /gene="nusB"
                     /locus_tag="P5201_06220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006300948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antitermination factor NusB"
                     /protein_id="HRV98164.1"
                     /translation="MNRGFLPQKRHRAREMALQLLYALDLRRSQTPEQAFELFPGDED
                     PEILEYARVLTSGAWERRMDIDNLIREYVVGWRPERMVAVDRAALRMCIYESLFAKCT
                     PVAVAISEAVELAKTFGTEESGKFVNGVLGRIVRNLPPDAAAQDASAFDAGDETHADD
                     ADS"
     gene            complement(642..1124)
                     /locus_tag="P5201_06225"
     CDS             complement(642..1124)
                     /locus_tag="P5201_06225"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRV98165.1"
                     /translation="MYLFWKRTSLGSILVSQDGMCDFVNSLCEKPFKCAQVSLSSADN
                     ALFLVITMPKDYDSSAMVSLETKLRDTFEKTGLSVRVSWVELAKTSFVDNEALASLAK
                     KPVVWAIAVALFALLLKEGVATLLWSLFWGGAFYAGVLFFQSEMGAELVRKLRGMAGR
                     "
     gene            complement(1225..1776)
                     /locus_tag="P5201_06230"
     CDS             complement(1225..1776)
                     /locus_tag="P5201_06230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006583402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HRV98166.1"
                     /translation="MDEMNKKLESEVLEEESAYEGVLGETGESEAVPEGMVAGVVHIS
                     EEVLSELAKKTLSSIDGVQPASPGIASKLGIGRKASEGVRVVLEDNGLHPMASVDVYI
                     LVKFGLRIPDLAWDVQEMIKKNIEEYTGYKVKAVNVNVQGIYFKNKVPAEAVAEEAKG
                     EVKQPEDSVPELDKSVQPEVEQL"
     gene            complement(1848..2276)
                     /locus_tag="P5201_06235"
     CDS             complement(1848..2276)
                     /locus_tag="P5201_06235"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRV98167.1"
                     /translation="MSAREKIAYLKGLLDGLGPVENEGQGKIFRAVVDALDALSLEVA
                     EHEDLIQEQRELYEDLADDCALLDEDLDSLEKAFSDFSGEGDLDDEDDEDEDDEFDRN
                     YVSVTCPHCSYVFYYQPEEYEPDEALECPGCGKDFEQPKE"
     gene            complement(2297..2869)
                     /gene="efp"
                     /locus_tag="P5201_06240"
     CDS             complement(2297..2869)
                     /gene="efp"
                     /locus_tag="P5201_06240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012869789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P"
                     /protein_id="HRV98168.1"
                     /translation="MAQVVDTSDFYSGIKIRWQEGIWEVIDFQHHKMGRGGATVRTKM
                     KNLDTGTIIENSFRSGERFERIIFDEKPAQFSYKEGDSYIFMDLSTYDQLSIPADILG
                     NVVNFLMDDMEVQIEFFEGRIMGIEMPKSVELKVVDTPPSFKGDTVSGGGKPAKLETG
                     ITISVPIFVNVDDTIVVDTRSGQYLERAKK"
     gene            complement(2987..3133)
                     /locus_tag="P5201_06245"
     CDS             complement(2987..3133)
                     /locus_tag="P5201_06245"
                     /inference="COORDINATES: protein motif:HMM:NF012485.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRV98169.1"
                     /translation="MPILSNIPLLGELFKYRKDVRDKSEVAMIVIPYILTTPDGPVEQ
                     FTLK"
BASE COUNT          748 a          955 c          725 g          707 t
ORIGIN      
        1 cgaaatacgg ctttccttcc ccaggagggt gaaatagaca tgaccgctgg aatgcttctt
       61 cagttcgctc aactcgccgc gaacgccaac ggcctgcata acggggtcct tcagaaaaag
      121 cgaggcgata cggagggaaa tctcgtcaac tgtcagcgtc gtcggcatgc gtttcatcac
      181 cagcgtcaaa tgcggacgca tcctgcgcag ccgcatcagg aggaagattc cggacaatac
      241 gccccaaaac gccgttgacg aacttgccgg attcctcggt accgaaggtc ttcgccagtt
      301 caacggcctc cgaaattgcg accgcgacgg gagtgcattt tgcgaacagg ctttcataaa
      361 tgcacattct cagagccgcc ctgtccaccg cgaccatgcg ttcgggacgc catccaacaa
      421 cgtattcacg gatcagattg tctatatcca tacggcgctc ccatgcgccg gaagtcaaaa
      481 cgcgcgcgta ctccaaaatc tccggatcct cgtcgcctgg gaacagctcg aacgcctgtt
      541 ccggggtttg gcttctccgg agatcaagcg catacaataa ttgaagcgcc atctcacgtg
      601 cacgatgacg cttctgcggc aaaaaaccac gattcactcc attacctccc cgccatgccc
      661 ctcagctttc gcacaagttc agcgcccatc tccgactgga agaaaagaac tcccgcgtaa
      721 aatgcgccgc cccagaaaag actccataga agcgtggcca cgccctcctt gagcaagagg
      781 gcaaaaaggg cgacggcaat cgcccagacg acaggttttt tcgcaagcga ggcaagcgcc
      841 tcattatcca cgaacgacgt cttcgcaagc tccacccatg acacccgcac agaaaggccg
      901 gtcttctcga aagtatcccg cagttttgtc tccagagaaa ccatagcgga tgaatcgtaa
      961 tccttcggca tggtgatgac aagaaaaaga gcgttgtcgg cagaggaaag agagacctgc
     1021 gcgcatttga acggcttttc gcacaaagaa ttgacaaagt cgcacattcc atcctgcgaa
     1081 accaggatac tgccgaggga agttcgtttc cagaaaagat acattcgcat cacccctgtt
     1141 cgaagggggc tctgaaactg aaggagcccc cttcaaggtt cgttgatgaa ctatgaactg
     1201 aaaaaagccg catcgaagcg aatgtcagag ctgctccact tccggctgga ccgatttatc
     1261 aagctccgga acggaatcct cgggctgttt cacctcgccc ttcgcctctt cggcaacggc
     1321 ttcggcggga accttgtttt taaagtagat cccctggacg ttcacgttca ccgctttcac
     1381 cttgtagccg gtgtactctt cgatgttctt cttgatcatc tcctgaacat cccatgcaag
     1441 atcgggaatg cgaagaccga acttcacaag tatatacaca tccacgctcg ccataggatg
     1501 gagcccgtta tcctcaagca caacccgaac gccttcgctc gcttttcttc cgatgccgag
     1561 cttggaggcg ataccggggc tggcgggctg cacaccgtca atggaggaaa gcgtcttctt
     1621 cgcaagttcg gagagcacct cttcgctgat atgcactacg ccggcaacca taccctccgg
     1681 aacagcctcg ctctccccag tctcgcccaa aacgccttca tacgcagatt cttcttcaag
     1741 aacttcagat tccagctttt tgttcatttc atccatttcg agacctccct tccagtttta
     1801 cacgcccggc cggcagcgaa ttaaagctcc cggccgaatg cttttgtcta ttctttgggc
     1861 tgctcgaaat ccttcccgca gccagggcat tcaagagcct cgtccggttc gtactcctcg
     1921 ggctgatagt agaagacgta gctgcaatga gggcacgtta cggaaacgta attccggtcg
     1981 aactcgtcgt cctcgtcttc gtcatcctcg tcgtccaggt cgccttcgcc ggagaaatcg
     2041 gagaacgcct tttccagcga atcaagatct tcgtcaagaa gcgcgcagtc gtcagccaag
     2101 tcctcgtaca gctcccgctg ctcctgaatc agatcctcgt gctccgccac ctccagagag
     2161 agcgcgtcga gcgcatccac aacagccctg aaaatctttc cctgtccttc gttctcgacc
     2221 ggaccaagac cgtcgagcaa ccccttgaga taggctatct tttcccgtgc gctcatctcg
     2281 tcaacctccc tgcgttttat tttttcgccc gttcaaggta ctgacccgag cgggtgtcga
     2341 cgactattgt atcatctaca ttgacgaaaa taggaactga aatggtaatc cctgtttcga
     2401 gcttcgccgg tttgcctccg ccggacaccg tatcgccctt gaagcttgga ggcgtgtcga
     2461 cgaccttgag ttcaacgctc ttcggcatct cgatgcccat gatgcgcccc tcgaaaaact
     2521 cgatctggac ttccatatcg tccatgagga agttcacgac gttgcccaga atatccgcag
     2581 gaatagagag ctggtcatag gtcgagagat ccatgaaaat atagctgtcg ccctctttgt
     2641 aactgaactg cgcaggcttc tcgtcgaaga tgattcgctc gaagcgctcg ccggagcgga
     2701 aagagttctc gatgatcgtc cccgtgtcca ggttcttcat cttcgtcctg acggtcgctc
     2761 ctccgcgccc catcttgtga tgctggaaat caatgacttc ccatatacct tcctgccagc
     2821 ggatttttat ccccgaataa aagtccgacg tatccacaac ctgcgccata acactctacc
     2881 tcccaatatc gtttcaaaac gactaaaagc tgctgcattc accacaacgc aggtatatta
     2941 tcatatccgg aagttcttct caaaaaaacc atgaaaaaac atcgtgttac ttcaaagtga
     3001 actgctccac cggcccgtcg ggcgtcgtca gaatgtacgg aatcacgatc atcgccacct
     3061 cggacttatc tcgaacatcc ttgcggtact tgaaaagctc tccaagaaga gggatgttgc
     3121 tcaaaatcgg caccc
//
LOCUS       DAOTIL010000250         3128 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminobacteriaceae bacterium isolate AMR_MDS_3026
            NODE_36776_length_3128_cov_2.996746, whole genome shotgun sequence.
ACCESSION   DAOTIL010000250 DAOTIL010000000
VERSION     DAOTIL010000250.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868050
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminobacteriaceae bacterium (wastewater metagenome)
  ORGANISM  Aminobacteriaceae bacterium
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae.
REFERENCE   1  (bases 1 to 3128)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3128)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:19:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,224
            CDSs (total)                      :: 3,198
            Genes (coding)                    :: 3,184
            CDSs (with protein)               :: 3,184
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3128
                     /organism="Aminobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_36776_length_3128_cov_2.996746"
                     /isolate="AMR_MDS_3026"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:3034609"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(495..1652)
                     /locus_tag="P5201_06250"
     CDS             complement(495..1652)
                     /locus_tag="P5201_06250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008710812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HRV98170.1"
                     /translation="MRSMSTVRKMMALVFVLCMMAAPAFAADPIRIGEIATVTGDFAA
                     YGVAEVESVKIAVAEINAAGGILGRPVEVVMYDCRTRQEDMVNAARRLVEQDKVSVVI
                     GPSGSGLCIAAAPVFNRGQVPHIGTLPTNPLVTVDEKGAVRPYNFRICFLDPYQGRMI
                     AAFAAKELGKKKAALLYDVSSDYSHGLREFFTASFKEFGGEVIADEGHREQDVDFRAQ
                     LTVIKDANPDVLVIPTMGKALPLAVKQARELGIEVPIVGGDGYGDFMWEIVGDAMKDS
                     YWVSHVDRFDPTLADFFKKYEEQTGTQCMEFMNAVMAYDSVYWVKDAIERAGSDDPVK
                     IRDALEATKDVKLMHATLTMDEFHNPKDKDGIILKADLEKQKAVFLMKLRP"
     gene            complement(1856..3034)
                     /locus_tag="P5201_06255"
     CDS             complement(1856..3034)
                     /locus_tag="P5201_06255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008712837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="coenzyme F420-0:L-glutamate ligase"
                     /protein_id="HRV98171.1"
                     /translation="MRCTGVSARGIRLPVLSKGADLVTIVADELVKASAACRDSFEFR
                     DGDIVGVTESVLARSQGNYVTLDDIAEDVRRRVPEGDVALIFPILSRNRFGRLLEGIA
                     RGVRGTLHVVLSYPADEVGNLLIDPRAFYLKSGQLPGDLFGEKEYDEIFGRYPHPFTG
                     VDYVSYYKDMDPKKIRIHFANNPLSALRFSKTVIVASIHARFLHREILEKAGASVFSL
                     DQFCSAPLAEGKGFNEQYGLLGSNYSSETSVKLFPRESKRFARALSEELLARTGKRVE
                     VLVYGDGAFKDPAGGIWELADPVVSPGYTDGLEGLPKEVKFKYIADNAGDKSPDEAVR
                     EAIRNKGAMDRFAHSTLGTTPRRLTDLLGSLCDLVSGSGDKGTPVVYISGYFDSYLDE
                     "
BASE COUNT          663 a          927 c          831 g          707 t
ORIGIN      
        1 actcatcata agtacgcatg caagcagacg cttcatagat tcatccccct atcagttgtt
       61 cgaacatgat gaaacttctt tgtcgcagac tgtccaaccc ctccttcttt agattaaaat
      121 taacagacaa catgcagcat cacaggattc gagtagaaga gaatcctctt cttttgtgta
      181 ccgaagcact attagcgaaa aaaacggatt gcaaattcga gcgaaaaaat aaagaaggca
      241 aacaaacctg ttcgaacact ctgtgcatta ttttcttgat aaagattgtg agggaaacac
      301 ctaagaaaaa caaggttttt gtgcattgat tttattttta ttcattttgt tcattgtatt
      361 ttttgtgtat ttcactctgt acaaacaagc cgaagaaaag aaaaccacca ctggttttgt
      421 cttttcttcg gcttgtttgg ttcaactact acttgttact aatttcttcg tcttctattt
      481 tgggaaaacc cgccctacgg tctcaacttc atcaggaaaa cggccttctg tttttcaaga
      541 tccgctttca gaatgatgcc gtccttgtcc ttggggttgt ggaactcgtc catggtgagc
      601 gtcgcatgca taagcttcac atctttcgtc gcctcgagcg catctcgtat cttgacaggg
      661 tcgtcgcttc cggcgcgttc gatggcgtcc ttcacccaat acacgctgtc gtacgccata
      721 acggcgttca tgaactccat gcactgggtg ccggtctgct cctcgtactt cttgaagaag
      781 tcggcaagtg tcgggtcgaa tctgtcgacg tggctcaccc agtagctgtc tttcattgcg
      841 tcgccaacga tctcccacat gaagtcgccg taaccgtcgc cgccgacgat cggcacttcg
      901 ataccgagtt cacgggcctg cttcaccgcg agcggaagcg ccttgcccat cgtcgggatg
      961 accaggacgt cggggttggc gtccttgatg acggtaagct gtgcacggaa gtcaacatcc
     1021 tgctcccgat gtccttcgtc ggcaattacc tctccgccga actccttgaa ggaggcggtg
     1081 aagaactcgc gaagcccgtg cgaataatca ctggacacgt catagagaag cgccgctttc
     1141 ttcttgccaa gctcttttgc tgcgaatgca gcgatcatcc tcccctggta gggatcaagg
     1201 aagcagatac ggaagttgta gggcctcacg gcgcccttct catccacggt gacaagcggg
     1261 ttcgtgggga gggttccgat atggggaacc tgtccgcggt tgaagacggg agccgccgcg
     1321 atgcacaggc cgctgccgct ggggccgatg acgacgctga ccttgtcctg ttcgacaaga
     1381 cgccgggccg cgttcaccat atcctcctgc cgcgtacggc agtcgtacat aacaacctcg
     1441 accggtctgc cgaggattcc gccggcggcg ttgatctccg cgacggcgat cttcaccgac
     1501 tcaacttcag caacgccgta ggccgcgaag tcgcccgtca ccgtggcgat ctcgccgatc
     1561 ctgatcgggt ccgccgcaaa ggcgggggcc gccatcatgc acagaacaaa caccagcgcc
     1621 atcatcttcc gaaccgtact catactcctc attctttcta cctcccatac agtatgtgat
     1681 cgtgatgcac gaaacagtac cgcatttgag aaaagacggt cggatttttc cggccgccgg
     1741 atccccgcag agaaagacga cattggtccg aaattctttg tactcacaaa gtacataata
     1801 ctacatctgt gcgtttttcg caccagcctt tcggctcttt acaggctgct cgccgtcact
     1861 cgtcaagata ggaatcgaag taccccgaga tgtacacgac aggcgttccc ttgtcgccgg
     1921 agcctgaaac aaggtcgcag agcgatccca gcagatcggt caggcggcgc ggggtcgtgc
     1981 ccagcgtcga gtgggcgaag cggtccattg cgcccttgtt ccggatcgcc tcccggacgg
     2041 cttcgtcagg agacttgtcc cccgcgttgt cggctatata tttgaacttg acctccttcg
     2101 ggagcccctc cagaccgtcc gtatagccgg gggacaccac cgggtcggca agttcccaga
     2161 tgcctccggc ggggtctttg aatgcgccgt ctccgtagac gagcacctcg acgcgcttcc
     2221 ccgttctcgc caagagttcc tccgaaagcg cgcgggcaaa acgcttgctc tccctcggga
     2281 aaagcttcac cgaggtctcg ctgctgtagt tggaccccag gagaccgtac tgctcgttga
     2341 atcccttgcc ctcggcgaga ggcgcggagc agaactggtc gagtgaaaaa accgaagctc
     2401 ccgccttctc cagaatctcc cggtgcagga agcgggcgtg gatactcgcc acgatgaccg
     2461 tcttcgaaaa gcgcagcgcg gagagcggat tgttggcgaa gtgaatgcgt atcttcttcg
     2521 ggtccatgtc cttatagtag ctcacgtagt ccacgccggt gaaggggtgg gggtagcggc
     2581 cgaagatctc gtcgtactcc ttctcgccga aaaggtcgcc ggggagctgc ccgctcttca
     2641 ggtagaaagc gcgcgggtcg atgagcagat tgcccacctc gtccgcaggg taggagagga
     2701 cgacgtgcag cgtgccgcga actcctcgcg cgatgccttc gagcaggcgt ccgaaacggt
     2761 tgcggctcag aatggggaag atcagcgcga cgtccccttc gggaacccgt ctgcggacat
     2821 cctcggcgat gtcgtccaac gtgacgtagt tcccctgcga acgggcgagc accgactccg
     2881 tcacgcccac gatatcgccg tcccggaatt cgaacgaatc tcggcacgcc gcagacgcct
     2941 tgacaagttc gtcggcgacg attgtgacca gatccgcccc cttcgagagg actggaagcc
     3001 ggatacctct tgcggaaaca cctgtacagc gcataggacc tcattccttc cgtttcgact
     3061 cgccgcgcct catggaagcg gcgaacctcg aatatacgcc gggcgccatc cgcccggcat
     3121 tctgtgtt
//
LOCUS       DAOTIL010000251         3125 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminobacteriaceae bacterium isolate AMR_MDS_3026
            NODE_36818_length_3125_cov_2.916287, whole genome shotgun sequence.
ACCESSION   DAOTIL010000251 DAOTIL010000000
VERSION     DAOTIL010000251.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868050
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminobacteriaceae bacterium (wastewater metagenome)
  ORGANISM  Aminobacteriaceae bacterium
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae.
REFERENCE   1  (bases 1 to 3125)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3125)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:19:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,224
            CDSs (total)                      :: 3,198
            Genes (coding)                    :: 3,184
            CDSs (with protein)               :: 3,184
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3125
                     /organism="Aminobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_36818_length_3125_cov_2.916287"
                     /isolate="AMR_MDS_3026"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:3034609"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..280
                     /locus_tag="P5201_06260"
     CDS             <1..280
                     /locus_tag="P5201_06260"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRV98172.1"
                     /translation="IDLLLSFTDVWQPPCWEKMLGCPEPGLDEEWMDEMFGIAPGDCE
                     YHSVPYLAFLLAGTGFPFTEEELSGIRSGAGMSVGELRGNARGKEGGA"
     gene            280..2484
                     /locus_tag="P5201_06265"
     CDS             280..2484
                     /locus_tag="P5201_06265"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRV98173.1"
                     /translation="MIGGILGGTASMRLGSALRNAEMESIKGRRLLVVGVQPRLFPVA
                     GVLPEALAPGFAHNRPVDVLAESYASVQGVDAAAAFFSEAALSGQGGGVHRDSNDVYW
                     FQSMRSGLASLFRIAGLLACRKGETRSLNRILCDCFDDLYDAVSRTSPNGTPERPKWL
                     EIIPEEVRRPVQLNYLGKAGPSTYSHLSTLAVAVGELRRGAPESKTAPFAAMNDGAPL
                     FIYAPAWGNAALNILLRICMRGNYALLLPEVHLWPEAELLRVGHALRYGASGCNVVWT
                     SLSVPAHPAFDSDWEIFGKSSSSDVLGLFRARLNRYGAEGESRLKDLKYETPASLDDL
                     HALQRTPSGWSIVPCTPFDERETVELEGEITSLSKEDIFLANMAELAVFAPEPPAAIG
                     PEEPFEEEFSPKAPRGSEKPLDSRTAGGSAGDKHKSGPLSGNAPEAKVRIVEGVPETI
                     GSGKYGYCTINGETILLKDVCIDRIKYVAVLGKEVGKPQCPLAFYEKLWSAVIPPEKE
                     ILGPRVDAFVPVVVDPEKASPAGDGSMEVYSPRYGRSIRLVFHGLREMPSEKEFTAFG
                     TWKKPRSVLLVRSLLKPLAPLPPVSGKRRRATRLLTGRWKEVYSSGERLRDFMETALD
                     ELSDPSGLSDETSEKLASLRSEYEENASLAMDRLHEDGKPEKRLENRGVVCLDMETLN
                     NRDEAWEALCAADISSLPPAGYRERLKRRGLHEKYTGSLDAVFASLLLNLEL"
     gene            2560..2970
                     /locus_tag="P5201_06270"
     CDS             2560..2970
                     /locus_tag="P5201_06270"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRV98174.1"
                     /translation="MNKENTRTLFEAFPELYRGRFLDMKENLMCFGFECDDGWFELLY
                     DLSREITEHCAASGDPVPLAVQVKEKFGTLRFYADNTSEAVDDIIEKYQEKSGGICEI
                     TGAPGKLCHRRGRLKTLSEEEARKDGFCPGTKTT"
BASE COUNT          694 a          861 c          962 g          608 t
ORIGIN      
        1 gatcgacttg ctgctctcct tcaccgacgt atggcagcct ccctgctggg aaaaaatgct
       61 cggctgcccg gaaccgggtt tggacgaaga gtggatggac gaaatgttcg ggattgcccc
      121 cggagactgc gaataccatt cggttccgta tctcgctttt ctgctcgcag gaaccggttt
      181 tcccttcacc gaggaggaac tttccggtat ccggagcggc gccggaatga gcgtagggga
      241 gctgcggggg aatgcgcgcg gaaaggaggg cggagcctaa tgatcggcgg aatactggga
      301 ggtactgcct ccatgcgcct cggttcggcg ctgcgcaacg cggagatgga gtccatcaag
      361 ggacggaggc tgctcgtcgt cggcgtgcag cccaggctct tccctgtggc gggggttctg
      421 cccgaggcgc tcgcgccggg gtttgcgcac aaccgccccg tcgacgtcct tgccgagagc
      481 tacgcttccg tgcagggtgt agacgctgcg gccgcattct tctccgaagc cgctctctcc
      541 ggccaagggg gaggagtgca ccgcgattcc aacgacgtct actggttcca gagcatgcgc
      601 tccgggctgg caagcctctt ccggatcgcg gggctgctgg cgtgcaggaa aggagaaacc
      661 cgttcgctca acagaatcct gtgcgactgc ttcgacgacc tgtacgacgc ggtcagccgg
      721 acgtcgccca acggaactcc cgaacgtcct aagtggctgg aaatcatacc ggaagaagtg
      781 cgcaggccgg ttcagctgaa ctacctgggg aaggccggcc cttccaccta ttcgcacctt
      841 tcgacgctcg ccgtagcggt gggggaactc cgcagagggg ccccggaaag taaaacagcg
      901 ccgtttgccg caatgaacga cggggcgccg ctctttatct acgctcccgc ctggggaaac
      961 gctgcgctca acatcctgct tcgtatctgc atgcggggta actatgcact tcttctcccg
     1021 gaggtgcacc tctggccgga ggcggagctg cttcgcgtcg ggcacgccct caggtacggg
     1081 gcgagcggat gcaacgtcgt ctggacgtct ctctccgtac ctgcccaccc cgccttcgac
     1141 agcgactggg agatcttcgg gaagagttcg tcctccgacg tgctggggct cttccgggcg
     1201 cggctcaaca gatacggagc ggaaggggag agtcgcctca aggatctgaa atacgaaacg
     1261 ccggcgtcgt tggacgacct tcacgcgctt cagcgcactc cctccggatg gagcatcgtt
     1321 ccgtgcaccc ccttcgacga acgggaaaca gtcgaactgg agggggaaat aacctcgctt
     1381 tcaaaggaag atatttttct ggcgaacatg gccgaacttg ctgtatttgc tccggaaccc
     1441 cctgcggcaa tcggaccgga agagccgttt gaagaagagt tttcgccaaa agcgccccgg
     1501 gggagcgaga aaccgctcga ctccaggacg gcgggaggaa gcgccgggga taaacataag
     1561 agcggtcctc tttccggtaa tgcaccggag gcgaaggtcc gcatcgtcga aggagtaccg
     1621 gagacgatag gttccggaaa atacggctac tgcacaataa atggagagac tatcctactg
     1681 aaggacgttt gtatcgaccg gatcaagtac gttgccgttc tgggaaagga ggtcggaaag
     1741 ccccagtgcc cgctcgcgtt ttacgaaaaa ctctggtccg ccgttatccc gccggaaaaa
     1801 gagatcttgg gtccaagagt cgacgccttc gttcccgtcg tcgtggatcc ggaaaaagcg
     1861 agtccggctg gagacgggtc catggaggtg tattctcccc gctacgggcg gtccatccgc
     1921 cttgtgtttc atggactgag ggagatgcct tccgaaaaag agttcaccgc cttcggcacg
     1981 tggaaaaaac cgcggagcgt tcttcttgtc cgttcgttgc tgaagcctct tgcgccgctt
     2041 ccgccggtct cagggaaaag gcgccgggct acccgactgc ttacgggacg gtggaaggaa
     2101 gtgtactcaa gcggagaacg gctgcgcgac ttcatggaga cggctctcga cgaactttcg
     2161 gatccctccg ggctgtctga tgaaaccagc gaaaagctgg cctctctgcg ttcggagtac
     2221 gaggaaaacg cctcacttgc gatggatcgt ttgcacgaag atggaaagcc tgagaaacgc
     2281 ctcgaaaaca gaggagtcgt ctgtctggac atggaaacat tgaacaaccg ggatgaagcg
     2341 tgggaagcgc tctgcgcggc ggacatctcc tcacttcctc ccgccggcta cagggaacgc
     2401 ctgaaaaggc gcggactcca tgaaaagtac accgggagtc tggacgctgt ttttgcctcg
     2461 ctgctgctca atcttgagct ctaaaccatc gaaaatagcg ataaagccgt tggaaacggg
     2521 cgaagcaacc gcctatgatg aaaaacagga ggcgccgcta tgaataaaga aaatacgcgc
     2581 acactcttcg aagcttttcc ggaactctac cgtggaaggt ttctcgatat gaaggaaaac
     2641 ttgatgtgct tcggcttcga atgcgacgac ggctggtttg aacttctgta cgacctttcg
     2701 cgtgaaatta ccgaacactg tgcggcatcc ggcgatcccg tgccgttggc cgtacaggtg
     2761 aaagagaaat tcggaacgct ccgcttttat gccgacaaca cgtccgaagc agttgacgac
     2821 attatcgaga agtatcagga gaagtccggc ggaatttgcg agataacggg agcgccgggg
     2881 aagctctgtc acaggagagg gcggctgaag actctctccg aagaagaagc caggaaggac
     2941 ggcttttgcc cgggaacgaa aaccacgtag atttcttcag cgtctcaaac gcggcttgac
     3001 aagtagatat ctttaagata gtatataagt atctaaaaga ccgcaaggga gatgtgttct
     3061 atggcgcaac gaaaaaatcg gcatactgaa gctgtcggga tggaacgggc ggatgagctg
     3121 tgtac
//
LOCUS       DAOTIL010000252         3122 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminobacteriaceae bacterium isolate AMR_MDS_3026
            NODE_36851_length_3122_cov_4.418324, whole genome shotgun sequence.
ACCESSION   DAOTIL010000252 DAOTIL010000000
VERSION     DAOTIL010000252.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868050
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminobacteriaceae bacterium (wastewater metagenome)
  ORGANISM  Aminobacteriaceae bacterium
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae.
REFERENCE   1  (bases 1 to 3122)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3122)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:19:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,224
            CDSs (total)                      :: 3,198
            Genes (coding)                    :: 3,184
            CDSs (with protein)               :: 3,184
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3122
                     /organism="Aminobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_36851_length_3122_cov_4.418324"
                     /isolate="AMR_MDS_3026"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:3034609"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1453)
                     /locus_tag="P5201_06275"
     CDS             complement(<1..1453)
                     /locus_tag="P5201_06275"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HRV98175.1"
                     /translation="MKDLFERTEFLLNGGVLTLLSDSLAAHAARGVILRPEGEDLTVL
                     EAFGAGVESLFLKGRARDIQNAFPSPFLREAYDVAAAPLPGEWPWGIRLRGRDWVFFI
                     LLSEEPPKELIDHMPSFAGLLSLWQKHQKTAGTEERLSKLAYMVLATKSTLASVFEPM
                     GLDYFGAFLCDVLTESLFPSRLSVVLDKGGRLSHLDGEDIQMPPRKGIFARDILSPVP
                     VRLEESMRDSVGEAVFHDLAGEWSAILPIIGGDVRLFCLLRWETLPGEEALNFMELLG
                     NVASKALSMSAMREEKERNMQELSRRAFLLNALHEAALKFLEQTSKEELLLQILDIFS
                     EMTQAPRAVLVAWQPHAGGYVVFASKEDGILQRTGRPAGFPIGILEGDRPSSFSMEEG
                     KRLFAFMEVPFLASLDAMEGMKTIFPLWDGDRLIAFIAVSEPLPGTVTMDLETLEILA
                     RTASAAMLSREWESARLASGKYLNVAALVHWESE"
     gene            1643..2059
                     /locus_tag="P5201_06280"
     CDS             1643..2059
                     /locus_tag="P5201_06280"
                     /inference="COORDINATES: protein motif:HMM:NF018894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0035438 - cyclic-di-GMP binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilZ domain-containing protein"
                     /protein_id="HRV98176.1"
                     /translation="MHSSDTTKKQPKKIAAPGQESRRFVRVECVLRAEFRLHNHDAWL
                     PAEVLDLSVAGMKLRFNPFQRGRTLLPEVFEWADSRFRFPLKREFFHLDGHFLKIYQR
                     SAGLFTAGVEFDEPSPEDQFKLVELYAEFRRRRGAI"
     gene            2154..>3122
                     /locus_tag="P5201_06285"
     CDS             2154..>3122
                     /locus_tag="P5201_06285"
                     /inference="COORDINATES: protein motif:HMM:NF014763.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cache domain-containing protein"
                     /protein_id="HRV98177.1"
                     /translation="MKLNRLGSRILVVVLALLIVSVGGIGFYAMKIAGDNTRRMVNDY
                     EISVANGLAHELDAVFNRFDGILQALSSLVSTRFTPAMLNADVADVVVRQFASQNGKF
                     VADLGAQSVEARSLFMIFNPEIFGVKKVFSVGFQRKDQDSLYMFMDSEQFSAAKLIDR
                     KEPSTSWFWTPLDTGRPYWGDLARSETGEELVTYTMPVVIGDKIAAVAGMSFDFSFVR
                     KMLASVKIYDTGYPFLMNRDLRFLYHPSLSFDGPDLRKVSDGSMASFADAMLKEPSGR
                     FSYFYEGDEKTLAYKSLANGYIVAAAATTAESLAAVGAMQRAVYIGI"
BASE COUNT          641 a          956 c          926 g          599 t
ORIGIN      
        1 gctcgctctc ccagtgcacc agcgcggcga cgttcagata cttgccggag gcgagccggg
       61 cggactccca ctcgcggctc agcatcgcgg ccgacgcggt gcgggcgaga atctccagcg
      121 tttccagatc catggtgaca gtacccggaa gcggctccga taccgcgata aaggcgataa
      181 ggcggtcgcc gtcccagagg gggaagatcg tcttcattcc ttccattgcg tcgagcgacg
      241 cgaggaaggg gacctccata aaggcgaaaa gccgcttccc ctcctccatt gagaaggagg
      301 acgggcggtc gccttcgaga atgccgatgg ggaagcccgc cggacgcccg gtacgctgca
      361 ggatgccgtc ctccttcgag gcgaagacga cgtagccgcc cgcgtgcggc tgccaggcca
      421 caagcaccgc gcgcggagcc tgggtcatct cggagaagat gtcgagtatc tggagcagca
      481 gctcttcctt cgacgtttgt tcaaggaatt tgagggccgc ctcgtggagc gcgttcagca
      541 ggaaggctct ccgtgaaagc tcctgcatgt tccgctcctt ctcctcccgc atcgcggaca
      601 tggagagcgc cttcgatgcg acattcccca gcagctccat gaagttcagg gcttcctcgc
      661 cggggagcgt ctcccagcgg agaaggcaga agagcctgac gtccccgccg atgatcggga
      721 gaatggcgga ccactcgccc gcgaggtcgt ggaagaccgc ctctccgacc gagtcgcgca
      781 ttgactcctc cagccgcacc ggaacgggag agagaatgtc gcgcgcgaag atccctttgc
      841 ggggaggcat ctgtatatcc tcgccgtcga ggtgcgagag acgaccgcct ttatccagaa
      901 cgacggagag acgcgaagga aacaggctct ccgtaagcac gtcgcagaga aacgcgccga
      961 agtagtccag ccccatcggt tcgaagaccg acgcgagcgt gctcttggtg gccagaacca
     1021 tgtacgccag cttggaaagg cgctcctccg ttccggcggt cttctggtgt ttctgccaga
     1081 ggctgagcag tccggcgaat gaaggcatat ggtcgatcag ctctttcggc ggctcttccg
     1141 aaaggaggat gaaaaagacc cagtctctcc cgcggagacg aatcccccac ggccactccc
     1201 ccgggagggg ggccgccgcc acgtcgtacg cctcgcgaag gaagggggac ggaaacgcgt
     1261 tttgaatatc acgggctctt cctttaagga aaagactctc cacccctgcg ccgaaggctt
     1321 cgagaacggt caggtcctct ccctccggcc ggaggatcac ccctcgcgcc gcatgcgcgg
     1381 cgaggctgtc ggaaagcagc gtgaggacgc ctccgttcaa aagaaattcc gtccgttcaa
     1441 aaagatcctt catgaaccat caacatccct tcattggtgc ggatatacac gatcccgtgc
     1501 ccgggttccc ctgtcccaag ttcggggcga cgctccttct gtagtcagga agacccctct
     1561 atggtatctt aaagttgcaa aagacgccac aaaaaaatcc gaggacgccg ggggcgttct
     1621 tcgacaaaac acagggtgaa ttatgcattc ttccgatacg acgaaaaaac agcccaaaaa
     1681 aatagcggcg cccggacagg agagccgccg ttttgtccgg gtggagtgcg tcctccgcgc
     1741 ggagtttcgg ctgcacaatc atgacgcctg gctgcctgcc gaagtgctcg acctgagcgt
     1801 tgccggcatg aagctcaggt tcaacccctt ccagagaggg agaacgctgc tccccgaagt
     1861 atttgagtgg gccgacagcc gtttccgctt tcccctgaaa cgagagtttt ttcatctcga
     1921 cgggcatttt ctgaagatct accagcggag cgcaggcctc ttcacggcag gagtggagtt
     1981 cgacgaacct tcgccggagg accagttcaa gctcgtcgag ctctatgcgg agttccggcg
     2041 acggcgggga gcgatctgat tccccttctt cctttagcgc tgcgcgaggg gcgcttgctt
     2101 tatggcaata aaaagagtag cgtattctct attcttttgg aggagatgat gtgatgaaac
     2161 tgaatcggct gggttcccgc attctcgttg tcgtgctcgc gctgctgatc gtatcagtcg
     2221 gcggcatagg attttacgcc atgaagattg cgggcgacaa cacgcgccgg atggtgaacg
     2281 actatgaaat ctccgtagcc aacggacttg cgcacgaact ggacgcggtc ttcaaccgtt
     2341 tcgacggcat actgcaagcg ctttcctcgc ttgtgagcac ccggttcact ccggcgatgc
     2401 tcaacgccga cgtcgccgac gtcgtggtcc ggcagttcgc aagccagaac ggcaagttcg
     2461 tcgcagatct gggagcgcag tccgtcgaag cgcgcagcct cttcatgata ttcaaccccg
     2521 agatcttcgg cgtcaagaag gtcttcagcg tggggttcca aagaaaagac caggactcgc
     2581 tctacatgtt catggacagc gagcagtttt ccgccgccaa actcatcgac cggaaggagc
     2641 cctccacctc gtggttctgg acgccgctcg acacggggcg tccctattgg ggagatctcg
     2701 cccgctccga aacgggggag gagctcgtca cctacaccat gccggtcgtc atcggggata
     2761 aaatcgccgc cgtcgccggc atgtccttcg atttttcgtt tgtccgtaag atgctggcgt
     2821 cggtaaaaat ttacgacacg ggctacccct tcctgatgaa ccgcgatctc cgcttcctct
     2881 accacccctc gctctcgttc gacggccccg atctgcgaaa ggtatcggac ggttccatgg
     2941 cctcctttgc cgacgccatg ctgaaagagc cgagcggacg cttctcgtac ttctatgaag
     3001 gagatgaaaa aacgctggcg tacaagagcc tcgccaacgg gtacatcgtc gcggcagccg
     3061 cgacgaccgc cgagtcgctc gccgcggtcg gcgcgatgca gcgcgccgtc tacataggca
     3121 tc
//
LOCUS       DAOTIL010000253         3120 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminobacteriaceae bacterium isolate AMR_MDS_3026
            NODE_36878_length_3120_cov_5.183361, whole genome shotgun sequence.
ACCESSION   DAOTIL010000253 DAOTIL010000000
VERSION     DAOTIL010000253.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868050
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminobacteriaceae bacterium (wastewater metagenome)
  ORGANISM  Aminobacteriaceae bacterium
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae.
REFERENCE   1  (bases 1 to 3120)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3120)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:19:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,224
            CDSs (total)                      :: 3,198
            Genes (coding)                    :: 3,184
            CDSs (with protein)               :: 3,184
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3120
                     /organism="Aminobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_36878_length_3120_cov_5.183361"
                     /isolate="AMR_MDS_3026"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:3034609"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(252..2273)
                     /locus_tag="P5201_06290"
     CDS             complement(252..2273)
                     /locus_tag="P5201_06290"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:NF012707.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_process: GO:0009966 - regulation of signal transduction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HRV98178.1"
                     /translation="MTSFNSRFSRVHRIRKSLGIIFFFSFFYSLFSSSPVPSQGFYMV
                     GISSFSSASASARVVRYGMVELSPFFFKDENGNFTGYMPDLLRTLAENEGWRLSFINA
                     GGEELVKMLQEGEIDLLSMAPTPRLEGLFDFSKNHHYSTWYTFFTRSDSEILTFQDLE
                     GKTISMQSGFYALSELRRILDGIGIRYTIAEAPTVEEALEMLRSGVADVCAAEQLASL
                     AKIRRFDFRRSPIIFAPSKIFFTTTKGKNTALLARLDEKLSELNESPVPLMEVLLRRW
                     YYDDEFTFFPQWALWSFFAASILLALLSAGFVIFYKKGRELRLQGVRLQERLDSERFL
                     SDAARMLLTEGGHEDVLRNACRKIREVIDARRILLLRSTFSSTRRRSIEICFEYASPG
                     LPLLSEHSEVRSVLISDIPRKWLIDLTRKKVVKERLYETPLKKFFSVSEGAFFSFYPL
                     FEGGRFWGALAVNIDDTMESSEPKDILLSTFSEMISAHLLRKRQERRLLRIATTDALT
                     GLPNRRHFFETLDREIARSLRRGHPLSLILCDIDIFKNVNDTYGHDIGDDVLRQFARV
                     LRRGVRAEDFPVRYGGEEFGVLMPETGIRDALQTAERLRLHVEKARFDIGKGENGTVA
                     LNLTASFGVQQFAGAHDSASAFFSRADSALYEAKTGGRNRVFPVATENA"
BASE COUNT          860 a          726 c          715 g          819 t
ORIGIN      
        1 ttgagacgcc gcaatcaatc agacgaagat cctatcggca acctgcggaa caaggcagtc
       61 gccgcagtgt ataaaagaat ttaaaaaacc agtaatttag tgcgcgttgt atatttcatt
      121 tttttagcgt agtcaatctg tcgttgaagc ttgcggaggg aaatatcctc tttttctagt
      181 gataaatatt tgtttataaa tgcttttagc aagtaaaaat atccgagcgc acaatacggt
      241 cggagtccgc gttatgcatt ctccgttgct accggaaaaa cacggtttct tccccctgtt
      301 tttgcctcgt aaagagcgct atccgctctt gagaaaaaag ctgaagcact gtcatgtgcg
      361 cctgcgaact gctggactcc aaagcttgca gtcaggttga gcgcgactgt cccattctcc
      421 cccttgccaa tgtcgaaacg tgctttctcc acgtgaagac gcaggcgttc agcagtttgg
      481 agcgcgtctc ttatgccggt ttccggcata agaacaccaa actcttcgcc cccgtaccgc
      541 accgggaaat cttctgcacg cacacccctg cgaagcaccc tggcaaactg acgaagaacg
      601 tcgtcgccga tgtcgtggcc ataggtgtcg ttcacatttt tgaagatatc gatatcgcag
      661 agaatcaacg atagggggtg tccgcggcgt aaactccgcg caatttcccg atccagtgtt
      721 tcaaaaaagt gccgtcggtt cggcagtccg gtaagagcgt cggttgtcgc gatacgaaga
      781 agtcgccgtt cctggcgttt gcgcaggaga tgggccgaga tcatttcgga aaatgtcgag
      841 aggaggatat ctttcggctc ggaggattcc attgtatcgt ctatgttcac ggctaatgct
      901 ccccagaaac gtcccccttc aaaaagcggg tagaaagaga agaatgcccc ttccgaaacg
      961 gagaagaatt ttttcaatgg agtttcgtag agccgctctt ttacaacctt ctttcgtgta
     1021 aggtcaatga gccactttct aggaatatcg cttatcagga cgctccgcac ttcagagtgt
     1081 tccgaaagaa gcggtagtcc gggtgatgca tattcaaagc atatttcgat cgaacgtcgg
     1141 cgtgtagaag aaaaagtgct tcggaggaga agaattctcc tggcatcgat cacttcacgg
     1201 atttttcggc acgcgttgcg cagaacatct tcatgcccgc cttccgtaag tagcatacgc
     1261 gcggcgtcac taagaaaacg ttccgaatca agtctttctt gaagccgcac cccctgtaaa
     1321 cgaagttcac gacccttttt atagaagata acgaatccgg ctgaaagtaa tgccaagaga
     1381 attgacgctg caaagaagct ccacaatgcc cattgaggga agaaggtaaa ctcatcgtcg
     1441 tagtaccatc ggcgtaaaag gacttccatg agaggtactg gagactcgtt cagttcggaa
     1501 agtttttcgt cgagtcgagc caagagcgct gtgttttttc cttttgtagt ggtaaaaaag
     1561 attttggagg gggcaaaaat aatcgggctt ctccggaaat caaaacgtcg aatcttggcc
     1621 aggctagcaa gctgttccgc cgcgcatacg tcggcaacgc cggagcgcag catctcaagc
     1681 gcttcctcca ctgttggcgc ttccgctatc gtgtagcgga ttcctatacc gtccaggatt
     1741 cggcggagtt cggaaagcgc ataaaagccg ctttgcattg aaatagtttt gccttcaaga
     1801 tcctgaaatg tcagtatctc cgagtcagaa cgcgtgaaaa aggtgtacca cgtagaataa
     1861 tgatgattct tagagaaatc gaaaagccct tccaaacggg gcgtaggagc catactcaaa
     1921 aggtcgattt ctccctcctg aagcattttg acaagctctt caccgccggc gttaatgaag
     1981 gaaagcctcc acccctcatt ttcagcaagg gttcgcagaa gatccggcat atagcccgta
     2041 aagttcccat tctcatcctt gaaaaaaaac ggggataact ctaccattcc gtatcgaaca
     2101 acacgcgcgg aagcggaagc agaagaaaac gaagaaatgc ccaccatata aaaaccctgg
     2161 gaaggaacgg gggaagaaga aaaaagactg taaaaaaagg aaaaaaagaa gataatgcca
     2221 agagatttcc tgatacggtg tactcttgaa aaacgagagt tgaatgaggt caagaaacag
     2281 acactccctt tcggagtaaa acaaccttgc catgttttct caatatttat ttcggacccg
     2341 caatggtttt aaaatatgta ttccatgtca tcgctgtaca aagaaagcaa atagactgct
     2401 tgagaatatt ttgataacgc atcgcacgct catctgaaag aattatatga agaaaacaag
     2461 agttgcgcaa gcccaattcc aggcttacgt tgtctattaa gtgggcacta aggttcatat
     2521 atataaaagt ttatctcgtt tttgtcgcta cgtgtactaa aagtatcttt tgacattact
     2581 ttgattgtgg ggtggattct tggagtgaga acgaaaaaaa atggaagccc taatagcggt
     2641 taagctcgat tgcatcaagt tcctgcgaga ctacctccgc tttattcaga ggttccgtct
     2701 atcatcatct gtctttcctt cttgaaaacc atggaggttc tagagaggaa taacgctctt
     2761 tcattttttt cgttttctta aaacatatgg ttgtgggtag gactgcacag agaataattt
     2821 actcatctga atggctcact tttgtcaatg cttactaata tagctttccc gcactcattg
     2881 ttagcatttt tcctttttgc actccccttc tgtttgaaag caaagcttct ccgccgaaga
     2941 tgttcgtttc ccataattct ctgagggtta tcttgggttc atatcgaaaa tacttgccgg
     3001 aaagacaagt atctgagttc tgtccgcacc tccattttcc gttctttccg tcttcaaatt
     3061 tttaccagta tcgaagaacc agcaaactgc cgggcagtcg aatgtcctta cagtgcctcc
//
LOCUS       DAOTIL010000254         3116 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminobacteriaceae bacterium isolate AMR_MDS_3026
            NODE_36944_length_3116_cov_4.470761, whole genome shotgun sequence.
ACCESSION   DAOTIL010000254 DAOTIL010000000
VERSION     DAOTIL010000254.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868050
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminobacteriaceae bacterium (wastewater metagenome)
  ORGANISM  Aminobacteriaceae bacterium
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae.
REFERENCE   1  (bases 1 to 3116)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3116)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:19:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,224
            CDSs (total)                      :: 3,198
            Genes (coding)                    :: 3,184
            CDSs (with protein)               :: 3,184
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3116
                     /organism="Aminobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_36944_length_3116_cov_4.470761"
                     /isolate="AMR_MDS_3026"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:3034609"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..868
                     /locus_tag="P5201_06295"
     CDS             <1..868
                     /locus_tag="P5201_06295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014807325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="NADH-ubiquinone oxidoreductase-F iron-sulfur
                     binding region domain-containing protein"
                     /protein_id="HRV98179.1"
                     /translation="NSGLWGYPTNINNVETWANVPQIISNGGEWYASMGTEKSKGTKV
                     FALTGKVKHTGLVEVPMGITLREIVFDIGGGILNDKKFKAVQIGGPSGGCLTEEHLDT
                     PVSYESLTALGAIMGSGGLVVMDEDNCMVDVAKFFLEFTQKESCGKCVPCREGTKHML
                     LLLEKITEGKGTMEDLDTLEELAHMVRDMSLCGLGQTAPNPVLTTLRYFREEYIAHIR
                     DKRCPAGACTALLRYTILQDVCKKCGICAKNCPVHCIPGDRNSGYRIETDKCIRCGTC
                     FEKCPFGAIEKK"
     gene            888..2903
                     /locus_tag="P5201_06300"
     CDS             888..2903
                     /locus_tag="P5201_06300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013048935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HRV98180.1"
                     /translation="MSEITLVIDGVECRASKGETILQVARKNNIYIPTLCWMEGLTPI
                     GSCRMCVVEVAGNAKLLTACTTPAENNMQVSTKTEKLGKYRRQILELLFAGRNHFCMF
                     CSQTGDCELQRLAIEHGMDNVRYPFLYETFHNDASDPNIQIDHNRCILCLRCIRVCAE
                     KVGAHTLDLEKRGWGTNIMADFGRQLGESDSCVSCGACAQVCPTGTITLRDFVYRGRR
                     HDCDDQVESICPLCSLGCKIKAYVRTGSVVRVEGISGDSPDGGQLCHMGRWWLPKSTE
                     RERLSVPMIRTGSSFREASWEEALDLIAERMAPALKADRAGALVSELSTDEELTVVSS
                     FFRDTLEMKKMDGFYGNMLRGFVRGLKPFADQGVRPFTGAHNILASDAIVVVEANPQE
                     EAPVAASYIRVSTVIERAKLINVSSGKNPFDGISDQDLRTTEKNMLSLLTALRKAVEG
                     DEDGAAPEVKEIASILRNAKKPVVVIGSKLAEENPLLVTEAVNLAVASKAFFEDGLGV
                     VPMLRAGNSLGALNTITGESDWVSDGHLDFLYVLSTGMVAEDKRSLEAMTRARFVVVH
                     TPFMVHPLVNMADVLLPAPAWFERSGHYCTLEGERRRMNLVTAPTGNIKGLSVIMKEL
                     AGRLGVEMQKASAAPCENVYEAKAAPDTARTVRFAPGASVPFVKEVR"
     gene            2906..>3116
                     /locus_tag="P5201_06305"
     CDS             2906..>3116
                     /locus_tag="P5201_06305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013048934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP oxidoreductase"
                     /protein_id="HRV98181.1"
                     /translation="MAKPKIASVWLEACAGCHMSFLDIDERIVDLLDKVELTATPITD
                     IKEFPEVEVGVIEGALGNEEELHLAK"
BASE COUNT          680 a          847 c          981 g          608 t
ORIGIN      
        1 gaattccggc ctttgggggt atccgacgaa catcaacaac gtcgagacgt gggccaacgt
       61 tccgcagata atcagtaacg gcggcgagtg gtacgcctcg atgggaacgg agaagtcgaa
      121 agggacgaag gtcttcgccc tgacgggcaa ggtgaagcac accgggcttg tcgaggttcc
      181 tatgggcatt accctccggg agatcgtttt cgatatcggc ggcggcattc tcaatgacaa
      241 gaagttcaag gccgtgcaga taggcggtcc cagcggaggc tgtctgaccg aggagcatct
      301 ggacactccg gtgagctacg agtcgcttac ggcgctcggc gcgatcatgg gatcaggcgg
      361 gctggtggtc atggacgagg acaactgcat ggtcgacgtg gcgaagttct tcctcgaatt
      421 cacgcagaaa gagtcatgcg gcaagtgcgt tccctgcaga gagggaacga agcacatgct
      481 gctgcttctg gagaagatca ccgagggcaa gggaaccatg gaggatctcg acacgctgga
      541 agagcttgcg cacatggtgc gcgacatgtc cctgtgcgga ctcgggcaga ctgcgccgaa
      601 cccggtgctg acgaccctcc gctactttag ggaggagtat atcgcgcata tccgggacaa
      661 gagatgcccg gccggggcct gtaccgcgct gctccggtac actattcttc aagacgtgtg
      721 caagaagtgc ggtatctgcg ccaagaactg ccctgtccac tgtatccccg gcgaccggaa
      781 ttcggggtac cgcatagaga ccgacaagtg catccgatgc gggacgtgct tcgagaagtg
      841 ccccttcggc gccatcgaga aaaagtagtc gtggaggagg aaaactcatg agtgaaatca
      901 ccctcgtcat cgacggagtg gagtgcaggg cgtcgaaagg agagactatc ctccaggttg
      961 cccggaagaa caatatatat atcccgaccc tttgctggat ggagggactg actcctatcg
     1021 gttcctgcag aatgtgcgtc gtcgaagtgg ccggcaatgc taagctcctt accgcgtgca
     1081 caacgccggc cgagaacaac atgcaggttt cgacgaagac cgaaaagctt ggaaagtatc
     1141 gacggcagat actggagctg ctcttcgcgg ggcggaacca tttctgcatg ttctgctcgc
     1201 agactggaga ttgcgagctg cagcgccttg ccatcgaaca cggcatggac aatgtccgtt
     1261 atccgttcct gtacgagacg ttccataacg acgcttcgga cccgaacatc cagatagacc
     1321 acaaccgttg tatcctctgt cttcgctgca tcagggtctg cgccgagaag gtcggcgcgc
     1381 atacgctgga cctcgaaaag cgcggatggg ggaccaacat catggcggac ttcggcagac
     1441 agctcggcga gagcgattcc tgcgtctcgt gcggcgcgtg cgcacaggtc tgcccgaccg
     1501 ggacgatcac tcttcgcgat tttgtctacc gcggccgtcg tcatgactgc gacgaccagg
     1561 tggagagcat ctgtccgctc tgttcgctcg ggtgtaagat caaagcgtac gttcgcacgg
     1621 ggagcgtcgt ccgggtagaa ggtatctccg gcgacagtcc tgacggcgga cagctctgcc
     1681 acatggggcg ctggtggctt ccgaaatcca cggaacgcga gcgtctttcc gtccctatga
     1741 tccgtaccgg ttcctccttc cgggaggcgt cgtgggaaga agcattggat ctgatcgctg
     1801 aaagaatggc tcccgcgctg aaggccgaca gggccggagc gcttgtgagc gaactttcga
     1861 ccgacgaaga gctgacggtg gtctcatcct tcttccgcga tacgctggag atgaaaaaga
     1921 tggacggctt ctacggcaat atgctccggg gcttcgtaag agggctgaaa cccttcgccg
     1981 accagggagt ccgtccgttc acaggcgctc ataatattct ggcgagcgac gctatcgtcg
     2041 tcgtcgaggc gaacccgcag gaagaagctc cggttgctgc aagctatatt cgtgtatcga
     2101 ccgtcattga gcgagcgaag ctcatcaacg tctccagcgg aaagaatcca ttcgatggca
     2161 tttctgatca ggatctcaga acaacggaaa agaacatgct ctctcttctt accgctctgc
     2221 gcaaggccgt ggagggcgac gaggatggag cggctccgga ggtcaaggag attgcgtcca
     2281 tccttcggaa tgcgaaaaaa cccgttgttg tcatcggctc gaagcttgcc gaggagaatc
     2341 ctctgcttgt taccgaggca gtgaacctgg ctgtcgcctc gaaggcgttc ttcgaggacg
     2401 gacttggagt cgttccgatg ctgcgggccg gaaacagcct gggagcgttg aacacaatca
     2461 cgggggagag cgactgggtg agcgacggac atctcgactt cctctatgtg ctctcgacgg
     2521 ggatggtcgc ggaggacaaa cgttcgctgg aagccatgac ccgtgcgcgc ttcgtcgtgg
     2581 tgcacacgcc cttcatggtg catcctctgg tgaacatggc ggacgtgttg ctgccggctc
     2641 cggcatggtt tgaacgaagc ggtcactact gcaccctgga aggggaacgc cgcagaatga
     2701 accttgtgac cgcgcccacc ggcaatataa aagggctgtc ggttattatg aaggaactcg
     2761 ccggaaggct cggggtcgaa atgcagaagg cctccgccgc tccctgcgag aacgtctacg
     2821 aggcgaaggc tgccccggac acggcccgaa cggtccgctt cgctccggga gcttctgtac
     2881 cctttgtgaa ggaggtacga tgattatggc gaagccgaaa atcgcgtcag tatggcttga
     2941 agcctgcgcg gggtgccaca tgtcctttct cgacatagac gagcgcatcg tagaccttct
     3001 ggacaaagtg gagcttaccg ccacgcctat taccgacatc aaggagtttc cggaagtgga
     3061 ggtgggcgtt atagaaggag cgctcggcaa cgaggaggag ctgcacctgg cgaaag
//
LOCUS       DAOTIL010000255         3110 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminobacteriaceae bacterium isolate AMR_MDS_3026
            NODE_37061_length_3110_cov_2.618985, whole genome shotgun sequence.
ACCESSION   DAOTIL010000255 DAOTIL010000000
VERSION     DAOTIL010000255.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868050
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminobacteriaceae bacterium (wastewater metagenome)
  ORGANISM  Aminobacteriaceae bacterium
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae.
REFERENCE   1  (bases 1 to 3110)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3110)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:19:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,224
            CDSs (total)                      :: 3,198
            Genes (coding)                    :: 3,184
            CDSs (with protein)               :: 3,184
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3110
                     /organism="Aminobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_37061_length_3110_cov_2.618985"
                     /isolate="AMR_MDS_3026"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:3034609"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..578
                     /locus_tag="P5201_06310"
     CDS             <1..578
                     /locus_tag="P5201_06310"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRV98182.1"
                     /translation="KNLSHKLALLNIDGKKVTPAKFEIPVLWPYNVDITPDGSLALGA
                     DMGGPGGSSGHVDTVSVIDMSQNPPRVIDRVVAGDGPEGFAVSPTGEYLATVNIRGSN
                     VAHDSWFYHKNGTVSLLKIDGKKLTKVNEVEVGALPEGVVWSPDGKWLYVGNFLDKNI
                     SVLKIEDGKLVDSGVKIPVAGSPASMRGPVN"
     gene            742..2469
                     /gene="ade"
                     /locus_tag="P5201_06315"
     CDS             742..2469
                     /gene="ade"
                     /locus_tag="P5201_06315"
                     /EC_number="3.5.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013048142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000034 - adenine deaminase activity
                     [Evidence IEA];
                     GO_process: GO:0006168 - adenine salvage [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenine deaminase"
                     /protein_id="HRV98183.1"
                     /translation="MNVDDFLAVARGDREADLVLRGARVANVFTLEYEEADVAIYGGR
                     IAGVGKGYSGRVEEDLSGCVLAPGFLDGHCHIESTMLTPAGFAELAAVRGTSAVAADP
                     HEIANTCGMAGVEFMWRESLGCPVDIHYTAPSCVPASSFETPLEPLDAGAIAEMFERG
                     WCDSLGEVMNYPGVINGDPALWAKLYASGKRPRSGHAPGLSGKELCAYLLSGCDSDHE
                     SFSIEEGLEKLRRGMWLMIREGATEHNLEELAPIVKENEARSSRCMMVSDDLTARYLR
                     DVGHMDEKIRRAVRLGISPLVALRMVTLSTAEYFGWRDLGAVAPGRFADLVAVDSLEN
                     CSVRKVWKRGRLVAENGKLAGPRVSRVAPPVLAAGALSVPEESFFHLPVPAGAKIRVV
                     GLRRGQVVTEHLVMEPTVRNGMASADPERDLAYLAVLEKNRGSGRLAVGFVHGLGLKE
                     GAICSSVAHDAHNFVAAGMDIPSLRRAFALLIEQGGGIAAVRGDTVLASLALPVGGLM
                     NPSPADAVISGFEEVERAAATLGTDVNHPFMAMSFLSLSVIPELKLTDQGYVDLGRGG
                     EQSLFVEEA"
     gene            2503..>3110
                     /locus_tag="P5201_06320"
     CDS             2503..>3110
                     /locus_tag="P5201_06320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013048571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015205 - nucleobase transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NCS2 family permease"
                     /protein_id="HRV98184.1"
                     /translation="MASLLENRFHLKEAGTDVKTEVLAGITTFMTMAYIIFVNPGILQ
                     NAGMPFGALMTATCLASALATFLMAFMANYPIALAPGMGLNAFFAFSVVLGMGISWQV
                     ALAAIFVEGILFILLTLTKIRESVVNGIPKSLKIGISTGIGLFIAFIGLQSAGVIVKN
                     DAVLVGLGNLKSVEVLLACLGFFIMGALEARKVKGSILWGIL"
BASE COUNT          557 a          952 c          971 g          630 t
ORIGIN      
        1 tcaagaacct ttcgcataag ctcgcgctgc tcaacatcga cggaaagaaa gtaaccccgg
       61 cgaagttcga gatccccgtg ctctggccgt acaacgtgga catcacgccg gacggctccc
      121 tggccctggg cgccgacatg ggcggccccg gcggctcttc cggccatgtg gacaccgtct
      181 ccgtcatcga catgagccag aatccgcccc gcgtgatcga ccgtgtggtc gcgggcgacg
      241 gccccgaagg gttcgccgtc tctcccacgg gagagtacct tgcgaccgtg aacatccggg
      301 gaagcaatgt tgctcacgac tcctggttct atcacaagaa cggtaccgtc tctctgctca
      361 agatcgacgg aaagaagctc acgaaggtga acgaggtcga ggtcggcgcg ctcccagagg
      421 gcgtcgtctg gagccccgac ggcaagtggt tgtatgtcgg caacttcctc gacaagaaca
      481 tctccgtgct caagatcgag gacggaaagc tcgttgacag cggtgtgaag atacccgttg
      541 cgggatctcc ggcatccatg cgcggccctg tgaactgaag caaccgaagc atacaagccc
      601 ctgctgtttc tccaggcggg ggcttttttg tacccgtata cggatgcatt cccggattct
      661 gcaacttttc atcgcttccg attgcgcatc ggcgatcttt ccgttacact tcatcatgga
      721 aaaagaaggg aggaattgca tgtgaatgtc gatgattttc tcgccgtcgc tcggggcgac
      781 cgcgaggccg atctggtgct ccgcggagcc agggtcgcca acgtattcac cctcgaatac
      841 gaggaagcgg acgtcgccat ttacggcgga cggatcgccg gggtggggaa ggggtattcc
      901 ggacgcgtcg aggaggacct ctccggttgt gtgctcgctc ccggcttcct ggacggacac
      961 tgtcacatag agagcaccat gctcactcct gcgggcttcg ccgagctggc cgcggttcgc
     1021 ggcacgtccg ccgtcgccgc cgatccgcac gaaatagcga acacctgcgg catggccggc
     1081 gtcgaattca tgtggcgcga gagcctcggc tgcccggtgg atatccacta tacggctcct
     1141 tcatgcgttc ccgcctcgtc cttcgagacg ccgctggaac ccttggacgc gggcgctatc
     1201 gccgagatgt tcgagcgcgg ctggtgcgac tcgctgggag aagtgatgaa ctacccaggc
     1261 gtcatcaacg gcgacccggc cctttgggcc aagctgtacg cctccgggaa gcgaccccgc
     1321 tccgggcacg cccccggcct ctcgggtaag gagctgtgcg cgtacctcct ctcggggtgc
     1381 gattcggacc atgagagctt ttcaatagag gaggggctcg aaaagctccg tcgcgggatg
     1441 tggctgatga tccgcgaggg cgccacggag cacaacctgg aggagcttgc gccgatcgtc
     1501 aaagagaacg aagcgcgctc cagccgctgt atgatggtga gcgacgacct taccgcccgc
     1561 tacctccggg acgtcggtca catggacgag aagatacgcc gggcggtccg tctgggaatc
     1621 tctccgctcg tcgcgctcag gatggtcacc ctctccacgg ccgagtactt cggctggcgc
     1681 gacctggggg ccgtcgctcc cggcaggttc gccgacctcg tcgccgtgga ttcgttggag
     1741 aactgctccg tccggaaggt ctggaagcgc ggacgcctcg tcgccgagaa cggaaagctt
     1801 gccggtccca gagtttcccg cgtcgccccg cctgtgcttg ccgcaggggc tctttccgtt
     1861 ccggaggaat cttttttcca tcttcccgtc cccgcaggcg ccaaaattcg cgtcgtcgga
     1921 cttcgccggg ggcaggtggt tactgaacat ctcgtcatgg agccgaccgt gcggaacggt
     1981 atggcatcgg cggatcccga gcgtgatctg gcctacctcg ccgtcctcga aaagaaccgg
     2041 gggagcggca ggctcgctgt cggatttgtt cacggactcg ggctgaagga gggagccatc
     2101 tgctcctccg tggcgcacga cgcacacaac tttgtggcgg cgggcatgga catcccttcg
     2161 ctgcggaggg ccttcgccct ccttatcgaa cagggaggcg gcatcgccgc ggtgcggggc
     2221 gatacggtcc ttgcctctct tgcactcccc gtcggcggtt tgatgaatcc ctctccggcc
     2281 gacgcggtga tctcaggatt cgaggaggtg gaacgggccg ccgctaccct gggaaccgac
     2341 gtcaaccacc ccttcatggc gatgagcttc ctctctctct ccgtgattcc ggagctgaag
     2401 ctgaccgacc aggggtatgt cgacctcggc aggggcgggg aacaatcact ctttgttgaa
     2461 gaagcgtagt catattctca ctatagaatg ggaggaattg taatggcatc attgttggaa
     2521 aaccgctttc acctcaagga ggcggggacg gatgtgaaga cggaagtgct ggcgggtatc
     2581 accacgttca tgacgatggc gtacatcatc ttcgtgaacc ccggtatcct ccagaacgcg
     2641 ggaatgccct tcggagcgct gatgacggcg acctgcctgg cgagcgctct ggcgacgttc
     2701 ctgatggcgt tcatggcgaa ctacccgatc gcgctggcgc ccgggatggg gctcaacgcc
     2761 ttcttcgcct tcagcgtcgt ccttggcatg ggtatctcct ggcaggtcgc gcttgccgcg
     2821 atcttcgtcg aaggcattct cttcatcctg ctcacgctca cgaagatccg agagagcgtc
     2881 gtcaacggta ttcccaagtc gctcaagata ggcatctcga caggtatcgg cctttttatc
     2941 gcattcatcg gcctgcagag cgctggggtc atcgtgaaga acgacgccgt tctggtcggg
     3001 ctgggcaatt tgaagtccgt cgaagtgctg ctcgcctgcc tcggcttctt tattatggga
     3061 gcgcttgaag cgcgaaaggt gaagggctcc atcctttggg gtatccttat
//
LOCUS       DAOTIL010000256         3107 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminobacteriaceae bacterium isolate AMR_MDS_3026
            NODE_37102_length_3107_cov_3.418742, whole genome shotgun sequence.
ACCESSION   DAOTIL010000256 DAOTIL010000000
VERSION     DAOTIL010000256.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868050
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminobacteriaceae bacterium (wastewater metagenome)
  ORGANISM  Aminobacteriaceae bacterium
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae.
REFERENCE   1  (bases 1 to 3107)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3107)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:19:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,224
            CDSs (total)                      :: 3,198
            Genes (coding)                    :: 3,184
            CDSs (with protein)               :: 3,184
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3107
                     /organism="Aminobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_37102_length_3107_cov_3.418742"
                     /isolate="AMR_MDS_3026"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:3034609"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(154..375)
                     /locus_tag="P5201_06325"
     CDS             complement(154..375)
                     /locus_tag="P5201_06325"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRV98185.1"
                     /translation="MFIPLEGDGLVSIRRIVALVRHENETAVYLRDGSLLATGFKPET
                     LRKRYNAFCKEARANAEPLRAQRNGGKRT"
     gene            504..1406
                     /locus_tag="P5201_06330"
     CDS             504..1406
                     /locus_tag="P5201_06330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013049023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HRV98186.1"
                     /translation="MEIKRMRTLARRGAARVLSEIRDEWKAFLAVTIGVSIQAVAVVL
                     FVLPNRFPDLGVSGIAVLSNYVFGISPSWVILILNALLMIWAWRELSPRFVMWTMWAV
                     LLFSLLLKLFEFVPVPVIGDKFLAAMVSGVLRGLGAGLVFRVGGSTGGLDIPGVALRK
                     RYGIEMGQFSIYINMGLLGLSFFVVGLDAAIYGAVGLYVFGIVVDNTTRSFDRRKQVF
                     IITNIPEEVSAYINLSLHRGTTRFEGVGGYSKQKRPVLMTLLEPRQVIPLKRFLAEND
                     PKAFMSVSDASEVVGKGFKSWKSL"
     gene            complement(1484..2695)
                     /locus_tag="P5201_06335"
     CDS             complement(1484..2695)
                     /locus_tag="P5201_06335"
                     /inference="COORDINATES: protein motif:HMM:NF016100.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HRV98187.1"
                     /translation="MTFDVHEISPLEWDDMADGFLDLVDNEIRAAEMLLNADPPLAMP
                     SIPHSLIATEYLLFLAVQLLGASKQSWTNVDEYIKFIAEKAPDLRRTLGPLTPLKKAA
                     AAGAFDEDIFKVAESAFKATVKAEKILGEFIDKKLEELYAEHETSPASPSGGSGGRGN
                     EYGQAMEILDKAWGETSVKKRATLVKKALALCPDIADAYVILGNDALDSGKPEEAIEH
                     YRRGVEAGERELGAEAFTDDKGHFWGLLETRPYMRARFGLASALHKSGSVREASEHYK
                     AMLELNPNDNQGVRYMLIEALLLLGEHQDADALLKQYDEESAFMLYPKALLEFVKNGD
                     SKAAQKLRAEAIRNNPHVPAFLLGKKKLPKKTPDSYGFKDESEAVVYALEFGAPWSTI
                     PGAKEWLAQGV"
     gene            complement(2883..>3107)
                     /locus_tag="P5201_06340"
     CDS             complement(2883..>3107)
                     /locus_tag="P5201_06340"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRV98188.1"
                     /translation="AFPGRKQDIRLLHLREGREGERIKTPVSWNEVGGSILSAASACA
                     TGPFPAAADRCPRCFFRLDCPYRIEISTGG"
BASE COUNT          544 a          941 c          919 g          703 t
ORIGIN      
        1 ccacggagtt gtccaccact tcgtagacca ggtgatggag cccccggacg gatgtgtccc
       61 cgatgtacat tcccggccgc ttgcgaaccg cctgaagacc ttcaaggacc tggatatcct
      121 tggcggtgta ctgaagagcc gaagggctcg ttatcatgtt ctctttcctc cgtttcgttg
      181 cgcgcgaaga ggctcggcgt tcgctcttgc ctctttgcag aaagcgttgt atcttttcct
      241 cagtgtttcg ggtttgaatc cggtggcgag gagtgagccg tcgcgcaaat agaccgccgt
      301 ttcgttctca tgacgaacca gggcgactat gcggcgtatg gagacaagac cgtctccttc
      361 gagggggatg aacatgttgt ttcactccat ttcgcaagtc tttaactgaa ctattatacc
      421 agaaagccgc ggagagtgcg cggaaatgcg ctccgaatcc gttggagtcg gagaatttga
      481 aagaagcaag aggagtggtc gcaatggaaa taaaaagaat gcgcacattg gcgcgcagag
      541 gcgccgcgag agttttgagc gaaatacgcg acgagtggaa ggcgttcctc gccgtcacga
      601 taggcgtctc cattcaggcg gtcgcggtgg tgctcttcgt gctgcccaac cgcttccccg
      661 atcttggcgt ttcgggtatc gcggtgctgt cgaactacgt cttcggaatc tccccctcct
      721 gggtgatact gatactgaac gccctgctga tgatctgggc gtggagggag ctttcgccgc
      781 gcttcgtgat gtggaccatg tgggcggtgc tcctcttctc gctgctgctg aagctctttg
      841 aattcgtccc cgttcccgtg atcggcgaca aattccttgc cgccatggtc tccggcgtgc
      901 tgcgcggtct cggcgctggg ctcgtcttcc gcgtgggcgg ctccaccgga ggcctcgaca
      961 tccccggcgt cgccctgcgg aagcgctacg gcatcgagat ggggcagttc agcatctaca
     1021 tcaacatggg gcttctcggg ctctccttct tcgtcgtggg cctggacgcg gccatttacg
     1081 gcgcggtggg gttgtacgtc ttcgggatcg tcgtcgacaa cacgacgcgc tcgttcgacc
     1141 gcagaaagca ggtctttatc atcacgaaca tccccgagga agtttcggcg tacatcaacc
     1201 tctcgctgca ccgggggacg acccgtttcg agggtgtggg cgggtactcc aagcagaagc
     1261 gccccgtctt gatgactttg ctcgaaccgc gccaggtcat cccgctgaag cggtttctcg
     1321 ccgaaaacga ccccaaggcc tttatgtccg tctccgacgc ctcggaggtc gtagggaagg
     1381 ggtttaaaag ctggaagagc ctctagaccg gcaaggggtc acaaaaaagc ggaggccgga
     1441 tcacgggaaa cgccgcgtcc ggtctccggt ttttttgtgt ccgctacact ccctgcgcca
     1501 gccactcctt ggctccgggg attgtcgacc aaggagcgcc gaactcaagc gcgtagacca
     1561 cggcctcgct ctcgtctttg aaaccgtatg agtcgggcgt tttcttcggc agcttcttct
     1621 ttccgaggag gaacgccggg acgtgcggat tgttccggat cgcctcggcg cgcagctttt
     1681 gcgccgcctt cgaatcgccg ttcttcacaa attccaggag ggccttcggg tagagcatga
     1741 acgcgctctc ctcatcgtac tgcttcagga gcgcgtccgc gtcctggtgc tctccgagga
     1801 gcagcagcgc ttcgatgagc atgtagcgca ctccctggtt gtcgttcggg ttcagttcga
     1861 gcatcgcctt gtagtgttcc gaggcctcgc gtacgctgcc gcttttgtgc agcgccgagg
     1921 caagaccgaa gcgggcgcgc atgtaggggc gggtttccag aaggccccag aaatgccctt
     1981 tgtcgtcggt aaaggcttcc gcgcccagct ccctttctcc ggcctctacc cctctgcggt
     2041 agtgttcgat cgcttcttcc ggcttcccgg aatcaagagc gtcgttgccc agaatgacat
     2101 acgcgtcggc gatatcgggg cagagcgcga gcgccttttt caccagcgtt gcccgcttct
     2161 tcaccgacgt ctccccccac gccttgtcga ggatttccat cgcctgcccg tactcattcc
     2221 cgcggccgcc ggaaccgccg gagggagacg cgggcgatgt ctcatgttcg gcgtacagct
     2281 cctccagctt cttgtcgatg aattcgccga ggatcttttc ggccttcacc gtcgccttga
     2341 aggcggattc ggcgaccttg aagatgtcct cgtcgaacgc tcccgccgcc gcggccttct
     2401 tcagcggggt cagcgggccg agcgtacggc gaagatcggg cgccttctcc gcgatgaact
     2461 tgatatactc gtcgacattc gtccaggatt gcttcgaggc gcccaatagc tgtacggcca
     2521 gaaagagcag gtattccgtc gctatgagcg agtgaggtat cgagggcatg gccagaggcg
     2581 gatcagcgtt caacagcatc tccgccgcac gtatttcgtt gtcgaccagg tcgaggaatc
     2641 cgtcggccat atcgtcccat tcaagcgggc ttatctcgtg tacatcaaac gtcatctcga
     2701 tccatccctt ccttccgtct tcgactcccg agggaggatg tgcggttcgg cgcgcgcccc
     2761 gaatgccgga tcgtcccggg gaaagtccgg acgcacgcgg cgaacgaagc ggtttttcgt
     2821 tcacacatcc actcaatact aagcgtctct ttctctctcg tcaatttctt ttttgcagtt
     2881 ttttatcctc ctgtagagat ttcgatccgg tacgggcaat cgagacggaa aaaacagcgg
     2941 gggcaacggt ccgcagccgc tggaaacggc cccgtcgcgc aggctgaggc agcggacaga
     3001 atggagccgc ccacctcgtt ccacgatacc ggagtcttga tccgttctcc ttcgcgcccc
     3061 tcgcgaagat ggagcagcct gatgtcctgc ttccgcccgg ggaaggc
//
LOCUS       DAOTIL010000257         3096 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminobacteriaceae bacterium isolate AMR_MDS_3026
            NODE_37291_length_3096_cov_3.901677, whole genome shotgun sequence.
ACCESSION   DAOTIL010000257 DAOTIL010000000
VERSION     DAOTIL010000257.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868050
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminobacteriaceae bacterium (wastewater metagenome)
  ORGANISM  Aminobacteriaceae bacterium
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae.
REFERENCE   1  (bases 1 to 3096)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3096)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:19:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,224
            CDSs (total)                      :: 3,198
            Genes (coding)                    :: 3,184
            CDSs (with protein)               :: 3,184
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3096
                     /organism="Aminobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_37291_length_3096_cov_3.901677"
                     /isolate="AMR_MDS_3026"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:3034609"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(134..1324)
                     /locus_tag="P5201_06345"
     CDS             complement(134..1324)
                     /locus_tag="P5201_06345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003056109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HRV98189.1"
                     /translation="MRRGSMKGRTLLLVLLLLPLAGLFVYVMTNSGPLAPVPVTVAEV
                     ESRSISPALYGIGVVESRAVHRIGATSPGRLLWLDAQVGEMVAAGQVLGELDPVDLND
                     RIEGQRAAVRRLESLVAAADSRVAETKARKEYAEAQAARYENLLKKKAVSVEAADAKR
                     QERRTTAAAWSSARADAEAARAEQARAGSELKGLLARKEDLKLVSPVQGLLAAREAEP
                     GSTVVAGQSVFQVVETEGLRISVRFDQSMASGLRAGLPAEIVLRSRADEVFAGNILRV
                     EPIADAITEELVAKAVFEQTPGTLPPLGELAEVTVRLPALPEGPVVPDAALRRFDGRL
                     GVWMMENGSISFRPVVAGRRDLNGMVRILEGLEPGERVVVYSVKALTPRSRVKVVERL
                     GGGV"
     gene            complement(1321..1935)
                     /locus_tag="P5201_06350"
     CDS             complement(1321..1935)
                     /locus_tag="P5201_06350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012204557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HRV98190.1"
                     /translation="MAGNGKHLPAEERRAVIVETVLELAAEGNPAEITTAAIAERMRL
                     TQGALFRHFQSKDAVWSAVMEWVAERLLLLVSRAMKGASTPLAALEAAFTANVRFAAE
                     HPGAPRLLFSELQRTGESVPRDIARALMKRYAERLRLIVEEGKERGEIRNDVSSEASA
                     HLFIGVVQGLVLQSLLAGDAQAILRLAPEAFAIFRRGIEEAGRS"
     gene            2294..>3096
                     /locus_tag="P5201_06355"
     CDS             2294..>3096
                     /locus_tag="P5201_06355"
                     /inference="COORDINATES: protein motif:HMM:NF012876.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing protein"
                     /protein_id="HRV98191.1"
                     /translation="MSKSIVAKLGLLVAASMILLAASLAFLAQRGVDQMSRSVEQVAE
                     RMLNEDIKNKNANDERSAETYGKAMSGYLASIAATPLWDFDEESLQNYAKDMLNVPNV
                     AYAAIFAADGAPMAGEKKEAAHIKAFTSDILREGKTIGSVEIGLDTTYLAELTKASEQ
                     TRDQLIASFSAEASDAESAIVRRILLFAIGIVAAVLLLNVIGLLRVVAPLRLMTDVVR
                     DLGEGEGDLTVSIAVKTKDEVGRLGMSMNQFIEKLARLVIDVIGIARRVG"
BASE COUNT          621 a         1042 c          862 g          571 t
ORIGIN      
        1 accttcgcgt cgtccaccat gccgcggtac accccgccca tggtgagcgt cacgccgatg
       61 agcagcccca gtccgacgct ggtaaggacg aacttccccc atccgtggag gatatccctc
      121 ccggccaggc tgatcatacg ccgcctccca gtcgttccac gactttcact ctgcttcgcg
      181 gagtcagcgc cttcacgctg tacaccacga cgcgctcccc cggttcaagc ccttcaagaa
      241 tgcgcaccat gccgttcaag tcgcgccgac cggcaaccac cggacggaac gagatggagc
      301 cgttctccat catccagacg ccgaggcgtc cgtcgaaacg ccggagcgcc gcgtcgggga
      361 ccacaggccc ttccggcagc gcggggaggc gcaccgtcac ctcggccagc tcgccgagag
      421 ggggcagcgt tccgggagtc tgctcgaaaa cagccttcgc caccagctcc tcggtgatcg
      481 cgtccgcgat cggctcgacg cggaggatat tcccggcaaa gacctcgtcc gcacgggagc
      541 ggagaacgat ctccgcggga agtccagccc gcagtccgga agccatcgac tggtcgaacc
      601 ggacggagat cctgaggccc tccgtttcca ctacttgaaa cacgctctgt cctgcaacaa
      661 ccgtactccc cggttccgct tcgcgggccg ccagaaggcc ttgtacaggc gacacgagct
      721 tcagatcttc cttgcgggcg agcagcccct tcagctcgga tccggcgcgc gcctgctccg
      781 cccgggcggc ctccgcatcg gcgcgcgcgg acgaccacgc ggcggcggtc gtccgccgct
      841 cctggcgctt ggcgtcggca gcctcgacgc ttaccgcctt tttcttcaac aagttctcgt
      901 agcgcgcggc ctgcgcctcg gcgtactctt ttctcgcttt cgtctcggca acccgtgaat
      961 cagccgccgc aacgagagac tccagccgcc gtacggccgc cctctgcccc tctatccggt
     1021 cgttcaggtc caccggatcc agctcaccca agacctggcc cgctgcaacc atttctccga
     1081 cctgcgcatc aagccagagc agcctccccg gagatgtcgc gcctattctg tgcaccgcac
     1141 gggactccac gacgccgata ccgtagagcg ccggggagat cgatctgctt tcgacctcgg
     1201 ccaccgtcac ggggaccggg gcaagaggcc cggagttcgt catcacatag acaaagagcc
     1261 ccgcaagagg aagcagcagc agaacaagca gcaacgtccg tcccttcatc gatcctcttc
     1321 tcatgagcgt cccgcctcct ctatgcctct gcggaaaatc gcaaacgctt cgggagcaag
     1381 ccggagtatc gcctgcgcgt cccccgcgag aagcgactgg agcacaagcc cctggacgac
     1441 gccgataaag agatgcgccg aggcttcgga agagacgtca ttacggatct cccctctttc
     1501 cttcccctct tcgacgatca gccggagacg ctcggcgtac cgtttcatca gcgccctggc
     1561 gatatcgcgg ggaacgctct cgcccgtgcg ctgcagctcg ctgaagagca gcctcggcgc
     1621 tcccggatgc tccgcggcga agcgaacatt cgcagtgaag gcggcctcca gggcggcaag
     1681 aggcgttgac gcgcctttca tcgcccgcga aaccaagaga agcaggcgct ccgctaccca
     1741 ctccatgact gcgctccaca cagcatcctt gctctgaaaa tggcgaaaca gcgccccctg
     1801 cgtcagccgc attctctcgg cgatggccgc agtggtgatc tcggcgggat tgccctcggc
     1861 cgccagttca agcaccgttt cgacgataac cgcgcgccgt tcctcggcag gaagatgttt
     1921 tccgttgccc gccatgctga tcccctctct cgtttcaaaa gtaagtaatt gattactatc
     1981 ttatgcgccg gcattcattc tgtcaagccc ctgacacgct ctgcctgctc catatttact
     2041 gtacattgga gaaaaagcgg aacgaggcaa agacaaagct tgcagcaaag agaatttaag
     2101 tgtattcttt aaattggggg aagtacttgg tacaactctc atagaaggat acaaagctca
     2161 ttacaatttc tttttccttt ctctcaaagt acgccaagac gtattccctg cccgggaata
     2221 ccttttcccg cagcacgttc gatgccgtcg gacactttcc gtcgcgtcaa aaattctcga
     2281 gggggtaatt cgtgtgtcga aaagcatcgt ggcaaagctc ggtttactgg tggcggcgag
     2341 catgattctt ctggccgctt cgctcgcctt cctggcgcaa agaggagtgg accagatgtc
     2401 gcgttcggtt gaacaggttg ccgaacggat gctcaacgag gacatcaaga acaagaacgc
     2461 gaacgatgaa cgaagcgccg agacctatgg aaaggccatg tccggctatc ttgcctccat
     2521 agccgccacg cctctttggg acttcgacga ggagtcgctc cagaactacg cgaaggatat
     2581 gctcaacgta ccgaacgtcg cctatgcggc catcttcgcc gcagacggcg ctcccatggc
     2641 cggcgagaag aaggaggcgg cgcacatcaa ggcgttcaca tccgacatcc tgcgtgaagg
     2701 gaagacgata ggttcggtgg agataggact cgacacgacc taccttgcgg aactcacgaa
     2761 ggccagcgaa cagaccaggg accagctcat cgcctccttc tcggccgaag catccgacgc
     2821 cgaaagcgct atcgtcaggc gcatcctcct cttcgccatc ggcatcgtcg ccgcggtgct
     2881 cctcctcaat gtcatcggac tccttcgggt ggtcgccccg ctgcgcttga tgaccgacgt
     2941 ggtccgcgac ctcggagagg gagagggcga ccttaccgtc tcgatcgccg tcaagacgaa
     3001 agacgaagtg ggacgactgg gcatgtccat gaatcagttc atcgagaaac tcgcccgcct
     3061 ggtgatcgac gtgatcggaa tagcccgcag ggtcgg
//
LOCUS       DAOTIL010000258         3092 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminobacteriaceae bacterium isolate AMR_MDS_3026
            NODE_37355_length_3092_cov_3.171880, whole genome shotgun sequence.
ACCESSION   DAOTIL010000258 DAOTIL010000000
VERSION     DAOTIL010000258.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868050
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminobacteriaceae bacterium (wastewater metagenome)
  ORGANISM  Aminobacteriaceae bacterium
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae.
REFERENCE   1  (bases 1 to 3092)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3092)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:19:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,224
            CDSs (total)                      :: 3,198
            Genes (coding)                    :: 3,184
            CDSs (with protein)               :: 3,184
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3092
                     /organism="Aminobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_37355_length_3092_cov_3.171880"
                     /isolate="AMR_MDS_3026"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:3034609"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..554)
                     /locus_tag="P5201_06360"
     CDS             complement(<1..554)
                     /locus_tag="P5201_06360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006300624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="J domain-containing protein"
                     /protein_id="HRV98192.1"
                     /translation="MYSGGETDDLYQILGVPRDASAADIKKAYRQLVRKYHPDANPGN
                     KDAEERFKKINMAYEVLSDSQKRTQYDQFGTVGDMPGGGSPFEGFGGMGDIFGDIFDS
                     VFGGGMGRRRANPNAPRRGADLEMEVEVTLLEAATGAAKDFQIPRWESCGACGGTGAK
                     AGTSPESCSQCGGRGQVESRQQTP"
     gene            complement(570..2387)
                     /gene="dnaK"
                     /locus_tag="P5201_06365"
     CDS             complement(570..2387)
                     /gene="dnaK"
                     /locus_tag="P5201_06365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015555922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaK"
                     /protein_id="HRV98193.1"
                     /translation="MSKVVGIDLGTTNSCIAVQEGDVTTIIPNAEGNRTTPSVVAFTK
                     EGERLVGQLAKRQAIVNGDRTIMSIKREMGTDHRVTVDGKKYTPQEISSMILQKMKRD
                     AEDYLGEPVTKAVITVPAYFTDAQRQATKDAGAIAGLEVLRIINEPTAACLAYGENKQ
                     GEHKILVFDLGGGTFDVSILDVGEGVFEVLATAGDNRLGGDDWDMRVVDWMVEQFRKS
                     EGIDLKNDRMALQRLREAAEKAKVELSSMPETTISLPFITANASGPKHLEMSLSRARF
                     DEMTADLLERTVKPTRQAIQDSGLSAGEIDKILLVGGSTRMPMVQRKIKELLGKEPTK
                     GINPDECVAAGAAIQGAILAGDHKDIVLVDVTPLSLGLETLGGVFTKIIERNTAIPVS
                     KSQTFTTAGNNQTQVEISVLQGERAMAADNVKLGKFVLDGIPPAPRGIPQIEVTFNID
                     VNGILNVSAKDKGTGKAQNISIHSSNLSKDDIERMKNQAEEFEEEDKKKRETAETKNE
                     ADGAAYAAERLLSENGDKMTPEEKGKITSGMEELRRLLSGEDLASIRSAKESLEKALQ
                     EFSTRLYSQAQQQDDSSGTAGSASDGDTVDAEFSDQGQA"
     gene            complement(2535..>3092)
                     /locus_tag="P5201_06370"
     CDS             complement(2535..>3092)
                     /locus_tag="P5201_06370"
                     /inference="COORDINATES: protein motif:HMM:NF013212.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000774 - adenyl-nucleotide exchange
                     factor activity [Evidence IEA];
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA];
                     GO_function: GO:0051087 - chaperone binding [Evidence
                     IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide exchange factor GrpE"
                     /protein_id="HRV98194.1"
                     /translation="VASASTEESVSRDEPEARQEVAPSPEELLAALQNAEREKIELTE
                     ALARARADFFNYRKRVDRDRQKERSMIAEEKALDFLPVLDNLDRALSVSPDIDGKSVL
                     QGVAMVRKQFLAVLESMGVEAVPSIGSVFSPEYHEAVVAVATDDPEKNGIVMEEIQSG
                     YRTKERVLRPAKVKVASYTLTAEKG"
BASE COUNT          536 a         1004 c          845 g          707 t
ORIGIN      
        1 aaaggcgtct gctgcctgct ctccacctgt ccgcgcccgc cgcactgtga gcagctctcg
       61 ggcgacgtac cggccttcgc gcctgtcccg ccgcacgcgc cgcacgactc ccagcgcgga
      121 atctgaaagt ctttcgccgc cccggtcgcc gcctccaaca gcgtgacttc gacctccatc
      181 tccaggtcgg ccccccgtcg gggagcgttt gggttcgctc ttcgccgccc cattccaccg
      241 ccgaagacac tgtcgaatat atcgccgaag atgtccccca tgccgccgaa tccttcgaac
      301 ggactccctc cgcccggcat atcgcccacg gttccgaact gatcgtactg ggtccgcttc
      361 tgggagtcgc tcagcacttc gtacgccata ttgatcttct tgaaacgctc ctcggcatct
      421 ttgttccccg gattggcgtc ggggtggtac tttcgtacca gctggcggta ggctttcttt
      481 atgtcagcgg cggaagcgtc ccggggcacc cccagtattt gatacaggtc gtcggtctct
      541 cctccgctgt acatacacgg tcacctcccc tacgcctggc cctggtcgct gaactcagcg
      601 tccacggtgt cgccgtcgga ggcgctcccc gccgttccgg aagagtcgtc ctgctgctgc
      661 gcctgagaat agagcctggt ggagaactcc tggagcgcct tttcgaggct ctccttcgcc
      721 gagcggatgg aggcgagatc ttcaccggag agaagcctgc ggagctcctc cattcccgac
      781 gtgattttcc ccttctcttc aggggtcatc ttgtctccgt tctccgagag aaggcgctcc
      841 gccgcgtatg cggccccgtc cgcttcgttc ttcgtctcgg ccgtctcacg cttcttcttg
      901 tcctcttctt caaactcctc cgcctgattc ttcatccgct cgatgtcgtc cttcgagagg
      961 ttcgacgagt ggatggagat attctgcgcc ttgcccgtcc ccttgtcctt cgcgcttacg
     1021 ttgagaatac cgttgacgtc gatattgaag gtgacctcta tctgcggaat gccgcggggc
     1081 gccggaggaa tcccgtcgag gacgaatttg cccagcttca cgttgtcggc ggccatggct
     1141 cgttctccct ggagcacgct gatctcgacc tgggtctgat tgttccccgc agtggtgaac
     1201 gtctggctct tcgagacagg gatggcggtg ttgcgttcaa taatcttcgt gaacacgccg
     1261 ccgagcgtct ccaatccgag ggagagaggc gttacgtcaa cgagaacaat atccttgtgg
     1321 tcgcccgcga gaatggctcc ctgaattgca gcgcccgccg caacgcactc gtcggggttg
     1381 atgccctttg taggctcctt cccgagaagc tccttgatct tccgctgcac catcggcata
     1441 cgagtggaac cgccgaccag aaggatcttg tcgatctccc cggcggacag gccggagtcc
     1501 tgaattgcct ggcgcgtggg tttcaccgta cgctccagaa gatcggccgt catctcatcg
     1561 aacctggctc tggagaggct catctcaagg tgcttggggc cggaggcgtt cgctgtgatg
     1621 aaaggaagcg aaatcgtcgt ctcgggcatc gaggagagct cgaccttcgc cttctccgca
     1681 gcctctcgga gccgctggag cgccatacga tcattcttca ggtcgatccc ctcggatttg
     1741 cggaactgct ccaccatcca gtcgacaacc cgcatatccc agtcgtcgcc gccaaggcgg
     1801 ttgtcgccgg cggtggcgag cacctcaaac acgccctcgc caacgtcgag gatcgacacg
     1861 tcgaaagttc cgccgccgag gtcgaataca aggatcttat gctcgccctg cttgttctct
     1921 ccgtaggcga ggcatgctgc cgtcggctcg ttgataatgc ggagcacttc aagcccggcg
     1981 atcgcccccg cgtccttcgt cgcctggcgc tgcgcgtcgg tgaaatacgc gggaaccgtg
     2041 atcacagcct tcgtcaccgg ctcgccgaga tagtcctccg cgtcgcgctt catcttttgc
     2101 aggatcatcg aagagatctc ctggggagta tactttttcc cgtcgacggt aactctgtgg
     2161 tcagttccca tctcacgctt tatggacatg atcgtcctgt cgccgttcac gatagcctgg
     2221 cgcttggcga gctggccgac aagacgctct ccttccttcg taaacgcgac gacggacggg
     2281 gttgtgcggt tcccttcagc attggggatt attgttgtaa cgtccccctc ctgaacagcg
     2341 atgcagctgt tggttgttcc aagatcgatt cctaccactt tcgacatatt tttctcacct
     2401 cgtaatagtt tgaacagaag atcgtactcc gcacaagaca tggcaaaaga gtgcacacgc
     2461 tgtaagagct cgctctgaat gtactcgggg ggaccgcccc ctgcgggaaa ccggcagaga
     2521 aagaagcgga cgcgctaccc cttttccgcc gtaagcgtat aagaagctac cttcaccttc
     2581 gccggacgga gcacgcgttc cttcgtccga taaccggact gtatctcctc catgacgatt
     2641 ccgttttttt ccgggtcatc agtcgcaacc gcaactactg cttcgtggta ctccggcgag
     2701 aagacagagc ctatagacgg gacagcctcg acgcccatcg attccagaac tgcgaggaac
     2761 tgtttcctga ccatggcgac gccttgaagc acgctcttcc cgtcgatgtc cggggacact
     2821 gaaagcgcgc ggtccagatt gtcgaggacc ggcagaaaat caagcgcctt ctcttcggcg
     2881 atcatcgacc gctctttctg acggtctctg tcgacccgtt tgcgatagtt gaaaaaatcc
     2941 gcgcgcgctc tggccaaggc ctccgtcagt tcaattttct cacgctccgc attctgaagc
     3001 gcagcaagca gctcttccgg tgaaggcgca acctcctgac gagcttccgg ttcgtcccgg
     3061 gagacgctct cttcggttga agcggaggcg ac
//
LOCUS       DAOTIL010000259         3091 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminobacteriaceae bacterium isolate AMR_MDS_3026
            NODE_37372_length_3091_cov_3.418972, whole genome shotgun sequence.
ACCESSION   DAOTIL010000259 DAOTIL010000000
VERSION     DAOTIL010000259.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868050
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminobacteriaceae bacterium (wastewater metagenome)
  ORGANISM  Aminobacteriaceae bacterium
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae.
REFERENCE   1  (bases 1 to 3091)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3091)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:19:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,224
            CDSs (total)                      :: 3,198
            Genes (coding)                    :: 3,184
            CDSs (with protein)               :: 3,184
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3091
                     /organism="Aminobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_37372_length_3091_cov_3.418972"
                     /isolate="AMR_MDS_3026"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:3034609"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1176
                     /locus_tag="P5201_06375"
     CDS             <1..1176
                     /locus_tag="P5201_06375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008708877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0031317 - tripartite ATP-independent
                     periplasmic transporter complex [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter large permease"
                     /protein_id="HRV98195.1"
                     /translation="PLMAIVQQMVQGVNSFSLLAIPFFIIAGEMMGEGGISRRLIAFS
                     NLLVGRVRGGLAQVNVLASMFFGGISGSAVADVSSIGTILIPMMKEKGYDADYSVAVT
                     VTSACQGIIIPPSHNMIIYSLAAGGVSVGRLFLGGFVPGVLLGIVLMIISYIIAVQRA
                     YPKEKPYTFREALQVSKDAVLGIFTAVIIIGGVISGVFTATESAAVACVYAFFVTFFI
                     YREIPLSRMNKILYSSLKTLAMVMSLIAAAKAFGWLLAYLKIPGMATSLLLSVTDNPI
                     LLLLLVNLLLLGLGCIMDMAPLILITTPILFPVVVGTLGMHPVQFGIMMMLNLGIGLC
                     TPPVGSALFVGCAVGKISIEKATKAMLPFYAAMIVVLLLVTFIPQLVLFIPNMLMGN"
     gene            complement(1280..2578)
                     /locus_tag="P5201_06380"
     CDS             complement(1280..2578)
                     /locus_tag="P5201_06380"
                     /EC_number="1.1.1.58"
                     /inference="COORDINATES: protein motif:HMM:NF019736.2"
                     /note="catalyzes the formation of D-tagaturonate from
                     D-altronate; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagaturonate reductase"
                     /protein_id="HRV98196.1"
                     /translation="MNYTVMQFGEGNFIRSFFDWMLKRITDATGEEHKVFLVQPISNG
                     RVKEIVEAGEYHVLLKGFEAGEYKEILESVPVIAGGVNPFERGGMKALLDAALSPELR
                     VVTSNTTEAGIFFEEKMEPHNFPSLLAAVLRARAKEGAPPLHILPLELIESNGTALKK
                     CVEQYGRLWGCEPVFFSYLDSCTFYDTLVDRIVPGFPAKDAPEIFRRLGKEDHNLAMG
                     ELFHLFVIQGDKAILDVLPFHKAGLNVIVTADKLPFYRDRKVRILNGVHTASVPVGLL
                     AGVELVKDFVKDERFAPMLRRLVHDEIVPAFSDDQEAHRYGDDVLERFSNPALEHAFR
                     SIALNSVAKSNTRLRPTLEGYFEKFGELPQTLVECIAAMFSFYDCDGVRELPKGPFVL
                     ADFSRIKGRTVSKMVDSFFPGLDLSLREALIRKLEYARKQ"
     gene            complement(2654..>3091)
                     /locus_tag="P5201_06385"
     CDS             complement(2654..>3091)
                     /locus_tag="P5201_06385"
                     /inference="COORDINATES: protein motif:HMM:NF016203.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UxaA family hydrolase"
                     /protein_id="HRV98197.1"
                     /translation="GITTLEEKSLGAVNKCGSGAVTDVLAMGEQAKRPGVSVVFGPGN
                     DLVSSTALAAGGAQIILFTTGRGTPFSTVVPTIKISSNTRLFGRKPRWIDFDAGPLLS
                     GTPRHRLAEDLTGLVLEVASGRRTRNEENRSGEIAIFKDGVTL"
BASE COUNT          593 a          976 c          847 g          675 t
ORIGIN      
        1 cctctcatgg ccatcgtcca gcagatggtg cagggagtga actctttctc tctgctcgcg
       61 attccctttt ttatcatcgc cggcgagatg atgggcgagg gaggtatttc acggcggctc
      121 atcgcattct cgaacctgct cgtagggcgc gtccggggcg ggctcgccca ggtcaacgtg
      181 ctggcgagca tgttcttcgg cggcatctcc ggttccgccg tggcggacgt ctcctccatc
      241 ggcaccattc tcatcccgat gatgaaggag aaggggtacg acgcggacta ctccgtcgcg
      301 gtgacggtga cgagcgcgtg ccagggcatc atcatcccgc cgagccacaa catgatcatc
      361 tactcgctgg cggcgggagg cgtttcggtt ggccgccttt tcctcggagg cttcgtcccg
      421 ggcgtactgc tcggaatcgt tcttatgatt atcagctaca taatcgcagt acaacgggcg
      481 tacccgaagg aaaagcccta cacgtttcgt gaagctcttc aagtttccaa ggatgccgta
      541 cttggcatct tcaccgccgt catcatcatc ggcggcgtca tctcgggcgt cttcacggcc
      601 acggagtcgg cagccgtcgc gtgcgtctac gccttcttcg tcacattctt catctacagg
      661 gaaatcccgc tgagcagaat gaacaagatc ctctactcct ccctgaagac cctcgcgatg
      721 gtgatgagcc tgatcgccgc ggcgaaggcc ttcggctggc tgctcgccta tctgaagatc
      781 cccggcatgg cgaccagcct ccttctttcc gtcacggaca accctattct tttacttctt
      841 ctcgtcaatc tgctccttct cgggctcggc tgcatcatgg acatggcgcc gctcattctc
      901 atcacaacgc ccattctctt ccccgtcgtc gtgggtactc tggggatgca cccggtccag
      961 ttcggcatca tgatgatgct caacctcggc atcggactct gcacgccgcc agttggctcc
     1021 gccctcttcg tgggctgcgc ggtgggaaag atctccatcg aaaaagcgac aaaggcgatg
     1081 ctccccttct acgctgcaat gattgttgtt ttgctcctcg tcacctttat cccgcagctc
     1141 gtcctcttca tcccgaacat gctgatggga aactgacggc tctgtttctc actggaaaca
     1201 caaaaaacaa cacagaggac cggggagttt ctctccggtc ctctgtgtat cctgctccgt
     1261 tcacgcacgc tgcggcgcgt tattgtttcc tggcgtactc cagcttccgg ataagcgctt
     1321 cccgcagcga aaggtccagc ccgggaaaga agctgtcgac catcttcgag acggttctcc
     1381 ccttgattcg tgagaaatcc gccaggacga aggggccttt cggcaactcc ctgacgccgt
     1441 cgcaatcgta gaatgagaac atcgccgcaa tacactccac aagcgtctgc ggcagctcgc
     1501 cgaacttctc gaaataccct tccagcgtag ggcggagacg ggtgttgctc ttcgcgacgg
     1561 agttcagcgc gatggagcgg aaggcatgtt ccagcgcggg gttgctgaag cgctcaagga
     1621 cgtcgtcgcc gtagcggtgc gcctcctggt cgtcggagaa cgcgggaacg atctcgtcat
     1681 ggacgagccg acgcagcatc ggcgcgaagc gttcgtcctt cacgaagtct ttgacgagtt
     1741 ccacacccgc gagaagaccg accggaacgg acgccgtgtg caccccgttg agaatccgca
     1801 cctttcgatc tctgtagaac ggaagtttgt cggcggtgac gatcacattc aatcccgcct
     1861 tgtggaaggg caggacatcg agaatcgcct tgtccccctg gatcacgaac agatgaaaga
     1921 gctcccccat ggcgaggttg tgatcctctt tccccagacg ccggaagatc tctggagcgt
     1981 ccttggccgg gaagcccggg acgatacgat ccaccagcgt atcgtagaag gtacaggagt
     2041 cgagatagga gaagaatacc ggttcgcacc cccagagacg tccgtactgc tccacgcact
     2101 ttttcaaagc cgttccgtta ctctcgataa gctccaacgg aaggatatgc agcggaggcg
     2161 ccccctcctt tgcgcgggcg cgcagaactg cggccagcag cgacggaaag ttgtgcggct
     2221 ccatcttctc ttcaaagaag atcccggcct cggtggtatt cgacgtcacc acccgaagct
     2281 ccggagaaag cgcggcgtcg agcaacgctt tcattccgcc gcgttcgaag gggttgacgc
     2341 cccccgcgat gaccgggacg gactcaagaa tctctttata ctcccccgcc tcgaatcctt
     2401 tcagaagaac gtggtattct cccgcctcga cgatctcttt cactcttccg ttggaaatag
     2461 gctgcacaag aaagaccttg tgctcttcgc ccgtcgcgtc ggtaatgcgc ttgagcatcc
     2521 agtcgaaaaa cgaccgtatg aagttcccct cgccgaactg catcactgta tagttcatcg
     2581 tatcctctcc ttcgtcgcga gcatttctgt gcatcttggt ttcgcacggc gtcctcgttg
     2641 aaatcggggc ctcttagagc gtcacgccgt ctttgaatat ggcgatctcc ccggagcggt
     2701 tctcctcgtt tcgggtacgg cgccccgacg caacttcaag cacaagaccc gtcaagtctt
     2761 cagcgagacg gtgtctcggc gtcccggaga gcagcggccc ggcgtcgaaa tcgatccagc
     2821 gcggtttccg cccgaaaagg cgcgtgttcg aggagatctt tatcgtcgga actaccgttg
     2881 agaacggcgt cccgcgtccc gtagtaaaaa ggatgatctg tgcgcccccg gcggccagag
     2941 cagtcgacga cacaaggtcg tttcccgggc cgaacacgac gctcacaccg gggcgcttcg
     3001 cctgttcgcc catggcgagg acgtctgtta ccgcaccgga accgcacttg ttcaccgcgc
     3061 cgagcgactt ctcctccaac gtagtgatgc c
//
LOCUS       DAOTIL010000260         3082 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminobacteriaceae bacterium isolate AMR_MDS_3026
            NODE_37514_length_3082_cov_3.509085, whole genome shotgun sequence.
ACCESSION   DAOTIL010000260 DAOTIL010000000
VERSION     DAOTIL010000260.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868050
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminobacteriaceae bacterium (wastewater metagenome)
  ORGANISM  Aminobacteriaceae bacterium
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae.
REFERENCE   1  (bases 1 to 3082)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3082)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:19:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,224
            CDSs (total)                      :: 3,198
            Genes (coding)                    :: 3,184
            CDSs (with protein)               :: 3,184
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3082
                     /organism="Aminobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_37514_length_3082_cov_3.509085"
                     /isolate="AMR_MDS_3026"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:3034609"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            63..836
                     /locus_tag="P5201_06390"
     CDS             63..836
                     /locus_tag="P5201_06390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013047571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HRV98198.1"
                     /translation="MSAIFELSGVTKNFGGVRAVSEMSFSIPEGELAGLMGPNGAGKT
                     TIFNLVTGVYDPDEGSILFRGENLVPLKTFDIVHKGIARTFQNLRLFQRSSVLENVMT
                     AAQQHVPYTFFEGMTHLGRWRSKESDIRKKSMELLERVGLADRANQAAGTLPYGHQRR
                     LEIARALALDPKLLLLDEPAAGMNAEEVLSLNSLIRSIHTDFKLTILVIEHHMDLIME
                     ICPHIVCMNFGAKIAEGTPDEIQNHPEVLKAYLGEEEEE"
     gene            833..1603
                     /locus_tag="P5201_06395"
     CDS             833..1603
                     /locus_tag="P5201_06395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005659995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HRV98199.1"
                     /translation="MSDAVMNEKKALLEVHDLHVSYGAIKALSGVSLKVFEGEIVSVI
                     GSNGAGKSTLMNAIMGMVRREKGEILLGDVKLDSRSFRVVQQGISLSPEGRKVFAPLT
                     VLENLKMGAFPRGNDSKVEEDYEWVFSLFPRLKERISQYAGTLSGGEQQMLAIGRALM
                     SRPKVLLLDEPSLGLAPIIIKDIFKELRRINNEGVTILLVEQNARQALLLSNRGYVIQ
                     TGRLVTEGPSEELLANADIKAAYLGGGHAKGKTTQALA"
     gene            1734..2195
                     /locus_tag="P5201_06400"
     CDS             1734..2195
                     /locus_tag="P5201_06400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013047575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rrf2 family transcriptional regulator"
                     /protein_id="HRV98200.1"
                     /translation="MAIKLSTKTRYGLRALLYMAERYDGERTVPVGEIAKEQEVSEKY
                     LEQLFLKLRRSNLIQSVRGAQGGYMLGRPPEDITIACVVDSLEGDITFADCLSPGGCR
                     NQGSCPTHELWSRLKGSIDEILEDTTLADLLKNSATNHAGEKSGKGSCSSK"
     gene            2212..>3082
                     /locus_tag="P5201_06405"
     CDS             2212..>3082
                     /locus_tag="P5201_06405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005661575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HRV98201.1"
                     /translation="MSRQVYLDYAATTFTAPEVVSAMTPYFTQSFGNPSSIYSFSESN
                     RKAMGIARQQVADVLGAAKEEIFFTSGGTEADNWALKGVAWRNRSKGNHVITTKIEHH
                     AVLHTAQFLQENGFEVTYLDVDAEGRVRLEDLKNAVTEKTILVSVMFANNEIGTIQPI
                     AEIGAFCREKGIIFHTDAVQAVAHVPIDVKAMNIDLLSLSAHKFYGPKGVGALYVRKG
                     IKIDNFMHGGGQERGKRATTENVAGIVGLGAAIELTASRMKDASSRVGKLRNMLIDGV
                     MKVIPHAKLNGAPV"
BASE COUNT          743 a          792 c          899 g          648 t
ORIGIN      
        1 ggtatcatgg gcaaccgtga gcttctctcc tggatcaagg atcctttcgg gaggcggtcg
       61 tgatgagcgc gatcttcgaa ctttccggcg ttacaaagaa cttcggcggc gtgcgcgctg
      121 tgagcgaaat gtccttttca attccggagg gcgagctggc aggccttatg ggaccgaacg
      181 gagcgggcaa gactacgatc ttcaatctag taaccggcgt ctacgatccc gacgaggggt
      241 cgattctctt ccgcggtgag aacctggttc ctctgaagac gttcgacatt gtccacaaag
      301 ggattgccag aacctttcag aacctgcggc tgtttcagcg ttcctcggtt ctggagaatg
      361 tcatgaccgc tgcgcagcag catgtcccct acaccttctt cgagggaatg acgcacctcg
      421 gacggtggcg gagcaaagag agcgacatac ggaagaagag catggaactg ctcgaaaggg
      481 ttggactcgc cgacagagcg aaccaggctg cggggacgct cccgtacgga caccagcggc
      541 gtctggagat tgcccgtgcg ctcgcgctgg acccgaaatt gctgctgctt gacgagcctg
      601 cggccggaat gaacgccgaa gaggttctct cgctgaactc tttgatccgg tccattcaca
      661 cggatttcaa actgacgatc ctcgtcatag aacatcacat ggatctcatc atggagatct
      721 gcccccatat cgtgtgtatg aacttcggcg cgaagatcgc cgaaggaacg ccggatgaga
      781 tccagaacca tccggaagtt ctcaaggcgt atctcggaga ggaggaagag gaatgagcga
      841 cgcggttatg aacgagaaaa aagccctcct cgaagtccat gacctccacg tgagctacgg
      901 cgccatcaag gctctctccg gagtctctct gaaggtgttc gaaggcgaga tcgtcagcgt
      961 catcggttcg aacggcgcgg gcaaatctac actgatgaat gctatcatgg gtatggtgcg
     1021 gcgagaaaaa ggagaaatcc ttcttggcga cgtaaagctt gactcgagaa gtttcagggt
     1081 ggtacagcag gggatttcgc tcagccccga gggccgcaag gtcttcgccc ctctcactgt
     1141 tctggaaaac ttgaagatgg gagctttccc tcggggaaat gacagtaaag tcgaagaaga
     1201 ctatgaatgg gtcttctccc tttttccccg tctgaaggag cggattagcc aatacgcggg
     1261 cacgctttcg ggaggagaac agcagatgct cgccatcggc agggcgctca tgtcccgtcc
     1321 gaaggtgctc ctccttgacg agccttcgct cggacttgcc cccattatca tcaaggatat
     1381 cttcaaagag ctgcgcagga ttaataacga aggagtcaca atcctgctgg ttgaacagaa
     1441 cgcccgccag gctcttctgc tctcaaatcg cggctatgtg attcagaccg ggcgtctggt
     1501 cacggaaggt ccctcggagg aacttcttgc gaatgcggac atcaaggcgg cctatctcgg
     1561 cgggggccac gcgaagggga agacgaccca agcgcttgcc tgatccttgc tctctcgctg
     1621 aatttacgag aaacacccgg tctctccgga agaccgggtg tttttttcga taataaactt
     1681 gacaaaaata gtaggatata gtactctgtc gtcagttcag agcaggggaa gtgatggcaa
     1741 ttaaactctc gacaaaaact cgctatggtc ttcgcgcctt gctctatatg gctgaaagat
     1801 acgacggtga acgaactgtg cccgtcggcg agatcgccaa ggagcaggaa gtttccgaaa
     1861 aataccttga gcagcttttt ctcaagctca gacgcagcaa ccttatacag agcgtccggg
     1921 gggcgcaggg cggctatatg ctcggcaggc cgcccgagga tattacgatc gcatgtgtgg
     1981 tggattctct tgaaggggat attacctttg cggactgtct ttcgccgggc ggatgcagaa
     2041 atcagggttc ctgcccgacg cacgaactct ggagccgttt gaaaggaagt atcgacgaga
     2101 tcctggagga taccacgctc gcggatcttc taaaaaattc agcgacgaac cacgccgggg
     2161 aaaaatccgg caagggaagt tgttcaagca aatagaaggg ggaaaaaaat aatgtccaga
     2221 caggtctatc ttgattacgc cgctactacg tttactgcgc cggaagtcgt ttccgcgatg
     2281 accccgtact ttacgcagtc gttcggcaat ccttcgtcga tctattcttt ttccgagagc
     2341 aaccgcaagg ctatgggcat agcaaggcag caggtggcgg atgtgctcgg cgcggcgaag
     2401 gaagaaattt ttttcacgag cggcggcact gaagccgata actgggctct caaaggggta
     2461 gcgtggcgaa atcgttcaaa ggggaaccat gttattacga cgaagatcga acatcatgcc
     2521 gtgctgcata cggcacagtt ccttcaggag aacggttttg aagtgacgta cctcgacgtg
     2581 gacgccgaag gcagggtgcg tcttgaggac ctcaaaaatg cggtgaccga gaagacaata
     2641 cttgtttcgg tgatgtttgc caacaacgaa atcggcacaa tccaacctat agccgagata
     2701 ggcgcattct gccgtgagaa gggaatcatc ttccacacgg acgcggtcca ggcggtggcg
     2761 catgtcccca tcgacgtgaa ggcgatgaat atcgacttgc tctccctttc tgcgcataaa
     2821 ttctacggtc ccaagggagt cggggcgctc tatgtccgca agggaataaa gattgacaac
     2881 ttcatgcacg gaggcggtca ggagcgcgga aagcgtgcga cgaccgagaa cgtggcgggt
     2941 atcgtaggac tcggcgcggc aatcgaactg actgcgtcca ggatgaagga cgcgtcttcc
     3001 cgcgtcggaa agctccgtaa catgctgatc gacggcgtga tgaaggttat ccctcatgcg
     3061 aagctcaacg gcgctccagt aa
//
LOCUS       DAOTIL010000261         3075 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminobacteriaceae bacterium isolate AMR_MDS_3026
            NODE_37620_length_3075_cov_2.879801, whole genome shotgun sequence.
ACCESSION   DAOTIL010000261 DAOTIL010000000
VERSION     DAOTIL010000261.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868050
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminobacteriaceae bacterium (wastewater metagenome)
  ORGANISM  Aminobacteriaceae bacterium
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae.
REFERENCE   1  (bases 1 to 3075)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3075)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:19:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,224
            CDSs (total)                      :: 3,198
            Genes (coding)                    :: 3,184
            CDSs (with protein)               :: 3,184
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3075
                     /organism="Aminobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_37620_length_3075_cov_2.879801"
                     /isolate="AMR_MDS_3026"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:3034609"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..247
                     /locus_tag="P5201_06410"
     CDS             <1..247
                     /locus_tag="P5201_06410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015557129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="alanine--glyoxylate aminotransferase family
                     protein"
                     /protein_id="HRV98202.1"
                     /translation="SVALYPKREALCSPTVTAALVPQGWTWNELNGALRSRGMAVGGS
                     YGPLAGKVFRIGHMGSQADMKLVEEGMDVLENVLKSC"
     gene            complement(421..1269)
                     /locus_tag="P5201_06415"
     CDS             complement(421..1269)
                     /locus_tag="P5201_06415"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRV98203.1"
                     /translation="MFRKSVFVVCVLGVFVSGVAIASAADLSGKWNCDDSGQYYLRQI
                     GNTLYWYGERSAANPAWSNIFQGNIQGERVNGNWVDVPKGQASSRGVLQLQIKDNGNV
                     LEATHKTGGFGGSRWVRVGYVPGTPDVPVVREDCVGFNPNTAEVKKFGARWKIVDGNH
                     AMFDFENAENEARMALRIIKHYRMNQSCFVGRPDPSFSYLLVGGHAPSGNFGGEDCVQ
                     FNPNTMEVKQIGGRWKIVDGSHWVFDFGNKEDEARLAFAIIKKYGFTRSCFVGRPNPS
                     FQYLRK"
     assembly_gap    1431..1530
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <1531..2459
                     /locus_tag="P5201_06420"
     CDS             <1531..2459
                     /locus_tag="P5201_06420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013048303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HRV98204.1"
                     /translation="ETLNVYTIWSERYATAVFDEFTLETGIAVNFLRFPSGEVLARLI
                     AEKENPQVDVFFGGIADAFVAGKKEEIFEQYVPGGAETIPAAFRDPEGYWTGVAMNPI
                     CFMFNRPFLEKNALEPPASWQDLLDPVYHNGLIMADARTSGTAVSRLFSLVLAMGENE
                     AFAYQKKLDENMQQYTKSGAGGALPIGRGQAAGGVFFLVDALEMQQTGYDIVISYPRE
                     GVVYGVEAMGLVKGAKNPELAKRFLDWAASPDMQRLYEREKINLIPTHPEVTLENPSL
                     DMKNVKLLELDIEWSGKERQRLVERWVNEIVR"
     gene            complement(2960..3067)
                     /locus_tag="P5201_06425"
     CDS             complement(2960..3067)
                     /locus_tag="P5201_06425"
                     /EC_number="4.2.1.8"
                     /inference="COORDINATES: protein motif:HMM:NF015727.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008927 - mannonate dehydratase activity
                     [Evidence IEA];
                     GO_process: GO:0006064 - glucuronate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannonate dehydratase"
                     /protein_id="HRV98205.1"
                     /translation="MIWGETGKPGYGLYDRALGAMYLAGLWEALQQRKK"
BASE COUNT          693 a          832 c          818 g          632 t
ORIGIN      
        1 gagcgtcgcg ctctacccga agcgcgaggc gctctgctcc cccaccgtca ccgcggcgct
       61 cgtcccgcag gggtggacat ggaacgaact gaacggcgcg ctcaggagca gaggaatggc
      121 ggtcggcggc agctacggac cgctcgccgg caaggtcttc cgcataggac acatggggag
      181 ccaagcggac atgaagctcg tggaagaagg catggatgtc cttgaaaacg tgctgaaaag
      241 ctgttgagat cgtaagcaga tagagcagaa tcgtcttgtt ctttttctca acggagcctt
      301 cctctttttt ttaagaggaa agctccgttg agaagcacaa tgttttctct gctgccgatc
      361 cttgagcaaa aacactgttc tcagtttcga atgcaggacg tcctcccggg aaatgtgtta
      421 ctacttcctc agatactgga aacttggatt cggccttccc acaaagcacg acctcgtgaa
      481 tccgtacttt ttgatgatgg cgaaagccag gcgggcttcg tcctccttgt ttccgaaatc
      541 gaacacccag tggctcccgt cgacgatctt ccagcgaccg ccgatctgct tcacttccat
      601 cgtattcggg ttgaactgaa cgcaatcctc accgccgaaa ttgccggacg gggcatgtcc
      661 tccgacgagc aggtagctga acgagggatc cggacgcccg acgaagcagg actggttcat
      721 cctgtaatgc ttgatgatcc gaagcgccat gcgtgcctcg ttctcggcat tttcaaagtc
      781 gaacatcgcg tggtttccgt ccacgatctt ccagcgcgcg ccgaatttct ttacctcggc
      841 ggtattgggg ttgaaaccga cgcaatcctc gcggacaaca ggcacatccg gcgtgccggg
      901 gacgtacccg acgcgcaccc agcgggatcc gccgaatcct ccggtcttat gtgtggcttc
      961 cagcacgttg ccgttatctt tgatctgtag ctgaaggaca ccccggctgg aggcctgccc
     1021 cttgggcacg tccacccagt tgccgttgac tcgctctccc tggatgtttc cctgaaagat
     1081 attcgaccag gcaggatttg cggcgcttcg ctccccgtac cagtagagcg tgttccctat
     1141 ctgccgaaga tagtactgcc cgctgtcgtc gcagttccac ttgccggaaa gatccgccgc
     1201 ggatgcaatc gcaacgcccg acacaaagac cccaagaacg cacacaacga aaacactctt
     1261 tctaaacatg tttctcctcc tctccgcaca acaggatacg taatctctcc tcacaacagg
     1321 gtttgataag gcaaagcaaa gcgaagtgaa cagaaagagc gacttcaaag cgatcactaa
     1381 aagtaattgc ctgtgcgcta tcaaaaacag accaagcaga atctcaagag nnnnnnnnnn
     1441 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1501 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn cggagacgct gaacgtctac acaatctggt
     1561 ccgagcgcta tgcgacggcg gtcttcgacg agtttaccct cgagaccggg atcgctgtca
     1621 actttttgcg ctttccttcc ggcgaggtat tggcgcgcct gatcgccgag aaggaaaatc
     1681 cgcaggtcga cgtctttttc ggcggcatcg ccgacgcctt cgtcgccggg aagaaggaag
     1741 aaatcttcga acagtacgtt cccggagggg cggaaaccat tccagccgct ttccgcgacc
     1801 ctgagggata ctggacgggc gtggcgatga atccgatctg cttcatgttc aaccgcccgt
     1861 tccttgagaa aaatgctctt gagccgcccg catcgtggca ggatctgctc gaccccgtct
     1921 atcacaacgg actgatcatg gcggacgcga ggacatcggg gacggcggtc tcccgcctct
     1981 tcagccttgt gcttgcgatg ggagagaacg aagccttcgc ctaccagaag aagctggacg
     2041 aaaacatgca gcagtacacg aagagcggcg cgggcggcgc gcttcctatc ggaagaggcc
     2101 aggcggcggg aggcgtcttc tttttggtgg acgcgcttga aatgcagcag accggctacg
     2161 acatcgtcat cagctatccg cgcgagggcg tcgtctacgg ggtggaagcc atgggtctgg
     2221 tgaagggcgc gaagaatccc gagctcgcga agcggtttct ggactgggct gcaagtcctg
     2281 acatgcagcg cctctacgag cgggagaaaa tcaacctgat tccgacgcac ccggaagtga
     2341 cgctggagaa tccgtcgctg gacatgaaga atgtgaagct cctggagctg gacatcgaat
     2401 ggtcgggaaa agagcggcaa cgcttggtcg aacgctgggt gaacgagatc gtccgctgaa
     2461 gacgaagtga aaaagggtat attaagagag cgcgctaaaa acccattttc acggcacgct
     2521 ctcttcgttt ttttggagag catccacgag aatcacccat acgcgccatc acaggagaaa
     2581 gaagagattt aacggaaatt ttggtgttat tatagcactt tcttataaag gtaaacgaac
     2641 ggtaaaaaat acttttctct cacacgccga acagcaaatt tgaggtaaac tttcaaactt
     2701 tacagtatcc gtgatatact cctatcaatt tccaaagggt agttccgagc gcttcgatat
     2761 ttcggggaag attttcggac aagaaaggac ggactcacac catgattgcc gggctggccc
     2821 tggccgccgg aggtatggta atattcatgt ttttcgatgg gatagagaaa cgacaggcga
     2881 taatcgtgta ctcgaaatcc gcgggactat gcgcaaggaa ccgctccctt gcgcatagtc
     2941 tacccgaaaa gtttccctat tatttcttcc tctgctgcag cgcctcccag aggccggcga
     3001 ggtacatcgc gccgagcgca cggtcgtaga gtccgtaccc cggcttgccg gtttcgcccc
     3061 agatcatgcg gccgt
//
LOCUS       DAOTIL010000262         3074 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminobacteriaceae bacterium isolate AMR_MDS_3026
            NODE_37626_length_3074_cov_4.190460, whole genome shotgun sequence.
ACCESSION   DAOTIL010000262 DAOTIL010000000
VERSION     DAOTIL010000262.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868050
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminobacteriaceae bacterium (wastewater metagenome)
  ORGANISM  Aminobacteriaceae bacterium
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae.
REFERENCE   1  (bases 1 to 3074)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3074)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:19:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,224
            CDSs (total)                      :: 3,198
            Genes (coding)                    :: 3,184
            CDSs (with protein)               :: 3,184
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3074
                     /organism="Aminobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_37626_length_3074_cov_4.190460"
                     /isolate="AMR_MDS_3026"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:3034609"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..721)
                     /gene="speE"
                     /locus_tag="P5201_06430"
     CDS             complement(<1..721)
                     /gene="speE"
                     /locus_tag="P5201_06430"
                     /EC_number="2.5.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013047750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyamine aminopropyltransferase"
                     /protein_id="HRV98206.1"
                     /translation="MEIRQKRTNELWFTEEQSVNMKLSLRVSDVLLNITSPYQDILLI
                     QTAEYGRMLVLDGAIQITERDEFCYSEMMAHVALCSHPSPKRVLIVGGGDGGVLREVL
                     RHKCVEKATLVDIDEEVINASKKFLPTINVALEDPRADVRPMDAIEFIASSDDEFDVA
                     IVDSTDPVEFAAGLFRSPFYRGIFKALKKDGMVVAQTESPFTDASVVKGAFKEMSAVF
                     PIVRMCWGAMPTYPSGMWTYTI"
     gene            complement(825..2000)
                     /locus_tag="P5201_06435"
     CDS             complement(825..2000)
                     /locus_tag="P5201_06435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013047751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III PLP-dependent enzyme"
                     /protein_id="HRV98207.1"
                     /translation="MENEFQFDLERYMSQANFNRIKDFAQDKETPFLIVDLKKVEEKY
                     DELLDSLPFAKIYYAVKANPLEQVLRMLVQKGSCFDIASIYELDHILALGASPDRISY
                     GNTIKKARNIAYAYSKGVRLFATDSESDLRKLAKNAPGARVFFRILTDGSGADWPLSR
                     KFGAHPDTIFKLILLAAKLGLEPYGISFHVGSQQRDIGQWDNAISACKYLFDCAEAEG
                     ITLKMLNLGGGFPATYISPTHETSVYAAEIIRFLKEDFGENLPEIILEPGRFMAGDAG
                     VLVTEVVLISKKSEANQYSWVYLDAGKFGGLIETLDESIKYPIYSEKKGPVQETILAG
                     PTCDSMDILYENEKYALPASLEEGDRLYFFTTGAYTQTYSSVCFNGFPPLKAYVLEK"
     gene            2194..2880
                     /locus_tag="P5201_06440"
     CDS             2194..2880
                     /locus_tag="P5201_06440"
                     /EC_number="2.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF015679.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA];
                     GO_function: GO:0071972 - peptidoglycan L,D-transpeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase"
                     /protein_id="HRV98208.1"
                     /translation="MFYGKRKVPEWAKQVAVLIVLLVLTVSAWQWYRILEHRTEQEFS
                     APVPAPSPAPTPVPAPSPEPRPDLEPGPLPTAEPPAVIQPPVAPHEARIDEAWLHVVK
                     GQYRMYFYRGETVERVYQIAIGANGGQKLRVGDHRTPVGSFSVQQIQDSRKWTYDFGD
                     GKGAIRGAYGPWFIRLKTPGWKGIGIHGTHDPDSIGRMITQGCVRLRNEDLEELKDKV
                     FIGMKVIISE"
     gene            2890..>3074
                     /locus_tag="P5201_06445"
     CDS             2890..>3074
                     /locus_tag="P5201_06445"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HRV98209.1"
                     /translation="MDFHLVDLPLSRPGFRRFVSAWLLRDTARDRTYIVDPGPPSTWP
                     LLRDAVHSKGNGRVDAV"
BASE COUNT          732 a          767 c          818 g          757 t
ORIGIN      
        1 ctatggtgta ggtccacata ccgcttggat aggtgggcat cgctccccag cacatccgca
       61 cgatggggaa aacagcgctc atttccttga aagccccctt gacaacggat gcgtcggtga
      121 atggtgattc tgtctgggcg actaccatac cgtccttttt cagcgcctta aaaatacccc
      181 tgtagaaagg ggaacggaac aacccggcgg caaactcgac cgggtcggta ctgtcaacga
      241 tggcgacgtc gaattcgtcg tcgcttgatg cgataaactc gattgcgtcc ataggacgca
      301 catcggcgcg gggatcctca agcgccacgt tgatcgtggg cagaaatttt tttgatgcgt
      361 tgatcacttc ctcgtcgata tcaacgagcg tcgctttttc gacgcatttg tggcggagca
      421 cttcgcggag cacaccgccg tctccgccgc cgacaataag cactcgtttc ggagatggat
      481 gggagcagag cgcaacgtgc gccatcatct ccgaatagca gaactcgtcg cgttcagtaa
      541 tctggattgc gccgtcaagc accagcatcc ttccgtactc agcggtttga atgagcaaga
      601 tatcctgata ggggctggtg atattcaaaa gaacatccga cacgcgaaga gagagtttca
      661 tgtttacact ctgttcctcg gtgaaccaaa gttcgtttgt ccgtttttga cgaatttcca
      721 cgggacacgc ctcctttcac gaagaacgcc gtcaatagaa catcgggggc gggtatcttc
      781 ccgtccccga tggacattat tatgtcagta taagagaact tcctttattt ttcaagaacg
      841 tacgctttaa gaggaggaaa gccgttgaaa cagacggagg agtatgtctg cgtatacgct
      901 ccggtggtga aaaagtagag gcggtcgccc tcttcaagag aggccggaag ggcgtacttt
      961 tcattttcgt aaagaatatc catgctgtcg catgtgggtc ccgcgagaat ggtctcctgg
     1021 acggggcctt tcttctccga gtaaatcggg tatttgatgg attcatcaag cgtctcaatc
     1081 aatccgccaa acttgccggc gtcgagatat acccaggaat actggttcgc ttccgatttc
     1141 ttcgatataa ggactacctc cgtgacaagg acgcctgcgt ctcctgccat aaatctcccc
     1201 ggttcgagga tgatttccgg aagattttct ccgaagtctt ccttgagaaa acggatgatt
     1261 tccgcagcat atacggaagt ctcatgcgtc ggcgagatgt atgtcgcagg gaatcccccg
     1321 ccgagattga gcatttttaa agtaattcct tcagcttcgg cgcaatcgaa gaggtatttg
     1381 caagcggaga ttgcgttgtc ccactgaccg atgtcccgct gctgagaacc aacatggaaa
     1441 gaaatgccgt acggttcgag cccgagtttt gcagccaaga ggataagctt gaaaatagtg
     1501 tcaggatgcg cgccgaattt tctggagaga ggccagtcgg cgccgcttcc atccgtcagg
     1561 atgcggaaaa aaactcgtgc tccgggggcg tttttcgcca actttcgcag gtcgctctca
     1621 gaatccgtgg cgaaaagacg gactcctttg gagtatgcgt acgcgatgtt tcgcgctttt
     1681 tttatggtat tgccgtaact gattctgtcc ggcgaagcgc cgagagcaag aatgtgatcc
     1741 agctcgtaga tggaagcgat atcaaagcag ctccccttct gcacaagcat acggagcacc
     1801 tgttcaaggg ggttcgcctt tacggcataa tagatcttcg caaatggcag cgaatcaaga
     1861 agttcatcgt acttttcctc gacctttttc aagtccacaa taagaaaagg cgtttctttg
     1921 tcttgtgcaa aatcctttat cctattgaaa ttagcttggg acatgtaacg ttcgaggtcg
     1981 aactgaaact cgttttccac gtctctacct cctccttagg ggtaatttaa aaacttatgc
     2041 agtgtacgac cttcgagtca gaaatgcaag ggggaaccga aggaaaacga gcaaaaacgg
     2101 taagtatttt cctctctttc ttctttccgt ttccatggta gaatcaaatt ctgttgaaaa
     2161 acttccgcac cgaacgatga ggaggctcct ttcatgtttt atggcaaaag aaaagtacct
     2221 gagtgggcga agcaggtagc tgttctgatc gtcctgttgg tacttactgt ttccgcgtgg
     2281 cagtggtacc ggatacttga gcatcgcaca gaacaggaat tctccgctcc cgtcccggcg
     2341 ccttctccgg cgccgacccc ggttcccgcc ccttcaccgg agcctaggcc tgaccttgaa
     2401 ccggggcctc tccccactgc cgaacctccg gcggtgattc agccgcctgt tgccccccat
     2461 gaggcgcgga ttgatgaggc gtggctccac gttgtcaaag ggcagtatcg aatgtacttc
     2521 tatagaggag agactgtcga acgcgtatac cagattgcca ttggagcgaa cggcggacag
     2581 aaactgagag tgggggatca tcgtacgcct gtcgggagtt tttctgtaca gcagatccag
     2641 gattcaagaa aatggacgta tgatttcggc gatggaaaag gagctattcg aggcgcgtac
     2701 gggccttggt tcattcgact gaaaacaccg ggttggaagg gaatcggcat acacggtacc
     2761 cacgatcctg attctatcgg gaggatgatc actcaagggt gtgttcgcct taggaacgag
     2821 gatctcgaag aattaaaaga taaggtcttc attggaatga aagttattat ttcggaatag
     2881 gagggcgtga tggattttca tctggttgac ctcccgctat cgagacccgg tttcaggcgg
     2941 ttcgtttcag cgtggcttct acgagatacc gcaagagaca ggacatacat tgtcgaccct
     3001 ggcccgcctt ccacgtggcc tctgcttcgt gatgcggtgc acagcaaagg aaacggacgg
     3061 gtcgacgcag tgtt
//
LOCUS       DAOTIL010000263         3062 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminobacteriaceae bacterium isolate AMR_MDS_3026
            NODE_37830_length_3062_cov_4.475890, whole genome shotgun sequence.
ACCESSION   DAOTIL010000263 DAOTIL010000000
VERSION     DAOTIL010000263.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868050
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminobacteriaceae bacterium (wastewater metagenome)
  ORGANISM  Aminobacteriaceae bacterium
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae.
REFERENCE   1  (bases 1 to 3062)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3062)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:19:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,224
            CDSs (total)                      :: 3,198
            Genes (coding)                    :: 3,184
            CDSs (with protein)               :: 3,184
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3062
                     /organism="Aminobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_37830_length_3062_cov_4.475890"
                     /isolate="AMR_MDS_3026"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:3034609"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..920
                     /locus_tag="P5201_06450"
     CDS             <1..920
                     /locus_tag="P5201_06450"
                     /inference="COORDINATES: protein motif:HMM:NF012711.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HRV98210.1"
                     /translation="FHTGGWNLLTLPLLHVGGRLVINRQFDPKKTLEIVEKERITTLF
                     GAATIFRMMVELPEFASADFSSVKWIMAGAAPTPVNIMEKFWNKGVKFVLGYGMTEAG
                     PNNISSCAHLISDDVMKAKYASVGKPMYLSMVKLADDDGNEISEPNTPGELLWGGPQT
                     FSGYWNNPDETAKTLKDGWVHSGDMAVRDEDGYYYIVGRKKNMFISGGENVFPPEIER
                     AMYDIAEIHEVCVFGVPDEKWGEVGKAVVSLKPGKRVSKEEIVAALGSKLARYKIPKY
                     IAFVDEVPKNNVGKIVVSKVVELYGRATD"
     gene            921..1958
                     /locus_tag="P5201_06455"
     CDS             921..1958
                     /locus_tag="P5201_06455"
                     /inference="COORDINATES: protein motif:HMM:NF005308.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-ACP synthase"
                     /protein_id="HRV98211.1"
                     /translation="MGVTLLKSADLVGIRSIGSYVPSGIRGSAYIAAASGIPEKVIRE
                     KFGINLVHKAPPEESVSAMGAKAALRALEGIDPASLDLVVYCGSEYKDYYLYNCAAKI
                     QHEIGAVNANAFEIHSLCSAGVYSLKVLKGMMLADDDLKRVLLVSSSKEGDLINYADG
                     ESRFMFNFGDGAAAAVLEKGLARNVVLETAMITDGSFADDIGVMGIGCKNFAGFSDMP
                     YGDRFLKVADIARMKERLDPVTLDNFVRVIERAAEKSGYAKDAIGFIAPIFMKRSILE
                     TMLASFGLNEENSFVLEDYGHCQSADAFIALQNGESLGRLKEGDVAVLVAAGTGYTWA
                     ATAVKWGAWSA"
     gene            2014..2430
                     /locus_tag="P5201_06460"
     CDS             2014..2430
                     /locus_tag="P5201_06460"
                     /inference="COORDINATES: protein motif:HMM:NF024844.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MaoC family dehydratase"
                     /protein_id="HRV98212.1"
                     /translation="MKGKTIKEIHAGDTASFQKTLSESDVYLFAGVSGDQNPAHINET
                     YASGTRFKTRIAHGMLVASLISAALGMELPGPGTIYLGQTIKFIAPAHIGDTVEARVT
                     VTEIDERRNIVTLETVCTNQDGKVLLEGVATVMPPK"
     gene            2444..>3062
                     /locus_tag="P5201_06465"
     CDS             2444..>3062
                     /locus_tag="P5201_06465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016293398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl synthase N-terminal-like
                     domain-containing protein"
                     /protein_id="HRV98213.1"
                     /translation="MSERIVLAGAVRTPIGKFGGTLAEVPVEKLGALVIKEALRRAGV
                     APGQVDEVLMGCILQACRGQNVARQAAIGAGLPVEVPATTLNNVCGSGLKAVNLAADM
                     IRAGSAEIVVAGGMENMSAAPYALEKARSGYRMGDGRCVDTMVLDALTDAFNNYHMGV
                     TAENVAGKYGITREMQDEFAAESQRKCEAAWNAGRFNGEIVPVPVT"
BASE COUNT          609 a          893 c         1079 g          481 t
ORIGIN      
        1 tcttccacac cggcgggtgg aacctgctga ccctgccgct gcttcacgtc ggcggacggc
       61 tggtcatcaa caggcagttc gatccgaaga agacgctcga gatcgtcgaa aaggaacgga
      121 tcaccacgct cttcggcgcg gccacgatct tcaggatgat ggtggagctg ccggagttcg
      181 cctccgcgga cttctccagc gtgaagtgga tcatggcggg ggccgccccg acgcccgtca
      241 acatcatgga gaagttctgg aacaagggcg tgaagttcgt ccttggatac ggcatgacgg
      301 aggccggccc gaacaacatc tcctcgtgcg cgcacctcat atccgacgac gtcatgaagg
      361 cgaagtacgc ctcggtgggc aagcccatgt acctttcgat ggtgaagctc gcggacgacg
      421 acgggaacga gatctccgag ccgaacaccc ccggcgagct gctgtggggc gggccgcaga
      481 ccttctccgg ctactggaac aacccggacg agacggcgaa gacgctgaag gacggctggg
      541 tgcattcggg ggacatggcg gtgcgcgacg aggatggcta ctactacatc gtcggccgga
      601 agaagaacat gttcatcagc ggcggcgaga acgtctttcc gccggagatc gagcgggcga
      661 tgtacgacat cgcggagatc cacgaggtct gcgtctttgg cgttcccgac gagaagtggg
      721 gcgaggtggg caaggccgtc gtctcgctga agccagggaa gcgcgtctcg aaagaggaga
      781 tcgtcgcggc gctcggctcg aagctcgccc gctacaagat accgaagtac atcgccttcg
      841 tggacgaggt gcccaagaac aacgtgggca agatcgtcgt ttcgaaggtg gtggagctct
      901 acggcagggc gacggactag atgggagtga cgcttttgaa atccgctgat ttagtcggca
      961 tacgcagcat agggagctac gtcccctccg gcatccgggg gagcgcgtac atcgcggcgg
     1021 cctcggggat tcccgagaag gtcatccggg aaaagttcgg catcaacctg gtgcacaagg
     1081 ctccgcccga ggagtccgtc tccgcgatgg gcgcgaaggc ggcgctgagg gcgctggagg
     1141 gaatcgaccc ggcgtcgctc gacctggtgg tctattgcgg cagcgagtac aaggactact
     1201 acctctacaa ctgcgcggca aagatccagc acgagatcgg ggcggtcaac gcgaacgcct
     1261 tcgaaattca cagcctctgt tcggcggggg tctattcgct caaggtactg aagggcatga
     1321 tgcttgcgga cgacgatctg aagcgcgtcc tgctggtctc ctcctccaag gagggggacc
     1381 tgatcaacta cgccgacggc gaatcgcgct tcatgttcaa cttcggcgac ggcgcggccg
     1441 cggcggtgct cgaaaagggg ttggcgcgga acgtcgtgct ggagaccgcc atgattaccg
     1501 acggaagctt cgcggacgac atcggcgtta tggggatcgg gtgcaagaac ttcgccggct
     1561 tcagcgacat gccgtacggc gaccgctttt tgaaggtcgc ggacatcgcc cggatgaagg
     1621 agcggctcga tccggtcacg ctggacaact tcgtccgggt gatcgaacgg gcggcggaga
     1681 agagcgggta cgcgaaggac gccatcggtt tcatcgcccc catcttcatg aagcgctcca
     1741 tcctcgagac catgctcgcc agcttcgggc tgaacgagga gaactcgttc gtgctggagg
     1801 actacggcca ctgtcaatcc gccgacgcgt tcatcgccct ccagaacgga gagtcgctgg
     1861 ggaggctgaa ggaaggggac gtcgccgtcc tcgtcgcggc gggcacgggg tacacatggg
     1921 ccgctacggc ggtgaagtgg ggcgcgtgga gcgcgtagcc ggaggaggca cggaggagaa
     1981 gccccccgtc cgggggcgcc gaaggaggac gctatgaagg gaaagacaat caaggagatc
     2041 catgcaggcg acacggcttc gttccagaag acgctgagcg agtcggacgt gtacctcttc
     2101 gccggggtga gcggagatca gaatccggcc catatcaacg aaacgtacgc ctccggcacg
     2161 cgattcaaga cccgcatcgc ccacggcatg ctcgtcgcct cgctgatctc cgccgctctc
     2221 gggatggagc tgcccgggcc ggggaccatc tacctcgggc agacgatcaa attcatagcc
     2281 cccgcccata tcggcgacac ggtggaggcg agggtgaccg tcacggagat cgacgagcgg
     2341 aggaacatcg tcacgctgga gaccgtctgc acgaatcagg acgggaaggt gctgctcgaa
     2401 ggcgtcgcga ccgtgatgcc gccgaagtga gggagggaat gcgatgagcg aacggatagt
     2461 gctggcgggc gcggtgcgca ccccgatagg gaagttcggc gggacgctcg ccgaggtccc
     2521 cgtcgaaaag ctcggcgcgc tggtgatcaa agaagcgctt cggcgcgcgg gcgttgcgcc
     2581 cggtcaggtg gacgaagtcc tgatggggtg catccttcag gcctgccgcg ggcagaacgt
     2641 ggcgcggcag gcggcgatcg gcgcggggct ccccgtggag gttcccgcga cgacgctgaa
     2701 caacgtctgc ggctccggcc tcaaggcggt gaacctggct gcggacatga tccgcgccgg
     2761 gagcgccgag atcgttgtgg cgggaggcat ggagaacatg tccgccgccc cctacgcgct
     2821 ggagaaggcg cggtccggct accgcatggg cgacggccgc tgcgtggaca ccatggtgct
     2881 cgacgcgctg acggacgcct tcaacaacta ccacatgggc gtgacggccg agaacgtggc
     2941 cgggaagtac ggaatcaccc gagagatgca ggatgagttc gccgccgaaa gccagcggaa
     3001 gtgcgaggcg gcgtggaacg ccggacgctt taacggggag atcgtccccg ttccggtgac
     3061 gg
//
LOCUS       DAOTIL010000264         3034 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminobacteriaceae bacterium isolate AMR_MDS_3026
            NODE_38282_length_3034_cov_3.598187, whole genome shotgun sequence.
ACCESSION   DAOTIL010000264 DAOTIL010000000
VERSION     DAOTIL010000264.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868050
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminobacteriaceae bacterium (wastewater metagenome)
  ORGANISM  Aminobacteriaceae bacterium
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae.
REFERENCE   1  (bases 1 to 3034)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3034)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:19:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,224
            CDSs (total)                      :: 3,198
            Genes (coding)                    :: 3,184
            CDSs (with protein)               :: 3,184
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3034
                     /organism="Aminobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_38282_length_3034_cov_3.598187"
                     /isolate="AMR_MDS_3026"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:3034609"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(145..972)
                     /locus_tag="P5201_06470"
     CDS             complement(145..972)
                     /locus_tag="P5201_06470"
                     /EC_number="2.7.11.33"
                     /EC_number="2.7.4.28"
                     /inference="COORDINATES: protein motif:HMM:NF003742.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016776 - phosphotransferase activity,
                     phosphate group as acceptor [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA];
                     GO_process: GO:0006470 - protein dephosphorylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate, water dikinase regulatory protein"
                     /protein_id="HRV98214.1"
                     /translation="MERVDTAPTCELFYLSDYLGEEMDSAASWITRRFEKIDIEISRY
                     GLLRTEGAVQKVLDEAARRDAFVLHGFLSGAFRRIVVERCAELGLDEYDVLRSLFSRL
                     SRIAGEEPRRDPSLVHPMDNEYFKRVKAIEYTIAADDGGNSSILKEADVVIIGVSRTG
                     KSPLCMYLAHKGIKAANIPLVPEIEPPVQLFQIEPARIIGLTRSSHSLEHIRTTRMHM
                     MGFDPDRSSYVKFERILSEIDYASGIMKRLGAVVFDVTARAIEETAHEILKLLRDGH"
     gene            complement(998..1804)
                     /locus_tag="P5201_06475"
     CDS             complement(998..1804)
                     /locus_tag="P5201_06475"
                     /EC_number="2.7.11.33"
                     /EC_number="2.7.4.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013048160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016776 - phosphotransferase activity,
                     phosphate group as acceptor [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA];
                     GO_process: GO:0006470 - protein dephosphorylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate, water dikinase regulatory protein"
                     /protein_id="HRV98215.1"
                     /translation="MESLNLFIVSDSTGETAEHVALAAISQFNTDFANIYRYRYVEGE
                     ARIDEILAKASEAPSMVICTLVDKKSRSALAAKCAQHDTPFVDLLGPVIDALEIRLER
                     APLQAPGLLRKLDEDYFQRIKAVEFAIQCDDGRSPESLPEADLVILGVSRTGKTPLSM
                     YIANKGFRAANIPLIPEVDPPEILKTIPSSRIVGLIISGERLSQIRQERLRLLGLEPK
                     ASAYANRERIERELQTAREYLQSLGARIYDVTDRAVEETAQEILDFLRSK"
     gene            complement(1840..>3034)
                     /gene="glyS"
                     /locus_tag="P5201_06480"
     CDS             complement(1840..>3034)
                     /gene="glyS"
                     /locus_tag="P5201_06480"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013048159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009345 - glycine-tRNA ligase complex
                     [Evidence IEA];
                     GO_function: GO:0004820 - glycine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006426 - glycyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit beta"
                     /protein_id="HRV98216.1"
                     /translation="KNGKLLPYFVGVSNNLASNMNVVREGNERVLRARLEDAAFFWAE
                     DLKKPLASNVERLKTIVYQEKLGTLHDKVMATRDLALWLCAETGMKDVLQLLDRAALL
                     AKADLVTNMVYEFPDLQGVMGREYALRNGEPERVARALYEQYLPKAAGSELPEDIIGA
                     LLGLAERVHIITSCFKVGFEPTGSQDPYALRRAARCINEILWGKAMDVNLGRLVARSC
                     EQLAIQEDVRERIVAFLQQRLHVQIREKGYGHELSTLAVSVAGSRPLQVLRFLEVFSK
                     LQAEQWFVDLVTSAVRVRNILAKSGDASAAVDESLFAKEAESKLYAEIVRINPLVEEA
                     LAGQDWKALAGYLSELSPFVTSFFEDVLVMDPDERVKCNRIHLLGLCNALFLKVGDLG
                     ILKGV"
BASE COUNT          617 a          867 c          842 g          708 t
ORIGIN      
        1 gcccgtacgt caccatctgg gcgaggttgg cccctttgcc cccgaggagc gccttcatgt
       61 ccgcgttgcc ttctttgaaa ctgtagatgt acttctcttt cgtcatctct atactgcacc
      121 tccgtaggtt tggataagat actttcagtg tccgtcgcga agcagcttca gtatttcgtg
      181 cgccgtctcc tctatagctc tggcagtgac gtcgaaaaca accgctccaa gacgtttcat
      241 tatacccgat gcgtagtcta tttcactcaa aatccgttcg aattttacgt aactgcttct
      301 gtccggatcg aaccccatca tgtgcatacg cgtcgtccgg atatgttcca gagaatggct
      361 cgaacgtgtc aggccgataa ttcttgccgg ctctatctga aagagctgaa ccgggggctc
      421 gatttcaggg acaagcggta tgttggcggc tttaattccc ttgtgggcaa ggtacatgca
      481 cagaggagac ttcccggttc tggaaacgcc gatgatgacg acgtcggctt ctttcaggat
      541 cgacgagttc ccgccgtcgt cggcggcaat agtgtactcg atcgccttta cccgcttgaa
      601 atactcattg tccatcgggt gtaccaggga cgggtcgcgc ctgggctctt ctccggcgat
      661 tcgcgagagg cgggagaaca gagaccggag cacatcgtat tcgtcaagtc cgagttcggc
      721 gcacctttcg acaacaatgc ggcgaaaagc gccggagagg aatccgtgaa gcacgaaggc
      781 gtctcttcgg gcagcttcgt cgagcacttt ctgcacggcg ccttcggtac gaagaagacc
      841 gtatcgggat atttcgatat cgatcttttc aaagcgcctc gtaatccacg acgcggccga
      901 atccatctcc tctcctaaat agtcggagag gtagaaaagt tcgcacgtcg gcgcggtatc
      961 gacacgttcc atactgccgg aaaggtcttc tctcaatcta tttcgaacgc aggaaatcga
     1021 ggatctcctg cgcggtctct tcgacggccc tgtcggtaac gtcgtaaatg cgggcgccga
     1081 gcgactggag atactccctg gcagtttgaa gttctctttc aatccgctcc cggtttgcat
     1141 aggccgacgc cttcggttcc aatcccagaa ggcgaagacg ctcctgacgg atctgtgaga
     1201 gcctttctcc ggagatgatc agccctacaa tcctcgatga cgggatggtt ttaagaatct
     1261 caggcggatc gacttccggt atgaggggga tattcgccgc cctgaatcct ttgttcgcga
     1321 tgtacatcga gagcggcgtc ttcccggtac gggagactcc aaggatgacc aggtccgcct
     1381 ccggcagcga ttcggggctg cgtccgtcgt cgcattggat ggcgaattcc acggccttga
     1441 tccgctgaaa atagtcctcg tcgagctttc tgagaagtcc cggcgcctgg agcggagccc
     1501 tttcgaggcg aatttcgagc gcatcgatga caggccccag cagatcaacg aaaggggtgt
     1561 cgtgttgggc gcacttggcg gcgagcgcgg agcggctctt tttgtccacc agcgtacaga
     1621 tcaccatgga gggagcttcc gacgcctttg cgagtatttc gtcgattctg gcctcccctt
     1681 cgacataccg gtaacggtaa atgttggcaa aatctgtatt gaattgactg atggccgcca
     1741 gagcgacgtg ctccgccgtc tcgccagtgg aatcggatac gatgaacaaa ttcaaggatt
     1801 ccattgcttc acactcctta tgttgacgcc gctctcaggc tacacgccct tgagtattcc
     1861 caggtccccg accttcagga agagggcgtt gcacagacct agaagatgta tcctgttgca
     1921 ttttaccctt tcatcggggt ccattacaag gacgtcttcg aaaaatgacg taacaaatgg
     1981 cgaaagttcc gacagatacc cggcaagggc cttccaatcc tgcccggcga gcgcttcttc
     2041 gacgagggga ttgattctga cgatctcggc gtagagtttg ctttccgcct ccttggcgaa
     2101 aaggctttca tcgacggctg cggaggcgtc gccgctcttg gcaaggatat tgcgaactct
     2161 gacagccgat gtgacgagat cgacaaacca ctgctccgcc tgaagcttgg agaagacttc
     2221 gaggaagcgc agcacctgaa gcggacggct tcccgccact gaaacggcga gcgtggaaag
     2281 ctcgtggccg taccccttct cgcgaatctg cacgtggagc cgctgctgta ggaaggcgac
     2341 gatcctttcg cggacatcct cctggattgc taactgctcg cagcttctgg ccaccagccg
     2401 cccgagattg acatccattg cctttcccca gaggatctca ttgatgcagc gggccgccct
     2461 tcgaagggcg tacggatcct gagatcctgt aggttcgaac ccgaccttga agcagcttgt
     2521 gataatgtgc acccgttcgg ccagtccgag aagggcgccg atgatatctt caggcagctc
     2581 gctccccgcg gccttgggaa gatactgttc gtagagcgcc ctggccaccc gttcaggctc
     2641 tccgttgcgg agggcgtact ctcttcccat gactccctgg aggtcgggga actcatagac
     2701 catgtttgtc acaaggtcgg cctttgcaag aagagcggcc cggtccagaa gctggagcac
     2761 gtctttcatc cccgtctccg cgcagagcca gagcgcaaga tcgcgggtcg ccatcacctt
     2821 gtcgtggaga gtaccgagtt tttcctggta gacgattgtt ttcagccgct cgacgttgga
     2881 tgcgaggggc tttttcagat cctccgccca aaagaacgcc gcgtcctcga ggcgtgcgcg
     2941 aaggacccgc tcgtttccct cgcggacgac gttcatattc gatgcgagat tgttgcttac
     3001 cccgacgaaa taaggaagga gtttcccgtt cttt
//
LOCUS       DAOTIL010000265         3018 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminobacteriaceae bacterium isolate AMR_MDS_3026
            NODE_38577_length_3018_cov_2.174485, whole genome shotgun sequence.
ACCESSION   DAOTIL010000265 DAOTIL010000000
VERSION     DAOTIL010000265.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868050
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminobacteriaceae bacterium (wastewater metagenome)
  ORGANISM  Aminobacteriaceae bacterium
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae.
REFERENCE   1  (bases 1 to 3018)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3018)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:19:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,224
            CDSs (total)                      :: 3,198
            Genes (coding)                    :: 3,184
            CDSs (with protein)               :: 3,184
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3018
                     /organism="Aminobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_38577_length_3018_cov_2.174485"
                     /isolate="AMR_MDS_3026"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:3034609"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..308
                     /locus_tag="P5201_06485"
     CDS             <1..308
                     /locus_tag="P5201_06485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006300176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HRV98217.1"
                     /translation="PTSKILFSGDIGAAIFPRGTRYPVAEDFDAHLKYMEGFHKRYMA
                     SNAFCRRWVKMVSSLDVEHIAPQHGALIKGRENVKKFLAWFENLACGVDVLDDIYGR"
     gene            326..1168
                     /locus_tag="P5201_06490"
     CDS             326..1168
                     /locus_tag="P5201_06490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006300174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HRV98218.1"
                     /translation="MEHKFSQVIHQLGGAYFANTLMNSFMAQLDEVLVSRVRAVEEEI
                     DATSERFVQLSALLGDLLKEFEKSGKETRESVGRITQMNEDLGEELKRSGTDLDGMSE
                     NVSRTVDATFGTLESFLEIEKMSKEIQRIAKQTNLLALNASIEAARAGEHGRGFAIVA
                     KNVQELAAETKAASESIGSKVGEISGAVQNAMEDIRRVSEMFDVIRRSLSTFTEFLST
                     NKSFMERMEHTMADAGNKVRRSSEEMDNSVEVMGNASKKFGSMAATISAIVLAQKNLK
                     QIRL"
     gene            1205..2377
                     /locus_tag="P5201_06495"
     CDS             1205..2377
                     /locus_tag="P5201_06495"
                     /EC_number="3.1.4.-"
                     /inference="COORDINATES: protein motif:HMM:NF014069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HD-GYP domain-containing protein"
                     /protein_id="HRV98219.1"
                     /translation="MRVLKDVDVDELHLFRGRVVEDVVSSTGSLLLPKGSEIQSVLES
                     VPGITKTLKRWGVYKVMLDDSFNISEEDFNKIIENIEPKIRVLEAALAQNTITQVDEV
                     YRRISENGSLGEGAYMLARQAALLTEEVTRAPQILLCLGRVRDSDEYTFVHSLNVGLL
                     CGYLAGRLKPGDREFAASMTYGGLLHDLGKAKVPQQVLNKPGKLTEKEYEMMKAHSIY
                     GDEIARSSGISDVRVLSVIRNHHERWGGHGYPDGLQKNSIPLFARIAAVADVFDALTA
                     KRVYKNPLSSREAVSMILESSENDFDKGVVRELLLSVGLYPAGTLVELSDFSVGVVVG
                     ARNTDLFRPQVSVTIDGKGRRAPEGTIVDLGLQQDLFVRRALDDVGKGVAYSEKAG"
     gene            complement(2397..>3018)
                     /locus_tag="P5201_06500"
     CDS             complement(2397..>3018)
                     /locus_tag="P5201_06500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013047446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HRV98220.1"
                     /translation="AGRKGLFDPGYVTWLAKSPFERRGFSTKEIFNELLEAAPEKANV
                     SLRPSFGRLLRKEVLPEVEAEFIARYSLPQQDEYLVGNSIRSGMKRIDRAMSKMVHHH
                     ERKKFRKTLASLWYDEMDIEENLEMAFLFHTEQSPSALLAAQVILPFERNYLQALLKV
                     KRFANRMPKGFAFRSMKELNNTVDEIDPTIYGFEEKIGEIERSLFS"
BASE COUNT          645 a          837 c          939 g          597 t
ORIGIN      
        1 acccgacgtc gaagattctc ttctcgggcg atatcggcgc ggcgatcttc ccgcgcggaa
       61 cccgctaccc cgtagccgag gattttgacg cgcacctgaa gtacatggag gggttccaca
      121 aacgctatat ggcctcgaac gctttctgcc gcagatgggt gaagatggtc tcttcgctcg
      181 atgtggagca catcgccccg cagcacggcg ccctgatcaa ggggcgggag aacgtgaaga
      241 agtttctcgc gtggtttgaa aacctcgcct gcggcgtgga tgtcctcgac gacatctacg
      301 gtcgctagcc ggggagggtc gggggatgga gcataaattt tcacaggtaa tccatcagct
      361 tggaggggcg tacttcgcca acacgcttat gaactccttc atggcgcagc tcgacgaagt
      421 gctggtgagc cgcgtcaggg cagtcgagga ggagatcgac gcgacctcgg agcgcttcgt
      481 ccagttgagc gccctgctcg gggatctgtt gaaagagttc gaaaagagcg ggaaggagac
      541 cagagagagc gtcggacgca tcacccagat gaacgaggac ctgggggagg agctgaaacg
      601 ctccggcacc gacctcgacg gcatgagcga gaacgtctcc cggacggtgg acgccacgtt
      661 cggcactctg gagtccttcc tggaaataga gaagatgtcc aaggaaatac agcgcatcgc
      721 caagcagacg aatctgttgg cgctcaacgc ctccatagag gccgcacggg caggcgagca
      781 cgggcggggg ttcgccatcg tggcgaaaaa cgtccaggag ctcgccgcgg agaccaaggc
      841 ggcttccgaa tccatcggct ccaaggtggg cgagatctcc ggcgcggtgc agaacgccat
      901 ggaggatatc cggcgggtga gcgagatgtt cgacgtcatc cggcgttcgc tttcgacgtt
      961 caccgagttt ctgtcgacca acaaatcgtt catggagcgt atggagcata ccatggccga
     1021 cgcggggaac aaggtccgcc gcagttcaga ggagatggac aactccgtcg aggtgatggg
     1081 gaacgcgtcg aagaagttcg gttcgatggc ggcgaccata tccgcgatcg tccttgccca
     1141 gaagaacctg aagcagatac gactttaagc cggaggtttc ggcgggaggg aaggcggggt
     1201 tgccgtgcgg gtgctgaaag atgttgatgt cgacgaatta cacctgttcc gcggacgggt
     1261 cgtcgaagat gtggtgtcgt cgaccggttc gctcctttta ccgaagggga gcgagattca
     1321 gtcggttctg gagtccgttc ccgggatcac aaagacgctc aagcgctggg gcgtctataa
     1381 ggttatgctc gacgactcct tcaacatttc ggaggaggac ttcaacaaga taatcgagaa
     1441 tatcgagccg aagatccgtg tattggaggc cgcgctcgca cagaatacaa ttacgcaggt
     1501 cgacgaagtc tatcggcgga tctccgagaa cggttccctt ggggaaggcg cctacatgct
     1561 ggcgaggcag gccgcgttgc tgaccgaaga ggttacgcgc gctccgcaga ttttgctctg
     1621 tttggggcgg gtgcgcgaca gcgacgagta cacattcgtc cactcactca acgtggggct
     1681 gctctgcggt tatcttgccg gacgtctgaa acccggcgac cgcgagttcg ccgcctcgat
     1741 gacgtacggc ggactccttc acgacctcgg taaagccaag gttccgcagc aggtgctcaa
     1801 caagccggga aaactgacgg agaaggagta cgagatgatg aaggcgcact ccatctacgg
     1861 agatgaaatc gccaggtcat ctggtatcag cgacgtccgc gtgctttctg tcatccggaa
     1921 tcaccatgag cgttggggag ggcatgggta ccccgacggt ctgcaaaaga acagtatccc
     1981 gctgtttgcc cgcatcgcgg cggttgccga tgtcttcgac gcgctcacag caaagagagt
     2041 gtacaaaaat ccgctgtcga gccgcgaagc ggtatcgatg attctggaat cctccgaaaa
     2101 cgacttcgac aagggcgtcg tccgggagtt gctgctctcc gtgggactct atccggccgg
     2161 cacgctggtc gagctttcgg atttcagcgt gggagtggtg gtcggcgcca gaaatacgga
     2221 tctcttccgc ccccaggtga gtgtcaccat cgacgggaag gggcgccgcg ctccggaagg
     2281 aaccatcgtc gacctgggat tgcagcagga cctctttgtc cgccgggcgc tcgacgacgt
     2341 agggaagggc gttgcgtaca gcgaaaaagc agggtaaaga cgcttgcact taaaattcag
     2401 gagaagagcg atcgttcgat ctctcctatc ttctcctcga acccgtagat cgtcggatcg
     2461 atttcgtcaa ccgtattgtt taactctttc atcgaccgga aggcaaaccc tttcggcatt
     2521 ctgttcgcga atcgtttcac cttgagcagc gcctggaggt agttcctttc aaagggaagg
     2581 atcacctgcg cggccagcaa agcgctgggc gactgctccg tgtgaaaaag aaacgccatc
     2641 tccaggttct cctcaatgtc catctcatcg taccagagcg aggcaagcgt ctttcgaaac
     2701 ttcttccgct cgtggtggtg gaccatcttc gacattgccc tgtcgatacg cttcattccg
     2761 gagcggatcg agttcccgac gagatactcg tcctgctgcg gcagggagta gcgtgcgatg
     2821 aactctgctt ccacttcagg gaggacctct ttgcggagca gcctgccgaa agacgggcgc
     2881 agcgagacgt tcgccttctc cggggccgct tcaagcagct cattgaagat ctccttcgtt
     2941 gaaaaccccc gccgctcgaa cggcgatttt gcaagccacg tgacataccc cgggtcgaag
     3001 agccccttcc gtcctgcc
//
LOCUS       DAOTIL010000266         3016 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminobacteriaceae bacterium isolate AMR_MDS_3026
            NODE_38602_length_3016_cov_3.347180, whole genome shotgun sequence.
ACCESSION   DAOTIL010000266 DAOTIL010000000
VERSION     DAOTIL010000266.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868050
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminobacteriaceae bacterium (wastewater metagenome)
  ORGANISM  Aminobacteriaceae bacterium
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae.
REFERENCE   1  (bases 1 to 3016)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3016)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:19:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,224
            CDSs (total)                      :: 3,198
            Genes (coding)                    :: 3,184
            CDSs (with protein)               :: 3,184
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3016
                     /organism="Aminobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_38602_length_3016_cov_3.347180"
                     /isolate="AMR_MDS_3026"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:3034609"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..586
                     /locus_tag="P5201_06505"
     CDS             <1..586
                     /locus_tag="P5201_06505"
                     /inference="COORDINATES: protein motif:HMM:NF015948.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF362 domain-containing protein"
                     /protein_id="HRV98221.1"
                     /translation="YAFDHTCDLWRNCYANSGIEAAVKRAGGIVSPAESASSYTKVSI
                     PGARVLKETSIHRLLLDCDVFINVPVLKHHGGAGITMGMKNLMGTVWNRGEFHSKGLH
                     QCIADVSLLRRPDLTVIDAYRMLTRNGPRGTSASDVTVLKAQILSTDIVAGDCAGARI
                     FGLDPKNVGHIRMGAELGAGTMDLDSLGVARIVL"
     gene            655..2088
                     /locus_tag="P5201_06510"
     CDS             655..2088
                     /locus_tag="P5201_06510"
                     /inference="COORDINATES: protein motif:HMM:NF024581.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HRV98222.1"
                     /translation="MTLFFLFFAGVREGGLPWLQAVPSVLRLWTGALILAASAALAGR
                     VYCSVLCPLGTLQELIWRTGKRSASYRKPSQLRYWVLGGVVVAAAAGYMFPAAFLDPY
                     AAFGRGVTHLLRPLAIGGNNMIATFLEERGVYGLLYRAGRSPLLPGVLWGTALFFALL
                     GFWSFFRGRPFCDALCPVGTFLGLFSVSPVLGVRFDGERCVACGKCESVCPEGCISVV
                     NRRVETDRCILCLRCLDACPAGGLKLGRLSPREGLVRRGALRFGLGALAALGAALRAR
                     LPDRTVSASASSWEERLPISPPGSGSHGNFTKKCVACTACVTACPAGIIRPSLSAWGM
                     QGIFQPELDYSKGYCQFECAACSSACPTGAIAPLTLDEKKRTSIGRARFFRRNCIVRK
                     YGQACGACSEHCPTQAVRMVPFRRNLTIPEVDPTLCLGCGACEYACPAEPKAITVSGV
                     SVHGKIRDRKNEGAEAPVVAPLEEFPF"
     gene            2192..2383
                     /gene="tatA"
                     /locus_tag="P5201_06515"
     CDS             2192..2383
                     /gene="tatA"
                     /locus_tag="P5201_06515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014461314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocase TatA/TatE family
                     subunit"
                     /protein_id="HRV98223.1"
                     /translation="MNLGMGEILVLLALALVLFGAKRLPEVGRAAGSAIREFKRGLES
                     GEAPEKAGEDTGETNEDGK"
     gene            2358..>3016
                     /gene="tatC"
                     /locus_tag="P5201_06520"
     CDS             2358..>3016
                     /gene="tatC"
                     /locus_tag="P5201_06520"
                     /inference="COORDINATES: protein motif:HMM:TIGR00945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocase subunit TatC"
                     /protein_id="HRV98224.1"
                     /translation="MKRTKTGSEGEFSDHLEVLRRKIIAVLFFFCCATALLFLLSERW
                     VRFLQAPLEGLGVSLYYFKPYEKFLTYMRLSFWGGAALSVPLAVLQAALFVAPALRKN
                     EMKYLILAGGLIPALFLAGAAFAYRFAAPLALRFFLFFGEGDNVLPLWGFGDYASFLF
                     SLLLASGMLFQAPLLLLLFILFGLVSVETLSRLRPWIILGIALIAALLTPPDVVSQIL
                     LG"
BASE COUNT          478 a          868 c          990 g          680 t
ORIGIN      
        1 gtatgctttt gatcatacgt gcgacctgtg gcggaactgt tacgcgaaca gcggcatcga
       61 agcggcggtg aaacgggccg gtggcatcgt ctccccggcg gagtccgcga gctcttacac
      121 gaaggtctcc attcccggcg cgcgggtcct caaggagacc tcgattcacc gtctgcttct
      181 cgactgcgac gtttttatca acgttcccgt gcttaaacat catgggggcg ccgggatcac
      241 gatggggatg aaaaacctta tgggaacggt atggaacagg ggagagtttc actcgaaggg
      301 gctgcatcag tgcatagccg atgtgagcct tctccgcagg ccggacctta cggtgatcga
      361 cgcgtacagg atgctcacgc ggaacgggcc gcgcgggacc tccgcgagcg acgtgaccgt
      421 actaaaggcg cagatcctct cgacggacat cgtggccgga gactgcgccg gggcgaggat
      481 tttcggcctt gatccgaaaa atgtcgggca tattcgtatg ggcgcggagc tcggcgcggg
      541 gacgatggat ctcgattcac tcggcgtagc gcgcatcgta ttgtgatgga caggaagaga
      601 gcgggagcgg cgcggaaact ccgctccctt tttgctctgg cggttcttct tctcatgacg
      661 ctgttctttc tcttcttcgc gggagtgaga gagggaggcc ttccgtggtt gcaggccgtt
      721 ccgtcggtac ttcgattgtg gaccggagcc cttatccttg ctgcaagcgc cgccctggcg
      781 gggcgcgtgt actgctccgt cctctgtccg ttgggaacgc ttcaggagct gatttggaga
      841 acgggaaagc ggagtgcgtc ctacagaaaa ccatcgcagc tccgctactg ggtgcttgga
      901 ggagttgtgg ttgcggctgc ggcggggtac atgttcccgg cggcgttcct cgacccctat
      961 gccgcgttcg gcaggggagt cacgcatttg ctccgacctc ttgcaatcgg tggaaacaat
     1021 atgatcgcca cgttcctcga agagcggggc gtctacggcc tgctttacag agccggccga
     1081 agcccgctgc ttcccggggt gctgtggggg acggcgctct tcttcgcgct tcttgggttc
     1141 tggtcgtttt tcagggggag accgttctgc gatgcgctct gccccgtcgg gaccttcctg
     1201 ggtttgtttt cggtctcgcc tgtcctcggc gtacgttttg acggtgaacg gtgcgttgcg
     1261 tgcggaaagt gcgagtccgt ctgtccagaa gggtgcattt cggtagtaaa ccgccgtgtg
     1321 gagacagacc gctgtatact ctgtcttcgc tgtcttgatg cgtgccctgc tggagggctg
     1381 aagctggggc ggctctcccc gcgcgagggg cttgtccgga gaggggcgct ccgtttcggt
     1441 ctcggcgcgc tcgcagcgct cggcgcggcc ttacgcgcca ggctgcccga cagaactgtt
     1501 tccgcgtcgg cgtcgtcctg ggaagagcgc ctgccgatat cgccgcccgg ctcggggagc
     1561 cacggcaact tcacgaaaaa gtgtgtggcg tgcactgcgt gcgttacggc ctgtcctgcg
     1621 ggaatcatcc gtccttcact ctccgcatgg gggatgcaag gcatttttca gccggaattg
     1681 gactattcaa aggggtattg tcagtttgaa tgcgcggcct gctcttctgc atgcccaacg
     1741 ggcgcgattg ccccgctgac gcttgatgaa aagaaaagaa cgtctatcgg aagagcgcgt
     1801 tttttccggc gcaactgcat cgtcaggaag tacggccagg cgtgcggcgc gtgctccgag
     1861 cactgcccga cgcaggctgt ccggatggtt ccgttccgcc gcaacctgac cattcccgag
     1921 gtggatccga cgctgtgtct tggctgcggc gcgtgcgaat acgcctgccc ggccgagccg
     1981 aaggcgatca ccgtttccgg cgtctcggtc cacgggaaga tccgggaccg gaagaacgag
     2041 ggggcggaag ctcctgtggt ggccccgctg gaggagttcc cgttctgaac cttagggaaa
     2101 gtacgagcga tcacgcgcgg gagcgctccg ccggaaacgc tgagccgcgc tcttgcgccg
     2161 agaaaggacg cagagaagga ggatggttgg tatgaacctg ggaatgggag aaattctggt
     2221 acttctcgcg cttgccctgg tgctcttcgg agcgaagcgc cttcccgagg tcgggagggc
     2281 ggcgggaagc gcgatcaggg agttcaagag agggcttgag tcgggagaag cgccggaaaa
     2341 ggcgggagaa gatacgggtg aaacgaacga agacgggaag tgagggggag ttttccgatc
     2401 accttgaagt cctccggcgc aagattatcg ccgtcctctt tttcttctgt tgcgcgacgg
     2461 cgctgctctt tcttctctcc gaacggtggg tgcgcttcct ccaggctccg ctggaggggc
     2521 tcggagtatc tctctactat ttcaagccct acgagaagtt tctgacgtac atgcgtcttt
     2581 ccttctgggg cggcgcggcc ctgagcgtgc ctcttgccgt ccttcaggcc gcgctcttcg
     2641 tcgcgcccgc gctccgcaag aacgagatga agtacctcat tcttgcaggc ggtcttatcc
     2701 cggcgctctt tctcgcaggc gccgcgttcg cgtaccggtt tgcggcgccg ctcgccctcc
     2761 gtttcttcct cttcttcggc gagggagaca acgtgcttcc cctctgggga ttcggcgact
     2821 acgcgtcctt tctgttctcg ctgctgctgg cgtccggcat gctctttcag gcgccgctcc
     2881 ttctgttgct ctttattctg tttggtcttg tctccgtgga gacgctctcc aggctacggc
     2941 cgtggatcat tttaggaatt gcgctcatag cggcgctgct tactccgccc gacgttgtgt
     3001 cgcagatact cctcgg
//
LOCUS       DAOTIL010000267         3004 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminobacteriaceae bacterium isolate AMR_MDS_3026
            NODE_38802_length_3004_cov_3.548321, whole genome shotgun sequence.
ACCESSION   DAOTIL010000267 DAOTIL010000000
VERSION     DAOTIL010000267.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868050
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminobacteriaceae bacterium (wastewater metagenome)
  ORGANISM  Aminobacteriaceae bacterium
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae.
REFERENCE   1  (bases 1 to 3004)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3004)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:19:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,224
            CDSs (total)                      :: 3,198
            Genes (coding)                    :: 3,184
            CDSs (with protein)               :: 3,184
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3004
                     /organism="Aminobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_38802_length_3004_cov_3.548321"
                     /isolate="AMR_MDS_3026"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:3034609"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..664)
                     /locus_tag="P5201_06525"
     CDS             complement(<1..664)
                     /locus_tag="P5201_06525"
                     /inference="COORDINATES: protein motif:HMM:NF012706.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HRV98225.1"
                     /translation="MKKFRLIALALLAVAALALPAAAAELRIAQVADISSLDPHKVSD
                     IYSANVIRQIYSNLVQVDEHMEIVPDLAESWENPDNLTWIFHLRKGVTFHNGDPLTAA
                     AVKFSLERIKDPATASPGATHLREVASVEAIDEHTLKIVTKRPFAPMLMSLTRYETSI
                     VSERAVKEAGADYGSKAAVGTGPFKFMSWSRGDKVTLEKFDGYFRGPASLDTLVYRGI
                     PED"
     gene            complement(771..1874)
                     /locus_tag="P5201_06530"
     CDS             complement(771..1874)
                     /locus_tag="P5201_06530"
                     /inference="COORDINATES: protein motif:HMM:NF012766.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Xaa-Pro peptidase family protein"
                     /protein_id="HRV98226.1"
                     /translation="MNSMFAARMERLAEELRRREADALFVFSSELDNRPAVQYLSGFT
                     GSFAVVVAGVNGGRLVTDSRYFIQAEEESFLPLVRMEDRDPWPAVREALRQLGVRRLA
                     VEDDKLSLERGRALENLLPSLIGTTRLVQQLRAVKDAEELELLRRSARIAAEAFEAFL
                     PAIRPGRTEAELAADLVHEIRRRGAQQMAKGHFVVASGPRGARPHGIFSDRVVQPGDF
                     ITMDFGAVYNGYVSDMTRTVGVGDVDPKLVEIYHIVREAHDRAIAAVSPDATGSDVDE
                     AARSLIRGRGYGEFFSHSTGHGIGLELHELPVVNRMNGKKLPVGSVVTVEPGIYIDGL
                     GGVRIEDDVIVTPHGAEVITSASGTELRLLSPA"
     gene            complement(2089..>3004)
                     /locus_tag="P5201_06535"
     CDS             complement(2089..>3004)
                     /locus_tag="P5201_06535"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRV98227.1"
                     /translation="AEEDPGPLALMLGEGLLAKEGELFSLTGAGREAFSEEAAANYLP
                     AVPGIPGADKEKDLFRTRLRLLLDKKHIQRWGVKEFVLSARFPTPPLQDDELFTRSSV
                     FEWIWPSSPIIGQMREDWPVTGLAARKETPPPPDAAAKWFEAKGSYPAYFEADLLHLS
                     RYDFQAYAHIPRSPNDPWGILNADRFFCLQAQAPQAGNLDGFLATIGRFQLALEVLRR
                     MVLPGYIDLDSFDQDGINWLLFLFEREEEAEACVSLLAPFGLDLIRGSMPMDVWALSF
                     EALENFKSKAETIHDLIPVIGRPVVRTP"
BASE COUNT          501 a          935 c          996 g          572 t
ORIGIN      
        1 catcctcggg aatacctctg tagaccagcg tatcgaggga cgcggggccg cggaagtagc
       61 cgtcgaactt ttcgagcgtg accttgtcgc cgcgagacca gctcatgaac ttaaagggac
      121 cggtgcccac ggcggctttg ctgccgtagt ccgcgcccgc ttccttgacg gcgcgttcgc
      181 ttacgatgga ggtctcgtag cgggtcaggc tcatcagcat gggggcgaac ggccgcttcg
      241 tgacgatctt cagcgtgtgc tcgtcgatcg cctcgacgga ggccacttcg cgcagatggg
      301 tggcgccggg ggatgcggtc gccgggtcct tgatccgttc gagggagaac ttcactgcgg
      361 cggcggtgag cggatcgccg ttatggaagg tcacgccctt gcggaggtgg aagatccacg
      421 tgaggttgtc ggggttctcc cagctctcgg cgaggtcggg gacgatctcc atgtgttcgt
      481 cgacctgcac gagattgctg tagatctgcc ggatcacgtt cgcggagtag atatcgctga
      541 ccttgtgcgg gtcgaggctg gagatgtcgg caacctgggc tatccgcagc tcggcagcgg
      601 cggcgggaag agcgagcgct gcgacggcga gaagcgcgag tgcgatgaga cggaactttt
      661 tcactgtgca tcacgacctt tctgtttttt tcgacgccgc tttttccggc gtcttcgagt
      721 gggtgcgcaa tctatctatg cttcaccggg gagaaccccg gtactttttt ctacgccggg
      781 gagagcagac gcagctccgt cccggaggcg cttgtgatca cctccgcgcc gtgcggcgtg
      841 acgatcacgt cgtcctcgat gcgcacgccg cccaggccat cgatgtagat gcccggttcg
      901 acggtcacca cggagccgac gggaagcttt ttgccgttca tgcggttgac cactggcagc
      961 tcgtgcagct ccagcccgat gccgtgcccc gtcgaatgcg aaaagaactc gccgtacccg
     1021 cggccgcgga tgaggctccg cgccgcctcg tcgacgtcgc ttcccgtcgc gtcgggagaa
     1081 accgcggcga tcgcgcggtc gtgcgcctcg cggacgatgt ggtagatctc gacgagcttc
     1141 gggtcgacgt cgccgacgcc cacggtgcgg gtcatgtccg agacgtagcc gttgtacacc
     1201 gcgccgaagt ccattgtgat gaagtcgccc ggctgtacga ctctgtcgga gaagatgccg
     1261 tgcggacgcg cgcccctagg tccggaggcc acgacgaagt gccccttcgc catttgctgc
     1321 gctcccctgc ggcggatttc gtggacgagg tcggcggcga gctccgcttc ggtcctgccc
     1381 ggacggatcg ccggaaggaa agcctcgaag gcctccgccg caatccgggc cgagcgccgc
     1441 agtagctcca gctcctcggc gtccttgacg gcgcggagct gctgtacaag gcgcgttgtg
     1501 ccgatcagcg aggggagcag gttttcaagc gcgcgccccc gttcgaggct gagcttgtcg
     1561 tcctccaccg cgagccgccg cacgcccagc tgccggagcg cctcccgcac ggcgggccac
     1621 gggtcgcggt cctccatccg tacaaggggg aggaaggatt cctcctcggc ctggatgaag
     1681 tagcgcgaat ccgtgaccag acgcccgccg ttcacccccg ccacaaccac ggcgaaggag
     1741 cctgtgaacc ctgaaaggta ctgcaccgcg ggacggttat ccagttcgct gctgaagacg
     1801 aagagggcgt cggcctctct cctccggagt tcttccgcca ggcgctccat ccgcgccgcg
     1861 aacatactgt tcatctctcg cacacgaccg ctccccttct ttgaaatagg atacggaaca
     1921 gtatgcatta taccaagtcg atgatgaggg tgaaagggtt ttcttttact cggagaaaag
     1981 ttctttcgtc aactaagtgt ccggacaagc ctcaggagcg tgctccgggg gagagacgcc
     2041 tcggacgagc gtcgtcggcg cttctctccc gactgttttt cttcgtcttc agggggtccg
     2101 gacgacgggg cgcccgatga ccgggataag gtcgtggatc gtctccgcct tagacttaaa
     2161 gttttccagc gcctcgaagc tgagcgccca gacgtccatc ggcatggagc cgcgtatcag
     2221 gtcgagcccg aacggggcga gaagcgagac gcacgcttcg gcctcctcct cgcgctcgaa
     2281 gaggaagagc agccagttga tgccgtcctg gtcgaagctg tcgaggtcga tatagccggg
     2341 gagcaccatg cggcggagca cttcgagcgc gagctgaaag cgtcctatcg tcgcgagaaa
     2401 tccgtcgaga ttccccgctt gaggcgcttg cgcctgcaag cagaagaagc ggtccgcgtt
     2461 cagtattccc cacgggtcgt tgggggagcg ggggatgtgc gcgtacgcct ggaagtcgta
     2521 gcggctgagg tggagcaggt ccgcctcgaa gtacgcgggg tagctcccct tcgcctcgaa
     2581 ccacttcgcg gcggcgtccg ggggaggcgg cgtttccttt cgggcggcga gtcccgtaac
     2641 aggccagtcc tcgcgcatct gtccgataat cggcgaagag ggccagatcc attcgaaaac
     2701 ggacgagcgg gtgaagagct cgtcgtcctg cagcggcggg gtcgggaagc gcgcggacag
     2761 gacaaactcc ttcacacccc agcgctggat gtgtttcttg tcgagcagca ggcggaggcg
     2821 cgttctgaaa aggtcctttt ccttgtccgc tccgggaatt cccggtacgg cgggcaggta
     2881 gttcgccgcc gcctcctcgc tgaaggcttc tctccccgcg ccggtcaggg agaaaagctc
     2941 gccctccttt gcgagcagcc cttcgccgag catcagtgcg agcggccctg gatcctcttc
     3001 ggcg
//
LOCUS       DAOTIL010000268         3001 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminobacteriaceae bacterium isolate AMR_MDS_3026
            NODE_38864_length_3001_cov_3.339783, whole genome shotgun sequence.
ACCESSION   DAOTIL010000268 DAOTIL010000000
VERSION     DAOTIL010000268.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868050
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminobacteriaceae bacterium (wastewater metagenome)
  ORGANISM  Aminobacteriaceae bacterium
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae.
REFERENCE   1  (bases 1 to 3001)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3001)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:19:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,224
            CDSs (total)                      :: 3,198
            Genes (coding)                    :: 3,184
            CDSs (with protein)               :: 3,184
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3001
                     /organism="Aminobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_38864_length_3001_cov_3.339783"
                     /isolate="AMR_MDS_3026"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:3034609"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(151..1842)
                     /locus_tag="P5201_06540"
     CDS             complement(151..1842)
                     /locus_tag="P5201_06540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013048955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006826 - iron ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HRV98228.1"
                     /translation="MTAFARGRREIQLLTRDPLLALLVVLLFVSLGVFVVFPLLSVLV
                     KSFQSEQGAFSLENYTRFLTFSYLRSSLWNSLTVGFCTAACSVLVGFAAAFTITRTSV
                     PWKKALHLLFILPIISPPFTSALSILMLFGANGLITRGLLGIRNYNIYGFKGVLLSQI
                     FTFAPVAYLTLKGVMESLNPTLEDAAMNVGAGRLQTFLRVTLPLSLPGIASAFLVVLI
                     ESLADFGNPPVLAGSRFPMLSTQAYLEITGSFNLPLGAALAVVLLIPSITSFAIQRYY
                     LQKRQYTTVTGKPVASSSKLVSRNARRGLLAFSAAFCGLVLLFYGTIFVGAFTRVWGF
                     DFTPTLNHFAYAFSVGMGTIRDTLIVAICATPLSGVLGMLIAFLVVRTSFPGKGVMEF
                     TSILNFAVPGTVIGIGYILAFNTPPLMLIGTLAILVLNFVFRYIPVGIQSGIAVLRQI
                     DPSIEEAAQNLGADKLTTFRKVTLPIIAPAFFSGLVFAFVRAMTAISAAIFLVSANWN
                     LLTVQILNQVGSGRLGVAAAFSVILVVIVTAAMAIIARLVPGRAGGMAAIQIQEE"
     gene            complement(1915..2925)
                     /locus_tag="P5201_06545"
     CDS             complement(1915..2925)
                     /locus_tag="P5201_06545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005659748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HRV98229.1"
                     /translation="MKRYGKLVCIATAALLAFAFLGGAAFAKELVVYSSVDEENAKNL
                     LDEFSKATGITVNMVFLSSGPAMSRIEAEQANPQADVWFGAPNENHIIAKDRGLTQPY
                     VSAAAGDLASGFKDPEGYWHAFYMNPLGFGVLPEELKADGKSVPASWKDLLDPAYKGM
                     IQMPSPQSSGTAYAMIMTLIETFGENEAYAYMKELNPNIQTYTQSGTGPSKNLAIRET
                     SIAIQFTPAFLKLVDEGYPAKVVFPAEGVGYEAAALSIIKGAKNLDSAKALVDWIVSK
                     EGQQVLSAKKTYFFPVRTDVSAGEGLPPLSEIKLIDYDRLEAAREKKRLVDRWVTEVL
                     GQ"
BASE COUNT          596 a          888 c         1018 g          499 t
ORIGIN      
        1 gttttgccgc accccgaggg accgagaaga gtgaccagct cgccgtcgcg gacctccagc
       61 gagacggcgt ccaccgcccg gaagatgctg ccggggtcgt cgaagctgcc gaagtccttg
      121 gttatggagt cgatgactat tttcacgagc ctattcctcc tgaatctgga ttgccgccat
      181 gccgcccgcg cggccgggga cgaggcgcgc gatgatcgcc atcgccgccg tcacgatcac
      241 cacgagaatc acgctgaatg ccgcagccac gccgagacgc cccgatccca cctggttcag
      301 tatctgcacc gtgagcaggt tccagttggc cgagacgagg aagatggcgg cgctgatggc
      361 ggtcatcgcg cggacgaagg cgaagacgag ccccgagaag aaggccgggg cgatgatcgg
      421 cagcgtgacc ttccggaagg tggtcagctt gtccgcgccg aggttctgcg cggcctcctc
      481 gatggacggg tcgatctgac ggagcaccgc gatccccgac tggatgccga ccgggatgta
      541 gcggaagacg aagttcagca ccaggatcgc gagcgtgccg atcagcatga ggggcggcgt
      601 attgaaggcg aggatgtacc cgatgccgat cacggtgccg gggacggcga agttgaggat
      661 ggaggtgaac tccatgaccc ccttgccggg gaacgaggtg cgcaccacga ggaaggcgat
      721 cagcatcccc agcacgccgg agagcggcgt ggcgcagatc gcgacgatga gcgtgtcgcg
      781 gatggtcccc atgccgacgc tgaaggcgta ggcgaagtgg ttcagcgtag gagtgaagtc
      841 gaacccccag acgcgggtga acgcgccgac gaagatcgtc ccgtagaaga ggaggacgag
      901 gccgcagaag gcggcggaga aggcgaggag tccgcgtcgt gcgtttctgc tcaccagctt
      961 ggaggaggag gcgacgggct ttccggtcac ggtggtgtac tggcgcttct gcaggtagta
     1021 gcgctggatg gcgaaggagg tgatcgacgg gatcagcagc accaccgcga gagcggcccc
     1081 gagcggcagg ttgaacgagc cggtgatctc gaggtaggcc tgggtggaga gcatggggaa
     1141 gcggcttccg gcgagcaccg gcgggttgcc gaagtcggcg agcgactcta tgagcaccac
     1201 aaggaaggcg ctcgcgattc ccggcagact cagcggcagc gtcacgcgca gaaaggtctg
     1261 gagcctcccc gcgcccacgt tcatcgccgc gtcctccagc gtcggattca gcgactccat
     1321 gacgcccttc agcgtgaggt aggccaccgg ggcgaaggtg aagatctgcg acagcagcac
     1381 gcccttgaag ccgtagatgt tgtagtttcg gattcccagc agcccccgcg tgatcaggcc
     1441 gttcgcgccg aagagcatca ggatggagag cgcgctcgtg aaggggggcg agatgatggg
     1501 gaggatgaag agcagatgga gcgccttctt ccaggggacg gacgtgcgtg tgatcgtgaa
     1561 cgcagccgcg aagccgacga gcacgctgca cgcggcggtg cagaagccga cggtgagact
     1621 gttccagaga gacgagcgaa ggtagctgaa cgtgaggaag cgcgtgtagt tctcgagcga
     1681 aaacgcgccc tgctccgact gaaagctctt gacgagcacg gagagcagcg ggaagacgac
     1741 gaagacgccc agcgacacga agagcaacac taccagaagc gcgagaaggg ggtcgcgggt
     1801 cagaagctga atctcccttc tcccgcgtgc gaatgcggtc attctgttga ttgacacctc
     1861 ctaagcaaaa gggggaagag gcaagcccct tcccccgcag ctacaacaca acgattactg
     1921 gccgaggact tccgtcaccc agcggtcgac gaggcgcttc ttctcgcgcg cggcctcaag
     1981 acggtcgtag tcgatcagct tgatctcgga gagcggggga agcccttcgc ctgccgagac
     2041 gtcggtgcgc acggggaaga agtaggtctt cttcgcgctg agcacctgct gcccttcctt
     2101 ggagacgatc cagtcgacaa gcgcctttgc gctgtcgaga ttcttcgccc ccttgatgat
     2161 ggagagcgcc gccgcctcgt accccacgcc ctcggcgggg aagacgactt tcgccgggta
     2221 cccctcgtcc accagcttca ggaacgccgg ggtgaactgg atggcgatgg acgtctcgcg
     2281 gatggcgagg ttcttgctgg ggccggtgcc gctctgcgtg tacgtctgaa tgttcggatt
     2341 gagttccttc atatacgcgt acgcctcgtt ctcgccgaac gtttcgatca gggtcatgat
     2401 catggcgtag gccgtgccgg acgactgcgg cgagggcatc tgaatcatcc ccttgtacgc
     2461 cgggtcaagc agatccttcc acgacgccgg aacggacttg ccgtcggcct tcagctcctc
     2521 ggggagcacg ccgaagccga gcggattcat gtagaacgcg tgccagtacc cctcgggatc
     2581 cttgaagccg gaagcaaggt cgcccgccgc cgcggagacg tacggctgcg tcaggccgcg
     2641 gtcctttgcg atgatgtggt tttcgttggg agcgccgaac cagacgtccg cctgcggatt
     2701 ggcctgctcg gcctcgatgc ggctcatcgc cggaccggag gagagaaaga ccatgttcac
     2761 cgtgatgccg gtcgccttcg agaactcgtc gaggaggttc ttcgcattct cctcgtccac
     2821 gctggagtag accaccagtt ccttcgcgaa cgccgcgccg ccgaggaacg cgaacgccag
     2881 aagcgccgcg gtcgcgatgc acaccagttt tccgtaccgt ttcattcttc aaatccctcc
     2941 cgaatcagag tgtaggactg cccaagtgtt gcggaaatct tgcagtattg taacacaaag
     3001 a
//
LOCUS       DAOTIL010000269         2996 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminobacteriaceae bacterium isolate AMR_MDS_3026
            NODE_38961_length_2996_cov_3.410405, whole genome shotgun sequence.
ACCESSION   DAOTIL010000269 DAOTIL010000000
VERSION     DAOTIL010000269.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868050
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminobacteriaceae bacterium (wastewater metagenome)
  ORGANISM  Aminobacteriaceae bacterium
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae.
REFERENCE   1  (bases 1 to 2996)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2996)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:19:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,224
            CDSs (total)                      :: 3,198
            Genes (coding)                    :: 3,184
            CDSs (with protein)               :: 3,184
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2996
                     /organism="Aminobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_38961_length_2996_cov_3.410405"
                     /isolate="AMR_MDS_3026"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:3034609"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..461)
                     /locus_tag="P5201_06550"
     CDS             complement(<1..461)
                     /locus_tag="P5201_06550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013047671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HRV98230.1"
                     /translation="MTGRLIPAAATLSFALCWEAFCRLQEIPHFILPAPSRIAILLLT
                     EAPLLLKHAAVTTFEIALGIVFSLAAALPLSIAMFFSPLLERALSPLLIASQAIPVFA
                     VAPLLVVWFGYGTGSKVIMAALIIFFPVTVTLLQGFKSCDPGYQRLFFVMGA"
     gene            complement(461..1192)
                     /locus_tag="P5201_06555"
     CDS             complement(461..1192)
                     /locus_tag="P5201_06555"
                     /EC_number="7.6.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013047672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HRV98231.1"
                     /translation="MLEIRHFSKDFNGLPVLSNFSLALPRNSFTVLVGPSGCGKSTLF
                     DLLMGSVSRDGGRLFLFGAELPDLRGEAGYMAQKDLLLPWLSLIQNALLPVMVKRKLT
                     SDDYKEAKNLFSSLELSGFENHYPRQVSGGMAQRCALARTYFFRAPLALLDEPLSAVD
                     ALTRQSLRTMLLLLQSRFEKTILMVTHDVDDALFLADRIVVLSRSPMMVTQEFIPEGK
                     KPRNPSNTELLHLKEHILRSLQEGR"
     regulatory      complement(1246..1355)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            1749..2279
                     /locus_tag="P5201_06560"
     CDS             1749..2279
                     /locus_tag="P5201_06560"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRV98232.1"
                     /translation="MEVFDASSALAAAAAEYPGRKIVLWGFSLGGLLSVLLAGGESGR
                     FIRQTGLEPITLPGVDALVISGSGDRIRAEASAGLALPILNSLGSSEVIHTAASKVSL
                     SFALFFYGGCDGTFDEESSRRILRSLRLDEEKKEFHILPGVDHAFRTVNGTPSFEPLE
                     QMLGITMATLERRRRT"
     gene            complement(2242..2994)
                     /locus_tag="P5201_06565"
     CDS             complement(2242..2994)
                     /locus_tag="P5201_06565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014807512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase"
                     /protein_id="HRV98233.1"
                     /translation="MVFFSFLGGSFGSFLNVVALRSISGKSWGGGERSVCVSCGTVLE
                     WRDLVPLVSWCMLRGKCRRCGTRIGIRYLAVEVIGALAGGLLAWRWGVSYTLLLALAA
                     TFGLFLNALTDVEDGYVFDVFPLTMGIIGVALRLPGGWDALFDGLLGAGAGFAVIACI
                     ILISRGGMGWGDATLAAGTGSIMGWKMLLLTLYSGFMAGGILALLLLLAGKVRRKDAI
                     PLVPFLALGGFFTLLAGPRILMFFGAAPGWPW"
BASE COUNT          764 a          803 c          849 g          580 t
ORIGIN      
        1 gctcccataa caaaaaacag cctttgatac cctggatcgc agcttttaaa accttgcagc
       61 agcgttacag tgacagggaa gaaaataatg agcgccgcca tgataacctt gctgcccgtt
      121 ccatagccga accaaaccac cagaagtggc gccacggcga agacgggaat cgcctgtgag
      181 gctattaaca acggagaaag agctcgttca agaagaggcg aaaagaacat cgctattgag
      241 aggggaagag cggccgcaag agaaaataca atcccaagag cgatctcgaa tgtcgtcacc
      301 gcagcgtgtt tcagcagcaa cggcgcctcc gtcagcagaa gaatcgcgat tcgagagggg
      361 gcgggaagaa taaagtgggg gatctcctgc agacggcaga aagcttccca gcacagcgca
      421 aacgagagcg ttgcagcagc gggaatgagg cggccggtca tcaacgtccc tcctgaaggg
      481 agcgcagaat gtgttccttc aggtgcagga gttccgtatt cgacggattc cgcggcttct
      541 tgccttccgg aatgaactcc tgggttacca tcatcggaga gcgagaaaga acgacgatcc
      601 tgtcggccag gaaaagagcg tcgtcgacat catgcgtaac catcaggatc gtcttttcga
      661 aacgtgattg cagcaagagc agcatggtgc ggagcgactg ccgggtaagc gcgtcaacag
      721 cggaaagagg ttcgtcgaga agcgcgaggg gagcccggaa aaagtaggtc cgtgcaagag
      781 cgcatcgttg cgccatgcct cccgaaactt gtcgggggta gtgattctca aagcctgaga
      841 gctccaggga gctgaagagg ttttttgctt ctttatagtc atctgaagtc agctttcttt
      901 tcaccataac agggagaagc gcgttttgaa taagcgaaag ccatgggagc agcagatctt
      961 tttgtgccat gtatcccgct tctccgcgaa ggtccgggag ttcagcgccg aaaagaaaga
     1021 gccgcccccc gtctctggat acgctcccca tcaggaggtc gaagagcgtg ctctttccgc
     1081 accctgatgg gccgacgagt actgtaaagc tgtttcgggg cagcgcaaga gaaaaattcg
     1141 aaagaacggg aagcccgttg aaatctttag agaaatgacg tatctcaagc ataaaaaact
     1201 cccggcaagg gatgccggga gtaggagttg cgacttcttc gcggatggca acgaacgcac
     1261 tcttcccttc gccggcatga cccggatcag gttccagggg tcggggccgt tagccccctc
     1321 tcagccggta gatgccgact cccccagttg ctgtcgacaa cagaatacgc ttttcccttt
     1381 gctttgtcaa ttcattgaaa tctgcaagga aaagagagat aatcgtatgc aggatttgaa
     1441 agggggattt cttcgtgaat attcgatgca aaagctgccc gggaagtttt ggtgataaag
     1501 aagttctact gcattatttc gatgaagaga cgagggcgga cgcccctttg ttcctcttgc
     1561 tgcacggagt tcacggatgc gcatcttcgg ttgaagggaa caagtacggg tacctggccc
     1621 gcttgattgc gcacaagagg ttcccggtat gcgccgcaga gtccagccgg ctgcgacggg
     1681 atagagaaac gttcggcgac gatcggcccg catgggcggt ggcttccttc caggggaaaa
     1741 cgttcgctat ggaggttttc gatgcatcct cagcccttgc tgctgcggct gcggagtatc
     1801 caggccggaa aatagtgctc tggggttttt ctctcggagg gcttctgtcg gtactcctcg
     1861 ccgggggaga gagcggaaga tttattcgtc agactgggct ggagccaata acgcttcccg
     1921 gcgtcgacgc cctcgtcatc tccggaagcg gcgaccggat ccgtgccgag gcctcggcgg
     1981 ggctcgcgct tccgatactg aactcccttg gctccagcga agttattcac acggctgcat
     2041 cgaaagtatc gctttccttc gctcttttct tttacggtgg atgcgatgga actttcgacg
     2101 aagagtcgtc gagaagaatt ttacgcagtc tccggcttga cgaggagaaa aaagaatttc
     2161 atatactgcc cggagttgac catgcattcc gtacagtgaa cggaacgccc tcttttgaac
     2221 cgctggaaca gatgcttgga atcaccatgg ccaccctgga gcggcgccga agaacatgag
     2281 aatccggggg ccggcaagga gagtaaaaaa accgcccagc gcgaggaacg gaacaagcgg
     2341 aatcgcatcc ttgcggcgta cttttcccgc aagcagaagg agaagcgcga ggattccgcc
     2401 tgccatgaag cctgagtaga gggtgaggag gagcattttc caccccatta tgctccctgt
     2461 accggcagca agcgtagcat ctccccaccc catgcctcct ctggaaatga ggatgataca
     2521 ggcgattact gcaaaacctg ctcccgcccc gaggagaccg tcaaacagcg catcccagcc
     2581 tcccggcaaa cgcagggcga cgccgatgat tcccatggta agaggaaata cgtcgaaaac
     2641 gtacccgtct tcgacgtcgg tcagcgcgtt cagaaaaaga ccgaaagtag cggccaaagc
     2701 gaggaggagc gtataagaaa cgccccatct ccaggcaagc agccctccgg caagagcgcc
     2761 tataacctct accgccaagt aacgaatgcc gatcctcgtc ccgcagcgac ggcattttcc
     2821 acgaagcata caccatgaaa ccagcggcac aagatctctc cactccagaa ctgttccgca
     2881 ggagacgcac acggatcgtt ctcctccgcc ccaggatttt ccagaaatgc tccgtaacgc
     2941 gacgacgttc agaaacgagc cgaaagatcc gcccaaaaaa gaaaaaaaaa cgatga
//
LOCUS       DAOTIL010000270         2991 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminobacteriaceae bacterium isolate AMR_MDS_3026
            NODE_39057_length_2991_cov_3.400545, whole genome shotgun sequence.
ACCESSION   DAOTIL010000270 DAOTIL010000000
VERSION     DAOTIL010000270.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868050
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminobacteriaceae bacterium (wastewater metagenome)
  ORGANISM  Aminobacteriaceae bacterium
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae.
REFERENCE   1  (bases 1 to 2991)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2991)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:19:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,224
            CDSs (total)                      :: 3,198
            Genes (coding)                    :: 3,184
            CDSs (with protein)               :: 3,184
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2991
                     /organism="Aminobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_39057_length_2991_cov_3.400545"
                     /isolate="AMR_MDS_3026"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:3034609"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..204)
                     /locus_tag="P5201_06570"
     CDS             complement(<1..204)
                     /locus_tag="P5201_06570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014807497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(2Fe-2S) ferredoxin domain-containing protein"
                     /protein_id="HRV98234.1"
                     /translation="MTEEKKKIRSLDDLRKIKEKTGDLTSARSEVDKVKVIIGMGTCG
                     IAAGAREIMNAVLKELEVRNLHNV"
     gene            complement(242..784)
                     /locus_tag="P5201_06575"
     CDS             complement(242..784)
                     /locus_tag="P5201_06575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005659472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HRV98235.1"
                     /translation="MLEDLSHHILDIAENGVNAGAESIGVTFDDDGEFIRLTVRDNGR
                     GMSEATARAVVDPFYTTRTERRVGLGLPFLKQLSELCGGEFLLSSAPGKGTTVSASFS
                     KQCVDTPPPGDIPSTLLTLLVGHGHISWKYVHRVGKREFLLDSDELKDALGGGNPFTD
                     PALAVGVKEYIAENIGELYM"
     gene            complement(787..1266)
                     /gene="nuoE"
                     /locus_tag="P5201_06580"
     CDS             complement(787..1266)
                     /gene="nuoE"
                     /locus_tag="P5201_06580"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014807499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoE"
                     /protein_id="HRV98236.1"
                     /translation="MVVETKQDVRSMVQEITAPWKGKHGGVIPVLQETQKKVGYLSRE
                     AMEVVSEELGVPLAELYGVATFYAQFHLKPRGRHIIRVCRGTACHVRGSAKIMDKIMS
                     MLKVKENGTTDDLRFTLEPVACLGCCGLAPVVMIDEDTHGRLTDDEAALQEFLDQYA"
     gene            complement(1531..2460)
                     /locus_tag="P5201_06585"
     CDS             complement(1531..2460)
                     /locus_tag="P5201_06585"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRV98237.1"
                     /translation="MNTTQKIERLLDEAYTTDDPEEMERLARQILNYDKDNVEAIILL
                     ADVTEDSKEKIVLLQKASCLLEKDLEESPVPEGTAFLDDELGMLYAAVLQRLGFSYFS
                     EGENEKALEIAHSIMAFDTEQQTLGRTLYYRILVDMNEYTAILEECLKEREPFPAMLH
                     SKAIAAFRLSGATMPAYRALWEAFAADPDVPFHILGYYEEPDEEAEEEELESYSLAML
                     FEDAWLTEGDAALANWVAGGTILLGLAASLFPAENSEKMLVLADALGLADAAEDIMVK
                     LESRQDWNVLSKQEKVKEALKLLSEGKYLPLKS"
     gene            2759..>2991
                     /locus_tag="P5201_06590"
     CDS             2759..>2991
                     /locus_tag="P5201_06590"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid-binding SYLF domain-containing protein"
                     /protein_id="HRV98238.1"
                     /translation="MGELIAQARGVAIFPSVVKAGFVFGGQYGEGLVLRRDPVKKRWY
                     GPSFVSVTGASWGLQIGAQSIALVLVITNDRGL"
BASE COUNT          727 a          777 c          675 g          812 t
ORIGIN      
        1 gacgttgtgc agattgcgaa cttcaagctc tttaagcacc gcgttcatta tttcgcgggc
       61 gccggcggcg ataccgcacg tacccatacc gatgatgacc ttgactttgt ccacctcgga
      121 acgcgcggat gtcaaatcgc ccgttttctc tttgatcttt cgcagatcgt cgagactccg
      181 gatctttttc ttttcctcag tcatttcttt ttcctcctgt ccgcattatt tcccattttt
      241 ttcacatata aagctcgccg atattttccg cgatgtactc ctttactcca acggcaagcg
      301 cgggatctgt gaaagggttc ccgcccccga gcgcatcctt cagttcatcg ctgtccagaa
      361 ggaactccct cttgccaact ctgtgaacat atttccagga gatatgcccg tgaccgacaa
      421 gaagagtaag cagagtcgag ggaatatctc cgggaggagg agtatctacg cactgtttgc
      481 tgaatgacgc cgaaacagtt gttccttttc ctggcgctga agaaagaagg aattcaccac
      541 cgcataactc agaaagttgt ttaaggaaag gaagtccaag acccacccgg cgctccgtcc
      601 gcgttgtgta gaaaggatcc actacagcgc gggctgtcgc ttcgctcatt cccctgccgt
      661 tatcgcgaac ggtcaaacga atgaactcgc cgtcgtcgtc aaatgtaaca ccgatcgatt
      721 cggctccggc attcacgcca ttctcagcga tatcgagaat atggtgggag agatcctcaa
      781 gcatgatcag gcgtactgat caaggaattc ttgcaaggcc gcttcgtcgt cggtgagtct
      841 gccgtgagta tcctcgtcga tcatcactac aggagcaagg ccgcagcaac caagacacgc
      901 gactggttca agtgtaaaac gaagatcatc ggtggttccg ttttccttta ccttgagcat
      961 cgacatgatt ttgtccatga tctttgcgct gccgcgaaca tgacacgcgg ttccgcggca
     1021 cactcggata atatggcgtc ccctgggttt cagatgaaac tgcgcataga aggttgcaac
     1081 accgtaaagc tccgcaagag gaacgcccaa ctcctccgaa actacctcca ttgcctcccg
     1141 ggacaagtac cctacttttt tctgcgtctc ctgaagcacc ggaataacac ctccgtgctt
     1201 tcctttccac ggagcggtaa tttcctgtac catggaacgc acgtcctgct ttgtttccac
     1261 aaccattgcc cgacccccta agtatagtga atttaataac atatagaata acaaaaaact
     1321 atctcttgtt gattctacac aaaaaaacaa cttcggggaa agaaaccgtt ttcctgtaaa
     1381 cggcgcccga tgtaaatatt accacagaaa aaagattttt tctctaaaaa agcaagtttt
     1441 tgctttatct catacatctt ctcgatacgt agtcttcttg ctggatcctt ttttacaaaa
     1501 acacatctaa ttcaataaaa aataagagca ttaagatttc agtggaagat atttaccttc
     1561 agagagcagc tttaatgcct ccttcacttt ttcctgtttt gaaagaacat tccagtcctg
     1621 tctgctctcc aactttacca ttatgtcctc agcagcgtcg gcgagaccga gggcatctgc
     1681 aagcaccagc attttctcgg aattctctgc cgggaagagc gatgcggcca gaccaagcaa
     1741 gattgtccct ccggcaaccc aatttgccaa cgcggcgtct ccttcagtca accacgcatc
     1801 ttcaaagagc attgcgagac tgtagctttc cagttcctcc tcttcagcct cttcatccgg
     1861 ttcttcatag tagccgagaa tgtggaaggg aacgtcggga tccgccgcga acgcttccca
     1921 aagcgcccta taggccggca tggtagctcc ggagagccta aacgcagcta tagctttact
     1981 gtggagcatt gcaggaaaag gctctctttc tttcaagcac tcctcaagga tcgccgtgta
     2041 ttcgttcata tcgacaagga tacgatagta aagagttctt cccagcgtct gttgttccgt
     2101 gtcaaatgcc atgattgagt gcgcaatttc aagcgctttt tcgttctcgc cttcggagaa
     2161 ataggaaaaa ccgaggcgtt gaagaactgc tgcgtagagc attccgagtt cgtcatccag
     2221 aaaggctgtt ccttccggaa cgggcgattc ttccagatct ttctccaata agcaacttgc
     2281 tttttgcaaa aggacgatct tctccttcga gtcctcggtg acatcggcca aaaggatgat
     2341 tgcttccaca ttatctttgt cgtaattcaa aatttgtcga gcgaggcgtt ccatttcctc
     2401 cggatcatcg gtagtatacg cctcgtcaag aagtctttcg attttctgtg tagtattcat
     2461 agatatttcc tccgtttgct ttttcagtct cttccctctc caagaagtct agctcacgat
     2521 tttttcccgg tcaaataggt tggtgtagaa tatgaagaag tgcaaaaaac catgaatctt
     2581 ttttctagga ggagagggaa tgagacgggt gcgcgtatct ttttctttgt gtgttgtctt
     2641 cttgttactt atcttgaatt tgacggaaag gccatcgttc gcttcaactc caaaaggtcg
     2701 aattgccgat tccgtaaaag tgcttcgtga aatgtcgaaa caggatgatg catcgacaat
     2761 gggagagctt attgcgcagg ccaggggagt cgctattttc ccttccgtcg taaaagctgg
     2821 gttcgttttc ggaggacagt acggcgaggg ccttgtcctt cgccgcgacc cggtaaaaaa
     2881 aagatggtac ggtccatcct tcgtaagcgt taccggggcc tcctggggat tacagattgg
     2941 agcgcagtcc atagcgcttg ttctcgtaat caccaacgac agggggctgg a
//
LOCUS       DAOTIL010000271         2984 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminobacteriaceae bacterium isolate AMR_MDS_3026
            NODE_39187_length_2984_cov_2.448276, whole genome shotgun sequence.
ACCESSION   DAOTIL010000271 DAOTIL010000000
VERSION     DAOTIL010000271.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868050
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminobacteriaceae bacterium (wastewater metagenome)
  ORGANISM  Aminobacteriaceae bacterium
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae.
REFERENCE   1  (bases 1 to 2984)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2984)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:19:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,224
            CDSs (total)                      :: 3,198
            Genes (coding)                    :: 3,184
            CDSs (with protein)               :: 3,184
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2984
                     /organism="Aminobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_39187_length_2984_cov_2.448276"
                     /isolate="AMR_MDS_3026"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:3034609"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1353)
                     /gene="ilvD"
                     /locus_tag="P5201_06595"
     CDS             complement(<1..1353)
                     /gene="ilvD"
                     /locus_tag="P5201_06595"
                     /EC_number="4.2.1.9"
                     /inference="COORDINATES: protein motif:HMM:NF013113.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004160 - dihydroxy-acid dehydratase
                     activity [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0009097 - isoleucine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009099 - valine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxy-acid dehydratase"
                     /protein_id="HRV98239.1"
                     /translation="MTNKWRSSEVFAGAPNAGARALWKSMGYCDGDFAERPVIGIANS
                     WNTLVPGHFNLNQVAEQVKKGIHRAGGVAAEFGVIASCDGVCEGHRGMHFILPQREVI
                     ADSVELVVEAHHMDAIVLLASCDKIVPGMLMAAARLDIPAIMITGGPMLGGVEFDGRK
                     TDETSLHEALGMLTAGRISDEEFHSLENLCAPTCGSCSFFGTANTMCCLAEVLGMTLP
                     GSALVPAVHAERLRLACATGERICEMAREGLTARKILNASSIVNGVRATLAMSGSTNA
                     VMHLAAIAAEAEAGVDVMELFSRLEQETPQIVRINPASKWNTEDFWMAGGIPRMLKNM
                     LPIIEKDALTCTGRTVEENVKAFRFPFPKNNEVMKTLESPFSKRGGIAVLRGNLAPDT
                     GVTKPGAIDPTLHVFTGEARVFDSEDDANAAILAGKIHKGNVVVIRYEGPKGGPGMRE
                     M"
     gene            1534..1992
                     /locus_tag="P5201_06600"
     CDS             1534..1992
                     /locus_tag="P5201_06600"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRV98240.1"
                     /translation="MMRCRIIAWTLLLFFAASCVPAESVMPPEAYANMAEKSRIKATA
                     VVESVKALETGKQSTWKSVVFKLRFPFTENVPEQFTGTCYSVDFPWQEPPVGGTLYYY
                     PEAGDLVYVTVGSNGGPITSYTYLNDVMESLFIDNEEKIRYGMGNAWLDL"
     gene            2029..2937
                     /locus_tag="P5201_06605"
     CDS             2029..2937
                     /locus_tag="P5201_06605"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRV98241.1"
                     /translation="MRRASRGRCVACFLLSVVIVFFAAAGPSSADYSTTGVVGGPHPF
                     TYTVKCAPGDRFEVAVSSDYATSVDILALTPDSRAHGGWAVNSVASSRRKAAHSLSYA
                     APAGKPENNATHWHYKISILASTQKQTKFMLKITQYGSGASRSKAYVEQVNKQLQALG
                     RAMKNEQNRLNTEIKQRSERIKNIDSEQKRKKERLVAELAELERLNESLRNEKNKAVR
                     SSMQQTLKSRTDRYNASVESYNKTRAARKALYDEYKALGERRSEIDNLREALREALGR
                     NDYDRCVIIANGSRLALSLGWKTMKR"
BASE COUNT          611 a          860 c          933 g          580 t
ORIGIN      
        1 catctcgcgc attccggggc cgcccttcgg cccctcgtag cggatgacca cgacgtttcc
       61 cttgtgaatc ttcccggcga ggatggcggc gttcgcgtcg tcctcgctgt cgaagacgcg
      121 ggcctccccg gtgaagacgt ggagcgtcgg gtcgatggcg ccgggtttcg tcacgccggt
      181 gtccggcgcg aggttgcccc gcaggacggc gatccctccc cgcttcgaga agggggactc
      241 gagcgttttc atcacctcgt tgttcttcgg gaaggggaag cggaaggctt ttacgttttc
      301 ttccacggtg cgccccgtac aggtcaaggc gtccttttcg atgatcggga gcatgttctt
      361 cagcatccgg gggatgcccc cggccatcca gaagtcctcc gtgttccact tcgacgcggg
      421 gttgatccgc acgatctgcg gcgtctcctg ttcaaggcgg gagaagagct ccatcacatc
      481 gacgccggct tccgcctccg cggcgatggc ggcgaggtgc atcaccgcgt tggtcgaacc
      541 gctcatggcg agcgtcgccc gcacgccgtt cacaatggag gaggcgttca gtatcttccg
      601 cgccgtcaac ccctcgcggg ccatctcgca gatacgctct cccgtggcgc aggccagtcg
      661 cagccgttcc gcgtggaccg cgggtaccag cgcgcttccc ggaagcgtca tcccgaggac
      721 ttcggcgagg cagcacattg tgttcgcggt gccgaaaaag gagcaggagc cgcaggtggg
      781 tgcgcagagg ttttcgagcg aatgaaactc ctcatcggag atgcgccccg ccgtcagcat
      841 tcccagcgcc tcgtggagcg acgtttcgtc ggtcttgcgt ccgtcgaact ccacgccgcc
      901 gagcatcggt ccgccggtga tcatgatcgc ggggatgtcg agccgggccg ccgccatcag
      961 catgccgggg acgatcttgt cgcacgaggc cagcaggacg atcgcgtcca tgtggtgcgc
     1021 ctcgacgacg agctcgaccg agtcggcgat gacctcgcgc tgcggaagga tgaagtgcat
     1081 ccccctgtgc ccctcgcaaa cgccgtcgca cgatgcgatg acgccgaact ccgccgccac
     1141 ccctcctgcg cgatggatcc ccttcttcac ctgttcggcg acctgattca ggttgaagtg
     1201 ccccggaacc agcgtgttcc acgagttcgc gatgccgatg accggccgtt cggcgaaatc
     1261 accgtcgcag taccccatcg acttccacag cgcccgcgcc ccggcattcg gcgcacccgc
     1321 aaagacctca gagcttctcc atttatttgt catgaacggt cctcctccgg aaaattgcaa
     1381 ttcttctgca tagtagggga gagcgggcgt ttcgtcaaca ggctgcgaaa atccaaaaaa
     1441 gcgaaaatca cgatgcgaat atcctcagga gaggctatac tgaaaaagac ttcgcgatgc
     1501 gattagaaat ccgaatgaga ggagggagga ttcatgatgc gttgccggat tattgcgtgg
     1561 acgctgcttc tgttctttgc ggcttcatgc gtccccgcgg agagcgtgat gcctccggag
     1621 gcttatgcga acatggccga aaaatcgagg atcaaggcga cggcggttgt cgagtccgtg
     1681 aaggctttgg agacggggaa gcagagcaca tggaagagcg ttgtcttcaa actgagattc
     1741 ccgttcacgg agaacgtgcc ggagcagttc accggaacct gttactccgt cgacttcccc
     1801 tggcaggagc cgcctgtggg cgggacgctc tattactacc ctgaagcggg ggatctcgtc
     1861 tatgtcacgg tggggtcgaa cggcggtccg atcacgagct acacgtacct gaacgatgtg
     1921 atggagagcc tctttatcga caatgaagaa aaaatacgct acggcatggg gaacgcctgg
     1981 ctggatcttt aatgtgcagc caggggcgtt tccggaaagg agatgggcat gagaagagcg
     2041 agtagaggac gatgtgtcgc gtgtttcctg ctttcagtgg tgatcgtgtt ttttgcggcc
     2101 gcagggcctt cttcggcgga ctactcgacc acaggcgtcg tcggcggtcc gcaccctttt
     2161 acatatacgg tcaagtgcgc tccgggggac cggtttgagg ttgcggtgtc atccgattac
     2221 gctacctcgg tggatatact cgcccttacg cctgattcgc gggcgcacgg agggtgggcg
     2281 gtcaactccg tcgcgtcgag caggcggaaa gcggcgcatt cgctgagtta tgcggctccc
     2341 gcgggaaagc ccgagaacaa tgcgacgcac tggcactata aaatttcgat tttggcctca
     2401 acgcaaaaac agaccaaatt catgttgaag ataacgcagt atggaagcgg ggcgtcccgt
     2461 tccaaagcat atgttgaaca ggtgaacaag caactgcagg ctttggggag ggcgatgaag
     2521 aacgaacaaa atcgcctgaa caccgaaatc aagcagcggt cggagaggat caaaaacatc
     2581 gactccgagc agaaacgcaa gaaggagcgc ctggttgcgg agctggcgga gttggagcgg
     2641 ctcaacgaat ctctccggaa cgagaaaaac aaggctgtgc gctcttcgat gcagcagacg
     2701 ctgaaatctc ggaccgaccg ttacaacgct tccgtcgagt cgtataacaa aacgcgagcg
     2761 gcccgaaagg ccctctacga tgagtataag gctctggggg agcggcgaag cgagatcgac
     2821 aacttgaggg aagcgctcag agaagcattg gggcgtaacg attacgaccg ctgcgtcatc
     2881 attgccaacg gaagccggtt ggcgctcagc ctcggctgga agacaatgaa gagatgaaat
     2941 ccgggggtga ggcgcgggtg atccgcgttt catccccctt ttcc
//
LOCUS       DAOTIL010000272         2966 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminobacteriaceae bacterium isolate AMR_MDS_3026
            NODE_39458_length_2966_cov_3.907592, whole genome shotgun sequence.
ACCESSION   DAOTIL010000272 DAOTIL010000000
VERSION     DAOTIL010000272.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868050
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminobacteriaceae bacterium (wastewater metagenome)
  ORGANISM  Aminobacteriaceae bacterium
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae.
REFERENCE   1  (bases 1 to 2966)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2966)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:19:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,224
            CDSs (total)                      :: 3,198
            Genes (coding)                    :: 3,184
            CDSs (with protein)               :: 3,184
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2966
                     /organism="Aminobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_39458_length_2966_cov_3.907592"
                     /isolate="AMR_MDS_3026"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:3034609"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..656
                     /locus_tag="P5201_06610"
     CDS             <1..656
                     /locus_tag="P5201_06610"
                     /inference="COORDINATES: protein motif:HMM:NF012772.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HRV98242.1"
                     /translation="MRQDGGMLAPCDFVPQAEHTGFIPNIDICMMEHALRAIVRTQDM
                     ARTSRLFFSANGSPLFFRGLASMETVEFLIDTTGADPKLFTIEVTESALIDNLDDVHR
                     KLDYLKSCGVKIALDDFGTGYSSLQYINYLPFDYLKLDRAFVSQLFSSEKTEHMMRTI
                     IGLAHNLRMDIVAEGVETTEQLSWLRAAGCLKAQGFYFSRPVAWAEAEAMIRKLPEA"
     gene            complement(766..1872)
                     /locus_tag="P5201_06615"
     CDS             complement(766..1872)
                     /locus_tag="P5201_06615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008712886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HRV98243.1"
                     /translation="MSEVILKNVTKKFSTRTLGEVTAVDDFNLTIREGECFSLLGPSG
                     CGKTTTLRMIAGFEDLSDGEIWIGDKPVSAKKRNLYVQPEDRGLGMVFQAFAVWPHLN
                     VFENVAFPLRVRNVGRREMDSRVAEALKHTGLGGLDRTFPSDLSGGQLQRIALARAIV
                     MNPKVLLLDEPLSNLDPKLRESMRFEIKELQRRFNFTIIYVTHDQAEAMALSDRMLVM
                     DMGVIQQIDTPTNMYNNPANKFVYGFLGLSNFVDVNISDGKVYPEGGSGQPFPCAIPG
                     DFTEAAGTVASRPNEIDLTAEGGYRTVVKARIFLGDYTEYRVDVGNQEVRIQSTGDRG
                     FMVGDSVGLDFTRVQWYSRDDGISDAERERRKVI"
     gene            complement(1869..>2966)
                     /locus_tag="P5201_06620"
     CDS             complement(1869..>2966)
                     /locus_tag="P5201_06620"
                     /inference="COORDINATES: protein motif:HMM:NF012738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HRV98244.1"
                     /translation="PLVVPAVAAGAMLILISSLSHFGVPAMLGFSRGIFTLPTKIYEL
                     INRSAGSFEGIRQGAALSILLVVVVVIALWLQRKILKAGHYDIIRGKSMRPMLINLRT
                     SKFPLLGVCLASLFVIVILPLYIIFAVGGLKAYGLPLNLANMTLDNYYHVLFSSRMAQ
                     DAIKNSFFLAISSAIIAMFVGTMIAYVVVKVKVKGKSLLEILALLPYSVPGIVLAIGV
                     ILMWSGTFYLNLYNTIWIILIAYIARYVAFAMKSSSASLEQVHYSLEEAARSCGATHL
                     ESLRDITIALIRPGMIAGFFLIFLPAMRELTTSILLYGPFSRTLGVAIYAINAEGNIT
                     QSAALSGIAILIIMLGNLALRFVTKDRRSVS"
BASE COUNT          611 a          938 c          883 g          534 t
ORIGIN      
        1 ggatgcgcca agacggagga atgcttgctc cctgcgactt cgtcccgcag gcggagcata
       61 ccggtttcat cccgaacatc gatatctgca tgatggaaca tgcgctccgc gcgatcgtgc
      121 ggacacagga catggcgcgc acctcgcggc tcttcttctc ggcgaacgga tcgccgctct
      181 tcttccgcgg actcgcctcg atggagacgg tggagtttct catcgacacc accggcgcgg
      241 acccgaagct cttcaccatc gaggtgaccg agagcgccct gatcgacaac ctcgacgatg
      301 tgcacagaaa gctggactac ctgaagagct gcggcgtcaa gatagccctc gacgacttcg
      361 ggacgggcta ctcctccctc caatacatca actatctgcc gttcgactac ctcaagctgg
      421 acagggcgtt cgtcagccag cttttcagct cggagaagac cgaacacatg atgcgaacga
      481 tcatcggcct ggcgcacaat ctgcggatgg atatcgtcgc cgagggagtc gagacgacgg
      541 agcagctctc ctggctgagg gcggcggggt gtcttaaggc gcaggggttc tacttctcgc
      601 gcccggtggc gtgggccgag gccgaggcca tgatacggaa gcttcccgaa gcgtaaccgg
      661 ccggggagcc ggatgcggaa ggcctgcatc aagctccgga tgggccggcg ccccgtacaa
      721 gcgtacaaaa aagcccccgg agagcgctcc ccgggggcct gtcttctaaa tgacctttcg
      781 tcgttcccgt tccgcgtcgc tgatgccgtc gtcgcgggag taccactgca cacgggtgaa
      841 atccagcccg acactgtctc ccaccatgaa gccgcggtcg ccggtgctct gaatacgcac
      901 ctcctgattc cccacgtcca cccggtattc ggtatagtcg cccaggaaga tgcgcgcctt
      961 gaccaccgtg cggtagcccc cctcggccgt caggtcgatc tcgttggggc gcgacgccac
     1021 ggtaccggcg gcttcggtga aatcgccggg aatggcgcac ggaaacggct gccccgagcc
     1081 tccttcaggg tacaccttcc cgtcgctgat gttcacgtcg acgaagttgg agaggcccag
     1141 gaagccgtag acgaacttgt tcgccgggtt gttgtacatg ttcgtcggcg tgtcgatctg
     1201 ctgaatcacg cccatatcca tcaccagcat cctgtcggag agcgccatcg cctccgcctg
     1261 atcgtgcgtc acgtagatga tggtgaagtt gaatcgccgt tgcagctcct taatctcgaa
     1321 gcgcatcgac tcccgaagct tggggtcgag attcgacagc ggctcgtcga gcagcagcac
     1381 cttcgggttc atgacgatgg cccgggcgag cgcgatccgc tggagctgcc ctccggacag
     1441 gtccgaggga aacgtccggt ccaaaccgcc gagtccggta tgtttgaggg cctccgcgac
     1501 cctggaatcc atctccctcc tcccgacgtt ccgcacccgc agagggaagg caacattttc
     1561 gaagacgttc agatgcggcc aaacggcgaa cgcctggaag accatcccca gcccccggtc
     1621 ctccggctgc acgtagagat tgcgcttctt cgcggacacc ggcttgtcgc cgatccagat
     1681 ctctccgtcg ctgagatcct cgaaccccgc gatcatccgg agcgtggtcg tctttccaca
     1741 tcccgacggc cccaacaggg agaagcactc gccctccctg atcgtcaaat tgaagtcgtc
     1801 gaccgcggtc acctctccca aagtcctcgt ggagaatttc ttcgtgacgt tcttcaggat
     1861 aacctcgctc atgagacgct cctcctgtcc ttggtaacaa accggagcgc caggttaccg
     1921 agcatgatga tcagtatggc gataccggac agcgccgccg actgggtgat attcccctcg
     1981 gcgttgatgg cgtagatcgc cacgcccagc gtgcgggaga acggcccgta gagcagaatc
     2041 gaggtggtca gctccctcat ggccggcaga aagatgagga aaaatcccgc gatcataccc
     2101 ggccgtatca gcgcgatggt gatgtcccgg agcgattcga ggtgcgtcgc gccgcaagag
     2161 cgggccgcct cctcaagaga gtagtgcacc tgctccagcg aggcggacga actcttcatc
     2221 gcgaacgcca cgtaccgcgc gatataggcg atcaggatga tccagatggt gttgtacagg
     2281 ttcagataga acgtgccgct ccacatcagg atcacgccga tggcgagcac gatccccggc
     2341 accgaatagg gaagcagcgc gaggatctcc agaagcgact tccccttgac cttcaccttt
     2401 accaccacgt aggcgatcat ggtgcccacg aacatcgcga taatcgcgga cgaaatggcc
     2461 agaaagaagc tgttcttgat agcgtcctgc gccatccgcg aggagaagag cacgtgatag
     2521 tagttatcca gcgtcatgtt ggcgagattg agcggcagcc cgtacgcctt cagccctccc
     2581 accgcgaaga tgatatagag cggcagaatg acgatcacga acaggctggc gaggcagacg
     2641 cccagcagcg ggaacttgga cgtccggagg ttgatcagca tgggtcgcat actcttgccg
     2701 cggataatat cgtagtgccc ggccttcaga atcttccgct gcagccagag tgcgatgacg
     2761 accaccacca cgagcagaat ggagagcgcc gccccctgcc ggattccctc gaagctcccg
     2821 gccgaccggt tgatcagctc atagatcttg gtgggaaggg tgaatatccc ccgagagaag
     2881 ccgagcatcg ccggaacgcc gaaatgcgac agcgaggaga tcagaatgag catcgcgccg
     2941 gccgcgacgg cgggcactac gagcgg
//
LOCUS       DAOTIL010000273         2962 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminobacteriaceae bacterium isolate AMR_MDS_3026
            NODE_39532_length_2962_cov_2.318541, whole genome shotgun sequence.
ACCESSION   DAOTIL010000273 DAOTIL010000000
VERSION     DAOTIL010000273.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868050
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminobacteriaceae bacterium (wastewater metagenome)
  ORGANISM  Aminobacteriaceae bacterium
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae.
REFERENCE   1  (bases 1 to 2962)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2962)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:19:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,224
            CDSs (total)                      :: 3,198
            Genes (coding)                    :: 3,184
            CDSs (with protein)               :: 3,184
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2962
                     /organism="Aminobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_39532_length_2962_cov_2.318541"
                     /isolate="AMR_MDS_3026"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:3034609"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            988..2439
                     /locus_tag="P5201_06625"
     CDS             988..2439
                     /locus_tag="P5201_06625"
                     /inference="COORDINATES: protein motif:HMM:NF012936.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004066 - asparagine synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006529 - asparagine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine synthase-related protein"
                     /protein_id="HRV98245.1"
                     /translation="MCMESAASLFGCAAPGKSLLPPGDTACFSRDRAGVEPLLYAGLG
                     GGIVSGKTYEELFAAGVPRKWRCEALVDFLATGTCVFPGGKTFFEGVFEVPPGHDLFV
                     SEGSASVVEREPLPEASGELGEETLPLLRDLLEEALGDGLETAALCLSGGLDSSSLAA
                     AWAKRGTPRCFVYGPPGAPDRERALEAAKSLGIDPVLLEPDGPAKLDELKELFRILEI
                     PVHIPGAPLPQFRLIRAMAGDGVKTVLSGQGGDELLCGYPWHFPLAMKKLRSGDPEAA
                     ARLEALHERNPPFGPIELRMTRRCFTRTSSWVVLNDGGACAALGLSHEEAAEREGVRF
                     FGADLDDWDALRRHGLTRRSLRYLLHYDYHLARHFRVEGRAPMLSADVVDLVSRFRLD
                     FLYGEGLLKYPLRLLFPELPESIRFEARKTGFWHNGPSLPDFRGEVRRMLETTILGDL
                     VIRPEMVEKMTPAALWRFFSAGTLLEENGRIGR"
     gene            2520..2783
                     /locus_tag="P5201_06630"
     CDS             2520..2783
                     /locus_tag="P5201_06630"
                     /inference="COORDINATES: protein motif:HMM:TIGR03798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Nif11-like leader peptide family RiPP precursor"
                     /protein_id="HRV98246.1"
                     /translation="MSMESAVAFYERVKKDVELQKKLKELGSKEKIQSYVKNDLGYDF
                     TKEEMQSVLFEQNPKMTDEDLEAAVGGVGYTQFDGIFIAAVYA"
BASE COUNT          644 a          778 c          893 g          647 t
ORIGIN      
        1 ccatcgctct gtactcgtct tcatctctgt cgggaaccac agggaacatg taaaaaatag
       61 ccaacagcac ggcgatacct gcaatggcaa aaggtaccgc ctgcctgaaa gaaaagttcc
      121 gcaaagtatg acgcaggact ttcatgagtg ctttcccctc cggagtttgg aattcggcat
      181 ttcggaaacg gacactcctt ggatagcgga aattttactc ctgttgccac ttcggcaggt
      241 tccccggcca gtgcagaaaa aagcttgtag atcaaccggc aaaataccgg caaacgaaat
      301 ttttgaataa taatcatagc acttttcctg atggaatcag cttgcgtgtc taaaaagcgc
      361 agaacaatcg atacgtgttt ttccatcctg gcagcaccgt cagcctctct ttccaaattt
      421 gagcgattcg gatgatcgtt gtgtttatga aagcgccttg tgacccttcc ggcacgatgg
      481 ggccagtcac ttttttccac gacggtcgat caaaaaaacg tgcagagcgc ctgtgttttt
      541 cctggaaatc gggtgaataa ttttggcctg gagcctcttt cctgaccggc ggaccacgga
      601 ctgctatctc gggaagggac cttctatccc atggtgcctc ctggaatttg tcgaataagt
      661 atccttttgt tacaatgagc ccgccccaac agtttgccga tagccacata tttctctttt
      721 ttgccgccca agacacgttt taagcagttc tccgaaacct tttcaaagct gctttcgcga
      781 gagttatatt caaaaaacgt cccttccatt ttgttttgga agagatcttc gatccgtttt
      841 ctgaaagaga gcatttcgag gaatatttct tgaccttgac acagtgtgaa cgtttagaat
      901 gaatccgaag ccgacgacac gggagaaccg gacagtttca ccgtccattc cgcgatggag
      961 gggtcgaatt caagggaggt gctgaggatg tgcatggaat cggcagcaag cttgttcggg
     1021 tgtgccgcgc cgggaaagag tcttcttcct cccggggaca cggcatgttt ttcccgcgac
     1081 cgcgcggggg tggagccttt gctctatgcg gggctgggcg gcggaattgt gtcgggaaaa
     1141 acctatgaag agctttttgc cgcaggcgtt cccaggaaat ggcgttgcga ggcacttgtc
     1201 gatttcctcg cgacgggaac gtgtgtcttc cccggcggca agacattctt cgagggagtc
     1261 ttcgaagttc cccccggaca tgacctgttc gtctcggaag gaagcgcctc ggtcgtcgaa
     1321 cgggagccgc ttcccgaggc atccggcgag ctgggtgagg agacgctccc cttgctgcgg
     1381 gacctgctgg aagaggctct tggtgatggg ttggaaacag ccgcgctctg cctgagcggc
     1441 ggattggact cgtcgtcttt agccgctgca tgggcaaaga ggggaactcc ccgctgcttc
     1501 gtctacggac cgccgggcgc acccgaccgg gagcgcgctc tggaggccgc gaaatctctg
     1561 ggcatcgatc cagtactgct tgagccggac ggccccgcga agctcgacga gctgaaggag
     1621 cttttccgga ttctcgaaat cccggtccac atccccggcg ctccccttcc gcagttccgc
     1681 ctgattcgcg cgatggcggg cgatggagtg aaaaccgtcc tcagcggtca gggaggagat
     1741 gaactcctct gcggctaccc ctggcacttc cctctcgcga tgaaaaagct gcgaagcggt
     1801 gaccccgagg ctgcggcacg gctcgaagcg ctccacgaga ggaatccccc cttcggtccc
     1861 atcgaactga ggatgacacg gcgctgcttc acccggacat ccagctgggt cgtcctgaac
     1921 gacggagggg cgtgcgctgc acttggcctc tcgcatgagg aggccgctga acgggaaggc
     1981 gtgcgcttct tcggcgcgga cctggacgac tgggacgcgc tgcgacggca cggcctgacc
     2041 cggaggagtc tgcgctacct tctgcactat gactaccatc tggcgcgcca ttttagggtc
     2101 gaggggcgag ctcccatgct gagcgcggac gtggtcgatc ttgtctcgcg cttccggctg
     2161 gactttctct acggagaggg attgctcaaa tacccgttgc gcctgctctt tccagagctt
     2221 ccggaaagta ttcgcttcga ggcccgcaag acggggttct ggcataacgg accttccctt
     2281 ccggattttc gtggcgaagt gcggcgcatg ctggagacga cgatcctcgg cgatctggtc
     2341 atccgtccgg aaatggtgga gaagatgacc cccgctgccc tttggcgctt cttctccgcg
     2401 gggacgctgc tggaggaaaa cgggcggata gggaggtgat tgtgacgcag agagaagaag
     2461 cggattgaaa cgaatagaag ctcttccaga aagaaagaga acatacggag gttttgtcta
     2521 tgtccatgga atcggcggtt gcgttctacg agcgggtgaa gaaggatgtg gagttgcaga
     2581 agaagctgaa ggagcttgga agcaaggaga agatccaatc atacgtaaaa aacgatctgg
     2641 gctacgattt caccaaagag gagatgcagt cggtgctctt cgagcaaaat cccaagatga
     2701 ccgacgaaga tctggaggcg gccgtcggag gagtcggtta tacgcaattc gacggcatat
     2761 tcatcgccgc tgtctacgcc tgactggata cagaggtgta tgggagagcg gggatattcc
     2821 tggaagggaa gtgaatggat gaaacgggaa gcggcaggag aggcgaagag agaacaaccg
     2881 ccggtcttgt tccggggcga agagcttcgg ttgcggaaga tcgccgacga ggaaggaatc
     2941 tctgcgcatc ccgtgggggc gg
//
LOCUS       DAOTIL010000274         2960 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminobacteriaceae bacterium isolate AMR_MDS_3026
            NODE_39561_length_2960_cov_3.556627, whole genome shotgun sequence.
ACCESSION   DAOTIL010000274 DAOTIL010000000
VERSION     DAOTIL010000274.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868050
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminobacteriaceae bacterium (wastewater metagenome)
  ORGANISM  Aminobacteriaceae bacterium
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae.
REFERENCE   1  (bases 1 to 2960)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2960)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:19:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,224
            CDSs (total)                      :: 3,198
            Genes (coding)                    :: 3,184
            CDSs (with protein)               :: 3,184
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2960
                     /organism="Aminobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_39561_length_2960_cov_3.556627"
                     /isolate="AMR_MDS_3026"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:3034609"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..382
                     /locus_tag="P5201_06635"
     CDS             <1..382
                     /locus_tag="P5201_06635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009163612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HRV98247.1"
                     /translation="ENKVMAAVGPTLTGKPKIELRNKTWDTPVILWKAGVHFCIITDH
                     PVVPIEHLSVCLSLAVRAGLPREEALRAVTLSAAEHLGIADRVGSIEKGKDADIVVWD
                     GDPLDARSRAVATIIDGETAYSRI"
     gene            complement(475..2115)
                     /locus_tag="P5201_06640"
     CDS             complement(475..2115)
                     /locus_tag="P5201_06640"
                     /inference="COORDINATES: protein motif:HMM:NF014871.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008253 - 5'-nucleotidase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-sugar
                     hydrolase/5'-nucleotidase"
                     /protein_id="HRV98248.1"
                     /translation="MKQRMRSTVRSVFLVCFLTVFLSAPEASGADGRVVIFALNDMHS
                     QLLPVTTKVKTTTVLFGGLAHAGGVIAEQRAAEKDPVLALQAGDMVAGLMWRLFSGEP
                     EFSALEHAGVQAGTLGNHEFDYGVEHLKKLLSFTSMPMVVSNLEFDDDFLKQRVLKRL
                     MLQAGAMKVGVFGLASPNLFALAGAGDSVKVDAALRQISEKMVAGLREEGADAILLLS
                     RLSDEENEALAAGVEGIHAILGGSSHNELHETRIVEGPNGWKTALGRAGAYCAFVGRL
                     RLAVKDGKLDEQETEWSLLRVTPRARTDGDVLALALEYERKLNEAMLFTIGTFSDAAD
                     ARAQTVRARESALGNLIADSLRWRMKTDVAVINGGGVRGDKIFPAGSVSRKVLAEILP
                     FDNEIHIVLLTGEQLRKAFEIAASSLVGKDDRYESSVRTYAGGFLQISGARVVYSLSG
                     RPTLVDPDGKVLEWGNRVKSLHVERDGAWMPVEDDSSYTLAANSWTATGGDRFSVLGN
                     APQQKTDFKDLDVLADYIASHPEGRVRLELDGRITVEE"
     gene            complement(2120..>2960)
                     /locus_tag="P5201_06645"
     CDS             complement(2120..>2960)
                     /locus_tag="P5201_06645"
                     /inference="COORDINATES: protein motif:HMM:NF012772.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HRV98249.1"
                     /translation="KNGLGRIAYCRAGDVTANGAGGENILTLQAELHAALDRDEFIPF
                     FQPVYSIASGRLSGFETLSRWKHPQKGILPPAAFIPQAEQTGFVANLDTRMLEHALDA
                     VNTARRLNPQLRLFFSSNASSVFLRSAAAVESVKFFLESTGADPTRFTLEVTESSLIE
                     NLDDVHVKLDLLKESGIRIALDDFGTGYSSLQYINNLPLDVIKLDRAFVSQLFVSAKT
                     ERMMRSIITMGRDLGLDIIAEGVETAEQLAWLKSAGCPKAQGYYFSPPVPWDEAQRMI
                     QEV"
BASE COUNT          542 a         1002 c          881 g          535 t
ORIGIN      
        1 cgaaaacaag gtgatggcgg ccgtaggccc cacgctcacc ggcaaaccga agatagagct
       61 ccgcaacaag acgtgggaca ccccggttat cctctggaag gcgggagtac acttctgcat
      121 catcacggac catccggtgg tccccatcga gcacctctcc gtctgcctct cgctggccgt
      181 ccgcgccgga ctgccgcgcg aggaagcgct gcgggccgtc acgctctccg ccgccgagca
      241 cctgggcatc gcggaccgcg tcggctcgat cgagaagggg aaggacgccg atatcgtcgt
      301 ctgggacgga gaccccctgg acgcgcgaag cagggccgtc gcgacgatca tcgacggcga
      361 gactgcctac tccagaatct gacgccggaa tccgacgaac ggggggcggc cgcgcctgcg
      421 cagaccgccc cccgttatca ttctctcccg gcctctccag aacgctcccc gccgctactc
      481 ctccacagtg atccttccgt ccagttcgag gcgcacccgc ccttccgggt gactggctat
      541 gtagtcggcg aggacgtcga gatccttgaa gtccgttttc tgctgcggcg cgttgccgag
      601 caccgagaat ctgtcgcctc cggtcgccgt ccacgaattc gcggcaagcg tgtacgagga
      661 gtcgtcctcg acgggcatcc atgccccgtc gcgctccaca tggaggctct tgacgcggtt
      721 tccccactcg agtaccttgc cgtcgggatc gaccagcgtc ggacggccgg agagcgagta
      781 gacgacgcgc gccccggaga tctggaggaa ccctcccgcg tacgtgcgaa cggaggactc
      841 gtatctgtcg tcctttccca cgagggagga cgcggcgatt tcaaaggcct tccggagctg
      901 ttcgcccgtc aggaggacaa tgtgaatctc gttgtcgaag gggagaatct ccgccagcac
      961 tttccgcgag acgcttcccg cagggaatat cttgtccccc ctgacgcccc cgccgttgat
     1021 gacggcgacg tcggtcttca tcctccaacg gagcgaatcg gcaatcaggt tgccgagggc
     1081 ggattccctg gcgcgcaccg tctgcgcgcg ggcgtccgcg gcgtcggaga aggtcccgat
     1141 ggtgaagagc atcgcctcgt tcagcttgcg ctcgtattca agcgcaagcg ccagcacgtc
     1201 gccgtcggtc cgggcgcgcg gcgtcacgcg gagcaggctc cattcggtct cttgttcgtc
     1261 gagcttcccg tccttgacgg cgagccggag cctgcccacg aaggcgcagt aagcgcccgc
     1321 gcgtccgagc gccgtcttcc agccgttcgg cccctcgaca atgcgcgtct cgtgcagctc
     1381 gttgtgggag gagcccccca gtattgcgtg aatcccctcc accccggcgg ccagcgcctc
     1441 gttctcctcg tcggagaggc ggctcagcaa cagaatggcg tccgccccct cttcccggag
     1501 cccggcgacc atcttttcgg agatctgccg cagcgctgcg tccaccttca cggaatcgcc
     1561 cgcgcccgcc agagcgaaga gattcggcga agccagtccg aaaacgccca ccttcatggc
     1621 gcccgcctga agcatgagtc gtttcaaaac ccgctgtttc aaaaagtcgt cgtcgaactc
     1681 caggttggag acgaccatcg gcatggaggt aaacgagagc agttttttga gatgctccac
     1741 gccgtaatcg aactcgtgat tgcccaaagt ccccgcctgc acgcccgcgt gctccagcgc
     1801 cgagaactcc ggctccccgg agaagagccg ccacataagg ccggcaacca tatccccggc
     1861 ctgaagcgca agcaccgggt ctttctcggc ggcgcgctgc tccgcgatga cgccgcccgc
     1921 atgggcaagc cctccgaaga gcaccgtggt cgtcttcact tttgtggtca cgggaagaag
     1981 ctggctatgc atatcgttca acgcaaaaat aacaacccgc ccgtcggctc cagacgcctc
     2041 gggggcggag agaaacaccg tcaggaagca tacaagaaaa actgaccgta ccgtactccg
     2101 catcctctgc ttcattgcct cacacctcct gtatcatccg ttgcgcctcg tcccacggga
     2161 cgggcggaga gaagtagtac ccctgcgctt tcgggcaccc cgcgctcttc agccatgcga
     2221 gctgctccgc cgtctccacg ccctccgcga taatgtcgag ccctagatct cttcccatgg
     2281 tgatgatcga gcgcatcatc cgctccgtct tcgcggaaac gaaaagttgg ctgacaaacg
     2341 ccctgtccag cttgatcacg tcgagcggca ggttgttgat atattggagc gaggagtagc
     2401 ccgttccgaa gtcgtcgagg gcgattctga ttccgctctc cttcagaaga tcgagtttga
     2461 cgtgcacatc gtccaggttt tcgatcagcg aactctccgt cacctcaagg gtgaagcgcg
     2521 tcggatcggc ccctgtgctc tccaggaaaa acttcacgct ctccacggcg gcggcgctcc
     2581 tcaggaagac cgacgaggcg ttcgacgaga agaagagccg aagctgcggg ttcagcctgc
     2641 gcgccgtgtt gaccgcatca agagcgtgct ccagcatgcg ggtgtcgagg tttgcgacga
     2701 atccggtctg ctcggcctgc ggtataaagg ccgcgggagg aaggatcccc ttctgcggat
     2761 gcttccaacg ggagagggtc tcgaaaccgg agagcctccc ggacgcaatg ctgtagacag
     2821 gctggaagaa gggaataaac tcgtccctgt ccagagcggc atgcagctcc gcctgcaagg
     2881 tgaggatgtt ttcgccgcct gctccgttgg ccgtcacatc ccccgcccgg cagtacgcta
     2941 tccggcccag cccgtttttc
//
LOCUS       DAOTIL010000275         2956 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminobacteriaceae bacterium isolate AMR_MDS_3026
            NODE_39624_length_2956_cov_4.827990, whole genome shotgun sequence.
ACCESSION   DAOTIL010000275 DAOTIL010000000
VERSION     DAOTIL010000275.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868050
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminobacteriaceae bacterium (wastewater metagenome)
  ORGANISM  Aminobacteriaceae bacterium
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae.
REFERENCE   1  (bases 1 to 2956)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2956)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:19:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,224
            CDSs (total)                      :: 3,198
            Genes (coding)                    :: 3,184
            CDSs (with protein)               :: 3,184
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2956
                     /organism="Aminobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_39624_length_2956_cov_4.827990"
                     /isolate="AMR_MDS_3026"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:3034609"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..309)
                     /locus_tag="P5201_06650"
     CDS             complement(<1..309)
                     /locus_tag="P5201_06650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014805969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HRV98250.1"
                     /translation="MKRPIAFALVLTIVCFLSGSAWSANGKFNAESYSSPKIRDLYLV
                     IIRDPDAQLLALQRGQADILGDLHRPVDVDTLADDPRVNLSLASGFHGFFLGFNVRTF
                     P"
     gene            complement(464..1270)
                     /gene="arsM"
                     /locus_tag="P5201_06655"
     CDS             complement(464..1270)
                     /gene="arsM"
                     /locus_tag="P5201_06655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012869251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arsenite methyltransferase"
                     /protein_id="HRV98251.1"
                     /translation="MDDIRNYVKERYAQAIKNKSSCCGSGGCCCGTTGASGIPSMTRG
                     NYDGATLEKTPEQMADQSFGCGNPFTEARIHPGETVLDLGSGAGLDLFLASEMTGPYG
                     KVIGLDMTDEMLATAAENLKGIENVSLVKGYIEEMPVESNSVDVIISNCVINLSPDKG
                     KVLKEAFRVLRPGGRFCVSDTVFLRPVTERAVKNLAAWSGCISGALRETEYAEKMREE
                     GFEEVEVRRTKVYSFPDAMAAMAFPELSGEERNEINGSLASAIIMGKKPL"
     gene            complement(1278..1733)
                     /locus_tag="P5201_06660"
     CDS             complement(1278..1733)
                     /locus_tag="P5201_06660"
                     /EC_number="1.20.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014163905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030612 - arsenate reductase (thioredoxin)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arsenate reductase ArsC"
                     /protein_id="HRV98252.1"
                     /translation="MSKKPKVLFLCTGNSCRSQMAEGWTRHFHSAAIEAYSAGIETHG
                     LNPFAVEAMKEAGADISGQRSKNVMELLDVSFDYVVTVCGDADERRPFFPGQTRVLHV
                     GFDDPPKLALLEETEEGKMNCYRRVRDEIRAFIKDLPGFLENLPEEKRS"
     gene            complement(1740..2105)
                     /locus_tag="P5201_06665"
     CDS             complement(1740..2105)
                     /locus_tag="P5201_06665"
                     /inference="COORDINATES: protein motif:HMM:NF033788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HRV98253.1"
                     /translation="MESILSISKALGEKNRLRVFLALMHTKELCVCEVGELLGLAPAT
                     VSRHMSILSRAKLVDSRKRGKWVYYCIKPAIAPELLQWIKNSVVDSFSVAEDVKRIEI
                     LVRERQDKDCTKACVSEER"
BASE COUNT          608 a          829 c          811 g          708 t
ORIGIN      
        1 cgggaaggtg cggacgttga acccgaggaa gaagccgtga aaaccggatg cgagcgagag
       61 gttcaccctc ggatcgtcgg cgagcgtatc gacgtcgacg gggcggtgca gatctccgag
      121 gatatccgcc tgccctcgct gaagcgccag aagctgggcg tcgggatccc tgatgatcac
      181 caggtaaaga tcccggattt tcggagagga gtagctctcc gcgttgaact ttccgtttgc
      241 gctccatgcg cttccggaaa gaaaacagac gattgtaagg acaagtgcga aggcgatcgg
      301 tctcttcatc tctttcccat ccttcccgta tccctgagga gtatgttttc aagtatttta
      361 ccagaagacg cggcgaaaca acgtctgaaa aaaagtgccg gaaccagaag agggagtccc
      421 ccggtaccgg cgctttctga tttccgcgaa tctgacgaac gccctaaagc ggctttttcc
      481 ccatgatgat cgcgctggcc aacgagccgt tgatctcgtt tcgttcttcg ccggagagtt
      541 cggggaaggc catcgccgcc attgcgtcgg ggaaggagta caccttcgtg cgccgtactt
      601 cgacctcctc gaagccctcc tccctcatct tctccgcgta ctccgtctcc cggagcgctc
      661 cggaaatgca cccggaccac gccgccaggt tcttgacggc gcgctccgtc acaggacgga
      721 ggaagacggt gtcggagacg cagaacctcc cgccgggacg gagaacgcgg aaagcctcct
      781 tcagcacctt tcccttgtcc ggggaaaggt tgatcacgca gtttgaaatg atgacatcga
      841 cggagttgct ctcgaccggc atctcctcga tgtacccttt caccagggag acgttttcga
      901 tccccttgag attttcggcg gcggtcgcaa gcatctcgtc cgtcatgtcg agcccgatga
      961 ccttgccgta cgggccggtc atctccgacg ccagaaagag gtcgagcccc gcgccgcttc
     1021 ccaggtcgag gacggtctcg cccggatgga tgcgcgcctc ggtgaacgga ttgccgcagc
     1081 cgaaggactg gtcggccatc tgctccggag ttttttcgag cgtcgcgccg tcgtagttgc
     1141 cgcgcgtcat gctcgggatg ccggatgcgc ccgtcgttcc gcagcagcac cctcccgagc
     1201 cgcaacacga ggacttgttc ttgatcgcct gcgcgtaccg ctctttcacg tagttgcgaa
     1261 tatcgtccat acttgatcta gctccttttt tcctccggta gattttcaag aaagccggga
     1321 aggtctttga tgaatgcgcg tatctcgtcg cgcacccgcc ggtagcagtt catttttccc
     1381 tcctccgtct cttcgaggag cgccagcttc ggcgggtcgt cgaagccgac gtggaggact
     1441 ctcgtctgcc ccggaaagaa cgggcgccgc tcgtccgcgt cgccgcacac ggtgacgacg
     1501 tagtcgaatg aaacgtcgag cagctccatc acgttcttgg agcgctgtcc ggagatgtcc
     1561 gctcccgcct ccttcatagc ctccacggcg aacggattca ggccgtgcgt ctcgattccg
     1621 gcagaatacg cttctatggc ggcgctatgg aaatgacggg tccacccctc ggccatttgg
     1681 ctgcggcacg agttcccggt gcagagaaag agcacctttg gctttttgct cacgttattt
     1741 cacctctctt cggatacaca ggcttttgta cagtctttgt cctggcgttc cctgacgagg
     1801 atctcgattc tcttcacgtc ttccgcaacg gaaaaagaat cgacgacgga attttttatc
     1861 cattggagta actccggcgc tatcgcaggc ttgatacagt aatagaccca tttgcctcgt
     1921 ttacgggagt cgacgagctt tgcccgggag agaatgctca tgtggcgcga cactgtcgcg
     1981 ggggcaaggc cgagaagttc ccccacctcg cagacgcaaa gttccttcgt gtgcataagc
     2041 gccagaaaga cgcggagcct gttcttttct ccgagcgctt ttgaaatgga aagtatggat
     2101 tccatagctg actcctttat ttcgttaatt ttacatatga cgaaatgata agcgggggcg
     2161 ccctctttgt caagaggctt cgtcgggtag tttcttttcc tgggcgtaag cgctcgtcaa
     2221 atcgaagaga catcatgcat tttgtacaag ttagaggcgt atccgcacat acttacatta
     2281 aaatgtatac cgtaaattat gttagctcat ttacaatttc ctttttttta tttcactcgt
     2341 tgacaatgtg gcgaaaaatg gtatatcatc gcacgggtga agcagcaaca tacttccgaa
     2401 gattgcgcga gaatcagaag accgtgtcgg ctccggtaac acaacccaat cctttcgcgg
     2461 catttcgtcg cttcaagcgg tggtgtgcgg caaggagcga tttccccgga gccctgccgg
     2521 gacaaataag gaatggcgaa gcgtagtgga cgccgtggat atctttcgtt agaaaggtat
     2581 tatcatcttc accagcgcga catgatttat gattttcccc ttcgccgccg acatgctccg
     2641 gtaaagtctc cctattaagg ttttcacgaa tccccttaac catcgggaga tttgtgagcg
     2701 tacgtccgtt gattctttgc tctatctgcc ctgaggttcc tgcggaaccg tatgtgagga
     2761 gggatgccaa gcattgagta gggatgtaag agtgcgtttc tgagccaccg ttctttgttc
     2821 ttatcttacg aaggaggtat gaaatgcaat gacgaatctt ctgggtgaat tcatcggaac
     2881 catggtactg gttactttcg gcgacggtct cgtcgcctgc gcgctgctca acaagtccaa
     2941 gggacagaac gcggga
//
LOCUS       DAOTIL010000276         2954 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminobacteriaceae bacterium isolate AMR_MDS_3026
            NODE_39678_length_2954_cov_4.108313, whole genome shotgun sequence.
ACCESSION   DAOTIL010000276 DAOTIL010000000
VERSION     DAOTIL010000276.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868050
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminobacteriaceae bacterium (wastewater metagenome)
  ORGANISM  Aminobacteriaceae bacterium
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae.
REFERENCE   1  (bases 1 to 2954)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2954)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:19:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,224
            CDSs (total)                      :: 3,198
            Genes (coding)                    :: 3,184
            CDSs (with protein)               :: 3,184
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2954
                     /organism="Aminobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_39678_length_2954_cov_4.108313"
                     /isolate="AMR_MDS_3026"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:3034609"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            115..1296
                     /locus_tag="P5201_06670"
     CDS             115..1296
                     /locus_tag="P5201_06670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013048368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAM domain-containing protein"
                     /protein_id="HRV98254.1"
                     /translation="MTEGFGKIVQLIFSGLMILIGGMIGAQVAQLLFPIIPAEWFAAW
                     KEWLPLWMPGKILMTIFSVVLFAFIGLMTSPLFLRVLGSMGSFIESHLKNTSWSEITS
                     ATVGMIIGLLIANLLALPFSDLPFGSYLAVVLNVVLAYLGARILLKRQSDLRNVFGPI
                     RSLKERLSMIKEHPHHDDEGFPAEEQPWEVPKKILDTSVIIDGRILDVARTGFLEGII
                     LIPQFVLHELQAVADSTDPARRTRGRRGLDVVNELQRLSDLQIEIVDASLKHLKADSV
                     DSALVVLAEKIGGQILTTDYNLNKLAQIQGIRVLNVNDLANALKPMLLPGETIIIDII
                     REGKEPHQGVGYLDDGTMFVVEDGENYIGKRVEVVVTSMLQTSAGRMVFGRIRREVRA
                     S"
     gene            1293..2459
                     /gene="ispF"
                     /locus_tag="P5201_06675"
     CDS             1293..2459
                     /gene="ispF"
                     /locus_tag="P5201_06675"
                     /EC_number="4.6.1.12"
                     /inference="COORDINATES: protein motif:HMM:NF013308.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008685 - 2-C-methyl-D-erythritol
                     2,4-cyclodiphosphate synthase activity [Evidence IEA];
                     GO_process: GO:0019288 - isopentenyl diphosphate
                     biosynthetic process, methylerythritol 4-phosphate pathway
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-C-methyl-D-erythritol 2,4-cyclodiphosphate
                     synthase"
                     /protein_id="HRV98255.1"
                     /translation="MKEASLVIVAAGKGERAGGAGGTAKQFRPLAGIPLWKWSASAGE
                     RLFNKNVLHECILVVPESAVESVQRETLGWKIPFFVVPGGAERQESVVRGIRAASREY
                     VLVHDGARPFLSGALVERLLESLTDEYGVAPLLPVSDALKKTGEDGAFSPFPREGLWV
                     TQTPQAFHREKLLSVLQTHGGGAKDEAEAWIAAGHPLRSVRGERKNIKITWEEDFSLA
                     ELYVQRTFRTGTGYDVHPLVPGRRFILGGVEFPEFPLGFAGYSDGDVLAHAVCDALLG
                     AAGLGDIGTLYPAGNPAFKNISSLLLLSDTAKRVTDAGWILEWSDSVICAQEPKLAAS
                     LSRMVSKMEEVLPETWRGRVHLKVKSGEHVGPVGHSHCIVCYSNATLWKPLDII"
     gene            2572..>2954
                     /locus_tag="P5201_06680"
     CDS             2572..>2954
                     /locus_tag="P5201_06680"
                     /EC_number="1.1.1.205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008520278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IMP dehydrogenase"
                     /protein_id="HRV98256.1"
                     /translation="MSLESKFVPYEGFTFDDVLLEPAFSEVLPSDVSIKTNLTESIAL
                     NIPLCSSAMDTVTEARLAIAMAREGGIGIIHRNMPLEKQAKEVDVVKRSESGVIVDPF
                     FLHPEDMVREAISLMEHYHISGVPIV"
BASE COUNT          641 a          742 c          835 g          736 t
ORIGIN      
        1 atggattccc gtgtgcgagt caggcgatct tcctctattg tgccttagaa aaggagacgt
       61 gctgtgaaaa agtttaggct gatgacatat ttgtcaaaaa agcgaggtga aagtatgacg
      121 gaaggtttcg ggaaaatcgt ccagttgatt ttctcaggac ttatgatact tattggcggc
      181 atgatcggcg cccaggtagc tcagttactt ttccccatta ttcccgccga atggtttgcc
      241 gcctggaaag agtggctccc cctttggatg cccggcaaga tactgatgac aattttttct
      301 gtggttttat tcgcttttat tggtttgatg acctctcccc ttttcctccg ggttcttggt
      361 tctatgggct ccttcatcga atcgcatctc aaaaatacga gctggtcgga aattacttca
      421 gctaccgtcg gtatgatcat cgggttgctc atcgcgaatc ttctggctct tccattctcc
      481 gatctcccat tcggtagtta tttggctgtt gtgttgaacg tcgttttggc ctatcttggg
      541 gctcgaatac tactgaagcg gcaaagcgat ctccggaacg tattcggtcc aattcgaagc
      601 ctgaaagaac gtctttccat gatcaaggag caccctcacc atgatgatga aggttttccc
      661 gcggaagaac agccctggga ggttcccaaa aaaattctgg atacgagcgt tatcatcgac
      721 gggcgtattc ttgatgtcgc acgcaccggc ttcttggagg ggattatact tataccccag
      781 tttgttcttc acgaattgca ggccgtggct gattccaccg accccgcgcg aagaacgagg
      841 gggcgaagag ggcttgacgt cgtcaacgag ttgcagcgcc tttccgatct gcagattgaa
      901 atcgtcgacg cttcattgaa gcatctgaaa gcggattccg ttgacagcgc gctggtcgtt
      961 ttagccgaga agataggcgg acagattctt acaaccgatt acaacctcaa taaactcgcg
     1021 cagatccagg ggattcgtgt gttgaacgtc aacgatctgg caaatgcgct caaaccgatg
     1081 ctgcttcccg gagagaccat cataattgat attatacgag agggaaagga acctcatcag
     1141 ggagtcggct acctcgacga cgggacgatg ttcgtggtcg aggatggaga gaactatatc
     1201 ggcaaacggg ttgaggttgt cgttacctcc atgctccaga catccgccgg ccggatggtg
     1261 tttggacgga tacgaaggga agttcgcgcg tcgtgaaaga agcttctctt gtcatcgttg
     1321 cggcgggcaa gggagagcgt gccggaggag cgggcggaac agcgaaacag ttccgtccgc
     1381 tcgccggtat tcccctatgg aagtggagcg cttccgccgg agaacgcctc ttcaacaaaa
     1441 acgtactcca cgaatgtatt ctcgtcgttc cggagagcgc cgtagagtcg gtgcagaggg
     1501 agacgctcgg gtggaaaatc ccgtttttcg ttgtgccggg aggagccgaa cggcaggaat
     1561 ccgtcgtgcg cggtatccgc gccgcttccc gggaatacgt gctcgtccac gacggcgcgc
     1621 ggccttttct ctccggcgct ctcgtggaaa ggctccttga gtctcttacg gacgagtacg
     1681 gagtggcgcc cctgcttcca gtatccgatg ccctgaaaaa gacgggagag gacggggctt
     1741 tttcgccctt tccgagagag gggctctggg ttacgcagac tccgcaggcc tttcaccggg
     1801 agaaattgct ctccgtgctc caaactcatg gaggcggagc caaggacgag gcggaggcat
     1861 ggatcgccgc aggccatcct ctccgttcgg tcaggggaga gcgcaaaaat ataaagataa
     1921 catgggagga ggacttctct ctggcggaac tctatgtgca gcgaactttc cgtaccggaa
     1981 ccgggtatga tgtgcaccct ctcgttcccg gcagaaggtt cattctcggc ggtgtcgaat
     2041 tcccggagtt tcctctcggt ttcgctgggt actcggacgg ggacgttctg gcgcatgccg
     2101 tgtgcgacgc gcttctgggg gccgcagggt tgggtgatat cggcacgctc tatccggcgg
     2161 ggaatcccgc gttcaaaaac atctcgagtc tccttctgct ttcggatact gcgaaaagag
     2221 tcacggacgc aggatggatc cttgagtgga gcgacagtgt catttgcgct caggagccga
     2281 agctcgccgc ttctctctcc cgaatggtct cgaagatgga agaagttctc cctgaaacgt
     2341 ggcggggcag agtgcacctc aaagtgaaat ccggggaaca cgtaggaccg gtcggacatt
     2401 ctcactgcat cgtctgttac tcaaacgcga cgctctggaa acctcttgac attatttgag
     2461 ccggacgttt ttttgttgtt tttttcgtaa ggggagttgc ttctttttcc ggaaagccct
     2521 acaatccttt aaaatctttt acgagtccct aacgggcagg gaggtaccgt aatgagttta
     2581 gagtccaagt tcgtccccta cgagggattc acatttgatg acgttcttct cgaaccggcg
     2641 ttcagtgagg ttttgccgtc cgatgtttct atcaagacaa atctgaccga atcaattgct
     2701 ctgaatattc ctctatgcag ttccgcgatg gatactgtga cggaggctcg tttagccatt
     2761 gcaatggccc gagagggcgg gataggcatt atccacagga acatgccgct ggagaaacag
     2821 gccaaggaag tggacgttgt caagcgttct gaatccggcg tcatcgtcga tccgtttttt
     2881 cttcaccccg aggacatggt gagggaagca atctctctta tggaacacta tcacatttcc
     2941 ggcgttccaa tagt
//
LOCUS       DAOTIL010000277         2951 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminobacteriaceae bacterium isolate AMR_MDS_3026
            NODE_39745_length_2951_cov_2.802486, whole genome shotgun sequence.
ACCESSION   DAOTIL010000277 DAOTIL010000000
VERSION     DAOTIL010000277.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868050
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminobacteriaceae bacterium (wastewater metagenome)
  ORGANISM  Aminobacteriaceae bacterium
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae.
REFERENCE   1  (bases 1 to 2951)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2951)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:19:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,224
            CDSs (total)                      :: 3,198
            Genes (coding)                    :: 3,184
            CDSs (with protein)               :: 3,184
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2951
                     /organism="Aminobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_39745_length_2951_cov_2.802486"
                     /isolate="AMR_MDS_3026"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:3034609"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..441)
                     /locus_tag="P5201_06685"
     CDS             complement(<1..441)
                     /locus_tag="P5201_06685"
                     /inference="COORDINATES: protein motif:HMM:NF025054.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HRV98257.1"
                     /translation="MEHVIEECGDPRSIPRDLLYLADPSDEMLNAYVDVSYAFVCRID
                     REIVGALLMMPTRPFTMEIMNISVREPYWNQGIGRKLIQGAVKKARETGMKKVEIGTG
                     NPGVVQMLLYQKCGFRITGVEFDYFRRCCAEKIIENGIECRDMIR"
     gene            complement(505..1827)
                     /locus_tag="P5201_06690"
     CDS             complement(505..1827)
                     /locus_tag="P5201_06690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008709709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HRV98258.1"
                     /translation="MYRAAIEKLKRWKAKEAPKPLIIRGARQVGKTWLMKEFGRSAYE
                     NTVYIGFENNRQMKELFSGDMAIDRIVTGLELYAGRKIDPENTLLVFDEIQEVPAALT
                     ALKYFNENAPQYRIVCAGSLLGVALHPGTSFPVGKVEFLDLFPLSFSEFMTAMGKERF
                     AELLQKGDFELATTFKDEFIDLLKHYYTIGGMPEAVASFAGNRDFNEAREIQRRILAA
                     YEQDFSKHAPNEIVPRIRMLWNSVPAQLTKENKKFVYGVVKEGARAKEYEMAMLWLAD
                     CGLVYKVHRATAPRLPLKAYEDLKAFKLFLLDVGLLSCMVRLDQRLLLEGSALFTEFK
                     GALTEQYVLQQLKTIEDVDTCYWTNERGGAEIDFLVDAGDEVVPVEVKAETNLKAKSL
                     KTFCEKFRPKSAVRVSMADYKREERLLNLPLYAVETIGNGGVLPEGGK"
     gene            complement(2020..2436)
                     /locus_tag="P5201_06695"
     CDS             complement(2020..2436)
                     /locus_tag="P5201_06695"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRV98259.1"
                     /translation="MESHSPRFWELFFEVFEDLPRQGPGSKTCAAEALALCRELPESP
                     SILDLGCGVGGQTLYLAALRDCGFEPVGHFPLPDEAWWDDFYTPMEARIAELRIKYAD
                     DQEASALLDQIAGEPEMHRRHSGYYAYEFFVARRPE"
     gene            complement(2476..>2951)
                     /locus_tag="P5201_06700"
     CDS             complement(2476..>2951)
                     /locus_tag="P5201_06700"
                     /inference="COORDINATES: protein motif:HMM:NF014609.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="PhzF family phenazine biosynthesis protein"
                     /protein_id="HRV98260.1"
                     /translation="RASIAEALGIREEAISDMLPVDIIDAGLRTLLVPIRALEQEVSM
                     YPDEQALKSYCLRNDVDIVLVFSMETAEPGYFAHTRVFAPKFGYLEDPATGSGNSAFG
                     YYLLKNKKWSGDAVRIEQGGDNRIFNEVKLMTTDGKVLFGGKAALKIEGNYFVGS"
BASE COUNT          552 a          908 c          800 g          691 t
ORIGIN      
        1 cctgatcata tcccggcact ctatcccgtt ttcgataatc ttttccgcac agcaccttct
       61 gaagtagtcg aattcgacgc ccgtaattcg aaagccgcac ttctggtaga gcaacatctg
      121 gacgacgccg ggatttcccg tccctatctc gactttcttc atgcccgtct cgcgcgcttt
      181 cttcacggcc ccttgaatca gctttcttcc tatcccttga ttccagtacg gctcgcgcac
      241 cgatatattc atgatctcca tcgtgaacgg gcgcgtcggc atcatcagca gcgcgccgac
      301 gatctcgcga tcgattctgc acacgaacgc gtaacttacg tccacatagg cgttcagcat
      361 ttcatcggac gggtcggcga gatagagcag atcccgcgga atgctccggg gatcgccgca
      421 ctcttcgatt acgtgctcca ctttcgatac acctctcttt catacatggt cgagccactt
      481 cgatccggcc cgacaaccgc aattctactt cccaccttcc gggagcacac caccgtttcc
      541 gatcgtttcc acggcgtaga gtggcagatt gagcagccgc tcctctcttt tgtaatcggc
      601 catggaaacg cggacggcgc tcttcgggcg gaatttttca caaaaggtct tcaagctttt
      661 tgccttgagg ttcgtctcag ccttgacttc tacggggacg acttcgtcgc cggcgtccac
      721 gaggaagtcg atctccgcgc cgcctcgttc gttggtccag tagcatgtat ccacatcttc
      781 gatcgttttc aactgctgca gaacgtactg ttcggtcaac gcgcccttga actccgtgaa
      841 aagagcgctg ccttccaaca gtagccgttg atcaagtcgt accatgcacg aaagaaggcc
      901 gacgtcgagc agaaaaagct tgaacgcctt caggtcttcg taggctttca gcggaaggcg
      961 gggggccgtc gcacgatgaa ccttgtagac cagtccgcag tcggcgagcc agagcatggc
     1021 catttcgtac tccttggccc tcgctccctc cttgacgaca ccgtagacga attttttgtt
     1081 ctccttcgtc agttgcgccg ggacgctgtt ccagagcatg cggattcggg gcacgatttc
     1141 attcggcgcg tgcttcgaga aatcctgttc ataggcggcc agaatgcgcc gctgtatttc
     1201 gcgtgcctcg ttgaagtcgc gatttccggc gaaactcgct acggcttcgg gcattccccc
     1261 gatggtgtaa tagtgcttca gcaggtcgat aaactcgtcc ttgaaggtgg tggccagctc
     1321 gaagtcgccc ttttgcagaa gctccgcgaa tcgctccttc cccatcgccg tcataaactc
     1381 gctgaaagag agcggaaaga ggtcgagaaa ctccaccttt ccgacgggaa aggacgtccc
     1441 cggatgcaac gcgacgccga gcagcgagcc cgcgcagacg attctgtact gcggggcgtt
     1501 ttcgttgaag tacttgaggg cggtcagggc tgcgggcact tcctgaatct cgtcgaagac
     1561 gagcagcgta ttctccggat cgatctttcg tccggcgtac agctccagcc ccgtgacgat
     1621 acggtcgatc gccatatcgc ccgagaacag ctctttcatc tggcggttgt tttcgaatcc
     1681 gatgtagact gtgttttcgt aggcgctcct tccaaactcc ttcatcagcc aggtcttgcc
     1741 gacctgacgc gctcccctga tgatgagcgg tttcggcgct tctttcgcct tccatcgttt
     1801 cagcttttcg atcgcggcac ggtacataac catcacctcg cgattgtgat tatacacccg
     1861 acgcacatat tttacgacga atacacggga atataacaca tattttacgg tgaaaaccga
     1921 agcgacgaaa gggctattcc actacgggag ctctcttctc ttcaagcgtt tccattcgtt
     1981 cgttcacgcg cgaacctcct cggttttctt cgcgaggccc tactcggggc ggcgggcgac
     2041 gaaaaattcg taggcgtaat agccggagtg gcggcggtgc atctctggct ctccggcgat
     2101 ttggtcgagg agggcggagg cttcctgatc gtctgcgtat tttattcgca gttcggcgat
     2161 gcgcgcttcc atcggcgtgt agaagtcgtc ccaccacgct tcgtcgggga gggggaagtg
     2221 tccgaccggc tcgaatccgc agtcgcgcag cgcggcgagg tacagcgtct gcccgccgac
     2281 gccgcacccc aggtcgagga tcgaggggga ttccggcagt tcgcggcaga gggcgagggc
     2341 ttcggcggcg catgttttgc tgccgggccc ctgacgcggc aggtcttcga agacttcaaa
     2401 gaagagctcc cagaagcgcg gtgagtggct ctccatgatc attctcctcc tcgttccgcc
     2461 ccggctccag ggcgcttagc tgccgacaaa atagttccct tcgattttca gcgcggcttt
     2521 tccgccgaac aggacctttc cgtccgtcgt catcagcttg acttcgttga aaattctgtt
     2581 gtcgccgccc tgctctattc tgacggcgtc cccgctccac ttcttgttct tgagcaggta
     2641 gtagccgaac gcgctgttcc ccgaccctgt ggcggggtcc tccaggtagc cgaacttcgg
     2701 ggcgaacact cgggtgtggg cgaagtaccc gggctccgcc gtctccatcg aaaagaccag
     2761 cacgatatcc acgtcgttgc gcaggcagta ggatttcaga gcctgctcgt cggggtacat
     2821 cgacacctcc tgctccagag cgcgtatcgg cacgagcagc gtccgaagcc ccgcgtcgat
     2881 gatatcgacg ggcagcatat ccgaaatggc ctcttcacgg atgcccaggg cttcggcgat
     2941 cgacgcccgc g
//
LOCUS       DAOTIL010000278         2951 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminobacteriaceae bacterium isolate AMR_MDS_3026
            NODE_39747_length_2951_cov_2.774517, whole genome shotgun sequence.
ACCESSION   DAOTIL010000278 DAOTIL010000000
VERSION     DAOTIL010000278.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868050
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminobacteriaceae bacterium (wastewater metagenome)
  ORGANISM  Aminobacteriaceae bacterium
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae.
REFERENCE   1  (bases 1 to 2951)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2951)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:19:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,224
            CDSs (total)                      :: 3,198
            Genes (coding)                    :: 3,184
            CDSs (with protein)               :: 3,184
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2951
                     /organism="Aminobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_39747_length_2951_cov_2.774517"
                     /isolate="AMR_MDS_3026"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:3034609"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            126..1736
                     /locus_tag="P5201_06705"
     CDS             126..1736
                     /locus_tag="P5201_06705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013047453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005436 - sodium:phosphate symporter
                     activity [Evidence IEA];
                     GO_process: GO:0044341 - sodium-dependent phosphate
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Na/Pi cotransporter family protein"
                     /protein_id="HRV98261.1"
                     /translation="MTWSSAFQILGGVGLFLFGIKLMSEALQYLAGDKMRQLIGSLTR
                     TPIRGVFIGALVAMLIQSSSGTTVMTVSFVHAGLMTLKQAVGVIMGANIGTTVIAQIV
                     AFKVKDFALPILGIGVLLVLFGRTKRQKYIGNGLVGFGLLFLGMQTMEGSMSFLRDRK
                     DLFIAFSSSPMLGLFVGTAVTMVVQASSATIGLTMAMAAQGLLPLEAALPILLGDNIG
                     TTITAVLASLGSNRAAKQAATAHVLFNVIGAALFLAFLPFYKDVVAATSTDIGRQIAN
                     AHTIFNVTNTLLFLPFVPVLVWVIRRIVPDKGGAVVAGPMYLDLKLISASPAAAVDAV
                     KKELLHMGDLARSMLTDVRRAFTENNPKMIGEVTQTEKAVNEINRAISSYASEVWQGG
                     LSSELSTVLGSYVNGVGDIERIGDHATNLIELYEYKLDHGVEFSTLARGEFDDMFDLV
                     ERAVRLSFESLNEEDVIKAKEVDALEDEVDRKEKILRKNHILRLNKGECTPAGGVIFI
                     DILSNLERVCDHAHNISLIAFDISKLHR"
     gene            1931..>2951
                     /locus_tag="P5201_06710"
     CDS             1931..>2951
                     /locus_tag="P5201_06710"
                     /inference="COORDINATES: protein motif:HMM:NF016203.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UxaA family hydrolase"
                     /protein_id="HRV98262.1"
                     /translation="MECTHDGARCIVLRENDSVAVLPEGGGAGSRLPFAEQELVLKEV
                     IAPGHKIALRDIKQGERVVKYGFPIGVASRPIAAGEHVHVQNVRSALGHEWSPSWSPS
                     PARLLSQLSPVPSFQGYRRTEGPPGIRNELWIIPTVGCVNEYLKFLAGAWTAPSWIEN
                     VRVLSHPYGCSQLGEDLERTAAVLAGLARNPCAAGVIIAGLGCENLQRVFLETRLAGV
                     EKVRFLSLQEEDDDAGALYALLDELAADAPKEREPFPLSGVTLGVKCGGSDGFSGLSA
                     NPLVGEVADRMSAWGGKILATEIPEMFGAEDVIASRIADRQVFDAFAETIRWFRNYYV
                     EHKQPV"
BASE COUNT          568 a          844 c          892 g          647 t
ORIGIN      
        1 tcgaggatat gcttcgggga gagtccgtcg attaagtctt tttgttggcg ccggaccgtc
       61 ctttgtggcg ggcccggtat gctgtgtttt tcaggagtga aaacgaagga gcgggggatg
      121 ttttcgtgac gtggagcagt gcgtttcaaa ttctcggggg cgttggactt tttcttttcg
      181 gtataaaact gatgagcgag gcgctccagt acctcgccgg cgacaagatg cgccagctta
      241 tcggttccct gaccagaact cccatcagag gcgtttttat cggcgcgctc gtcgccatgc
      301 ttatccagag cagcagcggc acgaccgtta tgacggtgag cttcgtccac gcggggttga
      361 tgaccttgaa acaggccgtc ggcgttatca tgggtgcgaa catcggcacc acggtcatcg
      421 cgcagatagt tgctttcaag gtaaaggact tcgcgctccc catacttggt atcggggttc
      481 tgctggtgct tttcggaagg acgaaacggc agaagtacat cggtaacggt ctcgtcggct
      541 tcggtctgct cttccttggt atgcagacga tggagggctc catgtccttc ctgcgcgacc
      601 gaaaggacct ttttattgct ttcagctcct cgccgatgct cggtcttttc gtcggtacgg
      661 cggtcacaat ggtagtgcag gcgagctccg cgacgatagg cctcacgatg gccatggctg
      721 cgcaggggct tctgccgctt gaagccgccc tccccattct cctcggcgac aacataggca
      781 ccacgatcac cgcggtgctc gcctcgctcg gctccaacag agccgccaag caggccgcga
      841 cggcgcacgt gctcttcaac gtcatcggcg ccgcgctctt cctcgcgttt ttgccgttct
      901 acaaggacgt ggtggccgcc acctctacgg acatcggacg gcagatagcc aacgcgcaca
      961 ccatcttcaa cgtgacgaat acgctgctct tccttccttt cgtccccgtc ctggtgtggg
     1021 tcatacgcag gatcgtcccc gacaagggag gcgctgtcgt cgcggggcct atgtacctgg
     1081 acttgaagct catatcggcg tctcccgcag ccgcagtgga cgcggtaaaa aaggagctcc
     1141 tccacatggg ggatcttgcc cgttcgatgc tgacagatgt tcgcagggcc ttcacggaga
     1201 acaatccgaa gatgatcggc gaggtgacgc agacggagaa agccgtgaac gagatcaacc
     1261 gcgccatctc ctcctacgcg tcggaagtct ggcagggagg gctttcgagc gaactttcga
     1321 cggtactcgg ctcgtacgtc aacggcgtcg gcgacataga acgcatcggg gatcatgcga
     1381 ccaacctgat cgagctctat gaatacaagc ttgatcacgg cgttgagttc tccacgctcg
     1441 cccgagggga gttcgacgac atgttcgacc tcgttgaacg ggcggttcgt ctgagtttcg
     1501 agtcgctcaa cgaggaggac gtcattaagg ccaaggaggt cgacgctctc gaggacgagg
     1561 tggaccgcaa ggagaagatt ctccggaaaa accatattct ccgcctcaac aagggggagt
     1621 gcactcccgc cgggggcgtc atcttcatcg acatcctgag caatctcgaa cgagtgtgcg
     1681 atcacgccca caatatctcg ctcatcgcgt tcgatatatc aaagctgcac agatgacgga
     1741 cgaccgggct tcttgaggtg gacgaatatt ggtatgtctt gaaaccggcg gggaggctcc
     1801 tcgccggttt tttgaacgag ttttttcatg ctccctcctt gacataccgg gtgcattcgc
     1861 tattctgtag acggttcttg ttttactcgg taaatttttg ttttaatgga taaaacaagg
     1921 aggagatgct atggaatgca cacacgacgg agcgcgctgt atcgtcctcc gggagaacga
     1981 cagcgtcgcg gttcttcccg agggaggagg cgccggatcg cggcttccct ttgccgagca
     2041 ggagctcgtt cttaaagagg ttatcgctcc cggacacaag attgctctcc gggacatcaa
     2101 gcagggggag cgggtggtga agtacggttt tccgatcggc gttgcgagcc gcccgatcgc
     2161 cgcaggcgag catgtgcatg tgcagaacgt ccgctccgct ctcggacacg aatggtcgcc
     2221 gtcgtggagc ccttctcctg cgcgccttct ctcccagcta tcgcccgttc cttcatttca
     2281 aggataccga agaacggagg ggcctccggg catacggaac gagctctgga tcattccgac
     2341 ggtcgggtgc gtcaacgagt acctgaaatt cctcgccgga gcgtggacgg ccccttcctg
     2401 gatcgaaaac gtccgcgtgc tttcccaccc ctacggctgc tcccagctcg gggaggattt
     2461 ggaacggacg gcagccgtcc ttgccgggct tgcgcgcaat ccgtgcgcgg ccggggtgat
     2521 tatcgcgggg cttggatgtg aaaacctcca gcgcgtcttt ctggagaccc gccttgcagg
     2581 agtggaaaaa gtccgattcc tctcgctcca ggaggaggac gacgatgccg gagccttgta
     2641 cgctctcctc gatgagcttg cggcggatgc tccgaaagag agagaaccat tccccctctc
     2701 cggggtgacg ctcggtgtga agtgcggcgg cagcgacggc ttctccggtc tttcagccaa
     2761 tcctctcgtc ggcgaagtcg ccgacagaat gagcgcgtgg ggggggaaga tcctcgccac
     2821 cgaaatacct gagatgttcg gcgccgagga tgtcatcgcc tctcgaatag ccgacaggca
     2881 ggtcttcgat gcctttgcgg agaccattcg ctggttccgg aactactatg tggaacacaa
     2941 gcagccggtg t
//
LOCUS       DAOTIL010000279         2949 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminobacteriaceae bacterium isolate AMR_MDS_3026
            NODE_39780_length_2949_cov_3.010366, whole genome shotgun sequence.
ACCESSION   DAOTIL010000279 DAOTIL010000000
VERSION     DAOTIL010000279.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868050
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminobacteriaceae bacterium (wastewater metagenome)
  ORGANISM  Aminobacteriaceae bacterium
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae.
REFERENCE   1  (bases 1 to 2949)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2949)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:19:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,224
            CDSs (total)                      :: 3,198
            Genes (coding)                    :: 3,184
            CDSs (with protein)               :: 3,184
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2949
                     /organism="Aminobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_39780_length_2949_cov_3.010366"
                     /isolate="AMR_MDS_3026"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:3034609"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(98..>306)
                     /locus_tag="P5201_06715"
     CDS             complement(98..>306)
                     /locus_tag="P5201_06715"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRV98263.1"
                     /translation="EAAILMLRTARGISFEAFTSRYGEETLSSLLRTLEENAPPDCFV
                     RSDASFALSPKGMRVAHALWRLIV"
     assembly_gap    307..316
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<317..1277)
                     /gene="hemW"
                     /locus_tag="P5201_06720"
     CDS             complement(<317..1277)
                     /gene="hemW"
                     /locus_tag="P5201_06720"
                     /inference="COORDINATES: protein motif:HMM:TIGR00539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051989 - coproporphyrinogen dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM family heme chaperone HemW"
                     /protein_id="HRV98264.1"
                     /translation="MRNNVGEEIVHRLAVSGQEFSLYIHLPFCARKCPYCSFFSVVPR
                     NGEIEEYCRLLHSEIRFFGERLAGSGALAGTIYFGGGTPSLLAPADWERLFKCLRKHI
                     LFDQKVEITVEANPDSLSESHVRLWKEQGVSRISVGVQSFIDGELRWLGRLHDADRAR
                     MALAMCVHSGISVSADFMFGLAGQTLRSFSTSIAEAIALGAEHLSLYQLSIDETSRWS
                     DNPPEGRKDGYPFYRWSQWYLPKKGFAQYEISSFARPGKECRHNSAYWTNGAVLALGA
                     GAWGYADGIRYRNERTLIGYAEAIRRNGHSVAEAETPDACKRAH"
     gene            complement(1267..>2949)
                     /gene="lepA"
                     /locus_tag="P5201_06725"
     CDS             complement(1267..>2949)
                     /gene="lepA"
                     /locus_tag="P5201_06725"
                     /EC_number="3.6.5.n1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013048547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation elongation factor 4"
                     /protein_id="HRV98265.1"
                     /translation="LDTLDLERERGITIKLVPVRMKYRAEDGEDYILNLIDTPGHVDF
                     GYEVSRSLAACEGALLVVDATQGVEAQTLANAYMAVDQGLEILPVINKIDLPSSRPDY
                     VIKEIEDVVGLDCDKVILASAKEGKGVPEILERIVHGVPAPSGNPEAPLQAMIFDSKY
                     DNYKGVICYIRVMNGTLRPGRNIRFMATGGVFPLEEVGVLRPNFEPCDELTAGEVGYI
                     VSNIKTVAEAHVGDTITEDARPATAALPGYRKVKPVVFCGFYPVERDDYPQLRDALEK
                     LTLNDSSISFEPETSLALGFGFRCGFLGLLHMDVSKERLRREYSVELVATSPNVIYEL
                     TTKSGEIIEAHRPSDFPEIGDIEEVREPMIKLTLFVPSDYVGKVMKLCQDKRGTYISM
                     DYLAPDRVRLIYEVPLAEFIIDFHDKLKSHTRGYASLDYEHTGLKASDLVRVDVLING
                     EAADAFSFICHREAAYNRGQAVVRKLKELIPSQLFEVPLQASIGKKVIVRQNVKPLRK
                     DVLAKCYGGDITRKRKLLEKQKEGKKRMKQIGRVSIPQEAFLAFLKVEEDEE"
BASE COUNT          680 a          844 c          751 g          664 t
ORIGIN      
        1 agcacaatca aaatatcctc aactacgtag gaatcctcgg gagtgaaacg tatcatgccg
       61 tattccatcg gcagcatctt cctttcctga acgctgatta cacaataagc ctccacaggg
      121 cgtgtgcgac cctcatcccc ttcggggaga gcgcaaaaga tgcgtcgctc cgaacaaaac
      181 aatcgggagg cgcattctcc tccagagtac gaagaagtga cgagagtgtt tcttctccgt
      241 accgcgaggt aaaagcttcg aagctgatac ctctggcagt ccgaagcatc aggattgcag
      301 cttcatnnnn nnnnnncatg cgcacgctta caagcatccg gagtttcagc ttcggcaacc
      361 gagtgtccgt tgcgccggat tgcttcagca tatccgataa gcgtccgttc gttgcgatag
      421 cgaataccat ccgcgtaccc ccacgctccc gcgccaagag cgagcaccgc gccgttcgtc
      481 cagtaggcgc tgttgtgccg gcattccttc cctggccggg caaagcttga gatttcatac
      541 tgggcaaaac cttttttcgg aagataccac tggctccacc tgtagaacgg gtatccgtct
      601 ttccgccctt caggcggatt gtcggaccat ctgctggttt catcgatcga gagctgatac
      661 agcgagagat gttcagctcc gagagcgatt gcctcagcga tcgaggtgga aaaggagcgt
      721 aaagtctgac cggcaaggcc gaacataaaa tctgcgctca ccgaaatgcc tgagtgaacg
      781 cacattgcca acgccatcct ggcgcggtcc gcgtcatgga gtcgtccaag ccagcgaagt
      841 tcgccgtcaa taaagctctg cacaccgacg ctgattctgg agacaccctg ctctttccag
      901 agacgcacgt gactttcaga aagggagtcg ggatttgcct ccacggtgat ctctaccttt
      961 tgatcgaaaa ggatatgctt gcgtaaacat ttgaaaagcc gctcccaatc agccggagca
     1021 agcagcgacg gggtgcctcc tccgaagtat atagtccccg cgagtgctcc gcttcccgca
     1081 agacgctctc cgaaaaaacg gatctccgaa tgcagaagcc tgcagtactc ttctatttcc
     1141 ccgtttcgcg gaaccacgct gaaaaacgaa cagtagggac atttccttgc acagaacggc
     1201 agatgaatat aaagagaaaa ctcctgtccg gagacggcga gccggtgaac aatctcctcg
     1261 ccgacgttat tcctcatcct cttctacctt cagaaatgcg agaaaagctt cctgggggat
     1321 agacacgcgc ccaatctgct tcatgcgctt ctttccctct ttctgctttt caaggagttt
     1381 ccgttttcgg gtaatgtcgc ctccgtagca cttcgcaagc acgtcttttc gaagcggctt
     1441 tacattctgt cgaacaatga cttttttccc tatggacgcc tgaagaggca cttcaaaaag
     1501 ttggctcgga atcagttcct tgagttttcg caccacagcc tggccccgat tgtaggcggc
     1561 ttccctgtgg cagatgaagg agaacgcatc ggcggcttct ccgttgatca gcacatcgac
     1621 acgaaccagg tcggacgcct tcagaccggt atgctcgtag tcaagggacg cataccccct
     1681 tgtatgggac ttcagcttgt cgtggaaatc aatgataaac tccgccaacg gcacttcata
     1741 gatcaagcgt acacggtccg gggcaagata gtccatggag atatacgttc cccgtttatc
     1801 ctgacagagc ttcatcacct ttccaacgta gtccgacgga acgaagagag tcagtttgat
     1861 catcggctcc cgcacttctt cgatatcacc gatctcagga aaatcggagg gacggtgggc
     1921 ttcaatgatc tcgccgctct tcgtcgtcaa ttcatatatg acgttcggtg aagtggcgac
     1981 gagctccacg ctgtactcgc ggcgaagacg ttcctttgac acatccatgt ggagaaggcc
     2041 gagaaacccg caacggaacc cgaatccgag agcaagcgaa gtctccggtt caaaggagat
     2101 cgaggagtcg ttaagcgtca gcttttcaag agcgtcacga agctgagggt agtcgtcgcg
     2161 ttccacggga tagaagccgc aaaaaacgac aggtttgacc tttctgtagc cggggagcgc
     2221 cgccgtcgcc ggacgcgcat cctccgtgat ggtgtctccc acgtgcgctt ctgccacggt
     2281 cttgatgttc gatacgatat agccaacctc tcccgccgtc agttcgtcgc acggttcaaa
     2341 gttcgggcgc agcacgccga cttcctccag gggaaaaacg ccgccggtcg ccataaaccg
     2401 gatattgcgg ccggggcgca gcgttccatt cataacgcgt atgtaacaga taacgccttt
     2461 gtaattatcg tactttgagt cgaagatcat cgcctgcaac ggcgcttcag gattgcccga
     2521 cggcgcgggt actccgtgaa cgatgcgctc cagtatctcg ggaactcctt tgccctcctt
     2581 ggcgcttgcg agtatcacct tgtcgcagtc aagcccgaca acatcttcta tttccttaat
     2641 tacatagtcg gggcgggatg acggaaggtc gatcttgttg atcaccggga gaatctccaa
     2701 cccttgatca accgccatat acgcgttggc gagagtctgc gcctcaacgc cctgcgtggc
     2761 gtcgacaacg agcagcgctc cttcacaggc tgcaagagag cgggagacct catatccgaa
     2821 gtccacatgc cccggagtat cgatcaggtt gagaatatag tcttctccgt cttcggctcg
     2881 atatttcatg cgtaccggaa caagcttgat ggtgatcccc cgttctcttt caagatcgag
     2941 ggtatcaag
//
LOCUS       DAOTIL010000280         2947 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminobacteriaceae bacterium isolate AMR_MDS_3026
            NODE_39813_length_2947_cov_3.643499, whole genome shotgun sequence.
ACCESSION   DAOTIL010000280 DAOTIL010000000
VERSION     DAOTIL010000280.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868050
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminobacteriaceae bacterium (wastewater metagenome)
  ORGANISM  Aminobacteriaceae bacterium
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae.
REFERENCE   1  (bases 1 to 2947)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2947)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:19:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,224
            CDSs (total)                      :: 3,198
            Genes (coding)                    :: 3,184
            CDSs (with protein)               :: 3,184
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2947
                     /organism="Aminobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_39813_length_2947_cov_3.643499"
                     /isolate="AMR_MDS_3026"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:3034609"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..280
                     /locus_tag="P5201_06730"
     CDS             <1..280
                     /locus_tag="P5201_06730"
                     /inference="COORDINATES: protein motif:HMM:NF021390.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="MliC family protein"
                     /protein_id="HRV98266.1"
                     /translation="VMGGEPVVYDCNNGDEVVARYYMLSDESLRFVKLLLPDGTEYTL
                     PQVLSGSGARYTDDIELLWWIKGDDAYVEKRDETGEWRPLYSECRARP"
     gene            complement(445..687)
                     /locus_tag="P5201_06735"
     CDS             complement(445..687)
                     /locus_tag="P5201_06735"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRV98267.1"
                     /translation="MASFPQATARVAVGELIGKIRAHYGKSVETNIYDPRCLFWFFDL
                     VRFNIRAEPTWVFDGKLLFRGIPPWEELREKMDATL"
     gene            complement(781..1560)
                     /locus_tag="P5201_06740"
     CDS             complement(781..1560)
                     /locus_tag="P5201_06740"
                     /inference="COORDINATES: protein motif:HMM:NF038196.1"
                     /note="N-terminal region resembles flavodoxins. C-terminal
                     ferrodoxin region binds two 4Fe-4S clusters; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EFR1 family ferrodoxin"
                     /protein_id="HRV98268.1"
                     /translation="MKESSVDLYFFSGTGNTLLAARAVAEGLRAGGKSVRLLRLEKGI
                     SAPAGDTALGVAVTTACFSTYPFVWEALEQLPSGEGRSAFAISTMGGYSGGLRSPLRK
                     LLHKKGYRTLGYAEFVMPSNYANKSIPQEANREKIQKCEAKAREFALALLEDRATWRS
                     GGPFSPFFNWMARKKAPWRMMKRSFPLEIDKSRCIQCGKCARLCPAKNIVMEEYPRFL
                     DHCVVCQRCIAFCPPAAIGVHGKKYEQYRAVEYDTLVSDKL"
     gene            1853..2410
                     /locus_tag="P5201_06745"
                     /pseudo
     CDS             1853..2410
                     /locus_tag="P5201_06745"
                     /inference="COORDINATES: protein motif:HMM:NF013766.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0008113 - peptide-methionine (S)-S-oxide
                     reductase activity [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase"
     gene            complement(2421..>2947)
                     /locus_tag="P5201_06750"
     CDS             complement(2421..>2947)
                     /locus_tag="P5201_06750"
                     /inference="COORDINATES: protein motif:HMM:NF013629.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="transcriptional repressor"
                     /protein_id="HRV98269.1"
                     /translation="PHALRSPPMGRRRSLQKVPYMQLSRVRLCREVMEPAALLRAREI
                     RPTPLRKEALALLFAAGVPLSLKTLSERFQGKPPDRVSLYRTLTLLELKGIVHKVLGA
                     DGAWRYCAHPPEESGCPGNHAHFLCLECGSMFCLQDQPIPVLTLPDGCVVEGKQLLAY
                     GLCKECAELRTKGK"
BASE COUNT          632 a          798 c          837 g          680 t
ORIGIN      
        1 cgtcatgggg ggagaacctg tggtgtacga ttgcaataac ggggatgagg ttgtcgctcg
       61 gtattacatg ctctctgacg aaagtctccg tttcgtcaag ctgctcttac cggacggaac
      121 cgagtatacg ctgccccagg tgctttccgg ctcgggagcc cggtacactg acgatatcga
      181 actgctctgg tggataaaag gagacgacgc atacgtcgaa aagcgggacg agacgggcga
      241 atggcgtccc ctgtacagcg agtgccgcgc acggccgtaa cggttttgaa ctgacggaag
      301 aagctccctg cacggtttgc cctggatcaa tgcgccgagt tccgtgcacg ggagcttcct
      361 ttctggagac gaagcgtatg aacctgatgt tgtcatgacc gcgttcggta gtggagtacc
      421 caatttgctg ctttgcagcg cggttcaaag cgtcgcatcc atcttttccc gcagttcttc
      481 ccagggaggg atccccctga agagcagttt tccgtcgaaa acccacgtcg gttccgcacg
      541 gatattaaac cgcaccaggt cgaaaaacca gaaaaggcat cgggggtcgt agatatttgt
      601 ctcgacgctc ttgccgtaat gcgctcgaat cttgccgatc agctctccca ccgccacacg
      661 ggcggttgcc tgcgggaagg aggccatctc ctcgcttccg tacagaaatc cgcttcagca
      721 gacggaggaa atcggttttt ccatgtagat tttgaggcga tgccgcatag gcttcactcc
      781 ctatagtttg tccgatacga gcgtatcgta ttccaccgcc ctgtactgtt cgtacttctt
      841 cccatgaacg ccgatggcgg caggagggca gaaagcgatg catcgctggc agacgacaca
      901 gtgatcaagg aaacgcgggt actcctccat gacgatgttt ttcgcagggc aaagacgggc
      961 gcactttccg cactgaatac acctgctctt gtcgatttcg agcgggaacg agcgtttcat
     1021 cattctccat ggcgctttct ttcgcgccat ccagttgaaa aacggcgaaa acggtccccc
     1081 gctgcgccat gtggcgcgat cttcgagaag cgcgagcgca aattcgcgag ccttcgcctc
     1141 gcatttctgg attttttccc tgttcgcttc ctgcgggata ctcttattcg cgtagtttga
     1201 cggcataacg aattccgcat acccgagcgt cctgtacccc tttttgtgga gcagtttacg
     1261 aagaggcgat cgcagccccc ctgaataccc tcccattgtg gagatcgcga atgcgctccg
     1321 tccttctcct gaaggaagct gctccagagc ctcccagaca aacggatagg tcgagaaaca
     1381 tgcggtcgtt acagcgacgc cgagcgccgt atcccccgcc ggcgccgata tccccttttc
     1441 gaggcggagc agacggactg atttcccccc tgcgcgaagc ccttcggcta ccgctcgtgc
     1501 agcgagaagt gtgtttcctg taccagagaa aaaatacaga tccacggatg attctttcat
     1561 tgtgtctcct ttcttctttc cttacggaga gtgatgatga agtaattcta tcacttccgc
     1621 cataatgctg atcttttaca gggtacaatc tgagctaaag tagtgtatca tagtggatga
     1681 aacaaagaat ttggaggtga gagagatgag aatattggag tcgttgcttc tttttgcgct
     1741 gttcttctcc gcggagaagg cgtacgcagc ttttcggcct cctctggatg aaagcgctcc
     1801 ggaggttttt cagacagcgt cctttgctct tggctgattt tggggaccgg atgcccggtt
     1861 cgggcttctt gaaggcgtga tccgtacccg cgtcggctat gcgggaggaa cgacgccgga
     1921 ccctacctac gaagagatag gagatcattc ggagacaatt cagatcgatt acgatcccga
     1981 agcgctctcg tacgaagagt tgctgagtgt cttctggagc agtcataacc cgtcggctcc
     2041 ttcctggtcg acgcagtacc gttccgccat ctttgtagca aacgaagagc agcgtcgtgc
     2101 agcggaggaa agccgtgaaa aggtgaaaac gtctcgcggg ggtggagtgc agacttccat
     2161 agagccgctg aagcgcttct atccggcgga agactatcac cagaagtact acctgcggca
     2221 ggagcacgtg ttgatgacgg atttcatgaa taactacgcc actgcggagg attttatgaa
     2281 ctctcccgcg gcgacgaagg tgaacggctt cctttccggg actggttcag aggagtattt
     2341 aaaaaaaata ctccccggtc ttggtctttc ggaagaaggg gagaagagac tcaggcttcg
     2401 cgtccgttga acaggagcgt ttattttcct tttgtgcgaa gttctgcgca ctccttgcag
     2461 agaccgtagg cgagcagctg tttgccctct accacgcagc cgtccggtag cgtaagcaca
     2521 ggaatgggct ggtcttgaag gcagaacatg gagccgcatt ccaggcagag aaagtgcgcg
     2581 tgatttccgg ggcagccgct ctcttccgga gggtgcgcac agtatcgcca agccccgtcc
     2641 gctccaagga ctttgtggac gattcctttc agctcaagga gcgtcaatgt cctgtacaac
     2701 gaaaccctgt cgggcggctt cccctggaag cgctcggaca gggttttgag agaaagaggg
     2761 actcccgctg cgaaaagaag cgcgagcgct tcttttcgca gcggcgttgg acgaatttct
     2821 cgtgccctca ggagcgcggc gggttccatt acctcgcggc aaaggcgcac acgggatagt
     2881 tgcatgtacg gcactttctg caaagaccgc ctgcgcccca tcggcggact tcggagagcg
     2941 tggggat
//
LOCUS       DAOTIL010000281         2943 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminobacteriaceae bacterium isolate AMR_MDS_3026
            NODE_39889_length_2943_cov_3.669321, whole genome shotgun sequence.
ACCESSION   DAOTIL010000281 DAOTIL010000000
VERSION     DAOTIL010000281.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868050
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminobacteriaceae bacterium (wastewater metagenome)
  ORGANISM  Aminobacteriaceae bacterium
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae.
REFERENCE   1  (bases 1 to 2943)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2943)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:19:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,224
            CDSs (total)                      :: 3,198
            Genes (coding)                    :: 3,184
            CDSs (with protein)               :: 3,184
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2943
                     /organism="Aminobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_39889_length_2943_cov_3.669321"
                     /isolate="AMR_MDS_3026"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:3034609"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..753
                     /locus_tag="P5201_06755"
     CDS             <1..753
                     /locus_tag="P5201_06755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006300744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRV98270.1"
                     /translation="ALVFYVVFVTKPPVMETVHEMIRPLTSLDILKEYKWLVVLTLVG
                     GTVGGYISFSGAHRIIDAGIVGKENIGQISKGSINGIAITGVMRYLLFLAFLGVVLTG
                     TPIDMTNPVASAFQIGAGALGFRIAGVVLWAAAITSVVGASYTSVSFLRTLFKVVDNY
                     YRYWIIAFIVASTSILTTVGRPVALLIVAGSLNGLILPLSLGCVLMAAHNKNIVKDYM
                     HPVWMTALGWVIVVFTAWMGFNSFAGIMKLFQ"
     gene            791..1561
                     /gene="pxpB"
                     /locus_tag="P5201_06760"
     CDS             791..1561
                     /gene="pxpB"
                     /locus_tag="P5201_06760"
                     /EC_number="3.5.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015555942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-oxoprolinase subunit PxpB"
                     /protein_id="HRV98271.1"
                     /translation="MNEKKYPRLLTAGDGCVVVEFGDVIDMEINSRVASLAAAVRKAE
                     FPGFLDTVPTYRSLAVYFNPVTTDVDALYSKLGKMASALGTGKGSESRRVIVVPSLYG
                     GEHGPDLADVAEHTGLTPEEVVKRHTANDCYCYMLGFTPGFAYLGGMDPSLETPRLKN
                     PRELIPAGSVGIAGKQTGIYSISSPGGWRLIGRTPMRMFDPDRNPAIFLEAGMWVRFR
                     AVGKAEFDEIEAATAARTYRPEILEEVSPRLVGAGEVL"
     gene            1564..2658
                     /locus_tag="P5201_06765"
     CDS             1564..2658
                     /locus_tag="P5201_06765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005660493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin-dependent carboxyltransferase family
                     protein"
                     /protein_id="HRV98272.1"
                     /translation="MAALAFSVQMPGMLTTVQDSGRWSYQGKGMPVAGAMDMQSFALG
                     NILVGNDENAAALEVTLLGPTLSVTEGEGVAAVTGAELGFTVNGEPIANWTVVTIREG
                     DILSFDGPRSGCRAYLCVSGGIDVPMVMESRSTYTRAKVGGLEGRALKKGDAVRCGEP
                     APLWNRCEGLVCPEALRPLRDPAAPLRVIPGPQDDLFTEKGLASFYGTEYVISNSADR
                     MGYRMDGEPIEHTGAADIISDAIPLGAVQVPGHGQPIVMLADRQTTGGYTKIGVVCSV
                     DVAALSQRLPGAKVRFSKIAVKDAVGLVRAEARTRDELRKLRAAWRSSARVSSAPVPE
                     AVAAPTGGEAILRVDGKEHVISWEEIGEVE"
     gene            2658..>2943
                     /locus_tag="P5201_06770"
     CDS             2658..>2943
                     /locus_tag="P5201_06770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014807623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LamB/YcsF family protein"
                     /protein_id="HRV98273.1"
                     /translation="MFKIDLNSDLGESFGPWRMGMDREVMEHVSSANVACGFHAGDPE
                     VMIRTVQAAKEASVAVGAHPGHPDLQGFGRRTMGCTPDEVYAFTLYQIGAI"
BASE COUNT          537 a          900 c          948 g          558 t
ORIGIN      
        1 gctctcgtct tctacgtcgt cttcgtcacg aagcctcctg ttatggagac ggtgcatgag
       61 atgattcgcc ccttgaccag cctggatatc ctgaaggagt acaagtggct cgttgttctc
      121 accctcgtcg gcggcacggt cggcggctac atctccttct ccggcgcgca ccggatcatc
      181 gacgccggaa tcgtcggcaa ggagaatatc ggccagatca gcaaaggctc catcaacggc
      241 atcgccatca ccggcgttat gcgctatctg cttttcctcg ccttcctcgg cgtggtgctc
      301 accggaaccc ccatcgacat gaccaacccc gtcgcctcgg ccttccagat cggcgcaggc
      361 gccctgggct tccgcatcgc cggagtcgtc ctctgggccg cggccatcac ttcggtcgtc
      421 ggcgcgtcct acacgtcggt ctccttcctg aggacgctct tcaaggttgt cgacaactac
      481 taccgttact ggatcatcgc gttcatcgtc gcctccacat ccatcctgac caccgtcggc
      541 cgtccggtcg ccctgcttat cgtcgccggt tcgctcaacg ggctgatcct tcctctgtcg
      601 ctgggctgcg ttctgatggc ggcgcacaac aagaacatcg tgaaggacta catgcacccc
      661 gtatggatga cggcgctcgg ctgggtcatc gtcgtcttca ccgcgtggat gggcttcaac
      721 tccttcgccg ggatcatgaa gctgttccag taggaagaag aaagatattt atgaaggagg
      781 cggtgtccgc gtgaatgaga agaagtatcc gagactgctg acggcgggcg acggctgcgt
      841 ggtcgtcgaa ttcggcgacg tcatcgatat ggaaataaac tcgcgcgtcg cctcgctcgc
      901 cgcggcggta cggaaggcgg aattccccgg gtttctggat accgtcccga cgtaccgctc
      961 gctggcagtc tatttcaatc cggtcacgac cgatgtcgac gctctctact cgaaactcgg
     1021 gaagatggcc tcggcgctgg gaacaggtaa aggaagcgaa tcgcgccggg tcatcgtcgt
     1081 gccctccctc tacggcggag agcatggtcc cgatctggcg gacgtcgcgg agcacaccgg
     1141 cctgacgccg gaagaggtcg tcaagcggca cacggcgaac gactgctact gctatatgct
     1201 cggctttact ccgggcttcg cctacctcgg cggaatggac ccgtcgcttg aaacgccgcg
     1261 gctgaagaat cctcgggagc tcatccctgc gggttccgtg ggtatcgcag gcaaacagac
     1321 gggaatctac tcgatttcaa gcccgggagg gtggcggctg atcggccgca cgccgatgcg
     1381 gatgttcgac cccgaccgga atcccgccat cttcctggag gcggggatgt gggtgcgctt
     1441 ccgtgcggtc ggcaaggccg agttcgacga aatagaagcg gccacggcgg caagaacata
     1501 ccggccggaa atactggaag aggtctcgcc ccggctggtc ggggccgggg aggtgttgtg
     1561 attatggcag ctcttgcttt ttcggtacag atgcccggga tgcttaccac ggttcaggac
     1621 tccggacgat ggagctacca ggggaaggga atgccggtgg cgggggccat ggatatgcag
     1681 tcgttcgcgc tcggcaacat cctcgtaggc aacgacgaaa acgccgccgc tctcgaagtc
     1741 accctgctgg gacccaccct ctccgtaacg gagggggaag gggttgccgc cgtcacgggc
     1801 gcggagctcg gtttcaccgt caacggcgag cccatcgcga actggactgt tgtgacaata
     1861 cgcgagggag acattctctc cttcgacggt ccgcgctccg ggtgcagggc gtacctctgc
     1921 gtctccggag ggattgacgt cccaatggtc atggagagcc gctccacata tactcgcgcg
     1981 aaggtgggcg gtctggaagg gcgcgccctt aaaaaaggcg atgcggtgcg atgcggcgaa
     2041 ccggcgcccc tctggaatcg atgcgaaggg ttggtttgtc ccgaggcgct gcgtcctctc
     2101 cgcgatcccg cagccccgct gcgcgtcatt cccggtcctc aggatgacct gttcaccgag
     2161 aaggggcttg cctctttcta cggcacggag tatgtcatct ccaacagcgc cgaccggatg
     2221 gggtatagga tggacggcga accgatagag cataccgggg ctgccgacat catctccgac
     2281 gccattccct tgggagccgt gcaggtcccc ggccacggac agcctatcgt catgctggcc
     2341 gaccggcaga cgacaggagg gtacacgaag ataggcgtcg tgtgctcggt cgacgttgcc
     2401 gcgctttcgc agcgtcttcc cggagcgaag gtgcgctttt cgaaaattgc agtaaaggac
     2461 gccgtcggcc ttgtgcgcgc agaggcgcgg acgagggacg agctgaggaa actccgggcg
     2521 gcgtggcgct caagcgcgcg cgtatcctct gcgcccgtac cggaagctgt cgcggcgccg
     2581 accggcggcg aggcgattct ccgcgtcgac ggaaaggaac acgtgatctc ctgggaggag
     2641 atcggggagg tggaatgatg ttcaaaattg acctgaacag cgacctcggc gagagcttcg
     2701 ggccgtggcg catgggaatg gatcgcgaag tgatggagca cgtcagctcc gcaaacgtgg
     2761 cgtgcggttt ccacgcgggc gatcccgaag taatgatccg cacggtgcag gcggccaaag
     2821 aggcctccgt cgccgtggga gcgcaccccg gacaccccga ccttcagggg ttcggacggc
     2881 ggacgatggg gtgtaccccc gatgaagtgt acgcattcac gctttaccag atcggcgcga
     2941 tcc
//
LOCUS       DAOTIL010000282         2943 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminobacteriaceae bacterium isolate AMR_MDS_3026
            NODE_39890_length_2943_cov_3.561288, whole genome shotgun sequence.
ACCESSION   DAOTIL010000282 DAOTIL010000000
VERSION     DAOTIL010000282.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868050
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminobacteriaceae bacterium (wastewater metagenome)
  ORGANISM  Aminobacteriaceae bacterium
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae.
REFERENCE   1  (bases 1 to 2943)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2943)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:19:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,224
            CDSs (total)                      :: 3,198
            Genes (coding)                    :: 3,184
            CDSs (with protein)               :: 3,184
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2943
                     /organism="Aminobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_39890_length_2943_cov_3.561288"
                     /isolate="AMR_MDS_3026"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:3034609"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..475)
                     /locus_tag="P5201_06775"
     CDS             complement(<1..475)
                     /locus_tag="P5201_06775"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRV98274.1"
                     /translation="MYRLFSFAMVLFWILVQQTCLLCARWACDDLSGRKKPLALFLAA
                     QALLFLIVSTAAVSDLADWIPRLHEHTNNSPSMVWQFLCTVMLALDGGLIAYAWRFYR
                     LHALKGNLPADSALKLFVAWGTVCLLLYGAYFAPALSVATRHSLTLWEWEYICLFY"
     gene            837..>2943
                     /locus_tag="P5201_06780"
     CDS             837..>2943
                     /locus_tag="P5201_06780"
                     /inference="COORDINATES: protein motif:HMM:NF012311.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family serine peptidase"
                     /protein_id="HRV98275.1"
                     /translation="MNCNGRISLGKGEDEKLRGKSVSKSWGAFAEFIALDYRTMNSAR
                     ECLSASGFSLGEVFIRSISQHEWMVMGMKKTKLALILLFLFLCAGSAFGQPKAPSDEY
                     VRGDVLVVLEAPGGASEVESQANRIAEATSSRVIRTYSAIAAATGKNIVQLKADGKSA
                     EELLTAVRQIPGVLGATPNYIFKILRTPNDPEFPSSWGMESINASGAWEISTGSEEVY
                     VALLDTGIQYDHEDLAANMGRDKDGNFGRNLVSAANATNPMDDNGHGTHLAGIIGAAG
                     DNRLGAAGINWTVKLLAVKVLDGNGAASVGHIIAGLDYVLEQKSKGLNIRVANMAFGG
                     WTPPASDVAADPLAIACKSLSDAGILLVAAAGNEGQNIARAGGGYKGNTIYPAAFAFP
                     EMVTVAAITQNGALAKFSNFGSSVAIAAPGYEVKSTALGGTYGSLNGTSVASAHVAGA
                     AALLAAASPELTAGQIKTRLLEYAAGHQTLSGKVASGGSLNIGAAIKNERGSLKVLIE
                     GPASARWTLNGNGSYPSGQVLTGLPAGNHTVSFSDADLWSKPANMSVTVASGTEAEAI
                     GKYRQQNASIWVTIEGPEEARWSLNKKGEYTSDQTLGKAAGKRTVSFSEVPGWDAPAA
                     QSVTVPRNAPLAVSGVYTRQTGSVTVTFEGPAEARWSLDGQGSYTSGETVENVPVGNR
                     TVSFAKIEDWDTPQDVVVLV"
BASE COUNT          726 a          729 c          867 g          621 t
ORIGIN      
        1 aatagaagag acagatatac tcccattccc aaagagtcag cgaatgacgt gtagcgactg
       61 agagtgccgg ggcaaagtag gctccgtaga ggagcaggca cactgttccc caggcaacga
      121 agagtttgag agcactatct gcgggaagat tccctttcag agcgtgcaac cggtaaaaac
      181 gccaggcgta cgcaatgagt cctccgtcga gcgcgagcat gacggtgcag aggaattgcc
      241 agaccatgga gggagagtta tttgtatgct cgtggagccg gggaatccag tccgcgagat
      301 cgctgaccgc agccgtcgat acaatgagga aaagaagcgc ctgagctgca agaaaaagtg
      361 caagcggttt ttttctgccg ctcagatcat cgcacgccca gcgggcgcag agaagacagg
      421 tttgttgtac gagtatccag aagagaacca tagcgaagct gaacaggcga tacacatcac
      481 cacctccagc acaagaaaca gagacacttc aagggttgct gtaaaaagaa tcagcgtttt
      541 tttagaaaaa tgcttttgtt atatttgcat ttatgtatat actttgcctg ctcctctgtc
      601 aatagtatgg gaataattct ttcggaaagc gttctgccaa tgtgaagagt gattattaaa
      661 caatatagat attttgtgct tcagcccttt cggacgtgtt ttttgtcttt tctcattgaa
      721 atatcgaaat caagtactct acaacagaaa aaagttggta caattagagg tcaagaggaa
      781 gagagcttct tgaaaagctg tgttctgaaa accgtgaact acggatgtgc atttttttga
      841 attgtaacgg tcgaatttcc ttggggaagg gggaggatga aaagctgcga ggaaagagtg
      901 tttcgaagtc ttggggggct tttgccgagt tcatcgcact tgattacagg acgatgaaca
      961 gtgcccggga gtgtctgtcg gcgtccggtt tttcactggg cgaggttttt attcgatcta
     1021 tatcacagca cgaatggatg gtgatgggaa tgaaaaaaac gaaacttgcg ttgatcctgc
     1081 ttttcctgtt cctgtgcgcc ggctccgcct ttggccagcc gaaagcgccg agcgacgaat
     1141 atgtccgggg agatgttctt gttgttcttg aagcgcccgg gggcgcttcc gaggtagaga
     1201 gccaagcgaa ccggatcgcc gaagcgacct cttcgcgggt gattcgcacc tatagcgcga
     1261 ttgccgccgc aacagggaaa aatatcgtgc agctcaaggc ggacggtaaa tcagcggaag
     1321 aactgctcac cgcagttcgc caaattcccg gagtgctggg cgcgacgccg aactacatct
     1381 tcaaaatcct ccgaacgccg aatgatccag agtttccgtc ctcctggggt atggagtcta
     1441 taaacgcatc cggagcatgg gaaatatcca ccggcagcga ggaggtctat gtagctcttc
     1501 ttgatacggg aatacagtac gaccatgagg atctggcagc caacatggga cgcgacaaag
     1561 acggcaactt cggaagaaac ctggtgagcg cggcgaacgc gacaaatccg atggacgata
     1621 acggccacgg aacgcacctt gcgggtatta tcggcgcagc gggagacaac aggctgggcg
     1681 cagccgggat caactggact gtcaaactgc tggcggtcaa ggtccttgac gggaacggcg
     1741 cggctagtgt cggtcacatc atcgccggac tcgactacgt gctggagcag aaaagcaaag
     1801 gactcaacat ccgggttgcg aatatggcct tcggaggatg gactcctccg gcatccgacg
     1861 tcgctgccga tcctctggca atagcctgca aatccctctc cgacgcaggt attctcctgg
     1921 ttgccgcggc agggaacgag gggcagaaca tagcaagagc gggcggcgga tacaaaggga
     1981 atacgatcta tcccgcagcc tttgcgttcc cggagatggt gacagtagct gcgattacac
     2041 aaaatggagc gttggcaaaa ttctccaatt tcggatcctc agtagctatt gccgctcccg
     2101 gctacgaagt gaagagcacg gctttgggag ggacgtacgg ttctctcaac ggaacgtcgg
     2161 ttgcttccgc gcatgtcgcg ggcgcggcgg cccttcttgc ggcggcgtcc ccggagctga
     2221 cggcggggca gatcaaaaca cgtcttcttg agtatgccgc gggccatcag acgctctccg
     2281 gtaaggtagc gtccggcggt tccctgaata tcggcgccgc gataaagaat gagagaggtt
     2341 ctttgaaagt cctcatcgaa gggcctgcat cggctcgctg gaccttgaac ggaaatggga
     2401 gctatccaag cggccaggtt ctgactggtc tgcccgcggg aaatcatacg gtttccttct
     2461 cggacgcgga tctctggagc aagcctgcga atatgtcagt aacggtagct tccggtaccg
     2521 aggccgaagc gatcggaaaa taccgccagc agaacgcgtc catttgggtc accatcgaag
     2581 ggcccgagga ggctcgctgg agcctgaaca agaagggcga gtacacaagc gaccagactc
     2641 tggggaaggc cgccggaaag cgaactgttt ccttctccga agttcccgga tgggacgccc
     2701 ctgcggccca gtccgtaact gttcccagga atgcgccgct cgccgtgtcc ggcgtctaca
     2761 cgcgccaaac cggttctgtc acggtaacat tcgagggacc ggccgaagct cgctggagtc
     2821 ttgacggcca gggatcctat acgagcggag aaactgtaga aaatgttcct gtgggcaacc
     2881 gtacggtgag ctttgccaag atagaggact gggatacgcc tcaagacgta gttgttcttg
     2941 ttt
//
LOCUS       DAOTIL010000283         2931 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminobacteriaceae bacterium isolate AMR_MDS_3026
            NODE_40119_length_2931_cov_3.016342, whole genome shotgun sequence.
ACCESSION   DAOTIL010000283 DAOTIL010000000
VERSION     DAOTIL010000283.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868050
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminobacteriaceae bacterium (wastewater metagenome)
  ORGANISM  Aminobacteriaceae bacterium
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae.
REFERENCE   1  (bases 1 to 2931)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2931)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:19:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,224
            CDSs (total)                      :: 3,198
            Genes (coding)                    :: 3,184
            CDSs (with protein)               :: 3,184
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2931
                     /organism="Aminobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_40119_length_2931_cov_3.016342"
                     /isolate="AMR_MDS_3026"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:3034609"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1776
                     /locus_tag="P5201_06785"
     CDS             <1..1776
                     /locus_tag="P5201_06785"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025072.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HRV98276.1"
                     /translation="LVLDSQPITPAIEGGRARALGLYGNAGYDVRYLGVLEEGGEKRN
                     LRLTPTLEELDIPLSQEHLAERDALWKQPGGILALDVSFPLLGHLTPEYAATAERMLS
                     EANAVIFSRPWCYPLVSRALAEKMILTVYDAHNVEGVMRCALMDDGASGTELVGFVGE
                     LESSLCSRADLILAPSHRDRLSLARLYDVPHEKIRVVPNGAFTERVTPASEERKITLK
                     EAEGLTDAEKEIPVAVFMGSASQANVEAAEFILREVAPAMPHVVFLIFGSASEAVACT
                     APQNARLLGVVDEERKRDLLSMADVALNPLFSDCGGNAKMFEYMAAGLPVLATPPAVR
                     GIDLGGTEVVAFSSRERFVDDLRVLLEEPTELARSGAAGRCVAEERHSWEPISRDLGM
                     LIAWHASHRRRRRPFFSVVVPGYERPKLLSRMARLLSVQTYTDFEVLFIDQSRTPWNE
                     SGAYRPLDLLYHRIDVKGAARARNIGAFLSRGKVLVFLDDDCEPSPNWLAAARPWFDD
                     EANAGLEGMIRSDLGWNDDRGRGAQNDTVNSRTTNFFVRAEIFNKTGGFDDLRFAEGV
                     EPGLRLDGEGEVPMCRNAWIFRAPR"
     gene            1904..>2931
                     /locus_tag="P5201_06790"
     CDS             1904..>2931
                     /locus_tag="P5201_06790"
                     /inference="COORDINATES: protein motif:HMM:NF025684.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4384 domain-containing protein"
                     /protein_id="HRV98277.1"
                     /translation="MKRRSMVFPGVFLFFCVLAAPVFADVLPRTTTATRDIMLVEEFE
                     EKEEQTAVASPVWTGMDGEEQAARLGVEEMHKTDFSVSLSSGKSAQGDVYEIGDKIEF
                     TFTSDKDCYVTLLDFTPSGKIYVLFPNKWVSNNFVKAGESVVVPAAGQQFAMKLGGPA
                     GVDIVKAIATNVETKILDPENQKLFGPFSVLEDPKVATRDIMLIEEEPEAAAAASPET
                     PLEWTAASLAVFTKGDSPEKGGFGVSIEGNWIAKIWADRDAFLTGESIFVKLQSNRPA
                     TLVSLVNTGASGRENILLPEGVEKSVLPGAIAILPGKQDKWKLIAATEPGVDVLTAVL
                     RDESGAEM"
BASE COUNT          598 a          813 c          889 g          631 t
ORIGIN      
        1 ctcgttctgg actcgcagcc cattactccc gcaatcgaag ggggacgcgc cagagcgctc
       61 ggtctctatg gaaacgccgg atatgacgtc cgctacctcg gagttcttga ggagggcggt
      121 gagaagcgaa atcttcgcct cacgccgaca ctggaagaat tggacatccc tctctctcag
      181 gaacatcttg ccgagagaga cgcgctgtgg aaacagccgg ggggcatcct cgcgctcgac
      241 gtctcctttc cacttcttgg gcatctcaca cccgagtacg cagcaactgc tgagcgcatg
      301 ctctcggaag caaacgcggt gattttttcc cgtccgtggt gctatcctct tgtatccaga
      361 gcgcttgcgg aaaaaatgat cctgaccgtc tacgacgcgc acaacgtgga gggcgtgatg
      421 cgctgcgccc tcatggacga cggagcctcg ggtaccgagc tggtcggctt cgtcggcgag
      481 cttgagtcga gcctctgttc gagggcggac ctgattctcg ctccatctca tcgagaccgg
      541 ctttctttgg cccggctgta cgatgtcccg catgaaaaga tacgagtcgt tcccaacggc
      601 gcgttcacgg agcgcgtgac gccggcgagc gaggagcgaa aaatcacgtt gaaagaggcg
      661 gaggggctga cagacgcgga gaaagagatt ccggtcgcgg tcttcatggg aagcgcctct
      721 caggcgaatg tcgaggctgc ggagttcata ctccgcgaag ttgctcccgc aatgccgcac
      781 gtcgtcttcc tgatcttcgg gagcgcttcc gaagctgttg catgcacggc tccgcagaac
      841 gcccgtcttc ttggcgtcgt ggacgaagaa cgaaaaagag acctcctgtc gatggcggat
      901 gttgcgctca atccgctctt ttccgactgc gggggtaacg caaaaatgtt tgagtatatg
      961 gcagccggtc ttcccgtgct tgcaacgcct cccgccgtcc gcgggataga tctcggcggt
     1021 acggaagtcg ttgcgttctc ctcccgcgaa cgcttcgtcg acgatctgcg cgtcctgctc
     1081 gaagaaccca ccgagcttgc gcggagcggc gcagccggac ggtgcgtcgc cgaagaacgg
     1141 cactcctggg agccgatctc ccgcgacctg ggaatgttga ttgcatggca cgcttcgcat
     1201 cgcaggcgga gacgcccgtt tttttccgta gtcgttcccg ggtatgaacg tccaaagttg
     1261 ctttcgcgca tggctcgcct gctctctgtt cagacctaca ccgatttcga ggtgctcttc
     1321 atcgaccaga gccgtacccc atggaacgaa tcaggagcgt atcgccctct tgatctgctc
     1381 tatcacagaa tagacgttaa gggggcggcg cgtgcccgca acatcggcgc ctttctctca
     1441 cgaggaaagg tgcttgtctt tctcgacgac gactgcgaac cttcgccgaa ctggctggcg
     1501 gccgcgcgcc catggttcga cgacgaagcc aacgcggggc ttgaaggcat gatccgttcg
     1561 gacctcgggt ggaacgacga ccggggacgg ggcgcccaaa atgataccgt gaactcgcgc
     1621 acgacgaatt tttttgtcag agccgagatt tttaacaaga cgggaggttt tgacgacctt
     1681 cgttttgccg agggtgtcga gccgggattg cggctcgacg gggaaggcga agttcccatg
     1741 tgccggaacg cgtggatttt tcgggcgcca cgctgacttt ttccccgtgg agggggattt
     1801 tgtccggcgt tttattctgc cgtgtggtat aattcgactg agtgtcattt ttctgttttt
     1861 tgattcccgc tgttccggag aacgattaca ggaggtgttg cagttgaaaa gaagaagtat
     1921 ggtattccct ggagtatttc tgtttttctg cgtactcgcg gcgccggttt ttgcggatgt
     1981 tctgccccgg acaactaccg ctacacgcga cattatgctc gtcgaggagt tcgaggaaaa
     2041 agaggagcaa acggctgttg catcaccggt atggaccgga atggacggag aagaacaagc
     2101 cgcccgcctt ggcgtggaag agatgcacaa aacagatttt tccgtttcgc tctcttcggg
     2161 gaaatcagct caaggcgatg tctatgagat cggcgacaag atcgaattca ccttcacctc
     2221 cgacaaggac tgctatgtga cgcttctgga ctttacgcct tcggggaaga tttatgtgct
     2281 gttccccaac aagtgggtat cgaacaactt cgtgaaggca ggagagagcg tcgtcgtccc
     2341 cgcggcgggg cagcaatttg caatgaagtt gggggggcct gccggcgtgg atatcgtcaa
     2401 ggccatagcg accaacgtgg agacgaaaat acttgatcca gagaatcaga aactgttcgg
     2461 tccgttctct gtgctggaag atcccaaggt ggcgaccaga gacatcatgc ttatcgagga
     2521 ggaaccggaa gccgcggcgg cagcctcgcc ggaaacgcct ctcgaatgga ctgccgcttc
     2581 gctcgctgtc tttaccaagg gcgattctcc ggaaaaaggc ggtttcggcg tgtcgatcga
     2641 gggaaactgg atcgcgaaga tctgggcgga ccgcgacgcc tttctgaccg gcgaatccat
     2701 atttgtgaaa cttcaaagca accggcccgc gacgctcgta tctctggtga acaccggcgc
     2761 cagcggcagg gagaatattc ttcttcccga aggcgttgaa aaaagcgtgc tcccgggggc
     2821 catcgcgatt cttcccggca aacaggacaa atggaagctg atcgccgcca ccgaaccggg
     2881 agtcgatgtg ttgaccgcag tgctccggga cgagtccgga gccgagatga a
//
LOCUS       DAOTIL010000284         2930 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminobacteriaceae bacterium isolate AMR_MDS_3026
            NODE_40141_length_2930_cov_3.259826, whole genome shotgun sequence.
ACCESSION   DAOTIL010000284 DAOTIL010000000
VERSION     DAOTIL010000284.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868050
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminobacteriaceae bacterium (wastewater metagenome)
  ORGANISM  Aminobacteriaceae bacterium
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae.
REFERENCE   1  (bases 1 to 2930)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2930)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:19:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,224
            CDSs (total)                      :: 3,198
            Genes (coding)                    :: 3,184
            CDSs (with protein)               :: 3,184
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2930
                     /organism="Aminobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_40141_length_2930_cov_3.259826"
                     /isolate="AMR_MDS_3026"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:3034609"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..589
                     /gene="era"
                     /locus_tag="P5201_06795"
     CDS             <1..589
                     /gene="era"
                     /locus_tag="P5201_06795"
                     /EC_number="3.6.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013048156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="GTPase Era"
                     /protein_id="HRV98278.1"
                     /translation="VRTPVLLVVNKCDVVLDRKGGGEAALAVVRSLYEARISLCGVVF
                     VSARMGRGLDTLIAAIRPHLPEGPPFYDEDILVDRPEKFIAAEIIREKVLLMTHREVP
                     HSVAVEIEEYKSPDEYPERDVLFIRATLYVEREGQKRIVIGDSGARLKEIGRRARMDI
                     ETLTGHKVYLDLWIKVRKDWRQSDADLRMLGIQER"
     gene            595..1392
                     /locus_tag="P5201_06800"
     CDS             595..1392
                     /locus_tag="P5201_06800"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecO"
                     /protein_id="HRV98279.1"
                     /translation="MRAADIFVSSGGEYQDIPNRRSFPSYSGDSIPQGLVRLSGVVLR
                     RDVSAEGNVSLYLFLKETGPVWVSAPGASRGRVRFGGAIEPLVWANFNLYKGARRFYL
                     QSAEVKEDFWSLRSDSGKIRRMLEWDGLLCEHLVPGLPCDDVLALFYWSGVLLKEDVH
                     PLVAEWRFLWKWLYNWGIAPSPDLCGECGAALSSARLTASGFLCPGCARTHEGTALSG
                     DELSLLRRSLEIPVKKMIEYFPSKPFPGDSPWKEVNGRLRLYFSMMQ"
     gene            1451..2338
                     /locus_tag="P5201_06805"
     CDS             1451..2338
                     /locus_tag="P5201_06805"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008710280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004820 - glycine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006426 - glycyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit alpha"
                     /protein_id="HRV98280.1"
                     /translation="MNFQEIVFRLERFWAEQGCVVQQPYDIEVGAGTMNPATCLRVLG
                     PEPWRVAYVEPSRRPTDGRYGENPNRLQHYYQYQVIIKPAPSNIIDLYLQSLAALGIE
                     PEAHDIRFVEDDWESPTVGAWGLGWEVWLDGMEVTQFTYFQQVGGIDMDLVPAEITYG
                     IERIAMFVQKVENVYDLQWVDNVKYGDVHHKGEVEYSHYNFELADTKMLFQLFGMYEA
                     EADRVLAKGFVLPAYDYVLKCSHTFNLLDARNAISVTERTGYIGRIRMLASRCCAAYA
                     EQRREMGLPFTGKFDETKR"
     gene            2344..>2930
                     /locus_tag="P5201_06810"
     CDS             2344..>2930
                     /locus_tag="P5201_06810"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013048159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004820 - glycine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006426 - glycyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit beta"
                     /protein_id="HRV98281.1"
                     /translation="MTYKNVLLEIGTEEIPSRFLPDTLEFLEKTAKDDFKNARIGFKS
                     LSVFATPRRIALMVKDVDDQQQDLVNTFKGPAWTAAFDMNGNPTRAAFGFAKSKGVSI
                     EQLVGVEVDGVKYAHAEVSEPGKPVLEILPELLPALIKKIVFPKNMYWNIPSVRFARP
                     IRWIVAMADKNVIPFQYGGIESGSATKGHRFMGAK"
BASE COUNT          617 a          854 c          884 g          575 t
ORIGIN      
        1 ggtacgcacc cccgtgcttc tcgtcgtgaa caagtgcgac gtcgttctcg accgaaaagg
       61 aggaggggag gcggcgcttg ccgtcgtgcg ctccctctac gaggcgcgaa tctctctctg
      121 cggggtcgtc ttcgtctccg ccagaatggg aagagggctc gatacgctta tcgccgcaat
      181 ccgtccccac cttccggagg ggcctccctt ctacgacgag gatatccttg tcgaccggcc
      241 cgagaagttc atcgccgcgg agatcatacg ggaaaaagtg ctgctgatga cgcaccggga
      301 ggtgccgcac agcgtcgccg tcgaaataga ggagtacaaa agccctgacg aataccccga
      361 gcgcgacgtg ctttttattc gcgccacgct ctatgtggag cgcgaagggc agaagcgcat
      421 cgtcatcggc gattccggag cgcggctgaa ggagataggc agacgggcgc ggatggatat
      481 tgaaacgctc accggccaca aggtctatct tgacctctgg atcaaggtgc gcaaagactg
      541 gcgtcaatcc gacgccgatc tgcggatgct cggcattcag gaaaggtgac gggggtgcgt
      601 gcagcggata ttttcgtttc gtccggcggg gagtaccagg acattccgaa ccgccggtcc
      661 ttcccgtcct atagcggcga ttccatcccc caggggctcg tcagactgtc cggggttgtg
      721 ctccgcaggg acgtctccgc agagggaaac gtctctctct atctctttct gaaggagacg
      781 gggccggtct gggtctctgc gccgggagcg tcgagaggga gagtccgttt cggcggcgcc
      841 atcgagccgc tcgtctgggc gaatttcaac ctgtacaagg gggcgcgccg cttctacctt
      901 cagtccgcgg aggtaaaaga ggacttctgg agcctgaggt ccgattccgg aaaaatccgg
      961 aggatgctcg aatgggacgg actgctctgc gaacacctcg tacccggact cccctgcgac
     1021 gacgtgcttg cgctctttta ctggtcgggc gtgctgctga aagaagacgt ccatccgctt
     1081 gtcgcggagt ggcgcttcct gtggaagtgg ttgtacaatt ggggcatagc gccttcaccc
     1141 gatctttgcg gcgagtgcgg cgccgcgctc tcttcagccc gtctgacggc ttccggattt
     1201 ctctgcccgg gctgcgcccg tacgcacgaa gggacggcgc tttccgggga tgaactctcc
     1261 ttgctgcgtc ggtcgctgga aattccggtg aaaaagatga tagaatattt tccctcgaaa
     1321 ccgtttccgg gagactcgcc ctggaaggaa gtgaacgggc gtcttcgcct gtactttagt
     1381 atgatgcaat aatagtggaa aaagaataga gcttccggca gccggaagtt tttctcaagg
     1441 agggatatat gtgaatttcc aggaaattgt ctttcgcctc gaacgcttct gggcggagca
     1501 ggggtgtgtt gtacagcagc cgtacgacat agaggtcggg gccgggacta tgaatcccgc
     1561 gacctgcctt cgcgtcctcg ggcccgaacc gtggcgcgtc gcctacgtcg aaccctcgcg
     1621 ccgtccgacc gacggccgct acggagagaa cccgaaccgg cttcagcact actaccagta
     1681 tcaggtgatc atcaagcccg ccccctccaa catcatcgat ctctaccttc agagcctcgc
     1741 ggctctcggg atagaaccgg aggcgcacga tatccggttt gtcgaggacg actgggagtc
     1801 gcccaccgtc ggcgcgtggg ggctcgggtg ggaggtctgg ctcgacggca tggaggtcac
     1861 gcagttcacc tacttccagc aggtcggcgg catcgacatg gaccttgtcc ccgcggagat
     1921 aacctacggg atcgaacgca tcgccatgtt cgtccagaag gtcgaaaacg tctacgatct
     1981 ccagtgggtg gacaacgtga agtacggcga cgtccaccat aagggcgagg tcgagtactc
     2041 gcactacaac ttcgaacttg ccgacacgaa gatgctcttc cagctcttcg gcatgtacga
     2101 ggccgaagcc gacagggtgc ttgcaaaagg attcgtcctt cccgcgtacg actatgtgct
     2161 caagtgttcg cacactttca acctgctcga cgcgcgcaac gcgatcagcg tgaccgagag
     2221 gacggggtac atcggaagaa tacgcatgct cgcaagcaga tgctgcgcgg cctacgccga
     2281 gcagcgccgg gagatggggc ttcccttcac cggcaaattc gacgagacga agaggtgagg
     2341 accatgacct ataaaaacgt actgctcgaa atcgggaccg aggaaattcc ctcacgtttc
     2401 ctccccgata cgctcgaatt cctcgaaaaa acggcgaagg acgacttcaa gaacgcccgc
     2461 ataggcttca agagtctctc cgtctttgcg acgccgcgcc gaattgcctt aatggtgaaa
     2521 gacgtcgacg atcagcagca ggacctggtg aacacgttca aaggaccggc atggacagcc
     2581 gcgttcgaca tgaacggaaa cccgacgcgc gcggccttcg gctttgccaa gagcaagggc
     2641 gtctccatcg aacagcttgt cggcgtcgag gtggacggcg tcaagtacgc ccacgccgaa
     2701 gtttcggaac ccggaaagcc ggtgctggaa atccttcccg aacttcttcc ggcgctcatt
     2761 aaaaaaatcg tctttccaaa gaatatgtac tggaacatac cctccgtccg ctttgcgcgc
     2821 cccatccgat ggattgtggc aatggccgat aaaaacgtca tcccgtttca gtacggagga
     2881 atcgagagcg gttcagcgac caagggacac cgcttcatgg gcgcaaagca
//
LOCUS       DAOTIL010000285         2927 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminobacteriaceae bacterium isolate AMR_MDS_3026
            NODE_40186_length_2927_cov_3.724234, whole genome shotgun sequence.
ACCESSION   DAOTIL010000285 DAOTIL010000000
VERSION     DAOTIL010000285.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868050
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminobacteriaceae bacterium (wastewater metagenome)
  ORGANISM  Aminobacteriaceae bacterium
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae.
REFERENCE   1  (bases 1 to 2927)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2927)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:19:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,224
            CDSs (total)                      :: 3,198
            Genes (coding)                    :: 3,184
            CDSs (with protein)               :: 3,184
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2927
                     /organism="Aminobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_40186_length_2927_cov_3.724234"
                     /isolate="AMR_MDS_3026"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:3034609"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..649)
                     /locus_tag="P5201_06815"
     CDS             complement(<1..649)
                     /locus_tag="P5201_06815"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRV98282.1"
                     /translation="MRKMDALWAYQEAEVAKASVEAEIRSTPARLKLNKLHKQLKTQQ
                     GVITKLGEDIETYEQQLNKLTDQVEKLEERLEVESGELVTIRQDEESTAEEMTELKRD
                     IERLSREIAQAVREAKSLATEIEQATAEYQKTIRAASVAKKEYDTLRVECEKERESRA
                     DELAKHDAKLESLGKQVDQTLLARYEQVKLHHAVPIARVVNSKCGGCNMSLPMVML"
     gene            complement(825..1709)
                     /locus_tag="P5201_06820"
     CDS             complement(825..1709)
                     /locus_tag="P5201_06820"
                     /inference="COORDINATES: protein motif:HMM:NF013250.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016780 - phosphotransferase activity, for
                     other substituted phosphate groups [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-alcohol phosphatidyltransferase family
                     protein"
                     /protein_id="HRV98283.1"
                     /translation="MTGQQVHKLRILANWRRIVPTMVTLSALFFGFASILSTIEAMRL
                     DVPALLGRSAQYIMLALILDGLDGNVARWLKGTTEFGAELDTFVDFLAFGVAPAALLY
                     VMLIHGPSPFWRTILPSAIVFSGALRLSRFRLKDPLRGQGGFIGLPITVNSSWIALAA
                     FAAQIMYPEGHILVTGPVSIVFQFLILVMISLQLSNLRYPKPSNKVRFFAPAVLAVLL
                     LWALSETYAAWIATALVFGCLTYVLTGPMIHKRSALRQPGSACTPRCRDGRQRRKLHS
                     PRREGAGRCFTHGRNQAF"
     gene            complement(1713..2390)
                     /locus_tag="P5201_06825"
     CDS             complement(1713..2390)
                     /locus_tag="P5201_06825"
                     /inference="COORDINATES: protein motif:HMM:NF014696.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004609 - phosphatidylserine decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylserine decarboxylase"
                     /protein_id="HRV98284.1"
                     /translation="MKFRPEVKPFLLASFVFGVFIYTIAKVSGASNGLAFFLGLLFGG
                     AGTGYMLFFFRDPEIVTPAEPGIVVAAANGKLAKIVEMYEKDYLKQDTIRISIFLSLF
                     DVHVNRFPIGGFSTFLGYFPGKRLFTFDEKSSDVNQHNAILVRNEETSCLIRQIVGPV
                     CRRVVYWLREDKTTTVKQGDRFGMMKFGSRLDMYFPKDDIEMVAKIGDQVTAGETIIA
                     RIVKKGK"
     gene            complement(2436..>2927)
                     /locus_tag="P5201_06830"
     CDS             complement(2436..>2927)
                     /locus_tag="P5201_06830"
                     /inference="COORDINATES: protein motif:HMM:NF014069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HRV98285.1"
                     /translation="GREAGLHSFGESAKELLFYAALLHDIGLFLSFSNHHAHSHYIIR
                     NVELLGFHDNEIAVIAALAFYHRKRSPKKKDPEFEALDQDSQRIVFTLSMFLRMAESL
                     DRSHHNFVHEVRFVREKNVLLLKIQADKGYQLELWALEEHQKYFVKTFGERFGIQVTA
                     SGF"
BASE COUNT          587 a          820 c          872 g          648 t
ORIGIN      
        1 tgagcattac catcggcagc gacatgttgc atccgccgca tttgctgttg accacccgcg
       61 caatcggcac ggcgtggtgc agttttacct gctcgtacct ggccagaagc gtctggtcaa
      121 cctgctttcc gagggattcc agctttgcgt cgtgctttgc cagctcgtcc gcgcggcttt
      181 cgcgctcttt ttcgcactcc acgcggagcg tatcgtattc tttcttggcg acgcttgccg
      241 cacggattgt tttctggtac tcggctgtcg cctgttcaat ttcggttgcc agcgatttgg
      301 cttcccgtac ggcctgtgct atctcgcggc tgaggcgttc gatgtcccgc ttgagttcgg
      361 tcatctcctc ggcggtgctc tcttcatcct gacggatggt tacgagctct ccgctttcga
      421 cttccagccg ctcttcgagt ttttctacct gatcggtcag cttattgagc tgctgctcgt
      481 aggtttcgat gtcctcgcca agtttcgtta taacaccctg ctgcgttttt aactgcttat
      541 gcagtttatt gagttttaat ctcgccggcg tagagcggat ttcggcttct acggaagcct
      601 tggccacttc tgcctcctga tatgcccaaa gggcatccat ctttctcacg gcatttccct
      661 cctaaaggcg cacgaatgga ttcacgtccg cgcgcgataa attatattgt acatcaaaac
      721 attcgttctg taaacgccga atcagcggcg caagcaccac gcgctcggtt tcgtaatgcc
      781 cggcaatcac cagattaagg ccgaacacct gcgcgtccag cgcgtcagaa tgcttgattt
      841 ctcccgtgag taaaacatct gcccgctcct tcgcggcggg gtgaatggag cttcctccgc
      901 tgccgcccat cacggcaacg cggcgtacaa gcgcttccgg gctgccgcag agccgaacgc
      961 ttgtggatca tcgggcccgt gagcacgtag gtgaggcatc caaagaccag ggcggtggca
     1021 atccacgcgg catacgtctc gctgagcgcc cacagaagaa gaacggcgag aaccgccggg
     1081 gcgaagaagc gcaccttatt ggacggcttg gggtagcgca ggttggagag ctgcagcgag
     1141 atcatcacca ggatgaggaa ctggaagacg atggagaccg ggccggtgac aaggatgtgc
     1201 ccctcggggt acataatctg cgcggcgaag gccgcgagcg cgatccacga agagttcacc
     1261 gtgatgggaa ggccgatgaa gccgccctgg ccgcgcagag gatccttcag ccggaagcgc
     1321 gaaaggcgga gcgcgccgga gaagacgatg gcagagggaa gaatggtgcg ccagaacggg
     1381 ctggggccgt gaatgagcat gacgtagagc agcgctgccg gagccacgcc gaaggcgagg
     1441 aagtcgacga aggtatccag ctccgcgccg aactcggtcg tccctttcag ccagcgggcg
     1501 acgttgccgt cgagcccgtc gagaatgagc gcgagcatga tgtactgcgc ggaacgccca
     1561 agcagcgcgg gaacatcgag gcgcatcgcc tcgatggtgg agagtatgct ggcgaagccg
     1621 aagaagagcg cggagagcgt caccatggtc gggacgatcc gtctccagtt ggcgagaatt
     1681 ctcaatttat gcacttgctg tcctgtcatt gtttactttc ctttcttcac tatccgggcg
     1741 atgatcgtct cgcccgcggt gacctggtcg ccgatcttcg cgaccatctc gatgtcgtcc
     1801 ttggggaagt acatatcaag gcgcgagccg aacttcatca tgccgaaacg gtcgccctgc
     1861 tttacggtgg tcgtcttgtc ctccctgagc cagtagacga cgcggcggca gacggggccg
     1921 acgatttgcc gtatgagaca cgaggtttct tcgttgcgta ccagaatggc gttgtgctgg
     1981 ttcacgtccg acgacttctc gtcgaaggtg aagagccgtt tcccagggaa gtagccaagg
     2041 aaggtcgaga agccccctat cgggaagcgg ttcacatgga cgtcgaacag gctgaggaag
     2101 atgctgatgc ggatcgtgtc ctgtttcaga tagtccttct cgtacatttc aacgatcttc
     2161 gccagcttgc cgttcgcggc ggcgaccacg atgcccggtt ccgccggtgt gactatctcc
     2221 ggatcgcgga agaagaaaag catgtacccc gttcccgcgc cgccgaagag cagcccgagg
     2281 aagaaagcga gtccgttgga agcgcccgat accttggcta tcgtgtagat gaagacgcca
     2341 aacacgaacg acgccagaag aaacggtttc acttccggcc taaatttcat taaaaagccc
     2401 ccttgtgtgt atatggattc accttacatt ataccctaga aaccggatgc ggtcacctga
     2461 ataccgaaac gttctccaaa tgtcttgaca aaatactttt ggtgttcttc aagagcccag
     2521 agttcaagct gatacccctt gtcggcctgt attttgagca gaagcacgtt tttttcgcgg
     2581 acaaagcgaa cctcgtgcac gaaattgtgg tggcttcggt cgaggctctc ggccatccgg
     2641 aggaacatgc tgagggtgaa gacgatgcgc tgcgaatcct ggtcgagggc ttcgaactcg
     2701 gggtcttttt tcttcggcga gcgctttctg tggtagaagg cgagcgcagc gatgacggcg
     2761 atctcgttgt cgtggaagcc cagaagctcg acgttgcgga tgatgtagtg actgtgggcg
     2821 tggtggttgc tgaaggagag gaagagcccg atgtcgtgga gcagcgccgc gtagaagagc
     2881 agctccttcg cactctcgcc gaaggagtgc agcccggcct ctctccc
//
LOCUS       DAOTIL010000286         2919 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminobacteriaceae bacterium isolate AMR_MDS_3026
            NODE_40322_length_2919_cov_3.440642, whole genome shotgun sequence.
ACCESSION   DAOTIL010000286 DAOTIL010000000
VERSION     DAOTIL010000286.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868050
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminobacteriaceae bacterium (wastewater metagenome)
  ORGANISM  Aminobacteriaceae bacterium
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae.
REFERENCE   1  (bases 1 to 2919)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2919)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:19:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,224
            CDSs (total)                      :: 3,198
            Genes (coding)                    :: 3,184
            CDSs (with protein)               :: 3,184
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2919
                     /organism="Aminobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_40322_length_2919_cov_3.440642"
                     /isolate="AMR_MDS_3026"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:3034609"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..444
                     /locus_tag="P5201_06835"
     CDS             <1..444
                     /locus_tag="P5201_06835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006583588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent protease"
                     /protein_id="HRV98286.1"
                     /translation="DSASSTELYCLLSALSEVPLKQGIAVTGSVDQFGNIQPIGGVNE
                     KIEGFFEYCEHGGLTGEQGVLIPVQNTRHLMLDDTVIEAVSKGKFFIRAVSTIDEGIE
                     ALTGVSAESIHRKVKNKLKKWAKKTEKLKKGKDSEAGGDDDSEEE"
     gene            425..1129
                     /locus_tag="P5201_06840"
     CDS             425..1129
                     /locus_tag="P5201_06840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005662244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease III"
                     /protein_id="HRV98287.1"
                     /translation="MTPKKNSKKEPPSRPQDIDTVFDILEELWGNEKNEPRLGHEEPL
                     DGLILTLLSQNTNDRNRDKGFAALKNSFPSWELVASASAEAIADRIRPAGLAKTKSER
                     MLQILAALKERFGEYSLMALKDMDNDAVREYLSSLPGIGAKTVACVMLFDLGMSAFPV
                     DTHIARFCRRMKWAAESLPPDEIQPLLEEWVPERRFLGGHVNIIEHGRGLCHARTPEC
                     GKCPLQSNNLCPSVQE"
     gene            complement(1199..1528)
                     /locus_tag="P5201_06845"
     CDS             complement(1199..1528)
                     /locus_tag="P5201_06845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006583586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YerC/YecD family TrpR-related protein"
                     /protein_id="HRV98288.1"
                     /translation="MTEKWKDKLTDQLCRALLSLETPDEVYCFLEDVATIGEIRALAQ
                     RLEVARLLSEAYTYPQIAQQTGASTATISRVKKFLEYGADGYKIVLDRLKEAGEATNP
                     EDKSSKA"
     gene            complement(1535..2554)
                     /locus_tag="P5201_06850"
     CDS             complement(1535..2554)
                     /locus_tag="P5201_06850"
                     /EC_number="2.6.1.9"
                     /inference="COORDINATES: protein motif:HMM:NF012382.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004400 - histidinol-phosphate
                     transaminase activity [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol-phosphate transaminase"
                     /protein_id="HRV98289.1"
                     /translation="MRRLLDKNENPFDLPEEIKALILQKIGRASFNRYPDPEYAVLKE
                     RLSMYCGFSADSIVPGNGGDEILWMLFSRYVKPGDSVLVFSPTFSEYYRLADLFGASL
                     CTVQADLNGDEPRFDHTLFLSRMAELKPSLVLVDSPNNPTGQSHPVSFIGKAVALCES
                     TIVIDEAYGEFARDNWLSSMRGKVLPANVVVLKTLSKAWGLAGLRLGYAVCGKDSASA
                     LNAARSPFNVNILSGTAAEIILEHSEVMLARVKLLCGIRDGFISLVNRLQGWRAFSSD
                     ANFVLVRAPFPWSSFASSLGDLCLKRFALSSDTEDSTCWLRVSIGTEEDMESVISLFA
                     TMQRR"
BASE COUNT          794 a          735 c          740 g          650 t
ORIGIN      
        1 gacagcgctt cctcgacgga gctttactgc ctcctctcgg ccctctccga ggttccgctc
       61 aagcagggga tagcggtaac cggatcggtt gaccagttcg ggaacatcca gcccatcggc
      121 ggggtcaacg aaaagatcga aggtttcttc gagtactgcg aacacggcgg tctcaccgga
      181 gagcaggggg tactcatccc cgtgcagaac acacggcatc tcatgctcga cgacacggtg
      241 atcgaggcag tcagcaaagg gaaattcttc atccgcgcag tctcgacgat cgacgaaggg
      301 attgaggcgc ttaccggagt ttccgcagaa tcgattcatc gcaaggtaaa gaacaagcta
      361 aaaaaatggg cgaagaagac ggaaaaactc aaaaaaggca aagactccga ggcaggagga
      421 gacgatgact ccgaagaaga atagcaagaa agagccccca tccaggccgc aagatatcga
      481 cacggttttc gacatactgg aagagctgtg gggaaatgaa aagaacgagc cccgcctggg
      541 acatgaagaa cccttagacg ggctcattct cacacttctt tcccaaaaca cgaatgatcg
      601 caacagggat aagggcttcg ccgcactgaa aaattcgttc ccttcttggg aacttgtggc
      661 atcggcatcg gctgaagcta ttgccgatcg aatccgcccg gctggtcttg caaagacaaa
      721 atcggagaga atgctccaga tactcgcggc actcaaggaa agattcggcg agtattcgct
      781 catggcattg aaagacatgg acaacgatgc agtacgagag tatctttcct cgctccccgg
      841 gataggggcg aagaccgtgg cgtgtgtgat gctcttcgac ttagggatgt ctgcctttcc
      901 ggtcgacacg catatcgcca ggttctgccg gagaatgaag tgggcggcgg aatcactccc
      961 tcccgacgag atacagcctt tgcttgaaga atgggttcca gagagacgtt ttctgggagg
     1021 acatgtcaac attattgaac acggaagagg gctgtgtcac gcccgtaccc ccgagtgtgg
     1081 gaagtgcccg ctgcagagca acaacctatg cccgtcggtt caagaataaa caaatgtaaa
     1141 tcagcagagg agagcatttt tcgagctccc tctgctggtt tagaaaagag aagaaggatc
     1201 aggccttgga agatttgtct tcgggattcg tcgcttcacc ggcctctttc aaacgatcaa
     1261 gcacaatttt gtacccgtcg gcgccgtact caaggaactt ttttactctg ctgatcgtcg
     1321 cagtgcttgc tcccgtttgc tgcgcaattt gcggataggt gtacgcctcg ctcaacaacc
     1381 gggcaacttc gagccgctga gcaagagcgc ggatctcgcc aatggtagcg acatcttcaa
     1441 ggaaacagta gacctcatcc ggcgtttcta gagagaggag ggcgcggcac agctggtctg
     1501 tcaacttatc cttccatttt tcagtcatag cattctacct cctttgcatt gtggcgaaaa
     1561 gagaaatgac ggactccata tcttcctctg ttccaatact tacccgtaac cagcatgttg
     1621 agtcctctgt atcggacgaa agagcgaagc gcttcaagca caaatctcca agagaagaag
     1681 caaaactgct ccagggaaaa ggagctcgga caagtacaaa attcgcgtcg ctcgaaaagg
     1741 ctctccaccc ctggaggcga ttaacaagcg atataaatcc gtcccggatg ccgcaaagga
     1801 gtttaactct tgcgagcatc acttctgaat gctccagaat aatttcggcg gcagttcccg
     1861 aaagaatatt tacgttgaac ggtgatcggg cagcattcag cgctgaagcc gaatcttttc
     1921 cgcaaacagc gtacccaagc cggagccccg caagccccca cgctttggaa agcgttttca
     1981 atacaacgac gtttgcaggc aatacctttc ctcgcatcga tgaaagccag ttatcccgcg
     2041 cgaactcccc gtacgcctcg tcgatcacaa tggttgattc acaaagcgca actgccttgc
     2101 cgataaagga aacggggtgt gattggcccg tcgggttgtt cggagaatcc acgaggacca
     2161 gcgaaggttt cagctccgcc atcctgctta aaaaaagcgt atgatcgaag cgcggttcgt
     2221 caccgtttaa gtctgcttga acggtacata aactggctcc gaaaagatct gcgaggcgat
     2281 agtactctga aaaagtaggt gaaaatacga gcacggaatc tcctggtttg acataacggg
     2341 aaaaaagcat ccagagaatt tcatcgccgc cgtttccggg aactatagag tcagcggaga
     2401 aaccacaata catggagagt ctctctttta aaactgcata ctccgggtcc ggatatctat
     2461 taaaggacgc gcgcccgatt ttttgcagga taagcgcctt aatctcttca gggaggtcga
     2521 acggattttc gtttttatca agcaaacggc gcaatgggtt caccgcctcc ttctattttc
     2581 agcaagagaa tctttgaact acgacgtaca gaggagaata ccatatcaat ttccaggaac
     2641 atctgtacta tgccatctgc ttcaaaacac aaaaactctt tctatgctcg aagcgcactt
     2701 ggactttagc atgttacaat gtatttattt ggcagtctag cattctaccg cgtcaatgtc
     2761 aacgataagt gcgcgttgtg tgcgttgcca gttcatgttt cgcactgcgc atcaaggagg
     2821 gaaaaaattg aactccatcg aaatgattgt cgtcgaacgt ctttttgaag aagaagggat
     2881 tcttgagcaa tacaactatg tcacagtgat aggaaatta
//
LOCUS       DAOTIL010000287         2896 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminobacteriaceae bacterium isolate AMR_MDS_3026
            NODE_40722_length_2896_cov_3.504752, whole genome shotgun sequence.
ACCESSION   DAOTIL010000287 DAOTIL010000000
VERSION     DAOTIL010000287.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868050
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminobacteriaceae bacterium (wastewater metagenome)
  ORGANISM  Aminobacteriaceae bacterium
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae.
REFERENCE   1  (bases 1 to 2896)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2896)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:19:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,224
            CDSs (total)                      :: 3,198
            Genes (coding)                    :: 3,184
            CDSs (with protein)               :: 3,184
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2896
                     /organism="Aminobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_40722_length_2896_cov_3.504752"
                     /isolate="AMR_MDS_3026"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:3034609"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..542
                     /locus_tag="P5201_06855"
     CDS             <1..542
                     /locus_tag="P5201_06855"
                     /inference="COORDINATES: protein motif:HMM:NF014069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="HD domain-containing phosphohydrolase"
                     /protein_id="HRV98290.1"
                     /translation="HAASLHDIGKVGIPDAVLLKEGELTEVELETIRRHPGIGAATLE
                     RVRHEHPGNEFLTMGIELTRFHHEKWDGSGYPDGLAGETIPLSARIMALVDVYDALRS
                     KRPYKDAFSHEQACALINKGTGTHFDPSVAEAFAAAEAEFANVYERLQQRREHAEKTP
                     FAEACAEKRNDDEQRSASE"
     gene            562..1719
                     /locus_tag="P5201_06860"
     CDS             562..1719
                     /locus_tag="P5201_06860"
                     /inference="COORDINATES: protein motif:HMM:NF012678.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030554 - adenyl nucleotide binding
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase"
                     /protein_id="HRV98291.1"
                     /translation="MPTPFECAQPRRVLYGRGRLREAGHIVREYGKRCLVCTGSRPER
                     AAELLDSLRGAGLVCELLTVSGEPSFESIRQATARAAEFSPDAVVAMGGGSVLDTGKA
                     LAMLATNGGDPLDYAEIVGAGKPIMKTSLPLLAIPATAGTGSEATRNAVLAHTEKQVK
                     VSLRSRSMMPETVILDPHAMNGLPAHIIAASGMDALCQLIEAFVSCKANAWTDALCRA
                     GIPMAFRALPKACDDPDDLGAREEMLLAACWSGVALANAGLGAVHGFAGVVGGLTGAP
                     HGLICATLLAPVCRANIAALGPEGAHPALGKYAELAEMLTGRDAEPEEGVGELERLAA
                     RLPLRPFHVPEDTDGERVVAGAMAASSMKGNPVVLPKETLAALWRSVTKTS"
     gene            1716..2756
                     /locus_tag="P5201_06865"
     CDS             1716..2756
                     /locus_tag="P5201_06865"
                     /inference="COORDINATES: protein motif:HMM:NF013862.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HRV98292.1"
                     /translation="MTRTNPSAASKQTEKRPRLGLALGAGAARGMAHLGVLRELEEAG
                     LAPDVVAGTSIGALVGGMYAAGKLDRLTEVALGLDWKQIARYFVEISFPRSGLIEGNK
                     LTALLAEIVGEVELTELALPFRAVTTNLMTGEEVVISDGDLVEAIRASIAVPGIFTPA
                     RRGDDLLVDGGLVNPVPVSVCRTMGAERIIAVDLNYGRVKDTRARNSTVKEKSALASQ
                     RPNFDAGQIFEWLERNTKQFDIELPNPLKSWMERHALPNVFDVLGNTLGIIESQIAAT
                     RLQVDKPDLLIQPNVGDIRFMDFVHAGEMIEEGRRAAKEALGQWKENGSELPDARSPK
                     NASRPKEERRGV"
BASE COUNT          566 a          920 c          983 g          427 t
ORIGIN      
        1 accacgcggc gtcgctgcac gacatcggca aggtaggcat ccccgacgcc gtcctcctga
       61 aagagggcga gctcaccgaa gtagagctgg aaacgatacg acggcacccc ggaatcgggg
      121 cggcgacgct ggagcgtgtg cggcacgagc accccgggaa cgagtttctc accatgggaa
      181 tagagctgac acgcttccat cacgaaaaat gggacggcag cggctacccc gacggcctcg
      241 cgggcgagac cattcccctg tccgcgcgca tcatggcgct ggtggacgtc tacgacgcgc
      301 tccgctccaa gcgtccgtac aaggacgcct tctcccacga gcaggcgtgc gcgctcatca
      361 acaaaggtac gggaacgcac ttcgacccgt ccgtcgcgga ggcgttcgcc gccgccgaag
      421 cggagttcgc caacgtctac gaacgcttgc agcaaaggcg agaacatgcc gaaaaaacgc
      481 ctttcgctga agcatgtgca gaaaagagga atgacgatga acaacggagc gcaagtgaat
      541 aaaatacagg gaggccggga aatgccgacg ccgttcgagt gcgcgcaacc gcgcagggtg
      601 ctttacggga gaggccgtct ccgggaggcg gggcatatcg tccgcgagta cggcaaacgc
      661 tgcctcgtct gcaccggaag ccgtccggag cgggcggcgg aactgctcga ctccctgcgc
      721 ggggccgggc tcgtctgcga gctgctgacc gtgtcgggcg aacccagctt tgagagtatt
      781 cgtcaggcga ccgcccgcgc tgcggaattc tcccccgacg cagtggtcgc catgggcgga
      841 gggagcgtgc tcgacaccgg caaggcgctg gccatgctgg cgaccaacgg cggcgatccg
      901 ctcgactacg cggagatcgt cggcgcgggg aagcccataa tgaaaacctc cctcccgctg
      961 ctggcgatcc cggcgacggc ggggacaggg tccgaggcta cccgcaacgc ggtgctcgcg
     1021 cacaccgaaa aacaggtcaa ggtgagcctg cgcagccgct ccatgatgcc ggagaccgtc
     1081 atcctcgacc cgcacgccat gaacggcctg ccggcccaca tcatcgccgc gagcggaatg
     1141 gacgcgctct gccagctgat cgaggcgttc gtctcgtgca aggcgaacgc ctggaccgac
     1201 gccctctgcc gcgcgggaat ccctatggcc ttccgcgcgc tgccgaaggc gtgcgacgac
     1261 cccgacgacc tcggcgcacg cgaggagatg ctgctggccg cgtgctggag cggcgtcgcc
     1321 ctcgccaacg cgggactggg agcggtccac ggcttcgcgg gagtggtcgg aggactgacc
     1381 ggcgcgccgc acggactgat ctgcgccacg ctgctcgcgc ccgtctgccg ggcgaacatc
     1441 gccgcgctcg gcccggaggg agcgcacccg gcgctcggga agtacgccga gctggcggaa
     1501 atgctcacgg gacgcgacgc ggaaccggaa gagggagtcg gcgaactcga acgcctcgct
     1561 gcgcgcctgc cgctccggcc gttccacgtt cccgaagaca cggacggtga gcgtgtcgtc
     1621 gcaggggcca tggcggcgag cagcatgaag ggcaatccgg tcgtcctgcc gaaggagacg
     1681 cttgcggctc tctggaggag tgtgacgaag acatcatgac gaggacaaac ccatcggcgg
     1741 cctcaaagca gaccgaaaaa cgtccgcgcc tcgggctcgc actgggcgcg ggggcggcgc
     1801 ggggcatggc gcacctgggc gtcctccgcg agctcgaaga ggccggactc gctcccgacg
     1861 tcgtcgccgg gacgagcatc ggcgcgctgg tcggagggat gtacgcggcg gggaaactcg
     1921 accgcctgac cgaggtcgcg ctcgggctgg actggaagca gatcgcccgc tacttcgtcg
     1981 aaatatcctt tccgcgctcc gggctgatcg aggggaacaa gctcacggcg ctcctcgcgg
     2041 agattgtcgg cgaagtcgaa ttgacggagc ttgcgctccc cttccgggcc gtgaccacga
     2101 acctcatgac gggcgaagaa gtcgtcatca gcgacggcga ccttgtggag gcgatacgcg
     2161 cgagcatcgc ggttccgggc atctttactc cggcgcgcag gggcgacgat ctgctggtcg
     2221 acggcgggct ggtgaatccg gtgccggtga gcgtctgccg tacaatgggc gccgagcgca
     2281 tcatcgcggt ggatctgaac tacggccgcg tcaaggatac ccgcgccaga aattcgacgg
     2341 tcaaagaaaa gagcgcgctc gcttcgcaac gtccgaactt cgacgccgga cagatcttcg
     2401 agtggctgga gcgaaatacg aagcagttcg acatcgagct tcccaacccg ctgaagtcat
     2461 ggatggaacg gcacgctctg ccgaatgtct tcgacgtgct gggaaacacg ctcggcatca
     2521 tcgaatcgca gatagcggcg accagattgc aggtcgacaa accagacctg ctgatccagc
     2581 ccaacgtcgg cgacatccgc ttcatggact tcgtccacgc gggcgagatg atcgaggagg
     2641 gacgccgtgc ggcgaaggaa gcgctcggac agtggaagga aaatggaagt gaacttcccg
     2701 acgcacgttc gccaaagaac gcttccaggc caaaggagga gcggaggggc gtatgataca
     2761 ggagagcgtg agaaccctgg ccgatttttt ctccttcgac gctctcgtcg gcatccatgt
     2821 tttgaggatt ctctacttcg ccggggccgt cggaatccct ctcatcggct ggtttttcgc
     2881 ccggaggata cgggcg
//
LOCUS       DAOTIL010000288         2886 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminobacteriaceae bacterium isolate AMR_MDS_3026
            NODE_40922_length_2886_cov_2.887672, whole genome shotgun sequence.
ACCESSION   DAOTIL010000288 DAOTIL010000000
VERSION     DAOTIL010000288.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868050
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminobacteriaceae bacterium (wastewater metagenome)
  ORGANISM  Aminobacteriaceae bacterium
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae.
REFERENCE   1  (bases 1 to 2886)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2886)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:19:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,224
            CDSs (total)                      :: 3,198
            Genes (coding)                    :: 3,184
            CDSs (with protein)               :: 3,184
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2886
                     /organism="Aminobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_40922_length_2886_cov_2.887672"
                     /isolate="AMR_MDS_3026"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:3034609"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..284
                     /locus_tag="P5201_06870"
     CDS             <1..284
                     /locus_tag="P5201_06870"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRV98293.1"
                     /translation="GWGRMKASQETGLSKLREDSRGIEAAEVFPTHSPETRRGAALEE
                     DHWEGPFPVWNGPFYYPRQLHGGVRSFPPGAHGVRGAFSRLSLHGRGKG"
     gene            281..1174
                     /locus_tag="P5201_06875"
     CDS             281..1174
                     /locus_tag="P5201_06875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015556836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016852 - sirohydrochlorin
                     cobaltochelatase activity [Evidence IEA];
                     GO_process: GO:0019251 - anaerobic cobalamin biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sirohydrochlorin cobaltochelatase"
                     /protein_id="HRV98294.1"
                     /translation="MRKMSCAVFVFVVCLFGLLSASAFASEHAKPEGKQGILLVAFGT
                     SEPDARSAIHGIVDAVRKAFPNAEVRLSYTSNIIRRKILKEEGLVVDTPPISLAKMQD
                     EGFTSVVVQSLHIISGEEFHQLAGIVRAFSSIRGKYGFERLSLGRPLLDSMEDYRKTV
                     DMLKAKYASFAADGGVVTFMGHGTHHAANAAYSKMQLIFDEEGLPFVMGLVEAFPDIE
                     SVKRRLKELKPSRVTLVPFMVVAGDHAKNDMADTEDPESWVSVLASEGYAVEPLLKGL
                     GDGEGLAELFIGHIREAMEDR"
     gene            1244..2236
                     /locus_tag="P5201_06880"
                     /pseudo
     CDS             1244..2236
                     /locus_tag="P5201_06880"
                     /inference="COORDINATES: protein motif:HMM:NF013219.2"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006826 - iron ion transport [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
     gene            2233..>2886
                     /locus_tag="P5201_06885"
     CDS             2233..>2886
                     /locus_tag="P5201_06885"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HRV98295.1"
                     /translation="MRGSLRVSGGGIQLDCVSAGYGERRVLDGLSAEFPSGALTMLLG
                     PNGSGKSTLLRLLGGALPFSGNAVLAGRSLRRLSPGQRGKLVGMVSQSPSLTFPFTVE
                     EVVLLGRLPHRKFLSGWTMEDRGCAMEAACEMELGEMLSRRVSSLSGGEKQRVLIAQV
                     IAQQPEVFLLDEPSSALDPRHTLRLFRFLRKCAREGATVVAAAHDINLAAEYADCVCF
                     "
BASE COUNT          435 a          838 c         1020 g          593 t
ORIGIN      
        1 aggggtgggg gaggatgaag gcgagccagg agaccggtct ttcgaaactg cgggaagact
       61 cgcgaggaat cgaggccgcc gaagtgtttc ccacgcattc cccggagact cgacggggag
      121 cggcgttgga agaagatcac tgggaggggc cgtttcctgt gtggaacggt cctttttatt
      181 atccccgcca actgcacggc ggtgtgcgct cgtttcctcc gggagcccat ggcgtccggg
      241 gagcattctc ccggctatca ctccacggga ggggtaaagg atgagaaaaa tgagttgtgc
      301 ggtctttgtg tttgttgtgt gtctgttcgg cctgctttcg gcaagcgcgt tcgcttcgga
      361 gcacgcgaag cccgagggga aacaggggat actgcttgtc gccttcggga cgtccgagcc
      421 cgacgcccgt tcggccatcc acggcatcgt cgatgcggtg cggaaggcct tcccgaacgc
      481 cgaggtccgg ctttcgtata cttcgaatat tatccgccgg aagatactca aggaggaggg
      541 gctcgtcgtc gacacgcctc cgatatcgtt ggcgaagatg caggacgaag gctttacgtc
      601 ggtcgtcgtg cagtcgctgc acatcatctc gggcgaggag tttcatcagc ttgccgggat
      661 agtgcgcgcc ttctcgtcca tacgcgggaa gtacggattc gaacgtcttt ccctcggcag
      721 acccctgctc gacagcatgg aggattacag aaagacggtg gacatgctga aggcgaagta
      781 cgcctccttt gcggccgacg gcggcgtagt gaccttcatg gggcacggta cgcaccacgc
      841 ggcgaatgcg gcgtacagca agatgcagct catcttcgac gaggaggggc tgcctttcgt
      901 catgggactc gtcgaggcct tcccggacat cgagagcgtg aaacggcggc tgaaggagct
      961 gaaaccttcg cgggtaacgc tcgttccctt catggtggtg gccggggatc acgcgaagaa
     1021 cgatatggcg gatacagagg acccggagtc ctgggtcagc gtgctcgcga gcgaagggta
     1081 tgccgtcgag cctcttttga aggggctcgg agacggcgaa gggcttgcgg agctctttat
     1141 cggccacata cgcgaggcta tggaggacag gtgagagcaa cgcgcggccg ggagctctcc
     1201 ggatttgtcc gggagcgccg ccccggagga gcggctattc agtatgcgac gttccggtga
     1261 acgttcgcgg acgtactgtg ttttcccggg gctgctcctc cttctgggag gagcggccct
     1321 gtggcgtcta ctctccggag acatggcaat acccctttcc ggcgcgctca aggctgttct
     1381 tggattggaa ggcgttctta ccccgcagca ggcgctcgtc atacaggcgg tccggctgcc
     1441 gcggcttctc gcgtcgctgg gagcgggcgc ctcgctcgct gtgtccgggg tggtgctcca
     1501 ggggattctg gtgaacccgc tggcggagcc gtacacgctc ggcatcgcct cgggagcggc
     1561 gctcggcgct tcgctgggga tctccctcgg cggcgcatgg attccagctg ccgccttcgc
     1621 cggggcgctt gcagctcttt ttctttcgct cctccttgca tggcgttcgg gaggattctc
     1681 gccgctgcac atggtactct ccggcatcgt gatcggctcg gtgctctccg ccggggtgac
     1741 gctgctcaag gcccttgccg gggagcgggt ggcggcgatc gtcctctggc tgatgggaag
     1801 cttctcggga gcttcgatgg agggcgctct cgtcgtcgct gccgggggct tcgcgctctt
     1861 tgcggcggcg tggtggtgag ggcgcgacct cgacgcgatc tccctcggag agaacagggc
     1921 ggtctatctc ggggtggagg agcgccggat caagggaacg ctgctcgtgc tcgcttcgct
     1981 ggcaacggcg ctgacggtgg cttcgcacgg catcatcggc ttcgtggggc tcgtcgggcc
     2041 gcatctgttg cggatggctc tgggaccgtc gcaccgcatg ctgctgacgg cctccttcct
     2101 tggaggggcg cttttgctca ccgtggcgga cggggtcgcc cggtctctgg gcgagctgcc
     2161 cgtaggcgtg gtgacgtcgc tggccggagg gccggtcttc tgttggatac tgcttcgagg
     2221 gagggagaaa ctatgagggg atcgctacgc gtttccggtg ggggaattca gctagattgc
     2281 gtgtccgcag ggtacgggga gcgtcgtgtg ctcgacgggc tctccgccga gtttccgtcc
     2341 ggcgccctga cgatgctgct ggggccaaac ggcagcggca agagcacgct gttgcgtctg
     2401 ctggggggcg cgctcccgtt ctcagggaac gcggttctgg ccggccgttc gcttcgacgc
     2461 ctctctcccg gacagcgggg aaagcttgtc ggaatggtct cccagtcgcc gtcgctcacc
     2521 tttcccttca ccgtcgagga ggttgtgctc ctcggaaggc tccctcacag aaagtttctc
     2581 tcaggatgga cgatggagga cagagggtgc gcgatggagg ccgcgtgtga aatggagctc
     2641 ggtgagatgc tctcccgcag ggtgtcgtcg ctctccggag gggagaagca gcgggtgctt
     2701 atcgcgcagg tgatcgcgca gcagccggag gtcttccttc tcgacgaacc gtcttctgcg
     2761 ctcgaccccc ggcacacgct ccggcttttc cgcttcctcc gaaaatgcgc acgcgagggg
     2821 gcgacggtgg tcgcggcggc ccacgacatc aatctcgcgg cggagtacgc cgactgcgtc
     2881 tgtttc
//
LOCUS       DAOTIL010000289         2873 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminobacteriaceae bacterium isolate AMR_MDS_3026
            NODE_41183_length_2873_cov_3.111072, whole genome shotgun sequence.
ACCESSION   DAOTIL010000289 DAOTIL010000000
VERSION     DAOTIL010000289.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868050
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminobacteriaceae bacterium (wastewater metagenome)
  ORGANISM  Aminobacteriaceae bacterium
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae.
REFERENCE   1  (bases 1 to 2873)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2873)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:19:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,224
            CDSs (total)                      :: 3,198
            Genes (coding)                    :: 3,184
            CDSs (with protein)               :: 3,184
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2873
                     /organism="Aminobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_41183_length_2873_cov_3.111072"
                     /isolate="AMR_MDS_3026"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:3034609"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..846)
                     /locus_tag="P5201_06890"
     CDS             complement(<1..846)
                     /locus_tag="P5201_06890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014163354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proton-conducting transporter membrane subunit"
                     /protein_id="HRV98296.1"
                     /translation="MNWNDHLPALLLMIPLFGAFAAPLVCLLGKTMRNVLLVGLSFVT
                     LLVALLLWKSVLAGGIAVYVMGGESFNLTLPSGMALPIRIIMEVDAFSAFMVVCGSIA
                     SFAGALFSTNYMEKFTGLGRFVSLYFLLTLGMLGMMITGDFFNFFIFIEIASIASFGL
                     VAFWRDRPEAIEASLKYTLVSQVGSMIFLIAAGALYGKYNALNIAAIGSLLQFGVMEK
                     IALVFIIGTLAMKCGSFPMHMWLPDAYAEAPSGVSCLLVAVSQASLYGLMRVCFSLYG
                     INLGGS"
     gene            complement(843..1190)
                     /locus_tag="P5201_06895"
     CDS             complement(843..1190)
                     /locus_tag="P5201_06895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013048653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:proton antiporter"
                     /protein_id="HRV98297.1"
                     /translation="MIGNAPFFVVGLLFLGGLTAIFIEKNLIKIAIAIGIISSAVNLF
                     LVALGYRAGGTAPIHFLAGERTVMVLPTPQAMTLTAIVISLATTAFLLSLIMLVYRHY
                     GTLDVDEIRRLRG"
     gene            complement(1180..1647)
                     /locus_tag="P5201_06900"
     CDS             complement(1180..1647)
                     /locus_tag="P5201_06900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013048654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MnhB domain-containing protein"
                     /protein_id="HRV98298.1"
                     /translation="MNPLSVVVRTGCDLFAWFMVVFGVNVLVHGYMTPGGGFQGGAII
                     ATFTAFLLVAYGGRKLLGWANEKIFDLFEEVGLLAFFFLAFMGFPVTFFYNYLVKPLA
                     AGGVVPPAGTTALMSIAVGLEVSGALSMVILTMYRGIRMFETAPMEEEIGHDR"
     gene            complement(1644..1907)
                     /locus_tag="P5201_06905"
     CDS             complement(1644..1907)
                     /locus_tag="P5201_06905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013048655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRV98299.1"
                     /translation="MIFVVAALLLGAMVVGAVDNAHPFGYPGKTPMDDFFLADALPNR
                     STENIVTAIVFDYRGFDTIGEAAVLFTALCAISVLFREGGRKR"
     gene            complement(1913..2173)
                     /locus_tag="P5201_06910"
     CDS             complement(1913..2173)
                     /locus_tag="P5201_06910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013048656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4040 domain-containing protein"
                     /protein_id="HRV98300.1"
                     /translation="MTNALHISVLAVMLIAAFFALWFKDLISSVISLAVFSLMMALEF
                     YILQAPDVAIAEAAIGAALSTTIYIIALRACRRSKSEGGRKQ"
     gene            complement(2170..2544)
                     /gene="mnhG"
                     /locus_tag="P5201_06915"
     CDS             complement(2170..2544)
                     /gene="mnhG"
                     /locus_tag="P5201_06915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014163359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051139 - metal cation:proton antiporter
                     activity [Evidence IEA];
                     GO_process: GO:0098662 - inorganic cation transmembrane
                     transport [Evidence IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="monovalent cation/H(+) antiporter subunit G"
                     /protein_id="HRV98301.1"
                     /translation="MAFFILVIAALLVSVFFNSLGAVSLYRFPDVYTRLHGATKCTTF
                     GTIFIAVAVVLYAVGRLLESGENRFWVLIIHTLVAMMVLLVTNATGAHAIARASHRSG
                     LAPAKAVVDRLEESCSRGGASQ"
     gene            complement(2550..2810)
                     /locus_tag="P5201_06920"
     CDS             complement(2550..2810)
                     /locus_tag="P5201_06920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013048658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015075 - monoatomic ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0034220 - monoatomic ion transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="monovalent cation/H+ antiporter complex subunit
                     F"
                     /protein_id="HRV98302.1"
                     /translation="MTVHFVWFVAAVMGILAVAVLGRLISGPTVSDRAVALDTFNTFV
                     VGIMLLLAVGYDSVVMVDVAIVYAALSFVGTMFIARYIEGEV"
BASE COUNT          757 a          860 c          771 g          485 t
ORIGIN      
        1 ggatcccccg aggttgatgc cgtacaggga gaaacacacc ctcatcaggc cgtaaagcga
       61 ggcctggctc accgcgacca agaggcagga gacgcccgag ggcgcttccg cataggcatc
      121 gggaagccac atgtgcatcg ggaaggaacc gcacttcatc gcaagcgttc cgatgatgaa
      181 aaccaacgcg atcttctcca tgacgccgaa ttggagcaaa ctgcctatcg ctgctatgtt
      241 gagcgcgttg tacttgccgt acagagcgcc tgcggcgatc aggaagatca tcgaccccac
      301 ctgcgagaca agcgtgtatt taagacttgc ctcgatcgcc tccggtctgt cgcgccagaa
      361 tgcgaccaat ccgaacgagg cgatggatgc gatttcgatg aagatgaaga agttgaaaaa
      421 gtcgcccgta atcatcattc cgagcatacc gagcgtaaga aggaaataga gcgagacaaa
      481 ccgccccaag ccggtgaact tctccatgta gttcgttgag aagagggcgc cggcgaacga
      541 ggcgatcgaa ccgcagacga ccatgaaggc gctgaacgcg tccacctcca tgatgatgcg
      601 gatcggaagc gccataccgg agggaagcgt caggttgaag ctctcccctc ccattacata
      661 gaccgcgatt ccccccgcga gcacgctttt ccagagaaga agcgcgacaa gcagcgtcac
      721 gaaagaaaga ccgaccaaca gcacattgcg catcgtcttt cccaaaagac agaccaaagg
      781 cgcggcgaaa gcgccgaaga gaggaatcat caacaacagc gcgggaaggt gatcgttcca
      841 gttcatcctc tcaacctcct gatttcgtcc acgtcaagcg tgccgtaatg acggtagacc
      901 agcatgatga gcgagagaag aaaggccgtc gtcgcaagcg aaatcacgat cgcggtgagc
      961 gtcatcgcct ggggcgtcgg aagcaccatg accgtccgct cgcctgcgag aaaatgaata
     1021 ggcgccgttc ctccggcacg ataccccaaa gcgactagga acaggttgac agcggaactg
     1081 atgataccga ttgcgatggc gattttgatg agattctttt ctataaagat cgccgtcagt
     1141 ccgcccagga aaagaaggcc taccacaaaa aaaggagcgt tacctatcat gaccgatctc
     1201 ctcctccatc ggggcggtct cgaacatgcg gatccccctg tacatcgtga gaataaccat
     1261 cgaaagagcg ccgctcactt cgagtcccac cgcgatggac atgagcgccg tcgtgcctgc
     1321 cggcggaacg acgcctccgg ctgcaagggg cttcacaaga tagttgtaga agaacgtgac
     1381 ggggaatccc atgaaggcca gaaagaagaa agcaagcaac ccgacctctt cgaaaaggtc
     1441 gaatatcttc tcgttcgccc agcccagcag cttccgcccg ccgtaggcca ccaacaaaaa
     1501 ggcggtgaac gtcgcgataa tcgcccctcc ctggaagcct ccgccgggag tcatgtaccc
     1561 gtgcaccaga acgttgacgc cgaaaacgac cataaaccat gcgaacaggt cgcagcctgt
     1621 ccggactaca accgaaaggg gattcaccgt ttccttcctc cttccctgaa aagaaccgaa
     1681 attgcgcaga gcgcggtgaa taaaacagcg gcttcgccga tagtatcgaa tcctctgtaa
     1741 tcgaagacta tcgccgtgac gatgttctcg gtcgatctat tcggcagagc gtccgcaagg
     1801 aaaaaatcgt ccatcggagt tttccccggg tagccgaaag gatgcgcatt gtccacggcg
     1861 ccgacgacca ttgctccgag caaaagcgca gcgacaacga aaatcatctt tttcactgtt
     1921 tccgcccccc ttcactcttt gaacggcgac acgccctgag cgcgattata tagatcgtcg
     1981 tactcagagc cgctccgatg gccgcctcgg cgatcgcgac gtcgggcgct tgcagaatgt
     2041 agaattccag cgccatcatc agactgaaga ccgccaagga aatcaccgaa gagatgagat
     2101 ccttgaacca gagggcgaag aacgcggcaa tcagcataac cgccagaacc gagatgtgca
     2161 gcgcgttcgt cattgggacg cacctcctct ggaacagctc tcctcaagcc tgtcgacaac
     2221 ggcctttgca ggcgccaatc cgctccggtg agaggcccgc gcgatggcgt gcgctcccgt
     2281 tgcgttcgtg acaagcagca ccatcatcgc cacaagcgta tgtatgatga gcacccaaaa
     2341 ccggttctcc ccgctttcga ggagcctgcc gaccgcatag agcaccaccg cgaccgctat
     2401 gaaaatcgta ccgaacgtcg tgcacttcgt cgcgccgtga agcctagtgt acacgtcggg
     2461 gaatctgtag agggaaaccg cgccgagaga gttgaaaaaa acgctcacca acagagcggc
     2521 aatcaccaaa ataaaaaagg ccatccgggt tacacctccc cttcgatgta gcgggcgatg
     2581 aacatggtcc ccacgaagga gagcgcggcg tacactatgg ccacatcaac catgacgacc
     2641 gaatcgtacc cgacggcgag aagcagcatg atgcccacta cgaaagtgtt gaacgtgtcc
     2701 agagcaaccg ccctgtccga gaccgtcgga ccggagataa gacgcccgag gacggcgacg
     2761 gcgagaatgc ccataaccgc cgcaacaaac cacacaaaat ggaccgtcat tcagccaacc
     2821 tccttgccca gtgggcgaaa ttgccgtaca gctgttcttc cgagggattc tcg
//
LOCUS       DAOTIL010000290         2866 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminobacteriaceae bacterium isolate AMR_MDS_3026
            NODE_41329_length_2866_cov_3.556386, whole genome shotgun sequence.
ACCESSION   DAOTIL010000290 DAOTIL010000000
VERSION     DAOTIL010000290.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868050
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminobacteriaceae bacterium (wastewater metagenome)
  ORGANISM  Aminobacteriaceae bacterium
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae.
REFERENCE   1  (bases 1 to 2866)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2866)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:19:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,224
            CDSs (total)                      :: 3,198
            Genes (coding)                    :: 3,184
            CDSs (with protein)               :: 3,184
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2866
                     /organism="Aminobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_41329_length_2866_cov_3.556386"
                     /isolate="AMR_MDS_3026"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:3034609"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..424)
                     /locus_tag="P5201_06925"
     CDS             complement(<1..424)
                     /locus_tag="P5201_06925"
                     /inference="COORDINATES: protein motif:HMM:NF013116.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transaldolase family protein"
                     /protein_id="HRV98303.1"
                     /translation="MVQQNRILQALALGQSMWCDFLSRDLLRSGGLDRMIEQGVRGVT
                     TNPSIFENAIARTSDYDTDIQAMMREKKTEEEIYTALTIADVQEGADRFKTLFETSNG
                     VDGFVSLEVSPLLCHDEKGTVEEALRLFEQLDRKNVMIK"
     gene            complement(502..942)
                     /locus_tag="P5201_06930"
     CDS             complement(502..942)
                     /locus_tag="P5201_06930"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRV98304.1"
                     /translation="MDNVLRGSGTHFPRWMRWTGMGTGIFLLADGMRRLFAGGGDLSG
                     VFAYILIGTACIYIAGYEKKIFLSEKGFEREILFWGRGRKEQVLWMDMEELILVPSGK
                     GTAVLIPLKQRGWRAFFPGIREDDVRKFVARFREDLPVRKGSGS"
     gene            complement(945..2492)
                     /gene="nhaC"
                     /locus_tag="P5201_06935"
     CDS             complement(945..2492)
                     /gene="nhaC"
                     /locus_tag="P5201_06935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005659937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015385 - sodium:proton antiporter
                     activity [Evidence IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter NhaC"
                     /protein_id="HRV98305.1"
                     /translation="MEQTSAKTPTFGQALFVLLVDAAIISYGVLDIHFESGAVFGLAL
                     SAHIPLFLAAVFTAIMGAIFVGKPWREVEEGMINGITVALQAVLILLAVGGLVGAWIL
                     SGVVPTLIFYGLNLLSPQYFLLASLLICAIVSLSTGSSWSTSGTVGIALMGIGAGLGV
                     PAPVTAGFVISGAYFGDKISPLSDTTNLAPAVAGSELFTHIRAMLWTTGPTLIIVAGI
                     AYYMGSGYTGGEMDASRISIIQQLMSAEFSISMYGFLPPLLVLVLAAMKIPAIPGIVA
                     GMLLASVMALFQGISIPDVMSALYSGYSPSMIAEVAEAGDISSVNELLKGAAVFSADA
                     FESVKEVAGMLNGLLERGGLTGMLGTVALILVALVLGGIMETCGFLEVLLVRLMQSVK
                     SVFGLVASVLVSCVFTNMFLGDQYLALVMPGRMFKPAFEKFEQNGKKLDPSLLSRSLE
                     DSGTLTSVLVPWNTCGAYNSGVLGVPTLSYLPYAFLNYLNPLVALLMTRLGIGIPWIE
                     AGEKKAE"
     gene            complement(2673..>2866)
                     /locus_tag="P5201_06940"
     CDS             complement(2673..>2866)
                     /locus_tag="P5201_06940"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRV98306.1"
                     /translation="TKYGYHVFEVLERRRARALSFEEVRDAVRAELVEHVMATEAASL
                     KARFPVLLVPSALEKSFAR"
BASE COUNT          774 a          778 c          709 g          605 t
ORIGIN      
        1 tcttgatcat gacgtttttc ctgtcaagct gttcgaagag acgcaaggct tcttcaacag
       61 ttcctttttc atcgtgacag agcaaaggac ttacctcaag acttacaaaa ccgtcaaccc
      121 cgttgcttgt ctcgaaaagc gtcttgaaac ggtcggctcc ttcctgcaca tcggcgatgg
      181 tgagtgcagt atagatctcc tcttccgttt ttttctcgcg catcatcgct tgaatatccg
      241 tgtcgtaatc ggatgtcctg gcgattgcgt tctcgaagat ggagggattc gttgttactc
      301 cccgcactcc ttgttcgatc atcctgtcaa gacctccgga acggagcaga tctctgctca
      361 ggaagtcgca ccacatgctc tgtccaagcg ccagcgcctg caatatccta ttctgttgaa
      421 ccatggaatc tatcctttca tagagagtgg agaaattgga ggagacggga agtctcctcc
      481 tccgcgccgt tcacaatttt cttatgaacc gctccctttc cgaacgggaa ggtcttctct
      541 gaagcgagct acgaattttc tcacatcatc ctcgcgaatt cccgggaaaa aagccctcca
      601 ccctctctgt ttcaggggga taagaacggc ggttcccttc cccgatggaa cgaggatgag
      661 ttcctccata tccatccaga gaacctgttc tttgcggccc cgtccccaga aaagaatctc
      721 tctctcaaac cccttctctg agaggaagat cttcttttcg tatccggcaa tgtagataca
      781 cgctgtgccg atcagaatat aggcaaaaac acccgagaga tcgccgccgc cggcaaagag
      841 acggcgcatt ccgtctgcca ggagaaagat gcccgtcccc attccagtcc accgcatcca
      901 gcgggggaaa tgagttccgc tcccccgaag cacgttgtcc acgcctattc agccttcttt
      961 tcgccggctt caatccaagg tataccgata ccgagccttg tcatcaacag agcaacaaga
     1021 ggattcaggt aattcaggaa tgcgtacgga agatatgaca gagtgggcac gccaaggaca
     1081 ccgctattat aggcgccgca cgtattccac ggaacaagaa ccgaggtgag cgttccggaa
     1141 tcctcaagcg atctggagag caggctcgga tcaagttttt tcccgttctg ttcgaatttt
     1201 tcaaaggcgg gtttgaacat tcgtccgggc atgacaagcg caaggtattg gtcgccaagg
     1261 aacatgttag tgaagacgca cgatacaaga acagaagcga caagaccgaa caccgatttg
     1321 acgctctgca tcaagcgaac caaaaggact tcaaggaaac cgcacgtctc cataataccg
     1381 ccaagaacca aagcgacgag gatgagcgcc accgtcccaa gcatgccagt taaacctcct
     1441 ctttcgagaa gaccgttcag catgcctgca acctccttga cagattcgaa cgcatccgcg
     1501 gagaagaccg ccgctccttt aagaagctcg ttcacggaag agatatcccc ggcttcggcc
     1561 acctcggcta tcatggaggg agagtatccc gaatacagag cgctcataac atccggaata
     1621 ctgattccct ggaaaagcgc cataacggaa gccaagagca taccggcgac aatacccggg
     1681 atagcgggga tcttcatcgc cgcaaggacg aggacaagaa gcggaggaag gaaaccgtac
     1741 atcgagatgg agaactctgc agacatcaac tgctgaataa tcgagatcct ggaggcgtcc
     1801 atctcgcccc cagtgtaccc gcttcccata taataggcga ttccggcaac gatgatcagg
     1861 gtgggaccgg tagtccagag catcgccctg atatgggtaa aaagttccga accggctaca
     1921 gcaggagcaa ggttcgttgt atcggagaga ggagaaattt tatcgccgaa atacgcgccc
     1981 gagattacaa agcccgccgt tacaggagcg gggactccaa gccctgcgcc tattcccatg
     2041 agcgctattc ccacggttcc gctcgtgctc caggaacttc ccgtggaaag agagacaatg
     2101 gcgcagatca ggagagatgc caggaggaag tactgaggag acagaaggtt gagaccgtaa
     2161 aaaatgagcg tgggcacaac gccgctcaga atccatgccc caacaagacc tcccactgca
     2221 agcaatataa ggacggcctg aagcgcgaca gtaataccgt tgatcattcc ttcctcaact
     2281 tcacgccacg gtttaccgac aaaaatagct cccataatgg cggtaaagac tgcggcgagg
     2341 aagagcggaa tatgcgcgga gagggcgaga ccgaacaccg cccccgattc aaaatgaata
     2401 tcgagaaccc cataactgat aatagcagcg tccacaagaa gtacaaaaag agcttgacca
     2461 aaagtaggtg tctttgcaga tgtttgttcc atacgcaatc catcctttcc gaaagagcgg
     2521 gataacgcct ttgcaagaag acgcttgtcc ttttattata agagattatt ttcttctgtc
     2581 catgagtttc ggacggagaa gagaataaaa aaaggtgcgg acaggctccg caccttaggt
     2641 atttcatcat gttatgtgtt agagagaagg tatcaccgcg caaatgattt ttcgagagca
     2701 gaaggtacaa gaagaaccgg gaagcgcgcc ttcagggaag ccgcttcggt agccataacg
     2761 tgttcaacca gttcggcgcg cacggcatcg cgaacctctt cgaacgagag cgcccgagcg
     2821 cgacgccttt caagtacttc gaaaacatga tacccgtatt tcgttt
//
LOCUS       DAOTIL010000291         2859 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminobacteriaceae bacterium isolate AMR_MDS_3026
            NODE_41455_length_2859_cov_3.910128, whole genome shotgun sequence.
ACCESSION   DAOTIL010000291 DAOTIL010000000
VERSION     DAOTIL010000291.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868050
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminobacteriaceae bacterium (wastewater metagenome)
  ORGANISM  Aminobacteriaceae bacterium
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae.
REFERENCE   1  (bases 1 to 2859)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2859)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:19:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,224
            CDSs (total)                      :: 3,198
            Genes (coding)                    :: 3,184
            CDSs (with protein)               :: 3,184
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2859
                     /organism="Aminobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_41455_length_2859_cov_3.910128"
                     /isolate="AMR_MDS_3026"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:3034609"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..459
                     /locus_tag="P5201_06945"
     CDS             <1..459
                     /locus_tag="P5201_06945"
                     /inference="COORDINATES: protein motif:HMM:NF012512.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal-phosphate dependent enzyme"
                     /protein_id="HRV98307.1"
                     /translation="VLGWAHAFEELLASGEIDRLPRIYALQPENCAPLAFAFAKQVDA
                     PEPFSKKETIAEGISSAEPVRGVQVLSAVRRSGGAVRAFSERAIWDGFNKLARKGLYV
                     EPTSAIVGAALDELRNAGELREGERLVALLSGSGLKATDKILSLIGNPPA"
     gene            513..1751
                     /locus_tag="P5201_06950"
     CDS             513..1751
                     /locus_tag="P5201_06950"
                     /inference="COORDINATES: protein motif:HMM:NF020252.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HDOD domain-containing protein"
                     /protein_id="HRV98308.1"
                     /translation="MGRVTVNNLQEGMVLEEDLRAPNGRFILGKGVTLKEKYIRMFKI
                     WGITGADVEGVEDGEVVRRALLEEDAIRRSRSYVDTYFPPEDAEDVCASAVRSFCVRR
                     FAEEFASGTPPSPLPEVKSLPRPPEGHFGRSFSAYALAKDEVRLFSFPDIYFKIQEVI
                     NSPISSATSIAKVVGNDPNLAARLLKLVNSSFYGFPNPIQSIPRAIAIIGSNELTSLA
                     LAVSTMKVFKNVPKEYVDMKSFWRHSIACGVFSRLLAYNKRLRSEERFFLAGLLHDLG
                     RILLYTKAPNEMTYALELSLFEHIPLWRAEKRVFGFDHTAVGKTLMEEWRIPRGISLL
                     CDSHHNPTSEEAPEEAVFVHLADYLANSLRIGTSGSLYFSGVDRALWELLELDEGDVE
                     AVLVQGERQIREIMNIFLMT"
     gene            1759..>2859
                     /locus_tag="P5201_06955"
     CDS             1759..>2859
                     /locus_tag="P5201_06955"
                     /inference="COORDINATES: protein motif:HMM:NF012722.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase dimerization/phospho-acceptor
                     domain-containing protein"
                     /protein_id="HRV98309.1"
                     /translation="MKPSDESIDQLRERVRVLTGEREATKRILEAAVDSLAFPVVVDE
                     TFSSDVLLAQAAQKLRSFIKLDALVFYLFTPDGLDFFPAFCDPPERLSFFEEEMIPLV
                     DDGTFAWAVDRNKPVIITARPGKTSAGLEVKERQLLLHSIMSSNRSIGMFMSLLGEDE
                     TDILDVSFAFFTVLLGSMASILHNAELYTMIQGLNNELQGKIKRLEESERSLAEAMKA
                     KEVFLANVSHEVRTPLNGIVGMTTLLEETPLDARQRELLGVLKDESGALLRLINDLLD
                     FSKMEAGKLVFEDAPFDLYELWNSVRDSFLPRAVAKNLSFSMTLDESAPRVVSGDSFR
                     IRQVLGNLVGNAVKFTGAGSVEVNGNCLARREE"
BASE COUNT          637 a          715 c          785 g          722 t
ORIGIN      
        1 gttcttggat gggcgcatgc attcgaagaa ctgcttgcct caggagagat agaccgcctt
       61 ccgagaattt acgctcttca gcctgaaaac tgcgctcctc tggcgtttgc cttcgcaaag
      121 caggtcgatg ctcccgagcc gttttcaaag aaagagacca tagcggaagg tatcagttct
      181 gcggaaccgg ttcgcggggt tcaggttctt tccgctgtgc gaagaagcgg aggggctgtc
      241 cgtgcctttt ctgagagggc tatttgggat ggctttaata agcttgcacg gaaggggctg
      301 tacgtggagc cgacaagcgc gatcgtcgga gcagcccttg acgagctgcg gaacgcggga
      361 gaactccgcg aaggagagcg tcttgtcgcg ttgctttccg gttccggact gaaagcgacc
      421 gataagattc tctcgctcat cggcaatcct ccggcctgat acttcttctt gctgtggtat
      481 agttatccta ttttgagccg gggagagaaa atatgggccg tgtgactgtg aataaccttc
      541 aggaaggaat ggtcctcgag gaggatcttc gcgccccgaa cgggcgtttc atccttggaa
      601 aaggggtgac cctgaaggaa aaatacatca ggatgttcaa gatttggggg attaccggcg
      661 ccgacgtcga aggcgtcgaa gacggtgaag tcgtccgaag ggcgctcttg gaagaggacg
      721 cgatccggcg gtcccgttcc tatgttgata cgtacttccc tcccgaggac gccgaggatg
      781 tatgcgcttc tgcggtccgt tctttttgcg tgcgccggtt cgccgaagaa tttgcttccg
      841 gaacgccgcc ttctcctttg ccggaagtga aaagccttcc acgccctcca gagggacatt
      901 ttggacgctc tttctctgca tacgcgctgg caaaagacga agtgcgtctt ttctcatttc
      961 cggacattta tttcaagata caggaggtta ttaactctcc gatcagttcg gctacgtcca
     1021 tagcaaaggt ggtcggcaac gatccgaacc ttgccgcccg tctgctcaag cttgtgaaca
     1081 gctctttcta cgggttcccc aacccgatcc agtcgattcc cagagcgatt gcgatcatcg
     1141 gttccaatga actgacctcg cttgcccttg cggtttcgac catgaaggtt ttcaagaacg
     1201 tcccgaaaga gtacgttgac atgaaatctt tttggagaca ttcaatagcc tgcggagtct
     1261 tttcacgcct tttagcatac aacaaacggc ttcgttcgga agagcgcttt tttctcgccg
     1321 gactgctgca cgatctcggc cggatactcc tttacacaaa ggctccaaat gagatgacct
     1381 acgcgctcga actttcgctt tttgaacaca ttcccctctg gcgtgccgag aagcgagtat
     1441 tcggcttcga tcataccgct gtagggaaga cgctcatgga ggagtggaga attccccggg
     1501 ggatcagtct tctctgtgat tcccatcaca accctacgtc ggaagaagcc cccgaagaag
     1561 cggtattcgt ccatcttgcg gattatctgg caaacagtct gcgtattggc accagcggct
     1621 ctttatattt ctcgggagtg gatcgcgcgt tgtgggagct cctggaactg gatgagggcg
     1681 acgtggaggc tgtgcttgtc caaggagagc gtcagatacg cgagataatg aatatttttt
     1741 taatgacgta atgtaaagat gaaaccttca gacgagagta tagaccagct tcgggagaga
     1801 gttcgggtac ttacaggaga aagagaggcg acaaaacgga ttcttgaggc cgccgtcgac
     1861 tctcttgctt tccccgttgt tgtcgatgaa accttctctt ccgacgtctt attggcccag
     1921 gctgcgcaga aactccgctc tttcatcaag ttagacgctc ttgtcttcta tctctttaca
     1981 ccggatgggc tggacttttt ccccgctttt tgcgacccgc ccgaaagact ctcctttttc
     2041 gaggaggaaa tgattcctct cgtggatgac ggaacatttg cctgggctgt cgaccggaac
     2101 aaaccagtca taatcaccgc tcgtcccggg aaaacgagtg cgggtcttga agtgaaagaa
     2161 cgccagcttc tgttgcattc aataatgtcg tcaaaccgca gcatcgggat gtttatgagt
     2221 cttttaggag aggacgaaac agatattctt gatgtctctt tcgccttctt tacagttttg
     2281 cttgggtcta tggctagtat ccttcacaat gccgagctgt ataccatgat tcaaggtttg
     2341 aataatgagt tgcaaggtaa aatcaaacgg ctggaggagt cggaacgtag tcttgcagaa
     2401 gcgatgaagg caaaagaggt ctttcttgcg aatgtcagtc atgaagtgcg tacgccgctc
     2461 aacggtattg tcggtatgac gacactgctg gaggaaacgc ctttggatgc gcgccagaga
     2521 gagctccttg gtgttctgaa ggacgaatca ggagctcttc tccggctgat caacgatctt
     2581 ctggattttt caaagatgga ggcgggaaaa cttgtcttcg aagacgctcc cttcgacctg
     2641 tacgagcttt ggaacagcgt ccgcgacagt ttccttccgc gcgccgtcgc aaaaaacctc
     2701 tcgttttcga tgacgctcga cgaatcggcg ccccgagttg tatccggcga ctccttcagg
     2761 atacgccagg tcctcgggaa tctcgtgggg aatgctgtaa aatttaccgg ggcaggatct
     2821 gtcgaagtca acggtaactg cttggcacgg agggaagaa
//
LOCUS       DAOTIL010000292         2857 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminobacteriaceae bacterium isolate AMR_MDS_3026
            NODE_41499_length_2857_cov_2.644183, whole genome shotgun sequence.
ACCESSION   DAOTIL010000292 DAOTIL010000000
VERSION     DAOTIL010000292.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868050
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminobacteriaceae bacterium (wastewater metagenome)
  ORGANISM  Aminobacteriaceae bacterium
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae.
REFERENCE   1  (bases 1 to 2857)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2857)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:19:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,224
            CDSs (total)                      :: 3,198
            Genes (coding)                    :: 3,184
            CDSs (with protein)               :: 3,184
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2857
                     /organism="Aminobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_41499_length_2857_cov_2.644183"
                     /isolate="AMR_MDS_3026"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:3034609"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(142..411)
                     /locus_tag="P5201_06960"
     CDS             complement(142..411)
                     /locus_tag="P5201_06960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008710650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008219 - cell death [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Txe/YoeB family addiction module toxin"
                     /protein_id="HRV98310.1"
                     /translation="MVGSWRVVFTKAALKDAEKLLSAGLKPKAEQLLALLREDPYRSP
                     PPFEKLVGDLSGACSRRINIRHRLVYQVIETEHTVKVLRMWSHYE"
     gene            complement(405..647)
                     /locus_tag="P5201_06965"
     CDS             complement(405..647)
                     /locus_tag="P5201_06965"
                     /inference="COORDINATES: protein motif:HMM:NF014643.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0110001 - toxin-antitoxin complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0097351 - toxin sequestering activity
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0045927 - positive regulation of growth
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system Phd/YefM family
                     antitoxin"
                     /protein_id="HRV98311.1"
                     /translation="MSDITATEARKRLFQLLDEVADTHVPVRIAGKRNSAVLVGESDW
                     RAMEETLFLCSIPGMRDSIIEGMATPVEECTEDIGW"
     gene            818..1402
                     /locus_tag="P5201_06970"
     CDS             818..1402
                     /locus_tag="P5201_06970"
                     /inference="COORDINATES: protein motif:HMM:NF013618.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase SLT domain-containing protein"
                     /protein_id="HRV98312.1"
                     /translation="MRNGIWGAALCAILSAAIFLAGVFAVKLYAQEIPGTSVGRKAAI
                     GIMSREISAAMTPWKIKGPIRKATEYSRHIWDACELYKVDPFLVAALVCVESEGRADA
                     VSHSRVGLMQVNVQENMAWITKHFPKIKTVDDLLKARNNVMAGAFALSAAMRSSEDVR
                     TALFKYLGTEDERQVGKILDVVGRMNTAAQAGRK"
     gene            1523..2158
                     /locus_tag="P5201_06975"
     CDS             1523..2158
                     /locus_tag="P5201_06975"
                     /inference="COORDINATES: protein motif:HMM:NF015056.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016811 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds, in linear amides
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetamidase/formamidase family protein"
                     /protein_id="HRV98313.1"
                     /translation="MLSVSDVVFSFSPEHPPVARVSPGDMVRFMTKDCFCDQVTGASD
                     LVTAIDFDRVNPATGPVFVEGAEPGDTLCAEILSIDVAEKGIACTLPDSGPLIGWCEL
                     RTKVIEVREGKAIFNDLVFPVRPMVGVIGTAPAEGAVVCGQPGAHGGNLDCKFMTAGA
                     KAYFPVRTRGALFQLGDLHASMGDGEIGGTGIEIGGTVLVRLHLIKNLPLD"
     gene            2180..2422
                     /locus_tag="P5201_06980"
     CDS             2180..2422
                     /locus_tag="P5201_06980"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRV98314.1"
                     /translation="MIHTLASGPEFVAVQREAARQMQRLIAAAWGWDETDAALYLSVR
                     GDSAICQTCQPSTFDMVLRMGIPKEPGKARLLPMPG"
     gene            complement(2492..2692)
                     /locus_tag="P5201_06985"
     CDS             complement(2492..2692)
                     /locus_tag="P5201_06985"
                     /inference="COORDINATES: protein motif:HMM:NF018249.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRV98315.1"
                     /translation="MAKADPERVIILRSVSNFAMQWKGASAYESLMSEGGDEYSSVEA
                     AVRNVHAVGAAVLRELLKERTP"
BASE COUNT          552 a          821 c          891 g          593 t
ORIGIN      
        1 agagtgtagg actgcccaag tgttgcggaa atcttgcagt attgtaacac aaagagaaga
       61 gcgaaaagcg aggagcacaa aggattaggg gttccaatct gcggactccc gaggagcccg
      121 gtaccctccc gtagttgtac gctattcgta atggctccac atgcggagca cttttaccgt
      181 atgctccgtt tcgatcactt ggtagaccag acgatggcgg atgttgattc ggcgggaaca
      241 tgcgcctgaa agatcgccga cgagcttttc aaaaggcgga ggagatcggt aggggtcttc
      301 acgcagtagc gcgaggagct gttccgcctt gggtttcagc cccgccgaaa gcagcttttc
      361 tgcatctttc agagccgcct tggtgaaaac gactctccac gatcctacca tccgatatcc
      421 tcggtgcact cttccaccgg tgttgccatt ccttcgatga tggagtcgcg cattccgggg
      481 atgctgcaca gaaagagcgt ctcttccatg gccctccagt cggactcgcc gaccagcacg
      541 gccgagttgc gttttcccgc gatgcgcacc ggaacgtggg tatccgccac ctcgtcaaga
      601 agctgaaaga gccgttttcg ggcttccgtc gctgtgatgt cgctcatatc ctcacctcct
      661 gtggtacgtt ctatggtaca tgatgtctgc ggcgaggtca aacgccggag tgcagcgtca
      721 ccaccgttcg ctcttcccaa gaagccgtat ttgtcacgat gccttcctct ttcttcgatg
      781 ctatcatgct cgaaaacatg cgaaggagcg tgtgctcatg cgaaacggaa tatggggcgc
      841 tgctttgtgc gcgatcctgt cggcggcgat ttttctcgcg ggagtgtttg ctgtaaaact
      901 ctatgctcaa gaaatcccgg gaacgtcggt cggaagaaaa gccgccatcg gcatcatgtc
      961 gcgcgagatc agcgcggcga tgaccccgtg gaagatcaag gggccaatac ggaaggcgac
     1021 ggagtactcg cggcatattt gggacgcctg cgaactctac aaggtcgacc cctttctggt
     1081 cgccgcgctg gtctgcgtgg agtcggaggg gcgggcggac gccgtttcgc attcacgggt
     1141 cggcctgatg caggtgaacg tgcaggagaa tatggcctgg atcacgaagc acttcccgaa
     1201 gatcaagacc gtggacgacc tgctcaaggc gcggaacaac gtgatggcgg gggcgttcgc
     1261 cctctccgcc gcgatgcgga gttcggagga cgtccggacg gcgctgttca aatacctcgg
     1321 tacggaggat gaacggcagg ttgggaagat actcgacgtc gtcggtcgaa tgaacacggc
     1381 ggcgcaagcc ggaagaaaat aagagaaaga atacggaaat cgcggtgtgt tgaagtacgg
     1441 ggggaccccg tccgcgtgtt cctttccttg cgcgcttctc cctgctacaa tgtgaagaaa
     1501 cacaccagaa ggaggttccg ccatgctttc agtttccgac gtggtctttt ccttttcgcc
     1561 cgaacatccg cccgtagcgc gggtgagtcc cggggatatg gtgcgcttca tgacgaagga
     1621 ctgtttctgc gatcaggtga cgggagcgtc cgacctcgtg acggcgattg atttcgacag
     1681 ggtgaacccc gccacaggcc cggtcttcgt ggagggcgcc gagccggggg atacgctgtg
     1741 cgcggagatt ctttccatcg acgtcgcgga aaagggaatc gcctgcacgc tgccggattc
     1801 cgggccgctg atcgggtggt gcgagctgcg caccaaggtg atcgaggtac gcgaggggaa
     1861 agcgatcttc aacgacctcg tcttcccggt gcgtccgatg gtcggcgtca tcggaacggc
     1921 tcccgccgag ggcgcggtgg tctgcggcca gcccggagcg cacggaggca acctcgactg
     1981 caagttcatg accgcgggcg ccaaggcgta tttccccgtt cggacacggg gcgcgctctt
     2041 tcagctcggc gatctgcacg cttcgatggg cgacggcgag atcggcggta cggggatcga
     2101 aatcggggga acggtgcttg tccggctgca cttgatcaaa aatctgccgc tcgactgacc
     2161 tatgttggag acaggcgaca tgatccatac gctcgccagc ggtccggagt tcgtcgctgt
     2221 acagcgggag gccgcacgcc agatgcagcg gctgatcgcg gcggcctggg gatgggacga
     2281 aacggacgcg gcgctttacc tttccgtgag gggcgattcc gcgatctgcc agacctgcca
     2341 gcccagcaca ttcgacatgg tgctgcgcat gggaataccg aaagaaccgg ggaaggcgcg
     2401 ccttctcccc atgccgggat aaagtgaaaa gagaggcttc ccgtccggga ggcctctctc
     2461 gaatttccgt atgcctgcat gcgccgttcc gtcagggcgt ccgctccttc agcagctcgc
     2521 gcaacacagc ggcgccgacg gcgtggacgt tgcggactgc cgcttcgacc gagctgtatt
     2581 cgtcgccccc ctcgctcatc aggctctcgt aggccgaagc ccccttccac tgcatggcga
     2641 agttggaaac cgacctgagg atgatcaccc gctccggatc ggcctttgcc agttgagaga
     2701 ggctggcgcc ttcgtcttcc cgcgtgctca ggtcgacgat agcctctgcg cttacctcct
     2761 tatttcttca agatagagat gaatacgttg ctgcaggctt tctctgtcga tcattccggt
     2821 gcttgtctgt accgtgtaca tcttctccgg cacgacg
//
LOCUS       DAOTIL010000293         2853 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminobacteriaceae bacterium isolate AMR_MDS_3026
            NODE_41554_length_2853_cov_4.494639, whole genome shotgun sequence.
ACCESSION   DAOTIL010000293 DAOTIL010000000
VERSION     DAOTIL010000293.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868050
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminobacteriaceae bacterium (wastewater metagenome)
  ORGANISM  Aminobacteriaceae bacterium
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae.
REFERENCE   1  (bases 1 to 2853)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2853)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:19:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,224
            CDSs (total)                      :: 3,198
            Genes (coding)                    :: 3,184
            CDSs (with protein)               :: 3,184
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2853
                     /organism="Aminobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_41554_length_2853_cov_4.494639"
                     /isolate="AMR_MDS_3026"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:3034609"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            441..857
                     /locus_tag="P5201_06990"
     CDS             441..857
                     /locus_tag="P5201_06990"
                     /inference="COORDINATES: protein motif:HMM:NF013970.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIN domain-containing protein"
                     /protein_id="HRV98316.1"
                     /translation="MSRTKIFIDTNILIYTVDAHDAKKQIQARSIVKSVVENDLPVIS
                     TQILQEFYSASTTKLKVDPLIAKNLVHNFGNMEVVPVDPHTIEQGIDISILCRISFWD
                     GLVVAAAEQANCEILFSEDLNNGQTIRGIKILNPFF"
     gene            1045..2046
                     /locus_tag="P5201_06995"
     CDS             1045..2046
                     /locus_tag="P5201_06995"
                     /inference="COORDINATES: protein motif:HMM:NF015056.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016811 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds, in linear amides
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetamidase/formamidase family protein"
                     /protein_id="HRV98317.1"
                     /translation="MFLMLLFVLTLLLLLAASPSEAKTHSFFITKEQAYNGMLRSDIP
                     PALTIEDGDTVVFNTVMLMAGELSPEMTFDEMFALRTRMKERGLGVYAFTGPFFVKGA
                     EPGDVLEVRIKRIVPGKYAVTHIYPDETGIGGLPEGFETGYLKPLYLSEDRKSIPFAP
                     GVTVPVRPFLGTMAVAPRPGEEHPPAVPGYFAGNMDNKELGAGSTLYIPVNVPGALFM
                     AADAHAVQGDGEVSIAAAETYFEEVELEFVVRKDLPLRFPMAETPDHWIVMAFHKDLD
                     EAMKGAIREAIRFLEREKRLTRNEAYSLCSLAVDFRVTQVVDGDKGVHGMIPKAIFG"
     gene            complement(2146..>2853)
                     /locus_tag="P5201_07000"
     CDS             complement(2146..>2853)
                     /locus_tag="P5201_07000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013048571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015205 - nucleobase transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NCS2 family permease"
                     /protein_id="HRV98318.1"
                     /translation="WGILIITGIAVFLGVAKAPEALVSTPPSVMPIFMKMDFSGLMNP
                     NFWIVMFSFFFVDFFDTVGTLVGVTNRAGMLDAEGRLPRAREALMADAIGTTAGAVLG
                     TSTVTSYVESASGVEQGGRTGLTALVTGVLFLLALFFSPIVSIVPACATAPALVLVGV
                     FMMASLKDLDFSDWTDFVPASVAIFVMPFSYSIAHGIEFGIISFVVLKVLGGKAKDVS
                     PIMLVLAALFVAKEIFF"
BASE COUNT          629 a          828 c          841 g          555 t
ORIGIN      
        1 gcctcggaga acggcgtcct ctggcaggcc gaacacccct ccgagtcgta ccagaccctc
       61 cggcttcgcc cgggctcgaa ggtgcgtttc gccgtccctc cggcaggcat cgagtcgtgg
      121 agcggggggc cttctcctcg ataatgcaac tgcatactct tccattcgat atcttgacag
      181 cacgtaagtg cgcacgtatt atgtgtgaat aggaggtgga atcatgcgta atataacgct
      241 ttctctcgat aaagagacgc tccgacgggg aagagagtac gcgaaacgcc ataatatttc
      301 ttttaacgcg ctcgtacgtc gtttggtcga gcaaacggtg gcgtcctctt cttcccagtg
      361 gctggaagac acattcgctc tcatggatca aacgcccgtc tcgtcgcaag gtgagaaatg
      421 gtcgagggaa gagttgcatc gtgtctagaa ccaaaatatt catcgacacc aacattctga
      481 tatatacagt tgacgctcac gacgccaaaa aacagattca ggcccgttcc atagtgaaaa
      541 gcgttgtgga gaatgatctt cctgtgattt ccacacagat tttgcaggag ttttacagcg
      601 cctctacaac caagttgaag gttgatcctc ttattgcaaa aaacctcgtt cataacttcg
      661 gcaacatgga ggttgttcct gttgatccgc acacgatcga acaaggtatc gacatcagta
      721 ttctctgtcg gatttccttc tgggacggac ttgtcgttgc agcggcagag caggccaatt
      781 gcgagattct cttttcagaa gacctgaata atggccaaac aatacgggga ataaagatcc
      841 tcaacccgtt tttttaacct ttttcacagc ccgaaagccc gcttgcatca acgtcgcgta
      901 ccgaacggaa gccgccgtac gaacttctcc gctctccgtt cgggagaatt ttcgcgccct
      961 ctcacccgag aaaagaagct gacggcgcgt tcactccccg gatgatatac tttcttcaga
     1021 ctttcacatc gaggaggaac acgcatgttt ttaatgctgc tttttgtact gacgcttctg
     1081 ctgctcctgg ccgcttctcc ctccgaggcg aagacgcact ccttcttcat cacgaaggaa
     1141 caggcgtaca acggcatgct ccggagcgat atcccccccg cgctcacgat agaggacggc
     1201 gacacggtcg ttttcaacac ggtgatgctg atggccggag agctgtcgcc cgagatgacg
     1261 ttcgatgaga tgttcgccct tcgcacgcgc atgaaagagc gcggcctggg cgtgtacgcc
     1321 ttcaccgggc cgttcttcgt caagggggcc gagccgggag acgtcctcga agtccgtatc
     1381 aagcgcatcg tccccggcaa gtacgccgtc acgcacatct accctgacga aacggggatc
     1441 ggcggccttc cagaggggtt cgagaccggg tatctcaaac cgctgtacct ctcggaggac
     1501 cggaaaagca tccccttcgc tcccggcgtg acggttcccg tccgtccctt cctcgggacg
     1561 atggcggtgg ccccgcgtcc cggcgaggag caccctcccg cagtcccggg ctacttcgcg
     1621 gggaacatgg acaacaagga gctgggggcg ggaagcacgc tctatatccc ggtgaacgtg
     1681 ccgggggcgc tcttcatggc cgccgacgcg cacgccgtgc agggggacgg cgaggtgagc
     1741 atcgccgcgg cggagaccta cttcgaggag gtcgagctgg aattcgtcgt ccgcaaggac
     1801 ctgccgctgc gctttccgat ggcggagacg ccggaccact ggatcgtgat ggccttccat
     1861 aaagacctcg acgaagcgat gaagggggcc attcgcgagg cgatccgctt cctcgaacgg
     1921 gagaagcggc tcacccggaa tgaagcctac tccctgtgca gtttggcggt cgacttccgg
     1981 gttacccagg tagtagacgg agataaggga gtgcacggca tgatcccgaa ggcaattttc
     2041 gggtaagcga caccggcacg gacagcccat gaaaaagggg agcgccctgc ggttcggagc
     2101 gctccccttc gcgtagtgta ctgtgcctga tcggccggcg cggcgctaga agaatatctc
     2161 cttggcgaca aagagcgctg ccaaaacaag catgatcggg ctgacatcct tcgccttgcc
     2221 tccgagtacc ttgaggacca cgaaggagat gatgccgaac tcgataccgt gagcgatgct
     2281 gtagctgaag ggcatgacga agatcgccac gctcgccggc acgaagtcgg tccagtcgct
     2341 gaagtccagg tccttcaagc tcgccatcat gaagacgccg acgagaacga gggcgggagc
     2401 tgtggcgcac gccggaacga tggagacgat gggagagaag aagagagcca gcagaaagag
     2461 aacgccggtc acaagagcgg tcagtccggt gcgtccgccc tgctccacgc cgctggcgct
     2521 ctcgacgtag gaggtgacgg tggacgttcc gaggacagcg cccgccgtgg tgccgatggc
     2581 gtcggccatg agggcctcgc gggccctggg aagccgtccc tcggcgtcaa gcattccggc
     2641 gcggttggtc acgccgacaa gcgtcccgac cgtatcgaag aagtcgacga agaagaagga
     2701 gaacatgacg atccagaagt tcggattcat cagtccggag aagtccatct tcatgaagat
     2761 cggcatgacg gagggaggcg tggacaccag cgcttcgggc gccttggcca cgcccaggaa
     2821 gaccgcaatc cccgtgatga taaggatacc cca
//
LOCUS       DAOTIL010000294         2843 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminobacteriaceae bacterium isolate AMR_MDS_3026
            NODE_41727_length_2843_cov_3.318149, whole genome shotgun sequence.
ACCESSION   DAOTIL010000294 DAOTIL010000000
VERSION     DAOTIL010000294.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868050
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminobacteriaceae bacterium (wastewater metagenome)
  ORGANISM  Aminobacteriaceae bacterium
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae.
REFERENCE   1  (bases 1 to 2843)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2843)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:19:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,224
            CDSs (total)                      :: 3,198
            Genes (coding)                    :: 3,184
            CDSs (with protein)               :: 3,184
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2843
                     /organism="Aminobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_41727_length_2843_cov_3.318149"
                     /isolate="AMR_MDS_3026"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:3034609"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..482
                     /locus_tag="P5201_07005"
     CDS             <1..482
                     /locus_tag="P5201_07005"
                     /EC_number="4.3.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012868827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008851 - ethanolamine ammonia-lyase
                     activity [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ethanolamine ammonia-lyase light chain EutC"
                     /protein_id="HRV98319.1"
                     /translation="RPDLGRQFSAEAKEAMKRIAGPSPKVLVYVSDGLSSTAVETNAM
                     DTLKMIGQGLERQGVKLAKPFFVKYGRVPAMDVVSEVTGADVVCVLLGERPGLVTAES
                     MSAYIAYKATVGMPEAKRTVVSNIHAGGTPAVEAGSYIADIIKLMLEKKISGIDLKL"
     gene            513..1166
                     /gene="eutL"
                     /locus_tag="P5201_07010"
     CDS             513..1166
                     /gene="eutL"
                     /locus_tag="P5201_07010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012868828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031469 - bacterial microcompartment
                     [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization microcompartment
                     protein EutL"
                     /protein_id="HRV98320.1"
                     /translation="MKGDPLKATLLSVKIIPNVNPEMAASLNLSPEQRSLGIITADSD
                     DVTYTALDEATKKADVAVVYAKSFYAGAANANTKLAGEVIGILAGPNPAEVRSGLNAV
                     ADFIENDAWFVSANEENTIPYYAHCVSRTGSYLSKVAGVNEGDAIAYLIAPPLEAMYA
                     LDAALKAADVKLAAFYGPPSETNFGGGLLTGSQSACKAACDAFAQAVIFVAENPVLY"
     gene            1174..1410
                     /locus_tag="P5201_07015"
                     /pseudo
     CDS             1174..1410
                     /locus_tag="P5201_07015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009201841.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="BMC domain-containing protein"
     gene            1800..>2843
                     /locus_tag="P5201_07020"
     CDS             1800..>2843
                     /locus_tag="P5201_07020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012868830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HRV98321.1"
                     /translation="MTQLQDRDLLSVQEVRNLVAKAKEAQKRLASMSQGKIDELVAAV
                     AEAACRHAERLGRMAHEETGFGKPEDKTLKNLFAAKSVYESIKDLRTVGIVREDTKNR
                     MFEVAVPVGVIAGLIPSTNPTSTAIYKAMVALKAGNGIVFSPHPSAKGCILETIRVLS
                     EAVCAAGGPEGIFGCMTIVTLEGTEELMHSKDVALILATGGSGMVRAAYSSGTPAIGV
                     GPGNTPAFIERSADIPQAVRRIISSKTFDYSTICASEQSVVAEACIESVVRAEFEKQG
                     AYFLPPEDAAKVARAIFTPSGGMNPRTVGKSPKFIAEKAGVAIPEGTKLLLADGMGVG
                     KEYPFSHEKLCPAL"
BASE COUNT          547 a          813 c         1027 g          456 t
ORIGIN      
        1 cgcgcccaga cctcggacgg cagttcagcg cggaggcgaa agaggcgatg aagcgcatcg
       61 ccggaccgtc tcccaaggtg ctggtgtacg tttccgacgg gctcagctcc accgcggtgg
      121 agaccaacgc gatggacacg ctgaagatga tcgggcaggg tttggagcgg cagggcgtga
      181 agctcgcgaa gccgttcttc gtgaagtacg ggcgcgttcc ggcgatggac gtcgtctccg
      241 aagtgacggg ggcggacgtc gtctgcgtgc tgctcggcga acgtcccgga ctggtgaccg
      301 ccgagagtat gtcggcctat atcgcctaca aggcgacggt cggtatgccg gaggcgaagc
      361 gcaccgtggt gtcgaacatc catgccgggg gaactccggc ggtggaggcg ggcagttaca
      421 tcgcggacat catcaagctg atgctcgaga agaagatcag cggcatcgat ctgaaactgt
      481 aagtatttct tactgtcggg agggaagatg aaatgaaggg cgatcccttg aaggcaacgc
      541 ttttgagcgt gaagatcatc cccaatgtga atccggagat ggcggcttcg ctgaacctga
      601 gtccggagca gaggagcctc ggcatcatca ccgcggacag cgacgacgtg acctacacgg
      661 cgctggacga ggcgacgaag aaggcggatg tggcggtggt gtacgcgaag tcgttctatg
      721 caggcgccgc gaatgcgaac acgaagctcg cgggagaagt tatcggcatc ctcgcggggc
      781 cgaaccccgc cgaggtgcgg agcggactga acgccgtcgc ggacttcatc gaaaacgacg
      841 cgtggtttgt ctcggcgaac gaggagaata cgattccgta ctatgcgcac tgcgtctccc
      901 gcacgggaag ctatctttcc aaggtggcgg gagtgaacga gggcgacgcg atcgcctacc
      961 tgatcgcgcc tccgcttgag gcgatgtacg cgctcgacgc ggcgctgaag gccgcggacg
     1021 tgaagctggc ggcgttctac ggacctccct cggagaccaa cttcggcggc ggcctgctca
     1081 ctggctcgca gtcggcgtgc aaagcggcct gcgacgcttt tgcccaggca gtgatcttcg
     1141 tcgcggagaa cccggttctc tactaacggt ttcatggaga gcagagccct gggaatgatc
     1201 gaagtccgcg gaatgctcgg cgcggtcgag gccgcggacg cggcgctgaa atcggccgcc
     1261 gtgcgtctgc tgcggtcgag gaaagtcggc ggcgggctgg tgacggtctt cctgacggga
     1321 gaggtcgccg ccgtccggtc ggcggtggac gccgccgtcg cgtcattgaa caggctcgga
     1381 ctgcagtgtt cgtcgcacgt gatcccgcgt cttacgccgg atgttttcac gatgatcgcg
     1441 gagggtgggg gcgcgcattc gaaacaggcc gcgcccgaag cgccgcgcgg gccgtcttct
     1501 accggagatg ccgtttctgc ggagaaagat gtttccgggg agggcccgtc cggctgcgtg
     1561 atgaaggatg cggccggcga agaggacgat gtcgaggaag ccgaggacga gggcgatgtc
     1621 ggcgcggtat ggagcgggga cgcccccgac ctcgagaaca tgacggtgat ggagcttcgt
     1681 cgtcttgccc gcaccgtcgg gctggagacg atgacacgaa agcagatacg cttcgccgga
     1741 aaggcggagc tgcagcgcga actgacggag ttttggaaga accggggagg tgagagcgag
     1801 tgacgcagct tcaggacaga gatctgctct ccgtacagga ggtccgcaac ctcgtcgcga
     1861 aagcgaagga ggcgcagaag cgcctcgcct ccatgagtca gggcaagatc gacgaactcg
     1921 tggcggcggt tgccgaagcg gcgtgccggc acgcggagcg gctggggagg atggcgcacg
     1981 aggagaccgg tttcggcaag cccgaggaca agaccctcaa gaacctcttc gccgcgaaga
     2041 gcgtgtacga atcgatcaag gacctccgga cggtcggcat cgtccgggag gatacgaaga
     2101 acaggatgtt cgaggtcgcc gtccccgtcg gcgtcatcgc ggggctgatt ccctcgacga
     2161 acccgacctc gacggcgatt tacaaggcga tggtcgcgct gaaggcgggg aacggcatcg
     2221 tcttctcccc gcacccgagc gcgaaggggt gcatcctgga gacgatacgc gtgctctccg
     2281 aggcggtctg cgcggctgga ggcccggaag ggatcttcgg ctgcatgacg atcgtcaccc
     2341 tcgaaggaac ggaagagctg atgcactcga aggacgtggc gctgatcctg gccaccggcg
     2401 ggtcggggat ggtccgggcg gcgtacagct ccggaactcc ggccatcggc gtcggcccgg
     2461 ggaacacacc ggccttcatc gagcggtcgg cggacattcc gcaggccgtc cgcagaatca
     2521 tttcgagcaa gacgttcgac tacagcacca tctgcgcctc ggagcagtcc gtggtggcgg
     2581 aggcgtgcat cgaatcggtc gttcgggccg aattcgagaa gcagggcgca tacttcctgc
     2641 cgcccgaaga tgcggcgaag gtggcccgcg ccatctttac gccgtctggg ggaatgaacc
     2701 cccgcacggt ggggaagagc ccgaagttca tcgccgagaa ggcaggggtc gccatcccgg
     2761 aggggacgaa gctgctgctc gcggacggca tgggcgtggg gaaggagtac cccttctcgc
     2821 acgagaagct ctgcccggcg ctc
//
LOCUS       DAOTIL010000295         2842 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminobacteriaceae bacterium isolate AMR_MDS_3026
            NODE_41748_length_2842_cov_4.398278, whole genome shotgun sequence.
ACCESSION   DAOTIL010000295 DAOTIL010000000
VERSION     DAOTIL010000295.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868050
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminobacteriaceae bacterium (wastewater metagenome)
  ORGANISM  Aminobacteriaceae bacterium
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae.
REFERENCE   1  (bases 1 to 2842)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2842)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:19:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,224
            CDSs (total)                      :: 3,198
            Genes (coding)                    :: 3,184
            CDSs (with protein)               :: 3,184
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2842
                     /organism="Aminobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_41748_length_2842_cov_4.398278"
                     /isolate="AMR_MDS_3026"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:3034609"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            200..700
                     /gene="rimP"
                     /locus_tag="P5201_07025"
     CDS             200..700
                     /gene="rimP"
                     /locus_tag="P5201_07025"
                     /inference="COORDINATES: protein motif:HMM:NF014617.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000028 - ribosomal small subunit assembly
                     [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0042274 - ribosomal small subunit
                     biogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimP"
                     /protein_id="HRV98322.1"
                     /translation="METGRKDGWKSLRAIVEGLGYECVGIALVTEERAVFLRVFIDSV
                     GGIQVRDCEIVSKAVSAFLDEHEEYIPGKYFLEVSSPGIERPLFTPEDYKRFIGKKAR
                     LRVRLPVQGRKSLSGLILSADDDAVELEIDADTPEEMTAVRISFDIITKGNLAFEEKK
                     DKRRNS"
     gene            702..1805
                     /gene="nusA"
                     /locus_tag="P5201_07030"
     CDS             702..1805
                     /gene="nusA"
                     /locus_tag="P5201_07030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008709796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor NusA"
                     /protein_id="HRV98323.1"
                     /translation="MQLGRDFARALAQITKERGLSQEVIASSLEAALISAYKKFQGGN
                     QDVEVFIDTENGDISICEVKQIVDVVTSPDVQISLEDAQSQGFVDVAEGDYIRIERDP
                     ENFGRIAAQTARQVIIQRLKDAERQIIFEEFSDRVGDLVTGVVFKSENDQVLIRLNDR
                     TEAILPREERIVSEKYIPGERMKLYLLDVRQTTRGPRIVVSRTHPGLLRKLLELEVPE
                     IREGVIDIKNIVREAGARAKIAVQTLDVNVDPVGACVGNNGARIKSISKELCGEKVDV
                     IVWSSDPLQYVRNALSPAKIVKIEPILEQEKAVTVYARPDQLSLAIGKAGQNVRLAAR
                     LTGWKIDINALEPERMPTLHDIFQDIIEEEGSK"
     gene            1817..2101
                     /locus_tag="P5201_07035"
     CDS             1817..2101
                     /locus_tag="P5201_07035"
                     /inference="COORDINATES: protein motif:HMM:NF016204.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlxR family protein"
                     /protein_id="HRV98324.1"
                     /translation="MKRRRPRTCVACREESPKREMLRVVRTPEGSVRLDLSGKAPGRG
                     AYVCVSEECLAAARKRDALAKALKTKVEDAVYEEILAVLREKKKERAETP"
     gene            2098..2436
                     /locus_tag="P5201_07040"
     CDS             2098..2436
                     /locus_tag="P5201_07040"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRV98325.1"
                     /translation="MNEQALATLFSLLGFARRSGSLLIGQDIVKSSLVRRESLLVLFA
                     EKDSSFYRSLQKGRYAEKCLLLNPPGISEADLSAAIGSRNVKVVALPLRGGFAQRILQ
                     LLAEGGERIE"
     gene            2429..>2842
                     /locus_tag="P5201_07045"
     CDS             2429..>2842
                     /locus_tag="P5201_07045"
                     /inference="COORDINATES: protein motif:HMM:NF016635.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-2 N-terminal
                     domain-containing protein"
                     /protein_id="HRV98326.1"
                     /translation="MSKIRVYELAKMLGKSNKELLDLLIDLGADVKSHMSSIDNEIAQ
                     LVEDALSSTSSSGTQKTKKEEQKVTKTETFEVSKGSTVKAVAELLGVTPAEAVKSLIS
                     AGMMVPADSPVDERCLEVLGEAYDAVLTLACDDAEQ"
BASE COUNT          695 a          620 c          862 g          665 t
ORIGIN      
        1 ccccatggag ttcgagacgt atcttgggga gtggtgggag gggtttgttt ccgattttta
       61 tctgtaaaat tgtggagtga cgctgcgcaa agactttttt tgtgctatac tctataatga
      121 tttttttgcg ggattttttg caaagagtgg gatttccccc actctttgtt ttttacgaag
      181 agtgaatcgg ggcgataata tggaaacagg cagaaaagat ggatggaaat cgttgcgtgc
      241 aatcgtggaa ggcctcggat acgaatgtgt cgggattgct ctcgtcaccg aagaaagggc
      301 tgttttcctg cgtgttttta ttgattccgt gggaggcatt caggtccggg attgcgaaat
      361 tgtctctaag gccgtgagcg cgttccttga tgaacatgaa gaatacattc cgggcaagta
      421 tttcctggaa gtgagttctc cggggattga gcgtcctttg tttacgccgg aggactataa
      481 acgttttatc ggaaagaagg cgcgactgag ggtgcgccta cccgttcaag gaaggaagag
      541 tctcagcgga ctcatcctct ccgccgatga tgatgcggtc gagctcgaaa tcgatgcgga
      601 tactcccgag gagatgacag ctgtccgtat ttcatttgat attataacta aagggaatct
      661 tgccttcgag gagaagaaag ataaaaggag gaattcttaa aatgcagctt ggtcgtgact
      721 tcgcccgtgc tctggctcag atcactaagg aacgaggtct ctcgcaggaa gttattgcgt
      781 cgagccttga ggcggcgttg atttcggcgt ataagaaatt ccagggaggg aatcaggacg
      841 tcgaagtttt tatcgatacg gagaacggcg atatctccat ctgcgaggtg aagcagattg
      901 tggatgtcgt gacgagtccg gatgtccaga tatccctgga ggatgcacag tctcaggggt
      961 ttgtcgatgt agccgaggga gattacatca ggatagagcg cgatccggag aacttcggcc
     1021 gaattgccgc ccagacagcc cgtcaggtga ttatccagag gctcaaggat gcggaacggc
     1081 agataatttt cgaggagttt tcggataggg tgggggatct cgtgaccgga gtggttttca
     1141 aatccgagaa cgaccaggtg ctcatccgtc tgaacgatag aaccgaggcg attcttccac
     1201 gtgaggagag aatcgtgagt gaaaaatata tcccggggga gcgtatgaag ctctatctgc
     1261 tcgatgtcag gcagacgacg cgaggtcctc gtatcgtcgt ttcaaggacg catccgggtc
     1321 ttctcagaaa acttcttgag ctggaggttc cggagattcg cgagggagtc atcgatataa
     1381 aaaatattgt ccgggaggcc ggagcccggg caaagatcgc cgtccagacg cttgatgtca
     1441 atgtggatcc cgttggcgca tgcgtcggaa ataacggggc gaggatcaag tcgatcagca
     1501 aggagctctg cggcgagaaa gtggacgtca tcgtctggag cagcgacccg ctgcagtacg
     1561 tacggaatgc gctttctccg gcgaaaatcg tcaagatcga acctatcctt gagcaggaaa
     1621 aggcagttac agtgtatgcg cgtccggatc agctttccct cgctatcggg aaggccgggc
     1681 agaatgtgcg tcttgcggcc cgcttgaccg gctggaaaat tgatatcaat gcgcttgagc
     1741 cggagagaat gcccacgctg cacgatattt tccaggatat tatcgaagag gaggggtcca
     1801 aataagcgac gccgtcttga agcggcggcg cccgagaacc tgcgtcgcct gccgcgagga
     1861 gtctccgaaa cgagagatgc tgcgcgtggt gaggactcct gaaggctctg tcaggcttga
     1921 tctttccggg aaggcgccgg ggcgcggcgc gtatgtctgc gtgtctgaag agtgtcttgc
     1981 cgctgcgaga aaaagagacg ctcttgcaaa ggcgttgaaa acgaaagtag aagacgcggt
     2041 ttacgaggaa atccttgccg ttctgcgcga aaaaaagaag gagcgtgcgg agacgccgtg
     2101 aacgaacagg cgctcgcgac tctgttttcc cttcttggtt ttgcgcgtcg ttcgggatct
     2161 ctgctaatcg ggcaggatat agtaaaatct tctttggtgc ggcgggagtc tcttcttgtg
     2221 ctctttgccg aaaaagactc ttcgttttac agatccctcc aaaaagggag gtatgcagaa
     2281 aaatgtctct tgttgaatcc gcctggaatt tcagaggctg atctgtctgc ggcaataggt
     2341 tcgagaaacg tgaaagtggt tgctttaccg ttacgcggcg gttttgcgca gagaatactg
     2401 caactgcttg cggaaggggg agaaagaatt gagtaaaatc cgtgtatatg aacttgcgaa
     2461 gatgctgggg aagagcaata aagagctgct cgatcttctg atagaccttg gcgccgatgt
     2521 aaaaagccac atgagttcca ttgacaacga aatcgcccag cttgtggagg atgcgctctc
     2581 aagcacatcg tccagcggga cccagaaaac aaagaaagag gagcaaaaag tgacgaaaac
     2641 cgaaactttc gaagtgtcaa agggctctac agtaaaagct gtggccgagc tcttgggagt
     2701 gactcctgcc gaagcagtca aaagtcttat ctccgcgggg atgatggttc ccgcggacag
     2761 tccggttgat gaacgatgcc tggaagtcct cggcgaggcc tacgacgccg tgctcactct
     2821 tgcctgtgac gacgcggaac ag
//
LOCUS       DAOTIL010000296         2834 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminobacteriaceae bacterium isolate AMR_MDS_3026
            NODE_41922_length_2834_cov_3.305146, whole genome shotgun sequence.
ACCESSION   DAOTIL010000296 DAOTIL010000000
VERSION     DAOTIL010000296.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868050
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminobacteriaceae bacterium (wastewater metagenome)
  ORGANISM  Aminobacteriaceae bacterium
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae.
REFERENCE   1  (bases 1 to 2834)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2834)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:19:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,224
            CDSs (total)                      :: 3,198
            Genes (coding)                    :: 3,184
            CDSs (with protein)               :: 3,184
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2834
                     /organism="Aminobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_41922_length_2834_cov_3.305146"
                     /isolate="AMR_MDS_3026"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:3034609"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..907)
                     /locus_tag="P5201_07050"
     CDS             complement(<1..907)
                     /locus_tag="P5201_07050"
                     /inference="COORDINATES: protein motif:HMM:NF015894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FapA family protein"
                     /protein_id="HRV98327.1"
                     /translation="MDELRIANLERLVTEIDHFLSGTKETGALPAAESRDASWELRVS
                     DDRLSAWLETYPQVGAGIPLDAAEIIEALEEAGLCLLDPGKIRTIIEKCNKGEEVRGT
                     EALVASGTATVDPVEGKIEFLVPFEKVKLVDEADERAIDWKNLWSIPIVHTGDIIARV
                     HPPQEGVEGADIYGQPLPPRRPAPFRVKFGEGVVVSEEDGMTEVLTARDVGQPVFRGG
                     ELDVLPVLEVPGDVDILSGDIDFIGSVVVRGSVTEGFSVKAGRDVAVSGSAFNARIEA
                     SGSCIVEGGIVGEHCEIRAGEDIRAG"
     gene            complement(940..2565)
                     /locus_tag="P5201_07055"
     CDS             complement(940..2565)
                     /locus_tag="P5201_07055"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HRV98328.1"
                     /translation="MEKWNEELLKSIESIASEAGVRPDGKDSGTMEGLKPEELHSNDI
                     VFRSTSRAVVVCRMLFNARGIPVDFLLESVNPAFCRIFGIAPESAAGRSGKEFFMPEG
                     KEIPALDLFDRALKEQQAFSFMFSSEKLSKVFDVLAFSLENGTFCMAITDATSQVAAE
                     RERRENERFTEKVFGSVDVWIVLLDERGRVVFWNAAAERLSGISADEALSGSFDFRSV
                     FCPGEEDREDAIGKMDACRRDGATEVRLETCVCAAEGRSLRVEWTVRPWHFEDVSSDG
                     LLLLGRDVTQDRHTEEQLLESERRYAAAFKETMAPMLLVDPETGAVCDCNAAALALYG
                     YSREEMLALTNRDFNLLPAEDLRKNMEEATARLRNRFVFQHRLKNGDIRTVEVFASPT
                     PFQGKTFLFSILHDITEKQRLEEQLHLVTFDCALQAKILGGILESSPDYLYVFDRMRK
                     FCFSGKKGAALFERTPEEMTGRSWEALGIPEERIAAFDEHLAQTFAAGKSKRGVLVFP
                     APHGDMPLEYALYPICSENGVVDMVFCSMHAVP"
     gene            complement(2597..>2834)
                     /locus_tag="P5201_07060"
     CDS             complement(2597..>2834)
                     /locus_tag="P5201_07060"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRV98329.1"
                     /translation="QYHGKMKSSVAELAARIDDEKRASIAEKRIMPRIRIRDRVYPNV
                     TLKIWGQTLVVKQQEFHVSFFMDKDRGVIARGGF"
BASE COUNT          577 a          921 c          752 g          584 t
ORIGIN      
        1 accccgcgcg gatatcctcg cccgccctga tttcacaatg ttcgccgacg atcccaccct
       61 cgacgatgca gctccccgaa gcctcgatac gtgcgttgaa cgcggaaccc gatacggcga
      121 catcgcgccc cgccttcacg gaaaacccct ccgtcaccga accgcggacg accaccgagc
      181 cgatgaagtc gatatcgccg gaaagtatgt cgacgtcgcc gggtacctca agcacgggga
      241 gcacatccag ctcgccgccg cggaagacgg gctgccccac gtcccgggcg gtaagcactt
      301 cggtcatgcc gtcttcctcc gaaacgacca cgccctcccc gaacttcacc cggaagggcg
      361 cggggcggcg gggaggaagc ggctgaccgt agatgtccgc cccttcaacg ccctcctgcg
      421 gggggtgaac ccgggcgatg atgtcgccgg tatggacgat cggaatactc cacaagtttt
      481 tccagtcgat ggcgcgctcg tcggcttcgt cgaccagttt gaccttttcg aagggcacga
      541 ggaactcgat cttcccttcc acgggatcga ccgttgctgt tccggaggcg accaaggcct
      601 ccgtccccct cacttcttcg cctttgttgc atttttcaat gatcgtacgg atttttcccg
      661 gatcgagcaa acaaagcccc gcctcctcca gagcttcgat aatctccgca gcgtccagag
      721 gaatcccggc gccgacctgg ggataggttt cgagccatgc ggacagacga tcatcggaga
      781 ccctgagctc ccatgacgcg tcgcggctct ccgccgcggg caacgcgcct gtctccttcg
      841 tcccggagag aaaatggtcg atctccgtca ccagtctctc caggttggct atccgcagtt
      901 cgtccatgcg ttttctcctt cccgctccgg cgctccgacc tacggaacgg catgcatcga
      961 gcaaaagacc atgtcgacga cgccgttttc actgcaaatg gggtagagcg cgtactccag
     1021 cggcatgtcg ccgtgcggcg ccggaaaaac aagaacgccg cgcttcgatt tcccggctgc
     1081 gaacgtctgc gccaaatgtt catcgaacgc ggcaattcgc tcctccggaa tgccgagcgc
     1141 ctcccatgaa cgaccggtca tctcctccgg tgttctttca aagagcgccg cccctttctt
     1201 tccggaaaaa cagaattttc tcatgcgatc aaacacgtag aggtagtcgg gcgagctctc
     1261 cagaataccg ccgagaatct tcgcctgcag ggcgcagtcg aaggtgacca gatggagctg
     1321 ttcctcgaga cgctgtttct cggtgatatc gtgaagaata gaaaagagaa aggtcttccc
     1381 ctgaaacgga gtcggcgaag caaagacctc cactgtgcgg atgtcgccgt tcttgagacg
     1441 atgctggaaa acgaaccggt tcctgagccg cgccgtcgcc tcttccatgt tcttgcgcag
     1501 atcttccgcc ggaagcagat taaaatctcg gttcgtcaag gcgagcatct cttctctgga
     1561 ataaccgtag agggcaaggg cggcggcgtt acagtcgcat acggcgcccg tttccgggtc
     1621 gacgaggagc ataggggcca tggtctcctt gaaggcggcg gcataccggc gctcgctttc
     1681 caaaagctgc tcctcggtgt ggcgatcctg cgtcacatcg cgcccaagga gcagaagacc
     1741 gtcggaggag acatcctcga aatgccacgg ccgaaccgtc cactccacac gaagcgatct
     1801 tccctcagcc gcgcacacgc aggtttccag ccggacctcc gtcgcaccgt cccttcggca
     1861 ggcatccatc ttcccgatgg catcttcacg atcctcttct ccgggacaaa aaacgctccg
     1921 gaaatcaaag ctccccgaga gcgcttcgtc ggccgagatg ccggacaagc gctccgcggc
     1981 ggcgttccag aagacgactc ttccccgttc gtcgagcagc acgatccaga catccacgct
     2041 tccgaagact ttttccgtaa accgttcgtt ctcccgccgc tccctttccg cggcaacctg
     2101 actggtcgca tccgttatgg ccatacaaaa cgtgccgttt tcgagagaga acgcaagaac
     2161 gtcaaaaacc ttgcttaatt tttcggagga aaacatgaag gaaaaggcct gttgttcttt
     2221 gagagcacgg tcaaaaaggt ccagggcggg aatctccttg ccttcgggca tgaagaactc
     2281 ctttccgctc ctgcccgccg cgctctccgg ggctatgccg aaaatcctgc aaaaagcggg
     2341 gttgacgctc tccagcagaa aatctaccgg tatcccccgc gcattgaaga gcatacggca
     2401 gacaaccact gcgcgtgagg tagaacggaa aacgatatcg tttgaatgca actcttcagg
     2461 cttcaaaccc tccatagttc ctgagtcttt cccgtcggga cgcactccgg cttcggaggc
     2521 tatcgactcg atgctcttca gcagctcctc gttccacttc tccatcacgc actcctcctt
     2581 ccggctgttc ccgctctcag aaaccgcctc ttgcgatcac gccgcggtct ttgtccataa
     2641 agaaagacac atggaattcc tgctgcttga ccaccagcgt ttgtccccaa attttcaggg
     2701 tgacgttggg gtagactctg tcgcggatgc ggatgcgggg catgatgcgc ttttccgcta
     2761 tggacgcgcg tttttcatcg tcgatccttg cggccagctc cgctacggaa ctcttcatct
     2821 tgccgtggta ttga
//
LOCUS       DAOTIL010000297         2833 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminobacteriaceae bacterium isolate AMR_MDS_3026
            NODE_41947_length_2833_cov_3.183225, whole genome shotgun sequence.
ACCESSION   DAOTIL010000297 DAOTIL010000000
VERSION     DAOTIL010000297.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868050
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminobacteriaceae bacterium (wastewater metagenome)
  ORGANISM  Aminobacteriaceae bacterium
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae.
REFERENCE   1  (bases 1 to 2833)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2833)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:19:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,224
            CDSs (total)                      :: 3,198
            Genes (coding)                    :: 3,184
            CDSs (with protein)               :: 3,184
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2833
                     /organism="Aminobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_41947_length_2833_cov_3.183225"
                     /isolate="AMR_MDS_3026"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:3034609"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..504)
                     /locus_tag="P5201_07065"
     CDS             complement(<1..504)
                     /locus_tag="P5201_07065"
                     /inference="COORDINATES: protein motif:HMM:NF012695.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NTP transferase domain-containing protein"
                     /protein_id="HRV98330.1"
                     /translation="MSASRCVTGALVLAAGKGTRMKSDLPKVLLPVLDQPVLYYILDT
                     LRRCMASSKGCVQQLAVVVGHRGETVQEYLGSNWPEALPLWQHEQLGTGHAVQIAKEW
                     WTGLDHLLVLPGDVPLLTEASLSGLVEEHIVSGADCSFISFHASDPSGYGRVVREGKE
                     ISIVEDKD"
     gene            complement(585..658)
                     /locus_tag="P5201_07070"
     tRNA            complement(585..658)
                     /locus_tag="P5201_07070"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(624..626),aa:Gln,seq:ttg)
     gene            850..1356
                     /gene="mrtS"
                     /locus_tag="P5201_07075"
     CDS             850..1356
                     /gene="mrtS"
                     /locus_tag="P5201_07075"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF040589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SYNERG-CTERM system CAAX-type protease"
                     /protein_id="HRV98331.1"
                     /translation="MTAFIVFAFFLYFPYAWCALRGESPEKYALLWRLSPSSARELLL
                     VSGGALVPLTFISLFFFARYLPPWHVERILPGILSGLAAAVAEETFFRGWLQTLFAEK
                     YSEWKSILFASFFFGLAHIFQSPAAMLAFFPGIIMGLLRSRHGTVFSAILFHWSGNIW
                     SIWFYPHL"
     gene            1386..2480
                     /locus_tag="P5201_07080"
     CDS             1386..2480
                     /locus_tag="P5201_07080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013048141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRV98332.1"
                     /translation="MFSTTLRKLRAARLALLLTLFFACSGEAAPILYIVRIPLVLGEE
                     AQAVLPDGKTIPLGKVASLPTSSRWPGYTASKWAPPGSVAASAVNAVHLTLSVEKEKG
                     RTVSILPRHTVAPAAGEQSFIALDSPAGTGFFGGWAPPVATPVLVRRNDGALVPLEER
                     GLPREGDTLIFEVSESESPYLIDIENRPGGRVLGWYESGPRLLARVIRPLKGVGRFGG
                     TEFQNIGRIRANHSGVIDVSTTPRGVVGGFQILPFLHSKSQEMSSAWQLTQWLIIASP
                     TDRPLPGTAPLFSSNLVPGSQMTDVLWDMWSTYGRKPLVLCRRGGGAWQRLPEASGRN
                     DSALGDLTHLRIYSPFTEEPQKGFVPGTGK"
     gene            complement(2540..>2833)
                     /locus_tag="P5201_07085"
     CDS             complement(2540..>2833)
                     /locus_tag="P5201_07085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009200936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0097367 - carbohydrate derivative binding
                     [Evidence IEA];
                     GO_process: GO:1901135 - carbohydrate derivative metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HRV98333.1"
                     /translation="PRVPVVVIAPKDDLHEKTFSNILEAKARKAPVIAIASEGDRSLD
                     SAADNVFNVPSTLPMLSPFLTVVPLQLFAFFVAKLLGCDIDQPRNLAKSVTVE"
BASE COUNT          537 a          830 c          841 g          625 t
ORIGIN      
        1 gtccttgtct tcgacgatcg agatctcttt gccttcccgc acaactcttc cgtagccgga
       61 gggatcggac gcgtggaagc tgatgaagga acagtccgct cctgaaacga tatgctcctc
      121 gacaagaccg gaaaggctgg cctccgtaag aagcggcacg tctcccggga gaacgaggag
      181 atgatcaagg ccggtccacc actccttggc tatctgtaca gcatgtccgg tcccgagctg
      241 ctcatgctgc cacaaaggga gcgcctcggg ccaattcgaa ccaagatact cctgaaccgt
      301 ctcccctctg tggccgacaa cgaccgcgag ctgctgcaca caccccttgg aggatgccat
      361 gcaccgccgg agcgtgtcga ggatataata gagcaccggc tgatccagaa ccggaagcag
      421 gactttcgga agatcgcttt tcatcctggt cccctttccg gcggcgagta ccagcgcccc
      481 ggtgacacaa cgagaagcgg acacgtgaat caacaccttt acattgaatt ttccacaaca
      541 caaaaaaagg ggatccgctt caggccccct ctgcccactg tacatggctg gggtggctgg
      601 attcgaacca gcgatggcgg atccaaagtc cgctgccttg ccgcttggct acaccccatc
      661 ctcaacaatc tcaatatagt aacagaaagc cagatggtgt caaggcttac gaaattgcat
      721 caaatgagca gcagatgcgt tttcccggtg gaaggggggt gaaggccgga cgatgcaacg
      781 caagaaacaa tcccgcagaa tgctcaagtg cgtgcgtata aagaagcggt gtgctatgat
      841 agccttcaaa tgacggcatt catcgtgttc gctttttttc tttactttcc gtatgcgtgg
      901 tgcgctctgc ggggagaatc tccggaaaag tacgctctct tgtggaggct ttcgccttcc
      961 tcggcccggg aacttcttct tgtttcaggg ggcgctcttg ttccgttgac tttcatttcg
     1021 ctgttctttt ttgcccgcta tctccctccc tggcatgtgg aacgaatcct tccgggcatc
     1081 ctctccggcc tggcggcggc ggtggctgag gaaacatttt tccggggctg gcttcagacg
     1141 ctgtttgccg agaagtattc cgagtggaag agcatcctct tcgcctcctt ttttttcggg
     1201 cttgcgcaca tcttccagag tccggcggcg atgctcgcct tctttccggg catcatcatg
     1261 ggacttttgc gaagcaggca cggaacggtg ttttcggcaa ttttgtttca ctggtccggc
     1321 aatatatggt cgatctggtt ttatccgcat ctttagcggc gcctacaaaa agggaggtat
     1381 gatccatgtt cagcacgaca ctccggaagc tgcgtgcggc gcgtctcgca cttcttctca
     1441 ccctcttctt cgcgtgttcg ggagaggccg cgccgattct ctatatcgtt cgcatccctc
     1501 ttgttcttgg cgaggaggcg caggcggttt tgcccgacgg aaagacgatt ccgctcggaa
     1561 aggttgcctc gctgccgacg tcgagccgct ggccggggta cactgcctcg aaatgggcgc
     1621 cccccgggag cgtcgccgct tcggctgtga acgcagtgca cctgacgctc tccgtcgaga
     1681 aagagaaagg gcggactgtg agcattctcc cccggcatac cgtggctccc gcagcgggcg
     1741 agcagtcgtt tatcgcgctc gactccccgg cgggaacggg tttcttcggg ggctgggctc
     1801 ctcctgtggc gacgccggtt ctcgtccggc ggaatgacgg cgcactcgtg ccattggagg
     1861 agcgcggcct tccccgcgaa ggggatacgc tcattttcga agtttcggag tcggagagcc
     1921 cgtatttgat cgacatcgag aaccgtccgg gcggccgcgt cctcggatgg tacgaatcag
     1981 gcccgaggct gctcgcccga gtaatccgcc ctctgaaggg cgtgggacgt ttcggaggca
     2041 ccgagttcca gaatatcgga agaatacggg cgaatcattc tggagttatc gacgtcagca
     2101 cgacgccgcg aggtgttgtt ggaggttttc agattctccc gtttctgcat tcgaagtccc
     2161 aggagatgtc gtccgcctgg cagctgacgc agtggttgat tatcgcgtcg ccgaccgacc
     2221 ggccgcttcc cgggactgcg ccgcttttct cctccaacct cgtgccggga agccagatga
     2281 cggacgtcct ctgggacatg tggtcgacgt acgggcgcaa gccgctcgtc ctctgccgtc
     2341 gggggggagg agcgtggcag aggcttccgg aggcctcggg gagaaacgac tccgctctcg
     2401 gagacctgac gcacctgcgt atttattccc catttactga agaacctcag aagggcttcg
     2461 tgcccgggac gggaaagtaa aagaacggcg gcctgatcag gccgccgttc ttttttctgc
     2521 gcttcggcgg gagtcccgcc tactctactg tgacgctttt cgcgaggttc ctgggctggt
     2581 cgatgtcgca gccaagaagc ttcgccacga agaatgcgaa gagctgcagc ggcaccaccg
     2641 tgaggaacgg cgagagcatc ggcaacgtcg acggcacgtt gaagacgttg tccgcagccg
     2701 agtcgaggct ccggtcgccc tcggaggcta tggcgatcac cggggccttt ctcgccttcg
     2761 cttcgaggat gttggaaaag gtcttctcat gcagatcatc cttcggcgca atgacgacga
     2821 caggcacacg agg
//
LOCUS       DAOTIL010000298         2830 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminobacteriaceae bacterium isolate AMR_MDS_3026
            NODE_42024_length_2830_cov_3.436757, whole genome shotgun sequence.
ACCESSION   DAOTIL010000298 DAOTIL010000000
VERSION     DAOTIL010000298.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868050
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminobacteriaceae bacterium (wastewater metagenome)
  ORGANISM  Aminobacteriaceae bacterium
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae.
REFERENCE   1  (bases 1 to 2830)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2830)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:19:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,224
            CDSs (total)                      :: 3,198
            Genes (coding)                    :: 3,184
            CDSs (with protein)               :: 3,184
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2830
                     /organism="Aminobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_42024_length_2830_cov_3.436757"
                     /isolate="AMR_MDS_3026"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:3034609"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..459
                     /locus_tag="P5201_07090"
     CDS             <1..459
                     /locus_tag="P5201_07090"
                     /inference="COORDINATES: protein motif:HMM:NF015431.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HRV98334.1"
                     /translation="PFSRDPALTYRSLGEDRLVLAAPQGHPWASTGQASFTDAERRTL
                     IRFAGDCPLATYVDEFLLRNRIVFGEQVETDEIELAKNLAEAGVGVLITSSLSVQRSI
                     ASGRLAPVMLEGMDDMSWEIQCVYSSSKGLSYPGWEMAKRLETTCRELLH"
     gene            complement(510..2369)
                     /gene="pepF"
                     /locus_tag="P5201_07095"
     CDS             complement(510..2369)
                     /gene="pepF"
                     /locus_tag="P5201_07095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005661094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligoendopeptidase F"
                     /protein_id="HRV98335.1"
                     /translation="MIRTQSGNGAKKAAFTEISFGGKVPPRCDIPVEYTWDLSALQPS
                     DEAWEESFRRAGEEMQQLASYNGKLFDSPETLLEFLRLEEKASERMGKLYAYATMKSH
                     ENTSEALYQGMADRAGMLYSSFAAVCSFFNPEVLAAGEEKVRDFIARCTGLALYRHFF
                     DDLLRSKEHVLSPREEELLARSSEMARTAETAFSLFTNADMTFPSIVDEKGEETELSE
                     ERYYLLSRSRDRRVRKEAFEGLLGTYGKYRNSLGALYSGSVKGDIFYATARKYQSSLD
                     ASLDADNIPSSVYDNVVSTVNANLDAMHDYMRLRKKALALDELHMYDLNVPLADEPDA
                     AIPYGKAVEMVAEGLAPLGEEYIDVLRNGFSTRWIDVYENQGKHKGAYSWGSYGAHPF
                     VLLNYSGTLRDVFTIAHEMGHSLHTWHSQERQPQVYADYTIFLAEVASTVNESLLLES
                     LLEKASREEKIYLLNHYLDQVRTTVYRQAMFAEFERDMHAAAEQGAPLTADSLCGVWR
                     ELNERYHGPAMTVDADIEVEWARIPHFYSAFYVYKYVTGFTAAAMLTRQLRKGSAEER
                     ERYLRFLSRGSSAYSLDILKEAGVDMTTSTPLEETVANFREKTKQLSGLLGID"
     gene            2516..>2830
                     /locus_tag="P5201_07100"
     CDS             2516..>2830
                     /locus_tag="P5201_07100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014163846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TAXI family TRAP transporter solute-binding
                     subunit"
                     /protein_id="HRV98336.1"
                     /translation="MKRRVLLLLALALVVSFSGTAFAAKEGWPDQLRFMAGPPGGNWF
                     ALGGALADMWSKEVLQTTSITGGGVANVVNTDNTKGDLGFSVTSFVGAAGKGEDPFKA
                     PAK"
BASE COUNT          590 a          862 c          788 g          590 t
ORIGIN      
        1 cctttttcaa gggatccggc gctcacatac cgttctctcg gcgaggacag actcgttctg
       61 gccgcccccc aaggacaccc ctgggcttcg accggtcagg cgtcgttcac cgacgcggag
      121 cgccgcaccc tgatccgttt tgcgggcgac tgccctcttg ctacctatgt ggacgaattt
      181 ttgctccgca acaggattgt cttcggcgaa caagttgaaa ccgacgaaat cgagttggcg
      241 aagaatctgg ccgaggccgg cgtcggggtc ctcatcacca gctcactctc ggtgcagaga
      301 agcattgctt caggccgcct cgcgcccgtc atgctcgaag gcatggacga catgtcgtgg
      361 gagatccagt gcgtctactc atcctccaaa ggcctctcct accccggctg ggagatggca
      421 aaacgcctgg agacaacctg ccgcgaactg ctgcactgaa gaacgccact ctatgcaaga
      481 ggggctcctt cggagcccct cttttgttct caatcgatcc ccagaagccc ggagagctgc
      541 tttgtctttt ccctgaagtt cgccacggtc tcctcaagcg gtgtggaggt ggtcatgtcc
      601 acacctgcct ccttcagaat atccagagaa tacgcggaac tccctctcga aaggaaacgc
      661 aggtagcgct cgcgctcttc ggcgcttccc ttccggagtt ggcgcgtgag catcgccgca
      721 gcggtaaaac cggttacgta cttgtagacg tagaacgcgg agtagaaatg agggatacgc
      781 gcccactcca cctcgatgtc cgcgtcgacc gtcatggcgg gcccgtgata gcgctcgttc
      841 agctcccgcc acacgccgca gagagaatcc gcggtcaggg gcgctccctg ttccgctgcg
      901 gcgtgcatgt cccgctcgaa ctccgcgaac atcgcctgac gatagacggt agtgcgcacc
      961 tgatcaaggt agtgattcag aagatagatt ttttcctccc gcgaggcctt ctccagcaag
     1021 gattccagca gcagcgactc gtttaccgtg gaggccacct ccgcgaggaa gatcgtgtag
     1081 tcggcgtaca cctggggctg cctctcctgc gagtgccacg tatgcagcga gtgccccatc
     1141 tcgtgggcga tggtgaagac gtcgcggagc gtgccgctgt agttcagcag cacgaacggg
     1201 tgcgctccgt agcttcccca cgaatacgcg cccttatgtt tcccctggtt ctcatacaca
     1261 tcgatccacc gggtggagaa tccgtttcgg aggacgtcaa tatactcctc tccgagaggg
     1321 gcaaggccct cggccaccat ctcgaccgcc tttccgtacg gtatggccgc gtcgggttcg
     1381 tccgcaagcg gtacgttgag gtcgtacatg tgcagctcgt ccagggcaag cgccttcttc
     1441 ctgagacgca tgtagtcgtg catcgcatcc aggttggcgt tcaccgtcga aacgacgttg
     1501 tcgtagaccg aggaggggat gttgtccgcg tcgagcgaag catccagcga ggactgatac
     1561 ttccgcgccg tcgcatagaa gatatccccc ttcacactcc cggaatagag cgcgccgagc
     1621 gagttccggt acttcccgta ggtccccagc agaccttcga acgcctcttt gcgcacgcgg
     1681 cgatcccgcg aacggctcag aaggtagtac cgctcctctg aaagctctgt ttcctccccc
     1741 ttctcgtcga cgatcgacgg gaaggtcata tcggcgttcg taaaaagcga aaacgctgtc
     1801 tccgccgtac gggccatttc ggaagaacgc gccagaagtt cctcttcgcg cggcgacagc
     1861 acatgttcct tcgagcgcag aagatcgtcg aagaagtggc ggtagagcgc aagaccggtg
     1921 caacgcgcga tgaagtcgcg caccttctcc tctccggcgg caaggacttc cgggttgaag
     1981 aaagagcaga cggcggcgaa ggaggagtac agcatcccgg ccctgtccgc catcccctga
     2041 tagagcgcct cggaggtatt ctcgtggctc ttcatcgtcg cgtaggcata cagctttccc
     2101 atacgttcgg acgccttctc ctccaggcgc agaaattcca aaagcgtctc gggagaatca
     2161 aagagtttcc cattgtagga ggcaagctgc tgcatctcct caccggcgcg acggaaagac
     2221 tcctcccatg cctcgtcgga aggctgaagc gcagagagat cccaagtata ctcgacagga
     2281 atatcacagc gaggagggac ctttcctccg aaagatattt ctgtaaaagc ggcctttttt
     2341 gctccattgc cagactgagt acgaatcata acgaaactcc tttcacaaac aaggaaattt
     2401 tgaaaaataa tgttcaaacg gttttattga catgccgccc cgaagactat atcatttttc
     2461 cgtctgtttc tcagggaagt tctactcaaa atctcatcaa aggagtgtgt aaaaaatgaa
     2521 aagacgcgtt cttctgcttc tcgccctcgc acttgtggtc tccttttccg gaacggcctt
     2581 cgcggcaaag gaagggtggc ccgatcagct tcgattcatg gcgggacctc cgggcggcaa
     2641 ctggttcgcc ctcggcggcg cacttgccga tatgtggtcg aaggaagtcc tccagaccac
     2701 cagcatcacc ggcggcggcg ttgccaacgt ggtgaatact gacaatacca agggcgatct
     2761 cgggttttcg gtaacatcgt tcgtcggcgc ggcgggcaag ggagaggatc ccttcaaagc
     2821 ccccgcgaaa
//
LOCUS       DAOTIL010000299         2828 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminobacteriaceae bacterium isolate AMR_MDS_3026
            NODE_42072_length_2828_cov_3.314821, whole genome shotgun sequence.
ACCESSION   DAOTIL010000299 DAOTIL010000000
VERSION     DAOTIL010000299.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868050
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminobacteriaceae bacterium (wastewater metagenome)
  ORGANISM  Aminobacteriaceae bacterium
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae.
REFERENCE   1  (bases 1 to 2828)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2828)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:19:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,224
            CDSs (total)                      :: 3,198
            Genes (coding)                    :: 3,184
            CDSs (with protein)               :: 3,184
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2828
                     /organism="Aminobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_42072_length_2828_cov_3.314821"
                     /isolate="AMR_MDS_3026"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:3034609"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(124..2217)
                     /locus_tag="P5201_07105"
     CDS             complement(124..2217)
                     /locus_tag="P5201_07105"
                     /inference="COORDINATES: protein motif:HMM:NF021619.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PocR ligand-binding domain-containing protein"
                     /protein_id="HRV98337.1"
                     /translation="MDIDFVTDSSYEIRFEDMFDLADIQRLQDEFSDATGVASVVTRP
                     DGTPLTRPANFTRFCSKVVRGAEKGCANCFRSDALIGRYNPKGPTIQRCLSGGLWDAG
                     AGISVGGKHVANWLIGQVRDESQNEEAIRAYAREIGADEDEAAAAFREVPAMSRDRFQ
                     KIARMLFTLVQQLSELAYQNVRQTLFIAEKNRAETIRKVQHELAEVMATAGSEIEVFD
                     MARRELAGLMETKNFLIVDYDETTRMLSTRMHLGVDEKKLPVRWSADKALTGIMMAAR
                     KAMHFSRTEIIRLVDEGLIEFVGDRAETWVGVPLQNEKKVFGAMILQSYTDPYAYDDW
                     CVEILQSVGNQLSVYLEKKRAEGALRESEEMFRAFFEQNSVGFAMINTDGSWLRFNDK
                     LCEMTGYSREEMQEMNWRDFTPPEEQQRERPLLNDLISGKIDSYTIEKRCIRRDGSTI
                     DIFVLTKPLRRPDGEFRCFASIIQDISERKKSERELARHQEAIIGSMAILAERRDHGT
                     GEHIQRTKFFVQRLLELSGGERFYPLEHLPLLAQAAILHDIGKVGVPDSLLLKPGKLT
                     PEEFEVVKQHTVIGGDVLAAAARILGEAPFISYARQIIEYHHEKWDGTGYPHGLKGEE
                     IPFIARIMAIADVYDALVSERPYKGPIDHEEAVAIIVEGAGTHFDPVLVEAFLACADE
                     FKSISQSGDTPVPGR"
     gene            complement(2483..>2828)
                     /locus_tag="P5201_07110"
     CDS             complement(2483..>2828)
                     /locus_tag="P5201_07110"
                     /inference="COORDINATES: protein motif:HMM:NF024110.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HRV98338.1"
                     /translation="AFVPAVVLLSTASTFLGLFIAAVAGEVFEAQTFSNFFRFPMIFL
                     CGLFFPIERLPVFLRPLSYALPPTYGVDMLRGAVGGVHTMPYLLNLAALCVFCAGLFA
                     LSLRTIRRSWIA"
BASE COUNT          553 a          844 c          845 g          586 t
ORIGIN      
        1 gagaccgaat gcgaagagta cattctcaat ttccatgtgg cgacgacctc cttgatatga
       61 tgtcacgtgt acccggacac acaataccga aacgaagcgg cggcgtcaag cgagggcatt
      121 tctttagcgg ccgggtacgg gggtatctcc ggattgggag atacttttaa attcgtcggc
      181 gcaggccaaa aaggcctcga cgaggacggg gtcgaaatgg gttcccgccc cctcgacgat
      241 gatcgccaca gcctcctcgt ggtcaatggg gcccttgtag gggcgttcgc tcacgagcgc
      301 gtcgtagacg tcggcgatcg ccatgatcct cgcgataaag gggatctcct ctcctttcag
      361 cccgtgcgga taccccgtgc cgtcccactt ctcatgatga tactcgatga tctggcgcgc
      421 atagctgata aacggcgcct cgccgagtat ccttgccgcg gccgcgagca cgtcgccgcc
      481 gatcacggta tgctgtttga cgacctcgaa ctcctcgggc gtcagcttgc cgggcttcag
      541 cagcaggctg tcggggacgc cgaccttgcc gatgtcgtgc agaatggccg cctgcgccag
      601 aagcggaagg tgctcaaggg ggtagaagcg ctcgccccct gaaagttcga gcagccgctg
      661 aacgaaaaat ttcgtgcgct gaatgtgctc ccctgtgccg tggtcgcggc gctccgccag
      721 gatggccatg ctgccgatga tcgcctcctg gtggcgtgcc agctcccgtt cgcttttttt
      781 gcgctccgag atgtcctgaa tgatggaggc gaagcaccgg aattccccgt cggggcgtcg
      841 caggggtttg gtaagcacga agatgtcgat cgtcgaaccg tcccgacgga tacagcgttt
      901 ctcgatcgtg tacgaatcga tctttccgga gatcagatcg ttcagcaaag ggcgttcgcg
      961 ctgctgttcc tcgggcggag tgaaatcgcg ccaattcatc tcttgcattt cctcccggga
     1021 gtagcccgtc atctcgcaca gcttgtcgtt gaagcgcagc cacgaaccgt ccgtgttgat
     1081 catggcaaag ccgacgctgt tctgctcgaa gaatgccctg aacatctcct cgctctcgcg
     1141 gagcgctccc tcggcgcgtt tcttctccag gtagacgctg agctggttcc ctaccgattg
     1201 cagaatctcg acgcaccagt cgtcgtacgc atacgggtct gtgtaactct gaaggatcat
     1261 ggcgccgaag acttttttct cgttctgcag cggcacgccg acccatgttt cggcccgatc
     1321 accgacgaat tcaatgagcc cctcgtcgac gagccgtata atctcagttc gggaaaagtg
     1381 catcgccttt ctggcggcca tcataatgcc cgtcagcgct ttgtccgcgg accatcgtac
     1441 cggcagcttt ttttcatcca cgcccagatg catacgcgtc gagagcattc ttgtcgtttc
     1501 gtcgtaatca acgatcaaaa aattcttcgt ctccatcagc ccggcgagct ctcttcgagc
     1561 catatcgaac acctcgatct ccgagccggc cgtcgccatg acttcggcaa gttcgtgctg
     1621 taccttcctg atggtttccg cgcggttttt ctccgcgatg aaaagcgtct gccggacgtt
     1681 ttggtaggcg agttcggaga gctgttgtac aagcgtgaag agcattcgtg caattttctg
     1741 gaatcggtcc ctggacatgg cgggaacctc gcggaaggca gccgccgctt cgtcctcgtc
     1801 cgcgccgatc tcgcgggcgt aggcgcgtat cgcctcttcg ttctggctct cgtcgcggac
     1861 ctgccctatc agccagttgg cgacgtgctt ccccccgacg gagattcctg cgcccgcgtc
     1921 ccagaggcct ccgctgaggc agcgctgaat cgtcggtccc ttggggttgt accgcccgat
     1981 gagcgcgtcc gaacggaagc agttggcgca ccccttttcg gcgccgcgca caaccttgct
     2041 gcagaagcgg gtgaagttgg cagggcgcgt caacggcgtc ccgtccggcc gcgtgacgac
     2101 cgaggcgacg cccgtcgcgt ccgagaactc gtcctgcaga cgctggatgt cggcgaggtc
     2161 gaacatgtcc tcgaagcgga tttcgtaaga gctgtccgtt acaaaatcga tgtccatgac
     2221 gaaaacctcc ggagatagtc gtttattcac gctacttcct tccgggagag atgcggcaat
     2281 tccccaaaga gcgcgcagag agagttttct gcggcgcccc gtatgcgcat acacaatctt
     2341 ccgatggttt gtattataga ctgtcaaggg gtatttcgca ccttttgcct tccaaggcga
     2401 gcgcaagaaa gagtgtgcgc ttccaaaaaa gaggaggtgc aagagaatca cgcagttatt
     2461 cgagagaaaa ggaggcggcg cgctacgcta tccagctcct ccggatggtc cgcaggctga
     2521 gggcgaagag ccccgcgcag aagacgcaga gcgcggcgag gttcaggagg tacggcatgg
     2581 tatggacgcc gccgacggcc cctctgagca tgtccacccc gtacgtcgga gggagcgcgt
     2641 aggagagcgg gcgcaagaag acgggcagcc tctctatggg gaagaagagc ccgcagagga
     2701 agatcatcgg gaagcggaag aagttggaaa acgtctgcgc ctcgaagact tcgccggcga
     2761 ccgccgcgat gaagagcccc agaaacgtgg atgctgtcga cagcagcacg acagccggaa
     2821 cgaacgct
//
LOCUS       DAOTIL010000300         2820 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminobacteriaceae bacterium isolate AMR_MDS_3026
            NODE_42234_length_2820_cov_3.084629, whole genome shotgun sequence.
ACCESSION   DAOTIL010000300 DAOTIL010000000
VERSION     DAOTIL010000300.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868050
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminobacteriaceae bacterium (wastewater metagenome)
  ORGANISM  Aminobacteriaceae bacterium
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae.
REFERENCE   1  (bases 1 to 2820)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2820)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:19:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,224
            CDSs (total)                      :: 3,198
            Genes (coding)                    :: 3,184
            CDSs (with protein)               :: 3,184
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2820
                     /organism="Aminobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_42234_length_2820_cov_3.084629"
                     /isolate="AMR_MDS_3026"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:3034609"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..324
                     /locus_tag="P5201_07115"
     CDS             <1..324
                     /locus_tag="P5201_07115"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="YbjN domain-containing protein"
                     /protein_id="HRV98339.1"
                     /translation="RVEDLVSFSFGSATIQVTVLPWHSGDVLVKVFSYLAENVDKNKV
                     AAEFLRLNADLPLGGFSLVFDNTVMLSCSLPGAKLDKSELLGAVQTVAVYADQYDEIL
                     KDLYR"
     gene            358..1146
                     /locus_tag="P5201_07120"
     CDS             358..1146
                     /locus_tag="P5201_07120"
                     /inference="COORDINATES: protein motif:HMM:NF023899.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="E3 ubiquitin ligase family protein"
                     /protein_id="HRV98340.1"
                     /translation="MNFVALGIGGLMIAGGAYLSFIQKKKSEGLVTELKFMKAVSLGE
                     LKESWQSLSDEGMADGFRDMVETNGKAGTDGEMRAPYSNTPCAYFEATVTREFEKEER
                     STDKDGKVQSTRTRSSEVVSSRKSPSPLYIADGDIRVGIDMDGATLHLKDGEDRFEPY
                     ESNKTYTFFGVQFSAPSGVRTLGFRYKEKLIPLGHPLYVVGEARHSAGALRIGKPSEK
                     GKPFIVSVKSEDEVVSGMQGSVKFVFYLGIALMIGGAAVAVFFR"
     gene            1178..2401
                     /locus_tag="P5201_07125"
     CDS             1178..2401
                     /locus_tag="P5201_07125"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRV98341.1"
                     /translation="MKKGIVARVLVAVLLVLAVVSTGFAADPKYSKSSTFGPMITGGV
                     TDEFLQMVREDPDIKKGKPLRLGKDITDDDLKKVATLADVLTGVLIERNDTITDLSPL
                     SSLSGLTNMKLDGLKGVKTLAPVGALVKMKQLEIRQVEYPDLDFLTGMNDLESLIFFM
                     QPKQIEDISPLKGKAKLKTLRLYGGAGQDGISDISVLEELTELEDLSLYMTKVEDLSP
                     ISGLTKLKRLDLYGIPATDMSPVENLTELRYIWIYATKFEDYSPLANLTKLEEFRAGI
                     SKLDTLEYLESMPKLKIVSMLREDIQDFSPLAKCPNLTEVNLEAVKGVIDLSVFAEHK
                     KLKNLFVRNAEVKNAEAIVALPRLEWLILIDTKGIADVSIFKDLPKLNRVSVKEGQFP
                     AEQVEALGKKASVQK"
BASE COUNT          648 a          729 c          866 g          577 t
ORIGIN      
        1 cgggtggagg acctcgtctc cttctccttt ggcagcgcga cgatccaggt gacggtgctt
       61 ccgtggcata gcggggatgt tctggtgaag gtcttttcct acttggcaga gaatgtcgac
      121 aagaacaagg ttgcggcaga attcctgcga ctgaatgccg acctgccgct cggcggcttc
      181 tcgcttgttt tcgacaacac cgtgatgctc tcctgttctc tgcccggagc gaagttggac
      241 aagagcgaac tgctgggggc ggtgcagacg gtcgccgtct acgcggacca gtacgacgaa
      301 atcctgaagg atctgtacag gtagttttcc ctgaagggga ggaaaggagg aggttgcatg
      361 aatttcgtgg ctcttggtat cggcggtctg atgatcgcag ggggcgcgta tctctcgttc
      421 atccagaaga agaagtctga agggcttgtg accgagctga agtttatgaa ggccgtttcg
      481 ctcggcgagc tgaaggagtc gtggcagtcg ctctccgacg agggaatggc cgacggattc
      541 cgagacatgg tcgaaaccaa cggcaaggcc gggactgacg gagagatgcg tgcgccgtac
      601 agcaacactc cgtgcgccta ctttgaagcg acggtcactc gtgaattcga gaaggaagag
      661 cgttcgaccg acaaggacgg aaaggtgcag agcacgcgta ccagaagttc cgaggtcgtc
      721 tcgtccagaa agagcccttc gccgctctac attgctgacg gagacatccg cgtcggcatc
      781 gacatggacg gagcgacgct tcatctcaag gacggagagg accggttcga accgtacgag
      841 agcaacaaga cgtatacgtt cttcggggtg cagttctccg ctccctcggg agttcggacg
      901 ctcggcttcc gctacaagga gaagctcatt cctcttggac acccactgta cgtcgtcggg
      961 gaggcgcgcc attctgcggg agcgctgcgc atcggcaagc cttctgaaaa ggggaagccc
     1021 ttcatcgtct cggtgaagtc ggaggacgag gttgtctccg ggatgcaggg gtctgtgaag
     1081 ttcgtgttct atctggggat cgcgctgatg atcggcgggg cggctgtggc cgtcttcttc
     1141 cggtagcgcg aaacgctctg cgaggagggg aaattcgatg aaaaagggaa tagtggcacg
     1201 cgttctggtt gctgtattgc tggtactggc agtggtttcg acggggtttg ccgcagaccc
     1261 caagtactcg aagagctcga cgttcggtcc catgattacc ggcggtgtga cggacgagtt
     1321 cctccagatg gttcgggagg acccggacat taaaaagggg aagccgctcc gtctcgggaa
     1381 ggacataacc gacgacgatc tgaagaaagt cgcgacgctc gccgatgtgc tgacaggagt
     1441 tctgatcgag aggaacgaca cgatcaccga cctttcgccg ctctcctcgc tctccgggct
     1501 gaccaacatg aagctcgacg gcctcaaggg cgtcaagacg ctcgcgccgg tcggagcgct
     1561 cgtgaagatg aaacagcttg aaatacgcca ggtcgagtac cctgatctgg atttccttac
     1621 cgggatgaac gatctcgaat cgctcatatt ctttatgcag cccaaacaga tcgaggacat
     1681 ttcgccgctg aagggaaagg caaagctgaa gacgctgcgc ctctacggag gggccgggca
     1741 ggatggcata tcggacatct ccgtgctgga ggagctgacg gagcttgagg acctcagcct
     1801 ctacatgacc aaggtggagg acctctcccc tatctccggc ctgacgaagc tgaagcggtt
     1861 ggatctctac ggcattcccg caaccgacat gagccctgtc gaaaacctca cggaactccg
     1921 gtacatctgg atctatgcga cgaagttcga agactactcg ccgctcgcca acctgacgaa
     1981 gctcgaggag ttccgcgccg gaatttcaaa gctcgatact cttgaatacc tggagagcat
     2041 gcccaagctg aagatcgtct ccatgctccg cgaggacatt caggactttt cgccgctggc
     2101 gaagtgcccg aacctgaccg aggtcaacct ggaagctgtg aaaggagtta tcgacctgag
     2161 cgttttcgcg gaacacaaga agctgaagaa tttgttcgtc cgcaatgcag aggtcaagaa
     2221 cgccgaagcc atcgtcgccc tgccgaggct ggagtggttg atcctcatcg acacgaaggg
     2281 tattgccgac gtctctattt tcaaggatct tcccaaactt aacagggtct ccgtgaagga
     2341 ggggcagttc ccggccgaac aggtcgaggc tttgggtaaa aaagcgtcgg tgcagaagtg
     2401 atgccgcgga aggaggccgc tactgcctcc ttcgacggcc ttcgaggggg attgatccgc
     2461 atgaagaggt ctcttgagga ggctcccgaa cagagtgtac cgcaaggtcg gcgcagtaaa
     2521 aaactctccc gtgccgaact tcgtatccgg ttgcggctcg aacccttcaa cctcacaaaa
     2581 aaagagacgg aaatagccgt gtttcttgtg gagggattgg agagaaacga gatattgttt
     2641 cgatgtgata ttaccaataa tacattaaaa acgcatattc gtcggatcta tcggaaactt
     2701 gaggtgagaa aaaggagcga aatcgcttca agactcgcgc tgacgtgaca aaacgatatt
     2761 ggaggagtac tcttatatgc accccggtat catccgggga aaactgtcgg agcggcgagc
//
LOCUS       DAOTIL010000301         2808 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminobacteriaceae bacterium isolate AMR_MDS_3026
            NODE_42454_length_2808_cov_4.547040, whole genome shotgun sequence.
ACCESSION   DAOTIL010000301 DAOTIL010000000
VERSION     DAOTIL010000301.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868050
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminobacteriaceae bacterium (wastewater metagenome)
  ORGANISM  Aminobacteriaceae bacterium
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae.
REFERENCE   1  (bases 1 to 2808)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2808)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:19:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,224
            CDSs (total)                      :: 3,198
            Genes (coding)                    :: 3,184
            CDSs (with protein)               :: 3,184
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2808
                     /organism="Aminobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_42454_length_2808_cov_4.547040"
                     /isolate="AMR_MDS_3026"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:3034609"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..522)
                     /locus_tag="P5201_07130"
     CDS             complement(<1..522)
                     /locus_tag="P5201_07130"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012792.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HRV98342.1"
                     /translation="MNATIVIRNEKEADYKRVEEITRKSFWNLYVPGCTEHYLVHVLR
                     THEDFLPELDFVIEVDGRIIGNIMYTKTLLVDESGEEKNILTFGPVCIVPEYQRKGYG
                     KRLMERSFERAVALGYDVIVIFGNPNNYVSRGFKSCKKFNVCLGNGTFPAAMMVKELK
                     PGVLDGRKWTYHQS"
     gene            complement(671..>2808)
                     /locus_tag="P5201_07135"
     CDS             complement(671..>2808)
                     /locus_tag="P5201_07135"
                     /inference="COORDINATES: protein motif:HMM:NF018000.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ion transporter"
                     /protein_id="HRV98343.1"
                     /translation="NKREYFADWWMDWIAAIPWDIFLFFLFPGGGASMLRLLRLPRIF
                     RLLRFRHLKHSDAARRLSYRFRRLMEVSIFRQILTMLLVSFVFVWIFTTVLDGLGVQS
                     EHGNNFWFSIITMISSDSIFEIHNQETLVKVLVLIFSFIGIVLFNGVLIAIIIGKLME
                     HLDDLKQGRGDVRERGHIILLGWNECIPHIVDELESYCRTERKRLIRVVVMREHPPES
                     PDQVVESRPHVEVISRTGSFQNAQALERISAHKASAVVVLGGRAADRKLSERLNDPIV
                     TRTLVALETLLDSKPNERHSPVIVLNYLDLSRSCHVTEFLRPFGNRSTKVFFNPLFFT
                     GKLIASMCINPYAEDIFNELLTPEGNEFHFVRLPPGEKMLWRDMMNAFPKSIPVGYRD
                     ASGELRMVPRPDEEVPESAEVVVLSENACDASIFAPPPPGSLPDARAPSPRCKNILPS
                     GMLVIVGVNPQLPFIVEETCSLGMTVHIVDNQTKEEFAAWYDEYSRTPLPESVVFREC
                     RFRSEEEICAAIALASADRIILLADGHLLDAATPDQIDAETISKLLMLGQLIEEQGRE
                     GIHLIVETLTLDSEVVVRNIRNCSNVIGPLTIGRLLTTFTLQPEFEALFRTIIQYGEI
                     DIACRPALDSVPGWKPGETTFADLLAGSRNSCIPLGWVKLPEEGAALEATMRRAAPKV
                     ELNPPKSSIVPERSEIVFLHRQGEKTLRP"
BASE COUNT          546 a          795 c          863 g          604 t
ORIGIN      
        1 gctttggtgg tacgtccact ttcttccgtc caggacgccc ggtttcagtt cttttaccat
       61 catcgccgcg gggaatgttc cgttcccgag gcagacgttg aactttttgc agcttttgaa
      121 accgcggctg acgtaattgt tggggttgcc gaaaatgacg atcacgtcgt acccgagcgc
      181 aaccgcgcgt tcaaaggagc gctccataag tctttttccg tacccttttc tctggtattc
      241 gggcacgatg cacaccgggc cgaaggtaag gatgtttttt tcttctccgg actcgtcgac
      301 gagcagtgtt ttcgtataca tgatattccc gatgattcga ccgtcgactt cgattacaaa
      361 atccagctcc ggcagaaagt cttcgtgggt ccgtaggaca tggactaaat agtgctccgt
      421 acaccccggt acgtataaat tccagaaaga tttcctggtg atctcttcga ctctcttata
      481 atcagcctct ttttcatttc tgatgacgat cgttgcgttc atagaaagcc tccactattt
      541 ttatgccccc gaaacagtag cgcaaggata ttcgatgaac atccttgcct ttccgggaga
      601 agttcgcttg cgggacgctg gaaggcctcg gcaagcgaca attttttatt atactcccgt
      661 tctgctcatg ctacgggcgg agcgttttct ctccctgcct gtgtaaaaaa acgatctccg
      721 atctctccgg aacaatcgag ctcttcggcg gattcagctc caccttggga gcggcgcggc
      781 gcatcgttgc ttccaaggca gccccctcct cggggagttt gacccacccc agcgggatgc
      841 agctgttgcg cgagcctgcg agcaaatcgg cgaaggtggt ctcgcccggt ttccagccgg
      901 ggacggagtc gagcgccggg cggcaggcga tgtcgatctc tccgtactgg atgatcgtgc
      961 ggaagagcgc ctcgaactcg ggctggaggg tgaatgtggt cagtaggcgg ccgatggtca
     1021 acgggccgat gacgttggag cagttcctga tgttgcgcac caccacctcg gaatccaggg
     1081 tgagcgtctc gacgatcagg tgaatgccct ccctcccctg ctcttcgatc agttggccga
     1141 gcatcagcag cttcgagatg gtctccgcgt cgatctggtc gggtgtggcg gcgtcgagca
     1201 gatggccgtc cgccagaagg atgatcctgt cggccgaggc cagggcgatg gcggcgcaga
     1261 tctcctcctc ggagcggaag cggcactcgc ggaagacgac gctctccggg agcggcgtcc
     1321 tggagtattc gtcgtaccac gccgcgaatt cttccttcgt ctggttatcg acgatatgca
     1381 ccgtcatgcc gagcgagcat gtctcctcga cgatgaacgg aagctgcgga ttgacgccga
     1441 cgatcacgag catccccgaa gggaggatat ttttgcagcg gggcgacgga gcgcgggcat
     1501 cggggaggct tccgggaggc ggaggtgcga agatcgacgc gtcgcaggcg ttttcgctga
     1561 gcacgacgac ctcggcggac tcgggaacct cttcgtcggg gcggggaacc atccgcagtt
     1621 cgcccgatgc atcccgatac cccacgggaa tcgacttcgg aaaggcgttc atcatgtcgc
     1681 gccagagcat cttctctccc gggggcaggc ggacgaagtg gaactcgttc ccctcgggtg
     1741 tgagcagttc gttgaagatg tcctccgcgt aggggttgat gcacatggag gcgatcagct
     1801 tgccggtgaa gaagagcggg ttgaagaaga ccttcgtgct tctgttgccg aacgggcgca
     1861 ggaactcggt cacatggcag cttctgctga ggtcgaggta gttcagcacg atcaccggag
     1921 aatgccgttc gttcggcttg ctgtcgagca gcgtttccaa agcgacgagc gtccgggtca
     1981 cgatggggtc gttcagccgc tccgagagct tcctgtcggc ggcccgtcct ccgaggacga
     2041 ctaccgccga cgccttgtgc gcggagatgc gttcgagcgc ctgcgcgttc tggaagctgc
     2101 ccgtccgcga gatcacctcg acgtgcggcc tcgactcgac gacctgatcg ggtgattccg
     2161 gcgggtgttc ccgcatcacc accactctga tcaggcgctt ccgttccgtt ctgcagtagg
     2221 actccagctc gtcgacgatg tgcgggatgc attcgttcca gccgagcagg atgatgtgcc
     2281 cccgttcgcg cacgtcgccg cgcccctgct tcaggtcgtc gaggtgttcc atgagcttgc
     2341 cgatgatgat ggcgatgagg acgccgttga agaggacgat cccgatgaac gagaaaataa
     2401 ggacgagcac cttgaccagc gtctcctgat tgtgaatctc gaagatgctg tccgagctga
     2461 tcatcgtgat gatggagaac cagaagttgt ttccgtgttc ggactgcacg cccaatccgt
     2521 ccagaacggt tgtaaatatc cagacgaaga cgaacgaaac gagaagcatc gtcagaatct
     2581 gccggaagat ggagacctcc atcaggcgcc ggaagcggta ggagaggcgg cgcgcggcgt
     2641 ccgagtgctt gaggtgacgg aatctgagca gccggaagat cctcgggagc cggagcaatc
     2701 ggagcatcga ggcgcctccc cccgggaaga ggaagaagag gaaaatatcc cacggaatgg
     2761 cggcgatcca gtccatccac cagtcggcga agtactctcg cttgttcg
//
LOCUS       DAOTIL010000302         2801 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminobacteriaceae bacterium isolate AMR_MDS_3026
            NODE_42603_length_2801_cov_3.464312, whole genome shotgun sequence.
ACCESSION   DAOTIL010000302 DAOTIL010000000
VERSION     DAOTIL010000302.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868050
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminobacteriaceae bacterium (wastewater metagenome)
  ORGANISM  Aminobacteriaceae bacterium
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae.
REFERENCE   1  (bases 1 to 2801)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2801)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:19:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,224
            CDSs (total)                      :: 3,198
            Genes (coding)                    :: 3,184
            CDSs (with protein)               :: 3,184
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2801
                     /organism="Aminobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_42603_length_2801_cov_3.464312"
                     /isolate="AMR_MDS_3026"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:3034609"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..794
                     /locus_tag="P5201_07140"
     CDS             <1..794
                     /locus_tag="P5201_07140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005662101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HRV98344.1"
                     /translation="AAPEAVSVVVPTSMHFEIARKALKNGIHVLIEKPVTTTVNEAEQ
                     LLRIAAESDLVLQVGHIERFNSAVQYISKIVHEPLFLQSRRLGPYSPRISDVGVVLDL
                     MIHDIDIILSLVRSEISDISATGRCIRSDHEDIASAQISFANGSMAHILVSRVSERRL
                     RQLEIMEPERYVTIDYETQDVSINRCVRQNNNSLVEVIEHPVFPKSEPLKLELQHFVT
                     CVREGKQPLVGITDGKRALEVAVSVLKQIHLDDQQFPEGRQAVSC"
     gene            993..1211
                     /locus_tag="P5201_07145"
     CDS             993..1211
                     /locus_tag="P5201_07145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014163211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis protein FliR"
                     /protein_id="HRV98345.1"
                     /translation="MPIINIQALIALALFLASLFIARIVVRIQNGSLPGGALWVLYLR
                     MLLGFLFAGAIVLAFYSFAGIDIISKHL"
     gene            1975..>2801
                     /locus_tag="P5201_07150"
     CDS             1975..>2801
                     /locus_tag="P5201_07150"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRV98346.1"
                     /translation="MTNSLLAVSSTTSREITLYSLPELRPSGRLEFPEGDRLFEPVSI
                     AVYGGWIAVADRNNSKVYVQSFLDAQRRFVIEADTPRDLEWSSLGDLFILHESGNVTK
                     CSISFSGESAPASETVLSDAREGWSLFSIHDRVFCIDISGSRLWELFPAPRGESLAML
                     SLFSPSISREQDKESFILEGTISGPFRTYMSLNSAVVTSVWNERLLGGAYVPSVSEHH
                     GMTLYFKPDDAARSRNGEVPAASGREVLGALMEAWHSRRGDIANIVVAASTPFSFEET
                     "
BASE COUNT          617 a          672 c          744 g          768 t
ORIGIN      
        1 gcgcggcgcc cgaggccgta agcgttgtcg tcccaaccag catgcacttt gagattgcac
       61 gaaaagcctt gaaaaacgga atccacgtgc ttatcgaaaa accggtgacc acgacggtca
      121 atgaggcgga gcagctgctc agaatcgccg ccgagtcgga tcttgtgctt caggtagggc
      181 atattgaacg gttcaacagc gctgttcagt atatctcgaa gatagttcac gaaccgctct
      241 tccttcaatc ccgccggctt ggtccctatt cccccagaat cagcgacgtc ggggttgttc
      301 ttgatctgat gatccatgat atagatatta tcctttcgct cgttcggtcc gaaatctccg
      361 atatttcggc gactggcaga tgcatacgat ccgatcatga ggatatcgcc tccgcgcaga
      421 tttcctttgc aaacggttca atggcgcata tcctggtaag ccgcgtctcc gaaagaaggc
      481 tgcgtcagct tgaaatcatg gagccggaac ggtatgtcac gatcgactac gaaacgcagg
      541 atgtttcgat caaccggtgc gttcgtcaga ataacaacag cctggtagag gtgatcgagc
      601 acccggtgtt tccgaagagc gaacccttga agcttgagtt gcagcacttt gtgacgtgtg
      661 ttcgcgaagg gaaacaacct cttgtaggca tcactgacgg gaaacgggct ttggaagtcg
      721 cggtttccgt cctgaaacag attcacttgg atgatcagca attccccgaa ggccgccaag
      781 ctgtttcatg ttgagtatcc tctctgaaat cgtgttatag tggtaccccg gaggtaatcg
      841 atccggggta ctttttttcg acagagcatg tgtttttttc ttttttttct cctcgcacga
      901 gaggtaaaat ctttatttga agtggcgtaa tggtttttta tagggtatcc taatcatgat
      961 taccctaatt cacacacgaa gcggagaatg ccatgcctat tatcaatatc caagcgctga
     1021 tcgcccttgc tctttttctt gcttcgctgt ttatagcgag aatcgttgtg cgtattcaaa
     1081 atggttcgtt gcctggcggg gctctgtggg ttctctacct gagaatgctt cttggttttc
     1141 tttttgccgg ggcgattgta cttgcttttt attcttttgc cgggatagat ataatttcaa
     1201 aacatctttg atgggcagtg gcttgggaag gggacgaaag agacgatgtt gtttttcaaa
     1261 cgcctgagga ggcatatcat tgtacttctt ctattttccg tccttttctc tggagcgccg
     1321 acggacggcg ccactcttcc cccagccgac agagaggaat cggagcgttt ttttgcgctg
     1381 gggtacgaag actttattaa aagggagtat gcttctgcgc ttgaaaatct agacagagca
     1441 ttgaagctga atacgtatct tgttgactac tatcttctgc gcggtctcgt ccttcatcgt
     1501 atcggacgaa ccgacgaggc tgtgaagtcg atacgttact acctggaagt tcgcccaagg
     1561 gacagcgctg cccccagaat tttggaacgc tatcgggatg agcaattttt catagaacag
     1621 ttcctttcag gggaaccttt acaatctgca gttgtctcct ccaggaaaga tgtcaaaacg
     1681 gctttttcgc tgggaactct gcaaattcca ggcgtaaacg gtctggggaa agcaacgtcg
     1741 tattcagacg gcgttttcct ttcggatacc tttgggaaca gagtgggttt tcgtctccct
     1801 ggagaaaagc ctttccagtt tgtcgagata ccctccccgg tggttgcgct tccaacagga
     1861 ggtaaaagct tttatgtagt atcagagaac ggggaggtct tttctcttcc ggaaggggaa
     1921 cgtagtccgg tacggatagg gaaaatttca acgggcctct cagacgcagc gttagtgaca
     1981 aacagcctgc ttgcggtttc cagcacgacg tccagagaaa tcaccctgta ctcgcttccg
     2041 gaactcagac cttccggacg gctcgagttt ccggaagggg atcggctttt cgaacccgtt
     2101 tccattgccg tatatggagg gtggattgca gtcgcagaca ggaataattc caaggtgtat
     2161 gttcagtcct ttcttgatgc gcaacgccgc tttgtgatag aggcggatac cccgcgggac
     2221 cttgaatggt cttcattggg agatcttttt attctccatg agtcgggaaa tgtgacgaaa
     2281 tgcagcatct ctttttcagg agagagcgcg ccggcatcgg agacggtact ttccgatgcg
     2341 cgcgaaggct ggagtctctt ttcgatccat gatcgggttt tttgtatcga tatttccggc
     2401 tcaagacttt gggagctgtt tccggcgcct cgcggagaga gcctcgcgat gctctctctt
     2461 ttctcccctt caatctcgcg ggagcaggac aaagagagtt ttattctcga aggaacaatc
     2521 agcgggccgt ttcgaacgta tatgtccctg aactccgctg ttgtgacttc cgtctggaat
     2581 gagcgccttc tcggcggagc gtacgttcct tctgtttcgg agcatcatgg aatgacgctg
     2641 tactttaaac ctgatgatgc agcccgatca cgtaacggcg aggttcccgc ggcttccggg
     2701 agagaagttc tgggcgcgtt gatggaagcg tggcattcca gaaggggcga catagccaat
     2761 attgtcgttg cggcgagcac ccctttttcg tttgaagaaa c
//
LOCUS       DAOTIL010000303         2784 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminobacteriaceae bacterium isolate AMR_MDS_3026
            NODE_42923_length_2784_cov_3.166361, whole genome shotgun sequence.
ACCESSION   DAOTIL010000303 DAOTIL010000000
VERSION     DAOTIL010000303.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868050
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminobacteriaceae bacterium (wastewater metagenome)
  ORGANISM  Aminobacteriaceae bacterium
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae.
REFERENCE   1  (bases 1 to 2784)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2784)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:19:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,224
            CDSs (total)                      :: 3,198
            Genes (coding)                    :: 3,184
            CDSs (with protein)               :: 3,184
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2784
                     /organism="Aminobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_42923_length_2784_cov_3.166361"
                     /isolate="AMR_MDS_3026"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:3034609"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..427
                     /locus_tag="P5201_07155"
     CDS             <1..427
                     /locus_tag="P5201_07155"
                     /inference="COORDINATES: protein motif:HMM:NF024700.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HRV98347.1"
                     /translation="LHSHSGAAEGWRSEESFSDGTLRLIGLLWSLQEGGPVLLLEEPE
                     LSLNEGIAERLPEVMERLRNGSSKRSQLVICTQSEALLSNPGIDGRGVIALEVGAQGS
                     RGRVLNAQEMEALDAGFSVAEAVLPKLRPEFLNRLGLKE"
     gene            424..1032
                     /locus_tag="P5201_07160"
     CDS             424..1032
                     /locus_tag="P5201_07160"
                     /inference="COORDINATES: protein motif:HMM:NF025468.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4276 family protein"
                     /protein_id="HRV98348.1"
                     /translation="MMYWNVATEDELSESVAERLLRSLPPPVELVNRFRRRGFGYLRK
                     NAGKWRSLARTQAVLLLTDLDRAACPPGLISEWFGEHTRMPDRLLVRVVVRETESWLL
                     ADHEAMRSLLDIHSGLPDAPDTLPDPKQTLLTLARQAPREVRLGLVRQEGTRLRQALR
                     YNSLLSEWVFTEWEPERAAKRSESLRRAIHRIKERALEFSGK"
     gene            1232..2113
                     /locus_tag="P5201_07165"
     CDS             1232..2113
                     /locus_tag="P5201_07165"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRV98349.1"
                     /translation="MSSGKSAVPARSFRGPRVRRGGSVVLWLLFLLLAALLPVPRNEA
                     RAQTEGGREARSPSNEALRERIALLEKALSFRGEGERMEQGGDFLGALLSFKRSHTLY
                     PDPALERKIRELTAGEPSSPRPDLQPLRDRIALLEELLAGLGTLDSLFGPEETPVSSG
                     PVVAVPPEEEFTGSENPDWKNYPDLPEEKAAIEESFDELRNALKEGDIERAVSVIDED
                     RREEYAALFAHKPEAMPSFAELLENAEMSFFSAAERADPATSTALRTSEYAVVLDGFT
                     FYVRWIKVDGKWVLFDF"
     gene            2145..>2784
                     /locus_tag="P5201_07170"
     CDS             2145..>2784
                     /locus_tag="P5201_07170"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRV98350.1"
                     /translation="MRIRRLPYIVVIIASILWTFAAPSFVEAWSQQPHSQINSEAITL
                     FVRRMAGKEKFRLGPFVDNALQQPLRGVAVTSSSLTASGFVSAEAEYSMRRWIVMGGD
                     WADEPHLYSSVRHFYDPLRITGVAYLTDQSWAHGHYDSPQIDARTWGLDHPDNPFTLF
                     TALAAYKAALEVRDDVPLPSSISAPHFKSTMNLVARDHEDQRKLHLSRAYRAL"
BASE COUNT          523 a          793 c          881 g          587 t
ORIGIN      
        1 gctgcattcg cactctgggg cggcggaagg gtggcggagc gaggagtcat tttccgacgg
       61 tacgcttcgt cttatcggcc tcctttggag tttgcaggag ggcggccccg tgttgttgct
      121 ggaggagccc gaactctcgc tgaacgaggg tattgcggaa aggctgccgg aagtgatgga
      181 acggcttcga aacgggagtt ccaagcgttc ccagctcgtg atctgcaccc agagtgaagc
      241 gttgctttcg aatccgggga tcgatggtcg cggagtgatc gcgttggaag tcggcgcaca
      301 gggttcaagg gggcgtgtgt tgaatgcgca ggagatggaa gcgctggacg cagggttttc
      361 ggttgcggag gcagtccttc ccaagttgcg ccctgaattc ctgaaccgcc tcggattgaa
      421 ggaatgatgt attggaacgt ggcaaccgag gatgaattga gcgaatccgt tgcggaacgt
      481 cttcttcggt cgctcccgcc gcctgtggag ctcgtcaaca gattccggag aagaggattc
      541 ggttatcttc ggaagaatgc cgggaagtgg cgaagtttgg cacggactca ggcggttctg
      601 ttgttgacgg acctggaccg ggccgcatgt cctccgggac ttatctccga atggttcgga
      661 gagcacacca gaatgcccga tcgccttctc gtacgcgtcg tcgtccggga gacggagagc
      721 tggctgcttg cggaccacga ggctatgcgc tctctgcttg atattcattc cggattgccc
      781 gatgccccgg atacgctgcc cgaccccaaa cagacgttgc tcacgcttgc acggcaggcg
      841 ccccgcgagg ttcgtctcgg tctggtgcgg caagagggaa ctcgtctgcg gcaggcgctt
      901 cgatacaaca gccttctttc ggaatgggtt tttaccgaat gggaacccga gcgcgcagcg
      961 aaacgttccg agagtttgcg gcgtgcgatt cacagaataa aggagcgcgc tcttgagttc
     1021 tccgggaagt gaaggttcta taaatatttg caagtaagtg aaaatccata ttttcttgta
     1081 gccttcatcg cagtcctccc tgtttgacat ttcaagcttg gtagaataag ctgtgcgtca
     1141 acaaagcgca gtatgaaatt gcgggcgcgg cctcttttta cagaaggaat cctgcctgca
     1201 ttccgtgtgt tctgaaggag gtgcggagat catgtcttcc ggaaaaagcg cagtaccggc
     1261 ccggtcgttt cgcgggccgc gcgtacgccg ggggggaagc gtcgtactgt ggttgctgtt
     1321 tcttcttctc gcggcgcttc ttcccgttcc ccgaaacgag gcgcgtgcac agacggaagg
     1381 gggacgcgaa gcgcgttctc cgtcgaacga agcgctccgc gagcgtatcg cgctgcttga
     1441 aaaggcgctc tcctttcgcg gcgagggcga gcggatggag caggggggcg actttctcgg
     1501 cgcgctgctt tcattcaaac gaagtcatac gctctacccc gatccggcat tggaacggaa
     1561 aatccgtgag ctgacggcgg gagagccttc ttcgccgcgc cctgatctgc aacctctgcg
     1621 ggacaggata gccctgctcg aggagctgct tgccggcctt ggtacgcttg attctctgtt
     1681 cggcccggag gagacgccgg tttcttccgg cccggttgtc gccgttcccc cggaagaaga
     1741 gtttaccgga agcgagaatc cggactggaa aaactacccg gatctgcccg aggagaaggc
     1801 ggcgatcgag gagtccttcg acgagttacg gaacgcgctg aaagagggcg atatcgaacg
     1861 ggccgtctcc gtgatcgacg aggaccgccg ggaggagtac gccgcgctct tcgcacacaa
     1921 gcccgaggcc atgccttcgt tcgctgaact cctcgaaaac gcggagatga gttttttcag
     1981 cgccgcggag cgcgccgatc cggcgacctc cacggcgctt cgcacatcgg agtacgctgt
     2041 cgtcctcgac ggttttacgt tctatgtccg gtggataaag gtcgacggca agtgggttct
     2101 cttcgacttt taacgtctcc ggccgggaaa ggagggaaag taccgtgcgt attcgcagac
     2161 tgccgtatat cgtagtcatc atcgcgtcca tcctttggac gttcgccgcg ccgtccttcg
     2221 tcgaagcgtg gagccagcag ccgcacagcc agatcaactc ggaggcgatc actcttttcg
     2281 tccgccggat ggcgggaaag gaaaagttcc ggctcggccc cttcgtcgac aatgcgctgc
     2341 agcagcccct gcgcggcgtt gcagtgacaa gttcttcgct gacggcctcc ggttttgtct
     2401 ccgcggaggc ggagtattca atgcgccgct ggatcgtcat gggaggcgac tgggccgacg
     2461 aaccgcatct ctactcgtct gtgcgccact tctacgaccc gctgcggata acgggcgtgg
     2521 cgtacctgac cgaccagagc tgggcgcatg gccactacga cagcccgcag atcgacgcta
     2581 gaacgtgggg gctggatcac ccggataatc cgttcacgct cttcactgcc cttgccgcgt
     2641 acaaggccgc gctggaagtc cgcgacgacg tcccgcttcc ttcgtcgatc tccgcgccgc
     2701 acttcaagtc gaccatgaac ctcgtcgcac gcgatcacga ggaccagagg aagctccatc
     2761 tctcaagggc gtaccgcgcc ctcg
//
LOCUS       DAOTIL010000304         2781 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminobacteriaceae bacterium isolate AMR_MDS_3026
            NODE_42969_length_2781_cov_3.908291, whole genome shotgun sequence.
ACCESSION   DAOTIL010000304 DAOTIL010000000
VERSION     DAOTIL010000304.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868050
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminobacteriaceae bacterium (wastewater metagenome)
  ORGANISM  Aminobacteriaceae bacterium
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae.
REFERENCE   1  (bases 1 to 2781)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2781)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:19:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,224
            CDSs (total)                      :: 3,198
            Genes (coding)                    :: 3,184
            CDSs (with protein)               :: 3,184
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2781
                     /organism="Aminobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_42969_length_2781_cov_3.908291"
                     /isolate="AMR_MDS_3026"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:3034609"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..888
                     /locus_tag="P5201_07175"
     CDS             <1..888
                     /locus_tag="P5201_07175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005659533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinylglutamate desuccinylase/aspartoacylase
                     family protein"
                     /protein_id="HRV98351.1"
                     /translation="HPTEPASMVTATLFLERAQVTKGSLIVIPQANLLGFTHNSPQEA
                     HPQSISFPTADGGKRTFRYGSRLTNTIYEWPSPDIYIHPASGQQLPGKERGNLNRCYP
                     GVPDGSITEQVAYALTELVRRERVDLAFDLHEASPEYPVVNAIVAHERSMELAAMTTM
                     DLEAAGIMMRLEPSPKNLRGLTHREWGNFTETMPVLMETGNPVQGRLRGRTDERLALT
                     GADKAYVKAAALGRLYIPYEGDQKIEYRVGRHATAIMSFLLNLDLLNEERGVLLENIP
                     TFEELQENGVGAYLAPLKL"
     gene            1049..>2781
                     /gene="ggt"
                     /locus_tag="P5201_07180"
     CDS             1049..>2781
                     /gene="ggt"
                     /locus_tag="P5201_07180"
                     /EC_number="2.3.2.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR00066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006750 - glutathione biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyltransferase"
                     /protein_id="HRV98352.1"
                     /translation="MRKSFVALFLMIFVLAGSAGAADFEVKEVLAGEGMVSSAHELAS
                     QAGVEILQKGGNAIDAAVATMLALNVVESNASGIGGGGFMTIRFAKTGEVVELDYREV
                     APNSATKDMYASEASKQAKESVLGGKAVGVPGIVMGIFTALEKYGTMSFSEVAEPALR
                     LAEEGFEVHPMQNGIITDEFEKLAKYSPECAFLPGGLPAEAGTVLKQPELAKAFRLLA
                     AEGPAAFYYGPIGEAVVAAVNKAGGDMSMKDLNDYKMIIQEPVMGTYRGYSIYSTPPA
                     SSGGTHIVQLLNIMENFPVAQWKHNSPEYLHYLAEAMKMVFADRQRYMADTAFVDVPL
                     AGLASKEYAKQLAEKITRYNVMQEVLPGDPWQFDGGKKTAYIGHDGNKHISTSSFSVV
                     DSDGNIVASTNTVNYFFGSGVIVPEYGFVLNNEMDDFAQDPASVNAPEPGKRPLSSMS
                     PTIVLDPEGRPFMSLGSAGAMRIITAMSQIIMNVVDFGMTMDEAIEQPRIFNVASSGK
                     AGKLLVEGGIDPATVMYLQFRGHNIDERARGGYFGTAQGILINYAKGMHQHGRINGGA
                     DSRRLGVPVGF"
BASE COUNT          604 a          795 c          811 g          571 t
ORIGIN      
        1 catccgacgg agcctgcctc catggtcacc gccacgctct tccttgagcg ggcgcaggtg
       61 acaaaaggaa gccttatcgt cattcctcag gcgaatctgc tggggtttac gcacaactct
      121 ccccaggaag cgcatccgca aagcatctct tttcccactg cggacggcgg caagagaacg
      181 ttccgatatg ggtctcgtct gaccaatacg atctacgaat ggccctcgcc ggacatttat
      241 attcacccgg cttcggggca gcagcttccg gggaaagaac ggggtaatct caaccggtgc
      301 tacccgggag ttccggacgg ctctatcacc gaacaggtcg cgtacgcgct cacggagctt
      361 gttcgcaggg agcgcgtcga tctcgcgttc gatctccatg aggcctcccc ggagtacccc
      421 gtcgtgaacg ccatcgtcgc gcatgagcgc agtatggagc ttgccgccat gacgacgatg
      481 gatctggaag ccgcgggtat tatgatgcgc cttgaaccct cgccgaagaa cctccggggg
      541 ctgacgcaca gggagtgggg aaatttcacg gagacgatgc ccgtgcttat ggaaacagga
      601 aatcccgtcc aaggccgcct tcgcggcaga acggacgaga ggctggcgct cacgggcgcg
      661 gacaaggcgt acgtgaaggc cgccgctctt ggccgcctct atatcccgta cgagggcgat
      721 caaaagatcg aataccgggt tggacggcat gcgacggcta ttatgtcttt cctcctcaat
      781 ctcgatctgc tcaacgaaga gagaggggtg ctcctggaga acatcccgac atttgaagag
      841 ctccaggaga acggggtcgg cgcgtatctt gctcctctga aattgtagtt gttgcggcgc
      901 gaatcctgct tttcgttttt tattatataa taggtaagaa tcaggacgat acacgagttc
      961 agaggtgtgt ttcgcttcaa aagttccact gaaagaaaca tgcctccgga gcacggcaat
     1021 aatatatctt ttcaggaggt gttgtattgt gagaaagagt tttgtcgcgt tgttccttat
     1081 gatttttgtt ttagcgggaa gcgccggtgc ggccgacttc gaagtgaagg aggttctcgc
     1141 cggggaggga atggtctcct ccgcgcatga actcgcttcg caggccggag tggagattct
     1201 gcagaagggc ggcaacgcca tcgacgccgc cgtggcgacg atgcttgcct tgaacgtggt
     1261 cgaatccaat gcctccggta tcggcggcgg cggatttatg accatccgct tcgccaagac
     1321 cggagaggtc gtcgaactcg actaccgcga ggtagctccg aactcggcaa caaaggacat
     1381 gtacgcttcc gaagcatcga agcaggcgaa agagtccgtc cttggcggaa aagcggtcgg
     1441 cgttcccgga atcgtcatgg gcattttcac cgcgctggaa aaatacggca ccatgtcgtt
     1501 ctcggaagtt gccgagcccg cgcttcgtct tgcagaggaa gggtttgagg tccatccgat
     1561 gcagaacggg atcatcaccg acgagttcga gaagctcgcg aagtacagcc ccgagtgcgc
     1621 gttccttccg ggcggccttc cggcggaagc gggaacggtt ctcaagcagc ctgagctggc
     1681 aaaagcattc cgtctgcttg ccgcggaagg ccccgccgct ttctattacg gccctatcgg
     1741 cgaagctgtt gtcgctgcgg tgaacaaggc cggcggagac atgtccatga aggacctgaa
     1801 cgattataag atgatcatcc aggaacctgt tatgggtacg taccgcggat actccatcta
     1861 ctccacgcct ccggcatcca gcggcggaac gcacatcgtc cagcttctca acattatgga
     1921 gaatttcccg gtagctcagt ggaagcacaa ctctcccgaa tatcttcact accttgccga
     1981 ggcgatgaag atggtcttcg cagaccgcca gagatacatg gcggacactg cgttcgtgga
     2041 cgttcctctt gcaggcctcg cgagcaagga gtacgcgaag cagctcgctg agaaaattac
     2101 ccgctacaac gtgatgcagg aagttcttcc cggagatccg tggcagttcg acggaggcaa
     2161 gaagaccgcc tacatcggcc acgacgggaa caagcacatc tccacgagtt ccttctccgt
     2221 tgtcgatagc gacgggaaca tcgtcgcctc cacgaatacc gtgaactact tcttcggttc
     2281 cggcgtgatc gtccctgagt acggtttcgt tctgaacaac gagatggacg atttcgctca
     2341 ggatccggcg agcgtcaacg cgcccgaacc cggcaagcgt cccctttctt cgatgtcgcc
     2401 gacgatcgtt ctcgaccccg agggcagacc cttcatgtcc ctcggttccg cgggagccat
     2461 gagaatcatc accgctatga gccagatcat catgaacgtt gtcgacttcg gaatgaccat
     2521 ggacgaggca atcgagcagc cgaggatttt caacgttgcg agtagtggca aagcgggcaa
     2581 actcctcgtt gaaggcggca tcgatccggc gacggttatg tacctccagt tccgcggcca
     2641 taacattgac gagcgcgctc gcggcggcta cttcggcacc gcgcagggca ttctcatcaa
     2701 ctacgccaag gggatgcacc agcacggcag gatcaacgga ggcgccgaca gcagacgtct
     2761 cggcgttccc gtcggtttct a
//
LOCUS       DAOTIL010000305         2777 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminobacteriaceae bacterium isolate AMR_MDS_3026
            NODE_43047_length_2777_cov_3.440485, whole genome shotgun sequence.
ACCESSION   DAOTIL010000305 DAOTIL010000000
VERSION     DAOTIL010000305.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868050
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminobacteriaceae bacterium (wastewater metagenome)
  ORGANISM  Aminobacteriaceae bacterium
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae.
REFERENCE   1  (bases 1 to 2777)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2777)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:19:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,224
            CDSs (total)                      :: 3,198
            Genes (coding)                    :: 3,184
            CDSs (with protein)               :: 3,184
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2777
                     /organism="Aminobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_43047_length_2777_cov_3.440485"
                     /isolate="AMR_MDS_3026"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:3034609"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..461
                     /locus_tag="P5201_07185"
     CDS             <1..461
                     /locus_tag="P5201_07185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008713405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="prolyl-tRNA synthetase associated
                     domain-containing protein"
                     /protein_id="HRV98353.1"
                     /translation="SYEMIEHEAAYTVEDMDRVGFDEKTTVCKNLFVRDQKKKATQWL
                     VVLLKDKRADLDSLAEQLGTKHLSFASADRLQKYLGLARGEVTPLGVLNDAAHEVIVV
                     FDKDLTGRERLGVHPNDNSATLLLSFGDIKRFVEQNGNTVRYVTLKAETA"
     gene            complement(582..1307)
                     /locus_tag="P5201_07190"
     CDS             complement(582..1307)
                     /locus_tag="P5201_07190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009201850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II aldolase/adducin family protein"
                     /protein_id="HRV98354.1"
                     /translation="MLLRRERELVVEHCRMLATHKLTKGTGGNISALDREKRLFAISP
                     SGMDYFAMQPEDVVVLDLDGKTVDGERRPSSEMDMHRLLYVDREDINAVVHAHSTYAT
                     TLACLHWSLPALHYLIGYAVSRGPTNTSEASKESCQSLSAHASSARGDDVRCTPYAPF
                     GSQELAEIARDGMKGRYAVLLGNHGLLAAGPNMQYAFNAAEEIEFVCELYWRAKSVGD
                     PVILSEEDMNVALEKFKTYGQRA"
     gene            complement(1341..>2777)
                     /locus_tag="P5201_07195"
     CDS             complement(1341..>2777)
                     /locus_tag="P5201_07195"
                     /inference="COORDINATES: protein motif:HMM:TIGR04564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SYNERG-CTERM sorting domain-containing protein"
                     /protein_id="HRV98355.1"
                     /translation="GKNPEQEPFNVRFELYRDGVLAPLRTVTSTVDDGTGLTPLNAIK
                     TDYPHGWTPGTALDILASPPPDLVYNPALPVSFYDAAIKAVVTRHYAAALIQGGHTKT
                     FDSNYAAIYTRDLEEGAYTLKATAVGAAGAELATVSVPLTFGSVPDKIISRFSPENHM
                     ENVTAFAQKNNSHIYNDLFPGYWDASSLPHGGIPGTLFYEIVRRWRPNDLLEYMNGTI
                     RAVVYNVHVSSATQSVELGGLAYAGRLGSNSILWYHYDSGEVSLAYDLGNGQAASKLG
                     TIVPFPDGKRLVLVRAEIRGDGQTEPPTSDYVYTPELADKTVDWNVADGVAVKPKQLL
                     SLFGVVKPIQPALNDVVANDDGTYTINNRIATVRYTLLDGTQEMLTFDAKRVELTRNG
                     INNRPSIYEFRHDIPVPATFNKPWTVRVAAFDIYGTLVPGTEMSFTLAPYSPGGGGGG
                     GGCSVGAAPFALLLLLPLLVLAGKRGRT"
BASE COUNT          511 a          860 c          862 g          544 t
ORIGIN      
        1 tatcctacga gatgatcgag cacgaggcgg cctacaccgt cgaagacatg gaccgcgtgg
       61 ggttcgacga gaagacgacc gtctgcaaaa acctcttcgt ccgcgaccag aagaagaagg
      121 cgacccagtg gctcgtcgtg ctgctcaagg acaagcgggc cgacctggac agccttgcgg
      181 aacagctggg gacgaaacac ctcagcttcg cctccgcgga ccgcctccaa aaatacctcg
      241 gcctcgcccg gggcgaggtc acgcccctcg gcgtgctcaa cgacgcagcc cacgaggtca
      301 tcgtcgtctt cgacaaggac ctcacaggca gggaacgtct gggcgtgcac ccgaacgaca
      361 acagcgcgac gctgctcctg tcgttcgggg acatcaagcg gttcgtcgag cagaacggaa
      421 atactgtgcg gtacgttacg ttgaaagcgg agacggcgta gcgcgcaacg aaaaaccccg
      481 cttcggaacc tgcccgccgc attcctccgt tctccggaac aaacaaaccg ggagcccgaa
      541 attcagggct cccggttttc atgcgtattt ttccgaacgg ttcaggcccg ctggccgtag
      601 gtcttgaact tctccagcgc gacattcata tcctcttcgc tcagtatgac ggggtcgccc
      661 acgctcttcg cgcgccagta gagctcgcag acgaattcga tctcctcggc ggcgttgaag
      721 gcgtactgca tgttcggccc ggcggcgagg aggccgtgat tgcccagaag gacggcgtac
      781 cgccccttca tcccgtcgcg cgcgatctcc gccagctcct gcgatccgaa cggcgcgtag
      841 ggggtgcacc ggacgtcgtc tcctctagcg ctagatgcat gcgcactaag cgactgacac
      901 gactccttac tcgcttcgct cgtgttcgtc ggtcctctgc ttacagcgta tccgatcagg
      961 tagtggagcg ccggaaggct ccagtggagg caggcgagcg tcgttgcgta ggtggagtgc
     1021 gcgtggacga cggcgttgat gtcctccctg tccacgtaga gcagccggtg catatccatc
     1081 tcgctcgacg gccttctctc cccgtcgacg gtcttcccgt cgaggtcgag gacgacgacg
     1141 tcctccggct gcatcgcgaa atagtccatc ccgctcgggc tgatcgcgaa gagcctcttc
     1201 tccctgtcca gcgcgctgat gttgccgccg gtccccttgg tcagcttatg ggtcgccaac
     1261 atcctgcagt gttccacaac aagttcacgc tcacgccgga gcagcattcg tcgttcctcc
     1321 attgcaatac aagcgtaggc ctaggtcctt ccgcgctttc ccgcaagaac aagcaacggc
     1381 agaagcagca gcagcgcgaa cggcgcggcg ccgaccgagc atccgccgcc cccgccgccg
     1441 cctccgggcg agtagggagc gagcgtgaag ctcatctccg tacccggcac gagcgttccg
     1501 tagatgtcga acgccgcgac gcggaccgtc cacggtttgt tgaaggtcgc cggaacagga
     1561 atgtcatgcc gaaattcgta gatggacggt cggttgttga ttccgttgcg ggtcagctcg
     1621 acgcgtttgg cgtcaaatgt aagcatctcc tgcgttccgt cgagcagggt gtagcggact
     1681 gtcgcgatcc tgttgttgat ggtgtaggtt ccgtcgtcgt tggcaaccac gtcgttaagg
     1741 gcgggttgaa tgggcttcac cacgccgaaa aggctgagaa gctgcttcgg cttcaccgcg
     1801 acgccgtcgg ccacgttcca gtccaccgtc ttgtcggcaa gctccggagt atagacatag
     1861 tccgacgtcg gaggttcggt ctgtccgtcg ccgcgtatct ccgcgcggac taggacaagg
     1921 cgcttgccat ccgggaaggg gacgatggtt cccagtttgg acgccgcctg accgttcccg
     1981 agatcgtagg cgagcgacac ctccccgctg tcgtagtgat accagaggat cgaattggaa
     2041 cctagcctgc ccgcatacgc caatccgccg agttcgaccg attgggtcgc tgaagagacg
     2101 tggacgttgt acacgacggc cctgattgtg ccgttcatgt actccagcag gtcgttcgga
     2161 cgccagcgcc gcacgatctc gtagaagagc gtccccggta tgcctccgtg gggcagagag
     2221 gatgcgtccc agtatccggg gaagagatcg ttgtagatgt gcgagttgtt cttttgcgcg
     2281 aaggccgtta cattttccat gtggttctcg ggagaaaagc gggagatgat cttgtccggc
     2341 acgctcccga aggtcagagg tacgctgaca gtggccaact ctgcgcccgc tgcgccgacg
     2401 gccgtcgcct tgagggtgta ggctccctcc tccagatcgc gagtgtatat ggccgcgtag
     2461 ttgctgtcga aggtcttcgt atggcctccc tggatgaggg cggcggcgta gtggcgcgtc
     2521 accacggcct tgatcgccgc atcgtagaaa gaaacaggca gcgccggatt atagacgagg
     2581 tcgggaggcg gcgaagcgag gatgtccagc gcggttcccg gagtccatcc gtgcggatag
     2641 tccgtcttga tagcgtttaa cggcgtcaac cccgtaccgt cgtccaccgt gctcgtcact
     2701 gtgcgcagcg gggcgagcac cccgtcgcgg tacagctcga atcggacgtt gaagggctcc
     2761 tgctcgggat ttttgcc
//
LOCUS       DAOTIL010000306         2776 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminobacteriaceae bacterium isolate AMR_MDS_3026
            NODE_43078_length_2776_cov_3.016171, whole genome shotgun sequence.
ACCESSION   DAOTIL010000306 DAOTIL010000000
VERSION     DAOTIL010000306.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868050
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminobacteriaceae bacterium (wastewater metagenome)
  ORGANISM  Aminobacteriaceae bacterium
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae.
REFERENCE   1  (bases 1 to 2776)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2776)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:19:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,224
            CDSs (total)                      :: 3,198
            Genes (coding)                    :: 3,184
            CDSs (with protein)               :: 3,184
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2776
                     /organism="Aminobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_43078_length_2776_cov_3.016171"
                     /isolate="AMR_MDS_3026"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:3034609"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..456)
                     /locus_tag="P5201_07200"
     CDS             complement(<1..456)
                     /locus_tag="P5201_07200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015555873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008184 - glycogen phosphorylase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen/starch/alpha-glucan phosphorylase"
                     /protein_id="HRV98356.1"
                     /translation="MMSLHHQRELGSSIRKLMETDPAFRPVAYLSMEIGIKESLPTYS
                     GGLGILAGDILKSAADLGVPMVALTLLYRKGYFQQSFNEDGWQTEKPVLWQPVQELTL
                     LPNQVSLTLQNREVHVRVWQYEIVGNTGHPLPVYFLDTDFDNNHPDDRKL"
     gene            complement(643..2163)
                     /gene="malQ"
                     /locus_tag="P5201_07205"
     CDS             complement(643..2163)
                     /gene="malQ"
                     /locus_tag="P5201_07205"
                     /EC_number="2.4.1.25"
                     /inference="COORDINATES: protein motif:HMM:TIGR00217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004134 - 4-alpha-glucanotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0005980 - glycogen catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-alpha-glucanotransferase"
                     /protein_id="HRV98357.1"
                     /translation="MYNKEQKTEVFFLTRSCGILLPVSSLPGRWGIGDLGPGARSFVD
                     FLSRSGQKTWQILPLSETDGALGNSPYSSPSAFAGNHLFISPDDLFKCGLLTAADTAE
                     LPPFPGEKVDYPTVRAVKEKLLARAFERFAPSEEYETFLQQNEHWVFDYALFSALKQR
                     FGGLSWNQWPDELKLRDGNALRTAATELRESVDFLLFKQYLFFSSMERLRRESDEKGV
                     ELLGDLPIYVNFDSADVWAHPNLFHLDENLLPVEVAGVPPDYFSETGQLWGNPLYNWE
                     ALREDDFSWWIKRLQHSLSMFHLVRIDHFRGLLAYWAVPFGDRTAEKGVWRDVPSEAF
                     FASLRKNIPSASFLAENLGVITPDVTDAMERMSFPGMAVVLFAFGGGMKDNPHIPHNY
                     SATLAAYSGTHDNNTARGWYRDDASEEERNTLGRYLGRMPDEKEVPDIIVRMVLSSVA
                     QRAIIPLQDYLGLGSEARMNTPATPSGNWEWRADELCFTEELSIRIKQLSEIYGRN"
     gene            2224..>2776
                     /locus_tag="P5201_07210"
     CDS             2224..>2776
                     /locus_tag="P5201_07210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015556797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase"
                     /protein_id="HRV98358.1"
                     /translation="MPCSICLHGHFYQPPRENPWLEEVEIQESAAPWHDCNERISEEC
                     YGPNSAARIFDDAGWIRRIVNNYSRISFNFGPTLLSWMERKRPQYYQAVLEADRLGSA
                     RFSGHGPAIAQAYNHMILPLANRRDKITQIVWGIEDFQKRFQRAPEGMWLPECAVDSE
                     TLDILAEHGIRFVILAPRQAQAVR"
BASE COUNT          599 a          750 c          807 g          620 t
ORIGIN      
        1 gagtttgcgg tcgtcggggt gattgttgtc gaaatcggtg tccaggaagt agaccgggag
       61 cggatgtccc gtgttgccga cgatctcgta ctgccatacc cgtacgtgga cctcgcggtt
      121 ttgcagcgtg agcgacacct ggttgggcag aagcgtcaac tcctgcaccg gctgccagag
      181 cacgggtttt tccgtctgcc agccgtcctc gttgaacgac tgctggaaat accctttcct
      241 gtaaagaagc gtgagggcga ccatgggaac gccaaggtcg gccgcgcttt tcaggatgtc
      301 tccggcaagg atgcccagac ctcccgaata ggtcgggaga gattctttta tgccgatctc
      361 catggaaaga tatgctaccg gacgaaacgc cgggtcggtt tccattagtt tgcggattga
      421 actgccaagc tctctctgat ggtgcagtga catcatggga gcgactcctc ctctcaagta
      481 gtacagattc aaacgttggg gctttcgtgt tgcgaccgga gaagaaacgc tctggattta
      541 tcttgcgtat catccgccgg aagccgtgcc cgaaaaagga acccacgaag cctttgattc
      601 ataatagtac cagaaaaagc cctcttcttt gaagggggaa catcaatttc ttccgtaaat
      661 ttcggagagt tgctttattc tgattgaaag ttcttcggtg aagcacaatt cgtcagcgcg
      721 ccactcccag ttccccgagg gggtcgcggg cgtgttcatg cgcgcttcgc tcccaagtcc
      781 caggtagtcc tggagcggga tgattgcgcg ctgtgcaacc gaggagagta ccatccttac
      841 gataatgtcc ggaacctcct tctcgtccgg cattcggccg agatagcgcc ccagcgtgtt
      901 ccgttcttcc tccgaggcgt cgtcgcggta ccagccgcgc gcggtgttgt tgtcgtgtgt
      961 tccgctgtag gctgcaagcg ttgcggagta gttgtgcgga atgtgcggat tgtccttcat
     1021 cccgcctccg aacgcaaaaa gaacgactgc cattccggga aagctcattc gttccatggc
     1081 gtccgtgaca tcgggtgtaa tgacgcccag gttttcggcc aggaatgaag cggaggggat
     1141 gttctttcgg agtgaagcaa agaacgcttc cgacggcaca tctctccaga cgcctttctc
     1201 agcggtcctg tcgccgaaag ggactgccca gtaggcaagg agtccgcgga aatggtcgat
     1261 acggacgagg tggaacatgg agagcgagtg ctgcaagcgc tttatccacc atgaaaagtc
     1321 gtcttcccgc aaagcttccc agttgtacag cgggttgccc cagagttggc ccgtttcgct
     1381 gaagtagtcg gggggaactc ccgcaacctc caccggcagc agattttcgt cgaggtgaaa
     1441 aagattggga tgcgcccata cgtccgcgct gtcgaagttg acgtagatcg gcagatctcc
     1501 cagcagttcg acgccctttt catcgctctc tctgcgcaga cgctccatag acgagaagaa
     1561 gagatactgc ttgaagagca agaagtccac tgattcgcgc agctctgttg cggcggttcg
     1621 aagagcgttc ccgtcccgga gtttcagctc gtcgggccac tgattccacg aaagaccgcc
     1681 gaagcgttgt ttcaatgcgg aaaagagcgc atagtcgaag acccaatgct cgttctgctg
     1741 tagaaaggtt tcgtactcct ctgagggagc gaacctttcg aacgctcgcg ctaaaagttt
     1801 ctccttcacc gcacggactg tcggatagtc gaccttctct cccgggaacg gaggcagttc
     1861 cgccgtatcg gcggcagtga gcagtccgca tttaaaaagg tcgtccggtg agatgaaaag
     1921 atgatttccg gcaaacgcgg aaggcgagct gtacggagag ttgccgagcg ctccgtcggt
     1981 ttcggagagg gggagtatct gccatgtttt ttggcccgaa cgcgagagga agtcaacaaa
     2041 cgatctggct cccggaccaa ggtcgccgat gccccacctt cctggaagag aggaaacggg
     2101 aaggagaata ccgcaagatc tcgtcagaaa aaacacctcc gttttttgct ccttattgta
     2161 caccaagcga aaagagaggt gaagaaaggg tataatttat cccggaagaa aggagggttt
     2221 ttcttgccct gttcgatctg tttgcatggt cacttttacc agccgcccag ggagaacccc
     2281 tggctagaag aggtggaaat tcaagaatcc gcagctccct ggcatgactg taatgaacgt
     2341 atttccgagg agtgctacgg gccgaactcc gccgcgcgta tttttgacga cgcggggtgg
     2401 atccggcgta tcgtcaataa ctattcgcgg atcagtttca atttcggacc gacgcttctt
     2461 tcatggatgg agagaaaacg cccccagtac tatcaggctg tcctcgaggc ggaccgcttg
     2521 ggatcggcac gtttttcggg acacggtccc gccatagccc aggcgtataa ccatatgatc
     2581 cttcctcttg cgaaccgccg cgacaagatc acacagatcg tctgggggat cgaggacttc
     2641 cagaagcgct tccagcgggc gccagagggg atgtggctgc ccgagtgcgc cgtcgattcc
     2701 gagacgcttg atattctcgc cgagcatggc atacgcttcg tcatcctcgc cccccgccag
     2761 gcgcaggctg ttcggg
//
LOCUS       DAOTIL010000307         2776 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminobacteriaceae bacterium isolate AMR_MDS_3026
            NODE_43080_length_2776_cov_2.876148, whole genome shotgun sequence.
ACCESSION   DAOTIL010000307 DAOTIL010000000
VERSION     DAOTIL010000307.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868050
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminobacteriaceae bacterium (wastewater metagenome)
  ORGANISM  Aminobacteriaceae bacterium
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae.
REFERENCE   1  (bases 1 to 2776)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2776)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:19:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,224
            CDSs (total)                      :: 3,198
            Genes (coding)                    :: 3,184
            CDSs (with protein)               :: 3,184
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2776
                     /organism="Aminobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_43080_length_2776_cov_2.876148"
                     /isolate="AMR_MDS_3026"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:3034609"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..637
                     /locus_tag="P5201_07215"
     CDS             <1..637
                     /locus_tag="P5201_07215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013048820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DnaJ C-terminal domain-containing protein"
                     /protein_id="HRV98359.1"
                     /translation="CGGRGQVESRQQTPFGQFVSVNACPRCNGTGKIIKEKCPECGGQ
                     GRVRTQHKVEVKIPAGVDTGTRLRISGEGEPGVNGGTSGDLYLVVSVQEHPDFKREGG
                     DLHTRLSISFPQAALGCTVDVPVLSGEPETLDIPAGTQPGKVFTLRGRGVPRLRGPRG
                     KGDLFVHVTVDVPTKLTEKQRALLEELAREMNVTVEDSGVFGKFFRNLFEG"
     gene            775..1662
                     /locus_tag="P5201_07220"
     CDS             775..1662
                     /locus_tag="P5201_07220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013048819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11 methyltransferase"
                     /protein_id="HRV98360.1"
                     /translation="MNSSAESFWWYVTLEGRPGSEEVLASLADMSGSIGSEETAGEAS
                     VLLRAYFRSSQDLGFWLERLGGALSPWPEVRIADMGKIENKQWHTDWKEAFPPLAVGE
                     GLVVLAPWHKGNEPEGRMPLYIYPGSAFGTGYHESTQIVLTLLERTVVPGITAADIGA
                     GSGILSIAAVKLGAGHVRARDLDPAVLSEISHNLGLNEIPSGTVDLEEGNLLDGMDET
                     VDLLMANIIIEPLLEMLPNVPARLKEKGRALFSGLLVKERERFCAALEATGLRVIDEL
                     SLGDWWGVTVEPYAAPSRR"
     gene            1640..>2776
                     /locus_tag="P5201_07225"
     CDS             1640..>2776
                     /locus_tag="P5201_07225"
                     /EC_number="2.8.4.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008172 - S-methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016782 - transferase activity,
                     transferring sulphur-containing groups [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MiaB/RimO family radical SAM
                     methylthiotransferase"
                     /protein_id="HRV98361.1"
                     /translation="MLHHLAGKRIWICTLGCRSNQYEGEALANAFTEAEAILSDTPDC
                     DAAVLVSCTVTSVADKKCRQMIGRVRRTSPGAIIVACGCWAQHLPEEEAQSLGIHLLV
                     GNRKKQHVPFLLAQLFGDNSRRFSVCREDVLHSTEWDSLFLAKPLLHTRAFVKVQDGC
                     NHFCTYCAIPYARGFPVSRSPDDVLKEIRSIVSSDCREVVLTGVHLGLYGEYGEISLA
                     ELVRKVSLVEGLRRLRFGSIEPLGIDDGLLETLAETHAFQPHLHIPLQSGSDRVLSLM
                     NRGYSTAEYRDIVQRVRAFLGDDVHVSTDLLVGFPGEDERAFAETLAFLEECRFGKVH
                     VFPFSPREGTKAFSLPGRVPPQELSSRSKRALETGEKLLLEYSAR"
BASE COUNT          569 a          737 c          845 g          625 t
ORIGIN      
        1 gtgcggcggg cgaggacagg tggagagcag gcagcagacg cctttcggcc agtttgtctc
       61 ggtgaatgcc tgtccccggt gcaacggcac gggaaagatt attaaggaga agtgtccgga
      121 gtgcggaggc cagggaaggg tgcgcacgca gcacaaggtc gaggtgaaaa ttccggcggg
      181 cgtggatacc ggaaccaggc tacgaatatc cggagagggc gagccggggg taaacggagg
      241 aacctcgggc gatctctatc tcgtcgtgag cgttcaggag caccccgatt tcaagaggga
      301 gggcggagac ctgcataccc gtctttccat atctttccct caggccgctc tggggtgtac
      361 agttgatgta cccgtgcttt ccggcgagcc tgagacgctg gacatccctg ccggaacaca
      421 gccgggaaag gtattcactc tccgcggcag gggcgttccg cgccttcgcg gtcccagagg
      481 caaaggagat ctgtttgtcc atgtaacggt ggatgtgccg acgaagctga cggagaagca
      541 gcgcgctctt cttgaggagc tggccaggga gatgaatgtc acggtggagg attccggggt
      601 tttcgggaaa tttttcagga atctcttcga agggtaagtt ttttttcgag acgctcgttc
      661 agtaaaagag tattcgccct gtaaaaacgg cgcttcttct gctacactct ttatcagcag
      721 gaaaagggag gctgcactag cctcccttat ttcatcttcc gggaggaatt cggcatgaac
      781 agttcagcag aaagcttctg gtggtatgtc actctggaag ggcggcccgg gagcgaggag
      841 gttctggcct ctcttgcaga tatgtcggga agcattggtt cagaggagac tgccggagaa
      901 gcttccgtcc tcctgcgggc atactttcgc agctcccagg atctgggttt ttggctcgaa
      961 cgccttggcg gggcgctgtc cccgtggccg gaagtcagga tcgccgatat ggggaaaata
     1021 gagaacaaac agtggcatac cgactggaag gaagccttcc ctcctcttgc agtaggagaa
     1081 gggctcgtcg ttctggctcc ctggcacaaa gggaacgaac ccgaaggccg gatgccctta
     1141 tatatctatc ccgggtccgc gtttggcacg gggtaccacg agagcacgca aattgtgctg
     1201 acgcttctgg aacgtactgt cgtccccgga atcactgccg ccgacatcgg agcaggttcc
     1261 ggcatcctct ccattgccgc ggtcaagctg ggagccggac atgtcagggc gcgggacctc
     1321 gatcccgctg tgctctccga aatcagtcat aatctgggac tgaacgaaat cccctccgga
     1381 acggtggacc ttgaggaggg gaatcttctc gacggaatgg acgaaaccgt cgacctcctc
     1441 atggcgaata ttattatcga accgctcctc gaaatgcttc cgaatgtccc cgcacgactg
     1501 aaggagaagg ggcgcgctct tttttcaggt ttgcttgtaa aggagcgtga gcgtttctgc
     1561 gctgcgcttg aggcaacagg attgcgcgtt atcgacgaac tctcacttgg cgactggtgg
     1621 ggggtaacgg tagaacccta tgctgcacca tctcgccggt aagagaatct ggatctgcac
     1681 gcttggatgc cggtccaacc aatacgaggg cgaggctctt gccaatgcct tcaccgaggc
     1741 ggaagcaatt ctgagcgaca cccccgactg cgatgcggcg gttctggtga gctgtaccgt
     1801 cacttcggtt gcggataaaa aatgccggca gatgatcggg cgggtacgcc gaacgtcccc
     1861 cggcgcgatt atcgtcgcgt gcgggtgctg ggcgcagcat cttccggagg aagaggcgca
     1921 gtcgctcggg attcatctgc tcgtcggcaa ccggaaaaaa cagcatgttc cgtttcttct
     1981 tgcacaactc ttcggggata attcccggcg tttttcggta tgtcgggagg atgtccttca
     2041 cagtacggag tgggattctc ttttcctcgc gaagccgctg cttcataccc gcgccttcgt
     2101 caaggtgcag gacggctgca atcatttctg tacctactgt gcaattccct atgctcgagg
     2161 atttcccgtc agtcgctctc cggacgatgt actgaaggag attcgttcca tagtttcttc
     2221 cgactgccgt gaggtcgtgc tcaccggggt gcatctgggg ctgtacggcg agtacggtga
     2281 gatatctttg gcggagcttg tcagaaaagt atctttggtc gaggggcttc gccgtcttcg
     2341 cttcggttcg atcgaacctc tgggaatcga cgacggcctg cttgaaacgc ttgcagaaac
     2401 tcacgctttc caaccgcatc tccatattcc ccttcagagc ggaagcgacc gggtactctc
     2461 tcttatgaac agagggtatt cgacggcgga gtatcgtgat atagtgcaaa gagttcgtgc
     2521 ttttctcggg gatgatgtcc acgtcagtac cgatttgctc gtcggcttcc ccggggagga
     2581 cgaacgcgca ttcgcagaga ctcttgcttt ccttgaagag tgccgcttcg ggaaggttca
     2641 tgtcttcccg ttctcccccc gtgaagggac gaaagccttc tctctccctg gaagagtgcc
     2701 tcctcaagag ctctcctcaa gaagcaagag ggctcttgaa acaggagaaa aattgttgct
     2761 cgaatacagt gcccgc
//
LOCUS       DAOTIL010000308         2772 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminobacteriaceae bacterium isolate AMR_MDS_3026
            NODE_43182_length_2772_cov_3.070666, whole genome shotgun sequence.
ACCESSION   DAOTIL010000308 DAOTIL010000000
VERSION     DAOTIL010000308.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868050
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminobacteriaceae bacterium (wastewater metagenome)
  ORGANISM  Aminobacteriaceae bacterium
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae.
REFERENCE   1  (bases 1 to 2772)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2772)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:19:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,224
            CDSs (total)                      :: 3,198
            Genes (coding)                    :: 3,184
            CDSs (with protein)               :: 3,184
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2772
                     /organism="Aminobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_43182_length_2772_cov_3.070666"
                     /isolate="AMR_MDS_3026"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:3034609"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1262)
                     /locus_tag="P5201_07230"
     CDS             complement(<1..1262)
                     /locus_tag="P5201_07230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009165828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding protein"
                     /protein_id="HRV98362.1"
                     /translation="MYDVIIVGSGPAGIFAAMECVRHEKKVLIIDKGRLIRERKCPIV
                     EGTSKTCLNCSSCSIVSGWGGAGSASDGKLTLTTGFGGNLEESIGEEALLDMIAHVDS
                     VFVEYGADNHAYEPSGDIVRDTIRKASSVGIKILPARIRHIGTDASREVLNNMYEALK
                     PKCDILMGTFVEDLLIENGRACGVVLADGTVHRSASVIVAPGREGASWLAGVVAKLNI
                     PIASMPVDIGVRVEVPDSVCEDLTRHFYEIKCLYNTPTFDDRCRTFCMNPSGFVVYEY
                     NQAHDLVTVNGHSLKNVKSRNTNFAILVTKNFTQPFNDPIGYATHVAKLANMLAGGGI
                     LVQRLGDLRDGRRSTQSRIDRGMITPTANAQPGDLSLVLPHRYLTDVVEFLDGLNSIM
                     PGVNQNDTLLYGVEIKLYSLRLELRKNL"
     gene            complement(1359..1721)
                     /locus_tag="P5201_07235"
     CDS             complement(1359..1721)
                     /locus_tag="P5201_07235"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRV98363.1"
                     /translation="MALRVCEYCGKAWFDEGGEKQVALCPPCAQEVQGIYMLAWGYLR
                     DNTAEAGRHRKISIQQLADELGIDTKAINLLVKMGKIQVESFAGEGNADEIARNLEIL
                     RKLRKNVRGKSKYDRSGR"
     gene            complement(1726..2379)
                     /locus_tag="P5201_07240"
     CDS             complement(1726..2379)
                     /locus_tag="P5201_07240"
                     /inference="COORDINATES: protein motif:HMM:NF013843.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxynucleoside kinase"
                     /protein_id="HRV98364.1"
                     /translation="MGRVQIIVEGMTASGKSTVVNLLSERLGLRVMPEEFRDQYDLLR
                     RFHLERRWAFPMQLNFLVTRFAQYLCASESDEYILDRSVFGDKIYATLYYKLGYFRDS
                     QFGTYLMLYDSLLKHLAMPKLIIVLSCPFEEIMRRIRMRGRSDEIEAGEEYWRSLYDA
                     YASFLDFVRSEIGLSATRCITLDLGDPSFIHTPERVERFIEEVTEALDMEAPVTTGG"
     gene            complement(2441..>2772)
                     /locus_tag="P5201_07245"
     CDS             complement(2441..>2772)
                     /locus_tag="P5201_07245"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HRV98365.1"
                     /translation="EAGDRVLKDFCAAVKSRIRPTDFIGRWGGDEFAVLTPMSGRIAF
                     QIAEAIRDMIYNYRFLPDRILSSSMGVAEFRRSMDMNEFVKRAEDALAEAKKAGGNKA
                     ILAPFVP"
BASE COUNT          546 a          862 c          761 g          603 t
ORIGIN      
        1 tccaggttct tccgaagctc cagccggagc gagtacagct tgatctccac gccgtagagc
       61 agcgtgtcgt tctggttcac gccgggcatg atggagttga gaccgtcgag aaattccacg
      121 acgtcggtca gatagcggtg cggcagcaca aggctgaggt cgccgggctg cgcgttcgcg
      181 gtcggggtga tcatcccccg gtcgatgcgc gactgggtgg aacgccgtcc gtcccggaga
      241 tcgccgagcc gctggacgag aattccgcct ccggcaagca tgttcgctag ttttgcgacg
      301 tgcgtggcgt accctatagg gtcgttgaac ggctgcgtga agttcttcgt caccaaaatg
      361 gcgaagttcg tgttcctgga cttcacgttc ttcaggctgt gcccgttcac ggtgacgaga
      421 tcgtgcgcct ggttgtattc gtagacgacg aatcccgacg gattcatgca gaaggtgcgg
      481 cagcggtcgt cgaaggtcgg cgtgttgtac agacacttga tctcgtagaa gtgccgtgtg
      541 aggtcttcgc agacgctgtc cggaacttcc acccgcacgc cgatatccac cggcatagag
      601 gcgatgggta tatttagctt cgcgacaacg cccgccagcc aggaagcccc ctcccttcca
      661 ggggcgacga tgacgcttgc gctcctgtgg acggttccgt cggcaaggac gacgccgcag
      721 gcgcgtccgt tctctatcag gagatcctcg acgaacgttc ccatcaaaat gtcgcacttc
      781 ggcttcagcg cctcgtacat attgttgagc acttcgcgcg aggcgtccgt gccgatatgg
      841 cggatcctcg cgggcagaat cttgatcccg acgctggagg ccttcctgat ggtatcgcgg
      901 acgatgtcgc ccgagggttc gtacgcatgg ttgtcggcgc cgtactcgac gaagacgcta
      961 tccacatgcg cgatcatgtc gagaagcgct tcctcgccga tactctcctc aaggttgccc
     1021 ccaaaacccg tggtcagcgt caactttccg tcggatgccg aacccgcgcc gccccacccc
     1081 gagacgatac tgcacgagga gcagttgagg caggtcttgc tcgtcccctc gacaatcggg
     1141 cacttgcgct cgcggatcaa acgtcccttg tcgatgatca gcaccttttt ttcatgccgc
     1201 acgcactcca tggcggcgaa aattccggca gggccggaac ctactataat gacgtcgtac
     1261 attgtgagac gcacttcctt tcatagcgtc ctcaaaaaag cgccggagct cttcgcctgc
     1321 cgtcggacgg caagcggctt caaaaagaga cgaaggcgtt accgcccgct tctgtcatat
     1381 ttcgactttc ctctgacatt tttccggagc ttccggagta tttcgagatt cctcgcaatc
     1441 tcgtcggcgt tcccttcgcc tgcaaacgat tcgacctgta tctttcccat cttcacaagc
     1501 aggttgatcg ccttcgtatc tatccccagt tcatcggcaa gctgctgtat cgagattttc
     1561 cggtgtctgc ccgcctccgc agtgttgtcc cgcagatagc cccatgcgag catgtaaata
     1621 ccctgcacct cctgagcaca cggaggacag agcgctacct gcttctcccc gccctcgtcg
     1681 aaccatgcct tgccgcaata ctcgcatact ctcaacgcca tagcgtcagc ctcccgtcgt
     1741 taccggcgcc tccatatcca atgcttccgt cacttcctcg atgaaacgct ccactctttc
     1801 gggagtgtga atgaacgaag gatcgcccaa atccagggtt atgcagcgcg tcgccgagag
     1861 tcctatctcc gagcgtacaa aatcgaggaa agacgcatac gcgtcgtaga gcgaacgcca
     1921 gtactcctcg cccgcctcta tctcgtcgga gcgcccccgc atacggatgc gccgcatgat
     1981 ctcctcgaac ggacaggaga gcacaatgat cagcttgggc atggccagat gcttcagaag
     2041 cgaatcgtac agcatcaggt acgttccgaa ctggctgtcc cggaaatagc ccagcttgta
     2101 gtagagcgtc gcgtagatct tgtcgccgaa gacgctgcgg tcgagaatat actcgtcgga
     2161 ttccgacgcg cagagatact gggcgaacct cgtcacgagg aagttgagct gcatagggaa
     2221 agcccaacgc cgttccaggt gaaaacggcg cagcagatcg tactgatccc ggaactcctc
     2281 cggcatgacg cgcagtccaa gacgctcgga gaggagattc accactgtcg atttgccgct
     2341 tgccgtcata ccctcaacaa taatctgcac cctgcccata ttcttcctcc tccggttgta
     2401 tcatccccga cgtttccggc gcgaaaaggg cgacgctacg tcaggggacg aagggcgcga
     2461 ggatcgcttt gtttcccccc gccttttttg cctcggcgag cgcgtcctcc gctcttttta
     2521 cgaattcatt catgtccatg ctccgacgaa actcggcaac ccccatgctg gaggagagta
     2581 ttctatcggg aaggaatctg tagttgtaga tcatgtcgcg tatcgcctcc gcaatctgga
     2641 aggcgatacg tccgctcatc ggcgtcagta cggcgaattc gtccccgccc cagcgtccga
     2701 tgaaatcagt gggacggatg cggctcttga cggcggcgca gaagtccttg aggactctgt
     2761 cgccggcttc cc
//
LOCUS       DAOTIL010000309         2756 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminobacteriaceae bacterium isolate AMR_MDS_3026
            NODE_43488_length_2756_cov_4.452425, whole genome shotgun sequence.
ACCESSION   DAOTIL010000309 DAOTIL010000000
VERSION     DAOTIL010000309.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868050
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminobacteriaceae bacterium (wastewater metagenome)
  ORGANISM  Aminobacteriaceae bacterium
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae.
REFERENCE   1  (bases 1 to 2756)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2756)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:19:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,224
            CDSs (total)                      :: 3,198
            Genes (coding)                    :: 3,184
            CDSs (with protein)               :: 3,184
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2756
                     /organism="Aminobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_43488_length_2756_cov_4.452425"
                     /isolate="AMR_MDS_3026"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:3034609"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(181..1119)
                     /locus_tag="P5201_07250"
     CDS             complement(181..1119)
                     /locus_tag="P5201_07250"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRV98366.1"
                     /translation="MIFELMSKGNLAQLPDEMCVVGLPETPSPRVLLLLPYNRYLLGT
                     VSLKTYEHWRLGKLGSEESEELILYDDKPKTITLGEVETITIAKEVVDRLKSCLRNQM
                     PPPGEHLPAMLILRGLLREDALFQDEDYLKNENAMQESLDKDAPARMAYWAIRFALSR
                     NDYEGVSRIKTWLKIAPDVFEGTLQTPRIWFSLMDLPGRKDIEEMESLAFSVDDLQRM
                     NSQSSRPVVLYSKAGYLILSDFGSDDAAFRIWLYLPIPLWNEMREKRKLSIREIVMAT
                     WGYLDGLAADTERSSFGLLASMTGGSGIGKATQGLS"
     gene            complement(1244..2092)
                     /locus_tag="P5201_07255"
     CDS             complement(1244..2092)
                     /locus_tag="P5201_07255"
                     /inference="COORDINATES: protein motif:HMM:NF015251.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HRV98367.1"
                     /translation="MRGKAAVLDAIAAFSRPGWRARTVAAFFSGGLKCIRPRNAVARE
                     NLRMVFPEASEEWIRDIVKKLYEHLAWMVAEYLALLREPQQVLEWCEYVEGKAVLDEL
                     KQAGRGAIILTGHVGNWELLAAWLAQSGYPLTAVVRNPDDPNLSEMITAYRTRMGVGS
                     FEKHFVMKEAVRFAKRGGFLGLLPDQAWNATGVMTRFLGHNCYTAGGPAAMAHLARVP
                     VVPVVSYRIAPFRHKVIISSPVPMAEGLDRDDSIQENTERMNKIIEEMILRAPEQWLW
                     LHRRWK"
     gene            complement(2089..>2756)
                     /locus_tag="P5201_07260"
     CDS             complement(2089..>2756)
                     /locus_tag="P5201_07260"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRV98368.1"
                     /translation="WHLWGTPPSWWKFIRLRSRTIHTLFENPAKDWKGHPTVLSATAC
                     SSGESYIPPAFEVKMNWSADEGKESFEETGTPVFLLSGGSAEEFEVFAEGAAKASSTL
                     RNFDESPDEGMKLLSSGNCILLLPRPSSSLALLSAFVALMGIPSVAVRSALMDEMLGK
                     EGYVHLQSENPAEAAHALRSALGEGGRSASAFARRNITDTFSPEKGARKLKTLYLSLG
                     GDQ"
BASE COUNT          671 a          765 c          651 g          669 t
ORIGIN      
        1 accattgtaa gctccgtacg catcttttga aacgtttatc atgatcggca tcatctactt
       61 ggtaatgacc agcatcacca gccttgctct tcaatatatt gaaaagcgat ggaccgtaga
      121 ttgataactg ataccaagaa aaaggctgtg cctcatcttc cggcacagcc tttttctttc
      181 tcaggagagc ccctgagttg cttttcctat gccgcttccc cctgtcatcg aggccaaaag
      241 accgaaggaa gagcgttccg tatcggcggc aagtccatcc agataccccc acgtcgccat
      301 aacgatttcc ctgatcgata gctttctttt ctcccgcatt tcattccaca gcgggatagg
      361 aaggtagagc cagatacgga atgccgcatc gtcgctgcca aaatccgaga gtatgagata
      421 ccccgctttg ctgtagagca cgaccggcct ggaagactgt gaattcatcc gctggaggtc
      481 atccacggaa aaggctaggc tttccatctc ctcaatatct ttccttcctg gtaaatccat
      541 cagtgaaaac caaatgcgcg gggtctgcag cgtgccctcg aaaacatcag gagctatttt
      601 cagccacgtt tttatcctgg aaacgccttc atagtcgttt cttgaaagag caaagcggat
      661 ggcccagtag gccattcgtg ccggagcgtc tttatcaagc gactcctgca tggcgttctc
      721 attctttaga tagtcctcgt cctgaaaaag cgcgtcctcc ctgagcagtc cgcgaaggat
      781 aagcatggcc ggcagatgct ccccgggagg aggcatttgg ttacggagac agcttttcaa
      841 gcggtctaca acctcttttg caatcgttat cgtttcaact tcgccgagcg tgatagtctt
      901 cggtttatca tcatagagga tgagctcctc cgactcctcc gaacccagtt tcccaagcct
      961 ccaatgttcg taggttttta aagaaacagt accgaggaga taacgattat agggaaggag
     1021 gaggaggacc ctcggagaag gagtttcggg caagccgacc acacacattt catccggcag
     1081 ttgtgcaaga tttcctttcg acataagctc gaatatcata tacatccccc ccttttaaaa
     1141 atagagtagc attatttcct aaaagcatca agagaagaat accacatccc cctttttatt
     1201 tcgcaatttt cttttttcag cattccggaa cgaccgtttt gttctatttc cagcggcgat
     1261 gcaaccaaag ccactgttca ggagcacgta aaatcatctc ttctataatt ttgttcattc
     1321 tctctgtgtt ttcctgaatg gagtcatctc tgtcgagccc ctccgccatg gggacaggag
     1381 atgagatgat aaccttatgg cggaaaggag cgattctgta ggaaacaacg ggtacaacag
     1441 gtacccgggc aagatgcgcc atagccgccg ggcctcccgc tgtgtaacag ttatgtccca
     1501 ggaaacgcgt catcactccg gttgcgttcc atgcctgatc gggcagcaat cctaaaaatc
     1561 cacctctttt tgcaaatcgg acagcttcct tcataacaaa gtgtttttca aaagagccaa
     1621 cacccattcg agtccgatac gctgtgatca tctctgaaag attcggatca tccggattgc
     1681 gaacaacggc agtaagcgga tatcctgatt gtgccagcca agcggcgagg agttcccaat
     1741 tccctacatg accggtcagg ataattgctc ccctcccagc ctgcttcagc tcatcgagaa
     1801 ctgctttgcc ctcgacgtat tcgcaccatt caagcacttg ttgaggctcc cgaagaagag
     1861 caaggtactc cgccaccatc cacgccaaat gctcgtacag tttttttacg atatcccgaa
     1921 tccactcctc cgaggcttcc ggaaacacca tacgcagatt ctccctggca acagcgtttc
     1981 gggggcggat acatttcaat ccgcccgaaa agaaggcggc gacggtacga gcgcgccacc
     2041 ccggacggct aaaagctgca atggcgtcga ggacggctgc ctttcctctc actgatctcc
     2101 tccaagagag agatataggg tctttagttt ccttgctcct ttttcagggg agaaagtgtc
     2161 cgtaatgttg cgccgcgcaa aagcggaagc gctcctgcct ccttcgccca atgccgatcg
     2221 aagagcatgc gcggcttctg cgggattctc cgactgcaga tgaacgtacc cctctttccc
     2281 aagcatctcg tccatcagtg cggagcgaac ggctaccgag gggatcccca tcaaagcgac
     2341 gaaagccgaa agcagagcaa gcgaagatga ggggcgtgga aggagcagaa tgcagttgcc
     2401 ggaggagagg agtttcatcc cttcatccgg cgattcgtca aagtttcgga gagtgctgga
     2461 cgctttcgcc gctccttctg caaacacctc aaactcttcg gcgctacctc ccgagagaag
     2521 aaaaacaggg gtcccggttt cttcaaagga ttcttttccc tcgtctgcgc tccagttcat
     2581 cttcacttcg aaggcaggcg ggatatagct ttctcctgaa gaacatgccg tggccgagag
     2641 tacagtggga tgccccttcc agtctttcgc cggattttcg aaaagcgtat ggattgtcct
     2701 cgatcgtaac ctgatgaatt tccaccacga gggcggagtt ccccaaaggt gccaca
//
LOCUS       DAOTIL010000310         2745 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminobacteriaceae bacterium isolate AMR_MDS_3026
            NODE_43728_length_2745_cov_2.766171, whole genome shotgun sequence.
ACCESSION   DAOTIL010000310 DAOTIL010000000
VERSION     DAOTIL010000310.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868050
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminobacteriaceae bacterium (wastewater metagenome)
  ORGANISM  Aminobacteriaceae bacterium
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae.
REFERENCE   1  (bases 1 to 2745)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2745)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:19:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,224
            CDSs (total)                      :: 3,198
            Genes (coding)                    :: 3,184
            CDSs (with protein)               :: 3,184
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2745
                     /organism="Aminobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_43728_length_2745_cov_2.766171"
                     /isolate="AMR_MDS_3026"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:3034609"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..583)
                     /gene="kdsB"
                     /locus_tag="P5201_07265"
     CDS             complement(<1..583)
                     /gene="kdsB"
                     /locus_tag="P5201_07265"
                     /EC_number="2.7.7.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013048870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008690 - 3-deoxy-manno-octulosonate
                     cytidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0009244 - lipopolysaccharide core region
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-manno-octulosonate cytidylyltransferase"
                     /protein_id="HRV98369.1"
                     /translation="MNILGVIPARYASTRLPGKPLADICGKTLIRRVYERAALSPLLD
                     RLVVATDDEWILEEVRSFGGEAVLTSSDHPNGTCRVAEAAEKYEGDIVLNIQGDEPLL
                     DPLMVDEVAQALLDDDSALSSTLCVPITERLMLDDPSVVKVVRDLRGFALYFSRYPIP
                     YIRSDADAVPVYEHIGIYGFRRDFLNIYVSLPPT"
     gene            complement(580..1827)
                     /locus_tag="P5201_07270"
     CDS             complement(580..1827)
                     /locus_tag="P5201_07270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005658886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRV98370.1"
                     /translation="MTMNEQLLKEFYSGLDDRSKESLDAAAEKIVEAKRRGGKVVVAT
                     GSGPNIHEGVTTLIAELMEKGLVDGVTTSSAVVGHEMGGALDLVKMCSASALGFEERF
                     MPRGDVFEFTQLDEERMNALRAEMVLDEELLAKGERAEGRLVIKAAGNMAYPMGLRNE
                     NLAGEILVLCKALGLPFETVAGWGADRRTMLGAGAALGLPVLVSIPQMVGSGHIGMAI
                     GDSISVFERSRRIAAMLESADVIIESAVALTQEIHDGPFECYTGHGIWSWWKGLPVYS
                     LRDKTLIRIDLDENLRKARDLETGGSLIQEAINQGLPKTKISKIPFRMEMSAFARHEG
                     SIPVIGDIGQVWPVLAWKVAGALGISLDFMSYAQHTTEGKAMREWIVREVQPVDREKI
                     LARARKCGISAAPFGNVETVAKP"
     gene            complement(1824..>2745)
                     /locus_tag="P5201_07275"
     CDS             complement(1824..>2745)
                     /locus_tag="P5201_07275"
                     /inference="COORDINATES: protein motif:HMM:NF016311.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="glycosyltransferase N-terminal domain-containing
                     protein"
                     /protein_id="HRV98371.1"
                     /translation="ETEIWPNMLFSLARRGVPAFLVNGRFSERTARTMRKHPSFWRMV
                     YDCFTLLLVRSETDKEMLLSLGVPAERVRVTGDCKVDALLLRRRNACLGPLEALFGVR
                     DLPHDDAKRRPERDPLFLAGSTHEGEEAIVLDAFMHARRVFSSARLVIAPRHPERADG
                     VRALASEVAPSALLSDVERQTASSRPDIVVVDRIGVLFDLYGVVDAAFIGGSLVDKGG
                     QNILEPAAFGTPLSHGPYMRDFVEAAKDLGALGIATRVGDASEIAAHWEASMKPERRA
                     AVRESAERYFRASGGAARLSADEINGVILR"
BASE COUNT          516 a          913 c          821 g          495 t
ORIGIN      
        1 gcgtcggagg gagggagacg tagatgttta aaaagtcccg ccggaagccg tagatgccaa
       61 tatgttcgta caccggcacg gcgtccgcgt ccgaacgaat atacgggatg ggatacctgc
      121 tgaagtagag cgcgaatccg cgaagatccc gcaccacctt cactacggat ggatcgtcca
      181 gcatcaagcg ttccgttata ggaacgcaga gcgtcgagga gagcgccgaa tcgtcgtcca
      241 gaagcgcttg cgcgacttca tccaccataa ggggatcgag cagcggctcg tccccctgga
      301 tattcagtac gatatcgccc tcgtactttt ccgccgcctc ggcgacccgg cacgtcccgt
      361 tggggtggtc ggacgaggtg agcacggcct ccccgccgaa cgagcggacc tcttcgagaa
      421 tccactcgtc gtcggtggca acgacaagac ggtcgagcag cggcgaaagc gcggcgcgtt
      481 cgtagactct ccggatgagc gtcttcccgc aaatatccgc cagcggtttg ccgggaaggc
      541 gggtcgaggc gtagcgggcg gggatcacgc cgagaatgtt catggcttgg cgacagtctc
      601 cacgttcccg aacggcgcgg cggaaatgcc gcacttccgc gcgcgggcaa gaatcttctc
      661 ccggtcgacg ggctgcactt cccgtacgat ccattcgcgc atcgccttcc cctccgtggt
      721 gtgctgggca tagctcatga aatcgagcga gatgccgaga gcgcccgcaa ctttccacgc
      781 gaggaccggc cagacctgcc cgatgtcgcc gatgacgggg atgctcccct cgtggcgcgc
      841 gaaggcggac atctccatcc tgaacggaat cttggagatc ttcgttttcg gcaacccctg
      901 gttgatcgcc tcctgtatca gcgagccgcc cgtctccagg tcgcgcgcct tgcggaggtt
      961 ctcgtcgagg tcgatacgga tgagcgtctt gtcgcgcaaa gagtagacgg ggagcccttt
     1021 ccaccacgac cagatgccgt ggccggtgta gcactcgaag ggaccgtcgt gaatctcctg
     1081 cgtgagggcg accgccgatt caatgatgac gtccgcggat tcgagcatcg ccgcgatgcg
     1141 gcgggaacgc tcgaagaccg agatgctgtc cccgatggcc atgccgatgt ggccgcttcc
     1201 gaccatctgc gggatgctca caagtacggg aaggcccaaa gccgcccctg cgccgagcat
     1261 ggtccgccgg tcggcgcccc accccgcgac ggtctcgaag ggaagtccca gcgccttgca
     1321 gagcaccaga atttctccgg caaggttctc gttccgcagc cccatggggt atgccatgtt
     1381 ccccgccgcc ttgatcacga ggcgtccctc ggcgcgctcc cccttcgcca gcagctcctc
     1441 gtcgagaacc atctcggcgc gaagcgcgtt catccgctct tcgtcgagtt gcgtgaattc
     1501 gaagacgtcg ccgcggggca tgaaacgctc ctcgaacccg agggccgagg cgctgcacat
     1561 cttcacaagg tcgagcgcgc cgcccatctc gtggccgacg accgcagaac tcgtggtcac
     1621 gccgtcgacg agccccttct ccatcagttc ggcgatgagc gtcgttaccc cttcgtggat
     1681 gttcgggccg ctgcccgtcg ccacgacgac ctttccgccg cgccgcttcg cctcgacgat
     1741 cttttccgcc gccgcgtcga ggctctcctt ggagcggtcg tcaagtccgc tgtaaaactc
     1801 cttcagaagc tgttcgttca ttgtcatcgc agtatcactc cgtttatctc gtctgccgag
     1861 agccgggcag ctcctccgga agcgcggaag taacgctccg cactctcacg gactgctgcc
     1921 cggcgctccg gtttcatact tgcttcccag tgcgcggcaa tctccgaggc gtccccgaca
     1981 cgggttgcta tacccagagc tcccagatcc ttcgccgcct cgacgaaatc cctcatgtag
     2041 ggcccgtggg agagcggcgt cccgaaggcc gccggttcga ggatgttctg tccgcccttg
     2101 tccacaaggc ttccgccgat gaacgcggcg tccacgacgc cgtacaggtc gaagagcacg
     2161 ccgatacggt ccaccaccac gatatcaggt cttgaggatg ccgtctgccg ctccacatcc
     2221 gaaagcaaag cgctcggcgc aacctccgaa gccagtgcgc gcacgccgtc cgcccgttcc
     2281 ggatgcctcg gcgcaatcac caggcgtgca gaggaaaaga cgcgccgagc atgcataaag
     2341 gcatcgagca cgatcgcctc ctcgccttca tgggtgcttc cggcgagaaa gagcggatca
     2401 cgctccgggc gcctcttcgc gtcatcatgc ggaagatccc gaacgccgaa aagagcttcg
     2461 agaggcccga gacacgcatt tctcctgcga agcaagagcg catccacctt gcagtctccg
     2521 gtgacacgaa ccctctccgc gggaacgccg agtgaaagaa gcatctcttt gtcggtctcc
     2581 gaacggacga ggagcagcgt gaaacaatca tacaccattc tccagaaaga tggatgcttc
     2641 cgcatggtgc gtgcggtgcg ctccgaaaag cggccgttta cgagaaacgc cgggacgcct
     2701 ctgcgggcga gcgaaaagag catgttcggc cagatctcgg tctcc
//
LOCUS       DAOTIL010000311         2744 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminobacteriaceae bacterium isolate AMR_MDS_3026
            NODE_43745_length_2744_cov_3.604686, whole genome shotgun sequence.
ACCESSION   DAOTIL010000311 DAOTIL010000000
VERSION     DAOTIL010000311.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868050
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminobacteriaceae bacterium (wastewater metagenome)
  ORGANISM  Aminobacteriaceae bacterium
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae.
REFERENCE   1  (bases 1 to 2744)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2744)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:19:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,224
            CDSs (total)                      :: 3,198
            Genes (coding)                    :: 3,184
            CDSs (with protein)               :: 3,184
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2744
                     /organism="Aminobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_43745_length_2744_cov_3.604686"
                     /isolate="AMR_MDS_3026"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:3034609"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..607
                     /locus_tag="P5201_07280"
     CDS             <1..607
                     /locus_tag="P5201_07280"
                     /inference="COORDINATES: protein motif:HMM:NF012382.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HRV98372.1"
                     /translation="VEQLNAIAELSVKKGFFVFSDEMYRGLEHNPADRLPSAPSIIRR
                     SVALGGLSKAYGLPGLRTGWVTSTDKWLLDRIAALKDYTTICTSAPSEILAIAALRKS
                     DELTGRCRGIVKENLEMARSFFQENSGRFEWFEPHGSSVAFPRLLGGQPVKDFCERAV
                     TERSLLVLPDVVFDVDWNHFRIGLGRRSFPEALSVLREMLN"
     gene            857..>2744
                     /locus_tag="P5201_07285"
     CDS             857..>2744
                     /locus_tag="P5201_07285"
                     /inference="COORDINATES: protein motif:HMM:NF012245.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0098561 - methyl accepting chemotaxis
                     protein complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HRV98373.1"
                     /translation="MLKNLKIGVKLAGGFSLLLVIFAAAGWFAWKNMNEVIAETGTLE
                     ERYLPGIHLSNEVENLGQAVMLNVRTYVLGESFADLDAAKKNFEVLLAVLARGKEHAS
                     KHPGLEQLAEGMNTAEEQVTLYQQLLVESQGHMANIATQRRHAAEAAASMGAVAYDLL
                     FDEKDELEKETEEGAGADTLLDRQESILLLDDLVEAVHKAESYTLRSIAERRIELLDR
                     VEDELKRVESLLKKVQATAVKQRQKDQLKKGFEDVESYKGAIASFREAWKDLDEMGKT
                     RTAAAEGVLAAAAKVMNFSVERTLQIAGVVTGQARDVSGMLIASVLFALFAGIVVAFL
                     ITRMITRPLHRAVALAERAGGGDLTIERKDFGHEGKDEIGILADALAMMVKNQADSVR
                     NVVAAAHEISQGAGALAALSQETNASVEEVRSSLEQVASISESNSAALEESNAGVEEV
                     AAGAQASAKASSEGVAAAEHTAAVAKEAVDRVADVIEDVHSVGGKSQESVEKIRALAA
                     SVENISRFVSVISSIADQTNLLALNAAIEAARAGDAGRGFAVVADEVRKLAEESNRAA
                     REVAAIISGLDESARESIVATEEAERIMSRTAVKADDVRQRLAGVLTEIGNISGVISH
                     IASVA"
BASE COUNT          552 a          741 c          904 g          547 t
ORIGIN      
        1 cgtggagcag ctgaacgcca tcgcggaact ttcggtgaag aagggattct tcgtcttctc
       61 cgacgagatg taccgggggc ttgagcacaa ccctgcggat cgtcttccct cagcgccgtc
      121 aatcattcgc agaagcgtcg ccttgggcgg tctctccaag gcgtacggtc tgccggggct
      181 gcggacagga tgggttacgt cgacggacaa gtggcttctg gacaggatcg ccgccttgaa
      241 ggactacacc acgatctgca cttcggcgcc ctctgaaatc ctcgcgatcg ccgctcttcg
      301 caagagcgat gagcttaccg gccgctgccg cggcatcgta aaggagaatc ttgagatggc
      361 gcgctccttt ttccaggaaa acagcggacg gttcgagtgg ttcgaacctc acggaagctc
      421 ggtcgccttc ccccgccttc tcggcggaca accggtgaag gatttctgcg aacgcgccgt
      481 gaccgagcgt agtctgttgg tattgcccga cgtcgtcttc gacgttgatt ggaaccattt
      541 caggataggc ctggggagac gctcgttccc cgaggctctc tctgttctgc gcgagatgct
      601 caactgattt ttcccaaagc cccccgcctt gagcaagggg ctttcttccg atagttgtct
      661 tccgatcgta gcatgggacg atgcataatt gtaaaaaata acggacgagt tttaacgtga
      721 agcaaagaac aacgccgaag actttcggcg tttttttgat atccctcttt atgctatagt
      781 aacgggcggt cgcattgcgg gcgtttgcag cggagggaag ttcggttgtt attccattca
      841 agaagaggtg agggttttgc tgaaaaattt aaagatcggc gtgaagctcg cgggaggttt
      901 ttccctcctt ctggtcattt ttgccgcggc cggatggttt gcctggaaga acatgaacga
      961 ggtcatcgcg gagacgggta cgcttgagga gcgctatctt cccggcattc atctttccaa
     1021 cgaagtggag aatctcgggc aggcggtgat gctgaacgtc cgaacctatg tcctcggaga
     1081 gagttttgcc gatcttgacg ccgcgaagaa gaacttcgag gtccttcttg ccgtgctcgc
     1141 tcgtggtaag gagcacgctt cgaagcaccc cgggctggaa cagcttgccg aagggatgaa
     1201 tacagccgag gagcaggtca cgctctatca gcagctcctt gtggagtcgc aggggcatat
     1261 ggcgaacatc gccacgcagc gcaggcatgc ggcggaagct gccgcttcca tgggggccgt
     1321 cgcctacgat ctccttttcg acgagaagga cgaactggag aaggagaccg aggagggagc
     1381 cggcgccgac acgctgctgg accgccagga gagtatcttg ctcctggacg accttgtcga
     1441 ggcggtgcac aaagcggaga gctatacgct ccgttctatc gccgaacggc ggatcgagct
     1501 gttggatagg gttgaagacg aattgaagcg ggtcgagtcg ttgctgaaga aggtgcaggc
     1561 gacagccgtc aagcagaggc agaaagacca gctgaaaaag ggctttgaag acgtggagtc
     1621 gtacaagggc gccatcgcgt ccttcaggga agcatggaag gatctggacg agatgggaaa
     1681 gaccaggacg gccgcagccg agggagtgct ggcagcggcg gccaaggtga tgaacttcag
     1741 cgtggagcgc acgctgcaga tcgccggagt cgtgaccgga caggcgcgcg atgtctccgg
     1801 gatgctgatt gcttcggtgc tttttgcgct ctttgcgggc attgtcgtcg cgttccttat
     1861 cacgcgcatg attacccgtc ctctacacag ggcggtcgcg cttgcggaga gagcaggcgg
     1921 cggggatctt accatagagc ggaaggactt cggtcatgaa gggaaggatg aaatcggcat
     1981 actggccgac gcccttgcca tgatggtgaa aaaccaggcg gattcggtcc gcaatgtggt
     2041 cgccgccgcc catgaaattt cccagggcgc cggcgccctg gccgctctgt ctcaggagac
     2101 gaacgcctca gtggaggagg tgcgcagttc gctggagcag gttgcctcga tctccgaaag
     2161 caactctgcc gcgctggagg aatcgaacgc cggggtcgaa gaggttgccg ccggggcgca
     2221 ggcgtccgcg aaggcctctt ccgagggcgt tgccgcggcg gaacatacgg cggcggttgc
     2281 aaaggaggcg gtggaccggg tggccgacgt catcgaagac gtccactcgg tcggcggcaa
     2341 gtcgcaggag agtgtggaga agatccgcgc cctcgcggct tcggtggaga atatctctcg
     2401 ttttgtctcc gtcatcagct ccatagcgga tcagacgaat cttctcgcgt tgaacgccgc
     2461 tatcgaagcc gccagggcag gagatgccgg gcgcgggttc gccgtcgtcg ccgacgaagt
     2521 gaggaagctt gcggaggagt cgaacagggc tgcgcgcgaa gtggccgcaa tcatctccgg
     2581 gcttgacgag agcgcgcgcg aatccatcgt cgcgaccgag gaggcggagc gcatcatgtc
     2641 ccggacagcc gtgaaggcgg acgacgtcag gcagcgtctc gccggcgttc ttaccgagat
     2701 cggcaatatc tccggtgtga tctcgcacat cgcctccgtg gcgc
//
LOCUS       DAOTIL010000312         2741 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminobacteriaceae bacterium isolate AMR_MDS_3026
            NODE_43792_length_2741_cov_3.950484, whole genome shotgun sequence.
ACCESSION   DAOTIL010000312 DAOTIL010000000
VERSION     DAOTIL010000312.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868050
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminobacteriaceae bacterium (wastewater metagenome)
  ORGANISM  Aminobacteriaceae bacterium
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae.
REFERENCE   1  (bases 1 to 2741)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2741)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:19:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,224
            CDSs (total)                      :: 3,198
            Genes (coding)                    :: 3,184
            CDSs (with protein)               :: 3,184
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2741
                     /organism="Aminobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_43792_length_2741_cov_3.950484"
                     /isolate="AMR_MDS_3026"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:3034609"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(37..822)
                     /gene="lpxA"
                     /locus_tag="P5201_07290"
     CDS             complement(37..822)
                     /gene="lpxA"
                     /locus_tag="P5201_07290"
                     /EC_number="2.3.1.129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014162704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008780 -
                     acyl-[acyl-carrier-protein]-UDP-N-acetylglucosamine
                     O-acyltransferase activity [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-ACP--UDP-N-acetylglucosamine
                     O-acyltransferase"
                     /protein_id="HRV98374.1"
                     /translation="MPFIHPTALVSSKAELADDVFVGPYCIVHDRVRIGSGTVLSSFV
                     TLLDFVETGKNCRFEQHVVLGGEPQDHAFNGEESWVRIGDAVTLRENVTVHRATGEGA
                     CTVVGDESLLMEGVHVGHNAVVGKRATLANKVGLSGFSSVGDGAVMGGMSGIHQFVRV
                     GALCMIGGLSKIVKDIPPYLMADGHPADIYGLNKVGMRRAGFGASARSAVRSLYDELF
                     RGGLPFRQAVERLAASEKGSDPVAREILSFCGESKRGVSLWTN"
     gene            complement(823..1305)
                     /gene="fabZ"
                     /locus_tag="P5201_07295"
     CDS             complement(823..1305)
                     /gene="fabZ"
                     /locus_tag="P5201_07295"
                     /EC_number="4.2.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013048883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-ACP dehydratase FabZ"
                     /protein_id="HRV98375.1"
                     /translation="MLDIQKIMKFLPHRFPFLLVDRILEIDEAHAVGIKNVTINEPFF
                     QGHFPSEPVMPGVLVLEAMGQVAAMMLSARPGSENLITFLTGVEKAKFRRPVKPGDQL
                     VTTAEMLKLRGKMGKVRTVGRVDGAVAAEAEFSFVAAETLAASEEKAAPSRSGEEAAR
                     "
     gene            complement(1330..2199)
                     /locus_tag="P5201_07300"
     CDS             complement(1330..2199)
                     /locus_tag="P5201_07300"
                     /EC_number="3.5.1.108"
                     /inference="COORDINATES: protein motif:HMM:NF015299.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008759 - UDP-3-O-[3-hydroxymyristoyl]
                     N-acetylglucosamine deacetylase activity [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-3-O-acyl-N-acetylglucosamine deacetylase"
                     /protein_id="HRV98376.1"
                     /translation="MTATRRLLHPVCFSGQGLHTGESSEVVISPLPSGSGPELEIEGA
                     RTPLEICPVSGAGRGTTVSLPHSDLRARTTEHLFAALAVLGLWSVRISLKGPEIPAAD
                     GCSALFAESLYGASAPEKPEEDSSLRMDAFSPAFPVTIVDGEKESSLSLFPWGALSIT
                     YVIQYAERPIGTQMATFDAEKDDFRKYIAGARTFALDTEIERLRAAGLARGGSLDNAV
                     VVGEDAVQALGGLRFPDEFVRHKILDLLGDLYLLGRPLNARITAIRAGHAMHCVLVDR
                     VRRLCRGGSRVHQ"
     gene            complement(2196..>2741)
                     /locus_tag="P5201_07305"
     CDS             complement(2196..>2741)
                     /locus_tag="P5201_07305"
                     /EC_number="2.3.1.191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013048885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016410 - N-acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-3-O-(3-hydroxymyristoyl)glucosamine
                     N-acyltransferase"
                     /protein_id="HRV98377.1"
                     /translation="HSNAVLGCDGFGIAPSAGEAPPEKIPQIGGVVLGDDVELGVCST
                     VDRGTLDDTVIGNGTKVDNHVHIAHNVRIGRNCILVAMTGIAGSAILEDNVIMAAGSG
                     VKEHTRIGKGATVAAKGGAIKDVPPGEVVSGFPARPHRENFRVQAALQRLPETLSRVK
                     KLEKRLDALLPDGRGGSGAGE"
BASE COUNT          564 a          874 c          770 g          533 t
ORIGIN      
        1 tatatcctct tcatgatcct tcccccggaa ggcatgtcaa tttgtccaga gcgaaacccc
       61 ccgcttggat tccccgcaga aggagagaat ctccctggca accggatcgc ttcccttttc
      121 ggaagcggcg aggcgttcga cggcctggcg gaagggcagg cctcctctga agagttcatc
      181 gtagagggag cggactgcgg aacgtgcgga cgcgccgaag cccgcccgcc gcatccccac
      241 cttgttcaga ccgtagatgt cggcagggtg tccgtccgcc atgaggtagg gaggaatatc
      301 cttgacaatc ttcgacaacc ctccgatcat gcagagagcc ccgacacgga cgaactgatg
      361 gataccggac ataccgccca tcacggcgcc gtcgccgacg gaggagaatc cggagaggcc
      421 gaccttgttc gccagggtag cgcgcttccc gacgacggca ttgtgtccga cgtggactcc
      481 ttccatcagc agggattcgt cgccgacgac ggtgcaggcg ccttcgcccg tagcgcgatg
      541 caccgtgacg ttttcacgga gcgtcacggc gtcgcctatg cgaacccacg actcctctcc
      601 attgaacgca tgatcctgcg gctcgccgcc gaggacgacg tgctgttcaa aacggcagtt
      661 tttccccgtc tcaacgaaat cgagcagggt gacgaaagat gagagcactg tccccgaccc
      721 gatccggacg cgatcatgca ctatgcagta gggcccgacg aaaacgtcgt ctgccagttc
      781 ggccttcgat gagacgagag ccgtcggatg gatgaacggc atctaacgag ccgcctcctc
      841 cccgcttcgc gagggggcgg ctttctcttc cgaggcggcg agggtctcgg cagccacaaa
      901 actgaattcc gcctccgccg caactgctcc gtccacacga cctacggtgc gaaccttccc
      961 catcttcccc ctgagtttga gcatctccgc agtcgtcaca agctggtctc ccggctttac
     1021 gggcctccgg aatttcgctt tttccactcc agtgaggaac gtaatgagat tctcgcttcc
     1081 gggacgggcg gagagcatca tcgccgcaac ctgtcccatc gcctccagaa cgagaactcc
     1141 gggcataaca ggctcggagg ggaaatgccc ctggaaaaag ggctcgttga tcgtgacgtt
     1201 tttaataccc actgcatgag cctcgtcgat ttcgaggata cggtcgacga gaagaaaggg
     1261 gaaacggtgc ggcaaaaact tcattatctt ctggatatca agcatagttc gtctcctttt
     1321 cataaaatat cattgatgta ctctgcttcc gccccggcag agtctgcgaa ccctgtccac
     1381 aagaacacag tgcattgcgt gtcctgcacg gatagccgtg atacgggcgt tcaaaggccg
     1441 tccgagcaga taaaggtcgc ccaacaggtc gagaatctta tgccggacaa actcgtcggg
     1501 gaaacgcaag cctcccaaag cctgaacagc gtcctctccc acgacgaccg cgttgtcaag
     1561 actcccgccg cgggcaagcc cggcggcgcg caagcgttct atttccgtat caagcgcgaa
     1621 ggtccgcgct cctgcgatgt atttccggaa gtcgtctttt tctgcgtcaa acgtcgccat
     1681 ctgggttccg atcggccgtt cggcgtactg tatgacatac gtgatcgaaa gcgctcccca
     1741 agggaagaga gagagagagc tctccttctc gccgtcaact attgtcacag gaaacgccgg
     1801 agaaaaggcg tccattcgaa gagacgaatc ctcttccggt ttttccgggg ccgacgctcc
     1861 gtagagcgac tcggcgaaga gggcggagca tccgtcggct gccggaatct ccggcccctt
     1921 gagcgatatc cgcaccgacc agagccccaa cacggcaagc gctgcaaaga gatgctcggt
     1981 cgtacgagcc cgtagatcac tgtgcggaag ggaaactgtc gttccgcgtc cagcgccgga
     2041 aacgggacag atctccaaag gggtccttgc gccttctatc tccagttcag gtccggaacc
     2101 ggagggtaag ggagagatga cgacctccga cgattctcct gtatggagcc cctgcccgga
     2161 gaagcagacc ggatgaagca gacggcgggt cgcggtcatt cccctgctcc gctcccgcct
     2221 ctcccatcgg ggagcagcgc gtcaaggcgt ttttcgagct tctttacacg ggagagcgtt
     2281 tccggaaggc gctgcaacgc cgcctgtacc ctgaagttct ctctgtgagg acgagccgga
     2341 aaacccgaga cgacctcccc cggaggaaca tccttgatcg ctccgccttt cgccgcgacc
     2401 gtagcgccct tccctatccg cgtatgctct ttcaccccgc tgccggccgc catgatcacg
     2461 ttgtcctcaa ggatggcgct gccggcgatt cccgtcatgg cgacgagaat acagttcctg
     2521 cctatgcgga cattgtgcgc gatgtgcaca tgattatcca ccttcgtacc attcccgatc
     2581 acggtatcgt caagcgttcc cctgtccacc gttgagcaga cgcccagctc cacgtcgtct
     2641 ccgaggacga cgccgcctat ctgaggaatt ttctccggcg gcgcctcgcc tgcggaggga
     2701 gcaataccga atccgtcgca cccgagaacc gcattggagt g
//
LOCUS       DAOTIL010000313         2741 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminobacteriaceae bacterium isolate AMR_MDS_3026
            NODE_43795_length_2741_cov_3.785182, whole genome shotgun sequence.
ACCESSION   DAOTIL010000313 DAOTIL010000000
VERSION     DAOTIL010000313.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868050
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminobacteriaceae bacterium (wastewater metagenome)
  ORGANISM  Aminobacteriaceae bacterium
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae.
REFERENCE   1  (bases 1 to 2741)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2741)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:19:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,224
            CDSs (total)                      :: 3,198
            Genes (coding)                    :: 3,184
            CDSs (with protein)               :: 3,184
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2741
                     /organism="Aminobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_43795_length_2741_cov_3.785182"
                     /isolate="AMR_MDS_3026"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:3034609"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..274
                     /locus_tag="P5201_07310"
     CDS             <1..274
                     /locus_tag="P5201_07310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006302045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="HD domain-containing phosphohydrolase"
                     /protein_id="HRV98378.1"
                     /translation="EPIRLHHRWWNGKGYPAGDGGEAIPLESRLMAIVDAYEAMTGDK
                     IYRTPLSPEEALKELHRCAGSQFDPVWTERFITFFREYREREHIFA"
     gene            422..1468
                     /gene="rlmN"
                     /locus_tag="P5201_07315"
     CDS             422..1468
                     /gene="rlmN"
                     /locus_tag="P5201_07315"
                     /EC_number="2.1.1.192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013047628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_function: GO:0016433 - rRNA (adenine) methyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2503)-C(2))-methyltransferase
                     RlmN"
                     /protein_id="HRV98379.1"
                     /translation="MNESVNALDLSYEEWIETSVTRWKLPRYRADQLCQWIYDKKVFN
                     VHEMTNLSKDLREKLVYELMILPPIAVREDTSKDGTKKFLWQLYDGERIESVLLSHGS
                     HMTACISSQVGCPLNCSFCATGKSGFTRNLSAGEIVGQFLAMEKRIGENINNVVYMGM
                     GEPFLNSAEVFKSIRILNSSKMRNLGARHITISTSGVVPGILELADFPIPVRLSVSLH
                     APNDVLRSKIMPVNRKHSLGSLIDALKAYKDKTGERITIEYVMLQKVNDEPEHAYEMA
                     AILSGLGVYVNLIPYNPIALLQPGEQGFQRSTTERIKEFAAILAKLGIENEIRKEKGT
                     DIDAACGQLRAQSM"
     gene            1515..1844
                     /locus_tag="P5201_07320"
     CDS             1515..1844
                     /locus_tag="P5201_07320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013047627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046914 - transition metal ion binding
                     [Evidence IEA];
                     GO_process: GO:0006811 - monoatomic ion transport
                     [Evidence IEA];
                     GO_process: GO:0055072 - iron ion homeostasis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FeoA family protein"
                     /protein_id="HRV98380.1"
                     /translation="MKRPQRKDGNTTLSLRDLPNGSLARIVKILSPLHNAAETGSSCC
                     AARLEALGLRVGKIVEKVSGMPFHGPVTLLLDGRQIAVGWNISSHVLVVPVKERERAH
                     GRSAERE"
     gene            1819..>2741
                     /locus_tag="P5201_07325"
     CDS             1819..>2741
                     /locus_tag="P5201_07325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012869281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FeoB small GTPase domain-containing protein"
                     /protein_id="HRV98381.1"
                     /translation="MDEVRNESKKLLLVGNPNVGKSALFTRITGVHAVSSNYPGTTVG
                     FLEGWLRRSGDVWRVIDVPGAYTLSPTNEAEEVAERMVNEGADVAVLVLDATALERNL
                     FLAFQTMERRLPVIIALNMVDEARHKGIDVDPEALERLLGVPVVPTVAVTGQGISRLL
                     DRLPEARPARIALPGGYASETGVPGREERWSAIGEIIEQVQRVSHRHHTLLDKLEDAS
                     VDSFWGLIIGVLVIVASFASIRFLGEGIIDEFFDPLFEAWLLPVAERLSLFLGGEGLF
                     HDIVVGRLIDGHIDFEQSFGLLTTGLYVPLA"
BASE COUNT          619 a          789 c          792 g          541 t
ORIGIN      
        1 ggagcctatc cgtctgcacc accgatggtg gaacgggaaa gggtaccccg caggagacgg
       61 aggagaggcg atcccgctcg aaagccgcct catggcgatc gtcgacgcct atgaagccat
      121 gacgggagac aagatctaca gaacgcccct ctccccggaa gaagcgctga aagagcttca
      181 ccggtgcgcc gggagccagt tcgacccagt atggaccgaa cgttttatta cgttttttcg
      241 cgagtatcgg gagagagaac atatctttgc atgaacggcc ggcatccgga gagattccgt
      301 accctgcatg ggttttccta gagaattcgg cggaacaggc attgacagag agcatctttc
      361 catgacatga ttcagtgtta gaattgaaac ggcgttcttg aacgcctcag gaggcagcgg
      421 tatgaacgaa agcgttaacg ccctcgatct ctcgtacgaa gagtggatcg agacaagcgt
      481 cacaaggtgg aaacttccac gctacagagc cgaccagctc tgccagtgga tctacgacaa
      541 aaaagtcttc aatgttcacg agatgaccaa cctctcgaag gatctgcggg agaagctcgt
      601 ttacgaactg atgatccttc ccccgatcgc cgtccgcgag gatacgtcga aggacggcac
      661 gaaaaaattc ctctggcagc tttacgacgg agagcggatc gaatccgtcc tgctgtcgca
      721 cggctcgcat atgacagcct gcatctccag ccaggtgggg tgtcctctga actgctcctt
      781 ctgcgccaca ggaaaatcgg gtttcacgcg caacctcagc gccggcgaga tcgtcgggca
      841 gttcctggcg atggaaaaac gcattggaga aaacatcaat aacgtggtct atatggggat
      901 gggcgaacct ttcctgaatt ccgccgaagt gttcaagtcg atccggattc tgaattcgtc
      961 aaagatgcgc aacctggggg cgcgccacat cacaatctcg acatccggcg tcgttccagg
     1021 cattttggaa ctggcggact tcccgattcc ggtgcgtctt tccgtctccc tccacgcgcc
     1081 gaacgacgtg ctgcggagca aaataatgcc ggtgaacagg aagcactctc tgggctcgct
     1141 catcgacgcg ctgaaggcct acaaagacaa gacaggggaa cggatcacga tcgaatacgt
     1201 catgctgcaa aaagtcaacg acgagcctga gcacgcctac gaaatggccg ccattctttc
     1261 ggggctcggc gtctacgtga acctgatacc gtacaatccg atcgcgctcc tgcagccggg
     1321 ggagcaggga tttcagcgtt cgacgacgga gcgcatcaag gagttcgcag ccattcttgc
     1381 caaactcggc atcgaaaacg agatccgcaa ggaaaaagga acggacatcg acgctgcctg
     1441 cggccaactg cgggcgcaga gcatgtaagg gggtcgtcag aagagtgaac ctcctccaac
     1501 gtgtcgcccg ctccttgaaa cgcccgcaaa ggaaggacgg caacacgacg ctttcgttgc
     1561 gcgacctccc gaacggatct ttggcccgga ttgtcaagat cctgtctccg ctgcacaacg
     1621 cagcggagac aggatcttca tgctgtgccg cacggcttga agctcttggg cttcgcgtcg
     1681 gcaagatcgt tgaaaaagtg tccgggatgc cctttcacgg accggtaaca ctgcttctcg
     1741 acgggcgaca gatagccgtc ggatggaata tttcgtcaca cgtactggtt gttcccgtga
     1801 aagaaagaga gcgcgctcat ggacgaagtg cggaacgaga gtaaaaagct tctgctcgtc
     1861 ggcaacccga acgtcggcaa gagcgccctc ttcacccgaa tcacaggcgt tcacgccgtt
     1921 tcgtcaaact accccggcac gaccgttgga ttcctggaag gctggcttcg ccggagcggc
     1981 gatgtgtggc gtgtaatcga cgttcccgga gcgtacacgc tctcgcctac gaacgaagcc
     2041 gaggaagtag cggaacgaat ggtcaacgag ggagcggatg ttgccgtgct tgttctcgac
     2101 gcgacggccc tggaacgcaa cctctttctc gccttccaga cgatggagcg gcgcctgccg
     2161 gttatcatcg cgctcaatat ggtcgacgaa gcccgccaca agggaattga cgtcgatccc
     2221 gaggcgttgg aaagactgct cggcgttcca gtcgtcccta cggtcgcagt gaccggacag
     2281 ggaatcagcc gtctgctgga ccgtctcccg gaagcccgtc ccgccaggat cgcgctcccc
     2341 ggagggtacg cttccgaaac cggcgttccc ggcagggaag agcgctggag cgccatcggt
     2401 gagataatcg aacaggtcca gagagtcagt cacaggcacc acacgctcct cgacaagctt
     2461 gaggacgctt ccgtggactc tttttgggga ctgattatag gagttctcgt catcgtcgcg
     2521 agctttgcct ccattcgctt ccttggagag ggaatcatcg acgaattttt cgacccgctc
     2581 tttgaagcgt ggctgctccc tgtagccgag cgtctttcct tgtttctggg aggggaaggg
     2641 cttttccatg acatcgtcgt cggccgcctc atcgacgggc acatcgactt cgagcagagc
     2701 ttcggcctgc tcaccaccgg tttatacgtc cccctggcga t
//
LOCUS       DAOTIL010000314         2738 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminobacteriaceae bacterium isolate AMR_MDS_3026
            NODE_43875_length_2738_cov_3.811405, whole genome shotgun sequence.
ACCESSION   DAOTIL010000314 DAOTIL010000000
VERSION     DAOTIL010000314.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868050
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminobacteriaceae bacterium (wastewater metagenome)
  ORGANISM  Aminobacteriaceae bacterium
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae.
REFERENCE   1  (bases 1 to 2738)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2738)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:19:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,224
            CDSs (total)                      :: 3,198
            Genes (coding)                    :: 3,184
            CDSs (with protein)               :: 3,184
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2738
                     /organism="Aminobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_43875_length_2738_cov_3.811405"
                     /isolate="AMR_MDS_3026"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:3034609"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..253
                     /locus_tag="P5201_07330"
     CDS             <1..253
                     /locus_tag="P5201_07330"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRV98382.1"
                     /translation="AVASIAVRDAELLLLDEPSRDFDPQWQELFEAWLREHNAAVLAI
                     SHDPDFVERLFPQVWELNQGRLSQRRGREAPFSIDQRNS"
     gene            complement(322..1500)
                     /locus_tag="P5201_07335"
     CDS             complement(322..1500)
                     /locus_tag="P5201_07335"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HRV98383.1"
                     /translation="MLDQLIAVLDLATLEIALGATSLISCATMLALWRTNSSEKGPAC
                     WAAATFLGGSGLFAHFFLLNFIGGAETLRVTLPFVVGSFPLALLLALEGTLRFQGIGG
                     ERKRAYVLPVFAVALLCTSFLGAENASARYMINDPIMILILLLIAFFLLKRTEGLERG
                     IHAIPSFCCIFLAATLAYRCFFAFSGTTESEIHSSSRLVYFAFLLWAPGWTCGLCMAV
                     MFRARRFILKIADHDSLTGLANRRKFDRTADLLLGRKNSIRKKFALLLIDLNGLKQLN
                     DLHGHATGDRALVLMSRALEHTVGPDDIAARFGGDEFVVLTREVKDRDDLERLEQTIR
                     DSVEREHDLDEERKLLLRMSIGSALFPEDGRNTGELLYIADQRMYLEKQTRRSLSKVV
                     "
     gene            1727..2548
                     /locus_tag="P5201_07340"
     CDS             1727..2548
                     /locus_tag="P5201_07340"
                     /inference="COORDINATES: protein motif:HMM:TIGR01460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IIA family hydrolase"
                     /protein_id="HRV98384.1"
                     /translation="MTHPLSDTLRHKKLYLLDMDGTLYLGDRLFPVALPFLDALRAAG
                     ARYLFLTNNSSRGTGSYVQKLGGMGISACEEDFFTSTDAACAYLRAHYSGAKIYVLGT
                     ASFRRHLKQKGFPVTDQLEDDVDCLLMGYDTELTYQKLIDASILLGRNVTYLATNPDW
                     VCPTAFGYVPDCGSIAQALEHATGRLPLFLGKPQPEIALLAMERAGVSPEDTVLVGDR
                     IYTDIACGKRAGVTSILVFSGETTREVLQESDIQPDFALKDVGEITAVLHALSGR"
BASE COUNT          564 a          820 c          775 g          579 t
ORIGIN      
        1 cgccgtcgcc tccatcgccg tccgcgacgc cgagctgctc ttgctcgacg agccgagccg
       61 cgacttcgat ccgcagtggc aggagctgtt cgaggcgtgg cttcgcgaac acaacgcggc
      121 ggtcctcgcg atcagtcacg atccggactt cgtcgagcgt ctcttcccgc aggtctggga
      181 gttgaaccaa ggtcggctca gccaacgccg cggtcgtgag gcgcccttct ctatcgatca
      241 aaggaacagc tgaaagcccc cggtcgccgc cggcccgagc gcacagggcg atccccgggg
      301 ggggcgcact gattcggaag cctacaccac cttggagagc gaccggcggg tctgtttttc
      361 caggtacatc ctctggtccg cgatatacag cagctccccg gtgttgcggc cgtcctccgg
      421 aaagagcgcg ctccctatgc tcatcctcag aaggagcttc cgttcctcgt cgagatcgtg
      481 ctccctctcc accgagtcgc ggatcgtctg ttccaaacgc tccaggtcat cgcggtcttt
      541 cacctcgcgc gtcagcacca cgaactcgtc tccgccgaac cgggcggcga tatcgtcggg
      601 gccgacggta tgttcaagcg cgcggctcat cagcaccagc gccctgtctc ccgtggcgtg
      661 tccgtgaagg tcgttgagct gtttcaagcc gttgaggtcg atgagcagga gggcgaactt
      721 cttccgtatc gagttttttc gcccgagcag caggtcggcc gtccggtcga actttctccg
      781 gttggcgagg ccggtcaggc tgtcgtggtc ggcgatcttc aggataaagc gccgcgccct
      841 gaacatcacc gccatgcaga ggccgcaggt ccaccccggc gcccagagga ggaacgcgaa
      901 gtagacgagc cgggaagaag agtgaatctc gctttcggtc gtcccggaga aggcgaagaa
      961 acagcggtag gccagcgtcg ccgcgaggaa aatgcagcag aacgaaggaa ttgcgtggat
     1021 gccccgttcc agtccttccg ttctcttcaa taggaagaac gcgatgagaa gcaggatgag
     1081 gatcatgata gggtcgttga tcatgtatcg tgcagaggcg ttctccgcac ccagaaaaga
     1141 cgtacataac agtgcgactg cgaacacagg cagtacatac gcccgtttgc gttcgccgcc
     1201 gataccttga aaacggagag ttccctcaag ggccagaagc agtgcgaggg gaaagctgcc
     1261 gacgacgaaa gggagcgtaa cgcgaagggt ttcggcccct cctatgaaat tcagaaggaa
     1321 aaaatgcgca aacagaccgg aacctccgag gaaagtggcc gccgcccagc aggcggggcc
     1381 tttttcggag gaattcgttc tccagagtgc gagcattgtg gcgcatgaga tcagggaggt
     1441 cgccccgagg gcgatttcga gcgtcgccag gtcaagaacg gcgatgagtt gatcgagcat
     1501 tggtcttcgc ctccgcaaat atttgtctga atgtccgttg cgcgccgtcg tggaaaaaat
     1561 aatgcgtcgc aattctatca cttttgatgt gatttcgcgc gggttttttg tactattttg
     1621 tcgcctgcga taaactttcc tttcttttcc ttgccagcca ttgtatttcg ggctattatg
     1681 agtttatttc aacgctcgtg acaaacaagc aaacaaagga gacattatga cacatcctct
     1741 ttccgacacc ctgcgccata aaaagttgta ccttttagac atggacggca ccctgtatct
     1801 gggcgaccgg ctgttccctg tcgccctgcc ctttctcgat gcactgcgcg cggcgggcgc
     1861 ccggtatctc tttttaacca acaactccag ccggggtacc ggcagctatg tgcaaaagtt
     1921 aggcggcatg ggcatttccg cttgcgagga ggattttttc acctccacgg atgccgcctg
     1981 cgcctacctg cgcgctcatt acagcggcgc caagatctac gttctgggca ccgcgtcctt
     2041 ccggcgccac ctgaagcaga agggctttcc cgtcaccgat caactggagg acgacgtcga
     2101 ctgcctcctg atgggctacg atacagagct tacgtaccag aagctgatag acgccagcat
     2161 cttactcggc aggaatgtaa cctacctggc gaccaacccc gactgggtat gccccaccgc
     2221 cttcggctac gtgccggact gcggctccat tgcgcaggcg ctggagcacg ccaccggaag
     2281 gttgcccctc ttcctgggca aaccgcagcc cgaaatcgcc ctgctggcca tggaaagagc
     2341 cggcgtttca cccgaagaca ccgtgctggt gggcgaccgc atctacaccg acatcgcctg
     2401 cggaaaacgc gccggcgtca cctccatcct ggtgttcagc ggcgaaacca caagggaagt
     2461 cctgcaagaa agcgacatcc agcccgattt tgccctcaag gatgtcggtg aaatcaccgc
     2521 cgtgctgcat gcgctttccg gtcgttgaac agctgtattt ttcatgtttt ttctttcttt
     2581 tcttcttgcg gtaatttcct gttcatggta atgtgataac aacatatggg acatgcgccc
     2641 caaaaatcag cgtaaggagg ttctcatcaa catcatgaaa aagctacttg cgttgcttct
     2701 gtgcatcatg ttactgcttc ctgcaacaag cgccctgg
//
LOCUS       DAOTIL010000315         2738 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminobacteriaceae bacterium isolate AMR_MDS_3026
            NODE_43878_length_2738_cov_3.355572, whole genome shotgun sequence.
ACCESSION   DAOTIL010000315 DAOTIL010000000
VERSION     DAOTIL010000315.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868050
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminobacteriaceae bacterium (wastewater metagenome)
  ORGANISM  Aminobacteriaceae bacterium
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae.
REFERENCE   1  (bases 1 to 2738)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2738)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:19:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,224
            CDSs (total)                      :: 3,198
            Genes (coding)                    :: 3,184
            CDSs (with protein)               :: 3,184
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2738
                     /organism="Aminobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_43878_length_2738_cov_3.355572"
                     /isolate="AMR_MDS_3026"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:3034609"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..200)
                     /locus_tag="P5201_07345"
     CDS             complement(<1..200)
                     /locus_tag="P5201_07345"
                     /inference="COORDINATES: protein motif:HMM:NF014591.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047429 - nucleoside triphosphate
                     diphosphatase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Maf family protein"
                     /protein_id="HRV98385.1"
                     /translation="MGGSVNLVLASGSPRRRELLQSLGWAFRIDVPDICEDLLPGEAP
                     AEAASRLSRAKAAGLPVTCGVS"
     gene            complement(185..427)
                     /locus_tag="P5201_07350"
     CDS             complement(185..427)
                     /locus_tag="P5201_07350"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRV98386.1"
                     /translation="MASGSSVKWWIIVASLIIGTISGLYLQRFALTEPFFKDILSTGF
                     SLTDVHLGFADFGLKVWLRWNLGTIIGGVVGIWVAR"
     gene            complement(433..1254)
                     /locus_tag="P5201_07355"
     CDS             complement(433..1254)
                     /locus_tag="P5201_07355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013048846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-diphosphate phosphatase"
                     /protein_id="HRV98387.1"
                     /translation="MSEGLPAFFLGLLQGVTEFLPVSSSGHLALAKQLFSVEHASLTY
                     DVLLHFATMLATLLYFGKDILLLLREWCSGFFSPAARCEEGWTLGWAVLAGTVVTAAI
                     ALPLKPVVERMMELPGAVGAGLLLTSLLLWRGSSIASSLKGAGKPISLGRGFLIGLVQ
                     GVAVLPGVSRSGSTIVAGMSAGLSAPAAFRFSFLLSLPAVFGATLLEVRGLLKTAGWA
                     SSLPAGWIAGFFASFLAGFFSLVLLRRVVSAGKWRPFAVYCAFAGTLSIVLSLLG"
     gene            complement(1251..>2738)
                     /locus_tag="P5201_07360"
     CDS             complement(1251..>2738)
                     /locus_tag="P5201_07360"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013048847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004540 - ribonuclease activity [Evidence
                     IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease J"
                     /protein_id="HRV98388.1"
                     /translation="HGHEDHIGGLPFALKKISAPLYGTKLTLGMVGNKLAEWAPELNP
                     DYREIQAGDVVEMGPFKVRFIAVCHSIPDGVGLAIGTPVGTVVHTGDFKLDPTPIDGR
                     LTEYDAFSEEGKKGVLLLLSDSTNVERPGFTESERVLSGTLDKIFRQYRSKRIVIASF
                     ASNLHRVQQVVDCAARFNRKVAFVGRSMVKNVELARTLGYLHVDDKIIVDMEDISSVP
                     SSSLVVMTTGSQGEPFSGLVLMSRGEHRVISLSDKDVVALFATPIPGNEKLVSGTVNR
                     LFACGCDVVYEKEQGIHVSGHAAKDELRVMLNMVKPKFFVPVHGEYRHLVRHAQLARE
                     TGVATRNAFVLQNGDVFQLDGEKAAIKERVQAGGIMVDGLALGELQGSIMKERRELSE
                     EGLVLVSIVLSGAGELLASPSFESRGFLHLDDAAQLKEDLIASVEKTVLSLGAKSVSD
                     IDLLETKIKGRIRDTLRRFGRSKPMITPLITVLGAPEKNGKGKRK"
BASE COUNT          640 a          894 c          736 g          468 t
ORIGIN      
        1 atcgacacgc cacacgtgac cggcagacca gccgccttcg cccgcgaaag acggcttgct
       61 gcctccgccg gagcttcccc gggaagcaga tcctcgcaaa tatccggaac gtctatccga
      121 aaagcccacc ccagcgattg gagcaactca cgccgcctgg ggctccccga cgcaagcaca
      181 agatttaccg agccacccaa attcccacca cgcctccaat aatcgttccc aaattccatc
      241 gaagccagac tttcaggccg aagtcggcaa acccgagatg cacatccgtc agactgaagc
      301 cggttgaaag aatatccttg aaaaagggct ccgtcaaggc aaatcgttga agatagagtc
      361 ctgaaatagt tcctatgatg agcgaagcga cgatgatcca ccacttcacg gaagagccgg
      421 acgccatcgc attcacccca gcagcgaaag aacgatcgaa agcgtccctg cgaatgcgca
      481 gtacaccgcg aacgggcgcc acttccccgc cgataccacg cgccggagaa gaacaagcga
      541 gaaaaaaccg gcaagaaacg aagcgaaaaa accggcgatc cagcctgccg gcagcgaaga
      601 ggcccagccg gcggttttca ggagtcctcg cacctcaaga agcgtcgctc cgaacacggc
      661 gggcagggaa agaagaaagg agaaacggaa cgccgcaggc gccgacaggc ctgcgctcat
      721 acccgcgaca atcgtcgatc cggaacggga aactcccgga agcaccgcga ctccctgcac
      781 aaggccgatc aggaaccccc ggccgagaga aatcggcttc ccggcgccct tcagcgagga
      841 cgcgatagaa gaacctcgcc acagcagtag cgacgtgaga agaaggcccg cgccgaccgc
      901 tccgggaagc tccatcatcc gctcgacaac gggcttgagc ggaagagcaa tggccgccgt
      961 tacaacggtc cccgcaagta cggcccagcc gagcgtccac ccctcttcgc agcgcgctgc
     1021 cggggagaag aatccggaac accactcacg gagcaggaga agaatgtcct tcccgaagta
     1081 gagcagcgtc gcgagcatgg tagcgaagtg cagcagcaca tcgtaggtca gggaagcgtg
     1141 ctccacggag aagagctgtt tcgccaacgc aaggtgcccg gagctgctca cggggaggaa
     1201 ctccgtaact ccctgcaaca gtcccaggaa aaaggcgggc agaccctcgc tcattttctc
     1261 ttccccttcc cgttcttttc cggagcgcca agaaccgtta taagaggtgt tatcatcggt
     1321 ttacttcgtc cgaagcggcg aagcgtgtcg cgtatccgcc cctttatttt tgtctccagg
     1381 agatcgatat cggagacaga cttcgcgccg agagagagca ccgtcttctc caccgaggca
     1441 atcaggtcct ccttcaactg cgccgcgtcg tcaaggtgca ggaacccccg gctctcgaaa
     1501 gaaggagacg caagcagctc gccggcgccg gaaagtacga tggaaacgag gacaagcccc
     1561 tcttccgaaa gttcccgccg ctccttcatg atacttcctt gaagctctcc gagcgccaac
     1621 ccgtcaacca tgataccccc ggcttgcact cgctctttga tcgccgcctt ctccccgtcg
     1681 agctggaaca cgtcgccgtt ctggaggaca aaggcgttcc gcgtcgcaac gccggtctcc
     1741 cttgcaagct gggcgtgccg gacaagatgg cggtactctc cgtgaaccgg aacgaaaaat
     1801 ttcggcttga ccatattgag catcacgcgc agttcgtcct ttgctgcatg accggagacg
     1861 tgaattccct gctccttctc gtacacaacg tcgcacccgc aggcgaagag gcggttgacc
     1921 gtcccgctca ccagcttctc gtttccaggg ataggagttg cgaagagcgc gaccacgtcc
     1981 ttgtcgctca agctgatcac acggtgctcg ccccggctca tcagcaccag tccggagaag
     2041 ggctctccct ggctccccgt agtcatcaca acgagcgaac ttgaagggac cgaggagata
     2101 tcctccatat cgacgataat cttgtcatcc acgtgcagat acccgagggt ccgtgcaagc
     2161 tcgacgttct tcaccatgct tcttccgacg aaagcgactt ttcggttaaa acgggccgcg
     2221 cagtccacca cttgctgcac acggtgcaga ttgctcgcga acgaggcgat gacgatacgc
     2281 ttcgaccggt actgtctgaa gatcttgtcg agcgttccgg agagcacccg ttccgactcg
     2341 gtgaatcccg gtctttccac atttgtcgaa tctgaaagaa ggagcagcac ccccttcttc
     2401 ccctcctccg aaaacgcgtc gtactcggta aggcgcccgt ctatcggcgt aggatcaagc
     2461 ttgaaatccc ccgtatgcac cactgtgccg acgggcgttc ctatggccag gccaacaccg
     2521 tcgggaatgg agtgacagac cgcgataaaa cggaccttga acggccccat ctccaccacg
     2581 tcgccggcct gaatctcccg gtagtccgga ttcagttcgg gcgcccactc cgcaagctta
     2641 ttgcccacca taccaagcgt cagcttcgtc ccgtagaggg gagccgatat cttcttgagg
     2701 gcgaacggaa gccctccgat gtggtcctcg tgcccgtg
//
LOCUS       DAOTIL010000316         2723 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminobacteriaceae bacterium isolate AMR_MDS_3026
            NODE_44223_length_2723_cov_2.111319, whole genome shotgun sequence.
ACCESSION   DAOTIL010000316 DAOTIL010000000
VERSION     DAOTIL010000316.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868050
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminobacteriaceae bacterium (wastewater metagenome)
  ORGANISM  Aminobacteriaceae bacterium
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae.
REFERENCE   1  (bases 1 to 2723)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2723)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:19:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,224
            CDSs (total)                      :: 3,198
            Genes (coding)                    :: 3,184
            CDSs (with protein)               :: 3,184
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2723
                     /organism="Aminobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_44223_length_2723_cov_2.111319"
                     /isolate="AMR_MDS_3026"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:3034609"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..621)
                     /locus_tag="P5201_07365"
     CDS             complement(<1..621)
                     /locus_tag="P5201_07365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012869316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside hydrolase"
                     /protein_id="HRV98389.1"
                     /translation="MATTAKKPVKIIHDCDPGHDDAVAILMAAVHPMIDLKAVTVVAG
                     NQTLEKTVRNALNVCSASKRCADIPIAAGMSRPMVREQVIAGDIHGESGLDGPVFGEP
                     ELGLDTRHAVDLIIDLLLDSDDPITLVPTGPLTNIAMAIRKEPEIVEKIERIVLMGGA
                     YQHGNVTPSAEFNIYADPEAAHVVFTCGRPIVMMGLDVTRKANATAE"
     gene            complement(689..1624)
                     /locus_tag="P5201_07370"
     CDS             complement(689..1624)
                     /locus_tag="P5201_07370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006583857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HRV98390.1"
                     /translation="MDGLNEILSLVFNATLVRSTIRAATPILYATLACVLTQQANILN
                     IGIEGIMIFAAFAAVTTSYFAGSWVLGLLAAIVTGVVVAAFMAFAHLKAKADIFVVGM
                     GVNMLAIAMTRFLLQRTFGVSGSFHHNDIVPMPRLNFDFGGNALLDTLFNDYSLFEPV
                     GIVLVFVLAFILYKTVWGLRLRSVGLNPLAAETAGINVTFRKFEVMLYSGVLGGIAGA
                     HLSLGYSRMFVENMTNGRGFMGVAAMFFGGANPFWGWIGCLIFGFADSLGSRLQSLGL
                     PSQFILMIPYVVTVVVLTLAMMRKKRAETARKSAI"
     gene            complement(1624..2664)
                     /locus_tag="P5201_07375"
     CDS             complement(1624..2664)
                     /locus_tag="P5201_07375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006583858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HRV98391.1"
                     /translation="MYKTSWGSTLMTIILSLALGAGAILLLGYDPIEAYYQLFRGAFV
                     GKFNIGGVMERFVPLMLTALAFAVSAKVAVFNVGVEGELYLGAIAAAWAGYAFTGLHP
                     LVHIPLCFAVAMVASGAWGAIPGVLKAYWKVNEVCVTILMNYVAIYFTSYLVGNPFSA
                     RASIPRTPTIEESARLLRILRPSRANIGIFVALGVLVLVWWMLYHTNWGYKLRHVGQN
                     PSFSEYVGIPPKKAMIWGMILSGAIGGLAGAIEVMGIHGHFLDNFSPGIAFDGMLASL
                     IAKNDIRMVPVLSLFLAVLKTGALGMERFTGVPKTLIDTIIAVFILLAAMEGLFAYSR
                     RRSARSSGEEAA"
BASE COUNT          543 a          816 c          902 g          462 t
ORIGIN      
        1 ttcggcggtc gcgttcgctt tgcgcgtgac gtcaagcccc atcatgacga tcgggcgtcc
       61 gcaggtgaag acgacgtgcg ccgcctcggg gtccgcgtag atgttgaatt cggcggaggg
      121 ggtgacgtta ccgtgctggt acgccccgcc catgaggacg atgcgttcga tcttctcgac
      181 gatctcaggc tccttgcgga tggccatggc gatgttcgtc agcggccccg tgggaaccag
      241 cgtgatcggg tcgtcggagt caagcagcag gtcgatgatc aggtccacag cgtggcgcgt
      301 gtcaagcccc agctccggct cgccgaagac ggggccgtcg agccccgact cgccgtggat
      361 gtcgcccgcg atcacctgtt cgcgcaccat cggccgggac atgcccgccg cgatggggat
      421 gtccgcgcag cgtttggacg cgctgcagac gttaagggcg ttcctgacgg tcttttcgag
      481 cgtctggttg cccgccacca cggtcacggc cttgagatcg atcatcgggt ggaccgccgc
      541 catcaggatg gcgacggcgt cgtcgtgtcc ggggtcgcag tcgtgaatga tctttacagg
      601 tttcttcgca gttgtagcca ttgcagcacc gccttttctc aaacttccgc gcgggaggcg
      661 cggtgaaaat ccgtttccga aaaaaatgtt atatcgcgct cttccgcgcc gtctccgcgc
      721 gcttcttgcg catcatggcg agggtgagga cgaccacggt gacgacgtac gggatcatca
      781 ggatgaactg cgacggcagt cccaaagatt gcagccgcga gccgagggag tcggcgaagc
      841 cgaagatcag gcagccgatc cacccccaga aggggttcgc gccgccgaag aacatcgccg
      901 ccacgcccat gaaaccgcgg ccgttggtca tgttctccac gaacatgcgg gagtacccga
      961 gcgagaggtg cgccccggcg atgccgccga gcaccccgga gtagagcatc acctcgaact
     1021 tgcgaaacgt cacgttgatt ccggccgtct ccgccgccag cgggttgagc cccacgctgc
     1081 ggaggcggag cccccacacc gtcttgtaga ggatgaaggc gaggacgaag acgaggacga
     1141 tcccgaccgg ctcgaagagg gagtagtcgt tgaagagggt gtcgaggagc gcgttgccgc
     1201 cgaagtcgaa attcaggcgc ggcatcggga cgatgtcgtt gtggtggaag ctgccggaga
     1261 cgccgaaggt gcgctggagc agaaagcgcg tcatcgcgat ggcaagcatg ttcacgccca
     1321 tgccgacgac gaagatgtcc gccttcgcct tgagatgcgc gaaggccatg aaggccgcga
     1381 cgacgacgcc ggtaacgatg gcggcaagaa ggcccagcac ccagcttccg gcgaagtagc
     1441 tggtggtcac ggccgcgaag gcggcgaaga tcatgatgcc ctcgataccg atgttcaata
     1501 tgttcgcctg ctgcgtcagg acgcacgcca gcgtggcgta cagaatcggc gtagcggcgc
     1561 ggatggtcga gcggacgagg gtcgcgttga agacgaggga gagtatctcg ttcagtccgt
     1621 ccattacgca gcctcctctc cggagctccg ggcgcttctg cgccgcgaat aggcgaagag
     1681 cccctccata gcggcgagca ggatgaagac cgcgataatc gtgtcgatca gggtcttggg
     1741 gactcctgtg aagcgttcca tccccagcgc ccctgtcttg agaacggcca ggaagagcga
     1801 gagtacggga accatgcgga tgtcgttctt cgcgatcagc gaggcgagca tcccgtcaaa
     1861 ggcgattccc ggcgagaagt tgtcgaggaa gtgcccgtgg atgcccatca cctcgatggc
     1921 ccccgcgaga ccgccaatgg ctccggagag aatcatcccc cagatcatcg ccttcttcgg
     1981 aggaatgccg acgtactcgg agaaagaggg attctgcccg acgtggcgca gcttgtaccc
     2041 ccagttggta tgatagagca tccaccagac cagcacgagc acgccgagcg cgacgaagat
     2101 accgatgttt gcccggctgg gacggagaat ccgaagcaga cgcgccgact cttcgatggt
     2161 gggcgttctg gggatgctcg cgcgcgcgga gaaggggttc cctaccaggt aggaggtgaa
     2221 gtagatggcg acgtagttca tcaggatggt gacgcagacc tcgttcacct tccagtacgc
     2281 cttcagcacg ccggggatgg ccccccacgc tccggaagcg accattgcga cggcgaaaca
     2341 gagaggaata tggacaagag gatgaagccc ggtgaaagcg taccccgccc acgccgcggc
     2401 gatggcgccc aggtagagct ctccctcgac gccgacgttg aacacggcaa cctttgcgga
     2461 gaccgcgaag gcaagggccg taagcatcag cgggacgaaa cgttccatga cgccgccgat
     2521 gttgaatttt cccacgaagg ccccgcggaa gagctggtag taggcttcga tgggatcgta
     2581 gccgagcagc aggatggccc ccgctcctaa ggcaagggag aggatgatgg tcatcagcgt
     2641 gctgccccag gatgtcttat acatgcgtcc cgcctccagt catcagcagg ccgagcttct
     2701 cctcgtcggc ctccgaggaa tcg
//
LOCUS       DAOTIL010000317         2719 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminobacteriaceae bacterium isolate AMR_MDS_3026
            NODE_44302_length_2719_cov_3.552553, whole genome shotgun sequence.
ACCESSION   DAOTIL010000317 DAOTIL010000000
VERSION     DAOTIL010000317.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868050
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminobacteriaceae bacterium (wastewater metagenome)
  ORGANISM  Aminobacteriaceae bacterium
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae.
REFERENCE   1  (bases 1 to 2719)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2719)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:19:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,224
            CDSs (total)                      :: 3,198
            Genes (coding)                    :: 3,184
            CDSs (with protein)               :: 3,184
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2719
                     /organism="Aminobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_44302_length_2719_cov_3.552553"
                     /isolate="AMR_MDS_3026"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:3034609"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            426..1472
                     /gene="thrC"
                     /locus_tag="P5201_07380"
     CDS             426..1472
                     /gene="thrC"
                     /locus_tag="P5201_07380"
                     /EC_number="4.2.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013047974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004795 - threonine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009088 - threonine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine synthase"
                     /protein_id="HRV98392.1"
                     /translation="MKKGILFSYGKYLPLTEKTPMLSLCEGDTPLVPLRNLSRELGVG
                     LFGKYDGLNPTGSFKDRGMVLAVSKALEEGAKAVICASTGNTSASAAAYAAVAGIPCF
                     VLLPAGKVALGKLAQAMIYGARVLAVRGNFDRALEMAREGAEQDGFAIVNSVNPYRLW
                     GQRSAAWEICDELGAPPDWLVLPVGNAGNISAYCAGFEFYKEQGITRNIPRLVGVQAE
                     GAAPMVNGAPVDAPETVATAIRIGHPVSADKAKRAVEISGGRFLAVSDDEILDAQKLL
                     ASSDGVFAEPASCAPLAGLLKLKREGGLPSGLRIVMVLTGNGLKDPDAATSRVPAPVE
                     IDGTREALMEVLHS"
     gene            1469..>2719
                     /gene="thrB"
                     /locus_tag="P5201_07385"
     CDS             1469..>2719
                     /gene="thrB"
                     /locus_tag="P5201_07385"
                     /EC_number="2.7.1.39"
                     /inference="COORDINATES: protein motif:HMM:TIGR00191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004413 - homoserine kinase activity
                     [Evidence IEA];
                     GO_process: GO:0009088 - threonine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine kinase"
                     /protein_id="HRV98393.1"
                     /translation="MSLLRVKVPASSANLGSGFDTVGVALSLYNFFDVLEILPKGQYE
                     IDVIGEGGGDPSLLEKNGVVESYERACSEWGVEPPGLRLRTMNAIPLKRGLGSSSSAI
                     VGGVAIANELRERPLPKEELLPLMVSMEGHPDNVVPCCLGGMVVSCWDGTDLKYVRMP
                     PLPSDILAVVAVPAVELSTEEARRALPKHVSMGDAVYNVSRSALLAASWATGNWDNLG
                     WAMDDRLHQPFRARLFPGGEAILDEVRRIPQCSGVAISGSGPSVLAFARQEAHRVAEL
                     MCSIFSRFGVRSRFFVLAEDAAGVTVSRNVGCSEIFRPLRKGSVFSMTFGVDSSGRCV
                     VGEVISDRDVAKIAVLGVPDVPGVAARLFSDLAAAGVGAEMIVQSVMRGQMNDIAFII
                     KQSLLGEAIAICRSFASDIGAQGVT"
BASE COUNT          463 a          780 c          952 g          524 t
ORIGIN      
        1 ttatgccacg ctcctttatg aatatctgtc ctccgcgccc cctcttttcc gggtcccctc
       61 ttgacacttt ccggcgacga aggtataaga ggaaaatgtt ttccaatgat atgataaaaa
      121 gacgatgaag ggaagagtac cttctctccg aaggacagag aggaagggcc gaggctggaa
      181 gcccttctcc cgaggagcgc agagaagacc acccctgagt cggcgtcgaa gttcgcgcgg
      241 tttcgcacgc ggagggaaga cgccggggtc cgccccttac agcggtgtcg agttgccggt
      301 atcctcccgg cggatgcgcg aagcagccga gagaggtccg gaaatggagt ggaaccgcga
      361 tgagcctgca tcgtctcctg tattgtggag ggtgcgggct ttttgtatta caggagggat
      421 gttccatgaa gaagggcata ctgttttctt acggaaagta tcttccgctg acggagaaga
      481 cgccgatgct gtcgctgtgc gaaggcgaca cgccgctcgt tccgctgcgg aatttgagcc
      541 gggagctcgg cgtcgggctg ttcgggaagt atgacggcct gaaccccacc ggttcgttca
      601 aggacagggg gatggtgctc gcagtgtcga aggcgctcga ggaaggggcg aaggcggtta
      661 tctgcgcctc caccggcaat acgtcggcct ctgccgcggc ctacgcggcc gtcgcgggca
      721 ttccctgctt cgtgctcctt cccgcgggca aggttgcgct ggggaagctg gcgcaggcga
      781 tgatctacgg agccagagtg ctcgccgtca ggggtaattt cgacagggcg ctcgaaatgg
      841 cgcgggaggg ggcggagcag gacgggttcg ccatcgtgaa ctccgtaaat ccgtaccgtc
      901 tgtgggggca gaggagcgcg gcgtgggaga tctgcgacga actgggcgct ccccccgact
      961 ggcttgtcct tcccgtaggg aacgcgggca acatcagcgc ctactgcgca ggctttgaat
     1021 tctacaagga gcagggtatc acgcggaaca tcccccgcct cgtcggcgtg caggcggaag
     1081 gcgccgcccc gatggtgaac ggcgctccgg tcgatgctcc ggagactgtg gcgacagcga
     1141 tccgcatcgg ccatccggtg agcgcggaca aggcgaaacg cgcagtcgag atctccgggg
     1201 ggcgtttcct cgccgtttcg gacgacgaga ttctcgacgc gcagaagctc cttgcttcct
     1261 cggacggcgt cttcgcagag cccgcttcct gtgcgccgct cgccggactc ctgaaattga
     1321 aaagagaggg cgggctcccc tccggactgc ggatcgtcat ggtgctcacg gggaacggcc
     1381 tgaaggatcc cgacgcggcg acctcccgcg tacccgctcc tgtcgagatc gacgggacgc
     1441 gcgaggcgct catggaggtg ctgcactcgt gagcctgctg cgggtgaagg ttcccgcctc
     1501 cagcgcgaat ctcggctccg ggttcgacac agtaggggtg gccctctccc tgtataactt
     1561 cttcgatgtg cttgaaatac tccccaaggg acagtacgaa atcgacgtca tcggcgaggg
     1621 cgggggagac ccatcgctgc tggagaaaaa cggggttgtc gagagctacg agagggcgtg
     1681 cagtgagtgg ggggttgagc cgcccggcct gcggctgcgc acgatgaacg ccatccctct
     1741 gaagcgcggg ctcgggagct cgtcctccgc catcgtgggc ggcgtggcga tagcgaacga
     1801 gctccgggag cggccgcttc cgaaggagga gctgctgccg ctgatggtct ctatggaggg
     1861 gcacccggac aacgtcgtcc cctgctgcct tggggggatg gtggtgagct gctgggacgg
     1921 gacggatctg aagtacgtcc ggatgccgcc gctcccttcc gatattctgg ctgttgtagc
     1981 ggttcccgcg gtcgagctga gcaccgaaga ggcccgccgt gcgcttccga agcatgtttc
     2041 tatgggggac gccgtgtata atgtgagccg ttccgcgctg ttggcggcgt catgggccac
     2101 gggcaactgg gacaacctgg ggtgggccat ggacgaccga ctccatcaac cgttccgggc
     2161 tcggctcttc ccggggggcg aggcgatcct tgacgaggtc cgccgcatcc cgcagtgctc
     2221 cggagtggcg atcagcggtt cggggccgag cgtgctcgca ttcgcccggc aggaggcgca
     2281 cagggttgcg gagctgatgt gctctatctt ctcgcgtttc ggcgtcagat cccgtttctt
     2341 cgtcctcgcg gaggatgcag ccggggtgac cgtgagcaga aatgtcgggt gttccgagat
     2401 tttccgtccg ttgcggaagg ggagtgtgtt ttcaatgaca tttggggttg acagttccgg
     2461 acgctgcgtg gtcggcgagg tgatctccga ccgggatgtt gcgaagatag ccgttctcgg
     2521 cgtgccggac gtccccggcg tcgcggcgcg tctcttctcc gatcttgccg cggcgggagt
     2581 gggagcggag atgatcgtgc agagcgttat gagggggcag atgaacgaca tcgccttcat
     2641 catcaaacag agtctcctgg gcgaggccat cgcgatctgc aggtctttcg cctccgatat
     2701 cggggcgcag ggcgtgacc
//
LOCUS       DAOTIL010000318         2715 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminobacteriaceae bacterium isolate AMR_MDS_3026
            NODE_44384_length_2715_cov_3.153008, whole genome shotgun sequence.
ACCESSION   DAOTIL010000318 DAOTIL010000000
VERSION     DAOTIL010000318.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868050
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminobacteriaceae bacterium (wastewater metagenome)
  ORGANISM  Aminobacteriaceae bacterium
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae.
REFERENCE   1  (bases 1 to 2715)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2715)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:19:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,224
            CDSs (total)                      :: 3,198
            Genes (coding)                    :: 3,184
            CDSs (with protein)               :: 3,184
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2715
                     /organism="Aminobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_44384_length_2715_cov_3.153008"
                     /isolate="AMR_MDS_3026"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:3034609"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2370)
                     /locus_tag="P5201_07390"
     CDS             complement(<1..2370)
                     /locus_tag="P5201_07390"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_process: GO:0009966 - regulation of signal transduction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HRV98394.1"
                     /translation="MLPPTKSVSIKDIILLAFVMLTLITGGISGFIVFSGWLSSAEEN
                     AGRMADEISDVIFSRVNAHFNIPLHINAAYREQLEKGVVDMNNPLQRERFLASVMRAH
                     SGDVIYSFGYATAEGEYYAVRWNERNELEFVRNSAETGGRSFYYAVTDDLTAGEFVMD
                     AGAFDPRTRNWYVAARDAEGPVFSPIYKHFVMPDLTVSASCPVRAADGVLKGVLGVHT
                     TLSKINGYLEETIRNKGGVAAIVERESGAYVANSMGLPNFSLLPDGRVHRLSAKEGGD
                     AVLKTAYEEYLRKGAHGMKIEDGAGAFFVRMEEYRLNGLDLLISSAIPERIFTAGVFS
                     SMRLALALVILVLLAAITLYSALASAFMRPMEALIDAAEQFSRGDLEKRATVVRDDEI
                     GRLSRTFNAMADNIYLLVGTLESKVKERTAKLEKANAALNESEDRLRLILDSTAEAIY
                     GIDAEGRCTFANASCLRMLGYGREEELLGRDMHALIHYSQRDGRPIRQDECRIFQVLG
                     MGESAFADDEVFWRADGDSFDVEYSAYPQYKDGKLVGAVVAFMDNTERRKNRAHIDFL
                     TYHDALTGLYNRMYFERALKSLDAEEHLPVTIIFGDVNGLKLTNDVFGHAAGDTLLKR
                     SAEILKKVCRESDVVARVGGDEFAVLLPRTGPEAVKKVIERIRNEFGKERILAIKASI
                     SMGYAVKTGPDEQLERTVKDAEDAMYKEKTLDRKSVAAEMVRTILETLYARSPREQKH
                     SLAVAELCADAGRALGLPEPETRRLREMGLLHDIGKIAVDEGLLNKNGTF"
     gene            complement(2517..>2715)
                     /locus_tag="P5201_07395"
     CDS             complement(2517..>2715)
                     /locus_tag="P5201_07395"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRV98395.1"
                     /translation="DDRFDTSRKEAMYCTEVIWRAYKAAGIDLARGNWELARNPIVQG
                     RVLLPYKLSLSPHLEEAFVLE"
BASE COUNT          482 a          865 c          773 g          595 t
ORIGIN      
        1 gaacgttcca tttttgttca acagaccttc gtccacggcg attttgccga tgtcgtgcag
       61 cagccccatc tcacgcaggc ggcgcgtctc cggctcagga aggccgagcg cgcgccccgc
      121 gtcggcgcac agctccgcga cggcgagaga gtgtttttgc tcccgggggc tcctggcata
      181 gagcgtctcc agaatcgtgc gcaccatctc cgccgccacg ctcttccggt cgagcgtctt
      241 ctccttgtac attgcgtcct cggcgtcctt cacagtgcgt tccaactgct cgtccgggcc
      301 tgtcttcacg gcatacccca tggagatgct cgcctttatc gcaaggatcc gctcttttcc
      361 gaactcgttc cggatccttt cgataacctt cttcaccgcc tccggcccgg ttcgaggcag
      421 cagaacggca aattcgtcgc ctcctaccct ggcgacgacg tcgctctccc tgcacacttt
      481 cttcagtatc tccgccgacc gtttcaggag cgtatctccc gcggcgtgcc cgaagacgtc
      541 gttcgtcagc ttgagaccgt tgacgtcccc gaagatgatc gtgacaggaa gatgctcctc
      601 ggcgtcaagg cttttcaggg ctctctcgaa gtacatcctg ttgtacaggc cggtgagcgc
      661 gtcgtggtag gtgaggaagt cgatgtgcgc cctgtttttc ctgcgctcgg tattgtccat
      721 gaaggcgacg accgcgccga caagtttccc gtctttgtac tggggatagg ccgagtactc
      781 cacgtcgaat gagtctccgt cggcccgcca gaagacttcg tcgtcggcga aggcgctctc
      841 gcccatcccg agcacctgaa aaattctgca ctcgtcctgg cggatcggcc tgccgtctct
      901 ctgcgagtag tggatgagag cgtgcatatc cctgccgagc agctcttcct cgcgcccgta
      961 ccccagcatc ctcagacagc tcgcgttggc gaacgtgcac cgtccctccg cgtcgatgcc
     1021 gtagatagcc tccgccgtgg agtcgaggat caggcggaga cggtcttcgc tttcgttcag
     1081 cgccgcgttc gctttctcca gcttcgcggt gcgctccttc accttcgatt ccagcgtccc
     1141 gacgagaagg tagatgttgt cggccattgc gttgaacgtt cgcgaaaggc ggccgatctc
     1201 gtcgtcgcgc acaacggtcg ccctcttttc gagatcgcct cttgagaatt gctcggcggc
     1261 gtcgatcagc gcctccatgg ggcgcatgaa ggcgcttgca agcgcgctgt aaagcgtgat
     1321 cgcggccagg agtaccagaa tgaccagcgc aagcgccagc cgcatgctgc tgaagacgcc
     1381 cgcggtaaag atccgttccg gtatggcgga agaaatgaga aggtccagcc cgttcagacg
     1441 gtattcctcc atacggacaa agaacgcgcc cgcgccgtcc tcgattttca tgccgtgcgc
     1501 ccccttgcga agatactcct cgtaggcggt cttcagtacg gcgtccccgc cttctttcgc
     1561 agagagtctg tgcacgcgcc catccgggag gagagaaaag ttcggcagtc ccatggagtt
     1621 cgccacatac gctccggact ctctttcgac gatggcggcg actccgccct tgttccgtat
     1681 cgtctcctca aggtagccgt tgatcttcga gagagtggtg tgcacgccga gaacgccctt
     1741 caagactccg tcggcggcgc ggaccgggca ggaggcggac accgtcaggt cgggcatgac
     1801 aaagtgcttg tagatcggcg agaagaccgg tccctccgca tccctcgcgg caacatacca
     1861 gtttctcgtg cgggggtcga aagcgcccgc gtccatcacg aactctccgg cggtcaggtc
     1921 gtcggttacc gcataataaa aagagcgccc gccggtctcc gcgctgttcc gtacgaattc
     1981 cagctcgttc cgttcattcc accggacggc gtagtattcc ccctccgctg tggcgtagcc
     2041 gaagctgtag ataacgtcgc ccgaatgcgc gcgcatcacg gaagccagaa agcgctcccg
     2101 ttgcagcgga ttgttcatgt cgacgacgcc tttttccagc tgttcgcggt aggcggcgtt
     2161 gatatgcagg ggaatgttga aatgcgcgtt caccctgctg aagatgacgt cgcttatctc
     2221 gtcggccatc cggccggcgt tctcctcggc ggacgagagc catccggaga agacgatgaa
     2281 gcccgaaatc ccgccggtga tcagcgtcag catcacgaac gccaaaagga tgatgtcttt
     2341 tattgagacg ctcttcgtcg gcggaagcat gggctcacct cgatcattcc gggctcttca
     2401 ttatgaaggc agttgtaatt atacagacaa aattcccttt tattcaaccg ctcgttccgt
     2461 cgtgaccgtg acaccgtgta aatgggtatt cttgcagttc gcaaaaggac gaggcgctac
     2521 tcgagcacga acgcttcctc cagatgcggg ctcaggctca gtttgtaggg aaggagcact
     2581 ctcccctgga cgatggggtt gcgcgcgagc tcccagtttc cccgcgccag gtcgattccc
     2641 gccgccttgt aggcgcgcca gatcacctcg gtgcagtaca tggcttcttt tctcgacgta
     2701 tcgaaccggt cgtcg
//
LOCUS       DAOTIL010000319         2714 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminobacteriaceae bacterium isolate AMR_MDS_3026
            NODE_44399_length_2714_cov_3.643475, whole genome shotgun sequence.
ACCESSION   DAOTIL010000319 DAOTIL010000000
VERSION     DAOTIL010000319.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868050
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminobacteriaceae bacterium (wastewater metagenome)
  ORGANISM  Aminobacteriaceae bacterium
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae.
REFERENCE   1  (bases 1 to 2714)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2714)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:19:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,224
            CDSs (total)                      :: 3,198
            Genes (coding)                    :: 3,184
            CDSs (with protein)               :: 3,184
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2714
                     /organism="Aminobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_44399_length_2714_cov_3.643475"
                     /isolate="AMR_MDS_3026"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:3034609"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..528)
                     /locus_tag="P5201_07400"
     CDS             complement(<1..528)
                     /locus_tag="P5201_07400"
                     /EC_number="4.2.1.126"
                     /inference="COORDINATES: protein motif:HMM:NF003915.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramic acid 6-phosphate etherase"
                     /protein_id="HRV98396.1"
                     /translation="MIELKHLTTEQRNEKTLDLDSMPLDEALRVMNAEDAKVAPAVGE
                     QLPAIAKAVEAVVRSFESGGRLLYLGAGTSGRLGILDATECVPTFGTPPEMVVGRIAG
                     GMGALVRSVEGAEDSLTMGVGDLKELKADSRDTVVGIAASGRTPYVIAALEYAKSVGC
                     ATVGVTCNRNSAVGAA"
     gene            complement(594..2582)
                     /locus_tag="P5201_07405"
     CDS             complement(594..2582)
                     /locus_tag="P5201_07405"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HRV98397.1"
                     /translation="MTDETYRAMPLPFSSSSGCTEEALRESEENFRTFFEAIDELASV
                     ISLDGNILFTNGAFRRKTGYAEDEIVGMSALDLELPENREKGAEVFAAMLRGELKSCS
                     MPMRAKDGSHVLVETRLWFGRWNGAPCVYTLSKDVGPEQEAQQRFEAVFRNNPAMMVL
                     ASLPDQRCVDANTTFLRTLGYEKEDVVGKQAREIGLFIDPEQQREIGEMMRNDGRITD
                     YEVHAKDRNGDVFHGLMSADRISIHGRPYLLTVTVDITVRKRAEEALLVAKEQAEWLG
                     RQAEAANRAKSAFIANTSHEIRTPLNGVVGFLDLLARTRLDDVQREYVEGIRASAGSL
                     LDVINDVLDISKIEADKVELRPAPTDIRELAGQAADTVLGTARTKGLHLTVNVAPEVP
                     GAFFLDQSRLKQVLINLLGNAVKFTHEGGVSFSIRCEEAEEDRCLLRFSVEDTGIGIR
                     PESMERLFEPFFQADVSNTRKYGGTGLGLSICSALLQMMGSELRVESVPGEGSRFFFD
                     LRAERVPVLETAEEERARIVPEEPFMPATRPTILIIEDEELNRRLLRLLLSKLLDADI
                     VEAEGGEEGVERFRERAPDLIFLDLQMPGRDGHWTARTLRELEGAGVHTPIVAFTADV
                     QPATREACFASGMDDYICKPADLYTLREVLERHLKTKT"
BASE COUNT          423 a          951 c          826 g          514 t
ORIGIN      
        1 cgcagcgccc actgcggagt ttctattgca tgtgacgccc accgtcgcgc agccgacgct
       61 cttcgcgtac tccagcgcgg cgatcacgta cggcgtgcgt ccgctcgccg ctatccccac
      121 cacggtgtcc ctggagtcgg ccttgagttc cttcaggtcg cccacaccca tggtcaggct
      181 gtcctccgcg ccctccacgg agcgcaccag cgctcccatg ccgcccgcga tacgccccac
      241 gaccatctcc ggcggcgtgc cgaaggtggg aacgcactcc gtcgcgtcga ggattcccag
      301 gcgtccgctg gtccccgcgc ccaggtagag cagtcgcccc ccgctctcga aagagcggac
      361 cacagcctcc accgccttcg caatggcggg gagctgttcc cccactgcgg gggcgacttt
      421 cgcgtcctcc gcgttcatca cccggagggc ctcgtcgagg ggcatgctgt ccaggtcgag
      481 cgtcttttcg ttgcgctgtt ccgtcgtcag gtgcttcagt tcgatcatgc gcgcttcttc
      541 ctttcgttgt gtgcgttgtc gcggcagtct ccggcggggc gccggacgtc ccgctacgtt
      601 ttcgtcttca gatgccgctc cagcacctcg cggagcgtgt agaggtcggc gggtttgcag
      661 atgtagtcgt ccatgccgct cgcgaaacag gcctctctcg tcgcgggctg cacgtcggct
      721 gtgaaggcga cgatcggcgt atgcaccccc gccccctcca gctcccgcag cgtccgcgcc
      781 gtccagtggc cgtcacgtcc gggcatctgg agatcaagga agatcaggtc gggagcgcgc
      841 tcgcgaaagc gttcgacacc ctcttcgccc ccctccgcct cgacgatgtc ggcgtcgagc
      901 agcttggaga gcagcaggcg caacagcctc ctgttcagct cctcgtcctc tatgatcaat
      961 atggtcggtc tcgtcgccgg catgaagggc tcctccggaa cgatccgcgc tctctcttcc
     1021 tccgccgtct ccaaaaccgg gacgcgctcc gcccgcaggt cgaagaaaaa gcggctcccc
     1081 tcgcccggga cgctctcgac cctgagttcg ctccccatca tctggagaag cgcgctgcag
     1141 atggagaggc cgagtccggt gccgccgtac tttcgcgtgt tcgagacgtc ggcctggaag
     1201 aagggctcga agaggcgctc catggattcc ggacgaatgc cgatcccggt gtcctcgacg
     1261 gagaagcgga gaaggcatcg gtcctcttca gcctcctcgc agcggatcga gaacgacacg
     1321 cccccctcat gagtgaactt gacggcgttg cccagcagat tgatcagcac ctgcttcagg
     1381 cgcgactggt cgaggaagaa cgcccccggg acctccgggg cgacgttcac cgtaagatgc
     1441 agccccttcg tccgcgccgt gccgaggact gtatccgccg cttgccccgc aagctcccgg
     1501 atatccgtcg gcgccggccg aagctcgacc ttgtccgcct cgatcttcga gatgtcgagc
     1561 acgtcgttga tcacgtcgag gagagagccc gccgacgcgc gtatcccctc gacgtactcc
     1621 cgctgcacgt cgtcgagacg agttcgcgcc aggaggtcga ggaagccgac gacgccgttg
     1681 agcggggtgc ggatctcgtg gctggtgttc gcgatgaagg cgctcttcgc cctgttcgcc
     1741 gcctccgcct gccgcccaag ccactcggcc tgctccttgg ctaccaggag cgcctcctcc
     1801 gcgcgcttgc ggactgtgat gtccaccgtc accgtcagaa gataggggcg tccgtggata
     1861 gaaatgcggt cggcggacat gaggccgtgg aagacgtcgc cgttccggtc cttcgcgtgc
     1921 acctcgtagt cggtgatcct gccgtcgttt cgcatcatct cgccgatctc gcgctgctgt
     1981 tccgggtcga tgaaaagacc gatctccctg gcctgtttgc cgacgacgtc ctccttctcg
     2041 tagccgagcg tccgcaaaaa cgtggtgttc gcgtcaacgc accgctggtc gggcagcgag
     2101 gcgagcacca tcatggcggg gttgttccgg aagacggcct cgaagcgctg ctgcgcctcc
     2161 tgctccggtc caacgtcctt gctgagggtg tagacgcacg gcgcgccgtt ccaccggccg
     2221 aaccagagcc gcgtttcgac gaggacgtgc gagccgtcct tcgcccgcat gggcatagaa
     2281 cagctcttca gctccccgcg cagcatggcc gcgaagacct cggcgccctt ctcccggttc
     2341 tcggggagct ccagatccag cgcgctcatc cctacaatct catcctcggc gtagcccgtc
     2401 tttcgccgga atgcgccgtt ggtgaacagg atgttgccgt cgagcgagat gacgctcgcc
     2461 agctcgtcga tggcctcgaa gaacgtgcgg aagttctcct cgctctcccg gagggcctcc
     2521 tccgtacagc cggaagatga ggagaaggga aggggcatcg cccggtacgt ttcatccgtc
     2581 atttcggaac ctccatgagc ggtgcgctcc attcgatgcg gaacgaaggg ccgaagaacg
     2641 ccgaaaatgc cctctccgcc cctcttcgca tactggaaag acggcccgaa ggccgcctgc
     2701 ggtcattttt tata
//
LOCUS       DAOTIL010000320         2708 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminobacteriaceae bacterium isolate AMR_MDS_3026
            NODE_44545_length_2708_cov_2.678100, whole genome shotgun sequence.
ACCESSION   DAOTIL010000320 DAOTIL010000000
VERSION     DAOTIL010000320.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868050
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminobacteriaceae bacterium (wastewater metagenome)
  ORGANISM  Aminobacteriaceae bacterium
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae.
REFERENCE   1  (bases 1 to 2708)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2708)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:19:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,224
            CDSs (total)                      :: 3,198
            Genes (coding)                    :: 3,184
            CDSs (with protein)               :: 3,184
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2708
                     /organism="Aminobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_44545_length_2708_cov_2.678100"
                     /isolate="AMR_MDS_3026"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:3034609"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..432)
                     /locus_tag="P5201_07410"
     CDS             complement(<1..432)
                     /locus_tag="P5201_07410"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRV98398.1"
                     /translation="MFRKTVARSFWVAALIAVVCAGAAVAMDVEQAADKVYLAAVEGT
                     PFPVLSAEYADMTEAGSYAVQKAYSLKRLEGKRPAGFKAGLTTEATMKRFGASTPFAA
                     PLFPEGAMDGSAESVVVDRASFGVLMLEAEIHSDSYRFNDRT"
     gene            613..1005
                     /locus_tag="P5201_07415"
     CDS             613..1005
                     /locus_tag="P5201_07415"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HRV98399.1"
                     /translation="MKRILVVDDSAVSRKLALFVLKNLGCEARGVPSAAEAFEALRNE
                     VYDAIFMDLHMPEMNGCEASMFIRRSGAGEANRSLPIFAITGSDLPEERESCVSAGMT
                     DFLAKPFSGEHAEALLRKHCGKTAHDGQ"
     gene            1064..1786
                     /locus_tag="P5201_07420"
     CDS             1064..1786
                     /locus_tag="P5201_07420"
                     /inference="COORDINATES: protein motif:HMM:NF023540.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative metalloprotease CJM1_0395 family
                     protein"
                     /protein_id="HRV98400.1"
                     /translation="MSGISAVPPVNSIKTVVPVANRTRREGLQRAEREVMRQELAVRN
                     QGDVTSTVYHYEIGPDGRRYITGATVTYKEKENELSSKSAKDKSSASSANTGPASSAE
                     NGRVELTDESRAKLKAIERDVIAHEAAHKAVGGQYAGPVSYTYATAPDGTRYIVGGEV
                     SISAPEGKTPEETIQIMEQVKRAALAAGSPSPQDIQVAAAASAAQMRARAEMAKQEAE
                     KAYSGEKGAQDFSRWGEFAMSA"
     gene            complement(1872..>2708)
                     /locus_tag="P5201_07425"
     CDS             complement(1872..>2708)
                     /locus_tag="P5201_07425"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HRV98401.1"
                     /translation="INMFTGLARPDEGTIRIGGVDCTANPRAAQRLIGVVPDESNLYP
                     ELTGFENLSFCASLYGMRKAERRKRATELLDMFGLSKAGFRKFGGYSKGMKRKLTIAA
                     GIIHRPRVLFLDEPTTGIDVASARQVRRLIADLHREGTTIFLTTHFIEEAERLCERIA
                     FLVQGRIERIDTVENLLQPLREKHVVHIACAVVLDDVRRALSENFPEASFACMEAGRI
                     RVESEEPIAVGKLVRFLEENEVEVYEARKTTPSLEEVFVRITGIEAEEMRAERAGPGM
                     AR"
BASE COUNT          585 a          822 c          763 g          538 t
ORIGIN      
        1 ggtgcggtcg ttgaagcgat agctgtcgga gtggatctcc gcttccagca tcagaacgcc
       61 gaaagaagcg cggtcgacga cgacgctctc cgcgctcccg tccatcgcac cctcggggaa
      121 gagcggcgcg gcgaagggcg tggaggcccc aaaacgcttc atcgtcgcct cggtggtgag
      181 cccggccttg aaccccgccg gacgcttccc ttccaggcgt ttcaaagaat atgccttctg
      241 tacggcgtac gagccggcct ccgtcatatc ggcgtactcc gcggagagga cggggaaagg
      301 agtcccttcc acggcggcaa gatagacttt gtccgccgcc tgctccacgt ccatcgcaac
      361 cgccgcgccc gcgcacacaa ccgcgatcag cgccgctacc caaaaactcc gtgccactgt
      421 tttccgaaac acattcaaca cctctttcac aaaaaagtcg gatcttcaga ctgcgcgcag
      481 cctttcaatc tgtggtgtat tatactcttc cttttttcgg ttttcccggc atactcttct
      541 caagtgaaga gatgctacaa tagtaccgac gctacgatac gccttctcgg cggggaagtt
      601 gggggaaaag agttgaagcg aattcttgtt gtggacgaca gcgctgtgag ccggaaactc
      661 gctctcttcg tccttaaaaa tcttgggtgc gaggcgcgag gagtaccctc ggccgccgag
      721 gccttcgaag cgctgcgaaa tgaagtctac gacgcaatct ttatggatct gcacatgccg
      781 gaaatgaacg gctgcgaagc gtcgatgttc atccgccggt caggcgccgg agaagccaac
      841 cgctctctgc cgattttcgc gattactgga agcgatctgc ccgaggaaag agaaagctgc
      901 gtatcggcgg gtatgacaga tttcctggca aaaccgttct ccggagaaca tgcggaagcg
      961 ctgttgagaa agcattgcgg gaagactgca cacgatggac aataatccgg gatgtgctat
     1021 aattggttga atagatagtt tttaaaggga ggaggcaatg gatatgtccg gtatttcggc
     1081 tgtacctcct gtaaacagca tcaagacagt cgttcccgta gccaacagaa cccggcgtga
     1141 aggtttgcag cgcgccgaac gagaagtcat gcgccaggag ctggccgtga ggaaccaggg
     1201 cgacgtcacc tccaccgtct accactacga aataggtccc gacggaaggc gctacattac
     1261 cggagctacg gtaacctaca aagaaaaaga aaacgagctc tcctcaaagt ctgcgaagga
     1321 caaatcttca gcgagctctg caaatacagg acccgcctcc tccgcagaaa acggccgcgt
     1381 cgagctcact gacgagagcc gggccaagtt gaaggcgatc gaacgcgacg tcatcgcgca
     1441 cgaagccgcc cacaaggcag tcggcggaca gtatgccgga ccggtttcct atacgtacgc
     1501 cacagctccg gacggcacgc gctacatcgt cggcggagaa gtctccatct ccgctcccga
     1561 agggaagacc ccggaagaga ccatccagat aatggagcag gtgaagcggg cggcgctggc
     1621 ggcaggttct ccatctccgc aggatataca ggtcgcggcc gcagcttctg cggcccagat
     1681 gcgcgcacgc gccgagatgg caaagcaaga ggctgaaaaa gcctactccg gcgaaaaagg
     1741 cgcacaggac ttctccaggt ggggcgagtt cgcgatgtcg gcgtaatctg cttcatcaac
     1801 atcttcagac gataaaaaga cgtcctgttc gcggacgtct tttttcatgg tcatttttgc
     1861 ctcccggtcg tttatcgcgc cattcccggt ccggctctct ccgcgcgcat ctcctcggcc
     1921 tcgataccgg tgatgcgaac aaagacctcc tccagggagg gagttgtttt cctcgcctcg
     1981 tacacctcga cctcattctc ttcaaggaag cggacaagct tgccgaccgc tatcggctcc
     2041 tcgctttcga cacggattct cccagcctcc atgcaggcaa acgacgcctc cgggaagttt
     2101 tcggagaggg ctcgacgcac atcgtccagc acgaccgcgc atgctatgtg cacgacgtgc
     2161 ttctcccgga gcggctgcaa cagattctcg accgtgtcga tccgctcgat cctcccctgc
     2221 acaagaaagg cgattctttc gcacaggcgc tccgcctcct caatgaagtg cgtcgtcaga
     2281 aagatggtcg tcccctcccg atgaagatcg gcgatgaggc gccgtacctg cctcgcgctc
     2341 gcgacgtcga ttcctgtggt tggctcgtcc aggaagagga cgcgcggacg gtggatgatc
     2401 ccggcggcta tggtcagctt ccgcttcatc cccttggagt agccgccgaa cttcctgaat
     2461 ccggctttgc tcagtccgaa catgtcgaga agctccgtag cccgtttcct ccgttcggct
     2521 ttgcgcattc cgtagagcga agcgcagaac gagaggttct cgaatcctgt cagctccggg
     2581 taaagattgc tctcgtccgg tacaacgccg atcagccgct gtgccgcccg cggatttgcg
     2641 gtgcagtcca cgccgccaat gcggattgtc ccctcgtcgg ggcgtgccag ccctgtaaac
     2701 atgttgat
//
LOCUS       DAOTIL010000321         2708 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminobacteriaceae bacterium isolate AMR_MDS_3026
            NODE_44546_length_2708_cov_2.440256, whole genome shotgun sequence.
ACCESSION   DAOTIL010000321 DAOTIL010000000
VERSION     DAOTIL010000321.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868050
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminobacteriaceae bacterium (wastewater metagenome)
  ORGANISM  Aminobacteriaceae bacterium
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae.
REFERENCE   1  (bases 1 to 2708)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2708)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:19:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,224
            CDSs (total)                      :: 3,198
            Genes (coding)                    :: 3,184
            CDSs (with protein)               :: 3,184
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2708
                     /organism="Aminobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_44546_length_2708_cov_2.440256"
                     /isolate="AMR_MDS_3026"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:3034609"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..644
                     /locus_tag="P5201_07430"
     CDS             <1..644
                     /locus_tag="P5201_07430"
                     /inference="COORDINATES: protein motif:HMM:NF015817.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DbpA RNA binding domain-containing protein"
                     /protein_id="HRV98402.1"
                     /translation="DARTLKSLLRGHSLSIEWLSVPDSQEIRLLERGRLDRELAECEE
                     EPQETYLEWAREILGRSEAECSIARLLERFFAQRSSGFALRKELDREMEAEERRRLAK
                     RDGAPGTRSRGPREKDSGGMTRLRLERGRADGLEVGALLGVLCRTLGVDRREIGNIRL
                     RDRYAEVELGARAARQLERHRRELEREGYLSPEGAQGQRKARTPGERDRSHNR"
     gene            935..2614
                     /locus_tag="P5201_07435"
     CDS             935..2614
                     /locus_tag="P5201_07435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006584113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl-CoA mutase family protein"
                     /protein_id="HRV98403.1"
                     /translation="MSESKSAGYAEGQSAYEKTVEKFLQKAPERKQAFRTGGGLPLKR
                     IYGPEDLEGMDYVQDLGFPGMYPYTRGVQPTMYRGRFWTMRQYAGFATAEESNARYRY
                     LLSQGTTGLSVAFDLPTQIGYDSDHAMAQGEVGKVGVAIDSLADMETLFDQIPLDKVS
                     TSMTINAPASVLLALYIAVGEKQGVSMEKLSGTIQNDILKEYIARGTYIFPPKPSMRL
                     ITDIFDFCSTHVPQWNTISISGYHIREAGSTAVQEVAFTLADGIAYVEAAVKKGQDPN
                     VFGRRLSFFFNAHNDFLEEVAKFRAARRLWAKIMKERFGVTDRTAQTLRFHTQTAGST
                     LTAQQPENNIVRVAVQAMAAVLGGTQSLHTNSYDEALALPTEKSVGIALKTQQIVAYE
                     SGVTETVDPLAGSYVVESLTNEIERQSAEYIGKIDGMGGMLAAIEKGYVQQQIQDAAY
                     TTQKAIESNEQIVVGVNKFQVDEGAVERSLLKVDSSVGANQVKKLQSMKAGRDNLKVQ
                     DCLESIRAASREEGTNLMPKIVEAVKAYVTEGEICGVLREEFGEYRENIVL"
BASE COUNT          586 a          681 c         1000 g          441 t
ORIGIN      
        1 gggacgcccg gacgctcaag agcctcctgc gcgggcactc gctgagcatc gagtggcttt
       61 ccgtgccgga ttcccaggaa atcaggctac tcgaaagggg gcgcctcgac cgggaactgg
      121 ccgagtgcga ggaagagccc caggaaacct atctcgaatg ggcaagggaa atcctgggac
      181 gaagcgaagc cgagtgttcc atcgcccgcc ttctcgagcg atttttcgcc cagcggtcct
      241 cgggtttcgc cctcaggaag gaactggaca gggaaatgga ggcggaagaa cgaaggcggc
      301 ttgcgaaaag ggatggcgcc cccggtaccc gctcacgcgg gccgcgggag aaggactccg
      361 gcgggatgac ccgcctgagg ctggaaaggg gtagggctga cggcctcgaa gtcggggccc
      421 ttctcggcgt cctttgccgt acgcttggcg tcgaccgcag ggagataggg aatatccggc
      481 ttcgcgaccg gtatgccgag gtcgagctcg gtgcccgggc agcccggcag ctcgagcgcc
      541 accggaggga actggagagg gaagggtacc tttcccctga gggggcccag ggacagcgga
      601 aggcaaggac cccgggtgag agggaccgtt cccacaatag ataggaagat tgcgcaacgg
      661 agggcgggac caacccgccc tccgtttgtt tggatacaaa atacgatcct ctcttcttgc
      721 gtcccagccc cccggtgaca tgggtgaaaa atgacgtaaa tgcaggcagc gagcggtatt
      781 ggcctatttt gtttataatt tttgcacaat tccacataaa tggttgcatc gtggttcagt
      841 ttgttcgata atcgggaggt ttatgcgctt cgtatttttt tgtgaaaaaa caatcattgg
      901 tatcggcaac gatagcaggg aggggacgga gcacatgtcg gagagcaagt ctgcgggata
      961 cgcggaggga cagtccgcgt acgagaagac ggtggagaag tttctccaga aggcgccgga
     1021 gaggaagcag gcgttccgga cgggcggcgg gttgccgctg aagcggattt acggcccgga
     1081 ggacctggag gggatggatt acgtccagga cctgggattc ccggggatgt acccgtacac
     1141 gcggggagtg cagccgacga tgtaccgggg acgtttctgg acgatgcggc agtacgcggg
     1201 attcgcgacg gcggaggagt ctaacgcgcg gtaccgttac ctgctgtcgc aggggacgac
     1261 gggcctgtcg gtggcgttcg acctgccgac gcagatcggt tacgactcgg accacgccat
     1321 ggcccaggga gaagtgggga aggtgggcgt ggcgatcgac agcctggcgg acatggagac
     1381 gctgttcgac cagatcccgc tggacaaggt gtcgacgtcg atgacgatca acgccccggc
     1441 gagcgtgctt ttggcgctgt acatcgcggt gggcgagaag cagggcgttt cgatggagaa
     1501 gctttcgggg acgatccaga acgacatttt gaaggaatac atcgcccgtg ggacgtacat
     1561 tttcccgccg aagccgtcga tgcggctgat cacggacatc ttcgatttct gcagcaccca
     1621 cgtgccgcag tggaacacga tctcgatctc gggctaccac atccgcgagg cgggaagcac
     1681 ggcggtgcag gaagtggcgt tcacgctggc ggacggtatc gcgtacgtgg aggcggcggt
     1741 gaagaagggt caggacccga acgtgttcgg caggcggctg agcttcttct tcaacgcgca
     1801 caacgacttt ttggaggaag tggcgaagtt ccgggcggct cgtcgcctgt gggcgaagat
     1861 catgaaggag cggttcggcg tgacggaccg tacggcgcag acgctccggt tccacacgca
     1921 gacggcggga agcacgctga cggcgcagca gccggagaac aacatcgtgc gagtggcggt
     1981 gcaggcgatg gcggcggtgc tgggagggac acagagcctc cacacgaaca gctacgacga
     2041 ggcgctggcg ctgccgacgg agaagagcgt gggaatcgcg ctgaagacgc agcagatcgt
     2101 ggcgtacgaa tcgggcgtga cggagacggt ggatccgctg gcgggaagct acgtggtgga
     2161 gtctctgacg aacgagatcg agaggcagtc ggcggagtac atcgggaaga tcgacgggat
     2221 gggcggaatg ctggccgcga tcgagaaggg gtacgtgcag cagcagatcc aggacgcggc
     2281 gtacacgacg cagaaggcca tcgagtcgaa cgagcagatt gtggtgggcg tgaacaagtt
     2341 ccaggtggac gagggagcgg tggagcgatc gctgctgaag gtggactcgt cggtgggagc
     2401 caaccaggtg aagaagctgc agtcgatgaa ggcaggtcgt gacaacctga aggtgcagga
     2461 ctgcctggaa tcgatccgcg cggcgtcgag ggaagaggga acgaacctga tgccgaagat
     2521 cgtggaagcg gtgaaggcgt acgtgacgga aggcgagatc tgcggcgtgc ttcgcgagga
     2581 gttcggcgag taccgggaaa acatcgtcct ctagggatag gaggagacga aggaagccat
     2641 gagtgagcgg aaagtgcgag tgatcgtggc aaaacccggt cttgacgggc acgaccgggg
     2701 tgcgaaag
//
LOCUS       DAOTIL010000322         2707 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminobacteriaceae bacterium isolate AMR_MDS_3026
            NODE_44561_length_2707_cov_3.497360, whole genome shotgun sequence.
ACCESSION   DAOTIL010000322 DAOTIL010000000
VERSION     DAOTIL010000322.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868050
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminobacteriaceae bacterium (wastewater metagenome)
  ORGANISM  Aminobacteriaceae bacterium
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae.
REFERENCE   1  (bases 1 to 2707)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2707)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:19:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,224
            CDSs (total)                      :: 3,198
            Genes (coding)                    :: 3,184
            CDSs (with protein)               :: 3,184
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2707
                     /organism="Aminobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_44561_length_2707_cov_3.497360"
                     /isolate="AMR_MDS_3026"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:3034609"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1146)
                     /locus_tag="P5201_07440"
     CDS             complement(<1..1146)
                     /locus_tag="P5201_07440"
                     /EC_number="4.2.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014163864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine synthase"
                     /protein_id="HRV98404.1"
                     /translation="MAQPVELKCVLCGASYAPGEVEYTCPVCGLDGTLDVLYDYESAA
                     RTMTKGALAGRERSLWRYEEILPIVDKGSIPHLAVGWTPLYECPAVAEEYGVREFMVK
                     DDGRNPTGSLKDRASAVGVARAIDINQRTIACASTGNAASSLSGFAAVGGLRSFIFVP
                     EKAPVAKVTQLLVYGATVVLVKGDYADVFNLATAAINKYGWYNRNCAINPYLVEGKKT
                     CALEIAEQTGWDLPDRVFISVGDGCCIGGLYKGFSDLLRMGLISRMPKIVGVQAQGAK
                     PIYDAFKSKAERVAFGPADTVADSISVGAPRNWAKALRAVRESEGDMVAVSDEEILSA
                     IPELARKTGVFGEPAGAAAFAGFRKMAKEGLLGRGERIAVVVTGNGLK"
     gene            complement(1130..2467)
                     /locus_tag="P5201_07445"
     CDS             complement(1130..2467)
                     /locus_tag="P5201_07445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005660106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HRV98405.1"
                     /translation="MDEFGVRNLRQAVASVGRDDLIQLCQELLRRPGVSGREGDVARF
                     VAESMRSLGFDSAVIDSYGNVVGEIIFSAEGPRLLLTAQMDHVDAGDVAEWSKYPFGA
                     FIEENRIYGRAASDQKGALASMMLAASLLKRDRSGALRGRLFVAGAVHQETFERVASK
                     AIGDFADPDCVIVGEASGLLLERGQRGRAEMRVDVFGRMAHSSHPKFGVNAADVMVEF
                     LSFLKNRFIPPHDAFLGEGILVLTNLFSSPSYNRESSQREARPDSSAIPERCTAVFDR
                     RLLLGETLEGVVRQFEEIVDEAFRVLPELRAKVSYPVMEDRCYTGAPIRGSHYAPAWT
                     IPSDAPFLRVLSAALEEAGLCPDVSARPGFGTNGCHYAAERLLPTVVYGPSRRELVHT
                     VDEYIEIDALVDSCRGYFAMAERLLASRTLCGQGKKRVSVKEEKRRKVHGAAS"
BASE COUNT          564 a          913 c          728 g          502 t
ORIGIN      
        1 cttcagaccg ttcccggtca cgacgacggc aatccgttcg ccgcgtccga gaagtccttc
       61 tttggccatt ttcctgaacc cggcgaacgc ggcggctcct gcaggttccc cgaagacgcc
      121 ggtcttccgg gcaagctcgg gaatcgcgga caggatctcc tcgtcggaga ccgccaccat
      181 gtcgccctcg gactcgcgta ccgcccgaag cgccttggcc cagttccgcg gcgcgccgac
      241 agatatgctg tccgctactg tatccgcagg cccgaatgcc acgcgctccg ccttgctttt
      301 gaacgcatcg tagatcggct tcgcgccctg cgcctgaacg ccgacgatct tcggcatccg
      361 cgagatcagt cccatgcgaa gcagatcgct gaaccccttg tacaatccgc cgatgcagca
      421 gccgtcgccc acagagatga acaccctgtc cgggagatcc cagcccgtct gctcagctat
      481 ttcgagagcg caggtcttct tcccttccac caggtacgga ttgatcgcgc agttccggtt
      541 gtaccatccg tacttgttga tagccgccgt cgccaggttg aagacgtccg catagtcgcc
      601 cttcaccagg acaaccgtgg cgccgtagac cagaagctgc gtcaccttgg cgacaggcgc
      661 cttctcagga acgaagatga agctgcgaag cccccccacc gccgcgaacc ccgagagtga
      721 actcgcggcg tttccggtgg aggcgcaggc gatcgtcctc tggttgatgt cgatcgcgcg
      781 ggcaacgccc accgcgctgg cgcggtcttt gagcgaccct gtcggattcc gcccgtcgtc
      841 cttcaccatg aactccctca cgccgtactc ctcggcgacc gcaggacatt cgtacagcgg
      901 agtccaacca accgcaagat ggggaatgct ccccttgtcc acgatcggca gaatttcctc
      961 gtaacgccac agcgaccgct cgcgtcctgc aagcgccccc ttcgtcatcg tacgagccgc
     1021 cgattcataa tcgtacagaa cgtcgagcgt gccgtcgagt ccgcagaccg ggcaggtgta
     1081 ctccacctct cccggagcat acgaagctcc gcacagaaca catttcaact caactggctg
     1141 cgccatgtac tttcctcctt ttttcttcct tcacggaaac gcgtttcttt ccttgcccgc
     1201 agagagtccg ggacgccaag aggcgttccg ccatcgcaaa gtaccctctg cacgaatcga
     1261 caagcgcgtc tatttctata tactcatcta ctgtgtgcac caactcgcgc cgggagggtc
     1321 cgtacaccac ggtcggaagc aaccgctcgg ctgcgtaatg gcagccgttg gtcccgaagc
     1381 caggacgcgc cgacacatcc ggacagagcc ccgcttcctc cagcgcggcc gaaagcacgc
     1441 gcaaaaaagg cgcgtccgag gggatcgtcc acgcaggggc gtaatggctc cccctgatgg
     1501 gggcccccgt ataacagcga tcctccatca cagggtagga gaccttcgcc cggagctccg
     1561 gaagaacacg aaaagcctcg tccacgatct cctcgaactg cctgacgacg ccctccagcg
     1621 tctccccgag cagcagccgc ctgtcgaaaa cagctgtgca tcgctcggga atcgcgctgc
     1681 tgtcgggacg ggcctcccgt tgagaactct ctctgttgta tgacggtgag gaaaaaagat
     1741 tggtgaggac aagaattccc tcgccgagga atgcatcgtg cggaggaata aagcggttct
     1801 taagaaagga gagaaactcg accatgacgt ctgccgcgtt taccccgaac ttaggatgcg
     1861 aggagtgcgc cattcggcca aaaacatcga cgcgcatctc ggccctccct ctctggcctc
     1921 gttcgagcaa gaggccggag gcctcgccga caatcacgca gtccgggtcg gcaaaatctc
     1981 caatagcctt cgaagcgacc cgttcaaacg tctcctggtg caccgctccg gcgacaaaga
     2041 gcctgccccg cagagcgccg ctccggtctc gtttcagcag agacgcggca agcatcatcg
     2101 aggcaagcgc gcccttctgg tcggaggcgg ctcttccata aatgcgattc tcctcgatga
     2161 acgcgccgaa ggggtacttg gaccactcgg cgacgtctcc cgcatccaca tggtccatct
     2221 gcgcagtgag gagaagcctc ggcccctccg cggagaatat gatctctccc acgacgttgc
     2281 cgtatgaatc gatcacggcg gagtcaaaac cgagggaacg catgctctcg gcgacgaaac
     2341 gggcgacgtc gccctcacgg cccgacacgc cgggtctgcg aaggagctcc tggcaaagct
     2401 gaatcagatc gtcccgccct acggaagcaa cagcctgccg caggttccga acaccgaact
     2461 catccatgcc atcgccccct tgctctccgt gtacgctctc taccttctct tgctaccaag
     2521 atacaccaga tagtattatt tgcaagaagg aaaaaatgat ataatacttt aggaaaacct
     2581 atatcaagga ggcaacgcat gaatcttcac cagctgagga tattctgcac aatcgtggaa
     2641 gagggctcct tccgccaggc tgcggagaag ctcttcctgt cgcagccctc cgtaagccag
     2701 caggtcg
//
LOCUS       DAOTIL010000323         2699 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminobacteriaceae bacterium isolate AMR_MDS_3026
            NODE_44722_length_2699_cov_4.053328, whole genome shotgun sequence.
ACCESSION   DAOTIL010000323 DAOTIL010000000
VERSION     DAOTIL010000323.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868050
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminobacteriaceae bacterium (wastewater metagenome)
  ORGANISM  Aminobacteriaceae bacterium
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae.
REFERENCE   1  (bases 1 to 2699)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2699)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:19:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,224
            CDSs (total)                      :: 3,198
            Genes (coding)                    :: 3,184
            CDSs (with protein)               :: 3,184
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2699
                     /organism="Aminobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_44722_length_2699_cov_4.053328"
                     /isolate="AMR_MDS_3026"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:3034609"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1132
                     /locus_tag="P5201_07450"
     CDS             <1..1132
                     /locus_tag="P5201_07450"
                     /inference="COORDINATES: protein motif:HMM:NF018452.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF1214 domain-containing protein"
                     /protein_id="HRV98406.1"
                     /translation="NDTLSSTAWLDLRAEPMVLTVPPLPDRYYSFQFVDLYTSNFAYV
                     GARTIGSSGGVFLIAGPEWNGKTPRTVDALFQSEGNFVLCFGRTALNGPDDLPNVRAI
                     QQEYTLEPLSKFLGTPPPEATPSEVFPLYVKIAADSVHFISYLNFLMGQAKMHPSEEP
                     LFERFGLVGIGPSFYFNANDFDATIRDAIDDGIVSALEQIEESQSLLHEERNGWTIFR
                     QIFGDRERLQGLYLVRAGAAKAMLYGDDAEEVSYLTANLDEDGDVLDASTRSYALTFA
                     KEELPPVDAFWSITMYSLPDQALVQNPLDRYSLGSKSPSLVYGADGSLTLYLSKESPG
                     ESREGNWLPAPDGPFSLTLRMYLPKQDAIPPHYVPPPLRKVR"
     gene            1438..>2699
                     /locus_tag="P5201_07455"
     CDS             1438..>2699
                     /locus_tag="P5201_07455"
                     /inference="COORDINATES: protein motif:HMM:NF018561.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1254 domain-containing protein"
                     /protein_id="HRV98407.1"
                     /translation="MKKRKGLTWYLFLFAVLLLPLSGCSLTNSTENSNEARALAEEAY
                     IFAYPMLENYRTMQVQAISPDRFNRFKHATEIMGPEYKDIVRPNNDTLYSTLWLDLRA
                     EPMVLHIPPITDRYFSFQFVDMYTHNFAYTGVRTTGTRGGVFMVAGPEWNGEKPAGVN
                     EVFRSEGNFVLCLVRTALEGQEDLENVHAIQRRYGVEPLSAFLGVNPPAPSPMDVFPL
                     YLQPAAESVHFINYFNFLLGRLKVHPSEEEQIERFGAIGIGPGFYFNADNFSPEIRAA
                     IEAGIASAIKQIEASREILGERKNGWVLTRRIFGNRERMQGLYLVRAGAARMGLYGND
                     LEEAYYPTGNLDADGELFDASKHSYVLRFEKDEIPPVDAFWSITMYNLPEQLMVENPI
                     GRYSIGDRTEGLTFGEDGSLELYFSKTSP"
BASE COUNT          552 a          762 c          767 g          618 t
ORIGIN      
        1 taacgacacg ctttcctcga ccgcctggct cgatcttcgg gccgagccca tggtgctcac
       61 ggtgccgcct ctcccggacc gctattattc gttccagttc gtcgacctct ataccagcaa
      121 cttcgcctac gtcggggcca ggactatcgg ttcgtctgga ggggttttcc tgatcgccgg
      181 tcccgagtgg aacgggaaga cgcctcgtac tgtcgatgct ctcttccagt ccgaagggaa
      241 ctttgtactc tgtttcggcc gtacggctct caacggcccg gatgacctgc cgaacgtgcg
      301 tgcgattcag caggagtaca cgcttgagcc tctgagtaag tttctcggga ctccgccccc
      361 cgaggcgact ccttcggaag tctttccgct gtacgtgaag atcgccgcgg attctgttca
      421 cttcatctcc tatctgaact tcctgatggg gcaggcgaag atgcatccct ccgaagagcc
      481 gctgttcgag cgtttcggcc ttgtgggaat cggtccgtca ttctatttca atgcgaacga
      541 ttttgatgcc acgatacggg atgcgatcga cgacgggatc gtctctgcgc ttgaacagat
      601 cgaagagtcg cagtcgctgc ttcacgagga gagaaacggc tggacgatct tcaggcagat
      661 cttcggtgac agggaacgct tacaagggct ttatctcgtc agggccggag ccgccaaggc
      721 gatgctctac ggcgacgacg ctgaagaggt ctcttacctg accgcgaatc ttgatgagga
      781 cggcgacgtt ctggacgcct caacgcgttc atacgctctc accttcgcaa aggaggaact
      841 gccccccgtc gacgcattct ggtcgatcac aatgtactcg ctgcctgatc aggcgctggt
      901 acagaatccg ctggacaggt actctcttgg aagcaagagc ccttcgcttg tctatggcgc
      961 cgacggctcg ctgacgttat atctctccaa ggaatccccc ggcgagtcga gggaggggaa
     1021 ctggcttccc gctcccgacg gtcccttctc gctgacgctt cggatgtacc tgccgaaaca
     1081 ggacgcgatc ccgccgcact atgtgccgcc tcctttacga aaagtgcgct gaaaaagcga
     1141 tatccggaga gagcctccct gatcaggagg ctctctcttt ttcaatgccg gatctcattc
     1201 cgggcaaaaa tgcgagcgaa gggttccgag gaatcgcttc atcggttgct tggggatcgc
     1261 tttgcataaa caaccgacct ccggagtcga ctcttcagtt ggagggtttt ggtcgcatgt
     1321 ttttggggcc gaatttatgc ctgaaaatct gtttttctga tacaatggag ctacgtattt
     1381 ttactgacct cagcagacct ctatcctggg ggagagcaac aagaaaggaa gattactatg
     1441 aaaaaacgga aaggactgac gtggtatctg tttctttttg cggtgctcct tctgccgctc
     1501 tcaggatgtt ccctgacgaa cagcacggag aactccaacg aagcccgcgc gcttgccgag
     1561 gaagcgtata tcttcgcgta cccgatgctt gagaactaca ggacgatgca ggttcaggcg
     1621 atcagcccgg atcgtttcaa ccgtttcaag cacgcaacgg agatcatggg gcccgaatat
     1681 aaggatattg tccgtccgaa caacgatacg ctgtactcca cgctctggct ggatctgagg
     1741 gcagagccga tggtgctgca tattcctccg ataaccgacc gctatttttc attccagttt
     1801 gtggacatgt atacgcataa cttcgcctac accggcgtcc ggacgaccgg gacaagaggc
     1861 ggcgtgttca tggtcgcggg tccggaatgg aacggtgaaa aacctgccgg agtgaacgag
     1921 gtcttccggt cggagggcaa tttcgtcctc tgcctggtac ggactgcgct tgaaggacag
     1981 gaggacctgg agaatgtgca cgccattcag agacggtacg gtgtcgaacc tctgagcgct
     2041 ttcctgggag ttaacccgcc cgcgccttcc ccgatggacg tctttccgct ctatctgcag
     2101 cctgccgctg aatccgtcca tttcatcaac tacttcaact ttcttcttgg ccggctgaag
     2161 gtccatcctt cggaagagga acagatcgag cgtttcggcg ccattggcat cggccccggt
     2221 ttctacttta acgccgataa tttcagtccg gagattcgcg ccgcgatcga agcggggatt
     2281 gcgtccgcga tcaagcagat cgaggcctcc cgggagattc tcggagagag aaagaacgga
     2341 tgggtgctta ccagacgtat tttcgggaac agggagcgga tgcaggggct ctatctcgtc
     2401 cgcgccggag ctgcgcgtat ggggttgtac gggaacgacc tcgaagaggc atactatcct
     2461 accggcaatc tcgatgcgga cggcgagctg ttcgacgctt cgaagcactc gtatgttctc
     2521 cggttcgaga aggatgagat accgccagtc gatgcgttct ggtccatcac catgtacaac
     2581 cttccggagc agctgatggt tgaaaacccg atcggccggt actccatcgg cgaccgtacg
     2641 gaggggctga cgttcggcga ggatggctcc ctggagctct acttcagcaa gacttcgcc
//
LOCUS       DAOTIL010000324         2693 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminobacteriaceae bacterium isolate AMR_MDS_3026
            NODE_44854_length_2693_cov_3.376042, whole genome shotgun sequence.
ACCESSION   DAOTIL010000324 DAOTIL010000000
VERSION     DAOTIL010000324.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868050
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminobacteriaceae bacterium (wastewater metagenome)
  ORGANISM  Aminobacteriaceae bacterium
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae.
REFERENCE   1  (bases 1 to 2693)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2693)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:19:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,224
            CDSs (total)                      :: 3,198
            Genes (coding)                    :: 3,184
            CDSs (with protein)               :: 3,184
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2693
                     /organism="Aminobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_44854_length_2693_cov_3.376042"
                     /isolate="AMR_MDS_3026"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:3034609"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..467
                     /locus_tag="P5201_07460"
     CDS             <1..467
                     /locus_tag="P5201_07460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014807623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="LamB/YcsF family protein"
                     /protein_id="HRV98408.1"
                     /translation="VHLEHVKAHGALYNQAAKDLAMAKAIARATRDAGGNLILLGLAN
                     SLFEQAAADEGVLYAAEAFADRGYMDDGSLVPRSKPGAFIHDPDEAAMRMVRLVKEGV
                     VRSAEGNDIKLKAHSVCLHGDNPSAVELARHIRKTLQDNGVVISTIREVLEG"
     gene            464..1261
                     /locus_tag="P5201_07465"
     CDS             464..1261
                     /locus_tag="P5201_07465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014807622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative hydro-lyase"
                     /protein_id="HRV98409.1"
                     /translation="MKASDYANSSPREVRQLIREGKWTLPTPGMCKGHVQGNLVVLPR
                     DLAYDFLVFAQRNPKPCPILDVTEPGDPEPKIVAPGADISTDIPRYRVWKDGVCVDEP
                     TDVKKYWRDDLVGFLLGCSFSFEGALLEAGIPVRHIEQNRNVPMYVTNIQCTPAGALN
                     GPVVVSMRPIPAAMVPKAVLCTGRFPAVHGSPIHIGDPSQIGIADVNKPDLGDAVDIY
                     PGEVPVFWACGCTPQAAIMAVKPPFCITHSPGHMFVADPKDADYAVF"
     gene            complement(1355..>1495)
                     /locus_tag="P5201_07470"
     CDS             complement(1355..>1495)
                     /locus_tag="P5201_07470"
                     /inference="COORDINATES: protein motif:HMM:TIGR00786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter permease DctM/Q"
                     /protein_id="HRV98410.1"
                     /translation="GIAPKGITMGDIYRSVIPFVITQLVCLALCIIFPPLITWLPGIL
                     FN"
     assembly_gap    1496..1505
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<1506..2674)
                     /locus_tag="P5201_07475"
     CDS             complement(<1506..2674)
                     /locus_tag="P5201_07475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013047866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0031317 - tripartite ATP-independent
                     periplasmic transporter complex [Evidence IEA];
                     GO_function: GO:0015556 - C4-dicarboxylate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015740 - C4-dicarboxylate transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter large permease subunit"
                     /protein_id="HRV98411.1"
                     /translation="MDIGYISVLLFGSMFLFLLLGLPVAFVLGGLATVFTAIFWGPES
                     LFIIVARTYSMMSTTTLVACPLFVLMATVLERSGVSEDLYDMMYRWMGSLKGGLAIGT
                     VIACTLIAAMSGIASTGVVMMGMMALPAMLKRGYDKRLATGCILAGGVLGPLIPPSIA
                     LVLYGTIAQVSIGALFAGGMSAGLLCSVLIIGYILIKCHLNPEMGPAIPEEERADWGA
                     KMKSLKGIFLPMLLVFSVLGSIYSGIATPTEAAAVGALGAIVCSALHGRLTWKLLRDA
                     SYATIKVQGFMMWILFAAQAFAAVYMGMGASRMVVGLVKEYQVGWWTMLIGIQVVWFL
                     LGFVIDAWSILMITSPIILPLLPIYGFNPLWLGVLYAVNTQTGYLTPPFGTMLFM"
BASE COUNT          573 a          810 c          821 g          479 t
ORIGIN      
        1 gcgtgcacct cgaacatgtg aaggcgcacg gcgcgctcta caaccaggct gcgaaggacc
       61 tggcgatggc gaaggctatc gcgcgcgcca ctcgcgacgc gggcggcaat cttatccttc
      121 ttggtctggc caactcgctc ttcgagcagg ctgccgcaga tgaaggcgtg ctctacgcag
      181 ccgaggcgtt cgccgacaga gggtatatgg acgacggcag cctcgtgccg cgcagcaaac
      241 cgggcgcttt tatccacgat ccggacgaag ccgccatgcg gatggtccgg cttgtgaaag
      301 agggagtcgt ccgctccgcg gaggggaacg acatcaaact gaaggcgcat tcggtctgcc
      361 tgcacgggga taacccctcg gcggtggagc tcgcccgtca tatcaggaag acgttgcagg
      421 acaacggcgt cgtcatcagt actattcggg aggtgcttga aggatgaaag cgtccgatta
      481 tgcgaatagc tcgccccgcg aggtgcgcca actgatccgt gaagggaagt ggacgcttcc
      541 cacgccggga atgtgcaagg gacacgtgca gggaaacctt gtcgtcctgc cgcgcgacct
      601 cgcctatgac ttcctcgtct ttgcccagag gaatccgaaa ccctgcccga tcctcgacgt
      661 caccgagccg ggcgatcccg agccgaagat tgtggcgccg ggggcggaca tttcgacgga
      721 cattccgcgc taccgcgtct ggaaggacgg cgtttgcgtc gacgaaccta ccgatgtaaa
      781 gaagtactgg cgcgacgatc tcgtcggttt tctgctgggg tgttcgttct ccttcgaggg
      841 agcgctgctt gaggcgggca ttcctgtgcg gcacatcgag cagaaccgga acgtcccgat
      901 gtatgtcacc aacattcagt gcactcccgc gggcgcgctc aacggccccg tggtggtgag
      961 catgcggccg attcccgccg cgatggtccc caaggcggtc ctctgcaccg gccgtttccc
     1021 ggcggtgcac ggttccccca tccatatcgg cgatccgtcg cagatcggca ttgccgacgt
     1081 caacaagccc gacctgggcg acgctgtgga tatctatccg ggagaagtcc ccgttttctg
     1141 ggcgtgcgga tgcaccccgc aggcggccat catggcggta aagcccccgt tctgcatcac
     1201 gcactcgccg gggcacatgt tcgtcgccga cccgaaggac gccgactacg cggtttttta
     1261 gaaatcagag aagcagtttc cccgtacaca aaagaggagg ccggaaggcc tcctcttttt
     1321 attcgttctt ttcccctttt tattccccgc gctcctaatt gaagagtatg ccggggagcc
     1381 aggtgataag cggcggaaag atgatgcaca acgccagaca caccaactgc gtgatcacga
     1441 aggggatgac ggagcggtag atgtctccca tcgtgattcc cttgggtgcg atgccnnnnn
     1501 nnnnnatcat gaagagcatg gtgccgaagg gcggcgtcag gtatcccgtc tgcgtgttca
     1561 ccgcgtagag gacgcccagc cacagagggt tgaatccata gataggcagc agggggagaa
     1621 tgatgggcga ggtgatcatg aggatgctcc acgcgtcgat cacgaagccg agcaggaacc
     1681 agacgacctg gatgcctatc agcatcgtcc accagccgac ctgatactcc ttgacgagtc
     1741 cgacgaccat ccgcgaagcg cccataccca tgtagaccgc cgcgaaagcc tgtgcggcga
     1801 acaagatcca catcatgaat ccctgcacct tgatcgttgc gtacgacgcg tctctgagga
     1861 gcttccaggt cagtcttccg tggagcgcgc tgcacacgat ggcgcccaga gcgcctactg
     1921 cggcggcctc ggtcggcgtg gcgatgccgc tgtagatcga ccccaaaacc gagaagacca
     1981 gcagcatggg gaggaagata cccttgaggg acttcatctt cgccccccaa tcggcgcgtt
     2041 cctcttccgg aatggcaggc cccatttcag ggttgaggtg gcatttgatc aggatgtagc
     2101 caatgatcag aacggagcag agcaaccccg cgctcatgcc cccggcgaaa agagcgccga
     2161 tggagacctg agcgattgtt ccgtagagca ccagggcgat gctcgggggg atcagcggtc
     2221 caagaacgcc gcccgcgagg atgcagcctg tggccagcct tttgtcgtac ccgcgtttga
     2281 gcatcgcggg aagggccatc atgcccatca tgacaacgcc ggtggaggcg atgcccgaca
     2341 tggccgctat caaggtgcac gctatcaccg tgccgatagc aagccccccc ttgagagagc
     2401 ccatccagcg gtacatcata tcgtacagat cttcagaaac cccggagcgc tccagcacgg
     2461 tcgccatcag gacgaaaaga gggcaggcca ccagcgtcgt ggtcgacatc atggagtacg
     2521 ttcttgccac gatgataaag agggactccg ggccccagaa aattgcagtg aacaccgttg
     2581 ccagtcctcc gaggacgaac gcgacgggaa gcccgagcag caggaagaga aacatgctgc
     2641 cgaagagaag aacggagata tatcctatgt ccataaagag cacctcagtt ctc
//
LOCUS       DAOTIL010000325         2687 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminobacteriaceae bacterium isolate AMR_MDS_3026
            NODE_44981_length_2687_cov_3.305091, whole genome shotgun sequence.
ACCESSION   DAOTIL010000325 DAOTIL010000000
VERSION     DAOTIL010000325.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868050
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminobacteriaceae bacterium (wastewater metagenome)
  ORGANISM  Aminobacteriaceae bacterium
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae.
REFERENCE   1  (bases 1 to 2687)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2687)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:19:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,224
            CDSs (total)                      :: 3,198
            Genes (coding)                    :: 3,184
            CDSs (with protein)               :: 3,184
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2687
                     /organism="Aminobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_44981_length_2687_cov_3.305091"
                     /isolate="AMR_MDS_3026"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:3034609"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..195
                     /locus_tag="P5201_07480"
     CDS             <1..195
                     /locus_tag="P5201_07480"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRV98412.1"
                     /translation="PAMLGGGKVFFVGNALVDLFLQSRNWPFGAAVAVALVVVMLVTV
                     TLYMRLTRRWAGARDEVALM"
     gene            203..1156
                     /locus_tag="P5201_07485"
     CDS             203..1156
                     /locus_tag="P5201_07485"
                     /EC_number="4.2.3.5"
                     /inference="COORDINATES: protein motif:HMM:NF013432.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004107 - chorismate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate synthase"
                     /protein_id="HRV98413.1"
                     /translation="MRTYAIAVYLFLYIPIGVIAIFSFNAGRHAAQFQGFSTQWYGKA
                     LSNPFVMEALQTSATVATVSAALATVAGTLAARMLEELGVAVRGAVESIGGVAVRTPE
                     TFEEWTRASLSELGVTRPEDEETLKKRILQAAEEGDSLGGTFVLSITGLPPGVGSFAE
                     WDERLDGLLGQAILSIPGVKGVEFGEGFRSADLPGSRLHDPIVVDGGRWTRPSNNAGG
                     LEGGMSNGGEIRLRAVMKPIPTMKKGLPSFDTVSDRPCAGHSERSDVCAVPSACVVAE
                     AMAAWTVACAVTAQFGNDRMDDLAQRFASYKQRAERWNLHA"
     gene            1149..1712
                     /locus_tag="P5201_07490"
     CDS             1149..1712
                     /locus_tag="P5201_07490"
                     /inference="COORDINATES: protein motif:HMM:NF013375.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate kinase"
                     /protein_id="HRV98414.1"
                     /translation="MHSGNICIGGFMCSGKTSVGRELSRRLGWEFLDTDVVIEQRAGM
                     PVPRIFAELGEPVFRRFEFEVVSEAVGGSNRVVSLGGGALVNTELRNRVLANACLVIL
                     DVRAETVLCRAERQKGARPLLDSGTVHSLMAKRTSAYAACHLRVATDEFPVNAVADRI
                     LANIPLTGTLTGTDTIRAAAETLCVPA"
     gene            complement(1657..>2687)
                     /gene="pheA"
                     /locus_tag="P5201_07495"
     CDS             complement(1657..>2687)
                     /gene="pheA"
                     /locus_tag="P5201_07495"
                     /EC_number="4.2.1.51"
                     /inference="COORDINATES: protein motif:HMM:NF008865.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004664 - prephenate dehydratase activity
                     [Evidence IEA];
                     GO_process: GO:0009094 - L-phenylalanine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="prephenate dehydratase"
                     /protein_id="HRV98415.1"
                     /translation="ALLSQRADVAREVGRAKGDAPVYDPAREGEVIARAAEACPGMDR
                     SAVERIYREIISVCRGVQHRPKVAFLGPEGSFSHEGALAAFGGAIDLLPCLSFVDVFT
                     AVEKRSADWGVVPAENSLEGTVVPTMDAFASQAGLLDSSSHNNICIHSEVSIAIDHHL
                     ASPEKNLDAIREVFSHPQALGQCREWLRTNLPSASQIPAGSTSAAAASVAGAPGRAAV
                     CSALAARNNGLNILAEKIQDRGRNTTRFWTVGFGTPRKGERNKTSFLFSVEHRPGSLF
                     RAMEPLYLNGLNLTHIQSRPLSGSVFEYYFFIDVEGHIDEPHVRKALDVMRERTAFLR
                     LLGSYPCL"
BASE COUNT          446 a          893 c          892 g          456 t
ORIGIN      
        1 cccgccatgc tcggcggcgg caaggtcttc ttcgtcggca atgcgctggt cgacctcttc
       61 ctgcaatcgc gcaactggcc gttcggggcg gcggtcgcgg tcgcgctggt cgtcgtcatg
      121 ctcgtcaccg tcaccctcta catgcgcctt acgcggcgct gggcgggtgc gcgtgacgag
      181 gtcgcgttga tgtgagggcg cgatgcggac ctatgcgatc gctgtctacc tcttcctcta
      241 catcccgatc ggggtgatcg cgatcttttc cttcaacgcg ggccgccatg ccgcgcagtt
      301 ccagggcttt tcgacccagt ggtatggcaa ggcactgtcg aaccccttcg tgatggaggc
      361 gctgcagacc tccgccaccg tcgcgacggt atcggccgca ctggcgacgg tcgcgggaac
      421 gctggcggcg cggatgctcg aggagctggg cgtcgcggtc cgcggcgcgg tcgagtcgat
      481 aggaggcgtc gcagtccgca cccccgagac cttcgaggag tggacgcgcg cttcgctctc
      541 cgaactcggc gtcaccaggc cggaggatga agagacgctg aaaaagcgca tccttcaggc
      601 agccgaagag ggcgacagcc tcggggggac cttcgtcctc tcgatcaccg gcctcccccc
      661 cggcgtcggg agcttcgcgg agtgggacga gcggctcgac gggctgctcg gacaggcgat
      721 cctctccatc cccggcgtca agggcgtcga gttcggcgag ggcttccgca gcgccgacct
      781 tcccggcagt cgtcttcacg accccatcgt cgtcgacggc ggacgctgga cccgcccgag
      841 caacaacgcg ggaggactcg agggcggtat gagcaacggc ggcgaaatcc gcctccgcgc
      901 cgtgatgaag ccgatcccga cgatgaagaa ggggcttccc tcgttcgaca ctgtatcgga
      961 tcggccctgc gccggacact cggagcgaag cgacgtctgc gcggttccgt ctgcctgcgt
     1021 cgtcgcggag gcgatggcgg cgtggacggt ggcgtgcgcg gtgactgcac agttcggcaa
     1081 cgacaggatg gacgacctcg cgcagcgttt cgcatcctac aaacagcgag cggaaaggtg
     1141 gaatcttcat gcatagcggg aatatctgca tcggcggctt catgtgctcg ggcaagacga
     1201 gcgtcgggcg ggagctttct cgcagactcg gatgggagtt cctggacacg gacgtcgtca
     1261 tcgagcagcg ggcggggatg cccgtcccca gaatcttcgc cgagcttggc gagccggtct
     1321 tccggcggtt cgagttcgag gtggtctccg aagcggtcgg cgggagcaat cgcgtcgtgt
     1381 cgctgggcgg cggcgcgctg gtgaatacgg agctgcgaaa cagggtgctc gcgaacgcat
     1441 gcctcgtgat cctcgacgtc cgggccgaga cggtgctctg ccgggcggag cggcagaaag
     1501 gcgcgcgccc gctgctcgac agcggcacag tccattcgct gatggcgaag cggacctccg
     1561 cctacgctgc gtgccacctc cgggtggcca cggacgagtt tcccgtgaac gccgtggccg
     1621 accggattct ggcgaacatc cccctgacgg ggacgcttac aggcacggat acgatccgag
     1681 cagccgcaga aacgctgtgc gttcccgcat gacgtcgagc gccttccgca cgtggggctc
     1741 gtcgatgtgc ccctcgacgt cgatgaagaa gtagtactcg aagacgcttc ccgaaagggg
     1801 ccgcgactga atgtgggtaa ggttcagccc gttcagatag agcggctcca tggcccggaa
     1861 aagactcccc ggccggtgct ccacgctgaa cagaaacgac gtcttgttcc tctccccctt
     1921 ccggggggtt ccgaatccca cagtccagaa acgcgttgtg ttccgccccc tgtcctgaat
     1981 cttctccgcg aggatattca ggccgttgtt ccgtgccgcg agcgcgctgc acaccgcggc
     2041 gcgccccggc gccccggcga ccgacgccgc agccgcgctc gtgcttcccg cggggatctg
     2101 cgacgcagac ggcaggttcg tccgcagcca ctctctgcac tgcccgaggg cctgaggatg
     2161 cgaaaaaact tcacgaattg cgtcaagatt tttctccgga gaggcgagat gatggtctat
     2221 tgcaatcgaa acttcactgt gtatgcaaat attattatga gaagatgaat ccagcagccc
     2281 cgcctgagac gcgaacgcat ccatcgtcgg aaccaccgtc ccctccagcg agttctccgc
     2341 cggaaccacc ccccagtccg ccgagcgctt ctctacggcg gtgaagacgt cgacgaagga
     2401 gaggcaggga agcagatcga tcgccccgcc gaaggccgcg agcgctccct cgtgcgagaa
     2461 ggagccctcc ggcccgagga aggcgacctt cgggcggtgc tgcacgcctc tgcagacgga
     2521 gatgatctcg cggtagatcc tttcgaccgc gctccggtcc atcccggggc aggcttcggc
     2581 cgcgcgcgcg atcacctcgc cctccctcgc ggggtcgtac accggagcgt cccccttcgc
     2641 cctccccacc tcgcgggcga cgtccgcccg ctgcgaaaga agcgcgg
//
LOCUS       DAOTIL010000326         2681 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminobacteriaceae bacterium isolate AMR_MDS_3026
            NODE_45136_length_2681_cov_3.585301, whole genome shotgun sequence.
ACCESSION   DAOTIL010000326 DAOTIL010000000
VERSION     DAOTIL010000326.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868050
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminobacteriaceae bacterium (wastewater metagenome)
  ORGANISM  Aminobacteriaceae bacterium
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae.
REFERENCE   1  (bases 1 to 2681)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2681)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:19:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,224
            CDSs (total)                      :: 3,198
            Genes (coding)                    :: 3,184
            CDSs (with protein)               :: 3,184
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2681
                     /organism="Aminobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_45136_length_2681_cov_3.585301"
                     /isolate="AMR_MDS_3026"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:3034609"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..2469
                     /locus_tag="P5201_07500"
     CDS             <1..2469
                     /locus_tag="P5201_07500"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HRV98416.1"
                     /translation="ASDPTLVREGHAPSFASDLQLRLLSRRLGATLVFLLDTEGTDVS
                     TSEKLLEGMNFAFRSYFQDALQGRSGLMYAVGKATMSAGAYFSRPLVDRLGTVLGIVV
                     VKMDLEPVFGELFRTDRVVMHRQGEILSGPKGFESGRLEILQEDFLTLRCMSGEFQGG
                     GVRGPDGRMLPIVSLPLPGGGWELTKLLDPGSLLRYRRTLFAFYSLTSVLALLLLLRY
                     TQKNQLIGELKREVREREAAEQAERAARASAEQTNALLAEERNRAQMLAEQAEAASTA
                     KSSFLANMSHEIRTPLNAVLGMIGLLLDTKLDERQRHYAEVVQTSGDALLSLINDILD
                     FSKIEADRLEMEHIGFNLQTILEDTVEMLSQRAEQKGLHFGFFVEDDVPRFLKGDPGR
                     LRQILINLIGNAIKFTPSGEVILNVMLGSEVRETVSIRCTVRDTGIGIPEDRLPALFS
                     AFEQVDASTTRTFGGTGLGLAISKRLTELMGGHIGVESRIGEGSTFWFSIPFPVVPES
                     DVARTADPEFLQEACECEAPMGSRGKILLVEDTPVNQQVAVALLARMGYMADVAGNGE
                     EALTLLDSIPYDLVLMDIQMPVMDGFETTALIRSREASEKRERIPIVAMTAHALSGYR
                     EKCIEAGMDDYVSKPILPKELETALRRHLKKRSAEREEEIAAEEVAEEVTAEKGTENV
                     LETAESSQWEDIPLFDQADGAVRTAGDVVFLGKLLLLFADSNAGEEGRLREFAGRGEW
                     ESAKHAAHSLKGSSGNLGLLRLAEQARWTELALAEYMAGQSELDENALRSAVLSLADL
                     ASETMELARRQGTILGKEAEEAPS"
BASE COUNT          534 a          760 c          877 g          510 t
ORIGIN      
        1 gcctccgacc ccacgctggt cagggagggg cacgcgccgt cgttcgcctc ggatcttcag
       61 cttcgtttgc tgagccggag gttgggcgct accctcgtct ttctgcttga tacggaggga
      121 acggacgtct ccacatccga gaaactgctt gaaggaatga acttcgcctt ccgctcgtac
      181 ttccaggacg ctcttcaggg aagaagcggt ctgatgtacg cggtcggaaa ggccacgatg
      241 tccgcggggg cgtacttctc ccgtcccctt gtggacaggt tggggacggt cctcggtatc
      301 gtcgtggtca agatggacct ggaaccggtc ttcggcgagc ttttccgcac agacagggtt
      361 gtgatgcatc gtcaggggga gattctctcg gggccgaagg gtttcgagag cgggcgcctt
      421 gaaattttgc aggaagattt tttgacgctg cggtgcatgt ccggcgaatt tcagggggga
      481 ggggtgcgag gcccggatgg gcgaatgctg ccgattgttt cgctgccgct tcccgggggt
      541 ggctgggagc tgacaaaact gctcgacccg ggctctctcc tccgataccg aagaacgctt
      601 ttcgcctttt attccctcac ctccgtcctt gcgctgctgc tgctgctgcg ttatacgcag
      661 aagaaccagc tcatcggcga gctgaaacgg gaggtccgcg aacgggaggc ggcggaacag
      721 gcggagcgcg cggccagggc ctccgcggaa cagacgaacg cccttcttgc ggaggagcgc
      781 aaccgggcgc agatgcttgc ggaacaggct gaagccgcca gcactgcgaa gagcagcttc
      841 ctcgccaaca tgagccacga gatacgcact ccgctgaacg ccgtgcttgg catgatcggc
      901 cttctgctcg atacgaagct cgacgaacga cagcgccact acgccgaagt cgtacagacg
      961 agcggggacg cgctgctctc gctgatcaac gatatcctcg acttctcaaa gatcgaggcg
     1021 gaccgtctgg agatggagca tatcgggttc aatctgcaga cgatccttga ggatacggtg
     1081 gagatgctct cccaaagagc cgagcagaag gggctgcatt tcggcttctt cgtcgaggac
     1141 gacgtcccgc gtttcttgaa aggggatccc ggacgcctcc gccagatact gatcaacctg
     1201 ataggcaacg cgatcaagtt cactccctca ggcgaagtga tcctcaatgt gatgctcggg
     1261 tcggaggttc gtgaaacggt ctcgatacgc tgtaccgtcc gcgacacggg gatcggtatc
     1321 ccggaggatc gccttccggc gcttttcagc gccttcgagc aggtggacgc ctccacaacc
     1381 cgtactttcg gcggcacagg gctggggctg gccatcagca agcgcctgac ggagctgatg
     1441 ggcggacata taggtgtgga aagccgcatc ggcgagggct ccaccttctg gttttccatc
     1501 ccgtttccgg tcgtcccgga gagcgacgtt gcgcggacgg cggaccctga gttcctgcag
     1561 gaggcttgcg agtgtgaagc gccgatgggt tcaaggggga aaatacttct cgtggaggat
     1621 actccggtga atcagcaggt cgccgtcgcg ctcctcgcca ggatggggta catggccgac
     1681 gtcgcgggga acggcgagga agcgctgacg cttcttgact ccatccccta cgatctggtc
     1741 ctgatggata ttcagatgcc cgtcatggac ggatttgaga cgactgcgct catccgctcc
     1801 cgagaagcgt ccgaaaagag agagagaatt ccgatcgtcg cgatgacggc gcacgcgctc
     1861 tcaggatacc gcgagaagtg catcgaggcg ggaatggacg actacgtgag caaacccata
     1921 ttgccgaagg agctggagac agcgctccgg cggcatttga aaaaacgatc cgcggaacgc
     1981 gaagaagaaa ttgcagcaga ggaggttgcg gaagaggtga cggcggaaaa aggaactgaa
     2041 aacgtccttg agacggcgga gagttcgcag tgggaggata tcccgctctt cgatcaggca
     2101 gacggcgctg tgcggactgc cggggatgtc gtttttctgg gaaaactcct tcttctcttc
     2161 gccgactcga acgccgggga ggaagggcgt ctccgcgagt ttgccggacg cggggagtgg
     2221 gaatccgcga agcacgcggc gcactcgctc aaaggttcct ccggaaacct gggccttctc
     2281 cgtctcgccg aacaggcacg ctggacggaa ctggcacttg ccgaatatat ggcggggcaa
     2341 tccgaactgg atgagaacgc cctgcgttcc gccgttctct ccctcgcaga tctcgcctcc
     2401 gagacaatgg agctggcgcg gcggcaggga acaattctgg gcaaggaggc ggaagaagcc
     2461 ccctcctgat cctctacgcg gccgggctga attgtgctat ccttttcggc gaggattgac
     2521 cgaatgatgg agggatgaat catgacagca ccgcgccaag agagtcgagc cgcaggagag
     2581 ccgaacgcct gccgcgcgat cagcggccgg gaggcgcttc gcgcgcttgc ctgcatagcg
     2641 catcttatcc gggagatctt cccgggagac atgagtttcg c
//
LOCUS       DAOTIL010000327         2680 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminobacteriaceae bacterium isolate AMR_MDS_3026
            NODE_45165_length_2680_cov_2.734095, whole genome shotgun sequence.
ACCESSION   DAOTIL010000327 DAOTIL010000000
VERSION     DAOTIL010000327.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868050
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminobacteriaceae bacterium (wastewater metagenome)
  ORGANISM  Aminobacteriaceae bacterium
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae.
REFERENCE   1  (bases 1 to 2680)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2680)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:19:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,224
            CDSs (total)                      :: 3,198
            Genes (coding)                    :: 3,184
            CDSs (with protein)               :: 3,184
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2680
                     /organism="Aminobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_45165_length_2680_cov_2.734095"
                     /isolate="AMR_MDS_3026"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:3034609"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(93..1982)
                     /locus_tag="P5201_07505"
     CDS             complement(93..1982)
                     /locus_tag="P5201_07505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013048662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YgiQ family radical SAM protein"
                     /protein_id="HRV98417.1"
                     /translation="MKVPPVAGTRFFPVNRGEMEERGWAELDFLFVSGDAYVDHPAFG
                     TAVIARVLEAAGFRVGILAQPDWRSRDAFLSMGRPRYAALVSAGNLDSMLNKLTASKK
                     SRSTDSYSPGGQAGMRPDRATIAYCSRVKECWKDLPVIIGGVEASLRRFAHYDYWSDE
                     VRRSILFDSQADLLVYGMGELQIRQIADLLSRGVPVGRLQDLRGTVYRTNEFPEIRGK
                     FVEIPSYEDVLSDKKRFAEAFRMQDAEQDAFHGKTVVQRHGASFIVQNPPSPPLSTEE
                     MDAVYALPYTRTYHPMYEAAGGVPAIAEVRFSLTSHRGCFGSCSFCAIHYHQGRIIQS
                     RSHDSLLREARILTELPDFKGYIHDVGGPTANFRIPACDQQLVRGACRNRQCLSPTPC
                     PKLRADHSDYIDLLRKLRALPKVKKVFIRSGIRFDYLMADKKGNFLEELCAHHVSGQL
                     KVAPEHVSSRVLALMGKPGCEVYEKFRLAYAEMNEKLGKKQYLVPYFIASHPGATLED
                     AVELAEYLRDIHFQPEQVQDFIPTPGSLSTCMYYTGLNPFTGEAVPSAKERNERKMQR
                     VLLQYAEPKNREAVIQALEAAGRKDLIGTGPSCLVQPQHREPQKKSAPRPKGGTRSGK
                     PDGRR"
     gene            complement(1997..>2680)
                     /locus_tag="P5201_07510"
     CDS             complement(1997..>2680)
                     /locus_tag="P5201_07510"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRV98418.1"
                     /translation="AEVVALVNVELVADDGKTAKIVRGRGLSVISGPDSQGMLRVALP
                     NDRSKKQYSVAANTMSNPHSWPWYKIRHGGGKEGWIFGKFITVFNPRENTLSPTVLDR
                     ALHTFGSTKEALEGALGKPLKTASRKVTRSGVAADELTLTFNGVTAVLQERGGASEVK
                     SLTLSSSKHSLDGGFAVGSDRLAVLSILGLPNALDKGSEVYRLDANSGIRIHYESYKV
                     RTIHVGMLK"
BASE COUNT          474 a          853 c          840 g          513 t
ORIGIN      
        1 catcttcgtc gcggtgatgc ttacggaggc gggaccgtcg ctggtgcgtt cagtctcgct
       61 ccgcgtcgcg gcggcgcgcg agaaagcctg aactacctcc gtccgtccgg ttttccggag
      121 cgcgtccccc ccttggggcg gggcgcgctt tttttctgcg gctcgcggtg ctggggctgc
      181 accaggcagg acggcccggt accgatcagg tctttccttc cggcggcctc aagcgcctgt
      241 atgaccgcct cgcggttctt cggctccgcg tactgcagca ggacgcgctg catcttccgc
      301 tcgttcctct ccttcgcaga gggcacggcc tcccccgtga acgggttcag gccggtgtag
      361 tacatgcagg tcgacaggct ccccggcgtg gggataaaat cctgcacctg ctcgggctgg
      421 aagtgaatgt cgcgcagata ctccgccagc tccacagcgt cctcaagcgt cgctccgggg
      481 tgggaggcga tgaagtacgg cacgaggtac tgcttcttcc ccagcttctc gttcatctcg
      541 gcataggcca gacggaactt ctcgtacacc tcgcatccgg gcttccccat cagggccagc
      601 acgcgggacg aaacgtgctc gggcgccacc ttcaactgcc cgctcacgtg gtgcgcgcac
      661 agctcctcaa ggaaattgcc cttcttgtcc gccatgagat aatcgaagcg aatgccggag
      721 cggatgaaga ccttcttcac cttcggaagc gcgcgcagct tccggagcag gtcgatgtag
      781 tccgagtggt cggcgcggag tttcgggcag ggggtcggag aaaggcactg tctgtttctg
      841 cacgcccccc tgacgagctg ctggtcgcat gcggggatac gaaagtttgc agtcgggccg
      901 ccgacgtcgt ggatgtaccc cttgaagtcg ggcagctcgg tcagaatccg cgcctcccgg
      961 agcagggagt cgtggctgcg gctctggatg atccgcccct ggtggtagtg gatggcgcag
     1021 aaggaacagc ttccgaagca gcctcgatgg ctggtcaggc tgaagcgcac ctcggcgatc
     1081 gcgggaacgc cccccgccgc ttcgtacatc gggtggtagg tccgtgtgta cggaagcgcg
     1141 tagacagcgt ccatctcctc cgtcgaaagc ggcggcgagg gagggttctg tactataaac
     1201 gacgcgccgt gacgctgcac cacagtcttg ccgtggaacg cgtcctgctc ggcgtcctgc
     1261 atgcggaagg cctcggcgaa acgtttcttg tcggagagaa catcttcgta ggaggggatc
     1321 tccacgaact tgccgcggat ttcagggaac tcgttcgtcc tgtagacggt tccgcgaaga
     1381 tcctggagcc gtccgacagg gacgcccctg gagagcaggt cggcgatctg tcgtatctga
     1441 agctccccca tcccgtagac cagcagatcc gcctggctgt cgaagagaat ggaacgccgc
     1501 acctcgtcgg accagtagtc gtaatgggca aaccgccgaa ggctcgcctc tacgccgccg
     1561 ataatcacag gaaggtcttt ccagcactcc ttcaccctgc tgcagtaggc gatcgtcgcc
     1621 ctgtccgggc gcatccccgc ctgaccgccg ggagagtagc tgtcggtact ccggctcttc
     1681 ttcgaggccg tcagtttgtt gagcatcgag tcaagattgc ctgcggagac aagcgccgca
     1741 tagcgcggtc tccccatgga gaggaatgcg tcccgggagc gccagtcggg ctgcgccaga
     1801 atgcccacgc gaaaacccgc ggcctccagc acgcgggcga tcaccgcagt accgaaggcc
     1861 ggatggtcga cataggcgtc gccgctgacg aagaggaaat ccagctcggc ccagccgcgt
     1921 tcctccatct cccccctgtt cacggggaag aagcgcgtcc cggcaacagg gggaaccttc
     1981 atcgggggaa aagcgttcac ttcagcatac caacatgaat cgtcctgacc ttgtagcttt
     2041 cgtagtggat acggatgccg ctgttcgcgt cgagacgata gacttcgctc cccttgtcga
     2101 gggcgttcgg aagccccaga atcgagagga cggccaagcg gtcgctccca acggcgaatc
     2161 cgccgtcgag agagtgtttc gaggaggaga gcgtgagcga tttgacttcc gacgcacccc
     2221 cccgctcctg caacaccgcg gtcacgccgt tgaaggtcag cgtgagttcg tccgccgcga
     2281 cgcccgagcg agtgaccttc ctcgaggcgg ttttcagcgg tttaccgagc gcgccttcaa
     2341 gcgcttcctt tgtggaaccg aaagtatgca gcgcacggtc cagaactgtc ggcgacaggg
     2401 tattctcgcg ggggttgaag acagtgatga acttgccgaa aatccacccc tccttgcctc
     2461 cgccgtggcg aatcttgtac cagggccatg aatgcgggtt gctcatcgta ttcgcggcca
     2521 ccgagtactg tttcttcgaa cggtcgttgg gcagcgccac acggagcatt ccctgcgaat
     2581 ccggcccgga aatcacggaa agcccgcgcc cccggacgat ttttgctgtc ttgccgtcgt
     2641 ccgcgacaag ctcgacgttg accagggcga cgacttccgc
//
LOCUS       DAOTIL010000328         2676 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminobacteriaceae bacterium isolate AMR_MDS_3026
            NODE_45263_length_2676_cov_3.316291, whole genome shotgun sequence.
ACCESSION   DAOTIL010000328 DAOTIL010000000
VERSION     DAOTIL010000328.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868050
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminobacteriaceae bacterium (wastewater metagenome)
  ORGANISM  Aminobacteriaceae bacterium
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae.
REFERENCE   1  (bases 1 to 2676)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2676)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:19:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,224
            CDSs (total)                      :: 3,198
            Genes (coding)                    :: 3,184
            CDSs (with protein)               :: 3,184
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2676
                     /organism="Aminobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_45263_length_2676_cov_3.316291"
                     /isolate="AMR_MDS_3026"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:3034609"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..252)
                     /locus_tag="P5201_07515"
     CDS             complement(<1..252)
                     /locus_tag="P5201_07515"
                     /inference="COORDINATES: protein motif:HMM:NF018513.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter large permease subunit"
                     /protein_id="HRV98419.1"
                     /translation="MSDPTIATWILLGSFALFLVLKVPITFSLAVSSVLTAMYLKIPL
                     MAIVQQMVQGVNSFSLLAIPFFIIAGEMMGEGGISRRLIA"
     gene            complement(245..766)
                     /locus_tag="P5201_07520"
     CDS             complement(245..766)
                     /locus_tag="P5201_07520"
                     /inference="COORDINATES: protein motif:HMM:NF016200.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter small permease"
                     /protein_id="HRV98420.1"
                     /translation="MNAVTSFFRLLHTLLVILAKTLLAAMVLLIAANVFMRYVLNSGI
                     TWSEEVALVFVVWFTFIALALGVKQRLHISLCLLPEKISPKIDFLLSKLTDIVILFMG
                     YIMIHYGWILVQFTSRSILPATGVPASVLYFPLVLSGVLVLYEGGMNLLGLDKGDANM
                     DDKFSGRRCASDV"
     gene            complement(828..1805)
                     /locus_tag="P5201_07525"
     CDS             complement(828..1805)
                     /locus_tag="P5201_07525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008708881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter substrate-binding protein"
                     /protein_id="HRV98421.1"
                     /translation="MKKWILVLTALALVFAALPAAADTVVLRLGETHVADYPTTKGNY
                     EFARLVEERTGGRIKIEVYHSSQLGQEKAVIEGVQFGAIDFTRVSISPLSAFAPAFDA
                     LQMPYLYRGEEHMWKVLNGSIGEEFLASLEPANFVGLAWYDSGARNFYNSKKEIKSVA
                     DLKGMKIRVQESQLMMGLVSALGAVPTPMPFGEVYSALQTGVIDGAENNWPSYFSTSH
                     YEVAKYFTLDGHTRVPEILIASKISMDKLSKEDQEIIKQAAKDSMPYQIKLWKEFEKV
                     SEEKVRAAGSIITELTPEAVVEFQNAMQPMYDALSPELQEVVKKIRDVQ"
     gene            complement(1922..>2676)
                     /locus_tag="P5201_07530"
     CDS             complement(1922..>2676)
                     /locus_tag="P5201_07530"
                     /EC_number="5.3.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008708883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008880 - glucuronate isomerase activity
                     [Evidence IEA];
                     GO_process: GO:0006064 - glucuronate catabolic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="glucuronate isomerase"
                     /protein_id="HRV98422.1"
                     /translation="IWRRHQYFHGKGCRLSDYGIETPYSVEWTTEDVEGSFAALRRGK
                     TVTGDALARFRSAVLFEMLTMDANQGWVQQLHLGAMRNNNSRAFSRLGPNTGYDSMGD
                     FEQGRALVRLLDRLERTGKLTKTIVYSLNPKDNDMIPTILGSFQDGKIPGKMQMGSAW
                     WFADTKDGMTRQLRALSSVGLLSRFVGMLTDSRSFLSYPRHEYFRRILCDLLGSDVER
                     GELPEDYALLGSVVQDVCFNNAKEYFQLNFNR"
BASE COUNT          567 a          727 c          817 g          565 t
ORIGIN      
        1 tgcgatgagc cgccgtgaaa tacctccctc gcccatcatc tcgccggcga tgatgaaaaa
       61 aggaatcgcg agcagcgaga aagaattcac tccctgcacc atctgctgga ctatggccat
      121 gagtggaatc ttcaggtaca ttgcggtgag cacagaggaa acagccaggg agaaggtgat
      181 cggcaccttg aggacaagga aaagggcaaa gctgccgagg aggatccacg tagcgatggt
      241 cggatcagac atcggaggcg cacctcctgc cggagaattt atcgtccatg tttgcgtcgc
      301 ccttgtcgag tccgagcaga ttcattccgc cttcgtagag gacgagcacg cccgagagta
      361 cgagcggaaa atagagtacg gaggcgggaa ctcctgtcgc cggaaggata gagcggctgg
      421 tgaactggac gaggatccat ccgtagtgga tcatgatata ccccatgaag agaatgacga
      481 tatcggtcag tttggagagg agaaagtcga tcttcgggga gatcttctcc ggaagaaggc
      541 agaggctgat atgaagccgt tgtttgacgc cgagcgcaag agcgatgaag gtgaaccaga
      601 cgacgaagac gagcgcgacc tcctcggacc aggtgatgcc ggagttgagg acatagcgca
      661 tgaagacgtt ggcggcgatg agaagcacca ttgctgcgag cagcgtcttc gcgagaatga
      721 cgaggagcgt gtgcagcagc cggaagaacg atgtgacagc gttcacgggc agacctcctt
      781 cctgaaaaaa gggaagactc cctcaggagc cttccctttt cgagccgtta ctgtacgtcg
      841 cgaattttct tgactacttc ctgcagttcg gggctgaggg cgtcgtacat cggctgcatg
      901 gcgttctgga actcgacaac tgcctcgggt gtcagctcgg tgatgatcga acccgcggcg
      961 cggacttttt cttcggagac cttctcgaac tctttccaaa gtttgatctg atagggcatg
     1021 gagtccttcg cggcctgttt gatgatctcc tggtcttctt tggagagctt gtccatgctg
     1081 atcttgctcg cgatcaggat ctcgggaacg cgggtgtgcc cgtcgagagt gaagtacttc
     1141 gcaacctcgt agtggctggt ggagaagtag ctgggccagt tgttctccgc gccgtcgatg
     1201 acgccggtct gaagcgcgct gtacacttcg ccgaagggca tgggggtggg gacggcgccg
     1261 agagccgata cgaggcccat catgagctgg ctctcctgga cgcggatctt catccctttc
     1321 agatcggcga cggatttgat ctccttcttg gaattataga agtttcgggc gccggagtcg
     1381 taccacgcga ggccgacgaa gttcgccggc tcgaggctgg ccagaaactc ctctccgatc
     1441 gaaccgttga gtaccttcca catatgctct tcgccgcggt acagatacgg catctggagg
     1501 gcgtcgaaag cgggcgcaaa ggcggaaagc ggggagatgc tgacgcgggt gaaatcgatc
     1561 gctccgaact gaactccttc gatgaccgcc ttctcctgac cgagctgact gctgtggtag
     1621 acttcgatct tgatccgtcc gccggtgcgt tcctcgacga gcctggcaaa ctcgtaattc
     1681 cctttcgtgg tcggatagtc ggccacgtgc gtctctccca agcggagcac gacggtgtcg
     1741 gccgccgccg ggagtgccgc gaagacaaga gcaagcgctg tcagaaccag aatccacttc
     1801 ttcatttttt ctcctccttc atagtgtggt gatgaataac ctgcgattcc tatttattgt
     1861 aaatgcctcg cagggcaaaa gcaagctttt ttacacaata cgcgcactct tcgaccttgt
     1921 ttcacctatt gaaattcagc tgaaagtatt cctttgcatt gttgaagcag acatcttgaa
     1981 ctacagagcc gagaagcgcg tagtcctccg gcagttcccc tcgttccacg tcggatccga
     2041 gcagatcgca gaggatgcgg cggaagtact cgtgcctcgg ataggagagg aagctcctcg
     2101 aatcggtcag cataccgacg aagcgggaga gcaatccgac agaggaaagg gcgcgaagct
     2161 gcctcgtcat gccgtccttc gtgtcggcga accaccaggc ggagcccatc tgcatcttgc
     2221 cggggatctt tccgtcctga aagctgccga ggatggtcgg gatcatgtcg ttgtccttgg
     2281 ggttgagcga gtacacgatg gtcttcgtca gcttgcccgt ccgttcgagg cggtcgagaa
     2341 ggcgtacaag cgcgcgcccc tgttcgaagt cgcccatgga gtcgtacccc gtgttcgggc
     2401 cgagacggct gaacgcgcgg gaattgttgt tgcgcatggc tcccaggtgg agctgctgca
     2461 cccacccctg gttcgcgtcc atcgtgagca tctcgaagag cacggccgaa cggaagcgcg
     2521 cgagcgcgtc ccccgtgaca gttttccccc ttcgcagcgc cgcaaaggag ccctctacat
     2581 cctcggtggt ccattcgacg gagtagggag tctcgattcc gtagtccgag agtcggcatc
     2641 ccttgccgtg aaagtactgg tgcctgcgcc agatgc
//
LOCUS       DAOTIL010000329         2672 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminobacteriaceae bacterium isolate AMR_MDS_3026
            NODE_45359_length_2672_cov_3.773405, whole genome shotgun sequence.
ACCESSION   DAOTIL010000329 DAOTIL010000000
VERSION     DAOTIL010000329.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868050
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminobacteriaceae bacterium (wastewater metagenome)
  ORGANISM  Aminobacteriaceae bacterium
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae.
REFERENCE   1  (bases 1 to 2672)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2672)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:19:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,224
            CDSs (total)                      :: 3,198
            Genes (coding)                    :: 3,184
            CDSs (with protein)               :: 3,184
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2672
                     /organism="Aminobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_45359_length_2672_cov_3.773405"
                     /isolate="AMR_MDS_3026"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:3034609"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..575
                     /locus_tag="P5201_07535"
     CDS             <1..575
                     /locus_tag="P5201_07535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014806006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="diaminopimelate decarboxylase"
                     /protein_id="HRV98423.1"
                     /translation="GYGVEFAPEGTTPRIDAFTDAMMRALEEECRSAGVDRPRVIIEP
                     GRWIVSEAGITLYTVQAIKKIDGIVTYASIDGGMTDNPRPSLYGAKYWAVAAGKMNRE
                     ASAKVTVAGRCCESGDILIENLKTPPLERGDLLAVLNTGAYTFSMASNYNRLPRPAVV
                     LASEGRADVIVPRQSYEDLIRGESLPEHLR"
     gene            721..1878
                     /locus_tag="P5201_07540"
     CDS             721..1878
                     /locus_tag="P5201_07540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006300356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HRV98424.1"
                     /translation="MKKSLALFGVLLLLVFAGSAFAADVIKIGHTVSLTGGASMWGQS
                     EARALDMLAKKINEAGGVLGKQIEFVRYDNRNDGVEAVNVARRLVSDGVVAVIGPAQS
                     GNSIASAPVFERGKIPMVVTTATNPYVTIDQRSNKVRPFAFRPCFIDPFQGTVAARFA
                     VTDLGAKKGAILYDVGSDYGQWLARYFEEAFVAKGGEIVAKEAFRTEELDYRAQLGKM
                     KDLAPDVIFIPTSQKEAAMAAKQARDLGISAVLLGTDNWGSPDLIELGGSAIDGGYFV
                     NLTDLADPDIQEFVAEYKAAFGEEPVLPNPVMAQDALLMIVDAIARAGSVEGDAVAEA
                     LATIKDLKVTSGVLTINAEDHNPLDKPAVIQQVDVTNKTFKFVKKYVSVDD"
     gene            2039..>2672
                     /locus_tag="P5201_07545"
     CDS             2039..>2672
                     /locus_tag="P5201_07545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006300357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0015658 - branched-chain amino acid
                     transmembrane transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HRV98425.1"
                     /translation="MFLQTLITGLSIGGIYALMAVGYSLVFSVLNFSNFAHGAVIMLG
                     AYVGLALATKFSLGLGLVIAGSVAGAGIIAVLNERLAYSSLRRRKAPSLYLMISAMGC
                     AVFLENMVYATIGSRFYAFPEFFGRQIIDLWGSSVSLLDMGAFLFALAAILGLHLFIS
                     RTRTGIAIRAGVSDMTMCSLMGVDLDRLIAIVFLLAGAFAGVAGVFLGIKY"
BASE COUNT          521 a          786 c          846 g          519 t
ORIGIN      
        1 gagggtacgg ggtggagttc gcacccgaag gcacgactcc ccgtatcgac gcgttcaccg
       61 acgcgatgat gcgcgctctc gaagaggagt gtcgctcagc gggcgtcgac cgtccgcgcg
      121 tgatcataga gcccgggcgc tggattgtct ccgaggcggg gattacgctc tatacggtcc
      181 aggcgatcaa aaagatcgac ggcatcgtaa cctatgcgag tatcgacgga ggaatgaccg
      241 acaaccccag accctcgctc tacggggcga agtactgggc ggtcgccgcg ggaaagatga
      301 atcgcgaggc gtcggcgaaa gtgactgtgg cgggacgatg ctgcgagtca ggcgacatcc
      361 ttatcgaaaa tttgaaaacg ccgccgttgg agcgggggga tctgcttgcc gtgctgaata
      421 ccggcgcgta caccttctcc atggcgagca actacaaccg actcccgcgc cctgccgtgg
      481 tgctggcgag cgaggggaga gcggacgtga tcgttccccg gcagagctac gaggacctta
      541 tccgcggaga gtctctgccc gaacatttac gatagaactg gttcctgaat ttgtacgcgg
      601 aggtggggcc tgagaagaat cgttgcggga aaaggcggga atccgtgaaa cgggcttcgc
      661 gcgggtgatc aacccggcgc ggcggaacac gaaacacatc acaaggaggt aggaagagat
      721 atgaaaaaga gtcttgcgtt gtttggagta ctgcttctgc tcgtctttgc cggatcggcg
      781 tttgcggctg atgtcatcaa aataggccac acggtctccc tcaccggcgg cgcttccatg
      841 tgggggcagt ccgaggcccg ggcgctcgac atgctggcga agaagatcaa cgaggccgga
      901 ggcgttctcg gcaagcagat cgagttcgtc aggtacgaca accggaacga cggcgtggaa
      961 gcggtcaacg tggcccgtcg ccttgtttcg gacggagttg tcgccgtcat cggcccggcg
     1021 cagagcggca attccatcgc gtcggccccg gtcttcgaga ggggaaaaat tcccatggtc
     1081 gtgacgactg cgaccaaccc ctacgtcacg attgatcagc gctcgaacaa ggtccgtccc
     1141 ttcgccttcc gtccctgctt catcgacccc ttccagggaa ccgtggcggc acgtttcgcc
     1201 gtgaccgacc tcggcgcgaa aaagggcgcc atcctctatg acgtgggttc ggactacggc
     1261 cagtggcttg cacgttattt cgaagaggcc tttgtggcca agggcggcga gatcgtcgca
     1321 aaggaggcct tccgcaccga ggagcttgac taccgcgcgc agctcggcaa gatgaaggac
     1381 ctcgctcccg acgtcatctt catcccgaca agccagaaag aagccgccat ggcggcgaag
     1441 caggctcgcg atcttgggat cagcgccgtt cttctgggta ccgacaactg gggcagcccc
     1501 gatctcatcg agctgggcgg cagcgccatc gacggcggct acttcgtcaa cctgacggat
     1561 ctcgccgacc cggatatcca ggaattcgtc gccgagtaca aagctgcatt cggcgaggag
     1621 cccgtgctgc cgaacccggt tatggcgcag gatgcgctgc tgatgatcgt cgacgccatc
     1681 gccagagcgg gaagcgtcga gggcgacgcg gttgcggaag ccttggcaac catcaaggat
     1741 ctgaaagtca ccagcggcgt gctcaccatc aacgcggagg accacaaccc gctcgacaag
     1801 ccggcggtca tccagcaggt ggacgtaacc aacaagacct tcaagttcgt caagaagtac
     1861 gtttccgtcg acgattagca gcggcctgtt ttcacaggag cgtgctgaac acgttctccg
     1921 aacatcgtat cgaaaccgaa gggtgcggga atctctcccg cgcccttcat ccggaggccc
     1981 ggggactccg cggattcagc gttcctcccc aatatgctca aaggatgtgt gagccggttt
     2041 gtttcttcaa actctgatta ctggcctctc gattggaggc atctatgccc tgatggccgt
     2101 ggggtactcc ctcgtcttca gcgtgttgaa tttcagcaac ttcgcccacg gagcggtgat
     2161 catgctcggc gcctacgtgg gactggccct ggcgacgaag ttcagtctgg ggctgggact
     2221 cgtgatcgcg gggagcgtcg ccggagcggg gattatcgcg gtgctcaacg agcgcctcgc
     2281 ctattccagc ctccggcgga gaaaggcgcc ttcgctctac ctgatgatca gcgcaatggg
     2341 gtgcgcagtc tttttggaga acatggtgta cgcgaccatc ggctcccgtt tctacgcctt
     2401 tcccgagttc ttcggacggc agatcatcga cctgtggggg agcagtgtca gtctcctcga
     2461 catgggggcc tttctcttcg cgcttgccgc cattcttggg ctgcatctct tcatcagccg
     2521 gacgcggacg ggcatcgcca tccgcgcggg cgtctcggac atgacaatgt gctccctgat
     2581 gggggtcgat ctcgacagac tcatcgccat cgtcttcctt ctcgcggggg cgttcgccgg
     2641 agtggcggga gtttttctcg ggataaagta cc
//
LOCUS       DAOTIL010000330         2669 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminobacteriaceae bacterium isolate AMR_MDS_3026
            NODE_45441_length_2669_cov_2.938409, whole genome shotgun sequence.
ACCESSION   DAOTIL010000330 DAOTIL010000000
VERSION     DAOTIL010000330.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868050
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminobacteriaceae bacterium (wastewater metagenome)
  ORGANISM  Aminobacteriaceae bacterium
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae.
REFERENCE   1  (bases 1 to 2669)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2669)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:19:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,224
            CDSs (total)                      :: 3,198
            Genes (coding)                    :: 3,184
            CDSs (with protein)               :: 3,184
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2669
                     /organism="Aminobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_45441_length_2669_cov_2.938409"
                     /isolate="AMR_MDS_3026"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:3034609"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..381)
                     /locus_tag="P5201_07550"
     CDS             complement(<1..381)
                     /locus_tag="P5201_07550"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRV98426.1"
                     /translation="MIFPNYWMLFAILFPMTAAFILQLLHHFAPSKTGGWILLSCGIT
                     SLLTFGAAVSAISGVRVLRLGGWGADLGVLLVSDPLSAVFLFISAFGGAAAVWTTFTN
                     ISKSGPSAEQWRLCGLFFILWASLN"
     gene            complement(455..790)
                     /locus_tag="P5201_07555"
     CDS             complement(455..790)
                     /locus_tag="P5201_07555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014162633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation:proton antiporter subunit C"
                     /protein_id="HRV98427.1"
                     /translation="MAVMSFLTVSLLFALGLFTVLSQRNVFKTVIGLTLMESAVLLLL
                     ISWAYIPGGSAPILPLQGKGVDPLPHSFTLTAIVIGACDIALAFAFIMKLHRFERSVN
                     VDDFRRLKG"
     gene            complement(778..1773)
                     /locus_tag="P5201_07560"
     CDS             complement(778..1773)
                     /locus_tag="P5201_07560"
                     /inference="COORDINATES: protein motif:HMM:NF015970.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MnhB domain-containing protein"
                     /protein_id="HRV98428.1"
                     /translation="MNEHLLAFLYFFILLTAVVAVFLDDLLSSFISLSVTGVLMATVF
                     ALLRAPDVALTQAIINSGLVTSLFLVAYSRTQRTPPPDGECRECPFWLRWPGAALVSA
                     LFAAVLFRGLFVVSGERILGRASAHYLSHTLEETGALNVVAAILLDYRAFDTLGETTV
                     IFTAVFGISLLLSGGRYVHSGHGLSFIVKRGMALLTPFILLYAASILFLGHLTPGGGF
                     QGGSVFATVAILICVVYGRNFEAARISPKTKETFEAGGAVLFVFIGLLGIAWGGGFLA
                     NLSGPFSAGTPGSLFSGGNMLLLNLAVGMKVGAGLSSIFYTMIKILELEDDSWPS"
     gene            complement(1770..2096)
                     /gene="mnhG"
                     /locus_tag="P5201_07565"
     CDS             complement(1770..2096)
                     /gene="mnhG"
                     /locus_tag="P5201_07565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005659185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051139 - metal cation:proton antiporter
                     activity [Evidence IEA];
                     GO_process: GO:0098662 - inorganic cation transmembrane
                     transport [Evidence IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="monovalent cation/H(+) antiporter subunit G"
                     /protein_id="HRV98429.1"
                     /translation="MTPLLRFLGGISIFAGVFFSFAAVVAILRFPDVYTRLHGGTKAL
                     SGGAILILLGAMPYAGGVAGALKLFLIAVFFLVSNPMASHAIARACHRRTLANRPIDE
                     KKRDDV"
     gene            complement(2093..2350)
                     /locus_tag="P5201_07570"
     CDS             complement(2093..2350)
                     /locus_tag="P5201_07570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014162629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015075 - monoatomic ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0034220 - monoatomic ion transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="monovalent cation/H+ antiporter complex subunit
                     F"
                     /protein_id="HRV98430.1"
                     /translation="MILAALWGMVISGLIGFVRVVTGPSPADRLVAADALSVIFTMVL
                     VLLSLYIETPFLLDVALAFSMLSFADMLLMAKYFEHGELHK"
     gene            complement(2347..>2669)
                     /locus_tag="P5201_07575"
     CDS             complement(2347..>2669)
                     /locus_tag="P5201_07575"
                     /inference="COORDINATES: protein motif:HMM:NF014012.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="Na+/H+ antiporter subunit E"
                     /protein_id="HRV98431.1"
                     /translation="RTVAFVSFVPFFLFAMIKASWDVALQAFRPELSISPGTFVYTPS
                     PAFKGSIVALANAVTLTPGTLTVDILHGRRSLLVHALMAEGGEETLRNDVRRLEEGIA
                     GFTE"
BASE COUNT          733 a          751 c          761 g          424 t
ORIGIN      
        1 gttgagcgaa gcccagagga tgaaaaaaag accgcagaga cgccactgct ccgctgacgg
       61 ccctgatttg gagatgttcg taaaagtcgt ccacacggca gccgctcctc cgaaagcgga
      121 gatgaaaaga aaaaccgccg acagaggatc cgaaacaagg agaaccccca aatcggcgcc
      181 ccatcccccg agccggagca ccctgacgcc ggaaatcgcg gaaacggccg caccgaacgt
      241 cagcaacgag gtgataccac aggaaagaag gatccatcct ccggtcttcg agggtgcgaa
      301 atgatgcagc agttgaagaa taaaggccgc cgtcatcggg aagaggatag cgaacagcat
      361 ccaatagttt ggaaagatca tggccgcaac aatctccggt ctgcatcgca gacagggatg
      421 tcttcaggag agaagaagcg caagggttct ctcatcatcc tttgagcctc ctgaaatcat
      481 ccacgttcac actgcgctcg aaacggtgca gtttcatgat aaaggcaaag gccaacgcga
      541 tatcgcacgc gccgatgacg atcgccgtca gggtaaacga atgcggaagc gggtccaccc
      601 cttttccctg caacggcaga ataggggccg agccgcccgg gatatacgcc catgaaatca
      661 agagcagaag caccgccgat tccataagcg tcaaaccgat cacggtcttg aaaacgtttc
      721 tctgggaaag gacggtgaag agccccagcg caaagagcag cgagacggta agaaagctca
      781 tgacggccat gagtcgtcct ccagttcgag tatttttatc atggtgtaga agatcgagga
      841 aaggcccgcc cccaccttca tacctacggc caggttgagc agcagcatgt ttccgccgct
      901 gaacagagaa ccgggcgtgc ctgcggaaaa cggaccggaa agattggcca ggaagcctcc
      961 tccccacgca attcccagaa gtccgatgaa gacgaagagc accgcgcctc ccgcttcgaa
     1021 cgtctctttg gtctttgggg agatgcgcgc agcctcgaag ttcctgccgt agacgacgca
     1081 gatgagaatc gccactgttg cgaagacgct ccccccctgg aatccacccc cgggcgtcag
     1141 atggccgagg aagagaatgc ttgcggcata cagaagaata aagggagtca gaagcgccat
     1201 tccacgcttg acgatgaaag agagtccatg cccggagtga acatatcttc cgccggacaa
     1261 aagcagcgag ataccgaaga ccgccgtaaa aatgactgtg gtctctccca gcgtatcgaa
     1321 ggcccggtag tcaagaagga tggcggcgac aacattgagc gccccggtct cttcaagagt
     1381 atgcgagaga tagtgagcgg acgctctccc caagatgcgt tcgcccgaca ctacaaataa
     1441 gccgcggaag agcacggcgg cgaagagagc gctgacaagg gcggctcccg gccagcgaag
     1501 ccagaaagga cactcccggc actctccgtc gggaggaggc gtacgttgcg tccggctgta
     1561 tgccacaaga aacagcgatg taacaagtcc cgagttgata atggcctgcg tcagcgcaac
     1621 gtcgggcgca cgcaaaagag cgaagaccgt cgccataaga acccccgtca cagagaggga
     1681 gatgaacgag ctgagaaggt cgtcgagaaa tacggcaaca accgctgtta acagaataaa
     1741 gaaatagaga aaggccagaa gatgctcgtt catacgtcgt ccctcttctt ttcatcgatc
     1801 ggcctgttcg caagggttct tctgtggcat gcccttgcga tggcgtgcga cgccatcggg
     1861 ttcgaaacca ggaagaagac tgcgatgagg aagagtttca gcgcgcccgc aacgcctccg
     1921 gcatacggca tggcgcctag gagtatcagg atcgctcctc ccgaaagcgc tttcgtccct
     1981 ccgtgcagcc gggtatacac atccggaaag cggagaatag ccaccacggc ggcgaagctg
     2041 aaaaaaacgc ccgcaaagat gctgattcct ccgagaaacc gaagaagggg ggtcatttgt
     2101 gcagttctcc gtgttcaaaa tacttcgcca tcagcagcat atcggcaaag gagagcatcg
     2161 agaaggcaag cgctacgtcc aaaagaaaag gggtctcgat atacaggcta agaagcacga
     2221 gcaccatggt aaagatcacc gagagcgcat ccgcagcgac aagtctgtcc gcaggggacg
     2281 gcccggtcac tacccgtaca aaccctatca aacccgatat caccataccc cacagagcgg
     2341 ccagaatcat tcggtaaacc ccgctattcc ctcctccagc cgccggacgt cgttgcgaag
     2401 agtctcctct cccccttccg ccatgagggc atggacgagc agagaacgcc tgccgtgcag
     2461 gatatcgacg gtaagcgttc ccggggtcag ggtcacggcg tttgcgagag caactatgga
     2521 gcctttgaaa gccggagaag gcgtatatac aaatgtgccg ggggatatcg agagctccgg
     2581 acggaacgcc tggagggcga catcccatga ggctttgatc attgcaaaaa gaaagaacgg
     2641 gacaaaggag acaaaagcga cggtccgcc
//
LOCUS       DAOTIL010000331         2667 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminobacteriaceae bacterium isolate AMR_MDS_3026
            NODE_45485_length_2667_cov_3.475881, whole genome shotgun sequence.
ACCESSION   DAOTIL010000331 DAOTIL010000000
VERSION     DAOTIL010000331.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868050
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminobacteriaceae bacterium (wastewater metagenome)
  ORGANISM  Aminobacteriaceae bacterium
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae.
REFERENCE   1  (bases 1 to 2667)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2667)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:19:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,224
            CDSs (total)                      :: 3,198
            Genes (coding)                    :: 3,184
            CDSs (with protein)               :: 3,184
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2667
                     /organism="Aminobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_45485_length_2667_cov_3.475881"
                     /isolate="AMR_MDS_3026"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:3034609"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            11..505
                     /locus_tag="P5201_07580"
     CDS             11..505
                     /locus_tag="P5201_07580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012868801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HRV98432.1"
                     /translation="MICLDASAGPILVVGGGATGLRKIRTLLDAGMAVTLISPEIVPE
                     LEEIVREGRVAWERRIAERRDFEEHVFALLALPRSESETVLTLAEGTRCLVNCCGAGD
                     LGAWSLAAQFRVASSVAALPEDVPSPAESHWYTVGVASGGRSPAGSAALKRRLQHSLE
                     GDRS"
     gene            502..1494
                     /gene="hemC"
                     /locus_tag="P5201_07585"
     CDS             502..1494
                     /gene="hemC"
                     /locus_tag="P5201_07585"
                     /EC_number="2.5.1.61"
                     /inference="COORDINATES: protein motif:HMM:TIGR00212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004418 - hydroxymethylbilane synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylbilane synthase"
                     /protein_id="HRV98433.1"
                     /translation="MKLLTRGSTLALVQTERVASSLRFLGIDVDIERVSTKGDRDTIS
                     PLCSFGGSGAFSSCIEEALLRGMGDGAVHSLKDLPSCCREGLEIAAVCPRDSVEDLLL
                     CREEQNLENLPRSAVVGTSSPRRRAQLLRLRPDLSVRELRGNVATRLQKLHDGLYDAI
                     VLAHAGLERLGIRTQNTMPLPFLPAPCQGIIALEAPLNGALFPLGERITDRETHLCSI
                     AERSLLRTLGVGCHVPFAALAELHGETMLLRAEILECDGRDSVSLTFERTVGTDEEAL
                     DAGRSLGGLFRNEPRAARLLKACAVPPPPRKEERTQRAGGPSVCTAALAGGDLR"
     gene            1491..>2667
                     /gene="cobA"
                     /locus_tag="P5201_07590"
     CDS             1491..>2667
                     /gene="cobA"
                     /locus_tag="P5201_07590"
                     /EC_number="2.1.1.107"
                     /inference="COORDINATES: protein motif:HMM:NF004790.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004851 - uroporphyrin-III
                     C-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0019354 - siroheme biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen-III C-methyltransferase"
                     /protein_id="HRV98434.1"
                     /translation="MTVHLVGAGCGGPLWLTLKGKALLEGAEHIVYDSLIHPDLLQLA
                     PRACAFHPVGKRKGRPSPKQSEISALLVELGQTGKSVVRLKGGDPFVFGRGGEEALAL
                     EAAGIPWTFTPGITAALGGLGSAGIPPTHRGAADSVTLVSGHSAEGREPDADAWRALG
                     ALRGTKAIYMGASSWTKLRPLLLEGGMPPGEPCAAVAWGGWGRARLVTFSPADSPDIP
                     SPSIVALGETAGIRLSPERGPLRGLQVGLVRPSPESWATARALESLGADAYSLPLLSL
                     EELRTNGEETLIAAADWLVLTSPRGAAILLRRIDPRRISGRIAAIGEGTAAALASFGI
                     RADAVASPSTSEALAALLAARVHPGERVVFFRNKAGSPLPEEAVRSVGAFPENIAAYR
                     "
BASE COUNT          406 a          947 c          884 g          430 t
ORIGIN      
        1 agcctccctt atgatctgtc tcgacgcctc cgcaggaccg atactcgtag tcggcggcgg
       61 cgcgaccgga ctgcgcaaaa tccggacgct gctcgacgcg ggaatggctg taacgctgat
      121 ctctcccgag atcgtcccgg agctggagga gatagtgcgg gaggggcgcg tcgcctggga
      181 acggagaatc gccgaacggc gggacttcga ggagcacgtc ttcgcgcttc tcgcgctccc
      241 ccgctccgaa tctgagacgg tcctcaccct cgccgagggg acgcgctgcc tcgtgaactg
      301 ctgcggcgcc ggtgacctcg gggcgtggtc gctcgccgcc cagttccgcg ttgcatcttc
      361 cgtcgcggcg cttccggaag acgttccctc tcccgccgaa agccactggt acacagtcgg
      421 cgtcgccagc ggcggacgct ctccggccgg ctccgccgcg ctgaagcgcc gactgcagca
      481 ctctctggaa ggagaccgtt catgaaactg ctcacacgcg gaagcacact tgccctcgtc
      541 cagaccgaac gggtcgcgtc ctctcttcgt tttctcggca tcgacgtcga catcgagcgc
      601 gtctcaacca agggcgaccg ggacacgatc agccccctct gctccttcgg cggctccgga
      661 gcgttttcga gctgcatcga ggaggcgctg ctgcgcggca tgggggacgg ggcggttcac
      721 agcctgaagg acctcccctc atgctgccgc gaagggttgg agatcgccgc cgtctgcccc
      781 cgcgactccg tggaggatct cttgctctgc cgtgaggagc agaacctgga gaaccttccc
      841 cgcagcgcgg tcgtgggcac ttcgagcccc cgccgccgcg cccagctgct gcgcctccga
      901 cccgacctct ccgtaaggga gcttcggggg aacgtcgcta cccgcctcca gaagctgcac
      961 gacggactct acgacgcgat cgtcctcgcg cacgccgggc tcgagcgcct tggaatccgg
     1021 acgcagaaca ctatgccgct tccgttcctt cccgcgccct gccagggaat catcgccctc
     1081 gaagcgcccc tgaacggcgc gctcttcccg ttgggtgaac ggatcaccga cagggagacg
     1141 cacctctgtt cgatcgccga acgatccctg ctccggacgc tcggcgtcgg ctgccacgtc
     1201 cccttcgccg cgctggcgga gcttcacggg gagacgatgc tgctgcgcgc ggagatcctt
     1261 gagtgcgacg gcagggactc ggtttcgctg accttcgaga gaacggtcgg gacggacgaa
     1321 gaggcgctgg acgccgggcg ttcgctcggc ggtctcttca ggaacgaacc gcgtgcggcc
     1381 cgtctgctga aggcgtgcgc cgttccgcct ccccctagga aggaagaacg cactcaacgc
     1441 gccggggggc cttcggtctg taccgccgct ctcgccggag gcgatctccg atgaccgtcc
     1501 atcttgtcgg cgcggggtgc ggcgggccgc tctggctgac gctgaagggg aaagcgctgc
     1561 ttgaaggcgc tgagcatatc gtgtacgaca gcctgatcca ccccgacctc cttcagctcg
     1621 cccctcgggc gtgcgccttt cacccggtcg ggaagcgcaa ggggaggccg tcgccgaaac
     1681 agagcgagat cagcgcgctc ctcgtcgagt tgggacaaac ggggaagagc gtcgtccggc
     1741 tcaagggcgg cgaccccttc gtcttcggac ggggcggcga agaagcgctc gcgctcgaag
     1801 cagccggaat tccctggacc ttcactccgg gcatcacggc cgctctcggc ggactgggga
     1861 gcgcgggcat cccgcccacg cacagaggcg cggccgactc ggtcacgctc gtttccgggc
     1921 acagcgcgga gggcagagag ccggacgccg acgcatggag agcgctcggg gcgcttcgcg
     1981 gaaccaaggc aatctacatg ggagcgtcgt cgtggacgaa gctccgcccc ctgctgctgg
     2041 agggcggtat gccccccggc gaaccctgcg cagcagtcgc ctggggcggc tggggacggg
     2101 cccggctcgt caccttttct cccgcggact cgccggacat ccccagcccg agcatcgttg
     2161 cgctcggtga aacagcggga atccgcctct cgccggaacg gggaccgctg cgcggactcc
     2221 aggtcggcct cgtgcgcccc tcccccgaga gctgggcgac ggcgcgcgcg ctcgaatcgc
     2281 ttggcgccga cgcatacagt cttccgctgc tttcgctgga ggagctccgc acaaacggcg
     2341 aggaaacttt gattgcggca gccgactggc ttgtcctcac cagcccccgc ggtgcggcga
     2401 tcctgctgcg ccggatcgac ccgaggcgca tatcgggccg catcgccgcg atcggggagg
     2461 gaacggccgc tgcgcttgca tcattcggca tccgcgccga cgccgtcgct tccccctcaa
     2521 catcggaggc gctcgcggcg ctcctcgccg cccgagtcca tccgggggag cgcgtcgtct
     2581 tcttccggaa caaagccgga tcccctcttc ccgaggaagc agtccggtcg gtcggcgcgt
     2641 ttccggagaa cattgccgcc tacagga
//
LOCUS       DAOTIL010000332         2654 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminobacteriaceae bacterium isolate AMR_MDS_3026
            NODE_45776_length_2654_cov_3.384763, whole genome shotgun sequence.
ACCESSION   DAOTIL010000332 DAOTIL010000000
VERSION     DAOTIL010000332.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868050
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminobacteriaceae bacterium (wastewater metagenome)
  ORGANISM  Aminobacteriaceae bacterium
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae.
REFERENCE   1  (bases 1 to 2654)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2654)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:19:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,224
            CDSs (total)                      :: 3,198
            Genes (coding)                    :: 3,184
            CDSs (with protein)               :: 3,184
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2654
                     /organism="Aminobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_45776_length_2654_cov_3.384763"
                     /isolate="AMR_MDS_3026"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:3034609"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1040
                     /locus_tag="P5201_07595"
     CDS             <1..1040
                     /locus_tag="P5201_07595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013049248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="aldehyde ferredoxin oxidoreductase C-terminal
                     domain-containing protein"
                     /protein_id="HRV98435.1"
                     /translation="GYPYKNWQGAHMPEADSQSGETLARDFLTKRYSCYACPIGCGRV
                     TKVGDREGEGPEYESIFSFGVCCGVEPLEPIIEANYLCNEYGLDTISAGVTIAAAMEL
                     YEKGYIKKEELEGGPELKFGNAEAVTYWTKKMGLVEGFGKKLAMGSYALCEMYGHPEF
                     SMTAKKLEMPAYDGRAVQGIGLNYATSNRGGCHVRGYTTSPEVLGLPQKLDPAEYQSK
                     PTWVKIFQDLTAAIDSSGMCLFTSFALGADDYAAFLKAATGFEYDGAEALKAGERIWN
                     IERLFNLREGMDTAEDTLPARLLNEPIPEGPAKGMVSHLPEMLPEYYKLRGWDSKGIP
                     TTGKLEELGLQ"
     gene            1045..1317
                     /locus_tag="P5201_07600"
     CDS             1045..1317
                     /locus_tag="P5201_07600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012869227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MoaD/ThiS family protein"
                     /protein_id="HRV98436.1"
                     /translation="MIKVLFFATIRDYTNEKETTAEGASSVEGLLRLLCARYGPEFEA
                     EVFDGGKISDRVIVLVNGKHIAHTGGPLTPVKDGDTIAVFPIIGGG"
     gene            1350..>2654
                     /locus_tag="P5201_07605"
     CDS             1350..>2654
                     /locus_tag="P5201_07605"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="S9 family peptidase"
                     /protein_id="HRV98437.1"
                     /translation="MKRTVELTDLLRYHFLSAPTFSPDGTKIAFLLHKADYENNAYSS
                     NIWMHDLLTGSLRQLTASGKEKFFAWKHDNRTLVFASGRNDAPKTETVFYTLDICGGE
                     AVPLRTVKRALSSFTVLSDGTFLALGVFEQAFDNPYDAEMLFFDQVPFCSNGKGFTGQ
                     KKKSLCLYTPEGEEKRLTPEDMDVENYVVSPDGEKLLAWGPVFKDVKGQFSALLQIDR
                     KSGATTTLLPGTDFACKWAGWLDGKAVVTGTDFKKYGVNENVKFFFVENGETACITPE
                     LDKGLRNSVGSDCRYGSTDLNLAFFTDGPAAWFVSTDNYRSHLHAVTASGAMEQITKE
                     LFSVDDWRVSGGKAAVVGMKGLQLQELYLVDREREIQLTHFNDWLLEECELSAPEHVT
                     FDNGTGEQLDGWYMKPVGFEEGKKYPAILNIHGGPKFTYGDVF"
BASE COUNT          587 a          829 c          788 g          450 t
ORIGIN      
        1 gcggctaccc ctacaagaac tggcagggcg cccacatgcc cgaagccgac tcacagtccg
       61 gcgagacgct cgccagggat ttcctcacca agagatactc ctgctacgca tgccccatcg
      121 gctgcggacg cgtgaccaag gtcggcgacc gtgagggcga gggccccgag tacgagagca
      181 tcttctcctt cggcgtctgc tgcggcgtgg agcctctgga gccgatcatc gaagcgaact
      241 atctctgcaa cgagtacggc ctcgacacga tcagcgcggg cgtgaccatc gcggcggcca
      301 tggagctgta cgagaagggg tacatcaaga aggaagagct ggagggcggc cccgaactga
      361 agttcggcaa cgccgaagcc gttacctact ggacgaaaaa gatgggactc gtcgagggat
      421 tcggcaagaa gctcgccatg ggttcctacg ccctctgcga gatgtacggg caccccgagt
      481 tctccatgac ggccaagaag cttgagatgc ccgcgtacga cggacgcgcc gtccagggga
      541 tcggcctgaa ctacgccacc tccaaccgcg gcggctgcca cgtccgcggc tacacgacct
      601 cgcccgaagt gctcggcctg cctcagaagc tcgacccggc ggagtatcag tcaaagccga
      661 cgtgggtcaa gatcttccag gatctcactg cggcgatcga ctcctccggc atgtgcctct
      721 tcacctcctt cgccctgggc gccgacgact acgccgcgtt cctgaaggcc gccaccggct
      781 tcgaatacga cggcgccgaa gccctgaagg caggcgagcg catctggaat atcgagcgtc
      841 tgttcaacct tcgcgagggt atggatacag cagaggacac gctccccgcg cgccttctga
      901 acgaaccgat ccctgaaggc ccggccaagg gcatggtgag ccacctgccg gaaatgcttc
      961 cggaatacta caagcttcgc ggctgggact ccaaaggcat ccccaccact ggaaaactgg
     1021 aggaactcgg gttacaataa gcgcatgata aaagtactgt tcttcgcgac tatccgggac
     1081 tacacgaacg agaaggagac aacggcggag ggagcctctt cagtagaggg gctcctccgt
     1141 ctcctttgcg cgcgctacgg tcccgagttc gaagccgagg ttttcgatgg gggaaaaatt
     1201 tccgaccggg tgatcgtact ggtgaacggc aaacacatcg cccacaccgg aggaccgctc
     1261 accccggtca aggacggcga cacgatcgcc gtcttcccta tcatcggcgg aggatagccg
     1321 cacacttcca ggcaaagggg accagaaaat tgaaaagaac cgtagaactc accgatctgc
     1381 ttcgatacca ctttctctcc gcaccgacgt tctctcccga cggcacgaaa atagcatttc
     1441 tcctccacaa ggccgactac gaaaacaacg cctacagctc gaacatctgg atgcacgacc
     1501 tgcttacggg aagccttcgg cagctcacag cctccggcaa ggagaagttt ttcgcgtgga
     1561 agcacgacaa caggacgctc gtcttcgcca gcggacgcaa cgacgccccc aagacggaga
     1621 cggtgttcta cacgctcgac atctgcggag gcgaggccgt tccgcttcgc accgtgaagc
     1681 gcgcgctctc ctcgtttacc gtcctttcgg acggcacttt ccttgcgctc ggcgtcttcg
     1741 agcaggcgtt cgacaaccct tacgacgcgg aaatgctctt cttcgaccag gtacctttct
     1801 gctccaacgg aaaggggttc acggggcaga agaaaaagtc gctctgcctc tacactccgg
     1861 agggcgagga gaaacgcctc acccccgagg atatggacgt ggaaaattac gtcgtctctc
     1921 cggacgggga gaagctgctt gcttgggggc cggtcttcaa agacgtcaag ggccagttca
     1981 gcgcactgct tcagatcgac cggaagagcg gcgctacaac cacgctcctt cccggaaccg
     2041 acttcgcgtg caagtgggct gggtggctcg atggaaaggc ggtcgtcacc ggaaccgact
     2101 tcaagaagta cggagtgaac gagaacgtga agtttttctt cgttgagaac ggagaaacgg
     2161 cctgcatcac ccccgaactt gacaaggggc tccggaactc tgtgggaagc gactgccgct
     2221 acggctcgac cgacctgaac ctcgccttct tcacggacgg tccggcggcc tggttcgtct
     2281 ccacggacaa ctaccgctcg catctgcacg cagtcacagc ctccggggcg atggagcaga
     2341 tcacgaagga gctcttcagc gtggacgact ggagggtttc cggcggcaaa gcggccgtcg
     2401 tggggatgaa ggggctccag cttcaggagc tctacctcgt ggacagggaa cgcgagattc
     2461 agctcaccca cttcaacgac tggctgctgg aggagtgcga actgtcggcg ccggaacacg
     2521 tgacgttcga caacggaacg ggcgaacagc tcgacgggtg gtacatgaag ccggtcggct
     2581 tcgaagaagg aaagaagtac cccgccatcc tgaacatcca cggcgggccc aagttcacct
     2641 acggcgacgt gttc
//
LOCUS       DAOTIL010000333         2648 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminobacteriaceae bacterium isolate AMR_MDS_3026
            NODE_45916_length_2648_cov_4.183571, whole genome shotgun sequence.
ACCESSION   DAOTIL010000333 DAOTIL010000000
VERSION     DAOTIL010000333.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868050
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminobacteriaceae bacterium (wastewater metagenome)
  ORGANISM  Aminobacteriaceae bacterium
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae.
REFERENCE   1  (bases 1 to 2648)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2648)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:19:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,224
            CDSs (total)                      :: 3,198
            Genes (coding)                    :: 3,184
            CDSs (with protein)               :: 3,184
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2648
                     /organism="Aminobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_45916_length_2648_cov_4.183571"
                     /isolate="AMR_MDS_3026"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:3034609"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1006)
                     /gene="hflK"
                     /locus_tag="P5201_07610"
     CDS             complement(<1..1006)
                     /gene="hflK"
                     /locus_tag="P5201_07610"
                     /inference="COORDINATES: protein motif:HMM:TIGR01933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0045861 - negative regulation of
                     proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsH protease activity modulator HflK"
                     /protein_id="HRV98438.1"
                     /translation="MRNIFDEIEELYEKTRRKERNFDGEEYGGGGGNEKPRRRFSFSF
                     GFLKLLLPVLLLLIVAWQGFYIVPAGSRGIVFRLGKVSHVANQGMNFKLPVVDQVTIV
                     NTEQIQRFEYGYRTVNPGPPPTYSDKPEESKMLTGDNKIVEIDWVLQYQAGTPEEYVV
                     NLPGDQWAREKLIRDVAESTLREVIASRALDDVLTTEKEASQTEAKMLIQGKLNALKT
                     GVFIVAVQFQDVTPPKAVQAAFSEINTARSEQERLILEADKYSNEIISKAQGESERIL
                     NEAEAYKFRRISLAEGEASRIRSLNAAYKENPDLVLNNLWLESMVKVWPKLKVFIVDG
                     G"
     gene            complement(1003..1863)
                     /locus_tag="P5201_07615"
     CDS             complement(1003..1863)
                     /locus_tag="P5201_07615"
                     /inference="COORDINATES: protein motif:HMM:NF016330.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SWIM zinc finger family protein"
                     /protein_id="HRV98439.1"
                     /translation="MSGWWYNDYTPAPPRAVRGGIKASGKGGFAKKWWGKRWLDVLES
                     FGIGARLSRGRAYAKKGQVADLSIGTGLIRASVQGTRVKPYTITIQLAPYSAAQWDEI
                     FGAIAEHPVVAARLLAGEMPEEMEELCAKRKLPLFPKRQGDMVTECSCPDWSNPCKHI
                     AAVFFVMAEAFDGDPFLLLRLRGMDRDVFISRIAPGASEEEHEEEIGAEPLPAEPSRF
                     WGPEGEIPEIRIPASLPKVHGALPGRLGPLPFWRGKETFPETMAALYESAACRAEEIY
                     RTLEENGGIR"
     gene            complement(1860..>2648)
                     /locus_tag="P5201_07620"
     CDS             complement(1860..>2648)
                     /locus_tag="P5201_07620"
                     /inference="COORDINATES: protein motif:HMM:NF012493.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helicase-related protein"
                     /protein_id="HRV98440.1"
                     /translation="RLKSDRSIISDLPDKIETKSYCPLTKEQASLYAAVLADIDERLT
                     NSEGIERKGLVLAALTRLKQVCNHPAHFAGDGSALTGRSGKLERLMEMLEEARESGGR
                     TLVFTQFAEMGELMASHIREYFAEEIFLLHGGVPKKKRDEMVARFQEDAAAPHVFILS
                     LKAGGSGINLTRADRVIHYDRWWNPAVENQATDRAYRIGQTKNVQVYKFLVAGTLEEK
                     IDALIERKSAIAGTIIGAGENWLTELSDGELRDLLALEKEAVVE"
BASE COUNT          446 a          878 c          750 g          574 t
ORIGIN      
        1 ctccgccatc aacgatgaaa accttcagct tcggccagac cttgaccatg ctctcaagcc
       61 agagattgtt gagcaccagg tcgggattct ccttgtacgc cgcgttcagc gaacggatgc
      121 gcgaggcttc gccctccgcg agcgatatcc ggcggaattt gtatgcttca gcctcgttca
      181 ggatacgttc cgattcgccc tgcgccttcg agataatctc gttggagtac ttgtctgcct
      241 ccaggatcag ccgctcctgc tcggagcgcg ccgtgttgat ttcgctgaac gcggcctgga
      301 ccgccttcgg gggggttacg tcctggaact gcaccgcgac gatgaagacc ccggtcttca
      361 gcgcattcag cttcccctgg atgagcatct tggcctccgt ctgcgaggcc tccttctccg
      421 tggtgagcac gtcgtcgagc gcacgtgagg cgataacctc gcgcagagtg gattcggcaa
      481 cgtcgcggat cagcttctcc cgggcccact ggtctccggg aaggttcacc acgtactcct
      541 ccggcgttcc cgcctggtac tggagtaccc aatcgatctc gacgatcttg ttgtcgccgg
      601 tgagcatctt cgactcctcg ggcttgtccg agtacgtcgg gggaggtccg gggttcaccg
      661 tccggtagcc gtactcgaag cgctggatct gttccgtgtt cactatagtg acctggtcga
      721 cgacgggaag tttgaagttc attccctggt tcgccacgtg ggagactttg cccaagcgga
      781 agacgatacc ccgcgaccct gccgggacga tatagaaccc ctgccaagcg acgataagca
      841 gaagcagtac ggggagcaga agtttcagga agccgaagct gaaggagaaa cggcgccggg
      901 gtttttcgtt ccccccgcct cccccgtact cctcgccgtc gaagtttctt tccttgcgtc
      961 tcgtcttttc gtaaagctcc tcgatttcat cgaaaatatt tctcatctga tacctccgtt
     1021 ttcttcaagt gtgcgataga tctcttccgc cctgcacgcc gctgattcgt agagagccgc
     1081 catcgtctcc gggaaggtct ccttacctcg ccagaagggg agcggtccaa ggcgtccggg
     1141 gagcgccccg tgcactttcg ggagcgatgc gggaatcctg atttcgggaa tctcgccttc
     1201 cggcccccag aagcgcgacg gttccgccgg aagtggttcg gctccgatct cctcctcgtg
     1261 ttcctcctcc gatgcgcccg gggcgatgcg ggagataaag acgtcccgat ccattccgcg
     1321 cagcctgagc agcaggaagg gatctccgtc gaacgcctcc gccatcacga aaaagactgc
     1381 ggctatgtgc ttgcacgggt tggaccagtc ggggcaggag cattcggtga ccatgtcccc
     1441 ctgtcgtttc gggaaaagag gaagctttct cttcgcacac agctcctcca tctcttcggg
     1501 catctccccg gccaaaagac gggccgcgac gaccggatgt tccgcgattg cgccgaagat
     1561 ctcgtcccac tgcgccgcgg agtagggggc aagttggata gtgatcgtat acggtttgac
     1621 cctcgtcccc tggacggatg cccgtatcag ccccgtcccg atggagaggt cggccacctg
     1681 ccccttcttc gcgtaggcgc gtccacgcga gagacgggcg ccgatgccga aggattcgag
     1741 cacatcgagc caccgttttc cccaccactt cttcgcgaag ccgcccttgc ccgaagcctt
     1801 gattccgccg cgcaccgccc gcggcggcgc gggcgtgtag tcgttgtacc accatccgct
     1861 cattccacca cagcctcctt ctccagtgcg agcaggtcgc gcagctctcc gtccgagagt
     1921 tccgtcagcc agttctcccc cgcgcctata attgtacccg caatcgcgga tttgcgttcg
     1981 atgagcgcgt cgattttctc ctccagcgtc ccggcgacca gaaatttgta cacctgcacg
     2041 tttttcgtct gcccgatccg gtaggctctg tcggtagcct ggttctccac ggccggattc
     2101 caccaccggt cgtagtggat caccctgtcc gcccgcgtca ggttgatccc cgaaccgccc
     2161 gccttcagag agaggatgaa gacgtgcggg gcggcggcgt cctcctgaaa gcgcgcgacc
     2221 atctcgtcgc gcttcttttt cggcacgccg ccgtgaagca ggaaaatctc ttcggcgaag
     2281 tactcccgga tatgcgacgc catcaattcg cccatttccg cgaactgggt gaagacgagc
     2341 gtgcgtccgc cgctctccct cgcttcctcc agcatctcca tcagccgctc caacttgccg
     2401 gaacgccccg tcagcgccga tccgtccccg gcgaagtgcg ccggatggtt gcacacctgc
     2461 ttcagccgtg tcagcgccgc gaggacgagc ccctttcttt cgatgccctc tgaattggtc
     2521 agacgctcgt cgatgtccgc gaggaccgcc gcatacagag acgcctgctc cttggtgagc
     2581 gggcagtagc tcttcgtctc gatcttgtcc ggcaggtcgg agatgatgct cctgtccgac
     2641 ttcagtct
//
LOCUS       DAOTIL010000334         2646 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminobacteriaceae bacterium isolate AMR_MDS_3026
            NODE_45963_length_2646_cov_3.455809, whole genome shotgun sequence.
ACCESSION   DAOTIL010000334 DAOTIL010000000
VERSION     DAOTIL010000334.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868050
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminobacteriaceae bacterium (wastewater metagenome)
  ORGANISM  Aminobacteriaceae bacterium
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae.
REFERENCE   1  (bases 1 to 2646)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2646)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:19:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,224
            CDSs (total)                      :: 3,198
            Genes (coding)                    :: 3,184
            CDSs (with protein)               :: 3,184
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2646
                     /organism="Aminobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_45963_length_2646_cov_3.455809"
                     /isolate="AMR_MDS_3026"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:3034609"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..603)
                     /locus_tag="P5201_07625"
     CDS             complement(<1..603)
                     /locus_tag="P5201_07625"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRV98441.1"
                     /translation="MDAGRFTALIDSFLRSSGWNTVAAGRAINEEMTGLRIFDAPLTG
                     VASADDPAFRDLRRESVVGPHFLLPEEWLPGARAVVSFFLPFSENVRRANRADRDEPA
                     PEWLHGRIEGQECIDRLSAFLVERLLEEGFDAVAPSVDERFRSCTTTNTVFLDGDGAP
                     LPVSFNSNWSERHVAYVCGLGSFGLSKGLITPKGVAGRFGS"
     gene            913..1638
                     /locus_tag="P5201_07630"
     CDS             913..1638
                     /locus_tag="P5201_07630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014162436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HRV98442.1"
                     /translation="MAMNAEYTPLFRARGITKIYHPGTETEVRALDGVDLDLYEGELA
                     VLLGASGSGKSTLLNILGGLDVPTDGTLSYRDEELTGAGERELTLFRRNVAGFVFQFY
                     NLIPSLTARENVALITEIAPDPMEPEEALGLVGLSDRMDHFPSQLSGGQQQRVAIARA
                     IAKRPEVLLCDEPTGALDVHTGITVLEAIERVNRELGTLAVIITHNAAIAAMADRVLS
                     LSAGRILSIHSNEKRAPVNTLSW"
     gene            1651..>2646
                     /locus_tag="P5201_07635"
     CDS             1651..>2646
                     /locus_tag="P5201_07635"
                     /inference="COORDINATES: protein motif:HMM:NF024116.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HRV98443.1"
                     /translation="MLDLKLLRDIRGMKGQMTAIALVMACGLTLMIMSRGLLLSLETT
                     KNSYYTESRFADLFCDLKRAPLFLEPALAMIDGVAQVETSVRGAIILDIPGMKEPADG
                     FVHSLPEGRPRRLNRIHLRSGRLPEAGNRDEILVSDSFAKAHGFRPGDTIDATVYGVR
                     RSFRICGVAISPEYVYEIRPGGMMPDPERFGVFWMSERELSRALGLEGAFNSVAFRLA
                     PGADAGMVKADIDRLLEPYGGLSAYDRERHVSAKLMEDEVAQLRVFSVAFPVVFLSIA
                     AFMVSAALTRLVKLQREQIAQMKAFGYSGFSVASHYVKFAFVPVVAATLLSGTLGM"
BASE COUNT          479 a          835 c          833 g          499 t
ORIGIN      
        1 gctgccgaat ctccccgcga cgcctttcgg cgtgatcagc cccttggaga gcccgaacga
       61 ccccagtccg cagacatagg cgacatgccg ctccgaccag ttgctgttga acgataccgg
      121 cagaggagcg ccgtcgccgt ccagaaacac cgtgttcgtc gttgtgcagg agcggaagcg
      181 ttcgtcgacg gacggagcga ccgcgtcgaa tccttcttcc agaagacgct cgacaaggaa
      241 cgcggaaaga cggtcgatgc actcctgccc ttcgatcctg ccgtgcagcc actcgggggc
      301 cggttcgtcc ctgtcggcgc ggtttgcgcg gcgcacgttc tccgagaacg ggagaaagaa
      361 cgaaaccacg gcgcgcgcgc cgggaagcca ctcctccggc aaaaggaagt gcggccccac
      421 aacgctctcc ctgcgaagat cccggaaggc ggggtcatcc gccgaggcga cgcccgtaag
      481 cggcgcgtcg aagatccgga gtccggtcat ctcttcgttg atggcgcgcc cggctgcgac
      541 cgtgttccaa ccggaactcc gcagaaaact gtcgatcagc gcagtaaacc gtcctgcatc
      601 catagagatc tccgtccttt ccatatcacg gcggggagct ttgaatacaa cgcgctcacc
      661 gcctcgttca ccgcgggaat ccgcgtcttc cctccctcgt ttgcagcctc tatactacgt
      721 gaaaaggccc tgttttccaa gatctgtcgg tccatgccag tctttttccg caaagcgtcc
      781 gatgaaaaat ctgcggaaat cttccgctat tgtttgactg atgcagccca ttgggatata
      841 acgttgcagg aacttcccgc tgccgatact gcgtgcaggg ggtgcagcgc gtgtttttca
      901 ggaggaacgt tgatggcgat gaatgcggag tacacccccc tctttcgagc gcgggggatc
      961 acgaagattt accatccggg gacggagacg gaggtgcgcg ccctcgacgg cgtcgatctc
     1021 gatctctacg agggggagct ggccgtgctc ctcggcgcct cggggagcgg gaagtccacg
     1081 ctgctcaaca tcctcggcgg gctggacgtc ccgacggacg ggacgctctc gtacagggac
     1141 gaggagctca ccggcgcggg cgagcgggag ctcacactct tccgcaggaa tgtcgcgggg
     1201 ttcgtcttcc agttctacaa cctcattccg agccttaccg cgcgcgagaa cgtggcgctc
     1261 atcaccgaga tcgcgcccga tcccatggag ccggaagagg cgctcggtct cgtcggtctt
     1321 tcggacagga tggaccactt cccctcgcaa ctctccggag ggcagcagca gcgggtcgcc
     1381 atcgcgcgcg ccatcgcgaa gcgtccggag gtgctcctct gcgacgagcc gaccggggcg
     1441 ctcgacgtgc acaccggcat cacagtgctg gaggcgatcg agcgggtgaa cagagagctg
     1501 ggaacgctcg ccgtgatcat cacgcacaac gcggcgatcg cggcgatggc cgaccgggtg
     1561 ctctctctct cggcggggcg tattctctcc attcattcga acgagaagcg cgcgccggtg
     1621 aacacgctct cctggtgatg tgtaatgagg atgcttgatc tcaaactgct cagggatatc
     1681 cgcggaatga aggggcagat gaccgccatc gccctggtga tggcgtgcgg tctgaccctg
     1741 atgatcatgt cgcgcggact gcttctcagc cttgaaacga cgaagaacag ctactacacg
     1801 gaatcgcgct tcgcggatct cttctgcgat ctcaagcgcg ctcccctgtt cctcgagccc
     1861 gccctcgcga tgatcgacgg cgtggcgcag gtggaaacga gcgtcagggg agcgatcatc
     1921 ctcgatattc ccggaatgaa ggagcctgcg gacggcttcg tccactcgct tccggaggga
     1981 cgcccccggc ggctcaaccg cattcatctg cgctcggggc gtcttcccga ggcgggaaac
     2041 agggacgaga ttctcgtcag cgactccttt gccaaagccc acggcttccg ccccggagat
     2101 acgatcgacg ccaccgtcta cggcgtgcgg cgcagcttcc gtatctgcgg cgtcgccatc
     2161 tcccccgagt atgtctacga gatcagaccg ggaggcatga tgcccgaccc ggagcgcttc
     2221 ggggttttct ggatgagcga acgcgagctc tcgcgggcgt tggggcttga gggcgccttc
     2281 aacagcgttg ccttccggct tgcgccggga gcggacgccg ggatggtgaa agcggacata
     2341 gacaggcttc ttgagccgta cggagggctg tctgcgtacg accgtgaacg gcacgtctcg
     2401 gcaaagctga tggaggacga ggttgcccag ctcagagtgt tttctgtggc gtttcccgtc
     2461 gttttcctca gcatcgccgc gtttatggtg agcgcggcgc tgacccgtct cgtcaagctg
     2521 caacgcgagc agatagcgca gatgaaggcg ttcggatact ccggcttttc cgtggcctcg
     2581 cattacgtga agttcgcctt tgtcccggtg gtcgccgcaa cgctgctcag cggcacgctc
     2641 ggcatg
//
LOCUS       DAOTIL010000335         2636 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminobacteriaceae bacterium isolate AMR_MDS_3026
            NODE_46189_length_2636_cov_4.037970, whole genome shotgun sequence.
ACCESSION   DAOTIL010000335 DAOTIL010000000
VERSION     DAOTIL010000335.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868050
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminobacteriaceae bacterium (wastewater metagenome)
  ORGANISM  Aminobacteriaceae bacterium
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae.
REFERENCE   1  (bases 1 to 2636)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2636)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:19:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,224
            CDSs (total)                      :: 3,198
            Genes (coding)                    :: 3,184
            CDSs (with protein)               :: 3,184
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2636
                     /organism="Aminobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_46189_length_2636_cov_4.037970"
                     /isolate="AMR_MDS_3026"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:3034609"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..633)
                     /locus_tag="P5201_07640"
     CDS             complement(<1..633)
                     /locus_tag="P5201_07640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006301370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase"
                     /protein_id="HRV98444.1"
                     /translation="MKRSLWNKIGLTLLALLVLAGAACAAGVNNEWGGAKAKYVFMFI
                     GDGMGLQQVNAAEVYNGSIDRTGGEPAIKKLALSGLPVQGMITTYSTNSFITDSAPAA
                     TSLASGYKTDNGVIGMDPAKKQKFTTVAQMVRAKGMKVGVLTSVSLNHATPAAFYASV
                     PSRNDYYGIGEQLIDSGFDFFAGGGLHQSTGKEKDRKDLYEIAKEKGYQVL"
     gene            complement(828..1763)
                     /locus_tag="P5201_07645"
     CDS             complement(828..1763)
                     /locus_tag="P5201_07645"
                     /inference="COORDINATES: protein motif:HMM:TIGR01297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation diffusion facilitator family transporter"
                     /protein_id="HRV98445.1"
                     /translation="MKRSIVSDETRNKEAKKATWTGFAVNALLTFFKLFAGFRGGSAA
                     MIADGVHSLSDFFTDIVVLVGFKFTEKPADDEHNYGHGKYETLATVVISLALLLVGYK
                     IMTSGIVDIYEVVFKGKVLEQPEMIAFVAAVFSIASKELLFRYTRRVGEKIQSSAVIA
                     NGWHHRSDAFSSIGTMVGIGGAIFLGRNWAFLDPLASLIVSVFIFKVSFEILMPAVNE
                     LLESSLQDEEIAYIKKIIEASSHIQNYHHLRTRRIGAKVAIEVHFVFEKTMSIREAHS
                     YATEVEKKIQEYFGASSLITTHLEPSRDAQEATRA"
     gene            complement(2014..>2636)
                     /locus_tag="P5201_07650"
     CDS             complement(2014..>2636)
                     /locus_tag="P5201_07650"
                     /EC_number="3.1.3.1"
                     /inference="COORDINATES: protein motif:HMM:NF012470.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="alkaline phosphatase"
                     /protein_id="HRV98446.1"
                     /translation="KEALAFARARPNDTLIVVLADHETGGMRVDAERAAANLFPALSA
                     QRESSESFDEKLERFRKGTARMFERMAPLLAESFGLHATSSAGRAALDARAKKGDGAA
                     RTALLSALSGEELDELRTAFAQSMRAKEQRKKDRDYLRRYGPYEPLSVTATRILNRKA
                     GVEWSSFAHTGADVPVFAQGAGAARFAGEYDNTNVAEKILSVLGKR"
BASE COUNT          515 a          807 c          739 g          575 t
ORIGIN      
        1 gagaacctgg tagcccttct ccttcgcgat ttcgtacaga tctttgcggt ccttttcctt
       61 gccggtgctc tggtgcagcc cgccgccggc gaagaaatcg aacccgctgt cgatgagctg
      121 ctctccgatg ccgtagtagt cgttccggga cgggacggag gcgtaaaagg ctgcgggggt
      181 ggcgtggttc agggagacgg aggtgaggac gccgactttc attcccttgg cgcggaccat
      241 ctgggcgacg gtggtgaact tctgtttctt ggcggggtcc atgccgatga cgccgttgtc
      301 cgtcttgtag ccggaggcca gcgaggtcgc cgcgggggcc gagtcggtga tgaaggagtt
      361 ggtggagtag gtggtgatca tcccctgcac cggaaggccg ctcaaggcga gcttcttgat
      421 cgcgggctcg ccgcccgtcc tgtcgatgga gccgttgtag acctccgccg cgttgacctg
      481 ctgtaacccc ataccgtcgc cgataaacat gaacacgtac ttcgccttcg cgccgcccca
      541 ttcgttgttg acccccgcag cacaggccgc ccctgccagc accagcagcg cgagcagcgt
      601 caatccgatc ttgttccaca gtgaacgctt catatttttt accctcctcg aagaatttga
      661 gccaattatc ttcaaggagg gtaacggaag aagcgaagaa gtgtatcggg agggtaaaac
      721 tcgctcggcg cgcggtcgcc gcgcctcctc cggcctcagt atcccgcgga gagcgcaccg
      781 gaaagatgtc cgtatacggc gcgtccgtct ccgcagcccc ttcccgctta tgcccgcgtc
      841 gcttcctgcg cgtcgcggct cggctccaga tgcgtcgtta taagactgga agcgccgaaa
      901 tactcttgta tctttttttc cacttcggtt gcataagaat gcgcctcgcg aatcgacatg
      961 gttttttcaa aaacgaagtg aacttcaatg gcaaccttcg ccccgatccg tctcgttctc
     1021 aggtgatggt aattttgaat atgcgaagag gcctcgatga tttttttgat gtatgcgatc
     1081 tcttcatcct gaagagagga ctccagcagt tcattgacgg ccggcatcaa aatctcgaaa
     1141 gacaccttga aaataaacac gctgacgatc agagacgcca gaggatcaag aaaggcccag
     1201 tttcgaccta aaaatatcgc tcctccgatt ccgaccatcg ttcctatgga ggaaaacgca
     1261 tccgaccggt ggtgccatcc gttggcgatc accgcggacg attggatttt ctctccaaca
     1321 cgccgggtat accggaacaa cagctctttg cttgctatcg agaacaccgc cgcgacgaac
     1381 gcgatcattt ccggctgctc aagtactttc cctttgaaca caacttcata aatatccaca
     1441 atgccggacg tcattatttt atacccgacc aacagcagcg ccagactgat gacgaccgtc
     1501 gcaagcgttt catattttcc atgtccgtag ttgtgttcat catcggcggg tttctccgta
     1561 aatttaaaac cgaccagcac cacgatgtcc gtaaaaaaat ccgacagaga gtgcaccccg
     1621 tccgcaatca tcgcggcgct tccgcctctg aatccggcga agagtttaaa gaacgtcaac
     1681 aatgcgttca cggcaaagcc cgtccaggtc gcctttttgg cctctttgtt tctcgtttcg
     1741 tccgagacga tactccgctt catagcgata cgctccttcg ttctctacga ataaaaaact
     1801 gtggaaccat gaatgacgat cacagtccca caggtcgtac ctgctgcctt tcggcccggc
     1861 tgctgcacag tatgcaacac ctgatgccgt tttagtatat aaagagtgaa tagacttgtc
     1921 aatgtgttgc gagccgtcgt atgcgttccg gttcacaagg agccccggag aaagtccccg
     1981 tccgcccctc ggagtttcgg attgccgctt cgtctagcgt ttgccgagaa cggacaggat
     2041 cttttcggcg acgtttgtat tgtcgtactc cccggcgaag cgtgccgcgc ccgcgccctg
     2101 tgcgaagacg gggacgtcgg cccctgtgtg ggcgaaggaa gaccactcta cccccgcctt
     2161 ccggttcaga atgcgcgtag cggtcacgga aagcggttcg taggggccgt agcggcgcag
     2221 atagtccctg tccttcttcc gctgctcctt ggcccgcatg ctctgcgcga aggccgtccg
     2281 cagctcgtcc agctcttcgc ccgagagcgc ggacagaagc gccgtccgcg cggccccgtc
     2341 gcccttcttc gccctggcat caagcgcggc gcgcccggcg gaggaggtgg cgtggaggcc
     2401 gaaggactcc gcgagcagcg gcgccatgcg ctcgaacatt cgcgccgtcc ccttgcggaa
     2461 gcgctcgagc ttctcgtcga aggactccga agactccctc tgcgccgaga gcgccgggaa
     2521 gaggttcgcc gccgcgcgct ccgcgtcgac gcgcatgccg cccgtctcgt ggtcggcgag
     2581 gacgacgatc agcgtatcgt tcgggcgggc gcgggcgaag gccagcgcct ccttca
//
LOCUS       DAOTIL010000336         2635 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminobacteriaceae bacterium isolate AMR_MDS_3026
            NODE_46218_length_2635_cov_3.898062, whole genome shotgun sequence.
ACCESSION   DAOTIL010000336 DAOTIL010000000
VERSION     DAOTIL010000336.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868050
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminobacteriaceae bacterium (wastewater metagenome)
  ORGANISM  Aminobacteriaceae bacterium
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae.
REFERENCE   1  (bases 1 to 2635)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2635)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:19:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,224
            CDSs (total)                      :: 3,198
            Genes (coding)                    :: 3,184
            CDSs (with protein)               :: 3,184
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2635
                     /organism="Aminobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_46218_length_2635_cov_3.898062"
                     /isolate="AMR_MDS_3026"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:3034609"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            245..1078
                     /locus_tag="P5201_07655"
     CDS             245..1078
                     /locus_tag="P5201_07655"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRV98447.1"
                     /translation="MDRSREVVFLEREMEDLTVYIDGDKQIPDILARVPTQSGREVWL
                     VLHLEIQGPGGGDLPERMFYYNSTLRVKYLKEKGDVADAVSCAILTAKRPSLEEERYL
                     RESYGNRMFYEYPTLKLCELGADELEESENPFDWALCAGKRALESGRNERLKLVYLKD
                     MIVKLDYKEWTHDEKLLLLRLIDSLLHPKSPELRAEYGEFREELRKEGKAMLLSIVEE
                     RAQEEGREIGKSEGILIGREETKREAALRMLRKGISVSLAAECVDLPEEEVARLAEEA
                     R"
     gene            1911..>2635
                     /locus_tag="P5201_07660"
     CDS             1911..>2635
                     /locus_tag="P5201_07660"
                     /inference="COORDINATES: protein motif:HMM:NF012376.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HRV98448.1"
                     /translation="MPEVFVTGDKHGEIEVKDLSLRRFPAGNVLTKRDFVVILGDFGL
                     LWGNPPRDSERYWLNWLSEKKWTTLVIDGNHENFDLFDSLPQEERWGGPVGVIAPDVY
                     HLKRGYVYEIAGRKVFVFGGGESTDKDGRIPGKSWWPRELPSYAETERGFETLERAGW
                     KVDWVWTHAAPRRAFDELMENHYAVAHLQREKMDDPVSRYLDELAGRLEFSLWCFGHY
                     HLQSRPFEAGANGLFRAEYREVRP"
BASE COUNT          606 a          683 c          865 g          481 t
ORIGIN      
        1 ctccgccacc ccttgcgatg cggagaaaag ccgaaggagg gcgagcaaaa tggccgccga
       61 tatggaacga acgaatccca gtctcgggtc ctagggcccc gccgcgacgg aaagcgccgc
      121 gagcgcggct acggaggacg cggagaagag ggagcggagg agaatcgacg aacactggcg
      181 cgcggtcatc aagcacttct tctacctcgt attgtgcaga atcatgaccg cgctggccga
      241 ggacatggac cgatcgcgcg aggtggtctt tctggagagg gaaatggagg atctgaccgt
      301 ctacatcgac ggggacaagc agatccccga tattctggcc cgcgttccga cacagtcggg
      361 gcgggaggtc tggctggtcc tccacttgga gatacaaggc cccggaggcg gcgaccttcc
      421 ggagagaatg ttctattata attcgacgct tcgggtgaag tacctgaagg aaaagggcga
      481 tgttgccgac gcggtctcct gcgccatcct gacggcgaag cgcccctcct tggaggagga
      541 gcgttatctg cgtgagagct acggaaacag gatgttctac gagtacccga cgctcaagct
      601 gtgcgagctt ggcgccgacg agcttgagga gagcgagaat ccgttcgact gggcgctctg
      661 cgccgggaag cgggcgctgg agagcggacg gaacgagcgg ctcaagctgg tctatctgaa
      721 ggacatgatc gtgaagctcg actacaagga gtggacgcat gacgagaagc tgctgctcct
      781 ccgtctgatc gacagcctgc tgcacccgaa gagccccgag ctgagggcgg agtatggaga
      841 attcagagag gaactgagga aggaggggaa ggccatgctg ttgagcatag tcgaggaaag
      901 agcgcaggag gaaggcaggg agataggcaa gagcgaggga atcctgatcg gcagggagga
      961 gacaaagcgc gaggccgctc tcaggatgct gcgcaagggg ataagcgtct ctctggcggc
     1021 ggagtgcgtc gatcttcccg aggaggaggt tgctcgcctc gccgaagagg cgcgctgatc
     1081 cgttcgactc gcgacgggag gtgctcgcaa acgagctccc cccgtctaaa ttcattgcgg
     1141 gacgagtgct ccggactcgc cgggagactc cggagcgcgt tccggcttgg caagtatcgg
     1201 cgcgtgatcg tcaataagtt cttcttccag actccggacg ggagaggaga acgagactct
     1261 tccgaatgga tgaaacgaag gacgtcggtt aaacggacca tcttctcgaa tgtatccctg
     1321 acgaagcccg attttttcgc ctgctctccg agaacacttc ggtcgtatct cacgatatcc
     1381 cctcaatgag tacgattgat gccggagcat acgcaggagg caatatgatt gccgcgcctc
     1441 aattaaggag gttgcggaac tcggcagggc tcggcccgat atggcaacgt tcggcgggaa
     1501 atggttaggc ttggcgcgtt tcggttcgga ttggcgaggc acggacaaag agagggcggc
     1561 gcaatgtcgc cctctctttt ctcgtgagag catcgtatag aatagaaaag cagcgagcat
     1621 gacgacgagt tcttcactgt cgggcatcag actgagcgac ccgccggtcg ctttcgactc
     1681 ttgaagtgct gtatataaat gagaaaatag taagaaaccc ggtgtacgtc agataaaaca
     1741 gcaatctgaa actgccggac gcgataattt gcgcaacggc actgcaaaaa gaggctgttc
     1801 tgattaccgc tgacaagcag cttataaaat cagcgcagca catcggggtg agggcgaaaa
     1861 acgcattgta gggtaaccgg tgcggatttt cgggaaagga gtgagcgagg atgccggagg
     1921 ttttcgtcac aggcgacaaa catggagaga tcgaggtaaa ggacctctcg cttcggcgtt
     1981 ttcccgcagg aaacgtcctg acgaagcgag acttcgtcgt gatacttggc gacttcggcc
     2041 tgctctgggg gaatccgcct agggactcgg agcgctactg gctgaactgg ctctcggaga
     2101 aaaagtggac tacccttgta atcgacggca accacgagaa cttcgacctg ttcgactcgc
     2161 tgcctcagga agaacggtgg ggcggtcccg tcggcgtcat cgctccggac gtctaccact
     2221 tgaagcgtgg ctatgtctac gagatcgccg gaagaaaggt gttcgtcttc ggaggaggag
     2281 agagcacgga caaggacgga agaatccccg gcaagagctg gtggccccgt gagttgccct
     2341 cctacgccga gaccgagcgc ggcttcgaaa cccttgaacg ggcaggttgg aaggttgact
     2401 gggtatggac gcacgccgct ccccgcagag ccttcgacga gttgatggaa aaccactacg
     2461 ccgtcgcaca cctgcagaga gagaagatgg acgaccccgt atcgcgctat cttgacgagc
     2521 tggccgggag gctggagttc tcgttgtggt gtttcggcca ctaccatctc cagtcacgtc
     2581 ctttcgaggc gggggcgaac ggtcttttcc gcgcggaata cagggaagtc cgccc
//
LOCUS       DAOTIL010000337         2632 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminobacteriaceae bacterium isolate AMR_MDS_3026
            NODE_46267_length_2632_cov_4.206054, whole genome shotgun sequence.
ACCESSION   DAOTIL010000337 DAOTIL010000000
VERSION     DAOTIL010000337.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868050
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminobacteriaceae bacterium (wastewater metagenome)
  ORGANISM  Aminobacteriaceae bacterium
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae.
REFERENCE   1  (bases 1 to 2632)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2632)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:19:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,224
            CDSs (total)                      :: 3,198
            Genes (coding)                    :: 3,184
            CDSs (with protein)               :: 3,184
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2632
                     /organism="Aminobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_46267_length_2632_cov_4.206054"
                     /isolate="AMR_MDS_3026"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:3034609"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(603..962)
                     /locus_tag="P5201_07665"
     CDS             complement(603..962)
                     /locus_tag="P5201_07665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003156585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HRV98449.1"
                     /translation="MEKKRKFNCTIEASMCFIGGKYKPLILWHLIEGTKRFSALQRLL
                     PQATPRMLTMNLRELESDGLVHRKVYPEVPPKTEYSLSDLGKTLIPLITELREWGRYY
                     FEVTGQPNPCDVPSAEK"
     gene            1224..2357
                     /locus_tag="P5201_07670"
     CDS             1224..2357
                     /locus_tag="P5201_07670"
                     /inference="COORDINATES: protein motif:HMM:NF014609.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PhzF family phenazine biosynthesis isomerase"
                     /protein_id="HRV98450.1"
                     /translation="MLKYYVADAFADEVFEGNPAGVCILDEWLSEDLMKKIAMENNLS
                     ETAFAVKEDERTYGLRWFTPVGEIDLCGHATFGTAFVLFRFEEQTADTLHFHAKKCGH
                     HLIVAKNGDMLAMRFPTIAPERYAYAEYMGEGMGAVPSEVYRTERDLLLVYDSEETIR
                     NMKPDFAKLKEFPVGLSVYVTAKSSGNDYDIVARAFWPKINVNEDPVCGTMYCTLMPF
                     WRERLGKDTLVARQLSARGGTVYCSYNGDSVGISGRGALYSVGTIFVNGSNEYSISKI
                     NTTDTAELCEISVENKRALGISVNIPGTELQILQIRCQKGMLFCRVFDTSVVEKLQFP
                     AAIFSAPKFEDMLNNNPIHLSKAAVALGATADMTGRELVKLFS"
BASE COUNT          657 a          697 c          710 g          568 t
ORIGIN      
        1 ggaggcgacg gggagagagg aaaccccgta gagaggttcc tccctcgaaa tgggaacctc
       61 gtggagttca ccgcctcggc gacggtctcg acgtcaaggt cttcgacacg gttcgagcgg
      121 atggaatcaa cagccttcca gaccacggca tgcatatcca tgagcggccc ctccttcctg
      181 aacggaaatg atacgcgccg cccgagagcg cgtcaaaccc atgccttcac cgcccagggt
      241 ttgcaataac gcccgggcac gtcgcatata ctcttgcatt tgttccgaaa cgatactatg
      301 cttgcatggt cagagaacga cgcatattct gcagggtatt ctgaatcaca agtaaaacgg
      361 ctctcacgaa gggaggcccg ggcgaatgaa tacagtcact atttccaacg agtaccagat
      421 agtaatccca aaggagatcg gagacgcgat cggcgcaaag ccggggactc gactggaggt
      481 tctgtcgtac gacgaaaaaa tcgttctcgt gccgattcgc cctatgagga gcatgaaagg
      541 tgcttttaag ggaatcaata cggagatcga gcgggcaaag gattccgcga caccttggaa
      601 atttacttct ctgcgctcgg cacatcgcac gggttcggct gtcccgtaac ctcgaaataa
      661 taccttcccc attcgcgtaa ttccgtgatc aacgggatca gagtctttcc caaatccgag
      721 agagagtatt cggtctttgg aggcacctcc gggtatacct ttcggtgaac gagaccgtcc
      781 gattccaatt cccgcagatt catcgtcagc atacggggcg tcgcttgcgg aagaagccgc
      841 tgcagagcgg aaaaccgctt cgttccctcg atcagatgcc acaggatcag cggcttgtat
      901 ttcccgccga tgaagcacat cgacgcctcg atcgtacagt tgaattttct ctttttttcc
      961 atgaggatac ccccgctata gtttttgata gtacctataa gaaatgtgcg tacttgcgtc
     1021 ttccatatca ttatcctata gtaaaaagac agacgtatca aacacggcga acgagtagcc
     1081 gccgatatct ccactcgcga caagtgtctc cgagtatttc aagacgcttc gcggatgccg
     1141 gtacgaccgg catggcgatc cgctctcatc ggggattctg gagtgaaacg atcacgtgcg
     1201 aatacctgga aggaggaaaa acgatgctga agtattatgt tgcggatgcc ttcgccgacg
     1261 aggtcttcga agggaatccg gcgggcgtgt gtatccttga cgagtggctt tcggaagacc
     1321 tgatgaaaaa aatcgccatg gaaaacaatc tgtcggaaac agcattcgcg gtgaaagaag
     1381 acgagcgtac atacggcctg cgctggttta ccccggtcgg ggagatcgat ctttgcggac
     1441 acgccacctt cggaacagcc ttcgttctgt ttcgcttcga ggaacaaacg gccgacacgt
     1501 tgcatttcca cgcgaaaaaa tgcggtcatc acctgatcgt cgcaaagaac ggcgatatgc
     1561 tcgcgatgcg ctttccaacc atcgctcccg agcgatacgc ttatgccgag tatatgggcg
     1621 aggggatggg cgccgttccc tccgaggtgt acagaacgga gcgcgacttg ctgctggtgt
     1681 acgattccga agagacgatc cgaaatatga agcccgactt tgcgaagctc aaggaattcc
     1741 cggtcggtct ctcggtctat gtgaccgcga agagcagcgg caacgactac gatatcgtag
     1801 cgcgcgcatt ctggccgaaa atcaacgtca acgaagatcc ggtgtgcgga acgatgtact
     1861 gcacgctgat gcccttctgg cgcgaacggc ttgggaaaga cacgctggtg gcgcggcagc
     1921 tttcagcgcg aggcggaacc gtctattgtt cgtacaacgg cgacagcgtc ggcatcagcg
     1981 gaagaggcgc gctctattcc gttggaacca tttttgtgaa cggatcgaac gagtattcca
     2041 tatcgaaaat caatacgacg gatacagccg aactctgcga aatttccgtg gagaacaagc
     2101 gcgcgctggg catttccgta aacattcccg gtaccgaact ccaaattcta cagattcgat
     2161 gccaaaaagg gatgcttttt tgcagagttt tcgatacgtc ggttgtcgaa aaacttcaat
     2221 tcccggcggc aatcttttcc gctcccaagt ttgaagacat gctgaacaac aaccccatcc
     2281 atctcagcaa ggccgccgta gcgttgggcg caacagccga tatgaccgga cgcgagttgg
     2341 tgaagctgtt tagttgagcg gtcgctctac aaaaccggac gggaacaaca cattcgtatt
     2401 tgagcgaaat ggaatttcct gttgaaatga ggcgaaggga cggccgcccg agagcgcgtc
     2461 aaacccatgc cttcaccgcc cagggcttgc aatagcgccc gggcacagcg catatacttt
     2521 tgtatttgtt ccgaaacgaa acgatactct gcttgctcgc tcggagaacg acgcgtattc
     2581 tgcaggatat tctgaatcaa aaagtaaaac ggctttcgcg aagagagtcc tg
//
LOCUS       DAOTIL010000338         2632 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminobacteriaceae bacterium isolate AMR_MDS_3026
            NODE_46272_length_2632_cov_3.309662, whole genome shotgun sequence.
ACCESSION   DAOTIL010000338 DAOTIL010000000
VERSION     DAOTIL010000338.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868050
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminobacteriaceae bacterium (wastewater metagenome)
  ORGANISM  Aminobacteriaceae bacterium
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae.
REFERENCE   1  (bases 1 to 2632)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2632)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:19:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,224
            CDSs (total)                      :: 3,198
            Genes (coding)                    :: 3,184
            CDSs (with protein)               :: 3,184
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2632
                     /organism="Aminobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_46272_length_2632_cov_3.309662"
                     /isolate="AMR_MDS_3026"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:3034609"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1858
                     /locus_tag="P5201_07675"
     CDS             <1..1858
                     /locus_tag="P5201_07675"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRV98451.1"
                     /translation="GAVAPFYPDIAKLKIKGAVTAKAVITGKPAAPKIDLTLSSGSLG
                     LLDSVSFKNLKATTSLVGDPKALEKADIDLNISAASAAAEGIGLSDLALKLKKSGQKI
                     TILSAGAKSGAGSVSAAGAVALPAKAGEKGNLDLTVTIAKADLAFLAAAGGLGVPLAG
                     TLDGSVALKGALDAPSLVLKASSPKISVAGMTATDLSAALSGTMKDMKIDDFKAKFGG
                     GSLSAKGGVTLGATPAVTLDISGSDLDLAALVSGMPDAKELGIGGKLNVSFNGRFAGA
                     AGKGQGSISSPAFTVMGLKGSNLSYPIALDGNSISSKGASLSFYGGTLRGGGALDIKT
                     MKFSHTVELNGVDVNSVAQDFTGGLEGKIGGLAKGSASISGALEPKLAYSGKGSVTIG
                     EGSITGFKAVQLLTALYRSGVRYSSVTAPFRLETGRFILEKGTKANAPQNDPLYKFLT
                     AEGPIGPKGALNLQCAGNVNLQVLNALTGGAVGGLTAGSLEDALKGLLGGLQSGMEKA
                     DFRDISFTLKGTTEKPGVSNLKIAPGAQQPGQPAPTGQPAEPQDPKKKLEQMLIEQIV
                     KPSKPAQPEQPQQMQPAQPDAPASSPTPAPTPTPEKKPEDIIKDKILESIFK"
     gene            complement(1974..>2632)
                     /locus_tag="P5201_07680"
     CDS             complement(1974..>2632)
                     /locus_tag="P5201_07680"
                     /EC_number="3.4.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005660306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016805 - dipeptidase activity [Evidence
                     IEA];
                     GO_function: GO:0070004 - cysteine-type exopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="C69 family dipeptidase"
                     /protein_id="HRV98452.1"
                     /translation="FGYPDRFYGPYDGAGDVGDPKRVLEGAWERPLSVTYCGYVFVAQ
                     ARSWLPSPVGGRMWLGLDKPSDTVFIPFHIGVNRLPSSVEVCDTTKFSRESAWWTFNF
                     LANYAGLKYSYMHKEIADMQDQLENGFLETLEIVERKAVDLHRTNPAGARAYLTEFAE
                     RNTAVTLKEWWNLAEHLIVKYNDGLLTEGGKIGRPLGYPKEWLKTTSWPEGPTSYRKR
                     "
BASE COUNT          545 a          784 c          786 g          517 t
ORIGIN      
        1 tggcgccgtc gcgccgttct atccggacat cgccaagttg aagatcaagg gcgcggtgac
       61 cgcaaaagct gtgatcacag gcaaacccgc cgctccgaag atcgacctga cgctctcctc
      121 tggcagcctc ggccttctcg acagcgtctc cttcaagaat ctgaaggcga cgacatcgct
      181 ggtcggcgac ccaaaagcgc ttgaaaaagc ggatatcgat ctgaacatct ccgcagcatc
      241 ggccgcagcg gaaggcatag gtctttcgga ccttgcgctg aagctgaaga agtccgggca
      301 gaagatcacg atcctctctg caggagccaa gagcggggca ggttccgtct ccgccgccgg
      361 ggcggttgcg ttgccggcaa aagccggaga aaaaggaaat cttgatctca ccgtcaccat
      421 agcgaaggcc gatctggcgt tcctcgccgc cgccggaggg ctgggagttc ctctcgccgg
      481 aacgctcgac ggttcggtcg cgctcaaagg cgctctcgac gcgccgtcgc ttgttctcaa
      541 ggcctcctct ccaaagatct ccgttgcggg aatgactgcg acggaccttt ccgccgcgct
      601 ttcaggcact atgaaggaca tgaagatcga cgattttaag gcaaaattcg gcggagggtc
      661 tctctctgcg aagggcggcg ttacactggg ggcgacgccc gctgttacgc tcgatatttc
      721 gggaagcgat ctcgatcttg ccgcgcttgt ttccgggatg ccggatgcga aggagctggg
      781 catcggcgga aaacttaacg tctccttcaa cggacgtttt gcaggagcgg cgggcaaggg
      841 acaaggctct atctcctctc cggcgtttac tgtcatgggc ctgaagggct cgaacctttc
      901 gtacccgatc gctcttgacg gaaactccat ctcctcgaag ggggcctctc tctccttcta
      961 cggaggtacc ctgcgaggag gcggcgcgct cgacatcaag acgatgaagt tctcgcacac
     1021 cgtcgagctc aatggggtgg atgtgaacag tgttgcgcag gatttcacag gcggtctcga
     1081 aggaaagatc ggcggtctcg ccaaggggtc cgcgagcatc tccggcgcgc tcgagccgaa
     1141 acttgcgtac tccggaaagg ggagcgtgac catcggagag gggagcatca ccggcttcaa
     1201 agcggtgcag ctcctgacgg cgctctatag gtcgggggtg cgctacagca gcgtgaccgc
     1261 ccctttcaga ctggagacag gacgctttat tcttgagaag ggaacaaagg cgaacgcgcc
     1321 gcagaacgat cctctctata agttcctcac ggccgaaggg ccgatcggtc ccaagggggc
     1381 gctcaacctt cagtgcgcgg ggaatgtcaa tcttcaggtg ctcaacgcgc ttaccggagg
     1441 cgccgtcggc gggctgaccg ccggttcgct ggaagatgcg ctgaaagggt tactgggcgg
     1501 cctgcaaagc ggtatggaga aggccgattt cagagatatt tcctttactt tgaaaggaac
     1561 gacggagaaa cccggcgtct ccaatttgaa gatcgccccg ggtgcgcaac agcccgggca
     1621 gcccgctcct acgggacagc cggccgagcc gcaggatccg aaaaagaagc tggaacagat
     1681 gctcatagag cagattgtga agccttcaaa gcctgcgcaa ccggagcagc ctcagcagat
     1741 gcagcctgcg cagcccgacg cgcccgcttc gtctcctacg cctgcgccga cgccgactcc
     1801 ggagaagaaa ccggaagata ttatcaagga caaaattctc gaatccatat tcaaataata
     1861 gcggaaagaa cgaacagggg aggcgtacgc ctcccctgtt ttgtatgacc acaactcttt
     1921 ttcgctcccg atggagattc gtatccctca aagcgggatc agttggaatg tgcttatctc
     1981 ttccggtagc tcgttggtcc ctccggccac gaggttgtct tcagccactc tttcggatag
     2041 cccaaaggcc ggccgatctt tcctccctcc gtcagcagcc cgtcattgta cttcacgatg
     2101 agatgctcgg ccagattcca ccactctttc agcgtaaccg ccgtgttccg ttcggcgaat
     2161 tcggtgaggt aggccctcgc tccggccggg ttcgtcctgt gcaggtcgac tgctttgcgt
     2221 tcgacgatct ccagcgtctc caggaaaccg ttttcaagct ggtcctgcat atcggcgatc
     2281 tccttgtgca tgtagctgta cttgagcccg gcatagtttg cgaggaagtt gaatgtccac
     2341 cacgcgctct cgcgggaaaa tttcgtcgta tcgcagactt cgaccgagct cggaagacgg
     2401 ttgacgccga tgtggaacgg gatgaagacc gtgtcggacg gcttgtcgag accgagccac
     2461 atcctccccc cgacgggcga ggggagccaa ctcctggctt gggcgacgaa cacgtagccg
     2521 cagtacgtca cagaaagcgg acgttcccat gcgccttcca gcacgcgctt cggatccccg
     2581 acgtcgccgg cgccgtcgta cggaccatag aacctgtcgg ggtagccgaa cg
//
LOCUS       DAOTIL010000339         2630 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminobacteriaceae bacterium isolate AMR_MDS_3026
            NODE_46318_length_2630_cov_4.064466, whole genome shotgun sequence.
ACCESSION   DAOTIL010000339 DAOTIL010000000
VERSION     DAOTIL010000339.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868050
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminobacteriaceae bacterium (wastewater metagenome)
  ORGANISM  Aminobacteriaceae bacterium
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae.
REFERENCE   1  (bases 1 to 2630)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2630)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:19:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,224
            CDSs (total)                      :: 3,198
            Genes (coding)                    :: 3,184
            CDSs (with protein)               :: 3,184
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2630
                     /organism="Aminobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_46318_length_2630_cov_4.064466"
                     /isolate="AMR_MDS_3026"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:3034609"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..205
                     /locus_tag="P5201_07685"
     CDS             <1..205
                     /locus_tag="P5201_07685"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRV98453.1"
                     /translation="VTLFWTEEDFKESTNKGFFRVEGDRYSVRVEDGSVVSWRRGEPG
                     VSLEMAKTLGAVLERYPYYMMNL"
     gene            218..1546
                     /gene="ssnA"
                     /locus_tag="P5201_07690"
     CDS             218..1546
                     /gene="ssnA"
                     /locus_tag="P5201_07690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008713423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative aminohydrolase SsnA"
                     /protein_id="HRV98454.1"
                     /translation="MLLIGNGKVITRDENNTVIENGAVVIDGAHIKAVGKTEDLKKAY
                     PGATFKDAKGRLVMPGLINVHMHYYSTFARGIALDSPPATKFSEILEGLWWRLDKCLT
                     LEDVYYSVMGPGIDQIKCGVTSVIDHHASPFAVRGSLAKIAEASAELGLRSNLCYEVS
                     DRDGEQSRVDGITENIEFIKACAAKKDDMLKGMFGLHASMTISDKTLNESVEQMAGLD
                     AGFHVHVAEGIEDLEDSKAKYGLGVVERWHKHGVLGPKSLAIHCIHISEKEMDLLKES
                     DVAVAHNPESNMGNAVGLSPVLPMMKKGVLVGLGTDGYTADMFESYKVANCLHKHGAK
                     LPSVAWTEPPTMLFENNKKIMNRQIDGEVGTLKPGAYADVVIVDYDPPTPLHGGNING
                     HLLFGVCGRHVSTTIANGKVLMDERKLVHVDEEKIMAESRALAKKLWERI"
     gene            1695..>2630
                     /locus_tag="P5201_07695"
     CDS             1695..>2630
                     /locus_tag="P5201_07695"
                     /inference="COORDINATES: protein motif:HMM:NF014758.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase family protein"
                     /protein_id="HRV98455.1"
                     /translation="MTERLNVVGANVPRHDALAKVTGRARYVADLPCDGAWVGGALWS
                     RTPRARVRNIVFSPDFDWSRVTVITARDLPGPNEVHMIKDDYPILAGDEISYIGQAIA
                     LVAAPDKQTIEEALGAMRPDLEELPPVLSVEASLAKEAVIWGEDNVQAHYRIEHGDLE
                     EAFSRSDVIIEETYRTHHQEHLYLETNGMFARPRPDGGVIVEGSLQCPYYVARALAHA
                     LGLPLEKVTMRQTETGGAFGGKEDYPSVLAIQTALLSMKSGKPVRMVLERREDIDVTT
                     KRHPSVIRHRTGVTRDGVILAAEIDIIFDGGAFSTM"
BASE COUNT          602 a          725 c          810 g          493 t
ORIGIN      
        1 ggtcacgctc ttctggaccg aggaggattt caaggagagt acaaacaagg gattcttccg
       61 cgtcgaaggc gaccggtact ctgttcgtgt cgaagacggt tcggtcgtct cctggcgccg
      121 cggcgaaccg ggcgtctccc ttgaaatggc gaagacgctc ggagcagtgc tcgagcgcta
      181 cccctactac atgatgaatc tctaggaggt gcacggcatg cttttgatcg gcaacggaaa
      241 agtcatcaca agagacgaga acaacacagt catagagaac ggcgcggtcg tgatcgacgg
      301 cgcgcatata aaggcagtgg ggaagaccga ggatctgaag aaggcctacc ccggcgcgac
      361 gttcaaggac gcgaaagggc ggcttgtgat gcccggcctt atcaacgtcc acatgcacta
      421 ttacagcacg ttcgcgagag gcatcgcgct cgacagtcct cctgcgacga aattctccga
      481 aattcttgaa gggctctggt ggaggctcga caagtgcctt acgctcgaag acgtctacta
      541 cagcgtcatg gggccgggaa tcgaccagat caagtgcggc gtcacgtcgg tcattgatca
      601 tcacgcgagc ccgttcgcgg ttcgcggcag tcttgcgaag atcgccgaag cttcggcgga
      661 gctgggcctt cggagtaatc tctgctacga agtctccgac cgcgacggag agcagagcag
      721 ggtggacggc attaccgaga atatcgaatt catcaaggcg tgcgcggcga agaaggacga
      781 catgctcaag ggcatgttcg gactgcacgc gtccatgacc atctcggaca agacgctgaa
      841 tgaaagcgtg gaacagatgg cggggcttga cgcgggattc cacgtgcacg tcgccgaagg
      901 cattgaagac ctcgaggaca gcaaggcgaa gtatgggctg ggcgtggtcg agcgctggca
      961 caagcacggc gttcttggtc ccaagagtct cgcaatccac tgcatccata tctccgaaaa
     1021 ggagatggat ctgctgaagg aatcggatgt cgctgtcgcg cacaacccgg agtcgaatat
     1081 ggggaacgcg gtcggcctct ctccggtgct gccgatgatg aaaaaaggcg tcctcgtggg
     1141 tcttggtacc gacgggtaca cagccgatat gttcgaatcg tacaaagtgg cgaactgctt
     1201 gcacaaacat ggggcgaagc tcccgagcgt cgcgtggacg gagccgccga cgatgctctt
     1261 cgagaacaac aaaaagatca tgaaccggca gatcgacggc gaggtcggaa cgctgaagcc
     1321 cggcgcatac gccgacgtcg tcatcgtgga ttacgatcct ccgacgccgc ttcacggagg
     1381 caacatcaac ggacaccttc tcttcggcgt ctgcggcagg catgtcagca ccacaatagc
     1441 caacgggaaa gtcctgatgg acgagcggaa acttgtccac gtggacgagg aaaaaataat
     1501 ggccgagagc cgcgctctgg ccaaaaaact ctgggagcgg atttaacgaa aagacgatcg
     1561 ttgccggagg ggtgagggag cgccctttga agcgactatg cgtcgcgccg gagggcgctt
     1621 cctcacgggc tgtacctgtc ggcggcgttc agtcttctcg tagataatcc catttttaag
     1681 gaggatgcat gcccatgacc gaaaggctga acgtggtcgg cgcaaatgtg ccgcgacatg
     1741 acgcccttgc gaaagtaaca ggccgcgccc gctacgtcgc cgatctgccc tgcgatggcg
     1801 catgggttgg gggagcgctg tggagcagaa ctccgcgtgc ccgggtgcga aatatcgtat
     1861 tttcccccga ttttgactgg tcacgcgtga cggtgattac cgcgcgcgat ttacccggcc
     1921 ccaacgaagt gcacatgatc aaggacgact atccgatcct cgccggcgac gaaatctcct
     1981 atattggtca ggcgatagct ttggttgccg cgcccgataa acaaaccatt gaagaggcgc
     2041 tcggcgcgat gcggccggat ctggaggaac tccccccggt gctcagtgtg gaggcttctc
     2101 ttgcaaaaga agccgttatc tggggcgagg acaatgtcca ggcgcactac cggatagagc
     2161 acggagacct cgaagaggcg ttttcccgtt cggatgtgat tatcgaagag acctacagaa
     2221 cgcatcatca ggagcatctg taccttgaga caaacggtat gtttgcccgc cctcgaccgg
     2281 atggcggggt catcgtcgaa ggttctcttc agtgcccgta ctacgttgcc agagcgcttg
     2341 cgcatgcctt ggggcttcct ctggaaaagg tgacgatgcg ccagaccgag accggcggcg
     2401 ctttcggcgg taaggaggac tatccctcgg tattggccat tcagactgcg ctgctttcca
     2461 tgaaaagcgg aaaacccgta cgaatggtgc ttgaacgcag agaggatatc gacgtcacca
     2521 caaagcgcca cccgtccgtc atccgccatc gcacgggagt cacgcgcgac ggcgttatcc
     2581 tcgcagctga aatagacatc atctttgacg gcggcgcgtt ctccaccatg
//
LOCUS       DAOTIL010000340         2630 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminobacteriaceae bacterium isolate AMR_MDS_3026
            NODE_46321_length_2630_cov_3.979417, whole genome shotgun sequence.
ACCESSION   DAOTIL010000340 DAOTIL010000000
VERSION     DAOTIL010000340.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868050
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminobacteriaceae bacterium (wastewater metagenome)
  ORGANISM  Aminobacteriaceae bacterium
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae.
REFERENCE   1  (bases 1 to 2630)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2630)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:19:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,224
            CDSs (total)                      :: 3,198
            Genes (coding)                    :: 3,184
            CDSs (with protein)               :: 3,184
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2630
                     /organism="Aminobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_46321_length_2630_cov_3.979417"
                     /isolate="AMR_MDS_3026"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:3034609"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(316..1461)
                     /gene="recA"
                     /locus_tag="P5201_07700"
     CDS             complement(316..1461)
                     /gene="recA"
                     /locus_tag="P5201_07700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008710861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008094 - ATP-dependent activity, acting
                     on DNA [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0009432 - SOS response [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase RecA"
                     /protein_id="HRV98456.1"
                     /translation="MAKKKTPQTRDDVLELAIEDIRSKFGEGSIMRLGDSFKAAVEVI
                     STGILPLDVALGIGGLPRGRIVEIFGPEGSGKTTVALHAVAEAQKAGGVAAFIDAEHA
                     LDPRLAHNLGVDVQSLYIAQPDSGEQALYVLDTLVRSSAVDIVVIDSVAALTPQAEID
                     GKIGDSQVGLQARLMSYALRRLTSAISKSNCVVVFINQLRAKISTGYSQGPQETTTGG
                     RALKFYSSVRIEVKRGKGVSKGDDTIGHELWMKVVKNKQAPPFKTGHCTLLYGKGLPK
                     GMSILDMAIDFNVVKRKGSWLAYKGETLGQGKDTVAQYIEEHPDLMQEISAEVLAVTS
                     EGLSLALSEHESPLPLEDDENSVPSLSIEDGILELDEEVETGDEEEK"
     gene            complement(1521..2099)
                     /gene="thpR"
                     /locus_tag="P5201_07705"
     CDS             complement(1521..2099)
                     /gene="thpR"
                     /locus_tag="P5201_07705"
                     /inference="COORDINATES: protein motif:HMM:TIGR02258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008664 - 2'-5'-RNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA 2',3'-cyclic phosphodiesterase"
                     /protein_id="HRV98457.1"
                     /translation="MNGILRSFFCIELPPAVQKKLWQTVDTLRLVLPDLKWVSANALH
                     ITLKFLGEQEQRLLEDLMRSFEKRIASSGQDVIALSSSALGAFPNIRTARTLFAELCG
                     DLVPLKAIASIVEDVSAEHGIPREKRPFKPHITLARTRRTETVIISPKDIPLFSKIEW
                     QATSVTLMKSSLTPAGPIYTPLKIWEFPAKVF"
     gene            complement(2096..>2630)
                     /locus_tag="P5201_07710"
     CDS             complement(2096..>2630)
                     /locus_tag="P5201_07710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009202380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="CinA family protein"
                     /protein_id="HRV98458.1"
                     /translation="ARTLRELLPGDCLPEGCFSVAEDLLREARKRNVTLAFAESCTGG
                     LLGAALTDISGSSDVFLGSAVCYSNSAKKHILSVSDELLESFGAVSSECAIAMAKGAL
                     NQFGSELALSVTGVAGPGGGSEEKPVGAVWFALAGLCGERAFLHHFPGDRLQIRRSAM
                     NTGLLLLRKTVMELNIL"
BASE COUNT          677 a          674 c          598 g          681 t
ORIGIN      
        1 cgttcgtcct gagccaggat caaactctcg aatatatttc tcggaacgcc tcttacctct
       61 tcttctcgct cttataaaac tgtcaaggtt ctgctcgggt cacacccgaa acacttggcc
      121 tcaaccaaag tgctagagta tgatacctga cgtaccagat gttgtcaacg caaacatcct
      181 gtaattgcaa tgaagagtca tgcaatctgc tagagataat aagcgaagaa taagaatcgc
      241 tattagcatt atttaaagac agtatctaac tataaaagca caagctggtt atttataaag
      301 cttttcccct tattgttatt tttcttcttc gtctccggtt tctacctctt cgtcgagttc
      361 aagaattccg tcttcgattg aaagagaagg aacggaattt tcatcatcct caagaggaag
      421 aggagactca tgttccgaaa gtgcaagcga gagtccttct gaggtaacag caagtacttc
      481 tgcggaaatc tcctgcatca ggtcaggatg ttcttcaata tactgcgcaa cggtatcttt
      541 cccctggcct aatgtttctc ctttataagc aagccatgag ccttttcttt ttacaacatt
      601 aaagtcgatg gccatgtcga gaatcgacat gcccttagga agtcccttcc catagagcag
      661 agtacagtgg ccggttttga agggtggcgc ttgcttgttt ttgacaacct tcatccatag
      721 ttcatgcccg attgtgtcgt ctcctttact aacgcctttc ccccgtttta cttctatccg
      781 cacagagcta taaaatttga gagcgcgccc tccggttgtc gtttcttgag gcccctgcga
      841 atagcctgtg ctgatttttg cgcgcaactg gtttataaaa accacgacgc agttgctttt
      901 cgaaattgct gaggtcagtc gtctgagagc ataggacata agacgagcct gaaggcctac
      961 ctggctatct ccaatcttac cgtctatttc cgcctgcggg gtcagagcag cgactgaatc
     1021 gataaccaca atatcaacgg cgctgctgcg aaccagcgtg tccaaaacgt aaagagcctg
     1081 ttctccgctg tcgggctgtg cgatatataa ggactgcacg tcaacgccga gattatgcgc
     1141 aagtctcggg tccagagcat gttcagcgtc aataaaggcg gcaactcctc cggctttctg
     1201 cgcttcagca acggcatgca gagctacggt tgttttcccg gagccttcag gcccgaaaat
     1261 ttcaacgata cgaccacggg gaaggccacc gatacccaga gccacatcca aagggagaat
     1321 acctgttgaa ataacctcaa cagccgcttt aaaggaatct ccgagacgca ttatggagcc
     1381 ttctccaaat ttgctgcgga tatcctcaat ggcaagttca agaacgtcgt cacgcgtctg
     1441 aggagttttc ttcttcgcca aacaaattcc tccttccaga atatgaacag tagtattgta
     1501 caggaaaagt cgtgttccta tcaaaaaact ttcgccggga attcccagat ttttagaggg
     1561 gtatagattg ggccggcagg tgtcagagaa cttttcatca acgttaccga tgtcgcttgc
     1621 cactctattt tactgaaaag agggatatct tttggggata ttatcactgt ttccgtacgg
     1681 cgcgttctcg caagtgttat gtgaggcttg aaaggtcttt tttctcgtgg aattccatgt
     1741 tccgcactca catcttcaac aattgacgca atagctttca gcgggacaag atcgccgcac
     1801 agttcggcaa aaagagttcg ggccgttcgt atatttggga aagcaccgag agcggaagag
     1861 gaaagagcta tcacatcttg cccggaagaa gcaattcttt tctcaaatga tcgcataaga
     1921 tcctcgagca gccgttgctc ctgttcgccg agaaacttga gcgttatgtg gagggcgttc
     1981 gccgataccc atttcaaatc cggcaaaact aaacgaaggg tgtccacagt ttgccaaagt
     2041 ttcttttgta ctgcaggagg caactcgatg caaaagaaag aacgcagtat tccattcata
     2101 gaatgttcaa ctccattacc gttttccgga gcagcaaaag ccctgtattc atcgcacttc
     2161 tacgtatctg gagtctgtcc ccggggaaat gatgcaaaaa tgcacgttcg ccgcaaagac
     2221 ctgcaagagc gaaccagacg gcgcctaccg gtttttcttc agagccgcct cctggtcccg
     2281 cgactcctgt gacagagagc gccaactcgg agccaaactg attcaaagca cccttcgcca
     2341 tggcgatcgc gcactcggaa cttacagccc caaaactctc aagcagctcg tctgaaactg
     2401 agagtatgtg tttttttgcg ctgttactgt aacagactgc gcttcctaaa aacacatccg
     2461 aactccctga gatgtctgtg agcgcagccc caagaagacc tccagtgcat gattccgcaa
     2521 aggcaagtgt gacgtttctt ttccgggcct ctcgaaggag atcctctgct acggaaaaac
     2581 agccttccgg caggcagtcg ccagggagga gctctcgtaa cgtgcgagcc
//
LOCUS       DAOTIL010000341         2630 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminobacteriaceae bacterium isolate AMR_MDS_3026
            NODE_46327_length_2630_cov_3.255534, whole genome shotgun sequence.
ACCESSION   DAOTIL010000341 DAOTIL010000000
VERSION     DAOTIL010000341.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868050
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminobacteriaceae bacterium (wastewater metagenome)
  ORGANISM  Aminobacteriaceae bacterium
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae.
REFERENCE   1  (bases 1 to 2630)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2630)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:19:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,224
            CDSs (total)                      :: 3,198
            Genes (coding)                    :: 3,184
            CDSs (with protein)               :: 3,184
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2630
                     /organism="Aminobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_46327_length_2630_cov_3.255534"
                     /isolate="AMR_MDS_3026"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:3034609"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1167
                     /locus_tag="P5201_07715"
     CDS             <1..1167
                     /locus_tag="P5201_07715"
                     /inference="COORDINATES: protein motif:HMM:NF012422.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LuxR C-terminal-related transcriptional
                     regulator"
                     /protein_id="HRV98459.1"
                     /translation="WRGASLPAPLLLFALVLPSFGSAVHLASWQWRLSQAPFSRQAVS
                     FGLACLVRSSAILLLSFAPRPLLHTVALLLPFLGSFLWNPPGAVLPPDASRGGFRLSP
                     RLAFRVASFFIVSAMFLSILLAQGSGEALAPKVLTDPFYTLGALGAGLALLRWFAVDL
                     RSIYFFAQSLLVLGFMTFAALGGGRPLIPLALLQLGFGTFGAYLFTLLLYLGSRAGRS
                     EALSVVSSIQAVNSGSVVLGMLLTRTTGFAAGLLEVPFVLAASLLGVGLLFFSALFLR
                     DDPTSFAGFDLRGASEEEVPSKEDGAEPPGEEAEPGEREDGVFLRFMEQGLSLQEIRV
                     ALLVDEGLSNADISQRLNITSNTLRSHLRKIHKKIGSSSRKALREELQRMRGDA"
     gene            1426..1869
                     /locus_tag="P5201_07720"
     CDS             1426..1869
                     /locus_tag="P5201_07720"
                     /inference="COORDINATES: protein motif:HMM:NF015444.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HRV98460.1"
                     /translation="MNDTKIGVSGGRFLAFRLAPGEELLGALRRIVAANSIRAGYVAG
                     VVGSLSRACLRFAGRHDGAALDGCFEVVSLVGTLDAEGEHLHMTISNQEGTTTGGHVL
                     EGCVVRTTMELVLGDLSDLEFRREYCPLSTYGELAVIPRKNNKGE"
     gene            1873..2436
                     /locus_tag="P5201_07725"
     CDS             1873..2436
                     /locus_tag="P5201_07725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005660476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HRV98461.1"
                     /translation="MKNRTLVLVALCIALNMGLGQIASTLKLPIFMDSIGTVLSAILM
                     GPWIGMLTGLCTNLIWGVLTSPVAAAFAPVAMVIGLVAGVLARKGMFRSLPLVIVSGI
                     VVTIFVTLVATPIRTYLFAGVTGSGADFFVAYLTAVGQKLLQSVAITVIWTNLVDKIV
                     ACFVAWLLVKQLPERVKSLFPGASDVA"
BASE COUNT          387 a          776 c          853 g          614 t
ORIGIN      
        1 tggcggggag cttcgcttcc ggctcctctg ctcttattcg cgcttgttct tccgtccttc
       61 gggtcggcgg tgcatctggc gagctggcag tggaggctct cgcaggcgcc gttttcgcgc
      121 caggcagtct cgttcggact ggcatgtctg gtccgaagct ccgctattct cttactctca
      181 ttcgctcccc gaccgctgct ccatactgtc gcgctgctcc ttccctttct ggggagcttt
      241 ctatggaacc ccccaggagc ggttcttccg cccgacgctt ctcgcggcgg gttccgtctc
      301 tcgccccggc tggcgttccg tgtggcttcc ttcttcatcg tctcggcgat gtttttgtcg
      361 attcttctgg cgcagggaag cggtgaagcg ctcgcgccaa aggtgctgac ggatcctttc
      421 tacacactgg gggcgctcgg agcgggactt gcgcttctcc gctggtttgc cgtcgacctg
      481 aggagtatct acttttttgc gcagtccctg ctggtgctgg ggttcatgac gttcgcggcg
      541 ctcggcggcg ggcgtccgct gattccgctc gccctgttgc agctcggctt cggaacgttc
      601 ggcgcctacc tctttacgct gctgctctat cttgggtcgc gcgccggaag atcggaggcg
      661 ctttcggtgg tgagctcgat acaggctgta aacagcggct ctgttgttct gggaatgttg
      721 ctgacccgga cgacaggctt cgcggccggt ctgctcgaag tgccgtttgt tcttgctgcc
      781 agtcttctgg gcgtagggct gctttttttc tcggcgctct ttctccggga cgatccgacg
      841 agcttcgcgg gcttcgatct ccgcggcgct tcggaggaag aggtcccgtc gaaggaggac
      901 ggagcggagc ctccgggaga ggaagcggag cccggggagc gcgaagacgg ggtgtttctg
      961 cgttttatgg aacaggggct ctcgcttcag gagattcggg tggcgctgct tgtcgacgaa
     1021 ggactttcga acgcagatat ctcgcaacgc ctcaacatca catccaacac gctgcggagc
     1081 catctgcgga agatccacaa gaagatcggt tcatccagca gaaaagccct ccgtgaggaa
     1141 cttcaaagaa tgcgcggaga tgcgtaaagc ggtgcggatg ttgtgcggac gtatggtctc
     1201 tgaaatcgtg atgacgggcg atccgcccgc aatcattcaa caatatgtcc ttcggactcc
     1261 cggtatgtat cctcgcccgg agcaggctgt ttgacacgga gtctttcccc tttatccctc
     1321 cccgcacctt gtcgcaatat ctttttatgt tataattctc atcgctattt gtatggcgta
     1381 ttccgcgatg accgcggaga ataacggtat tccagggagg acgcaatgaa cgacacgaag
     1441 atcggcgtct ccggcgggcg gtttttggcc ttccggctgg ctccggggga agagcttctc
     1501 ggggcgctgc ggcggatcgt cgccgcgaac agtattcggg cggggtatgt cgccggagtg
     1561 gtcggcagtt tgtcgcgggc gtgcctgcgc ttcgcgggac gtcacgacgg ggcggcgctc
     1621 gacggctgct tcgaggtggt ttcgctcgtc gggacgctgg acgcggaggg cgaacacctg
     1681 cacatgacga tctcgaacca ggagggaacg acgacggggg ggcacgtgct ggaagggtgc
     1741 gtcgtccgga cgacgatgga gctcgtgctg ggcgacctct ccgacctgga gtttcgccgc
     1801 gaatactgcc ctctttccac atacggggaa cttgcggtca taccgcgaaa aaacaataaa
     1861 ggggaatgaa cgatgaagaa cagaacgctg gtacttgtag ctctttgcat cgcgctgaac
     1921 atggggttgg ggcagatcgc ctccacgctg aaactgccga tctttatgga ttcgatcggg
     1981 acggtgctct ccgccatcct gatggggccg tggatcggga tgctcaccgg gctgtgcacc
     2041 aacctgatct ggggcgtgct gacgagtccc gtagcggcgg ccttcgcccc cgtggcgatg
     2101 gtcattggcc tcgtcgcggg cgtgctggcg cgcaagggga tgttccgctc gctgccgctc
     2161 gtgatcgtct ccggcatcgt agtgacaatc ttcgtaaccc tcgtggccac gccgatccgc
     2221 acctacctct tcgccggagt caccggttcc ggcgccgact tcttcgtcgc ctacctgacc
     2281 gccgtcgggc agaagctgtt gcagtcggtg gcgatcacgg tcatctggac gaatcttgtg
     2341 gacaagatcg tggcgtgctt cgtcgcctgg ctgctcgtga aacagcttcc ggagcgcgtc
     2401 aagtcgctct tcccgggcgc ttcggacgtc gcctgaggcg ccgtggggcg actgcgcgca
     2461 ctttcggggc ctttgggctc cttcgttttg tggggactct gggtcggggg ctcgatgtcg
     2521 ctcccggccc ttcccgttgt cgccgtcatg gcgggccttt ccgcagtcgc gctctgcgtc
     2581 tggccaaagt cgcgaagcag tgtgaaaggg gtcgcgctct tcatggcgcc
//
LOCUS       DAOTIL010000342         2627 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminobacteriaceae bacterium isolate AMR_MDS_3026
            NODE_46418_length_2627_cov_3.865474, whole genome shotgun sequence.
ACCESSION   DAOTIL010000342 DAOTIL010000000
VERSION     DAOTIL010000342.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868050
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminobacteriaceae bacterium (wastewater metagenome)
  ORGANISM  Aminobacteriaceae bacterium
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae.
REFERENCE   1  (bases 1 to 2627)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2627)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:19:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,224
            CDSs (total)                      :: 3,198
            Genes (coding)                    :: 3,184
            CDSs (with protein)               :: 3,184
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2627
                     /organism="Aminobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_46418_length_2627_cov_3.865474"
                     /isolate="AMR_MDS_3026"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:3034609"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..740)
                     /locus_tag="P5201_07730"
     CDS             complement(<1..740)
                     /locus_tag="P5201_07730"
                     /inference="COORDINATES: protein motif:HMM:NF028500.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase RecG"
                     /protein_id="HRV98462.1"
                     /translation="MTIRRADLSDPVRFVKGVGPSRERQLANLGVFTAEDLLFLFPRR
                     YEDRRTLTPLSSLRPGTATTVIARVVAVEKRTTAKKQLSLTTVLISDGQGMAKAVWFN
                     RVGLENTFRSGLRVALFGKVELRNGVPQLVAPEFEVLEEDDGEEELLSIVPLYPATAG
                     LHQKTLRRIVRTALEEYLPLLEEFLPVSLRERRRLCELTEAMREIHYPTSRENWKTAR
                     RRLVFDEFFLLQAGLALRRNRAEKPLERA"
     gene            complement(737..1240)
                     /locus_tag="P5201_07735"
     CDS             complement(737..1240)
                     /locus_tag="P5201_07735"
                     /inference="COORDINATES: protein motif:HMM:NF016959.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DivIVA domain-containing protein"
                     /protein_id="HRV98463.1"
                     /translation="MPDLLTARDVESKRFSKAMRGYSATEVDQFLDQVAEDIQKYCVQ
                     YADLERYAARLEDKIREFESLKETLSGTLLMAQKTADAKEEAANRQSEAIILEAKSKA
                     EQIIQEAEASKERERKEINHIRQLKQEFKADFCALLSRFSSLIERDEEDPDENDGAEP
                     TEDDVLK"
     gene            complement(1233..1613)
                     /locus_tag="P5201_07740"
     CDS             complement(1233..1613)
                     /locus_tag="P5201_07740"
                     /inference="COORDINATES: protein motif:HMM:NF016363.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein SepF"
                     /protein_id="HRV98464.1"
                     /translation="MLERMMAFLGLVDPAAEEEEGAGETAELETREARVLKPVPESAV
                     ILIARGLEGVQRKEELALALRKGKLLLVDLRGLEREEGQSLLDFLCGVAFANRGTVLR
                     AASGIFLAAPRKSMIEEWEEEDGA"
     gene            complement(1638..2330)
                     /locus_tag="P5201_07745"
     CDS             complement(1638..2330)
                     /locus_tag="P5201_07745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013048790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YggS family pyridoxal phosphate-dependent
                     enzyme"
                     /protein_id="HRV98465.1"
                     /translation="MVSAIHNNLQRVREKLADAVRKSGRNDEDVRLVAVTKTYPVEDI
                     LEAVRCGVVSIGENRVQELSEKRSLWSGPSIEWRFIGHLQGNKVRKVLEFASTVDSLD
                     SLSLARALSRVAEEKKMRLPVLIEVNTSGEESKHGTAPSDAAPLLESVMKECPALQVE
                     GLMTVGPLTDDEAEVRRAFALLRGIRDSLSGAFGLRLKELSMGMSGDFEWAVDEGSTM
                     VRIGSAIFGKRT"
     gene            complement(2324..>2627)
                     /locus_tag="P5201_07750"
     CDS             complement(2324..>2627)
                     /locus_tag="P5201_07750"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HRV98466.1"
                     /translation="TKMKLYLTLPFFSLENVKSREIALFLETGEHVGVAVPESSVRYR
                     EGKLGVFQVEGNIVKFKEIEGLPVEGHRFFVQKGLQSGNIVVLNAERAKEGKIRLW"
BASE COUNT          574 a          823 c          619 g          611 t
ORIGIN      
        1 gctcgttcaa ggggtttttc ggccctgttc cttcgcagag caagcccagc ctgcaggaga
       61 aagaattcgt cgaagacaag gcgtctcctg gcagtcttcc agttctcccg cgacgtcggg
      121 tagtgtattt ccctcatcgc ctcagtcaat tcgcataagc ggcggcgctc ccgcaaagat
      181 acgggaagaa actcttccag caggggaaga tactcctcca gagctgtccg cacaatgcgg
      241 cgcagcgtct tctgatgcag ccccgcagtc gccggataca gaggaacaat cgagagcagc
      301 tcctcttctc cgtcatcctc ttcaagcact tcaaattcag gcgctacaag ctgaggaacg
      361 ccgttgcgca gctccacctt accgaacaga gcaaccctga gtcctgaacg aaaggtgttc
      421 tcaagtccaa ctctgttgaa ccagacagcc ttcgccatcc cctggccatc ggaaatcagc
      481 acagtggtca gagagagttg cttttttgcc gttgtccgtt tttcaacagc gacaactctg
      541 gcgatcaccg tcgtggctgt tccgggcctg agagacgaaa gcggcgtcag cgttctcctg
      601 tcttcgtacc ggcggggaaa aagaaacagg agatcctctg ccgtgaaaac accgaggttg
      661 gccaactgac gttctctgga agggccgact cccttcacga aacgaacggg gtcggacagg
      721 tccgctcggc gaatagtcac ttgagcacat cgtcctccgt cggttcagca ccgtcgttct
      781 cgtcgggatc ttcctcatcg cgttcaataa gggaagaaaa acgggagagg agtgcgcaaa
      841 agtcggcttt aaactcctgt ttgagctgcc tgatatgatt gatctccttg cgctcccgct
      901 ccttcgaggc ttccgcctcc tgaatgatct gttccgcctt cgacttcgcc tccagtatga
      961 tagcctcgga ctggcggttt gcagcttctt ccttggcatc cgcagttttc tgcgccatga
     1021 gcagagttcc ggagagcgtt tctttcaggc tttcaaactc ccggatcttg tcctccaggc
     1081 gggcggcata gcgttcaaga tcggcatact ggacgcagta cttctggata tcctcggcaa
     1141 cctggtcgag gaattggtca acttccgttg cggaataccc tctcatcgcc tttgaaaagc
     1201 gtttgctttc cacatcgcga gctgtcagca gatcaggcac cgtcttcttc ctcccattct
     1261 tctatcatgc tcttccgcgg cgccgcgaga aaaatcccgc ttgctgcccg cagaacagta
     1321 cctctgtttg caaaagccac tccgcagagg aaatcgagca gactctgtcc ctcttcccgt
     1381 tcaagacccc gaagatcgac gagcagaagt ttccccttac ggagagccag ggcaagctct
     1441 tcttttcttt gtactccctc aagaccgcgg gcgataagaa tcaccgcgct ttcaggaacc
     1501 ggcttcagta cgcgggcctc tcttgtttcg agttccgccg tctcccccgc gccttcctct
     1561 tcttcggcag cagggtcaac gagccccaga aaggccatca ttcgttccaa caaggcatca
     1621 cctcccgctg cggcgtatca ggtacgtttt ccgaagatag cggagccgat acggaccatc
     1681 gtacttccct catcaacagc ccattcaaaa tcaccgctca tccccatgga aagctctttc
     1741 aagcggagac cgaacgctcc gctcaaagaa tccctgatcc cccgcagcag cgcgaaggct
     1801 cttcgtacct ccgcctcgtc atcggtcaaa ggtcccaccg tcatcaggcc ttctacctga
     1861 agcgcgggac attccttcat cacgctctca agaagcggcg ccgcgtcgga aggagcagta
     1921 ccatgcttgc tctcctctcc ggaagtattc acctcaatga gcaccggaag acgcattttt
     1981 ttctcttccg caacacgcga gagagccctg gcgagcgaca atgaatccag ggaatcgacc
     2041 gtggatgcaa attcaagaac cttcctcact ttgtttcctt gcagatgccc gataaaacgc
     2101 cactctatgg aaggaccgct ccaaagactt cgcttctccg agagttcctg tacccggttc
     2161 tcccctatcg agacgacgcc gcaccggacg gcttcaagaa tatcctccac cggataggtt
     2221 tttgtcaccg cgacaagccg aacgtcttcg tcattccttc cgcttttgcg gacagcatcg
     2281 gcaagttttt cccgcacacg ttgaaggtta ttgtgtattg cactcaccat agtcggattt
     2341 tcccttcctt cgctctttct gcattgagga cgacgatatt gcccgactgc aacccctttt
     2401 ggacaaaaaa acgatgtcct tccacgggca gaccttcgat ctccttgaat tttacgatat
     2461 tcccctccac ctggaacacc ccgagctttc cctcgcgata tcgaacagag gattccggca
     2521 cggcaacacc gacatgctcc cccgtctcga gaaacaacgc aatctccctg gatttgacat
     2581 tctcgagaga aaaaaacggc aatgtaagat agagcttcat tttcgta
//
LOCUS       DAOTIL010000343         2625 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminobacteriaceae bacterium isolate AMR_MDS_3026
            NODE_46474_length_2625_cov_2.399611, whole genome shotgun sequence.
ACCESSION   DAOTIL010000343 DAOTIL010000000
VERSION     DAOTIL010000343.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868050
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminobacteriaceae bacterium (wastewater metagenome)
  ORGANISM  Aminobacteriaceae bacterium
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae.
REFERENCE   1  (bases 1 to 2625)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2625)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:19:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,224
            CDSs (total)                      :: 3,198
            Genes (coding)                    :: 3,184
            CDSs (with protein)               :: 3,184
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2625
                     /organism="Aminobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_46474_length_2625_cov_2.399611"
                     /isolate="AMR_MDS_3026"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:3034609"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..280
                     /locus_tag="P5201_07755"
     CDS             <1..280
                     /locus_tag="P5201_07755"
                     /inference="COORDINATES: protein motif:HMM:TIGR02452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="TIGR02452 family protein"
                     /protein_id="HRV98467.1"
                     /translation="VGQPESGDLLRRRITRVLQIAFVFGHSTLVLGAWGCGAFGNDPQ
                     RTAKDFREALETEFAGAFSQIVFAIADWSSERRFLGPFRDVFAAKDLS"
     gene            294..626
                     /locus_tag="P5201_07760"
     CDS             294..626
                     /locus_tag="P5201_07760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014162521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HRV98468.1"
                     /translation="MNVRKGNLAGLPANLPDEEIFEELLRTPSFRMERIVSSGHASPP
                     GFWYDQEEDEWVALLQGEAALEFEDGSADKLRSGDWVFLPAHMRHRVASTSLEPPCIW
                     LAVFARPG"
     gene            841..2226
                     /locus_tag="P5201_07765"
     CDS             841..2226
                     /locus_tag="P5201_07765"
                     /inference="COORDINATES: protein motif:HMM:NF024206.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ankyrin repeat domain-containing protein"
                     /protein_id="HRV98469.1"
                     /translation="MIRRTLLFVHLFFFIYGASFAAALPIDEFIEVCGAGSLEEVHEA
                     VASGADVNATDADGWTALMAAADNGNTEVVAFLVDRGADIHRKDRCGTTALMAAAGGS
                     DTRSVQLLVNAGGNVNERNSGGWTPLFFAARFSEEPDVIALLIASGAEVDVAEADGET
                     PLMIAAWYGENPRIVAALLKGGADVHRKDTKGWTPLSYAACTNKNPEVIAALLRGGAD
                     VDERDEDDGTTPLMNAAACNRNPAVLSTLLRAGADVNAKDTKGCTPLHFAARYAENPE
                     VARILLDAGADVAARDAQWGRTPLLWAAKTSKEPKIVEALLDAGADIHARDNYGRTAL
                     MCAAEECCSHPDVVRALLRGGADVNALTSDGRSALFFAAAFQHNDTIVHLLLKEGADV
                     NIRDTAGVTSLLFAAEYGTRPEILLALIHNGADASVCDADGKRAVDYAEGNSYLKDTE
                     VVQVLKKVSPQ"
     gene            2286..>2625
                     /locus_tag="P5201_07770"
     CDS             2286..>2625
                     /locus_tag="P5201_07770"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRV98470.1"
                     /translation="MRIMMRRVAAILFLCVASLWCSCSAHANAVVLLDTPEAAMDLTE
                     RIMEKVADDNIESGIFLMRPFFRLPKEEFNILLEQSKLQIPVIKDRFGAPLEHELIEQ
                     RFIGKSLMRIV"
BASE COUNT          568 a          632 c          811 g          614 t
ORIGIN      
        1 tgtcggacag ccggaatcgg gggatttgtt acgccgtcgg attacgcgtg tgctccaaat
       61 cgcctttgtc ttcgggcatt cgacgctcgt tttgggcgcg tgggggtgcg gcgcgtttgg
      121 caacgacccg cagcggacag cgaaggactt ccgtgaagca ttggagaccg agttcgccgg
      181 agcattctcg cagatcgtct tcgccatcgc cgactggtct tcggaaaggc gctttctcgg
      241 tcccttccgg gatgtcttcg cggcgaagga cttgtcatag agaggagaaa acgatgaacg
      301 tccgaaaagg caacctggcg gggcttcctg cgaatctgcc tgacgaagag attttcgagg
      361 agcttcttcg cacaccgtct ttccgcatgg aacggatcgt ctcatccggc catgcgtcgc
      421 cgccgggctt ttggtacgat caggaggagg acgagtgggt ggcgctgctc cagggcgagg
      481 ccgcgctgga gttcgaggac ggctccgccg ataagctccg cagcggcgac tgggtctttc
      541 ttccggcgca catgcgccac cgagtggcgt cgacgagctt ggaaccgccc tgcatctggc
      601 tggctgtttt tgcccgcccg ggataactat cgaagtgaac ggtgaaaatc gcatctccga
      661 agagtttgcg atctgtacat tcttaaaaaa taaaaattgt atttttgtgt tctagatctc
      721 tccatatcaa gcggaatcct ctatgccacg cgaaaatttt gtgattttta atgtaattcg
      781 tgtatgattg taacgagcct cctcatagat gttgcctctt catcaggaag ggtgagttcg
      841 atgattcgaa gaacgctttt gttcgtgcac cttttctttt tcatctacgg cgcttctttt
      901 gcagcggctc tacctatcga tgaatttatt gaggtatgcg gcgccggttc tcttgaggaa
      961 gtgcatgagg ccgtcgcttc aggggccgat gtaaatgcaa cggatgcgga tggatggacc
     1021 gctttgatgg cggcggcgga caacggaaat accgaggtcg ttgcattttt ggttgaccgc
     1081 ggggcggata ttcatagaaa agacagatgc ggaacaacag cgcttatggc ggctgccggc
     1141 ggttcggata cgcgttcggt acaactcctt gtaaatgcag ggggaaacgt caatgaacgg
     1201 aactcgggag ggtggacgcc gcttttcttt gcagcccgtt tcagcgagga acccgacgtg
     1261 atcgcgcttc tgattgcatc gggcgcggaa gtcgacgtag ctgaagcaga cggtgagacg
     1321 cctcttatga tcgcggcatg gtacggcgag aatccgcgta ttgtggcggc tttgctgaag
     1381 ggcggggccg acgtgcaccg gaaggatact aaagggtgga cgcctctttc atacgccgcg
     1441 tgcacaaata aaaatccgga agttattgca gcgctgttgc gaggaggggc ggatgtcgac
     1501 gaacgggatg aagacgacgg aacgacgcct ctgatgaatg ccgctgcgtg caaccggaat
     1561 cctgcggtac tttcgacgct cctgagggcg ggcgccgatg tgaatgcaaa agatacgaaa
     1621 gggtgtactc ctttgcactt tgcggcgagg tacgccgaga atcccgaagt tgcccgaata
     1681 ttgctggacg cgggagcgga cgtagccgcg cgggacgcgc aatgggggcg gacgcctctt
     1741 ctatgggcgg ctaaaacttc gaaggagccg aagattgtcg aggcgctttt ggatgccgga
     1801 gcggatattc acgcgcggga taactacggg cgcactgcgc tcatgtgcgc ggccgaagag
     1861 tgctgttcac atcctgatgt cgtccgcgcg ctcttgcgtg gaggggccga tgtgaatgcg
     1921 ctgacttccg acgggcggag cgctcttttt ttcgcggcgg ctttccaaca caatgataca
     1981 atcgttcacc ttctgttgaa ggagggggcg gatgtgaata tacgggatac ggcgggagta
     2041 acgtctcttt tgtttgctgc tgagtacggt accaggccgg agattctcct tgcgttgatc
     2101 cacaacggag ccgatgcgag cgtttgcgat gcggacggaa agcgtgcagt cgactacgcg
     2161 gagggaaaca gctatctgaa ggacaccgag gttgtacagg ttttaaaaaa agtttcaccg
     2221 caataagacg gtcgtatcat ggaaacgtac actggctgaa aaatgagtat cgggaggaga
     2281 aaactatgag aataatgatg agacgtgttg cagctattct tttcctttgt gtcgcaagtc
     2341 tctggtgtag ctgttccgcg cacgcgaacg cggttgttct gctcgacacg ccggaggccg
     2401 ccatggatct gaccgagcga atcatggaaa aggtcgccga tgataatatc gagagcggta
     2461 tttttctgat gcggcctttt ttccgccttc ccaaggaaga gttcaacata cttctggaac
     2521 agagtaaatt acaaattcct gtgatcaaag accgtttcgg agctccattg gaacacgaac
     2581 tcattgaaca acgtttcata ggaaaatcgt tgatgcggat cgtcc
//
LOCUS       DAOTIL010000344         2621 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminobacteriaceae bacterium isolate AMR_MDS_3026
            NODE_46570_length_2621_cov_2.643414, whole genome shotgun sequence.
ACCESSION   DAOTIL010000344 DAOTIL010000000
VERSION     DAOTIL010000344.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868050
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminobacteriaceae bacterium (wastewater metagenome)
  ORGANISM  Aminobacteriaceae bacterium
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae.
REFERENCE   1  (bases 1 to 2621)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2621)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:19:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,224
            CDSs (total)                      :: 3,198
            Genes (coding)                    :: 3,184
            CDSs (with protein)               :: 3,184
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2621
                     /organism="Aminobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_46570_length_2621_cov_2.643414"
                     /isolate="AMR_MDS_3026"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:3034609"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..910)
                     /locus_tag="P5201_07775"
     CDS             complement(<1..910)
                     /locus_tag="P5201_07775"
                     /inference="COORDINATES: protein motif:HMM:NF013015.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Y-family DNA polymerase"
                     /protein_id="HRV98471.1"
                     /translation="MGHPGAFLKPCEELLALCDCNNFFVSCERLFRPDLEGRPVVVLS
                     SNDGVIISRSNEAKTLGIKMGEPYFRVRAFLERRGGAVFSSNFQLYEEISARVMEVLR
                     RSTPSIEVYSVDEAFLELPPAARERPVEWGRELRAAVLKETGIPVSVGVAPAKTAAKL
                     AAERGKKRPETGGVFALLPGEAWDDILRDVPVEDVWGIGRQWSDFLRRRGVSTALRLR
                     DTPDDWLEKYLGVRGLRTAWELRGIRCFPIEEYEKPQKSIHSSRSFSTPVRSRESLAE
                     AVTEFALTAGRRLRSQRSLAGCLSVSI"
     gene            complement(886..1161)
                     /locus_tag="P5201_07780"
     CDS             complement(886..1161)
                     /locus_tag="P5201_07780"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum ABC transporter permease"
                     /protein_id="HRV98472.1"
                     /translation="MAVHMTRSNEIQGSAAGRLLEMLLPRPTSTFLLRCADGPDSILV
                     VDRSLPPSEGKKAVLSTEKGLRLATLGSAFDSSSVWGVVTWVIREPS"
     gene            1551..>2621
                     /locus_tag="P5201_07785"
     CDS             1551..>2621
                     /locus_tag="P5201_07785"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRV98473.1"
                     /translation="MKNMIVVVCALLAFGAFLVSARPANAGEPSVSVDYEYGDLKEVI
                     VGLPYGMSPSLEAKWFADALKVLPDDEAEYARKTAGMLWTEMINPETGKSETQMLEEE
                     NQAFIAILESLGVKVYRPAEITVGFIKKHYGADVLLNGFSQDFPRDNMAVIGNNVIEF
                     NLRTAIRKVDISGFRDILSEKCSDSTVKWFSMPHTELLEPARADTPFLEGGDVIVLGK
                     TVFVGNTENPSVGSNEAGYKWLKNIVGDRYTVKRIRLVESVLHLDCVLSVPRSGLAIV
                     CEEAFIDGLPEEIKDWDLIRVSLDDVKRLAVNGLPVDSQNYVLSYNLHNDNRFIQTEL
                     ERRGIKVHRVFFGTHNGQGGSLR"
BASE COUNT          557 a          759 c          798 g          507 t
ORIGIN      
        1 agatgctcac ggagaggcat cccgccaggg agcgctggga gcggagccgc cgccccgcag
       61 taagggcgaa ctccgtcacc gcctcggcga gagattcccg gctccggacg ggagtggaga
      121 aggagcggga ggagtgtatg gacttctggg gtttttcata ttcttctatg ggaaaacagc
      181 gtatcccccg cagctcccac gccgtccgca acccccggac gccgagatac ttctccagcc
      241 agtcgtcggg cgtgtcccgc aggcgaaggg ccgtggacac gccccgtctg cggaggaagt
      301 cgctccactg ccgcccgatc ccccacacgt cctccacggg aacatcccgg agaatgtcgt
      361 cccatgcctc tcccggcaga agggcgaaga ccccgcccgt ctccggccgc ttcttgcccc
      421 gttcggcggc cagcttcgcc gccgtcttcg cgggagcgac gcccaccgag acgggaatcc
      481 ccgtctcttt cagcacggcg gcccggagtt cgcgccccca ttcgaccggg cgttccctcg
      541 ctgcgggcgg aagctccaga aacgcctcgt ccacggaata gacctcgatg gacggcgtgc
      601 tccgccggag gacctccatg accctggccg atatctcctc gtagagctga aagttgctgg
      661 aaaacaccgc gcccccgcgc cgctccagaa aggcgcgcac ccgaaagtag ggctccccca
      721 ttttgatacc gagagtcttc gcctcgttgg agcgggagat gatcaccccg tcgttgctgg
      781 agagcaccac cacgggacgc ccctcaaggt cggggcggaa gagccgttcg cacgagacga
      841 agaagttatt gcagtcgcag agggcgagca gctcctcgca aggcttcagg aaggctcccg
      901 gatgacccat gtcaccaccc cccacacgct ggacgaatcg aacgcggagc ccaatgtggc
      961 gagcctgagc cccttctccg tggagaggac ggccttcttc ccctccgaag gggggagcga
     1021 gcggtccacc acgaggatgg agtcgggccc gtcggcgcac cgcagaagaa atgtggaggt
     1081 cggccgggga agcagcatct ccagcaggcg accggccgcg ctgccctgaa tctcgttgct
     1141 tcgcgtcatg tgaacggcca tagaatgcac tctccttcag actgtttttg tgtataatga
     1201 ttacagtgag tctttgcgtg tcagtataat aatatacaca tagaaagtca atacctttta
     1261 tagatttctt aaagtatata tttgagagcc tgtcgcggag gaaacgctgg ttgagagtat
     1321 tcacagacgg aagtgcgttc cgagagggta tgacgctgta aagactgctg cgggcggatc
     1381 attgcggtgt ataatttcaa aagcagcggc agcgctccga tgtatctgtt gccgccggac
     1441 atgagaaagc ggctgccgga acatcatcgt gtcctgtgta tgcgccgggg agttgaggcg
     1501 tgccgatatg aaggaaggaa agattttttc cactcacgag gagagatgtc atgaaaaata
     1561 tgatcgtggt tgtatgtgcg ctcctggcgt tcggagcgtt tctggtttcc gcccgacccg
     1621 caaacgcggg ggaaccttcc gtttccgtgg attacgaata cggcgatctg aaagaggtga
     1681 ttgtcggcct gccgtacggc atgtcgccgt cgctggaagc caaatggttt gccgacgcgt
     1741 tgaaagtgct gccggacgat gaggcggagt acgcccggaa gacggcgggg atgctctgga
     1801 cggagatgat caatcccgaa acggggaaaa gcgagacgca gatgctggaa gaggaaaatc
     1861 aggcgttcat cgcaatttta gagtctctgg gcgtcaaagt gtaccgtccg gcggagatta
     1921 ccgtcggctt catcaaaaag cattacggcg ccgatgtact gctcaacggt ttcagccagg
     1981 attttccccg cgacaacatg gccgtgatcg gcaacaacgt catcgagttc aatttgcgga
     2041 cggcaatccg caaggtggat atatccggat tccgggatat tctgtccgaa aagtgttccg
     2101 actcgacggt caagtggttt tccatgccgc acacggagct tctcgaaccc gcccgcgcgg
     2161 atacgccgtt tcttgagggc ggcgacgtca tcgtgctggg taaaaccgtt ttcgtcggca
     2221 atacggaaaa tcccagcgtg ggatccaacg aggccggcta caagtggctc aaaaacattg
     2281 tgggcgatcg ctatacggtg aaacgcatcc ggctcgtcga aagcgtgctg catctggatt
     2341 gcgtgctttc cgtgccgcgc agcgggcttg ccatcgtctg cgaagaggcc ttcatcgacg
     2401 gcttgcccga agagatcaag gactgggacc tgatcagagt gagtctggac gacgtcaagc
     2461 gactggccgt caacgggctt ccggtcgatt cccaaaacta cgttctgtcg tataacctgc
     2521 acaatgacaa ccgtttcatc cagaccgagc tcgaaaggcg gggcatcaaa gtccaccgcg
     2581 tatttttcgg aacccacaac ggccagggtg gatcgttgcg c
//
LOCUS       DAOTIL010000345         2620 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminobacteriaceae bacterium isolate AMR_MDS_3026
            NODE_46598_length_2620_cov_2.806238, whole genome shotgun sequence.
ACCESSION   DAOTIL010000345 DAOTIL010000000
VERSION     DAOTIL010000345.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868050
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminobacteriaceae bacterium (wastewater metagenome)
  ORGANISM  Aminobacteriaceae bacterium
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae.
REFERENCE   1  (bases 1 to 2620)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2620)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:19:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,224
            CDSs (total)                      :: 3,198
            Genes (coding)                    :: 3,184
            CDSs (with protein)               :: 3,184
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2620
                     /organism="Aminobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_46598_length_2620_cov_2.806238"
                     /isolate="AMR_MDS_3026"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:3034609"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1514)
                     /locus_tag="P5201_07790"
     CDS             complement(<1..1514)
                     /locus_tag="P5201_07790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015555934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HRV98474.1"
                     /translation="MRKGIAAVLGIALLLSLSGAAALAAPREVVIGMPGDVHSMDPYT
                     LNETINNAINNHLFDTLVETDNNLQPKPGLAESWEVSEDARTWTFHLRKGVTFHNGNP
                     FTVDDVIYSFDRSKDREVGASFVHVLSTVESYEKIDDHTLKVTCKSPNALLLAHFKDL
                     LILNKAHCEGQGNDWIALNPNGTGRYKLVEHVRGDRLVLERNPSFWGEQPIVEKVTFK
                     PITNEGTRTANMLSGAVDLVVNVPVRDVKMLEKNKNVTVIAQPGLRAIYLNMAGWSDT
                     PSKDAKMPLKSPDGSNPFKDIRVREAMYRAINIDEIVEKIMNGFAKPMATYSPEGYNG
                     FNPDVKRLAYDPALAEKLLDEAGYKRQSDGYRFEVTLDAPNDRYINDGPVATAIAGYF
                     EKVGIKVNPNLMSRSVFFSYISTTNKAGDNTHLCMTGWTDSGGESALIGLDMLYSITQ
                     NGEVKPGYGGVNRGFYCNPEVDKLIDEAIATGDPAQRGEIMKKAWQLAADDVSY"
     gene            complement(1628..>2620)
                     /locus_tag="P5201_07795"
     CDS             complement(1628..>2620)
                     /locus_tag="P5201_07795"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HRV98475.1"
                     /translation="MLTSLGIRGDARKFAEAGFSGYLNKPVRQHDLRDVLSFILTGDA
                     DAPGAIVTRHTVRDALSGFDGSGARILLAEDNITNQHVALGILKKFGLRADAVADGEE
                     ALAALSAVPYDLVLMDCQMPRMDGYEAARRVRMMEGAVRDVPIIAMTASAMAGDREKC
                     LEAGMNDYVPKPVSPGVLAGALKKWLPSRGERGGAREASSKESAANGSPVLQSKEQNA
                     ESRPWNREAFLERLRGDHELAAEIVGDYLSEIPKEIDALREALATGDAEAGERCAHSV
                     KGVSASVGGERVRDVAFRMERAAAAGDLEMARASLPRLEEEFELLKDAMKRSGH"
BASE COUNT          418 a          808 c          815 g          579 t
ORIGIN      
        1 atgtaggaga cgtcgtcggc ggccagctgc caggccttct tcatgatttc gccgcgctgc
       61 gccggatcgc cggtggcgat cgcctcgtcg atcagcttgt cgacctcggg gttgcagtag
      121 aagcccctgt tgacgccgcc gtaccccggt ttgacctcgc cgttctgagt aatgctgtag
      181 agcatgtcga gcccgatgag cgcgctctcg ccgcccgagt cggtccagcc ggtcatgcag
      241 aggtgcgtgt tgtcccccgc cttgttggtg gtggagatgt aggagaaaaa gaccgagcgg
      301 gacatgaggt tcgggttgac cttgatgccg accttctcga agtagcccgc gatcgccgtg
      361 gccacgggac cgtcgttgat gtagcggtcg ttgggagcgt cgagggtgac ttcaaaacgg
      421 tagccgtcgc tctgccgctt gtatccggcc tcgtcgagca gcttctccgc gagcgcgggg
      481 tcgtacgcaa ggcgcttcac atccggattg aatccgttgt acccttcggg cgagtaggtg
      541 gccatcggct tcgcgaagcc gttcatgatc ttctcgacga tctcgtcgat gttgatggcg
      601 cggtacatcg cctcgcggac gcggatatcc ttgaacggat tgctcccgtc gggggacttc
      661 agcggcatct tcgcatcctt cgacggcgtg tcgctccacc ccgccatatt gaggtagatg
      721 gcgcgcagtc cgggctgagc gatgacggtg acgttcttgt tcttctccag catcttcacg
      781 tcgcggacgg ggacgttgac gaccaggtcg accgcgccgg agagcatgtt ggcggttcgt
      841 gttccctcgt tggtgatcgg cttgaacgtc accttttcga cgatcggctg ctctccccag
      901 aaggaggggt tgcgctccag cacgagcctg tcgccgcgca cgtgctccac cagcttgtag
      961 cggccggtgc cgttggggtt caacgcgatc cagtcgttcc cctggccttc gcagtgcgcc
     1021 ttgttcagga tcagcagatc cttgaagtgc gccagcagca aagcgttggg cgatttgcag
     1081 gtgactttca gcgtatggtc gtcgatcttc tcgtagctct ccactgtgga gagcacgtgc
     1141 acgaaggaag cgccgacctc tctgtccttg gaacggtcga aggagtagat gacgtcgtcg
     1201 acggtgaagg ggttgccgtt atggaaggta actccctttc tgaggtggaa ggtccaggtg
     1261 cgggcgtctt cagagacttc ccagctctcg gcaaggccgg gcttgggctg gagattgttg
     1321 tccgtctcga ccagcgtgtc gaagagatga ttgttgatgg cgttgttgat cgtttcgttc
     1381 agcgtgtagg ggtccatcga gtgaacatcc cccggcatgc cgatcacgac ctcccgtggc
     1441 gctgcgagcg cggccgctcc cgacaacgag agcagcaggg cgattccgag tactgcggcg
     1501 attcctttcc tcacgataca ttcctccctt ttctgtggtg acattccgaa tgcaaggcta
     1561 agtatacctt tgcggataca ttttatagtg ctccgaaggc aaagtcaatc gcactttttc
     1621 gacagtttca gtgtcccgat cgtttcatcg cgtctttcag cagttcgaat tcttcttcca
     1681 ggcgaggcaa agacgctctt gccatctcca ggtcgcccgc agccgcggcc ctctccatgc
     1741 ggaaggctac atcgcggact ctttcgccgc cgacgctggc ggagacgcct ttcacgctgt
     1801 gcgcacagcg ctctccggct tccgcatctc ccgttgcgag ggcctcccgg agcgcgtcga
     1861 tctccttcgg aatctcggag agataatcgc ccacgatctc cgccgccagc tcgtggtctc
     1921 ctcgcaggcg ttccaggaag gcctccctgt tccacggacg cgactcggca ttctgctcct
     1981 tgctttgcag tacgggagag ccgttcgccg cgctctcttt tgacgaagcc tcccgggcgc
     2041 cgccgcgttc accgcgtgac ggcagccatt tcttcagcgc tccggcaagg acgcccggag
     2101 aaaccggttt tggaacgtag tcgttcatcc cggcctccag gcacttttcc ctgtcccccg
     2161 ccatggcgct cgccgtcatg gcgatgatcg gcacgtcacg aaccgccccc tccatcatgc
     2221 ggactctccg cgcggcttcg tacccgtcca tgcgcggcat ctggcaatcc atcaggacga
     2281 gatcgtacgg tacggccgag agggcggcaa gcgcctcttc tccgtcagcg accgcgtccg
     2341 ctctcaggcc gaacttcttg agtatgccga gggcgacgtg ctggttggtg atgttgtcct
     2401 cggcgaggag gatgcgcgcg ccgctgccgt cgaagcctga aagggcgtcg cggaccgtgt
     2461 ggcgggtgac gatggcgccg ggagcgtccg catctccggt caggatgaaa gagagcacat
     2521 cccgaaggtc gtgctgacgg acaggcttgt tcaggtagcc ggagaagccc gcctcggcga
     2581 acttcctggc gtccccgcgt attccgagcg acgtgagcat
//
LOCUS       DAOTIL010000346         2616 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminobacteriaceae bacterium isolate AMR_MDS_3026
            NODE_46693_length_2616_cov_3.293635, whole genome shotgun sequence.
ACCESSION   DAOTIL010000346 DAOTIL010000000
VERSION     DAOTIL010000346.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868050
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminobacteriaceae bacterium (wastewater metagenome)
  ORGANISM  Aminobacteriaceae bacterium
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae.
REFERENCE   1  (bases 1 to 2616)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2616)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:19:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,224
            CDSs (total)                      :: 3,198
            Genes (coding)                    :: 3,184
            CDSs (with protein)               :: 3,184
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2616
                     /organism="Aminobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_46693_length_2616_cov_3.293635"
                     /isolate="AMR_MDS_3026"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:3034609"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(175..1455)
                     /locus_tag="P5201_07800"
     CDS             complement(175..1455)
                     /locus_tag="P5201_07800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009200904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0031317 - tripartite ATP-independent
                     periplasmic transporter complex [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter large permease"
                     /protein_id="HRV98476.1"
                     /translation="MLLMMIGVMCFFLVLGFPMIIPLIIAPIAVAAVYYPALDPMNLV
                     QQMLVGVRPLSLVAVPMFIFAADVISTGQVARRLIDFVMAFFGHTRGGLAVTTTAACT
                     FFGAVSGSTQATVVAIGGTMRPYLLRDGYDDSFSIALIINAAGIALLIPPSISMIIYG
                     VVTGSSVGELFIAGIGPGLLVFLLFSLYCSFAARKMDIVRTPKMPLPERVKALRKAIV
                     PIGFPLIILGGIYSGKFSPTEAAAVAVLYAVVVEIFLYRHIGWRDLYRIALSTGIVTA
                     VVFILVAVGASFSWLVGYARIPEAILPPILGESPSMIKLLLVIAASYFIGCMFVDSIV
                     VIMILAPIFHPIATKLGIDPILIGVLVTMQAAIGAVTPPFGCNIFTAMAVFRRPYADV
                     VRRIMPFLALYILATLIVIFFPSLSLFLRDMAFR"
     gene            complement(1457..2023)
                     /locus_tag="P5201_07805"
     CDS             complement(1457..2023)
                     /locus_tag="P5201_07805"
                     /inference="COORDINATES: protein motif:HMM:NF016200.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter small permease"
                     /protein_id="HRV98477.1"
                     /translation="MKKLELKGLEKIDAVLTWVEERILVFSILFMAVILIANVISRNF
                     FNSSINASEEISQFLVVLATFLGTSYAARKGLHIRMSILNDFLKGKPQKMLALFVSLA
                     TAVIMFYISWLAFRYVRRVGTLHRVSPILQVPLHYIWSVVPVGFLLTGVQYLLTFARN
                     LTSGEVWVSYYVKDENAEPEAAPAEGGN"
     gene            complement(2117..>2616)
                     /locus_tag="P5201_07810"
     CDS             complement(2117..>2616)
                     /locus_tag="P5201_07810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009200900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRV98478.1"
                     /translation="VYDEGALKLVNQMRAEGFLPKETIFKWSVFGGQCSPAGAKLLRE
                     LGADTMNPISDVSLPILSSIRSTVDMPLDVYMIIVDSFGGMFRAYDAPEIVRVASPVY
                     FKIEPGTSEGDIYKPWVTDSWHDEFIRAKVKIAATLREIMERRAPELKLSEQGPADLR
                     LPVVD"
BASE COUNT          530 a          755 c          903 g          428 t
ORIGIN      
        1 caggcacctc cttcagattg agaacacgtt gtttccgtac agcgtttcct gaacccgacg
       61 cctcttgagg cgtcgaaatc atgcgcgccc ggcggcgtca agaaatccgc tccgccgcgc
      121 ccttcctctg catgtttcct gcgaacgccc cctttcgtcc cgtcgttccc gccgctaccg
      181 gaacgccatg tccctcagga agagggagag cgaggggaag aagatgacga tgagcgtcgc
      241 caggatgtag agcgcgagga agggcatgat gcgcctgacg acatccgcat acggtcgtct
      301 gaagaccgcc atcgcggtga agatgttgca gccgaagggc ggcgtcaccg cgccgatggc
      361 cgcctgcatg gtgacgagca cgccgatgag gatcgggtcg atgcccagct tcgtcgcgat
      421 cgggtggaag attggcgcga ggatcatgat gaccacgatg gaatcgacga acatgcagcc
      481 gatgaagtag gaggccgcga tgacgagcag cagcttgatc atggaggggc tctcccccag
      541 gatcggcggg aggatggcct ccgggatgcg cgcgtagccg acgagccacg agaaggaggc
      601 gccgacggcc acgaggatga agacgacggc ggtgacgatg ccggtggaga gggcgatccg
      661 gtagagatcc ctccagccga tgtgccggta gaggaagatc tccacgacca ctgcgtaaag
      721 gacggcgacg gccgcggcct cggtcggact gaactttccg gagtaaatgc cgccgaggat
      781 gatcagcggg aagccgatgg ggacgatcgc cttccgcagc gccttcaccc gctcggggag
      841 cggcatcttc ggcgtacgga cgatgtccat cttccgcgcc gcgaagctgc agtagagcga
      901 gaagaggagg aagacgagca gccccgggcc gatgccggcg atgaagagtt cgccgacgga
      961 gcttcccgtg accacgccgt agatgatcat cgagatgctc ggggggatga gcagcgcgat
     1021 tcccgccgcg ttgatgatga gcgcgatgga gaacgagtcg tcgtacccgt cgcggagcag
     1081 gtaggggcgc atggtgccgc cgatggcgac gaccgtggcc tgcgtggagc cggagaccgc
     1141 gccgaagaag gtgcacgccg ccgtggtggt gacggcgagg ccgccgcgcg tgtggccgaa
     1201 gaaggccatg acgaagtcga tgagccgccg cgccacctgc cccgtggaga tgacgtccgc
     1261 ggcgaagatg aacatcggga cggcgaccag agagagcggc cgaacgccga cgagcatctg
     1321 ctggacgagg ttcatcgggt cgagcgccgg gtagtagacc gcggccacgg cgatgggggc
     1381 gatgatcagg gggatgatca tcgggaagcc gaggacaagg aagaagcaca taacgccgat
     1441 catcatgagg agcatctcag tttcccccct ctgccggagc cgcctcgggc tccgcgttct
     1501 cgtccttcac gtagtaggag acccagacct cgccgctcgt caggtttctg gcgaaggtca
     1561 ggagatactg cacgccggtg aggaggaagc cgacgggaac caccgaccag atgtagtgga
     1621 gaggaacctg gagtatgggg ctgacgcgat ggagcgtccc caccctgcgc acgtagcgga
     1681 aggcgagcca cgagatgtag aacatgatga cggccgttgc cagcgagacg aagagggcga
     1741 gcatcttctg cggcttcccc ttcagaaagt cgttcaggat ggacatccgt atgtggagcc
     1801 ccttcctggc ggcgtagctt gtccccagga aggtcgccag caccacgaga aactggctga
     1861 tctcctccga cgcattgatg ctggagttga agaagttgcg cgagatcacg ttggcgatca
     1921 gtatgaccgc catgaacaga atgctgaaca ccagaatccg ttcttctacc catgtcagca
     1981 cggcgtcgat cttttcgagt cccttcaatt cgagtttctt caatggctga tcctcccttc
     2041 aggtgagcgg gcgcggcccg gaggccgaag gtaaaacagc ccgggaagcg gatactcccg
     2101 ggctgttcgg tagatgtcag tcgacgacgg gcaggcggag gtcggcgggc ccctgttcgg
     2161 agagcttcag ttcgggggcg cggcgttcca tgatctcgcg gagcgtcgcg gcgatcttca
     2221 ccttggcgcg gatgaactcg tcgtgccacg agtcggtcac ccacggcttg tagatgtcgc
     2281 cctcggaggt tcccggctcg atcttgaagt agacggggga ggccacgcgg acgatctcgg
     2341 gcgcgtcgta ggcgcggaac atgccgccga aggagtcgac gatgatcatg tacacgtcga
     2401 gcggcatatc gacggtgctg cggatgctgg aaaggatcgg gagcgagacg tcggagatcg
     2461 ggttcatcgt gtcggccccc agctcccgga ggagcttcgc cccggcggga ctgcactgac
     2521 cgccgaaaac tgaccacttg aaaatggttt ctttcgggag gaagccctcg gcgcgcatct
     2581 ggttcaccag cttcaacgcc ccttcgtcgt agacga
//
LOCUS       DAOTIL010000347         2610 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminobacteriaceae bacterium isolate AMR_MDS_3026
            NODE_46834_length_2610_cov_3.178865, whole genome shotgun sequence.
ACCESSION   DAOTIL010000347 DAOTIL010000000
VERSION     DAOTIL010000347.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868050
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminobacteriaceae bacterium (wastewater metagenome)
  ORGANISM  Aminobacteriaceae bacterium
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae.
REFERENCE   1  (bases 1 to 2610)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2610)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:19:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,224
            CDSs (total)                      :: 3,198
            Genes (coding)                    :: 3,184
            CDSs (with protein)               :: 3,184
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2610
                     /organism="Aminobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_46834_length_2610_cov_3.178865"
                     /isolate="AMR_MDS_3026"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:3034609"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1149
                     /locus_tag="P5201_07815"
     CDS             <1..1149
                     /locus_tag="P5201_07815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006300388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HRV98479.1"
                     /translation="AERLLFEKLEEKRKNWRSGRNHSQPVITGEDIADVVAEWTGIPV
                     FQITGEESLRLLGMEREIEKRLVGQSEAVASVARAIRRARSGLKDPKRPIGSFLLLGP
                     TGVGKTELARRLASFLFGTDDSMIRLDMSEFMEKHEVSKLIGAPPGYVGYEDGGKLTE
                     AVRRRPYSVVLFDEIEKAHPDIFNLLLQILEDGRLTDGHGRTVDFRNTVILMTSNVGA
                     RDAAKGSPLGFAGSGNDDPFDWSRLKSTILDEVNRTFRPEFINRVDESIVFRPLGRES
                     VGAIVDIMFADVCSRLSGQEVTLTVGEEVKNLVCEKGYQPKFGARPLRRTIQALVEDP
                     LADYLLENGASKGLHVEAFVSEGHVTFSVVPAPVVESSEDRARETVHQ"
     gene            1189..2016
                     /locus_tag="P5201_07820"
     CDS             1189..2016
                     /locus_tag="P5201_07820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014807364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease proteolytic subunit"
                     /protein_id="HRV98480.1"
                     /translation="MDGSSLWFYFLLFFVVFPYLKQQSLRWRRFTALKNCEKRRRSRI
                     ITLIHRQESMGFWGMFSRNFINIEDSEEVLRAIRLTSDDTPIDLIIHTPGGLLLAAEQ
                     IARALKHHPAKVTVIVPHYAMSGGTLIALAADEILMDRHAVLGPVDPQIGQYPAASIL
                     KAVEEKPVSEVDDNTLILADISRKAIRQTKNFVFELLSERMGEEKGMELAEALTEGRW
                     THDYPIGVDEATALGLPVVLNDYDSFCNIMGLYHQSVTERPSVQYIPVPYPGRPSGN"
     gene            2034..>2610
                     /locus_tag="P5201_07825"
     CDS             2034..>2610
                     /locus_tag="P5201_07825"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRV98481.1"
                     /translation="MSKSEKWVFSLLFLSLVLIHPLAAAAAPGNEIVLTVDDEQVSVD
                     EILYLLALQSGAGEAAAPLLAAQMTPTEREAFLEQVSRAILFSKGAIIRGLHLDPGVS
                     ARIRWSRINTLAEAYVGSMASSLSFEEKTLRAHFEKNKQKYRQNESVRVRHVFTTEEE
                     NARTALLRILSGEVFAEVAASLSVDYATSETG"
BASE COUNT          602 a          644 c          740 g          624 t
ORIGIN      
        1 gccgagcgtc tgcttttcga aaagctggaa gagaaacgga aaaactggag gtcggggaga
       61 aatcattctc agccagtgat cacaggtgaa gatattgcgg acgtagttgc agaatggacc
      121 ggcatcccgg tgtttcagat cacaggggaa gagtctttgc gtcttcttgg aatggaacgc
      181 gagatagaaa agaggctcgt aggacagagc gaagctgtcg cgtcggttgc gagggctatc
      241 aggcgcgcca gaagcggtct gaaggacccg aagcggccca ttggaagctt ccttctgctt
      301 gggcctacag gagtgggcaa gactgagctg gcgagacgcc tggcatcctt cctcttcggg
      361 accgacgatt caatgattcg tctcgatatg agcgagttca tggagaaaca tgaagtctca
      421 aagcttattg gcgctcctcc cgggtacgtt gggtacgaag atggggggaa actcacggaa
      481 gcggtgcggc gccgccccta ttccgtggtg cttttcgatg aaattgaaaa agcgcatccc
      541 gacatattca acctgcttct ccagatactg gaagacggcc gattgactga cgggcacgga
      601 cgaacagtgg atttccgcaa taccgtaatc ctgatgacca gtaacgtcgg cgccagagat
      661 gccgcgaagg ggtctcctct tggattcgcc ggatcgggaa acgacgatcc gttcgactgg
      721 agccgtctta aaagcactat ccttgacgaa gtgaaccgaa ccttccgtcc cgaattcata
      781 aatcgtgtcg acgagagtat tgtcttccgc cccttaggaa gagagagcgt cggcgcaatt
      841 gttgatatta tgtttgcaga cgtctgttcg cgattgtcgg gccaggaggt cacgctgaca
      901 gtgggagaag aagtgaagaa cttagtgtgc gagaaagggt atcagccaaa gttcggggcc
      961 cggcctctca gaaggacgat acaagcgctt gtcgaagacc ccttggctga ttatcttctt
     1021 gaaaacgggg cttcaaaggg gttgcacgtc gaggcgtttg tttccgaggg acatgtcact
     1081 ttcagcgtcg tccctgcgcc cgttgtagag tcttcggagg atcgtgcgcg tgaaactgtg
     1141 catcagtagt tattcgcttg tgctcgttgt ttctggaagg aggctgtcat ggacggatca
     1201 tcgctttggt tttacttcct gctctttttt gtagtctttc cttacttaaa gcagcagtcg
     1261 cttcggtgga ggcgttttac tgcgctcaaa aactgtgaaa agagacgccg ttcacggatt
     1321 ataactttga ttcaccgaca ggagagcatg ggattctggg ggatgttctc ccggaacttt
     1381 atcaatattg aggactccga ggaggttctt cgagctattc gcttgacctc ggacgatacc
     1441 cccatagacc tgatcataca tacgccaggc ggtctccttc tcgcagcgga acagattgca
     1501 cgcgctttga agcatcatcc ggcaaaggtt actgtgatcg tcccgcatta cgccatgtca
     1561 ggcgggacgc ttatcgctct cgcagccgac gaaatcctga tggatcgcca cgcggttctc
     1621 ggtcctgtgg atcctcagat agggcagtac ccggcggcct ctatactgaa agcagtagaa
     1681 gaaaaacccg tcagcgaggt tgatgacaat acgctgatac ttgccgatat ctcccgaaag
     1741 gcgatccgcc agacgaagaa cttcgttttt gagctccttt ccgaaagaat gggtgaagag
     1801 aaggggatgg agctcgcgga ggctctcacg gaaggccgat ggactcatga ctacccgata
     1861 ggagtagacg aagcgaccgc tctcgggctt ccggttgtgc tcaatgatta tgactccttc
     1921 tgcaacatca tggggttgta ccatcagtcc gttaccgaga ggccttccgt ccaatatatt
     1981 cccgtcccct acccgggacg cccttccgga aattgaaagg cgggtgtgtt cgaatgagca
     2041 agtcggaaaa gtgggttttc agtctccttt ttctttctct cgtactaatt catccgcttg
     2101 ccgcagcggc agcgccgggg aatgaaattg tccttactgt tgatgacgag caagtttctg
     2161 ttgatgaaat actctatctt ttagctcttc agtcaggggc cggcgaagcc gctgcccctc
     2221 tgcttgcagc gcagatgact ccaaccgaga gagaggcgtt tcttgaacag gtttcgcggg
     2281 cgattctctt ctcgaagggg gcaattatcc gaggattgca tcttgatcct ggagtttcgg
     2341 cccgtatccg ttggagccgc atcaatacgc ttgccgaggc gtatgtcggc agtatggcct
     2401 cttcgctctc ttttgaagag aaaaccctga gagctcattt tgaaaagaat aaacagaaat
     2461 accgacagaa tgaatcagtc cgggtcaggc atgtattcac cacagaagag gagaatgcgc
     2521 gaacagcgct tctccgcatt ctctcgggag aagtttttgc cgaagtcgct gcatcgctca
     2581 gcgttgatta cgccacgtcc gaaacaggag
//
LOCUS       DAOTIL010000348         2610 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminobacteriaceae bacterium isolate AMR_MDS_3026
            NODE_46839_length_2610_cov_2.455186, whole genome shotgun sequence.
ACCESSION   DAOTIL010000348 DAOTIL010000000
VERSION     DAOTIL010000348.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868050
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminobacteriaceae bacterium (wastewater metagenome)
  ORGANISM  Aminobacteriaceae bacterium
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae.
REFERENCE   1  (bases 1 to 2610)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2610)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:19:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,224
            CDSs (total)                      :: 3,198
            Genes (coding)                    :: 3,184
            CDSs (with protein)               :: 3,184
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2610
                     /organism="Aminobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_46839_length_2610_cov_2.455186"
                     /isolate="AMR_MDS_3026"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:3034609"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..188
                     /locus_tag="P5201_07830"
     CDS             <1..188
                     /locus_tag="P5201_07830"
                     /inference="COORDINATES: protein motif:HMM:NF016136.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelB/DinJ family
                     antitoxin"
                     /protein_id="HRV98482.1"
                     /translation="KVGLTPTEAIRLFYRQTINTGGIPFGLVLPEETLAAMREAESGT
                     AEEISLDDLKAELDAIC"
     gene            175..465
                     /locus_tag="P5201_07835"
     CDS             175..465
                     /locus_tag="P5201_07835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011114071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system YafQ family
                     toxin"
                     /protein_id="HRV98483.1"
                     /translation="MRSVRQTRQTAAFKRDFKREIKRASRHILKERLWGVVAALTMGS
                     PLDPSYRDHPLGGNWRGHRECHIAPDLLLLYYLSEDALTLVRLGSHAELFGL"
     gene            complement(555..740)
                     /locus_tag="P5201_07840"
                     /pseudo
     CDS             complement(555..740)
                     /locus_tag="P5201_07840"
                     /inference="COORDINATES: protein motif:HMM:NF025120.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="XRE family transcriptional regulator"
     gene            766..2256
                     /locus_tag="P5201_07845"
     CDS             766..2256
                     /locus_tag="P5201_07845"
                     /inference="COORDINATES: protein motif:HMM:NF012686.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:solute symporter family protein"
                     /protein_id="HRV98484.1"
                     /translation="MFSAFPPFLWYYLKGGIRLGHTLERLPPKTIEKVFFVFMLSAGL
                     VLVVFFISGLFSSRGVSSSSDYAVANRKAGVRGVSGVILGSLVGGAATVGTVQMAYQW
                     GLSALWFTLGSGAACLLMGLWFVSPLRSVELITLPQYLGRHFGKRTALLVAVSTASGS
                     FLSVIAQFLSGVALMRSVFPVSTGVAALLTGALILAFVFGGGIKSFSRLGEAKIIFLY
                     MVMLLCSGVVLVQGWTPGAIIEALPSEPFFNILSRGAGKDLGSFASLLCGVLCGQIYI
                     QAVYTASSNATAKKGCLWAAFIIPPMGFLGTWIGLSMRASGVAVEASQALPFFIQTSF
                     HPVLGGLLWSGIMITVLGTAVGVSLGVATNLTRDIFLKMKVAAHFDDVRILLASRVIV
                     LAVVAAAGTVACASRNSMILQWAYLGMGVKASGIFCLMLAAVLRPGRLSHAWSFAAGI
                     GGLLGVAGGAFFGGGLDPLFPGLALSGLVVLVGLSRGRAEALPGDC"
BASE COUNT          394 a          777 c          798 g          641 t
ORIGIN      
        1 ggaaagtcgg tttgactccc acggaggcga ttcgtctctt ttaccggcag accatcaaca
       61 cgggaggcat ccctttcggg ctcgttttac cggaagaaac tctggccgcc atgcgggaag
      121 ctgagtccgg cacggcggaa gaaatatcgc tggatgatct gaaggccgag ctggatgcga
      181 tctgttaggc aaactaggca aacagcggcg ttcaagcgcg attttaaacg ggagatcaag
      241 agggcttcgc gccatattct caaggagcgt ctttggggag tcgtcgccgc gttgacgatg
      301 gggagccctc tcgacccgtc gtatcgcgat cacccgcttg gaggaaactg gcgaggacat
      361 agggagtgtc acatcgcccc ggatctgctg ttgctctatt atctatcgga agatgctctt
      421 accttggtgc gtctcggaag ccatgcggag cttttcggac tttgaagatg acaagcatgt
      481 tgtaaagaac gagtcctcgc tgtcttcttc cttctcggtc tgttctcctc caggggcggt
      541 ttatacgccg acctttattc ggagcggtag cccgagcgat gtcgcaagtt gatcaaggcg
      601 tcgagagaga attcggagag cgttccctcc agtaattcgt ccattcgcgg gcacgtaacg
      661 ctgcaacgac gcgcggcttc ctcgcggttc cacttctgtt tgaggacgaa cgagacgatc
      721 tcgtccataa gttccgctcg gagacgaagg ttggcggcct actgcatgtt ctccgcgttt
      781 cctccgtttc tatggtacta tctcaagggg ggtatccgcc tcggtcatac gcttgagcgg
      841 cttcctccaa aaacaatcga gaaggtgttc ttcgtgttca tgctctccgc aggtcttgtg
      901 ctcgttgtct tcttcatttc cggtctgttc tcctccaggg gcgtctcctc atcctccgat
      961 tacgccgtcg ccaacagaaa agcgggtgtg cgcggcgtct cgggcgtcat cctcggctcg
     1021 ctcgtcggcg gggccgctac ggtcggtacg gtgcagatgg cgtaccagtg ggggctttcg
     1081 gcgctctggt tcaccctcgg cagcggggcc gcgtgcctcc tgatggggct ctggttcgtc
     1141 tcgccgctcc gctcggtgga gctgatcacg ctgccgcagt acctcgggcg gcacttcggc
     1201 aaacggacgg cgctccttgt ggccgtttcc acggcttcgg gctcgttcct ctcggttatc
     1261 gcgcagtttc tctccggcgt ggcgttgatg cgttccgtct tccccgtctc aaccggcgtc
     1321 gcggcgctgc tcaccggagc gctgatcctt gcgttcgtct tcggcggcgg catcaagagt
     1381 ttcagcaggc tcggcgaggc gaagatcatc ttcctctata tggtgatgct cctgtgctcg
     1441 ggcgtggtgc tcgttcaggg ctggactccg ggggcgatca tcgaggcgct tccgtccgaa
     1501 cccttcttca acatcctctc aaggggagcg gggaaggatc tcggctcgtt cgcctcgctg
     1561 ttgtgcggcg tgctctgcgg gcagatctac attcaggctg tctacaccgc atcgtccaac
     1621 gcgacggcga aaaaaggatg cctctgggcg gccttcatca tcccgccgat gggctttctc
     1681 ggcacgtgga tagggctctc catgcgcgct tccggtgtgg ccgtcgaggc ctcgcaggcg
     1741 cttcctttct tcattcagac ctccttccac cccgttctcg gcggtctgct ctggtcgggg
     1801 atcatgatca ccgtcctcgg aacggccgtc ggcgtctcgc tcggcgtcgc gacgaacctc
     1861 acgagggata tcttcctcaa gatgaaggtc gcggcgcact tcgacgatgt gagaatcctg
     1921 ctcgccagcc gcgtcatcgt gctcgccgtg gttgcggcgg cgggaacggt cgcctgcgcc
     1981 tcgcggaatt cgatgattct ccagtgggcc tacctcggga tgggggtcaa ggcctccggt
     2041 atcttctgcc tgatgcttgc cgcggtgctc cggccgggga ggctttcgca tgcgtggagc
     2101 ttcgccgcgg ggatcggcgg tcttctcggc gtcgctgggg gcgcgttctt cggcggcggc
     2161 ctggacccgc tctttccggg gctcgcgctt tcggggctcg tcgtgctggt cggtctgtcg
     2221 agagggcgcg cggaagcgct tccgggggac tgttgaagaa attcgtttcc gcgtctcata
     2281 acgggagtat ctccggtttc tacgcccggg ggcgattcag tgtctgcggg aatgaagcgt
     2341 aatctataat ttgcactact caaaactatt tgactaaaaa gcgcctctgc ggtatcatcc
     2401 gaacaaacgg gtctcttgaa gctgcctgct agggggtgag accgtgttgc cgtggaggcg
     2461 ctttgcttcc ctgtccgtca tgctggcatt tctcctcatt cccagagtcc ttttcgggaa
     2521 cgattcttcc ttcatgtccc gcaatctgtt gcgcacgctt tctctctctc cttccgacaa
     2581 cgcttccgtc tttgttcggc gctcgggcga
//
LOCUS       DAOTIL010000349         2589 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminobacteriaceae bacterium isolate AMR_MDS_3026
            NODE_47342_length_2589_cov_3.451855, whole genome shotgun sequence.
ACCESSION   DAOTIL010000349 DAOTIL010000000
VERSION     DAOTIL010000349.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868050
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminobacteriaceae bacterium (wastewater metagenome)
  ORGANISM  Aminobacteriaceae bacterium
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae.
REFERENCE   1  (bases 1 to 2589)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2589)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:19:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,224
            CDSs (total)                      :: 3,198
            Genes (coding)                    :: 3,184
            CDSs (with protein)               :: 3,184
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2589
                     /organism="Aminobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_47342_length_2589_cov_3.451855"
                     /isolate="AMR_MDS_3026"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:3034609"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..403)
                     /locus_tag="P5201_07850"
     CDS             complement(<1..403)
                     /locus_tag="P5201_07850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005661944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosome-binding GTPase"
                     /protein_id="HRV98485.1"
                     /translation="MNNPFAGIGFRAGNVVLAGRPNVGKSSLVNRLLDYKVSIVSEKP
                     QTTRNVIRCVYNGEDFQIIFSDTPGIHLPKHKLGRTLVDAAVESLEEADLICYVLGGD
                     GSVLRLDDVTDKVAHEDEEILSVLGKVRTPVL"
     gene            complement(400..843)
                     /locus_tag="P5201_07855"
     CDS             complement(400..843)
                     /locus_tag="P5201_07855"
                     /EC_number="3.5.4.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008710277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004126 - cytidine deaminase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0009972 - cytidine deamination [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytidine deaminase"
                     /protein_id="HRV98486.1"
                     /translation="MTLFTSPVSWPASWPSPQKLLDAAREAQRASYSPYSGFPVGAAL
                     FVEGMDDLILACNVENGSYGLSICAERSAAVAMIARGGKKPLAAAVVGKRGEVCLPCG
                     ACRQFLAEFNPEMLLVFEQGDSFVVTTLAELFPAPFLLTEQSGER"
     gene            complement(840..2144)
                     /locus_tag="P5201_07860"
     CDS             complement(840..2144)
                     /locus_tag="P5201_07860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008709435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin family protein"
                     /protein_id="HRV98487.1"
                     /translation="MSSLVAQSAALLLVLLFLSAFFSASETAITSSGRGKILALIERY
                     PYQKRFFEWLLKDVQRALTIVLISNNLVNIAASAVGTTLAIATIGQGGVLLAVPLMTA
                     LIVIFGEVFPKSVAIIQSDFVLVFSLPFLRLLDILLAPFVWLMIAAVRLLGLLFKVDL
                     KARHPFVTREEFEQMVNIGEESGALEEVERRMIHGIISFEETRVYEIMVPRTDMDAVP
                     SNCTVAEAMKVFQEHGHSRVPVFEENLDDIVGILYVKDTIPNLLAGELETPVSSIMRN
                     ALFVPESMRVVELFNNMKGKHVHMAIVVDEYGGVAGIATLEDLLEEIVGEIQDEYDKE
                     KPSFTPEEDGSYLVQGHVSLEDLSDLLEASFESEDAESLGGLVLSISGDFPSPGEKIH
                     YRSPDGAGNWEIEVLEVEDHRIKLLRLLPKERGYFIPPEVVE"
     gene            complement(2141..>2589)
                     /gene="ybeY"
                     /locus_tag="P5201_07865"
     CDS             complement(2141..>2589)
                     /gene="ybeY"
                     /locus_tag="P5201_07865"
                     /inference="COORDINATES: protein motif:HMM:NF014216.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004540 - ribonuclease activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="rRNA maturation RNase YbeY"
                     /protein_id="HRV98488.1"
                     /translation="PVFTRALCDLFPEGTLPAKVEISLSFLSLGEMKAQNRKYRGLDE
                     PTDVLSFPLWEEDGVFRPGRSLPTLPLGDILICPEYVRAAVSSEEEFQREMALMLAHG
                     FLHLLGWDHDTDERRSAMEVRQELIRKELLAFPEGAASGTAEEELR"
BASE COUNT          556 a          751 c          761 g          521 t
ORIGIN      
        1 gaagcacggg ggtgcgtacc ttcccgagga cggagaggat ttcttcgtcc tcgtgggcga
       61 ccttatctgt tacgtcgtcg aggcggagga cagaaccgtc gcccccgagg acgtaacaga
      121 taaggtcggc ctcctccagc gattctacgg ctgcatccac cagcgttctg ccaagcttgt
      181 gcttcgggag atggatcccc ggagtgtcgg agaagatgat ttgaaagtct tccccgttgt
      241 agacgcaacg gatgacgttt ctcgttgtct gcggtttctc ggaaacgatg gaaaccttgt
      301 agtcaagaag cctgttgacc agcgaggact tcccgacgtt gggacggcct gcgaggacga
      361 cgttccccgc acggaagccg atccctgcga aggggttgtt catcgttccc cgctctgctc
      421 cgtcagaagg aacggggccg gaaagagctc tgccaatgtg gtcacgacga atgaatctcc
      481 ctgttcaaag acaagcagca tttccgggtt gaactccgcg aggaactgcc ggcacgcgcc
      541 gcacggcagg cacacttctc ccctttttcc gacaacggct gcggcaagag gctttttgcc
      601 tccgcgggca atcatcgcga cggcggcgct ccgctcggcg cagatggaga gtccgtagga
      661 gccgttctcc acgttgcacg caaggataag atcgtccata ccctctacga acagagccgc
      721 cccgacaggg aagcctgagt agggggagta tgatgcgcgc tgcgcctcac gcgccgcgtc
      781 aaggagcttt tgaggcgacg gccatgatgc aggccaggaa acgggcgacg taaacagggt
      841 cattcgacaa cctccggtgg gataaaatac ccccgctcct tcggaaggag acggaggagc
      901 ttaattcgat ggtcttccac ttcaagaact tcaatctccc agttgcctgc gccgtccggc
      961 gagcggtagt gtatcttctc gcccggggag ggaaaatctc ccgatatgga gagcaccagt
     1021 cctccgagac tttcggcgtc ctcggattcg aacgacgctt ccagaaggtc gctgagatcc
     1081 tccaggctga cgtgcccctg aacgaggtaa ctgccgtcct cttcgggggt aaaggaaggt
     1141 ttttccttgt cgtattcgtc ctgtatctcc ccgacgatct cctcaaggag gtcttcaagg
     1201 gtggcgatgc ctgccacgcc gccgtactcg tcgacgacga tggccatgtg gacgtgtttc
     1261 cccttcatgt tgttgaagag ttcgacgacg cgcatgctct caggcacgaa gagcgcgttc
     1321 ctcatgatgg aggagacggg ggtttcgagc tccccggcca ggaggttcgg aatagtgtcc
     1381 ttgacgtaga gaatgccgac gatgtcgtcg aggttctctt cgaaaacggg gacgcgggag
     1441 tgtccgtgct cctgaaagac cttcatggcc tccgcgacgg tgcagtttga aggcacggcg
     1501 tccatgtcgg tgcgcggcac catgatttcg tagaccctgg tctcctcgaa cgagatgatg
     1561 ccgtgtatca tccggcgctc cacctcttcg agcgcgccgg attcctcccc tatgttgacc
     1621 atctgctcga attcctctct cgttacgaag gggtgccggg ctttcaggtc caccttgaag
     1681 agaagaccga gcagccggac cgccgcgatc atgagccata cgaagggggc gaggaggata
     1741 tccagcagcc tcaaaaacgg gagcgaaaaa acgagcacga aatccgattg tattatagcg
     1801 acactcttcg gaaacacctc gccgaagatc actatgagcg ccgtcatcag cggaactgcc
     1861 agcaggacgc ctccctgacc tatcgtggca atcgccagag ttgttccgac cgcgcttgcg
     1921 gctatgttga cgaggttgtt cgaaatcaag acgatggtga gcgcacgctg cacatccttg
     1981 aggagccact cgaaaaaacg cttctgatac ggataccgtt ctatcagcgc aaggattttt
     2041 cctctgccag aggatgtgat cgctgtttca gaggcgctga aaaaggcaga taggaagagc
     2101 agtaccagca aaagcgccgc cgactgcgct acaagagaac tcaccggagc tcctcctccg
     2161 cggtcccgga agccgcacct tcgggaaagg ccagcagttc tttccgtatc agctcctgcc
     2221 gtacttccat agcgcttctc cgctcatcgg tatcgtgatc ccacccgagc aggtggagga
     2281 aaccgtgagc aagcatcagc gccatttctc tctggaattc ctcttccgaa gagacggcgg
     2341 cgcgaacgta ctccgggcat atcagaatat cgcccaaagg aagcgtcggg agcgaccgcc
     2401 ccggacggaa gactccatcc tcttcccaaa gggggaagga gaggacgtcc gtcggttcgt
     2461 ccagtccgcg gtatttccgg ttttgtgcct tcatttcgcc gagagagaga aaggacaggg
     2521 aaatctcgac cttcgcgggg agagtcccct ccggaaaaag atcgcaaagg gcgcgcgtaa
     2581 ataccggct
//
LOCUS       DAOTIL010000350         2586 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminobacteriaceae bacterium isolate AMR_MDS_3026
            NODE_47396_length_2586_cov_4.170288, whole genome shotgun sequence.
ACCESSION   DAOTIL010000350 DAOTIL010000000
VERSION     DAOTIL010000350.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868050
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminobacteriaceae bacterium (wastewater metagenome)
  ORGANISM  Aminobacteriaceae bacterium
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae.
REFERENCE   1  (bases 1 to 2586)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2586)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:19:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,224
            CDSs (total)                      :: 3,198
            Genes (coding)                    :: 3,184
            CDSs (with protein)               :: 3,184
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2586
                     /organism="Aminobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_47396_length_2586_cov_4.170288"
                     /isolate="AMR_MDS_3026"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:3034609"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(292..642)
                     /locus_tag="P5201_07870"
     CDS             complement(292..642)
                     /locus_tag="P5201_07870"
                     /inference="COORDINATES: protein motif:HMM:NF025120.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HRV98489.1"
                     /translation="MTGCEYKPVPHNHDEFLAAMSEREGFTSAYAALEDEYRFVRELL
                     AARIRAGLTQEEVAESMGTSKSAVSRLEAGGKHSPSVQTLRKYAKAVGCDVEIRLVEK
                     PSRCASPAKNSSAM"
     gene            complement(629..961)
                     /locus_tag="P5201_07875"
     CDS             complement(629..961)
                     /locus_tag="P5201_07875"
                     /inference="COORDINATES: protein motif:HMM:NF017757.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HRV98490.1"
                     /translation="MAYSVEYFNDRVRAAIEAWPVGILADYARILEMLMEFGPEVGMP
                     YSRPMGDGLFELRPRGREGIGRALYCYMRGRRLLILHAFVKKTGSTPQKDLNRARERL
                     KEVRHDWM"
     gene            complement(1025..1675)
                     /locus_tag="P5201_07880"
     CDS             complement(1025..1675)
                     /locus_tag="P5201_07880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014163599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HRV98491.1"
                     /translation="MKIRVLMIDDHTLFLAGLEDILAREDDIEVVGVAGSCREGVSLA
                     MEKRPDVIVLDLAMPGTSGVEAARILAEALPGVRMAALTMHLDRQMVFEALKSGMTGY
                     ILKEATPEEFLFALRVVARGETWLSPKVAAIVANDFVRGAAQEAPSLPVLSDREREVL
                     HLLVKGAGTREIASRLHISKSTVDTHRRNILDKLGCENVTCLTRYALRHGLVDLDE"
     gene            complement(1672..>2586)
                     /locus_tag="P5201_07885"
     CDS             complement(1672..>2586)
                     /locus_tag="P5201_07885"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HRV98492.1"
                     /translation="DILGRPFREIWSEREREWQDIILQVATTGIYARYEGPSYDTGKY
                     LHAIAFSPTRSVVAVIFLDMTDWKRAEEALQEKEVLLTEYRQELRTLAARLSLAEEHT
                     RREISVKLHDRVGYSLVSLLTRLRTLAEKHDPDDIAPAIEEVEGLLSDTRSLTLEISS
                     PLLYEVGLEPALASLAERMLAPHGIAVDFRECGPQSHISMEIRILMYQMAQELLLNVI
                     KHAGARNVILRVQRGNKRVRMLVEDDGAGFSSKAPKHWGKWGGMGLFSIREKLHSVGG
                     EIRVFSEKGKGSTVALVAPLMFEGGKTS"
BASE COUNT          496 a          816 c          765 g          509 t
ORIGIN      
        1 gaactcaacg agtcggaccc gcgcctgaag gccggcgcga cgattcacag tttcgtgcag
       61 ggcaaaatga aagtagtcaa gctggagctc tgaaacgcat ccatcgtggg aggacgggag
      121 ggagcctctt cacgcaggca cgcatcctct atccgacgga cgaggggttc cgagtacatc
      181 cttgaagtga aagacggacg gaaagcggct tttttcggta gtattcttgc ctcgaagcat
      241 tcccgagact ccatgcgccg ctctccgtct ccgccgaaag cctcaaaaac ctcacatcgc
      301 cgaagagttt tttgcaggcg aagcgcaacg agacggtttt tcaaccagac gaatttcgac
      361 gtcgcaccct actgcttttg catacttcct cagcgtctgg acggaaggag aatgctttcc
      421 ccccgcttcc aggcgggaaa ctgcgctttt acttgttccc atggactccg caacctcttc
      481 ttgtgtgaga cctgcgcgta tcctggccgc caaaagttcg cggacaaatc ggtattcatc
      541 ctccagcgcg gcatacgcgg aggtaaaacc ttctctttcc gacatcgcgg ccagaaactc
      601 atcgtgatta tgaggaaccg gtttatattc acatccagtc atgacgcacc tccttcaatc
      661 tttcgcgggc tctattcaaa tccttctgcg gagtggagcc cgttttcttt acaaaggcgt
      721 ggagtatgag cagacgcctt cctctcatat agcagtacaa cgcacgtccg atcccttcgc
      781 gtcctcgggg gcgcagttcg aaaagaccgt ctcccatcgg acgggaatac ggcatcccca
      841 cttccggacc gaactccata agcatctcaa gaatccgcgc atagtcggca agaataccga
      901 cgggccacgc ttctatcgct gcacggactc gatcattgaa gtattcgacg gaataagcca
      961 tgtccgcatg ttatcaaata tgagaacaca cgtcaatgca gagtgactta aaaaaacttg
     1021 cccctcactc gtccaggtcg accaggccgt ggcgcagggc gtagcgggtg aggcaggtga
     1081 cgttttcgca gcccagctta tcgaggatgt tgcgccggtg ggtgtccacg gtgctcttgc
     1141 tgatgtgcag acgagacgct atctcacgcg ttcccgcccc tttgacgagg agatggagta
     1201 cttcgcgctc gcgatccgag aggacaggaa ggctcggcgc ttcctgagcg gcgccgcgga
     1261 cgaagtcgtt cgccacgatg gcggccacct tcgggctgag ccaggtctcc ccgcgcgcca
     1321 ccacgcggag cgcgaagagg aactcctccg gagtggcctc tttcaaaata tagccggtca
     1381 tgccgctttt gagcgcctcg aagaccatct ggcggtcgag gtgcatcgtg agcgccgcca
     1441 tgcggacgcc cggcagcgcc tcggcgagga tgcgcgccgc ctccacgccg ctggtcccag
     1501 gcatcgcgag gtcgaggacg atcacgtcgg ggcgcttctc catcgccagc gacacgcctt
     1561 cacggcagct cccggcgacg ccgaccactt cgatgtcgtc ctcgcgggcg agaatgtcct
     1621 ccaacccggc caggaagagc gtatggtcgt cgatcatcag cacgcggatc ttcatgacgt
     1681 ctttcctccc tcgaacatga gcggcgcgac gagcgcgacg gtgctccctt tccccttctc
     1741 cgagaagacg cgaatctctc cgccgacgga gtgcagtttt tcccggatgc tgaaaaggcc
     1801 catgccgccc cacttccccc agtgcttcgg cgccttggag gagaaccccg cgccgtcgtc
     1861 ctcgacgagc atgcggacgc gcttgttccc ccgctgcacg cgaaggatca cgttccttgc
     1921 cccggcgtgc ttgatcacgt tgagcagcag ctcttgcgcc atctggtaca tcagaatgcg
     1981 gatttccatc gagatgtgcg attgcgggcc gcactcgcgg aagtcgacgg cgatgccgtg
     2041 cggggcgagc atccgttcgg cgagcgaggc gagcgccggt tccaggccga cttcatagag
     2101 cagcggcgag ctgatctcca gcgtcaggga gcgcgtgtcg gagagcagcc cctccacctc
     2161 ctcgatggcc ggggcgatgt cgtcggggtc gtgcttttcg gcgagggtac gcagacgcgt
     2221 gagcagcgag acgagcgagt accccacccg gtcgtgcagc ttgacggaga tctccctgcg
     2281 ggtatgctcc tccgcgaggg agagacgcgc ggcgagggtg cgcagctcct gccggtactc
     2341 cgtaaggaga acctccttct cctgaagcgc ctcctcggcc cgcttccagt cggtcatgtc
     2401 gaggaagatc accgcgacga ccgagcgcgt ggggctgaag gcgatggcgt ggaggtactt
     2461 ccccgtgtcg tagctgggtc cttcgtaccg ggcgtagatc cctgtggtgg ccacctggag
     2521 gatgatgtcc tgccactccc gctccctctc cgaccatatc tcgcggaagg ggcggccgag
     2581 gatgtc
//
LOCUS       DAOTIL010000351         2579 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminobacteriaceae bacterium isolate AMR_MDS_3026
            NODE_47570_length_2579_cov_3.317353, whole genome shotgun sequence.
ACCESSION   DAOTIL010000351 DAOTIL010000000
VERSION     DAOTIL010000351.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868050
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminobacteriaceae bacterium (wastewater metagenome)
  ORGANISM  Aminobacteriaceae bacterium
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae.
REFERENCE   1  (bases 1 to 2579)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2579)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:19:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,224
            CDSs (total)                      :: 3,198
            Genes (coding)                    :: 3,184
            CDSs (with protein)               :: 3,184
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2579
                     /organism="Aminobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_47570_length_2579_cov_3.317353"
                     /isolate="AMR_MDS_3026"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:3034609"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..717)
                     /locus_tag="P5201_07890"
     CDS             complement(<1..717)
                     /locus_tag="P5201_07890"
                     /EC_number="1.1.1.1"
                     /inference="COORDINATES: protein motif:HMM:NF012678.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase"
                     /protein_id="HRV98493.1"
                     /translation="MRGFALRKFVAPEILFGRGALSLAGQYARNLGGGRVFVATDPGV
                     IEAGLLEPVLESLEQEDLDYTVFSDIRPNPTASMVMAGARVFADERCDLILALGGGSS
                     MDCAKAVGAVHANASHVSEFVGVDRIPLPCPPVICIPTTAGSAADVSQFAIIKNEETK
                     VKNAIVSKSIVPDVALVDPETTLTMDRNLTAATGLDALTHAVEAYVSRASSDLTDLHA
                     LQAIRLVGEFLPKVLDNLSDI"
     gene            complement(810..1823)
                     /locus_tag="P5201_07895"
     CDS             complement(810..1823)
                     /locus_tag="P5201_07895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005659150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketoacyl-ACP synthase III"
                     /protein_id="HRV98494.1"
                     /translation="MKGFGAGVRAISYYLPPKVVDNKALVEEFGTWTEEKIYQKTGIR
                     ERHIVDGELVSDLATRAGEKFFEEHGIGRDEIDFLLLCTESPDYYLPATACVVQDRLG
                     LKKTTGALDFNLGCSGFVYGLALAKGLIGTGIAKKVLLITADTLSRTVHPMDKSTRTI
                     FGDAAAATLIEGSDTARIGDFVLGTDGSGMDKLIIPAGAWAAPRSPETAVERTNKWGN
                     TRSAENMYMNGPEVLKFTVETVPPAVQQALDVHSLKLEDIDLFVFHQATKLILEHLRK
                     EIGIPEEKFYTNIENKGNTVSATIPMALRDAADEGRLRPGDRVMVVGFGVGFSWGATI
                     LRW"
     gene            2091..>2579
                     /locus_tag="P5201_07900"
     CDS             2091..>2579
                     /locus_tag="P5201_07900"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012376.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair exonuclease"
                     /protein_id="HRV98495.1"
                     /translation="MGSGTLKFIHCADLHLDSPFSGLAETSPALGSFLRAASFSAFRN
                     AVDCALRNEADFVLISGDVYDGEDRSVRAQLFFLEQLKRLSDAGIASFTVHGNHDPLS
                     GWELDRDLPPLAYRFGSSEVESVPLTVKGERVGTVHGCSYPVRDVRENLARGFAGRRT
                     EGV"
BASE COUNT          471 a          811 c          775 g          522 t
ORIGIN      
        1 gatgtcggag aggttgtcca aaactttggg aaggaactcg cccacgaggc gaatggcctg
       61 gagagcgtgc aggtcggtaa gatcggacga tgcccgcgag acgtaggcct cgactgcgtg
      121 cgtcagcgcg tccagtccgg tggccgcagt gaggttccgg tccattgtga gcgtggtttc
      181 ggggtcgacg agcgcaacgt ccgggacgat ggatttgctg acgatggcgt tcttcacctt
      241 cgtctcttcg ttcttgatga tggcgaactg ggagacgtcg gccgcgcttc cggcggtcgt
      301 cgggatgcag atcaccggag gacaggggag cggtatgcgg tcgacgccga cgaactcgct
      361 cacgtgggag gcgttcgcgt ggaccgcgcc caccgctttt gcacagtcca tggagcttcc
      421 gcctcccagg gccaggatca ggtcgcaccg ctcatccgcg aagacgcgcg cccccgccat
      481 gaccatcgag gcagtgggat tcggccgaat atcggagaac acagtgtagt caaggtcctc
      541 ctgttcgaga ctctcaagga cgggttcaag caagcccgct tcgatgactc cgggatccgt
      601 ggcgacgaaa accctgccgc cgccgagatt acgggcgtac tgacccgcga gggagagggc
      661 ccctctcccg aagagtattt caggggcgac gaactttctc agggcaaaac cgcgcatact
      721 ctcatcccct tttgtcacaa taaaggcggg ggtgtattcc cccgccttta ttttatcaca
      781 aaaatggaat aatgttgcgg tactctccct taccagcgga gaatcgtcgc tccccaggag
      841 aagccgacgc cgaagccgac gaccatgact ctgtcgccgg ggcgcaatct tccctcgtcg
      901 gccgcgtcgc gcagggccat cggaatggtc gcgctcacgg tgttcccctt gttctcgatg
      961 ttggtgtaga acttctcctc cggaatgccg atctccttgc ggaggtgctc cagaatcagc
     1021 ttcgtcgcct gatggaaaac gaagaggtcg atgtcctcca gcttcagcga gtgcacgtcc
     1081 agagcctgct gtactgcggg cggaacggtc tccacggtga acttcagcac ctcggggccg
     1141 ttcatgtaca tgttctccgc gctccgcgta ttgccccact tgttcgtccg ttcaacggcc
     1201 gtctccggtg atcgcggggc cgcccacgcc ccggccggaa tgatcagctt gtccatcccc
     1261 gagccgtccg ttcccagcac aaaatcccct atgcgcgcag tgtccgaacc ttcgataagc
     1321 gttgccgcgg cggcgtcgcc gaagatcgtg cgcgtgctct tgtccatcgg atggacggtg
     1381 cgagacagcg tgtccgccgt gatcagcagc accttcttcg cgatccccgt ccctatcagt
     1441 cccttcgcga gggcgagccc gtagacgaac cccgagcagc ccaggttgaa gtcgagcgcc
     1501 cccgtcgtct ttttcagccc cagtctgtcc tgcaccacgc aagcggttgc cgggaggtag
     1561 tagtcggggc tctcggtgca cagaagcaga aagtctattt cgtctcttcc gatgccgtgc
     1621 tcctcgaaaa acttctcacc ggcgcgcgtc gcaagatcgg agacgagctc cccgtccaca
     1681 atatggcgct cccgaatacc cgtcttctgg tagatcttct cctcggtcca tgtgccgaac
     1741 tcctccacca gcgccttgtt gtccaccacc ttcggcggaa ggtagtatga aatagcccgt
     1801 actccggctc caaatccttt catctctcga acgcctccat aactagatgt tccttattta
     1861 cagaatgtgc atcatactcg acgcggtgct attatcccat tgtcaccgat aagtgacaag
     1921 tcttccgttg tcctgaaaat gcgcgctgaa tgctcttgca aagaaacggc gggtggaata
     1981 aagtaaggac acctttcgcg gcccggaaaa ccgctccgcg aagaagaccg gctccgggag
     2041 gacgctccga cgcgtttccc ggtgccccga atgaaaggag tggcgctctc atgggcagcg
     2101 gcacgctgaa attcatacat tgtgcggatc ttcatctcga cagccccttc tccgggcttg
     2161 cggaaacctc ccccgcgctg gggagcttcc ttcgcgcggc gtccttctcc gccttccgga
     2221 acgccgtcga ctgcgcgctg cggaacgagg cggactttgt cctgatctcc ggagatgtgt
     2281 acgacggcga ggacagaagc gtacgtgcgc agctcttctt cctcgaacag ctgaaacggc
     2341 tttccgacgc ggggatcgcg tcgttcacag tgcacggcaa ccacgatccg ctctccgggt
     2401 gggagctgga ccgcgacctg cctcccttgg cgtaccggtt cggctcgtcg gaggtcgagt
     2461 ccgtgccgct gacggtgaag ggcgagcggg tgggaacggt gcacgggtgc agctaccccg
     2521 tgcgcgacgt cagggagaac ctggcgcgcg gcttcgcggg acggcgaacg gagggcgtg
//
LOCUS       DAOTIL010000352         2570 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminobacteriaceae bacterium isolate AMR_MDS_3026
            NODE_47761_length_2570_cov_2.795626, whole genome shotgun sequence.
ACCESSION   DAOTIL010000352 DAOTIL010000000
VERSION     DAOTIL010000352.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868050
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminobacteriaceae bacterium (wastewater metagenome)
  ORGANISM  Aminobacteriaceae bacterium
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae.
REFERENCE   1  (bases 1 to 2570)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2570)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:19:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,224
            CDSs (total)                      :: 3,198
            Genes (coding)                    :: 3,184
            CDSs (with protein)               :: 3,184
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2570
                     /organism="Aminobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_47761_length_2570_cov_2.795626"
                     /isolate="AMR_MDS_3026"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:3034609"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..667)
                     /locus_tag="P5201_07905"
     CDS             complement(<1..667)
                     /locus_tag="P5201_07905"
                     /inference="COORDINATES: protein motif:HMM:NF024118.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HRV98496.1"
                     /translation="MKITIHRGSREIGGVCIQVQSGDRSILLDAGSPLGESLSTVDLN
                     ALDFSDIVISHPHQDHFGLIETLSSKKTVYMGPIARKLIEATRVFLQQPPLICSFGEL
                     QSREWVDFGSDIRIMPYLMDHSCPDAFGLLIEAEGKRVYYSGDFRASGPRKEVFDYFL
                     ADPPRDVDVLLMEGTMMGRGNARFPNEKAVENGMIEVLKNLGDRACFLISSGRHVDRL
                     CAAY"
     gene            1159..1395
                     /locus_tag="P5201_07910"
     CDS             1159..1395
                     /locus_tag="P5201_07910"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRV98497.1"
                     /translation="MDKAVLRRLARIPEPDRGRLTAAIVDLRSDPEAKDILPLTGRTG
                     YRMRVGRWRVLLDIDFKSEKIFVYDLGPRGDVYK"
     gene            complement(1473..2021)
                     /locus_tag="P5201_07915"
     CDS             complement(1473..2021)
                     /locus_tag="P5201_07915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012868841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BMC domain-containing protein"
                     /protein_id="HRV98498.1"
                     /translation="MSKAIGLVEYKTIPKGIEAADAMLKASEVQLLFSSPICPGKYVT
                     IVGGEVDSVKTAVDKAVAVGDIFTVDSHVLTSIHPSVLPAITATVEIKKVEALGVVET
                     ISAVAAIQAGDVAAKAGNVQLVEIRIARGLGGKGFVLFTGDLGSVETSVRACEKKLGE
                     EGGIVSSAVIPSPHKDLISSVL"
     gene            complement(2018..>2570)
                     /locus_tag="P5201_07920"
     CDS             complement(2018..>2570)
                     /locus_tag="P5201_07920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006584208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HRV98499.1"
                     /translation="LGHKLRPDKLMRLAAYNSTCEHDAAATEAFLCCECGLCETACIM
                     GLQPWKLNKELKVRMNAAGIKNPHHERPERVNPFREYRLFPVTKLTYRLGLSKYIGRK
                     APLREEFSSPVKKVVLKLKQHIGAPAEPIVAPGDAVERGRMVARGEGPVSAHVHSSIS
                     GVVRAVEKDRIIVEATTDGRENE"
BASE COUNT          523 a          745 c          775 g          527 t
ORIGIN      
        1 tataagcggc gcagaggcgg tcgacatgtc tgccggaaga gatcaggaag caggcgcggt
       61 cgccgagatt tttcagcacc tcgatcattc cgttctcgac ggctttctcg ttcggaaacc
      121 gggcgttgcc gcgccccatc atcgtccctt ccatgagcag cacatccacg tctcgtggag
      181 gatcagcgag aaaatagtcg aacacttcct ttcgcggacc gcttgcgcga aagtcgccgc
      241 tgtagtagac gcgcttccct tcggcctcga tcagaaggcc gaaagcatcg ggacaggagt
      301 ggtccatcag gtagggcatg attctgatgt ccgatccgaa atcgacccac tcccggctct
      361 gtagttcgcc gaaggagcag atcaaaggag gctgctgcag aaatacgcgc gtcgcctcga
      421 tgagcttccg cgctatcggc cccatataca ccgtcttttt ggaggagagc gtttcgatca
      481 ggccgaagtg gtcctgatga gggtggctga tgacgatatc gctgaagtcg agcgcgttca
      541 gatcgacggt ggaaagagac tccccgagag gggaaccggc atcgagaaga atggaacgat
      601 caccggactg aacctgtatg cagacgccgc cgatctcccg gctccctctg tgaatggtga
      661 ttttcatgtc gcatccccct tgcaaaaaga tatgagacgc atgtcctgca ttcgaaccga
      721 ggaagtgttg cttttgatat catagcatgg aggggtgttc gattcagggc ttttagagag
      781 tgatctacaa gaaagaaatt accatagagt atgcagattt acctgatcgt ttctcgcaac
      841 gagcgcttac acgtgcggaa ccgccggcga agaatctttt tatgatatac ttttcaaaga
      901 ggtatccgca tgaatgcggg ctaaaaatcc ctcaagaaga atatctcgaa atggaaaggg
      961 gaggacgaaa atgccggctg tgaaggaaaa aaagaatgtc cctttgtggg cacgggagcc
     1021 cgacccgtta gagccgcccc tgactccgga agaagaggaa gccttcgaaa cgggcaggaa
     1081 gaatgtgcaa cgaaaagact gtctgactct ggaagaattc atcgaacagg tctccttgtt
     1141 gtgagctggg aggtgactgt ggataaagca gtcctccgtc gcctcgctcg cattcccgaa
     1201 cccgatcgcg gacgtttgac ggcggcgatc gtcgatttgc ggagcgaccc ggaggcgaag
     1261 gacatactcc ctttaacggg gcgcaccggg tatcgtatgc gcgttggccg ttggagagtg
     1321 ctccttgata tagattttaa aagtgagaag atcttcgtgt atgacctcgg ccctcgcgga
     1381 gacgtgtaca agtaaacgca gtcccgcaca aacactgagc cccggcgcgc atctaagtgc
     1441 gcgccggggc tcagtgtttg tcgaaaactc ccttacagca ccgaggagat caggtccttg
     1501 tgcggggagg ggatgacggc ggagctgacg atgccgccct cttcgccgag cttcttctcg
     1561 caggcgcgca ccgatgtttc cacgcttccc aggtcgccgg tgaagaggac gaatcccttc
     1621 ccgcccaggc cgcgcgcgat gcggatttcc acgagctgga cgttgccggc cttcgccgcc
     1681 acgtctcccg cctgaatcgc ggcgactgcg gagatggtct cgaccacgcc gagcgcctcc
     1741 acctttttaa tctcgacggt ggccgtgatg gcgggaagaa ccgacgggtg gatgctggtg
     1801 aggacgtgcg agtccaccgt gaagatgtcg ccgaccgcga cggccttgtc cacggcggtt
     1861 tttacggagt ccacctctcc gccgacgatg gtgacgtact tgccggggca gatgggcgac
     1921 gagaagagca gctgcacctc cgaagccttc agcatggcgt ccgccgcctc gatacccttc
     1981 ggtatcgtct tgtattcaac gagtcctatg gctttgctca ttcattctcc ctcccgtcgg
     2041 tcgttgcctc gacgatgatt ctgtccttct cgaccgcgcg cacgacgccg gagatcgacg
     2101 agtgcacgtg cgccgaaacg ggcccctctc cccgcgcgac catccggccg cgctccacgg
     2161 cgtcgccggg ggccacgatg ggttccgcgg gcgcgccgat gtgctgtttc agcttcagca
     2221 cgactttttt gaccggagag gaaaactcct cccgcaaggg ggccttccgt ccgatgtact
     2281 tcgacagtcc cagacggtac gtcagcttcg tcaccgggaa gaggcggtac tccctgaacg
     2341 ggttgacccg ctcggggcgc tcgtgatgcg gattcttgat gccggccgcg ttcatgcgga
     2401 ccttcagctc cttgttcagc ttccagggct ggagccccat gatgcacgcc gtctcgcaga
     2461 ggccgcactc gcagcagagg aaggcctccg tcgccgccgc gtcgtgctcg caggtgctgt
     2521 tgtaggcggc caggcgcatc agcttgtccg ggcggagctt gtgccccagc
//
LOCUS       DAOTIL010000353         2566 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminobacteriaceae bacterium isolate AMR_MDS_3026
            NODE_47851_length_2566_cov_2.887694, whole genome shotgun sequence.
ACCESSION   DAOTIL010000353 DAOTIL010000000
VERSION     DAOTIL010000353.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868050
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminobacteriaceae bacterium (wastewater metagenome)
  ORGANISM  Aminobacteriaceae bacterium
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae.
REFERENCE   1  (bases 1 to 2566)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2566)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:19:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,224
            CDSs (total)                      :: 3,198
            Genes (coding)                    :: 3,184
            CDSs (with protein)               :: 3,184
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2566
                     /organism="Aminobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_47851_length_2566_cov_2.887694"
                     /isolate="AMR_MDS_3026"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:3034609"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            81..2216
                     /locus_tag="P5201_07925"
     CDS             81..2216
                     /locus_tag="P5201_07925"
                     /inference="COORDINATES: protein motif:HMM:TIGR02074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PBP1A family penicillin-binding protein"
                     /protein_id="HRV98500.1"
                     /translation="MNWKHVQETKTSRCIDFAGRFCSAAALLLVRLSVIPILLWLSWE
                     FAVHAALRFDIEGVPSIEELRSYRPMRTTMLYDRYGKPFARFFIEDRVELPLSGMSPW
                     IPKAVVAAEDARFPSHRGVSLAAIARAAIENQRAGSIRQGGSTITQQLARNMFLSRER
                     TIERKAREAVIAFRLESEFSKEEILEKYLNEIYFGRGAWGVETASQVYFGKHARDLSL
                     GEAALLAGVIPSPNRTNPGASPDAAEAGKRRVLARMFETGAISARERSEAEAPVALVP
                     EPGNLPSAHVRYPYYAMRVLNDTLLPEYGVNGAYGGGLRVYTMLDPDLQEAGERIAAR
                     SKHQLAIVGLDSETGGVRALVGGKDWHVSQFDRAVQAYRQPGSAFKPLVYATLFEKKG
                     WSGATKIQDAPISFGSGRNRWSPRNYDGKFRGSVTVERAIISSLNVPAVRALSAAGAK
                     VVAETVRSMGITTPYLPDNPTMALGSASLTPLEMAAAFSVFGNGGYRIVPTLVREIRD
                     ADGKVLFKAAEEKKRVLSEYTANGIRDILIKAVSSGTGRAARIDGFQVFGKTGTTNDF
                     RDAWFVGGIPGFCAAVYVGDDDRKPLGKGATGGKIAAPIWQDFMEYAADTLCAPAAFP
                     KNTEKTPPVPRERSAQETTDPVKESETASPAPPKETPAVAPPPAEEKRPRIVQPALPP
                     AARAAPDAMLIETETEKKMRELLQKYNIE"
     gene            complement(2238..>2566)
                     /locus_tag="P5201_07930"
     CDS             complement(2238..>2566)
                     /locus_tag="P5201_07930"
                     /inference="COORDINATES: protein motif:HMM:NF015962.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0050532 - 2-phosphosulfolactate
                     phosphatase activity [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="2-phosphosulfolactate phosphatase"
                     /protein_id="HRV98501.1"
                     /translation="ERIGILCAGLYGRPAMDDAACAGLLAEQLLGQGAVLDDGAKMAL
                     SVWQAGMRNLSALLGASVHGGRLLSLGFAPDMAYAAEIDASNSVLVLSEVKELPCIVP
                     GNTPAA"
BASE COUNT          485 a          800 c          877 g          404 t
ORIGIN      
        1 gtacagttgc agaacaaaat aaaaaaataa tcgggttcct ctccgtcggg ttcgcaaggg
       61 gaagaaacag gggagggtcc atgaactgga agcatgtgca agagaccaaa acaagcaggt
      121 gtatcgactt cgcggggagg ttctgctccg ccgccgctct tttgctggtc cgtctttccg
      181 tgatccccat tcttctgtgg ctctcctggg agttcgccgt ccacgccgcg ctccgcttcg
      241 acatcgaagg cgttccgtcc atcgaggagc tccgctcgta ccgtccgatg aggaccacaa
      301 tgctctacga caggtacggc aagccgttcg cgcgcttttt catcgaggac agagtggagc
      361 ttcccctctc cggaatgtcc ccgtggatac cgaaggccgt cgtcgcggcg gaggacgcgc
      421 gcttcccgtc gcaccgcggg gtgagcctgg cggcgatcgc gcgggccgcc atcgagaacc
      481 agcgcgccgg gagcatccgt cagggcggca gcacgattac gcagcagctg gcgcgcaaca
      541 tgtttctctc ccgcgagcgg acgatcgagc ggaaggcccg cgaggcggtc atcgcattcc
      601 ggcttgaaag cgagttttcg aaggaggaga tcctggagaa gtatctcaac gagatctact
      661 tcggccgcgg agcgtggggc gtggagacgg cgtcgcaagt gtacttcggg aaacatgcgc
      721 gcgatctctc cctcggcgag gcggcgcttc tggccggggt gatcccctcc cccaaccgta
      781 ccaatccggg ggcgtcgccc gacgctgcgg aagcgggaaa gaggcgcgtg ctggcgagga
      841 tgttcgaaac cggcgcgatc tccgcgcgcg agcgctccga ggcggaagcg ccggtcgcgc
      901 tcgttccgga gccgggaaac cttccttcgg cgcacgtgcg gtatccgtac tacgcgatgc
      961 gtgtgctcaa cgacacgctg ctgcccgagt acggggtaaa cggcgcgtac ggcggaggac
     1021 tgcgcgtcta cacgatgctc gaccccgatc tgcaggaggc gggcgaacgc attgccgcga
     1081 ggtcgaaaca ccagctcgcg atcgtggggc tggactcgga gaccggaggc gtcagggcgc
     1141 tcgtcggcgg caaggactgg catgtaagtc agttcgacag ggccgtccag gcgtatcggc
     1201 agcccggatc ggcattcaaa ccgcttgtgt acgcaacgct cttcgagaaa aaggggtgga
     1261 gcggggcgac gaagattcag gacgctccga tttcgttcgg ctcggggcgg aacagatggt
     1321 cgcccagaaa ctacgacggg aagttccggg ggagcgtgac ggtcgagcgg gcgattatca
     1381 gctcgctgaa cgtgcctgcg gtccgggcgt tgagcgccgc gggggcgaaa gttgtggcgg
     1441 aaaccgtccg cagcatgggc atcaccacgc cgtaccttcc ggacaatccg acgatggcgc
     1501 tggggagcgc ctcgctgacg ccgctcgaaa tggcggcggc cttctcggtc ttcgggaacg
     1561 gagggtacag aatcgtcccg acgcttgtcc gcgagatccg cgacgccgac ggaaaggtcc
     1621 tcttcaaagc ggcggaggaa aagaagcgcg tcctctccga atacaccgcg aacgggatca
     1681 gggacattct catcaaggcc gtttcgtccg gaacgggacg cgccgcgcgc atcgacggat
     1741 tccaggtctt cggcaagacg gggacgacga acgactttcg ggacgcatgg ttcgtcggcg
     1801 gcattccggg cttctgcgcc gccgtctacg tgggcgacga cgacaggaag ccgctcggaa
     1861 agggcgctac cggcggaaag atcgcggccc ctatctggca ggattttatg gagtacgccg
     1921 cggacacgct ctgcgccccg gccgcgttcc cgaagaacac ggaaaagacg cctcctgttc
     1981 cgcgggagcg cagtgcgcag gaaacgacgg accccgtcaa ggagtcagaa acggcgtctc
     2041 cggcccctcc gaaggagacg ccagcggtcg ccccgccccc ggcggaagag aagcggccgc
     2101 gaatcgtgca gcccgcgctt cccccggcgg ctcgcgcggc tcccgatgcg atgctcatcg
     2161 agacggagac ggagaagaag atgcgcgagc tgctccagaa atacaatatc gagtagaacg
     2221 cgccactcct cgacgcatca agcggcaggg gtgtttccgg ggacgatgca ggggagttcc
     2281 ttcacctcgc tcagtacgag cacggaattg gaggcgtcga tctcggcggc gtaggccatg
     2341 tccggggcga accccagcga gagcaggcgt ccgccgtgca ccgacgcccc gagcagcgcg
     2401 gagaggttgc gcattccggc ctgccatacg gagagcgcca tcttcgcgcc gtcgtccagc
     2461 accgcgccct gtccgaggag ctgttcggcg agcaaccccg cgcaggccgc gtcgtccatg
     2521 gccgggcgtc cgtagagccc tgcgcagagg atgccgatac gctctc
//
LOCUS       DAOTIL010000354         2565 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminobacteriaceae bacterium isolate AMR_MDS_3026
            NODE_47870_length_2565_cov_3.545418, whole genome shotgun sequence.
ACCESSION   DAOTIL010000354 DAOTIL010000000
VERSION     DAOTIL010000354.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868050
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminobacteriaceae bacterium (wastewater metagenome)
  ORGANISM  Aminobacteriaceae bacterium
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae.
REFERENCE   1  (bases 1 to 2565)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2565)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:19:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,224
            CDSs (total)                      :: 3,198
            Genes (coding)                    :: 3,184
            CDSs (with protein)               :: 3,184
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2565
                     /organism="Aminobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_47870_length_2565_cov_3.545418"
                     /isolate="AMR_MDS_3026"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:3034609"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..214
                     /locus_tag="P5201_07935"
     CDS             <1..214
                     /locus_tag="P5201_07935"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRV98502.1"
                     /translation="AVRRDVSEEARLFPDCSRVHLVSLQEMKFPIEEFTALYQLLSDL
                     SKNLFFRSFPAHSPHVRFSRKTAATN"
     gene            complement(592..1773)
                     /locus_tag="P5201_07940"
     CDS             complement(592..1773)
                     /locus_tag="P5201_07940"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HRV98503.1"
                     /translation="MENWDDSLLDQLEELNEIVRKAEEESAAAEIFTENGEHALCKAV
                     LWSMEQAVVVLSLLYDESGEAEDCICEKVNPAFERVSGISEERAVGSKGSEIFGEKIP
                     YFDIFVRVAETKSKVRFKAHYPKIGKYFDMWIFSPEWGRIALVFSDVTDSVLAEREQQ
                     LDRRFLDEILDSMNVWLMIFDKRWNPILWNKAAERLSGYCSEEALSPGSRVLEKLFPE
                     ELYREYVLEEMQKAAHRPEGVPGELETEVVVNGGRTRTIAWTCSRWKSEEENEEGLIA
                     AGRDLTEMRALEGALRESERRYSSIFLNSNMVMLIVDPEECRILDCNDAACRFYGYTR
                     EELTGMSSFDINMTPPEEFKRHLLRIRNGERSSFVFHHRRKDGALRKVDVQASPVLYD
                     G"
     gene            complement(1805..>2565)
                     /locus_tag="P5201_07945"
     CDS             complement(1805..>2565)
                     /locus_tag="P5201_07945"
                     /inference="COORDINATES: protein motif:HMM:NF015894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="FapA family protein"
                     /protein_id="HRV98504.1"
                     /translation="FSVKAGRDVSVSGSAFNARIEASGSCIVEGGIVGEHCEIRAGED
                     IRAGFVEYGLLISGVNIEILGYTLFATLEARQSVLVQGKNRRGIIGGVTTAGTTIKAL
                     SAGSAMEPRTLLEAGRDAVRNRNLAALTAKMAELEAMKTKIEQAMLSIKRPGLGFDLE
                     HLSEEEKGKLFLLARYHKKVGENMAELAGKIEDEKRAILAERKGTPLIKIRDKIYANV
                     TLKIWESQLTVSSAEFCAGYYFDRKNGSIQKGLY"
BASE COUNT          539 a          821 c          633 g          572 t
ORIGIN      
        1 ggcggttcgc cgagacgttt cagaagaagc aaggcttttt ccagactgct cacgcgtaca
       61 cctcgtttcc ttacaggaaa tgaagtttcc catagaagaa tttactgcac tatatcagct
      121 actcagtgat ttgtcaaaaa atcttttctt ccgctctttc ccggcgcact cgccccacgt
      181 acgcttcagc agaaaaactg ccgctacaaa ttaacagaga gcaaacgaac cgcctcggac
      241 acgcgtccga aacggttcgt taggcagttc tgaaagagaa aagggaaaac gggtacgaaa
      301 atgctaagaa caagaaagga ggacgctgta acgtctcatc aaagactgtt ggaaactctt
      361 cgatcaactc gccggcctcc gccggagagc ggatacaaac atctggactg tcgccgctgg
      421 caaaactgcc cggcggagag tttctttttc ggtatctcgg tcgaagaacg acgtcgttca
      481 gccggatctc aagagaggag gggaagcgcg ctccgtccgc taccgcgaga aaccatcttc
      541 atgcttctgt tcgccctcgg ccatctcccg tacaagagaa aaaacaagcg cttagccgtc
      601 gtagagcacc ggggaagcct gtacgtcaac cttccgcagc gccccgtcct tgcgccggtg
      661 gtggaagacg aagctgctcc gctcgccgtt gcgtatccgc aacaaatgcc gtttgaactc
      721 ctccggcggg gtcatgttga tatcgaacga gctcatgcct gtcagctcct ctctggtgta
      781 gccgtagaag cggcaggcgg cgtcgttgca gtccagaatt ctgcactcct ccggatcgac
      841 gatgagcatc accatgttcg agttgaggaa gatcgaggaa tagcggcgct cgctctccct
      901 gagggcgccc tccagcgcgc gcatctccgt cagatccctc ccggcggcga tgaggccttc
      961 ttcattctcc tcttcgctct tccaccggct gcacgtccat gctatcgtgc gggtccggcc
     1021 gccgttcacc acgacctcgg tctccagctc accgggaacc ccctccggcc gatgcgcggc
     1081 cttctgcatc tcttcaagca cgtactcacg gtacagctcc tccgggaaga gcttctccag
     1141 aacgcgggaa ccgggagaca gggcctcttc gctgcagtag ccgctcagcc gttctgccgc
     1201 cttgttccag aggatgggat tccagcgctt gtcgaagatc atcagccaga cgttcatgct
     1261 gtccagtatc tcgtcgagga aacgccggtc gagctgctgc tcccgctccg ccagcaccga
     1321 atccgtcacg tcgctgaaga cgagcgcgat cctcccccac tcgggggaga aaatccacat
     1381 gtcgaagtac ttgccgatct tcgggtagtg cgctttgaac cgtaccttgc ttttagtctc
     1441 ggcaacgcgg acaaagatgt cgaaatacgg gatcttctct ccgaatatct cgctcccctt
     1501 gctgcctacg gctcgttcct cggaaatacc ggaaacacgt tcgaaagcgg ggttgacctt
     1561 ttcacagatg caatcctccg cttcgccgga ttcatcgtac agaaggctaa gcacgacaac
     1621 cgcctgttcc atgctccaga ggaccgcttt acagagagcg tgttcgccgt tctctgtaaa
     1681 aatctcggcg gcggcgcttt cttcttcagc tttccggacg atctcgttca gctcttccaa
     1741 ctggtccaaa aggctgtcgt cccaattttc caccctatcc aacccctctc cgccgaaacc
     1801 cggctcagta cagccccttc tgtatgcttc cgttcttccg gtcgaaatag taacccgcgc
     1861 aaaattccgc cgaagagacc gtcagctgcg attcccatat cttcagcgta acgttggcgt
     1921 aaatcttgtc ccggattttt atgagcggcg ttccttttcg ttccgcgagt atcgcccgct
     1981 tttcatcctc aatcttgccc gcaagctcgg ccatattctc gccgaccttc ttatggtagc
     2041 gcgccaggag aaaaagcttc cccttctcct cctcggaaag atgctccagg tcgaagccga
     2101 gacccgggcg ctttatcgag agcatggcct gttcgatctt cgttttcatt gcttcaagct
     2161 ccgccatctt cgccgtcaat gctgctaaat ttctgtttct gaccgcatcg cgccccgctt
     2221 cgagcagagt cctcggttcc atggcggaac ctgcggagag cgccttgatc gtcgttcctg
     2281 cggtcgtaac gccgccgata atgccgcgcc ggttcttccc ctgcacaaga acggactgcc
     2341 tcgcttcgag cgtggcaaag agggtatagc ccaaaatctc gatgttcacc ccggaaataa
     2401 gcagaccgta ctcaacgaac cccgcacgta tatcctcgcc cgccctgatt tcacaatgtt
     2461 caccgacgat tccaccctcg acgatgcagc tccccgaagc ctcgatacgt gcgttgaagg
     2521 cggaacccga tacggaaaca tcgcgccccg ccttcacgga aaacc
//
LOCUS       DAOTIL010000355         2562 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminobacteriaceae bacterium isolate AMR_MDS_3026
            NODE_47957_length_2562_cov_3.728361, whole genome shotgun sequence.
ACCESSION   DAOTIL010000355 DAOTIL010000000
VERSION     DAOTIL010000355.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868050
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminobacteriaceae bacterium (wastewater metagenome)
  ORGANISM  Aminobacteriaceae bacterium
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae.
REFERENCE   1  (bases 1 to 2562)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2562)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:19:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,224
            CDSs (total)                      :: 3,198
            Genes (coding)                    :: 3,184
            CDSs (with protein)               :: 3,184
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2562
                     /organism="Aminobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_47957_length_2562_cov_3.728361"
                     /isolate="AMR_MDS_3026"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:3034609"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..325
                     /locus_tag="P5201_07950"
     CDS             <1..325
                     /locus_tag="P5201_07950"
                     /inference="COORDINATES: protein motif:HMM:NF019140.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRV98505.1"
                     /translation="ERIISWILSSDVDSMVKIQGIGRKTAERLCFEMRPHLTGEDAEI
                     PGDFFSSAGRKAETAMEALRSLGFTPADVGSVFRKLKESGALEGDDLTEEKILRLALK
                     ELKRN"
     gene            337..1392
                     /gene="ruvB"
                     /locus_tag="P5201_07955"
     CDS             337..1392
                     /gene="ruvB"
                     /locus_tag="P5201_07955"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005662067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0009378 - four-way junction helicase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration DNA helicase
                     RuvB"
                     /protein_id="HRV98506.1"
                     /translation="MTEEIKRFLDPNDEPGEDENSLRPLSLHDFLGQEKLKEKLSIFI
                     EAASKRGEALDHTLFYGPPGLGKTTLAGIIAREMGGQLRVTTGPSLEKTGDLAAILSN
                     LEPNDVLFIDEIHRLPSNVEEILYPAMEDYALHIIVGKGPLANNICLTLPRFTLVGAT
                     TRLGLLTSPLRARFGIVEQLSLYSEDELRSIVARGARVLGVAIHDDAALEIARRSRGT
                     PRIALRLLRRVRDVAEVRALTSVDSATAFSALNMLGLDDLGLDEGDRRILRVIVELFD
                     GGPVGLSTLGAALNEETQTIEDIYEPFLIQQGLLERTPRGRKATKNCWIYLGKTPPPE
                     GNEQWQLHLPVEEGAEG"
     gene            1521..>2562
                     /locus_tag="P5201_07960"
     CDS             1521..>2562
                     /locus_tag="P5201_07960"
                     /inference="COORDINATES: protein motif:HMM:TIGR02669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpoIID/LytB domain-containing protein"
                     /protein_id="HRV98507.1"
                     /translation="MLPQTAAARTVSVGLAIGQSSVSVSSSSALSVTDGSGKKHALGT
                     TAVFRSAGTNSVVAGNRTLRLPVSISSRHPLSFNKRRYRGGFRLVSSGSGITLLNVLD
                     LEEYLRGVLKMEVNPAWSMEALKAQAVISRTYALRSVLQNRGKGGYDLRDTVMSQVYR
                     GMNAEDPKTDQAIRATKGMVLKYGGEIAFTPFHSHSGGATADVTTVWGGNMPYLKGVN
                     EDFQTESPHSTWEASLSAAQVEDALRSAGVNVGRLQELRVEQSDPFGRPTTLRAIGTS
                     GSKTLKSHSFRMAAGSNVIRSTLFSISSAASSSSAPIPLASLSQPKPVPPSPPAGTGV
                     PTSLTPMSAAEER"
BASE COUNT          561 a          654 c          744 g          603 t
ORIGIN      
        1 agaacgtatc atttcgtgga ttctctcgtc tgacgttgac tccatggtca agattcaggg
       61 gataggaaga aagacagccg agcgtctttg tttcgagatg cgtcctcatc ttaccggaga
      121 ggatgcggag atcccggggg actttttttc ttccgcaggt agaaaagcag agaccgctat
      181 ggaggcgctt cgttcacttg ggtttacgcc ggcggatgtg ggatctgttt ttcggaagct
      241 caaggagtcc ggggctcttg aaggggacga tcttaccgaa gagaaaattt tgcgccttgc
      301 cctgaaagaa ttaaagcgca actgaggtga taatcagtga cagaagaaat aaagcgcttt
      361 ctggatccaa acgatgaacc cggagaggat gaaaactctc ttcgtcctct ctctttacac
      421 gacttcctcg gacaggaaaa actgaaggaa aaactctcaa tttttatcga agccgcctcc
      481 aaacgggggg aggccttgga tcacactctt ttttacggtc cgcccggtct tggtaagacc
      541 acccttgcag gtatcattgc cagggaaatg ggaggccagc tgcgtgtgac aacgggacct
      601 tcgctggaaa aaaccggaga tcttgcggca attttgtcga atcttgagcc gaacgacgtt
      661 cttttcattg acgagattca caggctccct tcaaacgtgg aagagatcct gtaccccgca
      721 atggaggact acgcgttgca cattatcgtg ggaaaggggc ctttggcgaa caacatatgt
      781 ctgacgctcc cgaggtttac ccttgtcggc gcgacgactc ggctcgggtt gctcacctcg
      841 cctcttcgtg cgcgtttcgg aatagttgag cagctctctc tctattcgga agatgaactc
      901 aggagtattg tcgcgcgtgg cgccagggtg ctcggtgtag cgatacatga cgacgcggcg
      961 cttgagatcg ccaggcgttc gcgcggcact ccgcgtatcg cgcttcgtct tcttcgcagg
     1021 gtccgcgacg tcgcggaggt ccgcgccctt acatctgttg acagcgcaac ggctttttct
     1081 gctcttaata tgctcgggtt ggatgatctg gggcttgatg agggtgatag aagaattctc
     1141 agagttatcg tcgaactgtt cgacggcgga cctgtcggcc tttcgacgct tggcgccgcg
     1201 ctcaacgagg agacccagac gatcgaagat atctacgaac cttttttgat ccagcagggg
     1261 cttcttgagc gaacgccccg gggacggaaa gcgacgaaaa attgttggat atatctcggg
     1321 aaaacacctc cgcccgaggg gaacgagcag tggcagttgc atcttcctgt tgaggagggg
     1381 gctgaaggat gaattctccg tgaaaaatag tagtcctgga ttgatgaaat aagaaaaatg
     1441 ggggtaagcc aatgttctta ctgagaaaaa agtgtttctt ccgccgagct cttcttctag
     1501 tctcaattct gagtattgtt atgctgccgc agacggcggc agccagaact gtttctgtcg
     1561 gtctcgctat cggccagtca tctgtttcgg tttcctcctc ttctgctctt tccgtgacgg
     1621 atgggagcgg gaaaaaacat gcgctgggta caacagcagt attccgtagt gcgggaacga
     1681 acagtgttgt cgcgggaaac cgtacgttac gtctgcctgt gagtatctcc tcgcgccacc
     1741 cgctttcttt caataaaaga cggtatcgag gcggattccg cctggtatct tcaggctcgg
     1801 ggattacgct tctgaacgtc ctcgatcttg aggagtacct tcggggagtg ttgaagatgg
     1861 aggtgaatcc tgcctggtcc atggaagcgc tcaaggcgca ggcagtcatc tcaagaacgt
     1921 atgctcttcg aagcgtcctt cagaacaggg gcaagggggg gtacgatctt cgcgataccg
     1981 ttatgtcgca ggtctaccgc ggcatgaacg ctgaagaccc caagaccgac caggctatac
     2041 gcgcgacaaa gggaatggtg ttgaaatacg gcggggagat cgcattcacc cctttccatt
     2101 cgcatagcgg gggagcgact gccgatgtga cgacggtgtg gggcggaaac atgccgtatc
     2161 tcaagggtgt caatgaagat tttcagacgg agtctccgca ttcaacatgg gaagccagtc
     2221 tttcagcggc ccaggttgag gacgctcttc gttctgcagg agtgaacgta gggcgtcttc
     2281 aggagcttcg ggtggagcag tcggatccgt tcggcaggcc aacaacgctt cgcgccatcg
     2341 ggacgagcgg gagcaagacg ctcaagagtc acagcttccg tatggccgca gggagcaatg
     2401 tgatcaggag tacgctcttt tccatctcgt ccgcagcatc ctcctcttcg gctcctattc
     2461 cccttgcttc gctttctcag ccgaagcctg ttcccccttc gcctcccgca ggaacgggcg
     2521 tccccacgtc gctgacgccg atgagcgcgg cggaagaacg ac
//
LOCUS       DAOTIL010000356         2557 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminobacteriaceae bacterium isolate AMR_MDS_3026
            NODE_48083_length_2557_cov_3.350120, whole genome shotgun sequence.
ACCESSION   DAOTIL010000356 DAOTIL010000000
VERSION     DAOTIL010000356.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868050
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminobacteriaceae bacterium (wastewater metagenome)
  ORGANISM  Aminobacteriaceae bacterium
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae.
REFERENCE   1  (bases 1 to 2557)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2557)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:19:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,224
            CDSs (total)                      :: 3,198
            Genes (coding)                    :: 3,184
            CDSs (with protein)               :: 3,184
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2557
                     /organism="Aminobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_48083_length_2557_cov_3.350120"
                     /isolate="AMR_MDS_3026"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:3034609"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..618)
                     /locus_tag="P5201_07965"
     CDS             complement(<1..618)
                     /locus_tag="P5201_07965"
                     /inference="COORDINATES: protein motif:HMM:NF025696.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix domain-containing
                     protein"
                     /protein_id="HRV98508.1"
                     /translation="MAKRRSEFVQWFGPVLDALRKLGGSGTPQEVSNCVAQTLNIPES
                     KLNETIKSGASKFHNQVCWARQYLVWEGYIDGSLRGVWKLTKIGEEKRIDLEEARAIF
                     LKWVDINAQKRKKKNGVPQIQENDLDDTDGEENEIDDDSSINVDYRQRVLSYLRSMTP
                     KNFERFCLLLLRVNKFENLELTGGSHDEGIDGKAILRINPFVSFKV"
     gene            complement(625..771)
                     /locus_tag="P5201_07970"
     CDS             complement(625..771)
                     /locus_tag="P5201_07970"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRV98509.1"
                     /translation="MRLEDLHPETVVRGILPDALVTVAAVEWHGSEAHPPLSRSPLSL
                     KARK"
     gene            complement(1159..1410)
                     /locus_tag="P5201_07975"
     CDS             complement(1159..1410)
                     /locus_tag="P5201_07975"
                     /inference="COORDINATES: protein motif:HMM:NF014643.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0110001 - toxin-antitoxin complex
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system Phd/YefM family
                     antitoxin"
                     /protein_id="HRV98510.1"
                     /translation="MITMTSTETRRTWGRVVEKARKNPVVVEKDGRPVVAVISYEDFE
                     RFQELEDRIWGERASAVREKGDYIGTEESMKLLQRGLEE"
     gene            complement(1528..>2557)
                     /locus_tag="P5201_07980"
     CDS             complement(1528..>2557)
                     /locus_tag="P5201_07980"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0052621 - diguanylate cyclase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="sensor domain-containing diguanylate cyclase"
                     /protein_id="HRV98511.1"
                     /translation="YRQIATISEVGQRIASHLDLESSLATIQNAINNLMDASTVILAL
                     VDEEKQLLDYRYVICRGERRESFTRPLDMQSFGCWCVRNRSDIIIGDLESEYSRYVPS
                     YKGLVFEGVPESSLVYIPLILEERVVGMLSVQSTRQHAYDQQKVKVLHAIGGYIAISI
                     ENSRLFSQIERLASTDELTALPNRRTVMEELAKVCRRTRRYGWVSGVVMADVDHFKRI
                     NDMYGHDIGDVALRSIAGALASSVRESDMVGRFGGEEFVVVLQETDSKGALLLAERLR
                     KTVEGLEISGPHGESLRVTASFGVSVVSQKDPDCHPVLKRADDALYEAKATGRNRVCS
                     RLPEDGDA"
BASE COUNT          586 a          752 c          593 g          626 t
ORIGIN      
        1 aaccttgaag gagacaaaag gattgatccg cagaatggct ttaccgtcta ttccctcgtc
       61 atgactgccg ccagtcaact ccagattctc gaacttgttg actcgaagca acagcaagca
      121 aaatcgctcg aagttcttgg gcgtcataga acgaaggtag cttaaaacac gctgtctata
      181 gtctacgttt attgaagagt catcgtcgat ctcattctct tcgccatccg tatcatccaa
      241 atcgttttct tgaatctgag gaactccgtt cttctttttc ctcttttggg cattgatgtc
      301 cacccatttt aggaaaatag cgcgagcctc ttcaagatcg atacgttttt cttccccgat
      361 tttcgtcagc ttccaaacgc cgcgaagaga cccgtcgata tacccctccc agaccagata
      421 ctgcctcgcc cagcacacct gattatgaaa cttggaagct cccgacttga tagtctcgtt
      481 caattttgat tccgggatgt tgagagtttg agcgacacaa ttcgaaactt cctgcggagt
      541 tccggatcct ccaagcttcc tgagcgcatc caacacagga ccaaaccact gtacgaactc
      601 cgaacgtctt tttgccatcc ggaatcactt ccttgccttc agtgataaag gggatctgga
      661 gagcggggga tgagcctcgg agccgtgcca ttcgacggcg gccacggtca caagcgcgtc
      721 gggcagtatc cctcggacga ccgtctcggg gtgcagatct tcgagtcgca aagcgtcacc
      781 ttccttgcag gaatgagttc gcacacaaga aacacgcgtt cgctcttttc gatgcggagt
      841 ttcaggccct cttcttctga gaaaagagga tgatacgaat tccttacatt ttctttctct
      901 tgaacaccct atacacttca tcgtcgttgc gcttatcgat cacgtcgatc atgagcgtgt
      961 cattcacaac cctgtagacc acgcgaaatt cccccgcgtc ttttcgacga aagcctggga
     1021 aacccttcat cgcaagagaa tcgttgggtt ccggatcgcg agtaaggtca tgcaaggcta
     1081 tcgctatctg tttgaactgc tttggctgca agttaaagta aaatttagca gcatctctgg
     1141 aaaaatcaat tttaaggatc attcttccaa gccgcgttgc agcagcttca tgctttcttc
     1201 cgtaccgata tagtctccct tttcacggac tgcgctcgct ctttcccccc agatgcgatc
     1261 ctcaagctct tgaaaacgtt caaaatcctc gtaggagatg acggcaacca ctggccggcc
     1321 gtctttttcc accactacag ggttttttct ggctttttcg acaacgcggc cccaagtacg
     1381 ccgagtctcc gtggatgtca tcgtaatcat tgtttatccc cctctaatgc tcactatgaa
     1441 cactttgatc aatataagca ttatagcatc ttaaaaaata catgtacctc cgttcctcca
     1501 tcatgcaaaa agaaacgagc atgctcacta tgcgtcccca tcctcgggca gacggctaca
     1561 gactcggttt cttccagtag ccttggcctc gtagagggca tcatccgcac gcttcagcac
     1621 ggggtggcag tcggggtcct tctgcgacac cacagagacg ccgaaactcg ccgttacccg
     1681 caggctctca ccgtggggtc ccgatatttc gagtccttca actgttttcc ggagacgttc
     1741 ggctaaaaga agcgctccct tgctgtcagt ctcctgcaga acgacgacga actcctcccc
     1801 gccgaaccgc ccgaccatat cgctctcccg tacgcttgag gcgagcgcgc cggcgatgga
     1861 acggagcgcg acatcgccga tatcgtggcc gtacatgtcg ttgatccgtt tgaaatgatc
     1921 cacatcggcc atcaccaccc ccgaaaccca cccgtaacgt ctcgtcctcc ggcagacttt
     1981 cgccagctcc tccatcacag tccgtcggtt cggaagagcg gtgagctcgt ccgtcgaggc
     2041 cagacgctca atctgactga aaagacgcga gttctcgatg gaaatggcga tgtagccgcc
     2101 gatagcgtga agaaccttca ccttctgctg atcgtaggcg tgctggcggg tgctctgcac
     2161 gctcagcatt cccacgactc tctcttcgag aataagcggg atgtagacca gcgacgattc
     2221 aggaacaccc tcgaaaacca gtcccttata ggagggaacg taccggctgt actccgactc
     2281 cagatcgccg atgatgatat cgctccggtt tctgacacac cagcacccga acgactgcat
     2341 atccagagga cgggtgaaag actcgcggcg ctctccacgg caaatgacgt accggtagtc
     2401 caggagctgt ttttcctcgt ccacaagcgc gagaatcacc gtcgacgcat ccatcaggtt
     2461 attgatggcg ttttgaatcg ttgcgagcga gctctccaga tccaaatgcg acgcgatgcg
     2521 ttgaccgact tcgctgatcg tagcgatctg ccggtag
//
LOCUS       DAOTIL010000357         2549 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminobacteriaceae bacterium isolate AMR_MDS_3026
            NODE_48264_length_2549_cov_3.997193, whole genome shotgun sequence.
ACCESSION   DAOTIL010000357 DAOTIL010000000
VERSION     DAOTIL010000357.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868050
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminobacteriaceae bacterium (wastewater metagenome)
  ORGANISM  Aminobacteriaceae bacterium
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae.
REFERENCE   1  (bases 1 to 2549)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2549)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:19:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,224
            CDSs (total)                      :: 3,198
            Genes (coding)                    :: 3,184
            CDSs (with protein)               :: 3,184
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2549
                     /organism="Aminobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_48264_length_2549_cov_3.997193"
                     /isolate="AMR_MDS_3026"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:3034609"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..952)
                     /locus_tag="P5201_07985"
     CDS             complement(<1..952)
                     /locus_tag="P5201_07985"
                     /inference="COORDINATES: protein motif:HMM:NF015983.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HRV98512.1"
                     /translation="MTGNDSRTFAVMAKVVGGFCNLQCEYCYYTEKTKLLAQTRRLMP
                     DDVLEAYVVENLRIHGKDAVVEFAWHGGEPLLAGIDFFRRAMGLERKYGKGRKIVNTV
                     QTNATLLNDEWRQFFAKHDFLLGVSVDGPEALHDAYRRGPDGGSFSRAMAGIELLRKH
                     GIRFNTLTTVNAANEKHPEEVYDFLRRYTDYMQFLPVVESDAAFFEIEDGQRFATPPG
                     IRSMFLQHSMAPFSASAEGYGHFLVGVFDRWKELDYGRKHVQIFETTAGNMRGVPSSL
                     CVHNPLCGHGASVEVDGAVYSCDHYAFPNYMLGNILETPLD"
     gene            complement(957..>2549)
                     /locus_tag="P5201_07990"
     CDS             complement(957..>2549)
                     /locus_tag="P5201_07990"
                     /inference="COORDINATES: protein motif:HMM:NF013080.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
                     /protein_id="HRV98513.1"
                     /translation="DQERYMKDYPAGTAYEARELLASLGATFEKHYTCATMSTSSRSV
                     IFTGKHITDTKMIDNTDFPWQGPLSKEMTTVGDIMRTAGFYSALKGKWHLGNASTLED
                     VEDELTDLDEHGFSDWDVDGDYIGTMWEGYNKDEEIVTDTVAWLRNVGAVQNAAGKSF
                     FLAANLINPHDIMYFCVDEEFVGPVPVGGAPDDPLYTKTYDEPLPSSWNRDVSDGTVP
                     AAVSIFKNFMNKQTGVLSTEEEWKETQDYYFNCIQDNDNHLIDLLLAVRDMGMLDNSI
                     IFFTSDHGEMGGEHGLKGKGGLLFERNMHVPLIVYHPDYEGGHFVNAVTSHIDLATTF
                     IHMSGLSAERKAEVAEGLPGKNLMELIDGTSQSVRRGALFCNELISTTMARAVRDVNG
                     AIQYYTFDTTVRGLVRAAITERYKFARYFGGNFNTPTTMEALLRDNDIELYDLENDPG
                     ELNNLALDPEANAELIMEMNALLNELIASEIGEDNGDEFHEMIAAYNATLRKESGGCS
                     TASVPAGLLLLLALLPLLRRRK"
BASE COUNT          443 a          785 c          752 g          569 t
ORIGIN      
        1 cgtcgagcgg cgtttcgagg atattcccca acatgtagtt cgggaaagcg tagtggtcgc
       61 acgagtagac cgcgccgtcg acctcgacgg acgctccgtg cccgcacagg gggttgtgga
      121 cgcacagact gctgggcacg ccgcgcatgt tgccggcagt cgtctcgaaa atctgcacgt
      181 gcttcctccc gtagtccagc tccttccatc ggtcgaagac gccgacaaga aagtgcccgt
      241 acccttccgc acttgctgaa aacggcgcca tcgagtgttg cagaaacatg ctcctgatcc
      301 ccggaggcgt cgcgaaccgc tgcccgtcct cgatctcgaa aaaagcggcg tcgctctcga
      361 ccacgggcag aaactgcatg tagtcggtgt atctgcggag gaagtcgtag acctcctccg
      421 gatgtttttc gttcgccgcg ttgacggtcg tcagcgtgtt gaagcgtatt ccgtgtttcc
      481 gaagcagttc gattcccgcc atcgcgcggc tgaacgaccc tccgtccggt cccctccggt
      541 aggcgtcgtg gagcgcttcc gggccgtcga cgctcacccc cagcagaaag tcgtgcttcg
      601 cgaagaattg gcgccactca tcgttcagca gcgtcgcgtt ggtctgcacg gtgttgacga
      661 tcttcctgcc ctttccgtac tttcgttcca ggcccatcgc ccttctgaag aaatcgatcc
      721 cggcaagaag gggctcgccg ccgtgccagg cgaactcgac gacggcgtcc ttcccgtgga
      781 tacgcaggtt ttccacgacg tatgcctcaa gcacgtcgtc gggcatcagg cggcgcgtct
      841 gcgcgaggag cttcgtcttt tcggtgtagt agcagtactc gcattgcaga ttgcagaaac
      901 cgccgacgac cttggccatc acggcgaagg tcctcgaatc gtttcccgtc atctgtttac
      961 ttcctgcggc ggaggagcgg cagcagcgcc aacaggagca gaagtcccgc cggaacggag
     1021 gccgtggagc acccgccgct ttctttacgc agcgttgcgt tgtacgccgc gatcatctcg
     1081 tggaactcgt cgccgttgtc ctcgcctatc tccgatgcga tcagctcgtt cagcagcgcg
     1141 ttcatctcca tgatcagctc cgcgttcgcc tccgggtcga gagcaaggtt gttcagctcg
     1201 cccggatcgt tctccagatc gtacagttcg atgtcgttat cgcgcagcag cgcttccatt
     1261 gtggtcggcg tgttgaagtt tccgccgaag tagcgggcaa acttgtagcg ctccgtgatc
     1321 gccgcgcgca caaggccgcg gacggtcgtg tcgaaggtgt agtattggat agcgccattc
     1381 acatccctga cggcgcgcgc catcgtcgtg gagatcagct cgttgcagaa gagcgcgcct
     1441 ctccgcaccg actgcgacgt accgtcgatc agttccatca gattctttcc ggggagccct
     1501 tcggcgacct ccgcctttcg ttcggcgctc aagccgctca tgtggatgaa ggtcgtcgcg
     1561 aggtcgatat ggctggtgac cgcgttcacg aaatgccctc cctcgtagtc cgggtggtag
     1621 acgatgagcg ggacgtgcat gtttcgctcg aagagcagac cgcccttccc tttcagtcca
     1681 tgttctccgc ccatctctcc gtggtcggag gtgaagaaga tgatcgagtt gtccagcatc
     1741 cccatatccc tcacggccag aaggaggtcg atcaggtggt tgtcgttgtc ctggatgcag
     1801 ttgaagtagt agtcctgcgt ctccttccac tcctcttcgg tgctcagaac accggtctgc
     1861 ttgttcatga agtttttgaa gatggaaaca gccgcaggaa ccgtcccgtc gctcacatcc
     1921 ctgttccatg aggacggcag cggctcgtcg taggtctttg tgtacagggg gtcgtccgga
     1981 gccccgccga cgggaacggg accgacgaac tcctcgtcga cgcagaaata catgatgtcg
     2041 tgcggattga tcaggttggc cgcgaggaag aaggacttcc cggcggcatt ttgaacggcc
     2101 cccacgttgc gaagccatgc gaccgtatcg gtgacgatct cttcgtcctt gttgtacccc
     2161 tcccacatcg tgccgatata gtcgccgtcg acatcccagt cggagaatcc gtgctcgtca
     2221 aggtcggtca gctcgtcctc cacatcttca agcgtgcttg cgttccccag gtgccacttc
     2281 cccttgagcg cgctgtagaa tcccgccgtg cgcattatgt cgccgactgt cgtcatctcc
     2341 ttggaaagag ggccctgcca cggaaaatcc gtgttgtcga tcatcttcgt atcggtgatg
     2401 tgttttcccg tgaagatgac ggaacgggac gatgtggaca tggtggcgca ggtatagtgc
     2461 ttctcgaagg tggcgccgag cgaggccagc agctcccgag cctcgtaggc cgtgccggcg
     2521 gggtagtcct tcatgtagcg ttcctggtc
//
LOCUS       DAOTIL010000358         2543 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminobacteriaceae bacterium isolate AMR_MDS_3026
            NODE_48430_length_2543_cov_3.664791, whole genome shotgun sequence.
ACCESSION   DAOTIL010000358 DAOTIL010000000
VERSION     DAOTIL010000358.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868050
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminobacteriaceae bacterium (wastewater metagenome)
  ORGANISM  Aminobacteriaceae bacterium
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae.
REFERENCE   1  (bases 1 to 2543)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2543)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:19:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,224
            CDSs (total)                      :: 3,198
            Genes (coding)                    :: 3,184
            CDSs (with protein)               :: 3,184
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2543
                     /organism="Aminobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_48430_length_2543_cov_3.664791"
                     /isolate="AMR_MDS_3026"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:3034609"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..760)
                     /locus_tag="P5201_07995"
     CDS             complement(<1..760)
                     /locus_tag="P5201_07995"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HRV98514.1"
                     /translation="MKSKRIGFLFFFLPVALWLGLLIVLPHLELLRLSFTSTRESGFT
                     LANYFAFFNEPIYWLTFVRTALYSILVTFIVMVVALPVAFYITKVAHVRVSGFLMVLV
                     LVPFWVSELIRVYGWIILLRESGVLNNFLVSIGLFEKPVEMLYNDATMIMGLVYTSML
                     FMVVPVIGVMDALDDSLIEAAYDLGASKTAIWRTIIIPHCTPGMVSGGIIVFMLVLGS
                     YLTPNLMGGKNSLWFTEQIYNQIILYFNWNQGAAF"
     gene            complement(750..1862)
                     /locus_tag="P5201_08000"
     CDS             complement(750..1862)
                     /locus_tag="P5201_08000"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HRV98515.1"
                     /translation="MRNALSVQNLVKRFGTFTAVNNVSFDVEDGKFFSILGPSGCGKT
                     TLLRMIAGFYQQDEGTILFNGQDMEGVAPNKRPVNLVFQNLALFPMMNVHENVAFGLR
                     RRGEKGAAVERKVKDMLERVGLPESGKKEVSQLSGGQKQRIAIARCLVLEPTVLLLDE
                     PLGALDAKLREHMKVELKTLQAKVGTTFVYITHDQSEAMVMSDRVAVMNNGAFEQIGT
                     PQELYHAPSSSFVAQFVGANNKLSVSLRRGEAGELLARYANGLDFRLSPACSEEATDE
                     PFDLFVRPESMVINPPEGDGRFNSLPVTVKAILFDGGNSRLLVSSEGTTDEFTVALPQ
                     TRQFDDIKVRDTIRVGWDPSASLCFRKSDWKLYDEE"
     gene            complement(1946..>2543)
                     /locus_tag="P5201_08005"
     CDS             complement(1946..>2543)
                     /locus_tag="P5201_08005"
                     /inference="COORDINATES: protein motif:HMM:NF024808.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HRV98516.1"
                     /translation="YAGRVSYRLKRPTLIGMGFSLGYDPFSLYTKTEEYQKFLDEMEK
                     VLIAGKPAVKTYWDNGDQLLQLLRSGEVWAASAWEQAGWKLHAENPDIDYIAPTSGAL
                     AWVDTFAIPARSENLEAAYAYINFMLRPENAAFFTNRETYGTASKDAVKHLDPEIKAN
                     FERGLPPEVLAKINWYPTVPAGVEEMEGKTLDKVKAAR"
BASE COUNT          464 a          769 c          834 g          476 t
ORIGIN      
        1 cgaaggccgc cccctggttc cagttgaagt acaggatgat ctggttgtag atctgttcgg
       61 tgaaccagag ggagtttttg ccccccatca ggttcggcgt caggtagctc ccgagcacca
      121 gcatgaagac gataattccc cccgagacca tgccgggggt gcagtgcggg atgatgatcg
      181 tgcgccagat ggccgttttc gacgcgccga ggtcgtaggc cgcctcgatc agcgagtcgt
      241 cgagcgcgtc catcacgccg atgaccggca ccaccatgaa gagcatgctg gtgtacacca
      301 gccccattat catcgtcgcg tcgttgtaga gcatctcgac tggcttctcg aagaggccga
      361 tggagacgag aaagttgttc agcacgccgc tctcgcgaag caggatgatc cagccgtaga
      421 cgcggatcag ctcgctcacc cagaagggga cgagaacgag caccatcagg aagccgctca
      481 cgcgcacatg tgcgaccttc gtgatgtaga aggccacggg gagcgcgacc accatcacga
      541 tgaaggtgac caggatggag tagagcgcgg tgcggacgaa ggtgagccag tagatcggct
      601 cgttgaagaa ggcgaagtag ttggcgagcg tgaagccgct ctcgcgggtg gaggtgaagg
      661 agaggcgcag cagctcgagg tgcgggagga cgatcagcag cccgagccag agggccaccg
      721 gcagaaagaa aaagagaaag ccgatccgtt tactcttcat cgtacagctt ccagtcgctc
      781 ttccggaaac agagcgaggc cgacggatcc caccccacgc ggatggtatc gcgtactttg
      841 atgtcgtcga actgtcgcgt ctgcggcagc gccacggtga actcgtcggt cgtcccctcg
      901 gacgagacca gcaggcggct gtttcccccg tcgaagagga tcgccttcac cgtcacgggc
      961 agcgagttga agcggccgtc cccttcgggc gggttgatca ccatcgactc cgggcggacg
     1021 aaaaggtcga acggctcgtc cgttgcctct tccgaacagg ccggcgagag gcggaagtcg
     1081 agcccgttcg cgtaccgtgc gagcagctcg cccgcttcgc ccctgcggag cgatactgag
     1141 agtttattgt tggcgccgac gaactgcgca acgaacgagg aagagggcgc gtgatacagc
     1201 tcctgcggcg tgccgatctg ctcgaaagcg ccgttgttca tcacggccac cctgtcgctc
     1261 atcaccatgg cttccgactg gtcgtgcgtg atgtagacga acgtcgtccc caccttggcc
     1321 tggagcgtct tcagctccac cttcatgtgc tcgcggagct tcgcgtcgag cgcgccgagc
     1381 ggttcgtcga gcagcagcac cgtcggctcc agcaccaggc agcgcgcgat ggcgatgcgc
     1441 tgcttctgtc cgcccgagag ctgactgact tccttcttcc cggactccgg caggccgacc
     1501 cgttcgagca tgtccttcac cttccgctcc acggcggccc ccttctcgcc gcggcgccga
     1561 aggccgaacg cgacgttttc gtggacgttc atcatcggga agagggcgag gttctggaag
     1621 acgaggttca cggggcgctt gttcggggcc acgccctcca tgtcctgccc gttgaagagg
     1681 atggttccct cgtcctgctg gtagaagccg gcgatcatcc ggagcagggt ggtcttgccg
     1741 caccccgagg ggccgaggat cgagaagaac ttgccgtcct ccacgtcgaa ggagacgttg
     1801 ttcaccgcgg taaaagtgcc gaatcgtttc acgaggttct gtactgaaag agcgttgcgc
     1861 atacggcggt cacctctgaa tgtgcagcag gcggcggcct cttcgcgaag accgccgcct
     1921 gccggcagga tagatgacgg ctgcgttatc tggccgcctt tactttatcg agcgtctttc
     1981 cttccatctc ctccacgccc gccggaacgg tggggtacca gttgatcttc gcgagcacct
     2041 cgggcggcaa gccgcgctcg aagttcgcct tgatctccgg gtcgagatgc ttcacggcgt
     2101 ccttggaggc tgtgccgtag gtctcgcggt tggtgaagaa ggccgcgttt tcggggcgga
     2161 gcatgaagtt gatgtaggcg taggccgctt ccaggttctc ggagcgcgcc ggaatggcga
     2221 aggtgtcgac ccaggcgagc gcgccgctcg tgggggcgat gtagtcgatg tcggggttct
     2281 cggcgtggag cttccatccg gcctgctccc acgcggacgc agcccagact tcgccggagc
     2341 ggagcagctg gagcagctgg tcgccgttat cccagtacgt cttcacggcg ggcttgccgg
     2401 cgatgaggac cttctccatc tcgtcgagga acttctgata ctcttcggtc ttggtataca
     2461 gggagaacgg gtcgtacccc agcgagaagc ccatgccgat cagggtgggg cgcttcagcc
     2521 ggtaggagac gcgcccggcg tac
//
LOCUS       DAOTIL010000359         2537 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminobacteriaceae bacterium isolate AMR_MDS_3026
            NODE_48574_length_2537_cov_4.016922, whole genome shotgun sequence.
ACCESSION   DAOTIL010000359 DAOTIL010000000
VERSION     DAOTIL010000359.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868050
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminobacteriaceae bacterium (wastewater metagenome)
  ORGANISM  Aminobacteriaceae bacterium
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae.
REFERENCE   1  (bases 1 to 2537)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2537)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:19:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,224
            CDSs (total)                      :: 3,198
            Genes (coding)                    :: 3,184
            CDSs (with protein)               :: 3,184
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2537
                     /organism="Aminobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_48574_length_2537_cov_4.016922"
                     /isolate="AMR_MDS_3026"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:3034609"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1818
                     /locus_tag="P5201_08010"
     CDS             <1..1818
                     /locus_tag="P5201_08010"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRV98517.1"
                     /translation="FDDPSMDVLEHLLRSSPGNLTLFFTSRAASSFRGVSLVRDAARN
                     GRIALLEVRLSCFSRRESDDFCAALLPSRPFSTEELELVFRQTEGLPLFLSELLRAVE
                     KGLPLYEAPRHLGDAIEGHLCHIDEEQRRLLEAISIFPQGAEWTVVRRLLGLSGARLA
                     ALSESLRGRGLLEENRGEGESLLLEFRHGKVREHVYAFMSEGRRRTLHESAARLLMDE
                     FSVRPWEDLACSRIIHHCKRGGLRLEELEYMIRRLKYHIKLNYELFPLLDDHILKACA
                     TSLDNRSSTADDLEAARELLRALRKEKEGATPRFAALERAYFALRGGYYLWWGDYDQG
                     KALVEEGLARAAETGDVALQADCLQHLCYYAIQREDGELLCSAASRLSEAAERCGDAS
                     LGAMARRFSGMSLLFLGKFEEAERLLQESVAQFRRMEYLDSPYTLQVLAGNCYLGEIR
                     YRTGRVREALSVFCRCVVECEEHGLYRGLCLFCSNAALAAFDLGEDETAKEYLRKARH
                     YFEGSELRRANAVGLALLALFALREGKDDEAISFWESAGNLCVSLRKKSWVAACCRIR
                     DVLERELEAGHLSGQRKNTTTLNAFSANCRTFKEVCGDD"
     gene            complement(1827..1994)
                     /locus_tag="P5201_08015"
     CDS             complement(1827..1994)
                     /locus_tag="P5201_08015"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRV98518.1"
                     /translation="MEIHLHTEEIEELKSLMEEDEFTGAVEVFAREMLLEGMSYHKAR
                     QPGGYLHPEEK"
     gene            complement(2190..>2537)
                     /locus_tag="P5201_08020"
     CDS             complement(2190..>2537)
                     /locus_tag="P5201_08020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006301823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0071705 - nitrogen compound transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BCCT family transporter"
                     /protein_id="HRV98519.1"
                     /translation="ILSWVFVGTVFISIVTMADSMTSTVASLSTTAAHQEGKEPPATL
                     KIFWGAVMSSVAIINLISGSGGGEISGIDATKQISTAAGFPILFLMMLMAFTICWMII
                     RHSRGEKVLLVKE"
BASE COUNT          507 a          703 c          753 g          574 t
ORIGIN      
        1 tttgacgatc cttcgatgga tgtgcttgaa catctgctcc gttcgtcacc ggggaatctc
       61 acgttgttct tcacctcgag ggcggcgtcc tccttccgcg gcgtttccct tgtgcgggat
      121 gccgcccgaa acgggcgtat cgccctcctc gaagtccggc tctcgtgctt ctcccgaaga
      181 gagtccgatg acttctgcgc agcgttgctt ccttcgcgcc cattttctac agaggaactg
      241 gaattggtct tccgccagac cgaggggctg ccgctcttcc tctccgaact tctgagggct
      301 gtcgagaaag gcctgccgct ttacgaagcg ccccggcatc tcggagatgc catagagggg
      361 catctgtgcc atatagacga agaacagcgc aggcttctgg aggcgatctc catctttccg
      421 cagggcgccg agtggacagt ggtgcgaaga cttctcgggc tgtccggcgc acgattggct
      481 gcgctgtccg agagtttgag agggagagga ttgctcgagg aaaaccgcgg cgagggagaa
      541 tcgctccttt tggagtttcg tcacggcaaa gtaagagagc atgtgtacgc tttcatgtcc
      601 gaaggacgga ggcgaacgtt gcacgagagc gcagcccgcc ttctcatgga cgaattttcc
      661 gttcgcccgt gggaggacct cgcctgctca aggatcatcc atcactgcaa acgaggaggg
      721 ctgcgcctgg aggaactcga atatatgatc cggaggctca agtatcatat caagttgaac
      781 tacgaactct ttccccttct cgacgaccat attctgaaag cctgtgcgac ctctctggac
      841 aatcgctcga gcacggcgga tgatctggaa gccgcacggg agctgctccg cgcgctccgg
      901 aaggaaaagg agggcgccac gccgcgcttt gccgctctcg aacgggcgta cttcgcgctt
      961 cgaggcgggt actacctctg gtggggcgat tacgatcagg gaaaggcgct cgtcgaggag
     1021 gggcttgccc gcgctgcaga aacgggtgac gtcgccttgc aggccgactg ccttcagcat
     1081 ctttgctact acgctattca aagagaggac ggcgagctgc tgtgctccgc agcttccagg
     1141 ctgagcgaag cagcggaacg ctgcggcgat gcgtcgcttg gcgccatggc gcgcaggttt
     1201 tccgggatgt cgctgctctt tctgggaaag ttcgaagaag cggagcgctt gttgcaggag
     1261 tctgtcgctc aattccggcg gatggagtat ctcgactctc cgtacacgct ccaggtgctt
     1321 gcaggaaact gctacctcgg agagatacgc tatcgtaccg gacgcgttcg cgaagctctt
     1381 tccgtttttt gccggtgcgt cgttgaatgc gaggaacacg gcctgtaccg tgggctttgc
     1441 ctcttttgca gcaacgcggc gctcgccgcg ttcgatcttg gggaagacga gacggcgaag
     1501 gagtatctcc gcaaggcgag gcactacttc gagggcagcg agctgcggcg ggcgaacgct
     1561 gtcggtttag cgcttcttgc gctcttcgcc ctccgcgaag gaaaagatga cgaggcgatc
     1621 tccttctggg aatcggccgg gaatctgtgc gtctcgttac gaaaaaagtc ctgggtcgct
     1681 gcgtgttgcc ggatacgtga cgtgctcgag cgggaactcg aagcagggca tttgtcaggg
     1741 cagcgaaaaa acacaacaac attaaacgct ttttcagcga attgcagaac gttcaaagag
     1801 gtgtgcggcg acgattaaag gagtccttac ttttcttccg gatgcagata ccctccgggt
     1861 tgtcgggcct tatgatagga catgccctcc aaaagcatct ccctcgcgaa aacctccaca
     1921 gctcctgtga attcatcttc ctccatcaga cttttcaatt cttctatctc ttctgtgtgc
     1981 agatggattt ccatagccag atcgtccctc cttgttttcc gggtagtact aataatctta
     2041 gtatatttga aatattacgt caagggaaga tgcaggaaat cgctcatctg aatgtttcgc
     2101 ctgtgtggaa gaaacgaaaa acgtccgatg ggcatggaaa aacccgggag ctgcgccccc
     2161 gggccttctt tttcaaatcg ctctttcggt tactccttca cgaggagcac tttctcaccc
     2221 cttgaatgac ggatgatcat ccagcagatg gtgaacgcca tcagcatcat caggaagagg
     2281 atcgggaatc ccgccgcagt ggaaatctgt ttggtcgcgt cgatgccgct gatctcgccg
     2341 ccgccgctgc cgctgatcag gttgatgatc gccacggagg acatcaccgc tccccagaag
     2401 atcttgaggg tcgcgggggg ttcctttccc tcctggtggg cggccgtcgt ggagaggctt
     2461 gctaccgtcg aggtcatgga gtcggccatg gtgacgatcg agatgaagac cgtgccgacg
     2521 aagacccagc tcagtat
//
LOCUS       DAOTIL010000360         2535 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminobacteriaceae bacterium isolate AMR_MDS_3026
            NODE_48639_length_2535_cov_2.764113, whole genome shotgun sequence.
ACCESSION   DAOTIL010000360 DAOTIL010000000
VERSION     DAOTIL010000360.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868050
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminobacteriaceae bacterium (wastewater metagenome)
  ORGANISM  Aminobacteriaceae bacterium
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae.
REFERENCE   1  (bases 1 to 2535)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2535)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:19:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,224
            CDSs (total)                      :: 3,198
            Genes (coding)                    :: 3,184
            CDSs (with protein)               :: 3,184
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2535
                     /organism="Aminobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_48639_length_2535_cov_2.764113"
                     /isolate="AMR_MDS_3026"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:3034609"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..592
                     /locus_tag="P5201_08025"
     CDS             <1..592
                     /locus_tag="P5201_08025"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HRV98520.1"
                     /translation="RDILKALLAPLGFALREAENGREALRLFEEERPCAVLMDMRMPV
                     MDGYEATKRIKASEEGSSVPVIAVTASAFEDDVQNVLKSGADICIRKPLSPEDVYEAL
                     GKLLDIRFLYADEPRTADTEQEPPPSMEALAALPEELIKAMRRAVEDGDIDELHTLVD
                     RVREMNREVAEGLQKLVRQYDYAALSSLLSDGNRER"
     gene            627..>2535
                     /locus_tag="P5201_08030"
     CDS             627..>2535
                     /locus_tag="P5201_08030"
                     /inference="COORDINATES: protein motif:HMM:NF019874.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cache domain-containing protein"
                     /protein_id="HRV98521.1"
                     /translation="MKKDGLTAKKNGAFRNMKLGSRIILAVGALLLPAILIFAGIVLR
                     RIDADATASIEALRVSETERIRSDLRGYVDIAWRILSDKYEQSTQTESIIQSYGDQLR
                     SVVDIAENIVRQIMKSAEEGQFSREEAKRQAMIALRALRFDDGLGYVWVNDTSLPYPS
                     FLMHPTQPELEGVLMKGPDFDKAMGSEKNIGKSILEACALDGEGFVQYLWSKPTRDGL
                     IPDIPKLSYVRSIKEWGWIIGTGVYVDDAREYAVRQALDIIANMRYAGGAGYFWVTDT
                     HRPNPRPVIHPTLDAAENELPGITPDDKYYDTARKLVEEVGVQAADVCEANGEGFVRY
                     LWPRIGTDGALELAPKETFVRLFEPLQWIIGTGVHLDDVDRAVALRQAESKRQRNIVL
                     RGTLFAALLLSALAFVLLYFVVRGIVRPIVQLTAAADMVASGDMDSPLSETGPREIRS
                     LSRSFNRMTKELKEQMSALRESEERFRRLAENSPDLIFRISLRDGRYEYISPAITRLF
                     GYDRDEVYSNPLFIRQIIHPDFADTFNNARQRLLQGAQSVTFEYKAFGKSGEEHWMHQ
                     RNVLVFGDEGRPVALEAIVSDITARKTAEEALDSYREHLEEQVRERTAELKVAKEAAE
                     QANRTKSAFLAN"
BASE COUNT          568 a          750 c          762 g          455 t
ORIGIN      
        1 ccgcgatatc ctgaaggcgc tgctcgctcc tctcggcttc gccctcagag aggcggaaaa
       61 cggccgggag gcgcttcggc tgttcgagga ggaacgcccc tgcgccgtgc tgatggatat
      121 gcggatgccc gtgatggacg gctatgaagc cacaaagaga atcaaggcga gcgaagaagg
      181 aagttcggtt cccgtcatcg cggtgacggc gagcgccttc gaggacgacg ttcagaacgt
      241 gctgaagagc ggcgccgata tctgcatacg caaaccgctc agtcccgagg atgtgtacga
      301 ggcgctggga aagctgctcg acatacgctt cctctacgca gacgagccgc ggacagccga
      361 tactgaacaa gagcctccgc cttccatgga agcgctggcc gcgctgccgg aagagttgat
      421 caaggcgatg agaagggccg tcgaagacgg cgatatcgac gaactgcaca cgctcgtcga
      481 ccgggtgcgg gagatgaacc gggaggttgc cgaagggttg cagaagctcg tacgccaata
      541 cgactacgcg gcgctttctt ctcttctctc ggacggcaac cgggagagat gaaactcgtg
      601 atcgggcgca gccaggagga actcgaatga aaaaggacgg attgaccgct aagaaaaacg
      661 gcgcgttccg gaacatgaag ctcggcagcc gaatcattct ggccgtcggg gcgctgctgt
      721 tgcctgccat cctcatcttc gccggaatcg tcctccgccg catcgacgcc gacgcaacag
      781 cctccatcga ggcgctgcga gtctccgaga ccgaacgcat ccgctccgac ctgcgcggct
      841 acgtcgacat cgcatggcgc attctctccg acaagtacga acagtcgacg cagaccgaat
      901 ctatcatcca gtcgtacgga gaccagctcc gctcggtggt cgacatcgcg gaaaacatcg
      961 tccggcagat catgaagtcg gcggaagagg ggcaattttc ccgtgaagag gcaaaacggc
     1021 aggctatgat tgcccttcgg gccctgcgtt tcgacgacgg cctcggctac gtctgggtca
     1081 acgacacatc tctcccctat ccttcgtttc ttatgcaccc tacccaaccc gaactggaag
     1141 gcgtcttgat gaagggcccg gacttcgata aagcgatggg cagcgaaaag aacataggca
     1201 aatcgatatt agaagcatgc gcactggacg gagagggttt tgtgcaatac ctgtggtcca
     1261 aacctaccag ggacgggctg attcctgata taccaaaact ctcctacgtc agatcgataa
     1321 aggagtgggg gtggatcatc ggcaccgggg tctacgtgga cgacgcccgg gagtacgccg
     1381 tccggcaggc gctggatatc atcgcaaaca tgcgttatgc cggaggcgca gggtacttct
     1441 gggtcaccga cacgcaccgg cccaacccga ggccggtcat tcatccgacc ctcgacgccg
     1501 ccgaaaatga actgccgggg atcacgccgg acgacaaata ctacgatacc gcgcgcaagt
     1561 tggtcgaaga ggtcggagtc caagcggccg acgtgtgcga agcgaacggc gaggggttcg
     1621 tgcggtacct gtggccccga atagggacgg acggagctct tgaactcgcg ccgaaggaga
     1681 ccttcgtgcg cctcttcgaa ccgctccaat ggatcatcgg gacgggcgtg catctggacg
     1741 acgtcgacag ggccgtcgcc cttcgccagg ccgaaagcaa acggcagcga aacatcgtgc
     1801 ttcgcggcac gctcttcgct gcgctgctgc tctccgccct cgccttcgtt ctgctctatt
     1861 tcgtggtgcg gggcatcgtc cggccgatcg ttcagctcac cgccgccgcc gatatggtgg
     1921 cctccgggga tatggactcc cccctctccg aaaccggacc gcgcgagata cgctctcttt
     1981 ctcgctcttt caacaggatg accaaagagc tgaaagagca aatgtcagcc cttcgcgaga
     2041 gtgaggagcg attccgccga ctggcggaga actcccccga cttgatcttc agaatatcgt
     2101 tgcgcgacgg caggtacgag tatatcagtc ctgccattac acgcttgttc ggctacgaca
     2161 gggacgaagt gtattccaat ccgcttttta tccgtcagat tattcatccg gactttgccg
     2221 acacgttcaa taatgcgcgg caacggcttc ttcagggagc acaatcagtg acctttgagt
     2281 ataaggcgtt tggaaaatcc ggagaagagc actggatgca tcagcgcaac gtgctcgtct
     2341 tcggggatga aggaaggcct gtcgccctgg aagccatcgt cagcgacatc accgcccgga
     2401 agacagcgga agaggctttg gacagctatc gtgagcacct cgaggagcag gttcgcgaac
     2461 gaactgccga actgaaggtc gcaaaagagg cggcggaaca ggccaaccgc accaagagcg
     2521 ccttcctcgc caaca
//
LOCUS       DAOTIL010000361         2526 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminobacteriaceae bacterium isolate AMR_MDS_3026
            NODE_48882_length_2526_cov_3.306758, whole genome shotgun sequence.
ACCESSION   DAOTIL010000361 DAOTIL010000000
VERSION     DAOTIL010000361.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868050
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminobacteriaceae bacterium (wastewater metagenome)
  ORGANISM  Aminobacteriaceae bacterium
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae.
REFERENCE   1  (bases 1 to 2526)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2526)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:19:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,224
            CDSs (total)                      :: 3,198
            Genes (coding)                    :: 3,184
            CDSs (with protein)               :: 3,184
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2526
                     /organism="Aminobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_48882_length_2526_cov_3.306758"
                     /isolate="AMR_MDS_3026"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:3034609"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..322)
                     /gene="grdA"
                     /locus_tag="P5201_08035"
     CDS             complement(<1..322)
                     /gene="grdA"
                     /locus_tag="P5201_08035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_084002417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_except=(pos:complement(191..193),aa:Sec)
                     /transl_table=11
                     /product="glycine/sarcosine/betaine reductase complex
                     selenoprotein A"
                     /protein_id="HRV98522.1"
                     /translation="MGKLTGKKLLLLGERDGVPGPAMEACLKASGASIEFSVTECFVU
                     TAAGAMDLQNQQRIKDAAEKFGAENVVVILGSSDAEGAEIYAETVSNGDPTYAGPLAG
                     VSLGL"
     gene            complement(469..1359)
                     /locus_tag="P5201_08040"
     CDS             complement(469..1359)
                     /locus_tag="P5201_08040"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRV98523.1"
                     /translation="MFSRLFGQFLLQEGFVTPSALSSALGRLGEVRPLIGMLALAQGY
                     MSPDQVVEINEMQKRVDRRFGELAIEKGYLSVDQLESIISAQKSEHVLLGQILVQDGI
                     LSHDGFLLVLEKYRKNAGLSEEGYEAVKANDVEGVVSSILVGQPGAGKAVVRSWATVF
                     MRSVIRFIDPGAAIDPLAEEKYKNLRGFIQRMKGDLRVSVFIAAEDEVFVDLAQRFTD
                     FDIDSFDEMASAAVGEFLNLANGLFTVNCSDEGTELDMLPQEYVESIASLPIGRPDAL
                     LPLLLPKGILWAGFCPGLSR"
     gene            complement(1359..1739)
                     /locus_tag="P5201_08045"
     CDS             complement(1359..1739)
                     /locus_tag="P5201_08045"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HRV98524.1"
                     /translation="MEKKYTALLCDDSAVVRKMLRHILNESPVGTVFEAIDGNQAVEM
                     YRLHRPDIVFMDIVMPGKTGIQALSEIRAHDPSARVIMASSTGTSKNLKAAIDSGAVD
                     FIQKPFEREAVLKLIRRILEGEWE"
     gene            2065..>2526
                     /locus_tag="P5201_08050"
     CDS             2065..>2526
                     /locus_tag="P5201_08050"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRV98525.1"
                     /translation="MRNAVFLGPSGGGKSEISINMALRAAAEDGPAVHFFDMDQTKPL
                     FRSRACRDLLERSGVVFHSGAEFLDSPVIPDGVADFLRDPACRCILDVGGNPAGARPL
                     GQLAALREADAAAFYVINVYRPFSDEQSLREDMERIVASARIPAPCVVSNPN"
BASE COUNT          525 a          762 c          704 g          535 t
ORIGIN      
        1 ggagtcccaa cgagactccc gcgagagggc ctgcataggt cggatcgccg ttgctcacgg
       61 tctcggcgta gatctccgcg ccttcagcgt cggaggaacc gaggatgacg acaacgtttt
      121 cagctccgaa cttctcggca gcgtccttga tacgctgctg attctgcagg tccatcgctc
      181 ccgcggcggt tcagacgaag cactccgtca cggagaattc gatggaagcg ccgcttgcct
      241 tcagacatgc ctccatggcg ggtccgggga cgccgtcccg ctcaccgagg agaagcagtt
      301 tcttgcctgt cagtttgccc atttttccac ctcctttctg ctttacaggg tagcgtcaaa
      361 tgaggatttc gtccagagcc ggcggaaaaa atcggcggga aagaaaaaaa cggcgccccg
      421 ccgcgaaggg aggagcaccg tctgcgattt cagtgcacgg aaagcgcgct accgggagag
      481 accggggcag aaccccgccc agagaattcc cttgggaagc aggagcggaa gaagcgcgtc
      541 ggggcgtcct atcggaagcg acgcgatgct ttcgacgtat tcctggggga gcatatccag
      601 ctccgtccct tcgtctgaac agttcaccgt aaacagaccg ttggcgaggt tcaaaaattc
      661 gccgaccgcc gccgacgcca tctcgtcgaa cgagtcgatg tcgaaatcgg taaaacgctg
      721 tgcgagatcg acgaagactt cgtcttcggc tgcgatgaag accgacacgc gaaggtcgcc
      781 cttcatgcgt tgtatgaacc ccctcagatt tttgtacttc tcctccgcca gcgggtcgat
      841 ggcggcgccg gggtcgatga accggatgac gctcctcata aagacggtgg cccagctcct
      901 gaccacagct tttcccgctc caggctgccc tacgagaatg gaggagacca cgccctcgac
      961 gtcgttcgcc ttgaccgcct cgtacccctc ttcggagaga ccggcgttct tgcggtactt
     1021 ttcgagcacc aggaggaagc cgtcgtgcga caggatgccg tcctggacaa gaatctgccc
     1081 gaggagcacg tgttcgctct tctgggcgga gatgatggat tcaagctgat cgacggagag
     1141 gtaccccttc tcgatggcga gttcgccgaa acggcggtcg accctcttct gcatctcgtt
     1201 gatctcgacg acctggtcag ggctcatgta gccctgcgca agcgcgagca tccctataag
     1261 ggggcgcacc tcgccgagcc gccccaaggc gctcgaaaga gcgctgggcg tgacgaaccc
     1321 ctcctggaga agaaactgcc cgaaaagtct tgagaacact actcccactc cccttccaga
     1381 atacggcgta tcagtttgag cacggcctcc cgctcgaacg gtttttgaat aaaatccacc
     1441 gcgccggagt cgatggccgc tttcaagttc ttgctggtac ccgtggacga ggccatgatc
     1501 actcgcgccg acgggtcgtg cgcacgtatt tcggaaagcg cctggatacc tgtcttccct
     1561 ggcatgacga tgtccatgaa aacaatatcc ggccggtgca agcggtacat ctccaccgcc
     1621 tgattcccgt cgatggcttc aaagaccgtt cccacagggc tttcgtttaa aatatgacgc
     1681 aacattttgc gcaccactgc ggagtcgtcg cagagaagcg ctgtgtattt tttttccacg
     1741 gaagaagccc tccctaatat ggagttctct catccaagaa ctctttttga gaattatagc
     1801 atgaagcgca atgtgcaagc agatggatga tgaaatttat tcactaaaga aatatcggat
     1861 atttaaaaaa agttcgcaag ttttttcagc gcgctccgct ttcgcaaggc acgccttaag
     1921 actcctcgaa cacagtgaat acgcatgcaa acgtgagcat tgacaaatgc aggcgggggt
     1981 attagcatcg tttttgaacc gtcttgattt cacagtttat gcctaaatat aaaatatttt
     2041 gatttttatc taggaggcga tggcgtgcgc aacgcggtct ttcttggccc ttccggcgga
     2101 ggcaagtcgg agatctccat caacatggcg ctgcgcgcag ccgcagagga cgggcctgcc
     2161 gttcacttct tcgacatgga ccagacgaag ccgctcttcc gctcccgggc ctgccgcgac
     2221 cttctggagc gctccggcgt cgtcttccat tctggggccg aatttctcga ctctcccgtc
     2281 attccggacg gcgtcgcgga ttttctgcgc gaccccgcct gccgctgcat cctcgacgtg
     2341 gggggcaacc ctgcgggagc tcgtccgctc ggacagctcg ccgccctgcg tgaagccgac
     2401 gcggccgctt tctacgtgat caacgtctac cgcccgttct ccgacgaaca gagtctccgg
     2461 gaggacatgg aacgcatcgt cgcatcggcc cgcatccccg ctccgtgcgt ggtgagcaat
     2521 ccgaac
//
LOCUS       DAOTIL010000362         2524 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminobacteriaceae bacterium isolate AMR_MDS_3026
            NODE_48928_length_2524_cov_3.682463, whole genome shotgun sequence.
ACCESSION   DAOTIL010000362 DAOTIL010000000
VERSION     DAOTIL010000362.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868050
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminobacteriaceae bacterium (wastewater metagenome)
  ORGANISM  Aminobacteriaceae bacterium
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae.
REFERENCE   1  (bases 1 to 2524)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2524)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:19:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,224
            CDSs (total)                      :: 3,198
            Genes (coding)                    :: 3,184
            CDSs (with protein)               :: 3,184
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2524
                     /organism="Aminobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_48928_length_2524_cov_3.682463"
                     /isolate="AMR_MDS_3026"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:3034609"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..322
                     /locus_tag="P5201_08055"
     CDS             <1..322
                     /locus_tag="P5201_08055"
                     /EC_number="4.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009164827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004590 - orotidine-5'-phosphate
                     decarboxylase activity [Evidence IEA];
                     GO_process: GO:0006207 - 'de novo' pyrimidine nucleobase
                     biosynthetic process [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="orotidine 5'-phosphate decarboxylase"
                     /protein_id="HRV98526.1"
                     /translation="PGCSLSDALEKRAALCRETGIDGIVCSPLDLPVVRVAGAGLFTV
                     VPGVRPASADDDQARVATPQKTIANGADYIVVGRPILNAPDRIAAVRSIAASIEEGLK
                     CRSL"
     gene            307..897
                     /gene="pyrE"
                     /locus_tag="P5201_08060"
     CDS             307..897
                     /gene="pyrE"
                     /locus_tag="P5201_08060"
                     /EC_number="2.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013048975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004588 - orotate
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009220 - pyrimidine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="orotate phosphoribosyltransferase"
                     /protein_id="HRV98527.1"
                     /translation="MQESVMLAKLQEMMKESGAHLEGHFLLTSGNHSSDYMQCALLLR
                     FPRYAAFAGEALAEKVRRYSPDLIASPALGGLIIGHEVARALDVPFIFCERREGEMVL
                     SRFPFPEGKRVVVIEDVVTTGVSSGEVGSVLERGGATWVGTGAVIDRSGGKSSLPHVP
                     ESLWKVAFPLWKPEECPLCAKGIPLVKPGSRTKTAP"
     gene            1140..1538
                     /locus_tag="P5201_08065"
     CDS             1140..1538
                     /locus_tag="P5201_08065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRV98528.1"
                     /translation="MKNTTRLLLVSGLLCLLVFLAYLFLFSGQEAPEPLDGDPLRERG
                     MRAAAIGGELVSVAKEQLGALQEEHRADVGELLGELERKTAAVKELLSRKDADEDDLA
                     AAVSALEKALDGVLVVPDNETGNEERRTDS"
     gene            1535..2035
                     /locus_tag="P5201_08070"
     CDS             1535..2035
                     /locus_tag="P5201_08070"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRV98529.1"
                     /translation="MTSVNETAVLARRVNEMLRRYMKVQEDLFKPSLRKILRIPGIYR
                     PIDYAENLRALEELLRELAEVKAAIRREEPDAASPEGKFLGVLRGYVSLMTSAVEKLE
                     NICSRLKERSEGGAYGKDEYKSDMAALREIQKKHLESGVALNEMMKTLSRSDPPKETQ
                     DDESKS"
     gene            2266..>2524
                     /locus_tag="P5201_08075"
     CDS             2266..>2524
                     /locus_tag="P5201_08075"
                     /inference="COORDINATES: protein motif:HMM:NF012610.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HRV98530.1"
                     /translation="MREKKKDQAYETIRTLVLQRRSSDSFVISENSLAAELNMSRTPI
                     REALQRLQMEGFIEIHPHRGIVIPEVSVIEVNETFALRMAIE"
BASE COUNT          519 a          715 c          796 g          494 t
ORIGIN      
        1 ccccgggtgt tcgctttccg acgcgctcga aaaacgcgcc gcgctctgcc gggagaccgg
       61 aatcgacggc atcgtctgct cgccgctcga ccttccggtg gtccgcgtag ccggggccgg
      121 gctcttcacc gtggtcccgg gggttcgccc tgcgagcgcc gacgacgacc aggcgcgcgt
      181 cgccacgccg cagaaaacaa tcgcgaacgg cgcggactac atcgtcgtgg ggcgtccgat
      241 actgaacgct ccggacagaa tcgcggcggt gagaagcatc gccgcgtcga tagaggaggg
      301 gctgaaatgc aggagtctgt aatgctggcg aagttgcagg agatgatgaa ggagagcggc
      361 gcgcaccttg aaggccactt tctgctgact tcggggaacc acagcagcga ctacatgcag
      421 tgcgccctgc tgcttcgctt tccgcgctac gccgccttcg ccggagaggc gcttgcggag
      481 aaggtgcgcc ggtactcgcc cgaccttatc gcctcgcccg ctctcggcgg cctcatcatc
      541 gggcacgagg ttgcgcgcgc gctcgacgtt cccttcatct tctgcgagcg aagagagggc
      601 gagatggtcc tcagccgctt cccgttcccc gaggggaagc gcgtcgtggt gatcgaggac
      661 gtggtcacta ccggcgtctc ctccggcgag gtaggaagcg ttctcgaacg cggcggcgcg
      721 acatgggtcg ggacgggggc cgtcatcgac aggagcggcg ggaagtcctc gctccctcat
      781 gttccggagt cgctttggaa ggtggcgttt ccgttgtgga agcctgaaga gtgtcctctc
      841 tgcgcgaagg gtattccgct ggtgaagccg ggaagcagga cgaagaccgc tccctgaacc
      901 acagcggaag gggctgattt actgtcgccc cgaacttccg gcagtcagca tcggagcttc
      961 ctctgacgcg attcggatgg tttttcaaga aacacgcccc cttgatgtgc cgggggcttt
     1021 tttctttgcc gtattcagtg ctacaataca cggtagtaag taattttacg taccggagcc
     1081 gtcgcagatt tcagggtggc cggctgtgtt gcgagtacgt tgttctggag gaagatcgcg
     1141 tgaaaaacac aactcgtctg ctcctggtgt caggactgct ctgcctgctc gtttttcttg
     1201 cgtacctttt tctcttctcc ggacaagaag ccccggagcc tctcgacggc gacccgttga
     1261 gggagcgggg gatgcgcgcg gcggcgatcg gcggcgaact cgtttcagtc gccaaagagc
     1321 agctcggcgc gcttcaggaa gagcacaggg cggatgtagg ggagctgctc ggcgaactgg
     1381 agcggaagac cgccgcagtg aaggagctcc tttcccgaaa ggacgccgat gaagatgatc
     1441 ttgccgccgc ggtgtccgca ctcgaaaaag cgctcgacgg cgtgctggtc gtccccgaca
     1501 acgaaacagg aaacgaggaa aggaggacgg attcatgaca tccgtaaacg aaacagccgt
     1561 tcttgcgcgg cgcgttaacg agatgctccg aaggtacatg aaggtgcagg aggatctatt
     1621 caaaccgtct ttgcgcaaga tactgcgtat accggggatc tacaggccga tcgactacgc
     1681 ggagaatctt cgcgcgctcg aagagctgct gcgggagctc gccgaagtca aggccgctat
     1741 ccggcgagaa gaacccgatg cggcgtctcc ggaggggaag tttctggggg tgcttcgggg
     1801 atatgtctcc ttgatgacct cggctgtcga aaagctggag aacatctgct cccgcctgaa
     1861 ggaacgaagc gagggggggg cgtacggcaa agatgaatac aagagcgaca tggcggcgct
     1921 gcgcgaaatt cagaaaaaac atcttgagtc cggcgtcgcg ctgaacgaaa tgatgaagac
     1981 gctctcccgc agcgacccgc ccaaagaaac gcaggacgac gaatcgaaga gctgaaaaca
     2041 aagcccccgg agaaacgctc cgagggtttt ttcattgcct tccttccctc tgccctgaaa
     2101 gcggcgcatc tgttttcccc cttcgtttct gcgggcactt tttttaaact ttattttatc
     2161 cttgagaccc tcttgacatt tccgttacac aaagggtatc ctatcaaatg tatgaagctt
     2221 gtattttgct tgtatacagg aaagcgtttc gagggggaga gcgccgtgcg ggagaagaag
     2281 aaagaccaag cctacgaaac aattcggacg ctggtactcc agcgcagatc ctccgattcc
     2341 ttcgtcatct ccgagaactc cctggccgcc gagctgaaca tgagcagaac gccgatacgg
     2401 gaagcgctgc agcgtctcca gatggagggg ttcatcgaga tccacccgca ccgcggcatc
     2461 gtcattcccg aggtttcggt aatcgaagtg aacgagacgt tcgctctccg gatggcgatc
     2521 gagg
//
LOCUS       DAOTIL010000363         2521 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminobacteriaceae bacterium isolate AMR_MDS_3026
            NODE_49022_length_2521_cov_2.935929, whole genome shotgun sequence.
ACCESSION   DAOTIL010000363 DAOTIL010000000
VERSION     DAOTIL010000363.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868050
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminobacteriaceae bacterium (wastewater metagenome)
  ORGANISM  Aminobacteriaceae bacterium
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae.
REFERENCE   1  (bases 1 to 2521)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2521)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:19:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,224
            CDSs (total)                      :: 3,198
            Genes (coding)                    :: 3,184
            CDSs (with protein)               :: 3,184
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2521
                     /organism="Aminobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_49022_length_2521_cov_2.935929"
                     /isolate="AMR_MDS_3026"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:3034609"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..715
                     /locus_tag="P5201_08080"
     CDS             <1..715
                     /locus_tag="P5201_08080"
                     /inference="COORDINATES: protein motif:HMM:NF012955.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HRV98531.1"
                     /translation="VGTHSNADHCGGNAFLKKRTGCLIAATPLEAAVMENTFMEPLLL
                     WTAFPFAEISNRFLQAKPSSVDILIRHSGVFDESGLEAVPLPGHFLEMIGVRTPDGVF
                     FVADALFSETLLEKYHIIFVLDVQAALETLTFLDKQEADWFVPSHAPAVEDIRPLAEA
                     NRRSLMRVSQAVLDSCSEPIGKDEIVKRVGALFGISMSITEFLLYSAAISAHLTWLRK
                     NALISPFVEDGLLLWRRTP"
     gene            complement(732..1658)
                     /locus_tag="P5201_08085"
     CDS             complement(732..1658)
                     /locus_tag="P5201_08085"
                     /inference="COORDINATES: protein motif:HMM:NF015509.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AEC family transporter"
                     /protein_id="HRV98532.1"
                     /translation="MNELLIIAPIAAVILLGRLLGTSGAVSPQTFRETNKILYWISIP
                     ALLLRLTVRANLDALGGWNLFFAVYGTYFLLPLIAFGIGRLFKEDRKRLAISALTSMR
                     SNQVFMGIPAVSIAMGNPGLEALSLFLAMSLVGYHVISIASSQLVITRELSVRSLLGT
                     ISKVVRNPMVCACLVGILCSSLGFREFPGWIDTTLKVLGDIGTGLALLALGASIKVVS
                     LKGVFRSTWRDCTIKLFLHPLTLFLFFSLLPVEKTMMQVVVFCSAMPVAVNSLVVAQG
                     MGMDEHYAAELIAVSTMLSVFTLPLWIRFLGI"
     gene            1809..>2521
                     /locus_tag="P5201_08090"
     CDS             1809..>2521
                     /locus_tag="P5201_08090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009164940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomannomutase"
                     /protein_id="HRV98533.1"
                     /translation="MHISPTIFREYDIRGIADVDLTDQVVSAIGQAFGTWLVRQGVRK
                     IVVGGDVRVSTRRISGALVEGLLRTGVDVLNIGTVTTPVLYWSLHHFEVGGGVMITGS
                     HNPKDMNGLKLAFRGATLYGDDIQEILRMVLEDDMADAAHPGSLNEADIRDEYVRMLL
                     SKCSLGPKKLKVVCDSGNGTAGMMIGRYLETLGCEVVSLFDTPDGTFPNHHPDPQQRE
                     NLVSLMDAVRREKADVGLA"
BASE COUNT          615 a          621 c          716 g          569 t
ORIGIN      
        1 cgtcgggaca cactcaaatg cggatcactg cggaggaaac gcctttctca aaaagcgaac
       61 gggctgtttg atagctgcca ccccattgga ggcggcggtt atggaaaata catttatgga
      121 gcctctgctg ctgtggactg catttccttt cgccgagata tcgaacagat ttctccaagc
      181 gaaaccatcc tctgtggata tacttatacg gcattccggg gtattcgacg agagcggact
      241 ggaagcagtt cctcttcccg ggcatttcct tgaaatgatc ggagtacgca cccctgacgg
      301 tgtgtttttt gttgcggatg ctcttttttc ggaaacgctt ctggagaagt atcatattat
      361 ttttgtgctc gacgtacagg ctgcgcttga gacgcttaca ttcctcgata agcaggaggc
      421 tgactggttc gttccgagtc atgcccctgc cgttgaggat attcgacctc tggccgaagc
      481 gaaccgccgg agcttgatga gggtgagtca ggcggttctt gacagctgct cggaaccaat
      541 agggaaggac gaaatagtca aaagggtagg cgctctcttc ggcatctcca tgtcgatcac
      601 ggagttcctt ctctacagcg cggcgatatc ggcgcacctg acctggttac gaaaaaacgc
      661 gctcatttct ccgtttgttg aggacgggct gctcttgtgg aggcgtaccc cctgacatgc
      721 ggcgtaatcc ctcagatccc taaaaaacgg atccacaagg gaagcgtgaa cacagagagc
      781 attgtcgaca cggcgatgag ctcggcggcg tagtgttcgt ccattcccat tccctgggcg
      841 actaccagag agttgacggc caccggcatt gcggagcaaa aaacaacgac ctgcatcatt
      901 gtcttttcga cggggaggag agagaaaaag aggaaaagag tcaagggatg aaggaagagc
      961 ttgattgtgc agtctctcca cgtggaacga aaaacccctt tgagggagac gactttgata
     1021 ctcgctccca gggcgagcag agcgagaccg gttccgatat cgccaaggac tttcagtgtg
     1081 gtatctatcc atccggggaa ctcacggaag ccgagggagg agcagagtat tccgacgagg
     1141 caggcacata ccatcgggtt tcgtacgact tttgatatcg ttccgaggag agaacgcaca
     1201 gaaagctccc tggtaataac aagttgggaa gaagcaatgg aaatgacatg ataccccacc
     1261 aggctcatgg ccaggaaaag cgacagcgct tcaagccccg ggtttcccat agcgattgat
     1321 actgcgggga ttcccatgaa aacctgattt gaacgcatgg atgtcagagc ggatattgca
     1381 agacgtttcc gatcctcctt aaataatctg ccgattccga aggctatgag tggtagcaaa
     1441 aagtacgttc cgtacaccgc gaagaaaaga ttccatcctc cgagagcgtc aaggttggcg
     1501 cggaccgtga gacggagcag aagcgccgga atagagatcc agtagagaat cttgttcgtt
     1561 tcccggaagg tctgcggaga aactgcgccg ctcgtaccga gcaatctccc gaggagaatc
     1621 actgctgcaa taggcgctat tatgaggagt tcattcacga ggagcacatc cttttctatg
     1681 caatacgcat accgaatctt gacttttagt ttgtataccc tgattataag ggtatacgca
     1741 cacgcgtttc gatgatacag cgagacgctg cggacgacaa gagcgacgtg tcaaaaggag
     1801 gaagtagaat gcatatttcg ccgactattt ttcgggagta cgatattcgc ggcatagcgg
     1861 atgtcgatct gaccgaccag gtagtctcag ctataggaca ggctttcgga acctggttgg
     1921 tccgtcaggg agtaagaaaa atcgtggttg ggggcgatgt ccgcgtttcc acccggagaa
     1981 tttcgggcgc tcttgtcgag gggctgctac gtacgggtgt ggacgttctc aatatcggta
     2041 ccgtcaccac gccggttctc tattggagcc ttcatcactt cgaggtcggc ggcggcgtca
     2101 tgattacagg cagccacaat cccaaggata tgaacggtct gaaacttgct tttagagggg
     2161 cgaccctgta cggagacgat atccaggaaa tcctccgtat ggtcctcgaa gacgacatgg
     2221 cggatgccgc ccatccgggt tctcttaacg aagccgacat tcgggatgag tacgttcgta
     2281 tgctgctgtc gaaatgctct cttggaccga aaaaactgaa ggtggtctgc gatagcggca
     2341 acggcactgc cggtatgatg ataggccggt acttggagac gcttggatgt gaagtcgtgt
     2401 cgctcttcga tacaccggac ggaacgttcc cgaatcatca ccccgatccg caacaacgcg
     2461 agaacctcgt ctccttgatg gatgctgtgc ggcgtgaaaa ggcggacgtc gggctcgctt
     2521 t
//
LOCUS       DAOTIL010000364         2519 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminobacteriaceae bacterium isolate AMR_MDS_3026
            NODE_49065_length_2519_cov_3.334821, whole genome shotgun sequence.
ACCESSION   DAOTIL010000364 DAOTIL010000000
VERSION     DAOTIL010000364.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868050
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminobacteriaceae bacterium (wastewater metagenome)
  ORGANISM  Aminobacteriaceae bacterium
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae.
REFERENCE   1  (bases 1 to 2519)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2519)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:19:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,224
            CDSs (total)                      :: 3,198
            Genes (coding)                    :: 3,184
            CDSs (with protein)               :: 3,184
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2519
                     /organism="Aminobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_49065_length_2519_cov_3.334821"
                     /isolate="AMR_MDS_3026"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:3034609"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            100..1590
                     /locus_tag="P5201_08095"
     CDS             100..1590
                     /locus_tag="P5201_08095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006301811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HRV98534.1"
                     /translation="MSIRLEEVIGKRLAGDQSQRVIWWKGTWWDAEKVEQKTEECASI
                     LREAGFQPGSRLAAFLPNSPLVLLLSMAAWRLGGTFAPLNPRGGVEWILRILDHLDPF
                     AVVVGEEMTPLEEAIRGAGIPVVAAPSDGVPPAFTGKRDRIGQDPDIAVIFATSGTTG
                     APKAVPLTHENLYDNTKGVHETIEGFDKGRVLMNVLPNFHSFGYTVCSVLPLLWGLPE
                     ALLPGFMPLKNLFEGLRDSGTGILIAVPTMLPFILGAASKGEKLPSELRYILTGGGKL
                     DPLLEKRFRDQLGVITYEGYGLTECSPVVSCNPSDALRKTGTVGPALPSYTVEVRGED
                     GHPVPPGQDGVLWLKGPSVAGGYFRDPELSAERFHDGWIDTGDMVRVDEDGYISILDR
                     VSDMIIVGGFNVYPQEVETVIKEIPGVKEAVVVGVSNPVSGEVPMAFVIKEETADLES
                     NTIISYCKEKMAHFKVPRKVKFVTELPLSAVGKVLKRKLREQMVRE"
     gene            1696..2175
                     /locus_tag="P5201_08100"
     CDS             1696..2175
                     /locus_tag="P5201_08100"
                     /inference="COORDINATES: protein motif:HMM:NF025701.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protease complex subunit PrcB family protein"
                     /protein_id="HRV98535.1"
                     /translation="MKMVFGVACAALLFLSLSSVDTVFACVACGDDDGAVLSFSKIAG
                     GSYGGVKERSFLVLSNEEEWKALWSRINGNVQPLPPLPEVNFSTHAVAAVFQGRKNSG
                     GYSIGVEAVIEMPDRVTVTVRECEPGPSSIVTSALSSPWEAVAFPLPEKPVLFTSVN"
     gene            complement(2268..>2519)
                     /locus_tag="P5201_08105"
     CDS             complement(2268..>2519)
                     /locus_tag="P5201_08105"
                     /inference="COORDINATES: protein motif:HMM:TIGR04564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SYNERG-CTERM sorting domain-containing protein"
                     /protein_id="HRV98536.1"
                     /translation="ALVASHFPNDGAATIRSRILNSVKKVDALRDKVSTGGRLDLYKA
                     LQTDSDIGDEDGVGGGCSLSSSPFALLLLLPLFVFRRRR"
BASE COUNT          517 a          693 c          776 g          533 t
ORIGIN      
        1 aagcctccgg cgaaacggag aaaaaagtga tatgcgtctg tttattctga agggagccct
       61 cgacagagtg cgccttcgcc gagacaggga gggttttgtg tgtccattcg acttgaagaa
      121 gtaatcggga aacgtcttgc cggggatcag tcgcagcggg tgatctggtg gaagggaaca
      181 tggtgggatg cggagaaggt tgagcaaaag accgaggagt gcgcgtccat actccgtgaa
      241 gcggggttcc aaccgggaag ccggcttgcg gcgtttcttc ccaacagtcc gctggttctc
      301 cttctctcca tggctgcctg gcggcttgga gggacttttg cgcccctgaa tccgcgcggc
      361 ggcgtcgagt ggattcttcg tattctcgat cacctggatc cgttcgcggt cgtcgtcgga
      421 gaggaaatga cgcctcttga agaagccatc cggggcgcag ggatacccgt cgtcgcggct
      481 ccctcggacg gcgttcctcc tgcgtttacc ggcaaacgcg acagaatcgg ccaagatccc
      541 gatattgcgg tgatttttgc tacctcgggg acgaccggcg cgccgaaagc ggtgccgctc
      601 acccatgaaa atctgtacga caacacgaag ggcgtccacg aaaccattga gggttttgac
      661 aagggacgcg tcctgatgaa cgtcctcccg aactttcact ccttcggcta cacggtgtgc
      721 agcgtgctcc cgctgctctg gggacttccg gaagccctgc tccccggctt catgccgttg
      781 aaaaacctct tcgaaggact gcgcgactcc gggaccggaa tcctgatcgc cgttcccacg
      841 atgcttccgt tcatcctggg cgccgcctca aagggggaga agctcccgtc cgagctgcgg
      901 tacatcctca ccggcggcgg aaagctcgat ccgctgctcg aaaaacgctt ccgcgaccag
      961 ctcggcgtca tcacctatga agggtacggg cttactgaat gctcccccgt ggtctcgtgc
     1021 aatccgagcg acgcgctccg gaagacggga acggtcggcc ccgcgctgcc gagctatact
     1081 gtggaagttc gaggggagga cggccatcct gtccctcccg ggcaggacgg cgtgctctgg
     1141 cttaaaggac cgtcggtggc cgggggctat ttccgcgatc ctgaactcag cgccgaacgc
     1201 ttccacgacg ggtggatcga caccggcgac atggtcaggg tggacgagga cgggtacatc
     1261 tccatcctcg acagggtctc cgacatgatt atcgtcggcg gcttcaacgt ctacccgcag
     1321 gaggtggaga cggtcatcaa agaaatcccc ggcgtgaaag aggccgtcgt ggtgggcgta
     1381 tcgaatcccg tcagcggcga agtgccgatg gccttcgtta taaaagaaga aaccgccgat
     1441 cttgagtcga atacgatcat ctcctactgc aaagagaaaa tggcgcattt caaggttccc
     1501 cgcaaggtga agttcgtgac ggagctccca ctgtccgcag tcggcaaggt gctcaagagg
     1561 aagctccggg agcagatggt acgcgaatag agcgccggat gacgaatacg ctcttttacc
     1621 catcagggaa acgctctggt ctgttattct ttcacaacag acttgtttga gctggagggg
     1681 tagcatgtga aacggatgaa gatggtgttt ggtgttgcgt gcgctgctct tttgtttctg
     1741 tctctctctt ctgtagatac tgtttttgca tgcgtagcct gcggagacga cgatggcgca
     1801 gttctctctt tcagcaagat cgccggagga agttacgggg gagtaaaaga acgttcattt
     1861 cttgtgcttt cgaacgagga agagtggaag gcgttgtgga gtagaatcaa cggcaacgta
     1921 cagccgttgc cgcctctccc cgaggtgaat ttttccacac atgcggtggc cgcggttttc
     1981 caaggcagga aaaacagcgg agggtactct atcggcgtag aagcggttat cgaaatgccg
     2041 gatcgcgtaa ccgtaacggt acgggagtgt gaacccggtc cgtcgagcat cgtgacgagc
     2101 gctctttcga gcccatggga ggcggttgcc ttccctttgc cagagaagcc cgttcttttt
     2161 acctcggtca actgacatcc cgacctgctt caaagaggac aagtaacaag agggcgtccc
     2221 gcagtttttc tggacgccct cttgttgtac attgtgacgc gtagcagcta ccttcttcgc
     2281 cgaaatacga acagcggcag aagcaacagc agtgcgaagg gggacgacga gagggagcag
     2341 cctcctccga caccgtcctc gtcgccaatg tccgaatcgg tctggagcgc tttgtagagg
     2401 tccaatcttc ctccggtgga cactttgtcg cggagcgcgt ccaccttttt gacgctgttc
     2461 aggatacggg agcggatcgt ggccgcaccg tcgttcggga agtgggaggc cacaagggc
//
LOCUS       DAOTIL010000365         2518 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminobacteriaceae bacterium isolate AMR_MDS_3026
            NODE_49097_length_2518_cov_2.496143, whole genome shotgun sequence.
ACCESSION   DAOTIL010000365 DAOTIL010000000
VERSION     DAOTIL010000365.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868050
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminobacteriaceae bacterium (wastewater metagenome)
  ORGANISM  Aminobacteriaceae bacterium
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae.
REFERENCE   1  (bases 1 to 2518)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2518)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:19:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,224
            CDSs (total)                      :: 3,198
            Genes (coding)                    :: 3,184
            CDSs (with protein)               :: 3,184
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2518
                     /organism="Aminobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_49097_length_2518_cov_2.496143"
                     /isolate="AMR_MDS_3026"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:3034609"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(131..1390)
                     /locus_tag="P5201_08110"
     CDS             complement(131..1390)
                     /locus_tag="P5201_08110"
                     /inference="COORDINATES: protein motif:HMM:NF017051.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRV98537.1"
                     /translation="MTRLVCISLDYTAANTAERASAWNEDRLRALKDEGRILEGLLLT
                     TCNRSEVYLRLPPDAETPSELTHPKARVYSGTELSLHLLRVLLGLESLACGESHIAAQ
                     VKACYAEGNDLCGPWLHRLFQSSLKMAKLLRTCYHPGMEPSIPRLMVDMLAPSSPPSP
                     LSVLVLGAGEMGEETSRLLLSLPLSGSPQREAKAATDGEAAPRAALKPAVGSLVLSNR
                     TPDRTEALFSRLLPRLERESGPKVATLPWEQWREASRRFDAIFFCTGSSVPLFSIEDA
                     RPGQQIFDLGSPPQVVRSAEVAGARLVAIDDLARTAEEATKRYRKKLCRLEEEATNAA
                     VSVMSELETLSGETYKRLVLHRAERIVADRAERTSKRTGTDEETLRRMGWSIVKALLS
                     PAFAESDPHARRLWKILAGELEESDDV"
     gene            complement(1387..>2518)
                     /locus_tag="P5201_08115"
     CDS             complement(1387..>2518)
                     /locus_tag="P5201_08115"
                     /inference="COORDINATES: protein motif:HMM:NF012799.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="cobalamin biosynthesis protein"
                     /protein_id="HRV98538.1"
                     /translation="LNEALRAQALLPEAIREIRTVEEKMDEPGLIELAASLDVPLLVV
                     PRKEILAMEETFSASAATKHLDLPGVAEPCAASAGKLLGRRIATEGVTVAFAIAEPRC
                     EPFEPAKRAEFDAAEGASPETGTKRGALFILGTGPGAGGSVTIDARQALEEADAVVGY
                     ALYVDLLPQEWLQGKVVERYSMGEEEKRVERAVSLAEEGNVVALLSGGDPVLFGLAGL
                     ALRTANGRVPAKVFPGVTAAQAAGALLGAPYVNGVALLSLSDYLQPWSAVLRALEGAA
                     LSGLTVALYNPVKRDLEEKLREVRRVFSAQGYETAYLVRDAGRNAPSVQKMPLAELDA
                     DRLDMRTLILLPGSSVEEFAGLLLDRRGYRREADEHKEESAQ"
BASE COUNT          409 a          826 c          865 g          418 t
ORIGIN      
        1 tcccgcgtcg agcagcgtcc ggattttgcg cagtccggtc gcgccgccgc cgaccacaag
       61 catcggtcct gcggaggcgt cgagacagat cataagggag gctcgctccc gggagcgccg
      121 cgacggagcg ttacacatcg tcggactcct ccagctcgcc cgcgagaatc ttccagagcc
      181 ttcgggcgtg ggggtccgac tcggcgaaag cgggggagag gagcgccttc acgatgctcc
      241 accccattct gcggagcgtc tcctcatcgg ttcccgttcg cttcgaggtc cgctcggccc
      301 tgtccgcgac gattcgttca gccctgtgga gaaccagtcg tttgtacgtc tcgcccgaga
      361 gcgtttcgag ctccgacatg accgagacag cggcgttcgt cgcctcctcc tccagtctgc
      421 aaagcttctt cctgtagcgc ttcgtcgcct cctccgccgt ccgggcgaga tcgtcgatgg
      481 cgacaaggcg ggcgccggcc acttccgcgg agcgcacgac ctgcgggggc gaaccgaggt
      541 cgaagatctg ctgtcccggt cgcgcatcct caatggagaa gaggggaacg gaggagccgg
      601 tgcagaagaa gatcgcgtcg aaacgcctcg acgcctcgcg ccactgttcc cagggcagcg
      661 tcgcaacctt tgggccggat tcccgctcaa ggcgcggcaa gaggcgggag aacaacgctt
      721 ccgtgcggtc gggagtccgg ttcgacagga cgagcgaacc gacagccggt ttgagggcgg
      781 cgcggggcgc ggcttcgccg tccgtcgcag ctttggcttc ccgttgcggc gagccggaga
      841 gcggcagtga aagaagcagc cgggaggttt cttctcccat ctcgcccgcc ccgaggacga
      901 ggacggaaag aggagaaggc ggggaggaag gggcgagcat atcgaccatc aggcgcggaa
      961 tcgagggctc catgcccgga tggtagcagg tccgcagcag ctttgccatc ttgagagagc
     1021 tttggaacag acgatggagc cacgggccgc agaggtcgtt tccttctgcg tagcacgcct
     1081 tgacctgcgc cgcgatatgc gactcgccgc aggcgaggct ctccagcccc aggaggacgc
     1141 gcagcaggtg gagggaaagc tccgtcccgg aatagacgcg agccttcggg tgcgtcagtt
     1201 cggacggcgt ctcggcgtcc ggcgggaggc gcaggtagac ctccgaacgg ttgcatgtgg
     1261 tcagcagaag tccctcaagg atccgcccct cgtctttcag ggcgcggagc cggtcttcgt
     1321 tccatgcaga agcgcgttcc gccgtgtttg ccgcggtgta gtcgaggctg atgcagacaa
     1381 gccgcgtcat tgcgcggact cctccttgtg ctcgtccgcc tccctgcgat agccgcgcct
     1441 gtcgaggaga agcccggcga actcctccac ggagcttccc ggaaggagga tcagcgtgcg
     1501 catgtcgagg cggtccgcat cgagttcggc gagcggcatc ttctgtacgg aaggagcgtt
     1561 ccgccccgcg tcgcggacca ggtacgcggt ttcgtacccc tgtgccgaga agacgcgacg
     1621 gacctcgcgg agcttctctt caagatcgcg cttcacgggg ttgtagaggg cgacggtgag
     1681 cccggagagg gccgctcctt cgagggcccg gaggactgcg ctccacggct ggaggtagtc
     1741 cgacagtgag agcagcgcga cgccgttgac gtagggagcg cccagcagcg cgccggcggc
     1801 ctgcgccgcg gtaacgccgg gaaagacttt cgcgggaacc ctgccgtttg ccgtgcgcag
     1861 cgcgagaccg gcgaggccga agaggacggg atcccccccg gagaggagcg cgacgacgtt
     1921 cccctcctcg gcgagcgaca ctgcgcgttc cacccgcttt tcctcctccc ccatggagta
     1981 gcgctccacg acctttccct gtaaccactc ctgcgggagc agatcgacgt agagggcgta
     2041 gccgacgacg gcgtccgctt cctccagcgc ctgtctggcg tcgatcgtga cggaaccgcc
     2101 cgctcccggg cctgtgccga gaatgaagag cgcgccgcgc ttcgtccccg tctccgggct
     2161 tgctccctcc gcagcgtcga attccgcgcg cttcgcaggt tcgaacggct cgcagcgcgg
     2221 ctcggcgatc gcgaaggcga cggtgacgcc ctcggtcgcg attctccgtc cgaggagctt
     2281 tcccgccgaa gcggcgcacg gttcggccac ccccggaaga tcgagatgtt tcgtcgccgc
     2341 ggaggcggaa aacgtctcct ccatggcgag gatttccttg cggggaacga cgagcagcgg
     2401 gacgtcgagc gatgcggcca gctcgatcag gccgggttcg tccatctttt cctcgacggt
     2461 gcggatctcc cggatcgcct cgggaagaag cgcctgggcg cgtagcgcct cgttgagg
//
LOCUS       DAOTIL010000366         2515 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminobacteriaceae bacterium isolate AMR_MDS_3026
            NODE_49186_length_2515_cov_3.194309, whole genome shotgun sequence.
ACCESSION   DAOTIL010000366 DAOTIL010000000
VERSION     DAOTIL010000366.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868050
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminobacteriaceae bacterium (wastewater metagenome)
  ORGANISM  Aminobacteriaceae bacterium
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae.
REFERENCE   1  (bases 1 to 2515)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2515)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:19:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,224
            CDSs (total)                      :: 3,198
            Genes (coding)                    :: 3,184
            CDSs (with protein)               :: 3,184
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2515
                     /organism="Aminobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_49186_length_2515_cov_3.194309"
                     /isolate="AMR_MDS_3026"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:3034609"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..742)
                     /locus_tag="P5201_08120"
     CDS             complement(<1..742)
                     /locus_tag="P5201_08120"
                     /inference="COORDINATES: protein motif:HMM:NF013048.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008417 - fucosyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006486 - protein glycosylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 10"
                     /protein_id="HRV98539.1"
                     /translation="MIVKLTTPSASWPLALQTPGATGMWDGVQFVIDRDCAHADAWVV
                     YEKLPGRMTTVCPPSRTILITGEPPMIKSYPEAFAAQFARVLTCHAMRHGGVISEQQA
                     LPWHYGREFDQSGNERFTESYDSLVRSDPFAAKSRLLSIICSTKRKRDGHRKRHEFVS
                     SLEQAPLPGLDIFGKGRAKEAACKRDAIAPYKYHIVLENSECADYWTEKLADAYLGGA
                     FPFYWGCPNIEKYFPQGAFARIDIERPEE"
     gene            complement(739..1353)
                     /locus_tag="P5201_08125"
     CDS             complement(739..1353)
                     /locus_tag="P5201_08125"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRV98540.1"
                     /translation="MFELHRRIREFAARIQWEMKGRPIPLPNALKQAILLEHARTHGI
                     RVFVETGTYEGKTLAAMVPYFDRLYSVELGEALYERACARFASVPKVRLFQGDSGKRL
                     EDILAIEREDRCLFWLDAHYSRGDTARGDEETPVCTELERIGRHPVKDHVILIDDARC
                     FGADSGYPDIPQVEEMARRLFPDSTFAVADDIIRIAPRTGNDGR"
     gene            complement(1367..2383)
                     /locus_tag="P5201_08130"
     CDS             complement(1367..2383)
                     /locus_tag="P5201_08130"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRV98541.1"
                     /translation="MKYKRLVRLILCSSLLVFAGQFLLFRASGVYDLALKSYLYDNAS
                     WVKEPPRVLLMGSSRCFHQLVPSVIAEQNGLKITDVVNAGQVAAGPFEMLHTYTQHID
                     MFGGVEFIFYVLDADFFFESLHIDKPYEKILLSRRQWEIIADEHGNFYHLPVVLFANA
                     VRGSPRKTGAEMGFEGLPHMDFLNIVMEDRSARYKNNLEHFPLSDFQLKSMVQLKEAF
                     EKNGGTLVFVLTPFWDIRLLGDDFFERTRIAARALDEALGPVRLIGSWKRDAYGLGYT
                     DFLDNSHLSLSGAKKLTASLFKNIASIRSASPIFLEERLFGKAGEYEETTGTRFHGHI
                     HEQR"
BASE COUNT          480 a          754 c          768 g          513 t
ORIGIN      
        1 cctcctcggg gcgttcgatg tcgatgcggg cgaacgcccc ctgcggaaag tacttttcga
       61 tgttgggaca cccccagtag aacggaaacg ccccgccgag gtaggcgtcc gccagctttt
      121 ccgtccagta gtcggcgcac tccgagtttt cgaggacgat gtggtacttg tagggcgcga
      181 tcgcgtcgcg tttgcacgcc gcttccttgg cccttccttt gccgaagatg tcgagccccg
      241 gaagaggcgc ttgctccagg gaggagacga actcgtgccg tttcctgtgc ccgtcgcgct
      301 tccgcttcgt cgagcagatg atggagagaa ggcggctctt cgctgcaaag ggatcgcttc
      361 ggacgaggga atcgtagctt tcggtgaacc gctcgttccc cgattggtcg aactcgcgcc
      421 cgtagtgcca cggaagcgcc tgctgttcgg aaataactcc tccatgccgc atcgcgtggc
      481 aggtgagcac cctggcgaac tgcgccgcga acgcctccgg gtagctcttg atcatcggcg
      541 gctcgccggt gatcagaatc gttcgcgacg gcgggcaaac cgtcgtcatc ctcccgggga
      601 gtttttcgta gacaacccac gcgtcggcgt gggcgcagtc ccggtcgatc acgaactgca
      661 ccccgtccca cattccggtt gctcccggcg tctgcagggc gagcggccac gacgccgagg
      721 gggtggtgag tttgacgatc atctgccgtc gttgccggtt cgaggagcga tgcggatgat
      781 gtcgtccgcg acggcgaagg tggaatccgg aaagagccgc cgggccatct cctcgacctg
      841 cgggatgtcc gggtatcccg agtccgcgcc gaagcatctg gcgtcgtcga tcagaatcac
      901 gtggtctttc acaggatgcc gtccgatgcg ttcgagctcc gtacagacgg gagtctcctc
      961 atctcctctc gccgtgtcac cgcgcgagta gtgcgcgtcg agccagaaga ggcagcgatc
     1021 ctccctctcg atcgcaagga tgtcctcaag cctcttgccg ctgtccccct ggaagaggcg
     1081 caccttgggt acggaggcga aacgagcgca ggcccgttca tacagcgctt ctcccagctc
     1141 gaccgagtag aggcggtcga aatacgggac catggcggcg agcgttttcc cctcgtaggt
     1201 ccccgtctct acgaacacgc ggatgccgtg cgttcgggcg tgctccagaa gtatcgcctg
     1261 tttgagggcg ttcggcaggg gaatcggcct gcccttcatt tcccattgaa tacgtgccgc
     1321 gaactcgcgg atgcgccgat gcaattcaaa cataccgaaa gtctccttat ctttgctcat
     1381 ggatgtgccc gtgaaatctc gtgcctgttg tctcttcgta ttccccggcc tttccgaaga
     1441 gacgctcctc caaaaagatc ggcgaagccg agcgtatcga tgcgatgttc ttgaaaagcg
     1501 aggcggtcag cttcttcgcg ccggagagcg acaggtgcga attgtccagg aagtccgtat
     1561 accccagccc gtaggcgtcc cgcttccagc ttcctatgag cctgacgggg ccaagcgctt
     1621 cgtcgagagc ccgggcagcg atgcgcgtcc gttcgaagaa atcgtcgccg agcagacgta
     1681 tgtcccaaaa aggcgtgagc acgaacacca gggttccgcc gttcttttcg aaggcttcct
     1741 ttaactgtac catacttttc agctggaaat cggagagggg aaagtgctcc aggttatttt
     1801 tatatcgggc gctcctgtcc tccatgacga tgttcagaaa gtccatgtgc ggcagcccct
     1861 cgaaccccat ctccgccccc gtcttgcgcg gcgatcctct gaccgcgttc gcgaagagga
     1921 caacgggaag gtgatagaag ttgccgtgtt cgtccgcgat gatctcccac tgccttctgc
     1981 tcagcagaat cttttcgtac ggcttgtcga tgtgcaagga ctcgaagaaa aagtcggcgt
     2041 cgaggacgta gaagatgaac tccacgccgc cgaacatgtc gatgtgttgt gtgtaggtgt
     2101 gcagcatctc gaacggcccc gccgcgacct gccccgcgtt gacgacgtcc gttattttca
     2161 gcccgttctg ctccgcgatg acggagggga cgagctggtg gaaacagcgg ctgcttccca
     2221 tcagcagcac gcgcgggggt tccttgaccc atgaagcgtt gtcgtacaga tatgatttca
     2281 gggccaggtc gtagacgccg gaagcgcgaa agagcaggaa ctgtccggcg aagaccagga
     2341 gagaggagca gagaataaga cggaccagac gtttgtattt catgggaacc ctctcttcta
     2401 gaattggaaa tagagaaact cggagacccg gttcaggaaa cagacgctaa tgagcagcac
     2461 gcctccgacc gcgacggccc agcgggcggt gaaggtcccc tcccggaagc gtgtg
//
LOCUS       DAOTIL010000367         2514 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminobacteriaceae bacterium isolate AMR_MDS_3026
            NODE_49201_length_2514_cov_5.262302, whole genome shotgun sequence.
ACCESSION   DAOTIL010000367 DAOTIL010000000
VERSION     DAOTIL010000367.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868050
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminobacteriaceae bacterium (wastewater metagenome)
  ORGANISM  Aminobacteriaceae bacterium
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae.
REFERENCE   1  (bases 1 to 2514)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2514)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:19:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,224
            CDSs (total)                      :: 3,198
            Genes (coding)                    :: 3,184
            CDSs (with protein)               :: 3,184
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2514
                     /organism="Aminobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_49201_length_2514_cov_5.262302"
                     /isolate="AMR_MDS_3026"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:3034609"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..328)
                     /locus_tag="P5201_08135"
     CDS             complement(<1..328)
                     /locus_tag="P5201_08135"
                     /inference="COORDINATES: protein motif:HMM:NF014862.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Clp protease N-terminal domain-containing
                     protein"
                     /protein_id="HRV98542.1"
                     /translation="MDFAKLTTKSREAFSDAQNKAVTYGHSEVDGEHLLLALAEQPEG
                     LIPRLLARMGVDTKSLVRKLEEELSRRVRVTGPGVEPGKVYVSQRLSRLLVKAEQRAE
                     QLKDEYI"
     gene            625..795
                     /locus_tag="P5201_08140"
     CDS             625..795
                     /locus_tag="P5201_08140"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRV98543.1"
                     /translation="MPRKSVRAEDIVYFPERILSKVSRVKDYPLTLIEAPSGFGKTTS
                     FREYLREHIPSS"
     gene            1048..2235
                     /locus_tag="P5201_08145"
     CDS             1048..2235
                     /locus_tag="P5201_08145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000499523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS110 family transposase"
                     /protein_id="HRV98544.1"
                     /translation="MLYLGIDIGKNNHEAGIVDGEGKQRGKSLRFANDQQGFFSLTSF
                     LEQKVGPGEELRVGMEATGHYWIPLYSFLHGKGFILHVINPIQSDSLRNFTIRGAKTD
                     SIDCFLIAHTIRFGDFSETRLADEDVLTLRNLARFRESLKDSCANYKRQVVTVLDQVF
                     PEYDALFSNMFGESSKAFLKTYGTPEQTMELDTKSLAALLRKASRGRHGTKKAEEIKA
                     LAGRSIGVPFCKDAFAFQLKILIEQIEFTEKQIGEIDKKLAQLLKKLNPVILSIPGVG
                     PATGAVILGEIGDINRFDKPKKLVAFAGIDPTVMQSGNFSGSHNRFSKRGSPYLRRAI
                     WLAAVVAARFDPVFKAFYEKKRGEGKAYGTAIGAVARKLAYTIFAILKTNKPYEVRLN
                     PEA"
BASE COUNT          594 a          699 c          620 g          601 t
ORIGIN      
        1 agatgtattc gtctttcagc tgttccgccc gctgctcggc cttcaccaaa agccgggaaa
       61 gccgctgcga aacgtacacc tttcccggct cgacgccagg ccctgtaacc cggacgcggc
      121 gcgaaagctc ttcctccagt ttgcggacga ggcttttcgt gtcgacgccc atgcgggcga
      181 gcaggcgcgg aataagccct tccggctgtt cggcgagcgc gagcagaaga tgctcgccgt
      241 ccacctccga atgcccgtag gttacggctt tattctgtgc gtccgagaaa gcttcccgtg
      301 actttgtcgt caatttagcg aaatccatcg cgatcactcc ttttgaccaa tactaaccaa
      361 tgggattata acgacattca gcgccggaag aaagagcaaa taaagcgtat atacccttga
      421 tatttcactt ttcctcttac aacctctttt ttccttttgt tttcgctgct tactgtggat
      481 tcaccatacc tcgcaggagc tgtattcact cctggagaca gaaaatacaa aatcattatc
      541 aaaactggat aaaatgcagt gtttagggta ccatagcaac cggaacctca ggccacccca
      601 ttacgatgca caggagagag tacaatgccc cgaaaaagcg tccgcgctga agatatcgtc
      661 tactttccgg aacggatttt gtcgaaagtc tcccgcgtca aggactatcc gctgacgctc
      721 atcgaagcgc cttccggatt cggcaagacg acctcgttcc gcgaatacct gcgcgaacac
      781 attccgtcct cctagaataa agtagttgca actgggatca gcaaatagcc cgtagagttg
      841 cgataatgtc agcatgcagg ccgagtgtcg ccggttgttt ccttggggtc tgaacctcgt
      901 tgaggagtct ggcgcctcgc ctcttgcggc tttgcacgga tgagctggat gttgcggccg
      961 ctcgcggtct tcttcgtctg tccaaggagg ttgtgcgccg tcggagtgcg aggatcccct
     1021 gcaaattcac tatacggagt gtgcgttatg ctgtatcttg gtatcgacat cgggaaaaac
     1081 aaccacgaag ccggaatcgt cgacggggaa gggaagcaac gagggaagtc cttgcggttc
     1141 gccaacgatc agcagggatt cttctctttg acgtccttcc ttgagcaaaa ggtcggacca
     1201 ggcgaggagc tgcgcgtcgg tatggaagct accggccatt actggattcc cctgtattcc
     1261 ttcctccacg ggaagggctt tatactccat gtcatcaacc ctattcagtc cgacagtctc
     1321 aggaacttta ccatacgagg cgcgaaaaca gactctatcg actgtttcct gatagcacac
     1381 accattcgct tcggggactt ctccgagacc cggttggccg acgaggatgt tctgacgctt
     1441 cgtaatctgg ctcgtttccg cgaatcgctc aaggacagct gcgcgaacta caaacgtcag
     1501 gtcgtcactg tactcgacca agtcttcccc gagtacgacg cgctcttctc caacatgttc
     1561 ggcgaaagct cgaaggcttt tctcaaaaca tacggaacac ccgagcaaac gatggaactc
     1621 gacacgaaat ctctcgccgc attgttgcga aaggccagca gagggcgtca cggcacgaaa
     1681 aaagcggagg agatcaaggc gcttgcggga cgctccatcg gagtcccgtt ctgcaaggac
     1741 gccttcgctt tccagctgaa gatccttatc gagcagatag agttcacgga aaagcagatc
     1801 ggcgagatcg acaagaagct cgcccaatta ctgaagaaac tgaatccggt cattctctcc
     1861 attccgggcg tcggccccgc cacgggcgcc gtcattctgg gcgagatcgg agacatcaac
     1921 cgctttgaca aacccaagaa gcttgtcgcc ttcgcgggaa tcgaccctac cgtgatgcag
     1981 tccggcaact tctcgggaag tcacaatcgc ttctccaaac gaggctcccc gtacctgagg
     2041 agagccatct ggttggccgc cgtcgtcgcc gcccgcttcg acccggtctt caaggcattc
     2101 tatgaaaaga aaagaggcga agggaaagct tatggtacag ccatcggcgc tgtcgccaga
     2161 aagcttgcgt acaccatttt cgcgatcctg aagacgaaca agccttatga agtccgactg
     2221 aatccggaag cataattccc aatctgcaat atgcttcgtg tttctccgat ctgttttccg
     2281 gtcggatcaa tttcgcatac tctttttcaa ggctatatta tgctgtcaag ttgcagtcat
     2341 gcatactgcc ttaaaatatc ctgccgacaa tctctttact ttttctcaac caaccctatt
     2401 gacttttgat agctggtctc ctcaagtggt gtcaggagta tcataataca cgcaaaattt
     2461 atattgaaga aatggagttt ttactgaaaa tgctttttcc tctttgcatt tggg
//
LOCUS       DAOTIL010000368         2501 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminobacteriaceae bacterium isolate AMR_MDS_3026
            NODE_49525_length_2501_cov_3.404742, whole genome shotgun sequence.
ACCESSION   DAOTIL010000368 DAOTIL010000000
VERSION     DAOTIL010000368.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868050
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminobacteriaceae bacterium (wastewater metagenome)
  ORGANISM  Aminobacteriaceae bacterium
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae.
REFERENCE   1  (bases 1 to 2501)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2501)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:19:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,224
            CDSs (total)                      :: 3,198
            Genes (coding)                    :: 3,184
            CDSs (with protein)               :: 3,184
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2501
                     /organism="Aminobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_49525_length_2501_cov_3.404742"
                     /isolate="AMR_MDS_3026"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:3034609"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..547
                     /locus_tag="P5201_08150"
     CDS             <1..547
                     /locus_tag="P5201_08150"
                     /inference="COORDINATES: protein motif:HMM:NF015366.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter
                     substrate-binding protein"
                     /protein_id="HRV98545.1"
                     /translation="IKTDPSAVPIGVGPTLGNNDHLMFLELAQIFGVDPASIKFIVYP
                     GAGGEIVPALLGGHVKATTIGLGEVLEQHKAGKMRIIGVSADQKLDFLPDVDTFTAQG
                     VNLVFPHWRGIIGAPDLTPEQVKYWDDVFTKMVQTETWKTLIANLGWSNFYQNSAEHT
                     TFLEKSTTDFDKLLTQVGLKK"
     gene            633..1106
                     /locus_tag="P5201_08155"
     CDS             633..1106
                     /locus_tag="P5201_08155"
                     /inference="COORDINATES: protein motif:HMM:NF018979.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter TctB
                     family protein"
                     /protein_id="HRV98546.1"
                     /translation="MSKNFMSGLLSLVLGAIYFFTATQFPEVTAGDRTGPKLFPLIIG
                     SVAMIAGGLLCVLDRRSGKGQPIEWNFVADRAVWSKILVIMLLGISYGLVLDSWGYLV
                     ATTVFMFCITLLINRRHFLQNLLISIFFPVVTYAAFAVALQLSLPRGIIENMLPF"
     gene            1120..>2501
                     /locus_tag="P5201_08160"
     CDS             1120..>2501
                     /locus_tag="P5201_08160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005660844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter permease"
                     /protein_id="HRV98547.1"
                     /translation="MDAIFQNLAIGFSTAITAYNILWVFIGGLLGTVMGMLPGLGPAT
                     GVAILIPITYGMNPSTALITMCAVYYGAMFGGSRASIMINTPGDASAIVSCFDGYPMT
                     KNGEAGAALAISAIASFIGGTIGMLFLVFLTGPIADAALRFGPAEMFSLMLFALVATI
                     TLAEGSLLKGVIAMCIGFMLSTIGIDALTGILRFTYGVEELQDGIDFLVVMIGVYAIC
                     EVFKNLKNLETKYNLDSKSIGKVWVSWPQFKKCIMPMLRSSPLGFFVGVLPGMGASIA
                     TFMSYAMEKNLSKTPEKFGKGAIEGLAAPEAANNSCAAGAMVPLLTLGIPGSGTTAVM
                     LGALMMLGVKPGPILFSQYPEIAWGVIASILIGNIILVIINLPLAIPLVQLLKLPQRV
                     MMPLILGMAFMGTYFLNYSSFDFILVMGFALGGYIFSKLEIPLPPMILALILGGTTEQ
                     SFRNSMSLSDG"
BASE COUNT          487 a          790 c          684 g          540 t
ORIGIN      
        1 tatcaagact gatccctccg ccgtaccgat cggcgttggc ccgacgctcg gtaacaacga
       61 ccacctgatg ttccttgaac tcgcacagat attcggcgtg gatcccgcaa gcatcaagtt
      121 catcgtctac cccggcgcgg gcggcgagat cgtcccggcg cttctcggcg gccacgtaaa
      181 ggccacgaca ataggcctcg gcgaagttct tgaacagcac aaagccggca aaatgcggat
      241 catcggcgtg agcgccgacc agaagctcga cttccttccg gacgtcgaca ccttcacagc
      301 gcagggcgtc aatctcgtct tcccgcactg gagaggcatc atcggcgctc ccgatctgac
      361 gccggagcag gtgaagtact gggacgacgt ctttacaaag atggtgcaga ccgagacctg
      421 gaaaaccctt atcgcgaatc tcgggtggag caacttctac cagaactccg ccgagcatac
      481 gaccttcctc gagaagagca caacggactt cgacaagctg ctgacgcagg ttgggctgaa
      541 gaagtagctc gtattccttt cggcggggag atcgacggat tttcccgccg tctttctttt
      601 ctgaagaaac aacagaaaac ggaggttgca gaatgagcaa aaacttcatg agcggactgc
      661 tctctctcgt cctgggagca atctattttt ttacggcgac gcagtttcct gaagttacgg
      721 caggagacag aacagggccg aagctcttcc ctctgatcat cgggagcgtc gccatgatcg
      781 ccggagggct tctctgcgtc ctggacagaa gaagcggaaa agggcaaccg atcgagtgga
      841 attttgtcgc ggaccgcgcg gtgtggtcga agattctcgt catcatgctg ctgggaatct
      901 cttacgggct tgtcctcgac tcttggggat accttgtcgc cacaacggtt ttcatgttct
      961 gcatcacgct gctcatcaac cggaggcact tcttgcagaa tcttctcatc tccatctttt
     1021 tccccgtggt cacatacgcg gccttcgcag tggctcttca gctgagcctc cctcgcggca
     1081 tcatcgagaa catgctcccc ttctaggagg tcggcacaca tggacgctat ctttcaaaat
     1141 ctcgcaatag gattctccac cgccatcacg gcctacaaca tactgtgggt cttcatcgga
     1201 ggccttctcg gcacagtcat gggcatgctc cccggcctcg ggcccgcaac cggcgtcgcc
     1261 atcctcatcc ccatcaccta cgggatgaac ccctcgacgg cgcttattac gatgtgcgcc
     1321 gtctactacg gcgcgatgtt cggcggctcc cgagcttcca tcatgatcaa cacgccgggc
     1381 gacgcctccg ccatcgtctc ctgcttcgac ggctacccga tgacgaagaa cggagaggcg
     1441 ggagcggcgc ttgccatctc ggccatcgcc tcgttcatcg gcggcacgat cggcatgctt
     1501 tttctggttt tcctcaccgg ccccatcgcg gacgccgcgc tccgtttcgg ccccgcggag
     1561 atgttctcgc tgatgctctt cgcgctcgtc gccaccatca cgctcgccga aggcagcctt
     1621 ctgaagggcg tcatcgcaat gtgcatcggc ttcatgcttt cgacaatcgg catcgacgcg
     1681 ctgaccggca tcctccgctt cacctacggc gtcgaggagc tgcaggacgg gatcgacttt
     1741 cttgtggtga tgatcggagt ctacgccatc tgcgaggtct tcaagaacct gaagaatctg
     1801 gagacgaagt acaacctcga ctcgaagagc atcggcaagg tgtgggtaag ctggccgcag
     1861 ttcaagaagt gcatcatgcc catgctccgc agctctcccc tcggcttctt cgtcggcgtg
     1921 ctcccgggaa tgggcgcgag catcgccacc ttcatgtcct acgctatgga gaagaacctg
     1981 agcaagacgc cggagaagtt cggcaagggg gccatcgaag gactcgcggc gcccgaggcg
     2041 gcgaacaact cctgcgcggc gggcgcgatg gtgccgctgc tcacgctcgg catccccggc
     2101 tccggaacga cggcggtcat gctcggcgcg ctgatgatgc tcggcgtcaa gcccgggccg
     2161 attctcttct cccagtatcc ggagatcgcg tggggcgtca tcgcctccat tctcatcggc
     2221 aacatcatcc tcgtcatcat caatctgccg ttggccattc ctcttgtgca gctcctgaag
     2281 cttccccaga gagttatgat gccgctcatc ctgggcatgg ccttcatggg aacgtacttt
     2341 ctgaattaca gctccttcga tttcatcctc gtcatggggt tcgccctcgg cgggtacatc
     2401 ttctccaaac tcgaaatacc gctgcctccg atgatactgg ctcttatcct gggaggcacc
     2461 acggaacagt cgttccggaa ctcgatgtcg ctctcggacg g
//
LOCUS       DAOTIL010000369         2499 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminobacteriaceae bacterium isolate AMR_MDS_3026
            NODE_49560_length_2499_cov_3.741408, whole genome shotgun sequence.
ACCESSION   DAOTIL010000369 DAOTIL010000000
VERSION     DAOTIL010000369.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868050
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminobacteriaceae bacterium (wastewater metagenome)
  ORGANISM  Aminobacteriaceae bacterium
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae.
REFERENCE   1  (bases 1 to 2499)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2499)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:19:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,224
            CDSs (total)                      :: 3,198
            Genes (coding)                    :: 3,184
            CDSs (with protein)               :: 3,184
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2499
                     /organism="Aminobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_49560_length_2499_cov_3.741408"
                     /isolate="AMR_MDS_3026"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:3034609"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..495)
                     /gene="rplB"
                     /locus_tag="P5201_08165"
     CDS             complement(<1..495)
                     /gene="rplB"
                     /locus_tag="P5201_08165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006583235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L2"
                     /protein_id="HRV98548.1"
                     /translation="MAIKKFNPYTPGRRFMTVQKNDDLTKKDPERSLLEPLHKKGGRN
                     NRGRITMRHRGGGAKRLYRVIDFKRNKLNVPGKVAAIEYDPNRNARIALIHYLDGDKR
                     YIIAPKDLNVGDKIVAGPESDIRPGNALKLKDIPVGTTVHNLELEPGRGAKLVRAAGT
                     SAQLM"
     gene            complement(517..813)
                     /gene="rplW"
                     /locus_tag="P5201_08170"
     CDS             complement(517..813)
                     /gene="rplW"
                     /locus_tag="P5201_08170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013048030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L23"
                     /protein_id="HRV98549.1"
                     /translation="MNLVAHDIIIRPLITEKSSRLMELNKYSFEVHLSANKIQVRKAI
                     EEIFKVKVTAVHILNIHPKPKRMGAFIGKTRSWKKAIVSLAPGERIDFFEGASI"
     gene            complement(810..1433)
                     /gene="rplD"
                     /locus_tag="P5201_08175"
     CDS             complement(810..1433)
                     /gene="rplD"
                     /locus_tag="P5201_08175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006583233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L4"
                     /protein_id="HRV98550.1"
                     /translation="MPTLKLVSFQGETVGEVQLSDGVFAAPVHVPAMHQVVVAQLANA
                     RLGTQSAKTRGEVRGGGKKPWRQKKTGRARHGSIRSPLWAGGGVTHAPKPRDYSQKVN
                     KKVRRLAIKSALSLKVREELMTVVKSFDIEKPSTKEMLAFLTAVKARKPLIILPEANE
                     AVLKSARNVPGAKVINMVNINVYDLLNAGTLILTPEVVEKLEEVYVG"
     gene            complement(1461..2087)
                     /gene="rplC"
                     /locus_tag="P5201_08180"
     CDS             complement(1461..2087)
                     /gene="rplC"
                     /locus_tag="P5201_08180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014806365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L3"
                     /protein_id="HRV98551.1"
                     /translation="MSIGILGKKLGMAQIYNEQGQATPVTIIQAGPCPVVDLKTPSKD
                     GYSALVLGFGEPNPRKLNKSRKGLFEKAEVEPKKTLREFRVDSVDEYAVGQQIDVTLF
                     TEGETINVSGTSKGKGFAGVVKKYHFAGSNASHGASVVHRRGGSSGASSYPGRVFKGK
                     KMPGRMGGEKVTVKNLVVVAVDSDNHLLLVKGAVPGAKNSLVTLFKKG"
     gene            complement(2121..2426)
                     /gene="rpsJ"
                     /locus_tag="P5201_08185"
     CDS             complement(2121..2426)
                     /gene="rpsJ"
                     /locus_tag="P5201_08185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014162799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S10"
                     /protein_id="HRV98552.1"
                     /translation="MTKKIRIRLKAFDHRVLDTSASQIADTAQRSGAKVSGPIPLPTE
                     INKYTILKSPHKDKDAREQFETRTHKRLIDIINPTQKTMDARMQLNLPSGVDIQIKL"
BASE COUNT          435 a          725 c          681 g          658 t
ORIGIN      
        1 catgagctgt gccgaagttc cggcggcacg gacgagtttc gctcctctgc cgggttcgag
       61 ctcaaggttg tgcacggtgg ttcccaccgg gatgtctttc agcttcagcg cgttgcccgg
      121 gcggatgtcc gactcggggc ccgcgacgat cttgtcgccg acgttcagat ccttgggggc
      181 gatgatatat cgtttatcgc cgtcaaggta gtgaatgagc gcgatcctcg cgttcctgtt
      241 cggatcgtac tcgatcgcgg caaccttccc gggaacgttg agcttgttcc tcttgaagtc
      301 gatgactctg tagagtctct tcgcgccgcc gcctctgtgg cgcatggtga ttctgccgcg
      361 attgttcctg ccgcccttct tgtgaagagg ctcgagaaga gagcgctctg gatccttttt
      421 ggtaagatcg tcgttcttct gaacggtcat gaacctgcgg cctggagtat aggggttgaa
      481 cttcttgatc gccattggtt tcctttctcc ttcccgctaa atgctcgcgc cttcgaagaa
      541 gtcgatacgc tctcccggag cgaggctgac tatcgctttt ttccatgaac gggtctttcc
      601 gataaaagcg cccattctct tcggcttcgg atggatattc aaaatatgaa cggccgttac
      661 tttgaccttg aagatctcct caatcgcctt gcgaacctgg atcttgttcg cggacaaatg
      721 gacttcaaag ctgtacttgt tcagctccat aaggcggctg ctcttttcgg tgatcagcgg
      781 acggatgatg atgtcgtgcg ctacgaggtt catccgacat acacctcctc aagcttttcc
      841 acgacttccg gggtcaggat cagcgtcccg gcgttgagaa gatcgtagac attgatgttc
      901 accatgttga tcaccttcgc gcccggtacg ttgcgggcgg acttcaatac ggcttcgttc
      961 gcctcgggaa gaatgatgag cggtttcctg gctttgaccg ccgtgaggaa cgcaagcatc
     1021 tccttcgtgg agggcttttc gatgtcgaac gatttaacga ccgtcataag ctcttcgcgg
     1081 actttcagcg acagcgcgct tttgatcgcg agacggcgta ctttcttgtt caccttctgg
     1141 ctgtaatccc tgggcttggg ggcgtgcgta acgccgccgc cggcccacag gggcgaacgg
     1201 atgctcccgt gacgggcgcg tcctgtcttc ttctgtctcc agggcttctt gccgccgccc
     1261 cggacttctc cccgcgtctt cgcggactgc gttccgagtc tcgcgttcgc aagctgggcg
     1321 acgactacct ggtgcatggc gggtacgtgc acgggggcgg cgaaaacgcc gtccgaaagt
     1381 tgcacttccc ctaccgtttc tccctggaag ctgacgagtt tcagggtagg catgttattt
     1441 ccggcctcct tctctgccga ctaccccttc ttgaaaaggg tcaccaggct gtttttcgcg
     1501 cccggtaccg cacccttgac aagaagcagg tgattgtcgg agtccactgc aacgacaacg
     1561 agatttttaa ctgtgacctt ctcgccgccc attctaccgg gcatcttttt gcccttgaaa
     1621 actcttccgg ggtagctgct tgcgccgctg gagccgccgc ggcggtgcac cacggacgcg
     1681 ccgtggcttg cgttcgaacc ggcgaaatga tacttcttca cgacgcccgc gaagccctta
     1741 cctttgctcg taccggagac gttgatcgtc tcgccctctg tgaagagagt gacgtcgatc
     1801 tgctggccga cggcgtactc gtcgacagag tcgacccgga actcgcgaag cgtcttcttc
     1861 ggctccactt cagccttctc gaacagaccc ttgcgggact tgttcagctt tctcggattg
     1921 ggctcgccaa atccaaggac gagtgcgctg tatccgtcct tcgagggcgt cttcaaatcc
     1981 accacggggc atggtccggc ctgtataatc gtcacaggcg tggcttggcc ctgctcgttg
     2041 taaatctggg ccattccgag ttttttcccc agaataccga tgctcattac actccaactc
     2101 ctttcagctc agagcttccc ctacaattta atctggatat ccacaccaga aggaaggttc
     2161 agctgcatac gcgcgtccat cgttttctgc gtcgggttga tgatgtcgat aaggcgctta
     2221 tgtgtccgtg tttcaaactg ttcgcgcgca tccttgtcct tatgagggga cttcagaatc
     2281 gtgtacttgt tgatctcagt gggaagcggt atgggtcctg agacctttgc cccgctcctc
     2341 tgggcagtat cagcgatctg ggatgcagaa gtgtcaagta cacgatgatc gaaggctttg
     2401 agccttatcc tgattttttt tgtcactgtc tttacccccc ggggttattc aaggatatcg
     2461 gtaaccacac ctgcgccaac ggtatgtccg ccttcacgt
//
LOCUS       DAOTIL010000370         2496 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminobacteriaceae bacterium isolate AMR_MDS_3026
            NODE_49637_length_2496_cov_3.364605, whole genome shotgun sequence.
ACCESSION   DAOTIL010000370 DAOTIL010000000
VERSION     DAOTIL010000370.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868050
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminobacteriaceae bacterium (wastewater metagenome)
  ORGANISM  Aminobacteriaceae bacterium
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae.
REFERENCE   1  (bases 1 to 2496)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2496)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:19:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,224
            CDSs (total)                      :: 3,198
            Genes (coding)                    :: 3,184
            CDSs (with protein)               :: 3,184
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2496
                     /organism="Aminobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_49637_length_2496_cov_3.364605"
                     /isolate="AMR_MDS_3026"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:3034609"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..713
                     /locus_tag="P5201_08190"
     CDS             <1..713
                     /locus_tag="P5201_08190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009200994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA];
                     GO_process: GO:0008652 - amino acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="Asd/ArgC dimerization domain-containing protein"
                     /protein_id="HRV98553.1"
                     /translation="EKWYGAAPRHPELLERAVYGLPELHRTELQGASLASGVGCNASC
                     SILGLYPLARAGILGDVRIEVRVGSSEAGATPTLGSHHPYRSRTLRVYEPFRHRHLAE
                     ILQELDLSEERVTMTMTAVEMIRGVQMIAQISLSRPVKEAELWKLYRSAYADEPFLSL
                     CPARPSHLRLPDPKLVTGSNRVLTGFSLHEDGTRLVVVCALDNLMKGAAGSAVQSANI
                     LLGLPETEGLNMLPVYPA"
     gene            744..1604
                     /locus_tag="P5201_08195"
     CDS             744..1604
                     /locus_tag="P5201_08195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014806846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="[LysW]-aminoadipate kinase"
                     /protein_id="HRV98554.1"
                     /translation="MNTLHSARTFRGVVKIGGARGNTFLPLLEELAARVKRGEQWALV
                     HGASSMMEDLSRAVGTEPLYVTSPGGFRSRFVGEKELAIFEAACCRFSVDLVSALGRL
                     GVRAVPLYPSSAKGASAKRKDSLRSVENGKVRMLRGNYSGSITSFDPSDVFAVWESGG
                     LPLLPPLAADEAAAGSNLNVDGDRMAAAAAAAVNADVLAILSNVPGLLRDPSDPSSKV
                     ESGTLSEWETIELFAQGNMKRKLLAAKEALEGGAETVLIADSRSAQPIEAGLSGGGTL
                     LCRGSMAAAG"
     gene            1574..>2496
                     /locus_tag="P5201_08200"
     CDS             1574..>2496
                     /locus_tag="P5201_08200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014806845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class III-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HRV98555.1"
                     /translation="MSGIYGSRGISIASGKGATVKDSEGREYLDFYCGSGAALFGHAH
                     PVLKQALVEAAELPWTIGLGMGSAVRDSFKARLNDILPDRSVFYCNSGTEAIEAALKL
                     ATSLRPGKKKVLALRRAFHGRTIGALSLTFNPQYRKEWAHLLPPVQHVKPEELPGAVD
                     ADTAVVFVEPVQGEGGVHPLAPSLGEAITEACRTTGALLVSDEIQSGWGRCGALSASP
                     LAGLEPDILCFAKGVAGGMPIGLMLWKKELGDFSASGHGSTYGGNPLSLAVASASLKL
                     LEDEGYPEKTRVNGDFFRKLLSGIDSPLVSEV"
BASE COUNT          416 a          827 c          840 g          413 t
ORIGIN      
        1 acgagaagtg gtacggcgcg gccccccgcc atccagagct tctggagcgc gcggtctacg
       61 ggcttccgga gctgcaccga acggagctgc aaggagcgtc cctcgcgagc ggcgtggggt
      121 gcaacgcctc ctgttccatc ctcggcctct accccctcgc ccgagcggga atccttgggg
      181 acgtgcgcat cgaggtgagg gtgggctcct ccgaggccgg cgcgacgccg acgctcggca
      241 gccaccaccc ctaccgcagc aggacgctcc gcgtctacga gccgttccgt caccgccatc
      301 tcgcggagat tctccaggag ctggatctct ccgaggagcg cgtcaccatg acgatgacgg
      361 cggtcgagat gatccgcggc gtccagatga tcgcccagat ttcgctctcc cgacctgtga
      421 aagaggcgga gctgtggaag ctgtacagaa gcgcctacgc ggacgagccg ttcctctcgc
      481 tctgcccggc gcgcccttcg cacctgcgcc ttccggaccc caagctggtc acgggaagca
      541 accgcgtcct caccggcttt tcgctgcacg aggacggcac gcggctcgtt gtcgtctgcg
      601 cgctggacaa tctgatgaag ggcgctgcgg gaagcgcggt ccagtcggcg aacattctgc
      661 tcggcctgcc cgaaaccgaa gggctcaaca tgctcccggt ctacccggcg tagcgcatca
      721 ctctatagaa aggagcttct gccatgaaca ctctgcattc ggcacggaca ttccggggcg
      781 tggtcaagat aggcggcgca cgcgggaata cattccttcc tcttctggag gagctggcgg
      841 cgcgcgtcaa gcgcggagag cagtgggcgc tcgtccacgg cgcaagctcc atgatggagg
      901 acctctcccg cgcggtcggg acggaacctc tttacgtcac cagcccgggc ggcttccgca
      961 gccgcttcgt cggcgagaag gagctggcca ttttcgaggc ggcctgctgc cgcttctcgg
     1021 tcgaccttgt gagcgcgctc ggccgtctcg gcgtccgcgc cgtccctctc tacccctcgt
     1081 cggcgaaggg agcgtcagcc aagcggaagg actctctccg gagcgtcgag aacggcaagg
     1141 tcaggatgct tcgcgggaac tacagcggct ccatcacgtc gttcgaccct tcggacgtct
     1201 tcgccgtctg ggagtcgggc ggtttgcccc tccttcctcc gctcgcggcg gacgaggccg
     1261 cagcgggatc gaacctcaac gtggacggcg accggatggc cgcggccgcg gctgcggcgg
     1321 tgaatgcgga cgtcctcgcc atcctcagca acgttccggg actgctccgc gatccctccg
     1381 acccgtcctc gaaggtggag agcggcacgc tctcagagtg ggagacgatc gaactcttcg
     1441 cgcaggggaa tatgaagcgg aagctgctgg ccgcgaaaga agccctcgaa gggggcgcgg
     1501 aaacggtgct catcgcggac agccgaagcg cgcaaccgat agaagccggt ctttcgggag
     1561 gaggtactct gctgtgtcgg ggatctatgg cagccgcggg ataagcatcg cgtccgggaa
     1621 gggcgcgacc gtcaaggaca gcgaaggtcg ggagtacctc gacttctact gcggcagcgg
     1681 ggcggcgctc ttcggccacg cgcatcctgt gctgaagcag gcgctcgtcg aggcggcgga
     1741 actgccgtgg accatcggcc ttggcatggg gtccgcagtg cgcgacagct tcaaggcgcg
     1801 cctgaacgac atcctcccgg accgttccgt cttctactgc aacagcggca cggaggccat
     1861 agaggccgcg ctgaaactcg cgacgtcgct ccgtccgggg aaaaagaagg tgcttgcgct
     1921 gcggcgcgcc ttccacgggc ggacgatcgg ggcgctctcg ctcacgttca acccgcagta
     1981 ccggaaggag tgggcgcatc ttctgccgcc cgtccagcac gtgaagccgg aagagctccc
     2041 cggcgcggtg gacgccgata cggccgtcgt cttcgtcgag ccggtacagg gcgaaggggg
     2101 cgtccatccg cttgccccgt cgctcgggga ggcgatcacg gaggcctgcc gcacaacggg
     2161 cgcgcttctt gtcagcgacg agatacagag cggctggggg cgctgcggcg ctctctccgc
     2221 gagtccgctc gccgggctgg aaccggatat tctctgcttc gcgaagggcg tcgcgggggg
     2281 gatgccgatc ggactcatgc tctggaagaa ggaactcggc gacttctccg cctccggaca
     2341 cggctccacg tacggcggca acccgctctc cctcgccgtg gcgagcgctt cgctgaagct
     2401 gctcgaggac gaaggctacc ctgaaaaaac gcgcgtcaac ggagactttt tcagaaagct
     2461 gctctccgga atcgactcgc cgttggtttc ggaagt
//
LOCUS       DAOTIL010000371         2479 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminobacteriaceae bacterium isolate AMR_MDS_3026
            NODE_50116_length_2479_cov_3.679455, whole genome shotgun sequence.
ACCESSION   DAOTIL010000371 DAOTIL010000000
VERSION     DAOTIL010000371.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868050
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminobacteriaceae bacterium (wastewater metagenome)
  ORGANISM  Aminobacteriaceae bacterium
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae.
REFERENCE   1  (bases 1 to 2479)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2479)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:19:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,224
            CDSs (total)                      :: 3,198
            Genes (coding)                    :: 3,184
            CDSs (with protein)               :: 3,184
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2479
                     /organism="Aminobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_50116_length_2479_cov_3.679455"
                     /isolate="AMR_MDS_3026"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:3034609"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..716
                     /locus_tag="P5201_08205"
     CDS             <1..716
                     /locus_tag="P5201_08205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008519757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter permease"
                     /protein_id="HRV98556.1"
                     /translation="TIVGMLPGAGATISSFISYNFAKQSAKDPETFGKGNPKGVAAAE
                     SANNGCVGGSLIPLLTLGIPGNSVAAAMMGGLMIQGLIPGPELFSRYGTLTYGFILSL
                     FIANIVFLVLGLYLAPYFARVTSTPNALLIPGIAILSVIGSYAINNNMFDVWLMIAFG
                     ITGYFLEKGGFSTGALVLGLILGPIAELGFGQSLIMSQGSPMIFFDRPLCILLWVITL
                     LLMIPAFSSSLKKTKNRTE"
     gene            1174..>2479
                     /gene="mgtE"
                     /locus_tag="P5201_08210"
     CDS             1174..>2479
                     /gene="mgtE"
                     /locus_tag="P5201_08210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009164184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015095 - magnesium ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015693 - magnesium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter"
                     /protein_id="HRV98557.1"
                     /translation="MDLQKKFLASVDKLFERKQFKSLKELLSTMEPADIAEILAEKAA
                     TERAFLFRLLPKDLAIEVFEFMEGTEREELLANFTDSEVASIIEEMSDDDRTALFDEL
                     PAKTVKKLLAHLSPEERKLANALLNYPEDSAGRIMTPEFIDLKEGMTVARALERIRAT
                     AAKKETIYTSFVVSPERRLKGTVHLEDLILAAPETLLTDIMDGQPVFVTTSTDQEEAA
                     KIMSRYDLQTLPVVDKEERLVGILTFDDILDVIQEEATEDFERMAGIQPVDENYLEAG
                     VFTLARKRLIWLLVCIATQLFSSYILQSYAFALESAVALAFFIPMLTGTGGNTGTQAA
                     TLVIRGFTLGEIIKSDIHKVLYKEMLSGILLGLALALLASLRAWTMGTGFGVALTVAI
                     SVVGVVMLGNLVGALLPFAAKKLKLDPAIMSGPFISTVVDILG"
BASE COUNT          514 a          766 c          671 g          528 t
ORIGIN      
        1 gcaccatcgt cggcatgctg cccggcgccg gggcgaccat ctcctcgttc atctcgtaca
       61 acttcgcgaa acagagcgcg aaggacccgg agacgttcgg aaaggggaac cccaagggag
      121 tggccgccgc ggaaagcgcc aacaacggtt gcgtcggagg ttcgctcatc ccgctgctca
      181 ctctcggcat tcccggaaac agcgttgccg ccgccatgat gggcggtctg atgatccagg
      241 ggctcatccc gggccccgag cttttttcac gctacggcac gctgacctac ggattcattc
      301 tttccctctt catcgcgaac atcgtcttcc tggtcctggg gctctacttg gcgccctact
      361 ttgcgcgcgt cacgtcgacg ccgaacgcgc tgctcattcc cggaatcgcc attctctctg
      421 tcatcggcag ctacgccatc aacaacaaca tgttcgacgt ctggctgatg atcgctttcg
      481 gcatcacagg atacttcctg gaaaagggcg gtttttccac gggcgcgctt gttctcgggc
      541 tcatcctcgg ccccatcgcg gagctcggat tcggccagtc gctcatcatg tcgcaaggtt
      601 ctccgatgat cttctttgac cgcccgctct gtatccttct ctgggtgatc accctgcttc
      661 tcatgatccc cgctttttcg agcagtctga aaaagacaaa aaacaggact gagtaaggaa
      721 ccgacccttc ggggtcggtt ttttactcca ctgcgcctgt gacgcacacg cgctcgcgag
      781 cgccgacaga agagcgcccg cgctgttttt gaatgctgca ttcgctcggg cgaagaaaag
      841 aaaacttgtc acaaatggaa gccagtccgg ctcgacattt tttccgtttt gtggtacttt
      901 tttagcatta ttattttccg ctgcctgaaa aggagatggt tcggtggata gcggagcgca
      961 acctttaagt agcactcttc tcgcgtagta cggcgccgca gcggccttcc tttccggtaa
     1021 gccgaagccc ggcgcccgcc gggattttca ttaccggaga tccctaacga aatgcactcg
     1081 gaacgaatac cgcatactcc gcttttcggg aaattgtctc cggaaggaga tgatgccgtg
     1141 ttgtgacgtt caggggacaa ggaggcgtcg tgtatggatc tgcagaaaaa attcctcgcc
     1201 agcgtggata aactctttga acggaaacag ttcaagagcc tcaaggagct cctgagcaca
     1261 atggaaccgg cggacatcgc cgagatctta gcggagaagg cggccaccga gcgcgcgttt
     1321 ctcttccggc ttctgccgaa ggacctcgcg atcgaggtct tcgagttcat ggagggaacc
     1381 gagcgcgaag agctgctcgc gaacttcacc gactcggaag tggcctcgat catcgaggag
     1441 atgtccgacg acgaccgcac cgcgctcttc gacgagctcc ccgcgaagac ggtaaaaaaa
     1501 ctgctcgcgc acctctcccc cgaggaacgg aagctggcca acgcgctgct gaactatccg
     1561 gaggactccg caggacgcat catgaccccg gagttcatcg acctgaagga aggcatgacc
     1621 gtcgccagag cgctggagcg tatccgcgcc acagccgcga agaaagagac catttacacg
     1681 tccttcgtag tgagccccga acgccgcctc aaggggacag tccacctcga agacctcatc
     1741 ctcgcagccc cggagacgct cctgaccgac atcatggacg gacagcccgt cttcgtcacc
     1801 acctcgaccg accaggaaga agccgccaaa atcatgtccc gctacgatct ccaaacactt
     1861 cccgtcgtcg ataaagagga acgcctggtc gggatactca ccttcgacga tatcctcgac
     1921 gttatccagg aagaagcgac cgaggacttc gagcgtatgg cgggtatcca gcccgtcgac
     1981 gaaaactacc ttgaagcggg cgtgttcacg ctggcgcgca agcggttgat atggctgctc
     2041 gtctgtatcg cgacgcagct tttttcttcc tatatcctgc aaagctacgc attcgctctt
     2101 gaaagcgcgg tggcgcttgc ttttttcatc ccgatgctga ccggcacagg aggaaatacg
     2161 ggcacgcagg ctgcaacgct cgtcattcgc ggtttcacgc tcggcgagat cattaagtcc
     2221 gatatacaca aagtccttta taaagagatg ctctctggaa tcctgctggg gcttgcgctt
     2281 gcactcttgg cctcactccg ggcgtggacc atgggaacag gtttcggcgt tgcgcttacc
     2341 gtcgccatct cggttgtcgg cgttgtgatg ctcggaaacc tcgtcggcgc gctcctccca
     2401 ttcgccgcaa aaaaactcaa gctcgaccct gcgatcatgt ccgggccgtt catcagcacc
     2461 gtcgtcgaca tcctcggcc
//
LOCUS       DAOTIL010000372         2478 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminobacteriaceae bacterium isolate AMR_MDS_3026
            NODE_50163_length_2478_cov_2.703673, whole genome shotgun sequence.
ACCESSION   DAOTIL010000372 DAOTIL010000000
VERSION     DAOTIL010000372.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868050
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminobacteriaceae bacterium (wastewater metagenome)
  ORGANISM  Aminobacteriaceae bacterium
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae.
REFERENCE   1  (bases 1 to 2478)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2478)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:19:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,224
            CDSs (total)                      :: 3,198
            Genes (coding)                    :: 3,184
            CDSs (with protein)               :: 3,184
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2478
                     /organism="Aminobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_50163_length_2478_cov_2.703673"
                     /isolate="AMR_MDS_3026"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:3034609"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..420
                     /locus_tag="P5201_08215"
     CDS             <1..420
                     /locus_tag="P5201_08215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006583284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015655 - alanine:sodium symporter
                     activity [Evidence IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA];
                     GO_process: GO:0032328 - alanine transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine:cation symporter family protein"
                     /protein_id="HRV98558.1"
                     /translation="SGVLTAQPELTGAQLSLSAFESVLGGTGTMILSVGLALFAFSTI
                     LGWYWYSETCGVYIFGTWISPLLKVLWVAVLVLGAAGGVFLGDKYGFLANLWDLSDTL
                     NGLMAVPNLVALLILSGELRRLVADFDKKRHSGELKI"
     gene            499..1611
                     /gene="alr"
                     /locus_tag="P5201_08220"
     CDS             499..1611
                     /gene="alr"
                     /locus_tag="P5201_08220"
                     /EC_number="5.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006301226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008784 - alanine racemase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine racemase"
                     /protein_id="HRV98559.1"
                     /translation="MHRATRMEVHLDNLKANFQAIRRHVGPGPEIFGVVKADAYGHGA
                     VPACKTLIEAGCRRFAVAIPDEALELREAGIDAPILVLGPTPERSVKEYVRKGITATV
                     TDLSFARALGAEAVRQNKQAAVHVKVDSGMGRIGFLPCDIPAVLDELLTISGIKIEGM
                     FTHFAVADEQRLDYTAYQYAGLQEALAVAAKRGINLSLRHACNSAGILACPDKYLDAV
                     RPGVILYGMWPSGECPQTIELKPTFEVKTEVALVKELPPRSGVGYGLRYMTRGVEEIA
                     VLPVGYGDGYSRALSMKIPVLIKGKRVPLSGNICMDQTMVNVTGMGVVTGDEVVLLGR
                     QGDEEITPEEMAAARNTINYEIPIAFLKRVPRIYVS"
     gene            1756..>2478
                     /locus_tag="P5201_08225"
     CDS             1756..>2478
                     /locus_tag="P5201_08225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014163428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016624 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, disulfide as
                     acceptor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent enzyme"
                     /protein_id="HRV98560.1"
                     /translation="MKTILPSLRNFPVEELTDSMVQSLEEAAAKCRINAVTMVTLPNS
                     GHPAGSLSSMEMYLTVYGVADSTPGNCSSFDRDYVAISHGHTSPGAYAALAAWGFFDP
                     MDAVAHFRQCGSPFQGHVERDVPGIDWGSVNLGQGLSAGVGFALAQRARGNGKRTYVL
                     MGDGGQTKGQSAEARRIASKEKLSNITALVDWNRIQISGSLESVMPADIPALWKADGW
                     DVLECDGHSFRELYAALKQASES"
BASE COUNT          510 a          700 c          725 g          543 t
ORIGIN      
        1 agcggcgtgt tgacagctca gcccgagctt accggcgcgc agctttccct ctccgccttc
       61 gagagcgtgc ttggcggtac aggaacgatg atactctctg tcggactggc tctttttgca
      121 ttctccacaa ttcttggctg gtactggtac agtgagacat gcggcgtcta tatcttcggc
      181 acttggatct ctccgttgct caaagtcctc tgggttgctg ttcttgtact tggagctgcc
      241 ggcggcgtat tccttggaga taaatacggt ttccttgcga acctttggga cctctccgat
      301 acgctcaacg gtctcatggc cgttccgaac cttgtggcgc tgctcatcct ctccggagag
      361 ctcaggagac ttgtggccga ctttgacaag aaacgccata gtggagaact caagatttaa
      421 gagctttgct gcttggcgta aataccggaa aggggcggcg tgccgcccct tcatttattt
      481 gaaaggagaa ggaactccat gcaccgtgcg actcgaatgg aagtgcatct tgacaacctg
      541 aaagcaaact ttcaggcaat tcgccgtcat gtcggccctg gcccggagat cttcggcgtc
      601 gtcaaggcgg acgcatacgg tcacggcgcc gttcccgcct gcaaaacgct tattgaggcc
      661 ggttgccgcc gtttcgcggt agccattccg gacgaagcgc tggagcttcg ggaagccggg
      721 atcgacgccc cgatactggt tctcggcccg actcccgagc gttcagtgaa ggagtacgtc
      781 cgcaaaggca taacagcgac cgttacggat ctctctttcg ccagggcgct tggggcggag
      841 gctgtccggc agaataagca ggccgctgtt cacgtgaaag tggacagcgg tatgggaagg
      901 atcggttttc tgccctgcga cattccggcc gtgctcgacg aactcctcac gatttccggt
      961 ataaagatag aaggaatgtt tacccacttt gctgttgccg atgagcagag gctcgactac
     1021 accgcgtatc agtacgccgg tcttcaggaa gcgctggccg ttgccgccaa gcggggaatc
     1081 aacctctctc tgcggcacgc ctgcaacagc gcaggtatac tcgcctgccc ggacaaatat
     1141 ctcgatgcgg ttcgcccggg tgttatcctc tatggcatgt ggccgtccgg cgagtgcccg
     1201 caaacgatcg aactgaagcc taccttcgaa gtgaagactg aagttgcatt agtcaaggag
     1261 cttccgccgc gaagcggcgt ggggtacggc ctgcgataca tgacgcgggg tgtcgaagag
     1321 atcgcggtgc ttcccgtagg gtacggcgac ggctactcgc gggctctctc catgaaaatt
     1381 ccagtactca taaaagggaa gcgcgtcccg ctcagcggca acatctgtat ggatcagaca
     1441 atggtcaacg tcactggaat gggcgttgtc acgggggacg aggttgttct tctcgggcgt
     1501 cagggagacg aagagattac gccggaggag atggcggccg ccagaaatac catcaactac
     1561 gaaataccga tagcattcct gaagagagtc cccaggatct acgtgtcctg acgaagacga
     1621 gggggcgctt ccgggaggga tcgtccccga tttctctttt ccaattacaa atgatcgttt
     1681 ccgccttcca tttttcacct cttttttccc ttcttgagta taattatcct gtcacaactc
     1741 aaggaggcga tctcaatgaa aacaattctt ccatctctga ggaacttccc cgtggaggaa
     1801 ctcaccgatt ccatggttca atctcttgaa gaggcggcgg cgaagtgccg catcaacgcc
     1861 gtcacaatgg taacgctgcc gaacagcgga caccctgcgg gctctctctc cagcatggag
     1921 atgtacctga ccgtgtacgg cgtcgccgac agcacgccgg gaaactgctc ctctttcgac
     1981 cgcgattacg ttgccataag ccacggccat acctctcccg gggcctacgc ggcgcttgcg
     2041 gcgtggggct ttttcgaccc catggacgca gtggcgcact tccgtcagtg cggctctccc
     2101 ttccaggggc atgttgagcg cgacgttccc ggaatcgact gggggagcgt aaacctcgga
     2161 caggggcttt ccgccggcgt cggtttcgct cttgcacaac gcgcgcgcgg aaacgggaag
     2221 cggacctacg tcctgatggg cgacggagga cagacaaaag gccagagtgc ggaggcgcgg
     2281 cggattgcct cgaaggagaa actatcgaat attacggcgt tggtggactg gaaccgcatc
     2341 caaatttcag gttccttgga atcggtgatg cccgccgata ttcccgctct atggaaagcc
     2401 gacgggtggg atgtgctgga gtgcgatgga cactctttcc gcgaacttta cgccgcactg
     2461 aagcaggcct cggaaagc
//
LOCUS       DAOTIL010000373         2476 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminobacteriaceae bacterium isolate AMR_MDS_3026
            NODE_50229_length_2476_cov_2.561751, whole genome shotgun sequence.
ACCESSION   DAOTIL010000373 DAOTIL010000000
VERSION     DAOTIL010000373.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868050
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminobacteriaceae bacterium (wastewater metagenome)
  ORGANISM  Aminobacteriaceae bacterium
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae.
REFERENCE   1  (bases 1 to 2476)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2476)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:19:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,224
            CDSs (total)                      :: 3,198
            Genes (coding)                    :: 3,184
            CDSs (with protein)               :: 3,184
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2476
                     /organism="Aminobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_50229_length_2476_cov_2.561751"
                     /isolate="AMR_MDS_3026"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:3034609"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..462
                     /locus_tag="P5201_08230"
     CDS             <1..462
                     /locus_tag="P5201_08230"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HRV98561.1"
                     /translation="LSGAPFPLAEGLTTTGEIPRETDFEGPGKRFFTVRDGEVVQDSL
                     PDDMGIVARVKGKGLVVLAGCSHSGIVNILKRVRALYPGEPVEGVVGGLHLVNGTEEK
                     MEKTLAGLKEFDPAWIACGHCTGFPMQAKLFQAFGKRFIPLTVGKRFIVGA"
     gene            complement(558..1334)
                     /locus_tag="P5201_08235"
     CDS             complement(558..1334)
                     /locus_tag="P5201_08235"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' exonuclease"
                     /protein_id="HRV98562.1"
                     /translation="MGNNSVFAVFDLETNGFKGASTVSASSIVFTAEGRIVDFFNRFY
                     HSEERPDHRTERVHGLTPERIAHFREEERYPSYFLEDWPSLAAFWERWNPEGIVVHNL
                     SFDLSFLPREAVRGRKWWCSMRGLTDFCKIPKNTDREGEWKWPRLSEAVRIARERLLE
                     PLPLAEAEESVGPALGHYGLSDCFELYGLFVRVLNQAPERVRFRAASALQLSPRRELY
                     PLPSAVRDSFVRERLDYSARIAEAAGDAERSRKLRSLGTD"
     gene            1456..2052
                     /locus_tag="P5201_08240"
     CDS             1456..2052
                     /locus_tag="P5201_08240"
                     /EC_number="3.2.2.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014806640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004844 - uracil DNA N-glycosylase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HRV98563.1"
                     /translation="MNDAPFNALRNEVGTCERCPLAETRTHAVFGEGPERTPVVLVGE
                     APGAKEDESGRPFVGLSGRLLSELLEEAGLPREKLFITNTVKCRPPENRLPSKAETAA
                     CRPFLAAQLRHIRPRLVITAGNAPTRALLGTKEGITTLRGRLHTCVWEGMELSVLPLF
                     HPSYLLRNRGRAEGSPIMLTLADIREALRLAREFTPGF"
     gene            complement(2036..>2476)
                     /locus_tag="P5201_08245"
     CDS             complement(2036..>2476)
                     /locus_tag="P5201_08245"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRV98564.1"
                     /translation="IRSLGLKVKLDTNGARPDILERILAEKLADYAAMDLKAPEETYS
                     LACGRDGDATLLPLVRKSIALLLRGAVTFEFRTTLVPGLHRIEDAPLLGRMAEGAPLF
                     VLQNFRPGNTLDPGFSSLSPFSMEFLEEFRTALLPFVGEVKIRA"
BASE COUNT          470 a          770 c          821 g          415 t
ORIGIN      
        1 ctctccggcg cgccgtttcc tttggccgag ggcctgacga cgacggggga aatcccgcgc
       61 gagaccgact tcgaggggcc ggggaagcga ttcttcacgg tacgcgacgg cgaggtggtc
      121 caggactccc tccccgacga catggggatt gtcgcccgcg tcaaagggaa ggggctcgtc
      181 gtcctcgccg ggtgcagcca ttccgggatt gtgaacatcc tgaagcgggt ccgcgcgctc
      241 taccccggcg agccggtcga aggcgtcgtc ggcggactgc acctcgtgaa cggaaccgaa
      301 gagaagatgg agaagacgct tgccgggctg aaagagttcg atccggcatg gatcgcctgc
      361 gggcactgca ccggttttcc catgcaggcg aagctcttcc aggcgttcgg gaaacgcttt
      421 attccgctca cggtggggaa gcgcttcatc gtcggcgcgt aagcggcgag cagctcccgg
      481 acgacagagg ggacggggcg ccagcttccc ttcatccgta gagaatacga cgccctgtcc
      541 tcttatggag cggtctctca gtcggttcca agcgagcgga gttttcggct ccgttcggca
      601 tctcccgccg cttcggcgat gcgcgccgaa tagtcgagac gctctctgac gaacgagtct
      661 cgaacggccg aaggcagagg gtagagctca cgccgggggg agagctgcaa cgcggaggca
      721 gcgcgaaagc gcacccgttc cggcgcttgg ttcaggactc tgacgaagag cccgtacagt
      781 tcgaagcagt cgctgaggcc gtagtgtccg agggccgggc ccacggattc ctccgcctcc
      841 gccagcggaa gcggttcgag caggcgctcc cgggcgatgc ggactgcctc ggagaggcgg
      901 ggccacttcc attcgccctc ccggtccgta ttcttgggga ttttgcagaa gtcggtgagt
      961 cctctcatcg aacaccacca ctttcttccc cgcaccgctt cgcgggggag gaaggagaga
     1021 tcgaaagaga ggttgtggac gacgattccc tccgggttcc atcgctccca gaacgcggcg
     1081 aggctcggcc agtcctccag gaaataggag gggtagcgtt cttcctcgcg aaagtgcgcg
     1141 atccgctccg gcgtgagccc gtgaactcgc tcggtccgat ggtcgggacg ctcctcggag
     1201 tgatagaaac ggttgaaaaa gtcgacgatc ctcccctccg cggtgaagac gatcgacgag
     1261 gcggagacgg tcgacgctcc cttgaagccg ttcgtctcca ggtcgaagac ggcgaagacg
     1321 ctgttgtttc ccatgaaata ccctccctgc acacgtttcg gacccctccg gggcgctcgc
     1381 ttttgaacgc tccggagacg ctgctattat gacagaacag ctcccgtaag ggaaagcgac
     1441 gaaaggacga aaagaatgaa cgacgcgcca ttcaacgcat tgagaaacga ggtcgggacc
     1501 tgcgaacgct gtcccctcgc cgagacgcgg acacacgccg tttttgggga aggcccggaa
     1561 agaacgccgg tcgtccttgt gggggaggcc cccggagcga aggaggacga atccggacgg
     1621 ccgttcgtcg gcctctcggg acgtttgctg agcgaactgt tggaggaggc cgggcttccc
     1681 agggagaagc tgttcatcac gaacaccgtg aagtgccgcc ctcccgaaaa ccgccttcct
     1741 tcaaaagcgg agacggccgc ctgccgcccg tttctcgctg cgcagctgcg gcacatcagg
     1801 ccgcgcctcg tcatcaccgc gggaaacgcg cccacccgcg cgctgctggg gacgaaagag
     1861 gggataacga cgctgcgcgg acgtcttcac acctgcgtct gggagggtat ggagctctcc
     1921 gttctgccgc tcttccaccc gagctacctc cttcggaacc ggggacgcgc cgaaggaagc
     1981 ccgatcatgc tcacgctggc cgacatccgt gaagcgctcc gcctggcgcg ggagttcacg
     2041 cccggatttt gacctctcca acgaagggga gaagcgcggt tcggaactcc tccagaaact
     2101 ccatggagaa gggggagagc gaggagaagc cgggatcgag cgtgttcccc ggacggaaat
     2161 tctgaaggac gaagagcggc gctccttcgg ccatacggcc gagaaggggc gcatcctcga
     2221 tacggtggag ccccggaacg agcgtcgtcc ggaactcgaa agtgacggcc ccgcgcagca
     2281 gaagggcgat gctcttccgc acgagcggaa ggagcgtcgc atcgccgtcc cggccgcagg
     2341 ccagggaata cgtttcctcc ggagccttga ggtccatagc cgcatagtcg gccagtttct
     2401 ccgcgaggat ccgctcgagg atgtccgggc gcgctccgtt ggtgtcgagc ttcaccttga
     2461 gcccgagcga tcgtat
//
LOCUS       DAOTIL010000374         2475 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminobacteriaceae bacterium isolate AMR_MDS_3026
            NODE_50251_length_2475_cov_3.257438, whole genome shotgun sequence.
ACCESSION   DAOTIL010000374 DAOTIL010000000
VERSION     DAOTIL010000374.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868050
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminobacteriaceae bacterium (wastewater metagenome)
  ORGANISM  Aminobacteriaceae bacterium
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae.
REFERENCE   1  (bases 1 to 2475)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2475)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:19:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,224
            CDSs (total)                      :: 3,198
            Genes (coding)                    :: 3,184
            CDSs (with protein)               :: 3,184
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2475
                     /organism="Aminobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_50251_length_2475_cov_3.257438"
                     /isolate="AMR_MDS_3026"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:3034609"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..508
                     /locus_tag="P5201_08250"
     CDS             <1..508
                     /locus_tag="P5201_08250"
                     /inference="COORDINATES: protein motif:HMM:NF013828.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HRV98565.1"
                     /translation="LLSTLATCSYIRERRDVLLVGATGAGKTYIACALGNAACRNYIT
                     VKYMRLPELLTDLSIGRGDGTIKKLLNHYKRKVSLLILDEWMLTPLSGLAAFDLLEIV
                     EARHQNASTIFISQCGPAGWHEHIDDGRVADAILDRIVHNAYEIVIDGEDSMRWRKSF
                     KRKPGTKG"
     gene            complement(574..1098)
                     /locus_tag="P5201_08255"
     CDS             complement(574..1098)
                     /locus_tag="P5201_08255"
                     /inference="COORDINATES: protein motif:HMM:NF012512.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal-phosphate dependent enzyme"
                     /protein_id="HRV98566.1"
                     /translation="MLDKALSSVLGAIGKTPLVRLDRIARAWNLEGTLYAKLEYLNPG
                     FSKKDRIALQMIEEAEESGALRPGQTVVELTSGNTGTGLAIVCGVKGYPFCAVMSKGN
                     SMERARMMRALGAEVVLVDQAPGSPPGQVSGEDLERVEERARTLVIITFRKSHLSPFA
                     EPPVSCFRATDICS"
     gene            complement(1181..2125)
                     /locus_tag="P5201_08260"
     CDS             complement(1181..2125)
                     /locus_tag="P5201_08260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006584253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HRV98567.1"
                     /translation="MKKILVTGANGQIGVELVVHLRKLYGAENVLATDLRKTPGAASE
                     GGPFATLDVRDPKAFSETLRSFGADTVLHLAGILSARGEADPMFSWDVNVNGTCAALE
                     AAREHGAAFFFPSSIAAFGPSTPSKNTPQDTLQRPSTVYGVAKVSMELLCDYYHKKFG
                     MDTRGLRFPGLISYEALPGGGTTDYAVHIYYDAIKKGSYTSFIAQGTYMDMMYMPDAL
                     NAIVRLMEADPARLLHRNAFNISAMSFEPEQIAASIRKFLPGFTMDYDVDPLRQGIAE
                     SWPDSLDCSAAREEWGFAPEYDLDRMSEDMLARLKEKL"
     gene            complement(2122..>2475)
                     /locus_tag="P5201_08265"
     CDS             complement(2122..>2475)
                     /locus_tag="P5201_08265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006300011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HRV98568.1"
                     /translation="VAANLASVEILQQSEDLVKKLWHNGDFLKRELKALGFDTAHSET
                     PITPVIIGEASDAKAFSAKLFEAGVFATAIVFPTVPRGTARIRAMVSAAHSEDDLRFA
                     VEKFAAVGKAMGIIK"
BASE COUNT          458 a          838 c          733 g          446 t
ORIGIN      
        1 gcttctctcc acgcttgcga cctgctcgta tataagagag cgccgagatg ttcttctggt
       61 cggcgctacg ggcgcaggaa agacctatat tgcttgtgct ctcggcaacg ccgcatgccg
      121 gaattatatc accgtcaagt acatgcggct ccccgaactg ctcacggacc tgtcgatagg
      181 cagaggggac ggcacaatta aaaagctgct gaatcactac aagaggaagg tctcgctcct
      241 catactggac gaatggatgc tgacgccgct gagcggcttg gccgcgttcg atctgctgga
      301 aatagtcgaa gccagacacc agaacgcgtc gacgatcttc atctcgcagt gcggaccggc
      361 cggatggcac gagcatatcg acgatgggag agtggccgac gccattctgg acaggatcgt
      421 ccacaacgcc tacgagatcg tgatcgacgg cgaggactcc atgaggtgga gaaagagctt
      481 caaacggaaa ccaggaacca aggggtaact cccttgtgaa cggcgaagcc ccgcaatggc
      541 ggcttcgccg ccacaagacc ggaaagggtg gctctaagaa cagatatccg tggctctaaa
      601 acaggataca ggtggctctg caaatggaga caagtggctc tttcggaacg tgataatcac
      661 gagcgttcga gcacgctcct ccacacgctc cagatcctct ccggagacct gccccggagg
      721 cgaccccggc gcctggtcca ccagtacgac ctccgccccc agagcgcgca tcatccgggc
      781 gcgctccatc gaattcccct tcgacatcac cgcgcagaag gggtacccct tcacgccgca
      841 gacgatggcc agccccgtcc ccgtattgcc gctcgtcagc tccaccacgg tctgccccgg
      901 acggagcgcg ccgctctcct ccgcctcctc gatcatctgc agggcgatgc ggtccttctt
      961 gctgaacccc ggattcagat actcaagctt cgcgtagagc gtcccctcca gattccacgc
     1021 cctcgcgatg cggtccagac gcaccagcgg cgtcttccct atggctccca gaacgctcga
     1081 gagcgctttg tcgagcatgt tgcgttcccc ctctcgttgt gcccggccgc agagccggaa
     1141 tacaaaaaac cccccggttg gagtaccggg gggcgtgcgc tcaaagcttt tccttcagcc
     1201 gcgcaagcat atcctcgctc atccggtcga ggtcgtactc cggggcgaag ccccactctt
     1261 cgcgggcagc cgagcagtcc agcgaatcgg gccacgactc ggcaatcccc tgccgcagcg
     1321 ggtccacgtc gtagtccatc gtaaaaccgg gcaggaactt ccggatggag gccgcgatct
     1381 gctccggctc gaagctcatc gccgagatgt tgaaggcgtt ccggtgcaga agtctcgccg
     1441 ggtcggcctc catcaggcgg acgatggcgt tcagcgcgtc cggcatgtac atcatgtcca
     1501 tgtacgtccc ctgcgcgatg aagctggtgt agctcccctt cttgatcgcg tcgtagtaga
     1561 tgtgcacggc gtagtccgtc gtgccgccgc cggggagcgc ctcgtagctg atcaggccgg
     1621 ggaagcgcag tccgcgcgtg tccatgccga acttcttgtg gtagtagtcg cacaggagct
     1681 ccatgctcac cttcgctacg ccgtagaccg tcgagggccg ctggagcgtg tcctgcggcg
     1741 tgttcttcga gggcgtcgac gggccgaacg ccgcgatcga gctggggaag aagaaggccg
     1801 cgccgtgttc gcgggccgcc tcaagcgccg cgcacgtgcc gttcacgttc acgtcccacg
     1861 agaacatcgg gtccgcctcc ccccgcgccg aaaggatccc cgcaaggtgg agcaccgtgt
     1921 ccgcgccgaa cgagcgcagc gtctcggaga acgctttcgg gtcgcgcacg tcgagcgtcg
     1981 cgaacggacc gccctccgag gccgcgccgg gcgtcttccg aaggtcggtc gccagcacgt
     2041 tttctgctcc gtagagcttc cgcagatgga cgaccagctc cacgccgatc tgtccgttgg
     2101 cccccgtcac aagaatcttc ttcattttat gatccccatc gccttgccca ctgcggcgaa
     2161 cttctccaca gcgaagcgca ggtcgtcctc ggagtgcgcc gccgagacca tcgcccggat
     2221 gcgcgccgtg ccgcgcggca ccgtcgggaa gacgatcgcc gtggcgaaca cgcccgcctc
     2281 gaagagcttc gcgctgaacg ccttcgcgtc cgaggcctcg ccgatgatca ccggcgtgat
     2341 cggcgtctcg ctgtgcgccg tgtcgaagcc aagcgccttc agctcgcgct tcaggaagtc
     2401 gccgttgtgc cacagcttct tcacaaggtc ctcgctctgc tgcaaaatct ccaccgaggc
     2461 caggttcgcc gccac
//
LOCUS       DAOTIL010000375         2465 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminobacteriaceae bacterium isolate AMR_MDS_3026
            NODE_50500_length_2465_cov_2.780083, whole genome shotgun sequence.
ACCESSION   DAOTIL010000375 DAOTIL010000000
VERSION     DAOTIL010000375.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868050
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminobacteriaceae bacterium (wastewater metagenome)
  ORGANISM  Aminobacteriaceae bacterium
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae.
REFERENCE   1  (bases 1 to 2465)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2465)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:19:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,224
            CDSs (total)                      :: 3,198
            Genes (coding)                    :: 3,184
            CDSs (with protein)               :: 3,184
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2465
                     /organism="Aminobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_50500_length_2465_cov_2.780083"
                     /isolate="AMR_MDS_3026"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:3034609"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..494
                     /gene="tsaA"
                     /locus_tag="P5201_08270"
     CDS             <1..494
                     /gene="tsaA"
                     /locus_tag="P5201_08270"
                     /inference="COORDINATES: protein motif:HMM:TIGR00104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="tRNA
                     (N6-threonylcarbamoyladenosine(37)-N6)-methyltransferase
                     TrmO"
                     /protein_id="HRV98569.1"
                     /translation="NEDRARCVHPPGDKGKEGERMNEEKIQFFQIGVVRSSLLTRADC
                     PKQGVEGGVEAEIFVAPEYREGLDGLEPGRDVLLLTWLHRAERDVLKVHPRGNREAPL
                     KGVFATRSPARPNPVGLHRVKILELEKERGRLLVAPLEALDGTPVLDIKPVLRESGPF
                     SEA"
     gene            639..>2465
                     /locus_tag="P5201_08275"
     CDS             639..>2465
                     /locus_tag="P5201_08275"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRV98570.1"
                     /translation="MGVPSAAERTVSSTLRRNAVILCMVLFGVLFSWLVVERTDRKMR
                     DDLLYQTRLGVQTIDLDQVRALSGADEDVGKEEYHSLRRRLSAFREAADCRYVYLMQR
                     RPVRDNPGATEVVFLVEAQNDGIETSPPSQPGDVYEEEYITEEFAALFDGGAPFVEGP
                     VEDEWGIWISALVPMVDHRTGKLLAVLGVDYDAKLWRWTVVSSSLLPVSLMLVLLLIC
                     IALFVLSRPVDPMPRPVLKRLLPTMAVALLLLTSAAVLLLWRTYSDNLDERLNSLHAS
                     ISAEFHLDLKNELSLMESSIASLAEAPRLQWALFAGDADALFSDWLSMFARLRREYGV
                     RSFSFANEEFRRLYSFAPGSDEKLFYDGPVVLKAKETGKIASGVEVCPSGNLVLRAVL
                     PVSRAGELVGYAIITKDVQGLLQKRSLRSGSHLALSLRKGLLDRAKWEKTCNPDRTTE
                     WEQFPDCVIVFSSMERLPPGIFSSGEDDAPDTFRERRRESVFGEKIWGVSVLSFEDVS
                     GESVGEFAIMTDITADRNHLRSQVAMMWGIGGTIAAALLILLFTLLRRVDAGILAQQK
                     ALRESDALLKKLSRQIPGVLYQYRQEPGEQIDLLFISENVTDV"
BASE COUNT          479 a          642 c          797 g          547 t
ORIGIN      
        1 acaatgaaga ccgggcgagg tgcgtgcatc ctcccgggga taaaggaaaa gagggagaga
       61 ggatgaacga ggagaagata cagttcttcc agataggtgt ggtgcgctct tctttgctga
      121 cgcgggcgga ctgtccgaag cagggggttg agggtggcgt ggaggcggag atctttgttg
      181 cgcctgaata cagggagggg ctcgacggtc tggagccggg gcgggatgtg ctgctcttga
      241 cgtggctgca tcgggcggag cgggacgtcc tcaaggtcca cccgcgcggg aacagggaag
      301 cgcctctgaa gggcgtcttc gcgacccgtt ctccggcgcg tccgaatccg gtagggctgc
      361 acagagtgaa gatccttgaa cttgagaagg agaggggccg tcttctggtg gcgcctctgg
      421 aggcgctcga cggcacgccg gttctggata ttaaacccgt gctccgggag tccggaccat
      481 tcagcgaagc gtagatattt ttcaggaagt aagaatgaca tggaagaaaa gctgatatat
      541 atagtgtaaa atagatgtgt tcgcaaaggt tcggtactcg atagacgcaa cggaaaaagc
      601 cgatgatttc ggagttttcc cccgaagggg agatgtgtgt gggagttccc tctgctgcag
      661 aaagaacagt gtcttcgacg ctccgccgga acgcggtcat cctgtgtatg gtcctcttcg
      721 gagtgttgtt ttcatggctg gtcgtcgagc ggacggacag gaagatgcgg gacgatctgc
      781 tctatcagac gcgccttggt gtgcagacga tagaccttga ccaggttcgc gcgctctccg
      841 gcgccgatga ggacgtggga aaagaggagt atcacagcct gcggcgccgt ctttcggcat
      901 tccgggaggc tgcggattgc cgctacgtct acctgatgca acgcaggccg gttagggaca
      961 atccgggggc gacggaggtc gtgtttcttg tcgaggcgca gaacgacggg atcgaaacat
     1021 cccctccgtc gcaaccagga gacgtctacg aggaagaata tatcaccgag gagttcgccg
     1081 ctctcttcga cggaggcgcg cctttcgtcg aaggcccggt ggaggacgag tggggtatct
     1141 ggatttctgc cctcgtcccc atggtggacc atcggacggg gaagctgctc gccgtcctcg
     1201 gggtggatta cgacgcgaag ctctggagat ggaccgtcgt ctcgtcttcg ttgctgccgg
     1261 tatccttgat gctggtgctt ctgttgattt gtatcgcgct gttcgtcctc tcccggcctg
     1321 tggatcccat gccgcgtccg gtgctgaaga gactgctgcc gaccatggcg gtcgccctgc
     1381 ttctcctcac ctccgcagcg gtgcttcttc tgtggcgtac gtattccgac aatcttgacg
     1441 agcgactgaa ctcccttcat gcgtcgattt ccgccgaatt tcatctggac ttgaagaacg
     1501 aactctccct tatggagtcc tcgattgcct ctcttgcgga ggccccccgt ctgcaatggg
     1561 cgctcttcgc cggcgacgcg gacgcgctct tctccgactg gctgtcgatg ttcgcccgcc
     1621 tgcgccggga atacggcgta cgctcttttt cctttgccaa tgaggagttc cggcgtctct
     1681 attcctttgc gccggggagc gatgaaaaac tgttctatga cgggcctgtc gtgctgaagg
     1741 cgaaggaaac gggaaagatc gcctccggtg tcgaggtctg tccttccgga aatctggtgc
     1801 tgcgggccgt cttgccggtt tcaagggctg gagagcttgt ggggtatgcc attataacca
     1861 aagatgttca aggtctcctc caaaaaagat cccttcgttc cggatcccac ctggcgcttt
     1921 cactcagaaa aggcctactg gacagggcga agtgggaaaa aacgtgtaac cccgaccgga
     1981 caacggagtg ggagcagttc cccgactgtg tgattgtctt ttcatcaatg gagcgccttc
     2041 ctcccggtat cttttcttcc ggagaagacg atgcgcctga tacttttcgg gaacgaagac
     2101 gggagtcggt cttcggtgaa aaaatatggg gagtctccgt cctttcgttc gaggacgtct
     2161 cgggagagag cgtcggagag tttgcgatca tgacggatat cacggcggac aggaaccatc
     2221 ttcggagcca ggtcgcgatg atgtggggca tcggcgggac gatcgcggcg gcgctgctca
     2281 ttcttctctt cacgctgctg aggcgggtgg atgccggaat ccttgcgcag caaaaggcgc
     2341 tccgggagag cgacgcgctt ttgaagaagc tgtcgaggca gatcccgggg gtgctctatc
     2401 agtaccgcca agagcccgga gagcaaatcg atctgctctt catatcggaa aacgtcacgg
     2461 atgta
//
LOCUS       DAOTIL010000376         2459 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminobacteriaceae bacterium isolate AMR_MDS_3026
            NODE_50667_length_2459_cov_2.919717, whole genome shotgun sequence.
ACCESSION   DAOTIL010000376 DAOTIL010000000
VERSION     DAOTIL010000376.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868050
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminobacteriaceae bacterium (wastewater metagenome)
  ORGANISM  Aminobacteriaceae bacterium
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae.
REFERENCE   1  (bases 1 to 2459)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2459)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:19:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,224
            CDSs (total)                      :: 3,198
            Genes (coding)                    :: 3,184
            CDSs (with protein)               :: 3,184
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2459
                     /organism="Aminobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_50667_length_2459_cov_2.919717"
                     /isolate="AMR_MDS_3026"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:3034609"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..720)
                     /gene="gmd"
                     /locus_tag="P5201_08280"
     CDS             complement(<1..720)
                     /gene="gmd"
                     /locus_tag="P5201_08280"
                     /EC_number="4.2.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006301436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008446 - GDP-mannose 4,6-dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0000271 - polysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-mannose 4,6-dehydratase"
                     /protein_id="HRV98571.1"
                     /translation="MKKKALLTGITGQDGAYLAEFLLNKGYEVHGMKRRSSLFNTGRI
                     DHLYEDPHQTERRLILHYGDLTDSSNIIRIIQEVQPDEIYNLAAQSHVKVSFETPEYT
                     ANGDGLGVLRILEAIRILGLEKRTRFYQASTSELYGKVQEIPQNEKTPFYPRSPYAAA
                     KLYGYWITVNYREAYGMYACNGILFNHESPLRGETFVTRKITRAAARIALGLQEKLHL
                     GNLDAKRDWGHARDYVEAMWLM"
     gene            complement(734..1870)
                     /locus_tag="P5201_08285"
     CDS             complement(734..1870)
                     /locus_tag="P5201_08285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014806452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HRV98572.1"
                     /translation="MIGFHRKKQERYRRFHERGKAAVLLKILQILPELHEGGVERHVL
                     WLSNGLAAGGHEVVVVSAGGKMERELRGVEHVRLPVHRKNPVTAAWAALRIARIAKAR
                     GVQLLHAHSRVPAWIAWWASCLSGIPWVATCHAFYSRNAGILPYRRARLLLCVSEAVR
                     SYFAEIFPEAECRVVYNGLPPTERVWRPDEGEAPRLLFVGRLVPKKGVDTLLDALSRS
                     RDVPWNLDIVGDGPSMEPLRQMAAQLGIADRVFFRGFQEEPERWMAECSCFLFPSHQE
                     GMGLVLMRAVQMGIPVLASDIPAVRELASSPEGLLQAGNAEGWRIALEDFLRTGTVGA
                     RFIAEKIPSIDAMVRSIEDVYAGIVRNGMEKATEAHRARFRRKK"
     gene            2007..>2459
                     /locus_tag="P5201_08290"
     CDS             2007..>2459
                     /locus_tag="P5201_08290"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRV98573.1"
                     /translation="MLFNSYVFLFLFLPATWFSFRLACSRRATNAALAVLLVASLLFY
                     SYWNPPFILLILFSILFNYAWGRGIERAVLQGGNVKGWLFSGLSVNLALIAYFKYANF
                     FMANLALLCGREWLHRDIFLPLGISFFTFQQMAYLVDCSKGLVGGHSFT"
BASE COUNT          478 a          793 c          656 g          532 t
ORIGIN      
        1 catcagccac atcgcctcga cgtagtcgcg cgcgtggccc cagtcgcgct tcgcgtcaag
       61 attgcccagg tgcagcttct cctgcaggcc aagggcgatg cgcgccgctg cccgcgtgat
      121 cttgcgggtg acgaaggtct ccccgcgcag gggcgattcg tgattgaaga ggatgccgtt
      181 gcaggcgtac atgccgtagg cctcgcggta gttcacggtg atccagtacc cgtacagctt
      241 cgccgcggcg taggggctgc gcggatagaa cggcgtcttc tcgttctgcg ggatctcctg
      301 caccttgccg tagagttcgc tggtggaggc ctggtagaag cgcgtacgct tctccaaacc
      361 gagaatccgt atagcttcaa gaatacgcag caccccgagt ccgtcgccgt tggcagtgta
      421 ctccggcgtc tcgaacgaga ccttcacatg gctctgcgcc gcgaggttgt aaatctcgtc
      481 gggctgcacc tcctggatga tccgtataat gttcgacgaa tcggtgagat ccccgtagtg
      541 aaggataagg cgccgttccg tctggtgcgg atcttcgtac aggtggtcga tcctccctgt
      601 gttgaaaaga gacgaccggc gcttcattcc gtgcacctcg taccccttgt tcagcagaaa
      661 ctcggccagg taggctccgt cctgtcctgt gatgccggtc aacaatgctt ttttcttcat
      721 ggacgcacct cgtttatttt ttccgacgga aacgcgctct gtgcgcttcc gtcgctttct
      781 ccatgccgtt tcgaactatg cccgcgtaca catcctcgat cgatcgcacc atcgcgtcga
      841 tcgacggaat cttttccgca ataaaacgtg ctcctaccgt acctgttcgc agaaagtcct
      901 caagagcaat acgccaacct tccgcgttcc cggcctgcaa caacccttcc ggtgacgacg
      961 cgagctcccg gaccgccgga atatccgagg ccagcacggg aattcccatt tgaaccgccc
     1021 tcatgagcac cagacccatc ccctcctgat gcgaagggaa gagaaaacag gagcattcgg
     1081 ccatccagcg ctccggctcc tcttgaaaac cgcggaagaa gactctgtcc gctatcccga
     1141 gctgcgcggc catctgacgt aacggctcca tcgaagggcc gtcgccgacg atgtcgagat
     1201 tccacggaac gtctcgcgaa cgagaaagcg cgtcgaggag agtatcgacg ccctttttcg
     1261 gcacgagcct gcccacgaaa agaagccgag gcgcttctcc ttcatccggt cgccacaccc
     1321 gctccgtcgg cggcagaccg ttatacacca cccggcactc cgcctcgggg aagatctccg
     1381 caaaatacga acgcaccgcc tcgctgacgc agagcagcag acgcgctctc cggtacggaa
     1441 ggattcccgc gttcctgctg taaaaggcat ggcacgtagc aacccacggt attccggaaa
     1501 gacaggacgc ccaccacgcg atccacgccg ggacgcggga gtgggcatgc agaagctgta
     1561 ctccgcgcgc ctttgcgatc cgcgctatac ggagagccgc ccacgccgcc gttaccgggt
     1621 ttttcctgtg caccggaagg cggacatgct ctacgccccg cagctccctc tccatctttc
     1681 caccggccga gacaacgaca acctcatgcc ccccggctgc aaggccgttg gagagccaca
     1741 gcacgtgccg ctcgacgccg ccttcatgca gttcgggaag aatctggaga atcttcaaga
     1801 ggaccgccgc ctttccacgc tcatggaagc gccggtagcg ctcctgtttt ttccgatgga
     1861 agcctatcat atttccctct ccttgtccaa aagaccggag cgccgaatta caaatcggaa
     1921 agtatcggat ataatacgca acatccgatg cggctctcca gaacagaatc ggaagaacgg
     1981 gccgcatgtc gatatggaga agaagcatgc tctttaattc ctatgttttc ctgtttctct
     2041 tcctgcccgc gacctggttt agtttccgcc tcgcatgctc caggagagcg acgaacgccg
     2101 ccctggccgt gcttcttgtt gcgtcgctgc ttttttattc gtactggaac cctccgttca
     2161 tcctcctcat tctcttttcg atcctcttca attacgcctg gggacgcggc attgaacggg
     2221 ccgtattaca aggcgggaat gtaaagggat ggctcttcag cggtttgagc gtcaacctgg
     2281 cgctgatcgc ctatttcaag tacgcgaact tcttcatggc caacctggcg ctcctctgcg
     2341 gccgcgagtg gctccaccgc gatatctttc ttccccttgg catctccttt ttcacctttc
     2401 agcagatggc ctaccttgtc gactgctcga aggggctggt tggaggacac tccttcacg
//
LOCUS       DAOTIL010000377         2454 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminobacteriaceae bacterium isolate AMR_MDS_3026
            NODE_50788_length_2454_cov_3.920383, whole genome shotgun sequence.
ACCESSION   DAOTIL010000377 DAOTIL010000000
VERSION     DAOTIL010000377.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868050
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminobacteriaceae bacterium (wastewater metagenome)
  ORGANISM  Aminobacteriaceae bacterium
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae.
REFERENCE   1  (bases 1 to 2454)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2454)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:19:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,224
            CDSs (total)                      :: 3,198
            Genes (coding)                    :: 3,184
            CDSs (with protein)               :: 3,184
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2454
                     /organism="Aminobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_50788_length_2454_cov_3.920383"
                     /isolate="AMR_MDS_3026"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:3034609"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(214..783)
                     /locus_tag="P5201_08295"
     CDS             complement(214..783)
                     /locus_tag="P5201_08295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013047490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008251 - tRNA-specific adenosine
                     deaminase activity [Evidence IEA];
                     GO_process: GO:0002100 - tRNA wobble adenosine to inosine
                     editing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deaminase"
                     /protein_id="HRV98574.1"
                     /translation="MEKEKLFHRMLDVIEGDIVPLTARGVSIGSKVFGAAVLRRDDLS
                     LVVAGTNHEAFSPLWHGEVYTIKLFYELQGRPDPSECLFLSTHEPCSMCISALSWAGF
                     KDIYYFFGYEQTQDDFNIPHDLRMLREVFGCATPTKENIYYTSRSLMDIAEGLEDLVT
                     ARARIARIKETYAGLSAVYQAGEKKMVLK"
     gene            complement(944..1273)
                     /locus_tag="P5201_08300"
     CDS             complement(944..1273)
                     /locus_tag="P5201_08300"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRV98575.1"
                     /translation="MRKIVLIAVLWGAMFGSAFAEPAPAAWKGDLRHVVERGGVSYSI
                     YADRTRLVEDCVPGAEQVLETFVHLVIESQNLVEIQQWNIRQDGSGYRVQDMYDYTLD
                     TFGMVDQ"
     gene            1398..1826
                     /locus_tag="P5201_08305"
     CDS             1398..1826
                     /locus_tag="P5201_08305"
                     /inference="COORDINATES: protein motif:HMM:NF012267.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HRV98576.1"
                     /translation="MTVKYLRVVNRDQCIGCFSCMYACSRMVHNAAGSGKAALRVKAY
                     TGVEGAFSIRVCARCEEPDCAAACPTGALTKAPGGGVRLKKELCISCMACVKACAIGA
                     LQWDEEEKRPLPCIHCGQCVKYCPNNVLALCEKEEECEPR"
     gene            1823..>2454
                     /locus_tag="P5201_08310"
     CDS             1823..>2454
                     /locus_tag="P5201_08310"
                     /inference="COORDINATES: protein motif:HMM:NF014750.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016625 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, iron-sulfur
                     protein as acceptor [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde ferredoxin oxidoreductase N-terminal
                     domain-containing protein"
                     /protein_id="HRV98577.1"
                     /translation="MNGAILHVDMTTGTHRMIDDPELFGEFLGGTAVATELLFRHGDP
                     KGDPLAPESPVIFAIGPFSSLFPVATKTVAMFKSPLSGELGESHAGGRMAMSLHEAGI
                     KALVITGKADTLSYLVIENDSAELKSASTFRGQSAAATERILREAEGKSGRKVSILRI
                     GPAGERLSPMACATVDGSRHFGRLGLGAVLGSKNLKAIVISGTRTLPLARP"
BASE COUNT          514 a          805 c          692 g          443 t
ORIGIN      
        1 cgagaatcac gaactcagcc ggagcggacg cccgcgcccc agacttaccc gactgcgcaa
       61 gatgacggag tggttcgacc gctttttgaa agcgaaagaa tagcggagga agttccgcga
      121 ctcgaacgca aatcccccct gcatcctcgc ggcgcagggg ggatttttcg ttcatcggat
      181 tgtaccgccg acgcgcatct tgcacatccc gcgctactta agcaccatct tcttctcgcc
      241 cgcctggtag acggcggaca gcccggcata cgtctccttt atcctggcga ttctcgccct
      301 ggccgtcacc agatcctcca gcccctccgc gatatccatg agcgaacggc tggtatagta
      361 gatattctcc ttcgtcggcg ttgcgcagcc gaagacctcg cgcagcatcc gcaggtcgtg
      421 cgggatgttg aagtcgtcct gcgtctgctc gtagccgaag aagtagtaga tgtctttgaa
      481 tcctgcccac gacagcgccg aaatgcacat cgagcacggc tcgtgcgtcg agaggaagag
      541 gcactccgaa gggtccgggc gcccctgcag ctcgtagaag agcttgatgg tgtacacctc
      601 gccgtgccac agcggggaga aggcttcgtg attcgttccc gcaaccacca gcgagagatc
      661 gtccctccgc agcaccgccg cgccgaacac cttgctcccg atgctcaccc cccgcgccgt
      721 cagcggaacg atatccccct cgatgacgtc gagcatacga tgaaacagct tctctttctc
      781 cacaaaaaca gctcccttca aagagtatcg gaacgcatga acggattccg gcgcagagtc
      841 aatcgcctcg ctccgctaca atcatctgtg cagcccggat aacatcccgc tgatacgagt
      901 cctcctgcac gggcctccac gaggcccact caggcaggac gattcactga tccaccatgc
      961 cgaacgtatc cagcgtatag tcgtacatat cctgaacgcg gtacccgctt ccatcctgcc
     1021 gtatgttcca ctgctggatc tcgaccaggt tctgcgattc gatcaccaga tgcacaaacg
     1081 tttcgagcac ctgctccgcg cctggaacac agtcctccac cagacgggtt ctgtccgcat
     1141 atatggaata ggagaccccg ccgcgctcca caacatgccg cagatctccc ttccacgccg
     1201 cgggagccgg ctccgcaaac gcgcttccaa acatcgcgcc ccaaaggacg gcgatcaaca
     1261 caattttcct catcgctccc acctccgttt attcgaaagg gctcgaattc ttttctcttt
     1321 gttcattgta gcagttgaca gggagaaaaa gacaggggat tattgcggag agagaaaaaa
     1381 agtgagaggt ggaacgcatg actgtcaaat atttacgagt tgtcaaccgt gaccagtgca
     1441 tcggctgctt cagctgcatg tacgcgtgca gccgcatggt gcataacgcc gcaggcagcg
     1501 gcaaggcggc gctgcgggtc aaagcctaca caggcgtgga gggcgcattc tcaatccgcg
     1561 tctgcgcacg gtgcgaggag ccagactgcg ccgcggcatg tccgacggga gcgctgacaa
     1621 aggccccggg cggcggagtg cgcctgaaaa aagagctctg catctcctgc atggcgtgcg
     1681 tcaaggcgtg cgcgatcggc gcgctccagt gggacgagga ggagaagcgc cccctcccgt
     1741 gcatccactg cggacagtgc gtcaagtatt gccccaacaa cgtcctcgcg ctctgcgaga
     1801 aagaagagga gtgtgaaccg cgatgaacgg cgccattctc cacgtcgaca tgacgacggg
     1861 aacgcaccgc atgatcgacg atcccgaact cttcggcgaa tttctgggag gaaccgccgt
     1921 cgccacggaa ctgctcttcc gccacggaga tccgaagggc gacccgctcg ctcccgagag
     1981 cccggttatc ttcgcgatcg gtccgttcag ctcgctcttt cctgtggcga cgaagaccgt
     2041 cgcgatgttc aaatcccccc tctccggcga actcggggag tcgcacgcag gcggccgcat
     2101 ggcgatgtcg ctgcacgaag cgggaatcaa ggcgctcgtg atcaccggca aagcggacac
     2161 actctcctac ctcgtcattg agaacgactc cgccgaactc aagtccgcct ccaccttcag
     2221 ggggcagtcc gccgccgcca ccgagcgtat tctgagagag gcggagggaa agagcggacg
     2281 aaaggtttcc atccttcgca tcggccccgc cggcgagcgc ctctcgccga tggcatgcgc
     2341 cacggtggac ggctcgcggc acttcggcag gctggggctt ggcgcggtcc tcgggagcaa
     2401 gaacctcaag gcgatcgtca tcagcgggac cagaacgctt ccgctcgccc gccc
//
LOCUS       DAOTIL010000378         2453 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminobacteriaceae bacterium isolate AMR_MDS_3026
            NODE_50819_length_2453_cov_3.406172, whole genome shotgun sequence.
ACCESSION   DAOTIL010000378 DAOTIL010000000
VERSION     DAOTIL010000378.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868050
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminobacteriaceae bacterium (wastewater metagenome)
  ORGANISM  Aminobacteriaceae bacterium
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae.
REFERENCE   1  (bases 1 to 2453)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2453)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:19:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,224
            CDSs (total)                      :: 3,198
            Genes (coding)                    :: 3,184
            CDSs (with protein)               :: 3,184
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2453
                     /organism="Aminobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_50819_length_2453_cov_3.406172"
                     /isolate="AMR_MDS_3026"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:3034609"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..381
                     /locus_tag="P5201_08315"
     CDS             <1..381
                     /locus_tag="P5201_08315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013047993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MMPL family transporter"
                     /protein_id="HRV98578.1"
                     /translation="FAGLISLKATLTLPGIAGIVLTIGMAVDGNVLIYERMKEELRAG
                     KTPLAAIDAGFKKALVTIFDANITTLIAAAVLFYFGSGPVRGFAVTLSIGIVSSVFCN
                     VLVTRSLLQYFMGGRATSSLAHRS"
     gene            401..1270
                     /gene="secF"
                     /locus_tag="P5201_08320"
     CDS             401..1270
                     /gene="secF"
                     /locus_tag="P5201_08320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009165500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031522 - cell envelope Sec protein
                     transport complex [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein translocase subunit SecF"
                     /protein_id="HRV98579.1"
                     /translation="MKIMFIDFMKHRRAALLVSLVCVVVSIGLVFTKGLNLGIDFTGG
                     NVVQVEFAQGVSVGDVRDVLASVGQGNAVIQSYSDIGFIIRISANEDEARKMVVDALR
                     AKYPDMKTVRLEKVGPVVGAELRKEAFVALFIALAGILAYITVRFQFRFAVVSVLALM
                     HDAIITLGVFSLTGMEISSSFIAAILTIVGYSLNDTIVVLDRVRENWKTLRGNGMLAL
                     LNNSINETISRTINTSMTTLLPVIALFLWGGPVLRSFSFALLIGITVGTYSSIFIASG
                     VLAEWWIKAPQKA"
     gene            1448..2122
                     /locus_tag="P5201_08325"
     CDS             1448..2122
                     /locus_tag="P5201_08325"
                     /inference="COORDINATES: protein motif:HMM:NF039842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5752 family protein"
                     /protein_id="HRV98580.1"
                     /translation="MKEHGTTLPFRFMECALLTLSTGVRAQSIRELRTALPQTPLSSI
                     YYHFWGRMLRPHIAESEFNNDFASWADSGLGDIELAEALSAINPVACADLGEIRSILE
                     DILDARLENGDFMSWKKSDREFYFIACTKMMFDTGREVSSLKDFPLAVKTASRQSFFH
                     HFIDGRRRSPEGKDDFSLWLEQFGEETEPLRKSLRAIDSYLFSLGELKGQVVSLLEKN
                     LHRRGK"
     gene            2125..>2453
                     /locus_tag="P5201_08330"
     CDS             2125..>2453
                     /locus_tag="P5201_08330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006584091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl transferase family 1"
                     /protein_id="HRV98581.1"
                     /translation="MSEMLKKYGEVVGFHAMDSLMVIADMLRGKKIVHVNSTKEGGGV
                     AEILSSMVPLSKELGLDVSWEVIEGNPDFFACTKTLHNSLQGMPGGLPEQQIEAYVET
                     NRTNAEKL"
BASE COUNT          520 a          689 c          723 g          521 t
ORIGIN      
        1 ttcgcgggac tgatctcgct gaaagcgacg ctgacccttc cgggcatcgc gggtatcgtg
       61 cttaccatag gcatggcggt ggacggcaac gtgctcatct atgaacgcat gaaggaggag
      121 ctgcgcgccg gcaagacgcc gctcgcggcc atcgacgccg gattcaaaaa ggccctcgtg
      181 acgatcttcg acgcgaatat cacgacgctc atcgcggcgg cggtcctctt ctacttcggc
      241 agcggccctg tcaggggttt tgcggtaacg ctcagcatcg gtattgtgtc gagcgtcttc
      301 tgcaacgtcc tcgtcacgcg ctctctcctc cagtacttca tgggcggacg ggcgacgagc
      361 tccctggcgc accgttccta agagaggtgg agaaaagact atgaaaatca tgttcattga
      421 ctttatgaaa catcgcaggg ctgcgctcct ggtcagcctt gtctgcgtcg tcgtcagcat
      481 cggtctggtc ttcacgaagg gactgaacct gggaatcgat ttcacgggag gcaacgtggt
      541 gcaggtcgag ttcgcgcagg gcgtctccgt gggcgatgtc cgcgacgtcc tggcttcggt
      601 gggccagggg aacgccgtta tccagtccta cagcgatatc ggcttcatca tccggatcag
      661 cgccaacgag gacgaagcca ggaaaatggt tgtcgacgcc cttcgggcaa agtatcccga
      721 tatgaaaacc gtccggcttg aaaaggtcgg acccgtggtc ggcgccgaac tgcggaaaga
      781 ggccttcgtg gcgctcttca tcgcgcttgc cggtatcctt gcgtatatca cggtccgctt
      841 ccagttccgt tttgccgtgg taagcgtcct cgcgctgatg cacgacgcga tcatcacgct
      901 tggcgtcttc agtcttaccg gcatggagat ctcctcgtcg tttatcgcgg cgatcctcac
      961 gatcgtcggc tactccctga acgacacgat cgtcgtcctc gacagggtcc gcgagaactg
     1021 gaaaacgctg cgcggaaacg ggatgctcgc cctgctgaac aattcgatca atgaaacgat
     1081 ctccagaacg atcaacacgt ccatgacgac cctgctgcct gtcatcgctc tcttcctctg
     1141 gggaggccct gtgctgcgca gcttctcgtt cgctctgctc atcggcataa cggtcggcac
     1201 gtacagctcc attttcatcg cctccggcgt gctcgccgaa tggtggataa aggcgcctca
     1261 gaaggcgtag ccgctgttct gcttttttgg gtattgtcat tccgaagcgc tcgccggaac
     1321 agaggcgagc gcttttttgg gcatgagaat ttgtagaaca aggcgtttat gtgttatact
     1381 cttacaataa tgtcatggcc acaacataaa aggagatgag atcggccggg aaaaggagga
     1441 aacagaaatg aaagaacacg gaacaacact tccgttcaga ttcatggaat gcgctctgct
     1501 cacgctgagt accggagtgc gcgcgcaatc catacgagag cttcgaaccg cgcttccgca
     1561 aacgccgctc agttccatct actaccactt ctgggggagg atgctgcgcc cccatatcgc
     1621 cgaatccgag ttcaataacg acttcgcctc gtgggccgac agcggccttg gagatataga
     1681 gctcgccgag gcgctcagcg cgatcaaccc cgtagcgtgc gccgatcttg gcgagatacg
     1741 ctcgatcctt gaggacattc tggacgcgcg tcttgagaac ggcgatttta tgagctggaa
     1801 gaagagcgac agggaattct actttatcgc ctgcaccaag atgatgttcg acacggggcg
     1861 cgaggtctcc tcgctgaagg acttcccgct tgcggtgaag acggcgagcc gtcagagctt
     1921 ctttcatcac ttcatcgacg gcaggagacg ctcccccgag gggaaggacg acttttctct
     1981 ctggctcgag cagttcggcg aagagacgga gccgcttcgg aagtcgctca gggcgatcga
     2041 ttcgtatctc ttctcactcg gtgagttgaa ggggcaggtt gtttcccttc ttgaaaagaa
     2101 tctgcacagg agggggaaat aacgatgtcc gagatgctga aaaaatacgg cgaggttgtc
     2161 ggcttccatg cgatggacag cctgatggtc atcgcggata tgctgcgggg gaagaaaatc
     2221 gtccacgtga actcgacgaa agagggggga ggcgtggcgg agattctctc ctcgatggtc
     2281 ccgctctcga aggagctcgg actcgacgtc tcctgggagg tcatcgaagg gaaccccgac
     2341 tttttcgcct gcacaaagac cctgcacaac tcactgcagg ggatgcccgg aggtctgccg
     2401 gaacaacaga tcgaagcgta cgtcgaaacg aaccgcacga acgcggagaa gct
//
LOCUS       DAOTIL010000379         2449 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminobacteriaceae bacterium isolate AMR_MDS_3026
            NODE_50953_length_2449_cov_2.605263, whole genome shotgun sequence.
ACCESSION   DAOTIL010000379 DAOTIL010000000
VERSION     DAOTIL010000379.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868050
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminobacteriaceae bacterium (wastewater metagenome)
  ORGANISM  Aminobacteriaceae bacterium
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae.
REFERENCE   1  (bases 1 to 2449)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2449)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:19:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,224
            CDSs (total)                      :: 3,198
            Genes (coding)                    :: 3,184
            CDSs (with protein)               :: 3,184
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2449
                     /organism="Aminobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_50953_length_2449_cov_2.605263"
                     /isolate="AMR_MDS_3026"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:3034609"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..277)
                     /locus_tag="P5201_08335"
     CDS             complement(<1..277)
                     /locus_tag="P5201_08335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005660366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HRV98582.1"
                     /translation="MEAVSRCNTRRLVWTALLSALAAVLMYIDMALPIFPRFLKIDIS
                     DLPALVASFSYGPVAGVFTELIKNLIHMFSTSSAGIGEAANFLMGSAL"
     regulatory      complement(346..501)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00050"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="FMN riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="flavin mononucleotide"
                     /db_xref="RFAM:RF00050"
     gene            648..1616
                     /locus_tag="P5201_08340"
     CDS             648..1616
                     /locus_tag="P5201_08340"
                     /EC_number="1.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013047425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004073 - aspartate-semialdehyde
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0008652 - amino acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate-semialdehyde dehydrogenase"
                     /protein_id="HRV98583.1"
                     /translation="MKIAVVGATGEVGRMMTRVLQEQNVKPGKIDFYASAKSAGSSVE
                     FYGKKYEVKELAKKAMKEGYDYLLFSAGGSVSREFAPIAAEAGSVVIDNSSAFRMDSA
                     IPLVVPEINGDLLKGYTQGIVANPNCSTIQMVLGLYRIHERFGIRSIVVSTYQSVSGA
                     GKNGMNELEEQMKGTIAPKKFIKQIHLNVVPQIGALLENGFTDEEMKMVNETRKILRD
                     PDISIWATTIRVPVLYCHSESIYVETRTAFDMDGLAEALEASENVIHTDEMFSPIDVA
                     GSDTVYVGRLRAFDATRFLMWNVADNIRVGAATNAVRILLKHRASN"
     gene            1621..>2449
                     /locus_tag="P5201_08345"
     CDS             1621..>2449
                     /locus_tag="P5201_08345"
                     /inference="COORDINATES: protein motif:HMM:NF015509.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AEC family transporter"
                     /protein_id="HRV98584.1"
                     /translation="MHGFFVILPVVLVMAAGWMLGKRRVVLPEAFAQINKAIYWVAIP
                     ALILRMTSSADMSTLADKNMVLAVYASFLLAPPFAWFAARMAGEKREKKATSTLMLIR
                     SNTVFMGLPVVTIAVGMKGVEVLSLYLAFTFIGYQIISISWAQLALSGGISRSTVKET
                     LKNLAKNPLVVSSLAGFGLSVFGWNAFPSWLDETLKLLGNTASGMALLSLGASLKLDG
                     LSGILPRVWRDATLKLLFLPALTWCFFLLLPVDPVVFRTVLLISAMPVAVDCFILSQV
                     "
BASE COUNT          501 a          701 c          716 g          531 t
ORIGIN      
        1 cgagggccga gcccatcagg aaattcgccg cctcgccgat tcccgcggag gatgttgaga
       61 acatgtggat gaggttcttg atcagctctg tgaagactcc ggcaaccggg ccgtaggaga
      121 acgaggcgac cagcgccgga agatcggaga tgtcgatctt cagaaaacgg ggaaatatcg
      181 gcagcgccat gtcgatgtac atcagcaccg cggcaagtgc ggagagcagc gcggtccaga
      241 cgagacggcg cgtgttgcaa cgggatacgg cttccatagt atgatcaagt cctttcaaaa
      301 aaagtagccc cggacgcgac gcatccgggg cacgtgtgca gtcttcaaca ccctcttcca
      361 tcctgactac ggtctgtccc gaaggacatc cttacagtcg gctccggatt cgcaccggat
      421 cggcctttcg gctcgcgggc ttggagccgt ttccggcccg tcaccgccgg tggggagttc
      481 caccccgccc cgaagatttt ccactctatt gtacaccgct ttttcgaaaa tttctattga
      541 catgggcgcg caatcttcct ataatcccgg aaaaccgcga aagtgcaggc tcgttcctcc
      601 acggggcggg ctttttttga ttgcattcac gaaaggagat gttcctattg aaaatagcag
      661 tagtcggcgc taccggagaa gttggacgca tgatgaccag ggtgctccaa gagcagaacg
      721 tgaagcccgg gaagatcgat ttttacgcct cggcgaagag cgccggttcc agtgtggagt
      781 tttacgggaa aaaatacgaa gtcaaagagc tcgcgaagaa ggcgatgaag gaagggtacg
      841 actacctgct cttctcggcg ggaggctccg tctcccgtga atttgctccc atcgccgccg
      901 aggcgggaag cgtggtcatc gacaactcct ctgcgttccg gatggattcc gcaattcccc
      961 tcgtcgtccc cgagatcaac ggggatctgc tcaaaggcta tacgcagggg atcgttgcaa
     1021 acccgaactg ctcgaccatc cagatggtgc tggggctcta caggattcac gagcgtttcg
     1081 gcatccgctc catcgtggtg agcacctacc agtccgtctc cggagcaggg aagaacggta
     1141 tgaacgagct tgaagaacag atgaaaggga ctatagcgcc gaagaagttc atcaagcaga
     1201 ttcacctcaa cgtcgtgccg caaataggcg cgttgctgga gaacggtttc accgacgaag
     1261 agatgaagat ggtcaacgag acgaggaaga tcctgcgaga ccctgacatt tcaatctggg
     1321 caacaacgat ccgggtgcct gtgctctatt gccactccga gtcgatctat gtggagacga
     1381 ggacagcgtt cgatatggac ggccttgcgg aagcgctcga agcgtcggaa aacgtcatcc
     1441 atacggatga gatgttttct cctatagacg tcgccggaag cgataccgtc tatgtcgggc
     1501 gccttcgggc gttcgacgcc acccgcttcc tgatgtggaa cgtcgcggac aacatccgcg
     1561 tcggagccgc aacgaacgcc gttcgcatcc tgttgaaaca ccgcgcttcg aactagcggt
     1621 atgcacggtt tttttgtcat ccttccggtg gtgcttgtca tggccgccgg gtggatgctc
     1681 ggcaaacggc gtgtcgttct ccccgaagcc tttgcgcaga tcaacaaggc tatctactgg
     1741 gtcgccatac ccgcgctcat tctgcgaatg accagcagcg cggacatgtc gacgcttgcc
     1801 gacaagaaca tggtgcttgc ggtctatgca tcctttcttt tggcgcctcc gtttgcatgg
     1861 ttcgcggccc ggatggcagg ggaaaaacga gagaagaaag cgacgtccac gctgatgctc
     1921 attcgttcga ataccgtttt tatggggctg cccgtcgtca cgatcgcagt gggtatgaag
     1981 ggagtagagg tgctctcgct ctacctcgcg tttacgttca tcgggtacca gatcatctct
     2041 atctcctggg cccagcttgc gctctccggc ggtatttcgc gcagcacggt gaaagagacg
     2101 ctgaagaacc tggcgaagaa cccgcttgtc gtctccagcc tcgccgggtt cgggctctcg
     2161 gttttcgggt ggaacgcctt cccgtcctgg ctggatgaga cgttgaagct gctcggcaac
     2221 acagcaagcg gcatggcgct gctctcgctc ggcgcgtcgc tcaagctcga cgggctttcg
     2281 ggaatactgc cccgcgtctg gcgcgacgca acgctgaagc tcctctttct cccggcgctc
     2341 acctggtgtt tcttccttct gctgccggtc gatccggtcg tcttccggac ggtccttctc
     2401 atttcggcca tgcctgtcgc ggtggactgc ttcatcctct cgcaggttc
//
LOCUS       DAOTIL010000380         2449 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminobacteriaceae bacterium isolate AMR_MDS_3026
            NODE_50958_length_2449_cov_2.165831, whole genome shotgun sequence.
ACCESSION   DAOTIL010000380 DAOTIL010000000
VERSION     DAOTIL010000380.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868050
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminobacteriaceae bacterium (wastewater metagenome)
  ORGANISM  Aminobacteriaceae bacterium
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae.
REFERENCE   1  (bases 1 to 2449)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2449)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:19:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,224
            CDSs (total)                      :: 3,198
            Genes (coding)                    :: 3,184
            CDSs (with protein)               :: 3,184
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2449
                     /organism="Aminobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_50958_length_2449_cov_2.165831"
                     /isolate="AMR_MDS_3026"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:3034609"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..181
                     /locus_tag="P5201_08350"
     CDS             <1..181
                     /locus_tag="P5201_08350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006583169.1"
                     /note="Members of this family of hydrolases with an active
                     site Cys residue belong to MEROPS family C26; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="gamma-glutamyl-gamma-aminobutyrate hydrolase
                     family protein"
                     /protein_id="HRV98585.1"
                     /translation="GMVIAGVYEEKNLVEIVELQDHPWFVGVQFHPEFRSRPVKPHPV
                     FMGFIGAAVQRSQSD"
     gene            196..1215
                     /locus_tag="P5201_08355"
     CDS             196..1215
                     /locus_tag="P5201_08355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013048853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRV98586.1"
                     /translation="MRRTFLVLVCVVLLLMGAVLPLFAQEDSSTFHVMSRVEQLVYGG
                     ERTGGLVDRLNSVERDMFGRELPGSIAERQTALLTFIEKGTEYQPSLLFKLGVAEWAV
                     ERQIDSLSPVVKRVDALEVQLEGTPQVERPVTMRLERVLGLLLSEPVTAAEVELPAAT
                     IIRASLAHSLSPKTAKKGDPVDMVLHSDLVVGANLIAPKGSRVSAVVTEVTKPRSFGR
                     PGEVKVGVESLFSIGSQPIPLTFGEESRQAVEAESAQLAAAGTSVVGALLLGPVGLVG
                     GFLVRGDLKELPAGTVVFSQTADAVKVGAYPVPPGLQGLLKNPEKAEPAQPGTSESAT
                     NETTP"
     gene            1233..1670
                     /locus_tag="P5201_08360"
     CDS             1233..1670
                     /locus_tag="P5201_08360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013048852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRV98587.1"
                     /translation="MFEGKRKKIVFWVLLLLLFTGGVSRAFDLGDLVGVIGGGFVVST
                     IAGPINDFINTITLNKGVKVEGHTKVVPIVSIGSGTHIGAAQIAGPRGEPVGMTKAVA
                     QIEATFRDRLRVKILVPVDSENPVQRFRRVQGVGVSAIIDFRL"
     gene            1704..2081
                     /locus_tag="P5201_08365"
     CDS             1704..2081
                     /locus_tag="P5201_08365"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRV98588.1"
                     /translation="MKRLFFVIMFLCCLSAPSFGSGSIVEATSILERWTAFHWGRDCL
                     VWLVHYPEELIDPWVAAEAARSGMSEAESAQYRASFTSELRIAETEPFLLLCTSSALA
                     RLTWARLRPLSSCRRTRGRKFLR"
     gene            2168..>2449
                     /locus_tag="P5201_08370"
     CDS             2168..>2449
                     /locus_tag="P5201_08370"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRV98589.1"
                     /translation="MIIRNLGIQEEQHFVFDRADGPLVAAAEPEVVVVELPPAPKKEP
                     VKPQPPKREKPTPTPPPPPPVATPQELAELKPEPVVVDLPAPEKEKPVLY"
BASE COUNT          482 a          632 c          770 g          565 t
ORIGIN      
        1 ggggatggtg atcgcgggcg tctacgagga gaaaaacctc gtggagatcg tcgagttgca
       61 ggatcatcca tggtttgtcg gcgtccagtt ccacccggag ttccgctccc gccccgtaaa
      121 gccgcatcct gtgttcatgg gctttatcgg tgcagcggtg cagcggagtc agtcagattg
      181 aaggagggga aacgaatgag acgtactttt ctcgttcttg tatgcgttgt actgcttttg
      241 atgggcgcgg tattaccgtt gttcgcccag gaagattctt cgacttttca tgttatgagc
      301 cgtgtggagc agcttgtgta cggcggcgaa cgcaccggcg gactggtcga ccgcctgaat
      361 tccgtggaaa gagatatgtt cggaagggag ttgcccggaa gtatcgccga acggcagaca
      421 gccctgctta cgtttatcga gaagggaact gagtaccagc cgtcgcttct tttcaagctt
      481 ggcgtggcgg aatgggcggt cgagcggcaa attgactccc tctctcctgt tgtaaaaaga
      541 gtggatgctc ttgaagtcca gctcgaagga acgccgcagg tggaacggcc ggtcacgatg
      601 agactcgaac gggtcctcgg acttcttctc tccgaaccgg tgacagccgc ggaagttgaa
      661 ctccccgcag cgacgattat tcgggcgtct ctggcgcact cgctctcgcc gaagacggcc
      721 aaaaagggtg atccggtcga catggtgctc cattccgacc tggtcgtcgg ggcaaacctg
      781 atcgctccga agggcagccg agtctctgcg gtagtgacgg aggtgacaaa accccggagc
      841 ttcggacgtc ccggagaagt aaaggttgga gtagagtcgt tgttttcgat cggttcccaa
      901 ccaattccgc tgacattcgg cgaagagtcg aggcaggcgg tcgaagcgga atcggcgcag
      961 cttgccgccg caggcacgag tgtggtgggc gcattgctcc tgggtcctgt agggctggtc
     1021 ggcggttttc tcgttcgcgg cgacttgaag gagcttcccg ccggaaccgt tgtcttttcg
     1081 caaactgccg acgcggtaaa agtgggggca tacccggtgc cgccgggatt gcaggggctg
     1141 ttgaaaaacc cggagaaagc cgagcccgcc caaccgggaa cgagcgaatc ggctactaac
     1201 gaaacaactc cttgaaaagg aggtaattcg tcatgttcga ggggaaacga aagaaaattg
     1261 tgttttgggt tcttctgctg ctcctgttca cgggaggggt ttctcgtgcg ttcgatcttg
     1321 gcgatctcgt cggcgtcatc ggaggaggtt ttgtcgtctc gactatcgca gggcctatca
     1381 acgacttcat caatacgatt acgttgaaca agggagtcaa ggtggaaggg catacaaagg
     1441 ttgttcctat tgtttccatt ggttccggaa cgcacatagg cgcggcgcag atcgccgggc
     1501 cgagaggaga acctgttggg atgacaaagg cagtggctca gatagaggca accttccggg
     1561 atcgtctgcg cgtgaagata ctggtgccgg tggattctga gaatccggtg cagcgcttcc
     1621 ggcgggttca aggagtcgga gtttcggcga tcattgattt tagactatag ggtgatcaat
     1681 ctcttgtttt tggggtggcg ttcatgaaac gactgttttt tgttattatg tttctttgct
     1741 gcctttctgc cccgtctttc gggagcggtt ccatcgttga ggcgacgtcc attcttgaac
     1801 gatggacggc ctttcactgg ggtcgcgact gtcttgtatg gcttgtgcac tatccggagg
     1861 agcttattga cccctgggtt gctgccgagg ccgcgcgcag cggaatgagc gaggcggaga
     1921 gcgctcagta ccgcgcttcc ttcacctccg agctgaggat agcagagacg gaaccttttc
     1981 tgctactgtg tacgtcttcg gccctcgccc gcttgacctg ggctcgtttg cgtccgctgt
     2041 ccagctgcag acgaacgagg ggaagaaagt tcctccgctg agttatgaac gtaagttcga
     2101 tcagccgctc tccggtatcg tgcaaggcct tgtctttttc ccgaaacagc cggagaaggg
     2161 cttcagtgtg attatccgaa accttggcat ccaggaagag cagcattttg tttttgatcg
     2221 tgcggacggc cccttggttg ccgcggcgga accggaagta gtggtggtcg aactgccgcc
     2281 ggcgccgaag aaggaacccg tgaagcctca gccgccgaag cgcgagaaac cgactcccac
     2341 tccgcctcct ccccctccgg tggcgacgcc tcaagaactt gcggagctga aaccggaacc
     2401 tgtggttgtc gatctgcctg ctccggagaa ggagaagccc gttctctac
//
LOCUS       DAOTIL010000381         2441 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminobacteriaceae bacterium isolate AMR_MDS_3026
            NODE_51168_length_2441_cov_3.254401, whole genome shotgun sequence.
ACCESSION   DAOTIL010000381 DAOTIL010000000
VERSION     DAOTIL010000381.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868050
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminobacteriaceae bacterium (wastewater metagenome)
  ORGANISM  Aminobacteriaceae bacterium
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae.
REFERENCE   1  (bases 1 to 2441)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2441)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:19:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,224
            CDSs (total)                      :: 3,198
            Genes (coding)                    :: 3,184
            CDSs (with protein)               :: 3,184
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2441
                     /organism="Aminobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_51168_length_2441_cov_3.254401"
                     /isolate="AMR_MDS_3026"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:3034609"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1220
                     /locus_tag="P5201_08375"
     CDS             <1..1220
                     /locus_tag="P5201_08375"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: protein motif:HMM:NF012492.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecG"
                     /protein_id="HRV98590.1"
                     /translation="APMNRLLQGDVGSGKTVVALIALLAALDGECQGALLAPTEILAR
                     QHFLRLSPVFTALGIPCALLTGSLGVKEKKEVYAGLESGEIAVVIGTHALFSEGVSFA
                     RLGLAIVDEQHRFGVLQKHTFRAKGSAPHVLVMTATPIPRTLTLTVYGDLSVSVISGM
                     PPGRKGVKTSAVSPKKLPAVLRFLKERFHLGERAYWICPLIEESESDLSAATARFEEL
                     SAEFGAYGVGLLHGRLPLAEKERAMEDFRSGVTRLLVSTTVVEVGVDVPEATVMVVED
                     AVRFGLSQLHQLRGRIGRGNAEGFCILVCTPSTPESRQRIEAFCSSDDGFAIAEADLR
                     LRGPGEVCGVRQSGVTDFRIADLLKDQEILRLARAEAFALVGEDPELRNTPLLADRVF
                     HVLGRSLNLVETA"
     gene            1204..1662
                     /locus_tag="P5201_08380"
     CDS             1204..1662
                     /locus_tag="P5201_08380"
                     /inference="COORDINATES: protein motif:HMM:NF014668.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006282 - regulation of DNA repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="regulatory protein RecX"
                     /protein_id="HRV98591.1"
                     /translation="MSKQPERKKIPGAKNPEAYLHSLLSNGVWTKAVVLRKLQGRGIP
                     EEEAEKLIDKYEEAGFFDDGAYALLFVESHPEWGVYRLRDELRRRGVAQEHILKALEG
                     KDEDENAAALAGEWIRMGIDRRKIGERLLRRGFSGNACRNALERACEGEL"
     gene            1758..>2441
                     /locus_tag="P5201_08385"
     CDS             1758..>2441
                     /locus_tag="P5201_08385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013048785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rnase Y domain-containing protein"
                     /protein_id="HRV98592.1"
                     /translation="MNFFTAIVALVVGVAAGIGAGFLLSKSFGIKNIENAKAQARAIL
                     REAAMAAEQGKREKLTEAKEEIFKLRQEVEREGKERRSELQRAERRLEQKEENLDKKL
                     DRVAHREEELKAKQEELENRMLELDEIKKKQLERLEEIARMTSEEARELLLRQTEEDA
                     QHFLGLRIKELEEKTKREADRKAREIIVTAVQRCAVEHTSESAVSVVHLPSDEMKGRI
                     IGREGRNIRT"
BASE COUNT          590 a          577 c          763 g          511 t
ORIGIN      
        1 cggcgcctat gaatcgcctt cttcagggag atgtaggatc gggtaagact gttgttgcac
       61 ttatcgcatt gctcgctgcg ctcgacggag agtgccaggg agctcttctg gccccgaccg
      121 agattcttgc ccgccagcac ttcctccgcc tttcacctgt ttttactgct cttggaattc
      181 cgtgcgcctt gctgaccggc tctctcggcg tcaaagaaaa aaaagaggta tacgccggtc
      241 ttgaatccgg cgaaatagcc gttgttatag ggacgcacgc tctcttctcg gaaggagtat
      301 cgtttgctcg attgggcctt gccatcgtcg acgaacagca ccgtttcggc gtcctccaga
      361 agcatacctt ccgcgccaag ggaagcgcgc cgcacgtttt agtgatgacg gcgacgccga
      421 ttcctcgcac gctgacgctg accgtctacg gagatctctc tgtatccgtg atctcaggta
      481 tgccgcccgg aagaaaagga gtgaagacga gtgcggtttc gccgaaaaaa ctgcccgcag
      541 ttctgcgttt tttaaaagaa cgatttcact tgggagagcg cgcgtactgg atatgccctc
      601 tcatcgaaga gagcgaatcc gatctttccg cggcgacggc gaggttcgaa gagctctccg
      661 cggaattcgg agcctacgga gtggggctgc tccacggaag actcccgctt gcggagaagg
      721 aacgggcgat ggaggatttt cggagcggcg tcacacggct tcttgtttct accacggtgg
      781 tcgaggttgg ggtggatgtt cccgaggcga cagtgatggt tgtcgaggac gcggtccgat
      841 tcgggctttc ccagcttcat cagctgagag gtagaatcgg cagaggaaat gcggagggat
      901 tctgcattct tgtgtgtacg ccgtcgactc cggaaagcag gcagagaatc gaggcgttct
      961 gctcctcgga tgacggcttt gctattgcgg aggcggatct ccggcttcgt ggtcccgggg
     1021 aagtctgcgg cgtccgccag tccggtgtga cggattttcg tattgccgat cttttaaaag
     1081 accaggagat cctccggctt gcgcgagcgg aagcattcgc tcttgtcggg gaggatccgg
     1141 aactcaggaa tacgccgctt ctggcggatc gcgtatttca tgtcctcggg aggtcgctga
     1201 atcttgtcga aacagcctga aagaaaaaaa atacccggcg caaagaaccc ggaggcatat
     1261 ctgcattcgc tgctttcaaa cggcgtctgg acgaaggctg tcgttcttcg caagcttcaa
     1321 ggcaggggga ttccggagga agaagcggaa aagctcatag acaagtacga ggaagccggt
     1381 tttttcgatg acggcgccta cgcccttctt tttgtggaat cgcatccgga atggggagta
     1441 tacagacttc gggacgagtt gcggaggaga ggcgttgcgc aggagcatat actcaaggcc
     1501 cttgaaggaa aagatgagga tgagaatgcg gctgcgcttg ccggagaatg gatccggatg
     1561 ggaatagatc ggcgaaagat aggcgagaga ctgcttcggc gcggtttttc ggggaatgca
     1621 tgcagaaatg ctcttgaaag ggcttgtgag ggggaacttt gacctgtaaa ataaagaggt
     1681 catggaagag agtgccttcc atgacgtgaa aaaacaaagg caccttgaaa atataatcga
     1741 ggagtgatca tgatactatg aactttttta ctgcaattgt ggcgctcgtt gtaggagtcg
     1801 ccgctggaat tggagcgggg ttcctgcttt ccaaatcttt tggaatcaag aacatcgaaa
     1861 atgcaaaggc gcaggctcgg gctattttgc gggaagccgc catggcggcc gagcagggga
     1921 aacgcgagaa actcaccgag gcgaaagaag agatcttcaa acttcgccag gaggtcgaac
     1981 gcgaaggtaa ggaacgccgt tcggaacttc aacgcgccga acgtcgtttg gagcagaaag
     2041 aggagaacct ggataaaaag ctggaccgcg ttgcccaccg tgaagaggag ttgaaggcaa
     2101 agcaggaaga gctggagaac aggatgctcg aactcgacga aatcaaaaag aaacagctgg
     2161 aacgcctcga agaaatcgcc cgcatgacga gcgaggaagc aagggaatta cttcttcgcc
     2221 aaacggagga ggatgcgcag catttcctcg gtcttcgtat caaggaactt gaagagaaaa
     2281 caaagcgcga ggcggacaga aaagcgcgtg agattatcgt gacggcggta cagcgctgtg
     2341 cagtcgagca cacatcggaa tcggcagtga gcgtggtgca tcttccctct gatgagatga
     2401 agggaaggat tatcggcagg gagggacgga acatccgtac c
//
LOCUS       DAOTIL010000382         2436 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminobacteriaceae bacterium isolate AMR_MDS_3026
            NODE_51311_length_2436_cov_3.713566, whole genome shotgun sequence.
ACCESSION   DAOTIL010000382 DAOTIL010000000
VERSION     DAOTIL010000382.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868050
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminobacteriaceae bacterium (wastewater metagenome)
  ORGANISM  Aminobacteriaceae bacterium
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae.
REFERENCE   1  (bases 1 to 2436)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2436)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:19:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,224
            CDSs (total)                      :: 3,198
            Genes (coding)                    :: 3,184
            CDSs (with protein)               :: 3,184
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2436
                     /organism="Aminobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_51311_length_2436_cov_3.713566"
                     /isolate="AMR_MDS_3026"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:3034609"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..801
                     /locus_tag="P5201_08390"
     CDS             <1..801
                     /locus_tag="P5201_08390"
                     /inference="COORDINATES: protein motif:HMM:NF014094.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATPase subunit"
                     /protein_id="HRV98593.1"
                     /translation="LRIIATGVGDREALRRRAASVPITLADPKKLLAAHTFRDVIEAI
                     KGYPLEHVLTDPLRQASETYAGGSVNLFRAKMAMDAFFMTRILSAGEKLPGSEGKGIR
                     SIFGTRADLINLYWIYRGRRFFAMTPEEALAITLPVRYTLNFETLRSFAFAPDIPAML
                     RLIQECPYGAAFTTALGESTLEVSEIILEHNLYRILWRAATKIFASGTSGVHVVLAYL
                     ILRELEVKDLFTIIEDIRYDYDKKRAREFLIHPGANAFTAGDRGMSWQ"
     gene            792..>2436
                     /locus_tag="P5201_08395"
     CDS             792..>2436
                     /locus_tag="P5201_08395"
                     /inference="COORDINATES: protein motif:HMM:NF013647.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0033179 - proton-transporting V-type
                     ATPase, V0 domain [Evidence IEA];
                     GO_function: GO:0015078 - proton transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATPase 116kDa subunit family protein"
                     /protein_id="HRV98594.1"
                     /translation="MAVVEMTGISMVGPREEIERVAAGLLSLGNFEPVPLDFAMEKHP
                     GALSGSGGSRIATFRENPYDELLEVLCEIQALVSDEAAQGRTSGAIFPERGGGASARP
                     QISSGELYDRLLPSFASLPEACGLNRRHESGSWTMPPGGSGERSIVCPSLEEASARVK
                     GLQRRIKFWRERSEKLQEEREKLLAAKVFLDALASAGLDRTKSPPGAGGGKGRPDVAS
                     GPPAAAPGSTAWLERALDTRYLSLTFGRLSTDNYKRLAEMAGITPLLVFPVVTEKDYV
                     LVAIFCSRDYHPEAEKLFRVVYLKEYVLQEILEGSGGAMKEYLEEQLGRSERALRALR
                     EAPRQYLERHRKELELLYCAVYAKQRIYSLCRNRGELGDVFIVSGLVPSPTLPQVEDV
                     IERFGPNTLLLTEKDDALKRRGRRLPTLLKNTFPVRVFQEIVALYSLPSYDDIDPSPL
                     VALSFCIFFGFMFGDVGHGLLISAGAWFLARKGILKKAFSSVLQIAGFSAAAFGFLYG
                     SIFGSEELIPALWTSPMHGINDLILASLVTGTGFLSLGIA"
BASE COUNT          414 a          751 c          811 g          460 t
ORIGIN      
        1 ctgcgcatca tcgcgacggg cgtgggcgac agagaggcgc tgcgacgtcg tgcggcctcc
       61 gtgccgatta cgctggcgga cccgaaaaag ctgctcgccg cgcacacctt ccgcgacgtg
      121 atcgaggcga tcaaaggata tcctttggag cacgtgctga cggatcctct gcggcaggcg
      181 tccgagacgt acgcgggagg atcggtcaac ctgttccggg cgaagatggc gatggacgcc
      241 ttcttcatga cgcggatcct gtcggcggga gaaaaacttc ccggaagcga ggggaagggc
      301 atccggagca tcttcggcac ccgggccgac ctgatcaacc tctactggat ctaccgggga
      361 cgacgttttt ttgcgatgac gccggaggaa gcccttgcga tcacgctgcc ggtgcggtac
      421 acgctgaatt tcgaaacgct gcgttcgttc gccttcgccc cggacattcc ggcgatgctg
      481 cgtctgatac aggagtgtcc gtacggagcg gcgttcacga cggcgctcgg cgagtcgacg
      541 ctcgaggtga gcgagattat tctggagcac aacctctacc gcatcctgtg gagggcggcg
      601 acgaagattt tcgcctcggg aacttccggg gtgcatgtgg tgctggcgta tctgatactg
      661 cgggaactgg aggtgaagga tctgttcact attattgagg acatccggta cgactatgac
      721 aagaagaggg cgcgcgaatt cctgatccac cccggggcga acgccttcac agccggagat
      781 cgggggatgt catggcagta gtggagatga cgggaatttc gatggtcggt ccgcgggagg
      841 agatcgagcg cgttgcggcg ggtctgctct ccctcgggaa cttcgagccc gtcccgctgg
      901 atttcgctat ggagaagcac ccgggggccc tgtcgggctc cggcgggtcg cggatcgcga
      961 cgttcaggga gaacccgtac gacgagcttc tggaggtgct ctgcgagatt caggcgcttg
     1021 tgtcggacga agcggcgcag gggaggacct ccggcgcgat cttccccgag agggggggcg
     1081 gcgcgtccgc gcgtccgcag atttcctcgg gcgagcttta cgaccgtctg ctcccgtcct
     1141 tcgcctccct gccggaagcg tgcggactga accggcggca cgagagcggc tcgtggacga
     1201 tgccgcccgg cggctccggc gagcgcagta tcgtctgtcc ctctctcgaa gaggcgtcgg
     1261 cccgcgtcaa ggggctccag aggagaatca aattctggcg ggagcgttcg gaaaagctgc
     1321 aggaagagcg tgagaagctc ctggccgcga aggtctttct ggacgcgctc gcttccgcgg
     1381 ggctcgacag gacgaagagc cctccgggcg cagggggcgg taagggacgt cccgacgtcg
     1441 cctccgggcc tccggccgcc gcgcccggct cgacggcatg gctggagcgg gcgctcgaca
     1501 cgcgctatct ctccctgacc ttcggacgcc tgagcacgga caactacaaa cgtctggcgg
     1561 agatggcggg aatcacgccg ctgctggtct ttccggtggt tacggagaag gactacgtgc
     1621 tggtggccat cttctgctcg cgcgactacc acccggaggc ggagaagctg ttccgcgtgg
     1681 tctacctgaa ggagtacgtc ctgcaggaga tcctcgaagg ctccggaggc gcgatgaaag
     1741 agtatctcga ggagcagctc gggcgttcgg agcgtgcgct ccgcgcactc cgcgaggctc
     1801 cgcggcaata tctggaacgc cacagaaaag agctcgaact gctctactgc gccgtgtatg
     1861 cgaagcagcg gatctactcc ctctgccgga accgcggcga gctgggggac gtgttcatcg
     1921 tttcgggcct cgttccctcc ccgacgctgc cgcaggtgga ggacgtcatc gaacgcttcg
     1981 ggccgaacac ccttctgctg accgagaagg acgacgcgct gaagcgccgg gggcgcaggc
     2041 tccccacact tttgaagaac acctttcctg tacgggtctt tcaggagatc gtggctctct
     2101 acagcctccc ctcctacgac gacatcgacc cttcgcccct tgtggcgctc tcattctgca
     2161 tctttttcgg cttcatgttc ggcgacgtcg gacacggcct cctgatctcc gcgggcgcct
     2221 ggttcctcgc ccggaagggc attctgaaaa aggcgttctc gtcggttctg caaatcgccg
     2281 gtttttccgc ggcggcattc ggctttctct acgggagcat cttcggctcc gaggagctca
     2341 tacccgctct ctggacatcc cccatgcacg gcatcaacga cctgattctc gcctccctcg
     2401 tcacggggac gggcttcctg agcctcggga tcgcgc
//
LOCUS       DAOTIL010000383         2434 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminobacteriaceae bacterium isolate AMR_MDS_3026
            NODE_51378_length_2434_cov_3.170240, whole genome shotgun sequence.
ACCESSION   DAOTIL010000383 DAOTIL010000000
VERSION     DAOTIL010000383.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868050
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminobacteriaceae bacterium (wastewater metagenome)
  ORGANISM  Aminobacteriaceae bacterium
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae.
REFERENCE   1  (bases 1 to 2434)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2434)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:19:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,224
            CDSs (total)                      :: 3,198
            Genes (coding)                    :: 3,184
            CDSs (with protein)               :: 3,184
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2434
                     /organism="Aminobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_51378_length_2434_cov_3.170240"
                     /isolate="AMR_MDS_3026"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:3034609"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..338
                     /locus_tag="P5201_08400"
     CDS             <1..338
                     /locus_tag="P5201_08400"
                     /inference="COORDINATES: protein motif:HMM:NF012772.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HRV98595.1"
                     /translation="YRERGFRTALDDVGSGYSSLNRLIGLRPDYVKLDIGLVRDIHKE
                     PMKQHVFEAIETICRRSGIEMLAEGIEHAEEVAFFHNAGVRLMQGYYFAKPAEVPQPV
                     SFDDRPSSS"
     gene            412..1305
                     /locus_tag="P5201_08405"
     CDS             412..1305
                     /locus_tag="P5201_08405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006300017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HRV98596.1"
                     /translation="MKEATRAAYEESILRVQMHMQKTLDDPFDANEIASVAGFSRFHL
                     SRLFSAMTGESLLEYRRRLLLERAAHSLLYKARSVTELAFEAGYETPETFTRAFSKLY
                     GVPPGEYRRKLRAAMKHRLEMIQVRSKLFTLSLSIDEGGRKNMEVKIEKHDPLKVAFI
                     RHTGAYDTCGEAWGKLCGAPEVASKLGPDTLALSLCYDDPDVTEPDKIRMDVCVTVDD
                     GFVPGNGIEVQTIPGGEFAVVVYKGAYSGLHDAYRAIYGEWMPKSGREFGDSYSMEIY
                     RTDPEKTPPEESITEIRIPLL"
     gene            1460..1918
                     /locus_tag="P5201_08410"
     CDS             1460..1918
                     /locus_tag="P5201_08410"
                     /inference="COORDINATES: protein motif:HMM:NF016211.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YbaN family protein"
                     /protein_id="HRV98597.1"
                     /translation="MNLWSLLGMMFVLLAFIGALLPVMPTVPFVLVAAACFAKSSPKM
                     HAWLQQHPKFGHIVCDWEEKRCISRGMKIWGISMMTLGGGISVWLVVPRGWPSWTVLG
                     CFIAADMVVLLWKECPSEPGSTRRLMAEGLRKLFFFRGRNGKNSTGKECE"
     gene            1920..>2434
                     /locus_tag="P5201_08415"
     CDS             1920..>2434
                     /locus_tag="P5201_08415"
                     /inference="COORDINATES: protein motif:HMM:NF014234.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004665 - prephenate dehydrogenase (NADP+)
                     activity [Evidence IEA];
                     GO_function: GO:0008977 - prephenate dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_process: GO:0006571 - tyrosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydrogenase/arogenate dehydrogenase
                     family protein"
                     /protein_id="HRV98598.1"
                     /translation="MKKLKECTVSVFGLGLLGASLAWKLTSGGSVQGVAGWSRTRETV
                     QRALDTGIITHACDSAEECAALGDILILAVPIRSMEETSLSIAGAVRQDALAVFDLAS
                     TKTEVGRSLSSIWGPRYAGFHPMAGKEKGGIGNADPELFLGSICAIVPFPGTSPSVLA
                     LAEELATALGGR"
BASE COUNT          484 a          679 c          766 g          505 t
ORIGIN      
        1 actaccgtga aagaggattc aggacggcgc tcgacgacgt cggcagcggc tactcgtcgc
       61 tgaaccgcct tataggtctg cgccccgact acgtgaaact cgacatcgga ctcgtccggg
      121 acatccacaa ggagccgatg aaacagcatg tcttcgaggc catcgaaacg atttgccgca
      181 gaagcggtat cgagatgctc gcggaaggca tcgaacacgc cgaagaggtt gcgttcttcc
      241 acaatgccgg agtgcgcctg atgcaggggt actacttcgc gaaacccgcg gaggttccgc
      301 agcctgtctc ttttgacgat cgaccttctt catcctgaaa aaaatgcgca acgacggtca
      361 ggcggttgcg ttccggatgc ggtatcctct gtgtcacaag gagggggaag aatgaaagaa
      421 gccacgcgcg cggcctacga agagtcgatt ctccgggtac agatgcacat gcagaaaacg
      481 ctggacgatc cgttcgatgc gaatgagatc gcctcggtcg ccggattctc caggtttcat
      541 ctctcgcgcc tcttctcggc gatgaccggc gagtccctgc tggagtaccg gaggcggctg
      601 ctgctggaac gggcggcgca tagcctgctc tacaaggcgc gttcggtgac ggagcttgcc
      661 ttcgaagccg gatacgaaac gccggagaca ttcacaaggg cgttctccaa gctctacggc
      721 gtccccccgg gggagtaccg gcgaaagctg cgggcggcaa tgaagcaccg cctggagatg
      781 atccaggtca ggtcgaagct cttcacactt tccctctcca tcgacgaagg aggaagaaaa
      841 aatatggaag taaaaattga gaagcacgat cctttgaaag ttgcgttcat ccggcacacg
      901 ggggcgtacg acacgtgcgg cgaagcgtgg ggcaaacttt gcggggcgcc tgaagtcgcg
      961 tccaagctgg gtccggatac tcttgccctc tcgctctgct acgacgatcc ggatgtgacg
     1021 gagcccgata agatccggat ggatgtctgc gtcaccgtgg acgacggttt cgtcccgggc
     1081 aacggaatag aagtgcagac catccccgga ggggagttcg ccgtcgtggt ctacaagggg
     1141 gcgtactcgg gtctgcacga cgcgtaccgc gccatctacg gcgaatggat gccgaaaagc
     1201 gggcgcgagt tcggagactc gtactcgatg gagatctacc ggacggatcc cgagaaaacg
     1261 ccgccggagg aaagtatcac ggagatcagg ataccgttgc tgtaacgcct cgcgaaaact
     1321 gatcacaaat cgccggacag cccgctcgcg ccccgcgggt ggtccgtccg gattttttcc
     1381 gtgcttctcc tctcccgttt ttttctattc cgtgctatcg ttgccccgtc ccgtaatcaa
     1441 tttgaactgg tggtgcgcaa tgaatctctg gtctctgctc ggaatgatgt tcgttctcct
     1501 tgcgtttatc ggcgctctgc ttcccgtgat gccgactgtt ccttttgttc tggtcgcggc
     1561 ggcctgtttc gcgaagtcct ccccgaaaat gcacgcatgg ctccagcagc atccgaaatt
     1621 cggccatata gtgtgcgact gggaggagaa gcgctgcatc tccaggggga tgaagatctg
     1681 gggaatcagt atgatgaccc ttggcggcgg aatctccgtg tggcttgtcg tgccccgtgg
     1741 atggccgtcc tggacggttc ttggctgctt catcgctgcc gatatggtcg ttctcctgtg
     1801 gaaagagtgt ccctcggaac ctggatccac gaggcgcctc atggcggagg ggttgcggaa
     1861 actgttcttt tttaggggaa ggaacgggaa aaacagtacg ggaaaggagt gcgagtagca
     1921 tgaagaagct aaaggagtgc accgtctccg tcttcggact cggactgctg ggagcgtcgc
     1981 tcgcatggaa gttgacgtcc ggcgggagcg tgcaaggcgt cgccggctgg agccgcactc
     2041 gggaaaccgt gcagcgtgct ctcgacacag gcatcataac gcatgcctgc gattctgccg
     2101 aggagtgcgc ggcacttggt gatattctga tactcgcagt gcccatccgt tcgatggagg
     2161 agacgagcct aagtatcgcc ggcgcggtgc gtcaggacgc gctcgctgtt tttgatctcg
     2221 ccagtacgaa gaccgaggtg gggcgttcgt tatcatctat ctggggaccg cgatacgcgg
     2281 ggtttcaccc catggcgggg aaggagaagg gggggatcgg caacgccgac cctgagctct
     2341 tccttggttc catctgtgcg atcgtcccct ttccagggac ctccccctct gtccttgccc
     2401 tggcggagga acttgctacg gcgctcgggg ggcg
//
LOCUS       DAOTIL010000384         2433 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminobacteriaceae bacterium isolate AMR_MDS_3026
            NODE_51399_length_2433_cov_3.382254, whole genome shotgun sequence.
ACCESSION   DAOTIL010000384 DAOTIL010000000
VERSION     DAOTIL010000384.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868050
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminobacteriaceae bacterium (wastewater metagenome)
  ORGANISM  Aminobacteriaceae bacterium
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae.
REFERENCE   1  (bases 1 to 2433)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2433)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:19:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,224
            CDSs (total)                      :: 3,198
            Genes (coding)                    :: 3,184
            CDSs (with protein)               :: 3,184
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2433
                     /organism="Aminobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_51399_length_2433_cov_3.382254"
                     /isolate="AMR_MDS_3026"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:3034609"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..205
                     /locus_tag="P5201_08420"
     CDS             <1..205
                     /locus_tag="P5201_08420"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRV98599.1"
                     /translation="LLYGAYFAPALSVATRHSLTLWEWEYICLFYFRLVNTCYLLLEG
                     AMAIVVFLLWRNLRKEEAFDAHS"
     gene            192..596
                     /locus_tag="P5201_08425"
     CDS             192..596
                     /locus_tag="P5201_08425"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRV98600.1"
                     /translation="MLILERFFLWIARWEGIPGFLGSLEVWTNMLCSATDILFVFWFL
                     RIADIARAEQGRKAIRLRYSILAACLLLTPALFLFKGPVLMIWTSLILGIPYILLAGS
                     VFFEIPGIVKLVRRRIHKAPPSGEFSGPDGAL"
     gene            complement(615..1919)
                     /locus_tag="P5201_08430"
     CDS             complement(615..1919)
                     /locus_tag="P5201_08430"
                     /inference="COORDINATES: protein motif:HMM:TIGR04311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM/SPASM family putative metalloenzyme
                     maturase"
                     /protein_id="HRV98601.1"
                     /translation="MNFALPPSERPYPSKLFVEVTTACNLRCAMCVKQSGPGIPDEHM
                     TMSTFQRLVPAFPMLETLLLNGIGEPLLHPRLEAFVQLAKEHLQEKARVGFQTNGTLL
                     TPERARSLLAAGTNIVCLSVDATDPALFQSMREGGETEDVNRAAAALNAARARASSPD
                     FHWGAEIVLTRDTLDELPRVVEWVGERGGAFLLVTHLLPYRQESVPSVAYNPNVDETL
                     AFYREKSREANEQGIDLSRYIEARWRFRPVDNGERTVAFMEKVITEISARGLPQHVPN
                     LVAHDEKQYRRMEELFRRCEELALQHGVELHLPSLSPRNKRRCDFIEEGSAFVTASGT
                     VHPCYFLWHSFTCHADGRIRPVDALSFGSVNETPLLDIWNGPEFSDYRSEIGRYDFPH
                     CGNCSLAPCDYIERHDFEQDCLGNSLTCGSCPWSVGVLQCLR"
     gene            complement(1962..>2433)
                     /locus_tag="P5201_08435"
     CDS             complement(1962..>2433)
                     /locus_tag="P5201_08435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006302022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HRV98602.1"
                     /translation="TFLLIAAILESFLFAFVIMLTVPLSVIGVVPALILTQTPLSIYG
                     LMGVIMLVGLVVNNAIVIVDYAEILRKEGKEPSEAIIEACKVRLRPIIMADATSVIAM
                     VPLAMGLGAGGSYRSPMAIVAIGGLIAGGVLALFAIPPVYKLVWRVKLRLGFNE"
BASE COUNT          513 a          690 c          704 g          526 t
ORIGIN      
        1 gctcctctac ggagcctact ttgccccggc actctcagtc gctacccgtc attcgctgac
       61 tctttgggaa tgggagtata tctgtctctt ctatttccgg ctcgtgaaca catgctatct
      121 ccttctggag ggagccatgg cgattgtcgt atttctgcta tggagaaatc tgcggaagga
      181 ggaggcgttt gatgctcatt cttgaacgtt tttttctgtg gattgcgcga tgggagggca
      241 ttcccggttt tttgggttcg ctggaagtgt ggacgaatat gctctgcagt gcgacggata
      301 ttctcttcgt cttctggttt ctgcgaattg ccgacatcgc ccgtgcagag caggggcgca
      361 aagcaatccg tctccggtat tctattcttg cagcctgttt actgcttacc cccgcgctct
      421 ttttattcaa agggcctgta ctgatgatat ggacgtccct catcctcggc attccgtaca
      481 tactgctcgc cgggagcgtc ttctttgaaa ttccgggcat tgtcaagctc gtgcgccgga
      541 gaattcacaa ggctccgccc tccggagagt tctccggccc ggacggagcc ctgtaaagtg
      601 cggagagaaa aaggttaccg gagacactga agcacgccga cgctccatgg gcacgatccg
      661 caggtaaggg agttccccag gcaatcctgc tcgaaatcgt ggcgctcgat atagtcgcac
      721 ggcgcgaggc tgcagttgcc gcaatggggg aagtcgtacc gtccgatctc gctccggtag
      781 tcggagaact ccgggccgtt ccatatatcc aagagcggcg tttcgttcac cgatccgaag
      841 gagagcgcgt cgacgggcct gattcgaccg tccgcatgac acgtgaagga gtgccacagg
      901 aagtagcagg ggtgcaccgt cccggaggcg gtgacaaacg cgctcccctc ctcgatgaag
      961 tcgcaccgcc gtttgttgcg cgggctaagc gaggggagat gaagttccac tccgtgctgc
     1021 aacgcaagct cctcacatcg gcggaaaagc tcctccatcc gccgatactg cttttcgtcg
     1081 tgcgcgacga ggttggggac gtgctggggc agtccacgcg cagatatttc agtgattacc
     1141 ttctccatga aagcaaccgt tctttcacca ttgtccacgg gacggaatct ccatctggcc
     1201 tcgatgtaac gggagaggtc aatcccctgt tcatttgcct ctctgctctt ctctctgtag
     1261 aaagcaagcg tttcatccac gttggggttg tacgcaacgc ttggtaccga ctcctgcctg
     1321 tatggaagaa ggtgcgttac cagaaggaac gctccccctc gctcccctac ccactcgacc
     1381 acccggggca gctcgtccag tgtgtcccgg gtgagcacga tttcagcccc ccagtggaaa
     1441 tccggggagg aggcgcgtgc acgcgccgcg ttcagggccg cggcggcacg attcacatcc
     1501 tcggtttctc ctccttcccg catgctctga aagagcgccg ggtccgtcgc gtccacggag
     1561 agacagacga tgttggttcc tgcggcaagg agcgagcgcg cacgctccgg agtgagcagc
     1621 gtgccgttcg tctgaaaacc gacgcgagct ttctcctgta gatgttcctt cgccagctgg
     1681 acgaaagcct cgagcctcgg gtgaagcagg ggctcgccga tgccgttgag cagtaatgtc
     1741 tccagcatag ggaatgccgg gacgaggcgt tgaaacgtgc tcatggtcat gtgctcgtcc
     1801 ggtattccgg ggccggactg cttgacgcac attgcgcagc gaagattgca tgcagtggtc
     1861 acctcgacga aaagcttcga ggggtacggt cgttcactcg gcggaagggc aaaattcatt
     1921 ttaaaacctc caaaaaagcc gtgaatggta acctataacg cttattcgtt aaaacctagt
     1981 ctgagcttca ccctccagac cagtttatag actggaggaa tggcgaaaag agcgaggacg
     2041 ccgccggcga tcagacctcc gatggcgacg atggccatgg gagaacggta ggatccgccg
     2101 gcgccgagtc ccatagcaag aggaaccatg gctataaccg atgtggcgtc cgccatgatg
     2161 atcggacgaa gacggacctt gcacgcctca atgatggcct cggacggctc cttcccctcc
     2221 ttgcggagga tttccgcgta atccacgatc acgatggcgt tgttgaccac caaaccgaca
     2281 agcatgatga cacccatcaa accgtagatg gagagcggcg tctgcgtcag aataagcgcc
     2341 ggtacaacgc cgataacgga gagcggaaca gtgagcataa ttacgaaggc gaagagaaac
     2401 gactctaaga tcgccgcaat caaaaggaaa gta
//
LOCUS       DAOTIL010000385         2432 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminobacteriaceae bacterium isolate AMR_MDS_3026
            NODE_51420_length_2432_cov_4.090029, whole genome shotgun sequence.
ACCESSION   DAOTIL010000385 DAOTIL010000000
VERSION     DAOTIL010000385.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868050
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminobacteriaceae bacterium (wastewater metagenome)
  ORGANISM  Aminobacteriaceae bacterium
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae.
REFERENCE   1  (bases 1 to 2432)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2432)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:19:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,224
            CDSs (total)                      :: 3,198
            Genes (coding)                    :: 3,184
            CDSs (with protein)               :: 3,184
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2432
                     /organism="Aminobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_51420_length_2432_cov_4.090029"
                     /isolate="AMR_MDS_3026"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:3034609"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..775
                     /locus_tag="P5201_08440"
     CDS             <1..775
                     /locus_tag="P5201_08440"
                     /inference="COORDINATES: protein motif:HMM:NF014653.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="Ldh family oxidoreductase"
                     /protein_id="HRV98603.1"
                     /translation="ATRRSNHYGIAGYYPLMAIKKGMIGITGTNARPSIAPTFGVENM
                     LGTNPLTWGMPTDEAFPFVLDCATSIAQRGKIEVYDRAGKEIPEGWVIGNDGGIRTDT
                     HQILQDLVSGEAALLPLGGAGEAQAGYKGYGYATVVEILSAALQNGNFLKLLSGFDES
                     GNKHPFGVGHFFIAINVESFIDLSTFKRITGTILRQLRASKKAAGAERIYTAGEKEHL
                     AWLERKDKGAKVDEVLKNQIIELRKEFGLEETYRFPFDE"
     gene            complement(836..2284)
                     /locus_tag="P5201_08445"
     CDS             complement(836..2284)
                     /locus_tag="P5201_08445"
                     /inference="COORDINATES: protein motif:HMM:NF012398.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HRV98604.1"
                     /translation="MEVFSSVDGKKIGELNGASRAQYEEIAAAAHTKFLEWRMVPAPL
                     RGELVRRIGAVLREEKEAIAQVISCDIGKTIIEARGEVQEAIDIADFAVGLSRQLYGR
                     TMHSERPHHRMYEQWHPLGVVGVITSFNFPVAVWAWNALVAAVCGDTILWKPSPKALL
                     SAIKIQSVLEKATDGDNAFRGVFNLCAGSVEEVGEPMLHDARIPLISATGSTAMGRRV
                     GEAVAARFGKTILELGGNNALVITPHARLEHAVRAALFGAVGTSGQRCTTTRRLIVHS
                     SVKDRVTAELIKAYRSLNIGDPLNESTHCGPLVDRESYEAMMLALKNAVWQGGKLLYG
                     GERVLDDHYPEGYYVTPALVEISSDAPVVLTETFAPILYIMEYEDLEDALAIQNNVPQ
                     GLSSALFSDSIREIEAFLSHGGSDCGIANINIGTSGAEIGGAFGGEKETGGGRESGSD
                     AWKGYMRRQTNTINWGDELPLAQGIDFDVSTE"
BASE COUNT          555 a          694 c          649 g          534 t
ORIGIN      
        1 cgccacccgt cgttccaatc actacggtat cgcgggctac tacccgctga tggcgatcaa
       61 aaaaggaatg atcggaatca ccggaacaaa cgcgcgccct tccatcgcgc cgaccttcgg
      121 cgtggaaaat atgctgggta cgaatccctt aacatggggc atgccgacag atgaagcctt
      181 tccgtttgtc ctcgattgcg cgacatccat cgcgcaaaga gggaagatcg aggtttatga
      241 tcgcgcaggg aaagagatcc cggaaggttg ggttatcgga aacgatggag gtatccggac
      301 cgatacgcat cagattttgc aggatctcgt ttccggggag gcggctttgc ttcctctggg
      361 gggcgccgga gaagcgcaag cgggttacaa agggtatggt tatgcgacgg ttgtcgaaat
      421 cttgtctgcc gctcttcaaa acggaaactt cctcaaattg ctcagcggat tcgacgaatc
      481 cgggaataag catcctttcg gtgtcgggca cttttttatc gccattaacg tggaaagctt
      541 catcgaccta tcgacgttca aacgcatcac cgggacgatt ctacgccaac tgcgcgcctc
      601 caaaaaagcg gcgggggcgg agcgtatcta cacggcagga gaaaaagaac acctcgcctg
      661 gctggaaaga aaagacaaag gcgccaaagt ggacgaggtc ctgaaaaacc agatcatcga
      721 gctgagaaaa gagttcgggc tggaagagac gtaccgcttt ccttttgatg aataaagcct
      781 gctgacatca ggacaggaac agggagagaa gagatccttc tctccccttt ttacttcact
      841 ctgttgaaac gtcgaaatcg atcccctggg ccaggggaag ctcgtcaccc cagttgatgg
      901 tattggtctg cctgcgcata tagcctttcc aggcatccga ccctgattca cgtcctcccc
      961 cggtctcttt ctctcccccg aaggctccgc cgatctcggc tcctgacgtt ccgatattga
     1021 tattggcgat accgcagtcc gatcccccat gagagagaaa ggcttcgatc tcccggatgg
     1081 aatcggaaaa gagagccgaa ctcaatccct gagggacatt gttctggatc gccagagcat
     1141 cttcaaggtc ctcgtattcc atgatgtaga ggatcggggc aaaggtctct gtgagaacca
     1201 ccggtgcgtc agaagagatc tccaccagag ccggagtcac gtaatacccc tcaggatagt
     1261 gatcgtccag cacccgctcc cctccgtaaa ggagtttccc tccctgccag acagcgttct
     1321 tcagggcaag catcatagct tcataggact cccggtccac aagggggccg caatgagtgg
     1381 actcgttcag aggatctccg atgttcagcg acctgtatgc tttgatgagt tctgctgtca
     1441 cacggtcttt aacagaggag tggacgatga gcctgcgtgt tgtggtgcat cgctggcctg
     1501 aagtccccac ggctccgaaa agagccgctc tcacagcatg ctccagccgg gcatgaggcg
     1561 taatgaccag ggcattgttc ccgcccagct ccagaatggt cttcccgaaa cgggcggcca
     1621 cggcctcccc cacccggcgc cccatggcgg tgcttcccgt ggcactgatg agagggatcc
     1681 gggcatcatg gagcatgggt tctcccacct cttccacgga tcctgcacac agattgaaga
     1741 ccccgcggaa cgcgttgtct ccgtcggtgg ccttttccag gacactctgg atctttatgg
     1801 ctgagagaag agctttggga ctgggtttcc agaggatagt gtctccgcac acagcagcga
     1861 caagcgcgtt ccaggcccag actgccacgg gaaagttgaa ggaggtgatc acaccgacca
     1921 cacccagggg atgccactgc tcatacatcc tgtgatgagg ccgttcactg tgcatggtcc
     1981 tcccgtacaa ctgccggctc aaaccgaccg cgaaatcagc gatatcgata gcctcctgga
     2041 cctctccccg ggcttcgatg atggtcttcc cgatatcgca agagatgact tgggcgatgg
     2101 cttccttctc ctcacgcaga accgctccga tcctcctgac aagttcaccc cgcaacggtg
     2161 cagggaccat ccgccattcg agaaatttcg tatgagcagc tgcggcgatc tcctcgtact
     2221 gggcacggct tgccccgttg agttctccga ttttttttcc gtcaacggag gaaaagacct
     2281 ccacaagggc tccttttccc gcttccccgg accctgaggt cccgtaatgc agagcctcgt
     2341 ctttgatatt ttgatttttg atgtgaaaca tcatgactcc tttcgatatg tttatataaa
     2401 catatcgtct gaaagagcga tgtcaatatg aa
//
LOCUS       DAOTIL010000386         2427 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminobacteriaceae bacterium isolate AMR_MDS_3026
            NODE_51589_length_2427_cov_3.470067, whole genome shotgun sequence.
ACCESSION   DAOTIL010000386 DAOTIL010000000
VERSION     DAOTIL010000386.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868050
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminobacteriaceae bacterium (wastewater metagenome)
  ORGANISM  Aminobacteriaceae bacterium
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae.
REFERENCE   1  (bases 1 to 2427)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2427)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:19:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,224
            CDSs (total)                      :: 3,198
            Genes (coding)                    :: 3,184
            CDSs (with protein)               :: 3,184
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2427
                     /organism="Aminobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_51589_length_2427_cov_3.470067"
                     /isolate="AMR_MDS_3026"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:3034609"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..342
                     /locus_tag="P5201_08450"
     CDS             <1..342
                     /locus_tag="P5201_08450"
                     /inference="COORDINATES: protein motif:HMM:NF019843.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HRV98605.1"
                     /translation="PKDSMKAFYWFDRAARNGYIDAMVNLGVLYEKGDGAVFSPEKAI
                     RWYTEAAEKGSFLGMAYLGEAYYQGRGVRKDEKKAVGLLEQAASSGLPYAQRLLYRID
                     RIKYMRYRDVQ"
     gene            complement(518..1153)
                     /locus_tag="P5201_08455"
     CDS             complement(518..1153)
                     /locus_tag="P5201_08455"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRV98606.1"
                     /translation="MRVQTSDVLKFLFLYVLFAASVSSPALCFDDSILPPLPEDELAQ
                     PHPQEDLPESGDVSGLDPSEVKTTGGVLAGVSSDIFLPPLDADPEARARAVAIWNGAA
                     ELQRGKKYEEALEKYREGLAVCEDPVVREHIAKLEAFLELQRKKAEASSARARAVALW
                     NEAAELQLAKKYEEALEKYREGLAIASDPVVEEHVKKLREFIEKRKARGKQ"
     gene            complement(1228..1995)
                     /locus_tag="P5201_08460"
     CDS             complement(1228..1995)
                     /locus_tag="P5201_08460"
                     /inference="COORDINATES: protein motif:HMM:NF019844.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0070064 - proline-rich region binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SH3 domain-containing protein"
                     /protein_id="HRV98607.1"
                     /translation="MKVFRYSVCAALIVCLCLALAVPVLADPAEMLLAKITGTNVNVR
                     AEPSRTGRLTGRFTGGEHAVVLEERSGADPFPWSRVIAFASGGTLVEGWVYGQYVEPL
                     PQDDWSNSAEGDPGERFSRFFDKTLERIGDTPEEAAARFGAPAAREDTRLPGRHDPAY
                     MVDYFRLEYPAFELLYFKTKDSYGLIGVKIVEGEAVIGEGIRLGADAASVVRELGVPL
                     YQKGSAFAWTDEPGYGVLTLTFAEGRVLNIDLDVELD"
     gene            complement(2038..>2427)
                     /locus_tag="P5201_08465"
     CDS             complement(2038..>2427)
                     /locus_tag="P5201_08465"
                     /inference="COORDINATES: protein motif:HMM:NF019349.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FCD domain-containing protein"
                     /protein_id="HRV98608.1"
                     /translation="ARVALPFIDDAALADLRRKVDDMGTFTVAGDTARLVAANKEFHR
                     KLYSMGPFPMLNDMIFKLWDGGAFSSIVFALRPDRIRTVYEEHLAILAAIERRDAEEL
                     ERLVRAHKSGFAEALCEISARKRLETL"
BASE COUNT          524 a          758 c          684 g          461 t
ORIGIN      
        1 cctaaggact cgatgaaggc cttctactgg ttcgacagag ctgcccggaa cgggtacatc
       61 gacgccatgg tcaatctagg ggtgctctac gagaaaggcg acggcgccgt cttcagcccg
      121 gagaaagcga tccgctggta cacggaagcg gcggagaagg ggtctttcct tggtatggcg
      181 tacctcggag aggcctacta ccagggcagg ggcgtcagaa aagacgaaaa gaaagcggtg
      241 gggctcctcg aacaggcggc ctcgtcgggg ctgccctacg cgcagagact gctctaccgg
      301 atcgacagga tcaagtacat gcggtaccgt gacgttcaat agcccgacat acacgctgtc
      361 gccggaagcg tggaagtctt tcggcgctcg accgtagtca taaagacctc atccccttca
      421 acgggatgag gtctcttctt ccgcacacgg aacacttccg ccggagcggc gtttcggaga
      481 gcattcgata gaattgtttc atcggggcgc tccttgttta ctgctttccg cgggccttcc
      541 gcttctcgat gaactcccgg agcttcttga catgctcctc gacgacaggg tcggatgcta
      601 tggcgagtcc ctcgcggtac ttctccagcg cctcttcgta cttctttgcg agctgcaatt
      661 cagcggcttc gttccacaac gcaacggcgc gcgcacgtgc cgaggaggct tccgcctttt
      721 tccgctgaag ctcaagaaat gcctccagct ttgctatgtg ctcgcggaca acgggatcct
      781 cgcacactgc aagcccctcg cggtacttct ccagcgcctc ttcgtacttt ttcccacgct
      841 gcaactccgc agcgccgttc caaattgcaa cggcgcgagc acgggcttcc gggtccgcgt
      901 ccagcggggg taaaaaaatg tccgacgata ctcctgcgag aacgcctccc gtcgtcttca
      961 cctcggaagg gtcgagtccg gaaacatcgc cgctctcagg gaggtcttcc tgaggatgag
     1021 gctgagcgag ttcatcctcg ggaagcggcg gtaaaatact gtcgtcgaag cagagcgcag
     1081 gagaagatac gctcgccgca aacagcacgt acaggaacaa aaacttcaga acatcggagg
     1141 tctgtactct catggcaaaa aacacctcca gaaaaaaagt atcgacgcat acgcgctctc
     1201 ccgagaaaaa gcgccccgcc atgcctccta atccagttcg acgtccaggt cgatattgag
     1261 aacgcgcccc tccgcgaagg taagtgtgag gacgccgtac ccgggctcgt cggtccatgc
     1321 gaaggcagag cctttctgat acaacgggac gccgagttcc cggaccacgg aagcggcgtc
     1381 ggcgccgaga cgaatgccct cgccgatcac ggcctcgccc tcgacgatct tcactcctat
     1441 cagaccgtaa ctgtctttcg tcttgaaata gagaagttcg aaggccgggt actcaaggcg
     1501 gaaatagtcc accatgtagg cggggtcgtg ccggccggga agcctggtat cctcacgcgc
     1561 agcaggagcg ccgaacctgg cggccgcctc ctccggagta tccccgatgc gttccagggt
     1621 tttatcaaaa aaacgtgaaa agcgttcgcc gggatctccc tcggcgctat tgctccagtc
     1681 gtcctggggc agcggttcaa cgtactggcc gtagacccag ccctcaacga gcgtccctcc
     1741 ggaagcgaat gcgatgaccc gcgaccacgg gaaggggtcc gcgccggagc gttcttcgag
     1801 gacaaccgca tgctcgcccc ctgtaaaacg ccccgtgaga cggcctgtcc gggatggttc
     1861 ggctcgcaca ttcacattcg tcccggtaat tttcgccagg agcatctccg ccgggtcggc
     1921 caagacgggc acggcaagcg ccagacacag gcacacaatc aacgccgcac atactgaata
     1981 tcgaaaaacc ttcaccaatt ccaactcctt tcgggatgaa agtaccggat actgccgcta
     2041 caaagtctcg agccgctttc tcgcagaaat ctcacaaagc gcctcggcaa aaccgctctt
     2101 gtgcgcccgt accaggcgtt ccagttcctc tgcgtcgcgc cgctctatcg cggcaaggat
     2161 tgcaaggtgc tcttcgtaca ctgtcctgat acggtcgggt ctgagagcga agacaatgct
     2221 cgaaaaggcg cccccgtccc agagtttgaa gatcatgtcg ttcagcatcg ggaaggggcc
     2281 catggaatag agcttccgat ggaactcctt gttcgccgca accaggcgag ccgtatcgcc
     2341 ggcgaccgta aacgttccca tatcatccac cttgcggcgc aaatccgcaa gcgccgcgtc
     2401 gtcgatgaat ggaagggcga cccgggc
//
LOCUS       DAOTIL010000387         2425 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminobacteriaceae bacterium isolate AMR_MDS_3026
            NODE_51657_length_2425_cov_1.989873, whole genome shotgun sequence.
ACCESSION   DAOTIL010000387 DAOTIL010000000
VERSION     DAOTIL010000387.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868050
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminobacteriaceae bacterium (wastewater metagenome)
  ORGANISM  Aminobacteriaceae bacterium
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae.
REFERENCE   1  (bases 1 to 2425)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2425)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:19:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,224
            CDSs (total)                      :: 3,198
            Genes (coding)                    :: 3,184
            CDSs (with protein)               :: 3,184
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2425
                     /organism="Aminobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_51657_length_2425_cov_1.989873"
                     /isolate="AMR_MDS_3026"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:3034609"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            169..519
                     /locus_tag="P5201_08470"
     CDS             169..519
                     /locus_tag="P5201_08470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005661144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043856 - anti-sigma factor antagonist
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="STAS domain-containing protein"
                     /protein_id="HRV98609.1"
                     /translation="MFEMKITVESWGRDVSLTGRLDAGSAPDFEKEAIASVDAAGESV
                     HFVLDFTNLEYISSAGLRSILVLAKRLAPSGGKVVLCGMSGVVEEVIGVSGFDSFLPV
                     AGSRGDALRIVGAV"
     gene            534..962
                     /locus_tag="P5201_08475"
     CDS             534..962
                     /locus_tag="P5201_08475"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006299637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HRV98610.1"
                     /translation="MESTVRERTFPASLDCLGGFRAFILEENGAEERLPPKRVAHLDV
                     AIEEIVVNICSYAYETPPGKLTIRVEESPEAFAVEFLDNGVAFDPLEVDEPDVTRPLE
                     ERDAGGLGILLVRRMMDEVHYSRKETLNSLRIVVKTPRER"
     gene            976..1992
                     /locus_tag="P5201_08480"
     CDS             976..1992
                     /locus_tag="P5201_08480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006299638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HRV98611.1"
                     /translation="MFPGSLAARRLPAFLLFVLLLLFFAGGAQGQQAAPPLTKATLTL
                     QWYPQTQFAGYYMALEKGFYAARGIDLEIRRGGSDVDAIGDLRTGRTDFATLFLTAGI
                     RYRSKDLPLVNVGQIVNRGNLLIVAYKNRGIRTVRDLNGRKMSLWGDHFSGGYKKLFS
                     DNGIWPVIFPQLISVEVFLRGGVDACCAMSYNEYIRILHAGRRPEDLVVISLREEGYD
                     FPEDGIYCMEETLLRNPRLARDMREASLEGWRYAAEHPDEAVDLILRKADEAGYIVNR
                     LLLRRMLDTILPSIFPEDSSEWTPGVLSRTDYERTFDMMETVFVEMTERVPYSVFFPG
                     SGER"
     gene            1989..>2425
                     /locus_tag="P5201_08485"
     CDS             1989..>2425
                     /locus_tag="P5201_08485"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRV98612.1"
                     /translation="MKEHLFPLFPDGRRGVSIAAKLSLMIIAGVGAIFLAIVFYGYST
                     SRAVLEEEFSARMENLGNAAESKFRQIPRVAEAVTRDLVSVLTFFNPPPGKIPEMMKV
                     LLESHPEVSGLSIGFSKQAGDPLLRNFCPIVFRRGGAILYQDLA"
BASE COUNT          446 a          695 c          753 g          531 t
ORIGIN      
        1 ggagaagccg gttatagaga gggagaagga aatttcgttg gaactgctcc gacatggtct
       61 ctccctgaag ttttcttgac acgtccggcg aaaacactca caatggccgc atatggattt
      121 tagcgtcaat attagcggct tcggccgctc tccctggggg aatgaacgat gttcgaaatg
      181 aaaattacgg tcgagtcctg ggggcgggat gtctcactca cagggcgcct ggacgccgga
      241 tcagcgcccg attttgaaaa ggaagccata gcttctgtcg atgcggccgg ggagagtgtc
      301 cactttgtcc tcgacttcac gaatctcgaa tatatcagca gtgcaggtct gcgctccatt
      361 ctcgtccttg cgaagcgcct tgctccttcc ggcggcaagg tggttctctg cggcatgtcc
      421 ggtgtcgttg aagaagtcat cggtgtctca ggcttcgaca gctttcttcc cgtggccggt
      481 agccgcgggg atgccctccg aatcgtcgga gcggtgtaga gggggagatg tgaatggaat
      541 ccactgtcag agaacggact tttccagcct ctcttgattg cctgggcggc ttccgggcct
      601 tcatcctgga ggaaaacgga gcagaggagc gtcttccccc gaagcgcgtt gcgcacctgg
      661 atgtcgcgat tgaggagatc gtggtgaaca tctgttccta cgcctacgag actcctcccg
      721 gaaaactcac catcagggtg gaagagtctc ccgaggcttt cgccgtcgaa tttctggaca
      781 acggcgtggc gttcgatccc ctcgaggtgg acgagccgga tgttacccgt ccgcttgaag
      841 agcgtgatgc cggcggcctg ggcatcctcc tggtccggag gatgatggac gaggtccact
      901 actcccggaa ggagacgctg aacagtctcc gtattgtggt gaagacgccc cgggaaaggt
      961 agggaggcgt ctcaggtgtt ccccggctct ttggccgccc gtcgcctgcc ggctttcctt
     1021 ctttttgtcc ttctcctcct cttcttcgcc ggaggagcgc aggggcagca agcggctccc
     1081 cccctgacga aggcgaccct gacccttcag tggtatcccc agacacagtt cgcggggtat
     1141 tacatggcgc tggagaaggg gttttatgct gcccgcggca tcgacctcga aatccggcgc
     1201 ggaggctccg acgtcgatgc cattggggat ctccgcaccg gaaggaccga cttcgcgacg
     1261 ctcttcctca ctgcgggcat ccggtatcga tcgaaggatc tgcccctggt gaacgtcggg
     1321 cagatcgtca acaggggaaa tctgcttatc gtggcgtaca aaaaccgcgg catccgcacg
     1381 gtcagggatc tgaacgggcg caagatgagc ctgtggggag accatttttc aggcggatac
     1441 aagaaactct tcagcgacaa tggaatctgg ccggtgatct tcccccagct catatcggtg
     1501 gaggtcttcc tgcgcggggg agtggacgcc tgctgcgcca tgagctacaa cgagtatatc
     1561 cgcattctcc atgccggacg gcgccccgaa gatctcgtgg tgatctctct ccgggaggag
     1621 gggtacgact ttccggaaga cggtatttac tgcatggaag agacattgct caggaatccg
     1681 cgccttgccc gggatatgcg cgaggcctcc ctggaggggt ggcgctacgc ggccgaacat
     1741 cccgacgagg ccgtcgacct gatcctccgc aaggccgatg aggcagggta catcgtgaac
     1801 cgtcttctgc tgcgccggat gctcgacacg atccttccgt cgatctttcc ggaggactcc
     1861 tcggagtgga ctcccggggt gctctccagg acagactatg agcgcacctt cgacatgatg
     1921 gagactgtct tcgtcgagat gacggagcgg gttccctata gcgttttctt tccggggagc
     1981 ggcgagcgat gaaggagcat ctttttcccc tctttcccga cggccgcagg ggggtctcca
     2041 tcgcggccaa gctttccctg atgatcatcg cgggcgtcgg cgcgatcttt ctcgcgattg
     2101 tcttctacgg ctactcgaca tcgagggcgg tcctggagga ggagttcagc gcacggatgg
     2161 agaacctcgg caacgccgct gagagcaagt ttcgccagat ccctcgcgtg gccgaggcgg
     2221 tgacgcggga tcttgtctcc gtgctgacct ttttcaatcc tcctcccggg aagattcccg
     2281 aaatgatgaa ggtgcttctg gagtctcatc ccgaggtctc cggactctcg ataggtttct
     2341 cgaagcaggc gggggatccg ctgctgcgga acttctgccc tatcgtcttc cggagagggg
     2401 gggcaattct ctaccaggat ctcgc
//
LOCUS       DAOTIL010000388         2421 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminobacteriaceae bacterium isolate AMR_MDS_3026
            NODE_51742_length_2421_cov_4.250634, whole genome shotgun sequence.
ACCESSION   DAOTIL010000388 DAOTIL010000000
VERSION     DAOTIL010000388.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868050
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminobacteriaceae bacterium (wastewater metagenome)
  ORGANISM  Aminobacteriaceae bacterium
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae.
REFERENCE   1  (bases 1 to 2421)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2421)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:19:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,224
            CDSs (total)                      :: 3,198
            Genes (coding)                    :: 3,184
            CDSs (with protein)               :: 3,184
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2421
                     /organism="Aminobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_51742_length_2421_cov_4.250634"
                     /isolate="AMR_MDS_3026"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:3034609"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            18..218
                     /locus_tag="P5201_08490"
     CDS             18..218
                     /locus_tag="P5201_08490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013049149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0001072 - transcription antitermination
                     factor activity, RNA binding [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HRV98613.1"
                     /translation="MSQGTVKWFNDSKGYGFITTDEGKDVFVHFSAITGDGFKTLAEG
                     QKVSFEVVDGEKGPQAANVHKA"
     gene            complement(279..2006)
                     /locus_tag="P5201_08495"
     CDS             complement(279..2006)
                     /locus_tag="P5201_08495"
                     /inference="COORDINATES: protein motif:HMM:NF012492.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HRV98614.1"
                     /translation="MSENGFACYSLREELLLALNKKGFSIPTPVQEKVLSMDRFDTDL
                     IVRAKTGSGKTLAFLLPLLQEMELPASAPLILVLSPTRELAQQTAREAEWLSRYLDIS
                     VATLVGGLDMTTQIRSLKEGAVVVVGTPGRTLDHVNRGSLKTEKIHTVILDEGDHMLD
                     MGFRDELEGILDALPHRNRTWLFSATMPREVKELSRKYLSSPVSISLVEDGDQHEDIV
                     HKAYLVPFRHKMEGLVNVLLWERPKRGLIFCHTRLETMNVSQRLNEEGFNSGSLHGEM
                     TQRERNAVLSAFKNGHLPLLVATNVAARGLDVEGVTHVIQIGLPDDRDTFVHRSGRTG
                     RAGHEGMDILLLSPQEAEKFKYMIGSAKVNIEWNNVPDVQAIGKVQREIAEEKLLTLK
                     EASENEDYLQWAEDLLTRVNPKNLVARLLFSLTSKRSAGYNLAADLERELRRKSRGPV
                     EKDRTPGNRSLKGGRPKGTMLRLSKGLADGWDVGRVLHSVCTSLNVDRSEVGAIRMKD
                     DHVLVELLPVALSRFEEDSRGLERCGLIEEGKGRDMLFQAYSTSRPSRDRAPSPRPRR
                     QSKTDSRNR"
     gene            complement(2221..>2421)
                     /locus_tag="P5201_08500"
     CDS             complement(2221..>2421)
                     /locus_tag="P5201_08500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014163875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HRV98615.1"
                     /translation="TVSKATIGQALLGTVLFHALFIVSPLAGKNLMGSAQVGEYFRVF
                     IAYGVIGIALALHAWQRLVKKQ"
BASE COUNT          550 a          609 c          670 g          592 t
ORIGIN      
        1 taggaggctg tctttggatg agtcagggaa ctgtgaaatg gtttaacgat agcaaggggt
       61 atggtttcat tacgacggac gagggcaaag atgttttcgt tcatttcagc gcaatcacag
      121 gcgatggttt taagacgctt gccgagggcc agaaggtaag ttttgaggta gtggacggcg
      181 aaaagggccc tcaggccgct aacgttcata aggcgtaatt attccccgat tataataata
      241 ttagataaag ccggcctcaa acgaggccgg ctttatactt atctatttct ggaatctgtt
      301 ttactctgtc ttctgggtct gggagaaggg gcgcggtctc tcgatggtct cgaagtcgag
      361 tacgcctgga agagcatatc tctccctttt ccctcttcga taagtccgca acgttcaagg
      421 ccgcgagaat cctcttcaaa acgggaaaga gccactggaa gtagttccac caggacatgg
      481 tcgtctttca ttcggatagc gccgacttcc gagcggtcaa cgttaagcga cgtacacacg
      541 gaatgcagca cccgtcccac atcccatccg tcggcaagtc ccttgctcaa gcgaagcata
      601 gttcccttgg ggcggccccc ttttaaagaa cgattgccgg gagtgcggtc tttttcaacg
      661 gggccgcggc tttttcttcg aagctctctt tcaagatcgg cggcgagatt gtatccagca
      721 gagcgcttgg atgtcagtga aaagagaaga cgggcgacga ggtttttagg attcaccctt
      781 gtcagcaggt cttcggccca ctgaaggtag tcttcatttt cactggcttc tttcagcgta
      841 agcagttttt cttccgctat ctctcgctgt accttgccga tagcctgcac gtctgggacg
      901 ttgttccatt cgatgttcac cttggcggag ccgatcatgt acttgaactt ttccgcttcc
      961 tgcggagaga gaagaaggat atccatacct tcgtgtcctg ctctgcctgt acgtccgctg
     1021 cggtggacga aggtatccct gtcgtcggga aggccgatct gtatgacgtg ggtcactccc
     1081 tcgacgtcca ggccgcgcgc cgctacattt gttgcgacca gaaggggaag atggccgttc
     1141 ttgaaggctg aaaggacggc gttccgctct ctctgggtca tttctccatg gagcgagccg
     1201 gaattgaagc cctcctcgtt aagacgctga gagacgttca tcgtttcaag acgggtatgg
     1261 cagaagatca gtccccgctt cgggcgctcc caaagaagaa cgttcaccaa tccctccatc
     1321 ttatgccgga atggaaccag gtaggccttg tggacaatat cctcgtgctg atcgccgtct
     1381 tcaactaaag agatggaaac ggggctggag aggtactttc tggagagctc cttgacctcc
     1441 ctcggcatag tagcggagaa aagccacgtc cggttacgat gggggagcgc gtccagaatg
     1501 ccttcaagtt cgtcgcggaa tcccatgtcg agcatgtgat cgccctcgtc caggatgaca
     1561 gtgtggattt tctccgtctt cagcgaaccg cggttgacat gatcaagcgt tcttccggga
     1621 gtcccgacga cgacgactgc gccttctttg agcgaacgga tttgagtcgt catatccagc
     1681 ccgccgacga gagtggcgac ggaaatatcg aggtaacgtg aaagccactc cgcctcacga
     1741 gctgtttgct gtgcaagctc acgggtgggt gaaagcacca ggatgagagg ggctgatgcg
     1801 ggaagctcca tttcttggag cagagggagc aggaacgcca gcgttttgcc cgatcccgtc
     1861 tttgcccgga cgatcaggtc ggtatcgaaa cggtccatcg aaagcacttt ttcctggacc
     1921 ggtgtgggga ttgaaaagcc tttcttgttg agcgcgagaa gaagttcttc ccgcagagaa
     1981 tagcacgcga aaccgttttc actcatgagt atattattcc tcctcaaatt aggagggcaa
     2041 atcctgggga ataatgcatc aaatagtact tgccgttctg tgttcggccc gcgttcccca
     2101 aaaaagagtg gaccccgttc cgggggtcca ctcaaattct tccctccatt gacaaactga
     2161 aattatacat gaatttcaga atatgtcaat atggaggcgc gaatctgtgt gacgagaatc
     2221 ttactgtttt tttaccaatc gttgccaggc gtgaagcgcc agagcgatgc caatgacgcc
     2281 gtaggcaatg aaaacacgga agtattcgcc tacctgtgcg cttcccataa gatttttccc
     2341 ggcaagtggg gagacgatga aaagggcgtg aaagagtacg gttccaagaa gcgcctgacc
     2401 tatagtggct ttcgagacgg t
//
LOCUS       DAOTIL010000389         2419 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminobacteriaceae bacterium isolate AMR_MDS_3026
            NODE_51805_length_2419_cov_4.121827, whole genome shotgun sequence.
ACCESSION   DAOTIL010000389 DAOTIL010000000
VERSION     DAOTIL010000389.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868050
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminobacteriaceae bacterium (wastewater metagenome)
  ORGANISM  Aminobacteriaceae bacterium
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae.
REFERENCE   1  (bases 1 to 2419)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2419)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:19:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,224
            CDSs (total)                      :: 3,198
            Genes (coding)                    :: 3,184
            CDSs (with protein)               :: 3,184
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2419
                     /organism="Aminobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_51805_length_2419_cov_4.121827"
                     /isolate="AMR_MDS_3026"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:3034609"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1294
                     /gene="glgP"
                     /locus_tag="P5201_08505"
     CDS             <1..1294
                     /gene="glgP"
                     /locus_tag="P5201_08505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013047645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="alpha-glucan family phosphorylase"
                     /protein_id="HRV98616.1"
                     /translation="RKLSWQLYGGDQLYRLCQEMVLGVGGLRMLRDLGYKNIETFHMN
                     EGHAGFLTLELMREQGYFDIEKIREQVIFTTHTPVPAGHDFFRFDLVDKVISQEALSN
                     LKRMLPNSDGVSMTELGIRFSRYVNAVSKKHAEVSRNMFTMPEIDWVTNGVHPGTWVS
                     TGMRRLFNTHIPGWENDPGRLVQAVKIPPEDIWSAHQASKLRLLAGIMEQTGQQFEPD
                     ILTIGFARRAATYKRADLLFSNMKRLLEVTSGKIQLVFAGKAHPKDDGGKAVLQRLLS
                     ASQKVGEMIPIVFLENYDMEKGAMITQGVDLWLNTPLRPREASGTSGMKCTLNGVMNF
                     SVLDGWWIEGWIEDVTGWSIGPDPTESEMVHYDESLDANDLYDKLENKIIPTYYNNRE
                     KWIWMMQRTIAMNGSFFNTHRVVREYCEKAYNISFRGM"
     gene            1301..>2419
                     /locus_tag="P5201_08510"
     CDS             1301..>2419
                     /locus_tag="P5201_08510"
                     /inference="COORDINATES: protein motif:HMM:TIGR02095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen/starch synthase"
                     /protein_id="HRV98617.1"
                     /translation="MATLHASREIKVLHVSPEAVPFCKVGGLADVAGSLPPSLRKSGT
                     DCRLLLPAWDGVLDTAHKRGLKLVRLPRSVDVAIRWKVYNGTLWMSSGEDFPVYFLES
                     PFFEKKQIYPQQLDPESVLPFVFLSLAALELADATEWDADVLHCHDWGTAPLISALLW
                     HRYYRFMFSQYKTVFTIHNLAHQGLLPLHSLRDWGLVDDAFTLNGLEFYGGANLMKGA
                     ITGASAVTTVSPTYSHEIQTENGGVGLGGLLRSQNSKIRGILNGLDTEYWNPATDSLL
                     FQNYSPDDLSGKTRCRNALLDRLGWKEDGRPLFTSVGRMVDQKGFDILLPAIPRMVEW
                     GCRLFLVGSGQREYETAIQEAAAKWPDAVASFIGYDEEI"
BASE COUNT          544 a          697 c          682 g          496 t
ORIGIN      
        1 ccgcaaactc tcgtggcagc tctacggagg ggaccagctc taccgcctct gccaggagat
       61 ggtgctcggc gtgggcggtc tccgcatgct ccgcgacctc gggtacaaaa acatcgagac
      121 gttccacatg aacgagggac acgccggttt cctcaccctc gaactcatga gggaacaagg
      181 ctacttcgac atcgaaaaga tccgtgaaca ggtcatcttc acgacgcata ccccggttcc
      241 ggcagggcac gacttcttcc gtttcgacct tgtggacaag gtcatctcgc aagaggcgct
      301 ctccaatctc aagaggatgc tcccgaactc ggacggcgtt tccatgaccg aactgggcat
      361 ccgtttcagc cgctacgtca acgccgtctc gaaaaaacac gccgaggtca gccggaacat
      421 gttcaccatg ccggagatag actgggtcac caacggcgtg catccgggca catgggtgag
      481 cactggtatg cgcaggctct tcaacacgca tattccgggc tgggagaatg atcccggacg
      541 gttagtccag gcagtgaaga tccctcccga ggatatctgg agcgcgcacc aggcgtcgaa
      601 actgcgcctc ctcgccggaa tcatggaaca aaccggacag cagttcgaac cggatattct
      661 gaccataggc tttgcacgga gagccgcgac gtacaaacgt gcggatcttc ttttttccaa
      721 tatgaaacgc cttcttgaag tgacctcagg caaaatacag ctcgtcttcg cagggaaagc
      781 gcacccgaag gacgacggag gcaaggctgt tctccagcgt cttctgagcg cttcccagaa
      841 ggttggagag atgatcccca tcgtcttcct tgagaactac gacatggaaa agggagcgat
      901 gatcacacag ggcgtcgatc tttggctgaa taccccgctc cgcccgcggg aagcgtccgg
      961 aacaagcggc atgaagtgca cgctgaacgg ggtcatgaac ttttccgtcc tcgacggatg
     1021 gtggatagaa gggtggatcg aggacgtcac cggatggtcg ataggccccg atcccacgga
     1081 gtcggagatg gtccactacg acgaatcgct ggacgcaaac gacctctatg acaagctgga
     1141 gaacaaaatc attccgacct actacaacaa ccgcgaaaaa tggatctgga tgatgcaacg
     1201 gaccattgcc atgaacggga gctttttcaa tacgcatcgc gtggtccgcg agtactgcga
     1261 aaaggcgtac aacatcagtt tccgggggat gtgatccgca gtggcgacac ttcatgcttc
     1321 aagagaaatc aaggttcttc acgtaagccc cgaagccgtc ccgttctgta aggtgggagg
     1381 gctggcggac gttgccggct cgctgccccc ttccctccga aagagcggta cggactgccg
     1441 cctgcttctt cccgcgtggg acggcgttct tgatactgcg cacaagcgcg gcctgaaact
     1501 cgtccggctt cctcggagcg tcgatgtcgc catccgctgg aaggtgtaca atggcacgct
     1561 gtggatgtcc tccggcgagg atttccccgt ctattttctc gagtctccgt tcttcgagaa
     1621 gaaacagatc tatccgcagc agcttgaccc ggagtccgtg cttccgtttg tctttctttc
     1681 cctggcggcg cttgaactcg ctgatgccac ggaatgggat gccgacgtgc tgcactgtca
     1741 cgactgggga acagccccgc tgatcagcgc gctgctctgg cacaggtact acaggttcat
     1801 gttttcgcag tacaagacag tcttcaccat acataatctt gcgcatcagg gccttctccc
     1861 tctccactcg ctccgcgact ggggacttgt cgacgacgct tttacgctca acggattgga
     1921 gttctacggg ggcgcaaacc tcatgaaggg cgcgatcaca ggcgcttccg ctgtaactac
     1981 cgtcagcccg acctattctc atgagatcca gaccgagaac ggaggggtcg gtctcggggg
     2041 gcttctcagg agtcaaaact caaagatacg cggcattctc aacggcctgg atactgagta
     2101 ctggaacccg gccacagaca gcctcctttt tcaaaactat tctccggatg atctctcggg
     2161 gaaaacccga tgccggaatg cccttcttga ccggctcgga tggaaagaag acggtcggcc
     2221 gctctttacc tcggtcggca ggatggtaga tcaaaagggg ttcgatattc tcttgcccgc
     2281 gatcccgagg atggtcgaat gggggtgcag actctttctt gtcgggagcg ggcaacgtga
     2341 atacgagacc gcaattcagg aagccgccgc aaaatggccg gacgctgttg cctcatttat
     2401 aggatacgac gaagagata
//
LOCUS       DAOTIL010000390         2412 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminobacteriaceae bacterium isolate AMR_MDS_3026
            NODE_52009_length_2412_cov_3.809928, whole genome shotgun sequence.
ACCESSION   DAOTIL010000390 DAOTIL010000000
VERSION     DAOTIL010000390.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868050
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminobacteriaceae bacterium (wastewater metagenome)
  ORGANISM  Aminobacteriaceae bacterium
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae.
REFERENCE   1  (bases 1 to 2412)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2412)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:19:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,224
            CDSs (total)                      :: 3,198
            Genes (coding)                    :: 3,184
            CDSs (with protein)               :: 3,184
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2412
                     /organism="Aminobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_52009_length_2412_cov_3.809928"
                     /isolate="AMR_MDS_3026"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:3034609"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            8..1054
                     /gene="selD"
                     /locus_tag="P5201_08515"
     CDS             8..1054
                     /gene="selD"
                     /locus_tag="P5201_08515"
                     /EC_number="2.7.9.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006300875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004756 - selenide, water dikinase
                     activity [Evidence IEA];
                     GO_process: GO:0001887 - selenium compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_except=(pos:41..43,aa:Sec)
                     /transl_table=11
                     /product="selenide, water dikinase SelD"
                     /protein_id="HRV98618.1"
                     /translation="MRLTEMSTTSGUAAKIGPADLSKILKNIPLPSDERIFSSWASGE
                     DAALWKIDEKRAGILTVDFITPVVDDPLVWGRIAAANSLSDVFAMGGFPLVALNIVGF
                     PVKSLDMSVLEAVLAGGFEKVREAGAFLLGGHSVEDKEPKYGLVAYGEVAMDSVWRVT
                     GACPGDMLLLTKPIGTGIVATALKADMLEDERSLQEAIRWMSMLNDLPRRLDTGLLTS
                     VRACTDVTGFGLAGHALDMLSSKEMNLVLGIHDIPLLPGTISLAESGLIPAGTYNNRI
                     EYEDRVRDPQKHDEVLMDMLFDAQTSGGLLLALPEEKVQDFLKAVQGIGFELSSVIGR
                     FTKGNGELEIVDNI"
     gene            1123..2295
                     /locus_tag="P5201_08520"
     CDS             1123..2295
                     /locus_tag="P5201_08520"
                     /inference="COORDINATES: protein motif:HMM:NF018485.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HRV98619.1"
                     /translation="MLSQKKSRRVRFVLSLSMFCAMLGIGIIAPVIPLYAKSLGASSV
                     AIGFIIGSFSFSRIWGMLVSGELAERMDRKKLLLGGLFVYAAASVAYTFSFSTEMLIF
                     IRTLHGIGSAMVVPITMAIAADIAPKGEEGAIFGSLQAALFLGVGSGPLLSGFLADTF
                     GFYAPFYAMTALTLLALFLVLALFPSSPPKNTVKEEVGALKAFKGIISDPEMLNVFIF
                     QFSSAMCRGVLVMVIPLFASQFDLSLSRIGIIVSLNSISTGILQNVSGKMADNVHKNT
                     LIIIGGVISAVTLIGLPNLTTPLSLSVASVAFGVGHAFASPSLAAIAAARGKNFGSGR
                     TMGLFNIAFSIGMTIGPIMVGSLLDITGNGIPFYILSAFLAASALPFFPVFKRGVC"
BASE COUNT          528 a          531 c          645 g          708 t
ORIGIN      
        1 agatggaatg cgtttgactg agatgtccac tacaagcggg tgagctgcta aaataggtcc
       61 ggcggacctt tctaagatac ttaagaatat ccctctccca tcggacgagc ggattttttc
      121 ttcatgggcc tcaggagaag acgcggctct ttggaagata gacgagaaaa gggccgggat
      181 tcttaccgtt gatttcatca ctccggtagt cgacgatcct cttgtttggg gaaggattgc
      241 cgctgcaaat tcgctaagcg atgttttcgc tatgggcggt ttccccttgg tcgcattgaa
      301 catcgtggga tttccggtaa aaagtcttga tatgagcgta ttagaggctg ttttggccgg
      361 agggttcgaa aaggttcggg aggcgggagc cttcctcctt ggcggacaca gcgtcgagga
      421 taaagaaccg aaatacggcc tcgttgcgta cggcgaggtt gctatggatt ctgtatggag
      481 ggtaacgggc gcctgtcccg gagatatgct gctcctgacc aaaccaatcg ggacggggat
      541 tgtagccaca gcgctgaagg cggatatgct ggaggatgaa cgtagcctgc aggaggcaat
      601 tcggtggatg tcgatgttga atgatcttcc gcgacgcctg gataccggcc tccttacatc
      661 tgttcgcgca tgcaccgatg taacagggtt cgggttagcg ggccatgcat tggatatgct
      721 ctcctcgaaa gagatgaacc tggtcctggg catccacgat ataccgttgc ttcccggaac
      781 catcagcctt gcggagtcag gcctcatacc tgccggaaca tacaataacc gtatcgaata
      841 cgaagaccga gttcgcgatc cccagaaaca tgatgaagtg ttaatggata tgctcttcga
      901 tgcccagact tctggcggtc tgttactggc tcttcctgag gaaaaggttc aagattttct
      961 caaagcggtc caaggtatag gatttgaatt gagttctgtt attggacggt ttacgaaggg
     1021 aaacggcgaa cttgaaattg tagacaatat atagtatcat agccaaggaa tattttcttt
     1081 ccatcgtgtt tatcgaggaa agatagggga tgtgtttcct tgttgctatc acaaaaaaag
     1141 tcgcggagag tccggtttgt actgagtctt tcaatgtttt gtgctatgtt aggtatagga
     1201 attattgcac cggtcatacc tctttatgcg aagtctcttg gcgcaagcag cgtcgcgatt
     1261 ggattcataa ttggctcttt ttctttttcc cgtatttggg ggatgcttgt gtcgggagaa
     1321 ctcgctgaac gtatggaccg gaaaaagctt cttttaggcg gtctttttgt gtatgcggcg
     1381 gcttcggttg cctatacgtt ttctttttca accgagatgc tgattttcat aaggacgttg
     1441 cacgggatag gttctgcgat ggtagtgccg attacgatgg cgatcgcggc cgatattgct
     1501 ccaaagggcg aagaaggagc aattttcggg tctcttcagg cggccttgtt tcttggggtt
     1561 ggttccggtc cgcttctcag cggtttctta gccgacactt ttgggttcta tgctccgttt
     1621 tacgccatga cagcactcac tctcctcgcc ctttttctcg ttctggccct cttcccctct
     1681 tccccaccga aaaatacagt caaggaagaa gtgggcgcct tgaaagcttt taagggcatc
     1741 atttctgatc cggagatgct caacgtattt attttccagt tttcttcggc gatgtgccgt
     1801 ggtgttctcg taatggttat tcctcttttt gcttcgcagt ttgacctttc cttgtcgagg
     1861 atcggcatta ttgtttcact gaattctatt tcgaccggta ttctacagaa tgtatccggt
     1921 aaaatggcgg ataatgtgca taaaaatacc ctgatcatta ttggtggagt gatttcagct
     1981 gtgacgctga taggactccc taatttgacg acacctctca gcctctctgt agcaagcgtg
     2041 gctttcggag tcgggcatgc gtttgcttcc ccttcgcttg ctgcgattgc agcggctagg
     2101 ggtaaaaact tcggttcagg gaggacgatg ggccttttta atattgcatt cagcataggc
     2161 atgacgattg gccctataat ggtcggctct ttgttggaca ttaccggaaa cggcatacct
     2221 ttttatatcc taagcgcttt tctggcagct tctgcgcttc cattttttcc ggtttttaaa
     2281 cgcggcgtgt gctaaaaaaa gagacgggag aagtcctccc gttcagaatg gtttttttat
     2341 ttctgcgggg tacggagttg tctcagagcg gcaagtctgt tccacaagaa aatatactga
     2401 tcgtgaaaga aa
//
LOCUS       DAOTIL010000391         2405 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminobacteriaceae bacterium isolate AMR_MDS_3026
            NODE_52207_length_2405_cov_3.891064, whole genome shotgun sequence.
ACCESSION   DAOTIL010000391 DAOTIL010000000
VERSION     DAOTIL010000391.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868050
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminobacteriaceae bacterium (wastewater metagenome)
  ORGANISM  Aminobacteriaceae bacterium
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae.
REFERENCE   1  (bases 1 to 2405)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2405)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:19:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,224
            CDSs (total)                      :: 3,198
            Genes (coding)                    :: 3,184
            CDSs (with protein)               :: 3,184
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2405
                     /organism="Aminobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_52207_length_2405_cov_3.891064"
                     /isolate="AMR_MDS_3026"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:3034609"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..383)
                     /gene="rpsE"
                     /locus_tag="P5201_08525"
     CDS             complement(<1..383)
                     /gene="rpsE"
                     /locus_tag="P5201_08525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013048045.1"
                     /note="located at the back of the 30S subunit body where
                     it stabilizes the conformation of the head with respect to
                     the body; contacts S4 and S8; with S4 and S12 plays a role
                     in translational accuracy; mutations in this gene result
                     in spectinomycin resistance; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S5"
                     /protein_id="HRV98620.1"
                     /translation="MIKKKLDAKGMELTERVVAINRVSKVVKGGKRFKFSVLVVVGDG
                     DRYVGVGMGKAKEISEAVRKGIDKAGKNLLEIKKIGNTIPHPIIGGFGAAEVLLKPAA
                     PGTGVIAGGVVRAIMFRLRSSFRSR"
     gene            complement(400..765)
                     /gene="rplR"
                     /locus_tag="P5201_08530"
     CDS             complement(400..765)
                     /gene="rplR"
                     /locus_tag="P5201_08530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013048044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L18"
                     /protein_id="HRV98621.1"
                     /translation="MKTKTRSILREIRHARLRKTLSGTVEKPRMAVFHSLKHIYVQFI
                     DDERGHTLAAASTLDPALRGTLSGTCNIETAKIIGKIAAERALAKGITTVVFDRGGHM
                     FHGKVKALADAAREAGLKF"
     gene            complement(782..1321)
                     /gene="rplF"
                     /locus_tag="P5201_08535"
     CDS             complement(782..1321)
                     /gene="rplF"
                     /locus_tag="P5201_08535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008372550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L6"
                     /protein_id="HRV98622.1"
                     /translation="MSRIGRKPIPVPSGTTVAVEGGAVVVKGPKGRLETPMVPGIDVA
                     VEGGIIVVTRQNEQKQTKAFHGLIRALVANMITGVSGSFKKDLEIVGVGYRAQMQGKK
                     LILNLGYSNPVEFEAPEGIELALDGPTKLSVKGIDKQAVGQVSAIIRGFRPPEPYQGK
                     GIRYAGEHIIRKAGKTGAK"
     gene            complement(1338..1742)
                     /gene="rpsH"
                     /locus_tag="P5201_08540"
     CDS             complement(1338..1742)
                     /gene="rpsH"
                     /locus_tag="P5201_08540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014806379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S8"
                     /protein_id="HRV98623.1"
                     /translation="MYVTDPIADMLTRVRNANMVYHESVDVPMSKIKMEIARILKEAG
                     YIKNFKVVNDPKKAYSSIRLFLSYGPNRERVIQGLRRISKPGRRVYVGHADLPKVMGG
                     LGIAVISTPKGLKTDTEAGTLGLGGEVLCYVW"
     gene            complement(1771..1956)
                     /locus_tag="P5201_08545"
     CDS             complement(1771..1956)
                     /locus_tag="P5201_08545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003567540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type Z 30S ribosomal protein S14"
                     /protein_id="HRV98624.1"
                     /translation="MARKAIKHKAAQPPKFAVRKYNRCPLCGRVHAFMRRFNMCRCCF
                     RKLAREGKIPGVVKSSW"
     gene            complement(1973..>2405)
                     /gene="rplE"
                     /locus_tag="P5201_08550"
     CDS             complement(1973..>2405)
                     /gene="rplE"
                     /locus_tag="P5201_08550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009201475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="50S ribosomal protein L5"
                     /protein_id="HRV98625.1"
                     /translation="NIGVGDAKLDIKFMDNAIAELGAITGQRPLLKRAKKSVAGFKVR
                     QGMPVACTVTLRGERMWEFLDRLLSLALPSIKDFQGVSRRGFDGRGNYNLGLKEQLIF
                     PEISYDKVIRSRGMNVSLVTSAKTDEEAQALLRELGMPFTR"
BASE COUNT          444 a          731 c          639 g          591 t
ORIGIN      
        1 aaccggctcc ggaaagaact ccggaggcga aacatgattg cacggaccac tccgcccgcg
       61 ataacgcctg ttccgggcgc cgccggtttg aggagtacct cggctgcgcc gaagcctccg
      121 atgatgggat gaggaatggt gtttcctatc ttcttgatct caagaagatt cttccccgcc
      181 ttgtcgattc ctttgcggac ggcctccgaa atttccttgg ccttccccat gccgacgcct
      241 acatagcggt ctccgtcgcc aacgacgacg aggacgctga acttgaacct ttttccgcct
      301 ttgacgactt tgctcactcg gtttattgct actacgcgtt cagtgagctc catccccttg
      361 gcatccaact tctttttaat catcgcatgc gcctcccagt tagaacttca gtccggcttc
      421 acgggctgca tcggccaggg ccttgacttt tccgtggaac atatggccgc ccctgtcgaa
      481 aacaaccgta gtgatgcctt tcgcgagggc gcgctcggcg gctatttttc cgatgatctt
      541 cgccgtctcg atgttgcagg ttccggagag ggttccccgg agtgccggat cgagtgtgga
      601 cgccgccgcg agagtgtgtc ccctctcatc gtcgataaac tgaacataga tgtgcttcaa
      661 gctatgaaac acagccattc tcggtttttc caccgtaccg gagagggttt tccgaagccg
      721 ggcatgccga atctcacgca gtatgcttct ggttttagtt ttcatgtcca ttcacctcga
      781 attacttcgc tccggtcttt ccggccttgc gaatgatatg ctcgccggcg taccggattc
      841 ccttaccctg atacggctcg ggcggacgga aaccgcgaat gatcgcggat acctgcccta
      901 ccgcctgttt gtcgatcccc ttgaccgaaa gcttcgtcgg tccgtccagg gctaactcga
      961 tgccttcggg agcttcgaac tccacgggat ttgagtagcc gaggttgagg atgagcttct
     1021 tcccctgcat ctgtgcacga tagccgacgc cgacgatttc aagatccttc ttgaaactgc
     1081 cgctgacgcc tgtgatcatg ttggcgacaa gcgcacggat cagaccgtgg aacgctttcg
     1141 tctgtttctg ttcgttctgc cgggtgacga cgattatgcc gccttcgacg gcgacgtcga
     1201 tccccggaac catgggcgtt tcgagccgcc ctttcggccc cttcaccacg acggcgccgc
     1261 cttcgacggc gacggtcgtt ccggagggta ccggaattgg cttgcgtccg attcgagaca
     1321 tctactccac ctcttcctta ccacacgtag cagaggactt cgccgccgag gccgagcgtt
     1381 ccggcttccg tgtcggtttt gaggcccttc ggtgtggaga tgacggcgat gccgaggccg
     1441 cccatgacct tgggaaggtc ggcgtgtcca acgtagactc ttctgcccgg tttgctgata
     1501 cggcgcagcc cctggatgac cctctcgcgg ttcggtccgt aggagaggaa caacctgatt
     1561 gagctgtagg ccttcttcgg gtcgttgact accttgaaat tcttaatata cccggcttcc
     1621 ttcaggatgc gggcaatttc catctttatc ttgctcatcg gaacgtcgac gctctcatga
     1681 tacaccatgt ttgcgttcct gacgcgagtg agcatatccg ctatcggatc ggtaacgtac
     1741 attcctagtg acccccttca gtccactcga ctaccagctc gacttgacga cgccgggaat
     1801 tttcccttcg cgagcaagct tcctgaaaca gcagcggcac atgttgaatc ttctcatgaa
     1861 ggcatgaaca cggccgcaga gcggacatct gttatacttt cgcaccgcga acttcggggg
     1921 ttgcgccgct ttatgcttga ttgcctttct cgccattttc gactccccct tcctaccggg
     1981 tgaacggcat tcccagctcg cggagaagag cttgggcttc ctcatccgtc ttcgccgagg
     2041 ttacaagcga gacgttcatg ccacgggaac ggatgacctt gtcgtaactg atctcaggga
     2101 agataagctg ctccttgaga ccgagattgt agttgcctct tccgtcgaaa ccgcggcggg
     2161 agacgccctg aaagtccttg atgctgggaa gcgcgagcga aaggagacga tcaagaaact
     2221 cccacatacg ctctccgcgc agcgtgacgg tgcacgctac gggcattccc tggcggacct
     2281 tgaatccggc gaccgatttc ttcgctctct tcaaaagcgg acgctgtcct gtaatcgcgc
     2341 caagctcagc gatcgcgttg tccatgaatt tgatgtcgag ctttgcgtcg cccacaccga
     2401 tgttc
//
LOCUS       DAOTIL010000392         2404 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminobacteriaceae bacterium isolate AMR_MDS_3026
            NODE_52246_length_2404_cov_3.896977, whole genome shotgun sequence.
ACCESSION   DAOTIL010000392 DAOTIL010000000
VERSION     DAOTIL010000392.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868050
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminobacteriaceae bacterium (wastewater metagenome)
  ORGANISM  Aminobacteriaceae bacterium
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae.
REFERENCE   1  (bases 1 to 2404)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2404)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:19:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,224
            CDSs (total)                      :: 3,198
            Genes (coding)                    :: 3,184
            CDSs (with protein)               :: 3,184
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2404
                     /organism="Aminobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_52246_length_2404_cov_3.896977"
                     /isolate="AMR_MDS_3026"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:3034609"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..284
                     /locus_tag="P5201_08555"
     CDS             <1..284
                     /locus_tag="P5201_08555"
                     /inference="COORDINATES: protein motif:HMM:NF016885.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA];
                     GO_function: GO:0048029 - monosaccharide binding [Evidence
                     IEA];
                     GO_process: GO:0005996 - monosaccharide metabolic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="RbsD/FucU domain-containing protein"
                     /protein_id="HRV98626.1"
                     /translation="RIDLAYALGQPGFFNVLEAILPELAIEGAVWAEEALAKSPMLAG
                     RLETLFQQTPHERIPHEEFKKLLPDVRFVVRTGECTPYANVLLRCGVPF"
     gene            300..1247
                     /locus_tag="P5201_08560"
     CDS             300..1247
                     /locus_tag="P5201_08560"
                     /inference="COORDINATES: protein motif:HMM:NF014684.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HRV98627.1"
                     /translation="MPIFRRFDSRTLLLLLLGGLMLVAGSLEPRFFTGANLLNVLRQS
                     SIIGVISLGMTVIILTGGIDLSVGSVLSLSVLFAASASKTGLPAPVAWACALLAGAGL
                     GWVNGFGVSRLRLPPFIVTLGMMGFARGLSFIYTGGAPITGFDALFRLPGTGVFAGIP
                     APALLFGAAFIIVFVLLERCPWGEHIRNTGSNPVAAWGSGIDVPGTISKAYVLCGLLS
                     ALAGLMLVGRLDSAQPGAGVGYEFGAIAAVVLGGTSFSGGKGTLAGTILGVLIMGVLE
                     NGMNLLNVNPFSEQVVKGVVIAAALVAYGSLGRKSRTAG"
     gene            1415..2293
                     /gene="rbsB"
                     /locus_tag="P5201_08565"
     CDS             1415..2293
                     /gene="rbsB"
                     /locus_tag="P5201_08565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019259653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose ABC transporter substrate-binding protein
                     RbsB"
                     /protein_id="HRV98628.1"
                     /translation="MKKLFAVLLVVLLVAAPAAAAEKTLGLVISTLNNPFFVTLKQGA
                     EEAATKAGVKLVVLDAQDDSAKMTAAMEDLIQQKVDAILVNPTDSDAVTPSIMKANAA
                     GIPVLTIDRGASGGQVLCHVASDNVAGGVMAGEFLAKKLEAKGNVVELVGIPGTSAAR
                     DRGKGFNDAIATFKDIKVVASQAADFSRDKGLKVFENILQAQPEIAGVFAHNDEMILG
                     AVAAAEAAGRTGIVFVGFDAVDDAVAAVKAGKLAATVAQQPAVIGAMGVEKAVAHLGG
                     EKLPEFIPVPLQLVVE"
BASE COUNT          411 a          818 c          762 g          413 t
ORIGIN      
        1 cacggatcga cctggcctac gcgctcgggc aacccggctt cttcaacgtg ctggaagcca
       61 tccttcccga actcgcaatc gaaggcgccg tctgggccga agaggcgctc gccaaaagtc
      121 cgatgctcgc cggacggctg gaaacgctct tccagcagac cccgcacgaa cgcattccgc
      181 acgaagagtt caagaagctc ctgcccgacg tccgcttcgt cgtccgcacg ggcgagtgca
      241 caccgtacgc caacgtgctg ctccgctgcg gcgttccgtt ctaaagaagg cggaaggccg
      301 tgccgatctt tcggcgtttc gactcgcgaa cgctgctcct gctgcttctg ggggggctca
      361 tgcttgttgc gggctcgctg gagccgcgct ttttcaccgg cgccaacctg ctgaacgtgc
      421 tgcgccagtc ctccatcatc ggcgtcatct cgctgggaat gaccgtgatc atcctcaccg
      481 gaggcatcga cctctccgtc ggcagcgtcc tctcgctctc cgtcctcttc gccgcctccg
      541 cgtcgaaaac cggcctcccc gcccccgtcg cgtgggcgtg cgccctcctc gcgggagccg
      601 ggctgggctg ggtcaacggc ttcggcgtct cacggctgcg cctccccccc ttcatcgtca
      661 cgctgggaat gatggggttc gcgcgcggac tctccttcat ctacacgggc ggcgcgccca
      721 tcaccgggtt cgacgctctc ttcaggctgc cgggaacggg cgtcttcgct ggaattcccg
      781 cgcccgcgct gctcttcgga gcggccttca tcatcgtatt cgtcctcctc gaacgctgcc
      841 cgtgggggga gcatatccgc aacaccggct cgaaccccgt cgccgcctgg ggatcgggga
      901 tcgacgtccc cggaacgatc tcgaaggcgt acgtcctctg cggcctcctc tccgcgctcg
      961 cgggactaat gctcgtagga aggctcgact ccgcgcagcc cggcgcgggc gtcggctacg
     1021 aattcggcgc catcgccgcc gtcgtgctcg gcggaaccag cttctcggga ggcaagggaa
     1081 cgctcgccgg aaccatcctc ggcgtgctga tcatgggcgt gctggagaac gggatgaacc
     1141 tgctcaacgt caatcccttc tccgaacagg tggtgaaggg ggtggtcatc gcggcggccc
     1201 tcgtggccta cggaagcctg gggcggaaga gccggacggc ggggtagcgc ctgcttgccg
     1261 tattccggac agccgcacgg ctcaaggaag tccgcgaaga gcgcatgcac atcttgaacg
     1321 attcatacgc gcttcccggc gtaccaaacc tgtttccaca acgatgcgga ggcgtaaaac
     1381 gccccgcgcg aaaattcaag gaggtgtccg tttcatgaaa aagttgtttg ccgtactgct
     1441 ggtggttctg ctcgtcgccg ctcccgcggc ggcagcggag aagacgctcg gcctggtgat
     1501 ctccacgctg aacaacccct tcttcgtcac gctgaagcag ggggccgaag aggccgcaac
     1561 aaaggcgggc gtgaagctcg tggtactgga cgctcaggac gactcggcga agatgaccgc
     1621 cgcgatggaa gacctgattc agcagaaggt cgacgccatt ctggtgaacc cgaccgactc
     1681 cgacgccgtc acgccctcca tcatgaaggc caacgccgcg ggcatccccg tgctcaccat
     1741 cgacaggggc gcgtccggcg gacaggttct ctgccacgtg gccagcgaca acgtcgcggg
     1801 cggcgtcatg gccggagagt tcctcgccaa gaagctcgag gccaagggga acgtcgtcga
     1861 gctggtcggc atccccggaa cctccgccgc cagagaccgc ggcaagggat tcaacgacgc
     1921 tattgccaca ttcaaggata tcaaggtcgt cgcctcgcag gccgccgact tcagccgcga
     1981 caaggggctc aaggtcttcg agaacatcct ccaggcgcag ccggagatcg cgggcgtctt
     2041 cgcccacaac gacgagatga tcctcggcgc ggtcgccgcg gcagaagccg ccggacgcac
     2101 cggcatcgtc ttcgtcggct tcgacgcggt ggacgacgcc gtggctgcgg tcaaggcggg
     2161 caaactcgcc gcgacggtcg cccagcagcc tgcggtcatc ggcgcgatgg gcgtcgagaa
     2221 ggcggtcgcg cacctcggcg gcgagaagct gcccgagttc atccccgtgc cgctgcagct
     2281 cgtcgtcgaa taggcttgac aacagatgaa gtaccgggac gccgtcgttc gaggacggcg
     2341 tccctcccat cggaggttga tggcgtgaca ctcttgctgg agatgaagaa cgtacacaag
     2401 cgct
//
LOCUS       DAOTIL010000393         2402 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminobacteriaceae bacterium isolate AMR_MDS_3026
            NODE_52313_length_2402_cov_3.660844, whole genome shotgun sequence.
ACCESSION   DAOTIL010000393 DAOTIL010000000
VERSION     DAOTIL010000393.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868050
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminobacteriaceae bacterium (wastewater metagenome)
  ORGANISM  Aminobacteriaceae bacterium
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae.
REFERENCE   1  (bases 1 to 2402)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2402)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:19:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,224
            CDSs (total)                      :: 3,198
            Genes (coding)                    :: 3,184
            CDSs (with protein)               :: 3,184
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2402
                     /organism="Aminobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_52313_length_2402_cov_3.660844"
                     /isolate="AMR_MDS_3026"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:3034609"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..631)
                     /locus_tag="P5201_08570"
     CDS             complement(<1..631)
                     /locus_tag="P5201_08570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013049248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016625 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, iron-sulfur
                     protein as acceptor [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde ferredoxin oxidoreductase N-terminal
                     domain-containing protein"
                     /protein_id="HRV98629.1"
                     /translation="MDKFGSIVRINLTTGSIKKESLCEKTMKEFIGGRGYATKVLYDE
                     LPKGTDPLSPENKLIFANGPLTGKSAPAAGRYMVVTKSPLNNYIASSNSGGFWGAELA
                     RAGWFMIILEGKAQKPSYIWINDDKVEIRDASAFWGLDSHETTDKLLAAVGDDKAKVA
                     CIGPAGERLSKLASVMNDKNRAAGRSGVGAVMGSKNLKAIVVKGSSRPHV"
     gene            936..1766
                     /locus_tag="P5201_08575"
     CDS             936..1766
                     /locus_tag="P5201_08575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014806671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008641 - ubiquitin-like modifier
                     activating enzyme activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HesA/MoeB/ThiF family protein"
                     /protein_id="HRV98630.1"
                     /translation="MLSWFDFLPLRADRQGPWQVVSFLEVREAAEDEGLSPVEAEIRC
                     LQRGYVPSRYIRSMGTIGIDGQIRLLSSAVAVIGCGGLGGLVADLLARAGVGRLVLVD
                     GDVFDETNLNRQILSFEGNLGLSKSKVAAQRVTEINGVVRAEGRQCMFDEFTADGILE
                     GCDLAVDCLDNLSTRRILFSECRTRGIPVVSGAIGGFWGQIGVAMPDEQNLAEFLSGD
                     TDKGVETETGNPPFTPAVIAALECAQAVKILTGKGKLLTDQLLWMDLAEDEFTRLRLS
                     "
     gene            1788..>2402
                     /locus_tag="P5201_08580"
     CDS             1788..>2402
                     /locus_tag="P5201_08580"
                     /inference="COORDINATES: protein motif:HMM:TIGR00654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PhzF family phenazine biosynthesis protein"
                     /protein_id="HRV98631.1"
                     /translation="MKLFQVDAFTDRLFAGNPAGVCLLEAEVPDAWMQSMAAEMNLSE
                     TAFLLGTPSGWSLRWFTPRVEVALCGHATLSTAHIMLQEGMLQQGHEAVFHTKSGPLS
                     ARAEDEWIVLDFPAKKVVPVAPPPGLIEALGLERDEYLFCGKNKDDYLLELPDEELVR
                     SLDPDFVALSDVEARGTMVTAPSSRCEFDFASRFFAPAVGVNEDP"
BASE COUNT          435 a          684 c          733 g          550 t
ORIGIN      
        1 cgacgtgggg gcggctgctt cccttgacga cgatcgcctt caggttcttc gagcccatga
       61 cggcgccgac gccgcttcgt cccgcagccc tgttcttgtc gttcatcacg gaggcgagtt
      121 tggaaaggcg ctctccggcg gggcctatgc aggcaacctt cgccttgtcg tcgccgacgg
      181 ccgcgaggag cttgtcggtt gtttcatggc tgtcgagccc ccagaatgcg gaggcgtcgc
      241 ggatttcaac tttgtcgtcg ttgatccaga tataggatgg tttctgggcc ttgccttcaa
      301 gaatgatcat gaaccatcct gcgcgagcca gctcggcgcc ccagaatccg ccggagttgc
      361 tgcttgcgat atagttgttg agagggctct tcgtgaccac catgtagcgc ccggctgccg
      421 gagcggactt ccctgtaagc ggaccgttgg cgaagatcag cttgttctcc ggggaaagcg
      481 ggtcggtacc tttggggagc tcgtcgtaga gcacctttgt cgcgtaccct cgtcctccga
      541 taaactcctt catcgttttc tcgcacagac tttccttttt gatgctgccg gtggtgaggt
      601 tgatacgcac aatcgatccg aacttatcca taagcagttc cctccttgtg tattcgttca
      661 tcttgcaata cccttgttgc cgatacaaaa aaaagaagaa tgacagcctg taaagctgtt
      721 catccttctt tgcaaacacg gccggcggga agatcactct tccaacccga tgcccggcac
      781 atctcgggga gacgcggcgg gtcgaaggca tattcggttg gcttccttgg aacatttatt
      841 agttcacttt atttttatac cataaatggc gacctgtcaa ttctccgcag ggtattttcc
      901 ttgtataatc attctgcgca tcgggaggtg agtgtgtgtt gagttggttt gattttcttc
      961 ctttaagagc ggaccggcag gggccgtggc aggttgtttc gttcctggag gtgcgcgaag
     1021 ccgcggagga tgaaggcctg tcgccggtcg aagccgaaat tcgctgtctg cagcgcgggt
     1081 acgttccttc cagatacatc agaagtatgg gcactatcgg catcgacggc cagatcaggc
     1141 tgctgtcctc cgccgtggcg gtgataggct gcgggggact tggcggcctt gtcgccgatc
     1201 ttcttgcgcg ggccggagtg ggacgtctgg tgctggtcga cggcgacgtc ttcgacgaga
     1261 cgaatctgaa ccgtcaaata ctctccttcg aggggaacct tggactctcc aaatcaaagg
     1321 ttgcggcgca gcgcgttacg gagatcaacg gagtcgtccg cgccgagggt cggcagtgca
     1381 tgttcgacga attcaccgcg gacggcatac tcgaagggtg cgatctggcc gtggactgcc
     1441 tggataacct gagcacgcgg cgcattctct tctccgagtg ccggaccagg ggaatcccgg
     1501 tagtctcggg ggccatcggc ggcttctggg ggcaaatcgg cgtcgccatg cctgacgaac
     1561 agaaccttgc ggaattcctg tcgggcgata cggacaaggg ggtcgagacc gaaaccggca
     1621 accctccctt tactcctgct gtgatcgccg cgcttgaatg cgctcaggcc gtgaagatcc
     1681 tcactggcaa gggaaaactc ctcacggacc agctgttgtg gatggatctc gccgaggacg
     1741 agttcacgcg gcttcgcctg agctgaaaaa cgaaaggggg aaacggcatg aaactttttc
     1801 aagtcgatgc gttcaccgat cgtctcttcg caggaaatcc ggcgggcgtc tgtctgctcg
     1861 aagctgaggt tcctgatgcc tggatgcaga gcatggccgc ggagatgaac ctctccgaga
     1921 cggcgttcct tctgggaacc ccatccgggt ggtccctccg ctggtttacg ccgcgcgtcg
     1981 aggtggcttt gtgcggccac gcgacgctct ccacggcgca tattatgctc caggagggaa
     2041 tgcttcagca ggggcatgag gctgtctttc acacgaagag cggccctttg agcgcacgag
     2101 ccgaggatga atggatcgtg ctcgactttc cggcgaagaa ggtcgttccc gtcgcccctc
     2161 ctcccgggct catcgaggcg ctcgggctgg agcgcgacga atacctcttc tgcgggaaga
     2221 acaaggacga ctacctcctt gagctccccg acgaggagct tgtacgctcg ctcgatcccg
     2281 acttcgtcgc gctctccgat gtggaggcca ggggaaccat ggtgacggcg ccgtcgtcgc
     2341 gctgcgagtt cgactttgca tcccgcttct tcgcgcctgc tgttggcgtg aacgaagacc
     2401 cc
//
LOCUS       DAOTIL010000394         2400 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminobacteriaceae bacterium isolate AMR_MDS_3026
            NODE_52374_length_2400_cov_3.521109, whole genome shotgun sequence.
ACCESSION   DAOTIL010000394 DAOTIL010000000
VERSION     DAOTIL010000394.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868050
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminobacteriaceae bacterium (wastewater metagenome)
  ORGANISM  Aminobacteriaceae bacterium
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae.
REFERENCE   1  (bases 1 to 2400)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2400)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:19:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,224
            CDSs (total)                      :: 3,198
            Genes (coding)                    :: 3,184
            CDSs (with protein)               :: 3,184
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2400
                     /organism="Aminobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_52374_length_2400_cov_3.521109"
                     /isolate="AMR_MDS_3026"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:3034609"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..235)
                     /locus_tag="P5201_08585"
     CDS             complement(<1..235)
                     /locus_tag="P5201_08585"
                     /inference="COORDINATES: protein motif:HMM:NF024803.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HRV98632.1"
                     /translation="MKTTEAYPSLLLIGQFSKLSGLSCRMLRFYAEQNILVPAYTDCS
                     TGYRHYLASQLTEAKLLKQLRQADFSLGDIAEIL"
     gene            complement(232..501)
                     /locus_tag="P5201_08590"
     CDS             complement(232..501)
                     /locus_tag="P5201_08590"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRV98633.1"
                     /translation="MDEFGRSLRSFGLNPDQVRDALRCGDIREVLDSLKKEEETLLLR
                     RVEIADLRERLLAMAGEERLRIHDEISDDDLELMSAAGQEPRNIP"
     gene            787..1065
                     /locus_tag="P5201_08595"
     CDS             787..1065
                     /locus_tag="P5201_08595"
                     /inference="COORDINATES: protein motif:HMM:TIGR03798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Nif11-like leader peptide family natural product
                     precursor"
                     /protein_id="HRV98634.1"
                     /translation="MSVEAAAAFYDRLENDLALMGKLKELGDKERLVLYVKDELGYDF
                     SREEMRKVLFERNPELSEEELQSVVGGAGGNGGSDGLSVANVIVTAGL"
     gene            1147..>2400
                     /locus_tag="P5201_08600"
     CDS             1147..>2400
                     /locus_tag="P5201_08600"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRV98635.1"
                     /translation="MRKYRGMPMVLVVLLMTAVMFGGGCGGGSDRMPAHPGEIQPGAE
                     YEGVLSVQDGDEDRVLMRTRYEAHNIETGDSMEPVVALFVKEEEHLDEATGTMRVLSA
                     SEISRPTPEQVRAFLKDFWDAEHGGPADEGVNAREFLDFLAAHNMSLDQFFRYFEALD
                     SSVSAFIDVIETYSTKLSSFDGKDIYGETESFCDLLGLELREYFQKIILVFGTHAAYC
                     DKLVMLGGIGKSTLYDAYSKWLSDQEVAPEDPFVAFLNYLKARSAEASAARPSGAAET
                     ALEVAKFAWDIIKDGKAQVTTDGAFTSVLRKDTTALDYGYAKRNETSIIHFKITDAWI
                     KSWVLIETKFRGSASYDAVNPTFGGKYLQNVQFDVESAYAQWGMYLNVSAQVSNVTNP
                     ASVENPDPEIDVVARINAGWLFQSFG"
BASE COUNT          508 a          615 c          729 g          548 t
ORIGIN      
        1 tcagaatctc cgcaatgtcg ccgagcgaga aatcggcttg gcgaagctgc ttcaggagct
       61 tcgcttcggt gagctgagaa gcgagatagt gccggtagcc cgtcgagcag tccgtgtatg
      121 ccgggaccag aatgttctgc tcggcgtaga agcggagcat acggcaggaa agtccgctca
      181 attttgaaaa ttgcccgatg agcaggagag acgggtaagc ttccgtcgtc ttcatgggat
      241 gtttctcggc tcctggcccg ccgcgctcat cagttcaaga tcgtcgtcgg aaatctcgtc
      301 gtgtattctc agtctctctt ctccggccat ggcgagcagc cgctcgcgga ggtcggcaat
      361 ctcgacgcgt ctcagcagga gcgtctcttc ctcttttttc aaggaatcca gaacttcgcg
      421 gatatcgccg caacgcaggg cgtcgcgaac ttgatccgga ttcaatccga acgaacgcag
      481 cgaccgaccg aactcatcca tcattttccc ccctttgcgg tacccgattt tcggcgttat
      541 cgtacacctt cacatagtgt gagagtcaag actccgagta ttcttcggag agccgccgtt
      601 gatacagggc gccgtcaaag ttcacaaagt atggcacgaa ggcgcgcctc cttcaagtag
      661 gggcggcact attttttatt ttcgcacttg acattgacac agtgtcaatg tttatactgt
      721 cggcagtaag acggggactc ctgtttttcc tgaacgctat tctacgtacg atggggggtg
      781 gtctgtatgt cggttgaagc tgcggcggcg ttttacgacc gtctcgaaaa cgacttggcg
      841 cttatgggaa aattgaagga gttaggcgac aaggagcgat tggtgctcta cgtgaaggac
      901 gaactcgggt acgacttctc acgggaagag atgcggaagg tgctcttcga gcggaatccg
      961 gaactgagcg aagaggaact gcaatcagtc gttggaggcg cggggggcaa cgggggatct
     1021 gacgggcttt ctgttgcaaa tgtgatcgtt acggcaggtc tgtaactcgg attcctcttc
     1081 gttttcccga agaagagagc atgtgtgtga atcgctggtt attttgattg aaaggagtgg
     1141 ttgctcatga ggaaataccg cggaatgccc atggtgttgg tggtgctctt gatgacggcg
     1201 gtgatgttcg gagggggatg cggcggcggg tcggacagaa tgccggcgca tcctggggag
     1261 attcagccgg gagcggaata cgagggtgtg ctttcggttc aggacggcga cgaggaccgg
     1321 gtgctcatgc gcacccgata tgaagcgcat aatatcgaga ccggcgattc gatggagccg
     1381 gtggtcgcgc tctttgtaaa agaggaagag caccttgacg aggcgacggg tacaatgcgc
     1441 gttctgtccg cgtccgaaat ctcaaggccg actcccgaac aagtgagggc gtttctgaaa
     1501 gacttctggg acgcggagca cggcggccct gcggacgaag gcgtcaacgc gcgtgaattt
     1561 ctggattttc ttgccgcgca caatatgtcc cttgatcagt tcttccgcta ttttgaggcg
     1621 ctggactctt ccgtttcggc ttttatcgat gttatcgaga cgtactcaac gaagctgtcc
     1681 tctttcgacg gcaaagacat ttacggggag accgagtctt tctgcgatct cctcggtctg
     1741 gagctgcggg aatattttca aaaaattatc ctggttttcg gaacgcatgc ggcctactgc
     1801 gacaagctgg tcatgctcgg ggggataggg aagtccactc tgtacgacgc gtattcaaaa
     1861 tggttatcgg atcaggaggt cgcaccggaa gacccgtttg tcgcgttctt gaactatctg
     1921 aaggcgcgtt cggcggaagc ctccgccgct cgtccctccg gcgcggccga aaccgccctg
     1981 gaagttgcta agttcgcgtg ggatatcatc aaggacggaa aggcacaggt cactaccgac
     2041 ggcgccttta cgtccgtgct gaggaaagac acgaccgctc ttgattacgg gtacgcgaaa
     2101 cgcaacgaga cgtcgatcat ccactttaag ataaccgacg catggatcaa gtcgtgggtg
     2161 ctcatcgaga ccaaattccg cggttccgct tcgtacgacg cggtcaatcc tactttcggc
     2221 gggaagtacc tgcagaacgt ccagttcgac gtggagagcg cgtacgccca gtggggaatg
     2281 tacctcaacg tgagcgcgca ggtctccaac gtgacgaacc ccgcatcggt ggagaatccc
     2341 gaccccgaga tcgacgtcgt cgcgcgcatc aacgccggtt ggctcttcca gtcgttcggc
//
LOCUS       DAOTIL010000395         2394 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminobacteriaceae bacterium isolate AMR_MDS_3026
            NODE_52527_length_2394_cov_4.570329, whole genome shotgun sequence.
ACCESSION   DAOTIL010000395 DAOTIL010000000
VERSION     DAOTIL010000395.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868050
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminobacteriaceae bacterium (wastewater metagenome)
  ORGANISM  Aminobacteriaceae bacterium
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae.
REFERENCE   1  (bases 1 to 2394)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2394)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:19:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,224
            CDSs (total)                      :: 3,198
            Genes (coding)                    :: 3,184
            CDSs (with protein)               :: 3,184
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2394
                     /organism="Aminobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_52527_length_2394_cov_4.570329"
                     /isolate="AMR_MDS_3026"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:3034609"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..226
                     /locus_tag="P5201_08605"
     CDS             <1..226
                     /locus_tag="P5201_08605"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRV98636.1"
                     /translation="LYLVEWADTAGRKLSVLYSESGGSVESVSAERKRALFENGDLEA
                     CSFPASEVLFPDEVQKLAERFQIVVEEEEE"
     gene            223..1827
                     /locus_tag="P5201_08610"
     CDS             223..1827
                     /locus_tag="P5201_08610"
                     /EC_number="3.5.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF019581.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HRV98637.1"
                     /translation="MSKSVFYNGKIAVPGGFVEGMAIEGQNIVCLGSSEEVCAIRDAE
                     KIDLAGRLALPGFIDGHMHFLAYALSLERADLLGCLSVNEVRSRLRAFLDERRPEPGE
                     WVVGRGWNHELFDDNRILTKEDLDDLTPDNPAMLARVCGHIVVLNSRAITLLGLTGES
                     RFAGGVVDLDDGGEPTGVIRESVVEWAGSKLPLPGPEKLRKLVVRAGEETAKAGLTSI
                     HSDDLGSVGGDFQTIIDLYISLCKEDEMPVRITEQLLLRNRSALGHFLSTGWRTVDGM
                     PFFHIGPLKILTDGSMGGRTALLRKEYEDAPGVFGVPIYGHDELDDLVLTAHLAGMQV
                     ATHAIGDGALNMCLDAMERALLAKPRDARHYIVHCQMGDMEQYRRMARLGIGAAIQPP
                     FVPSDRPMALKRIGEERALAGYAWKTMKELGIFLSGGSDSPVEKFDPLWGVYTAVARK
                     DENGLPQGGWNPSQKFSVAEAVDLYTRGGAYASFEEHRKGTLAEGMLADVVVLDRDIF
                     SVPEDEIKEAHVALTMVGGRIVYRTD"
     gene            complement(1824..2117)
                     /locus_tag="P5201_08615"
     CDS             complement(1824..2117)
                     /locus_tag="P5201_08615"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRV98638.1"
                     /translation="MIYTVRFFGVLAMVLGVQSGAPISLELPEGSEYGSALDAIHEKY
                     GDRFPPSMWDRGKKMFSSGVLAFGDDGRFLPRTRSASLPKCGEIRFHLPLSGG"
BASE COUNT          514 a          613 c          729 g          538 t
ORIGIN      
        1 cctatatctt gtcgagtggg ccgacacggc agggcgaaag ctctccgttc tctattccga
       61 atcgggagga agcgttgaaa gcgtctccgc cgaacggaaa agagcgctct tcgaaaacgg
      121 cgatcttgaa gcctgctctt tccctgcgtc ggaggtgctc ttcccggatg aagttcaaaa
      181 gcttgccgag cgctttcaaa ttgttgtaga ggaggaggaa gagtgagcaa aagcgtgttc
      241 tacaacggga agattgccgt tccgggagga ttcgtcgagg gaatggcaat agaaggacag
      301 aatatcgttt gtctcggaag ttcggaagaa gtgtgcgcga tacgggatgc cgaaaagatt
      361 gatcttgccg gaaggctcgc tctccccggt ttcatagacg ggcacatgca tttcctcgca
      421 tatgcgcttt ctctcgaacg agccgacttg cttggatgtc tttctgtgaa tgaagttcgt
      481 tcgcgtctgc gagcctttct cgacgagaga aggccggaac ccggcgaatg ggttgtcggg
      541 cgaggctgga accatgaact tttcgatgac aaccggattc ttacaaagga agatcttgac
      601 gacctgacgc ccgataaccc cgctatgctt gccagggttt gcggtcatat cgttgtgctc
      661 aactccaggg cgataacgct tcttgggctt acgggcgaga gcaggtttgc cggaggagtt
      721 gttgacctcg acgacggagg cgaacctaca ggcgtcatcc gcgaatcggt cgtggagtgg
      781 gcgggctcaa aactccctct tccggggcct gaaaagctgc ggaaacttgt agtgcgtgca
      841 ggagaagaga ccgctaaagc cgggcttacc tcgattcatt cggacgatct tggcagcgtc
      901 ggcggtgatt ttcagacgat tatcgatctc tacatctctc tctgtaaaga ggacgaaatg
      961 ccggtgagga ttaccgagca actgctgctc cggaaccgct cagcgctggg tcatttcctt
     1021 tcgacaggtt ggcggacagt tgacggcatg ccttttttcc atatcgggcc gctgaagatt
     1081 cttaccgacg gctcaatggg cgggcgaacc gcgcttttgc gaaaagagta tgaagacgct
     1141 ccgggagttt tcggcgtccc tatctacggg cacgacgaac tggacgatct cgttctgacg
     1201 gcgcatctgg cgggtatgca ggtggcgacg cacgctatcg gcgacggcgc gctcaatatg
     1261 tgccttgacg ccatggaacg ggcgctcctg gcaaaacccc gcgacgcgcg tcattatatt
     1321 gtgcattgcc agatggggga tatggagcag taccggcgca tggcgcggct tggaatcggc
     1381 gccgctatcc agcccccgtt cgttccctcg gatcgcccga tggcgttgaa gcgtatcggc
     1441 gaggagcggg cgctcgcagg ctatgcgtgg aagacgatga aggagcttgg aatctttctc
     1501 tcgggcggtt cggattctcc cgtcgagaag ttcgatccgc tgtggggcgt ctataccgcg
     1561 gtcgctcgaa aggacgagaa cggcctgcct caaggcggat ggaatccgtc gcaaaagttc
     1621 tccgtcgccg aggcggtcga tctgtatacg cgaggcggcg catacgcctc attcgaggag
     1681 catcgcaagg gaacgctggc cgaagggatg cttgcggatg tcgttgttct cgacagagac
     1741 atcttctccg ttccggagga tgagataaaa gaagcgcatg ttgccctcac gatggtcgga
     1801 gggcgtattg tctaccggac tgactaacct ccggacaggg ggaggtgaaa acgaatctct
     1861 ccgcacttcg gcagagaggc ggagcgggtg cggggcaaaa aacgcccatc gtcgccaaaa
     1921 gcgagcactc ccgaagaaaa catctttttc cccctgtccc acattgacgg gggaaaacgg
     1981 tcgccgtact tttcatggat ggcgtcgaga gcggagccgt actcgctccc ttcaggcagt
     2041 tccagtgaga ttggcgctcc ggactgaacg ccgagaacca ttgcgaggac gccgaagaag
     2101 cgtacagtat aaatcatcga acccctcctt tctgacaacg atattgtacc gtactccttt
     2161 cggggttgca caggaagaat agttgtataa tacttttcga acatataaat tttaaccgag
     2221 gagaatcgac gatgaattcg aacacatgcc tgcgcgttca ttcttttttc ccacgccgtt
     2281 tactgcggat ggggctgctt ttgttggcag gggttttact tctgcctctg ttagtaacgc
     2341 ccctttttgc agagcgggat accatggagt acgatgtcgt tgtggcggga ggcg
//
LOCUS       DAOTIL010000396         2393 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminobacteriaceae bacterium isolate AMR_MDS_3026
            NODE_52562_length_2393_cov_3.221985, whole genome shotgun sequence.
ACCESSION   DAOTIL010000396 DAOTIL010000000
VERSION     DAOTIL010000396.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868050
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminobacteriaceae bacterium (wastewater metagenome)
  ORGANISM  Aminobacteriaceae bacterium
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae.
REFERENCE   1  (bases 1 to 2393)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2393)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:19:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,224
            CDSs (total)                      :: 3,198
            Genes (coding)                    :: 3,184
            CDSs (with protein)               :: 3,184
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2393
                     /organism="Aminobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_52562_length_2393_cov_3.221985"
                     /isolate="AMR_MDS_3026"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:3034609"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            106..507
                     /locus_tag="P5201_08620"
     CDS             106..507
                     /locus_tag="P5201_08620"
                     /inference="COORDINATES: protein motif:HMM:NF013098.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HRV98639.1"
                     /translation="MPEICSQITFFYYKNFERARDFYERIMGFELADDQGTCRIYRVQ
                     GTSFLGIVDEKHGHCSAPRSESNVLVTLATNDVKGWWEHLRKEGVKITSELLWKPEIS
                     IEAFFFEDPEGYALEVQSFLKPELRKIFGQE"
     gene            complement(576..1376)
                     /locus_tag="P5201_08625"
     CDS             complement(576..1376)
                     /locus_tag="P5201_08625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013049236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_process: GO:0030001 - metal ion transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter permease"
                     /protein_id="HRV98640.1"
                     /translation="MIELLQFEFMRNALWAALLCSGLCGILGTVVVVKRYVILAGGVA
                     HAAYGGVGIALYLGISPQLGAVGFSLALSLLMAHVMLKREARADSIIGVLWAAGMAAG
                     VIFADLTPGYGADLMSYLFGSILTVAKADLFMMGGLLAVTLVLGFVWRRQIAAFSFDE
                     DFARTRGVPVALLHFGAVVLLSMAVVLIIRIVGLILVIALLTIPPSVAEKFSRSLWSM
                     MGISCVLSALFSVAGLALAVQFNLTAGAVIIAVASAVYLVSLLLPGRR"
     gene            complement(1369..2160)
                     /locus_tag="P5201_08630"
     CDS             complement(1369..2160)
                     /locus_tag="P5201_08630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013049237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HRV98641.1"
                     /translation="MHTESALSFTNVAFSYGGDPVLQDVSFSVPAGEFLVIMGPNGGG
                     KTTLLKLALGLLSPGSGTVLIHGRAPGEDRSAAGYVPQDAGRNRDFPITALEAVLHGR
                     LGAGSHGLFYSVADKTAARDALERMGVESLASRPMGELSQGQRQRVLIARALASEPKV
                     LLLDEPLASIDPETRDMLLTELASLRSRMTVIMVSHDISAIPGRATAVACVNRNVFYH
                     DSGEILPGMFEKVWGTCPVELVAHGIPHRVLAVHTHDHSEDCAHD"
     gene            complement(2166..>2393)
                     /locus_tag="P5201_08635"
     CDS             complement(2166..>2393)
                     /locus_tag="P5201_08635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005659148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRV98642.1"
                     /translation="WRNREKWEDYQKAAGEMLVRTGTPYAPWTVVATNSKKMARIKIL
                     TTVTEGIRKALDGRAARQKPGEPRNTKRKKA"
BASE COUNT          470 a          777 c          758 g          388 t
ORIGIN      
        1 gcaggagctt gcgcggctcg tgcgcatctg taaaacgttc tcagcggagc gccttgctgc
       61 acgataacgg cgcagcaagg cagaaatcaa tgggaggaat gcttcatgcc tgaaatctgt
      121 tcacagatca ctttttttta ctacaagaac ttcgagaggg cgcgcgactt ttacgagcgg
      181 atcatggggt tcgaactcgc ggacgaccag gggacgtgcc gtatctaccg ggtgcagggg
      241 acgagcttcc tcggaatcgt cgacgagaag cacgggcact gctccgcgcc ccgctcggag
      301 agcaacgtcc tcgtcacgct ggcgacgaac gacgtcaagg ggtggtggga gcacttgcgg
      361 aaggaaggcg tcaagataac ctccgagctg ctttggaaac cggagatttc tatcgaggcg
      421 ttcttcttcg aggatccgga ggggtacgcc ctcgaagtcc agtctttcct gaaaccggag
      481 ctgcggaaga ttttcgggca ggagtgatac ggcgctacgc ggcgaaacgt gaagcggtag
      541 ctcccctccg aagtacaaag agcggaggag gggagttacc gtctccccgg cagtagcagc
      601 gacaccagat agaccgccga ggccaccgcg atgatcaccg cgcccgcggt gaggttgaat
      661 tgtaccgcga gggcgagccc ggcgacggag aagagcgcgc tcaaaacgca cgaaatgccc
      721 atcatcgacc aaagcgagcg ggagaacttc tccgccacgg atggggggat ggtgagcagc
      781 gcgatgacca gaatcagccc gacgatgcgt atgatgagca ccacggccat cgagagcagc
      841 accaccgcgc cgaagtggag gagcgcgacg ggaacgcccc gcgtacgggc gaagtcctcg
      901 tcgaacgaga aggcggcgat ctgcctgcgc cagacgaatc cgagaacgag cgtcacagcg
      961 agcagtccgc ccatcatgaa gaggtcggcc ttggcgacgg tgaggatgct gccgaagaga
     1021 tagctcatca gatcggctcc gtaccccggc gtaaggtcgg cgaagatgac gcccgccgcc
     1081 attcctgcgg cccagagcac accgatgatg gagtccgccc gcgcctcccg cttcagcatc
     1141 acgtgcgcca tcagcagcga aagagccagc gagaagccca cggccccgag ctgcggtgaa
     1201 atcccgaggt agagcgcgat gcccacgccg ccgtacgccg catgcgcgac gccccccgcg
     1261 aggatgacgt agcgcttcac cacgaccacg gtcccgagga tgccgcagag cccgctgcac
     1321 agcagggccg cccagagggc gttccgcatg aactcgaact ggagcagctc aatcatgagc
     1381 gcagtcctcg gaatggtcgt gcgtgtggac ggcgagcacc ctgtgcggga tgccgtgcgc
     1441 caccagctcc acgggacacg tcccccagac cttctcgaac atgcccggga gaatctcccc
     1501 cgagtcgtgg tagaagacgt tccggttgac gcacgccacg gctgtggcgc gccccggaat
     1561 ggcggagatg tcgtggctca ccatgatcac cgtcatcctc gaacggagcg aggccagctc
     1621 cgtcagcagc atgtcgcgcg tctccgggtc gatgctcgcc aacggttcgt cgagcagcag
     1681 caccttcggc tccgaagcca gcgcgcgcgc gatcagcacc cgctgccgct gcccctgcga
     1741 aagttcgccc atcggccggg aggccagaga ctccaccccc attcgttcga gcgcgtcgcg
     1801 agccgccgtt ttatccgcta cgctatagaa gagcccgtgc gaccccgcgc cgagacgccc
     1861 gtggagcacc gcttccagcg ccgtgatggg aaagtcgcgg ttgcgccccg cgtcctgcgg
     1921 gacgtacccc gccgcgctcc ggtcctcgcc gggcgccctc ccgtggatca gcaccgtccc
     1981 cgaaccgggg gagagcagtc cgagcgcgag cttcagcagg gtggtctttc ccccgccgtt
     2041 cggccccatg atcaccagaa attcgcctgc gggaacggaa aatgatacgt cctgcagaac
     2101 agggtcgcct ccgtaggaaa aagcgacgtt cgtaaaggag agagccgact ccgtatgcac
     2161 tcgttttatg ccttctttcg tttcgtattc cgcggttcgc cgggcttctg cctcgcggcg
     2221 cgcccatcca gcgctttgcg gatgccttcc gtcaccgtgg tcagtatctt aatgcgcgcc
     2281 atctttttgg agttcgtcgc cacgacggtc cacggcgcgt agggcgtgcc ggtgcgcacc
     2341 agcatctctc cggctgcctt ctggtagtcc tcccacttct cgcggttgcg cca
//
LOCUS       DAOTIL010000397         2387 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminobacteriaceae bacterium isolate AMR_MDS_3026
            NODE_52737_length_2387_cov_3.590909, whole genome shotgun sequence.
ACCESSION   DAOTIL010000397 DAOTIL010000000
VERSION     DAOTIL010000397.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868050
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminobacteriaceae bacterium (wastewater metagenome)
  ORGANISM  Aminobacteriaceae bacterium
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae.
REFERENCE   1  (bases 1 to 2387)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2387)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:19:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,224
            CDSs (total)                      :: 3,198
            Genes (coding)                    :: 3,184
            CDSs (with protein)               :: 3,184
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2387
                     /organism="Aminobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_52737_length_2387_cov_3.590909"
                     /isolate="AMR_MDS_3026"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:3034609"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2229)
                     /locus_tag="P5201_08640"
     CDS             complement(<1..2229)
                     /locus_tag="P5201_08640"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: protein motif:HMM:NF012403.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HRV98643.1"
                     /translation="MKALHISLLNGNPVVWCEGGSLFDALEAIGFPLPRSAPLSAMTL
                     WLPGRKGVPFPSSSLLGEAEGRGKVALVPFRTQGRSPSTFAEAAALAYRFEGSSPRGS
                     GVLFGSSVRWFAAAMRAVRLLTAGESVLPAAARTEGVWEARWIPAPDDSAQRLLEELA
                     ASMPHVCRSIASDEQGPPDHSPRRVLAAFVGRFVDLAMRGEERRKEEAPKGRKKKTAS
                     SDAGSLHDAWREALSGADARILWSDENELSEFARALSAWRRPVEFASKAAYRFCFRLE
                     EPEESNDWRLVYLVQPKADPSLLLPLDALWEQTPPPKKSRKKGPAPSPLPAVRDVSAE
                     FLLTMLGQAAALSPRVAQSLKQPAPSSIRMSSEEAFSFLGGEAQTLADAGFQMMLPRW
                     FVKGPGERIKVKAVVKAPTMQSASGMSLESVMEFDYALSLAGETLAPEELEMLVMLKG
                     DFVRFRGQWMKVDRESIASGLRLFSQKKETRATGRDLLMKAVGGEGALSGVSVDSAEL
                     HGWPAEILAKLVEKRSDDLPSPEGLRGVLRPYQQRGYSWLAFLKEWGLGACLADDMGL
                     GKSVQALALVQRERERGETRPALLVCPTSVMDNWRREAEKFTPGLSVAVHWGASRARR
                     GDFAGVAAANALVVSSYALVQRDIAFLKEVEWAGVLLDEAQNVKNPEAKQSRAVRSLR
                     SGYRVALTGTPVENHVGDLWSLMDFLNPGLLGSASAFKRRFHTPITRDGDTEAAERLR
                     RAT"
BASE COUNT          421 a          791 c          776 g          399 t
ORIGIN      
        1 cgtcgcgcgg cgcaggcgtt ccgcggcctc cgtgtcgccg tctctggtga tgggcgtgtg
       61 gaagcgccgc ttgaacgcgc ttgccgagcc gaggagcccc gggttcagga agtccatcag
      121 ggaccagaga tcgccgacgt ggttctccac tggcgttccg gtcaaggcaa cgcgataccc
      181 cgagcggaga gagcgcaccg cgcgggactg cttcgcctcg gggttcttca cgttctgcgc
      241 ctcgtcgagg agcactcccg cccactccac ctctttgagg aatgcgatgt cccgctggac
      301 gagggcgtag cttgaaacga ccagcgcgtt cgccgcggcg acgcccgcga agtcgccgcg
      361 gcgcgcgcgc gaagcgcccc agtggaccgc gacggagagt ccgggcgtaa acttctccgc
      421 ctctctcctc cagttgtcca tcaccgaggt ggggcagacg aggagcgccg gacgggtctc
      481 tccccgttcg cgctccctct ggacgagggc cagggcttgt acgcttttcc cgagccccat
      541 atcgtcggcg agacaggcgc cgagccccca ctccttcagg aaggcgagcc acgagtaccc
      601 ccgttgctgg taggggcgaa ggacgccgcg tagtccttcc ggagagggga gatcgtcgct
      661 cctcttctct acgagcttcg cgagtatctc cgcaggccag ccgtgcagct ccgcggaatc
      721 aacggagacg ccggagagcg ccccctcgcc gcccaccgcc ttcatgagca gatcgcggcc
      781 ggtagcccgc gtttctttct tttgagaaaa gagacggagc ccggaagcaa tgctctcgcg
      841 gtccaccttc atccattgac cgcggaaacg gacgaaatct cccttgagca ttacgagcat
      901 ctccagctct tcgggcgcga gcgtctcgcc ggcgagcgag agcgcgtagt cgaactccat
      961 cacgctttcg agcgacatcc ccgatgcgct ctgcatggtg ggcgctttca ccaccgcctt
     1021 taccttgatg cgttctccgg ggccttttac gaaccatctg gggagcatca tctggaaccc
     1081 cgcgtcggca agcgtttgcg cctcccctcc gaggaaggaa aacgcctcct cggaggacat
     1141 gcggatcgaa gagggagcgg gttgcttcag gctctgcgcg acccgaggag aaagagccgc
     1201 agcctgtccg agcatcgtga ggagaaactc cgcagagacg tctcggaccg cgggaagcgg
     1261 cgagggagcg ggccccttct tgcggctttt tttcggaggc ggagtctgct cccagagcgc
     1321 atcgagagga agaagaaggc tcgggtcggc cttcggctgg accaggtaga cgagccgcca
     1381 gtcgttgctc tcttcgggct cttcgaggcg gaaacagaag cggtaggcgg ccttcgaggc
     1441 gaattccacc gggcggcgcc atgccgaaag cgcgcgtgcg aactccgaga gttcgttttc
     1501 atcggaccag aggatccggg catccgctcc ggaaagagcc tctctccacg cgtcgtggag
     1561 gcttccggcg tccgacgacg ctgttttctt ctttctgccc ttcggcgcct cttccttccg
     1621 tctttcttcg ccgcgcatcg ccagatcgac gaagcgcccc acaaaggccg ccagaacgcg
     1681 ccggggagag tggtcgggcg ggccttgctc atcggaagcg atgctccggc agacatgggg
     1741 catcgaggcc gccagctctt cgagcagacg ctgggcggag tcgtcgggcg ccggaatcca
     1801 gcgggcttcc catacgccct ccgtccgcgc cgccgcaggg aggacggatt ctccggccgt
     1861 gagcagccgg acggctcgca tcgccgcggc gaaccacctg acggacgagc cgaagagaac
     1921 gcctgagcct cgcggagagc ttccctcgaa ccggtacgcg agcgcagccg cctccgcgaa
     1981 cgtagacgga gaccgcccct gcgtacggaa ggggacgagc gcgacctttc cgcgcccttc
     2041 cgcctctccc agcagagatg acgaggggaa cgggacgccc tttctccccg gcagccaaag
     2101 ggtcatggcg ctcagggggg ccgagcgggg aaggggaaat ccgatggctt caagcgcgtc
     2161 gaaaagagat ccaccttcgc accagacgac agggtttccg ttcaacagcg agatgtgcag
     2221 tgctttcaag gggttcagct cctcaagggc ggatggtcag gccgatctcc cggcagaagt
     2281 ccacaaggga cgtccaccgg tcggccgcga tggcgcgaag caccgacata tcaagttcgc
     2341 ggtattcatg gattgcgacg ttgcggaacc ccgtcatcgc aaccatc
//
LOCUS       DAOTIL010000398         2386 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminobacteriaceae bacterium isolate AMR_MDS_3026
            NODE_52759_length_2386_cov_4.200772, whole genome shotgun sequence.
ACCESSION   DAOTIL010000398 DAOTIL010000000
VERSION     DAOTIL010000398.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868050
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminobacteriaceae bacterium (wastewater metagenome)
  ORGANISM  Aminobacteriaceae bacterium
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae.
REFERENCE   1  (bases 1 to 2386)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2386)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:19:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,224
            CDSs (total)                      :: 3,198
            Genes (coding)                    :: 3,184
            CDSs (with protein)               :: 3,184
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2386
                     /organism="Aminobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_52759_length_2386_cov_4.200772"
                     /isolate="AMR_MDS_3026"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:3034609"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            121..441
                     /locus_tag="P5201_08645"
     CDS             121..441
                     /locus_tag="P5201_08645"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRV98644.1"
                     /translation="MNIPREPRTQCDVCSSLSESEYGYSKYGWPDHDVDLPDAAGSLV
                     MVKDLKPLSERKLQLWRCPGCGAWFLYTTDYEYLTNGTEDEQFLTRLSEEEAAEYLNR
                     PEAP"
     gene            complement(561..857)
                     /locus_tag="P5201_08650"
     CDS             complement(561..857)
                     /locus_tag="P5201_08650"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRV98645.1"
                     /translation="MYALTKEAYRRKIQARLGLAHAQAKLNELKEKNKTSSANPVGEY
                     SNEINELEKNIEETQKRLNELYEAGDEAWEHLDDTSERILNALRTAVKNAVAKQ"
     gene            complement(986..1207)
                     /locus_tag="P5201_08655"
     CDS             complement(986..1207)
                     /locus_tag="P5201_08655"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRV98646.1"
                     /translation="MDIVKIIAIGLIATGALGLTYGGFTYTQTTHVAGLGPIRFSVDG
                     NRTVYIPLWASGTAVALGGILLLFGGKLI"
     gene            1733..>2386
                     /locus_tag="P5201_08660"
     CDS             1733..>2386
                     /locus_tag="P5201_08660"
                     /inference="COORDINATES: protein motif:HMM:NF013757.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IclR family transcriptional regulator"
                     /protein_id="HRV98647.1"
                     /translation="MDQQGENAADKQGIRVLERLFDILSFLAERHDYAGIKEIAEATG
                     LSRTTVHRVLATCEENYVVLKDNIGRYRIGPRSLVWANSYQQQTGLAKIARQHLKELL
                     RDVGETVHLFIYEKGEAFYLEKMHTYNVTGLDSRIGSRLELYSTSAGRAILAALPEEE
                     FEAYLSSRELLPRTDWTVVDKEAFGKIIENARTKGYAEETQENEVGVRCVGAAILGKD
                     "
BASE COUNT          535 a          610 c          654 g          587 t
ORIGIN      
        1 ccctctcctc acggagctct ttcccggact gaaagaggag ctggaaaccg ggcgttttct
       61 cgctttcgga tggagttcga tcgcacgcga agcggacgcg attctcgccg gtgacgtcct
      121 atgaatatcc ctagggagcc ccgcacgcaa tgcgacgtct gttcgagtct ctcggagagc
      181 gagtatggat acagcaaata cgggtggccg gaccacgacg tcgatctgcc ggatgccgcg
      241 ggttctctcg ttatggtgaa ggatctcaag cccttgagcg agaggaagct gcaactctgg
      301 cggtgtccgg ggtgcggcgc gtggtttttg tacacaactg attacgagta cttgacgaac
      361 ggaacggagg acgagcagtt cctgacccgg ttgagcgagg aggaagccgc cgaatacctg
      421 aaccggccgg aagctccgta gtcctgacgg ttgctgttat cgcatcctcg ttgcctttgc
      481 aggctgtcgg ttacagacaa cggtttttta aacctcgtta aacgccctga acgggtgatt
      541 atcgaggctt caaagtatcc ttattgtttt gcgacggcat ttttcacggc ggtccgtaac
      601 gcgttcaata tccgctccga tgtgtcgtcg agatgctccc aggcctcgtc tccggcttca
      661 tacagctcgt tcaggcgttt ttgcgtttct tctatattct tttcaagttc gttgatttcg
      721 ttgctgtatt ctccaaccgg attcgctgaa gaagtcttgt ttttctcctt cagttcattg
      781 agtttggcct gcgcgtgcgc cagccccaat cgagcctgta tcttacgtct gtaggcttcc
      841 tttgtaagtg catacactac gccatctcct cttgttgcct cccaagttct ttccgatttt
      901 tcccggcgcg catgaaaaac aacgcccgtg accgggttcc aatctctttg ctgaatgcaa
      961 gagcggcatc tgccggaaag acagtttata tcagttttcc tccgaagagc agaagaattc
     1021 cgccgagagc gaccgcggtc ccgctcgccc aaagcggaat atagacggtc cgattcccgt
     1081 ccacggagaa tcggatcggc cccagtccag ccacatgggt tgtctgcgta taggtgaatc
     1141 ctccataggt cagccccagt gcgccggttg cgatcagccc tattgcaatg attttcacta
     1201 tgtccattcg actccctcct ccgggaaaaa tgcttcgatt tcgctgtgaa cgccgaaaga
     1261 attgcattgt cgtccgtgtg aaaatcggga agccggcgag cgacgttgtt gcgggacaac
     1321 ccgacgtgat ttcattgcct gtcggcgagt tgatcgcttc gtccttccgc tcacaacgaa
     1381 attattatat atcattttga aaaaacagtc aagtgaaaat ttaatattat atttgcattt
     1441 tacgcctatt tattattggt ttattcagta taaagtgaat tttttcaggc tgttttcagg
     1501 gctgtatgct gtattatgag acgcctcgct ctcagcgcgt ttttggtcgt cgaacgagcg
     1561 tatcggattt tggtattgta aaaatcaaaa aacaggggta taatgcccaa taagcggatt
     1621 ttaaaatccg cctgtcggaa taaagatgct gctttacgga taaaaagaag aaaaatttca
     1681 tcaggtggaa ccttgtcgaa gggaaagcag aggagtgtag agcgtgagaa taatggacca
     1741 acagggagaa aatgctgcgg acaagcaggg aatccgcgtt cttgaacgcc tcttcgacat
     1801 tctctccttc cttgccgaac ggcacgacta cgcggggata aaggagatcg ccgaagcgac
     1861 gggtctgtcg cggacaacgg tacatcgcgt tctggcgacc tgcgaggaga actatgtcgt
     1921 ccttaaggac aatatcggtc gctatcgcat cggtccgaga agccttgtct gggccaactc
     1981 ctaccagcag cagacggggc tggccaagat cgcgcgccag catctgaagg agctgcttcg
     2041 ggacgtcgga gagactgtac acctcttcat ctatgaaaag ggcgaggcgt tctatctgga
     2101 aaagatgcat acctacaacg tcacggggct tgattcgcga atcggatcga ggcttgaact
     2161 gtacagcacc tccgcgggga gggccatact ggccgcgctt ccggaggagg agttcgaggc
     2221 gtacctctct tccagagaac tgcttccgcg caccgactgg accgtcgtcg acaaggaggc
     2281 gtttgggaag atcatcgaaa acgcgcggac aaaggggtac gccgaggaga cccaggagaa
     2341 tgaggtcggc gttcgctgcg tcggcgcggc catcctgggg aaggac
//
LOCUS       DAOTIL010000399         2385 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminobacteriaceae bacterium isolate AMR_MDS_3026
            NODE_52804_length_2385_cov_2.872961, whole genome shotgun sequence.
ACCESSION   DAOTIL010000399 DAOTIL010000000
VERSION     DAOTIL010000399.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868050
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminobacteriaceae bacterium (wastewater metagenome)
  ORGANISM  Aminobacteriaceae bacterium
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae.
REFERENCE   1  (bases 1 to 2385)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2385)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:19:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,224
            CDSs (total)                      :: 3,198
            Genes (coding)                    :: 3,184
            CDSs (with protein)               :: 3,184
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2385
                     /organism="Aminobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_52804_length_2385_cov_2.872961"
                     /isolate="AMR_MDS_3026"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:3034609"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            191..1084
                     /locus_tag="P5201_08665"
     CDS             191..1084
                     /locus_tag="P5201_08665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013048401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HRV98648.1"
                     /translation="MTLYDFESAHESFLAWLLLERGLSRNTASAYSRDLHKWFAYCTT
                     NGILPFPPDEDGIARFQRFLVESEGSKASRQRTMAGLRTWMRFLQVEEVLEADVALPP
                     LPQREQRLPKILSEGEVDRLLNVCGGDDPLSQRDRALFEVAYGCGLRASEICSLRVGD
                     VDFAAKTLRTLGKSEKERAVPFLGEPAHRVRRYLEEARPRLAKEETGILFLSRNGMML
                     NREDIWRIIQKRGAEAGISKQRLHPHILRHSFATHLLRRGMDQKTLQELLGHSSIATT
                     DKYLHFDLELRDVYDASHPRA"
     gene            1117..1932
                     /locus_tag="P5201_08670"
     CDS             1117..1932
                     /locus_tag="P5201_08670"
                     /EC_number="2.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008712316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004731 - purine-nucleoside phosphorylase
                     activity [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="purine-nucleoside phosphorylase"
                     /protein_id="HRV98649.1"
                     /translation="MIEKVKAASAFLKERISSSPESAVILGSGLGSFADSLEGRTVIP
                     YEEIPHWPLSTAPGHGGRLVHGRLGDRELLVMQGRVHYYEGYPMEEVVFPVRVLAQLG
                     VKNLVITNASGGVDVSLSPGDIVLLEDHVNFMGANPLRGPDTPGWNDRFPDMTRCYDE
                     KFIAAFEAAGAAEGAPVRRGVYFAFSGPSFETPAEIRMARALGATVVGMSTVPEAITA
                     NAMGLRVCALSCVANAAAGLTMNRLSGQEVLDEMAKTSEKLIRLLNRFFKEMR"
     gene            1934..>2385
                     /locus_tag="P5201_08675"
     CDS             1934..>2385
                     /locus_tag="P5201_08675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008712314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidine phosphorylase"
                     /protein_id="HRV98650.1"
                     /translation="MFDMISFIERKRDGGAHTAEEIQEFVRGFASGAVPDYQASAWLM
                     AVFHNGLKGEELKEFTLALAHSGKILSFGDLRIVDKHSTGGVGDKATLLLVPLAAACG
                     VKVAKLSGPGLSFTGGTVDKLEAIPGFQMHLSSERFIEQVRRIGCAISG"
BASE COUNT          493 a          586 c          739 g          567 t
ORIGIN      
        1 cccggccttg attacggggg gcaaactgaa ggatgccaag acgtttgccg cgctctcgtg
       61 gttgatggcc gccggtgagg ttcactgagt ttcttgtgaa tgatccgcta actgttttgt
      121 tgcactctat tacagacgcc tgaggaaccg gtttttccgg ttcctttttt tatgctacaa
      181 tgtaactatt atgacattat atgactttga atccgcccat gaatctttcc ttgcgtggtt
      241 gttgctggag cgggggttga gccggaatac agcctccgcg tactctcgtg atcttcacaa
      301 gtggtttgcg tattgcacga cgaacggaat tcttccattc cctcccgacg aggacggaat
      361 tgctcgcttt cagcgttttc ttgtcgagag cgaggggagc aaagcttcgc gtcagcggac
      421 aatggcgggc ttgcgtacgt ggatgcgctt tcttcaggtc gaggaggtcc ttgaggcgga
      481 tgtcgcactt cccccgttgc cgcagagaga acagcgtctt ccgaaaattc tcagcgaagg
      541 ggaggtggac agactgctca acgtctgtgg aggcgatgat cctctttcgc aaagagaccg
      601 tgcgcttttt gaagtggcgt atggatgcgg actgcgggcg agcgagatct gttctctccg
      661 ggtcggggat gtggattttg ccgcgaagac gctcagaacg cttgggaaga gtgagaagga
      721 gcgtgctgtg ccgtttcttg gagaaccggc ccacagggtg cggaggtatc ttgaagaggc
      781 ccggccgcgt ctggcgaagg aggagaccgg tattctcttc ctttcgcgga acggtatgat
      841 gctgaacagg gaggatattt ggcgaatcat acagaagagg ggagctgaag cgggtatttc
      901 aaaacaacgt cttcacccgc atatcctccg tcacagcttc gcaacgcatc ttctccggcg
      961 cggtatggac cagaagacgc tccaggaact tcttgggcat tcctccatcg caacgaccga
     1021 taagtacctg cactttgacc ttgaacttcg ggacgtgtat gatgcttctc atccacgtgc
     1081 ttaaaacgaa aaaattaagg aagaggagtt gagagaatga tcgaaaaagt caaggccgca
     1141 agcgctttcc ttaaagaacg tatttcttcg tctccggaat ctgcggtgat tttgggttca
     1201 gggttgggct ctttcgcgga ttcccttgaa gggcgtaccg tgatacccta tgaagagata
     1261 ccgcactggc ctctttcaac cgctcccgga cacggaggaa gacttgtcca cggaaggctg
     1321 ggagatcgtg agctcctcgt aatgcagggc cgcgttcact attacgaagg atacccgatg
     1381 gaagaggtag tgttcccggt gcgcgtgctc gctcagttgg gcgtgaagaa cctggtgatc
     1441 accaacgctt cgggcggagt cgacgtctcg ctctcgccgg gggatattgt tcttctggag
     1501 gatcatgtca atttcatggg agcgaatccg ctgcgcggtc ccgatactcc ggggtggaac
     1561 gaccgtttcc cggatatgac gcgatgctac gacgaaaaat tcatagccgc tttcgaagcg
     1621 gcaggagcgg ctgagggtgc gccggtgcgc aggggagtct atttcgcttt ttcaggaccg
     1681 tcgttcgaga ctccggccga aatacggatg gcgcgcgcgt tgggggcgac cgtggtggga
     1741 atgtccaccg taccggaagc tattactgca aacgcaatgg ggctgcgtgt ctgcgcgctt
     1801 tcgtgcgtgg ccaacgctgc tgccggtttg acgatgaaca ggctgagcgg gcaggaggtg
     1861 ctcgacgaga tggcgaagac atccgaaaag ttgattcgcc tcttgaatcg ttttttcaag
     1921 gagatgagat gaaatgttcg atatgatttc gttcatcgag cggaaacgag acggcggagc
     1981 ccacacggcg gaagagattc aagagttcgt tcgaggattt gcctccgggg ccgtccccga
     2041 ctatcaggcc agcgcctggc tgatggcggt gtttcacaac ggattgaagg gggaagagct
     2101 caaagagttt accctcgcgc tggctcattc gggaaagatc ctctccttcg gagaccttcg
     2161 cattgtcgac aaacacagca cgggaggtgt gggcgacaag gcgacgcttc ttcttgttcc
     2221 tctggcggcg gcctgcggtg tgaaggtggc gaagctttca gggccggggc tgagttttac
     2281 aggcggcaca gtcgataaac tggaggccat ccccggtttt caaatgcacc tctcttcgga
     2341 gcgttttatc gaacaggtgc gccggatcgg ttgcgctatt tccgg
//
LOCUS       DAOTIL010000400         2378 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminobacteriaceae bacterium isolate AMR_MDS_3026
            NODE_53018_length_2378_cov_2.667241, whole genome shotgun sequence.
ACCESSION   DAOTIL010000400 DAOTIL010000000
VERSION     DAOTIL010000400.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868050
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminobacteriaceae bacterium (wastewater metagenome)
  ORGANISM  Aminobacteriaceae bacterium
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae.
REFERENCE   1  (bases 1 to 2378)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2378)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:19:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,224
            CDSs (total)                      :: 3,198
            Genes (coding)                    :: 3,184
            CDSs (with protein)               :: 3,184
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2378
                     /organism="Aminobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_53018_length_2378_cov_2.667241"
                     /isolate="AMR_MDS_3026"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:3034609"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..389)
                     /locus_tag="P5201_08680"
     CDS             complement(<1..389)
                     /locus_tag="P5201_08680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005659865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HRV98651.1"
                     /translation="MENKKLLLDIQDLSIQYDTEGGTVHAVEHLNLTLEHGENLGFVG
                     ETGAGKTTTALGIMGLIPNPPGIIADGKIFFEGEDLLKKSERQMRDIRGGKIAMIFQD
                     PMTSLNPVMTVDKQIAEMVLLHNDVSE"
     gene            complement(408..1280)
                     /locus_tag="P5201_08685"
     CDS             complement(408..1280)
                     /locus_tag="P5201_08685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013049038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HRV98652.1"
                     /translation="MSGNTAPRMKSEAGLLDIWRRLRKIPLAMFGLFIVVLLIFFAIT
                     ADVIAPYKYYEQNLINSFQSPSKEHLFGTDEFGRDILSRIIHGSRISLQVGLIAVGIS
                     VIFGGFFGAVAGYYGGRIDNLIMRGMDILLSIPSILLAIAIAASLGPGLYNMMVAVGI
                     STTPQYARIIRGSVLSLRGQEFVEAAKAVGSSDTRIIMKHILPNCLAPIIVQSTLGVA
                     SAILTAAGLSFIGLGIQPPTPEWGAMLSGGREYIRDYAYMTVFPGLAIMITILALNFL
                     GDGLRDVLDPKLKR"
     gene            complement(1294..2223)
                     /locus_tag="P5201_08690"
     CDS             complement(1294..2223)
                     /locus_tag="P5201_08690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008521396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HRV98653.1"
                     /translation="MLKYIVKRIFSLIPVLLGVAFIVFTLLYLTPGDPARIVLGEQAT
                     EEALAEFREKEGLNDPFLVQFGNYVWKAVTKGDIGRSYMTRRSVANEIMSAFPSTLKL
                     ALSCVIAAVLLGIPIGILSAIRQNTLFDNITRFLAIVGLSMPVFWQGVLLILLFSVKL
                     RWFPASGFDTWATLVLPTLTLACNPLAVITRMTRSSMLEVVRQDYIRTARAKGQTEQI
                     VIWRHALGNALIPIVTVIGLQFGSLLAGAVLTESIFSIPGVGRLMVDAIKMRDYPVVQ
                     GGVLFIAVAFSFVNLMVDLLYAWIDPRIRAQYR"
BASE COUNT          537 a          653 c          710 g          478 t
ORIGIN      
        1 tgctcgctga cgtcgttgtg gaggagcacc atctccgcga tctgtttatc caccgtcatg
       61 accggattca gcgaagtcat aggatcctga aagatcatcg cgatcttgcc gccgcggata
      121 tcgcgcatct ggcgttcgct tttttttaag aggtcttcgc cctcgaaaaa gatcttcccg
      181 tccgcgatta tgcccggagg attggggatc agccccatga taccgagcgc cgtagttgtt
      241 ttcccggcgc ccgtttcgcc tacaaaacca aggttttcgc cgtgttcgag cgtgagattg
      301 agatgttcga cggcgtgcac cgtgccgcct tccgtatcgt actgaatgct cagatcctgt
      361 atgtccagaa gaagtttctt gttttccata gtcgtcgcct ccgcctttta tcgcttcagc
      421 ttcgggtcga gcacgtcgcg cagaccgtcg ccgaggaagt tgagcgccaa tatggtaatc
      481 atgatggcaa ggccggggaa gaccgtcatg taggcgtaat cgcggatgta ctcgcgtccg
      541 ccggagagca tggcgcccca ctcgggcgtg ggcggctgaa tgcccaaacc gatgaaggag
      601 agaccggccg ccgtcaaaat ggcgctcgcc acaccgagcg tcgactggac gatgatcggc
      661 gcaaggcagt tggggaggat gtgtttcatg atgatccggg tgtcggaaga accgacggcc
      721 ttggctgcct cgacgaactc ctgccctcgc agcgagagca ccgagccccg gatgatgcgc
      781 gcatactgcg gcgtggtcga aatgccgacg gcgaccatca tgttgtacag tcccggcccg
      841 agcgaggcag cgatggcgat ggcgagaaga atcgatggaa tggaaagaag gatgtccatc
      901 cctctcatga tgaggttgtc gatgcgcccc ccgtagtatc cggcgacggc tccgaagaaa
      961 ccgccgaaaa tgaccgagat gccgaccgcg atcagtccga cttggagcga gatgcgcgag
     1021 ccgtggataa tgcggctgag aatatcgcgc ccgaattcgt ccgtgccgaa gagatgctcc
     1081 tttgaaggag actggaagga gttgatcaaa ttctgttcat aatacttgta cggcgcgatg
     1141 acgtcggctg taatggcaaa aaagatgaga aggacgacga taaaaaggcc gaacattgcc
     1201 aaagggattt tgcgaaggcg tctccagatg tccaggagcc ctgcctcgct cttcattcga
     1261 ggtgccgtat ttccactcat tttctttcct cccctacctg tactgcgctc tgattctcgg
     1321 gtcgatccag gcgtagagga ggtcgaccat gagattcacg aagctgaaag cgacggcgat
     1381 gaacaggacg ccaccctgaa cgacaggata gtcccgcatc ttgatggcat ccaccataag
     1441 gcgcccaact ccggggatgg agaatatgga ctcggtgagc accgcgcctg cgagcagcga
     1501 accaaactgg agaccgatga ccgtaacgat ggggataagc gcgttgccga gagcatgtct
     1561 ccagataacg atctgctccg tctgtccttt tgcccgcgcc gtgcgaatgt agtcctggcg
     1621 gaccacttca agcatgctcg aacgggtcat tcgcgtaatg acggcgagag gattacaggc
     1681 gagcgtaagg gttggaagca caagcgtcgc ccacgtgtcg aaaccggaag cggggaacca
     1741 gcgcagtttc acggagaaga gcagaatcag gagcacgccc tgccagaaga ccggcatgga
     1801 aagcccgaca atcgcgagga aacgcgtgat gttgtcgaag agcgtgtttt gacgaatggc
     1861 cgaaagaata cctatcggaa ttccgagcag cacagcggcg atcacgcagc tcagcgcgag
     1921 tttcagggtg gacgggaacg cgctcataat ctcgttcgcc acggaacggc gcgtcatgta
     1981 ggaacgcccg atatcaccct tggtgacggc cttccagacg tagttgccga actggacgag
     2041 gaacgggtcg ttcagcccct ctttctcgcg aaactcggcg agcgcttctt cggttgcctg
     2101 ctcgcccaac acgatgcgcg ccggatcacc cggcgtcagg tagagcagcg tgaagacgat
     2161 gaaggccacc ccgagcaaca ccgggatcag cgaaaaaatt cgtttcacaa tatacttcag
     2221 caatggacta tccctcctgg acatccccgc agcggcctgc gggaagcaag gcaaagcagg
     2281 agcggaacgc tcctgctttg ccggtagaat cggttatttt ttcttcttat tacttgaagg
     2341 tgactccggt aacattgtgg tagcctctgg ggcttggg
//
LOCUS       DAOTIL010000401         2362 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminobacteriaceae bacterium isolate AMR_MDS_3026
            NODE_53477_length_2362_cov_3.281318, whole genome shotgun sequence.
ACCESSION   DAOTIL010000401 DAOTIL010000000
VERSION     DAOTIL010000401.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868050
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminobacteriaceae bacterium (wastewater metagenome)
  ORGANISM  Aminobacteriaceae bacterium
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae.
REFERENCE   1  (bases 1 to 2362)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2362)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:19:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,224
            CDSs (total)                      :: 3,198
            Genes (coding)                    :: 3,184
            CDSs (with protein)               :: 3,184
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2362
                     /organism="Aminobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_53477_length_2362_cov_3.281318"
                     /isolate="AMR_MDS_3026"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:3034609"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..670
                     /locus_tag="P5201_08695"
     CDS             <1..670
                     /locus_tag="P5201_08695"
                     /inference="COORDINATES: protein motif:HMM:NF013757.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="IclR family transcriptional regulator"
                     /protein_id="HRV98654.1"
                     /translation="HAGIGKSGAYKLLRTMQEQNFVIQDHSSKKYHLSPIMLRLGNVY
                     GHFKGIGETAPPILAHTSKTVGETTYITIWEGDRAFPAYKHRVPGGVYDVNDFIGKSI
                     PLNSGGSAMLLCAYQDPEHIRSLLDASELERRTPYTLVDRDVLMEEYRKIRDQGYALE
                     DEVFNIGVLSLAVPVFDRERAVRISLAMAAPKKHATEGKIVEWLHLLKDGAEELSYKL
                     QFRR"
     gene            760..1779
                     /gene="dctP"
                     /locus_tag="P5201_08700"
     CDS             760..1779
                     /gene="dctP"
                     /locus_tag="P5201_08700"
                     /inference="COORDINATES: protein motif:HMM:NF037995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter substrate-binding protein DctP"
                     /protein_id="HRV98655.1"
                     /translation="MIRKSTACGVLGAFVLGLLLTGVFALPSAAADKLVLKVAGISSP
                     EYRGSKSLEAIKEIVEKGTEGRVVLDVFPANQLGDYTQVFEEIRRGTIEMGLIFLPSQ
                     FDPMLEVGSLPFLASTGEGMQKQLSPGSFVYSIIEESLEKLGVRLLRIYGDGFVGVGL
                     AKQPKNHAKAGVDKDAMIRVAPLAVYKETAEDLGFRTTSIPYADTYSGIQTGVCDGWI
                     GGSSQINYLSFRDVIKYYIPYNCLFDQTAYIVGKQVWEKISPEDRKVIVDAVNEQADK
                     SFADSNAEDIEYQKKLEEAGVEILPITPEEMAAIAEHVRATTWPKLEETYGKETIEKI
                     RESLK"
     gene            1834..>2362
                     /locus_tag="P5201_08705"
     CDS             1834..>2362
                     /locus_tag="P5201_08705"
                     /inference="COORDINATES: protein motif:HMM:NF016200.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter small permease subunit"
                     /protein_id="HRV98656.1"
                     /translation="MSFVETVRSSLVWKFLLKLQKALLVLSSCFVVLIMCVAVLLRYV
                     FKTDLFGIEEIVVIAAFWLYFIGSSYGVYDKSHVKADIIPQMLSARAQAFLSVMVNLT
                     IAALCDGLDVTKGSFYHHFTSMDGFARELLQYWEADGSARLLAALDGVDDPVERISVL
                     KELGITTNHDAESAIR"
BASE COUNT          503 a          666 c          729 g          464 t
ORIGIN      
        1 ccatgccggc atcggcaaga gcggcgccta taagctgctc cggacgatgc aggagcagaa
       61 cttcgtcatt caggatcact cctccaaaaa ataccatctc agccccatca tgcttcgcct
      121 cgggaacgtg tacggtcact tcaagggaat cggcgagacg gcccctccga tacttgcgca
      181 tacgtccaaa accgtcggcg agacaaccta tatcacgatc tgggagggcg accgcgcctt
      241 ccccgcctac aaacacaggg tacccggagg cgtgtacgac gtgaacgatt tcatcggcaa
      301 gagtattccg ctgaactccg gagggtccgc gatgctgctg tgcgcctacc aggatccgga
      361 gcatatccgc agccttcttg acgcttcgga gctggagcgc cgcacaccct acacgcttgt
      421 tgacagagac gttttgatgg aggagtaccg gaagattcgc gatcaggggt atgccctgga
      481 ggacgaggtc ttcaatatcg gggtgctttc actcgcagtt ccggttttcg acagggagag
      541 agcggttcgc atctctctcg ccatggctgc gcccaagaag cacgcgacgg aggggaaaat
      601 cgtcgaatgg cttcatcttc tgaaagacgg cgccgaggaa ctttcgtaca agctccagtt
      661 caggcgttga cgccgtgaaa ggaggagttg cggggaaaaa tgactgcgcg gaaaaggtct
      721 gtacaagtca ctaaagaatc tgaaaggagc gatgtcataa tgataaggaa aagtactgcg
      781 tgcggagttc tgggagcgtt tgttctcggg ttgctgctga cgggggtttt cgcgctgccg
      841 tccgccgcgg cggacaaact tgtgctgaag gtggctggga tctcctctcc ggagtaccgc
      901 ggaagcaagt cgctcgaagc catcaaggag attgtcgaga aggggacgga gggacgggtg
      961 gttctggacg tcttccccgc gaaccagctt ggcgactata cgcaggtttt cgaggagatc
     1021 cggcgcggca cgatcgagat ggggctcatc ttcctcccca gccagttcga ccccatgctc
     1081 gaagtcggct cgctcccctt ccttgcctcc acgggcgagg ggatgcagaa gcagctctct
     1141 ccggggtcct tcgtgtattc gatcatcgag gaatcgcttg aaaagctggg cgtgcgtctc
     1201 ctccggatct atggcgacgg gttcgtcggc gtcggtcttg ccaagcagcc gaaaaaccac
     1261 gcgaaggcag gtgtggacaa ggacgccatg atccgcgtgg cgccgcttgc cgtctacaag
     1321 gagacggccg aggacctcgg cttccggacg acctccatcc cctacgcgga cacgtacagc
     1381 ggcatccaga cgggcgtttg cgacggctgg atcggcggtt cctcccagat caactacctg
     1441 agcttccgcg acgtcatcaa atactacatt ccctataact gcctcttcga ccagacggcg
     1501 tacatcgtcg gcaagcaggt ttgggagaag atctcccccg aggaccgcaa ggtgatcgtc
     1561 gacgccgtga acgagcaggc cgacaagagc tttgcggaca gcaacgcgga ggacatcgag
     1621 taccagaaga agcttgagga ggcaggagtg gagattctgc cgatcactcc cgaggagatg
     1681 gcggcgattg ccgagcatgt tcgcgcgacg acgtggccta agcttgagga gacctacggc
     1741 aaggaaacca ttgagaagat ccgggaatcg ttgaagtaga aagactctgc gtgcggggcg
     1801 gcgacgcccc gcacagtact ggagggggtt gccgtgagtt tcgtcgagac cgtcagatcg
     1861 tctttggtct ggaagtttct cctcaaactg caaaaagcgc ttctcgtcct gagcagctgc
     1921 ttcgtggtac tgatcatgtg cgttgcggtg ctgctgcggt acgtattcaa gaccgatctg
     1981 ttcgggatag aggagatcgt ggtgattgcg gccttctggc tgtatttcat aggaagttca
     2041 tacggcgtgt acgacaagag ccacgtcaag gcggacatca ttccgcagat gctctctgcg
     2101 cgtgcccaag cctttttaag cgtgatggtc aacctgacga tcgccgcgct ctgcgacggg
     2161 ctcgacgtca ccaagggcag cttctaccac cacttcacga gcatggacgg cttcgcccgc
     2221 gaactgctcc agtactggga ggccgacggc agcgcccgcc tcctggccgc gctcgacgga
     2281 gtcgacgatc ccgtcgagcg gatctcggtg ttgaaggaac tcgggatcac cacgaaccac
     2341 gatgccgaga gcgcgatccg tt
//
LOCUS       DAOTIL010000402         2359 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminobacteriaceae bacterium isolate AMR_MDS_3026
            NODE_53569_length_2359_cov_3.064670, whole genome shotgun sequence.
ACCESSION   DAOTIL010000402 DAOTIL010000000
VERSION     DAOTIL010000402.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868050
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminobacteriaceae bacterium (wastewater metagenome)
  ORGANISM  Aminobacteriaceae bacterium
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae.
REFERENCE   1  (bases 1 to 2359)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2359)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:19:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,224
            CDSs (total)                      :: 3,198
            Genes (coding)                    :: 3,184
            CDSs (with protein)               :: 3,184
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2359
                     /organism="Aminobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_53569_length_2359_cov_3.064670"
                     /isolate="AMR_MDS_3026"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:3034609"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..566)
                     /locus_tag="P5201_08710"
     CDS             complement(<1..566)
                     /locus_tag="P5201_08710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013048075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HRV98657.1"
                     /translation="MSGGIKIRESRCRGCANCIKSCPTEALRVIDGLVRILPDLCIDC
                     GECIRSCKDKAITVNDDEWDLIKSREGLILMADPTFYVQVGAYSRPRLMKEALEHHGL
                     RDLSEWASLAFDLAAFASARLIRDAGDNLPYISTYCPAVLRLIQINFPELVGRILPVE
                     SPLETAVSIWRDETGAEESVTLVSPCPA"
     gene            complement(635..1069)
                     /locus_tag="P5201_08715"
     CDS             complement(635..1069)
                     /locus_tag="P5201_08715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013048074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anti-sigma regulatory factor"
                     /protein_id="HRV98658.1"
                     /translation="MAQEPYVEEYAILAGDFSAIGEASTKFKATLKMLGIPSDLVRRA
                     SVIAYEAEMNALIHGGGGVLRMTVYDDRLEIVARDEGPGIPDVSLAMQEGYSTATDEI
                     RELGFGAGMGLPNIDRNADSLLIETEIDKGTTLLATVIFPSP"
     gene            complement(1073..1438)
                     /locus_tag="P5201_08720"
     CDS             complement(1073..1438)
                     /locus_tag="P5201_08720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013048073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DRTGG domain-containing protein"
                     /protein_id="HRV98659.1"
                     /translation="MKLRDIASQIRAEVLSGEELLDDIDIECAYGADLMSDVLAFARP
                     GSLLLTGLTNIQIVRTAQMLDLPAVVFVRGKKPQEAAVKLASQIKMPILLCHMSMFES
                     CGILFQKGILPCHIPDRGV"
     gene            complement(1490..>2359)
                     /locus_tag="P5201_08725"
     CDS             complement(1490..>2359)
                     /locus_tag="P5201_08725"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRV98660.1"
                     /translation="AVLSFAGGREIAFLRPHLGEAGPVFLSAFGETANIYNGEGVPDT
                     MVFALDLFRDFRIAAFTSYAAELFPSESVLAVLGDKLDPALERYARNLGDMFFDRGFL
                     VAHFWIPGGGMDSFKMIESEAIASGAKALVSWAGSMVVRDVWRSTRRKSGAFEIWYGG
                     VPQPLLLSFNGVVTADQDYPVRADSALKSLGRDIWRRHNVTVKDPAIAGRAYAACQWL
                     FDAFKRAGSPVPAALAGMLPLAEGLVFGSQALSINPATHRPAERAVAFMIVEGREFHP
                     VKTLLVRGPDYLP"
BASE COUNT          509 a          716 c          688 g          446 t
ORIGIN      
        1 ttcgccggac agggggagac caatgtcacg ctctcctccg ctcccgtctc atctcgccaa
       61 atggaaaccg ccgtctccag aggggattcc acgggcagaa tacgcccgac gagctccggg
      121 aagttgatct ggatcaggcg aaggacggcc ggacagtagg tggaaatata ggggagattg
      181 tcgcctgcat cacggatgag gcgggccgag gcgaacgcgg caagatcgaa cgcgagcgaa
      241 gcccattccg aaagatcacg aaggccgtga tgctccagcg cttccttcat cagtctcggc
      301 cgcgaatagg ctccgacctg gacgtagaat gtcgggtcgg ccataaggat cagaccttcc
      361 ctcgacttga tcagatccca ttcgtcgtcg ttgaccgtta tcgctttgtc tttacagctc
      421 cggatgcact cgccgcagtc gatacaaaga tcgggaagaa ttcggacgag accgtcgatg
      481 acgcgaagag cctccgtagg gcaggatttg atacagttgg cgcatccgcg gcaccgtgat
      541 tcgcgaattt tgatccctcc ggacattgaa atcactcctt tttcgtcgag aacaccaaag
      601 acaaaatcga gagaaaaccg cgcaggggat gcagctacgg cgaaggaaag atgaccgttg
      661 cgaggagcgt cgttcccttg tcgatctcgg tttcgatgag cagggaatcg gcgttcctgt
      721 cgatgttggg cagccccatc cctgcgccga acccgagctc gcgtatctcg tcggtcgccg
      781 tcgagtaccc ctcctgcatc gcgagcgata cgtccgggat tccgggcccc tcgtcgcggg
      841 cgacgatttc gagacgatca tcgtacaccg tcatgcggag cactcctccg cctccgtgga
      901 tcagcgcgtt catctccgcc tcgtacgcga tgacggaagc cctgcggaca aggtcggagg
      961 ggatacccag cattttcaac gtcgccttga acttggtcga cgcctcgccg atcgcggaaa
     1021 aatcacccgc cagaatggcg tactcctcca cataaggctc ctgggccacg tgtcacaccc
     1081 ctctgtccgg tatatgacaa gggagaatgc ctttctgaaa caggatgccg cacgattcga
     1141 acatgctcat gtgacagaga agaatcggca ttttgatctg ggaggccagc ttgaccgccg
     1201 cctcctgcgg ctttttcccc cggacgaaga caacggccgg aaggtcgagc atctgcgcgg
     1261 tccgtactat ctggatgttg gtcaatccgg tcaggagcag cgatccgggg cgggcgaacg
     1321 caagcacgtc gctcatcagg tcggccccgt atgcgcactc tatgtcgata tcgtcaagca
     1381 gctcttcgcc gctgagcacc tccgcgcgta tctgagacgc aatatcacgc agtttcattc
     1441 tacttcctcc tctatgatta tcttgaacac ttccccggga caggggcctt tacggcaggt
     1501 aatccggtcc tctgacaagg agcgttttga cggggtgaaa ctctctcccc tccacgatca
     1561 tgaacgccac ggccctctcg gcggggcggt gtgttgcagg attgattgaa agcgcctgag
     1621 aaccgaagac aagcccctcg gccaagggca gcatacccgc cagggcggcc gggacgggag
     1681 agcccgcccg cttgaaggcg tcgaaaagcc attggcaggc cgcatacgcg cgccccgcga
     1741 ttgcggggtc ttttacggtg acgttatgcc gtctccagat gtccctgccc agcgacttca
     1801 gagccgaatc agcccgcacg gggtagtcct ggtcggccgt cacaacgccg ttgaaggaga
     1861 gaagaagcgg ttgcggaacg ccgccgtacc aaatctcaaa ggcgccgctc ttgcgacggg
     1921 tgctgcgcca gacgtccctg accaccatcg accccgccca ggagacgagg gctttggcgc
     1981 ctgaagcaat ggcttccgat tctatcatct tgaaggaatc cattcctccg ccgggaatcc
     2041 agaagtgcgc cacaaggaag cccctatcga aaaacatatc gccgaggttc ctcgcatacc
     2101 gctcaagcgc ggggtcaagc ttgtcgccga ggaccgcaag aacgctctcg gaagggaaga
     2161 gctccgcagc atacgaggtg aacgcggcga ttctgaagtc gcggaacaga tccagcgcga
     2221 agaccattgt atcggggacg ccttcgccgt tgtaaatgtt cgcggtttct ccgaaagcag
     2281 agagaaagac gggtcccgct tctccgagat gcggccggag aaaggcgatt tctctccccc
     2341 cggcaaagga aagcactgc
//
LOCUS       DAOTIL010000403         2358 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminobacteriaceae bacterium isolate AMR_MDS_3026
            NODE_53583_length_2358_cov_5.783326, whole genome shotgun sequence.
ACCESSION   DAOTIL010000403 DAOTIL010000000
VERSION     DAOTIL010000403.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868050
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminobacteriaceae bacterium (wastewater metagenome)
  ORGANISM  Aminobacteriaceae bacterium
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae.
REFERENCE   1  (bases 1 to 2358)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2358)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:19:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,224
            CDSs (total)                      :: 3,198
            Genes (coding)                    :: 3,184
            CDSs (with protein)               :: 3,184
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2358
                     /organism="Aminobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_53583_length_2358_cov_5.783326"
                     /isolate="AMR_MDS_3026"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:3034609"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(135..908)
                     /gene="istB"
                     /locus_tag="P5201_08730"
     CDS             complement(135..908)
                     /gene="istB"
                     /locus_tag="P5201_08730"
                     /inference="COORDINATES: protein motif:HMM:NF013828.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS21-like element helper ATPase IstB"
                     /protein_id="HRV98661.1"
                     /translation="MNETGLAVAALSSRMDELGLTFMSAGIETFLADARRNDMTLLDS
                     LKDLIEIEYAARRERTARTRLKLSGMPQLKRLEDFDLSWLKGGLPQNRFEELASLAFV
                     ERKENVLLLGPSGVGKTHLMLALGNKACISGYTAYYISCRDAIEKLLRAREQNRLRKT
                     LAWFRKPHVLMIDEVGHEPLTPEQAHVLFQLVNARYEVAPIIMTSNRSFAKWAEIMSD
                     EAVAVAMLDRLLHHSQVFSLKADSYRMKNRMKIGAVGFE"
     gene            complement(905..2182)
                     /gene="istA"
                     /locus_tag="P5201_08735"
     CDS             complement(905..2182)
                     /gene="istA"
                     /locus_tag="P5201_08735"
                     /inference="COORDINATES: protein motif:HMM:NF033546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS21 family transposase"
                     /protein_id="HRV98662.1"
                     /translation="MTKEHCMLKAKILQEQGKTQLEIAAELGVTDRTVRNYLKRYDTP
                     RCGRVRKSKLDAFKVFIDTVLEDNPFLNREILIERLRKRGYEGRISILRDYAAAVTRK
                     ITKAAAIRFETEPGRQAQVDWKEFGKQVVDGRETKLYAFVMVLGYSRRPFVRFTTSMD
                     QSTLLACHVLAFSSFGGVPCEILYDNMRTAFAPDPEGLWQPTKQLLALGAHYGFAPLR
                     CRVRRPETKGKVERTIGYLDNNFWPRMEGQDLTLAGLNAEVGNWIETISGKVIADLGE
                     SRAVRFAREKPELKPLPAVDFDVRQEIPLHVGREGTIRHEGNRYTVPPSQIGELVCLL
                     VHPLHRDVELRLPDGSIRRFTLAAAGSRARIEFPGDVSSHRQRWLDDRERMARRRRPR
                     NRPARADIHVETGSASVYDALSGGVDAFEGVPA"
BASE COUNT          472 a          655 c          730 g          501 t
ORIGIN      
        1 tggtatgggt atgtgaacaa caggccgctc gacctggtgg atccgagcgg gttggcgggc
       61 gtaaaagtgt caacaccgat ttagattttc cgcttttcac cgatttcaac tttccgcttt
      121 tcggtgctga tttctcactc gaagccaacg gctccgatct tcattctgtt cttcatgcga
      181 tatgaatcag ccttcaagct gaagacctgg gagtgatgaa ggagacggtc cagcatcgcc
      241 acggcgacgg cctcgtcgga catgatctcc gcccattttg cgaagctgcg attcgaggtc
      301 atgatgatcg gagcgacctc gtagcgcgcg ttgacaagct gaaagagaac atgagcctgt
      361 tcaggagtga gcggctcgtg cccgacttcg tcgatcatga ggacatgagg tttgcggaac
      421 caggctaacg ttttgcggag gcgattctgt tcccgggcgc gaaggagctt ctcaatcgca
      481 tcccggcagg agatgtagta cgccgtgtag cctgagatgc aggccttgtt acccaaggcg
      541 agcatgaggt gggtcttacc gactcccgaa ggcccaagga gtaggacgtt ttcctttcgt
      601 tcgacgaagg caagggaggc gagctcctca aagcgatttt ggggtaagcc gcccttgagc
      661 caggagagat cgaaatcctc aaggcgcttc agctgcggca tgccggatag cttgaggcgc
      721 gtccgagcgg tgcgctcgcg gcgtgccgca tactctatct cgatgaggtc cttaagggag
      781 tcaagaagcg tcatgtcgtt tctacgggcg tcggcaagga aggtctcgat gcctgcagac
      841 atgaaggtaa gccccagctc gtccatgcgc gaggaaagag ccgcaacggc taatccagtc
      901 tcgttcatgc cggtacccct tcgaacgcgt ccacgccccc ggaaagcgca tcgtagaccg
      961 aagcggaccc ggtctcgacg tgaatatcgg cgcgggctgg ccgatttcgc ggacgtcgac
     1021 gccgagccat acgctcccgg tcgtcgagcc agcgctggcg gtgcgacgat acgtcaccgg
     1081 ggaactcgat gcgggcccgc gagccggcgg cagccaaggt gaaacgccgt atactgccgt
     1141 ctggaagcct gagttcgacg tcccggtgga gcgggtggac gaggaggcac acgagctcgc
     1201 ctatctgcga tggaggcact gtatagcggt tcccttcatg acgaatagta ccttcgcggc
     1261 cgacatggag cgggatctcc tgccggacgt cgaaatccac cgcgggaagg ggcttgagct
     1321 ccggtttctc gcgagcgaag cgtacggccc ggctctcccc aaggtcggcg atgacctttc
     1381 cggagatcgt ctcgatccag ttccccacct cggcattgag gcccgcaagg gtgagatcct
     1441 gcccctccat gcggggccag aagttgttgt ctagataccc gatggtgcgc tccaccttgc
     1501 ccttagtttc cggcctacgg acccggcatc gaagcggggc gaacccatag tgcgccccga
     1561 gggcgagaag ctgtttcgtc ggctgccaca ggccctcagg gtcgggggcg aatgccgtac
     1621 gcatgttgtc gtagaggatc tcgcaaggca cgccgccgaa agaggagaag gccagtacat
     1681 gacaggcgag caacgtggac tgatccatcg aggtggtgaa cctgacgaag ggccgccgcg
     1741 agtagccgag caccatgacg aaggcataga gcttggtttc ccggccatcg acgacctgct
     1801 tcccgaactc cttccaatcg acctgggctt gtctgcccgg ctcggtctcg aagcggatgg
     1861 cggcggcttt ggttatcttc cgggtcaccg ctgcggcgta gtcccggaga attgagattc
     1921 gcccctcgta tcctcgcttc cggagtcgtt cgatcaggat ttcacggttg aggaagggat
     1981 tgtcttcaag taccgtgtcg atgaagactt tgaaggcgtc gagcttgctc ttcctgaccc
     2041 gtccacagcg cggcgtgtcg tagcgtttga ggtagttcct gaccgtacgg tcggtgacgc
     2101 cgagttctgc cgcgatctcc agttgtgtct ttccctgttc ttgaaggatc ttcgccttca
     2161 gcatacagtg ctccttggtc atgggtgtct cctggtggaa ggttgtttgg taactccatc
     2221 ctaccagaga cgcccatggc tttatgcacc gaaagcggaa agcctagatc ggtgaattcc
     2281 ggaaatttaa catcggtgat gacaaagaaa ccatggaatc agttcttttg ttggttgaag
     2341 aatatgggaa aaaagaaa
//
LOCUS       DAOTIL010000404         2350 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminobacteriaceae bacterium isolate AMR_MDS_3026
            NODE_53830_length_2350_cov_2.704575, whole genome shotgun sequence.
ACCESSION   DAOTIL010000404 DAOTIL010000000
VERSION     DAOTIL010000404.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868050
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminobacteriaceae bacterium (wastewater metagenome)
  ORGANISM  Aminobacteriaceae bacterium
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae.
REFERENCE   1  (bases 1 to 2350)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2350)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:19:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,224
            CDSs (total)                      :: 3,198
            Genes (coding)                    :: 3,184
            CDSs (with protein)               :: 3,184
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2350
                     /organism="Aminobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_53830_length_2350_cov_2.704575"
                     /isolate="AMR_MDS_3026"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:3034609"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..225)
                     /locus_tag="P5201_08740"
     CDS             complement(<1..225)
                     /locus_tag="P5201_08740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008710841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HRV98663.1"
                     /translation="MRILIASVVLVALLASGAFASDLNAYTIMPEKYASQVFEAFTAE
                     TGVKVNFMRFSSGEALARVVAEKNNPQVDMI"
     gene            complement(459..>2350)
                     /locus_tag="P5201_08745"
     CDS             complement(459..>2350)
                     /locus_tag="P5201_08745"
                     /inference="COORDINATES: protein motif:HMM:NF013069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HRV98664.1"
                     /translation="GVLVNNAILIIENIFRYRDMGYSPEEAAEKGTVEISLSVLAGTA
                     TNLGVFLPVAIMTGIAGQFLVQYAVTIVYATLFSLWVSLTLTPSMAARFKADAGVPRL
                     GRFLCGWWDWIYSGFETLFLFLLRRAIRHPVLTILVFAGLTFGVFKAGGFIGAEFMPR
                     TDDGAMTINITLPSNTPVWVTQARAKEIEDRIRETLPALKYIVSTVGASGRNQGTHKA
                     TIEAYFHNNVERPSTMDMADQLRPFVNAMPGVDATVSGFRKGMGGGKPIQIIVRGDEL
                     ETLYRIAETLRDRIKHVPGIVDAGIDVEMGKPELQILPVRWRLSQFGINMTTLSQVVY
                     GYLVGMDAGKFRQDGYEYDIKARINPEHAKDIFKVPELPIMTEYGLVPLKEFSEVQWR
                     DGPTEIVRKDRRKAVTVEADARYITVGEATENARRAIEGIELPPGYEIQLGGDAEFMA
                     ENFAELGQALLIAIVLTFIIVAAILESWFFAFVITLSVPLAALGVIPAMLMSGVSISL
                     FALIGLIMLVGLVVNNAIVVIDYAEVRRVEEGEPGSIAIEKACAIRLRTLFMAISTAV
                     ISMVPLAAGTGDGGSFRQPIAFVAIGGLIAGGIIALLVIPAIYSLYWGFKERRAARRA
                     AAGQL"
BASE COUNT          499 a          738 c          678 g          435 t
ORIGIN      
        1 gatcatgtcg acctgcggat tgttcttctc cgccaccacc ctggccagcg cctctccgga
       61 cgagaaccgc atgaagttca ccttgacgcc ggtctcggcg gtgaaggcct cgaagacctg
      121 cgacgcgtac ttctccggca tgatggtgta cgcgttcaaa tccgaggcga aggccccgga
      181 cgccaggaga gcaaccaata cgacggacgc gatgaggatt ctcattttcg acaacagaat
      241 catctccttt tgaggtatgt gaacgatgtg taacggaagt gcaacatcac gcttattatc
      301 tcgtgaagac gctcattctg caatacaaaa aacaatgaac gcggggacga agagagggaa
      361 cactgaacgt ccgccggatc ccgttaaaaa ttaagaaaag cgaggaccga tatcttccgg
      421 cccccgcttc atagaaaaac tgattgaatc gcgctttctt acagctgccc ggccgccgcg
      481 cggcgagccg ctctgcgctc cttgaaaccc cagtagagcg aataaatcgc cggaataacc
      541 agcagcgcga tgataccgcc ggcgatcaaa ccgccgatgg cgacgaaggc gatgggctgg
      601 cggaacgaac cgccgtctcc cgtgcccgct gcaagaggca ccatcgagat caccgccgtc
      661 gagatggcca tgaagagggt tcgtagacga attgcgcacg ctttttcgat cgcgatcgat
      721 cccggctcgc cctcctcaac gcggcggacc tcggcgtagt cgatcacgac aatggcgttg
      781 ttcaccacca ggccgacgag catgatcagg ccgatgagcg cgaagagcga aatggaaact
      841 cccgacatca gcatcgccgg gatgacgccg agcgcggcca gcggcaccga gagcgtgatc
      901 acgaaggcga agaaccacga ctccagtatc gccgcgacaa tgatgaaggt gagcacgatg
      961 gcaatcagca gggcctgccc gagttcggcg aagttctccg ccataaactc cgcgtcgccg
     1021 ccgagctgga tctcgtaccc gggaggcagc tcgataccct cgattgcgcg ccgggcgttt
     1081 tccgtcgctt cgcccactgt gatatagcgt gcgtcagcct ccacagtcac cgccttccgg
     1141 cggtccttcc tgacaatctc cgtcgggccg tcccgccact gtacttcaga gaactctttc
     1201 agcggcacga gcccgtactc cgtcatgatc ggcagctccg gcaccttgaa gatgtccttc
     1261 gcatgctccg gattgatacg ggccttgatg tcgtactcgt agccgtcctg ccggaacttg
     1321 cccgcgtcca tgccgacgag gtagccgtag accacctgcg acagggtcgt catgttgatg
     1381 ccgaactggg agaggcgcca gcgcaccgga aggatctgaa gctcgggttt ccccatctcc
     1441 acgtcgatac cagcgtcgac gatgcccgga acgtgcttta tcctgtcgcg aagcgtctcc
     1501 gcgatacggt acagcgtctc cagctcgtct ccccggacga tgatctgaat gggttttccg
     1561 ccgcccatcc ccttgcggaa tccgctgact gtcgcatcca cgcccggcat cgcgttcacg
     1621 aaggggcgca gctggtcggc catatccata gtggacggac gttcgacatt gttgtgaaaa
     1681 tacgcctcga tcgtcgcctt gtgcgttccc tggtttcgtc cggacgcgcc gacggtggaa
     1741 acgatatatt tcaacgccgg aagcgtttcc ctgatccggt cttcaatttc cttcgccctc
     1801 gcctgcgtca cccagaccgg agtattgctc ggcagcgtga tattgatggt catcgctccg
     1861 tcatccgtgc ggggcatgaa ttccgcgccg atgaacccgc ccgccttgaa gacgccgaac
     1921 gtgagtccgg cgaagacgag gatcgtcagc acaggatgcc ggatcgccct tcggagcaag
     1981 aagagaaaga gcgtctcgaa gcccgagtaa atccagtccc accatccgca gaggaaacgc
     2041 cccaggcggg ggactcccgc gtcggccttg aagcgcgcag ccatcgaggg ggtgagcgtc
     2101 agcgagaccc acagggagaa gagcgtcgcg tagacgatcg tcaccgcgta ctgcacaagg
     2161 aactgccccg cgatgccggt catgatggcc accggaagga agacgccgag gttcgtcgcc
     2221 gtcccggcaa gcaccgagag cgagatttcc acggttcctt tttccgccgc ttcctcgggg
     2281 gagtacccca tgtcgcggta gcggaagatg ttctcgataa taaggatggc gttgttcacc
     2341 aggacgccca
//
LOCUS       DAOTIL010000405         2348 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminobacteriaceae bacterium isolate AMR_MDS_3026
            NODE_53888_length_2348_cov_3.310510, whole genome shotgun sequence.
ACCESSION   DAOTIL010000405 DAOTIL010000000
VERSION     DAOTIL010000405.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868050
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminobacteriaceae bacterium (wastewater metagenome)
  ORGANISM  Aminobacteriaceae bacterium
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae.
REFERENCE   1  (bases 1 to 2348)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2348)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:19:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,224
            CDSs (total)                      :: 3,198
            Genes (coding)                    :: 3,184
            CDSs (with protein)               :: 3,184
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2348
                     /organism="Aminobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_53888_length_2348_cov_3.310510"
                     /isolate="AMR_MDS_3026"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:3034609"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..294
                     /gene="rplB"
                     /locus_tag="P5201_08750"
     CDS             <1..294
                     /gene="rplB"
                     /locus_tag="P5201_08750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014162803.1"
                     /note="one of the primary rRNA-binding proteins; required
                     for association of the 30S and 50S subunits to form the
                     70S ribosome, for tRNA binding and peptide bond formation;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L2"
                     /protein_id="HRV98665.1"
                     /translation="GELRLILQECMATIGQVGNEDFENVSFGKAGKTRWLGRRPMVRG
                     MVMNPVDHPMGGGEGKSKSHKHPVSPWGTPAKGYRTRKKKPSDKMIVRRRYEK"
     gene            308..589
                     /gene="rpsS"
                     /locus_tag="P5201_08755"
     CDS             308..589
                     /gene="rpsS"
                     /locus_tag="P5201_08755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007285290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S19"
                     /protein_id="HRV98666.1"
                     /translation="MARSRKKGPYVDQKLLRKIEDMNEGTAKKVMKTWSRRSTIVPAM
                     IGHTIAVHNGRIHIPIYISENMVGHKLGEFAPTRKFGGHAGQERSSRVR"
     gene            603..935
                     /gene="rplV"
                     /locus_tag="P5201_08760"
     CDS             603..935
                     /gene="rplV"
                     /locus_tag="P5201_08760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006583237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L22"
                     /protein_id="HRV98667.1"
                     /translation="MEAKAVARQVRVSPIKVRRVLCLIRGKKVGEALVALRYSPQKSA
                     KLVSKVLQSAVANAEHNFGMDTDKLRIVAATADQGPVMKRFRPVSMGRAHPYRHRTSH
                     VTVIVAER"
     gene            949..1668
                     /gene="rpsC"
                     /locus_tag="P5201_08765"
     CDS             949..1668
                     /gene="rpsC"
                     /locus_tag="P5201_08765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009388013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000312 - plastid small ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S3"
                     /protein_id="HRV98668.1"
                     /translation="MGQKVHPIGFRLGVASEWESKWFASGKEYAKNLHEDIALRKYIA
                     KKWENAGISRIEIERVGHVIRFTVWTSRPGVVIGRGGAEIQVMKDELQAKTGAKIMIN
                     VQEIKNPDVVSQLVAVGVASALERRVSFRRAMKQSIFRAMKGGAKGIKISCAGRLGGA
                     EIARTEWYNEGRLPLSTIRADIDYGFAEAKTMYGVIGVKVWIFRDKENERPAQAPRQG
                     RGPRQDRSRGDRAPESSRGRG"
     gene            1675..2097
                     /gene="rplP"
                     /locus_tag="P5201_08770"
     CDS             1675..2097
                     /gene="rplP"
                     /locus_tag="P5201_08770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008521529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L16"
                     /protein_id="HRV98669.1"
                     /translation="MLMPSRVKYRKPFRRPLKGKAKGGNYVAFGEYGLQTLDCAWITA
                     RQIEATRVAISRKMKKGGKIWIRIFPDHPFTKKPLETRMGKGKGAPEFWVAPVKRGRI
                     LFEIAGVPRDIVEEAFRTASHKLPVKVRLVAREGFGGE"
     gene            2100..2315
                     /gene="rpmC"
                     /locus_tag="P5201_08775"
     CDS             2100..2315
                     /gene="rpmC"
                     /locus_tag="P5201_08775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013048036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L29"
                     /protein_id="HRV98670.1"
                     /translation="MESKNLRDLTLDELNEKHKQYKEELFNLRFQNAIGQLKNTSRIK
                     DVRKTIARVLTIVREKQMAGDQSAGRR"
BASE COUNT          584 a          608 c          742 g          414 t
ORIGIN      
        1 ggtgagctcc gcctcatcct tcaggagtgc atggcgacga taggccaggt cggtaacgaa
       61 gactttgaga acgtgtcctt cggcaaggcc gggaagaccc gttggcttgg aagacgtcca
      121 atggttcgcg gtatggtgat gaaccctgtg gaccacccca tgggcggcgg cgaaggcaag
      181 agcaagtcgc acaagcatcc cgtctccccc tggggaacgc ccgcaaaagg ataccgcacc
      241 cggaagaaga agccgtccga taagatgatc gttcggcgcc ggtacgagaa gtaaggaggg
      301 acactgaatg gctcgttcca gaaagaaggg gccctatgtg gatcagaagc tgctccggaa
      361 gatcgaagat atgaacgaag gaacagcgaa gaaggtcatg aagacctggt cccgaagatc
      421 gacgatcgtt cccgccatga ttggccatac gatcgcggtg cacaacggac ggatccatat
      481 acccatctac atcagcgaaa atatggtcgg acataagctt ggcgaattcg cccccacccg
      541 caagttcgga ggacacgcgg gtcaggagcg ttcctcaagg gttcggtgag gaggtaatgg
      601 tgatggaagc aaaagccgtt gccagacagg tacgggtttc tcccataaaa gtacggcggg
      661 tgctctgcct tatccgcggc aagaaagttg gagaagcgct tgtcgctctc cgctacagcc
      721 cgcagaagtc ggcgaaactc gtaagcaaag tgttgcagag cgcagtggcc aatgcggaac
      781 acaacttcgg catggacacc gacaaactgc gtatagtagc agctacggcg gatcaggggc
      841 cagtgatgaa gcgttttcgt ccggtctcca tgggtagggc gcacccctac cgccaccgta
      901 cgagtcatgt gactgtgata gtcgcggaac ggtaaggagg gggaactcgt gggacagaaa
      961 gttcacccaa tcggattccg actgggagtt gccagtgagt gggaatccaa gtggtttgcc
     1021 agcggcaagg agtacgcgaa gaatcttcac gaggacatcg cgcttcggaa gtatatcgca
     1081 aaaaagtggg agaatgcagg aatctcccgc atcgagatag agcgcgtcgg gcacgtcatt
     1141 cgcttcacgg tttggacctc ccggcccggt gtagttatcg gacggggcgg cgccgagatt
     1201 caggtgatga aggacgagct ccaggcgaag acaggcgcga agattatgat caacgtccag
     1261 gagattaaga accctgacgt cgtgtcgcag cttgtggcag tgggcgttgc atccgcgctt
     1321 gagcgcagag tgtccttccg ccgtgcgatg aagcagtcca tcttccgcgc catgaagggc
     1381 ggagcgaagg gaatcaagat atcctgcgcc ggccgtttgg gcggagctga aattgcccgc
     1441 acggagtggt acaacgaagg ccgtctgcct ctttcgacga tccgcgccga catcgactac
     1501 ggattcgccg aggcgaagac aatgtacggc gttatcggcg tcaaggtctg gattttccgg
     1561 gacaaggaga acgagcgtcc tgcgcaggcg ccccgacagg gtcgcgggcc ccgtcaggac
     1621 cgttcccgcg gagatcgcgc cccggagtct tcccggggaa gggggtaact cgcgatgttg
     1681 atgcccagca gagtgaagta tagaaagcct tttcgccgcc cccttaaagg gaaagcgaag
     1741 ggaggaaact atgtcgcctt tggcgagtac ggtctccaga ccctcgactg cgcctggatc
     1801 accgcgcgac agatagaggc cacgcgcgtc gccatctcac gtaagatgaa aaaaggcggc
     1861 aaaatttgga tccggatctt ccccgatcat cctttcacca agaaaccctt ggagacgaga
     1921 atgggaaagg gaaagggcgc gccggaattc tgggtggctc ctgtgaagcg cggcagaatt
     1981 ctctttgaaa tcgcgggtgt tcccagagac atagtggaag aagcattccg caccgcatcc
     2041 cacaagctcc ccgtcaaggt gcgtcttgtg gcccgggaag gttttggtgg tgaatgaaca
     2101 tggagagcaa gaatttgcgc gaccttacac tcgatgaact gaacgagaag cacaaacagt
     2161 acaaggaaga gctgttcaac ctgcgttttc agaacgccat cggacagctg aagaatacga
     2221 gcagaatcaa ggatgtacgt aagacgatcg cgcgggtatt gacgatcgtc cgcgagaagc
     2281 agatggccgg cgatcagtcc gccggaagga ggtagccccg aatggaagag cggaaacctc
     2341 accgcaag
//
LOCUS       DAOTIL010000406         2348 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminobacteriaceae bacterium isolate AMR_MDS_3026
            NODE_53891_length_2348_cov_3.126036, whole genome shotgun sequence.
ACCESSION   DAOTIL010000406 DAOTIL010000000
VERSION     DAOTIL010000406.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868050
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminobacteriaceae bacterium (wastewater metagenome)
  ORGANISM  Aminobacteriaceae bacterium
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae.
REFERENCE   1  (bases 1 to 2348)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2348)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:19:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,224
            CDSs (total)                      :: 3,198
            Genes (coding)                    :: 3,184
            CDSs (with protein)               :: 3,184
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2348
                     /organism="Aminobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_53891_length_2348_cov_3.126036"
                     /isolate="AMR_MDS_3026"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:3034609"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..328
                     /locus_tag="P5201_08780"
     CDS             <1..328
                     /locus_tag="P5201_08780"
                     /inference="COORDINATES: protein motif:HMM:NF028007.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051219 - phosphoprotein binding [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="FHA domain-containing protein"
                     /protein_id="HRV98671.1"
                     /translation="AQRASLHVELTPSGARGTDTTLKAVITDRIVLGRNRGPSALSIL
                     GDATVSARHCELVFSKGRLFVNDLQSSNGTMVNGVPIQGHYPLEDGDRLTIGKTELRI
                     RILEVK"
     gene            368..1183
                     /locus_tag="P5201_08785"
     CDS             368..1183
                     /locus_tag="P5201_08785"
                     /inference="COORDINATES: protein motif:HMM:NF012708.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051219 - phosphoprotein binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FHA domain-containing protein"
                     /protein_id="HRV98672.1"
                     /translation="MHYIALTGGGMLIVGCLLPWISLGTILVQQAPDIVFVALALLSG
                     LLATGVAIHNIVKKQNRMKYAYLAIGGICLMIGLYGLLRVRDIRTTAEFANVLQKRIT
                     AEADMPVATGIINTGLWFVAIGGLILFGTGLVNVFSQRMHAAGRGASGPRGGANGGLP
                     TFASGAQRAAILNGIAGPYAGRQIPVPLEGIIIGRDPAYSNLVIQSPAVSRRHARIMQ
                     GPSPDSCTLEDLNSTNGTFVMERNAWVRLTAPAVLTIFKRFRLGDEHTEFEIR"
     gene            1196..2035
                     /locus_tag="P5201_08790"
     CDS             1196..2035
                     /locus_tag="P5201_08790"
                     /inference="COORDINATES: protein motif:HMM:NF025053.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein phosphatase 2C domain-containing
                     protein"
                     /protein_id="HRV98673.1"
                     /translation="MMIRPGNYQHIGARKEQQDAFGFSDLSDVAFVRHGGVLAIVADG
                     MGGLQEGAEASRIAVRAFLESYGGKNTDERIEDALDRSLREANNSVYAFSVERNLEGN
                     CGTTIVAVVVHESGVYWTHAGDSRLYMTDGQSLTPLTEDHIYANNLKKAVEKGLLRQE
                     DALVHPERDALTSYIGDRTIKIVGKGFLKSSPDGLPPNGFMLLLCSDGLYKTLSEKEI
                     LEVYSPDPEEWARALVKSAIAQKYRFQDNVTVLCLAAGALVPRRSEDTLNNSHAIESD
                     DVE"
     gene            2094..>2348
                     /locus_tag="P5201_08795"
     CDS             2094..>2348
                     /locus_tag="P5201_08795"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRV98674.1"
                     /translation="MSTSNGAQEQQEISTPSPGQPLCPACFSPSAGSDVPCPVCGYSS
                     SQPEEGGWNRLKPGTIIHGRYIVGQPLGQGGFGITYEADDT"
BASE COUNT          571 a          702 c          640 g          435 t
ORIGIN      
        1 agcgcagagg gcttcactgc atgttgagct gacaccttcc ggagccaggg gaactgacac
       61 gactctcaag gccgtcatca ccgacaggat agtcctcggc agaaacaggg ggccgtccgc
      121 gctctccata ctcggcgatg caaccgtctc agcaaggcac tgcgaactcg ttttttccaa
      181 aggcagactc ttcgtgaacg acctccagtc ctccaacgga acgatggtca acggcgtccc
      241 catacaggga cactaccctt tggaggacgg cgacagactg acaataggga agacagagct
      301 tcgcatccgc attcttgagg tgaaataacc gttttttcgt acactatcgc aagggagatg
      361 acgcatcatg cactacatcg cgttgacagg aggaggaatg cttattgtcg gctgcctcct
      421 gccctggatt tcgctgggaa cgattctcgt tcagcaggcg ccggatatcg tcttcgtagc
      481 gctcgcactt ctctctggac tcctcgcgac gggggttgcc attcacaaca tagtaaaaaa
      541 gcaaaacagg atgaaatacg cctacctggc catcggcggc atctgcctca tgatcggcct
      601 ctacggcctc ctccgtgtac gtgatattcg cacaacagca gagttcgcca atgtactgca
      661 aaaacggata accgcagaag cagatatgcc ggtcgcgacg ggaatcataa acaccggcct
      721 ctggttcgtc gccattgggg ggcttatact cttcggaaca ggcctcgtca acgtattctc
      781 ccagaggatg cacgctgccg gaaggggcgc atcaggcccc agaggaggag cgaacggcgg
      841 ccttccgacc ttcgcctcgg gcgcacagcg ggcggccata ctcaacggaa tcgccggacc
      901 gtacgcaggc cggcagatcc ccgtgcccct cgaaggcatt ataatcggaa gagatccggc
      961 gtactccaat ctcgtcatcc agtcgcccgc ggtttcccga agacacgccc gcataatgca
     1021 gggcccctcc cccgacagct gtacgctgga agatttgaac tccacaaacg gcacttttgt
     1081 tatggaaaga aacgcttggg tgcgactgac cgcgcccgcc gtccttacca tcttcaagcg
     1141 cttccgactg ggagacgaac acacggaatt cgaaatccgc taggagccgc cgagaatgat
     1201 gatccgccct gggaactacc agcacatcgg cgccagaaaa gaacagcagg acgccttcgg
     1261 attcagcgat ctttctgatg tagctttcgt acggcacgga ggagttcttg caattgtagc
     1321 cgacggaatg ggcggactcc aggaaggagc ggaagcctcc cgtatcgcgg tacgcgcttt
     1381 cctggagtcg tatgggggaa aaaacacgga cgagagaata gaggacgctc ttgaccgctc
     1441 cctccgggag gccaacaact ccgtttacgc cttctccgtc gagagaaacc tcgaagggaa
     1501 ctgcggtact accatcgtcg ccgtcgtcgt ccacgaaagc ggcgtttact ggacgcacgc
     1561 tggagacagc aggctgtaca tgacagacgg gcagagcctg acgcctctca ccgaagatca
     1621 tatctatgcg aacaatctaa aaaaggctgt tgaaaaagga ttgctgagac aggaagacgc
     1681 gctcgtgcac ccggagagag acgccctcac aagctacatc ggcgacagga cgatcaagat
     1741 cgtcgggaag ggtttcctca aaagctctcc cgacggacta ccgccgaacg gctttatgct
     1801 tctcctctgc tcagacggcc tttacaaaac cctctccgag aaggaaatcc tcgaagtcta
     1861 ctcgccagac ccggaggaat gggcccgggc gctcgtcaaa agcgccatag cacaaaaata
     1921 tcgattccag gacaatgtga ccgtactctg ccttgccgca ggcgcacttg taccccgccg
     1981 gagcgaagac accctgaata actctcatgc cattgaatcc gatgatgtag aataagagca
     2041 gtatgtgcca tgacggcgcc gtgataaaaa gccgcacacc aggagtggag agcatgagta
     2101 catccaacgg agcacaagag cagcaggaga tttccacacc gagtcccgga cagcctcttt
     2161 gcccggcgtg tttctcccca tctgcgggat ctgatgttcc gtgccctgtc tgcggatact
     2221 cctcttctca acccgaagag ggcggatgga accgtctgaa accgggaacg atcatccacg
     2281 gccgctatat cgtcggacaa ccgctggggc aagggggctt cggcatcacc tacgaggccg
     2341 acgacacc
//
LOCUS       DAOTIL010000407         2344 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminobacteriaceae bacterium isolate AMR_MDS_3026
            NODE_54041_length_2344_cov_3.286151, whole genome shotgun sequence.
ACCESSION   DAOTIL010000407 DAOTIL010000000
VERSION     DAOTIL010000407.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868050
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminobacteriaceae bacterium (wastewater metagenome)
  ORGANISM  Aminobacteriaceae bacterium
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae.
REFERENCE   1  (bases 1 to 2344)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2344)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:19:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,224
            CDSs (total)                      :: 3,198
            Genes (coding)                    :: 3,184
            CDSs (with protein)               :: 3,184
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2344
                     /organism="Aminobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_54041_length_2344_cov_3.286151"
                     /isolate="AMR_MDS_3026"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:3034609"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            155..601
                     /gene="rplI"
                     /locus_tag="P5201_08800"
     CDS             155..601
                     /gene="rplI"
                     /locus_tag="P5201_08800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013048798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L9"
                     /protein_id="HRV98675.1"
                     /translation="MKVILKQDVNKLGASGDLVEISDGYARNFLFPRNLADEATPERM
                     KEWKDKEAARKKKEVRLEKEARELQKKLSGRAVKVKASIGEKGRLFGSITAANVAESI
                     VSQLAVEVEKKNLRMPESVKQIGSYSFTVRLHPGIEAEMTLIVEGE"
     gene            602..1954
                     /gene="dnaB"
                     /locus_tag="P5201_08805"
     CDS             602..1954
                     /gene="dnaB"
                     /locus_tag="P5201_08805"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012869407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replicative DNA helicase"
                     /protein_id="HRV98676.1"
                     /translation="MSVQSSWDRMPPVSLEAERSALGACLMDREALNIVMEILQPEDF
                     YDTTHRAAFEVIFGMAQRDKPVDPLTFLEEIARQGKGDLFGGQAFVGSLIDSVPTTAN
                     VEYHAGIVRDKAIHRRLISAGNTIVKLGYTEEMDVDEALEEAERAVFEIAQKRNSTNF
                     RHVAEVLGKTFQQIEEQYNRSDQDVTGFNSGFIELDRLTGGFQPGSLNIVAARPSMGK
                     TAFAVNIAQFGGQGAKRPVLIFSLEMGAEQLVQRMLGAEARINIHDLRNGSFPKSAWE
                     SLAEAAGRLAEAPIYIDDSSMLSTLEFRARCRRFKSKHKDLGLVLVDYLQLMNSSRRI
                     DNKQQEVAEISRALKGVARELEAPVIALSQLSRAVEQRNEKKPQLSDLRDSGAIEQDA
                     DTVILLYRPGYYDAGAPGEEEDNRAFISLAKHRNGPTGEISLIFLREYTRFVNADRRY
                     "
     gene            1964..>2344
                     /locus_tag="P5201_08810"
     CDS             1964..>2344
                     /locus_tag="P5201_08810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002649865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParB N-terminal domain-containing protein"
                     /protein_id="HRV98677.1"
                     /translation="MLEENAFYLYNSSEKLETWGAVFLTPAVADGIRTRGYSAPERSS
                     KIPGENDGMAGFLNFLRGDIAMNDGNEAAFSDTKAERRFADIPVDLIRPNPAQPRQFF
                     DEAELEELAASIAQVGVIQPIVLIA"
BASE COUNT          553 a          602 c          697 g          492 t
ORIGIN      
        1 cggagaaagc tcgcgtccct ttcgggcggg ttcttcgaac cgttgctata atagtagtag
       61 tgctggttcc tcttttttcc accatagcgg tcatggccgg aataggcgat atgtggctcg
      121 atttccggag acgtttcgga aggagtgatg cttcatgaaa gtcattttaa agcaagatgt
      181 aaataaactg ggcgcatccg gcgatcttgt tgagatttcg gatggatatg cacgtaattt
      241 cctgtttccg cgcaatcttg ccgacgaggc cactccggag cgaatgaagg agtggaaaga
      301 taaggaagcc gccagaaaga aaaaagaggt tcggctggaa aaagaggcgc gcgaactcca
      361 gaagaagctt tcagggcgcg ctgtgaaggt aaaggcgagt attggcgaaa aaggccgcct
      421 cttcggcagc atcacggccg ccaacgttgc ggagagcatt gtctcgcagc tcgcggtcga
      481 ggtggagaaa aaaaatctcc gtatgccgga gtctgtgaag cagataggaa gctattcttt
      541 taccgttcgc ctccatcccg gaatagaggc ggagatgacc ctcatcgtcg aaggcgagta
      601 gatgtccgtc cagtcttcct gggaccggat gccgccggtc tctcttgagg cggagcgatc
      661 cgcgctgggc gcctgcctca tggacaggga ggctctcaat atcgttatgg agattctcca
      721 gccggaggat ttttacgaca cgacccatcg agctgctttt gaggttatct tcggcatggc
      781 gcagcgcgac aaacccgttg accccctcac cttccttgag gagatcgccc gccaggggaa
      841 gggcgacctc ttcggagggc aggcgtttgt cggttcgctt attgacagcg ttcccaccac
      901 tgcaaacgtg gagtatcacg ccggtatcgt cagggataag gcgatccacc gcaggttgat
      961 cagcgcgggc aatacgatag taaaactcgg atacaccgag gagatggatg tcgacgaagc
     1021 actggaggaa gcggaacgcg ccgtcttcga gatagcccag aagcgcaact ccaccaactt
     1081 caggcatgtc gccgaggtgc tcgggaagac cttccagcag atagaggagc agtataatcg
     1141 ctccgaccag gacgtaacgg gcttcaactc cggttttatc gagctggacc gccttacggg
     1201 cggtttccag ccggggagtc tcaatatcgt cgcagcgcgc ccatcgatgg gaaaaacagc
     1261 atttgcggtc aacattgcgc agttcggggg acagggagca aagcgccctg tccttatctt
     1321 cagcctcgaa atgggcgccg aacagcttgt ccagcgaatg ctcggagcgg aggcgagaat
     1381 caacattcac gatctcagga acggctcttt tccgaaatcg gcatgggagt cgctcgccga
     1441 ggccgcaggg agacttgctg aggctcctat ttatatcgat gacagttcaa tgctctcgac
     1501 gctcgaattc cgcgcccggt gcaggcgttt caagtcgaaa cacaaggatc tcgggcttgt
     1561 cctggtagac taccttcagc tgatgaattc ctccaggagg atagacaata aacagcagga
     1621 agtcgcggag atttccaggg cgctcaaggg cgtggcccgt gaactggaag ctccggttat
     1681 cgcgctctcg cagctctcgc gtgctgtgga acagcggaac gagaaaaaac cgcagctttc
     1741 cgatctgcgt gacagcggcg caatcgaaca ggatgcggat actgtcatac ttctctaccg
     1801 ccccgggtac tatgatgcgg gagccccggg ggaagaagag gacaaccgtg cattcatcag
     1861 tctcgccaag catagaaacg ggccgaccgg cgagatctca ctgatctttc tccgggagta
     1921 tacccgtttt gtcaatgcgg accgccgtta ttaagcgttc cgtgtgcttg aagagaacgc
     1981 gttctatctg tacaatagtt cggagaagtt agaaacatgg ggcgccgtct tcttgactcc
     2041 ggccgtcgct gacggaatac ggacgcgggg atactccgcg ccggaacgga gctccaaaat
     2101 accgggggag aatgatggga tggctggatt tttgaacttt ttgagaggtg acattgcaat
     2161 gaacgacgga aacgaggcgg cgttctccga taccaaagcg gaaagacgat tcgccgatat
     2221 tccggtcgat ctcattcgac cgaatccagc gcaacccaga cagttttttg acgaggcgga
     2281 actggaggag ctcgcagcct ctattgcaca ggtaggcgtt attcagccga ttgtactgat
     2341 agct
//
LOCUS       DAOTIL010000408         2344 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminobacteriaceae bacterium isolate AMR_MDS_3026
            NODE_54046_length_2344_cov_2.824814, whole genome shotgun sequence.
ACCESSION   DAOTIL010000408 DAOTIL010000000
VERSION     DAOTIL010000408.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868050
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminobacteriaceae bacterium (wastewater metagenome)
  ORGANISM  Aminobacteriaceae bacterium
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae.
REFERENCE   1  (bases 1 to 2344)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2344)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:19:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,224
            CDSs (total)                      :: 3,198
            Genes (coding)                    :: 3,184
            CDSs (with protein)               :: 3,184
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2344
                     /organism="Aminobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_54046_length_2344_cov_2.824814"
                     /isolate="AMR_MDS_3026"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:3034609"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1530)
                     /locus_tag="P5201_08815"
     CDS             complement(<1..1530)
                     /locus_tag="P5201_08815"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HRV98678.1"
                     /translation="MHWKDSSTWKLFKYSLLSYLLLAGLFTMLYSLFAYSREKDEMER
                     TLYTVRRTHVPNIVDALWITDTQRVQRELDAIARFEFVDRAEVRDDEGRILFAGSEPL
                     LEAQLRTETLRYAHDDRSVVIGELSVFLGVRRMALKIINNALLFLLLQTALSVLLATI
                     AALAFHVSFGRPLSRLARFITADESSVSEKPFVLESRTHDRDELGILVSSFNRQHSAT
                     ARSIAEINEMNRRLSDANALLRQSEEALRTSKESAEWLGRQAESANRAKSVFLANMSH
                     ELRTPLNSVIGFLDLLGVTPLDEKQREYLGYVRTSAHSLLDVISNILDISKIETGKLE
                     LKPEFTNIRRLLEQAMNIARGTAVEKGLHLVLRVEDATPRRIFVDAIRLKQVLVNLLG
                     NAVKFTGKGEVELSLRFEETGNETGSFLFSIRDTGIGIPEEKQERLFEPFYQGDESIT
                     RQYGGTGLGLPICDALLQRMGSALELESVPGEGSRFFFTLAAGYADDEEAEEPFDASG
                     HA"
     gene            complement(1545..>2344)
                     /locus_tag="P5201_08820"
     CDS             complement(1545..>2344)
                     /locus_tag="P5201_08820"
                     /inference="COORDINATES: protein motif:HMM:NF012707.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HRV98679.1"
                     /translation="VFPVRGRPAEYQRQRGDVMFRSLAAVCLWIFLLLPAPETARAGE
                     RVFRISTSYKSLLSTPEQTGMLDMLLKEAFRRSGLRAEIVFTPTERSMYDVDAGLLDG
                     EINRIEGMEKRFPNLVRVPEPNMTMHFVAFSNRPYPVDGWESLKNLHVGVVSGWRILE
                     EHTVDFPKRILVPSETELFTMLHKGRIDVALYDKLTGYETISRLGFPEIRHLEPLLIN
                     RDMFLYMHKSHSEVVGTIAEALRAMKLDGAYDRIVSEATDHLGADAR"
BASE COUNT          439 a          689 c          718 g          498 t
ORIGIN      
        1 ggcgtgtccc gaagcgtcga agggttcttc ggcctcctcg tcgtcggcgt atccggcggc
       61 gagcgtgaag aagaagcggc ttccctctcc gggcacgctc tccagttcaa gcgcgcttcc
      121 catcctctgc aggagcgcgt cgcagatggg aagtcccagc cccgtccctc cgtactgtcg
      181 tgtgatcgat tcatccccct gatagaaggg ctcgaagagg cgctcctgtt tttcctccgg
      241 gatgccgatg ccggtgtccc ggatggagaa gaggaagctt cccgtttcgt tgcccgtctc
      301 ctcgaagcgg agcgagagct ccacctcccc tttcccggtg aacttgaccg cgttgcccag
      361 aaggttgacc agcacctgct tcaagcgaat cgcgtcgacg aagattcggc ggggcgttgc
      421 gtcctcgacc cggagcacga gatggaggcc cttctctacg gcggtacccc gtgcgatgtt
      481 catcgcctgt tcgagaagac gccggatatt cgtgaattca ggcttcagtt cgagcttgcc
      541 cgtttcgatc ttcgaaatgt cgaggatgtt gctgataacg tcgaggaggg agtgtgctga
      601 cgtcctgacg taccccaggt attcgcgctg tttctcgtcg aggggggtga cgccgagcag
      661 atcgaggaag ccgatcacgc tgttcagcgg tgtgcgcagt tcgtggctca tattcgcgag
      721 gaagacgctt ttcgccctgt tcgcgctctc ggcctgtctg ccgagccact cggcgctctc
      781 cttggacgtc cgcagcgcct cctccgactg acggaggagt gcgttggcgt cgctcagccg
      841 cctgttcatc tcgttgattt cggcgatgga gcgggccgtc gcgctgtgct gccgattgaa
      901 gctcgacacg aggatcccca gttcgtccct gtcgtgcgtg cgactctcca gaacgaacgg
      961 cttttctgac acagaacttt catcggcagt gatgaagcgc gccaagcggg aaagcggtct
     1021 tccgaaggag acgtgaaacg ccagcgcggc gatggtcgcg agcagaacgg aaagagctgt
     1081 ttgcagcagc agaaaaagca gggcgttgtt gatgatcttc agcgccattc tgcggacgcc
     1141 gagaaagacg gagagttcgc cgatgacgac ggagcgatcg tcgtgtgcat accgaagcgt
     1201 ctcggtccgg agctgcgctt caagaagggg ctccgatccg gcgaagagga tgcgcccctc
     1261 gtcgtcccgt acctcggccc tgtcgacgaa ctcgaagcgc gcgatcgcgt cgagttcgcg
     1321 ctgcactcgc tgcgtatccg tgatccagag tgcgtcgacg atgttgggaa cgtgcgtccg
     1381 gcgtactgta tagagcgtcc gctccatctc gtccttttcg cgcgagtatg caaagaggga
     1441 gtagagcatc gtgaagagcc ccgcgagcag caggtatgag agtaaggaat atttaaatag
     1501 cttccacgtg ctcgaatcct tccagtgcaa tggaactcgt cctttcaacg ggcgtccgcg
     1561 cccaggtggt cggtcgcctc cgagacgatg cgatcgtacg ctccgtcgag cttcatcgcg
     1621 cgcagcgctt cggcgatcgt tcccactact tcggaatggc tcttgtgcat atagagaaac
     1681 atatccctgt tgatcaggag aggctccaga tggcggatct ccggaaaacc cagacgggag
     1741 atcgtttcgt accctgtcag tttgtcgtag agagcgacgt cgatcctccc cttgtggagc
     1801 atggtgaaga gttccgtctc gctcggaacg aggattcttt tcgggaagtc cacggtatgc
     1861 tcttcgagaa tgcgccatcc gctgacgacg ccgacgtgca gatttttaag gctctcccat
     1921 ccgtccacgg gatacggccg attggagaag gcgacgaagt gcatggtcat gttgggttcg
     1981 ggtacccgca ccagattggg gaatcgtttc tccatcccct cgatccggtt gatctctccg
     2041 tccagcagtc ccgcgtcgac gtcgtacatg gagcgctccg tgggcgtgaa gacgatctcc
     2101 gcccgcaggc cggacctccg gaaggcctcc ttgagcagca tatccagcat tccggtctgt
     2161 tccggggtgg agagcaggct tttatacgat gtgctgatgc gaaagacacg ctcacccgcc
     2221 ctggctgttt ccggagccgg gagtaaaaga aaaatccaga ggcagaccgc tgcaagagac
     2281 ctgaacatga cgtcacccct ttgtcgttga tactccgccg gccggccccg tacggggaaa
     2341 acaa
//
LOCUS       DAOTIL010000409         2340 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminobacteriaceae bacterium isolate AMR_MDS_3026
            NODE_54161_length_2340_cov_2.381619, whole genome shotgun sequence.
ACCESSION   DAOTIL010000409 DAOTIL010000000
VERSION     DAOTIL010000409.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868050
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminobacteriaceae bacterium (wastewater metagenome)
  ORGANISM  Aminobacteriaceae bacterium
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae.
REFERENCE   1  (bases 1 to 2340)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2340)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:19:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,224
            CDSs (total)                      :: 3,198
            Genes (coding)                    :: 3,184
            CDSs (with protein)               :: 3,184
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2340
                     /organism="Aminobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_54161_length_2340_cov_2.381619"
                     /isolate="AMR_MDS_3026"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:3034609"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            66..467
                     /locus_tag="P5201_08825"
     CDS             66..467
                     /locus_tag="P5201_08825"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRV98680.1"
                     /translation="MPKGWTASLKATAARWRPGRGSKGLVATVTEKNRRPFAVSRRST
                     KGAGGAAVGDIPLVEPLVTESLPPIMFVRAIMVVGKESIAIMDINGVGNGIIVKTGYS
                     FLEKKGRILRITPEKVTVRWAGKNIDITPGF"
     gene            479..2224
                     /locus_tag="P5201_08830"
     CDS             479..2224
                     /locus_tag="P5201_08830"
                     /inference="COORDINATES: protein motif:HMM:NF012485.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="secretion protein"
                     /protein_id="HRV98681.1"
                     /translation="MKRPWKAFSVFMIFALLLAGCCVVHRPPVAEATTPPDAQALKLL
                     PPIGGLTVQQMGSHAVVMQLTGASLSRPEITSNSPDLIVFSVKPAYLASGKWERQFEL
                     PLVQGVYAEQKGEAVVFNVRINGTLVLKDIQGVAPANRYILRFDATGHAAREREEPAL
                     TAPKPAQPTNYNDPFTRTTPVTFDLRDVELRDVFRMLGEYVGMNIVADPSVPNTLVTM
                     SLKSVPLNEALGYILRMYDMGYAIMGKTIIVGAPDNIAKTTGKQSTRAFKIAYADMKT
                     VGGLVQSLAGVSNIVLDERLRTLYVTSRPEQFPHVERVLQEVDHPGRQVMLQARIIEV
                     TDAGRDQLETIIDAVYNQWWLNFSSGGMNLGYLYADKPGAYKPGDERPAPGIDDGLES
                     IAKGDLTKMLDFGLNSIVTANKGKVLADPSIITLDGKKAVIKLVENYPYISERDQAGN
                     PTWSEKEVGPILEFTPTVGRDQMVTVELKIQTGEIIGTYKGQAGEEFPQTSNREVTTM
                     IRVRDGEPFVVGGLHKDVKRTERGRVPILSNIPLLGELFKYRKDIRDKSEVAMIVIPY
                     ILTTPDGPVEQFTLK"
BASE COUNT          512 a          602 c          750 g          476 t
ORIGIN      
        1 ggtagttgat ctttccgtag aggaatccgt ttttgtccag cctcctccca ggcagtcgca
       61 ggaggatgcc aaaaggctgg acggcatcct tgaaagctac cgcggcacgg tggaggccag
      121 gcagggggag caaggggctg gttgcgaccg tgacggagaa gaaccgccgt ccgttcgcgg
      181 tttcccgcag gtcgacaaag ggggcgggcg gggcggcggt cggagatatt ccgcttgttg
      241 agccgcttgt gacggagtcg cttccgccga tcatgttcgt tcgggcgatc atggtggtgg
      301 ggaaggaatc catcgctatt atggatataa acggcgtcgg gaacggtatt atcgtaaaga
      361 cgggctattc gttcctcgaa aagaagggac ggatcctcag gattacgccg gagaaggtaa
      421 cggtccggtg ggccggcaag aatatcgata ttacaccggg attttagggg gaatcaggat
      481 gaagagacca tggaaggctt tttccgtttt tatgattttt gctctgcttc tagccggctg
      541 ttgcgttgtt catcgtcccc ccgtggccga agcgacgacg cccccggatg ctcaggcgct
      601 gaaactgctt cctcccatcg gggggcttac agtgcagcag atggggtcgc atgccgtggt
      661 tatgcagctt acgggcgcgt ctctttcgag gccggagatc acgagtaatt cgccggattt
      721 gatcgttttt tccgtcaaac cggcctatct ggcgtctggg aagtgggagc ggcagttcga
      781 gcttccgctg gtgcagggcg tatatgcgga acagaaggga gaggcagtcg ttttcaatgt
      841 ccgaatcaac ggaacgctcg ttttgaagga tatccagggc gttgccccgg cgaaccggta
      901 tatactccgt ttcgacgcca cagggcatgc tgcccgcgaa cgggaggagc cggcgcttac
      961 cgcgccgaag cccgcccagc cgacgaacta caacgacccg tttacgcgga cgactcctgt
     1021 gacgttcgac ctgcgcgacg tggagctgcg cgacgtcttc agaatgcttg gggaatacgt
     1081 gggaatgaac atcgtcgccg acccgtcggt cccgaacacg cttgtgacca tgtcgctgaa
     1141 gagcgttcct ctgaacgagg cgctcgggta tattctgcgc atgtacgaca tggggtacgc
     1201 catcatgggg aagacgatca tcgtcggcgc gcccgacaat atcgccaaga ccaccggaaa
     1261 acagtcgacg agggcgttca agatcgccta cgcggatatg aagacggtcg gggggcttgt
     1321 gcaaagcctt gccggagtct ccaatatcgt gttggacgag cgtctgcgca cgctctacgt
     1381 gacgagcagg ccggagcagt tcccgcatgt tgagcgcgtg ctccaggaag tcgatcatcc
     1441 ggggcgccag gtaatgctgc aggcgcgtat tattgaagta accgatgcgg ggcgcgatca
     1501 gctcgaaaca atcatcgacg cggtgtacaa ccagtggtgg ctcaacttca gctccggcgg
     1561 gatgaatctc gggtatctct acgccgataa gccgggggcg tacaaaccgg gcgatgaacg
     1621 tcccgcgccg gggatcgacg atggactgga gagcatcgcg aaaggcgact tgacgaagat
     1681 gctcgacttc gggctgaaca gcatcgtgac ggccaacaag gggaaggtgc ttgccgaccc
     1741 gtctattatc acgctcgacg gaaagaaagc cgtcatcaaa cttgtggaga actaccccta
     1801 catctccgag cgcgatcagg cgggcaaccc gacgtggagc gagaaggaag taggccccat
     1861 ccttgaattc acccccacag tggggcgaga ccagatggtg acggtcgagc tcaagattca
     1921 aaccggagaa atcatcggca cctacaaagg gcaggcaggg gaggagttcc cgcagacgtc
     1981 caaccgcgaa gtgaccacga tgatacgggt tcgcgacggc gagccgttcg ttgtcggcgg
     2041 cctgcataag gacgtgaagc ggaccgaacg cggacgtgtg cctatattga gcaacatccc
     2101 ccttctggga gagctgttca aatacaggaa ggatatccgg gacaagtccg aggtggcgat
     2161 gatcgtgatt ccgtacattc tgacgacgcc cgacggtcct gtggagcaat tcactttaaa
     2221 ataatgcgtt gtttttttgc agcggttttt tgagaaggac ttccggatat gataatatac
     2281 ctgcgttgtg gtgaatgcag cagctttgag tcgttttgaa ccgatattgg gaggtagagt
//
LOCUS       DAOTIL010000410         2334 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminobacteriaceae bacterium isolate AMR_MDS_3026
            NODE_54329_length_2334_cov_3.448004, whole genome shotgun sequence.
ACCESSION   DAOTIL010000410 DAOTIL010000000
VERSION     DAOTIL010000410.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868050
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminobacteriaceae bacterium (wastewater metagenome)
  ORGANISM  Aminobacteriaceae bacterium
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae.
REFERENCE   1  (bases 1 to 2334)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2334)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:19:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,224
            CDSs (total)                      :: 3,198
            Genes (coding)                    :: 3,184
            CDSs (with protein)               :: 3,184
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2334
                     /organism="Aminobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_54329_length_2334_cov_3.448004"
                     /isolate="AMR_MDS_3026"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:3034609"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            82..312
                     /gene="xseB"
                     /locus_tag="P5201_08835"
     CDS             82..312
                     /gene="xseB"
                     /locus_tag="P5201_08835"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006301078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009318 - exodeoxyribonuclease VII
                     complex [Evidence IEA];
                     GO_function: GO:0008855 - exodeoxyribonuclease VII
                     activity [Evidence IEA];
                     GO_process: GO:0006308 - DNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII small subunit"
                     /protein_id="HRV98682.1"
                     /translation="MSFSAKMDELEGLVARIEKEALPLEDALALFEKGVGLVRECREY
                     LSEAKQRILLLTEDSSTVPFVATYNNDEESSR"
     gene            309..1217
                     /locus_tag="P5201_08840"
     CDS             309..1217
                     /locus_tag="P5201_08840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013048249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HRV98683.1"
                     /translation="MTQNTRSFQALFSEAGEFVEARLQELAEMLPVPAPRRLRESMIY
                     SLTAGGKRLRPILCLKAAEVFGMSWDVSIPMALGFEMIHTASLIHDDLPAMDNDVLRR
                     GKATNHVLFGESLAILAGDALMAWAFEYPLSALSKSNVPAERICEAMRILANSLGPFG
                     ICGGQVLDTDPESMETGSDFPFKVAARKTAVLIAACVESGAVLGGADQAAANAFRTYG
                     NHLGLAFQMVDDILDVTSSAEELGKTPGKDATQNKKTFVSLFGLEETRRLAHNETEKG
                     INALRTIEGDTGFFAALSENLVERTR"
     gene            1220..>2334
                     /locus_tag="P5201_08845"
     CDS             1220..>2334
                     /locus_tag="P5201_08845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013048248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008661 - 1-deoxy-D-xylulose-5-phosphate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0016114 - terpenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate synthase
                     N-terminal domain-containing protein"
                     /protein_id="HRV98684.1"
                     /translation="MPVLERMKHYSELKGYSKETLDTLAAELREYIVSVVLKTGGHMA
                     SSLGAVELILALLRSFDPEDDKILFDVGHQAYAYKILTGRRDRFPTLRQWGGISGFPK
                     RSESPYDHFDVGHSSTSLSAALGYAKARDVLGAGNHVVAVIGDASLLNGLAFEALNYT
                     KDSNTKVIYILNDNTMSISKRVGGFATHLAKLSSSTAYNSLKKAIKESCASLPKGQAL
                     ENVLGKLKDHIKSIVKPINIFDEMDINYWGPFDGHNIEEIENVLELAKKYDKPVLIHL
                     LTVKGKGYEKAEQEPTKYHGVPPCKAVAVPATVSRRSWSDAASSAVCALAERDKRVVC
                     LTAAMKCGSKLDTFAERFPDRFFDVGIAEGHMLTMAAG"
BASE COUNT          572 a          552 c          650 g          560 t
ORIGIN      
        1 gataaaacag aagaatttta gcacagaggg gtgcatggag caaatttttt ttgtagaatt
       61 cctcggggag gattggacaa aatgagtttt agcgcgaaaa tggatgaact agaggggttg
      121 gtagcccgca tagagaagga agccctgcct ttggaagatg ccctcgccct ctttgaaaag
      181 ggggttgggt tggtacggga gtgccgggaa tatctttcgg aagcaaaaca gagaattctt
      241 ctccttacgg aggatagcag taccgtacct tttgttgcga cctacaataa cgatgaggag
      301 tcaagccgat gacccagaat acacgttctt ttcaagcgct tttttcggaa gccggggagt
      361 ttgtcgaggc gcgcttgcaa gaacttgccg agatgcttcc cgtccctgcc ccccgcaggc
      421 tcagggaatc gatgatttat tcactgactg ccggtgggaa acgtcttcgt cccattctct
      481 gcctgaaggc cgcggaggtt ttcggtatgt cgtgggacgt gtccatacct atggcacttg
      541 gctttgagat gattcatacg gcctctctca ttcatgacga tctccccgct atggataatg
      601 atgttttacg gagagggaag gcaacgaatc atgtcctctt cggcgaatcc ctcgccatac
      661 tcgccggaga tgcgttgatg gcctgggcgt ttgaataccc tctctctgcg ctttcgaagt
      721 cgaatgttcc cgctgagaga atctgtgagg caatgcgcat attggcaaac tcattggggc
      781 cgttcggtat ttgcggcgga caggttctcg ataccgaccc cgagagcatg gagacgggaa
      841 gtgatttccc gttcaaggtt gctgcccgga aaaccgcagt ccttattgcc gcctgcgtgg
      901 aaagcggcgc cgtccttgga ggggccgacc aagcagccgc aaacgctttc cgtacgtacg
      961 gcaatcattt gggtttggcc tttcagatgg tcgacgatat tctcgatgtc acgtcctcgg
     1021 cagaagaact gggaaaaaca ccaggcaaag acgccactca gaacaaaaag acgtttgttt
     1081 cccttttcgg gcttgaagag actcgccggt tagctcataa cgagactgaa aaagggataa
     1141 atgcactccg tacgatagaa ggagacactg gatttttcgc ggctctctcc gaaaatcttg
     1201 tggaaaggac gcggtagata tgcctgttct ggagcgtatg aagcactatt cggagctgaa
     1261 ggggtactca aaagagactc tcgatacgct cgccgctgaa cttcgagagt atatagtctc
     1321 ggtagtttta aaaaccgggg gtcatatggc ctcttcgctg ggggcggttg agctcattct
     1381 tgcgctgctg cgttcctttg atcctgaaga cgataaaata ctcttcgatg tcggacatca
     1441 ggcgtacgcg tataaaattc ttactggccg cagggaccgt tttccgacgc tgcggcagtg
     1501 gggcggtatc agcggttttc cgaaacgttc cgaaagtccg tacgaccatt ttgacgtggg
     1561 gcatagcagc acatcgcttt ccgcagcgct tggttatgcg aaagcgagag atgtcctggg
     1621 cgcgggaaac catgtcgtag cggtgatagg agacgcatcg ctcctgaacg ggctggcatt
     1681 cgaggcgctc aactacacca aggattcgaa cacgaaagtc atctatatcc tcaatgacaa
     1741 tacaatgtcc atcagtaaaa gagtaggcgg ctttgccaca catcttgcga aacttagctc
     1801 gagcaccgcc tataattctt tgaagaaggc gatcaaggag tcctgcgcct cgctaccgaa
     1861 gggacaggct cttgagaatg tgcttggaaa gctgaaggat cacatcaaat ccatagtcaa
     1921 gccgatcaat attttcgatg aaatggatat caactactgg ggaccttttg acggacataa
     1981 tattgaagaa atagaaaatg tcctggagct tgcgaaaaaa tatgataaac cagtgctcat
     2041 tcatcttcta acagtgaagg gaaaagggta tgaaaaagcg gagcaggaac ccacgaagta
     2101 tcacggcgtt cctccttgca aagctgttgc cgttccggca accgtttctc gaagatcatg
     2161 gagcgacgct gcttcaagcg cagtgtgcgc tcttgcggag cgcgacaagc gggtggtctg
     2221 ccttactgct gccatgaagt gcggcagtaa gcttgacacg ttcgcggagc gtttccctga
     2281 tcgtttcttt gatgtaggca tagccgaggg gcacatgctc actatggcgg ctgg
//
LOCUS       DAOTIL010000411         2328 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminobacteriaceae bacterium isolate AMR_MDS_3026
            NODE_54544_length_2328_cov_2.689837, whole genome shotgun sequence.
ACCESSION   DAOTIL010000411 DAOTIL010000000
VERSION     DAOTIL010000411.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868050
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminobacteriaceae bacterium (wastewater metagenome)
  ORGANISM  Aminobacteriaceae bacterium
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae.
REFERENCE   1  (bases 1 to 2328)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2328)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:19:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,224
            CDSs (total)                      :: 3,198
            Genes (coding)                    :: 3,184
            CDSs (with protein)               :: 3,184
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2328
                     /organism="Aminobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_54544_length_2328_cov_2.689837"
                     /isolate="AMR_MDS_3026"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:3034609"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(134..>2328)
                     /locus_tag="P5201_08850"
     CDS             complement(134..>2328)
                     /locus_tag="P5201_08850"
                     /inference="COORDINATES: protein motif:HMM:NF014069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="HD domain-containing phosphohydrolase"
                     /protein_id="HRV98685.1"
                     /translation="TFSIGTFFSIVVLSFLFIFLVQGATTLYIRVSARLDEFARHGEN
                     VLQVTSLLLEKQVEQREEGLRAIVGGADMNDAGRDVLVAAVLDGNTVKRGIKGWLPDG
                     MLLPDELLTDEWVVNDLLDIYGRSLLTGVRTIQGKRVFAAIRLNMNELDVFGDHLLLP
                     VISDNLGQIVWMGATKEAPALSEHLKARGFILRKKIPDQRWAIALSHQGSRVMMRQEQ
                     LPYGFTFTIAYPLASLLISSLSGLAVAAMLFLFALLVILLLWALWNRGVYKSIATIAN
                     LADEMGDRLSQIDARDPLRGAETLFTLTKRFSGFKETFVRETNSFVRDLRNLFEVILR
                     QQEELTAFNEETEAMNQELEQVNKQLISREALWERTLEFSRAFARNQDVDAAISSTLD
                     TLRRDVKAFGVLISSVEGDSFRLTAWSGYNGELTNFVVSKDGIAASESIISRSPVWVE
                     DVRAHPGAHPVHPSVKSELLIPLFQAGEEEGVLEVAFDRPMRRDSFLIETLTPVASFL
                     GGLVHGEKMRRELKASYAYLAEKLQFVTGIYHDETEAHIARIGEYCKLLAREIGRDAS
                     EQEDIALFARLHDIGKLRIPYGILVKPGALDSDEFNQIKRHPQWGADILGDAAWLQMA
                     RNICLTHHEKWDGSGYPRGLRGEEIPWEGRVATIADIYDALRSSRSYKPAMSHELAVS
                     ILLEGDERVRPEHFDPKVLTAFRRISGKFEEIFDSNCDERDSPDCFLA"
BASE COUNT          422 a          725 c          762 g          419 t
ORIGIN      
        1 atggagcatc ggagggggga gcgtccgaag ggcccactcc ttggagagca cgtccgcgga
       61 aagtcccgcg gcgaaaaaaa gaaacggcgt caggcggttc atgatgtgcg aggcgcccgg
      121 gtcatcttct ccgctacgcg aggaagcagt ccggcgagtc gcgctcgtcg cagttggagt
      181 cgaagatctc ctcgaacttc ccggagattc gcctgaacgc ggtgagcacc ttcgggtcga
      241 agtgctccgg gcgcaccctc tcgtccccct caaggaggat actgaccgcc agttcatgcg
      301 acatcgcggg cttgtacgag cgggacgacc ggagcgcgtc gtagatgtcc gcgatggtgg
      361 ccacccgtcc ctcccaggga atctcctcgc cgcgcagtcc gcgggggtat ccgctgccgt
      421 cccacttttc gtggtgcgtc aggcagatat tcctcgccat ctgcagccag gccgcgtcgc
      481 cgaggatatc ggctccccac tgcgggtggc gcttgatctg gttgaactcg tccgagtcga
      541 gcgcccccgg tttcaccagg atgccgtacg gaatccgcag cttgccgatg tcgtgcagcc
      601 gggcgaagag ggcgatgtcc tcctgctcgc tcgcgtccct gccgatctcg cgcgcgagca
      661 gcttgcagta ctcgccgatg cgggcgatat gcgcctccgt ctcgtcgtgg taaatccccg
      721 tgacgaactg gagcttttcc gcgaggtagg cgtaggaggc cttcagctcg cggcgcatct
      781 tctctccgtg gaccagtccg ccgaggaagg aggccaccgg ggtgagcgtt tcgatgagga
      841 aggagtccct ccgcataggc cggtcgaagg cgacttcgag cacgccctcc tcctcaccgg
      901 cctggaagag cgggatcagc agctcgctct tgaccgaagg gtgcaccgga tgggccccgg
      961 ggtgcgcgcg gacgtcctcc acccagacgg gcgaccggga gatgatgctc tccgaggcgg
     1021 cgatgccgtc cttcgagacg acgaagttcg tgagctctcc gttgtagccg ctccacgccg
     1081 tcaggcggaa gctgtccccc tccacggagg agatgaggac gccgaaggcc ttcacgtccc
     1141 ttcgcagcgt atccagcgtg gaggagatcg cggcgtccac gtcctggttg cgggcgaagg
     1201 cgcgcgagaa ctccagagtg cgctcccaca gggcttcccg gcttatgagc tgtttgttca
     1261 cctgctccag ctcctggttc atcgcctcgg tctcctcatt gaacgccgtc agctcctcct
     1321 gctggcggag aatgacctcg aagaggttgc ggaggtcgcg gacgaaggag ttcgtctccc
     1381 ggacgaacgt ctctttgaag ccggagaagc gctttgtaag ggtgaagagc gtctccgcgc
     1441 cccgcagcgg atccctcgcg tcgatctgcg aaagccggtc gcccatctcg tcggcgagat
     1501 tcgcgatggt cgcgatgctt ttatacacgc ctctgttcca gagagcccag agaagcagga
     1561 tgacaaggag agcgaagaga aagagcatgg ccgcgacggc gaggccggag agcgacgaga
     1621 tcagcagcga tgcaagcggg taggctatgg tgaaggtgaa tccgtaggga agctgctcct
     1681 ggcgcatcat cacgcgggag ccctggtgcg aaagggctat cgcccatcgc tggtcgggaa
     1741 ttttctttct gaggatgaac cccctcgcct tcagatgctc ggagagcgcc ggggcttcct
     1801 tcgtcgcccc catccagacg atctgcccca ggttgtcgga aatcaccgga agaagcaagt
     1861 ggtcgccgaa gacgtccagc tcgttcatgt tgaggcggat cgccgcgaag acacgtttcc
     1921 cctgaatggt gcgcaccccg gtgaggaggc tgcggccgta gatgtcgaga aggtcgttga
     1981 cgacccattc gtcggtcagc agctcgtccg ggagcagcat cccgtccggg agccacccct
     2041 tgatgccgcg cttcacagta tttccgtcga gaacggcggc cacgaggacg tcgcgcccgg
     2101 cgtcgttcat atccgcgccg ccgacgatgg cgcgcagtcc ctcctcgcgc tgctccacct
     2161 gcttttcaag cagaagcgag gtcacctgga gcacgttttc gccgtgacgc gcaaattcgt
     2221 cgagccgcgc gctcaccctg atgtagaggg tggtcgcccc ctgaacgagg aaaatgaaaa
     2281 ggaagctgag gacgacgatc gagaagaacg taccgatgga gaaggtga
//
LOCUS       DAOTIL010000412         2326 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminobacteriaceae bacterium isolate AMR_MDS_3026
            NODE_54589_length_2326_cov_3.758697, whole genome shotgun sequence.
ACCESSION   DAOTIL010000412 DAOTIL010000000
VERSION     DAOTIL010000412.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868050
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminobacteriaceae bacterium (wastewater metagenome)
  ORGANISM  Aminobacteriaceae bacterium
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae.
REFERENCE   1  (bases 1 to 2326)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2326)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:19:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,224
            CDSs (total)                      :: 3,198
            Genes (coding)                    :: 3,184
            CDSs (with protein)               :: 3,184
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2326
                     /organism="Aminobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_54589_length_2326_cov_3.758697"
                     /isolate="AMR_MDS_3026"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:3034609"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1559)
                     /locus_tag="P5201_08855"
     CDS             complement(<1..1559)
                     /locus_tag="P5201_08855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005659382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux transporter outer membrane subunit"
                     /protein_id="HRV98686.1"
                     /translation="MKKPTILLMSLCLLALLSFGAYGADNAPAPKDTELETGDWTTLA
                     KRYPMSYLAAEDEVPEAPSPEILANWWNALGDETLTRLIQYSLENNRDLISARAKFRE
                     ARAALGISKSAVLPWLDNSNTLTRSKTGKSGSSTGSQIGPMDFYSLGVDASWEIDIFG
                     GQAQKIKAGKADLQAEFGALHNAWVTLSSEVALNYTSLRTLQKRLQVAESNLALQMDT
                     VELLQSQHDSGLKDGLALNQAKYTMEQTRSTIPPLRTSIEETMNRLAILVGQVPGSLE
                     EMLGEYRPLPEPEAVGLVGIPANSLRQRPDIYSAERRLAAQIARKKSAQRDLWPKFNL
                     FGSIGLESFTTGGLSLSDGISYGFGPSISWPIFHGGAIRSNIKIQTAKQEQLLAAYEQ
                     TVLNAVAEVRNALTSETQERERNQSLRRGVDAARTAFEIAEDKYKNGLTDFNNVIGAQ
                     SALLTLEEQYAISEGQMLSNIVRIFKVLGGGWAPLAAGGFPQPVDGDTAKTTESQISS
                     ESRSYLEQIRQ"
     gene            complement(1564..>2326)
                     /locus_tag="P5201_08860"
     CDS             complement(1564..>2326)
                     /locus_tag="P5201_08860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008708913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HRV98687.1"
                     /translation="VPLESIMTLKKTLAPRTLSRYNLCPTAAVTAFMAPGYSTGQGIA
                     AMEKLASALPEGYSLEWSGMTYQEREAGGQTGLILLIAVLFGYLFLVAQYESWTVPLG
                     VILSLPVALIGALAGIFIMGISLSIYAQLGILLLVGLAAKNAILIIEFAKEQHDVKGL
                     PILESAGIAASERFRSVMMTAFTCVIGVIPMLVASGAGAGSRLHVGTTMFFGMSIATV
                     FGIFLIPGLYVLLQTYREKTKERIRHAFSKNEEEV"
BASE COUNT          448 a          674 c          659 g          545 t
ORIGIN      
        1 tcctggcgta tctgttcaag gtaactccgg ctttcgctcg agatctgcga ttccgtggtc
       61 ttcgccgtat ctccgtcgac gggctgcggg aaaccacctg ctgccagggg tgcccatcct
      121 ccgcccagca ccttgaatat gcgcacaatg ttcgagagca tctgtccttc gcttatcgcg
      181 tactgttcct caagagtaag cagggcgctc tgggcgccaa tcacgttgtt gaaatccgtg
      241 agaccgtttt tgtatttgtc ctccgcgatc tcaaacgccg ttcgcgccgc atcgacgcca
      301 cgccggagcg actggttgcg ctcccgttcc tgcgtttccg aagtcagtgc attgcgcact
      361 tccgcaacag cgttcagaac tgtctgctca taagccgcca ggagctgctc ctgtttcgcc
      421 gtctggattt tgatattgct ccgtatcgcg ccgccgtgga atatgggcca cgatatgctg
      481 ggtccgaaac catagcttat tccatcggag agggacaatc cgccagtggt aaaggattca
      541 agacctatcg agccgaacag attgaatttc ggccagagat ccctttgggc cgatttcttg
      601 cgcgcgatct gcgcggccag ccggcgttct gccgaataga tatccgggcg ctggcgaagc
      661 gagttcgccg ggattccgac cagaccgaca gcttcgggtt caggcagagg tctgtattcg
      721 cccagcatct cttccaggct tcccggtacc tggccgacaa gtatcgccag cctgttcatt
      781 gtttcctcaa tgctggtgcg cagcggaggg atcgtcgaac gcgtctgttc catcgtatac
      841 ttcgcctggt tcagtgcaag tccgtccttg agcccggagt cgtgctgcga ctgaagaagc
      901 tccaccgtat ccatctgcag ggccaggttg ctttcggcta cctgaagcct tttctgcaag
      961 gtgcggagcg acgtgtagtt cagggcgacc tcggaggaaa gcgtcaccca tgcgttatga
     1021 agagccccga actcggcctg gaggtcggcc tttccggcct tgatcttttg tgcctgtccg
     1081 ccgaaaatgt cgatctccca cgatgcgtcg accccaaggc tgtagaaatc catgggcccg
     1141 atctggctcc ctgtggagga cccgctcttt ccggtctttg agcgggtaag ggtgtttgaa
     1201 ttgtccagcc agggcagaac cgcggatttg ctgatcccca gcgcggcgcg cgcttccctg
     1261 aattttgccc ttgccgagat gaggtcccgg ttgttttcca gagagtactg tatgagacgg
     1321 gtcaaagttt catccccgag ggcattccac caattcgcaa gtatctcagg agaaggagct
     1381 tccgggacct catcctccgc tgcaagatag gacatcggat atctcttcgc cagggttgtc
     1441 cagtcgccgg tctcaagttc cgtatctttc ggggccggag cattgtccgc cccatacgct
     1501 ccaaaggaga ggagcgccag cagacatagt gacataagta agatcgtcgg ttttttcatg
     1561 atcctacacc tcttcttcgt tcttcgagaa tgcatggcgt atccgctcct tagtcttctc
     1621 cctgtatgtc tgcaggagga catacaggcc cgggatcagg aagatgccga acacggtcgc
     1681 tatgctcatt ccgaagaaca tcgtggtacc gacgtgaaga cggcttcctg cgcctgcgcc
     1741 cgatgcgacg agcatgggaa tgactcctat gacgcaggtg aacgcggtca tcatgacgga
     1801 tctgaaccgt tcggatgcgg cgatacctgc ggattccagt atcgggaggc ccttcacgtc
     1861 atgctgctcc tttgcgaatt cgatgatgag tatggcgttc tttgccgcca gcccgacaag
     1921 cagcagaatt ccaagctggg cgtatatgct gagggatatc cccatgatga atatccccgc
     1981 cagggctccg atcagggcta ccggaagcga gagaatcact cccagcggta ccgtccagct
     2041 ttcgtattga gccacaagga acaggtatcc gaaaaggacg gctatcagca gtatcaggcc
     2101 tgtctgtccg cctgcttccc gttcctggta tgtcatgccc gaccattcga gagagtatcc
     2161 ttcgggaagg gcggaagcga gcttctccat cgccgctatg ccttgccctg tagagtaccc
     2221 gggagccatg aaggctgtga ccgcagcagt gggacagagg ttgtagcggc tgagtgtgcg
     2281 gggcgcaagc gtctttttga gcgtcatgat gctctccagc ggaacg
//
LOCUS       DAOTIL010000413         2324 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminobacteriaceae bacterium isolate AMR_MDS_3026
            NODE_54661_length_2324_cov_3.539004, whole genome shotgun sequence.
ACCESSION   DAOTIL010000413 DAOTIL010000000
VERSION     DAOTIL010000413.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868050
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminobacteriaceae bacterium (wastewater metagenome)
  ORGANISM  Aminobacteriaceae bacterium
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae.
REFERENCE   1  (bases 1 to 2324)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2324)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:19:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,224
            CDSs (total)                      :: 3,198
            Genes (coding)                    :: 3,184
            CDSs (with protein)               :: 3,184
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2324
                     /organism="Aminobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_54661_length_2324_cov_3.539004"
                     /isolate="AMR_MDS_3026"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:3034609"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..997)
                     /locus_tag="P5201_08865"
     CDS             complement(<1..997)
                     /locus_tag="P5201_08865"
                     /inference="COORDINATES: protein motif:HMM:NF024627.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="right-handed parallel beta-helix
                     repeat-containing protein"
                     /protein_id="HRV98688.1"
                     /translation="MNAVNEKTRYTKRGLLLRCILPVFVAAVLLCFAGAARAATFTVT
                     RGDDGGAGTLRNAIGNANPGDTIVFDPSVSVVNVNSYLFVNKPLTITGPVTIKQNVSG
                     QRVLYVTGDCTFNQLTITGGNTGTGSGSQGAGVFSYANLTMNDCTVSGNAGGGGIHNE
                     KTMTLNNCVVRNNTASGFPKAGGISSTDYGYNATTLTMRNCVVENNTCTDGIAGGIYN
                     GVKGTLTMYRCIVRRNSLSSMGGGLYISSSTTTHLADYCQVTENTPDQICCTYGASYT
                     TDGTCTIGSAAGTSAVALSGATEGSSSEPRKTAGEPDVTKVGNDLKNRESAIFKEA"
     gene            1347..1616
                     /locus_tag="P5201_08870"
     CDS             1347..1616
                     /locus_tag="P5201_08870"
                     /inference="COORDINATES: protein motif:HMM:NF014643.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0110001 - toxin-antitoxin complex
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system Phd/YefM family
                     antitoxin"
                     /protein_id="HRV98689.1"
                     /translation="MLAVNYSTYRNKLKEYCDRVTDDNETVIVTRKSEKNVVVMSLDE
                     YNNMMESLRILKNPSYFIELFQSLQQLQQGGIVVKTIEELEAAEQ"
     gene            1613..1876
                     /locus_tag="P5201_08875"
     CDS             1613..1876
                     /locus_tag="P5201_08875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002452576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008219 - cell death [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Txe/YoeB family addiction module toxin"
                     /protein_id="HRV98690.1"
                     /translation="MNIIFSQKAWEEYVAWQNEDKKIVIKINALIKDIARNGMLSGLG
                     QPELLKHALSGLYSRRISLEHRLIYAQDTHGNLLIVKCSGHYE"
     gene            complement(1910..>2324)
                     /locus_tag="P5201_08880"
     CDS             complement(1910..>2324)
                     /locus_tag="P5201_08880"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRV98691.1"
                     /translation="SRTSGRHGSGAVLKDMGVVSGFFFEAPGEPSVYWAGDTVLTDEI
                     RELLRVRMPDVVLTHSCGAVWGEGTLILMDDAQTAEVCRISPESTVVAIHMEAVDHAT
                     VTRKQLRETARKSGLSDERLLIPLDGETVQIRARS"
BASE COUNT          493 a          662 c          632 g          537 t
ORIGIN      
        1 ccgcttcctt gaagatcgcg ctctctctgt tcttcagatc gtttcccacc ttcgtcacat
       61 cgggctcgcc cgccgtcttc cggggttccg acgaacttcc ctccgtggct cccgaaagcg
      121 ccacggcgga ggttccggca gcggagccga ttgtgcaggt gccgtcggtc gtgtaggaag
      181 cgccgtaggt acagcatatc tggtcgggag tgttctccgt cacttggcag tagtcggcga
      241 gatgcgtcgt tgtggaggac gaaatataga gcccccctcc catgctgctc agactgttcc
      301 tcctcacgat gcaccggtac atggtgagcg ttcctttgac gccgttgtag atgccccccg
      361 cgattccatc ggtacacgtg ttgttctcga cgacgcagtt tctcatcgtc agtgtcgtcg
      421 cgttgtatcc gtagtcggtg ctgctgattc ctcccgcctt tggaaagccg gacgcagtgt
      481 tgttcctgac gacgcagttg ttcagcgtca tggttttttc gttgtgaatg ccgcctccgc
      541 ccgcgttccc gctcacggtg caatcgttca tcgtcaggtt cgcgtagctg aacacgcccg
      601 cgccctgcga cccgcttccc gtccccgtgt tgccgccggt gatcgtcagt tggttgaacg
      661 tgcagtcgcc cgtcacgtag agaactctct gcccgcttac gttctgcttg atcgtgacgg
      721 gaccggtgat cgtcagaggc ttgttgacga agagatagct gtttacgttg acgacggata
      781 ccgatggatc gaagacgatc gtgtcgccgg ggttcgcgtt tccgatggca ttccgaagcg
      841 ttcccgcgcc gccgtcgtct cccctcgtta ccgtgaatgt cgccgccctc gccgctcccg
      901 cgaagcagag cagaaccgcc gcaacgaaca ccggcagtat gcaccgcaac aaaagccctc
      961 tcttcgtgta ccgcgttttt tcattcactg cgttcatcgt tttccccctc ttttcatggt
     1021 cgggttcatg ccgctccagc caagcacgat aagaagagcg tccgtcctct tttgggtcgt
     1081 tccgcgagct atcataaacc ttcacacggt gtaaaagtca aggaggagtt ttaaagattc
     1141 gccgttttta aaataaacat tcccggtcaa tccggagtgt aaggttgcct gctgtcgtct
     1201 tgggaggtcc ttcaaacgac gttattgaaa tgtaaaaatt tccacatgac cgactccagt
     1261 tttcagcctg cttgcttttc aactcgaata aacgtacaat aacttgtaca atacttgtaa
     1321 gaaaacccaa agaaggagga gaggacatgc tggcagtgaa ctactccacg tatcggaata
     1381 aattaaaaga gtattgcgac cgggtgacgg acgacaatga aaccgtgatc gtcactcgaa
     1441 agagtgaaaa gaatgtcgtc gtcatgagcc tcgacgagta caacaacatg atggagagct
     1501 tgagaatcct gaagaatccg tcgtacttca tcgaactctt tcagtcattg cagcagcttc
     1561 aacagggtgg aatcgtcgtc aaaaccatcg aggagctgga agcggccgag caatgaatat
     1621 cattttttcg caaaaagcat gggaagagta tgtagcgtgg cagaatgaag ataaaaagat
     1681 cgtcattaaa atcaacgcgc tgatcaagga tattgccaga aacggcatgc tgagcgggtt
     1741 aggacaaccc gagctcctga agcacgctct ttccggcctg tatagcagaa gaatttcact
     1801 ggaacaccgt ttgatatacg ctcaggacac gcacggcaat ctgctgatcg tgaagtgctc
     1861 aggtcactac gaataatccc cattcacgaa agaacatgag ttttcctgat tagctgcggg
     1921 ccctgatttg gacagtctct ccgtcgagcg ggatcaggag gcgctcgtcg gagagaccgc
     1981 ttttccgcgc cgtctcgcgg agctgcttgc gggtcactgt ggcgtggtcc accgcctcca
     2041 tgtggatggc gacgaccgtg ctttccggcg aaatgcggca gacctcggca gtctgcgcgt
     2101 cgtccatcag gatgagcgtt ccctcgcccc agaccgcgcc gcacgagtgc gtcaggacga
     2161 cgtcgggcat acggacgcga agcaactcgc ggatctcgtc ggtgaggacg gtatcccccg
     2221 cccagtagac gctcggttct ccgggcgctt cgaagaagaa gcccgagaca acgcccatgt
     2281 ccttcagcac cgcgcccgaa ccgtggcgcc ccgatgtgcg ggag
//
LOCUS       DAOTIL010000414         2315 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminobacteriaceae bacterium isolate AMR_MDS_3026
            NODE_54930_length_2315_cov_3.726991, whole genome shotgun sequence.
ACCESSION   DAOTIL010000414 DAOTIL010000000
VERSION     DAOTIL010000414.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868050
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminobacteriaceae bacterium (wastewater metagenome)
  ORGANISM  Aminobacteriaceae bacterium
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae.
REFERENCE   1  (bases 1 to 2315)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2315)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:19:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,224
            CDSs (total)                      :: 3,198
            Genes (coding)                    :: 3,184
            CDSs (with protein)               :: 3,184
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2315
                     /organism="Aminobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_54930_length_2315_cov_3.726991"
                     /isolate="AMR_MDS_3026"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:3034609"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1280)
                     /locus_tag="P5201_08885"
     CDS             complement(<1..1280)
                     /locus_tag="P5201_08885"
                     /inference="COORDINATES: protein motif:HMM:NF015894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FapA family protein"
                     /protein_id="HRV98692.1"
                     /translation="MSDMRMANIDKLTLEIEKLLADAEVTGALPQVEDRDASWEVRIS
                     DDGLTAYLDMYPAVGNGVSPDPLGICTELEEMGIRRVDRDRVMALVTLCDSGYPASGE
                     DAVVARGVPPESPSPGRIEFLVLMERAKRSEDDEASVDWKNLWLTPSVHEGDVIAKIY
                     PPKEGTDGVDVYGNPLPAASHTFFRIRYGDGVNVSEGEDGTVEIASARTVGQPVFRDN
                     VLDVAPLLVINGDVNISTGSIDFTGSVLVSGSVLEGFSVKAERDVNVQGWVYNARVQA
                     GGSCSVKGGVTGERSLLLAGEDVRVGVVEYAEISASRKVEVFGYALFAMLEAGESIYV
                     QGRNRRGVVGGTCIAGACIEALSAGSPMESSTQLEAGRDPFQARILQELERKKEALLA
                     MQKKIEQAILSIKGTGPNFDMDTLTEKEKGKLFLL"
     gene            complement(1407..1673)
                     /locus_tag="P5201_08890"
     CDS             complement(1407..1673)
                     /locus_tag="P5201_08890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008709104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004518 - nuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GIY-YIG nuclease family protein"
                     /protein_id="HRV98693.1"
                     /translation="MEAWVYILRCADGTLYTGWTFDTEARLCVHNSGKGARYTASRLP
                     VEMVYRERVASKSDALRRERLIKRMRRSAKERLIAGACSVSLPE"
     gene            complement(1676..2239)
                     /locus_tag="P5201_08895"
     CDS             complement(1676..2239)
                     /locus_tag="P5201_08895"
                     /inference="COORDINATES: protein motif:HMM:NF014689.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="manganese efflux pump MntP family protein"
                     /protein_id="HRV98694.1"
                     /translation="MSFVEMLLSAASLSMDAFAASLGIGACLGAGASAAAFRVGAACG
                     GFQFAMPLAGWWLGSRFLESISGYDHWIAFFLLLIVGGNMIREAFTKDEPNCPTADPS
                     CGFALLFVALATSIDALAVGIGFAAIGAPVMPLSSSAGIITALLSFGGVLAGFRTALF
                     LGRKAEFAGGVVLCIIGANILRMHLAA"
BASE COUNT          509 a          747 c          624 g          435 t
ORIGIN      
        1 aggagaaaaa gcttgccctt ttccttctcg gtaagagtgt ccatatcaaa attcgggcct
       61 gttcctttaa ttgaaagaat cgcttgctct atcttcttct gcatcgcaag gagcgcctct
      121 ttcttccgtt caagctcctg gagtattctg gcctggaacg ggtcgcgtcc cgcctcaagc
      181 tgcgtggacg actccatcgg agagcctgcg gagagcgcct cgatgcaggc gccggcgata
      241 caggttccgc ccacgacgcc cctgcggttc ctcccctgga cgtagatcga ttcgcccgcc
      301 tcaagcatcg caaagagcgc gtagccgaag acttcgacct tccgtgaggc gcttatttcc
      361 gcgtattcaa caacgccgac gcgcacgtct tcgcctgcga gaagcaacga gcgttctccc
      421 gtcacgcccc cttttacact gcacgagccc cccgcctgca ctcgcgcgtt atacacccac
      481 ccctgcacat tgacgtctct ctccgccttg acggaaaaac cttcgagcac cgaaccggag
      541 acaaggacgg agccggtaaa atcaatgctg ccggtggaga tgtttacgtc gccgttgatg
      601 accagcagag gcgccacatc cagcacatta tcccggaaga caggttggcc gaccgtccgc
      661 gccgaagcga tttcaaccgt gccgtcttca ccctccgaga cgttcacacc gtcgccgtaa
      721 cgaatgcgga aaaacgtgtg tgaagcagca ggaagaggat tcccgtagac atccaccccg
      781 tcggttccct ctttcggagg ataaatcttc gcgatgacgt ctccttcgtg cacggagggg
      841 gtgagccaga gatttttcca gtcgacggac gcctcgtcgt cctcggagcg cttcgccctt
      901 tccataagaa ccagaaactc gatccggccc gggctcggcg actccggcgg aacacctctc
      961 gcgacgacag cgtcctcccc tgaggccggg tatccggaat cgcacagagt aaccagcgcc
     1021 atcactctgt cgcggtcgac tcggcgtatt cccatctctt caagctccgt gcagattccg
     1081 agcggatcgg gggaaacgcc gtttcccact gcggggtaca tgtcgagata cgctgtcaag
     1141 ccatcgtcag aaatacgcac ctcccacgac gcatccctgt cctccacctg agggagagct
     1201 cctgttacct ccgcgtcggc gagaagcttc tcgatttcca aggtcagttt gtcgatgttg
     1261 gccattcgca tatcgctcat acggaagacc ccctctcatt ttccacaaca caagagcata
     1321 cccagcttca gaatactctt ttttctccgg aagggcaaga agagggttcg aaccgctggg
     1381 tattttgcga agaagaataa caagggctat tccgggagac tcaccgagca ggcgccggca
     1441 ataagccgct ctttcgcgct gcggcgcatc ctcttaatca gccgctctct gcgcagcgca
     1501 tcgctctttg aggccacgcg ttccctgtac accatttcga ccggaaggcg cgatgcggtg
     1561 taccgcgcgc ccttgccgct gttatggacg cagagccgag cttccgtatc gaaggtccag
     1621 ccggtgtaga gcgtcccgtc ggcgcagcgg aggatgtaga cccacgcctc cacggttacg
     1681 ccgccaggtg catcctcagg atattggcgc cgatgatgca gagcacgacg ccgcccgcga
     1741 actccgcctt gcggccgagg aagagggccg tgcggaagcc tgcgagcacc ccgccaaaag
     1801 agagcagcgc cgtaatgata cccgcggacg aagagagcgg catgacaggg gctccgatgg
     1861 cggcaaaacc gatgcccacg gcgagtgcat cgatggaggt cgccagcgcc acaaaaagga
     1921 gtgcaaaacc gcatgaggga tccgccgtcg gacagttcgg ttcatccttc gtgaaggcct
     1981 cccggatcat gtttcctccg acgatgagca gcaggaaaaa ggcaatccag tgatcatacc
     2041 ccgatatgga ctccaaaaaa cggctcccca gccaccaacc cgcaagcggc atggcgaact
     2101 gaaaccctcc gcacgcagcc ccgacgcgga atgccgcggc cgaggctccg gcgcccaggc
     2161 acgcgccgat acccagggag gcggcaaaag cgtccatgga aagcgacgcg gcggaaagca
     2221 gcatttcaac aaacgacacg aagcatcata cctttcttca ctaaaaaaag accttctcca
     2281 tacgcacaag atgtcgaagg ccggtttttt tattt
//
LOCUS       DAOTIL010000415         2313 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminobacteriaceae bacterium isolate AMR_MDS_3026
            NODE_55015_length_2313_cov_2.438884, whole genome shotgun sequence.
ACCESSION   DAOTIL010000415 DAOTIL010000000
VERSION     DAOTIL010000415.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868050
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminobacteriaceae bacterium (wastewater metagenome)
  ORGANISM  Aminobacteriaceae bacterium
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae.
REFERENCE   1  (bases 1 to 2313)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2313)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:19:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,224
            CDSs (total)                      :: 3,198
            Genes (coding)                    :: 3,184
            CDSs (with protein)               :: 3,184
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2313
                     /organism="Aminobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_55015_length_2313_cov_2.438884"
                     /isolate="AMR_MDS_3026"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:3034609"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..306
                     /locus_tag="P5201_08900"
     CDS             <1..306
                     /locus_tag="P5201_08900"
                     /inference="COORDINATES: protein motif:HMM:NF018513.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter large permease subunit"
                     /protein_id="HRV98695.1"
                     /translation="CFLEGNAIFLITLPIFIPVAKMFQIDLVNFGVVMTLLIMIGNLT
                     PPVGMCLFAVDSFAKVGILPLSKEVVPYLVVILGVTFVIAFVPDIALFLPRLMMGGM"
     gene            309..878
                     /locus_tag="P5201_08905"
     CDS             309..878
                     /locus_tag="P5201_08905"
                     /inference="COORDINATES: protein motif:HMM:NF016200.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0031317 - tripartite ATP-independent
                     periplasmic transporter complex [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015740 - C4-dicarboxylate transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter small permease"
                     /protein_id="HRV98696.1"
                     /translation="MDSFLHKTDIAVQRILRIVVLFFFLALAFLLAYNVLLRLTNDLS
                     TFLTERGFSEAAGAVRGWLPITSMHWFDEIVELCFAALIFYGAASLWASGGHFSVGDW
                     ISPRLPYERLRNLYRVFVDLLGLAFLWVFFFYSLRLTLRATELTTVFQIHKSALYCCM
                     PVSSFIMLLYSLRDVWNDGKRLFSPGRGA"
     gene            1060..1866
                     /locus_tag="P5201_08910"
     CDS             1060..1866
                     /locus_tag="P5201_08910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014163722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit beta/FixA
                     family protein"
                     /protein_id="HRV98697.1"
                     /translation="MKIAVLIKQVPDTDDVRMDPEKGTMIREGVGAIVNPLDLNALQA
                     ALDLKAASAEAAEASSSEVTVITMGPPKAHEALRECIATGADRGVLLSDRAFAGSDTW
                     ATAKVLAAAVEHTGPYDLVLAGEKATDGETGQVGPEVAVLLGIPFSTYVTGLSFTEGK
                     IEVRRMTEEGVQRQRLPFPCLLTVLHALNEPTMPTLAGKKRARRSEIPVLGISDLGLS
                     PEETGLKGSATRVVKIAYPTISRSTEMYDGKRADEGIDRLIEVLKDLAVI"
     gene            1879..>2313
                     /locus_tag="P5201_08915"
     CDS             1879..>2313
                     /locus_tag="P5201_08915"
                     /inference="COORDINATES: protein motif:HMM:NF013200.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit alpha"
                     /protein_id="HRV98698.1"
                     /translation="MTAQKSFFVLGEVRDGRIHSVTRELTGKARELADSVGGRVTTVL
                     LSGGLADSPEDLFRYGADRVLSVEHPSMTLYNQEIETNILAHVLREELPEVVLAGATT
                     SGRTTLPALASKLETGLTADCTGLEMDLETGLLLQTRPAIGGN"
BASE COUNT          418 a          674 c          704 g          517 t
ORIGIN      
        1 tgcttccttg agggaaacgc aatcttcctg atcacgctgc cgatcttcat ccctgtcgcg
       61 aaaatgttcc agatcgacct cgtcaacttc ggcgtggtga tgacgctgct gatcatgatc
      121 ggcaacctga cgccgcctgt tggcatgtgc ctcttcgcgg tggacagttt cgcgaaggtc
      181 gggatacttc ctctttcaaa agaggtcgtc ccgtatctcg tcgtcatact cggcgtgacc
      241 ttcgtcatcg cttttgtccc cgacatcgcg ctcttcctgc cgcgtctgat gatgggagga
      301 atgtaacaat ggactctttc ctccataaaa ccgacattgc cgtccagcgt attctccgaa
      361 tcgtcgttct ttttttcttc cttgcgctgg cttttttgct ggcctacaac gttttgctgc
      421 gcctgacgaa cgatctgtcg acttttctga ccgagcgcgg tttcagcgaa gcggcgggag
      481 ccgtcagagg gtggcttcca attacttcga tgcactggtt cgacgaaata gtggagctct
      541 gtttcgcagc gctcatcttc tacggggcgg catcgctctg ggcgagcgga ggccatttca
      601 gcgtagggga ctggatttcg ccgcgccttc cgtacgagag actccgaaat ctctaccgtg
      661 tgtttgtcga ccttctcgga ctcgcgtttc tgtgggtctt tttcttttac tcgctgcgtc
      721 tgacgcttcg cgccacggag ttgacgacgg tcttccagat tcataagtct gcgctgtact
      781 gctgtatgcc cgtatcgtcc ttcattatgc tcctctattc gctgcgtgac gtctggaacg
      841 acggaaagcg cctcttctct ccgggaaggg gagcctgaaa caggaatatc ctgcgttgtg
      901 tatgcttggg ggcggatgct ctttccgctc cctctttttt gtggaacttg aaaatcagct
      961 gtttttccct cgatggaatc gccgatctcc tgaactttgt ggtatcgtac agcgaggaaa
     1021 ggtgtttcag gtatcattcc actgcaagga ggtttttccg tgaagattgc cgtgctaata
     1081 aaacaggttc ccgatacgga cgacgttcgt atggaccctg aaaagggaac gatgatccgc
     1141 gagggcgtcg gggccatcgt gaacccgctg gatttgaacg ctctccaggc tgcgcttgac
     1201 ctgaaggcgg cctctgcgga ggcggccgaa gcctcttcaa gcgaggtcac cgtgattacg
     1261 atggggccgc ccaaggcgca cgaggcgctc cgcgagtgca tcgcgaccgg agcggaccgg
     1321 ggcgtgctgc tcagcgaccg cgcattcgcc ggaagcgata cgtgggctac ggccaaagtg
     1381 ttggctgcgg ctgtggagca taccggcccc tacgatctcg ttctcgccgg ggagaaggcg
     1441 accgacggcg agacggggca ggttggcccg gaggttgcgg tgctgcttgg catacccttc
     1501 agcacgtacg ttacaggact ctccttcacc gaagggaaga tcgaagtccg acgcatgacg
     1561 gaggagggag ttcaacggca gcgtctgccc ttcccctgtc tgctcacggt gcttcacgca
     1621 ctcaacgagc cgaccatgcc gacgctcgcc ggcaagaagc gggcgcggcg gagcgagatt
     1681 ccggtgctcg gaatctcgga cctcgggctc tcgcccgagg agacggggct caagggctcc
     1741 gcgacccgcg tggtgaagat cgcttatcct actatctccc gttccacgga gatgtacgac
     1801 gggaaacgtg cggacgaggg gatcgaccgc cttatcgagg ttctcaaaga cctcgccgtg
     1861 atttaggagg aacgcgctat gacggcacag aagagctttt tcgtcctcgg agaggtcaga
     1921 gacgggcgca ttcactccgt gacgcgcgaa ctgacgggga aggccaggga actcgccgac
     1981 agcgtcggcg ggcgggtgac gacggtgctc ctctcgggag gactggcgga ttcgcctgaa
     2041 gaccttttcc ggtacggggc cgacagggtc ctgtccgtgg agcacccgtc gatgacgctc
     2101 tacaaccaag agatcgagac gaacatcctc gcccacgtgc ttcgcgagga gctacccgag
     2161 gtggtgctcg ccggtgcgac gacgtccgga cggacgacgc tccccgcatt ggcctcgaaa
     2221 ctggagacgg ggctcaccgc ggactgcacc ggccttgaga tggacctcga aaccgggttg
     2281 ctgctccaga cccgcccggc tatcggcggc aac
//
LOCUS       DAOTIL010000416         2312 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminobacteriaceae bacterium isolate AMR_MDS_3026
            NODE_55050_length_2312_cov_3.031901, whole genome shotgun sequence.
ACCESSION   DAOTIL010000416 DAOTIL010000000
VERSION     DAOTIL010000416.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868050
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminobacteriaceae bacterium (wastewater metagenome)
  ORGANISM  Aminobacteriaceae bacterium
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae.
REFERENCE   1  (bases 1 to 2312)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2312)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:19:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,224
            CDSs (total)                      :: 3,198
            Genes (coding)                    :: 3,184
            CDSs (with protein)               :: 3,184
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2312
                     /organism="Aminobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_55050_length_2312_cov_3.031901"
                     /isolate="AMR_MDS_3026"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:3034609"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1361)
                     /locus_tag="P5201_08920"
     CDS             complement(<1..1361)
                     /locus_tag="P5201_08920"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HRV98699.1"
                     /translation="MTLKIEEHILQSPLIGFAHHRILLDDFGKPVDYEYLAVNATFEK
                     LTGLDAASLLHRTIRQVVPGFEKTGSDWIRIFGEVALGGGEREFEQFSEPLGKWFRVH
                     VYSAERLYFTTLFLDVTESKKQAEELEGFFRVNLDLLCIADIDGNFVKTNAAWGEILG
                     YSSEELDGNRFLDFVHPDDLPATLDAMSALGEGTKVMNFVNRYRCKDGSYRFIEWRSH
                     PKDRLIYAAARDITEHRLIEDELRAATMRAEEMARKAKAASIAKSEFLANMSHEIRTP
                     MNGVLGMTELLLAGALSEEQRQYAQTIRASADSLLVILNDILDISKVEAGKLELKTLD
                     FNLDMLREEFESSMRAVAEHKGLSLFCSVDPDVPRCLRGDPGRLRQIMTNLTGNAVKF
                     THRGEVRTNASVVEQDDRSVLLRFTVRDTGIGIPADKLGTLFEKFTQVDASSTRQFSG
                     TGLG"
     gene            1631..>2312
                     /locus_tag="P5201_08925"
     CDS             1631..>2312
                     /locus_tag="P5201_08925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009200315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:solute symporter family protein"
                     /protein_id="HRV98700.1"
                     /translation="MSMQLFILVAYILVLYGISWYATKLIQQGKGGALQYLLAGRNLP
                     MIVVAAMLAGLAIGGASTVGVAEHAYKAGLSAGWYNAAWGMAGILAGIFAAHYFRRME
                     VNTIPEMMERMYGKSARMISVVAQLLIMMVITSLQYVAGGAVLTALMPDVFTFQTGMM
                     VTAVLFVGITFIGGYWGAGLTNVVNIIVIYGGILSALWNSSEVFGGFESIIAALPEGG
                     PWFDFVSGA"
BASE COUNT          400 a          665 c          733 g          514 t
ORIGIN      
        1 ccgagacccg tgccgctgaa ctggcgggtg gaggaggcgt ccacctgcgt gaacttctcg
       61 aagagcgttc caagtttgtc ggccggaatc ccgatgccgg tgtcgcgcac cgtgaagcgg
      121 aggaggacgg agcggtcgtc ctgttcgact acggaggcgt tggttcgtac ctctccgcga
      181 tgcgtgaact tcacggcgtt ccccgtcagg ttcgtcatga tctggcgcaa tcggcccggg
      241 tcgcccctca ggcagcgcgg aacgtcgggg tcgacgctgc agaagaggga gagccctttg
      301 tgttccgcga ccgcgcgcat ggaggactcg aattcttccc tgagcatgtc gaggttgaag
      361 tccagcgttt tgagctccag cttgccggcc tccaccttcg agatgtcgag gatgtcgttg
      421 aggatgacca gcagggagtc ggcgcttgct ctgatcgtct gggcgtactg ccgctgttcc
      481 tcgctgagag cgccggcaag cagcagctcc gtcattccga gcacgccgtt catgggcgtg
      541 cggatttcat ggctcatgtt ggctaaaaac tcgctcttgg cgatgctggc cgccttggcc
      601 ttccgcgcca tctcctccgc ccgcattgtc gccgcgcgaa gctcgtcctc tatgagcctg
      661 tgttcggtga tgtcgcgcgc agcggcgtag atcagtctgt ctttggggtg agaacgccat
      721 tcgatgaagc ggtacgagcc gtccttgcag cggtagcggt tgacgaagtt catcactttc
      781 gtaccttcgc cgagcgcgct catggcgtcc agcgtcgccg ggaggtcgtc cggatggacg
      841 aagtcgagaa atctgttccc gtccagctct tcggatgagt accccagtat ctccccccac
      901 gcggcgttgg tcttcacgaa gttgccgtcg atatcggcga tgcagagcag gtcgagattc
      961 actcgaaaga agccctccag ctcctccgcc tgtttcttgc tctcggtgac gtcgaggaag
     1021 agcgtcgtga agtagagccg ctcggcggag tagacgtgga cacggaacca cttgccgagg
     1081 ggctcggaga actgctcgaa ctcgcgttcg ccgccgccga gggcgacctc gccgaaaatg
     1141 cggatccagt cggacccggt tttctcgaac cccggaacga cctgacgtat cgtgcggtgc
     1201 agcagactgg ccgcgtcgag tccggtcagc ttctcgaagg tggcgttcac cgcgagatac
     1261 tcgtagtcca ccggcttccc gaagtcgtcg agcaggatgc ggtgatgggc gaagccgatc
     1321 agggggctct gcagtatatg ttcttctatt ttcaacgtca tgctggaagc gcctccgttg
     1381 tgtgtgtgaa tcagcagcac cgggactgtc cgggcgctgc accgttcttt atcatgatta
     1441 ctttgtcgat ttatactcga tacaatcata tcataccgct tctccgggcg ggcttttcaa
     1501 cgccaaccaa ccctgcgaaa gacgtacgag cgccgcagca agtttccacg agacatcagt
     1561 gaacctcttt tcatattgcc gcaaaatgct atactactgg gaaatttctt tttccgaagg
     1621 aggaaggtcg atgtcgatgc agcttttcat tctggtggcg tacattctgg ttttatacgg
     1681 tatttcatgg tatgcgacga agctgatcca gcaggggaag gggggcgccc ttcagtatct
     1741 tctcgccggg cggaatctgc cgatgatcgt tgtggcggcg atgctcgcgg ggcttgccat
     1801 cgggggcgca tcgacggtcg gcgtggccga acatgcgtac aaagcggggc tctcggcggg
     1861 gtggtataac gcggcgtggg gaatggcggg tatcctcgcg ggcatcttcg cggcgcacta
     1921 cttccgccgc atggaagtga atacgattcc cgaaatgatg gagcggatgt acgggaaaag
     1981 cgcccggatg atcagcgtgg tggcgcagct cctcatcatg atggtcatca cctcgctgca
     2041 gtatgtggcg ggcggcgcgg tgctcacggc cctgatgccc gacgtcttca ccttccagac
     2101 aggaatgatg gtcacggcgg tcctctttgt gggcatcacc ttcatcggcg gctactgggg
     2161 cgccgggctg accaatgtgg tgaacatcat agtgatctac ggcggtatcc tctccgcgct
     2221 gtggaactcc tccgaggtct tcggcggctt cgagtcgatc atcgccgcgc tacccgaggg
     2281 ggggccgtgg ttcgacttcg tctccggcgc tg
//
LOCUS       DAOTIL010000417         2311 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminobacteriaceae bacterium isolate AMR_MDS_3026
            NODE_55081_length_2311_cov_3.648050, whole genome shotgun sequence.
ACCESSION   DAOTIL010000417 DAOTIL010000000
VERSION     DAOTIL010000417.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868050
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminobacteriaceae bacterium (wastewater metagenome)
  ORGANISM  Aminobacteriaceae bacterium
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae.
REFERENCE   1  (bases 1 to 2311)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2311)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:19:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,224
            CDSs (total)                      :: 3,198
            Genes (coding)                    :: 3,184
            CDSs (with protein)               :: 3,184
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2311
                     /organism="Aminobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_55081_length_2311_cov_3.648050"
                     /isolate="AMR_MDS_3026"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:3034609"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(85..1056)
                     /locus_tag="P5201_08930"
     CDS             complement(85..1056)
                     /locus_tag="P5201_08930"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRV98701.1"
                     /translation="MSRILYSFSVIAFGLLLGYGVRLASERGLVRLRWSMPVLRTFLQ
                     RLALLGLGPFTFIGALWVVEIREPRLALLAGIGAFCHIFGGLAGALLARLFRLERPQA
                     GAMFCCGFFTNIGAIGGLVTFVFLGEAGYAYVPLFKLFEDMVYYGVGFPVARLYSDTK
                     TTGGVSLAARLTRDPFLLVSIFSLTTGTTLNLLNVPRPEVFSQLNALLIPLTTVILLA
                     SIGMAMRFGKTSAYLKEGVAILGVKFLLVPAVCTSLAALLGLGGIAGGLPLKVVLVLS
                     SMPVAFNALVPPSLYALDLDMANSCWLISVLGMAGLLPFLHVLLTLL"
     gene            1240..1980
                     /locus_tag="P5201_08935"
     CDS             1240..1980
                     /locus_tag="P5201_08935"
                     /inference="COORDINATES: protein motif:HMM:NF019342.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyl-gamma-aminobutyrate hydrolase
                     family protein"
                     /protein_id="HRV98702.1"
                     /translation="MGRPVIGIVGNLLIEQAGFSPGMERSYVNNDYVAAVERAGGIPF
                     ILPVVRGQDVAERQTECVDALLISGGYDMDPLLYGEEPIRALEYVYRETDIFQLRTIK
                     TALKRGQPILAICKGIQLLNVACGGTLYQDLGEIPGSFVKHSQNRKRSCPSHTVIAEG
                     ESLIAELFGGAFPVNSYHHQALKEIGRGLVVTSRAMDGVVESVETEDDSFVVGVQWHP
                     EMMLSGGDEMLPLFLRFAEAAERFALSR"
     gene            1977..>2311
                     /locus_tag="P5201_08940"
     CDS             1977..>2311
                     /locus_tag="P5201_08940"
                     /inference="COORDINATES: protein motif:HMM:NF015962.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0050532 - 2-phosphosulfolactate
                     phosphatase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-phosphosulfolactate phosphatase"
                     /protein_id="HRV98703.1"
                     /translation="MSIRCDVVLSPSETLPEADCWLVIDILRATTTMCAYFDGGGKRL
                     YPAESIEEAFTLRDRLAREGNAPLLMGERNSLPPPGFDLGNSPLELLAAAPATRPEAV
                     MATTNGTRAL"
BASE COUNT          478 a          725 c          742 g          366 t
ORIGIN      
        1 cagttcggcc tttatgcgcg caagagtgaa caagcgtttc gtcctctgga agaatgggaa
       61 tccgccgtgc gggaagcgag gctcttacaa gagcgtcaac agcacgtgaa ggaacggcag
      121 cagccccgcc atgccgagca cggagatcag ccagcagctg ttcgccatgt ccaggtcgag
      181 cgcgtagagc gacgggggca ccagcgcgtt gaacgccacc ggcatggagg agagaacgag
      241 cacgaccttg agcggaagcc ctccggcgat gccgccaagc cccagcaagg cggcgagcga
      301 cgtgcagacg gccggaacca gcagaaactt cacgccgaga atggcgacgc cttctttcag
      361 gtaggcggag gtcttgccga agcgcatggc catgccgatg gaggccagca ggatcaccgt
      421 agtcagcgga atgagcagcg cgttgagctg cgagaagacc tcggggcgcg gcacattgag
      481 cagattcagc gttgttcccg tcgtcagact gaagatggag acgagaagga acggatcgcg
      541 cgtcagccgt gcagcgaggc tcacgccgcc cgtcgtcttc gtatcgctgt agagcctggc
      601 tacgggaaag ccgacgccgt agtagaccat gtcctcgaac agcttgaaga gcgggacgta
      661 ggcgtacccc gcctcgccga gaaagacgaa ggtcaccagt ccgccgatgg ccccgatatt
      721 ggtgaagaaa ccgcagcaga acatcgctcc cgcctgcggt cgttccagcc ggaagagacg
      781 cgccagcagc gcgcccgcaa ggccgccgaa gatgtggcag aaagcgccta ttccggcgag
      841 cagggcaagg cgaggctcgc gtatctccac cacccagagc gccccgatga aggtgaacgg
      901 acccaggccc agaagggcga gccgctgcag aaaggttcgg agcacgggca tggaccatcg
      961 cagccggacg agcccccgct cgctcgcgag ccggactccg taccccagca gcagcccgaa
     1021 ggcgatgacg gaaaaggaat aaagtatccg gctcatactc cggcttcgtt ctttttttcg
     1081 atattataga gcgacttcac aaagaactcc tccttgattc gcatgaaggg tattattccc
     1141 cgtgaagctc ccgatgtcaa cgtgaaaaaa cgcgcccggc gcgctatact tgaaccacac
     1201 acatgcggcg cagaagccgc gcaccaggag gggaaggcaa tgggtcgtcc ggtcatcggc
     1261 attgtgggaa atctgctcat cgagcaagct gggttttcgc ccggcatgga acggagttat
     1321 gtgaacaacg actatgtggc tgcggtggag cgcgcgggag gcatcccgtt cattctgccc
     1381 gtcgttcgcg gacaggacgt ggccgaacgc cagacggagt gcgtcgacgc gctgctcatc
     1441 tccggcgggt acgacatgga tccgctgctg tacggggagg aaccgatacg cgcccttgag
     1501 tacgtctaca gggagaccga catcttccag cttcgcacca tcaagacggc gctgaaacgg
     1561 gggcagccca tcctcgccat ctgcaaggga atccagctgc tgaacgtcgc ctgcggaggg
     1621 acgctctacc aggatctcgg cgagatcccc ggctccttcg tcaaacactc gcagaacaga
     1681 aaacgctcct gccccagcca caccgtgatc gctgaggggg agtcgctcat cgccgaactc
     1741 ttcgggggcg cctttcctgt gaacagctac caccaccagg cgctcaagga gatagggcgc
     1801 ggactcgtcg ttacctcgcg cgcgatggac ggcgtcgtcg agtcggtgga gactgaggac
     1861 gactctttcg ttgtcggcgt gcagtggcac cccgagatga tgctctccgg cggcgacgaa
     1921 atgctgccgc tcttcctccg tttcgcggag gcggcggaac gcttcgcgct ctcgcgatga
     1981 gcatacgctg cgacgtcgtc ctctcgccct cggagacgct cccggaggcc gactgctggc
     2041 tcgttatcga cattctgagg gccaccacga cgatgtgcgc ctacttcgac ggcggcggca
     2101 agcgcctcta tccggcggag tccatcgagg aggcctttac gctgcgcgac aggctggcac
     2161 gggaggggaa cgccccgctc ctgatgggcg agcggaactc gcttcctccc cccggcttcg
     2221 acctcgggaa ctcccctctg gagctgcttg ccgccgcgcc tgcgacgcgc ccggaagccg
     2281 tgatggcgac gaccaacggc acgagggcgc t
//
LOCUS       DAOTIL010000418         2306 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminobacteriaceae bacterium isolate AMR_MDS_3026
            NODE_55252_length_2306_cov_2.823634, whole genome shotgun sequence.
ACCESSION   DAOTIL010000418 DAOTIL010000000
VERSION     DAOTIL010000418.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868050
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminobacteriaceae bacterium (wastewater metagenome)
  ORGANISM  Aminobacteriaceae bacterium
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae.
REFERENCE   1  (bases 1 to 2306)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2306)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:19:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,224
            CDSs (total)                      :: 3,198
            Genes (coding)                    :: 3,184
            CDSs (with protein)               :: 3,184
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2306
                     /organism="Aminobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_55252_length_2306_cov_2.823634"
                     /isolate="AMR_MDS_3026"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:3034609"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..296)
                     /locus_tag="P5201_08945"
     CDS             complement(<1..296)
                     /locus_tag="P5201_08945"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRV98704.1"
                     /translation="MSQGKFQMQKMLTRYGTILALIVLCAGFSFAVPGFLAGENGMNI
                     LRQIALLAVISEGFTMCLIVGELDLAFPHIASLSGALVAGLIFGGMAPLPAVLLS"
     gene            complement(283..1779)
                     /locus_tag="P5201_08950"
     CDS             complement(283..1779)
                     /locus_tag="P5201_08950"
                     /EC_number="7.5.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter ATP-binding protein"
                     /protein_id="HRV98705.1"
                     /translation="MSILETRNLTKEFPGVRALSDFSFTLEKGEIHGLVGENGAGKST
                     LVKILAGVYTPTSGSFLLGGREMRFRSPRDAAERVGVVHQERELIPHFSGWENLFLGM
                     EFSSLGFLRKRRMREEALRFVEKYSMNIDLDLPASELGSGRQEMLAILKVLFRDPEVV
                     IFDEPTAPLSVKECESLFALIRDLKAKGLPILYISHHLSEVLHLADRITVLRNGRKVS
                     TVHAKDADEQTLIRLMIERDMESQYPKRSVSAGEDVFTMERASSRQGRVRDVSIHVRR
                     GEIVGFAGLVGAGRTELAKAVFCGSRFEEGRMTLAGKPFSSRGPGESIRKGIAMIPEK
                     RREEGVVADMTVGENLVLPLLKQLASFGFLRSRQCDQHANSVVDKLAIKISGLSQETR
                     TLSGGNQQKVSVGKWFGTPSELWIFDEPTQGIDVDAKREVYTIMGDLAERGAGVWFIS
                     SDLRELLAIADRIYVMKDFRIAGEFLPPFDDEEILGSMMGEGLRNVAG"
     gene            complement(1776..>2306)
                     /locus_tag="P5201_08955"
     CDS             complement(1776..>2306)
                     /locus_tag="P5201_08955"
                     /inference="COORDINATES: protein motif:HMM:NF015402.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BtpA/SgcQ family protein"
                     /protein_id="HRV98706.1"
                     /translation="FGLSVLADPEAAVAIGAAVEADFVRIFLSWVFVGDWGIVDPDAG
                     KLQRLRASLAPSMKVFANISGHTEPLGGRKLEDIARGAVKFGLADAVCLAGTTAGSEI
                     PEEDLRGARRGAEGYPVIAGTGVCVENVERMLSLADGVIMGTSVKVDRDTFKPVDPLK
                     AAEFIRCAKDARDKMQ"
BASE COUNT          463 a          784 c          623 g          436 t
ORIGIN      
        1 gagagcagca ccgcgggaag cggcgccatt cccccgaaga tcagccccgc caccagcgcg
       61 ccggaaaggc tggcgatgtg cggaaaggcc aggtccagct ctcccacgat gaggcacatc
      121 gtgaatcctt ccgagatcac cgccaaaagg gcgatctgcc tgagaatatt cattccgttc
      181 tcacccgcca aaaaccccgg caccgcaaaa gaaaaccctg cgcacaagac gatcagggcg
      241 agaatcgtcc cgtagcgcgt cagcattttt tgcatttgaa atttaccctg cgacatttcg
      301 gagcccctcc cccatcatgc ttccaagaat ttcttcatcg tcgaacggag ggaggaactc
      361 ccccgcaata cgaaaatcct tcatgacgta tatccggtcg gcgatggcga gcagctcgcg
      421 aagatccgag ctgatgaacc acacgcccgc ccctctttcc gcgagatcgc ccatgatggt
      481 gtagacctcg cgttttgcgt ccacgtcgat cccctgcgtc ggctcgtcga aaatccagag
      541 ttccgaaggg gtcccgaacc attttccaac ggagaccttc tgctggtttc ctccgctgag
      601 cgtcctcgtc tcttgcgaga gaccggagat ctttatggcg agcttgtcca cgacgctgtt
      661 cgcgtgctga tcgcactgcc tcgatcgaag aaagccaaac gacgcgagct gcttcagaag
      721 agggagcacg aggttctccc ccaccgtcat atcggcgacg acgccctctt cgcgcctctt
      781 ctccgggatc atcgcaatcc ccttccgtat gctctcgccc ggaccgcggg aggaaaaggg
      841 tttccccgcc aacgtcatcc gcccctcctc gaagcgagag ccgcaaaaga ccgctttcgc
      901 caattccgtg cggcctgcgc cgacaagccc ggcgaacccg acgatctcgc ctctgcgaac
      961 atgtatcgaa acgtcgcgca ctcttccctg ccgggaggaa gcgcgttcca tcgtgaacac
     1021 gtcctccccc gcggaaacgc tccgtttggg gtactggctc tccatatccc gctcgatcat
     1081 caaacggatc agcgtctgtt cgtccgcatc cttcgcgtgc accgtggaaa cctttctccc
     1141 gttccggaga accgtgatcc ggtcggcgag gtgcaagacc tctgaaaggt ggtgggagat
     1201 atacaagatg gggagccctt tcgccttcag gtccctgatc agagcgaaga gactctcgca
     1261 ctccttcacg ctcagcggcg ccgtgggttc gtcgaaaatg acgacctccg gatcacggaa
     1321 gagcaccttg aggatggcga gcatctcctg gcggccgctg ccaagctccg aagcgggaag
     1381 atcgaggtcg atgttcatcg aatacttctc gacgaaacga agcgcctctt cgcgcattcg
     1441 tcttttccgc agaaaaccga gagaggagaa ctccatcccg agaaagagat tctcccatcc
     1501 ggaaaagtgc gggatcagtt cccgctcctg atggacaacg cccacccttt ccgcggcgtc
     1561 gcgcggcgac cggaaacgca tctctctccc gccgagcagg aacgacccgg aagtcggcgt
     1621 atacacgccg gcgagtatct tcaccagggt ggacttcccg gcgccgttct ctcccacaag
     1681 accatgaatc tcccccttct ccagcgtgaa ggagaaatcg gaaagcgccc ggactccggg
     1741 gaattccttg gtgaggttcc gggtctcgag aatactcatt gcatcttatc cctggcgtcc
     1801 ttcgcgcagc gtatgaactc cgccgctttc agcgggtcga ccggcttgaa ggtgtccctg
     1861 tccaccttga cgctcgtccc catgatgacg ccgtcggcaa gggagagcat tctttcgacg
     1921 ttctccacgc atactcccgt cccggcgatc acgggatacc cttccgctcc gcgcctggcg
     1981 ccgcgcagat cctcctcggg aatttcgctc cccgccgtgg ttcccgcgag gcagaccgcg
     2041 tcggcgagtc cgaacttgac cgctcctctc gcgatatcct ccagtttccg ccctcccaga
     2101 ggctccgtat gccccgagat gttggcgaac actttcatcg agggagcaag cgacgcccgc
     2161 aggcgctgga gctttcccgc gtcggggtcc acgatccccc agtcgcccac gaagacccat
     2221 gagaggaaga tgcggacgaa atcggcctcc acggccgcgc cgatcgccac ggccgcctcc
     2281 ggatcggcca gtacggagag gccgaa
//
LOCUS       DAOTIL010000419         2301 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminobacteriaceae bacterium isolate AMR_MDS_3026
            NODE_55402_length_2301_cov_4.163847, whole genome shotgun sequence.
ACCESSION   DAOTIL010000419 DAOTIL010000000
VERSION     DAOTIL010000419.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868050
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminobacteriaceae bacterium (wastewater metagenome)
  ORGANISM  Aminobacteriaceae bacterium
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae.
REFERENCE   1  (bases 1 to 2301)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2301)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:19:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,224
            CDSs (total)                      :: 3,198
            Genes (coding)                    :: 3,184
            CDSs (with protein)               :: 3,184
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2301
                     /organism="Aminobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_55402_length_2301_cov_4.163847"
                     /isolate="AMR_MDS_3026"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:3034609"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1957
                     /locus_tag="P5201_08960"
     CDS             <1..1957
                     /locus_tag="P5201_08960"
                     /inference="COORDINATES: protein motif:HMM:NF012905.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="HAD-IC family P-type ATPase"
                     /protein_id="HRV98707.1"
                     /translation="LVVVGLAALMMLAGRTLHDFAWGDLFFAAIGFAVAAIPEGLPAI
                     LTITLALGVQRMARRNAITRKLNAVETLGSVTVICSDKTGTLTRNEMTVRYAITPEGE
                     YEVSGTGYRPEGEISLDGSAVSAEERDDLNALVETMAVCNDSEIVEEDGEWRVTGEPT
                     EGALRTLARKASFDPARYERIASIPFESENKFMATLERLPGGGVRILFKGAPDRLLER
                     CGQQRGADGTVQPLDRESWEQRMEELGGEGLRVLAAAFKDADEGKSGLDMEDVENDMV
                     FLGLVGIIDPPRPEAIEAIKICRDAGIKVKMITGDHAGTASAIGREMGITGTGLAVTG
                     EELEKASDEELRTIVRDADIFARTSPEHKLRLVQALQANGEVVAMTGDGVNDAPALKR
                     ADVGVAMGIKGTEATKEAADIVLADDNFISIERAVEEGRTIYDNLRKAILFILPTNGA
                     EALVILAAVVFGLSLPLTPVQILWVNMVTAVTLALALAFEPAEPGVMQRPPRSPDAPI
                     LGGLFLWRIAFVSVLIGGATIAVFLVERRLGMPLETARTLAVNTLVCGQAFYLFNSRY
                     LRESSIAPDRVFANRAAWIAVGVLAVLQIVFVYAPFMQLWFGSAPLELRHWLIPLSLG
                     AAVFIVVEAEKNVTRRVRAANGRRSGV"
     gene            2004..>2301
                     /locus_tag="P5201_08965"
     CDS             2004..>2301
                     /locus_tag="P5201_08965"
                     /inference="COORDINATES: protein motif:HMM:NF014418.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0005992 - trehalose biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trehalose-phosphatase"
                     /protein_id="HRV98708.1"
                     /translation="MKKKKIDSPAGAGFNAPPGNGERCAAEEILQTGDPLPSALDGFD
                     AIARKAEGKRIAIFLDYDGTLSPIVETPEQAIMAADMREAVVKLAECTPLGIISG"
BASE COUNT          421 a          681 c          800 g          399 t
ORIGIN      
        1 actcgtcgtc gtcggtttgg cggcgctgat gatgctggcc ggccggacgc ttcacgactt
       61 tgcatggggg gatctcttct tcgcggccat cggatttgcc gtcgcggcca tccccgaagg
      121 gctgcccgcc attctgacga ttacgctcgc gctcggcgtt cagcggatgg cccgccggaa
      181 cgcgatcacc agaaagctga acgccgtcga gacgctcggc tccgtgacgg tgatctgctc
      241 ggacaagaca ggcacgctca cccggaacga gatgacggtg cgctacgcga ttacacccga
      301 gggggaatac gaagtttcgg ggacgggata ccgtcccgag ggggagattt cgctcgacgg
      361 gagcgcggtc tccgccgaag aacgcgatga cctcaacgcc ctggtggaga cgatggctgt
      421 ctgcaacgac tcggagatcg tcgaggagga cggagagtgg cgagtgaccg gcgagcccac
      481 ggaaggggcg ctgcggacct tggcgcgcaa ggcgtcgttc gatccggccc ggtacgagcg
      541 gatcgcatcc attcccttcg agtcggagaa caagttcatg gcgacgctcg aacggcttcc
      601 cggaggaggc gtcaggatcc tgttcaaggg cgccccggat aggctgctgg aaaggtgcgg
      661 acagcagagg ggcgcggacg gaactgttca gccgctggac cgggagtcgt gggaacagcg
      721 gatggaggag ctgggcggcg aagggcttcg cgtgcttgcc gccgccttca aagatgcgga
      781 cgagggaaag agcgggctgg atatggagga tgtcgaaaac gacatggtgt tccttggcct
      841 cgtcggcatt atcgacccgc ctcgtccgga ggcgatagag gccatcaaga tatgcaggga
      901 cgcgggaatc aaggtgaaga tgatcaccgg cgaccatgcg gggaccgcaa gcgccatcgg
      961 acgcgagatg ggcattaccg gtacgggcct tgccgttacc ggcgaggaac tggagaaggc
     1021 ttccgacgaa gagctgcgca cgatcgtacg ggacgcggac atctttgccc gcaccagccc
     1081 ggagcacaag ctgcggcttg tgcaggcgct tcaggcgaac ggcgaagttg tcgcgatgac
     1141 cggcgacggc gtgaacgacg ctcctgcgct caagcgggcc gacgtcggcg tggccatggg
     1201 gatcaaaggg acggaggcga ccaaggaggc ggcggacatc gtgctggcgg acgacaactt
     1261 catctccatc gagcgcgcgg tggaggaggg gcgcaccatc tacgacaatc tgcggaaggc
     1321 cattctcttc atcctgccga cgaacggcgc cgaggcgctg gtcattcttg cagccgtggt
     1381 cttcgggctg tcccttccgc tgacgcccgt gcagatcctc tgggtcaaca tggtgaccgc
     1441 cgtcacgctc gccctcgctc ttgccttcga gccggccgag ccgggagtga tgcagcgccc
     1501 tccgcgctcg cccgacgccc cgattctcgg cggactcttc ctgtggcgca tcgcgttcgt
     1561 ctccgtgctg atcggggggg cgacaatcgc cgtcttcctt gtggaacgcc ggctcggaat
     1621 gccgctggag acggcgcgga cgctcgcggt caatacgctc gtctgcggac aggcgttcta
     1681 tcttttcaac agccggtatc tccgggagtc gagtattgcg cccgacaggg ttttcgcaaa
     1741 ccgcgccgcg tggatagcgg taggcgtgct ggccgtactc cagatcgtct tcgtctacgc
     1801 tccgtttatg cagctctggt tcggctcggc gcctctggag cttcgccact ggctgattcc
     1861 cctctctctg ggcgcggcgg tcttcatcgt cgtcgaagcg gaaaagaacg tgacgcgccg
     1921 tgtgcgcgcc gcgaacggac gccggagcgg cgtgtagtcc gaacaggcgt tggaagagat
     1981 atcgaagaag cggaggtata gaaatgaaaa aaaagaaaat cgactccccg gcgggggctg
     2041 gtttcaacgc gccaccgggg aacggagaga ggtgcgcggc cgaggagatc cttcaaaccg
     2101 gcgatccgct tccctccgct ctggacggtt tcgacgcgat cgcccgcaag gcggagggaa
     2161 agcggattgc gatcttcctt gattacgacg gcacgctctc tccgatcgtg gagactccgg
     2221 agcaggcgat tatggccgcg gatatgcggg aggcggtcgt caagctcgcc gaatgtactc
     2281 cgctcgggat catcagcggc c
//
LOCUS       DAOTIL010000420         2300 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminobacteriaceae bacterium isolate AMR_MDS_3026
            NODE_55456_length_2300_cov_2.886860, whole genome shotgun sequence.
ACCESSION   DAOTIL010000420 DAOTIL010000000
VERSION     DAOTIL010000420.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868050
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminobacteriaceae bacterium (wastewater metagenome)
  ORGANISM  Aminobacteriaceae bacterium
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae.
REFERENCE   1  (bases 1 to 2300)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2300)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:19:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,224
            CDSs (total)                      :: 3,198
            Genes (coding)                    :: 3,184
            CDSs (with protein)               :: 3,184
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2300
                     /organism="Aminobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_55456_length_2300_cov_2.886860"
                     /isolate="AMR_MDS_3026"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:3034609"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..809)
                     /locus_tag="P5201_08970"
     CDS             complement(<1..809)
                     /locus_tag="P5201_08970"
                     /inference="COORDINATES: protein motif:HMM:TIGR02452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR02452 family protein"
                     /protein_id="HRV98709.1"
                     /translation="MENKTEKLILLPCFDSPEAAQACRRDLKVPREKAIALGTSAVEA
                     CDRGYYFDENGAKVDWGQLVMGACFDARSIPPDFSLQTPSRAPFPETTVQVRNETTLS
                     ACFRFLEQGVRPLALNFANGLHPGGGFLHGALAQEETLCRSSALFMTIRNDPMYAAHA
                     ERTLPDSTDWVILSRGVPVFRRDDGSALGKPVLVDFITCAAPYAPGVGQPESGDLLRR
                     RISRVLRIAFVFGHPTLVLGAWGCGAFGNDSQRTAKDFREALETEFAGAFS"
     gene            complement(940..1857)
                     /locus_tag="P5201_08975"
     CDS             complement(940..1857)
                     /locus_tag="P5201_08975"
                     /inference="COORDINATES: protein motif:HMM:NF024109.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HRV98710.1"
                     /translation="MKKRLPEALRATSAIFLVLLLLLFTTGQPLHAVASEESGTYADF
                     DGLRVHYVDEGPRERPALVFIHGWSCDVSFWRFQTEELRDKFRVIAIDLPGFGLSDKP
                     ADRAYTLDLFAGAARAVVEAAGVEKPVLIGHSMGYPVTTKYLALYPGEARAIVNVDGA
                     YFRSPSNPRTIKELKEMNAFLEGLLGPERPQFIGFFIESTFYGRTPQPLRTEIMEKMA
                     SADEYAANSSMKEFIVPENWEERSYDVPALAVYSAHEYVPPDHEAYLRTVFPNLTYEE
                     WPDTGHFLMMEAPERFNAALVKFLEGLPE"
     gene            complement(1873..2205)
                     /locus_tag="P5201_08980"
     CDS             complement(1873..2205)
                     /locus_tag="P5201_08980"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRV98711.1"
                     /translation="MNVNSVGTTRPLPPLPVEDIVEEKVEETKEEGLKKVEKAKRAVE
                     TERLEIMDRHHETVKKSAETYDKIVKKRLMEKEIEERRLQQRELLAERNEEAERRRDE
                     MRKAAEKD"
BASE COUNT          457 a          742 c          636 g          465 t
ORIGIN      
        1 tgtgaaaacg ctccggcgaa ctcggtttcc aacgcttcac ggaagtcctt cgctgtccgc
       61 tgcgaatcgt tgccgaaggc gccgcacccc cacgcgccca aaacgagcgt cggatgcccg
      121 aagacaaagg caatccggag cacacgcgaa atccgacggc gcagcaaatc ccccgattcc
      181 ggctgcccga cgccgggcgc atacggcgcg gcgcacgtga tgaagtccac cagaactggt
      241 ttcccgagcg cgctgccgtc gtctctgcga aatacgggaa cacctcggga aagaatcacc
      301 cagtccgtgg agtcggggag ggtccgttcg gcatgcgcag cgtacatcgg gtcgtttctt
      361 atcgtcatga aaagggcgct cgaacggcag agcgtctcct cctgcgcgag cgcgccgtga
      421 aggaagccgc ctccgggatg aagtccgttg gcgaagttca acgccagcgg acgaacgccc
      481 tgttcgagaa atcgaaagca ggcggacagg gtcgtttcgt tccgcacctg gacggtcgtc
      541 tcgggaaaag gcgcgcggga gggcgtctgc agcgaaaaat ccggagggat gctccgtgcg
      601 tcgaagcacg cccccatgac gagctgcccc cagtccacct tcgccccgtt ctcgtcgaaa
      661 tagtaccccc tgtcgcacgc ctccaccgca gaagtgccaa gggcgatcgc cttctcgcgg
      721 ggaaccttca gatcccggcg gcacgcctgc gccgcttccg gggagtcgaa acagggcagc
      781 agaatcagtt tttcggtttt gttttccata gcatccctcg cagatcaact ttcatggaac
      841 cacaaacaga acggctaatg aacttcacat cctaaaacca aacgcatatc caggggaatg
      901 cgaagaagga ggagggaaaa tctcccctcc tccttcactc tactcgggca gcccttccag
      961 gaacttcacc agcgcggcgt tgaagcgctc aggggcctcc atcatcagga agtggcccgt
     1021 atccggccac tcttcgtagg tcagattcgg aaagaccgtc cgcaggtatg cctcgtgatc
     1081 gggcgggacg tattcgtgcg cggagtagac cgccagggcg ggaacgtcgt aggagcgctc
     1141 ttcccagttc tccggtacga tgaactcttt catcgagctg ttcgccgcat attcatcggc
     1201 ggatgccatc ttctccatta tctcggtacg caacggctgc ggagttctgc cgtagaacgt
     1261 ggattcgatg aagaagccga tgaattgcgg acgctcgggg ccaagcagcc cctcaaggaa
     1321 cgcgttcatc tctttcagtt ccttgatcgt cctcgggttc gacggagagc ggaagtaggc
     1381 gccgtccacg ttgacgatcg cccgggcctc ccccggatag agcgcgaggt acttcgtcgt
     1441 cacggggtac cccatgctgt gccctatcag gacgggtttc tcaactccgg ccgcctcgac
     1501 gaccgcccgg gccgcgcccg caaagagatc gagcgtgtac gccctgtcgg cgggcttgtc
     1561 gctcagcccg aatcccggca ggtcgatcgc gatcacgcga aacttgtcgc gcagctcctc
     1621 ggtctgaaac cgccagaagg agacgtcgca cgaccaaccg tgaatgaaga cgagcgcggg
     1681 gcgctccctg ggcccttcat ccacataatg caccctgagt ccgtcgaaat cggcgtacgt
     1741 accgctctct tcactggcca ccgcatgcaa cggctgtcct gttgtaaaaa gcaaaagcaa
     1801 caacacaaga aaaattgccg aagtcgctct cagcgcttcg ggaagacgtt ttttcaccaa
     1861 tacccctccg ttttagtctt tttccgcggc tttccgcatc tcgtcgcgcc gccgctcggc
     1921 ctcttcgttc cgttcggcca acaactcgcg ctgttgaagg cgacgctcct cgatctcctt
     1981 ctccatcaac ctttttttta cgatcttgtc gtacgtctcc gcggattttt ttaccgtctc
     2041 gtgatgcctg tccatgatct caaggcgctc cgtctcgact gcgcgcttcg ccttctcgac
     2101 cttcttcagc ccctcttcct tcgtctcttc gaccttttct tcgacaatgt cctcgacggg
     2161 aagaggggga agaggccgcg tcgtaccaac ggaattgacg ttcatcggca tccccccctt
     2221 tcagtgtttc acaaaagctg cttatgtatc ggcatcctgc gggcgcgtta taaaccggcg
     2281 gccgagagcg aaagcgctcc
//
LOCUS       DAOTIL010000421         2298 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminobacteriaceae bacterium isolate AMR_MDS_3026
            NODE_55530_length_2298_cov_2.839055, whole genome shotgun sequence.
ACCESSION   DAOTIL010000421 DAOTIL010000000
VERSION     DAOTIL010000421.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868050
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminobacteriaceae bacterium (wastewater metagenome)
  ORGANISM  Aminobacteriaceae bacterium
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae.
REFERENCE   1  (bases 1 to 2298)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2298)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:19:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,224
            CDSs (total)                      :: 3,198
            Genes (coding)                    :: 3,184
            CDSs (with protein)               :: 3,184
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2298
                     /organism="Aminobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_55530_length_2298_cov_2.839055"
                     /isolate="AMR_MDS_3026"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:3034609"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..970
                     /locus_tag="P5201_08985"
     CDS             <1..970
                     /locus_tag="P5201_08985"
                     /inference="COORDINATES: protein motif:HMM:NF037660.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="LuxR C-terminal-related transcriptional
                     regulator"
                     /protein_id="HRV98712.1"
                     /translation="MGECMPLYEKLSNGHGAGADSVMRAEACLYRGEETEAEAASHKA
                     LYQARSARQTSISLCAELVLARLAVLRGDGEAYATARKSLCGYRKEFPERSVVRMVEL
                     SLASLDLLLGRTEGIPDWLRDVESIKKTLYIQGHACGLILYGKLLLLEKRFPELYGLT
                     EPAMEFAYGMNYELPRLCQHICLAAAYTEEGREDEAENHLSRALRIALPDRIYLPFAE
                     LGTTLLPLLENAKAGFNMKQMNSLIALCKRHSSGAASVRRHLDPSTALLTPREREIAL
                     LAKERCSTREIASRLFISENTVKSALKIIYGKLEVHSKNELATMDF"
     gene            1088..1375
                     /locus_tag="P5201_08990"
     CDS             1088..1375
                     /locus_tag="P5201_08990"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2442 domain-containing protein"
                     /protein_id="HRV98713.1"
                     /translation="MRVQTGYIRSVKALPGYKLEVEMTTDTRIEFDFASRLNTIRFGA
                     LKDEKLFSTASTDGNFITFGTNDAPKVKISAIDFMDLVLVDRTGRYPPDEN"
     gene            1541..2011
                     /locus_tag="P5201_08995"
     CDS             1541..2011
                     /locus_tag="P5201_08995"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRV98714.1"
                     /translation="MKKTLVKTAGLFVLAALLAVHCASRAEAWIKITLKNNRSHNIAV
                     AFCWSGFDYADDQRKGWYVVQPNDSRVINLANATSSLTWEDFGFYAQGTLRNGKKIRW
                     SGDMKQVIIHPSKAFDGHPEDPIAGGKKVGFRKLNLKKVGDANTDAVATLTFNP"
     gene            2026..2208
                     /locus_tag="P5201_09000"
     CDS             2026..2208
                     /locus_tag="P5201_09000"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRV98715.1"
                     /translation="MQLWAAALLFVLFAAAAFSFFKGGKNKSRIRFYTGILFAFIGGV
                     ALAYIAATVFFVVSIE"
BASE COUNT          581 a          604 c          630 g          483 t
ORIGIN      
        1 tatgggagaa tgcatgccat tgtacgaaaa gttgtccaac ggtcacggcg ccggggcgga
       61 cagcgtaatg cgggccgaag cctgcctgta ccggggcgag gagaccgaag cagaggcggc
      121 aagccataaa gcgctctacc aggcccggag cgctcgtcaa acctccatct ccttatgcgc
      181 ggaacttgtg cttgcgcgcc ttgcagttct gcgaggcgac ggagaggcat acgcgacggc
      241 ccgcaagagc ctgtgcggat accgaaagga atttcccgaa agatccgtcg tccgcatggt
      301 cgaactctct cttgcctcgc tcgatctctt actggggaga accgagggaa tccccgattg
      361 gcttcgcgac gttgaaagca tcaagaaaac gctctatatc cagggacacg cctgcggcct
      421 catactctac gggaagctgc tgcttctgga aaagcgtttt ccggagctgt acgggctgac
      481 ggaaccggcc atggagtttg cgtacgggat gaactatgag ctcccccgcc tctgtcagca
      541 tatctgcctc gccgccgcat acacagagga aggacgcgag gacgaggcgg agaatcatct
      601 ttcccgggcg ctccggatcg cgcttccgga ccggatatac ctgccgttcg cggagctcgg
      661 gacgacgctt ctgcctcttc tggagaacgc gaaagccggc ttcaatatga agcaaatgaa
      721 ctccctgatc gcgctctgca agaggcattc ttccggcgcc gcatccgtac gaagacacct
      781 tgatccctcc acagctcttc tgacgccgcg ggaacgagag atcgccctgc ttgcaaaaga
      841 gcgctgcagc acccgcgaaa ttgcatcccg tctgttcatt tcggagaaca ctgtcaagtc
      901 ggcgctcaaa atcatttacg gcaaactgga agttcactca aagaacgagc ttgccacgat
      961 ggacttctaa cgagcgtaga acaccacccc ccgttccccc ccgaaagagg gggtgtagaa
     1021 caagcagttt tccttcaaaa tacttatgga ctctgcggga aaggctcttc tacaggggtg
     1081 tgagaaaatg agggtacaaa caggatatat cagaagcgtc aaagccctgc cggggtacaa
     1141 gctcgaagtt gagatgacaa cggacacccg tattgaattc gatttcgcct ccaggcttaa
     1201 tacaatacgt ttcggcgcgc tgaaagacga aaaactcttc tccacggcct ccacggacgg
     1261 aaacttcatc acgttcggga caaatgatgc gccgaaggtc aagatttcgg cgatcgattt
     1321 catggacctt gtcctcgtgg acaggacggg cagatatccc ccggatgaaa attagttttt
     1381 tgatgtacct cgttctcatt acggctatgc accaagcaaa actgctcaca gggaaatctt
     1441 gagcactgcg cctttttttc ttttccggta ttcggaggac gccggaatat atgctgttcg
     1501 tcgatacccg gtcaagcggg taagaaaagg gaggaatacg atgaaaaaga cactggtaaa
     1561 aacagcgggg cttttcgtcc ttgcggcgct cttggcggta cactgcgcct ccagagcaga
     1621 agcctggatc aaaataaccc tgaaaaacaa cagatcgcac aacatagcgg tcgccttctg
     1681 ctggagcgga ttcgattatg cggatgatca gagaaaaggc tggtacgttg tccaacccaa
     1741 cgattcaaga gttatcaatc tggcaaatgc cacttcttcg ttgacatggg aggacttcgg
     1801 tttctatgcg cagggaacac tgagaaacgg gaaaaagatc agatggtcgg gtgacatgaa
     1861 acaggtgatt atccacccca gtaaagcgtt cgacggacat cctgaagacc ctatcgcggg
     1921 tggcaagaag gtgggcttcc gcaaactcaa tttgaaaaaa gtgggagacg caaatacgga
     1981 cgcggtagca accctgacgt ttaatccctg acggcgacac aaggtatgca actctgggca
     2041 gcggcgcttc tgttcgtcct tttcgcggcg gcggcgttct ctttttttaa agggggaaag
     2101 aacaaaagca gaatacgctt ctacacgggc attctcttcg cgttcatcgg cggtgttgca
     2161 ttggcgtaca tcgcggcgac agtcttcttc gtagtttcga tagagtaagc gagtacggga
     2221 aacggcagtc gcaggcatgc cttcgcattc atgttgaatc gaaacgaaat gcgaacatat
     2281 tcaaagaaag aggtggtt
//
LOCUS       DAOTIL010000422         2297 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminobacteriaceae bacterium isolate AMR_MDS_3026
            NODE_55556_length_2297_cov_3.217663, whole genome shotgun sequence.
ACCESSION   DAOTIL010000422 DAOTIL010000000
VERSION     DAOTIL010000422.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868050
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminobacteriaceae bacterium (wastewater metagenome)
  ORGANISM  Aminobacteriaceae bacterium
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae.
REFERENCE   1  (bases 1 to 2297)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2297)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:19:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,224
            CDSs (total)                      :: 3,198
            Genes (coding)                    :: 3,184
            CDSs (with protein)               :: 3,184
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2297
                     /organism="Aminobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_55556_length_2297_cov_3.217663"
                     /isolate="AMR_MDS_3026"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:3034609"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1177)
                     /locus_tag="P5201_09005"
     CDS             complement(<1..1177)
                     /locus_tag="P5201_09005"
                     /inference="COORDINATES: protein motif:HMM:NF001989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cobyric acid synthase"
                     /protein_id="HRV98716.1"
                     /translation="MIQGTSSSVGKSVLVAALCRIFARRGLKVAPFKAQNMALNSFVT
                     SAGEEMGRAQAVQAAAAGRAPEVEMNPVLLKPEGNSRSQVVLMGKPWKTLSAGEYYAC
                     REVLWGSVVASFEKLASENDLVLVEGAGSPAEINLKKHEIVNMRVARTFDCPVLLAGD
                     IDRGGVFASLVGTLALLDEEERALVKGFLINKFRGDVKLLEPGLDMLSGLTEGRPVLG
                     VVPWLKNLAVAQEDSVWLEERTAGDSSGGVDIAVIKLPRISNYDDFDPLALEEGVSLR
                     FVERPEDLGHPAVVILPGSKTTLKDLAWLRSSGFDGLIAALALAGGSVVGVCGGYQML
                     GRRISDPLGVEGPASEESGLGLLPVETVFEPAKETTLSRGRTMPSSESPSSGFLAHPF
                     "
     gene            1376..1855
                     /locus_tag="P5201_09010"
     CDS             1376..1855
                     /locus_tag="P5201_09010"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRV98717.1"
                     /translation="MNKRSVLLRFLAFLCVVSLLAPAAFAAQQIPPEVKSYVIKFFNE
                     YGNATKTMADRVQKTDDPEQIAGALDKYVDTVAPLMKGMDDLQKKYKDFFDAMEEEDR
                     EDSGDADIDKANAEFEKTQQGMMDAMQKIASHMEHPKVEAAMERLEKIMSDMDETAE"
     gene            complement(1940..>2297)
                     /locus_tag="P5201_09015"
     CDS             complement(1940..>2297)
                     /locus_tag="P5201_09015"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF014566.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0071586 - CAAX-box protein processing
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HRV98718.1"
                     /translation="LALVWTLVAFLEEGIFRGFLMTEIVKVAGTGTLALGFNVLFTSV
                     VFGLAHGYQNRSGMVSAGMVGLFLGALFIFEGFNLWLVIFVHGFVDTIGIVLIAAGAD
                     RSIDGFIRRLWRMNPH"
BASE COUNT          510 a          772 c          659 g          356 t
ORIGIN      
        1 cgaacgggtg cgcaagaaaa ccggaggagg ggctctccga agacggcatc gttcgccctc
       61 tggaaagcgt cgtctccttc gccggttcga agaccgtctc gacgggcagc agaccgagcc
      121 cggactcctc cgacgcgggg ccctccacgc cgagcgggtc ggaaatgcgc cgtccgagca
      181 tctggtagcc gccgcagacg ccgacgacgg aacctccggc gagcgcgagg gcggcgatca
      241 gtccgtcgaa gccggaactg cgcagccagg ccaggtcctt cagcgtcgtc ttgctccccg
      301 gaaggataac gacagccgga tggcccaagt cctccggacg ctccacgaaa cgcagcgaga
      361 ccccctcttc aagcgcgagc gggtcgaagt cgtcgtagtt ggagatgcgg gggagcttga
      421 tcacggcgat gtccacgccg ccggaggaat cgcccgccgt gcgctcttca agccacacgg
      481 agtcctcctg cgccacggcg aggttcttca gccacggaac gacgccgagc accggacgcc
      541 cctccgtgag accggaaagc atgtcgaggc ccggttccag cagcttcacg tccccacgga
      601 acttgttgat caggaacccc ttcaccagcg cgcgctcctc ctcgtcgagg agcgcgagcg
      661 tcccgacaag cgaggcgaaa accccgcccc ggtcgatatc ccccgcgaga aggaccgggc
      721 aatcgaacgt gcgcgccacg cgcatgttca cgatctcgtg cttcttcagg ttaatctccg
      781 ccgggctccc cgctccttcg acgaggacga ggtcgttctc cgaggccagc ttctcgaacg
      841 acgcgaccac agaaccccac aaaacctccc tgcaggcgta gtactcgccc gccgacagcg
      901 tcttccacgg tttccccatc agcaccacct gcgagcggga attcccctcc ggcttcagca
      961 gcacggggtt catctccacc tccggagcgc gccccgcggc ggcggcctgc accgcctgcg
     1021 cacgccccat ctcctccccc gcggaggtga cgaaagagtt cagcgccatg ttctgcgcct
     1081 tgaacggggc cactttcagc cccctccgcg cgaagatgcg gcagagcgcc gccacgagga
     1141 cgctcttccc cacggaagag gacgttccct gaatcatcag tctggacgca cgaatagcaa
     1201 ttcctccgat ccggtgaatt cctggacaat cctacccagt agcgggcgtt ttggcaagcc
     1261 gaggagcgga acaagcggcg tgcgaaaacc taaaaaagca cgcagtacga ttgctttcct
     1321 ctcgcgagat tgtgatacaa tgagcgccat cttaccgaaa gaaagaggtg tacccatgaa
     1381 taaacgctcc gttcttctcc gtttcctggc atttctctgt gtcgtttcgc tcctcgcccc
     1441 ggcggcgttc gctgcgcagc agattccgcc cgaggtcaaa agttacgtga tcaaattctt
     1501 caacgagtac gggaacgcca caaaaaccat ggcggacagg gtacaaaaaa ccgacgaccc
     1561 cgagcagatc gcaggcgcgc tcgacaagta cgtcgacacg gtcgcgccgc tgatgaaggg
     1621 catggacgac ctgcagaaga agtacaagga cttcttcgac gcaatggaag aggaggaccg
     1681 ggaagacagc ggagacgccg atatcgacaa ggccaacgcc gagttcgaaa agacgcagca
     1741 aggaatgatg gacgccatgc agaagatcgc ctcgcacatg gaacacccga aggtggaggc
     1801 cgctatggag cgactcgaaa agatcatgtc cgacatggac gagaccgcgg aataagcgtt
     1861 tcctgaaaaa gcccctcacc gaaagagtaa atataacggc ccggccgctc ttcacgccgg
     1921 gccgtttcat tgaaatcgct cagtgcggat tcatcctcca gagcctccgg atgaatccgt
     1981 cgatgctccg gtccgctccc gccgcgatca ggacgatacc gatcgtgtcg acgaagccgt
     2041 gcacgaagat caccagccac aggttgaacc cctcgaagat gaagagcgct cccagaaaga
     2101 gccccaccat ccccgcgctc accatgccgc tgcggttctg atagccgtgc gccaggccga
     2161 agacgacgga ggtgaagagc acgttgaagc cgagtgcgag cgttcccgtt ccggcaacct
     2221 tcacgatctc ggtcatcagg aacccccgga agattccctc ctccagaaag gcaaccagcg
     2281 tccacaccag cgcgagc
//
LOCUS       DAOTIL010000423         2289 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminobacteriaceae bacterium isolate AMR_MDS_3026
            NODE_55790_length_2289_cov_3.742167, whole genome shotgun sequence.
ACCESSION   DAOTIL010000423 DAOTIL010000000
VERSION     DAOTIL010000423.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868050
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminobacteriaceae bacterium (wastewater metagenome)
  ORGANISM  Aminobacteriaceae bacterium
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae.
REFERENCE   1  (bases 1 to 2289)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2289)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:19:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,224
            CDSs (total)                      :: 3,198
            Genes (coding)                    :: 3,184
            CDSs (with protein)               :: 3,184
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2289
                     /organism="Aminobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_55790_length_2289_cov_3.742167"
                     /isolate="AMR_MDS_3026"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:3034609"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1829)
                     /locus_tag="P5201_09020"
     CDS             complement(<1..1829)
                     /locus_tag="P5201_09020"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:NF014763.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HRV98719.1"
                     /translation="MPANLRSIRGVLHFYTLLLAVAMIIFPVLIGVYDLYKASRTVER
                     DIAYALDMQSVALQRWFAIHQNNIASIASLPSVQTKAYDDMFEDFQSFVRTNTNFDSI
                     VYVNTEGRGVVNSTTGFNPRPETDVSDRDYYKDAVKGKPHISDILIGRTSGMPVVIFS
                     APLVGDDGFQGLVFGAIRFDTLLNTILSTHFGATGQFLLLNSKGRSLQGTGESSFPNE
                     TLAALLSSDGYSVSYRDASGKRYLAKGKKLEKTNFFLVAQMDYGEFIAPFIGNMVYFV
                     TVSAVLLFFMLSISRKLYKKVDRSLNLLFEGVIKTEQGEYDSLDPELLEEAPFELREL
                     GIAFSSMAETVRSKTAELEFRSFHDELTGLYNRAYFEDAMRRFDSGRFNPVTVVVCDV
                     NGLKMINDTLGHKAGDELIVAAAHSLANAGRKTDIVARIGGDEFAMLLPESTEETAGE
                     VLRRIRAEIDKYKRGEDSLPLNIACGAATTSGRIQNIEALLSEADKQMYASKQLEKPH
                     SKREILEYLSSRIHDLRKASHDRKRHMAACEAIMEAFVREQPDIAPEKKEFLILLAKN
                     HDIGFTEIPSEITEKPGPLTDEEYLLVQRHPETGARIASLFPEL"
     gene            2007..>2289
                     /locus_tag="P5201_09025"
     CDS             2007..>2289
                     /locus_tag="P5201_09025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014163888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6305 family protein"
                     /protein_id="HRV98720.1"
                     /translation="MKRIILCVVAAALFLSGGLTLPAMVSSAAASEIVLTSVGQSPDA
                     TMIRVVLRKMKINAENQPLLRPEGLAGEKILVAVVGGSSKGLGAAGIDKG"
BASE COUNT          519 a          573 c          630 g          567 t
ORIGIN      
        1 agttccggaa agagcgaagc gatacgcgcc cctgtctccg ggtggcgctg gacgagcagg
       61 tattcttcgt ccgtcaacgg tcccggtttt tcggtaatct ccgacgggat ctcggtaaac
      121 ccgatgtcgt ggttttttgc gaggagaatg aggaactctt ttttctcggg agctatgtcc
      181 ggctgctctc ggacgaacgc ttccatgatc gcctcgcacg ccgccatgtg tctctttctg
      241 tcgtgagacg ccttccggag gtcgtgtatt ctggaagaga gatactccag aatttctcgt
      301 ttggagtgcg gtttttcaag ctgtttggat gcgtacatct gcttgtcggc ctcacttaaa
      361 agagcctcga tattctgtat ccggcctgag gtggttgccg ccccgcacgc tatgttcagc
      421 ggaagcgaat cttcgccgcg cttatacttg tcgatctcgg cccgaatacg ccggagtacc
      481 tctcctgctg tttcttcagt agattcggga aggagcatcg cgaattcgtc gcctccaatc
      541 ctggcgacga tatccgtttt ccggccggca ttcgccaacg agtgcgccgc tgcgacgata
      601 agctcgtcgc cggccttgtg tccgagagta tcgttgatca ttttgaggcc gttcacatcg
      661 cagaccacaa cggttacagg attgaaccgg ccggagtcga agcggcgcat agcgtcttcg
      721 aaatacgcgc ggttatacag gccggtcaat tcatcgtgga aggagcgaaa ctccagctca
      781 gcagtcttgc ttcgcactgt ctccgccatc gagctgaagg cgattccgag ctcccgcagt
      841 tcaaaggggg cctcctccag cagctcaggg tcgagagagt catactctcc ctgttcggtt
      901 tttattaccc cttcgaagag caggttgagc gatctgtcga ccttcttgta cagcttgcga
      961 gagatagaga gcatgaaaaa aaggaggact gctgaaaccg taacgaaata gaccatgttg
     1021 ccgatgaatg gagcaatgaa ttcaccgtaa tccatttgag ctacaaggaa aaagttcgtt
     1081 ttttccagtt tcttgccctt ggcgagatat cttttgcccg aagcgtcgcg gtagctcacc
     1141 gaatatccgt cggatgagag gagagccgcg agcgtctcgt tgggaaaact gctttctccc
     1201 gttccctgca aagatctgcc cttggaattc aggaggagaa actgacctgt cgctccgaag
     1261 tgtgttgaca gtatggtatt caaaagggtg tcgaaacgta tggcgccgaa aacaagtccc
     1321 tggaagccgt cgtctcccac aagcggagcc gagaaaataa cgacgggcat gcccgatgtc
     1381 cggccgatga gaatgtcgga gatatgaggc ttccctttga cggcgtcctt ataatagtcg
     1441 cggtcggaaa catcggtttc ggggcggggg ttgaagcctg tagtgctgtt gacaacgccc
     1501 cttccttccg tgttgacata gacgatggaa tcaaagttcg tatttgtacg aacaaaactt
     1561 tgaaaatctt cgaacatatc gtcgtatgct tttgtctgga cggacggaag gcttgcgatg
     1621 gaggcgatgt tattctggtg tatggcgaac catcgctgga gcgccacaga ctgcatatcc
     1681 agcgcgtacg cgatatcccg ctcaacagtc ctcgaggcct tgtagagatc gtacactcct
     1741 atgagcaccg gaaagatgat cattgcaact gcaagaagaa gcgtataaaa gtggagcacg
     1801 ccgcgaatac ttcgtaaatt agcgggcact tgcatgtacc tcccgtcttc ttttttctat
     1861 tcaaataata ccatagggtg ctgtataccg cagccttcga tgtttctatt gaaaagaatg
     1921 ataagaaata tggacagaaa actcctgaag ggtacactta aaaagaacgt atgaatcgta
     1981 tattctattt ttgaggagga tgtgccatga aaagaataat tctatgcgtg gtggcggctg
     2041 ctctgttcct ttccggtggt cttacgcttc cggctatggt ctcttcggcg gcggcttcgg
     2101 agatcgtgct cacctcagta ggacagagtc ctgatgcgac aatgattcgc gtggtgcttc
     2161 ggaagatgaa gatcaacgcg gaaaaccagc ccctgctcag acctgaaggc cttgccggcg
     2221 agaaaattct cgtcgccgtt gtcggaggaa gctccaaggg gctcggcgcg gcgggcatcg
     2281 acaagggtt
//
LOCUS       DAOTIL010000424         2285 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminobacteriaceae bacterium isolate AMR_MDS_3026
            NODE_55937_length_2285_cov_2.793274, whole genome shotgun sequence.
ACCESSION   DAOTIL010000424 DAOTIL010000000
VERSION     DAOTIL010000424.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868050
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminobacteriaceae bacterium (wastewater metagenome)
  ORGANISM  Aminobacteriaceae bacterium
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae.
REFERENCE   1  (bases 1 to 2285)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2285)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:19:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,224
            CDSs (total)                      :: 3,198
            Genes (coding)                    :: 3,184
            CDSs (with protein)               :: 3,184
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2285
                     /organism="Aminobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_55937_length_2285_cov_2.793274"
                     /isolate="AMR_MDS_3026"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:3034609"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..615)
                     /locus_tag="P5201_09030"
     CDS             complement(<1..615)
                     /locus_tag="P5201_09030"
                     /inference="COORDINATES: protein motif:HMM:NF023583.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008170 - N-methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006306 - DNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-6 DNA methylase"
                     /protein_id="HRV98721.1"
                     /translation="MITGEIKSRIDRIWDTMWSGGISNPLSVIEQLTYLLFIKRLDEL
                     HTLKERKAAHFGMPLEGPIFSPGQEHLRWSRFKETAPEQMFAVVRDEVFPFIKGLGRN
                     GSDGNGSTYSHHMKDAIFMMPAPRVLANVVDQLDAIDMADADTKGDLYEYMLGKIATA
                     GQNGQFRTPRHIIKMMVDMTAPTPRDVICDPACGTAGFLIAASEY"
     gene            complement(724..1407)
                     /locus_tag="P5201_09035"
     CDS             complement(724..1407)
                     /locus_tag="P5201_09035"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRV98722.1"
                     /translation="MTFIADEAAFDAPAFGIIGSPLFNAPPSLGKMAAGGEWERAEVF
                     QPGRFISEEEVKKYLGKKEEIACEAFFGDAFFGARKRGTPEELVRFVLDFNRALASSA
                     NGRDFFRRVAERPGIPVGSGFLFAEVGAVDAWKSVGPFRIEDPCAALEHFEDLLSKLE
                     RSPAGCEREHNKAVEFAFDGGRCEHWIALPVSEGPVIVPSMLEDALRKWCESSERERL
                     PVNSKNKKA"
     gene            complement(1404..>2285)
                     /locus_tag="P5201_09040"
     CDS             complement(1404..>2285)
                     /locus_tag="P5201_09040"
                     /inference="COORDINATES: protein motif:HMM:NF012772.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HRV98723.1"
                     /translation="KKISNALDKAYDMLENDPLRFTMHGQTETADFLRFLALDLENDL
                     QTEKLRLHYQPQVDHDGRVFGVEALLRWTHENYGPVAPQLAVRLAESTGLIGKLGYWI
                     LNRACSDLARVRSTGRTDIVFSINVSPIQLEDEHFAQTLLALIEKHAVDPAALKIEIT
                     EESALANSARVIEQLKTLVQHGVRLAMDDFGMGHTSLKYMREYSFDTIKIDGSIVREV
                     TGDKKCADIIASIVSLGAAMSFSVVAEFVENEEQMKALQTLGCLQYQGYLFGKPVPFE
                     EMFAAVQKEPARFGELP"
BASE COUNT          419 a          660 c          713 g          493 t
ORIGIN      
        1 gtactccgag gcggcgatga ggaagcccgc cgtgccgcac gcggggtcgc agatgacgtc
       61 gcggggcgtg ggcgcggtca tgtccaccat cattttgatg atgtggcgcg gggtgcggaa
      121 ctgaccgttc tgcccggcgg tagcgatctt gccgagcatg tattcgtaga ggtcgccctt
      181 ggtgtcggcg tctgccatat caatggcgtc gagctggtcg acgacgttgg ccagcacgcg
      241 gggcgcgggc atcatgaaga tggcgtcttt catgtgatgg ctgtaggtgc tgccgttacc
      301 gtccgatccg ttgcgcccca gccccttgat gaagggaaat acctcgtcgc gcacgacggc
      361 gaacatctgc tcgggcgcgg tctccttgaa gcgcgaccag cggagatgct cctgtccggg
      421 agagaatata gggccttcga ggggcatgcc gaagtgggcg gccttgcgct ccttgagcgt
      481 gtgcagctcg tcaaggcgct tgatgaacag gaggtaggtc aactgttcga tgacggaaag
      541 ggggttggat atccctcccg accacatagt atcccaaatt cggtcgattc gagatttgat
      601 ttctccggtg atcatagggc gcctcctgtg cgacggcatg ccggttcgac tgcgccgggc
      661 ggcgttccga tgggcatgcg cgtgcaatct gtgaacgatg tcacactact atagcacgtt
      721 ttttcaggct tttttgtttt tggaattcac ggggaggcgt tctctttcac tcgattcgca
      781 ccacttccgc agcgcgtcct caagcatgga ggggacgatg acggggcctt cggagacggg
      841 gagggcgatc cagtgttcgc agcggccgcc gtcgaaggcg aactcgacgg ccttgttgtg
      901 ttcgcgctcg cagcctgcgg gggagcgttc cagcttgctc aacagatctt cgaagtgttc
      961 cagtgcggcg caggggtctt cgatgcggaa cgggccgacg ctcttccatg cgtccacggc
     1021 gccgacttcg gcgaagagga agccgctccc tacaggaatc cccggcctct cagcgacgcg
     1081 ccggaagaag tcgcggccgt ttgcggatga agcgagcgca cggttgaagt cgaggacgaa
     1141 gcgtacaagc tcctccggcg ttccgcgctt ccgagcgccg aaaaaggcgt cgccgaagaa
     1201 ggcttcgcag gctatctcct cttttttgcc gagatatttt ttcacttctt cctcggaaat
     1261 aaagcgtccc ggctgaaaga cttccgcacg ctcccattcg cccccggcgg ccatcttccc
     1321 aagcgagggc ggcgcgttga aaagagggct ccctatgatg ccgaatgcgg gagcatcaaa
     1381 cgcagcttca tcggcaatga atgtcacggc agctctccaa accgggcggg ctctttttgc
     1441 acggctgcga acatttcctc gaagggaacg ggcttgccga aaagataccc ttggtattga
     1501 aggcacccca gcgtttgaag cgccttcatc tgctcctcgt tttcgacgaa ctccgcgacg
     1561 accgaaaagg acatcgccgc gcccagggag actatggagg cgatgatatc ggcgcacttc
     1621 ttgtcgcccg tcacttcgcg gactatggag ccgtcgatct tgatggtgtc gaacgagtac
     1681 tcccgcatgt atttcagaga ggtatgcccc atcccgaagt cgtccatcgc cagcctgact
     1741 ccgtgctgca cgagcgtctt caactgctcg atcacccgtg cgctgttggc gagcgcggac
     1801 tcttcggtga tctctatttt gagggcggcg ggatcgaccg cgtgcttttc gataagcgca
     1861 aggagcgtct gtgcaaagtg ctcgtcctcc agctgaatcg gggagacgtt gatggagaag
     1921 acaatgtccg ttctccccgt tgagcgcacc cgggcgaggt cggaacatgc ccggttgagt
     1981 atccagtagc ccagctttcc gataagccct gtggattcag ccagccgaac ggccagttgc
     2041 ggcgcaacgg gaccgtagtt ttcgtgcgtc caccgcagca gggcttccac tccgaagacg
     2101 cgcccgtcgt gatccacctg gggctgatag tgcaggcgca gcttttcggt ctggaggtcg
     2161 ttctcaagat ccagcgccag gaaacggaga aaatccgccg tttccgtctg cccgtgcatc
     2221 gtaaagcgca acggatcgtt ctctagcata tcgtaggctt tgtccagcgc gttcgagatt
     2281 ttttt
//
LOCUS       DAOTIL010000425         2283 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminobacteriaceae bacterium isolate AMR_MDS_3026
            NODE_56001_length_2283_cov_2.808348, whole genome shotgun sequence.
ACCESSION   DAOTIL010000425 DAOTIL010000000
VERSION     DAOTIL010000425.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868050
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminobacteriaceae bacterium (wastewater metagenome)
  ORGANISM  Aminobacteriaceae bacterium
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae.
REFERENCE   1  (bases 1 to 2283)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2283)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:19:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,224
            CDSs (total)                      :: 3,198
            Genes (coding)                    :: 3,184
            CDSs (with protein)               :: 3,184
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2283
                     /organism="Aminobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_56001_length_2283_cov_2.808348"
                     /isolate="AMR_MDS_3026"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:3034609"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(107..1483)
                     /locus_tag="P5201_09045"
     CDS             complement(107..1483)
                     /locus_tag="P5201_09045"
                     /inference="COORDINATES: protein motif:HMM:NF013073.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SH3 domain-containing protein"
                     /protein_id="HRV98724.1"
                     /translation="MKRIHSFLHRCKKMWFGGGGAACALAFLCCAFFPGLTGAHSAWA
                     LAGLEVPQSPMYHAASFMDEPLITAERHQELYRRLKEQFYSPWSQTGPRQATEVLEWV
                     FDRYGVNVYGENLQPRSAAWIEEQRRQARLDAVGELNRHAVSVRPTSLRLMPTETPAF
                     RSPELAGEGYPFDYLQNSAVHPGEPLFVSHLSLDGLWAWCDTSYASGWVEVRDLAFVD
                     DAAKVRWTALPLAAIVRENALLRDGAESLFRAKVGALLPLLRRGIGEHALIVPVRGQN
                     GLMTERAIRISAEEAVPAPLVPTTWTLAAVAEELRGELYGWGGFLGNRDCSATTRDTL
                     LPFGVWLPRNSRFQAESGRFLTLEGLSNEEKKELLKREGVPFLTLVGLPGHIMLYVGT
                     HRGEPLVFHNMWGIRTERKGVEGRYVLGRTAVTTLDLGEDLPEHAPGKLLIDRINRLG
                     FPAEDGVK"
     gene            1708..>2283
                     /locus_tag="P5201_09050"
     CDS             1708..>2283
                     /locus_tag="P5201_09050"
                     /inference="COORDINATES: protein motif:HMM:NF024970.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HRV98725.1"
                     /translation="MNIPMGATMEMRFPLLQDFLPTILSCCREISGLFGFSHKDTLRL
                     EIALEEALTAIIPGESSDPLPKSLTVRFRRVPLGIRVILGEFHAPLDRKSMDAYSPGE
                     EPDERDFSGLGLFLMRNLVDQAAFYDFEAEGRGKELHLFKMLRREADEPLPQLPLAAI
                     PEKNEPAVREKPFSSERVAPSRVSAITSSFAH"
BASE COUNT          445 a          772 c          643 g          423 t
ORIGIN      
        1 caggactcgt tgaacggacg aactgattct cttccgcccc cggcggaacg gccggtctga
       61 cgcaagggag cctcgtatga ccacatccgc cgaggctccc ttgtgtttat ttcactccgt
      121 cctccgcggg aaagcccaga cggttgatcc tgtcgataag cagctttccc ggagcatgct
      181 cgggaagatc ctccccgaga tcgagcgtgg tcaccgccgt ccgtccgaga acataccgcc
      241 cttcaacacc ctttcgctcc gtccgtatcc cccacatgtt atggaagacg agcggctccc
      301 ccctgtgcgt tccgacgtag agcatgatat gccccggaag accgaccagc gtcagaaagg
      361 gaaccccttc ccgcttcaga agttctttct tttcctcgtt cgagagtcct tcgagcgtga
      421 gaaatcgccc gctctccgcc tgaaaacgcg agttgcgggg aagccagacg ccgaagggaa
      481 gcagtgtatc tcgcgttgtt gcgctgcagt cgcggtttcc taaaaaaccg ccccagccgt
      541 aaagttcgcc gcgcagctcc tccgccaccg cggcaagcgt ccacgtcgtg gggacgagcg
      601 gcgcggggac ggcctcctcg gcggagattc ggattgcgcg ctcggtcatc agaccgttct
      661 gtcctcgcac cgggacaatg agcgcatgct ccccgatgcc tcgccgcagc agcggcagca
      721 gcgcgccgac cttcgcccgg aagagcgact ccgctccgtc gcggagcagc gcgttctccc
      781 gcacgatcgc cgcgagaggc agcgccgtcc acctgacctt cgcggcatcg tcgacgaaag
      841 cgagatcccg aacctccacc caccccgacg cataggaggt gtcgcaccac gcccacaggc
      901 cgtccagcga gagatgcgag acgaagagcg gctctccggg gtgcaccgcg ctgttctgca
      961 ggtagtcgaa cgggtacccc tcccccgcca gttcagggga gcggaaggcg ggcgtctcag
     1021 taggcatcag gcgaagcgac gtcgggcgaa cgctcaccgc gtgccggttc agctctccga
     1081 cggcatcgag ccgcgcctgg cggcgttgtt cctctatcca tgcggccgaa cgcggctgga
     1141 gattctcacc gtagacgttg acgccgtacc tgtcgaatac ccactccagc acctcggtag
     1201 cctgacgcgg cccggtctgg ctccacggcg aatagaactg ctccttcaga cgacggtaca
     1261 gctcttgatg acgttccgcc gtgatcagcg gttcgtccat gaatgaagcc gcatggtaca
     1321 tcggcgactg cggcacttcc agaccggcga gcgcccacgc ggaatgcgcg ccagtcaagc
     1381 cggggaagaa ggcgcaacac aggaacgcga gcgcgcacgc cgcgcctccg cccccgaacc
     1441 acattttttt gcagcgatgg agaaatgagt gtattctttt caccgaaacg acctccctcc
     1501 ggattgcggc gccctcctca ctcttcgcag ggaccacgcg ccgtcaagag taccgctttt
     1561 ttccgcgggc gtccagacgc aattcccgcc cgcgttttca cgaactgcgg agtgttgaaa
     1621 gaatcttttc gatatgacat actgagcact tgaaccgaac ggtacagata ggctctcgat
     1681 accgcctctt ttgaaaggag ctgcgcgatg aacattccaa tgggcgccac gatggagatg
     1741 cgcttccctc tcctccagga ctttctgccg acgatcctct cctgctgccg cgaaatcagc
     1801 ggcctcttcg ggttttcaca taaagatacg ctgcggctcg aaatagccct ggaagaggcg
     1861 ctcacggcaa tcatccccgg agaatcatcc gaccccctcc cgaagtccct gacggtgcgc
     1921 tttcggagag ttcccctcgg aatcagagtc attctcggcg agtttcacgc cccgctcgac
     1981 agaaagagca tggacgcgta ctccccgggg gaagagccgg atgagcggga tttttccggc
     2041 ctcggcctct ttctgatgcg caatcttgta gaccaggcgg ccttctacga ctttgaggcg
     2101 gaagggagag ggaaagagct gcacctcttt aaaatgctgc ggagggaagc cgacgaaccg
     2161 ctcccgcagc ttcctctggc tgcgataccg gaaaaaaacg agcccgccgt tcgcgaaaaa
     2221 cccttttcct ccgaacgagt cgctccctca cgcgtctccg ccatcacctc ctctttcgcc
     2281 cat
//
LOCUS       DAOTIL010000426         2277 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminobacteriaceae bacterium isolate AMR_MDS_3026
            NODE_56191_length_2277_cov_3.614311, whole genome shotgun sequence.
ACCESSION   DAOTIL010000426 DAOTIL010000000
VERSION     DAOTIL010000426.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868050
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminobacteriaceae bacterium (wastewater metagenome)
  ORGANISM  Aminobacteriaceae bacterium
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae.
REFERENCE   1  (bases 1 to 2277)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2277)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:19:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,224
            CDSs (total)                      :: 3,198
            Genes (coding)                    :: 3,184
            CDSs (with protein)               :: 3,184
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2277
                     /organism="Aminobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_56191_length_2277_cov_3.614311"
                     /isolate="AMR_MDS_3026"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:3034609"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..201
                     /locus_tag="P5201_09055"
     CDS             <1..201
                     /locus_tag="P5201_09055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009166182.1"
                     /note="Converts oxaloacetate to phosphoenolpyruvate using
                     ATP as an energy source; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxaloacetate decarboxylase subunit alpha"
                     /protein_id="HRV98726.1"
                     /translation="RPAELIKPELEDARKAIAPWILQPEDVLSYVLFPSVAKDFLMKK
                     FAREMKVDVGVAELVDGAGYPV"
     gene            302..1234
                     /locus_tag="P5201_09060"
     CDS             302..1234
                     /locus_tag="P5201_09060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008522494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PhoH family protein"
                     /protein_id="HRV98727.1"
                     /translation="MARFAGNNDENLSEIEQRYPVSLLVRGNKVAVRGPDEVAVDVAY
                     GLLEQLHKIASKGHALHLADVRYAMDMIAEKGAVDLAPLFSEVICTTARGKPIRAKTE
                     GQRAYLETVRNNHIVFAVGPAGTGKTFLAVCHAVTLLKASAISRIVLVRPAVEAGESL
                     GYLPGDLREKVEPYVRPLYDAFYDLLSPERFLRYVDKGVIEIAPLAYMRGRTLNDSFI
                     ILDEAQNTTPEQMKMFLTRLGFGSKAVITGDITQIDLPNGKKSGLIQVRDILEGIEGI
                     EFFNLTHSDVVRHEIVQKVVRAYERYEQQREQRA"
     gene            1206..>2277
                     /locus_tag="P5201_09065"
     CDS             1206..>2277
                     /locus_tag="P5201_09065"
                     /inference="COORDINATES: protein motif:HMM:NF019317.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRV98728.1"
                     /translation="MSSSGNSAHKGAGYMGTGKRKNSFLFFTEDASPDLRSLSLLLLV
                     AFALTVIFLKWFFEDRISGFLPGEASSRTYFALYPMKYVDEEGTVALRSRVGDTVSGV
                     LVRDGAAMDRLISKIEIMAEGDVSLLSLSPGLTELIKSYPEEARKRFLQASARLSSAY
                     LQSQGEKPGGFSWSPEILWKEIERLALPMEENNIVYQILDEILQPLSRVDDDLTRMLR
                     KEMGAALQPVERSIVPGDVLVEKGQIITDQAARILEMQGYSRVSFPWKQILFALVVIP
                     FWPLWLRIQTRNHGGGEDKSELPWVYLAFIVGVSWLAEYVSALFHAQGMGSLFLAGCA
                     YLTLPAHLALQLVLGGAILGGLV"
BASE COUNT          469 a          635 c          697 g          476 t
ORIGIN      
        1 cgccctgcgg agctgatcaa acctgagctt gaagatgcgc ggaaggctat cgctccgtgg
       61 attctccagc ctgaggacgt actcagctac gttctcttcc cgtcggtggc caaggacttc
      121 ctgatgaaga aattcgcgcg ggagatgaag gttgacgtgg gcgtcgcgga acttgtcgac
      181 ggcgccggat atcctgtcta aagggagcgt gcatcggaga agaatgtaca ttgatgtaga
      241 aaacaaagcg gaggagaccg gagcatttct gcgcgagctt gatttcagcg acatagagac
      301 tatggcgcgt ttcgcgggga ataatgacga aaatctctcc gagatagagc agcgctatcc
      361 cgtgagcctc cttgtacggg ggaataaagt cgcggtacgc ggtccggacg aggtcgccgt
      421 ggacgtggcg tacgggttgc ttgagcagct ccataaaata gcctcgaagg ggcatgcgct
      481 ccacctggcg gatgtccgct acgcgatgga tatgatagcg gagaaggggg cggtcgatct
      541 ggcccccctc ttctccgaag ttatttgcac cacggccaga ggaaaaccga tccgggcgaa
      601 gaccgagggg cagcgcgcgt accttgagac cgtccgcaat aatcatattg tctttgccgt
      661 cggaccagcc ggaaccggga agacctttct tgccgtctgc cacgccgtga cgctgctcaa
      721 ggcctcggcg atcagcagga tcgtgcttgt gcgtcccgcc gtggaggcgg gggagagcct
      781 cggctatttg cccggcgacc ttcgggagaa agtggagccg tacgtgcgcc cgctttatga
      841 cgcattctac gacttgctct ctccggagcg ctttctccgg tacgtggaca agggggtcat
      901 cgaaatagcc ccgctggcgt acatgcgggg aagaacgctc aacgacagct tcatcatcct
      961 cgacgaagcg cagaacacca cgcccgagca gatgaagatg tttctcaccc ggctcggctt
     1021 cggctccaaa gccgtcatca cgggcgacat cacccagatc gacctgccga acgggaagaa
     1081 gtccggactg atacaagtca gggacatcct tgaaggaatc gagggaattg agtttttcaa
     1141 tctcacgcat tcggacgtgg tccgtcacga gatcgtccag aaagtcgtca gggcgtacga
     1201 acgctatgag cagcagcggg aacagcgcgc ataaaggcgc aggatatatg ggaaccggca
     1261 aacgaaagaa ctctttcctt tttttcaccg aggacgcttc tcccgacctt cgttcgctct
     1321 ccctgctcct tcttgttgcc ttcgcgctga ccgttatttt tttgaaatgg ttctttgaag
     1381 accggatatc tgggttcctg ccgggggagg cctcttcacg gacctacttt gctctgtacc
     1441 cgatgaagta cgtggacgag gaagggacgg ttgcgctccg ctccagggtc ggcgataccg
     1501 tctcgggagt gcttgtccgc gacggcgccg cgatggaccg cctcatctcg aagatagaga
     1561 taatggcgga gggggacgtt tcgctcctct cgctctctcc cggattaacc gagttgataa
     1621 aaagttaccc ggaagaggcg cggaagaggt tcttgcaggc aagcgcccgg ctgagcagcg
     1681 cctaccttca atctcagggg gaaaagccgg gcggcttttc ctggtctccc gaaatcctct
     1741 ggaaggagat cgagaggctg gcccttccaa tggaggagaa caacatcgtg tatcagatcc
     1801 tcgacgagat tctccagccc ctctcgaggg tggacgacga cctcacccgg atgctgcgga
     1861 aagagatggg agcggcgctg cagcctgtgg agcgatccat cgtaccgggc gacgtgctcg
     1921 tggaaaaggg gcagatcatc accgatcagg ccgccagaat cctcgaaatg cagggctact
     1981 ccagggtgtc ttttccgtgg aagcagatac tcttcgcgct tgtggtcatc cccttctggc
     2041 ctctttggct ccgcatccag acgcggaatc acggcggcgg cgaagacaaa agcgaactac
     2101 cgtgggtcta cctcgccttt atcgtcggcg tcagctggct tgcggagtac gtctcggccc
     2161 tttttcacgc ccaggggatg gggagcctct tccttgcagg atgcgcgtat ttgacgcttc
     2221 ctgcgcacct tgcgcttcag cttgttcttg ggggagcaat cctcggcggt cttgtgg
//
LOCUS       DAOTIL010000427         2273 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminobacteriaceae bacterium isolate AMR_MDS_3026
            NODE_56336_length_2273_cov_4.064923, whole genome shotgun sequence.
ACCESSION   DAOTIL010000427 DAOTIL010000000
VERSION     DAOTIL010000427.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868050
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminobacteriaceae bacterium (wastewater metagenome)
  ORGANISM  Aminobacteriaceae bacterium
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae.
REFERENCE   1  (bases 1 to 2273)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2273)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:19:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,224
            CDSs (total)                      :: 3,198
            Genes (coding)                    :: 3,184
            CDSs (with protein)               :: 3,184
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2273
                     /organism="Aminobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_56336_length_2273_cov_4.064923"
                     /isolate="AMR_MDS_3026"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:3034609"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(329..2134)
                     /locus_tag="P5201_09070"
     CDS             complement(329..2134)
                     /locus_tag="P5201_09070"
                     /inference="COORDINATES: protein motif:HMM:NF033559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS1634 family transposase"
                     /protein_id="HRV98729.1"
                     /translation="MNTKKTGSAKAATETIALESINVLTPGSAPVALKLARIGGIGET
                     VDGSVSWDRSRCSLSPGIVAETLVSALLCGCRALYNMKSFWEESGCAAWFERNGVVPE
                     QLNDDVYARLLDRFSECDMRKIVETVAFRLRTAHGIKVSTVHFDTTSVSVEGDYATTK
                     EERGEESDGAETSEPAAADAEKPSFEIERGHSKDRRPDLKQIKIGLAVQEDGIPLTGE
                     LLSGSASDRTWNGDAVGYISELLGGQGVEDAVFVADSALVSKENLAELADGGMDFVTL
                     LPGTFSLEKELKERAWREEKWTDLGALARDPDEKSARYKTRSVREEIEGRTYDFLVVH
                     STKLEALKERSLKSRFDRLGVELRKESLALRKREFACEEDARRGGAELLEKAAKYGIV
                     AGFSVELEEKAVRGKGRPKKDAPPPATNRTWRAVVEIGEVEEKTWKSAMDRESTFILV
                     YRMEKAVERKNPADILRTYKNQNVVEQGFRFLKQPIYLGPVLLKKPERVEALGYVFLL
                     VLLLAKYLEYRVRASLEKEGDALRVGGQKLARPTTQTILYHFGTVPLLESGGKRFMPN
                     PPGGNGTRIMDALGFSWELYTHGDCEDRFMERFRS"
BASE COUNT          428 a          752 c          580 g          513 t
ORIGIN      
        1 cgacggcgtc tccagcagga aatgagtcgc gggatgagaa gtattttctc tccacgaaca
       61 tatcctaccc gtatttcgca cctcagcaaa agagcgtctt gattttcctg cgaacaatgg
      121 acgccttcaa gggaaagcgg cttttcccgc actttgccaa acgaatatcg tcgcgaacaa
      181 cgcccccgag gattcgaacc agccgactcg atgagaatag ggctgtaaaa gtccatacaa
      241 cacacgatcc cgttgaaata acggaatcca tccatccgca gctacagaaa gcttcgcctc
      301 gtcggggcgt tacaacggga tgctcctctc agctgcggaa acgctccatg aagcgatcct
      361 cgcagtctcc gtgcgtgtac agctcccagg aaaaaccgag cgcgtccatg atgcgggttc
      421 cgttacctcc gggcggattc ggcatgaacc gtttccctcc gctctccagc agcggcacgg
      481 ttccgaaatg gtacaggatc gtctgcgtcg taggacgggc gagtttctgt cctccgaccc
      541 gcagcgcgtc gccttccttc tccagcgacg cccgtacccg gtattcaaga tacttcgcga
      601 gaagcagcac gagaagaaag acgtagccca gcgcttccac ccgttccggc ttcttcagca
      661 ggacggggcc gaggtagatc ggctgcttga ggaagcggaa tccctgttcc acgacgttct
      721 ggttcttgta cgtcctcagg atgtccgcgg gattcttccg ctcgacagcc ttctccatcc
      781 gatacacgag aatgaatgtc gactccctgt ccatggcgga tttccacgtt ttctcctcga
      841 cctctcctat ttcgacgacc gccctccacg tacggttcgt cgccggaggg ggagcgtcct
      901 tcttcggtct gcccttgccc cgtacggctt tctcctcgag ctcgacggaa aatccggcga
      961 cgatcccata cttcgccgcc ttctccagaa gttccgctcc gcctcttcgg gcgtcctcct
     1021 cgcaggcgaa ctcccgcttc cgcaacgcga gactctcttt gcgcagttcg acgcccagtc
     1081 tgtcgaaacg gcttttcaga ctcctctcct tcaaggcttc gagcttcgtc gagtggacca
     1141 cgagaaagtc gtacgttctt ccctcgatct cctcccggac gctccgcgtc ttgtagcgcg
     1201 cgctcttttc gtcgggatcc cgggcgagag cgccgaggtc cgtccacttc tcttcgcgcc
     1261 acgctctctc cttgagctcc ttctccagag aaaatgtccc gggaagcagc gtcacgaaat
     1321 ccattcctcc gtcggcgagc tccgccaggt tctccttgct cacgagcgcg ctgtcggcca
     1381 cgaaaacggc gtcttcgact ccctgccctc cgagcagttc cgatatgtac ccgacggcgt
     1441 ctccgttcca cgtccggtcc gaagcgctgc cggagagaag ttctcccgtg agcggaatcc
     1501 catcctcctg caccgcgagg ccgatcttga tctgcttcag gtccggacga cggtccttgg
     1561 agtgcccgcg ctcgatctcg aaagagggct tttcggcgtc cgcggcggcg ggttcggacg
     1621 tctccgcgcc gtcgctctcc tctccgcgct cttccttcgt cgtcgcatag tcgccttcca
     1681 cggagaccga cgtcgtgtcg aaatgcacgg tcgaaacctt gatcccgtgc gccgtcctga
     1741 gcctgaacgc cacggtctcc actattttgc gcatatcgca ttcggagaac ctgtccagaa
     1801 gccgcgcgta cacatcgtcg ttcagctgct ccggcacgac gccgttcctc tcgaaccagg
     1861 cggcgcatcc ggactcctcc cagaaggact tcatgttgta gagcgcccga cagccgcaaa
     1921 gaagagccga tacgagcgtt tctgccacta ttccgggaga gagggagcat cgggaacggt
     1981 cccacgacac gctcccgtcc accgtttctc cgattcctcc tatgcgcgcg agtttcagag
     2041 cgacgggagc ggagccgggg gtgagcacat ttatggattc aagcgcgatt gtctccgttg
     2101 cagcttttgc gctgccagtc ttttttgtat tcatacgacc tattttatgg caaatacgac
     2161 acattgtcac ctgcctcaaa aaatacccga tcattcaaat cgacatgcga aatacgggtt
     2221 caatgcttac tccttgccat ttcctgttgt aaaacatctc gttctttaag cgt
//
LOCUS       DAOTIL010000428         2271 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminobacteriaceae bacterium isolate AMR_MDS_3026
            NODE_56404_length_2271_cov_3.083935, whole genome shotgun sequence.
ACCESSION   DAOTIL010000428 DAOTIL010000000
VERSION     DAOTIL010000428.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868050
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminobacteriaceae bacterium (wastewater metagenome)
  ORGANISM  Aminobacteriaceae bacterium
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae.
REFERENCE   1  (bases 1 to 2271)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2271)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:19:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,224
            CDSs (total)                      :: 3,198
            Genes (coding)                    :: 3,184
            CDSs (with protein)               :: 3,184
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2271
                     /organism="Aminobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_56404_length_2271_cov_3.083935"
                     /isolate="AMR_MDS_3026"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:3034609"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..667
                     /gene="fusA"
                     /locus_tag="P5201_09075"
     CDS             <1..667
                     /gene="fusA"
                     /locus_tag="P5201_09075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013048751.1"
                     /note="EF-G; promotes GTP-dependent translocation of the
                     ribosome during translation; many organisms have multiple
                     copies of this gene; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HRV98730.1"
                     /translation="TPQVPYRETIRKTAEAQGKHKKQTGGHGQYGDAHIRYTPLERGA
                     GFVFEDNIVGGAIPKGFIPAVEKGLRESLVKGPLAGFPVVDFKATLFFGSYHDVDSSE
                     MAFKLAARLSFKKGILDAGPILLEPIMNVEVTVPEDYLGDVMGDFNSRRGRILGIDSK
                     GHQQIVKAQAPLAEMFRYAIILRSMTSGRGSFTMEYDHYEEVPAEITKKVVAAHKQEE
                     EEE"
     gene            757..>2271
                     /locus_tag="P5201_09080"
     CDS             757..>2271
                     /locus_tag="P5201_09080"
                     /inference="COORDINATES: protein motif:HMM:NF013086.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding protein"
                     /protein_id="HRV98731.1"
                     /translation="MKKRVIVVGSGGAGLTAAISARKTGAEVVLLSKTGCAEASCTAY
                     SGGLFSLSSGAVSPDDHYRRIMETGRYVNDPSLVRILADHSEATLRTISEWGVSLKVT
                     TSGHATARKTAPSRIMGGGGMTRELASIARREGVVILENHIASRLLVGTHGVEGVEAV
                     DWKRGKAFSFGASAVILATGGGGAIYERTDNPLRMTGDGYALALDAGLELVDMEYVQF
                     YPMGWDQKGYPVWMVGLGLLDYIPVTDEKGREFLKEAFPLWGVSTGKEANLFARDRAA
                     LLLAGHAVKGGRTLLHLEKFREEDLSLPDVQASLLFDLPPEKRSAPVHVSPVQHYFTG
                     GVPMDSEGRTSIPGLFVCGEVSGGVDGASRMGGNALTNILVFGLRAGKAAAEEAMASS
                     AVSDAVSKLPECVLRSGREGVSPAKVRCELRSIVQEGLSPWRNAEGIERCISKIELLN
                     ASRKALKIASSLDLLHAIELHGLHRTAESVGRAAFARQESRGVHFRDDYPNEREE"
BASE COUNT          495 a          596 c          699 g          481 t
ORIGIN      
        1 gacgccccag gtaccctacc gcgagaccat ccgcaaaact gccgaggccc aggggaaaca
       61 caagaaacag accggcggtc acggtcaata cggagacgct catatccggt acactccgct
      121 cgaaagaggc gcaggatttg tgttcgagga caatattgtc ggcggcgcca ttccaaaggg
      181 attcatcccc gcagtcgaga aagggctccg tgagtcgttg gtcaagggac cgcttgcagg
      241 ttttcctgtg gtcgacttca aggcgacgct cttcttcggc tcgtatcacg acgtagatag
      301 ctccgaaatg gcgtttaaac tcgccgcccg cctttccttt aagaagggta tccttgatgc
      361 gggccccatt cttctcgagc cgatcatgaa tgtcgaagtc accgtgccgg aggactacct
      421 cggggacgtt atgggcgatt tcaacagcag gagaggacgt atcctcggca tcgacagcaa
      481 gggacaccag cagatcgtta aagcgcaggc gcctcttgcg gagatgttcc gctatgcgat
      541 tatccttcgt tcaatgacct ccggaagagg ctccttcact atggagtacg atcactacga
      601 agaagtcccg gccgagatta cgaaaaaggt tgtcgctgcg cacaagcagg aagaggaaga
      661 agaataagct ttttctcaaa cacgagttct caagagagcc ccgcgcgatg cggggctctc
      721 ttgtaataag cacgtttcac aaggaggtcg gatacaatga aaaaaagagt gattgtcgtc
      781 ggttcgggag gagccggtct gactgccgct atctccgccc gcaagaccgg cgcggaagtt
      841 gtgcttctaa gcaagacggg gtgtgcggag gccagttgca cggcctattc cggcggactc
      901 ttctctcttt cgtcgggggc cgtttcaccg gatgatcact accgtcggat aatggaaacg
      961 ggacggtacg tcaatgatcc atcgttggtt cgaatccttg cggatcattc cgaagccacg
     1021 ctcaggacaa tctccgaatg gggagtctcc ttgaaagtca ctacatccgg tcatgcgact
     1081 gcacgcaaga ccgccccttc gagaatcatg ggcggcggag ggatgacccg tgagcttgcc
     1141 tcgatcgcga gacgcgaagg tgtcgtcatt ctggagaatc atatagcttc acgtctgttg
     1201 gtcggtacgc atggcgttga gggagtggaa gctgtcgact ggaagcgggg gaaggcgttc
     1261 tctttcggcg catccgctgt gattcttgcg accggaggag gcggggcgat ctatgaacgg
     1321 acggacaacc cgctgcgtat gacgggagac ggatacgcgc tcgcacttga tgccggtctt
     1381 gaacttgtgg atatggagta cgtgcagttc tacccgatgg ggtgggatca gaaaggatac
     1441 cccgtctgga tggtcggact ggggctgctt gactacattc cggttacgga tgagaaggga
     1501 agagaatttc tcaaggaggc cttcccttta tggggcgtct ccaccggaaa agaagcgaat
     1561 ctctttgccc gcgaccgtgc agcgctctta cttgcgggac atgctgtgaa agggggaagg
     1621 acgctgcttc atctcgagaa gttcagggaa gaagacctct ctctccccga tgtccaggct
     1681 tcgctgttgt tcgaccttcc gccggagaaa cggtccgctc cagtgcacgt cagtccggta
     1741 cagcactatt ttacaggggg cgttccaatg gattccgaag ggagaacctc gattccaggc
     1801 ctcttcgtct gcggggaggt ttcgggaggt gttgacggcg cgagccgcat gggaggaaac
     1861 gccttgacga atatccttgt cttcggtctc cgtgcgggga aagcggccgc tgaagaggct
     1921 atggcgtctt ccgcggtttc tgacgccgta agcaaattac cggaatgtgt gcttcgatcg
     1981 gggcgggaag gcgtctctcc cgcgaaagtg cgctgcgaac tccgcagtat agtccaggaa
     2041 gggctttctc catggcggaa tgcggaaggg attgagcggt gcatctcgaa gattgaactc
     2101 ctgaacgctt cacgaaaagc gctgaaaatc gcgtcttctc tcgatcttct gcacgcgata
     2161 gaattacacg gtctccatcg taccgcggag tccgtgggaa gggctgcctt cgcccggcag
     2221 gagagccgcg gcgtccattt ccgggatgac tacccgaacg agcgggagga g
//
LOCUS       DAOTIL010000429         2270 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminobacteriaceae bacterium isolate AMR_MDS_3026
            NODE_56429_length_2270_cov_3.649661, whole genome shotgun sequence.
ACCESSION   DAOTIL010000429 DAOTIL010000000
VERSION     DAOTIL010000429.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868050
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminobacteriaceae bacterium (wastewater metagenome)
  ORGANISM  Aminobacteriaceae bacterium
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae.
REFERENCE   1  (bases 1 to 2270)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2270)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:19:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,224
            CDSs (total)                      :: 3,198
            Genes (coding)                    :: 3,184
            CDSs (with protein)               :: 3,184
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2270
                     /organism="Aminobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_56429_length_2270_cov_3.649661"
                     /isolate="AMR_MDS_3026"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:3034609"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..937
                     /locus_tag="P5201_09085"
     CDS             <1..937
                     /locus_tag="P5201_09085"
                     /inference="COORDINATES: protein motif:HMM:NF012993.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="carbon-nitrogen hydrolase family protein"
                     /protein_id="HRV98732.1"
                     /translation="AVIMDKEGTLEKTERLAIEASEKGAKIVLFPEAFIPCYPRGLSF
                     GAVVGSRSPGGRKDFARYYANSIDVPGPETDFLGELAAQTGTYLSIGVIEREGGTLYC
                     TLLYFGPDGALLAKHRKLKPTGSERLIWGEGDGSTLAAVETPYGTMSGLICWENYMPL
                     ARAAVYGRNPSIYLAPTADQRDGWQCSMRHIALEGRCFVLSCNQFVTKEMYPSDLACF
                     SDLEQQPEVMSRGGSAIIDPLGNYVAGPLWDKEGMLVADLDLEAVVEARYDFDVMGHY
                     ARPDVFTLLVDDEPRSGVEFSGDASAWEEDCSCQG"
     gene            925..>2270
                     /locus_tag="P5201_09090"
     CDS             925..>2270
                     /locus_tag="P5201_09090"
                     /inference="COORDINATES: protein motif:HMM:NF012503.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HRV98733.1"
                     /translation="MSGVRNRSEGFGALPRLLDETAKTAGEWLAALPEREVNAATSAE
                     ELREALGGPLNEEGELAADVLRNFAHAAERGLVASPGPRYFGFVTGGALPASLAADWL
                     TSAWDQNAVLFVSSPAASVAEEVVASWLLDLLGFPADASVGLVTGCQMANFTCLAAAR
                     HEVLRKAGWDVEADGLCGAPRITVAAGEEAHATLLVALRMLGLGERNVLPIPADEQGR
                     MIPSALKEALETVSGPLIVCLQAGNVNSGAFDPCAELVPLAKAKGAWVHVDGAFGLWG
                     ALLPELAAHTEGIGLADSWATDAHKWLNVPYDSGIAVVRNEAAHRASMTLKAPYYIAA
                     PGAVRDPSQWVPESSRRARVFPLYCALRSLGKNGLREMIARNCAQARLMASLLAEDPG
                     IRILNDVVLNQALVRFIPPRGGDADAFTRRVTATVQEEGTCWSGGSVWKGMAAMRIS"
BASE COUNT          367 a          713 c          797 g          393 t
ORIGIN      
        1 ggccgttatt atggacaagg aagggactct ggagaaaacc gaaaggctcg cgatcgaagc
       61 ttcggaaaag ggggcgaaga tcgtgctctt tccggaggcg ttcatcccgt gctatccgcg
      121 gggactctcc ttcggcgccg tcgtgggcag ccgttcgccg gggggccgga aggacttcgc
      181 ccggtattac gccaactcca tcgacgttcc cggtccggag accgattttc tgggggagct
      241 ggcggcgcag accgggacgt atctctccat cggagttata gaaagggagg gaggtactct
      301 ctactgcact cttctctact tcggccccga cggtgcgctt ctggccaaac accggaagct
      361 gaagcccacg gggagcgagc ggctcatctg gggagagggc gacggaagca cgctggcggc
      421 ggtggagacg ccctacggga cgatgagcgg cctcatctgc tgggagaact acatgccgct
      481 cgcgcgggca gccgtctacg gccggaaccc ctcgatctac ctcgcgccca cggcggacca
      541 gcgcgacggc tggcagtgct ccatgcgcca catcgctctg gaggggcgct gtttcgtgct
      601 ttcgtgcaac cagttcgtca ccaaggagat gtacccgtcc gacctcgcct gtttttccga
      661 cctcgaacag cagccggagg tcatgagcag aggagggagc gcgattatcg atcctctggg
      721 gaactacgtc gccggtccgc tctgggacaa ggagggaatg ctcgtcgccg acctcgacct
      781 ggaagccgtc gtcgaggcgc ggtacgactt cgacgtgatg ggccactatg cccggcccga
      841 cgtcttcacg ctgctggtcg acgatgagcc gaggagcggg gtggaatttt ccggagacgc
      901 gagcgcttgg gaggaagact gctcatgtca ggggtgagaa accgctcgga gggcttcggg
      961 gctctgcccc gtctgctcga cgagacggcg aagacggcag gagagtggct tgcagccctt
     1021 ccggaaagag aggtcaatgc ggccacgtct gcggaggagc ttcgggaagc tctgggcggg
     1081 ccgctgaacg aggagggcga gctcgccgca gacgtgctgc ggaacttcgc gcacgccgcc
     1141 gaaagaggcc ttgtcgcctc gcccggcccg cgctatttcg gattcgtgac gggcggggcg
     1201 cttcccgcga gcctcgccgc ggactggctg acttccgcgt gggaccagaa cgcggtgctt
     1261 ttcgtctcct ctcctgcggc gagcgtggcc gaggaggtcg tcgcctcctg gctgctggat
     1321 ctgctcggct ttcctgcgga cgcgtcggtc ggcctggtta ccggctgcca gatggcgaac
     1381 ttcacgtgcc ttgccgccgc gcgccacgaa gtcctcagaa aggccggatg ggacgtcgaa
     1441 gcggacggtc tctgcggcgc tccgcgaatc accgtcgccg ccggggagga ggcgcacgcc
     1501 acgctgcttg tcgcgcttcg gatgctcggc ctcggcgagc ggaacgtgct cccgattccc
     1561 gccgacgagc aggggcgaat gatcccgtcc gcgttgaaag aggctctgga aaccgtctcc
     1621 ggtccgctaa tcgtctgcct tcaggcggga aatgtgaact ccggagcctt cgacccgtgc
     1681 gccgagcttg ttcctcttgc gaaggccaag ggggcgtggg tgcatgtgga cggcgcgttc
     1741 ggcctgtggg gcgcgctgct gccggagctt gccgctcaca ccgaaggcat cggactggcg
     1801 gactcctggg cgacggacgc gcacaagtgg ctgaacgtcc cctacgactc ggggattgcc
     1861 gtcgtgcgga acgaggctgc ccacagagcg tcgatgacgc tgaaggcgcc ctactacatc
     1921 gccgcgccgg gggccgtccg cgatccgtcg cagtgggttc ccgagtcctc cagacgggcc
     1981 agggtgttcc cgctgtactg cgcgctccgc tccctcggga agaacggcct ccgggagatg
     2041 atcgcgcgga actgcgccca ggcccgattg atggcgtcgc ttcttgccga agaccccgga
     2101 atccggattc tcaacgacgt cgttctcaac caggcgctcg ttcggttcat ccctccgcgc
     2161 ggcggcgacg cggacgcctt cacgaggcgc gtgactgcca cggtgcagga ggagggaacc
     2221 tgctggtcgg ggggaagcgt ctggaagggg atggccgcca tgcgcatctc
//
LOCUS       DAOTIL010000430         2261 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminobacteriaceae bacterium isolate AMR_MDS_3026
            NODE_56735_length_2261_cov_3.496827, whole genome shotgun sequence.
ACCESSION   DAOTIL010000430 DAOTIL010000000
VERSION     DAOTIL010000430.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868050
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminobacteriaceae bacterium (wastewater metagenome)
  ORGANISM  Aminobacteriaceae bacterium
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae.
REFERENCE   1  (bases 1 to 2261)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2261)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:19:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,224
            CDSs (total)                      :: 3,198
            Genes (coding)                    :: 3,184
            CDSs (with protein)               :: 3,184
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2261
                     /organism="Aminobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_56735_length_2261_cov_3.496827"
                     /isolate="AMR_MDS_3026"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:3034609"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..915
                     /locus_tag="P5201_09095"
     CDS             <1..915
                     /locus_tag="P5201_09095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014163170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HRV98734.1"
                     /translation="KEAVALSYKLIEQDKIAAFVAGSYSLPTRAVAPIFQEEQIPLVA
                     AYAIHPDVTTAGDFCFRNGFLGFVEGKAAGYASHKLLGAKTVALLTSDNDFGRTLVTG
                     FKEYAAKYAPDLKIVSEQAYPFSEKDYKPYLSKIKELNPDVIFASGYYFQTGPLLRQA
                     RELGIESKILGEEGADSPKLVEIAGEAAEGFHIVTNFDRDDPRPVVQNFLKEFRGRHK
                     FEPDMVGASAYDAFMIIVNAMKTAGTTDGKPVRDAIAATVGYDGLTGVMGGFNEIGEV
                     VKPVQVQVVKEGLFRHFGVVDDPELIKP"
     gene            1330..>2261
                     /locus_tag="P5201_09100"
     CDS             1330..>2261
                     /locus_tag="P5201_09100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008709835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004356 - glutamate-ammonia ligase
                     activity [Evidence IEA];
                     GO_process: GO:0006542 - glutamine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine synthetase beta-grasp
                     domain-containing protein"
                     /protein_id="HRV98735.1"
                     /translation="MKLSVLKNFKPGPIEADFLNMLMFDIGGGMRSVSLPRGYVSEAI
                     FRDGIGFDASNYGFAKVDQSDMVAIPDCNAAWLEERGDFSIVHVLCDVVSTERKVFDQ
                     YPRSVAERTAEYLKEKGLADSAQMLVELEYYVFDSVEYSTGLDHAFYSIGSGEGLGED
                     FNTQQRFAPLKGYHKLPPEDRFFDFRNETVRLMEEAGIAVKYHHHEVGASQLEIELDF
                     MDLVKAADSVCIAKWIIHSIAEEMGFKVTFMPKPLYKMPGSGMHVHQFLEKGGKSLFP
                     GGELHGLSKEGLAYTSGLLEHSLTGSLLAFTNPST"
BASE COUNT          475 a          704 c          675 g          407 t
ORIGIN      
        1 aaggaagcgg tcgcgctctc ctacaagctc atcgagcagg ataagatagc ggccttcgtg
       61 gcgggctcgt acagccttcc cacccgcgcc gtggccccca tcttccagga ggaacagatc
      121 cctctggtgg cggcgtacgc cattcatccg gacgtgacca cggccggcga cttctgcttc
      181 cggaacggtt tcctcggctt cgtcgagggg aaggccgccg ggtacgcctc gcacaagctg
      241 ctgggcgcga agacggtcgc cctgctcacc agcgacaacg acttcgggcg gacgctcgtg
      301 accggcttca aggagtacgc cgcgaagtac gctcccgatc tgaagatcgt cagcgagcag
      361 gcctacccct tctcggagaa ggactacaag ccctatctct cgaagatcaa ggagctgaac
      421 cccgacgtta tcttcgccag cggctactac ttccagaccg gcccgctgct tcggcaggcc
      481 cgtgaactcg gcatcgagtc caaaatcctc ggcgaagagg gcgccgactc gcccaagctc
      541 gtcgaaatcg cgggcgaggc cgccgagggc ttccatatcg tcaccaactt cgaccgcgac
      601 gacccccgtc ccgtggtcca gaacttcctg aaggagttcc gcggccgcca caagttcgag
      661 cccgacatgg tcggcgcttc ggcgtacgac gccttcatga tcatcgtcaa cgccatgaag
      721 acggcgggaa cgaccgacgg caagcccgtg cgcgacgcca tcgccgcgac cgtggggtac
      781 gacggcctca ccggcgtcat gggcggcttc aacgagatcg gcgaagtggt caagcccgtg
      841 caggtgcagg tggtcaagga ggggctcttc cgccacttcg gcgtggtcga cgatccggag
      901 ctgatcaagc cgtaagcgga aacgaaacga acgatagata cgctctgcaa aagcaaaagc
      961 ctctccttcg ggggaggctt ttttcatagg ctccgggaaa aaagcgtttt tttcgcgaca
     1021 aaaaatcagc gtttatggta caataaattc cgctcagtga aaaacgacag taaaatatac
     1081 caaacataaa gtatctcaat gtaaagaata cagcctttgc ccgaaaacag cggggatagg
     1141 cttcatacgg gctttcgcgc aacgcctctc cctcggggga ggctttttgc acagtctccc
     1201 ggcgaggggc ctcacgcctt gcgccgacga aattcggggc tgtccggaag cgatttggga
     1261 agcgttttta gttcacggag ggcgcggcgc gttttccgaa caataataca gcgatatgga
     1321 ggaatacgaa tgaaactgtc ggtactcaag aatttcaagc ccggtcccat tgaagcggat
     1381 tttctgaaca tgctgatgtt cgacatcggc ggcggcatgc gctcggtgag tcttccgaga
     1441 gggtacgtct ccgaagcgat cttccgcgac ggcatcggct tcgacgcgtc caactacggc
     1501 ttcgccaagg tcgaccagtc cgacatggtc gccatcccgg actgcaacgc ggcgtggctg
     1561 gaagagcgcg gcgacttcag catcgtccac gtgctctgcg acgtcgtctc caccgagcgg
     1621 aaggtcttcg accagtaccc tcgttccgtc gcggagcgca ccgcggagta cctgaaggag
     1681 aaggggctcg ccgacagcgc gcagatgctc gtcgagcttg agtactacgt cttcgactcc
     1741 gtcgaatact ccacggggct cgaccacgcc ttctactcca tcggttcggg cgaagggctc
     1801 ggcgaggact tcaacacgca gcagcgtttc gccccgctga aggggtacca caagctgccg
     1861 cccgaggacc gcttcttcga cttccggaac gagaccgtcc ggctgatgga agaggcgggc
     1921 atcgcggtga agtaccacca ccacgaggtg ggcgcgtcgc agctcgagat cgagctggac
     1981 ttcatggatc tggtgaaggc cgccgacagc gtctgcatcg ccaagtggat catccactcg
     2041 atcgccgagg agatgggctt caaggtgacc ttcatgccga agccgctcta caagatgccc
     2101 ggcagcggca tgcacgtcca ccagttcctc gaaaagggcg ggaagagcct ctttccgggc
     2161 ggcgaactcc acggtctgtc gaaggagggg cttgcctaca cctccggcct gctggagcac
     2221 agtctcactg ggagcctgct cgccttcacg aaccccagca c
//
LOCUS       DAOTIL010000431         2260 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminobacteriaceae bacterium isolate AMR_MDS_3026
            NODE_56766_length_2260_cov_3.124717, whole genome shotgun sequence.
ACCESSION   DAOTIL010000431 DAOTIL010000000
VERSION     DAOTIL010000431.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868050
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminobacteriaceae bacterium (wastewater metagenome)
  ORGANISM  Aminobacteriaceae bacterium
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae.
REFERENCE   1  (bases 1 to 2260)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2260)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:19:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,224
            CDSs (total)                      :: 3,198
            Genes (coding)                    :: 3,184
            CDSs (with protein)               :: 3,184
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2260
                     /organism="Aminobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_56766_length_2260_cov_3.124717"
                     /isolate="AMR_MDS_3026"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:3034609"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..187
                     /locus_tag="P5201_09105"
     CDS             <1..187
                     /locus_tag="P5201_09105"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRV98736.1"
                     /translation="GFRRPERGALLSGFYFVHFLTVCLEALARLIQGKGKPFPDERSA
                     DCGGSRARISSAVDDTA"
     gene            211..1557
                     /locus_tag="P5201_09110"
     CDS             211..1557
                     /locus_tag="P5201_09110"
                     /inference="COORDINATES: protein motif:HMM:TIGR04564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SYNERG-CTERM sorting domain-containing protein"
                     /protein_id="HRV98737.1"
                     /translation="MKRMKACCLAIAAAITLCLAGFAPRAEATIWKVTRTDDNPSNPA
                     EGSLRHAVNNAWNNDFITFESAGMTIDLSEELSLSKTLTISGSGTVSPTTIRQTAPGK
                     RVFSIAPGVFGPRFEYLIIRGGNAAESGGAILNRGYSLTLHRCTLDGNSAANYGGGID
                     NHGKLTLNIVTVKNNSASSGGGIKNRTGSSCDMYMSTITGNTPDQIVGAYTTDGHCTI
                     GDSAGTASVAFGGIPEGLSPKPRKTSGEADVTAVENDLKKPESSLFAAVKNALSADLG
                     GLPGDVSAGLSGMNATLYDAFAYEDIPLSDTSGEGKLEVEFTASWPKNVRYYAALAEY
                     EDAAAGARAVKGYVIPERGVQFEIKPGQSLPGGMEPPDFYVEGEGLMTWRNVVEDNGP
                     FDHNRAAGMVTFRAVSIRAEAQTAPTGGSGCSSATASPFALLLGVPLLLLRKTRRI"
     gene            1776..>2260
                     /locus_tag="P5201_09115"
     CDS             1776..>2260
                     /locus_tag="P5201_09115"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRV98738.1"
                     /translation="MVQRGCFGFRALRMITVMVLLLCPAPQAAAVAMWNVTSGADDPS
                     NPAVGTFRYTVLHASPGDTVRFTGAGTTVVLADQVLIDRTLTLNGPATIRQGAVNKRV
                     LEVTATCTMNNITITGGNFENQGLSALKGAGIFNRTAGVLTMNSCTVTGNTRGGAIEN
                     YG"
BASE COUNT          462 a          696 c          730 g          372 t
ORIGIN      
        1 cggttttcgc aggccggagc ggggagcgct tctctccggc ttttactttg tacattttct
       61 tacggtctgt cttgaggccc ttgcgcgcct cattcagggg aagggaaagc cgtttccgga
      121 cgaacgatcc gcagactgcg ggggcagccg cgctagaatc tcctccgccg ttgatgatac
      181 tgcataaaca gcaaaaggag gtcgaacttg atgaaaagga tgaaagcctg ctgtcttgcg
      241 atagcggcgg cgattacgct ctgtctggcg ggattcgccc cgcgggcgga agcgacgata
      301 tggaaggtga cgaggacgga cgacaatccg tcgaacccgg cggaggggtc gctgcggcac
      361 gccgtgaaca atgcgtggaa caacgacttc atcacgttcg aatcggcggg gatgacgatc
      421 gacctctcgg aggaactctc cctctcgaag acgctgacca tctcgggctc gggaacagtc
      481 agcccgacga caatccggca gaccgccccg ggcaagcgag tcttctcgat cgcgccgggc
      541 gtcttcggcc cccggttcga atacctgatc atcaggggag gcaacgctgc ggagagcggc
      601 ggcgccatcc tcaacagagg gtacagcctg acgctccacc gctgcacgct cgacggaaac
      661 tccgcggcca actacggagg cggcatcgac aaccacggga agctgacgct gaacatcgtc
      721 accgtcaaaa acaacagcgc gtcttcgggc ggcgggatca agaaccggac gggaagctcc
      781 tgcgacatgt acatgtcgac gatcacggga aacacgcccg accagatcgt cggcgcctac
      841 accacggacg ggcactgcac catcggcgac tcggcgggaa cggcctctgt cgctttcggc
      901 ggtatccccg agggcctttc tcccaaaccc cgcaagacca gcggcgaagc cgacgtaacg
      961 gcagtggaga acgacctgaa gaaaccggaa agctcgctct tcgcggcggt gaagaacgcc
     1021 ctctcggcgg acctgggagg gcttcccggc gacgtttcag cggggctttc gggaatgaac
     1081 gcgacgttgt acgacgcctt cgcctacgag gacataccgc tctccgacac atccggagag
     1141 gggaagctcg aggtggagtt cacggcctcg tggccgaaga acgtccgcta ctacgccgcg
     1201 ctcgcggagt atgaggacgc cgctgcggga gcccgcgccg tgaaggggta cgtcatcccc
     1261 gagcgcggcg tgcagttcga gatcaaaccg gggcaaagcc ttcccggcgg catggagcct
     1321 cccgacttct acgtggaagg cgaagggctg atgacgtggc ggaacgtcgt ggaggacaac
     1381 ggacctttcg accacaaccg cgctgcgggc atggtgacgt tccgcgcggt ctccatccgc
     1441 gccgaggccc agacggctcc cacaggcggc tccggctgct cgtcagcgac cgcgtcgccc
     1501 ttcgcgctct tgttgggagt cccgctgctc cttctgcgga aaacacggcg aatctgaagg
     1561 cgcgctataa ccgacgctcg ccggaagcga ccgacggcac gggaagcgcc gccgcgagag
     1621 aattctccgg cgccatccgc gcctcctaga aggcggaaac gcgtacaacc agccttccgc
     1681 ttccttatgt ctataaacga attcaaattt gctttacttt acgacgcata cttttttcgc
     1741 agaacgcgaa tactcaacga gaggaagcga agactatggt gcaacgaggt tgtttcggtt
     1801 ttcgggcttt gaggatgata acggtgatgg tattgctgct ctgccccgcg ccgcaggcgg
     1861 cggccgtcgc gatgtggaac gtgacgagcg gggcggacga cccgtcgaat cccgctgtgg
     1921 gaaccttccg gtacacggtg ctccacgctt cgccggggga caccgtccgg tttaccggcg
     1981 caggaacgac ggtcgttctg gcggaccagg tgctcatcga caggacgctc acgctcaacg
     2041 gccccgcgac gatccggcag ggagctgtga acaagcgcgt cctcgaagtg acggcgacgt
     2101 gcacgatgaa caacatcacg atcacgggag gcaacttcga gaaccagggg ttgtcggcgc
     2161 tgaagggggc cgggatcttc aatcgcacgg cgggggtcct gacgatgaac tcgtgcacgg
     2221 tgacgggcaa cacgcgcggc ggcgccatcg agaactacgg
//
LOCUS       DAOTIL010000432         2257 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminobacteriaceae bacterium isolate AMR_MDS_3026
            NODE_56894_length_2257_cov_3.313806, whole genome shotgun sequence.
ACCESSION   DAOTIL010000432 DAOTIL010000000
VERSION     DAOTIL010000432.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868050
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminobacteriaceae bacterium (wastewater metagenome)
  ORGANISM  Aminobacteriaceae bacterium
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae.
REFERENCE   1  (bases 1 to 2257)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2257)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:19:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,224
            CDSs (total)                      :: 3,198
            Genes (coding)                    :: 3,184
            CDSs (with protein)               :: 3,184
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2257
                     /organism="Aminobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_56894_length_2257_cov_3.313806"
                     /isolate="AMR_MDS_3026"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:3034609"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..537
                     /gene="otsB"
                     /locus_tag="P5201_09120"
     CDS             <1..537
                     /gene="otsB"
                     /locus_tag="P5201_09120"
                     /EC_number="3.1.3.12"
                     /inference="COORDINATES: protein motif:HMM:TIGR00685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004805 - trehalose-phosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0005992 - trehalose biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trehalose-phosphatase"
                     /protein_id="HRV98739.1"
                     /translation="HGFDITGPDGLKFENTVGEDFLPALDSAENALSEKIGAIEGLFL
                     ERKKFAIAIHYRRVAPENIERIEAAVDETTSDHPDLRKAYGKKIFELQPKADWHKGKA
                     LFSLMRTLELDRDDVLLVFIGDDATDEDAFRALQGRGVGIVVREKAYETAAAYSLKDP
                     DEVREFLLRLIPLCRGSS"
     gene            complement(700..1737)
                     /locus_tag="P5201_09125"
     CDS             complement(700..1737)
                     /locus_tag="P5201_09125"
                     /inference="COORDINATES: protein motif:HMM:NF012335.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding dehydrogenase"
                     /protein_id="HRV98740.1"
                     /translation="MRAVVKYKREDGNTEVRDVPVPEIEASDVLVKVAYIGICGSDPH
                     MYHNQVSYPMAIPLILGHEFSGTIEKVGTDVKKWKVGDRVTAETHARFCGKCVLCKTN
                     NYRFCRERKGYGFGVDGAFAEYVRVPERILHAVPDSVTLRDASCTEPVCVAYNVVIAK
                     TGVKAGDCVAVLGPGPIGLLCAHMAKLAGASHIAVFGAPGDEQRLVIAKQYGATETHL
                     LDGSSDPKSVAAKFNEGYGFNSVIDAAGPAATLKLSMELVMPAGIINKVAWGPKPVDF
                     SLDPLLSKGVTLQGSFSHSWDIWEKVLVIMGQGQMPLDALITHELPLEGWKEGFDLIE
                     SRKGLKVVLKP"
     gene            complement(1787..>2257)
                     /locus_tag="P5201_09130"
     CDS             complement(1787..>2257)
                     /locus_tag="P5201_09130"
                     /inference="COORDINATES: protein motif:HMM:NF014073.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter permease"
                     /protein_id="HRV98741.1"
                     /translation="LQNGHHRFAMLILGSFAMLFWGLALAKPMVAILKIKREILLPIV
                     IPLTVIGAYAGGVRIFDVYLMLAFGVLGYILRQMDYPLAPLVLGLILGPLADTSFRRA
                     LQQSRGAIMPLLERPIGLLLLLCIAWLIWTGIKRTRQYYAEQTAREDGEELSAE"
BASE COUNT          431 a          676 c          705 g          445 t
ORIGIN      
        1 cacggtttcg acatcacggg ccccgacgga ctaaagttcg aaaatactgt aggggaggat
       61 tttctccccg cgctcgacag cgcggagaac gccctgtcgg agaagatcgg cgccattgaa
      121 gggctttttt tggagagaaa aaagttcgcc atcgccatcc actaccggcg ggttgctcca
      181 gaaaacatcg agcggataga ggccgccgtc gacgaaacga cgtcggacca ccccgatctc
      241 aggaaagcct acggcaagaa gatattcgag ctccagccga aggcggactg gcacaagggg
      301 aaggcgctct tctcgctgat gcgtacgctg gaactcgacc gggacgacgt gctccttgtc
      361 tttatcggag acgacgcgac cgacgaggac gcgttccgcg cgttgcaggg aagaggcgtc
      421 ggcatcgtgg tccgggaaaa agcctacgag accgccgctg catacagcct gaaggatccc
      481 gatgaagtcc gggagtttct tctccgtttg attccgttgt gcagggggtc gtcatgatgg
      541 cgtgggatgt gctcgcacag aagagccatt gtcttcgggg ccttgttcgg gaaatcgtcg
      601 gaaacacgga acagcgatat cctgcgaatg gactctcttg aattttgaaa gggaggacgt
      661 gcgaacgccc tccctccaaa aaaatagctt tttcagccgc tacggcttga gcacgacctt
      721 cagtcctttg cggctttcga tgaggtcgaa tccctctttc cacccctcga gcgggagctc
      781 gtgggtgatg agggcgtcga gcggcatctg cccctgtccc atgatgacga gcaccttctc
      841 ccagatgtcc cacgagtggc tgaaggaccc ctggagtgtc acgcccttcg agagcagcgg
      901 gtcgaggctg aaatccacgg gtttcggccc ccatgccacc ttgttgatga ttcctgcggg
      961 cataaccagc tccatcgaga gcttcagcgt cgcggcgggt cctgccgcgt cgatgacgga
     1021 gttgaagccg tacccctcgt tgaacttcgc ggccacgctc ttcgggtccg aggatccgtc
     1081 gaggaggtgc gtctcggtcg cgccgtactg tttcgcaatg acaaggcgct gttcgtcgcc
     1141 cggcgcgccg aagacggcga tgtggctcgc cccggcgagt ttcgccatgt gagcgcagag
     1201 tagccctatc ggtccgggac cgaggacggc gacgcagtcg cccgccttga cgcccgtctt
     1261 cgcgatcacc acgttgtacg ccacgcagac tggctccgtg catgaggcgt cccgcagcgt
     1321 aaccgaatcg ggaacggcgt ggagaatacg ctccggcacg cggacatact ccgcgaacgc
     1381 gccgtcgacg ccgaaaccgt accctttgcg ctcgcggcag aagcggtagt tgttcgtctt
     1441 gcagaggacg cacttgccgc agaagcgggc gtgcgtctcc gcagtcaccc ggtcgccgac
     1501 cttccatttt ttcacgtcgg tcccgacctt ttcgatggtc cccgagaact cgtgtccgag
     1561 gatgaggggg atcgccatgg ggtaggagac ctggttgtgg tacatgtgcg ggtcgcttcc
     1621 gcagatgccg atatacgcga cctttacgag tacgtccgac gcttcgatct ccggtaccgg
     1681 gacgtcccgt acttccgtgt tgccgtcttc tctcttgtat ttcaccactg cgcgcatagt
     1741 ccgttctccc tcctcgccgc cgggaacatc ggcggcgttt cgttgtctat tccgcggaga
     1801 gctcctctcc gtcctctctt gcggtctgtt cggcatagta ctgtcgtgtt cgtttgatcc
     1861 ccgtccagat gagccacgcg atgcacaaca aaagcagcag cccgatgggg cgttcgagca
     1921 gcggcatgat ggcgccgcgg ctctgttgga gcgcccggcg gaagctggtg tcggccagcg
     1981 gaccgaggat gagtccaagg acgagcggcg ccagcggata gtccatctgc cgcaggatgt
     2041 accccagaac gccgaaggcg agcatcaggt agacgtcgaa gatgcgaacg cctcccgcgt
     2101 aggccccgat gacggtcagg gggatgacga tggggagcag gatctcccgc ttgatcttca
     2161 aaatggccac catcggtttg gcgagcgcga gcccccagaa gagcatggcg aacgagccga
     2221 ggataagcat ggcaaaccga tggtggccat tttgaag
//
LOCUS       DAOTIL010000433         2254 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminobacteriaceae bacterium isolate AMR_MDS_3026
            NODE_56993_length_2254_cov_2.921783, whole genome shotgun sequence.
ACCESSION   DAOTIL010000433 DAOTIL010000000
VERSION     DAOTIL010000433.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868050
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminobacteriaceae bacterium (wastewater metagenome)
  ORGANISM  Aminobacteriaceae bacterium
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae.
REFERENCE   1  (bases 1 to 2254)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2254)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:19:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,224
            CDSs (total)                      :: 3,198
            Genes (coding)                    :: 3,184
            CDSs (with protein)               :: 3,184
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2254
                     /organism="Aminobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_56993_length_2254_cov_2.921783"
                     /isolate="AMR_MDS_3026"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:3034609"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..229
                     /locus_tag="P5201_09135"
     CDS             <1..229
                     /locus_tag="P5201_09135"
                     /inference="COORDINATES: protein motif:HMM:NF018513.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="TRAP transporter large permease subunit"
                     /protein_id="HRV98742.1"
                     /translation="GMMICINMQMAFQTPPMAMSIFVLKGTAPKELGVTIGDIIWGVI
                     PFILIIMFVLLLCAIFPGIITWLPELMIGKA"
     gene            complement(319..1863)
                     /locus_tag="P5201_09140"
     CDS             complement(319..1863)
                     /locus_tag="P5201_09140"
                     /inference="COORDINATES: protein motif:HMM:NF014764.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose--hexose-1-phosphate
                     uridylyltransferase"
                     /protein_id="HRV98743.1"
                     /translation="METAVAVRAVDSLIEYGRRRRLLGELDDIVARNSLLGLLGIEEP
                     DPTERSGESMDEMASIETLRLYAKERGIAHLDLPAEKFVSRIMGFLTPRNSEVVREFR
                     RLMEQEGAAAAVAWFYRFSADTMYVRMDLVSLDRKWTAMTEFGEMQITINRSKPEKDP
                     RDIRAAGAQTGEDRYPKCLLCAENAGYQGRPGHPERSNHRIVPLELSGEQWYMQFSPY
                     VYYHHHCIVFSKEHRPMRIDRMTFVRICDFLTLFPGMFIGSNADLPIVGGSILSHDHF
                     QGGTWTFPMTNAPVLESLRVAGASLEHLRWPLTTLRLRSPDRTVLETLGEQLLLSWRE
                     YEDRERGILARSGNGEREEQHNTVTVIGRRRGAEYEIDVVLRNNRCDEQHPDGIFHAH
                     RERHHIKKENIGLIEVMGLAILPPRLESSLNDVAAILSGEKTLSDLPQAFPHTEWTVE
                     LATRFGTTLPADEAERIVRNEVGTVFSKVLRDCGVFKATEDGEKGVERFLSSWAAQWG
                     RSFASD"
     gene            complement(1870..>2254)
                     /locus_tag="P5201_09145"
     CDS             complement(1870..>2254)
                     /locus_tag="P5201_09145"
                     /EC_number="4.2.1.47"
                     /inference="COORDINATES: protein motif:HMM:NF027681.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="GDP-mannose 4,6-dehydratase"
                     /protein_id="HRV98744.1"
                     /translation="GRRESISIFGDDYDTPDGTCIRDYVHVTDLAQAHLLTLDALRRG
                     EGNKTFNLGNGRGFSVHEVIDACRRVTGREIRVTLSPRRPGDPARLIASSERIQRELR
                     WKPRYTDLDAIVETAWRWHLKYRKD"
BASE COUNT          405 a          669 c          681 g          499 t
ORIGIN      
        1 cggcatgatg atctgcatca acatgcagat ggcattccag acgccgccga tggcgatgtc
       61 gatcttcgtc ctcaagggga ccgcgcccaa ggagctcggt gtgacgatcg gcgacatcat
      121 ttggggcgtc attccgttca ttctcatcat catgttcgtt ctcctgctct gcgccatctt
      181 cccggggatc atcacctggc tccccgaact gatgatcggc aaggcgtagg gacccctcag
      241 tcataccgaa acacaaacgg cggcccgctt ttttcggggt cgccgttctt tttggagaca
      301 cactccttgg gggcctcttc agtctgatgc aaacgaacgc ccccactgcg ccgcccagga
      361 ggagagaaat cgttcgactc ctttttctcc gtcctcagtt gccttgaaca ctccgcaatc
      421 cctgaggact ttggaaaaaa ctgtgccgac ctcgttccgg acgatacgct ctgcttcgtc
      481 tgcaggaaga gtggtaccga aacgggttgc cagttcaact gtccattcgg tgtggggaaa
      541 ggcctgcgga agatccgaaa gagttttttc tcctgagaga atcgcggcca cgtcgttcag
      601 gcttgattcc agacggggag gaagaatcgc gagccccata acttcgatga gtccgatatt
      661 ctcttttttg atatgatggc gttccctgtg cgcgtggaat atgccgtcgg ggtgttgctc
      721 gtcgcaccgg ttgttccgca gcacgacgtc tatttcgtac tctgcgcccc gccgtctgcc
      781 gatgaccgtg acggtgttgt gctgttcctc tctttctccg tttccgcttc gggcgagtat
      841 tcccctctct ctgtcctcgt actcgcgcca ggagaggagc agctgctcgc cgagcgtttc
      901 caggacggtt ctgtcggggc ttctcaggcg aagagttgtc aagggccagc ggagatgttc
      961 cagggatgcg ccggcgacac gcagactctc cagaacaggc gcgttcgtca tcggaaaagt
     1021 ccacgtacct ccctgaaagt gatcgtgact gagaatagaa cctccgacga tggggaggtc
     1081 ggcattcgat ccgatgaaca tgccggggaa aagcgtcaga aaatcgcaga tgcggacaaa
     1141 cgtcatcctg tcgatgcgca tcggccggtg ttccttcgag aagacgatac agtggtgatg
     1201 gtagtagacg tagggggaga actgcatgta ccattgttcg cccgacagtt cgagcgggac
     1261 gatacgatgg ttgctccgct cggggtgtcc ggggcgtccc tggtagcccg cattctcggc
     1321 gcagagcaga catttcggat aacgatcctc gcccgtttgt gctcctgcgg cgcggatatc
     1381 tcttggatct ttctcaggct tggaccgatt gatggtgatc tgcatctcgc cgaactccgt
     1441 catcgccgtc cacttccggt ccagcgagac aagatccatc ctgacgtaca tcgtgtcggc
     1501 ggagaagcgg tagaaccagg cgacggccgc cgcggccccc tcctgctcca tgaggcggcg
     1561 gaattcacgg acgacctccg aattgcgggg agtcaggaac cccatgatac ggctgacaaa
     1621 cttctcggcg ggaaggtcaa gatgcgcaat gccgcgctct ttcgcgtaga ggcgcagcgt
     1681 ttcgatgctc gccatctcgt ccatgctctc ccctgatctt tccgtagggt cgggttcttc
     1741 tatgccgaga agcccgagga gggagttccg tgcgacaata tcgtccagct cgcccaagag
     1801 gcggcgccgc cgcccgtact cgatgagact gtcgacggcg cggacggcga cagcggtctc
     1861 cacgtcggct cagtcctttc tgtatttcag gtgccaccgc cacgcggtct cgacgatggc
     1921 atccagatcg gtgtagcgcg gcttccagcg gagttccctc tggatacgct ccgaggaggc
     1981 gatgagacgg gcggggtcgc ccggtctcct cggggagagc gtcacccgga tctcccgtcc
     2041 ggtgacgcgg cggcaggcgt cgatgacttc gtgtacggag aagccgcggc cgttgccgag
     2101 attgaaggtc ttgttcccct cgccccttct gagcgcgtcg agggtcagaa gatgcgcctg
     2161 cgcgaggtcg gtcacgtgga cgtagtccct gatgcaggtc ccgtcggggg tgtcgtagtc
     2221 gtctccgaag atggagatgc tctcccgtct gccc
//
LOCUS       DAOTIL010000434         2253 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminobacteriaceae bacterium isolate AMR_MDS_3026
            NODE_57031_length_2253_cov_3.018198, whole genome shotgun sequence.
ACCESSION   DAOTIL010000434 DAOTIL010000000
VERSION     DAOTIL010000434.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868050
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminobacteriaceae bacterium (wastewater metagenome)
  ORGANISM  Aminobacteriaceae bacterium
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae.
REFERENCE   1  (bases 1 to 2253)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2253)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:19:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,224
            CDSs (total)                      :: 3,198
            Genes (coding)                    :: 3,184
            CDSs (with protein)               :: 3,184
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2253
                     /organism="Aminobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_57031_length_2253_cov_3.018198"
                     /isolate="AMR_MDS_3026"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:3034609"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(245..469)
                     /locus_tag="P5201_09150"
     CDS             complement(245..469)
                     /locus_tag="P5201_09150"
                     /inference="COORDINATES: protein motif:HMM:NF019540.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicA family
                     toxin"
                     /protein_id="HRV98745.1"
                     /translation="MTKLPVVTGKALIAALEDIGFKVLRIKGSHHYLRHVDGRSTVVP
                     VHAGEEVGPGLLVKILRDCELSKETLENLL"
     gene            complement(466..681)
                     /locus_tag="P5201_09155"
     CDS             complement(466..681)
                     /locus_tag="P5201_09155"
                     /inference="COORDINATES: protein motif:HMM:NF027249.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicB family
                     antitoxin"
                     /protein_id="HRV98746.1"
                     /translation="MARQFSVIIEQDQDGYYVASVPALRGCHTQAKSLDELSVRVKEA
                     IELCLEVEDNDAERLEFIGVQQVSVPI"
     gene            701..922
                     /locus_tag="P5201_09160"
     CDS             701..922
                     /locus_tag="P5201_09160"
                     /inference="COORDINATES: protein motif:HMM:NF018476.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA];
                     GO_process: GO:0006401 - RNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system YoeB family
                     toxin"
                     /protein_id="HRV98747.1"
                     /translation="MILTDGQPCRNGIPLKHAPVGFWSRRITGEHRIVYRVEDDALLI
                     AQLRLTRPLPAIVRKSFLKRDVGSTSYIP"
     gene            complement(902..1144)
                     /locus_tag="P5201_09165"
     CDS             complement(902..1144)
                     /locus_tag="P5201_09165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006301515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRV98748.1"
                     /translation="MSRATLEPTKRKELYRRFRNLSEGNAARVLGSVDALEEERPGDE
                     TLAALREADAIARDPSVRGFTDVVEMMESILRDVRS"
     gene            complement(1324..1587)
                     /locus_tag="P5201_09170"
     CDS             complement(1324..1587)
                     /locus_tag="P5201_09170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008711124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008657 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) inhibitor activity
                     [Evidence IEA];
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA];
                     GO_process: GO:0030541 - plasmid partitioning [Evidence
                     IEA];
                     GO_process: GO:0051290 - protein heterotetramerization
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HRV98749.1"
                     /translation="MGWRIELSRRAEKQPAAIDATEALRIVRYLSELEKLESPRSRGK
                     GLTADLSGLWRYRVGDWRVLCRIEDGRLLVLVVEIGHRSTVYN"
     gene            complement(1571..1795)
                     /locus_tag="P5201_09175"
     CDS             complement(1571..1795)
                     /locus_tag="P5201_09175"
                     /inference="COORDINATES: protein motif:HMM:NF017333.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribbon-helix-helix protein, CopG family"
                     /protein_id="HRV98750.1"
                     /translation="MLALRLDEKLSSRLDALARKTGRTKTWYARKAIEAYIDDMEDAA
                     LAAAAYEEYLLDGGASSPLNEVRRRLGLED"
     gene            2008..>2253
                     /locus_tag="P5201_09180"
     CDS             2008..>2253
                     /locus_tag="P5201_09180"
                     /inference="COORDINATES: protein motif:HMM:NF014643.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008219 - cell death [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system
                     prevent-host-death family antitoxin"
                     /protein_id="HRV98751.1"
                     /translation="MNTITYTAARANLADTMDKVCNDHEAIIITRKSDQAVVMISLEE
                     YNALEETAHLLRTPANAGRLLSAVAQLRAGKGMERKLH"
BASE COUNT          510 a          645 c          593 g          505 t
ORIGIN      
        1 cgagaaaccc gatcttcgtt ttaccatagt ccatctactt cggcaagact tttccaggat
       61 tattttcgaa aagagcaata aatttccatg ctactacttc gacagggagg catagttcgt
      121 ttggggagaa tgagcaaggg ggtatatgga ggcgactcga atcgccgccg aagggccgat
      181 gtgcagcgca tcaagcgcag tgagtattcg ccggaagtgc gttatggagg aaaacatcaa
      241 tagctcaaag cagattttcc aaagtctcct tggagagttc acaatcccgt agaatcttca
      301 ccagaagccc ggggccgacc tcttctcccg cgtgaacagg gacgacggta gaacggccgt
      361 cgacatgccg caggtaatga tggctgccct tgattcgcag gaccttgaat ccgatatctt
      421 caagtgcggc tatcaacgct ttccccgtca cgacaggaag ctttgtcata ttggaacgct
      481 cacctgctgg actccgatga attcgaggcg ctccgcatcg ttgtcctcca cctcaagaca
      541 cagttcgatg gcctctttta cccgtacgga aagttcgtcg agcgacttcg cctgagtatg
      601 acaccctcgc agtgcaggta ctgaagcgac gtaatagcca tcctgatcct gttcaatgat
      661 aacgctgaac tgcctggcca tgatccccac ctccccgagt atgattctaa cagatggaca
      721 gccctgtaga aacggaatcc ccctgaagca tgctccggtc ggtttttggt cgcgccggat
      781 aaccggagaa catcgtatcg tctatcgcgt cgaggacgat gcgcttttga tcgcgcagct
      841 tcgattaacg cgcccgcttc cggcgatcgt ccgcaaatct ttcttgaagc gcgacgtcgg
      901 ctcaacttcg tacatccctt aaaattgatt ccatcatttc gacgacgtcg gtaaatcctc
      961 tcaccgaagg atcgcgggcg attgcatcgg cttcccgcag cgcggcgagc gtttcatcgc
     1021 cggggcgctc ttcttccagg gcgtcgacgg agcccagcac acgcgcggca ttcccttccg
     1081 agagattgcg gaagcgtcgg tacagctctt ttcttttcgt cggttcaagc gtagcccgag
     1141 acattgttcc cctcccgatg cgctcctgtg cgcacccgta ttgtatctca tgccgtacat
     1201 gccgttcaac agaaggaacg cggacttttc caagattatt ttcgaaaaga gcgataaatt
     1261 cccatgcgta ctactcgctc ttctccgtca ggaggttttc atagttcgtc gatcgcggtg
     1321 gggctagttg tacaccgtgc ttctatgccc aatctccact accaacacca gaagcctgcc
     1381 gtcttcgata cggcacagga cgcgccaatc ccccacgcga tatcgccaga gaccggacag
     1441 atcggctgtc agtcccttgc cgcgagaacg cgggctttca agtttttcca gctcggacag
     1501 gtatcgtacg atccgcagag cttcggtagc atcgatggcg gccggctgct tctcggcacg
     1561 gcgggagagt tcaatcctcc agcccaagac ggcgcctcac ttcattcaga ggagaagagg
     1621 cgcctccgtc gagaaggtac tcttcgtaag cggccgcagc aagagcggcg tcctccatat
     1681 cgtcgatgta tgcctcgatg gctttgcggg cataccatgt cttggtccgg cccgtctttc
     1741 gcgccaaggc gtcaaggcgc gatgagagtt tttcgtccag tcgcagagca agcatagaaa
     1801 ctcctccttt tcatgctata caagtatagc atgaaaaacc gctccaattc cggagctgct
     1861 atagcgttga gtgtgcttca tggaagcgat gtccgctgat ttctgctcac ggggcaactc
     1921 tccgagacct tacgatccag cgaacaagcg cttgacgcat cgcttttcaa gatgtacagt
     1981 aagatgtaca gaaaaggagg gaatcccatg aacacgataa cctatacagc ggcgcgggcg
     2041 aatcttgcgg atacgatgga taaggtctgc aacgatcatg aagcgatcat catcacgcga
     2101 aagagcgatc aggccgtcgt tatgatctcg ctggaggagt acaacgccct tgaagaaacg
     2161 gcgcacctgc tgcggacacc cgccaacgca gggaggctgc tctcggccgt cgctcagctg
     2221 agggccggta agggcatgga gcggaagcta cac
//
LOCUS       DAOTIL010000435         2252 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminobacteriaceae bacterium isolate AMR_MDS_3026
            NODE_57055_length_2252_cov_3.640419, whole genome shotgun sequence.
ACCESSION   DAOTIL010000435 DAOTIL010000000
VERSION     DAOTIL010000435.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868050
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminobacteriaceae bacterium (wastewater metagenome)
  ORGANISM  Aminobacteriaceae bacterium
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae.
REFERENCE   1  (bases 1 to 2252)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2252)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:19:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,224
            CDSs (total)                      :: 3,198
            Genes (coding)                    :: 3,184
            CDSs (with protein)               :: 3,184
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2252
                     /organism="Aminobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_57055_length_2252_cov_3.640419"
                     /isolate="AMR_MDS_3026"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:3034609"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..180)
                     /locus_tag="P5201_09185"
     CDS             complement(<1..180)
                     /locus_tag="P5201_09185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008708904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-binding protein"
                     /protein_id="HRV98752.1"
                     /translation="MNGAQMVVKALELEGVRCVFGIPGGSVIPLYDALYDAPFPHILV
                     RHEQAPLARNEAEELV"
     gene            complement(222..1232)
                     /gene="ilvC"
                     /locus_tag="P5201_09190"
     CDS             complement(222..1232)
                     /gene="ilvC"
                     /locus_tag="P5201_09190"
                     /EC_number="1.1.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014806060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketol-acid reductoisomerase"
                     /protein_id="HRV98753.1"
                     /translation="MAKALYDKDANMKLLEGKTVAVLGYGSQGHAHSQNLRDSGVSVV
                     VGLHEGSKSKATAEKDGFEVYTVAEATKRADVVMFLMPDHVQGDTYRKHVAPNLKKGA
                     ALAFAHGFSVHFNQVEPSKDNDVFMVAPKGPGHLVRRMYTEGRGVPCLLAVQQDASGQ
                     ARDLGLAYASALGGGRAGVLSTTFKEETETDLFGEQAVLCGGITELIKAGFDTLVEAG
                     YQPEIAYFECLNEMKLIVDLMYKGGMGWMRFSVSDTAKYGDLTAGKKVVDGHVRQTMK
                     ELLANVQDGSFAKDWILENQCGRPRMKKWMEQEKAHPIEAVGKELRAMMPWMDAKSAP
                     GE"
     gene            complement(1264..1755)
                     /gene="ilvN"
                     /locus_tag="P5201_09195"
     CDS             complement(1264..1755)
                     /gene="ilvN"
                     /locus_tag="P5201_09195"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: protein motif:HMM:NF008864.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:1990610 - acetolactate synthase regulator
                     activity [Evidence IEA];
                     GO_process: GO:0009082 - branched-chain amino acid
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase small subunit"
                     /protein_id="HRV98754.1"
                     /translation="MERTIGVVAENRPGVFSRISGLMERRGFQVKGVTAGVTQQPGRS
                     RFTLVIAGDDRKASQAVRQIRKMVETVEVSDLSSGFCVERCMMLMKFSLPAERRAELF
                     DAMNAFPHTVASEHGDTVILETTGTGENLDACLETVRHLGLVEAVKSGPLALDARTTT
                     TTF"
     gene            complement(1892..2101)
                     /locus_tag="P5201_09200"
     CDS             complement(1892..2101)
                     /locus_tag="P5201_09200"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRV98755.1"
                     /translation="MPAIRTARNERPSRRTPWYHTIGKDDIEAKEETPVKNMSTTTHS
                     ALTFVVAGAIIIIRCGDSPGALLVV"
BASE COUNT          382 a          700 c          682 g          488 t
ORIGIN      
        1 tacaagctct tcggcctcat tccgggcgag cggcgcctgc tcgtgccgga cgaggatgtg
       61 cgggaaggga gcgtcgtaga gtgcgtcgta cagagggatc accgaacctc cggggattcc
      121 gaacacacac cggactccct ccagttcaag ggcttttacc accatctgcg ctccgttcat
      181 cttcatgctc ctctctccct tcccatctga aataaactgg tttattcgcc cggtgccgac
      241 ttggcgtcca tccagggcat catcgcgcga agctctttgc cgaccgcctc gatcgggtgc
      301 gccttctcct gctccatcca cttcttcatg cgggggcgtc cgcactggtt ctccaaaatc
      361 cagtcctttg cgaacgaacc gtcctgcacg ttggcaagga gttccttcat cgtctgccgg
      421 acgtgcccgt cgacgacctt ctttccggcg gtgaggtcgc cgtacttcgc cgtgtcgctg
      481 acggagaaac gcatccaccc catgccgccc ttgtacataa ggtcgacgat cagcttcatc
      541 tcgttgaggc actcgaagta ggcgatctcc ggctggtatc cggcctcgac gagggtgtcg
      601 aatcccgcct tgatcagctc ggtgatgccg ccgcagagga cggcctgttc gccgaagagg
      661 tcggtctccg tctcttcctt gaaggtggtg gagagcacgc ccgcccgtcc gccgccgaga
      721 gcggaggcgt aggcgaggcc gaggtcgcgc gcctgtcccg aggcgtcctg ctggacggcg
      781 aggaggcacg gaacgccccg tccctcggtg tacatgcgcc ggacgaggtg accggggccc
      841 ttgggggcga ccatgaagac gtcgttgtcc ttggacggct cgacctggtt gaagtggacg
      901 gagaagccgt gcgcgaaggc cagcgccgcg cccttcttca gattaggggc gacgtgcttc
      961 cggtaggtgt cgccctgcac gtggtcgggc atcaggaaca tgaccacgtc ggcgcgcttc
     1021 gtcgcctcgg cgacggtgta cacttcgaag ccgtccttct cggcggtcgc cttcgacttg
     1081 ctgccctcgt ggaggcctac tacgaccgag actccgctgt cgcggaggtt ctgcgagtgc
     1141 gcgtgcccct ggctgccgta gcccagaacc gcgacggtct ttccttcgag aagcttcatg
     1201 tttgcgtcct tgtcgtagag tgcctttgcc ataatcagtc caactccttt tcgaatgtgt
     1261 gctttagaat gtggtggtcg tcgtccgcgc gtcgagggcc agggggccgc tcttcaccgc
     1321 ttcgacgagc ccgagatgcc gaaccgtttc gaggcacgcg tcaagattct cgcccgtgcc
     1381 ggtggtttcg aggatcaccg tgtcgccgtg ctcgcttgcc acggtgtggg ggaaggcgtt
     1441 catcgcgtcg aagagctccg cgcgccgctc cgccggaagg gagaacttca tcagcatcat
     1501 gcagcgctcc acgcagaagc ccgagctgag atcggagact tcgacggtct ccaccatctt
     1561 gcgtatctgc cgcaccgcct gggacgcctt gcggtcgtcg cccgcgatga cgagggtgaa
     1621 gcgggaacgc cccggctgct gcgtgactcc ggcggtgacc cccttcacct gaaatcccct
     1681 gcgctccatg agccccgata tgcgtgaaaa aactcccggt ctgttttccg cgacgacgcc
     1741 gatggtccgt tccatgccgc tctcctcctc ttatcttgtg tatgcccctc tcgggagctc
     1801 tctcttcgcc tgcgaaggcg gtatctgcgc ataaaaaaag ccggggttcc cgtagagtgc
     1861 gggaaccccg gccggaaggc tcttcgtcgg tctacacgac gagaagcgct ccgggggaat
     1921 ccccgcagcg aataataata atggcgccgg ctacgacaaa tgtaagggcg gagtgcgtcg
     1981 ttgtggacat attcttcaca ggggtttcct ccttcgcttc gatatcgtct ttcccgatcg
     2041 tgtgatacca tggagttctc cgagaggggc gctcgttccg cgccgttcgg attgcgggca
     2101 atcttatagg cctccttcga aaatgtcaag gggggttgaa ataaaagata attctaaaat
     2161 tttcagaggg ggttgttcta tggacgctga aaagcttcgt gaatgccggg aaaagatcaa
     2221 cgagatcgac ggaagtatcg ccgcgcttct tt
//
LOCUS       DAOTIL010000436         2252 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminobacteriaceae bacterium isolate AMR_MDS_3026
            NODE_57072_length_2252_cov_2.459718, whole genome shotgun sequence.
ACCESSION   DAOTIL010000436 DAOTIL010000000
VERSION     DAOTIL010000436.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868050
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminobacteriaceae bacterium (wastewater metagenome)
  ORGANISM  Aminobacteriaceae bacterium
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae.
REFERENCE   1  (bases 1 to 2252)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2252)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:19:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,224
            CDSs (total)                      :: 3,198
            Genes (coding)                    :: 3,184
            CDSs (with protein)               :: 3,184
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2252
                     /organism="Aminobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_57072_length_2252_cov_2.459718"
                     /isolate="AMR_MDS_3026"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:3034609"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(31..912)
                     /gene="amrB"
                     /locus_tag="P5201_09205"
     CDS             complement(31..912)
                     /gene="amrB"
                     /locus_tag="P5201_09205"
                     /inference="COORDINATES: protein motif:HMM:TIGR04336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AmmeMemoRadiSam system protein B"
                     /protein_id="HRV98756.1"
                     /translation="MTACVRKLAVLCICLFFAASPLFSEEPPHLQKLRERPFPAAYPG
                     GLFADAIEAAKSTVPASGCVSGLVLPHHLLAADLIAKGFAHVREGGYRRIVILSPDHF
                     SRGKTSFSVPGRDFLTSLGTVPLDKEWTAALLRSELVSESSLFSHEHGVQALLPFIAR
                     HFPGAFVLPVAIGKRSTPEEWRLLAELLTPLLDGVTLIVQSTDFSHYLDREETAKRDE
                     ESLAVLLSADPERVVSLRQPSHIDSRGALFVQMTLQKSLDANPVLLAHASSFEYNPAE
                     AEKTGGTSYITAIYTSE"
     gene            complement(893..>2252)
                     /locus_tag="P5201_09210"
     CDS             complement(893..>2252)
                     /locus_tag="P5201_09210"
                     /inference="COORDINATES: protein motif:HMM:NF022812.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF3160 domain-containing protein"
                     /protein_id="HRV98757.1"
                     /translation="LAQTTSLSGLVVFKVLLQTGRADFLVFLMCGKLAFIWFSKSVNQ
                     AANSIVMNRGLVGRINVTKTLFPLAVVQEGLYRQAAVFLLLFCVLFAWGYTPSLAWFY
                     VVPVIVVNYLMIVACAYIGACLVCLVRDFSMIISLGMTFLLFTSGIFWDVRHLPDPAL
                     TELVLAVNPLAFILDAYRQVLMFHTPPAWMHLLGTLASEFGKGYPLYMQSPLFAAKQL
                     ETQLGSYTELRHDTILYEKPNYAEMGDGGDELPPKPLPKGFVEPNLPFWNELLRVVDY
                     IQNGFEKNVLFPMDMEEYGALTQFRQTVQRCGKIAAKQLQGKALTEEEYEFIRLFSLD
                     YMAAPADGYGAVPPDELFQSGLIVDIQTMNLDPIAIGDPAILYQATAEPSIMLVLVGN
                     ENTPRITIGMAFDHREFTAPHGRRITDSVWKKRVYDKYYDGDPKGLPLPAKNFWYDGL
                     RP"
BASE COUNT          456 a          643 c          757 g          396 t
ORIGIN      
        1 cgggggatga gcgtctcaac gaaagcgcag tcattcggac gtgtagatcg ccgtgatgta
       61 gctggttccg ccggtctttt cggcctcggc tgggttgtac tcgaatgagg aagcgtgcgc
      121 gaggagtacg ggattcgcgt ccagcgactt ctggagcgtc atctgcacga agagcgctcc
      181 ccgggagtcg atatgcgagg gctgccgcag ggagactacg cgctccgggt cggccgaaag
      241 gaggacggcg aggctctcct cgtcgcgctt cgccgtctcc tcgcggtcga ggtagtggga
      301 gaagtcggtg ctctggacga tcagggtaac gccgtccaga agcggcgtga gaagttcggc
      361 aagcagcctc cactcttccg gcgtcgaccg tttgccgatg gcgaccggca gcacgaaagc
      421 gccggggaag tggcgcgcga tgaacggaag gagcgcttgg acgccgtgtt cgtgtgagaa
      481 gaggctggat tcggagacaa gttcgctccg tagcagcgcg gccgtccact ctttatcgag
      541 cgggacggtt cccaaagacg tgagaaagtc acgtcccggg acggagaagg acgtctttcc
      601 ccggctgaag tggtcggggg agaggatgac gatgcgccgg taccctccct cgcgcacgtg
      661 cgcaaacccc ttcgcgatca ggtcggccgc gaggagatgg tgcgggagga cgagtccgga
      721 gacgcagccg gaagcgggca ctgttgattt cgcggcctcg atggcgtctg cgaagaggcc
      781 gccggggtac gccgcgggga acgggcgttc gcgcagtttt tgaaggtgcg gcggctcctc
      841 cgaaaagaga ggagacgccg caaaaaacag gcagatacag aggacggcga gtttacggac
      901 gcaggccgtc ataccagaag ttcttcgccg gtagcggcag ccccttgggg tcgccgtcgt
      961 agtacttgtc gtagacgcgc ttcttccaga cgctgtcggt gatgcgccgt ccgtgcgggg
     1021 cggtgaactc gcggtggtcg aacgccatgc cgatcgtgat tcgaggcgta ttttcgttgc
     1081 cgacgagcac cagcatgatc gaaggctcgg cggtcgcctg gtagaggatg gccgggtcgc
     1141 cgatagcgat cgggtcgagg ttcatggtct ggatatcgac gatgagtccg gactggaaga
     1201 gctcgtccgg aggcaccgcg ccgtagccgt ccgccggggc ggccatgtag tcgagcgaaa
     1261 acagacggat gaactcatac tcttcttcgg tgagcgcctt gccctgaagc tgcttcgctg
     1321 cgatcttgcc gcatcgctgc accgtctgcc ggaactgggt gagcgcgccg tactcctcca
     1381 tatccatcgg gaagaggacg tttttctcaa aaccgttttg gatatagtcg acgacgcgga
     1441 gcagctcgtt ccagaaggga aggttcggct cgacgaaacc cttgggaagc ggctttggag
     1501 gaagttcgtc gcctccgtcg cccatttcgg cgtagttcgg cttctcgtag agtatggtgt
     1561 cgtgccgcag ctcggtgtac gagccaagct gcgtctcaag ctgcttggcg gcgaagagcg
     1621 ggctctgcat gtacagcggg tacccctttc cgaactcgga ggcgagcgtt cccagcaggt
     1681 gcatccaggc cggcggagtg tggaacatca acacctgccg ataggcgtcc aggatgaacg
     1741 ccagcgggtt gaccgccagc accagttcag tgagggccgg gtctggcaaa tgccgcacgt
     1801 cccagaaaat ccccgaggta aagagcagga acgtcatgcc cagggaaata atcatgctga
     1861 aatccctcac caggcagacc aggcaggcac cgatataggc gcaggcgacg atcatcaggt
     1921 aattgaccac gataaccggc accacataga accaggccag cgagggcgta taaccccagg
     1981 cgaacagcac gcagaacaac agcaggaaca ccgccgcctg gcgatacagt ccctcctgca
     2041 ccaccgccag cgggaacagg gtcttggtga cattgatacg gcccaccagt ccgcgattca
     2101 tgacgatgct gttggcggcc tggttcaccg atttcgaaaa ccagatgaag gccagcttgc
     2161 cgcacatgag gaataccagg aaatcggctc gccccgtttg cagcagcacc ttgaagacga
     2221 cgagccccga gaggctcgtc gtctgcgcca gc
//
LOCUS       DAOTIL010000437         2249 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminobacteriaceae bacterium isolate AMR_MDS_3026
            NODE_57155_length_2249_cov_3.518687, whole genome shotgun sequence.
ACCESSION   DAOTIL010000437 DAOTIL010000000
VERSION     DAOTIL010000437.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868050
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminobacteriaceae bacterium (wastewater metagenome)
  ORGANISM  Aminobacteriaceae bacterium
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae.
REFERENCE   1  (bases 1 to 2249)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2249)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:19:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,224
            CDSs (total)                      :: 3,198
            Genes (coding)                    :: 3,184
            CDSs (with protein)               :: 3,184
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2249
                     /organism="Aminobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_57155_length_2249_cov_3.518687"
                     /isolate="AMR_MDS_3026"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:3034609"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..577
                     /locus_tag="P5201_09215"
     CDS             <1..577
                     /locus_tag="P5201_09215"
                     /inference="COORDINATES: protein motif:HMM:NF024975.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HRV98758.1"
                     /translation="IVRGKAGAKVEFGAKILVAIENGHAFVVHSSWENFNESTHFVEG
                     CVQYRERNGYWPEVVCADMIFRTRANLAWCRERGIRLSGPGLGRPPLDEGKLEERKLL
                     ERKDAAKRNEVEGKFGEGKRKYGLDRIRGKLPETSDSIIILQFIIMNLWRMLRDILSF
                     FLESVEKVVLVMSWLSLIHNLATFALPKQGS"
     gene            746..2158
                     /locus_tag="P5201_09220"
     CDS             746..2158
                     /locus_tag="P5201_09220"
                     /inference="COORDINATES: protein motif:HMM:NF024935.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HRV98759.1"
                     /translation="MTPILHHADSDDDTLCSAAAFFHTFGISGLFNRSARTGKGIPPV
                     RILQFLFALVFLRKTFFEMVRENCLPWGKDTFYRFLSESRINWRRFLLLVAKRLLDTF
                     FLPLTTERRDRVLILDDSIYSRNRSKKVELLSKCYDHCAHRYFRGFRMLSLGWSDGRS
                     FLPLAFSLLGSAKEEKRFFGAADTDGRTSGARRRKEAVMKTSEAALILLDAALQNGIK
                     ADFLLFDSWFATNQFLHSVHRKGIRSIAMAKDFNSLQFLAVQKDGTKKRMKLADLYQQ
                     ASPGLDRKDIPGSVLVEVDSGKDSQEAPLPVKVVFVRNRKSGAKREWLAVLCTDTSVP
                     DEEVVRIYGKRWSIEVFFKACKSVLRLSKEFQTRSYDSMVAHTSIVFLRYMMLSFELR
                     RSEDDRAYGELFCRCCEELEDISFVASLALLLEMLRSAVRAGIDMTETILERMFTEFV
                     RKTPGFLRNKIGILAAFSMS"
BASE COUNT          530 a          574 c          625 g          520 t
ORIGIN      
        1 gatagtgagg ggcaaagcgg gagcgaaagt cgaattcgga gcgaagatcc tcgttgccat
       61 agagaacgga cacgcctttg tcgttcactc gtcgtgggaa aacttcaacg aaagcacgca
      121 cttcgtcgag ggatgcgtcc agtaccggga acggaacgga tactggccgg aagtggtatg
      181 cgcggacatg atcttccgaa cgcgggccaa cctggcgtgg tgccgggagc gaggaatccg
      241 gctcagcggc ccggggctcg gaagaccgcc gcttgacgag gggaagctcg aggagcggaa
      301 gttgctggaa aggaaagacg cggcgaagag gaacgaagtc gagggcaagt tcggagaagg
      361 gaagaggaag tacggtctgg accggatacg ggggaaactt cccgaaacaa gcgacagtat
      421 tattatcctt cagttcataa taatgaatct ttggaggatg ctccgggata ttttgtcttt
      481 ttttctggaa agcgtcgaaa aagtcgtttt ggtcatgtcg tggttgagtc ttatccacaa
      541 cttggccact tttgctttac cgaagcaagg ctcgtaactt gttcaggatg ccctaaatcg
      601 attttccctt tctatttttc tcaactttcg caggaccttg acagccgaaa aaccgttgaa
      661 atgactggat aaaaatgaca ggaaggcccc tgttttgtaa aattgtagtc gcgagataac
      721 aaaactacaa aaaggaggcc ttcccgtgac tcccatttta catcatgccg actctgacga
      781 cgatactctt tgttccgccg cagcgttttt tcacaccttc ggcatctcag gactcttcaa
      841 ccgctcagcc aggacaggaa agggaattcc ccccgtcagg atcctgcagt ttctcttcgc
      901 cctcgtcttc ctcaggaaga ccttcttcga gatggtgcgc gagaactgcc tgccctgggg
      961 caaggacacg ttctaccgct tcctctcgga atctcggatc aactggaggc gcttcctcct
     1021 gctcgtcgcg aagaggcttc tcgacacctt ctttctccct ctcacgacgg agagaagaga
     1081 ccgggttctg attctcgacg attccatcta ctcgaggaac agaagcaaaa aggtggaact
     1141 tctgtcgaaa tgctacgacc actgtgctca ccgatacttc agaggtttcc gcatgctctc
     1201 cctcggctgg tccgacggaa ggtcctttct gcccctggca ttctcccttc tcggttcggc
     1261 caaggaggag aaacgattct tcggagccgc ggacaccgac ggaagaacat cgggggcacg
     1321 gagacggaaa gaagcggtga tgaagacgtc ggaagcggct cttatccttc ttgacgctgc
     1381 gctccagaac ggcataaagg ccgactttct tctcttcgat tcctggtttg ccaccaacca
     1441 gtttcttcac tccgtgcaca ggaaaggcat ccgttccatc gccatggcca aggacttcaa
     1501 ttccctgcag ttcctggcag tgcagaagga cggaacgaag aagcggatga agctcgcaga
     1561 cctgtatcag caggcctccc cgggattgga tagaaaggat atccccggtt ccgtgctcgt
     1621 ggaggtagac agcgggaagg actcccagga agctcccctg ccggtcaagg tcgtgttcgt
     1681 gagaaacagg aagagcggcg cgaagcgtga gtggctggcc gttctgtgca ccgatacttc
     1741 cgttcccgac gaagaagttg tccggatcta cgggaagcga tggtccatag aggtcttctt
     1801 caaggcctgc aagagtgttc tgcggctgtc gaaggagttt cagaccagga gctacgactc
     1861 catggtggcc cacacgagca tcgtcttcct gaggtacatg atgctctcct ttgaactgag
     1921 gagatccgag gatgaccgtg cgtacggaga actcttctgc agatgctgcg aagagctgga
     1981 agacatatca ttcgtcgcgt cgctcgctct gcttctggag atgctgagat ccgccgtcag
     2041 ggccggaata gacatgacgg aaaccatcct ggaacggatg tttacggaat tcgtacggaa
     2101 aacgcccggc ttcctgagga ataaaatagg tattttagct gctttttcca tgtcatgaca
     2161 acgatttacg gttttcaagg tcctgagaat gttgagaata ctttttgtga cgttctgaag
     2221 tttgacgatt taccgcacat tttctattt
//
LOCUS       DAOTIL010000438         2244 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminobacteriaceae bacterium isolate AMR_MDS_3026
            NODE_57305_length_2244_cov_4.019187, whole genome shotgun sequence.
ACCESSION   DAOTIL010000438 DAOTIL010000000
VERSION     DAOTIL010000438.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868050
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminobacteriaceae bacterium (wastewater metagenome)
  ORGANISM  Aminobacteriaceae bacterium
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae.
REFERENCE   1  (bases 1 to 2244)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2244)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:19:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,224
            CDSs (total)                      :: 3,198
            Genes (coding)                    :: 3,184
            CDSs (with protein)               :: 3,184
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2244
                     /organism="Aminobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_57305_length_2244_cov_4.019187"
                     /isolate="AMR_MDS_3026"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:3034609"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..731)
                     /locus_tag="P5201_09225"
     CDS             complement(<1..731)
                     /locus_tag="P5201_09225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015555937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide/oligopeptide/nickel ABC transporter
                     ATP-binding protein"
                     /protein_id="HRV98760.1"
                     /translation="MTKLLEVTNLEQRFDLNTDFLSKLVFEKGRIVKRERIVKAVNGV
                     SFSVGRREVFSIVGESGCGKSTVARTVVKLIEPKGGRICYDGRDITELSRTEMLPFRK
                     RMQMIFQDPYASLNPRQRVADILVEPMLFHKIARSPEEAREKALEILNRVGIRPEQAS
                     RYPHQFSGGQRQRIGIARALAVEPEFIVADEPVSALDVSIQAQILNLLMDLKEEFSFS
                     YLFIAHDFSVVKHISDNLAVMYLGR"
     gene            complement(728..1726)
                     /locus_tag="P5201_09230"
     CDS             complement(728..1726)
                     /locus_tag="P5201_09230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009165884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HRV98761.1"
                     /translation="MNDTLLEVKNLRTYFHTFKGTVKAVDDVSFSVGRGEILAIVGES
                     GGGKSVTGFSVIRLIEEPGVIESGEILFEGRDVMKLSEREVDRLRGKEISMIFQDPMT
                     SLNPVYTIGRQIEESLLLHTKLNREERRAACIDLLRSVGISNPESRLKAYPHQFSGGM
                     RQRVVIAIALAADPKLIIADEPTTALDVTIQAQILRLMADLVRAKNRSMILVTHDLAV
                     VSEMADRINVMYCGKIVETGSARKVIDESAHPYTKGLIDSIPDLERDRKRLNTIKGIV
                     PNMFELPEGCNFAPRCARATELCFGVEPEQREIGAGHFVACHFPLGGPAHEEEARS"
     gene            complement(1742..>2244)
                     /locus_tag="P5201_09235"
     CDS             complement(1742..>2244)
                     /locus_tag="P5201_09235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005660871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HRV98762.1"
                     /translation="MRIADIQLSFPTMLIALFLMSVLGRGVTNILISLTLVGWVRFAR
                     TVRGETLSVKKREYVEAARVIGLPNVRILFKHVLPNVFASIIVLATIQVGNFILTEAT
                     LSFLGLGVPITRPSLGMLCNNGFTVLYSGLWWVSILPGLYIMIIVFGINLLGDFLRDE
                     LNPKLK"
BASE COUNT          400 a          700 c          712 g          432 t
ORIGIN      
        1 atcctcccga ggtacatcac cgcgaggttg tcgctgatgt gcttcaccac ggagaagtcg
       61 tgcgcgatga agaggtacga gaaggaaaac tcttccttca ggtccatcag cagattgaga
      121 atctgcgcct ggatcgagac gtcgagcgcg gagaccggct cgtcggcgac gatgaactcc
      181 ggttccacgg cgagcgcgcg cgcgatgccg atccgctgcc gctgccctcc gctgaactgg
      241 tgcgggtagc gcgacgcctg ctcgggccgg atgccgaccc tgttcagaat ttcgagcgct
      301 ttctcccgcg cctcctccgg cgagcgcgca atcttgtgga agagcatcgg ctcgacgagg
      361 atgtccgcca cccgctgcct tggattcagc gaggcgtagg ggtcctggaa gatcatctgc
      421 atccgtttcc ggaaggggag catctccgtg cgggagagtt ccgtgatatc cctgccgtcg
      481 tagcaaatcc gccccccctt cggctcgatg agcttcacca cggtgcgggc gacagtcgac
      541 ttgccgcacc cggattcgcc cacgatgctg aagacctcgc gcctgccgac ggagaagctg
      601 acgccgttga cggccttcac gatccgctcg cgcttgacga tacgtccctt ctcgaagaca
      661 agcttgctca agaagtcggt attcaggtcg aagcgctgtt ccaggttcgt cacttcaagc
      721 agtttcgtca tgagcgcgcc tcctcttcat gcgccggccc gcccagcggg aagtggcagg
      781 cgacgaaatg ccccgccccg atttctctct gctccggctc gacgccgaag cacagctccg
      841 tagcgcgcgc gcaacggggg gcgaaattgc acccctcggg cagctcgaac atgttgggga
      901 cgatgccctt gatggtgttc agcctcttcc tgtcgcgctc caggtcgggg atggagtcga
      961 tcagcccctt cgtgtacggg tgcgcgctct cgtcgatgac cttccgcgcg gaaccggtct
     1021 ccacgatctt gccgcagtac atcacgttga tccggtcggc catctccgag acgacggcga
     1081 ggtcgtgggt gaccaggatc atcgagcggt tcttcgccct gacaaggtcg gccatcaggc
     1141 ggaggatctg cgcctgtatc gtcacgtcga gcgccgtggt gggttcgtcg gcgatgatca
     1201 gcttggggtc cgcggccagc gcgatggcga tgaccacccg ctgccgcatg ccgccgctga
     1261 actggtgcgg gtaggccttg agcctgctct cggggttgga gatgccgacc gaccgcagca
     1321 ggtcgatgca ggccgcgcgc cgctcctccc tgttcagctt cgtgtggagc agcagacttt
     1381 cctctatctg ccgtcctatg gtgtacacgg ggttcagcga cgtcatcggg tcctggaaga
     1441 tcatggagat ctccttcccc cggagacggt ccacctcgcg ctcggagagc ttcatcacgt
     1501 cgcggccctc gaagaggatc tcccccgact cgatgacgcc gggctcttcg atcaggcgga
     1561 tgacggaaaa gccggtgacg gactttccgc ctcccgattc gccgacgatg gcgaggattt
     1621 cgccccgccc gacggagaag gagacgtcgt cgaccgcctt gacggtccct ttgaacgtgt
     1681 ggaagtacgt gcgcaaattc tttacttcaa gcaacgtgtc gttcattcgg tccgtccctc
     1741 ctcacttgag cttcgggttc agctcgtccc tcaggaagtc gcccagaagg ttgatgccga
     1801 agacgatgat catgatgtag agccccggca ggatggagac ccaccagagt ccgctgtaga
     1861 gcaccgtgaa gccgttgttg cagagcattc ccagcgaggg gcgggtgatg gggacgccaa
     1921 gtccgaggaa gctcagggtg gcctccgtga ggatgaagtt gcccacctgg atcgtggcga
     1981 ggacgatgat cgaggcgaag acgttgggca gaacgtgctt gaagagtatc ctgacgttcg
     2041 gaagaccgat gacacgcgcg gcctcgacgt actcgcgctt cttgaccgag agcgtctcgc
     2101 cgcgcaccgt ccttgcgaag cggacccacc ccaccagcgt cagcgagatg aggatgttgg
     2161 tcacgcccct tccgaggacg gacatcagga agagcgcgat gagcatggtc gggaaggaga
     2221 gctgaatgtc cgcgatccgc atga
//
LOCUS       DAOTIL010000439         2243 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminobacteriaceae bacterium isolate AMR_MDS_3026
            NODE_57358_length_2243_cov_2.862431, whole genome shotgun sequence.
ACCESSION   DAOTIL010000439 DAOTIL010000000
VERSION     DAOTIL010000439.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868050
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminobacteriaceae bacterium (wastewater metagenome)
  ORGANISM  Aminobacteriaceae bacterium
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae.
REFERENCE   1  (bases 1 to 2243)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2243)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:19:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,224
            CDSs (total)                      :: 3,198
            Genes (coding)                    :: 3,184
            CDSs (with protein)               :: 3,184
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2243
                     /organism="Aminobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_57358_length_2243_cov_2.862431"
                     /isolate="AMR_MDS_3026"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:3034609"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            324..>2243
                     /locus_tag="P5201_09240"
     CDS             324..>2243
                     /locus_tag="P5201_09240"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRV98763.1"
                     /translation="MIGATFGGTPSMRLGTLMKAADLEAISGRRVVVLSALPEVPPVL
                     GCSPRLLAPSFEQSHPVDIAAECYAAVSGADAAARLFAEGCLSTEGASVNRTSNDRHW
                     SGNLRLGLQYLFRASGILSDRTGDSLARTLVGCVDDLNSLTGERSEHKSSSKALQWMD
                     ALPPEIRRQLEGIYLKGHADTVRSHFRTLMPLLEEIRATAPDMWTGPFLADPHGEPIY
                     VYTPDWGEGPLALLLRVCNMSARAFFILPELHRWAPLEISRVGYAFSTRRGSIDAHYS
                     SAFPLSASGFDADWELYGATSSMSVLSFFRQRLAATGAGDERLKNLAVETPSALDERH
                     AILRNSAGWSLVECDGFDMKRLFFLKRQGEQDSYKERFDSNIASERGMALKRSAAPAA
                     GRRSRSSVEAENVVCVEGVPELQSLPDKGDALYSLEGRSFFVDREVRISPRRHVALLP
                     AGEDEPGYPLILCEKLWTAEQSAGFTSPEPRMISWLSFETTRDTQIHPEKAGQPGKRR
                     VTVSAPFLEHDVTLVIPASRSLFPCAGNKSGIIASGAWLEPESVFLVQELLVVRAPEL
                     KDEPRRPFLAHPLQEKCLDAGRELAMHADALLEELREGRGFGESEKAEADFFRSKKKY
                     VDTARALVKEFFGALA"
BASE COUNT          412 a          604 c          732 g          495 t
ORIGIN      
        1 cgccctcttc tgaggctgag ttcgactttt gtgaacacgt gccttcacat ggtccggatc
       61 tggaattgcg ggagcggatt gcggccggaa aacgggatgt tttctccccc tccatccgtt
      121 ccttttatcg cgtttctgct tgcgcctacg ggatttcagt ttacggaaga agagatggat
      181 tccgtacgtg acgaatcggg gtacggggtt gaggatgtcc gcaaatggca cgaaaaggtg
      241 atgagtcgtc tggaaaaaag aagaggggct acgccgcgtg tctgcgagcc gggaaacgga
      301 gcggaaagaa agggggaggc gtcatgatag gtgcaacttt cggaggaact ccctccatgc
      361 gtctgggtac gcttatgaaa gcagccgatc tggaggcgat ctccggacgc agggttgttg
      421 tgctcagcgc tctgccggag gttccgcctg ttttgggttg ctctccccgt cttcttgcgc
      481 cgtctttcga gcagtcgcac cccgtcgata tcgccgcaga gtgctacgcg gcggtctccg
      541 gcgcagatgc ggcggccaga ctgttcgccg agggctgtct ttcgacggaa ggggcctccg
      601 taaaccgcac ttccaacgac cgtcactggt cggggaatct ccggctgggt ttgcagtatc
      661 ttttccgcgc atccggaatt ctttcggatc gcaccgggga ttctcttgcc cggacacttg
      721 tgggctgcgt cgacgatctg aattctctta cgggcgagag atccgaacac aaaagctcct
      781 cgaaggcgct tcagtggatg gatgcgcttc cgccggagat tcgacggcaa ctggagggga
      841 tctacctgaa agggcatgcc gacactgtga gatcccattt taggacgctg atgccgctcc
      901 ttgaggagat acgcgccaca gcccctgata tgtggaccgg gccctttctg gcagaccccc
      961 acggagagcc gatctacgtt tatacgcccg actgggggga ggggccgctc gcgctgcttc
     1021 tccgcgtttg caatatgagc gcgcgggcat tcttcatcct tccggagctt caccggtggg
     1081 cgccccttga gatttcgcgc gtggggtatg ccttcagtac ccggaggggg agcatcgacg
     1141 cgcactattc ttccgctttt cccctttccg cttccggttt tgatgcggat tgggagctgt
     1201 atggcgcgac ttcgtccatg agcgtactct cattcttcag gcaacgcctt gcggcgacgg
     1261 gagcgggaga tgagcgtctg aaaaatctcg ctgtcgaaac accctccgcc cttgatgagc
     1321 ggcacgccat cctccgcaac agtgcggggt ggtcgttggt cgagtgcgac gggttcgaca
     1381 tgaagcgtct tttcttcctg aagaggcaag gggagcagga ttcgtacaag gagcgcttcg
     1441 acagcaacat cgcaagcgag cgggggatgg cgttgaagag gagtgccgcc cccgctgcgg
     1501 gacgaaggtc gcgttcttcc gtagaagcgg aaaacgtcgt ctgcgtggaa ggcgttccgg
     1561 agcttcaatc gcttcccgat aagggggacg cgctctattc tctcgaaggg cggagtttct
     1621 tcgtcgaccg ggaggttcgc atttcaccaa gacgccacgt cgctcttctt ccggcagggg
     1681 aggatgagcc gggatacccc ttgattcttt gcgaaaagct gtggacggct gagcagtcag
     1741 ccggatttac ctctccggaa ccgcgtatga tctcctggct ttcattcgag acgacgcggg
     1801 acacgcaaat tcatccggag aaggcgggac agccggggaa aagaagggtg acggtttcgg
     1861 ctccttttct ggagcacgat gtgaccctgg tgataccggc gtcgcgttcg ctttttccgt
     1921 gcgcgggaaa caaatccggg ataattgcgt cgggggcatg gctcgagccc gaaagcgtct
     1981 ttctggtgca ggagctgctg gttgtccgcg ctccggagct gaaagatgaa ccgcgccgtc
     2041 ccttcctcgc gcatccgttg caggagaagt gccttgacgc gggcagggag cttgccatgc
     2101 atgcggatgc gctgctggaa gagctgcgcg agggacgggg gtttggggag tccgaaaagg
     2161 cggaggcgga cttttttagg tcgaagaaga agtacgtgga caccgcacgc gctttggtga
     2221 aggaattctt tggtgcgctg gcg
//
LOCUS       DAOTIL010000440         2242 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminobacteriaceae bacterium isolate AMR_MDS_3026
            NODE_57396_length_2242_cov_3.172840, whole genome shotgun sequence.
ACCESSION   DAOTIL010000440 DAOTIL010000000
VERSION     DAOTIL010000440.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868050
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminobacteriaceae bacterium (wastewater metagenome)
  ORGANISM  Aminobacteriaceae bacterium
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae.
REFERENCE   1  (bases 1 to 2242)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2242)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:19:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,224
            CDSs (total)                      :: 3,198
            Genes (coding)                    :: 3,184
            CDSs (with protein)               :: 3,184
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2242
                     /organism="Aminobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_57396_length_2242_cov_3.172840"
                     /isolate="AMR_MDS_3026"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:3034609"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            147..938
                     /locus_tag="P5201_09245"
     CDS             147..938
                     /locus_tag="P5201_09245"
                     /inference="COORDINATES: protein motif:HMM:NF014691.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fic family protein"
                     /protein_id="HRV98764.1"
                     /translation="MDGYSRAVRLWQSYGIASAADLDKYLHSFRILFAYHSGKIENEE
                     ITYHDTREIFENGRVTGYTGSPRALFEQQNQKLCYEFLKEKIVSLDPLDIKLVKETHR
                     LLTAGTYDERRYIDNGERPGEFKKHDYVTGVHEVGSSAEDVEKEVAELIAEIDAYEGD
                     DVLKAAAYFHARFEYIHPFADGNGRVGRTLLNYYLMIRGHPPLIVHDEEKGTYYAALQ
                     QYDEAENLAPLYEFFRYETEKTWAKALTLLDGTKPDRKGLSDLLN"
     gene            963..1364
                     /locus_tag="P5201_09250"
     CDS             963..1364
                     /locus_tag="P5201_09250"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRV98765.1"
                     /translation="MSTGDAERNKQPWSQNIPRTVLVFAPRILGIGFILFISLFALDV
                     FSEDADAWSMCLGFMIHMIPSAILAVVLVISWRFELFGAAAYFILALSYYVLTKGKED
                     LLAYISISGPMLLISALFFFNWLQIKRRKGE"
     gene            complement(1507..>2242)
                     /locus_tag="P5201_09255"
     CDS             complement(1507..>2242)
                     /locus_tag="P5201_09255"
                     /inference="COORDINATES: protein motif:HMM:NF012772.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HRV98766.1"
                     /translation="LFSGIKRLLFFDRRGWCDCWRGSFLRSLRSRSARLVEEHATDDT
                     GADIAQQVFEHFRSLAFVLNERIALGVGFQPDGYPQGIHAGQVFHPQLRDHAQQNMAV
                     DLILYPPTEQILELELTESIFVEDKGIAVLNKLKEMEIQIAIDDFGTAYSSFNRLREM
                     AIDCLKIDRSFLCGLGGEDRVRDEGIVRAIIAMGRSMDLRVIAEGVETSKQADFLKEN
                     HCQEAQGFLFSKPVPAQQIKKIFESH"
BASE COUNT          580 a          555 c          568 g          539 t
ORIGIN      
        1 aagtgcggct ttcgaattac gggcgtcgaa ttcgactact tcagaaggtg ctgtacggaa
       61 aagatttttg aaaacggaat agagtgccgg gatatgatca ggatggagat gcacctggac
      121 gaagagacgc aaaaagaggg atgaatatgg acggatacag ccgtgcagta cgcctgtggc
      181 agtcgtacgg aatcgcgtcg gccgccgatt tggataaata cctccacagc ttccgcattc
      241 tgttcgcgta ccattccgga aagatagaga acgaagagat cacctatcac gacacgcggg
      301 agatcttcga aaacggcaga gtgacgggct ataccggaag cccccgcgct ctcttcgagc
      361 aacagaatca aaagctgtgt tacgaatttc tgaaagagaa gatcgtgagc ctcgacccgc
      421 tcgacataaa attagtgaaa gaaacgcatc gcttgctgac cgccggaaca tacgacgaac
      481 gcaggtatat tgataacggt gagcggccgg gtgaattcaa aaagcacgat tacgtaaccg
      541 gagttcatga ggtcgggtcg tccgccgaag atgtggaaaa agaagtcgcg gagctgatcg
      601 ccgagataga cgcctacgag ggcgatgacg tcctcaaggc ggccgcctat tttcacgcga
      661 ggttcgaata tatccacccc ttcgccgacg gcaacggacg cgtcggcaga acccttctga
      721 actactacct catgatccgc ggccatcccc cccttatcgt ccacgacgag gagaagggaa
      781 cgtattacgc ggccctgcag cagtacgacg aagcggagaa tttggcccct ctgtacgagt
      841 ttttcaggta tgagactgaa aaaacgtggg cgaaagcctt gaccttgctt gacggcacaa
      901 agcccgatcg caaaggtctg tcggatttat taaattagga ttgcggaaag gagaatacag
      961 ccatgtcgac gggggatgcg gagcgtaaca aacagccttg gtctcaaaat ataccgcgga
     1021 ccgtgcttgt gtttgctccg cggatactcg ggataggatt tattttattc atcagcctct
     1081 ttgctcttga tgtcttttcc gaagatgccg atgcatggtc gatgtgtttg ggatttatga
     1141 ttcatatgat cccctccgcg attctcgcag ttgttcttgt tatctcatgg cgtttcgaac
     1201 tgttcggcgc agcggcatat ttcattcttg ctttgtccta ttacgtcttg accaagggga
     1261 aggaagacct gctcgcatac atttccattt cggggccaat gctcctcata agcgcgctct
     1321 tttttttcaa ttggctacag atcaagaggc gaaaaggaga gtgatagaaa accccttgcc
     1381 gtagaaggtc aagcagtgag ctggaaaatt cgttgtcctt tttgtgaaaa atccttttcg
     1441 aaaaaactta tttaaagatg tgccgtggta aagagagctt catggcgtct tcatgaagcc
     1501 ctctcttcaa tgcgattcaa aaattttttt aatttgctgt gccggtaccg gcttgctgaa
     1561 gaggaagcct tgagcttcct gacagtgatt ttctttcaga aaatcagcct gtttacttgt
     1621 ttcaactcct tcggcgatca ctcgcaagtc catacttcgg cccatggcaa taatcgcacg
     1681 aacgatgcct tcatctcgaa cacggtcttc accgcctaat ccgcataaga aggagcgatc
     1741 gattttcaga cagtcgatag ccatctctct cagacggtta aaacttgaat aggctgtgcc
     1801 gaagtcatca atggcgattt gaatttccat ctctttgagt ttattcagta cagcaattcc
     1861 cttatcctca acaaaaatgc tttcggtaag ctcaagctcg agaatttgct cggtaggcgg
     1921 gtacagaatc agatccaccg ccatattctg ttgagcgtga tctctgagct gtgggtggaa
     1981 tacctgaccc gcgtggatgc cctgcgggta tccatcgggt tggaagccta cgcccaacgc
     2041 gatcctctcg ttcaatacaa aggccaggct tcggaaatgt tcaaaaacct gttgagcgat
     2101 atccgctccg gtatcatcag tcgcatgttc ctctaccagc cgcgcagacc gggagcgcaa
     2161 actgcggaga aaactgcccc tccagcagtc acaccagccc cggcggtcaa agaacagcag
     2221 gcgcttgatc cccgaaaaaa ga
//
LOCUS       DAOTIL010000441         2236 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminobacteriaceae bacterium isolate AMR_MDS_3026
            NODE_57613_length_2236_cov_4.019257, whole genome shotgun sequence.
ACCESSION   DAOTIL010000441 DAOTIL010000000
VERSION     DAOTIL010000441.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868050
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminobacteriaceae bacterium (wastewater metagenome)
  ORGANISM  Aminobacteriaceae bacterium
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae.
REFERENCE   1  (bases 1 to 2236)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2236)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:19:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,224
            CDSs (total)                      :: 3,198
            Genes (coding)                    :: 3,184
            CDSs (with protein)               :: 3,184
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2236
                     /organism="Aminobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_57613_length_2236_cov_4.019257"
                     /isolate="AMR_MDS_3026"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:3034609"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(126..557)
                     /locus_tag="P5201_09260"
     CDS             complement(126..557)
                     /locus_tag="P5201_09260"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRV98767.1"
                     /translation="MDSLLEYLEFFLKFVLPALSAIAVWYWNERCKRLADQYQRKEAA
                     YRELIRALRGFYVGAENADSLKEEFLNQLNLTWLYCPDDVIKKGYAFLDTVHVRQVRT
                     DEEKEAALGEFVYAIRKDLLSRNLLRCTSLTPADFKHLNIM"
     gene            782..>2236
                     /locus_tag="P5201_09265"
     CDS             782..>2236
                     /locus_tag="P5201_09265"
                     /inference="COORDINATES: protein motif:HMM:NF023990.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3754 domain-containing protein"
                     /protein_id="HRV98768.1"
                     /translation="MWRRFVSDDGFFGRALPRRGRVELFNTGGVAMAVTPDLGKFIPV
                     RTEDLVDVLCEEQTLSAEDREKFRRVAELVEATYHYDFHSTLKEARGAYHIFNPDADT
                     LKICPPGESMDARFDSMVRALEEILVAANYREFPIAELNELMTKAAPKGLNVRVNLEN
                     YEKILIYRRGTKQVPKPKEPLSWWKSLRKKDAPVEPEMEEMIQRLLILIKLKSQEDME
                     QFYDTFIKTAAETENERERRMTREAVLGKKEKETAAKKSNGAPKVPTIFLKVFKNVPA
                     STLQTLFPDVTIQMTLIDKGKIALPIIAGFLSVVNRVIPALLVVGTLIAAIVAGRNID
                     WAHFKEQLFPILAAFSIVGTIAFKVFTKYKLTKEKHQAKLMKTLYFHNLDNNAGVFDF
                     LVHEAEEEECKEALLAYYFLLTGKDSEGKAYSQDELDDRIEQWMKEKFGVTLDFEVDD
                     ALRKLEDKKLLVKEGDRYLVPSIDDTLVKLDELWD"
BASE COUNT          590 a          603 c          594 g          449 t
ORIGIN      
        1 gaaaaaacgt atcgaagaga cgctgatgag aatacggcag gcgcttgagc gggtgtgaag
       61 tgaaacatgc gatgcacatt cttcccaggg aacaaccttc aaagaaagct tactattaaa
      121 acggtctaca taatgttaag atgcttaaaa tcagcaggag taagagatgt acagcgtaga
      181 agattacgcg acaggagatc ttttcgtatt gcatatacaa attcgcctaa tgcagcttct
      241 ttctcttcat ccgttctcac ttgacgaaca tgcaccgtgt ccaaaaacgc ataacctttt
      301 ttgatgacgt catccggaca gtaaagccac gttaagttga gttggttaag aaactcttct
      361 ttgagagaat ctgcattctc tgcacccaca taaaaacctc tcaatgctcg gatcaactct
      421 cgatatgctg cttccttccg ttgatactga tcggcaaggc gtttacaccg ttcattccaa
      481 taccaaacag ctatagcgct taaagctgga agcacaaact ttaaaaaaaa ctccagatac
      541 tcaagaagag aatccacaga tcaacgcccc ctaatatgag atagatgatt caacaaaaaa
      601 aggagaaaat tcttaaaccc ctcattccat taagcagaga acatcctaca aactactcat
      661 tcgcaaatga gcggatagcg gctgttcgtc cgtcaatcct tttttctccc catttctacc
      721 acaccccgca caattcggca agcttttcac actattgata aacaggccgg atgaatgtac
      781 tatgtggaga cggtttgttt ccgacgacgg attcttcggg cgggctcttc cccgcagagg
      841 acgcgtcgaa ctttttaata caggaggggt tgcgatggcg gttactccgg atctgggcaa
      901 gttcatcccc gtgcgcaccg aggaccttgt ggacgttctg tgcgaggagc agaccctttc
      961 ggcggaggac agggagaagt tccgccgcgt cgccgaactg gtcgaggcga cgtaccacta
     1021 cgactttcac agtacgctga aagaggcgcg cggcgcctac catatcttca accccgacgc
     1081 cgacaccctg aaaatctgtc cgcccggcga aagcatggac gcccgcttcg acagcatggt
     1141 gagggcgctc gaagaaatcc ttgtagcggc gaactaccgc gagtttccca tcgccgagct
     1201 gaacgaactg atgacgaaag cggcccccaa ggggctgaac gtgcgagtca accttgagaa
     1261 ctacgagaag attctcatct accggcgggg aacgaagcag gttccaaagc cgaaggaacc
     1321 gctctcctgg tggaagtcgc tccggaagaa ggacgcgccc gttgaaccgg agatggagga
     1381 gatgatccag cggctgctca tcctgatcaa gctgaagagt caggaggaca tggagcagtt
     1441 ctacgacacc ttcatcaaga ccgccgcgga gaccgagaac gaacgcgagc gccgcatgac
     1501 tcgcgaggcg gtgctcggca aaaaggagaa ggaaactgct gcgaagaaga gcaacggcgc
     1561 gccgaaggtg cctacgatct tcctgaaggt cttcaagaac gttccggcct cgacgctgca
     1621 gacgctcttc cccgacgtga ccatccagat gaccctgatc gacaagggaa agatcgctct
     1681 gccgatcatc gcgggctttt tgagcgtggt gaaccgcgtc atccccgcgc tgctcgtcgt
     1741 cggcacgctg atcgccgcga tcgtcgccgg acggaacatc gactgggcgc acttcaagga
     1801 gcagctcttc ccgatcctcg cggccttctc catcgtcggg acgatcgcct tcaaggtctt
     1861 cacgaagtac aagctcacga aggagaagca ccaggcgaag ctgatgaaga cgctctactt
     1921 ccacaacctg gacaacaacg ccggagtctt cgactttctc gttcacgagg cggaggagga
     1981 agagtgcaag gaagcgctgc tcgcctacta cttcctgctg acagggaagg attccgaggg
     2041 aaaggcgtac tcccaggacg agctggacga ccgcatcgag cagtggatga aagaaaagtt
     2101 cggcgtgacg ctggattttg aggtggacga cgcgcttcgc aagctggagg acaagaagtt
     2161 gctggtgaag gaaggcgacc gttacctcgt cccgtcgatc gacgacacgc tggtcaagct
     2221 cgacgagctg tgggac
//
LOCUS       DAOTIL010000442         2232 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminobacteriaceae bacterium isolate AMR_MDS_3026
            NODE_57762_length_2232_cov_3.338539, whole genome shotgun sequence.
ACCESSION   DAOTIL010000442 DAOTIL010000000
VERSION     DAOTIL010000442.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868050
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminobacteriaceae bacterium (wastewater metagenome)
  ORGANISM  Aminobacteriaceae bacterium
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae.
REFERENCE   1  (bases 1 to 2232)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2232)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:19:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,224
            CDSs (total)                      :: 3,198
            Genes (coding)                    :: 3,184
            CDSs (with protein)               :: 3,184
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2232
                     /organism="Aminobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_57762_length_2232_cov_3.338539"
                     /isolate="AMR_MDS_3026"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:3034609"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..957)
                     /locus_tag="P5201_09270"
     CDS             complement(<1..957)
                     /locus_tag="P5201_09270"
                     /inference="COORDINATES: protein motif:HMM:NF015662.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding transpeptidase
                     domain-containing protein"
                     /protein_id="HRV98769.1"
                     /translation="MTRRFGSPWFLVFIFFGVVIWRLTSLQLFPDPRVENQSRRQYWI
                     HVPVSTNRGFIYDRNGNALAVSVPSSSFFLDPAFWNPSDAVHLKGILPDSLVDTLSKP
                     LSGRFLWLARKVDIETAEKIRSLKIKGIFEIEEKKRLYPSGSLLSHVLGFCDIDDKGL
                     SGIELMWDKALFSPPGLRVLAKDSSGRTLDISRNDHDHTPTEGGNVSLTIDSRIQFIV
                     ERRLEEGIREHSARWGSIICMDPQTGAILSMASWPTFNPNVRTDLSKTQNLLNNSIGR
                     TYEPGSTFKPVVLGISMERGSVHPNESFHCPYRIKIADGHISE"
     gene            complement(989..1408)
                     /locus_tag="P5201_09275"
     CDS             complement(989..1408)
                     /locus_tag="P5201_09275"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRV98770.1"
                     /translation="MRFREDIKKETVSDSFHKISPYIIVFTLCAFGLFMGLGVLRLYS
                     FRLECKLNSVNRQIESYKANRIVLKHELSSLVSPARIYGFSKQEMGMTFASNVRILRV
                     EEHLLAEASFESSPVVASKQENEGWFFFFLEGAMAGE"
     gene            complement(1416..>2232)
                     /gene="rsmH"
                     /locus_tag="P5201_09280"
     CDS             complement(1416..>2232)
                     /gene="rsmH"
                     /locus_tag="P5201_09280"
                     /EC_number="2.1.1.199"
                     /inference="COORDINATES: protein motif:HMM:TIGR00006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016434 - rRNA (cytosine)
                     methyltransferase activity [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="16S rRNA (cytosine(1402)-N(4))-methyltransferase
                     RsmH"
                     /protein_id="HRV98771.1"
                     /translation="GLGGYSEAILESFESTEVYAVDQDSHALSFAKERLLPFGARFHP
                     EKGNFASLPPAVTCNAPFDAMLFDLGVSNLQLVDEDRWFSFQSDGPLDMRMDREFRYS
                     AADLVNSYSEREIADIFWKYGEERFSRQLARGIVRYREESGKIERTSTLVDIVRKTLP
                     APVQRKMGGHPARRIFQALRIAVNDEISILETGLHNIPDVAAPGALLVVVSYHSLEDR
                     IVKNLFKKWREEGKGSIVTKRPLVPSDEEIEANHKSRSAKMRVFSFKPKRMEV"
BASE COUNT          618 a          529 c          514 g          571 t
ORIGIN      
        1 ttcggatata tgaccgtcgg caattttgat cctatagggg cagtggaatg attcattcgg
       61 atgaacggag cctctctcca tgcttattcc aagaacaacg ggcttaaacg tagagccggg
      121 ttcatacgta cgcccgatag aattgtttaa taaattctgc gtcttcgata gatccgttcg
      181 cacatttggg ttaaacgtcg gccaactcgc catcgaaaga atggcgcccg tttgaggatc
      241 catacagata atgctgcccc atcgcgcgct atgttcgcga ataccctcct caagacgccg
      301 ctccacgata aactggattc tggagtctat tgtgagggag acattcccac cttccgtagg
      361 tgtgtggtca tggtcatttc tgctgatatc aagagttcgt cccgacgaat ccttggcaag
      421 gacacgaaga cccgggggag aaaacaacgc tttatcccac atgagttcaa tacctgaaag
      481 ccctttgtcg tcgatatcgc aaaatccgag gacatgtgag agaagagagc cgctcggata
      541 gagtcttttt ttctcttcta tctcgaatat acctttaatt ttgagcgagc gaattttctc
      601 agccgtttca atatcgactt ttctcgctaa ccataaaaaa cggccagaaa gaggtttcga
      661 gagcgtatcg actaaactat cggggagtat ccctttgagg tgtactgcat ctgaaggatt
      721 ccaaaaagcg ggatcaagaa aaaaactgga cgaaggaacg gaaaccgcaa gggcgttccc
      781 gttcctgtca taaataaagc ctctattggt actgacagga acatgaatcc agtactgtcg
      841 gcgcgactga ttctcaaccc tcgggtcagg gaagagctga agagatgtca gcctccagat
      901 aacgacgcca aaaaagataa aaactagaaa ccaaggggat ccgaaacgtc gcgtcacaaa
      961 tacaccctcc gttcaggata cacgggaatt actctccggc cattgcgcct tcaagaaaga
     1021 aaaagaacca gccttcattc tcctgtttcg aagcgacaac aggagaactc tcgaaagaag
     1081 cctcagctaa aagatgctct tctacgcgga ggatgcggac attagatgcg aatgtcatac
     1141 ccatctcttg ctttgaaaaa ccgtagatgc gagcagggga aacaagtgat gagagttcat
     1201 gtttaagtac aatacgattt gctttgtatg attctatttg cctgttgaca gaattcagtt
     1261 tgcattccag acggaaagaa taaagacgaa ggactccgag tcccataaag aggccaaacg
     1321 cacagagcgt aaaaacaata atatatggag agattttgtg aaaggaatca gaaacggttt
     1381 ctttttttat atcttcgcga aatcgcatgt cctttttaca cctccattct tttcggtttg
     1441 aatgaaaaaa ccctcatttt ggcgcttcgc gacttatgat tcgcttcaat ctcttcatct
     1501 gaaggaacga gaggccgctt tgtcacaata cttcctttcc cctcttcacg ccatttttta
     1561 aacagattct ttacaatcct gtcttcaaga gaatggtagc tcaccacgac aagaagtgcg
     1621 cccggcgctg cgacatctgg aatattgtgt aaaccggttt cgaggattga gatttcgtcg
     1681 tttactgcta tacgtagggc ttggaaaatt cttcttgccg gatgcccccc catttttcgt
     1741 tggaccgggg ctggcaatgt ttttcgaacg atatctacaa gtgtggacgt gcgttcgatt
     1801 ttgcctgatt cttctctgta tcggacgatt cctcttgcga gctgtcttga aaagcgctcc
     1861 tccccgtact tccagaagat atctgcaatc tctctttctg aataagagtt cacaaggtcg
     1921 gcggcagaat aacgaaactc tcggtccatc cgcatatcca aggggccgtc tgactgaaac
     1981 gagaaccatc tgtcttcatc gacaagctgg aggttcgata ctccaagatc gaacaacatc
     2041 gcatcaaatg gtgcgttgca agtaactgca ggaggaaggg aggcaaaatt ccctttttcg
     2101 gggtgaaaac gggcgccgaa aggaagaagc cgttcttttg caaacgaaag ggcatggctg
     2161 tcctgatcta cggcatatac ctctgtagat tcgaaagact cgagtatcgc ttccgagtag
     2221 cccccgagac ca
//
LOCUS       DAOTIL010000443         2232 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminobacteriaceae bacterium isolate AMR_MDS_3026
            NODE_57773_length_2232_cov_2.547542, whole genome shotgun sequence.
ACCESSION   DAOTIL010000443 DAOTIL010000000
VERSION     DAOTIL010000443.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868050
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminobacteriaceae bacterium (wastewater metagenome)
  ORGANISM  Aminobacteriaceae bacterium
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae.
REFERENCE   1  (bases 1 to 2232)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2232)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:19:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,224
            CDSs (total)                      :: 3,198
            Genes (coding)                    :: 3,184
            CDSs (with protein)               :: 3,184
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2232
                     /organism="Aminobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_57773_length_2232_cov_2.547542"
                     /isolate="AMR_MDS_3026"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:3034609"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1769)
                     /locus_tag="P5201_09285"
     CDS             complement(<1..1769)
                     /locus_tag="P5201_09285"
                     /inference="COORDINATES: protein motif:HMM:NF023400.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ig-like domain-containing protein"
                     /protein_id="HRV98772.1"
                     /translation="MRKFFVALLAIVLLFGGAYGAWAADDFTILSFSPGGMVKGRMPV
                     KIAFSQPVVEKSLVGKTAPQQHIPVEFSPSIRGAGKWTDQKTFVFTPVANLQQATRYR
                     ATAKESLQDAKGRRLSGKQSFEFFTEPLKLLRAQQIDFTESNNVVIELKFNLPVSPFR
                     LRGFLSLLNEQRQQLGYSPAGQAPSSTVRLTTNSFEGGKIALVLSPGLTCDAGPLGIE
                     QEIRHEIAVTRKLEIRSVYADTRYPATSVINVNTSVGADMSVVSKFIEISPATEFTID
                     PGYNGFTILGDFKPKQRVELTIKKGLPTKEGKALEQEFKRAVIFPDYYPSLAFPAGGT
                     FLSPAGDLRIPIESVNIEEITLNLWRLYENNIPVSMTMSNYSIPRDLARRVATRTARP
                     EGDLNETVRRAIDLRELAGEAKGVFLLTASESSGEYWADTEQLVAVTDLGIVARVWAN
                     GITVWVNSILGIEPVKDATVRVYSKSNQLLASGSTDENGLWTKQGAEPWDDQLLPAIV
                     TVEKGDDISFLKLESNLLADTGFDIDGRPWTDKYDALLFAPRGVFRPGENVDFSALVR
                     TKDLLPPEPFPVVYVVRSSLGREV"
BASE COUNT          442 a          637 c          650 g          503 t
ORIGIN      
        1 acttcgcgcc caagagacga acgcacgacg tacaccacgg ggaacggctc cgggggcagc
       61 aggtcttttg tccgcacgag cgcggagaag tccacgttct cgcccgggcg gaagacgccc
      121 ctcggcgcga agagcagcgc gtcgtatttg tcggtccagg ggcgtccgtc gatatcgaag
      181 cccgtatccg ccaaaagatt gctctcaagc ttcaggaatg agatatcgtc tcccttttcg
      241 accgtcacga tggccggaag aagctgatca tcccaaggtt cggcgccctg tttcgtccac
      301 agaccgtttt cgtcggtgga accgctcgca agaagctggt tgctcttcga gtagacgcga
      361 accgtcgcgt ctttgacggg ctctatcccg aggatggagt tcacccacac ggtgatcccg
      421 ttcgcccaga ccctggcgac gatgcccagg tcggtcacgg cgaccagctg ttcagtgtcc
      481 gcccagtatt cgcctgaaga ctcggacgcc gtcagcagga agacgccctt cgcctctccg
      541 gccagttcgc gcaggtcgat ggcgcgccgc acggtttcgt tgaggtcccc ctcggggcgc
      601 gcggtgcgag tggcgactct ccgggcaagg tctctgggta tggagtagtt cgacatcgtc
      661 atggagaccg ggatgttgtt ctcgtacaaa cgccacaggt tgagcgtgat ctcctcgatg
      721 ttcactgatt caatgggaat ccgcagatcg cctgcgggag agaggaaggt ccctccggcc
      781 gggaaggcga gggacgggta gtagtcgggg aagatgaccg ccctcttgaa ctcctgttcg
      841 agcgccttgc cctctttggt gggaagtccc tttttgatgg tcaattccac tcgctgtttg
      901 ggcttgaagt cgccgaggat cgtgaagccg ttgtaccccg ggtcgatggt gaactccgtc
      961 gcgggcgaga tttcgatgaa cttggagacg accgacatat ccgcgcccac ggaggtgttc
     1021 acattgatga cgcttgtcgc gggataccgt gtgtccgcgt agacgctgcg gatctccagc
     1081 tttctggtga ccgcgatctc gtgccggatt tcctgttcta ttcccagagg accggcgtcg
     1141 catgtcagcc cgggagagag aacgagcgca atttttcccc cttcgaagga gtttgttgtc
     1201 aggcggactg ttgatgaagg agcctgtccg gcgggggagt atcccagctg ctgccgctgt
     1261 tcgttcagca gcgagagaaa accgcgcagc ctgaacggcg agacggggag gttgaacttc
     1321 agttcgatca cgacattgtt gctctcggtg aagtcgatct gttgcgccct caggagcttc
     1381 agcggttcgg tgaagaattc gaagctctgc tttccggaga gccgccgccc cttcgcgtcc
     1441 tgcagcgact ccttggcggt cgcacggtat cgcgtcgcct gctggagatt cgccacggga
     1501 gtgaagacga acgtcttctg atccgtccat ttgcccgctc cgcgtatgga cggtgaaaac
     1561 tcaaccggta tatgctgctg cggcgcagtc ttgccgacga gactcttctc cacgactggc
     1621 tgcgagaagg cgatcttcac cggcatcctg cctttgacca ttcctcccgg agaaaacgag
     1681 agaatggtga aatcatctgc cgcccaagca ccgtacgctc cgccgaagag caagacaatc
     1741 gcgagaagcg ccacaaaaaa ctttctcata tgtatcatct cctttttctc tggtctatat
     1801 ggtagtgcgc cgtgcatgtc gttgcttttt gaattatacc tcgaatcgcg gtgaagaggg
     1861 tatgcgaaag agacgaatac gaaatacacg cgatcctcta tagaaaaacg gagagaagac
     1921 attctgtgcc gggctgttgc gttcacccaa aacaatgggt ttctgcaatc gcgcagaccg
     1981 agtacgtcct tctccccgct cttcatccga ggggcttcgg tgttggagcc ttcccctcat
     2041 gcgtatagtg attttttact aagctattcc ctgaatttca ggctcgacaa ccactctttt
     2101 acctgatctt cgtactcctc gaaagagtct gctgttaccg acacgtggat tcggaacagc
     2161 caagaatcag tgaaaaggaa gagatcgacg accttgcggg tatcctcgtt ttctccagtc
     2221 gtgaactcgg cc
//
LOCUS       DAOTIL010000444         2231 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminobacteriaceae bacterium isolate AMR_MDS_3026
            NODE_57796_length_2231_cov_3.432904, whole genome shotgun sequence.
ACCESSION   DAOTIL010000444 DAOTIL010000000
VERSION     DAOTIL010000444.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868050
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminobacteriaceae bacterium (wastewater metagenome)
  ORGANISM  Aminobacteriaceae bacterium
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae.
REFERENCE   1  (bases 1 to 2231)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2231)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:19:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,224
            CDSs (total)                      :: 3,198
            Genes (coding)                    :: 3,184
            CDSs (with protein)               :: 3,184
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2231
                     /organism="Aminobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_57796_length_2231_cov_3.432904"
                     /isolate="AMR_MDS_3026"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:3034609"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            172..720
                     /locus_tag="P5201_09290"
     CDS             172..720
                     /locus_tag="P5201_09290"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRV98773.1"
                     /translation="MREKNRLGLFAGIALLLLLAFSSAALAKPDWVTVKYTGTQLRKQ
                     KNTKIGDGNHLLLVHKFDIINNSKNGDIITAIFDRKIRWNGVFTIVQMTSTTQYFFDG
                     KNSTTWKPAHWNVKFGMTGTNPYKGEWYPGQVYKYEHTIPLNKLIKPYDGSWKTTNEA
                     AFKKFNFKFDKWSLDFQVRSHR"
     gene            832..2025
                     /locus_tag="P5201_09295"
     CDS             832..2025
                     /locus_tag="P5201_09295"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRV98774.1"
                     /translation="MKKTPLLCASALLFVLTCTLPAFATEEQWTTYVNERFGYSVEYA
                     DIYTERDEPENGDGVWLSASEGKYALTLSGGYNVLEEDGERRLRSRLENVAHIVPGSE
                     ESGPDWYRVIYSDDGGKDGNERLFHEYGSINANNWASFILVYPVEERERFAPIVACLE
                     ETLMIAPEDAGGKELRTDAFALVNGRVQKDGTPLDCEVYDIPSGLDNGIANWAVIGTG
                     TSDAVTEKETGVWFFGSEGEFMTFIPLDNENEYQDLLWSPAGDRLILVRGSGARPDMF
                     FDVYAEGMEKQAEFSGLREEIAWLDDGMRIVFTRIDGIREGGAFPGLSYGLQLSVILY
                     DSAVEETIPLKEASVTKNFSFVSVSEDGEKITILETSVRSPKDWAGEEKINEREISVP
                     VPAAG"
BASE COUNT          605 a          545 c          629 g          452 t
ORIGIN      
        1 gacgtcggaa aaggactggg gcgacgagga aaaaatcaca acgcgggaaa taaaagtaga
       61 gcttcccgcg gcagggtaaa gcgtaacgga acgcctgttt taaggcgtat ttgctgcggg
      121 agaggagttt ccgcagcaaa tacgattttt tataaaaatg gggggaaata aatgagagag
      181 aagaacaggc tcggactgtt tgcgggcata gcgttgctgc tgttgctggc attctcttcc
      241 gccgctctgg caaaacccga ctgggtaacg gtcaagtaca cgggaacgca actgcggaag
      301 caaaagaaca cgaaaatcgg ggacgggaat cacctgcttc tggtgcacaa attcgacatc
      361 atcaacaaca gcaaaaacgg cgacatcatc acggcgattt ttgatcgaaa aattcgctgg
      421 aacggtgttt tcacgatcgt gcaaatgacg agtactacgc agtatttttt tgacggcaag
      481 aattctacaa catggaaacc ggcacactgg aacgtaaagt tcggcatgac cgggacgaat
      541 ccctacaaag gcgaatggta ccccggacag gtgtataaat acgaacatac gatacctctc
      601 aataaactta tcaagccgta tgacggcagc tggaagacga ccaacgaagc ggcgttcaaa
      661 aaattcaatt tcaagttcga caaatggagt ctcgattttc aggttcgaag ccataggtag
      721 ttttgaggtt gtatcaaaag agcttcttcc gctgcgctct tcgccgagaa aaccagtttc
      781 gctggaaagg atgctcccgg gcgtctcaat aatcgagagg agaaatgaat cttgaaaaaa
      841 acacccttgt tatgcgcttc ggcgctgctc tttgttttga cgtgcacgct ccccgctttt
      901 gcaaccgagg aacagtggac aacctacgtc aacgagcgct tcggctactc tgtggaatac
      961 gccgatatct acacggaaag agatgagccg gaaaacggag acggcgtctg gctgagcgct
     1021 tcggaaggga agtacgcgct gacgctctcg ggaggctaca acgtccttga agaagacgga
     1081 gaacgcaggc tgcggagcag actcgaaaac gtggcgcata tcgtgcccgg ctccgaagaa
     1141 agcggtcccg actggtacag agtgatctac agcgacgacg gcgggaagga cgggaacgaa
     1201 cgcctcttcc acgaatacgg aagtatcaat gcgaacaact gggcttcgtt catcctcgtc
     1261 taccccgttg aagagcggga acgtttcgcc cccatagtcg cgtgcctgga ggaaacgttg
     1321 atgatcgcac cggaggatgc cggcggcaag gaactccgga cagacgcttt tgctctcgta
     1381 aacggccgcg tgcagaaaga cggaacgcct cttgactgcg aagtgtacga tattccatcc
     1441 ggactggaca acgggatcgc gaactgggcc gtaatcggga cgggaacatc cgacgcagta
     1501 acggaaaaag aaacgggcgt ctggtttttc ggatccgaag gagaatttat gaccttcatc
     1561 cctctggaca acgaaaacga ataccaggat cttctttgga gccctgcggg cgatcgcctg
     1621 atcctcgtcc ggggaagcgg agcaagaccc gacatgttct tcgacgtgta cgcggagggc
     1681 atggagaaac aagccgagtt cagcggcctg cgcgaagaaa tcgcgtggct ggacgacgga
     1741 atgcgcatcg tctttaccag gatcgacggt attcgcgagg ggggtgcgtt tcccggcctt
     1801 tcctacggtc tgcaactgtc cgttatcctg tatgattcgg ccgtggagga aacgatacct
     1861 ttgaaagagg cttccgtcac aaaaaacttc tcgtttgtat ctgtttcgga agacggggaa
     1921 aagatcacga tcctggaaac ttcggtccgc tctcccaaag actgggccgg cgaagaaaaa
     1981 attaacgaac gggaaatatc cgttcccgtg cctgccgcgg gataaagagt cccgcgattt
     2041 ctccgcgttg acgccgtgac gcacataaac agcgagcggt attcagaagg ctgtgcacat
     2101 tcccggcgtt tgctgtaaac tttttggaga cgctccgaca ttatagccgg agcgtctcca
     2161 aaaagttttg cattttacta tcgggatgaa cggagagaga cgccatgcga gagacagaaa
     2221 accgattacc c
//
LOCUS       DAOTIL010000445         2225 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminobacteriaceae bacterium isolate AMR_MDS_3026
            NODE_58009_length_2225_cov_3.430876, whole genome shotgun sequence.
ACCESSION   DAOTIL010000445 DAOTIL010000000
VERSION     DAOTIL010000445.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868050
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminobacteriaceae bacterium (wastewater metagenome)
  ORGANISM  Aminobacteriaceae bacterium
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae.
REFERENCE   1  (bases 1 to 2225)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2225)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:19:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,224
            CDSs (total)                      :: 3,198
            Genes (coding)                    :: 3,184
            CDSs (with protein)               :: 3,184
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2225
                     /organism="Aminobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_58009_length_2225_cov_3.430876"
                     /isolate="AMR_MDS_3026"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:3034609"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            268..1671
                     /gene="nhaC"
                     /locus_tag="P5201_09300"
     CDS             268..1671
                     /gene="nhaC"
                     /locus_tag="P5201_09300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014806954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015385 - sodium:proton antiporter
                     activity [Evidence IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter NhaC"
                     /protein_id="HRV98775.1"
                     /translation="MEERKAREISLRGALMIFGAILAIIVVGRLIFTFDVALLLMFIG
                     MFTTFTYVYVYKFTWRDLFEGGVVPMIARASGAIMILLTVGPLIASWMLAGTIPYLIY
                     TGLQILSPNTFLMAAFVICALSSTMTGTSWGTAATFGVAFMGIAQGLGVPLPAAAGAV
                     VAGAYFGDKLSPVSDTTVLAAAVAEVDVVDHIRSMLWTTVPAFVISLGIYGYVGSQAS
                     GEIDLSSVNEIIAGISKTFKMTPLVLVPPLAVLVLAYRRFPTLPVLWIAVALAVPLAM
                     MQGYDLKEIMRAMAAGPKIVTEVASLDKLLSRGGLAFMTGSVVVVFFAYIFAGQLEYT
                     GTFRRICTSLKEGFIGNSKGRFVLSASLSGILTGLGTGNSYLSEIVPGTMYKDLADEM
                     NISRRVLSRTLEDSGTVVVPMIPWSAAGIYMSSVLGVSVVEYVPWAFLCWLGFLTAWF
                     YGFTGIAIWPSDKKADA"
     gene            1781..>2225
                     /locus_tag="P5201_09305"
     CDS             1781..>2225
                     /locus_tag="P5201_09305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005660028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-aspartyl-peptidase"
                     /protein_id="HRV98776.1"
                     /translation="MLLIRNADLYAPERLGPGDILIAGERVLAVRPAGEIDSTAAASF
                     SDLRVIDAGGCIVAPGIIDRHVHFNGAGGEGGPAHRTPPLQLSSFIKAGVTSAVGMLG
                     TDGVARALPELLMKARGLEVEGISTWILTGSYSFPSPTLTGSVQSD"
BASE COUNT          384 a          752 c          646 g          443 t
ORIGIN      
        1 cacctcgcaa ttttctcttt tcccttacga agggactcca aaagtgtagc agatatccga
       61 taaagatgca ttcttccgga cgcctttttt aaaaaaactc ttgacaaaac gctcgcgaga
      121 gataatgatt atacaataac gtatccataa aagaatccag aaagggggag tgaaacgccg
      181 agaagagtga aaacagcttc tccctacgtc aagttcgtcc gcatttattt cttatgagcc
      241 actcggcaca aggaggttat cgtacacatg gaagaacgga aagcacgtga aatatcgctc
      301 cggggcgcac ttatgatttt tggcgccatc ctggcgatca tcgtcgtcgg aagactgatc
      361 ttcacattcg acgtcgccct gctcctgatg ttcatcggca tgttcaccac cttcacgtac
      421 gtgtatgtct ataagttcac ctggcgcgac ctcttcgagg ggggcgtcgt tcccatgatc
      481 gcccgcgcgt cgggcgccat catgatcctg ctcaccgtcg gcccgctcat cgcgtcgtgg
      541 atgctcgcgg gaaccatccc ctacctgatc tacacgggac ttcagatcct cagcccgaat
      601 accttcctga tggcggcctt cgtcatctgc gccctctcgt ccacgatgac gggaacctcg
      661 tgggggaccg ccgcaacctt cggcgtcgcc ttcatgggga tcgcgcaggg gctcggcgta
      721 ccccttccgg cggccgccgg cgcggtggtc gcgggcgcct acttcggcga taagctcagc
      781 cccgtctccg acaccacggt tctcgccgcg gcggtcgctg aggtcgacgt ggtggaccac
      841 atccgctcca tgctctggac caccgttccg gcgttcgtca tcagtctcgg catttacggc
      901 tacgttggct cgcaggcctc gggcgagatc gatctcagca gcgtgaacga gatcatcgcc
      961 ggcatctcga agaccttcaa gatgacgccg ctcgtcctcg ttccgccgct tgccgtcctc
     1021 gtcctcgcct accggcgctt cccgaccctc ccggtgctct ggatcgccgt cgcgctcgcc
     1081 gtgcctctgg ccatgatgca ggggtacgac ctgaaggaga tcatgcgcgc gatggccgcc
     1141 ggcccgaaga tcgtcaccga ggtcgccagt ctcgacaagc tcctcagcag gggcggactc
     1201 gccttcatga cgggctcggt ggtcgtggtc ttcttcgcct atatcttcgc ggggcagctt
     1261 gagtacacgg gcaccttccg ccgcatctgc acctccctga aggagggctt catcgggaac
     1321 agcaaggggc gcttcgtcct ctccgcctcg ctctccggca tcctcaccgg actgggcacc
     1381 ggcaactcct acctcagcga gatcgtcccg ggcacgatgt acaaagacct cgccgacgag
     1441 atgaacatct cccgccgcgt gctctcccgt acgctcgagg actccggcac cgtggtcgtc
     1501 ccgatgatcc cctggtcggc ggcgggaatc tacatgtcct ccgtgctcgg cgtctccgtc
     1561 gtggagtacg tcccctgggc cttcctctgc tggctcggct tcctgaccgc ctggttctac
     1621 ggcttcaccg gaatcgcgat ctggccctcc gacaagaagg cggacgcgta gcgcgtctgc
     1681 cgggagggag gtttccttcg gcgccaagcg agggaacctc cctcctctcc gcatcgcaac
     1741 gcgccgccca cgggccggac gcgggaaagg agacacacca atgctcttga tacgcaacgc
     1801 cgatctctac gctcccgagc gcctcggtcc cggcgacatc ctgatcgcgg gggagagggt
     1861 gctcgccgtc cgcccggcgg gggagatcga ttccacggcg gccgcctcgt tcagcgatct
     1921 ccgggtgatc gacgccgggg gctgcatcgt cgcgcccggc atcatcgacc ggcacgttca
     1981 cttcaacggc gcgggcggcg agggaggacc cgcccacagg acgccgccgc tccagctttc
     2041 gtcgttcatc aaggcgggcg tcacctccgc ggtcggcatg ctggggaccg acggcgttgc
     2101 ccgcgcgctc cccgagctgc tgatgaaggc gaggggactg gaagtggaag ggatctccac
     2161 gtggattctc acagggagct actcttttcc gtcgccaacg cttaccggaa gcgtccagag
     2221 cgacc
//
LOCUS       DAOTIL010000446         2216 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminobacteriaceae bacterium isolate AMR_MDS_3026
            NODE_58337_length_2216_cov_3.145303, whole genome shotgun sequence.
ACCESSION   DAOTIL010000446 DAOTIL010000000
VERSION     DAOTIL010000446.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868050
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminobacteriaceae bacterium (wastewater metagenome)
  ORGANISM  Aminobacteriaceae bacterium
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae.
REFERENCE   1  (bases 1 to 2216)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2216)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:19:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,224
            CDSs (total)                      :: 3,198
            Genes (coding)                    :: 3,184
            CDSs (with protein)               :: 3,184
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2216
                     /organism="Aminobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_58337_length_2216_cov_3.145303"
                     /isolate="AMR_MDS_3026"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:3034609"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..269)
                     /locus_tag="P5201_09310"
     CDS             complement(<1..269)
                     /locus_tag="P5201_09310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013047916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoacid:ferredoxin oxidoreductase subunit
                     beta"
                     /protein_id="HRV98777.1"
                     /translation="MSAPEMHTNGNPLLKYMRQDKLPHFFCPGCGCGQVVSAFLRACE
                     SLELDLGTMVGVGGVGCTARIPVYINMDCLHGVHGRTLAWATGIKL"
     gene            complement(266..1411)
                     /locus_tag="P5201_09315"
     CDS             complement(266..1411)
                     /locus_tag="P5201_09315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013047915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoacid:acceptor oxidoreductase subunit alpha"
                     /protein_id="HRV98778.1"
                     /translation="MGLESRVHSGVHFMQGNYAAVEGAVAAGCDFFAGYPITPANEVS
                     EGMARRLPAVGGAFFQGEDELCSIYAITAASLAGAKAMTATASAGYDYMQEGIEYAVA
                     VEAPIVVLDVMRCRGENFATQADIMQVRWAAAGDHEMIVLAPSTVQEMFDFTVRAFNL
                     AEEYRTPVIVISEMSLALMRERLEIPDASTIPVVNRKRTTKHPSEYLPFKAESEFGVP
                     DFAELGTGYRVIHSINPHDERGDIEWEPHAFERLYKRITGKIRENRKKISTTQRFMLE
                     DAEIALIAFGSEVRPCLDAVHAAREKGIKAGVLKLDAPWPIPEEAIAETAAGANAVVT
                     VEMNIGRYAGEVERIVCGKCRTARATKNLGTPHTPDEILSVIEEVRS"
     gene            complement(1576..1737)
                     /locus_tag="P5201_09320"
     CDS             complement(1576..1737)
                     /locus_tag="P5201_09320"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRV98779.1"
                     /translation="MTIGSSEIASDMDVIVIAVSRRRLLLGAGMRSPDLFEPVEKVIG
                     KKMLQYLDL"
     gene            complement(1734..>2216)
                     /locus_tag="P5201_09325"
     CDS             complement(1734..>2216)
                     /locus_tag="P5201_09325"
                     /inference="COORDINATES: protein motif:HMM:NF017812.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF917 family protein"
                     /protein_id="HRV98780.1"
                     /translation="GLSGRTGLRRRRSNPGTYIEGVLRGNVKTVSAMVRQDACIVGGL
                     LAVARNPVKAGYLQENAAPGAIKLAIGLGKAVKAVRSGGGDAVTEAILSVLPGEVLCR
                     GRVDAVDRFTAGGMDSGTAYVGEYSVSFWREYMTVEHGEEERLATFPDLITVVDAETG
                     "
BASE COUNT          421 a          720 c          629 g          446 t
ORIGIN      
        1 agcttgatgc cggtggccca ggcaagcgtt ctgccgtgga cgccgtggag gcagtccatg
       61 ttgatgtata ccggaatgcg cgccgtgcag ccgacgccgc cgacgccgac catcgtcccg
      121 aggtccagtt cgagcgactc gcacgcacgg agaaaggcgc tgaccacctg accgcagccg
      181 cagccggggc agaagaaatg gggcagtttg tcctgccgca tgtacttgag cagcggattg
      241 ccgtttgtat gcatttcggg agcgctcatg accgcacctc ctcgatgacg gagagaattt
      301 catccggagt gtgcggcgtt ccgaggttct tggtcgcccg cgcggtgcgg cactttccgc
      361 acacgatgcg ctccacctct ccggcgtacc tgccgatgtt catttcgacc gtcacaacag
      421 cgtttgcgcc ggccgccgtc tcggcgatcg cttcctcggg gatcggccac ggcgcatcga
      481 gcttcagaac tccggccttg atccctttct cgcgcgcagc atgaacagcg tcgagacagg
      541 ggcgaacctc gctgccgaag gcgatgagag caatctccgc gtcctcgagc atgaaacgct
      601 gcgtggttga gatcttcttt ctgttctccc ggatcttccc ggtaattctt ttatagaggc
      661 gttcgaaggc gtgcggctcc cattcgatat ccccgcgctc gtcgtgcggg ttgatcgagt
      721 gaatgacccg gtaccccgtc cccagctcgg cgaagtcggg cacgccgaac tcgctctccg
      781 ccttgaaggg cagatactcg gaggggtgtt tcgtcgtccg tttgcggttc acgaccggga
      841 tagtcgaagc atccggaatc tcaagccgtt cgcgcatcag cgccagcgac atctccgaga
      901 ttacaatgac cggcgtccgg tactcctcgg cgaggttgaa cgcacggacc gtgaagtcga
      961 acatctcctg gacggtcgac ggcgcgagca cgatcatctc gtgatccccg gccgcagccc
     1021 accgcacctg cataatgtct gcctgcgtgg cgaagttctc gccccggcag cgcatcacgt
     1081 cgagcaccac tatgggcgcc tccaccgcga cggcgtactc gatcccctcc tgcatatagt
     1141 cgtaccccgc gctcgcggtc gccgtcatcg ccttggctcc ggcaagggac gcagcggtga
     1201 ttgcgtaaat tgaacacagt tcgtcctcac cctggaaaaa agcgcctccc acggcgggaa
     1261 gtcgtcttgc cattccctcg gagacttcgt tcgcaggggt aatgggatag cctgcgaaaa
     1321 aatcgcaccc ggcggcaacg gccccttcca ctgcagcgta gttcccctgc atgaaatgga
     1381 cgccggagtg aactcggctc tcaagtccca ttcatacacc tccattgtgt atgcccgcgc
     1441 acagcggggg cagaatgtat catgcacacg agattatcat gcaaaaaatg gaatgtcaaa
     1501 tatattttct tctgctcgct gaaaaaaacc ctcccgtcag ggagggctcg acgaccagcg
     1561 caagcgcttt cggagctaca ggtcgagata ctgcaacatt tttttcccga tgaccttctc
     1621 cacgggctcg aagagatccg gagagcgcat ccccgctcca agaagaagcc gtctcctgga
     1681 aaccgcgatg acgataacat ccatgtccga ggcgatctcc gagctgccga tggtcatccc
     1741 gtctcggcgt cgacgaccgt gatgaggtcc ggaaaggtgg caaggcgctc ttcttcgccg
     1801 tgttccaccg tcatgtactc cctccagaag gagacagaat actctccgac atacgcggtt
     1861 ccggagtcca tgcctcccgc cgtgaaacga tcgaccgcgt cgactctgcc cctgcagagg
     1921 acctcgcccg gaagcacgga gagtattgct tcggtcacgg cgtcgcctcc tccgcttcga
     1981 accgccttaa ccgctttacc tagtccgatg gcgagtttga tcgcccccgg cgcggcgttc
     2041 tcttgcagat acccggcctt caccgggttg cgcgcgactg cgagaagccc gcccacaata
     2101 caggcgtcct gacgcaccat cgccgagacg gttttgacgt tcccgcgaag aacgccttct
     2161 atatatgttc ctgggttgga tcgcctccgg cgaaggcctg tacggcctga tagtcc
//
LOCUS       DAOTIL010000447         2208 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminobacteriaceae bacterium isolate AMR_MDS_3026
            NODE_58600_length_2208_cov_3.647004, whole genome shotgun sequence.
ACCESSION   DAOTIL010000447 DAOTIL010000000
VERSION     DAOTIL010000447.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868050
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminobacteriaceae bacterium (wastewater metagenome)
  ORGANISM  Aminobacteriaceae bacterium
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae.
REFERENCE   1  (bases 1 to 2208)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2208)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:19:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,224
            CDSs (total)                      :: 3,198
            Genes (coding)                    :: 3,184
            CDSs (with protein)               :: 3,184
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2208
                     /organism="Aminobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_58600_length_2208_cov_3.647004"
                     /isolate="AMR_MDS_3026"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:3034609"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..531)
                     /locus_tag="P5201_09330"
     CDS             complement(<1..531)
                     /locus_tag="P5201_09330"
                     /EC_number="2.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013048018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008479 - queuine tRNA-ribosyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA guanosine(34) transglycosylase Tgt"
                     /protein_id="HRV98781.1"
                     /translation="MSAKAPLEFVVDAVCPVTGARAGRVVTQHGEIPTPVFMPVGTQA
                     TVKAMAPFELEEIGAKIILSNTYHLHLRPGGELVRAAGGLHNFMRWNHPILTDSGGFQ
                     VFSLASLGRISDEGVVCESHLDGSTHFMSPEWSMETQLHLGSDIAMCFDQCAPYPCSR
                     EEAEEAVRRTTLWAGRS"
     gene            complement(528..1415)
                     /locus_tag="P5201_09335"
     CDS             complement(528..1415)
                     /locus_tag="P5201_09335"
                     /inference="COORDINATES: protein motif:HMM:TIGR00010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TatD family hydrolase"
                     /protein_id="HRV98782.1"
                     /translation="MKNERDFPQSTAGEERTVPTVPALVDTHCHVHADDYGAETAAVV
                     ERAAQAGVARMLVIGSDIRTSRLAVEAAEQFASSGVFAAVGIHPHEAATEPSCSVEGK
                     GIPEELRGLAAHPRVAAIGETGLDYHYDHSPRDQQRNSFREHIRFSAEAGKPLVIHGR
                     ESYDDLRSILHEERGERMRAVVHCFSGTEDDARDFLEMGYFLSFGGPLTFPKNDALRA
                     LFRELPEERILLETDSPWLTPHPFRGKRNEPMHVRLVYEKAAEIRGVPFASFAAAMES
                     NAARLFGWGTERTLGDRTS"
     gene            complement(1572..>2208)
                     /gene="metG"
                     /locus_tag="P5201_09340"
     CDS             complement(1572..>2208)
                     /gene="metG"
                     /locus_tag="P5201_09340"
                     /EC_number="6.1.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013048016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004825 - methionine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006431 - methionyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="methionine--tRNA ligase subunit beta"
                     /protein_id="HRV98783.1"
                     /translation="KTEELSSVLNTLYDILRLSAMLVAPFIPETAARIWEQLGLDGEP
                     VNMRFDGFSWGDRAPGHTVKKGKVLFPRIDLEEWKKAREAKAPAPAQPASEEPAIVHE
                     ELVGIDSFKAVELRVAQILNVEEIPKSKKLYKLTVDLGYEKRTIVSGIKEFFTPDELL
                     HKKIVVVVNLQPAKLCGVESNGMLLAAGDGKKTTLALLTPDKDIPLGSRVS"
BASE COUNT          366 a          723 c          696 g          423 t
ORIGIN      
        1 agagcggcct gcccagaggg tcgtccgccg cacagcttcc tccgcctcct cgcgcgagca
       61 cgggtacggg gcgcactggt cgaagcacat ggcgatgtcg cttccgaggt gaagctgcgt
      121 ttccatagac cactcgggcg acatgaagtg cgtcgaaccg tcgaggtgcg actcgcacac
      181 cacgccctcg tcggagatcc ggccgagcga ggcgagggaa aagacttgaa accctccgct
      241 gtccgtcagg atgggatggt tccaccgcat gaaattgtgc agccccccgg ccgctcggac
      301 gagttcgccg ccggggcgca ggtgaaggtg gtaggtgttg gagaggatga tcttcgcccc
      361 gatctcctca agttcaaagg gcgccatcgc cttgacggtc gcctgagtgc cgaccggcat
      421 gaagaccggg gttggaatct cgccgtgctg cgtgacgacc cgtccggccc gtgcgccggt
      481 gaccggacag accgcgtcga cgacgaattc gaggggagct ttcgcgctca tgacgttctg
      541 tcccccaatg tccgctccgt accccatccg aagaggcggg cggcgttcga ctccattgcg
      601 gcggcgaaag aggcaaaggg aacgcccctg atctccgcag ccttctcata gaccagcctg
      661 acgtgcatcg gctcgttccg cttgcccctg aacgggtgcg gagtcagcca gggtgagtcg
      721 gtctccagca ggatacgctc ctccggcagt tcacggaaga gcgcgcgcag cgcatcgttt
      781 ttcggaaatg tgagcgggcc gccgaaggag aggaagtacc ccatctccaa aaaatcccgc
      841 gcatcgtcct ccgtcccgga gaagcagtgg acgacggcgc gcatccgctc cccgcgctcc
      901 tcatggagaa tgctgcgcag gtcgtcgtag gactcccgcc cgtggatgac aagcggcttg
      961 cccgcctccg cggagaagcg aatatgctcg cggaagctgt tccgctgctg gtcgcgcggc
     1021 gaatggtcgt aatggtagtc gagtcctgtc tcgccgatcg ccgcaacgcg ggggtgggcg
     1081 gccaatcctc gaagctcctc cgggatcccc tttccctcca cggaacagga gggttccgtc
     1141 gccgcctcgt gcgggtgaat ccccacggcg gcgaacaccc cggaggaagc gaactgttcc
     1201 gcggcctcca cggcaaggcg gctcgtccgt atgtcgcttc cgatgacgag catgcgcgcc
     1261 accccggcct gcgcggcgcg ctccactacc gcggcggttt ccgcgccgta gtcgtccgcg
     1321 tgcacgtggc agtgcgtatc cacgagcgcc ggaacggtgg gtacagtacg ctcttcgccc
     1381 gcggtactct gcgggaaatc tctctcattt ttcatctgct tcatcctcat tttctgttcg
     1441 ttatcacgct gttccgcagt gttcggcgga tacaggaatc agggagcctc cccggatttc
     1501 cggagaggct ccccgtcact gatacctgcc gggactcccc gctgcgggag cggcgtcccg
     1561 gatgtgttct cttagctcac gcggctgccg agagggatat ccttgtcggg ggtaagcagc
     1621 gcgagcgtgg ttttctttcc gtccccggcg gcgagcagca tgccgttgct ctccacgccg
     1681 cagagcttcg cgggctggag attcaccacc acaacgattt tcttgtgcag cagctcgtcc
     1741 ggcgtgaaga actccttgat gccggagacg atggtccgct tctcatatcc gaggtcgacg
     1801 gtcagcttgt agagcttctt cgacttcggt atctcctcca cgttcagaat ctgcgccacg
     1861 cgcagctcga cggccttgaa gctgtcgatc ccaaccagct cctcgtggac gatcgcgggc
     1921 tcctccgaag caggctgcgc gggggccggc gctttcgcct cccgcgcctt cttccattct
     1981 tcaaggtcga tgcgagggaa gaggaccttt cccttcttca ccgtgtggcc gggcgcccgg
     2041 tcgccccacg agaatccgtc gaagcgcatg ttcaccggtt cgccgtcaag tccgagctgc
     2101 tcccagatcc gcgccgcagt ttccggaatg aagggggcca cgagcatggc gctcagacga
     2161 aggatgtcgt agagtgtgtt gagcacggag gagagctctt cggtcttc
//
LOCUS       DAOTIL010000448         2207 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminobacteriaceae bacterium isolate AMR_MDS_3026
            NODE_58647_length_2207_cov_3.507435, whole genome shotgun sequence.
ACCESSION   DAOTIL010000448 DAOTIL010000000
VERSION     DAOTIL010000448.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868050
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminobacteriaceae bacterium (wastewater metagenome)
  ORGANISM  Aminobacteriaceae bacterium
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae.
REFERENCE   1  (bases 1 to 2207)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2207)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:19:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,224
            CDSs (total)                      :: 3,198
            Genes (coding)                    :: 3,184
            CDSs (with protein)               :: 3,184
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2207
                     /organism="Aminobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_58647_length_2207_cov_3.507435"
                     /isolate="AMR_MDS_3026"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:3034609"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..197)
                     /locus_tag="P5201_09345"
     CDS             complement(<1..197)
                     /locus_tag="P5201_09345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005659237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate phosphoribosyltransferase"
                     /protein_id="HRV98784.1"
                     /translation="MYSATHEEILGGCTTDVYFVKTRDVLRKSGKLSTDVTAEIFARK
                     NGVFAGLDEALLLLKGKNLAV"
     gene            complement(300..524)
                     /gene="secG"
                     /locus_tag="P5201_09350"
     CDS             complement(300..524)
                     /gene="secG"
                     /locus_tag="P5201_09350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005659239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecG"
                     /protein_id="HRV98785.1"
                     /translation="MKTFLGIVHILLCIALSGAILLQQRKQGGFSGIFGGGTQADMGG
                     SQWQRFTGLSKVTVILTAIFMVTSIVLVLL"
     gene            complement(570..1169)
                     /gene="rdgB"
                     /locus_tag="P5201_09355"
     CDS             complement(570..1169)
                     /gene="rdgB"
                     /locus_tag="P5201_09355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013047706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016462 - pyrophosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RdgB/HAM1 family non-canonical purine NTP
                     pyrophosphatase"
                     /protein_id="HRV98786.1"
                     /translation="MRAPGLERLLFASGNRGKYVEVIDLFAPLGVDILYGPDVVVLDV
                     EETGSSYGSNAFLKAKAYAAASGMPALADDSGMEVRALNWEPGIFSARVAPTDGERIE
                     TILGRMRGVADRYARYVAAFALYFPSGSCIVTEGVCPGTLGEAPAGDRGFGYDPVFSP
                     SGYDRTFGELPDEVKRKISHRAVAGYRLMDILSGNCVVE"
     gene            complement(1180..1869)
                     /locus_tag="P5201_09360"
     CDS             complement(1180..1869)
                     /locus_tag="P5201_09360"
                     /inference="COORDINATES: protein motif:HMM:NF022112.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GerMN domain-containing protein"
                     /protein_id="HRV98787.1"
                     /translation="MGRKYDDFEDGFEVRRDEAPRSSRRSAANREGGRKAPLFIRLIA
                     WTGVLVFCFAAGYVGTSVALRMLNRQDILQREDVAANRGEAEKILNEAPKEIRLNARK
                     VEFTLYYPKDGTISSEKAEVLSGIMEDDIRQVIGKILSTVSGKGASDVKLLNLFRSGD
                     TLFLNFNAHFLSLLKAQGEQGSALLITSVVRTMNENFSPIVQVRFLVDGKEQKGGAPV
                     DLSVPWRLPRG"
     gene            complement(1891..>2207)
                     /locus_tag="P5201_09365"
     CDS             complement(1891..>2207)
                     /locus_tag="P5201_09365"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012870264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008745 - N-acetylmuramoyl-L-alanine
                     amidase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HRV98788.1"
                     /translation="TKAADKRTQTLLAILGNMQQNAKISESTGFAEYLFNGGKAGGLK
                     MRRVAQAPFYVLRGAAMPAVLVELGFLTDRQEAKLLADKSYQRKMANALAGGIREYLK
                     KN"
BASE COUNT          455 a          691 c          587 g          474 t
ORIGIN      
        1 tccacggcga gattcttccc tttgaggagc aaaagcgcct cgtcaagccc ggcgaagacg
       61 ccgttctttc gtgcgaagat ctccgcggtc acatccgtgg acagttttcc gctcttccgg
      121 agaacgtccc gagtctttac aaaatagacg tcggtcgtgc atccgcccag gatttcctcg
      181 tgtgtggcgg aatacaaacg gcctccttca ggacggaagt cacggacgtc ggaaaaactt
      241 tccatcaccg gacacaccgg acaggattgc cctctgctgc gggattccat gggcttctct
      301 tacagaagaa ccaggacgat cgaagttacc atgaagattg cagtcaaaat cacggtgacc
      361 ttggaaagcc ccgtgaaacg ctgccactga cttccgccca tgtctgcctg ggtacctccg
      421 ccgaaaatac cggagaaacc gccctgcttt ctctgctgca gaaggatggc tccggacaga
      481 gctatgcaga gaagaatgtg gacgatacca agaaacgtct tcaagtcgtg cacctcctaa
      541 aaaaatggga accaaatcca aacgaaaagc tattctacca cacaattccc tgaaaggata
      601 tccatcaatc gatatcctgc cactgcccgg tgagaaattt ttcttttcac ctcgtccggc
      661 aattcgccga aggtccggtc gtacccggag ggcgaaaaga ccgggtcgta gccgaacccg
      721 cgatctcccg cgggagcttc cccgagcgtt ccgggacaga cgccctcggt cacgatgcag
      781 cttccggagg ggaaatagag cgcaaaggcc gccacatacc gcgcatagcg gtccgccacg
      841 ccgcgcatac gtccgagaat cgtttctatt ctctctccgt cagtcggtgc gacgcgggct
      901 gagaaaatac ccggttccca gttcaacgcc ctgacttcca tgccgctgtc gtcggccagc
      961 gcgggcatcc cggacgcagc cgcataggcc ttcgctttga gaaaggcgtt gctcccgtag
     1021 ctgctccccg tttcctcgac gtcaaggacg acgacgtcgg gtccgtagag aatgtcgacg
     1081 ccaagcggcg cgaaaagatc aatcacttcg acgtacttgc ctctgttgcc gcttgcgaag
     1141 aggaggcgtt ccagtccggg agctctcatg tgcgcggcgt catcctctcg ggagacgcca
     1201 cggcacgctg aggtcgacgg gcgcgccgcc cttctgctcc ttgccgtcga cgaggaagcg
     1261 cacttgaacg atcggcgaaa agttttcgtt catcgtccgc actacagagg tgatgaggag
     1321 cgcgctcccc tgctctccct gcgctttgag cagcgaaaga aagtgtgcgt tgaagttgag
     1381 aaagagcgta tcgcccgagc ggaagaggtt gagcagcttg acgtcggagg ctccctttcc
     1441 cgaaaccgtc gacagtatct tccctatcac ctggcggatg tcgtcctcca tgataccgga
     1501 gagcacctcc gccttctcgg acgagatcgt cccgtctttc ggatagtaca gcgtaaactc
     1561 caccttgcgg gcgttcaggc ggatctcttt gggcgcctcg ttcagtattt tttcggcttc
     1621 tccgcggttc gccgctacat cctctctttg gaggatatcc tggcggttga gcatgcgcaa
     1681 cgcgacggag gtgcccacat accccgctgc aaaacagaaa accagtacgc cggtccacgc
     1741 aatcagtcga atgaagagcg gcgccttccg tccgccttct ctgtttgcgg cggatcgcct
     1801 cgatgaacgg ggagcctcat ccctccgcac ttcgaaaccg tcttcgaaat catcatactt
     1861 tctccccatc aaatgatcgt cctccttgga tcagttcttt ttcagatatt cccgtatccc
     1921 gcctgcgaga gcattcgcca tcttccgttg ataggatttg tccgcgagaa gcttcgcctc
     1981 ctgcctgtcc gtcaaaaagc ccagttcgac gagcacggcg ggcatcgccg cccccctgag
     2041 cacgtagaac ggcgcctggg ctacacggcg catcttgagt cctcccgctt ttccgccgtt
     2101 gaagagatac tcggcaaacc cggtgctctc gcttatcttc gcattctgct gcatgtttcc
     2161 gaggatggcc agaagcgtct gggtccgttt gtccgcggcc ttcgtcg
//
LOCUS       DAOTIL010000449         2207 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminobacteriaceae bacterium isolate AMR_MDS_3026
            NODE_58651_length_2207_cov_3.210967, whole genome shotgun sequence.
ACCESSION   DAOTIL010000449 DAOTIL010000000
VERSION     DAOTIL010000449.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868050
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminobacteriaceae bacterium (wastewater metagenome)
  ORGANISM  Aminobacteriaceae bacterium
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae.
REFERENCE   1  (bases 1 to 2207)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2207)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:19:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,224
            CDSs (total)                      :: 3,198
            Genes (coding)                    :: 3,184
            CDSs (with protein)               :: 3,184
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2207
                     /organism="Aminobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_58651_length_2207_cov_3.210967"
                     /isolate="AMR_MDS_3026"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:3034609"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..542)
                     /locus_tag="P5201_09370"
     CDS             complement(<1..542)
                     /locus_tag="P5201_09370"
                     /inference="COORDINATES: protein motif:HMM:NF023128.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HRV98789.1"
                     /translation="MLSYFPKLEKKVWSWCAYDVGNSAFATTIMAAIYPVYFREVASR
                     GLAPNISTAYWAYTASAALFLSAFSSPLLGALADIQGSKKRLLAIFTGIGVLSTGCMA
                     FIGPGDWLLALILMAAGTIGFTISIIFYDALLPSLVPPERVDRVSAQGYAFGYLGGGV
                     LLAINFLFIASRTPPPRYPNA"
     gene            691..1221
                     /locus_tag="P5201_09375"
     CDS             691..1221
                     /locus_tag="P5201_09375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013047814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dCMP deaminase family protein"
                     /protein_id="HRV98790.1"
                     /translation="MSFSRPEWDVYFMMMASVAGTRSTCLRRRVGAVIVRDRQIVSTG
                     YNGAPKGLPHCAEVGCLRTLLSIPSGERHEMCRGSHAEMNAIAQAAAAGVCTAGGQIY
                     CTHEPCSFCTKAIINAGIERVIFLHPYPDTLAREMRSQASIEVTCLSPDFFATAGALL
                     GDLFSPAENARRNETE"
     gene            1218..1802
                     /locus_tag="P5201_09380"
     CDS             1218..1802
                     /locus_tag="P5201_09380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005661274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HDIG domain-containing protein"
                     /protein_id="HRV98791.1"
                     /translation="MNFTRENALDLLKKHNKDEGHIKHALAVESSMKFFAEKMGGDVE
                     KWGMAGLLHDIDWEEMQETPERHTIEGARWLSEEGYPEDIVRAVLAHGWGICSDVKPE
                     NDMEKVLFTIDELTGLVMTAALVRPSRSVQDLEVKSVKKKWKDKAFAKGVDRELIVKG
                     AEMIPMPLDTVIEWVILAMRRIEPDLGLGSAAGA"
     gene            1908..>2207
                     /locus_tag="P5201_09385"
     CDS             1908..>2207
                     /locus_tag="P5201_09385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009201929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HRV98792.1"
                     /translation="MPVRVFLADDHPLTRAGISAYLAQETSVELVGEAEDGETAWKLI
                     SEIRPDVALLDIRMPGEDGVSVARRVKESGLPVAVVMLTSYDAQQYVLASLRAGAR"
BASE COUNT          548 a          518 c          639 g          502 t
ORIGIN      
        1 gcatttggat atctcggagg aggcgtcctg ctcgcaataa acaaaaagtt aatggcgagc
       61 aggacgcctc ctccgagata tccaaatgca tacccttgcg cggatactct atcgacacgt
      121 tctggaggga caagcgaggg gagaagcgcg tcgtaaaaaa taattgatat tgtgaaccct
      181 attgttccag ccgccataag gatcagtgca agaagccaat cgcccggacc gatgaacgcc
      241 atgcatccgg tcgagagtac gccgattcct gtgaaaatcg cgagaaggcg cttttttgaa
      301 ccctggatat ctgcaagggc cccgagcaga ggcgaagaaa atgcgctgag aaagagcgcc
      361 gccgaagccg tatatgccca ataggcggtt gagatattcg gtgcaagccc ccgggacgca
      421 acttcgcgaa aatagaccgg atagatagcc gccattattg tcgtggcaaa cgccgagttt
      481 ccgacatcgt acgcgcacca cgaccacacc tttttttcga gtttcgggaa ataagagagc
      541 atatggaact cctctccatg gctaagatgc ggattgtgat ccgtagagag tatcatatca
      601 tgctttcttc agctacactt ccgtcccttt catcccatag agatgttctg cgagtgttat
      661 aatcgaaaaa acagttcaag ggggcgggtt atgagctttt caagaccgga gtgggatgtc
      721 tatttcatga tgatggcgag cgttgctgga acaaggagca cctgtcttcg ccgtagagtc
      781 ggggcggtca ttgtcagaga cagacagatc gtcagcacag ggtacaacgg tgcgccgaaa
      841 ggtctgccgc actgcgcgga ggtaggatgt ctccgtacct tactctctat cccgtcggga
      901 gaacgccatg aaatgtgcag aggatcccac gcggagatga atgcgatagc tcaggcagca
      961 gctgcaggcg tatgtactgc gggcgggcag atatactgca cgcatgaacc ctgctccttt
     1021 tgtacaaaag cgattattaa cgccggtatt gaacgggtga tttttcttca cccgtaccca
     1081 gatactcttg caagagagat gaggtctcag gcgtctatcg aagtgacatg cctttccccg
     1141 gacttctttg ctaccgccgg ggctcttctt ggggatcttt tttcgccggc ggaaaatgcg
     1201 aggaggaacg aaacagaatg aatttcacac gagaaaatgc actggatctt ttgaaaaaac
     1261 acaacaagga tgaaggtcac atcaagcatg cccttgccgt ggaatcctct atgaaattct
     1321 ttgccgagaa gatgggcgga gatgtagaaa aatggggtat ggcgggtctt ctgcacgaca
     1381 tcgactggga agagatgcag gaaacgccgg aacggcatac cattgagggg gcgcgctggc
     1441 tttctgaaga agggtatccc gaggatatcg tgagagcggt tcttgctcac ggatggggca
     1501 tctgctcgga tgtgaaacct gaaaacgata tggaaaaagt ccttttcacg atcgacgaac
     1561 tcaccggtct tgtgatgacc gcggcattgg ttcgcccaag ccgttcagta caggatcttg
     1621 aggtcaagtc ggtgaagaag aagtggaagg ataaagcctt tgcgaaaggc gtggatcgtg
     1681 aactgatagt caaaggagcc gagatgattc caatgccttt ggataccgtc atagaatggg
     1741 tcatcctggc catgagacgt atcgaaccgg atttggggct cggatcagcg gcgggagcct
     1801 gagatactgt tttttcggcg tctttgccga tgttttcctc ttgttgtctt gataccatgc
     1861 tttacggaca ataaaaactc cctgaccggg aaaaaggagt ggatagtatg cctgtacgag
     1921 tctttcttgc tgacgatcat ccgctgacgc gagccggcat atcggcctat cttgcacagg
     1981 aaacctccgt ggagctggtg ggggaggctg aagacggcga gaccgcgtgg aagctcattt
     2041 cggagatacg tcctgacgtg gcgcttttgg atatcaggat gccgggtgaa gacggggtct
     2101 cagttgcccg gagagtgaaa gagtcggggc ttcctgtcgc ggttgtcatg ctcacttcct
     2161 acgatgccca gcaatacgtt cttgcgtctc ttcgggcggg cgcgaga
//
LOCUS       DAOTIL010000450         2205 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminobacteriaceae bacterium isolate AMR_MDS_3026
            NODE_58706_length_2205_cov_4.607442, whole genome shotgun sequence.
ACCESSION   DAOTIL010000450 DAOTIL010000000
VERSION     DAOTIL010000450.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868050
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminobacteriaceae bacterium (wastewater metagenome)
  ORGANISM  Aminobacteriaceae bacterium
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae.
REFERENCE   1  (bases 1 to 2205)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2205)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:19:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,224
            CDSs (total)                      :: 3,198
            Genes (coding)                    :: 3,184
            CDSs (with protein)               :: 3,184
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2205
                     /organism="Aminobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_58706_length_2205_cov_4.607442"
                     /isolate="AMR_MDS_3026"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:3034609"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(366..1910)
                     /locus_tag="P5201_09390"
     CDS             complement(366..1910)
                     /locus_tag="P5201_09390"
                     /inference="COORDINATES: protein motif:HMM:TIGR01891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004180 - carboxypeptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HRV98793.1"
                     /translation="MRKVCTFTALLLLLALFVSPSFGADIGAMTPAKGTAVQWLDAHS
                     EVGAEVAKYIWHNPELGLGEHKSSTVLQEMLKAAGFKVESGVAGMETAFVASWGEGKP
                     VIGIHAEFDALPGLSQVPGLAEEKMVVEGCPGHGCGHNLFGSYSSMAAIAVKKAMEAN
                     GVKGTIKLYGTPSEETLVGKVFFVKEGVYKDADAVISWHPGTSNAVSYASSLAMDNFK
                     VRFYGKASHAASAPWAGRSALDAVELMNIGMNYMREHVRPESRIMYSIPDGGKAPNVV
                     PPFAEVWYFVRAPQYKTVKEIMDWTKKVAEGAALMTQTRMEFIPITAVWQYLPNQVLA
                     KVGFANAQLIGIPPFTDEDEKIAEPFSKSVKVEKGPFLSREFTQYDYDKPFSWATGGG
                     SIDEANTSWVVPMVRFSGSTLAQGTPGHSWQATAQNALPPAFKAALTVAKYMAATALD
                     LYANPSLLEEALAELKASNEKHGPFVDPAKDVPLPTFQLMHNVDESQVPVQTKDMPMP
                     DFSTLK"
BASE COUNT          438 a          612 c          641 g          514 t
ORIGIN      
        1 gttgctccgc ggcgagactc accggaagac agggaaaaac gttccatgac gcggtagagg
       61 tcgaagctgt tctcctcgtt gtggaatccg tcgccttcat tctccttgac caggtactcc
      121 acgtcaatac atcgttgcct caaggcttcc gcaatcgtat ccgatccggc ctttttcacg
      181 cggggggatg ttggctcccc ggtaacgacg agcagcgccc aagagtaacg accggctccg
      241 caaggttcgc cagtttgctg aacctttgcg ttttccgatc gaagaagcgg cagtgcacac
      301 ggtctgttat gcagcgaaat gcgtgccgga ggaaaagctc cctccggcac atggtgtcaa
      361 cagacttatt tcaaagtgga aaaatcgggc atgggcatgt cttttgtctg aacgggtacc
      421 tgcgactcgt caacattgtg catgagctgg aatgtgggaa ggggcacgtc ttttgccgga
      481 tcaacaaagg ggccgtgttt ctcgttggag gctttcagct cggccagcgc ctcctcaaga
      541 agcgaggggt ttgcataaag gtcgagggcg gtcgcggcca tgtatttcgc cacggtaaga
      601 gctgccttga acgctggggg aagcgcgttc tgcgctgtgg cctgccagga gtgccccgga
      661 gttccctggg cgagagtcga gccgctgaag cgaaccatcg gcaccaccca gcttgtattg
      721 gcctcatcga tggagccacc tccggttgcc caggagaatg gcttgtcgta gtcgtattgg
      781 gtgaactctc tggagaggaa ggggcctttc tccaccttga cgctctttga gaagggttcg
      841 gctatcttct catcttcgtc ggtgaacggg gggatgccga tgagctgcgc gttggcgaat
      901 ccgaccttcg ccagcacctg gttgggcaga tactgccaga cggcggtgat gggaatgaac
      961 tccatcctgg tttgcgtcat gagggcggcg ccttcagcta ctttcttcgt ccagtccatg
     1021 atctccttga cggtcttata ctggggggcg cggacgaagt accagacttc agcgaacgga
     1081 ggcacgacgt tcggagcttt tccgccgtcg ggaatggagt acatgattct cgactcggga
     1141 cggacgtgct cccgcatgta gttcatgccg atgttcatca gttcgaccgc gtcgagcgcg
     1201 cttctgcccg cccatggggc ggaggctgcg tgcgaagctt ttccgtagaa gcggaccttg
     1261 aagttgtcca ttgcgaggga ggatgcgtac gaaacggcgt tcgacgtccc ggggtgccag
     1321 gagatgacgg cgtctgcgtc tttatataca ccttccttca caaagaatac cttgccgacg
     1381 agcgtttctt cggatggagt cccgtagagt ttgatcgttc cttttactcc gttcgcctcc
     1441 atggcttttt tcacggcgat ggccgccatg gagctgtacg agccgaaaag attgtgtccg
     1501 cacccgtgtc ccgggcaccc ttcgaccacc attttctctt cggccagccc ggggacctga
     1561 gagagaccgg ggagcgcgtc gaattccgca tgaatgccga tgaccggttt cccctcgccc
     1621 cagctcgcga cgaacgctgt ctccatgccg gcgacgccgg attcgacctt gaacccggcc
     1681 gctttgagca tctcctgaag gacggtggag ctcttgtgtt cgccgagtcc gagttcgggg
     1741 ttatgccaaa tgtattttgc gacttcggct ccgacttcgg aatgcgcgtc aagccattgg
     1801 acggcggttc ccttcgccgg cgtcatcgcg ccgatatccg cgccaaacga cggggatacg
     1861 aaaagagcaa gaagcagcag cagcgctgtg aatgtacaga cctttcgcat agcgttacct
     1921 ccttttgatc caatactacc cgccggagaa ggatcttccg gcaaacgtct ggaatgcgca
     1981 ttcttcacgc aaggagctct gccgcgccaa ccacctcctt taaataaaga caggaaagca
     2041 aaaaactcca cgaaggtcag ccgttggttc agaataccca taatagtagc ttttgtcaat
     2101 actcttatgg ggcaatctag tgtgaacagc cttctggttg tccatttttg ccgtgttgct
     2161 ataatttgct gaaagacaca tatttgtcgt gcagagcgct ctcct
//
LOCUS       DAOTIL010000451         2205 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminobacteriaceae bacterium isolate AMR_MDS_3026
            NODE_58726_length_2205_cov_2.377674, whole genome shotgun sequence.
ACCESSION   DAOTIL010000451 DAOTIL010000000
VERSION     DAOTIL010000451.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868050
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminobacteriaceae bacterium (wastewater metagenome)
  ORGANISM  Aminobacteriaceae bacterium
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae.
REFERENCE   1  (bases 1 to 2205)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2205)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:19:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,224
            CDSs (total)                      :: 3,198
            Genes (coding)                    :: 3,184
            CDSs (with protein)               :: 3,184
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2205
                     /organism="Aminobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_58726_length_2205_cov_2.377674"
                     /isolate="AMR_MDS_3026"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:3034609"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(107..1456)
                     /locus_tag="P5201_09395"
     CDS             complement(107..1456)
                     /locus_tag="P5201_09395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014807703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HRV98794.1"
                     /translation="MYDLVIRGGSVVTPDGVLSTDVGIFGEKIAALGNALEGREMIDA
                     AGKLVFPGVIDPHVHFALPVGGSVSSDDFYSGSVAAACGGVTSVIDFTVGSSDSSIPD
                     DIRNRLEDAKNAVVDYSFHGEVVGWRPGRVDEMNEAIGLGVKSFKFFTAYGASGRRTD
                     TGPLLQAFQALADMDGVAVVHAEDDSMIETFSGLLSNEEWGRMESVAKVRPDVCEASA
                     VNTVGWLAARTGVQCHIVHLSSALGLEEALYARERGADLTVETCPQYLLLTSDVYKGP
                     EGHLYSAAPALRSSDDNAVLWNALASGEIDFAATDHCPFTRTQKEWKGAFDRLPGGLP
                     GVELLLPLLYSEGVLRGRLPLCELARITSERAARRYGLYPRKGSLLPGSDADIVLFDP
                     EEVWHVQAGALRMNVDFSPYENLELIGKTWQTISRGEIIYSEGRFLGKKGRGVFLHR"
     gene            complement(1679..>2205)
                     /locus_tag="P5201_09400"
     CDS             complement(1679..>2205)
                     /locus_tag="P5201_09400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014163857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ornithine carbamoyltransferase"
                     /protein_id="HRV98795.1"
                     /translation="GKNTKGLKVSVIWAYATTHKKPISVPLTQALLFPRYGMDVTLAY
                     PKGYEMPDWVIEQAKKNALENGGSFRITNDQEEAYRDADVVIPKNWGNWVTNESKAVI
                     DSTLEANKHWKCTADRMSLTDKYSLYMHALPADRVNEVEDSVIDGPHSAIYDEAENRL
                     HTAKAVMALTMGGR"
BASE COUNT          443 a          682 c          612 g          468 t
ORIGIN      
        1 tcggccgcag ttccgatccc tgcgctatct ctatgtcgct cggcacatgc attacttctc
       61 agtcctcctt gataaaatac cccttcatgt tggcgcgggg taacgattaa cggtgcaaaa
      121 acactcctcg tccttttttt cccaggaaac gcccttccga gtagataatt tccccccgtg
      181 aaatagtttg ccacgtcttt ccgatcagtt caaggttttc atacggagag aaatcaacat
      241 tcattcgaag cgccccggcc tgtacgtgcc agacctcttc cgggtcaaag agcacgatgt
      301 cggcgtccga accgggcagc aggctcccct tccgcgggta cagaccgtaa cgcctcgctg
      361 cgcgctccga ggtaatccgc gcaagttcgc acaacggcag cctgcctcga agcacacctt
      421 cggagtacaa cagcggaaga agcagttcca caccgggcag tcctccgggg aggcggtcga
      481 aggccccctt ccactctttc tgcgtccgtg tgaaggggca gtggtccgtg gcggcaaaat
      541 caatctcgcc cgaggcaaga gcgttccaaa gaacagcatt gtcgtctgaa ctcctcagtg
      601 cgggagccgc cgagtacaga tggccctcgg gacctttgta gacgtcggaa gtaagcaaca
      661 ggtattgagg acaggtctcg acagtcagat cggcgcctct ttctctggca tagagcgcct
      721 cctcaagccc aagtgcggag ctgaggtgga ctatatggca ctgcaccccg gttctggccg
      781 caagccagcc gacagtattc acggcggacg cctcgcagac gtcggggcgt actttggcca
      841 cgctctccat acgcccccac tcctcgttcg agagaaggcc ggagaacgtc tcaatcatgc
      901 tgtcgtcctc cgcatggacg acggcgacgc catccatgtc ggccaaagcc tgaaaagcct
      961 ggagcagcgg ccccgtatcc gtacgtcgtc cggaagcgcc gtaggccgta aaaaatttaa
     1021 agctctttac gccgagaccg atcgcttcgt tcatctcatc gactcttccg ggacgccatc
     1081 ccacgacctc tccgtggaac gagtagtcta caacggcgtt ttttgcgtcc tcaagacggt
     1141 tccggatgtc gtcggggata ctgctgtcag aggagccgac ggtgaagtcg ataaccgatg
     1201 ttacccctcc gcacgcagcg gcgacgctcc ctgaatagaa gtcgtcgctt gaaaccgagc
     1261 ccccgacagg cagggcgaag tgtacgtgcg gatcgataac tccgggaaaa acaagcttcc
     1321 ccgccgcgtc gatcatctcc cgcccttcga gcgcgttacc aagtgcggcg atcttctccc
     1381 cgaagatccc gacatcggta gagagcactc cgtcgggggt caccacggag cctcctcgta
     1441 tcacaagatc gtacatgcgt tcgatcctcc agtcatttgc tttccttgat atacggcaca
     1501 gcgaacaaag cggacacatt gcactgagca cacaccgcag cgactacatt ctttagagta
     1561 aaaaactgga aatcggcttt ccattgtgcg gaatcagctt cgtatgagta tagcacagga
     1621 agcagaaacg ccgcgctcga atcccgatga gcgcggcgtt tctgcaagga atgcttttct
     1681 accttccgcc catcgtgagg gccatgacgg ccttcgcggt gtgcaggcga ttctccgcct
     1741 cgtcgtagat ggccgagtgc ggtccgtcga tcacggagtc ctccacctcg ttcaccctgt
     1801 cggcgggaag ggcgtgcatg tagagcgagt atttgtcggt gagcgacatg cggtcggcgg
     1861 tgcacttcca gtgcttattc gcctcgagcg tgctgtcgat aaccgccttg ctctcgttgg
     1921 tgacccagtt gccccagttc ttcggaatga ccacgtcggc gtcgcggtag gcctcttcct
     1981 gatcgttcgt gatgcggaag gagccgccgt tctcgagcgc gttcttcttc gcctgttcga
     2041 taacccagtc gggcatctcg taccccttcg ggtaggcgag ggtgacgtcc attccgtagc
     2101 gggggaagag gagcgcctgg gtcagcggca cggagatggg cttcttgtgc gtcgtcgcgt
     2161 acgcccagat gacggagacc ttgagcccct tcgtattctt gcccc
//
LOCUS       DAOTIL010000452         2204 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminobacteriaceae bacterium isolate AMR_MDS_3026
            NODE_58752_length_2204_cov_3.084691, whole genome shotgun sequence.
ACCESSION   DAOTIL010000452 DAOTIL010000000
VERSION     DAOTIL010000452.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868050
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminobacteriaceae bacterium (wastewater metagenome)
  ORGANISM  Aminobacteriaceae bacterium
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae.
REFERENCE   1  (bases 1 to 2204)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2204)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:19:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,224
            CDSs (total)                      :: 3,198
            Genes (coding)                    :: 3,184
            CDSs (with protein)               :: 3,184
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2204
                     /organism="Aminobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_58752_length_2204_cov_3.084691"
                     /isolate="AMR_MDS_3026"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:3034609"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(78..317)
                     /locus_tag="P5201_09405"
     CDS             complement(78..317)
                     /locus_tag="P5201_09405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013048541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Veg family protein"
                     /protein_id="HRV98796.1"
                     /translation="MAGTIRSIREQVALYKGLTVRYRTAKGRRKVEERQGVILETYPN
                     LFTLYVESQDSKVSFSYAELLTREVELELLSGNRN"
     gene            complement(545..>2204)
                     /locus_tag="P5201_09410"
     CDS             complement(545..>2204)
                     /locus_tag="P5201_09410"
                     /inference="COORDINATES: protein motif:HMM:NF015126.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0001514 - selenocysteine incorporation
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="SelB C-terminal domain-containing protein"
                     /protein_id="HRV98797.1"
                     /translation="DGFVALTKTDAVDEDFLELALEDVRDFLTGTFLEGKPIIPVSAI
                     KGSNIGLLREELGRLVDRIRPRPRKGPFFLPIDRSFPMSGFGTVLTGTAYRGELSAGN
                     EGIVLPSGREGRIRSVQVHGADSKVAWAGQRVAVSLAGVSIDEISRGDVLCAKGIYAP
                     TRCFECELHLLESVPSLKHWQRVRLHIGTSDVTARVSLLESPSIQSGDTVPAQIVTEE
                     DVVCLLDQRFVIRRYSPLETIAGGKVLSPFASKPRGRNARAACIKRIKSLMHAATPAE
                     RLAILIDSAGILHLADAVRLLQETPEEVVRIGSKLRPAGGQTSKQTSSESTGQHPVDE
                     HPIFLQGERKFFLSGRSVARLSSEISVFLQTFHQSHPSQKGALLDEVVLSVLKEFDTR
                     TARSFIDFLVEGGTLVMEEGFIRLSAFTPRDDERFSKDSESLLALCRERGFQPPLLEE
                     APQALGMDEKRFSSLLKGMRETGKIAIVSGFLLSFEIEEKLLELLIKEKEGITLARVR
                     DLTNSSRKFILPLLEYIDAKGYTRRAGEVRVLLPSKLPRRRETV"
BASE COUNT          506 a          606 c          578 g          514 t
ORIGIN      
        1 ccttgaataa ttaacaaaaa gtatgcccct ccacgggcga cgtggcgggg catactttta
       61 acataagcag cagagactta atttctgttt ccggacaaga gctccaactc gacttcgcga
      121 gtcaaaagct ccgcataact gaaggaaacc ttgctgtcct gcgattcgac gtaaagagta
      181 aagagattcg gataggtttc gagaatgacg ccttgacgtt cctcgacttt gcgacgtcct
      241 ttggctgtcc ggtaacgaac cgtcagtccc ttgtacaatg ccacttgttc tcggatcgat
      301 cgaattgttc ctgccataca ggtcactccc agcaactcat cattacaaga atgttatcac
      361 aaatactgaa aaatatcaaa tatcatcagg cccgattgtg acgggactgc aagccttctc
      421 ctgtatggac tatttgcctt ttctccggcg aataccgtag agcgtcacac gagaaaagtc
      481 gcttggcggc atgtcgttcg cagccggtac cgtcctctcc gggtggcgtt ccgcatttca
      541 tccttcagac ggtttctctc cttcgcggga gttttgaggg aagtaaaacc cggacttctc
      601 ccgctctcct cgtgtacccc ttcgcatcga tatactccag caggggaagg atgaactttc
      661 tggagctgtt cgtcagatcc cgaacccttg caagcgtgat gccttctttt tccttaatga
      721 gaagttcgag cagcttctct tctatttcga acgagagaag gaagcccgaa acgatggcta
      781 tcttgcctgt ttctcgcatt cctttgagga gcgaggaaaa acgtttttcg tccatgccaa
      841 gagcctgagg cgcctcttca agaagcggag gctggaatcc acgctccctg cagagcgcaa
      901 gaagcgattc cgagtctttg gaaaagcgtt catcgtcacg gggagtaaaa gcgctcaagc
      961 gaataaaccc ctcctccatg accagtgttc cgccttcaac aaggaaatcg ataaaagagc
     1021 gcgccgtacg cgtatcaaac tccttgagca cgctgagaac gacctcgtcg agcagagcgc
     1081 ctttctgcga ggggtgcgat tggtggaatg tctgtaagaa aacggaaatt tccgaggaga
     1141 gacgagcgac gcttcgcccc gagagaaaga actttctctc tccctggaga aaaatcgggt
     1201 gctcatccac tggatgctgt cccgttgatt cggaagacgt ctgtttggag gtctggccgc
     1261 ctgcgggacg aagctttgaa ccgatacgga ctacctcttc cggggtttct tgaagcaacc
     1321 ggaccgcgtc ggcaagatgg agtatgcctg ccgagtcgat cagaattgca aggcgttcag
     1381 cgggggtcgc agcgtgcata agcgacttga ttcgtttgat acaggcggca cgtgcatttc
     1441 tcccccttgg tttcgaggcg aagggtgaaa ggacttttcc tccagcgatc gtttcgagcg
     1501 gactgtacct gcggatgaca aatcgttgat caagcagaca gactacgtcc tcttccgtga
     1561 ctatctgtgc ggggacagtg tccccgctct gtatcgacgg cgactcgaga agggaaactc
     1621 tggccgtcac gtctgaggtt ccgatatgga gtcgaacgcg ttgccagtgt ttcagcgacg
     1681 gcacgctctc aagcaggtgc agctcgcatt caaaacagcg tgtcggggcg tagatgcctt
     1741 tggcacagag gacgtctccg cgcgatatct catcaatgga aacgcccgcg agacttacgg
     1801 ctactcgttg ccccgcccaa gcaaccttgg aatcggctcc gtgtacctgg acgctgcgga
     1861 ttcttccttc cctgccggac gggagcacga tcccttcgtt tccggcgctc agctctcctc
     1921 tataggcggt gcctgtgagc accgttccaa agcctgacat cgggaatgat ctgtcaatgg
     1981 ggaggaaaaa aggccccttt cgcgggcggg gccgaatcct gtcgacgagg cgccccagct
     2041 cctctctcag aaggccgatg ttgctcccct tgattgccga gactggaata ataggttttc
     2101 cctctaaaaa cgttccggtg agaaaatcgc gcacatcctc caaagcaagt tcaagaaaat
     2161 cctcgtcgac cgcgtcagtt ttcgtgagcg caacaaaacc gtcc
//
LOCUS       DAOTIL010000453         2199 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminobacteriaceae bacterium isolate AMR_MDS_3026
            NODE_58944_length_2199_cov_3.293377, whole genome shotgun sequence.
ACCESSION   DAOTIL010000453 DAOTIL010000000
VERSION     DAOTIL010000453.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868050
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminobacteriaceae bacterium (wastewater metagenome)
  ORGANISM  Aminobacteriaceae bacterium
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae.
REFERENCE   1  (bases 1 to 2199)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2199)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:19:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,224
            CDSs (total)                      :: 3,198
            Genes (coding)                    :: 3,184
            CDSs (with protein)               :: 3,184
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2199
                     /organism="Aminobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_58944_length_2199_cov_3.293377"
                     /isolate="AMR_MDS_3026"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:3034609"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..195)
                     /locus_tag="P5201_09415"
     CDS             complement(<1..195)
                     /locus_tag="P5201_09415"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HRV98798.1"
                     /translation="MNRVLLDIRKLTIDFPPSRKRKAVRALKEFSLRLEAGESVSIVG
                     ESGSGKTTLIRCISGHVTPVS"
     gene            complement(192..986)
                     /locus_tag="P5201_09420"
     CDS             complement(192..986)
                     /locus_tag="P5201_09420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013049155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HRV98799.1"
                     /translation="MISIRNLSITYGRMTSRGRSGETEAVKNISLSVEKGKLFALAGE
                     SGSGKSSVLMAIPGLLPEGTRVSGEILLDGRNLTALSEEELNALRWRRIALIPQGAMN
                     SFTPVLTVGRHIEEVLDVHLGLSKRAARERTQELFVRAGLDGALASRYPHELSGGQKQ
                     RAAIALALACDPDWLLCDEPTTALDVITQKGIVDTLKDLVETKGVGMLLVSHDLPLAA
                     SVCSRIFIMKGGTIVEEGAPREIVAHPKNPHSAALVNALLELEREE"
     gene            complement(983..2167)
                     /locus_tag="P5201_09425"
     CDS             complement(983..2167)
                     /locus_tag="P5201_09425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009201975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HRV98800.1"
                     /translation="MRHLFTRWSFWSLLLLVLTALFGGRLFDAPPGREVAAAFSKPLW
                     IDRNLPPTKQLRLTADSPSLRFDWQYAPPSGLSVRIDPSVGQTGRFVEWRKPGAPEPL
                     RLAPLRGGFEADGRDIAFKRSLGLPPFGDAAASLFAGQGSYELSLSGEGNFEGEVVLK
                     LEGGRWGLLGTDQRGRDVAALFVAGVRVSLIVGIFATLLATFLGTSFGLAAGYLGGWT
                     DSILMRIVDLLLSIPLLPVLMALAALWGKGLWQLVLILSVFSWMGTARTVRALVLTLR
                     DAPYIEGLRGLGAPSGYILRKHLLPETLPVLLATMTLGVPGAILSEAGLSFLGLSDPR
                     VISWGRMLHEAHSFGAFTAGAWWMLLPPGLGIALLCLVFLDLGKFLEENVDPRLRKGV
                     RP"
BASE COUNT          399 a          712 c          741 g          347 t
ORIGIN      
        1 cgagaccggc gtcacgtgtc ccgagatgca gcggatgagc gtcgtcttcc cgctgccgga
       61 ctcgccgacg atcgagacgc tctccccggc ctcaaggcga agagagaact ccttgagcgc
      121 ccgcaccgcc tttcgtttcc gcgagggcgg aaagtctatc gtcagcttcc ggatgtcgag
      181 cagcaccctg ttcactcctc tctctccagt tcaagcagcg cgttcaccag cgccgccgag
      241 tgggggttct tcggatgggc gacgatctcg cggggcgctc cctcctcgac gatcgttccc
      301 cccttcataa tgaagatgcg cgagcagacc gacgcggcaa gggggaggtc gtgcgagacg
      361 aggagcattc cgaccccctt cgtttcgacg aggtctttca acgtgtcgac gattcctttt
      421 tgtgtgatga cgtcgagcgc cgtggtcggc tcgtcgcaga gcagccagtc ggggtcgcac
      481 gcgagcgcga gcgcgatggc ggcccgctgc ttctgccctc ccgaaagctc gtgggggtat
      541 cgggatgcca gggcgccgtc gagtcccgct ctgacgaaca gctcctgcgt ccgttcacgc
      601 gccgctcttt ttgagagccc gaggtggacg tcgagcacct cctcgatgtg ccgcccgacg
      661 gtgagcaccg gcgtgaagga gttcatcgcc ccctggggaa tcagcgcgat gcgcctccag
      721 cggagggcgt tcagctcctc ctcggagagc gccgtcaggt tcctcccgtc cagcagaatt
      781 tcccctgaaa cgcgcgttcc ctccggcagc agccccggaa tcgccatgag cacggagctc
      841 tttccgcttc cggactctcc ggccagcgcg aagagctttc ctttttcgac ggagagcgag
      901 atgttcttca ccgcctcggt ctcgccgctt cgtccgcggg acgtcatccg tccgtacgtg
      961 atcgagaggt tgcgtatgga gatcatgggc ggactccttt ccggagccgg gggtcgacgt
     1021 tctcttcaag gaattttccg aggtcgagaa agaccaggca gagcagggcg atgccgagcc
     1081 cgggagggag cagcatccac cacgcgccgg cggtgaacgc gccgaaggag tgggcttcgt
     1141 ggagcatcct gccccaggag atgaccctcg ggtcggagag cccgaggaag gagagtcccg
     1201 cctcggagag aatggcgccg gggacgccga gcgtcatcgt cgccagcagc accgggagcg
     1261 tctccggaag caggtgtttt cgcaaaatgt accccgaggg cgcgcccagg cccctcagcc
     1321 cctcgatgta cggggcgtcg cggagcgtga ggacgagggc gcggacagtc cgcgcggtcc
     1381 ccatccacga gaagacggag aggatgagga cgagctgcca cagccccttt ccccagagcg
     1441 cggcgagcgc catgagcacc gggagcagcg ggatggagag cagcaggtcg acgatgcgca
     1501 tcaggatgct gtcggtccac cctccgaggt agcctgccgc gagtccgaag gaggtcccga
     1561 ggaaggtggc taacagggtg gcgaagatgc cgacgatgag cgagacccgt acccccgcga
     1621 cgaagagcgc ggcgacgtcc cttccgcgct ggtcggttcc aaggaggccc cagcgccctc
     1681 cttcgagttt gaggacaacc tccccttcga agttgccttc gccggagagg gagagttcat
     1741 agctcccctg tccggcgaaa agggacgcag cggcgtcgcc gaatggggga agtccaaggg
     1801 agcgcttgaa ggcgatgtcg cggccgtccg cctcgaagcc ccccctgaga ggagcgagac
     1861 gcaacggctc cggagcgccc ggcttccgcc attcgacaaa acgcccggtc tgccctaccg
     1921 agggatcgat ccgcacggag agccccgagg gaggagcgta ctgccagtcg aaacgaagcg
     1981 agggggaatc cgccgtgagc cggagctgct tcgtcggcgg caggttacgg tcgatccaga
     2041 gaggcttcga gaaggccgcc gccacctccc gtccgggggg cgcgtcgaag aggcgtcctc
     2101 cgaaaagagc cgtcaaaacc agaagcagca gagaccagaa gctccagcga gtgaaaaggt
     2161 gtcgcatcag cggcgtcccc tttccaggcg caccctggg
//
LOCUS       DAOTIL010000454         2197 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminobacteriaceae bacterium isolate AMR_MDS_3026
            NODE_59029_length_2197_cov_3.482260, whole genome shotgun sequence.
ACCESSION   DAOTIL010000454 DAOTIL010000000
VERSION     DAOTIL010000454.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868050
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminobacteriaceae bacterium (wastewater metagenome)
  ORGANISM  Aminobacteriaceae bacterium
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae.
REFERENCE   1  (bases 1 to 2197)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2197)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:19:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,224
            CDSs (total)                      :: 3,198
            Genes (coding)                    :: 3,184
            CDSs (with protein)               :: 3,184
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2197
                     /organism="Aminobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_59029_length_2197_cov_3.482260"
                     /isolate="AMR_MDS_3026"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:3034609"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..267
                     /locus_tag="P5201_09430"
     CDS             <1..267
                     /locus_tag="P5201_09430"
                     /inference="COORDINATES: protein motif:HMM:NF012927.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRV98801.1"
                     /translation="GVTPTPPPVHPGYQIPFADQIKRECEVPTIAVGLITSLDMIEEA
                     LCNQRADLVALGRELLRNPFFPLNEARKRGIELPWPEQYRRAFS"
     gene            complement(480..1964)
                     /gene="ltrA"
                     /locus_tag="P5201_09435"
     CDS             complement(480..1964)
                     /gene="ltrA"
                     /locus_tag="P5201_09435"
                     /EC_number="2.7.7.49"
                     /inference="COORDINATES: protein motif:HMM:TIGR04416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003964 - RNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0000373 - Group II intron splicing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="group II intron reverse transcriptase/maturase"
                     /protein_id="HRV98802.1"
                     /translation="MEHGRPLRSGVAPRGKEYKAEAEISDTDRRGVGGVHSIDDGADN
                     TTRPREGTLLHPRLHERTGDLLHELSGSEEKARAFQRNLYRKAKREKEFRFYSLYDKI
                     WRRDILEYAWKRCRANGGSPGIDGKSFKAIEEEGVGRLLEEIARELREKTYRPSPVKR
                     VYIPKADGSKRPLGIPTIKDRVVQTACLIVVEPIFEADFEDISYGFRPKRSAHGAIEE
                     IVEHVERGFTAAYDADLTKCFDTIPHDVIMEGLKRRVSDGNVLSLVLKFLRTPVVEPD
                     GPVQGTKNRTGTPQGGVISPLLANIVLDALDKKGAELKKLYNARMVRYADDFVILARY
                     IGDPILKAVEETIAGLGLKINEEKTRIVRLWNGETLNFLGYAMNMAGKSDRVALRPSE
                     KAQKKLKERLREIISRQRLYKGIDGIVKELNPLLTGWKNYFRLTTVKREFHDLDFHII
                     GRFHRVAKKSSQRPSRIFRHGVHATLKFMGLRSLCGDRPVNAFR"
BASE COUNT          342 a          707 c          599 g          549 t
ORIGIN      
        1 ggggtcactc cgacgcctcc ccccgtccat cccgggtatc agatcccctt cgccgatcag
       61 atcaagaggg agtgcgaggt tccgaccatc gcggtcgggc tgatcaccag tctcgacatg
      121 atcgaggagg cgctttgcaa tcaaagggcc gacctcgtcg ccctggggcg cgagctgctc
      181 cgcaacccct tcttcccgct gaacgaggca agaaaacgcg gcatagagct cccctggcct
      241 gagcaataca gaagagcgtt cagctaagaa ccgcgagggg cttcctccat tcggaagccc
      301 ctcgattcat tatttcccta gtagagtaga gagaaggcgc acatcgttta ggtcaagcgc
      361 ttcctacgtg tccgcctttc ggctttgtgg caaagaacgc ccggaccgtg cgacgctcgc
      421 cttctccccc tcgtccaacc gtacgtgcgg ttttcccgca tacggctgcc cgacatcctt
      481 caccgaaaag cgttcacggg cctgtctccg cacaagcttc gcaatcccat gaatttcagg
      541 gtcgcgtgga ctccatgcct gaatatccgg ctcgggcgtt gactgctctt cttcgccact
      601 ctgtggaatc tgccgatgat gtggaagtcc agatcgtgga attcccgttt caccgtggtc
      661 agcctgaagt agttcttcca gccggtcaac agagggttga gttccttcac gattccgtcg
      721 atgcctttgt acagtcgttg tctggagatg atttcccgga gtcgttcctt cagcttcttt
      781 tgcgctttct ccgacgggcg cagggcgacg cggtcgctct tcccggccat gttcatggcg
      841 tagccgagaa agttcagcgt ctccccgttc cagagcctga cgatgcgcgt cttctcctcg
      901 ttgatcttca gcccgaggcc agcgatggtt tcttcgacgg ctttcagtat cggatctccg
      961 atgtagcgcg cgagtatgac gaagtcgtcg gcgtagcgga ccatccgggc gttgtagagc
     1021 ttcttgagtt ccgcgccctt cttgtccagc gcgtcgagca cgatgttggc gagcaggggc
     1081 gatatcacgc ccccctgcgg ggttcccgtg cggttcttcg ttccttgaac cggtccgtcg
     1141 ggttccacca cgggtgtcct gagaaatttc aggacgagag agagcacgtt gccgtccgat
     1201 acgcgtcttt tcaggccttc catgatgacg tcgtgcggga tggtgtcgaa gcatttcgtc
     1261 aggtccgcgt cgtaggcggc cgtgaatccc cgctccacgt gttcgactat ctcctctatg
     1321 gctccgtgag cgctcctctt ggggcggaat ccgtaggaaa tgtcttcgaa gtccgcttcg
     1381 aagatcggct cgacgactat gaggcacgcc gtctgcacca ctctgtcctt gatcgtcggt
     1441 atccccagcg gacgtttgct tccgtccgcc ttcggtatgt acactctctt caccggcgag
     1501 gggcgatagg tcttttcccg gagttcgcgc gctatctctt cgaggagtcg ccccacaccc
     1561 tcctcctcga tggccttgaa gctcttcccg tcgattcccg ggcttcctcc gttcgctctg
     1621 catcgtttcc aggcgtattc cagtatgtcg cgtctccata tcttgtcgta caggctgtag
     1681 aatcgaaact ccttttcccg cttggctttt cggtacaggt tgcgctggaa ggcgcgggcc
     1741 ttttcttccg atcctgatag ctcgtgcaat agatctcccg tcctttcatg caatcgtgga
     1801 tgaagcagcg tcccttctct cgggcgggtt gtgttgtccg cgccgtcgtc gatactatgg
     1861 acgcctccga ctcctctccg gtcggtatcg gaaatttcgg cctccgcctt atattccttc
     1921 cctcggggcg caaccccgga ccggagaggt ctcccgtgtt ccatacgcgc tccttcgatg
     1981 cgtgccgccc ctaatcacca cggccgtccc gatgtctgct tcttcccgtt gcttcgtaca
     2041 tcggtgtcag gcttctccct gttgaggcgg gatcgccgac gacaactgcg ttttcgaggc
     2101 caccacgggt tcgcttgcgc tgcggcccgc atcttcaccc gactcgttca agagcctttt
     2161 ccggcttgct tgacgcttcg ggtcgcccct gcacgcc
//
LOCUS       DAOTIL010000455         2196 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminobacteriaceae bacterium isolate AMR_MDS_3026
            NODE_59068_length_2196_cov_3.828585, whole genome shotgun sequence.
ACCESSION   DAOTIL010000455 DAOTIL010000000
VERSION     DAOTIL010000455.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868050
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminobacteriaceae bacterium (wastewater metagenome)
  ORGANISM  Aminobacteriaceae bacterium
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae.
REFERENCE   1  (bases 1 to 2196)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2196)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:19:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,224
            CDSs (total)                      :: 3,198
            Genes (coding)                    :: 3,184
            CDSs (with protein)               :: 3,184
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2196
                     /organism="Aminobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_59068_length_2196_cov_3.828585"
                     /isolate="AMR_MDS_3026"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:3034609"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(195..971)
                     /locus_tag="P5201_09440"
     CDS             complement(195..971)
                     /locus_tag="P5201_09440"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRV98803.1"
                     /translation="MTKRICVLGVCAAAIFGLVLWTAGNASANERNSFMDAVFSAIES
                     AENLNLSDGNMKIASYEGPVSNKDEKFMGLLRAKLRLAHIFRKLEYPEENLSVLKGYE
                     VVRLMIAAASSGSLNTQGAPFDLGDYIVFQLILPLQLEPLIRELPVYYDSMKSKEDWQ
                     EKFDLILETGDEYGPSLNRGKTFHLAGFPKVNTKKEYTMAIDVEFKDESLYYSEADSS
                     IEGWLYSFWLRRYQEGSLETVKVILDWLNTKLDEVEKAVG"
     gene            1249..1752
                     /locus_tag="P5201_09445"
     CDS             1249..1752
                     /locus_tag="P5201_09445"
                     /inference="COORDINATES: protein motif:HMM:NF019057.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YegP family protein"
                     /protein_id="HRV98804.1"
                     /translation="MGKYAVRESKSKFYFNLKADNGEIILTSEGYNREASCLQGVESV
                     RINAPVAAVEDQTVEGFKKETNPKFEVYTDKAGEFRFRLKARNGQIIGVSEGYKAKAS
                     AIKGIESIRKNSSSDVMKVENAVKEEKAEAPVKAEKAAKEEKPAKAEKPAKVEKAAKA
                     PAKKKKA"
BASE COUNT          537 a          599 c          569 g          491 t
ORIGIN      
        1 tcattcgagt acaacccagc cgaggccgaa aagaccggcg gaaccagcta catcgtgctc
       61 gcctacagac acgctgacgg cgaggaagac gacagtattc ttgcgccttc cccctgaacg
      121 accgcacccc gccttcgacg ggaagacggg gtgcgtgcag tgctgtcgaa gagtttccac
      181 agcgggaacc gttgttagcc caccgctttt tcaacctcgt cgagcttcgt gttcagccag
      241 tccaggatga ccttcaccgt ctccagcgag ccctcctggt agcggcgcag ccagaaggag
      301 tacagccacc cctcgataga ggagtccgcc tccgagtagt agagcgactc gtctttgaac
      361 tccacgtcga tcgccatcgt gtactccttc ttcgtgttca ccttcgggaa ccccgcgaga
      421 tggaaggtct tccctcgatt caaagaaggc ccgtactcgt cccccgtctc gaggatcagg
      481 tcgaattttt cctgccagtc ctcctttgac ttcattgaat cgtagtatac gggcagttcc
      541 ctgatgagag gttcaagctg cagcggaaga atgagctgga aaacaatata atctcccaga
      601 tcgaaaggag cgccttgcgt gttcagcgat ccgctggacg cggcggcgat catcaggcgc
      661 acgacctcgt accccttgag caccgacagg ttttcctccg ggtactccag ctttcgaaag
      721 atgtgggcca gacgaagctt cgcccgcagc agccccatga acttctcgtc cttgttcgag
      781 acgggcccct cgtaacttgc tatcttcatg ttgccatcgc tcaagttcag attttccgcg
      841 ctctcaatag cggagaacac cgcgtccatg aacgagtttc gctcatttgc agatgcgttc
      901 ccagccgtcc agagcacaag accgaaaatc gccgccgcgc acacaccaag aacacagatt
      961 ctctttgtca ttgttccacc agcctttcca atgatagggg cagggtcccc cttgctatat
     1021 cgcgattatt ctatcatgac accctcgcac ggcggaacgg tgggggggcg ctttccaggg
     1081 tggtttttct cgccctttgc agctctgttc cgttctcgat tgtgaaaacc gccgacagaa
     1141 gaagcgcgaa tcggcgtttt ttcgcatatt agctgaaaac tatggttgac ccggctattt
     1201 atacgttcta caataaacaa aattagcaaa tcattgggag gggatcgtat gggtaagtat
     1261 gctgttcgcg agtcaaagtc caagttctat ttcaatctga aagcggacaa cggtgagatc
     1321 atcctgacct ccgaggggta caaccgggaa gccagctgcc ttcagggtgt cgaaagcgtt
     1381 cgcataaacg cccccgtggc tgctgtcgag gaccagactg tcgaaggctt caagaaagag
     1441 accaatccca agtttgaagt gtacaccgat aaagccggag agttccgttt ccgtctgaaa
     1501 gccaggaacg gccagatcat cggcgtctcc gaagggtaca aagcaaaagc gagcgcgatc
     1561 aaaggaatcg agagcatcag gaaaaactcc tcttccgacg tcatgaaagt cgagaatgcc
     1621 gtaaaagagg aaaaagccga ggcgccagta aaagctgaaa aagctgcgaa agaagaaaaa
     1681 ccggcaaagg ctgaaaaacc cgcgaaggtc gaaaaagccg caaaagcacc ggcgaagaag
     1741 aaaaaggcgt aacctgtcgc cgttgacggc gggaacccat accgtgggct cggcgcgcgg
     1801 cgatgttctc tcaaattgtc cttgcactgc tttcttttcg caggagggtg gaggacggga
     1861 gcttcccgtt cttcactctc ctgtgttgtc tggtcattcc cgtgcgttgt tttactcaag
     1921 tgcaactggt cttgtttcta gtaagttttt tattctatta aaaacaggtt gctctttagc
     1981 aagaggcttt gtataatgcg gtatatactc gaaaattttc cagtagcttt ctcaaaaaag
     2041 acacttggcg ccgtacataa gcgcaagatt gcgggcagct ctataagccg gggagaaaag
     2101 gagccggggc aatgaaatat ctacgggagg ttcgcgactc cataaagttc ttcttggagt
     2161 cgcgaacctc ccgtagatat ttcattgccc cggctc
//
LOCUS       DAOTIL010000456         2194 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminobacteriaceae bacterium isolate AMR_MDS_3026
            NODE_59135_length_2194_cov_3.218326, whole genome shotgun sequence.
ACCESSION   DAOTIL010000456 DAOTIL010000000
VERSION     DAOTIL010000456.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868050
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminobacteriaceae bacterium (wastewater metagenome)
  ORGANISM  Aminobacteriaceae bacterium
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae.
REFERENCE   1  (bases 1 to 2194)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2194)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:19:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,224
            CDSs (total)                      :: 3,198
            Genes (coding)                    :: 3,184
            CDSs (with protein)               :: 3,184
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2194
                     /organism="Aminobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_59135_length_2194_cov_3.218326"
                     /isolate="AMR_MDS_3026"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:3034609"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..268
                     /locus_tag="P5201_09450"
     CDS             <1..268
                     /locus_tag="P5201_09450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014807493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="Ni/Fe hydrogenase subunit alpha"
                     /protein_id="HRV98805.1"
                     /translation="DENGVIERVNLIVATGHNNYAMNRGVEQVARDYITGPDVKEGAL
                     NRMEHVIRCYDPCLSCSTHAVGKMPLLLEIRNSKGELVRTVTKE"
     gene            265..762
                     /locus_tag="P5201_09455"
     CDS             265..762
                     /locus_tag="P5201_09455"
                     /inference="COORDINATES: protein motif:HMM:TIGR00072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase maturation protease"
                     /protein_id="HRV98806.1"
                     /translation="MRVFVCGYGNPFRTDDGAGHHLAPLLAGFLESCGQDVELRLEHQ
                     LLPEMAEEFAAFDLLLFADARVPGTAHGEGFLLEEIDPDPVLEGLNIHSVGPEWVLAL
                     AAQIGGKAPPALLLSVEGESFDFGEIPTPSCALRIREAAEAFKRWFRQKYPRSGCSNG
                     AQTGI"
     gene            1423..1746
                     /locus_tag="P5201_09460"
     CDS             1423..1746
                     /locus_tag="P5201_09460"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRV98807.1"
                     /translation="MAFLPERVIASFIGEESGVLGQILASVLGSLTLIPGFIAFPMAK
                     ILVENGAGIAQMAVFVSTLMMVGVATAPLEASFFGWRATILRNGMAYFYSFVVGYAVW
                     LTVAL"
     gene            1761..>2194
                     /locus_tag="P5201_09465"
     CDS             1761..>2194
                     /locus_tag="P5201_09465"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="permease"
                     /protein_id="HRV98808.1"
                     /translation="MKRWRLPLVSLLIVLPFAFWKPEFGKKALFLLGENILVVLGILP
                     PVFILLGLFDVWIPRERVAPHMGEDSGVKGTVLSVLLGACSAGPLYIAFPVAEVMLRK
                     GTSLRNVFVFIGAWSTMRIPMILFEIQNLGAVFGLTRYFVSLV"
BASE COUNT          423 a          577 c          637 g          557 t
ORIGIN      
        1 agatgagaac ggagtgatcg agcgggtgaa tctcatcgtt gccaccgggc ataacaacta
       61 cgccatgaac agaggcgtgg agcaggttgc gagggactac attaccggtc ctgacgtcaa
      121 ggagggggcg ctcaaccgga tggagcatgt tatccgttgc tacgatccct gcctttcatg
      181 ttcaacccat gccgttggca agatgccgct gttgctcgaa atccggaaca gcaagggaga
      241 acttgtgagg acggtcacga aagagtgagg gttttcgtct gcgggtacgg caatccgttt
      301 cgaacggacg acggagcggg gcatcatcta gccccgcttc ttgccggctt tctcgagtct
      361 tgcgggcagg atgtcgagct acggctcgaa caccagcttc ttcccgaaat ggcggaagag
      421 tttgccgcat tcgacctgct tctcttcgcg gatgcgagag ttccgggaac tgcacatggc
      481 gagggatttt tactggaaga gatcgatccc gacccggtgc tagaaggatt gaatatccac
      541 tccgtaggcc ccgaatgggt gctcgcgctc gcggcgcaaa tcggggggaa ggcccctccc
      601 gctcttctgc tgagtgtgga gggggaatcg ttcgatttcg gcgagattcc gacgccaagc
      661 tgcgctcttc gtattcgcga ggcggcggag gcatttaaga gatggttccg gcaaaaatac
      721 cctcggagcg gatgttcaaa tggcgcccaa accggtattt gatttctccc ctcgtttgca
      781 gacggttcag taaagaaaag gggcggcagg ttcctgccgc ccctatatct atcccctgaa
      841 ccaccgaata acccacgata ttcccgcgac gcctgccgcg gcgaggaaca caagaattca
      901 cggaggacaa acaaaaccgc gggagacgtt cttctcttta tccactctta tcaccccctg
      961 ggggtattat atcatagcgg ggcctttatg gggtgcgcgc ttcctctcct gagctcccgt
     1021 tatgaaactg cgggaatcct tattcaagga gctcgcctga tatatccccc gccccccgcg
     1081 agggtttttt attaatcacc caaatcatcc ggacggagca gcttctcttg aagagtgcgg
     1141 aggatgtgcg acgttgtggt cctccggtgc gatgtttgat atgatatcgt atgcatagtg
     1201 tcggcgtgcc ggaacttttt tctctctttt ttatgggaaa caaaaaatga aaggatgatc
     1261 cttctttatg acatggattt tgtatttcct gacgggggga tttctggtgc tctccgtact
     1321 cttcagccgt gaaataccaa gaaagcgctc gtaaaagcgt ggaagagttt tatcaacatc
     1381 gttcccgctt ttcttgcggt gctccttctg gtttcatttt ccgtggcgtt tcttccggag
     1441 cgcgttatcg cctcgttcat tggggaggag agcggcgttc tcggtcagat tctggcttcg
     1501 gttctcgggt cgctgacgct gattccgggg ttcatcgcct tcccgatggc gaagattctc
     1561 gtggaaaacg gcgccggaat cgcgcagatg gccgtgttcg tctctacgct tatgatggtg
     1621 ggagtggcca cggcgccgct cgaggcctcc ttcttcggct ggagagcgac gatcctgcgg
     1681 aacggcatgg cgtatttcta ctcgtttgtc gtcgggtacg cagtgtggct gacggtggca
     1741 ttatgaagag aagacagatc ttgaaacgct ggcgcctgcc gctcgtctct ctgctcatcg
     1801 tgctcccctt cgccttttgg aaacccgagt ttgggaaaaa ggcgctcttc cttctcggag
     1861 agaatattct cgttgttctg ggcattttgc ccccggtctt catccttttg gggcttttcg
     1921 atgtatggat tccgagggag agggtagcgc cgcatatggg cgaagactct ggagtgaagg
     1981 ggacggtgct ctccgtgctc ctcggggcat gctccgcagg ccctctctat attgcctttc
     2041 ccgttgccga agtgatgctg cgaaaaggaa cgagccttcg gaacgtcttc gtcttcatcg
     2101 gcgcatggtc cacgatgcgc atcccgatga ttcttttcga gatccagaat ctcggggcgg
     2161 tcttcggact caccaggtat ttcgtttcgc ttgt
//
LOCUS       DAOTIL010000457         2184 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminobacteriaceae bacterium isolate AMR_MDS_3026
            NODE_59518_length_2184_cov_2.952560, whole genome shotgun sequence.
ACCESSION   DAOTIL010000457 DAOTIL010000000
VERSION     DAOTIL010000457.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868050
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminobacteriaceae bacterium (wastewater metagenome)
  ORGANISM  Aminobacteriaceae bacterium
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae.
REFERENCE   1  (bases 1 to 2184)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2184)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:19:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,224
            CDSs (total)                      :: 3,198
            Genes (coding)                    :: 3,184
            CDSs (with protein)               :: 3,184
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2184
                     /organism="Aminobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_59518_length_2184_cov_2.952560"
                     /isolate="AMR_MDS_3026"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:3034609"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..875
                     /locus_tag="P5201_09470"
     CDS             <1..875
                     /locus_tag="P5201_09470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008522663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF819 family protein"
                     /protein_id="HRV98809.1"
                     /translation="GFVVMFALMKNGFPPESWKTWGALAGSWIGGTANMVAIQGALGI
                     SDSAMGYTLLVDNVNYSIWVILLLATVPYAKFFNKWTKTDTSTIDSVGLMLSEVKENT
                     RTKMETADLALLIGAGLAVSALSAWLASFISPMLMDVFGKSDFLSVSTVTIIIATIFG
                     ILCAMTPMNSIPGSSPVSMTMLYIIICLIASRASFKELTDAPYYLAAGFVILGIHALL
                     MAIIAKIFKIDLFTCAVASLANIGAVAAAPIIAAAYKETLVPIGVLMALMGYVCGTAG
                     GLFVAKILSMMAGA"
     gene            877..1965
                     /locus_tag="P5201_09475"
     CDS             877..1965
                     /locus_tag="P5201_09475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013048344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide epimerase"
                     /protein_id="HRV98810.1"
                     /translation="MKIRNIRLGSLSVPLKKPFKTALRTVSEVTTNVVVVETEEGVCG
                     YGEAPPTAVITGDTNGAIRGAVEERLRPLLSGRETDDLNSLLDIVDTALVHNASAKAA
                     VDIALHDLWGKTLGRPLYELLGGTAREVETDYTISVNAPETMVKDSLEAVADGYSALK
                     IKVGTDFHLDMQRLEEIRAAVGKHVLLRVDANQGWTPKEAIRTIRFLEDKGLDIELVE
                     QPVKAHDFEGLKMVTDSVDTLILADESVFSVRDAFTVLSMRAADLINIKLMKTGGIRN
                     AVAICGMARACGVECMIGAMMETKISVSAACHLASALPVITRADLDPPILCASDPVVG
                     GATYRGSSVVPSGGAGLGIEKINGLVLE"
BASE COUNT          411 a          643 c          680 g          450 t
ORIGIN      
        1 taggcttcgt ggtcatgttc gccctgatga agaacggctt ccctcccgag tcctggaaga
       61 catggggcgc gctcgccgga agctggatcg gcggcaccgc gaacatggtg gccattcagg
      121 gagctctcgg catcagcgac tcggccatgg ggtacacgct gcttgtggat aacgtgaact
      181 actcgatctg ggtcatcctt cttctggcca ccgttcccta tgccaagttc ttcaacaagt
      241 ggacgaagac cgacacctcc acaatcgatt ccgtcggcct catgctctcc gaggtgaagg
      301 aaaacacgag gacgaagatg gagacggccg accttgccct ccttatcggc gccggtcttg
      361 cagtttccgc tctttcggcc tggctagcct ccttcatctc ccccatgctt atggacgtct
      421 tcggcaagag cgacttcctc tcggtgagca ccgtcaccat tatcatcgcg accatcttcg
      481 gtattctctg cgccatgacg ccgatgaaca gcattcccgg gtcgtcccct gtgtccatga
      541 ccatgctcta catcatcatc tgtctcatcg cctccagggc gtccttcaag gagctgaccg
      601 acgcgccgta ctaccttgcc gcggggttcg tcattctcgg aatccatgcg ctgcttatgg
      661 cgatcatcgc gaagattttc aagatagacc tctttacctg cgccgttgcc agtcttgcga
      721 atatcggcgc ggtcgctgcg gcgcccatca ttgcggcggc atataaagag acgctcgtgc
      781 cgatcggcgt gctgatggcg ctcatgggat acgtgtgcgg cactgcgggc ggcctctttg
      841 tcgccaagat cctctccatg atggcggggg cgtagaatga agatacggaa catccgtctc
      901 ggctctctct ccgtaccgct gaagaagccg ttcaagacgg cgcttcggac ggtctcggaa
      961 gtgaccacca atgttgttgt ggtggagacc gaggagggag tctgcggata tggagaagcg
     1021 ccccccacgg cggtgatcac cggcgatacg aacggcgcta tccgcggcgc ggttgaggag
     1081 cggcttcgtc cgttgctctc cgggcgggag acggacgatc tgaacagtct gcttgacatt
     1141 gtcgatacag ctcttgtcca caacgcctcc gcgaaagcgg ccgtggatat cgcgctccac
     1201 gatctctggg ggaagactct gggccgcccg ttgtacgaac tcctcggcgg cacggccagg
     1261 gaagtggaaa cggactatac catcagtgtg aacgcgccgg aaacgatggt gaaagacagc
     1321 ctggaagcgg ttgcggacgg ctactcggcg ctcaagatca aggtggggac ggattttcat
     1381 ctcgatatgc agcgtcttga ggagatcagg gcggctgtgg gaaaacatgt gctgctccgg
     1441 gtggacgcca accaggggtg gactcccaag gaggcgatac gcaccatccg cttccttgag
     1501 gataaggggc tcgacatcga gcttgtggaa cagccggtga aagcgcacga tttcgaaggg
     1561 ctcaaaatgg taacggacag cgtcgatacg ctcatcctcg ccgacgagtc cgtcttctcc
     1621 gtgcgggacg ccttcacggt gctctctatg agggctgcgg acctgatcaa catcaagctg
     1681 atgaagaccg ggggtatccg caatgccgtc gcaatctgcg gcatggcccg cgcctgcggc
     1741 gtggagtgca tgatcggcgc gatgatggag acgaagatca gcgtatcggc ggcgtgccac
     1801 cttgcttcgg cgctgccggt gatcacaagg gcggatctcg atcctccgat tctttgcgcg
     1861 tccgaccctg ttgtgggcgg cgcgacgtac agaggttcgt cggtggtccc ctccggcgga
     1921 gcggggctcg gcatcgagaa gatcaacggt ctggtgttgg agtaatcgga aacgaacaga
     1981 agcggggcgc gccggaattg tccggttcgc cccgcttttg ttattcctgc tgcaagagct
     2041 tggcgacaag ctccctcgcg gtcggggtca ccgcgaggcc gcctcctgcg gtttcgcgca
     2101 gcgattccgg gagggcgcgt ccgacctgcc ccatcgcgag ggagcttgtc gccaagctct
     2161 tgcagcagga ataacaaaag cggg
//
LOCUS       DAOTIL010000458         2174 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminobacteriaceae bacterium isolate AMR_MDS_3026
            NODE_59870_length_2174_cov_4.142520, whole genome shotgun sequence.
ACCESSION   DAOTIL010000458 DAOTIL010000000
VERSION     DAOTIL010000458.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868050
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminobacteriaceae bacterium (wastewater metagenome)
  ORGANISM  Aminobacteriaceae bacterium
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae.
REFERENCE   1  (bases 1 to 2174)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2174)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:19:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,224
            CDSs (total)                      :: 3,198
            Genes (coding)                    :: 3,184
            CDSs (with protein)               :: 3,184
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2174
                     /organism="Aminobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_59870_length_2174_cov_4.142520"
                     /isolate="AMR_MDS_3026"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:3034609"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            5..580
                     /locus_tag="P5201_09480"
     CDS             5..580
                     /locus_tag="P5201_09480"
                     /EC_number="2.1.1.100"
                     /EC_number="2.1.1.334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013048735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isoprenylcysteine carboxylmethyltransferase
                     family protein"
                     /protein_id="HRV98811.1"
                     /translation="MTTRNAVTSIRAAVFKMRGGIWTVLFLAVWFLAEPPGPYRSFLG
                     LVLVALGQMLRFWAAGCITRYRGENVGAARLTTWGPYGIVRNPLYVGNWLIGAGWGVL
                     AGWKAFFLFVLSFWILYCTIIVPYEEEYLSKTFGDAYLRYIGSTGRFFPRSLSADRLR
                     GPFDSSVLWRSERHSFYVTLAGTLLLLFFVR"
     gene            complement(611..1168)
                     /locus_tag="P5201_09485"
     CDS             complement(611..1168)
                     /locus_tag="P5201_09485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013048732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isochorismatase family protein"
                     /protein_id="HRV98812.1"
                     /translation="MALLRLRRDSAHFLMIDVQERLLPAISGHEAIHRNILKMERAAA
                     VMNIPFCFTEQYPKGLGGTAAEILASLPEGALRFEKSTFSCCDEEGFNDIVDRQGRPV
                     TVLFGIETHICVLATVLDLLELNRQVIVAADACGSRLHEHAVLAMDAMRGAGALVLPV
                     ETIIYHMMGRSGTPEFKALLPLFKE"
     gene            1426..2067
                     /locus_tag="P5201_09490"
     CDS             1426..2067
                     /locus_tag="P5201_09490"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRV98813.1"
                     /translation="MTRERKNDGNEQNEIWTVGEILSAMSGSEYPQYIVKIVNQVQQW
                     MDESIRADNPEQDIDPVAALFENVMEMDGEALFNSLEWVDEYADLLPNIKKPFGNEDV
                     VALHIGPADFEDSLRMAIDYAALFNRGVSRRVWLISDTFIIGDVYRYISHIRALNRQG
                     IAFRFLLVTPWGWTEIPLAADSAPSSRIAWKNNRKGGTAPGEDDRKRDDRLTR"
BASE COUNT          476 a          549 c          593 g          556 t
ORIGIN      
        1 gaatttgacg acgaggaacg ccgtgactag tatccgggct gctgttttca agatgcgagg
       61 cggtatctgg acggtgcttt ttctcgccgt ctggttttta gcggagccgc cgggaccgta
      121 tcgttctttt ttgggccttg tccttgtcgc tcttggccag atgctgcgat tctgggctgc
      181 aggctgcatc acccgatacc ggggagagaa tgtcggcgcg gcgaggctta caacgtgggg
      241 gccgtacgga attgtccgga accctctcta cgtaggcaac tggctcatag gcgccgggtg
      301 gggagtactc gccggttgga aggcgttttt cctttttgtc ctctccttct ggattctcta
      361 ctgtacaatc atagttcctt acgaagagga atatctttcg aagacatttg gagatgcgta
      421 tttgcgctat attggcagta ccgggcggtt tttcccccgt tccctctctg cggaccgtct
      481 tcgcgggcct ttcgattcct cagtgctgtg gcgaagcgag cgtcactctt tttatgtaac
      541 gctcgccgga acgcttctat tgctcttctt cgtccgctga cagcgaacgg gtcgtatttt
      601 tgccccgagc ctactccttg aagagaggca gcagcgcctt gaactccggc gtgcctgaac
      661 ggcccatcat atggtagatg atggtttcga ccggaagaac gagcgctccc gccccgcgca
      721 ttgcgtccat tgcaaggact gcgtgttcat gcaaacggct tccgcaggca tccgcagcta
      781 cgataacttg ccgattcagc tccagcagat cgaggactgt cgccaggacg cagatatgcg
      841 tttcaatacc aaaaagaacc gtgactggcc tgccttgccg gtcgactatg tcgttgaacc
      901 cctcttcatc acagcatgaa aaagtgcttt tctcgaatcg cagagcgcct tcgggcagag
      961 acgccaggat ttcagccgcg gtccccccaa gtcccttcgg atattgttct gtaaagcaaa
     1021 aagggatgtt cataactgct gctgcacgtt ccatcttcag tatgttgcga tggattgctt
     1081 cgtgacccga aatagcgggg agcagccgct cctgtacgtc aatcataaga aagtgcgccg
     1141 aatcacgtcg aagacgaaga agagccatga tttcacctcc tgagaatttt cccctacgat
     1201 tttacactaa aaagcatgcg gcggactgtc attgcggtct tttttttctc tttatccaac
     1261 tcttttcagc agtcaatttc tttgacgaat ccggaatgaa ggatcttttt ttgaaaacag
     1321 acgaacgcga aactcttttt tctcacttct tttgtttatt cttcctgttg tgagcttctg
     1381 gagtatgtgc tatgcttcca tcagatcaga aacacggagg atggcatgac acgagaacga
     1441 aagaacgacg gaaacgagca aaacgaaatc tggacagtgg gagagattct ttccgccatg
     1501 tcgggatctg aatatccgca gtatattgta aagatagtca accaggtgca gcagtggatg
     1561 gacgagtcga tcagagccga taatcccgag caggatatcg acccggtcgc cgcccttttt
     1621 gagaatgtga tggaaatgga cggagaggct ctctttaaca gtcttgaatg ggtcgacgaa
     1681 tatgccgatc tccttccgaa tatcaagaaa cctttcggaa acgaggatgt tgtcgcgctt
     1741 catatcggtc cggcggattt cgaggacagt cttcgtatgg ccatagacta cgccgctctt
     1801 ttcaatcgcg gggtgagccg cagagtgtgg ctgatcagcg atacgttcat catcggcgat
     1861 gtataccgtt atatatctca tatacgcgct ttgaatcgtc aggggattgc atttcgcttt
     1921 ctcctggtca ctccctgggg atggaccgaa ataccgcttg cggcggacag cgcgccttca
     1981 agccgtattg catggaagaa caaccgaaag ggaggaacgg ctccgggaga agatgacaga
     2041 aaacgagatg accgtttgac gagatagcct tcgggtgtta caatcttcgc cgttgccggg
     2101 aacgttcggg aacacggtgt cggggcgtag cgcagtctgg taagcgcgcc tggttcggga
     2161 ccaggaggtc ggag
//
LOCUS       DAOTIL010000459         2174 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminobacteriaceae bacterium isolate AMR_MDS_3026
            NODE_59872_length_2174_cov_4.006135, whole genome shotgun sequence.
ACCESSION   DAOTIL010000459 DAOTIL010000000
VERSION     DAOTIL010000459.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868050
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminobacteriaceae bacterium (wastewater metagenome)
  ORGANISM  Aminobacteriaceae bacterium
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae.
REFERENCE   1  (bases 1 to 2174)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2174)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:19:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,224
            CDSs (total)                      :: 3,198
            Genes (coding)                    :: 3,184
            CDSs (with protein)               :: 3,184
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2174
                     /organism="Aminobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_59872_length_2174_cov_4.006135"
                     /isolate="AMR_MDS_3026"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:3034609"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1454
                     /locus_tag="P5201_09495"
     CDS             <1..1454
                     /locus_tag="P5201_09495"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012957.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="choline/carnitine O-acyltransferase"
                     /protein_id="HRV98814.1"
                     /translation="PINSNVFSLLDLPPVTGSSPRARRAAVLIAAALSLKKSIDDETL
                     PPDTERGTPLCMSQYKNMFGSTRIPGISGDELFVAPNSRHIAVFYRGRAWSLEVLSPE
                     GEGASYDSIVARLDEISASVQQGEGIGVGTTLPRRHWGTLREKLFSFCPENRRNMEMI
                     ESALFVLSLDPKNPEPLREKGFHYLAGTGANRWYDKSFQILVDEQGETGLNFEHSALD
                     GSQHARLAAAILKTGERASAGEWKAPPALPLSFLLSEELAHELKEAERSFERLRENTR
                     LRVLEFTSFGREKIKRLGMSPDAFCQTAMQATIFDLYGKFLNAYEAVMTRQFLLGRTE
                     AMRPVTPEIAAFVHARTAEHLKAASSAHTGRIAECREGRGVDRHLFGLKKMMELFGQE
                     IGVEETPALFSSPGYLALSRNLLSTSSGPTECFRIYGFGPVDDEGFGVRYLMFPDKLV
                     FTMTSRTVMEHRLLAFRERLSENLEKIAALLSE"
     gene            complement(1547..>2174)
                     /locus_tag="P5201_09500"
     CDS             complement(1547..>2174)
                     /locus_tag="P5201_09500"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012678.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0030554 - adenyl nucleotide binding
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase"
                     /protein_id="HRV98815.1"
                     /translation="ADPEMTATMPKNLTAFTGMDALVHAIEAYTCRVATPFSDAPAIT
                     AMELIGKYLRRAWKDGGDLEAREGMLLGSLLAGMAFSHSDVAAVHCLAEALGAIYDAP
                     HGACNSIALPAIMEFSMDACVERYARVATCLGLSFSTPEEGAKKAVEEVKQMARDLEL
                     PSISVFGVKEEDFEEIAAKSEANSSNPDNPKILSKADYVHILRKLTTA"
BASE COUNT          429 a          639 c          640 g          466 t
ORIGIN      
        1 tgccgatcaa cagcaatgtg ttttcactgc tcgatcttcc gcccgtaacc ggttcgtcgc
       61 cgcgtgcccg gcgggctgct gttttgatcg cggcggctct ctcgttgaag aagagcatcg
      121 acgacgagac gctccccccg gatacggagc ggggaacgcc tctctgcatg agccagtaca
      181 agaacatgtt cggcagcacg cgtattcccg gcatctcagg agacgaactc ttcgtcgcgc
      241 cgaacagccg ccacatcgca gtcttttaca gggggagagc atggtcgctt gaggttctct
      301 cgcctgaggg ggaaggcgct tcgtacgaca gcattgtcgc ccgactcgac gaaatctcgg
      361 catcggtgca gcagggagag ggtattggcg ttggtacgac tctgccccgc aggcactggg
      421 gaacgcttcg ggagaaactc ttctcattct gcccggagaa ccgcagaaac atggagatga
      481 tcgagtccgc cctttttgtt ctctctcttg atccgaaaaa ccccgagccg cttcgcgaga
      541 aggggttcca ttatctcgcg ggaaccggag caaaccgctg gtatgacaag tcgttccaga
      601 tactcgttga tgaacaggga gagaccggct tgaacttcga gcactccgcc ctggacggtt
      661 cccagcacgc acggcttgct gcggctattc tcaaaacggg agagagggcg agcgcggggg
      721 aatggaaggc tcctccggct cttccgctct cctttcttct ttcggaagaa ctcgctcatg
      781 aactgaaaga ggctgagcgc tcatttgaac gtctgcgtga aaacacaagg cttcgcgtgc
      841 tggaattcac atccttcggg cgcgagaaga tcaaacgtct cggcatgagt ccggacgctt
      901 tttgtcagac ggcgatgcag gcgacgatct tcgatctgta cgggaaattc ctcaacgcgt
      961 atgaagcggt tatgacaagg cagttccttc tgggccgcac cgaggccatg cgtcccgtca
     1021 cgccggagat agcggctttt gtgcatgcac gaacggcgga acacctgaag gcggcttcct
     1081 cggcgcacac cgggcgaatt gccgaatgca gggagggacg cggcgtcgac cgccacctct
     1141 tcgggcttaa aaagatgatg gaactcttcg gacaggagat cggggtggaa gaaacgcctg
     1201 cccttttctc ttccccgggg tacctcgcgc tctcgagaaa tctgctctcc acgagctccg
     1261 gtcctacgga gtgcttccgc atctacggtt tcggacctgt tgacgacgag gggttcggcg
     1321 tcaggtacct gatgttcccc gataaactgg tctttacaat gacaagccgt acggtaatgg
     1381 aacaccgtct gctcgccttc agggagaggc tgtcggagaa ccttgaaaag atagccgctc
     1441 tcctctccga ataaagagga gcgaaggagg cggtcctccc gctcaccctt cgctcctctt
     1501 tttctccatt tatttcacct ttttctcata cattccgcgg taactgctat gccgttgtca
     1561 gcttcctgag tatatggacg taatcggctt tcgagagaat cttcgggttg tcggggtttg
     1621 acgagttcgc ctccgacttc gccgcaatct cctcgaagtc ctcttccttg acgccgaaga
     1681 cggagatcga aggcagttca agatcgcgcg ccatctgctt gacctcctcc accgccttct
     1741 tcgcaccctc ctccggcgtc gagaaggaga gtccgaggca ggtggcgacg cgggcgtagc
     1801 gttccacgca cgcatccatg gagaactcca tgatcgcggg cagagcgatc gagttgcatg
     1861 cgccgtgcgg cgcgtcgtat atagccccga gagcttcagc aaggcagtgg acggctgcca
     1921 cgtcggagtg gctgaaggcc attcccgcaa gaaggctccc caggagcatt ccctcacgcg
     1981 cttccaaatc gccgccgtct ttccaagccc tccgcaggta tttgccgatg agttccatcg
     2041 ccgtgatcgc cggggcgtcg gaaaaaggcg tggcgacgcg gcaggtgtag gcctctattg
     2101 cgtgaacgag agcgtccatg ccggtaaatg cggtcaggtt cttcggcatg gtggccgtca
     2161 tctcggggtc ggcg
//
LOCUS       DAOTIL010000460         2158 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminobacteriaceae bacterium isolate AMR_MDS_3026
            NODE_60483_length_2158_cov_3.142178, whole genome shotgun sequence.
ACCESSION   DAOTIL010000460 DAOTIL010000000
VERSION     DAOTIL010000460.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868050
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminobacteriaceae bacterium (wastewater metagenome)
  ORGANISM  Aminobacteriaceae bacterium
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae.
REFERENCE   1  (bases 1 to 2158)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2158)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:19:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,224
            CDSs (total)                      :: 3,198
            Genes (coding)                    :: 3,184
            CDSs (with protein)               :: 3,184
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2158
                     /organism="Aminobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_60483_length_2158_cov_3.142178"
                     /isolate="AMR_MDS_3026"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:3034609"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..496)
                     /locus_tag="P5201_09505"
     CDS             complement(<1..496)
                     /locus_tag="P5201_09505"
                     /inference="COORDINATES: protein motif:HMM:NF018455.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HRV98816.1"
                     /translation="MAKAESVRYFCSACGASSASWAGRCPSCGEWGSLEKGTAASASG
                     VSTPQKTKSVKATDVLPPERVSSGIEEFDRVIGGGWIPGGVFLLGGQPGIGKSTLLLQ
                     TCGSMAESIGSVLYVSGEESASQIALRARRLGVLSDELHLVCTNDMAAALESLPGKKF
                     LVVDS"
     gene            667..1869
                     /locus_tag="P5201_09510"
     CDS             667..1869
                     /locus_tag="P5201_09510"
                     /inference="COORDINATES: protein motif:HMM:NF024785.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP phosphoribosyltransferase regulatory
                     subunit"
                     /protein_id="HRV98817.1"
                     /translation="MNRLPRGCANVGGDLAAAMEECKRVFLSLFEQWGYRPFLPSGLQ
                     LLESSWERLPSLFRQKLIAVNSPYGEPCCLRGDITLATVAYLASHHSPEERPLRICYS
                     DRVYARAEKPRLAMESFQIGAELLGWERQGADAEMLFLLLRMLDEIGLEKSILLLGDA
                     TFLAKAVSEIEFSAAERIVGALQKGSLIEYAALLDEENIPDFYRAILQRIPELHGGPE
                     VLEEAAALWGRGAPLLSLKNVVSTLTALGYGDRIAIDLSLVRELGYYSGPIFDVYSEN
                     YGQSLGGGGRYDRLLSLCGLQGQAMGFGLDLQHIASLSKFRTRSPSVMAWFANLSPDE
                     ALRRAERLATGGRPLEMSWCVDRASSLENAAQRGCSSWIDLSDGSTLDLATQGDGRTL
                     PGKENFRC"
     gene            1863..>2158
                     /locus_tag="P5201_09515"
     CDS             1863..>2158
                     /locus_tag="P5201_09515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005661817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP phosphoribosyltransferase"
                     /protein_id="HRV98818.1"
                     /translation="MLTIALPTGRVFSEAAELLMAAGLPASSLASPGRKLVFEEENFR
                     YILAKPSDVPLYVSYGAADLAFAGGDVLQESALPLVEILDTGRGRCRVVVAGPAS"
BASE COUNT          406 a          698 c          644 g          410 t
ORIGIN      
        1 cgctgtccac cacaaggaac ttcttcccgg ggagcgattc gagcgccgct gccatatcat
       61 tggtgcagac gagatgcagc tcgtcggaga gcacccccag ccgcctggcc cggagcgcga
      121 tctgcgaggc cgattcctcg ccggagacgt agaggacgga accgatactc tccgccattg
      181 agccgcacgt ctggagcagc agcgtcgatt ttccgatgcc gggctgtccg ccgagcaaga
      241 agacgccccc cggaatccat ccgccgccga tgacgcgatc gaactcctcg atcccggaag
      301 aaacacgctc cggcggaagc acatccgtag ccttgacgct cttggtcttc tgcggtgtac
      361 tcacgccgga tgctgaagcg gcagttcctt tttcaagcga cccccactcg ccgcaggagg
      421 gacatcttcc cgcccacgaa gcggaggacg ccccgcaggc agagcagaaa taccgtacgg
      481 attcagcctt cgccatacct ctcgcctccc agctcagtgt atctctcccg ctttctcctg
      541 tcgagaggct ttacactttt acaatacaat gatatcatgc cgtgagtatt aaagacatta
      601 aggtgataat ctacccagct aaagtgagga aataacaatg acaatacaaa tcaagagggt
      661 acacatatga accgccttcc gcgcggctgc gccaacgtcg ggggggatct tgcggcggca
      721 atggaagagt gcaaacgggt ctttctttcg ctcttcgagc aatggggcta ccgcccgttc
      781 ctgccttcgg gtcttcagct tctcgaatct tcatgggagc ggctgccgtc gctcttccga
      841 caaaaactca tcgcggtgaa ctccccgtac ggcgagccgt gctgccttcg cggagacatc
      901 acgctggcga ccgtcgcgta cctcgcgtcg caccactcgc ccgaggagcg tccgctgcga
      961 atctgctact cggacagggt gtacgcgcgc gcggagaaac cccgccttgc gatggagtcg
     1021 ttccagatcg gcgcggagct gctcgggtgg gaacggcagg gagcggacgc ggagatgctc
     1081 tttcttcttc tccgaatgct cgacgaaatc ggactggaga agagcattct cctgctgggc
     1141 gacgcaacgt ttctggcaaa ggcggtctcc gagatcgaat tttcagccgc cgagaggatc
     1201 gtcggcgccc ttcagaaagg atcgctgata gaatacgccg ccctcctcga cgaggagaac
     1261 atccccgact tctacagggc tattctccaa cgtattcccg agcttcacgg cggtccggag
     1321 gtgctcgaag aagcggcggc gctctgggga agaggcgctc ctctcctctc gctcaagaac
     1381 gtcgtctcca cgctgacggc gctcggctac ggcgaccgta tcgccatcga cctgtcgctc
     1441 gtccgcgaac tcggctacta cagcggaccg atcttcgacg tctattccga aaactacgga
     1501 cagtctctcg gaggcggagg gaggtacgac cggctgctct cgctctgcgg actccagggg
     1561 caggcgatgg ggttcggact ggatctccag catatcgcgt cgctctcaaa gtttcgcacg
     1621 cgttcccctt ccgtcatggc ctggttcgcg aatctctcgc cggacgaggc gttgcggcgg
     1681 gctgaacgcc tggcgacggg agggaggccg ctcgaaatga gctggtgtgt cgaccgggct
     1741 tcctccctcg aaaatgccgc gcagcggggg tgctcctcct ggatcgatct ctcggacggc
     1801 tcgacgcttg atcttgcaac gcaaggggat ggccggacgc tccccggaaa ggagaatttc
     1861 cgatgctgac catcgccctg cccacaggcc gcgtcttctc cgaagcggcg gagctgctca
     1921 tggccgccgg actgccggcg tcatcccttg cctcgccggg acgcaagctc gtctttgaag
     1981 aggagaactt ccgctacatc ctggcgaaac cctcggacgt gccgctctac gtgagctacg
     2041 gggcagccga cctcgccttc gcaggaggcg acgttctcca ggagagcgcg ctgccgctcg
     2101 ttgaaatcct cgacacgggg agagggcgct gccgcgtcgt cgtcgccggg cccgcctc
//
LOCUS       DAOTIL010000461         2157 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminobacteriaceae bacterium isolate AMR_MDS_3026
            NODE_60516_length_2157_cov_3.656518, whole genome shotgun sequence.
ACCESSION   DAOTIL010000461 DAOTIL010000000
VERSION     DAOTIL010000461.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868050
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminobacteriaceae bacterium (wastewater metagenome)
  ORGANISM  Aminobacteriaceae bacterium
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae.
REFERENCE   1  (bases 1 to 2157)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2157)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:19:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,224
            CDSs (total)                      :: 3,198
            Genes (coding)                    :: 3,184
            CDSs (with protein)               :: 3,184
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2157
                     /organism="Aminobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_60516_length_2157_cov_3.656518"
                     /isolate="AMR_MDS_3026"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:3034609"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            324..>2157
                     /locus_tag="P5201_09520"
     CDS             324..>2157
                     /locus_tag="P5201_09520"
                     /inference="COORDINATES: protein motif:HMM:NF014763.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HRV98819.1"
                     /translation="MNRIFNTVRGRILVSFLAMGLLVLGGVSYVAQRQSAAALLASAE
                     DEGIALVRTLQEGFEQYLTARTAFMEAAAGHAEMKSGDWERQRAFLSSLDAKGMQIQA
                     YFLIDLQGDGHYLDGKVNKLGDREYFQRARDTRKTVVGQPVLSRATGETVVIVASPVF
                     SEKGELLSVLCGRITANTLITLASTQKWGESGHALVTDANGLFIVHPDKEFIGAVNAT
                     QAGEKIPEALAAAAKRALGGEQGIARYVEGGAEQVAAYSRVPSTGWSVAMAASVDEFL
                     APVRAMRNVILTSLLVAALLVIAVSFWIAASVSRPLARVVAKMDELATGDFSSSFEPR
                     SSLVEMRRLSSSFNGMIASVADFAGGVFGVSRKLLARAEDMSAASEQSSASVHEVIGL
                     VGKVAQNSHDAASAIEEANAGVGEVAHGAQAGAKAAVETGERAQEISTAAETGGSALE
                     EMVHLIGEVSRSGASVNAAVNNLASSVSGITGFVNTITQIADQTNLLALNAAIEAARA
                     GEAGRGFAVVAEEVRKLAEESNRAASEVGRVIGEISQKTEHALADQKGSVEQIRQLVV
                     RAKETKAVIDDVVLKVGAITENVQSIAATMQEQSASAEEMTAGMD"
BASE COUNT          428 a          609 c          737 g          383 t
ORIGIN      
        1 gaagtagttt ataaaaagcg gtatttgctg cgggaaaaac gacggaagag ggcgcgacgc
       61 tctcttccgt cgtttttggt aaaaagtctt tcgttcaacg cagagcttca ataggcatct
      121 gtgtataaaa caggttcgca agtcctttac aaaagtccat acatgtgata gttttacaag
      181 gaaatggaat gtgaatatta tatggagaat atctattaca gggtgtacac cttgcgaaaa
      241 gttccgaaaa atcagcgagg tgttttaatg ttttttgtgt tccgtttcaa acacggcccg
      301 agggccgata aggaggagag ggtatgaaca ggattttcaa cacggtgcgc ggacggattc
      361 tggtatcgtt tctcgcgatg gggctgctgg ttctgggcgg agtgagctac gtcgcgcaac
      421 gtcagtcggc ggcggcgctt cttgcatccg cggaggatga gggaatcgcc ctcgtgcgta
      481 ctctgcagga ggggttcgag cagtacctca ccgcgcggac cgcattcatg gaggccgcgg
      541 ccggccacgc cgagatgaaa agcggcgact gggagcgtca gcgcgccttc ctctcctctc
      601 tggatgcgaa aggaatgcag atccaggcgt atttcctcat cgatcttcag ggagacgggc
      661 actatctcga cggcaaggtg aacaagctgg gcgaccgcga gtacttccag cgcgcccgcg
      721 atacccggaa gaccgtcgtc ggccagcccg tcctctcccg cgcgaccgga gaaacggtgg
      781 tcatcgtcgc ctcccccgtc ttcagcgaga agggagagct gctctccgtg ctctgcggcc
      841 ggatcacggc gaacacgctg atcaccctcg cttcaaccca gaagtggggc gagagcgggc
      901 atgccctcgt caccgacgcg aacggactct tcattgtgca ccccgacaag gagttcatcg
      961 gcgccgtcaa cgcgacgcag gccggggaga agatacccga ggcgttggcc gccgccgcga
     1021 agcgcgctct cggaggggaa caggggattg cgcgctacgt cgagggagga gcggagcagg
     1081 ttgcggccta ttcgcgcgtt ccctcgaccg gatggagcgt ggccatggcc gcatccgtcg
     1141 acgagttcct cgcgcccgtc agggccatgc ggaacgtcat tctgacgtcg ttgctcgtgg
     1201 cggcgctgct ggtcatcgcc gtctcgttct ggatcgccgc aagcgtctcc aggccccttg
     1261 cgcgcgtggt ggcgaagatg gacgaactgg cgacgggaga cttcagctct tccttcgagc
     1321 cccgttccag cctcgtggag atgcggcgcc tttcctcttc gttcaacggg atgatcgctt
     1381 cggtcgcgga cttcgcggga ggcgtcttcg gcgtctcgcg gaagcttctt gcgcgcgcgg
     1441 aggatatgag cgcggcctcg gagcagagca gcgcctcggt gcacgaggtt atcggtctcg
     1501 tcggcaaggt ggcgcagaac tcgcatgacg cggcaagcgc aatcgaggag gcgaacgcgg
     1561 gcgtcggcga agtggcccac ggagcgcagg cgggcgccaa ggccgccgtc gagacgggag
     1621 aacgtgcgca ggagatttcc accgccgcgg aaacaggcgg cagcgcactg gaggagatgg
     1681 tgcacctgat aggggaggtc tcccgttcgg gagcgagcgt caacgccgcg gtgaacaatc
     1741 tcgcctcgtc ggtgtcgggc attacgggct ttgtcaacac gattacgcag atagcggacc
     1801 agacgaacct gctggcgctg aacgcggcga tcgaggccgc gcgcgccgga gaggccggac
     1861 gaggcttcgc tgtcgtcgcc gaggaggtca ggaagctggc ggaggagtcg aaccgcgcag
     1921 cctccgaggt cggccgcgtg atcggcgaga tctcccagaa gaccgaacac gcgctcgccg
     1981 atcagaaagg gagcgtggag cagatacggc agctcgtcgt ccgggcgaag gagacgaagg
     2041 ccgtcatcga cgacgtggtg ctgaaggtcg gcgccattac cgagaacgtg cagtccatcg
     2101 ccgcgacgat gcaggagcag tcggcgagcg cggaggagat gaccgcggga atggacc
//
LOCUS       DAOTIL010000462         2155 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminobacteriaceae bacterium isolate AMR_MDS_3026
            NODE_60574_length_2155_cov_5.182381, whole genome shotgun sequence.
ACCESSION   DAOTIL010000462 DAOTIL010000000
VERSION     DAOTIL010000462.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868050
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminobacteriaceae bacterium (wastewater metagenome)
  ORGANISM  Aminobacteriaceae bacterium
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae.
REFERENCE   1  (bases 1 to 2155)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2155)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:19:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,224
            CDSs (total)                      :: 3,198
            Genes (coding)                    :: 3,184
            CDSs (with protein)               :: 3,184
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2155
                     /organism="Aminobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_60574_length_2155_cov_5.182381"
                     /isolate="AMR_MDS_3026"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:3034609"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..323)
                     /locus_tag="P5201_09525"
     CDS             complement(<1..323)
                     /locus_tag="P5201_09525"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine/ornithine racemase family PLP-dependent
                     enzyme"
                     /protein_id="HRV98820.1"
                     /translation="MTVRYPRLLVSRNRIFENAARVSERCARAGIALTGVTKGVCARE
                     ALVRAMAEGGCDELGDSRIDNLAAIRTMNVGLPLLLLRIPMPSEIAATVRIADRSLVS
                     MAQTIS"
     gene            complement(326..1168)
                     /locus_tag="P5201_09530"
     CDS             complement(326..1168)
                     /locus_tag="P5201_09530"
                     /EC_number="3.1.21.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013048134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0016888 - endodeoxyribonuclease activity,
                     producing 5'-phosphomonoesters [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribonuclease IV"
                     /protein_id="HRV98821.1"
                     /translation="MAIIGAHVSIAGGLEKSIERGEELGCEAIQIFTKNQLQWRSAPL
                     SQSRGERFYRSWAASSIEEIVVHASYLINLAATDATGAKSIAALEDEIERCDLLGIDN
                     LVLHPGSSRGRDVESALDLVAERIASILDRTAGKRVRILLETMAGQGDLLGRNFKQFR
                     RILDALDWPSRVGICMDTCHMFAAGFDMRTEAAYQRLLDQVDDTVGLDRVGCWHFNDS
                     IHDMGRGLDRHAHIGEGTLGYAPFSFVLNDPLWEHTPCILETPQNASGHAGNMSILRK
                     LRGG"
     gene            complement(1185..1766)
                     /locus_tag="P5201_09535"
     CDS             complement(1185..1766)
                     /locus_tag="P5201_09535"
                     /EC_number="3.6.1.55"
                     /inference="COORDINATES: protein motif:HMM:NF012514.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005777 - peroxisome [Evidence IEA];
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003986 - acetyl-CoA hydrolase activity
                     [Evidence IEA];
                     GO_function: GO:0008413 - 8-oxo-7,8-dihydroguanosine
                     triphosphate pyrophosphatase activity [Evidence IEA];
                     GO_function: GO:0010945 - CoA pyrophosphatase activity
                     [Evidence IEA];
                     GO_function: GO:0030145 - manganese ion binding [Evidence
                     IEA];
                     GO_function: GO:0035539 - 8-oxo-7,8-dihydrodeoxyguanosine
                     triphosphate pyrophosphatase activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0009132 - nucleoside diphosphate metabolic
                     process [Evidence IEA];
                     GO_process: GO:0015938 - coenzyme A catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA pyrophosphatase"
                     /protein_id="HRV98822.1"
                     /translation="MNHPVGVSFPSWEELPSGDMRQSAVLVPLFPSGDELRILFLRRS
                     PHLSRHGGEICFPGGMKDADDLSPLHTALRETEEETGISREAVEPLLLLPPEYTVVSN
                     VEVFPVLGIVSGVDPERGLALSPSEIAASYSVDIALFPAEPRRKEILFPSFTKGMRES
                     REHTVSYPEYDLPDGSVVWGVTARILEKVCRLL"
     gene            complement(1823..>2155)
                     /locus_tag="P5201_09540"
     CDS             complement(1823..>2155)
                     /locus_tag="P5201_09540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013048132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016772 - transferase activity,
                     transferring phosphorus-containing groups [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PEP-utilizing enzyme"
                     /protein_id="HRV98823.1"
                     /translation="LKGLSLLKREARGAVCVARSAAEAREKMTKGAVLVARNTDKEYL
                     PAMRLASAVVVEEGGLTSHTAIVALELGIPCIVSAEGALSLLSDGMLVTVDGYRGILY
                     HGRVKLTA"
BASE COUNT          433 a          684 c          624 g          414 t
ORIGIN      
        1 gagatcgtct gcgccatcga gacaagactc ctgtccgcga tccggacagt tgcggcgatt
       61 tcgctgggca tgggaatccg gagaaggagc agcggaagtc cgacgttcat tgtacggatg
      121 gcggccagat tgtcaatcct gctgtcgccg agttcgtcgc agccgccctc cgccatcgcc
      181 cgcacgagag cctcgcgcgc gcatacgccc tttgtcacgc cggtgagggc gatccccgcg
      241 cgagcgcatc gttcagagac cctcgctgcg ttttcaaaga tccgattcct ggagacgaga
      301 agccgcggat agcggactgt cattcttatc ctcctctcag tttacgcaga atagacatat
      361 ttccggcatg gccggaggcg ttctggggcg tttcgagaat acagggcgtg tgttcccaga
      421 gaggatcgtt gaggacgaac gaaaacggag cgtatccgag agtgccttct ccgatgtgcg
      481 cgtgcctgtc gagccctctc cccatatcat ggatgctgtc gttaaaatgc cagcacccga
      541 ccctgtcgag ccccacggta tcgtccacct gatcgagcag gcgctggtac gccgcttcgg
      601 tgcgcatgtc gaatccggcc gcgaacatgt ggcaggtatc catgcaaata cccacgcgcg
      661 acggccagtc cagcgcgtcg agaattctcc tgaactgctt gaaattcctg ccgaggagat
      721 ctccctgacc tgccatggtc tcaagaagaa tacgaacgcg tttccccgcc gtcctgtcaa
      781 gaatagaagc gattcgctcc gccaccaggt caagggctga ttctacgtct cttccgcgcg
      841 acgacccggg gtgaaggacg aggttgtcga tccccagcag gtcgcagcgc tctatttcat
      901 cctccagtgc ggcgatgctc ttcgctccgg tggcgtccgt cgccgccagg ttgatgaggt
      961 acgaagcgtg gacgacgatc tcttcgatag aactcgcggc ccaggagcgg taaaacctct
     1021 cgcccctgga ttgcgagaga ggggcgctcc gccattgaag ctggttcttc gtgaaaatct
     1081 gtatagcttc acatcccagc tcctcgccgc gctcgatgct cttttcaagc cctccggcga
     1141 tggagacatg ggcgcctatt atcgccattg atcttctccc ttcttcacag caggcgacac
     1201 accttttcca atatcctcgc ggtaaccccc cagacgaccg agccgtcggg aagatcgtac
     1261 tccggatagg aaaccgtgtg ttctctgctt tctctcatcc ccttcgtgaa cgaaggaaaa
     1321 agaatttctt ttctccgggg ctccgccgga aaaagggcga tgtccaccga atacgaggcg
     1381 gcaatctccg agggagagag cgcaagcccc cgctccggat cgacgccgga gacaatgccg
     1441 agcaccggaa agacttccac attgctgacc acggtgtact cggggggcag cagaagaagc
     1501 ggctcgaccg cctcccggga aatgccggtc tcctcctcgg tttcgcggag cgcagtgtgc
     1561 aacggcgata ggtcgtccgc gtccttcatc ccgccgggaa agcagatctc tcccccgtgg
     1621 cgtgaaagat ggggcgagcg acgcaaaaaa agaatgcgaa gctcatcccc ggaagggaag
     1681 agcggaacga ggacggcgct ctgccgcatg tccccggacg gaagctcctc ccacgaaggg
     1741 aacgaaacgc cgacggggtg attcatcaac aagcctttcc cggaacgttc cggggagaag
     1801 catcgctcgc cgccccgctc cttcatgccg tcagcttcac ccggccgtgg tagagaatac
     1861 ccctgtaccc gtcgacagtg acgagcattc cgtcggagag aagggagagg gctccctccg
     1921 cgctgacgat gcacggaatt ccaagctcca gcgccacgat cgccgtgtgg gaggtaagcc
     1981 ccccctcttc gacgacaaca gcggaggcga gccgcatggc cggaagatac tctttgtcgg
     2041 tattcctggc gacgagcacg gcgcctttgg tcatcttctc cctggcctcg gcggcgctcc
     2101 gagccacaca gacggcgccc ctcgcctctc tcttgagcag cgagagtcct ttcag
//
LOCUS       DAOTIL010000463         2154 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminobacteriaceae bacterium isolate AMR_MDS_3026
            NODE_60619_length_2154_cov_3.124345, whole genome shotgun sequence.
ACCESSION   DAOTIL010000463 DAOTIL010000000
VERSION     DAOTIL010000463.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868050
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminobacteriaceae bacterium (wastewater metagenome)
  ORGANISM  Aminobacteriaceae bacterium
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae.
REFERENCE   1  (bases 1 to 2154)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2154)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:19:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,224
            CDSs (total)                      :: 3,198
            Genes (coding)                    :: 3,184
            CDSs (with protein)               :: 3,184
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2154
                     /organism="Aminobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_60619_length_2154_cov_3.124345"
                     /isolate="AMR_MDS_3026"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:3034609"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(298..1284)
                     /locus_tag="P5201_09545"
     CDS             complement(298..1284)
                     /locus_tag="P5201_09545"
                     /inference="COORDINATES: protein motif:HMM:NF024824.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HRV98824.1"
                     /translation="MNKKKLLHIFLLFLLFFSSHISFTRLQSAHANGVIVTAPENAQQ
                     KAAPKKKPSVKKQVQPTPPPIQEEKEVIVLPTPLDQALVLMEKRFFTRATALLEQIVE
                     REPLNAHAWYALGRAYEARGFFPKAQKALRRSLEIDPSFNELSRFLEYPAEGDRQPLW
                     DPKRPARIEEIPVAIDGFTIMPPSETLVPPVSPVQPPIQYASPTAPVQRQAEYAPPAP
                     QAPQQQVQYTPPAPPIHGDQQLLHNSISPTSGVPVRVVQPGTPPNLSPGLLTESPSPM
                     LPSSPLSPESEAPLYIPPAPYSPESIIASPDILTEGGKPVYTPPPPGAELPQ"
     gene            complement(1281..>2154)
                     /locus_tag="P5201_09550"
     CDS             complement(1281..>2154)
                     /locus_tag="P5201_09550"
                     /inference="COORDINATES: protein motif:HMM:NF027852.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="FlgT C-terminal domain-containing protein"
                     /protein_id="HRV98825.1"
                     /translation="EIWESKYYPVNVLEQKMTEYLASLLRQNPFTDVSILDSSQAQRW
                     MDDPYSRQGAFAVQMELYSVLAKEREVLGSFEKGDVSLRVRIYSALDAELETSYISSG
                     KDQRFTFNPGDDRLYLLNTRTSIIDIFYRNGLDFLRLAPADKGQKMSRLTWQQFSGTP
                     YWQAFKNAIKGVATGIADMGDGELAVIGRIIAPTANSTRRNREYIITLGRQDALAVGD
                     ILQVVRGDTYVTVDPENPVVVLQRVVGNVKVIKTMNEQAIVRLLNEKSQDPVQLNDLV
                     TAPPYGTKKVAPLR"
BASE COUNT          522 a          497 c          571 g          564 t
ORIGIN      
        1 atggccggta gacataaaag gacattcctg aatacacggg ttgcgccacg agaaccggtt
       61 cgacaacgta tgttgtcgct gttgtagatg atggtaggag agagagcagg actgttaaac
      121 tgagtacgat gagtattctt cttctcatgg taaaccatcc tcctttggga tttttctcag
      181 gagcaaaaac acggaaaacg ctttttctga gtcgttatgt tctagagatg caaagtgttc
      241 tcttggtaca ttttcccgtc cgacaatatt atagaatgaa atgaaacaga ttgctagtta
      301 ctgaggaagt tccgctcccg ggggcggagg agtatatacc ggtttgcctc cttctgtgag
      361 aatatcaggc gatgcgataa tactctcagg gctatacgga gccgggggaa tatacagtgg
      421 cgcctcactc tccggtgata atggagagga ggggagcatt ggtgaaggac tctccgtcaa
      481 taagccggga gagagattgg gaggcgtgcc tggctgcact acacgaactg gaacgccgga
      541 ggttggtgaa atggaattgt gcaacagctg ctggtcaccg tgtatcggcg gagccggggg
      601 ggtgtactgt acttgttgct gaggagcctg aggagcagga ggggcgtatt ctgcttgccg
      661 ctgtaccggt gcagtaggag acgcgtattg aataggcggt tgtactggac tcaccggagg
      721 aacaagagtc tccgaaggag gcatgattgt aaaaccgtca atagcaacag gaatctcctc
      781 tattctcgcc ggacgtttgg gatcccacaa gggctgtctg tccccttctg caggatactc
      841 caaaaaacga gacaactcgt taaaagaagg gtctatttcc agtgatctgc gaagagcttt
      901 ctgggctttt ggaaaaaacc cccgcgcctc atatgctctt ccgagtgcgt accaagcatg
      961 tgcgttcaac ggttcgcgtt ctacaatctg ttccaatagg gcagttgctc tcgtaaagaa
     1021 tctcttttcc ataagaacca gcgcctgatc taaaggcgtg ggaagcacta ttacctcttt
     1081 ttcctcctgg attggcggag gcgttggttg cacctgtttt ttcacggagg gtttcttctt
     1141 gggcgcggct ttttgttgtg cattctcagg cgcagtcaca atcactccgt ttgcatgcgc
     1201 tgactgcaaa cgcgtgaaag aaatatggga cgaaaaaaac aacagaaaga gcagaaatat
     1261 gtggagtagt ttttttttgt tcatcgcaaa ggcgccacct tttttgtccc gtatggagga
     1321 gctgttacaa gatcgttcaa ctgaacgggg tcttgtgatt tttcattgag caggcgaacg
     1381 atagcctgtt cgttcatggt tttgatcacc ttgacgttgc ctacaactct ctgtaaaacc
     1441 acaacggggt tttcagggtc gaccgttacg tatgtatcgc ctctgactac ctgaagtatg
     1501 tcgccgacag cgagagcgtc ctgtcgaccg agagttatta tgtactctct gttgcgtcgt
     1561 gtcgagtttg ccgtaggggc aataatgcgc cctataacag cgagttcgcc gtcccccata
     1621 tcggctattc ctgtagccac tcccttaatg gcgttcttaa aagcttgcca ataaggggtg
     1681 ccggaaaact gttgccatgt aagacgactc attttctgcc ccttatctgc cggggcaagt
     1741 cgaaggaaat caaggccgtt tctatagaaa atatcgataa tgctcgttcg tgtgttcagc
     1801 agatacaacc tgtcgtcgcc tggattgaac gtgaatcgct ggtctttccc cgaggaaata
     1861 taggaagttt caagctccgc atcaagagcg ctgtaaattc tgactcgcaa ggacacatca
     1921 cctttttcaa aagagccgag aacctcgcgc tcctttgcga gtaccgagta gagttccatt
     1981 tgcactgcga aagcgccttg cctggagtaa ggatcatcca tccagcgttg cgcctgcgag
     2041 ctatccagta tcgaaacatc cgtaaagggg ttttgccgca ggagagaggc cagatactca
     2101 gtcatcttct gttcaagcac attcactgga tagtacttac tctcccaaat ttcg
//
LOCUS       DAOTIL010000464         2152 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminobacteriaceae bacterium isolate AMR_MDS_3026
            NODE_60689_length_2152_cov_4.198379, whole genome shotgun sequence.
ACCESSION   DAOTIL010000464 DAOTIL010000000
VERSION     DAOTIL010000464.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868050
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminobacteriaceae bacterium (wastewater metagenome)
  ORGANISM  Aminobacteriaceae bacterium
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae.
REFERENCE   1  (bases 1 to 2152)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2152)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:19:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,224
            CDSs (total)                      :: 3,198
            Genes (coding)                    :: 3,184
            CDSs (with protein)               :: 3,184
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2152
                     /organism="Aminobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_60689_length_2152_cov_4.198379"
                     /isolate="AMR_MDS_3026"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:3034609"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1715
                     /locus_tag="P5201_09555"
     CDS             <1..1715
                     /locus_tag="P5201_09555"
                     /inference="COORDINATES: protein motif:HMM:NF023478.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF3536 domain-containing protein"
                     /protein_id="HRV98826.1"
                     /translation="ESATLGRLVSVATDGETFGHHHKYGDMALAYCLDIIERSRSASL
                     TIFGEYLDSTPPVDEVRIVENSSWSCVHGVGRWKDDCGCSAHSRPGWNQRWRGPLREA
                     LDTLRDAAAPLFEKEAGMLFPDPWGARNRYIEVFFDHTRSEATSFLQREAGRALSREE
                     QVRALRLMELQRNLMLMYTSCGWFFDEISGIETLQVMAYAARALELCWRLFPREDFES
                     PFKNLLSKAPSNIVEFVNGKRVYDIFVTQMRSDLLRAGAHYAVSALFSGDEGDVRANL
                     GDVFYAYRVLSCSLERKDEGARRYALGYLRIRSEITRDEKELFVAALYRGGRNVLCGV
                     AEKPEPEDGNGIREKIEEALRSEDEEGLVSFFGHNTYSLRHLFKDEQRRILNLIIAED
                     VEAVVSMLRKTISDSSELLSFLSELAMPIPEAFRSAAEVTLNDELKRAFEADALDLDF
                     VSQRIDEGERWGAGVDVLFLRHAAMRRLERLFLLLEREPFEENTLRSIIELLSFLKKR
                     GWEINLWDEQNTFAGIIASNEDLSSCGLLYETLGKMLLVRHSRVESDFADRVTPRSRQ
                     KGVM"
     gene            1880..>2152
                     /locus_tag="P5201_09560"
     CDS             1880..>2152
                     /locus_tag="P5201_09560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013048722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter substrate-binding protein DctP"
                     /protein_id="HRV98827.1"
                     /translation="MKFSKVSALFVLFALFAVMVPGYAEAAVEVKMSYNGPGDVENNA
                     VHLYAVNFKKFVEEGTGGEVTIALFPDSQLGNEEERMELLSKQGMNQ"
BASE COUNT          441 a          558 c          646 g          507 t
ORIGIN      
        1 ccgaaagtgc gacgctgggg cgccttgtct cggttgcaac ggacggcgag acgttcggac
       61 accatcataa gtacggcgac atggcgctgg cctactgcct cgacataatc gaacgatcca
      121 ggtccgcatc gctcacgatc ttcggcgaat acctcgactc gacgcccccc gtcgacgagg
      181 tgcggatcgt cgagaactcc tcctggagct gcgttcacgg cgttggaagg tggaaggatg
      241 attgcggctg ttccgcgcac tcccgccccg gatggaatca acgctggaga gggcctctca
      301 gggaggcgtt ggatacgctg cgggacgctg ccgctcctct ttttgaaaag gaagcgggga
      361 tgctttttcc cgatccgtgg ggcgcgcgca accggtatat agaggtcttc ttcgaccata
      421 cccgttccga agccacatcg tttctgcaac gggaggccgg aagagctctc tcgcgcgagg
      481 aacaagtcag agcccttcgt ctgatggagc ttcagcgaaa tctgatgttg atgtacacca
      541 gctgcggctg gttcttcgac gagatctccg gtatcgaaac actccaggtc atggcctacg
      601 ctgcccgcgc gcttgaactg tgctggcgtc tcttcccccg ggaggatttt gaatcgcctt
      661 tcaagaacct tctatcaaaa gctcccagca acatcgttga attcgtcaac ggaaaacgcg
      721 tctacgatat ttttgtcacg cagatgcgtt cggatctgct ccgcgccgga gcgcattacg
      781 ccgtctccgc cctcttttca ggcgacgagg gggatgtgcg tgcgaatctt ggagacgttt
      841 tctatgctta cagggttttg tcgtgttccc ttgaacgaaa agatgaagga gcccgccggt
      901 atgctctcgg ctatcttcga atccgttccg aaattacacg ggatgaaaaa gagctttttg
      961 tcgccgctct ctaccgcgga ggacggaacg tgctgtgcgg cgtggccgag aagccggagc
     1021 cggaagacgg aaacggtatc cgtgagaaga tcgaggaggc tcttcgaagc gaggatgaag
     1081 aggggctggt ttccttcttc gggcacaaca cctattcact gcgccatctc ttcaaagacg
     1141 agcaaaggcg tatcctaaat ttgatcatcg ccgaggatgt cgaggccgtc gtttcaatgc
     1201 tcaggaagac gatttcggac tcatcggaac tcctctcctt tctctccgag ctcgcgatgc
     1261 cgattccaga ggcgttccgg agcgctgccg aagtgacgct taatgacgag ctgaagcgcg
     1321 ccttcgaggc ggatgctctt gatcttgatt ttgtgtcgca gcggatagac gaaggagagc
     1381 gctggggcgc cggagtcgat gttctttttc tgcgtcacgc cgcgatgcgg cgtctcgaac
     1441 gcctctttct cctgctggaa cgggagccgt ttgaagaaaa caccttgcgg agcataatag
     1501 agcttctttc tttcttaaaa aaaagaggtt gggagatcaa tttgtgggat gagcagaaca
     1561 cattcgccgg aatcatcgcg tcgaacgagg atctttcatc ctgcggcctg ctctacgaga
     1621 cattgggaaa gatgctgttg gttcggcact cccgtgtgga aagcgatttt gcggataggg
     1681 taactccccg ttcgcggcag aaaggggtga tgtgacgcgc ttttctcttg atgagacgaa
     1741 agccctgcat cgaatcgctt ttttgtggtg taatagtatc cgggtattta atggttcgga
     1801 gagatggtct ctccgttttc cgctctccgg gtgtcctgcc ggaggggtgt tctgtatttt
     1861 tcaagggagg gtatgtgcaa tgaagttcag taaggtatcg gctcttttcg ttctgttcgc
     1921 gctgtttgcc gtcatggttc cggggtacgc cgaggcggct gtcgaggtca aaatgtccta
     1981 caacggcccc ggcgatgtgg agaataacgc ggtgcatctg tacgcggtga acttcaagaa
     2041 attcgtggaa gagggcaccg gcggtgaagt taccatcgcg ctcttcccgg acagccagct
     2101 gggcaacgag gaggagcgga tggaacttct ctcgaagcag gggatgaacc ag
//
LOCUS       DAOTIL010000465         2149 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminobacteriaceae bacterium isolate AMR_MDS_3026
            NODE_60811_length_2149_cov_3.270774, whole genome shotgun sequence.
ACCESSION   DAOTIL010000465 DAOTIL010000000
VERSION     DAOTIL010000465.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868050
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminobacteriaceae bacterium (wastewater metagenome)
  ORGANISM  Aminobacteriaceae bacterium
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae.
REFERENCE   1  (bases 1 to 2149)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2149)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:19:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,224
            CDSs (total)                      :: 3,198
            Genes (coding)                    :: 3,184
            CDSs (with protein)               :: 3,184
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2149
                     /organism="Aminobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_60811_length_2149_cov_3.270774"
                     /isolate="AMR_MDS_3026"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:3034609"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..623)
                     /locus_tag="P5201_09565"
     CDS             complement(<1..623)
                     /locus_tag="P5201_09565"
                     /inference="COORDINATES: protein motif:HMM:TIGR01891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004180 - carboxypeptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HRV98828.1"
                     /translation="MHDIQRKAQAMQERIVQWRRALHAMPEVGFDTPGTERFIVEELQ
                     KMGVTEIRHGDGKKGIVALVQGKKPGGVLGIRADYDALHMTEETGLPFAATNGNMHAC
                     GHDAHAAILLGTANLLLDCADELAGTVKLIFQPSEENGKGALAMIKDGVLEEPKMDAI
                     IGLHIGSLWKGLNSGEVGYRYGALMAAADWFKITFGGKGGHGATPHLT"
     gene            902..>2149
                     /gene="lsrK"
                     /locus_tag="P5201_09570"
     CDS             902..>2149
                     /gene="lsrK"
                     /locus_tag="P5201_09570"
                     /EC_number="2.7.1.189"
                     /inference="COORDINATES: protein motif:HMM:NF008187.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0071518 - autoinducer-2 kinase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0009372 - quorum sensing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="autoinducer-2 kinase"
                     /protein_id="HRV98829.1"
                     /translation="MASGKYLMAIDAGTGSARAVLFDESGRQVAVSQAEWSHKEDPRF
                     PGSMDFDVEGNWKLIVGCVKGVLEKSGADPRSVAAIATTSMREAIVLYDGAGKEIWAC
                     ANVDARSSAEVVKLMRLRPGIEEELYAVSGQTFSLNALPRLLWVKENLPDVYESVRAV
                     NMLNDWIAYKLSGVLTAEPSNGCTSGIFDIAARKWKPEIAASCGLRTDIYPAVTESAE
                     IIGRVSADCAALTGLSTETLVVSGGGDAQLGCVGVGAVADGQAAVLGGSFWQYEFNTA
                     VPVMDPKCRVRVNCHAVPGVFQYEAIAFFPGLVMRWFRDAFCFEEKRVEDEGGPDAYM
                     QLAEGASKIPAGSHGMMCAFSDVMNYIAWKHAAPTFSNFSLDAEKFNKYTFFRSLMEN
                     AALVTRGHLQLVEEVTGRRPEGIV"
BASE COUNT          360 a          658 c          700 g          431 t
ORIGIN      
        1 gtgagatgcg gcgtggcgcc gtgaccgccc ttccctccga aggtaatctt gaaccagtct
       61 gccgcggcca tcagcgcgcc gtaccggtac ccgacctcgc cggagttcaa gcccttccac
      121 aggcttccta tgtgcagccc gatgatggcg tccattttcg gctcctcgag gacgccgtcc
      181 ttgatcatcg cgagcgcgcc cttgccgttc tcctcggagg gctggaagat cagtttcacc
      241 gtccccgcca gctcgtcggc gcagtccagc agcaggttcg ccgtccccag caggatcgcc
      301 gcgtgcgcgt cgtggccgca ggcgtgcata ttgccgttgg tcgccgcgaa ggggaggccg
      361 gtctcctcgg tcatgtgcag cgcgtcgtaa tcggcgcgga tgccgagtac gcctcccggc
      421 ttctttccct gtacgagggc cacgatgccc ttttttccgt ctccgtggcg gatctccgtg
      481 acgcccatct tttgaagctc ctcgacgatg aatcgctccg tgccgggggt gtcgaagccg
      541 acctccggca tggcgtgaag ggcccgccgc cactggacga tccgttcctg catggcctgc
      601 gctttgcgct gtatgtcgtg catgagatca tctccctgta tcgtgtttgt tcatgttcct
      661 attctgtagt cgtaatgctc gtgtgagcag ataataccat ggagcggcgg ggattccaag
      721 atgttccgcg ggcgtccggg gttgcttcgg gaagccgtcg ctttctgcaa gaaaaccgaa
      781 tttgcattga caatactgta ccatttttcc tataatggca tccgtagaga ctttctcaca
      841 tatatgagat gatttcacat atgtgattag agacgccgat tctttccagg gggtgagcgc
      901 tttggcttcg ggtaagtatc tgatggcgat cgacgcggga acgggcagcg cgcgggccgt
      961 gctcttcgac gagtccggac ggcaggttgc ggtgtcgcag gcggagtgga gccacaagga
     1021 ggatccccgg tttcccggct cgatggattt cgacgtggag gggaactgga agctgatcgt
     1081 cgggtgcgtc aagggcgtgc ttgagaagag cggcgccgat ccccggtcgg tcgcggcgat
     1141 agccaccacc agcatgcgcg aggcgatcgt cctctacgac ggggcgggca aggagatatg
     1201 ggcctgcgcc aacgtggacg cccggtcctc ggcggaggtc gtgaagctga tgcgccttcg
     1261 tcccgggatc gaagaagaac tctacgctgt ctccggccag acgttctcgc tgaacgcgct
     1321 ccccaggctg ctctgggtga aggagaacct tccggacgtt tacgagtcgg tccgcgccgt
     1381 gaacatgctc aacgactgga tcgcctacaa actctcgggc gtgctgacgg cggagccgtc
     1441 caacggctgc accagcggta tcttcgacat cgccgccagg aagtggaagc cggagatcgc
     1501 ggcgtcgtgc gggttgcgca cagacatcta ccccgcggtg accgagagcg ccgagataat
     1561 cggcagggtg agcgcggact gcgccgccct cacgggcttg agcacggaga cgctcgtcgt
     1621 ctcgggcggc ggcgacgcgc agctcgggtg cgtgggcgtg ggcgcggtgg cggacggaca
     1681 ggccgccgtg ctcggcggga gcttctggca gtacgaattc aacaccgccg tccctgtgat
     1741 ggacccgaaa tgccgcgtgc gggtgaactg ccacgccgtg ccgggcgtct tccagtacga
     1801 ggccatcgcc ttcttccccg gcctggtcat gcgctggttc cgcgacgcct tctgcttcga
     1861 ggagaagcgc gtggaggacg aaggaggtcc ggacgcctac atgcagctcg ccgagggcgc
     1921 ctcgaagatc cccgcaggat cgcacgggat gatgtgcgcc ttctccgacg tcatgaacta
     1981 tatcgcatgg aagcacgcgg ctcccacctt ctccaacttc tcgctcgacg ccgaaaagtt
     2041 caataagtac accttcttcc gctccctgat ggagaacgcg gcgcttgtga cgcgcggcca
     2101 tctgcagctt gttgaggagg tcacgggaag gcgcccggaa ggaatcgtc
//
LOCUS       DAOTIL010000466         2146 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminobacteriaceae bacterium isolate AMR_MDS_3026
            NODE_60930_length_2146_cov_3.250120, whole genome shotgun sequence.
ACCESSION   DAOTIL010000466 DAOTIL010000000
VERSION     DAOTIL010000466.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868050
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminobacteriaceae bacterium (wastewater metagenome)
  ORGANISM  Aminobacteriaceae bacterium
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae.
REFERENCE   1  (bases 1 to 2146)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2146)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:19:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,224
            CDSs (total)                      :: 3,198
            Genes (coding)                    :: 3,184
            CDSs (with protein)               :: 3,184
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2146
                     /organism="Aminobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_60930_length_2146_cov_3.250120"
                     /isolate="AMR_MDS_3026"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:3034609"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..316)
                     /locus_tag="P5201_09575"
     CDS             complement(<1..316)
                     /locus_tag="P5201_09575"
                     /inference="COORDINATES: protein motif:HMM:NF012512.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal-phosphate dependent enzyme"
                     /protein_id="HRV98830.1"
                     /translation="MNLARFPRRRYTEGRTPLEFLERLSAKLGGPEIWIKRDDMLGFL
                     PGGNKTRKLEFLMADALAQGADAVITCGAVQSNHCRLTLAAARKEGLECHLVIEERVP
                     GSY"
     gene            complement(344..1282)
                     /locus_tag="P5201_09580"
     CDS             complement(344..1282)
                     /locus_tag="P5201_09580"
                     /inference="COORDINATES: protein motif:HMM:NF015558.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative sulfate exporter family transporter"
                     /protein_id="HRV98831.1"
                     /translation="METLRTHALFFLCLALAVALALLPPTAPYAVGMALVLGAAVSNL
                     APGSAPPGLGKMRKLALNSAVVLFGFGLNIRQVISVGGQGFWQTAVSLAVIISLGFFL
                     LRFFKVEAATLKLVTFGTSICGGSAIAAVSSVMKVRDEDIGVAMGVVFLLNAVALVAF
                     PLLASLLPLTPEQYGVWCALSIHDTSSVVGAAAVLGDASLQTATIMKLTRTLWITPIV
                     FLISLRQGEKGKLSIPLFIVFFLLASVTASVLPFAPFFKTLASSGKVLMAVALYIVGF
                     GLHRSVLKKVGAQGMLFGMLLWAVSIVMGYLLASMS"
     gene            complement(1401..>2146)
                     /locus_tag="P5201_09585"
     CDS             complement(1401..>2146)
                     /locus_tag="P5201_09585"
                     /inference="COORDINATES: protein motif:HMM:NF012245.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HRV98832.1"
                     /translation="EYLVYVPGRTIDIKKKPGDKLTEGSAGHRCMMEKRKIVKEFKKE
                     DSPFGFPYVAASVPIFDASGKAVGCVITAENITRQETMRETAELLRASTGQIADSLQT
                     MTGQMEEMRAAGEMLNSITSQAVSKVEGTEQVFGVIEGVAKQTNMLGLNASIEASRLG
                     DQGKGFGVVAEEVRKLAVRSSDSARQIKEILEYFKNSTIEINSSTDTLLHIIKEQSKI
                     IETIAASSEEMASVSAMLEKLSSSLGQGS"
BASE COUNT          418 a          687 c          654 g          387 t
ORIGIN      
        1 agtagcttcc cggaacgcgc tcctctatga cgaggtgaca ctcgagccct tccttcctcg
       61 cggcggcgag ggtgaggcgg cagtgattcg actgcaccgc gccgcaggtg atcacggcgt
      121 ctgcgccctg cgccaacgcg tcggccataa ggaattcgag cttccgagtc ttgttccctc
      181 ccgggagaaa gccgagcatg tcgtcccgct tgatccatat ctccgggccg ccgagctttg
      241 ctgaaagccg ttcgagaaat tccaaagggg tccgcccctc ggtatagcgt cttctgggaa
      301 agcgtgcgag attcatctta ttccatctcc tttgtgtcga atatcagctc attgatgcga
      361 ggaggtaccc catgacgatg gagaccgccc agagcagcat gccgaagagc atcccctgcg
      421 cgccgacctt cttgaggacg ctgcggtgca ggccgaagcc gacgatatag agcgcaaccg
      481 ccatcagtac cttcccggac gaagcgagcg tcttgaagaa cggggcgaag ggaaggacgg
      541 acgccgtcac cgaggccagc agaaagaaga cgatgaagag cgggatggag agcttgccct
      601 tctcgccctg gcgcagcgag atcaggaaga cgatgggcgt gatccagagt gtccgggtga
      661 gcttcatgat ggtcgcggtc tgcagcgacg catcccccag caccgccgcc gcccccacaa
      721 ccgagctggt gtcgtggatg ctcaacgcgc accagacgcc gtactgctcc ggcgtgagcg
      781 gaagcaacga cgcaagcagc ggaaaggcga cgagcgccac ggcgttcagc agaaagacga
      841 cgcccatggc gacgccgatg tcctcgtcgc gaaccttcat caccgaggac acggcggcaa
      901 tggcgcttcc gccgcagatg gacgtgccga aggtcaccag cttcagcgtc gccgcctcga
      961 ccttgaaaaa ccggagcaga aaaaaaccga gcgagatgat caccgcaagg ctcaccgcgg
     1021 tctgccagaa cccctggccg ccgacggaga tcacctgtcg gatgttcaga ccgaacccga
     1081 agaggacgac ggcgctgttc agcgcaagtt tgcgcatctt ccccagtccg ggaggcgccg
     1141 agccgggggc gaggttgctc accgccgcgc ccagtacgag cgccataccg acggcgtagg
     1201 gcgcggtcgg aggaagcagc gccagcgcca ctgcaagggc gaggcagaga aaaaagagcg
     1261 cgtgggtgcg aagagtttcc actacatatt cctccttggc agaaaacgca ggactcggga
     1321 gaaggcaacg tctcgcgatc ctgcgatgtt tttcaaaaag agcgggctga cggagaccgt
     1381 ccttcccgcc gggacacgtc tcagctccct tgcccgagcg aggaggagag cttctccagc
     1441 atcgccgaga cggaggccat ctcttcgctg gacgcggcga tggtctcgat gattttcgac
     1501 tgttccttga tgatgtggag cagggtgtcg gtgctgctgt tgatctcgat cgtggagttc
     1561 ttgaagtact ccaggatctc cttgatctgc cgtgcggagt cggacgagcg gacggcgagc
     1621 ttccgcacct cctccgccac gacgccgaac cccttcccct gatctcccag gcgggaggct
     1681 tcgatcgagg cgttcagccc gagcatattc gtctgcttcg ccacgccctc gatgacgccg
     1741 aacacctgtt cggttccctc caccttgctc acggcctgcg atgtgatgga gttcagcatc
     1801 tcgcccgccg cgcgcatctc ctccatctgt ccggtcatcg tctggagaga gtccgctatc
     1861 tgccccgtgg aggcgcgcag cagctccgcg gtttcccgca tcgtctcctg acgggtgatg
     1921 ttctccgccg tgatgacgca gccgaccgcc tttccggaag cgtcgaagat cggcacggag
     1981 gccgcgacgt acgggaagcc gaacggcgaa tcctccttct tgaactcctt gacgatcttc
     2041 cgcttttcca tcatgcaccg gtgccccgcc gacccctcgg tcagcttgtc accgggtttc
     2101 ttcttgatgt cgatcgtcct gcccggcaca tagacgagat actcgt
//
LOCUS       DAOTIL010000467         2143 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminobacteriaceae bacterium isolate AMR_MDS_3026
            NODE_61040_length_2143_cov_3.695402, whole genome shotgun sequence.
ACCESSION   DAOTIL010000467 DAOTIL010000000
VERSION     DAOTIL010000467.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868050
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminobacteriaceae bacterium (wastewater metagenome)
  ORGANISM  Aminobacteriaceae bacterium
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae.
REFERENCE   1  (bases 1 to 2143)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2143)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:19:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,224
            CDSs (total)                      :: 3,198
            Genes (coding)                    :: 3,184
            CDSs (with protein)               :: 3,184
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2143
                     /organism="Aminobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_61040_length_2143_cov_3.695402"
                     /isolate="AMR_MDS_3026"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:3034609"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..499)
                     /locus_tag="P5201_09590"
     CDS             complement(<1..499)
                     /locus_tag="P5201_09590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013048088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH domain-containing protein"
                     /protein_id="HRV98833.1"
                     /translation="MVVDLLELLLSLGTSFGFILIVLFILASAVKIVPEYQRVVVFRV
                     GRLVGAKGPGIVLVIPIIDKVVRVDLRVVTLDVPVQEVITRDNVPIKVNAVVYFRVMD
                     PAFSVVEVENYILATSLLSQTTLRSVVGGADLDEVLSSREKINSELQKIIDERTDSWG
                     IKVSAV"
     gene            complement(503..1864)
                     /locus_tag="P5201_09595"
     CDS             complement(503..1864)
                     /locus_tag="P5201_09595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014806478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nodulation protein NfeD"
                     /protein_id="HRV98834.1"
                     /translation="MKNCLKKSAMFLVLLFALCACPAFSAQAGTVPSVEGKVFSAALD
                     GTVGVSMENFVTAVLTRAEEEKGGLVIFRLDTPGGLVSSMRAITAAILESSVPVVVWV
                     SPQGARAASAGAFILQAAHVAAMAPGTNVGAAHPVMASGQDAPDKEMKRKITNDLAAQ
                     IRSLAQLRKRNADLAERMVTHSISLTAEEALAEGAADLSAPDLESLLLGVRGRRVLVR
                     GEEKELVPEIGTPVEITMTSREKALQFISSPDIAYLLLTAGILMIVLEVMSPGGFVLG
                     TAGAVMVLLGSFGLRMLPFNWAGIVLLVAGIGVLVLDLMVGGIGILSLFGLASLVTGG
                     LILFRAPGGELLNVSMSVIGGMSLALGLFFLGALFLVVRSMNKKAVSGREGMKGKKAV
                     VVEDLLPEGLVRCHGEIWKARSSSGAFFSKGDEVLVTELDGITLIVRGETGKELPGEE
                     KEE"
     gene            complement(1861..>2143)
                     /locus_tag="P5201_09600"
     CDS             complement(1861..>2143)
                     /locus_tag="P5201_09600"
                     /inference="COORDINATES: protein motif:HMM:NF017200.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="magnesium chelatase domain-containing protein"
                     /protein_id="HRV98835.1"
                     /translation="LLLREPAADLALCAALASAVRDVPLPGNCVFLGEVGLAGEIRPV
                     VRLARRLQEAARLGFTQAIVSAREGGGGKAPLDVVRVSTLKDALKAVIS"
BASE COUNT          488 a          739 c          544 g          372 t
ORIGIN      
        1 ccacggcact gaccttgatg ccccaggaat cggtccgctc atcgatgatc ttctgcaact
       61 cgctgttgat tttttccctc gacgagagca cttcgtcaag atcggctccg ccgacgaccg
      121 aacgaagtgt cgtctgcgag agaaggcttg tcgcaagaat atagttctcc acctcgacca
      181 cggaaaacgc agggtccatg acgcggaaat agacgaccgc gttgaccttg atcgggacgt
      241 tgtcccgcgt gatgacctcc tgcaccggca cgtcgagcgt caccacgcgg aggtccactc
      301 gcaccacctt gtcgatgatc gggatcacca gcacgatgcc gggtcccttc gcgccgacaa
      361 ggcgtcccac ccgaaagacg accacgcgct ggtactcggg tacgatcttc accgcggacg
      421 ccaaaatgaa cagaacaatg agaatgaacc cgaaggacgt ccccaaactg agcaacagct
      481 ccaaaagatc gactaccata gtttattcct ccttttcttc gccgggaagc tctttcccgg
      541 tctctccacg aacaatcagc gtaattccgt cgagctctgt gacaagcacc tcgtcaccct
      601 tcgagaaaaa ggcccccgaa gagctccggg ccttccagat ctctccgtga caccggacca
      661 gcccttcagg gagcaggtcc tccacgacca cagccttctt tcctttcatc ccttctctgc
      721 ccgagacagc cttcttattc attgaacgaa caacgaggaa gagcgcgccg aggaagaaga
      781 gaccgagtgc aaggctcatg ccgcctatga cgctcatgga tacgttcaac aactcacccc
      841 ccggagcccg aaagagaatc agcccccctg tcacaagaga agcaaggccg aaaaggctca
      901 gaataccgat tccgcccacc ataagatcga ggaccagcac gccgattcct gcaaccagga
      961 ggacaatacc cgcccagttg aacggcagca tccgaagtcc gaacgacccc agaagcacca
     1021 tcacggcgcc ggccgttccc agcacaaacc ctcccggaga catcacttcg agcacgatca
     1081 tcaaaattcc cgctgtaagc agcagatagg cgatatcggg gcttgagatg aactggagcg
     1141 ccttctcgcg cgacgtcata gtgatttcca ccggcgtccc gatctccgga acaagctcct
     1201 tctcctcgcc cctcacgagc actcttcttc cgcgaacgcc gagaagcagc gactccaaat
     1261 cgggagccga cagatccgcc gccccctcgg ccagcgcttc ctccgctgtg agcgaaatgc
     1321 tatgggtcac catccgctcc gccagatccg cgttcctctt tctgagctgg gcgagggagc
     1381 ggatctgggc ggccagatcg ttggtgatct tccgcttcat ttccttgtcc ggcgcgtcct
     1441 gtcccgacgc catcacagga tgggcggcgc cgacgttcgt tccgggggcc atcgcggcta
     1501 cgtgcgccgc ttgcagaata aaggctccgg ccgaggcggc cctcgctccc tgcggagaga
     1561 cccagaccac gacgggaaca gacgattcga gtattgccgc ggtaatggcc ctcatcgagc
     1621 tgaccaggcc tcccggagta tccaggcgga aaataacgag tccgcctttt tcctcctctg
     1681 cacgcgtaag caccgccgtc acaaaattct ccatcgacac gcccaccgtt ccgtcgagcg
     1741 ccgcggaaaa caccttgcct tccacggacg gaaccgtacc agcctgcgca gaaaacgccg
     1801 gacacgcgca cagtgcaaac agcaaaacca ggaacatggc gctctttttt aaacagtttt
     1861 tcatgatatc accgccttta aagcatcctt gagcgtcgaa acccggacga catcaagagg
     1921 ggcttttccc cctcctcctt cgcgggcgct gacgatcgcc tgtgtaaaac ccagccgcgc
     1981 agcttcctgc aagcgcctgg ccagcctgac caccggacgg atctcccccg cgagcccgac
     2041 ctcccccaga aagacgcagt ttccgggaag cggaacgtcc cgcaccgcgg acgcgagcgc
     2101 cgcgcagagc gccagatccg cagccggctc gcgcaaaaga aga
//
LOCUS       DAOTIL010000468         2141 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminobacteriaceae bacterium isolate AMR_MDS_3026
            NODE_61107_length_2141_cov_3.461170, whole genome shotgun sequence.
ACCESSION   DAOTIL010000468 DAOTIL010000000
VERSION     DAOTIL010000468.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868050
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminobacteriaceae bacterium (wastewater metagenome)
  ORGANISM  Aminobacteriaceae bacterium
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae.
REFERENCE   1  (bases 1 to 2141)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2141)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:19:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,224
            CDSs (total)                      :: 3,198
            Genes (coding)                    :: 3,184
            CDSs (with protein)               :: 3,184
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2141
                     /organism="Aminobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_61107_length_2141_cov_3.461170"
                     /isolate="AMR_MDS_3026"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:3034609"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..780
                     /locus_tag="P5201_09605"
     CDS             <1..780
                     /locus_tag="P5201_09605"
                     /inference="COORDINATES: protein motif:HMM:NF013227.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegT/DnrJ/EryC1/StrS family aminotransferase"
                     /protein_id="HRV98836.1"
                     /translation="KVIAPVSYAGYPVDIAPFLESAGSCGAVVVEDACHALGAERNEV
                     RVGKEAHMTAFSFHPVKHITTGEGGMVATDSEEFARRLRLFRTHGIEKEPRFMTAPPD
                     GPWATEMQILGFNYRLSELHAALGLSQLRKLDRFVLRRKEIAALYDELFEGDRVLCTP
                     PRCEGHAYHLYPLQFSAGNRAAVFEELGKRGIRGMVHYLPVPLHPYYRERFGYVPGEF
                     PHAEELYAREISLPVYYGLTDDDIRRVAEEVRRIADSFPNR"
     gene            880..1323
                     /locus_tag="P5201_09610"
     CDS             880..1323
                     /locus_tag="P5201_09610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013048070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpH family outer membrane protein"
                     /protein_id="HRV98837.1"
                     /translation="MFSGKRIGILLSLALLLICSSAVLASANEKIGVIDSQKIIFQHP
                     RFEAVTKQLQQISQKKENEMKMAVDKESDQNKKAQIFNTKRNEFAQEEAKLMQPIFQE
                     AQVAVRTVARLKGVTVVIEQSAVYFGGIDITDDVIQELKKKAAGK"
     gene            1446..>2141
                     /locus_tag="P5201_09615"
     CDS             1446..>2141
                     /locus_tag="P5201_09615"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR01484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HRV98838.1"
                     /translation="MRMKLLVTDIDETLSRGEQLTPGVISACERLRGNGWTIMVASGR
                     ILATAMPHIRGTGTDLPAILYDGGRIMDTATGRAVSETSMDPALALEIVQAGWEHPVE
                     LQIIGDERAVCRSADAVTRGFFAEAGVPVDSSLESPSVPQEVFRVIFHGIPGSIRSLE
                     RDLKSRFAGRAEIVQAGEGFLDVLPLGVSKGAALRGWLATLSELPEIVVAAGDHHNDM
                     EMLEEADIAVVPED"
BASE COUNT          439 a          562 c          671 g          469 t
ORIGIN      
        1 aaagtgatcg cgccggtgag ctatgccggc taccccgtgg atattgcgcc ctttctcgaa
       61 tccgccgggt cgtgcggcgc tgtggtcgtc gaggatgcct gccatgcgct cggagctgag
      121 cggaacgagg tccgcgtcgg caaggaagcg catatgacgg ccttcagctt ccaccccgtc
      181 aagcacatta cgacgggaga ggggggcatg gtcgctaccg attccgaaga gttcgcccgc
      241 cgtcttcgtc ttttcaggac gcacgggatt gaaaaagagc ctcgtttcat gaccgctccg
      301 ccggacggtc cgtgggcgac ggagatgcag atcctcgggt tcaactaccg tttgagcgaa
      361 ctgcacgccg cgcttgggtt gagccagctc cggaagctcg accgcttcgt ccttcggcgg
      421 aaggagattg cggcgcttta cgacgaactt ttcgaagggg atcgcgttct ttgcacgcct
      481 ccgcgatgcg aggggcatgc ctaccacctc tatccgcttc agttctccgc cggaaaccgc
      541 gccgccgtct tcgaagagct gggaaaacgt gggatccgcg gaatggtgca ttaccttcct
      601 gtgccgctgc acccgtacta cagggagcgg ttcggctacg tccccgggga gtttccgcat
      661 gcggaagagt tgtatgcgcg ggagatcagc ctgcccgtct attacggact taccgacgac
      721 gatattcgca gggttgcgga agaagtccgc aggatagccg atagtttccc gaatcggtag
      781 acggacgagg gattacgtaa atttgcctgg acttttgatt ggtttgggta taaaattttc
      841 tttgtatcta tatttaccga ctgtcgagga ggtagtttaa tgttttcagg aaaacgtatc
      901 ggtattcttc tttcgttggc tcttttgttg atttgttcct ctgctgttct tgcatccgcc
      961 aatgagaaga taggcgtcat agattctcag aagatcattt tccagcatcc gaggttcgaa
     1021 gccgtcacaa agcagcttca gcagatcagc cagaagaaag agaacgagat gaagatggct
     1081 gtggacaagg aatccgatca gaataaaaaa gcgcagatct tcaacacgaa gcgtaacgaa
     1141 ttcgcccagg aagaggcaaa gctgatgcag ccgatcttcc aggaggctca ggtcgcagtg
     1201 aggaccgtgg cgaggttgaa gggcgtcacc gtggtcatcg agcagtccgc cgtctatttc
     1261 ggcggtatcg acatcaccga cgacgtcatc caggagctga agaagaaggc cgcaggaaag
     1321 tagtttttcc gccggttgca gcacagtctg aaagtttgat tttcgaggga gccgcttctc
     1381 tccggtatgc ggagatacaa gcaagcggct ccctttttta ctactgttct tcggaggaaa
     1441 aagagatgag aatgaaactt cttgtaactg atatagacga gacgctttcc cgcggggagc
     1501 agctcacgcc cggtgtgatt tccgcctgcg agcgtctccg gggaaacgga tggacgatca
     1561 tggtagcttc ggggagaatt ctggcaaccg cgatgccgca tatccggggg acggggaccg
     1621 atcttccggc gatcctctac gacggggggc ggatcatgga tactgccacc gggagagctg
     1681 tttccgagac ctccatggat ccggcgctgg cgctggagat agtgcaggcg gggtgggagc
     1741 accccgtgga gcttcagatc atcggcgacg aacgtgccgt ctgccgaagc gccgacgcgg
     1801 tgacccgggg ctttttcgca gaggcgggcg ttcccgtcga ttcctcgctt gaatcgccct
     1861 ccgtccctca ggaggtattc agggtcatct ttcacggcat tcccgggagc atcaggtcgc
     1921 tcgagcggga tctgaagagc cgcttcgccg gaagagcgga gatagtgcaa gcgggcgagg
     1981 ggtttctcga tgttcttcct ctcggggttt ctaagggagc ggcccttcgc ggatggctcg
     2041 cgacgctgtc ggagcttccg gagattgttg tcgcggccgg ggatcaccat aacgacatgg
     2101 agatgctgga agaggccgat atcgccgtcg tgccggagga c
//
LOCUS       DAOTIL010000469         2141 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminobacteriaceae bacterium isolate AMR_MDS_3026
            NODE_61111_length_2141_cov_3.244966, whole genome shotgun sequence.
ACCESSION   DAOTIL010000469 DAOTIL010000000
VERSION     DAOTIL010000469.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868050
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminobacteriaceae bacterium (wastewater metagenome)
  ORGANISM  Aminobacteriaceae bacterium
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae.
REFERENCE   1  (bases 1 to 2141)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2141)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:19:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,224
            CDSs (total)                      :: 3,198
            Genes (coding)                    :: 3,184
            CDSs (with protein)               :: 3,184
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2141
                     /organism="Aminobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_61111_length_2141_cov_3.244966"
                     /isolate="AMR_MDS_3026"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:3034609"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..312)
                     /locus_tag="P5201_09620"
     CDS             complement(<1..312)
                     /locus_tag="P5201_09620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013048025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HRV98839.1"
                     /translation="MQKLDMKSIRNIGIAAHIDAGKTTTTERILFYTGKNYKMGETHE
                     GAATMDYMDQERERGITITSAATTCMWHDHYINIIDTPGHVDFTVEVERSMRVLDGAV
                     AV"
     gene            complement(377..847)
                     /gene="rpsG"
                     /locus_tag="P5201_09625"
     CDS             complement(377..847)
                     /gene="rpsG"
                     /locus_tag="P5201_09625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010720461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S7"
                     /protein_id="HRV98840.1"
                     /translation="MPRKGHVKKRQATPDTKFGSPALGKFINSLMRKGKKSVAERIMY
                     NALDIAGEKLQTEPFEVFKKAMENVRPLVEVRPRRVGGATYQVPVEVPAERAQVLSIR
                     WILNYSRSKKGMPMAERLAREFMDACKNEGASIKKKEDTHKMAEANRAFAHYRW"
     gene            complement(892..1263)
                     /gene="rpsL"
                     /locus_tag="P5201_09630"
     CDS             complement(892..1263)
                     /gene="rpsL"
                     /locus_tag="P5201_09630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012869933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12"
                     /protein_id="HRV98841.1"
                     /translation="MPTINQLIRHGREEKRRKSDSPALQGNPARRGVCTRVYTVTPKK
                     PNSALRKVARVRLTNGIEVTSYIPGIGHNLQEHSVVLVRGGRVKDLPGVRYHIIRGTL
                     DCGGVENRKRSRSKYGARKPK"
     gene            complement(1357..1602)
                     /locus_tag="P5201_09635"
     CDS             complement(1357..1602)
                     /locus_tag="P5201_09635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006301287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal L7Ae/L30e/S12e/Gadd45 family protein"
                     /protein_id="HRV98842.1"
                     /translation="MPLNELAVPNRVTGERQVRRTLAGGRLRKLFIAEDADPSVIKNL
                     EEEARRQNIPVERVDSMLRLGRACAITRGASAAGIEG"
     gene            complement(1730..>2141)
                     /locus_tag="P5201_09640"
     CDS             complement(1730..>2141)
                     /locus_tag="P5201_09640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014806359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRV98843.1"
                     /translation="EVNQTIIEKLSGNAGKMDATGNGIRELVVRPMGGHKKEEKKIIQ
                     RVTFVRKLREGPKVKPFVHGITKAALATDSFLSAASFQQTAQVLAGAAVKGEIDPLEG
                     LKENVIIGHLIPAGTGVERFQGVRTYIQHPEKEE"
BASE COUNT          396 a          644 c          543 g          558 t
ORIGIN      
        1 gaccgctacc gcgccgtcga ggactctcat tgaacgctcg acctcgacag tgaaatccac
       61 gtgcccgggc gtatcaatta tattgatata gtgatcgtgc cacatgcacg tcgtagcagc
      121 ggaggtaatg gtgatccccc gctcccgttc ctggtccatg tagtccatgg tggcggcgcc
      181 ttcgtgcgtt tcgcccattt tgtagttttt tccggtgtag aaaagaattc gctcagtggt
      241 cgtcgtcttg cccgcgtcaa tatgggcggc gattccaatg ttacggatac ttttcatatc
      301 aagtttttgc atttcctacc accaagcctc gtttcctgca gagcactgca tcgtattgaa
      361 tctgtaaacg actttattac cagcggtaat gggcgaacgc gcggttagcc tccgccatct
      421 tgtgcgtatc ttccttcttc ttgatggaag cgccttcgtt cttgcaggca tccatgaact
      481 ccctggcgag tctttcagcc atgggcattc ccttcttgct ccgggaatag ttcaggatcc
      541 atctgatgga gagcacctgc gctctttcgg cgggtacttc caccggtacc tggtaggtcg
      601 ctccgccgac gcggcggggg cgaacctcga cgagaggacg gacgttctcc atggccttct
      661 tgaagacctc gaagggctcc gtctgaagct tctctccggc gatatcgagc gcgttgtaca
      721 taatccgctc ggccacgctc ttctttccct tccgcatcag gctgttgatg aacttaccca
      781 gtgcggggct gccgaacttg gtatccggcg tcgcttggcg tttcttgaca tgccccttac
      841 gcggcattgg tcaacccctc ctcaaaataa caaaaaacac gccgcaaaac cctacttggg
      901 tttccgggcg ccgtacttgg agcgggatct ctttcgattc tccacgcctc cacagtcgag
      961 tgttccccga ataatgtgat accggactcc gggaaggtct ttcactcttc cgccacgaac
     1021 aagaaccacc gagtgctcct gcaggttgtg gccgattccc ggaatgtacg aggtaacctc
     1081 gataccgttg gtgaggcgga ctcgggcaac cttccgcaga gccgagttag gctttttcgg
     1141 cgtcaccgtg tacacacgcg tgcagacacc ccgccgggcg gggtttccct gcagcgcagg
     1201 cgaatcgctc ttgcgcctct tttcttcccg gccatgccgt ataagctgat tgatcgttgg
     1261 cacgcaactt cctccttcca tctgttcttc gtattctggg caaagaaact tttttccgaa
     1321 aaaccctttt ccctcgtctt ccggatattc gcccatctat ccttctattc cagccgccga
     1381 agctcctcgt gtgatggcgc aggctcttcc gagccggagc atactgtcga cgcgttcaac
     1441 cggaatattc tgccgccgcg cttcttcttc gaggtttttt atcactgaag ggtccgcatc
     1501 ctccgctata aagagcttcc ggagccgtcc gccggcaagc gtccgccgta cctgacgttc
     1561 tcccgtcact ctgttcggga ctgcgagttc atttaaaggc aataaaatcc ctcctgtgcc
     1621 aaaagcacca cggaataata acaaaaaggc gggagcaatg tcaagcataa aatgctcccg
     1681 ccttcagagt atttacattt tctcactctg gagcaaagac gccgaagagc tattcttcct
     1741 tttcggggtg ctgaatgtat gtgcgaaccc cctggaatcg ttcaactcct gttccggccg
     1801 gaatgagatg tccgatgatg acgttttcct tcagtccttc aaggggatcg atctctccct
     1861 tcactgcggc tccggcgagt acctgggcgg tctgctggaa cgaggccgcg ctgaggaagc
     1921 tgtcggtggc aagcgccgcc ttcgtgatgc cgtggacgaa gggcttcacc ttcgggcctt
     1981 cgcgcagttt ccggacgaag gtgacccgct ggatgatctt cttttcttct ttcttgtgcc
     2041 ctcccatcgg gcggacaaca agctccctga tcccgtttcc ggtggcatcc atcttgccgg
     2101 cgttgccgga gagcttttct atgatcgtct ggttaacttc c
//
LOCUS       DAOTIL010000470         2139 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminobacteriaceae bacterium isolate AMR_MDS_3026
            NODE_61193_length_2139_cov_2.428983, whole genome shotgun sequence.
ACCESSION   DAOTIL010000470 DAOTIL010000000
VERSION     DAOTIL010000470.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868050
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminobacteriaceae bacterium (wastewater metagenome)
  ORGANISM  Aminobacteriaceae bacterium
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae.
REFERENCE   1  (bases 1 to 2139)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2139)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:19:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,224
            CDSs (total)                      :: 3,198
            Genes (coding)                    :: 3,184
            CDSs (with protein)               :: 3,184
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2139
                     /organism="Aminobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_61193_length_2139_cov_2.428983"
                     /isolate="AMR_MDS_3026"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:3034609"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..575)
                     /locus_tag="P5201_09645"
     CDS             complement(<1..575)
                     /locus_tag="P5201_09645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012870115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HRV98844.1"
                     /translation="MNDHNEQTTIMLVDDTPANLELLRAMLHKKGYKVLVFPGGEPAL
                     RALERKEPDLILLDIMMPEMNGFEVCGRLKEDERLREIPVIFISALDDTQSKVRAFTA
                     GGVDYVTKPFQPEEILARVQTHVTLRQVRRELEKHMSELEELVREKVKEISDSQMATI
                     HAMSKLAEYRDDQTGRHIERTRELCAMLARKL"
     gene            complement(572..>2139)
                     /locus_tag="P5201_09650"
     CDS             complement(572..>2139)
                     /locus_tag="P5201_09650"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HRV98845.1"
                     /translation="NRWFPKKSTTSAVERRLWIASGVFLAGLLLSLTMLAALRWQVQR
                     RTFELALAKEEAEKANKAKSSFLANMSHEIRTPLNAILGFGAVLERDPSLSPKQAEYV
                     NAVSRSGRHLLKLINDILDMSRIEADRLVLNPTDFNLRDLLADMRMMFGARAEEKHLQ
                     LIMEIQETLPVYARGDEAKVRQIFVNLLGNAVKFTLSGGVAVRARMDKPREAESASFL
                     LVEVEDTGPGIPPEDMKKIFDPFCQSDAGKQAGGSGLGLSISKRLAELMGGALTASST
                     VGKGTCFRLRLPLHTAEHTAEHPRTALRSIVGAESASSYRILLVDDIKENRDVLRAVL
                     EPLGFSLMEAVDGAEGVKLFEEWTPHAVLMDMRMPVMDGYEATRRIKETPEGVAVPVI
                     AITASAFEDEVQNVLNSGADYCIRKPLRPEELFEILGEALGLRYVYGADTAEHEEKPH
                     EITAGDVARLPESLTSAMRTAVEEGDILQLEALINTIQEVDAPLADGLRRLARRYDYE
                     TLTDLLPEEVESP"
BASE COUNT          350 a          654 c          652 g          483 t
ORIGIN      
        1 agtttccggg cgagcatggc gcagagttcg cgcgtccgct cgatgtgccg gccggtctgg
       61 tcgtcccggt actcggccag cttcgacatc gcatgaatcg tcgccatctg cgagtcggag
      121 atctccttca ccttctccct gacaagctct tcgagttccg acatgtgttt ttcaagctcg
      181 cggcgcacct gccgcagcgt aacgtgcgtc tgcactctcg ccaggatctc ctccggctga
      241 aacggtttgg tcacgtagtc cacgcctccg gcggtaaagg ccctcacctt gctttgcgtg
      301 tcgtccagcg cgctgatgaa gatcacggga atttcccgga ggcgttcatc ctctttcagc
      361 cggccgcata cttcaaaacc gttcatctcc ggcatcatga tgtcgagcag aatcagatcg
      421 ggttctttcc tttcgagcgc gcggagcgcc ggttctccgc cggggaagac cagcaccttg
      481 tacccttttt tgtgcagcat cgcccgaaga agttcgaggt tggccggggt atcgtcgacc
      541 agcatgatgg tcgtctgttc gttgtgatcg ttcacgggga ttccacctcc tccggtaaaa
      601 gatctgtgag cgtttcgtag tcgtatctcc gggcaagacg ccgcagtccg tccgcaagcg
      661 gcgcgtcgac ttcctgaatc gtgttgatca acgcttccag ctggaggatg tccccctcct
      721 cgacggcggt tctcatggcg gacgtcagcg attcgggcag ccgcgccacg tccccggccg
      781 tgatttcatg gggcttctct tcgtgttcgg cggtgtcggc tccgtagacg tagcgcagtc
      841 cgagggcctc gccgagtatc tcgaacagct cttccggtcg gagaggtttg cgtatgcagt
      901 agtccgctcc gctgttcagg acattctgca cctcgtcttc aaacgcgctc gctgtgattg
      961 cgatgaccgg gactgcgacg ccctcagggg tctccttgat gcggcgcgtc gcctcgtagc
     1021 cgtccataac gggcatgcgc atatccatca gcacggcgtg cggcgtccat tcttcgaaga
     1081 gtttcacccc ctcggccccg tcgaccgctt ccatcaggct gaagcccagg ggttcgagaa
     1141 cggcgcgcag tacgtccctg ttttctttga tgtcgtcgac cagcaatatc cggtaggaag
     1201 aggcggattc agcgccgacg atgctgcgaa gggctgtccg cggatgttcc gccgtgtgtt
     1261 ccgccgtgtg caaaggaagg cgcaggcgga agcacgttcc ttttccaacg gtgctcgagg
     1321 cggtgagcgc gccgcccata agctccgcaa ggcgtttgct gatggacagt cccagtcccg
     1381 aacctcccgc ctgttttccg gcgtccgatt gacagaaggg gtcgaagatc ttcttcatgt
     1441 cctcaggggg aatgcccggt ccggtgtcct cgacctcgac gaggaggaag gaagcgcttt
     1501 ccgcttcgcg cggcttgtcc attctggcgc gtaccgcgac gccgccggag agcgtgaatt
     1561 tcacggcgtt tccgaggagg ttgacgaaaa tctgccgtac cttggcctcg tcgcccctcg
     1621 cgtacactgg aagagtctcc tgaatctcca tgatgagctg caaatgtttt tcctcggcgc
     1681 gggcgccgaa catcatccgc atgtcggcca ggagatcgcg aaggttgaag tcggtcgggt
     1741 tgagcacgag ccggtcggct tcgatcctcg acatgtcgag gatatcgttg atcagcttca
     1801 gaagatgccg cccgcttcgg ctgactgcgt tcacgtactc ggcctgtttt ggagagagcg
     1861 acggatcccg ttcgagcacc gcgccgaagc cgaggatggc gttgagcggc gtgcggatct
     1921 cgtggctcat attggcgagg aaggagctct tggccttgtt ggccttctcc gcctcctctt
     1981 tggccagggc aagttcgaag gtgcgtcgct gtacctgcca gcggagcgcc gcgagcattg
     2041 tcaggctgag aagcagtccc gccaggaaaa ctccggaggc gatccacagc ctgcgctcca
     2101 ccgcgcttgt ggtggatttt ttggggaacc agcggttca
//
LOCUS       DAOTIL010000471         2136 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminobacteriaceae bacterium isolate AMR_MDS_3026
            NODE_61311_length_2136_cov_2.982701, whole genome shotgun sequence.
ACCESSION   DAOTIL010000471 DAOTIL010000000
VERSION     DAOTIL010000471.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868050
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminobacteriaceae bacterium (wastewater metagenome)
  ORGANISM  Aminobacteriaceae bacterium
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae.
REFERENCE   1  (bases 1 to 2136)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2136)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:19:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,224
            CDSs (total)                      :: 3,198
            Genes (coding)                    :: 3,184
            CDSs (with protein)               :: 3,184
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2136
                     /organism="Aminobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_61311_length_2136_cov_2.982701"
                     /isolate="AMR_MDS_3026"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:3034609"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(115..948)
                     /gene="cobM"
                     /locus_tag="P5201_09655"
     CDS             complement(115..948)
                     /gene="cobM"
                     /locus_tag="P5201_09655"
                     /EC_number="2.1.1.133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012868806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0046026 - precorrin-4
                     C11-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="precorrin-4 C(11)-methyltransferase"
                     /protein_id="HRV98846.1"
                     /translation="MANTEYPVLFVGAGPGDPELITLKGKRALEEADLVLYAGSLVNE
                     ALLDYCSPGCVTADSSPLSLEEQMALVAAKVREGGRVVRLHTGDPSLFGAVAEQKERL
                     ESLGIEVRFIPGVSSFQAAAAALGIQYTVPGGSQTVVCTRRSGRTPVPPRENLRLLAE
                     HGATMVVFLSADQAEEVSADLQAGGFSAETPAACVYRASWEDEKTIVSTLGALPSLMA
                     EHGITRHALIVVGECVASHGARSLLYSPHFAHGARDAAPEQSDEERTLASSEGFCEAP
                     L"
     gene            complement(999..>2136)
                     /gene="cbiE"
                     /locus_tag="P5201_09660"
     CDS             complement(999..>2136)
                     /gene="cbiE"
                     /locus_tag="P5201_09660"
                     /inference="COORDINATES: protein motif:HMM:TIGR02467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046025 - precorrin-6Y
                     C5,15-methyltransferase (decarboxylating) activity
                     [Evidence IEA];
                     GO_process: GO:0046140 - corrin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="precorrin-6y C5,15-methyltransferase
                     (decarboxylating) subunit CbiE"
                     /protein_id="HRV98847.1"
                     /translation="LLEKGVDDLLDAVDEHRKHGRVALLLSGDPCLFSLLGRIAGRFP
                     SGDYEVVPGLSSFQLLFARLASLPGEPGAPSPSWSDVRLESIHGRPIEGVASMLHEHR
                     RTLLFLDSKNSGPAVAAFLRDRGFPDRRVILAERVGYENERISPTTLFNLANEQDAEG
                     LALLLVEPGPIPPLSKGVLPDSWFVRHERTPLSKEITRALTVSFLLPLDGLSVLEVGT
                     GSGGITVELARRIAGGNVVSVERSPDALAVAEENLRRAGVFDRVRLLRGSAPEVFAEL
                     NDRSGAPLRFHRAVIGGHGGASGEIATAAWNRLLPGGRLLATANMPSSADAIYRALKS
                     LGATPSLTHVNASSARESAGQGAEASWMLLASNPVFLIYADRNA"
BASE COUNT          354 a          708 c          724 g          350 t
ORIGIN      
        1 aggaggcgac cgtcgcggcg agggctccgg actcgacgcg gacgacggtc cccgcctctt
       61 caagacggcg ggccgtcgct tctcccttcg acgagaagca gaaaaacgcc gtcttcatag
      121 cggagcctcg cagaagcctt cggacgaagc gagcgttcgt tcttcgtccg actgttcggg
      181 agcggcgtcc cgcgcgccgt gggcgaagtg cggcgagtag agcaggcttc gtgcgccgtg
      241 cgacgcgacg cactctccga cgacgatcag cgcgtggcgg gtgatgccgt gctccgccat
      301 cagcgaaggg agcgcgccga gcgtggagac gatcgtcttc tcatcctccc aggaggcgcg
      361 gtagacgcag gcggcgggtg tttcggcgga gaatcccccc gcttggaggt ctgcggagac
      421 ctcctccgcc tggtccgcgc tgagaaagac caccatcgtc gcgccgtgct ccgccagcag
      481 gcggagattc tcgcggggag gcacgggcgt ccgtcccgat ctgcgggtgc agacgaccgt
      541 ctggcttcct ccggggacgg tgtactgaat gcccagtgcg gcggcggcgg cctgaaagct
      601 gctcactccg gggatgaacc ggacctcgat gccaagcgat tccagccgct ccttctgctc
      661 ggccacggcc ccgaagaggc tcgggtcgcc ggtgtgcagt cgcacgacgc gcccgccttc
      721 cctgactttt gccgccacca gcgccatctg ctcttcgagc gaaagcggcg acgagtcggc
      781 cgtaacgcag ccgggggagc agtagtcgag gagcgcttcg ttgacgaggc tccccgcgta
      841 gagcaccagg tccgcctctt cgagcgcgcg cttccctttg agtgtgatca gttccgggtc
      901 acccggaccc gcgccgacga agagcacggg gtattctgta ttcgccattg gttttccctc
      961 actttgtgtg ttccagtgcg cgttctcccg tatacccgct acgcgttcct gtcggcgtag
     1021 atcaaaaaga ccgggttcga ggcgagcagc atccacgacg cctccgctcc ctgtccggcg
     1081 ctctcccgcg ccgacgaggc gttcacatgc gtcagcgacg gcgtcgcgcc caggctcttc
     1141 agcgcgcggt agatcgcgtc ggctgaagag ggcatgttcg ccgtcgccag cagcctccct
     1201 ccgggaagaa gacgattcca cgcggctgtg gcgatctcgc ctgaggcgcc tccgtgacct
     1261 ccgattacgg cacggtggaa acggagcggc gcgcccgagc ggtcgttgag ctctgcaaac
     1321 acctcgggag cggagccgcg gagcaggcgg accctgtcga aaaccccggc gcggcgcaga
     1381 ttctcctccg cgacggcgag ggcgtccggc gagcgttcca ccgaaacgac gtttcctccc
     1441 gctatccggc gtgcgagttc cacggtgatc ccccccgaac ccgttcccac ttcgagcacg
     1501 gagagcccgt cgagagggag cagaaaggag acggtaaggg cccgcgtgat ctccttcgag
     1561 aggggggtgc gttcgtgccg gacaaaccag gagtccggaa ggaccccttt ggagagcggc
     1621 gggatcggtc ccggctcgac caggagcagc gcaagcccct ccgcgtcctg ttcgtttgcg
     1681 aggttgaaga gcgtggtcgg ggagatgcgt tcgttctcgt accccaccct ctcggcaagg
     1741 atgacgcgcc ggtcgggaaa gcccctgtcc cgcagaaacg ccgccacggc agggccggag
     1801 ttcttcgagt cgaggaagag cagcgttctc ctgtgctcat ggagcatcga ggcgacccct
     1861 tcgatggggc gtccgtggat gctttcaaga cgcacatcgc tccacgaggg gctcggagcg
     1921 cccggttccc ccggaagcga cgcgagtcgg gcgaaaagga gctggaagct gctcaggccg
     1981 ggaacgacct cgtagtcgcc ggaggggaaa cgacccgcga tccgcccgag gaggctgaaa
     2041 aggcaggggt ctcctgaaag cagcagagcg acccgtccgt gtttccggtg ttcgtccacc
     2101 gcgtcgagga ggtcgtccac gcccttttcc agcaac
//
LOCUS       DAOTIL010000472         2132 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminobacteriaceae bacterium isolate AMR_MDS_3026
            NODE_61477_length_2132_cov_3.044776, whole genome shotgun sequence.
ACCESSION   DAOTIL010000472 DAOTIL010000000
VERSION     DAOTIL010000472.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868050
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminobacteriaceae bacterium (wastewater metagenome)
  ORGANISM  Aminobacteriaceae bacterium
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae.
REFERENCE   1  (bases 1 to 2132)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2132)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:19:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,224
            CDSs (total)                      :: 3,198
            Genes (coding)                    :: 3,184
            CDSs (with protein)               :: 3,184
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2132
                     /organism="Aminobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_61477_length_2132_cov_3.044776"
                     /isolate="AMR_MDS_3026"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:3034609"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>2132
                     /gene="fdhF"
                     /locus_tag="P5201_09665"
     CDS             <1..>2132
                     /gene="fdhF"
                     /locus_tag="P5201_09665"
                     /EC_number="1.17.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006299821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009326 - formate dehydrogenase complex
                     [Evidence IEA];
                     GO_function: GO:0008863 - formate dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_process: GO:0015942 - formate metabolic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_except=(pos:603..605,aa:Sec)
                     /transl_table=11
                     /product="formate dehydrogenase subunit alpha"
                     /protein_id="HRV98848.1"
                     /translation="VCEEHAIYHAIDFQGRGIHTSIQPPVGSPIEESDCVFCGQCVQV
                     CPVGALFEKTAAGQGRPWDLEKVKTVCSYCGVGCELEVNVNKKTGKIANVTTNYSSPT
                     ALNKGRSCVKGRFAWQFVHSEDRLTTPLIREGDTFRKASWDEALSLVAEKLGEVKTKH
                     GSDALGFFSSARCTNEENYLMQRLAREVMGTNNVDHCAHLUHSATVTGLGETLGSGAM
                     TNDFESIKSADTILIIGSNTTENHPVIGSFIKERKRKGNCTLIVCDPRKIEMAKYADV
                     YIRQKSGSDVVLLNGLMHVILRENLYASDFIEKNVNNFEKLKETMSKYTPQYVSEITG
                     VSPELIEDAARAYAKGPNSAIFYTMGITQHLNGTDNVRTVANLALLCGMLGRPGTGVN
                     PLRGQNNVQGACDTGCLPEVIPGYQKVTLPATQQKVKELWGSDLPATPGLSVVEMMHA
                     AGEGKIKGMYIMGENSMVTEPDSDHVRHALKKLDFLVVQDIFLTETAEFAHVVLPAAS
                     WGEKDGTFTNTCRAVQRVRKAVDSPGEARPDWEILLAVGKRLGASWNDMNSPETIFNA
                     IGRIAPAYAGITYARLEGGALPWPCPTCEHPGTPILYTTGFPRGKADFSPCDWVKPDE
                     WPTEEYPFIATTGRILYHYHSGSMTRRSSTGEFIKEMYIEMNPADATSLNVKDGEQIK
                     VTSRRGEVSGSVKITDRVPAGTVFLPFH"
BASE COUNT          517 a          597 c          582 g          436 t
ORIGIN      
        1 gtgtgtgcga agaacacgcc atctaccacg ccatcgattt ccaggggcgt ggaatccaca
       61 cctccatcca gcctcctgtg ggttcgccca tagaggagtc cgactgcgtc ttctgcgggc
      121 agtgcgtcca ggtctgccct gtaggagccc tcttcgaaaa aacagcggca ggacaggggc
      181 gtccgtggga tcttgaaaag gtcaagactg tctgttccta ctgcggcgtt ggatgcgagc
      241 tcgaggtcaa cgtcaacaag aagacaggca aaatcgccaa tgtcaccacg aattactcca
      301 gtccgacagc tttgaacaag ggaagatcgt gcgtgaaggg acgcttcgca tggcagtttg
      361 tccacagcga ggaccgcctg accaccccgc ttattcgtga aggcgacacc ttccggaaag
      421 cgtcctggga cgaagcgctc tctcttgtgg ctgaaaagct cggggaagta aagacaaaac
      481 acggctcgga cgcgcttgga tttttctcct cagcccgttg caccaacgag gagaactatc
      541 ttatgcagcg ccttgcaagg gaggtgatgg gcactaacaa cgtcgatcac tgtgcccatc
      601 tgtgacacag cgccacagtg acgggtctcg gcgagaccct cggcagcggc gctatgacga
      661 acgactttga atctatcaaa tctgccgata cgattcttat tatcggctcg aacacgactg
      721 aaaaccaccc tgtcatcggc tctttcataa aggaacgcaa aagaaaaggg aactgcaccc
      781 tgatcgtgtg cgatccccga aagatagaaa tggcaaagta cgcagacgtt tatatacgcc
      841 agaagagcgg atcggacgtt gtacttctga atggtttgat gcacgttata cttcgcgaga
      901 acctctacgc ttccgatttt attgaaaaga acgtgaataa ctttgagaag ctgaaggaaa
      961 ccatgtccaa gtacaccccg cagtacgttt cggaaattac cggcgtttcc cccgagttga
     1021 ttgaggatgc cgcacgtgcg tacgcgaaag ggccaaactc cgccatcttc tatacgatgg
     1081 gcattacgca gcatcttaac ggtactgata acgtccggac cgtcgcgaac cttgcgcttc
     1141 tctgcgggat gctgggacgc cccggaacag gagtcaaccc gttgcgtgga cagaacaatg
     1201 tgcagggagc ctgcgatacc ggatgccttc cggaagtgat ccccggatac cagaaagtca
     1261 cccttcctgc gacgcagcag aaggtcaagg agctctgggg ttctgatctc cctgcgactc
     1321 cgggcctttc cgttgtcgag atgatgcatg ccgccggcga aggaaaaatc aaaggcatgt
     1381 acatcatggg cgagaactcg atggtcactg aacccgactc tgaccacgtc cggcatgccc
     1441 tgaaaaaact cgattttctg gtagtgcagg acatcttcct tacggagacg gcagagttcg
     1501 cacacgttgt tcttccggcc gcgagctggg gcgagaagga tggaacgttt accaacactt
     1561 gccgcgcagt acagagagtc cgcaaagctg tcgactctcc cggcgaggcc cgccctgact
     1621 gggagatcct gcttgcggta ggtaaacgac tcggcgcttc atggaacgat atgaattcac
     1681 cggagacaat ttttaacgcc atcggccgta tagctcccgc atatgctgga ataacctatg
     1741 cccgccttga agggggcgct cttccgtggc catgcccgac gtgcgaacac cccgggacgc
     1801 cgattctgta cacaacgggt ttccccagag gcaaggctga cttctctccg tgcgattggg
     1861 taaaacctga tgaatggccg acagaagagt atccgttcat tgcaactacc ggcaggatcc
     1921 tctaccacta ccactccgga agcatgaccc gccggagctc gaccggcgag tttatcaagg
     1981 agatgtacat cgagatgaat ccggccgacg ccacttcgct gaacgtcaag gatggagaac
     2041 agatcaaggt tacttcccgc agaggagaag tcagcggttc agtgaagatt accgatcgcg
     2101 tgcccgctgg gaccgtcttc cttccgttcc ac
//
LOCUS       DAOTIL010000473         2131 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminobacteriaceae bacterium isolate AMR_MDS_3026
            NODE_61504_length_2131_cov_3.695568, whole genome shotgun sequence.
ACCESSION   DAOTIL010000473 DAOTIL010000000
VERSION     DAOTIL010000473.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868050
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminobacteriaceae bacterium (wastewater metagenome)
  ORGANISM  Aminobacteriaceae bacterium
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae.
REFERENCE   1  (bases 1 to 2131)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2131)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:19:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,224
            CDSs (total)                      :: 3,198
            Genes (coding)                    :: 3,184
            CDSs (with protein)               :: 3,184
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2131
                     /organism="Aminobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_61504_length_2131_cov_3.695568"
                     /isolate="AMR_MDS_3026"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:3034609"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1002)
                     /locus_tag="P5201_09670"
     CDS             complement(<1..1002)
                     /locus_tag="P5201_09670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013047653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HRV98849.1"
                     /translation="MKLSIKVEPRLETPRMLGIAIPVISLAAALLSGAVFLFFMGVSP
                     FQAYSTIIRSSLGDAYGLSETLVKAIPLAMAAIAVMLSFTMLIWNIGAEGQIFMGAIA
                     AAAAVRYLFVDNHLAMFAIMFAAAGIAGGIWAAIAGYFRARWSVNEIITTLMLNYVAM
                     NLLNYFVYGPWRDPASLGFPMTAPFPDAARLPSFWGTRIHAGLFLAILFPLIFWVILR
                     FTRWGYEIRVMGENPKAAGFAGMSYIKNVVFVMFLSGAIAGIAGMCELSGLQGRLQHG
                     FSGGYGYTAIIVAWLARLHPIAIILVSFLMGVLLVGGETLQIVMRLPLSSMLVLQGLI
                     "
     gene            complement(999..>2131)
                     /locus_tag="P5201_09675"
     CDS             complement(999..>2131)
                     /locus_tag="P5201_09675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005661475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HRV98850.1"
                     /translation="SIGEQQRVAILQMLYRRAKILVLDEPTAVLTPQEAEHLFVTVRQ
                     MTSEGHGIVFISHKMDEVMGLSHRVTILRKGRLVGTVDTASTTKEKLAEMMIGQKMIF
                     SIEKKELQPGKPVIECASVCALGDRGIPAVSRATFMIREQEILGVAGVAGNGQQELCE
                     TLVGLRKVESGAIRVNNEELTNSIPKTFIDKGIHYIPADRKGTGLVPNMNVSENSILK
                     RYWSRAVSKKGFIDWKEVFSFARGLVSRYNVSTPSMETPVRNLSGGNLQKLMLGRELS
                     DSPIAILAVHPTWGLDVAATKFVREQLLAAREAGCAVLLVSEDLDELLAMSDRLAVMC
                     KGEIMGILDSPAETPVEAIGLMMAGTPAEEALASAPRGGEKS"
BASE COUNT          479 a          603 c          622 g          427 t
ORIGIN      
        1 gatcaaacct tgcagcacga gcatgcttga aaggggaagc cgcatgacga tctgaagagt
       61 ctcgccgcct accaggagca cccccatgag gaaggagacc agtatgattg cgatcgggtg
      121 caggcgggcc agccatgcca cgataatcgc ggtgtagccg tagccgccgg aaaaaccgtg
      181 ctgtaagcgg ccctgcagcc cggagagttc gcacatcccc gctattccgg cgatagctcc
      241 ggaaagaaac atcacgaaca ccacgttttt aatatagctc atccctgcaa aaccggcagc
      301 tttgggattt tctcccatga cccgaatctc gtacccccac ctggtgaaac ggagaatcac
      361 ccagaagatc agaggaaaca ggattgcgag gaaaagccct gcatggattc gggtgcccca
      421 gaaagagggg agacgagccg cgtccggaaa aggggccgtc atcgggaagc cgaggcttgc
      481 agggtcgcgc cacgggccgt agacaaaata gttcagcagg ttcatggcca cataattgag
      541 catcagcgtc gtgatgatct cgttgacgct ccatcgcgcg cggaagtatc cggcgatggc
      601 cgcccagatt ccccctgcga ttcccgctgc cgcaaacatg atggcgaaca tcgccaggtg
      661 gttgtcgacg aagaggtagc gaacagcggc ggcggcggca atagctccca tgaagatctg
      721 cccctccgcg ccgatattcc aaatgagcat tgtgaaggag agcatcaccg cgatcgcagc
      781 cattgcaaga gggatcgcct tgacgagagt ctcgctgaga ccgtacgcgt cacccaaaga
      841 agagcggatg atggtggagt aggcctggaa cggagagacg cccatgaaga agagaaaaac
      901 agctcccgaa agaagcgcgg ctgcgagtga gatgacggga atggcaatgc cgagcatccg
      961 cggggtttca agtcggggtt ccactttgat agagagtttc atgatttttc tcctcctcgg
     1021 ggagcggagg caagcgcctc ttcagcgggt gttccggcca tcataaggcc gatggcttcg
     1081 acaggcgtct ccgcaggcga atccagaatg cccatgatct cgcccttgca cataacggca
     1141 aggcggtcgc tcatggcgag gagttcgtcg agatcctccg aaaccagcag aacggcgcat
     1201 ccagcctcgc gggcggcgag aagctgttcc ctgacgaact tcgtcgccgc gacgtcgagt
     1261 ccccatgtgg gatgaacggc gaggatggct atcggggagt cgctcagttc gcgtccgagc
     1321 atcagcttct ggaggttccc tccggagagg ttgcgaacag gagtttccat cgaaggagtg
     1381 ctcacattgt agcgggaaac cagccctctg gcgaaagaga agacttcttt ccagtcgatg
     1441 aatcccttct tggaaacggc gcggctccag tatctcttca ggatggagtt ctcgctcaca
     1501 ttcatattgg gaacgagccc ggttcctttc cggtcggcgg ggatatagtg tatccctttg
     1561 tcgatgaagg tcttcggaat cgaattggtc agctcctcgt tattgactcg aattgcaccg
     1621 gactcaacct ttcgaagtcc caccagtgtt tcgcaaagct cctgctggcc gtttcccgcc
     1681 actcctgcga cgcccaggat ttcctgctcc cggatcatga acgtcgcgcg cgatacggcg
     1741 ggaatgcccc ggtcgccgag ggcgcagacg gaagcgcatt caataaccgg tttcccgggc
     1801 tgaagctctt tcttctcaat actgaagatc atcttctggc cgatcatcat ctccgccagc
     1861 ttttcctttg ttgtggacgc cgtatcgaca gttcctacaa ggcggccttt ccgtaggatc
     1921 gtcacccgat gggaaagccc catgacctca tccattttgt gagagatgaa gacgatgccg
     1981 tgtccttcgg atgtcatctg gcgtacagtc acgaagagat gctccgcctc ttgaggtgtg
     2041 agcaccgccg tgggctcgtc gagcacaagg atttttgccc tcctgtagag catctggaga
     2101 atcgccacac gttgctgttc accgatggag a
//
LOCUS       DAOTIL010000474         2130 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminobacteriaceae bacterium isolate AMR_MDS_3026
            NODE_61553_length_2130_cov_3.511325, whole genome shotgun sequence.
ACCESSION   DAOTIL010000474 DAOTIL010000000
VERSION     DAOTIL010000474.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868050
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminobacteriaceae bacterium (wastewater metagenome)
  ORGANISM  Aminobacteriaceae bacterium
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae.
REFERENCE   1  (bases 1 to 2130)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2130)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:19:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,224
            CDSs (total)                      :: 3,198
            Genes (coding)                    :: 3,184
            CDSs (with protein)               :: 3,184
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2130
                     /organism="Aminobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_61553_length_2130_cov_3.511325"
                     /isolate="AMR_MDS_3026"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:3034609"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..869
                     /locus_tag="P5201_09680"
     CDS             <1..869
                     /locus_tag="P5201_09680"
                     /inference="COORDINATES: protein motif:HMM:NF021622.2"
                     /note="McrC protein together with McrB forms the McrBC
                     restriction system; recognizes N4- and C5-methylcytosine
                     (and 5-hydroxy-methylcytosines); appears to act against
                     5-methylcytosine preceded by a purine residue; MrcC
                     modulates the specificty of McrB and has DNA cleavage
                     activity; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="5-methylcytosine-specific restriction
                     endonuclease system specificity protein McrC"
                     /protein_id="HRV98851.1"
                     /translation="YLSNKEAISSLKGKLIVSESVKRHTLLKKRMICQFDVFSEDTPM
                     NRILKTAMTLLMRHGDVKKENSGLLRKLLLYFSDVSPLSPYGIDWNALTYHRHNATYK
                     MLLNICRLVFDGLLLSSKDGGCTLAEFIDDQQMHRLFEKFVLEYYRKEYPQFKASASH
                     IDWNIDGADSTLFLPLMKSDITLTHAGRSMIIDTKYYSRSMQSNPQFQSTSLISGNLY
                     QIFTYVKNKDREVMGNVSGVLLYAKTDETVTPDCDYRICGNRIAVKTLNLGAEWLEIK
                     RQLDDLTLYITE"
     gene            1776..2003
                     /locus_tag="P5201_09685"
     CDS             1776..2003
                     /locus_tag="P5201_09685"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRV98852.1"
                     /translation="MLGAIIGDIAGSRFEFNNHRSKEFELFTDKCFATDDSIMTLAVA
                     EAIMEAERSAGSADRDDAFYSALGGLTVRYR"
BASE COUNT          523 a          573 c          610 g          424 t
ORIGIN      
        1 attacctttc caataaggaa gcgataagca gtttgaaagg gaagctgatc gtctcggagt
       61 ccgtcaagcg ccatacgctc ttgaaaaaac gcatgatctg ccaattcgac gtcttctccg
      121 aggatacgcc gatgaaccgg atactgaaga cggcgatgac gctcctcatg cggcacggcg
      181 acgtgaaaaa agagaacagc ggccttttga gaaagctgct tctctacttt tcggacgttt
      241 cccctctctc cccgtacggc atcgactgga acgcgctgac ataccacagg cacaacgcga
      301 catacaagat gctgctgaac atctgccgtc tggtgttcga cggcctgctg ctctcaagca
      361 aagacggagg ctgcacactg gccgaattca tcgacgatca gcagatgcac cgtcttttcg
      421 agaagttcgt cctcgagtac tacaggaagg agtatccgca gttcaaggcc tccgcttccc
      481 acatcgactg gaacatcgac ggcgcggaca gcacactctt ccttccgctg atgaaatccg
      541 atatcaccct gacgcatgcg ggcagatcga tgatcatcga cacaaagtac tacagccgca
      601 gtatgcagtc gaatcctcag tttcaaagca cgtcgctgat ctcgggcaac ctgtaccaga
      661 tcttcaccta cgtaaaaaac aaggatcgcg aggtgatggg caacgtcagc ggcgtactgc
      721 tctacgccaa aacggacgaa acagtcacac cggactgcga ctaccggatt tgcggcaaca
      781 gaatagccgt gaagacattg aacctcggtg cggagtggct tgaaatcaag agacagctcg
      841 acgatttgac gctgtatatc acagagtgag cctgtacgca cgactatata aaaaagctgg
      901 aagaaggatg aatcaataag cggaggacat ttcgaccatc aggagtaccg cttcatcgat
      961 atcgcggagc agttggaacg ggatatcgag tataacgacg tttcatggga gaatccggtg
     1021 gtgacggaat accgcaggat atacggcttt cagttggagc ccgcgacctt ggactatctt
     1081 cggaaagtcg ccgcccaact gcgctatctc cagatggtct tgcacgaata cgacttggcg
     1141 tctccaagga tacatgcgag aagacattcc agcagcgcgt cggcatcgct gcggagacga
     1201 agtcgggatc aggtcgggac taagtcggga ccaagtcggg accaagtcgt cgctctcaca
     1261 ctatgcgggc cgctcttgcg attgcaatgc cgctaccgag cagggtgatt ctcacctgac
     1321 cgtggaagtc gccggggaag taccggggta gttgccggga aagtaccgga gagtttccgg
     1381 ttcatctttc caaagacgat aacaggagca ggaggagatg gcggatgctt cggcagttgc
     1441 gaatacaaaa cttcaaggag tgcaaagata ccagactcat ccgcatggct ccgatatctt
     1501 tgttcttcgg ttcaagaggg aagatggatc ccccatgaac gacttcttca agttcccctc
     1561 tactcctcat ctggctgttc tgctgggtgt cgacgtccgg ggggacaagg tgatgtccga
     1621 gaaggagcgg gacgaattcc tacggcacga gatcaccgtt gaagagaaga tcgacggggc
     1681 caaccttggc atttcgttcg acggcggcgg gagcctcctg ctgcagaaca ggggaggcat
     1741 cgttcaacta cataccataa aggaggcgac gccccatgct cggcgcgatc atcggagata
     1801 tcgcgggttc acgattcgag ttcaacaacc acaggagcaa ggagttcgag ctgtttacgg
     1861 acaagtgttt cgctacggac gacagcatca tgacgctcgc cgtggccgag gcgatcatgg
     1921 aggccgagag gagcgccggc tccgcggacc gtgacgacgc gttctactcc gctctcggcg
     1981 gcctgacggt gaggtatcgc tgaggcctac tacggcgttc cggccgatct gcgggagcgg
     2041 gcgctgcgtt acctcgacaa ggatctgcgg gcgatctacg acgagtggac cgccttttcg
     2101 gcggaagagt gaacggttcc acgcttccgg
//
LOCUS       DAOTIL010000475         2130 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminobacteriaceae bacterium isolate AMR_MDS_3026
            NODE_61561_length_2130_cov_3.003373, whole genome shotgun sequence.
ACCESSION   DAOTIL010000475 DAOTIL010000000
VERSION     DAOTIL010000475.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868050
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminobacteriaceae bacterium (wastewater metagenome)
  ORGANISM  Aminobacteriaceae bacterium
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae.
REFERENCE   1  (bases 1 to 2130)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2130)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:19:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,224
            CDSs (total)                      :: 3,198
            Genes (coding)                    :: 3,184
            CDSs (with protein)               :: 3,184
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2130
                     /organism="Aminobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_61561_length_2130_cov_3.003373"
                     /isolate="AMR_MDS_3026"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:3034609"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(43..522)
                     /locus_tag="P5201_09690"
     CDS             complement(43..522)
                     /locus_tag="P5201_09690"
                     /inference="COORDINATES: protein motif:HMM:NF015060.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan-rich sensory protein"
                     /protein_id="HRV98853.1"
                     /translation="MSKSIGLLFWLGIVFAAAAVGGLASVESASFYKELARPSWAPPG
                     WLFGPVWTALYAMMGVAAWLVWRERGFRGGLVPLSLFIDQLVLNALWTWIFFVWRQGM
                     WAFVEILVLWGLIVATMKAFHAVRPLAAWLLAPYLAWTTFAAALAFTIWRMNPVLLG"
     gene            903..1229
                     /locus_tag="P5201_09695"
     CDS             903..1229
                     /locus_tag="P5201_09695"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRV98854.1"
                     /translation="MEAATPYETILYLARKYIWWKTPEEAAAMPERVLAQVMNIGDYD
                     DVLLMKSRFEDDALRNVIAHAEAGQFNARSWHYWHYMLGLAEIDHVPPLPTRRFSWRG
                     AGKTTS"
     gene            complement(1219..1506)
                     /locus_tag="P5201_09700"
     CDS             complement(1219..1506)
                     /locus_tag="P5201_09700"
                     /inference="COORDINATES: protein motif:HMM:TIGR02385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system YafQ family
                     toxin"
                     /protein_id="HRV98855.1"
                     /translation="MTLRYEIQRTSRFKRDFALVERRGCDISLLADVIRALASGETLP
                     RQERDHTLSGDYAEYRECHIRPDWLLIYKITEESLVLTLERTGTHSDLFKK"
     gene            complement(1503..1733)
                     /locus_tag="P5201_09705"
     CDS             complement(1503..1733)
                     /locus_tag="P5201_09705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006301515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRV98856.1"
                     /translation="MPRTTLEISAKKELYRRFRKLSERNAVRVLGYIDALEEERPNEE
                     TVAALEEGERMLKDPDALRFSTVEDLLTELRS"
     gene            1864..2064
                     /locus_tag="P5201_09710"
     CDS             1864..2064
                     /locus_tag="P5201_09710"
                     /inference="COORDINATES: protein motif:HMM:NF027249.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicB family
                     antitoxin"
                     /protein_id="HRV98857.1"
                     /translation="MTEFYAVIDRDEDGQFFAYVPELPGCHTCGETMEELLENLEEAI
                     ALYLEVVEYPREFPSIRKVAVG"
BASE COUNT          446 a          643 c          620 g          421 t
ORIGIN      
        1 tcaataatgg cgttcgtgtt tataacatgc ttcgcgcgct cctcacccca gcaggacggg
       61 gttcatcctc cagatcgtga aggcgagcgc ggcggcgaag gtcgtccacg cgaggtacgg
      121 agccagcagc cacgcggcga gcgggcgaac ggcgtgaaac gccttcatcg tggcgacgat
      181 gagcccccag aggacgagga tctcgacaaa ggcccacatc ccctgacgcc agacgaagaa
      241 gatccacgtc cagagcgcgt tgagcacaag ctggtcgatg aagagagaga gcgggacgag
      301 ccccccgcgg aagcccctct cgcgccagac gagccacgcg gcgacgccca tcatcgcgta
      361 gagcgccgtc cacacggggc cgaagagcca cccgggaggc gcccacgaag gtcgcgccag
      421 ctccttatag aacgaggcgg attccaccga ggcaagcccc ccgacggccg ccgccgcgaa
      481 gacgattccg agccagaaca gcagtccgat acttttcgac atcattcctc cccctttcga
      541 ttcgccgtcc attcgctcgc cgattctccg cgaagctgcc ggaactatcc ggcgaatccc
      601 gcataagtgt acttcctctt ccccgtccgg tccagagcgc cttatcggga ggaggggcac
      661 tcctccccgg ctctttttcg cgcggcgagc gcgtcgaggt tgtgcgcgac ataagaggac
      721 gacgcaggcg ctcaacgcac gtcgtctcat cggcagtttc gcgtgcaaaa acaacctccg
      781 gcgtataaaa tagaggtatt cggcacacgc ataaaagaac ggctcgcagt agaatgtttt
      841 aaaagcggtt cctgcgcagg cttgggagag gttgcgtgca actcaggaca ggaggtgttt
      901 tcatggaggc cgcgacaccg tacgaaacca tactctatct cgcccgcaaa tacatctggt
      961 ggaagactcc tgaagaagcc gccgccatgc ctgagcgtgt tctggcccag gtgatgaaca
     1021 tcggggacta tgacgacgtg cttctgatga aatcccgctt cgaggacgat gcgctccgca
     1081 acgtgatcgc gcacgccgaa gcagggcagt tcaacgccag gtcatggcac tactggcatt
     1141 acatgctcgg gctcgcagag atcgaccacg tccctccgct cccgacaaga cgcttttcgt
     1201 ggagaggggc ggggaaaact acttcttgaa gaggtcggag tgggttccgg tgcgctccag
     1261 tgtcagaacg agcgattctt ccgtgatctt gtagatgaga agccagtcgg gacgtatgtg
     1321 gcattctcta tactccgcgt agtccccgga caatgtatga tccctctctt gcctcggaag
     1381 agtctccccg cttgccaatg ctcgtatgac atcggcgaga agggagatat cgcatcccct
     1441 tctttcgacc aacgcgaaat ccctcttgaa ccgcgaggtt ctctggattt cgtatcggag
     1501 cgtcatgacc tcagttcggt cagaaggtct tcgacggtcg aaaatcgcaa cgcgtcgggg
     1561 tctttcagca tccgctcgcc ttcttccagc gcggctacgg tctcttcgtt ggggcgctcc
     1621 tcctcgaggg cgtcgatgta gccgagcacc cgcacggcat tccgctccga gagtttgcgg
     1681 aaacggcgat acagttcttt tttggcggaa atttcaagtg tagtccgagg cattggtatc
     1741 cccccttcgc gtaggagtat tgtacagcga attccggcac gcttcaacgc cggggagacc
     1801 tccgcgttac tgcgtcagcc ggcggtactc ctgcctgctt tcccgctata aaaggcaatc
     1861 gcaatgaccg aattttatgc ggtgatagac agagacgagg acggtcaatt tttcgcctac
     1921 gtcccggagc ttccgggctg ccatacgtgc ggcgagacca tggaggagct tctggagaat
     1981 cttgaagagg cgatcgcgct gtacctcgag gttgtggagt atccccgcga gtttcccagc
     2041 atccggaaag tagccgtcgg atgagcccga agctcccgag aatgaccggt gacgaggcga
     2101 tatcgttcct tcgcttttca actgccgact
//
LOCUS       DAOTIL010000476         2129 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminobacteriaceae bacterium isolate AMR_MDS_3026
            NODE_61617_length_2129_cov_2.609450, whole genome shotgun sequence.
ACCESSION   DAOTIL010000476 DAOTIL010000000
VERSION     DAOTIL010000476.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868050
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminobacteriaceae bacterium (wastewater metagenome)
  ORGANISM  Aminobacteriaceae bacterium
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae.
REFERENCE   1  (bases 1 to 2129)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2129)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:19:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,224
            CDSs (total)                      :: 3,198
            Genes (coding)                    :: 3,184
            CDSs (with protein)               :: 3,184
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2129
                     /organism="Aminobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_61617_length_2129_cov_2.609450"
                     /isolate="AMR_MDS_3026"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:3034609"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..236
                     /locus_tag="P5201_09715"
     CDS             <1..236
                     /locus_tag="P5201_09715"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRV98858.1"
                     /translation="GSAVSPEIRGSGGKASLSWTPEKGKTPESFALDLVMFYTLEFYK
                     RGTMELWLDAGAPVRYENMSVSFPVGVYFCPVR"
     gene            269..>2129
                     /locus_tag="P5201_09720"
     CDS             269..>2129
                     /locus_tag="P5201_09720"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NHL repeat-containing protein"
                     /protein_id="HRV98859.1"
                     /translation="MKRACELQCDGIVFGGKKGAAHEDAFFCLFRRAWILIVCLLTLF
                     AFFPADAGAQEADPPALTDAPEGMLFRWVPPYYMTIPSGWKPMVERREGVGFFTGAHP
                     EAFEGASSEDVSMAALMVMKQTPPKGGGYKSFYEDIEKEASKGKVKNFVSREEETTIG
                     GQPAVFYSFSGEMEVGSGSRKMEGNMVLSVQPDAEGKHTLVALMGTSSSLEQLRDEIK
                     SILASAREARAPLRPLASFPYGSTQESFRHIYGPAVAADGAIAVAETIGEGKVRIFDS
                     KGAQLAEWGSRGKGEDGTFNYPQAMTFGPDGSLYVVDGGYTVDPHVQRFSRTGEFLGK
                     IRADKKSRGDDGIYTPSFLAVTDSGKIVIAGLTEITGGVPRVVVFSPEGDVLSKWEPG
                     EIGGIVALPGDRLLVTKPHPENDRNPLFTVLDLEGKTLKEWPFFGSDLPSTPGDEKTY
                     FRPKFITADAQGRVYAYDDSEDAVWMYDADGKFMYALPVRPPLGIVGGMAASPRGDLI
                     FHDRPSGYGPGEPSIHLFENAFPASAPALPAPEAVPETTEKPPVASEAAEESTPLEVE
                     LARLKKALALREEAERLENEGDLAGAAVKYKEKPRGKDQAEGVVVGGVHVARHV"
BASE COUNT          424 a          599 c          723 g          383 t
ORIGIN      
        1 cgggttccgc cgtcagcccg gagatccgag gcagcggagg aaaggcatcg ctctcatgga
       61 cgcccgagaa ggggaagacg ccggagtcct tcgccctcga cctcgtgatg ttctacacgc
      121 tggagttcta caaacggggg acgatggagc tttggctcga tgccggggcg cccgtgcgct
      181 acgagaatat gtccgtgtcg ttcccggtag gggtctactt ctgcccggtc cgctaatgag
      241 catgatgatg aaagaggagg gagtagtcat gaaaagagcc tgcgaactgc agtgcgatgg
      301 tattgtcttc ggcgggaaga agggagcggc gcatgaagac gccttcttct gcctgtttcg
      361 gcgggcgtgg attctgattg tctgtctgct gacgctgttt gcgttcttcc ctgcggacgc
      421 gggggcgcag gaggccgatc cgccggccct gacggacgcg cccgaaggga tgctcttccg
      481 ctgggtcccc ccctattaca tgacgattcc ttccggctgg aaaccgatgg tggagcgcag
      541 ggagggggtc ggattcttca ccggcgcgca tccggaggcg ttcgaaggcg cgtcatcgga
      601 ggacgtctcc atggcggccc ttatggtgat gaaacagacg ccccctaaag ggggagggta
      661 taagagcttt tacgaggaca tcgaaaaaga ggcgtcaaag gggaaggtga agaacttcgt
      721 ctcgcgggag gaggaaacga cgatcggggg gcaacccgcc gtattctact ctttctccgg
      781 agagatggag gtagggagcg gttccaggaa gatggaagga aacatggtcc tgagcgtcca
      841 gcccgacgcc gaggggaagc atacgctcgt tgcgctgatg gggacctctt cctctctgga
      901 gcaattacgg gacgagatca agagtatcct ggcgtcggcc agggaggcga gagcgccgct
      961 tcgccccctc gcctctttcc cgtacggttc cacccaggag agtttccggc atatctacgg
     1021 gcccgccgtg gccgcggacg gcgcgatcgc cgtcgctgaa accattggag agggaaaggt
     1081 gcgcatcttc gactcgaaag gagcgcagct cgccgaatgg gggagcaggg ggaagggaga
     1141 agacggaacc ttcaactatc cgcaggccat gactttcggc cccgacggtt cgctctacgt
     1201 cgtcgacggc ggctacactg tcgaccccca tgtgcagcgg ttttcccgca caggagagtt
     1261 tctgggcaaa atacgcgccg acaaaaaatc cagaggcgac gacggaatat acactccttc
     1321 attcctggcg gtcacggatt cgggaaagat tgtgatcgca ggcctcaccg agatcaccgg
     1381 cggcgtgccg cgcgtcgttg ttttttctcc cgaaggggac gtgctctcga agtgggaacc
     1441 gggagagatc ggaggaatcg tcgcgcttcc gggagatcgt ctgcttgtga cgaagccgca
     1501 tcctgagaac gacaggaacc ccctcttcac ggtgcttgac cttgagggga aaacgctgaa
     1561 ggagtggccg ttcttcggaa gcgaccttcc ttcgacgcca ggcgacgaga aaacctactt
     1621 caggccgaag ttcataaccg ccgacgcgca gggcagagtg tacgcctacg acgattcgga
     1681 agacgcggtc tggatgtacg acgccgacgg aaagttcatg tacgcccttc ccgtaagacc
     1741 gccgctgggt atcgtcggcg ggatggcggc ttcgccgcgc ggcgacttga tcttccacga
     1801 ccgtccgagc ggctacggcc ccggcgagcc gtcgattcat ctgttcgaga acgcgtttcc
     1861 cgcgtcggcg cccgctcttc ctgcgcccga ggccgtgccg gaaaccacgg agaaacctcc
     1921 cgtcgcgtcg gaggcggcgg aggagagcac tccgcttgag gtcgagctgg ccaggctgaa
     1981 gaaagcgctc gcgcttcggg aagaagcgga acgtcttgaa aacgagggcg atcttgccgg
     2041 agcggcggtg aagtacaagg agaagccgcg cggcaaggac caggccgagg gtgtggtcgt
     2101 gggcggcgtc cacgtcgccc gacatgttg
//
LOCUS       DAOTIL010000477         2125 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminobacteriaceae bacterium isolate AMR_MDS_3026
            NODE_61751_length_2125_cov_4.442029, whole genome shotgun sequence.
ACCESSION   DAOTIL010000477 DAOTIL010000000
VERSION     DAOTIL010000477.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868050
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminobacteriaceae bacterium (wastewater metagenome)
  ORGANISM  Aminobacteriaceae bacterium
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae.
REFERENCE   1  (bases 1 to 2125)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2125)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:19:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,224
            CDSs (total)                      :: 3,198
            Genes (coding)                    :: 3,184
            CDSs (with protein)               :: 3,184
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2125
                     /organism="Aminobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_61751_length_2125_cov_4.442029"
                     /isolate="AMR_MDS_3026"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:3034609"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            1..1303
                     /gene="ffh"
                     /locus_tag="P5201_09725"
                     /pseudo
     CDS             1..1303
                     /gene="ffh"
                     /locus_tag="P5201_09725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013047953.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_component: GO:0048501 - signal recognition particle,
                     plasma membrane targeting [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005048 - signal sequence binding
                     [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle protein"
     gene            1383..1658
                     /gene="rpsP"
                     /locus_tag="P5201_09730"
     CDS             1383..1658
                     /gene="rpsP"
                     /locus_tag="P5201_09730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013047954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S16"
                     /protein_id="HRV98860.1"
                     /translation="MAVRIRLARHGRKKAPFYRLVVADSRSPRDGRFIELLGTYDPMK
                     DPSSINVDEERAMHWLKVGATASDTARSILRKSGVWEKFEAGKKQAE"
     gene            1661..1909
                     /locus_tag="P5201_09735"
     CDS             1661..1909
                     /locus_tag="P5201_09735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006582993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KH domain-containing protein"
                     /protein_id="HRV98861.1"
                     /translation="MPDYRELVEFIVRRLVTQPDEVQISEQTDERGVTAITIRVAPED
                     VGRVIGKRGATINAIRLVAKASAVKVNDRVDVDIVEDE"
     gene            1902..>2125
                     /locus_tag="P5201_09740"
     CDS             1902..>2125
                     /locus_tag="P5201_09740"
                     /inference="COORDINATES: protein motif:HMM:NF013907.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA processing protein RimM"
                     /protein_id="HRV98862.1"
                     /translation="MNRVRIGRIVGTHGLRGALKIVPLTDYPERFHSMEKMSVYSAEG
                     QIITTLTLLSSRYSDHKKLLVIQTEELRRI"
BASE COUNT          535 a          519 c          639 g          432 t
ORIGIN      
        1 aaagggcgcg gcaaactcac cgaggcagat gtggaaactg cgttgaggga tgtgcggcga
       61 gggcttttag aagccgatgt cgattataaa gtggtaaagt ctcttctgga ggccataaaa
      121 actcgcgcca taggaagaga ttctcgaatc catcaccccg gggcaacatg tcgctgcaat
      181 cgtctatgaa gaacttgcag agttgatggg aaaagaacct gtaccccttg ttatttctcc
      241 aaagccgccg actgtctacc tcatggtcgg tttgcagggc ggaggaaaaa caacgagcac
      301 ggtgaagata gcgcgaaagc tttccggggc gcataaaccc ctcgtggttg cttgcgacct
      361 tcgtcgcccc gccgctgtcg agcagcttcg ggtactggct gcacaatcga aaatttcatt
      421 ttttggtcct gaaaatggag aaaaaaatgt cctcaacgtc gtaaaagctg ctctgagcta
      481 tgcggatgac catctgaacg acgttgtgct tcttgatacg gccggacgtc tccatgccga
      541 cgaggattta atggatgagc ttgtcgccat gaagaagtcc gctcctccta ctgagatact
      601 ccttgtcgtc gacgccatga ccggccagga ggcggtaaag gttgcctctt catttcatga
      661 gaaactccaa ctgacaggag tcgttctctc aaagctcgac ggagacgccc gcggaggcgc
      721 cgccctcgca attcgcaagg tgaccggagt gccggtcaag ctggccggtg ttggagaggc
      781 aacggacgct ctcgaagtct tcgatgcgaa gaggatggcc cagagaatac tcggaatggg
      841 cgatgtcatc ggcctggcgg aaaaaattca ggagatggcc gattccggcg gggccgagcg
      901 catggccgag acgctgaaga agaaatttac acttgaggag cttcttgcgc agttcgagca
      961 gcttgagaaa atggggcccc tgaacaaagt ccttgagatg atccccggat tcagcaagat
     1021 gaaaggcgtg tcccaggacg aagtcgattc ggggcggttg aaaaaatcga aggctatcat
     1081 tcagtcgatg actcgcgccg aacggaggga tccttcgatc atcaagggca gccgcagacg
     1141 tcggattgcg atgggctcgg gaacatcggt ccagatggta aatcagcttc tggcccagta
     1201 cgagcagatg cggaagctat ggaagcagtt cggaaaacag gggaagggct tcaaactacc
     1261 gaaaggcctc cttggaggcg gcggcggttt tcgttttaaa tagaacgttc ccggtgacgg
     1321 agcactccgg ggaagcaatg tattcgatgt gttccggaac aatatcagga ggtgtatggt
     1381 aaatggcagt acgtattcgc cttgcccgtc acggacggaa aaaggctcct ttttatcggt
     1441 tggtggtggc ggattctcgt tcacccagag acggtcgctt catcgaactt cttggaacct
     1501 acgacccaat gaaagatccc tcttcaatca atgtggacga agagcgggcg atgcactggc
     1561 tgaaagtcgg cgcgacggct tccgacaccg ccaggagcat tttgagaaag tccggggtct
     1621 gggaaaagtt cgaagcgggc aagaaacagg cggagtagtc atgcccgact accgggagct
     1681 tgttgaattc atcgtccgac gcctcgtgac acagcctgac gaggttcaga tcagtgagca
     1741 gacggacgaa aggggcgtca cggcgatcac cattagagtc gctccggagg atgtcggaag
     1801 ggtcatcggg aaacgcggcg cgacgatcaa cgcgattcgc ctggtagcaa aagcttccgc
     1861 agtgaaagtg aacgaccggg ttgacgtcga tatcgtcgag gatgaataga gtacgcatcg
     1921 gacgcattgt cggaactcac gggctgagag gggcgctcaa gatagtaccc ctgaccgatt
     1981 atccggagag atttcactca atggaaaaaa tgtcggtgta tagcgcggag ggacagatta
     2041 tcaccacgct cacgcttctg tccagccgat actccgacca caagaaactc cttgtgattc
     2101 agaccgagga gctgcgtcga ataga
//
LOCUS       DAOTIL010000478         2124 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminobacteriaceae bacterium isolate AMR_MDS_3026
            NODE_61803_length_2124_cov_3.108748, whole genome shotgun sequence.
ACCESSION   DAOTIL010000478 DAOTIL010000000
VERSION     DAOTIL010000478.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868050
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminobacteriaceae bacterium (wastewater metagenome)
  ORGANISM  Aminobacteriaceae bacterium
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae.
REFERENCE   1  (bases 1 to 2124)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2124)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:19:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,224
            CDSs (total)                      :: 3,198
            Genes (coding)                    :: 3,184
            CDSs (with protein)               :: 3,184
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2124
                     /organism="Aminobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_61803_length_2124_cov_3.108748"
                     /isolate="AMR_MDS_3026"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:3034609"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(172..1152)
                     /locus_tag="P5201_09745"
     CDS             complement(172..1152)
                     /locus_tag="P5201_09745"
                     /inference="COORDINATES: protein motif:HMM:NF024676.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="WYL domain-containing protein"
                     /protein_id="HRV98863.1"
                     /translation="MPRDVSSVRLSRLNRLMMLFYRYPRVSREDILEGVGYASGRTFE
                     RDAEFLRDEYGVEISYSRETRLYTLCSRGTFILNCTMGEKEVLALSAGLCMAGHFLPH
                     LREEAAELWSKLKALLPEELAQQGDELGRSAVVALPVSTIDPVVFETILDACRSARTL
                     EIDYTSPYGDGASRAHVVYPWGVFFQAHAWYLWAGNVKYPDTGTYRISRIGSVRPVPG
                     IPRIDPPEGEDLRSYASSAWYACAGKACTPVGIKVLPPLARVVAETVWHPSQRVEECE
                     DGAIILRASVPDIGEVVRWMLAGAPYALPLEPPELRERLLQTMERLKEAL"
     gene            complement(1326..>2124)
                     /gene="yedE"
                     /locus_tag="P5201_09750"
     CDS             complement(1326..>2124)
                     /gene="yedE"
                     /locus_tag="P5201_09750"
                     /inference="COORDINATES: protein motif:HMM:TIGR04112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="YedE family putative selenium transporter"
                     /protein_id="HRV98864.1"
                     /translation="SSPLLRLLLGFFTMVGAKIFLGCTWRAYARLAEGDWNALAGIGG
                     VIAGVGTGVYFLRQGFALGRAGKVSVRSGGLLPLTSCVLLAILLFRPVFFPASGPDMS
                     LVSVALSVVVGLLVGWMAQKSRYCTVGAIRNLIMARDAHLLYGIIAFLIASFTMNILL
                     DQFMPGFDNQPLEHTNHLWNFLGMLLLGLAATFAGGCPGRQFVLSGQGDGDAAVFLLG
                     MLAGGLFSCLVPLTGLSEGVTVYGMAAVVLGIIFCLAVGFLCRIRME"
BASE COUNT          462 a          614 c          680 g          368 t
ORIGIN      
        1 gtcttcattt tcgcattcct ccttgtattt tgaggggcca agcccctctg ttcagaagat
       61 accagaagga agcgacaagt tacgtcgttt caaaaacctt ttttaaaaat tcgacgtgcg
      121 gacgggaacg tgtcggggta ttcggcatcc cggcgccgag attgccggcg cttacagcgc
      181 ctctttcagg cgctccatcg tctgcagcag gcgttcgcgg agttccggcg gctccagagg
      241 gagggcgtag ggggcgccgg cgagcatcca gcggacaacc tcgccgatgt ccgggacaga
      301 ggcgcggagg atgatggcgc cgtcctcgca ctcctccacc cgttgggacg ggtgccagac
      361 ggtttccgcg acgactcgcg caaggggcgg cagcaccttg atcccgaccg gcgtacaggc
      421 ctttccggcg caggcgtacc atgccgagga ggcgtaggag cgcaggtcct ctccctcggg
      481 cgggtcgatc cgcgggatac cggggacggg gcggactgag ccgatgcggg agatgcggta
      541 ggttccggtg tccgggtatt ttacgttgcc cgcccagagg taccaggcat gcgcctggaa
      601 gaagacgccc caggggtaga cgacgtgggc gcgggaagcg ccgtcaccgt agggggaggt
      661 gtagtctatt tcgagggtgc gcgccgagcg gcaggcgtcg aggatggttt cgaagacgac
      721 ggggtcgatc gtcgagactg ggagcgcgac cacggcggag cgtcccagtt cgtccccctg
      781 ctgggccagc tcctcgggga ggagggcctt cagcttgctc cacagctccg cggcctcctc
      841 gcggaggtgc ggaaggaagt gccccgccat gcacaagcct gcggagaggg cgagcacttc
      901 tttctccccc atcgtgcagt taagaatgaa ggttccgcgg ctgcacaggg tgtagaggcg
      961 ggtctccctc gagtaggaga tctcgacgcc gtactcgtcg cggagaaact ccgcgtcgcg
     1021 ctcgaaggtc cgtcccgaag cgtacccgac gccttcgagg atatcctcgc gggagacgcg
     1081 gggatatcgg tagaagagca tcatgagacg gttgaggcgc gagagacgga cggagcttac
     1141 gtcgcgcggc attgtggatg acctccttgt gtggaacggt gtgagaatac atctcagagc
     1201 aggagtaggc aaatcggaat gtaccacgca ccctgtgaca gatagaggat caattcaccg
     1261 aaagagagct catatcaaag gatgcagcgt catcgttttc tttttcagcc cggtcgtctt
     1321 cctcttcact ccattctgat acgacagagg aagcccacgg cgaggcaaaa gatgatgccc
     1381 agcaccacag ccgccatgcc gtacaccgtc actccctccg aaagtccggt aagcgggacg
     1441 aggcacgaga agagcccgcc cgcgagcatc ccgagcagga agaccgccgc atcgccgtcg
     1501 ccttgccccg aaaggacgaa ctggcgtccg gggcatcctc cggcgaatgt ggcggccagc
     1561 cccagaagca gcatgcccag gaaattccaa aggtgattgg tgtgttccag aggctggttg
     1621 tcgaatccgg gcatgaactg gtcgaggaga atattcattg tgaacgaggc aatcaggaag
     1681 gctatgattc cgtaaaggag gtgggcatcc ctggccatta tcaggttacg gatggctcct
     1741 acagtgcagt agcggctctt ttgcgccatc caccctacca gtaaacctac gacgactgag
     1801 agcgccaccg aaaccaaaga catgtccggt ccggaggcgg ggaagaaaac cggtcgaaag
     1861 aggagaatcg ccaacagaac gcacgatgta agaggaagga gacctcctga tcttacggag
     1921 acctttccag ctctcccgag agcaaagccc tgcctcagaa agtagacgcc tgttccaacc
     1981 ccggcaatca cccctccgat gccggccagc gcattccagt ctccttcggc gagacgggca
     2041 tacgcccgcc aggtacagcc aagaaatatc ttggcgccaa ccattgtaaa aaatccgagc
     2101 agaagtcgaa gcaagggaga tgag
//
LOCUS       DAOTIL010000479         2120 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminobacteriaceae bacterium isolate AMR_MDS_3026
            NODE_61938_length_2120_cov_3.328329, whole genome shotgun sequence.
ACCESSION   DAOTIL010000479 DAOTIL010000000
VERSION     DAOTIL010000479.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868050
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminobacteriaceae bacterium (wastewater metagenome)
  ORGANISM  Aminobacteriaceae bacterium
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae.
REFERENCE   1  (bases 1 to 2120)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2120)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:19:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,224
            CDSs (total)                      :: 3,198
            Genes (coding)                    :: 3,184
            CDSs (with protein)               :: 3,184
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2120
                     /organism="Aminobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_61938_length_2120_cov_3.328329"
                     /isolate="AMR_MDS_3026"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:3034609"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..664
                     /locus_tag="P5201_09755"
     CDS             <1..664
                     /locus_tag="P5201_09755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013048999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="translational GTPase TypA"
                     /protein_id="HRV98865.1"
                     /translation="PEVITEERDGKTFEPMEDLVVDIPEEYQGVVFEKLSRRKARVQE
                     IQNLRTGLMRIFFAVPTRGLIGYRGEFLTDTRGLGIMSSRFSGYAPWVGEITPRNRGA
                     LVSLDTGEATSYQLENLQERGVLFISPMDKIYSGMIVGENARPGDMPCNPTKRKQATN
                     HRSSTKDFTIKLDVPRKMTLEKALEWISEDELVEVTPKSIRLRKSILDDNDRRKARRI
                     SA"
     gene            complement(707..1840)
                     /locus_tag="P5201_09760"
     CDS             complement(707..1840)
                     /locus_tag="P5201_09760"
                     /inference="COORDINATES: protein motif:HMM:NF017221.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HRV98866.1"
                     /translation="MPLDELSRKLKEIQDKMSEALHYSIDSISSLREEERERLFEVRT
                     RKEDLIRELNEVIVAADKAESEYRRSREELLVASRSGNERREKDAYERAMHLMKIRGA
                     FEEREKLLARQRDDLAREERRIEKLLVRSEEMGNRFRVALNLLSMNLEGESPASSDES
                     GLLAGMVLAERESVSLSRDLHDGPIQKFSSIGLMIDLSKEYLDRGDFVRAGEELGRTR
                     AHLADALLEARSFLFQLNPSGLKEGLHTPLSRLASQVRSFSGCDVRFSIEGRADVLSL
                     QVRTAVFKIVQQAVMNAVRGGKAKNVKIMAGIGGDALRVKVLDDGIGFDVAKERELAE
                     ERGTWGLVNMEERARLIGGSVSITSEPGRGSTVFLEVPLTKKV"
BASE COUNT          426 a          695 c          576 g          423 t
ORIGIN      
        1 accggaggtc atcaccgagg agcgcgacgg aaaaaccttc gagccgatgg aggatcttgt
       61 cgtggacatc cccgaggagt accagggcgt cgtcttcgag aagctgtcgc gacgcaaggc
      121 gcgcgtgcag gagatccaga atctccgtac gggactgatg cgcatcttct tcgccgtccc
      181 cacccgcggc ctcatcggct accgggggga attcctgacc gacacgagag ggcttgggat
      241 catgtcgtcc cggttctccg ggtacgcccc gtgggtaggc gagatcacac cgcgcaacag
      301 gggcgcgctg gtcagcctgg acaccggcga ggccacaagc taccagcttg aaaatctcca
      361 ggagcggggc gtcctcttca tctcgccgat ggacaaaatc tactcgggaa tgatcgtggg
      421 ggaaaacgcg cgcccgggcg acatgccctg caacccgacg aagcgcaagc aggcgaccaa
      481 ccacagatcc tccacgaagg actttactat aaagctcgac gtcccgagga aaatgaccct
      541 cgaaaaagcc cttgaatgga tctccgagga cgaactcgtc gaggtgacgc ccaagtcgat
      601 ccgtcttcgc aaatccatcc tggacgacaa cgatcgaaga aaggctcgac gcatcagcgc
      661 ataaaaagac aagcgccgct cctccaacgg ggagcggcgc ttgtctttac actttcttcg
      721 tcagcggcac ctcgagaaaa acggtcgacc ctctgcccgg ttcgctggta atagagacgc
      781 tcccaccgat caggcgagcg cgctcctcca tgttcacaag cccccacgtg ccacgttcct
      841 cggccagttc gcgctccttc gccacatcaa aaccgatgcc gtcgtcaagc acctttacac
      901 gaagggcatc cccgccgata ccggccatta tcttcacatt cttcgctttt ccgccccgga
      961 cggcgttcat caccgcttgc tggacgatct tgaagaccgc tgtacggacc tggagcgaca
     1021 gaacatccgc acgtccttca atcgaaaaac gaacgtcgca ccctgagaag gatcgtacct
     1081 gagaagcaag gcggctcaga ggggtgtgca ggccctcttt cagccccgag gggttgagct
     1141 ggaagagaaa agaacgcgct tcgagaagag cgtccgccag gtgcgccctc gtccgtccca
     1201 gctcctcccc cgcacggaca aaatctcccc tgtcgagata ctctttggag aggtcgatca
     1261 tcagcccgat gctggagaac ttctgaatgg ggccgtcgtg gagatcccgc gacagcgaga
     1321 cgctctcccg ctccgcaagc accatacccg caagaaggcc gctctcgtcg gaggaagccg
     1381 ggctctcccc ctccaggttc atgctcagca ggttcagcgc aacccggaag cgattcccca
     1441 tctcctcgct ccgcacgagc agcttctcga tgcggcgctc ctcccgggcg agatcgtccc
     1501 tctggcgggc gagcagcttc tctcgctcct caaaagctcc ccgtatcttc atcaggtgca
     1561 ttgcgcgctc gtaggcgtct ttctcgcgcc tttcattccc ggaacggctg gctacgagaa
     1621 gctcctcccg tgaccggcga tattcgctct ccgccttgtc ggcggcgacg atcacctcgt
     1681 tgagctccct gataagatcc tccttgcgcg tgcggacctc gaagagccgc tcccgttcct
     1741 cctcgcggag cgacgagatg ctgtcgatac tatagtgcag cgcctcgctc atcttgtcct
     1801 gtatttcttt cagcttccgt gacagttcgt ccaagggcaa gcgcggcgcc tccttttttc
     1861 tctttgaacc cggtacgcgc atcgtttctc atatatcgct tattgtaccg ttgaaacgga
     1921 ggtctggcaa ttttcgccgg aacggataca atgaagtgac tttctcatcg aaggagatgc
     1981 atttcatgat cctcgtgctc attcttggct gggcggtgct ctacgccgac cgtacggccc
     2041 tctaccccct gctctcggtc atcgcggaga cgctggggat ttcgtccgca caggcggggt
     2101 ccattaccag tgcgtacttt
//
LOCUS       DAOTIL010000480         2118 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminobacteriaceae bacterium isolate AMR_MDS_3026
            NODE_62005_length_2118_cov_3.841493, whole genome shotgun sequence.
ACCESSION   DAOTIL010000480 DAOTIL010000000
VERSION     DAOTIL010000480.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868050
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminobacteriaceae bacterium (wastewater metagenome)
  ORGANISM  Aminobacteriaceae bacterium
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae.
REFERENCE   1  (bases 1 to 2118)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2118)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:19:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,224
            CDSs (total)                      :: 3,198
            Genes (coding)                    :: 3,184
            CDSs (with protein)               :: 3,184
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2118
                     /organism="Aminobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_62005_length_2118_cov_3.841493"
                     /isolate="AMR_MDS_3026"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:3034609"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..740
                     /locus_tag="P5201_09765"
     CDS             <1..740
                     /locus_tag="P5201_09765"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: protein motif:HMM:NF024703.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-dependent DNA helicase"
                     /protein_id="HRV98867.1"
                     /translation="SAEKMIFISATLALGGKTDFWERETGIHPDRLFISGSPFDLEQQ
                     MEILVVDTGLDVMNPRYDDTVCRIVEKLVEANGGSTLVLLASHRLLGKVGRALSSKPR
                     GYRVLVQGEGPRSELLAAFRSDPSSVLVGSASFREGVDVPGDGLTQVIIDRIPFPHPG
                     DPLVQARNALEGAGAFVRVTLPEAKMLLKQAAGRLIRSRSDRGRVAILDGRVLGRPEW
                     KIPSALPKVKYRRLVVARSAVAPSGGV"
     gene            813..947
                     /gene="rpmH"
                     /locus_tag="P5201_09770"
     CDS             813..947
                     /gene="rpmH"
                     /locus_tag="P5201_09770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012082253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L34"
                     /protein_id="HRV98868.1"
                     /translation="MKQTFQPHNRPRKRKMGFLARSSSPTGRRILRNRRRKGRARLAV
                     "
     gene            951..1352
                     /gene="rnpA"
                     /locus_tag="P5201_09775"
     CDS             951..1352
                     /gene="rnpA"
                     /locus_tag="P5201_09775"
                     /EC_number="3.1.26.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013048093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004526 - ribonuclease P activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease P protein component"
                     /protein_id="HRV98869.1"
                     /translation="MRFFFPRSSRLRRGWEFDSVFRTGDRLRGELVRLLFLKAEDGVT
                     RIGLAVGKRQGKSHDRNRGRRVLKEGFRRILPWIRPGVWIVAGLSGTGMKAGSTDVYE
                     ELARLLKGGGFLEPEWPGANWENPSEGAKEK"
     gene            1349..1621
                     /gene="yidD"
                     /locus_tag="P5201_09780"
     CDS             1349..1621
                     /gene="yidD"
                     /locus_tag="P5201_09780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004933881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertion efficiency factor
                     YidD"
                     /protein_id="HRV98870.1"
                     /translation="MTPAAWVGITLVRGYQLFVSPLLGHNCRFYPTCSQYAIDAISVH
                     GFLKGSFLAVKRLCKCAPWHPGGYDPVPPAARREEQGAYSYKDGDL"
     gene            1657..>2118
                     /gene="yidC"
                     /locus_tag="P5201_09785"
     CDS             1657..>2118
                     /gene="yidC"
                     /locus_tag="P5201_09785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013048095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0051205 - protein insertion into membrane
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertase YidC"
                     /protein_id="HRV98871.1"
                     /translation="MRLMELVHGFTGSWGLAIIVLTLIVRLLLHPLTKKQMTSMQKMQ
                     KLQPRIKMLQEKFKDDKESLNREMMALYKENKVNPAAGCLPLLVQLPVFILLYQVLTN
                     YDFSGVSFLWIRLDGSVLTTLGEALKLTVEKDQLGIMTVLYGLMANPYALLN"
BASE COUNT          432 a          558 c          672 g          456 t
ORIGIN      
        1 cctccgccga gaagatgatc ttcatctccg caacgcttgc tctgggggga aagaccgact
       61 tttgggaacg ggagacggga attcacccgg accgtctttt tatcagcggt tctcccttcg
      121 acctcgaaca gcagatggag atcctcgttg tcgataccgg actcgacgtg atgaaccccc
      181 gctacgacga tacggtgtgc cgcatcgttg aaaagctggt ggaggcgaac ggggggagca
      241 cgctggtgct cctggcgtcg cacaggctgc tcggaaaagt cgggcgcgct ctgagctcga
      301 aacccagggg gtaccgcgtc cttgttcagg gggaaggtcc gcgcagcgag cttcttgcgg
      361 cattccggag cgatccgtcc tccgtgctcg tggggagcgc ctctttccgg gagggagtgg
      421 atgttcccgg agacgggttg acccaggtca tcatcgaccg gattcccttc cctcatccgg
      481 gggatcccct ggtgcaggcg aggaatgcgc tcgaaggggc gggagcgttt gttcgcgtca
      541 cgcttccgga ggcaaagatg cttttgaaac aggccgccgg aaggctgatc cgaagcagaa
      601 gcgaccgggg ccgcgtcgca attctcgacg gccgggtgct cggccgccct gaatggaaga
      661 tcccgtctgc gctgccgaaa gtgaaatatc gtcgtcttgt agttgcgcgt tccgctgttg
      721 ctccaagcgg gggtgtgtga tatagtatca aggctgaaaa aaagccgttg atcagcggct
      781 ggttcttcta ttgccgtcag gaggtgtcat tggtgaaaca gacgttccaa ccccacaaca
      841 gaccacgaaa aagaaagatg ggtttcctcg cccggtcgag ttcgcccaca ggccgtcgta
      901 ttctccggaa tcgcaggaga aagggccgcg cccggctggc cgtttaatcc ttgagatttt
      961 tttttcctcg gtcttcacgc cttcgaagag ggtgggagtt cgatagtgtc ttccgcaccg
     1021 gtgatcggct gagaggcgaa ctggtgcggt tgctgtttct gaaggcggag gatggcgtta
     1081 cacgcatcgg cctggcggtg ggcaaacgac aggggaagtc ccacgaccgg aataggggac
     1141 ggagagtatt aaaagaaggc ttccgcagaa ttcttccctg gatccggccg ggagtatgga
     1201 tagtcgcggg actcagcggc acgggaatga aagcgggctc gacggatgtc tacgaagagc
     1261 tggcccgact tctcaaaggg ggagggtttc tggagccgga atggccggga gcgaactggg
     1321 aaaatccgtc ggagggcgca aaagaaaaat gacgcccgct gcatgggtcg gaattacact
     1381 cgttcggggg taccagcttt ttgtgtcccc gctcctcggg cataactgca gattctatcc
     1441 gacgtgctcg cagtatgcga ttgacgcgat ttccgttcat gggttcctga aaggtagctt
     1501 tttagcggtg aaacggcttt gcaagtgcgc cccgtggcac cccggagggt atgatcccgt
     1561 accgccggcg gctcgaagag aggagcaggg ggcttattcg tacaaggatg gtgatctata
     1621 gtgggttcta tttggaagat gggcggggac cttatgatga ggctgatgga gcttgttcac
     1681 ggcttcacag ggtcctgggg gttggcaatc atcgtactca cgctgattgt ccgtttgctt
     1741 ctgcaccccc ttacaaagaa gcagatgacg agcatgcaga agatgcagaa actccagccc
     1801 cgtatcaaga tgctgcagga gaagttcaag gacgacaagg agtcgcttaa ccgcgagatg
     1861 atggcgctct acaaggagaa caaggtaaac ccggccgccg gatgccttcc tcttcttgtc
     1921 cagcttcccg tcttcattct gctctaccag gtgctgacca actacgactt ttcaggcgtc
     1981 tctttcctct ggatacggct tgacggttcc gtgctcacta cgctcggcga ggcgctcaag
     2041 ctgactgtcg agaaggatca gctcggcatc atgaccgtgc tctacggcct tatggcgaac
     2101 ccgtatgcgt tactgaac
//
LOCUS       DAOTIL010000481         2118 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminobacteriaceae bacterium isolate AMR_MDS_3026
            NODE_62007_length_2118_cov_3.701890, whole genome shotgun sequence.
ACCESSION   DAOTIL010000481 DAOTIL010000000
VERSION     DAOTIL010000481.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868050
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminobacteriaceae bacterium (wastewater metagenome)
  ORGANISM  Aminobacteriaceae bacterium
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae.
REFERENCE   1  (bases 1 to 2118)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2118)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:19:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,224
            CDSs (total)                      :: 3,198
            Genes (coding)                    :: 3,184
            CDSs (with protein)               :: 3,184
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2118
                     /organism="Aminobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_62007_length_2118_cov_3.701890"
                     /isolate="AMR_MDS_3026"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:3034609"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..659
                     /locus_tag="P5201_09790"
     CDS             <1..659
                     /locus_tag="P5201_09790"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRV98872.1"
                     /translation="ACVEGEIPISRLDDAVRKVLEAKEALGLLQDEWEPHFAPRIAPD
                     GAATAREAALRSITVARNRSETLQTVRDGKKRPLLWPGERIAAGKVFCEFLFDVDLIE
                     VPERPEFHDISDILQRVEGVDRILVAEYDSKRNPDWLNLVRQIGEERTLLISLRTPYA
                     LLSLPSVSGAIVSYSDNTPSLQALADVLRGNAPAEGRLPVELPGFEIRTGGCQVTSGF
                     "
     gene            complement(968..1807)
                     /locus_tag="P5201_09795"
     CDS             complement(968..1807)
                     /locus_tag="P5201_09795"
                     /inference="COORDINATES: protein motif:HMM:NF012576.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000976 - transcription cis-regulatory
                     region binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HRV98873.1"
                     /translation="MEDVARFAGVNKATVSRALKGDPRISSVTSEKVWNAAKTLGYRI
                     DRTASGLSGGKTGLAAVVLESLRPWFSPLFFSGLNRVLGNAGMDILLKMPGFSDDALE
                     ASLVSRRVDCILLAGSGEGMAPSRFLLPQIPVVTAGFSIAETPSVFVSEQETLSRLRS
                     VSSGRPLRFVSGTSPLFPFLSRSITDSEAAPEEYFWIMDGCAPEDVGRTVRFGCVCTL
                     PEEPVRTGFFRIEWPAFEMGVAVGRLLMKASSGKDVFPTRLMPVPLVRDPAGEKILFG
                     KMN"
     gene            complement(1839..>2118)
                     /locus_tag="P5201_09800"
     CDS             complement(1839..>2118)
                     /locus_tag="P5201_09800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015556873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="nucleoside kinase"
                     /protein_id="HRV98874.1"
                     /translation="TLWPKVIRGSMQFVFPYQKHADVMFNSSLPYELAALRAYVDPIL
                     HTVPENSPVYGEAQRILSMIKFVPMIPTEIIPNNSIIREFIGGSCFDV"
BASE COUNT          526 a          567 c          563 g          462 t
ORIGIN      
        1 aagcctgcgt tgaaggggaa attccaatca gtcggctgga tgacgctgta cgaaaggttc
       61 tggaggcgaa agaagcgctt ggtcttcttc aagatgaatg ggaaccgcac tttgctccgc
      121 gcatcgctcc cgatggggct gctaccgcac gcgaagcggc gttgcgaagt atcactgttg
      181 ccaggaacag atctgaaacg ttgcaaaccg tacgagacgg gaaaaaacgc ccgctgctct
      241 ggcccgggga acggattgct gcgggaaagg ttttctgtga atttctcttc gatgtcgacc
      301 tgatcgaagt cccggaacgg ccggaatttc atgatatctc cgatatactg cagcgcgtag
      361 agggcgtcga caggattctg gtcgcggagt acgatagtaa gagaaacccg gactggctca
      421 accttgtccg gcagatcggt gaagaacgaa ctctcctcat ctcgcttcgg actccgtacg
      481 cacttttatc gttgccctcc gtgtcgggcg ctatcgtttc atatagcgac aacacccctt
      541 cgcttcaggc cctggccgat gtactccgag gaaatgcccc cgctgaagga agattgcccg
      601 tagagcttcc cggttttgaa atacgaacgg gaggatgcca ggtaacctcc ggtttttaag
      661 ctacaccgcc agggagagct ggtgttttac aggaaatgat gttattgccg cagagatggc
      721 gccgggttcc gaaaggaacc acgcgccatt ttcattttta tcccatacga gggatggggt
      781 gcagcatccg ctgcaaagag gagcgtcaca gaaccaattc ccgtttgtat ctttcatcca
      841 catgttgaaa acctcctcgt gtttttgtct gcttggcagc gactgacact gtttctttca
      901 cgaggatgtt ataccacgac ttttttcata agtcaacacc cgtttagctt ttctattcaa
      961 aaaaacctta gttcattttc ccgaagagta ttttttctcc cgcgggatcc cggacaagcg
     1021 gaacaggcat taggcgggtt gggaagacat ctttccccga cgacgctttc ataagaaggc
     1081 gcccgacggc aactcccatt tcaaaagccg gccattcgat acgaaagaaa cctgtacgta
     1141 ccggttcctc agggagagta cagacacatc cgaaacgaac cgtacgcccg acgtcctccg
     1201 gtgcgcaacc atccatgatc caaaagtatt cctccggggc tgcttccgaa tctgtgatac
     1261 tgcgtgagag aaaggggaaa agaggagacg ttcccgaaac aaaacgaagc ggacgccccg
     1321 aagagacgga gcgaagccgt gagagcgtct cttgctctga aacgaaaact gaaggtgttt
     1381 cagcaatgga aaaaccggcc gttacaacag gtatctgcgg tagcaaaaag cgggagggcg
     1441 ccatcccctc cccgcttcct gcaagaagaa tacagtcgac acggcgtgat acaagcgaag
     1501 cttccagcgc gtcatcgctg aaaccgggca tcttgagaag aatatccatt cccgcgttgc
     1561 cgagaacacg gttcaatcca gaaaagaaaa gcggactgaa ccagggtcta aggctttcaa
     1621 gcacaactgc cgcaaggccg gtcttgcctc cggaaagccc gcttgcggta cggtcaatac
     1681 gatacccgag agtcttcgca gcattccaca ctttttcact cgtgacggaa gagatgcgag
     1741 gatctccttt cagcgctctg ctgactgtgg ccttgttcac accggcgaac cgggccacat
     1801 cttccatcgt cgctcgcgtc acaataggga ggagggcgct acacgtcgaa acatgagccg
     1861 ccgatgaact ccctgataat cgagttgttc ggtatgatct ccgttggaat catgggaaca
     1921 aacttgatca tcgagagaat tcgctgcgct tcaccgtaga caggggagtt ttccgggacc
     1981 gtatgaagta tcggatcgac atacgccctc agagccgcca attcgtacgg tagagaggag
     2041 ttaaacatca cgtccgcatg tttctggtag gggaacacaa actgcatgct ccccctgatg
     2101 accttcggcc agagggtt
//
LOCUS       DAOTIL010000482         2118 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminobacteriaceae bacterium isolate AMR_MDS_3026
            NODE_62021_length_2118_cov_2.835191, whole genome shotgun sequence.
ACCESSION   DAOTIL010000482 DAOTIL010000000
VERSION     DAOTIL010000482.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868050
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminobacteriaceae bacterium (wastewater metagenome)
  ORGANISM  Aminobacteriaceae bacterium
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae.
REFERENCE   1  (bases 1 to 2118)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2118)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:19:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,224
            CDSs (total)                      :: 3,198
            Genes (coding)                    :: 3,184
            CDSs (with protein)               :: 3,184
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2118
                     /organism="Aminobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_62021_length_2118_cov_2.835191"
                     /isolate="AMR_MDS_3026"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:3034609"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1075)
                     /locus_tag="P5201_09805"
     CDS             complement(<1..1075)
                     /locus_tag="P5201_09805"
                     /inference="COORDINATES: protein motif:HMM:NF014587.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Ppx/GppA phosphatase family protein"
                     /protein_id="HRV98875.1"
                     /translation="MGPQLKSGHVVGLIDLGTNSVRLFVVRINENKSYTILNQHKIMV
                     RLGEGEFVKQRLKNEAMERTILVLGRFAEMARNLGAEEILAVATSATRDAENKDVFLE
                     RVRKEAGIDLKVISGLEEARLIYLGIASGMHLGDKTALFIDIGGGSTETIVGNQAEYF
                     HLDSLKLGAVRLASLFFLPGETDPVSPDRYALLQQYVRNASLRSVQRLREFPVDLVVG
                     SSGTLETLAEMAFRRGNKKQKGASAEKESSERTMTRRQLEELTKLLCSLSLEERKRLP
                     GMVPERADIIVAGASIIHTLMEDLELDKIRISTRSLRNGLLVDYLMRGKWGYLDAAFS
                     VREQSVLQLARSCGFHEEHSRHLG"
     gene            complement(1060..>2118)
                     /locus_tag="P5201_09810"
     CDS             complement(1060..>2118)
                     /locus_tag="P5201_09810"
                     /inference="COORDINATES: protein motif:HMM:NF014069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase family protein"
                     /protein_id="HRV98876.1"
                     /translation="EKAELHLWNRGRLSRESRKYLKNLPRTKKFLLAGKQFLLTHGSP
                     EDIEENIGPDTPDERLCEIARLASSDIVLTGHTHAPLSKKIGDTWFINPGTAGRPLGD
                     DLRACYALLHIQPGYFRVNHYLVDYDMAKTADAALFRGMPGDFASSFLPGRAVAGDIP
                     MEDEAERILETTIPVHADHSRHVASIALALFDRLEELHGLDREARFQLQIASTLHDIG
                     WLKGQKEHHKVAMEFILGLDGVIPDERERILIASVARYHRKADPDDSHPFFERLSPGD
                     RETVRKLAAILRIADGLDWTHSSVVKGVECLVGDGAVTVLCSTSGDAGAERARALEKG
                     ALFEKVFGKKLDIEWVRS"
BASE COUNT          335 a          648 c          705 g          430 t
ORIGIN      
        1 ctccgaggtg gcgcgagtgc tcctcgtgga agccgcacga gcgcgcgagc tgaaggacgc
       61 tctgttcgcg caccgagaat gcggcgtcga ggtaccccca ctttccgcgc atcaggtagt
      121 ccaccagcag accgttccgg aggcttcgcg tgctgatccg tatcttgtcc agctcaagat
      181 cctccatcag cgtgtggatg atggaggcgc cggcgacgat gatgtccgca cgttcaggaa
      241 ccatgccggg aaggcgtttc cgctcttcga gcgagaggga gcagaggagc tttgtcagtt
      301 cctcgagctg gcgccgggtc atggtccgtt cggagctttc cttctcggcg gaggcgcctt
      361 tttgtttctt gttcccgcgc cggaacgcca tttccgccag cgtttccagc gtgccggagc
      421 ttcccacgac gagatcgacc ggaaattcac ggagcctctg gacgcttcga agcgaggcgt
      481 tccggacgta ctgctgcagc agcgcgtagc ggtccggaga gactggatcg gtttccccgg
      541 ggaggaagaa gagcgacgcg aggcggactg cgccgagttt gagggagtcg aggtggaagt
      601 actccgcctg gttgccgacg atcgtctccg tgctgccgcc tccgatgtcg atgaagagcg
      661 ccgttttgtc gccgaggtgc atgccgctcg cgatgccgag gtagatcagg cgggcctcct
      721 ccagtccgga gatgaccttc aggtcgattc ccgcctcctt gcgcacgcgt tccaggaaga
      781 cgtccttgtt ctccgcgtct cgcgtggcgg aggtcgccac cgcaaggatc tcctccgcgc
      841 cgaggtttcg cgccatctcg gcgaatctgc cgagcacaag gatcgtccgc tccatggcct
      901 cgttcttgag gcgttgcttg acgaactccc cctcgccgag gcggaccatg atcttgtgct
      961 ggttcagtat cgtgtaggat ttgttctcgt tgatgcgcac gacgaaaagg cgcacggagt
     1021 tcgtcccgag gtcgatgagg ccgaccacgt gccccgactt cagctgcgga cccattcgat
     1081 atccagcttc tttccgaaga ccttctcgaa gagcgcgccc ttctccaggg cccgcgctct
     1141 ctccgccccg gcgtctcccg acgtcgagca gaggacggtc acggccccgt cgccgacgag
     1201 acactccacc cctttgacga ccgacgagtg ggtccagtcg agcccgtcgg cgattctgag
     1261 gatcgctgcg agctttcgta cggtctctct gtccccgggt gagagacgct cgaagaaggg
     1321 gtgactgtcg tcggggtcgg cttttctgtg gtagcgggcg acggaggcga tcaggatgcg
     1381 ctcccgctcg tcggggatga cgccgtcgag tccgaggatg aactccatgg cgaccttgtg
     1441 gtgctccttc tgccccttca gccagccgat gtcgtggagc gtcgaggcga tctggagctg
     1501 gaagcgcgcc tcccggtcga gtccgtgaag ctcttcgagg cgatcgaaga gggcgagggc
     1561 gatggacgcc acgtggcggc tgtggtcggc gtgcaccgga atggtcgtct ccaggatgcg
     1621 ttcggcctcg tcctccatcg ggatatcccc cgcgacggcc cgccccggca gaaaggagga
     1681 ggcgaagtct cccggcatcc cccggaagag cgccgcgtcg gccgtcttcg ccatatcgta
     1741 gtcgacgagg tagtggttca ccctgaagta gccgggttgg atgtgcagca gcgcgtagca
     1801 ggcacggagg tcgtctccaa gggggcgtcc ggccgttccc gggttgatga accacgtgtc
     1861 gcctatcttt ttcgagagag gggcgtgggt atgcccggtg agcacgatgt cggaggaagc
     1921 caggcgggcg atctcacaaa gacgctcatc gggagtgtcc gggccgatgt tctcctcgat
     1981 gtcctcgggg gagccgtgcg tgagcagaaa ttgcttgccc gcgagcagga atttcttcgt
     2041 ccggggaagg tttttgaggt acttgcggct ttcccttgaa aggcgtcccc tgttccagag
     2101 gtgcagttcc gccttctc
//
LOCUS       DAOTIL010000483         2117 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminobacteriaceae bacterium isolate AMR_MDS_3026
            NODE_62058_length_2117_cov_3.760912, whole genome shotgun sequence.
ACCESSION   DAOTIL010000483 DAOTIL010000000
VERSION     DAOTIL010000483.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868050
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminobacteriaceae bacterium (wastewater metagenome)
  ORGANISM  Aminobacteriaceae bacterium
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae.
REFERENCE   1  (bases 1 to 2117)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2117)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:19:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,224
            CDSs (total)                      :: 3,198
            Genes (coding)                    :: 3,184
            CDSs (with protein)               :: 3,184
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2117
                     /organism="Aminobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_62058_length_2117_cov_3.760912"
                     /isolate="AMR_MDS_3026"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:3034609"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..274)
                     /locus_tag="P5201_09815"
     CDS             complement(<1..274)
                     /locus_tag="P5201_09815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006583267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body protein"
                     /protein_id="HRV98877.1"
                     /translation="MHRGLYAAASAMIVQESMHDVVANNLANVNTSGFRRRIPVNKSF
                     PEVLMDRIEKKSEDGEIKLIAPPFQLGLKGKFLIGDLSLANVISETY"
     gene            complement(329..1375)
                     /locus_tag="P5201_09820"
     CDS             complement(329..1375)
                     /locus_tag="P5201_09820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015556295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein"
                     /protein_id="HRV98878.1"
                     /translation="MFGTDIGIDLGTATVLMYVKNKGIVLREPSVVAVDLDTGKILAV
                     GYEAKNMVGRTPGSITSVRPLKDGVIADYTMTEAMMHHFMRRVLKGFNRFFRNRVMIC
                     VPSGATDVERRAVLEAAVEVGAKEAYLIEEPMAAAIGANLNVEEPRGKMVVDIGGGTT
                     DIAVISLGGIVVSKSLRIGGDRFDEGIIRYLRKQYNLAIGEQTAENLKIMIGTCLQEA
                     PDTKMILKGRDLVQGLPRQIEVSSASVSMAIGEMVQALIDGVRNVLELTPPELSADII
                     DRGIVLTGGGALLRGLTELITRQTGINCFTADDPMECVALGTGKALAEIDKLQASGRS
                     GILVNRRKGRKKKL"
     gene            complement(1473..>2117)
                     /locus_tag="P5201_09825"
     CDS             complement(1473..>2117)
                     /locus_tag="P5201_09825"
                     /inference="COORDINATES: protein motif:HMM:NF013575.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine synthase A"
                     /protein_id="HRV98879.1"
                     /translation="ISVTAAGRTDAGVHSRGQVISFELEKVWRPDKLLLAMNFHLPPD
                     VSVMKIAHAPQSFDARRSALWREYRYLIWHGNAIPPLLRGLVWWNKFRWNRTETLKAC
                     ALYEGTHDFGAFCKTTERPDRSVRTVHRATLRKKGDLTIFTVRGNAFLTNMVRIMVGN
                     LQAVGYGKKDVEWLASLLSGGARAESAMTAPPEGLYFWRVGYGEGTPFRTNGIE"
BASE COUNT          379 a          629 c          634 g          475 t
ORIGIN      
        1 tataggtttc cgagatgacg ttcgcgaggc tgaggtcgcc gatgagaaac tttcctttga
       61 gcccgagctg gaaaggcggg gcgatgagtt tgatctcgcc gtcctcggat ttcttctcga
      121 ttcggtccat aagaacctcg ggaaacgact tgttgacggg aatccgtctc cggaaccccg
      181 aagtgttgac gttcgcgagg ttattggcca cgacatcgtg catactctcc tgaacgatca
      241 tcgcggatgc ggcggcatag agtccccgat gcatattctt tcctccctcg atgctccgcc
      301 ctggcggaag ttgcttccgc gcggcggatc agagcttctt cttccggcct ttcctgcggt
      361 tcaccagtat gccgctccgc cccgaggcct gaagcttgtc gatctcggcg agggcctttc
      421 ccgtgccgag cgcgacgcat tccatcgggt cgtcggcggt gaagcagttg atgccggtct
      481 ggcgggtgat cagttcggtg aggccccgca aaagagcgcc tcctccggta agtacaatcc
      541 ccctgtctat tatatcggct gagagttccg gcggcgttag ctccaaaaca tttcgaactc
      601 catcaatcag cgcctgtacc atttctccga tggccatcga gacgctcgcg ctggagactt
      661 ctatctggcg aggaagcccc tggaccaggt ctcgcccctt gagaatcatc tttgtatcgg
      721 gagcttcctg aagacaggtg ccgatcatga tcttgaggtt ttccgccgtc tgttcgccga
      781 tggcgaggtt atactgcttg cgcaggtacc ggatgattcc ctcgtcgaag cggtcgcccc
      841 ctatacgaag cgacttggag acgacgatgc ccccgaggga gatgacggcg atatccgtag
      901 tgccgcctcc gatgtcgacg accatttttc cgcggggctc ctcgacgttg aggtttgcgc
      961 cgatcgccgc ggccatgggt tcctcgatga ggtaggcttc tttagcgccg acctcgactg
     1021 cggcttcgag gaccgcgcgg cgctcgacat cggtggcgcc ggaaggaacg cagatcatga
     1081 cccggttgcg gaagaagcgg ttgaagccct tgagcacacg gcgcatgaag tggtgcatca
     1141 tggcttcggt catggtgtag tcggcgatga cgccgtcctt gagcggacgg acggaggtga
     1201 tgctcccggg cgttcgtccg accatattct tggcttcgta ccccaccgcg agaattttcc
     1261 cagtgtcaag gtctaccgcc accacagagg gttcgcggag tacgatgccc ttgttcttga
     1321 cgtacataag gactgttgca gttcccaggt ctattccgat atccgttcca aacacggtgt
     1381 tttcctcctt tggatattga agaggctgtt cgcgtccatc tcgcggcaag gatgcggatg
     1441 tttcccgcag gtccgtgccg tgaacactct ctctattcta taccgttcgt gcgaaatggg
     1501 gtaccctctc cgtacccgac gcgccaaaaa taaagccctt ccgggggcgc agtcatggcg
     1561 gattccgccc gcgctcctcc ggagagaagg gaggcgagcc attccacatc cttttttccg
     1621 taccccacgg cctggagatt gcccaccatg atccgcacca tgttggtgag gaaggcgttg
     1681 ccgcggacgg tgaatatcgt gagatcccct tttttgcgga gcgtcgccct gtggaccgta
     1741 cgaacggacc ggtccggccg ctcggtcgtc ttgcaaaaag cgccgaagtc gtgcgtcccc
     1801 tcgtagagcg cgcatgcctt cagcgtttcg gtccggttcc agcggaattt gttccaccat
     1861 accagccccc gaagcagcgg agggatggcg ttgccgtgcc atatcaggta ccggtactcc
     1921 ctccagagcg cggaacggcg ggcgtcgaag ctttgcggcg catgggcgat cttcatgacg
     1981 ctcacgtcgg gggggaggtg aaagttcatg gcgagcagga gtttgtccgg ccgccagacc
     2041 ttttcaagtt caaaagaaat gacctgcccc cgggaatgga cgcccgcgtc ggtacggcct
     2101 gccgccgtga cggagat
//
LOCUS       DAOTIL010000484         2111 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminobacteriaceae bacterium isolate AMR_MDS_3026
            NODE_62341_length_2111_cov_3.146887, whole genome shotgun sequence.
ACCESSION   DAOTIL010000484 DAOTIL010000000
VERSION     DAOTIL010000484.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868050
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminobacteriaceae bacterium (wastewater metagenome)
  ORGANISM  Aminobacteriaceae bacterium
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae.
REFERENCE   1  (bases 1 to 2111)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2111)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:19:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,224
            CDSs (total)                      :: 3,198
            Genes (coding)                    :: 3,184
            CDSs (with protein)               :: 3,184
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2111
                     /organism="Aminobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_62341_length_2111_cov_3.146887"
                     /isolate="AMR_MDS_3026"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:3034609"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..290)
                     /locus_tag="P5201_09830"
     CDS             complement(<1..290)
                     /locus_tag="P5201_09830"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRV98880.1"
                     /translation="MNERGRSLSFWFAEGGFLLLLTVILLSGALVGAWRVRETDAFLR
                     EELLRTTRVLAETIDIRDVRRLTGSELDLGSPQYESFKNYFRSVMQLYPRSR"
     gene            complement(287..>2111)
                     /locus_tag="P5201_09835"
     CDS             complement(287..>2111)
                     /locus_tag="P5201_09835"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HRV98881.1"
                     /translation="SVRPLLFFGRTFAVILRPGPGFRALYPPRAGWMSLFAGIMVTAV
                     LTMLAVTVANRREELERQVAMRTDELRRSKDELFLMNAALVDTTRKTQEIARRAEAAS
                     EAKGRFLANMSHEMRTPLNGFLGMNRLLLDTRLTEEQREYAVAARKSGEALLTLISDA
                     LDIATIDEGRYRLVEEDFDLDELLSKLETIFSAAAHEKGLRFTSVRPSVPLRLHGDGR
                     CLYRILRSLLDNAVKFTVRGEVRFSVSALEESDRTVRLRFSVEDTGVGIPSEQQGCVF
                     ERFWQGDDSTTKSYQGAGLGLPMAKELAALLGGELEMQSEEGRGTTFSFESLFHKGAS
                     PAAPSIQDESVTGRRILLAEDNPVNRKFALAMLRKLGYSVASAENGKEAVDYLSSNEC
                     DLVVMDVQMPVMDGVEATRMIRSGASGALDPSIPVVALTAYVSPEDRERCMVAGMNAF
                     LPKPAAPEELDAAIRRLLYGEAGGERAETHVPAEYAGKAAPVFDRAAALKRLMGDEDA
                     LAELAEEFIEEMPKDLEKFSALVSEGDAAAAGRCAHSIKGAAAGIGGEALRAAAFEAE
                     KAGKAGDANALLKLEPEVREQYELLEAALRQLISSRGGKNE"
BASE COUNT          388 a          712 c          622 g          389 t
ORIGIN      
        1 aagcgactcc gcgggtacag ttgcatcacc gaacggaagt agttcttgaa gctttcatac
       61 tggggcgacc ctaaatccag ttcgctcccc gtaaggcggc gcacgtcgcg gatgtcgatg
      121 gtctccgcaa gaacgcgcgt agtgcgcagc agctcttcgc ggagaaacgc gtcggtctcg
      181 cgcacgcgcc acgcgccgac caaggcgccc gacaaaagaa taacggtgag cagcaagagg
      241 aatccgcctt cggcgaacca gaaagagagc gatcgtccgc gctcgttcat tcgttctttc
      301 ctccccggct ggagatcagt tgacggagcg ccgcctcaag aagctcgtac tgctcgcgca
      361 cttccggctc caatttcagg agcgcgttag cgtccccggc ctttccggct ttctccgcct
      421 cgaacgccgc ggctctgagc gcctctccgc cgataccggc ggcggcgccc tttatcgagt
      481 gcgcgcacct tcccgcagcg gcggcgtccc cttcggagac cagagcggaa aatttctcca
      541 aatccttcgg catctcctcg atgaactcct ccgcaagctc cgccagcgcg tcctcgtcgc
      601 ccataaggcg tttcagagcg gctgcgcgat cgaagacggg cgctgctttc ccggcgtatt
      661 cggccggcac gtgagtttcg gcgcgctctc ctcccgcttc tccgtaaagc agccggcgta
      721 ttgcggcatc cagttcttcc ggagccgccg gcttcggaag aaacgcgttc attccggcga
      781 ccatgcagcg ctccctgtcc tcaggagaga cataggcagt gagcgcgacg acgggtatcg
      841 acgggtcgag cgcgcccgac gcgccgctcc ggatcatccg cgtggcctcc accccgtcca
      901 tgacgggcat ctgcacgtcc atcaccacca ggtcgcattc gttcgaggag aggtagtcca
      961 cggcttcttt gccgttttca gcggaggcga ccgagtaccc cagcttgcga agcatcgcca
     1021 gcgcaaactt ccggttgaca gggttgtcct ccgcaagcag tattcttctg cctgtcacgg
     1081 attcgtcctg tatggaaggc gccgcagggg aagccccctt gtggaagagc gactcgaaag
     1141 aaaacgtcgt tcccctcccc tcctcgcttt gcatttcaag ctccccgccg agaagcgcgg
     1201 caagctcctt cgccatgggg aggccaagcc ccgcgccctg gtacgacttc gtggtcgagt
     1261 cgtcgccttg ccagaagcgt tcgaagacgc acccctgctg ctccgacggg ataccgacgc
     1321 ccgtgtcctc caccgaaaag cgcaggcgaa cggtccgatc gctctcttca agggcggaga
     1381 cggaaaaacg cacctccccg cggacggtga acttcaccgc gttgtccagg aggcttctca
     1441 gtattctgta aagacagcga ccgtcgccgt gcagccgcag cggaacagac ggacggacgg
     1501 aggtgaaacg aagtcctttc tcgtgcgctg ccgcgctgaa gatcgtttcc agtttcgaaa
     1561 gcagttcgtc caagtcgaaa tcttcctcga cgagacggta gcggccttcg tcgatggtcg
     1621 caatatcaag cgcgtcgctt atcagcgtca gcagcgcctc gccggacttt cgagccgcaa
     1681 cggcgtactc ccgctgctcc tccgtcagac gggtgtcgag cagcagccgg ttcatcccga
     1741 ggaagccgtt caacggcgtc cgcatctcgt gactcatgtt cgcgaggaag cgccccttcg
     1801 cctcgctggc cgcctcggcg cggcgcgcta tctcctgcgt cttgcgcgtc gtatccacga
     1861 gggccgcgtt catcaggaag agttcgtcct tgcttcggcg cagctcatcc gttctcatcg
     1921 cgacctggcg ctcaagctcc tccctgcggt tcgcgactgt aaccgcgagc atggtcagca
     1981 cagcggtcac catgatgccg gcgaaaaggc tcatccaacc ggcacggggc ggatagagcg
     2041 cacgaaagcc gggtccggga cgcagaatga cggcgaacgt cctgccgaag aagagaaggg
     2101 ggcgcaccga g
//
LOCUS       DAOTIL010000485         2110 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminobacteriaceae bacterium isolate AMR_MDS_3026
            NODE_62382_length_2110_cov_2.632603, whole genome shotgun sequence.
ACCESSION   DAOTIL010000485 DAOTIL010000000
VERSION     DAOTIL010000485.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868050
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminobacteriaceae bacterium (wastewater metagenome)
  ORGANISM  Aminobacteriaceae bacterium
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae.
REFERENCE   1  (bases 1 to 2110)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2110)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:19:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,224
            CDSs (total)                      :: 3,198
            Genes (coding)                    :: 3,184
            CDSs (with protein)               :: 3,184
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2110
                     /organism="Aminobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_62382_length_2110_cov_2.632603"
                     /isolate="AMR_MDS_3026"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:3034609"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(70..207)
                     /locus_tag="P5201_09840"
     CDS             complement(70..207)
                     /locus_tag="P5201_09840"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRV98882.1"
                     /translation="MKDVEKTPKMIEKQQRLLYHAFSEAAAEGDVEFASEAQSEILGD
                     D"
     gene            complement(274..1020)
                     /locus_tag="P5201_09845"
     CDS             complement(274..1020)
                     /locus_tag="P5201_09845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006301372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine amidotransferase"
                     /protein_id="HRV98883.1"
                     /translation="MNKRVLIAGESWVKHISHIKGFDIFTSCEYETGITWLKEALEKH
                     GFEVVHLPGHEVADGFPFTAKELAAYGAVALSDIGANSLLLSGDTFNRSKVMPNRLEV
                     VREYVEQGGGFAMIGGYLSFAGIDGKARYADTAIDEILPVKIANFDDRVEKPEGITPV
                     TTEEGHPMMQGIGAWPHFLGYNRTTLRPEGRLLASAGKDPFVAVRLVGKGRTAAFASD
                     CAPHWGPQEFVEWANYPLFWSRFFGWLCGE"
     gene            complement(1017..>2110)
                     /locus_tag="P5201_09850"
     CDS             complement(1017..>2110)
                     /locus_tag="P5201_09850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006301373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ArgE/DapE family deacylase"
                     /protein_id="HRV98884.1"
                     /translation="DGLARFGEGNPGHVYSGDGFSRWNLIGHFKGGEGRSLLFDGHID
                     TMPSGPRELWSFDPLDAQMRDGKIWGLGTCDMKGGLLASVLGVKLLQDAGIPLPGDVH
                     ILSVVDEEGGGNGSLAAMLSGHRADAAVVCEPSNGSVTVAHMGFVFFKVEVRGVALHS
                     GSKWKGVNAIEKAVLLMGALDDLEHEWLMRYKHPLLPPPTLNIGVIEGGTAGSTVPDR
                     CVFKLCLHYHPETMSHDSVVKEVTDRLLLRAAGDPWLRDNPPEISIYQAGGAFEMERE
                     HPFVLAVEESIASVRKEAPPVVGSPAGNDARLLRNIGTMPVVITGPGRQEQCHSPDEY
                     IEANDYLEYIEIYARLILDWCGRHKEERS"
BASE COUNT          356 a          740 c          619 g          395 t
ORIGIN      
        1 tcttttggcc tgctctcacc agttcgacga aaatacccct acaaggcata tctctctccc
       61 cgctgtggac taatcatcgc cgagaatctc gctctgcgcc tcggaagcga attcgacatc
      121 cccctcggcc gcagcttcgc tgaaagcgtg gtacagaaga cgctgttgtt tctcgatcat
      181 cttgggtgtt ttctcgacat ctttcaaaga ctccgccctc ttctcttttt tacgacgaga
      241 gggcggcttt cgccgccctc tttccatcca tccctactcg ccgcagagcc agccgaagaa
      301 gcggctccag aagagcgggt agttcgccca ctcaacgaac tcctgcggcc cccagtgcgg
      361 ggcgcagtcg gaggcgaagg cggccgtgcg ccccttgccg acgagacgga ccgccacgaa
      421 ggggtccttg ccggccgagg ccagcaggcg gccttcgggg cggagcgtcg tccggttgta
      481 ccccaggaaa tgcggccagg cgccgatccc ctgcatcatc gggtgcccct cctccgtcgt
      541 gaccggagtg attccctcgg gtttctccac tctgtcgtca aagttcgcga tcttgacggg
      601 cagtatctcg tcgatagccg tgtcggcata gcgcgccttg ccgtcgatcc ccgcgaagga
      661 gaggtagccg ccgatcatgg cgaagccgcc cccctgctcg acgtactccc ggacgacctc
      721 cagcctgttc ggcatcacct tgcttctgtt gaacgtgtcg cccgagagca gcagcgagtt
      781 ggcccctatg tcggagaggg cgaccgcacc gtacgcggca agctccttcg ccgtgaaggg
      841 aaagccgtcc gcgacttcgt gccccggcag atggacgacc tcgaagccgt gcttctccag
      901 cgcttccttc agccatgtga tgcccgtctc atactcgcag ctcgtgaaga tatcgaaccc
      961 cttgatgtgg gagatgtgct tcacccacga ctctcccgcg atcagcactc gtttgttcat
     1021 gaacgctcct ccttgtgacg cccgcaccag tcgaggatca atcgggcgta gatttcgatg
     1081 tactccagat agtcgttcgc ttcgatatac tcgtccggag agtggcactg ctcctgccgc
     1141 cccggccccg tgatcaccac gggcatcgtg ccgatgttcc gcagcagccg ggcgtcgttc
     1201 cccgccgggg agccgaccac gggcggagcc tctttgcgca cggacgcgat gctctcctcg
     1261 acggcgagca cgaatgggtg ctcccgctcc atctcgaacg cgccccccgc ctggtagatg
     1321 gagatctcag gcgggttgtc ccgcagccac gggtcgcccg cagcgcggag cagcaagcga
     1381 tccgtcacct ccttgaccac ggagtcgtgg ctcatcgtct ccgggtgata gtggaggcag
     1441 agcttaaaga cgcagcggtc gggcacggtc gagcccgccg ttcccccctc gatgacgccg
     1501 atgttgagcg tcggaggcgg gagaaggggg tgcttgtagc gcatcagcca ttcgtgttcg
     1561 aggtcgtcga gcgcacccat cagcagtaca gccttttcga tggcgttgac gcccttccac
     1621 ttgctccccg agtgcagcgc gacgccgcgc acctccacct tgaagaagac gaaccccatg
     1681 tgcgccacgg tgaccgagcc gttcgacggc tcgcagacca ccgccgcgtc cgccctgtgg
     1741 ccggagagca tcgcggcgag agagccgttc cccccgccct cctcgtccac cacggagagg
     1801 atatgcacgt cgccggggag cggaatcccc gcgtcctgca gcagcttcac cccgagcacg
     1861 gaggctagga gccccccctt catatcgcac gtcccgagcc cccatatctt cccgtcgcgc
     1921 atctgcgcat cgagagggtc gaagctccac agctccctcg ggccggacgg catggtgtcg
     1981 atatgtccgt cgaagagaag cgaccgcccc tccccgccct tgaaatgccc gatcaggttc
     2041 caccgggaga agccgtcgcc cgagtagacg tggccgggat tcccctcgcc gaagcgggcg
     2101 agaccgtcct
//
LOCUS       DAOTIL010000486         2107 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminobacteriaceae bacterium isolate AMR_MDS_3026
            NODE_62471_length_2107_cov_4.487817, whole genome shotgun sequence.
ACCESSION   DAOTIL010000486 DAOTIL010000000
VERSION     DAOTIL010000486.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868050
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminobacteriaceae bacterium (wastewater metagenome)
  ORGANISM  Aminobacteriaceae bacterium
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae.
REFERENCE   1  (bases 1 to 2107)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2107)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:19:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,224
            CDSs (total)                      :: 3,198
            Genes (coding)                    :: 3,184
            CDSs (with protein)               :: 3,184
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2107
                     /organism="Aminobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_62471_length_2107_cov_4.487817"
                     /isolate="AMR_MDS_3026"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:3034609"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            175..1728
                     /gene="istA"
                     /locus_tag="P5201_09855"
     CDS             175..1728
                     /gene="istA"
                     /locus_tag="P5201_09855"
                     /inference="COORDINATES: protein motif:HMM:NF033546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS21 family transposase"
                     /protein_id="HRV98885.1"
                     /translation="MSDFLTIVRAVYSGLSQRKVAATHGVSRNTVSLYLRKARDQGWL
                     TLKDLDAVDDNAVAQCLQQIAAPSRDATFKMPDFDYVHAELAKAHVTLKLLWEEYVEQ
                     CRQSNERFYMETQFRRYYHLHAKVHKATIRLEHKPALSLEVDWAGTRIAFFDAESGKM
                     SQASLFVAVLPCSGIIYVEPFCDEKLPSWIAGHVHAFQYLGGVPKTLVPDNLKSGVKR
                     PNFYEPDLNKTYQEMAAYYGTVVLPARVRKPRDKASVENAVLISSRKIIAALRNVQIL
                     SFQDLQQHVRAALEHVNSAPLTGKSESRWTSFLAEEKEFLLPLPESPYELAQWGKAKV
                     QMNCHILCQRKFYSVPFEYLGEDVDVRATQATVEIFYQHQRIASHIRLWGKSDYATVA
                     EHMPPGKLFFADWNRDRFLRWAEKTGVATRRVVEAILDRAVVEQQAYRSCLGVLSLRE
                     KFGPLRLERACGVIASRTSSPTYQQLKNILEKKMDIPRTETPEEGAKKPQGRGFQRGA
                     EYFGGNDHA"
     gene            1721..>2107
                     /locus_tag="P5201_09860"
     CDS             1721..>2107
                     /locus_tag="P5201_09860"
                     /inference="COORDINATES: protein motif:HMM:NF013828.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HRV98886.1"
                     /translation="MLEHETVNKLKYMKLTGIAEALKEQNDDEGYREMGFHDRFALLV
                     DREFCKRQHGRLQRLINGAKFENPTACIEDIRYDDDRKLDRNFILELASCNYVPHARN
                     IVIVGPTGCGKSFLSQALGHAACRRLL"
BASE COUNT          485 a          638 c          609 g          375 t
ORIGIN      
        1 ggaggttcta gagaggtgga aaacacggcg aaaatgagcc gcccgcgcgg agtgttagag
       61 ccattggcgc ggacctgaag agccacccga cgtgccgaca ctaagccggt ctcccgaact
      121 cctgtaagat cgaagtaccg ccggaaagcg gaattcatct caggagggat tacattgtca
      181 gattttctca ccatagttcg ggccgtttac agcggcctga gccagcgcaa ggtcgccgcc
      241 acgcacggag tatccagaaa cacggtgtcg ctgtatctgc gaaaggcacg ggatcagggc
      301 tggctcaccc tgaaggatct ggacgcagtg gacgataacg ccgttgcaca atgcttgcag
      361 caaatcgcgg cgccgtcccg cgacgcgacc ttcaagatgc ccgacttcga ctatgtgcat
      421 gctgaactgg cgaaggcgca cgtcaccctc aagctgctct gggaggagta cgtcgaacaa
      481 tgccgtcaaa gcaacgaacg cttttacatg gagacgcaat tccgccggta ctatcacctg
      541 cacgccaagg tgcacaaggc aaccatccgg ctggagcaca aacccgcgct ctcgctcgag
      601 gtggactggg ccggcacaag gatcgcgttc ttcgacgcgg agagcgggaa gatgtctcag
      661 gcgtcgttgt tcgtcgccgt tctgccgtgt agcgggatca tctatgtcga accgttctgc
      721 gacgaaaaac tgccgtcctg gatcgccggt catgtccacg ccttccaata tctcggcggt
      781 gtccccaaga cgcttgttcc ggacaatctc aaaagcggcg tcaagcggcc caatttctac
      841 gaacccgacc tgaacaagac gtatcaggag atggcagcct attacggaac ggtcgtactg
      901 cccgcgcgcg tccgcaaacc cagggataaa gcgtccgtgg agaacgccgt cctgatttcg
      961 tcccgaaaaa ttatcgccgc tctccgcaat gttcagattc tctccttcca ggatctgcag
     1021 cagcacgtac gtgctgctct ggaacatgtc aactccgccc ccttgaccgg gaagagcgag
     1081 agtcgctgga catcctttct cgccgaagaa aaggagttcc tgctcccgct tcccgaatcc
     1141 ccctacgaac tggcacagtg ggggaaagcc aaggtccaga tgaactgtca cattctctgt
     1201 caacgcaaat tctactccgt tccgttcgaa tacctgggag aagatgtcga cgttcgagca
     1261 actcaagcga cagtggaaat attctaccag catcagagga tcgcgtcgca tatcaggctt
     1321 tgggggaaga gcgactatgc gaccgtcgcg gagcatatgc cccccggcaa gctcttcttc
     1381 gccgactgga accgcgaccg cttcctgcgc tgggcggaga aaaccggcgt cgccacacga
     1441 cgggtggtag aagccattct cgacagggcg gtcgtcgagc agcaggccta ccgctcctgt
     1501 ctcggcgtgc tgagcctgcg ggagaagttc ggcccgctgc ggctcgaacg ggcctgcggc
     1561 gtcatcgctt cgagaacttc atctccgacc taccagcaac tcaagaacat ccttgagaag
     1621 aagatggaca taccccggac cgagacgccc gaagaaggcg cgaagaaacc gcaagggcgc
     1681 ggattccagc gcggggcgga atacttcgga ggcaacgacc atgcttgagc atgaaaccgt
     1741 gaacaagctc aagtacatga agctgacggg gatcgccgag gcgctcaagg agcagaacga
     1801 cgacgaaggc taccgggaga tgggcttcca cgaccgcttc gcgctgctgg tggaccgcga
     1861 gttctgcaaa cggcagcacg gacggttgca gcgcctgatc aacggggcca aattcgaaaa
     1921 tccgaccgcc tgcatcgaag acatccgata cgacgacgac cggaagctcg accgcaactt
     1981 catcttggaa ctcgcgtcgt gcaactacgt cccgcacgca cgcaacatcg tgatcgtcgg
     2041 accgacgggt tgcgggaagt cgttcctgtc ccaggctctc ggacatgccg cctgcagacg
     2101 tctgctg
//
LOCUS       DAOTIL010000487         2106 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminobacteriaceae bacterium isolate AMR_MDS_3026
            NODE_62523_length_2106_cov_3.684057, whole genome shotgun sequence.
ACCESSION   DAOTIL010000487 DAOTIL010000000
VERSION     DAOTIL010000487.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868050
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminobacteriaceae bacterium (wastewater metagenome)
  ORGANISM  Aminobacteriaceae bacterium
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae.
REFERENCE   1  (bases 1 to 2106)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2106)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:19:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,224
            CDSs (total)                      :: 3,198
            Genes (coding)                    :: 3,184
            CDSs (with protein)               :: 3,184
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2106
                     /organism="Aminobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_62523_length_2106_cov_3.684057"
                     /isolate="AMR_MDS_3026"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:3034609"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            2..1564
                     /gene="ilvD"
                     /locus_tag="P5201_09865"
     CDS             2..1564
                     /gene="ilvD"
                     /locus_tag="P5201_09865"
                     /EC_number="4.2.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005658699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004160 - dihydroxy-acid dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009097 - isoleucine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009099 - valine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxy-acid dehydratase"
                     /protein_id="HRV98887.1"
                     /translation="MGVVNPYNALIPGHIHLNRIAEAVKSAVYAFGGFPLEFPVIGVC
                     DGIAMNHEGMKFSLPSRELIMDSIEVMVRAHALDALVLVTNCDKIIPGMAMAALSLNI
                     PTIMISGGPMLAGTTGGKDVDLSNIFEAVGKRAAGLISDEELREIEDITCPTCGSCAG
                     MFTANTMNCMIEALGFALPGNGTIPAVYSARDRLAKEAGRQIMTLVERNIKPRDILTE
                     KAFDNAVAVDMALGGSTNTALHLPALAHAAGLSLTLDRFDEISARTPHLCSMSPGGPH
                     HIQDLYAAGGVQAVMARLVQGGLIDGNSLTVTGKTVAENLEGVVVKDDAVIRPLDRPY
                     HAQGGLAVLKGNIAPEGSVVKQSAVDPSMLKHSGPARVFDSEEAASKTILEKGIKDGD
                     VVVIRYEGPKGGPGMREMLTPTSSLAGMGMDKTVALITDGRFSGASRGASIGHVSPEA
                     AAGGMIGLVKEGDIIEIDIPARKLHLAVPEDEIARRRSEWKPVETQVDSLFLNRYREF
                     VTSGSKGAVFSD"
     gene            1630..>2106
                     /locus_tag="P5201_09870"
     CDS             1630..>2106
                     /locus_tag="P5201_09870"
                     /inference="COORDINATES: protein motif:HMM:NF012655.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR family transcriptional regulator"
                     /protein_id="HRV98888.1"
                     /translation="MGERENTEARILDAVGTILSRKGFRYLGVNAIAREACVDKVLIY
                     RYFGGLQPLLRTFFTRRKYWPTTSEILETAGEGEADPATRAYAILRGYLRELKKSDAA
                     REVLRGEIAGREDVASETAEERYRQGMELLDLLREPDKETEGLTEMAALLSAGFTFL"
BASE COUNT          434 a          600 c          661 g          411 t
ORIGIN      
        1 ggtgggcgtg gtcaacccct ataacgcgct tattcccggt catattcatc tgaaccggat
       61 cgcggaggca gtgaaatccg ctgtgtacgc attcgggggc ttccctctgg aattccccgt
      121 catcggcgtc tgcgacggca tcgccatgaa tcacgagggg atgaagttct cgctccccag
      181 ccgcgagctg atcatggatt ccatcgaggt gatggtgcgg gcgcatgcgc ttgacgctct
      241 cgttctcgtc accaactgcg acaagattat ccccggcatg gcgatggcgg cgctctcgct
      301 gaacatcccg acgatcatga tcagcggcgg gccgatgctc gccggaacga cgggaggcaa
      361 ggacgtcgat ctcagcaaca tcttcgaggc tgtcggcaag agggccgccg ggttgatttc
      421 cgacgaggag ctccgcgaga tagaggatat cacctgtccg acatgcggtt cgtgcgccgg
      481 tatgttcacc gcgaatacca tgaactgcat gattgaggcg ctcggttttg ctctccccgg
      541 gaacggaacc attcctgccg tctactcggc ccgggaccga ctggccaagg aggccggacg
      601 gcaaatcatg acgctggtcg agaggaacat caagcccaga gacatcctca cggagaaggc
      661 cttcgacaac gccgttgccg tcgatatggc gctcggcggc tctaccaaca ctgcgctcca
      721 tcttccggca ttggcgcatg ccgcaggtct ttcgctgacg ctcgacaggt tcgacgagat
      781 cagcgctcgt acgccgcatc tgtgcagcat gagccccggc gggccgcacc atatccagga
      841 cctctacgcc gcaggcggag tccaggccgt gatggcgcgt cttgtgcagg gcggtcttat
      901 cgacgggaac tcgcttacag taacagggaa gaccgtagcc gaaaacctcg agggggtcgt
      961 tgtaaaggac gacgctgtta tccgtccgct cgacaggccg taccacgccc agggcggtct
     1021 tgccgtgctt aagggaaaca tcgctccgga ggggagcgtt gtcaagcagt ccgccgttga
     1081 tccttcaatg ttgaagcact ccggtccggc ccgcgtcttc gacagcgagg aagcggcgtc
     1141 caagaccatt cttgagaagg ggatcaagga cggcgacgtg gttgtcatcc gttacgaagg
     1201 ccccaagggc ggaccgggga tgcgcgagat gcttactccg acctcttcgc tcgccggaat
     1261 ggggatggat aagaccgttg cgctcatcac cgacggacgg ttctccggcg cttcacgcgg
     1321 cgcatctatc ggtcatgtct ctccggaggc ggctgcgggc ggaatgatcg gcctcgtgaa
     1381 agaaggagac atcatcgaga tcgacatccc cgcacggaaa ctgcacctcg ccgttcctga
     1441 agacgagatc gcccgccgca gatcggaatg gaaacctgta gagacccaag tcgacagtct
     1501 tttcctcaac aggtatcgcg agtttgttac atccggctcc aaaggagcgg tgttcagcga
     1561 ctaatgattt ttgctgtgaa ggggctttct ccggaagccc cttctcgctt tccaaagggg
     1621 gaaaagaatg tgggggagag ggagaatacc gaagcccgca tcctcgacgc ggtagggacc
     1681 atcctttcaa gaaaaggttt ccggtacctt ggggtgaacg ccattgcccg ggaagcttgt
     1741 gtggacaagg tgctcatcta tcgttatttt ggagggcttc aaccgcttct gcggacgttt
     1801 ttcacgcgga ggaagtactg gccgacaacc tcggaaatcc tggagaccgc cggtgaaggc
     1861 gaggccgatc cggcgacgag ggcgtacgcg atcctcaggg ggtatctccg ggagctgaaa
     1921 aagtcggatg ctgcccgtga agttctgcgg ggagaaattg caggaagaga agacgtcgct
     1981 tcggaaacgg cggaagaacg ttaccggcag gggatggagc tgctcgacct tcttcgtgaa
     2041 cccgataagg agacggaagg actgaccgag atggccgcgc tcctctctgc gggattcact
     2101 ttcttg
//
LOCUS       DAOTIL010000488         2099 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminobacteriaceae bacterium isolate AMR_MDS_3026
            NODE_62793_length_2099_cov_4.035714, whole genome shotgun sequence.
ACCESSION   DAOTIL010000488 DAOTIL010000000
VERSION     DAOTIL010000488.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868050
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminobacteriaceae bacterium (wastewater metagenome)
  ORGANISM  Aminobacteriaceae bacterium
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae.
REFERENCE   1  (bases 1 to 2099)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2099)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:19:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,224
            CDSs (total)                      :: 3,198
            Genes (coding)                    :: 3,184
            CDSs (with protein)               :: 3,184
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2099
                     /organism="Aminobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_62793_length_2099_cov_4.035714"
                     /isolate="AMR_MDS_3026"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:3034609"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(151..1371)
                     /locus_tag="P5201_09875"
     CDS             complement(151..1371)
                     /locus_tag="P5201_09875"
                     /inference="COORDINATES: protein motif:HMM:NF018682.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="four-carbon acid sugar kinase family protein"
                     /protein_id="HRV98889.1"
                     /translation="MNGFAVLCDDLTGSSVQSILLKDRGVPVRQIIRLSGSPPSPPGE
                     GEALVINCDTRRRSREEAEKIFMEILGSFPPDIGIAKRIDTTLRGHLGSETEAILKTR
                     PGAVAMVVPAYPSSGRTTVGGYHLLNGSLLERTEVARDPLWPISTSYVPSYFKGLLVA
                     RMHMECVKRSEEEVIVELRKLLSEGARVVVADAMTDSDIEKLASASFASKEDIIPVDP
                     GPFTAALLSRHLSRRSPGTVLAVIGSTSEKTRKQIAWLDGKIPSEEFVFSPSETTEKA
                     ASRLREFLTSLDRTVSFLLLRPDPRVEKGSEERTARTLAFLGKEALTVLGGHVRGILL
                     SGGDTAAMFFEAAGATALLPETEIQPLMMGGKILDGAFAGLKTVTKGGLVGEEDAIYK
                     AALWLRLAPEATRP"
     gene            complement(1376..>2099)
                     /locus_tag="P5201_09880"
     CDS             complement(1376..>2099)
                     /locus_tag="P5201_09880"
                     /inference="COORDINATES: protein motif:HMM:NF018513.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="TRAP transporter large permease subunit"
                     /protein_id="HRV98890.1"
                     /translation="VVMQSIKLDIPKEPAREKGELLAAVKDSILAIIMPVIILGGIYG
                     GVFTPTEAGAVAVIYSLLVSAFIYEELDLRTIRTVIIKAGISTSVVFFVIATSQSMSW
                     LITVSRVSAELAAWIVSVSSNPFVLVTMINFILLFLGVFLETQAIILLMAPLLLPITA
                     SIGMNPLMLGIIMVVNTSVGMITPPMAVNLFVAVSLVKDHNVRIEQITRKILGFLAAE
                     LIAIMLISNFPVLSLGLVQLLK"
BASE COUNT          446 a          619 c          614 g          420 t
ORIGIN      
        1 cgagcagcgg cgttccacgc tcccaaactt cagcatcagc catgctcgca agggcgatct
       61 ccgggccgac tcctgcgggg tcgcccatgc tgactgcgat cactgtacgt ttcatgaaat
      121 attactccct ttccttcccc ctccgctctt tcagggacga gttgcttcgg gggcgagtct
      181 caaccagaga gcggccttgt aaattgcgtc ttcttcgccg acgaggccgc ccttggtgac
      241 tgttttcaaa ccggcgaacg caccgtcgag aattttgccg cccatcatca gcggctgtat
      301 ctcggtttcg ggaaggagcg cggtcgctcc ggccgcctcg aagaacattg ccgccgtgtc
      361 tcctccggag agaagtatac cccgaacatg acctccgagt acagtaaggg cttctttccc
      421 gaggaaggcc agggtacggg cggtccgctc ctccgagcct ttttcgacgc gcggatccgg
      481 tcgaagcagg aggaatgaga ccgtcctgtc caaactcgtc agaaactcgc ggagacgaga
      541 tgcagccttc tccgtcgtct ccgaaggcga gaagacgaac tcttccgacg gaatcttgcc
      601 gtcgagccag gcgatctgtt ttctggtctt ctccgacgta cttccgatga ctgcgagcac
      661 agtacccgga cttctccggg agagatggcg cgaaaggaga gccgccgtga aagggcccgg
      721 gtccacaggt atgatatcct ccttggaggc aaacgaggct gatgccagct tttcaatatc
      781 gctgtccgtc atggcgtccg ccacgacgac gcgcgcccct tcagagagaa gtttcctcag
      841 ctcgacgatg acttcttcct cgctcctctt gacgcactcc atgtgcatcc gggcgacgag
      901 gagccccttg aagtaggatg gaacgtagct ggtagagatc ggccagaggg gatcgcgggc
      961 gacttctgtc cgttccagca gggagccgtt caaaagatgg taccctccga ccgtcgtcct
     1021 cccggaggag gggtaggcgg ggacgaccat ggcgactgct ccggggcggg ttttcaggat
     1081 cgcctccgtt tcgctcccga ggtgtcctct gagcgtggtg tcgatacgtt ttgcgattcc
     1141 aatatccggc ggaaaagacc ccagaatctc catgaagatc ttctcggcct cttcccgtga
     1201 tcgtcgccgc gtatcacagt tgatcaccag cgcctccccc tccccgggag gagaaggcgg
     1261 actgccggag agacgtatta tctgacggac gggaactccg cggtccttca acaaaatgga
     1321 ctggacggaa gaaccggtga ggtcgtcgca gagcacagca aagccgttca tagcgctatt
     1381 tcaacagctg aacaagcccc agggaaagaa cggggaaatt actgatgagc atgatcgcaa
     1441 tcagctccgc cgcgaggaag ccgagaattt tccgcgtgat ctgctctatg cgcacgttat
     1501 gatccttcac cagcgagacc gcgacgaaca ggttcaccgc cattggcgga gtgatcatcc
     1561 ctaccgaggt gttcaccacc atgatgatcc cgagcatcag cggattcatg ccgatggacg
     1621 cggtgatggg aaggagcagc ggcgccatca gcaggatgat cgcctgcgtc tccaggaaga
     1681 cgccgaggaa gaggaggata aagttgatca tcgtaaccag gacaaacggg ttcgacgaca
     1741 cggaaacgat ccatgcggcc agctccgccg ataccctgct caccgtgatc agccacgaca
     1801 tcgactgcga cgtggcgatg acgaagaaga cgacggaagt actgatgccc gctttgatga
     1861 tgaccgtccg gattgtccgc agatcaagtt cttcgtatat gaacgcgctg actaaaagac
     1921 tgtagatcac cgcgaccgcg cccgcctcgg tcggggtgaa gaccccgccg tagatgccgc
     1981 cgaggatgat gacaggcatg atgatggcga gaatggagtc cttgactgca gcgaggagtt
     2041 ctcccttttc tcttgcaggt tctttcggga tgtcgagctt gatggactgc atcaccacg
//
LOCUS       DAOTIL010000489         2098 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminobacteriaceae bacterium isolate AMR_MDS_3026
            NODE_62847_length_2098_cov_2.644640, whole genome shotgun sequence.
ACCESSION   DAOTIL010000489 DAOTIL010000000
VERSION     DAOTIL010000489.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868050
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminobacteriaceae bacterium (wastewater metagenome)
  ORGANISM  Aminobacteriaceae bacterium
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae.
REFERENCE   1  (bases 1 to 2098)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2098)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:19:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,224
            CDSs (total)                      :: 3,198
            Genes (coding)                    :: 3,184
            CDSs (with protein)               :: 3,184
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2098
                     /organism="Aminobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_62847_length_2098_cov_2.644640"
                     /isolate="AMR_MDS_3026"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:3034609"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1353
                     /locus_tag="P5201_09885"
     CDS             <1..1353
                     /locus_tag="P5201_09885"
                     /inference="COORDINATES: protein motif:HMM:NF012706.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HRV98891.1"
                     /translation="ELRQAAWQALPRERVVRDLFAGYAEPLSTFLPPVSPYYEPNVAA
                     YPFDPDAAKKRLADAGWTWGEDGVLISPEGEKIPDQRLLSPTASVAPTTAELAARAAD
                     ALSAIGIPVRSEPMDFSTMLSRLDERDFQMYVLAWQMTRDPDSLYAFYSTKMDIPGGY
                     NLPGISDPELDAVLDRLRFAPDAMTALDAASDAQKKLAELAPVAPIYSRYTISAVRRD
                     WKGTLVTDRTTADNLWSLLSMEPAAGPMRPLYLVQGEEPRALNPFTSSSAYDWQVLGL
                     LYDSFIAIDPYTLETIPWLAKSWTVTTKTRDDGLHTVLTFHLRDNVRWQDGKPMTAED
                     AAFTYEYLRKNMIPRYFDNVDNIEKIETPDPLTLVVTMKNTSFWHLHRVGGIPVLPRH
                     IVEKVEDWRSWLPASLKHPEDDSLTQLVGTGPFVFREYRAGEYVHLTANDAFWLGRKR
                     "
     gene            1361..>2098
                     /locus_tag="P5201_09890"
     CDS             1361..>2098
                     /locus_tag="P5201_09890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013049153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HRV98892.1"
                     /translation="MNYWGRRISGALLVLALVLVLNFLLFRLMPGDPVASIVDPRFSP
                     EAKEQLRRMYGLDQPLPVQFALYVKSTLTFDFGISFLTGLPVAEELRSRVPNTVILLG
                     TSLLLSAALGTWLGMKAALRRGSLLERCVLWSGALSFSFPSFFVQLLLLMFFASMIPL
                     FPLRGTLSVPPPAGGPALLFDYLRHMTLPVFSLVLLGFGGWAIYVRNLTVKILGEDFI
                     LLARARGLPKKNILRGHVFRTMLPPLLT"
BASE COUNT          342 a          715 c          647 g          394 t
ORIGIN      
        1 gaactgcggc aggcggcgtg gcaggccctt ccgagggagc gcgtcgtgcg cgatcttttc
       61 gcggggtacg cggagccgct ctcgacgttt cttcccccgg tctccccgta ctacgagccg
      121 aacgtcgcgg cgtatccgtt cgaccccgac gcggcgaaga agcgcctcgc agacgccggg
      181 tggacatggg gggaggacgg ggtgctgatc tccccggaag gtgagaaaat ccccgaccag
      241 cgtcttctct cgccgacggc ctccgttgcg ccaaccactg cggagctcgc ggcgcgggct
      301 gcggacgcgc tttcggcgat cggcattcct gtgcgttcgg agccgatgga cttttccacc
      361 atgctctcgc ggctcgacga gcgggacttc cagatgtacg tcctcgcgtg gcagatgacg
      421 cgcgaccccg actctcttta cgcgttctac tcgaccaaga tggacatacc cggcggatac
      481 aacctccccg gcatctccga ccccgaactg gacgccgtgc tggaccggct ccgtttcgcg
      541 cccgacgcga tgacggcgct cgacgcggcc tccgacgccc agaagaagct ggcggagctc
      601 gcccccgtcg cgccgatcta cagccgctac accatctccg ccgtgcgccg ggactggaag
      661 ggaacgctgg tcacggaccg gaccacggcg gacaacctgt ggagcctgct ctccatggag
      721 ccggccgcgg ggccgatgcg cccgctctac ctggtccagg gggaggaacc gcgagcgctc
      781 aacccgttca catccagctc cgcctacgac tggcaggtac tcgggctcct ctacgactcc
      841 ttcatcgcaa tcgaccccta cacgctggag acgattccat ggctcgccaa atcatggacc
      901 gtaacgacaa aaacacggga cgacggactg cataccgtgc tcaccttcca tctgcgcgac
      961 aacgtgcgct ggcaggacgg gaaaccgatg acggcagagg acgccgcctt tacctatgaa
     1021 tacttgcgga agaacatgat tccacggtat ttcgacaacg tggacaacat cgagaagatc
     1081 gaaacccccg acccgctgac gctggtcgtg acgatgaaaa acacgagctt ctggcatctg
     1141 caccgggtcg gcggtattcc ggtgcttccg cggcatatcg tggagaaggt ggaggactgg
     1201 cgaagctggc tgcccgcctc gctgaagcat ccggaggacg actccctgac gcagctcgtg
     1261 gggacgggcc cgttcgtctt ccgcgagtac cgggcgggcg agtacgtcca tctgacggcg
     1321 aacgacgcct tctggctcgg gcggaaaagg taagacgccg gtgaactact ggggaaggag
     1381 aatctcggga gcgctgcttg tcctggcgct cgtactcgtc ctgaatttcc tgctcttccg
     1441 cctcatgccg ggcgaccccg tggcttccat tgtggacccc cgtttctcgc ccgaggcgaa
     1501 ggagcagctg cggcggatgt acggactcga ccagccgctt cccgtgcagt ttgcgctcta
     1561 cgtgaagtcg acgctgacct tcgacttcgg catctccttc ctgacggggc tgccggtggc
     1621 cgaggagctg cgatcccgcg tgccgaacac ggtgattctg ctggggacga gccttttgct
     1681 ctccgccgcc ctgggaacct ggctcggcat gaaagcggcg ctgcggcggg gcagccttct
     1741 cgaacgctgc gtcctctgga gcggcgcgct ctccttctcc tttccctcgt ttttcgttca
     1801 gctgctgctc ctgatgttct tcgcctcgat gatcccgctc tttccgcttc gcgggacgct
     1861 ctccgttcct ccgcccgctg gaggtcccgc gctgctgttc gactacctgc ggcacatgac
     1921 gctgcccgtc ttctcgctcg tgctgctcgg gttcggcggg tgggcgatct acgtgcgcaa
     1981 cctgacggtg aagatcctcg gcgaggactt cattctcctc gcgagggcgc gcggcctgcc
     2041 gaagaaaaac atccttcgcg gacacgtctt cagaacgatg ctcccgcccc tgctcacc
//
LOCUS       DAOTIL010000490         2097 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminobacteriaceae bacterium isolate AMR_MDS_3026
            NODE_62871_length_2097_cov_3.800686, whole genome shotgun sequence.
ACCESSION   DAOTIL010000490 DAOTIL010000000
VERSION     DAOTIL010000490.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868050
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminobacteriaceae bacterium (wastewater metagenome)
  ORGANISM  Aminobacteriaceae bacterium
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae.
REFERENCE   1  (bases 1 to 2097)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2097)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:19:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,224
            CDSs (total)                      :: 3,198
            Genes (coding)                    :: 3,184
            CDSs (with protein)               :: 3,184
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2097
                     /organism="Aminobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_62871_length_2097_cov_3.800686"
                     /isolate="AMR_MDS_3026"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:3034609"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(183..821)
                     /locus_tag="P5201_09895"
     CDS             complement(183..821)
                     /locus_tag="P5201_09895"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HRV98893.1"
                     /translation="MKNCPGCGAVIQEDAAFCEYCGVAVKQRSEAGKEGSASSWPVEG
                     TWSAAPEKTSGASFPQARLKKVSPLIMIFFTLLTFFLYPVVWLFLRLRTFNSMSEKTR
                     IHPVLSGLFACAAVALAVILNGDSLIGPAVPQQAIDTAISYIFLANFILSAVLTFTLR
                     RILREYAARMDKSPLAAKIVARSEVFAFLFQFLYIQHHINLLIDAGLVERTN"
     gene            999..1640
                     /locus_tag="P5201_09900"
     CDS             999..1640
                     /locus_tag="P5201_09900"
                     /inference="COORDINATES: protein motif:HMM:NF012655.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HRV98894.1"
                     /translation="MRSRSRKLDEEKRRNLMDAAIEEIAANGIEGASYNRIIERSGLS
                     KGVVYYYFENKESLYKTVLEEVERQFIAAVGTLRIPDDKDAFWDVCDMHYTKALKFAA
                     SNLRIVDVARSFIEPACSSSMDKGPVFESLQRAERWTRCVLRKGQELGALRQDVDFEL
                     LRNVVQAVEYTMDSWLFDKLRSEPDAVDIERFVSFALDMFKRILSPKGLGESC"
     gene            1634..>2097
                     /locus_tag="P5201_09905"
     CDS             1634..>2097
                     /locus_tag="P5201_09905"
                     /inference="COORDINATES: protein motif:HMM:NF019448.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin/lipoyl-binding protein"
                     /protein_id="HRV98895.1"
                     /translation="MLKKFFSRLSVLFWVVLLCAGGVLMVLRVQARLEESAANAAAME
                     QQEETAVPVDVQKLVRRDWEVWRSYYGQAKAGRSQTVSSYVKEIVRDVHVRIGDKVKA
                     GQVLLTLSSQDQRATEAALRSSYEDSVREYQRLSELQKSGGVSKSQVEQALSR"
BASE COUNT          465 a          523 c          670 g          439 t
ORIGIN      
        1 acgctcgatc tcggggagga tcttcccgag catgctccgg gaaaacttct tatcgacagg
       61 atcaaccgtc tgggctttcc cgcggaggac ggaatgaaat aaacacaagg gagcctcggc
      121 ggatgtcgcc atccgaggct cccttgcgtc agaccggccg ttccgccggg ggcggaagcg
      181 aatcagttcg tccgttcaac gagccctgcg tcgatcagca ggttgatgtg gtgctggata
      241 tatagaaatt gaaagaggaa tgcgaagacc tccgagcggg cgacgatttt ggctgccagg
      301 gggctcttgt ccatgcgtgc cgcgtactcc ctcagaatcc gccgcagggt gaaggtgaga
      361 acagccgaaa gaatgaagtt cgcaaggaaa atatagctga tggcagtgtc gatggcttgc
      421 tgcggtactg ccggtccgat gagcgagtcc ccgttgagaa tcactgccag ggcgactgcg
      481 gcgcatgcga aaaggccgga aagaacagga tggatacgcg tcttctccga catggaatta
      541 aaggtccgaa gccggagaaa gagccagacg acagggtaga gaaaaaacgt cagcagcgtg
      601 aagaagatca taatgagcgg gctgaccttc ttgagcctcg cttgaggaaa ggaagcgccg
      661 gacgtttttt ccggggccgc gctccacgtt ccttcgacgg gccaggacga agcggaaccc
      721 tctttccccg cttcggagcg ctgcttcaca gcgactccgc agtactcgca aaacgcggcg
      781 tcctcctgga tcacggcgcc gcatcccggg cagttcttca tagtgcatcg ctccttgaaa
      841 aagaatcgga ctgtttcatg ccttcgtatc ataccacagg cttcagaggc ttggatgtat
      901 gtgaaattcc agttgatcga ttctcctctt gacaaagtag accggatggt ctaacctgtc
      961 agtcgcactt cacggtcgaa gtgttgagga ggtgcgatgt gcgttcacgt tccagaaaac
     1021 tggatgaaga aaaaagaaga aaccttatgg acgcggccat cgaggagata gccgcgaacg
     1081 gtatcgaggg ggcgtcctat aacaggatca tcgagcgttc cggattaagc aagggagtgg
     1141 tgtactacta cttcgagaac aaggagagcc tgtacaagac ggtgctcgaa gaggtggaac
     1201 gtcagtttat cgccgctgta ggaacgttga gaattcccga cgacaaggac gctttttggg
     1261 atgtgtgcga tatgcactac acgaaggcgc tgaagtttgc cgcttccaat ctgcggatag
     1321 tcgacgtcgc ccgcagtttt atcgagccgg cctgcagctc ctctatggac aaggggccgg
     1381 tttttgaatc tctccagcgg gcggagcgct ggacaagatg cgtgctccga aagggacagg
     1441 agttgggagc gctgcgtcag gacgtcgatt ttgaacttct ccggaacgtg gtgcaggctg
     1501 tggagtacac gatggactcg tggctgttcg acaagttgcg aagcgaaccg gatgcagtgg
     1561 atatagagcg ttttgtgagc tttgcgttgg atatgtttaa aaggattttg tcgccgaaag
     1621 ggttggggga gtcatgctga aaaagttttt ttcgaggttg agcgttcttt tctgggtcgt
     1681 acttctttgc gccgggggcg tcctgatggt gctccgcgtt caggcgaggc ttgaagagtc
     1741 cgcggccaac gccgcagcta tggagcagca ggaggagacg gccgttccgg tcgatgtgca
     1801 gaagctcgtt cgtcgcgact gggaggtctg gcggagctac tacggccagg cgaaggccgg
     1861 gcgcagccag acggtttctt cctacgtcaa ggagatcgtc cgtgatgttc atgtgcgcat
     1921 cggcgacaag gtgaaggcgg gacaggtgct cctgacgctc tcctcgcagg accagcgcgc
     1981 caccgaggct gcgctccgtt ccagttacga ggattccgta agggagtacc agcgtctgtc
     2041 cgagcttcag aaatcgggcg gcgtctccaa gtcgcaggtg gagcaggcgc tctcccg
//
LOCUS       DAOTIL010000491         2095 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminobacteriaceae bacterium isolate AMR_MDS_3026
            NODE_62952_length_2095_cov_3.925000, whole genome shotgun sequence.
ACCESSION   DAOTIL010000491 DAOTIL010000000
VERSION     DAOTIL010000491.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868050
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminobacteriaceae bacterium (wastewater metagenome)
  ORGANISM  Aminobacteriaceae bacterium
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae.
REFERENCE   1  (bases 1 to 2095)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2095)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:19:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,224
            CDSs (total)                      :: 3,198
            Genes (coding)                    :: 3,184
            CDSs (with protein)               :: 3,184
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2095
                     /organism="Aminobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_62952_length_2095_cov_3.925000"
                     /isolate="AMR_MDS_3026"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:3034609"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(347..1108)
                     /locus_tag="P5201_09910"
     CDS             complement(347..1108)
                     /locus_tag="P5201_09910"
                     /inference="COORDINATES: protein motif:HMM:NF015168.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF255 domain-containing protein"
                     /protein_id="HRV98896.1"
                     /translation="MSNAKNRLAKEKSPYLLQHAENPVDWYPWGEEAFAKARTEDKPL
                     FVSIGYATCHWCHVMERESFDDGEVASLLNDACVPVKVDREERPDVDGAFMAVCQMMN
                     GNGGWPLNTFITPQGLPFFASTYLPKRGGGKYPGLVDVVPRVKWLWKTQREQVEQSAK
                     SIRDSLLKEEAPSSGSLPGAAQFKAAYDGLRAGYDREFGGFSKEPKFPMPSHLLFLLR
                     YWKRFGEENAWSMAESSIERCEMKVHMRRGPVRTS"
     gene            complement(1101..1748)
                     /locus_tag="P5201_09915"
     CDS             complement(1101..1748)
                     /locus_tag="P5201_09915"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013049019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HRV98897.1"
                     /translation="MFSCVIFDFDMTLVDSSYAIRDTMNMVAEREGLPKVTREDVLSV
                     IGLPIRDSWLKVWGKFDERWLADFREMFVQQEFAGIFPFSGTKQVLETLAARGVSLGI
                     ASNRQRPGGPLKAVGLESYFSSVVGMDDVEKGKPAPDMLLKGMTDLGGTPATTLYVGD
                     TADDMTAAKDAGIRSVGLTTGNFTPEALHAAGAWKVYDAVEEILPLFGTEGGRSE"
     gene            complement(1855..>2095)
                     /locus_tag="P5201_09920"
     CDS             complement(1855..>2095)
                     /locus_tag="P5201_09920"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="pyruvate ferredoxin oxidoreductase"
                     /protein_id="HRV98898.1"
                     /translation="GTEKETSAEDVAAACARLGVRVIPVRTELPDVRMQNVALLAVAA
                     KEKLIPGVGVEHYEKALADLLPERALQGNLKVLKG"
BASE COUNT          369 a          630 c          665 g          431 t
ORIGIN      
        1 atacgaccat tttcaatcgc aattcatcac tcagagagtg atggctactt ttgtcacacg
       61 tgtctgctga acattgcgct ccggtgcatg gcaccacctg cgtagagtgt gcgccgactg
      121 tgaagatttc atcgaaagtg cagatctaat tggggaatgc cttgtgattg cccatcgtgc
      181 gcgttagttt cgcttgcctg gcggtacttt gagaatggca attgcgggat ttgatgatgg
      241 cgtcaccaga tggagcaccc gatttttcca aggcaattgc tgctctcgat gattgctcag
      301 agcttaccgg gtcagggatt atcatctcat tgtccaacgg caaggtttac gaagtgcgca
      361 ccgggccgcg ccgcatgtgg accttcatct cgcagcgctc gatggagctt tcggccatgg
      421 accatgcgtt ctcctcgccg aaacgcttcc agtagcggag gaggaagagg agatgcgacg
      481 gcatggggaa cttcggctct ttggagaagc ctccaaactc ccggtcgtac ccggcgcgga
      541 ggccgtcgta ggcggctttg aactgggctg ctcccggaag ggagcctgaa gacggagcct
      601 cttccttcag cagggagtcg cggatgctct tcgcggactg ctccacctgt tcgcgctggg
      661 tcttccacag ccatttcacc ctcgggacga catccacaag gccggggtat ttgccgcctc
      721 cccgcttggg gaggtaggtg gaggcgaaga aggggagccc ctgcggggta atgaaggtat
      781 tgagcggcca gccgccgttg ccgttcatca tctggcagac ggccatgaag gcgccgtcga
      841 cgtcggggcg ctcctcgcgg tcgaccttca ccgggacgca ggcgtcgttg agaaggctgg
      901 caacctcgcc gtcgtcgaac gactcgcgct ccatgacgtg gcaccagtgg caggtggcgt
      961 acccgatgga gacgaagagc ggcttgtcct ccgtcctggc tttcgcgaag gcttcctctc
     1021 cccaggggta ccaatcgacg gggttctccg cgtgctggag caggtagggg gatttttcct
     1081 ttgcgagacg gttttttgcg ttactcactg cggccgccct ccgttccgaa gagggggaga
     1141 atctcttcca cggcgtcgta gaccttccac gcgcccgcgg cgtgaagggc ttcgggcgtg
     1201 aagttgccgg tggtcagtcc gaccgaacgg attccggcgt ctttggcggc ggtcatgtcg
     1261 tcggcggtgt cgccgacgta gagagtggtc gcgggcgtgc cgccgaggtc ggtcattccc
     1321 ttgaggagca tatcgggggc gggcttgccc ttctccacat cgtccattcc caccacggag
     1381 ctgaagtagc tctccaaccc gacggccttc aaggggcctc cgggtcgctg acggttggag
     1441 gcgatgccga gggagacgcc gcgagccgcg agcgtttcga gcacctgctt cgtcccggag
     1501 aagggaaaga ttcccgcgaa ctcctgctga acgaacatct cgcggaagtc ggcgagccag
     1561 cgctcgtcga atttacccca gaccttcagc caggaatcgc gtatcggcag accgatgacg
     1621 gagagcacgt cctcccgcgt gactttgggg agcccctccc gctcggcgac catgttcatc
     1681 gtgtcccgta tggcgtagct gctgtccaca agggtcatgt cgaagtcgaa gatgacgcat
     1741 gaaaacatcg aatccactcc ttttctcgaa tcgtccaggg aaatggtact ctatgggtcg
     1801 gagaaaagca aaggccgccc ccgcaggaac ggcctttgcg tacagccggc ttcgctaccc
     1861 tttgagcacc ttcaggttgc cctggagcgc ccgctcggga agcaggtcgg caagcgcctt
     1921 ctcgtagtgt tcgacgccga cgccgggaat cagcttctct ttggccgcga cggcgagcag
     1981 cgcgacgttc tgcatccgca cgtccggcag ctccgtccgt acggggatca cgcgcacgcc
     2041 gagacgggcg caggccgcgg ccacgtcctc cgcggaggtc tccttctccg tgccg
//
LOCUS       DAOTIL010000492         2094 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminobacteriaceae bacterium isolate AMR_MDS_3026
            NODE_63008_length_2094_cov_2.973026, whole genome shotgun sequence.
ACCESSION   DAOTIL010000492 DAOTIL010000000
VERSION     DAOTIL010000492.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868050
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminobacteriaceae bacterium (wastewater metagenome)
  ORGANISM  Aminobacteriaceae bacterium
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae.
REFERENCE   1  (bases 1 to 2094)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2094)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:19:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,224
            CDSs (total)                      :: 3,198
            Genes (coding)                    :: 3,184
            CDSs (with protein)               :: 3,184
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2094
                     /organism="Aminobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_63008_length_2094_cov_2.973026"
                     /isolate="AMR_MDS_3026"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:3034609"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(216..>2094)
                     /locus_tag="P5201_09925"
     CDS             complement(216..>2094)
                     /locus_tag="P5201_09925"
                     /inference="COORDINATES: protein motif:HMM:NF024583.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="HD domain-containing phosphohydrolase"
                     /protein_id="HRV98899.1"
                     /translation="PYVDLITGELIVSVTAPVRSQSGMLLGVAGLDVSLEHVRRKVLT
                     RRLFGEGFPFLVDRHGFFLAAPFEDWTLAENIALRSVAIPAELAEIGEGILERESGAF
                     SLTFKGERMILFHAPVGEHFSFGMFFPESAMNSFVRDISSLHLFGGFAIILLALVLLL
                     PLTHEILSSFSSLSSTIDSIAAKLSDNKDFTETAFNVHLLAAEVGESMTRTSLLEFQT
                     FLGSMENALKTIGRQGEEIAALTEEALAIQDNLTEANGELNRRQKIWRDTLEVMETMT
                     MSGESEKNLRSVAENILKSTQAFGVLLSCRRGDRFFNLVCAGYGGASLPSGDAIAIFG
                     SVAGRAVREGDAVWVEDVARDREYVPVHPSVVSEVEIPLLHRGKANGILEVAFDRRTP
                     RNDDLLETLLPVASALGSLIEAENAHIEIKDSYRYLVRKLQSVTEIYHLESADHMDRI
                     GAYSRLAARILGKNEEEQEDIEVFSRLHDIGKLRVPLSILAKPGKLTDEEMKIVRNHP
                     LWGAELIGGAKWLDMARKICLSHHEKWDGSGYPLGLSGDEIPWEGQVVALADVYDALR
                     SRRVYKDRMAHEEAVRIILHGDGRTAPEHFSPLMLDFFREHHEELDKIYGAFFLGEAE
                     K"
BASE COUNT          474 a          614 c          524 g          482 t
ORIGIN      
        1 attttcatct ttagcaaaat aataaactta tgtttcaagg taggatgcaa aaatgggccg
       61 gttttgtcaa cccatagagc ttataaataa cgcattcctt aaataaaagt gtgggaaagt
      121 tcctgcacaa aagagcaggg actctcccac acgtattttt cccgttccgt gcattcagag
      181 aatgcgttga gatcactcct tccgcaatga ggcgtctact tctcagcctc cccaagaaag
      241 aatgctccgt agattttgtc caattcctca tgatgttctc tgaagaagtc gagcatcagg
      301 gggctgaagt gctccggcgc ggttcgtccg tcgccgtgca ggataatccg taccgcctcc
      361 tcgtgcgcca ttcggtcttt gtacacccgc ctggaacgca gcgcatcgta cacatccgca
      421 agcgccacga cctgcccctc ccaaggtatt tcgtctcccg aaaggccgag aggatagccg
      481 ctgccgtccc acttttcatg atgggagaga cagatcttcc tggccatatc aagccacttc
      541 gcgccgccga tcagctccgc cccccagagc ggatgattgc gaactatctt catctcttcg
      601 tcggtgagtt ttccgggttt tgccagaatc gagagcggca cgcgaagttt tcctatgtcg
      661 tgcagcctgg aaaacacctc aatatcctct tgctcttctt cgttcttgcc caaaatgcgc
      721 gccgcgagac gggagtacgc ccctattctg tccatgtggt ccgccgattc gaggtgatag
      781 atctccgtta ccgactgaag cttacggact aggtagcgat aggagtcttt tatctcgatg
      841 tgcgcattct cggcttctat caagcttcca agcgcggacg ccacggggag aagcgtttcg
      901 agcagatcgt cgtttctggg tgttctcctg tcgaaggcca cttcgagaat cccgttcgcc
      961 ttcccgcggt ggagcaaggg gatttcgact tcgctgacca cgctcggatg cacgggaaca
     1021 tactcgcggt cacgagccac atcttcgacc caaacggcat ccccctcacg cacagctctg
     1081 cccgcgacgc ttccgaagat tgctattgca tcgccggacg ggagcgaagc gccgccgtat
     1141 cccgcacaga cgaggttgaa aaaacgatcg cctcttcggc aggaaagaag caccccgaaa
     1201 gcctgcgtgc tcttcagtat gttctcggcg acgcttcgga ggtttttctc cgactcgccc
     1261 gacatggtca tcgtttccat gacctcgaga gtatctctcc atattttctg tctccgattc
     1321 agctctccgt tggcttctgt gaggttgtcc tgaatcgcaa gagcctcctc ggtaagcgcg
     1381 gcaatctctt cgccctgccg tccgatcgtc ttcagagcat tctccatact ccccaaaaat
     1441 gtttgaaact ccagaagcga cgtgcgcgtc atagattccc ccacttcagc agccagcaag
     1501 tggacgttga acgcggtttc ggtgaagtcc ttgttgtcgg agagtttggc ggcgatactg
     1561 tcgatcgtcg aggagagcga agaaaaagaa ctcaaaatct catgcgttag aggaagcagc
     1621 aagacaagag cgagcagaat gattgcgaac cctccgaaga gatgaaggga agatatatcc
     1681 ctgacaaagc tgttcatcgc gctctcgggg aagaacattc cgaacgaaaa atgctcgccg
     1741 accggggcgt gaaagagaat catcctctct cccttgaacg tcaggctgaa agccccggat
     1801 tccctctcca ggatgccttc gccgatttca gccaactccg cagggatcgc cacggagcga
     1861 agcgcgatgt tttccgcaag cgtccagtct tcgaagggag ctgcaaggaa aaaaccgtgt
     1921 ctgtcgacaa ggaatggaaa accttctccg aacagccttc tggtgagcac ttttcttcgc
     1981 acgtgctcca agctcacatc cagccccgcc acaccgagca acatccccga ctggcttcgt
     2041 acaggcgccg tgacgctcac gatgagctct ccggttatca ggtcgacata tgga
//
LOCUS       DAOTIL010000493         2091 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminobacteriaceae bacterium isolate AMR_MDS_3026
            NODE_63119_length_2091_cov_3.004912, whole genome shotgun sequence.
ACCESSION   DAOTIL010000493 DAOTIL010000000
VERSION     DAOTIL010000493.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868050
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminobacteriaceae bacterium (wastewater metagenome)
  ORGANISM  Aminobacteriaceae bacterium
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae.
REFERENCE   1  (bases 1 to 2091)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2091)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:19:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,224
            CDSs (total)                      :: 3,198
            Genes (coding)                    :: 3,184
            CDSs (with protein)               :: 3,184
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2091
                     /organism="Aminobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_63119_length_2091_cov_3.004912"
                     /isolate="AMR_MDS_3026"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:3034609"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..766
                     /locus_tag="P5201_09930"
     CDS             <1..766
                     /locus_tag="P5201_09930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005659581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter substrate
                     binding protein"
                     /protein_id="HRV98900.1"
                     /translation="VMNRTGGSGAVGMTEGATTKADGYTVTMITREIAWLPQMGLAQI
                     TPDNFDMIALVNEDPAIMLVQPDSKFTSVKDVIETAKAAPGTVKFGSTAKPNFYLLAL
                     ELNQNVKFNQIPYNGAAEAIPALLGGHVEFSMVNPGEAISQIMAGQLKPLGVCSDVRM
                     AGLPDVPTMTELGYPIVTGTWRGIAVPKGTPEDVKAILGAALEKAVAEKEFVDFMNGR
                     TLGIRFLNAADFKTFVENDVKALDAVVEEVKKQQQQ"
     gene            879..1346
                     /locus_tag="P5201_09935"
     CDS             879..1346
                     /locus_tag="P5201_09935"
                     /inference="COORDINATES: protein motif:HMM:NF018979.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter TctB
                     family protein"
                     /protein_id="HRV98901.1"
                     /translation="MKKANYGIVLVFLLLSAFVIYQANNFEQTLIQDDYVGASFFPEL
                     LAWMTAGLALFLGWLNFRGKMDDDGRKLADLFPRQILLAVVGLGLVVGYVMLLEPLGF
                     ILATIALNAALLLLFGVRSSLALALFPVIVSLSVYVVFYKILIVPLPEGLFYF"
     gene            1359..>2091
                     /locus_tag="P5201_09940"
     CDS             1359..>2091
                     /locus_tag="P5201_09940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005659584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter permease"
                     /protein_id="HRV98902.1"
                     /translation="MFTLLWGGLQTTFSLAIFPFLVLGVTGGIVIGALPGLTATMGVA
                     VLLPLTFGMESTRALVMLSGIYIGAIYGGSISAILLKTPGTPAAAATVLDGYELARKG
                     EAGKALSISAIASFTGGIVSTVMLITISPILARFALRFGAPEYFALAVFGLSIIASIS
                     GKSPVKGLLAGMFGLLISTVGLDPVTSFPRFTFGEINLFNGFAIIPVLIGLFALAEAF
                     VQMEKIFEPAQTAKTDFKRGMVSLKE"
BASE COUNT          383 a          660 c          601 g          447 t
ORIGIN      
        1 ggtgatgaac agaaccggcg gttccggcgc ggtcggcatg acggagggcg cgacgacgaa
       61 ggccgacggc tacaccgtca ccatgatcac ccgtgagatc gcgtggcttc cgcagatggg
      121 cctcgcccag atcaccccgg acaacttcga catgatcgcg ctggtgaacg aggatcccgc
      181 aattatgctc gtccagcccg attcgaagtt cacctcggtc aaggacgtca tcgagacggc
      241 gaaggcagct ccgggaacgg tgaagttcgg cagcaccgcg aagcccaact tctaccttct
      301 cgcccttgag ctgaaccaga acgtgaagtt caaccagatc ccctacaacg gcgcggcgga
      361 ggccattccc gcgcttctgg gcggccatgt ggagttctcc atggtcaacc ccggagaggc
      421 gatctcccag atcatggcgg ggcagctgaa gccccttggc gtctgctccg acgttcgcat
      481 ggccgggctt cccgacgtgc cgacgatgac cgagctcggg tatcctatcg tcaccggtac
      541 gtggagaggc atcgcggttc ccaagggaac gcccgaggac gtgaaggcaa ttcttggcgc
      601 ggccttggag aaggccgtcg ccgagaagga gtttgtcgac ttcatgaacg gccgcacgct
      661 cgggatccgt ttcctgaacg cagccgactt caaaaccttc gtggagaacg acgtgaaggc
      721 cctcgacgca gtcgttgaag aagtgaaaaa acagcagcag caataagaga acgcttcgtt
      781 ttcgttacag gacgtaacca cgcggggggc gccggttccg acggcggcct cccgcccttt
      841 cccaacgctc cacagataat tttggaggag aatgtcacgt gaaaaaagcc aactacggca
      901 tcgtacttgt ttttcttttg ctgagcgcat tcgtgatcta ccaggcaaat aacttcgaac
      961 agacccttat ccaggacgat tacgtcggcg catccttttt cccggaactg ctcgcctgga
     1021 tgaccgccgg acttgctctc ttcctggggt ggctcaactt ccgcggcaaa atggacgacg
     1081 acgggcgaaa gctcgccgac ctcttcccgc ggcagatact gcttgccgtt gtcggattgg
     1141 ggctcgtcgt cgggtacgtc atgcttctgg aacccctcgg tttcattctg gcaaccatcg
     1201 cgctgaacgc cgcgctgctt ctgctcttcg gcgtgcgcag ctctcttgcc cttgcactct
     1261 ttcccgtgat cgtctcgttg tcggtgtacg tggtgtttta taaaatcctc atcgtgcccc
     1321 ttccggaggg gctcttctac ttctaggagg cggcaggaat gtttactctt ctctggggcg
     1381 gtcttcagac caccttctct ctcgccatct tcccgttcct cgtcctcggc gttaccgggg
     1441 gcatcgtcat cggggcgctt cccggtctca ccgcgaccat gggcgtcgcg gtgctgcttc
     1501 cgctcacgtt cggcatggag tccacccgcg cgctggttat gctctcgggc atctacatcg
     1561 gcgccatcta cgggggctcc atctccgcga ttctgttgaa aactcccggg acgcccgccg
     1621 cagccgcgac agttctcgac ggctacgaac tggccaggaa gggcgaggcc ggcaaagcgc
     1681 tctcaatctc cgcaatcgct tcgttcaccg gcggtatcgt gagcacggtc atgctgatca
     1741 ccatctcgcc gatactggcc cgctttgcac ttcgcttcgg cgcgcccgag tacttcgcgc
     1801 tcgccgtctt cggcctgagc atcatcgcga gcatctccgg aaagagtccg gtgaaggggc
     1861 tcctggcggg aatgttcggc ctgctgattt cgacagtcgg gctcgacccg gtcaccagct
     1921 tcccgcgttt caccttcggc gagatcaacc tcttcaacgg cttcgccatc atccctgtgc
     1981 tcatcggtct cttcgcgctg gccgaggctt tcgtccagat ggagaagatc ttcgaacccg
     2041 cgcagactgc gaagacagat ttcaagagag gaatggtttc gctgaaggaa a
//
LOCUS       DAOTIL010000494         2090 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminobacteriaceae bacterium isolate AMR_MDS_3026
            NODE_63158_length_2090_cov_3.045700, whole genome shotgun sequence.
ACCESSION   DAOTIL010000494 DAOTIL010000000
VERSION     DAOTIL010000494.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868050
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminobacteriaceae bacterium (wastewater metagenome)
  ORGANISM  Aminobacteriaceae bacterium
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae.
REFERENCE   1  (bases 1 to 2090)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2090)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:19:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,224
            CDSs (total)                      :: 3,198
            Genes (coding)                    :: 3,184
            CDSs (with protein)               :: 3,184
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2090
                     /organism="Aminobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_63158_length_2090_cov_3.045700"
                     /isolate="AMR_MDS_3026"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:3034609"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..442
                     /locus_tag="P5201_09945"
     CDS             <1..442
                     /locus_tag="P5201_09945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012870169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HRV98903.1"
                     /translation="FLFALAAILGLHLFISRTRTGIAIRAGVSDMTMCSLMGVDLDRL
                     IAIVFLLAGAFAGVAGVFLGIKYLVYPTMGWVTNKAYIAAVIGGLGSLPGALAGGLIL
                     GVVETFVSTYVSSVLRDVFSFTLLIAFLVLRPNGLLGRDGEEKV"
     gene            445..1308
                     /locus_tag="P5201_09950"
     CDS             445..1308
                     /locus_tag="P5201_09950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006300358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HRV98904.1"
                     /translation="MDYILSIVILAGINMIAVLGLSIFTGFTGLFSLGHAAFVAVGAY
                     TSAILTYYYYVDYYLALAAGAAAAGIVSLIIGVPTLRAKLRSDYFAIAVLGFGEALRV
                     LLENLEITNGARGLPGIDNFTTLPIVFLCLAGGIFVARNFLRSRWGAACIAIREDPIA
                     AETAGVRLFRTRLLSLVVSAVYCGLSGGLLAHFLSFIQPQMFTLVQSTQLLAAVIAGG
                     MGSISGPLIASFVFIALPEALRVAQMWRLVAYGLLLVLIMVYRPQGLMGYSEVTSFFR
                     RKSGPEGGRRS"
     gene            1305..2090
                     /locus_tag="P5201_09955"
     CDS             1305..2090
                     /locus_tag="P5201_09955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013048810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HRV98905.1"
                     /translation="MSESKVLLSVAGLTKDFGGIRAVDSFSFEVREGSITGIIGPNGA
                     GKTTVFNLVTGLYKPTSGTIRFLGEEIGGNRPDVIVRHGIARTFQNIRLFNRRTCLEN
                     VLTPLLQRERCSFLGAMLGLPSVRRMERALHDKAHSLLSALGLDAYASFEASALPYGL
                     QRKLEIARALATEPSLLLLDEPAAGMNPEETHSLANLIAEVREKFNVTILLIEHHMDL
                     VMNICSPIVVMNFGALLATGTPEAVRANPEVVKAYLGERKEKR"
BASE COUNT          354 a          651 c          628 g          457 t
ORIGIN      
        1 gtttctcttc gcgcttgccg ctattcttgg actgcacctc ttcatcagcc ggactcggac
       61 gggcatcgcc atccgtgcgg gcgtctcgga catgacaatg tgctccctga tgggagtgga
      121 ccttgacagg ctcatcgcca tcgtctttct tctcgcggga gcattcgcgg gagtggcggg
      181 agtctttctc gggataaagt atcttgtcta cccgaccatg gggtgggtga ccaacaaagc
      241 gtacatcgcc gccgtgatcg gcggtctcgg cagcctcccc ggagcgcttg ccggagggct
      301 catcctcggc gtggtcgaga cgttcgtctc gacgtacgtc tccagcgtgc tgcgcgacgt
      361 cttcagcttc acgctgctga tcgcgttcct ggtgttgcgt cccaacggtc tcctcgggcg
      421 cgacggagag gagaaggtct agccatggac tatattctct ccatcgttat ccttgcgggg
      481 atcaatatga tcgccgtgct cgggctttcc atcttcaccg ggtttaccgg cctcttctcg
      541 ctggggcacg ccgccttcgt cgccgtcggc gcgtacacct ccgccatcct cacatactac
      601 tattacgtcg actactatct ggcgctcgct gcgggagctg cggccgccgg aatcgtcagc
      661 ctgatcatcg gcgtgccgac gctcagggcg aaacttcgaa gcgactactt cgccatcgcc
      721 gtgctcggct tcggcgaagc cctcagggtg cttctggaaa accttgagat cacgaacggc
      781 gcgcgcgggc ttccggggat cgacaacttc accacgctgc ccattgtttt tctctgtctt
      841 gccggaggga tcttcgtcgc ccgcaacttc cttcgctccc gctggggagc ggcgtgcatc
      901 gccatccgcg aggaccccat cgccgccgag acggcgggcg tcaggctctt cagaacccgc
      961 ctgctctccc tcgtcgtgag cgccgtctat tgcggtctct cgggagggtt gctcgcgcac
     1021 ttcctctcgt tcatccagcc ccagatgttc acgctggtgc agtcgacgca gcttcttgcg
     1081 gcggtgatcg ccggaggcat ggggagcatc tcggggccgc tgatcgcctc cttcgttttc
     1141 atcgcgctcc ccgaggcgct cagagtggcg cagatgtggc gtcttgttgc atacggcctg
     1201 ctccttgtgc tcatcatggt ctatcggccc cagggactga tggggtactc cgaggtcact
     1261 tcattcttcc ggaggaaatc cggcccggaa ggggggcggc gttcatgagc gaatcgaaag
     1321 tattgctctc tgttgcgggc ctgacaaagg actttggcgg aatacgcgcc gtggactcct
     1381 tctctttcga ggtccgagag ggctcgatca caggtatcat cggtccgaac ggcgcgggga
     1441 agacgacggt cttcaacctc gtcacaggat tgtacaaacc gacttcggga acgatccgtt
     1501 tcctaggaga ggagatcggc ggcaaccgtc cggatgtcat cgtccgccac gggatcgcaa
     1561 ggacgttcca gaatatacgt cttttcaacc ggcggacctg ccttgaaaac gtactcacgc
     1621 cgctgctcca gagggagcgg tgcagcttcc tcggcgcgat gctcggattg ccgtccgtcc
     1681 gcagaatgga acgcgcgctt cacgacaagg cgcattccct gctgtccgca ttaggactcg
     1741 acgcgtacgc ttctttcgag gcgagcgccc tcccgtacgg attgcagcgc aagcttgaaa
     1801 tcgcgcgcgc gctcgccacg gagccgtcgc ttctgctgct cgacgaaccc gcggccggta
     1861 tgaacccgga agagacgcac agtctcgcga accttatcgc ggaggtgcgc gagaagttca
     1921 atgtgacgat tcttctcatc gagcatcaca tggatctcgt gatgaacatc tgttcgccca
     1981 tcgtggtgat gaacttcggc gcgctgctgg cgacaggaac gcccgaagcg gtacgggcga
     2041 acccggaagt cgtcaaggct tatctgggtg aaaggaaaga aaaaagatga
//
LOCUS       DAOTIL010000495         2085 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminobacteriaceae bacterium isolate AMR_MDS_3026
            NODE_63339_length_2085_cov_4.274384, whole genome shotgun sequence.
ACCESSION   DAOTIL010000495 DAOTIL010000000
VERSION     DAOTIL010000495.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868050
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminobacteriaceae bacterium (wastewater metagenome)
  ORGANISM  Aminobacteriaceae bacterium
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae.
REFERENCE   1  (bases 1 to 2085)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2085)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:19:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,224
            CDSs (total)                      :: 3,198
            Genes (coding)                    :: 3,184
            CDSs (with protein)               :: 3,184
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2085
                     /organism="Aminobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_63339_length_2085_cov_4.274384"
                     /isolate="AMR_MDS_3026"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:3034609"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..407)
                     /locus_tag="P5201_09960"
     CDS             complement(<1..407)
                     /locus_tag="P5201_09960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006300338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="secondary thiamine-phosphate synthase enzyme
                     YjbQ"
                     /protein_id="HRV98906.1"
                     /translation="MKSHTEYLWFETKKRKELVHITPALQDILGRSGIGEGMMLVSAM
                     HITAGIIVNDNESGLHEDIMSWLERTAPEDPSYLHHRTGEDNGDAHLKRILIHHQAIL
                     PVTEGRLDLGPWERCFYAEFDGRRRKRVVVKVMG"
     gene            559..>2085
                     /locus_tag="P5201_09965"
     CDS             559..>2085
                     /locus_tag="P5201_09965"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HRV98907.1"
                     /translation="MTEFSNDTLFDDGAAATWRQDAGMLRAVLDVIPDIVSVQSPQLE
                     ILFYNKAGLDFLHASSEGAIGRNCYALLGRKEPCDDCLSLKAIRTGNAQEGEKYVPEL
                     DVWFEVRAIPVKDDAGSVRCVVEQLRDCTARKRNEERLARVLEELEAKNAELAETSAT
                     ARMLALRAEESSRAKSAFLANMSHEIRTPMNGILGMVELLLDTGLSMEQRDFGETISS
                     SADHLMDLLDDLLDFSKIEAERLTLESAPFNLQELVEDLTVQTAVKGREKNLEVYAVV
                     EPDVPAFFRGDASRLRQVLGNLLSNAVKFTDTGEISLAVSLAQDGILRTLLRFEVADT
                     GIGVPEEKKELLFKPFSQVDASITRRFGGTGLGLAISRGLVELMGGNIGMKDGQSGGA
                     LFWFEVPLDKIDHGDIEDLPVDPNLMNTPVLLVGGTSGWRRSVTTHLERWLCAVASAE
                     REGVEACLAGALDAERPFSVVLVDGSSVPEDNPFSPRALPERLRSVSSFYLLDYPSRR
                     W"
BASE COUNT          408 a          588 c          643 g          446 t
ORIGIN      
        1 cccatgacct tgacgaccac gcgcttacgg cggcggccgt cgaactcggc gtagaaacag
       61 cgctcccagg gaccgaggtc gagcctgccc tccgttaccg gaagaatcgc ctgatggtgg
      121 atcaagatgc gcttcagatg ggcgtcgccg ttgtcctccc ctgttctgtg atgaagatag
      181 gacggatctt ccggggctgt cctttccagc cagctcatga tatcctcgtg aaggccggat
      241 tcattgtcgt ttacaataat accggcggtg atgtgcatgg cggagacgag catcatccct
      301 tcgccgatac cgctccgccc cagaatgtcc tggagcgccg gggtgatgtg cacgagttcc
      361 ttgcgtttct ttgtctcgaa ccagaggtat tccgtatggc ttttcatact gatctctcct
      421 tcgtgatggt ggctgtacgt ggaatatggt agcatactct tttactgtcg acccgttttg
      481 tgctctatct tgaaaaggaa gaaattcgcc gctctccgaa gggacggcct gagcggagga
      541 ttgcgaggtg atgccgaaat gaccgaattt agcaatgata ctcttttcga cgacggcgcc
      601 gccgcaacgt ggcgtcaaga cgcgggcatg ctgagggccg tgctcgacgt catccccgat
      661 attgtttccg tacagagtcc gcagcttgag attctctttt acaacaaggc ggggctcgat
      721 ttcctgcatg cgtcttccga aggggcgatc ggccggaact gttatgcgct cctcgggaga
      781 aaagagccct gcgacgactg cctctcgctc aaggctatca gaacgggaaa tgcccaggag
      841 ggagagaaat acgttccgga gcttgatgtg tggttcgagg tgcgcgccat cccggtgaag
      901 gacgacgcag gtagtgtccg gtgcgttgtg gagcagctgc gcgactgcac ggcaagaaaa
      961 cggaacgagg agcgccttgc gcgcgttctc gaagagcttg aagcgaagaa cgcggaactt
     1021 gccgagacga gcgcaacggc ccgtatgctt gcgctccggg ccgaggagtc cagccgggcg
     1081 aagagcgctt ttctggcgaa catgagtcac gagatacgca cgcccatgaa cggaatcctc
     1141 gggatggtcg aactcctgct ggatacgggg ctctccatgg aacagcggga tttcggagag
     1201 acgatcagca gcagcgccga tcatctgatg gatctgctcg acgacctgct ggacttctcg
     1261 aagatagaag cggagcggtt gacgctggaa agcgccccgt tcaatcttca ggaactcgtc
     1321 gaggatctga cggtgcagac tgcggtgaaa ggacgggaga agaacctcga ggtctacgcc
     1381 gtcgtcgaac cggacgttcc cgcattcttc cgcggggacg cctcgcgtct tcggcaggtg
     1441 ctcggcaacc tgctctccaa cgccgtgaaa ttcaccgata ccggagaaat ctcgcttgcc
     1501 gtctcccttg ctcaagacgg tatcctcagg acgcttcttc gtttcgaggt tgcggatacc
     1561 ggcatcggcg ttccggagga gaagaaggag ctcctgttca aaccgttttc ccaggttgac
     1621 gcctccatca ccagacgctt cggcggaacc gggctcggac ttgccatttc gagggggctc
     1681 gtcgaactca tgggaggaaa catcggtatg aaggacgggc agtccggcgg cgctcttttt
     1741 tggtttgaag tccccctcga caaaatcgac cacggcgaca tcgaggatct ccctgttgat
     1801 ccgaatctga tgaatacccc cgtcctgctt gtcggaggaa cgtccggatg gaggcgctcc
     1861 gtaacgacgc acctggagcg ctggctctgc gccgttgcgt cggcggaacg tgaaggggtc
     1921 gaggcgtgtc ttgccggggc tctggacgcg gaacggcctt tttccgtcgt gcttgtcgac
     1981 gggagcagcg ttccggaaga caaccccttt tcccctcgcg cgcttccgga gaggctccgg
     2041 agcgtttcgt cattctacct gctcgactac ccttcaaggc ggtgg
//
LOCUS       DAOTIL010000496         2085 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminobacteriaceae bacterium isolate AMR_MDS_3026
            NODE_63341_length_2085_cov_4.019212, whole genome shotgun sequence.
ACCESSION   DAOTIL010000496 DAOTIL010000000
VERSION     DAOTIL010000496.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868050
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminobacteriaceae bacterium (wastewater metagenome)
  ORGANISM  Aminobacteriaceae bacterium
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae.
REFERENCE   1  (bases 1 to 2085)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2085)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:19:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,224
            CDSs (total)                      :: 3,198
            Genes (coding)                    :: 3,184
            CDSs (with protein)               :: 3,184
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2085
                     /organism="Aminobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_63341_length_2085_cov_4.019212"
                     /isolate="AMR_MDS_3026"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:3034609"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1128)
                     /locus_tag="P5201_09970"
     CDS             complement(<1..1128)
                     /locus_tag="P5201_09970"
                     /inference="COORDINATES: protein motif:HMM:NF013106.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase domain-containing protein"
                     /protein_id="HRV98908.1"
                     /translation="MKRPLRRVLCATLFALLFAAGGGLLWKASLPLPIDEVLEWPTSP
                     VLLDRRGEVFSVFLSPESEWSVPVSLEQMGRWLPFVAVEVEDRRFMEHAGIDYAALLR
                     AIWQNVSAGKIISGASTITSQLVRLSFPRPRTLKTKLVEFTAAQKLEEMLTKEEILEL
                     YLNRAPFGGNIRGVEAASRIYFSKGAGEVSLGEAALLVGMLKGPSLYRPDRNPGAARK
                     RRDAILASLFERGKITEEQYRLSRAERIPDTRGAFPSKAFHFALAALGERKKGGILET
                     TLDPALQELLERTLSVSLESLPEEITAAGIVMDNDSGDVLAYVGNGRLGRGIAGSWID
                     CARRPRSPGSALKPFAYLAAFEKGILTPASLLADTPLAFSGQ"
     gene            complement(1131..>2085)
                     /locus_tag="P5201_09975"
     CDS             complement(1131..>2085)
                     /locus_tag="P5201_09975"
                     /inference="COORDINATES: protein motif:HMM:NF036531.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRV98909.1"
                     /translation="PLGWMAHLNEHSDALRESGKIFLAGAYALASKTSEPLRKLGTGA
                     PSLRGGESTTFESASRNNALKLLMWSEVDPLSATASDLALKLIEEARSSTWRTTQDNG
                     MAVLALGRWMEKTRGARKPFTALVKDTSGATVASFTDGKRVSLSLKEVPQGPLTLSLD
                     GEGTAYFAWTSAGVPMKAPKPSSNGIALKRTWSGRDGKPLAEGAPLSRGDRITATLTM
                     TPSTALSDLVVVDMLPGGMEIENLRLTGEGTSPDQGGIRAELRDDRLILFVDFLTKPV
                     EYKYVIRAVSRGTFTLPPLAAEGMYAPDISAVGTPGTVEIN"
BASE COUNT          372 a          654 c          668 g          391 t
ORIGIN      
        1 ctgtccggag aaggcgagcg gagtgtccgc caggagcgac gcgggggtga gaattccttt
       61 ctcgaatgct gcaagatagg cgaacggctt gagcgcggag ccgggggagc gcgggcggcg
      121 ggcgcagtcg atccagcttc cggcgatccc tcttccaagg cgtccgttgc ccacataggc
      181 caaaacatcg cccgaatcgt tgtccatcac gattccggcg gcggtgatct cctcgggcag
      241 ggactccagg gagacggaca gcgtcctctc aagaagctcc tgaagcgcgg ggtcgagcgt
      301 cgtctccagt atgcctcctt tttttcgttc tccaagcgcg gcgagggcga aatgaaacgc
      361 cttcgacggg aaagcccccc gcgtatcggg aatccgttcg gctcgtgaaa ggcgatattg
      421 ctcttccgtg atctttcccc gttcgaaaag cgaggcgagt atggcgtcgc gccgttttct
      481 cgcggccccg gggttccggt cgggacggta gaggctcggc cccttgagca ttcccaccag
      541 gagcgccgcc tctccgaggg agacctcccc ggcgcccttc gagaagtaaa ttctggaggc
      601 ggcttcgacg ccccgtatgt tgccgccgaa cggagcgcgg ttcagataga gttccagtat
      661 ctcctccttc gtgagcatct cctcgagctt ctgcgcggcg gtgaattcca ccagcttcgt
      721 cttcagcgtc cgcgggcgcg gaaaagagag gcggacgagc tgacttgtga tcgtcgacgc
      781 tcccgaaata atcttgcctg ccgagacgtt ttgccatata gcgcggagga gcgcggcgta
      841 gtcgatcccg gcgtgttcca tgaaacgccg gtcctcaacc tcgacggcga cgaacggaag
      901 ccatcgcccc atctgttcga gcgatacggg aaccgaccat tcggattccg gagagagaaa
      961 aacggaaaag acctcccccc tgcggtcgag gagcacgggg gaggtcggcc attcaagcac
     1021 ttcgtcgatc ggaagcggga gggaagcctt ccagagaaga ccccctcctg ccgcgaaaag
     1081 cagagcgaac agcgtcgcgc agaggacgcg gcggaggggg cgtttcataa tcagttgatc
     1141 tccacggtcc ccggggttcc cacggccgag atgtccggcg cgtacattcc ctccgccgca
     1201 aggggcggca gcgtgaaggt tcccctggaa acggcgcgga tcacgtattt gtactcaaca
     1261 ggtttcgtca aaaagtcgac gaagaggatc agcctgtcgt cgcgcagttc cgcgcgaata
     1321 ccgccctgat ccggggacgt cccttcgccg gtgagccgga ggttctcgat ctccataccg
     1381 cccggcagca tatcgacgac caccagatcg ctcaacgctg ttgagggggt catcgtgagc
     1441 gtcgctgtga tccggtcgcc cctggaaaga ggcgcgcctt ccgcgagcgg cttcccgtca
     1501 cgcccgctcc atgtgcgttt gagggcgatt ccgttgctcg atggcttcgg cgccttcatc
     1561 ggtacgccgg ccgacgtcca cgcgaagtag gcggttccct cgccgtcgag cgagagcgtc
     1621 agcggtccct gcggaacctc cttcaggctc agagaaaccc gctttccgtc ggtgaacgaa
     1681 gcgaccgtcg cgccggaggt atccttcaca agcgcggtga acggtttgcg cgccccccgc
     1741 gtcttctcca tccaacggcc gagcgcgagc acggccatgc cgttgtcctg cgttgttctc
     1801 catgtgctgc tgcgggcctc ttcgatcagt ttgagggcca gatcggaggc tgtcgccgag
     1861 agaggatcga cctcgctcca catgaggagt ttcagcgcgt tgttcctcga cgctgactcg
     1921 aaggtcgtcg actcgccgcc ccggagcgaa ggggcgcctg tcccgagttt gcggagaggt
     1981 tccgatgttt tggaagccag tgcgtacgcg ccggcaagga agatctttcc ggactcgcgg
     2041 agcgcatcgc tatgttcgtt caggtgcgcc atccacccga ggggc
//
LOCUS       DAOTIL010000497         2085 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminobacteriaceae bacterium isolate AMR_MDS_3026
            NODE_63357_length_2085_cov_2.687685, whole genome shotgun sequence.
ACCESSION   DAOTIL010000497 DAOTIL010000000
VERSION     DAOTIL010000497.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868050
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminobacteriaceae bacterium (wastewater metagenome)
  ORGANISM  Aminobacteriaceae bacterium
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae.
REFERENCE   1  (bases 1 to 2085)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2085)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:19:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,224
            CDSs (total)                      :: 3,198
            Genes (coding)                    :: 3,184
            CDSs (with protein)               :: 3,184
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2085
                     /organism="Aminobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_63357_length_2085_cov_2.687685"
                     /isolate="AMR_MDS_3026"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:3034609"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..399)
                     /locus_tag="P5201_09980"
     CDS             complement(<1..399)
                     /locus_tag="P5201_09980"
                     /inference="COORDINATES: protein motif:HMM:NF016311.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase N-terminal domain-containing
                     protein"
                     /protein_id="HRV98910.1"
                     /translation="MDERRGVYSEETRRAFDGGPLWIHAVSVGEAQSAWPLLDALCPA
                     GGEKNLFPVLLSTTTTTGKEMAFRLADSLFERHIYYPWDIPRIVNRALDAIRPRGYAT
                     METEIWPNMLFSLARRGVPAFLVNGRFSERT"
     gene            complement(512..1567)
                     /locus_tag="P5201_09985"
     CDS             complement(512..1567)
                     /locus_tag="P5201_09985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013048873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="KpsF/GutQ family sugar-phosphate isomerase"
                     /protein_id="HRV98911.1"
                     /translation="MFCLPWEREGRCLSDVELLETGRGILHAEAAEIERAAQRTGLEL
                     VKAARIVHSCTGRLVVVGMGKSGIIGRKIAATLASLGTPSFFLHAAEGSHGDLGMVCR
                     DDAALFISNSGTTAEVVSLLPHFKRLGAPIIAITGGLNSPLAKNADAVLDSSVRSEAG
                     ITACATPLEPGERDQDALNLAPLCSTTLQLALGDALAGMVTELRGLRPEDFALFHPGG
                     ALGRRLLTRVGDVMGTGEKLPLVGMNVSVSDALFEMTSKGYGATVIVDEQGEIAGVFT
                     DGDLRRLIGSRGVECLSLEVGSVMTRNPLIIQPDKLAVEAVRVMERREISAIIVVEGK
                     RPVGILHLHELLQAGVA"
     gene            complement(1569..>2085)
                     /locus_tag="P5201_09990"
     CDS             complement(1569..>2085)
                     /locus_tag="P5201_09990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013048874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="3-deoxy-8-phosphooctulonate synthase"
                     /protein_id="HRV98912.1"
                     /translation="LLAAAGTGKPLNVKKAQFLAPGDMKHVVEKCRQGGCSGLMLCER
                     GTFMGYGQLVVDMRSLPLMRALGCPVVFDATHSVQMPGAAGGASGGDRRFVLPLARSA
                     VAAGIDALFLETHPRPEKAKSDGPNLVPLGKMRFLLEQLAELHAFVRERFGRLSLEWA
                     DEHAAENKGGR"
BASE COUNT          360 a          713 c          665 g          347 t
ORIGIN      
        1 ggtacgctcc gaaaagcggc cgttcacgag aaacgcaggg acgcctctgc gggcgagcga
       61 aaagagcatg ttcggccaga tctcggtctc catggtcgcg taccccctcg gcctgatcgc
      121 gtcgagggcg cggttcacga tgcgcgggat atcccacgga tagtagatat gccgctcgaa
      181 aagactgtcc gcgaggcgga aggccatctc cttgccggtc gtcgtcgtcg tggagaggag
      241 gacggggaag agattcttct cccctccggc ggggcacagc gcgtccagca gaggccatgc
      301 ggactgggcc tcccccaccg agactgcgtg aatccagagc gggcctccat cgaacgcacg
      361 gcgcgtctct tccgaataga cgcctctgcg ctcgtccagc ccctccgtat acttcctgag
      421 aagccacggg agcaggagag gataggctcc ggaggtacag actctgtaga acccttttcc
      481 cgctctccgg gtgagcgagt ccgtacccag ctcaggccac ccccgcctga agcagttcgt
      541 gaaggtgcag aatgccgacg gggcgcttcc cctccaccac gatgatcgcg gagatctccc
      601 gccgctccat cacgcgcacc gcttcgacgg ccagcttgtc cggctggatg atcagcggat
      661 ttctggtcat gacgctcccc acttccagcg agagacactc cacgcctctt gaaccgatga
      721 gccgccgcag gtcgccgtcg gtgaagacgc ccgcgatctc gccctgttcg tcgacgatga
      781 ccgtcgcgcc gtaccccttg ctcgtcatct cgaagagcgc gtcgctcacc gagacgttca
      841 tgccgacaag cggcagcttc tcccccgtcc ccatgacgtc gcccactctg gtgagcaacc
      901 tgcggcccag ggcccccccc ggatgaaaga gggcgaagtc ctccggccgg aggccccgaa
      961 gctccgtcac catccctgcg agcgcgtcgc cgagcgcgag ctgcaacgtc gtgctgcaca
     1021 agggggcgag gttgagcgcg tcctgatcgc gttctcccgg ttcgaggggc gtcgcgcacg
     1081 cggtgatgcc cgcctcggaa cgcaccgagg agtcgagcac ggcgtcggcg ttctttgcaa
     1141 gcggagagtt caaacctccg gtgatggcga tgatcggcgc gccgaggcgt ttgaagtgcg
     1201 ggagcagcga gacgacctcc gccgtggttc cgctgttgct gatgaagagc gccgcgtcgt
     1261 cgcggcagac catgccgagg tcgccgtgcg atccttcagc ggcgtggagg aagaaggagg
     1321 gcgttcccag ggaggcgagc gtcgccgcta tcttcctccc gatgatcccc gacttgccca
     1381 tgcccaccac cacgagcctg ccggtgcagg agtgtacgat acgcgcggcc ttcaccagct
     1441 ccaagcccgt tcgctgcgcg gccctttcta tctcggccgc ctcggcgtgc agaattcctc
     1501 tccccgtttc caggagttca acgtcggaga gacatcgtcc ctcgcgctcc cacggaaggc
     1561 agaacatctc agcgtccccc tttgttttcg gctgcgtgtt cgtcggccca ctccagggag
     1621 agacggccga atcgttcgcg gacgaaagcg tgcagttcgg cgagctgttc gaggagaaag
     1681 cgcatcttcc ccagggggac gaggttcgga ccgtcgctct tcgccttctc ggggcggggg
     1741 tgcgtctcca gaaagagcgc gtcgataccg gcagccacgg cgctccgggc aagggggagg
     1801 acaaaacgcc tgtcgccgcc gctcgcgccg cccgcggcgc cgggcatctg gacgctgtgc
     1861 gtggcgtcga agacgacagg acaaccaagc gcgcgcatga ggggcaggga gcgcatatcc
     1921 accacaagct gtccgtaccc catgaaggtg ccgcgctcgc agagcatcag gccggaacac
     1981 ccgccctggc ggcacttctc caccacgtgc ttcatgtcgc ccggcgccaa aaactgcgcc
     2041 ttcttgacgt tgagcggctt cccggttccg gcagcggcga gcagg
//
LOCUS       DAOTIL010000498         2084 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminobacteriaceae bacterium isolate AMR_MDS_3026
            NODE_63393_length_2084_cov_3.086249, whole genome shotgun sequence.
ACCESSION   DAOTIL010000498 DAOTIL010000000
VERSION     DAOTIL010000498.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868050
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminobacteriaceae bacterium (wastewater metagenome)
  ORGANISM  Aminobacteriaceae bacterium
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae.
REFERENCE   1  (bases 1 to 2084)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2084)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:19:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,224
            CDSs (total)                      :: 3,198
            Genes (coding)                    :: 3,184
            CDSs (with protein)               :: 3,184
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2084
                     /organism="Aminobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_63393_length_2084_cov_3.086249"
                     /isolate="AMR_MDS_3026"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:3034609"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..393
                     /locus_tag="P5201_09995"
     CDS             <1..393
                     /locus_tag="P5201_09995"
                     /inference="COORDINATES: protein motif:HMM:NF019349.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FCD domain-containing protein"
                     /protein_id="HRV98913.1"
                     /translation="ACEEFSSESMVALRANLQLQRLAISEKNASRFFALDEDFHRIIF
                     ATCGKERIWEAIRKMGSHFNRVRVLSLRATDWEKVFSQHERIASSIFEKKIGEARDAM
                     NEHLTKVNYGLGRLRQEYPSYFKTEKGR"
     gene            493..1476
                     /locus_tag="P5201_10000"
     CDS             493..1476
                     /locus_tag="P5201_10000"
                     /inference="COORDINATES: protein motif:HMM:NF015445.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter substrate-binding protein"
                     /protein_id="HRV98914.1"
                     /translation="MKKIALLIAVALLSSLVFSTAALAEKKPEFTLKLGHLANTEHTW
                     HKGSVKFAELVEEKTGGRVVVQVFPSEQLGNEMEVIGNIQSGIVDMLITGESMMNWAP
                     LCGLIAVPYMIRDSAHMEKIVEGEVGQAIEKEVIEKVRLRPVAWFERGARNLTSNREV
                     KKPEDLNGLRLRVPNVPLFVATWESLGAKPTPMAFSEVFTSLQQGIIDGQENPYDLIK
                     SASFFEVQKFVNKTEHVRGWIYLVIGEDKFQSMPEDIRKAVLEAGKEAQVYERQLFRA
                     EQDQLIKDLQDKGMTFVEVDKEAFMKKAEPAVLSFLSPELQEMYKKIVAIQ"
     gene            1555..2034
                     /locus_tag="P5201_10005"
     CDS             1555..2034
                     /locus_tag="P5201_10005"
                     /inference="COORDINATES: protein motif:HMM:NF016200.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter small permease"
                     /protein_id="HRV98915.1"
                     /translation="MKKPFNLFTRIMEFCVIVTFLGLVAVVLLQVYARLFLPRSPHWT
                     EEASRFLFAYTVSFAAGLAVKEKAYVNVDVFTNLLPEMLKQWLEVLLGLLVAAFMAFV
                     FYYSLKNLGIGRMRTSPSLTIPMHYIFASMSVMSGSIVLYSLAEVIRDTRALLKGGN"
BASE COUNT          456 a          567 c          571 g          490 t
ORIGIN      
        1 gcatgcgaag agttttcatc cgaaagcatg gtcgcgcttc gggcgaatct tcagctccaa
       61 cgcctggcta taagcgagaa gaacgcttca cggtttttcg ccctcgatga agatttccac
      121 cgcatcatct tcgcgacctg cgggaaggag cgcatctggg aggcgatccg gaagatgggg
      181 tctcatttca accgggttcg cgtgctcagt ctgcgggcga cggactggga aaaagtgttt
      241 tctcagcatg aacgcatcgc ctcctcgatt ttcgaaaaaa aaatcggtga agcgcgcgac
      301 gcgatgaatg aacatctcac gaaggtgaat tacggcctcg gaagactccg acaggagtac
      361 ccgtcctatt tcaagaccga aaaagggagg tgagcgccgg ccggcagcgt acgatttgtt
      421 tcgcggcgta aatcgccccc ggttcgttcg ggggaagacc tggtatcgta ttgatgaaag
      481 gagattgttc cgatgaaaaa aatcgcactc ttaatagcag ttgcgcttct gtcgtccctc
      541 gttttctcga ccgccgcact ggcggagaag aaacctgagt tcactttgaa actcggccac
      601 cttgccaaca ccgagcacac atggcacaag ggatccgtca agttcgccga acttgtcgag
      661 gaaaagaccg gcggccgcgt tgtcgtccag gttttcccca gcgagcagct tggaaacgaa
      721 atggaagtca tcgggaatat ccagtccggc atcgtcgaca tgctcattac cggagagtcc
      781 atgatgaact gggctcctct gtgcggactt atcgccgtcc cgtacatgat ccgcgactct
      841 gcgcacatgg agaagatcgt ggagggcgag gtaggccagg caatcgaaaa ggaagtcatc
      901 gagaaagtcc gccttcgtcc cgtggcgtgg ttcgagcgcg gcgcccggaa cctgacttca
      961 aaccgcgaag tgaaaaaacc cgaagacctg aacggcctga gacttcgtgt cccgaacgtt
     1021 cctctcttcg ttgccacctg ggagtctctc ggcgccaagc ccactcccat ggcgttctcc
     1081 gaagtgttca cctcgttgca gcaggggatc atcgacggtc aggagaaccc ctacgacctg
     1141 atcaagagcg ccagcttctt cgaggtccag aagtttgtca acaagacgga gcacgtccgc
     1201 ggatggatct atctcgtaat cggtgaggac aagttccaga gcatgcccga agatatccgg
     1261 aaagctgttc ttgaagcggg taaggaagcg caggtctatg agcgccagct tttccgcgcc
     1321 gagcaggatc agctgatcaa ggatcttcag gacaagggga tgacctttgt cgaagtcgac
     1381 aaggaagcct ttatgaagaa ggccgagcca gcagtgctca gcttcctctc tcccgaactt
     1441 caggagatgt acaagaagat cgtcgctatc cagtagcact atttcttccg ggccccggtc
     1501 ttccgggagc ccggattcct ttttcttttt ttgagagacg agggcgaccg tcatatgaaa
     1561 aaaccattca atctcttcac ccgtatcatg gagttctgtg taatcgtcac ttttctcggc
     1621 cttgtggccg tggtccttct ccaggtgtac gcccgcctct ttcttccccg ctctccccac
     1681 tggacggagg aggcttcccg ttttttgttc gcgtacacag tgagcttcgc tgcgggattg
     1741 gccgtaaagg aaaaagcgta tgtgaacgtg gatgtcttca ccaatttact tccggagatg
     1801 ttgaaacagt ggcttgaagt tttgctgggc cttcttgtcg cggcctttat ggctttcgtt
     1861 ttttactact cgctgaaaaa cctcggaata ggcaggatgc ggacgtcgcc ctccctgacc
     1921 atccccatgc actatatatt cgcctccatg tccgtcatgt cgggctccat agtgctctac
     1981 tcgctggcgg aagtcattcg ggatacgcgc gctctattga aggggggcaa ctgacgtggt
     2041 cgcgatgctt tttatcgtct ttctcattct tctctttgcg gggc
//
LOCUS       DAOTIL010000499         2080 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminobacteriaceae bacterium isolate AMR_MDS_3026
            NODE_63572_length_2080_cov_2.735309, whole genome shotgun sequence.
ACCESSION   DAOTIL010000499 DAOTIL010000000
VERSION     DAOTIL010000499.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868050
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminobacteriaceae bacterium (wastewater metagenome)
  ORGANISM  Aminobacteriaceae bacterium
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae.
REFERENCE   1  (bases 1 to 2080)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2080)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:19:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,224
            CDSs (total)                      :: 3,198
            Genes (coding)                    :: 3,184
            CDSs (with protein)               :: 3,184
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2080
                     /organism="Aminobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_63572_length_2080_cov_2.735309"
                     /isolate="AMR_MDS_3026"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:3034609"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..935
                     /locus_tag="P5201_10010"
     CDS             <1..935
                     /locus_tag="P5201_10010"
                     /inference="COORDINATES: protein motif:HMM:NF013703.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015297 - antiporter activity [Evidence
                     IEA];
                     GO_function: GO:0042910 - xenobiotic transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HRV98916.1"
                     /translation="FFAFSIVANNFVRAEGNASFAMMTMLISAGINTLLDPLFIFGFE
                     WGVRGAALATVAAQTATALWLAWYYLARKSAVRLRMKNLPPRAILLKETLSVGAAAFF
                     RQAASSLSLIVVNGSLAALGGGAAVAAYGIVNRLLLFAVMPVYGIVQGLLPIVGFNYG
                     AKQFCRVLSAMRRSIASATVLCTAGAAALFFFPGESVDFFTSSPEVRRAGIDAARILA
                     PGMLVTGFQIMASGLFQALGKAGPSFVLSLLRQVILLIPLVLLFSRLFGLFGVWLAFP
                     LSDFAASLLTFAIYRREMLRLREFCETGDDPTAS"
     gene            complement(922..>2080)
                     /locus_tag="P5201_10015"
     CDS             complement(922..>2080)
                     /locus_tag="P5201_10015"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="AGE family epimerase/isomerase"
                     /protein_id="HRV98917.1"
                     /translation="KRFGEENAWSMAESSIERIWEGGIHDHLGGGIARYATDRRWLLP
                     HFEKMLYDQALLLHVLSEYQNERGNPLFGRFADDLAGFVLREMTSPEGGFYSAFDADS
                     EGEEGKFYLWTEDQVRAVLTPEESGIFICAYGVRKGGNVKNERTGRILGDNVLHIPEP
                     RAKTAARFALSPDELDTLLASCRTRLFAERKRRIPPLLDDKILVDWNGLMIAALARAS
                     TVFKRPEWLTAAEKAARFIETKLRDKTGKLLHRYRDKEAAIPAFLDDYAFYAWGLTEL
                     AAATGKNAYTDAALKLADAMKENFLDKRNGGFFSSAGDDPMLFLRRKEAYDGAVPSGN
                     SVAMDALIRLSMISGRRDFASATKRTAEAFSERVAAFPLSHTRLLAAVMTL"
BASE COUNT          320 a          670 c          660 g          430 t
ORIGIN      
        1 catttttcgc gttctccatc gtggccaaca acttcgtccg ggccgagggg aacgcgtcgt
       61 tcgcaatgat gacgatgctt atttcggcgg ggatcaacac gctgctcgac ccgctgttca
      121 tcttcggctt cgagtggggg gtgcgcggcg cggccctggc cacggtcgcg gcgcagacgg
      181 cgacggcatt atggctggcg tggtactatc tcgcccgaaa aagcgcagtg aggctgcgga
      241 tgaagaatct ccccccgcgc gccatcctcc tgaaagagac gctctccgtg ggagctgcgg
      301 cgttctttcg ccaagcggcc tcaagcctct ccctcatcgt ggtgaacgga tcgcttgccg
      361 cgctgggagg gggcgccgcg gtggctgcct acggcatcgt gaaccggctg ctgctcttcg
      421 ccgtcatgcc tgtctacggc atcgtccagg ggctgctccc gatcgtgggg ttcaactacg
      481 gggcgaagca gttctgcagg gtgctctccg cgatgcgccg ctccattgct tccgccacgg
      541 ttctgtgcac agcgggcgcg gcagccctct tcttcttccc cggggagagc gtggacttct
      601 tcacgtcctc ccctgaggtt cggcgcgccg gcatcgacgc tgcgcgaata ctcgcgccgg
      661 gaatgctggt cacgggattt cagatcatgg cgtcggggct cttccaggcg ctgggaaagg
      721 ccgggccgtc gtttgttctc tccctgctgc ggcaggtgat cctcctgatc ccgctggtgc
      781 tgctcttctc ccgcctcttc gggctcttcg gcgtctggct cgcctttccg ctatcggatt
      841 ttgcggcgtc gctgctcacg ttcgccatct accgcaggga gatgctccgc ctccgcgaat
      901 tctgcgaaac gggcgacgac cctacagcgt catgactgcg gcaagcagcc gcgtatgcga
      961 aagcggaaac gcggcgaccc gctccgaaaa ggcttccgca gtcctcttgg ttgcggaggc
     1021 aaagtccctg cgtccggaaa tcatagacag ccggatcaac gcgtccatgg ccacggagtt
     1081 tcccgacggc acggcgccgt cgtaggcttc ctttcggcgc aggaagagca tcggatcgtc
     1141 ccccgccgac gagaaaaacc ctccgttccg tttgtccagg aaattttctt tcatcgcgtc
     1201 cgccagcttg agcgcggcgt ctgtgtacgc gttcttccct gtggctgcgg cgagttccgt
     1261 cagcccccag gcgtaaaatg cgtagtcgtc cagaaacgcc ggtattgcgg cctccttgtc
     1321 gcggtagcgg tgcagcagct ttcccgtttt gtctcggagt ttcgtctcga tgaaccgcgc
     1381 ggccttctcc gccgccgtca gccattcggg ccgtttgaag acggtggagg cgcgcgcgag
     1441 ggcggcgatc atgaggccgt tccagtcgac gaggatcttg tcgtcgagca gcggcgggat
     1501 gcgacgcttc cgttcggcga aaagccgcgt ccggcacgag gcgagcaggg tgtcgagctc
     1561 gtcgggtgaa agggcaaagc gcgcggccgt tttcgcgcgc ggttccggaa tgtgcaggac
     1621 gttatcgccg aggatgcgtc cggtgcgctc gttcttgacg ttgcccccct tccgcacgcc
     1681 gtaggcgcag atgaagatgc ccgactcctc gggggtcagg acggcccgca cctggtcttc
     1741 ggtccagagg tagaactttc cctcctcacc ttcgctgtcg gcgtcgaaag ctgagtaaaa
     1801 tcccccctcc ggagaggtca tctcacggag gacgaatcct gcgagatcgt cggcaaaccg
     1861 gccgaacaac ggattgcccc gctcgttctg atactccgaa agcacgtgca gcagaagcgc
     1921 ctggtcgtag agcatcttct cgaagtgcgg caacagccat cgccggtcgg tggcgtagcg
     1981 ggcgatgccg ccgccgaggt ggtcgtgaat gccgccctcc cagatacgct cgatggagct
     2041 ttcggccatg gaccatgcgt tctcttcgcc aaaacgtttc
//
LOCUS       DAOTIL010000500         2079 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminobacteriaceae bacterium isolate AMR_MDS_3026
            NODE_63592_length_2079_cov_4.494071, whole genome shotgun sequence.
ACCESSION   DAOTIL010000500 DAOTIL010000000
VERSION     DAOTIL010000500.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868050
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminobacteriaceae bacterium (wastewater metagenome)
  ORGANISM  Aminobacteriaceae bacterium
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae.
REFERENCE   1  (bases 1 to 2079)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2079)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:19:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,224
            CDSs (total)                      :: 3,198
            Genes (coding)                    :: 3,184
            CDSs (with protein)               :: 3,184
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2079
                     /organism="Aminobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_63592_length_2079_cov_4.494071"
                     /isolate="AMR_MDS_3026"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:3034609"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..238)
                     /locus_tag="P5201_10020"
     CDS             complement(<1..238)
                     /locus_tag="P5201_10020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006300440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar M-ring protein FliF"
                     /protein_id="HRV98918.1"
                     /translation="MNWFRQALSRGGLFWSSLRPWQRFSIVGASALVLLALILGVYWA
                     GRPTYEPLYSALEVEDQSAIVAWLRENKVDYKLDP"
     gene            complement(249..569)
                     /gene="fliE"
                     /locus_tag="P5201_10025"
     CDS             complement(249..569)
                     /gene="fliE"
                     /locus_tag="P5201_10025"
                     /inference="COORDINATES: protein motif:HMM:TIGR00205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-basal body complex protein FliE"
                     /protein_id="HRV98919.1"
                     /translation="MDGLKLDLMKMAGIQSLPAVAAVSEKKDTPRGTGLSFEDLLESS
                     MKQVNDLQQDSDKMVNRLATGDVDDISEVVLSAQRAEFALRLITEVRNKLVDAYQQLG
                     RMSV"
     gene            complement(746..1165)
                     /gene="flgC"
                     /locus_tag="P5201_10030"
     CDS             complement(746..1165)
                     /gene="flgC"
                     /locus_tag="P5201_10030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006583080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009425 - bacterial-type flagellum basal
                     body [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body rod protein FlgC"
                     /protein_id="HRV98920.1"
                     /translation="MRMFRSIDVSGSALTAHRLWMDSISSNLANVNTTRTPEGGPYKR
                     RVPVFAEMLDKTIGGYEDIGGVRVTEIAHDNTPPRLAYQPEHPDANAEGYVAFPNVNV
                     VREMTDMLVASRAYEANLAVADAARNMWAGALEIMRG"
     gene            complement(1168..1569)
                     /gene="flgB"
                     /locus_tag="P5201_10035"
     CDS             complement(1168..1569)
                     /gene="flgB"
                     /locus_tag="P5201_10035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012870082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009429 - bacterial-type flagellum basal
                     body, proximal rod [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body rod protein FlgB"
                     /protein_id="HRV98921.1"
                     /translation="MISDFNWKVMEKTMEGLSRRFESTSRNLANANTPEYARRNVSFE
                     DQLREVVDGPRSLPMTITNEGHIPSGPMAVSAVVPEEIKIYDEKFRLDGNNVDPEREM
                     AVLSQTRMAYNAMSRFVAKKGSLYRLVIGGR"
     gene            complement(1691..>2079)
                     /locus_tag="P5201_10040"
     CDS             complement(1691..>2079)
                     /locus_tag="P5201_10040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013048896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="GTP-sensing pleiotropic transcriptional
                     regulator CodY"
                     /protein_id="HRV98922.1"
                     /translation="FDPFSMRDLVLAEYLATLVGIEILHERTKNIEERARERLIVQMA
                     MRALSYSEVESVKHIITELGSFEGVVIASKVADRVGVTRSVIVNALRKLESAGIIESR
                     SLGMKGTFIKVLSPLFVEELGVLQGE"
BASE COUNT          423 a          614 c          576 g          466 t
ORIGIN      
        1 cagggtcgag tttgtagtct accttatttt cacggagcca tgcgacgatg gccgactggt
       61 cttcaacctc aagcgcggaa tagagcggtt cgtacgtggg acgtcccgcc cagtaaactc
      121 ccaaaatcaa ggcaagcaga acgagcgccg acgctccgac aatggagaat ctctgccacg
      181 gtcttaggga cgaccaaaaa aggccgcccc gcgagagcgc ttgccgaaac cagttcatcg
      241 gctacttcct acaccgacat gcgtccgagc tgctggtagg cgtcgacgag tttgttccgt
      301 acctcggtga tcaggcgcag ggcaaactcc gcacgctgcg ccgaaagcac tacttcagag
      361 atatcatcga catcacccgt ggccagcctg ttgaccatct tgtcggaatc ctgctgaaga
      421 tcgtttactt gcttcataga actctccagg aggtcttcga acgagagtcc ggttccccga
      481 ggcgtgtcct tcttttcgga aacggcagct acagccggca gggattggat ccccgccatt
      541 ttcattaaat cgagcttcaa gccgtccact tgaatctcct cctcttcgcg gtaagagtta
      601 ctggaacgta ttttaacaca acgggcaagg ttataacaaa aaagggaggc agtcgtgtgc
      661 gccccccgtt ccacaggtca tgagtcctga gactctttcg taaagaggag agagattccc
      721 cttttcggaa attcgaaaaa aaacatcaac cgcgcataat ctcaagcgcc ccggcccaca
      781 tgttccgggc agcgtccgcc actgcaagat tcgcttcgta cgccctgctg gcaacgagca
      841 tgtcggtcat ttctctgacg acgttcacgt tcgggaaggc gacatacccc tccgcattgg
      901 catcgggatg ctccggctga tacgcaagcc gggggggcgt gttgtcgtgc gcgatctcgg
      961 tcacgcggac gccgccgata tcctcgtagc cgccgatggt cttgtcgagc atctccgcga
     1021 agacaggtac ccgtcgcttg tacggtccgc cctcgggggt gcgcgtggtg ttcacgttgg
     1081 cgaggttcga cgaaatagag tccatccaca gacggtgcgc cgtgagcgcg cttccggaaa
     1141 catcgatgga acggaacatt ctcatggtca tcgtcctcct atgacaaggc gatagagcga
     1201 gccttttttt gcaacgaacc gcgacatggc gttgtaggcc attcgcgtct gtgacagtac
     1261 cgccatctct cgttcagggt ccacattgtt cccgtcgaga cggaattttt catcgtatat
     1321 ttttatctcc tcgggaacga ctgcgctgac cgccatcggt cccgagggta tgtgcccttc
     1381 gttcgtaatt gtcatcggca gactccgcgg accgtcgacg acctccctga gctgatcctc
     1441 gaaggagacg ttacggcggg cgtactcggg ggtgttggcg ttggcaaggt tgcgcgaagt
     1501 ggattcaaat cgtctcgaca gcccctccat cgttttttcc attaccttcc agttaaaatc
     1561 actgatcatc gccgtatccc tccctgaaaa cgctctttct cagtttcggc tctccgtgct
     1621 ccggacttga gcaaaagccg gcggttttcc ccgccggctc atatcatacc acagactttc
     1681 gtcttcggtg ttactctccc tggagcacgc ccagctcctc gacgaagagg gggctgagca
     1741 ctttgatgaa ggttcccttc atgccgaggc tgcggctttc gatgatcccg gcgctttcaa
     1801 gcttgcggag ggcgttgacg atgacgctgc gggtgacgcc cacgcggtcg gcgaccttgc
     1861 tcgcgatgac gacgccctcg aaggagccga gctcggtgat gatgtgtttg acggactcca
     1921 cttccgagta ggaaagcgcg cgcatggcca tctgcacgat caggcgctcg cgggcgcgct
     1981 cctcaatgtt cttcgtccgc tcgtggagaa tttcgatgcc cacaagcgtg gcaaggtatt
     2041 cggcaagcac gaggtcgcgc atggagaacg gatcgaaga
//
LOCUS       DAOTIL010000501         2079 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminobacteriaceae bacterium isolate AMR_MDS_3026
            NODE_63594_length_2079_cov_3.997530, whole genome shotgun sequence.
ACCESSION   DAOTIL010000501 DAOTIL010000000
VERSION     DAOTIL010000501.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868050
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminobacteriaceae bacterium (wastewater metagenome)
  ORGANISM  Aminobacteriaceae bacterium
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae.
REFERENCE   1  (bases 1 to 2079)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2079)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:19:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,224
            CDSs (total)                      :: 3,198
            Genes (coding)                    :: 3,184
            CDSs (with protein)               :: 3,184
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2079
                     /organism="Aminobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_63594_length_2079_cov_3.997530"
                     /isolate="AMR_MDS_3026"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:3034609"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(53..322)
                     /locus_tag="P5201_10045"
     CDS             complement(53..322)
                     /locus_tag="P5201_10045"
                     /inference="COORDINATES: protein motif:HMM:NF020396.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EsaB/YukD family protein"
                     /protein_id="HRV98923.1"
                     /translation="MAKVNVVIVDATGNKEQKVGLPDDIKCGIIMVKLVEKIKLPSVG
                     PDGNPISYKFIHKVTGRQLLESQTLAEAGIKDGDVLRLQPEITAG"
     gene            complement(447..1703)
                     /locus_tag="P5201_10050"
     CDS             complement(447..1703)
                     /locus_tag="P5201_10050"
                     /inference="COORDINATES: protein motif:HMM:NF033543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS256 family transposase"
                     /protein_id="HRV98924.1"
                     /translation="MSIVYRKNSGSVKGAIGLIDYELIEAKASEGLLRVSVEIGLEVV
                     RQMMETDVTELVGSKGKHNPGRTAYRHGWDESKVVLGSEKVSVRRPRVRGTDGTEMPI
                     KSLGLFQTDDPMNDAILSRLLSGVSTRKLCRTTDELVDDPACVSKSEVSRRFRSGMEK
                     AMAEFFGRRIEGSMPIMMLDGMGLGDMTIVAAMGIDEEGHKHMLGLTEGGTENSEVVS
                     ALLNDMILRGLDAQEPRLYVLDGGKALSKAVKDTFGKKAVVQRCQVHKKRNVLAHLPK
                     SEQENVSKRLTMAYREFDYEKANSSLELLAKEMDLRYPKASDSLREGLEETLTVHRLR
                     VPGLLRETLSSTNAMESANSVCAGVLRRVTNFKHGGTTLRHAAAGFMEAERSFHRVRG
                     YKQIPSLKMNLARLTETGDWSILGVA"
     gene            complement(1833..>2079)
                     /locus_tag="P5201_10055"
     CDS             complement(1833..>2079)
                     /locus_tag="P5201_10055"
                     /inference="COORDINATES: protein motif:HMM:NF025063.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="integrase core domain-containing protein"
                     /protein_id="HRV98925.1"
                     /translation="ARKSMGRVGSCYDNARMESFFATLKKEKLYRLDTLTMSVSDVRS
                     VIYRYIAYYNLRRIYSANGGYPPMVYRRMYYTGLLAA"
BASE COUNT          426 a          647 c          412 g          594 t
ORIGIN      
        1 tcttcctcaa aatcgcttga ctttaattcg atatcattcc ccatcattta cctcatccgg
       61 cagtgatttc aggctggagt ctgagaacgt ctccgtcctt gatacctgct tcagctaatg
      121 tctgcgattc aagaagctgg cgtcccgtca ctttgtggat aaatttatag ctgattggat
      181 ttccgtcagg ccctacagaa gggagcttga ttttctcaac cagcttaacc atgatgatcc
      241 cacacttgat gtcatcgggc aggccaacct tttgctcttt attaccagta gcgtcaacaa
      301 ttacaacatt tacctttgcc ataagtatca ttcctcactt tccattgcgg tattaataat
      361 tcggtagaac cttttctttg gagcctgtcc cagactccgt ggaatttgat aagaggcatg
      421 gcgacaccaa agcaacccgt ctcccatcaa gctacgccca gtatagacca atccccagtt
      481 tctgtcaatc gagccagatt cattttcagt gaaggaatct gcttatagcc cctcactctg
      541 tgaaagctgc gctcggcctc catgaacccg gctgcggcat gccgaagcgt cgtcccgcca
      601 tgcttgaaat tggttacgcg gcggagaaca ccggcgcaaa cggaattcgc cgattccatc
      661 gcgtttgtac tcgataacgt ctctcgcagt aagccgggga cgcgcaaccg atgcacggtc
      721 agcgtttcct ccagtccttc acgcaggcta tccgatgcct ttggataacg cagatccatc
      781 tctttcgcca gaagttcaag gctcgaatta gctttctcgt aatcaaattc ccgatatgcc
      841 atcgtcagtc gcttgcttac gttctcttgc tccgatttcg gtagatgcgc cagcacgttc
      901 cgtttcttgt gcacctgaca gcgctgtacc acggcctttt tcccaaacgt atctttgacg
      961 gctttgctca gcgctttccc gccgtcaaga acatatagcc gcggttcctg cgcgtccaaa
     1021 ccccgcaaaa tcatgtcgtt caacaacgca ctcaccacct cgctgttttc cgttccgcct
     1081 tccgtcaagc ccagcatatg cttgtgccct tcctcgtcga tccccatcgc agctacgatt
     1141 gtcatatctc caagccccat accgtccaac atcatgattg gcatgctgcc ctcgatccgc
     1201 ctcccgaaga actcagccat tgccttttcc atgccgcttc tgaatcgccg gctcacttcg
     1261 cttttgctga cgcaagcagg atcatccacg agttcatccg tcgttctgca cagttttcgc
     1321 gtgctcaccc cactgagcaa cctcgacagt atcgcgtcat tcattggatc gtctgtttga
     1381 aatagtccca aactcttgat cggcatttct gttccgtccg tcccgcgtac tcgcgggcgt
     1441 cttacagata ctttttcgct ccccaatacg accttgctct catcccagcc gtgccgatac
     1501 gccgttcgcc cgggattgtg tttccctttg ctgccaacca gttcggttac gtctgtctcc
     1561 atcatctgtc gaactacttc cagtcctatc tcgaccgata ctctcagcaa tccctcgctt
     1621 gcctttgctt cgatcagttc gtaatcaatt aacccaattg ctcccttgac gctcccggaa
     1681 ttcttgcggt atactatact catggtgaca attcctccct atctgttttc ttgtgttgtc
     1741 agctatagaa taacagattc tgacggtgtt gtcgcctttt ttcttcgttc ctccttcctc
     1801 aaattccacg ccgcttggga caagctcgag gcttacgccg ccagcaaacc cgtataatac
     1861 attctccgat acaccatcgg cggataacct ccgttcgcgg aatatatcct gcgcagattg
     1921 taatacgcaa tgtacctgta tatcacgctg cgtacatccg acacgctcat tgtcagcgtg
     1981 tcgagcctat acagtttctc tttcttcaga gtggcgaaga agctctccat ccgcgcgttg
     2041 tcatagcagc tgcctactct gcccatgctt tttctcgct
//
LOCUS       DAOTIL010000502         2075 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminobacteriaceae bacterium isolate AMR_MDS_3026
            NODE_63765_length_2075_cov_3.476733, whole genome shotgun sequence.
ACCESSION   DAOTIL010000502 DAOTIL010000000
VERSION     DAOTIL010000502.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868050
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminobacteriaceae bacterium (wastewater metagenome)
  ORGANISM  Aminobacteriaceae bacterium
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae.
REFERENCE   1  (bases 1 to 2075)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2075)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:19:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,224
            CDSs (total)                      :: 3,198
            Genes (coding)                    :: 3,184
            CDSs (with protein)               :: 3,184
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2075
                     /organism="Aminobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_63765_length_2075_cov_3.476733"
                     /isolate="AMR_MDS_3026"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:3034609"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..424
                     /locus_tag="P5201_10060"
     CDS             <1..424
                     /locus_tag="P5201_10060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011734096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HRV98926.1"
                     /translation="EVYTRAQMEEILLGKLIATSSKQIAMLLVILAVVSAAIVAFIIY
                     TLTLGKIREIAVLKLIGTKNRTIIGMIMQQAIALGVLGFMIGKVSATFWAPKFPKYVL
                     LEVRDAATGFAAVIIMCVIASLLAIRVAVKVDPAEAIG"
     gene            434..1153
                     /locus_tag="P5201_10065"
     CDS             434..1153
                     /locus_tag="P5201_10065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019677824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HRV98927.1"
                     /translation="MSGIRIEALRKRYGSGETAVDALKGVDMEVAPGEVVGLIGPSGS
                     GKSTLLKCLGAVIEPTGGKMVLGGKTIYEDGWKISDLRMLRRDSIGFIFQAPYLIPFL
                     NALDNVALLPMLAGHPDDESRERAMELLEALDVAHRAAAMPSQLSGGEQQRVSIARAL
                     ANHPPVILADEPTAPLDSERALSVVRILHSMAHQYETAIIVVTHDEKIIPTFRRIYRI
                     RDGKTVEEAGEGRSTAEEITE"
     gene            1169..1516
                     /locus_tag="P5201_10070"
     CDS             1169..1516
                     /locus_tag="P5201_10070"
                     /inference="COORDINATES: protein motif:HMM:NF019498.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HRV98928.1"
                     /translation="MIITRKYEEQKIAETAHRVDARGMYETEDAAISIIALQPGESLK
                     RHITPVDVAFFVLDGKGTVEIGDEKQEVGKYTLVESPKDILHCWYNTGDELLRFMVIK
                     APKPTKKTIFVGA"
     gene            1774..>2075
                     /locus_tag="P5201_10075"
     CDS             1774..>2075
                     /locus_tag="P5201_10075"
                     /inference="COORDINATES: protein motif:HMM:NF014912.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferritin family protein"
                     /protein_id="HRV98929.1"
                     /translation="MTFALSAADVFEAAILLEERGARFYADAAARCTGEAKKLLSGLS
                     SMEKNHAERFRSILEELRASSSAPEPRPEEAAQYLEALTSDRIFTETISIGAKESYP"
BASE COUNT          461 a          558 c          684 g          372 t
ORIGIN      
        1 cgaagtctac accagggcgc agatggaaga gattctgctg gggaaactga tcgccacgtc
       61 gtcgaagcag atcgctatgc ttcttgtgat actcgccgtt gtgagcgccg ccatcgtcgc
      121 cttcatcatc tatacgctca ccctggggaa gatcagggag atcgcggtac tcaaactcat
      181 cggcacgaag aaccggacga ttatcggcat gatcatgcag caggctatcg ccctcggcgt
      241 cctcggcttc atgatcggca aggtctcggc gaccttctgg gcgccgaagt tcccgaaata
      301 cgtgctgctg gaggtccgcg acgcggcgac gggattcgcc gccgtgatca taatgtgcgt
      361 gatcgcaagt cttctcgcga tacgggtcgc cgtcaaggtg gatcctgcgg aagcgatagg
      421 gtaggcggcc cctatgagcg ggatacgcat cgaagcgctc aggaagcggt acggctcggg
      481 agagacggcg gtggacgccc tcaagggagt ggacatggag gttgctcccg gagaggtcgt
      541 cgggctcatc gggccttcgg gatcggggaa gagtacgctg cttaagtgcc tcggcgccgt
      601 gatcgagccg acgggaggaa aaatggtgct cggcggcaag acgatctacg aggacggatg
      661 gaagatctcc gacctccgga tgctgcggcg ggacagtata gggtttatct ttcaggcgcc
      721 ctaccttatc ccttttctca atgcgctgga caacgtcgcg ctgctcccga tgctcgccgg
      781 tcatcccgac gacgaatcgc gggagcgggc catggagctt ctggaggcgc tcgacgtcgc
      841 gcaccgggcg gcggccatgc cgtcgcaact ttccggagga gagcagcagc gcgtctcgat
      901 cgcccgggcg ctggcgaatc atccgccggt catactggcc gacgagccca ccgcccctct
      961 ggacagcgag cgcgctctct ccgtggtccg gattctgcac tcgatggcgc accagtacga
     1021 gacggcgatc attgtagtga cgcacgacga gaagattatt ccgactttcc gcaggatcta
     1081 caggatccgc gacggaaaga cggtggaaga ggccggcgag ggccggtcga cggcagaaga
     1141 aataacagag tgaaggagga agaatactat gattatcaca aggaaatatg aagagcagaa
     1201 gatagcggag accgcgcaca gggtggacgc gcggggcatg tacgagaccg aggacgcggc
     1261 gatttcaatc atcgcgctgc aaccggggga gtcgttgaag cggcacatca ccccggtcga
     1321 cgtcgcgttt ttcgttctcg acgggaaggg cacggtcgag atcggcgacg agaagcagga
     1381 agtgggaaag tacacgctcg tcgaaagccc gaaggatatt ttgcactgct ggtacaacac
     1441 cggcgatgaa ctgctgcggt tcatggtgat caaagcgccg aaaccgacta aaaaaacaat
     1501 cttcgtcggc gcctgacgga gaggctgttc cggaagcgag cgcgccgcgt ccttggggaa
     1561 cggcgcgttc gctgtataat aaaagcagga acgggagttc caccgtttcc gatcaagatg
     1621 gaggcgtctt gacatattca ggaattaaag taccataata cctgtaaaga gtggagcgaa
     1681 agtgtgcaag cgtcttaaat accggcggtg tagtacggtg gtccgctgca cactccgccg
     1741 gagagagaac cggagacgaa aggggagatt gttgtgactt ttgcgctttc agcggcggat
     1801 gtcttcgagg ccgcgatcct gctggaggag agaggagcgc gcttctacgc cgatgcggct
     1861 gcacgctgta ccggcgaggc gaagaagctg ctctcggggt tgtcgtccat ggagaagaac
     1921 catgcggaac gcttccggag tatcctggag gagctccgcg cgtcgtcttc cgctccggag
     1981 ccccggccgg aagaggcagc acagtatctt gaggcgctga cctccgacag gatattcaca
     2041 gaaacgatca gcatcggggc aaaagagagc taccc
//
LOCUS       DAOTIL010000503         2074 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminobacteriaceae bacterium isolate AMR_MDS_3026
            NODE_63804_length_2074_cov_3.119861, whole genome shotgun sequence.
ACCESSION   DAOTIL010000503 DAOTIL010000000
VERSION     DAOTIL010000503.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868050
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminobacteriaceae bacterium (wastewater metagenome)
  ORGANISM  Aminobacteriaceae bacterium
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae.
REFERENCE   1  (bases 1 to 2074)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2074)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:19:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,224
            CDSs (total)                      :: 3,198
            Genes (coding)                    :: 3,184
            CDSs (with protein)               :: 3,184
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2074
                     /organism="Aminobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_63804_length_2074_cov_3.119861"
                     /isolate="AMR_MDS_3026"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:3034609"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..533)
                     /locus_tag="P5201_10080"
     CDS             complement(<1..533)
                     /locus_tag="P5201_10080"
                     /inference="COORDINATES: protein motif:HMM:NF024909.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HRV98930.1"
                     /translation="MLLKKNGVLLLVLAVCAAAVLGAGTVFAEQSETVVFEVTADTPV
                     VQTGEARKVVVRALVRPYTRRETVRAPLAVALVLDKSGSMASDGKMDNAKRGALEALK
                     MLDSRDVATIIVYDDNASVLVRARSVGNGAKEPAFLRAISRVRSGGMTALHDGVVLGA
                     EQLEPFIDEGFIPRIVLL"
     gene            complement(625..1380)
                     /locus_tag="P5201_10085"
     CDS             complement(625..1380)
                     /locus_tag="P5201_10085"
                     /inference="COORDINATES: protein motif:HMM:NF013077.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SagB/ThcOx family dehydrogenase"
                     /protein_id="HRV98931.1"
                     /translation="MMRMKKEEYRWFLKDTVRTTVDFSQTQQNLGYPPPPVQKPRPEG
                     SHIIPLPGPEEAGALCDISVAEAIRRRSSHRNYSAKPLAIEELSFLLWATQGVRGDRD
                     PVRSYRTVPSAGCRHPFETYLAVFRVESLQKGIYRYLPFDHALLPLGCPSDLESRMSR
                     AAHNQPFAGKSAVTFIWTALPERTEWRYSEASVKVIALDAGHVCQNLYLACEAADAGT
                     CAIAAYDQELADSLIGVDGENEFVLYMAPVGKL"
     gene            1731..>2074
                     /locus_tag="P5201_10090"
     CDS             1731..>2074
                     /locus_tag="P5201_10090"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRV98932.1"
                     /translation="MRKAGRILLLLLIAAIFIALSATAFMLAASTESASRVRLDWAHM
                     ANISAERVLSPADRSSPRAAMESFMNHMKEGYERLLKAKEISSTAPGLLHHPPEAMEE
                     AGRALYNFERAM"
BASE COUNT          470 a          640 c          530 g          434 t
ORIGIN      
        1 agcagcacga tacgggggat aaacccctcg tcgatgaagg gttcgagctg ctccgcacca
       61 aggacgacgc cgtcgtgcag cgctgtcatt cctccggagc gaacccggga aatcgccctg
      121 agaaaagccg gttctttcgc accgtttccg acggagcgag cccgtaccag aacggatgcg
      181 ttgtcgtcgt acacgattat tgtcgccaca tcccggctgt caagcatctt gagggcttcg
      241 agagctccac gtttcgcatt gtccatcttc ccgtcggacg ccatcgatcc tgatttgtcg
      301 agcaccagag cgacagcaag cggggcccga accgtctctc ttctggtata tggacgaaca
      361 agcgccctga caacaacctt ccgagcctcc ccggtctgca cgacgggggt atccgcggta
      421 acctcaaaga ccaccgtctc agactgttcc gcaaaaacag ttccagcgcc gagaaccgcc
      481 gcggcgcaca ccgccaaaac cagcaacagg actccattct ttttcaacaa catcttcacc
      541 acctcctggt ccgaaggata cagcgaaaaa accggcagaa tgtcatcgcc ccgtcaagcg
      601 gatgtcacaa aaatgtaaaa atcctcagag tttccctacc ggcgccatat agaggacaaa
      661 ctcgttctct ccgtcaacac cgatgagact gtcggcaagt tcctggtcgt aggcggcaat
      721 ggcgcacgtt ccggcgtctg cggcttcgca tgcaagatag aggttctggc agacatgccc
      781 tgcatcgagc gcgatcacct tcacggaggc ctcggaatag cgccattcgg tacgctccgg
      841 aagcgccgtc cagatgaacg tgactgcgct tttcccagcg aacggctgat tgtgcgcagc
      901 cctgctcatc ctcgattcaa ggtcggacgg acatccgagg ggaagcagcg cgtggtcaaa
      961 aggaaggtac ctgtagattc ccttctggag cgattccacc cgaaaaacag cgaggtacgt
     1021 ctcgaacgga tgacggcatc ccgcggaggg tacggtccgg taagagcgga cgggatcgcg
     1081 gtcgcctctg actccctgcg tcgcccacag caaaaagctc aactcttcaa tagccagagg
     1141 cttcgccgaa tagtttctat gggagctgcg gcgacggatg gcctccgcta cggaaatatc
     1201 acagagagct cccgcctcct ccggtccggg cagcggaatg atatggctgc cctccgggcg
     1261 cggtttttgc actggaggcg gcggataccc aagattctgc tgcgtctgcg aaaaatcgac
     1321 ggtcgtgcgt actgtatcct tcaaaaacca gcgatactct tcttttttca ttctcatcat
     1381 ttcattcctc cccggcaata tccggaatac atgcgaaggt cgcagttcct ggtgttcact
     1441 tcagttcttg gcgttcactt cactccttga ttatatactg cgacgctgta gcggcaaagc
     1501 cgaaacagta tcctcctgaa tccatcgacg ccgtcgaatg caccgtccga agttgcttga
     1561 gaatacattt tctgataaaa tgtattgcac acagcaaaaa ctctgcaatt tgtgcacacg
     1621 gcacacatct taaaataccc gccgtttcag cctttcttgt gcgaacgacg ttttttcatt
     1681 tcgtccacat ttcaggcata cgcagctcac gaaaggaagt ggttcaactc atgagaaaag
     1741 ccggccggat tcttctgctc cttctcatcg cggcgatttt catcgctctt tcagcaacgg
     1801 ccttcatgct tgccgcctcg acggagagcg cctccagggt tcgactcgac tgggcccata
     1861 tggcgaacat cagcgcggag cgcgtgctct ctcccgcaga cagatcgtct cctcgcgcgg
     1921 caatggaatc cttcatgaac catatgaaag aaggctacga acgactgctc aaggcgaagg
     1981 agatctcctc caccgccccg ggcctcctcc atcacccgcc cgaggcgatg gaggaggccg
     2041 gacgcgccct gtataacttc gaacgcgcca tgca
//
LOCUS       DAOTIL010000504         2072 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminobacteriaceae bacterium isolate AMR_MDS_3026
            NODE_63890_length_2072_cov_3.880516, whole genome shotgun sequence.
ACCESSION   DAOTIL010000504 DAOTIL010000000
VERSION     DAOTIL010000504.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868050
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminobacteriaceae bacterium (wastewater metagenome)
  ORGANISM  Aminobacteriaceae bacterium
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae.
REFERENCE   1  (bases 1 to 2072)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2072)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:19:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,224
            CDSs (total)                      :: 3,198
            Genes (coding)                    :: 3,184
            CDSs (with protein)               :: 3,184
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2072
                     /organism="Aminobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_63890_length_2072_cov_3.880516"
                     /isolate="AMR_MDS_3026"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:3034609"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..685
                     /locus_tag="P5201_10095"
     CDS             <1..685
                     /locus_tag="P5201_10095"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: protein motif:HMM:NF013146.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="MraY family glycosyltransferase"
                     /protein_id="HRV98933.1"
                     /translation="EAPLLRFMAVGSTLVFLSGYWDDMKSLNPAIRLTFHIVAAGLFL
                     LFLPVSLAHIPVCLLWITGMTNAFNLIDGANGLCLMMFIVASSVFAACGFPFFFLPVA
                     SLAAGVLLWNFPRAKTFLGDGGTTLLGYLYSSLCLYSLTPFLEDVHVFELPFFLLMAG
                     GIPAADTLFAIMRRLAMRKSPFSPDRGHIHHRLLDRGWSPVLTVMILSVAQLLVVAGG
                     VAFFGRFLV"
     gene            739..1875
                     /gene="wecB"
                     /locus_tag="P5201_10100"
     CDS             739..1875
                     /gene="wecB"
                     /locus_tag="P5201_10100"
                     /EC_number="5.1.3.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012869687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008761 - UDP-N-acetylglucosamine
                     2-epimerase activity [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine 2-epimerase
                     (non-hydrolyzing)"
                     /protein_id="HRV98934.1"
                     /translation="MEKKIVCVVGTRPEGIKMAPLIRVLRERNFPVTVLSTGQHLQML
                     HQSLEFFGIEADVNLSVMEERQTLDRVTAKVLGGVGAFLDRRPQDLVLVHGDTTTTMA
                     SALAAFYRSIPVGHVEAGLRSGDMARPFPEEANRIITDRLSSLWFAPTEQAAANLKNE
                     GLPVSEGTLAITGNTVIDALKLVLERKHDLCDSLRPLERFPGPLLLMTAHRRESWGEP
                     LESICGAIVDILRQNSEVRILIPLHKNPSVRDVIRRYLADEERAILCEPLDYPDFVWA
                     MQRSSVILSDSGGVQEESAELKKPLLVMRDVSERPEALETGTALLVGTNRVGIAETAL
                     RILSDDVFRKSLLSRGANPFGSGDASEKIADVIAKFFENRQGVA"
     gene            1931..>2072
                     /locus_tag="P5201_10105"
     CDS             1931..>2072
                     /locus_tag="P5201_10105"
                     /EC_number="2.5.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006583561.1"
                     /note="adds enolpyruvyl to UDP-N-acetylglucosamine as a
                     component of cell wall formation; gram-positive bacteria
                     have 2 copies of MurA which are active; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HRV98935.1"
                     /translation="MKIIGGTQLKGNITAQGAKNAALPVMAAAILLKGKKLKIDRVPN
                     LLD"
BASE COUNT          459 a          475 c          561 g          577 t
ORIGIN      
        1 ggaggctcct ttgcttcgtt tcatggcggt aggttctacc ctcgtgtttt taagtggtta
       61 ctgggacgat atgaaatctt tgaacccagc catacgcctt acgtttcata ttgttgccgc
      121 aggtcttttc ctgcttttcc tccctgtttc tttggctcat atacctgttt gtctgctctg
      181 gattacagga atgacgaacg ctttcaacct tatcgacgga gccaacggac tgtgccttat
      241 gatgtttatc gtggcatctt cggtttttgc agcgtgcggg ttcccctttt ttttccttcc
      301 agtcgcgagc ttggctgctg gagttcttct ctggaatttt cccagagcga aaaccttttt
      361 aggcgacggc ggaacaacgc ttctggggta tctctactca agcctttgtc tttattcctt
      421 gaccccgttt ctcgaagatg ttcatgtgtt tgaactgccc ttttttctgt tgatggcagg
      481 cggtattcct gcggcagata cgctctttgc tataatgaga cgattagcga tgagaaaatc
      541 tccgttttca cctgatcgcg gacacattca tcatcgtttg ctcgacagag gatggagccc
      601 cgtccttact gtaatgattc ttagtgttgc acagttgtta gtagtcgccg gcggggtcgc
      661 gtttttcggc cgttttctcg tgtaacagtg tccggagagt gtgcttttaa caggattttt
      721 gcgtagggat ggctgaggat ggaaaaaaaa atcgtatgtg tagtcgggac ccgtcccgaa
      781 gggataaaaa tggcgcctct gatccgtgtg cttcgggagc ggaacttccc ggtgacagtg
      841 ctttcgacgg ggcagcatct tcaaatgctg catcagtcat tggagttttt tggaattgag
      901 gccgatgtta atctttccgt gatggaggaa cgtcagacgc tcgacagggt gaccgccaaa
      961 gtgcttggtg gggtaggcgc ctttcttgat cgtcgaccac aagacctggt tttagtacat
     1021 ggcgatacca cgacaaccat ggcctctgct ctcgcggcct tttaccggag cattccggta
     1081 ggccatgtcg aggccggctt gcgaagcggc gatatggccc gtcctttccc ggaggaagcg
     1141 aacaggatta tcacagacag actgtcttca ttatggttcg ctccaacgga gcaagctgca
     1201 gcgaacctta agaatgaagg gcttcctgta tctgaaggga ctcttgcaat cacggggaat
     1261 acggttattg atgcgctgaa gctggtattg gaaagaaaac atgacctctg cgattctcta
     1321 cgtccgctag agcgtttccc gggacctctc cttctcatga ccgcgcatcg cagagagtca
     1381 tggggggagc ctcttgaatc aatatgtgga gctattgtgg atatactgag acaaaacagt
     1441 gaggtgcgga tacttatacc gcttcataaa aatccttcag tacgtgatgt tatcaggcgg
     1501 tatctagcgg atgaagagcg ggctattctt tgcgaaccac tcgactaccc tgattttgtc
     1561 tgggcgatgc aaagaagctc cgttattttg agcgacagcg gaggcgtcca ggaggaatca
     1621 gcggaattga aaaaacctct cctcgtgatg agggatgttt cggaacgacc ggaagctctc
     1681 gaaacaggta cggctcttct cgtcggaacc aatcgcgtcg ggatagcgga gacggcgtta
     1741 cgcattctct ccgacgatgt tttcaggaag agtcttttga gccggggggc gaaccctttt
     1801 ggaagcggcg acgcttctga gaaaatagct gatgtaatag cgaagttctt tgaaaacaga
     1861 cagggtgttg cctgagtctt tttgtgtttt tcacaatatt ttcgggagga attggaaagt
     1921 ggaatccaaa atgaaaatca tcggcggtac ccagctgaag gggaatataa ccgcacaggg
     1981 cgctaaaaat gccgcgcttc ctgtgatggc ggccgccata ctgttaaaag gcaaaaaatt
     2041 gaaaatcgat agggtcccca atcttctgga tg
//
LOCUS       DAOTIL010000505         2069 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminobacteriaceae bacterium isolate AMR_MDS_3026
            NODE_64017_length_2069_cov_4.160874, whole genome shotgun sequence.
ACCESSION   DAOTIL010000505 DAOTIL010000000
VERSION     DAOTIL010000505.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868050
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminobacteriaceae bacterium (wastewater metagenome)
  ORGANISM  Aminobacteriaceae bacterium
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae.
REFERENCE   1  (bases 1 to 2069)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2069)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:19:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,224
            CDSs (total)                      :: 3,198
            Genes (coding)                    :: 3,184
            CDSs (with protein)               :: 3,184
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2069
                     /organism="Aminobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_64017_length_2069_cov_4.160874"
                     /isolate="AMR_MDS_3026"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:3034609"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..756
                     /locus_tag="P5201_10110"
     CDS             <1..756
                     /locus_tag="P5201_10110"
                     /inference="COORDINATES: protein motif:HMM:NF039907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallopeptidase TldD-related protein"
                     /protein_id="HRV98936.1"
                     /translation="ESRFLASLQPRATALEAVRRTAMVLGGEPLPTGKYDLLLDPESS
                     ASFVEVLGELFLASNIHKNKSFLKGKLGEKIASPALTLVDDGTLKGGIGAAPFDGEGI
                     PCSRTCLLSGGVVESWLYNLKYARIDGVASTGNAARSVSGTPDVDCSNLFLLPGKWTP
                     QDLLLQVGSGIMVTELLGLHTMNPVSGDFSLGIKGVRIKDGTLSEPVAGMTVAGNLKD
                     VLRSIDLVGNDFKFFGSIGSCSLVIRDVTAAGT"
     gene            764..>2069
                     /locus_tag="P5201_10115"
     CDS             764..>2069
                     /locus_tag="P5201_10115"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: protein motif:HMM:NF013670.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008745 - N-acetylmuramoyl-L-alanine
                     amidase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HRV98937.1"
                     /translation="MLLLFLFAMFLPAEAESALQLFSGNQCLGNVNSLQQGAFSLVSA
                     TDMASLLELPAVAKDDTLVVTAGRNKIQLVSGASAAWLDVELVPLAASCVQRNGQWFL
                     ESGSALKLFHSLLSRSGKNASLRWGTEVSDGAPAPLVTPPEPTKTPSSPVRDAPAVSA
                     PSSARPLLKGVRWGIDDDKIRVVLDLEGTGEPEVQRGAGSVKVFFSSGPWPVPGIPSP
                     YREVNAGAMNFGDRLMIDFASSLVVHEILSLEAPKRLVIDFRRSAASGAPVVPPVVVH
                     APRPTPLPTLSPLPPSTVETPRSPSRTGKRIVVVDAGHGGKDPGAVANKIREKDLNLS
                     VSRMIVARLKEFGIDARLTRDSDIYLTLRRRTEIANTMNADLFISIHGNALPPGKSAT
                     GMEIYLMALPTDKDAMELARFENRELAEDNGDFIVTVNEVEQA"
BASE COUNT          379 a          619 c          613 g          458 t
ORIGIN      
        1 gaaagccggt ttcttgcgtc tctgcaaccc cgtgcgacag ctctggaggc tgtcaggcgt
       61 accgccatgg tgctcggcgg cgagccgctg cccacaggga agtacgatct gctccttgat
      121 cccgagtctt ccgcgtcatt cgtcgaagtg ctcggcgagc tctttctcgc ctccaacatt
      181 cataagaaca agtcgttcct gaagggcaag ctcggcgaaa agatcgcctc cccggcgctt
      241 accctcgtcg acgacggtac gctcaaagga ggaatcggcg ccgccccgtt cgacggagaa
      301 ggcattccgt gctctaggac ctgtctcctc tccgggggag ttgtcgaatc gtggctctac
      361 aacctgaagt atgcgcgaat cgatggggtt gcgtctacgg gaaacgccgc gcgctccgtc
      421 tccgggacgc ccgatgtgga ttgctcgaat ctcttccttc ttccgggaaa gtggactcct
      481 caggatcttt tgctccaggt ggggagcggt atcatggtga ccgagctgct cgggctgcat
      541 acgatgaatc ccgtgagcgg tgatttttcg cttgggatca aaggcgtgcg tatcaaggac
      601 ggaacgcttt cggaacctgt agcggggatg acggttgccg gaaacctcaa ggatgtactg
      661 cgctcaatcg atctcgtcgg caacgatttt aagtttttcg ggagcatcgg ctcctgttct
      721 ctggtgattc gtgatgttac ggctgccgga acgtaaagtc ctgttgcttc tcctgttcct
      781 tttcgccatg ttccttccgg cggaggcgga gagcgcgttg cagctctttt cgggcaatca
      841 gtgtctgggg aatgtcaact ccttgcagca gggcgcgttt tccctcgtct ccgcaacgga
      901 catggcatcg cttctggagt tgccggccgt tgcaaaggat gatacgctcg tcgtgacggc
      961 ggggagaaac aagatacagc tcgtatcggg cgcctcagca gcatggctgg acgtggagct
     1021 ggttccgctg gccgcttcgt gcgttcagcg aaacggtcag tggtttttgg aaagcggctc
     1081 ggcgctcaag ctttttcact ctcttctctc ccggtcgggg aaaaacgcct cgctgcgctg
     1141 gggaactgaa gtaagcgacg gagcgcccgc tcctctcgta acgccccccg agccgacgaa
     1201 aacaccctct tcccccgttc gcgacgcgcc tgccgtctcc gccccgtctt cagcccgtcc
     1261 tcttctgaaa ggggtccgct gggggattga cgacgacaag atcagggttg tactggacct
     1321 tgaagggacc ggcgaacctg aagttcagcg cggcgccggc agcgtgaagg tcttcttctc
     1381 ttcagggccc tggcccgttc ccggaattcc ttccccttac cgggaagtga acgcgggtgc
     1441 gatgaacttc ggcgaccgtc tgatgatcga cttcgcttcc tcgcttgtcg tccacgagat
     1501 cctctctctg gaagctccga agagacttgt catcgacttc agacgaagcg ccgcatcggg
     1561 agcgccggta gttcctccgg tcgttgtgca cgccccccgg cctactcctc tcccgacgct
     1621 ctctcccttg ccaccttcga ccgtggaaac gccccgctct ccttcccgga caggaaagcg
     1681 gattgtcgtc gtcgacgcag ggcatggggg aaaagacccc ggcgccgtag cgaataaaat
     1741 ccgggaaaag gatctcaatc tctcggtctc caggatgatt gtcgctcgtt tgaaggagtt
     1801 cgggatagac gcgcggctga cgcgcgactc ggatatctac cttaccctgc gccgccgcac
     1861 ggagattgcg aacactatga acgccgatct cttcatcagt atccacggaa atgcgcttcc
     1921 tccggggaaa agcgcaaccg gcatggagat ttatctgatg gcgcttccga cggacaagga
     1981 cgcgatggag cttgcccgct tcgaaaacag ggagttggcc gaggacaacg gcgatttcat
     2041 cgtcactgtc aatgaggttg aacaggccg
//
LOCUS       DAOTIL010000506         2068 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminobacteriaceae bacterium isolate AMR_MDS_3026
            NODE_64069_length_2068_cov_3.580229, whole genome shotgun sequence.
ACCESSION   DAOTIL010000506 DAOTIL010000000
VERSION     DAOTIL010000506.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868050
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminobacteriaceae bacterium (wastewater metagenome)
  ORGANISM  Aminobacteriaceae bacterium
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae.
REFERENCE   1  (bases 1 to 2068)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2068)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:19:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,224
            CDSs (total)                      :: 3,198
            Genes (coding)                    :: 3,184
            CDSs (with protein)               :: 3,184
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2068
                     /organism="Aminobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_64069_length_2068_cov_3.580229"
                     /isolate="AMR_MDS_3026"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:3034609"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..565
                     /locus_tag="P5201_10120"
     CDS             <1..565
                     /locus_tag="P5201_10120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006300408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HRV98938.1"
                     /translation="LLRLISREYLPTRGQITVGDRNLRKLRSSELPYYRREVGVVFQD
                     FKLLPNLTVFENVAFVLEAMGIPPKLVESRSNEVIDQVGLWRRRFLKPPQLSGGEQQR
                     VAIARAMANSPAVFLADEPTGNLDLRTSEEIMRLLLSINASGTTVIVATHDQYLVDAY
                     RQRLVELHNGRLKRDERRGRYFVDGEL"
     gene            552..1436
                     /locus_tag="P5201_10125"
     CDS             552..1436
                     /locus_tag="P5201_10125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013048838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="permease-like cell division protein FtsX"
                     /protein_id="HRV98939.1"
                     /translation="MASFRYAFRDTFRLLFRHWGLSLLTLLTAASVLYILGFASLFAM
                     NMRYMISRIEGALVVQAYMKRGETAEQALELVRSSPLVLSVRAISPEEALERLRAKLG
                     NQAQAVTLLGENPLPWSLEIQVQRAEHITPLVRDLMVLPSIEEVLYAGKLVERLATIS
                     RASSAVSGIILMLTLLISTLVIFNTIRIAIYSRKEEIEVMLLIGATSTYISLPFVIQG
                     MLLGAGGAILAIFGLWSTYSSAIEAIRSTLAFVDIIDNRRILAQFYVFLFAAGATTGW
                     MCSWLAVHRFTSQSMRSR"
     gene            1463..>2068
                     /locus_tag="P5201_10130"
     CDS             1463..>2068
                     /locus_tag="P5201_10130"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase M23"
                     /protein_id="HRV98940.1"
                     /translation="MKKTTVLSVLLLAAFISLFYSAPYCEAASDIDSKLVQEKARMRT
                     LERQISEHQQRVRQMGEREQGVISRIEELDQKRKMTEQRIRVLELRSAKTKQAVEDLR
                     REIQATEQSLVSMKRVLEDRLVNIYKYGGVAELNLLLSASTAHEAMETTLLLNRIALQ
                     DETMITDMLVKKERLGEAAAQLEAERNQLAINSAALEKSRKT"
BASE COUNT          418 a          579 c          614 g          457 t
ORIGIN      
        1 cctcctcagg ctcatctccc gggaatacct tcccaccagg ggccagatca ccgtcggaga
       61 cagaaatctg aggaagcttc gctcttccga gctcccgtac tatcggagag aggtcggcgt
      121 agtctttcag gatttcaaac tcctcccgaa cttgaccgtg ttcgagaatg tggcgttcgt
      181 actggaagcg atgggtattc cgccgaagct ggtcgagagc aggtccaacg aagtgatcga
      241 tcaggtggga ctctggagga ggcgtttttt aaagccgccc cagctctcgg gcggagaaca
      301 gcagcgggtg gcgatcgcgc gggcgatggc gaactcgccg gctgtattcc ttgccgacga
      361 gcctaccggc aaccttgatc tgcgaacctc ggaggagatc atgcgtctcc tgctctccat
      421 caacgcctcg gggaccacgg tgattgtcgc gacgcacgat cagtatctcg tggacgccta
      481 taggcagcgc ctcgtggagc tgcacaacgg acggctgaaa cgggacgagc gcagggggag
      541 gtatttcgtc gatggcgagc tttagatacg ctttcagaga cacattccgt cttctctttc
      601 gccactgggg tctgagcctt ctcacgttgc tcacagccgc ttccgtcctc tatattctcg
      661 gattcgcgtc gctcttcgct atgaacatgc ggtacatgat ctccaggatc gagggcgccc
      721 tcgtcgtcca ggcgtacatg aagcgaggag agacggcgga gcaggcgctc gagcttgtcc
      781 gttcgtcccc tctggttttg tccgttcgcg ccatctcccc ggaggaggcg ctcgaaagac
      841 tgcgtgcaaa gctgggcaat caggcgcagg cggtcacgct gcttggagag aatcctcttc
      901 cgtggagtct ggaaatccag gtgcagaggg cggagcacat cacgccgctt gtccgcgacc
      961 ttatggtgct tccgtcgatc gaggaggtgc tctacgccgg gaaacttgtg gagcgtctcg
     1021 ccacgatctc ccgggcatca agcgcggtat cgggaatcat tctcatgctg accctgctga
     1081 taagtacgct cgttattttc aatacaattc gcatcgccat ctactcccgg aaagaagaga
     1141 tcgaagtgat gcttctcata ggagcgacgt cgacctatat ctcgcttccg ttcgtcatcc
     1201 aggggatgct tctcggcgcg ggcggtgcga tattggctat cttcggtttg tggagcacct
     1261 attcttccgc cattgaagct atccgttcga cgctcgcgtt tgttgatata attgataacc
     1321 ggcgcattct ggcccagttt tacgttttcc ttttcgccgc cggagccact acgggctgga
     1381 tgtgcagctg gcttgccgtg catcgtttca catctcagag tatgcgttcc cggtagtcct
     1441 gtccgtgaag gaggttggcg gtatgaaaaa aacgacggtt ctctccgtac ttctgctcgc
     1501 agcgttcatt tccctgttct attcggcgcc gtattgcgag gctgcctccg atatcgactc
     1561 gaaactcgtt caggagaagg cgcggatgcg gacgctcgaa cggcagatat ccgaacatca
     1621 gcagcgtgtc cgccagatgg gggagcggga gcagggcgtt atctcccgga tagaggagct
     1681 tgatcagaag cggaagatga cggaacagcg cattcgcgtg ctcgaactga ggagcgcgaa
     1741 aacaaaacag gcggtcgagg atctgcgccg cgagattcag gcgactgaac agtctctggt
     1801 ctcgatgaag cgcgtgctcg aagatcgcct ggtgaatatc tataaatacg gcggcgttgc
     1861 cgagctcaat ctgctgctct cggcatccac ggcccacgaa gcaatggaga cgacgctgct
     1921 cttgaaccgc atcgctcttc aggacgaaac gatgatcacc gatatgctcg tgaaaaaaga
     1981 gcgtctcggc gaggcggccg cgcagcttga ggcggagcgg aatcagctcg cgatcaactc
     2041 cgctgctctc gaaaagagca ggaagact
//
LOCUS       DAOTIL010000507         2066 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminobacteriaceae bacterium isolate AMR_MDS_3026
            NODE_64170_length_2066_cov_3.381900, whole genome shotgun sequence.
ACCESSION   DAOTIL010000507 DAOTIL010000000
VERSION     DAOTIL010000507.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868050
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminobacteriaceae bacterium (wastewater metagenome)
  ORGANISM  Aminobacteriaceae bacterium
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae.
REFERENCE   1  (bases 1 to 2066)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2066)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:19:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,224
            CDSs (total)                      :: 3,198
            Genes (coding)                    :: 3,184
            CDSs (with protein)               :: 3,184
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2066
                     /organism="Aminobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_64170_length_2066_cov_3.381900"
                     /isolate="AMR_MDS_3026"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:3034609"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1643)
                     /gene="dnaX"
                     /locus_tag="P5201_10135"
     CDS             complement(<1..1643)
                     /gene="dnaX"
                     /locus_tag="P5201_10135"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:NF004046.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit gamma/tau"
                     /protein_id="HRV98941.1"
                     /translation="MSVSLYRRYRPRSFDEVAGQETVVEVLKKALQRDQVAHAWLFSG
                     PRGCGKTSAARLLAKALNCTQLKDGYEPCGECLQCVSIGAGDSLDVVEIDGASNNGVE
                     EIRELKTHVSLSPFSAKWKVYIIDEVHMLSISAFNALLKTLEEPPPFVVFILATTEPH
                     KVPVTIRSRCQHIPFHRIELRAICSRLEAVAEKEGVPADPRALREIARHADGALRDAL
                     SLMEQALSLGNGEVSMEAADRLLGGGTLSDLERWIASLKENNVQPFLFLEEMFQKGAS
                     PQRVVEGVFLLFRNLWAVKRWGRPVLEALSLAGSEMEFLCEEAASWKESQLSEMMLFC
                     SKLIPQVRAGLRSDVLSGLLAAKGLECRSFAEGPRANVKEEAPALRPEPPVVSPARPA
                     QRVEDVTEAGIRSAPPFELPSKPVALPSKTEASSRLPAMIAEPSPEESVPFEGGPSEA
                     GWRELEKRLFEKNLPLYCLLVGLSIAVEDNVLEIRFPEEAGYCFTVLSSERNCHILAS
                     EAKKLLGPQTELFLLWRGDRRPCSGNGGTKDDEDSPSWN"
     gene            complement(1801..>2066)
                     /locus_tag="P5201_10140"
     CDS             complement(1801..>2066)
                     /locus_tag="P5201_10140"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRV98942.1"
                     /translation="RSNRTDEGAALLRKAVETIDAFLQMETPPYEIQVLDLDGKGALR
                     IGPALVAREPLPGGTPPLAELRASLVQALGAALDKHRSANYHR"
BASE COUNT          418 a          625 c          632 g          391 t
ORIGIN      
        1 tcgttccagg acggcgagtc ttcatcatct ttcgttccgc cgtttcccga acacggacgg
       61 cggtcgcctc tccaaagaag aaagagttcc gtctgcggcc cgagcagctt cttcgcctcg
      121 gatgcgagga tatgacagtt tctctccgag gaaagcacag tgaagcagta cccggcctcc
      181 tccgggaaac gaatctccaa tacgttgtcc tccacagcga tcgacaagcc gacaagaagg
      241 cagtagagcg gcaggttttt ctcgaagaga cgcttctcca gctctctcca ccccgcctcg
      301 gacggtccgc cttcgaacgg aacggattct tccggggagg gctccgcaat catggcgggg
      361 aggcgcgacg acgcttcggt ttttgatgga agtgcaacgg gttttgatgg aagttcaaat
      421 ggaggcgcgc tgcgaatccc ggcttcagta acgtcctcga ccctttgcgc cgggcgggca
      481 ggagaaacaa cgggaggttc aggacgaagc gcaggcgcct cctctttcac gttcgcgcgg
      541 ggcccttccg caaagctgcg gcattcaagt cctttcgcgg ccagcaggcc ggagaggacg
      601 tccgagcgaa gccctgcgcg cacctgcggg atcagtttgg aacagaagag catcatctcg
      661 gagagctgcg attccttcca ggacgcagcc tcttcgcaca gaaactccat ctcgctcccg
      721 gcgagtgaaa gcgcctcaag aaccggcctg ccccatcgtt tcaccgccca gaggttgcgg
      781 aagagaagaa agacgccctc gacaactctc tgcggagatg ctcctttctg gaacatctcc
      841 tccaggaaga gaaacggttg gacattgttc tccttcaggg aggcgatcca ccgttcgagg
      901 tcggagagcg tccctccgcc gagcagacga tcggcggctt ccatggagac ctcgccgtta
      961 ccgagcgaga gcgcctgctc catcaaagag agcgcgtccc gcagggctcc gtcggcgtgc
     1021 cgcgcgatct cgcgcaacgc cctcggatcg gcaggaaccc cttctttctc cgcgaccgcc
     1081 tcaagcctgg agcagatggc ccgaagctct atcctgtgga aggggatgtg ctggcaccgg
     1141 gagcgtatgg tcaccggcac cttgtgcggc tccgtggtcg cgaggatgaa gacgacgaag
     1201 ggaggcggct cctcgagtgt tttcagcagc gcgttgaaag ccgagatgga gagcatgtgc
     1261 acttcgtcga tgatgtacac cttccatttt gcggaaaacg gagagaggga gacgtgcgtc
     1321 ttgagctcgc ggatctcctc aacgccgttg ttggaagctc cgtcgatctc gactacgtca
     1381 agactgtccc ccgcgcctat ggagacgcac tgtagacact cgccgcacgg ctcgtaaccg
     1441 tctttgagct gcgtacagtt aagagccttg gcaaggaggc gcgccgcgga ggtttttccg
     1501 cagccgcgcg gcccggagaa gagccaggcg tgggcaacct gatctcgttg aagcgccttc
     1561 ttcaacactt cgacgacggt ctcctggccg gcgacttcgt cgaacgaccg gggtctgtac
     1621 cggcggtaga gagatacgga cacggagtac tcccctttca aagggtcgag tcaaagggtc
     1681 gagcctgatc gaccgggact gcatgtcagt aaggagcaaa ctcctcctga tcgaaaaatg
     1741 cacaaaaaaa aggtgcgggg gccggcattt tctcccccgc acctattgta ccatcagaaa
     1801 tcagcgatgg tagtttgcgc ttcgatgttt gtccagggcg gctcccagag cctgcacaag
     1861 ggaggcgcgg agctcggcga gaggaggcgt tcctcccggg agaggttccc gggccaccag
     1921 ggcggggccg atacgaagcg cccctttgcc gtcgagatcg aggacctgta tctcgtaggg
     1981 cggcgtctcc atctggagaa aagcgtcgat cgtctcaacc gctttccgaa gaagcgcggc
     2041 cccttcgtct gtcctgttcg aacgcc
//
LOCUS       DAOTIL010000508         2066 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminobacteriaceae bacterium isolate AMR_MDS_3026
            NODE_64176_length_2066_cov_3.115863, whole genome shotgun sequence.
ACCESSION   DAOTIL010000508 DAOTIL010000000
VERSION     DAOTIL010000508.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868050
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminobacteriaceae bacterium (wastewater metagenome)
  ORGANISM  Aminobacteriaceae bacterium
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae.
REFERENCE   1  (bases 1 to 2066)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2066)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:19:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,224
            CDSs (total)                      :: 3,198
            Genes (coding)                    :: 3,184
            CDSs (with protein)               :: 3,184
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2066
                     /organism="Aminobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_64176_length_2066_cov_3.115863"
                     /isolate="AMR_MDS_3026"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:3034609"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1650)
                     /locus_tag="P5201_10145"
     CDS             complement(<1..1650)
                     /locus_tag="P5201_10145"
                     /inference="COORDINATES: protein motif:HMM:NF024509.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AsmA-like C-terminal domain-containing protein"
                     /protein_id="HRV98943.1"
                     /translation="MKKLFIALVVLVALAGLAVATMDFAGDLVVREAGPAVKEYLGAD
                     LAVAGIKGNPVKGFSMQGVSLKKDGQPLFSAKFLDVSLNLASLLSKSPKLSLLSVGGV
                     EIDADRLAAQVAQLKFEGGGGGEIPIETVRFVESVVVSKWARADITKLDLSFKGKEIL
                     ADVDMAVNKVPVKGSTTALLDGENVDIRSLAMNVGKGSVSASGKVAPSLAVSGNLKEL
                     DLKELISFWPVVSAEGFDGAVSLSFRGDGEWNAPALAGDMEYAGKSLLGYPVESVKAK
                     WTLGRDKLSVEGLDARVLGMPLAGGMSLGFAAGKAPVVDLSLSGSGIKLAELKKVYPD
                     IGDVSGEVDKFTINLAGTMETLSGVIEFSAPKLGLMGYSVSESRAQVKLTPETATVSA
                     KSQFEGAPITAQGSINEYMTAPKLNLTINVRSLNLTKIASSFPQLKELALQGGANADV
                     WVKGTAAAPEVTVKVWSEKISAMKEAIESPTVMLSLKGETITISNAAAKWRGAAIAAS
                     GTVEGSEKLNITATLENIQPGAIAPFYPDIAQLKIKGAVTAK"
     gene            complement(1750..>2066)
                     /locus_tag="P5201_10150"
     CDS             complement(1750..>2066)
                     /locus_tag="P5201_10150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008712045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="diaminopimelate epimerase"
                     /protein_id="HRV98944.1"
                     /translation="HTYALFPNGTNVNFVVPNDDTHIASVTYERGVEDLTDSCGTGST
                     ASAIAWVLRNAAVPSGTVVEVKNPGGVNFVTLSFDHSSEFVAASLKGRALMVAEGALF
                     EN"
BASE COUNT          417 a          656 c          534 g          459 t
ORIGIN      
        1 ttttgcagtc actgcgccct tgatttttaa ctgggcgatg tccgggtaga acggcgcgat
       61 ggcgccgggc tggatgtttt cgagcgtcgc ggtgatattc agtttctcgc tcccctcgac
      121 ggttcccgag gcggcgatcg cagcgccccg ccatttcgcc gcggcgttcg atatggtgat
      181 tgtctctccc ttgagcgaga gcatcaccgt cggcgattcg atcgcctctt tcatcgccga
      241 gatcttctcg ctccacacct tgactgtgac ttcaggagcc gcggcagtcc ccttaaccca
      301 cacgtccgcg ttcgcgcccc cttgaagggc cagctccttc aactgaggga aggacgatgc
      361 gattttcgtc agattcaagc ttctgacatt tattgtaaga ttgagcttcg gcgcggtcat
      421 gtattcgttg atcgacccct gagccgttat gggcgcgcct tcgaactgcg acttcgcgct
      481 cacggtcgcc gtctcgggag tcagcttcac ctgcgcgcgg gactcggaaa ccgagtaccc
      541 catgagcccc agtttcgggg cgctgaactc gatcacgccc gaaagcgtct ccatcgttcc
      601 ggcaaggttg atcgtgaatt tgtcgacctc gcccgagacg tctccaatgt ccggatagac
      661 cttcttcagt tcagcgagct ttatccccga tccggagagc gaaagatcga caaccggcgc
      721 tttcccggcg gcaaacccca gagacatgcc gcctgcgagc ggcataccga gcacccgtgc
      781 atccagcccc tcgacggaca gcttgtccct gccgagcgtc cacttcgcct tgacgctctc
      841 cacaggatac ccgagcagcg attttccggc gtactccata tcccccgcca atgccggggc
      901 attccactct ccgtctccgc ggaaggagag cgacacagcg ccgtcgaagc cttcggcgga
      961 aacgacgggc cagaaggaga tcagttcctt cagatcgagt tccttgagat tgccgctcac
     1021 cgcaagagag ggggctactt ttccggaagc cgatactgaa cccttcccga cgttcatcgc
     1081 cagcgagcgg atatcgacgt tttcaccgtc gagaagcgcg gtggtactcc ccttgacggg
     1141 aaccttgttg accgccatat caacgtcggc aagaatctcc tttcccttga aggaaaggtc
     1201 gagtttggtg atatctgctc tggcccactt ggagacgaca acgctctcga cgaaacgtac
     1261 tgtctctatc ggtatctcgc cgccgcctcc gccctcgaac ttcaactgcg caacctgggc
     1321 cgccagacgg tcggcatcga tctcgacgcc tccgacggag agcaggctca acttcggcga
     1381 cttcgagagc aacgacgcca agttgagcga gacatcaagg aatttcgcgc tgaagagagg
     1441 ctgaccgtcc ttcttcagcg atactccctg catcgagaaa cccttgacag ggttcccctt
     1501 gattcccgca accgcgaggt cggcgccgag atactccttt acggcgggac ctgcctcccg
     1561 taccacaaga tcgccggcga aatccattgt cgccactgcc agtccggcga gcgctacaag
     1621 aacaaccaac gcaatgaaca gcttcttcat ggttctctac ctccctttct tcgcgtcatt
     1681 gacgacgcgt cctctacatt cccccatccg aatcgtgata tgcttttctc attataacat
     1741 tctatctctt taattttcaa aaagagcgcc ctccgcaacc atgagggcgc gtcccttgag
     1801 agaagccgct acgaactcgc ttgagtgatc gaagctgagc gtcacgaaat tgactcctcc
     1861 cgggtttttt acctctacga ccgttccgga aggaacagcg gcgttccgaa gaacccacgc
     1921 aatagcggac gctgttgaac ctgtgccgca ggaatcggtg agatcctcca cacctctttc
     1981 gtaggttact gaagctatat gtgtgtcgtc attcggcacg acaaagttaa catttgtccc
     2041 gttgggaaaa agcgcgtagg tatgac
//
LOCUS       DAOTIL010000509         2064 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminobacteriaceae bacterium isolate AMR_MDS_3026
            NODE_64254_length_2064_cov_3.467397, whole genome shotgun sequence.
ACCESSION   DAOTIL010000509 DAOTIL010000000
VERSION     DAOTIL010000509.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868050
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminobacteriaceae bacterium (wastewater metagenome)
  ORGANISM  Aminobacteriaceae bacterium
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae.
REFERENCE   1  (bases 1 to 2064)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2064)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:19:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,224
            CDSs (total)                      :: 3,198
            Genes (coding)                    :: 3,184
            CDSs (with protein)               :: 3,184
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2064
                     /organism="Aminobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_64254_length_2064_cov_3.467397"
                     /isolate="AMR_MDS_3026"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:3034609"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..528)
                     /locus_tag="P5201_10155"
     CDS             complement(<1..528)
                     /locus_tag="P5201_10155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006300232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HRV98945.1"
                     /translation="MRRMHSGIFCVIFALVLLFAAASSAATPSGDPIKIGYLATLTGD
                     GATWGQHERDGALLAVKELNEAGGVLGRPLELVYYDVRGRQEDAIQAARRLIHEDKVV
                     AIGGTNYSGLNIAIASVVNSGEVPQIGTASTNPAVTVDPKTNTVRPYTFRLCYTDPYQ
                     GKVMADYLVNKLGVKS"
     gene            complement(690..>2064)
                     /locus_tag="P5201_10160"
     CDS             complement(690..>2064)
                     /locus_tag="P5201_10160"
                     /inference="COORDINATES: protein motif:HMM:NF016750.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HRV98946.1"
                     /translation="HSDCTFCGLCTQVCPVGALMEKRVERWPHLEEPTIVKTTCMLCP
                     VGCELDLNLDRKRSRIVRVTTDLDNPEAPTGGSCCVKGRYAFKDVADDGAFYPALKGA
                     CSSWSDAVSAFEELASGNSPAFVLGPSLTLEEVRALKEYTALRAPNAEMYAIWDAGFI
                     SLLNEAAKRKDVVRATYASMTGPSPSSDIRCGEADAFLLVGTNTDEDQPVLTSWLRRS
                     ARHRKSAVVYIGETPGLLDKGEAVVLHPEAGKEAQVLTALTSAVKAITAKESVASTNL
                     LSGTGVLPEDLEKAAGLLAGAKHPVTLIGAEASAKEVFDAAAALAGGRYMLLFKGSGS
                     VALISAIPGILPAATLRTVQKNMGALLFVGTTPEDAGFTADSLTERKYAVLSSVSSPL
                     AEKADVLLPLLPWIEKDGSITNLEGRTLSVRRGPLTKKTGKSLSALLAEAALPYEGRI
                     HANPVAR"
BASE COUNT          399 a          571 c          633 g          461 t
ORIGIN      
        1 gcttttgacg ccgagcttgt tgacgaggta gtcggccatg acctttccct ggtaggggtc
       61 ggtgtagcag agacggaacg tgtaggggcg cactgtgttt gtcttcggat cgacggtgac
      121 tgcggggttg gtgctcgcgg tacctatctg cgggacttcg ccgctgttca caacggaggc
      181 tatggcgata ttgaggccgc tgtagttcgt ccccccgatc gcaacgactt tgtcttcgtg
      241 aatcaaacgg cgggccgcct ggatagcgtc ctcctgtcgt ccgcggacgt cgtaatagac
      301 cagttcgagc ggccgtccaa gaacgccgcc cgcctcgttc agctctttga ccgcgagcag
      361 cgcgccgtcg cgttcatgct ggccccaagt ggcgccgtct ccggtgagcg tcgccagata
      421 cccgatcttg atcggatctc ccgacggtgt tgccgcagaa gaagcggcag cgaaaagcag
      481 taccagcgcg aagatgacgc aaaaaatgcc actgtgcatt cgtctcatga atttctcaac
      541 ctccctgata tgtgttaaaa aaatatttgg ttaattctaa aggccgttgc cctttgtgtc
      601 aatacggcta ataaaaagaa acaaaaattc acagggccgg aaggccctgt gaattttgat
      661 gacgctcgtt ctttttttgt gtttttgtat cagcgagcaa caggattggc gtgaattctt
      721 ccctcgtaag gcagagcggc ttccgcaaga agggcggaga ggctctttcc tgttttttta
      781 gtcagaggcc cccgtcggac cgagagcgtt cgtccttcga ggttcgtgat gcttccatcc
      841 ttttcaatcc atgggagaag cggtagcaga acgtcggcct tctcggcgag cggagaggat
      901 acggacgaaa ggaccgcata ctttctctct gtcaggctgt ccgcggtgaa gccggcatcc
      961 tcgggagtcg tccctacgaa gagcagggcg cccatgttct tctgaactgt gcgaagagtt
     1021 gccgcaggaa gtatcccggg tatggccgag atgagcgcaa cgcttcccga tcccttgaag
     1081 agaagcatat atcgtcctcc tgcgagcgcc gcagccgcgt cgaaaacttc ttttgcagac
     1141 gcctccgcgc cgatgagcgt cacgggatgt ttcgcaccgg caaggagtcc ggccgctttt
     1201 tcaaggtctt cgggaagaac gccggttcct gagaggagat tggtcgacgc gacgctttcc
     1261 ttggccgtta tcgccttgac tgcggatgtg agcgctgtga ggacctgcgc ttcttttccg
     1321 gcttccgggt gcaaaactac ggcttcgcct ttgtccagaa ggcctggagt ctcgccgatg
     1381 tagaccaccg ctgatttgcg gtgacgggca gacctgcgga gccaggatgt gagcaccggc
     1441 tggtcttcgt cggtgttcgt tcctacgagc aagaaggcgt cggcctctcc gcagcgtatg
     1501 tcgcttgacg gggaggggcc ggtcatggag gcgtaggtcg ccctgaccac atccttccgc
     1561 ttcgccgctt cattgagaag ggaaatgaac ccggcatccc aaatcgcgta catttccgcg
     1621 ttcggagcgc gaagggccgt gtactcctta agagcgcgaa cttcttcgag tgtgagcgat
     1681 ggaccgagta cgaacgcagg gctgttcccg gatgcgagtt cctcgaaagc cgagacggcg
     1741 tcgctccagg aggagcaagc tcccttgagc gcagggtaga aggcgccgtc gtcggcgacg
     1801 tctttgaagg cgtagcgccc ctttacgcag caggaaccgc ctgtcggcgc ctccggattg
     1861 tcgagatctg tggtaacgcg gacaatacgg gaacgcttcc tgtcgaggtt caagtcgagc
     1921 tcgcagccga ccgggcagag catacatgtt gtcttgacga ttgtaggctc ttccaagtgc
     1981 ggccagcgtt cgacgcgttt ttccataagc gccccgaccg ggcagacctg ggtgcagagg
     2041 ccgcagaaag tgcaatccga gtgt
//
LOCUS       DAOTIL010000510         2064 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminobacteriaceae bacterium isolate AMR_MDS_3026
            NODE_64258_length_2064_cov_3.161772, whole genome shotgun sequence.
ACCESSION   DAOTIL010000510 DAOTIL010000000
VERSION     DAOTIL010000510.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868050
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminobacteriaceae bacterium (wastewater metagenome)
  ORGANISM  Aminobacteriaceae bacterium
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae.
REFERENCE   1  (bases 1 to 2064)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2064)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:19:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,224
            CDSs (total)                      :: 3,198
            Genes (coding)                    :: 3,184
            CDSs (with protein)               :: 3,184
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2064
                     /organism="Aminobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_64258_length_2064_cov_3.161772"
                     /isolate="AMR_MDS_3026"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:3034609"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1898
                     /locus_tag="P5201_10165"
     CDS             <1..1898
                     /locus_tag="P5201_10165"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HRV98947.1"
                     /translation="FLEQWRGGGALAVSPFLLVHGRLSLPDSPAEAVETLLLSFGAFL
                     EGRAELPVYDHIAGVYRRELNDLDDVAPSTLSRREDVAGATGLRTPAERNRPVGAMPE
                     SVPYSAEDSIAFQAAEAPADFTSHDGAFLMDEETDAGYEPSPKEGAKMEKALRAASPA
                     PASPPAPLALFPPLPKKLAQPGGAERQMAESRITADAVLREEVFKKAEEEGFELLQRN
                     VAPVARTPSVLEAAKEPSKTVARGKRFEEVRAESLGGFLPRLSDDGIDLLFWSSRADG
                     TVAGFLVDVDVLKDRIDGVLPEILTDTRILTVLDERGEPLLAPETKELPDWRRPFVAR
                     EISPLLPRWEAGAWLADPTDAASRARFAARAVWLLVGALFFVILCGGALILWMLSSEM
                     RRARRKTTFVANVSHELKTPLTSIRLFAEMLLSGRQKDEERRKEYLRTMVSEAERLSR
                     LVENVLAFSRGGGGGESATEPLDLSELTRETLSQLEPNLLKNGFACSFSTGGPAPVRG
                     SPERLRQVLMNLLSNAEKYSGDVREIGIKCGNESGYAFVEVADRGPGVPSSQKEKIFQ
                     EFFRGDNSLTASRSGAGLGLSIARSIARRHGGDVSYMPRSGGGSVFSLRIPLEEAAAG
                     SGQGEEK"
BASE COUNT          370 a          584 c          678 g          432 t
ORIGIN      
        1 cctttctgga gcagtggaga gggggaggag cgcttgccgt ctctcccttt ttgcttgtgc
       61 acggacgtct ctctcttcct gattcgcctg ctgaggcagt cgaaacgctt ctcctttctt
      121 tcggggcgtt tcttgaagga cgagccgagc tgccggttta tgatcatatc gccggtgtat
      181 accgcaggga attgaacgat ctcgacgatg ttgccccctc caccctttcg cgcagggaag
      241 acgtcgcggg agcgacgggc ttgcggactc ccgcggaaag gaatcgcccc gtcggtgcga
      301 tgcctgaatc cgttccgtac tcggcggagg attccattgc attccaggct gcggaggcgc
      361 ctgcggattt tacttctcat gacggagcgt ttctcatgga cgaggaaacg gacgccggtt
      421 atgaaccctc tccgaaggag ggggcgaaga tggagaaagc acttcgcgcg gcttctcccg
      481 cacccgcctc tcctcccgcc cccctcgcgc tcttcccgcc gcttccgaag aagcttgcac
      541 agcctggcgg cgctgagcgg cagatggcgg agagccgtat tactgcggat gctgtgctgc
      601 gggaagaggt cttcaagaag gccgaggaag agggatttga gctgctgcaa cggaacgtcg
      661 ctcccgttgc ccgaacgcct tccgtgcttg aggccgccaa agaaccgtcg aagacggtgg
      721 ccagggggaa acgcttcgaa gaggtgcggg ccgaatcttt ggggggcttt cttccaaggc
      781 tttcggacga cggcattgat ctgctcttct ggagttcgcg tgccgacgga acggtcgccg
      841 gattccttgt ggatgttgat gtgttgaaag acagaatcga cggggtgctc cccgagatac
      901 tgaccgacac ccgtatactc accgttctgg acgaacgggg cgaacctctt ctcgccccgg
      961 aaaccaaaga gcttccggac tggcggcgcc cgttcgtcgc cagggagatt tcgccccttc
     1021 tgcctcgctg ggaggccgga gcgtggctcg ccgaccctac ggacgccgct tcccgcgccc
     1081 ggttcgccgc tcgggccgtc tggttgcttg tgggagcgct cttttttgtg atactctgcg
     1141 gtggggcgct tattctctgg atgctctcat cggagatgcg tcgtgcacgc cgaaagacaa
     1201 cctttgtcgc caacgtctcg catgagctga aaacgcctct cacttccatc cgcctcttcg
     1261 ccgagatgct gctctcgggg cggcagaagg acgaagaacg tcgaaaagag tatcttcgga
     1321 ctatggtctc cgaggcggaa cgtctttccc gtctggtaga gaacgtgctc gcgttttccc
     1381 gcggaggtgg cggcggagag tccgcgactg agccccttga tctttccgaa ttgacgcgcg
     1441 agaccctttc gcagctggag ccgaaccttt tgaaaaacgg ttttgcctgt tctttttcaa
     1501 ccggcggacc ggcgcctgtg cgcgggagtc cggagcgtct ccggcaggtg ctgatgaatc
     1561 tgctgtcgaa tgcggagaag tactccggcg atgtccgcga gatcggcata aagtgcggaa
     1621 acgagtccgg gtatgccttc gtggaagttg cggacagggg accgggggtt ccctcctcgc
     1681 agaaggaaaa gatctttcag gagttctttc ggggagacaa ttcgctgacg gcgtcgagaa
     1741 gcggggccgg actgggactt tcgatagcgc ggtcgatagc gcggcgccac ggaggcgacg
     1801 tgtcatatat gccaagaagc ggcgggggca gcgtcttttc gctgaggata ccgcttgagg
     1861 aagcggcggc aggctcggga caaggggagg agaaatgaac gaacgcattc tgatagcgga
     1921 ggacgacttc gccatcctga ccggggtggc ggatcttctg gagagcgaag ggtacgaagt
     1981 ggagaccgcc tgcgacgggc ttacggcgct cgaaagatac cgggccgtgc ggcccgacct
     2041 tgtgctcctg gatgtgatga tgcc
//
LOCUS       DAOTIL010000511         2062 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminobacteriaceae bacterium isolate AMR_MDS_3026
            NODE_64356_length_2062_cov_2.846039, whole genome shotgun sequence.
ACCESSION   DAOTIL010000511 DAOTIL010000000
VERSION     DAOTIL010000511.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868050
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminobacteriaceae bacterium (wastewater metagenome)
  ORGANISM  Aminobacteriaceae bacterium
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae.
REFERENCE   1  (bases 1 to 2062)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2062)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:19:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,224
            CDSs (total)                      :: 3,198
            Genes (coding)                    :: 3,184
            CDSs (with protein)               :: 3,184
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2062
                     /organism="Aminobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_64356_length_2062_cov_2.846039"
                     /isolate="AMR_MDS_3026"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:3034609"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(12..1340)
                     /gene="fliI"
                     /locus_tag="P5201_10170"
     CDS             complement(12..1340)
                     /gene="fliI"
                     /locus_tag="P5201_10170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009201689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009288 - bacterial-type flagellum
                     [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar protein export ATPase FliI"
                     /protein_id="HRV98948.1"
                     /translation="MTTKTADDELLQILEHRLGSVQLTRINGRIVQVVGLVAESQGPD
                     VRVGDLCSIRYRNSESSLSAEVVGFRGDRVLLMPLGNLKDVGPGCDVLSMERPLGVRV
                     GQALLGRVLDGLGNPLDDKGPIAASDFYPLYAEPPHPLRRKMITSPLPVGVKVIDGML
                     TLGTGQRIGIFAGSGVGKSTLLAMMARYTEADINVIALVGERGREVREFLDRDLGEEG
                     KKRSVLVIATSDQPPLIRLKAALTATAIAEYFRDCGKNVLLMMDSVTRVARAQREVGL
                     AVGEPPTTRGYTPSVFELLPRLLERAGAGEKGSITGVYTVLVEGDDMNEPVADTVRGV
                     LDGHVVLSRKIAARNFYPSVDLLESVSRVMPSIVDEEHLMAAGRVRELLAVYAEAEDL
                     ISIGAYKSGSNPRVDWAVTHLQKVRAFLRQSVGEHVPFDVMAQELKSLVP"
     gene            complement(1321..>2062)
                     /locus_tag="P5201_10175"
     CDS             complement(1321..>2062)
                     /locus_tag="P5201_10175"
                     /inference="COORDINATES: protein motif:HMM:NF014196.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="FliH/SctL family protein"
                     /protein_id="HRV98949.1"
                     /translation="KELQEEVQRLTSALRDAERENRDLAARVTLSEKELAKEKERLAA
                     ERREMEAAQKSEYESLRATAAAEGAEEGRKTGHAEGIRTAREEIAREYEQKVSSLVAL
                     LEKVHSTLSERTEELAALGMPRLIRLWELMLSKMLHREAQVSEGAVFPVLRSLLERLS
                     DRDRILVYLNPKDSEQTAERKDVFGDLLRGVKHLEFIPDANVEKGSCIVETNLGIYDA
                     RWQTQLEQIHREIEHLFLEGRKNDDENG"
BASE COUNT          357 a          627 c          635 g          443 t
ORIGIN      
        1 tcttcgcggt ctcacggcac gaggctcttc agctcctgcg ccatgacgtc gaaaggcaca
       61 tgttccccta ctgattggcg aagaaaggcg cggaccttct gcagatgcgt tacggcccag
      121 tcgaccctgg ggttgcttcc gctcttgtac gcgccgatgc tgatcaggtc ttccgcctcg
      181 gcgtagactg cgagcagctc gcggaccctc ccggcggcca tgaggtgttc ttcgtcgact
      241 attgagggca tgacgcggct gacgctctca agcagatcca ccgacggata gaagttcctc
      301 gcggcgattt tccgcgagag cacgacgtgt ccgtcaagaa cgccgcggac tgtgtcggcg
      361 acgggctcgt tcatgtcgtc tccctcaacg aggaccgtat agactcccgt gatgctccct
      421 ttctcgccgg cgcctgccct ttcgaggaga cgcggcagaa gctcgaagac tgacggcgtg
      481 tatccgcgtg ttgtcggggg ctcgccgacg gccaggccga cctcgcgctg cgccctcgcc
      541 acgcgggtga cggagtccat catcaagagg acgtttttgc cgcagtcgcg gaagtattcg
      601 gcgatcgccg tggcggtgag ggcggctttc agacggatga gcggaggctg gtcggacgtt
      661 gcgatgacaa ggacggagcg tttttttcct tcttctccga gatcgcggtc gagaaactcg
      721 cggacctccc tcccccgttc gccgacaagg gcgatgacgt tgatatcggc ctcggtgtag
      781 cgtgccatca tagcgagaag ggtgctcttt cccactccgg aaccggcgaa gatgccgatt
      841 cgctgacctg ttcccagtgt gagcatgccg tcgatgacct tgacgcctac aggaagcggg
      901 gacgttatca tcttgcggcg gagcgggtgg ggaggttcgg cgtagagagg gtagaagtcg
      961 gaggcggcga tgggcccctt gtcgtcaagc gggtttccga gtccgtcgag cacccgcccg
     1021 agaagggcct gcccgacgcg gactccgagt ggtcgttcca tggagaggac gtcgcacccc
     1081 gggccgacgt ccttgaggtt acccagaggc atcaaaagca ccctgtcccc cctgaagccg
     1141 acgacttcgg cggagagcga cgactcgctg ttcctgtacc gtatggagca gaggtcccct
     1201 acgcggacgt cggggccttg cgattcggcg acaaggccga caacctggac gatgcgcccg
     1261 ttgatgcggg tgagctgaac gctccccaac cggtgttcca gtatttgaag cagttcgtcg
     1321 tcagccgttt tcgtcgtcat ttttccgtcc ctcaaggaac agatgctcta tctctctgtg
     1381 aatctgctca agctgcgtct gccacctggc gtcgtagatt cccaggttcg tctcgacgat
     1441 gcagctcccc ttttccacat tggcgtcggg tatgaactca agatgcttga ctccccgaag
     1501 caaatcgccg aagacgtctt ttcgttcggc ggtctgctcg gagtcctttg gattcaggta
     1561 gacgagaatt cggtcgcggt cgctcaaccg ctcaagaaga ctgcgaagaa ccgggaaaac
     1621 agctccttcg gagacctgcg cctcgcgatg gagcatttta gagagcatga gctcccaaag
     1681 gcggataagg cgaggcattc caagggctgc aagctcttcc gtacgttcgg aaagcgtcga
     1741 gtgcactttt tcgaggaggg cgacgagcga ggaaactttt tgttcgtatt ctcgggcaat
     1801 ctcttcccgc gccgtccgga ttccttccgc atgtcctgtc tttcggccct cctcggcgcc
     1861 ttcggctgct gcagtggcgc gcagagactc atactcggac ttttgcgccg cctccatctc
     1921 ccgacgttcg gcggcaagac gctccttctc cttggcaagc tccttctcgg agagggtgac
     1981 gcgagcggca aggtcgcggt tttcccgttc ggcatcccgg agagcggacg taaggcgttg
     2041 tacctcttcc tgaagctcct tc
//
LOCUS       DAOTIL010000512         2052 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminobacteriaceae bacterium isolate AMR_MDS_3026
            NODE_64757_length_2052_cov_3.814221, whole genome shotgun sequence.
ACCESSION   DAOTIL010000512 DAOTIL010000000
VERSION     DAOTIL010000512.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868050
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminobacteriaceae bacterium (wastewater metagenome)
  ORGANISM  Aminobacteriaceae bacterium
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae.
REFERENCE   1  (bases 1 to 2052)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2052)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:19:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,224
            CDSs (total)                      :: 3,198
            Genes (coding)                    :: 3,184
            CDSs (with protein)               :: 3,184
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2052
                     /organism="Aminobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_64757_length_2052_cov_3.814221"
                     /isolate="AMR_MDS_3026"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:3034609"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            379..837
                     /locus_tag="P5201_10180"
     CDS             379..837
                     /locus_tag="P5201_10180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013047676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HRV98950.1"
                     /translation="MFKKILVAVDMSKMSEEVFAYGCSLALRLGAEATFLHVLPHPTL
                     WRGYEPWLPPEIDVEVAEIARKKLEYYLRKSKENYPELENMNYTMVVKEGNPSDVIIN
                     YAKEHDMHLVVIGYRGQSTLERLVVGSTASNVGRYAHCSVLIYRPGEQAI"
     gene            complement(984..1373)
                     /locus_tag="P5201_10185"
     CDS             complement(984..1373)
                     /locus_tag="P5201_10185"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRV98951.1"
                     /translation="MSLITCKLCGRLYDATRGKICSQCLDELDELYPRVREFIRDHSK
                     TSFSVDSLAEDMDVDILRMQSLVDLGYLDRDVPGISGADEEKKKKEELLQQLKGTLNT
                     SAPRERHSDEKTSKMYGQERYGTGKKK"
     gene            1607..2008
                     /gene="mce"
                     /locus_tag="P5201_10190"
     CDS             1607..2008
                     /gene="mce"
                     /locus_tag="P5201_10190"
                     /EC_number="5.1.99.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006584111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl-CoA epimerase"
                     /protein_id="HRV98952.1"
                     /translation="MKPTVVDHIGIAVKSIDEALAFWQSSLGIQCTGVEEVAEQKVKT
                     AFLPIGDTEVELLEATSDESPVAKFIEKKGEGIHHLAIRVDNLEKALEEMKAKGIKLI
                     DESPRYGAGGAKIAFVHPKSTGGILLELSER"
BASE COUNT          531 a          487 c          539 g          495 t
ORIGIN      
        1 tccgcaggag acgcacacgg atcgttctcc tccgctccag gattttccag agatgctccg
       61 taacgcgacg acgttcagaa acgagccgaa agatgcgccc aaaaaagaaa aaaaaacgat
      121 gagaagcgcg gtttccatat tcatcaatcc ttcacgaagg aattggctgc cagtttgatg
      181 cagaggcact ccttatctct atatatagca caaggagcgc attcagccaa actttctttc
      241 gcagaagagc ttcccccgca gatcgagcaa gagaatgaaa agaaagatgc gttctatatt
      301 ccggtttcag tattttgttg tataatattc tcaaagggcg accgattcat tcggtgaaat
      361 gtgaaaggag gaagagccat gttcaagaag attctggttg cggttgatat gagcaaaatg
      421 tcggaagaag tattcgcgta cggctgttcg ctcgctttgc gcctgggagc cgaggcgacc
      481 tttcttcacg tgcttcccca tcctacgctc tggagagggt acgaaccctg gctgcccccc
      541 gagatcgacg tggaggtagc ggaaatcgcc cgtaaaaagc tggaatacta cctccggaag
      601 tccaaggaga actatccgga gctggagaat atgaactaca caatggtcgt caaggaggga
      661 aatccctccg acgtgatcat caactatgca aaggaacacg acatgcacct ggttgtcata
      721 ggatacaggg gacagagcac tcttgagcgt cttgtggtcg ggagcactgc ctcgaacgtc
      781 ggacgatacg cgcactgttc ggtgctcatc tatcgcccgg gggagcaggc catctaagaa
      841 acgaactgtg atacgtgaag ccaagaaaaa ctcgacccgg gacagcggaa aacggttgtc
      901 ccgggttcgt catatctctc catggaatgc gttgaagcgg tggaaaaaag aactggggaa
      961 acctgttctt tccggcgctg tttttacttc tttttccctg tgccgtagcg ctcctgtccg
     1021 tacatcttcg atgttttttc gtctgagtgt ctctctctcg gagcggaggt attgagcgtg
     1081 cccttcagct gctgcaaaag ctcctctttt tttttcttct cttcgtctgc gccggatatc
     1141 ccgggaacgt cgcggtcgag gtagccgaga tccacaagcg actgcattct caggatgtcc
     1201 acatccatat cttcggcgag actatcgacg ctaaaactgg tttttgaatg atcccggatg
     1261 aactctctca ccctggggta taattcgtcg agttcatcaa ggcactgaga acagatcttt
     1321 ccgcgtgttg cgtcgtaaag cctcccgcaa agtttgcatg taatcaggct cacgatgacg
     1381 cctcccttct gcgtgttctt gacgaatact atatcactct ttagcggcag gaaaaagagg
     1441 gctgaggaaa aaagccaaag aacggagtgg tgaaattggc gtcgtctgac tacatgtata
     1501 caaatattct cttcttctga gagagtttct ccccatcttt tctgttaaga tatcctgtat
     1561 gcttctgttt catccggtaa aaagtatcga aaggagaaaa atactcatga aacctactgt
     1621 agtggatcat atcggaatcg cggtcaaatc aatcgacgag gcgcttgcat tctggcagtc
     1681 gagccttgga attcagtgta caggtgtgga agaagtcgcc gaacagaaag tgaaaacggc
     1741 atttctcccc atcggtgata cggaggtaga gcttctggag gctacctccg atgagagccc
     1801 tgtcgccaag tttatcgaaa agaaaggcga gggtatccac catcttgcta tccgggtgga
     1861 taatcttgaa aaggccctgg aggagatgaa agcgaaggga atcaagctga tagacgagtc
     1921 gccccgttat ggcgcgggag gggcgaaaat agctttcgtc cacccgaaga gcacgggcgg
     1981 tattcttttg gaactcagcg aaaggtagga ggttttttta tgtcgcagcg aacgatagaa
     2041 gagctctgcg ag
//
LOCUS       DAOTIL010000513         2046 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminobacteriaceae bacterium isolate AMR_MDS_3026
            NODE_65026_length_2046_cov_3.749874, whole genome shotgun sequence.
ACCESSION   DAOTIL010000513 DAOTIL010000000
VERSION     DAOTIL010000513.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868050
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminobacteriaceae bacterium (wastewater metagenome)
  ORGANISM  Aminobacteriaceae bacterium
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae.
REFERENCE   1  (bases 1 to 2046)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2046)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:19:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,224
            CDSs (total)                      :: 3,198
            Genes (coding)                    :: 3,184
            CDSs (with protein)               :: 3,184
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2046
                     /organism="Aminobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_65026_length_2046_cov_3.749874"
                     /isolate="AMR_MDS_3026"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:3034609"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..517)
                     /locus_tag="P5201_10195"
     CDS             complement(<1..517)
                     /locus_tag="P5201_10195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013047919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate--CoA ligase subunit alpha"
                     /protein_id="HRV98953.1"
                     /translation="MAVLVDGSTRVIVQGITGKSGILQTKSLLDYGTSVVAGVTPGKG
                     GAVVEGVPVFDSVLDAMKSVSPNAAISFVPPMFAKDAAIEAMEVGIKLLVLTMEGIPK
                     HDVLDILSYAQGRGVRVLGPGTAGVISPGKCKLGAHPARMFMEGKVGVVSKSGALSYE
                     VGKTLTDAGIGQ"
     gene            complement(519..1643)
                     /locus_tag="P5201_10200"
     CDS             complement(519..1643)
                     /locus_tag="P5201_10200"
                     /inference="COORDINATES: protein motif:HMM:NF020031.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRV98954.1"
                     /translation="MKLYEFQGKALFREAGIPVPKGAVVTASDREGLFAPSAVKAQVL
                     SGGRGKAGGVVLADTPDDAAKAADAILSMELKGEQVKALLVEEKANIVAEYYLAVTFD
                     GESRTPLFMASASGGMDIESVAEHAPEKILKLPVDPLWGLTDYKIRAMADFLGYEDRR
                     EFDGFVRKVWKLFREKDAVLVEINPLVVTDKGLLALDAKVEIDDDARPRRKQLFEAML
                     SEQRSLAGCEAEADHGTITYVPLEGDIGLISDGAGTGMLTLDLIRDMGGEAADFCEMG
                     GLTSPEVMYDAMKEVFSGKPGIKSLLVVLIGGFNRMDEMAEGIVRYLREHPVSIPLVV
                     RLCGTMEEEGKTIMKEAGLPVYDDLRTAVADAVRFAAGGE"
     gene            complement(1640..>2046)
                     /locus_tag="P5201_10205"
     CDS             complement(1640..>2046)
                     /locus_tag="P5201_10205"
                     /inference="COORDINATES: protein motif:HMM:NF013707.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016903 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="2-oxoacid:acceptor oxidoreductase family
                     protein"
                     /protein_id="HRV98955.1"
                     /translation="QDGIHNRQGRAIGTPTSDQNDIIVAMFQAAYDSHKHEIKKGGAF
                     FVDSGLVPEIGDVDPSVTVWRVPATETAIRLGSKMAGNMVMLGFLQEKTKVVSKESLM
                     ETIRGSVKEKFVAMNLAAFEEGVRLAREAAAP"
BASE COUNT          362 a          707 c          581 g          396 t
ORIGIN      
        1 actggcctat ccctgcgtcc gtgagcgtct tgccgacttc gtaggagaga gctccgctct
       61 tggagaccac gcccaccttc ccctccatga acatacgcgc agggtgcgcg cccagcttgc
      121 acttgcccgg cgagatgacg cccgccgtgc cggggccgag cacgcgcacg cccctgcctt
      181 gagcatagga aaggatgtcg agcacatcat gcttgggtat tccctccatc gtgagcacga
      241 gcagcttgat gcctacctcc atcgcctcga tcgccgcgtc cttcgcgaac atcggcggca
      301 cgaagctgat ggcggcgttc ggagaaacgc tcttcatcgc gtcgaggacg gagtcgaaaa
      361 caggtacgcc ctcgacgact gcgcccccct tgcccggcgt caccccggcg actaccgatg
      421 ttccgtagtc aagaagggac ttcgtctgca ggatccccga cttccctgta atcccctgga
      481 caatgacccg cgtgcttcca tcaaccagaa cggccatttc actcgcctcc cgcggcgaac
      541 cgcaccgcat ccgccacggc ggttcgaaga tcgtcgtaga cgggaagtcc cgcctctttc
      601 atgatggtct tcccctcttc ctccatggtg ccgcagagac gaaccacaag agggatggag
      661 acaggatgct ctctgagata gcgcacgatg ccctccgcca tctcgtccat gcggttgaaa
      721 ccgccgatga gcaccacgag caggctcttg ataccgggct tgccggagaa gacctccttc
      781 atggcgtcgt acatgacttc ggggctggtc aggccgccca tctcgcagaa gtctgccgcc
      841 tcgcctccca tgtcgcggat caggtcgagc gtgagcatgc ccgtcccggc gccgtcggag
      901 atgaggccga tatccccttc aagcgggacg tacgtgatgg tgccgtggtc ggcctccgct
      961 tcgcagccag caagagaccg ctgctcggag agcatcgcct cgaagagctg cttccggcgg
     1021 ggcctggcgt cgtcgtcgat ctccaccttc gcgtcgagcg caaggagtcc cttatcggtg
     1081 acgacgagcg gattgatctc cacaaggacg gcgtccttct cgcggaagag cttccagacc
     1141 ttccggacga atccgtcgaa ctccctgcgg tcttcgtacc cgaggaaatc cgccatcgca
     1201 cggattttgt agtcggtcag cccccagaga ggatcgacgg gaagcttcag aatcttctcg
     1261 ggcgcatgct ccgcgacgga ctcgatgtcc atcccgcccg aagcgctcgc catgaagagc
     1321 ggggtcctcg actctccgtc gaaggtgacg gcgaggtagt actccgctac gatattcgcc
     1381 ttctcctcga cgaggagcgc cttcacctgc tcccccttca gctccatgga gagaatggcg
     1441 tcggccgcct tcgccgcgtc gtcgggcgtg tccgcaagga cgacgccccc cgccttccct
     1501 cttcccccgg agagcacctg cgccttcacg gccgacgggg cgaaaagccc ttcccggtcg
     1561 gaggctgtga cgaccgctcc tttgggcacg gggataccgg cctcccggaa gagcgccttc
     1621 ccctggaatt cgtagagttt catggggcag ccgcctcccg cgcgagcctg acgccctctt
     1681 caaaggccgc aaggttcatg gcgacgaact tctccttcac cgacccgcgg attgtctcca
     1741 tcaggctctc cttcgaaaca accttcgtct tctcctggag gaagccgagc atgaccatat
     1801 ttcccgccat cttgctcccg agacggatcg cagtttcagt cgcgggtacc ctccagaccg
     1861 tgacggaggg atcgacatcg ccaatctccg gcacaaggcc gctgtcgacg aaaaaggccc
     1921 cgcccttttt aatctcgtgc ttgtggctgt cgtacgccgc ctggaacatg gcgacgatga
     1981 tgtcgttctg atcgctcgtc ggcgtgccga tagctctgcc ctggcgatta tgaatgccat
     2041 cctgca
//
LOCUS       DAOTIL010000514         2044 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminobacteriaceae bacterium isolate AMR_MDS_3026
            NODE_65117_length_2044_cov_3.093514, whole genome shotgun sequence.
ACCESSION   DAOTIL010000514 DAOTIL010000000
VERSION     DAOTIL010000514.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868050
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminobacteriaceae bacterium (wastewater metagenome)
  ORGANISM  Aminobacteriaceae bacterium
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae.
REFERENCE   1  (bases 1 to 2044)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2044)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:19:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,224
            CDSs (total)                      :: 3,198
            Genes (coding)                    :: 3,184
            CDSs (with protein)               :: 3,184
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2044
                     /organism="Aminobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_65117_length_2044_cov_3.093514"
                     /isolate="AMR_MDS_3026"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:3034609"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..622
                     /locus_tag="P5201_10210"
     CDS             <1..622
                     /locus_tag="P5201_10210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013048290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="magnesium chelatase domain-containing protein"
                     /protein_id="HRV98956.1"
                     /translation="PKHLKEYLGPPRLYDPRLPKTKEIGTVVGLAWTETGGDVLVIEA
                     VTMKGKGEVTLTGNLGSIMQESAQTAIGFLRSNADGFGIGNIDWKAIDVHLHVPEGAI
                     PKDGPSAGITMAVAILSAVRKSSVLPNIAMTGEVSLQGKVLPVGGIRDKILAAKRQGI
                     KNIILPLANKPDVEEIPEWSRDGLSFRYVDRVENVFEYALERAPSP"
     gene            619..1218
                     /gene="yihA"
                     /locus_tag="P5201_10215"
     CDS             619..1218
                     /gene="yihA"
                     /locus_tag="P5201_10215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013048289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTP-binding protein
                     YihA/YsxC"
                     /protein_id="HRV98957.1"
                     /translation="MNSKWKVSLECTAFTADQFPPPEIPEIAVAGRSNVGKSTLINNL
                     LGARLAHVGATPGKTRSVNFFLVEREGVPFRLVDLPGFGYASRSKAERNTWSRLIESY
                     VASRKSLWLVLHLVDFRHGLLENDASLQEWLDGIGLPVFVVFTKGDKIAKGKHLSTRD
                     QFIRKGMKSIDVPIITSGDKGTGIQELRVFIESFLDRQE"
     gene            1299..>2044
                     /locus_tag="P5201_10220"
     CDS             1299..>2044
                     /locus_tag="P5201_10220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013048288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I tRNA ligase family protein"
                     /protein_id="HRV98958.1"
                     /translation="MADIMESMAKSYNPEPIEDKWYARWIDSDLFKARVDKSKEAFSI
                     VIPPPNVTGSLHMGHAFNHTFQDIVCRFKRMRGYNVLWLPGTDHAGIATQNVVERRLA
                     GEGKSRHDLGRDEFVQRVWEWKDEFGSRIINQMKKLGNSCDWDRERFTLDEGLSKAVR
                     SVFVQLYKKGLIYKGKYIINWCSRCHTALSDIEVEHEECKGRLYYVRYPFVDGEGSVV
                     VATTRPETIWGDVAIAVHPRSEKGALVGRKV"
BASE COUNT          474 a          515 c          602 g          453 t
ORIGIN      
        1 ccccaaacac ctgaaggagt acctcggccc accgagactg tacgatcccc gtcttccgaa
       61 gacgaaggag atcggcaccg tcgtggggtt ggcatggacg gaaaccggcg gcgatgtgct
      121 cgtcattgag gctgtcacga tgaagggaaa aggagaagtc acccttacag ggaatcttgg
      181 ctccatcatg caggaatccg cgcagaccgc gataggtttt ctccgttcga atgcggatgg
      241 ttttggcatc ggaaatatcg actggaaggc tatcgacgtc catcttcatg tcccggaagg
      301 cgctattccc aaagacggcc cctccgcagg aatcaccatg gccgtagcca ttctttccgc
      361 ggttcgcaag agctcggtgc ttccaaatat cgccatgaca ggcgaggtat ctcttcaggg
      421 aaaggtgctc cccgtcgggg gtatccggga caagatattg gctgctaaac gacaggggat
      481 caagaacatc attcttcctc tggcaaacaa gcctgatgtt gaagaaatac ctgagtggtc
      541 gcgggatggt ctttctttcc gttatgttga ccgggtggag aacgtgtttg agtatgccct
      601 tgaaagggcg ccctctccat gaactcgaag tggaaagttt cgctcgagtg caccgcgttt
      661 acggcagacc agtttcctcc tccggagatt ccggagatcg cggtggccgg acgatcgaac
      721 gtagggaagt cgacgcttat caacaacctg ctcggggcac ggttggccca tgtgggggcg
      781 actcccggga agacccggag cgtcaatttc tttcttgttg agcgggaagg agtcccgttc
      841 aggctggtag atcttccggg atttggatat gcctcccgga gcaaagcgga gcggaacacg
      901 tggtcccgcc ttatcgaatc gtatgttgcc agccggaaga gtctttggct tgtgctgcat
      961 cttgtcgatt tccggcacgg tctcctggaa aatgatgcat cgcttcagga gtggctcgat
     1021 ggcataggcc ttcccgtttt cgttgtcttt acaaaagggg acaaaatagc gaaaggaaag
     1081 cacctgagta cgcgagacca gttcatccgt aaggggatga aatcgatcga cgtccccatc
     1141 atcacctcag gcgataaggg gactggcatt caggagctta gggtatttat cgagtcattt
     1201 cttgaccgtc aggagtaaca gatgtttccc ccgcaggggg attctattga aatactgccg
     1261 aggaagcaaa tttttaggca acggagggga attgcagcat ggcagacatt atggaatcta
     1321 tggcgaaatc gtacaacccc gaaccgatcg aagataaatg gtatgcgcgc tggattgatt
     1381 cggatctttt caaggcgcgc gtcgacaaga gcaaagaggc gttttctatc gtaattcctc
     1441 cgccgaatgt cacaggatcg cttcacatgg ggcatgcctt caatcatacg ttccaggata
     1501 ttgtctgccg cttcaaacgt atgcgcgggt ataacgtcct ctggctgccc ggcaccgacc
     1561 atgcagggat tgccacgcag aacgtcgtgg aacgccgtct ggcgggcgaa gggaagtctc
     1621 gccacgatct cggcagggat gaattcgtac agcgggtctg ggagtggaag gacgagttcg
     1681 gtagtcggat catcaaccag atgaagaagc tgggaaactc ctgcgactgg gacagagagc
     1741 gttttacgct cgacgagggg ctctcgaagg ccgtccgttc cgtcttcgtt cagctctaca
     1801 aaaaaggact catttacaag ggaaagtaca tcatcaactg gtgctcacgt tgccatacgg
     1861 cgctctccga tatcgaggtt gagcacgaag agtgtaaagg gcgcctttac tatgtacgct
     1921 atcctttcgt cgacggcgag gggagcgttg tcgtcgcaac gacacgtcct gagacgatat
     1981 ggggcgacgt ggcgatcgca gtccatcccc gctcggaaaa aggcgccctc gttggaagaa
     2041 aggt
//
LOCUS       DAOTIL010000515         2035 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminobacteriaceae bacterium isolate AMR_MDS_3026
            NODE_65505_length_2035_cov_3.321212, whole genome shotgun sequence.
ACCESSION   DAOTIL010000515 DAOTIL010000000
VERSION     DAOTIL010000515.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868050
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminobacteriaceae bacterium (wastewater metagenome)
  ORGANISM  Aminobacteriaceae bacterium
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae.
REFERENCE   1  (bases 1 to 2035)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2035)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:19:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,224
            CDSs (total)                      :: 3,198
            Genes (coding)                    :: 3,184
            CDSs (with protein)               :: 3,184
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2035
                     /organism="Aminobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_65505_length_2035_cov_3.321212"
                     /isolate="AMR_MDS_3026"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:3034609"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..333
                     /locus_tag="P5201_10225"
     CDS             <1..333
                     /locus_tag="P5201_10225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013048291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit
                     ClpX"
                     /protein_id="HRV98959.1"
                     /translation="VPLEELNTDALIRILVEPKNSIVRQYQKTFSMEGVSLEFTEDAL
                     RSIALKAGEKNTGARGLRAILENLMMDLMYDIPSRGKDVGKVIITKEAVEGSQPPLEE
                     ARSSEEVA"
     gene            338..>2035
                     /gene="lon"
                     /locus_tag="P5201_10230"
     CDS             338..>2035
                     /gene="lon"
                     /locus_tag="P5201_10230"
                     /EC_number="3.4.21.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014163217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endopeptidase La"
                     /protein_id="HRV98960.1"
                     /translation="MKKEEEAQAQDIQDITEVSASGPRSCFVLPVRDIVIFPGIIAPL
                     FVGRPRSLKAIEMAMLQDRNVLVVAQKEMQVDDPRVEDLYSVGTVCSILQMVRIPDGT
                     TKVLIEGVERVQVVSYGKGKETLEADVVSLPWEEHHSPNLEALKRSVLEQFEKYVTLH
                     PRIPGEVLISVMNVEDPWQISDLVGSHLSLKVEKKQKLLEMVNLEKSLKFLLKVLLEE
                     IDILQLEHAIQDKVRQEVERGHKEYYLREQLKVIQDELGQGEKPSEIDELRTRIEEAE
                     MPEASLSKALHELDRLSKMPLMSAEATVVRTYIDWLVTLPWNTMTPDNLEIKRAQKVL
                     DEDHYGLEKVKERILEFLAVRKLALDKMKGQVLCFVGPPGVGKTSLGKSIAKALERKF
                     VNMSLGGMRDEAEIRGHRRTYVGAMPGRILQKIRQAGARNPVLLMDEIDKLGLDFRGD
                     PAAALLEVLDPEQNSSFTDHFMEVPFDLSDVIFITTANVTHSIPRPLLDRMEVIKIPG
                     YVWQEKIKIAKNHIFPRILEEHGLTPKQLKITPSGLEHVISEYTREAGVRGLERELST
                     "
BASE COUNT          523 a          505 c          599 g          408 t
ORIGIN      
        1 gttccccttg aagagctgaa tacggatgcg ctgatccgta ttcttgttga gccgaagaac
       61 tctattgttc gtcagtatca gaagaccttc tccatggaag gggtgtcctt ggagttcacc
      121 gaggatgctc ttcgaagcat cgccttaaaa gccggggaga aaaacacagg cgccagaggg
      181 cttcgcgcaa tcctcgagaa cctgatgatg gacctgatgt acgatatccc ctccagggga
      241 aaggacgtcg gcaaggtgat cataaccaaa gaggccgtcg aaggatcgca gccccctctg
      301 gaagaagcga gaagctcgga ggaggttgcc tgatatcatg aagaaagaag aagaagcgca
      361 ggcgcaggat atacaggata taacggaggt ttccgcttcc ggtccgcgca gctgttttgt
      421 ccttccggta cgggatatcg ttatcttccc cggtatcatc gcgccgcttt tcgtggggcg
      481 tccccgttcc ctgaaggcaa tcgagatggc catgctccag gacaggaatg tcctggtagt
      541 cgctcaaaaa gagatgcagg ttgacgaccc tagggtagag gatctctatt cagtgggcac
      601 tgtatgcagc attctccaga tggtcaggat tcctgacgga acgacgaaag tgctcatcga
      661 gggcgtggag cgcgttcagg ttgtttcgta cggtaaggga aaagagactc tggaggctga
      721 tgttgtatcc ctgccgtggg aagagcacca ctccccgaac ctggaagctt tgaagaggag
      781 cgttctggaa cagtttgaga aatacgtgac gctccacccg cggattccgg gcgaagtgct
      841 tatttcagtg atgaacgtag aggatccctg gcagatttcc gatttggtcg gctcgcacct
      901 ctcgctgaag gtggaaaaga aacagaaact gctcgagatg gtcaatcttg aaaagagtct
      961 gaagtttctc ctgaaggtcc tcctggaaga gatcgatatc ctacaactgg agcacgctat
     1021 ccaggacaag gtgcgccagg aagttgagcg ggggcataag gagtactacc tcagggagca
     1081 gctgaaagta atccaggacg aactcggaca gggtgaaaaa ccttccgaga tcgatgagtt
     1141 gcggacgcgc atagaggaag cggagatgcc ggaagcctct ttgagcaaag cgcttcacga
     1201 gttggaccgg ctttcaaaga tgccgctcat gtccgcagag gcaaccgttg tacggacgta
     1261 catcgactgg cttgtgaccc tcccgtggaa tacgatgacg ccggacaatc tggagataaa
     1321 aagagcgcag aaagtccttg acgaagacca ctacggcctg gagaaagtga aggagcgtat
     1381 cctggaattc ctggccgtcc gcaagctcgc gctggacaag atgaaggggc aggtactctg
     1441 ctttgtcggc cctccgggag tgggaaagac ctcgctcgga aaatcgatcg caaaggcgct
     1501 cgaacgcaaa tttgtcaata tgtcgcttgg cggtatgcgg gacgaagccg agattcgcgg
     1561 ccacagaaga acctatgtcg gcgcaatgcc gggccgaatt ttacagaaga tacgacaggc
     1621 aggcgcacgg aacccggtgc ttttgatgga cgagatcgac aaactcggac ttgacttcag
     1681 gggagatcct gcagccgcgc ttctggaggt acttgatccg gaacagaaca gctcctttac
     1741 cgaccacttc atggaggtgc cgttcgatct gagcgacgtc atcttcatca cgacagccaa
     1801 tgttacccac tctatcccca gacctctgct cgacagaatg gaggtcatca aaatcccggg
     1861 gtacgtatgg caggagaaga taaagatagc gaaaaaccat atcttcccga gaattcttga
     1921 agagcacggg cttactccga agcagttgaa gataaccccc agcgggctgg aacatgtcat
     1981 cagcgagtac acccgggaag cgggagtccg cggcctggaa cgagagctct ccacc
//
LOCUS       DAOTIL010000516         2030 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminobacteriaceae bacterium isolate AMR_MDS_3026
            NODE_65734_length_2030_cov_2.623291, whole genome shotgun sequence.
ACCESSION   DAOTIL010000516 DAOTIL010000000
VERSION     DAOTIL010000516.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868050
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminobacteriaceae bacterium (wastewater metagenome)
  ORGANISM  Aminobacteriaceae bacterium
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae.
REFERENCE   1  (bases 1 to 2030)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2030)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:19:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,224
            CDSs (total)                      :: 3,198
            Genes (coding)                    :: 3,184
            CDSs (with protein)               :: 3,184
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2030
                     /organism="Aminobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_65734_length_2030_cov_2.623291"
                     /isolate="AMR_MDS_3026"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:3034609"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1041
                     /locus_tag="P5201_10235"
     CDS             <1..1041
                     /locus_tag="P5201_10235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013048163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate synthase"
                     /protein_id="HRV98961.1"
                     /translation="FQADEAPEQEAAQPLRTPPVSKAFSPGSSLSATPPRAEQLPGVT
                     ATKLLLNIEGPAAIDRLASLPADGVGLLRTEFIFSGEIGTHPMHLVRTKQGSLFVDKL
                     AEGMSTVARAFFPRPVLARFSDFRTNDFRKLNGGQELEPVESNPMIGWRGASRYISPD
                     YEEGFRLECRAVKKVRDEMGNSNLSVIIPFVRTLKEMERVKAILASEGLVSGASFKIW
                     LMAEVPSVVFQADAFASLVDGITIGTNDLTQLILGVDRDSAALENMGYFDERNPAVLH
                     AVSLVIEAARRAGIPCSVCGEGPSVFPDFAEHLVREGIDSISVNADALEYTRRIVASV
                     EQKIILDALRGK"
     gene            complement(1133..>2030)
                     /locus_tag="P5201_10240"
     CDS             complement(1133..>2030)
                     /locus_tag="P5201_10240"
                     /inference="COORDINATES: protein motif:HMM:NF013100.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="penicillin-binding transpeptidase
                     domain-containing protein"
                     /protein_id="HRV98962.1"
                     /translation="MGVVSQHLPADLSLALGTASVTPLEMAAVFSVFANNGKAVDPLM
                     IREIRSSNGDILETRRPYAREALSVEAAVTVRSMLLDAVRAGTGRKALIPDRETFGKT
                     GTTNDFTDAWFLGGVPGLVAVVYAGNDDHTPLGKRNVTGGDVSAPVWNAFVSAAVKTV
                     ELPKKFDIPSDADVEAVSICRTTGFRALKGCSAATVYMRKGAAPQTSCPIHGGDMNAS
                     TLDPNAPRLVLVHNDSELHTGDLYAAGMQPVETPPVAVTAQKQKQEVVVPESTGAYKD
                     PNPAVTVEDRYQQLLKQYGLSN"
BASE COUNT          409 a          669 c          564 g          388 t
ORIGIN      
        1 ttccaggcgg acgaagcacc cgaacaggaa gcggcgcagc cccttcggac gccccctgtt
       61 tcaaaggctt tttcaccggg aagctctctt tccgcaacgc ctcccagggc agaacaactc
      121 ccgggagtca ccgcgacaaa gctgcttctc aacatagagg gtcctgcggc aatcgaccgc
      181 ctggcttcac tgcctgcgga cggcgtcggc cttctccgta cggaattcat cttttcaggc
      241 gagatcggaa cgcacccgat gcacctcgta cgcacaaagc aggggagcct ctttgttgac
      301 aaactcgcgg aaggcatgag cacggttgcg cgcgcgttct tcccgcgtcc cgtccttgcc
      361 cgcttcagcg acttccgcac caacgacttc cggaagctca acggagggca ggaactcgaa
      421 ccggtcgagt ccaacccgat gataggatgg agaggagcct cccgctacat ctcccccgac
      481 tacgaagagg gcttccggct cgaatgcagg gccgtcaaaa aagtccggga tgaaatgggc
      541 aactccaacc tctcggtcat catccccttt gtccgcaccc tcaaggagat ggagcgggtc
      601 aaggccatcc tcgcctccga agggctcgtt tccggcgctt cgttcaagat ctggctcatg
      661 gcggaagtac cctctgttgt cttccaagca gatgccttcg cctcactggt cgacggcatc
      721 accatcggaa caaacgacct cacgcagctt atcctcggcg tcgacagaga ctcggcagcc
      781 ctggagaaca tggggtactt cgatgaacgc aatcctgcgg tgcttcacgc cgtttccctc
      841 gtgatcgaag ccgctcgccg cgccggaata ccgtgttccg tctgcggcga aggcccctca
      901 gtctttcccg acttcgccga acacctggtc cgcgaaggca tcgacagcat cagcgtcaat
      961 gcggacgcac tggagtacac tcgccgcatc gtcgcatcgg tcgaacaaaa gatcatcctc
     1021 gacgcccttc gcggcaagta acgtcaggga cgcgcaacgc aaagaaaagg agcgccggga
     1081 ccctgtgaat gcaggagttc cggcgctctt tgctcattga gggaaaagat gctcagttgg
     1141 acaggccgta ctgcttcaaa agctgctggt atctgtcctc cacagtcacg gcagggttcg
     1201 gatccttgta cgcgcccgtt gactccggaa cgacgacctc ctgcttctgt ttctgcgcgg
     1261 tcaccgcgac cggaggggtt tcaaccggct gcattcctgc tgcatacaga tccccggtat
     1321 gcagctcgga gtcgttatgc accagcacca ggcgaggagc gttcgggtcc agcgtactcg
     1381 cgttcatgtc ccctccatga atcgggcatg acgtctgcgg cgcggcgccc ttccgcatgt
     1441 acaccgttgc tgccgaacag cccttaagag cgcggaagcc tgtcgtgcgg cagatggaaa
     1501 ccgcctcgac atcagcgtcc gaagggatat cgaatttttt cggaagctcg accgtcttca
     1561 ctgcggccga gacaaaggcg ttccacaccg gagcggagac gtctcccccg gtaacgttgc
     1621 gcttccccaa gggcgtatgg tcgtcgttcc ccgcgtacac cacggccacc aggccgggaa
     1681 ccccgccgag gaaccacgcg tcggtaaagt cgttcgttgt tcccgtcttg ccgaacgtct
     1741 cgcggtcggg aatgagcgct ttccgtcccg tccccgcgcg aacggcatcc agcagcattg
     1801 aacgaaccgt caccgccgcc tctactgaaa gagcctccct cgcgtacggc ctgcgcgttt
     1861 ccagaatgtc gccgtttgaa gagcggattt ccctgatcat cagagggtct acggctttcc
     1921 cgttgtttgc gaacaccgaa aaaacagccg ccatctcaag cggcgttacg ctcgcggttc
     1981 caagggccag agaaaggtcg gccggaagat gctgcgatac aacccccatc
//
LOCUS       DAOTIL010000517         2024 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminobacteriaceae bacterium isolate AMR_MDS_3026
            NODE_65992_length_2024_cov_2.923819, whole genome shotgun sequence.
ACCESSION   DAOTIL010000517 DAOTIL010000000
VERSION     DAOTIL010000517.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868050
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminobacteriaceae bacterium (wastewater metagenome)
  ORGANISM  Aminobacteriaceae bacterium
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae.
REFERENCE   1  (bases 1 to 2024)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2024)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:19:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,224
            CDSs (total)                      :: 3,198
            Genes (coding)                    :: 3,184
            CDSs (with protein)               :: 3,184
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2024
                     /organism="Aminobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_65992_length_2024_cov_2.923819"
                     /isolate="AMR_MDS_3026"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:3034609"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..476)
                     /locus_tag="P5201_10245"
     CDS             complement(<1..476)
                     /locus_tag="P5201_10245"
                     /EC_number="2.7.8.26"
                     /inference="COORDINATES: protein motif:HMM:NF014685.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008818 - cobalamin 5'-phosphate synthase
                     activity [Evidence IEA];
                     GO_function: GO:0051073 - adenosylcobinamide-GDP
                     ribazoletransferase activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylcobinamide-GDP ribazoletransferase"
                     /protein_id="HRV98963.1"
                     /translation="MGILEGFFRALSFLTILPCPKRESTVEEYGRASGWFPVAGAVLG
                     AILYAAALLFHRSFSPLPASALVVALWAFLTRGFHLDGLADTFDGIGGGFDRESRLAV
                     MKDSRLGTFGGTALAAALLLKTVILAEFGGRLEVLVIAPALGRWGILIAMRFFPSAR"
     gene            complement(478..1560)
                     /gene="cobT"
                     /locus_tag="P5201_10250"
     CDS             complement(478..1560)
                     /gene="cobT"
                     /locus_tag="P5201_10250"
                     /EC_number="2.4.2.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014162320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008939 -
                     nicotinate-nucleotide-dimethylbenzimidazole
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate-nucleotide--dimethylbenzimidazole
                     phosphoribosyltransferase"
                     /protein_id="HRV98964.1"
                     /translation="MTTLQQTIDKIRPLCAESMEAARRRQDVLTKPKGSLGRLEELSV
                     RLAGIQRTVHPVIRDKVIMTMAGDHGVVSEGVALYPKEVTVQQVLNFLRGGGGVNVLG
                     RHAGARVVLVDMGVDYDFEPMEGLQIRKVARGTASMLRGPAMTREQAVQALENGIAAV
                     YEEAVKGMDILGTGEMGIGNTTPSTAIACAVTGVAVPLATGRGAGLTDDGLKRKIEVI
                     EKSLALHSPDPKDGIDVLSKVGGFEIGGIAGAILGAASLGIPVVVDGFISTAGAVIAA
                     LLCPRAKEYMIYSHNSVEIGHEHMFSFLEARPLLDLGMRLGEGTGAALAISIVEGAVK
                     VLNEMATFESAGVSGPVDSEGVADEG"
     gene            complement(1646..>2024)
                     /locus_tag="P5201_10255"
     CDS             complement(1646..>2024)
                     /locus_tag="P5201_10255"
                     /inference="COORDINATES: protein motif:HMM:NF014351.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="bifunctional adenosylcobinamide
                     kinase/adenosylcobinamide-phosphate guanylyltransferase"
                     /protein_id="HRV98965.1"
                     /translation="RRPSAWRTVEEPYGIEGVVASLEAGSVALVDCLSLWVSNLLLMT
                     PEQGDSGIADSVLGRVRAFLDAAKRSSGDVIVVSNETGCGVVPSSHLGRLYRDILGWA
                     NQEAAQAADEVWFLVAGLPQRLK"
BASE COUNT          375 a          715 c          594 g          340 t
ORIGIN      
        1 cgggcggacg gaaagaaccg catcgcgatg aggatgcccc accgcccaag cgccggggcg
       61 atgacaagca cctcaagccg tccgccgaac tccgcgagaa tgaccgtctt caacagcagc
      121 gctgcggcga gggccgtccc cccgaaggtc cccaggcggc tgtccttcat gacggcgagc
      181 cgcgattccc ggtcgaaacc gccgccgatg ccgtcgaacg tgtccgccag tccgtcgagg
      241 tggaaccctc tcgtcaaaaa cgcccagagg gccacaacaa gcgccgacgc gggcaggggc
      301 gaaaaggaac gatggaagag cagcgcggcg gcatacagaa tcgcccccag cacggccccc
      361 gccacaggaa accatccgct cgcccgcccg tactcctcca ccgtgctctc gcgcttcgga
      421 cacggaagaa tcgtcaggaa gctcagcgca cggaaaaaac cctcaaggat tcccatatca
      481 gccctcgtcg gcaactcctt ctgagtcgac cggtcccgag acgcccgcgc tctcgaatgt
      541 ggccatctcg ttgagcacct tcacggcgcc ttcgacgatc gaaattgcga gcgccgctcc
      601 ggttccctcg cccagccgca tgccgaggtc gagcagcggc ctcgcttcca ggaagctgaa
      661 catgtgctcg tgccctatct ccaccgagtt gtgcgagtag atcatgtact ccttcgccct
      721 cgggcagaga agcgccgcga tcaccgcgcc cgccgtggag atgaagccgt ccacgaccac
      781 agggataccc agcgacgccg cgccgagaat cgccccggcg ataccgccga tctcgaaccc
      841 tccgaccttg gagagcacgt cgataccgtc cttcgggtcg ggactgtgca gggcgagcga
      901 cttttcgatg acctcgatct tgcgtttcag cccgtcgtcg gtgagccccg ctcccctgcc
      961 cgtggcgagg ggaacggcga cgcccgtcac ggcgcaggcg atcgccgtcg atggcgtggt
     1021 gttgccgatg cccatctccc cggttccgag gatgtccatc cctttgaccg cctcttcgta
     1081 cacggcggcg attccgttct caagagcctg aacagcctgc tccctcgtca tcgccggccc
     1141 cctgagcatg ctcgccgttc cgcgcgccac cttgcgtatc tgcaagcctt ccataggctc
     1201 gaagtcgtaa tcgaccccca tgtcgacaag aacgacgcgt gctccggcat gacggccaag
     1261 cacgttcacg cccccgccgc ctcgcaggaa gttgagcacc tgctggacgg tcacctcttt
     1321 ggggtagagc gccacgccct cggaaacgac gccgtgatcc cccgccatag tcataatgac
     1381 cttgtcgcgg atcaccgggt ggacagtccg ctgaataccg gcgagcctga ccgaaagctc
     1441 ttcgagcctg ccgaggctcc ccttcggctt ggtgagcacg tcctggcggc ggcgcgccgc
     1501 ctccattgat tcagcgcaca gcgggcggat tttatcgatt gtctgttgaa gagttgtcat
     1561 gatgcctcct aagaatttcc cgcgtgcacg ggagatgatt gcgcaaggac gtgcgtgagt
     1621 acgcacgttt ccgttgcgtg ctacgttatt tcagccgctg cggcagtccc gcgacgagga
     1681 accagacctc gtcggcggct tgagccgcct cctggttggc ccagcccaga atgtcgcggt
     1741 agagtcttcc aaggtgagaa gaaggaacga cgccgcaccc cgtctcgttg gagacgacga
     1801 tcacgtcgcc ggaggagcgt ttcgccgcgt cgagaaacgc ccggactcgt cccagaacgg
     1861 aatctgcgat tccggagtcg ccctgctccg gcgtcatgag aaggaggttg ctcacccaga
     1921 ggctcaggca gtccacgagc gcgacactcc cggcctccag cgatgcaacg acgccctcta
     1981 ttccgtacgg ctcttccacg gtcctccacg ccgacggacg ccgc
//
LOCUS       DAOTIL010000518         2022 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminobacteriaceae bacterium isolate AMR_MDS_3026
            NODE_66069_length_2022_cov_4.041179, whole genome shotgun sequence.
ACCESSION   DAOTIL010000518 DAOTIL010000000
VERSION     DAOTIL010000518.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868050
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminobacteriaceae bacterium (wastewater metagenome)
  ORGANISM  Aminobacteriaceae bacterium
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae.
REFERENCE   1  (bases 1 to 2022)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2022)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:19:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,224
            CDSs (total)                      :: 3,198
            Genes (coding)                    :: 3,184
            CDSs (with protein)               :: 3,184
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2022
                     /organism="Aminobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_66069_length_2022_cov_4.041179"
                     /isolate="AMR_MDS_3026"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:3034609"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..229
                     /locus_tag="P5201_10260"
     CDS             <1..229
                     /locus_tag="P5201_10260"
                     /inference="COORDINATES: protein motif:HMM:NF013696.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HRV98966.1"
                     /translation="AAEKACGEMGVSCVRMPSGASHDAAPMAGIVPAGMLFVPSAGGI
                     SHAKEEATPPDDLVRGAEALLRTVLEADARL"
     gene            410..1456
                     /locus_tag="P5201_10265"
     CDS             410..1456
                     /locus_tag="P5201_10265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005661878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1905887 - autoinducer AI-2 transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HRV98967.1"
                     /translation="MEKFQVMIGLVGVIALVAVGFVLNFAQAVFIPLIIAWLLSYLFG
                     PIVRYLAKRRVPVGLAVVFVLAIFFGLCFLGAIFLNTRIASFAAAFPKYYSRLMEIGK
                     SLTTNLDIPPDFWTSINWGFTIRGYLLDLSSSFLTIISKLVMVIVFLVFMLLGSPYFE
                     YKVKKAFVTPHGSERARKILSTISWQIGRYLSALALISAVTGLIVWMSLEYLQVDFAV
                     TWGVLAFILNFIPTIGSIVASIPPILVAVVQFYPSLTAPLITALTLLTIQVVIGNFIT
                     PKVMGDRLNLSPVVVLISLLFWGLIWGVVGALISVIIAAIIKIVCENFPMLNVISVMM
                     GSGRIYQKEFEEKR"
     gene            complement(1509..>2022)
                     /locus_tag="P5201_10270"
     CDS             complement(1509..>2022)
                     /locus_tag="P5201_10270"
                     /inference="COORDINATES: protein motif:HMM:NF013887.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="3-dehydroquinate synthase"
                     /protein_id="HRV98968.1"
                     /translation="KPFSPRELVARVRAILRRSLPALRTLVAECSRMKLSVVAEDEHE
                     RSGARARLNLGHTVGHALEAASGYRWRHGEGVAAGMMVAGRLAHRLGEFRKEEPARLE
                     RLLEAFRIPCRPDMPWDEIAPFLARDKKFTGGAPRLVLPRTGERCRLRDDVALAALRN
                     AYEEELLKAR"
BASE COUNT          346 a          628 c          582 g          466 t
ORIGIN      
        1 ggcggccgaa aaggcatgcg gcgagatggg cgtctcctgc gtcagaatgc cgagcggcgc
       61 gtcgcacgac gcagccccga tggccggcat cgtcccggcc gggatgctct tcgttcccag
      121 cgcgggcggt atcagccatg ccaaagaaga agcgaccccg ccggacgatc ttgttcgagg
      181 ggcggaggcc cttctgagga ctgtgcttga agctgacgcg cgcctgtaga caggaagcgg
      241 cggactccgc cttttccccg aatgcggcgg agtctgcaga tgtgtgtttt ccttcttttt
      301 tcagcgcgat gtatttttta ttcacccttt tctcccaaaa cggattcctc agtgtagaat
      361 aataagtgtg gaggggtctg ctcgcatctc aaggaaggga agggatagtg tggaaaaatt
      421 tcaggttatg atcggcctcg tgggagtgat cgctctcgtc gccgttggtt ttgtgctgaa
      481 cttcgcacag gcggtcttca ttccgcttat tattgcatgg cttctttcgt atctttttgg
      541 tcccatcgtt cgttacctcg ccaaacgccg cgttcctgtg gggcttgcgg tggtttttgt
      601 ccttgccatt tttttcggtc tctgcttcct cggcgctatc tttctgaata cccgaatcgc
      661 ctcgttcgcg gccgcctttc cgaagtacta ctcgcgcctg atggagatag gcaagtcgct
      721 tacgacgaat ctcgatattc ctccggactt ttggacgtcc atcaactggg ggttcaccat
      781 cagagggtat ctcctcgatc tctccagctc cttcctcacc atcatctcga aacttgttat
      841 ggtcatcgtc ttcctcgtct ttatgctgct cggctctccg tatttcgaat acaaggtgaa
      901 gaaagccttt gtcacgcccc acggctccga acgtgcacgc aagattctga gcaccatatc
      961 gtggcagata gggcgttacc tcagcgcttt ggcgctcatc agcgcggtga ccgggctgat
     1021 cgtctggatg tcccttgaat acctgcaggt ggatttcgct gtaacgtggg gagtgctcgc
     1081 tttcatcctc aacttcatcc ccacgatcgg ctcgatcgtc gcgtccattc ctccgatcct
     1141 cgtggccgta gtccagttct acccgagcct gacagccccc ctgatcaccg cgctcacgct
     1201 gctcaccatt caggtggtca tcggcaactt catcacgccg aaggtcatgg gcgaccggct
     1261 caacctcagc ccggtagtgg tcctcatctc gctgctcttc tggggactca tctggggcgt
     1321 ggtcggcgcg ctcatctcgg tgatcatcgc cgcgatcatc aagatcgtct gcgagaactt
     1381 cccgatgctc aacgtgatca gcgtgatgat gggttccgga agaatctacc agaaggagtt
     1441 cgaggagaag cgctaacacg tccggacacc tgcggcggaa tatgaagaga ggagctcccc
     1501 ggcgcgtttc agcgcgcctt gaggagctcc tcttcgtatg cgttccgcag cgcggcgagc
     1561 gcgacatcgt cccgcaggcg gcagcgttcg cccgtgcggg ggagtaccag gcggggagcg
     1621 ccaccggtaa atttcttatc ccgtgccaga aacggggcga tctcgtccca cggcatatcc
     1681 ggacggcatg gaatcctgaa cgcctccaga agccgttcca gacgagccgg ctcctccttc
     1741 cggaattcgc cgaggcggtg cgccagcctt ccggcaacca tcatccccgc ggcgacgcct
     1801 tcgccgtgcc gccatctgta gccggacgcg gcttcaagcg cgtgtccgac ggtgtgcccg
     1861 aggttcagac gggcccgcgc cccgctccgt tcatgctcgt cctccgcgac cacggagagc
     1921 ttcatccgcg aacactccgc gacgagcgtc ctcagtgcgg gcagagagcg gcgcaggatg
     1981 gcgcgcaccc gggccaccag ttcgcgcggg ctgaagggct tg
//
LOCUS       DAOTIL010000519         2016 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminobacteriaceae bacterium isolate AMR_MDS_3026
            NODE_66335_length_2016_cov_3.575217, whole genome shotgun sequence.
ACCESSION   DAOTIL010000519 DAOTIL010000000
VERSION     DAOTIL010000519.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868050
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminobacteriaceae bacterium (wastewater metagenome)
  ORGANISM  Aminobacteriaceae bacterium
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae.
REFERENCE   1  (bases 1 to 2016)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2016)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:19:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,224
            CDSs (total)                      :: 3,198
            Genes (coding)                    :: 3,184
            CDSs (with protein)               :: 3,184
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2016
                     /organism="Aminobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_66335_length_2016_cov_3.575217"
                     /isolate="AMR_MDS_3026"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:3034609"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1467
                     /locus_tag="P5201_10275"
     CDS             <1..1467
                     /locus_tag="P5201_10275"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HRV98969.1"
                     /translation="RHFDGIANVKLLREITRHLFAETTDLLFLFTLEGKLREISRSAS
                     DELGYPEEDALSLNFFADLLAVPSPVKMEQLCKSLLDGQDVTLAGTVRRRDGSGCAVQ
                     LTVFRPGEGREALFLCSAKKPEEEKPPRDTGQKVALRDPVTGLSTRAFFFEELQQMDS
                     ERYLPLSVIRGDLNGMKLINSVFGHIAGDKLLKAAARVLRKHSRNSDILVRWEGDEFL
                     LLLPRTKEDDAATIVERIEDAFKKTKIREIPVPPSISMGYSAKVHRWQDFGNVLKDAE
                     EDMREKQLSESRKIRTKLLSSLQKSLDEHTPETEEHISSMRRIARIMSDLLQLSPEER
                     ELLDLAVRFHDIGKASIPVYLLNKIAPITDEDRETLRRHAEIGGKIAEAATSETAKSA
                     PVIRAHHERWDGTGYPSGIAGEDIPLLSRIISIADAFDVMTRGTAYKDAVSPEEALEE
                     LERLAGLQFDPRLVDLFAEHAEKFLTDESPETLPERRK"
     gene            1528..1959
                     /locus_tag="P5201_10280"
     CDS             1528..1959
                     /locus_tag="P5201_10280"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="thioesterase"
                     /protein_id="HRV98970.1"
                     /translation="MLDLSAILQPGLAGEVKKTVDLDDTTGTSSPYLGQYLSTSACAA
                     LAVHASMAATEGRLPEGYLSVGRRLDLEHDAPAMTGTTVIVKATLREIRGNRLIFDIL
                     GSDALGVIFKGVNERVVVNRIGLEERGTERAQQLKALREKL"
BASE COUNT          463 a          593 c          588 g          372 t
ORIGIN      
        1 cggcatttcg acgggatcgc caacgtgaaa ctcctgcgtg agattacacg gcatcttttc
       61 gcggagacaa ccgacctgtt attcctcttc accctcgaag ggaagctgcg cgagatcagc
      121 cgatcggcaa gcgacgagtt gggatacccc gaagaggacg cgctctcgct gaattttttc
      181 gccgatcttc tcgccgttcc ctctccggta aaaatggaac aactctgtaa atccctcctc
      241 gacgggcagg atgttaccct cgcaggaaca gtccggcgcc gcgacggaag cggctgcgca
      301 gtgcagctca cggtcttccg cccgggcgag ggaagagaag cgctctttct ttgttcggcg
      361 aaaaagcccg aagaagaaaa gcccccccgc gacaccggac agaaagtcgc gctacgcgat
      421 ccggtaacgg ggctctccac tcgcgccttt ttcttcgagg agctgcaaca gatggactct
      481 gaacgctacc tgccgctgag cgtcatccgc ggagacctga acggtatgaa gctgatcaac
      541 agcgtctttg ggcacattgc gggggacaag ctcctcaagg ctgcggccag ggttctccgg
      601 aaacattcga gaaacagcga tatcctcgtc cgctgggagg gcgacgaatt tttgcttctg
      661 ctcccgagga cgaaggaaga tgacgctgca acaatcgtcg agcgcataga ggacgcgttc
      721 aaaaagacaa aaatcagaga gatcccggtt cctccgagta tttccatggg gtactccgcc
      781 aaagtgcacc gatggcagga cttcggcaac gtcctcaaag acgcggagga agacatgcgc
      841 gagaagcagc tgtcggagag caggaagatc cggacgaagc tgctttcctc gctccagaag
      901 tcgctcgacg agcatacgcc ggaaaccgag gagcatatca gttccatgcg ccggattgcc
      961 cgaatcatga gcgaccttct gcagctctcc ccggaagagc gggagttgct tgacctggcc
     1021 gtccgtttcc acgacatcgg caaagcctcg attcccgtgt atctgctgaa taaaatcgcc
     1081 ccgatcaccg acgaagaccg cgaaacgctc cgcaggcatg cggagatcgg gggaaaaatc
     1141 gcggaggcgg cgacgtccga gacggcgaag tcggcgcccg ttattcgagc gcaccatgaa
     1201 cgatgggacg gcacaggcta cccctcgggg attgccggag aggatattcc cctgctctcc
     1261 cggatcattt ccattgccga cgcgttcgat gtcatgacca gaggaactgc ctacaaggac
     1321 gccgtttctc ccgaggaagc cctggaagag ctggagaggc tcgccgggct gcaattcgac
     1381 ccgcggcttg tcgatctctt cgcagaacat gcggaaaagt ttttgacgga tgaatctccg
     1441 gaaactcttc ccgaacgcag aaaatagggt agaataggtc tgaagccgtt gtttgcggca
     1501 tcttaatcag caaggaggga gttcatcatg ctcgatcttt ccgctattct gcagcccggc
     1561 ttggccggcg aagtgaaaaa gacggtggac ctcgacgata cgacgggaac ctcttctccg
     1621 tatctcggac agtatctttc aacttccgca tgcgcggcgc tcgcggtgca tgcatccatg
     1681 gcggcgaccg aaggacgtct gccggaaggg tacctctccg taggacgcag gctcgatctc
     1741 gagcacgatg cgcccgccat gaccggaacg accgtcattg taaaggcaac gctccgggag
     1801 atccgcggga accgccttat cttcgacatc cttggctccg acgccctcgg cgtcattttc
     1861 aagggcgtca acgaaagagt cgtcgtcaac cgtatcggcc ttgaggagcg aggaacggaa
     1921 cgcgcacaac agctgaaggc gctgcgcgaa aagctgtaag caagaggcgc ccggcgcgtt
     1981 cctccaagga acgccggata cgcggtctca ctgaac
//
LOCUS       DAOTIL010000520         2016 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminobacteriaceae bacterium isolate AMR_MDS_3026
            NODE_66341_length_2016_cov_2.904640, whole genome shotgun sequence.
ACCESSION   DAOTIL010000520 DAOTIL010000000
VERSION     DAOTIL010000520.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868050
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminobacteriaceae bacterium (wastewater metagenome)
  ORGANISM  Aminobacteriaceae bacterium
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae.
REFERENCE   1  (bases 1 to 2016)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2016)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:19:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,224
            CDSs (total)                      :: 3,198
            Genes (coding)                    :: 3,184
            CDSs (with protein)               :: 3,184
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2016
                     /organism="Aminobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_66341_length_2016_cov_2.904640"
                     /isolate="AMR_MDS_3026"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:3034609"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..677
                     /locus_tag="P5201_10285"
     CDS             <1..677
                     /locus_tag="P5201_10285"
                     /inference="COORDINATES: protein motif:HMM:NF013100.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="penicillin-binding transpeptidase
                     domain-containing protein"
                     /protein_id="HRV98971.1"
                     /translation="AKSSNTGMAQIGIRVKPFNMYHSVREWGFGKPTGIELPGAEDGL
                     LSSPEQWRGVVPSNIAIGQGLAITPLHLASAISAIANGGNLLRPHIVSKVVDSSGNIV
                     YNEERQFLRTVLSPQVALWLQRAMVDTVVKGTGRNGAVPGISIAAKTGTAQIAERGEY
                     AKGRWVSSFAGFWPSSDARYVVLVVIGEPSKGKYYGGDVAAPVFRRIVEDMEQIGLLA
                     AVKTEG"
     gene            685..>2016
                     /locus_tag="P5201_10290"
     CDS             685..>2016
                     /locus_tag="P5201_10290"
                     /EC_number="6.3.2.13"
                     /inference="COORDINATES: protein motif:HMM:NF001126.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanyl-D-glutamate--2,
                     6-diaminopimelate ligase"
                     /protein_id="HRV98972.1"
                     /translation="MILSKIIEEYAARYSSPLRLHNQAIDGGTDVSVVHVGCNSLEIK
                     KDELFCCIRGEHNDGHSFAGDAVQKGASTLLVERPLPLETPQIVSSNVRRDMGRLSSI
                     VYGDPANTLKMFAVTGTNGKSTTTWIIRHLLQSIGIRTGLLGTIVYNDGIEEKDAART
                     TPESCDIQLMLLRMLQNGCGACVMETSSHGLSFGRIEGCSFDAAVFTNLSEEHLDYHG
                     TLENYFSAKSRLFSDYMKQNWAGGANGDDAFARRLLGLYSDKLVSFGIEKEYGAHLLG
                     SNLQSSLEGTRFSLSLPGVSEPVPVFLPLPGKFNVYNALAALSATLPYATDKLALVDS
                     LHSMIQVPGRMERYSFNSGVSCIIDFAHTPFALQNVLTEVRRLCGGRIISVFGHGGER
                     FQPNRFSLGNIAATLADEVIVTMDNPRNEPAEQIAAQIVEGVESSERRVPLK"
BASE COUNT          513 a          458 c          533 g          512 t
ORIGIN      
        1 tcgcaaaatc ctcgaataca ggaatggcgc aaattgggat acgcgtaaaa ccattcaaca
       61 tgtatcattc agttcgcgag tggggattcg gcaaaccgac ggggattgaa cttcccggag
      121 ccgaggacgg ccttttatca tcaccggagc agtggagagg ggttgttcct tcgaatatag
      181 ccatagggca gggcctcgct attacgccgt tgcaccttgc atccgcaatc tctgcgatag
      241 cgaacggagg caatctcctg cgtccacata tcgtttccaa ggtagtagat tcttcaggaa
      301 atatagtata caatgaagag aggcagtttc ttcggactgt gttgtctcct caagtcgcgt
      361 tatggcttca gagagcgatg gtcgatactg ttgtcaaggg cacaggacgg aatggcgcag
      421 ttccaggaat ttcaatcgct gccaagacag gtactgctca gatcgctgaa agaggagaat
      481 acgccaaggg cagatgggtt tcctccttcg caggtttttg gccttcgagc gatgctcgct
      541 atgttgtgct tgttgtcatt ggagaacctt caaagggaaa gtattatggc ggagatgttg
      601 cagcgcctgt atttaggcga attgttgagg acatggaaca gatcggattg ttagcggcag
      661 tgaaaacgga aggatgatac cttgatgatt ctctcgaaga taattgaaga atatgccgca
      721 cgttattcat ctccgttacg gctgcataat caggcgatcg atggaggaac ggatgtttcc
      781 gttgtacatg tcggttgcaa cagcttggaa ataaagaaag acgagctgtt ttgctgcatt
      841 cgaggcgaac ataatgacgg ccattccttc gctggagacg ccgttcaaaa gggggcttca
      901 actcttttag tagagcgccc tcttccactg gaaacacctc aaatcgtgtc atccaacgtt
      961 cgaagagaca tgggacgttt atcttcaatt gtctacggag atccggccaa cactctgaaa
     1021 atgttcgctg ttaccggaac aaacggcaag agcacgacta cgtggataat acgccacctt
     1081 ctgcaatcaa taggaatacg gacaggcctt ttagggacaa tagtctacaa tgacggtatt
     1141 gaggaaaaag acgccgcaag aacaaccccg gaaagctgcg atattcagct gatgcttttg
     1201 agaatgctgc aaaatggttg cggtgcgtgt gttatggaga catcctcaca tggtctctct
     1261 ttcggaagaa ttgaagggtg ttcgtttgac gccgcagtct ttacaaacct cagcgaggag
     1321 catcttgatt accacggaac gctcgaaaat tacttttcgg caaagagccg tctgttttct
     1381 gactacatga agcagaattg ggcgggggga gcgaatggtg acgatgcatt tgcccgccgg
     1441 cttttaggcc tttattccga taagcttgtt tctttcggga tagagaaaga gtatggcgct
     1501 catctccttg gaagcaatct tcagagctct cttgaaggaa cacgtttttc gctttcactt
     1561 cctggagtca gtgaacccgt tcctgttttc ctgcctcttc cgggaaaatt caacgtgtac
     1621 aatgctttag ctgcgctctc tgcaacgctt ccatacgcta cggacaaatt ggctctcgtt
     1681 gattccttac attcaatgat tcaggttccc ggaagaatgg aaaggtattc cttcaatagt
     1741 ggagtgagtt gtatcatcga cttcgcccat actcctttcg cgctacagaa cgtactgacg
     1801 gaagtgcgtc gtctctgcgg aggaaggatt atttccgtat tcggacacgg cggagaacgt
     1861 ttccagccga acaggttttc attggggaat atcgccgcta cgcttgcgga tgaagtgatc
     1921 gtcacgatgg acaacccgcg gaacgaaccg gcggagcaga tagccgccca aatagtggaa
     1981 ggcgtcgaat cctccgaaag aagggttccc ttgaaa
//
LOCUS       DAOTIL010000521         2008 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminobacteriaceae bacterium isolate AMR_MDS_3026
            NODE_66692_length_2008_cov_3.932924, whole genome shotgun sequence.
ACCESSION   DAOTIL010000521 DAOTIL010000000
VERSION     DAOTIL010000521.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868050
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminobacteriaceae bacterium (wastewater metagenome)
  ORGANISM  Aminobacteriaceae bacterium
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae.
REFERENCE   1  (bases 1 to 2008)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2008)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:19:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,224
            CDSs (total)                      :: 3,198
            Genes (coding)                    :: 3,184
            CDSs (with protein)               :: 3,184
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2008
                     /organism="Aminobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_66692_length_2008_cov_3.932924"
                     /isolate="AMR_MDS_3026"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:3034609"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..599
                     /locus_tag="P5201_10295"
     CDS             <1..599
                     /locus_tag="P5201_10295"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: protein motif:HMM:NF013700.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="M23 family metallopeptidase"
                     /protein_id="HRV98973.1"
                     /translation="TYRQEIASSDSFLQKVRSERALHQRAVKELQDSQREIQSTIMDL
                     MRKKREAEMRPDAPPVTREEVPSGGKLAWPLPSKGRITSSFGMRVHPTFKTRMMHTGI
                     DIQMPSGTPVYAAGPGEVLYAGWLRGYGQIVIIDHGRDLSSVYAHLSRLAVGEGDRVK
                     KGQNIGAVGTTGTTTGAHLHFEVRVNGEAKDPMKYLGK"
     gene            624..1880
                     /locus_tag="P5201_10300"
     CDS             624..1880
                     /locus_tag="P5201_10300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015557091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S41 family peptidase"
                     /protein_id="HRV98974.1"
                     /translation="MHKELSPLWRRVRGWIAGMVVGVVVGVAVTGGLLAAGAMDLGEF
                     ARISPFNARAMWLMKQARAIIETYQVDSGTKTVEEDDLLYGAIHGMVAAWGDPYTRFV
                     DPSQLKEEEIEMEGQYGGLGIYIGQRDGRTLVISPIEDTPADRAGLKPQDQIVKIEDE
                     IILGWDSQKVVKHLRGAPGTSVTIWVRREGEEELLKFELAREIIKLKSVRSEMMEETL
                     GYIKLSQFKQNTADEFESALRELIGDGAKGLILDLRNNGGGLLNGAMEICDMFLDGGL
                     VVGMKGRVERANDEMFASSGTLTDLPMVVLINEGSASASEIVAGALADRGRALSVGKK
                     SFGKGSVQTLFNLGDGSGIYVTIARYQTPSGKVIDNIGLEPDIDVEGEVTKEAEKDEQ
                     LKRAKLELRDLISGKRATVPKVPSDF"
BASE COUNT          469 a          498 c          672 g          369 t
ORIGIN      
        1 agacttaccg gcaggagatt gcgagcagcg acagcttcct tcaaaaagtc cggagcgagc
       61 gcgccctcca tcagcgggcc gtcaaggagc ttcaggactc tcagcgggag attcagtcca
      121 ccatcatgga tctgatgcgg aagaagcgcg aggcggagat gcgtcccgac gcgcctccgg
      181 ttactcgcga agaggtcccg tcaggaggga aacttgcctg gccgctcccc tcgaagggac
      241 ggatcaccag ttcttttggt atgcgagtgc acccgacctt caagacgcga atgatgcaca
      301 ccggaatcga tatacaaatg ccaagcggaa ctccggtcta cgcggcgggt ccaggagagg
      361 tcctctacgc gggatggctt cgcggctacg gccagatcgt catcatcgat cacggcaggg
      421 atctctccag cgtgtacgcg cacctctctc gccttgcggt cggcgaaggg gaccgtgtga
      481 aaaaagggca gaatatcggc gcagtcggca ctaccgggac gacgacggga gcgcatcttc
      541 atttcgaggt cagagtcaac ggcgaagcca aggatccgat gaagtatctc gggaaataaa
      601 ccgtaaaaca aaggagaaga agaatgcata aggaactatc gccgctctgg aggcgggtac
      661 gaggctggat cgccggaatg gtcgtcggcg ttgtcgttgg agtcgccgtg acgggaggct
      721 tgctcgccgc aggagcgatg gatctgggcg agttcgccag aatatcaccg ttcaacgcac
      781 gcgcgatgtg gctgatgaag caagctcgag caatcataga aacatatcag gtagattcag
      841 gaacgaagac ggtcgaagag gacgacctgc tgtacggagc cattcacgga atggtcgcgg
      901 cgtggggcga cccgtatacg aggtttgtcg acccctctca gcttaaggaa gaagagatcg
      961 agatggaggg gcagtacgga ggtcttggca tctatatcgg acagcgcgac ggtaggacgc
     1021 tcgtcatcag tccgattgaa gatactccgg cggaccgggc cggcctcaaa ccccaggatc
     1081 agatcgtgaa aatcgaggat gagatcatcc tcggatggga ttcgcagaag gtcgtgaagc
     1141 atctgcgcgg tgctccggga acgtccgtca ccatttgggt tcgtagggag ggggaggagg
     1201 agctcctgaa gttcgagctc gcccgcgaaa taatcaagct gaagtccgtc cggtcggaga
     1261 tgatggagga aacgctgggg tatatcaagc tctcacagtt taagcagaat accgcggacg
     1321 agtttgaaag cgcgcttcgc gagctcattg gcgatggcgc gaaagggttg attctcgacc
     1381 tccggaacaa cgggggcggt ttgttgaacg gcgcgatgga gatctgcgac atgttcctcg
     1441 acggaggcct cgtcgtcggg atgaaggggc gcgtcgagag ggcgaacgat gagatgttcg
     1501 ccagcagcgg gacgctgacc gatcttccga tggtcgtcct gatcaacgag gggagcgcgt
     1561 ccgcctccga aatagtcgcg ggagctctcg cggacagggg gcgcgcgttg tccgtaggaa
     1621 agaaaagctt cgggaagggc tcggtgcaga cgctcttcaa tctgggagac ggctccggaa
     1681 tatatgtcac catcgcacgg tatcagaccc cttcgggaaa ggtgatcgac aatatcggcc
     1741 tggaaccgga tattgatgtg gagggggaag taacgaagga agcggaaaaa gatgaacagc
     1801 tcaaacgggc gaagctggag ctgcgggact tgatttccgg aaagagggcg accgtgccga
     1861 aagtaccatc tgatttttga gcaatcttgg cgcctgatgt cggttgatga agagaggacg
     1921 gtatgcaacg gccttgagga gtgtgcgctt cattctcgtg atcttggcgg cgctgctctc
     1981 tggagagtgt gctgcgctgc ctggaagc
//
LOCUS       DAOTIL010000522         2005 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminobacteriaceae bacterium isolate AMR_MDS_3026
            NODE_66820_length_2005_cov_4.273333, whole genome shotgun sequence.
ACCESSION   DAOTIL010000522 DAOTIL010000000
VERSION     DAOTIL010000522.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868050
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminobacteriaceae bacterium (wastewater metagenome)
  ORGANISM  Aminobacteriaceae bacterium
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae.
REFERENCE   1  (bases 1 to 2005)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2005)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:19:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,224
            CDSs (total)                      :: 3,198
            Genes (coding)                    :: 3,184
            CDSs (with protein)               :: 3,184
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2005
                     /organism="Aminobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_66820_length_2005_cov_4.273333"
                     /isolate="AMR_MDS_3026"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:3034609"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            132..899
                     /locus_tag="P5201_10305"
     CDS             132..899
                     /locus_tag="P5201_10305"
                     /inference="COORDINATES: protein motif:HMM:NF018290.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HRV98975.1"
                     /translation="MKIVGFCNLKGGVGKTTACQNLAVALVRRGLKVAALDLDPQSNL
                     TAGFGISIPEDRPYIYDFLMGDVSFGDLKTVREGVDVAPSTLDLAVVEMQLESQPGRD
                     TLLKDALASSDLSEYDVVFCDSPPQLGIFTRNVLAACGEILVPLESEFYSLAGLRLLS
                     QTVELYKKRLNRGLQISGILLTRHNPKIIMNREVEKEVRSHFGDTVFRRYIRQNISLV
                     EASGAGLSVFGYDEESNGARDYGVLADELLERWKKNG"
     gene            892..1458
                     /locus_tag="P5201_10310"
     CDS             892..1458
                     /locus_tag="P5201_10310"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRV98976.1"
                     /translation="MAKKKRPSLKDYLSTGQVIAEEVQEQSVSLEEAPAAPPAGEPSA
                     PEKKPAKARKPRSSAAKIGAAKKKSPVPDESAGPSTGESSSPDEGSVVLQEPSGELRS
                     FVDMLCGEDRKTWEKIFAVTGAEFLPLDFVERREDFRTMDRSRFTLFKLEEAGKPLFH
                     VRTSVQIREPLIALLKWDETGKATIYRP"
     gene            1704..>2005
                     /locus_tag="P5201_10315"
     CDS             1704..>2005
                     /locus_tag="P5201_10315"
                     /inference="COORDINATES: protein motif:HMM:NF014689.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="manganese efflux pump"
                     /protein_id="HRV98977.1"
                     /translation="MSFVEMLLSAASLSMDAFAASLGIGACLGVHSFAAAFRVGAACG
                     TFQFVMPLAGWWLGSRFLESISEYDHWLAFALLLLVGGGMIRESFSEEDSTCSPSDP"
BASE COUNT          423 a          538 c          605 g          439 t
ORIGIN      
        1 gctatgcaca ttactcttcg ggcgtcaatg tgacttttcg caacagccgt ttcttgctat
       61 gctatattgc gaggttatgt tatatactcg actggatgct ttgatcgcac atttgtccgg
      121 ggggattctt catgaaaatc gtcggttttt gcaatctcaa aggcggtgtc ggcaagacca
      181 ccgcgtgcca gaaccttgcg gtggctcttg tccgcagagg actgaaagta gccgcgctcg
      241 acctcgatcc gcagagcaat ctcaccgccg gcttcggcat ctctatcccg gaggaccgtc
      301 cgtatatcta cgacttcctt atgggagacg tttctttcgg ggacctgaaa actgtccgcg
      361 aaggagtgga tgtggcgccg agcacgctgg acctcgctgt ggtggagatg cagctcgaaa
      421 gccagccggg acgcgatacg ctgctgaagg acgccctcgc ctcgtccgac ctttcggagt
      481 acgacgtcgt cttctgcgac agccccccgc aactcggcat tttcacacgg aacgtccttg
      541 ccgcctgcgg ggagatactt gtcccgcttg agagcgagtt ttacagcctc gcaggactcc
      601 gtcttctctc gcagacggtg gaactctaca aaaagcgcct gaaccggggg cttcaaatca
      661 gcggaatact tctgacgagg cacaacccga aaatcatcat gaaccgcgaa gtggaaaaag
      721 aagtccgcag tcatttcggc gacacggtct tccggcgtta catccggcag aacatcagtc
      781 tcgtggaggc cagcggagcg gggctctccg tcttcggata cgacgaggag agcaacggcg
      841 ccagagacta cggcgtactg gccgacgagc ttctggagag gtggaagaaa aatggctaag
      901 aaaaagagac cgagtttgaa ggactacctg tcgaccgggc aggtgattgc cgaggaggta
      961 caggaacagt ctgtttcgct cgaagaggcg cctgcggctc ctccggccgg cgagccgtcc
     1021 gcgccggaaa agaaacccgc caaggcgcga aaaccgcgga gcagcgcggc gaagatcggc
     1081 gcggcgaaga aaaaatctcc cgttccggat gaaagcgccg gcccgtccac cggagaaagt
     1141 tcgtctcccg atgagggaag cgttgtactg caagagccaa gcggcgagct tcgctcgttc
     1201 gtcgacatgc tctgcggcga ggaccgaaaa acgtgggaga agatctttgc ggttactgga
     1261 gcggagttcc tgccgcttga tttcgtcgaa cgcagggagg attttcgtac gatggacagg
     1321 agccgtttta cgctcttcaa gctggaagag gcagggaagc cgctcttcca cgttcgcacc
     1381 agcgtgcaga tccgcgaacc gctgatcgcg ttgctgaagt gggacgaaac gggaaaggcg
     1441 accatttata ggccgtaaac aggctgtacc ctgtgaaagg tgttgtaggc gttcgggagt
     1501 cagagagaac gctctgttct gcggcgtact gcaaccaacg gtgcgacgaa ggaaaagcat
     1561 ctgtcgcgtg cggctgagaa tggagctgcc gcacgccatt gatcgcgttt caagaatcgg
     1621 ctctgtgaaa ataaaaaaac cggccttcga catcttgtgc gtatggagaa ggtctttttt
     1681 tatgaagaaa ggtatgatgt ttcgtgtcgt tcgttgaaat gcttctttcc gccgcgtcgc
     1741 tgtcaatgga cgcttttgca gcttccctcg gtatcggcgc ttgcttgggt gtacactcgt
     1801 ttgcagctgc attccgcgtc ggtgcggcgt gcggaacctt tcagttcgtt atgccgctcg
     1861 ccggctggtg gctggggagc cgttttctgg agtcgatctc cgaatacgac cactggctgg
     1921 cttttgccct ccttcttctt gtagggggcg ggatgatacg ggagtccttc tccgaagagg
     1981 attctacctg ctctccttcg gaccc
//
LOCUS       DAOTIL010000523         2005 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminobacteriaceae bacterium isolate AMR_MDS_3026
            NODE_66840_length_2005_cov_2.873333, whole genome shotgun sequence.
ACCESSION   DAOTIL010000523 DAOTIL010000000
VERSION     DAOTIL010000523.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868050
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminobacteriaceae bacterium (wastewater metagenome)
  ORGANISM  Aminobacteriaceae bacterium
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae.
REFERENCE   1  (bases 1 to 2005)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2005)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:19:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,224
            CDSs (total)                      :: 3,198
            Genes (coding)                    :: 3,184
            CDSs (with protein)               :: 3,184
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2005
                     /organism="Aminobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_66840_length_2005_cov_2.873333"
                     /isolate="AMR_MDS_3026"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:3034609"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..223
                     /locus_tag="P5201_10320"
     CDS             <1..223
                     /locus_tag="P5201_10320"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRV98978.1"
                     /translation="LGYLKAVLPGYADEEFYVDRPAVADGNLITAGGLDRIEFAYEIV
                     KRLEIFDEAEAGELYEMFKNGVVPARYMA"
     gene            complement(319..1047)
                     /locus_tag="P5201_10325"
     CDS             complement(319..1047)
                     /locus_tag="P5201_10325"
                     /inference="COORDINATES: protein motif:HMM:NF015716.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000902 - cell morphogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="septum site-determining protein MinC"
                     /protein_id="HRV98979.1"
                     /translation="MFNILETREKLLKKFVEQGNEPRILLKGAGYGIRVILQDDAAED
                     ELLEEARASVEELARIAGDTGLVIDCKMRKLTPDFLSRLLCDFVWAGGFRVLSWTSDD
                     TETLELFRNTGFTTGEPVSGRPDRERVESGSLILTQSLRSGQMVEHDGDVMLLGHMNE
                     GAEIRASGNIFVHGRLKGVVHAGTRGGEHGVWAERFEATQVRIGGKICSSLGRESAWW
                     GKAVLIALENGQLVARELKLDFAS"
     gene            1289..>2005
                     /locus_tag="P5201_10330"
     CDS             1289..>2005
                     /locus_tag="P5201_10330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012870302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein"
                     /protein_id="HRV98980.1"
                     /translation="MEVQNNEWQVVVFFLGGQAFAISVDKTREILRWPGSRPIPESHP
                     AMIGITSVRGEVMPLLDLRAYLGITPKVPLESSKVIIAEFNESKLGFVVDGVERIYGI
                     SAAELDSTLTSAVLGKSILYVIKRNESNVLIPDYEAIIQAAAPSLDRSLSIDLKKVAE
                     LAAPLGDLDRYRILVAEDSPLIRTQISDVLTQGGFHNVTLVADGKEAWERLAMKNERY
                     DLLVTDVEMPRLDGLALVRRV"
BASE COUNT          387 a          630 c          596 g          392 t
ORIGIN      
        1 cctcggctac ctcaaggcag tgctgcccgg atacgccgac gaggagttct acgtggaccg
       61 gccggcggtc gccgacggaa atctgataac agcgggaggg ctcgacagga tcgagttcgc
      121 gtacgaaatc gtcaagcggc tggaaatctt cgacgaagcc gaggcgggcg agctgtacga
      181 gatgttcaaa aacggcgtcg tcccggcaag gtatatggcg taacatgcga aagggagagc
      241 gcggcggccg ttgcgctctc cccttcatgg agttccgcga cgccgcagag cgccttcagg
      301 ggcgcgggcg tctttttttt atgacgcgaa gtcgagtttc agctcccgtg cgaccagctg
      361 cccgttctcc agcgcgatca gcactgcctt tccccaccag gcgctctccc tgccgaggga
      421 ggaacagatc ttccccccga tgcgcacctg cgtcgcctcg aagcgctccg cccacacgcc
      481 gtgctcgccg ccgcgcgttc ccgcgtggac tacgcccttg agcctgccgt ggacgaagat
      541 gttcccggac gcacggattt ccgcgccctc gttcatgtgc cccagaagca tgacgtcgcc
      601 gtcgtgctcc accatctgcc ccgagcgaag cgattgcgtc aggatgagcg agcccgattc
      661 gacgcgctct ctgtccgggc gtccggagac cggctctccg gtggtgaacc ccgtattgcg
      721 aaagagctcc agcgtctccg tatcgtccga cgtccatgaa agaacgcgga aacctccggc
      781 ccatacgaag tcgcagagca gtcgggaaag gaaatccggc gtgagtttgc gcatcttgca
      841 gtcgatcacc aggccggtgt cgccggcgat gcgggccagt tcttcgacgg aggcgcgcgc
      901 ctcctccagc agttcgtcct ccgcggcgtc gtcctgcaat atgacgcgaa tgccgtaccc
      961 ggctcccttg aggagaatcc gcggttcgtt cccctgctcg acgaacttct tcaacagctt
     1021 ttccctggtt tccaggatat tgaacatggc gttcactccc tttcgcgttc tccccggtga
     1081 tttctctctt gagaaacagc ctacaccttg acatagtgtg agagtcaagc ggaggagcgc
     1141 aatcttttga ttccagagcg aatttttaaa tgtgggagcg gcgcgtaacg ggcgaccttc
     1201 gctcacggga cttgtggtcc ggggctgaat ggcatatcat attgtaatga actgagagat
     1261 tccgttccgg ccagaggagg gagcgttcat ggaagtacag aacaacgagt ggcaggtagt
     1321 ggtcttcttt ttgggagggc aggcgttcgc catcagcgtg gacaagacca gggaaattct
     1381 tcgctggccg ggcagccgtc ctattccgga gtcccacccc gccatgatcg gcatcacatc
     1441 cgtccgcgga gaggtaatgc ccctgcttga cctcagggcg tacctcggca tcacgccgaa
     1501 agtcccgctg gaatcgagca aagtgatcat cgcggagttc aacgagagta aactggggtt
     1561 cgtcgtcgac ggcgtcgaac gcatttacgg aatcagcgcc gcggagttgg attccacatt
     1621 gacgagcgcg gtgctcggca agtccatcct ctacgtcatc aagcgaaacg agagcaacgt
     1681 cctgatcccc gattacgagg cgatcatcca ggcggccgct ccttccctcg acagaagcct
     1741 ctccatcgac ctgaagaagg tcgcggaact cgccgcgcct ctcggagacc ttgaccggta
     1801 ccgtatcctc gtcgcagagg actcgccgct tatccggacg cagatatccg atgtgctcac
     1861 ccagggcggc ttccataacg tgacgctcgt cgccgacggc aaagaggcct gggagcgtct
     1921 ggcgatgaag aacgaacgct acgatctcct cgtcaccgac gtcgaaatgc cccgcctcga
     1981 cgggcttgcg ctcgtccgcc gcgtc
//
LOCUS       DAOTIL010000524         1996 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminobacteriaceae bacterium isolate AMR_MDS_3026
            NODE_67221_length_1996_cov_3.640392, whole genome shotgun sequence.
ACCESSION   DAOTIL010000524 DAOTIL010000000
VERSION     DAOTIL010000524.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868050
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminobacteriaceae bacterium (wastewater metagenome)
  ORGANISM  Aminobacteriaceae bacterium
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae.
REFERENCE   1  (bases 1 to 1996)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1996)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:19:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,224
            CDSs (total)                      :: 3,198
            Genes (coding)                    :: 3,184
            CDSs (with protein)               :: 3,184
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1996
                     /organism="Aminobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_67221_length_1996_cov_3.640392"
                     /isolate="AMR_MDS_3026"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:3034609"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..613)
                     /locus_tag="P5201_10335"
     CDS             complement(<1..613)
                     /locus_tag="P5201_10335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006582741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class III-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HRV98981.1"
                     /translation="MMNSAELFERAKRSLVGGVNSPVRSWRGVGGTPLFFQRGEGPFL
                     HSVDGGRYTDYVCSWGPLILGHAHPEVVEAVCVAARESTSFGAPCPKEVELAEEVRRV
                     FPSMDLVRFVSSGTEAVMTALRLARGFTGREIVLKFDGCYHGHSDGMLVSAGSGALTL
                     GKPDSGGVPESVASTTLVVPYNDLERVSEAFDRFGDRIAAVIVE"
     gene            complement(642..1727)
                     /locus_tag="P5201_10340"
     CDS             complement(642..1727)
                     /locus_tag="P5201_10340"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRV98982.1"
                     /translation="MVGATDWRGLLYGELLLVVTVTAGLGFGKLLLAASVPERVMRYL
                     LPGADARRGRGGLHPDLLFALAVSLGSSRAGAALVAEAQREGRLSRREAVFGTLLQSF
                     PGYIKRWLISFPTAFGLAGAAGAIYSLIVLLRSFCRFLFFLALLRKGPSHDLPQGAAS
                     GVSHPPFVPGESATLPGAEPGREDGASAKIRDSILPQQTHEREAVAQGPAGGVHVSRG
                     RALPLFRTLARTLPLACFVYALAYLLSPSIQAFLQEKGASFPLLSSAGWTVAAASFAH
                     VNAALGVAGGAIASGALSTAQGVLALLVGNMLAVLSRVLRQDLAFWLGIFPGRMVRSL
                     FVWNLLTLVVTMAVTVALAAIPVLRGW"
BASE COUNT          395 a          710 c          626 g          265 t
ORIGIN      
        1 gttcgacgat cacggcggcg atacggtcgc cgaaacggtc gaacgcctcg ctcacccgct
       61 caagatcgtt gtacggaacg acgagcgtcg tcgaggccac gctctccgga acaccgccgc
      121 tgtcgggttt ccccagggtg agcgcgccgc tcccggcgct caccagcatg ccgtcggagt
      181 gcccgtggta gcagccgtcg aacttcagca cgatctccct cccagtgaaa ccgcgcgcca
      241 ggcggagcgc cgtcatcacc gcctcggtcc cggaggagac gaaacgtacc aaatccatcg
      301 aggggaagac gcgccgcacc tcctcggcaa gttcgacctc ttttgggcag ggcgcgccga
      361 aggaggtcga ctcccgcgcg gcaacgcaga cagcctcgac aacttccgga tgggcgtgtc
      421 cgaggatcag cggcccccag ctgcacacat agtccgtata ccgcccgccg tcgacggagt
      481 ggagaaacgg cccctcgccc ctctgaaaaa agagcggcgt tccgccgact ccgcgccacg
      541 agcgcaccgg actgttcacg ccgccgacaa gcgagcgttt cgcccgctcg aagagttccg
      601 cgctgttcat catttcaaaa acctcccctg aaacaccata tctaccaccc tctgagcacc
      661 gggatggcgg ccaatgcgac agtcacggcc atcgtgacga cgagcgtgag cagattccac
      721 acgaagagcg agcggaccat gcgccccgga aagatcccga gccagaaggc gagatcctgc
      781 cggagcaccc tggagagcac cgcgagcata ttcccgacga gcagcgcgag caccccctgc
      841 gccgtggaga gcgctcccga ggcaatcgcg cctcccgcaa ccccgagggc cgcgttgacg
      901 tgcgcgaagg acgcggcggc gaccgtccat cctgcgctcg acaacagggg aaaggaggct
      961 cctttctcct gaagaaaagc ctggatcgac ggcgaaagca gatacgccag cgcatagacg
     1021 aaacaggcga gcgggagggt gcgcgccaac gtccggaaga gcggaagagc gcgcccccgg
     1081 ctgacgtgca cgcctccggc cggtccctga gcaaccgctt ctctttcgtg cgtctgctgc
     1141 gggagaatgc tgtcccggat ctttgcagag gctccgtcct ctctccccgg ctccgccccg
     1201 gggagcgtag cagattcacc cgggacgaag ggggggtgcg aaacacccga cgccgctcct
     1261 tgcggcagat cgtgagacgg gcctttccgc agaagcgcga ggaagaagag aaaacggcag
     1321 aagctgcgca gcagcacgat gagcgagtag atcgccccgg cggctcccgc gagaccgaac
     1381 gcggtgggga aggagatgag ccagcgtttg atgtaccccg gaaacgattg aagcagcgtc
     1441 ccgaagactg cttcacgccg cgaaagcctg ccctcccgct gcgcctccgc cacgagcgcc
     1501 gccccggccc tcgacgaccc gaggctcacc gccagagcga agagaaggtc cgggtgcagc
     1561 cctccgcgtc cgcgccgagc gtccgctccc ggcaaaagat atcgcatcac gcgctccggc
     1621 accgaagcgg cgaggagcag cttgccgaac ccaagtccgg cggtcacggt gacgacgagc
     1681 agcagctcgc cgtagagcag tccgcgccag tccgtcgcgc ctaccatggc gcacgccttc
     1741 tctgctgccg tctccgcgaa aacggcgtac acaggaagct ccgcatgtgc gccgcccgga
     1801 tgcagctatc cgcgcgttcc tcgtcgcggc ggccacgtcc gttccccgcg gcgaagcggg
     1861 cggcgtgcgg acaaggggtc agcgccatcg caaaagctgc accacgctca gatactcgga
     1921 ggatggaacg agcgcctctt ccccctccac aatccgctcg tcgggaagcc ccgccctgtc
     1981 gatccggagc gcggtt
//
LOCUS       DAOTIL010000525         1993 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminobacteriaceae bacterium isolate AMR_MDS_3026
            NODE_67361_length_1993_cov_4.774510, whole genome shotgun sequence.
ACCESSION   DAOTIL010000525 DAOTIL010000000
VERSION     DAOTIL010000525.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868050
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminobacteriaceae bacterium (wastewater metagenome)
  ORGANISM  Aminobacteriaceae bacterium
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae.
REFERENCE   1  (bases 1 to 1993)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1993)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:19:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,224
            CDSs (total)                      :: 3,198
            Genes (coding)                    :: 3,184
            CDSs (with protein)               :: 3,184
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1993
                     /organism="Aminobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_67361_length_1993_cov_4.774510"
                     /isolate="AMR_MDS_3026"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:3034609"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(294..613)
                     /locus_tag="P5201_10345"
                     /pseudo
     CDS             complement(294..613)
                     /locus_tag="P5201_10345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611505.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - transposition, DNA-mediated
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(816..1568)
                     /locus_tag="P5201_10350"
     CDS             complement(816..1568)
                     /locus_tag="P5201_10350"
                     /inference="COORDINATES: protein motif:HMM:NF012514.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HRV98983.1"
                     /translation="MELLEIRNADGSGTGQYKDASEIHRDGNLHGRVQIWFLSEIKES
                     GNFSLLIRKRSAESSESPGLFTALSTGHVAPGETFEATAKRLLVEDLEMVPPKEFAFQ
                     FDYPLVHESEINGQQAIDREFCRVFLLTNEALPDPCPSSRRPDLFAWCDAQSLLSSLR
                     EGDPRYSIDCSLCEKIIDTYAGTRQYTIHITDCWSAPSSGGYAGSDEWTYDQCYSFFG
                     TKEEAMKEGQWYVRAFHDGTSPYLASVIYWLT"
     gene            complement(1746..>1993)
                     /locus_tag="P5201_10355"
     CDS             complement(1746..>1993)
                     /locus_tag="P5201_10355"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRV98984.1"
                     /translation="FLCQYKNGTLSKLNVPGEVRIKDDWIDSIGEPVSRTYVGRFWRF
                     SDIRREVETHPEHANDGLKRVLEQLSENPDLFSALSV"
BASE COUNT          399 a          595 c          496 g          503 t
ORIGIN      
        1 gctgccgcca gccccttccc gggcgctctg gagccgccga ttttcttgcg gccagtcgat
       61 tagtccttct tccctgtaca gccggcagac ccgcttgtgg ttgattttcc atccttcgcg
      121 cagcaacagg acatgaatcc ttcgatatcc atatcgtttc ctcacagagg cgatctccct
      181 gatccgcatt cgtaaaaagg cctgcgcgtt ccggcttggc tgcctccggt gccatccccg
      241 attaccgtgc agcttctgcg ttcgctcgcc tggaagcgtt cctgcagaac ccgtacggcg
      301 tcccgtttcc cggcaggcgt tcggattttt ttgtcagtac gtcctgcgcg attcgcttat
      361 ctaaacgcaa gtctgccatc agtttcttca gctttgcgtt ctcttcttcc agctgccgta
      421 gccgcttcct atcgctcggc agcatcccgc cgtacttgct cttccagcga taaaacgtct
      481 gttccgcaac cccgagcttg cggcacacct ccgatatcgg cgtgcccagt tccgcctgct
      541 tcaatgcaaa ggcgatctgc tccttaggtg tatttgctct tcttcctttc gtccgctttt
      601 tcctttcccg cagtttatca ttccccctcc cttttggtgg actacttttt tagggagcag
      661 gtcaaatttc aggcagcgat tggaaccacg gtttgagggg cgggcggccg ccgccgaggc
      721 tcatacaaat ctttgacggc gaagagaaac aacgcgcctt ctttttttga tataagtcat
      781 atccgtacac ccttaagaag acggtaatcg cgcgtttacg tgagccaata gatcacactc
      841 gcaagatagg ggctggtgcc gtcgtgaaac gctcttacat accactgccc ttccttcatg
      901 gcctcttctt tcgttccgaa gaacgagtag cattgatcat aggtccactc atcggaaccg
      961 gcatagccgc ccgagctcgg agcggaccaa cagtcggtga tatggatcgt gtactgacgt
     1021 gtccctgcgt atgtgtcgat tattttttca cagagagaac agtcgatcga atagcgcggg
     1081 tctccctccc tgagtgaact gagcaaagac tgcgcgtcac accaagcaaa caggtccggc
     1141 cgcctactcg atggacaggg atcgggcaga gcttcattgg tcagcagaaa tacgcggcag
     1201 aattcgcgat cgatcgcctg ttggccgttg atttccgatt catgaacgag cggataatca
     1261 aattgaaacg cgaactcctt cggcggcacc atctccagat cctccacaag gaggcgtttc
     1321 gccgtcgctt caaaggtctc gccgggcgcg acatgccccg tgctgagggc ggtgaacaaa
     1381 ccgggagact cgctgctctc cgcgctgcgt tttcgtatca gcagggagaa gttcccgctt
     1441 tctttgattt cactcaaaaa ccagatctgc acacgcccgt gcagattacc gtcccgatgg
     1501 atctcggagg catctttata ctgcccggta ccgctcccgt ctgcgtttcg tatttccagc
     1561 aattccatat ggttttcctc cttttttgcg ggctggcgcc tgcggatgaa atgatcttgg
     1621 gtttcgagcg cgaccggtct ccggctaaag gaacgcggag caaacgacat gcggccaatc
     1681 caacgttcgg tatccgaata agaacctggc accgaatctt ctaccgagcc ccgtacacaa
     1741 tagctttaca cggaaagagc gctgaacaga tccggatttt ccgataattg ttccaggaca
     1801 cgcttcaacc cgtcgttagc gtgctcggga tgcgtctcga cttcgcggcg aatgtcgctg
     1861 aatctccaaa agcgccctac atacgtacgg gagaccggct cgccgatgga atcgatccag
     1921 tcgtccttga tacgcacttc accaggtacg ttaagcttgc ttaatgtgcc gtttttgtat
     1981 tggcagagaa aga
//
LOCUS       DAOTIL010000526         1991 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminobacteriaceae bacterium isolate AMR_MDS_3026
            NODE_67461_length_1991_cov_2.746901, whole genome shotgun sequence.
ACCESSION   DAOTIL010000526 DAOTIL010000000
VERSION     DAOTIL010000526.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868050
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminobacteriaceae bacterium (wastewater metagenome)
  ORGANISM  Aminobacteriaceae bacterium
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae.
REFERENCE   1  (bases 1 to 1991)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1991)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:19:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,224
            CDSs (total)                      :: 3,198
            Genes (coding)                    :: 3,184
            CDSs (with protein)               :: 3,184
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1991
                     /organism="Aminobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_67461_length_1991_cov_2.746901"
                     /isolate="AMR_MDS_3026"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:3034609"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1506)
                     /locus_tag="P5201_10360"
     CDS             complement(<1..1506)
                     /locus_tag="P5201_10360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009202127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter fused permease subunit"
                     /protein_id="HRV98985.1"
                     /translation="MTIENNPPMEQIPLQKEIIEGRKRELEGWQYWVVAGIALAASLF
                     HLYTAAFGLLSAMYQRGVHWMFMGSLIFLIYPATKNRPKNKIDFWDWILAGMVVVGCL
                     NILLNWEAIAMREGMPVASDIYLGIMMVILVIEAGRRAMGWPLPIVAIIATIYALFGP
                     YFPGVLGHGGFPLEELAPFQYLRTDGIFGVPLGVSASFIFLFVLFGAVLSVSGAGQFF
                     IDLSLALTGRSQGGPGKAAVVASGLMGTVSGSSVANAVTTGAFTIPLMKEAGYSATFS
                     GAVVAASSTGGQVMPPVMGAAAFIMAQFLGISYWDIVVAAALPATLYFISIIAMVHFR
                     AGRRGIKSLTEETAPKMGKVLKEGWHLLLPIITLVVFLAYGYSAVKAVFWSIIIMVGV
                     SWLGKAQYRMTPKRVLDALISGGMGAVEVAAACACSGLVIGVIGITGVGLAFSSFVIS
                     LSGGVLPLALFLTMIGSIILGMGVPTTAQYIITSTLAAPALFQMGVPLMSAH"
     gene            complement(1607..>1991)
                     /locus_tag="P5201_10365"
     CDS             complement(1607..>1991)
                     /locus_tag="P5201_10365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005659550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="TAXI family TRAP transporter solute-binding
                     subunit"
                     /protein_id="HRV98986.1"
                     /translation="NFAAPGSAVLEVAAQRDVVMIPLEEALVQKIIEANPFLIPYTIK
                     AGVYESIKEDIRTIADSNYLVVKNDMDEALSYNLVKTLIENKDKFMNITTQAAHFVAE
                     EASVGITEFASGAKKYFKEQGIDMK"
BASE COUNT          465 a          599 c          561 g          366 t
ORIGIN      
        1 atgcgccgac atgagcggaa cgcccatctg aaaaagcgcg ggagcagcca gcgtcgatgt
       61 gatgatgtac tgcgccgtgg tgggaacgcc catgccaaga atgatcgagc cgatcatcgt
      121 gaggaagaga gcgagcggaa gcacgccgcc ggagaggctg ataacaaagg aggagaacgc
      181 aagcccgact ccggtgatac cgatgacgcc gatgacaagc cccgaacatg cgcacgcggc
      241 cgcaacttcc accgccccca tgccgccgct gatcagagcg tcgagaacac gcttcggcgt
      301 catgcggtac tgcgccttgc cgagccagga aacgccgacc atgatgatga tcgaccagaa
      361 gaccgctttc accgcggaat agccgtatgc gagaaagacg acgagggtga tgatcggcag
      421 cagaagatgc cacccttcct tcagcacctt ccccatcttg ggagcggtct cttctgtgag
      481 actcttgatc cctcgccttc cggcacgaaa atggaccatc gcgatgatgg agatgaaata
      541 gagcgttgcg ggaagcgcag cagcaacgac gatatcccag taggaaatac ccagaaactg
      601 cgccatgata aacgccgcag cccccatgac cggcggcatg acctggccgc ccgtggagga
      661 cgccgccacg acggctcccg agaacgtcgc gctgtatccg gcctccttca tcaacggaat
      721 ggtgaaggcg cctgttgtta cggcatttgc aacggagctt ccggaaaccg ttcccatcag
      781 cccgctcgcc acgacggctg ctttgccagg ccctccctgg ctccggccgg tgagggcaag
      841 cgaaaggtca ataaagaact gccccgcgcc ggaaacgctc aggacagccc cgaagagaac
      901 gaagagaaag atgaacgaag ccgagacgcc gagcggcacc ccgaagatac cgtcggtgcg
      961 cagatactgg aagggagcaa gctcttccaa gggaaagcct ccgtgtccga gcacacccgg
     1021 gaagtagggg ccgaagagcg cgtagatcgt cgcgatgatc gccacgatgg gcagcggcca
     1081 ccccatagcg cggcggccgg cttcgatgac cagaatcacc atcatgattc ccaggtagat
     1141 atcggaagcg acgggcattc cctcacgcat ggcgatcgcc tcccagttca ggaggatgtt
     1201 caggcagccg acgactacca taccggccaa tatccagtcc cagaaatcga tcttgttctt
     1261 cggacgattt tttgtcgccg ggtagattaa gaaaataagg ctccccatga acatccagtg
     1321 gacgccacgc tgatacatcg cagagaggag gccgaaagcc gccgtgtaca ggtggaacag
     1381 cgacgccgcg agcgcgatgc ccgccaccac ccagtattgc cacccttcga gctccctctt
     1441 tcttccttcg attatttctt tctgcagagg aatctgttcc atgggcggat tattttcgat
     1501 cgtcatatag aaacctccag acggagtgaa attagaacct gtaaagttcc cggccgaccg
     1561 aaaacgaccg gccgggagaa aaactgctgc cgcgtgctat tcttttttat ttcatatcga
     1621 ttccctgttc tttgaaatac ttcttggcgc ccgaggcaaa ctccgtaata ccgacgctcg
     1681 cctcttcggc gacgaagtgg gcggcctgcg tggtgatgtt catgaacttg tccttatttt
     1741 cgataagcgt cttcaccagg ttgtatgaga gcgcctcgtc catatcgttc ttcaccacga
     1801 ggtagttgct gtcggcgatc gtccggatat cctccttgat gctctcgtag acgccggcct
     1861 tgatcgtgta cggaatcagg aaggggttcg cctcgatgat cttctgcaca agagcctcct
     1921 caagcggaat catcaccacg tcgcgctgcg cggccacttc aagcaccgcc gaaccggggg
     1981 ccgcgaagtt c
//
LOCUS       DAOTIL010000527         1983 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminobacteriaceae bacterium isolate AMR_MDS_3026
            NODE_67837_length_1983_cov_2.737033, whole genome shotgun sequence.
ACCESSION   DAOTIL010000527 DAOTIL010000000
VERSION     DAOTIL010000527.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868050
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminobacteriaceae bacterium (wastewater metagenome)
  ORGANISM  Aminobacteriaceae bacterium
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae.
REFERENCE   1  (bases 1 to 1983)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1983)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:19:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,224
            CDSs (total)                      :: 3,198
            Genes (coding)                    :: 3,184
            CDSs (with protein)               :: 3,184
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1983
                     /organism="Aminobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_67837_length_1983_cov_2.737033"
                     /isolate="AMR_MDS_3026"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:3034609"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..307
                     /locus_tag="P5201_10370"
     CDS             <1..307
                     /locus_tag="P5201_10370"
                     /inference="COORDINATES: protein motif:HMM:NF022077.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="P-loop NTPase"
                     /protein_id="HRV98987.1"
                     /translation="SPQELASLVVRKAAKMAEMLDIPLVGVVENMSSLRCPHCGERIE
                     LFGGSHAEALINDVKATFWGSLPLDTEMAALGDEGHLEEYTNAEVFSVLEKGLANLL"
     gene            451..984
                     /locus_tag="P5201_10375"
     CDS             451..984
                     /locus_tag="P5201_10375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012471001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_except=(pos:841..843,aa:Sec)
                     /transl_table=11
                     /product="YeeE/YedE thiosulfate transporter family
                     protein"
                     /protein_id="HRV98988.1"
                     /translation="MKEEGGWNPYLAGGLSGLVSIGSVWFAGKYLGASTTFVRATAAV
                     EQAVLPERAATLEYLVKTAPKMDWQAWFLVGIFIGSLVAAALFGDFKFELVPPMWKKR
                     FGDGVLKRGVVAFIGGVVAMFGARLADGUPSGHGLSGSLQLAVSGIVAFAAFYIAGMV
                     TAKLVYGGGERNGGSES"
     gene            965..1504
                     /locus_tag="P5201_10380"
     CDS             965..1504
                     /locus_tag="P5201_10380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013047924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YeeE/YedE thiosulfate transporter family
                     protein"
                     /protein_id="HRV98989.1"
                     /translation="MAGLNPDMVYGLVTGLLFGFLLQRGRVIRYDKQLGALRVQDFTI
                     LKFMLSSIVVAMVGVYILLDMGMVKLSVKPMIAGGVVVGGLLFGVGWGLLGYCPGTSV
                     AAVGEGRLDALWGIAGGLVGAALYAEAFPWVSRTLLTMGDYGKITIPQALGMNHWYVI
                     GGAAVLTLLFFMVSEKKGL"
     gene            1640..>1983
                     /locus_tag="P5201_10385"
     CDS             1640..>1983
                     /locus_tag="P5201_10385"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRV98990.1"
                     /translation="MGKKVREGVFVYSVVLVMLLFAAQAQAGGVRLSPLNPEFAGASA
                     LSAGASAGAAQIRTPWGDVLPVGRRPSPLNLEHLKHPEAGASARTKRALPAKYDLRTL
                     NRLPPIRDQGLYG"
BASE COUNT          330 a          517 c          692 g          444 t
ORIGIN      
        1 ctcgccgcag gagcttgcct ccctcgtggt ccgcaaggcc gcgaagatgg cggaaatgct
       61 ggatattccc ctcgtcggcg tggtggagaa catgagcagc cttcgctgcc cgcactgcgg
      121 cgagcggatc gagcttttcg gcggaagcca cgccgaggcg ctgatcaacg acgtaaaggc
      181 gaccttctgg gggagcctgc ctctggatac ggaaatggcc gcgctgggag acgaggggca
      241 cctcgaggag tacaccaacg ccgaagtctt ctccgtgttg gagaaggggc ttgccaacct
      301 gttgtagcgc tctctccggc aagaagagtg aagagccctc cggggctctt ttttttatgt
      361 gaaaggttga caataccata taaggggata taattaaatt gtaaggaagt gcagtatttg
      421 taatattatc tggagaatgg aggggatacg atgaaagaag agggaggatg gaacccctac
      481 ctcgccggag gattgtccgg gcttgtttcc ataggctcgg tctggttcgc cgggaagtat
      541 ctgggggcgt ccacaacctt tgtccgggcg accgcggcgg tggagcaggc ggtgcttccg
      601 gagcgggcgg cgacgcttga atacctggtg aagacggccc cgaagatgga ctggcaggcg
      661 tggttccttg tgggcatttt catcggttcg ctggttgcgg ccgcactttt tggcgacttc
      721 aagttcgagc tcgtcccgcc gatgtggaag aagcggtttg gtgacggcgt gttgaagcgc
      781 ggggttgtcg ccttcatcgg cggcgtcgtc gcgatgttcg gcgcgcgtct cgcggacggg
      841 tgacccagcg ggcacgggct gagcggttcg cttcagcttg cggtcagcgg catcgtcgcg
      901 tttgcggcgt tctacatcgc cggcatggtt acggcgaaac ttgtgtacgg aggaggcgag
      961 cgcaatggcg ggtctgaatc ctgatatggt ctacggcctc gtcacggggc ttctcttcgg
     1021 tttcctgctg cagagggggc gcgtgatccg gtacgacaag cagctcggcg cccttcgcgt
     1081 gcaggacttc accatattga agtttatgct gtcgtccatt gtggtcgcga tggtcggcgt
     1141 ctacatcctc cttgacatgg gaatggtgaa actttccgta aagccgatga tcgcgggcgg
     1201 cgtcgtggtc ggcggccttc tcttcggcgt cggctggggg ctgctgggtt actgccccgg
     1261 cacttccgtg gcggcggtcg gggaggggcg tctcgacgcc ctctggggga tcgcgggcgg
     1321 cctcgtcggc gcggcgcttt acgccgaggc cttcccgtgg gtgagccgga cgctcctcac
     1381 gatgggagac tacgggaaga tcaccattcc gcaggcgctg gggatgaatc actggtacgt
     1441 catcggcggt gcggcggttc tcacgcttct tttcttcatg gtctcggaga agaaggggct
     1501 atagaaagac gttttttgct ttgcaccccg agcggtctcc ggataaactt ttccggaggc
     1561 cgcttttttc attccctgcc ggccgaaaag agtgtatgct ttatgggtgt ggagttgaat
     1621 ttgaaaggac ggggagttta tggggaaaaa agtgcgtgaa ggggttttcg tgtattctgt
     1681 tgttctcgtc atgctgctct ttgcagcgca ggcgcaggcg ggaggagttc gcttgtcgcc
     1741 gctgaatccg gagtttgcag gggcgtccgc gctctccgcg ggagcctccg ccggggctgc
     1801 gcagattcgc acgccctggg gtgatgtgct gcctgttgga agacggccgt cgccgctgaa
     1861 cctggagcat ctgaagcatc ctgaggcagg ggcgtccgcg cggacgaaga gggctctccc
     1921 tgcgaagtat gacctgcgga cgctgaacag gcttcctccc atccgggatc aggggctcta
     1981 cgg
//
LOCUS       DAOTIL010000528         1981 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminobacteriaceae bacterium isolate AMR_MDS_3026
            NODE_67919_length_1981_cov_3.176532, whole genome shotgun sequence.
ACCESSION   DAOTIL010000528 DAOTIL010000000
VERSION     DAOTIL010000528.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868050
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminobacteriaceae bacterium (wastewater metagenome)
  ORGANISM  Aminobacteriaceae bacterium
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae.
REFERENCE   1  (bases 1 to 1981)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1981)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:19:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,224
            CDSs (total)                      :: 3,198
            Genes (coding)                    :: 3,184
            CDSs (with protein)               :: 3,184
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1981
                     /organism="Aminobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_67919_length_1981_cov_3.176532"
                     /isolate="AMR_MDS_3026"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:3034609"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1249)
                     /locus_tag="P5201_10390"
     CDS             complement(<1..1249)
                     /locus_tag="P5201_10390"
                     /inference="COORDINATES: protein motif:HMM:NF027890.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HRV98991.1"
                     /translation="MASRLTKTRTLAAVAVAALVGAGYFIWGAVPAKREQTEVARVAR
                     PVKTMAVRAAGGGGERIFPGKVLASQKVNLAFRVSGQIAELPAVKGTFVEPGTLLARL
                     DPRDFEVQLANAKSELGNAKARLDAMKAGARKEEIAMLTSKVNSARAQMNDALTTLDR
                     VEKLYKAGGFSKSEYDKARTASEVARATYQSASQELSAARTGSRPEDVAAMEFTIQGI
                     EGKVRTAENALADTELLAPFGGVVIDRYVDNNQSIQRDQSIVSLQDIGTLEVAISVPE
                     TLVLHTRKKDIKGIEARFASLPDKRFPLTYKEASAQADPQTQTYPVTFALPTPEDVTV
                     LPGMTVDVIIPRPAGDEGAEAEIPASAVLAGDGTEHFVWRVEGTDELRVKKIPVEVAG
                     YRGDLALVKGVAPGNASSRRASPC"
BASE COUNT          351 a          599 c          618 g          413 t
ORIGIN      
        1 agcatggaga cgcccgccgt gacgatgcgt taccgggggc cactcccttt acgagagcga
       61 ggtcgccccg gtaccccgcg acctcgaccg gaatcttctt aacgcgcagc tcgtccgttc
      121 cctcgacgcg ccagacgaag tgttcggttc catcccctgc caaaactgcg gaggcgggaa
      181 tttcggcctc cgctccctcg tcacccgcgg ggcgcgggat gataacgtcc accgtcatgc
      241 cggggaggac ggttacatcc tcgggcgtcg ggagcgcgaa ggtgaccgga taggtctgcg
      301 tctgcgggtc ggcctgcgcg gaggcttcct tgtaggtcag cgggaagcgc ttgtcgggca
      361 gcgaggcgaa gcgcgcttca attcccttga tatccttttt ccgcgtgtgg aggacgagcg
      421 tctccggcac ggagatggcg acttcgagcg tgccgatgtc ctgcaggctg acgatggact
      481 gatcgcgctg gatactctgg ttgttgtcca cgtagcggtc gatgacgacg ccgccgaagg
      541 gggccagaag ctccgtgtcg gcaagggcgt tctccgcagt gcggaccttc ccctcgatcc
      601 cctggatggt gaactccatg gcggcgacat cctcggggcg cgagccggtg cgggccgccg
      661 acagctcctg cgaggccgac tgataggtcg cgcgggctac ctcggaggcg gtgcgggcct
      721 tgtcgtactc cgatttggaa aagccccccg ccttgtacag cttctccacc cggtcaaggg
      781 tggtgagcgc gtcgttcatc tgggcgcggg cggagttcac cttcgaggtg agcatggcga
      841 tctcctcctt gcgggctccg gccttcatgg cgtccaggcg ggcctttgcg ttccccagct
      901 cgctcttcgc gttggcgagc tgcacctcga agtcgcgtgg gtcgagacgg gccagcagcg
      961 tgcccggctc aacaaacgtc cctttcaccg cgggcagctc cgcgatttga ccggagacgc
     1021 ggaaggccag gttcaccttc tgcgacgcga gcaccttgcc ggggaagatt cgctctcctc
     1081 cgccgcctgc ggcgcgaacc gccatcgtct tcaccggacg ggcgacgcgg gcgacctcgg
     1141 tctgctcgcg cttcgcgggg acggcgcccc agatgaaata ccccgcgccg accagcgcag
     1201 cgaccgcgac cgccgcgaga gtgcgcgttt ttgtgagtct gctcgccatt tttaaaccac
     1261 cctttcgcat caagaaatag tttataagaa aaagagaagc cctcttttcg ctattataac
     1321 cgacacacaa aaaacgggga ccatggtctt gtccccggaa agagcgtctt caacctttca
     1381 ctccgcagcg cggtgtattt tttcgcgtcg gcgtctactt gagaagatcc gccagtgttc
     1441 tgatccagcc cgacttcatt ttctccatca tccagtcctc gccgacgatt gtggcgacgt
     1501 atatccctcc ccggacgtcg gcgtatggtg tgcagactga aattcgagtt tgccgatgcg
     1561 cgtaatcgac tgtacaatgc agtcggagga gatgcgggat ggggagcggc gcagagtatt
     1621 cgggctttgg gttcggtgtt tcgacgcatc gggcagagac tggcggagac gggcggccga
     1681 tgaacctgcc gatgccttcg caacagattc acaaattctt tggtaatcag ggagagtgtt
     1741 ggtatgaatt cattgtatcc gtacaaaata atgggtttca tcttcttcac atgtatgctg
     1801 tttttctttt ttcccgggcg ttcggaatgc gtcgtgaaca gtcaaagcga gcccgaagga
     1861 tacccgatcg cctccgacgt gcagacaacg gttcagagga cgatcgtgcc gggggcgacc
     1921 ccgacgactg cgatcagtct ccgggagatt tccaaatacg aacagtacgg ctacggcgat
     1981 t
//
LOCUS       DAOTIL010000529         1975 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminobacteriaceae bacterium isolate AMR_MDS_3026
            NODE_68188_length_1975_cov_3.826042, whole genome shotgun sequence.
ACCESSION   DAOTIL010000529 DAOTIL010000000
VERSION     DAOTIL010000529.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868050
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminobacteriaceae bacterium (wastewater metagenome)
  ORGANISM  Aminobacteriaceae bacterium
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae.
REFERENCE   1  (bases 1 to 1975)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1975)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:19:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,224
            CDSs (total)                      :: 3,198
            Genes (coding)                    :: 3,184
            CDSs (with protein)               :: 3,184
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1975
                     /organism="Aminobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_68188_length_1975_cov_3.826042"
                     /isolate="AMR_MDS_3026"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:3034609"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..447
                     /locus_tag="P5201_10395"
     CDS             <1..447
                     /locus_tag="P5201_10395"
                     /EC_number="2.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016293398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acyltransferase"
                     /protein_id="HRV98992.1"
                     /translation="GINDGAAGVVVMSEQKAKELGVVPMAVFEGGASAGVDPCVMGIG
                     PAFSTRKLLERTGRSIAEFDLIEANEAFAAQALAVGKLLEWDWNRVNVNGGAIALGHP
                     VGASGCRILVTLLHEMRRRGVRNGLATLCVGGGMGVSTAVGLYDGR"
     gene            465..1184
                     /gene="fabG"
                     /locus_tag="P5201_10400"
     CDS             465..1184
                     /gene="fabG"
                     /locus_tag="P5201_10400"
                     /EC_number="1.1.1.100"
                     /inference="COORDINATES: protein motif:HMM:NF009466.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-ACP reductase FabG"
                     /protein_id="HRV98993.1"
                     /translation="MRLENKTAIVTGGARGIGQAMCELFASEGARVLAADMSELSYEN
                     PSVEGYRLDVSDPEACRACFEYAVGRYGKIDVLVNNAGITRDALTRKMTDEMWHKVIG
                     VNLSGVFNLGRLVGPHMQEHGGGSIVCISSVVGEFGNIGQANYAAAKAGMIGLAKTWA
                     KEFAMKGGNVRVNVIAPGYTMTDILSTVPQELLDKFAAMTMLGRLGRPEEIAKAALFL
                     ASDDASYVTGHVLSVNGGMRL"
     gene            1272..>1975
                     /locus_tag="P5201_10405"
     CDS             1272..>1975
                     /locus_tag="P5201_10405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016293395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HRV98994.1"
                     /translation="MNFELSKEHRMARDLFRRFAEKEARPLAQEVDENERFPSETVEK
                     MARSGFLGIPCAKEWGGQGCDTLTYVLCVEELSRVCATTGVVVSAHTSLACDPIEKYG
                     TEAQKRKYLPELLAGRKLGAFALTEPGAGTDASGQQTRAVLEGENYRLNGSKIFITNA
                     GQADVYIVFAMTDKSLGTKGISAFIVEKGFPGFSVGKKELKMGIRGSSTCELIFEDCV
                     VPKENLLGKEGKGFSIA"
BASE COUNT          376 a          573 c          717 g          309 t
ORIGIN      
        1 ggcatcaacg acggggcggc tggggtcgtc gtcatgagcg agcaaaaggc gaaggagctg
       61 ggcgtcgtgc cgatggcagt cttcgagggc ggcgcgtcgg cgggggtcga cccctgcgtc
      121 atggggatag gccccgcgtt cagcacgcga aagctgctgg agcggacggg gcggagcatc
      181 gcggagttcg atctgatcga ggcgaacgag gcgttcgcgg cccaggcgct cgccgtcgga
      241 aagctgctgg agtgggactg gaaccgcgtg aacgtgaacg gaggggccat cgccctcgga
      301 caccccgtgg gcgcttccgg ctgccggatc cttgtgacgc tgctccacga gatgcggcga
      361 agaggcgtcc gaaacgggct cgccacgctc tgcgtcggcg gcgggatggg cgtctcgacg
      421 gccgtcggac tttacgacgg ccggtgatgg aaggagatgt gcgaatgaga cttgagaata
      481 aaaccgccat cgtcaccggc ggggcgcgcg gcatcgggca ggcgatgtgc gaactgttcg
      541 cctcggaggg cgcgcgcgtc ctcgcggcgg acatgtcgga gctgtcgtat gagaatccgt
      601 cggtcgaggg gtacaggctc gacgtctccg acccggaagc gtgcagggcg tgcttcgagt
      661 acgcggtcgg gcggtacggc aaaatcgacg tcctcgtgaa caacgcggga atcacccgcg
      721 acgcgctgac gcggaagatg accgacgaga tgtggcacaa ggtcatcggc gtcaacctga
      781 gcggcgtctt caacctcgga cggctcgtcg ggccgcacat gcaggagcat gggggcggct
      841 ccatcgtctg catctcctcc gtcgtggggg agttcggcaa catcggacag gcgaactacg
      901 ccgcagccaa ggccggaatg atcggcctgg cgaagacctg ggccaaggag ttcgcgatga
      961 agggcggtaa cgtccgcgtg aacgtgatcg ctccggggta caccatgacc gacattctga
     1021 gcaccgtgcc gcaggagctg ctggacaagt tcgccgccat gaccatgctc ggacgcctcg
     1081 ggcggcctga ggagatcgcg aaggcggcgc tcttcctggc gagcgacgac gcatcctacg
     1141 tcaccgggca tgtgctcagc gtgaacggcg gcatgcggct gtaacgaacg aatctattca
     1201 accacccgcc ccgcgccggg cccgttcgcg gagcctcggc gcggggcgcg gcactttcgg
     1261 aggcgtaccg gatgaacttt gaactgagca aggaacatcg aatggcgaga gacctcttcc
     1321 gccgtttcgc ggagaaggaa gcgcggcctc tggcgcagga agtggacgag aacgaacgct
     1381 tcccttcgga gaccgtggag aagatggcgc gctccggctt tttgggtatt ccctgcgcca
     1441 aagagtgggg cgggcagggg tgcgacacgc tgacctacgt cctctgcgtg gaggagctga
     1501 gccgcgtctg cgcgaccacg ggcgtcgtcg tctccgccca cacctcgctg gcctgcgacc
     1561 cgatagagaa gtacggcacg gaggcgcaaa aacggaagta cctcccggag ctcctggcgg
     1621 ggcggaagct gggcgcgttc gcgctcaccg agccgggggc ggggaccgac gcctcgggac
     1681 agcagaccag ggcggtgctt gaaggagaga actaccggct gaacggcagc aagatcttca
     1741 ttacgaacgc cgggcaggcg gatgtgtata tagtctttgc gatgaccgac aaaagcctcg
     1801 gcacgaaggg catctccgcc ttcatcgtgg agaagggctt tcccggattt tcagtcggga
     1861 agaaggaatt gaagatggga atccgcggct cctcgacgtg cgaactcatc ttcgaggact
     1921 gcgtcgtgcc gaaggagaat ctgcttggca aagaggggaa gggcttctcc atcgc
//
LOCUS       DAOTIL010000530         1974 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminobacteriaceae bacterium isolate AMR_MDS_3026
            NODE_68241_length_1974_cov_4.046378, whole genome shotgun sequence.
ACCESSION   DAOTIL010000530 DAOTIL010000000
VERSION     DAOTIL010000530.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868050
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminobacteriaceae bacterium (wastewater metagenome)
  ORGANISM  Aminobacteriaceae bacterium
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae.
REFERENCE   1  (bases 1 to 1974)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1974)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:19:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,224
            CDSs (total)                      :: 3,198
            Genes (coding)                    :: 3,184
            CDSs (with protein)               :: 3,184
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1974
                     /organism="Aminobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_68241_length_1974_cov_4.046378"
                     /isolate="AMR_MDS_3026"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:3034609"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..696)
                     /locus_tag="P5201_10410"
     CDS             complement(<1..696)
                     /locus_tag="P5201_10410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006301062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HRV98995.1"
                     /translation="MSDYSITILNFIAIQAIVACGLNVIVGYAGQISLGHAAFFGIGA
                     YSSALLTTKAGLTFWAALPLVVLITAAIGLVLGMPSLRLREDFLAVTTIGINFIVESV
                     FLYVPFFGGALGLGGIPSIVFFGTRLRGANFLYLCLFFLALVLLISRRFTKSWAGLAC
                     FALREEETAASSMGVSPVRFKLMAFVIGTAMAGLGGALYAHQMRFISATDFGFPLSVM
                     MMSMVVLGGIGTLW"
     gene            complement(710..1573)
                     /locus_tag="P5201_10415"
     CDS             complement(710..1573)
                     /locus_tag="P5201_10415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006301061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0015658 - branched-chain amino acid
                     transmembrane transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HRV98996.1"
                     /translation="MQLFIEQLLNGAAMGAIYALITLGLALVYGVLRILHVAHAGVYA
                     AGAYAGLYVYSHTGSLLLAVLGAMIFCALLGVLIERLIYTPLLKFPPFVPLIGSIAVF
                     LALEEIFRLVGGPYILSFPAELPFSGITVSGVVISPAIISIYAVSASVLLLLWFIVTK
                     TEFGLAMRATSQDREVAGAMGIDGAFTVSMTFALGSAVAAVAGILVGIYFNQVYPTMG
                     AVPAYKCLALIVVGGLGSLPGAVLASLLLGVGETLLIGYANIPLPRDSLAFIAMIAVL
                     LWRPGGLMGNK"
BASE COUNT          420 a          624 c          667 g          263 t
ORIGIN      
        1 ccagagggtg ccgatgccgc cgagcacaac catggacatc atcatgacgg agagcggaaa
       61 gccgaagtcc gtcgcgctga tgaagcgcat ctggtgcgcg tagagcgcgc cgcccagccc
      121 cgccatcgcc gtgccgatca cgaaggccat cagcttgaag cgcaccggcg aaacccccat
      181 gctggaggcc gccgtctcct cctcgcggag ggcgaagcag gccagcccgg cccagctctt
      241 ggtgaagcgc cgcgagatca gcaggacgag cgcgaggaag aagaggcaga ggtagagaaa
      301 gttcgccccc ctgagcctcg tgccgaaaaa gacgatgctc ggaatgccgc cgaggccgag
      361 cgcgccgccg aagaacggga cgtacaggaa gacggactcg acgatgaagt tgatgccgat
      421 ggtggtgacg gcgaggaagt cctcgcgcag acggaggctg ggcatcccga ggacgagccc
      481 gatggccgcg gtgatgagca ccacgagcgg gagcgccgcc cagaaggtga gccccgcctt
      541 ggtggtgagg agcgccgaag agtacgcgcc gatgccgaag aaggcggcgt gccccagcga
      601 gatctgcccg gcgtacccca cgatgacgtt gaggccgcag gcgacgatgg cctgtatcgc
      661 gatgaagttc agaatggtga tgctgtagtc gctcacgtcg ttccctcccc tatttgttgc
      721 ccatgaggcc gcccggcctc cagagcagca cggcgatcat ggcgatgaac gccagggaat
      781 ccctcggcag cgggatgttc gcgtagccga tcagcagcgt ctcgcccacg ccgagcagca
      841 gcgacgcgag caccgccccc ggcagcgagc cgagcccgcc gacgacgatg agggcgaggc
      901 acttgtacgc cggaaccgcc cccattgtgg ggtagacctg gttgaagtag atgccgacga
      961 gaatgcccgc gaccgccgcc acggcggagc cgagggcgaa ggtcatgctg acggtgaacg
     1021 cgccgtcgat ccccatcgcc ccggcgacct ccctgtcctg cgacgtcgcc cgcatggcga
     1081 ggccgaactc ggtctttgtc acgatgaacc agagaagcag caggacggag gcgctcacgg
     1141 cgtagatgga gatgatcgcc ggggaaatca ccacgccgct cacggtgatc ccggaaaagg
     1201 gcagctcggc ggggaaggag aggatgtacg ggccgccgac gaggcggaaa atctcctcca
     1261 gcgcaaggaa aacggcgatg ctcccgatga gcgggacgaa cggggggaac ttcagcagcg
     1321 gcgtgtagat cagccgttcg atgagcacgc cgaggagcgc gcagaagatc atcgcgccga
     1381 ggacggccag aagcagactg ccggtgtgcg agtagacgta caggccggcg taggccccgg
     1441 cggcgtacac ccctgcgtgc gccacatgga gaatgcgcag gacgccgtag acgagggcca
     1501 gaccgagcgt gatcagggcg tagatcgccc ccatcgccgc cccgttcaga agttgctcga
     1561 tgaagagctg catgacggat accaacctcc agaaaactcc ggattgcgtt ccgaaaaaga
     1621 agcttccgcg cggagcgcgc cgggaagttt ctctcagcgg aacgaaaata attctagcac
     1681 acggggggcg gcttcagaaa agccccggta gtgtatacac gcactctccg cgttcgggac
     1741 gtcgaagaaa cagggctttc agccagttcc ggccggcctg ctccgctgcg aaacgcgtcg
     1801 cggaatgtcc tcggtgctcc gcgcgcagct tcacccggcc gaacgggtcg aaccccgtca
     1861 caagcacttt tatcgcgcgc catctccttt cgcaacagtc tcgcccgagg ggaggtcctt
     1921 gccgtatacg acgaaccaga tcgtccctgc ggcgccgaag gccgaggccg tcaa
//
LOCUS       DAOTIL010000531         1974 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminobacteriaceae bacterium isolate AMR_MDS_3026
            NODE_68274_length_1974_cov_2.539865, whole genome shotgun sequence.
ACCESSION   DAOTIL010000531 DAOTIL010000000
VERSION     DAOTIL010000531.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868050
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminobacteriaceae bacterium (wastewater metagenome)
  ORGANISM  Aminobacteriaceae bacterium
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae.
REFERENCE   1  (bases 1 to 1974)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1974)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:19:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,224
            CDSs (total)                      :: 3,198
            Genes (coding)                    :: 3,184
            CDSs (with protein)               :: 3,184
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1974
                     /organism="Aminobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_68274_length_1974_cov_2.539865"
                     /isolate="AMR_MDS_3026"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:3034609"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..883)
                     /gene="hcp"
                     /locus_tag="P5201_10420"
                     /gene_synonym="priS"
     CDS             complement(<1..883)
                     /gene="hcp"
                     /locus_tag="P5201_10420"
                     /gene_synonym="priS"
                     /EC_number="1.7.99.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005660031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016661 - oxidoreductase activity, acting
                     on other nitrogenous compounds as donors [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxylamine reductase"
                     /protein_id="HRV98997.1"
                     /translation="MYCFQCQETAKNQGCTVKGVCGKPEGTANLQDLLIYVCKGIAVY
                     GEKLKQQGTVDREAGRFIIRALFVTITNAAWDDDSIIEWIRKGLKVKDAVAQKAGAAG
                     NLPDCATWKAESKEEILAKAMSAEVRVTAAQNEDVRSLRELVIIGGKGVAAYAEHAEV
                     LGFEKDEIYAFLMEGLASTTKDLSTDEMVGMVMKIGEIAVTTMALLDEANTSTYGNPE
                     ITEVNLGVRNNPGILISGHDLKDMEELLKQTEGTGVDVYTHGEMLPANYYPAFKKYEH
                     FVGNYGGSWWHQNEEFES"
     gene            complement(961..1740)
                     /locus_tag="P5201_10425"
     CDS             complement(961..1740)
                     /locus_tag="P5201_10425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013049280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HRV98998.1"
                     /translation="MNTIMRKIIKIDEEKCDGCGICAEACHEGAIRIIDGKAKLVSET
                     YCDGLGDCIGECPQGAISFEMREAAAYDEEAVKRHMAAKAAGKSSADPLPCGCPGSAA
                     RDLRAAPSATPQARPEAAPAPLQSELVNWPVQIRLVPETAPWLHKAELVIGADCVPFA
                     FPDFHRTFLAGGDTICLIGCPKLDDAQSYVEKLTRIISYNETPAITVVRMEVPCCGGM
                     TRILEAACEASERNISLKIVTIGIRGDILDKETIRFSRRQP"
     gene            complement(1737..>1974)
                     /locus_tag="P5201_10430"
     CDS             complement(1737..>1974)
                     /locus_tag="P5201_10430"
                     /inference="COORDINATES: protein motif:HMM:NF014173.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="Rrf2 family transcriptional regulator"
                     /protein_id="HRV98999.1"
                     /translation="TRGPKGGFELAKRPDEITLFDIYIAIEGVPKGDYCLLHKQQCPF
                     GRCIFGSLLGNITREFVDYLKNTTLGDLQRGEAE"
BASE COUNT          321 a          626 c          578 g          449 t
ORIGIN      
        1 aggactcgaa ctcctcgttc tggtgccacc aggagccgcc gtagttcccc acgaagtgct
       61 cgtacttctt gaacgcgggg tagtagttcg cgggcagcat ttcgccgtgg gtgtacacgt
      121 ccacgccggt accctcggtc tgcttcagaa gctcttccat atccttcagg tcgtggccgg
      181 agatgaggat tcccgggttg ttcctgacgc cgagattgac ttcggtgatc tcggggttgc
      241 cgtaggtgct cgtgttggcc tcgtcgagaa gcgccatcgt ggttacggcg atctcgccga
      301 tcttcatgac catgccgacc atctcgtcgg tggagaggtc cttcgtggtg gaggcaaggc
      361 cttccatgag aaacgcgtag atctcgtcct tctcgaagcc gaggacctcg gcgtgctcgg
      421 cgtaggccgc gacacccttg ccgccgatga tgacgagctc ccgcagcgag cggacgtcct
      481 cgttctgcgc ggcggtgacg cgaacctcgg cggacatggc tttcgcgagg atctcttcct
      541 tgctctcggc cttccacgtt gcgcaatcgg gaaggtttcc ggcggcgccg gccttctgcg
      601 cgaccgcgtc cttgaccttc agccccttgc ggatccactc gatgatggag tcgtcgtccc
      661 acgccgcatt ggtgatggtg acgaaaagcg cgcgaatgat gaaacggccg gcctctctgt
      721 ccaccgtccc ctgctgcttc agcttttcgc cgtatacggc gatgccctta cagacgtaga
      781 tgagaaggtc ctgaagattg gcggtgccct ccggctttcc gcagactccc tttactgtgc
      841 acccctgatt cttcgctgtc tcctgacact gaaaacaata catgccggcg cttcctcctt
      901 tgtgaatttg attttttcga aacatgccga gtacccgttg atacgtctcc gatagtgcgc
      961 tcatggctgt ctcctggaga acctgatggt ctccttgtcc agtatatcac cacgaatacc
     1021 tatcgtcaca attttcagcg atatgttccg ctccgaggcc tcgcatgccg cctccagaat
     1081 gcgcgtcatc ccgccgcagc aggggacttc catacgcacc acggtgatcg cgggcgtttc
     1141 gttgtatgag ataatccgcg tgagcttttc gacgtagctc tgcgcgtcgt cgagcttggg
     1201 gcagccgatg aggcagatcg tatcgccccc ggcgagaaag gttctgtgga agtccgggaa
     1261 ggcgaacgga acgcagtcgg cgccgatcac aagttccgcc ttgtggagcc agggcgcggt
     1321 ctccggcacg aggcgtatct gcaccggcca gttcacaagc tccgactgca gaggcgcggg
     1381 cgccgcttcg gggcgcgcct gcggagtcgc ggacggcgcg gcgcgcagat cgcgggccgc
     1441 cgaaccgggg cagccgcagg gaagaggatc ggcggagctt ttgcccgcgg ccttcgccgc
     1501 catgtggcgc ttcacagcct cttcgtcgta cgccgcggcc tctctcattt cgaagctgat
     1561 ggcgccctgc ggacactcgc cgatgcagtc gcccagcccg tcgcagtacg tttcgctcac
     1621 cagtttcgcc ttgccgtcga tgatccggat tgcgccctcg tggcaggcct cggcgcagat
     1681 gccgcagccg tcgcacttct cctcgtctat cttgatgatc tttctcataa tcgtgttcat
     1741 tccgcttctc ccctctgcaa atctccaagc gttgtgttct tcaggtagtc gacgaactct
     1801 ctcgttatat ttccaagcag cgaaccgaag atgcaccgtc cgaacgggca ctgctgcttg
     1861 tggagcaggc agtagtctcc ctttggaact ccctcgattg cgatgtagat atcgaagagg
     1921 gtgatctcgt ccggccgttt cgccagctcg aaaccgccct tcggcccgcg cgtg
//
LOCUS       DAOTIL010000532         1974 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminobacteriaceae bacterium isolate AMR_MDS_3026
            NODE_68289_length_1974_cov_2.005732, whole genome shotgun sequence.
ACCESSION   DAOTIL010000532 DAOTIL010000000
VERSION     DAOTIL010000532.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868050
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminobacteriaceae bacterium (wastewater metagenome)
  ORGANISM  Aminobacteriaceae bacterium
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae.
REFERENCE   1  (bases 1 to 1974)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1974)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:19:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,224
            CDSs (total)                      :: 3,198
            Genes (coding)                    :: 3,184
            CDSs (with protein)               :: 3,184
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1974
                     /organism="Aminobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_68289_length_1974_cov_2.005732"
                     /isolate="AMR_MDS_3026"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:3034609"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>836
                     /locus_tag="P5201_10435"
     CDS             <1..>836
                     /locus_tag="P5201_10435"
                     /inference="COORDINATES: protein motif:HMM:NF025124.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HRV99000.1"
                     /translation="ISQPIQLRFNELIAGVRGDIAIKLYGDDLTRMGGVAQQVASVLR
                     TVPGAADVKVEQTEGFPTLDVQFLGYASGEPMPETRWIDRMVCEENIVQTWEQLVAKY
                     RFFMPKPFRDIDPVTLGRRDDPPGFRVFREAAVNLLIHQDYGDHSRKAVIKFFRDGIQ
                     FWNPGDVFGDDSRLFEPGEKEVRNPQIAMAMRRISMCEQAGTGMRMMRDEWRKLGHSS
                     PVCRNDRAWKAFELFVPGLDTEADMASDLLKAMFGVVGATEIQPGTDSVPSLSQVCPK
                     SI"
     assembly_gap    837..846
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <847..1057
                     /locus_tag="P5201_10440"
     CDS             <847..1057
                     /locus_tag="P5201_10440"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRV99001.1"
                     /translation="DGTADIVTLMMRAEHTNRTRFRKTILLPLIEASLLEPTIPDKPT
                     SSKQRYRLTTKGRLILEREAPSNEE"
     gene            complement(1129..>1974)
                     /locus_tag="P5201_10445"
     CDS             complement(1129..>1974)
                     /locus_tag="P5201_10445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005658649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P1 family peptidase"
                     /protein_id="HRV99002.1"
                     /translation="GGAPGTRETDLLDPGNMVERIHAVVLAGGSAFGLDAASGVMNVL
                     EERGVGFDVGVTRVPIVCSAVLFDLVCGDWRVRPDKAMGRQAAENAFEPAFIPLRGSV
                     GAGTGALVGKFHGLDFAMRGGLGSFAYRTGPLFVGALVAVNCLGDVMEPGTGNILAGA
                     WDRKKRRFLCTEEAVMNASQEKKNVFAGNTTLGVVATNARLTKAQAKKLASMAHDGFA
                     RSMRPAHTMVDGDTIFALSVGDLEAEMNTLGALAARAVEEAVADAVRSASGGYGFPSV
                     SDIRS"
BASE COUNT          377 a          603 c          605 g          379 t
ORIGIN      
        1 gatcagtcag ccgatccagc tgcgtttcaa cgagctgatc gccggggtgc gcggcgacat
       61 cgcgatcaag ctctacggtg acgatctcac ccggatgggc ggggtggcgc agcaggtcgc
      121 gagtgtactg cgcaccgtgc cgggcgcggc cgacgtcaag gtcgagcaga ccgagggctt
      181 cccgacgctc gacgtgcagt ttctcgggta tgcaagcggc gaaccgatgc ctgaaacgcg
      241 ctggatagac aggatggtct gtgaggagaa tattgttcag acgtgggagc agctcgttgc
      301 caagtacagg tttttcatgc ccaaaccgtt ccgcgatatc gacccggtga ctctggggcg
      361 acgcgacgat cctccgggct tccgcgtttt tcgtgaagcc gcagtaaacc ttctgatcca
      421 tcaggattat ggagatcact cccgcaaggc ggtcatcaaa tttttccggg acggtattca
      481 gttctggaat ccgggagacg tcttcggcga cgattcgcgc cttttcgagc ccggagaaaa
      541 ggaggttcgc aatccgcaga tcgcgatggc gatgcggcgt atctccatgt gcgaacaggc
      601 cggtacgggg atgcggatga tgcgggacga gtggcgaaag ctggggcact cgtcgcctgt
      661 ctgcagaaat gatcgcgcgt ggaaagcgtt cgagctgttc gttccgggat tggacacaga
      721 agcggatatg gcatcggatt tgttgaaagc gatgttcggt gttgtcggtg cgaccgaaat
      781 tcaacctggg acagattctg taccaagtct gtcccaggtg tgtcccaagt ctattgnnnn
      841 nnnnnncgac ggtacagccg acattgtaac gttgatgatg agagctgaac acaccaatcg
      901 aacgcgtttt cgaaagacga ttctacttcc gctcatcgaa gcgtcgctct tggagccaac
      961 cattcccgac aaaccgacca gcagcaaaca gcggtatcgc ctgacgacga aaggacgtct
     1021 gatcctggaa agggaagcgc cttccaacga ggaataaaac agcccgcccc cggcacaggc
     1081 tgttccgggg gcgggctgtt ctttcaggtt gccgcttcgt tgtttttttc agctccggat
     1141 atccgatacc gacgggaagc cgtaccctcc actcgccgag cgcaccgcgt ccgcgacagc
     1201 ctcctccacc gcgcgggcgg cgagcgcgcc gagggtgttc atctcggcct ccagatcccc
     1261 gactgagagg gcgaagatgg tgtcgccgtc caccatcgtg tgcgccggac gcatcgagcg
     1321 ggcgaagccg tcgtgcgcca tggaggcgag cttcttcgcc tgcgccttgg tgagccgcgc
     1381 atttgtggcg acgacgccga gcgtcgtatt cccggcgaag acgttctttt tctcctgaga
     1441 agcgttcatc accgcctcct cggtgcacag gaagcggcgc ttctttctgt cccacgctcc
     1501 ggcgaggatg ttgcctgttc cgggttccat cacatccccg agacagttga ccgcgacgag
     1561 cgcgccgacg aacaaaggcc ccgtcctgta ggcgaaggac ccgagtcctc cgcgcatcgc
     1621 gaagtcgagt ccgtggaact tgccgacgag cgctccggtt cccgcgccca ccgatcctcg
     1681 aagaggaatg aacgcaggtt cgaaggcgtt ttcggcggct tgccgcccca tcgctttgtc
     1741 aggacgcacc cgccagtcgc cgcagaccag atcgaagagc actgcggagc agacgatggg
     1801 gaccctggtt acgccgacgt cgaagcctac tcccctctcc tccagaacgt tcatcacacc
     1861 cgaggccgcg tccagtccga aggcgctgcc ccccgcgagg accaccgcgt ggattcgctc
     1921 caccatattg cccgggtcca gcaggtcggt ctcgcgcgtt cccggagcgc cgcc
//
LOCUS       DAOTIL010000533         1970 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminobacteriaceae bacterium isolate AMR_MDS_3026
            NODE_68450_length_1970_cov_8.390601, whole genome shotgun sequence.
ACCESSION   DAOTIL010000533 DAOTIL010000000
VERSION     DAOTIL010000533.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868050
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminobacteriaceae bacterium (wastewater metagenome)
  ORGANISM  Aminobacteriaceae bacterium
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae.
REFERENCE   1  (bases 1 to 1970)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1970)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:19:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,224
            CDSs (total)                      :: 3,198
            Genes (coding)                    :: 3,184
            CDSs (with protein)               :: 3,184
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1970
                     /organism="Aminobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_68450_length_1970_cov_8.390601"
                     /isolate="AMR_MDS_3026"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:3034609"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..278
                     /locus_tag="P5201_10450"
     CDS             <1..278
                     /locus_tag="P5201_10450"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRV99003.1"
                     /translation="QSAGFATSTFREEFSSSRRAERLTKNAATVETSGKRTVRRMRNC
                     CEDIACGGKEKRSGRYEKNAINTKNPSGPVAKTGVKSVHHKQTPPER"
     gene            286..1755
                     /locus_tag="P5201_10455"
     CDS             286..1755
                     /locus_tag="P5201_10455"
                     /inference="COORDINATES: protein motif:HMM:NF025080.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HRV99004.1"
                     /translation="MPRKTAPFNFESAPEQRSLTAKPGGILLAELFHASGCAAAADAI
                     GVRSSRGYSDADHVLSSVLLNALGFDAGDDLEQLRSDGALVSAVHLLNEHKGKERRTV
                     NAFPSPSAFRAFLTDAGSTEERVRGFSGLCSVPVRCAQEMKPQEKITVDMDATIIPTE
                     RGDDVRCNYKGERSHMAFNSYVPELDMVVHSEFCPGNVSPRDDQLGLLRRTLSKLPEG
                     VKEVETRIDSAGYCMDLIKYCCGGESPYGVIRFSISVPMYKTYREEIERLSEKAWSPF
                     VDENGREETDRRWAEVNIVPTSLVSSEPYPEIRFIAVRERMKHPKLKERMDAAQMELA
                     IEEIEGEHPKTERLHLTCMDGTVWKVFLMVTNDFERNGQHALEHHWKRCGKSEEVHGI
                     LKNDLAGGHVPSADFGSCAVWWYLCVLSYNLQRLMTHRLFPEEWARAHQKRLLATIYS
                     TPAKMVRHGKQIVIRVFGLLGRLIEAAHWKVLRLGRLNI"
BASE COUNT          461 a          534 c          625 g          350 t
ORIGIN      
        1 ctcaatcggc gggattcgca acttccacct ttcgggagga gttttcgagt tcgaggcggg
       61 cagaacggct tacgaaaaat gctgcgaccg tggaaacatc aggaaagcga actgttcgcc
      121 ggatgaggaa ttgctgcgaa gatatcgctt gcggcggaaa agaaaagagg agcggaagat
      181 acgagaaaaa cgcaataaat acaaaaaacc cctctggtcc cgttgcgaaa acgggtgtaa
      241 agtcggttca ccacaaacaa actccaccag agaggtgacc gtattatgcc acgaaagacc
      301 gctccgttca acttcgaatc cgcccccgaa cagagaagcc ttaccgcgaa gccgggagga
      361 atccttctgg cggaactctt ccacgcatcg ggctgcgcgg cggccgcgga cgcaataggg
      421 gttcggagca gcaggggcta ctccgacgcc gaccatgtgc tttcgtcggt gctgctgaac
      481 gctctcggct tcgacgcggg cgacgatctg gaacagctcc gctccgacgg ggcgctcgtc
      541 tccgccgtac atcttctgaa cgagcacaag gggaaggaga gacgcaccgt gaacgccttt
      601 ccctcccctt cggccttccg cgcctttctg accgacgcgg gaagcacgga ggagagagtg
      661 cgcggattct ccggtctgtg ctccgtcccc gtccgttgcg cgcaggaaat gaaaccgcag
      721 gagaagatca ccgtcgacat ggacgccacc atcatcccta cggagcgcgg cgacgatgtc
      781 cggtgcaact acaagggcga gagatcgcac atggcgttca acagctacgt tcccgagctg
      841 gacatggtgg ttcacagcga attttgtccc ggaaacgtat cgccccgcga cgaccagctc
      901 ggactgcttc gaagaacgct ctcgaagctg cccgaggggg tgaaggaggt cgaaacgcgc
      961 atcgacagcg ccggatactg catggatctc atcaagtact gctgcggcgg agagagcccg
     1021 tacggcgtca tacgcttctc catctccgtt cccatgtaca aaacgtaccg ggaggagatc
     1081 gaaagacttt ccgagaaggc ctggagcccc ttcgtggacg aaaacggcag ggaggagacc
     1141 gaccggcggt gggccgaagt gaacatcgtg ccgacctcgc tcgtttcgag cgagccctat
     1201 ccggaaatcc gcttcatcgc cgtgcgggag aggatgaagc atccgaagct gaaggagaga
     1261 atggacgccg cgcagatgga gctcgcgata gaagagatcg agggggagca tccgaagacg
     1321 gagcgactgc acctgacctg catggacgga acggtctgga aggttttcct gatggtgacc
     1381 aacgacttcg agcggaacgg acagcacgcg ctcgagcatc actggaagcg atgcgggaag
     1441 tccgaggagg tgcacggaat cctgaagaac gacctcgccg ggggccacgt gccgagcgcc
     1501 gacttcggct cctgcgcggt ctggtggtat ctgtgcgtcc tgtcgtacaa ccttcagcgg
     1561 cttatgacgc atcgtctctt tccggaggag tgggcgcgcg cgcatcagaa gcggttgttg
     1621 gcgacgatct actcgacgcc tgcgaagatg gtgcgccacg gaaagcagat agtgatacga
     1681 gtgttcggtc ttcttggaag actgatcgaa gccgcgcatt ggaaggtgct gcggctcggt
     1741 cgcctgaata tttgatattc acggagggag gggtgatgcg atagcgaaga cgccgccgcc
     1801 gagtggagcg cggcgcggat ggagtgtact ttcattgcga caaactcagc ctttctgtgg
     1861 ataaaaatga atgcgggagg gtgtttttcg acatccgggt aatgttcgga taaaaggggt
     1921 tgaaaaaaac ctttgcggaa gcctcgtcaa aaacaacctg tggaacgagg
//
LOCUS       DAOTIL010000534         1967 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminobacteriaceae bacterium isolate AMR_MDS_3026
            NODE_68600_length_1967_cov_2.862971, whole genome shotgun sequence.
ACCESSION   DAOTIL010000534 DAOTIL010000000
VERSION     DAOTIL010000534.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868050
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminobacteriaceae bacterium (wastewater metagenome)
  ORGANISM  Aminobacteriaceae bacterium
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae.
REFERENCE   1  (bases 1 to 1967)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1967)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:19:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,224
            CDSs (total)                      :: 3,198
            Genes (coding)                    :: 3,184
            CDSs (with protein)               :: 3,184
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1967
                     /organism="Aminobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_68600_length_1967_cov_2.862971"
                     /isolate="AMR_MDS_3026"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:3034609"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            166..459
                     /locus_tag="P5201_10460"
     CDS             166..459
                     /locus_tag="P5201_10460"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRV99005.1"
                     /translation="MDDPSFEYAKRPEGRVPSGLYSLRNSDDSIPRVRNCYGCGVSPA
                     GTGRPALPDQPEEEEELLPMPLKRTVRAIKIRSLKKDVHVISAKAINVNRFLR"
     gene            609..1598
                     /locus_tag="P5201_10465"
     CDS             609..1598
                     /locus_tag="P5201_10465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008710512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_component: GO:0031317 - tripartite ATP-independent
                     periplasmic transporter complex [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_function: GO:0005488 - binding [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DctP family TRAP transporter solute-binding
                     subunit"
                     /protein_id="HRV99006.1"
                     /translation="MYGIAALAAVLTVVLTGAAFAAPEYVIKVGYILPENQSDHIIMR
                     DVFKKDIEEKSGGRISVELYPNAQLGGDRELIESVQLGTVQVAIPATSALAGFDKRFQ
                     VFDLPFLFKSKETAFKALDGELGHKVDELLKPLGMRNLGYGENGYRHITNSRGPVHKP
                     EDLKGIKLRTMENPLHLAFFKMLGANPTPMSFGELYTALQQGTVDGQENPVVLVYTSK
                     FYEVQKFYSLTGHVYSATMLVANDDFFASMPEDLWKIVEDAGKRYVVEQRALAEVQEQ
                     EFLEELKKTGLQINELTPEEKQLFIDATLPAYDQFKDVIGAELVELAKKANEE"
     gene            1693..>1967
                     /locus_tag="P5201_10470"
     CDS             1693..>1967
                     /locus_tag="P5201_10470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006299796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter small permease"
                     /protein_id="HRV99007.1"
                     /translation="MRKAFDRVEEVFLVGSLAFNVVLIFIQVVMRYVFQNSLSWSEEL
                     ARYIFLWQTWVGASYAVRMRRHFRVEMLVDLMKGRLRKWYELVVLLV"
BASE COUNT          450 a          546 c          598 g          373 t
ORIGIN      
        1 gtaatcacgg gctgcgaaac gcgcaacagc ttcttcagca gcgcgacgat ctcctccagc
       61 agcgcgaagt acgcttcgct tctgtgcccg acgagaggcg cggcgcccgc agcgcgaacg
      121 gaatgcggaa cctcgaccgg tcccggcgtg aaaagatggg ggtgcatgga cgatccctcc
      181 tttgaatatg cgaaaaggcc ggaagggcgt gtcccctccg gcctctattc cctgcgaaat
      241 tccgatgatt caattccaag ggtgcgaaac tgctacggct gcggcgtctc ccctgccggc
      301 acaggaagac cagcccttcc ggaccaaccg gaagaggagg aggaactact acccatgccg
      361 ttgaaaagga ctgtacgcgc cataaaaatc cgctccctga aaaaagatgt tcacgttata
      421 tcagccaaag ctatcaatgt caataggttt ttacgataat gcgataatac gtaccagagg
      481 cttcgttatg aaaaaagact ataccttact actgcgctgt agtattatag caatcgcaga
      541 aaatcttccg gatacggaag gccggagtat gagccggtat atcaaaagga ggggttgtaa
      601 tgaagcgtat gtacggaata gcggcattgg cggcggttct tacggtggtg ttgacgggcg
      661 cggcgttcgc ggcgccggag tatgtgatca aggtcgggta tattcttccg gagaaccagt
      721 cggaccacat catcatgcgg gatgtgttca agaaggatat cgaggagaag agcggcggca
      781 ggatctccgt cgaactctac cccaacgcgc agctcggcgg cgacagggag ctcatcgaat
      841 ccgtccagct ggggacggtg caggtggcca tcccggcgac gtcggcgctc gccggtttcg
      901 acaagcgctt ccaggtgttc gacctcccct tcctcttcaa gtcgaaggaa acggcgttca
      961 aggcgctcga cggcgaactc ggccataagg tcgacgagct gctgaagccc cttggaatgc
     1021 gcaacctggg gtacggcgag aacggctaca ggcacatcac caacagccgc ggccccgttc
     1081 acaagccgga agacctgaaa ggaatcaagc tgcgcaccat ggagaacccg ctccacctcg
     1141 ccttcttcaa gatgctcggc gcgaacccca cgcccatgag cttcggcgaa ctctacaccg
     1201 cgctccagca gggcacggtg gacggacagg agaaccccgt cgttttggtg tacacctcca
     1261 agttctacga ggtgcagaag ttctattcgc tcacgggaca cgtttactcc gcaacgatgc
     1321 tcgtggctaa cgacgacttc ttcgcgtcga tgcccgagga tctctggaag atcgtcgagg
     1381 acgcgggcaa gcgctacgtc gtcgagcagc gggcgctcgc ggaggtgcag gagcaggagt
     1441 tcctcgaaga gctcaaaaag acgggactcc agatcaacga gctgacgccc gaagagaagc
     1501 agctcttcat cgacgccacg cttcccgcct acgaccagtt caaggacgtc atcggcgcgg
     1561 agctcgtcga gctggcgaag aaagccaacg aagagtaggg gagcatccgt tcacagaaca
     1621 ggagaatcgg tgttttcgcg ccgcaaggga gcagcagccc cttgcggtgc gctgtaaaga
     1681 ggagggagaa gagtgcgaaa agccttcgac agagtcgagg aagtctttct tgtaggcagt
     1741 cttgccttca acgtggtcct tatctttatc caggtggtca tgcggtacgt gttccagaac
     1801 tcgctttcgt ggagcgagga gcttgcccgc tacatctttc tctggcagac gtgggtgggg
     1861 gcgagctacg cggtgcggat gcggcgtcac ttccgcgtgg agatgctcgt cgacctcatg
     1921 aaggggcgcc tccgcaagtg gtatgaactt gtcgtgctgc tggtgtg
//
LOCUS       DAOTIL010000535         1966 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminobacteriaceae bacterium isolate AMR_MDS_3026
            NODE_68638_length_1966_cov_3.457875, whole genome shotgun sequence.
ACCESSION   DAOTIL010000535 DAOTIL010000000
VERSION     DAOTIL010000535.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868050
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminobacteriaceae bacterium (wastewater metagenome)
  ORGANISM  Aminobacteriaceae bacterium
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae.
REFERENCE   1  (bases 1 to 1966)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1966)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:19:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,224
            CDSs (total)                      :: 3,198
            Genes (coding)                    :: 3,184
            CDSs (with protein)               :: 3,184
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1966
                     /organism="Aminobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_68638_length_1966_cov_3.457875"
                     /isolate="AMR_MDS_3026"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:3034609"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..569)
                     /locus_tag="P5201_10475"
     CDS             complement(<1..569)
                     /locus_tag="P5201_10475"
                     /inference="COORDINATES: protein motif:HMM:NF016629.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rpn family recombination-promoting
                     nuclease/putative transposase"
                     /protein_id="HRV99008.1"
                     /translation="MGDKDRAFRRLLADERLFLKFLRRFLKQNLPDVIDVEALTLDDL
                     HREDITFIPPDLSERESDVLYRIRGTDAEAYVYVLVEHQSSVSHLMPFRLLSYVVQFW
                     TRYVEKVGEASKRKDFLLPPVLPVVFYEGATRWTAPVHFAEKVRNAYDFGKFIPAFEY
                     RLISLRDKNPESLLSYGDALGALFYLANPS"
     gene            854..1297
                     /locus_tag="P5201_10480"
     CDS             854..1297
                     /locus_tag="P5201_10480"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRV99009.1"
                     /translation="MDISRKRSALMVLIALLWLSITALGMLKLFLPAMILGVVLMFVH
                     MFLGVARKGVVSKAFLVYPLLAWGMLWGASFILSKHYSDAFAGQMPAFTILGLHPSFA
                     WTVLTYWIGGMLTLTVGFMFLRSEWLSDKDWDEFLATIASGKEGA"
     gene            1300..>1966
                     /locus_tag="P5201_10485"
     CDS             1300..>1966
                     /locus_tag="P5201_10485"
                     /inference="COORDINATES: protein motif:HMM:NF012686.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRV99010.1"
                     /translation="MEKTLIIGFSVAMIVASFLIGWWAKRKVTSNKAFFGSTGMFGPL
                     AVGLSSTAAVASAFALVGVPGIIYATGNPMTFWMLSSAAFTMAYIILGKKVRAMAEIG
                     EVASLGDISDLRFNNNRLIKALLSVVIFLGCIAYLSAQIKAGSELFAHLLGWEPIVAA
                     FVIFGILTVYTAISGEVGGLLTQAFQGLVMVVAGVIMIVSFYAMTGGFGNVMQAVGSL
                     KEAV"
BASE COUNT          374 a          570 c          580 g          442 t
ORIGIN      
        1 gagggattcg ccaggtagaa gagcgcgccc agggcgtctc cgtaggagag aagcgattcg
       61 gggttcttgt cgcgcagcga gatcagccga tactcaaagg ccggaatgaa cttgccgaag
      121 tcgtaggcgt tccgaacctt ctccgcgaag tgcaccggcg cagtccacct ggttgcccct
      181 tcgtagaaaa ctaccggcag caccggaggc agtagaaaat ccttgcgctt cgaggcttca
      241 ccgaccttct cgacataccg tgtccaaaac tgaacaacgt acgaaagaag acggaatggc
      301 atcaggtggc tcacgctcga ctgatgctcc accagcacgt agacgtaggc ctcggcgtcg
      361 gtcccccgga tgcggtataa aacgtcggat tcccgctcgg agaggtccgg agggatgaac
      421 gtgatgtcct cgcgatgcag gtcgtcgagc gtgagcgctt ccacatcgat aacgtccggc
      481 aggttctgct tcaaaaacct tcggagaaac ttcagaaaga gccgctcgtc ggccagaagc
      541 ctgcgaaacg ccctgtcctt gtcgcccatg ggactcctct tttccacaaa aagctttttg
      601 attattatag cacgcttgat ggagctgccg ctttgcgcgt gtttccggag ttgttcatgt
      661 cgtgcaccgg cggaatttcc ggttccatag actgaaataa cagaaaaatg acggaaggtc
      721 cttgacaggc ttcggggagg agtgctattc tgtgcgcatt atgaagcatg attcatgttt
      781 catttcgacg cgcccggtaa attgccggac cgggaccgtc gaggcgtcgc acattcgaag
      841 gaggggatag gtaatggata tttccaggaa gagatccgca ctcatggtcc tcatcgcgtt
      901 attatggctt agcatcactg cgttgggcat gttgaagctg tttttgcccg caatgatcct
      961 cggagttgtt cttatgttcg tgcacatgtt tctcggcgtg gcccgcaaag gcgtcgtctc
     1021 gaaggcgttt ctggtctacc cgctgctggc gtggggcatg ctctgggggg cgagcttcat
     1081 cctctccaag cactattccg acgcgttcgc ggggcagatg ccggccttca ccattctcgg
     1141 ccttcacccc tctttcgcgt ggaccgtgct cacctactgg atcggtggta tgctgacgct
     1201 gacggtcggg ttcatgttcc tcaggagcga atggctgtcc gataaggact gggacgagtt
     1261 tctggcgaca atcgcaagcg gcaaggaggg tgcgtaacta tggaaaagac actcatcatc
     1321 gggttttccg tggcgatgat cgtcgcctcc ttcctcatcg gatggtgggc gaagagaaag
     1381 gtaacctcca ataaggcctt cttcggcagc accggcatgt tcggtccgct cgcggtcggc
     1441 ctctccagca ccgccgcggt ggccagcgcg ttcgctctgg tgggcgtccc cggcatcatc
     1501 tacgcgaccg gcaaccccat gaccttctgg atgctctcct ccgcggcctt caccatggcc
     1561 tacatcatcc tcggcaagaa ggtccgcgcg atggccgaga tcggcgaagt ggccagcctg
     1621 ggcgacatct ccgacctccg tttcaacaac aacaggctca tcaaggccct tctctccgtc
     1681 gtcatcttcc tcggctgcat cgcctacctc tccgcgcaga tcaaggccgg ttcggagctc
     1741 ttcgcccatc ttctggggtg ggagccgatt gtcgccgcgt tcgtcatctt cggtatcctc
     1801 acggtctaca ccgcgatcag cggcgaggtc ggcggtctgc tgacgcaggc gttccaggga
     1861 ctggtgatgg tggtggcggg cgtgatcatg atcgtctcct tctacgccat gaccggcggc
     1921 ttcggcaacg tcatgcaggc agtgggttcg ctcaaggagg ctgtcg
//
LOCUS       DAOTIL010000536         1963 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminobacteriaceae bacterium isolate AMR_MDS_3026
            NODE_68780_length_1963_cov_4.662998, whole genome shotgun sequence.
ACCESSION   DAOTIL010000536 DAOTIL010000000
VERSION     DAOTIL010000536.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868050
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminobacteriaceae bacterium (wastewater metagenome)
  ORGANISM  Aminobacteriaceae bacterium
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae.
REFERENCE   1  (bases 1 to 1963)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1963)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:19:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,224
            CDSs (total)                      :: 3,198
            Genes (coding)                    :: 3,184
            CDSs (with protein)               :: 3,184
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1963
                     /organism="Aminobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_68780_length_1963_cov_4.662998"
                     /isolate="AMR_MDS_3026"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:3034609"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            379..786
                     /locus_tag="P5201_10490"
     CDS             379..786
                     /locus_tag="P5201_10490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013047680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin/lipoyl-binding protein"
                     /protein_id="HRV99011.1"
                     /translation="MSKKYRITVDGTAYTVEVEDLGAGAPAASAPAAAAAPAPAPVAA
                     PAPAAAPAPAAAAPAPAAGGAGVPVTAPMPGKVLKVTVSAGAAVKNGDLVLVLEAMKM
                     ENEIFSPADGVVKEIRSREGDAVNSGDVMMVVG"
     gene            843..>1963
                     /locus_tag="P5201_10495"
     CDS             843..>1963
                     /locus_tag="P5201_10495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013047681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium ion-translocating decarboxylase subunit
                     beta"
                     /protein_id="HRV99012.1"
                     /translation="MEIYMQAIREIIVSSGFAGLTGGHLMMIVVALVMLYLAIGKGFE
                     PLLLVPIGFGCLLVNLPLSGIMDDGGFLRYVFFGTEHEIYPVIIFMGIGALTDFAPLL
                     ANPITFLLGAAAQFGVFIALFGAMLMGFSVNEAASIAIIGGADGPTSIYLTMKLAPQI
                     LGAVAVAAYSYMSLVPLIQPPVIKMLTTKADRKIRMDTLRPVTKLEKILFPIVTTMAA
                     GLILPASVPLVGILMFGNLVRECGVTERLSQAAQNEILNTTTIFLGLSVGATMNAEEF
                     LTAATLKIILLGLVAFIFSTAGGVLFGQLMKKWSGGKINPMIGAAGVSAVPMAARVVQ
                     RVAQKESPGNFLLMHAMGPNVAGVIGTAVAAGVMLTLLS"
BASE COUNT          444 a          598 c          623 g          298 t
ORIGIN      
        1 ccgacgggcg tctcatggca gtgctcgccg ccgcagtagc cgccgcagga atcggcggcc
       61 gcatcaccgg agtgacggta gccggaggca gccgcgcccg ccccgcccga gcggccggat
      121 ggagaagcgc ctcgctcgcc gaaggaatcc agagcctctc ccgggactgg aaatagcgcc
      181 gcagccgcgc gcacgcccgg agaagcgaag aagcaataaa gaagcaataa agcagcaata
      241 aagcagcaat aaagaagtat tagaagtatt aaaagtatta aatagcaagc gaaaagcaaa
      301 acagccaaga gacgaataaa gaacggcaac acacagagaa cactcgaaac caaacgaaag
      361 aaggaggctc atcgcacaat gagcaaaaaa tacagaatca cagtcgacgg aacagcctac
      421 acagtagaag tggaagacct cggagcgggc gcccctgcgg cgtcggcccc ggcagctgcg
      481 gcagccccgg ctccggcccc ggtagcggcg cccgcaccgg cagccgcccc cgcccctgcg
      541 gcggcagccc ccgcgcccgc agccggaggc gccggagtcc ccgtcaccgc ccccatgccc
      601 ggcaaagtcc tcaaagtaac cgtaagcgcc ggagccgcgg tcaagaacgg agacctcgtc
      661 ctcgtcctcg aagccatgaa gatggagaac gaaatcttct cccccgccga cggagtcgtc
      721 aaggagatcc ggagccgcga aggggacgcc gtcaactccg gcgacgtcat gatggtcgta
      781 gggtagcccc gggaccagga cacaacctgt agagacagga gcaaggaggg agaccgaggg
      841 caatggaaat ctacatgcaa gcgataagag agatcatcgt atcgtccgga ttcgccggac
      901 tcaccggagg acacctcatg atgatcgtcg tggcgctggt gatgctctac ctcgccatag
      961 ggaaaggatt cgaaccgctg ctgctcgtac cgatcggatt cggctgtctg ctggtgaacc
     1021 tgccgctgtc ggggataatg gacgacggag ggttcctgcg gtacgtcttc ttcggaacgg
     1081 aacacgaaat ctaccccgtc atcatcttca tgggaatagg cgccttgacc gacttcgcgc
     1141 cgctgttggc caaccccatc acgtttttgc tcggagcggc ggcgcagttc ggagtcttca
     1201 tcgcgctctt cggcgccatg ctgatgggat tctccgtcaa cgaagccgcg tcgatcgcca
     1261 tcataggagg agcggacggc cccacgagca tctacctcac catgaagctc gcgccgcaga
     1321 tccttggcgc cgtggctgtg gcggcgtaca gctacatgtc gctcgtgccg ctcatccagc
     1381 cgccggtcat caagatgctc acgaccaagg ccgaccggaa gattcgaatg gacacgctgc
     1441 ggcccgtcac gaagcttgag aagatcctct ttccgatagt gacgacgatg gcggcgggcc
     1501 tgatcctccc cgcgtcggtg ccgctggtgg gcatccttat gttcgggaac ctcgtgcgtg
     1561 aatgcggggt cacggaacgg ctgagccagg cggcgcagaa cgagatcctg aacaccacga
     1621 cgatcttcct gggactgtcg gtgggagcga ccatgaacgc cgaggagttt ctgacagcgg
     1681 cgacgctgaa gatcatattg ctgggacttg tagcgttcat attcagcacg gcgggaggcg
     1741 tcctgttcgg ccagctgatg aagaagtgga gcggcgggaa gatcaacccg atgatagggg
     1801 cggcgggagt ctcggcggtg ccgatggcgg cgcgcgtcgt gcagcgagtg gcgcagaagg
     1861 agagccccgg gaacttcctg ctcatgcacg ccatgggccc gaacgtcgcc ggagtcatag
     1921 gcacggccgt cgccgcaggc gtcatgctta cgctgctgag ctg
//
LOCUS       DAOTIL010000537         1962 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminobacteriaceae bacterium isolate AMR_MDS_3026
            NODE_68870_length_1962_cov_2.530152, whole genome shotgun sequence.
ACCESSION   DAOTIL010000537 DAOTIL010000000
VERSION     DAOTIL010000537.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868050
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminobacteriaceae bacterium (wastewater metagenome)
  ORGANISM  Aminobacteriaceae bacterium
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae.
REFERENCE   1  (bases 1 to 1962)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1962)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:19:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,224
            CDSs (total)                      :: 3,198
            Genes (coding)                    :: 3,184
            CDSs (with protein)               :: 3,184
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1962
                     /organism="Aminobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_68870_length_1962_cov_2.530152"
                     /isolate="AMR_MDS_3026"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:3034609"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..896
                     /locus_tag="P5201_10500"
     CDS             <1..896
                     /locus_tag="P5201_10500"
                     /inference="COORDINATES: protein motif:HMM:NF020534.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0098609 - cell-cell adhesion [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="beta/gamma crystallin domain-containing protein"
                     /protein_id="HRV99013.1"
                     /translation="SLKVGKGTRITLYQQANYGGASISFEGDGTYANDVSDLNGLGWG
                     DMISSFKVRQCAIPEANQAFLFERENYDGAGLYLTVGQDIPDLRQWAISSGKSWNDRI
                     SSLKIGKDALVHVWTDSNYEGPSKPYSGDGQGYVAITSLHPEGWGDKISSLKVRTPGW
                     TPESELPGEPEEPEEPEGEQPADWKDIEPEPHQVIIYEDIDFEGAAIGYEYDSDVLDL
                     TKLLVLDTNKNWNDRISSVKIGKNAKVVLYENIGCDDRTAFITLQGNGQSVSRYDSLH
                     SIGWGDKVSSFRVRMSDYYGR"
     gene            complement(862..1404)
                     /locus_tag="P5201_10505"
     CDS             complement(862..1404)
                     /locus_tag="P5201_10505"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRV99014.1"
                     /translation="MRKYSGAARLTALALSFVLVTQPLAFAAPPASPAAANEEERAFD
                     RLVQDFESTCALLEDALPEAVFPRAESDEELAALYKRLVDNLRAALAYSVGIRFSGGT
                     GRLNVLPREERAAAVMQKMPPRMELILDASGSMNRRIGGKDSLRTMCSANRESRSRTA
                     SFPACREERIIFRSSRSSGL"
     gene            complement(1513..>1962)
                     /locus_tag="P5201_10510"
     CDS             complement(1513..>1962)
                     /locus_tag="P5201_10510"
                     /inference="COORDINATES: protein motif:HMM:NF014069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing phosphohydrolase"
                     /protein_id="HRV99015.1"
                     /translation="RALGLPEPETRRLREMGLLHDIGKIAVDEALLNKNGTFTNEERK
                     NIEQHPAVGYRILNLFDDTIDLAEGVLYHHENWDGSGYPIGARGEEIPLHARILRVAE
                     TYDALRGAFHSSAMSSEEALAEIERHSGGMFDPEIVRIFVGMMSGAK"
BASE COUNT          417 a          602 c          594 g          349 t
ORIGIN      
        1 gctcgctcaa agtaggcaag ggaacgcgca tcacgctcta tcagcaggcc aattacggag
       61 gcgcaagtat cagtttcgag ggggacggaa cgtacgccaa cgatgtttcc gatctgaacg
      121 ggttggggtg gggagacatg atctcctcct tcaaggtacg ccagtgcgcc atccccgaag
      181 cgaaccaggc gttccttttc gagcgcgaga actacgacgg cgccggcctc tacctcaccg
      241 tcgggcagga catccccgat ctcaggcagt gggcgatatc gagcggaaag agctggaacg
      301 acagaatcag ctcgctgaag atcggcaagg acgcgctggt ccatgtgtgg acggacagca
      361 actacgaggg cccctcgaaa ccttattcgg gagacgggca gggctacgtg gccataacct
      421 ccctgcaccc cgaagggtgg ggagacaaaa tctcctcgct gaaagtgcgc acccccggct
      481 ggacccccga gagcgaactg cccggggaac cggaggagcc ggaagagccg gagggagaac
      541 agcccgccga ctggaaagat atcgagccgg aacctcacca ggtcatcatt tacgaggata
      601 tcgatttcga aggggcggcc atagggtacg agtacgacag cgacgtgctg gatctgacga
      661 aactgctcgt cctggacacc aataagaact ggaacgacag gatcagctcc gtcaagatag
      721 gcaaaaacgc gaaggtcgtc ctctacgaga acatcggatg cgacgacagg accgccttca
      781 tcacgcttca gggaaacgga cagagcgtct ccagatacga ctcgctgcac tcgatcggct
      841 ggggcgacaa ggtgtcctcg ttcagagtcc ggatgagcga ctactacgga agataattct
      901 ctcttcgcgg catgccggaa acgacgccgt gcgcgaccgg ctctcccgat tcgccgagca
      961 catggtacgg agggaatcct tccctccgat acgccggttc atcgagccgg acgcgtcgag
     1021 gatcagctcc atcctcggcg gcatcttctg cataaccgcc gctgcgcgct cctcgcgggg
     1081 gagcacgttg agccgccccg tcccgccgct gaagcggatg ccgacggagt aggcgagggc
     1141 ggcgcggaga ttgtccacga gccgcttgta gagcgcggca agctcctcgt cggactccgc
     1201 gcgcggaaaa acggcctccg ggagcgcgtc ttcaagcagc gcgcacgtgc tctcgaagtc
     1261 ctgcacaaga cgatcgaacg ccctctcctc ctcgttcgcc gccgccggag aagcgggggg
     1321 tgcggcaaac gcaagaggct gcgtcaccag aacaaacgac agcgcgagag ccgtcagacg
     1381 ggctgctcca gagtattttc gcatgctgct ccctccccgg attcctgagt agttttacaa
     1441 gacagtacac atccggcggg aaaaagtcaa tagaattggg atttttcgtt atatatgcgc
     1501 cgggagcgcc ccttacttcg ccccggacat catgccgaca aagatcctca cgatctccgg
     1561 gtcgaacatg ccgcccgaat gccgctctat ctccgccagc gcttcctcgg aactcatcgc
     1621 gctgctgtgg aaggcgcctc taagcgcgtc gtaagtttcc gcgacgcgga gtattctcgc
     1681 atgcagtgga atctcttctc ccctcgcgcc gatagggtac cctgacccgt cccagttctc
     1741 gtggtgatag aggacgccct cggccagatc gatcgtatcg tcgaacaggt tcagaattct
     1801 gtagccgacg gcggggtgct gctcgatgtt tttccgctct tcgttggtga acgttccgtt
     1861 tttgttcaac agggcctcgt cgacggcgat cttgccgatg tcgtgcagca gtcccatctc
     1921 acgcaggcgg cgcgtctccg gctcaggaag gccgagcgcg cg
//
LOCUS       DAOTIL010000538         1961 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminobacteriaceae bacterium isolate AMR_MDS_3026
            NODE_68917_length_1961_cov_2.738195, whole genome shotgun sequence.
ACCESSION   DAOTIL010000538 DAOTIL010000000
VERSION     DAOTIL010000538.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868050
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminobacteriaceae bacterium (wastewater metagenome)
  ORGANISM  Aminobacteriaceae bacterium
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae.
REFERENCE   1  (bases 1 to 1961)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1961)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:19:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,224
            CDSs (total)                      :: 3,198
            Genes (coding)                    :: 3,184
            CDSs (with protein)               :: 3,184
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1961
                     /organism="Aminobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_68917_length_1961_cov_2.738195"
                     /isolate="AMR_MDS_3026"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:3034609"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1242)
                     /locus_tag="P5201_10515"
     CDS             complement(<1..1242)
                     /locus_tag="P5201_10515"
                     /inference="COORDINATES: protein motif:HMM:NF024589.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HRV99016.1"
                     /translation="MLTANLIGPPRFFLDDRELFFPIRKAQALLCYLLVEERASREAL
                     SSLLWGGSEDMLASQSLRNALYLLRKGLPEGLVGTGRQWITVDMSRVDLDLLLLERLD
                     DAPLERLPALCRPLLEGFSLPNAPEFDDWLRTARYQWAGRIRSAFLALSRRRMEAEEW
                     EAALCPLNLVAAGDEIDEESYRLIMRAHAARREWGRVAEAWRVLEGRLKNELGVEPSS
                     ESLALAACLLKKRDTRTQVFEKTQPAPRKDFFFGRSSELRSVREFCGAVSASPRCIFV
                     EGEAGVGKSLFVRTALPLSFPAEPLILRCTAGNPEAAYPLLPWDELLSSLFRSVDRES
                     LALPQTLWTILGQSFPSIGSRKTSVQPRNPRRLGAMLGDLFASAGATRPLALVFEDFQ
                     WFDEPSMDVLEHLLRSSPGNLT"
     gene            1468..>1961
                     /locus_tag="P5201_10520"
     CDS             1468..>1961
                     /locus_tag="P5201_10520"
                     /inference="COORDINATES: protein motif:HMM:NF020897.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HRV99017.1"
                     /translation="MIKSEHDSKQDGAFKVTAIMEALCASSSSLTVREIEERTGIPRS
                     TAHRFLVSLEERRWVFSDAATEGYRPGIRFFHLSSKSSFYDELVKKAEPGMFRLMTST
                     GNTAVLSVAEGSSGSCIHSVEPLSPVKFTAHRGMTIPLHAGATGKILLAFCSQEVRST
                     VLSSS"
BASE COUNT          480 a          596 c          526 g          359 t
ORIGIN      
        1 cgtgagattc cccggtgacg aacggagcag atgttcaagc acatccatcg aaggctcgtc
       61 aaaccattgg aagtcctcga acaccagcgc cagaggacgc gttgcgccgg ccgaagcgaa
      121 gagatcccca agcatggctc cgagacgacg ggggtttctg ggctggaccg acgtcttgcg
      181 gcttccgata gaagggaaac tctgccccag aatagtccag agcgtttggg gaagagcaag
      241 actctctctg tcgactgaac ggaagagaga cgacaggagt tcgtcccatg gaagcaaggg
      301 atatgcagcc tccggatttc cagcagtgca tcgcagtatt aacggctcgg ccgggaaaga
      361 aagagggagc gccgtcctga caaagagact cttgccgaca ccggcctctc cttcgacgaa
      421 aatgcatcgc ggcgacgcgc tcaccgcacc gcaaaattcc cgaacagagc gcagctctga
      481 ggaacgtcca aaaaagaagt ccttccttgg agcaggctgc gttttttcaa atacctgcgt
      541 tcgtgtatcc cgctttttaa gaagacacgc ggcgagagcc agactctcgc tcgacggctc
      601 cacgcccaac tcgttcttca ggcggccttc aagtaccctc cacgcctccg ccactctgcc
      661 ccactcgcgc cgcgccgcat gagcgcgcat tatcaggcgg tacgactctt cgtcaatttc
      721 gtcccctgcc gctacaaggt tcagcggaca cagcgcagcc tcccactcct ccgcctccat
      781 acgccgccga gacaatgcaa gaaaagcgga gcgaattctt ccggcccact ggtagcgtgc
      841 agtcctgagc cagtcatcga actccggcgc gttcggcagc gaaaatccct caaggagcgg
      901 acggcataaa gcgggaagac gctcgagagg ggcgtcgtca aggcgttcca aaagaagcag
      961 atcaaggtcc acacgggaca tatctacggt aatccactgc cggcccgttc ccacgagacc
     1021 ctcggggagg cctttccgaa gaaggtagag cgcattcctc aggctctggg aagcaagcat
     1081 gtcttcgctc ccgccccaga gcaacgacga gagcgcctca cgagaagctc tttcctcgac
     1141 caggagatag caaaggagcg cctgggcctt ccggatcgga aagaacagct ctctgtcgtc
     1201 aagaaaaaac cgcggagggc ctatcaggtt ggcggtgagc atgaacggct cccctttccc
     1261 tcgggaaata cgaaattgtt actgaaaaac tatgaacgat tataccatcg acctcctccc
     1321 ggaacccagg aacaccggaa aaacaagaac tcaaaagatg aatggagcct ctcctcacac
     1381 gcccctgtcg ggaccctgcc gcagcgattg acaaaccggc acgctcatga taataatggg
     1441 acacattgtc ccattttgga ggtcgcgatg ataaaaagcg agcacgattc taagcaggac
     1501 ggggcattca aagtgacggc aatcatggag gcgctctgcg catcctcttc gtcgctcact
     1561 gttcgcgaga tcgaggaacg gacaggaata cccagaagca cagcccacag gtttctcgtc
     1621 tctcttgaag aacgccgttg ggtcttcagc gacgctgcaa ccgaggggta ccggccgggc
     1681 atccgtttct ttcatctcag cagcaaatcc tctttctacg acgaactcgt taaaaaagca
     1741 gagccaggga tgttccgtct tatgacaagc acgggcaata ctgcggtcct gagcgtcgcc
     1801 gaaggatcct cagggtcgtg catccactcg gtcgagcctc tctcgccggt aaagttcacc
     1861 gcacaccggg ggatgacgat tcctcttcac gcaggagcca cggggaaaat actactcgcc
     1921 ttctgttctc aagaggtgcg atccacagta ctttcctctt c
//
LOCUS       DAOTIL010000539         1958 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminobacteriaceae bacterium isolate AMR_MDS_3026
            NODE_69034_length_1958_cov_3.564898, whole genome shotgun sequence.
ACCESSION   DAOTIL010000539 DAOTIL010000000
VERSION     DAOTIL010000539.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868050
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminobacteriaceae bacterium (wastewater metagenome)
  ORGANISM  Aminobacteriaceae bacterium
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae.
REFERENCE   1  (bases 1 to 1958)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1958)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:19:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,224
            CDSs (total)                      :: 3,198
            Genes (coding)                    :: 3,184
            CDSs (with protein)               :: 3,184
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1958
                     /organism="Aminobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_69034_length_1958_cov_3.564898"
                     /isolate="AMR_MDS_3026"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:3034609"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..288)
                     /locus_tag="P5201_10525"
     CDS             complement(<1..288)
                     /locus_tag="P5201_10525"
                     /inference="COORDINATES: protein motif:HMM:NF012799.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent methyltransferase"
                     /protein_id="HRV99018.1"
                     /translation="MLCIVPTPVGNLEDMTPRGVRMLAEADFIACEDTRTSMPLLRRY
                     GISKPLIAYHAFNEKGKTEALVERLLRGEKGAPISDAETPGISDPGYELIRR"
     gene            complement(276..1058)
                     /locus_tag="P5201_10530"
     CDS             complement(276..1058)
                     /locus_tag="P5201_10530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014162786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HRV99019.1"
                     /translation="MSAPENATHPVSRDELLMGSLSLDQPEKGPRVNADTILLASYVR
                     NTFSGARGGSDVLEIGCATGAVSLILAYRFPEIPRIVGVDIQGRLVQLAAANAVRNGF
                     ADRVSFIEADVRAARSSFPAQSFDVVAANPPYESPGRGRLSATHSERLARQEVSCTLE
                     EIAAACRYLLRNRGRLYLVFKAERVCELFVRLSEQGIEPKRVRWVHPLPGRKASVVLV
                     EALRGGRPGMIVEPPLFIHDATGGYTEEFLSAYTKEGLPCCV"
     gene            complement(1055..>1958)
                     /locus_tag="P5201_10535"
     CDS             complement(1055..>1958)
                     /locus_tag="P5201_10535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012869943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRV99020.1"
                     /translation="LIETWEEEYPYDAASAPGPGEGEAIASAVRRLERDLRALGPVDW
                     GALSEDTSLASRVAYLGEQLADVRAAMDELRAIIAETDRHVGVVFTGALDNINVRFDA
                     LFRRLFGGGEARLQLLSPPQPGQPDANGEDEAQPEQGESAAAWDRGVEIVARPPGKHL
                     QNLAQLSGGEQTLTAIAYLFASMEVAGVPLAVLDEVDAALDESNLLRFGELAREYACP
                     SEEASNADAEDAGRAASSSAIQLIVMTHRRATMERADILYGVTLAEPGLSKVVGIRVE
                     DWVEPGTDPRGRRAPTAVSLRGRP"
BASE COUNT          325 a          658 c          643 g          332 t
ORIGIN      
        1 cctgcggatg agttcgtacc cggggtcgga gatcccgggc gtctccgcgt cggagatagg
       61 agcccccttc tctccgcgga ggaggcgttc gacgagcgcc tccgtcttcc ccttctcgtt
      121 gaaggcatgg tacgcgatga ggggcttcga gatgccgtag cggcgcagca gcggcatcga
      181 ggttcgcgta tcctcgcagg cgatgaagtc cgcctcggcg agcatacgaa cgcccctcgg
      241 cgtcatgtcc tcaaggtttc ccaccggggt ggggactata cacagcacgg caatccctcc
      301 ttcgtgtagg cgctcagaaa ctcctccgta tatccgcccg tggcgtcgtg aataaagagc
      361 ggcggctcga cgatcatgcc cggacgcccg ccccggagcg cctcgacaag caccacggag
      421 gcttttcttc cggggagtgg atggacccag cgcactcgtt tcggctcgat cccctgttcg
      481 gagagacgga caaaaagttc acagacacgc tccgccttga agacgaggta gagcctcccc
      541 cggttcctga gaaggtaacg gcaggccgcg gcgatctcct caagcgtgca cgagacctcc
      601 tgcctcgcaa gccgttcgga gtgcgtcgcg gagaggcgcc ctctcccggg gctctcgtac
      661 ggcgggttcg ccgccacaac gtcgaacgac tgcgcgggga aggacgagcg ggcggcccgg
      721 acgtctgcct cgatgaagga aactctgtcc gcgaagccgt ttctgaccgc gtttgctgcg
      781 gcaagttgga cgagtctccc ctggatgtcc accccgacga tccggggaat ttccgggaag
      841 cggtacgcaa gaatgagaga gacggccccc gtggcgcagc cgatttcaag gacgtcgctt
      901 cctccgcgcg cccctgagaa cgtattgcgc acgtaggatg cgaggagaat ggtgtctgca
      961 ttgactctcg gccccttctc cggctggtcc agcgagagcg agcccatcag gagctcatcc
     1021 cgcgaaaccg gatgggttgc gttttcagga gcgctcacgg ccgcccccgg agggaaacag
     1081 ccgtaggcgc gcggcgtccg cgaggatccg tccccggctc cacccagtcc tcgacgcgta
     1141 tgccgaccac tttcgagagt cccggctccg cgagcgtcac gccgtagaga atgtcggccc
     1201 gctccatggt cgccctccgg tgcgtcatca cgatgagctg aatcgcggag gatgaggccg
     1261 cacggccggc gtcctccgcg tcggcgttcg acgcctcctc cgaaggacag gcgtactccc
     1321 gggccagctc gccgaagcgc agcaggttgg attcgtcgag cgccgcgtcc acttcgtcaa
     1381 gaacggcaag aggaaccccc gcgacctcca tcgaggcgaa gaggtaggcg attgcggtca
     1441 gcgtctgttc gcccccggag agctgggcaa gattctgcag atgctttccg ggagggcgtg
     1501 cgacgatttc gacccctctg tcccatgccg cggcgctttc cccctgctcg ggctgcgctt
     1561 cgtcctcccc gttcgcatcg ggctgtccgg gctggggcgg agaaaggagc tgcaagcgcg
     1621 cctctcctcc tccgaagagc ctgcggaaga gcgcgtcgaa acgaacgttg atgttgtcga
     1681 gggcgcccgt gaacacgacg ccgacatgcc ggtccgtctc ggcgatgatg gcgcgcagtt
     1741 cgtccatcgc cgcccggacg tcggcgagct gttctcccag gtacgcgacg cgcgacgcaa
     1801 gcgacgtgtc ttccgagagc gctccccagt cgacgggacc gagcgcgcgg agatcgcgtt
     1861 cgagcctgcg gacggcggag gcgatcgcct cgccttcgcc gggtccgggc gcggaggccg
     1921 cgtcgtacgg atactcctcc tcccacgtct cgatcagg
//
LOCUS       DAOTIL010000540         1952 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminobacteriaceae bacterium isolate AMR_MDS_3026
            NODE_69300_length_1952_cov_3.281497, whole genome shotgun sequence.
ACCESSION   DAOTIL010000540 DAOTIL010000000
VERSION     DAOTIL010000540.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868050
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminobacteriaceae bacterium (wastewater metagenome)
  ORGANISM  Aminobacteriaceae bacterium
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae.
REFERENCE   1  (bases 1 to 1952)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1952)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:19:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,224
            CDSs (total)                      :: 3,198
            Genes (coding)                    :: 3,184
            CDSs (with protein)               :: 3,184
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1952
                     /organism="Aminobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_69300_length_1952_cov_3.281497"
                     /isolate="AMR_MDS_3026"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:3034609"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..750
                     /locus_tag="P5201_10540"
     CDS             <1..750
                     /locus_tag="P5201_10540"
                     /inference="COORDINATES: protein motif:HMM:NF014073.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter permease"
                     /protein_id="HRV99021.1"
                     /translation="AFIGTFVGILPGVGGSAASLLSYSQAKNFSGRPEKYGTGCVDGL
                     VASEAANNGLTGGALIPLLSLGIPGDSTTAVLVGAFMLQGVQVGPLFITNNLDLWHVI
                     LSALLLCNFLMFFVMFYPIKYIASVIKLPKYRIFPVIILLCVIGAYSSRNGNMFDVWT
                     MLFFGLLGYFFVKIKLPIVPFLIGFILGKDLEKYFVDSIKGVGGSLSVFFTRPMGWVI
                     WGLIVASIAWAVYDSKREARRAAAGNGGAQA"
     gene            747..>1952
                     /locus_tag="P5201_10545"
     CDS             747..>1952
                     /locus_tag="P5201_10545"
                     /inference="COORDINATES: protein motif:HMM:NF016203.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UxaA family hydrolase"
                     /protein_id="HRV99022.1"
                     /translation="MIPVIRVDERDNVAIPVADLPEGSEAGWGVVAREQIPQGHKIAL
                     EEIPEGGAVIRYGVALGHLLAGVPQGGLITEKMLRLPDSPDLDSLEFATDIDAELPEA
                     PVKSFEGFEVEGSLFAGTRNILGIMPTVQCVAGVLNGAVKRMRRELLPRFPHVDDIVA
                     LNHPYGCGVAIDAPEAVVPIRTLRNILRNPNFGGEIMVVGLGCEKLTADRLLEPEEIR
                     PGNVLMLQEHAGFVKMTDALMEMAERKLRRLEGRRRTTLPLSRLCVGMQCGGSDAFSG
                     ITANPAAGYASDLLVGAGATVLFSEVTEVRDGVEQLAARCVNSAVGRRLVEEMRWYDE
                     YLRAGGVDRSANPTPGNKKGGLANIVEKAMGSIAKSGRAPISEALAPGERPSAGGLVF
                     AATPASDMVC"
BASE COUNT          318 a          608 c          656 g          370 t
ORIGIN      
        1 gcgttcatcg gcaccttcgt cggcatcctt cccggagtgg gggggagcgc ggcgtcgttg
       61 ctctcgtatt cgcaggcgaa aaatttctcc gggcgccctg agaagtacgg cacgggctgc
      121 gtcgacgggc tggtcgccag cgaagccgcc aacaacggac tgacgggagg cgcgctcatt
      181 ccgctgctct ccctcggcat ccccggggac agcaccacgg cggtgctcgt cggcgccttc
      241 atgcttcagg gagtgcaggt cgggccgctc ttcatcacga acaacctcga cctctggcac
      301 gtcatcctct ccgcgcttct tctgtgcaac tttctgatgt ttttcgtcat gttctaccct
      361 atcaagtaca tcgcttcggt catcaagctt cccaagtaca ggatattccc cgtgatcatc
      421 ctcctctgcg tcatcggtgc gtattcttcg cggaacggaa acatgttcga cgtgtggacc
      481 atgctctttt tcggcctcct ggggtatttc ttcgtgaaga tcaagctccc gatcgttccc
      541 tttctcatcg gcttcatcct cgggaaggat ctggagaagt acttcgtcga ctccatcaag
      601 ggggtcggcg gctccctctc ggtctttttc acgcgcccga tggggtgggt gatctggggg
      661 ttgatcgtcg cctccatcgc gtgggcggtt tacgactcga aaagagaggc ccggcgcgcc
      721 gcggccggaa acggaggcgc gcaggcatga taccggtcat cagggtggac gagagggaca
      781 acgtcgccat ccccgtcgcc gaccttcccg aaggttcgga ggccggatgg ggtgtggtcg
      841 cccgtgaaca gatcccccag gggcacaaga tcgctctgga agaaatcccc gagggtggcg
      901 cggtgatccg gtatggcgtg gcgctcgggc atctgctcgc aggcgttccg cagggcggac
      961 tgatcaccga gaagatgctt cgtctgcctg actctcccga cctcgatagt cttgagttcg
     1021 ccaccgacat tgacgcggag cttcccgagg cgcccgtgaa gagctttgag gggttcgagg
     1081 tcgagggctc cctcttcgcc ggtacgcgga acattctcgg catcatgccc acggtccagt
     1141 gcgtcgcggg cgtgctcaac ggcgcggtca agcggatgcg gagggagctg cttccgcgct
     1201 tcccgcacgt ggacgacatt gtggcgttga accaccccta cggctgcggg gtcgccatcg
     1261 acgcccccga ggccgtcgtt cccatcagga cgctgcggaa catactgcgg aacccgaact
     1321 tcggagggga gatcatggtc gtcggactgg ggtgcgagaa gctcaccgcg gacaggctgc
     1381 tcgaaccgga ggagatccgc cccgggaatg tgctgatgct ccaggagcac gccgggttcg
     1441 tcaagatgac cgacgccctg atggagatgg cggagcggaa gctccgcagg ctggagggca
     1501 ggcgcaggac gacgctgcct ctctcgcgtc tctgcgtcgg gatgcagtgc ggagggagcg
     1561 acgccttttc cggcattacc gcgaacccgg cggccgggta cgcgtcggac cttctggtcg
     1621 gggcgggcgc gacggtgctc ttttccgagg tcaccgaagt gcgggacggc gtggagcagc
     1681 tcgctgcgcg ctgcgtgaac tccgccgtgg ggaggcggct cgtcgaagag atgcgatggt
     1741 acgacgagta cctccgtgcg gggggcgtgg accgcagcgc caaccccact cccggaaaca
     1801 agaagggcgg gctggcgaac atcgtcgaga aggcgatggg ctcgatcgcc aagtcgggac
     1861 gagccccgat ttcggaggcg ctcgcccccg gagagcgtcc gtccgccggg ggactcgtct
     1921 tcgccgccac gcccgcgagc gacatggtct gc
//
LOCUS       DAOTIL010000541         1947 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminobacteriaceae bacterium isolate AMR_MDS_3026
            NODE_69542_length_1947_cov_3.494715, whole genome shotgun sequence.
ACCESSION   DAOTIL010000541 DAOTIL010000000
VERSION     DAOTIL010000541.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868050
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminobacteriaceae bacterium (wastewater metagenome)
  ORGANISM  Aminobacteriaceae bacterium
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae.
REFERENCE   1  (bases 1 to 1947)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1947)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:19:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,224
            CDSs (total)                      :: 3,198
            Genes (coding)                    :: 3,184
            CDSs (with protein)               :: 3,184
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1947
                     /organism="Aminobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_69542_length_1947_cov_3.494715"
                     /isolate="AMR_MDS_3026"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:3034609"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(86..364)
                     /locus_tag="P5201_10550"
     CDS             complement(86..364)
                     /locus_tag="P5201_10550"
                     /inference="COORDINATES: protein motif:HMM:TIGR03793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NHLP leader peptide family RiPP precursor"
                     /protein_id="HRV99023.1"
                     /translation="MDRKEMYKATGKLIAKAWVDAQFKAKLMADPASVLRAEGFAIPE
                     GMRVDVLENTDERIYFVIPKKPDQLSDEELDIAAAEGQNDVFCSCDFY"
     gene            592..>1947
                     /locus_tag="P5201_10555"
     CDS             592..>1947
                     /locus_tag="P5201_10555"
                     /inference="COORDINATES: protein motif:HMM:TIGR03605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SagB family peptide dehydrogenase"
                     /protein_id="HRV99024.1"
                     /translation="MHEEARRAGTEKNAPAGEGKTSAPIFDPESLAVSLKRGVRLHET
                     DGRAVLVDADGVALALPLSPETRRIFLSALDEGVCTADELGDAALHAAPSTDAASLYY
                     VLAQLERGRWFRYSLRSDGRNTATLEPFSRRFEFADAPLTCRYWLSRFACLRREGDDV
                     ALESPLGHGRILLHTSETAALAASLSRPLSFEEMCVSHPALAPKDIGALLAMMRSVGA
                     LFPCDAEGRIPEDGSEQLRQWEFYDLLFHSRSRQGRHGNPIGGSCPFLGEILPLPGVK
                     RPISAERVPLHRPETGERRADFFELAEMRRSLRTQGERALRLKELGTFLHFTGGVREI
                     LPASPEKGYWYEASLRPCAGSGAIHELEYYLTVARCDGLPPGLYRYDPLAHALERLEA
                     DEKQLEKLLKGAARAMRGARCDVLITLAARFQRVSWQYRSIAYSLLLKDVGAVFQQMY
                     LA"
BASE COUNT          380 a          587 c          596 g          384 t
ORIGIN      
        1 gtccataccg tacccctccc gttaaaaaaa gtagcgcttc ggcgcgcgtc cgccgctcat
       61 gggcggacgg gcataccatg aaggctcagt agaagtcgca cgagcagaag acgtcgttct
      121 gcccctcggc cgcggcgata tccaactcct cgtcagagag ttggtcgggc ttcttcggga
      181 taacaaagta tatgcgctcg tccgtgtttt cgaggacatc gacccgcatt ccctccggaa
      241 tcgcgaatcc ctcggctcgc agaaccgacg cgggatcggc catcagcttc gccttgaact
      301 gcgcgtcaac ccacgccttg gcgatcagct ttcccgtcgc cttgtacatc tcttttctgt
      361 ccattttcgt tcaccctcca cactgaaatt ttagaaatcc gagctattct gaattataaa
      421 ctctgacata gtgtgagagt caaggctgct ttatgctttt tttcgaaaaa atgataaagt
      481 ggtgtccaaa gaaagaccgc ttcgatgaac acactgcctc tgcatcggcg agcgagcgga
      541 ccggacagac gaaaagaaaa acgggcaaga gaaaagggtg aaataacgaa aatgcacgaa
      601 gaagcgcgcc gcgcaggcac tgaaaaaaat gctcccgccg gggagggaaa aacatccgcc
      661 ccgatcttcg acccggaatc tctcgcagtc tctttgaaga gaggcgtccg tctgcacgaa
      721 acggacggac gcgccgtact tgtcgacgcg gacggcgttg cgctcgccct tccgctttct
      781 cccgaaacgc ggcggatttt cttgtcggcg ctggacgagg gagtatgtac cgcggacgag
      841 ctgggggacg cggcgcttca cgccgcgcct tcaaccgacg cagcctctct gtactacgtt
      901 ctggcgcagc tcgaacgggg acggtggttc cggtactcgc tgcgctccga cgggcgaaat
      961 acggcgacgc tggaaccgtt ctcccgccgc ttcgaatttg cggatgcccc gcttacatgc
     1021 cgttattggc tgtcccgctt cgcctgtctg cggcgcgagg gagacgacgt cgctctggag
     1081 agtccgttgg ggcacggacg gatactgctg cacacatcag agactgccgc cctcgcagca
     1141 tccctctctc gcccgctgtc gttcgaagag atgtgcgttt cgcatcccgc gcttgcccca
     1201 aaggatatcg gagcgctgct cgcgatgatg cgcagcgtcg gtgcgctctt cccgtgcgac
     1261 gcggagggac ggattccgga ggacgggagc gaacagctgc gtcagtggga gttttacgat
     1321 cttctcttcc atagccgcag caggcaggga agacacggca atcccatagg aggatcctgt
     1381 ccgttcctcg gggaaattct cccgctgccg ggagtgaagc gtccgatatc ggcggagcgc
     1441 gttcctcttc accgcccgga gacgggggag aggcgcgcgg atttcttcga actcgcggag
     1501 atgcgccgtt cgctgagaac gcagggggag cgggcgctcc gcctgaagga actggggacg
     1561 ttcctgcact tcacgggggg cgttcgggaa atactgcccg cctccccgga gaaggggtat
     1621 tggtacgagg cttcgctgcg cccttgcgcg ggaagcgggg cgatccacga actggagtac
     1681 tacctgacgg tcgccaggtg cgatgggctt ccgccggggc tgtaccggta cgatccgctc
     1741 gcccatgcgc tcgaacgact cgaagcggac gaaaaacagc tggaaaagct cttgaagggc
     1801 gcggcccgcg ccatgagggg agcgcgctgc gacgtgctga tcactctggc ggcgcggttt
     1861 cagcgcgttt cgtggcagta tcgctccatc gcctactcgc ttcttctgaa ggatgtcggc
     1921 gccgtcttcc agcagatgta cctcgcg
//
LOCUS       DAOTIL010000542         1944 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminobacteriaceae bacterium isolate AMR_MDS_3026
            NODE_69725_length_1944_cov_2.757014, whole genome shotgun sequence.
ACCESSION   DAOTIL010000542 DAOTIL010000000
VERSION     DAOTIL010000542.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868050
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminobacteriaceae bacterium (wastewater metagenome)
  ORGANISM  Aminobacteriaceae bacterium
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae.
REFERENCE   1  (bases 1 to 1944)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1944)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:19:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,224
            CDSs (total)                      :: 3,198
            Genes (coding)                    :: 3,184
            CDSs (with protein)               :: 3,184
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1944
                     /organism="Aminobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_69725_length_1944_cov_2.757014"
                     /isolate="AMR_MDS_3026"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:3034609"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..267)
                     /locus_tag="P5201_10560"
     CDS             complement(<1..267)
                     /locus_tag="P5201_10560"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRV99025.1"
                     /translation="MGEPSDEATVFGTRAGTPFPKNTPECSIKHSIGRLRSNQRAFAR
                     AFLGRFAEPALLCALRSPPRERELPDGRLGEIVEAGDIGEVVRVA"
     gene            290..1138
                     /locus_tag="P5201_10565"
     CDS             290..1138
                     /locus_tag="P5201_10565"
                     /inference="COORDINATES: protein motif:HMM:NF012546.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HRV99026.1"
                     /translation="MLKRIISNRRARYALLFVALLYGAALLGVRRLVYRPWANFSRTP
                     DAYGWEWEDVRFRAKDGATLHGWYIPGDSHRTVLYCHGNAGNISHGLRRADILKSLGV
                     SVFLFDYRGFGESSGRPTESGLYADTEAALDWLQEHKGLSGTDVAVFGRSLGGAAALE
                     LAVRRPVAGVLLESTFTSLADVARAFVPYLPAELFVFNAFDNMRKIERINVPVLIMAS
                     IDDEITPFEHGQRLFEEAAEPKFFLTMNGRHRSHFYRTEQEYVDAIRDFLEFVFGAEQ
                     PDLQES"
     gene            complement(1167..>1944)
                     /locus_tag="P5201_10570"
     CDS             complement(1167..>1944)
                     /locus_tag="P5201_10570"
                     /inference="COORDINATES: protein motif:HMM:NF019581.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HRV99027.1"
                     /translation="DWIRYQGLKIFLDGSLGGRTAALTAPYADEPAQTGRLNWTDEQV
                     LGMLRAARGKGIQTLLHAIGDRALDQALDCIGKVDEEFGPAALKDRVNHLIVCRPEQR
                     RRLAELGLFCDIQPSFIPSDIAMAPLRLGPERMAWTYRWRSILNECGPLSASSDAPVE
                     SVNPMHSLWALVERRGKDGEAPLSPEERLSVEEALPLFTSNPYKAVGRSDAGRLEAGC
                     LADVVVLDRDLHGLSGDDLYSVGVRCTIAGGRATHEEMEA"
BASE COUNT          326 a          619 c          657 g          342 t
ORIGIN      
        1 ggcgactcgg acgacttcgc cgatatcgcc tgcctcgact atttctccga gacgtccgtc
       61 ggggagttcg cgctctcggg gcggtgaacg aagggcgcaa agaagcgccg gttccgcaaa
      121 acggccgagg aacgcgcgcg caaaagcgcg ttgattcgac cgcagccttc cgatagaatg
      181 ctttatggaa cactcagggg tgttcttcgg gaaaggcgtt ccggcccggg tcccgaaaac
      241 cgtcgcctca tcggagggtt cgcccatatg acccgaaagg aaagaataca tgctgaaacg
      301 aatcatttca aaccggcgcg cgcgctacgc gctcctcttc gtcgcgctgc tctacggcgc
      361 ggcccttctg ggcgtgcggc gcctcgtcta ccgtccgtgg gcgaactttt cccgcactcc
      421 ggacgcgtac ggctgggagt gggaggacgt ccggttccgg gcgaaggacg gcgcgacgct
      481 ccacggatgg tacattcccg gcgattcgca cagaacggtg ctttactgcc acggaaacgc
      541 ggggaacatc tcccacgggc tgcggcgcgc ggatattctg aagtcgctgg gcgtgtcggt
      601 cttcctgttc gactacaggg ggttcggcga aagctccggc agaccgacgg agagcgggct
      661 ctacgccgat acggaggccg ctcttgactg gctgcaggag cataaggggc tttcgggaac
      721 ggatgtagcg gtcttcgggc gctcgctggg cggcgctgcg gcgctggagc tggcggtgcg
      781 ccgcccggtt gcgggcgtgc tcctcgaatc cacctttacg agcctcgcgg acgtggccag
      841 ggccttcgtg ccctaccttc ccgccgaact gttcgtcttc aatgcgttcg acaacatgcg
      901 gaagatagaa cgcatcaacg tgcccgtcct gataatggcg agcatcgacg acgagatcac
      961 cccgttcgaa cacggacagc gcctcttcga agaggccgcg gagcccaagt tctttttaac
     1021 gatgaacggg cggcaccggt cgcacttcta ccgcacggaa caggaatacg tcgacgcgat
     1081 ccgggacttt ctggagttcg tcttcggagc ggagcaaccc gaccttcaag aaagctgaac
     1141 ggcgtcccga ccgtgagtgc gggtatctac gcctccattt cttcgtgcgt cgcgcgccct
     1201 cccgcgatgg tgcagcgtac gccgacggag tataggtcgt cccccgagag gccgtgcaga
     1261 tccctgtcga gcacaacgac gtcggccaga caccccgctt cgaggcgtcc ggcgtcgctt
     1321 ctgccgaccg ccttgtacgg attcgacgtg aagagcggaa gcgcctcttc gacggacagc
     1381 cgctcctcgg gcgagagcgg cgcttcgccg tccttcccgc gccgttcgac gagcgcccag
     1441 agcgaatgca tcgggttgac cgactcgacg ggggcgtcgc tggaggcgga gagcggcccg
     1501 cactcgttga ggatggagcg ccaccggtag gtccacgcca tccgttccgg gccgaggcgc
     1561 agcggcgcca tggcgatgtc gctggggatg aacgagggct ggatgtcgca gaagagcccc
     1621 agttcggcga gacggcggcg ctgctccgga cggcagacga tgaggtggtt gaccctgtcc
     1681 ttcagcgcgg cggggccgaa ctcttcgtct acctttccga tgcagtcgag cgcctggtcg
     1741 agcgcccggt cgccgatggc gtggaggagc gtctgaatcc ccttcccgcg cgcggcgcgg
     1801 agcatcccga ggacctgctc gtccgtccag ttcagccgtc cggtctgggc cggttcgtcc
     1861 gcgtacgggg cggtgagcgc ggcggtgcgc ccgccgaggc tgccgtcgag gaagatcttg
     1921 agcccctggt aacggatcca gtcg
//
LOCUS       DAOTIL010000543         1941 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminobacteriaceae bacterium isolate AMR_MDS_3026
            NODE_69868_length_1941_cov_3.025451, whole genome shotgun sequence.
ACCESSION   DAOTIL010000543 DAOTIL010000000
VERSION     DAOTIL010000543.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868050
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminobacteriaceae bacterium (wastewater metagenome)
  ORGANISM  Aminobacteriaceae bacterium
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae.
REFERENCE   1  (bases 1 to 1941)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1941)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:19:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,224
            CDSs (total)                      :: 3,198
            Genes (coding)                    :: 3,184
            CDSs (with protein)               :: 3,184
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1941
                     /organism="Aminobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_69868_length_1941_cov_3.025451"
                     /isolate="AMR_MDS_3026"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:3034609"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1941)
                     /gene="secA"
                     /locus_tag="P5201_10575"
     CDS             complement(<1..>1941)
                     /gene="secA"
                     /locus_tag="P5201_10575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013048806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006605 - protein targeting [Evidence IEA];
                     GO_process: GO:0006886 - intracellular protein transport
                     [Evidence IEA];
                     GO_process: GO:0017038 - protein import [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="preprotein translocase subunit SecA"
                     /protein_id="HRV99028.1"
                     /translation="EVSRRTIGLRHFDVQLMGGMALHEGKITEMKTGEGKTLVATLAV
                     VLNALSGEGVHLVTVNDYLARRDAEWMGPIYTFLGLSVGVIYAYMDPEERLASYRADI
                     TYGTNSEFGFDYLRDNMAVNESHLVQRGHAYCIVDEVDSILIDEARTPLIISGPSDDN
                     VEMYTTSDRIARQLKEGRDFEKDEKERNVAMTEAGIARCEELLKLPDLFSDAAHSDLA
                     HRIVQALKARILFQKDVHYVVKDGEIIIVDEFTGRLMVGRRYSDGLHQAIEAKEGVKV
                     GRESQTLATITLQNYFRMYRKLAGMTGTAATEAEEFKEIYGLEVIVVPTNVEMIREDC
                     PDVIYRTVLEKFSAAADEIEESYKEGCPVLVGTTSIESSERLSKLLKARKIPHQVLNA
                     KHHEREAQIVAQAGHLGAVTVATNMAGRGTDILLGGNPEFLTREALRRENGEAAESSE
                     QYSALLEKYREQCRAEREKVKALGGLKILGTERHESRRIDNQLRGRAGRQGDPGSSRF
                     YLSLEDDLLRLFGSERIQGLMEKLGMEEGESIEHNLLTKAIANAQKKVEQLHFDIRKQ
                     LLAYDNVMNRQREAVYTERQQILRDEGIQEHVWEIIEGVIDDILERYFPEEGEADPAR
                     AVARLKAIFGSGLEARLEGVDN"
BASE COUNT          373 a          595 c          504 g          469 t
ORIGIN      
        1 cgttatcaac accttccagc cgggcctcaa gcccgcttcc gaagatagcc ttcagacggg
       61 caaccgcgcg tgccggatcc gcttcccctt cctcggggaa gtaccgctca aggatatcgt
      121 cgatcacacc ttcgataatt tcccagacat gctcctggat cccctcgtcg cgaaggatct
      181 gctggcgttc ggtatagacg gcttctctct ggcggttcat cacgttgtca tacgccaaga
      241 gctgttttcg aatatcgaaa tggagctgtt ccactttttt ctgagcattt gcaatcgctt
      301 tggtgaggag attgtgctct atcgactctc cctcctccat tccgagcttc tccatgagcc
      361 cctgaatacg ttcggagccg aaaagacgga gaagatcgtc ttcaagcgaa agatagaagc
      421 ggctggagcc aggatcgccc tgacgtcccg cgcgacctcg aagctgatta tcgatccgcc
      481 tggattcatg acgctcagtc cccagtattt tgagacctcc gagagccttt accttttcgc
      541 gttccgcgcg gcactgctca cggtatttct caagaagcgc agaatactgt tccgaactct
      601 ccgcggcttc tccgttctcc cggcgaaggg cttcccttgt caggaactcg ggattcccgc
      661 caagaagaat gtccgtgccg cgtcccgcca tattggtggc gacagtcaca gcgccgagat
      721 gccccgcttg ggcgacaatc tgcgcttccc gctcgtgatg cttggcattg agcacctgat
      781 gcgggatctt acgcgccttg agcagcttgc tcagacgctc cgagctctct atggatgtgg
      841 tgccaacaag gacagggcac ccttccttat atgattcttc tatctcgtcc gcagcggcgg
      901 aaaacttttc caaaacggtt ctgtagatga cgtccgggca gtcctcgcga atcatctcca
      961 cattcgtcgg gacaacaata acttcaaggc cgtagatctc cttgaactcc tccgcttcgg
     1021 tcgcggcggt tcccgtcatt ccggcgagct tgcggtacat gcggaagtag ttctgaagcg
     1081 ttatggtggc cagggtctgg ctctccctgc cgacttttac accctccttg gcttcgatgg
     1141 cctgatgcag tccgtcggag taacgccggc cgaccatcaa acggccggtg aattcgtcga
     1201 cgatgatgat ttctccgtcc ttcacgacgt agtggacatc cttctggaag agaatgcgcg
     1261 ccttcagggc ctgcacgatc cggtgcgcca gatcggagtg cgcggcatcg gaaaagaggt
     1321 ccggcagctt gaggagctcc tcgcatctgg cgataccggc ttcggtcatc gcaacgttcc
     1381 gctctttctc gtccttctcg aagtcgcgcc cctctttcaa ctgacgggct atccgatctg
     1441 atgtggtgta catctccacg ttgtcgtccg atggaccgga gatgatgagc ggcgttcgcg
     1501 cttcgtcgat caggatagag tcgacctcgt ccacgataca gtacgcgtgt cccctctgca
     1561 caagatgcga ctcgttcacc gccatgttgt cgcgcagata gtcgaaaccg aattcgctgt
     1621 ttgttccgta tgtgatatcc gctctgtagg aggcaagacg ctcttccggg tccatgtacg
     1681 cataaatgac gccgacggag agtccgagga atgtgtagat cggccccatc cactcggcgt
     1741 cccttctggc gaggtagtcg ttcaccgtca ccaggtgaac tccttcccct gaaagagcgt
     1801 ttaacacaac ggcaagagtt gcaacgagcg tttttccctc tcccgtcttc atctcggtga
     1861 tctttccctc gtgcagcgcc ataccaccca tcagctggac atcgaaatga cgcagtccaa
     1921 tggtgcgcct ggacacttct c
//
LOCUS       DAOTIL010000544         1940 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminobacteriaceae bacterium isolate AMR_MDS_3026
            NODE_69937_length_1940_cov_2.307162, whole genome shotgun sequence.
ACCESSION   DAOTIL010000544 DAOTIL010000000
VERSION     DAOTIL010000544.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868050
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminobacteriaceae bacterium (wastewater metagenome)
  ORGANISM  Aminobacteriaceae bacterium
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae.
REFERENCE   1  (bases 1 to 1940)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1940)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:19:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,224
            CDSs (total)                      :: 3,198
            Genes (coding)                    :: 3,184
            CDSs (with protein)               :: 3,184
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1940
                     /organism="Aminobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_69937_length_1940_cov_2.307162"
                     /isolate="AMR_MDS_3026"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:3034609"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..782
                     /locus_tag="P5201_10580"
     CDS             <1..782
                     /locus_tag="P5201_10580"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRV99029.1"
                     /translation="PTLALERDLQLMVLLDERIGDKRSFLSTAESETMRVFEKAGYLL
                     VDSTQASILKNIDLDAVRESADPEVFRQLARDFQADVLIYGKAYASSFANQVVSGVRI
                     YGVRSTVQLKAVLANTAYQLGSDAVEEKTKGVSVEDGAIKGLQPAAMKASGSIVHKIA
                     YTLISGSAGGIPGRTIKVKISNMTFKDVKELKESLQDVQGVTAVYQRLFRSKRLELDV
                     VSDMTAEEIASLLSDLGYDIEDLSSVTVEGVLNEKTGEKTP"
     gene            754..1263
                     /locus_tag="P5201_10585"
     CDS             754..1263
                     /locus_tag="P5201_10585"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRV99030.1"
                     /translation="MKKQVRRLHREKAPRPFRFFLVVCVCIATAGALTAAVPALFSGN
                     GDPGAEAQLVGEENPEMPRDPRIPETVNSGLLLREQGIATAEGVGRIPEDTAQGRLLG
                     RRAALTDARRNLLVLRQKLLDGKFDVRSVSGKIAEVRIYSEKTDGALYYLKVDVPLYR
                     LMKGDVPVE"
     gene            1256..1774
                     /locus_tag="P5201_10590"
     CDS             1256..1774
                     /locus_tag="P5201_10590"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRV99031.1"
                     /translation="MNKWTKFFTLAVMAGLLCLFGTSAFAELPKGSIAVLTKGPSVQH
                     SAAVASILTRELLAKGHKVVDPKRLDAIRRSKAAALALEGNADAIIKLSKQYGFSTMI
                     TAQLQAGKPVLNEFQLYTGTSSLAIMVMSSGGQMIYADTVMGKQVGYTPDEAAQKSIE
                     AAAKLAVQKMLQ"
BASE COUNT          435 a          527 c          612 g          366 t
ORIGIN      
        1 accccaccct cgccctcgag cgcgacctgc agctgatggt gctgctcgac gagcgaatcg
       61 gcgacaaaag atcttttctc tccaccgccg aaagcgaaac gatgcgcgtc ttcgagaagg
      121 ccggctattt gctcgtcgat tcgacgcagg cgagtatcct gaagaacatc gacctcgacg
      181 ccgttcgtga aagcgctgat ccggaggtct ttcgacaact ggcccgcgac ttccaggcgg
      241 atgtcctgat ctacgggaag gcctatgcca gttccttcgc aaaccaggtc gtcagcgggg
      301 tcagaatcta cggtgtgcga agcacggtcc agctgaaggc ggtgctggcg aacacagcat
      361 atcaactggg atcggacgca gtcgaggaga agacgaaggg cgtctccgtc gaggacgggg
      421 cgatcaaggg attgcagccc gccgccatga aagcgagcgg ctccatcgtg cacaagatag
      481 cctacacgct catttccggc tccgcaggcg gcataccggg aaggacgatc aaggtaaaga
      541 tctccaacat gacgttcaag gacgtaaaag agctcaagga gagcctccag gatgtccagg
      601 gggtgactgc cgtctatcag aggctgttca gaagcaagcg actcgaattg gacgtcgtgt
      661 ccgacatgac tgcggaggag atcgcctcct tgctctccga ccttggatac gatattgaag
      721 atttgtcgtc cgttacagtg gagggcgtcc tgaatgaaaa aacaggtgag aagactccat
      781 agagaaaaag cgccgcgtcc tttcaggttt ttcctcgtcg tgtgcgtctg catagcgact
      841 gccggagcgt tgacggcggc ggtcccggcg ctcttttcgg gaaacgggga tccgggcgcg
      901 gaggcgcagt tggtcgggga agaaaatccg gagatgccgc gggacccgag gataccggaa
      961 acggtgaact ccgggcttct cctccgtgag caggggattg ctacagcgga gggcgtcggc
     1021 cgcatcccgg aagatacggc tcagggaagg ctgctcggcc gaagggccgc gctgaccgac
     1081 gcgaggcgaa atctgcttgt gctacggcaa aaactgctcg atggaaagtt cgatgtacgg
     1141 tcggtgtcag ggaagatcgc cgaagtacga atttattcgg agaaaacgga cggagccctc
     1201 tactacctga aggtcgacgt tccgttgtac agactgatga agggggatgt tccggttgaa
     1261 taaatggaca aagtttttta cgcttgcggt aatggccggt ttgctgtgcc ttttcggcac
     1321 gagcgcattt gcggagcttc cgaaaggatc gatcgcagtg ctgacgaagg ggccgtcggt
     1381 tcagcattcg gccgccgtcg cctccatact gaccagggag cttctcgcga aggggcacaa
     1441 ggtagtcgat ccgaaacgcc ttgatgcaat ccgccgcagc aaggccgccg ctctggctct
     1501 cgaagggaat gcggacgcta tcatcaagtt gagcaaacag tacggtttca gcacgatgat
     1561 cactgcccag cttcaggcgg gaaagcccgt tctgaacgag tttcagctct acaccggaac
     1621 ctcgtccctc gcgatcatgg tgatgagttc gggcggccag atgatctacg ccgacacagt
     1681 gatgggaaag caggtcggat acacgccgga cgaagccgcg cagaaatcaa tagaggccgc
     1741 cgcgaagctc gcggtgcaaa agatgctcca atagtgcgga acgcgccgga gcgaaggaga
     1801 atctcatgaa aaagaaactt ttcgcgtgcg ttcttgcgat cgtctgcctc tcgatcttcg
     1861 cgggggctgc gctcgctgcg cccctctccc agaggccgag gctttccgtg cgccagttcg
     1921 acaacaaggc ggggtgcggt
//
LOCUS       DAOTIL010000545         1939 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminobacteriaceae bacterium isolate AMR_MDS_3026
            NODE_69982_length_1939_cov_2.867304, whole genome shotgun sequence.
ACCESSION   DAOTIL010000545 DAOTIL010000000
VERSION     DAOTIL010000545.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868050
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminobacteriaceae bacterium (wastewater metagenome)
  ORGANISM  Aminobacteriaceae bacterium
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae.
REFERENCE   1  (bases 1 to 1939)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1939)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:19:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,224
            CDSs (total)                      :: 3,198
            Genes (coding)                    :: 3,184
            CDSs (with protein)               :: 3,184
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1939
                     /organism="Aminobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_69982_length_1939_cov_2.867304"
                     /isolate="AMR_MDS_3026"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:3034609"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1474
                     /locus_tag="P5201_10595"
     CDS             <1..1474
                     /locus_tag="P5201_10595"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRV99032.1"
                     /translation="SLRKDIEIDPNDVEITESVEELEEIYSYPVATALYTTGEDEDMR
                     GNQDIFIFTDEWIFRVHLSLAADHMDDYDADEMKDWFEDMKIFDRDPSRANGSEDESQ
                     DRHATRADAGVEGLWLRIPDFPDDAEVLEFTADDDGEVTYIRALDDGALVLGIYRTPA
                     CDETTPEKVKESIVESVKDSGGDADDIEFDAGANDFSELLSYPCMTAEYKIGEDEDAK
                     RVAILGVFADDYVFLIQAAGAADTFGEYSDRSEEWFTHMKLVDGEEGKGDVFDDKTGK
                     DDEKTLDFVAVVYSLPEFRGIAWRIPAPGKYDLGDGFDIPNDSVCSVAVCPGYRVTLY
                     EHSQFGGETAEFEENEGDLEERSRWASSLKVERIEKPDFDLAFEWSMVQAENEGLFEE
                     INLDTAAAAKALEVHAERVERFKKAGEMHWYGTTTDAERVELGGELIKIFSDMGVATD
                     DWEADIFAQAMNNFYDWRKDLSVWDAACIILNVNPETFNQ"
     gene            1589..>1939
                     /locus_tag="P5201_10600"
     CDS             1589..>1939
                     /locus_tag="P5201_10600"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRV99033.1"
                     /translation="MKKVILYISLFCVFGAPFCFANAASAGDAPDAWVKGLWIQANGL
                     PEDASIEDYSDGDDEKPWFVYSWGEGGDGPAVTLAVGRFSASVDAEKKLQNLDKSVLR
                     EFIGSEDFWESSDAK"
BASE COUNT          505 a          479 c          600 g          355 t
ORIGIN      
        1 aagtctccgc aaggacatcg aaatcgaccc gaacgacgtc gaaatcaccg aatccgtcga
       61 ggaactcgaa gagatttact cgtacccggt cgcgacagcg ctgtatacga ccggcgaaga
      121 cgaggacatg agagggaacc aggacatttt catcttcacc gacgagtgga tcttccgcgt
      181 gcatctctcg ctcgcagccg atcacatgga tgactacgac gcggatgaaa tgaaagactg
      241 gttcgaggac atgaaaatct ttgatcgaga tccatcccgg gcaaacggaa gcgaagacga
      301 atcccaagac aggcatgcca cgagagcaga cgcgggtgtc gaagggctgt ggcttcggat
      361 tccggacttt ccggacgacg cggaagttct agagtttacc gcggacgacg acggtgaagt
      421 cacctacatt cgcgctcttg atgacggcgc actcgtgctt ggcatctaca ggacgcccgc
      481 ctgtgacgaa acaacgcccg aaaaagtaaa ggaatcgatt gtggagagcg tgaaggacag
      541 cggcggcgac gctgacgaca tcgagttcga cgcgggggcg aacgacttct ccgaactgct
      601 ctcgtacccg tgtatgacgg cggaatataa aatcggtgaa gacgaggacg ccaaaagggt
      661 cgcgatactc ggcgtattcg ccgacgatta cgttttcctc atccaagccg cgggcgcggc
      721 ggacacgttc ggcgagtact ccgacaggag cgaagagtgg ttcacacaca tgaagctcgt
      781 cgacggcgaa gaaggcaaag gcgatgtgtt cgacgacaaa accgggaagg acgacgagaa
      841 aacgctggat ttcgtcgccg tcgtctattc gcttccggag tttcggggca tcgcgtggcg
      901 catccccgcg ccgggcaaat acgacctggg cgacggattc gacattccca acgacagcgt
      961 gtgttccgta gcagtatgtc cggggtacag ggtgacactc tacgaacact ctcaattcgg
     1021 cggcgagacc gcggaattcg aggagaacga aggagacctc gaagagcgga gccgctgggc
     1081 gtcgtccctc aaagtcgagc ggatagagaa accggacttt gaccttgcct ttgaatggtc
     1141 tatggttcag gcggaaaacg aggggctctt cgaggagatc aatctagaca cagcggctgc
     1201 ggcgaaagcc ctcgaagtcc acgcggagag agtggagcgt ttcaaaaaag cgggtgagat
     1261 gcactggtac ggaacgacga cggacgcgga gcgtgtcgag ctgggcggag aactgataaa
     1321 aattttcagc gatatgggag tcgccacgga cgactgggaa gcggacattt tcgcgcaggc
     1381 gatgaacaat ttctacgact ggcgaaagga tttgagcgtg tgggatgcgg cgtgcataat
     1441 cctcaacgtc aacccggaaa cttttaacca atagggaaag agcgccggag agaaaaaaag
     1501 gaaacaggat acgcactgaa tacatgtttg gcaaacgaac ccccggaatt tggttcaaaa
     1561 ccatacatag attggaggtt tcagtgaaat gaaaaaggta atactgtaca tttcgctttt
     1621 ctgtgttttt ggcgcgccct tttgcttcgc gaacgccgcg agcgccggcg atgcaccgga
     1681 tgcatgggtg aaagggctat ggatacaagc gaacgggctg cccgaagacg cgtcgatcga
     1741 agactattcg gacggcgacg atgaaaaacc atggttcgtc tacagttggg gcgagggggg
     1801 cgacggccct gccgtaacgc tcgcagtggg gcggttttcg gcttcggtag acgcggagaa
     1861 aaaacttcag aacctcgaca aaagtgttct tcgcgaattt atcggctctg aggatttctg
     1921 ggaaagctcc gacgcgaaa
//
LOCUS       DAOTIL010000546         1938 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminobacteriaceae bacterium isolate AMR_MDS_3026
            NODE_70018_length_1938_cov_3.525757, whole genome shotgun sequence.
ACCESSION   DAOTIL010000546 DAOTIL010000000
VERSION     DAOTIL010000546.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868050
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminobacteriaceae bacterium (wastewater metagenome)
  ORGANISM  Aminobacteriaceae bacterium
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae.
REFERENCE   1  (bases 1 to 1938)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1938)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:19:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,224
            CDSs (total)                      :: 3,198
            Genes (coding)                    :: 3,184
            CDSs (with protein)               :: 3,184
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1938
                     /organism="Aminobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_70018_length_1938_cov_3.525757"
                     /isolate="AMR_MDS_3026"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:3034609"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            346..1887
                     /locus_tag="P5201_10605"
     CDS             346..1887
                     /locus_tag="P5201_10605"
                     /inference="COORDINATES: protein motif:HMM:NF016232.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative DNA binding domain-containing protein"
                     /protein_id="HRV99034.1"
                     /translation="MTKEELVARLKKPEWSDLECKRSRNGVSEDAYRTVSAFSNTEGG
                     SLVFGVKDSEGKLEIVGIDAPDKVQNDFLSALRTGDKLNRRINAEAERFEVENKILLV
                     FHIPEALRSEKPVYLRGDIRQSYIRRGAGDEQCLQTEIERFLRDAASERWDGLAIEYD
                     PDRCFDRRSLAWYRSRYESRPNNRSYAGVDDNEFLFQLGLIRETPQGRLPTHAAVLLF
                     GSDGYLRGMMPRPVADCQRFGFEFGENPPGVRWLDRVVMESNIVQAWLSFLDWYMKFA
                     SIPFRLDPTTMERDDTPADFIAFREAFVNVLCHQDYQDHTRKPEIRRFSDRTIFWNPG
                     DAFATGDLLAPGPKEVRNPTIAVAFRRIGFSENAGWGLNDVISQWTGLGYVRPVIRNG
                     KASKDFELMLIAASSPVSSGRIQPGEGVEQVTQQVTQQVTQQVTQQVEKLLAACTSEL
                     SRSELMKAVRIRDRVSFSRNYLEPALAESLIEMTQPDSPKSPTQKYRLTAKGRVLLES
                     VGSSK"
BASE COUNT          473 a          526 c          556 g          383 t
ORIGIN      
        1 tcccggacga gcgaccactt acgacggagc ggagagcgca cttcgggcaa acacaatcgg
       61 agtgcgccgc ctccgtgcta aaataatcgg aagttcgttt cggaaggggc tgccgatctt
      121 gaacgaacgg tccggacgaa atgggacaca taggcggaaa cagggcgtac gaaaagggag
      181 cggagcggga gatattctta ttgcacagga gaatacatgc aagcgtcgca ggtattcgca
      241 gaaaatccgc acgttaaaaa atgatttccg cacggtcgta ttggtccgcg tacagagtcg
      301 tgtagaggtg actcaatatt tctccgctaa cctggaggca actggatgac taaagaggaa
      361 ctcgtcgcca gactgaagaa acctgaatgg agcgatcttg aatgcaagag gtcgcggaac
      421 ggcgtttccg aagatgcgta ccggacggta tccgccttct caaatacaga gggcggcagt
      481 ctggtgttcg gcgtgaagga ctccgaaggc aaattggaaa tcgttggaat cgatgccccg
      541 gacaaggttc aaaacgactt tctcagcgcc ctgcggacag gagataaact gaatcgtaga
      601 ataaacgcgg aagcagaacg cttcgaagtc gaaaacaaga ttctgcttgt cttccatatc
      661 cccgaggcat tacgcagcga aaaacccgtc tatctcagag gagacatcag gcagagctat
      721 attcgccgcg gcgccggaga tgaacagtgc ctgcaaacgg agatcgagcg attcctgcgg
      781 gatgccgcgt cggagcgctg ggacggcctc gccatagagt acgatccgga ccgctgtttc
      841 gaccgccgtt cactggcctg gtatcgaagc cgttacgaaa gccggcccaa taaccgctcc
      901 tacgcggggg tcgacgataa cgagttcctc ttccaactgg gactgatccg ggaaacaccg
      961 caagggagac ttcctacaca cgcggcggtt ttactcttcg gttctgacgg ctatctgcga
     1021 ggcatgatgc ctcgtcccgt ggcggattgc cagcgcttcg gtttcgagtt cggcgagaat
     1081 ccgcccggcg tacgctggct ggaccgtgtg gtcatggagt ccaacatcgt tcaggcgtgg
     1141 ctctctttcc tggactggta tatgaaattc gcctccatcc cctttcggct cgacccgacg
     1201 accatggaga gggacgacac tcccgccgat tttattgcct tccgagaggc cttcgtcaat
     1261 gtgctgtgcc atcaggacta ccaggaccac acccgtaagc ccgagatccg gcgcttttcg
     1321 gatcgcacca tcttctggaa tcccggcgac gccttcgcca ccggcgatct gctcgccccc
     1381 ggtccgaagg aggttcgcaa tccgactatc gccgtagcgt ttcgccgcat cggtttcagt
     1441 gaaaatgccg gttggggctt gaacgacgta atttcccaat ggaccggact tggctatgtc
     1501 cgccctgtta tccgcaacgg caaggcaagc aaggatttcg agttgatgtt gatcgcggcg
     1561 agctctcctg tttcttcagg gcgaatccag ccgggagagg gcgtcgagca agtaacccag
     1621 caagtaaccc agcaagtaac ccagcaagta acccagcaag tagaaaaatt gctggcagcc
     1681 tgcacttccg agttgagtag atcggaactg atgaaagctg tccgtatcag agatagagtc
     1741 agcttctccc gtaattatct ggagcccgct cttgcagaaa gtttgatcga aatgacgcag
     1801 cccgactcgc cgaaaagccc tacccagaag taccggctca cagccaaagg cagagtgctc
     1861 ctggagtccg tcggcagttc gaaatgaagt gcgcgagaag ttgtggaaaa ggagaccgat
     1921 tgagaacaag agcaacgc
//
LOCUS       DAOTIL010000547         1938 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminobacteriaceae bacterium isolate AMR_MDS_3026
            NODE_70038_length_1938_cov_2.432820, whole genome shotgun sequence.
ACCESSION   DAOTIL010000547 DAOTIL010000000
VERSION     DAOTIL010000547.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868050
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminobacteriaceae bacterium (wastewater metagenome)
  ORGANISM  Aminobacteriaceae bacterium
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae.
REFERENCE   1  (bases 1 to 1938)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1938)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:19:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,224
            CDSs (total)                      :: 3,198
            Genes (coding)                    :: 3,184
            CDSs (with protein)               :: 3,184
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1938
                     /organism="Aminobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_70038_length_1938_cov_2.432820"
                     /isolate="AMR_MDS_3026"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:3034609"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            244..1212
                     /locus_tag="P5201_10610"
     CDS             244..1212
                     /locus_tag="P5201_10610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014806274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxyacid dehydrogenase"
                     /protein_id="HRV99035.1"
                     /translation="MKVLCWGKAFQRFFDTLSTVFAHHTMSIADPLDDKGALNDAEVL
                     VIRPATVDEELLARAPSLRMVQQWGAGTEGIDVAACSKRGVIACNVPSLGTGNAEGVA
                     EVAFLHMLLHAKRFFRAREKLMEGKVYTPPGTALWGKRGCVVGLGNVGRSVALRMKAF
                     GMNVTGVNRTPPSEEGIVDDFFPLSGLEQAVKGRRFVVVALALNAATENIFGESFFKA
                     MDRDAFFINVARGGLVRRDALENALKEQWIAGAGLDVLWEEPHSPEDPILTHPAVTVT
                     PHIGGVNDASFEGVLSFIVRNIDLLASGKNPLSRLDIKGAGASQSR"
     gene            1217..>1938
                     /locus_tag="P5201_10615"
     CDS             1217..>1938
                     /locus_tag="P5201_10615"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRV99036.1"
                     /translation="MTAYLIKRIFYFAAVLAVATGTIWVYQYWWEQNALESPDVVSAV
                     PYTYIERIPVKGVLIWEETVLVSRWDGNLSYPEPGTRRVARGETVAVIGSSSGKMAVR
                     SESVGYFVPGLDGAEGEWRYSFHWPGMAPLPASPAFAEFSSGVFVRRGDAVGKLISQP
                     QELRCVLYADVTPALQKDIQTGFVRVKTEAHGWPGRAAVRASRFLGTKMKLYLTLPFF
                     SLENVKSREIALFLETGEHVGVA"
BASE COUNT          382 a          457 c          600 g          499 t
ORIGIN      
        1 actgttttat gcagtcggtc gatgtttttt aagcatcttt ctgtggattt ttcgtgtctg
       61 ggagaaaata tgatcctcta cgttctttct cctgcaggct cgttttgcag cagaatggtg
      121 taaaatcaaa cagagtgtat tgctatcatg gaaataaagg gtgattgcgc tctgttcact
      181 gtctttccgt agggactttt ctttgacgtt atgctgaaag attcctgcgt gaggaggaag
      241 aagatgaagg ttctctgttg gggaaaggca tttcaacgtt ttttcgatac gctctccacg
      301 gtgtttgcac atcacacgat gagcatcgcg gatcctctcg acgacaaggg ggccttgaat
      361 gacgcggaag tcctggtgat tcgccctgcc actgtggacg aggagctgct cgcccgcgct
      421 ccttcgctgc gtatggtgca gcagtggggg gcgggtaccg aggggattga tgttgcagcc
      481 tgttcgaagc gcggggttat tgcgtgcaat gtcccttctc ttgggacagg gaacgccgag
      541 ggagtggctg aagttgcatt tctgcacatg ctgctccacg caaaacgttt tttccgcgcc
      601 agagaaaaac tcatggaggg aaaggtctac acgcctccgg ggacggcgct ttggggcaag
      661 cgcggatgcg tggtaggtct tggaaatgtg gggagatccg tagcgcttcg tatgaaggcg
      721 ttcggcatga atgtgacggg cgtgaatcgt acgccccctt cggaagaggg gattgtggat
      781 gatttcttcc ctctttccgg tctggagcag gcggtgaagg ggcgccggtt cgtagtggtt
      841 gcgctcgcgc tgaatgctgc taccgagaat attttcggcg agtctttttt caaggcaatg
      901 gacagagatg ccttcttcat caatgtggca agaggagggc ttgttcgcag agacgcgctt
      961 gaaaacgccc tgaaggagca gtggattgcc ggcgcaggcc tcgatgtgct gtgggaggaa
     1021 ccgcattctc cggaagatcc catccttact caccccgccg tcacggtaac gccccatatc
     1081 ggtggagtga acgacgcatc ctttgaaggc gtgctctcgt ttatcgttcg caatatcgat
     1141 cttcttgctt cggggaaaaa tccgctctcg cgtctcgaca tcaaaggagc gggggcttcc
     1201 cagtcccgtt aggcgtatga ctgcgtacct tatcaagcga atattctatt ttgccgcggt
     1261 gcttgccgtt gcgacgggaa cgatatgggt gtatcagtac tggtgggagc agaacgccct
     1321 tgaaagccct gacgtagtat cggccgtgcc ctatacgtac atcgaaagaa tcccggtcaa
     1381 gggagtcttg atatgggaag aaaccgttct tgtttcacgc tgggacggaa atctttcgta
     1441 tccggagccg ggaacgagaa gagtcgcgag aggcgaaacc gttgcggtta tcggttcatc
     1501 ctcggggaaa atggctgttc gttcagagtc tgtcggatat tttgttcccg gcctggacgg
     1561 ggctgaagga gagtggcggt attcgttcca ctggcccggt atggcgccgc tgccggcgtc
     1621 tccggctttt gccgaatttt catccggggt gtttgtccgc cggggagatg ccgtgggaaa
     1681 gctcatatct cagcctcagg agcttcggtg cgtcctctac gccgatgtga cgcccgcgct
     1741 ccaaaaagat atacaaacag gttttgtgcg tgttaagacc gaggcgcacg ggtggccggg
     1801 aagggctgcc gtccgtgcgt cgcgtttttt gggtacgaaa atgaagctct atcttacatt
     1861 gccgttcttt tctctcgaga atgtcaaatc cagggagatt gcgctgtttc tcgagacggg
     1921 ggagcatgtc ggtgttgc
//
LOCUS       DAOTIL010000548         1923 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminobacteriaceae bacterium isolate AMR_MDS_3026
            NODE_70812_length_1923_cov_2.911670, whole genome shotgun sequence.
ACCESSION   DAOTIL010000548 DAOTIL010000000
VERSION     DAOTIL010000548.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868050
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminobacteriaceae bacterium (wastewater metagenome)
  ORGANISM  Aminobacteriaceae bacterium
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae.
REFERENCE   1  (bases 1 to 1923)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1923)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:19:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,224
            CDSs (total)                      :: 3,198
            Genes (coding)                    :: 3,184
            CDSs (with protein)               :: 3,184
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1923
                     /organism="Aminobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_70812_length_1923_cov_2.911670"
                     /isolate="AMR_MDS_3026"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:3034609"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            182..493
                     /locus_tag="P5201_10620"
     CDS             182..493
                     /locus_tag="P5201_10620"
                     /inference="COORDINATES: protein motif:HMM:NF037100.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase domain-containing
                     protein"
                     /protein_id="HRV99037.1"
                     /translation="MLSQKSLEFIKEIGEKHGVQRIVLFGSCLHLPETEAGDIDLAVG
                     GLDAETFVEMWNDLTWASELHMKNVDLVRMEDALPVMVMVRAEGVTIYECERERATVS
                     A"
     gene            456..899
                     /locus_tag="P5201_10625"
     CDS             456..899
                     /locus_tag="P5201_10625"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRV99038.1"
                     /translation="MNAKESARQFLREVWRLIKEDTVPSMQEYLNLPEWEKIQLAGMG
                     MCLGDIYKAFEQSLRCILENIEGKKIEKNESWHSNLLRLSNEMELIPTDIRDTVQGML
                     KYRHHLHHGYGVKLNERLIRKYAPEAIDAFDAFTKHIISRYFDIL"
     gene            complement(956..1711)
                     /locus_tag="P5201_10630"
     CDS             complement(956..1711)
                     /locus_tag="P5201_10630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014163570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HRV99039.1"
                     /translation="MELVWYHWAGIVLVGVMQGFLNTVAGGGTLLVLPALTFLGMDLA
                     VANGTNRIAILLQSISGAASFKQQKALSFKVALPLAAAATLGALVGTFVVVQVDRRSL
                     NLVIAALITLMALLLVFKPKMWEEVKEQHWPKWGVYLAFFAVGIYGGFIQAGVGFFLS
                     WALVVAAGMDLVSGNAVKTIIIGCYTTISLAIFYMNGLVNVPVGLVLAIGSMAGAVLG
                     ARFTVMKGNKWVRYVLAFVVVISAVKMVMDAIG"
BASE COUNT          476 a          588 c          504 g          355 t
ORIGIN      
        1 gacgccctca cgctgaacta cctgatgaag cacaaggccg tcaacagcac cgccgacgtc
       61 ctgacgaagg cgggcctctc cttcgtcgcg gcgggcgcgc accggctgta gagatggtag
      121 aataagaacg aaaaggggac gcgaaaaact cattcagcgt cgcctgcaag gagggagagt
      181 tgtgctctcg caaaaaagcc ttgaattcat caaagaaata ggagagaaac acggagtgca
      241 acgcattgtg ctcttcggtt cctgcctgca tcttccggag acggaagcgg gcgacatcga
      301 cctggccgtc ggcggactcg acgcggaaac gtttgtcgag atgtggaacg acctcacgtg
      361 ggcttctgaa cttcatatga aaaacgtcga cctcgtacgg atggaggatg ccctgcccgt
      421 catggtgatg gtccgcgccg aaggagtgac gatctatgaa tgcgaaagag agcgcgcgac
      481 agtttctgcg tgaagtgtgg agacttataa aagaagacac cgtgccgtcg atgcaggagt
      541 atctcaacct gccggaatgg gaaaaaatcc agcttgccgg catgggcatg tgccttggag
      601 atatttataa agccttcgaa cagtccttgc ggtgtattct cgaaaatatc gaaggcaaaa
      661 agatcgagaa gaacgaatcc tggcacagta acttgttgcg cctttccaac gaaatggagc
      721 tcatccccac agatattcgg gacaccgtac agggtatgct taagtacagg catcaccttc
      781 accacggcta cggcgtcaag ctgaacgagc ggcttatacg aaaatacgcg cccgaggcaa
      841 tcgatgcctt cgatgccttt acaaaacata ttatcagccg ctactttgat atcctttgaa
      901 tcgaacaaag ccccgggcag tgacaccggg gcttcatcat ttctattggt tgcttctacc
      961 ctatcgcgtc catcaccatc ttcaccgcgc tgatgaccac cacgaaagcc agcacgtagc
     1021 ggacccactt gtttcccttc atcaccgtga agcgcgcgcc gagcaccgcg cccgccatgc
     1081 tcccgatcgc gagcaccagg cccacaggca cgttcaccag gccgttcatg tagaagatcg
     1141 ccaggctgat cgtcgtgtag cagccgatga tgatcgtctt caccgcgttg ccgctcacca
     1201 ggtccatccc cgccgccacc acgagcgccc acgaaaggaa gaagccgacg cccgcctgga
     1261 tgaagccgcc atagataccc accgcgaaga acgcaaggta gacgccccac ttcggccagt
     1321 gctgctcctt cacctcttcc cacatcttcg gcttgaagac aagcagcaac gccatcagcg
     1381 tgatcaaggc cgcgatgacg aggttcagac tcctcctgtc cacctgtacc acaacaaacg
     1441 tcccaacgag cgcgcccagc gtcgccgcag cggccagcgg cagcgccacc ttgaacgaga
     1501 gcgccttctg ctgcttgaag ctcgccgccc cggagatgct ctgcaacagg atggcgatgc
     1561 ggttcgtccc gttcgccacc gccaggtcca tccccaggaa ggtgagcgcc ggaagcacaa
     1621 gcagcgttcc gccgcccgcg accgtattca aaaagccctg catcacgccg acgaggacga
     1681 tccccgccca gtggtaccat accagttcca tacctctcac aaccttcttc aaatgataga
     1741 gggagcctct tccgaggctc ccgtacgctc ggcatgctca caacatgttt tttgcagtat
     1801 atcaaatcgg agaacggatg aaaagaggcg ttttttctac gaggcttaca cccttgctca
     1861 acgaagccgg agcgctccta taaacgccgc aagagaagca ccatgagcgg gagcagcaag
     1921 agc
//
LOCUS       DAOTIL010000549         1923 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminobacteriaceae bacterium isolate AMR_MDS_3026
            NODE_70829_length_1923_cov_2.387045, whole genome shotgun sequence.
ACCESSION   DAOTIL010000549 DAOTIL010000000
VERSION     DAOTIL010000549.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868050
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminobacteriaceae bacterium (wastewater metagenome)
  ORGANISM  Aminobacteriaceae bacterium
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae.
REFERENCE   1  (bases 1 to 1923)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1923)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:19:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,224
            CDSs (total)                      :: 3,198
            Genes (coding)                    :: 3,184
            CDSs (with protein)               :: 3,184
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1923
                     /organism="Aminobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_70829_length_1923_cov_2.387045"
                     /isolate="AMR_MDS_3026"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:3034609"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1923)
                     /locus_tag="P5201_10635"
     CDS             complement(<1..>1923)
                     /locus_tag="P5201_10635"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRV99040.1"
                     /translation="LEIYRPISHLKTGRFTGSLHPSAAAKGGSHAIVLLHHTSLSSGG
                     TFARLLRLEGDTLKEVSAARLSGFSNPDPAHLLVGDLDRDSMVLGYPTHITLENHVTP
                     LLFMNEPPKHIDADGTGAVTNSTRRDDLYVQYAQSDSESRETTNTFEAESTLGGSVSV
                     EQKGGIDLGIVKAEAKLTTTVGGSTKDLRSEIARSYQSRSTSLTGATTRDDVLLYLSN
                     RVHLWRYPILGWMSEPEGGGAKVPTYYQLSIPEAEASMKFLPGLNVDWYHPAHMNGNV
                     LSYPSRRAQIGDFAEDRRLSDNVEFGVGGGTAFTRGITWKDSFTRESLKSVEVKMNLD
                     VELQVSGSAQVEIFEVGGSTTASFHMDKRTTETSVAKTSVEKEKAFTVNIPTFSTPLG
                     YQITPLMYRTGGGVLKTSHVVDVLNNRSLWSSRYAAPDPALNLPRLWRYVEGDGWKWA
                     AADPLNPDARKIRGILFRNANGVDLGKSLPLGETTTIAVRVHNFSLAECPEVDVLFEA
                     VPYGGGPAVVIGTARTPRIDPWGASEAANWKWATVAWNTSGMTEGNYTVRVTANPDGR
                     VAELEGRALNEPFDNNLGWYDVFVGRSRSAVEEELAAVNENLVAATLELANTPADGKA
                     FALGETALLRGMLRNE"
BASE COUNT          288 a          608 c          626 g          401 t
ORIGIN      
        1 cttcgttgcg cagcattccg cgaagcagcg cggtttcgcc gagagcgaag gccttgccgt
       61 cggcaggggt gttcgccagt tcgagcgtcg cggcgacgag gttctcgttc acagcggcaa
      121 gttcctcttc gacggcgctc cgcgacctgc ccacgaagac gtcgtaccag ccgaggttgt
      181 tgtcgaacgg ctcgttcagg gcccgtccct caagctccgc aacccgcccg tcggggttcg
      241 ccgtgacgcg taccgtgtag ttcccctccg tcatccctga ggtgttccac gcaactgtcg
      301 cccacttcca gttggccgct tcggaagcgc cccacgggtc tatccggggc gtccgtgcgg
      361 ttccgataac gaccgccggg ccgccgccgt agggaacggc ctcgaagagg acgtccacct
      421 cggggcactc ggccaggctg aagttgtgca cgcgcacggc gatcgtcgtt gtttctccca
      481 gcgggaggct ctttcccagg tcgacgccgt tcgcgttccg gaagaggatg ccgcgtatct
      541 tcctcgcgtc cgggttgagc gggtcggcgg ccgcccactt ccatccgtcg ccttcgacgt
      601 acctccagag gcgcggaagg ttgagggccg gatcgggggc cgcgtagcgg ctcgaccaca
      661 gcgaccggtt gttgagcacg tccacgacgt gggatgtttt gaggacgccg ccgcccgttc
      721 tgtacatcag cggcgtgatc tgatagccga gcggcgtcga gaacgtcggg atgttcacag
      781 tgaacgcctt ttccttctcc acggaggtct tcgccacgct cgtctcggtc gtccgcttgt
      841 ccatgtggaa ggaggcggtc gtcgagccgc cgacctcgaa gatctcgacc tgcgcgctgc
      901 cgctcacctg cagttccacg tcgaggttca ttttgacctc gaccgacttc aggctctccc
      961 tcgtaaacga gtccttccac gtgatgcctc ttgtgaacgc cgttccgccg ccgacgccga
     1021 actcgacgtt gtcggagagt cgcctgtctt cggcgaaatc gcctatctgt gctcttctcg
     1081 aagggtagga gaggacgttt ccgttcatgt gggccggatg gtaccagtcg acgttcagcc
     1141 cgggaaggaa tttcatgctc gcttccgctt ccgggatgga gagctgatag taggtgggta
     1201 ctttcgcccc gcctccttcc ggttcgctca tccagccgag gatcgggtag cgccagaggt
     1261 ggacgcggtt ggagagatag agcagcacgt cgtccctcgt ggtcgccccg gtgagcgacg
     1321 tggatctcga ctggtagctc ctggcgatct cggagcgcag gtctttcgtc gacccgccga
     1381 cggtggtggt cagcttcgcc tcggctttca ctatgccgag atcgatccct cccttttgct
     1441 ccacgctcac cgagcctccg agggtgctct ccgcctcgaa ggtgttggtg gtctctctgg
     1501 actccgagtc gctttgcgcg tactggacgt acaggtcgtc cctgcgcgtg ctgttcgtca
     1561 ccgctccggt tccgtccgcg tcgatgtgct tcggcggctc gttcatgaag aggagcggcg
     1621 tgacgtggtt ctccagcgtg atgtgcgtgg ggtagcccag caccatgctg tcgcggtcga
     1681 ggtcgccgac gaggagatgg gcggggtcag gattcgagaa gccgctgaga cgagctgcgg
     1741 aaacttcttt caacgtatcg ccctcaagac gaagaagacg ggcgaacgtc ccgccggagg
     1801 acaaactggt gtgatggagc aggacaatcg cgtgcgatcc gcctttcgcc gccgccgacg
     1861 ggtggagcga gccggtgaag cgccccgtct tcagatggga gatgggccgg tagatctcga
     1921 gtc
//
LOCUS       DAOTIL010000550         1921 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminobacteriaceae bacterium isolate AMR_MDS_3026
            NODE_70921_length_1921_cov_1.904073, whole genome shotgun sequence.
ACCESSION   DAOTIL010000550 DAOTIL010000000
VERSION     DAOTIL010000550.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868050
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminobacteriaceae bacterium (wastewater metagenome)
  ORGANISM  Aminobacteriaceae bacterium
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae.
REFERENCE   1  (bases 1 to 1921)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1921)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:19:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,224
            CDSs (total)                      :: 3,198
            Genes (coding)                    :: 3,184
            CDSs (with protein)               :: 3,184
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1921
                     /organism="Aminobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_70921_length_1921_cov_1.904073"
                     /isolate="AMR_MDS_3026"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:3034609"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1570)
                     /locus_tag="P5201_10640"
     CDS             complement(<1..1570)
                     /locus_tag="P5201_10640"
                     /inference="COORDINATES: protein motif:HMM:NF037510.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
                     /protein_id="HRV99041.1"
                     /translation="MNEAETRAEYIDPALKTAGWGEAGGSRVRREYVISPGRIEGRGR
                     RGKSLTADYVLEFRNAKLAVVEAKSRDEALTAGAAQAKNYAEKLQVRFAYATNGQGIY
                     EIDMMTGREREIPFYPSPEDLWRRIHAVQNEWRDRFFEVPFEDKSGSHPGRYYQDIAV
                     ERVLHAVAEGKKRILLTLATGTGKTFIAFQVAWKLFHSRWNLSREPARRPRILFLADR
                     NILADQAYNAFSSFPEDAMVRISPADISKKGRVPKNGSLFFTIFQTFMTSPRRDEESS
                     FCFGEYAPDFFDFIIVDECHRGGANDESTWRGILEYFAPAVQLGLTATPKRAENIDTY
                     AYFGEPVFVYSLKEGINDGFLTPFRVKQIATTLDEYFYTPDDTLVEGEVEQGKIYTES
                     DFNRIIEIKERERKRVRIFMEQIDQREKTLVFCANQEHALFVRDLVNQTKTSADPNYC
                     HRVTADDGELGEQRLRDFQDNEKTIPTVLTTSQKLSTGVDARNIRNIVLLRPIRQIIE
                     FKQIIGRGTRLYEEK"
     gene            complement(1609..>1921)
                     /locus_tag="P5201_10645"
     CDS             complement(1609..>1921)
                     /locus_tag="P5201_10645"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRV99042.1"
                     /translation="ALHRVRFHKPEHNKWFVYFLFACFQNGTLREYFSGTGIQHFTGE
                     ALSRFVVPVPPTQQIQAEVAKFDALAVETQRLASIYHRKLAALDELKKSLLHQAFTGQ
                     L"
BASE COUNT          366 a          546 c          546 g          463 t
ORIGIN      
        1 ctttctcctc gtacagccgc gtgcctctgc ctatgatctg cttgaactcg atgatctgcc
       61 tgatcggacg cagaaggacg atgtttcgga tattccgggc gtcaactcct gtggagagct
      121 tctgcgaggt ggtcagaaca gtcgggatgg tcttctcgtt gtcctgaaag tcccgcaggc
      181 gctgttcgcc gagctcgccg tcgtccgcag tcactcgatg gcagtagttc gggtccgcgc
      241 tcgtttttgt ctggttgacg agatcgcgga caaagagcgc gtgttcctga ttcgcgcaga
      301 aaacaagcgt cttctctctc tggtcgatct gctccatgaa aatgcgcacg cgctttcttt
      361 cgcgctcctt aatctcgata atcctgttga agtcgctctc ggtgtagatt ttcccctgct
      421 ccacctcccc ttcgacgagc gtatcgtcgg gggtgtagaa gtactcgtcg agcgtggtcg
      481 caatctgctt cactctgaaa ggcgtcaaaa aaccgtcgtt gattccctcc ttcagagagt
      541 acacaaagac gggctctccg aagtaggcgt aggtgtcgat gttctcggcc cgcttcggcg
      601 tcgcggtgag tccgagctgc accgccggtg caaagtattc cagaataccg cgccacgtgc
      661 tctcgtcgtt ggctccgcct ctgtggcact cgtcgacgat gatgaaatcg aagaaatcgg
      721 gagcgtactc gccgaaacag aaggaggact cttcatccct tcgagggctt gtcatgaaag
      781 tctggaagat ggtgaagaaa agactgccgt tcttcggcac gcgtcctttt tttgaaatgt
      841 ccgcagggga gatccgaacc atcgcatcct ccgggaacga ggagaatgcg ttgtacgcct
      901 ggtcggcgag gatgtttctg tccgcgagga agaggatgcg ggggcgtctc gcaggctcgc
      961 gggaaagatt ccatcgactg tgaaagagct tccatgcaac ctgaaaggcg atgaaggtct
     1021 ttcccgtacc ggtggcgagc gtcagcaaaa tgcgcttttt cccctccgct accgcgtgca
     1081 gcacccgctc gaccgcgatg tcctggtagt accgtccggg gtgggaaccg ctcttatcct
     1141 cgaaagggac ctcgaaaaaa cggtcgcgcc actcgttctg cacggcgtgg attcgccgcc
     1201 atagatcttc cggggagggg tagaaaggaa tctcccgttc cctgcccgtc atcatgtcga
     1261 tctcgtaaat cccctgcccg ttcgtagcat aggcaaaacg cacttgcagt ttttcggcgt
     1321 agttcttcgc ttgggcggcg cccgcggtga gggcctcgtc ccgcgacttc gcctcgacca
     1381 cggccagctt cgcgttccgg aactcgagca cgtagtcggc ggtcagcgac ttgcctcgcc
     1441 ttccgcgccc ttcgatacgc cccggcgaga tgacatactc ccgtctgacg cggctgcctc
     1501 cggcttcacc ccatcccgcc gttttcagcg ctgggtcgat gtattcggct ctggtttcgg
     1561 cttcgttcat agaggatcac ctctccgcgg ttcaaacggc ttgcgtcttc agagttgccc
     1621 ggtgaacgcc tggtgcaaca gcgatttttt cagttcgtcc agcgcggcga gcttgcgatg
     1681 gtaaatggag gcaaggcgct gggtttctac ggcgagcgcg tcgaattttg cgacttcagc
     1741 ttggatttgt tgcgtaggag gcacgggaac aacaaatctg gagagtgctt ctccggtaaa
     1801 atgttgtatg ccagtaccgc tgaaatactc tcgcagggtt ccgttttgaa aacatgcaaa
     1861 aagaaaatac acaaaccatt tattgtgctc tggtttgtga aaacgtactc tatgcagcgc
     1921 t
//
LOCUS       DAOTIL010000551         1919 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminobacteriaceae bacterium isolate AMR_MDS_3026
            NODE_70982_length_1919_cov_4.053112, whole genome shotgun sequence.
ACCESSION   DAOTIL010000551 DAOTIL010000000
VERSION     DAOTIL010000551.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868050
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminobacteriaceae bacterium (wastewater metagenome)
  ORGANISM  Aminobacteriaceae bacterium
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae.
REFERENCE   1  (bases 1 to 1919)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1919)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:19:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,224
            CDSs (total)                      :: 3,198
            Genes (coding)                    :: 3,184
            CDSs (with protein)               :: 3,184
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1919
                     /organism="Aminobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_70982_length_1919_cov_4.053112"
                     /isolate="AMR_MDS_3026"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:3034609"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..498)
                     /locus_tag="P5201_10650"
     CDS             complement(<1..498)
                     /locus_tag="P5201_10650"
                     /inference="COORDINATES: protein motif:HMM:NF013793.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HRV99043.1"
                     /translation="MSPREIVVVSGKGGTGKTTLTACFAALAEGPRIMADADVDAPNL
                     ELLLSPRETEKRPFFGMEVAHVDAARCTGCGECERFCRFRAISVRDGKAEVDSGFCEG
                     CGGCLLVCPEACISKEKFQRGVLRRGETDFGSMWYARLTPGGENSGMLVALLRKEAKR
                     EAVERG"
     gene            complement(495..1337)
                     /locus_tag="P5201_10655"
     CDS             complement(495..1337)
                     /locus_tag="P5201_10655"
                     /inference="COORDINATES: protein motif:HMM:NF013793.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HRV99044.1"
                     /translation="MKIAVASGKGGAGKTSIAASLVLAKRGVLAMDLDVEEPNLGILL
                     GCSPGESRPVHQPVAYVEPDLCSGCGRCAQACAYGAIAWFGSGLPVVNESLCHGCGVC
                     PLVCPTGAMREREKVIGEIRSGFVDGAPFLEGRLEVGGVSTVRLIEATIQAGESLHVD
                     DWIMDAPPGASCPVSAVLRAADAAILVVEPTPFGLSDVAGVLEVVADMKKPAGIVANK
                     TGMGDLSLDDLCRRFSVEVLARYPFTRSAAEAGARGVCPYRSEETWVWKTESLWRAIE
                     RRFS"
     gene            complement(1334..1696)
                     /locus_tag="P5201_10660"
     CDS             complement(1334..1696)
                     /locus_tag="P5201_10660"
                     /inference="COORDINATES: protein motif:HMM:NF014620.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NifB/NifX family molybdenum-iron cluster-binding
                     protein"
                     /protein_id="HRV99045.1"
                     /translation="MTKAAIAMDSGVISEHFGKTKEFLFITFEGKNEISREILPPPAE
                     EHVPGLFPNWVKKMGADVVVAGGMGTQAKRMFESLGVGVCTVPSMGVEQAVEALLSGS
                     LQGVDMECTHGHDHSCGK"
     gene            complement(1693..>1919)
                     /locus_tag="P5201_10665"
     CDS             complement(1693..>1919)
                     /locus_tag="P5201_10665"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRV99046.1"
                     /translation="GPCGRGVAAGGRFFRGRGCFGWGMRMGMGMGRGGVWAEPYTGAD
                     EQTLLEQELEMTEARAQELRARLGKGGDAE"
BASE COUNT          358 a          710 c          531 g          320 t
ORIGIN      
        1 gcctctttca acggcttcac gcttcgcctc cttgcgcaga agggcgacca gcataccgga
       61 gttctcgcca ccgggcgtca gacgagcata ccacatcgaa ccaaaatccg tctcgccgcg
      121 gcggagcact cctcgctgga acttctcctt ggagatgcac gcctcggggc agacaagcag
      181 gcatcctccg cacccttcgc agaaaccgga gtccacctcc gcctttccgt cccggacgga
      241 gatcgccctg aagcggcaga agcgctcaca ctccccgcat cccgtgcagc gagcggcgtc
      301 cacatgcgcg acctccatac cgaagaaggg gcgcttttcc gtctctcggg gggaaagaag
      361 cagctccagg ttcggcgcgt ccacgtcggc gtcggccatg atcctcggcc cctccgcaag
      421 cgctgcaaaa caggccgtga gggtcgtctt ccctgtgccg cccttgccgc tgacgacaac
      481 gatctcgcgc ggactcatga gaatctcctt tcaatggcac gccagaggga ctctgtcttc
      541 catacccacg tttcctcgga gcggtacggg cagactcccc tggccccggc ctccgcggcg
      601 gagcgggtga aggggtagcg cgcgagcacc tcgacggaga agcggcggca caggtcatcc
      661 agcgatagat cgcccatgcc ggtcttgttc gcaacaattc ccgcaggttt tttcatgtcc
      721 gcaaccacct cgagcacacc cgcgacatcc gaaagaccga acggcgtcgg ctcgacgaca
      781 aggatggcgg cgtccgctgc gcgcagcacc gccgacacgg gacaagaagc gccaggaggc
      841 gcatccatga tccagtcgtc gacgtgcagc gactcgcccg cctggatcgt cgcctcgata
      901 agccgtaccg tgctcacgcc tccgacctca agccgtcctt cgaggaaggg cgccccgtcg
      961 acgaatccgg agcggatctc gccgatcacc ttctcgcgct cacgcatcgc ccccgtcggg
     1021 cagacgagcg gacagacgcc gcacccgtga cagagcgatt cgttcaccac gggaaggccg
     1081 gagccgaacc atgcgatggc gccgtacgcg cacgcctgtg cgcatcgtcc gcagcccgag
     1141 cagagatcgg gctccacata cgccacaggt tgatgcaccg ggcgcgattc accgggcgaa
     1201 caccccagaa gaatccccag attgggttcc tccacgtcga gatccatcgc gagcacccct
     1261 cgctttgcaa gaacaaggga ggccgcgatg ctggtcttgc ccgcgccccc cttgccgctc
     1321 gccacggcga tcttcatttt ccgcaggagt ggtcgtgtcc gtgggtgcac tccatatcga
     1381 cgccctgcag agaaccggag aggagcgcct ccacagcctg ctccacaccc atggagggaa
     1441 ccgtgcagac accgacgccc agcgactcga acatccgctt cgcctgcgtt cccatgcctc
     1501 ccgcgaccac cacgtcggcc cccatcttct tcacccagtt cgggaagagc ccgggaacat
     1561 gctcttcggc gggaggagga aggatctcgc gcgagatctc gttcttcccc tcgaaggtga
     1621 tgaaaagaaa ctccttcgtc ttgccgaagt gctcggaaat aacgccgctg tccattgcaa
     1681 tcgccgcttt cgtcactctg cgtccccacc ctttcccagt ctggcgcgca gctcctgcgc
     1741 ccttgcctcg gtcatttcaa gctcctgttc gagcagtgtc tgttcgtcgg ccccggtata
     1801 cggctcggcc cacacgccgc ctctgcccat acccattccc atacgcatgc cccaaccaaa
     1861 gcagccgcgc cctctaaaaa accttccgcc tgctgcgacg ccccgcccgc aagggccgc
//
LOCUS       DAOTIL010000552         1918 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminobacteriaceae bacterium isolate AMR_MDS_3026
            NODE_71048_length_1918_cov_3.474503, whole genome shotgun sequence.
ACCESSION   DAOTIL010000552 DAOTIL010000000
VERSION     DAOTIL010000552.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868050
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminobacteriaceae bacterium (wastewater metagenome)
  ORGANISM  Aminobacteriaceae bacterium
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae.
REFERENCE   1  (bases 1 to 1918)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1918)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:19:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,224
            CDSs (total)                      :: 3,198
            Genes (coding)                    :: 3,184
            CDSs (with protein)               :: 3,184
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1918
                     /organism="Aminobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_71048_length_1918_cov_3.474503"
                     /isolate="AMR_MDS_3026"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:3034609"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            42..521
                     /locus_tag="P5201_10670"
     CDS             42..521
                     /locus_tag="P5201_10670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013047964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CtsR family transcriptional regulator"
                     /protein_id="HRV99047.1"
                     /translation="MASLTRVIENYINNLFSEMDEQTVSLRRKELAEIFGCVPSQINY
                     VLRSRFTPERGFIVESQRGGHGYIRIVRICYDIPEEKVNHIDEIVGDSITEQEARRIL
                     TSLQERGMIDSRERLLIEVALRHTDELGSTEFDISPYKRGALAAELLKRLLRSLALA"
     gene            570..>1918
                     /locus_tag="P5201_10675"
     CDS             570..>1918
                     /locus_tag="P5201_10675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005661584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Clp protease N-terminal domain-containing
                     protein"
                     /protein_id="HRV99048.1"
                     /translation="MWHFFTERGRKVVQLAHREALRLGHDVVGTEHVLLAILSEGEGA
                     GVQTLEALGVNLAELRSRIESTVGKSHPILKPVDLPLSPRAKRVFDLSVREARNMGVN
                     YVGTEHILLGILAEGESAAARVLLGLGIDHATLAKEIMRFLEGENGSGKGTENSADNR
                     RKNGRSKTPTLDQLALDLSERSRNGELDPVIGRSKEIRRIVQILSRRTKNNPVLIGEP
                     GVGKTAIVEGLAQKIMEGDIPELLKDKKVMQLNMGNLVAGTKYRGEFEERMRKLVKEL
                     SEGRDVILFIDEIHTIVGAGGAEGAVDAANILKPSLSRGEFQVIGATTLEEYRKHIEK
                     DAALERRFQPVFVQEPSVDDSIRILEGLRDRYESHHKARIQDDALEAAARLSARYIMD
                     RRLPDKGIDLIDEAAARARIKTMEPPEELKTLEQRLEEIRKEKEAAVDGQEFERAAEL
                     "
BASE COUNT          497 a          434 c          573 g          414 t
ORIGIN      
        1 tatgttttgg tgtatggaaa ttgcagtttg gagggaatgg aatggcaagt ttgacacggg
       61 tcatcgaaaa ctacataaat aaccttttct ccgaaatgga tgagcagaca gtctcgcttc
      121 gtagaaagga gctcgcggaa attttcggat gtgttccgag ccagatcaat tatgttctcc
      181 gaagccgctt tacgcccgag agaggattta tcgttgagag tcagagaggc ggtcacgggt
      241 atattcgaat agtccgtata tgctacgata taccggaaga gaaagtaaat catattgatg
      301 aaatagtagg agattccatc accgagcagg aagcccggcg tatccttacg tctcttcagg
      361 agcgcgggat gattgattcg agagaacgtc ttctcattga ggtggcgttg cgacacactg
      421 acgaactggg tagcaccgag tttgatatct ctccctataa gagaggcgcc ctggcggcgg
      481 aactgttgaa gcgtttgctt cgcagtctcg ctctggcctg atggcaggat gagaacggcc
      541 cttcggggcc atgtaaaggg tggtttggaa tgtggcattt tttcacggaa cgcggcagga
      601 aagtggtgca actagcgcac agagaggctc tccgtcttgg acatgacgtt gtgggaacag
      661 aacacgtctt gcttgctatt ctctccgaag gggaaggggc gggtgttcaa acccttgaag
      721 ctcttggagt gaatcttgcc gagctgcgct ctcgtattga aagtactgta ggcaaatctc
      781 atccgatttt aaaacctgtg gatttgcctt tgagcccgcg ggcaaagagg gtttttgatc
      841 tttccgttcg cgaagcgcgg aatatgggcg tgaactatgt cggcacggag catatacttc
      901 tcggtatcct cgctgaagga gagagcgccg ccgcccgtgt ccttctgggg ctcggcatag
      961 atcacgctac tctggcaaaa gagatcatgc gatttctcga aggggaaaac ggttcgggta
     1021 aagggacgga aaactctgcc gacaaccgtc ggaagaacgg acgttcgaag acgcctacac
     1081 tggaccagct cgcgttggat ctcagtgagc ggagcagaaa cggtgaactc gatccggtta
     1141 ttggaagaag caaagaaatt cgtagaatcg tccagatcct ctcccgaaga acaaagaaca
     1201 atccggtgct tatcggagaa cccggggtag gaaagaccgc aattgtcgag ggactggcgc
     1261 agaaaattat ggagggagat atccccgagc ttctgaagga taagaaggtt atgcagctca
     1321 acatggggaa tcttgttgca ggcacgaaat acaggggaga gttcgaggag cgcatgcgta
     1381 aactggtgaa ggaactctcc gaaggacgcg acgtcattct cttcatcgac gaaatccaca
     1441 ctattgtcgg cgccgggggc gcagaaggcg cggtggatgc ggccaacatt ttgaagccga
     1501 gtctttccag gggggaattc caggttatcg gcgccactac cctggaagag tacaggaagc
     1561 atatcgagaa ggacgctgcg ctcgaaagac gcttccagcc ggtatttgtt caggagccgt
     1621 ccgtggatga ctccatccgt attctggagg gactcagaga ccggtatgaa tcgcaccaca
     1681 aggccagaat acaggatgac gccctggaag cggccgcccg gctttctgct cgctatatta
     1741 tggacagacg tcttcccgat aaaggaatcg atcttatcga cgaagccgcc gccagagcga
     1801 ggatcaagac gatggagccc cccgaagaac taaagacgct cgaacagcgt ctggaagaaa
     1861 ttcgtaaaga gaaggaagcc gccgtggacg ggcaggaatt tgaaagggct gcggaact
//
LOCUS       DAOTIL010000553         1914 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminobacteriaceae bacterium isolate AMR_MDS_3026
            NODE_71282_length_1914_cov_2.811727, whole genome shotgun sequence.
ACCESSION   DAOTIL010000553 DAOTIL010000000
VERSION     DAOTIL010000553.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868050
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminobacteriaceae bacterium (wastewater metagenome)
  ORGANISM  Aminobacteriaceae bacterium
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae.
REFERENCE   1  (bases 1 to 1914)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1914)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:19:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,224
            CDSs (total)                      :: 3,198
            Genes (coding)                    :: 3,184
            CDSs (with protein)               :: 3,184
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1914
                     /organism="Aminobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_71282_length_1914_cov_2.811727"
                     /isolate="AMR_MDS_3026"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:3034609"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..795)
                     /locus_tag="P5201_10680"
     CDS             complement(<1..795)
                     /locus_tag="P5201_10680"
                     /inference="COORDINATES: protein motif:HMM:NF012707.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HRV99049.1"
                     /translation="MTQPTTYRTPAFFRRIPMLLAASLFLAALLFSSPLCGESLTVKV
                     ALHSGKPGIFIDESGTPAGIFVDILEHIAGIEGWKLEYVPGTWAEGLERLKQGEVDIV
                     TSIAFTPEREKIYTFTGTPALSAWSAAYVARGSGIVSLLDLKGKRVAVMKNTVQESAF
                     IKHIQGFDLDIEILSVPDQDAAFSMVADGTADAAIEVSTTGEVYRQRYELENSAIVFN
                     AVQAYYAAAREDPKDLASAINRHLYAMKADPSSPYYEIMKRWFPKKT"
     gene            complement(838..>1914)
                     /locus_tag="P5201_10685"
     CDS             complement(838..>1914)
                     /locus_tag="P5201_10685"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HRV99050.1"
                     /translation="KLRQILFNLLGNAAKFTKTGGIAVRARADKEHDAEDTVRFLVEI
                     EDTGPGIPEKDLEHIFDAFRQSEAGRDAGGTGLGLSISKRLAELMGGSLTVQSKIGTG
                     TCFTLSVPLHTATSGHRTAKMDRQDIVGLDTGDAFFRILIVDDVKENRDVLRAMLSPD
                     RNAGSATMFRGRTSAFELREAVNGEEALSIFEEWFPHVVLMDMRMPVMDGYEATRRIK
                     SVPGRGSVPVIAITASAFEDDRDEVLKAGVDRYIRKPLRPEELFETLGDVLGLPVLRG
                     GDSAKASGSKGTLPTEEEMKALPGELLAAMHEAVENGDIAGLDELIVKTREIDAGIAE
                     KLRTLARRYDYETLSRLLKNGERG"
BASE COUNT          318 a          595 c          580 g          421 t
ORIGIN      
        1 ggtctttttc gggaaccagc gcttcatgat ctcgtagtag ggcgaggagg gatccgcttt
       61 catcgcgtac agatgccggt tgatggccga tgcaaggtct ttcgggtctt cccgggcggc
      121 ggcgtaatag gcctgtaccg cgttgaagac gatggcgctg ttctccagtt cgtagcgctg
      181 tctgtagact tcgcccgtcg tgctgacctc tattgcggcg tcggcggttc cgtcggcgac
      241 catggagaac gccgcgtcct gatccggaac ggagaggatc tcgatatcga ggtcgaagcc
      301 ctggatgtgc ttgatgaagg ccgactcctg cacagtgttc ttcatcaccg cgacgcgctt
      361 ccccttcagg tcgagcaggg agacgatgcc gctccctctg gcgacgtacg ccgcggacca
      421 tgcggagagc gccggagtcc ctgtgaacgt gtagatcttc tcccgttcgg gggtgaatgc
      481 gatgctcgtg acgatatcga cttccccctg cttcagacgc tccagcccct ccgcccatgt
      541 gccggggacg tactccagct tccagccttc gattccggcg atatgttcga gaatgtcgac
      601 gaagatgccg gcaggggttc cggattcgtc gatgaagatg ccgggtttgc ccgaatgcag
      661 ggcgactttg accgtaaggc tctctccgca aaggggggaa gaaaagagaa gcgctgccaa
      721 aaacagcgag gcggcaagca gcatcggtat gcggcggaag aacgccggag ttcggtatgt
      781 cgtcggctgg gtcatcgttt tcatctcctt cattttcggg aggccggcat gggatgctca
      841 gcctctctcc ccgttcttca gcaggcggga gagcgtttca taatcatacc gcctggcgag
      901 cgtcctgagt ttttcggcga tcccggcgtc gatctccctc gtttttacga taagctcgtc
      961 gagacccgcg atgtctccgt tctcgaccgc ctcgtgcatc gcggcaagca gttctccggg
     1021 aagggccttc atctcctctt cggtcggaag cgttcccttg cttccggacg cctttgcgga
     1081 atcgcccccg cggagaaccg gcaggccgag aacgtctccc agcgtctcga acagctcctc
     1141 cggcctgagc ggttttcgta tgtatctgtc cacccccgct ttcagcactt cgtccctgtc
     1201 gtcttcgaag gcgctcgccg tgatcgcgat gacggggacc gagccccttc ccggaaccga
     1261 cttgatccgc cgcgtggctt cgtagccgtc catgaccggc atgcgcatat ccatcagcac
     1321 gacgtgaggg aaccactctt cgaagatcga cagcgcctct tcaccgttga ccgcttcccg
     1381 cagttcgaag gcggaagtcc tcccccggaa catcgtcgcg gaaccggcgt tccggtcggg
     1441 agagagcatc gcgcgcagca cgtcgcggtt ctccttgacg tcgtcgacga tcagtattct
     1501 gaagaaggcg tctcccgtgt ccaggccgac gatgtcctgc cggtccattt tcgctgtacg
     1561 atgtcccgac gttgccgtgt gcagaggaac ggagagggtg aaacacgttc ccgtcccgat
     1621 tttgctttgt acggtgaggg agccgcccat cagctcggcg agccgcttgc tgatcgaaag
     1681 gccgagtccc gtccctcccg cgtcgcgtcc ggcctcggac tggcggaatg cgtcgaagat
     1741 gtgctccaaa tccttctccg gaatgcccgg tcccgtatcc tctatctcca cgaggaagcg
     1801 cacggtatcc tcagcgtcgt gctccttgtc cgcacgggcg cgtacggcga tgccgccggt
     1861 tttggtgaac ttggcggcgt tcccgagcag gttgaagagt atctgccgca gttt
//
LOCUS       DAOTIL010000554         1909 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminobacteriaceae bacterium isolate AMR_MDS_3026
            NODE_71525_length_1909_cov_2.654261, whole genome shotgun sequence.
ACCESSION   DAOTIL010000554 DAOTIL010000000
VERSION     DAOTIL010000554.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868050
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminobacteriaceae bacterium (wastewater metagenome)
  ORGANISM  Aminobacteriaceae bacterium
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae.
REFERENCE   1  (bases 1 to 1909)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1909)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:19:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,224
            CDSs (total)                      :: 3,198
            Genes (coding)                    :: 3,184
            CDSs (with protein)               :: 3,184
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1909
                     /organism="Aminobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_71525_length_1909_cov_2.654261"
                     /isolate="AMR_MDS_3026"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:3034609"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1143
                     /gene="xseA"
                     /locus_tag="P5201_10690"
     CDS             <1..1143
                     /gene="xseA"
                     /locus_tag="P5201_10690"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: protein motif:HMM:TIGR00237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009318 - exodeoxyribonuclease VII
                     complex [Evidence IEA];
                     GO_function: GO:0008855 - exodeoxyribonuclease VII
                     activity [Evidence IEA];
                     GO_process: GO:0006308 - DNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII large subunit"
                     /protein_id="HRV99051.1"
                     /translation="VRGELSELKKHSSGHVYFTLLGKESRISCALFRSYASYVPEWPR
                     NGDEVVAEGSVSVYAPRGGYQLLVKRLVPLGKGAADRARLELMARLEAEGLFSPGLKR
                     PFPSYPARVAVVTSPTGAALRDVIAVASKRMPSCELVIIPAAVQGYEAPEAIAEALGR
                     IGSVAGAECAMLVRGGGSRDDLSAFDDERVVRAVRNSPVPLVTGVGHEIDETLSDLAA
                     DLHAPTPSAAAERIFPDRRDILRLLDHRLASARAALELDMSKSLRGIDALLSRGARTL
                     EKDIMTRNASLQLSSSRLFSAAERALSESRERLAACAASLDGLSPLKAFDRGYIVCEK
                     KGVPVLRAAELSSKDEVCLRFRDGDANAKIRSSSVRPVSQGDAADS"
     gene            1164..>1909
                     /gene="mtnA"
                     /locus_tag="P5201_10695"
     CDS             1164..>1909
                     /gene="mtnA"
                     /locus_tag="P5201_10695"
                     /EC_number="5.3.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013048419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046523 -
                     S-methyl-5-thioribose-1-phosphate isomerase activity
                     [Evidence IEA];
                     GO_process: GO:0019509 - L-methionine salvage from
                     methylthioadenosine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S-methyl-5-thioribose-1-phosphate isomerase"
                     /protein_id="HRV99052.1"
                     /translation="MLPRVISWEENRLSLLDQRRLPSSVEFLKCEAAEEVAVAVETLA
                     VRGAPAIGIAAAFGVALEARRGRAEALRAVQRLAVTRPTAVNLFWALRRVEERMKTVP
                     AEASNETLFSAVLEEATAIQREDLEGNRALGRAGAALLPDDCTVMTHCNAGALATGGH
                     GTALGVIRSAREAGKTVRVYACETRPLLQGARLTVWELMQDGFDVTLLCDNMAASLMK
                     SRRIDAVIVGADRIAANGDTANKIGTYSHAL"
BASE COUNT          322 a          539 c          666 g          382 t
ORIGIN      
        1 gttcgcggcg agttgagcga actgaagaag cattccagcg gtcatgtgta tttcaccctc
       61 ctggggaaag aaagccgtat ttcgtgcgct cttttcagaa gttatgcttc gtatgtcccc
      121 gagtggcccc gtaacgggga cgaggtggtc gccgaggggt cggtttccgt atatgctcct
      181 cgcggagggt accagctcct cgtgaagcgg ctggtaccgc tgggaaaggg cgccgcggac
      241 cgcgcacggt tggagctgat ggcgcgcctt gaggcggaag ggcttttctc cccgggtttg
      301 aaacgtccgt ttccctccta ccctgcgcgt gtcgccgtgg tgacgtcgcc gacgggggcc
      361 gcgcttcgcg acgtgatcgc ggtggcttcc aagcggatgc cttcctgcga gctggtgatt
      421 atccccgcgg cggtgcaggg gtatgaagcg cctgaggcca tcgccgaagc gttggggcgt
      481 atcggctctg tcgcgggggc ggagtgcgcc atgttggtcc ggggaggcgg aagcagggac
      541 gacctgtcgg ccttcgacga cgaaagagtc gtccgggctg tccgcaattc tcctgttccg
      601 ctggtgactg gagtggggca tgagatcgac gaaacactct ccgacctggc ggccgatctt
      661 catgcgccga ccccttccgc ggctgccgag cgtatttttc ccgaccggcg ggatattctt
      721 cgtctcctcg atcaccggct tgcatcggcg cgggcggccc tcgaactgga catgtcgaag
      781 agtctgcgcg gcatcgatgc gctgctttca cgcggggcga gaactcttga gaaggacatc
      841 atgacgagga acgcatcgct ccagctctct tcgagcaggc ttttttcggc tgcggagcgt
      901 gcgctctcgg agtccagaga gcggctggcc gcgtgcgccg cttctctcga cgggctctct
      961 ccgttgaaag ctttcgaccg ggggtatata gtctgcgaga agaagggcgt ccccgtgctc
     1021 agggcggccg agctctcatc caaagacgaa gtgtgtctcc ggttccgcga cggggatgcg
     1081 aacgcgaaga tccgttcttc cagcgtgcgc cctgtttccc aaggggatgc tgcggattcc
     1141 taacgtaaag aaggaggggt gacatgctgc cccgggttat ttcatgggaa gaaaacagac
     1201 tgtctcttct tgaccagagg aggctgccgt cgagcgtaga gtttttgaag tgtgaagccg
     1261 ccgaggaggt cgccgtagcg gtagaaacgc tcgctgtgcg aggcgcgcct gcgattggta
     1321 ttgccgcggc cttcggcgtc gcgctggaag cccgcagagg gcgagcggag gcgcttcgcg
     1381 ccgttcagcg tcttgccgta acgcgtccga cggcggtgaa cctcttttgg gcgcttcggc
     1441 gcgttgagga gagaatgaaa accgttccgg cggaggcttc gaacgaaacg ctcttttcgg
     1501 ccgttctgga ggaagcgact gctattcaga gggaggatct ggaggggaac cgcgccctcg
     1561 gccgcgcggg cgccgcgctg cttccggacg actgcaccgt catgacgcac tgcaacgcgg
     1621 gagcgctggc gaccggagga cacggcaccg ccctcggagt aatccgttcg gctcgggagg
     1681 cggggaaaac tgtgcgcgtg tatgcgtgcg agacccgtcc gctgctccag ggggcgcgac
     1741 tcaccgtctg ggagttgatg caggacggtt tcgacgtgac gctgttgtgt gataatatgg
     1801 cggcaagcct tatgaagagc agacgaatcg acgctgtgat cgtgggcgcg gacagaatcg
     1861 cggccaatgg cgacacggcg aataagatcg ggacctattc gcatgcgct
//
LOCUS       DAOTIL010000555         1908 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminobacteriaceae bacterium isolate AMR_MDS_3026
            NODE_71559_length_1908_cov_3.343767, whole genome shotgun sequence.
ACCESSION   DAOTIL010000555 DAOTIL010000000
VERSION     DAOTIL010000555.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868050
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminobacteriaceae bacterium (wastewater metagenome)
  ORGANISM  Aminobacteriaceae bacterium
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae.
REFERENCE   1  (bases 1 to 1908)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1908)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:19:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,224
            CDSs (total)                      :: 3,198
            Genes (coding)                    :: 3,184
            CDSs (with protein)               :: 3,184
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1908
                     /organism="Aminobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_71559_length_1908_cov_3.343767"
                     /isolate="AMR_MDS_3026"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:3034609"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..740
                     /gene="dctP"
                     /locus_tag="P5201_10700"
     CDS             <1..740
                     /gene="dctP"
                     /locus_tag="P5201_10700"
                     /inference="COORDINATES: protein motif:HMM:NF015445.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="TRAP transporter substrate-binding protein DctP"
                     /protein_id="HRV99053.1"
                     /translation="FISVEDPSYVGDYVPDFIALVGPMLYNSYDEYVEIIKTDLVQDM
                     IKRAEEKGIKILALEYVFGFRNVITNKVVKTPEDLKGVKLRTPGSQLFIQTLTAMGAT
                     VTPLPWAETLPGVQQGIVDGIEGSEFTNIGTKVYELLKNVARTQHFLGTCGVYISTQV
                     WEKIPEEYRAIIAEEFAKGALEMVEIGKRDLGDVTAKLESYGVKFNEVDKKAFTEATK
                     SIYETFPGLTPGIYDKLQAELEKIRAK"
     gene            847..1344
                     /locus_tag="P5201_10705"
     CDS             847..1344
                     /locus_tag="P5201_10705"
                     /inference="COORDINATES: protein motif:HMM:NF016200.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter small permease"
                     /protein_id="HRV99054.1"
                     /translation="MKKIVHTLFSYAEEIVSAFFISITVCMVIVNVFFRYVLNMGIFW
                     SEEVATISFVWSVFIGAAACHKRKMHISIDLLTKLAPNFLKKPLRLMAHTIMIILNGY
                     IVYLSVIFVRASSIKPTAVLGISSSYVSSALLVGFGLMTLHSAVFFFEELRSGALPPE
                     QAAQG"
     gene            1350..>1908
                     /locus_tag="P5201_10710"
     CDS             1350..>1908
                     /locus_tag="P5201_10710"
                     /inference="COORDINATES: protein motif:HMM:NF018513.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter large permease subunit"
                     /protein_id="HRV99055.1"
                     /translation="MEFLPVIVVFVLYFSGIPIAFALFGAALSYFTFINTGSPVDLIL
                     QRFVTSTASFPLLAVPFFIMAGSIMNYAGISSRLMKMADVLTGHMTGGLAQVNVVLST
                     LMGGVTGSANADAAMQSKILVPEMEARGYDRAFSAAITAASSAIAPVIPPGINLIIYA
                     LIARVSVDKIFLAGYVPGLIMCCTLM"
BASE COUNT          393 a          576 c          529 g          410 t
ORIGIN      
        1 acttcatctc ggtcgaggac ccctcctatg tcggcgacta cgttcccgat ttcatcgcgc
       61 tggtcggccc gatgctttac aactcctacg acgagtacgt cgagatcatc aagaccgacc
      121 tggtccagga catgatcaag agggccgagg agaaggggat caagattctc gcgcttgaat
      181 acgtcttcgg cttccgcaac gtcattacca acaaggtcgt caagacgccc gaagacttga
      241 aaggcgtgaa gctgcgcacc ccgggaagcc agctcttcat ccagacgctc acagcgatgg
      301 gcgcgaccgt gaccccgctc ccctgggccg agactcttcc gggagtgcag cagggaatcg
      361 tcgacggtat cgaaggctcc gagttcacca acatcggcac caaggtgtac gagctgctga
      421 agaacgtcgc ccgcacccag cacttcctcg gcacttgcgg cgtctacatc tccacgcagg
      481 tatgggagaa gatccccgag gagtaccggg cgattatcgc cgaggagttc gcgaagggcg
      541 cgcttgaaat ggtggagatc ggcaagagag atctcggcga tgtcactgcg aagcttgaat
      601 cgtacggcgt gaagttcaac gaggtcgaca agaaggcctt taccgaggcg acgaagagca
      661 tctacgagac gttccccggc ctgacgcccg gcatctacga caagctgcag gcggagctgg
      721 agaagatccg ggcgaagtag tcgccggatt tccttcacgc gaatgaaata aacgtacggc
      781 gcggcaggga gagggaacgt ggtttctctc cctgccgctg attcactcga cgagaggtta
      841 tcgaccatga aaaaaatcgt acatacactc ttcagctatg cggaggaaat cgtcagcgca
      901 ttcttcatca gcattaccgt ctgcatggtc atcgtcaacg tctttttccg ttacgtgctc
      961 aacatgggga tcttctggtc cgaagaggtc gcgaccatct ctttcgtctg gagcgtcttc
     1021 ataggggccg cggcgtgtca taagcgcaag atgcatatca gcatcgacct tctgacgaaa
     1081 cttgccccga acttcctgaa gaagccgctt cgcctgatgg cgcacacgat tatgatcatc
     1141 ctcaacgggt acatcgtcta tttaagcgtg atcttcgtcc gggcctcttc gataaaaccg
     1201 accgccgttc tgggaatctc ctcaagctat gtgagctcgg cgctcctggt cggcttcgga
     1261 ctgatgacgc tccattccgc cgtcttcttc ttcgaagagc ttcgcagcgg cgctctcccg
     1321 ccggaacagg ctgcgcaggg gtgatgcaga tggaatttct ccctgtcatc gtcgtcttcg
     1381 tgctctactt ttcgggcatc cccattgcgt ttgctctctt cggcgcggcg ctgtcctatt
     1441 tcaccttcat caatacaggc agccccgtcg acctgatcct ccagcgcttt gtcacgagca
     1501 cagcctcctt cccgctcctg gccgtaccct tcttcatcat ggcgggatcg atcatgaact
     1561 acgcagggat aagctcccgc ctgatgaaga tggccgacgt ccttaccggg catatgacgg
     1621 gagggctggc gcaggtcaac gtggtgctca gcacgcttat gggaggcgtc acggggtctg
     1681 caaatgccga cgccgccatg cagagcaaaa tactggttcc cgaaatggag gccagggggt
     1741 acgaccgcgc cttttcggcg gccatcacgg cggcctcttc ggcaatcgcg cctgtcattc
     1801 ctccggggat caacctgatc atctacgccc tgatcgcgcg cgtctcggtc gacaaaatct
     1861 tcctggcggg gtacgtcccc ggcctcatca tgtgctgtac tctcatga
//
LOCUS       DAOTIL010000556         1907 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminobacteriaceae bacterium isolate AMR_MDS_3026
            NODE_71601_length_1907_cov_4.043737, whole genome shotgun sequence.
ACCESSION   DAOTIL010000556 DAOTIL010000000
VERSION     DAOTIL010000556.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868050
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminobacteriaceae bacterium (wastewater metagenome)
  ORGANISM  Aminobacteriaceae bacterium
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae.
REFERENCE   1  (bases 1 to 1907)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1907)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:19:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,224
            CDSs (total)                      :: 3,198
            Genes (coding)                    :: 3,184
            CDSs (with protein)               :: 3,184
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1907
                     /organism="Aminobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_71601_length_1907_cov_4.043737"
                     /isolate="AMR_MDS_3026"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:3034609"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..369)
                     /locus_tag="P5201_10715"
     CDS             complement(<1..369)
                     /locus_tag="P5201_10715"
                     /inference="COORDINATES: protein motif:HMM:NF014518.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009424 - bacterial-type flagellum hook
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar cap protein FliD N-terminal
                     domain-containing protein"
                     /protein_id="HRV99056.1"
                     /translation="MSEYQSTFSIPGISSQIDWGSMADKLLENARKPIEQWTAQQDTI
                     ELKIGLFDEFSASLKSLRSVVSPLKLESVFKAKAAEFTALGGVEASSVVTATVDASAA
                     IARHDIEVTQKAVAETRFSTQ"
     gene            553..1152
                     /locus_tag="P5201_10720"
     CDS             553..1152
                     /locus_tag="P5201_10720"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRV99057.1"
                     /translation="MRVVIDGDEHVFSEDIVSKEDLFSGVKEEVARRGRVVRSITVDG
                     TVLEEDAFLALSGGVEARFETVPVRELVMDSLSEGTRYMSHLSAGLSKVADMLEADKT
                     AEGLDLLRQAAEGIGWSLQVMHNCQILLGISDHEVGDGKVGELKTTLVQDLEKASSSI
                     ENGKHLELSFRIRTGVLPGIQRMAQYMEALLETGRKAVQ"
     gene            1369..>1907
                     /locus_tag="P5201_10725"
     CDS             1369..>1907
                     /locus_tag="P5201_10725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013048736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flippase-like domain-containing protein"
                     /protein_id="HRV99058.1"
                     /translation="MTLRKGLVLFLLLSFGVSALVLASSVDADTWRTITNADGRLLLL
                     ALVFVGAAWCCDAARFCSLARAAGEKIGFRLGMVLTWLHYFGCAVTPMQSGGGPFQVY
                     VLYKKQVPLGKGIAITLTRTLLTLLILGLVVPVAVFIEPDLLKGKLFLKGVFSYVILF
                     IVVSWVLVLLSILRPLLIK"
BASE COUNT          409 a          467 c          505 g          526 t
ORIGIN      
        1 ctgcgtgctg aagcgggttt ctgcaacggc cttctgcgtc acctcgatat catgtcgggc
       61 gattgcggct gatgcatcca cagtcgctgt gacgacgctg ctcgcctcga cacctccaag
      121 agcggtaaat tccgcggctt ttgccttgaa gactgattcc agcttcaaag gcgagaccac
      181 gctgcgaagc gacttcaagc ttgcggaaaa ttcatcgaac aatccgatct tgagctcgat
      241 cgtatcctgt tgggccgtcc actgttcaat cggcttccgt gcgttttcta aaagtttgtc
      301 cgccattgac ccccagtcga tctgcgagga gatcccgggg atcgaaaaag tcgactgata
      361 ttcggacata tataatcatc tccctgtcga agggtattcg ttctcccttt cttatcggca
      421 gagttgctcc cggtcttgac gcttttttct cctctcttgg aaaaagtcat ggtacaatgt
      481 cttccggcaa gattatttgc ggacggcatg gcgaaaagcg cctgtctctg tttttctaaa
      541 aggagggacg ttatgagagt tgtcatcgat ggcgacgaac acgttttttc cgaggatatc
      601 gtctccaaag aagatctttt ttccggagta aaagaagaag ttgccagaag gggaagggtg
      661 gtccgctcta ttaccgttga cggaacagtt ttagaggagg atgctttttt agccctctcc
      721 ggaggagtgg aagcccggtt tgaaaccgtc ccggtacggg aacttgtcat ggattcgctt
      781 tccgagggaa ctcggtacat gagccatctt tccgcagggc tttccaaggt tgcggatatg
      841 ctggaagccg ataaaacagc cgaaggtctg gaccttttac ggcaggccgc tgaaggtatc
      901 ggatggtctt tacaggtgat gcataactgt caaatccttc tgggaatctc tgaccacgaa
      961 gtgggcgacg gaaaggtcgg cgagctcaaa acgacccttg tacaggacct ggaaaaagca
     1021 tcctcatcca tcgaaaacgg gaaacatctg gagctttcgt ttcgtatccg tacaggcgtg
     1081 cttcctggaa ttcagagaat ggcgcagtac atggaagctc ttttggaaac cggccgaaaa
     1141 gcagtgcagt aactcttttc aaaacgccag gcataacttt tttgacgtct ccgtgataac
     1201 gggccggaaa acattctttg agtgttgatc cggagagcgg tcgttttctt tttaagaacg
     1261 cttttctaga cgaaaaaatc tgctacacta tcccccgatt ccgcattata tccgccctgg
     1321 cgcggagagc tgcggggcag ccggaacctg tgaggaggca cttctgtatt gacccttcgt
     1381 aaaggactcg ttctgtttct gcttctttct tttggcgtca gcgcgcttgt tctcgcttca
     1441 agcgtcgatg ccgatacgtg gcggacgata acaaatgccg acgggcgctt gcttttgctg
     1501 gcgcttgtct ttgtcggagc cgcctggtgc tgtgatgccg cccgtttctg ctcccttgcg
     1561 cgtgccgcag gcgagaagat cggttttcga cttggaatgg tgcttacgtg gctgcactac
     1621 ttcgggtgtg cagtaacgcc gatgcaaagc ggcggcggcc ctttccaggt gtatgtgctt
     1681 tacaaaaaac aggtgcctct cggcaaaggc attgcgataa ctctgaccag aacgctgctt
     1741 actcttttga tccttgggct tgtagttcca gtagcagtct ttatcgagcc tgatcttctt
     1801 aaagggaaac tttttttaaa aggcgttttt tcctatgtga ttctttttat tgttgtatcg
     1861 tgggttttgg tgctcctgag cattctgcgc cctctgctga taaaaaa
//
LOCUS       DAOTIL010000557         1903 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminobacteriaceae bacterium isolate AMR_MDS_3026
            NODE_71831_length_1903_cov_2.161797, whole genome shotgun sequence.
ACCESSION   DAOTIL010000557 DAOTIL010000000
VERSION     DAOTIL010000557.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868050
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminobacteriaceae bacterium (wastewater metagenome)
  ORGANISM  Aminobacteriaceae bacterium
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae.
REFERENCE   1  (bases 1 to 1903)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1903)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:19:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,224
            CDSs (total)                      :: 3,198
            Genes (coding)                    :: 3,184
            CDSs (with protein)               :: 3,184
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1903
                     /organism="Aminobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_71831_length_1903_cov_2.161797"
                     /isolate="AMR_MDS_3026"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:3034609"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1903)
                     /gene="dnaE"
                     /locus_tag="P5201_10730"
     CDS             complement(<1..>1903)
                     /gene="dnaE"
                     /locus_tag="P5201_10730"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013048131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit alpha"
                     /protein_id="HRV99059.1"
                     /translation="KITIGPGRGSAAGSLVAWSLKITELDPLKYDLLFERFLNPERIS
                     MPDIDTDVSDKGRDELLHYITEKYGHDKVSQIVTFGRLKSRAAVTDVGRVLGMPVRDV
                     VAITRLIPPMGIHSIGEAVEQVPELAELKKSNPMVARVLDVASNIEGLARHCSQHAAG
                     VVITPVPITDLVPVRRIGQDQIVTQYPMEPIEKLGLVKMDFLGLRTLSVIEEALQNIS
                     LGGKSLPDMERIPLDDRKTYEMLQKADTLGVFQLESTGMRQLLKKLQVDCFEDLIAVL
                     AMYRPGPLGSGMVDQYIECKHGRATVKYLHPLLEGVLRETYGVVLYQEQVMQCAAILA
                     GYSLGEADLLRRAMGKKKADVMEQQRAKFVEGCTQRNINEKSAQEIFDIIQEFAGYGF
                     NKSHSAAYALITYQTAWLKANYRSEFMAAYLSSQIGSKKEDLAAYVREVRNSGIDVLP
                     PDVNSSMASFTPVGEVVRFGLGAVAKAGHTAVEAILAARNTGTPFLSMWDFLCRVDLR
                     VVNKAVVENLVRAGAFDNLENNRRKLLKGLPELISAAQKKCANSNQCSLFDSLPEEDA
                     AGPDLPDVSDFTLLERLEMEKDAVGLYISGHPFEQYEKKVRMLSNCTIAEVRSWRSKR
                     IPPLVGGLVL"
BASE COUNT          346 a          569 c          600 g          388 t
ORIGIN      
        1 agaggacaag gccgccgacc agcggaggta tgcgcttgct cctccatgac cgcacttcgg
       61 caatggtaca gttcgagagc atacgcacct tcttctcgta ctgctcgaaa gggtgtccgg
      121 agatatataa ccccacggca tccttctcca tttcaagacg ttcaagcagc gtgaaatcgg
      181 agacgtccgg gagatcgggc ccggcagcgt cctcctcggg gagcgaatcg aagagcgaac
      241 actggttgct gttcgcacac ttcttctgcg ccgccgagat gagctcgggc agccctttaa
      301 ggagcttgcg tcggttgttt tccaggttgt cgaaggctcc ggcccggaca aggttctcca
      361 cgacggcctt gttgacgacg cgcaggtcga cccggcagag gaagtcccac atcgagagga
      421 agggggttcc cgtgttccgt gcggcaagaa tcgcctcgac tgcggtgtgt cccgccttcg
      481 cgaccgctcc cagaccgaac cggacgacct cgccgaccgg cgtgaagctg gccattgagg
      541 agttgacgtc gggcggaagg acgtcgattc cggagttccg gacctccctg acgtacgcgg
      601 ccaggtcttc cttcttggag ccgatctggc tggagaggta ggcggccatg aactccgacc
      661 ggtagttcgc cttgagccac gcggtctggt aggtgatcaa cgcataggct gcgctgtgcg
      721 atttgttgaa gccgtaaccg gcaaactcct ggatgatgtc gaagatctcc tgcgccgatt
      781 tttcgttgat atttcgctgc gtacaccctt cgacgaactt cgcgcgctgc tgctccatga
      841 cgtccgcctt cttcttgccc atggcgcgcc ggagaaggtc ggcctcgccg agggagtacc
      901 cggcgagtat ggcggcgcac tgcatcacct gctcctggta gaggacgacg ccgtaggtct
      961 cgcgcagcac gccttcgagc agcggatgga gatacttcac cgtagcgcgt ccgtgcttgc
     1021 actcaatata ctggtccacc attccgctgc cgagggggcc gggacggtac atggcgagca
     1081 cggcgataag gtcctcgaaa cagtccacct ggagcttctt gagcagctgg cgcattccgg
     1141 tcgattcaag ctgaaagact cccagagtgt cggccttctg cagcatttcg taggtcttcc
     1201 tgtcgtcgag cgggatgcgc tccatgtcgg ggagcgactt cccccccagc gagatgttct
     1261 gcagcgcctc ttcgatgacg gagagcgtcc ggagtccgag gaagtccatc ttgacgaggc
     1321 cgagcttctc gatgggctcc atcggatact gcgtgacaat ctggtcctgg ccgatgcggc
     1381 gaacggggac gagatcggta atagggacgg gggtgatcac cactccggcc gcgtgctgcg
     1441 aacagtggcg ggcaagccct tcgatgttgg aggcgacgtc gagcacgcgc gccaccatcg
     1501 ggttgctctt tttcaactcg gcaagctcgg gaacctgctc gacggcctcg ccgatggagt
     1561 ggattcccat cgggggaatg agccgcgtga tggcgacgac gtcgcgcaca ggcatgccga
     1621 gcaccctgcc gacgtcggtc accgccgccc tgctcttaag acgtccgaac gtgacgatct
     1681 gcgatacttt gtcgtgtccg tacttctcgg tgatgtaatg aagcagttcg tcgcgcccct
     1741 tgtcggagac gtccgtatcg atatcgggca tgctgatacg ctcggggttg aggaagcgct
     1801 caaagagcag gtcgtatttg agcgggtcga gctcggtaat cttgaggctc caggccacaa
     1861 gcgagcccgc ggcggagcct cgtcccggac ctatcgtaat ctt
//
LOCUS       DAOTIL010000558         1902 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminobacteriaceae bacterium isolate AMR_MDS_3026
            NODE_71853_length_1902_cov_4.340552, whole genome shotgun sequence.
ACCESSION   DAOTIL010000558 DAOTIL010000000
VERSION     DAOTIL010000558.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868050
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminobacteriaceae bacterium (wastewater metagenome)
  ORGANISM  Aminobacteriaceae bacterium
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae.
REFERENCE   1  (bases 1 to 1902)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1902)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:19:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,224
            CDSs (total)                      :: 3,198
            Genes (coding)                    :: 3,184
            CDSs (with protein)               :: 3,184
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1902
                     /organism="Aminobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_71853_length_1902_cov_4.340552"
                     /isolate="AMR_MDS_3026"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:3034609"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..691
                     /locus_tag="P5201_10735"
     CDS             <1..691
                     /locus_tag="P5201_10735"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRV99060.1"
                     /translation="VSIDITPLFTYWADNENHGFIIIADEPLSTSPAHLAFSTPYNND
                     DETDEKPAILITSGETTTTELISSSDHISDLSARPEFENDPALFTLGDGYVPVMVIDY
                     SPLDYKWEILEATLSFTADPLFMKNYFGLDSALPDEAEEKIYCYTVENEDPSDFTFDK
                     YGSTVPGYDADTSRFTLDITGSLRHAFENNQPIGLVPPHYRNQLREMAMNDATIHIVY
                     RKNVEEYEVNE"
     gene            688..1866
                     /locus_tag="P5201_10740"
     CDS             688..1866
                     /locus_tag="P5201_10740"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRV99061.1"
                     /translation="MKRTLLLSIAALFAVLPLFGGSFLALNSFGKYNLQGDIRSFSMG
                     VSGVALTDDYNTSLVNPATATGKDRIFINIGETYSSLSIDYDRFTGFDPSSADFDTPY
                     LNIIFPLPMKGSVLTASYYSPVNSIIELHYTNGTEEIEEVHEKNINFAEFGAAYELLP
                     SLSVGAGALYAFGNERNEYTITDETYTEETTYNKWKYTYSGIGFHLGALYRSDFANIG
                     LSYYPAFDITVSRSFNDARIDPETSFTYPAQWTMGFSLTPGALTLTGEWTAIDWSGID
                     YNNYVLDKMSRLSFGAEYDLKIPYRRHGNLRTFDIPVRAGFYTQKGFYEDGKEWGLTG
                     GFSLFPFKSVSGARMDVLLSYGQRKTLLEDAFQGGLYDYKENFFTIGVSFSATDKWYK
                     "
BASE COUNT          439 a          605 c          426 g          432 t
ORIGIN      
        1 tgtttctatc gatatcaccc ccctcttcac ctactgggcg gacaacgaga accacggatt
       61 catcatcatc gccgacgagc ccctctccac gagcccggct catctggctt tctccacgcc
      121 ctacaataac gacgacgaga ccgacgagaa gccggccatc ctcatcacat cgggagagac
      181 caccaccacg gagctcatca gctcctcgga ccatatctcg gatctgtccg cccgtcctga
      241 atttgaaaac gacccggcgc tcttcaccct gggcgacggt tatgtccccg tgatggtcat
      301 cgactactct cccctggatt acaagtggga gatcctggaa gccaccctct ccttcaccgc
      361 agacccgctg ttcatgaaga actatttcgg actggactcc gctctgccgg acgaagctga
      421 ggaaaagatc tactgttaca ccgtggaaaa cgaagacccg agcgatttta catttgacaa
      481 atacggcagc accgttcccg gttatgatgc agacacgtcc cgcttcaccc tggatatcac
      541 cgggtccctg cgccatgcct ttgagaataa ccagcccatc ggcctggtgc ccccccatta
      601 ccgcaaccag ctgcgcgaga tggcaatgaa tgacgccacg atccatattg tctaccgcaa
      661 aaacgtcgaa gaatatgagg tgaatgaatg aaacggactc ttcttctttc aatagcggct
      721 cttttcgccg ttcttcccct gttcggcggc tcttttctgg ccctcaacag tttcggaaaa
      781 tataatctgc agggcgacat ccgctctttt tccatgggcg tctccggagt cgctctcact
      841 gatgattaca acacatccct ggtcaatccc gccacagcta cgggaaaaga ccggatcttt
      901 atcaatatcg gggagaccta ttccagcctc tccatcgatt acgaccgctt taccgggttt
      961 gacccctcaa gcgccgattt cgacaccccc tacctgaaca tcatctttcc cttgcccatg
     1021 aaaggctcgg tcctcacggc aagctactat tcgccggtga attccataat cgagctgcat
     1081 tatacaaacg ggacggaaga gatcgaagag gtgcatgaga agaacatcaa cttcgccgaa
     1141 ttcggcgccg cgtatgaact cctcccctct ttatccgtgg gagcaggcgc tctttacgcc
     1201 ttcgggaacg agcgcaatga atacaccatc acagatgaga cctacaccga agagaccacc
     1261 tacaacaagt ggaaatacac ctacagcggc atcggttttc acctgggggc cctctaccgc
     1321 tcggattttg ccaacatcgg cctctcctat taccccgcct ttgatatcac cgtctcccgc
     1381 tctttcaacg atgcacgtat cgatcccgag acatccttta cctatccggc tcaatggacc
     1441 atgggcttct ccctcacacc cggcgccctg accctcaccg gcgagtggac agccatcgac
     1501 tggtcaggca tcgactacaa caattatgtc ctggacaaga tgagccgcct ctctttcgga
     1561 gcggaatatg acctgaagat cccttacagg cgccacggca acctgcggac cttcgatatc
     1621 cccgtccgcg ccgggttcta tacgcagaag gggttttatg aagacggcaa agagtggggc
     1681 ctcaccggag gattctccct cttccccttt aaaagcgtga gcggagcccg catggatgtg
     1741 ctcctctcct acggacagcg caagactctt cttgaagacg cttttcaggg cggcctctac
     1801 gattataaag agaatttctt taccataggc gtgagttttt ccgcaacaga taagtggtac
     1861 aagtgatgat tcacttgtac cgcttctatt ctgcgctctc cc
//
LOCUS       DAOTIL010000559         1902 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminobacteriaceae bacterium isolate AMR_MDS_3026
            NODE_71858_length_1902_cov_3.859231, whole genome shotgun sequence.
ACCESSION   DAOTIL010000559 DAOTIL010000000
VERSION     DAOTIL010000559.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868050
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminobacteriaceae bacterium (wastewater metagenome)
  ORGANISM  Aminobacteriaceae bacterium
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae.
REFERENCE   1  (bases 1 to 1902)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1902)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:19:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,224
            CDSs (total)                      :: 3,198
            Genes (coding)                    :: 3,184
            CDSs (with protein)               :: 3,184
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1902
                     /organism="Aminobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_71858_length_1902_cov_3.859231"
                     /isolate="AMR_MDS_3026"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:3034609"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..701
                     /locus_tag="P5201_10745"
     CDS             <1..701
                     /locus_tag="P5201_10745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005659479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="(Fe-S)-binding protein"
                     /protein_id="HRV99062.1"
                     /translation="EYVVSVRKVIRDLLACNVKVTGTKTKAISRRWLLWSISGGESRH
                     ISSFFDKGFTSISVSGLRNTQDLLNELELGRLAGVDFIECRACDLGCIGGTGTYESRF
                     LSQLRLDNIEAEWMPSGEEMEQIRSWYDKGIWRLAAPVTAKERLPLSKDIKEAMAKLR
                     EMDAIYAELPHIDCGACGRPSCRALAEDIVRGQGEVTDCVFKLRERITNLSGEIKNLS
                     SKLPHTLHPGRKRR"
     gene            707..1036
                     /locus_tag="P5201_10750"
     CDS             707..1036
                     /locus_tag="P5201_10750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013048076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine kinase"
                     /protein_id="HRV99063.1"
                     /translation="MKIRDVIEKTGATVLAPGDAERELAGAISGDLLSFIMAEAKEGW
                     LWITIQVHLNVAAVAVLKDVPFILVASGRAPEQALVERCAAEGISLAVTKLPAFEAAG
                     ALRELAR"
     gene            1039..1797
                     /locus_tag="P5201_10755"
     CDS             1039..1797
                     /locus_tag="P5201_10755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008712731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol-phosphatase"
                     /protein_id="HRV99064.1"
                     /translation="MLSHFWVDLHLHTVLSPCGELEMGAPEIVAAYREAGIALCAVTD
                     HNDIANVPAIRGAASGNPVVLFGMEVQTAEDIHVVVLFPDDRTAEGYREWLWREMPQR
                     ANDPDIFGDQLVIDSENAILDEQQILLVQGVGYSVDEVASEAYERGALVIPAHIDRPS
                     FSYESVLGRIPDEFPCHALELSPAVSHAAFSEWKARYPERVFIRSSDSHRLSTIARDR
                     CTVMLLEAPSFEEIAMALNGQEGRSVLCPWVGAP"
BASE COUNT          356 a          555 c          623 g          368 t
ORIGIN      
        1 tcgagtacgt ggtgagcgta cggaaggtga tacgggacct cctggcctgc aacgtgaagg
       61 tgacgggcac gaagacgaag gcgatcagca ggagatggct tctttggtcg atctccggcg
      121 gcgagtcgcg tcacatctcc tctttcttcg acaaggggtt cacgtcgatc tccgtctcgg
      181 gcttgcggaa cacgcaggac ctcctcaacg aacttgagct cggccgcctt gcgggcgtcg
      241 acttcatcga gtgccgcgcg tgcgacctcg gatgtatcgg cggcacgggg acgtacgagt
      301 cccgttttct ctcccagctc cgccttgaca acatcgaagc cgaatggatg ccctcggggg
      361 aggagatgga gcagatccgg tcgtggtatg acaagggcat ctggcgtctt gccgcgccgg
      421 tgaccgcaaa agagcgcctg ccgctctcca aggatatcaa agaggcgatg gcgaagctgc
      481 gcgagatgga cgccatctac gcggagcttc cccatataga ctgcggagcg tgcggccgcc
      541 cgtcgtgcag ggcgcttgcg gaggatattg tccggggaca gggagaagta accgactgcg
      601 ttttcaagct ccgtgagcgg ataaccaatt tgagcggcga gatcaagaat ctctcctcga
      661 aactgcccca tacgctccac cccggaagaa aacggaggtg acattgatga aaatacggga
      721 tgtcattgaa aagaccgggg cgacggttct cgctccggga gatgctgaac gcgaacttgc
      781 cggagcgatc tccggcgacc tcctgagttt catcatggct gaagcaaagg aggggtggct
      841 ctggatcacg atccaggtgc acctcaacgt cgctgcggtg gctgtgctga aggatgtgcc
      901 ctttatcctc gtcgcttcgg gaagggcgcc ggagcaagcg ctggtcgaac ggtgcgccgc
      961 cgagggtatt tccctcgccg tgacgaagct ccccgccttt gaagccgcag gggcgctccg
     1021 ggagcttgcg cgctgagcat gctctcgcac ttttgggtag acctgcacct gcataccgtc
     1081 ctctcgccgt gcggcgagct ggagatggga gccccggaga tcgtcgctgc ctacagggaa
     1141 gcaggtatcg ctctctgcgc ggtgaccgac cataacgaca tagcgaacgt tcccgcaatc
     1201 aggggcgcgg cgtctggaaa ccctgtcgtc ctctttggga tggaggtgca gacggcggag
     1261 gatattcacg tcgtggtgct cttccccgac gaccggactg cggaagggta ccgggaatgg
     1321 ctctggcggg agatgcccca gcgtgcgaac gatccggaca tcttcggcga ccagctcgtg
     1381 atcgacagtg aaaacgcgat tctggacgag cagcagatac tgctcgtcca gggagtcggc
     1441 tattcggtcg acgaggtcgc gtccgaggcg tacgaacggg gcgcgcttgt tatcccggcc
     1501 catatcgaca ggccgtcgtt ctcgtacgag tcggtgctcg ggaggattcc cgacgagttc
     1561 ccgtgccacg ccctcgaact ctccccggcg gtgtcccatg ccgcattttc agagtggaaa
     1621 gcgcggtacc cggagagagt cttcatccgc tcttccgatt cccatcgcct tagtactata
     1681 gcgagggatc ggtgcaccgt gatgttgctc gaagcgccct ctttcgagga gattgcgatg
     1741 gcgctgaatg gtcaggaggg gagatccgtg ctctgcccct gggtcggggc gccgtgacgc
     1801 cgtgcaagct ccgcgtagac atccctcggg cttaaacccg ccgccagcag cgcgacctgg
     1861 aggtggtaga gcaagtcggc ggcttcgcag atcgtttcct tc
//
LOCUS       DAOTIL010000560         1902 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminobacteriaceae bacterium isolate AMR_MDS_3026
            NODE_71860_length_1902_cov_3.614510, whole genome shotgun sequence.
ACCESSION   DAOTIL010000560 DAOTIL010000000
VERSION     DAOTIL010000560.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868050
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminobacteriaceae bacterium (wastewater metagenome)
  ORGANISM  Aminobacteriaceae bacterium
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae.
REFERENCE   1  (bases 1 to 1902)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1902)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:19:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,224
            CDSs (total)                      :: 3,198
            Genes (coding)                    :: 3,184
            CDSs (with protein)               :: 3,184
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1902
                     /organism="Aminobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_71860_length_1902_cov_3.614510"
                     /isolate="AMR_MDS_3026"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:3034609"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..203
                     /locus_tag="P5201_10760"
     CDS             <1..203
                     /locus_tag="P5201_10760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006299638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="myristoyl transferase"
                     /protein_id="HRV99065.1"
                     /translation="IVNRLLLRRMLDTILPSIFPEDSSAWTPGVLSLADYERTSEMMK
                     SVFVDMTETAPYDVFFPGGDER"
     gene            200..>1902
                     /locus_tag="P5201_10765"
     CDS             200..>1902
                     /locus_tag="P5201_10765"
                     /inference="COORDINATES: protein motif:HMM:NF014763.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SpoIIE family protein phosphatase"
                     /protein_id="HRV99066.1"
                     /translation="MKERFFPLFPDGRRGVSIAAKLALMIVAGVGAVFLAIVFYGYST
                     SREALEEEFQGRMENLGRAAESQFLQIPMVAEAVTRDLATMITLFEPPPEKMPEMMKE
                     LLASHSQVTGLYVGFSKQATNPLFRNFCPLVFRQGNSIQYKNLAGEMDWTVQDWYQLP
                     LQMGKPLWTEPFFADERAESMMISYGIPLYDANWRYLASIGASLELHWLADIISTMEV
                     GGHGYVFLVTSNGAFVSHPRRELLMRETLFTVAEMENRPDLIPLGHRMIRGEKGVASL
                     YDPLRGDLLVFFRPVGNIGWSLGIVFPEEEVLDDIIHLRRVQTFLGLGGVFAMLLMVF
                     FISNRISGPIRKLKDATMLLASGDLHAPLPPVRGRDEVALLTGSFASMRENLLLHLER
                     LKTETAARERIASELDIARSIQMSLVPRTFPPFPKDGRIDLFAKLEPAREVGGDFYDF
                     FHTDEDHLLLVVGDVSGKGIPAALFMAVTRSFVKALAISSGSCVPPGELMAAVNDRIA
                     EGNDACMFVTLCFVLVDLRDGSFSYACGGHPFPRLLGGDAALPLAPVKGPLVGAMEGM
                     F"
BASE COUNT          323 a          597 c          565 g          417 t
ORIGIN      
        1 atatcgtgaa ccggcttctg ctgcggcgga tgctcgacac gatactcccc tcgatcttcc
       61 cggaggactc ctccgcatgg acgcccggcg ttctgtctct cgccgactac gagcgcacct
      121 ccgaaatgat gaaaagcgtc tttgtcgata tgacggagac agccccttac gacgtctttt
      181 tccccggggg tgacgagcga tgaaggagcg ttttttccct ctctttcccg acggacgcag
      241 aggcgtctcc atagcggcga agctggcgct gatgattgtc gcgggcgtcg gcgccgtttt
      301 cctcgccatc gtcttctacg gctactccac atcgagggaa gcgcttgagg aggagttcca
      361 ggggcggatg gagaacctcg gcagggccgc agagagtcag ttcctccaga ttcccatggt
      421 ggcggaggcc gtgactcgcg atcttgctac catgatcacc ctcttcgagc cgcctccgga
      481 gaagatgccg gagatgatga aggagcttct tgcgtcccat tcgcaggtca ccgggctgta
      541 cgtcggcttt tcaaagcagg cgacgaatcc gctctttcgc aatttctgtc cgttggtttt
      601 ccgacagggg aattccattc agtataagaa ccttgccggc gaaatggact ggacggtgca
      661 ggattggtac cagcttccgc tacagatggg caagcctttg tggacggagc cgttcttcgc
      721 cgacgaacgc gccgaatcga tgatgatcag ttacggtatt cccctctacg acgcgaactg
      781 gcgttatttg gcctctatcg gcgcgtcgct cgaactccac tggctggccg acatcatctc
      841 cacgatggag gtcggaggtc acggctacgt ctttcttgtg acgtccaacg gcgcgtttgt
      901 ctcccatccg cggcgggagc tgctgatgcg cgaaacgctc ttcaccgtgg ccgagatgga
      961 gaaccgcccc gacctcatcc ctctcggcca cagaatgata cggggcgaga agggggtagc
     1021 gtccctgtac gatcccctcc gcggcgacct cctggtcttt ttccgtccgg tgggaaatat
     1081 cggctggtcg ctcggcatcg tctttcccga agaagaggtt ctggacgaca ttatccatct
     1141 gcgccgggtg cagactttcc tcggtctggg gggagtattc gccatgctgc tgatggtttt
     1201 tttcatctcg aacagaatca gcgggccgat ccgaaagctg aaagacgcca ccatgctcct
     1261 cgcctccggc gatctccacg cgccgctccc tccagtgagg ggccgcgacg aggtcgcgct
     1321 gctcaccggc tcgttcgcct ccatgcggga gaatcttctg cttcaccttg aacgtctgaa
     1381 gaccgagacc gcggcgcgcg agcgcatcgc cagcgagctg gacatcgccc ggtccattca
     1441 gatgagcctt gttccgcgca ccttcccccc gttcccgaag gacggccgca tcgatctctt
     1501 cgccaagctc gaaccggcgc gcgaagtcgg cggcgatttt tacgacttct tccacacgga
     1561 tgaagatcat ctgctgctcg tcgtcggcga cgtctccggc aaggggatac ccgccgcgct
     1621 cttcatggct gtgacgcgga gcttcgtcaa ggcgcttgcc atctcctccg gaagctgcgt
     1681 tcctcccgga gagctgatgg ccgccgtcaa cgaccgcatc gccgagggga acgacgcctg
     1741 catgttcgtc acgctctgct tcgtcctcgt ggatcttcgg gacggttcgt tcagctacgc
     1801 ctgcggaggg catcccttcc ccagactgct gggcggcgac gctgcgctgc cgttagctcc
     1861 ggtgaaaggg ccgctcgtcg gggcgatgga gggaatgttt tt
//
LOCUS       DAOTIL010000561         1902 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminobacteriaceae bacterium isolate AMR_MDS_3026
            NODE_71871_length_1902_cov_2.928533, whole genome shotgun sequence.
ACCESSION   DAOTIL010000561 DAOTIL010000000
VERSION     DAOTIL010000561.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868050
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminobacteriaceae bacterium (wastewater metagenome)
  ORGANISM  Aminobacteriaceae bacterium
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae.
REFERENCE   1  (bases 1 to 1902)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1902)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:19:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,224
            CDSs (total)                      :: 3,198
            Genes (coding)                    :: 3,184
            CDSs (with protein)               :: 3,184
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1902
                     /organism="Aminobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_71871_length_1902_cov_2.928533"
                     /isolate="AMR_MDS_3026"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:3034609"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1110)
                     /locus_tag="P5201_10770"
     CDS             complement(<1..1110)
                     /locus_tag="P5201_10770"
                     /inference="COORDINATES: protein motif:HMM:NF021489.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2207 domain-containing protein"
                     /protein_id="HRV99067.1"
                     /translation="MKRLAGVLALLTFLLLPLSAGAQEKIHLFSSRATLLADSSLEVR
                     EDITVNVEGRQIRRGIYRDFPTKYTDSSGRTVRVGFDVLETLLDGREVPFKTESIANG
                     VRVYIGDPDEFAPLGKRTYTLSYVTTHQIGFFEGHDELYWNVTGNGWDFAIDEARFTL
                     SIPGDTPFNSVEFYTGWQGARGQNARVLPDNSVETTAPLGPKEGLTVVYTWPKGIVTP
                     PTIPFYIRFFEKYAFHFIVALPVLLGILYALLWLRWGKDPSMPAIIPLFSASAGRTPG
                     FLRYVRRMGMDNTCFAADILNLAVKGHLVIEELTTEQLAKRSGATGFFGSLAARLAGK
                     TYALKRTESPAPVSPAEKALFSALFAYNKNEILLRQ"
     gene            complement(1128..1682)
                     /locus_tag="P5201_10775"
     CDS             complement(1128..1682)
                     /locus_tag="P5201_10775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013048298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LemA family protein"
                     /protein_id="HRV99068.1"
                     /translation="MIALYVILGIAALVLLWGIGIYNGLIKKVNLKNEAWSGIDVQLK
                     RRFDLIPNLIETVKGYAGHEKEVFLKVTEARSAIGQSKSVAERAESENMLSGALKSLF
                     AVAEGYPELKANTNFLQLQEQLSCLENDIQMSRRYYNGAARDYNIAIATFPAVIVANM
                     FGHSKADYFEAVEEERSAPKVSFQ"
BASE COUNT          386 a          522 c          572 g          422 t
ORIGIN      
        1 ctgccggagc agaatctcgt ttttgttgta ggcgaaaagc gcggaaaaaa gcgccttctc
       61 cgctggggaa accggggcgg ggctctcggt gcgtttcagg gcgtatgtct ttcccgccag
      121 tcgggctgca agactgccga agaagcccgt cgcgccgctg cgcttggcga gctgttccgt
      181 ggtcagctcc tcgataacga gatgcccttt gaccgcgagg ttcagaatat ccgcggcgaa
      241 gcaggtgttg tccataccca ttcggcgaac gtagcgaaga aatccgggcg ttctgccggc
      301 cgaggccgag aagagcggta tgatcgccgg catggatggg tctttccccc agcggagcca
      361 gaggagagcg tacaggattc cgaggagtac aggaagcgcg acgatgaaat ggaacgcgta
      421 cttttcaaaa aaacggatgt agaacggaat tgtgggaggc gtcacgatgc cctttggcca
      481 ggtgtagacg acagtgagcc cttctttcgg gccgagggga gccgttgttt caacgctgtt
      541 gtccggcaga acgcgcgcgt tctgtcctcg cgccccctgc caccccgtgt agaactccac
      601 cgagttgaac ggcgtgtcgc cggggatgga gagggtgaag cgcgcttcgt cgatggcgaa
      661 gtcccaaccg ttgcctgtga cgttccagta gagttcgtca tgtccctcga aaaaaccgat
      721 ctggtgcgtt gtcacgtaag acagcgtgta ggtccgtttg ccgagaggag cgaattcgtc
      781 ggggtcgccg atatacacgc ggactccgtt ggctatcgat tctgtcttga acgggacttc
      841 ccggccgtcg aggagcgttt ccagaacatc gaaacctacc cgcaccgtac gccctgaaga
      901 atctgtatat tttgtaggga aatcgcggta aatgccccgg cgtatctgcc tgccctcgac
      961 gttgacggtg atgtcctcgc ggacttcgag cgaggagtcc gcgaggagcg ttgcccttga
     1021 ggagaaaaga tggatcttct cctgcgcccc ggccgagagc ggcaggagaa gaaatgtaag
     1081 gagcgcgagt actcccgcga ggcgtttcat gatcgcaaac cttccgtcta ttggaacgac
     1141 actttgggcg ccgaacgttc ttcctctacc gcctcgaagt agtccgcctt gctgtgcccg
     1201 aacatgttgg ccacgatgac tgcggggaag gtcgcgatgg cgatattgta gtctctggcc
     1261 gcgccgttgt agtaacggcg ggacatttgt atatcgttct ccaggcagga gagctgttcc
     1321 tgaagctgga ggaagttcgt gttggccttc agttcggggt agccttcagc caccgcgaag
     1381 agggatttca gcgcgccgga gagcatgttc tcggattccg cccgttcggc gaccgacttc
     1441 gactggccta tagcggagcg ggcttccgta accttcagaa aaacctcttt ctcgtgcccc
     1501 gcatacccct tgaccgtttc aatgaggttg gggatgaggt cgaagcgtcg tttgagctgc
     1561 acgtcgatgc cgctccacgc ttcatttttc aggttcacct ttttgatgag accgttgtaa
     1621 atgccgattc cccaaagcag aacgagcgcc gcaataccga gtatcacata cagtgctatc
     1681 atttttttcc ctccttggcg taatcaatgg atttctcacc atcattctac cattcccgga
     1741 aggaagcgca caggaaaata tacgtaccgg ccgcgctaca aaaaacgcct gatctcttcg
     1801 agactttttc gtcgtttcgg gggaaggaac tcctcgtcct cggggtaccc catggacagc
     1861 ctcaggtcga tcttggcgga ggacggaaga tcgcgcgcct tc
//
LOCUS       DAOTIL010000562         1901 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminobacteriaceae bacterium isolate AMR_MDS_3026
            NODE_71903_length_1901_cov_3.299567, whole genome shotgun sequence.
ACCESSION   DAOTIL010000562 DAOTIL010000000
VERSION     DAOTIL010000562.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868050
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminobacteriaceae bacterium (wastewater metagenome)
  ORGANISM  Aminobacteriaceae bacterium
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae.
REFERENCE   1  (bases 1 to 1901)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1901)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:19:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,224
            CDSs (total)                      :: 3,198
            Genes (coding)                    :: 3,184
            CDSs (with protein)               :: 3,184
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1901
                     /organism="Aminobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_71903_length_1901_cov_3.299567"
                     /isolate="AMR_MDS_3026"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:3034609"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..766)
                     /locus_tag="P5201_10780"
     CDS             complement(<1..766)
                     /locus_tag="P5201_10780"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRV99069.1"
                     /translation="MSRSIVTRLALFMIMGMLVLGGALAFLARMGVDELSRAMESTTD
                     RILDEDIRNKVLNDEQTAEAYGQAMAGYLAWIALPPLWDYNYKNLSDYAAGMLNVPNV
                     LAAVIYKEDGSIAAGEMMIGSKNGGKDSRVFTSDIVREGQKIGSVELVLSTAHLEQLR
                     QKSEETREELFASFKANTAATEQSVMRRMFLLSAVILAALLALITFSIIKLVAPLRGM
                     TRAIRRFSETDSFLSSRKSAPSNTHSDRRTSLFSDAL"
     gene            1044..>1901
                     /locus_tag="P5201_10785"
     CDS             1044..>1901
                     /locus_tag="P5201_10785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009202296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IclR family transcriptional regulator"
                     /protein_id="HRV99070.1"
                     /translation="MEKKNGVLLQAPAETKESGVSVPVFSGDDAESVQRESGASIRVL
                     DRAVAVLGFMAEQKRQVGITEIAEATHLSKATVHRILSTLRDYSVVLKDESGRYQMGP
                     SVLYWADAYRKHSGLAELARPYLHTLWEIARETVHLFVFEAGEAYYIDKLDSPHPVGM
                     RSRIGARRDLYSTSGGRAILSFLPEAELKSYLDRTELRPRTRRTITDRKVLEEFLAEC
                     RKRGFCEEVEENEEGIRCVGAAVLDLRGYPVGAVSVSAPAYRFDDRASLATGERIRET
                     AMAISRELGY"
BASE COUNT          381 a          518 c          611 g          391 t
ORIGIN      
        1 cgagcgcgtc gctgaaaagg gatgtccttc ggtcggagtg ggtattcgag ggtgcacttt
       61 tccttgagga caggaaggag tccgtctcgg agaagcggcg tatggcgcgc gtcatcccgc
      121 gcagcggcgc gaccagcttg atgatgctga atgtgatcag cgcgaggagg gcggcgagga
      181 tcaccgccga gagcaaaaac attcttcgca taacggactg ttcggtggcg gcggtattcg
      241 ctttgaagga ggcgaagagc tcttcgcgcg tttcttcgct cttctgccgc agttgctcaa
      301 ggtgtgcggt gctgaggacg agctccacgg agccgatctt ctgcccctcg cgcacgatat
      361 cggatgtgaa cacccgggag tctttcccgc cgttcttcga accgatcatc atctcgcccg
      421 ccgcgatcga accgtcttcc ttgtagatga ccgcggcgag gacgttgggg acgttgagca
      481 ttcctgcggc gtagtcggac aagttcttgt agttgtagtc ccacagggga gggagcgcga
      541 tccaggcgag gtaccccgcc atggcctgtc cgtaggcttc ggcggtctgc tcgtcgttca
      601 gcactttatt ccgtatgtcc tcgtccagaa tgcggtcggt agtggactcc atagcgcggg
      661 agagttcgtc cacgcccatt cgggcgagaa acgccagagc tcctccaaga acaagcatgc
      721 ccatgatcat aaaaagggcg agtcgtgtga cgatacttct ggacatacag cactcctcct
      781 gtccggttgc gcgcaaaaag gcgggagttg cccttgtctt tcggaatacg ataccaaacg
      841 ccggatcttc gcgcagggga gatgcagcgc tggtttcaga cattgtacag cagggaagcg
      901 atcagtgaaa gttttttctt tttgttcgac ttcttggatt caacggaaaa gttccactga
      961 aaaagagttg acatcctcgc ccctgtatcg taccttagtg tcgtttcatc caaagaatcg
     1021 ctattttggc aggtggaatt attatggaga agaagaacgg cgtactgtta caagctcctg
     1081 cggagacaaa ggagagcggc gtcagcgttc ctgttttttc gggagacgac gccgagagcg
     1141 tgcagcggga gtcaggagcc tccatcaggg tgcttgaccg tgcggttgcc gtgctcggct
     1201 tcatggcaga gcagaaacgg caggtaggca tcacggaaat agcggaggcg acgcatcttt
     1261 caaaagcgac ggtgcaccgt attctctcga cgctccgcga ctactccgtc gttctgaagg
     1321 acgagtcggg aaggtatcag atggggccct ccgtcctcta ctgggcggat gcgtaccgga
     1381 aacactcggg gctggccgag ctggcgcgtc cgtatctgca cacgctgtgg gagatcgccc
     1441 gggaaacggt gcatctcttc gtcttcgagg cgggggaggc gtactacatc gacaagctcg
     1501 acagcccgca ccccgtgggc atgcgttccc gcatcggcgc gcggagggac ctctacagca
     1561 cgtccggcgg ccgcgcaatc ctttcgttcc ttcccgaggc ggagctgaaa tcctacctcg
     1621 acaggacgga gctccgtccc cgaaccagga gaaccatcac ggacaggaag gtgctggaag
     1681 agtttctcgc tgaatgccgc aaacgcggct tctgcgagga ggtggaggag aacgaggagg
     1741 ggattcgctg cgtaggggcc gccgtgcttg atctgcgggg ctaccccgtg ggggcggtga
     1801 gcgtgagcgc tccggcctac cgtttcgacg acagagcctc tctggcgaca ggggaacgaa
     1861 tacgggagac ggcaatggca atctcccgcg agctggggta t
//
LOCUS       DAOTIL010000563         1898 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminobacteriaceae bacterium isolate AMR_MDS_3026
            NODE_72056_length_1898_cov_3.308736, whole genome shotgun sequence.
ACCESSION   DAOTIL010000563 DAOTIL010000000
VERSION     DAOTIL010000563.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868050
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminobacteriaceae bacterium (wastewater metagenome)
  ORGANISM  Aminobacteriaceae bacterium
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae.
REFERENCE   1  (bases 1 to 1898)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1898)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:19:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,224
            CDSs (total)                      :: 3,198
            Genes (coding)                    :: 3,184
            CDSs (with protein)               :: 3,184
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1898
                     /organism="Aminobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_72056_length_1898_cov_3.308736"
                     /isolate="AMR_MDS_3026"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:3034609"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..229
                     /locus_tag="P5201_10790"
     CDS             <1..229
                     /locus_tag="P5201_10790"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRV99071.1"
                     /translation="NKVSDLARTQSLSNTEMAEAVNSIARGTSDIVERLEGIRRSTMD
                     AREAFESVTEQANTLLEGMEEMERQLGQFKV"
     gene            complement(298..1101)
                     /locus_tag="P5201_10795"
     CDS             complement(298..1101)
                     /locus_tag="P5201_10795"
                     /inference="COORDINATES: protein motif:HMM:NF012707.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HRV99072.1"
                     /translation="MRRMSLLFLCVAACMLSLGSVARAVEMRIAYEDNEQPPYIYGNS
                     AAIPEKPGLSVEMILMLPEKIPGLEIKLVRMPWLRCIVELENNEVDGIFNASYKKERL
                     RIGRYPTVDGTPDGPVDVDSRIAGMTYSFYVLRKNVGSFQWDGVHFDTIRNAGTIGTP
                     LGYSIAGDLREQGVAVDESGGPETNLAKLAGGRFVAAALLEATVEGLIRSNALYSEIV
                     KVNPPLETKPYYLMLSRAFVEEHPELSRRIWGALREIRDTKTAEILARY"
     gene            1415..1588
                     /gene="lysW"
                     /locus_tag="P5201_10800"
     CDS             1415..1588
                     /gene="lysW"
                     /locus_tag="P5201_10800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009200992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0019878 - lysine biosynthetic process via
                     aminoadipic acid [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysine biosynthesis protein LysW"
                     /protein_id="HRV99073.1"
                     /translation="MSTKVQCVVCEGDVMLPAEVMEGELLTCPDCGTELEVVSLNPLT
                     VAEAPEVQEDWGE"
     gene            1589..>1898
                     /locus_tag="P5201_10805"
     CDS             1589..>1898
                     /locus_tag="P5201_10805"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="L-2-aminoadipate N-acetyltransferase"
                     /protein_id="HRV99074.1"
                     /translation="MAHFFIFYTRLRTEEKLLFKAAEERGIPFSAVNVSDGMWPDIPG
                     ASGDVALCRCVSQTQNSALAVLLEARGVLTVNSSEVMALCADKIVTAAKLSAAGIPQP
                     E"
BASE COUNT          423 a          540 c          519 g          416 t
ORIGIN      
        1 gaacaaggtc tcggacctgg cacgcacgca atcgctttca aataccgaaa tggccgaggc
       61 cgtcaacagc attgcgaggg gaacctccga catcgtggaa cgccttgagg ggatacgccg
      121 ctccaccatg gatgcccgcg aagcgttcga atccgttacg gaacaggcga acacccttct
      181 ggaaggtatg gaagagatgg agcgtcagct gggccagttc aaggtgtaag tcaagccggc
      241 ttttctattg aagagaaagg ggagcggggc tgtttttcgg ccccgctccc ctttttttca
      301 gtacctcgca agaatttccg ccgtctttgt atccctgatt tcccggagag cgccccaaat
      361 tctccgcgag agttccgggt gttcttctac gaaggccctt gagagcatga ggtaatacgg
      421 ttttgtctcc agcgggggat tgactttgac aatttcgcta tagagagcgt tcgagcgaat
      481 caaaccttcg acagtcgctt caaggagcgc cgcagccaca aaccggcctc cggcgagctt
      541 ggccaggttc gtctcgggcc ctccggactc gtcgacggcg acgccctgtt cacggagatc
      601 accggcaatg gagtagccga gcggggtgcc gatagtcccc gcgttcctga tcgtgtcgaa
      661 atggactccg tcccattgaa aggaaccgac gttcttgcgc agtacataga acgaataggt
      721 catgccggcg atcctggaat cgacgtcgac aggcccgtcg ggcgtgccgt cgacggtcgg
      781 gtatcgtccg attctcagcc gttctttttt gtagcttgcg ttgaagatgc cgtccacctc
      841 gttgttctcg agctccacga tacaacgaag ccacggcatg cggacaagct tgatttcaag
      901 ccctggaatc ttctccggaa gcatgaggat catctccacg gaaagccccg gcttttccgg
      961 tatcgccgcc gagtttccat agatataagg aggctgttcg ttgtcttcat acgcgatcct
     1021 catttcgacg gcacgagcaa ccgacccgag agacagcatg caggcggcaa cgcaaagaaa
     1081 taacagcgac atccttcgca tacgaaaaca cctctctttc tcagggtatt gcgctcagtt
     1141 ttcagcgtct tccgattgcc gagcacaaag gttccatggt tttggacgcc ctctaaaaag
     1201 aatattttca ttataacacc aaaaggcgct ttcaattcac aaagtaaaga cctctttcag
     1261 tgcagctcct ccaatgccgc acagagcgta ggattctcgc cggaacaccc agtctaaaat
     1321 acagaaaaaa ggaacattat ttccctactt gacgaaaatc aaactcaggt ttaaaatccc
     1381 tccattgtta aattttgttt tggagggatg ttttatgtca actaaagttc aatgcgttgt
     1441 ctgcgaagga gatgtcatgc tgcccgccga ggtcatggag ggcgagctgc ttacctgccc
     1501 cgactgcgga acggagcttg aagtggtctc cctgaatccg ctcacggtcg ccgaggcccc
     1561 cgaggtccag gaggactggg gagagtagat ggcacacttc ttcatcttct acacccgtct
     1621 ccgcacggag gaaaagctgc tgttcaaagc cgccgaagag cggggaattc ccttctcggc
     1681 ggtgaacgtg agcgacggca tgtggcccga tattcccggc gcttccggcg acgtggcgct
     1741 ctgccggtgc gtgagccaga cgcagaactc cgcgctcgct gtgctgctgg aggcgcgcgg
     1801 cgtgctcacg gtgaactcct ccgaagtaat ggccctctgc gccgacaaga tcgtcaccgc
     1861 cgcgaagctc tccgccgctg gaataccgca gccggagt
//
LOCUS       DAOTIL010000564         1898 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminobacteriaceae bacterium isolate AMR_MDS_3026
            NODE_72060_length_1898_cov_3.229517, whole genome shotgun sequence.
ACCESSION   DAOTIL010000564 DAOTIL010000000
VERSION     DAOTIL010000564.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868050
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminobacteriaceae bacterium (wastewater metagenome)
  ORGANISM  Aminobacteriaceae bacterium
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae.
REFERENCE   1  (bases 1 to 1898)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1898)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:19:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,224
            CDSs (total)                      :: 3,198
            Genes (coding)                    :: 3,184
            CDSs (with protein)               :: 3,184
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1898
                     /organism="Aminobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_72060_length_1898_cov_3.229517"
                     /isolate="AMR_MDS_3026"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:3034609"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..830)
                     /locus_tag="P5201_10810"
     CDS             complement(<1..830)
                     /locus_tag="P5201_10810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009164275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HRV99075.1"
                     /translation="MHLEGRHISPAYEHHVQRKGLFLFGLAAACVLMVVFSISRGAVS
                     IPPLDVVRTLLGGGEAKWRIIVLNIRLPQTLAALLAGGGLSLAGAAMQSVLRNPLGSP
                     FTLGISNAAAFGAAFSIIVLGTGSMQSSAVNAVTIVNPWLTTGSAFIAALLCTGVVLV
                     ITWVTRGTPDVMILAGVALSSLFTAGTMFLQYFADDSQLAATVFWTFGDVSRASWRDI
                     PLMGLLLLGAFCLFFLRRFDLNAIDCGDETALSLGVSAPRIRIETMVVSSLLSAVMVA
                     L"
     gene            complement(835..>1898)
                     /locus_tag="P5201_10815"
     CDS             complement(835..>1898)
                     /locus_tag="P5201_10815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009164276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="iron ABC transporter substrate-binding protein"
                     /protein_id="HRV99076.1"
                     /translation="PASAAEMTVTDVLGRSVTVPASPERIIASGSGALRLIVYLQAQD
                     RVVAVDSAERRVPNLAVLVSARPYSIANPQFQDMPVFGEFRGMDNPELIAGLSPQPQV
                     IFKVSPLSGPHPDQLTAKTGIPVVGLEYGNLTDKKSDFYATLRTMGQILGKSERAEEI
                     VAFFERHLAELSRRTADIPDDKRPACYIGGVASRGAHGFTSTEPGYPPFLYTNAKNVA
                     ALPGEKTSAVAQISKEKLLEWDPEILFVDLSTTTAGEQASSLYQLTHDPALSALSAVR
                     EGNIWGVLPYNSYTLNYGSVLANGYFVGKTLYPDRFKDIDPAATADEIFTFLVGQPVF
                     SRMNEEFSGRAFSKIALGE"
BASE COUNT          349 a          567 c          681 g          301 t
ORIGIN      
        1 agggccacca ttaccgccga gaggagcgag gagactacca tcgtttcgat gcggatgcgc
       61 ggcgcggaga cccccagcga gagggctgtc tcgtcgccgc agtcgatggc gttgaggtcg
      121 aagcggcgca ggaagaagag gcagaaggct cccagcagca gcagccccat cagcggaatg
      181 tcgcgccacg acgcccgcga gacgtcccca aaagtccaga agaccgtcgc ggcgagctga
      241 ctgtcgtccg cgaagtattg caggaacatc gttcccgccg tgaagaggga gctgagggcg
      301 accccggcga ggatcatcac gtccggcgtt ccccgcgtga cccaggtgat gacgagcacc
      361 actcctgtgc agagaagcgc agcgatgaat gccgagccgg tggtgagcca cggattgacg
      421 atggtgacgg cgttgacggc gctgctctgc atgctgcctg ttcccagcac gatgatggag
      481 aatgcagcgc cgaaggccgc cgcgttcgag atgcccagcg tgaagggcga gccgagcggg
      541 ttgcgcagca ccgactgcat cgccgctccg gcgagggaga gcccccctcc cgccagcagc
      601 gcggcgagcg tctgcggaag gcggatgttg agcacgatga tgcgccactt cgcctctccg
      661 ccgccgagaa gcgtgcggac cacgtcgaga ggggggatgg agacggcgcc ccttgatatg
      721 gagaagacaa ccatcagcac gcaggcggcg gccagcccga agaggaagag tcccttgcgc
      781 tgcacgtggt gttcataggc gggagagata tggcgtcctt ccagatgcac gtcgctactc
      841 cccgagtgcg attttcgaga acgcgcgccc cgagaactcc tcgttcatcc tcgaaaagac
      901 aggctgcccc acgagaaagg tgaagatctc gtcggccgtc gccgccgggt cgatgtcctt
      961 gaagcggtcg gggtagagcg tcttcccgac gaaatagccg ttggcgagca ccgagccgta
     1021 gttcagcgtg tacgagttgt agggcagcac gccccagatg tttccctcgc gcacggcgga
     1081 aagcgccgag agcgcggggt cgtgcgtcaa ctggtagagc gagcttgcct gttcgcctgc
     1141 ggtcgtggtg ctcaagtcga cgaagagaat ctccggatcc cattcgagca gcttctcctt
     1201 ggaaatctga gcaacggcgg aggtcttctc gccggggagc gcggcgacgt tcttcgcgtt
     1261 cgtatagagg aagggcgggt atcccggctc cgtcgaggtg aaaccgtgcg cgccgcgcga
     1321 ggccacgccc ccgatgtagc acgcggggcg tttgtcgtcg gggatgtccg ccgtccggcg
     1381 ggagagttcg gcgaggtggc gctcgaagaa ggcgacgatc tcctccgccc gttcgctctt
     1441 gccgaggatc tgtcccatgg tgcggagcgt ggcgtagaag tcgctcttct tgtcggtgag
     1501 attgccgtac tcaagcccca ccaccggaat gccggttttg gccgtgagct ggtcggggtg
     1561 cgggccggag agcggggaga ccttgaagat cacctgcggc tgcggcgaga gcccggcgat
     1621 cagctccgga ttgtccattc cgcggaactc gccgaagacc ggcatgtcct ggaactgcgg
     1681 gttcgcgatg gagtacgggc gggccgagac gaggacggcg aggttgggaa cgcgccgttc
     1741 ggcggagtcg acggcgacca ccctgtcctg cgcctgaagg tagacgatca agcggagcgc
     1801 tccggaaccg gaggcgatga ttcgctcggg cgaggcgggt actgtcacgg agcggccgag
     1861 gacgtcggtc acggtcatct ccgcggcgga cgcgggta
//
LOCUS       DAOTIL010000565         1893 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminobacteriaceae bacterium isolate AMR_MDS_3026
            NODE_72305_length_1893_cov_3.748640, whole genome shotgun sequence.
ACCESSION   DAOTIL010000565 DAOTIL010000000
VERSION     DAOTIL010000565.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868050
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminobacteriaceae bacterium (wastewater metagenome)
  ORGANISM  Aminobacteriaceae bacterium
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae.
REFERENCE   1  (bases 1 to 1893)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1893)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:19:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,224
            CDSs (total)                      :: 3,198
            Genes (coding)                    :: 3,184
            CDSs (with protein)               :: 3,184
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1893
                     /organism="Aminobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_72305_length_1893_cov_3.748640"
                     /isolate="AMR_MDS_3026"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:3034609"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..239)
                     /locus_tag="P5201_10820"
     CDS             complement(<1..239)
                     /locus_tag="P5201_10820"
                     /inference="COORDINATES: protein motif:HMM:TIGR01726.1"
                     /note="The N-terminal region of this protein, as described
                     by TIGR01726, is a three transmembrane segment that
                     identifies a subfamily of ABC transporter permease
                     subunits, which specificities that include histidine,
                     arginine, glutamine, glutamate, L-cystine (sic), the
                     opines (in Agrobacterium) octopine and nopaline, etc;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_process: GO:0015837 - amine transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HRV99077.1"
                     /translation="MEFSVILEKMGVGMGRASLIFLLTLVLAMPLGVLVALGRMSKSK
                     IVSGIVRIYISVMRGTPLMLQLIAVYFGPYYLLGIS"
     gene            complement(406..1620)
                     /locus_tag="P5201_10825"
     CDS             complement(406..1620)
                     /locus_tag="P5201_10825"
                     /inference="COORDINATES: protein motif:HMM:NF033542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS110 family transposase"
                     /protein_id="HRV99078.1"
                     /translation="MEEMYARCCGIDVHKKKFTACYREGGKNEIREYETTSEGIRGFV
                     GWLKERGAQISAMESTGVYWKSLYNIMEENGLEVIVVNAGHMKAVPGRKTDKNDAEWI
                     AELLACGLLKASYIPDREQREMREITRYRKSLAEERARELNRLQKVLEGANIKLSCVV
                     SDINGQSARRIIGALVGEKGNPKREEVVDLLDERMKPKADEIMKAIDGYVTPMQKQLI
                     RAITAHIDDMSKRIKELDKLIDDEMNKHRNNTRRLDEIPGIGKRTAETILSEIGSDMQ
                     RFPTEAHLSSWSGLSPGNNESAGKRHSGKTNKGNRTLKTALIQAARAAVHGDNYFRAQ
                     YERLLVKRGKNRAVVAVAHSMLIVIYHMLKDGTRYQELGSDYYARRDPRKEIARLQKK
                     MDYLNTLIQTPV"
BASE COUNT          337 a          623 c          437 g          496 t
ORIGIN      
        1 gagatcccga ggaggtagta cggcccgaag tacaccgcga ttaattgaag catcagcggc
       61 gtcccgcgca tgacggatat gtagatgcgg acgatccccg atacgatttt gctttttgac
      121 atccgcccca gcgccaccag cacgcccagc ggcatggcca aaacgagcgt caaaaggaag
      181 atcaggctcg cgcgccccat gcccacgccc atcttctcaa ggatcacgga aaactccatt
      241 caaccacctc gccatcgcaa actcggtcgc ccggggtttg cccgcgagcg accgaagaca
      301 aaatcacggt tctgtccacc cagaatggga ttgctatgaa actctccgca ctttaaggca
      361 taagaatgca cggctccctt atcagggggc cattcagagg gaatatcaga cgggagtctg
      421 aatgagggtg ttcaggtagt ccatcttttt ctggagtcgt gcgatttcct ttcgcgggtc
      481 gcgccttgcg tagtagtcgc tccccagttc ctggtatcgc gtgccatcct tgagcatgtg
      541 gtagatcaca atcagcatag agtgcgcaac agcgaccacc gcgcggtttt tcccgcgctt
      601 cacaagcagc cgttcatact gagcacggaa gtagttgtcc ccatgtactg cggctctcgc
      661 agcttggatc agcgcggtct tcagcgttcg gttccctttg tttgtcttcc cactgtgccg
      721 ctttcccgcg ctttcgttat tcccggggct cagtccgctc cacgatgaca ggtgcgcctc
      781 tgtcggaaat ctttgcatat cactgccgat ctctgacaga atcgtctcgg ccgtccgctt
      841 cccgattccc ggaatttcat ccagccgacg cgtattgttt ctgtgcttgt tcatctcatc
      901 gtcgatgagc ttatccaact ccttgatccg tttgctcatg tcgtctatgt gcgcggtgat
      961 cgcgcggatc agttgcttct gcatcggcgt cacgtatccg tctatcgcct tcatgatctc
     1021 gtccgccttc ggcttcatcc gctcatccag aaggtccacg acctcctcgc gcttggggtt
     1081 ccctttttct cccacaagcg ctccgatgat ccgtcgcgcg ctctggccgt tgatgtcgct
     1141 taccacacaa ctcaacttga tgttcgcgcc ttccagtacc ttctgcagcc ggttcagttc
     1201 ccgcgctctc tcctccgcca ggctcttgcg ataccgcgtg atctcccgca tctcccgttg
     1261 ctcccgatcc ggtatgtagc tcgccttcaa cagtccacat gccaacagct ccgcgatcca
     1321 ctccgcatcg ttcttatctg tcttgcgacc cggtaccgcc ttcatgtgcc ccgcgttcac
     1381 gacgatgact tccagaccgt tctcttccat gatgttgtac agggacttcc agtatacccc
     1441 cgtgctctcc atggcagaaa tctgcgcgcc acgctccttc agccacccta cgaatcctcg
     1501 aatcccttcg gatgtcgttt catattcccg tatctcgttt tttcctccct cccgatagca
     1561 tgccgtaaac ttcttcttat gcacgtcgat tccacaacac ctcgcgtaca tctcctccat
     1621 ggctctttcc ccttccttgg agccgggcgt acctatcttt gttagattgc tatgcatctt
     1681 cgtatgatat cttgtggttc aaccaggtac acccactcat attctcaatc gtcctctcac
     1741 ggacaaatcc cagcgagctt gcgctctact gggattatac cgcttctatt ctgtttcgta
     1801 catcgtggtg taccctggtt cgtgactatt ctcaagggac aacgtccctt gagcgggggt
     1861 ccgggggcag agcccccggc gtcccccgcg cct
//
LOCUS       DAOTIL010000566         1892 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminobacteriaceae bacterium isolate AMR_MDS_3026
            NODE_72363_length_1892_cov_3.545999, whole genome shotgun sequence.
ACCESSION   DAOTIL010000566 DAOTIL010000000
VERSION     DAOTIL010000566.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868050
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminobacteriaceae bacterium (wastewater metagenome)
  ORGANISM  Aminobacteriaceae bacterium
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae.
REFERENCE   1  (bases 1 to 1892)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1892)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:19:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,224
            CDSs (total)                      :: 3,198
            Genes (coding)                    :: 3,184
            CDSs (with protein)               :: 3,184
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1892
                     /organism="Aminobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_72363_length_1892_cov_3.545999"
                     /isolate="AMR_MDS_3026"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:3034609"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..283)
                     /locus_tag="P5201_10830"
     CDS             complement(<1..283)
                     /locus_tag="P5201_10830"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRV99079.1"
                     /translation="MQSQSSIIDRVFLVLLLIIVAHASPSEAASARHIVLLPTLNYTE
                     YEIWESKYYPVNVLEQKMTEYLASLLRQNPFTDVSILDDSQAQRWMDDPY"
     gene            531..893
                     /locus_tag="P5201_10835"
     CDS             531..893
                     /locus_tag="P5201_10835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014162219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004784 - superoxide dismutase activity
                     [Evidence IEA];
                     GO_process: GO:0019430 - removal of superoxide radicals
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="desulfoferrodoxin"
                     /protein_id="HRV99080.1"
                     /translation="MKKLDIFKCELCGNVVELLHVGGGDLVCCGQPMKLLDEQSADSA
                     TEKHVPVIEGNKVKVGSVPHPMTEQHYIEFIEIMAGDKLCRKFLNPGEAAEAVFEGFV
                     PEKSREYCNIHGLWKAEK"
     gene            896..1390
                     /locus_tag="P5201_10840"
     CDS             896..1390
                     /locus_tag="P5201_10840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005661266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferritin"
                     /protein_id="HRV99081.1"
                     /translation="MISKKIEDAINDQINAELYSAYIYLSMSSWFDSANLKGMSHWMG
                     IQAKEEVEHAMKFAGYVMERGGRVLYKAVEGPETEWTSPLEAFKVAYEHEKYVTRRIN
                     DLMDLAVAEKDYASQALLQWFVTEQVEEEANADEIVSKLEMIGEGKHGLLMIDRELGG
                     REDD"
     gene            1484..>1892
                     /locus_tag="P5201_10845"
     CDS             1484..>1892
                     /locus_tag="P5201_10845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013047810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavin reductase family protein"
                     /protein_id="HRV99082.1"
                     /translation="MENGIDPRALFTLNYGVYILSTGYEGKYNGQIINALMQLTADPI
                     CMAACLHKDNYTTELVEKSGRFSVSVLEESVPLKFIGVFGFRCGRDFDKFGECRYEVK
                     EGGLPLVTEYSLAGIELKVLSVQDVFTHKVFIGQ"
BASE COUNT          523 a          398 c          528 g          443 t
ORIGIN      
        1 agtaaggatc atccatccag cgttgcgcct gcgagtcatc cagtatcgaa acatcagtaa
       61 aggggttctg ccgaaggaga gaggccagat actccgtcat cttctgttca agcacattca
      121 ctggatagta cttactctcc caaatttcgt actccgtgta attcaacgtg ggaagcaaca
      181 cgatatgacg tgccgaagcg gcttcggatg gagatgcgtg cgccacgata atcagcagca
      241 aaaccaagaa aacccggtct attattgatg attgagactg cattggttct catcctcttt
      301 tcaaatggag tccaggctgc tcttaataat gtatcggcag aagatactac cggtaaaggg
      361 acagccaagg aaggaggcat tcgtagaaat gcgctctccg tcttcttccc gcttcctatt
      421 atagagcata ttctgattca agggtggaga ggatgagaat tgacttggaa ggtgatataa
      481 taaatctgtt ttcaagaaaa aaaacgattt caaaggagga aagatgctga atgaaaaagc
      541 tggatatctt taagtgtgag ctgtgcggca atgtcgtgga actgcttcac gtcggagggg
      601 gcgaccttgt ctgttgcggt cagcccatga agctccttga tgagcaatcg gcggattctg
      661 caacggagaa acacgtgccg gtcatcgaag gtaacaaggt gaaggttggc agcgttcctc
      721 atccaatgac tgagcagcac tatatcgagt tcattgaaat tatggcagga gacaagctat
      781 gcagaaagtt cctgaacccg ggtgaagccg cagaggctgt ttttgaggga tttgttcctg
      841 agaagagcag ggaatactgc aatatacatg gtttatggaa ggcggaaaag taagcatgat
      901 cagtaaaaaa attgaagacg cgataaacga tcagatcaat gcggaacttt actctgcgta
      961 tatctacctt tcgatgtcgt cttggttcga ttcggcgaat ttgaagggaa tgtctcactg
     1021 gatgggaatc caggcgaagg aagaagtaga gcatgcgatg aaattcgccg gctatgtaat
     1081 ggagcgcggc ggaagagtcc tgtataaagc tgtggaggga ccggaaaccg agtggacttc
     1141 tcctttggaa gcctttaaag ttgcgtacga acatgagaag tatgtgactc gccggatcaa
     1201 cgacctcatg gatctcgccg tggccgagaa ggattatgca agccaggcgc ttctccaatg
     1261 gtttgtcacg gagcaggtcg aagaagaggc gaatgcggac gaaatcgtga gcaagcttga
     1321 gatgattggc gaagggaagc acggcctcct gatgatcgac agagaattgg gtggcagaga
     1381 ggacgactag aagtccttga agcatctcct tagtgaagac aatctgcggg caggagcttc
     1441 aggctcctgc ccgcagtgca ggaagatgaa gggaggaaac tcaatggaaa acgggatcga
     1501 cccccgggct ctttttacat tgaattatgg agtgtacatt ctcagtaccg ggtatgaagg
     1561 caaatacaac ggacagatca tcaacgcgct tatgcagctt accgctgacc cgatctgcat
     1621 ggcagcttgc cttcacaagg ataactacac aacggagctc gtggagaaga gcgggcgttt
     1681 ctccgtctcc gtgctcgaag aatcagtccc tttgaagttc atcggcgtct tcggtttccg
     1741 ttgcggacgc gatttcgata aattcgggga atgcaggtac gaagtgaaag agggcggtct
     1801 tcctcttgta acggagtatt ctctggcggg catagaactg aaagttctct ctgtacaaga
     1861 tgtcttcaca cataaggtct tcatcggcca gg
//
LOCUS       DAOTIL010000567         1889 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminobacteriaceae bacterium isolate AMR_MDS_3026
            NODE_72519_length_1889_cov_3.402944, whole genome shotgun sequence.
ACCESSION   DAOTIL010000567 DAOTIL010000000
VERSION     DAOTIL010000567.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868050
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminobacteriaceae bacterium (wastewater metagenome)
  ORGANISM  Aminobacteriaceae bacterium
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae.
REFERENCE   1  (bases 1 to 1889)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1889)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:19:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,224
            CDSs (total)                      :: 3,198
            Genes (coding)                    :: 3,184
            CDSs (with protein)               :: 3,184
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1889
                     /organism="Aminobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_72519_length_1889_cov_3.402944"
                     /isolate="AMR_MDS_3026"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:3034609"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1137)
                     /locus_tag="P5201_10850"
     CDS             complement(<1..1137)
                     /locus_tag="P5201_10850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013047831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside kinase"
                     /protein_id="HRV99083.1"
                     /translation="MAFTIEVANGPVFSSDVPVTGKSVLSESRPDGEEKTIVAWRVNN
                     YLRSLDWVIEDNARVEFIDTSSFEGMEVYRRSLSFLLVLACKRGLAKDVVLRHSISEG
                     YYWEFVEGEVTDEDLAVIKATMADLVGKDLPFIRKVVPLDKARRIFDRQGSGEVARLF
                     QWGAVDPVELYRCADMYGYYYAPLAPSTGYLKLYDLKKLGPGMALQFPTISYPSSLPP
                     FRASKNLSGVFLDYAKWLEVLDLRTMDSLHKKVAEGRSQEVILIAEAFHAQKLGRIAD
                     EIASKTKVKVAAIAGPSGSGKTTFSERLKIQLLVCGRNPVTLAMDNYFVNREDTPRDE
                     QGNLDFEVVEALELELLEDHLRRLLAGEEVVTPQFSFIEGKKKPG"
     gene            1231..>1889
                     /locus_tag="P5201_10855"
     CDS             1231..>1889
                     /locus_tag="P5201_10855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006301532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FadR/GntR family transcriptional regulator"
                     /protein_id="HRV99084.1"
                     /translation="MENVKGKTKIYDQVVDSIKLRISEGELPQGSALPPERQLIDELN
                     VSRSSLREGFRILEMMGLIESIPGKGRFVRTSRSDGGATGRVPLQDEAILELVEARLV
                     LEPAIAVEAAKHASPSDLTRIRRILTKTGKDVESLEHRAQCDFDFHLVLAESTHNFIF
                     VNIVKMTFNLIMATHERIYTLLDDKEIFLREHEEVYDAIVGRDIDRAANLMVDHVRRV
                     YR"
BASE COUNT          468 a          495 c          480 g          446 t
ORIGIN      
        1 accgggtttt ttctttccct cgataaaact aaactgaggg gtgacaacct cttctccggc
       61 gagaaggcgg cgaaggtggt cctccagaag ttccaattcg agcgcctcca ctacttcaaa
      121 atcgaggttg ccttgctcgt cgcgcggagt atcctcccgg ttcacaaagt agttatccat
      181 agcaagcgtc acgggatttc tgccgcagac gagaagctgt attttcagcc tttcggaaaa
      241 agttgtcttt ccggagcctg aaggccctgc aatcgccgct accttcactt ttgtctttga
      301 ggctatttca tcggcgatcc gccccaattt ctgcgcatgg aatgcctctg cgataaggat
      361 aacttcttga cttctgccct ccgcaacctt tttatgcaga ctgtccattg tccggagatc
      421 aagcacttcc agccatttag catagtccag gaaaactccg gagagatttt tcgaagcgcg
      481 gaatggaggc agagaggaag gatagctgat tgtcgggaat tgaagcgcca tcccgggtcc
      541 gagctttttg agatcgtaga gcttcaaata cccggtcgac ggggcgagcg gggcatagta
      601 gtatccgtac atatctgcgc aacgatagag ttccacagga tcgacggcgc cccactgaaa
      661 gagccgtgca acttccccgc ttccctgccg gtcgaaaatt cttctggcct tatcgagggg
      721 gacaactttt cgaataaacg gcagatcctt tccgacaaga tcagccattg tggccttgat
      781 gaccgcaagg tcttcgtccg tgacttctcc ctctacaaac tcccagtagt acccttcact
      841 gatggagtgg cggagaacga cgtctttcgc caagcctcgt ttacatgcaa gcacgaggag
      901 gaagctcagg gagcggcgat acacttccat gccttcaaag gaggatgtat caatgaactc
      961 aacccgtgca ttgtcttcaa taacccagtc gagtgaacga aggtaattgt ttactctcca
     1021 cgcgacgatg gtcttctctt cgccgtctgg gcggctctcc gaaagaacgg attttcctgt
     1081 gaccggaacg tcgctggaaa aaacaggccc gtttgcaact tcaatagtga atgccataga
     1141 acgttctcct cccttcgata aacgggttgc gctgtatttg aaagtagatt atgcttatat
     1201 taccatacgg aacacaggaa ggagacacac atggaaaacg taaaagggaa gacgaaaata
     1261 tacgaccagg tggtcgatag tattaaactg cgtatttccg agggagaact gccccaaggc
     1321 tccgcgcttc cgccggaacg ccagcttata gacgaactga acgtcagccg cagctcgctt
     1381 cgggaaggat ttcgcattct ggaaatgatg ggtttgatcg aaagtattcc gggcaaaggc
     1441 cgttttgtga ggacgtcgcg aagcgacggc ggagcgaccg gccgagtccc gctccaggac
     1501 gaggctatcc tggaactcgt cgaagcgcgg cttgtgcttg aacctgcaat agccgtcgaa
     1561 gcggcaaagc atgcttctcc ctccgacctc acgagaatac gccgaattct aacaaaaacg
     1621 gggaaagacg tggaatcttt ggagcaccga gcccaatgcg atttcgattt tcatcttgtt
     1681 ctggcggagt caacccataa tttcattttt gtcaacatcg tcaagatgac gttcaacctg
     1741 attatggcga ctcatgaacg tatctatacg ctcctcgacg ataaagagat ttttcttcgt
     1801 gaacacgagg aagtttatga cgccatcgta ggacgcgaca tcgaccgcgc cgcgaacctg
     1861 atggtcgacc atgttcgaag agtctaccg
//
LOCUS       DAOTIL010000568         1887 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminobacteriaceae bacterium isolate AMR_MDS_3026
            NODE_72636_length_1887_cov_3.409389, whole genome shotgun sequence.
ACCESSION   DAOTIL010000568 DAOTIL010000000
VERSION     DAOTIL010000568.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868050
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminobacteriaceae bacterium (wastewater metagenome)
  ORGANISM  Aminobacteriaceae bacterium
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae.
REFERENCE   1  (bases 1 to 1887)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1887)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:19:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,224
            CDSs (total)                      :: 3,198
            Genes (coding)                    :: 3,184
            CDSs (with protein)               :: 3,184
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1887
                     /organism="Aminobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_72636_length_1887_cov_3.409389"
                     /isolate="AMR_MDS_3026"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:3034609"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(75..449)
                     /locus_tag="P5201_10860"
     CDS             complement(75..449)
                     /locus_tag="P5201_10860"
                     /inference="COORDINATES: protein motif:HMM:TIGR00004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019239 - deaminase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rid family detoxifying hydrolase"
                     /protein_id="HRV99085.1"
                     /translation="MKKVIRTDKAPDPIGPYSQGLVCGNRVYVSGQGPRNPETGKNPE
                     GIKEQTRQVLKNIKAILEAGGATMDQVVKVTAHLTNLDRDFQGYNEVYKEFFTEPYPV
                     RITVQSQLFGMLVEIDAIAELD"
     gene            594..1250
                     /gene="eda"
                     /locus_tag="P5201_10865"
     CDS             594..1250
                     /gene="eda"
                     /locus_tag="P5201_10865"
                     /EC_number="4.1.2.14"
                     /EC_number="4.1.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013049050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008675 -
                     2-dehydro-3-deoxy-phosphogluconate aldolase activity
                     [Evidence IEA];
                     GO_function: GO:0008700 - 4-hydroxy-2-oxoglutarate
                     aldolase activity [Evidence IEA];
                     GO_process: GO:0009084 - glutamine family amino acid
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0009255 - Entner-Doudoroff pathway through
                     6-phosphogluconate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 4-hydroxy-2-oxoglutarate
                     aldolase/2-dehydro-3-deoxy-phosphogluconate aldolase"
                     /protein_id="HRV99086.1"
                     /translation="MNIRKFEVLRRISDLGVVGIIRTPDIEKGVAAAEAVFEGGIDVL
                     EVSTTFPGALDIMKEIIERGRCKGLILGAGTVADATTARLCIDAGAEFIVSHCFSEEV
                     ARMCNRSGIAYMPGVGTVTEVVRAMEHGVDVVKAFPGEVLGPKFIKAVHGPLPGAQIM
                     PIGGVAPGNLDDWFQAGAFAVGLGSALLKPAGTEGDFQAISETAERIVTQIAQIRAHL
                     "
     gene            1281..>1887
                     /locus_tag="P5201_10870"
     CDS             1281..>1887
                     /locus_tag="P5201_10870"
                     /inference="COORDINATES: protein motif:HMM:NF013088.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HRV99087.1"
                     /translation="MSSHWIGVFAALVTGVAWGVVSPLGRMLALRGADMTTVVVVRTL
                     LAACILFLWLLLKNPRSLRISFKDGLVLLAYAVLAIPLTATGFLYSLKYLTVPAALII
                     HYTFPLATLLGGLWITGERPTAPQYIAALLVIAGVWLGVFSSGTADGSFSLLGVFWGL
                     LAVVGLSGQFLMGRVLLSGKRLSGVLLIFYSHLFGGLIMAAL"
BASE COUNT          320 a          583 c          575 g          409 t
ORIGIN      
        1 gctgcgaggg ggcgtttatg ttgcgtttta ctaagcatcc ctgtacgcga gcgcaggtat
       61 ccgatccgcc gttttcagtc caactcggcg atggcgtcga tctccaccag cataccgaag
      121 agctggctct gcacggtgat ccgcacaggg tacggctcgg tgaagaactc cttgtacacc
      181 tcgttgtacc cctggaaatc gcggtcgagg ttcgtcaggt gcgccgtcac cttgaccacc
      241 tggtccatcg tcgcgccgcc cgcctccagg attgctttga tgttcttaag cacttgccgc
      301 gtctgttcct taattccttc tggattcttc cccgtctcgg gatttctagg gccctgtccc
      361 gaaacgtaga cgcggttgcc gcagaccagc ccctgggagt acggcccgat gggatccggc
      421 gctttgtcag tacggataac ctttttcact gcaatcatcc tctcgtaagt ttttctcccc
      481 cccttccgcg aaaaaaacgc cggacatgca aggaaagaga ctttctttgc tatcattgta
      541 tccgaaaaag ctctcgaacg ggaagccata ctcttgtcag gaggatgcaa agaatgaata
      601 tccgaaagtt tgaagtgctg cggcggatct ccgacctcgg cgtggtaggg atcatcagga
      661 ctccggacat cgagaagggc gtggcggcgg cggaggcggt tttcgaagga ggcatcgacg
      721 tgctcgaagt ctccacgacc ttcccgggcg ccctcgacat catgaaggag atcatcgaac
      781 gcggccgctg caaagggttg atactcggcg cgggaacagt cgccgacgca acgacggcgc
      841 gcctctgcat cgacgcgggg gcggagttca tcgtctctca ctgcttcagc gaggaggttg
      901 cgcggatgtg caacaggtcc ggcatcgcct acatgcccgg cgtggggacg gtcaccgagg
      961 tcgtccgcgc gatggagcac ggcgtcgacg tcgtgaaggc gtttccggga gaagtgctgg
     1021 ggccgaagtt catcaaggcg gtccacggtc ccctgcccgg cgcgcagatc atgccgatcg
     1081 gcggcgtggc gccgggcaat ctcgacgact ggttccaggc gggcgccttc gccgtagggc
     1141 tcggaagcgc gctcctgaaa ccggcgggaa cggaaggaga cttccaagcc atcagcgaga
     1201 cagccgaacg gatcgtcacc cagattgccc agatccgcgc tcatctataa ggcgaagtcc
     1261 gccgcaggag ggaagagagg atgtcatcac actggatagg cgttttcgcg gcgctggtca
     1321 ccggcgtcgc atggggggtc gtcagcccgc tcggccgcat gctcgccctt cggggcgccg
     1381 acatgacgac cgtggtcgtc gtacggacgc ttctggcggc atgcattctc tttctctggc
     1441 tcctgctgaa aaatccccgg tctctccgca tttctttcaa ggacggattg gtgcttcttg
     1501 cgtacgccgt cctcgccatt ccactcacgg cgaccggatt tttgtattct ctgaagtacc
     1561 ttacggttcc ggcggcgctc atcatccact acacattccc cctcgccacg ctgctcggag
     1621 ggctctggat caccggggaa cggccgaccg ccccgcagta catcgccgcg ctccttgtca
     1681 tcgcgggcgt ctggctcggc gtcttctcat ccggtacggc cgacgggagc ttttcgcttc
     1741 tcggggtctt ctgggggttg ctcgcggtcg tcgggctttc gggccagttt ctgatggggc
     1801 gggtgttgct ctcgggcaaa cgcctctccg gtgtgctcct gatcttctac tcccacctgt
     1861 tcggcggtct gatcatggcc gcgctca
//
LOCUS       DAOTIL010000569         1887 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminobacteriaceae bacterium isolate AMR_MDS_3026
            NODE_72641_length_1887_cov_3.193777, whole genome shotgun sequence.
ACCESSION   DAOTIL010000569 DAOTIL010000000
VERSION     DAOTIL010000569.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868050
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminobacteriaceae bacterium (wastewater metagenome)
  ORGANISM  Aminobacteriaceae bacterium
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae.
REFERENCE   1  (bases 1 to 1887)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1887)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:19:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,224
            CDSs (total)                      :: 3,198
            Genes (coding)                    :: 3,184
            CDSs (with protein)               :: 3,184
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1887
                     /organism="Aminobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_72641_length_1887_cov_3.193777"
                     /isolate="AMR_MDS_3026"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:3034609"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..260)
                     /locus_tag="P5201_10875"
     CDS             complement(<1..260)
                     /locus_tag="P5201_10875"
                     /inference="COORDINATES: protein motif:HMM:NF013069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HRV99088.1"
                     /translation="MGIIGISIRRPVLTLVIVILAVLLGLSGMQGMGISLLPRIEVPF
                     VSVVTRYTGAGPEDMETLVTKPLEDAISQVQGAGGLERPRFEG"
     gene            complement(261..1370)
                     /locus_tag="P5201_10880"
     CDS             complement(261..1370)
                     /locus_tag="P5201_10880"
                     /inference="COORDINATES: protein motif:HMM:TIGR01730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HRV99089.1"
                     /translation="MLFWILLLAGALGFLGYRVVAPSGAQQRIPPPSAGIMVTLYEVQ
                     PRLWEIWRSFFGTVRSAQVQKVTSQTREVIEEINVEIGDSVKKGDVLISLAGQSQVAL
                     LSARQAAYDDAKSRYDRLQSLFQAGGTSKQELDKANVSVQEEAAKLRDARTTVSRIQI
                     RSALNGIVVRRNAERGEFADPGRELLAIADFDRREVEMLISPTIRGLIRPGMDVQVKS
                     PWGGQTMGKIFRMDPEADSATGFFRGIVRLPPECTLIPGDYVRMEVRMVHKENTLAVP
                     YECILREDGQPYVFIVDGEKAVRRNILAGEGSGGYVEISSGLAPGDKVVKIGAGSLYE
                     GAKVLVSNGLLRTAAGSGVKKTEPGTASADISGEK"
     gene            complement(1538..>1887)
                     /locus_tag="P5201_10885"
     CDS             complement(1538..>1887)
                     /locus_tag="P5201_10885"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRV99090.1"
                     /translation="RERVAPHMGEDSGVKGTVLSVVLGACSAGPLYIAFPVAEVMIRK
                     GTSLRNIFVFIGAWSTMRIPMILFEIQNLGAVFGLTRYFVSLVGILLMALLMDRVLNT
                     AEKTEIIARFQEE"
BASE COUNT          414 a          595 c          475 g          403 t
ORIGIN      
        1 ccttcaaagc gaggtctttc taatcctccg gccccctgca cctggctgat ggcgtcctcc
       61 agcggcttag taacgagcgt ctccatatcc tccgggccgg ccccggtgta ccgagtgact
      121 acggagacga acggcacttc gattcgcgga agaagcgaga tccccattcc ctgcataccc
      181 gaaagtccca gaagcactgc gagaatcacg atgaccaggg tcaggaccgg ccgcctgatc
      241 gaaattccga tgatacccat ctatttctct ccggaaatgt cggcgctcgc tgtccctggt
      301 tccgtctttt ttactccgga acctgcggct gttcgcaata aaccgttcga gacgaggacc
      361 ttcgccccct cgtagagact tccggcgcct attttcacaa ccttgtcgcc cggggcgagg
      421 ccagaggaaa tttcgacata gcctccgctc ccctctccag cgaggatatt ccggcgaacc
      481 gccttttcgc cgtcgacgat aaagacatac ggctgcccat cctcgcggag aatgcattca
      541 taggggacgg caagcgtatt ctctttgtgc accatcctga cttccattct gacgtaatcg
      601 cctgggatga gagtacactc cggcggaaga cgaacaatcc ccctgaagaa tcccgtcgcc
      661 gaatccgcct cgggatccat ccgaaagatt ttccccatgg tctgtccgcc ccagggagac
      721 ttcacctgca cgtccatacc cgggcgaatg agccctcgta tggtgggaga gatcagcatc
      781 tccacctccc ggcggtcgaa gtcggcgatc gccaacagct cgcgccccgg gtccgcgaat
      841 tccccccgct cggcattcct ccgaacgacg attccgttga gagctgaacg gatttgaata
      901 cgggagacag tcgtcctggc gtcccgaagc ttggcggctt cttcctggac ggagacgttc
      961 gccttgtcca gttcctgctt tgatgttccg cccgcctgaa agagactctg gagacgatca
     1021 taacgggatt tagcatcgtc ataggctgcc tgacgagcgc tcaaaagcgc cacctggctc
     1081 tgtccggcaa gggagatgag cacatctccc ttcttcacgc tgtcgcctat ctcgacgttg
     1141 atctcctcaa taacctcgcg cgtctgagat gtcaccttct gtacctgcgc ggagcgcacc
     1201 gtcccgaaaa agctgcgcca gatttcccag agacgcggct ggacttcgta aagggtcacc
     1261 atgattccag cggaaggggg cggtattcgc tgctgcgccc cggaaggcgc aacaacccta
     1321 taccctagga aaccgagggc tccggcaaga agcagaatcc agaacaacac atttgctttc
     1381 ctcaataaaa ccatggcgtc ccccttgcgg ttgcaaagcg ataacgaatc tttgctctgt
     1441 ctaccctttc ccataatatc tttattgtac atccggagct ttctttttcg ctatgcgcaa
     1501 ttacgtaaaa ctcgttccct ataaggaacg aaagcgctta ctcctcctga aaacgggcta
     1561 tgatctccgt cttctctgcc gtgttgagca ctctgtccat aagaagcgcc atgagaagga
     1621 tgccgacaag cgagacgaaa taccgggtga gtccgaagac cgccccaaga ttctggatct
     1681 cgaaaagaat catcgggatg cgcatcgtag accatgcgcc gatgaaaacg aagatgtttc
     1741 gcaggctcgt tcctttacga atcatcacct cggcgacggg aaaggcaata tagagaggcc
     1801 ctgcggagca tgccccaagg actacggaga gcacagtccc cttcacgccg gagtcttcgc
     1861 ccatatgcgg cgctaccctc tccctcg
//
LOCUS       DAOTIL010000570         1883 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminobacteriaceae bacterium isolate AMR_MDS_3026
            NODE_72845_length_1883_cov_2.131291, whole genome shotgun sequence.
ACCESSION   DAOTIL010000570 DAOTIL010000000
VERSION     DAOTIL010000570.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868050
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminobacteriaceae bacterium (wastewater metagenome)
  ORGANISM  Aminobacteriaceae bacterium
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae.
REFERENCE   1  (bases 1 to 1883)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1883)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:19:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,224
            CDSs (total)                      :: 3,198
            Genes (coding)                    :: 3,184
            CDSs (with protein)               :: 3,184
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1883
                     /organism="Aminobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_72845_length_1883_cov_2.131291"
                     /isolate="AMR_MDS_3026"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:3034609"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..515)
                     /locus_tag="P5201_10890"
     CDS             complement(<1..515)
                     /locus_tag="P5201_10890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013049264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HRV99091.1"
                     /translation="MYLRLERLDKKFEATEAVRELSLDIERGEMLSLLGPSGCGKTTT
                     LRMVGGFLRPDGGRIVLDGEDITALPPERRPTATVFQSYALFPHMSVLRNVIYGLKFR
                     KVPREEALSAGRAMLEKVGLPGAGGKTIDQLSGGEQQRVALARALIVRPKVLLLDEPL
                     SNLDAKLRVRMR"
     gene            complement(487..>1883)
                     /locus_tag="P5201_10895"
     CDS             complement(487..>1883)
                     /locus_tag="P5201_10895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013049263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006826 - iron ion transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HRV99092.1"
                     /translation="FRGKRAVLGVLLLTLISPPFVSSLSYIMLFGRRGLVTWRLLGLR
                     WNPYGAHGVVLMESVGLAAVAAFLIMAVLRGIDRSLEQASFDLGAGRWETLLRVTLPL
                     ARPGVVTAALIVFIRSLSDFGTPMFIGGNYNVLATQAYMTAMGSFDLPKAAAISTLLV
                     IPAFLVFLVYRELMAGAPLFSSKAVAAGEERPLPGAVRWVVFAVVWSFVLFEAMKYGA
                     IFCGAFVRTWGVDFSFTLHHLKTLQLTKLDSLLRSVRYAFAAALIAGAMGVVLARFLG
                     RRATLFSRAVDFIADLPFILPGPFFGIAYLLAFNRLPEAFLGTGFLIVANCVYRQLSL
                     GIKSGVSVLGQINPELEDAVRDQGGGSLAVLRDVIGPLLMPAFLVSFINTFTFTMTTI
                     GGIIFLITPYTKVLTAEMFDAIQSGDIGASSVMASVIILVAMTVNVTFSWLFLKRRTK
                     GSEAEYVSSVGAAR"
BASE COUNT          382 a          614 c          608 g          279 t
ORIGIN      
        1 cgcatccgca cgcggagctt cgcgtccagg ttgctcaacg gctcgtcgag gagcagcacc
       61 ttcggccgga cgatcaacgc ccgcgcgagg gctacccgct gctgttcgcc gccgctgagc
      121 tggtcgatcg tcttgcctcc ggcccccgga aggccgacct tctcaagcat cgccctcccc
      181 gccgagaggg cctcttcgcg gggaactttg cggaatttca ggccgtagat cacgttccgc
      241 agcacgctca tgtgcgggaa gagcgcgtag ctctggaaga cggtcgccgt agggcgccgc
      301 tccggcggaa gcgcggtgat atcctccccg tcgaggacga tccgcccgcc gtcgggccgg
      361 agaaagcctc cgaccatccg cagcgtcgtt gtcttgccgc atccgctcgg ccccaaaagc
      421 gagagcatct ccccgcgttc aatatcgaga gagagctccc gtaccgcctc cgtagcttcg
      481 aattttttat cgagccgctc caaccgaaga tacatactcc gcctccgatc cttttgttct
      541 cctcttcagg aagagccacg aaaacgtcac gttcaccgtc atggcgacca ggatgatcac
      601 cgacgccatc accgaactcg cgccgatatc gccgctctgg atagcgtcga acatctccgc
      661 ggtgagcacc ttcgtgtacg gcgtgatgag gaagatgatg ccgccgatgg tggtcatggt
      721 gaacgtgaag gtgttgatga agctcaccag gaacgccggc atgagcagcg gcccgatcac
      781 gtcccgcagc acggcgaggc ttcctccccc ctggtcgcgg acggcgtcct cgagctccgg
      841 gttgatctgc cccagcacgg agacgccgct cttgatcccg agtgaaagct gccggtagac
      901 gcagttcgcg acgatgagaa accccgtccc caggaacgcc tcgggcagcc ggttgaacgc
      961 cagaaggtag gcgatgccga agaacggccc cggcagaatg aaggggagat ccgcgatgaa
     1021 atcgacggcc cggctgaaca gcgtggcgcg cctcccgaga aagcgcgcca gtacgacgcc
     1081 catcgcgccc gcgatcaggg cggcggcgaa ggcgtagcgg acgctccgga gcaggctgtc
     1141 gagcttggtg agctggagcg tcttcaaatg atggagcgta aaggaaaaat cgacgcccca
     1201 cgtcctgacg aaggcgccgc agaagatcgc gccgtacttc atggcctcga agaggacgaa
     1261 gctccagacg accgcgaaga cgacccatcg caccgctccg ggaagcgggc gttcctctcc
     1321 ggcggcgacc gccttggaag aaaagagggg cgccccggcc atcagctctc ggtagaccag
     1381 aaagacgaga aaggctggga tgacgagaag cgtggagatg gcggcggcct tcggaaggtc
     1441 gaaggagccc atcgcggtca tgtacgcctg cgtcgcgagc acgttgtagt tgccgccgat
     1501 gaacatcggc gtgccgaagt cggagagcga gcgaatgaag acgatcagcg ccgccgtgac
     1561 gacgcccggg cgggcgagcg gaagcgtgac gcggagcagc gtctcccacc ttcccgcgcc
     1621 gaggtcgaac gaggcctgtt caagacttct gtcgatgccc cggagcaccg ccattatcag
     1681 gaacgcggcg acggcggcca gcccgacgga ctccatcaga acgacgccgt gcgctccgta
     1741 cgggttccag cgaagcccca gaagccgcca ggtcacgagc cctctccgtc cgaagagcat
     1801 gatgtacgag agggaggaga cgaagggcgg ggagatcagc gtcagcagga ggacccccag
     1861 cacagctcgc ttcccccgga agg
//
LOCUS       DAOTIL010000571         1880 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminobacteriaceae bacterium isolate AMR_MDS_3026
            NODE_72985_length_1880_cov_2.821370, whole genome shotgun sequence.
ACCESSION   DAOTIL010000571 DAOTIL010000000
VERSION     DAOTIL010000571.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868050
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminobacteriaceae bacterium (wastewater metagenome)
  ORGANISM  Aminobacteriaceae bacterium
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae.
REFERENCE   1  (bases 1 to 1880)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1880)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:19:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,224
            CDSs (total)                      :: 3,198
            Genes (coding)                    :: 3,184
            CDSs (with protein)               :: 3,184
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1880
                     /organism="Aminobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_72985_length_1880_cov_2.821370"
                     /isolate="AMR_MDS_3026"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:3034609"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(120..1013)
                     /locus_tag="P5201_10900"
     CDS             complement(120..1013)
                     /locus_tag="P5201_10900"
                     /inference="COORDINATES: protein motif:HMM:NF025124.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HRV99093.1"
                     /translation="MSVYSDEELERMLDDLESDLVERKESFRGDVPKKARQAVWAFSN
                     DLPFDLHPVPEASLNDLSRVLFESEYLPAAFAPEILEANNRTYEERLASCRMVVSPDN
                     PVPTLVGILALGKRPRAFFPGASVQFLRIDGEEFADPVIDEENVDGPLREMLSKIGQK
                     LSAHNRVFVDIVSSPTEKRIYDYPPPALQQLLYNAVLHRTYEKTNTPVRIYWFNDRIE
                     IISPGGPYGNVTRENFGRPGITDYRNPNLAEAMKVFGFVQAFGRGIAVARRALETNGS
                     SPLEFEVDQSAVKAVVRKRQK"
     gene            1341..>1880
                     /locus_tag="P5201_10905"
     CDS             1341..>1880
                     /locus_tag="P5201_10905"
                     /inference="COORDINATES: protein motif:HMM:NF013980.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF45 domain-containing protein"
                     /protein_id="HRV99094.1"
                     /translation="MSARKPREGSLAVCGREISFTVDYGKRKHLRIVVEAERGVEVRA
                     PRSASFAMVTEAVQRRAGWIAGALERVHNAALLHPKPSYTEGEEHLFLGVLYPLSIRT
                     GRGLGELSGGRLALRVPSVPADGESVKRSLNRWYASQAEGVFAASMARCLPRCAAFVQ
                     CAPPLKARFLRARWGSWSSA"
BASE COUNT          348 a          534 c          594 g          404 t
ORIGIN      
        1 atggaagaga tcgagaagta catcaaaaag aaagcgtaga aaaccgccgc ccccgagtgt
       61 tcccgggccg ggggcggcgg ttttcagaga cgcttcggta agacgcgtat cgggtattct
      121 tatttttggc gctttcgtac aaccgccttg actgcgctct ggtcgacttc gaattcaagc
      181 ggtgaactgc cgtttgtctc cagggctctc cgggcgacgg caattcctct tccgaaagct
      241 tgtacaaatc cgaatacctt catcgcctcg gcaagattcg ggttcctgta gtcggtaatc
      301 cccggcctgc cgaagttctc ccgcgtcacg ttgccgtagg gccctcccgg gctgatgatt
      361 tctatcctgt cgttgaacca gtagattcgg accggcgtgt tcgtcttctc atacgttctg
      421 tggagcaccg cgttgtacag cagctgctgc agcgccggcg gcggatagtc gtagattctc
      481 ttctccgtag gggacgagac gatatccacg aacacccggt tatgcgcgga aagtttctgc
      541 ccgatcttcg aaagcatttc tctgagcggg ccgtcgacgt tttcctcgtc gatcaccgga
      601 tcggcaaact cttcgccgtc tattcgcaga aactgcaccg aggccccggg gaagaaggcg
      661 cgcgggcgct ttcccagggc gagaatgccg acgagcgtgg gaacgggatt gtcgggggag
      721 acgaccatcc ggcagctcgc gagccgctct tcgtacgttc tgttgttcgc ctcaagaatc
      781 tccggcgcga aggccgcggg aagatactcg ctctcgaaga gcactctgga aagatcgttc
      841 agcgaggcct ccggaacagg gtggagatcg aacggcaggt cgttggagaa tgcccagaca
      901 gcctgccgcg cttttttcgg cacatccccg cggaaggatt cctttcgttc aacaagatcg
      961 gattcgaggt cgtcgagcat ccgctcaagc tcctcatccg aatagaccga catcgccgca
     1021 ccacctttca gcgaattcag ggcgatgaaa cgcttcgccg cccgattccg ttttctggaa
     1081 tatctttttc ccgtttcttt ataatagcat gaacgcttcg atgtgcggac acacgatgaa
     1141 gaggcggaaa ggcccatctc gagaagcctt tcggagtctt cggatatcct tgaaagagcc
     1201 gagacccgtt ccgtggtatt atggacgtac ctgcactttg agtatctttc tgcttctgcg
     1261 gaaaattcta caacgccgga cggcaaagga gtaatcgtgt acgctcacta tactacgaac
     1321 ttattgacga tgggcgaaga atgagcgcga ggaagccgcg ggagggctcg ctggcggttt
     1381 gcgggcggga gatttcgttc actgtggatt atgggaagcg caagcatctg cgtattgtcg
     1441 tggaagcgga gcggggggtg gaggtgcgtg ctccccgctc cgcgagcttc gccatggtga
     1501 cggaggcggt gcagcggcgg gccgggtgga tcgccggggc gctcgagcgc gtccataacg
     1561 ccgcgctgct ccacccgaag ccctcgtaca cagaggggga ggagcatctc tttctggggg
     1621 tcctctaccc cctttccata cggacggggc ggggcttggg cgaactctcc ggcgggaggc
     1681 tggcgctgcg ggtaccgtcg gttcctgcgg acggcgagtc ggtgaagcgc tcgctcaaca
     1741 ggtggtacgc gtcgcaggcg gagggcgtct tcgcggcgag catggcgcgc tgcctccccc
     1801 gttgcgcggc gttcgtccag tgcgctcccc cgctgaaggc gaggtttctt cgggcgcgct
     1861 ggggaagctg gtcgagcgcg
//
LOCUS       DAOTIL010000572         1879 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminobacteriaceae bacterium isolate AMR_MDS_3026
            NODE_73044_length_1879_cov_2.728070, whole genome shotgun sequence.
ACCESSION   DAOTIL010000572 DAOTIL010000000
VERSION     DAOTIL010000572.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868050
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminobacteriaceae bacterium (wastewater metagenome)
  ORGANISM  Aminobacteriaceae bacterium
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae.
REFERENCE   1  (bases 1 to 1879)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1879)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:19:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,224
            CDSs (total)                      :: 3,198
            Genes (coding)                    :: 3,184
            CDSs (with protein)               :: 3,184
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1879
                     /organism="Aminobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_73044_length_1879_cov_2.728070"
                     /isolate="AMR_MDS_3026"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:3034609"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            247..1347
                     /gene="upp"
                     /locus_tag="P5201_10910"
     CDS             247..1347
                     /gene="upp"
                     /locus_tag="P5201_10910"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008520964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004845 - uracil phosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uracil phosphoribosyltransferase"
                     /protein_id="HRV99095.1"
                     /translation="MKIAVGSDHAAFALKNYLLEHLRQKSGIESVDMGVDTDAVSCDY
                     PDVAFRVGEAVVSRQADRGLLLCGTGVGMSIAANKIPGVYAAHCHDVETARFSRRHNN
                     ANVLTMGARIISQELAREIIDVWLETDFEGDRHIRRLSKILDYEHKICPSCTFSEKGK
                     TVVFEHPLVQHKVSIIRDKNTSVKEFRELVQEIAGLMVYEITRNLPLVKIEVETPLAK
                     TEAYALEGKKLAIVPVLRAGLGMVDGILQLVPNAKVGHIGLYRDPETLLPVEYYCKLP
                     GDISERDILVLDPMLATGGSAAAAISLVKARGGKKVSLVCIIAAPEGVSKVHAEHPDV
                     DIYAAALDSHLNDHGYIVPGLGDAGDRLFGTK"
     gene            1576..>1879
                     /locus_tag="P5201_10915"
     CDS             1576..>1879
                     /locus_tag="P5201_10915"
                     /inference="COORDINATES: protein motif:HMM:NF013146.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl/decaprenyl-phosphate
                     alpha-N-acetylglucosaminyl 1-phosphate transferase"
                     /protein_id="HRV99096.1"
                     /translation="MLRFMAVGSTLVFLSGYWDDMKTLNPAIRLTFHIVAAGLFLLFL
                     PVSLVHIPVCLLWITGMTNAFNLIDGANGLCLMMFIVASSVFAACGFPFFFLPVASL"
BASE COUNT          404 a          472 c          500 g          503 t
ORIGIN      
        1 cggagtcata ggcacggcag tcgccgcagg cgtcatgctt acgctgctga gctgaaaagc
       61 tacaggaatc tttatccgtt acctgaaggg agactcccgc aatccaggag tctccctttt
      121 ttcgaattcg tctgtgtttg tatatgcctt ttttccgaca aatgatatta tataaggatt
      181 gaagaggtca ccgggggctc gacgtttctt tctatctcct ggaaattata cgaggagggt
      241 ctgtgcatga agattgccgt gggttctgat catgctgcgt ttgctttgaa aaactatttg
      301 cttgagcatc ttcggcaaaa aagtggcatc gaaagcgtgg atatgggtgt ggatacggat
      361 gcggtttctt gtgattatcc ggatgtggcg ttccgtgttg gagaggccgt tgtttcccgc
      421 caggcggaca gaggccttct tttgtgcggt accggtgtcg gaatgagtat tgcagcgaac
      481 aagatccctg gagtgtatgc ggcccattgt catgacgtgg aaactgcccg attcagccgc
      541 cggcataata atgcgaatgt cctcaccatg ggggcgcgga taatatctca ggaacttgcc
      601 cgcgaaataa tcgatgtctg gctcgaaaca gattttgaag gagaccggca tataagaaga
      661 ttgagcaaga tccttgatta tgaacacaaa atctgtccaa gctgtacctt ttccgaaaaa
      721 ggcaagacgg tcgtctttga acacccgctt gtgcagcaca aggtaagcat catccgcgac
      781 aaaaatacct cggtgaaaga attccgtgag cttgtgcagg agattgcagg gctgatggtc
      841 tatgagatta cccgaaatct gccgctcgta aaaattgagg tagaaacccc tctcgcgaag
      901 acggaagcct acgcccttga ggggaaaaaa ctcgcgatag tacccgtcct cagagcgggc
      961 ctcggcatgg tcgacggcat tctccaactc gttccgaacg caaaagtcgg gcatatcggg
     1021 ctctatcgtg atcccgagac gcttctccct gtggagtact actgcaaact gcccggcgat
     1081 atcagcgaga gagatattct cgttctcgat ccgatgctcg ccacgggagg ttcggctgcc
     1141 gcagccatca gtctcgtcaa agcacggggc ggcaaaaagg tttcactcgt ttgcatcata
     1201 gcggctcctg aaggcgtctc caaggtgcac gccgagcatc cagacgttga tatctacgcg
     1261 gcggctcttg acagtcatct caatgaccac ggatatatcg ttcccgggct tggcgacgcc
     1321 ggagatcgtc tcttcgggac gaagtaaggg actttcgtgg attctctcgt tgcgcctctg
     1381 tcggtttcga taccgcttct tttcctctgg ggagtgtgcg ttacacctct ttcgatcctc
     1441 ctctccaatc ggtaccgtat agtcgaccag cctggggagc gtaaaattca tacttcagtc
     1501 actccccgag gagccggaat cgttctctgg ctaggatttc tcctatggag cctctatgca
     1561 gtggaggagg ctcctttgct tcgtttcatg gcggtaggtt ctaccctcgt gtttttaagc
     1621 ggttactggg acgatatgaa aactttgaac ccggccatac gactcacgtt tcatattgtt
     1681 gccgcaggtc ttttcctgct ttttctccct gtttctctgg ttcatatacc tgtgtgcctg
     1741 ctctggatta caggaatgac gaacgctttc aaccttatcg acggagcgaa cggcctgtgc
     1801 ctcatgatgt ttatcgtggc atcctcggtt tttgcagcgt gcgggttccc cttttttttc
     1861 cttccagtcg cgagcttgg
//
LOCUS       DAOTIL010000573         1877 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminobacteriaceae bacterium isolate AMR_MDS_3026
            NODE_73145_length_1877_cov_2.988474, whole genome shotgun sequence.
ACCESSION   DAOTIL010000573 DAOTIL010000000
VERSION     DAOTIL010000573.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868050
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminobacteriaceae bacterium (wastewater metagenome)
  ORGANISM  Aminobacteriaceae bacterium
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae.
REFERENCE   1  (bases 1 to 1877)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1877)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:19:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,224
            CDSs (total)                      :: 3,198
            Genes (coding)                    :: 3,184
            CDSs (with protein)               :: 3,184
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1877
                     /organism="Aminobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_73145_length_1877_cov_2.988474"
                     /isolate="AMR_MDS_3026"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:3034609"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1103)
                     /locus_tag="P5201_10920"
     CDS             complement(<1..1103)
                     /locus_tag="P5201_10920"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HRV99097.1"
                     /translation="MSLINVAHLTFAYEGGLENIFEDVCFRIDTDWKLGFTGRNGRGK
                     TTFLNLLLGRYEYEGTISSSVDFEYFPYDVPDEEMETLELIRSVAPGAEEWEIAREVS
                     LLDVADDALRRPFRTLSNGERTKVLLAALFLKENSFLLIDEPTNHLDMEARAAVTAYL
                     NRKKGFILVSHDRVFLDACIDHILSINRTNIEVQKGNFSSWWKNMQMREHYERSENER
                     LKKEIGRMQEAARRTSDWSDKVEKTKRGVRNSGLRPDTGYIGHKAAKMAQRSKSIDTR
                     RQSAIEEKSALLRNVEKSDELKLIPLVYHSPLLAELENVSIRYGERVACEGANFSIGR
                     GERIALQGKNGSGKSSILKLLHGESLEHCGRLF"
     gene            1232..>1877
                     /locus_tag="P5201_10925"
     CDS             1232..>1877
                     /locus_tag="P5201_10925"
                     /inference="COORDINATES: protein motif:HMM:NF015448.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004826 - phenylalanine-tRNA ligase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase beta subunit-related
                     protein"
                     /protein_id="HRV99098.1"
                     /translation="MFAASEEWKARYPDACFGVLIMEGVDNTGKCPALEERKDALEQS
                     LRTLYAGKSRKEIGAEHPFASYGRYYKTFGKNYHVLFQVESVALKEKPIFSPSPLVAV
                     MFMAELKNGLLTAGHDLDRTEPPFLLDVAKGGETFTSLGGTQRTLPPEDMFFSDRNGI
                     LSSIIYGPDDSSAITAATSRALFTVYGTPSISAGEVRAHLEEIEELVHILNPEAT"
BASE COUNT          372 a          578 c          536 g          391 t
ORIGIN      
        1 atgaacagcc ttccacagtg ctccagagac tctccgtgaa ggagcttgag aatgctcgac
       61 ttgccgctgc cgttcttccc ctgaagcgcg atgcgttccc cgcgcccgat ggagaaattc
      121 gccccttcgc acgcgacccg ctctccgtag cgtatggaga cgttctccag ctccgcaaga
      181 agcggggagt ggtagacgag ggggatgagc ttcagctcgt cgctcttctc cacgttccgc
      241 agcagcgccg acttctcttc gatggccgac tgccgccttg tgtcgatcga cttcgaccgc
      301 tgcgccatct tcgccgcctt gtgaccgata tacccggtgt ccgggcgcag gccggagttc
      361 cgcacgcccc gcttcgtctt ctcgaccttg tccgaccagt ccgacgtccg gcgggcggcc
      421 tcctgcatcc ggccgatctc cttcttcagg cgctcgttct ccgaccgctc gtagtgctct
      481 cgcatctgca tgtttttcca ccaggatgag aagttcccct tctgtacttc gatgttcgtc
      541 ctgttgatcg aaaggatgtg atcgatgcac gcgtcgagaa agaccctgtc gtgcgagacg
      601 aggatgaagc ctttcttccg gttcaggtag gcggtcacgg cggcgcgcgc ctccatgtcc
      661 aggtggttgg tgggctcgtc gatcagcagg aagctgttct ccttcagaaa gagggcagcg
      721 agcaggactt tggtcctctc tccgttggag agcgtgcgga acgggcggcg gagcgcgtcg
      781 tccgccacgt cgaggagcga gacttcgcgg gcgatctccc actcctccgc gccgggcgcg
      841 acgctccgga tcagctccag cgtctccatc tcctcgtccg gaacatcgta ggggaagtac
      901 tcgaagtcca cgctcgacga gatggtcccc tcgtactcgt accgtccgag cagcaggttc
      961 aggaacgtgg tcttccctct gccgttcctg ccggtgaagc cgagcttcca gtcggtatcg
     1021 atccgaaagc agacgtcctc gaaaatattc tccaatccgc cctcgtaggc gaaggtaagg
     1081 tgcgcgacgt tgatcaaaga catgagcata tcctcctgtg aagaaaaaaa ccgcaagaag
     1141 gcctttctga ttttccgaat cccttcagga catccgcccg gatttttagt atcataaacc
     1201 gaaacgcttc gaaagaggag acgattgcac aatgttcgca gcatcagaag aatggaaggc
     1261 gcgctatccg gacgcctgct tcggcgtcct cattatggag ggcgttgaca atacggggaa
     1321 gtgccccgcg ctggaggagc gaaaggacgc tctggagcaa agtctccgca ctctctacgc
     1381 ggggaagagc agaaaggaaa ttggagctga acaccccttc gcatcctacg gacgatacta
     1441 caagaccttc gggaagaact atcacgtgct gttccaagtc gagtcggtgg cgttgaagga
     1501 aaagccgatt ttttcgccgt cgccgcttgt ggcggtcatg ttcatggccg aactgaaaaa
     1561 cgggctgctc accgccgggc acgacctcga ccggacagag ccgccctttc tcctggacgt
     1621 tgcgaaaggg ggagaaacct ttacttcact ggggggaacg caacgtactc ttcctccgga
     1681 ggacatgttc ttctccgacc ggaacggcat tctctcaagc atcatctacg gccccgacga
     1741 tagcagcgcc attactgcgg cgacctcccg cgcgctcttc acagtgtacg gaacgccgtc
     1801 tatttccgcc ggagaggtgc gcgcgcacct ggaggagata gaagaacttg tgcatatcct
     1861 gaacccggaa gccacac
//
LOCUS       DAOTIL010000574         1877 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminobacteriaceae bacterium isolate AMR_MDS_3026
            NODE_73159_length_1877_cov_2.357849, whole genome shotgun sequence.
ACCESSION   DAOTIL010000574 DAOTIL010000000
VERSION     DAOTIL010000574.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868050
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminobacteriaceae bacterium (wastewater metagenome)
  ORGANISM  Aminobacteriaceae bacterium
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae.
REFERENCE   1  (bases 1 to 1877)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1877)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:19:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,224
            CDSs (total)                      :: 3,198
            Genes (coding)                    :: 3,184
            CDSs (with protein)               :: 3,184
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1877
                     /organism="Aminobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_73159_length_1877_cov_2.357849"
                     /isolate="AMR_MDS_3026"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:3034609"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(183..893)
                     /locus_tag="P5201_10930"
     CDS             complement(183..893)
                     /locus_tag="P5201_10930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005661045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HRV99099.1"
                     /translation="MLLEISDLSVFYGDIRAVDGLSLSLKEGELVSVIGANGAGKSSL
                     LNCIMGIVPHKNGSISFSGTDISSLPVHKRAAKGIRFVPERARVFPRLSVYENILTGA
                     YGIRSKIDFPGKLQWLYSLFPVLEERRNQLASTLSGGEQQMLAISRALIAEPKLLLVD
                     EVSMGLMPVLVDTVFDVLKMLNREHGLSILLVEQNALASLGISHRSYVLETGRIVLEG
                     ASEELLKDPKVQEAYLGL"
     gene            complement(883..1650)
                     /locus_tag="P5201_10935"
     CDS             complement(883..1650)
                     /locus_tag="P5201_10935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006301063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HRV99100.1"
                     /translation="MDDMLLRLDNVSKHFGGLKAVDEVSFSLGRGEILGLIGPNGAGK
                     TTCFNLVSGVYAPTSGKIFFEGASTGGFPPHRMAALGVGRTFQVVKPFSTLSVLRNVM
                     VALGLRRYGGVLSSFARWETPAVRKEATALLEEVGIADLAEAPSGKLPLGNQRRLELA
                     RALALKPKLLLLDETFSGLRHEEVALLSRLVLDVRSKGIAVLLIEHNMKVAMGLSDRL
                     VVLDHGRLLAEGSAEDVRSNPRVVEAYLGKGASSHAS"
     gene            complement(1643..>1877)
                     /locus_tag="P5201_10940"
     CDS             complement(1643..>1877)
                     /locus_tag="P5201_10940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014163173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HRV99101.1"
                     /translation="MMVSMVVLGGIGTLWGPVLGALILGVLPEVFRPLVDYRMLLNAA
                     ILLLMIRFQPDGLLGEGSLLRRIFPKRGETSHG"
BASE COUNT          362 a          623 c          602 g          290 t
ORIGIN      
        1 gtctccgcgc cgaaggccag ggacgaaacc aatagtaccg cacacacgcc gagaagcacc
       61 gctttcataa tccgcattct ctttccacct ccataagaaa tttgcactgc ctttgaaaaa
      121 ccgttccgcc gcgttctatg gcgccgcctc cgggaagttc ccggaggata catccgaatc
      181 ggttacagcc ccaggtacgc ctcctgcacc ttcggatcct tcagcagctc ttccgaggcc
      241 ccttcgagga cgatgcgccc cgtctccagc acataggagc gatgggagat gccgagcgag
      301 gccagcgcgt tctgctccac caggaggatg gagaggccgt gttcgcggtt cagcatcttc
      361 agcacgtcga agaccgtgtc gacgagcacc ggcatcaacc ccatggagac ctcgtccaca
      421 agcagcagct tcggctccgc gatcagcgcc ctcgaaatgg cgagcatctg ctgctccccg
      481 ccggagagcg tggaggcgag ctggttcctg cgctcctcaa gcaccggaaa gagcgagtag
      541 agccattgca gctttccggg gaagtcgatc ttcgaccgga tgccgtacgc tccggtgagg
      601 atgttctcgt agaccgagag gcgcggaaag acccgcgccc gctccggcac gaagcggatg
      661 cccttcgccg cgcgtttgtg cacgggcagg gaggagatgt ccgtacccga gaaggaaatc
      721 gagccgtttt tgtgaggcac gatccccatg atgcagttca gcagggagct cttccccgcg
      781 ccgttggccc cgatgaccga gaccagctcc ccctccttca gcgagagaga gaggccgtcg
      841 acggcgcgga tgtccccgta gaagacggag aggtccgaaa tctcaagaag catgggagga
      901 cgcccccttt ccgagatagg cctcgaccac gcgggggttc gaccggacgt cctccgcgct
      961 cccctcggcg agcagtctgc cgtggtcgag caccacgagg cggtcggaga gccccatggc
     1021 gaccttcatg ttgtgctcga tgaggagcac cgcgatcccc ttgctccgga cgtcgagcac
     1081 aagccgcgag aggagcgcga cctcttcgtg gcgcaggccg gagaaggtct cgtcgagcag
     1141 caggagcttc ggcttcagcg cgagcgcgcg ggcgagttcg aggcggcgct ggttccccag
     1201 cggcagcttc cccgagggcg cctccgccag gtcagcgatg ccgacctcct caagaagcgc
     1261 cgtcgcctcc ttccgcaccg ccggggtctc ccaccgggcg aatgacgaga ggacgccgcc
     1321 gtagcggcgc agccccaggg ccaccatgac gttccgcaat accgagaggg tggagaaggg
     1381 cttgaccact tgaaaggtgc gcccgacgcc gagcgcggcc atcctgtgcg gggggaatcc
     1441 cccggtggag gccccctcga agaagatctt tccggaagta ggggcgtaca cccccgagac
     1501 gaggttgaaa caggtggtct tccccgcgcc gttggggccg atgaggccga ggatctcccc
     1561 cctgccgagg gagaaggaga cctcgtcgac cgccttcagg ccgccgaagt gtttcgagac
     1621 gttgtcgagg cgaagcagca tatcatccat gagacgtttc ccccctcttc gggaagatgc
     1681 gccgcaggag gctcccctcg ccgagcagcc cgtcgggctg gaagcggatc atcagcagca
     1741 ggatcgccgc gttgagcagc atccggtagt cgacgagcgg acggaagacc tcggggagca
     1801 ccccgaggat gagcgcgccg agcaccggcc cccagagggt gccgatgccg ccgagcacca
     1861 ccatggacac catcatg
//
LOCUS       DAOTIL010000575         1876 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminobacteriaceae bacterium isolate AMR_MDS_3026
            NODE_73198_length_1876_cov_3.232839, whole genome shotgun sequence.
ACCESSION   DAOTIL010000575 DAOTIL010000000
VERSION     DAOTIL010000575.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868050
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminobacteriaceae bacterium (wastewater metagenome)
  ORGANISM  Aminobacteriaceae bacterium
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae.
REFERENCE   1  (bases 1 to 1876)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1876)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:19:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,224
            CDSs (total)                      :: 3,198
            Genes (coding)                    :: 3,184
            CDSs (with protein)               :: 3,184
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1876
                     /organism="Aminobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_73198_length_1876_cov_3.232839"
                     /isolate="AMR_MDS_3026"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:3034609"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..680
                     /locus_tag="P5201_10945"
     CDS             <1..680
                     /locus_tag="P5201_10945"
                     /inference="COORDINATES: protein motif:HMM:NF013705.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DnaJ C-terminal domain-containing protein"
                     /protein_id="HRV99102.1"
                     /translation="GWGGGVHMDFGGDGGGFSDFFRTIFGGFASGGRGVSMDDLFRSQ
                     GRGGDEEMSLELSLEDILRGGTKTISMEQVVHGADGRPSRSRRTLNVNLPQGVTEGSR
                     IRLKGQGSRGADLHIVIHAAPHPKFTVEGYDLVTSLKVSPWEAALGASIPVTTLDGVV
                     SMKLPAGVQAGKRLRLKEKGLPRRKGAQAGDLIVKVEVVVPEKLSDRERELFEALAKE
                     SSFNPRG"
     gene            complement(664..>1876)
                     /locus_tag="P5201_10950"
     CDS             complement(664..>1876)
                     /locus_tag="P5201_10950"
                     /inference="COORDINATES: protein motif:HMM:NF013100.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="penicillin-binding transpeptidase
                     domain-containing protein"
                     /protein_id="HRV99103.1"
                     /translation="FAYLAAFEKGILTPASLLADTPLAFSGQAPRNFDLTYRGPVSAR
                     IALADSLNVPAVRVLRAAGPELVLDLLRNAGFLSLTQSTAHYGDSLILGGCETSVLQM
                     AEGYVMLATLGIRRPPRFLPSEPPFHRRLVSEGAAFLVADILKDTGRLLPLHGKRIEY
                     GKEWFAFKTGTSYGYRDAWTAAYTPRNTVVVWMGNPTGKAHPELVGSQAAAPAIVEIL
                     RGLPPSGWYDPPQQVIMRTVCALSGRPIAPACPVGRLDYALEGISSSVPCPMHVIRDG
                     SARTVWPAELEEFALRRSLTLQAEQKATITSPLPGSRYILTPLGGDQRVALKAEGAKP
                     PVYWFVGGEYAGKQEGKEALFWTLKKGVHAVSLVDAAGRTAVSRFTVDAPGVKGPDES
                     GPPPLLLNPGD"
BASE COUNT          352 a          571 c          626 g          327 t
ORIGIN      
        1 ccggatgggg cgggggcgtg cacatggatt ttggcggcga cgggggcggg ttcagcgact
       61 tcttcagaac gatcttcggc ggcttcgcct cgggcggcag aggcgtttcg atggatgatc
      121 tcttccgttc gcaggggcgt ggcggcgacg aggagatgtc tctcgaactc tcgcttgagg
      181 atattctccg gggagggacg aagaccattt cgatggagca ggtggtgcac ggcgcggacg
      241 gacgtccgtc ccgaagccgg aggacgctca acgtgaacct tccccagggc gtgaccgagg
      301 gctcgcgcat caggctcaaa gggcagggaa gcagaggcgc cgatctgcac atcgtcatcc
      361 atgcggcgcc ccatcccaag ttcaccgtcg aggggtacga tcttgtcact tccctgaagg
      421 tctctccatg ggaggccgcc cttggagcat ccatccccgt gacgacgctc gacggcgtgg
      481 tctcgatgaa gcttccggca ggagttcagg cgggaaagcg gctccgtttg aaagaaaaag
      541 ggctccccag aagaaaggga gcccaagcgg gcgatcttat cgtgaaagtc gaggtcgtcg
      601 tgcccgagaa actgagcgac cgcgagcggg agttgttcga agcgctcgca aaagagtcgt
      661 cgttcaatcc ccggggttga gaagaagcgg cggagggccg gattcgtccg gtcccttcac
      721 gcccggggcg tcgacggtaa accgcgaaac ggcggttctt ccggcggcat ccaccagcga
      781 aacggcgtgc acccctttct tgagcgtcca gaagagcgct tcctttccct cctgttttcc
      841 ggcatattct cctcccacga accaatagac cgggggcttg gctccctcgg ccttcaacgc
      901 caccctctga tcccctccga gcggtgtgag aatataccgc gagccgggga gcggagaggt
      961 gatcgtcgct ttctgctccg cctggagcgt cagcgaacgg cgcagagcaa actcttccag
     1021 ttcggcgggc cagactgtcc gtgccgatcc gtctctgatg acgtgcatcg ggcacggcac
     1081 ggaggacgaa ataccctcaa gagcgtagtc caaccttcct acagggcagg cgggagcgat
     1141 cggccggccc gatagagcgc acaccgtgcg catgataacc tgctgcggag ggtcgtacca
     1201 ccccgaggga gggaggccgc ggaggatctc gacgatggcg ggagcggcgg cctgcgagcc
     1261 cacgagctcc ggatgcgcct tccccgttgg attgcccatc cagacgacga cggtgttgcg
     1321 cggcgtatag gcggcggtcc aggcgtcgcg gtagccgtac gaggtacctg ttttgaaggc
     1381 gaaccactct ttcccgtact ctatccgctt gccgtgcagg gggaggagtc gtccagtgtc
     1441 tttgaggata tcggcgacca gaaacgccgc tccttcggag accagcctcc tgtggaatgg
     1501 aggttcgctc gggaggaaac gcggcgggcg gcgtatcccc agcgtcgcca gcatgacgta
     1561 cccctccgcc atctgaagaa cgctcgtttc gcaccctcca agaatcagcg aatctccata
     1621 atgggccgtg ctctgcgtca ggctcaggaa acccgcgttg cgaaggagat cgagcaccag
     1681 ctccggcccc gccgcccgaa gtaccctgac tgccgggacg ttgagcgagt ccgcgagggc
     1741 tatcctggct gaaaccggtc ccctgtaggt caggtcgaag ttccgcggcg cctgtccgga
     1801 gaaggcgagc ggagtgtccg ccaggagcga cgcgggggtg agaatcccct tctcgaaggc
     1861 tgcaagatag gcgaac
//
LOCUS       DAOTIL010000576         1871 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminobacteriaceae bacterium isolate AMR_MDS_3026
            NODE_73485_length_1871_cov_3.634361, whole genome shotgun sequence.
ACCESSION   DAOTIL010000576 DAOTIL010000000
VERSION     DAOTIL010000576.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868050
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminobacteriaceae bacterium (wastewater metagenome)
  ORGANISM  Aminobacteriaceae bacterium
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae.
REFERENCE   1  (bases 1 to 1871)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1871)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:19:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,224
            CDSs (total)                      :: 3,198
            Genes (coding)                    :: 3,184
            CDSs (with protein)               :: 3,184
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1871
                     /organism="Aminobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_73485_length_1871_cov_3.634361"
                     /isolate="AMR_MDS_3026"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:3034609"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1545
                     /locus_tag="P5201_10955"
     CDS             <1..1545
                     /locus_tag="P5201_10955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009200535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent enzyme"
                     /protein_id="HRV99104.1"
                     /translation="AILGDDPGANSSQNEQDNRHIARLAYIPVLEPASPAEAYTFYLE
                     AMKLSRKRKMPVILRMTTHVCHGKEKVSFAGWTPRPIGSVPAFDSSAGPSIPLTTRAL
                     DMKRRAIARLAEVAQETDGSPLHELREGNDPSKGVITAGLPFRSLVDVLEDAAEKPDI
                     LKLGVVHPLPKGLVADFLRSHASVKVLEELDDVLEREIKALAFDLGIRTVIVGKTDPE
                     DWIGEYTPDKVAEVLAKTWPSLVPRKSAERAATLAPRPPQMCPGCGHRSAFHAIKQAL
                     AKEDITVADIGCHTLGFLPPYRMGEVLLSMGHSTGTAAGLSLFNTTRKVVAFLGDSTF
                     FHAGLPGIVNAVFNNHNLTLILMENGTTAMTGHQDHAGSGGNANGPADAIPVRAVLEG
                     LGVKSIREADTYSQAKLTAMVKEAVAEEGFSVVIARHPCMLKYTREQQRTPGYVRKSV
                     NIDQEKCDKLHVCVESFGCPSFQRDEEGRVVVSEELCIGDGSCIQTCPAKAIGVKKVQ
                     EGGGKA"
     gene            1542..>1871
                     /locus_tag="P5201_10960"
     CDS             1542..>1871
                     /locus_tag="P5201_10960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014807270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016903 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoacid:acceptor oxidoreductase family
                     protein"
                     /protein_id="HRV99105.1"
                     /translation="MSRFDIYMSGVGGQGIGLLSEAIIRAADYAGLAVCGVDTHGLAQ
                     RGGMVESFVRIGTLTTPLVRKGSAKLVLALERTEALRAVELFAAPGGTLLYYDTCWQP
                     LSVRLGTE"
BASE COUNT          335 a          650 c          600 g          286 t
ORIGIN      
        1 gccatcctcg gcgacgatcc cggagccaat tcctcgcaaa acgagcagga caaccgccac
       61 atcgcccgcc tcgcctacat ccccgtgctc gaacccgcct cgcctgcgga ggcctacacg
      121 ttctaccttg aggcgatgaa gctctctaga aagcggaaga tgcccgtcat cctccggatg
      181 accacgcacg tctgccacgg caaggagaag gtctccttcg cagggtggac gccccgtccg
      241 atcgggagcg ttcccgcgtt cgactcctcc gcagggccgt ccattccgct caccacgcgc
      301 gcactcgaca tgaaacggcg cgcgatcgcc cgtctggccg aagtggcgca ggagaccgac
      361 ggctcgccgc tccacgaact gcgcgagggg aacgatccgt cgaagggcgt catcacggcg
      421 gggcttccct tccgctcgct ggtcgacgtg ctggaggacg cggcggaaaa gccggatatc
      481 ctcaagctcg gcgtggtgca ccctctcccg aagggactcg tggccgactt cctgcgctcc
      541 cacgcctccg tgaaggtgct cgaagagctg gacgacgtgc tcgaacgcga gatcaaggcg
      601 ctggccttcg acctcggcat acgcaccgtg atcgtcggca agaccgaccc cgaggactgg
      661 ataggggagt acactcccga caaggtggcg gaggtgcttg cgaagacctg gccctccctc
      721 gtcccgcgca aaagcgcgga gcgcgcggct acccttgccc cccgtccgcc gcagatgtgc
      781 cccggatgcg gtcaccgctc ggccttccac gccatcaagc aggccctcgc gaaggaggac
      841 atcaccgtcg cggacatcgg ctgccacact ctcggtttcc ttcctcccta caggatgggg
      901 gaggtgctcc tctcgatggg gcactccacc ggcacggcgg cggggctctc gctcttcaac
      961 accacgcgca aggttgtcgc ctttctcggc gactccacct tcttccacgc cggacttccc
     1021 ggcatcgtca acgcggtctt caacaaccac aacctcacgc tcattctgat ggagaacggc
     1081 accaccgcca tgacggggca ccaggaccac gcgggctcgg gcggcaacgc caacggcccc
     1141 gccgacgcca tccccgtccg tgcagtgctc gaagggctgg gggtgaagag catccgcgag
     1201 gcggacacct acagccaggc gaagctgacg gccatggtga aggaggccgt cgcggaggag
     1261 gggttctctg tcgtcatcgc ccgccacccg tgcatgctga agtacacgcg cgagcagcag
     1321 cgaacaccgg gatacgtccg taaatcggtg aatatcgacc aggagaagtg cgacaaactg
     1381 cacgtctgcg tcgagtcctt cggctgcccc tcgttccagc gcgatgaaga gggacgcgtc
     1441 gtcgtgagcg aagagctgtg catcggcgac ggctcctgca tccagacctg tccggcgaag
     1501 gcgatcggcg tgaagaaagt tcaggaagga ggcgggaagg catgagccgc ttcgatatct
     1561 acatgtcggg cgtcggcggc caggggatcg gtctgctgag cgaggcgatc atcagggccg
     1621 cggactacgc ggggctcgcg gtctgcggcg tggacacgca cggcctcgcc cagcggggcg
     1681 gcatggtcga gtcgttcgtc cgcatcggga cgctgacgac gccgctggtc cgcaagggga
     1741 gcgccaagct cgtgctcgcc ctcgaacgca ccgaggcgct gcgggccgtg gagctcttcg
     1801 ccgcgcccgg aggaacgctg ctctactacg acacatgctg gcagccgctc tccgtccgcc
     1861 tcggcacgga g
//
LOCUS       DAOTIL010000577         1871 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminobacteriaceae bacterium isolate AMR_MDS_3026
            NODE_73508_length_1871_cov_2.780286, whole genome shotgun sequence.
ACCESSION   DAOTIL010000577 DAOTIL010000000
VERSION     DAOTIL010000577.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868050
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminobacteriaceae bacterium (wastewater metagenome)
  ORGANISM  Aminobacteriaceae bacterium
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae.
REFERENCE   1  (bases 1 to 1871)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1871)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:19:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,224
            CDSs (total)                      :: 3,198
            Genes (coding)                    :: 3,184
            CDSs (with protein)               :: 3,184
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1871
                     /organism="Aminobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_73508_length_1871_cov_2.780286"
                     /isolate="AMR_MDS_3026"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:3034609"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..451
                     /locus_tag="P5201_10965"
     CDS             <1..451
                     /locus_tag="P5201_10965"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="rRNA (cytidine-2'-O-)-methyltransferase"
                     /protein_id="HRV99106.1"
                     /translation="LFYGFLPDKQGERERAILSVASVPWTLLFYVSPHKAEKHLLHLC
                     ALLGDRRAALVREISKIYEEARRGVLSALAESVRGGVKGELVLVVEGSSGERGPSAEL
                     PEWRERAASLASEGVSSKEVVKIVSEEYSVPKNEVKSFLFQESGGNQ"
     gene            553..>1871
                     /gene="metG"
                     /locus_tag="P5201_10970"
     CDS             553..>1871
                     /gene="metG"
                     /locus_tag="P5201_10970"
                     /EC_number="6.1.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012869940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000049 - tRNA binding [Evidence IEA];
                     GO_function: GO:0004825 - methionine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006431 - methionyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine--tRNA ligase"
                     /protein_id="HRV99107.1"
                     /translation="MNEKKSFYLTTPIYYVNDIPHIGHAYTTIAADAMCRYKRMKGFD
                     VFFLTGTDEHGQKIQQSAAAKGLTPRELADRTVRNFQDLWKALNISNDDFIRTTEERH
                     YKTVQSIFKKLLDQGDIYKGKYEGWYCVPCETYVPESSMGEDKTCPDCKRPLQMMEEE
                     SYFFRASKYVDQLLAYYEKNLKGVMPRSRYNEIISFLKGGVRDQSISRTTLKWGIPVP
                     GDDAHVVYVWFDALINYVTACGYLDDRKKFDTFWPEVRHLVGKDIIRFHCVIWPIMLL
                     ALGVNPPVSVFAHGWWTVEGEKMSKSKGNVVDPFEMVERYGADPFRYFLLREVPFGLD
                     GDFSESAVVGRINSDLANDLGNLLNRTLQMIDNFFQGSLPASCRRGEREEEIVQRAAA
                     VLAEVDEKIEDFAFDDALKSIWSFISRGNKYIDETMPWKLAKEGKTE"
BASE COUNT          401 a          557 c          573 g          340 t
ORIGIN      
        1 tctcttctac ggttttcttc cggacaaaca gggggaacgc gagcgggcga tcctctccgt
       61 ggcctccgta ccgtggacgc tcctttttta cgtctcgccg cacaaggcgg agaaacacct
      121 tctgcacctc tgcgcacttc tgggagaccg cagggcggcg cttgtccgcg agatcagcaa
      181 gatctacgag gaggcgcgcc gcggcgttct ttcggcgctt gcggagtcgg tgcgcggcgg
      241 cgtgaaggga gagctcgtcc tcgttgtcga gggatcgtcc ggggagcgcg ggccttcagc
      301 ggaactgccg gagtggagag agcgcgccgc atcgcttgcg tcggagggag tctcctctaa
      361 agaggttgtc aaaattgtct cggaggagta ttctgtaccg aaaaacgaag tcaaatcgtt
      421 ccttttccag gagtccggcg gaaatcaata acgtaccgca tcgcgggcgg cgattcagcc
      481 ggacggcgtg aaccgttccg cgctcagtcc gttctcgtga aggagcagaa atttgcagga
      541 ggtatcgcaa acatgaacga aaagaagagc ttctacctca ccaccccgat ctactacgtc
      601 aacgacatcc cgcacatcgg gcatgcctac acgaccatcg ccgccgacgc gatgtgcagg
      661 tacaaaagga tgaaggggtt cgacgttttc ttcctcaccg ggaccgacga gcacggacag
      721 aagattcagc agagcgccgc agccaagggg ctcacgccgc gcgaacttgc cgacaggacg
      781 gtgcgcaact tccaggatct ctggaaggcg ctcaacatct ccaacgacga cttcatccgc
      841 accaccgagg agcgtcacta caagacggtg cagtccatct tcaagaagct cctcgatcag
      901 ggcgacatct ataaagggaa gtacgagggg tggtactgcg tcccctgcga gacgtacgtt
      961 cccgagagca gcatgggcga ggacaaaacc tgtcccgact gcaaacggcc tctccagatg
     1021 atggaggagg agagctactt cttccgcgcg tcgaagtacg tcgaccagct cctggcctat
     1081 tacgaaaaga atttgaaggg cgttatgccg cgcagccgct acaacgagat catcagcttc
     1141 ctcaaggggg gcgtgcgcga ccagtccatc tccagaacca ccctgaagtg ggggatcccg
     1201 gtgcccggcg acgacgcgca cgtggtgtac gtctggttcg acgcgctgat caactacgtg
     1261 acggcctgcg gctacctcga cgaccggaag aagttcgaca ccttctggcc ggaagtgcgc
     1321 catctcgtcg gcaaggacat catccgcttc cactgcgtga tctggcccat tatgctcctc
     1381 gcgctcggcg tcaaccctcc cgtttccgtg ttcgcccacg gctggtggac cgtcgagggg
     1441 gagaagatgt cgaaatcgaa ggggaacgtt gtggacccct tcgagatggt ggagcgctac
     1501 ggcgcggacc ccttccgcta cttcctgctc cgcgaggttc cgttcggcct cgacggcgac
     1561 ttctccgaga gcgcggtagt ggggcggatc aactccgatc ttgcgaacga cctggggaat
     1621 ctgctcaaca gaactctcca gatgatcgac aacttcttcc agggatcgct tcccgcatca
     1681 tgcaggaggg gggagcgcga ggaggagatc gtacagcggg ccgccgccgt cctcgccgag
     1741 gtcgacgaaa aaatcgagga cttcgccttc gacgacgcgc tgaagagcat ctggagcttc
     1801 atctcgcgcg ggaacaagta tatcgacgag accatgccgt ggaagctcgc caaggagggg
     1861 aagaccgaag a
//
LOCUS       DAOTIL010000578         1866 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminobacteriaceae bacterium isolate AMR_MDS_3026
            NODE_73752_length_1866_cov_5.077305, whole genome shotgun sequence.
ACCESSION   DAOTIL010000578 DAOTIL010000000
VERSION     DAOTIL010000578.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868050
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminobacteriaceae bacterium (wastewater metagenome)
  ORGANISM  Aminobacteriaceae bacterium
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae.
REFERENCE   1  (bases 1 to 1866)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1866)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:19:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,224
            CDSs (total)                      :: 3,198
            Genes (coding)                    :: 3,184
            CDSs (with protein)               :: 3,184
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1866
                     /organism="Aminobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_73752_length_1866_cov_5.077305"
                     /isolate="AMR_MDS_3026"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:3034609"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..418)
                     /locus_tag="P5201_10975"
     CDS             complement(<1..418)
                     /locus_tag="P5201_10975"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRV99108.1"
                     /translation="MEDFARDVRRLLGWFRDELVPHFRQLLRFLGLPYCYLFLVNWKH
                     CRRSRLQVASDLLHAFFVLRNFPDNYSRCHLDERPRNEWALFYGSSYNPYQRRQLQRG
                     AQPREYQVLFEDKEVCQQLCHGTGLPVAPDVGVLSPH"
     gene            complement(473..1237)
                     /locus_tag="P5201_10980"
     CDS             complement(473..1237)
                     /locus_tag="P5201_10980"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HRV99109.1"
                     /translation="MSLESEKAFFDAGYEDSARASIWKFYAITRTSESKYYESILDTC
                     QGFDVLEYGCGPGAAAFPLAERGARVTGIDISEVAIRQAGEEAADRGLDARFEVMDAE
                     NLEYPDQSFDLVCGRGIIHHLDVAKSLAEVSRVLRPEGRAVFAEPLGHNPALWIFRRL
                     TPSYRTADEHPLVRKDLVAMKRAFREARIHYFHLVSFFAIPFLWTPFFSPVYGFLERV
                     DQVLFKLLPPLGLLSWYCVIDVKKPRVHAATSGPSA"
     gene            complement(1331..>1866)
                     /locus_tag="P5201_10985"
     CDS             complement(1331..>1866)
                     /locus_tag="P5201_10985"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRV99110.1"
                     /translation="LGNASTWDACTHREIQIPSAWSQDTVTVTVNQGTFQPGETAWLY
                     VTDADGNVNATGLEVQIGESGADDLPPNVAITFPTDTGSYGTLNPFVSLEGTADDDIG
                     LSELQWSNAATGDSGAVDKVSGDWSIWSVGDIPLALGQNSITVRARDTGGLVAVDIIV
                     VTYDPGPPDSPGQPMIF"
BASE COUNT          337 a          555 c          648 g          326 t
ORIGIN      
        1 ggtgggggga gagtacgccc acgtccggcg cgacgggcag acccgtgccg tggcagagct
       61 gctggcagac ctccttgtcc tcgaacagca cctggtactc gcgcggctgg gcgcccctct
      121 ggagctggcg ccgctggtag gggttgtaac tcgagccgta gaagagggcc cattcattcc
      181 gcggccgctc gtcgaggtgg cagcgcgagt agttgtcggg gaagttgcgc agcacgaaga
      241 aggcatggag gagatcgctg gcgacttgga gccgggaacg ccggcagtgc ttccagttca
      301 ccagaaagag gtagcagtaa ggcagaccca ggaagcggag cagctgtcgg aaatggggga
      361 cgagctcgtc gcggaaccag cccaggaggc gcctcacgtc gcgggcgaaa tcttccatga
      421 tcgttccttc actccgggat cggcgttcac ttgtcgcagg acggcggccg cctcaggcgg
      481 acgggcccga cgtcgccgcg tgcacgcgtg gtttcttcac gtcgatgaca cagtaccatg
      541 acagcagccc gagcggcggg agcagcttga agaggacctg gtccacgcgt tcgaggaaac
      601 catagacggg agagaagaat ggcgtccata ggaaggggat ggcgaagaac gagacgaggt
      661 ggaagtaatg gatcctggct tcgcggaagg cgcgcttcat ggcgacgaga tccttgcgga
      721 ccagggggtg ctcgtccgcg gtgcggtagc tcggcgtcag gcgtcggaag atccataggg
      781 ccggattgtg tccgaggggc tcggcgaaga ctgcacgtcc ctcgggtctc aggacccgcg
      841 agacttcggc tagggacttc gcgacgtcca ggtgatggat gatcccgcgt ccgcacacca
      901 ggtcgaagct ctggtccgga tactccaggt tctccgcgtc catcacctcg aaacgggcgt
      961 ccaggccgcg gtccgccgcc tcctcgccgg cctggcggat ggcgacttcc gagatgtcga
     1021 ttcccgtgac cctggccccc cgctcggcca gcggaaaggc cgcggcgccg ggaccgcaac
     1081 cgtactccag gacgtcgaac ccctggcagg tatcgaggat cgactcgtag tacttggact
     1141 cgctcgtcct ggtgatcgcg tagaacttcc agatggaggc gcgggcacta tcctcgtagc
     1201 cggcatcgaa gaatgctttt tcgctttcga gactcatctg tcactccgtc caaggaagtc
     1261 cgtggaacga gaaaggatga atcccgactc tactataata aaaggggtaa agaggaccga
     1321 aatcccagga ttagaagatc atgggttgtc ccggggagtc gggcgggccc ggatcgtagg
     1381 tcacgacgat aatgtcgacg gcgacgagtc cgccggtgtc gcgggcgcgg acggtaatgc
     1441 tgttctgccc cagcgccagc ggaatgtccc cgacgctcca gatgctccag tcgccgctga
     1501 ccttgtcgac cgctccggag tccccggtcg ccgcgttgct ccactgcagc tcgctcaggc
     1561 cgatgtcgtc gtcggcggtc ccctcgagag agacgaatgg gttcagggtc ccgtagcttc
     1621 ccgtatcggt cgggaaggtg atcgcgacgt tggggggaag gtcgtccgcg ccggattccc
     1681 caatctgcac ctcgagtccg gtcgcattga cgttgccgtc ggcgtcggtg acgtagagcc
     1741 aagcggtctc gccgggctgg aaggtgccct ggtttacggt gacggtgacc gtgtcctggc
     1801 tccaggccga ggggatctga atctcgcggt gggtgcaggc gtcccaggtc gacgcgttcc
     1861 ccagct
//
LOCUS       DAOTIL010000579         1866 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminobacteriaceae bacterium isolate AMR_MDS_3026
            NODE_73764_length_1866_cov_3.222529, whole genome shotgun sequence.
ACCESSION   DAOTIL010000579 DAOTIL010000000
VERSION     DAOTIL010000579.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868050
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminobacteriaceae bacterium (wastewater metagenome)
  ORGANISM  Aminobacteriaceae bacterium
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae.
REFERENCE   1  (bases 1 to 1866)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1866)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:19:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,224
            CDSs (total)                      :: 3,198
            Genes (coding)                    :: 3,184
            CDSs (with protein)               :: 3,184
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1866
                     /organism="Aminobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_73764_length_1866_cov_3.222529"
                     /isolate="AMR_MDS_3026"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:3034609"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..756)
                     /locus_tag="P5201_10990"
     CDS             complement(<1..756)
                     /locus_tag="P5201_10990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009164558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HRV99111.1"
                     /translation="MSHDYDVLVVGGGVVGCAIARELSRFKVSAAVLEKEMDVGLGTS
                     CRNSGVLHAGFNYAPGSLRAVLDVKGNAMMDDLCRDLKVKIKRIGKLTVALDEEDEGT
                     LQRLLEQGRANGVPGLEIIGKERMQAIQPGVEGYMALYSPTSSIISPYALTIGLAENA
                     LANGVNFHLGQQVVSVTPLAGKGWEVGTKSGERFTCAVLVNASGLFAADVCRMAGITE
                     YSIYPCRGEYYVLDKRLDGTLRALIYPAPKKNAP"
     gene            944..1702
                     /locus_tag="P5201_10995"
     CDS             944..1702
                     /locus_tag="P5201_10995"
                     /inference="COORDINATES: protein motif:HMM:NF019349.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FCD domain-containing protein"
                     /protein_id="HRV99112.1"
                     /translation="MNKTRKELIHRLSDLITEGDIVSEGKLPPERELAAILGTTRPLL
                     REALIALEALGVLDIRDRQGIFLAEEKVGEIGRVLGQAQVWPLELLSQVMEIRQLLDP
                     GAAALAALRRKERDIAKMDECIFLLEKLHADRDPQEALLGAYWNTVLHATIFKATGNT
                     LLSRLYESLLEMSEKGISAMRMEVLDSAAPERTEQILEQHRLLVSAIKEQDVKTAREA
                     SKKHLKFTIDTLVELSRVSPVSNFFAERMDSALE"
BASE COUNT          395 a          576 c          520 g          375 t
ORIGIN      
        1 cggcgcgttc tttttcggcg ccgggtaaat gagcgcgcgg agcgtcccgt cgagccgctt
       61 gtcgaggacg tagtattctc cacgacaggg gtagatcgag tattcggtaa tcccggccat
      121 tctgcagaca tccgccgcga agagcccgga agcgttcacc aaaaccgcac atgtgaaacg
      181 ctcccccgac ttcgtcccga cctcccaccc ctttccggcg agcggcgtga ccgagacgac
      241 ctgctgcccc aggtgaaagt tcaccccgtt ggccagcgcg ttttccgcaa ggccgatcgt
      301 aagcgcgtag ggcgagatga tcgagctggt gggcgagtag agcgccatat acccctccac
      361 acccggctga atcgcctgca tccgctcctt gccgataatc tccagaccag gaacgccgtt
      421 tgcccgcccc tgttcgagaa gacgctgaag cgttccttcg tcctcttcgt cgagggcaac
      481 cgtcagcttg ccgatacgct tgatcttcac cttgagatcg cggcagagat catccatcat
      541 tgcgtttccc ttcacgtcga gaaccgcacg cagcgatccc ggcgcatagt tgaatcccgc
      601 gtggagcacg ccgctgttcc ggcagctcgt accgagtccg acgtccatct ccttctccag
      661 gactgcggcg ctcaccttga agcgcgacag ctcccgggcg atggcgcacc ctactactcc
      721 accgccgacg acaagtacat cgtagtcatg cgacaacctg caacacctgc tttcaaagaa
      781 aaagacgttt ttttcgcata ctggtcgacc aaggtattcc aattatccgg cgactatagt
      841 atcatagcct ccaaagcaag acaagataaa tgcactgaaa atttcaagtt ctttttttaa
      901 caaatcgata gtgtcatgtc gatatctgct tggggaggcc tccatgaaca agactcgaaa
      961 agaattgatc cacaggttat cagatttgat cacggagggg gatatcgttt cggagggcaa
     1021 gctccccccc gaaagggagc ttgccgccat tctgggaacg acacgaccgc ttctccggga
     1081 ggcgctcatc gctctcgaag cgctgggcgt tctggatatc cgcgaccggc agggtatttt
     1141 tctcgccgaa gagaaggtcg gcgagatcgg gcgcgtcctg gggcaggccc aggtgtggcc
     1201 cctcgagctg ctctcccagg tgatggagat ccgccagctc ctcgatccgg gggcggccgc
     1261 gctcgccgcg ctccggcgca aggagcgcga catcgccaag atggacgagt gcatctttct
     1321 tctcgaaaag ctccacgccg accgcgatcc gcaggaggct ttgcttggag cctactggaa
     1381 cacggtgctc cacgcgacca tcttcaaggc gacggggaac acccttctct cacggctcta
     1441 cgagagcctg ctcgaaatgt ccgaaaaggg gatctcggcg atgcggatgg aggtgctcga
     1501 ctcagccgcg cctgagcgga cggaacagat ccttgagcag caccgcctgc tcgtctccgc
     1561 cataaaggag caggatgtga aaacagccag agaggcctcg aagaagcatc tcaagtttac
     1621 tatcgacacg ctggtggaac tgagcagggt ctctcctgtt tcgaactttt ttgccgagcg
     1681 gatggattcc gccctggagt gacgacacgg aaacagtgac acaaacacaa agaaggagcc
     1741 cttcaccgag agggctcctt ctttgtatgc tctctttctg ttctagtcga tcggagcaag
     1801 cccctgctcg tcccgggaga gaatgaagcg tgcgaggagc tgctgcatcc tctcggcgcg
     1861 ctccgc
//
LOCUS       DAOTIL010000580         1866 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminobacteriaceae bacterium isolate AMR_MDS_3026
            NODE_73786_length_1866_cov_2.154611, whole genome shotgun sequence.
ACCESSION   DAOTIL010000580 DAOTIL010000000
VERSION     DAOTIL010000580.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868050
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminobacteriaceae bacterium (wastewater metagenome)
  ORGANISM  Aminobacteriaceae bacterium
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae.
REFERENCE   1  (bases 1 to 1866)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1866)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:19:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,224
            CDSs (total)                      :: 3,198
            Genes (coding)                    :: 3,184
            CDSs (with protein)               :: 3,184
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1866
                     /organism="Aminobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_73786_length_1866_cov_2.154611"
                     /isolate="AMR_MDS_3026"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:3034609"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..279
                     /locus_tag="P5201_11000"
     CDS             <1..279
                     /locus_tag="P5201_11000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013047562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YedE-related selenium metabolism membrane
                     protein"
                     /protein_id="HRV99113.1"
                     /translation="HTNQMWNFLGMVLSGLAFTLAGGCPGRQVIMSGEGDGDSAIFVL
                     GMLVGAGFAHNFSLASSPAGVTAYGMTATAIGMVFCLAVGFLFRIKMD"
     gene            297..515
                     /locus_tag="P5201_11005"
     CDS             297..515
                     /locus_tag="P5201_11005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013047563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase TusA family protein"
                     /protein_id="HRV99114.1"
                     /translation="MADTVIVDARGLSCPQPVLETKKALEKLSGGRVEVLVDTVTSRE
                     NVARFGGSRGWKVEKEEREDGFCVVLAK"
     gene            complement(596..1372)
                     /locus_tag="P5201_11010"
     CDS             complement(596..1372)
                     /locus_tag="P5201_11010"
                     /inference="COORDINATES: protein motif:HMM:NF013429.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIM barrel protein"
                     /protein_id="HRV99115.1"
                     /translation="MKFPPHHTFALSAKAADPESRYPWALKHGFALEYTPDPEQLELL
                     PAHTAEFRRRNVPVRYHTRYFGLEFGDARAEYAEAAFDAHRRTLDAMAQVGGEVLTVH
                     AGLSKGVTLNSDTVARNLRRLADHGRSLGVTVSLENLRSGPASDPENILRWASSADTA
                     ITMDLGHVVSSEQVKSGELKGEEVARAFLPRLLEVHFYGREEGRHFPLESMKSVEHIV
                     EVVLESSCRWWTVELDNTAEALATKSLLEEYLREKGLLPA"
     gene            complement(1369..>1866)
                     /locus_tag="P5201_11015"
     CDS             complement(1369..>1866)
                     /locus_tag="P5201_11015"
                     /inference="COORDINATES: protein motif:HMM:NF019998.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TOBE domain-containing protein"
                     /protein_id="HRV99116.1"
                     /translation="EEAMSVADIVAVVRDGRLEQAGAPRELYDFPATPFVADFLGRVN
                     LIPATLSVDAVSLLGRSFPRPKTTFSDGPVLCALRPEKIALAPPEEGNLLGVVRKSTF
                     IGPVIRFIIDVGAERPLVVEESAASARTASPGESVSIRFSPEDLRLFGNTSPETAPMK
                     KEATP"
BASE COUNT          305 a          576 c          654 g          331 t
ORIGIN      
        1 cacactaacc agatgtggaa cttcctcggc atggtcctct cgggactcgc cttcacgctc
       61 gcgggaggct gccccggacg tcaggtgatc atgtccggcg agggcgacgg cgactcggcg
      121 atctttgtgc tcgggatgct cgtcggcgcg gggttcgcgc ataacttctc gcttgcgagt
      181 tcgcccgcgg gcgtgacggc gtacggaatg acggcgacgg cgatcggcat ggtcttctgc
      241 ctggccgtcg gtttcctgtt ccgcatcaag atggattgac gaggaggaat gtgaaaatgg
      301 ccgatactgt gatcgtcgac gcccgcggac tgtcgtgccc gcagcccgtc ctcgagacga
      361 agaaagcgct ggagaagctc tccggcgggc gcgtggaggt gctcgtggac accgtgacgt
      421 cgcgtgagaa cgtcgcccgc ttcggcggaa gccgcggctg gaaggtggag aaagaggagc
      481 gcgaagacgg tttctgcgtc gtcctcgcga agtagttcag cgtgagaaaa acatgcggcg
      541 ctcttcggag aaaactccgg agagcgccgt tttgttgtcc gtcgaggcag ccgcgtcagg
      601 cgggcaggag acctttttcc cgcagatact cttccagcag cgattttgtc gccagcgctt
      661 cggcggtgtt gtccagctcg acggtccacc agcggcacga cgactccagc acgacctcca
      721 cgatgtgttc gacgctcttc atgctctcca acgggaagtg ccgcccctct tccctgccgt
      781 agaagtgcac ctccagcagg cgcggcagga aggcgcgggc gacctcctcc cccttcagct
      841 cgcccgactt cacctgttcg gaggagacga cgtgccccag gtccatggtg attgcggtat
      901 cggcggagga ggcccagcgg aggatgttct ccggatcgct cgcggggccc gagcgcagat
      961 tctccaggct gacggtaacg ccgagcgatc ttccgtggtc ggcgagacgc cgcaggttcc
     1021 gcgcaaccgt gtcggagttc agcgtcactc ccttggagag tccggcgtgg actgtgagca
     1081 cctcgccgcc gacttgggcc atcgcgtcga gcgtgcggcg gtgcgcgtcg aaggcggctt
     1141 ccgcgtactc ggcgcgcgcg tcgccgaatt cgaggccgaa gtagcgcgta tggtagcgca
     1201 ccgggacgtt gcggcggcgg aattcggccg tgtgcgccgg cagaagctcc agctgctccg
     1261 ggtcgggagt gtactccagc gcgaagccgt gcttcagcgc ccaggggtag cggctctcgg
     1321 ggtccgcggc ctttgcggag agggcgaacg tgtggtgcgg cggaaatttc atggcgtagc
     1381 ctcctttttc atcggggcgg tctccggaga tgtgtttccg aaaagccgga ggtcctccgg
     1441 ggaaaagcgt atggagacgc tctctccggg tgaagcggtc cgcgcggatg cggcgctctc
     1501 ttcgacgacg agcggccgct ccgcccccac gtcgattata aagcgaatca cggggccgat
     1561 aaaggtcgac ttccgcacga cgccgagaag attgccctcc tccgggggag cgagcgcgat
     1621 cttctcgggc cgcagggcgc agaggacggg gccatcggag aacgtcgtct tcggtcgggg
     1681 gaaggatcgt cccagcaggg agaccgcatc gacggagagc gtcgccggga tcaggttcac
     1741 cctcccgagg aagtccgcga caaacggggt ggcgggaaag tcgtagagtt cgcgcggcgc
     1801 gcccgcctgt tccaggcgtc cgtcgcggac gacggccacg atgtcggcca cgctcatcgc
     1861 ctcctc
//
LOCUS       DAOTIL010000581         1865 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminobacteriaceae bacterium isolate AMR_MDS_3026
            NODE_73824_length_1865_cov_3.084530, whole genome shotgun sequence.
ACCESSION   DAOTIL010000581 DAOTIL010000000
VERSION     DAOTIL010000581.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868050
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminobacteriaceae bacterium (wastewater metagenome)
  ORGANISM  Aminobacteriaceae bacterium
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae.
REFERENCE   1  (bases 1 to 1865)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1865)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:19:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,224
            CDSs (total)                      :: 3,198
            Genes (coding)                    :: 3,184
            CDSs (with protein)               :: 3,184
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1865
                     /organism="Aminobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_73824_length_1865_cov_3.084530"
                     /isolate="AMR_MDS_3026"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:3034609"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..271)
                     /locus_tag="P5201_11020"
     CDS             complement(<1..271)
                     /locus_tag="P5201_11020"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRV99117.1"
                     /translation="MPYPLITRSVSDKNLPAPDSAVLAVSSTIKEYSGRSLFDVVFKK
                     TRREVRMMMLNRYGFGGVIFGNLFRDLRGKILGVHIESDLLWIQLV"
     gene            377..934
                     /locus_tag="P5201_11025"
     CDS             377..934
                     /locus_tag="P5201_11025"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRV99118.1"
                     /translation="MGERLWSAHSQKVRLVLDILPSSPETTSCGRFFDAAAALLGCGE
                     VVTFDGQAAMSLEAMARGTLIAPFLVTRQDGRFIIDWRPVIRWILDSMETMPAPDIAG
                     GIHGGLARAVADICGTLRKERGIRYAALSGGVWQNRRLAAITSIFLRRRGVEPLLHKL
                     LPPNDECVSAGQTVVAAEFFNHEER"
     gene            complement(1006..>1865)
                     /gene="glpX"
                     /locus_tag="P5201_11030"
     CDS             complement(1006..>1865)
                     /gene="glpX"
                     /locus_tag="P5201_11030"
                     /EC_number="3.1.3.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014806530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042132 - fructose 1,6-bisphosphate
                     1-phosphatase activity [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="class II fructose-bisphosphatase"
                     /protein_id="HRV99119.1"
                     /translation="NTVPMSGVVVIGEGEKDEAPMLFNGEQLGASDEPQMDIAVDPVD
                     GTRLTALGLAGAISVVALSERGTMFNPKNIFYMNKLVTGPDAADVIDINLSPEENIVR
                     VAQAKKKAVDDVTVVVLDRPRNDEIKDHIRKAGARIKLIPDGDIAGALLTCKDQGGAD
                     LLMGIGGSPEAVITACAIKCLDGNMQCKLWARNEGDAEESRKRGLDLDQVLTIHDLVQ
                     GDNIFFAATGVTDGAFLQGVRYEGRQIRTSSMVMRSKSGTIRFVDSIHVPKKLSELSK
                     VSGIDYSTV"
BASE COUNT          374 a          490 c          530 g          471 t
ORIGIN      
        1 ctacgagctg aatccagaga aggtcgcttt cgatatgcac ccccagtatc tttccacgca
       61 gatcgcgaaa aaggttgccg aaaattactc cgccgaaacc gtaccggttc agcatcatca
      121 tgcgcacttc gcggcgtgtc tttttgaaaa caacgtcgaa gagacttctg ccggaatact
      181 ctttgatggt actggatacg gcgaggacgg cactatctgg ggcggggaga tttttatcgg
      241 aaacagatcg cgttatgagc ggatagggca tttttacccc tcgcggcttc ccggaggaga
      301 cgcggctgta atggaaccat ggcgctatgc tcttggactt ttgcacgacg tatacgggaa
      361 ggaggaggcc tctgtcatgg gagaaaggct gtggagcgct cattcacaga aagtacgact
      421 ggttctcgac attctcccct cctccccgga aacgacatcc tgcggacgct tctttgatgc
      481 tgccgcagct ttgctggggt gcggagaggt cgtcactttt gacggacagg cggcgatgag
      541 tctggaggca atggccagag gaacgctgat agctcctttt cttgttacgc gacaagacgg
      601 ccgtttcatt atcgattgga ggcctgtgat ccgttggatt ctcgattcga tggagactat
      661 gcctgctccg gatatcgcgg gaggcatcca tggcggtctt gcccgggcag tcgcggatat
      721 ttgcggtacg ctccgtaaag agcgagggat tcgttatgct gccctttcgg gcggagtatg
      781 gcagaatcgg cgactcgcgg cgataacatc cattttctta cggcggcgag gagtggagcc
      841 tcttctccac aaactcttgc ccccgaacga cgagtgcgtc tctgcggggc aaactgtggt
      901 tgcggcggaa tttttcaatc atgaggagcg ataaaaaacg cctgcgggat atgcagagtg
      961 tcccgcaggc aaaagagggt ttccgcttct cttaagggct gtgtgtcaaa ccgtactgta
     1021 gtctattccg cttaccttgg agagctccga gagttttttc gggacatgga tgctgtccac
     1081 aaaacggata gtgccgcttt ttgagcgcat gaccattgag ctggtgcgaa tttggcgtcc
     1141 ctcataacgg acgccctgaa ggaacgctcc gtcggtgacc ccggttgccg caaagaatat
     1201 gttgtcgccc tgcacaaggt catgtattgt cagcacctga tcgaggtcga gtcctcgttt
     1261 tctgctttcc tcggcgtcgc cttcattgcg ggcccacagt ttacactgca tgtttccgtc
     1321 caggcacttg attgcacatg cggtgatgac tgcttcgggc gagccgccta ttcccataag
     1381 gagatcggcg ccgccctggt ctttacatgt aagaagggcg cctgcgatat cgccgtcggg
     1441 gatgagtttg atccttgcgc ctgcttttcg gatgtggtcc ttgatctcat cattgcgtgg
     1501 cctgtcgaga acgacaactg tgacgtcgtc cacggctttt tttttcgctt gcgccactcg
     1561 gacgatattc tcctccggtg aaagattgat atcgatgacg tctgctgcgt ccgggcctgt
     1621 aaccagtttg ttcatataaa aaatattttt cgggttgaac atcgttcctc tttccgagag
     1681 cgcgaccacg ctgatcgctc cggcaagtcc gagagccgtc aagcgagttc cgtcgaccgg
     1741 atccacagcg atgtccattt gcggttcgtc ggaagcgcca agctgttctc cgttgaagag
     1801 catgggggct tcgtcttttt cgccttctcc aatgacgaca accccggaca tcggaacagt
     1861 gttca
//
LOCUS       DAOTIL010000582         1862 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminobacteriaceae bacterium isolate AMR_MDS_3026
            NODE_73964_length_1862_cov_3.853348, whole genome shotgun sequence.
ACCESSION   DAOTIL010000582 DAOTIL010000000
VERSION     DAOTIL010000582.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868050
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminobacteriaceae bacterium (wastewater metagenome)
  ORGANISM  Aminobacteriaceae bacterium
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae.
REFERENCE   1  (bases 1 to 1862)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1862)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:19:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,224
            CDSs (total)                      :: 3,198
            Genes (coding)                    :: 3,184
            CDSs (with protein)               :: 3,184
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1862
                     /organism="Aminobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_73964_length_1862_cov_3.853348"
                     /isolate="AMR_MDS_3026"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:3034609"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..398
                     /locus_tag="P5201_11035"
     CDS             <1..398
                     /locus_tag="P5201_11035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013047773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HRV99120.1"
                     /translation="SSPITQALVAEYCAKGYLDSHLPVIIDNYRVKRDAMEKSFEKHL
                     APLGVTWVKPEGGFFYWLNVPSVNTNDLFKKAIEKKVAFVPGAPFCPVEGSGLHNARL
                     CYTFPSPEIIEDGVARLAEAIGELAIPAL"
     gene            586..>1862
                     /gene="mnmE"
                     /locus_tag="P5201_11040"
     CDS             586..>1862
                     /gene="mnmE"
                     /locus_tag="P5201_11040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013047772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis GTPase MnmE"
                     /protein_id="HRV99121.1"
                     /translation="MRPVNPSFQEETIAAIATARGEAGIAVVRLSGARSKEIADRTLS
                     LTLPLAETPARRMRNGYLLDEKGEALDQVLAVWFAAPKSYTGEDVVEIHTHGGTLVAS
                     KCLELLVCRGARLAEPGEFTKRAFLSGRIDLTQAEAVLGIIRSKSEEALRAATRTLRG
                     ELASFAMDIHQELLALSARMEVALDFPEEDIPLHDDDEILQSIYALCQSLEDLLDRCN
                     TGFLLREGIRVAIVGRPNVGKSSLLNALLKESRAIVTSIPGTTRDLIEEVLTYRGIPL
                     RLVDTAGMGGTPSDEVEALGIARAEATFEKADVRVWVVDGSSLPDEFDQSMSERIAGL
                     EHVVAVNKADLPRAKNAEEHTSEELLAEMLPGSTLLSISAKHGTGIDSLKDAIVSSIA
                     GAGTLDAGLNVSARQVEEIRAAIGSLKDASNALS"
BASE COUNT          441 a          527 c          496 g          398 t
ORIGIN      
        1 gctccagccc cattacacag gcgctcgtcg ccgaatactg cgccaaaggc tatcttgaca
       61 gccatctgcc cgttatcatt gacaattacc gggtcaagcg cgacgctatg gagaaaagct
      121 ttgaaaaaca cctcgctcct ttgggcgtta cctgggtcaa acccgaggga ggctttttct
      181 attggctgaa cgtgccttca gtaaatacga atgacctttt caaaaaagcc attgagaaaa
      241 aagtcgcctt cgttcccggc gctcctttct gtcctgtaga ggggtccgga ctgcacaatg
      301 cacgcctttg ctacaccttc ccgagtcctg agatcattga ggacggcgtg gcacgtttgg
      361 cggaggccat tggagaactc gccattcctg cgctgtaaag cagaaagaaa caaaagccgc
      421 acgaaagact gctttactct ctcttcgcta tactctctct atcttgaaca gaaaaacctc
      481 tcccgaaatg aagatttagg gagaggtttt cgtttttagt acagcgcttt tgcggtacaa
      541 tacgggacac agacagcaaa aatccacttt gagaggagga agaacatgcg cccggtcaat
      601 ccgtcgtttc aagaggaaac catcgccgca atcgcaacgg cccgggggga agcgggaata
      661 gccgtcgtac ggctttccgg ggcgcggtcc aaagagatcg ccgacagaac gctctcgctt
      721 accctccctt tagctgaaac acctgctcgc cggatgcgca acgggtacct tctcgacgag
      781 aagggagaag cccttgacca ggtgcttgcc gtctggttcg ccgctcccaa gagttacacc
      841 ggagaggacg tcgtggaaat acatacgcac gggggaacgc tcgtcgcctc gaagtgtctt
      901 gaactcctcg tttgcagagg cgcacggctt gctgaacccg gagaattcac caaacgcgcg
      961 tttctctccg gacggatcga cttgactcag gctgaagcgg tactcggcat catccgctcg
     1021 aagagtgagg aagcgcttcg agccgccacc agaacgctcc gcggtgaact cgcctcattt
     1081 gctatggata tccatcaaga actcctcgct ctctccgccc gaatggaggt agcgctcgat
     1141 tttcccgagg aggacattcc tctccatgat gacgatgaga ttctccaatc catttatgct
     1201 ctttgtcaaa gtcttgaaga ccttcttgac cggtgcaata ccggatttct tctccgggag
     1261 ggtatcaggg tggctattgt cgggcggccg aacgtaggga aatcatccct gctcaacgcg
     1321 ctccttaaag aatcccgcgc aatcgtcaca tcaattccgg gcacaacacg cgatctgatt
     1381 gaagaagtac tcacctatcg cgggattccg ctccgactcg tcgatacggc gggtatgggc
     1441 gggacgccct cggacgaagt agaagccctg gggattgccc gcgctgaagc taccttcgaa
     1501 aaagccgacg tcagggtgtg ggttgtggac ggaagttccc tccccgatga attcgaccag
     1561 tccatgtcgg agcgtattgc aggacttgaa catgtcgttg cggtaaacaa agccgaccta
     1621 ccgcgggcga aaaatgccga agaacacaca tcggaggagc tgctcgctga aatgctgccc
     1681 ggaagtacgc tcctttcaat ctctgcaaaa catgggacgg gaatcgattc gctgaaggac
     1741 gctatcgtct cctccatcgc cggcgcgggt acgcttgacg ccggattgaa cgtctcagcc
     1801 aggcaggtag aggaaattcg cgcagcaata ggctctctaa aagatgcctc gaacgcgctc
     1861 tc
//
LOCUS       DAOTIL010000583         1861 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminobacteriaceae bacterium isolate AMR_MDS_3026
            NODE_74027_length_1861_cov_2.844408, whole genome shotgun sequence.
ACCESSION   DAOTIL010000583 DAOTIL010000000
VERSION     DAOTIL010000583.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868050
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminobacteriaceae bacterium (wastewater metagenome)
  ORGANISM  Aminobacteriaceae bacterium
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae.
REFERENCE   1  (bases 1 to 1861)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1861)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:19:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,224
            CDSs (total)                      :: 3,198
            Genes (coding)                    :: 3,184
            CDSs (with protein)               :: 3,184
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1861
                     /organism="Aminobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_74027_length_1861_cov_2.844408"
                     /isolate="AMR_MDS_3026"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:3034609"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..605)
                     /locus_tag="P5201_11045"
     CDS             complement(<1..605)
                     /locus_tag="P5201_11045"
                     /inference="COORDINATES: protein motif:HMM:NF013088.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HRV99122.1"
                     /translation="MSGIRKSMSPLEWLLLLFLSVLWGGSFFFVGIVVHALPRFTIVA
                     LRVGLAALALHILLLLRGERIPLDRKLWGAFFVIGLLNNVIPFSLIIQGQMHIASGLA
                     SILNATTPLFTVVVAHFLTDDEKMTAGRFFGLLVGFSGVVLMIGPEAVAGLGTHVVAQ
                     LCVLGATLCYAFAGVFGRRFRGMKLAPLVAAAAVVVALLAWS"
     gene            complement(848..1327)
                     /locus_tag="P5201_11050"
     CDS             complement(848..1327)
                     /locus_tag="P5201_11050"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRV99123.1"
                     /translation="MSGRDDDLLAKELEKLAAAGGKIAAKISGKSSPAVTAEDSEIMR
                     KAARRLDTDQYREERMIEGDARSVLQLAYDALKNTGRILEENERESSVNPRISGVVGA
                     GFFGKNPAVVHAEIVGADEGRCTLRLTGAAKEGLIKQGTARKAVEKVLREFDSLKSS"
     gene            complement(1366..>1861)
                     /locus_tag="P5201_11055"
     CDS             complement(1366..>1861)
                     /locus_tag="P5201_11055"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HRV99124.1"
                     /translation="RRSLDENLETEWRRGMREHNFLAVVMLDIDLFKSYNDQYGHPSG
                     DECIRNIASVIKGYARRAGDIAARYGGEEFVIVLPGVDLGDAASIAEKIRLEIFSANR
                     PHAGSPEGRVTVSAGVASAPAASLDSGEALLRSADLALYRAKAEGRNRVVAASESARV
                     VAEK"
BASE COUNT          390 a          628 c          506 g          337 t
ORIGIN      
        1 gaccaggcga gcagcgcgac caccacggca gcggcagcga ccagcggggc gagcttcatc
       61 ccccggaagc gcctgccgaa cacgcccgcg aaggcgtagc agagcgtcgc ccccaggacg
      121 cagagctgcg cgacgacgtg cgttcccagc ccggcgacgg cctccggccc gatcatcagt
      181 acgacgccgg agaaaccgac gagcaggccg aaaaagcgcc cggcggtcat cttctcgtcg
      241 tcggtcagaa agtgcgcgac cacgacggtg aagagcggcg tggtcgcgtt gaggatcgag
      301 gcaagaccgc tggcgatgtg catctgcccc tggatgatca ggctgaaggg gatcacgttg
      361 ttcagcagcc ctatcacgaa gaaggcgccc cagagctttc tgtccaaagg aatgcgctcc
      421 cccctcaaga gaagaaggat atggagcgca agcgccgcca ggccgacgcg gagcgcgacg
      481 atggtgaagc gcggcagggc gtgaaccacg atgccgacga agaagaacga accgccccaa
      541 agaaccgaaa gaaagagcag caacaaccat tcgagcggac tcatcgactt tctgatgccg
      601 ctcatgacgc cgctccttcc ccgagccacg gcctgctggc tgccgagcgc ccacaagaac
      661 aacaaaagag acacccggtc ggaagaagag acgtcaacag agaacggatg cggcgcgttc
      721 gcttcatgcc cgctctgtat cggacactgt ttcgtttcac tcgcatagga acctcccgaa
      781 atagagaatg atgcttgatt ataccctccg tgcacacgga acgggaggcg tcctccggga
      841 gattcctcta cgaagacttc agggagtcga actcccgcaa taccttctcc actgcttttc
      901 ttgcagtccc ttgtttgatc agtccttcct tggccgcccc ggtgaggcgc agagtacaac
      961 gcccctcatc agcaccgact atctccgcgt ggaccaccgc aggatttttc ccgaagaaac
     1021 ccgcgcccac cacaccggaa atccgtggat tcacggagct ttcacgctcg ttttcctcca
     1081 ggattcggcc ggtgtttttc aaggcgtcgt acgcgagttg cagcacgctc ctcgcatccc
     1141 cctcgatcat acgttcctct ctgtactgat cggtatccaa tcgccgagcc gctttgcgca
     1201 tgatctcgga atcttccgcg gttacggcag gacttgactt gcccgatatc ttcgccgcga
     1261 tcttcccgcc cgccgccgca agcttttcaa gctccttcgc cagaagatca tcatcacgtc
     1321 cagacatacg cgacacctcc atcagcgttg ttcacactcc atgcctcact tttccgcaac
     1381 aacacgcgca ctctcgcttg ccgcgaccac cctgttgcgc ccttccgcct tcgcacggta
     1441 caacgccagg tccgcggaac gaagaagcgc ttcgccggag tcgagagagg ccgccggagc
     1501 ggaggccacc cccgcgctga cggtcacccg gccttcaggc gacccggcat gcggacggtt
     1561 tgcggagaat atctccaggc gaatcttctc cgctatcgac gcggcgtccc cgagatcgac
     1621 gcccggcagc acgatcacga attcctcgcc gccgtagcgc gcggctatat cccccgctct
     1681 ccgcgcatac cccttgatga cggaggcgat gttcctgata cattcgtctc cggacggatg
     1741 tccgtactga tcgttgtagc tcttgaaaag atcgatgtcc agcatgacga ctgccaggaa
     1801 gttatgttct cgcatacctc gtctccattc ggtctccaga ttctcgtcga gactcctgcg
     1861 a
//
LOCUS       DAOTIL010000584         1860 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminobacteriaceae bacterium isolate AMR_MDS_3026
            NODE_74069_length_1860_cov_3.266482, whole genome shotgun sequence.
ACCESSION   DAOTIL010000584 DAOTIL010000000
VERSION     DAOTIL010000584.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868050
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminobacteriaceae bacterium (wastewater metagenome)
  ORGANISM  Aminobacteriaceae bacterium
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae.
REFERENCE   1  (bases 1 to 1860)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1860)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:19:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,224
            CDSs (total)                      :: 3,198
            Genes (coding)                    :: 3,184
            CDSs (with protein)               :: 3,184
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1860
                     /organism="Aminobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_74069_length_1860_cov_3.266482"
                     /isolate="AMR_MDS_3026"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:3034609"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            1454..>1860
                     /locus_tag="P5201_11060"
     CDS             1454..>1860
                     /locus_tag="P5201_11060"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRV99125.1"
                     /translation="MKLAGKLLTVAERKETWLKRVLQGGKSFRESEPGKRCERWIGGV
                     WAAVRGRDYESGKRWSYREVVSAIQDIRKRRLVFSLVYTSVLAGTLTFLKLQGVQSDE
                     SASKILRTIFSIAPVVFALYIKILVARSYHYLSG"
BASE COUNT          462 a          490 c          489 g          419 t
ORIGIN      
        1 aaagagcgcg tcttcgtctt ctttcgcgac attctcgttc gcgaccgcat tcagcaccgt
       61 cttcacccgc agcagttcgt ttatccggct ttcaggcgag aggagccccc tctcttcaaa
      121 aaacccctgt acgtttttca tagtcttcgc ctccttcaat gcaaatagcc tacaccctta
      181 aatttagtat gtcaatacta aaaatgtttt tcaagaaatg aattttacaa tgcatggaag
      241 cagcaccgtg gaattgagct tccagacttc cgctttcctc atgtggaatg ttttcgaggg
      301 ctctaaacag acgtcggcgc tgtacgggaa gttctcagat cgaacaagcg aaaaaagagt
      361 gagaggatcc tcctgataaa aaaaccccgg actcaatgtc cggggctcaa cgcctttcgg
      421 cggtcagagg atgagagtgt tcgggagcct gcatcgacat cgcacaagaa atagtgctca
      481 acgcctgacg gcaatcggag gaagaaaagc gccgagataa aagctggtac gggaaactcc
      541 acgcgtatgg tacagatgag attattgtac caggaggaag gcacgcatgt ttctccgaac
      601 ctgcgaatca ctgtacagtt gggaggcaaa ggcatggagg gaatccatac acattcgata
      661 gagccgctgc tccattggcg ctacacgagg ttcctggaga cggcgacgtg aaagatccac
      721 ttaatacatt cgctgaattg cggacgtcga agacgcatcc tatcaagttg cattgttccg
      781 tcgacggaga agctcacagt gtgcgtcgta catctgcggc cggaggtggc gtagatcggc
      841 cgttcaaaga tctctcttgc cgtatcgagg agaataagca gcgcggcggc tgttgaagcg
      901 gcaccctgca gtttcgtaaa tctaaactcg tatgattgga agtccaggcg cgtagcgact
      961 taacatccac ttctccccga cgacacgagg tctttttgac cattctcgaa gataatacca
     1021 tagcaacgct tccctcttcc gcacagcgtt ttcctcgaag aaagccaccc ggatgatgat
     1081 agaatgagcg acggagactt tcgcgccaag cgaccgcggc gcgaatgcgc aagctccagc
     1141 agttgcgcaa gcgaaacata cggttctcgc actgcttctt tcgcattcgc tccctctggt
     1201 cattcgcctg atgcgttcgg agcagagtag attcgaaaca ctgcgaacac cctgaatgtc
     1261 tactcgaaag tcggctcgca aagaatgagg tgatgagaat gacaaaaata ggatcggagc
     1321 acgttgctcg ttttcgctgg cttgggaaaa ttttacttgt agatcttgtg aaaaattcat
     1381 tccgccgttc tccgtccctc ggcggctcag gagtcggaaa aacgccggtc cgacccgcta
     1441 cattcagctt ccaatgaagc tcgccggcaa gctgctcact gttgctgagc gtaaggaaac
     1501 gtggttaaag cgcgttctac agggagggaa aagctttcgc gaaagcgaac ctgggaaacg
     1561 atgtgaacga tggatcggag gggtctgggc cgccgtcagg gggcgcgact acgagagcgg
     1621 caagcgctgg agttatcggg aggtcgtctc cgccatacag gacattcgga agcgcaggct
     1681 ggttttttcg ctggtctaca cctccgttct cgccggaaca ctcaccttct tgaagctcca
     1741 gggggtgcag agcgacgaga gcgcctccaa gatcctgaga acgatcttct ccatcgcgcc
     1801 cgtcgtcttc gccctctaca tcaaaattct agtcgcgaga agctatcact atctctcagg
//
LOCUS       DAOTIL010000585         1860 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminobacteriaceae bacterium isolate AMR_MDS_3026
            NODE_74070_length_1860_cov_3.246537, whole genome shotgun sequence.
ACCESSION   DAOTIL010000585 DAOTIL010000000
VERSION     DAOTIL010000585.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868050
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminobacteriaceae bacterium (wastewater metagenome)
  ORGANISM  Aminobacteriaceae bacterium
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae.
REFERENCE   1  (bases 1 to 1860)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1860)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:19:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,224
            CDSs (total)                      :: 3,198
            Genes (coding)                    :: 3,184
            CDSs (with protein)               :: 3,184
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1860
                     /organism="Aminobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_74070_length_1860_cov_3.246537"
                     /isolate="AMR_MDS_3026"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:3034609"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..392
                     /locus_tag="P5201_11065"
     CDS             <1..392
                     /locus_tag="P5201_11065"
                     /inference="COORDINATES: protein motif:HMM:NF024811.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="HAD-IA family hydrolase"
                     /protein_id="HRV99126.1"
                     /translation="FRKRKLPMAIATGSNSSIAGEILTSLGVRDFFTMIVSSSEVKKG
                     KPEPDIFLETARRMAIDPKEAIVFEDTRFGVEAALRAGMTCVALPAPEDDADHKLFQQ
                     ADCLIKGGPDELDPQSFFWWFDTLAKL"
     gene            610..>1860
                     /locus_tag="P5201_11070"
     CDS             610..>1860
                     /locus_tag="P5201_11070"
                     /inference="COORDINATES: protein motif:HMM:NF028509.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cache domain-containing protein"
                     /protein_id="HRV99127.1"
                     /translation="MSKTSLPSQEPYSAVSGVNGTERRAWGTFVSTFLQTMAPVFFLI
                     VFLLVLLLYFFLPLLEESYLHQKRLMCKYLVQVQITYLEALHTDVLKGTISMEEAQGR
                     ALRRLRMHRFGEAEKDYFWALGPERTLVMHPYRPDLEGINPDEAKGPDGELLRGLFDG
                     IEAAAARTPEGGHLDYLWHFKDDLGTLSAKTSHVARFVPWNWIVGTGVYLDDIHMEIA
                     SWRRRFTLLGLFAVLGSGGLSLFLSIRAVLLGRKAALFVVADRSLREKTRTLIQTEKQ
                     LAIITSVYDHASEGVVITESDGTILRVNRAFTEITGFSKDEAIGRNPRILKSNTHDGE
                     FFERMWSDLLEKKRWSGEIWNRRKNGEAFPAMLNIVACSDDSGETTHYIGVFQDLSEL
                     HRSRDSLRHETFHDSLTGLPNRFLF"
BASE COUNT          380 a          580 c          523 g          377 t
ORIGIN      
        1 agttccgcaa gcgcaagctc cccatggcga tagcgaccgg ttccaactcc tccatcgccg
       61 gggagattct gacgtcgctc ggcgtgcgcg actttttcac catgatcgtc tcctcctccg
      121 aagtgaagaa ggggaaaccc gaacccgaca ttttccttga aacggcgcgc cggatggcca
      181 ttgatcctaa ggaagccatc gtcttcgagg atacccggtt cggcgtcgaa gccgcccttc
      241 gcgcgggcat gacctgcgtg gccctccccg ccccggaaga cgacgccgac cacaagctct
      301 tccaacaggc agactgtctg atcaagggcg ggccggacga gctggatccg caatcgttct
      361 tctggtggtt cgacaccctt gcaaaactct gattcacgct ccgcaccccc atccgcagag
      421 gcgacgcgta ccgtccatcg gcagatcccc gcgtcgcccc gcagattttt tgcatgaact
      481 gcaagttgct cctctctccg gaacagatat gagcctcaag attctcacgc agccgaacgc
      541 atctgataca atctttgtga acctccatgc tcagggcggt atgtaacagg agaaaggaga
      601 ttcctggaga tgtccaagac gtcgcttcct tctcaagaac cgtactccgc tgtttcgggc
      661 gtcaacggga cagagcgccg cgcatgggga acattcgtct cgacgtttct tcagaccatg
      721 gctcccgtct ttttcctgat cgtcttcctt ctcgtcctgc tcctttattt tttccttcct
      781 ctgctcgaag aaagctacct tcaccagaag cgcctgatgt gcaagtacct cgtgcaagtc
      841 cagattacct acctggaagc gctccatacc gacgtcctca agggaacgat ctccatggag
      901 gaagcgcagg gacgggccct tcgccgcctt cggatgcatc gcttcggaga ggctgaaaag
      961 gactacttct gggcgctggg cccggaacgg acgctcgtca tgcatccgta tcgtcccgac
     1021 ctggaaggca taaaccccga cgaggcgaag gggcctgacg gggagctgct gcgcggtctc
     1081 ttcgacggca tcgaggcggc ggcggcgcga accccggagg gaggacacct cgactatctg
     1141 tggcacttca aggacgatct ggggacgctc tcggccaaga cctcgcacgt tgcccgcttc
     1201 gttccgtgga actggatcgt cggaacgggc gtctacctgg acgacataca catggagatc
     1261 gcctcgtggc ggcggcgctt cacgctgttg ggactcttcg cggtgctcgg ctcgggcggt
     1321 ctttcgctct ttttgagcat tcgcgcggtc ctcttgggga gaaaggcggc gctcttcgtc
     1381 gtcgcggacc gttcgctccg cgagaaaacc cgcacgctca tacagacgga aaaacagctt
     1441 gcgattatca ccagcgtcta cgatcatgcg tcggaagggg tcgtcatcac cgaatccgac
     1501 ggcacgattc ttcgcgtcaa ccgggcgttc accgagatta ccggtttttc gaaggatgaa
     1561 gccataggaa gaaaccccag gattctcaag tcgaacacgc acgacggaga gttcttcgag
     1621 cgtatgtgga gcgaccttct ggagaaaaaa cggtggtccg gggagatttg gaacagacga
     1681 aagaacggcg aggcgttccc ggcgatgctg aacatcgtcg cctgcagtga cgactccgga
     1741 gagacaacgc actacatcgg cgtcttccag gacctcagtg aactgcaccg cagcagggac
     1801 agtcttcgcc acgagacgtt ccacgacagc ctcaccggac tgccgaaccg tttcctcttc
//
LOCUS       DAOTIL010000586         1860 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminobacteriaceae bacterium isolate AMR_MDS_3026
            NODE_74078_length_1860_cov_2.939058, whole genome shotgun sequence.
ACCESSION   DAOTIL010000586 DAOTIL010000000
VERSION     DAOTIL010000586.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868050
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminobacteriaceae bacterium (wastewater metagenome)
  ORGANISM  Aminobacteriaceae bacterium
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae.
REFERENCE   1  (bases 1 to 1860)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1860)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:19:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,224
            CDSs (total)                      :: 3,198
            Genes (coding)                    :: 3,184
            CDSs (with protein)               :: 3,184
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1860
                     /organism="Aminobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_74078_length_1860_cov_2.939058"
                     /isolate="AMR_MDS_3026"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:3034609"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1161
                     /locus_tag="P5201_11075"
     CDS             <1..1161
                     /locus_tag="P5201_11075"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRV99128.1"
                     /translation="LLLLFLAVTFFLAVRDDVIRLYAVDMAEEYRSAYLPVEGWGAST
                     AMRGVVRSSKRISSVAAFIDAHTEGRLEVVEGKSWEEIFAGVLAPPETSPLPDRRASD
                     TWRASYYFLPKEDPFPDVFREMKSEDQAFRFLKLSAPSGPRFIGMTVQGPEDAMSAGA
                     PSWILYPFRRFAAVPLLLALFVYLIFPPRKKAPAGALVYPKWSSWILPDLMGLLFSCI
                     FFAFPFFLAPAIFGSGNVLNIADGGIILTLVFWLMAAIFASILYWSAKYASFSLELLP
                     GAMMLRTLGKEQRIPYIAIESAGIADYRPPKWLRTTLFIASLGSWRMLGHALLLSSRS
                     DWGIELRMKHGDPVKFLCSNLPGAERIVEALRREGVNISPELESVFSERDDR"
     gene            1203..1556
                     /locus_tag="P5201_11080"
     CDS             1203..1556
                     /locus_tag="P5201_11080"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRV99129.1"
                     /translation="MYSSFKVLDGGAIRRSLHPETDLPIALHHGSAAFGNVGSGERLD
                     FTVVGRDVNLTDRIGRLNKTLGIPLLMSGDFAGVLDEPARSLGHHAVKSFDQSIEVFE
                     PARPRPGHPGEGAAT"
     gene            1553..>1860
                     /locus_tag="P5201_11085"
     CDS             1553..>1860
                     /locus_tag="P5201_11085"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRV99130.1"
                     /translation="MTLRYEKLTGAALKAVIADLAKLRIAVFRDWPYLYEGSVDYEAT
                     YLARYSDTPGALVVGAYDRGALVGASTATPLACEVEAFRTPFAEHGYDARRVFYFGES
                     "
BASE COUNT          293 a          613 c          593 g          361 t
ORIGIN      
        1 cttctgctgc tttttttggc cgtgacgttc ttcctcgccg tccgcgacga cgtgatccgt
       61 ctctacgccg tcgacatggc cgaggagtac cgctccgcgt atcttcccgt ggaggggtgg
      121 ggggcgtcca ccgcgatgcg gggcgtcgtc cgttcgtcga agcggatttc ctccgtcgcc
      181 gcgttcatcg acgcgcatac tgaggggcgg cttgaggtcg tcgaaggaaa gagctgggag
      241 gagatcttcg cgggcgtcct cgcgcctccc gagacgtcgc ctctgccgga caggagggcg
      301 agcgacacct ggcgggcctc ctactacttc ctcccgaagg aggacccctt cccggacgtg
      361 ttccgcgaaa tgaagagcga ggatcaagcc ttccgtttcc tgaagctctc cgcaccgtcg
      421 ggtccgcgct tcatcggaat gaccgtccag gggcccgagg acgcgatgag cgcgggcgcg
      481 ccgtcgtgga ttctgtaccc tttccggcgc tttgccgccg tgcccctgct cctcgcgctc
      541 ttcgtctacc ttattttccc gccgcgaaag aaagcgcccg cgggcgcgct ggtgtacccg
      601 aagtggtcgt cctggattct ccccgacctg atgggattgc tcttctcctg catcttcttc
      661 gcgtttccgt tcttcctggc gccggcgatc ttcggcagcg gcaacgtgct gaacatcgcc
      721 gacggcggga tcatcctcac gctcgtcttc tggctgatgg cggcgatttt cgcctccata
      781 ctttactggt cggcgaagta cgcctcgttc tcgctcgaac tgcttcccgg cgccatgatg
      841 ctccggacgc tcggcaagga acagcggatt ccgtacatcg ccatcgaatc cgccggtatc
      901 gccgactacc gccctcccaa gtggctgcgg acgacgctct tcatcgcatc cctgggaagc
      961 tggcgcatgc tggggcacgc gctgctcctc tccagccgga gcgactgggg gatcgagctg
     1021 cgcatgaagc acggcgatcc ggtcaagttc ctctgctcga acctgccggg cgcggagcgg
     1081 atcgtcgagg ccctccggcg cgagggcgta aacatctcgc cggaactgga gagcgtgttc
     1141 tccgaacgtg atgatcgata gaatgcaaaa aacgggagag gcgcaacgcc ctcccgtttt
     1201 tcatgtactc gtcttttaag gttctcgatg ggggcgcaat ccggcgcagc cttcaccccg
     1261 aaacggatct gcccatcgcc ctgcaccatg gcagcgcggc attcggcaac gtgggttcgg
     1321 gcgagcggct cgacttcaca gtggtggggc gtgacgtcaa cctgaccgac cggatcggcc
     1381 gtctgaacaa gaccctgggc atcccgctgc tgatgagcgg cgacttcgcg ggcgtgctcg
     1441 acgagccggc gcgctcgctg gggcaccatg cagtcaagag cttcgaccaa agcatcgagg
     1501 ttttcgaacc ggcccgacca cggccgggcc atccgggcga aggagccgcg acatgacgct
     1561 gcgctacgag aaactcaccg gagcggcact gaaggccgtg atcgcggacc ttgcgaagct
     1621 gcgcatcgcc gtgttccgcg actggccgta tctctacgaa ggcagcgtgg actacgaggc
     1681 gacgtacctg gcgcgctaca gcgacacgcc cggcgctctg gtggtggggg cctacgatcg
     1741 cggcgcgctg gtcggggctt cgacagcaac gccacttgca tgcgaggtcg aggccttccg
     1801 cacgcccttc gcagagcacg gctacgacgc gcggcgggtg ttctatttcg gcgaatcgat
//
LOCUS       DAOTIL010000587         1857 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminobacteriaceae bacterium isolate AMR_MDS_3026
            NODE_74249_length_1857_cov_2.636515, whole genome shotgun sequence.
ACCESSION   DAOTIL010000587 DAOTIL010000000
VERSION     DAOTIL010000587.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868050
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminobacteriaceae bacterium (wastewater metagenome)
  ORGANISM  Aminobacteriaceae bacterium
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae.
REFERENCE   1  (bases 1 to 1857)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1857)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:19:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,224
            CDSs (total)                      :: 3,198
            Genes (coding)                    :: 3,184
            CDSs (with protein)               :: 3,184
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1857
                     /organism="Aminobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_74249_length_1857_cov_2.636515"
                     /isolate="AMR_MDS_3026"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:3034609"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(148..1254)
                     /locus_tag="P5201_11090"
     CDS             complement(148..1254)
                     /locus_tag="P5201_11090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014806212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LptF/LptG family permease"
                     /protein_id="HRV99131.1"
                     /translation="MRKRAGVGILDRFILKELSGSFLFGVTSFTMVFVAGDLLFQAAN
                     LIIEQGVSLGVVARLFIYRLPEVVMLTLPMASLLSSLLTFSRLSTNSELVALKSAGIP
                     FHRILRPVFLASILVSLASTIGAETVVPFANRAAENLMKFEILQEKPSLLKEKVFLRE
                     EKDGVLQRVLYLGELKPKDGLMKDIIIQEFEEGRLRRISLAGEGLWAGGEWWIENGQV
                     FEVTPAGKVQTLFRFERQRLHLNLSPVQIEQASRRPSEMSSLELLAQIALMEQQGQNL
                     APLKVMFHLRLALPWASVVLAVLGSSLGVRSTRAGPGIGFGLSVLIVFAYYVVMSFSR
                     ALGEAGYLPAFIASWFPNMSFLLVGGYFARRANG"
     gene            complement(1254..1763)
                     /locus_tag="P5201_11095"
     CDS             complement(1254..1763)
                     /locus_tag="P5201_11095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014162705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IIIA family hydrolase"
                     /protein_id="HRV99132.1"
                     /translation="MKRIKLLVLDVDGTLTDGGVYMDGRGGEFKRFDIHDGMGIVRLR
                     AAGVRTAFLSGRRSEATAQRAADLGVELLFNGVREKLPVLRSLLSDLSLSAEEAAYMG
                     DDVNDVDCLRAAGLAFAPADAVPEAKSAADFVTSAKGGGGAVREAAEHIISLNARCEG
                     RSSGAEERP"
BASE COUNT          414 a          616 c          507 g          320 t
ORIGIN      
        1 cccgcaataa gcgccccggc ggatttcccg gaaggtgaac acggcgaaac gtcctgtgtc
       61 atatattcca atcctttcac gatatcatct cttcccgccg ggacgctccg tttcagtgag
      121 cagtcccgcg gcgggacagg ctaagaatca gccgttcgct ctccttgcaa agtatccgcc
      181 gacgagaaga aacgacatgt tcggaaacca cgaggcgatg aacgccggaa ggtacccggc
      241 ctcgccgaga gcccggctga aggacatcac gacgtagtag gcgaaaacaa tgagcacgct
      301 gagtccgaac ccgattccgg gacctgcgcg cgtggagcgg acgccgagcg acgaccccag
      361 caccgcaagc acgacgctcg cccagggcag cgccagccgg aggtgaaaca tcactttcag
      421 cggcgcaagg ttctgcccct gctgctccat gagcgcgatc tgcgccagca gctccagaga
      481 gctcatttcc gaagggcgtc tcgacgcctg ctctatctgc acgggactca gattgagatg
      541 aaggcgctgc cgttcaaagc ggaagagcgt ctgcactttg ccggcaggcg ttacctcgaa
      601 cacctgaccg ttctctatcc accattcgcc ccctgcccac aacccctcgc cggccaggga
      661 gatcctccgc aaacgcccct cctcgaactc ctggataatg atgtctttca tgagaccgtc
      721 tttcggcttg agctctccga gatagagcac ccgctggagc acaccgtcct tttcttcccg
      781 aagaaagact ttttctttca gcagcgacgg tttctcctgc aggatctcga atttcatcag
      841 gttctccgcc gccctgttgg caaacggaac gacagtctcc gccccgatcg tcgaggcaag
      901 actcacgagg atggaggcaa ggaagactgg acgaagaatc ctgtgaaacg gtatccccgc
      961 cgacttcaag gcgaccagct cgctgttggt ggaaagcctg ctgaacgtca gcagcgacga
     1021 gagcagagag gccatgggga gcgtgagcat caccacctcg ggaagccggt agatgaagag
     1081 gcgggcaact acgccgagcg aaacgccctg ctcgatgatc aggttcgccg cctggaagag
     1141 cagatctccg gcgacaaaga ccatcgtgaa agaggtcacg ccgaaaagga aggagccgga
     1201 aagctccttc aggataaaac gatcaagaat accgaccccg gccctttttc tcatcacggt
     1261 ctctcttcag ctccggaaga acgcccctcg cagcgtgcat tcagcgaaat gatatgctcc
     1321 gccgcctcgc ggacagcccc gcctccgccc ttggcggacg tcacgaaatc cgccgcggac
     1381 ttcgcctccg ggactgcatc cgcaggagca aaagcaagac cggcagcccg gagacagtcg
     1441 acgtcgttca cgtcgtcccc catgtaggcc gcctcctctg cggaaagtga aagatccgac
     1501 agcagcgaac gcagcacggg aagtttctcc cttactccat taaaaaggag ctccacgccg
     1561 agatcggcgg cgcgctgcgc cgtcgcttcc gaacggcgtc cgctgaggaa cgccgtccgg
     1621 actcccgctg cgcgcagacg gacaatcccc attccgtcat ggatgtcgaa ccgcttgaac
     1681 tctccgccgc ggccgtccat atacactccc ccgtcggtga gcgtgccgtc gacatcgaga
     1741 acaagcaact taatcctctt catggtcctt cccccggaag gcatgtccct ttgtccagag
     1801 cgcaaccccc cgcttggatt ccccgcagaa ggagagaatc tccctggcaa ccggatc
//
LOCUS       DAOTIL010000588         1854 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminobacteriaceae bacterium isolate AMR_MDS_3026
            NODE_74404_length_1854_cov_3.367982, whole genome shotgun sequence.
ACCESSION   DAOTIL010000588 DAOTIL010000000
VERSION     DAOTIL010000588.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868050
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminobacteriaceae bacterium (wastewater metagenome)
  ORGANISM  Aminobacteriaceae bacterium
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae.
REFERENCE   1  (bases 1 to 1854)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1854)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:19:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,224
            CDSs (total)                      :: 3,198
            Genes (coding)                    :: 3,184
            CDSs (with protein)               :: 3,184
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1854
                     /organism="Aminobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_74404_length_1854_cov_3.367982"
                     /isolate="AMR_MDS_3026"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:3034609"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            109..1731
                     /locus_tag="P5201_11100"
     CDS             109..1731
                     /locus_tag="P5201_11100"
                     /inference="COORDINATES: protein motif:HMM:NF015983.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HRV99133.1"
                     /translation="MIGNLGILTLAAQLNADGFEAYAYTGITTDVVKTIGQMKERLFA
                     VCFYCDFDNQSAVEAIIKGLKNEAGNNFYVVLGGPHTLHMTERDLERYGADAIIKGEG
                     EESLPAWLRARRTGATVAVPGEILPGEPSEYVYLKDFSHYAPPQDEAIVGYGERPLMS
                     VITARGCPYRCAFCFEGGNSKNLRMRTPDDVLCEIEARLQKSRGPRYLFFCDDTFTAN
                     PERLSELLKGVRRLRERHDFVWFCEGHPGFLARRPELIAEMIASGMVRMQIGMESGDG
                     RVLQAYGKQAVPEDVKNVVDTCYREGLPQLAGNFIIGGAFETRRTLETTTGFALDLLH
                     RAPGMLDLSTTFLMPLPGTRISTHPGSFGIALEDRDCLTSIEDFPVNSTLELSVPEIC
                     RERSVFLTHISNAMKAQYANGRIPPERIRRDFELAFRYGIAAGYLKFIYGKDPLLVAY
                     YRKLTDYKGLLKEWNELEGAEKTTSFIQRVPDFSLVDFTVLSGAETDILLDAGSSSVK
                     ETAKKLNLSMEATSACLERLSAQHLTLFSPCL"
BASE COUNT          411 a          515 c          547 g          381 t
ORIGIN      
        1 gcattcgatg gaacagaaga aatggattct ctatcagaaa ccatgaacgc gtttttcctt
       61 gttctcctcg tgtacaccca acggctggtc ccggggagga cctttgaaat gatcgggaat
      121 ctgggaattc tcacgctcgc ggcgcagctc aacgccgacg gttttgaagc atacgcctat
      181 accggcatca caaccgatgt ggtcaaaacc attggacaaa tgaaagagcg ccttttcgcg
      241 gtctgtttct actgcgactt cgacaaccag tccgcagtgg aggcgatcat caagggtctg
      301 aaaaatgaag ccggaaataa tttctacgtt gtcctgggcg gaccgcatac gctgcacatg
      361 acggaacggg atctggaacg gtacggcgcg gacgccatca tcaaggggga aggggaagag
      421 tccctccccg cctggctccg ggcccgccgt acgggcgcaa cagtcgccgt gccgggggaa
      481 atacttcccg gggagccttc cgaatacgtg tatctgaagg atttttctca ctacgctccg
      541 ccgcaggatg aagcgatagt aggatacgga gaacggcccc tgatgtcggt gatcacggcc
      601 aggggttgtc cgtaccgctg cgcattctgt ttcgaggggg gcaattcgaa aaacttgcga
      661 atgcgcaccc cggacgacgt gctgtgtgaa atcgaagcgc ggttacagaa aagcaggggg
      721 ccacggtatc tgtttttttg cgacgacacc ttcaccgcca atcccgagcg cctgagtgag
      781 ctgctgaaag gggtgcggcg ccttcgggaa cgccatgatt tcgtctggtt ctgcgaaggg
      841 cacccgggtt ttttagcgcg ccgcccggag ctgatagccg aaatgatcgc gagcggtatg
      901 gtccggatgc agatcggcat ggagtccgga gatggtcgcg tccttcaggc atacgggaag
      961 caggccgttc cggaggacgt gaagaacgtg gtagatacct gctaccgcga ggggctgccc
     1021 cagctcgccg ggaatttcat catcgggggg gcttttgaaa cccggcgcac cctcgaaacg
     1081 accaccggct tcgctcttga tctgctgcat cgagcgccgg gaatgctgga tctctccacc
     1141 acttttctga tgccgctgcc gggaacgcga atttccacac atcccgggag cttcggcatc
     1201 gcccttgaag accgggactg tctgacgtcc atcgaggact ttccggtgaa cagtacgctc
     1261 gagctctccg ttccggaaat atgccgtgaa cggtcggtct ttctcacgca tatttccaac
     1321 gcgatgaagg cgcagtacgc gaacggccgg attcccccgg agcggatcag gcgcgacttc
     1381 gagctggcgt tccggtacgg catcgcggcg gggtacctga agttcatcta cggcaaggac
     1441 ccgcttctgg tcgcctacta tcggaaactt accgactata agggacttct gaaagaatgg
     1501 aacgaacttg aaggagcgga aaagacaacc tccttcatcc agcgcgttcc tgatttttcg
     1561 ctggtggatt ttacagtctt gagcggcgcg gaaacggata tcctgctgga cgccggttcc
     1621 tcttcggtga aggagacggc gaaaaaactg aatctgtcca tggaagcaac gagcgcctgc
     1681 ctcgaaagac tgagcgcgca gcatctgacg ctcttctcgc cctgcctgtg aaacggaatg
     1741 cgatgagagc gaacctgtcg tacacactac tctatggagg cccggagtga acactatcaa
     1801 cctgagactg atgctgattc atcgcggaac gagaacccat tcgatcaccc agat
//
LOCUS       DAOTIL010000589         1852 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminobacteriaceae bacterium isolate AMR_MDS_3026
            NODE_74513_length_1852_cov_3.494713, whole genome shotgun sequence.
ACCESSION   DAOTIL010000589 DAOTIL010000000
VERSION     DAOTIL010000589.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868050
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminobacteriaceae bacterium (wastewater metagenome)
  ORGANISM  Aminobacteriaceae bacterium
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae.
REFERENCE   1  (bases 1 to 1852)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1852)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:19:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,224
            CDSs (total)                      :: 3,198
            Genes (coding)                    :: 3,184
            CDSs (with protein)               :: 3,184
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1852
                     /organism="Aminobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_74513_length_1852_cov_3.494713"
                     /isolate="AMR_MDS_3026"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:3034609"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..493)
                     /locus_tag="P5201_11105"
     CDS             complement(<1..493)
                     /locus_tag="P5201_11105"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="RND transporter"
                     /protein_id="HRV99134.1"
                     /translation="MKSKLNRNGVNGKRIWKKLATTLAVCAVIGAAVFMLWPKPLEVE
                     TGLAERRPFTASVVEEGKTRIRHRYTVSSPVNGFLNRTALRPGDVVRAGETVLAVMEA
                     ESSGLLTPRLRAEAEARLRTAEAGRALRKSELDRAKVSLDLAKKQFERLDRLLPSGGI
                     SRQE"
     gene            complement(514..>1852)
                     /locus_tag="P5201_11110"
     CDS             complement(514..>1852)
                     /locus_tag="P5201_11110"
                     /inference="COORDINATES: protein motif:HMM:NF014717.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HRV99135.1"
                     /translation="LYQRFFNFPALIFRPDWSALLVALLASGLVSFLGVLGAVRQAAS
                     LPPAEAMRPEAPADYSPSFLEKAGLHRLVQPSFRIALRNLERKPWQAFFTALGLALAA
                     AIPVLPGAMADGVDYLVDFQWNMAMRQDAVVTFIEPGGGEALLSLSRFPGVLYAEPFR
                     AVPVRLFNGHRERTAAITGMPAAPRLNRLLDSQERVVPVPGSGLLLSEKLAEVLDLSP
                     GDEIRMEVREGRRPVLATFLSGTITDFTGVGAYMEIEGLRRLLGEGTTASGAYLQVDS
                     LYWDDFWRRVKETPRIGSVTTTAGARESFSKTTEEMMGVSQMLYLVFSLIVSFGVIYN
                     GARIALSERSRSLATLRVLGFTRREVTTILLSELGLLTLLSIGPGLLLGTWMAKSILE
                     SVDTETMRFPLILGARTYVVAVLTVFVSTVFSFVVVGRRIAGLDLLAVLKSAE"
BASE COUNT          382 a          615 c          550 g          305 t
ORIGIN      
        1 actcctgcct cgaaattccg cccgaaggaa gcagtctgtc caggcgctcg aactgcttct
       61 tcgcgagatc gaggctcacc ttcgcccggt cgagctccga cttccggaga gcgcgtccag
      121 cctccgcggt ccgcaaccgg gcttccgcct ccgcgcgaag ccgcggcgtc agcaggcccg
      181 aggattccgc ctccataacc gcaagaacgg tctcgcccgc acgaacgaca tctccggggc
      241 gcagcgccgt acggttcaga aagccgttca caggggagga gaccgtgtac cggtgtcgta
      301 tcctcgtctt tccttcctcc acgacgctcg cggtgaaggg cctgcgctcc gcaagccccg
      361 tctccacttc cagaggtttc ggccacagca tgaagacggc cgcgccgatg acggcgcaca
      421 ctgcaagagt tgtcgcgagt tttttccaga ttctttttcc gttgacgccg tttctgttca
      481 gtttcgactt caccaactcc accccgtttc gcgctattca gcggatttca gcaccgcgag
      541 cagatcgagc ccggcgatcc gtcgtcccac cacgacgaaa gaaaaaaccg tggacacaaa
      601 cacggtcaac actgcgacga catacgtcct cgctccgagt atcagaggga agcgcatggt
      661 ctcggtgtcc accgactcta gaatggattt cgccatccac gtccccagca ggagcccggg
      721 accaatcgaa agaagcgtga gaagccccag ttccgaaagg agaatggtcg tcacttcgcg
      781 gcgggtgaac ccgagcacgc gcagcgtcgc cagcgagcgg gagcgctccg aaagagcgat
      841 gcgcgccccg ttgtagatca ctccgaagga gacgatgagg gaaaagacaa gatagagcat
      901 ctgcgacacg cccatcatct cctcggtggt cttgctgaaa ctctccctgg cgcctgcggt
      961 cgtggtcacc gaaccgatgc ggggagtttc tttcaccctc cgccaaaaat catcccagta
     1021 cagcgagtcc acctgcaggt aagcgccgct tgctgtcgta ccctctccaa gaagcctgcg
     1081 cagtccttcg atctccatat aggcgcccac cccggtaaag tcggtgatag ttccggagag
     1141 gaaggttgcg agcacgggac ggcgcccttc tcgcacttcc attcggatct cgtcgccggg
     1201 ggagaggtcc agcacttcgg cgagtttctc ggagagcagc agcccggaac cgggtacggg
     1261 gacaacccgt tcctgcgagt ccagaaggcg gttgagacgg ggcgccgccg gcattccggt
     1321 gatcgccgcc gtccgctccc tgtgtccgtt gaacagcctg acaggaaccg cccggaacgg
     1381 ctcggcgtag agaacccccg gaaagcgcga caacgagagc agcgcctcac ccccgcccgg
     1441 ttcgataaac gtgacgaccg cgtcctgacg catcgccatg ttccattgaa agtccacaag
     1501 gtagtcgacg ccgtcggcca tcgctcccgg aagcacggga atggccgcgg cgagcgccag
     1561 cccgagagcg gtgaaaaaag cctgccacgg ttttcgttcc agattgcgca gcgcgatcct
     1621 gaaggagggt tgcacgagtc tgtggagtcc ggccttctcc agaaaggacg gggagtaatc
     1681 cgcgggagcc tccggccgca tcgcctccgc gggagggagc gacgccgcct gcctgaccgc
     1741 gcccagcacc ccgaggaacg ataccaaccc gctcgccagc agcgcgacga gcaaagcgct
     1801 ccagtcggga cggaagatca acgcagggaa gttgaaaaag cgctggtaga gc
//
LOCUS       DAOTIL010000590         1852 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminobacteriaceae bacterium isolate AMR_MDS_3026
            NODE_74516_length_1852_cov_3.420701, whole genome shotgun sequence.
ACCESSION   DAOTIL010000590 DAOTIL010000000
VERSION     DAOTIL010000590.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868050
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminobacteriaceae bacterium (wastewater metagenome)
  ORGANISM  Aminobacteriaceae bacterium
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae.
REFERENCE   1  (bases 1 to 1852)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1852)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:19:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,224
            CDSs (total)                      :: 3,198
            Genes (coding)                    :: 3,184
            CDSs (with protein)               :: 3,184
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1852
                     /organism="Aminobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_74516_length_1852_cov_3.420701"
                     /isolate="AMR_MDS_3026"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:3034609"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..324)
                     /locus_tag="P5201_11115"
     CDS             complement(<1..324)
                     /locus_tag="P5201_11115"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRV99136.1"
                     /translation="MSLLSGGMGWRRKGGTKMRAHSRLAAATVAVSMLVGAGGGIASA
                     DANSSLAESMNLAPRRNADGNYNIIFITTDQERYIKDFPAGTAYEARELLASLGATFE
                     KHYTCA"
     gene            complement(346..>1852)
                     /locus_tag="P5201_11120"
     CDS             complement(346..>1852)
                     /locus_tag="P5201_11120"
                     /inference="COORDINATES: protein motif:HMM:NF012318.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="trypsin-like peptidase domain-containing
                     protein"
                     /protein_id="HRV99137.1"
                     /translation="KASVVRKEYDDETGGNDFALLRFSSSAKLPVLSFTLRVNRMDRV
                     SAWGFPVLVTQFDQSFDDILDGKRTKTPPVVYTEGTVSAVVERRGQKTVIHSAAIAGG
                     NSGGPLVNARGEVVGINTWGYTEEDEGAFVNASIPSERIVDFLLKAGVKPQMSRQSPE
                     LARTPLPVPLPAPLPEEPKKPAPSVELPAPPMEDDDGNEELFRMARAGDVDAQAALGA
                     MYYDGDGVPQDTKQAVQWLEKAVKGGSEEGKALLGVMLVFEEDVADPKKGVRLLREAA
                     SSVDEDGWISGLLGRIYYEGEIYGVPRDLDESFRWAGKAAEGGDPDGTALLGMLYYFG
                     EGTDEDDELALELTEQAVEEDSSLGKAILAWMYYDGVAVDEDLETALALATDAAEEEE
                     ALAQGLLALMYAEGHGVERDVKSAEGWARRAADQGNEFGWFVLGSLYMRGEVVKKDPA
                     MAWAYLDLAAKRSVANADERLEELEKTISSRDKKRAQELQKQWRKEWGLSR"
BASE COUNT          274 a          684 c          534 g          360 t
ORIGIN      
        1 ggcgcaggta tagtgcttct cgaaggtggc gccgagcgag gccagcagct cccgcgcctc
       61 gtaggccgta ccggcgggga agtccttgat gtagcgttcc tggtcggtgg tgatgaagat
      121 gatgttgtag ttcccgtcgg cgttccgtct cggagcaagg ttcatactct ccgcaagcga
      181 agaattcgca tccgcggagg ctatgccgcc tcccgcgccc acgagcatgg aaacggcaac
      241 agtagcggcc gccaatctag agtgtgcacg catcttcgtt cctccctttc tacgccatcc
      301 catgccgccg gacagcaacg acatgccccg gcctccgcgc cctgttcagc gactcaggcc
      361 ccactccttc cgccactgct tctgcagctc ctgcgcccgt ttcttgtcgc gggaggagat
      421 ggtcttttcc agctcttcga gccgttcgtc ggcgttcgcc acggagcgct tcgccgccag
      481 gtccaggtag gcccacgcca tggcggggtc cttcttcaca acctcgccgc gcatgtacag
      541 ggatcccagg acgaaccagc cgaactcgtt cccctggtcc gccgccctcc gcgcccatcc
      601 ttcggcgctc ttcacatcgc gctccacccc atgtccctcg gcgtacatca gcgcgaggag
      661 cccctgcgcc agagcctctt cctcctcggc cgcgtccgtc gccagcgcca gggccgtctc
      721 aagatcctcg tcgaccgcga cgccgtcgta gtacatccac gcgaggatcg cctttccgag
      781 cgagctgtcc tcctcgaccg cctgctccgt cagctccaga gcgagctcgt cgtcctcgtc
      841 cgtcccttcg ccgaagtagt agagcatccc cagcagcgct gtgccgtccg ggtctcctcc
      901 ctcggcggcc tttccggccc accggaaact ctcgtcgagg tcccggggca cgccgtagat
      961 ctccccctcg tagtaaatgc gcccgagcag tccggaaatc cagccgtcct cgtccacgga
     1021 agaggccgct tcgcggagca ggcggacgcc cttcttcggg tcggcgacat cctcctcgaa
     1081 gacgagcatg acgccgagca gcgcttttcc ctcctcgctc ccgcccttta ccgccttctc
     1141 cagccactgg acggcctgct tcgtgtcctg cggcacgccg tctccgtcgt agtacatcgc
     1201 ccccagggcg gcctgcgcgt cgacgtctcc cgccctcgcc atacggaaca actcctcgtt
     1261 cccgtcgtcg tcttccatcg ggggcgcggg gagctcgacg gacggggcgg gcttcttcgg
     1321 ttcctctgga agaggtgcgg gaagcggcac aggaagcggc gtccgggcga gttccggcga
     1381 ctggcgcgac atctgcggtt tgacgcccgc tttcagcagg aagtcgacga tccgctccga
     1441 cggtatggag gcgttcacga acgcgccttc gtcctcctcc gtataccccc atgtattgat
     1501 cccgacgacc tcgccgcgcg cgttcaccag agggccgccg ctgtttcccc ctgcgatggc
     1561 cgcggagtgg atgacggttt tctgccctct ccgctcgacc actgcgctca ccgtgccctc
     1621 ggtgtacacc accggagggg ttttggtccg ctttccgtcg agaatatcgt cgaagctctg
     1681 gtcgaattgg gtgacgagca ccgggaaccc ccacgcgctc accctgtcca tcctgttcac
     1741 ccgcagcgta aaggagagca caggcagctt cgcagaggag gagaatcgca gcagggcgaa
     1801 gtcgttccct cccgtctcgt cgtcgtactc cttccgcacg acggaggcct tc
//
LOCUS       DAOTIL010000591         1850 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminobacteriaceae bacterium isolate AMR_MDS_3026
            NODE_74633_length_1850_cov_4.062953, whole genome shotgun sequence.
ACCESSION   DAOTIL010000591 DAOTIL010000000
VERSION     DAOTIL010000591.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868050
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminobacteriaceae bacterium (wastewater metagenome)
  ORGANISM  Aminobacteriaceae bacterium
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae.
REFERENCE   1  (bases 1 to 1850)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1850)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:19:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,224
            CDSs (total)                      :: 3,198
            Genes (coding)                    :: 3,184
            CDSs (with protein)               :: 3,184
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1850
                     /organism="Aminobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_74633_length_1850_cov_4.062953"
                     /isolate="AMR_MDS_3026"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:3034609"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..210)
                     /locus_tag="P5201_11125"
     CDS             complement(<1..210)
                     /locus_tag="P5201_11125"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRV99138.1"
                     /translation="MTSPLDARETRWRKRIDLASALPPGCSLLSFTLRMPAPLRLGGE
                     YDRTAGEWFDELRQFLAKEGMSAPSY"
     gene            complement(210..479)
                     /gene="citD"
                     /locus_tag="P5201_11130"
     CDS             complement(210..479)
                     /gene="citD"
                     /locus_tag="P5201_11130"
                     /EC_number="4.1.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006299618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009346 - ATP-independent citrate lyase
                     complex [Evidence IEA];
                     GO_function: GO:0008815 - citrate (pro-3S)-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0006113 - fermentation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="citrate lyase acyl carrier protein"
                     /protein_id="HRV99139.1"
                     /translation="MNGVKNSVAGSLESSDVLVTVGPAEGAENTLDVESIVFKQFGAR
                     IRSVAEEVMAASGVRGADVRIQDRGALECTLRARLETALERALKA"
     gene            complement(518..1597)
                     /locus_tag="P5201_11135"
     CDS             complement(518..1597)
                     /locus_tag="P5201_11135"
                     /inference="COORDINATES: protein motif:HMM:NF019824.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="[citrate (pro-3S)-lyase] ligase"
                     /protein_id="HRV99140.1"
                     /translation="MFGLEERALVSPREMEERSRLLASRGLTVPGNESVILGFFEEER
                     LVATGSLVGNILQGIAVDRELEGEGVAATVTSALLKKAMQMGRRQLFLYSKPDEAKRF
                     EALGFTLLATASVERTGLGASLLEWGSEGIDKWRAAIATKAEGGPEGAGVAVVNCNPF
                     TLGHYCLLEYAAARTPRLYVLVVEEDRSLFPFEVRFRLVKEGVSELKNVVALRGGPYV
                     ISSATFPSYFTRIESGEEERITELYAALDIELFGRYVASALKATERFVGTEPYCPVTS
                     IYNRMMKERLPKGGEGWPPVRVHEMPRFEMEGLPVSASRVRSLIREDRMEDVRPLVPE
                     STWKWLVSDEALPVIEKIRNSESRH"
     gene            complement(1619..>1850)
                     /locus_tag="P5201_11140"
     CDS             complement(1619..>1850)
                     /locus_tag="P5201_11140"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRV99141.1"
                     /translation="LPLLFEDRADLEKIAQGDRLELVNLHSCLEGEFVEVRNVTTGAC
                     IRTRHGLSRRQREILLAGGALNAARVALTKEP"
BASE COUNT          334 a          673 c          511 g          332 t
ORIGIN      
        1 atatgagggc gcactcatcc cctccttcgc cagaaactgc cgaagctcgt cgaaccactc
       61 cccggcagtc cggtcgtact cgccgccgag gcgcagcggc gcgggcatcc tgagcgtgaa
      121 ggagagcagc gaacagccgg gcgggagcgc ggaggcaaga tcaatccgct tccgccaccg
      181 cgtttccctg gcgtcgagag gggacgtcat caggccttga gcgcccgctc cagagccgtc
      241 tccagacggg cgcggagcgt gcactccagc gctcccctgt cctgaatccg cacatctgcg
      301 cccctgacgc ccgaggcggc catgacctcc tcggcgacag agcggatacg cgctccgaac
      361 tgtttgaaga cgatgctctc cacgtcgagt gtgttctccg ccccctccgc ggggcctacc
      421 gtcaccagca cgtcgcttga ttcgagcgac cccgcgacgc tgttcttcac accgttcata
      481 caaccctctc ctttcggcgg actcgaagcg tccgtattca atgtctcgac tcgctgttcc
      541 tgattttttc gatcacaggg agcgcttcat ccgaaacgag ccacttccac gtggactcgg
      601 gaacaagcgg cctgacgtct tccatacggt cctctcggat cagggagcgc actcgcgacg
      661 cgctgacggg caggccctcc atctcgaaac gcggcatctc atggacgcgc acgggcggcc
      721 atccctcgcc gcccttcggc aggcgctcct tcatcattct attgtagatg gaggtcacgg
      781 ggcaatacgg ctccgtcccc acgaaacgct ccgtcgcctt gagcgccgaa gcgacgtacc
      841 gtccgaagag ttcgatgtcg agcgcagcgt agagctccgt aatccgttcc tcctcgccgg
      901 attcgatccg cgtgaaatac gacgggaagg tcgccgacga gatgacgtac ggcccgccgc
      961 gaagcgctac gacgttcttc agctcggaca ctccctcctt gacaaggcgg aagcggactt
     1021 cgaaggggaa gagcgaccgg tcctcctcca cgacgaggac atagagccgc ggcgttcgtg
     1081 cggcggcata ctccagcagg cagtagtgcc ccagcgtaaa cgggttgcag ttcaccaccg
     1141 cgacgccggc cccctccggg ccgccctccg ccttcgtggc tatcgccgct ctccatttgt
     1201 ctattccttc ggagccccac tccagcagcg aagctccaag acccgtccgc tccaccgatg
     1261 cagtcgccag cagcgtgaag ccgagcgcct cgaagcgctt cgcctcgtcc ggtttcgagt
     1321 acaggaagag ctgcctgcgc cccatctgca tcgctttttt cagcagagcg gaggtcaccg
     1381 tcgcggcgac gccctccccc tcaagttccc gatcgaccgc gatcccctgc agaatattcc
     1441 ccaccaggga gccagtcgcg acaagacgtt cttcctcgaa aaatcccagg atcacggatt
     1501 cgtttcccgg aaccgtgagg cccctcgacg ccagcaatcg cgagcgttcc tccatctccc
     1561 tcggcgagac cagcgcccgc tcctccagtc cgaacatgcg cgctccccct ttcgaccgtt
     1621 acggttcttt cgtcagcgca acgcgggcgg cgttgagcgc cccgccggcg agcagaatct
     1681 cccgctgtct ccgcgaaagc ccgtggcgcg tcctgataca agctcccgtc gtcacattcc
     1741 gcacctcgac aaactcccct tcgaggcaag agtgcagatt taccagctcc agccggtccc
     1801 cttgcgcgat cttctcaaga tcggccctgt cttcgaagag aagcggaaga
//
LOCUS       DAOTIL010000592         1849 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminobacteriaceae bacterium isolate AMR_MDS_3026
            NODE_74691_length_1849_cov_3.523969, whole genome shotgun sequence.
ACCESSION   DAOTIL010000592 DAOTIL010000000
VERSION     DAOTIL010000592.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868050
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminobacteriaceae bacterium (wastewater metagenome)
  ORGANISM  Aminobacteriaceae bacterium
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae.
REFERENCE   1  (bases 1 to 1849)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1849)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:19:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,224
            CDSs (total)                      :: 3,198
            Genes (coding)                    :: 3,184
            CDSs (with protein)               :: 3,184
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1849
                     /organism="Aminobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_74691_length_1849_cov_3.523969"
                     /isolate="AMR_MDS_3026"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:3034609"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..217
                     /locus_tag="P5201_11145"
     CDS             <1..217
                     /locus_tag="P5201_11145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009165277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="2-oxo acid dehydrogenase subunit E2"
                     /protein_id="HRV99142.1"
                     /translation="FFTPVINQPESAILGVGRTNDMPVAVDGEVAVRPMTALSLAHDH
                     RVIDGAPAAGFLALLIELIEHPFRVFL"
     gene            276..785
                     /locus_tag="P5201_11150"
     CDS             276..785
                     /locus_tag="P5201_11150"
                     /inference="COORDINATES: protein motif:HMM:NF012514.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HRV99143.1"
                     /translation="MESWKGCAYTALIVFGYLVRGNKVLIVRRANDPYKGQRTVPGGR
                     KQRDETLREALVREIEEETGCRVRSMEYAGMLHAIVEGRDMEYLSVYFISRDFDGEPR
                     DSEEGTVEWMDIDESLTMEDMHPIYLRLVPYILKGNYPMEGAMRISADDASEYMLFGP
                     GEHPAPEAR"
     gene            complement(863..1771)
                     /locus_tag="P5201_11155"
     CDS             complement(863..1771)
                     /locus_tag="P5201_11155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006301946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exopolyphosphatase"
                     /protein_id="HRV99144.1"
                     /translation="MRLITRSDFDGLICGMLLKEAGIIDSWQFVHPKDLQDGLFQPTE
                     NDVLANVPYVPGCGMWFDHHTSENDRVGWSKDVQGESRLAPSAARIIYEYYGGAEKFP
                     AYGDMIEAVDKVDSGQLTREEILNPKGWILLGFLSDPRTGLGRFREFRISNYQLMEEL
                     INHCRNMRIGEILEQPDVKERVELYFEQTRRFTEMVHTHTKVEQNVIVTDLRDVSPIY
                     TGNRFLIYSLYPEQNVSVWIVDGRQKQNVSIACGYSILNRTCGVDIGALMLKHGGGGH
                     KMVGTCQVPYSEADGRIREIVDALKG"
BASE COUNT          326 a          560 c          613 g          350 t
ORIGIN      
        1 gttcttcacg cccgtcatca accagccgga atcggccatc ctcggggtgg gcaggacgaa
       61 cgacatgccc gtcgcagtgg acggagaggt cgcggtgcga ccgatgacgg cgctctcgct
      121 ggcgcacgac cacagggtca tcgacggcgc gccggcggcg gggttcctcg ccctgttgat
      181 cgagctgatc gaacatccgt tccgggtctt cctctgaggt gtatgataac aggagtcgcc
      241 ggcgacgccg ggaatacatg gaaagagggt gcggcatgga atcgtggaag gggtgcgcgt
      301 ataccgcgct gattgtcttc ggatacctgg tgcgggggaa caaggtgctc atcgtccgcc
      361 gggccaacga cccgtacaag gggcagcgca ccgtccccgg ggggcggaag cagcgggatg
      421 agactctgcg cgaggcgctg gtgcgcgaaa tagaggaaga gacggggtgc agggtgcgga
      481 gcatggagta cgccgggatg ctccacgcga tcgtcgaggg gcgggatatg gagtacctct
      541 cggtctactt catctcgcgc gacttcgatg gggagccccg cgacagcgag gaggggaccg
      601 tcgaatggat ggatatcgac gagagcctca cgatggagga catgcacccg atctaccttc
      661 gccttgtgcc gtacatcctg aagggaaact acccgatgga gggcgcgatg cggatctccg
      721 cggacgacgc ttcggagtac atgctcttcg ggccggggga gcaccccgcg ccggaagcgc
      781 ggtagacggg gcggagcaga gcgcggacag cgccgggaag agtacagggg gagctcccgt
      841 gcggggctcc ccctgattcg tctcatccct tgagcgcgtc cacgatctcg cggatgcggc
      901 cgtccgcctc gctgtacggt acctggcaag tgccgaccat tttgtgaccg ccgcctccgt
      961 gcttcagcat gagcgcgccg atgtccaccc cgcacgtgcg gttcagaatg ctgtatccgc
     1021 aggcgatgga gacgttctgc ttctgcctgc cgtcgacgat ccagacggag acgttctgct
     1081 ccgggtagag actgtagatg aggaagcggt tgcccgtgta gatgggggag acgtccctca
     1141 ggtcggtgac gatgacgttc tgttccacct tcgtgtgtgt atggaccatc tccgtgaaac
     1201 gccgcgtctg ctcgaagtag agctccactc gctccttcac gtccggctgt tcgaggatct
     1261 cgccgatgcg catgttccgg cagtggttga tcagctcctc catgagctgg tagttgctga
     1321 tgcggaactc gcggaatctg ccgagccccg ttctggggtc ggagaggaag ccgaggagga
     1381 tccacccctt cggattcagt atctcctccc gggtaagctg ccccgaatcc accttgtcca
     1441 ctgcctcgat catgtcgccg taggcgggaa acttctccgc gccgccgtag tattcgtaga
     1501 tgatccgcgc cgcggaaggc gcgagtcggc tctcgccctg cacgtccttc gaccagccga
     1561 cgcggtcgtt ctccgacgta tggtggtcga accacatccc gcagccgggg acgtacggaa
     1621 cgttcgccag cacgtcgttc tccgtcggct gaaagagtcc gtcctgaaga tccttcgggt
     1681 ggacgaactg ccacgagtcg atgatccccg cctccttgag cagcataccg cagatcaggc
     1741 cgtcgaaatc agagcgtgtg atcaatcgca tgtcctacca actccccgtc tatttttcgc
     1801 ttgcagagcg ttctccatct cttgggcgga tcccgggcgg gaccgccgt
//
LOCUS       DAOTIL010000593         1849 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminobacteriaceae bacterium isolate AMR_MDS_3026
            NODE_74707_length_1849_cov_2.664437, whole genome shotgun sequence.
ACCESSION   DAOTIL010000593 DAOTIL010000000
VERSION     DAOTIL010000593.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868050
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminobacteriaceae bacterium (wastewater metagenome)
  ORGANISM  Aminobacteriaceae bacterium
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae.
REFERENCE   1  (bases 1 to 1849)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1849)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:19:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,224
            CDSs (total)                      :: 3,198
            Genes (coding)                    :: 3,184
            CDSs (with protein)               :: 3,184
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1849
                     /organism="Aminobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_74707_length_1849_cov_2.664437"
                     /isolate="AMR_MDS_3026"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:3034609"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..858)
                     /locus_tag="P5201_11160"
     CDS             complement(<1..858)
                     /locus_tag="P5201_11160"
                     /inference="COORDINATES: protein motif:HMM:NF017181.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase"
                     /protein_id="HRV99145.1"
                     /translation="MKIDMQYVMKLTEELIGIPSVAGDCREILARVERECVSLGLSVE
                     RSRKGDLLATLKGERDDGKRMVSAHVDTLGAIVREIKPNGRLRLLQIGGFAWTSFEAE
                     NLIVRTSSGREIRGSLLPEKASVHAFSDEVRTTLRTDENVEARLDENVSSAEETRALG
                     VEVGDFVFFDPRYEVTSSGFVKSRFLDDKLCVACIFGALKALVESKRLPARTTVLYIS
                     QYEEVGHGISAMPKDVEEHLAVDVGIVAPPANSREDAVTIVARDSRTPYDFEFRKFLS
                     DLAVKNSVPF"
     gene            complement(880..1842)
                     /locus_tag="P5201_11165"
     CDS             complement(880..1842)
                     /locus_tag="P5201_11165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013049036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HRV99146.1"
                     /translation="MVEVNNLKKYFHTKKGMLHAVDDVSFAINKGETLGLVGESGCGK
                     STLGRVMIRLLEATSGEVKFWGNDILKYSSSKMKEMRKRVQIVFQDPYSSLNPRLAVS
                     ELIAEPLLVNKVFRSASERSKKILELMDTVGLAKRLVNSYPHELDGGRRQRIGIARSL
                     ALSPEFIVLDEPVSALDVCIQAQVLNLLNSLQKQFGYTYVFISHNLSVVKHVSDRIAV
                     MYLGKIVEMSDYQSLFKEPLHPYTQALLSAIPIARVGQKRDRLILEGDVPSPIEPPEG
                     CRFQGRCFYRQDICKEKTPELRELEPGRSVACHFARQLVRTNGR"
BASE COUNT          377 a          556 c          528 g          388 t
ORIGIN      
        1 gaacggcaca gaattcttta cggcgaggtc tgaaaggaac ttccggaact cgaaatcgta
       61 cggcgtccgg ctgtctctgg cgacgatagt gaccgcatct tctctggagt tagcgggagg
      121 agccacgatg ccgacgtcta cggccagatg ctcctcgaca tccttgggca ttgcggaaat
      181 gccgtgcccg acctcctcat actgcgaaat gtacaggacg gtcgtccgcg caggaaggcg
      241 tttcgactcc acaagcgcct tgagggcgcc gaagatgcac gccacgcaga gcttgtcgtc
      301 gaggaagcgc gatttgacga atccggaaga ggtgacttcg tagcgaggat cgaagaagac
      361 gaagtcgccg acctccacgc caagcgcccg agtctcctcg gctgaagaga cgttctcgtc
      421 caggcgcgcc tcgacgtttt catccgtccg gagcgtcgtg cgcacctcgt cggaaaaggc
      481 atgcaccgag gccttctccg gaagaagcga tccgcggatt tcccggccgg aagatgtgcg
      541 cacgatcaga ttctccgctt cgaaggaggt ccatgcgaat ccccctatct gcaggaggcg
      601 cagacggccg ttcggtttga tctccctgac gatggctccg agagtgtcca catgcgcgga
      661 aaccatcctc ttgccgtcgt cccgctcgcc cttcagcgta gccagaagat cccccttgcg
      721 cgacctctcg acggagaggc cgagcgagac gcattcccgc tcaacccgcg ccagaatctc
      781 gcggcagtcg cccgcgaccg acggaattcc gatgagttcc tccgtcagtt tcattacata
      841 ctgcatatct atcttcatcg agtgagagcg tcctttcttt tatctgccat ttgtccttac
      901 gagctggcgg gcaaagtggc acgccaccga acgccccggt tcaagctccc tgagttcggg
      961 cgtcttctct ttgcagatat cctggcggta gaagcagcgt ccctggaaac ggcaaccttc
     1021 cggcggttcg atagggctgg gaacgtcgcc ttcaagaatc aacctgtcgc gtttttgacc
     1081 gactctcgcg atcgggattg ccgaaagaag cgcctgcgtg tacgggtgca gcggctcctt
     1141 gaagaggctc tgatagtcgg acatttcgac gatcttgccg agatacatca ccgcgatcct
     1201 gtccgaaacg tgcttcacca cgctcaagtt atgcgagatg aagacgtatg tgtagccgaa
     1261 ctgcttctgc aggctgttca gcaggttgag cacctgcgcc tggatgcaga cgtcaagggc
     1321 cgacaccggt tcgtcgagga cgatgaactc gggtgaaagc gcaagcgagc gggcgatacc
     1381 gatacgctgg cggcgccccc cgtcgagctc gtgggggtat gagtttacaa gtcgttttgc
     1441 cagcccgaca gtgtccatca attccaggat cttcttgctc cgctcggagg cggaccggaa
     1501 aaccttgttc accagcagag gttccgcgat aagttccgag acagcgagcc tgggattcag
     1561 acttgaatac gggtcctgga agacgatctg cacgcgcttg cgcatctcct tcatcttcga
     1621 ggaagaatac ttgagaatgt catttcccca aaacttcacc tcgccgctcg tcgcttccag
     1681 gaggcggatc attacgcgcc caagcgtgga cttgccgcag ccagactcgc cgacgagacc
     1741 gagcgtctct cccttgttga tagcgaacga gacgtcgtcc acagcgtgga gcatcccctt
     1801 cttggtgtgg aagtacttct tcagattgtt gacctcgacg atcgtcttc
//
LOCUS       DAOTIL010000594         1847 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminobacteriaceae bacterium isolate AMR_MDS_3026
            NODE_74787_length_1847_cov_3.843192, whole genome shotgun sequence.
ACCESSION   DAOTIL010000594 DAOTIL010000000
VERSION     DAOTIL010000594.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868050
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminobacteriaceae bacterium (wastewater metagenome)
  ORGANISM  Aminobacteriaceae bacterium
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae.
REFERENCE   1  (bases 1 to 1847)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1847)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:19:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,224
            CDSs (total)                      :: 3,198
            Genes (coding)                    :: 3,184
            CDSs (with protein)               :: 3,184
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1847
                     /organism="Aminobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_74787_length_1847_cov_3.843192"
                     /isolate="AMR_MDS_3026"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:3034609"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..204)
                     /locus_tag="P5201_11170"
     CDS             complement(<1..204)
                     /locus_tag="P5201_11170"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRV99147.1"
                     /translation="MNCENSVVVSGAIHLPKGDMVGRNRHGTYLKFALKQMTTGYDGV
                     ERPSFLPVRVFDQALQEQFRTKGH"
     gene            complement(295..1200)
                     /locus_tag="P5201_11175"
     CDS             complement(295..1200)
                     /locus_tag="P5201_11175"
                     /inference="COORDINATES: protein motif:HMM:NF015995.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine ABC transporter
                     substrate-binding protein"
                     /protein_id="HRV99148.1"
                     /translation="MKKLLALFLAIVLMTGFVAAANAADDPMKYEGRLSIASQNVNET
                     IILAWMAKLLVEEYTGLRTTMNTEFAGSAVLHQAMAAGEIDLYSSWTGTQLTGILRYE
                     GESKSSEETFRMVKEGFEKNFGMTWTRPVGFNNTYIMAVRPETAEKYNLKKASDLAAH
                     APGWKLGGDENFDTRLDAYPGWSERYGITFRDVLPMQYAMMYMALMQEEVDVIAAYST
                     DSRIGKFNLVLLEDDKQFFPDYSAAFVLPLALTEKYPGLVGVVEKLSGKIDEKAMAAM
                     NLAFDEGENPEDIARAFLREKQLVK"
     gene            complement(1257..>1847)
                     /locus_tag="P5201_11180"
     CDS             complement(1257..>1847)
                     /locus_tag="P5201_11180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014163920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HRV99149.1"
                     /translation="TVCVPLGIYLTRNERAAPYVIGLANVFQTVPSLALLGFLIFVLG
                     IGNDNAICALFLYAMLPVLQNTYTGIRGVPKSYVQAARGMGMTDFQILMKVQFPLALP
                     VLVAGIRVAMVWTIGTATLASAIGGGGLGRLIFSGLSSIRNEIILAGAIPATLLALAA
                     DWLLKRAQEYFNPSARAKRLAKRHGISAETESNSAA"
BASE COUNT          357 a          569 c          543 g          378 t
ORIGIN      
        1 gtgccccttg gtccgaaact gctcctggag cgcctgatcg aaaacgcgca ccgggagaaa
       61 gcttggccgt tccacaccgt catacccggt cgtcatctgc ttgagggcga acttaaggta
      121 cgttccgtgc ctgttgcgcc cgaccatgtc gcccttgggc agatgaatcg cgccggaaac
      181 tacaacggaa ttttcacagt tcaactacaa tccaactcct taaaaaccaa tcttcctacc
      241 cctgtgagta gatgaagaag ataccacttt ttcccctttt ccgcaaattt ttttctactt
      301 gacgagttgt ttttcacgga ggaaggcgcg tgcgatgtcc tcggggtttt ctccctcgtc
      361 gaaggccaga ttcatggcgg ccatcgcctt ctcgtcgatc ttcccgctca gcttctcaac
      421 gaccccgaca agcccggggt acttctccgt aagcgccaga ggcagaacga acgcggcgct
      481 gtagtcgggg aagaactgct tgtcgtcttc cagaagaacg aggttgaact ttcctatccg
      541 cgaatcggtc gagtaggccg cgatgacgtc gacttcttcc tgcatcagcg ccatgtacat
      601 catggcgtac tgcatcggca ggacgtcgcg gaaggtgatg ccgtagcgct cggaccagcc
      661 gggataggcg tcgagacgcg tgtcgaagtt ctcgtcgccg ccgagcttcc acccgggagc
      721 gtgcgccgcc agatcggacg ccttcttcag gttgtacttc tccgccgtct cggggcgcac
      781 ggccatgatg tacgtgttgt tgaagccgac cgggcgcgtc cacgtcatgc cgaagttctt
      841 ctcgaacccc tctttcacca tccggaaggt ctcttcggag gacttcgatt cgccctcgta
      901 gcgcagaatg ccggtgagct gcgtcccggt ccacgaggaa tagaggtcga tctccccggc
      961 ggccatggcc tggtgaagca ccgccgaccc ggcgaactcc gtgttcatcg tcgtgcgcag
     1021 cccggtgtac tcctcgacga ggagcttcgc catccaagcg aggatgatcg tctcgttgac
     1081 gttctgcgac gcgatactca gccgcccctc gtacttcatc ggatcatcgg ccgcgttggc
     1141 ggcggcgaca aaccctgtca tcagtactat cgcaagaaat agtgcaagca acttcttcat
     1201 ggaaccactc tcctttaaga tattttttac atcgttcaca gcgcggagcg cggagctcac
     1261 gccgcggagt tggattctgt ctcggcggaa ataccgtgcc tcttcgccag ccgcttcgcc
     1321 cgcgcggagg ggttgaaata ctcctgcgcg cgcttcagca gccagtccgc ggcgagcgcg
     1381 agcagcgtcg cggggatggc cccggcgagg atgatctcgt tccgtatgga agagaggccg
     1441 gagaagatga gccgtccgag tccgccgccg ccgatcgccg aggcgagcgt cgccgtgccg
     1501 atcgtccaga ccatggccac ccggatgccc gcgacgagca cgggaagggc gagtgggaac
     1561 tgtaccttca tcaggatctg gaagtcggtc atccccatgc cgcgcgcggc ctgaacgtag
     1621 gacttcggaa cgcctctgat gccggtgtag gtgttctgga gcaccggaag catggcgtag
     1681 aggaagagcg cgcagatcgc gttgtcattg cctattccca gaacgaagat caggaaaccc
     1741 aacagggcaa ggctcggcac cgtctgaaag acgttcgcca ggccgatcac gtacggggct
     1801 gcgcgctcgt tccgcgtgag gtagatcccc agtggaacgc agacggt
//
LOCUS       DAOTIL010000595         1843 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminobacteriaceae bacterium isolate AMR_MDS_3026
            NODE_74988_length_1843_cov_4.298098, whole genome shotgun sequence.
ACCESSION   DAOTIL010000595 DAOTIL010000000
VERSION     DAOTIL010000595.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868050
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminobacteriaceae bacterium (wastewater metagenome)
  ORGANISM  Aminobacteriaceae bacterium
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae.
REFERENCE   1  (bases 1 to 1843)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1843)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:19:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,224
            CDSs (total)                      :: 3,198
            Genes (coding)                    :: 3,184
            CDSs (with protein)               :: 3,184
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1843
                     /organism="Aminobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_74988_length_1843_cov_4.298098"
                     /isolate="AMR_MDS_3026"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:3034609"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..495)
                     /locus_tag="P5201_11185"
     CDS             complement(<1..495)
                     /locus_tag="P5201_11185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006583781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HRV99150.1"
                     /translation="MKLRNVLLAALLVFVVAGTAMAADTIKIGVYLPLTGRTAFGGQL
                     ELEGIQMAHKEMSEVLGKKVELVVVDNKTDKVEAANAVKRLIEYDKVVAVIGTYGSSL
                     AMAGGEVAEAAKVPMMGTSCTNPLVTQGKMYVFRACFIDPFQGAGAATYAINSLKMNK
                     AALLI"
     gene            complement(586..>1843)
                     /locus_tag="P5201_11190"
     CDS             complement(586..>1843)
                     /locus_tag="P5201_11190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008710465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="anaerobic ribonucleoside-triphosphate reductase"
                     /protein_id="HRV99151.1"
                     /translation="YGDYASEAKRFLRALFSVYMEGDSWGQPFFFPKPLLHITEDFFR
                     EDGWEEMLSLACEVASEKGNTYFVFDRGDAVKLSECCRLSFELDHRDILEARTPWKLR
                     FCALQNVTLNLPRMAYRSGGDRETLFELVDHYMEIAAKAHLQKKKFISEILDLGKRGP
                     LSVLCMEHDGEPYLRFERVKYLVGILGMNEMVQTITGRQLHESEEAFQLALAVTQYMN
                     LKCQALSERYGLDIVLEQTPAESTAYRFAKLDLKSCPETLDVVKGNPVTGEVYYTNST
                     HFPYNILMDPVRKIVEEGRFHPLISAGAITHIWMGEHKPDARALSSFIRKVFRHSENA
                     QVTFSPEFTVCNGCGVTSRGLRESCPSCGSMDVDGITRITGYFTRTSSWNDGKRAELQ
                     DRVRTSIPDVPSEVGLSRQPSLALEK"
BASE COUNT          417 a          525 c          467 g          434 t
ORIGIN      
        1 gatgagaagc gcggccttgt tcattttgag agagttgatt gcatacgtcg cagcgcccgc
       61 tccctggaag ggatcgatga agcatgctct gaagacatac atcttgccct gggtaaccag
      121 cgggttggtg caggaggtcc ccatcatggg gaccttcgcg gcttcggcga cctcgccgcc
      181 cgccatggcg agagaggatc cgtacgttcc gatgacggca acgaccttat catattcgat
      241 aaggcgcttc acggcgttgg cggcctcgac cttgtcagtc ttgttgtcga cgacgacaag
      301 ttcgactttc tttccaagca cctccgacat ttccttgtgc gccatctgaa tgccttcaag
      361 ttccaactgt ccgccgaaag cggttcttcc ggtcaaggga agatatacgc cgatcttgat
      421 cgtatcggcg gccatagccg ttcccgcaac aacgaaaacc aacagtgcag caagcagtac
      481 atttcgaagc ttcattcggt tccctcctca gatattgata ttcgacttca cgataaatta
      541 tacacaaaaa ctgggacttc cggaagccct ttcttttctt tatttttact tttcaagggc
      601 gagggaaggt tgtcgcgaaa gaccgacttc actgggtaca tcgggaatcg aggtacgaac
      661 cctgtcctga agctccgccc tctttccgtc gttccagctc gaagtcctgg taaaataccc
      721 ggtaattcgc gtaataccgt ccacgtccat acttccgcag gaaggacaac tctctcgaag
      781 accgcgcgac gtcacgccgc agccgttgca aacagtgaat tccgggctga aagtaacttg
      841 cgcgttttcg gagtgacgaa aaactttccg tataaacgaa gaaagagcgc gggcatccgg
      901 tttatgctcg cccatccaaa tgtgcgtaat ggccccggcg gagataagcg gatgaaaacg
      961 tccctcctct acaatcttcc gcacaggatc cataaggata ttgtacggga agtgcgtgct
     1021 gttggtgtag tacacctctc ccgtgacggg gttgcccttc actacgtcga gcgtctccgg
     1081 acacgactta agatccagtt ttgcaaaacg atacgctgtg ctctcggcgg gagtctgctc
     1141 aagcacgata tcgagtccat agcgttccga aagagcctgg catttcaagt tcatgtattg
     1201 cgtgaccgcg agcgcgagct ggaacgcctc ttccgattcg tgaagctgac ggcccgtgat
     1261 cgtctggacc atctcgttca tacctaatat acctacaagg tacttcaccc tctcgaaccg
     1321 aagatacggt tcgccgtcgt gttccataca gagaacagaa agcgggcctc gcttcccaag
     1381 atcaagtatc tcactgatga attttttctt ctgcaggtgc gctttcgcgg caatctccat
     1441 gtaatggtct accagctcga agagcgtctc cctgtctccg ccgctcctgt acgccattct
     1501 ggggaggttg agcgtcacgt tctgcagggc gcagaagcga agcttccacg gtgttctcgc
     1561 ttccaaaata tccctgtgat caagctcgaa gcttaaacgg cagcactcgg agagcttgac
     1621 cgcatctccc cggtcgaaga caaaataggt gtttcccttt tcggacgcga cctcgcaggc
     1681 aagggagagc atctcctccc atccgtcctc gcggaaaaag tcctctgtga tgtgcagcag
     1741 tggctttggg aagaaaaagg gctgccccca gctgtcgccc tccatgtaca ccgaaaagag
     1801 cgctctcagg aaccgcttcg cttcggaagc atagtcgccg tat
//
LOCUS       DAOTIL010000596         1840 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminobacteriaceae bacterium isolate AMR_MDS_3026
            NODE_75161_length_1840_cov_2.586555, whole genome shotgun sequence.
ACCESSION   DAOTIL010000596 DAOTIL010000000
VERSION     DAOTIL010000596.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868050
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminobacteriaceae bacterium (wastewater metagenome)
  ORGANISM  Aminobacteriaceae bacterium
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae.
REFERENCE   1  (bases 1 to 1840)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1840)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:19:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,224
            CDSs (total)                      :: 3,198
            Genes (coding)                    :: 3,184
            CDSs (with protein)               :: 3,184
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1840
                     /organism="Aminobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_75161_length_1840_cov_2.586555"
                     /isolate="AMR_MDS_3026"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:3034609"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..632
                     /locus_tag="P5201_11195"
     CDS             <1..632
                     /locus_tag="P5201_11195"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HRV99152.1"
                     /translation="VSGSVSLFGADLSEASNQELVALRRRCGVVSQDPYGSLPPTLTV
                     LEAVMEPWLLVRGREARQAGEHKARELLDELGIGREEILSSKVRLSLSGGQRQRVAIA
                     RALVLEPELLLCDEPTSMQDASTRGDVLRILQRRQETGMSMIFVTHDLFLARKAASRG
                     LVLYRGEAVEEGLCRDFLASPQHPYTKALVAALPKLSSSATYAGESGGR"
     gene            complement(658..1368)
                     /locus_tag="P5201_11200"
     CDS             complement(658..1368)
                     /locus_tag="P5201_11200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008521862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004523 - RNA-DNA hybrid ribonuclease
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="reverse transcriptase-like protein"
                     /protein_id="HRV99153.1"
                     /translation="MKTPSSTPENHYLSLDADWGEALARIPESSSKKVSGPFRGHFDG
                     ASRGNPGEAGAGALLLDASGTPVWTCARPLGRKTNNEAEYAALILLLEEILARGLAGV
                     SVRGDSQLVVNQVTGRWKVKEPRLQELAERAKELLKATKSSLSWVPREENKDADALSN
                     EALDGPAKPAQSALTGKGADDTAAFPAERLERVTGSIYIAHGSEDYAVDLLHKACTCP
                     SFQHRKRCKHLEAAERLA"
     gene            1537..>1840
                     /locus_tag="P5201_11205"
     CDS             1537..>1840
                     /locus_tag="P5201_11205"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRV99154.1"
                     /translation="MTVTLSPVLSFRRGGASAGSSPHCLKGLFLLLLLFFCFAPILSP
                     LPADAKGPEEIVFLKGLFVKEVRGYGLYVEEESNRFHKDDKPTIYLEVDNFKLGVKD"
BASE COUNT          339 a          569 c          547 g          385 t
ORIGIN      
        1 cggtctcggg gagcgtgtcg ctcttcggcg ccgacctctc cgaagcctcg aaccaggagc
       61 ttgtcgcgct cagacgccga tgcggcgtcg tctcgcagga tccgtacggc tcgctccctc
      121 ctaccctcac cgtgctggag gcggtgatgg agccgtggct tctcgtcaga ggacgcgaag
      181 cgcggcaagc cggggagcat aaggcgcggg aactgctcga cgaactggga atcggccggg
      241 aggaaatttt gtcgtccaag gtcaggctct ccctctccgg cggccagcgt caacgggtcg
      301 ccatcgccag ggcgctcgtg ctcgaaccgg aactcctgct ctgcgacgag cccacgagca
      361 tgcaggacgc ctcgacgaga ggggatgttc tgcggattct ccagcggcgg caggagacgg
      421 ggatgtcgat gatcttcgtc acccacgatc tcttcctcgc gcggaaggcg gcgtcgagag
      481 ggctcgtact ctaccgcgga gaggcggtcg aagaggggct ctgccgcgac ttcctcgctt
      541 cgccccagca cccgtacaca aaggcgctgg tagcggcgct tccgaaactc tcctcttcag
      601 cgacgtacgc cggggagtcc ggcggaagat gacggcgcgg ccggttcacg gcgcgcgcta
      661 cgccaatctc tccgcagcct ccaggtgttt gcaccgcttc ctgtgctgga acgaaggaca
      721 ggtgcacgcc ttgtggagca gatcgacggc gtagtcttcg cttccgtggg cgatatagat
      781 ggagccggta acccgttcca ggcgttcggc cggaaatgcg gcggtatcat ccgcgccctt
      841 ccccgtcaaa gcgctctgag caggctttgc aggaccgtcg agcgcttcgt tggagagcgc
      901 gtcggcgtct ttgttctctt cccgcggaac ccacgagagc gagctcttcg tagccttgag
      961 cagctccttg gcacgctcgg caagctcctg cagacgaggc tccttcacct tccagcgtcc
     1021 ggtgacctga ttcaccacaa gctggctgtc cccccggacg gagacgcccg cgagcccgcg
     1081 cgccagtatc tcctccagca gcaggatgag cgcggcgtac tccgcctcgt tgttggtctt
     1141 ccgcccgagc ggacgggcgc acgtccatac aggcgttccg gacgcgtcga gcagcagcgc
     1201 tcccgccccg gcctcgccgg gattgccccg ggaagcgccg tcgaaatgcc cccggaaggg
     1261 gccggaaact tttttcgaag aggactccgg aatgcgggca agggcttcgc cccagtccgc
     1321 gtcgaggctc agatagtgat tttcaggggt actcgaaggt gttttcatag gatgctcctt
     1381 atcagcaaag gtaactttcg ttaattttcg agaacaaata agccttaaga ttcatttgca
     1441 cgatgtattt tcgataaaca gtatatttcc ctagtccatg aataaaaaaa tctatataat
     1501 gctttgggct tttcattttt atctcaagga ggctgcatga ctgtgacact atcgccagtc
     1561 ctttctttcc ggagaggcgg cgcttccgca ggaagctcgc cgcactgcct taaaggtctt
     1621 tttctccttc ttcttctgtt tttctgcttc gcgccgattc tctcgcctct tcccgccgac
     1681 gccaagggtc ctgaagaaat cgtcttcctt aaaggacttt tcgtcaagga ggtccgcgga
     1741 tacggcctct acgtcgaaga ggagagcaac cgctttcata aagacgacaa accgacgatc
     1801 tacctcgagg tcgataactt caagctggga gtgaaggacg
//
LOCUS       DAOTIL010000597         1839 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminobacteriaceae bacterium isolate AMR_MDS_3026
            NODE_75189_length_1839_cov_3.832960, whole genome shotgun sequence.
ACCESSION   DAOTIL010000597 DAOTIL010000000
VERSION     DAOTIL010000597.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868050
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminobacteriaceae bacterium (wastewater metagenome)
  ORGANISM  Aminobacteriaceae bacterium
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae.
REFERENCE   1  (bases 1 to 1839)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1839)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:19:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,224
            CDSs (total)                      :: 3,198
            Genes (coding)                    :: 3,184
            CDSs (with protein)               :: 3,184
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1839
                     /organism="Aminobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_75189_length_1839_cov_3.832960"
                     /isolate="AMR_MDS_3026"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:3034609"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..360)
                     /locus_tag="P5201_11210"
     CDS             complement(<1..360)
                     /locus_tag="P5201_11210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013049056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HRV99155.1"
                     /translation="MKKFAAVLAACAVLSISGAAFANAIVMNGSTTVLPIAQAAAEKY
                     MAANSDVKISVSGGGSGNGIKAIIDGTTDIADSSRFIKTDEVKAAVEKGTYPVPFAVA
                     MDALLPIVHPENPVSDLS"
     gene            598..1011
                     /locus_tag="P5201_11215"
     CDS             598..1011
                     /locus_tag="P5201_11215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008519796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicB family
                     antitoxin"
                     /protein_id="HRV99156.1"
                     /translation="MKSERTTAIYPAIFSHAEDGITITFPDLPGCISCAQTDEEALHM
                     ARDALGAWIVATEDLGEPVPLPSPTSKLRPKKNEAVCLVDVWLPLFREEKRSGSVKKN
                     VTVPLWLNVLAEKAGLNFSQILQAGLKSTLGIQDR"
     gene            complement(1267..1488)
                     /locus_tag="P5201_11220"
     CDS             complement(1267..1488)
                     /locus_tag="P5201_11220"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRV99157.1"
                     /translation="MPMTVEREKLAQNILALTDEEVSAVAAFVRNLREDEPPLSDDEL
                     AQMAESDEDIAAGRLTPWKEARRRLEALP"
BASE COUNT          433 a          516 c          504 g          386 t
ORIGIN      
        1 gctcaggtcg ctcacggggt tctccgggtg cacgatggga agcagcgcgt ccattgccac
       61 cgcgaagggc accgggtacg ttcccttctc caccgcagcc ttcacctcgt ccgtcttgat
      121 gaagcgggag gagtcggcga tgtccgtggt gccgtcgatg atcgccttga tgccgttgcc
      181 gctgccgccg ccggaaacgc tgatcttgac gtcgctgttg gccgccatgt acttctccgc
      241 cgccgcctgg gcgatgggaa gcaccgtggt ggaaccgttc atcacaatgg cgttcgcaaa
      301 cgccgcgccg gaaatggaga ggaccgcaca tgccgccaga actgccgcga atttcttcat
      361 accgaatagc ctccttggat tttcgtctta gtctgcgtcg gggaccatcc ccgatcgaca
      421 cgcacagtat aggaggcgag cgtaacggat gttacaaaag tatgtaacag atgagtaacg
      481 atgacgttac gttcgtaacg gggggggaag atgcgcataa aaaaggagta cgcatatatc
      541 atgctggacg ctacctactc aaacgtctag aatatacaca aaaggggagg cgaaataatg
      601 aaaagcgaac gcacaaccgc aatctatccg gcgatcttct cccatgcgga agacggtatc
      661 acaataacct tccccgacct cccgggatgc atttcctgcg ctcaaacaga cgaggaggcg
      721 ctgcatatgg cacgagacgc cctcggagca tggatcgtcg ccaccgagga cttgggagaa
      781 ccagtcccgc tgccctctcc tacttcgaaa ctacgcccga aaaagaacga agccgtctgc
      841 ctggtcgacg tgtggctgcc gctcttccgc gaagaaaagc ggtccggctc cgtaaagaaa
      901 aatgtcacgg tcccgctttg gctgaacgtg ctggctgaaa aagccgggct gaacttctcc
      961 caaattctcc aggcagggct taaatccact ctcggaattc aagaccgttg aggagctgtg
     1021 gaaaatgacg gaccacaaga ttgaagacat gtggatcaaa gaagcctccc gccgggaacg
     1081 tgagatggat acgggcatgg cacgttgcat acctgcggat gaggcgctgg aaagagctta
     1141 tcaacggcgc aggagcagag agtagctgtt tttttaaaaa acacccgggg aaatatggag
     1201 gctttgcttt tatttttcat caccatgctg attcaagaag atcacgaaaa gagtgtgtta
     1261 ccaacgtcac ggcaacgcct caagacgtcg tcttgcctct ttccaagggg tcagacggcc
     1321 cgccgcgatg tcttcgtcgg actcggccat ctgtgccagt tcgtcgtcgc tcaatggcgg
     1381 ctcgtcctca cgcagattgc gcacaaacgc agccaccgca gaaacttcct cgtccgtaag
     1441 tgccagaatg ttttgcgcca gcttttcccg ttccacggtc atcggcatag tcgtcacccc
     1501 cctttcatcg tcattatagc gtgcttcata gcgcaccgca gagagagcgt ccattaaagc
     1561 atgatgcctc tcaggaaaca ggctcggcct tcttcccgga ctcctgttca agaaagaccg
     1621 gtggctgtat gctattatga gcgtcgggag ttgtttaaaa aaaggagggg cttcaccgat
     1681 ggaagatatc cggtggatac agcgattcga cagctttctt cgcgcgcttt cccaacttga
     1741 agaagcatac gcgcttgccg cgtcgcgtcg gctctcccgc cttgaggagc aggggcttat
     1801 tcaagcgttc gaattcaccc atgaactggc atggaaaac
//
LOCUS       DAOTIL010000598         1837 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminobacteriaceae bacterium isolate AMR_MDS_3026
            NODE_75304_length_1837_cov_3.482043, whole genome shotgun sequence.
ACCESSION   DAOTIL010000598 DAOTIL010000000
VERSION     DAOTIL010000598.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868050
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminobacteriaceae bacterium (wastewater metagenome)
  ORGANISM  Aminobacteriaceae bacterium
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae.
REFERENCE   1  (bases 1 to 1837)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1837)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:19:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,224
            CDSs (total)                      :: 3,198
            Genes (coding)                    :: 3,184
            CDSs (with protein)               :: 3,184
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1837
                     /organism="Aminobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_75304_length_1837_cov_3.482043"
                     /isolate="AMR_MDS_3026"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:3034609"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..783)
                     /locus_tag="P5201_11225"
     CDS             complement(<1..783)
                     /locus_tag="P5201_11225"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRV99158.1"
                     /translation="MPSLCSLKKTLFRSSAPLIAGVVSLLFILGCVSRADAGQQKKNI
                     LVLHSFHQGYEWTDSVQQGILDEMAPRRKDVSLSVEYLDSIRSPEASQLEAARELFRR
                     RYAHGKTLFDVILCSDDEALGFLLEYGGELFGDVPVVFCGVNNFSQERLGGRKNITGV
                     NETISVRETIEIALKLRPSAKTVAVVAGFRPAEIRNLEMAKAAEPFFKGRVAFQYLHG
                     FEPEEIANRLKELSSEDIVLYLSYLLSPSGKRLSVEENRTFVV"
     gene            1061..>1837
                     /locus_tag="P5201_11230"
     CDS             1061..>1837
                     /locus_tag="P5201_11230"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_process: GO:0009966 - regulation of signal transduction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HRV99159.1"
                     /translation="MRRDYLRMLDTLKEGVYFVDLDRKITFWNKGAERITGFRREEVV
                     GSSCMDNILVHVDCKGTQLCKEGCPLLYTMKDGSSREAEVFLHHRRGHRVAISVRTTP
                     LEDDGGNIIGGIELFSELNPEESLRQRMEELERCAMIDPLTGVPNRSYLNPELERLFR
                     LWEANRIPFGLLFFDIDHFKRFNDTYGHDVGDQVLRTVAQSLSSSVRNYDSVGRWGGE
                     EFVGFFPNANPEVLLPIAEKLRQVVRNSWVEIEGEQLSVTI"
BASE COUNT          447 a          512 c          515 g          363 t
ORIGIN      
        1 gacgacgaac gttctgttct cctcgaccga gagccttttc cctgaagggg agaggaggta
       61 ggagaggtag aggacgatat cttcggatga aagctccttc agccgatttg cgatctcctc
      121 cggttcgaaa ccgtgcaaat actggaacgc cactcttccc ttgaagaaag gttccgcggc
      181 ctttgccatc tccaggttgc gaatttcggc ggggcggaaa ccggccacca cggcgaccgt
      241 cttcgcggaa ggacgaagct tcagggcgat ctctatcgtc tccctgacgg agatcgtttc
      301 gttgacgccc gtaatgttct tccttccgcc gaggcgctcc tgtgaaaagt tattgacgcc
      361 gcagaagacg acggggacgt cgccgaagag ctctccgccg tactccagaa gaaagccaag
      421 cgcttcgtcg tcgctgcaga ggatgacgtc gaaaagcgtt ttcccgtgtg cgtaccggcg
      481 gcggaagagt tcgcgcgccg cctccagttg gctcgcctcc ggagaacgta tggagtccag
      541 atactccacg gagagcgata cgtccttccg ccggggcgcc atctcgtcga ggatgccttg
      601 ctggacggaa tcggtccact cgtacccctg atggaacgag tgcagcacga ggatattttt
      661 cttttgttgc ccggcgtcag cccgcgaaac gcatccaaga atgaagagca gcgacacaac
      721 gcccgcgatc agcggtgctg aagaacgaaa cagagttttt ttcaaggaac aaagagatgg
      781 caagaacacc tcttcaccag ctttcagtta taataaaagc gtcaataagc gctatggcgt
      841 gcggcttcct aaaaagcgaa ttgtcatgta ctattcatac caagcatttg taaatctcac
      901 tatatcgcac aaaatctttg atgtaaagaa aacatcggga aagaacacac gaagacgctg
      961 cctggagcaa acggtgaatc ggcgcaaaca aaaccagagt atccttctgt actttttaga
     1021 aataaaattg aaaacagcct cctacgaaag gatgaaaaga atgcggcggg actatcttcg
     1081 gatgctggat acgctcaagg aaggagtcta cttcgtcgac ctcgacagaa agatcacctt
     1141 ctggaacaag ggcgcggagc gaatcaccgg gtttcgcaga gaggaagtcg tcgggagctc
     1201 gtgcatggac aacatcctcg tgcacgtcga ctgcaaggga acccagcttt gcaaagaggg
     1261 gtgtccgctc ctctacacca tgaaggacgg ctcctccaga gaggccgagg tttttctgca
     1321 tcacaggaga ggacacaggg tcgccatctc cgtgcgcaca accccgctcg aggacgacgg
     1381 gggaaacatc atcggcggca tcgagctctt cagcgagctc aaccccgagg agagtttgcg
     1441 tcagcggatg gaggagcttg aacggtgcgc gatgatcgac ccgctcaccg gcgtgccgaa
     1501 caggagttac ctgaatccgg agctggaacg cctcttccgc ttatgggagg ctaaccgcat
     1561 cccgttcgga ctgctcttct tcgatatcga ccacttcaag cggttcaacg acacctacgg
     1621 acacgacgtc ggcgaccagg tgctgcgaac ggtcgcgcaa tcgctctcat cctccgtcag
     1681 aaactacgac agcgtcggac gctggggggg cgaggagttc gtcggcttct tcccgaacgc
     1741 caacccggag gtgctcctcc cgatcgctga aaagcttcgc caggtcgttc gcaactcctg
     1801 ggtcgaaata gaaggagaac agctctcggt gaccatc
//
LOCUS       DAOTIL010000599         1836 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminobacteriaceae bacterium isolate AMR_MDS_3026
            NODE_75361_length_1836_cov_3.145424, whole genome shotgun sequence.
ACCESSION   DAOTIL010000599 DAOTIL010000000
VERSION     DAOTIL010000599.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868050
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminobacteriaceae bacterium (wastewater metagenome)
  ORGANISM  Aminobacteriaceae bacterium
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae.
REFERENCE   1  (bases 1 to 1836)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1836)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:19:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,224
            CDSs (total)                      :: 3,198
            Genes (coding)                    :: 3,184
            CDSs (with protein)               :: 3,184
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1836
                     /organism="Aminobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_75361_length_1836_cov_3.145424"
                     /isolate="AMR_MDS_3026"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:3034609"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(78..605)
                     /gene="ruvC"
                     /locus_tag="P5201_11235"
     CDS             complement(78..605)
                     /gene="ruvC"
                     /locus_tag="P5201_11235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008522791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008821 - crossover junction
                     endodeoxyribonuclease activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="crossover junction endodeoxyribonuclease RuvC"
                     /protein_id="HRV99160.1"
                     /translation="MLKCIGIDPGLGRMGVATVLQDGRSFRLISGGCIETSPGEEISS
                     RLDTIFRKLGEQIECCSPDFMSVEKLFFGRNITTAEYVWQARGIALLLAARYRLPLFE
                     PKPSQIKLTVCGYGTAPKEQVQKMVQRLLGLKEVPKPDDVADAIGAAITGFSLFAEKR
                     RISSAGGYHASLSRR"
     gene            complement(609..1361)
                     /locus_tag="P5201_11240"
     CDS             complement(609..1361)
                     /locus_tag="P5201_11240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012869672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HRV99161.1"
                     /translation="MSGHSKWANIKHRKGAQDAKRGNLFQKLVKAIIIAAKEGGGDPS
                     MNMRLKAAIERAKAASVPNDNITRGIKRGTGEIDGGQFDEILYEAYGPNGVAILIEVL
                     TDNRNRAASEMRTLLTRNGGSLGEAGSVAWMFERKGVIEVSGKDLDEEALMTVALEAG
                     AEDLQNQDDGFIVYSEPSVYGLVRDALEKAGYVVSRAETEMVPKNTVMIANKEKAQKI
                     LRLMDLIEEHDDVQNAYGNFDIPDEILESLDA"
     gene            complement(1395..>1836)
                     /gene="nadE"
                     /locus_tag="P5201_11245"
     CDS             complement(1395..>1836)
                     /gene="nadE"
                     /locus_tag="P5201_11245"
                     /EC_number="6.3.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006301050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008795 - NAD+ synthase activity [Evidence
                     IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="NAD(+) synthase"
                     /protein_id="HRV99162.1"
                     /translation="HAFGEPLSPMAAANIKPRLRMNALYALAQTRRFLVCGTGNKAEI
                     LMGYFTKYGDGGCDLLPLGDLLKSEVRALAGFLKVPDEIIQKAPSAGLWDGQTDEEEM
                     GMTYEEIDAYIATGKGDEKVRTRIEQMKNVSAHKRAMPPVCIPF"
BASE COUNT          414 a          543 c          419 g          460 t
ORIGIN      
        1 cacgccctgt acaaaggagt tccaaaccca aaccgttgac ttcgagcacg actatattgt
       61 caattatcga gtgaacatta cctgcgagaa agcgaagcat gatagcctcc tgcactggaa
      121 atacgccgct tttccgcaaa aagcgaaaaa ccggtaattg cagctcctat agcatcagcg
      181 acgtcgtccg gcttgggaac ttctttaagc cccaagaggc gctgtaccat tttctgcacc
      241 tgttccttcg gcgccgttcc atacccgcat accgttaact tgatctgaga gggttttggt
      301 tcaaaaaggg gtagacggta acgggctgcg aggaggagcg ctatgccgcg tgcctgccac
      361 acgtactccg cagtcgtgat gttccttccg aaaaaaagct tttcaaccga cataaaatca
      421 ggggagcagc attcaatctg ctccccgagt tttctgaaga tcgtgtccag tcgggaagag
      481 atttcctctc cgggcgatgt ttcaatacac ccgcccgaga taagacggaa agagcgtcca
      541 tcctgaagga ccgttgccac tcccatccgg cccagccccg ggtcgatccc gatacacttc
      601 agcaacgctt acgcatccag gctttccaga atttcatccg gaatgtcgaa gttgccgtag
      661 gcattctgga cgtcgtcgtg ttcttcgata agatccataa gacgcaaaat cttctgcgct
      721 ttttccttat ttgctatcat aacagtattc ttcgggacca tctcggtctc ggcccggctg
      781 acaacatagc ctgctttctc caaggcgtcc cgcaccagac cgtaaacact cggctcactg
      841 tatacaataa agccgtcgtc ctgattctgc aggtcttccg ctccagcctc cagggcaact
      901 gtcatcaagg cctcttcgtc aagatctttc cctgaaactt caatgacgcc tttccgctcg
      961 aacatccacg caacgcttcc tgcttcacca agggaaccgc cgtttcttgt cagaagggtg
     1021 cgcatctcgg atgcagccct gttcctgtta tcggtaagaa cctcgatcag aatggcgacg
     1081 ccgttcgggc cgtatgcctc gtaaagaatc tcgtcgaact gtccgccatc gatttcaccg
     1141 gtaccgcgct taattcctct cgttatgttg tcgttgggta cgctcgcagc cttcgcccgt
     1201 tcaatggcgg ccttcaggcg catattcatc gagggatcgc ccccgccctc tttcgcggcg
     1261 ataatgatgg cctttacaag cttctggaaa aggtttccgc gctttgcatc ctgcgctcct
     1321 tttctatgtt tgatattcgc ccatttagaa tgtcccgaca aagaaaatca ctcctctgga
     1381 aaatatatac actctcagaa tggaatacac acgggcggca tcgccctttt atgcgccgac
     1441 acgtttttca tttgttcaat ccgtgtcctg actttttcat ctccctttcc agtggcaatg
     1501 taggcgtcta tctcttcgta cgtcatgccc atctcttcct cgtccgtctg tccgtcccaa
     1561 agacccgccg aaggagcctt ttggataatc tcatccggaa ctttcaggaa cccggcgagc
     1621 gcacgaactt cactcttcag aagatcaccg agaggaagaa gatcgcagcc gccatctccg
     1681 tacttggtaa aataccccat gagaatctcg gccttgttgc ccgtgccgca tacgaggaaa
     1741 cggcgggtct gcgccaaggc atagagggca ttcatacgaa gcctcggttt tatgttcgct
     1801 gcagccatcg gagaaagagg ttctccaaag gcatgt
//
LOCUS       DAOTIL010000600         1834 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminobacteriaceae bacterium isolate AMR_MDS_3026
            NODE_75491_length_1834_cov_3.225408, whole genome shotgun sequence.
ACCESSION   DAOTIL010000600 DAOTIL010000000
VERSION     DAOTIL010000600.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868050
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminobacteriaceae bacterium (wastewater metagenome)
  ORGANISM  Aminobacteriaceae bacterium
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae.
REFERENCE   1  (bases 1 to 1834)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1834)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:19:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,224
            CDSs (total)                      :: 3,198
            Genes (coding)                    :: 3,184
            CDSs (with protein)               :: 3,184
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1834
                     /organism="Aminobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_75491_length_1834_cov_3.225408"
                     /isolate="AMR_MDS_3026"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:3034609"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            271..356
                     /locus_tag="P5201_11250"
     tRNA            271..356
                     /locus_tag="P5201_11250"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:305..307,aa:Ser,seq:tga)
     gene            complement(453..1217)
                     /locus_tag="P5201_11255"
     CDS             complement(453..1217)
                     /locus_tag="P5201_11255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014163813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarylacetoacetate hydrolase family protein"
                     /protein_id="HRV99163.1"
                     /translation="MEKTFIRCIIRGEERQALVADGAVYALRAPMGDGAEPGERLGTL
                     DEVERFLPPTSPTKVVAVGLNYRDHAEEVGLPIPEEPLIFIKPSTSVVGHGDPVVYPD
                     VMTKRVDYEAELGIVIGKRCRSVSPEDALGYVLGYTCANDVTARDLQSKDGQWTRSKS
                     FDTFCPLGPFVVSGVDPSDLEITMLVNGKVVQHSRTSNFIFPVPVLVSHISHVMTLEP
                     GDVIITGTPAGIGPVKKGDLMRVEIERLGALENRVE"
     gene            1481..>1834
                     /locus_tag="P5201_11260"
     CDS             1481..>1834
                     /locus_tag="P5201_11260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013047693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PhoU domain-containing protein"
                     /protein_id="HRV99164.1"
                     /translation="MFGKKNGKVESLLYSGDREKILAMVREMGSMASEAFRLAVQSLE
                     ERDDALAERVIEEDDAIDDLEATIDHECLCSIAMRQPVREDLRFVFAVLKIITDLERI
                     GDQGVNIAKKAKVLNR"
BASE COUNT          405 a          548 c          505 g          376 t
ORIGIN      
        1 cgccggaaag gtagttcatt acccgaaaca ccggacggtc ggcctttggc gacgccccga
       61 cgaccgcaac tcgacagggt gtacagacgt accgtttcaa aatttcttct ttcttcactc
      121 caatcacctc tcccgaagaa tcgttgccgc atgcactctt cttgagtgca acgctattat
      181 accccacgcg caagaagcga aaaacagccg tctttcctcc cgcagcacgt tggcaagagt
      241 acaaaagatg tgtataatgc tcttcatgct ggagggctgt ccgagcggtc gatggtggtt
      301 gacttgaaat caactgggcg taagccccag gggttcgaat cctctgccct ccgccattca
      361 catgcatgat cggacgtttt tacgtccgca gacgaaaagg gggaacgcga gcggaacgcg
      421 gtcccccttt ttgcgtatgc ttccgaatca ggctactcga cccggttctc cagcgcaccc
      481 agacgttcga tctcgacgcg catcagatcc cccttcttca ccggcccgat tccagccggg
      541 gtgcctgtga tgatcacatc gccgggttcc agcgtcatca cgtgcgagat gtggctgacg
      601 agcaccggca cggggaagat gaagttgctc gtgcgggagt gctggacgac cttcccgttc
      661 accagcattg taatttccag gtccgaagga tcgaccccgg acacgacgaa cggcccgagc
      721 ggacagaacg tatcgaagga cttcgaccgc gtccactgcc cgtccttcga ctgaagatcc
      781 ctcgcggtca cgtcgttcgc gcaggtgtac cccaaaacgt agccgagcgc atcctccggc
      841 gacacgctcc tgcacctctt cccgatgacg atgccgagtt cggcctcgta atccacccgt
      901 ttggtcataa cgtccggata cacgaccgga tcgccgtgcc ccacgacgct cgtcgagggt
      961 ttgatgaaga tgagcggctc ctcgggtatc ggaagcccca cctcctcggc atgatccctg
     1021 tagttcaacc cgaccgcgac gaccttcgtg ggcgaagtcg gagggagaaa acgctcgacc
     1081 tcgtcaaggg ttccgaggcg ctctcccggc tctgctccgt ctcccatggg agcgcgcaga
     1141 gcatacactg cgccgtcggc aaccagcgcc tgccgctcct ctccgcggat gatgcatcgt
     1201 ataaaagtct tttccatagt ccatctctcc tccagggcaa aatctaccat cttgaaatgt
     1261 ataatagcag attcgcagca cagaaaacga tgtaccgcga aaaaagtgtt gaactggagc
     1321 acacgggcgc tccctcacaa ttgactttca ggatggcata tcgtatcctg taacattatc
     1381 gttacaaaaa cattgccccg gttgacgctt cccgtacgag tccttctccg acaagggaaa
     1441 tacaacgggg ccgttgatgt atttcgagga ggggtggagt gtgttcggaa agaagaacgg
     1501 gaaggtcgaa tcccttctct attcaggaga cagagagaag attctcgcca tggtccgcga
     1561 gatgggcagt atggcgagcg aggcgttcag actcgctgtc cagtcgctcg aagagcgcga
     1621 cgacgcgctt gcggagaggg ttatcgaaga agacgacgca atagacgacc tggaggcgac
     1681 gatcgaccat gagtgtttgt gctcgatcgc catgcgtcag cctgtccgtg aggatctccg
     1741 cttcgtcttc gcggttttga agatcatcac cgacctggag cgcatcggcg accagggggt
     1801 caacatcgcg aagaaggcga aggtgctcaa ccgg
//
LOCUS       DAOTIL010000601         1832 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminobacteriaceae bacterium isolate AMR_MDS_3026
            NODE_75630_length_1832_cov_2.621835, whole genome shotgun sequence.
ACCESSION   DAOTIL010000601 DAOTIL010000000
VERSION     DAOTIL010000601.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868050
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminobacteriaceae bacterium (wastewater metagenome)
  ORGANISM  Aminobacteriaceae bacterium
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae.
REFERENCE   1  (bases 1 to 1832)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1832)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:19:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,224
            CDSs (total)                      :: 3,198
            Genes (coding)                    :: 3,184
            CDSs (with protein)               :: 3,184
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1832
                     /organism="Aminobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_75630_length_1832_cov_2.621835"
                     /isolate="AMR_MDS_3026"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:3034609"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..758
                     /locus_tag="P5201_11265"
     CDS             <1..758
                     /locus_tag="P5201_11265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015556749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HRV99165.1"
                     /translation="LIIAGGSAYPRLIDYERIARIAHSAGGLLMVDMAHVAGLVAAKV
                     IPSPVPHADFVTSSTTKTLCGPRGGMVLCKAEHAKALDKGVFPGAISSIHLNIMAAKT
                     WLFKHAASEEFKQVMTQIVANSRALAAELTGYGFRVISGGTDNHIVLLDLRPKKITGK
                     LFQDALEYVGITINKNMIPSDPEKPSVTSGVRIGTTSVSQRGLKEPEIKRIAAIMNEV
                     AGAPEDKNVLDACKAEAMRLISAFPLYPKGPLE"
     gene            complement(884..1291)
                     /locus_tag="P5201_11270"
     CDS             complement(884..1291)
                     /locus_tag="P5201_11270"
                     /inference="COORDINATES: protein motif:HMM:NF037716.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclophilin-like fold protein"
                     /protein_id="HRV99166.1"
                     /translation="MSTHNGDGATMLLGIGEKTFEVEMYDSGAARELMACLPQTLSMS
                     RWGDEFYGTLHPKIGHAGDTLRDVFEIGEVAMWPPGNAFCIFFGPTPASVGAEPRAAS
                     PCVPLGRIKGDATLFRNFGSSLKNATLTAGNQV"
     gene            complement(1350..>1832)
                     /gene="ybaK"
                     /locus_tag="P5201_11275"
     CDS             complement(1350..>1832)
                     /gene="ybaK"
                     /locus_tag="P5201_11275"
                     /inference="COORDINATES: protein motif:HMM:TIGR00011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0002161 - aminoacyl-tRNA editing activity
                     [Evidence IEA];
                     GO_process: GO:0043039 - tRNA aminoacylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Cys-tRNA(Pro) deacylase"
                     /protein_id="HRV99167.1"
                     /translation="DMTPGINAAKKARIPYTLHEYEHDPSSESYGLEAAEKLGVPPER
                     VFKTLVVALDGKELVVGIIPVDALLSMKLVAKAAGAKKAVMAQPADVERATGYVLGGV
                     SPLGQKKRLRTFIDSSAEAFSTILVSAGRRGLDIELQPNDLKKLTGASFVPLRQQEGE
                     "
BASE COUNT          315 a          619 c          577 g          321 t
ORIGIN      
        1 ggctgatcat cgcgggcggc agcgcctacc cgcgcctgat cgactacgag cggatcgcac
       61 gtatagcgca ctccgccggc ggcctgctga tggtggacat ggcgcacgtc gccggcctcg
      121 tcgcggcgaa ggtcattccc agccccgtgc cgcacgccga cttcgtcacc tcgtccacga
      181 cgaagacgct ctgcgggccg cgcggcggca tggtcctctg caaggcggag cacgcgaagg
      241 cgctggacaa gggggtcttc cccggcgcga tcagctccat ccatctgaac atcatggcgg
      301 cgaagacgtg gctcttcaag cacgcagcct ccgaagagtt caagcaggtc atgacgcaga
      361 tagtggcgaa ttccagggcg ctggcggcgg aactgaccgg ctacggcttc cgcgtgatca
      421 gcggggggac ggacaaccac atcgtcctgc tcgatctgcg cccgaagaag atcaccggca
      481 agctcttcca ggacgccctc gaatacgtgg gcatcacgat caacaagaac atgatcccct
      541 ccgacccgga gaagccctcc gtcaccagcg gcgtacgcat cgggacgacc tccgtctcgc
      601 agcgcgggct gaaggagccg gagatcaagc ggatcgccgc gatcatgaac gaggtcgccg
      661 gggcccccga ggacaaaaac gtcctcgacg cctgcaaggc cgaggcgatg cggctgatat
      721 cggctttccc gctctacccg aaggggccgc tggagtaagg gaaacagaac ccgtatatcc
      781 tcccccggcg gattgcgtta gcttcgaacg aggggtctgc gacagcgaag cgcctgagtg
      841 atccctgcag aaacgtgccg gatctctcgg gcgcttttta tttttagacc tgatttccgg
      901 cggtgagcgt cgcgttcttc agcgacgaac cgaagttccg gaagagcgtc gcgtccccct
      961 tgatcctccc gagcggaacg cacggcgagg ctgcgcgggg ctcggccccg acgctggcgg
     1021 gggtggggcc gaagaagatg cagaaggcgt tgcccggcgg ccacatcgcg acttcgccga
     1081 tctcgaagac gtcgcggagc gtgtcccccg cgtgcccgat cttcggatgc agcgtgccgt
     1141 agaactcgtc gccccagcgc gacatggaga gagtctgcgg cagacaggcc atcagctccc
     1201 gcgccgcgcc ggagtcgtac atctccacct cgaaggtctt ctcgccgatg ccgaggagca
     1261 tcgtcgctcc gtctccgttg tgcgtgctca tatgcatctc ctctctttct tcgttgattc
     1321 cgaaaagagc gccccgcagg cggctctttc tattcgccct cctgctgccg caaggggacg
     1381 aaggacgcgc cggtcagctt cttcaggtcg ttcggttgca gctcgatgtc cagccctctc
     1441 cgccccgcgc tgacgagaat cgtggagaag gcttcggcgg acgagtcgat gaaggttctg
     1501 aggcgcttct tctgcccgag cgggctcacc ccgccgagga cgtaccccgt ggcgcgctcg
     1561 acgtcggcgg gctgcgccat gacggccttc ttcgcccctg cagccttcgc gaccagcttc
     1621 atgctcagca gggcgtcgac ggggatgatc cccacaacca actccttgcc gtcgagcgcg
     1681 accaccagcg tcttgaagac gcgctcgggc ggcacgccga gcttctccgc cgcctccaac
     1741 ccgtacgact cgctcgacgg gtcgtgttcg tattcgtgca gcgtgtaagg gatgcgcgcc
     1801 ttcttcgccg cgttgattcc cggtgtcatg tc
//
LOCUS       DAOTIL010000602         1830 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminobacteriaceae bacterium isolate AMR_MDS_3026
            NODE_75757_length_1830_cov_2.669859, whole genome shotgun sequence.
ACCESSION   DAOTIL010000602 DAOTIL010000000
VERSION     DAOTIL010000602.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868050
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminobacteriaceae bacterium (wastewater metagenome)
  ORGANISM  Aminobacteriaceae bacterium
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae.
REFERENCE   1  (bases 1 to 1830)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1830)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:19:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,224
            CDSs (total)                      :: 3,198
            Genes (coding)                    :: 3,184
            CDSs (with protein)               :: 3,184
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1830
                     /organism="Aminobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_75757_length_1830_cov_2.669859"
                     /isolate="AMR_MDS_3026"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:3034609"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..246
                     /locus_tag="P5201_11280"
     CDS             <1..246
                     /locus_tag="P5201_11280"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRV99168.1"
                     /translation="GMDGFEFLKELRRRKHDIPVLVLTAKVLTREDTKFLNENGIRFL
                     ALKGDVDPGDLVRKIRKMTSQGAPLADKESLGEGTEK"
     gene            243..1289
                     /locus_tag="P5201_11285"
     CDS             243..1289
                     /locus_tag="P5201_11285"
                     /inference="COORDINATES: protein motif:HMM:NF014069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HRV99169.1"
                     /translation="MNRILAIDDKADNLVVLRAMMKMLLPECVLLQALSGEEGLRIAK
                     EQRPDTILLDIFMPGMDGYETTIRLKADPETASIPVILLTAAENTAANRTAGLKVGAD
                     AFLSKPIEEEELVAQVRAMLRVKESETALKMKMDVLEDLVEQRTKELKESANRLALTL
                     DQTISVLANTVETKDPYTAGHQVRCASLALALGNELRLDPQQLRGIHMAARIHDIGKI
                     KIPSEILSKPGKITPLEFSLIKEHPGVGEAIVAPIVFPWPVGRMIAQHHERIDGTGYP
                     KGLFGDDILMEAKVIAVADVVEAISSYRPYRPALGIDAALQEIENGIGTKYDPDAVRA
                     CTALFRENRFEFDE"
     gene            complement(1350..>1830)
                     /locus_tag="P5201_11290"
     CDS             complement(1350..>1830)
                     /locus_tag="P5201_11290"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HRV99170.1"
                     /translation="EEVDLCRLLSAVVEENRRHPLAGRIGVEEEYECAAPAVVRSEDI
                     SRALNNIVENAVKYVHEKFGDEEGGRILVSLRPEQLFWSIRVEDNGPGISSETAGVLF
                     QRFRRGDSHRARGKWGKGGYGLGLAIARKIVLDAGGDILLHPSDEGAVFEVLLPRKD"
BASE COUNT          395 a          607 c          518 g          310 t
ORIGIN      
        1 ggtatggacg gcttcgaatt tctgaaagaa ctgcgcaggc ggaagcacga cattcctgtc
       61 cttgtcctca ccgccaaggt actcacacgg gaggacacaa aatttctcaa tgagaacgga
      121 attcgctttc ttgccctcaa gggggacgtc gacccgggag accttgtgcg gaaaatccgg
      181 aagatgactt cgcagggcgc gccccttgcg gacaaagaga gcctcgggga gggaaccgaa
      241 aagtgaacag gatccttgcg atcgacgata aggccgataa tctcgtggtg cttcgggcaa
      301 tgatgaagat gctccttccc gaatgcgttc tgctgcaggc gctctcgggt gaagagggac
      361 tccgcatcgc caaggagcag cgccccgata cgattctgct cgacattttc atgccgggga
      421 tggacggcta tgaaacgacg atacgcctca aggccgaccc tgaaacggca tccattccgg
      481 tgatcctcct gaccgccgcc gaaaacacgg cggcgaaccg caccgcggga ctgaaggtcg
      541 gcgccgacgc gttcctctcc aagcccatcg aggaggagga actggttgcg caggtgcgcg
      601 cgatgctccg cgtcaaggag tccgaaacgg cgctgaagat gaagatggac gtactcgaag
      661 atctggtcga acagagaacc aaagagctga aggagagcgc gaaccgcctt gccctgaccc
      721 tcgaccagac catctccgtg ctggcgaaca ccgtggagac aaaagacccg tacacggcgg
      781 gacatcaggt ccgctgcgcc tccctcgccc tcgccctggg gaacgagctg aggctcgacc
      841 cccagcagct tcgcggcatc cacatggccg caagaatcca tgatataggg aagatcaaga
      901 tcccctccga gatcctcagc aaaccgggga aaatcacccc gctcgagttc tcgctcataa
      961 aggaacatcc gggcgtcggc gaggccatcg tcgcccctat cgtctttccg tggcccgtcg
     1021 gccggatgat cgcacagcat cacgaacgaa tcgacggcac ggggtatccc aaagggctct
     1081 tcggcgacga catcctgatg gaggcgaagg tcatcgccgt cgccgacgtc gtggaggcca
     1141 tctcctccta ccgcccctac cgcccggcgc tcgggataga cgccgcgctc caggagattg
     1201 aaaacggcat cggaacgaaa tacgatcccg acgccgtccg cgcatgcact gccctcttca
     1261 gggaaaaccg attcgaattc gacgaatgaa aaagggcgga agagcccgtt tcggagctct
     1321 tccgccctgc gttcctcttt cgccgcggat cagtccttcc ggggcagcag cacctcgaag
     1381 acggcgcctt cgtcggacgg gtgaagtagt atatcccctc ccgcgtcgag cacgatcttc
     1441 ctcgcaatcg ccaggccgag cccgtagccg cccttccccc acttcccgcg cgcccggtgg
     1501 ctgtcgcccc tgcggaaacg ctggaaaagc acgcccgcag tctcggacga aatccccggc
     1561 ccgttatcct caacccgaat gctccagaaa agctgctccg gccgaagcga caccaaaatt
     1621 ctgccgccct cttcgtcgcc gaacttctca tggacatact tcaccgcgtt ctcgacgata
     1681 ttgttcagcg cgcgggagat atcctcgctc cgcaccacgg cgggggccgc gcattcatac
     1741 tcttcctcga ccccgatgcg ccccgcgaga ggatgtctcc ggttctcctc gaccacggcg
     1801 gacagcagcc tgcacaggtc gacctcctcc
//
LOCUS       DAOTIL010000603         1827 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminobacteriaceae bacterium isolate AMR_MDS_3026
            NODE_75905_length_1827_cov_4.020880, whole genome shotgun sequence.
ACCESSION   DAOTIL010000603 DAOTIL010000000
VERSION     DAOTIL010000603.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868050
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminobacteriaceae bacterium (wastewater metagenome)
  ORGANISM  Aminobacteriaceae bacterium
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae.
REFERENCE   1  (bases 1 to 1827)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1827)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:19:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,224
            CDSs (total)                      :: 3,198
            Genes (coding)                    :: 3,184
            CDSs (with protein)               :: 3,184
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1827
                     /organism="Aminobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_75905_length_1827_cov_4.020880"
                     /isolate="AMR_MDS_3026"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:3034609"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..412
                     /locus_tag="P5201_11295"
     CDS             <1..412
                     /locus_tag="P5201_11295"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRV99171.1"
                     /translation="TPADGKAFALGEVALLRGTVRNEGVLPVGNISLFFYDGNPDGGG
                     KPIANRIVSGIVPGGEYSVVLTHSFDTPGPRSVFMRVVPKVGDPPEDNTVSVAVTVQG
                     GDSGGGGCAVGTAGAWSALLLIGLAPLAFGRKER"
     gene            416..1609
                     /locus_tag="P5201_11300"
     CDS             416..1609
                     /locus_tag="P5201_11300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012870329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dicarboxylate/amino acid:cation symporter"
                     /protein_id="HRV99172.1"
                     /translation="MKNLGLIPKLIIGIIVGILIGKFGGVLPVRILATFNGLFGNFLG
                     FVIPLIIIGFVAPGIGELEAGAGKLLGMTAGIAYSSTVMAGLAAFFVNSALFPLFLTP
                     GSIVLDDPGNPEHALLPSLFNVEMPPLMGVMTALLLAFTLGLGMAAIRSGALQRGFKE
                     FQQIIEKLIKGIIIPLLPFHIAGIFANMTHAGQVAEILSVFLKVFLIIIALHLSVLIV
                     QYFAAGLIGGKNPVRLLRTMLPAYLTALGTQSSAATIPVTLEQTVRNGVRRGVANFVV
                     PLCATIHMSGSTITITSCAMAIMVLNGISFSFSTMLGFIMMLGVTMVAAPGVPGGAVM
                     AALGLLQTMLGFDQTLISLMIALYVAQDSFGTACNVTGDGAIAVVVDRIYGKLAEDEP
                     SGAAS"
BASE COUNT          305 a          576 c          573 g          373 t
ORIGIN      
        1 cacccccgcc gacggcaagg ccttcgctct tggagaagtc gcgctgcttc gcggaacggt
       61 gcggaacgaa ggcgtgctgc cggtcgggaa catctcgctc ttcttctacg acggcaaccc
      121 cgacggagga ggcaagccca tcgccaacag aatcgtctcc ggaatcgtgc cgggcggcga
      181 gtactccgtc gtgctgacgc actccttcga cacgccgggg ccgcgcagcg tcttcatgag
      241 ggtggtcccc aaggttggcg acccccccga ggacaacacg gtgagcgtgg ccgtcaccgt
      301 gcagggcggc gactcgggcg gcggcggatg cgccgtcgga actgcgggcg catggagcgc
      361 gctgctcctg ataggactgg cgccgctggc gttcggaaga aaggagaggt aaggcatgaa
      421 gaatcttggt ctcatcccga aactgatcat cggcatcatc gtcggcattc tcataggcaa
      481 gttcggcggg gttcttcccg tgcggattct ggcaaccttc aacggtcttt tcggcaactt
      541 cctcggtttt gtcattccgc tcatcatcat cggctttgtt gctcccggca tcggcgagct
      601 ggaggccggc gcagggaagc tgctcggcat gacggctggc atcgcctatt cctcgaccgt
      661 catggcgggg ctcgccgcct tcttcgtgaa cagcgcgctc ttcccgctct ttttgacgcc
      721 ggggagcatc gtgctcgacg atccggggaa tccggagcac gcgctgctcc cctcgctctt
      781 caacgtcgaa atgccgccgc tgatgggcgt gatgacggcg ctgctgctgg cgttcaccct
      841 cggcctgggg atggcggcca tacgcagcgg cgcgctccag cgtgggttca aggagtttca
      901 gcagatcatc gagaagctca tcaaggggat catcattccg ctgctcccct tccacatcgc
      961 cggtatcttc gccaacatga cgcacgccgg gcaggtggcg gagatcctct cggtcttcct
     1021 gaaggtgttt ttgatcatca tcgcgcttca tttgagcgtg ttgatcgtcc agtactttgc
     1081 ggccggactc atcggcggca aaaacccggt cagactgctc cggacgatgc ttccagcata
     1141 tttgacggcg cttgggacac agtcttcggc ggcgaccatt ccggtgacgc tcgaacagac
     1201 tgtccgtaac ggggtccgca gaggcgtggc gaacttcgtc gtgcctctct gcgccaccat
     1261 tcacatgtcg gggagcacca tcaccatcac gagctgcgcg atggcgatca tggtgctcaa
     1321 cggcatctcc ttctccttct cgacgatgct cggcttcatc atgatgctcg gcgttacaat
     1381 ggtggccgct cccggagtgc cgggcggcgc cgtgatggct gctctggggc tgctgcagac
     1441 gatgctgggt ttcgaccaga cgcttatctc gctgatgatc gcgctctacg tggcgcagga
     1501 cagcttcggg acggcatgca acgtaacggg cgacggcgcg atcgcggtgg tggtcgacag
     1561 gatttacgga aagctcgcgg aggacgaacc gtcgggcgca gcctcctgat ccggtaaccg
     1621 ttcccacctc tacagaaaga acgaacgggc gcgtccaccg atgcgcccgt tcgttctttc
     1681 tccggcggtt cgtggttcga ccgcgacaat catgtcgtca gccagcaggt tccataggga
     1741 ttcagcacga cctccgcgac gccgcgctcc gtcctgtact cctcgttcgt gagcagatcg
     1801 cggcaacttc cgtcgaggcc gggaagg
//
LOCUS       DAOTIL010000604         1823 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminobacteriaceae bacterium isolate AMR_MDS_3026
            NODE_76176_length_1823_cov_2.389706, whole genome shotgun sequence.
ACCESSION   DAOTIL010000604 DAOTIL010000000
VERSION     DAOTIL010000604.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868050
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminobacteriaceae bacterium (wastewater metagenome)
  ORGANISM  Aminobacteriaceae bacterium
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae.
REFERENCE   1  (bases 1 to 1823)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1823)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:19:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,224
            CDSs (total)                      :: 3,198
            Genes (coding)                    :: 3,184
            CDSs (with protein)               :: 3,184
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1823
                     /organism="Aminobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_76176_length_1823_cov_2.389706"
                     /isolate="AMR_MDS_3026"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:3034609"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(16..>1823)
                     /locus_tag="P5201_11305"
     CDS             complement(16..>1823)
                     /locus_tag="P5201_11305"
                     /inference="COORDINATES: protein motif:HMM:NF021489.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="DUF2207 domain-containing protein"
                     /protein_id="HRV99173.1"
                     /translation="DSTGRAVTLGFSVKSALLGGREVPHTERSISDGVRVYLGDPDGR
                     APIGEQTYTLVYVVTGALGFFDEHDELYWNVTGNDWAFAIDEARFSLSLPGGASPAAV
                     EFFTGWQGARGREAQLLADGSVLTTSPLPAGEGLTVAYAWPKGIVSPPKEPFLRTFFA
                     RYHVHFLAALPLLLLFLYFLLWLRWGKDPPMPVVIPLFSPSPEKTPGFLRYVSRMGMD
                     DACFTAEILHLAVKGYIVIKKQEPEKKSDKALYSLQRTEKDPPGLPGAEKLLLAALFS
                     GERKEVTIRNESSVVLGDARKRLTKLFEKNGKGFFFKNTGIWCSGLFLLVVGWLLMSA
                     GGQGELAGASAFLTAAFAVVCSLGASESIASGKFRRYDGSDLLAFIVRRFIPDFLGNL
                     IILVVSGIVLAVLAAGALFFSGLWMVVVVLFELCAWVVSLLASAPLYVPLLSFATAVV
                     IVVFRELMTIRTPEGNRVLAEAEGLILYMGTAERRRFEMLNPPEETPEVFETLFPYAF
                     ALDVAETWAQRFDSILKERKYTPDWYEGGGSLAYFYRTAGFSAALTSSTFGSAGSASG
                     GVAGSSSRSTGSWFSGFGGGGFSGGGRGGGGGGGW"
BASE COUNT          393 a          588 c          552 g          290 t
ORIGIN      
        1 cgctcactgc ggtcttcacc agccgccgcc accgccgccg cctcgaccgc ctccggaaaa
       61 gccgccgccg ccgaacccgg agaaccagct tcccgtcgag cgcgaggacg aacccgcaac
      121 gcccccagac gcgcttcccg cgcttccgaa tgtgcttgac gtcaatgccg cggagaaacc
      181 ggcggttctg tagaagtacg ccaggctgcc tcccccttcg taccagtccg gcgtatactt
      241 ccgctccttg agaatactgt cgaaacgctg cgcccaggtc tccgcgacgt caagcgcgaa
      301 ggcgtagggg aagagagttt caaagacctc gggagtttcc tccggaggat tgagcatctc
      361 gaagcggcgc cgttcggcgg ttcccatgta gaggatcaac ccttcagcct ccgcaaggac
      421 gcggtttccc tccggcgtcc gtatcgtcat cagttcgcgg aagacgacga tcaccacggc
      481 cgtcgcaaaa gagagcagag gaacgtagag tggagccgac gcaagcaaag agacgaccca
      541 cgcgcacagt tcgaagagga cgacaaccac catccacagg cccgagaaga aaagcgcccc
      601 cgccgcgagc acggcgagca cgataccgga gacaacgagg atgatcaggt ttcccaaaaa
      661 atccgggatg aagcgccgga caataaacgc aagcaggtcg cttccgtcgt accggcggaa
      721 ttttccggag gcgatgctct cggaagcgcc cagggagcag acgaccgcga acgccgccgt
      781 caggaaggcc gaagcgcctg caagctcgcc ctgccctccc gcgctcatca gcagccagcc
      841 gaccacgagg aggaaaagcc ccgagcacca aataccggtg ttcttgaaaa agaacccttt
      901 cccgttcttc tcgaacagct tcgtcaggcg cttccgcgca tcgcccagaa caacgctgct
      961 ctcgttccgt atggtcacct ctttacgttc cccggaaaag agagcggcga gcagcagctt
     1021 ttcggctccc ggaagtccgg gagggtcctt ttccgtccgt tgcagcgaat acagcgcttt
     1081 gtccgacttc ttttcgggct cttgcttctt gatgacgatg taccccttca cggcgagatg
     1141 cagaatttcg gcggtgaagc aggcgtcgtc catgcccatt ctgctcacgt accgcagaaa
     1201 gcccggcgtt ttttccgggg acggggagaa gagcgggatg acgacgggca tcggggggtc
     1261 tttgccccag cggagccaga gcaggaaata gagaaacagg aggagaagcg gcagagcggc
     1321 cagaaagtgt acgtgatacc gagcgaaaaa cgtacgaaga aaaggctctt ttggggggct
     1381 cacgattcct ttgggccatg cgtacgcaac ggtaagcccc tcgcccgcgg gaagcggcga
     1441 cgtcgtgagg acggagccgt ccgccagcag ctgcgcctcc cgccctcgcg ctccctgcca
     1501 ccccgtgaag aactcgaccg cggcagggga cgcgccgccg ggcagtgaga gggagaatcg
     1561 ggcctcgtcg atcgcgaagg cccagtcgtt gccggtgacg ttccagtaga gctcgtcgtg
     1621 ctcgtcgaaa aagccgagcg ccccggtaac gacatagacg agcgtatacg tctgttcgcc
     1681 gataggcgct cttccgtccg gatcgccgag gtagacccga acgccgtccg aaatggagcg
     1741 ttccgtgtgc ggaacctccc gtccgccgag caaagcgctc ttcaccgaaa agccgagggt
     1801 gacggcgcgt ccagtcgagt ctt
//
LOCUS       DAOTIL010000605         1819 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminobacteriaceae bacterium isolate AMR_MDS_3026
            NODE_76418_length_1819_cov_3.257370, whole genome shotgun sequence.
ACCESSION   DAOTIL010000605 DAOTIL010000000
VERSION     DAOTIL010000605.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868050
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminobacteriaceae bacterium (wastewater metagenome)
  ORGANISM  Aminobacteriaceae bacterium
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae.
REFERENCE   1  (bases 1 to 1819)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1819)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:19:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,224
            CDSs (total)                      :: 3,198
            Genes (coding)                    :: 3,184
            CDSs (with protein)               :: 3,184
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1819
                     /organism="Aminobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_76418_length_1819_cov_3.257370"
                     /isolate="AMR_MDS_3026"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:3034609"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..980
                     /locus_tag="P5201_11310"
     CDS             <1..980
                     /locus_tag="P5201_11310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006301714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="solute carrier family 23 protein"
                     /protein_id="HRV99174.1"
                     /translation="TPVVIGPVIMAIGFSLAPTAIQFNAANYWPVSLLVVALIFFFSL
                     VSKNKYANIFAILSSIVLTYLLCLGLSKAGIFPEGHPAHISLANVEAAPWYRIDIIMP
                     WGTPKFSLLAFGALLAGFFAVMIESVGDYHSVSYASGLDDPDEKTISRGIGAEGLNCA
                     LSGLFGSVGTTSYTENIGLIGLTGVASRWVVRVGAILLILMSFVTKFGALVATMPSPI
                     IGGAYIALFGTIGALGIQVLMRADMGSQRNVLIVGFAFLMAMGLPGWVEKNQELFMNP
                     AYHQVLQTLGGMVWAILKTPMAVAGICAAFCDSLVPGTDEERGIGVKMK"
     gene            1139..>1819
                     /locus_tag="P5201_11315"
     CDS             1139..>1819
                     /locus_tag="P5201_11315"
                     /inference="COORDINATES: protein motif:HMM:NF012695.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate nucleotidyltransferase"
                     /protein_id="HRV99175.1"
                     /translation="MNPVFTLVLAGGSGTRLWPLSREEFPKQFLRIEEEGSLYQCTLR
                     RAFAIGDPSRTKVLSSVRFGAMALLQARELLDVPQNFVVEEPCPKNTAPAVALGLLAL
                     MEECGATRESVVFVCPSDHIIREPGVFEEAVRTASAAARNGNVCVFGIPPTRPDTGFG
                     YVRAGGPCGEWRKVERFVEKPSLEKAEEYLEAGDYFWNGGMFIFTVGTMLDALAEHLP
                     ETAPLIEKG"
BASE COUNT          311 a          596 c          529 g          383 t
ORIGIN      
        1 tcacgcctgt ggtcataggc ccggtcatca tggccatcgg cttttccctt gctcctacgg
       61 ccatccagtt caacgccgcc aactactggc ccgtttcgct gctcgtcgtg gcgctcatct
      121 tcttcttcag cctcgtgagc aagaacaagt acgccaacat cttcgcgatc ctctcgtcga
      181 tcgtgctcac ctacctcctc tgcctcggac tttccaaagc gggaatcttc cccgagggac
      241 accctgcgca tatcagcctt gcgaacgtcg aggcggcccc ctggtaccgc atcgacatca
      301 tcatgccgtg gggaacgccc aagttcagcc tcctggcgtt cggagcgctg cttgccggat
      361 tcttcgccgt catgatcgag tccgtcggcg actaccactc ggtctcctac gcctccggcc
      421 ttgacgaccc cgatgagaag accatcagcc gcggtatcgg cgcggagggc ttgaactgcg
      481 ccctctccgg tctcttcggc tccgtgggca ccacctccta cacggagaat atcggcctca
      541 tcggccttac cggagtggcc tcccgctggg tcgtccgcgt cggcgccatc ctgctgattc
      601 tcatgagctt cgtgaccaag ttcggcgcgc tcgtcgcgac catgccctct ccgattatcg
      661 gcggcgcgta catcgccctg ttcggcacca tcggcgcact cggcatccag gtgctcatgc
      721 gcgccgacat gggcagccag cgcaacgtcc tcatcgtcgg cttcgccttc ctgatggcga
      781 tggggcttcc cggctgggtc gagaagaacc aagagctctt catgaacccc gcctaccatc
      841 aggtgttgca gacgctcggc ggcatggtct gggccattct gaagaccccc atggcggttg
      901 caggaatctg cgcggccttc tgcgactccc ttgttccggg aacggacgag gagcgcggca
      961 tcggcgtcaa gatgaagtaa cccgattctc cctttttgac gcgctgaaaa atcccccctg
     1021 cgtagggggg atttttgttg tcctcttgct gcgaaacctc cccggttcta tactttccga
     1081 tctccggaat gctatactct caaaatagaa tcacataccg acgaatggac gtgattgaat
     1141 gaatcccgta ttcacccttg tgctcgcagg cggatcggga accaggcttt ggcctctctc
     1201 ccgggaggag ttccccaagc agttcctgcg gattgaagag gagggcagtc tctaccagtg
     1261 caccctgcgg cgggcgtttg cgatcggcga tccctcgcgg acaaaagtac tctcgagcgt
     1321 caggttcggc gcgatggcgc tcctgcaagc gcgtgagctt ctggacgttc cgcagaactt
     1381 tgtcgtggag gagccctgcc cgaagaacac agctccggca gtcgcgctcg ggctgctcgc
     1441 cctgatggag gagtgcggag cgaccagaga gagcgttgtc ttcgtctgtc ccagcgacca
     1501 tatcatccgc gagccgggtg ttttcgagga agccgtccgt accgcctccg ctgcggcgcg
     1561 caacgggaac gtatgcgtct tcggcattcc cccgaccagg cccgacacag gcttcggcta
     1621 cgtccgggcg ggcgggccgt gcggcgaatg gcggaaggtg gagcgtttcg tcgagaagcc
     1681 ctctttggag aaggcggagg agtacctcga agcgggagac tacttctgga acggcggtat
     1741 gttcatcttc accgtcggca ctatgctcga cgcgctcgcc gaacacctgc cggagacggc
     1801 tcccttgatc gaaaagggg
//
LOCUS       DAOTIL010000606         1819 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminobacteriaceae bacterium isolate AMR_MDS_3026
            NODE_76423_length_1819_cov_3.132086, whole genome shotgun sequence.
ACCESSION   DAOTIL010000606 DAOTIL010000000
VERSION     DAOTIL010000606.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868050
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminobacteriaceae bacterium (wastewater metagenome)
  ORGANISM  Aminobacteriaceae bacterium
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae.
REFERENCE   1  (bases 1 to 1819)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1819)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:19:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,224
            CDSs (total)                      :: 3,198
            Genes (coding)                    :: 3,184
            CDSs (with protein)               :: 3,184
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1819
                     /organism="Aminobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_76423_length_1819_cov_3.132086"
                     /isolate="AMR_MDS_3026"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:3034609"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..553)
                     /locus_tag="P5201_11320"
     CDS             complement(<1..553)
                     /locus_tag="P5201_11320"
                     /inference="COORDINATES: protein motif:HMM:NF012760.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016742 - hydroxymethyl-, formyl- and
                     related transferase activity [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formyltransferase family protein"
                     /protein_id="HRV99176.1"
                     /translation="MSNLRAAVFAYSEVGHTCLKELLRQGTSVQAVFTHEDNPAETLW
                     FPSVAALAREAGIPVFTPDSLGDEEYAMLLSLRPDVIFSFYYRSMIPERFLAIPPKGA
                     FNMHGSLLPKYRGRACVNWAVLNGETVTGATLHHMVRRADAGDIVDREAVPILPDDTA
                     LDVSRKVAEAAGIILERTLPSIAL"
     gene            complement(546..1484)
                     /locus_tag="P5201_11325"
     CDS             complement(546..1484)
                     /locus_tag="P5201_11325"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008709292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HRV99177.1"
                     /translation="MSESCVLSVIVPVYNEEESLPYLCPRLLKTLDGMHRPYEIIFVN
                     DGSRDASLKMLLDFHKANPGKVRVVDFNGNFGQHMAIMAGFDHSRGDVIVTLDADLQN
                     PPEEIPNLVAKIDEGHDVVGTIRQNRQDPKFRKMASRMVNAITNKITGLRLNDYGCML
                     RAYRRDIVNIINQSCESTTFIPALAQKFAVNPVEIPVGHTEREHGTSKYSIFRLIRLN
                     FDLMTGFSLFPLQAVTMIGIVVSALSFVFALYLLLRRIIVGPEAEGVFTLLNINFFLM
                     GITMSCVGITGEYIGRIYQEVRKRPRYVIRREWNDE"
     gene            complement(1583..>1819)
                     /locus_tag="P5201_11330"
     CDS             complement(1583..>1819)
                     /locus_tag="P5201_11330"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRV99178.1"
                     /translation="RVVLVNYMGELEFGALQEADPSWFIDGKGLAELWTGDRRVILVV
                     NSDHKEYVAQQTGLSLPEPLAETAKRLVFVNRKE"
BASE COUNT          331 a          535 c          594 g          359 t
ORIGIN      
        1 cgagggcgat tgaggggagc gtccgctcca ggatgatccc tgcggcttcg gccactttcc
       61 gggagacgtc gagggccgta tcgtcgggga ggatgggcac agcctcccgg tcgacgatat
      121 cccccgcgtc ggctcgtcgt accatgtggt ggagcgtcgc gccggttacc gtctcgccgt
      181 tgaggacggc ccagttgacg caggcccgtc ccctgtactt cggcagcagc gagccgtgca
      241 tgttgaaggc ccccttcggc gggatcgcga ggaagcgctc ggggatcatc gagcggtagt
      301 agaaagagaa gatgacgtcg gggcgcagag agagcagcat cgcgtactcc tcgtcgccga
      361 gagagtccgg ggtgaagacg gggattcccg cttcccgcgc gagcgccgcc accgagggga
      421 accagagcgt ttccgccggg ttgtcttcgt gggtgaagac cgcctgaacc gacgtcccct
      481 gacgcagcag ctccttcagg catgtgtgcc cgacctcgct gtaggcgaag acggcggcgc
      541 ggaggttact catcgttcca ctccctgcgg atgacgtagc gggggcgttt gcggacctcc
      601 tggtagatgc gtccgatata ctctcccgtg atgccgacgc aggacatggt gatgcccatc
      661 aggaaaaagt tgatgttgag cagcgtgaag acgccctccg cttccgggcc gacgatgatt
      721 ctgcggagca gcaggtagag tgcgaagacg aagctcagcg cggagacgac gatgccgatc
      781 atcgtcaccg cctgaagcgg gaagagagag aaccccgtca tcaggtcgaa gttgagccgg
      841 atgaggcgga agatgctgta cttcgacgtg ccgtgctccc gctccgtatg ccccacaggg
      901 atttcgaccg ggttgacggc gaacttctgc gccagcgcgg ggatgaaggt cgtgctctcg
      961 cagctctggt tgatgatgtt gacaatatct cgccggtagg cgcggagcat gcagccgtag
     1021 tcgttcaggc gaagccccgt gatcttgttg gtgatggcgt tgaccatgcg cgaagccatc
     1081 ttgcggaact tcggatcctg gcggttctgg cggatcgttc ccacgacgtc gtgcccctcg
     1141 tcgatcttcg ccacaagatt tggtatctct tcgggcgggt tctggaggtc ggcgtcgagc
     1201 gtcacgatga cgtctccccg cgaatgatcg aatccggcca tgatcgccat gtgctggccg
     1261 aagttgccgt tgaagtcgac gacgcgcacc ttccccggat tggccttgtg aaagtcgagc
     1321 agcatcttca gggaggcgtc cctgcttccg tcgttgacga agatgatctc gtagggacga
     1381 tgcattccat cgagcgtctt cagcagcctc gggcagagat aggggagaga ctcctcctcg
     1441 ttgtagaccg gtacgatgac agagagaacg catgattcgg acattgttat ttcccttctc
     1501 cttccgggat ggttgctcat gaagagcaaa tactcccacc cgggcgccgc ccgggtgcta
     1561 ccatgtaccg cgccggtgcg ggttattcct tgcggttgac gaagacgagg cgctttgcag
     1621 tctcggctaa aggttccgga agcgaaagcc cagtctgctg cgcgacatac tccttgtggt
     1681 cgctgttgac gaccaggatg acgcgccggt cgcccgtcca aagctcggcg agccccttcc
     1741 cgtcgatgaa ccatgacgga tcggcctcct gaagcgcgcc gaactcgagt tcccccatgt
     1801 agttgaccag cacgacccg
//
LOCUS       DAOTIL010000607         1818 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminobacteriaceae bacterium isolate AMR_MDS_3026
            NODE_76487_length_1818_cov_3.059558, whole genome shotgun sequence.
ACCESSION   DAOTIL010000607 DAOTIL010000000
VERSION     DAOTIL010000607.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868050
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminobacteriaceae bacterium (wastewater metagenome)
  ORGANISM  Aminobacteriaceae bacterium
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae.
REFERENCE   1  (bases 1 to 1818)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1818)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:19:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,224
            CDSs (total)                      :: 3,198
            Genes (coding)                    :: 3,184
            CDSs (with protein)               :: 3,184
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1818
                     /organism="Aminobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_76487_length_1818_cov_3.059558"
                     /isolate="AMR_MDS_3026"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:3034609"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..982)
                     /locus_tag="P5201_11335"
     CDS             complement(<1..982)
                     /locus_tag="P5201_11335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008522569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinylglutamate desuccinylase"
                     /protein_id="HRV99179.1"
                     /translation="MNIRTAKLIILLFAGSCAAIAGSLFKEHMEYKEAVVASPFLSEV
                     RKLSDYSDAVKGSVNDANVYIFNGPTPGGTVVLLGGTHPEEPVANLSAQIFTENVHPV
                     QGRVIVINRINTSASMATRMGEAYPRFYHISTPWGVKKWRYGDRLATPLHSWPDPEVY
                     VHYPSGQNLAYMDVRNANRTWPGRPDGLLCERTNYALMELIRKEKADIVMDFHEAELE
                     YAVENTIVVHEKGQSVAAMASMMLTSQTFDVPIGMEFSPKALHGLSHREVGDHSDAVS
                     YLVEVAEPMLDRIRGITDEELLMSGKDRFVMKAGEYKLLYSPIDESGWPVE"
     gene            complement(999..1169)
                     /locus_tag="P5201_11340"
     CDS             complement(999..1169)
                     /locus_tag="P5201_11340"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRV99180.1"
                     /translation="MDMLTINSVVMILYYFITTFFLLAMVRNFIVTRDVQEALLYAVV
                     MIPFVLRLLRLK"
     gene            complement(1175..>1818)
                     /locus_tag="P5201_11345"
     CDS             complement(1175..>1818)
                     /locus_tag="P5201_11345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008520309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="C4-dicarboxylate ABC transporter"
                     /protein_id="HRV99181.1"
                     /translation="GWNWRRAGAPFLVLAALVLAGRAFPYSFPVLGLPLIFMISALAV
                     FVISPSRLPLFRVAQHTVKILLGLVSIMVVVGVLIQVMTLSGVRGLLSLAVVTLPLSV
                     LFFTLWMILPISEGMLQYAVAPLFGVPLIMLFNMKGLDPVVSLAAWSVMWPLGDCLPP
                     TAVVGRAAVMELDFEGDYYRGFVKTALPVMAFILLLSTLFMVYSKEISQVIGG"
BASE COUNT          479 a          471 c          488 g          380 t
ORIGIN      
        1 tttccacagg ccatccgctc tcatcgatgg gggagtagag gagcttgtat tctcccgcct
       61 tcatgacaaa ccggtccttt cctgacatca agagctcttc gtcggtgatc ccgcgaattc
      121 tgtcaagcat aggttccgcc acttcaacaa ggtaagaaac agcgtcggaa tgatctccga
      181 cctcgcggtg agaaagaccg tgaagcgctt ttggggagaa ctccatacca ataggtacgt
      241 caaaagtctg ggaggtgagc atcatagatg ccatggctgc taccgattgt cccttttcgt
      301 gaacgacgat ggtgttctcc acggcgtatt caagctcggc ttcgtggaag tccatgacga
      361 tatccgcttt ttctttacgg atcagttcca tgagcgcgta attcgtacgt tcgcaaagga
      421 ggccgtcggg gcgtcccggc catgtccggt tcgcgttccg tacatccatg taggcgagat
      481 tctgtccgct cgggtagtga acgtacacct cggggtccgg ccaggagtgc aacggggttg
      541 cgagccggtc gccatatctc catttcttta ctccccaggg ggtggaaata tgataaaacc
      601 tggggtacgc ctctcccatt cgtgtggcca tggaggcgct tgtgttgatg cggttgatga
      661 cgatgacccg tccttgaacg ggatgaacgt tctcggtaaa tatctgagct gaaaggttcg
      721 caaccggctc ttccgggtga gttcctccga gcaagacaac ggtcccgccg ggggtcggac
      781 cgttgaaaat atatacattc gcatcgttaa ccgagccttt gactgcatcg ctgtaatcgc
      841 tgagttttct gacttccgag agaaaggggc tcgcgacgac agcttcttta tactccatat
      901 gctctttaaa aagagatcct gcgatcgcag cgcaagagcc tgcgaaaaga agaatgatga
      961 gttttgcagt gcgaatattc attctctaag gcccctttct acttgagccg cagaaggcgc
     1021 aaaacaaagg gaatcataac gacggcgtac aggagagcct cttgaacatc ccgtgtcacg
     1081 atgaagtttc gaaccatcgc aagaagaaaa aaagttgtga tgaagtaata caggatcatc
     1141 accactgaat ttatcgtaag catatccaca gacgtcaacc tccgatcact tgggaaatct
     1201 ccttgctgta caccatgaag agagtggaca ggaggagaat aaaagccatc accggaagag
     1261 ctgttttgac aaacccccgg taatagtctc cctcgaagtc aagctccata acagcggcac
     1321 gtccgactac cgcagtaggc ggaagacagt cgcccagcgg ccacatgacc gaccacgctg
     1381 caagggaaac tacgggatca agccctttca tgttgaacag catgatcaaa ggaacgccga
     1441 agagcggggc gactgcgtac tgaagcattc cctccgatat gggcagtatc atccagagag
     1501 taaagaaaag cacgctgagc ggaagcgtta ctacggcaag agagagaagc ccccgaacgc
     1561 cgctgagagt catcacctga ataagaacgc ctacaaccac catgatactc acaagtccga
     1621 gcaggatttt tacagtatgc tgcgccacac ggaacaaggg aaggcgagag ggactgataa
     1681 cgaagactgc gagggcgctt atcataaaaa tcagcggcag tcccagaacc gggaatgaat
     1741 aggggaacgc tcttccggcg agaacgagtg ccgcgaggac gagaaaagga gcgcccgcac
     1801 ggcgccaatt ccagcctt
//
LOCUS       DAOTIL010000608         1815 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminobacteriaceae bacterium isolate AMR_MDS_3026
            NODE_76667_length_1815_cov_3.520455, whole genome shotgun sequence.
ACCESSION   DAOTIL010000608 DAOTIL010000000
VERSION     DAOTIL010000608.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868050
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminobacteriaceae bacterium (wastewater metagenome)
  ORGANISM  Aminobacteriaceae bacterium
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae.
REFERENCE   1  (bases 1 to 1815)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1815)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:19:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,224
            CDSs (total)                      :: 3,198
            Genes (coding)                    :: 3,184
            CDSs (with protein)               :: 3,184
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1815
                     /organism="Aminobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_76667_length_1815_cov_3.520455"
                     /isolate="AMR_MDS_3026"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:3034609"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..233
                     /locus_tag="P5201_11350"
     CDS             <1..233
                     /locus_tag="P5201_11350"
                     /inference="COORDINATES: protein motif:HMM:NF018513.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="TRAP transporter large permease subunit"
                     /protein_id="HRV99182.1"
                     /translation="ISPVHLGVVVVMTLAVGLVTPPYGLCLLIAGSIADLSIERSLKG
                     VLPYISALMIILLLVAMFPDVAFFVPKALGVM"
     gene            complement(316..1383)
                     /gene="buk"
                     /locus_tag="P5201_11355"
     CDS             complement(316..1383)
                     /gene="buk"
                     /locus_tag="P5201_11355"
                     /EC_number="2.7.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005661081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047761 - butyrate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006113 - fermentation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="butyrate kinase"
                     /protein_id="HRV99183.1"
                     /translation="MKILALNPGSTSTKVALFEDHTELWSETQRYDTDVIGRFSGVME
                     QEEFRLEEIRKCLAAHGATVADLDAVVGRGGLLKPIESGTYEVSDKMLEDLRACTWGA
                     HASNLGAPLAVRLADEGGCGKAYIVDPVVVDEMCPLARYSGLPEIERRSIFHALNQKA
                     VARRAAAELQKPYAECNLVVAHMGGGVSVGAHEKGRVIDVNNALDGDGPFSPERAGSL
                     PVGGLVKLAFSGKYELPQLLKMLTGKGGLVAHLGTNDLREVIRRMDDGDDNARLLFEA
                     LAYRIAKEIGACAASLSGAVDAIILTGGLAYNERFTKLISDRVAFIAPVKIYPGEDEM
                     QALVEGALRVLNGSEEARRYE"
     gene            complement(1397..>1815)
                     /locus_tag="P5201_11360"
     CDS             complement(1397..>1815)
                     /locus_tag="P5201_11360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008520894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="phosphate acyltransferase"
                     /protein_id="HRV99184.1"
                     /translation="RSFGVAEPKVAVLAAVEVVNPDMPATLDAAALTQMNRRGQIKNC
                     IVDGPFALDNAVSEESAKTKGIVSPVAGKADIFIVPNIDAGNMLAKGIVYFSKNETAG
                     LILGASAPIVLTSRADSTRAKLLSIAAAVTLSDFNK"
BASE COUNT          300 a          582 c          561 g          372 t
ORIGIN      
        1 gcatctctcc cgtccatctc ggcgtcgtgg tcgtcatgac gctcgcggtc ggtcttgtga
       61 cacccccgta cgggctctgt ctgctcatcg cgggttccat cgcagatctg tctatcgaac
      121 gttctctgaa aggcgtcctt ccctacatct ccgcgctgat gatcatcctg ttgctggtgg
      181 cgatgttccc cgacgtcgcg ttcttcgttc ccaaagctct cggcgtgatg tagtccccga
      241 atgcgaagag agggagacgg aaaaatttcc gtcctccctc tcatttttta tatttcggta
      301 cccggcagga aaagtctatt cgtaccggcg agcctcttcc gagccgttca ggacgcgaag
      361 cgccccttcg acgagggcct gcatctcgtc ctctccgggg taaatcttca ccggggcgat
      421 aaaggcgacc cggtcgctta tgagcttggt gaatcgctcg ttgtacgcaa gccctcccgt
      481 aagaatgatg gcgtccactg ctccggagag cgaagcggcg cacgcgccga tctctttcgc
      541 aatcctgtag gcaagcgcct cgaaaaggag acgcgcattg tcgtccccgt cgtccatccg
      601 gcgaatcacc tcacggagat cgttcgtccc aaggtgcgcg acaagaccac ctttccccgt
      661 gagcatcttc agcagctgcg gcagctcgta cttgccgctg aacgccagct tgaccagccc
      721 gccgacgggg agactcccgg cccgctcggg cgagaagggg ccgtcgccgt cgagcgcgtt
      781 gttcacgtcg atcacgcgcc ccttttcatg cgcgcccacg gagacgccgc cgcccatgtg
      841 cgccacgacg aggttgcact ccgcgtacgg tttttgcagc tccgcggcgg ctcttctggc
      901 cacggccttc tggttgagcg cgtggaagat ggagcggcgc tcgatctccg gaagtccgga
      961 gtagcgtgca agcggacaca tttcgtccac gacgaccggg tcgacgatgt acgccttccc
     1021 gcatccgccc tcgtcggcca gacgaacagc cagcggagcg cccaggttgc tcgcgtgcgc
     1081 gccccacgta caggcgcgca ggtcttcgag catcttatcg ctcacttcgt acgtgccgct
     1141 ctcgatcggc ttgagaagtc ctccccgccc cactaccgcg tcgaggtccg cgaccgttgc
     1201 gccgtgcgcg gcaaggcact tgcggatctc ttcaaggcgg aattcctcct gctccatgac
     1261 gccggagaaa cggccgatca cgtccgtatc gtagcgctgc gtttcgctcc aaagttcggt
     1321 atggtcctcg aagagagcga ccttggtgct ggtggagccg ggattgagag cgaggatttt
     1381 cataacgacg aacgctctac ttattaaagt cggagagcgt gaccgccgcg gcgatggaga
     1441 gcagcttcgc ccttgtcgag tcggcgcggc tggtgaggac gatgggggcg gaggcgccga
     1501 ggatgagtcc ggccgtctcg ttcttcgaga agtagacgat ccccttcgcg agcatgttcc
     1561 ccgcgtcgat gttcgggacg atgaagatgt cggcctttcc ggcgacgggg gagacgatcc
     1621 ccttcgtctt ggcggactcc tcgctcaccg cgttatcgag cgcgaacggg ccgtcgacga
     1681 tgcagttctt tatctggccg cggcggttca tctgcgtgag cgcggcggcg tcaagggtgg
     1741 cgggcatgtc ggggttcacc acttcgacgg cggcgaggac ggcgaccttg ggctcggcaa
     1801 cgccgaacga acggg
//
LOCUS       DAOTIL010000609         1815 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminobacteriaceae bacterium isolate AMR_MDS_3026
            NODE_76675_length_1815_cov_3.205114, whole genome shotgun sequence.
ACCESSION   DAOTIL010000609 DAOTIL010000000
VERSION     DAOTIL010000609.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868050
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminobacteriaceae bacterium (wastewater metagenome)
  ORGANISM  Aminobacteriaceae bacterium
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae.
REFERENCE   1  (bases 1 to 1815)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1815)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:19:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,224
            CDSs (total)                      :: 3,198
            Genes (coding)                    :: 3,184
            CDSs (with protein)               :: 3,184
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1815
                     /organism="Aminobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_76675_length_1815_cov_3.205114"
                     /isolate="AMR_MDS_3026"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:3034609"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(43..762)
                     /locus_tag="P5201_11365"
     CDS             complement(43..762)
                     /locus_tag="P5201_11365"
                     /inference="COORDINATES: protein motif:HMM:NF013168.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-(5-phosphoribosyl)-5-[(5-
                     phosphoribosylamino)methylideneamino]
                     imidazole-4-carboxamide isomerase"
                     /protein_id="HRV99185.1"
                     /translation="MIVFPAMDLYGGEIVRLRQGKFSDRTVYPAKIAETAKSFLEAGS
                     EWIHLIDLQGAEEGAPRHLSVVGRLKETGLLVQYGGGLRSEEDIAAAFEAGADRLYLG
                     SILVKDRFLAGRMYDAYGSKVIPAVDIRNGDVVISGWKEAGGIAPEALIERLRAIGYR
                     LFLVTAVHRDGMETGPDREMYRRLLVRFPDIGFIAAGGLSSLEDVRHLKEDGLAGAVL
                     GRSLYEGSILLKDALREGASC"
     gene            complement(759..1382)
                     /gene="hisH"
                     /locus_tag="P5201_11370"
     CDS             complement(759..1382)
                     /gene="hisH"
                     /locus_tag="P5201_11370"
                     /EC_number="4.3.2.10"
                     /inference="COORDINATES: protein motif:HMM:TIGR01855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000107 - imidazoleglycerol-phosphate
                     synthase activity [Evidence IEA];
                     GO_function: GO:0016884 - carbon-nitrogen ligase activity,
                     with glutamine as amido-N-donor [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="imidazole glycerol phosphate synthase subunit
                     HisH"
                     /protein_id="HRV99186.1"
                     /translation="MIAVVDYQVGNTGNVRRAFERLGEQVCLLHSPDELGDRVSVAVL
                     PGVGAFGPASERLAASGWRETLQSWARSGRPLLGICLGMQLLCEKGLEDGEYEGLGLI
                     PGTALPLAASKIPHMGWNTVEWTGRPPSFLPSAPDGSFFYFVHSFALPEGEFTTGRTT
                     LEGNVFSSMVLKGSVAGFQFHPERSGPEGVALLGETVRFLRKEGAPQ"
     gene            complement(1379..>1815)
                     /locus_tag="P5201_11375"
     CDS             complement(1379..>1815)
                     /locus_tag="P5201_11375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013048082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="imidazoleglycerol-phosphate dehydratase"
                     /protein_id="HRV99187.1"
                     /translation="GIGLDVTATGDVDVDCHHVTEDIGILLGKAFEQARSGRNIQRYG
                     WCALPMDGSLVLAAVDFSGRGQFEWKGAFPAERCGDFDLELLPEFWRAVCREGRITLH
                     AQALACDNAHHLAEALFKGAGKVFRQALSPAGELQSTKGTLA"
BASE COUNT          326 a          611 c          553 g          325 t
ORIGIN      
        1 cgcgcccccc gagcacgtcg aggcacggga tgattcgcct cgtcagcatg aagcgccctc
       61 tcggagcgcg tccttcagga ggatgcttcc ttcgtagagc gatctgccga ggacggcgcc
      121 cgcgagcccg tcctccttca aatgccgtac gtcctccagc gaggagagcc cccccgcggc
      181 gatgaacccg atgtccggaa aacggacgag caggcggcgg tacatctccc tgtcggggcc
      241 tgtctccatg ccgtcacggt gcaccgcggt gacgaggaaa agacggtatc ctatcgcccg
      301 tagcctttcg atcagcgctt ccggggcgat gccgcccgcc tctttccagc cggagatcac
      361 cacatcgccg ttgcggatgt ctaccgcggg gatgaccttc gacccgtagg cgtcgtacat
      421 cctgcccgcg aggaaacgat ccttcacgag gatgcttccc agatagagcc tgtcggcgcc
      481 cgcctcgaag gctgcggcga tgtcctcctc ggatcggagt cctccgccgt actgaacaag
      541 gagccctgtt tccttgagac ggccgacaac ggaaaggtgg cgcggcgctc cctcctcggc
      601 gccttgcagg tcgatcaggt gaatccattc cgaccctgct tcaaggaagc tcttcgccgt
      661 ctcggcgatc tttgccggat acacggttct gtcgctgaac tttccctgcc tgaggcggac
      721 gatttcgcct ccgtagagat ccatcgcggg aaaaacgatc attgcggggc gccctccttt
      781 cgtagaaacc tgacggtctc ccccagcagc gcgacgcctt cggggccgct gcgctccggg
      841 tgaaactgga atcccgccac ggagcccttc agcaccatcg acgagaagac atttccttcg
      901 agcgttgtcc gccctgtcgt gaattcacct tcggggagcg cgaagctatg gacgaagtag
      961 aagaacgagc cgtcaggcgc ggacggtaaa aacgacggcg gtcgaccggt ccattctaca
     1021 gtgttccacc ccatgtgagg aattttggac gctgcaaggg ggagcgcggt tcccgggatc
     1081 agtccgaggc cttcatattc cccgtcctca agcccctttt cgcacagaag ctgcatcccg
     1141 aggcagatgc cgagcagcgg ccgcccggaa cgcgcccacg attgaagtgt ttcccgccac
     1201 cccgaggctg cgagtcgttc cgaagccggt ccgaatgccc cgactcccgg aagcacggcg
     1261 accgaaacgc ggtcgccgag ttcgtccgga gagtggagca gacagacctg ctccccgagg
     1321 cgctcgaagg cgcggcggac attacccgtg tttcccacct ggtagtccac gacggcgatc
     1381 atgcgagcgt tcctttcgtg ctttgcagct ctccggcggg agagagggcc tgccggaaca
     1441 ccttgcctgc tcccttgaag agcgcctctg caagatggtg tgcgttgtcg caggcgaggg
     1501 cctgcgcgtg gagcgtgatc cttccttcgc ggcagaccgc gcgccagaac tcgggcagaa
     1561 gctcaaggtc gaagtcgccg caccgctcgg cagggaacgc tcccttccat tcgaactgcc
     1621 cccggccgct gaaatcgacg gcggccagca caagagagcc gtccatggga agagcgcacc
     1681 agccgtagcg ctgaatgttc cggccggaac gggcctgttc gaacgccttt cccagcagaa
     1741 tgcctatgtc ttcggtcacg tggtgacagt ccacgtcgac gtctccggtc gccgtcacgt
     1801 cgaggccgat cccgg
//
LOCUS       DAOTIL010000610         1814 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminobacteriaceae bacterium isolate AMR_MDS_3026
            NODE_76754_length_1814_cov_2.439454, whole genome shotgun sequence.
ACCESSION   DAOTIL010000610 DAOTIL010000000
VERSION     DAOTIL010000610.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868050
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminobacteriaceae bacterium (wastewater metagenome)
  ORGANISM  Aminobacteriaceae bacterium
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae.
REFERENCE   1  (bases 1 to 1814)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1814)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:19:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,224
            CDSs (total)                      :: 3,198
            Genes (coding)                    :: 3,184
            CDSs (with protein)               :: 3,184
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1814
                     /organism="Aminobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_76754_length_1814_cov_2.439454"
                     /isolate="AMR_MDS_3026"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:3034609"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..706)
                     /gene="yedE"
                     /locus_tag="P5201_11380"
     CDS             complement(<1..706)
                     /gene="yedE"
                     /locus_tag="P5201_11380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005658619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YedE family putative selenium transporter"
                     /protein_id="HRV99188.1"
                     /translation="MKGFNNLLMSRFGPAITGGVVGALAALLVYFGNPGNMGICVACF
                     TRDIAGALGLHRAGVVQYLRPEIPGFILGSFFAAFLAKEYRPRSGSSPIVRFFLGFFA
                     MIGALIFLGCPWRAYARLAGGDWNALAGIGGLIVGIGIGIVFLWQGFSLGKAEKSPKW
                     AGAVMPLAALALLALLFFRPLFGPEGTGPVFFSKSGPGAAVAPVAIALGAGLLIGWMA
                     QKSRFCTVGAIRDLIML"
     gene            1092..1541
                     /locus_tag="P5201_11385"
     CDS             1092..1541
                     /locus_tag="P5201_11385"
                     /inference="COORDINATES: protein motif:HMM:NF020400.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CGGC domain-containing protein"
                     /protein_id="HRV99189.1"
                     /translation="MSSDIVRIGIFICNRYHTCAGGKCLRSMRNREGAFSIYKDREVE
                     LVGYTTCDGCPGGNIEYAPAEMKKNGATVIHLATGMVVGYPPCPRIDYFRRYINTAFD
                     LPVVVGTHPIPEKYFVIHEALGTWNSPMWQELIAPTLATPEIRLAYN"
     gene            complement(1645..1803)
                     /locus_tag="P5201_11390"
     CDS             complement(1645..1803)
                     /locus_tag="P5201_11390"
                     /inference="COORDINATES: protein motif:HMM:NF015050.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRV99190.1"
                     /translation="MALLSLGVKGIRLGPTLPGFLSPNVAGVLVKNFDIKPISTVEED
                     VAAMMAGK"
BASE COUNT          398 a          557 c          547 g          312 t
ORIGIN      
        1 tcagcatgat caggtcgcgg atcgcgccca cggtgcagaa acggctcttc tgcgccatcc
       61 agccgataag cagccccgcg ccgagagcga ttgcgaccgg agcgacagcg gcgcccggcc
      121 cggatttgga gaaaaagacc ggtccggttc cttcggggcc gaagagcggc ctgaaaaaga
      181 gaagggcaag cagcgcgagc gcggcgagcg gcatgacggc ccccgcccac ttgggcgact
      241 tctccgcctt gccgaggctg aatccctgcc acagaaagac gatgccgatt ccgatgccga
      301 cgatcagtcc gccgatgccc gcaagcgcgt tccagtcgcc gcctgcgaga cgggcgtacg
      361 ctctccacgg gcagccgagg aagatcagcg ccccgatcat cgcgaagaag ccgaggaaga
      421 agcggacgat gggggaggag ccggaacgcg gacggtactc cttcgccagg aacgcggcga
      481 agaacgaacc cagaatgaag ccgggaatct cgggacggag gtactggacc acgcccgccc
      541 tgtgcagtcc gagggctccc gcgatatcgc gcgtgaagca cgccacgcag atgcccatgt
      601 ttcccggatt gccgaagtag accagcagcg ccgcaagcgc gcccacgacg cccccggtga
      661 tcgcggggcc gaagcgggac atgagaagat tgttgaaacc cttcatgaag aacactcacc
      721 ctttcgaatc aaatacggaa taaattgaat tcctcagagt gagattataa ttcgtattcc
      781 agattagcgc aaggaagatt tttccccgac aggcgcagga agctttccct gttcctcaaa
      841 aaaaccttct gcgggcacac aagttccgcg ggcttttcca cgaacagacc tcgggatctc
      901 tttaccagcg tctctccgcg tccacgagat tcctccggca aattccattg ttcctgctgc
      961 gtccgccgaa ggacgcactc tctttttgaa atgctcgggg ttgcaaatcg gcgggggagg
     1021 tttatagtaa tgagaacaga tatcaacaag ctggccgtac ggccggaatt tcgacccgag
     1081 gaggcgtttt catgagcagc gacatcgtcc ggataggtat tttcatctgt aaccgctatc
     1141 acacgtgcgc ggggggaaag tgcctgcgct ccatgcgcaa cagggagggt gcgttcagta
     1201 tctataagga cagagaggta gaactcgtcg gctacaccac ctgcgacggc tgtcccggcg
     1261 gcaacatcga gtacgccccc gccgagatga agaagaacgg cgcgaccgtg atccacctcg
     1321 ccacgggcat ggttgtgggc tacccgccct gtccccggat cgactacttc cgccgctata
     1381 tcaacacggc attcgatctt ccggtggtcg tgggcacgca cccgattccg gagaagtact
     1441 tcgtcatcca cgaggcgctt ggaacgtgga actccccgat gtggcaggag ctgatcgccc
     1501 cgacgctcgc caccccggag atccgcctgg cgtacaacta gggggcgggc gaaacatttt
     1561 cagcacaaag agcgggagga tcacgccttt tcgaagggcg atcctcccgc tcttatcatt
     1621 tttggcgcgc ttgcgcctcc cggactactt gcccgccatc atcgcggcga cgtcttcctc
     1681 caccgtcgag atgggcttga tgtcgaagtt cttcaccagc actcccgcga cgttgggaga
     1741 gaggaatccg ggaagggtgg gcccgaggcg gatgcccttc acgccgagcg agaggagggc
     1801 cagaagaacc gcga
//
LOCUS       DAOTIL010000611         1813 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminobacteriaceae bacterium isolate AMR_MDS_3026
            NODE_76790_length_1813_cov_3.670080, whole genome shotgun sequence.
ACCESSION   DAOTIL010000611 DAOTIL010000000
VERSION     DAOTIL010000611.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868050
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminobacteriaceae bacterium (wastewater metagenome)
  ORGANISM  Aminobacteriaceae bacterium
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae.
REFERENCE   1  (bases 1 to 1813)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1813)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:19:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,224
            CDSs (total)                      :: 3,198
            Genes (coding)                    :: 3,184
            CDSs (with protein)               :: 3,184
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1813
                     /organism="Aminobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_76790_length_1813_cov_3.670080"
                     /isolate="AMR_MDS_3026"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:3034609"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..964
                     /gene="rny"
                     /locus_tag="P5201_11395"
     CDS             <1..964
                     /gene="rny"
                     /locus_tag="P5201_11395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006583157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004521 - endoribonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006402 - mRNA catabolic process [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ribonuclease Y"
                     /protein_id="HRV99191.1"
                     /translation="CAVEHTSESAVSVVHLPSDEMKGRIIGREGRNIRTFETLAGVDL
                     IVDDTPEAVTLSSFDPVRREIARLSLERLVTDGRIHPARIEELIEKATKDVENQIMEA
                     GESALLETGVKNMHPELVRTLGQLKFRFSYGQNALQHSLEVAYVSGMIAAELGIDEEI
                     ARRAGLLHDLGKAVDHQVEGPHALIGADLARRFNESPEVVSAIASHHEDQEAKSIYDV
                     IVASADAVSASRPGARRESLDAYVKRLEKLEELAKTFNGVSKAYAIQAGREIRVVVTP
                     NITDDAIVHKIAYDIARKIEEEMKYPGQIKVTATKETRATEYAK"
     gene            970..1749
                     /locus_tag="P5201_11400"
     CDS             970..1749
                     /locus_tag="P5201_11400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015555772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00282 family metallophosphoesterase"
                     /protein_id="HRV99192.1"
                     /translation="MKILFIGDIMGSPGRKAVAAFLPQLSEREGGFDFVLANCENAAA
                     GKGITQKIVNELFAMGISGMTSGNHIWDKSEGVPVLAAESRIVRPANYPDVCPGQGAM
                     LLENGERRLMVANLQGRVFMPPLDCPFRKADELVAAAGDVPLVIDFHAEATSEKKALG
                     YYLDGRVAALLGTHTHVQTADEEILPQGTAYITDAGMTGGHRSSIGMKLEGVLPRYLT
                     GMPSKFEVSGDGLAFNGVVITLDDETGRAVGITRINERLSH"
BASE COUNT          442 a          474 c          554 g          343 t
ORIGIN      
        1 ctgtgcagtc gagcacacat cggaatcggc agtgagcgtg gtgcatcttc cctcggacga
       61 gatgaaggga agaattatcg gcagagaggg acggaacatc cgtaccttcg aaaccctggc
      121 gggagtagac cttatcgtcg acgacacacc ggaagccgtt acgctgagca gcttcgaccc
      181 tgtgcgccgc gagatcgcca gactgtcgct ggaacgtctc gtgaccgacg gaagaataca
      241 tccggccagg atcgaggagc ttatcgagaa ggcaaccaag gatgtggaga atcagattat
      301 ggaggccggc gagagcgctc tgctggagac cggcgtcaaa aatatgcacc cggagctggt
      361 acgaactctc ggtcagctca aattccggtt cagctacggg caaaatgcgc ttcagcacag
      421 ccttgaagtg gcgtacgtgt ccggaatgat cgccgctgaa ctgggcattg acgaggaaat
      481 cgcccgtcgg gctggtctgc tccatgatct gggcaaggcc gtggatcatc aggtagaagg
      541 tccccacgct ctgatcggcg cggatcttgc gcgacggttc aacgaaagtc ccgaggtcgt
      601 aagcgctatc gcttcgcatc acgaagatca ggaagcgaaa agcatctacg atgtcattgt
      661 cgcctccgcg gacgcagtaa gcgcatcgcg tcccggggcg cgccgcgaaa gcctcgacgc
      721 ctatgtcaaa cgtcttgaaa aactcgagga actcgcaaag acgttcaacg gagtcagcaa
      781 ggcgtacgca atccaggccg ggcgcgaaat tcgcgtggtg gtgaccccga atattaccga
      841 cgatgcgatc gtgcataaaa ttgcgtacga tattgcccga aagattgaag aagagatgaa
      901 gtatcccggc cagataaaag ttacagcgac gaaggagacg cgcgccacgg agtacgcgaa
      961 gtaaccgcta tgaagattct ttttatcggc gatatcatgg gaagcccggg aagaaaggct
     1021 gtcgccgcct ttcttccgca gctgtcggag cgcgagggag gatttgattt tgtcctcgcc
     1081 aactgcgaaa atgccgccgc cgggaaaggc attacccaga aaattgtcaa tgagctgttc
     1141 gccatgggga tctccggaat gacttcaggc aatcatatct gggacaagag cgaaggcgtt
     1201 ccggtgctgg ctgccgaatc gaggatcgtt cgcccggcga actacccgga cgtatgtccg
     1261 ggccaggggg cgatgctgct tgagaacgga gaacgacgcc tgatggtggc gaacctgcag
     1321 ggaagggttt ttatgcctcc cctcgattgc ccctttcgca aagcggatga acttgtggcc
     1381 gcggcgggcg atgttccgct cgtgattgat ttccacgcgg aggcgacttc tgaaaaaaaa
     1441 gcgctcgggt actatctgga tgggagagtc gcagccctct tgggaacgca tacgcacgtc
     1501 cagaccgccg acgaggagat acttccgcag ggaactgcat acatcacgga cgccggtatg
     1561 accggcgggc acagaagctc catcggcatg aagctcgaag gcgttcttcc gaggtacctg
     1621 acggggatgc cttccaagtt tgaagtttcc ggggacggcc ttgcattcaa cggagttgtt
     1681 attacgctcg atgatgaaac cggaagagcg gtcggcataa cgagaatcaa cgaaagactg
     1741 tctcactgaa aacgactgaa ggacaaagac gaaagaggga ggcgcatatg cgcctccctc
     1801 tttcgtcttt gtc
//
LOCUS       DAOTIL010000612         1809 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminobacteriaceae bacterium isolate AMR_MDS_3026
            NODE_77035_length_1809_cov_4.202395, whole genome shotgun sequence.
ACCESSION   DAOTIL010000612 DAOTIL010000000
VERSION     DAOTIL010000612.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868050
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminobacteriaceae bacterium (wastewater metagenome)
  ORGANISM  Aminobacteriaceae bacterium
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae.
REFERENCE   1  (bases 1 to 1809)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1809)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:19:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,224
            CDSs (total)                      :: 3,198
            Genes (coding)                    :: 3,184
            CDSs (with protein)               :: 3,184
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1809
                     /organism="Aminobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_77035_length_1809_cov_4.202395"
                     /isolate="AMR_MDS_3026"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:3034609"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..536
                     /locus_tag="P5201_11405"
     CDS             <1..536
                     /locus_tag="P5201_11405"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRV99193.1"
                     /translation="LLIDVLSQDLGILAADGTPVTLLEKGLHLPVRAERHFYSVGNGP
                     FTMSVFQGEGSSRRIIASVQAPEARKDEEITLSFSVDSDGLLRIDIIRSDGRVSSIAP
                     LELGEGVPPSPTETTEEAKGLERRFARLSVSLSPAQQARGAALLRTMKTLGDGDYSSE
                     AFDSLERMISEMERVVR"
     gene            539..1531
                     /locus_tag="P5201_11410"
     CDS             539..1531
                     /locus_tag="P5201_11410"
                     /inference="COORDINATES: protein motif:HMM:NF012451.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaJ domain-containing protein"
                     /protein_id="HRV99194.1"
                     /translation="MSVVIELTDCFEILGVQPGAHLREIRSAFRRLALSCHPDIAGPQ
                     KAKQFETIAAAYAQLKSATPAQISESLKKKKSHGDAGKGSPFSWKKTPPRRPKNERRE
                     EAERESSKRVQDLLLEKALVEAELSMARILEKATRKDEKRTVSSLSKRLLSTHPGVRV
                     LALGALAGRPVEKNVFSSLIEMLRMWSPDDDTVEHLLLLDFSEEQKGDIAAALSARSL
                     LLSEASALALLRWASILPVKMSVVRKLLSHPSPRVLSQALSRWSEKSLPDDLTLIRLL
                     KREEDEVLAPLLRILKDRKVPVWAYGRVKMLAENHVSSAVRVWAQSIVRAQNLV"
     gene            1608..>1809
                     /locus_tag="P5201_11415"
     CDS             1608..>1809
                     /locus_tag="P5201_11415"
                     /inference="COORDINATES: protein motif:HMM:NF013401.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HIT domain-containing protein"
                     /protein_id="HRV99195.1"
                     /translation="MEKVFEDDAVFVIRDISPQAPHHLLVIPKEHVESAADVQNPEVW
                     SKVLDCAVRIASSFGLDNEGYRL"
BASE COUNT          393 a          463 c          527 g          426 t
ORIGIN      
        1 gtcttctcat cgacgtcctc tcgcaggact tgggaatatt ggctgccgac ggaactcctg
       61 tgacgctcct ggaaaaaggt ttgcaccttc ctgttcgtgc ggaaaggcat ttctacagtg
      121 tcggcaatgg tccgttcact atgagcgttt tccagggcga aggctcctca cgaaggatca
      181 ttgcttccgt acaggctccc gaggcgagaa aagacgaaga aataacgctc tccttctcgg
      241 tagactcgga cgggctctta agaattgata taatacgttc cgacggaaga gtttcctcta
      301 tcgcgcctct tgagcttgga gagggcgtcc ctccttcacc tacggagacg accgaggagg
      361 cgaaggggct cgaaaggcgg tttgcgcgtc tttccgtatc gctttccccg gcgcagcagg
      421 cgcgaggagc ggcgttgctg cgcacgatga aaacccttgg agacggagat tattcctccg
      481 aggcttttga ctcgctggaa cggatgattt cggaaatgga gagggttgtg cgatagaaat
      541 gagcgttgtt atcgagttga cggattgttt cgagatacta ggggtccagc ccggagcgca
      601 tctgagggaa atccgctcgg cgttccgcag gcttgcgctc tcctgccatc cggatattgc
      661 cggaccgcaa aaggcgaaac agttcgaaac tatcgccgca gcctacgcgc agttgaagtc
      721 cgcaactccc gcgcaaattt ccgagtcgtt gaaaaaaaag aagtcgcatg gggacgccgg
      781 aaaaggttcg cccttttcgt ggaaaaaaac tcctccccgg cggccgaaga acgaacgtcg
      841 tgaagaggcg gaacgagaga gctcgaaacg ggttcaggac ctgcttcttg aaaaggcgct
      901 ggtcgaagcg gagctctcca tggccaggat tcttgagaaa gcgacgcgga aggatgaaaa
      961 aaggacagtt tcatcgctgt cgaaacgtct cctgagcact caccccggag tgcgtgtgct
     1021 tgcgcttgga gctcttgccg gacgccctgt ggagaaaaac gttttctcct ccttgataga
     1081 gatgcttcga atgtggtctc ccgacgacga tacagtggag caccttcttc tcctggattt
     1141 ctccgaggag cagaaggggg atattgccgc cgccttgagc gcacgaagtc tcctgctctc
     1201 ggaggcttcc gcgctcgctc tgctgcggtg ggcgtctatt ctgcccgtga agatgtccgt
     1261 tgtacgaaaa cttttgtccc acccttctcc gcgggttctt tcgcaagcgc tctcccgatg
     1321 gtcggagaag tcgcttcccg acgatctgac gctcattcgg ttgctcaaga gagaagaaga
     1381 cgaagtcttg gcgccgctgc tgcggattct caaagacagg aaagttcccg tatgggcgta
     1441 tggccgggtg aagatgctcg ctgaaaatca tgtgtcatct gccgtacgag tttgggcgca
     1501 gtctattgtg cgcgctcaaa atctggtata gaatactgtc acgttgtgag gtgggatttt
     1561 tggacggaaa atgtcttttc tgcgaaatgg cttcgggagc agtgcatgtg gaaaaagtct
     1621 tcgaggatga tgctgtattt gtgattcgcg atatctcccc tcaggctccg catcatcttc
     1681 tcgtaatacc gaaagagcat gttgaatcgg ctgccgacgt tcaaaacccc gaagtgtggt
     1741 cgaaagtcct tgattgcgcc gtccgcatcg cttcatcctt tggtcttgac aacgagggat
     1801 acaggcttg
//
LOCUS       DAOTIL010000613         1809 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminobacteriaceae bacterium isolate AMR_MDS_3026
            NODE_77036_length_1809_cov_4.062714, whole genome shotgun sequence.
ACCESSION   DAOTIL010000613 DAOTIL010000000
VERSION     DAOTIL010000613.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868050
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminobacteriaceae bacterium (wastewater metagenome)
  ORGANISM  Aminobacteriaceae bacterium
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae.
REFERENCE   1  (bases 1 to 1809)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1809)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:19:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,224
            CDSs (total)                      :: 3,198
            Genes (coding)                    :: 3,184
            CDSs (with protein)               :: 3,184
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1809
                     /organism="Aminobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_77036_length_1809_cov_4.062714"
                     /isolate="AMR_MDS_3026"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:3034609"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1809)
                     /locus_tag="P5201_11420"
     CDS             complement(<1..>1809)
                     /locus_tag="P5201_11420"
                     /inference="COORDINATES: protein motif:HMM:NF013069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HRV99196.1"
                     /translation="RAFDILEDRVQRQLSQVVDVADVSLDGGLTREIHVDLDPVALGS
                     LGLSIRQVTNLLKSGNYSSPSGRISLGDQESILRVVGESTTVRELEELQIPLAKGVSV
                     RLADIATVTDATAEVRRLARYNGRDSFIISLTAVPDGNVVQASRAARAMLDEILPTLP
                     PGFSMAIPFDDGEFIADSVWNVFRDMITGTLLTGLILFLFLRRLSVTLLVGIVMPTAI
                     IATFMPMVLARYTLNMMTTLGLAISTGILVNNSILVIENIIRYRELGYNPLDAAERGT
                     KEIAIAVLSTTATNLGVFIPMALSGGRMGQMFNPFAMTVVFSTLFSLWVALTFTPMLA
                     ARIGRSAGPSALNRFLTGWWQWLYQGFEELHHVLVRGAVRFPLSTILLFCALSAGSLM
                     LAGRIGFQFFPSSDEGQITISMETSASASLSMTDRFVRQMEQHAISLPYVSGVNAVIG
                     GGGSRSGLNRATMRIYLEDTPDRPSSFAFAAPLRPLLASLPDMKASVTAGGGRGGVRG
                     KPIQVVVRGEEIGALNDLAVKVMEVLRGINGVVDVDLDWRTGRPEIQMIPDRLRMARL
                     NFNSEDLSDMLRGYVTGLKAGVFREGGKEYDILVKL"
BASE COUNT          453 a          527 c          496 g          333 t
ORIGIN      
        1 gaagcttgac gaggatatcg tactccttgc caccctctcg gaagaccccg gccttcagtc
       61 ctgtgacgta tccccggagc atatcggaga gatcctccga attgaaattc agcctcgcca
      121 tccggagtct gtcgggaatc atctgaatct ccgggcggcc tgtccgccag tcgagatcca
      181 catcaaccac accgttaatg ccgcggagaa cctccatgac cttcacggca aggtcgttca
      241 gagcgcctat ttcctctccc cggacaacaa cttgtatagg ctttccgcga acgccgccgc
      301 gtccacctcc ggcagtgacg ctcgccttca tatccgggag agatgccaaa agaggacgga
      361 gaggagccgc aaaagcaaag ctgctaggcc tgtccggcgt atcctcaaga taaatgcgca
      421 ttgtggcacg gttcagtcct gatctcgaac cgccgccgcc aataacagcg ttgacaccgg
      481 agacataagg aagtgaaatg gcgtgttgtt ccatctgccg gacaaaacga tccgtcatcg
      541 agagagaagc ggaggccgag gtctccatgc tgatggtaat ctgcccctcg tcggaggaag
      601 gaaaaaattg gaacccgata cgccctgcga gcatgagaga accggcggaa agagcgcaaa
      661 aaagcaggat ggtggagagc ggaaaacgta cggcgcctcg cacaagtacg tggtgaagct
      721 cttcaaaacc ttgatagagc cactgccacc atcccgtcaa aaaacggttc aaagccgacg
      781 gcccggcaga ccttccgatg cgggccgcca gcatcggcgt gaacgtcaag gcaacccaga
      841 gcgagaagag cgtcgaaaag accaccgtca tggcgaacgg gttaaacatc tgccccatgc
      901 ggccgccgga aagcgccatg gggatgaata cccccagatt tgtcgctgtg gtggaaagca
      961 ccgcgatcgc gatctccttc gtaccgcgct ccgcggcgtc cagcgggttg tatcccaatt
     1021 cccggtacct gatgatgttt tcgataacca ggatagagtt gttcacaaga atgccagtcg
     1081 agatggcgag cccgagggtc gtcatcatgt tgagcgtata gcgcgcaagc accatcggca
     1141 tgaaggtcgc aattatggca gtcggcatga caattcccac aagcagcgtc accgaaaggc
     1201 ggcgcaaaaa gagaaagaga atcaaccccg tcagtagcgt accggtaatc atatcccgga
     1261 agacgttcca caccgagtcg gcgatgaatt ctccgtcgtc gaacggaata gccattgaga
     1321 aaccaggtgg gagcgtcggg aggatctcgt cgagcatcgc ccgagcggcg cggctcgcct
     1381 gtacaacatt gccatcggga acagcagtaa gcgagatgat gaacgaatcc cgtccgttgt
     1441 atcgtgcaag acggcggacc tccgcggtgg catcagtgac cgtcgcaatg tccgcaagac
     1501 gtacggaaac accttttgct aaaggaatct gcagctcttc caactctcgc acggttgtcg
     1561 attcaccgac tacgcgtaga attgactcct gatcaccaag cgaaatccgc cccgacggag
     1621 agctgtagtt accgcttttc agaaggttcg tcacctggcg gatactcaga ccgagactgc
     1681 cgagcgccac agggtcaagg tcgacatgaa tttcccttgt cagtcctccg tccagcgaca
     1741 catcggcaac gtcgacaact tgtgaaagct gtcgttgcac cctgtcttca aggatatcga
     1801 acgctctct
//
LOCUS       DAOTIL010000614         1809 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminobacteriaceae bacterium isolate AMR_MDS_3026
            NODE_77055_length_1809_cov_2.862600, whole genome shotgun sequence.
ACCESSION   DAOTIL010000614 DAOTIL010000000
VERSION     DAOTIL010000614.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868050
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminobacteriaceae bacterium (wastewater metagenome)
  ORGANISM  Aminobacteriaceae bacterium
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae.
REFERENCE   1  (bases 1 to 1809)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1809)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:19:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,224
            CDSs (total)                      :: 3,198
            Genes (coding)                    :: 3,184
            CDSs (with protein)               :: 3,184
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1809
                     /organism="Aminobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_77055_length_1809_cov_2.862600"
                     /isolate="AMR_MDS_3026"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:3034609"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..366)
                     /locus_tag="P5201_11425"
     CDS             complement(<1..366)
                     /locus_tag="P5201_11425"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRV99197.1"
                     /translation="MASFIHNLLRRSGVQPLRLSCSLLRLLSLLFALPSAASAVDLII
                     EMDAVVSVTGNPFPLSEAARLSGDPALVQKTGALLFDIPPDGLLTRDTLLNTLVAKGI
                     GGIRLKLVMPPEMRIVLDES"
     gene            complement(371..1159)
                     /gene="flgG"
                     /locus_tag="P5201_11430"
     CDS             complement(371..1159)
                     /gene="flgG"
                     /locus_tag="P5201_11430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006583268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009426 - bacterial-type flagellum basal
                     body, distal rod [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal-body rod protein FlgG"
                     /protein_id="HRV99198.1"
                     /translation="MLRALWTSASGMIAQQTNLDVTTNNLANVNTSGFKKKRADFADL
                     LYQINREPGAPVEPASMVPTGVQVGLGVRVVGTPRIMTQGNLQVTDRPLDITIEGDGF
                     FQVLQPNGEVAYTRAGGWNLDGEGQVVNTDGLLIEPAILVPEDATEIMISPEGLVSVK
                     IDGQPEPEEIGQIELARFVNSGGLRAIGKNLFLETAASGAPIVGNPGDEGLGQVHQGI
                     LEMSNVQVVDEMVNLIIAQRAYEANSKGVQTADELLRIANGLKR"
     gene            complement(1176..>1809)
                     /locus_tag="P5201_11435"
     CDS             complement(1176..>1809)
                     /locus_tag="P5201_11435"
                     /inference="COORDINATES: protein motif:HMM:NF018168.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="flagellar hook-basal body protein"
                     /protein_id="HRV99199.1"
                     /translation="EIKLIAPPFQLGLKGKFLIGDLSLANVISETYMTTESGAIQVTD
                     NPFDAAIVGEGYFAVQDGAGNIYYTRSGHFQKNGEGQLVTEDGMLVLGDGGPIEVGDA
                     SRFAITENGNVIADGAVVDMFQVVTFENPTYLRQVGRTALAVTPQSGAPVPVEAIRLE
                     PGVLEMSNVNVVEEMVRMVEAHRAYESASKVLMTHDELTGKLISSYGRTS"
BASE COUNT          342 a          515 c          564 g          388 t
ORIGIN      
        1 agattcatca aggacaatac gcatctcggg aggcatgacg agcttgagtc tgatgccgcc
       61 gatgcccttt gcaacaaggg tgttgagtaa ggtatcgcgc gtcagaaggc cgtcgggagg
      121 gatatcgaaa aggagcgctc cagtcttctg caccagggca ggatcgccgg agaggcgggc
      181 cgcctccgaa agaggaaagg ggttgcccgt gacggagacg acggcgtcca tctctatgat
      241 gagatcgacg gcggacgcgg ccgaaggaag agcgaaaaga agcgagagaa gacgaaggag
      301 ggagcaggaa aggcggaggg gctgcacacc gctccgccgt aggagattgt gaatgaaaga
      361 cgccacgggc ctacctcttg aggccgttgg ctattcggag cagttcgtcc gctgtctgca
      421 ctcccttgga gtttgcttcg tacgctctct gtgcgatgat caggttgacc atctcgtcga
      481 ccacctggac gttggacatt tcgaggatgc cctgatgcac ctgtccgagc ccttcatcgc
      541 ccgggttgcc gacgatgggc gcgccgctgg ccgctgtttc gaggaagagg ttctttccga
      601 tggcgcgaag tcctcctgaa ttgacaaaac gtgcaagttc tatctggccg atctcctccg
      661 gctccggctg tccgtctatt ttgaccgaga ccagcccttc cgggctgatc atgatctcgg
      721 tagcgtcctc gggaacaagg atcgcgggct cgatgagcag tccgtccgtg ttgacgacct
      781 gtccttcccc gtcgagattc cagccgcctg cgcgggtata ggccacttcg ccgttaggct
      841 gaagtacctg aaagaaaccg tccccctcta tggtgatgtc gagaggacgg tccgtcacct
      901 ggaggttccc ctgcgtcatg atgcggggcg ttccgacgac ccggacgccg agtccaacct
      961 ggacgccggt cggcaccatc gacgcgggtt cgacgggtgc gccgggttct ctgttgatct
     1021 ggtagagcag atccgcgaag tcggcccgct tcttcttgaa gccggaagta ttcacgttcg
     1081 cgaggttgtt ggtagtgacg tcgagattgg tctgctgcgc gatcatcccg cttgcgcttg
     1141 tccataaggc tctgagcatt tctttctacc tccggttaac tcgtcctgcc gtaggacgag
     1201 atcagttttc cagtgagttc gtcgtgcgtc atgagcactt tggaggccga ctcgtacgcg
     1261 cggtgcgcct cgaccatccg gaccatctcc tcgaccacgt tcacgttgga catctccaac
     1321 acccccggtt caaggcgaat tgcttcgacc ggaacgggag cgccggactg gggagtgact
     1381 gcaagcgcag tccgcccaac ctgtctaaga tacgtggggt tttcgaaggt cacaacctgg
     1441 aacatgtcga ccacggcacc gtcggcgatt acattgccgt tttcggtaat ggcgaaacgg
     1501 gacgcgtcgc ccacttcgat cggtcccccg tctccgagga caagcatccc gtcctccgtg
     1561 acaagctgcc cttccccgtt cttttgaaag tgcccggatc gggtgtagta gatgtttccg
     1621 gcgccgtcct gaacagcgaa gtacccttcg ccgacgatcg ccgcgtcgaa ggggttgtcg
     1681 gtgacttgga ttgcgccgga ctcggtggtc atgtaggttt ccgagataac gttggcgaga
     1741 ctgaggtcgc cgatgagaaa cttccctttg agtccgagct ggaaaggcgg ggcgatgagt
     1801 ttgatctcg
//
LOCUS       DAOTIL010000615         1808 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminobacteriaceae bacterium isolate AMR_MDS_3026
            NODE_77093_length_1808_cov_5.090131, whole genome shotgun sequence.
ACCESSION   DAOTIL010000615 DAOTIL010000000
VERSION     DAOTIL010000615.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868050
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminobacteriaceae bacterium (wastewater metagenome)
  ORGANISM  Aminobacteriaceae bacterium
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae.
REFERENCE   1  (bases 1 to 1808)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1808)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:19:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,224
            CDSs (total)                      :: 3,198
            Genes (coding)                    :: 3,184
            CDSs (with protein)               :: 3,184
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1808
                     /organism="Aminobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_77093_length_1808_cov_5.090131"
                     /isolate="AMR_MDS_3026"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:3034609"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..198
                     /locus_tag="P5201_11440"
     CDS             <1..198
                     /locus_tag="P5201_11440"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRV99200.1"
                     /translation="QDDLRESEHLSAPSTDENCTELLPDLTEQTHRLNFAIHTYLYLH
                     SQTPWLRPAMSSIEFLFLKVL"
     gene            complement(249..>1808)
                     /locus_tag="P5201_11445"
     CDS             complement(249..>1808)
                     /locus_tag="P5201_11445"
                     /inference="COORDINATES: protein motif:HMM:NF012376.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein phosphatase 2C domain-containing
                     protein"
                     /protein_id="HRV99201.1"
                     /translation="DVTSARLGKRLFPEGEGLSKEDFLVVLGDFGLFWHTPRTPEERR
                     CLRSLADRPWTTLFIDGNHENFDLLDALPTEERWGAPVGVAAPGVYHLRRGFVYDVAG
                     LSCFVFGGGRSVDKSVRTPGIDWWERENPGPEERTLGLENLERHGWKVDLVWTHVAPT
                     RACDRLLSDHYAFAHTGRGTAHDPLSDYLDDVAERLSFKLWSFAHYHVSARPFFAGSS
                     GLFTAEYETFREIPIRSGTLPEPKEESAANAEGMDMQLFFFTNKGNVRDANQDALLAG
                     ERLVAYEPGKPSHCMEQVEAVRSTGNRVLLAVIDGMGGYAGGELASRIVAESLLDRLP
                     EVISAASAEAAKEYVVRALGTAAELMNELSAEYESLESMGATLAGLVLGKERALLFNV
                     GDCRVYRLRGGVLERVSRDHSEVQRLVDAGVIKEEEMRFHPRKSIVTSAIQAGVGLAS
                     TPLFFKEFVPREGDAFFLCSDGVWEALSREEIEECLLMPDGKAGCEELRERLLDTECR
                     DNVSFVCMRMN"
BASE COUNT          340 a          585 c          501 g          382 t
ORIGIN      
        1 caagacgacc ttcgtgaatc cgagcacctt tctgctccgt caaccgatga aaactgcacc
       61 gaacttcttc cggatctgac cgaacaaaca caccgtctga atttcgctat ccatacctat
      121 ttgtatttgc attcccaaac gccgtggctt aggccggcca tgtcttccat tgagtttctc
      181 ttcctcaaag tactttaggc tgttcacatc aggctctttg tgccgcaaaa tcctttcgct
      241 tttttttatt agttcatccg catacagacg aagctgacgt tgtcccggca ctccgtatcc
      301 agcagtcttt cccgtagttc ctcgcacccc gcctttccat cgggcatgag caggcactcc
      361 tctatctctt cccgcgacaa cgcctcccag acgccgtcgc tgcacagaaa gaaagcgtcc
      421 ccctcacgcg gaacgaactc cttgaaaaaa agaggggtgg aggcgagacc gacaccggcc
      481 tggatggccg aggtcacgat gctcttccgg gggtggaacc gcatctcttc ctctttgatt
      541 actcctgcgt ccacaagtct ctgtacctcg gagtggtcgc ggctcacccg ttcgagaacg
      601 cctccccgaa ggcgatagac gcggcagtcg cccacgttga agagaagggc gcgctccttt
      661 ccgagaacca gtccggcaag cgtggctccc atcgactcga gagactcata ctccgccgag
      721 agttcgttca tcagctccgc agccgtcccg agcgcccgaa cgacgtactc tttggcagct
      781 tccgcagatg ctgctgagat aacctccgga aggcggtcga gcagggattc cgcgacgatc
      841 cgcgaggcga gttctccccc cgcgtatccg cccatcccgt cgatcaccgc gagaagaacg
      901 cggtttccgg tggaacggac cgcctcgacc tgttccatgc agtgcgatgg cttccctggt
      961 tcgtacgcta ccagccgttc tccggccaga agagcgtcct ggttggcgtc gcgcacattt
     1021 cccttgttgg tgaagaaaaa cagctgcata tccatccctt cagcgttcgc ggcgctttct
     1081 tccttcggtt cggggagggt accggaacgg atcgggattt cacggaaggt ctcgtactcg
     1141 gcggtgaaaa ggccgcttga accggcgaag aagggacgcg ccgagacgtg gtagtgcgcg
     1201 aagctccaga gtttgaagga gagcctttcg gcgacatcgt cgaggtagtc ggataacgga
     1261 tcatgggcgg ttccccttcc ggtgtgcgca aaggcgtagt ggtcgctcag cagtctgtcg
     1321 cacgccctcg tcggcgcgac gtgcgtccag acgaggtcca ccttccatcc gtgccgttcg
     1381 agattctcca atccgagcgt tcgctcctcc ggaccggggt tctctctttc ccaccagtcg
     1441 attcccggag tgcggacgct cttgtcgacg cttctgcctc ctccgaagac gaagcaggag
     1501 aggcctgcca cgtcgtagac gaaccccctt ctgagatggt agacgccggg agcggcgacg
     1561 ccgaccggag cgccccatcg ctcctccgtc gggagagcgt cgagcaggtc gaagttctcg
     1621 tggttgccgt cgatgaagag cgtcgtccac ggtctgtcgg caaggcttct caggcagcgc
     1681 cgctcctcgg gtgtccgagg ggtgtgccag aagagtccga agtcccccag cacgacgaga
     1741 aaatcctctt tgctcagccc ctccccctcg ggaaagagcc gtttgccgag cctcgccgaa
     1801 gtcacgtc
//
LOCUS       DAOTIL010000616         1808 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminobacteriaceae bacterium isolate AMR_MDS_3026
            NODE_77123_length_1808_cov_2.774672, whole genome shotgun sequence.
ACCESSION   DAOTIL010000616 DAOTIL010000000
VERSION     DAOTIL010000616.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868050
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminobacteriaceae bacterium (wastewater metagenome)
  ORGANISM  Aminobacteriaceae bacterium
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae.
REFERENCE   1  (bases 1 to 1808)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1808)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:19:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,224
            CDSs (total)                      :: 3,198
            Genes (coding)                    :: 3,184
            CDSs (with protein)               :: 3,184
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1808
                     /organism="Aminobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_77123_length_1808_cov_2.774672"
                     /isolate="AMR_MDS_3026"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:3034609"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..359
                     /locus_tag="P5201_11450"
     CDS             <1..359
                     /locus_tag="P5201_11450"
                     /inference="COORDINATES: protein motif:HMM:NF015693.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="flagellar basal body-associated FliL family
                     protein"
                     /protein_id="HRV99202.1"
                     /translation="LNPEGSTSVTIEEPGPVYFVGDFIANLSGAGNHVANFKLSLEVS
                     SDRAMEMVSSASWVARIRNEVILLVKDRVFDELTTAEGIMQLAEDIKRTINAMMPSVR
                     DKAPVVRVLFEGFVLQ"
     gene            395..1426
                     /gene="fliM"
                     /locus_tag="P5201_11455"
     CDS             395..1426
                     /gene="fliM"
                     /locus_tag="P5201_11455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012870065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009288 - bacterial-type flagellum
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor switch protein FliM"
                     /protein_id="HRV99203.1"
                     /translation="MTPDVMSQSEIDSLLEALSTGSVDVDSITRDSDEKKIKSYDFRR
                     PDKFSKDQLRAIQMIHESFTRQLTSTMSTMVRSMVSAEIASVDQLAYDEFIRSLVQPT
                     VIGILEMYPFSGNALIEINPNLVFAIIDRMLGGRGEFTGKIRELTDIERTVVERLLMR
                     MLELLEEAWSTVVDVRFRYENLESNPFFVQICPGTDMVLLVILKLKVSEVEGMVSICI
                     PYFLMEPIMDKLSSQQWFASTGRKTDERSGEYIQRHLSKVRIPVALELGHTVLSVSDV
                     LQLSVGDVVKLDEVLGDPLDVRIGNMVKFKAVPGTSNGNYAAEISEIIPMEDEVSGLP
                     EKEGGAGDD"
     gene            1419..>1808
                     /locus_tag="P5201_11460"
     CDS             1419..>1808
                     /locus_tag="P5201_11460"
                     /inference="COORDINATES: protein motif:HMM:NF013175.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRV99204.1"
                     /translation="MIDELLSQDEINALLSGGGFGGGAGNDGSSGDMTAEQQHVLNDI
                     ANLCERVGADVNMVRQGIGADTRIGRKFLYAGCGYGGSCFPKDVKALIKTADTNGYSM
                     EVLKAVERVNENQKSVLFEKLVKAYQGD"
BASE COUNT          425 a          404 c          573 g          406 t
ORIGIN      
        1 ggttgaaccc ggaaggttca acgtccgtga cgattgaaga accggggccg gtctacttcg
       61 tcggcgattt catcgcgaat ctctcgggcg ccgggaatca tgtggcgaac tttaagcttt
      121 cacttgaagt aagcagcgac cgcgcgatgg agatggtgtc ctcggcaagc tgggttgcgc
      181 ggatacgcaa cgaagtgatt ctcctcgtta aggatagagt gttcgacgag ttgacgacag
      241 ccgaggggat aatgcagctc gcggaggaca tcaagcgtac aataaacgcg atgatgccct
      301 ctgttcgtga caaggcgcct gtggtcaggg tccttttcga gggatttgtc cttcagtagc
      361 gctacgatga gaatgcggga aggaggcgga aatcgtgacg cctgacgtga tgtcgcaaag
      421 cgagattgac tctttattag aggcgctctc cacgggaagc gttgacgttg attccataac
      481 gcgcgattcc gatgaaaaaa agatcaagag ttacgacttc cgccgccccg acaagttcag
      541 caaggaccag ttgcgggcga tccagatgat ccacgagtcg ttcacccggc agctgacctc
      601 gactatgtcg acgatggtgc ggtcgatggt ctccgctgaa attgcttcgg tggaccagct
      661 cgcttacgac gagtttatcc gctcccttgt gcagcctacg gtgatcggta ttcttgagat
      721 gtacccgttc agcgggaacg cgctcattga gatcaatccc aatcttgtgt tcgcgatcat
      781 cgacaggatg ctcggcggcc gcggagagtt taccggcaag atcagggagc tgaccgatat
      841 cgagcggacg gttgtggagc gtcttcttat gaggatgctt gaactgctag aggaagcgtg
      901 gagcaccgtg gtggatgttc gcttccgtta cgagaatctt gagagcaatc cgtttttcgt
      961 ccagatctgt cccggtacgg acatggtgct gctggtgatt ctgaagctga aagtgagcga
     1021 agtcgaggga atggtgagca tctgcattcc gtatttcctg atggagccga tcatggacaa
     1081 actgagctcg cagcagtggt ttgcctcgac gggaagaaaa accgacgaga ggtccgggga
     1141 gtatatacag agacatctca gcaaggtcag gattccggta gcgttggagc tcgggcacac
     1201 cgttctcagc gtttcggatg ttctgcagct ttcggtgggc gatgtggtga agctggacga
     1261 agtgctcggc gatcctctgg atgttcgtat cggtaatatg gtgaaattca aggccgttcc
     1321 gggaacgtcg aacggcaatt atgcggcgga gatcagcgag atcatcccta tggaggatga
     1381 agtgagcggt cttccggaaa aagaaggagg ggccggcgat gattgatgaa cttctcagcc
     1441 aggacgagat aaacgccctt ttgtccggag gcggtttcgg agggggcgcc gggaacgatg
     1501 ggagttcggg ggacatgacc gcggagcagc agcatgtcct gaacgatatc gcgaacctct
     1561 gtgaacgtgt aggggctgat gtcaatatgg tgcgtcaagg tatcggagca gatacccgca
     1621 tcggaagaaa attcctctat gccggttgcg gatacggtgg ttcatgtttt cccaaggatg
     1681 tgaaggcctt gatcaagaca gccgacacca atggctattc aatggaagtg ctgaaggccg
     1741 tggaaagggt gaatgaaaat cagaagagtg tattatttga gaaactggta aaagcatatc
     1801 agggagat
//
LOCUS       DAOTIL010000617         1807 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminobacteriaceae bacterium isolate AMR_MDS_3026
            NODE_77179_length_1807_cov_3.180936, whole genome shotgun sequence.
ACCESSION   DAOTIL010000617 DAOTIL010000000
VERSION     DAOTIL010000617.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868050
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminobacteriaceae bacterium (wastewater metagenome)
  ORGANISM  Aminobacteriaceae bacterium
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae.
REFERENCE   1  (bases 1 to 1807)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1807)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:19:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,224
            CDSs (total)                      :: 3,198
            Genes (coding)                    :: 3,184
            CDSs (with protein)               :: 3,184
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1807
                     /organism="Aminobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_77179_length_1807_cov_3.180936"
                     /isolate="AMR_MDS_3026"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:3034609"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..270
                     /locus_tag="P5201_11465"
     CDS             <1..270
                     /locus_tag="P5201_11465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013048448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-[acyl-carrier-protein] synthase
                     II"
                     /protein_id="HRV99205.1"
                     /translation="HTDKAYVNSTKSMIGHCLGAAGALETVAALQTIEEGIIHPTINL
                     EHLDPECAINVVGVSPVKAHVSRLLVNSFGFGGHNGVIALQRFEE"
     gene            324..1031
                     /gene="rnc"
                     /locus_tag="P5201_11470"
     CDS             324..1031
                     /gene="rnc"
                     /locus_tag="P5201_11470"
                     /EC_number="3.1.26.3"
                     /inference="COORDINATES: protein motif:HMM:TIGR02191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004525 - ribonuclease III activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease III"
                     /protein_id="HRV99206.1"
                     /translation="MNFEDWYNGKLEKLQESIGYFFDDPTLLRMALLHSSFTHEHGGN
                     DHNERLEFLGDSVLQLSASHLLFISEKQLDEGALSRRRAALVCGNSLRRWAEFIDIPT
                     LLRTGKGMSGGTVRSSIYEDAAEALFGAVFLDGGYKAADAVILRYLDFLMFLSSPEPA
                     DPKTELQILAQERGIGIPSYEILSVTGPSHQPLFHVRVLLGGSPAGEGRGTSKKAAEF
                     NAAREGYTFFTKETATP"
     gene            1028..>1807
                     /locus_tag="P5201_11475"
     CDS             1028..>1807
                     /locus_tag="P5201_11475"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRV99207.1"
                     /translation="MKTKRFLFIFFLGFFLLRASSAFAWEVWTTTPWLALVSRFIGGV
                     FVAVRPIEFWSEDGQVVRKVQNRAIPKDSRIIALDTEEASRLGLNQKEWMSLALLYRT
                     APFDKKRLDYFFADPSSLPFIAQKVFTVLSHFDPANYPYYQRRLSEFQTRLDSTVIVG
                     RRLLSGSAFIYIGGDFSGMLTAAGCSMESRDESLNTLWEQGKELDLLFALLEDALKRR
                     IPVIVDTSSKKLKEALRNNKEILFLEKPALDQDILLFFHDQY"
BASE COUNT          444 a          432 c          430 g          501 t
ORIGIN      
        1 catacagaca aggcttatgt aaactcaaca aaatccatga taggccattg ccttggagca
       61 gccggcgcct tggagaccgt agccgctctt cagactatcg aagagggtat tattcatccg
      121 acaatcaact tggagcatct cgatcctgag tgcgctatca acgttgtggg ggtttccccc
      181 gtgaaagctc atgtttccag gcttctggtg aacagttttg gcttcggcgg ccacaacggt
      241 gttattgcgc ttcagaggtt tgaggagtaa aaagaaagta tagccccgtt ttttcggggc
      301 tgtatttttc ttttcgagga agaatgaatt tcgaagactg gtacaacgga aagcttgaaa
      361 agctgcaaga gagtatcggt tactttttcg acgatcccac tcttttgcga atggcattgc
      421 tgcattcttc gtttacgcac gaacacggcg ggaacgatca caatgaacgc ctggaattcc
      481 tcggcgactc ggtgcttcag ctcagcgctt cccatttgct ttttatctcg gaaaaacagc
      541 ttgatgaagg agctctttca cggaggagag ccgcacttgt ttgcggaaac tctcttcgcc
      601 gatgggcgga atttattgat attccaacgc ttctgaggac agggaaagga atgtctggcg
      661 gcactgtccg cagttcaatc tacgaagatg ccgctgaagc ccttttcgga gcagtttttc
      721 tcgatggagg atataaagcc gctgatgctg ttatactaag atatcttgat tttctaatgt
      781 ttctttcttc tccggaacct gccgacccga aaaccgagct tcagattctc gctcaagaga
      841 gaggcatcgg cattccttcc tacgaaattc tttccgtaac cggtccttct catcaacctc
      901 tatttcatgt gcgagtgctc cttggaggtt ccccggctgg agaaggaaga ggaacatcga
      961 aaaaagcggc agaattcaat gcggcaagag aagggtatac tttcttcacg aaggagacag
     1021 ccacgccatg aaaacgaaac gatttctttt cattttcttt cttggatttt tccttcttcg
     1081 cgcttcctcc gccttcgctt gggaagtatg gacgacgacg ccttggcttg ctctcgtaag
     1141 ccgctttatc ggaggcgttt ttgtagcggt tcgtccgatt gagttttggt ctgaagacgg
     1201 tcaagttgta cgcaaagtac aaaaccgtgc aatacccaaa gacagccgta tcattgcgct
     1261 tgatactgaa gaagcttccc ggcttggatt gaaccaaaaa gagtggatgt cgcttgcact
     1321 tctttaccgt acagctcctt ttgacaaaaa acgtttggat tatttctttg ctgacccgtc
     1381 ttcgcttcca ttcatagcac agaaggtttt cacggtcctc tcacatttcg acccggcaaa
     1441 ctacccctac taccaacgtc ggctttcaga atttcagacg cgtctcgaca gcacggtcat
     1501 cgtgggaaga aggcttttga gcggatcagc ctttatctat attgggggtg atttttccgg
     1561 gatgctgact gcagctggat gttcgatgga atcaagggat gagtcattga atacactttg
     1621 ggagcaagga aaagaactcg accttctttt cgctctcctt gaggatgctc tcaaaaggag
     1681 aattccagta atcgtcgata catcttccaa gaagcttaaa gaagcgctac gcaacaacaa
     1741 agagatactc ttcctggaga aacccgccct tgaccaggat atactgcttt tctttcacga
     1801 tcagtat
//
LOCUS       DAOTIL010000618         1807 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminobacteriaceae bacterium isolate AMR_MDS_3026
            NODE_77182_length_1807_cov_2.945205, whole genome shotgun sequence.
ACCESSION   DAOTIL010000618 DAOTIL010000000
VERSION     DAOTIL010000618.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868050
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminobacteriaceae bacterium (wastewater metagenome)
  ORGANISM  Aminobacteriaceae bacterium
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae.
REFERENCE   1  (bases 1 to 1807)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1807)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:19:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,224
            CDSs (total)                      :: 3,198
            Genes (coding)                    :: 3,184
            CDSs (with protein)               :: 3,184
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1807
                     /organism="Aminobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_77182_length_1807_cov_2.945205"
                     /isolate="AMR_MDS_3026"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:3034609"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1201
                     /locus_tag="P5201_11480"
     CDS             <1..1201
                     /locus_tag="P5201_11480"
                     /inference="COORDINATES: protein motif:HMM:NF014385.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HRV99208.1"
                     /translation="VSIAEGAGEARTLTLEECIRLAEEHHPSLEEARASLNAQKARLD
                     AVKAGNALTGSLSASTGRQTGSDQSYSTSFTVTKLLTDSGKNALERRSQGLAIDTASE
                     SQREAVLTVRKGVKDAYYTLLLNGLKREQAESAVRTYEKHLEKAKGFYEAGIKARFDV
                     TKAEVDLSNARITLVSAEASLATARAALSNAVGIQLGDVEAVSGFLAPRSLPDEPFAV
                     EQAMANRPDIRSALLKSESGKLSVAIAAKGNAATVSLSGSANLSGSEWPLNDTFRATV
                     TLTVPVFDGGLTDARVAEARYGAQGADAALRRVEQTVLYDVRSTLLSVKEAEARIPVA
                     ELLVRQAEENLTLAEGRYEMGVGNVLEVADAVLAFTSAKVSLYQAQHDYSASLAALEK
                     TLGGEFE"
     gene            1198..>1807
                     /locus_tag="P5201_11485"
     CDS             1198..>1807
                     /locus_tag="P5201_11485"
                     /inference="COORDINATES: protein motif:HMM:TIGR01730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HRV99209.1"
                     /translation="MKKIIVLLLLLAGGAGYFFLSDGGATEYTFRTAALESGNIVAKV
                     TATGKLSAVTVVEVGTQVSGTVKEIYADFNDHVKKGQLIALIDPDVLSAKLEESKANL
                     TVARASVARAKANLADSQRNLKRNKELWSRNLIAKSELEAAETTHALNRASLQEAEAR
                     VLQVQAALRQSESNLGYTRILSPEDGVIISRLVNVGQTVAASL"
BASE COUNT          344 a          584 c          617 g          262 t
ORIGIN      
        1 ggtaagcata gcggaggggg ccggcgaggc gcgaacgctc actctcgaag agtgtattcg
       61 ccttgcggag gagcatcatc cgtcgctgga ggaggcgcgg gcgtcgctga acgcgcagaa
      121 ggcaaggctc gacgctgtaa aggccggaaa cgcactcaca ggaagccttt cggcctccac
      181 cggacggcag acgggatcag accagagcta ctcgacctcc ttcacggtga cgaagctcct
      241 caccgactcg gggaagaacg cgctcgaacg gcggagtcag gggctcgcca tcgacactgc
      301 ctccgagtcg cagcgggagg ctgtcctgac cgtccggaag ggcgtgaagg acgcctacta
      361 cacgctcttg ctgaacggac tgaagcgcga gcaggcggag agcgccgtgc gcacctacga
      421 aaagcatctg gagaaggcga agggcttcta cgaggccggg ataaaggcgc ggttcgacgt
      481 caccaaggcc gaagtggacc tgagcaacgc ccggataacc ctcgtctccg cggaggcgtc
      541 gctcgccacg gcgcgtgccg ctctgtcgaa cgcggtcggt atccagctcg gcgacgtcga
      601 agccgtctcc ggctttctcg cgccccgttc gctccccgac gagccctttg cggtcgagca
      661 ggccatggca aacaggccgg acatccgctc cgctcttttg aagagcgagt cgggcaagct
      721 ctcggtcgcc atcgccgcga aggggaacgc cgcgacggtc tccctttcgg gctccgcgaa
      781 cctctccggt tcggagtggc cgctcaacga caccttccgc gccacggtca cgctcaccgt
      841 tcccgtcttc gacggcggac tgacggacgc cagggtcgcg gaggcccgct acggcgcgca
      901 gggggcggac gctgcgctgc ggcgcgtcga acagaccgtc ctctacgacg tccgctcgac
      961 gctcctctcc gtgaaagagg ccgaagcgcg catcccggtg gcggaactgc tggtgcgcca
     1021 ggcggaggag aacctgacgc tcgcggaggg acgctacgag atgggcgtgg ggaacgtcct
     1081 cgaagtggcg gacgccgtgc tcgccttcac cagcgcgaag gtaagcctct atcaggcaca
     1141 gcacgactac agcgcctcgc tggccgcgct ggagaagacc ctcgggggtg agttcgaatg
     1201 aaaaaaatta tcgtactgct gcttctgctg gccgggggcg ccggttactt cttcctctcg
     1261 gacgggggcg ctacggagta cacgttccgc accgccgcgc tggagtcggg gaacatcgtc
     1321 gcgaaggtaa cggccaccgg aaagttgagc gcggtcaccg tggtcgaggt cggcacgcag
     1381 gtctccggga ccgtcaagga gatttacgcc gacttcaacg accacgtgaa gaagggacag
     1441 ctcatcgcgc tcatcgaccc ggacgtcctc agcgcgaagc tggaggagtc gaaggcgaac
     1501 ctcacggtcg ccagggcgtc ggtcgctcgg gcgaaggcca acctcgccga cagccagagg
     1561 aatctgaagc ggaacaagga gctgtggtcg cgcaacctga tcgcgaaaag cgaactggag
     1621 gcggcggaga ccacgcacgc cctcaacagg gcgagccttc aggaggcgga ggcgcgcgtg
     1681 ctccaggtac aggcggccct gcgccagtcg gagtccaacc tggggtacac gcgcatcctc
     1741 tcgccggagg acggcgtcat catctcgcgc ctggtcaacg tcggccagac cgtcgcggcg
     1801 agcctct
//
LOCUS       DAOTIL010000619         1804 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminobacteriaceae bacterium isolate AMR_MDS_3026
            NODE_77366_length_1804_cov_3.303602, whole genome shotgun sequence.
ACCESSION   DAOTIL010000619 DAOTIL010000000
VERSION     DAOTIL010000619.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868050
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminobacteriaceae bacterium (wastewater metagenome)
  ORGANISM  Aminobacteriaceae bacterium
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae.
REFERENCE   1  (bases 1 to 1804)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1804)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:19:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,224
            CDSs (total)                      :: 3,198
            Genes (coding)                    :: 3,184
            CDSs (with protein)               :: 3,184
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1804
                     /organism="Aminobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_77366_length_1804_cov_3.303602"
                     /isolate="AMR_MDS_3026"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:3034609"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            214..777
                     /locus_tag="P5201_11490"
     CDS             214..777
                     /locus_tag="P5201_11490"
                     /inference="COORDINATES: protein motif:HMM:NF019498.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="XRE family transcriptional regulator"
                     /protein_id="HRV99210.1"
                     /translation="MESLEKIVAENLKRLRGERKLSLDRVSALTGVSKSMLGQVERGE
                     SSPTLQTVWKIANGLRIPLSELTDAPKQETESFSKEQVAAILGDNGKFRVYPIFPFDG
                     KNRFEFLSIEMDRGAFSSSEPHIAGTVEYVHVFEGEVVIMTGSEERRLECGDSMKYRA
                     DRPHSYHNPGRERAVLAMVILYPEENV"
     gene            793..>1804
                     /locus_tag="P5201_11495"
     CDS             793..>1804
                     /locus_tag="P5201_11495"
                     /inference="COORDINATES: protein motif:HMM:NF014900.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate formate lyase family protein"
                     /protein_id="HRV99211.1"
                     /translation="MSSSERTESARQEYVHGTPSVSCLRAAIWTESHKETEGEATPVR
                     RAKAFAAACGKLPAIIFPGELIVGVSGEFRRSAILTPEFSWTWVDREMDSFDTRPQDP
                     YRMSPQQREFARSAIWPYWKGKSLEEAFLKRLPEDTARLLVDTGILDNDSKWRQAVGE
                     VTPDYQDVLFPKGYRRIRDEAAAHLAATKPDSLENLERRDFYHSVVIACDGIMRLAER
                     YSEEAMRLAEKEADPVRRGELLEIAGNCARVPAEPPRTFAEACQFVWFVQLGAILSEN
                     PLALNPGRFDQYMYPYYAADVEAGRLTPERALELVECLWFKFSEWVWTISSNTANYFA
                     GYN"
BASE COUNT          375 a          514 c          596 g          319 t
ORIGIN      
        1 gcagaaaaga agacagagag aggtcaaggg tgctttgcga aacacggaaa tccactcctt
       61 ataaaaaaca tgttcagtgc gcaggtaata gtagcacgaa aatgtttatt tgtgttatcg
      121 agttgcaccc ttcgtgggaa atctcttgac agaaacgttc tttttgtgca atataacgca
      181 cataaagaac gctgtgtcag ggaggcaaga gctgtggaaa gtctcgaaaa gatcgtcgcg
      241 gaaaatttga agcgtctgcg gggggagcgg aagctcagcc ttgacagggt gtcggccctt
      301 accggcgtga gcaagagcat gctggggcag gtcgagcggg gagagtcgag cccgacgctc
      361 cagacggtct ggaagatcgc caacggcctg cgcatccccc tcagcgaact caccgacgct
      421 cccaaacagg agacggagtc cttctccaag gagcaggtgg cggcgatact gggagacaac
      481 ggaaagttca gggtgtaccc cattttcccc ttcgacggga agaaccgctt cgaattcctc
      541 tccatagaga tggacagggg ggcattttcc tcctccgaac cgcatatcgc cgggactgtg
      601 gagtacgttc acgtcttcga aggcgaggtc gtcatcatga cggggagcga ggaacggcgg
      661 ttggaatgcg gggactcgat gaagtacaga gcggacaggc cgcactccta tcacaacccg
      721 gggcgggagc gcgcggtgct tgcgatggtc atcctctatc cggaggagaa tgtctgaaag
      781 gggagttgtt tcatgagcag cagtgaacgc accgagagtg cgcggcagga gtacgtgcac
      841 ggcacgccgt ccgtgagctg ccttcgggcg gcgatatgga cggagtccca caaggagacc
      901 gaaggggagg cgacgccggt tcggcgcgcg aaggccttcg ctgcggcgtg cgggaagctc
      961 ccggcgatca tctttcccgg cgagctgatc gtcggcgtct ccggcgagtt tcgccgctcc
     1021 gccatcctca cgccggagtt ctcgtggaca tgggtggacc gcgagatgga ctccttcgac
     1081 acgcgcccgc aggacccgta caggatgtcc ccgcagcagc gggagttcgc ccggtcggcg
     1141 atctggccgt actggaaggg gaagtccctc gaagaggcgt tcctgaagcg tctgccggag
     1201 gatacggctc gtctgctcgt ggacacggga atactggata acgactcgaa gtggcggcag
     1261 gcggtgggcg aggtgacgcc cgactatcag gacgtcctct ttccgaaagg gtaccggcga
     1321 atccgcgacg aggcggcggc gcatctggcc gcgacgaagc ccgattcgct cgagaacctg
     1381 gaaagacgcg acttctacca ctccgtcgtc atcgcctgcg acggaatcat gcgtcttgcg
     1441 gagcgctact ccgaggaggc catgaggctc gccgagaagg aagcggaccc agtacggcgc
     1501 ggcgagctgc tggagatcgc cgggaactgc gcccgcgttc ctgcggaacc tccccggacg
     1561 tttgccgagg cctgccagtt cgtctggttc gtccagctcg gcgcgatcct ctccgaaaac
     1621 ccgctggccc tcaatccggg ccggttcgac cagtacatgt acccgtacta cgcggccgac
     1681 gtcgaggccg gacgcctgac gccggagcgg gcgctcgaac tggtggagtg cctctggttc
     1741 aagttctcgg agtgggtctg gacgatctcc tccaacacgg cgaactactt cgccgggtac
     1801 aacc
//
LOCUS       DAOTIL010000620         1800 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminobacteriaceae bacterium isolate AMR_MDS_3026
            NODE_77607_length_1800_cov_3.282521, whole genome shotgun sequence.
ACCESSION   DAOTIL010000620 DAOTIL010000000
VERSION     DAOTIL010000620.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868050
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminobacteriaceae bacterium (wastewater metagenome)
  ORGANISM  Aminobacteriaceae bacterium
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae.
REFERENCE   1  (bases 1 to 1800)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1800)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:19:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,224
            CDSs (total)                      :: 3,198
            Genes (coding)                    :: 3,184
            CDSs (with protein)               :: 3,184
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1800
                     /organism="Aminobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_77607_length_1800_cov_3.282521"
                     /isolate="AMR_MDS_3026"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:3034609"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(52..945)
                     /gene="aroE"
                     /locus_tag="P5201_11500"
     CDS             complement(52..945)
                     /gene="aroE"
                     /locus_tag="P5201_11500"
                     /EC_number="1.1.1.25"
                     /inference="COORDINATES: protein motif:HMM:TIGR00507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004764 - shikimate 3-dehydrogenase
                     (NADP+) activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate dehydrogenase"
                     /protein_id="HRV99212.1"
                     /translation="MFNAKVNTRFFGLLGNPVGQSAAPFMHNSVYQKMGLDMLYVPLE
                     LEMESLETVVRTMQLFNYAGTGVTMPFKTQVHKYVDGLADSARYTEVVNTIVIGKDGR
                     KIGHNTDGTGFVESLEKQAGLNIPDHRYLLLGSGGAATAIACALAMRGATAMRSLCIR
                     PDYSYAETLYTRVNAHFPGVCEIDEMNDTTIRKGLEEFDVIIHATKVGLYPKTGDILF
                     DPALLSSSHLVCDVVYVPMETKLLVEAEKRGCRTLSGLWMNVYQAAEQMRLWAGIEEP
                     PIDFMYQCGIEYLRREGKLTA"
     gene            complement(968..>1800)
                     /locus_tag="P5201_11505"
     CDS             complement(968..>1800)
                     /locus_tag="P5201_11505"
                     /inference="COORDINATES: protein motif:HMM:NF013429.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase"
                     /protein_id="HRV99213.1"
                     /translation="IMQPQFSLAQLTVLKTSPAEISRIAADCGYDYVSMRQIYMGLPE
                     EPDYDLSKNKKLMAETKAVFADTGIKLLDIELARIFDGMDVKKYEPAMTTAVELGGKH
                     VLSSIWTLDREYAIERFAELCDLAAQYGLTVELEYVPIAGVRTLGGAVDVLRKVNRKN
                     AGLMIDIHHFHRAQDDPAELAKLPEEWFRFAHLCDAPAEIPSEQTEMVRILREARSYV
                     GEGGIDIAPILNAMPVVPYSIELPNLERVAESGYAEHARRCLESAKTFCSGYLKGRSE
                     "
BASE COUNT          351 a          570 c          486 g          393 t
ORIGIN      
        1 cttgcatgac atccccctct agaacgcccc gctcccactc tcgtgggaag ctcaggctgt
       61 cagtttacct tctcttcgaa gatattcaat accgcactgg tacatgaagt caataggagg
      121 ctcttcaata cccgcccaga gacgcatctg ctccgccgcc tggtagacgt tcatccacaa
      181 gccggagagg gtgcggcatc cgcgcttctc cgcctctacg agaagttttg tctccatggg
      241 gacatagacc acgtcgcaca cgagatgtga cgaactcaga agcgccggat cgaagaggat
      301 gtcgccggtc ttcgggtaga gcccgacttt tgtcgcgtgg atgatgacgt cgaactcctc
      361 cagccccttg cgaatcgttg tgtcgttcat ctcgtcgatc tcgcagacgc ccgggaaatg
      421 agcgttcacc cttgtgtaga gcgtctccgc ataggagtag tccggccgga tgcagagcga
      481 acgcatcgcc gtcgcccctc tcatcgcgag agcgcaggcg atcgcggtcg cggcgcctcc
      541 cgagccaagg agaagatacc ggtgatccgg gatgttcagc ccggcctgtt tctcaagcga
      601 ctcgacaaaa cccgttccgt cggtattatg accgatcttt cgtccgtctt tcccgatgac
      661 gatggtgttg accacctcgg tgtaccttgc ggagtcggcc agtccgtcga cgtacttgtg
      721 gacctgtgtc ttgaagggca tcgtaacgcc cgtgcccgca taattgaaga gctgcatggt
      781 gcgaaccact gtttcaagac tctccatctc cagctcaagt ggaacgtaga gcatgtcgag
      841 ccccattttc tggtagacgc tgttatgcat aaagggcgcg gcgctctgtc ccaccgggtt
      901 gcccagaaga ccgaagaatc gcgtgttcac cttcgcgttg aacatattct cgaactcctt
      961 tcccggccta ctctgaacgg cccttgagat acccggagca gaaggttttc gccgactcca
     1021 gacagcggcg tgcatgctca gcgtaccccg actccgccac gcgctccagg ttcggcagct
     1081 cgatcgagta ggggacgacg ggcatcgcgt tcagaatcgg ggcgatgtcg atccccccct
     1141 cccccacata cgatctggcc tccctgagaa tccgtaccat ttccgtctgt tccgagggga
     1201 tttccgcggg cgcgtcgcaa agatgcgcaa agcggaacca ctcctccggc agcttcgcaa
     1261 gctccgcagg gtcgtcctgg gctctgtgga agtgatgaat atcgatcatc agcccggcgt
     1321 tcttccggtt gaccttccgg agcacatcga ctgcgccgcc cagcgtcctg accccggcga
     1381 tgggaacata ctccagctcg acggtcaggc cgtactgcgc ggcgagatcg cacaactccg
     1441 cgaagcgctc tatggcatac tcccggtcga gcgtccagat gctgctcagg acatgcttgc
     1501 cccccaactc gacagccgtc gtcattgcgg gttcgtactt tttgacgtcc atgccgtcga
     1561 atatcctcgc gagttcgatg tcaagcagct tgatacccgt atctgcgaag acggccttcg
     1621 tctccgccat cagtttttta ttcttcgaga gatcgtaatc gggctcctcg gggagcccca
     1681 tatatatctg ccgcatgctc acatagtcgt atccgcaatc cgctgcaatc cgcgagatct
     1741 cggcaggcga cgtcttcagc accgtaagct gcgccaggga aaattgaggc tgcataatcc
//
LOCUS       DAOTIL010000621         1797 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminobacteriaceae bacterium isolate AMR_MDS_3026
            NODE_77768_length_1797_cov_3.741102, whole genome shotgun sequence.
ACCESSION   DAOTIL010000621 DAOTIL010000000
VERSION     DAOTIL010000621.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868050
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminobacteriaceae bacterium (wastewater metagenome)
  ORGANISM  Aminobacteriaceae bacterium
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae.
REFERENCE   1  (bases 1 to 1797)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1797)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:19:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,224
            CDSs (total)                      :: 3,198
            Genes (coding)                    :: 3,184
            CDSs (with protein)               :: 3,184
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1797
                     /organism="Aminobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_77768_length_1797_cov_3.741102"
                     /isolate="AMR_MDS_3026"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:3034609"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..840)
                     /locus_tag="P5201_11510"
     CDS             complement(<1..840)
                     /locus_tag="P5201_11510"
                     /inference="COORDINATES: protein motif:HMM:NF013088.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HRV99214.1"
                     /translation="MTPSSVARLKALVAVIVWGASFPSAKVAVGEVSFTTLVWLRFGS
                     GLLVLFLFLAIRGKIRLLPLREAVTFAALGFLGVFFHNSIQAYALQTVSAGMSGLIIA
                     ANPIAIALLGSLLLSEPLGKAKIAGILLAACGVLVIISRGNLSVFLQREYSFGELVMV
                     LSVFSWGFFSVLSRKALQRTSPELGMTYALTFGWLYSIIPLLANGGVAELPSVSGKAW
                     ANILFLGILCSALAYLFWYDALQVLPASEVGVFLYVNPVVAVIISALFLGESVTSSML
                     FGGV"
     gene            complement(837..>1797)
                     /locus_tag="P5201_11515"
     CDS             complement(837..>1797)
                     /locus_tag="P5201_11515"
                     /inference="COORDINATES: protein motif:HMM:NF012410.2"
                     /note="molecular chaperone; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="molecular chaperone HtpG"
                     /protein_id="HRV99215.1"
                     /translation="SWLRFLRGVVDSEDLPLNISRELLQEDPLLRVIRKSVVRRVLNA
                     LKKMLNESREKYENFWREFGQVIKEGLVSLDGREEENREAILDICLFHTTAGDTMSTL
                     KEYVDALPEGQEHIYYLTGRNLSVLRGSPLLERCADAGYSVLLMADPVDEVAAPTFTE
                     YSGKHFRSLEREDALPKKDDGASSSEVTEGVISFFKEVLGDTVKDVRISERLTTSPAC
                     LVSPDDGGSFAMEQILRSMGQEVPSVKRILEINPHHKVIRSLEQRLSKGEGKESLGEY
                     VFLLYDQCLLAEGGRVEDPALFARRVTAILAESLKDEDGTEER"
BASE COUNT          489 a          514 c          460 g          334 t
ORIGIN      
        1 tactcctccg aaaagcatcg atgaagtcac agactctcca agaaacaagg cagagataat
       61 caccgcaacc acaggattga cgtaaaggaa aacgcctact tctgaagcag ggagcacctg
      121 gagcgcatcg taccagaaca gataggccag cgcagagcaa agaatgccaa gaaaaaggat
      181 attcgcccac gctttgcccg acactgacgg aagctcggcc actccgccat tcgcaagcaa
      241 gggaataatg gaatacaacc acccgaacgt cagcgcgtag gtcataccga gctcgggcga
      301 cgtccgctgc agggcctttc gcgaaagcac cgagaaaaat ccccacgaaa agacgctcaa
      361 caccatgacg agctcaccga aggaatactc cctttgaaga aaaacagaaa gattaccgcg
      421 ggagatgatg acgagcactc cgcacgcagc cagcagaata cctgcgattt tggctttgcc
      481 aagaggttcg gagagaagga gtgaaccaag aagcgctatt gctatcggat ttgctgcgat
      541 aatcaaccct gacatcccgg cggacacagt ctgcaacgca taggcctgta tcgagttatg
      601 gaaaaagacc ccaagaaaac cgagcgcggc gaacgttaca gcctcacgca acggaagaag
      661 gcggattttt ccccgtatcg ccaggaaaag aaacagcacg agcaatcccg agccaaagcg
      721 aagccataca agcgttgtaa aggagacctc ccccacagcg acctttgcgg acgggaagga
      781 ggccccccaa acgatgacag cgacaagcgc tttcaagcga gctacagagg aaggagtcac
      841 ctttcctccg ttccgtcttc gtccttcagg ctttccgcca ggattgcagt caccctgcga
      901 gcgaaaagcg ccggatcctc gactcgaccg ccctctgcga ggagacactg gtcgtagaga
      961 agaaatacgt actcccccaa cgattccttc ccctctcctt tagaaagacg ctgctccagg
     1021 gaacgaatca ctttgtggtg aggatttatc tcaaggatcc ttttcaccga aggaacttcc
     1081 tgccccatcg aacggagaat ctgctccata gcgaaagaac cgccgtcgtc gggagaaaca
     1141 aggcatgcgg gcgaggtcgt aagccgttcg gatattctga catcctttac agtatcccca
     1201 aggacttctt taaaaaaaga aatgacgccc tcggttactt cggaagagga ggcgccgtcg
     1261 tccttcttcg gaagggcatc ctcacgctca agcgaacgga agtgtttgcc ggagtactca
     1321 gtaaatgtag gcgcggcgac ttcgtcgacc gggtcggcca tcagaagcac ggagtacccc
     1381 gcgtcagcgc agcgttccaa aagaggagag cctcgaagaa cggagagatt ccgccctgta
     1441 aggtagtaga tgtgctcctg tccctcgggt agagcatcga catactcctt caacgtactc
     1501 attgtgtctc cggctgtcgt atggaaaaga cagatatcaa gaatagcctc gcggttctct
     1561 tcttcgcgac cgtcaagcga gacgagccct tcttttatta cctgtccgaa ctctctccag
     1621 aagttctcgt acttctctcg gctttcgttc agcatttttt tcagcgcgtt gagcacccgg
     1681 cggacaacgc tttttctgat aacccgcaac agcggatcct cctgcaggag ttcgcgggag
     1741 atgttcagcg gcaagtcctc ggagtcgacc acgccccgca agaagcgaag ccaagag
//
LOCUS       DAOTIL010000622         1795 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminobacteriaceae bacterium isolate AMR_MDS_3026
            NODE_77916_length_1795_cov_3.194253, whole genome shotgun sequence.
ACCESSION   DAOTIL010000622 DAOTIL010000000
VERSION     DAOTIL010000622.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868050
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminobacteriaceae bacterium (wastewater metagenome)
  ORGANISM  Aminobacteriaceae bacterium
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae.
REFERENCE   1  (bases 1 to 1795)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1795)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:19:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,224
            CDSs (total)                      :: 3,198
            Genes (coding)                    :: 3,184
            CDSs (with protein)               :: 3,184
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1795
                     /organism="Aminobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_77916_length_1795_cov_3.194253"
                     /isolate="AMR_MDS_3026"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:3034609"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..347)
                     /locus_tag="P5201_11520"
     CDS             complement(<1..347)
                     /locus_tag="P5201_11520"
                     /inference="COORDINATES: protein motif:HMM:NF024740.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRV99216.1"
                     /translation="MIGDMFDVFFFDLDGVIYIGGETTPGAVSVLDALRSMGKTIRFL
                     TNNPTTRVRIADRLRGHGVAAEMDEIVTSGSATAKYLAGEGIGRAWVIGESGLHKEVE
                     LAGILPAGETDCEA"
     gene            538..1014
                     /locus_tag="P5201_11525"
     CDS             538..1014
                     /locus_tag="P5201_11525"
                     /inference="COORDINATES: protein motif:HMM:NF024273.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinB family protein"
                     /protein_id="HRV99217.1"
                     /translation="MRNAVASIETYYERGMGFLFRLIDECPDELWGKKGGGFFYWQQL
                     YHAFYCVDYFLLPAGSEFPEKPYGSAVAMFRETLDTVPAKDEIREYGAMMKAKADAWI
                     AALDDGMLGDRHEGMSERRKTEVTNAGVLTSLCGHNMYHVGCLDSVLRENGLKGVY"
     gene            complement(1058..>1795)
                     /locus_tag="P5201_11530"
     CDS             complement(1058..>1795)
                     /locus_tag="P5201_11530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013049234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004180 - carboxypeptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HRV99218.1"
                     /translation="DPKPDAMVGLHTGSIWKGFVPGEIGYRPGGIMACMDRFDILIKG
                     KGAHGANPQGSVDPISIAGQIICELQTIISREVDPQEPCVISLGEIHAGSAFNIIPGE
                     CRITGTVRAFNQEMREFLARRIGEVASAVASGMRGSVEFSYGWHGPSPVVNDPVMTEE
                     LRLAAEKIVGPEKVREIPRPSMGGEDVAFFLEKAPGTFFFLPGCNEAKGQTWPHHNSR
                     FNIDEDVLWIGPAVMATMAFDWLKKQA"
BASE COUNT          335 a          542 c          560 g          358 t
ORIGIN      
        1 gcctcgcagt cggtctcgcc cgcggggaga atcccggcaa gctccacttc tttgtgaagc
       61 cccgattcgc cgatcaccca cgcccgcccg attccctctc cggcgaggta cttcgcggtg
      121 gccgacccgg aggtgacgat ctcgtccatc tccgccgcga cgccgtgccc gcgcagccgg
      181 tcggcgatgc gcaccctcgt ggtggggttg ttggtcagga atcggatcgt cttccccatg
      241 ctccgcaggg cgtcgagaac cgacaccgct ccgggcgtcg tctcaccgcc gatgtagatc
      301 acaccgtcga gatcgaagaa aaaaacgtca aacatgtcgc cgatcatgag tatcctcctt
      361 cgccgcaatt actgagaaaa accggaaatc attgtaccat ttttccattt tttttggaaa
      421 ggcgcgtgcg attgccctcc ggttctttcc tataccttcg tttggtttat aatgcgttcg
      481 gggtgcagcc gtccagaaac ggctgccgag tatgtacagc gaaagggagg aatgaagatg
      541 agaaatgcgg tagcgtcgat agagacctat tacgagcggg gaatgggctt tctcttccgg
      601 ttgatcgacg agtgccccga cgagctctgg gggaagaagg gcggagggtt cttctactgg
      661 cagcagctgt atcatgcgtt ctactgcgtg gactacttcc tgctccccgc aggaagcgaa
      721 tttccggaga agccgtacgg cagcgcggtg gccatgttcc gcgagaccct cgacaccgtc
      781 ccggcgaagg atgaaattcg cgagtacggc gcgatgatga aggcgaaggc ggacgcatgg
      841 atcgccgctc tcgacgacgg catgctcggc gacaggcacg aaggcatgtc cgagcgacgg
      901 aagaccgaag tgaccaacgc gggtgtcctt acgtcgctgt gcggccacaa catgtaccat
      961 gttggctgcc ttgattctgt gctgcgggag aacgggctga agggcgtgta ttaagagggc
     1021 ggaagggagt ccgttgcggg actcccttct ttgaaaacta cgcctgtttc ttcagccagt
     1081 cgaaggccat cgtggccatg acggccggtc cgatccagag gacgtcctcg tcgatgttga
     1141 aacggctgtt gtggtgcggc cacgtctgcc ccttcgcctc gttgcagccg gggaggaaga
     1201 agaacgtgcc gggggccttt tcgaggaaga aggcgacgtc ctcgccgccc atcgaggggc
     1261 gggggatctc ccggactttc tcgggaccga cgatcttttc cgccgccagg cgaagctcct
     1321 ccgtcatgac ggggtcgttc accacgggcg aggggccgtg ccagccgtac gagaactcga
     1381 cgctcccccg cattccggag gccaccgccg aggccacctc gccgattcgc cgggcaagga
     1441 actcccgcat ctcctgattg aaggcccgga ccgtgccggt gatgcggcac tctccgggga
     1501 tgatgttgaa ggcgcttccc gcgtggatct cgcccaggct gatcacgcac ggctcctgag
     1561 ggtcgacctc gcggctgatg atcgtctgca actcgcagat gatctgccct gcgatagaga
     1621 tgggatctac ggagccttga gggtttgctc catgcgcccc tttccctttg atcagaatat
     1681 cgaagcggtc catgcaggcc atgattcctc cagggcggta gccgatctca ccggggacaa
     1741 aacccttcca gatggagccg gtatgcagcc cgaccatcgc gtccggcttc gggtc
//
LOCUS       DAOTIL010000623         1792 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminobacteriaceae bacterium isolate AMR_MDS_3026
            NODE_78110_length_1792_cov_2.676454, whole genome shotgun sequence.
ACCESSION   DAOTIL010000623 DAOTIL010000000
VERSION     DAOTIL010000623.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868050
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminobacteriaceae bacterium (wastewater metagenome)
  ORGANISM  Aminobacteriaceae bacterium
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae.
REFERENCE   1  (bases 1 to 1792)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1792)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:19:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,224
            CDSs (total)                      :: 3,198
            Genes (coding)                    :: 3,184
            CDSs (with protein)               :: 3,184
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1792
                     /organism="Aminobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_78110_length_1792_cov_2.676454"
                     /isolate="AMR_MDS_3026"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:3034609"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1402
                     /locus_tag="P5201_11535"
     CDS             <1..1402
                     /locus_tag="P5201_11535"
                     /inference="COORDINATES: protein motif:HMM:NF012731.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="DNA gyrase subunit A"
                     /protein_id="HRV99219.1"
                     /translation="FSLRVPLVDGQGNFGSLDGDSPAAMRYTEVRLAPEGELMHADVD
                     EETVNFVPNFDESLTEPSVLPAVLPNLLINGASGIAVGMATNIPPHNPVETLSLLSEY
                     LRDGCSWSVSETASRMPGPDFPTGGEIVGTTGIREMYETGRGKFTLRGRTHIEETKNR
                     YLVVITEIPYGATKSRIVAQIAEKLDEKNVEGIVTVRDESDRTGDRVVVEMQKKTDPK
                     AVEALMHSATQLSGTFGGNLLALAGGAPRTMTLKDIFDCFVGHRREIVRRRTEFRLKR
                     DEARLHIVEGLLKALDILDEIVALIRASKSVAEAKAGLVDRFGFSVLQAEAILEMRLS
                     RLVGLEYEALVKEKKKLEKAIAEWKGILASAKKLDSVVAGELDELAARFAKSPLAARR
                     TSILDEEPEKKRGKDAPGQARLSFEQPSPCVISLDAEGYIRKREMKRRPRDEEEGSVF
                     EMALRTVAEAFSEKAP"
     gene            1499..>1792
                     /locus_tag="P5201_11540"
     CDS             1499..>1792
                     /locus_tag="P5201_11540"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRV99220.1"
                     /translation="MQQREERKRRRDRTCQARADKPAHRQAGEEHHHHASEADGERGA
                     EVGLEQHERHRQADQPGGRQQTTPVRGHRGGQVAVESREDEHDVGHIRLAREAD"
BASE COUNT          343 a          556 c          628 g          265 t
ORIGIN      
        1 cttctcgctc cgcgtcccgc tcgtggacgg acagggaaac ttcggctcgc tcgacggcga
       61 ctctcccgcg gcgatgcgct acacggaggt ccgcctggcg ccagagggag agctgatgca
      121 cgccgatgtc gacgaggaga cggtgaactt cgtcccgaac ttcgacgagt cgctcaccga
      181 gccctccgtc cttcccgccg ttcttccgaa cctgctgatc aacggagcgt cgggaatagc
      241 ggtcggtatg gcgacgaaca tcccgccgca caaccccgtc gagacgctct cgttgctctc
      301 ggagtacctt cgggacggct gctcgtggag cgtctccgag acggcgtcga ggatgcccgg
      361 gcccgacttc cccacggggg gcgagatcgt cgggacgact ggcattcgcg agatgtacga
      421 gaccgggcgg ggcaaattca ccctgcgcgg caggacgcat atagaggaaa cgaagaacag
      481 gtacctcgtc gtgatcaccg aaattccgta cggcgccacg aagtcgcgca tcgtcgcgca
      541 gatcgccgag aagctcgacg agaagaacgt cgaggggatc gtgaccgtcc gggacgagtc
      601 cgacaggacg ggcgaccggg tggtcgtgga gatgcagaag aaaaccgatc ccaaggccgt
      661 ggaggcgctg atgcactcgg ccacgcagct ctccggcacc ttcggcggca acctgctcgc
      721 gctcgcgggg ggcgcgccgc ggacgatgac gctcaaggat atcttcgact gcttcgtcgg
      781 ccaccggcgc gagatcgtcc ggcgaaggac cgagttccgt ctgaagcgcg acgaggcgag
      841 gctgcacatc gtcgaggggc tgctgaaggc gctcgatatt ctcgacgaga tcgtcgcgct
      901 gatacgcgcc tcgaagagcg tcgccgaggc gaaggccggg cttgtcgatc gattcggctt
      961 ctcggttctc caggctgagg cgatcctcga gatgcgcctc tcacggctcg tcgggctgga
     1021 gtacgaggcg ctcgtcaagg agaaaaagaa gctggagaag gccatcgccg agtggaaggg
     1081 aattctcgcc tccgcgaaga agctggactc cgttgtggct ggtgagttgg acgagctggc
     1141 ggcgcgtttc gcgaagagcc cgctcgcggc gcggcggacc tccattctcg acgaggagcc
     1201 ggagaagaag cgcggcaagg acgcgccggg gcaggcgcgg ctctcgttcg agcaaccgtc
     1261 gccctgcgtg atctcgctcg acgccgaggg gtacatccgg aagcgcgaga tgaagcgcag
     1321 gccgcgcgac gaggaggagg ggagcgtctt cgagatggct ttgcgaaccg ttgccgaagc
     1381 cttttccgaa aaagccccct gaaccgatcg cgatcttcga ctgggtgatg tggtagcccg
     1441 aacccagcgg gtcgttttcc ggatcgagga aggtcgtcac gcggcggcgc tggtagtcat
     1501 gcagcagcgt gaagaacgca agcggcgccg cgatcgcacc tgccaggccc gcgccgacaa
     1561 accagcgcat cggcaggccg gcgaggaaca tcaccaccac gcctccgaag ccgatggcga
     1621 gcgcggtgcc gaggtcgggc tggagcagca cgaacgccat cggcaggccg accaacccgg
     1681 cggcaggcag caaaccacgc cagtgcgcgg tcatcgaggc gggcaggtcg ctgtagaatc
     1741 gcgcgaggac gagcacgatg tcggccatat tcgactggcc cgggaggctg ac
//
LOCUS       DAOTIL010000624         1791 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminobacteriaceae bacterium isolate AMR_MDS_3026
            NODE_78179_length_1791_cov_2.360023, whole genome shotgun sequence.
ACCESSION   DAOTIL010000624 DAOTIL010000000
VERSION     DAOTIL010000624.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868050
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminobacteriaceae bacterium (wastewater metagenome)
  ORGANISM  Aminobacteriaceae bacterium
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae.
REFERENCE   1  (bases 1 to 1791)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1791)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:19:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,224
            CDSs (total)                      :: 3,198
            Genes (coding)                    :: 3,184
            CDSs (with protein)               :: 3,184
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1791
                     /organism="Aminobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_78179_length_1791_cov_2.360023"
                     /isolate="AMR_MDS_3026"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:3034609"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..960
                     /gene="pglZ"
                     /locus_tag="P5201_11545"
     CDS             <1..960
                     /gene="pglZ"
                     /locus_tag="P5201_11545"
                     /inference="COORDINATES: protein motif:HMM:TIGR02687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BREX-1 system phosphatase PglZ type A"
                     /protein_id="HRV99221.1"
                     /translation="TENRIKILSKANGLRATACKTDELMAMKGDDCRALVREHDVLFV
                     YHNRIDAVGDKRESEERVFEAAEESLLEIVRLVKKLTGANVNNILVTSDHGFIYQNRA
                     LDESDFFGGEAKGERILYRDRRFVLGKGLLPSPGLHTFTSARLGLSGGVEVQIPKSIN
                     RLRLKGSGSRFVHGGASLQEVVVPVLRINKRRTSDLSSVEVEIVRGGSALITSGQIAA
                     TLYQTEAATEKVQPRHLRAGIFTESGELISDSHDLTFDLTSENPREREQQVRFLLTRK
                     ADEANGRDVYLRLEEKVPGTSYFKEYTSLRYTMRRSFTSDFDF"
     gene            978..>1791
                     /gene="brxL"
                     /locus_tag="P5201_11550"
     CDS             978..>1791
                     /gene="brxL"
                     /locus_tag="P5201_11550"
                     /inference="COORDINATES: protein motif:HMM:TIGR02688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004176 - ATP-dependent peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0030163 - protein catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BREX system Lon protease-like protein BrxL"
                     /protein_id="HRV99222.1"
                     /translation="MNELDKKINAHFPGLVVRKDLVKTVKGNAIVPTYVLEYLLGQYC
                     ATSDEPTIQAGIETVREILRKHYVHRGEAGLVRSNIKEKGRYKVIDKISVALNDKTDA
                     YEAEFSNLGIRKVLVDSGTVKAHPKLLVGGVWCIADVEYEFTEAKNASPWILSMLKPI
                     QLSHFDFDAYAAARRQFSTDEWIDLLIQSVGFNPELFGKRSKLTHLLRLIPFCERNYN
                     LIELGPKGTGKSHIYSEFSPHGILLSGGEVTVPKLFVNNSNGKIGLVGYWDCV"
BASE COUNT          384 a          514 c          568 g          325 t
ORIGIN      
        1 acggagaacc gcatcaagat cctttcgaag gcgaacgggc tgcgggcgac tgcctgcaaa
       61 acggacgagc tgatggccat gaagggcgac gactgcagag cgctagtgcg cgagcacgat
      121 gtgctcttcg tctaccacaa ccggatcgac gccgtcggcg acaagcgcga gtcggaggaa
      181 cgggttttcg aggcggcgga ggagtcgctg ctggagatcg ttcgtctggt gaagaagctg
      241 acgggcgcca acgtgaacaa catactggtc acctcggacc acggcttcat ctaccagaac
      301 cgggccctcg acgagagcga cttcttcggg ggcgaggcga agggggagcg aattctttac
      361 agagaccgcc gtttcgtcct cgggaagggg ttgctcccct cgccgggact gcatacgttc
      421 acttctgcgc ggctcggact ctccggcggg gtggaggtgc agattcccaa gtcgatcaac
      481 cgtttgcggc tgaagggctc ggggagccgt ttcgtccacg ggggcgcttc gctgcaggag
      541 gtggttgttc cggtgctccg tatcaacaag agacggacga gcgacctctc ctccgtcgaa
      601 gtggagatcg tgcgcggggg cagcgcgctc atcacctccg gtcagatcgc ggcgacgctc
      661 taccagacgg aggcggcgac ggagaaggtg cagccgcgcc acctcagggc gggcatcttc
      721 acggagtcgg gcgagctgat ctcggacagc cacgacctga ccttcgacct gacttcggag
      781 aaccctcgcg agcgagagca gcaggttcgg tttctgctca cccgcaaggc cgacgaggca
      841 aacggcaggg atgtgtatct cagactcgag gaaaaggttc ccggcacttc gtatttcaag
      901 gagtacacgt cgcttcgcta tacgatgcgc agatcgttta ccagcgactt cgacttttaa
      961 aaaagagggt gtgcacgatg aacgaactcg acaagaagat caacgcgcac ttcccgggac
     1021 tcgtcgtccg caaggatctc gtcaagacgg tcaaggggaa cgcgatcgtt ccgacctacg
     1081 ttctggagta cctgctgggc cagtactgcg cgacaagcga tgaaccgacc attcaggcgg
     1141 gcatcgagac agtccgggag attctgcgga agcactacgt ccatcgcggc gaggcggggc
     1201 ttgtccgctc caacatcaag gaaaagggac gctacaaggt catcgacaag ataagcgtcg
     1261 cgctgaacga caagaccgac gcgtacgagg cggagttctc gaacctcggc atcaggaagg
     1321 tgctggtgga ctccggaacc gtcaaagccc atccgaaact tctggtcggc ggcgtctggt
     1381 gcatcgccga cgtggagtac gaattcaccg aggcgaagaa cgcctctccg tggatattgt
     1441 cgatgctgaa gccgatacag ctctcccatt tcgatttcga cgcctacgcc gccgcgagga
     1501 gacagttttc cacggacgag tggatcgacc tgctgattca gagcgttggc ttcaatcctg
     1561 agctgttcgg caagcgcagc aaactgaccc atctgctccg tctgattccc ttctgcgagc
     1621 ggaactacaa cctgatagaa ctgggaccga aggggaccgg aaagtcacat atctactcgg
     1681 agttctcccc ccacggcatt ctgctctcgg ggggcgaggt gaccgttccg aagctcttcg
     1741 tgaacaactc gaacgggaag atcggtctgg tcggctactg ggactgcgtc g
//
LOCUS       DAOTIL010000625         1790 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminobacteriaceae bacterium isolate AMR_MDS_3026
            NODE_78217_length_1790_cov_3.349280, whole genome shotgun sequence.
ACCESSION   DAOTIL010000625 DAOTIL010000000
VERSION     DAOTIL010000625.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868050
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminobacteriaceae bacterium (wastewater metagenome)
  ORGANISM  Aminobacteriaceae bacterium
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae.
REFERENCE   1  (bases 1 to 1790)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1790)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:19:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,224
            CDSs (total)                      :: 3,198
            Genes (coding)                    :: 3,184
            CDSs (with protein)               :: 3,184
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1790
                     /organism="Aminobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_78217_length_1790_cov_3.349280"
                     /isolate="AMR_MDS_3026"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:3034609"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1672
                     /gene="fusA"
                     /locus_tag="P5201_11555"
     CDS             <1..1672
                     /gene="fusA"
                     /locus_tag="P5201_11555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008521539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HRV99223.1"
                     /translation="FVNKMDRVGADFENVVRGIKEKLGAKPLPVQIPIGVEDSFTGMI
                     DLVEFHGIQYTDDMGTDPHVVPIPSALADEAKAARESMLETLSDYDEQIMEAFLDGGD
                     VPVETIKRAIRAATVALKLVPVICGSAFKNKGVQFLLDAVVDYLPSPVDLPPIKGTEP
                     ATEEEVLRQSSTEESFTALAFKIAVDPFVGRLVFTRIYSGVLSKGTAIYNSSTHSRER
                     VGRILRMHANKREELDEATAGMIVALPGLKNTKTGDTLCEEDKPLLLEKLVFPEPVIH
                     LSVEPATKNDKIKLTKGLSALSEEDPTFRAYTDEETSQTIISGMGELHLEIIVDRLKR
                     EFGVDVKVGRPQVAYREAIKHAARAEGKYVRQSGGRGQYGHVVFEVEPLEEGKGFEFE
                     DKIVGGVVPKEYINAVSKGLEEALNNGVLGGYPVIGIKVALVDGSYHEVDSSEMAFKI
                     AASMGFKEAMRKAGPVLMEPIMSVEVVTPEDYVGDVIGDLSSRRGRIDGMDMRMNTRI
                     VRAFVPLAEMFGYSTDLRSKTSGRAAYSMQFHHYEAVSSDVADKVLKG"
BASE COUNT          427 a          487 c          523 g          353 t
ORIGIN      
        1 cttcgtgaac aagatggatc gtgtcggggc cgattttgag aacgtcgtcc ggggcatcaa
       61 ggaaaagctt ggagccaagc cgcttcccgt ccagattccg atcggcgtcg aggacagctt
      121 tacaggaatg atcgatcttg tcgaatttca tggtatccag tacacggacg atatgggaac
      181 cgatcctcac gttgtaccta ttccttcagc gcttgccgac gaggcaaagg ccgcgcgtga
      241 atccatgctg gagactcttt cggattatga cgagcagatc atggaagctt ttctcgacgg
      301 gggggacgtc cctgtagaaa cgatcaagag agcaattcgc gccgctaccg tcgcactgaa
      361 gctcgttccg gtcatctgcg gatcggcgtt caagaacaaa ggggttcagt ttctgctcga
      421 cgctgttgtg gactatcttc cgagtccggt ggaccttccc cccatcaagg ggacggaacc
      481 cgcgacggaa gaggaggtcc tcagacaaag cagcaccgag gagtccttca ctgcgcttgc
      541 attcaaaatt gcggttgatc ctttcgtcgg acggctcgtc tttacgcgca tctattccgg
      601 cgtcctttcg aaggggacgg ccatctacaa ctccagcaca cacagccgcg agcgcgtggg
      661 gcgcatcctg aggatgcatg ccaacaagcg ggaagagctg gacgaagcca ccgcaggcat
      721 gatcgtcgcg cttccggggc tgaagaacac gaagaccgga gataccctct gcgaagagga
      781 caaaccgctt cttcttgaga agctggtttt ccccgagccg gttatccacc tctcggtcga
      841 acccgcgacg aagaacgaca agatcaagct gacgaagggg ctctcggcgc tgtccgaaga
      901 agaccccaca ttccgcgctt atacggacga ggaaacctct cagaccatca tttccggcat
      961 gggcgagctt catctggaga tcatcgtcga ccgtctcaaa agagagttcg gcgtcgacgt
     1021 caaggtcgga agacctcagg tcgcctaccg cgaagctatt aagcacgctg ccagagccga
     1081 ggggaaatat gtccgccagt cgggcggacg cggccagtac ggccacgttg tcttcgaggt
     1141 cgagccgctc gaagagggca aaggcttcga attcgaggac aagatcgtgg gcggcgtcgt
     1201 tcccaaggag tatatcaacg cagtctccaa gggacttgaa gaggcgctca acaacggcgt
     1261 cctcggcgga tacccggtga tcggcatcaa agtagcgctc gtcgacggaa gctaccacga
     1321 agtggacagc tccgaaatgg ccttcaaaat cgcagcctcc atgggattca aggaggctat
     1381 gcggaaggcg ggaccggtcc tcatggagcc catcatgtca gtcgaagtcg tcaccccgga
     1441 ggactacgta ggcgacgtca tcggcgacct ctcctcccgg aggggacgaa tcgatggaat
     1501 ggacatgcga atgaacacca ggatagtccg tgcattcgtt cctctcgccg agatgttcgg
     1561 gtattccacc gaccttcgga gcaagacttc aggacgcgca gcgtattcca tgcagttcca
     1621 tcactacgag gcggtcagtt ccgacgttgc cgacaaggtg ctcaagggct gaggaacgca
     1681 tatacttatt gattttgatt caaagcaata atgatttttc cgaggaggga attgaacaat
     1741 ggcgaaggag aaatttgaga ggtcgaagcc gcatttgaac ataggtacga
//
LOCUS       DAOTIL010000626         1787 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminobacteriaceae bacterium isolate AMR_MDS_3026
            NODE_78395_length_1787_cov_3.327945, whole genome shotgun sequence.
ACCESSION   DAOTIL010000626 DAOTIL010000000
VERSION     DAOTIL010000626.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868050
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminobacteriaceae bacterium (wastewater metagenome)
  ORGANISM  Aminobacteriaceae bacterium
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae.
REFERENCE   1  (bases 1 to 1787)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1787)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:19:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,224
            CDSs (total)                      :: 3,198
            Genes (coding)                    :: 3,184
            CDSs (with protein)               :: 3,184
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1787
                     /organism="Aminobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_78395_length_1787_cov_3.327945"
                     /isolate="AMR_MDS_3026"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:3034609"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..253
                     /locus_tag="P5201_11560"
     CDS             <1..253
                     /locus_tag="P5201_11560"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="type II toxin-antitoxin system VapC family
                     toxin"
                     /protein_id="HRV99224.1"
                     /translation="KKRLGQAASVRFLSLLGALPITVEQEPSERMLKEILSLARTLGL
                     STYDASYLDLAMRLDLPLATLDAALANAAKKSGVQIYRP"
     gene            484..>1787
                     /locus_tag="P5201_11565"
     CDS             484..>1787
                     /locus_tag="P5201_11565"
                     /inference="COORDINATES: protein motif:HMM:NF013995.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EVE domain-containing protein"
                     /protein_id="HRV99225.1"
                     /translation="MSTYISQEQKLKIQDEFKEYLKKEHPGWVERTVYTNVSDSFYVF
                     NNDIGMNPWSCFVSDALMEEAREVLTEYFRVHDKPGNPETRANGYIKSMQLLKLFLDR
                     NYPGLASEQSVKTRTPSCWIFQCNPKYYDLLRALREKETLRWSINQYAKRIQAGDKVY
                     LWMSGSDGGIVASGKVDSAPEKRAPDLNDAYWMDGYRPTEEYLCVDISLERKFADSVI
                     SRSVLSADERTKKLGILLFPQGTNFHVAREQEAVIESILEGAYVRVPATSDDATAEGK
                     RYWMYAPGKGAEKWDEFHKHGIMAVGWDELGDLSRYSSKQNIKEKMKEVYGEDKNYMN
                     DGHALWQFANDVKPGDVVFVKKGKNMIVGRGEVTSEYFFDESRAEYQHIHEIDWTHSG
                     EWSAEKDLPVKTLTDFTPYTEACRLLEEKFEGDVVIEDDPPKT"
BASE COUNT          466 a          476 c          499 g          346 t
ORIGIN      
        1 gaaaaaacga ctcggtcagg ctgcgtccgt tcgctttctc tccctcctgg gcgcgctgcc
       61 gatcactgtg gaacaggaac cctccgaacg catgttgaaa gaaatccttt cgctcgcccg
      121 taccctcgga ctgtccacct acgacgcatc ctacctggat cttgccatgc ggctcgatct
      181 tccgctcgcc acgctggacg ctgccctcgc gaatgcggcg aagaaaagcg gagtacagat
      241 ctacaggccg taacggcgaa gagtgctccc ggaagcggag gccggacgaa atacttgaca
      301 ataagacttc aacgtatata ttggactgtg gaaaacgagg ctctccgttg cattctgcga
      361 gcgtctcaat acagctcctc acatgatgaa aaacaggtcg ggataccaat gccggatacg
      421 gaaaaatcag ggcatcgccg gcgtttgcgg gagcgcttcg ccctgaatga gggaggcaca
      481 cgtatgagca cctatatctc acaggagcaa aagctgaaaa tccaagacga gttcaaagaa
      541 tatctgaaaa aagaacatcc gggatgggtt gaaagaacag tgtatacgaa cgtcagcgac
      601 tccttctatg tcttcaataa cgatatcggg atgaaccctt ggtcctgctt cgtcagcgac
      661 gccttgatgg aagaggcccg ggaagtgctc acggagtatt ttcgagtaca tgacaagccg
      721 ggaaatcccg aaacacgcgc gaacggctat atcaaatcca tgcaactcct caagctgttc
      781 cttgatcgca actaccccgg cctcgcctcc gaacagtctg taaaaacacg caccccctcc
      841 tgctggatat tccagtgcaa ccccaaatac tacgaccttc ttcgggccct gcgggaaaag
      901 gagacgctcc gctggagcat caatcagtac gcgaaacgga ttcaggccgg agacaaggtg
      961 tatctctgga tgtccggttc cgacggaggc atcgtcgcct cggggaaggt agattcagct
     1021 ccggagaaaa gggcgcccga tttgaacgac gcttactgga tggacggtta tcgcccgacc
     1081 gaggaatatc tgtgtgtcga catttctttg gaacgcaagt tcgccgactc tgtcatttcc
     1141 agaagcgttc tttccgccga cgagcgaacg aaaaaactgg ggatcctgct cttcccgcag
     1201 ggaacgaact ttcacgtcgc tcgggagcag gaggccgtca tcgagagcat actggaaggc
     1261 gcctatgttc gtgtaccggc aacgagcgac gacgccaccg cggaagggaa gcggtactgg
     1321 atgtacgctc caggcaaagg agcggagaag tgggatgaat tccataaaca cgggatcatg
     1381 gcggtcggtt gggacgaact gggcgatctt tctcggtact cctccaaaca aaacatcaag
     1441 gagaaaatga aagaggttta cggagaggat aagaactaca tgaacgacgg ccatgcgctc
     1501 tggcagttcg ccaacgatgt gaagccggga gacgtcgtct tcgttaaaaa ggggaagaac
     1561 atgatcgtcg ggcgcgggga ggtgacttcg gagtatttct tcgatgaatc ccgcgccgaa
     1621 taccagcata ttcacgagat cgactggacg cattcgggtg aatggagcgc cgaaaaggac
     1681 ctgcccgtca aaacgctcac cgacttcacg ccctacaccg aagcgtgtcg gctgttggaa
     1741 gagaagttcg aaggggatgt agttatcgag gacgatccgc cgaaaac
//
LOCUS       DAOTIL010000627         1786 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminobacteriaceae bacterium isolate AMR_MDS_3026
            NODE_78477_length_1786_cov_2.532640, whole genome shotgun sequence.
ACCESSION   DAOTIL010000627 DAOTIL010000000
VERSION     DAOTIL010000627.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868050
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminobacteriaceae bacterium (wastewater metagenome)
  ORGANISM  Aminobacteriaceae bacterium
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae.
REFERENCE   1  (bases 1 to 1786)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1786)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:19:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,224
            CDSs (total)                      :: 3,198
            Genes (coding)                    :: 3,184
            CDSs (with protein)               :: 3,184
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1786
                     /organism="Aminobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_78477_length_1786_cov_2.532640"
                     /isolate="AMR_MDS_3026"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:3034609"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            107..1342
                     /locus_tag="P5201_11570"
     CDS             107..1342
                     /locus_tag="P5201_11570"
                     /inference="COORDINATES: protein motif:HMM:NF037653.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HRV99226.1"
                     /translation="MNGSSEKLSFAPFFKAIGGRQDVRAVLDVAAEYLKTGAAFREAY
                     TGNVSVVSSSEIFRESAKTFPILELSRIYCSREVQVGNVHSGILIFDIPSDRAHALSD
                     EESIYIENAVIATALCLERSFSRTLPGNEKRDELLKDLLAGEVRDYREAEESFAQWGW
                     KKDQEFLVLVLRLADDVKEDDGFSARERHISFSLARSKVLPFFPKSFCVFYTKMPVFL
                     LPLPSGKPEALSGVKDVLESILLSLKDELPPKKGRAFLFAAGGAKRGVNAIHRSFDEA
                     VRVLSVSESLRREGGVILWDMLGGYQLVAILSETEEARLFCGRILGELLEGTDSSSVE
                     LLETLLVMDGCNWNVRAASESLRFHYNTVKYRLGVLRERIGFDSSDPAQRFDMGLALR
                     LAPLFFKDKFVTICQKPFI"
     gene            1413..>1786
                     /locus_tag="P5201_11575"
     CDS             1413..>1786
                     /locus_tag="P5201_11575"
                     /inference="COORDINATES: protein motif:HMM:NF013696.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HRV99227.1"
                     /translation="MPVPLDTSRALEILRQLIRIRTVQPYGDEKDAVGYILSLFPEEC
                     IEKIVVDHGDNRASLVLVVPGEDRSRKIAVSGHLDTITVGERSLWTKAPFGAETDGTR
                     VYGRGAADMKGGLTSMLLSARA"
BASE COUNT          374 a          449 c          535 g          428 t
ORIGIN      
        1 tcgggcagat agcgtgaagg ataagccccc ctgccggaag gccggggggc tttcggtttc
       61 aggagaggcg tttgaaaaac gcgtgcatgc ggggaggtga cattgaatga acggttccag
      121 tgaaaaactg tcttttgctc cattcttcaa agcgattggg ggcaggcaag atgtccgggc
      181 cgttctcgat gttgccgccg agtatctgaa gacgggagca gccttccggg aggcgtacac
      241 gggaaacgtc agcgtcgtct cctcttccga aatctttcgg gaaagtgcga agacgtttcc
      301 gattcttgag ctgtccagaa tatactgttc ccgcgaggtg caggtcggga atgtccactc
      361 cggtatcctc atcttcgaca ttccgtcgga ccgcgcccac gctctttcag acgaggagtc
      421 gatctatatc gagaacgccg tgatcgcgac ggcgctctgc ctggagcgtt ctttcagccg
      481 cacgcttccc gggaatgaga aaagggacga actcttgaaa gatttgctcg ccggggaggt
      541 gcgcgactac agggaagcgg aggagtcgtt tgcgcaatgg gggtggaaaa aggaccagga
      601 gtttcttgtg ctggttcttc gtcttgcgga cgatgtaaag gaagatgatg ggttttcggc
      661 gagggagcgt catatttcct tctcacttgc acgaagcaag gtgctccctt ttttccccaa
      721 gtctttttgt gttttttata caaagatgcc ggtgtttctc cttccgctcc cctccggaaa
      781 accggaggcc ctttccggag tgaaagatgt tcttgagagc atactccttt cgctgaaaga
      841 cgagcttccg ccgaagaaag gacgcgcttt tctctttgcg gccggaggcg ccaagagagg
      901 tgtgaacgca attcaccgga gcttcgacga agcggtacga gtgctttcag tctcagaaag
      961 cctccggaga gaagggggcg tcattctttg ggatatgctc ggcggatacc agctggttgc
     1021 gatcctctcc gaaaccgagg aggcgcgcct tttttgcggc cggatcctgg gcgaactgct
     1081 ggagggaacc gattcttcct cagtggaact tctggagacc ttgctggtga tggacgggtg
     1141 caactggaac gtcagggcgg cgtccgagtc gctccgtttt cattataata cagtgaagta
     1201 ccgccttggt gttctgcggg agcggattgg atttgactct tccgacccgg cgcaacgctt
     1261 tgatatgggg cttgcccttc gacttgcgcc gttatttttt aaagataaat ttgttactat
     1321 ttgccaaaaa ccttttatct gatttggccg aaccggataa tgaaaaaaaa gatgtttccc
     1381 cgtatccttg agatactcaa ggaggggata gaatgcctgt tcctctggat acctcgcggg
     1441 ccctggaaat actgcgtcag ctcatccgta ttcggacagt gcagccctac ggcgatgaaa
     1501 aggatgccgt cgggtacatc ctctctctct ttcccgaaga atgtatcgaa aaaatagtgg
     1561 tcgatcacgg agataaccgt gcatccctgg tgcttgttgt gccgggggag gaccgctcac
     1621 gaaagatagc cgtttccggt cacctggaca cgatcaccgt gggtgagcgc tcgttgtgga
     1681 cgaaggcgcc gttcggcgcg gaaaccgacg gaacgcgcgt ctacggaaga ggtgcagccg
     1741 atatgaaggg cgggctcacc tccatgcttc tctccgcgcg cgcctg
//
LOCUS       DAOTIL010000628         1777 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminobacteriaceae bacterium isolate AMR_MDS_3026
            NODE_78946_length_1777_cov_5.154472, whole genome shotgun sequence.
ACCESSION   DAOTIL010000628 DAOTIL010000000
VERSION     DAOTIL010000628.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868050
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminobacteriaceae bacterium (wastewater metagenome)
  ORGANISM  Aminobacteriaceae bacterium
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae.
REFERENCE   1  (bases 1 to 1777)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1777)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:19:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,224
            CDSs (total)                      :: 3,198
            Genes (coding)                    :: 3,184
            CDSs (with protein)               :: 3,184
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1777
                     /organism="Aminobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_78946_length_1777_cov_5.154472"
                     /isolate="AMR_MDS_3026"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:3034609"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1376
                     /locus_tag="P5201_11580"
     CDS             <1..1376
                     /locus_tag="P5201_11580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013047580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016625 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, iron-sulfur
                     protein as acceptor [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="aldehyde ferredoxin oxidoreductase C-terminal
                     domain-containing protein"
                     /protein_id="HRV99228.1"
                     /translation="ACIGPAGENQVLLANIMHDGRNARAAGRGGMGAVMGSKNLKAIV
                     ARGSAKVLMADEKAVREKSAEKAKYYMEKLPAMTRFGTAGGVALAEQNGDLPLKNWSM
                     GSWTEDVKSITGEAMAETILTGNWGCMSCPIRCGRDVEFDGIKGAGPEYETIGMLGSN
                     CLISDLKAIARGNDLCNRMGIDTISGGSAVAFAMELYERGIISEEEIGYPLLWGDGES
                     MIRLLSDIVEKRGFGALLSEGTKKAAERIGKGAERYAIHSKGMDFPAHDPRCYKSLAT
                     GYATSNRGACHLSGFSYSFERSMTYPEFGVDTVMDRTVDEGKGAVNIPFQNMMGVLDS
                     LKMCKFPFAITRIDDILQWINGITGWNMSGDELLLAGERIFNLKRLFNVACGASALDD
                     TLPERILTEPRGSGGSANTLPDLKLQLKEYYPERGWSEDGIPTEEKLKSLDLEEFLCC
                     VNGPALE"
     gene            1403..>1777
                     /locus_tag="P5201_11585"
     CDS             1403..>1777
                     /locus_tag="P5201_11585"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRV99229.1"
                     /translation="MVHKIVRYSVLLFILVPLLTWFASRGADMVALARWNTEDSAIVI
                     LEKVLGREDDVESGAEGNWETERVHIRITYRNGAFAGTTEEIEILQLADSRLELVPGR
                     EYLFLVDTFDDGTHQYSISDRYR"
BASE COUNT          442 a          391 c          540 g          404 t
ORIGIN      
        1 ttgcctgtat aggtcctgca ggagagaatc aggtcttgct tgcaaatatc atgcacgacg
       61 gtcgaaatgc cagagctgcg ggaagaggcg gtatgggggc ggtaatgggc tccaaaaatt
      121 tgaaagccat agttgcccgc ggcagcgcaa aagtgctcat ggcggacgaa aaggctgtcc
      181 gagagaagtc tgcagaaaag gcgaagtact atatggaaaa gcttcccgcg atgacccgtt
      241 tcggtaccgc cgggggagtt gcgttggctg agcagaacgg agatcttccc ctgaagaact
      301 ggagtatggg ctcctggacg gaagatgtga agtccattac cggtgaagca atggccgaga
      361 cgatactgac cggcaactgg ggctgcatgt cctgcccgat acgatgcggc cgggatgtcg
      421 agttcgacgg aataaagggc gccgggccgg agtacgaaac gatcggtatg ctgggaagca
      481 actgcctcat cagcgatctg aaggccattg cgcgtggaaa cgacctgtgt aaccgaatgg
      541 gtatcgacac gatttcagga ggttcggctg tcgctttcgc catggagttg tacgaaagag
      601 ggataatttc ggaagaggaa attggctacc cgcttttgtg gggtgacggg gagtcaatga
      661 tacgtcttct atcggatatt gtagaaaagc gaggattcgg cgcgctgctg tccgagggaa
      721 ccaagaaggc tgcggagcgt atcggaaaag gggcggaacg gtatgccatt cattccaagg
      781 gaatggattt tcccgcgcac gacccccgct gttataagag cttggcaacg ggatacgcaa
      841 catcgaaccg gggagcctgc catctttccg gtttttccta ttcgtttgaa cggagcatga
      901 cctatccgga gttcggcgtt gatacggtga tggataggac ggtcgatgaa gggaaaggtg
      961 ctgtgaatat tcccttccag aacatgatgg gtgttcttga ttctttgaaa atgtgcaagt
     1021 tcccgttcgc aatcacaagg atagatgata ttcttcaatg gatcaacgga atcacggggt
     1081 ggaatatgag cggcgatgaa ctccttcttg cgggagagag gattttcaac ctcaagcggc
     1141 ttttcaatgt agcctgcgga gcctcggcgc tggacgatac tcttccggag agaattctta
     1201 cggaacctcg cggaagcggc ggttcggcga acactcttcc ggatctgaag ttgcagctca
     1261 aagagtatta tcctgaaaga ggctggtctg aagatggtat tcctaccgag gaaaaactga
     1321 aatctcttga tttggaagag ttcttatgct gtgtaaacgg gcctgctctc gaatgatacg
     1381 ctttggaaaa aaggagtgaa ttatggtaca taagattgtc cgttactccg ttctattgtt
     1441 tattctcgta ccgctgctca cctggtttgc gtcgcgagga gccgacatgg ttgcgctcgc
     1501 caggtggaat acggaagatt ccgccatcgt catccttgaa aaggtactcg gcagggagga
     1561 cgacgttgaa agtggagccg aaggcaactg ggagacagag cgcgtacaca tacgaattac
     1621 ctaccgtaac ggtgcgttcg ccggcacgac tgaggagatc gaaatccttc agcttgcgga
     1681 tagccggctg gagcttgttc ctgggagaga atacctcttt cttgtcgata cgttcgacga
     1741 cggaacacat cagtattcta taagcgacag atacagg
//
LOCUS       DAOTIL010000629         1777 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminobacteriaceae bacterium isolate AMR_MDS_3026
            NODE_78959_length_1777_cov_3.592334, whole genome shotgun sequence.
ACCESSION   DAOTIL010000629 DAOTIL010000000
VERSION     DAOTIL010000629.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868050
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminobacteriaceae bacterium (wastewater metagenome)
  ORGANISM  Aminobacteriaceae bacterium
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae.
REFERENCE   1  (bases 1 to 1777)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1777)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:19:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,224
            CDSs (total)                      :: 3,198
            Genes (coding)                    :: 3,184
            CDSs (with protein)               :: 3,184
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1777
                     /organism="Aminobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_78959_length_1777_cov_3.592334"
                     /isolate="AMR_MDS_3026"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:3034609"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(272..1591)
                     /locus_tag="P5201_11590"
     CDS             complement(272..1591)
                     /locus_tag="P5201_11590"
                     /inference="COORDINATES: protein motif:HMM:NF019310.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HRV99230.1"
                     /translation="MHEKSALIREDERKSMEKRALAYRWVVWGVMVFAFMVVFFHRLA
                     AGVVKEDVTSAFSLSATAFGAMSSMYFYAYMAMQIPVGLLADSLGARITVSVGMFAAG
                     AGSILFGFAPSYFWLLAGRFLVGLGVATVFVCIMKIQSQWFRDREFATISGATTFIGN
                     AGGIFSQGPLAFLLTIVSWRSSFIAIGIATLGISLLCYRLIRNRPQDMGFPPLNEREM
                     LREEMEAEPFSVLKGLKTVLSVRGVRIATLFYFFNQAGLFAFLGTWGTPWLVHVFEMS
                     IATASSLMVMTVIGIMIGGISTGWISDKIGRRRLPMIIAASVHSVLWGCMIFPWQQPL
                     GVGALRCIFLLLGFTNTAFVLSWSVAKELTSKKYTGLAISVLNAAGFFAIAVTTSIMG
                     MVIDYYSFLPLEAAYRRAFSLGLICSVFSLLFALLIPETGFMKRKEK"
BASE COUNT          511 a          469 c          372 g          425 t
ORIGIN      
        1 ttgctatcgt taaaccattt cacagttccc tgactcatcc aaagacagcc tcctaaaact
       61 ttattaaatt ctgcggtcca tatcctgatg ccggactgca aatcaacaat ataggatttt
      121 aggccttatg tcaagctttt tttaaaactt ttccttttta cgttcgtatc gattcctttt
      181 ttacccccct cgagtctttg gcatgattaa aatgaaaagg cttttcgtga gatgaaatta
      241 aactaaattt catatagagt ccaccgcgcg tttatttctc ttttcttttc ataaaccctg
      301 tttctggaat aagaagagcg aaaagaagag agaaaaccga acaaatcaac cccaacgaga
      361 acgcccttcg atatgcggct tccaggggga ggaacgaata gtagtcgatc accattccca
      421 taatggatgt cgtcactgca atggcgaaaa aacccgccgc attgaggact gaaatcgcca
      481 atccggtgta cttttttgaa gtcaactctt ttgccacgga ccaggaaagc acgaatgcag
      541 tattggtgaa tccgaggagg agaaaaatac accgcagcgc cccgacaccg agcggttgtt
      601 gccatggaaa gatcatgcac ccccaaagca ctgaatgcac tgatgcggcg ataatcatgg
      661 ggagccgtct tctcccgatc ttgtccgaga tccatccggt agagataccg cctatcatga
      721 taccgatgac tgtcatcacc ataagggatg aagccgtcgc tatcgacatt tcaaagacat
      781 gcacaagcca tggagttccc cacgtcccga gaaaagcgaa caaacctgcc tggttgaaga
      841 agtaaaagag cgtcgcgata cgtacccccc tcacagagag cactgttttc aatcccttga
      901 gaacggagaa cggctctgct tccatttctt cacggagcat ctcgcgttcg ttcaacggcg
      961 ggaagcccat atcctgcgga cgatttcgaa tcaacctgta acatagaaga gaaataccca
     1021 aagtcgctat tccgatcgct ataaaacttg atcgccagga tactattgtg agaaggaaag
     1081 caaggggacc ttgcgaaaag attccgccgg cgttaccgat aaacgttgtt gcacccgaga
     1141 ttgtggcgaa ttctctatcc cggaaccact gggactgtat tttcattatg catacaaaaa
     1201 ctgtcgcgac gccaagccct accaagaaac ggccggcaag aagccaaaaa tacgacggag
     1261 cgaagccgaa aagtatcgat cccgctccgg cggcgaacat ccccaccgaa acggttatcc
     1321 tggctcccag ggagtccgcg agaagtccga ccggtatttg catggccata tatgcgtaaa
     1381 agtacatcga agacatggcg ccgaacgctg tcgcgcttaa tgaaaacgca ctcgttacat
     1441 cttccttgac gactccggcg gctaaccgat gaaaaaaaac taccataaat gcaaaaacca
     1501 ttactcccca tactacccat cggtaggcaa gcgcccgctt ctccatagat tttcgctcat
     1561 cctcacggat gagcgcactc ttttcgtgca tgtgaacaat tcctttcttc acgccccttc
     1621 gcaggtcggc gtattctacg cattcatttt acaatgctgt agaagataat gatagcatca
     1681 tacaattttt ccacacctct tttatcaata cgccgtaccc cttataatga accctgtaca
     1741 cgaggaacaa ggaggaactt ttcggtgatc catctaa
//
LOCUS       DAOTIL010000630         1775 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminobacteriaceae bacterium isolate AMR_MDS_3026
            NODE_79070_length_1775_cov_3.209302, whole genome shotgun sequence.
ACCESSION   DAOTIL010000630 DAOTIL010000000
VERSION     DAOTIL010000630.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868050
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminobacteriaceae bacterium (wastewater metagenome)
  ORGANISM  Aminobacteriaceae bacterium
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae.
REFERENCE   1  (bases 1 to 1775)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1775)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:19:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,224
            CDSs (total)                      :: 3,198
            Genes (coding)                    :: 3,184
            CDSs (with protein)               :: 3,184
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1775
                     /organism="Aminobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_79070_length_1775_cov_3.209302"
                     /isolate="AMR_MDS_3026"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:3034609"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..638)
                     /locus_tag="P5201_11595"
     CDS             complement(<1..638)
                     /locus_tag="P5201_11595"
                     /inference="COORDINATES: protein motif:HMM:NF012376.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HRV99231.1"
                     /translation="MRKTGIMSIMLLSSLLCPATLCFASDYAFTLDESGIPALAGERS
                     AIVVISDLHLGMDDRYAETQGNRAPLVDFLQKVRRAPNIKELVVAGDLVDEWFIPADT
                     ETYAGKTQKEFVQAVAANNKEVFDAFNSIILDGKIKVTYVPGNHDLLISSESIQTVFP
                     GMAEARDVRGLGTYTPDGHPEIAIEHGHRYNFFCAPDPISHKDIAPGSILPPG"
     gene            complement(690..1454)
                     /locus_tag="P5201_11600"
     CDS             complement(690..1454)
                     /locus_tag="P5201_11600"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRV99232.1"
                     /translation="MKRTDRIFALLLFFSLLLSGLPPLEASENADPSEWTFSTTSYVW
                     FSGLNGTLGVKGHKARVNLSFSDVLSNMDVSGMLTFQGHKGKQYFFFDGMYLDMSASE
                     QVSPQLFTDVGATTTRLQAAWGVRVKEWESSSLHVFGGLRYWNLKNELTFRTASAPLR
                     HFSESESWIDPLVGVRFATQLSPNLTFLAILDAGGFGIGSDFTWGGMLDLSWKLSERT
                     SLELGYRYLYVDYEKDGFVMDAYNDGIFIGFTWKLR"
     gene            1565..>1775
                     /locus_tag="P5201_11605"
     CDS             1565..>1775
                     /locus_tag="P5201_11605"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRV99233.1"
                     /translation="MFKAFFMFNVFLYVFFFCADAGVMRRRSLKAPAAFRDHFRCCKM
                     VFFIRRIQRLVSPESEKLTGGESMQG"
BASE COUNT          360 a          475 c          540 g          400 t
ORIGIN      
        1 cccggaggca ggatcgagcc gggagcgatg tccttgtgcg agatgggatc cggcgcgcag
       61 aagaagttgt agcggtggcc gtgctcgatg gcgatctcgg ggtgtccgtc cggggtgtag
      121 gtaccgagtc ctcgaacgtc gcgcgcttcc gccattccgg ggaagacggt ctggatgctc
      181 tcggaggaga tcagcaggtc gtgattcccc ggaacatagg tcaccttgat cttcccgtcc
      241 agtatgatgc tgttgaaggc gtcgaacacc tctttgttgt tcgcggcgac ggcctgaacg
      301 aactccttct gggtctttcc ggcgtacgtc tccgtatcgg cggggatgaa ccattcgtcg
      361 acgaggtcgc ccgcgacgac gagctctttg atgttcggcg cgcgccggac tttctgaaga
      421 aagtcgacga gcggagcgcg gtttccttga gtctcggcgt accggtcgtc cattcccagg
      481 tggaggtcgc tgatgacgac gatagcggac cgttcgccgg cgagcgccgg aatgccgctc
      541 tcatcaaggg tgaaggcgta gtcggacgcg aagcatagag ttgcggggca cagcagcgaa
      601 gacaacagca tgatactcat gatacccgtt tttctcatag tctcattccc tttcgtgcgt
      661 gatatgatcg gtaaaagcgc gacggcgctc taccgaagtt tccacgtgaa gccgatgaag
      721 atgccgtcgt tgtatgcgtc catcacgaag ccgtccttct cgtaatcgac gtagagatag
      781 cggtagccca gctcgagcga cgtccgttcc gagagcttcc acgagagatc cagcattcct
      841 ccccaggtga agtccgaccc gatgccgaag ccgcccgcgt cgagtatggc gagaaaggtc
      901 aggttcggcg aaagctgtgt cgcgaagcgg acgccgacga gggggtcgat ccaggattcg
      961 ctctcgctga agtgccggag cggggccgac gccgtcctga acgtcagctc gtttttgagg
     1021 ttccagtatc tcaggccgcc gaaaacgtgg agggaagacg actcccactc cttgacgcgc
     1081 actccccatg cggcctgaag ccgggtcgtg gtcgcgccga cgtccgtgaa caattggggg
     1141 gatacctgct cgcttgccga catgtcaagg tacataccgt cgaaaaagaa gtactgcttt
     1201 cccttgtgcc cttgaaaggt gagcattccc gagacgtcca tgttcgaaag aacgtcggag
     1261 aagctcaggt tgacgcgcgc cttgtgcccc ttcacgccca gcgttccgtt gaggccggag
     1321 aaccagacgt acgacgtcgt ggaaaaggtc cactcggagg gatcggcgtt ctccgaggcc
     1381 tcaagcggag gaagtccgga aagaagaagg gagaagaaga gaagcaaagc aaaaatacgg
     1441 tccgttcgtt tcataaacgt cactccctct gcactttttt tgttcgctga gcagtgcgcg
     1501 ctctcgtgcg ctctttgtgc gcacaatcct ataaactata cgctatgtgc gtgattttgt
     1561 ccatatgttc aaagcctttt ttatgtttaa tgtattttta tatgtattct ttttttgtgc
     1621 ggacgcgggg gtgatgcgca ggcgctctct aaaagcgcct gccgcctttc gcgatcattt
     1681 ccgctgttgt aagatggtgt tctttatcag gcgcattcag cggcttgttt cccctgagag
     1741 cgaaaaattg acaggaggtg agtctatgca aggaa
//
LOCUS       DAOTIL010000631         1774 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminobacteriaceae bacterium isolate AMR_MDS_3026
            NODE_79125_length_1774_cov_3.346713, whole genome shotgun sequence.
ACCESSION   DAOTIL010000631 DAOTIL010000000
VERSION     DAOTIL010000631.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868050
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminobacteriaceae bacterium (wastewater metagenome)
  ORGANISM  Aminobacteriaceae bacterium
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae.
REFERENCE   1  (bases 1 to 1774)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1774)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:19:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,224
            CDSs (total)                      :: 3,198
            Genes (coding)                    :: 3,184
            CDSs (with protein)               :: 3,184
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1774
                     /organism="Aminobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_79125_length_1774_cov_3.346713"
                     /isolate="AMR_MDS_3026"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:3034609"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            330..881
                     /locus_tag="P5201_11610"
     CDS             330..881
                     /locus_tag="P5201_11610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005659668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArsR family transcriptional regulator"
                     /protein_id="HRV99234.1"
                     /translation="MLESLVTSKTRARLLLKFFLNPNTSAYLRGLAEEFGESTNGVRV
                     ELNRLSEAGLLECADEGRTKVYRANTRHPLFPELQRIAAKTLGIDQVVEHVVSRLGNV
                     ELALVTGDYAKGIDSGLIDLVLVGRIDKMYFESLAEKIEGIMQRKIRPLFLTREEFLR
                     LKERIVSENALLLWGDEGEIAAK"
     gene            complement(982..1623)
                     /locus_tag="P5201_11615"
     CDS             complement(982..1623)
                     /locus_tag="P5201_11615"
                     /inference="COORDINATES: protein motif:HMM:NF014628.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactate utilization protein"
                     /protein_id="HRV99235.1"
                     /translation="MSEFTPWHNETVGGAVVEALKKNGFEAEYCATGADAAQRLLELI
                     PEAASVGFGGSWTLKAIGISEKLAERGNTILDHGAPGLTNEEKADIRRKQQICDVFLS
                     GTNALTMDGRLVNMDGSGNRVAAMIFGPGKVIVVAGTNKIVKDLAAAEERIQMKAAPI
                     NNKRLGTPNPCSRTGVCMDCQGPTRICNVLTIISKRPLGTNFHVLIVGEELGF"
BASE COUNT          337 a          494 c          538 g          405 t
ORIGIN      
        1 gggagagcag gtcgccgccg gaaatccttt tgaaagaaca caggaagccg tccaggggaa
       61 aaccccaggg atggcttcct gtgtatgtgc cgtctattga ccgcgtgagt atacgacaac
      121 gacgttctcc gggactttca catgacgaag agccgccccg gaggaaatcc tcaaggggcg
      181 gctcttcgtg tttggagacg ttcgccgtga tgtgcgcgcg ctcatctttc cggaattgct
      241 tcgcttttgc gaatcagctt tccagcgttt gacaatcccc ctttcgagca ataaaattac
      301 tctaaataca tcacaaaaaa ggacgaagca tgcttgaatc tcttgtgaca tcgaagaccc
      361 gcgccaggct ccttttgaag ttttttctga atcccaacac cagcgcatat ctccgggggc
      421 ttgccgagga gttcggcgag tcaacgaacg gcgtacgcgt cgaactgaac cgcctctcgg
      481 aggcggggct tcttgaatgc gccgacgagg ggcgtaccaa ggtctaccgt gcgaatacgc
      541 gccaccctct ctttccggag ctgcagcgta tcgcggccaa gacgctcggg atcgaccagg
      601 ttgtggagca cgtcgtctcc cgcctcggca atgtggagct cgccctcgtg acgggcgact
      661 acgccaaggg gatcgattca ggacttatcg atctcgtgct tgttgggagg atcgacaaga
      721 tgtattttga gagccttgcg gagaagatcg aggggatcat gcagcggaag atacgccccc
      781 tcttcctcac gagagaggag tttttgcgtc tcaaggagcg catcgtttcc gagaacgcgc
      841 tgcttctgtg gggtgacgag ggcgagatcg cagcgaagta ggcgaacctt cgattccgcg
      901 gatttccggg aaagattgaa aagaaaaggg ggagacgagc atttccgcat cgtctccccc
      961 ttttctgtat tgcgaggcgt tctagaatcc cagctcttcg ccgacgatca gcacgtggaa
     1021 gttggtcccg agcgggcgtt tgctgatgat ggtgaggacg ttgcagatcc tggtcggtcc
     1081 ctggcagtcc atgcagacgc cggtccgcga acaggggttg ggagtgccca gccgcttgtt
     1141 gttgataggg gcggctttca tctggatgcg ctcctcggct gcggccagat ccttgacgat
     1201 cttgttcgtt cccgcgacga cgatgacctt gccggggccg aagatcatcg cggcgaccct
     1261 gtttccggag ccgtccatgt tgacgagtct gccgtccatg gtgagcgcgt tcgtgccgga
     1321 gaggaagacg tcgcagatct gctgcttcct gcggatatcg gccttctcct cgttggtcag
     1381 tccgggagcg ccgtggtcga ggatcgtgtt gccgcgctcg gcgagcttct cggatatccc
     1441 gatcgccttg agggtccacg aacccccgaa tccgacggat gcggcctcgg ggatcagttc
     1501 aagaaggcgc tgtgcggcgt ccgctccggt cgcgcagtac tcggcctcga agccgttctt
     1561 cttcagggct tcgacgacag ctccgccgac tgtttcattg tgccagggtg tgaattcact
     1621 cattgataga cctccttgtt ttgtttttct gaaaggagcg gggagcgaac tctccgttcc
     1681 ttctcaatat ccgtttattt tccctgtact ccggtaaggt accggagaat cccctcgccg
     1741 ggtcgaagca ggaccggcgt tccgggaagg actt
//
LOCUS       DAOTIL010000632         1771 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminobacteriaceae bacterium isolate AMR_MDS_3026
            NODE_79327_length_1771_cov_2.431235, whole genome shotgun sequence.
ACCESSION   DAOTIL010000632 DAOTIL010000000
VERSION     DAOTIL010000632.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868050
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminobacteriaceae bacterium (wastewater metagenome)
  ORGANISM  Aminobacteriaceae bacterium
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae.
REFERENCE   1  (bases 1 to 1771)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1771)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:19:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,224
            CDSs (total)                      :: 3,198
            Genes (coding)                    :: 3,184
            CDSs (with protein)               :: 3,184
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1771
                     /organism="Aminobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_79327_length_1771_cov_2.431235"
                     /isolate="AMR_MDS_3026"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:3034609"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(3..1259)
                     /locus_tag="P5201_11620"
     CDS             complement(3..1259)
                     /locus_tag="P5201_11620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013047895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRV99236.1"
                     /translation="MAFQEFRIDTHVFEEINDYLTDPKNPVLADLRRVVAKYGTVRDI
                     NERAREAGKVASLVKKLERMNSPFVKDLEWLASLRDEGKFVPLSDYRARVNPERAVWD
                     YDFSKAPTLEISALQYFPWLLAEVRQTVEKGEILPGRFIRVRKMKEQEADGGDLPAVR
                     AAVKILGATCVETLDTKGVDGSNVHLGGPGTITGYFGGIGQPNSHPLQWVDEVLYYYT
                     NYGVDEVLNINPGTVFLGYLLYRMGVDVRFKISVFMGNDNPYAVLWTLLAARMFARDD
                     GSTPLAGFNLSNSIDDAHIAKAAAIRRDLGLEDQVRIEHHITETWKSIVRQPYNRREG
                     LLKLAKTVPNISAKHEGGNPEDEQKLDHPSDILDYFREKKEVESSGEMDALTLNYLMK
                     HKAVNSTAEALTKAGLSFVAAGAHRL"
     gene            complement(1616..>1771)
                     /locus_tag="P5201_11625"
     CDS             complement(1616..>1771)
                     /locus_tag="P5201_11625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013047896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRV99237.1"
                     /translation="NALTFTTDTLRDVTIIGPGAGREETGQALLSDMLAIAAAERNSA
                     RFSVKAG"
BASE COUNT          278 a          534 c          586 g          373 t
ORIGIN      
        1 ctctacagcc ggtgcgcgcc cgccgcgacg aaggagaggc ccgccttcgt cagggcctcc
       61 gctgtgctgt tgacggcctt gtgcttcatc aggtagttca gcgtgagggc gtccatctcg
      121 ccggaggact cgacctcctt cttctcgcgg aagtagtcga ggatgtcgga cgggtggtcg
      181 agcttctgct cgtcctcggg gttgcctccc tcgtgcttgg cggagatgtt ggggacggtc
      241 ttcgccagct tcagcagccc ctccctgcgg ttgtacggct gccggacgat gctcttccac
      301 gtctcggtga tgtggtgctc gatgcgcacc tgatcctcca ggccgaggtc tctgcggatg
      361 gcggcggcct tcgcgatgtg cgcgtcgtct atggagttgg agaggttgaa cccggcgagc
      421 ggcgtgctcc cgtcgtcgcg ggcgaacatg cgggcggcga gcagcgtcca gaggacggcg
      481 taggggttgt cgttccccat gaaaacggag atcttgaacc gtacgtccac gcccatccgg
      541 tagagcaggt accccaggaa gaccgtgccg gggttgatgt tgagcacctc gtccactccg
      601 tagttcgtat aatagtagag cacctcgtcg acccactgga gcgggtggct gttcggctgg
      661 ccgataccgc cgaagtagcc ggtgatggtc cccggtccgc cgaggtggac gttggagccg
      721 tcgacgccct tggtgtccag cgtctcgacg caggtcgcgc ccagtatctt gaccgcggcg
      781 cggaccgcgg ggaggtcgcc cccgtcggcc tcctgctcct tcatcttgcg cacgcggatg
      841 aagcggcccg ggagaatctc ccccttttcc accgtctgcc ggacctcggc gagcagccac
      901 gggaagtact ggagcgcgct gatctcgagc gtcggagcct tggaaaagtc gtagtcccac
      961 acggcgcgct cggggttgac gcgggcgcgg tagtcggaga gggggacaaa cttcccctcg
     1021 tcgcggagcg aggccagcca ttcgaggtcc ttcacgaagg gggagttcat ccgctccagt
     1081 ttcttgacga gcgacgccac ctttccggct tcgcgggccc gttcgttgat gtcgcgtacc
     1141 gtgccgtatt tggcgacgac cctcctgagg tcggcgagca cggggttttt ggggtccgtc
     1201 agatagtcgt tgatctcctc gaaaacgtgc gtgtcgatgc ggaactcctg aaatgccatc
     1261 ggtgtttcct ccttataata gtgttgggtt acaaaaaacc gggggcctcc gaaatgttga
     1321 tttcgaagac ccccggcgga agcgtacgca gtgtgaggcg ccctacgctc cgggagggtc
     1381 gctgcacatc atcatggcga aaaagttaca gaagcgcgaa gcatgcgaag accgattcaa
     1441 taaagtcatg tcgccaagat tcctttctat ttcatggtat ggaacattct cctcgaaaac
     1501 gccgtttctg tcaaccgtgt tccggcgcat tgcgttcgtt cgccgtcgat ccgcagctag
     1561 ttcggcggat cgacggcgca ctgttctcgc gctgcgtccg cgtctgcggt tcgtcttacc
     1621 ccgccttgac cgagaagcgc gcgctgttcc gctcagcagc ggcgatggcg agcatgtcgg
     1681 agaggagggc ctgtccggtc tcctcgcgcc ccgcgccggg gccgatgatg gtgacgtcgc
     1741 gcagggtgtc ggtggtgaat gtgagcgcgt t
//
LOCUS       DAOTIL010000633         1770 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminobacteriaceae bacterium isolate AMR_MDS_3026
            NODE_79384_length_1770_cov_2.633236, whole genome shotgun sequence.
ACCESSION   DAOTIL010000633 DAOTIL010000000
VERSION     DAOTIL010000633.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868050
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminobacteriaceae bacterium (wastewater metagenome)
  ORGANISM  Aminobacteriaceae bacterium
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae.
REFERENCE   1  (bases 1 to 1770)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1770)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:19:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,224
            CDSs (total)                      :: 3,198
            Genes (coding)                    :: 3,184
            CDSs (with protein)               :: 3,184
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1770
                     /organism="Aminobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_79384_length_1770_cov_2.633236"
                     /isolate="AMR_MDS_3026"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:3034609"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..682
                     /locus_tag="P5201_11630"
     CDS             <1..682
                     /locus_tag="P5201_11630"
                     /inference="COORDINATES: protein motif:HMM:NF014910.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="FAD-linked oxidase C-terminal domain-containing
                     protein"
                     /protein_id="HRV99238.1"
                     /translation="IRTGIPVISCELLDKISVNITTKHVNTPLPYQDKADAFLLIQIE
                     GDSEERLEDGYEKVGNLCLQNGALEVFVAESRTESTAIWNIRQNLAEAMRAYDPYCSL
                     SGDMVVPLSAVPEMVEEIRKAAKERGIAVGILGHIADGNLHPIIFKPESMEPMEWAEY
                     AETFYDDLTSVALRLGGVGSGEHGIGYVKMPIFLHSKTDEELEIMRGIKRSFDPQGIL
                     NPGKLLGN"
     gene            762..>1770
                     /locus_tag="P5201_11635"
     CDS             762..>1770
                     /locus_tag="P5201_11635"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRV99239.1"
                     /translation="MIDKNRFGILAVAFVWFTTHFGGGFASGVQVVQYFVSYGWYALF
                     LPVLSIAIVTAVLYYAWTFAAIYCTFDYREWGNKYFKPFEALFSNAYEVMYILILLIA
                     SAVAFATGGTVMQQILGTPYVMNTVIVAVCIFFLTIFGADLVRKAASTMAVIIIAGML
                     LIYVGNLIVNFPKLLAVLKEASAPASFLDALWASLKYAGFQACAMGAYIAVADALKSR
                     EDAKKASIWGFIVNAGELWLATAGILLHYPAILTEKVPVLFVAAHGGGGAIGTAIVSL
                     LIFLAVVSTGVSLIFGGARRIVGLWGKVKGSTVAGDRKVNIAASLIYVLITWGIALFG
                     LI"
BASE COUNT          346 a          565 c          521 g          338 t
ORIGIN      
        1 catccgcacc ggcatcccgg tcatctcctg cgagctgctg gacaagatct cggtaaacat
       61 caccacgaag cacgtcaaca cgccgcttcc ctaccaggac aaagccgacg cctttttgct
      121 catccagata gaaggcgact cggaagagcg gcttgaggac gggtacgaga aggtgggaaa
      181 cctctgcctt cagaacggcg cgctggaggt cttcgtggcg gagagccgta ccgaatccac
      241 ggcaatatgg aacatccggc agaacctcgc cgaggctatg cgtgcctacg acccgtactg
      301 ctcgctcagc ggcgacatgg tggttcccct ctccgccgtc ccggagatgg tggaggagat
      361 acgcaaggcg gcgaaggaac gcggcatcgc ggtcggcatc ctgggacaca tcgccgacgg
      421 caacctccac ccgatcatct tcaagccgga gtcgatggag ccgatggagt gggcggagta
      481 cgcggagacc ttctacgacg atttgaccag cgtcgctctc cggctcggcg gcgtaggaag
      541 cggagagcac ggcatcggct acgtaaagat gcccatcttc cttcattcga agaccgacga
      601 agagctggag attatgcgcg ggatcaagcg atccttcgac ccgcagggca tcctgaaccc
      661 cggcaagctg ctgggaaact gacggcaaca gcgggggcgg cctgtacagg acacccccgg
      721 cgcactcata ccttactgta agcacgagag gagaagagat tatgatcgac aagaacaggt
      781 tcggcattct cgcagtggcg ttcgtctggt tcaccaccca cttcggcggc gggttcgcca
      841 gcggcgtgca ggtagtccag tatttcgtgt cctacgggtg gtacgcgctc ttcctgcccg
      901 tcctctccat cgccatcgtc actgcggtgc tctactacgc atggaccttc gccgctattt
      961 actgcacctt cgactaccgc gagtggggaa acaaatactt caaacccttc gaggcgctct
     1021 tctccaacgc ctatgaagta atgtacatcc tcatcctgct catcgcctcg gcggtggcct
     1081 tcgccaccgg cggaacggtg atgcagcaga tcctggggac tccctatgtc atgaacaccg
     1141 tgatcgtcgc cgtctgcatc ttcttcctca ccatcttcgg cgcggacctt gtcaggaagg
     1201 ccgcctccac aatggcggtg atcatcatcg ccggaatgct gctcatctac gtgggaaacc
     1261 tgatcgtcaa cttcccgaag ctgctcgcgg tcctcaagga ggcttccgcg cccgcgagct
     1321 tcctcgacgc gctctgggcg tcgctcaaat acgcgggctt ccaggcgtgc gcgatgggcg
     1381 cctacatcgc ggtggccgac gcgctgaaaa gccgagagga cgcgaagaag gcctcgatct
     1441 ggggcttcat cgtcaacgcg ggagagctct ggcttgcgac ggcgggcata ctcctccact
     1501 accccgccat ccttaccgag aaagttcccg tgctcttcgt ggccgcgcac ggcggcggcg
     1561 gcgcgatagg caccgccatc gtctcgctgc tcatcttcct cgccgtggtc tccaccggcg
     1621 tcagcctcat cttcggcggc gcgcggcgca tcgtgggcct gtgggggaaa gtaaagggat
     1681 cgacagtggc cggagacaga aaagtgaaca tcgcagcatc tctgatctac gtcctcatca
     1741 cgtggggcat cgcgctcttc ggtcttattc
//
LOCUS       DAOTIL010000634         1769 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminobacteriaceae bacterium isolate AMR_MDS_3026
            NODE_79450_length_1769_cov_2.804551, whole genome shotgun sequence.
ACCESSION   DAOTIL010000634 DAOTIL010000000
VERSION     DAOTIL010000634.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868050
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminobacteriaceae bacterium (wastewater metagenome)
  ORGANISM  Aminobacteriaceae bacterium
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae.
REFERENCE   1  (bases 1 to 1769)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1769)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:19:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,224
            CDSs (total)                      :: 3,198
            Genes (coding)                    :: 3,184
            CDSs (with protein)               :: 3,184
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1769
                     /organism="Aminobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_79450_length_1769_cov_2.804551"
                     /isolate="AMR_MDS_3026"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:3034609"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
BASE COUNT          507 a          417 c          355 g          490 t
ORIGIN      
        1 ggatttccca gactacattt tccgaatctg atttctcaaa acaagtgccc tgaacaggaa
       61 aaccggaggc cccttcgccg cggcaacacc cacgaaaagg aaaaaccctt tcgcttttca
      121 cgtcattcaa cttgattcag ttcgccccat gataaatcag aggaagagag cgtcccgacg
      181 caattattgg caatcttttt cttggaccgg aatatgtttt ttaaggggaa aattttttac
      241 taatacgttt tccaagtgct ttttacgcat gcgaatttat gtttgtggga gagagaacaa
      301 gaatgagatt ctaggctatc gaaatacgat ttccccggtg cgattaggca acagtgcaga
      361 tagtatatac tgtattatat atatacatat gcaacatata caggcgtttg ctcgcatcaa
      421 tcccgccgat cggatccgga aatcattttc cctcgaattt cattccacaa attctgttcc
      481 cccaaaaatc tttttcacag atgagatatt tccctgacac cgtcctcttg agcggacggt
      541 acaaggattt tcgaaaaatc ctgttcttga aaatcctatt cgtaaaacag accttccaaa
      601 aaaatttctc gctcaaatcc catttccctt atcgtatttt aaaaaactgt tccctaaaaa
      661 attctttcac tcaagcaacc tcaaaaaagt tgagttgccc tcgcagaaac ttgacgcaat
      721 cgagctcaat gaagagagaa acccatgcat tcagagcttc cgcaaatctt ctgcccctgt
      781 tctcgttcca aaaattgttc caggaaatct tttccggaga ttgcattcgc ttgcgatttc
      841 cccgagagac tgcggaaagg acacatctct tccgccccgt cctccatccg acaaccttcg
      901 ccctcgtaag agtgcgaagg ttgtattgaa tcgcaaaaca atcgtaaaaa agacggagcg
      961 caccgtacgt ccgcgacgaa tggagacgaa tggagttcaa ggatgttaaa gacagtggac
     1021 accgatctcg cacggaatag aatttcattt caatcgatta gcaaaatcca agaagctgtc
     1081 tttcgatggg ggaaagtctt gcataataga aatgacccca gaggaatttt cctcatgcaa
     1141 aaaaaaatct taagcagtcg ctccacaatc gaaacttcat ttccgcacat acaacccttc
     1201 agaatgcctc gtattgcatt ttgtgcataa aaacgacttc gagtacatct tagggcaatt
     1261 tcgatcagca ggaggcgagt ttgagggcaa atcaaagaag tatgaaacac agaacagcgt
     1321 ccggatatta aagctgggtg agtcaatctt ttctagtgga attttttcat ctgttggtcg
     1381 ggtattgcat ctactttctt tttcctcaag attcaaaagc aatcctcttc ctttttttaa
     1441 ctccgcatac attacgcttc ggcgtctctt aagagacgcc gaagcagtgc tgtcgggtca
     1501 tcccgtcccg gaagggacgg ggatgacgtt gagccctctt tagagggctc aaccaattta
     1561 accgggattt ttcgagggtt aaaatcttta ccagaatacg atttcctgaa gcaatttcac
     1621 tctggaaatc ttttcgtgga caggtggttt ttttctaccg aaatacgatt tctccggtgc
     1681 gttgccgcgg gagatggata ggcgggcagg cagtatatac aagcgttcgc tcgcaccgtt
     1741 cccaccatga atatagaatt aaatctttt
//
LOCUS       DAOTIL010000635         1766 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminobacteriaceae bacterium isolate AMR_MDS_3026
            NODE_79650_length_1766_cov_2.409117, whole genome shotgun sequence.
ACCESSION   DAOTIL010000635 DAOTIL010000000
VERSION     DAOTIL010000635.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868050
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminobacteriaceae bacterium (wastewater metagenome)
  ORGANISM  Aminobacteriaceae bacterium
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae.
REFERENCE   1  (bases 1 to 1766)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1766)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:19:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,224
            CDSs (total)                      :: 3,198
            Genes (coding)                    :: 3,184
            CDSs (with protein)               :: 3,184
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1766
                     /organism="Aminobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_79650_length_1766_cov_2.409117"
                     /isolate="AMR_MDS_3026"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:3034609"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..157
                     /locus_tag="P5201_11640"
     CDS             <1..157
                     /locus_tag="P5201_11640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005661208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="1-(5-phosphoribosyl)-5-amino-4-imidazole-
                     carboxylate carboxylase"
                     /protein_id="HRV99240.1"
                     /translation="VPTSTGYGANLGGIAPLLTMLNSCASGVAVMNIDNGLGAGFFAA
                     MVVRQSK"
     gene            complement(275..1507)
                     /gene="pepT"
                     /locus_tag="P5201_11645"
     CDS             complement(275..1507)
                     /gene="pepT"
                     /locus_tag="P5201_11645"
                     /EC_number="3.4.11.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009164794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0045148 - tripeptide aminopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase T"
                     /protein_id="HRV99241.1"
                     /translation="MPSVLDRFLKYIQIPSQAAHDAGKVPSTPGQMTLARELGEELKS
                     LGLADVVVDEHAYVTATLPGNTKGAPVIAFMAHLDTALEVTDDTVRPRLVENYDGGEI
                     ILNEADGVVLSPSTFPEMLLYKGETLVVTDGTTLLGADDKAGIAEIMAALEIMIARPE
                     LPRGDVKVAFAPDEEIGHGASLLDLKTFGADFAYTVDGGAVGEISYETFNAAKAKIVI
                     HGRSVHPGKSKDKMLNSILLGMELAAMFPANETPAHTEEYEGFFHLLGFTGNVETTEM
                     NYIIRDHSAEKFAERKKAVEAAVTAINKKYGEGRAELTMTDQYYNMLDKIKPQMHIVE
                     TAMEAMRRCGIEPKIIPVRGGTDGSQLSYRGLLTPNIFTGGHNAHGKHEFISVASMEK
                     AVAVVLKIVELYAGKSVR"
     gene            complement(1512..>1766)
                     /locus_tag="P5201_11650"
     CDS             complement(1512..>1766)
                     /locus_tag="P5201_11650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008521399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase"
                     /protein_id="HRV99242.1"
                     /translation="PYGFEFRKLLADLAAERSIPFRVETHFRYGSDASVAALAGFDAD
                     FACFGPGVDASHSYERTTIQAVKACCDLLTAYLVTDRKGV"
BASE COUNT          316 a          545 c          542 g          363 t
ORIGIN      
        1 cgtccccacg agtaccgggt acggagccaa cctcggcggg atcgcgccgc tgctcaccat
       61 gctcaactcg tgcgcctcgg gggtggcggt gatgaacatc gacaacgggc tcggagcggg
      121 attcttcgcg gcgatggtcg tccgacagtc gaagtaacac caaggctccc ctcggccgcg
      181 ggcatacgga gtacccgggg aaaaacggca gagcgaaagc aggggggagc gcattcagcg
      241 tctcccccct gcacgtaccg gcgaagtccg ctgtttaacg aacagatttc ccggcgtaga
      301 gttcgacgat cttcaggaca accgccacgg ccttctccat cgacgcgacg gagatgaatt
      361 cgtgcttccc gtgggcgttg tgccctccgg tgaagatatt cggcgtcaac agcccccggt
      421 aggagagctg cgaaccgtcc gtgccgccgc ggaccgggat gatcttcggc tcgattccgc
      481 agcgccgcat cgcctccatc gccgtctcga cgatgtgcat ctgcggcttg atcttgtcga
      541 gcatgttgta gtactggtcc gtcattgtca gctccgccct gccttcgccg tacttcttgt
      601 tgatggccgt cacggccgct tcgacggcct tcttgcgctc ggcgaatttc tccgcggagt
      661 gatcacggat gatatagttc atctccgtag tctcgacgtt tccggtgaat ccgagaaggt
      721 ggaagaaccc ctcgtactct tcggtgtgcg cgggcgtttc attggcgggg aacatggccg
      781 caagctccat gccgaggagg atggagttca gcatcttatc cttcgacttg ccggggtgga
      841 cgctccgtcc gtggatgacg atcttcgctt ttgccgcgtt gaaggtctcg tagctgatct
      901 cgccgaccgc gccgccgtcg acggtgtaag caaagtccgc gccgaacgtc ttcaggtcga
      961 gcaggctcgc gccgtgtccg atctcctcgt cgggagcgaa ggcaaccttg acgtcgcccc
     1021 tggggagttc cgggcgggcg atcataattt cgagggccgc cataatctct gcgatacccg
     1081 ccttgtcgtc ggcgccgagc agcgtcgtcc cgtcggtgac gacgagcgtc tcccccttgt
     1141 agagcagcat ttccgggaac gtcgacggcg acaggacaac gccatcggct tcgttcagga
     1201 tgatctctcc gccgtcgtag ttctctacga ggcggggacg cactgtgtcg tcggtcactt
     1261 cgagcgccgt atccaggtgc gccatgaacg caatgaccgg ggctcccttt gtgtttccgg
     1321 ggagggtcgc tgtcacatag gcgtgctcgt cgaccacgac gtcggcaagc cccagggact
     1381 tcagctcttc gccgagttcg cgggcaagcg tcatctgccc cggcgtgctc ggaaccttcc
     1441 cggcgtcatg cgccgcctgg ctgggaatct ggatatactt caaaaaacgg tcaagtactg
     1501 aaggcatagt tctaaactcc tttgcgatcc gtgacgaggt aggctgtaag aaggtcgcag
     1561 cacgccttca cggcttgtat tgttgtacgt tcgtacgaat gcgacgcgtc cactccgggg
     1621 ccgaaacagg caaagtccgc gtcaaaacct gcgagggccg caacgctcgc gtccgatccg
     1681 tagcggaaat gcgtttccac acggaaagga atggaccgct ccgcggcaag gtccgcaagt
     1741 aatttccgga actcgaaacc gtacgg
//
LOCUS       DAOTIL010000636         1765 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminobacteriaceae bacterium isolate AMR_MDS_3026
            NODE_79690_length_1765_cov_3.496491, whole genome shotgun sequence.
ACCESSION   DAOTIL010000636 DAOTIL010000000
VERSION     DAOTIL010000636.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868050
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminobacteriaceae bacterium (wastewater metagenome)
  ORGANISM  Aminobacteriaceae bacterium
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae.
REFERENCE   1  (bases 1 to 1765)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1765)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:19:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,224
            CDSs (total)                      :: 3,198
            Genes (coding)                    :: 3,184
            CDSs (with protein)               :: 3,184
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1765
                     /organism="Aminobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_79690_length_1765_cov_3.496491"
                     /isolate="AMR_MDS_3026"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:3034609"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..573
                     /locus_tag="P5201_11655"
     CDS             <1..573
                     /locus_tag="P5201_11655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015557192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HRV99243.1"
                     /translation="SWADIVKPEYAKNVRVANPGTSGTAYNMVTTIIRVFGNDEDKAF
                     DFLKQLDKSIDQYTRSGSAPGKSCAIGEIPVAIGYLHDLIKLKVENAPIEIALPSDGT
                     GFETAAMSLVKDGPDPVNAKKLYDWMLGQTAMDIIAGWYVIPLSSKASPIDTGYSFEK
                     MTLVDQNDVWDAANKARLLERWNKEIGSAR"
     gene            727..>1765
                     /locus_tag="P5201_11660"
     CDS             727..>1765
                     /locus_tag="P5201_11660"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HRV99244.1"
                     /translation="MNNALTGSFQSIAENHYRKSAVAAMGSVPSNDDAVSGWLRRIAP
                     SSGDFALIFLKGFPEDEEGMKVFAGAPELEAFFEMHRESDAFRKGIESSMYDEIFQPA
                     ERLQFAGEERRILFAPAHDGETGDVTGTAVFLFEPAGFDKFLVLMRGLFILALVIFTA
                     VSAVASYSRDPVLGYVILGLFAIVGVFVAYPLFEAVRLSFIQDGKFTLDTWKSILTAR
                     QYLTALQGSITLGVFTATASTIVGFLFAFVISRTGIGGKKFFSAMATLPIISPPFSLT
                     LSIILLFGNNGLITKRILALEGFSIYGLGGLTLVQTMGMFPIAFLTMTGVLEAIDSTL
                     EDAAMNLRATKW"
BASE COUNT          348 a          598 c          468 g          351 t
ORIGIN      
        1 tcctgggccg acatcgtcaa gcccgagtac gcgaagaacg tccgcgtcgc caacccaggc
       61 acctcgggca cggcgtacaa catggtgacg acgatcatcc gcgtcttcgg caacgacgag
      121 gacaaggcgt tcgacttcct caagcagctc gacaagagca tcgaccagta cacccgctcc
      181 ggctcggccc ccggcaagag ctgcgcgatc ggcgagatcc ccgtcgccat cggctacctg
      241 cacgatctga tcaagctcaa ggtcgagaac gcgcccatcg agatcgccct tccctcggac
      301 ggcaccggct tcgagactgc tgcgatgtcg cttgtgaagg acggtccgga cccagtcaac
      361 gcgaagaagc tctacgactg gatgctcggc cagaccgcca tggacatcat cgccggatgg
      421 tacgtcatcc cgctcagctc gaaagcctcc ccgatcgaca ccggctactc cttcgagaag
      481 atgacgctcg tcgaccagaa cgacgtctgg gacgccgcaa acaaggcacg tctgctggaa
      541 cgctggaaca aggagatcgg ctccgccagg tagcatccgc cgcactgtga aaatacgaag
      601 gggctcccaa gggggctcct tcgccttttc ttcaaacgca atggaaagga ggagaggaac
      661 attccttcga aaagatttct ctcgttgttt ctgtccgttc ttcttttcgc aacgctactt
      721 ttttggatga acaacgcgct tacaggctcc ttccagtcga tcgcggaaaa tcactaccgt
      781 aaatcggccg tcgccgcgat ggggtcggtg ccttcaaacg acgacgccgt ttccggttgg
      841 ctccgccgta ttgctccctc ttcgggcgat ttcgccctga tcttcctcaa gggcttcccc
      901 gaggacgagg agggaatgaa ggtcttcgca ggcgctcccg aactcgaagc attctttgaa
      961 atgcaccggg aaagcgacgc cttccgcaag gggatcgaaa gcagcatgta cgacgagatc
     1021 ttccagcccg ccgagcgcct ccagttcgcc ggcgaagagc ggcgcattct cttcgccccg
     1081 gcccacgacg gcgagacggg cgatgtgacc ggcacagcgg tctttctttt cgaacccgca
     1141 ggcttcgaca agtttctcgt cctcatgaga gggctcttca ttctggccct cgtcatcttc
     1201 accgctgtct ccgccgtggc aagttacagc cgcgaccccg ttctcgggta cgtcatcctc
     1261 ggcctcttcg ccatcgtcgg cgtcttcgtc gcctacccgc tcttcgaggc cgtccgactc
     1321 tccttcatcc aggacgggaa gttcacgctc gacacgtgga agtccatcct gaccgcacgg
     1381 cagtacctca ccgccttgca aggaagcatc acgctcggcg tcttcaccgc aacggcttcc
     1441 accatcgtcg gcttcctctt cgccttcgtc atctccagga ccgggatcgg cggaaagaag
     1501 ttcttctccg caatggcgac gctcccgatc atctcgcccc ccttctccct gacgctctcg
     1561 atcatccttc tcttcggcaa caacggcctt atcacaaaga ggatactcgc gctcgaagga
     1621 ttcagcatct acgggctcgg cggactgacg ctggtacaga ccatggggat gttccccatc
     1681 gcttttctca ccatgaccgg cgttctggaa gccatcgact ccaccctgga agacgcggcg
     1741 atgaacctcc gcgccaccaa gtggg
//
LOCUS       DAOTIL010000637         1765 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminobacteriaceae bacterium isolate AMR_MDS_3026
            NODE_79723_length_1765_cov_2.558480, whole genome shotgun sequence.
ACCESSION   DAOTIL010000637 DAOTIL010000000
VERSION     DAOTIL010000637.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868050
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminobacteriaceae bacterium (wastewater metagenome)
  ORGANISM  Aminobacteriaceae bacterium
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae.
REFERENCE   1  (bases 1 to 1765)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1765)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:19:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,224
            CDSs (total)                      :: 3,198
            Genes (coding)                    :: 3,184
            CDSs (with protein)               :: 3,184
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1765
                     /organism="Aminobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_79723_length_1765_cov_2.558480"
                     /isolate="AMR_MDS_3026"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:3034609"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..655
                     /locus_tag="P5201_11665"
     CDS             <1..655
                     /locus_tag="P5201_11665"
                     /inference="COORDINATES: protein motif:HMM:NF024703.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016818 - hydrolase activity, acting on
                     acid anhydrides, in phosphorus-containing anhydrides
                     [Evidence IEA];
                     GO_process: GO:0006139 - nucleobase-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="helicase C-terminal domain-containing protein"
                     /protein_id="HRV99245.1"
                     /translation="GGVLVLCSSLKRKSAVASALIRDVVGSGGILLEQGKMSRSSILT
                     TFASTKRSVLVASSSFREGFDAPGDNLTWVILDKLPFTRPDDPEFKRRARFLSDKGII
                     ENTFDFSVSELRKTLCQSVGRLIRTKSDWGAVTVVDSRALTRGLEWGLNEISFVPREK
                     WRVDSPPPDVWVLRMKSLKGGVCPKFEFGGDPVEDSDRREPYEILDMMRNSFGFTAD"
     gene            674..940
                     /locus_tag="P5201_11670"
     CDS             674..940
                     /locus_tag="P5201_11670"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRV99246.1"
                     /translation="MSETRFEFKKSALPDAGSVRCGEFMRDALPYEDGESVRVHPLEM
                     LLIEACVVGARLRSCEGGRFCFFPRYLPPRGDLMTPDQYISETR"
     gene            complement(1363..>1765)
                     /locus_tag="P5201_11675"
     CDS             complement(1363..>1765)
                     /locus_tag="P5201_11675"
                     /inference="COORDINATES: protein motif:HMM:NF024754.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="3'-5' exonuclease"
                     /protein_id="HRV99247.1"
                     /translation="KTLLKKHIPEYRYFREADLVTGDERIVVSTVHKAKGLQFENVVV
                     PECVEGVYPWWNAETDEARNEDARVLYVAMTRAMKRLILTRHTIFVNQWGKRFPKRRS
                     PFLECVGPCFRRMNLLPLSMERSERISGTLK"
BASE COUNT          371 a          430 c          532 g          432 t
ORIGIN      
        1 tggtggagtt cttgttcttt gttcgtcctt gaagaggaag tccgctgttg cgagcgctct
       61 cattcgcgat gtcgtgggct cgggagggat tctgcttgag cagggaaaga tgtctcgaag
      121 ttcgatattg acgacgttcg cgtcgacgaa gcgttctgtc ctggtggcga gttcttcgtt
      181 cagagagggt ttcgacgctc ccggtgataa tttgacgtgg gtaattctgg acaagttgcc
      241 gtttactcgt ccagacgatc cggagttcaa gcgcagggca cgatttcttt cggacaaggg
      301 aatcatagag aacacattcg atttttccgt ttcggagttg agaaagacgc tttgtcagtc
      361 cgtgggaagg ctgatacgga cgaagtcgga ctggggagcg gtgacggttg tcgattccag
      421 ggcgttgact cgtggtcttg agtggggttt gaatgagata tcgttcgtgc ctcgcgagaa
      481 gtggagggtt gactctccgc cgcccgacgt atgggtgctt cgtatgaagt cgttgaaggg
      541 cggagtatgc cccaagttcg aattcggtgg tgatcctgtg gaggattcgg ataggagaga
      601 gccgtacgaa atattggaca tgatgcgtaa ttcattcggt ttcaccgcgg attgaagaag
      661 ggagttattc gaaatgtccg agacaaggtt cgagttcaag aagagcgcgc ttcccgatgc
      721 gggatcggtc cgatgcggag agttcatgcg cgatgcgttg ccgtacgagg acggtgagtc
      781 ggtgcgcgtc catcctctcg aaatgcttct tatagaggcg tgtgtggtcg gtgcccgtct
      841 tcgttcctgc gagggaggcc gtttctgttt tttcccgcgg tatcttccgc ctcgcggtga
      901 tttgatgacg ccggaccagt acatctccga gacgcgctga cgacgcggta cagggaaaat
      961 ctgcggccgg gctcgctcgg accgagcggg acaaatagag cggagagtgt ttccttctcc
     1021 agcaacacca tgccggaagt acctgcttga gcggcgaaga aatggttgga gtggtcgctg
     1081 ccactggaag tcggggaaga aaacttttga aagccgctca ttccggagaa aaatgcgtcg
     1141 tctccgacgc cagactgcga gggcatacac ctggaactgg cgaagtcggg aatgacaatg
     1201 acgctccttt tggacgagta ttgcctaaag tgtcggggat ggagcgagat tccgtagcag
     1261 tacgcttggt tccgtaatat ttaccgaaag agatttgtca tcctctccac gtctaaaggc
     1321 gtggagagga tgacaaacgt aatcatttca aatccacgcg atttatttca aggtaccaga
     1381 gattcgctcc gaacgctcca tcgacaacgg aagaagattc atcctccgaa aacagggtcc
     1441 gacgcattcg aggaacgggc ttcgtctctt tgggaacctc ttgccccatt ggttgacgaa
     1501 gatcgtgtgc ctcgtcaaaa tcagccgctt catcgccctc gtcatggcga cgtacagcac
     1561 ccgcgcatcc tcgttcctgg cttcgtccgt ctccgcattc caccacgggt agacaccttc
     1621 gacgcactcg gggacgacga cgttctcgaa ctggagtccc ttcgccttgt ggacggtcga
     1681 aacgacgatc ctctcgtcgc ccgtcacaag atccgcctcc ctgaagtaac gatactctgg
     1741 aatgtgcttt ttaagcagcg ttttc
//
LOCUS       DAOTIL010000638         1765 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminobacteriaceae bacterium isolate AMR_MDS_3026
            NODE_79730_length_1765_cov_2.240936, whole genome shotgun sequence.
ACCESSION   DAOTIL010000638 DAOTIL010000000
VERSION     DAOTIL010000638.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868050
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminobacteriaceae bacterium (wastewater metagenome)
  ORGANISM  Aminobacteriaceae bacterium
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae.
REFERENCE   1  (bases 1 to 1765)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1765)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:19:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,224
            CDSs (total)                      :: 3,198
            Genes (coding)                    :: 3,184
            CDSs (with protein)               :: 3,184
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1765
                     /organism="Aminobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_79730_length_1765_cov_2.240936"
                     /isolate="AMR_MDS_3026"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:3034609"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..438
                     /locus_tag="P5201_11680"
     CDS             <1..438
                     /locus_tag="P5201_11680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013048921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter large permease subunit"
                     /protein_id="HRV99248.1"
                     /translation="IAMGQVFSWTVSFARIPNLILPPLLGDAPSTFRILSVLSIAYFI
                     GCMFVDPIVVIMILTPIFKPAVVASGLDNVLVGTIVTLQAAIGSTTPPFGCNIFTAIA
                     IFKQRYIDVVRGVFPFIAMMVLVSILIIIFPQIALFLRDLMIQ"
     gene            603..1658
                     /gene="dctP"
                     /locus_tag="P5201_11685"
     CDS             603..1658
                     /gene="dctP"
                     /locus_tag="P5201_11685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013048920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter substrate-binding protein DctP"
                     /protein_id="HRV99249.1"
                     /translation="MKKRMMRCASLTLVLVFLFVFAGGAAAAPKYQWRLACETIAGSI
                     ADLYAQEFSRLLKEKSNGDIQLDIFHSGTLGTPTEMFELTLNGAVEFCLTGPSQSSSI
                     VPENQIMGLQFLFSDDPIVNRDFFAQSKALNVMLNDIYREKGLSVLSYFTEGAMFWTA
                     NKAIRTPDDFKGVKIRVMPSELLVETYRAYGANPTPLSFTELYSGLQLNVVEGQENAP
                     ATIQEMKFMDVQKFLIGSRHNMYVMQHLVNVGFLDSLPEDVRKIVLDSVDEVRPYIHD
                     VQEELNKTRLQMMIDDFKSGQEYYDLTHEEREAFKEIAKKADAKYFELSRNPEFAKEL
                     LETFRAEMAELESKRGK"
BASE COUNT          404 a          474 c          484 g          403 t
ORIGIN      
        1 atcgcgatgg ggcaggtttt ttcatggacg gtctctttcg cccgaatccc gaacctgatc
       61 ctgccgccgc ttttgggcga tgcgccgtcc acttttcgga tattgtccgt tctgagcatt
      121 gcctacttca ttggctgtat gtttgttgat cccatagtcg tgatcatgat cttgacgccg
      181 atcttcaagc ctgcagtcgt cgcttccgga cttgataacg tgctcgtcgg gaccatcgta
      241 accctccaag cggccatcgg ttccacgacg cctccgttcg ggtgcaacat cttcaccgca
      301 atcgccatat tcaagcagcg atacatcgac gttgtacgcg gagtcttccc gtttatcgcg
      361 atgatggtgc ttgtctctat ccttatcatc atcttcccgc agatcgcgct cttcctcagg
      421 gatttgatga ttcagtaaga cgatagttac gctttaaggg attcaaccgt gtttgggggt
      481 aaataataaa ggggtgtttt taggggaagg aggaggaagt tcacggcgtt tcggggcaga
      541 aagaagagat tccggagagt gtttcggaca tgactttcga aagaaaaagg aggagttcta
      601 tcatgaaaaa gagaatgatg cgttgtgcga gtcttaccct tgtgctggtg ttcctgttcg
      661 tctttgccgg aggagctgcg gccgctccca agtatcagtg gcgtctggcc tgcgagacga
      721 tcgcggggag catcgccgat ctctatgcgc aggagttctc ccgcctgctg aaagagaaat
      781 cgaacggaga tattcagctt gacatcttcc actccggcac gctcggcacg ccgacggaga
      841 tgttcgaact gacgctcaac ggcgccgtcg aattctgtct gaccgggccg agccagtcca
      901 gctccatcgt tccggagaat cagatcatgg ggcttcagtt cctcttctcc gacgatccca
      961 tcgtgaacag agacttcttc gctcagagca aggcgctcaa cgttatgctc aacgacatct
     1021 accgggagaa ggggctctcc gttctctcct acttcaccga gggcgccatg ttctggacag
     1081 cgaacaaggc tatccgtacc cccgacgatt tcaagggcgt caagatccgc gttatgccct
     1141 ccgaactgct cgtggaaacc tacagagcgt acggagccaa cccgacgccg ctctccttca
     1201 ccgaactgta cagcggactt cagctcaacg tagtggaggg gcaggagaac gccccagcga
     1261 ccatccagga gatgaagttc atggatgtcc agaagttcct catcggttcg cgccataaca
     1321 tgtacgtcat gcagcatctt gtgaacgtcg gattcctcga ttcccttccc gaggatgtgc
     1381 gcaagatcgt cctcgactcg gttgacgagg tacgcccgta tattcacgac gttcaggaag
     1441 agctgaacaa gacccgcctg cagatgatga tcgacgactt caaatcggga caggagtact
     1501 acgacctgac ccacgaggag agagaagcgt tcaaggagat tgcgaagaaa gccgacgcga
     1561 agtacttcga gctttcgaga aatcccgagt tcgccaagga gctccttgaa acgttccggg
     1621 ccgaaatggc cgaactggag agcaaaagag gaaagtagcg gctgaaagcg gcagaggggg
     1681 gcttcggccc ccctcttcat gaagcattct atcgatgtgc aacgggtcat tgacaggagg
     1741 aacgcattat ggcaaagaaa cagta
//
LOCUS       DAOTIL010000639         1764 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminobacteriaceae bacterium isolate AMR_MDS_3026
            NODE_79759_length_1764_cov_3.559391, whole genome shotgun sequence.
ACCESSION   DAOTIL010000639 DAOTIL010000000
VERSION     DAOTIL010000639.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868050
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminobacteriaceae bacterium (wastewater metagenome)
  ORGANISM  Aminobacteriaceae bacterium
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae.
REFERENCE   1  (bases 1 to 1764)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1764)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:19:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,224
            CDSs (total)                      :: 3,198
            Genes (coding)                    :: 3,184
            CDSs (with protein)               :: 3,184
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1764
                     /organism="Aminobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_79759_length_1764_cov_3.559391"
                     /isolate="AMR_MDS_3026"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:3034609"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..316)
                     /locus_tag="P5201_11690"
     CDS             complement(<1..316)
                     /locus_tag="P5201_11690"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRV99250.1"
                     /translation="MKKYRILASFLILALCIVAAPASAKYMIVGNDEKMTWNEKGTVV
                     NLPSGKDTVQIFDISEGGTEPKLLVTLELMNSISGPPTNLAITKDEKLAILANPLECA
                     EKN"
     gene            complement(696..>1764)
                     /locus_tag="P5201_11695"
     CDS             complement(696..>1764)
                     /locus_tag="P5201_11695"
                     /EC_number="2.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013049074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="gamma-glutamyltransferase"
                     /protein_id="HRV99251.1"
                     /translation="ETFAPSGHAPRAGELWRSEAHARTLERIAGSNARDFYEGEIAAK
                     ILEAARKTGGYFSAEDLGSFVPEWVDPIGVNYKGYDVWEIPPNGQGIVALIALSILRG
                     FEFSSHDDPMTVHRQIEAIKMGFADAHRYVADTRFSGVPTAELLAAPYADARRALLGE
                     RAEARNAGNPLPGGTVYLCAADGEGNMISYIQSNYMGFGSGVVVPGAGVALNNRGHCF
                     SLEKGHPNMLEPGKRPYNTIIPSFLTKDGVPVGPFGVMGGFMQPQGHVQVVMNTVDFH
                     MNPQQALDAPRWQWMGGMNVSVEPGFSPALAQSLQRKGHAMKSELESNSFGRGEIIWR
                     TPEGTLGGATESRTDGCAATW"
BASE COUNT          334 a          542 c          524 g          364 t
ORIGIN      
        1 cgttcttctc agcgcattcc agagggttcg cgaggatggc gagcttctcg tcctttgtga
       61 tcgccaggtt cgtcggcggt ccggagatgg agttcatcag ttcaagagtg accagcagct
      121 tcggctcggt tccgccctcg gagatgtcga agatctgcac agtatccttt ccggaaggca
      181 gattcacgac ggttcctttt tcgttccacg tcatcttctc gtcgttgccc acgatcatgt
      241 acttcgccga agccggcgct gcgacgatgc acagcgcgag gatcaagaaa gacgcaagaa
      301 tccggtactt tttcattgag gcacgctcct tctataccaa accaacacta cgccgataca
      361 cgcgatgtcc cgaccttacc gatttgcgca tgcactcgct cattaccggt tccctatcac
      421 ctccgggggc tgcgaaacgc tttcggtcat cattagagaa ccccaaacgt ttcccgccga
      481 aaaaataacc tgattctaca acgcgatatt ttactgcaaa aaagtttatt ttactcccct
      541 tagacttccc aaaaacaaaa aatacatgtt cggaccttta cgttcccgga cgtgtttctc
      601 ttttcgagac aaacgtattc ggccgaacaa ctcgtaaaaa taaagccccc ccgatgctcc
      661 ggagaggctt cgcgcgtcgg ctgaaacgag agagattacc aggtggccgc gcagccgtcg
      721 gtgcgcgatt ccgtcgcgcc gccgagcgtt ccttcgggtg ttctccagat gatctcgccc
      781 ctgccgaagg agttggactc cagctcggac ttcatcgcgt gccctttgcg ctggaggctc
      841 tgcgcaaggg cgggagagaa gccgggttcg acggagacgt tcatcccgcc catccactgc
      901 cagcgggggg cgtcgagcgc ctgctgcggg ttcatgtgga agtcgacggt gttcatgaca
      961 acctgcacgt ggccctgcgg ctgcatgaag ccgcccatca cgccgaacgg ccccacgggg
     1021 acgccgtcct tcgtgaggaa ggagggaatg atggtgttgt acggccgctt gcccggttcg
     1081 agcatgttcg ggtgcccttt ttcgagcgag aagcagtgtc cgcggttgtt cagcgcaacg
     1141 ccggcgccgg ggaccacgac gccggagccg aagcccatgt agttgctctg gatgtaggag
     1201 atcatgttgc cctcgccgtc ggcggcgcag aggtagaccg tcccgccggg aagcgggttc
     1261 ccggcgttcc gcgcttcggc gcgttcgccc agaagagcgc ggcgcgcatc ggcgtacgga
     1321 gccgccagaa gctccgcggt cggaacgccg ctgaatcggg tgtccgcgac gtagcggtgc
     1381 gcgtcggcaa agcccatctt gatcgcttcg atctgccggt gaacggtcat agggtcgtcg
     1441 tgcgaggaga actcaaaccc gcgcagtatc gagagcgcga taagggcgac gattccctgt
     1501 ccgttggggg gaatctccca gacgtcgtac cccttgtagt tcacgccgat gggatcgacc
     1561 cattcaggaa caaaagaccc aaggtcctcg gcgctgaagt aaccaccggt tttccgtgcg
     1621 gcttcgagga tcttcgcggc gatctccccc tcgtagaagt cgcgcgcatt gctccccgcg
     1681 atccgctcca gcgtgcgggc gtgcgcctcc gatctccaga gctctccggc gcggggggcg
     1741 tgtcccgagg gagcgaaggt ctcg
//
LOCUS       DAOTIL010000640         1761 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminobacteriaceae bacterium isolate AMR_MDS_3026
            NODE_79934_length_1761_cov_5.099062, whole genome shotgun sequence.
ACCESSION   DAOTIL010000640 DAOTIL010000000
VERSION     DAOTIL010000640.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868050
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminobacteriaceae bacterium (wastewater metagenome)
  ORGANISM  Aminobacteriaceae bacterium
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae.
REFERENCE   1  (bases 1 to 1761)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1761)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:19:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,224
            CDSs (total)                      :: 3,198
            Genes (coding)                    :: 3,184
            CDSs (with protein)               :: 3,184
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1761
                     /organism="Aminobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_79934_length_1761_cov_5.099062"
                     /isolate="AMR_MDS_3026"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:3034609"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(85..1758)
                     /locus_tag="P5201_11700"
     CDS             complement(85..1758)
                     /locus_tag="P5201_11700"
                     /EC_number="6.3.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008519688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004329 - formate-tetrahydrofolate ligase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate--tetrahydrofolate ligase"
                     /protein_id="HRV99252.1"
                     /translation="MHVPSDIEIAQGSELRPIVEIAEKLGIGESELEQYGKYKAKISP
                     EVWDRVKGNADGKLILVTAITPTPAGEGKTTTTVGLAQALAKRGKKTSLAIREPSLGP
                     SFGVKGGAAGGGYSQVIPMEDINLHFTGDLHAITTAHNLISAMVDNHIQQGNELNLDP
                     RRIVWRRVMDLNERALRSVIVGLGGKANGVPRESGFDITVASEVMAILCLSTDLMDLK
                     TRIAKIVVGYTYDGKAVTVGEIGAAGSAAVLLKDAVKPNLVQTLEGVPAFIHGGPFAN
                     IAHGCNSIQATRLGLKLSDYFVTEAGFGADLGAEKFLDIKCRLGGLKPSAVVIVATVR
                     ALKMHGGRKKSELGEKDLVALEKGMTNLEKHIENIASFGLPAVVAINRFPADSEDELA
                     LIEKKCNALGASVALSEVWEKGGDGGLELADKVMEACGKKSEFVYQYEVGMSPKEKIE
                     RIAKNIYGASGVTYTDQAEKDLAQIHELGKDELLVCMAKTQYSLSDDPALLGRPSGFT
                     VTVREVRLSAGAGFLVAVTGSIMTMPGLPKKPAALSIDIDEKGRITGLF"
BASE COUNT          261 a          624 c          475 g          401 t
ORIGIN      
        1 caggacgact ttctccttga tcgccgccct gatctccgcc gacatctttt tcccgtcgag
       61 tatctttgcc gtcatggcag ccacctagaa gagccccgtg atgcggccct tttcgtcgat
      121 gtctatcgac agcgccgcag gcttcttcgg caatcccggc atcgtcatga tcgatcccgt
      181 caccgccacc aggaatccgg cccccgcaga cagtctcact tcgcgcaccg tcaccgtgaa
      241 tcccgaaggt ctccccagca gcgccgggtc gtcagagagc gagtactgcg tcttcgccat
      301 gcacaccagc aactcgtcct tcccaagctc gtgaatctgc gcgaggtcct tctccgcctg
      361 gtccgtgtac gtcactcccg acgctccgta gatattcttc gcgatccgct ctatcttctc
      421 cttcgggctc attcctactt cgtactggta gacgaactcc gacttcttcc cgcacgcttc
      481 catcactttg tccgcgagtt caaggccgcc gtctccgccc ttctcccaca cttccgacag
      541 ggctaccgac gcgccgagcg cgttgcattt cttctcgatg agcgctagct cgtcttcgct
      601 gtcggcggga aagcggttga tggcgacgac tgcgggaagg ccgaacgacg cgatgttctc
      661 tatgtgcttc tcgaggttgg tcattccctt ctccagcgcg acaaggtcct tctcgccgag
      721 ttcgctcttc ttcctcccgc cgtgcatctt cagcgcgcgc acggtggcga cgatgacgac
      781 cgcagaaggc ttgagtccgc cgagacggca cttgatgtcc aggaacttct ccgctccgag
      841 gtccgctccg aatccggctt ccgtgacgaa gtagtccgag agcttcaagc ccagccgcgt
      901 tgcctggatg gagttgcatc cgtgcgctat gttcgcgaag gggcctccgt ggatgaacgc
      961 gggcactcct tcgagcgtct gcacgaggtt cggtttcacg gcgtccttca gcagcaccgc
     1021 cgcggatccc gccgctccga tctctcccac ggtgacggcc ttgccgtcgt acgtgtagcc
     1081 gacgacgatc ttcgcgatcc gcgtcttcag gtccatgagg tcggtcgaca ggcacaggat
     1141 cgccatgacc tcagaggcca cggtgatgtc gaatccgctt tcgcgcggca cgccgttcgc
     1201 cttgcctccg agtccgacga tgacgctccg aagcgcccgt tcgttcaggt ccatgacgcg
     1261 gcgccatacg atccgccgcg ggtcgaggtt cagctcgttc ccctgctgga tgtggttgtc
     1321 caccatcgcc gagatcaggt tgtgcgccgt ggtgatcgcg tgaaggtcgc ccgtgaagtg
     1381 gaggttgatg tcctccatgg ggatgacctg gctgtaaccg cctccggcgg cccctccctt
     1441 gacgccgaag ctggggccca gggacggctc ccggatggcg aggctcgtct tcttcccgcg
     1501 cttcgccagc gcctgcgcaa gtccgaccgt cgtggtggtc ttcccttcgc ccgcgggcgt
     1561 cggcgtgatc gccgtcacca atatcagctt cccgtccgcg ttccccttca cccgatccca
     1621 tacttccggc gatattttcg ccttgtactt cccgtactgc tcaagctccg actctccgat
     1681 tcccagcttc tccgctatct ccactatcgg ccgcagttcc gatccctgcg ctatctctat
     1741 gtcgctcggc acatgcatta c
//
LOCUS       DAOTIL010000641         1761 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminobacteriaceae bacterium isolate AMR_MDS_3026
            NODE_79946_length_1761_cov_3.731536, whole genome shotgun sequence.
ACCESSION   DAOTIL010000641 DAOTIL010000000
VERSION     DAOTIL010000641.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868050
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminobacteriaceae bacterium (wastewater metagenome)
  ORGANISM  Aminobacteriaceae bacterium
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae.
REFERENCE   1  (bases 1 to 1761)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1761)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:19:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,224
            CDSs (total)                      :: 3,198
            Genes (coding)                    :: 3,184
            CDSs (with protein)               :: 3,184
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1761
                     /organism="Aminobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_79946_length_1761_cov_3.731536"
                     /isolate="AMR_MDS_3026"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:3034609"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..297
                     /locus_tag="P5201_11705"
     CDS             <1..297
                     /locus_tag="P5201_11705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013047807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HRV99253.1"
                     /translation="LGFLSLTGMLIKNAIVLIDQIDLEMREGKAPLDAIIDSGVSRTR
                     PVMMAALTTVLGMVPLYFDVLFSAMAVTIMFGLTFATALILVVVPVLYGEALKA"
     gene            complement(400..>1761)
                     /locus_tag="P5201_11710"
     CDS             complement(400..>1761)
                     /locus_tag="P5201_11710"
                     /inference="COORDINATES: protein motif:HMM:TIGR00797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042910 - xenobiotic transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006855 - xenobiotic transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HRV99254.1"
                     /translation="SMEKENTQYLKMIGMPVGRLILSLSVPTIVSMLVTAIYNIGDTY
                     FVSQLGTSASGAVGIVFSLMAIIQAVGFTVGMGSGAQISRLLGAKKVDEANTVAASGL
                     FAAIGLGSLVALFGLLFLDDLMRVLGATETILPYARDYASYILYAAPVMASSFLLNNI
                     LRAEGKAKFAMVGLTTGGLLNLALDPLFIFGFGWGIAGAAIATAISQCVSFGILFSWF
                     LRGKTIVRLSAASISRSPLEYLRILKNGFPSFCRQALASIATIALNVNAAVYGDAAVA
                     AMSIVGRLFMFVFVVNLGIGQGYMPVAGYNYGAGRFDRVKAAFLFTLKVTSAMMTLSG
                     IAAFMAAPSLIRIFIKTDPDVVRIGALALRAMCFAMPLIPLGVVCNMTFQSIGKSWTA
                     TVLSLARQGIFFLPLILVLPRFIGLTGVQITQPLADALTFLCCLPLAFFFFKELDAMS
                     KTN"
BASE COUNT          382 a          570 c          515 g          294 t
ORIGIN      
        1 ctgggcttcc tgagcctcac cggaatgctc atcaaaaacg ccatcgtcct gatcgaccag
       61 atcgacctgg agatgcggga agggaaggcc ccgctcgacg ccatcatcga ctcgggcgtc
      121 agccgcaccc gccccgtcat gatggccgcg ctcaccaccg tgttggggat ggtcccgctc
      181 tacttcgacg tcctcttctc ggcgatggcc gtcaccatca tgttcggcct caccttcgcc
      241 acggcgctca tcctcgtggt cgtcccggtg ctctacggag aagcgctgaa ggcgtagcgg
      301 caggcatact tatggaaaca acaacgcggc ggttcgcacc ttttcagcga accgccgcgt
      361 tgcgtcttcc cggacaatgt cgctccgatg cgccggtatt cagttcgtct tcgacatggc
      421 gtcgagctcc ttgaagaaaa agaacgcgag cggcagacag cagaggaagg tcagcgcatc
      481 ggcaagcggc tgcgtaatct gaacgccggt cagcccgatg aagcggggaa gaacgaggat
      541 cagcggcaaa aagaagatgc cctgccgggc gagtgacagc accgtcgccg tccacgactt
      601 cccgatggac tgaaaggtca tgttgcagac cacgccgagc ggaatcagcg gcatggcgaa
      661 acacatcgca cgcaacgcca gggcgccgat ccgcaccacg tcggggtcgg tcttgataaa
      721 gatcctgatc agggacggcg ccgccatgaa cgctgcaatt ccggagagcg tcatcatggc
      781 gctggtcact ttcagcgtga acagaaacgc agccttcacc ctgtcgaatc gtccggcgcc
      841 gtagttgtac cccgccacgg gcatgtaccc ctgcccgatc cccagattga ccacgaagac
      901 gaacatgaag agcctgccga cgatggacat cgcggccacg gcggcgtctc cgtacacggc
      961 ggcgttcacg ttgagcgcga tggtcgcgat gctggcgagc gcctgcctgc agaaggaggg
     1021 gaagccgttc ttgagtatac gaaggtactc taacggactg cgcgagatac tcgccgcgct
     1081 caaacgtacg atggtcttcc cccgaaggaa ccaggagaac aggatgccga agctgacgca
     1141 ctggctgatc gccgtcgcta tagcggcccc ggcgataccc cacccgaagc cgaagatgaa
     1201 cagggggtcg agcgccaggt tgagcagacc gccggtcgtc agcccgacca tggcgaactt
     1261 cgccttccct tccgcccgga ggatgttgtt cagcagaaag gacgacgcca taacgggcgc
     1321 ggcgtacaga atatagctcg cgtagtcccg ggcgtacggc aaaatcgtct ccgtcgcgcc
     1381 cagaacgcgc atcagatcgt cgagaaagag caaaccgaag agcgcgacca gcgacccaag
     1441 cccgatcgcc gcaaagagac cgcttgccgc caccgtattc gcttcgtcta ccttcttggc
     1501 gcccagcagc cgcgagatct gcgcgccgga acccatgccc acggtgaagc ccaccgcctg
     1561 gatgatcgcc atcagcgaaa agacgatgcc caccgcgccc gaagcgcttg tcccgagctg
     1621 cgagacaaag taggtatccc ctatgttgta gatcgccgtc accagcatgc tgacgatcgt
     1681 cgggaccgac agcgacagga tcagcctgcc caccggcatc cctatcatct tgagatactg
     1741 cgtattttct ttttccattg a
//
LOCUS       DAOTIL010000642         1759 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminobacteriaceae bacterium isolate AMR_MDS_3026
            NODE_80078_length_1759_cov_4.053404, whole genome shotgun sequence.
ACCESSION   DAOTIL010000642 DAOTIL010000000
VERSION     DAOTIL010000642.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868050
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminobacteriaceae bacterium (wastewater metagenome)
  ORGANISM  Aminobacteriaceae bacterium
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae.
REFERENCE   1  (bases 1 to 1759)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1759)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:19:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,224
            CDSs (total)                      :: 3,198
            Genes (coding)                    :: 3,184
            CDSs (with protein)               :: 3,184
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1759
                     /organism="Aminobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_80078_length_1759_cov_4.053404"
                     /isolate="AMR_MDS_3026"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:3034609"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(210..1721)
                     /locus_tag="P5201_11715"
     CDS             complement(210..1721)
                     /locus_tag="P5201_11715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005658634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0071705 - nitrogen compound transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BCCT family transporter"
                     /protein_id="HRV99255.1"
                     /translation="MQVKKDNSVFFISMAVTAATVLWGIFSPASFDAAAKGLFNVLIE
                     YFGWGYMLAMNIFVLFPILLCMSRFGKLRLGEPGSKPEFGNISWFAMLFSAGMGVGLV
                     FYGVGEPVYHFMTPPFGAAAGSAKAAEDALRTTFFHWGLHPWAGYAVIALCLAFFQYR
                     KGAPGLMSSMFLPVLGEKGNASMLGKTIDILAIFATIAGLATSLGLGTLQINSGLKYL
                     FNAPQNVTFQLVIIATLAFVYTGTAVTGIEKGIKFISNVNLFLACLLSVSLFFIGPTM
                     AIIESLMTGIGDYLSNVVNQSFHMAPYGGEYKGWLGGWTLYYWAWWIAWAPFVGSFIA
                     RISRGRTIREFVSGVLVVPALGSFTWFAIFGTSGLNLELTGAAKIAEKVAADISIGVF
                     EVYTHYPLGGIMSIIMVALISTFFITSANSATFVLSMYSSQGDMNPPKFRMGIWGVLM
                     AALAFVLLMSGGLQSLQIASIAAAAPFAVIMVIACWCLWKALKKDEATFTELN"
BASE COUNT          367 a          503 c          577 g          312 t
ORIGIN      
        1 gcaggggttt tcctcgtcga acaatagcag aaggcttcca tactccatgt gttcttcctc
       61 ctctcgaatc ttctcttttc gtacaaaaaa cgcccccgga tggcggagca tgccaggggc
      121 gcgtcgagac gcgtttttct cctctttctc ttcagccgta cgcattcccc ttcggaagcg
      181 tagcgcgaaa cggccggttc gtccagaggt cagttcagtt cggtgaaggt agcctcgtcc
      241 ttcttgagcg ccttccagag gcaccagcag gcgatcacca tgatcaccgc gaaaggcgcc
      301 gcagcggcga tggaggcgat ctgcagcgac tggagtccgc cgctcatcag caggacgaag
      361 gcgagcgccg ccatcaggac gccccagatg cccatgcgga acttcggcgg gttcatgtcg
      421 ccctggctgg agtacatcga gagaacgaac gtcgccgagt tggccgatgt gatgaagaag
      481 gtcgaaatca gcgccaccat gatgatcgac atgatgccgc ccagcgggta gtgggtgtag
      541 acctcgaaga cgccgatgga gatgtctgcg gcaaccttct ccgcgatctt cgccgcgccg
      601 gtgagttcga ggttcaggcc ggaggttccg aagatggcga accaggtgaa gctgccgagc
      661 gcgggcacga cgagcacgcc ggagacgaac tcacggatgg tgcggccgcg ggagatgcgg
      721 gcgatgaacg aaccgacgaa gggcgcccac gcgatccacc acgcccagta gtagagcgtc
      781 cagccgccga gccacccctt gtactcgccg ccgtagggcg ccatgtggaa gctctggttc
      841 accacgttgg agaggtagtc gccgatgccc gtcatcagcg attcgatgat cgccatcgtg
      901 ggcccgatga agaagagcga gacgctcagc aggcaggcga gaaagagatt gacgttggag
      961 atgaacttga ttcctttctc gatgccggtg actgccgtgc cggtgtacac aaacgcaagc
     1021 gtcgcgatga tgacgagctg gaaggtgacg ttctgcggcg cgttgaagag gtacttcagg
     1081 ccgctgttga tctggagcgt gcccaatccg agcgatgtgg caagcccggc gatggtggcg
     1141 aagatggcga gaatgtcgat ggtcttgccg agcatgctcg cattcccctt ttccccgagc
     1201 acggggagga acatgctgct catcagtccg ggagcgccct tgcggtactg gaagaaggcc
     1261 aggcagaggg cgatgacggc gtaccccgcc cacggatgga ggccccagtg gaagaaggtg
     1321 gtccgcagcg cgtcctcggc ggctttcgcg cttccggcgg ccgcgccgaa aggaggcgtc
     1381 atgaagtggt agacgggttc gccgacgccg tagaagacga ggccgacgcc catgcccgcg
     1441 gagaagagca tggcgaacca cgagatgttg ccgaattccg gcttcgaccc gggttcgccg
     1501 aggcggagct ttccgaagcg gctcatgcag aggagtatgg ggaagagcac gaagatgttc
     1561 atggcgagca tataccccca accgaagtac tcgatgagga cattgaaaag ccccttggcc
     1621 gccgcgtcga agctcgccgg ggagaagatc ccccagagca cggtggcggc tgtgacggcc
     1681 atggagatga agaacacgga attatctttt ttaacttgca tactcgtccc tccttcactg
     1741 atatccgggg gaggaggtc
//
LOCUS       DAOTIL010000643         1757 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminobacteriaceae bacterium isolate AMR_MDS_3026
            NODE_80221_length_1757_cov_2.876616, whole genome shotgun sequence.
ACCESSION   DAOTIL010000643 DAOTIL010000000
VERSION     DAOTIL010000643.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868050
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminobacteriaceae bacterium (wastewater metagenome)
  ORGANISM  Aminobacteriaceae bacterium
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae.
REFERENCE   1  (bases 1 to 1757)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1757)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:19:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,224
            CDSs (total)                      :: 3,198
            Genes (coding)                    :: 3,184
            CDSs (with protein)               :: 3,184
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1757
                     /organism="Aminobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_80221_length_1757_cov_2.876616"
                     /isolate="AMR_MDS_3026"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:3034609"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..990)
                     /locus_tag="P5201_11720"
     CDS             complement(<1..990)
                     /locus_tag="P5201_11720"
                     /inference="COORDINATES: protein motif:HMM:NF024816.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HRV99256.1"
                     /translation="MDMAKRWLDANLRKDPQLARRLIKQLLPEAERNGDVLGKAWLLF
                     QSGWLRLDADQYEQGCALLDSARSLFEAAGERAGVARCINALGRAYDRQGIFDLAIEL
                     YRESINLSEELGRSDIAGAASMNLAQSLCELGESGEALAVLEHCERHYSIAPHNRAVT
                     HYQKGSAYRALGRLEEAERILMDGVGMAAGASRDAMDMRCLLAETLMDRGRLDIALKL
                     ILKGLKDAAAFGERHVWIRFALAKTRFHLLQGSSDEAISVVQDARKAASELGARKLEA
                     EAERLLYKAYKAAGAHEEALAAFERYSEMKDLARNEQAASRIFGLHTERARREA"
     gene            complement(1109..1405)
                     /locus_tag="P5201_11725"
     CDS             complement(1109..1405)
                     /locus_tag="P5201_11725"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF015925.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004497 - monooxygenase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative quinol monooxygenase"
                     /protein_id="HRV99257.1"
                     /translation="MILVSAKFKAKPGMREKIVEISRYAVECTRKEKGNISYTLYTST
                     DDEETLMYFEEWTDIESLRAHLKTQHILDAREARKEMLAAPVEVKVFESKEVAL"
     gene            1473..>1757
                     /locus_tag="P5201_11730"
     CDS             1473..>1757
                     /locus_tag="P5201_11730"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRV99258.1"
                     /translation="MVIPLLKHHIIRCAASGDFLHTTSGRGDDGENQRAPRPIFGRGR
                     SLRITTESGAVPWLREETLRKRFYPAATGETLPGYASRPALRISMAFASSS"
BASE COUNT          347 a          568 c          440 g          402 t
ORIGIN      
        1 cgcctccctg cgggcgcgtt ccgtgtgcaa cccgaaaatt ctgctcgcgg cctgttcgtt
       61 acgcgccaga tccttcatct cggaatagcg ctcgaaagcg gccagggctt cttcgtgtgc
      121 tcctgcagcc ttgtacgcct tgtacagaag gcgttccgct tcggcttcca gtttgcgtgc
      181 tcccagttcc gacgccgctt ttctcgcatc ctgaacgaca gaaattgctt cgtcagagct
      241 cccttggagc aaatggaaac gggttttcgc gagtgcaaac ctgatccata cgtgccgttc
      301 gccgaaagca gcggcatcct tgagcccttt gagtataagc ttcagggcga tatcgagccg
      361 tcctctgtcc attaaggttt ccgccagaag acagcgcata tccatggcat cacgcgaagc
      421 tcccgcagcc atgcccaccc cgtccatcag gatgcgttcg gcctcctcca gcctccccag
      481 cgcgcggtac gcgctcccct tctgataatg cgtaacggcc ctgttatgag gggcgatgga
      541 ataatggcgc tcgcagtgtt ctaaaacagc gagcgcttcc ccggattcgc cgagttcgca
      601 gaggctctgc gccaggttca tagacgccgc cccggctata tccgaacgtc ccaactcttc
      661 ggacaggttg atcgactccc ggtataattc gatcgccaga tcaaaaattc cttgccggtc
      721 gtacgcgcgc cccagggcgt taatacatcg agccaccccg gcgcgctccc cggcggcttc
      781 gaacagagat cgagccgaat caagaagggc gcacccctgc tcgtactggt ccgcatccag
      841 acggagccac cccgactgaa aaagaagcca ggccttcccc aaaacatctc cgtttctctc
      901 tgcttcaggt aaaagttgtt taatcaatct acgagccagc tgcggatctt tgcgaaggtt
      961 cgcgtcaagc cagcgtttgg ccatatccat acgaccctcc ggagaagtcg ccttcgctag
     1021 cagagcatcc acatccgttg gctttccgct catgtccgtc acctcgcact cctcgcagct
     1081 gctgccatca tgacaaagct ccgctctctt agagcgcgac ctccttcgac tcgaagacct
     1141 tgacctcaac tggagctgcc agcatctcct tccgcgcttc gcgggcgtcg aggatatgct
     1201 gcgtcttcag gtgcgccctc aaggattcga tatccgtcca ctcctcgaaa tacatgagcg
     1261 tctcttcgtc gtcggtggac gtgtacagag tatagctgat gttccctttc tccttccgcg
     1321 tgcactctac agcgtaccgg ctgatttcga caatcttttc cctcattccg ggctttgctt
     1381 tgaacttcgc gctcacgagt atcatgtcga ttccaccttt cctggcatgt attctcacgg
     1441 aggcgcaacg gacgatttta ggagtctccg cgatggtaat accgttactg aagcatcata
     1501 tcatacggtg cgcagcttcc ggagattttt tacacactac ttcagggaga ggcgatgacg
     1561 gggaaaacca aagagcgccc cgccccatct tcgggcgagg acgctctctt cggataacaa
     1621 ctgaatccgg cgccgtcccg tggttacgag aggaaacgct gcgaaaacgc ttctaccctg
     1681 ccgcgacggg cgagactctg ccggggtatg cctccagacc tgccttgaga atatccatgg
     1741 ccttcgccag ctcttcg
//
LOCUS       DAOTIL010000644         1754 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminobacteriaceae bacterium isolate AMR_MDS_3026
            NODE_80395_length_1754_cov_3.672160, whole genome shotgun sequence.
ACCESSION   DAOTIL010000644 DAOTIL010000000
VERSION     DAOTIL010000644.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868050
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminobacteriaceae bacterium (wastewater metagenome)
  ORGANISM  Aminobacteriaceae bacterium
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae.
REFERENCE   1  (bases 1 to 1754)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1754)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:19:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,224
            CDSs (total)                      :: 3,198
            Genes (coding)                    :: 3,184
            CDSs (with protein)               :: 3,184
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1754
                     /organism="Aminobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_80395_length_1754_cov_3.672160"
                     /isolate="AMR_MDS_3026"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:3034609"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(243..995)
                     /locus_tag="P5201_11735"
     CDS             complement(243..995)
                     /locus_tag="P5201_11735"
                     /inference="COORDINATES: protein motif:HMM:NF015000.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase"
                     /protein_id="HRV99259.1"
                     /translation="MLVFGHRGASGYAPENTLPAFELGIRQGCEGFEFDVQLTKDGVA
                     VVHHDWTVDRTTTGSGAIPDLTFEEIRAFDAGKWFSEEFTGTTVPRLEELFELVPDTM
                     PLNVELKSRPHDRPGLEEAVAALLERYNRVENTIISSFNHKSLKTMRRLNPAFRLGIL
                     YEGVLLSPFSYAKRQDLDLYSLHPCHDYVSAELVEKAHARGIKVACWTVNTKERAREL
                     EAAGVDVIITNYPDRCLPEAPKGKKGKKKAGK"
     gene            complement(1063..>1754)
                     /locus_tag="P5201_11740"
     CDS             complement(1063..>1754)
                     /locus_tag="P5201_11740"
                     /inference="COORDINATES: protein motif:HMM:NF018513.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0031317 - tripartite ATP-independent
                     periplasmic transporter complex [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="TRAP transporter large permease"
                     /protein_id="HRV99260.1"
                     /translation="KPSRERRATAREMLRQVRESSWALFMPLGIILGLRFGMFTPTEA
                     GAIAVLYCSLVGFLVYRELRPVHFITILKDTVYGTSTVLLIIVAASVFGYYMTWERIP
                     QTVAALLLDIAHNKYTMLLVINIMLLILGMFIEGGAAMIILAPLLVPVVVGLGVDPVH
                     FGLVIIVNIMIGGVTPPFGSMMFTTCSITGVAIPDFIRESIPFIIALLIALLIVTYFP
                     ALTLFLPHLLY"
BASE COUNT          360 a          523 c          518 g          353 t
ORIGIN      
        1 aacttcaact ttacttttcg cttccgtccc cgctctttcc cgccgccgct cgggcggctc
       61 aaagagcgcg ccgtttcaaa gacgcgaggg gtataatacc acttttgcag aggttgtcaa
      121 cagcgttttt ttgaaaatcc gttttcttaa tagagagtct tcggccgtcc ctcccgctcc
      181 cattcaagac gcacgcgtcc tgcgctcccc gagagcgcag gacggagggg ctcggcctgt
      241 tgctacttac ccgccttttt cttccctttc ttccccttgg gcgcctccgg caggcacctg
      301 tcggggtagt tggtgatgat cacatctacg cccgcggcct ccagctcgcg ggcacgctcc
      361 ttcgtgttga ccgtccagca cgcgactttg atcccgcggg cgtgcgcctt ctccaccagc
      421 tccgccgaga catagtcgtg gcaggggtgc aggctgtaga gatccagatc ctggcgtttt
      481 gcgtaggaga acggggagag cagaacccct tcatagagta tccccaggcg aaacgcggga
      541 ttcagcctgc gcatcgtctt caggctttta tggttgaagg aggagatgat cgtgttctca
      601 acccggttgt accgctccag tagtgcggcg acggcttctt caagccctgg ccggtcgtgc
      661 ggtctgctct tcagctccac gttgagcggc atcgtatccg gaaccagctc gaacagctct
      721 tccaggcgcg gaacagtcgt ccctgtaaac tcctccgaga accatttccc cgcgtcgaac
      781 gcacgaattt cttcgaatgt gagatcgggt atggcgccgc tccccgtggt cgtgcgatcc
      841 acggtccagt catgatgaac gacggcaacg ccgtctttgg tcagctgcac atcgaattca
      901 aaaccctcgc acccctgccg gattccgagt tcaaaagccg gcagagtatt ctccggggcg
      961 taccccgagg cccccctgtg tccgaagaca agcactgaaa ccccttcttt ctataggtag
     1021 agagtatgta aggagcgcgc cgaagcgcgc tcctcccgtc gttcagtaga gcagatgcgg
     1081 taaaaagagc gtgagcgccg ggaaataggt cacgatcagc aacgctatga gcagtgcgat
     1141 gatgaacggg atgctctcac gtatgaagtc cggaatggcg acgccggtga tcgagcaggt
     1201 ggtgaacatc atcgacccga acgggggcgt cacgccgccg atcatgatgt tcacgatgat
     1261 gaccagtccg aaatgaacag gatccacacc gaggccgacg acgaccggca ccaggagcgg
     1321 cgccaggatg atcatggccg cgcccccctc gataaacatc ccgaggatga ggagcatgat
     1381 gttgatcacg aggagcatcg tgtacttgtt atgcgctatg tcgagcagca gcgcggcgac
     1441 ggtttgcggt atgcgctccc aggtcatgta gtagccgaag accgaggccg cgacgatgat
     1501 aaggagcacg gtgctggtac cgtagacggt atccttcaag atggtgatga aatgaaccgg
     1561 ccgaagctcg cggtagacga ggaaaccgac gagcgagcag tagagcaccg cgattgcgcc
     1621 ggcctcggtg ggggtaaaca tgccgaagcg cagtccgaga atgatgccga gcggcatgaa
     1681 gagcgcccag gaggactccc tgacctgccg cagcatctct cttgcagtgg cccgccgctc
     1741 acgcgagggt ttgt
//
LOCUS       DAOTIL010000645         1753 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminobacteriaceae bacterium isolate AMR_MDS_3026
            NODE_80470_length_1753_cov_2.923439, whole genome shotgun sequence.
ACCESSION   DAOTIL010000645 DAOTIL010000000
VERSION     DAOTIL010000645.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868050
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminobacteriaceae bacterium (wastewater metagenome)
  ORGANISM  Aminobacteriaceae bacterium
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae.
REFERENCE   1  (bases 1 to 1753)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1753)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:19:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,224
            CDSs (total)                      :: 3,198
            Genes (coding)                    :: 3,184
            CDSs (with protein)               :: 3,184
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1753
                     /organism="Aminobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_80470_length_1753_cov_2.923439"
                     /isolate="AMR_MDS_3026"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:3034609"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1113
                     /locus_tag="P5201_11745"
     CDS             <1..1113
                     /locus_tag="P5201_11745"
                     /inference="COORDINATES: protein motif:HMM:NF013740.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1905887 - autoinducer AI-2 transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HRV99261.1"
                     /translation="MGGKGVSSGEGQKTQMSRGWFLPFLFIFLALAYLSFTVSLPLLK
                     PLAWAVLLSFTVYPVFKRMRDRFFPRSITGNWAATIATGLIVLLLVIPSLFTGFVAAR
                     EGVRFYETVADVIAGMESSGESALTAFLPEQIVERMHPWMERYPLLRTGLQQAAGWAA
                     TTAVAASRAVLGSTFTFLYYLAVIIVVSFFLIRDGHLIIEYLKDIMPLPAKESAAFFV
                     RAKQVLQAVVYGVIVTALAQGLAGAVGWRIVGLPSPVFFGALMALFAMIPFLGTALVW
                     VPGALYLFYMSETTQALILLAWGVLVVSMVDSLLRPIFISGGGMVHLLIVFIGVIGGL
                     AAWGILGLFMGPLVVTLFVFLLESYRTMWKTFFVSR"
     gene            1186..>1753
                     /locus_tag="P5201_11750"
     CDS             1186..>1753
                     /locus_tag="P5201_11750"
                     /inference="COORDINATES: protein motif:HMM:NF015181.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-guanine dinucleotide biosynthesis
                     protein MobB"
                     /protein_id="HRV99262.1"
                     /translation="MPLLIAVSGFKDSGKTSLARALLTELSGRGLSLAFMKHTDRDVL
                     SPAGTDTGGIESQGIPCAYWGNDGLRMELPAAAPNSDTIAALFPEKDIVIVEGAKSIS
                     MPRIWVGSAESAPPEVKGVFACFDRAAEKGDGRFLYVPGEENELADRIAAFHERFANV
                     RCVRVAVRGKRLPLKPFVAEMIRGSIAGL"
BASE COUNT          285 a          492 c          566 g          410 t
ORIGIN      
        1 atgggaggga agggcgtttc ttccggagaa ggacagaaga cgcagatgtc gagagggtgg
       61 ttccttcctt ttctctttat cttccttgcg cttgcgtacc tctccttcac ggtctcgctt
      121 cctctgctca agccgctggc gtgggcggtg ctgctttcgt tcaccgtcta ccccgtcttc
      181 aaacggatgc gggaccgctt cttcccgcgt agtataacgg ggaactgggc cgctacgatt
      241 gcaacagggc ttatcgtcct gctgctcgtg attccctctc tttttacagg gttcgttgcg
      301 gcgcgggagg gcgtccgatt ctacgaaacc gtcgccgatg tgatcgcggg gatggagtcg
      361 tcgggcgaga gcgcgctgac cgcattcctg cccgagcaga tcgtcgagag aatgcacccg
      421 tggatggagc ggtatccgct gttgcgcacc ggattgcagc aggcggccgg atgggcggcg
      481 acaaccgccg tcgcggcatc gcgcgcggtt ttagggagta cctttacctt tctctactat
      541 ctcgccgtga tcatcgtggt gagtttcttt ctcatcaggg acggccacct tatcatcgag
      601 tacctcaagg acatcatgcc gcttccggcg aaggaaagcg ccgccttctt cgtccgcgcg
      661 aaacaggtgt tgcaagccgt ggtctacggc gtcatcgtca ccgctctggc tcaaggcttg
      721 gccggagccg tcggatggcg gatcgtcggt cttccgagtc cggtcttctt cggggctctg
      781 atggcgctct tcgcgatgat tccttttctg gggaccgctc tcgtttgggt tccgggggcg
      841 ctctatctct tctacatgtc ggagacgacg caggctctca tattgcttgc atggggcgtg
      901 ctcgtggtga gtatggtgga cagcctgctc cggccgattt tcatctccgg cggcgggatg
      961 gtacacctgc tcatcgtctt catcggcgtc atcggcggcc tagccgcatg ggggattctg
     1021 gggcttttta tggggccgct cgtggtgacg ctgttcgtct ttctgcttga gagctaccgg
     1081 acgatgtgga aaactttttt cgtctcccgg taacgccgcc ggaacgtgcc caatcatcaa
     1141 gaaggaggaa cgatgagtga acggacatat cgaaggaggc gggccatgcc tctcctgatc
     1201 gcagtctccg gtttcaagga ttcggggaag acgtcgcttg cgcgcgcgct tctcacggag
     1261 ctttccggac gggggctctc cctcgccttc atgaaacata cggacaggga cgtgctctcc
     1321 ccggccggca cggataccgg cggaatcgag tctcagggga taccgtgcgc ctactggggg
     1381 aacgacggcc tcaggatgga actgcctgca gccgctccga acagtgatac gatcgcggcg
     1441 ctctttccgg agaaggacat cgtcatcgtc gagggtgcga agagcatttc catgccgagg
     1501 atctgggttg gttccgcgga aagcgcgccc ccggaagtga agggcgtttt tgcgtgtttc
     1561 gacagggcgg ccgaaaaagg ggacgggcgt tttctctatg ttccgggcga ggagaatgaa
     1621 ctggcggatc gaattgcggc gttccatgag cgatttgcga acgtgcggtg cgtcagggtc
     1681 gccgttcgcg ggaagcggct tccgctcaaa ccctttgttg ccgagatgat ccgcggaagc
     1741 atcgcgggac tgt
//
LOCUS       DAOTIL010000646         1753 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminobacteriaceae bacterium isolate AMR_MDS_3026
            NODE_80484_length_1753_cov_2.531802, whole genome shotgun sequence.
ACCESSION   DAOTIL010000646 DAOTIL010000000
VERSION     DAOTIL010000646.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868050
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminobacteriaceae bacterium (wastewater metagenome)
  ORGANISM  Aminobacteriaceae bacterium
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae.
REFERENCE   1  (bases 1 to 1753)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1753)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:19:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,224
            CDSs (total)                      :: 3,198
            Genes (coding)                    :: 3,184
            CDSs (with protein)               :: 3,184
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1753
                     /organism="Aminobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_80484_length_1753_cov_2.531802"
                     /isolate="AMR_MDS_3026"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:3034609"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1228
                     /locus_tag="P5201_11755"
     CDS             <1..1228
                     /locus_tag="P5201_11755"
                     /EC_number="3.4.17.19"
                     /inference="COORDINATES: protein motif:HMM:NF014165.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004181 - metallocarboxypeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="carboxypeptidase M32"
                     /protein_id="HRV99263.1"
                     /translation="KAVKIPPDFLSAFYEHSARSYQVWTEARPANDFARVRPLLEKTL
                     DMSRRMADFFPGYDHIADPLIDFADYGMKTADVRKVFAELREALVPLVRAITSQEPAD
                     DSCLKKHFPEQQQLAFGRKVIERFGYDFSRGRQDKTHHPFETKFSTGDVRITTRIKED
                     DLGYGFFSTTHESGHALYEQGVNPSFEGTPLNEGTSSGVHESQSRTWENLVSRSRPFW
                     EHFYPELQATFPDQLGTVPLETFYRAVNTVRKSLIRTEADEVTYNLHVMIRFELELQM
                     LEGRLEVRDLPEAWHAAYEADLGITAPSDVDGCMQDVHWFGGHIGGAFQGYTLGNVMS
                     AQFFDAALAAHPEIPSETGRGEFGALHSWLKENIYRHGRKYTASELLHRVTGGGLEIG
                     PYIRYLRGKFGELYSL"
     gene            1234..>1753
                     /locus_tag="P5201_11760"
     CDS             1234..>1753
                     /locus_tag="P5201_11760"
                     /inference="COORDINATES: protein motif:HMM:NF014038.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class IV adenylate cyclase"
                     /protein_id="HRV99264.1"
                     /translation="MARNVEIKARAGNVAELTARVGRMATSVPEEIAQDDTFFRCPEG
                     RLKLRAFSDGAGQLIFYKRPDTEGPKTSFYSIAPVENADAMRETLAQALGQAGRVRKR
                     RTLFLVGRTRVHIDRVEDLGNFLELEVVLEEGEPEADGAREAEELLTALEIPQPALVD
                     RAYVDLLEAGGKG"
BASE COUNT          322 a          530 c          608 g          293 t
ORIGIN      
        1 gaaggcggtg aagataccgc ccgacttttt gtccgcattc tacgagcact ccgctaggag
       61 ctatcaggta tggacggagg cgcgccccgc gaacgacttc gcccgcgtgc ggccgttgct
      121 ggagaagacc ctcgacatga gccgccgcat ggcggacttc ttccccggat acgaccacat
      181 cgccgatccg ctgatcgact tcgccgacta cggtatgaag accgccgacg tgcggaaggt
      241 ctttgccgaa ctgcgcgaag cgcttgtgcc gctggtgcgg gcgatcacgt cgcaggagcc
      301 cgccgacgat tcctgcctca aaaagcactt tccggagcag cagcagctcg ccttcggacg
      361 gaaggtgatc gagcggttcg ggtacgactt ctcgcgcggc cgccaggaca agacgcatca
      421 tccgttcgag acgaagttct cgacgggcga cgtgcgcatc acgacgcgca tcaaggagga
      481 cgacctgggg tacgggttct tctccacgac gcacgagtcg gggcacgccc tctacgagca
      541 gggagtgaac ccttcgttcg aggggacgcc gctcaacgag ggaacgagct ccggcgtgca
      601 cgagtcgcag tcgcgcacct gggagaatct tgtctcacgc agccgcccct tctgggagca
      661 cttctacccg gaattacagg caaccttccc cgatcaactc ggaaccgtcc cgctggaaac
      721 cttctaccgc gctgtgaaca cggtacgaaa atcgctgatc cgcaccgagg ccgatgaggt
      781 aacgtacaac ctccacgtga tgatccgctt cgagctggag cttcaaatgc tcgaagggcg
      841 tctcgaagtc agggatcttc cggaggcgtg gcatgcggcg tacgaggcgg acctcggcat
      901 cacggcgccc agcgacgtcg acggatgcat gcaggacgtc cactggttcg gcgggcatat
      961 cggcggggcg ttccaggggt acacgctggg gaacgtgatg tcggcgcagt ttttcgacgc
     1021 ggcgctggcg gcgcacccgg agattccgtc ggagaccggt cgcggggagt tcggcgcgct
     1081 ccattcgtgg ctgaaggaga atatctaccg ccacggccgc aagtacacgg cgtccgagct
     1141 gctgcacagg gttaccggcg gcgggctgga gatcggcccg tacatccgct acctgcgcgg
     1201 gaagttcggc gagctgtact cgctctgagg acgatggcgc ggaacgtcga gatcaaggcg
     1261 cgggctggaa atgtcgcgga gctgacggcc agggtgggcc ggatggcgac gtccgtcccc
     1321 gaggagatcg cccaggacga tacgttcttc cgctgtccgg aggggaggct gaagctgcgc
     1381 gccttttcgg acggcgcggg gcagctcatc ttctacaagc gtccggatac ggaggggccg
     1441 aagacgtcgt tctacagcat cgctcccgtg gagaatgccg acgcgatgcg ggagactctt
     1501 gcgcaagctt tgggacaggc ggggcgggtg cgcaaacgac ggacgctctt cctggtcggg
     1561 cggacgcgcg tgcatatcga ccgcgtggag gatttgggca acttccttga gctggaggtg
     1621 gtcctcgaag agggggagcc ggaggccgac ggagcccgcg aggcggaaga gcttctgacc
     1681 gcgctggaga ttccgcagcc ggccctcgtc gaccgtgcgt acgtcgatct gctggaggcc
     1741 ggcgggaagg gtg
//
LOCUS       DAOTIL010000647         1752 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminobacteriaceae bacterium isolate AMR_MDS_3026
            NODE_80539_length_1752_cov_3.005893, whole genome shotgun sequence.
ACCESSION   DAOTIL010000647 DAOTIL010000000
VERSION     DAOTIL010000647.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868050
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminobacteriaceae bacterium (wastewater metagenome)
  ORGANISM  Aminobacteriaceae bacterium
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae.
REFERENCE   1  (bases 1 to 1752)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1752)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:19:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,224
            CDSs (total)                      :: 3,198
            Genes (coding)                    :: 3,184
            CDSs (with protein)               :: 3,184
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1752
                     /organism="Aminobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_80539_length_1752_cov_3.005893"
                     /isolate="AMR_MDS_3026"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:3034609"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..297)
                     /locus_tag="P5201_11765"
     CDS             complement(<1..297)
                     /locus_tag="P5201_11765"
                     /inference="COORDINATES: protein motif:HMM:NF023807.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-seryl-tRNA(Sec) selenium transferase"
                     /protein_id="HRV99265.1"
                     /translation="MNPNEKQTGAFRSLPSMDVLLGSPCVEPFFSMIGRESVKSILTE
                     ALQSERKELLNGRSDHASCESVLARAKPLLERRGAKSLIPVINATGVVVHTNLGR"
     gene            464..1132
                     /gene="yedF"
                     /locus_tag="P5201_11770"
     CDS             464..1132
                     /gene="yedF"
                     /locus_tag="P5201_11770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015555945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase-like selenium metabolism
                     protein YedF"
                     /protein_id="HRV99266.1"
                     /translation="MGKIDARGRSCPQPVMMTKAHVEKGEDEIEVFLDNAISASNVQR
                     YLEKNGYFVRIFDEDGSIIIRASVITEASLPADNAPLTQTTDGAALNTPPLPKTVTVS
                     AAPAAARSTASTAVLITKKGIGGDDSLLGEVLMKGFLGTISQMAPLPSVLALMNEGVK
                     LAMKHTSSLDHLMLLEKKGVRILVCGTCTNHFGVTSDIGAGVISNMFEITEALLASDK
                     ILSL"
     gene            complement(1198..>1752)
                     /locus_tag="P5201_11775"
     CDS             complement(1198..>1752)
                     /locus_tag="P5201_11775"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRV99267.1"
                     /translation="DSYVVEDILIVLSRFFPARPVKGYSVEVADSLRGGLSFIEGDGD
                     TDSLGKGLLLSLARVYVSMDEEGGSVLIAADSMEERARWISSQWKSRSGKNFNGVIHH
                     TYQGNGWSLVGIAAPGWMVLFDDETENAEQDWLEADLRVAYLPPDDEVLDFLSPEKTS
                     AEVLSLLAEYLLFCLPSREESPGI"
BASE COUNT          431 a          453 c          454 g          414 t
ORIGIN      
        1 acggccgagg ttcgtatgaa cgacgactcc tgtagcattt attacgggga ttaaactttt
       61 cgctcctcgt cgttccaaaa ggggtttcgc gcgcgcgagc actgactcgc aggaggcgtg
      121 gtcagaacgt ccgttgagta attcttttct ttcggactgg agcgcttctg tgagtatgga
      181 ctttacggac tcgcgcccga tcatcgaaaa gaaaggttcc acgcacggcg aaccaagaag
      241 aacgtccatt gaaggaagcg agcgaaacgc ccctgtttgt ttttcgttcg gattcacgag
      301 agatcattca cctttccatc gtcccgtagt gtggacgaac agctatttca aatataattt
      361 gtccttgttt ctattctaca ttataattgg tccgacggag agcaacactg tgtcattatt
      421 tcctccgcgc tttctaaaaa gcgcggggta gggaggcagt agaatgggga agatagacgc
      481 acggggcagg agctgcccgc aaccggtcat gatgacgaag gcgcatgtag aaaaagggga
      541 agatgagatc gaagttttcc tcgacaatgc gatttccgca tccaatgtac agcgatatct
      601 agaaaaaaac ggctacttcg tccggatttt cgatgaggac ggatcgatca ttattcgcgc
      661 ttccgtgatt accgaagcct cgctgccggc cgacaacgca ccattaacgc aaacgacgga
      721 tggcgctgcg ctgaatactc ctccgcttcc gaagaccgta actgtctccg ctgcaccggc
      781 ggcggctcgt tctacagcat cgacagctgt actcatcaca aagaaaggaa taggaggaga
      841 cgattccctc ttgggggagg ttcttatgaa ggggttcctt ggaacgatat cccagatggc
      901 tccgcttccc tcggttctcg ccctgatgaa cgaaggcgta aagcttgcga tgaagcatac
      961 ttcttctttg gatcatctga tgcttctgga gaaaaaaggc gtccgcattc ttgtctgcgg
     1021 aacgtgtacg aaccactttg gcgttacatc ggacatcggc gccggggtga tctccaatat
     1081 gttcgaaatc accgaagcgc ttctcgcttc ggacaagatt ctttctttgt aggatactgc
     1141 tcaataggcg ctcctcgtgt cgttatcgaa gagcgcctat tgttttttga aagatggtta
     1201 tattcccggg gattcttctc tcgaaggaag acaaaagagg agatattcgg caagaaggga
     1261 gagaacttca gcagaggttt tttcggggct gagaaaatcg agcacctcgt cgtcaggggg
     1321 aaggtacgca acgcgaagat cggcctccaa ccagtcctgt tcagcatttt cagtttcatc
     1381 atcaaagagc accatccatc ccggcgctgc gattcccacc aggctccatc cgttcccctg
     1441 ataggtatgg tgaatgacgc cgttgaaatt tttccctgag cgtgatttcc actgcgagct
     1501 gatccagcga gcccgttctt ccattgagtc ggcggcgatc aaaacgctcc ccccctcttc
     1561 gtccattgag acatagacgc gagcaagtga aaggagaagc cccttgccaa gggagtctgt
     1621 atccccgtcc ccctcaataa aagagagccc tccgcgcagc gaatcagcca cctcaacgga
     1681 ataccccttc acagggcggg cggggaaaaa gcgcgagagt acaatcagaa tatcctctac
     1741 gacataagaa tc
//
LOCUS       DAOTIL010000648         1749 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminobacteriaceae bacterium isolate AMR_MDS_3026
            NODE_80724_length_1749_cov_3.333530, whole genome shotgun sequence.
ACCESSION   DAOTIL010000648 DAOTIL010000000
VERSION     DAOTIL010000648.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868050
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminobacteriaceae bacterium (wastewater metagenome)
  ORGANISM  Aminobacteriaceae bacterium
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae.
REFERENCE   1  (bases 1 to 1749)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1749)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:19:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,224
            CDSs (total)                      :: 3,198
            Genes (coding)                    :: 3,184
            CDSs (with protein)               :: 3,184
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1749
                     /organism="Aminobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_80724_length_1749_cov_3.333530"
                     /isolate="AMR_MDS_3026"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:3034609"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..756
                     /locus_tag="P5201_11780"
     CDS             <1..756
                     /locus_tag="P5201_11780"
                     /inference="COORDINATES: protein motif:HMM:NF013502.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CheB methylesterase domain-containing protein"
                     /protein_id="HRV99268.1"
                     /translation="EAMDAGAVAILPKPADISAGEDGYSAELLKTVNEAAVSKVGRLR
                     QRKRPMKSDDAPRNPLQSSERRISLVGVGASTGGPQALAEFLARLPADFPRPLLVVQH
                     IAAGFTSGLAEWLGKVTPLPVRVAQNGQKAMNGVVHIAPDGFHMGVRQGGFLSLAKDP
                     PEHMVRPSASVLFRSLAAAYGGAAAVILFSGMGKDGAAEMKTLRNMGAATFAQDRESS
                     VVWGMPGEAVRLDAAEHVGSPAAIASLLLDQFR"
     gene            849..>1749
                     /locus_tag="P5201_11785"
     CDS             849..>1749
                     /locus_tag="P5201_11785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005659765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HRV99269.1"
                     /translation="MLSRIERADVMKEHAEILIVEDSLTQAKRLERLLVQNGYVVYVA
                     RNGAEGLEAAARKKPTVVISDVMMPEMDGYEMCRRIKNTEEIKGTPVVLLTSLSDPRD
                     VIKGLECGADNFLTKPYDGEHLIRRLEHILTNLELRKAGQAQMTVEVFFAGQYHRLTA
                     DRIQIIDLLLSTFEAAVLQSTQLEKLGGDYRNALEDVKRAQANFQTLMETTGDAVIVA
                     GDNGTIYYANPAAENLFDTKASDLVGRPFAFPLDVKGEEHREITISRSDGSKVVGDMR
                     VATSNWDGELVQFATIRDISEMVH"
BASE COUNT          377 a          468 c          548 g          356 t
ORIGIN      
        1 gaggctatgg atgcaggcgc cgtggctatt ctccccaagc ccgcggatat ctccgccggg
       61 gaagacgggt actctgcgga gcttttgaaa actgtaaacg aggctgccgt atcaaaagta
      121 ggacgtctgc ggcagagaaa aaggccgatg aagagcgatg atgctccccg gaatcctctc
      181 cagtcctctg aacgacggat atcgcttgtc ggcgtcggcg cctcgacagg aggtccgcag
      241 gctcttgcgg agtttcttgc gcggcttccg gcggatttcc cgcgccctct cctcgtcgtg
      301 cagcatatag ccgccgggtt tacctcggga ttggcggagt ggcttggaaa agtgacgcct
      361 cttccggtac gtgtcgccca aaatggacag aaggcgatga acggcgtcgt ccatattgct
      421 cccgacggtt ttcatatggg agttcgccag ggaggttttc tttctcttgc aaaagatccg
      481 ccggagcata tggttcgtcc gtccgcgtca gtgctcttcc ggagcctcgc cgcggcgtat
      541 gggggagccg cggccgtcat cctcttctcc ggaatgggaa aggacggcgc cgcggaaatg
      601 aaaacgcttc ggaatatggg ggccgccacg ttcgcccagg acagagagag ttccgttgtc
      661 tggggcatgc ccggggaggc tgtgcgcctg gatgcagcgg aacatgtagg atctcccgcg
      721 gcgatcgcct cccttcttct ggatcagttc cgttaaactg agaccggtgg cgcgcatgat
      781 tctttggcga gagtgaaacg cacttcattc gggaggaaaa tataccgaaa atatggaggg
      841 accactccat gttgagcagg attgaaaggg ctgacgtgat gaaagaacat gctgagatcc
      901 tcattgtcga ggacagtctg acccaggcga agcggttgga gcgtctcctt gtgcagaacg
      961 gctacgttgt gtacgtagcc agaaacggcg ccgaggggtt ggaagccgcc gccaggaaaa
     1021 agcccacggt ggtgatctcg gacgtcatga tgcccgagat ggacgggtac gagatgtgca
     1081 ggcgcataaa gaacaccgag gaaatcaagg ggacgccggt ggtactgctc acctcgctct
     1141 ccgatccgag ggacgtgatc aaagggcttg aatgcggcgc tgacaatttt cttaccaaac
     1201 cgtacgacgg cgagcatctg atccggcgtc tcgaacatat actgaccaat ttggagctga
     1261 gaaaagccgg gcaggcgcag atgacggtcg aggttttttt cgcagggcag tatcatcggc
     1321 tcactgcgga ccggatacag atcatcgacc tgttgctctc cacattcgag gcggccgtgc
     1381 tgcagagcac gcagcttgaa aagctcggcg gggactaccg gaatgccctc gaagacgtga
     1441 aacgggccca ggccaacttt cagacgctga tggaaactac cggcgacgcc gtgattgtgg
     1501 cgggcgataa cggcactatc tactacgcta accccgccgc cgagaatctt tttgacacca
     1561 aggcgtccga cctggtagga cgtccttttg ccttcccgct cgacgtcaag ggggaagaac
     1621 acagggagat cacaatttca cggtcggacg ggtcgaaggt cgtgggcgat atgcgcgtgg
     1681 cgacctccaa ctgggacgga gagcttgttc aatttgctac cattcgtgat atttccgaga
     1741 tggtccatc
//
LOCUS       DAOTIL010000649         1747 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminobacteriaceae bacterium isolate AMR_MDS_3026
            NODE_80849_length_1747_cov_4.367612, whole genome shotgun sequence.
ACCESSION   DAOTIL010000649 DAOTIL010000000
VERSION     DAOTIL010000649.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868050
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminobacteriaceae bacterium (wastewater metagenome)
  ORGANISM  Aminobacteriaceae bacterium
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae.
REFERENCE   1  (bases 1 to 1747)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1747)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:19:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,224
            CDSs (total)                      :: 3,198
            Genes (coding)                    :: 3,184
            CDSs (with protein)               :: 3,184
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1747
                     /organism="Aminobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_80849_length_1747_cov_4.367612"
                     /isolate="AMR_MDS_3026"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:3034609"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..692
                     /locus_tag="P5201_11790"
     CDS             <1..692
                     /locus_tag="P5201_11790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006583323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016625 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, iron-sulfur
                     protein as acceptor [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="aldehyde ferredoxin oxidoreductase C-terminal
                     domain-containing protein"
                     /protein_id="HRV99270.1"
                     /translation="QESVAQWGGYFLTLKGPQHDEAWVIFMDMVNKQLPTFEDKAEAL
                     FYFPNFRLWFSLTGVCKLPWNDVEPSDNRIRYKGIEAAKVPEHVQNYLDVYTAVTGKP
                     LSKEEMILQSERVYNFERVFNLRMGKGTRADHNIPDRALGPVFEDEWNARPEYFDGKL
                     SEAGISPEGLPLKEKMTKLLEFRRSEWEKLVDAVYKRRGWNANGVPTLETLKRLGIDF
                     PEVVDVVKAHQ"
     gene            720..917
                     /gene="thiS"
                     /locus_tag="P5201_11795"
     CDS             720..917
                     /gene="thiS"
                     /locus_tag="P5201_11795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009200507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfur carrier protein ThiS"
                     /protein_id="HRV99271.1"
                     /translation="MIRVNGREMEWRRGMTIQDVLDECGYTFRMIAVWIDGTPYRREH
                     FATAVVPDRAEVQALHMISGG"
     gene            926..>1747
                     /locus_tag="P5201_11800"
     CDS             926..>1747
                     /locus_tag="P5201_11800"
                     /inference="COORDINATES: protein motif:HMM:TIGR02494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0043687 - post-translational protein
                     modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycyl-radical enzyme activating protein"
                     /protein_id="HRV99272.1"
                     /translation="MLFGKEPTGFIFDVKRYSIHDGPGIRTTFFFKGCPLSCWWCHNP
                     EGISPLPAPTYHGNRCIACGRCVRECPNGARSFGPEGIALDSSKCVECRICEQVCPTR
                     ALEFVGKEMTVEEIVRLAKKDLAFYDESGGGVTFSGGEPLMQAEFLIACLEALRSLEI
                     STAVDTSGFCREEDLLLAAEEADIFLFDIKLIDPKLHEYYTGVGNERILANLRSLDKK
                     LVEQGRGSINIRIPLIPGVNNSPENLEAVIACVLSLRSVSGVNILPYHSIGSGKYK"
BASE COUNT          423 a          444 c          483 g          397 t
ORIGIN      
        1 gccaggagtc ggttgcccaa tggggcggtt atttccttac gctcaagggg cctcagcatg
       61 acgaagcatg ggttatcttc atggacatgg tgaataaaca gctcccaacc tttgaagata
      121 aagcggaagc gctcttctac ttccccaact tccgtctgtg gttctcgctc accggcgtct
      181 gcaagctccc ctggaacgac gtggaacctt cggacaaccg aatccgctac aagggtatcg
      241 aggccgccaa ggtcccggag cacgttcaga actatcttga cgtctatacc gccgtaaccg
      301 gcaagccgct gagcaaagag gagatgatcc tccagtcgga gcgtgtctac aatttcgaaa
      361 gggttttcaa cctccgcatg gggaagggaa cgcgcgcgga ccataacatt ccggaccgcg
      421 ccctgggccc cgtctttgaa gacgagtgga acgcgcgtcc tgagtacttc gacggcaagc
      481 tcagtgaagc gggtatttcc cccgaaggtc tgcctttgaa agaaaagatg acaaaacttc
      541 tcgaattcag acgaagcgaa tgggaaaaac tcgtcgacgc cgtctacaag cgcagagggt
      601 ggaatgcgaa cggagttccg acactggaga cactcaaacg ccttggcatc gacttccccg
      661 aagtcgtcga cgtcgtcaag gcacaccagt aaacacaagg gccgggcggt ggcggaccaa
      721 tgatcagggt caacggcagg gagatggagt ggcgccgggg catgactata caggatgtcc
      781 ttgatgagtg cggctatacc ttcagaatga tcgcagtgtg gatcgacggt actccctacc
      841 gcagagaaca tttcgccacc gccgtcgttc ctgacagagc ggaagtacag gctcttcata
      901 tgatcagcgg aggttagaca ctgtaatgct cttcggaaaa gaaccgacag gatttatctt
      961 cgatgtgaaa cggtactcta tccatgatgg accggggatc cgcacgacat ttttctttaa
     1021 aggatgccct ctttcctgtt ggtggtgcca taatccggaa ggcatttcac ctcttccggc
     1081 gcccacgtat catggaaaca ggtgtatagc ctgcggacga tgcgttcgtg aatgtcccaa
     1141 tggagcccga agcttcggcc ccgaaggaat tgcgctggac tcttcgaagt gtgtcgagtg
     1201 caggatatgc gagcaagttt gtcctacgcg tgctctcgag ttcgtcggca aagaaatgac
     1261 tgtggaagaa attgttcgcc tcgcgaaaaa ggatcttgct ttttatgacg aatcaggcgg
     1321 aggtgtcact ttctccggag gagagccttt gatgcaggcc gagtttctca tcgcgtgtct
     1381 ggaagcgctc cgctctctgg aaatttctac ggcagtggat acttccggct tctgtcgcga
     1441 ggaggatctt cttctcgccg cggaagaggc ggatattttt cttttcgaca tcaagctgat
     1501 tgacccgaag ctgcatgaat actatactgg tgtaggtaat gaaagaattt tagccaatct
     1561 gaggagcctc gacaaaaagc tcgtggaaca gggaagagga agcatcaata tcaggattcc
     1621 cctcattccc ggtgtgaaca attctccgga aaatctggag gccgtgattg catgcgttct
     1681 ttctctacga agcgtttcgg gcgtgaatat tttgccatat cactctatcg gatccggcaa
     1741 gtacaaa
//
LOCUS       DAOTIL010000650         1746 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminobacteriaceae bacterium isolate AMR_MDS_3026
            NODE_80941_length_1746_cov_3.079243, whole genome shotgun sequence.
ACCESSION   DAOTIL010000650 DAOTIL010000000
VERSION     DAOTIL010000650.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868050
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminobacteriaceae bacterium (wastewater metagenome)
  ORGANISM  Aminobacteriaceae bacterium
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae.
REFERENCE   1  (bases 1 to 1746)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1746)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:19:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,224
            CDSs (total)                      :: 3,198
            Genes (coding)                    :: 3,184
            CDSs (with protein)               :: 3,184
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1746
                     /organism="Aminobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_80941_length_1746_cov_3.079243"
                     /isolate="AMR_MDS_3026"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:3034609"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..781)
                     /locus_tag="P5201_11805"
     CDS             complement(<1..781)
                     /locus_tag="P5201_11805"
                     /inference="COORDINATES: protein motif:HMM:NF025694.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4392 domain-containing protein"
                     /protein_id="HRV99273.1"
                     /translation="MESFGRICSVIGSDRPGRKVGGLLRPEHLSRALELLIKASSVAV
                     VTGFFVPACGAPETDGPGGAVMLGRALERCGRSATLFTDARCFSVLESCSLAVEGPAV
                     RSVETGEEILESSPSLLVFIERLGRAADGRYYNMRAEDISSVTAPLDDAAPLALIAGI
                     PVLAVGDGGNEAGMGNFRGDLSSILPEYAHCLSVTQASVTLPADVSDWGGYALAAMLS
                     LAAGEWVGPEEEEIDRMLASLVGAGAVDGVTRRGEPTVDGFD"
     gene            complement(783..1742)
                     /gene="pfkA"
                     /locus_tag="P5201_11810"
     CDS             complement(783..1742)
                     /gene="pfkA"
                     /locus_tag="P5201_11810"
                     /EC_number="2.7.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006583161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphofructokinase"
                     /protein_id="HRV99274.1"
                     /translation="MKRIAVLTSGGDAPGMNAAVRAVVRTALYHGMETIGVMKGYEGL
                     MDGECITLDRAAVGGIIHRGGTILRTARSERFKTEAGLRAALVQMENRKVDALVVIGG
                     DGSFRGAEKLCAMGLPVIGIPGTIDNDIAGTDVTIGYDTALNTALSAVQKLRDTASSH
                     DRLFIVEVMGRDAGFLALDVAVSTGAEYVVVPEIPLDIKKMCDHLHYSRKRGKTHSLI
                     ILAEGVMSALDLRNQLQDTGGYDARVTVLGYIQRGGSPSSLDTILASRMGSFAVESLL
                     EGKTGVMVGSVCGAMSLCPLEDAWKAAKELNPELLALVDKLSV"
BASE COUNT          351 a          624 c          469 g          302 t
ORIGIN      
        1 cgtcgaagcc gtccaccgtc ggttcgcccc tgcgggtcac accgtccacg gcgcccgccc
       61 cgacaaggga agcgagcatc cggtcaatct cctcttcttc cggtccaacc cactcgccgg
      121 cggcaagtga aagcatggcg gcaagcgcat acccgcccca atccgacacg tcggccggaa
      181 gagtaaccga agcctgcgtc accgagaggc aatgcgcgta ttccggaaga atcgacgaaa
      241 gatccccgcg aaagttcccc atgcctgctt cattcccccc gtcgcctaca gcaagcacgg
      301 ggatccccgc tatgagcgca agaggcgcgg catcgtccaa cggcgcagtc acagaggaga
      361 tatcctcggc gcgcatgttg tagtaccgcc cgtcggcggc ccgcccaagc cgttcaatga
      421 aaacaagaag cgagggcgaa gattcaagaa tctcttcccc cgtttcgacg gaacggacag
      481 caggcccctc cacagccagg gagcaactct ccagaactga aaaacaccgc gcatccgtaa
      541 agagcgtcgc gcttcgtccg catcgttcca acgccctgcc gagcatcacc gctccgcccg
      601 gaccgtccgt ttccggagct ccgcacgcgg ggacaaaaaa cccagtcacc acagccacgg
      661 aggacgcttt tatcaacagc tccaaagccc gactgagatg ctccggacga agaagccctc
      721 cgacctttcg tccgggccgg tcggaaccta ttacggaaca aatgcggccg aaactctcca
      781 tatcacacgc tcagcttgtc gacgagcgcc agcagctcgg gattcagctc tttagcggcc
      841 ttccaggcgt cctccagagg gcagagcgac atggcgccgc acaccgagcc gaccatgact
      901 ccggtctttc cctcgagcag cgactccacc gcgaaggaac ccattctgga ggccagaatc
      961 gtgtccaacg acgacgggct tcctcctcgc tgaatatacc ccagaactgt cacgcgggcg
     1021 tcgtatcctc cggtgtcctg aagctgattg cgcagatcca gcgccgacat cactccctct
     1081 gcaaggatga tcagagagtg cgtcttcccg cgcttcctcg agtagtgcag atgatcgcac
     1141 atttttttga tgtccagagg gatctcgggg acgacgacgt actccgcccc ggtggaaacg
     1201 gcgacgtcga gcgcgaggaa ccccgcgtcg cggcccatga cctcgacgat gaagagcctg
     1261 tcgtggctgc tcgccgtatc cctgagcttc tggactgcgg agagcgccgt gttcagcgcg
     1321 gtgtcgtatc cgatggtcac atcggtgccg gcgatatcgt tatctattgt cccgggaatg
     1381 ccgatcaccg gcagccccat cgcgcacagc ttttcggctc ccctgaagga accgtcgccg
     1441 ccgatcacca ccagcgcatc caccttgcgg ttctccatct gcacaagcgc agcgcgaagc
     1501 ccggcttccg tcttgaaccg ctcgctccgc gccgtgcgca ggatcgtccc gcccctgtgg
     1561 ataatcccgc cgaccgcggc ccggtcgagc gtgatgcact cgccgtccat cagtccctcg
     1621 taccccttca tcacgcctat ggtctccatg ccgtgataga gcgccgtacg cacaacggca
     1681 cggactgccg cgttcatccc cggcgcgtct cctccgctcg tcaacaccgc gattcgtttc
     1741 atcata
//
LOCUS       DAOTIL010000651         1738 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminobacteriaceae bacterium isolate AMR_MDS_3026
            NODE_81418_length_1738_cov_4.212121, whole genome shotgun sequence.
ACCESSION   DAOTIL010000651 DAOTIL010000000
VERSION     DAOTIL010000651.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868050
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminobacteriaceae bacterium (wastewater metagenome)
  ORGANISM  Aminobacteriaceae bacterium
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae.
REFERENCE   1  (bases 1 to 1738)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1738)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:19:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,224
            CDSs (total)                      :: 3,198
            Genes (coding)                    :: 3,184
            CDSs (with protein)               :: 3,184
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1738
                     /organism="Aminobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_81418_length_1738_cov_4.212121"
                     /isolate="AMR_MDS_3026"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:3034609"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(145..1242)
                     /locus_tag="P5201_11815"
     CDS             complement(145..1242)
                     /locus_tag="P5201_11815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015557194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HRV99275.1"
                     /translation="MDIQSKSVTLEQVSKIFKDPQSGKDVHAVDGADFVIAPGELVTL
                     LGPSGCGKTTTLRMIAGFELPTKGKIRIGAEDITFLPPNKRDTAMVFQSYALFPHMNV
                     FDNVAYGLRLRKIPENEIQEKVMRFLGMVGLDTLAKRPPSRLSGGQQQRVALARSLIV
                     EPAVLLLDEPLSNLDALLREQMRVEIRRIQKSLGITAAYVTHDRVEAMSLSDRIIVMN
                     KGKIVQIGTPHQVYRDPVDAFVAGFIGKVAFFPAEVRGIDEVCTVDVRGKTFRLPRFS
                     EELKGAQRALVMCRPESLTLLPPGEGILDGRVTTNVYLGHSIESFVATEMGEILVQVD
                     NPDARRVFGEGEAVSVSFIPELTKALPAGEQ"
     gene            complement(1255..>1738)
                     /locus_tag="P5201_11820"
     CDS             complement(1255..>1738)
                     /locus_tag="P5201_11820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015557193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HRV99276.1"
                     /translation="PGTLVGISYILAFNKAPLILVGTGAIIVISYVIRELPVGVEAGI
                     ASLKQIDPAIEEAAADLGADTPTVFRTIVLPLIRPAFISSLSYTFVRSMTAVSAVIFL
                     ISARWYHLTVLIYNFSENLRFGLASVLASTLIVIVLLTFGLLRLLVRKNENLAKTMSL
                     "
BASE COUNT          323 a          504 c          579 g          332 t
ORIGIN      
        1 cccatgagca cgctctcgac ccgctgtatg ccccgccggt gatccgaaaa gcggagtgac
       61 gaaagcccgg tttttgacga tgtgcacaca aaaagcggag tcccggggtt tttccggggc
      121 tccgctttgt atatctttga acgattactg ctccccggca ggcagcgctt ttgtcagttc
      181 cgggatgaaa gagacggaga cggcttctcc ctctccgaag acgcgacgtg catcgggatt
      241 gtccacctgt acgaggatct ctcccatctc cgtggcgacg aacgactcga tgctgtgccc
      301 gaggtagacg ttggtggtga cgcggccgtc gaggatgccc tctcccggag gaagcagagt
      361 gagcgactcg gggcggcaca tgacaagggc gcgctgcgcg cctttcagct cttccgagaa
      421 gcgcggaaga cggaaggtct ttcccctgac gtcgaccgtg cagacttcgt cgatgccccg
      481 tacctccgcg gggaagaagg ccaccttgcc gatgaaaccc gcgacgaagg cgtccaccgg
      541 gtcgcggtag acctggtgcg gcgtgcctat ctgcacgatc ttccccttgt tcatgacgat
      601 gatcctgtcg gagaggctca tcgcctccac ccggtcgtgg gtgacgtatg cggcggtgat
      661 cccgagcgac ttctggatac ggcggatctc cacgcgcatc tgctcgcgga gcagcgcgtc
      721 gagattggag agcggttcgt cgagcagcag gacggcgggc tccacgatga gggagcgcgc
      781 aagggccacg cgctgctgct ggcctccgga gagacgggac ggggggcgct tggcgagcgt
      841 gtcgagcccc accatgccga ggaagcgcat gaccttctcc tggatttcgt tctccgggat
      901 tttccgaagg cgcagcccgt aggccacgtt gtcgaagacg ttcatatgcg ggaagagcgc
      961 gtagctctgg aagaccatcg ccgtatcccg tttgttggga gggaggaagg tgatgtcttc
     1021 ggcgccgatg cggatcttcc ccttcgtcgg cagttcgaac cccgcgatca tgcggagcgt
     1081 cgtcgtcttg ccgcacccgg acggaccgag cagcgtgacc agctctcccg gtgcgatgac
     1141 gaagtcggcg ccgtcgacgg catgcacgtc ctttcccgac tgcgggtcct tgaatatctt
     1201 gcttacctgt tcgagtgtga ctgatttcga ttggatgtcc ataactatcc caccttagag
     1261 actcatggtc ttcgcgaggt tttcgttctt ccgaaccaga agacggagga ggccgaacgt
     1321 caggagcacg atgacgatga gcgtgctcgc caaaacgctg gcaaggccga agcgcaggtt
     1381 ctcggagaag ttgtagatca gcacggtgag gtggtaccac ctggcggaga taaggaagat
     1441 gaccgcgctg accgccgtca tggagcggac gaaggtgtac gacagactcg agatgaaggc
     1501 gggcctgatg agcgggagga cgatggtgcg gaagacagtg ggcgtgtccg ccccgaggtc
     1561 cgcagcggcc tcctcgatcg ccgggtcgat ctgtttcagc gaggcgatgc ccgcttcgac
     1621 ccccaccggc agctctcgga tgacgtagct gatgacgatg atggcgcccg tcccgacgag
     1681 gatgagcggg gccttgttga aggcgaggat gtagctgata ccgaccagcg ttccggga
//
LOCUS       DAOTIL010000652         1738 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminobacteriaceae bacterium isolate AMR_MDS_3026
            NODE_81424_length_1738_cov_3.808675, whole genome shotgun sequence.
ACCESSION   DAOTIL010000652 DAOTIL010000000
VERSION     DAOTIL010000652.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868050
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminobacteriaceae bacterium (wastewater metagenome)
  ORGANISM  Aminobacteriaceae bacterium
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae.
REFERENCE   1  (bases 1 to 1738)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1738)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:19:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,224
            CDSs (total)                      :: 3,198
            Genes (coding)                    :: 3,184
            CDSs (with protein)               :: 3,184
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1738
                     /organism="Aminobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_81424_length_1738_cov_3.808675"
                     /isolate="AMR_MDS_3026"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:3034609"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..239)
                     /locus_tag="P5201_11825"
     CDS             complement(<1..239)
                     /locus_tag="P5201_11825"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRV99277.1"
                     /translation="MNAVERVRSFLQELWSLCPKLLLLWTLLLAGFSSAWMVTIADKE
                     MRGALLHEARLLAAAINPERVRLLAGDDSDLERES"
     gene            complement(305..1366)
                     /gene="murB"
                     /locus_tag="P5201_11830"
     CDS             complement(305..1366)
                     /gene="murB"
                     /locus_tag="P5201_11830"
                     /EC_number="1.3.1.98"
                     /inference="COORDINATES: protein motif:HMM:NF010480.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008762 - UDP-N-acetylmuramate
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate dehydrogenase"
                     /protein_id="HRV99278.1"
                     /translation="MASLFPLRNASEAPMDAASFPAVATAAAERIRALDVGTVSFREP
                     LKEHCSWEIGGPADIFVQPSEDAQVLRLLRFVREEGLPLVVIGRGTNLLFAAEGFRGV
                     VMKFGRRFSDFSVDGASIRARGGLWVPRLVKNLVEAGLSGLEHAAGIPGSFGGLVTMN
                     GGSLRHGVGERVVEVRAVSFAGETRAFSREECGFSYRNSFFQRASDSPESRWIVLEAR
                     LELVPAERNDIRREVLGILEERRGKFPLDKPNCGSVFTNDPSVYELAGPPGKIVEAAG
                     LKGLRVGGAEVSHRHANFIVNLGGATASDVLSLIAEIRRRVHDRIGVWLECEVRYVGA
                     DGTLVPASTASENLRPEGK"
     gene            complement(1341..>1738)
                     /locus_tag="P5201_11835"
     CDS             complement(1341..>1738)
                     /locus_tag="P5201_11835"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRV99279.1"
                     /translation="HLLSEGDEKGFSVVLEEKQQSRERKDELKWSSGLGDQVYLSMRL
                     ALASLWGKNSEPLPLILDDLLVRFDEGRQRGAAMAICEAARENQVMLFTCQKGTLDVF
                     RDVVAETGESGSDFLSFHRIENGEFVPVA"
BASE COUNT          327 a          600 c          536 g          275 t
ORIGIN      
        1 tacgactcgc gttccaggtc ggaatcgtct cccgcgagca accgcactcg ttcggggttg
       61 atcgccgcgg cgaggaggcg cgcttcatgc aggagagcgc ctcgcatctc cttgtcggcg
      121 atcgtcacca tccatgccga ggagaagccc gcgaggagga gcgtccacaa gagcagcagc
      181 ttcggacaga gcgaccaaag ctcctgcaag aagctgcgca cgcgttcaac agcgttcatt
      241 ggcttccccc ccgtttccgg aaatcggcgc cccggaagca cagtttcgct cgccgggggt
      301 cgtttcactt tccttcgggc cgcagatttt ccgaggctgt cgacgcagga accagagtcc
      361 cgtcggcgcc gacataacgg acttcgcact cgagccatac tccgatacgg tcgtgaacgc
      421 gcctgcgaat ctccgcgatg agggagagca cgtccgacgc ggtcgcgccg ccgaggttga
      481 cgatgaagtt ggcgtgccgg tgcgagacct ccgcgccgcc gacccgcagc cctttcaatc
      541 cggccgcttc gacgatcttc cccggagggc ccgcaagttc gtagacggac ggatcgttcg
      601 tgaagacgga gccgcagttc ggcttgtcga gcggaaactt cccgcgccgc tcctcgagga
      661 tgcccagcac ctccctgcgg atatcgttcc gctccgccgg gacgagttcg agacgggctt
      721 caaggacaat ccaccggctc tccggggagt ccgaggcgcg ctgaaaaaac gagtttctgt
      781 aggagaatcc gcactcctcg cgcgaaaagg cgcgcgtctc ccccgcgaag gagaccgccc
      841 gcacctcgac gacgcgttcg ccgaccccgt gccggaggct gccgccgttc atcgtgacga
      901 ggcccccgaa cgacccgggg atccccgccg cgtgctcaag gcccgagagt ccggcttcga
      961 caagattttt caccagacgc ggaacccaga ggcctccgcg ggcacggatc gaggcgccgt
     1021 cgacggagaa atcggagaaa cgccgtccga acttcatgac cacgccccgg aacccttcag
     1081 cggcgaagag aagattcgtt cccctgccga tcacgacgag ggggagcccc tcctcccgga
     1141 caaagcggag gaggcggagc acctgcgcgt cctccgaagg ctggacgaaa atatccgccg
     1201 ggccgccgat ttcccacgag cagtgctcct tcagcggctc ccggaaggag accgttccca
     1261 cgtcgagcgc gcggatcctc tcggccgccg ccgttgcgac ggccgggaac gaagccgcgt
     1321 ccattggcgc ttcggaggcg ttacgcaacg ggaacaaact cgccattctc gatgcggtgg
     1381 aaggagagga aatccgaccc gctctctccc gtctcggcga cgacgtcgcg gaagacgtcg
     1441 agcgtcccct tctgacaggt aaagagcatc acctggttct cgcgggcggc ctcgcagatc
     1501 gccatcgccg cgccgcgctg gcgcccctcg tcgaagcgca ccagaaggtc gtcgagaatc
     1561 agcggcaatg gctcggagtt cttcccccac agagaggcga gggcaagccg catcgaaagg
     1621 tagacctggt cgcccaggcc ggaactccac ttgagctcat ccttccgctc gcgggactgc
     1681 tgtttctcct ccagcaccac ggagaacccc ttctcatctc cttcggagag cagatggt
//
LOCUS       DAOTIL010000653         1730 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminobacteriaceae bacterium isolate AMR_MDS_3026
            NODE_82029_length_1730_cov_2.134925, whole genome shotgun sequence.
ACCESSION   DAOTIL010000653 DAOTIL010000000
VERSION     DAOTIL010000653.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868050
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminobacteriaceae bacterium (wastewater metagenome)
  ORGANISM  Aminobacteriaceae bacterium
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae.
REFERENCE   1  (bases 1 to 1730)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1730)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:19:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,224
            CDSs (total)                      :: 3,198
            Genes (coding)                    :: 3,184
            CDSs (with protein)               :: 3,184
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1730
                     /organism="Aminobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_82029_length_1730_cov_2.134925"
                     /isolate="AMR_MDS_3026"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:3034609"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            195..374
                     /locus_tag="P5201_11840"
     CDS             195..374
                     /locus_tag="P5201_11840"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRV99280.1"
                     /translation="MAISTAVQKGSTVYVYNEKNGQICAKSGTLMGFTSTTFSVKQGN
                     TVYTYNEKGGMISAH"
     gene            446..1537
                     /locus_tag="P5201_11845"
     CDS             446..1537
                     /locus_tag="P5201_11845"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012376.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair exonuclease"
                     /protein_id="HRV99281.1"
                     /translation="MVRFVHTADWQIGMKLAKIGDAGKRVREERFSAARRVIDAASAH
                     GAEFILVAGDLFEDNAVDRTQVRMVADILGKAPCPVFIIPGNHDPLVPGAVWEDRSWE
                     AARNVVVFSENAPYELPGATLYPCPLRGKQSDADPTSWIRAEGGDRIHIGLAHGSVEG
                     APIGEWDHPIPRDAASRSGLDYLALGHWHSFGLFGTKDAPRTAYSGTHEPTSFGERDS
                     GNALIVDIEAPGCAPVLTPVRTGGISWISIEEQIDSRDALESVRERIGTLQNPERTLL
                     RVRLSGLMRPDDRDALEEIALAAASFLWSEVDESGLLPSPEDDSWIGNLPAGLLREVG
                     KRLNQAQEAPEIRTKALLELYALLSEVKA"
     gene            1534..>1730
                     /locus_tag="P5201_11850"
     CDS             1534..>1730
                     /locus_tag="P5201_11850"
                     /inference="COORDINATES: protein motif:HMM:NF024867.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HRV99282.1"
                     /translation="MILHTLVVENWRCILGERSVGPFSEGVNILHAPNATGKSTLFEA
                     LRCALMDNHNTKGEEIQKLRP"
BASE COUNT          363 a          518 c          544 g          305 t
ORIGIN      
        1 tcggatcctg atgataccca tacttgccag cgcgttgtac tggttcgcgg aacacatctt
       61 tgcgatagcg gtcgtcatcg cgattctctt tttcttcttc aggatatgat cgcaaaccct
      121 cgtaagcggt cgcggtaatt gtgtagagtt aatggcggat gggacagtac ggaaaatcga
      181 caggaggaga tagcatggcg ataagcacag cggtacaaaa agggagcaca gtgtacgtgt
      241 ataacgaaaa aaacggccag atctgcgcca aatcgggcac gttgatgggc tttacctcga
      301 cgacgttcag cgtcaagcag gggaataccg tgtacacata caatgaaaaa gggggtatga
      361 tttccgcgca ttagtacgac gcgaagagct acggctcagg tgtattcgat gaagggcatc
      421 cggtgaatga aaaggagggg gttgcatggt tcgatttgtg cacacggccg actggcagat
      481 agggatgaag ctcgcgaaga tcggcgatgc ggggaagcgc gtccgggagg aacgcttctc
      541 cgccgcccgg agggtcatcg acgcggcgtc cgcacacgga gcggagttca tcctcgtcgc
      601 gggagatctt ttcgaggaca acgcagtcga ccgcacgcag gtgcggatgg tggccgacat
      661 cctcgggaag gctccatgcc cggtcttcat catccccggc aaccatgacc ccctcgtccc
      721 gggagccgtc tgggaggacc gttcctggga ggcggcccgc aatgtcgtcg tcttcagtga
      781 aaacgctccg tacgagcttc cgggcgcgac cctctacccc tgcccgctgc ggggaaagca
      841 gtccgacgcg gacccgacct cgtggatacg ggcggaaggg ggcgaccgga tccatatagg
      901 cctggcccac ggctccgtcg aaggagcgcc catcggcgag tgggaccacc cgatcccgag
      961 agatgcggcg tcgcgctccg gactcgacta ccttgcgctc ggccactggc actccttcgg
     1021 actgttcgga acaaaggacg ccccgaggac ggcctattcc ggaacgcacg aaccgacctc
     1081 gttcggcgag cgggacagcg gcaacgcgct catcgtcgac atcgaagcgc ccggatgcgc
     1141 ccctgtcctc acgcccgtcc gcaccggggg catctcctgg atatccatcg aggagcagat
     1201 cgactcccgg gatgccctcg aaagcgtccg cgaacgaatc ggcacgcttc aaaacccgga
     1261 gcgtaccctt ctccgcgtcc gcctttccgg actgatgcgg ccggacgacc gcgacgcgct
     1321 tgaggagatt gcgctggcgg cggcgtcttt tctctggagc gaagtggacg agagcggact
     1381 cctgccctcg cccgaagacg actcctggat cggcaacctg cccgcggggc tgcttcggga
     1441 ggtgggcaaa cgcctgaacc aggcgcagga agcgccggaa atcaggacga aggctcttct
     1501 cgaactctat gcccttctat cggaggtgaa ggcatgattc tgcacacact cgttgtcgag
     1561 aactggcgat gcattctggg agagcgctcc gtcgggccct tctccgaggg ggtgaacatc
     1621 ctccacgccc ccaacgcgac ggggaaatcg acgctcttcg aagcgctccg ctgcgccctg
     1681 atggataacc acaatacgaa gggggaagag attcagaaac tccgtccctg
//
LOCUS       DAOTIL010000654         1727 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminobacteriaceae bacterium isolate AMR_MDS_3026
            NODE_82189_length_1727_cov_4.041268, whole genome shotgun sequence.
ACCESSION   DAOTIL010000654 DAOTIL010000000
VERSION     DAOTIL010000654.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868050
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminobacteriaceae bacterium (wastewater metagenome)
  ORGANISM  Aminobacteriaceae bacterium
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae.
REFERENCE   1  (bases 1 to 1727)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1727)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:19:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,224
            CDSs (total)                      :: 3,198
            Genes (coding)                    :: 3,184
            CDSs (with protein)               :: 3,184
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1727
                     /organism="Aminobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_82189_length_1727_cov_4.041268"
                     /isolate="AMR_MDS_3026"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:3034609"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1727)
                     /locus_tag="P5201_11855"
     CDS             complement(<1..>1727)
                     /locus_tag="P5201_11855"
                     /inference="COORDINATES: protein motif:HMM:NF013631.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PLAT/LH2 domain-containing protein"
                     /protein_id="HRV99283.1"
                     /translation="PWNIPSPVARITLGPNKSPVADFRIRPQDPKWSPETILDASPSY
                     DPDGIITRYSWTLEAGSPPDARNDSVIAHRFPAGGVQTVTLSLTDNKGATSTVIKRVH
                     TSKPFTVRGDEFAAQSVFAVPPTASYEVTVRTGDRVKSGSDAGVFLALYGPADEQGTP
                     TGSGEIHLLPSSSLQCTSTDPFERGGTDVFTIPEIYGFGSLDEAERMTLRHDNGGDGA
                     GWFVDGVQVKNLKNGKSWTFPANRWLATDEGDGKTYAEFTPITGAYERGILFGGDARS
                     IGMIEASDRIFILPAGASSFYFTQLEGSRELRVYRNDVLVGTHSQAGRGLRDPLFLPE
                     SGWGVSYDASLITAPARFRVQTLDSGSVREDGSVWVLPQSWAGHEKTVRQAVMADALI
                     RELRSGLSGEDPYGVFACGKSAVKVFAAARPNLGVAGVKILRYGMSSLSIFGDFDDVQ
                     FDLFTELASNAYFSSKLPALVAQLLTGTFGSVTTEMFSLLKSIFNAAEWGAEFFSTSI
                     FDAPSDVYAVDLLNDIAVSDSFFLQSAELLQASGEKLRAAVSAFEQNDPEACATAFGS
                     MSTLVVGDAP"
BASE COUNT          344 a          572 c          517 g          294 t
ORIGIN      
        1 ggggcgtcgc cgaccacaag tgtggacatg ctgccgaagg ccgtcgcaca cgcctccggg
       61 tcgttctgct caaaagccga tacagccgcc cgcagcttct ctccggaagc ttgcagaagc
      121 tccgcggact gcaggaagaa actgtctgac acggcgatgt cgttcagcag gtcgaccgca
      181 tagacatccg aaggcgcgtc gaaaatcgac gtcgagaaga actccgcccc ccactccgct
      241 gcgttgaaaa tgctcttcag aagagaaaac atctccgtcg tcaccgagcc gaacgtacct
      301 gtcaaaagct gtgcaaccag cgcgggcaac ttgctcgaaa aataggcatt ggaagcgagt
      361 tcggtgaaca gatcgaactg cacatcgtca aaatcgccga agatcgaaag ggaggacatg
      421 ccgtaccgca ggattttcac tccggcaacc ccgaggttcg ggcgcgccgc ggcgaacacc
      481 ttcacagcgc tcttgccgca ggcaaagaca ccgtacggat cctctcccga caggccgctg
      541 cgaagctccc gaatcagagc gtccgccatc accgcctgcc ggaccgtctt ctcgtgtccg
      601 gcccagctct gcggcagcac ccagacagag ccatcctccc ggacggaacc tgaatcgagc
      661 gtctggacgc ggaagcgcgc cggagccgtg atgagcgacg cgtcgtacga aactccccag
      721 ccgctctccg ggaggaagag cgggtcgcga agcccccgcc ccgcctggga gtgcgtccct
      781 accagcacgt cgttccggta gacgcggagc tcccgcgatc cttcaagctg cgtgaagtag
      841 aaggacgaag cgcctgcggg caggatgaag atcctgtcgc tcgcctcgat catgccgatg
      901 gagcgggcgt ctcccccgaa gagaattccg cgctcgtagg cgcccgtaat cggggtgaac
      961 tctgcgtacg tcttgccgtc cccctcgtcc gtcgcaagcc agcggttcgc cgggaaggtc
     1021 cagctcttcc cgttcttcag attcttgacc tgaacgccgt ccacgaacca cccggctcca
     1081 tcaccgccgt tgtcatgacg aagcgtcatc cgctccgcct cgtcgagcga accgaatcca
     1141 tagatctccg gaatggtgaa gacatccgtc cctcctcgtt cgaacgggtc ggtggaggta
     1201 cactgaaggc tcgaactcgg gagcagatga atctcccccg atcccgtcgg cgtgccctgt
     1261 tcgtcggcag gaccgtagag ggcaaggaag accccggcgt cggagccgct cttcacacga
     1321 tcgccggtcc tcaccgtcac ctcgtacgac gctgtcgggg gcacggcaaa gacgctctga
     1381 gcggcgaact catccccccg caccgtaaag ggctttgagg tgtgtactct ttttatgaca
     1441 gtggatgtcg cccccttgtt gtcggtcaga ctcagcgtca ccgtctgtac gcctcccgcc
     1501 gggaagcggt gggcaatgac ggaatcgttc cgggcatcgg ggggactccc cgcctccagc
     1561 gtccaggagt accgggtgat gatcccgtcc ggatcgtacg acggcgaggc gtccagaatg
     1621 gtctcgggcg accacttcgg atcctgcggc cggatgcgga aatcggccac cggagacttg
     1681 ttgggtccga gcgtgatccg cgccacgggc gaagggatat tccacgg
//
LOCUS       DAOTIL010000655         1726 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminobacteriaceae bacterium isolate AMR_MDS_3026
            NODE_82281_length_1726_cov_2.682226, whole genome shotgun sequence.
ACCESSION   DAOTIL010000655 DAOTIL010000000
VERSION     DAOTIL010000655.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868050
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminobacteriaceae bacterium (wastewater metagenome)
  ORGANISM  Aminobacteriaceae bacterium
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae.
REFERENCE   1  (bases 1 to 1726)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1726)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:19:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,224
            CDSs (total)                      :: 3,198
            Genes (coding)                    :: 3,184
            CDSs (with protein)               :: 3,184
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1726
                     /organism="Aminobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_82281_length_1726_cov_2.682226"
                     /isolate="AMR_MDS_3026"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:3034609"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..722)
                     /locus_tag="P5201_11860"
     CDS             complement(<1..722)
                     /locus_tag="P5201_11860"
                     /inference="COORDINATES: protein motif:HMM:NF015909.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate--AMP phosphotransferase"
                     /protein_id="HRV99284.1"
                     /translation="MLEAVDMSVKMKKKEYRAAFDELAPKLGELQRIAKEKGVPVILL
                     VEGWEGSNKGSIVNQLLQTLDPRGYLFLAPPGRGEERYPFLIPYWEITPPAGKMTILS
                     GSWYREVLRRRLEKGEGAKDYSGVFRDICAFERHLADSGTVVLKFFCHLEKKEQKKRL
                     EARAADPDLSWSVGEADWLENREYDAFLFCAEEMLSFTGTEYAPWTVVAAGDFEFAAW
                     KILSTVERVLREVRGRVLVRLL"
     gene            complement(770..1189)
                     /locus_tag="P5201_11865"
     CDS             complement(770..1189)
                     /locus_tag="P5201_11865"
                     /inference="COORDINATES: protein motif:HMM:NF027567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase family
                     protein"
                     /protein_id="HRV99285.1"
                     /translation="MRDPEKTVGNLIDKSPVSLIGSVDEEGFPNTKAMLPPRKREGIK
                     RLYFTTNTSSMRVQQYGVNPKACVYFFDKRFFRGVMLKGTMEVLHDPETKETIWRDGD
                     TMYYPGGVTDPDYCVLRFTAESFRFYSDFKSESCAIE"
BASE COUNT          368 a          549 c          465 g          344 t
ORIGIN      
        1 tcgaggaggc gtaccagaac ccgccctcga acttcccgca gcacgcgctc gaccgtggag
       61 aggatcttcc acgcggcgaa ctcgaaatcc cccgccgcga ccaccgtcca cggcgcgtac
      121 tccgttcccg tgaaggagag catctcctcc gcgcagaaga ggaaggcgtc gtactccctg
      181 ttttccagcc agtcggcctc gcccaccgac caggagagat cggggtcggc ggcgcgggct
      241 tcaaggcgct tcttctgctc cttcttctcc aggtggcaga agaacttgag caccaccgtg
      301 ccgctgtcgg ccagatgacg ctcgaaagcg cagatgtcgc ggaagacgcc ggagtagtcc
      361 ttcgccccct cgcccttctc cagcctgcgg cggagcacct cgcggtacca gctccccgag
      421 aggatcgtca tctttcctgc gggcggcgtg atctcccagt acggaatcag gaagggataa
      481 cgctcctcgc cgcgccccgg aggcgcaaga aagaggtagc cgcgggggtc gagcgtctgg
      541 aggagctggt taacgatgct ccccttgttc gagccctccc acccttctac gagcaggatg
      601 acgggaaccc ccttctcctt ggcgatccgc tgcagctccc cgagcttcgg cgccagctcg
      661 tcgaaggccg ccctgtactc cttcttcttc atcttcaccg acatatccac cgcttcaagc
      721 attgttccgc ctcccttccg tagatttact caacgccgtc gtctgaaatt cactcgatcg
      781 cacaactctc ggacttgaaa tcgctgtaaa accggaaact ctcggcggtg aatcgcaaga
      841 cgcagtaatc ggggtccgtc acccctcccg gatagtacat cgtatcgccg tcccgccaga
      901 tagtctcttt cgtctccgga tcgtgcagca cctccatcgt gcccttcagc atgacgcctc
      961 tgaaaaagcg cttgtcgaag aagtagacgc aggccttcgg attcaccccg tactgttgca
     1021 cacgcatgga ggacgtattc gtcgtgaaat agagacgctt gatcccctct ctctttcggg
     1081 gagggagcat cgccttggta tttggaaacc cctcttcgtc gacagagccg atcagcgaca
     1141 ccggcgactt gtcgatcaag ttccccaccg tcttttcagg atctcgcact gcaaaggcct
     1201 ccttcgaaaa atcaggttct cttgcgaaca acgaataaaa gtccgccaga tcgcgcattc
     1261 gcggcgcgat ccgatccaac gatagaactg gagagttcct gccggcagct ctcgcagttt
     1321 tttaccccgt gaaagaaagt gccgtaaaag cattatccca cgacgcacgg tatacgcaag
     1381 ggaggaaaaa ccgccttttc cggaggaaga acgatgatgg cgcagagtgc ggtttcaaag
     1441 gagcgacgtc acaaggcggg cttatgctaa aatagtctgg aatatctctg gacaacgtcc
     1501 ctcgaaaacc gtttttcaac gtcgggcgaa caggcggctc tcacggacga ggaacaagga
     1561 ggaggatgcg tatgaaacag actgtggagg ccattgtcga tgagcgcggc aatgttcatt
     1621 tgacggaacc tctctccata acaggagttc gccgcgcact gctgacggtg cacgacgaac
     1681 cgccctccat cctcgacgag acgctgcttt cggcggagca aagctt
//
LOCUS       DAOTIL010000656         1725 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminobacteriaceae bacterium isolate AMR_MDS_3026
            NODE_82349_length_1725_cov_2.885629, whole genome shotgun sequence.
ACCESSION   DAOTIL010000656 DAOTIL010000000
VERSION     DAOTIL010000656.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868050
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminobacteriaceae bacterium (wastewater metagenome)
  ORGANISM  Aminobacteriaceae bacterium
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae.
REFERENCE   1  (bases 1 to 1725)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1725)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:19:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,224
            CDSs (total)                      :: 3,198
            Genes (coding)                    :: 3,184
            CDSs (with protein)               :: 3,184
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1725
                     /organism="Aminobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_82349_length_1725_cov_2.885629"
                     /isolate="AMR_MDS_3026"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:3034609"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>1725
                     /locus_tag="P5201_11870"
     CDS             <1..>1725
                     /locus_tag="P5201_11870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013047715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HRV99286.1"
                     /translation="PELSVKRKTTMLMVFLAAILLGGVVFIGLKLVLLPKIEPPVVNI
                     LTTWPGASAGDVEQSVSKRLEDSLSLIEGVDKIFSKSMDNISVVTVQFKWGEDLDVKM
                     GDIRDSVNFAKRDLPSDAEEPILLRITSGTIPFLTLSLTAKRSFEGLHHFAQSVVVEN
                     ISRIPGVGQVLVYGGDQREIQIRIDAEKLEAFSIPMTSIIAAVERENLDIPAGSLKEG
                     ATEYYIRVPGRFSSVDEIGKTVVGAAGGVLVHLNDVAEVVDTYKDPDIQGYHFDERAV
                     IMAVLKTSDANTVEVSRAVLARLEELKEKEFPADVDYHIGMNTSEFILNSINNLSQSL
                     IAGIVLVIAVTWIFLKRFSASLIVCAAIPFSLVITFIFMGEMDYTINIFTLSALAMAS
                     GMVVDNCIVATDQIVYHLEKGARKSVASIVGTGEVQSAMIASTLTTVVVLLPLAFIRG
                     LVGVFFSSLTVVMVVAVTASLFVSLSFIPMMASVFFRREEDRLFIHRFSDRIIGRLER
                     GYEDLLEWSLSNRKKVLFTALTLLALTATGFRFIGTELSPDPDTGEITITMTLPEGTR
                     IDETDALVL"
BASE COUNT          333 a          508 c          498 g          386 t
ORIGIN      
        1 cccgagcttt cagtcaaacg caagacgacc atgctgatgg tctttcttgc ggcgattctc
       61 ctgggaggcg tcgtcttcat cgggttgaag ctcgtcctcc tccccaaaat cgagcctccc
      121 gtagtgaaca tcctcaccac ctggccgggc gcctcggcgg gggacgtgga gcagagcgtc
      181 tcgaagaggc tggaggattc gctctcgctc atcgaaggcg tggataaaat attctccaag
      241 tcgatggaca acatctccgt ggtcaccgtc cagttcaagt ggggagagga tctcgacgtc
      301 aagatgggcg atatacggga ttccgtcaac ttcgcgaagc gcgacctccc ctccgatgcg
      361 gaagaaccga ttttgctgcg catcacctcg ggaacaatcc ccttcctcac gctctcgctc
      421 acagcgaagc gctcttttga ggggctgcat cattttgcgc agagtgtggt cgtcgagaat
      481 atctcccgca tccccggagt ggggcaggtg cttgtctacg gaggcgacca gagggagatt
      541 cagatacgta tcgacgcgga gaagctggag gcgttcagca ttcctatgac ctccatcatc
      601 gccgccgtcg agagggagaa cctcgatatt cccgccggct ctttgaaaga aggggcgacg
      661 gagtattaca tccgcgttcc cggacgcttt tcctccgtcg acgagatagg caaaacagtc
      721 gtcggagcgg cgggaggcgt gctcgttcat ttgaacgacg tggcggaggt tgtggatacc
      781 tacaaagatc ccgacattca agggtaccat ttcgacgagc gcgccgttat catggcggtt
      841 ctcaagacga gcgacgccaa caccgtcgag gtgagccgtg cggtgctcgc gcggctggaa
      901 gagctgaagg aaaaggagtt tcccgcggat gtcgactatc atatcggcat gaacacctcc
      961 gagttcatac tgaactccat aaacaacctg tcgcagtcgc ttattgcggg tattgtcctc
     1021 gttatcgccg tgacatggat ctttttgaag aggttttccg cctcgctcat cgtctgcgcc
     1081 gccatcccct tttctctcgt catcaccttc atttttatgg gggagatgga ctacacgatc
     1141 aacattttca cgctctccgc gctcgcgatg gcgagcggca tggtcgtgga caactgcatc
     1201 gtggccaccg accagatcgt ctaccacctt gaaaagggag cgcggaagag tgtcgcgagc
     1261 atcgtcggaa ccggcgaagt gcagtccgcg atgatcgcct ccaccctcac gactgtagtg
     1321 gtgctgcttc cgctcgcctt cattcgcggc cttgtcggcg tcttcttctc gtcgctgacg
     1381 gtcgttatgg tcgtcgcggt gactgcgtct ctcttcgtca gcctctcctt catccccatg
     1441 atggcgagcg tctttttccg gcgggaggag gaccgtctgt tcatccaccg tttctcggac
     1501 cgaatcatcg ggcgcctgga gagggggtac gaggaccttc tcgagtggag cctctcgaac
     1561 cggaaaaaag tgctcttcac cgcgctgacg cttcttgcgc tgacggcgac gggcttccgt
     1621 ttcatcggca cggagctctc ccccgatccg gatacgggcg agatcaccat tacgatgacg
     1681 cttcccgagg ggacgaggat cgacgaaacc gacgcgcttg tgctc
//
LOCUS       DAOTIL010000657         1725 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminobacteriaceae bacterium isolate AMR_MDS_3026
            NODE_82362_length_1725_cov_2.450299, whole genome shotgun sequence.
ACCESSION   DAOTIL010000657 DAOTIL010000000
VERSION     DAOTIL010000657.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868050
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminobacteriaceae bacterium (wastewater metagenome)
  ORGANISM  Aminobacteriaceae bacterium
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae.
REFERENCE   1  (bases 1 to 1725)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1725)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:19:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,224
            CDSs (total)                      :: 3,198
            Genes (coding)                    :: 3,184
            CDSs (with protein)               :: 3,184
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1725
                     /organism="Aminobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_82362_length_1725_cov_2.450299"
                     /isolate="AMR_MDS_3026"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:3034609"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(298..1605)
                     /locus_tag="P5201_11875"
     CDS             complement(298..1605)
                     /locus_tag="P5201_11875"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HRV99287.1"
                     /translation="MKQTERPSFMPWKTQYSRCATETPRPSPTQKNTAAEESVFSVAA
                     LCAETLKALHQRIRKRKPRIELDIDSSIPRLARGNGAQIENILHLLLSNVIALSLDAA
                     VKLSVVTRRFTAEGLEVLFSIEWAHSGVALSADPVIEGNAPPGLSYSICSSVIAAMGG
                     RLHVKKQEENMVISFFIPLSTAGISEDAENGGAPVLLVEDNAMNRKIIASSLEHGGYS
                     VFTASSGQEALNALFDKEWSIILMDIQMPGMDGIQTTHAIRAEEKKKGRRTPIVAVTA
                     YARPEDRERCLAAGMDDFLAKPVKLETLLSTVERVTGIPSARKSAVRGPDAASAILSF
                     TEGDAELARKIIGEFLALAPPLLLSMRDAFDRKDRETLRVLSHRLKGNLAYFGEGEAF
                     LLAEALHRELDNLSDEAVAATLLRLLSESEYLIEALENKEEAA"
BASE COUNT          348 a          465 c          518 g          394 t
ORIGIN      
        1 aagagttttc accttctctg gttgcggcgg gtcactgtac gggattccgt gcgcaagccg
       61 cgctgtatgc ggcattcgat gaaaagttta caactatggg agccggcgcc gtcttcgcct
      121 tcaaggcgta atggcccgtt ctattggaaa aactgtaccg cgctgcttcc gactgtcgcg
      181 ggactgatcg cagggaagct tgcgggtggt atgaggaagg gctagaccaa aaagccggtt
      241 gtagatgata ccagccggca ggtaataaag aaaagcaagg ggaagcgtcc gaagttatca
      301 ggctgcttcc tctttgtttt ccagtgcttc aataaggtat tcgctctcag aaagaaggcg
      361 gagcaaggtt gccgcgactg cttcgtcgga gaggttgtcg agctcccggt gcagcgcttc
      421 cgcaagaaga aaggcttctc cttcgccgaa ataggccaga ttccccttga gtctgtgaga
      481 gagcacacgg agcgtctccc tatccttccg gtcgaaggca tcgcgcatcg agagcagcag
      541 cggcggggcg agcgcgagga actcgccgat gattttccgc gccagctcgg catccccttc
      601 cgtaaaggag agaatggccg aggcggcgtc cggtcccctt accgcgctct ttctggcgga
      661 ggggatgccg gtgactctct cgacggtgga gagaagcgtc tccagtttga ccggttttgc
      721 gaggaagtcg tccatccccg cggcaaggca gcgctctcta tcctcgggtc tggcgtacgc
      781 ggtgacggcg actatcggag tgcgccgccc ctttttcttc tcctcggcgc ggatggcgtg
      841 agtcgtctgt attccgtcca ttcctggcat ctggatatcc atcagaatga tgctccactc
      901 tttgtcgaag agggcgttaa gcgcttcctg accggaagaa gcggtgaaaa ccgagtaccc
      961 tccgtgttcg aggctggacg cgatgatctt cctgttcatc gcgttgtcct ccacaagcag
     1021 aaccggcgcg ccgccgttct ccgcatcctc ggagataccg gcggttgata acggaataaa
     1081 aaacgagatg accatgttct cttcctgttt tttcacgtgc aaacggccgc ccattgcagc
     1141 gatgaccgac gagcaaattg aataactcag gccgggcggc gcgtttcctt caataacggg
     1201 atcggccgac aaagcaacgc cgctgtgtgc ccattcgatg gaaaaaagaa cttccagccc
     1261 ctcggctgta aaccttcgtg tgacaacgga gagttttacg gcggcgtcca aactcaacgc
     1321 gatgacgttc gagagaagaa ggtgaagtat gttctctatc tgcgctccat ttcccctcgc
     1381 caagcggggg atggaggagt cgatgtcgag ctcgatgcgc ggcttccttt ttcgaatacg
     1441 ttgatgaagc gctttcaggg tttcggcaca gagagcggcg acagaaaaaa cgctctcttc
     1501 cgccgcggta tttttttgtg tgggcgaggg gcgcggcgtc tcggttgcac accggctgta
     1561 ctgggtcttc cacggcataa aggatggacg ttcggtctgt ttcatacaag taactccgat
     1621 ctgatgctct cgcgcaggag atagcgtttc agcggccttc cggggccgtt gtattcgaac
     1681 tggattctga cggcgcctgt tttggagagg tggtcgaggt acctc
//
LOCUS       DAOTIL010000658         1722 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminobacteriaceae bacterium isolate AMR_MDS_3026
            NODE_82539_length_1722_cov_4.187762, whole genome shotgun sequence.
ACCESSION   DAOTIL010000658 DAOTIL010000000
VERSION     DAOTIL010000658.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868050
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminobacteriaceae bacterium (wastewater metagenome)
  ORGANISM  Aminobacteriaceae bacterium
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae.
REFERENCE   1  (bases 1 to 1722)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1722)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:19:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,224
            CDSs (total)                      :: 3,198
            Genes (coding)                    :: 3,184
            CDSs (with protein)               :: 3,184
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1722
                     /organism="Aminobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_82539_length_1722_cov_4.187762"
                     /isolate="AMR_MDS_3026"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:3034609"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            159..536
                     /locus_tag="P5201_11880"
     CDS             159..536
                     /locus_tag="P5201_11880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005661321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase family
                     protein"
                     /protein_id="HRV99288.1"
                     /translation="MALLPEDVGRAWEKRKGAVVLTTVDKDGVPNSIYATCVSKYDEE
                     TIVVADNYFDKTQKNILAGSKGSVLFITDEGASFQIKGRIEYLKEGPVFDDMKKWNPT
                     KHPGHAAAALKVEEVYSGAKKLL"
     gene            663..1649
                     /locus_tag="P5201_11885"
     CDS             663..1649
                     /locus_tag="P5201_11885"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0052621 - diguanylate cyclase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor domain-containing diguanylate cyclase"
                     /protein_id="HRV99289.1"
                     /translation="MAHKPTYEELEAQVLRLKDAERELREMEEMLQDKITLWQLLIEQ
                     SKDGIVVLDENGSVYAANKRFAEMLGYTEEELRRLKIWEWDGRLSKEQLLGNLRRANT
                     SGHSLETLHRCKNGAIIDVELSYNGALYNGRKLVFCVCRDISARKRMEQELRESEQKY
                     RELCIVDGLTNLYNARHFHSHLKMESNRADRYGQPLSLLFFDLDDFKKYNDVYGHVEG
                     DKVLSRIGEVVKRGLRQTDSAFRYGGEEFAVILPMTDLPSAITAAERLADELYREVFS
                     PASGEDVHITCSIGVALHRQNEDPVSFVQRADLLMYRAKSEGKNRVCAESVL"
BASE COUNT          414 a          496 c          520 g          292 t
ORIGIN      
        1 gcttcatgga cttcgtccac gcgggcgaga tgatcgagga gggacgccgt gcggcaagag
       61 aggcgctgaa ataacggctg ggcacggcgc ggtattcgtg gtattgttcc tactgagctg
      121 caccataccg ctactttact ttaatggagg aaaacaacat ggccttacta ccggaagacg
      181 tcggcagagc gtgggagaag agaaagggag cggtcgtcct gacgaccgtc gacaaggacg
      241 gcgtaccgaa ttcgatttac gctacatgcg tgagcaagta cgacgaggag acgatcgtcg
      301 tcgcggacaa ctacttcgac aagacgcaaa agaacattct cgcgggaagc aagggctccg
      361 tgctcttcat caccgatgag ggcgcgtcct ttcagatcaa ggggcgcata gagtatctca
      421 aagagggccc ggtcttcgac gacatgaaaa aatggaaccc gaccaagcat ccgggacatg
      481 ccgcagcggc gttgaaggtg gaagaggtct attcgggcgc aaagaagctg ctgtaatcaa
      541 ccggccgcgt ccgcgctgct ctgcgacggc ccggccggtt tcagtatttt ccccgtctgc
      601 gcgaacaagc gaacgcctgt ataatgctct tcctgaaccg acaaaaaagc gaggtgagcg
      661 tcatggcgca caaaccgacc tacgaggagc tggaagcgca ggtactgcgg ttgaaggacg
      721 cggaacgcga actccgtgaa atggaagaga tgctgcagga caagatcacg ttatggcagc
      781 ttttgatcga acagtcgaag gacggcatcg tcgtcctcga cgaaaacggg tcggtctacg
      841 cggccaacaa acgcttcgcc gaaatgctcg gctacacgga agaggagctg cgccggctca
      901 aaatctggga gtgggacggc cgcctctcga aggagcagct gctggggaac ctccggcgcg
      961 caaatacatc cggccattct ctggagacgc tccaccgctg caagaacggc gcgattatcg
     1021 acgtcgaact cagctacaac ggcgcgctct acaacgggcg aaagctggtg ttctgcgtgt
     1081 gcagggacat ctcggcacga aaacggatgg aacaggagct cagggaaagc gaacagaaat
     1141 acagggaact ctgcatcgtc gacggcctca cgaacctcta caacgcacgc cacttccaca
     1201 gtcatctgaa gatggagagc aaccgcgccg accgctacgg acagccgttg tcactcctct
     1261 tcttcgacct cgacgacttc aagaagtaca acgacgtcta cggtcatgtc gagggagaca
     1321 aggtgctctc gcggatcggg gaggtcgtca agcgcggtct ccgtcagacc gattccgcct
     1381 tccgctacgg gggcgaggag ttcgccgtca ttctgccgat gacggatctc ccgtcggcga
     1441 tcactgcggc ggaacgtctc gccgacgagc tgtaccgcga agtcttctcg cctgcatcgg
     1501 gagaggacgt ccatatcaca tgcagcatag gcgtggcgct gcatcgacag aacgaagacc
     1561 ccgtgagctt cgtccaacgc gccgacctct taatgtaccg ggcgaagagc gaggggaaaa
     1621 accgcgtctg cgccgaatcc gtcttgtaag aatgggagaa cgctcactgc ggtcttcacc
     1681 acccgccgcc accgccgccg cctcgcccgc ctccggaaaa gc
//
LOCUS       DAOTIL010000659         1722 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminobacteriaceae bacterium isolate AMR_MDS_3026
            NODE_82555_length_1722_cov_3.272346, whole genome shotgun sequence.
ACCESSION   DAOTIL010000659 DAOTIL010000000
VERSION     DAOTIL010000659.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868050
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminobacteriaceae bacterium (wastewater metagenome)
  ORGANISM  Aminobacteriaceae bacterium
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae.
REFERENCE   1  (bases 1 to 1722)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1722)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:19:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,224
            CDSs (total)                      :: 3,198
            Genes (coding)                    :: 3,184
            CDSs (with protein)               :: 3,184
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1722
                     /organism="Aminobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_82555_length_1722_cov_3.272346"
                     /isolate="AMR_MDS_3026"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:3034609"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(169..978)
                     /locus_tag="P5201_11890"
     CDS             complement(169..978)
                     /locus_tag="P5201_11890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012868838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cobalamin adenosyltransferase"
                     /protein_id="HRV99290.1"
                     /translation="MKLITEQDLRAEIGGKDLRSYRVDRGTIVTPSARQYLSDRKIEL
                     LVGDEAPAQPKETIPPATLPPQGAEAAEPKRARFIGPDGGTFTEKPEHLTHLSGNRLV
                     TKGHPRIAFRGRMDSLQSKIMELQVRAVELGNGVLAEELDELLDFARSLLAAEVTGRP
                     VDDVPLLGLELDEIRAMSHNPKKTFGLGHIRPHYSMGALCVGLNALRSEVREVELAAF
                     RAFSGSDGALDSGQDMPDRRLDVLRALNRLSSAFYILMYRHLPSGYNREFK"
     gene            complement(1009..>1722)
                     /locus_tag="P5201_11895"
     CDS             complement(1009..>1722)
                     /locus_tag="P5201_11895"
                     /inference="COORDINATES: protein motif:HMM:NF024122.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="haloacid dehalogenase-like hydrolase"
                     /protein_id="HRV99291.1"
                     /translation="WVLYLFSGMTADEVKALAEKSNDAALADKLETYTLSSSKTLQSK
                     AGYISMGGYKRGLRTVPEMSNLMNVLRSHGIHVYVCTASLDNVVRVFSNLPKYGYNVP
                     EENVLGMRVAMEDGKYVPRYDDTWVQTQQAGKTEAIKRELVSKYGYGPIAVFGDSQGD
                     YKMCVDFEETQVVLVVNRLRKDDFGKLGLQAMETHGKPDAKFILQGRDENIGAFRPDE
                     KTIKYGKTEPELFHASLVK"
BASE COUNT          287 a          570 c          511 g          354 t
ORIGIN      
        1 ccgtcacgaa aacaacaccc caaatctttt acaacaagtg atcaccaatg tttttctcaa
       61 tcccggaaag tgcgcccagg ggcacggcgc actaagcgat cgacgcaact cgcaaaatca
      121 gctcgttggt cgcctgctta tcgttggtcg cctgcttacg ttggtcgcct acttaaattc
      181 tctgttatac ccagatggca gatgcctgta catcaggatg tagaaggcgc tgctgaggcg
      241 gttgagggcg cgcaggacgt ccagacgcct gtccggcatg tcttggccgg agtcgagcgc
      301 gccgtcgctt ccggagaagg cgcggaatgc cgccagctcc acctcgcgga cctccgagcg
      361 cagggcgttc agcccgacgc agagcgcgcc catcgagtag tgcggacgga tgtgccccag
      421 tccgaaggtc tttttcggat tgtgggacat cgccctgatt tcgtcaagct ccagcccgag
      481 aagcggcaca tcgtccaccg gacgccccgt cacctcggcg gcaaggagcg agcgcgcaaa
      541 gtcgagcagt tcgtccagtt cctcggcgag cacgccgttg cccagctcga cggcgcgcac
      601 ctgcaattcc atgatcttcg actggaggct gtccatcctc cccctgaacg cgatgcgggg
      661 gtgccccttg gtcaccagcc ggttgcccga gaggtgcgtc aggtgctccg gcttctcggt
      721 aaaggttcct ccgtccgggc cgatgaaacg ggccctcttc ggctccgcgg cctccgcccc
      781 ctgagggggg agggtcgccg gaggtatcgt ctctttcggc tgcgccggcg cctcgtcccc
      841 taccaggagc tctatcttcc ggtcgctgag gtactggcga gccgaagggg tgacgatcgt
      901 cccccggtca acccggtagg agcgaagatc cttgccgcct atctcggccc gcagatcctg
      961 ttctgtgatc agcttcattc cagtcacccc ccggcatccg gccgcgcgct acttcaccag
     1021 gcttgcgtga aagagctccg gctccgtctt gccgtatttt atcgtctttt cgtcggggcg
     1081 gaaagcgccg atgttctcgt cgcgcccctg aagaatgaac ttcgcgtcgg gctttccgtg
     1141 cgtttccatc gcctgcaggc caagcttgcc gaagtcgtcc ttgcggaggc ggttcaccac
     1201 gaggacgacc tgcgtctcct cgaagtccac gcacatcttg tagtctccct ggctgtcgcc
     1261 gaagacggcg atgggaccgt agccgtactt gctcacaagt tcgcgcttga tggcctcggt
     1321 cttccccgcc tgctgcgtct gcacccacgt atcgtcgtag cgcgggacgt acttcccgtc
     1381 ctccatggcc acgcgcatgc cgagcacgtt ctcctcggga acgttgtagc cgtacttcgg
     1441 caggttggaa aagacgcgga ccacattgtc gagcgacgcg gtgcagacgt acacgtgaat
     1501 gccgtgcgaa cggagcacgt tcatcaggtt ggacatctcc ggaaccgtcc gcaggccgcg
     1561 cttgtagccg cccatgctga tataccccgc cttgctctgc agcgtcttgg agctggagag
     1621 cgtgtaggtc tccagcttgt cggcgagcgc cgcgtcgttg gacttctcgg cgagcgcctt
     1681 cacctcgtcc gcagtcatcc cgctgaagag gtagagcacc ca
//
LOCUS       DAOTIL010000660         1721 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminobacteriaceae bacterium isolate AMR_MDS_3026
            NODE_82612_length_1721_cov_3.972389, whole genome shotgun sequence.
ACCESSION   DAOTIL010000660 DAOTIL010000000
VERSION     DAOTIL010000660.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868050
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminobacteriaceae bacterium (wastewater metagenome)
  ORGANISM  Aminobacteriaceae bacterium
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae.
REFERENCE   1  (bases 1 to 1721)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1721)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:19:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,224
            CDSs (total)                      :: 3,198
            Genes (coding)                    :: 3,184
            CDSs (with protein)               :: 3,184
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1721
                     /organism="Aminobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_82612_length_1721_cov_3.972389"
                     /isolate="AMR_MDS_3026"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:3034609"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1363
                     /locus_tag="P5201_11900"
     CDS             <1..1363
                     /locus_tag="P5201_11900"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRV99292.1"
                     /translation="VGSSPAGDGRVFLRGKQMEVAPVDVARSKGWISSKGSKGVEEDE
                     LIGKLSDVSLTTCNERSPHYRLVSKSVVFVPGKRIIAKNPKVYIGEHLLFSYPFDYVV
                     PLDPKERRALLSSFFPLILSDSDKGQGVGIGGPYVWDTGKALLNVAWWSDTGWEGKAT
                     VEQRLNDYWTVWATVEHSYEKESAEKLYRPSWGILHAANGWNASLRWTQREAISIQKK
                     VGETYRGVLWREPEFSLLGPWGRAPLLNAYGRIGVSWGEYEDVQPTVSMNVRRLGAEV
                     QLYSEEQLESYKTFVKSEYKRFWYDDPDSLEQEIFDSVFGIRYAWGTVDLATAYVRRW
                     VSGETPMLWDYYRDREDLYQQVVFPAGKNLSFRIRGGYDLIDSKLAEMVYSVTLKGGE
                     CSRWELTYRNDRAEGDDWVGLSFFITAFPDTPVSFGRRELYDPFALPDGLAEVLRRDE
                     EEQ"
     gene            complement(1411..>1721)
                     /locus_tag="P5201_11905"
     CDS             complement(1411..>1721)
                     /locus_tag="P5201_11905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013048848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0032784 - regulation of DNA-templated
                     transcription elongation [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="GreA/GreB family elongation factor"
                     /protein_id="HRV99293.1"
                     /translation="KLETRISRLEFQLSNAKILDSSSIDASRVTLGTTVTIQDIINSQ
                     KFAYTIVGSEESDPKASKISSSSPVGQALLNKTVGDEVHVKVPRGVRHLRVMDISIIS
                     "
BASE COUNT          353 a          433 c          557 g          378 t
ORIGIN      
        1 tgtcggatcg tctcctgcag gcgacggaag ggtgtttttg cgcggaaagc agatggaggt
       61 tgcgcccgta gacgttgccc gcagtaaagg gtggatttcc tccaagggaa gcaagggagt
      121 tgaagaggac gaactgatcg gcaagctgag cgacgtgtcg ctgacgacgt gcaacgaacg
      181 ctcgcctcac tatcgcctgg tctcgaaaag cgttgtcttc gtccctggga aacggatcat
      241 cgcgaagaat ccgaaggtct atatcgggga gcaccttctc ttttcctacc ccttcgacta
      301 cgtcgtgccc cttgacccca aggagcggcg ggctttgctc agctcttttt tcccgcttat
      361 cctgagcgat tcggataaag gacagggagt gggcatcggc ggcccttatg tctgggatac
      421 tggcaaagcg ctgctgaacg tcgcctggtg gtccgatacc ggatgggagg ggaaggcgac
      481 tgtggagcag cggctgaacg actactggac cgtatgggcg acggtggagc actcctatga
      541 aaaagagtct gcggagaagc tctacaggcc gtcatggggc atcctgcacg cagcgaacgg
      601 atggaatgcc tctctccgtt ggacgcaacg cgaagcgatc tccatccaga aaaaggtcgg
      661 agagacctac aggggcgttc tgtggagaga acccgagttc tctctgctgg gaccgtgggg
      721 cagggcgcct ctgctcaacg cgtacgggag aataggggtt tcgtggggag agtacgagga
      781 cgttcagccg actgtcagca tgaatgtccg ccgtctcggg gcggaggtcc agctctactc
      841 ggaggagcag ctcgaatcct acaagacgtt cgttaagagc gagtacaaac ggttctggta
      901 cgacgacccg gattctcttg aacaggagat attcgactcc gtcttcggaa tccggtatgc
      961 ctggggaacc gttgatcttg caacggcgta tgttcgaaga tgggttagcg gcgagacgcc
     1021 gatgctgtgg gattattacc gcgaccgtga ggacctgtac cagcaggttg ttttcccggc
     1081 tggaaagaac ctctcgttca ggattcgcgg cggatacgac cttattgatt ccaaattggc
     1141 ggagatggtg tattcggtaa ccttgaaggg cggggagtgt tcccgctggg agcttaccta
     1201 cagaaacgac agggccgaag gagacgactg ggtcggcctc tcttttttca tcacggcgtt
     1261 cccggatact ccggtttcgt tcggacggcg cgaactgtat gatccgttcg ctctgccgga
     1321 tgggctggcg gaggttctcc gcagggacga agaggagcag taaacgaatg ctcctcctcg
     1381 tccctgtgaa gtgtgaaagg gctgaacccg tcaggatatg atggaaatgt ccatcaccct
     1441 gagatgccgc accccgcggg gaaccttcac gtggacttca tctccgacgg tcttgttgag
     1501 aagcgcctgt cccaccgggc ttgaagagga tatcttgctt gccttggggt cggactcttc
     1561 cgaaccgacg atcgtatagg cgaacttctg cgaattgatg atgtcctgga tggtgacggt
     1621 ggtcccgagg gtaaccctgc tggcgtcgat gctggaggag tcgaggattt ttgcgttgct
     1681 gagctggaat tcgagtcgag agatgcgcgt ttccagcttg g
//
LOCUS       DAOTIL010000661         1719 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminobacteriaceae bacterium isolate AMR_MDS_3026
            NODE_82760_length_1719_cov_3.026442, whole genome shotgun sequence.
ACCESSION   DAOTIL010000661 DAOTIL010000000
VERSION     DAOTIL010000661.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868050
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminobacteriaceae bacterium (wastewater metagenome)
  ORGANISM  Aminobacteriaceae bacterium
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae.
REFERENCE   1  (bases 1 to 1719)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1719)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:19:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,224
            CDSs (total)                      :: 3,198
            Genes (coding)                    :: 3,184
            CDSs (with protein)               :: 3,184
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1719
                     /organism="Aminobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_82760_length_1719_cov_3.026442"
                     /isolate="AMR_MDS_3026"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:3034609"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..474
                     /locus_tag="P5201_11910"
     CDS             <1..474
                     /locus_tag="P5201_11910"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRV99294.1"
                     /translation="PENISFLVPCAPYIDPVKGTPVASLQKGDFVLVRLPEDEAAPIR
                     EKLQRADPDFDGTVRGEVISVHKDRHGNFTLLVRLSDEFNGVTTLEGNSRIRTAPLAQ
                     PATVLPGTAAGSKEKEETPDLPQPSPGDTPGWVYFFVFGAMATLVVILFFIRNFL"
     gene            complement(455..1105)
                     /gene="thiE"
                     /locus_tag="P5201_11915"
     CDS             complement(455..1105)
                     /gene="thiE"
                     /locus_tag="P5201_11915"
                     /EC_number="2.5.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014807462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004789 - thiamine-phosphate
                     diphosphorylase activity [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine phosphate synthase"
                     /protein_id="HRV99295.1"
                     /translation="MNTTERIQRALKVYVIPDSTAGAPRSLEEQARLAILGGATMIQL
                     RDKKMTGRELYETAKRLLSLCRPRDIPLIVNDRLDVALASGADGVHLGAEDLPVHAAK
                     RFVPEGFIVGATAHSVEEGIRAEAEGADYVGVGAAFPSGTKKGAAVLGVAGIREVCSA
                     ISLPTVAIGGITAQNAREVLDAGVDGVSVVASVVGQEDIQAAASLLAKAVIRGSYE"
     gene            complement(1102..>1719)
                     /locus_tag="P5201_11920"
     CDS             complement(1102..>1719)
                     /locus_tag="P5201_11920"
                     /EC_number="2.7.1.50"
                     /inference="COORDINATES: protein motif:HMM:NF014198.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004417 - hydroxyethylthiazole kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyethylthiazole kinase"
                     /protein_id="HRV99296.1"
                     /translation="NTGAPSEDRELLIRNALTAAEVKRSATLLDPVGYGAASFRRQLV
                     DALLRDFRFAVVKGNRGEISLLAGEKGDVKGVDSEGSYASIVRSVRTVAVKTRSLVCA
                     TGETDVLSDGDAVLSVRGGSALLGRITGGGCVLGALMLTLSTAASSISAGVVCASVLL
                     RLAAERAEKISNGPGTFQAHLLDALHKTHPEELLNEAWRVEEVTE"
BASE COUNT          366 a          564 c          428 g          361 t
ORIGIN      
        1 cccgagaata tctcatttct cgttccttgc gctccctata ttgatccggt aaaggggact
       61 ccggtagcct cgttacaaaa aggggatttc gtgctggtac gtctccccga agatgaagcg
      121 gctcccatcc gggagaagtt acagcgtgcc gaccccgatt tcgacggtac agtacgcgga
      181 gaagtcatat cggtccacaa agaccggcac ggcaacttca ctctgctggt ccgactctcg
      241 gacgaattca acggggtaac aaccttggaa ggcaacagcc gcatacgcac cgctccgctc
      301 gcgcagcccg caaccgttct tccgggaacg gccgcaggct cgaaagaaaa agaggaaacg
      361 ccagatcttc ctcagccttc tcccggcgat actccgggtt gggtgtattt tttcgtgttc
      421 ggcgctatgg ctacccttgt cgttatcctc ttttttattc gtaacttcct ctgataacag
      481 ccttcgcgag gagagacgcg gctgcttgta tatcttcctg tccgactacc gaagcgacga
      541 ccgaaacccc atccacacct gcatctaaaa cttcacgggc attctgggct gtgataccgc
      601 ctatggcaac cgtcggaagg gaaatcgcgg aacaaacctc acgtatcccg gcaacgccga
      661 gaacagcggc tccttttttc gttcctgaag gaaatgccgc tcctacgccg acataatcgg
      721 cgccttcggc ttccgcccgt atcccttcct ccacagagtg cgccgtcgcc cctacaatga
      781 atccttcggg aacaaagcgt ttcgccgcat gcaccggaag atcctcggct cccagatgca
      841 ccccgtccgc tcctgaggcg agggcaacat ccaaacggtc gttgacgatt aacggaatat
      901 cgcgcggacg gcacaatgaa agaagacgct tcgccgtttc atagagttcg cgtcccgtca
      961 tcttcttgtc ccgcagctgt atcatcgtcg cgccgcccag aatcgccagc cgggcctgct
     1021 cttcgagaga gcgaggcgct cccgcagtgc tgtcgggaat aacgtacacc ttcaacgctc
     1081 tttgtattcg ttccgttgtg ttcattccgt tacctcctcc actctccacg cttcgttgag
     1141 caactcctcc ggatgcgtct tgtgcaaggc atccaacaag tgcgcctgaa aggttccggg
     1201 accgttcgag atcttttccg cgcgttccgc cgccagccga aggagaacgg aagcacagac
     1261 aaccccggcg gagatactgg aagccgccgt actcagggta agcatcaacg cgcccaacac
     1321 gcaccctccg cctgtgatcc gtccgagcaa cgcgctgcct ccccgcaccg acaacacggc
     1381 atcgccgtcg ctcagcacat ccgtctcgcc ggtagcgcag acgagcgaac gcgtctttac
     1441 tgcaactgtt ctgacggaac ggacaatgga cgcgtagctc ccctctgaat ccaccccctt
     1501 cacgtcaccc ttctctcccg ccaaaagaga gatctcgccc cggttcccct tgacgactgc
     1561 aaaacgaaaa tctcggagaa gcgcgtcgac aagctgtctg cgaaatgaag cagcgccgta
     1621 tcctacggga tcaagcagcg tagcgcttct ttttacctct gcggcggtaa gcgcgttccg
     1681 gataagcagt tctctatcct ccgagggagc gcctgtatt
//
LOCUS       DAOTIL010000662         1717 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminobacteriaceae bacterium isolate AMR_MDS_3026
            NODE_82880_length_1717_cov_3.517449, whole genome shotgun sequence.
ACCESSION   DAOTIL010000662 DAOTIL010000000
VERSION     DAOTIL010000662.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868050
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminobacteriaceae bacterium (wastewater metagenome)
  ORGANISM  Aminobacteriaceae bacterium
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae.
REFERENCE   1  (bases 1 to 1717)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1717)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:19:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,224
            CDSs (total)                      :: 3,198
            Genes (coding)                    :: 3,184
            CDSs (with protein)               :: 3,184
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1717
                     /organism="Aminobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_82880_length_1717_cov_3.517449"
                     /isolate="AMR_MDS_3026"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:3034609"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            236..562
                     /locus_tag="P5201_11925"
     CDS             236..562
                     /locus_tag="P5201_11925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013048200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell envelope biogenesis protein TolA"
                     /protein_id="HRV99297.1"
                     /translation="MAVNLAEEIKVVESRSRETVKDAKNEAARMVNEAKSETERRVKE
                     AKQKAFRQYKEKIASVEKEAEEKAQKTVEAGRKNAENLAQQFGKEVPSTAKWIAEEVI
                     SRYGRG"
     gene            549..>1717
                     /locus_tag="P5201_11930"
     CDS             549..>1717
                     /locus_tag="P5201_11930"
                     /inference="COORDINATES: protein motif:HMM:NF013647.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0033179 - proton-transporting V-type
                     ATPase, V0 domain [Evidence IEA];
                     GO_function: GO:0015078 - proton transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATPase 116kDa subunit family protein"
                     /protein_id="HRV99298.1"
                     /translation="MAVAKIAKAEFYIHKSDVDGVLSALQNTGSYEVVPFSSDDESEG
                     PVITHPDFSRVDFLLGETRFLLRFLEPHFVDPISGMARSMGEKEVFSLHQTRDLDSRT
                     DIPALAEDIRKLERRLMEIRSESSQIDSTEILLLSLKNFPYPLELLSTGTEHVRGILG
                     TIPAEQAEPWRRGAESLLGTMGEVYVAPGAEKEKERTVALFFDEAVSQKASELNARHS
                     FNRIEIPPGLQSGVSDELALLGKRRIALAAEEKEILGKSAEAAALWVPTVRALSDYYS
                     VLRDRYSALGSGNATDQVVMLRGWIPESALPEVQKALSAHDSSIELVLSEPGPDDNVP
                     SFIVNPPWCLPFENLTRLYGAPKYGEIDPTPLLAPFFFIFFGMCLGDAGYGLIMIG"
BASE COUNT          432 a          444 c          432 g          409 t
ORIGIN      
        1 cattagcttc ccaagctaag ggtcgcgggt tcgaatcccg ttgtccgctc caatttcttt
       61 cctttttccc caaactttgt ttcgctaatc agaatgttta ttttcgataa ttcctctatg
      121 ctctggacat attgttaagt ttgacacgaa ttttatgtag taataaaata ctctatgttt
      181 ttatgttgag actttggaca tcgaacaaac tttttttcga aagggggtgg agtgtatggc
      241 agtaaatctg gctgaggaga tcaaggtagt cgaatctcgt tccagggaga cggtcaagga
      301 tgcgaagaac gaagctgccc gaatggtcaa cgaagcgaaa agcgaaaccg aacgcagggt
      361 gaaggaagcc aagcagaaag ccttccgcca gtacaaggaa aaaatagcct ccgttgaaaa
      421 ggaagccgaa gagaaggcgc aaaaaaccgt tgaagccgga agaaaaaacg cggaaaacct
      481 cgctcagcag ttcggaaaag aagtcccgag cacggcgaaa tggattgcgg aagaggtgat
      541 ttctcgatat ggccgtggct aaaatagcaa aagccgagtt ttacatccac aagtccgacg
      601 tggatggggt tttatctgcc cttcaaaata ccggatcgta cgaggtagtc cccttcagtt
      661 cggatgatga atcggaagga ccggtcatta cccatccaga tttttccagg gtggattttc
      721 ttcttggaga aacacggttt ctcctccgtt tcctggaacc gcattttgtc gatcccattt
      781 ccggaatggc ccgctccatg ggagagaaag aagttttttc actccaccaa accagggatc
      841 ttgattcccg gacagacatc cctgcacttg cagaggatat ccgcaaactc gagcgcaggc
      901 tcatggaaat ccggtctgag tcttcgcaga ttgattccac ggaaattctt cttctttcac
      961 tgaagaattt tccatacccc ctcgaactgc tctccacggg aacagaacac gtcaggggca
     1021 ttctgggcac catacccgca gaacaggcgg aaccctggag acggggggcg gagtctcttc
     1081 tcggcactat gggtgaagtc tatgtcgctc cgggagcgga gaaagaaaaa gagcgcactg
     1141 ttgccctctt tttcgatgaa gcagtctccc agaaagcatc ggagctcaac gcccggcact
     1201 catttaaccg tattgaaatt ccgcccgggc ttcagtccgg ggtttctgac gaacttgccc
     1261 tcttaggcaa aagaagaatc gctctcgcgg cagaggaaaa agaaatcctg ggcaagtccg
     1321 ccgaggctgc cgccctatgg gttccaaccg tgcgtgccct gagcgactat tattccgttc
     1381 ttcgcgacag gtacagcgca ctgggctctg gaaacgccac cgatcaggtt gtcatgctcc
     1441 ggggatggat acccgaatcg gctcttccag aagtgcaaaa agccctttcc gcccatgact
     1501 cctcgatcga actcgttctt tccgaaccgg ggcccgatga caacgttccg tccttcattg
     1561 tcaacccgcc atggtgcctc cccttcgaaa acctcacccg gctgtacgga gctccaaaat
     1621 acggagaaat tgatccgacg ccgctccttg caccgttttt ctttattttc ttcgggatgt
     1681 gtctcggaga tgccgggtat ggcctgatca tgatcgg
//
LOCUS       DAOTIL010000663         1717 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminobacteriaceae bacterium isolate AMR_MDS_3026
            NODE_82894_length_1717_cov_2.903730, whole genome shotgun sequence.
ACCESSION   DAOTIL010000663 DAOTIL010000000
VERSION     DAOTIL010000663.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868050
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminobacteriaceae bacterium (wastewater metagenome)
  ORGANISM  Aminobacteriaceae bacterium
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae.
REFERENCE   1  (bases 1 to 1717)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1717)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:19:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,224
            CDSs (total)                      :: 3,198
            Genes (coding)                    :: 3,184
            CDSs (with protein)               :: 3,184
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1717
                     /organism="Aminobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_82894_length_1717_cov_2.903730"
                     /isolate="AMR_MDS_3026"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:3034609"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..909)
                     /locus_tag="P5201_11935"
     CDS             complement(<1..909)
                     /locus_tag="P5201_11935"
                     /inference="COORDINATES: protein motif:HMM:NF012589.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FGGY family carbohydrate kinase"
                     /protein_id="HRV99299.1"
                     /translation="MNVYLGLDIGTTNIKMLLVDERGSVVKSMSHPLELSAPRPAWAE
                     QDPEDWWNGVWTLLSSVPSDMNVLRIGLSGQMHTLVPVDSEGNVLRKAILWCDQRTSA
                     ECDEATAAVGGEEKIIEMTGNLIYPGFTLPKILWIRKNEPEVYSRISTCFIAKDYIGW
                     RLTGERAAEWSDASGSSCYDVSARRWRSDLLRTLGIDEAWLPSVKGSSDIRGKLRPEL
                     ASKLGWKQAEVVCGGADNAVAALGLGVCSPGDCMISIGTSGTVLAATESTSPDPTGRL
                     HFFNTVLPGQSYYMGCMLAAAASLNWF"
     gene            complement(929..>1717)
                     /locus_tag="P5201_11940"
     CDS             complement(929..>1717)
                     /locus_tag="P5201_11940"
                     /inference="COORDINATES: protein motif:HMM:NF014653.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ldh family oxidoreductase"
                     /protein_id="HRV99300.1"
                     /translation="RTIAKAKEHGTCMTVVQDSCHFGMAGFWAEKAADAGMMGVALTN
                     TLRAGIPLFGKERLLGTNPICVAIPTGKKPHFMLDMATTTVALGKIEVAARRGGTMPV
                     GWAVDENGADTTDAISVAEINRFDKSPFGGQLYLGGAGETLGGHKGYGLGLLVELLTA
                     GLSLGTPTYDTYINSCGICHYFQATRLDLFGNEEKIKAHVASILEKLRESPKAAEKER
                     IYIHGEKEFERRKKSMEEGVYLDPATWERLDEFADKFGMRRLEP"
BASE COUNT          277 a          539 c          551 g          350 t
ORIGIN      
        1 gaaccagttc agtgaggcgg ccgcggcgag catgcacccc atgtagtagg actgcccggg
       61 aaggacggtg ttgaagaagt gcagtcttcc cgtaggatcc ggcgatgtcg actctgtggc
      121 ggcgagcacc gttcccgaag tgccgatgga gatcatgcag tcgccgggcg agcagacgcc
      181 gagcccgagc gcagcgaccg cgttgtccgc gccgccgcac acaacctcgg cctgtttcca
      241 gcccagtttc gaggcaagtt ccgggcggag tttcccgcgt atgtccgagg agcccttcac
      301 cgagggcagc cacgcctcgt cgatgccgag cgtgcgcagc aggtcgctcc gccagcgccg
      361 ggcggagaca tcgtagcacg aacttccgga ggcgtccgac cactccgccg cccgctctcc
      421 ggtgagccgc cagccgatat agtccttggc gataaagcat gtactgatgc ggctgtagac
      481 ttcgggttcg ttcttgcgga tccagaggat cttcgggaga gtgaatccgg ggtagatcag
      541 gttcccggtc atctcaatga tcttttcctc tcctccgacg gcggcggtcg cctcgtcgca
      601 ttcggcggac gttctctggt cgcaccagag gatcgccttg cgaagaacgt ttccctcgct
      661 atcgacgggg acgagcgtat gcatctgccc cgagaggccg atgcggagga cgttcatgtc
      721 ggaggggacg gaagagagga gcgtccatac gccgttccac cagtcttccg ggtcctgctc
      781 ggcccaggcc gggcggggcg cggagagttc gagtgggtgc gacatgctct tgacgacgct
      841 cccccgttcg tccacgagga gcatcttgat gttcgtggtg ccgatgtcca ggccaagata
      901 gacgttcata cggtcccctc cctttggttt acggttcgag acggcgcata ccgaacttgt
      961 cggcgaattc gtcgagacgc tcccatgttg ccgggtcgag gtagacgccc tcttccatgg
     1021 atttcttccg gcgctcgaac tctttctcgc cgtggatgta gatacgctcc ttttccgctg
     1081 ccttggggct ttcgcgcagc ttttccagga tggaggcgac gtgcgccttg atcttctcct
     1141 cgttgccgaa gaggtcgagt cgtgtggcct ggaagtagtg gcagattccg cagctgttga
     1201 tgtacgtgtc gtacgtcgga gttccgaggg agagccctgc ggtgagcagc tcgacgagca
     1261 ggccgagacc gtaccccttg tgtcctccga gggtttctcc cgcgccgccg agatagagct
     1321 ggcctccgaa gggactcttg tcgaaacggt tgatctcggc cacgctgatc gcgtccgtgg
     1381 tatcggcgcc gttttcatcg acggcccagc cgacaggcat cgtgccgccc ctccgcgccg
     1441 ccacctcgat cttgccgagc gccacggtcg tcgtcgccat gtcgagcatg aagtgcggct
     1501 tcttccccgt ggggatggct acgcagatcg ggttcgtccc caaaaggcgc tccttaccga
     1561 agagggggat gcccgcccgg agggtgtttg taagcgcgac gcccatcatg ccagcgtcgg
     1621 cggctttctc agcccagaag cccgccatgc cgaaatggca cgaatcctgc acgacggtca
     1681 tgcaggtgcc gtgctccttc gccttcgcga tcgtgcg
//
LOCUS       DAOTIL010000664         1714 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminobacteriaceae bacterium isolate AMR_MDS_3026
            NODE_83102_length_1714_cov_3.555154, whole genome shotgun sequence.
ACCESSION   DAOTIL010000664 DAOTIL010000000
VERSION     DAOTIL010000664.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868050
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminobacteriaceae bacterium (wastewater metagenome)
  ORGANISM  Aminobacteriaceae bacterium
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae.
REFERENCE   1  (bases 1 to 1714)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1714)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:19:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,224
            CDSs (total)                      :: 3,198
            Genes (coding)                    :: 3,184
            CDSs (with protein)               :: 3,184
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1714
                     /organism="Aminobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_83102_length_1714_cov_3.555154"
                     /isolate="AMR_MDS_3026"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:3034609"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..407
                     /locus_tag="P5201_11945"
     CDS             <1..407
                     /locus_tag="P5201_11945"
                     /EC_number="4.2.1.126"
                     /inference="COORDINATES: protein motif:HMM:NF003915.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="N-acetylmuramic acid 6-phosphate etherase"
                     /protein_id="HRV99301.1"
                     /translation="GVTCNRNSAVGAAADIAIEIDVGPEVLTGSTRLKAGTAQKLALN
                     MISTMAMVGIGKVYKNLMVDVQRTNEKLRTRAVNIVADAAGVDKETAREFLEKAEGSV
                     KHAIVMLLLGCPLDEARAKLEAAKGRVRLALQ"
     gene            514..>1714
                     /locus_tag="P5201_11950"
     CDS             514..>1714
                     /locus_tag="P5201_11950"
                     /inference="COORDINATES: protein motif:HMM:NF014437.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HRV99302.1"
                     /translation="MEANTVLAGRILELVGGKGNVSSAANCMTRIRMRLKDASKADVA
                     GLKATDGVLGVVEGATLHVIVGPGKAKKVADILVEEFGVARDSAVTEDWEENKAAVKQ
                     GQKKGPLKTALETIAGIFIPLIPAIIAAGIFNGVGSLLATMLKQQAIAGPGWEFARLI
                     CQLGGSAFLGYFAIFTGVNAAKRFGATEALGGMVGAMTVGAQINEISQLLGLFDKDVP
                     LNSILITGKGGIIGVILGVYILAKVEKRVRKLVPDVLDLVFTSLITLLIVALGMVFVI
                     MPVSGYFSDWLVEALGLIINSDNTVVSIISGYVLAALFLPMVLLGLHHGLIPIYAIQL
                     QNFGGVSLFPVLAMAGAGQVGAAVAIWLKARKVKNTRIQSVIAGALPAGVLGIGEPLI
                     YGVTLPLG"
BASE COUNT          300 a          543 c          572 g          299 t
ORIGIN      
        1 tgggcgtcac gtgcaataga aactctgcgg tgggcgctgc ggcggacatc gccatcgaga
       61 tcgacgtcgg cccggaagtg ctcaccggct ccacgcggtt gaaggcgggg acggcgcaga
      121 agctggcgct caacatgatc tccacgatgg cgatggtggg cataggcaag gtgtacaaga
      181 acctgatggt cgacgtgcag cggacgaatg aaaagctccg gacgcgggcc gtcaacatcg
      241 tcgccgacgc tgccggggtc gataaggaaa ccgcccggga gttcctcgaa aaggcggagg
      301 ggagcgtgaa gcacgctatc gtcatgctgc tgctcggctg cccgctcgac gaggctcggg
      361 cgaagctgga ggcggcgaag gggcgcgtgc ggctcgccct gcagtgagtt tttcccgcag
      421 ctccgcgtcg ttgcgcaaag agccgcttca ccaggacggc cttcgcgcaa cgacgcggag
      481 cgcggtattt caacaggaga ggtgtgtgac ttaatggaag cgaacacggt attggccggc
      541 aggattctgg agcttgtcgg cggcaagggg aacgtctcct cggcggcgaa ctgcatgacg
      601 cggattcgca tgcgcctgaa ggacgcctcg aaggcggacg tcgccggact gaaggcaacc
      661 gacggggtgc tcggcgtggt ggagggggcg acgcttcatg tcatcgtcgg ccccggcaag
      721 gcgaagaagg tcgcggacat tcttgtcgag gagttcggcg tcgcgcgcga cagcgccgtg
      781 accgaggact gggaggagaa caaggccgcg gtcaagcagg ggcagaagaa gggaccgctc
      841 aagacggccc ttgaaaccat cgcgggcatc ttcatccccc tcattccggc gatcatcgcg
      901 gcgggcatct tcaacggcgt gggcagcctc ctcgccacca tgctgaagca gcaggcgatc
      961 gcggggcccg ggtgggagtt cgcccgcctg atctgtcagc ttggaggctc ggccttcctc
     1021 ggctacttcg ccatcttcac cggcgtcaac gcggcgaagc gcttcggcgc gaccgaggcg
     1081 ctcggcggca tggtcggcgc catgacggtc ggggcgcaga tcaacgagat ctcgcagctc
     1141 ctcggactct tcgacaagga cgtcccgctc aactccatcc tcatcaccgg caagggcggc
     1201 atcatcggcg tcatcctcgg cgtgtacatt ctcgccaagg tggaaaagcg cgtgcgcaag
     1261 cttgttcccg acgtactcga cctcgtcttc acctccctga tcacgctcct gatcgtcgcg
     1321 ctggggatgg tcttcgtcat catgcccgtc tccggctact tctccgactg gctggtcgag
     1381 gcgctcggcc tgatcatcaa ctccgacaac accgtcgtga gcattatctc cggctacgtc
     1441 ctcgccgcgc tcttccttcc catggtcctg ctggggctgc accacgggct catccccatc
     1501 tacgccatcc agctccagaa cttcggcggt gtctccctct tccctgtgct cgcgatggcc
     1561 ggggcggggc aggtcggcgc ggcggtcgcc atctggctga aagcacggaa agtgaagaac
     1621 acgcgtatcc agagcgttat cgcgggcgcg ctccccgcgg gcgtgctcgg catcggcgag
     1681 cccttgatct acggcgtcac actccctctg ggca
//
LOCUS       DAOTIL010000665         1714 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminobacteriaceae bacterium isolate AMR_MDS_3026
            NODE_83111_length_1714_cov_3.112116, whole genome shotgun sequence.
ACCESSION   DAOTIL010000665 DAOTIL010000000
VERSION     DAOTIL010000665.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868050
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminobacteriaceae bacterium (wastewater metagenome)
  ORGANISM  Aminobacteriaceae bacterium
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae.
REFERENCE   1  (bases 1 to 1714)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1714)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:19:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,224
            CDSs (total)                      :: 3,198
            Genes (coding)                    :: 3,184
            CDSs (with protein)               :: 3,184
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1714
                     /organism="Aminobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_83111_length_1714_cov_3.112116"
                     /isolate="AMR_MDS_3026"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:3034609"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            450..>1714
                     /locus_tag="P5201_11955"
     CDS             450..>1714
                     /locus_tag="P5201_11955"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRV99303.1"
                     /translation="MKIIARRKRERTTESAEARNEEMVLSSEAEHRAEGGLSPEESSS
                     ETSSDATGCSVPSTPSEEKETIDRDSMWKTIMVGNFDHMLDSILPELAAKVDRSRRPE
                     FLDKEMKDLKALMGGTWQEPDLLAKVPVKEGRDIWLALHVEVQDKGGEDFPERMFYYH
                     SMIRFKYLKRKRYEEEQAAKASVQSDGKRKMAKERRGVVDVVSMAILTARRPKGEAEF
                     YEHASYGNEMRYIYPAVRLWELDSEQLVASFNPFDLALLAAKRMIESGRSDNKRIIFL
                     KHLGSLLDERGWSRERSVALYRFIEWILRPKSEKKYEEYKAWVRKEERKVYITAAEEI
                     GMEKGLEKGVEIGKEIGKEIGKEIGKEIGKEIGKEIGKGEGILIGKEDTKREAALRML
                     RKGMEVSLVAECVDLPEDDPRRGFAHHGR"
BASE COUNT          425 a          409 c          536 g          344 t
ORIGIN      
        1 gattttgctc atttggagaa tcagcgcaga gaagttatca tgatttcttg tttgtaccaa
       61 aagctttttt cttgcctatt gggatcgtcg tccaattgcc caatacgaca ttgggcaatt
      121 catgggaaac acactctctc cggtttatta gctcgacagc cgcagattct ccaggggtct
      181 tttcttcttc gcgtccattc cgcctgagtt tgtaatggtc tgttcctcgt gcacgtcgtt
      241 gctcactgcg tcgcctcctt tgcaggtata atgatagtac atcatgatgc gtgcatactg
      301 ggtggtttat ggaaagagtg aaggtaacta gccaggcgct gtgattgcag aagtccgcct
      361 cttttcgtat aatattcgtg aaacacaaaa cgcacgctat cgagaagtag cggaagaccc
      421 cctgcgaggg gcatcattcg cgaggaggct tgaagattat cgcacggcgg aagagagaac
      481 gaacgaccga gagcgccgaa gctcgcaatg aagagatggt tctctcttcg gaggccgagc
      541 accgggcgga gggtggcttg agccctgaag aatcctcttc ggaaacatct tcggacgcga
      601 cgggctgtag cgtaccctcg acgccctcgg aggagaaaga gacgatcgac cgtgattcca
      661 tgtggaagac gattatggtg gggaacttcg accacatgct cgactccatt ctccccgaac
      721 tggccgcgaa ggtcgaccgc tcgcgccggc cggagttcct cgacaaagag atgaaagatc
      781 ttaaggcgct catgggcggc acgtggcagg agcccgacct tctcgccaag gtcccggtga
      841 aggaagggcg cgacatctgg ctcgctctcc acgtcgaagt gcaggacaag ggcggggagg
      901 acttccccga acgaatgttt tactatcatt ccatgatccg cttcaagtat ctgaagcgga
      961 agagatacga ggaggagcag gcggccaagg cctccgtgca gagcgacggg aagcggaaga
     1021 tggcgaagga gcgccgtgga gtcgtggacg tggtctcgat ggctatcctc acggcgcgca
     1081 ggccgaaggg agaggccgag ttctacgagc acgcctccta cggcaacgag atgcgttaca
     1141 tctatccggc ggtcaggctc tgggaacttg actccgaaca gctcgtagca agtttcaatc
     1201 ctttcgatct cgcgcttctt gcggcgaaga ggatgatcga aagcggccgc tcggacaaca
     1261 aacggatcat ttttttgaag catctcggaa gtcttctgga cgagcgcggg tggtctcgcg
     1321 aacgcagcgt cgcgctctac cgcttcatag aatggatact tcggccgaag agtgagaaga
     1381 agtacgaaga gtacaaggca tgggtaagga aggaggagag gaaagtgtac atcactgcag
     1441 ccgaggagat aggcatggag aagggattgg aaaagggcgt ggagatcgga aaggagatag
     1501 ggaaagagat aggaaaagag ataggaaagg agataggaaa ggagatagga aaagagatag
     1561 gaaagggcga aggcatcctc atcgggaagg aggataccaa gcgtgaagcc gctctcagga
     1621 tgcttcgcaa agggatggag gtttccctcg ttgcggagtg cgtcgacctc ccggaagacg
     1681 atccgcgccg cgggttcgcg caccacggcc gtga
//
LOCUS       DAOTIL010000666         1714 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminobacteriaceae bacterium isolate AMR_MDS_3026
            NODE_83131_length_1714_cov_2.569017, whole genome shotgun sequence.
ACCESSION   DAOTIL010000666 DAOTIL010000000
VERSION     DAOTIL010000666.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868050
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminobacteriaceae bacterium (wastewater metagenome)
  ORGANISM  Aminobacteriaceae bacterium
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae.
REFERENCE   1  (bases 1 to 1714)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1714)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:19:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,224
            CDSs (total)                      :: 3,198
            Genes (coding)                    :: 3,184
            CDSs (with protein)               :: 3,184
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1714
                     /organism="Aminobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_83131_length_1714_cov_2.569017"
                     /isolate="AMR_MDS_3026"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:3034609"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..801
                     /locus_tag="P5201_11960"
     CDS             <1..801
                     /locus_tag="P5201_11960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009164555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lyase family protein"
                     /protein_id="HRV99304.1"
                     /translation="GQEISGWARMLERDEEMIRSGLEFLRELALGGTAVGTGLNAHPD
                     FAASVAEELSMLSGKEFRTAPNKFHSLTSKDELVFVHGALKALAGDLMKIANDIRWLA
                     SGPRCGLGELVIPENEPGSSIMPGKVNPTQCEAVTMVAVQVMGNDAAVGFAASQGNFE
                     LNVFMPVLIYNFLQSCRLLGDVMDSFERNAVRGMEADTKRIRTYVNESLMLVTALTPR
                     IGYDKAAEIAKKAHKEGSTLKEAAVALHYLTEDEFDSAVVPEKMVGPR"
     gene            complement(1016..>1714)
                     /locus_tag="P5201_11965"
     CDS             complement(1016..>1714)
                     /locus_tag="P5201_11965"
                     /inference="COORDINATES: protein motif:HMM:NF012382.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HRV99305.1"
                     /translation="VKLVFLCSPHNPVGRVWSREELEKFGAICAAKGVVVVSDEIHCD
                     ILYKGAKHTPLASVSDAMRESTITTVAPSKTFNIAGLATAAVVIPSKRLRDNYQSVID
                     FVNIDGGNVFGATALEAAYTKGDAWLDELLRYLEGNLDFIDTFLAEHLPEVSLLRPEG
                     TYVPLLDCRSLNLSGPELERLLVEKGKVALDGGHWFGTGGDGFARINIATPRALLKEG
                     MEGIEKALKGYRNR"
BASE COUNT          381 a          451 c          513 g          369 t
ORIGIN      
        1 gggcaggaga taagcggatg ggctcgtatg ctcgaaagag acgaagagat gattcgctcc
       61 ggactcgaat tcttgagaga gttggcgctc ggaggaacgg ctgtcggaac cggactgaac
      121 gctcaccctg attttgccgc ttctgttgcg gaggagcttt ccatgctttc gggaaaagag
      181 ttccgaacgg cgccgaacaa gttccattcg ctgacaagta aagatgaact ggtgtttgtc
      241 cacggcgcgc tgaaggcgct cgcgggggat ctcatgaaaa tcgccaacga cattcgctgg
      301 ctcgcttccg gtccgcgatg cggtctcgga gaactcgtca ttcctgagaa tgaaccgggg
      361 agttcgatta tgccggggaa agtgaacccg acgcagtgcg aggccgttac gatggtggcc
      421 gtccaggtga tgggcaacga cgcggccgtc ggctttgccg ccagtcaggg gaactttgaa
      481 ctgaatgttt ttatgcccgt actcatctac aactttttgc agtcgtgccg tctgcttggc
      541 gacgttatgg actctttcga gcgcaacgcc gtcagaggca tggaagcgga cacgaagcgc
      601 ataaggacgt atgtgaacga atcgctgatg cttgtaacgg ccttgacgcc gcggatagga
      661 tacgacaaag ccgccgagat agcgaagaag gcgcacaagg agggaagcac gctcaaggag
      721 gcggccgttg ctctgcacta tctgacggag gatgaattcg acagcgccgt tgttccggaa
      781 aagatggtcg gaccgagata gaacgtcctc tcttttagaa agaaagagga gcttgtagta
      841 actggagaaa aaacaaaatc cggtgattgc aatacttccc tctctacaac cgacaaagtc
      901 gggaaagccg tcctccagcc catctagaaa atgcacaacg gccggcgaag gctcatccct
      961 tcaccgaccg ttgtacaatg agcttacaaa agatggaggg cgtcgatacg ctccgctatc
     1021 tgttccggta gcctttcagg gctttttcaa tcccttccat gccttctttt aacagcgctc
     1081 tgggcgtggc gatgttgatt ctggcaaacc cgtcgccgcc cgttccgaac cagtgtccgc
     1141 cgtcgagagc cactttgccc ttttccacga gcagcctttc cagctccggt ccggagagat
     1201 tcagcgagcg gcagtcgagc agcggtacat aggtcccctc ggggcgaagg agcgaaactt
     1261 ccggcagatg ctctgcgaga aatgtgtcga tgaagtccag attgccttca agatagcgca
     1321 acagctcgtc aagccatgcg tctcccttgg tgtatgccgc ttccagcgct gtggcgccga
     1381 agacgttgcc gccgtcgatg ttgacgaaat cgatgacgga ctggtagttg tcgcgcagtc
     1441 gtttcgaggg gatgacaacc gccgctgtgg caagtcccgc aatgttgaat gtcttgcttg
     1501 gcgcgactgt ggtgatggta ctctccctca tagcgtcgga gacggaagcg agcggagtat
     1561 gcttcgctcc tttatagagg atgtcgcagt gaatctcgtc ggagacgact acaacgccct
     1621 tcgcggcgca gatcgcgccg aacttttcga gttcttctct gctccagacg cgtcctaccg
     1681 ggttgtgggg agagcagagg aagacgagct tgac
//
LOCUS       DAOTIL010000667         1711 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminobacteriaceae bacterium isolate AMR_MDS_3026
            NODE_83355_length_1711_cov_1.681763, whole genome shotgun sequence.
ACCESSION   DAOTIL010000667 DAOTIL010000000
VERSION     DAOTIL010000667.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868050
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminobacteriaceae bacterium (wastewater metagenome)
  ORGANISM  Aminobacteriaceae bacterium
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae.
REFERENCE   1  (bases 1 to 1711)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1711)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:19:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,224
            CDSs (total)                      :: 3,198
            Genes (coding)                    :: 3,184
            CDSs (with protein)               :: 3,184
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1711
                     /organism="Aminobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_83355_length_1711_cov_1.681763"
                     /isolate="AMR_MDS_3026"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:3034609"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>1711
                     /locus_tag="P5201_11970"
     CDS             <1..>1711
                     /locus_tag="P5201_11970"
                     /inference="COORDINATES: protein motif:HMM:NF014442.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008170 - N-methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006306 - DNA methylation [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="N-6 DNA methylase"
                     /protein_id="HRV99306.1"
                     /translation="LERIFEDELFTDFALLFRLLHSSRWAAEGENPAESLLETYHQDS
                     LENGSRIREKLSEAVVDALETWAEGLLIHPDNSDFRTSLREGRTTPRQLYDLLLTLVY
                     RILFLLVIEERGLTHPPEAPGDLQEIYREYYSASMLRARCRSVWKEEERFSDLWTTLL
                     DCFSLHEDEEHASALGLRPLGGDLFSAESFAPLRPLSMDNRRFSEGMNRLDAYRDEKL
                     GVVRVNYAALNVEEFGSVYEGLLDLAAAVDDSDGVLRFRFVRGDDRGRSGSHYTPEEL
                     VRPLIEHALEPLIRNALEKPGAEERLLDLKICDDACGSGHILLNAARRVALEVARLRT
                     GADNPDPAAYRLALRDVIQRCIYGVDSNPQAVRLCKVALWLESHNPGKPLGFLDHRIK
                     CGDSLVGVAKVSELLDDIPDEAFDTRAGDDQEICAELRRRNAKERLEPRQIGIDWQTS
                     VGESLQEVVRSLRAVAAMDASTPEAAERKKEAYLRATDGENWRRLRTLADVKLAPFFL
                     PKRPETRVATRSLYRAFLDGTEDLDGHPVAFDASEAAKGRRFFHWFLEFADAMMVGGF
                     DCFL"
BASE COUNT          317 a          587 c          533 g          274 t
ORIGIN      
        1 acctcgaacg catcttcgag gacgagctgt tcaccgactt cgccctgctc ttccgcctgc
       61 tccatagcag cagatgggcc gccgaggggg agaaccccgc ggaatcgctc ctcgaaacgt
      121 accatcagga cagcctcgaa aacggcagcc gcatcaggga gaagctctcc gaagccgtcg
      181 tcgacgccct cgaaacatgg gcggaaggac tgctgatcca tccggacaac agcgacttcc
      241 gcacctccct gcgggagggg cgtacgaccc cgcgtcaact ctacgatctg ctgctcacgc
      301 tggtctacag gattttgttc cttctcgtca tcgaggagcg cggcctgacg caccctcccg
      361 aagcccccgg cgatctgcag gagatctacc gggagtacta ctcggcgagc atgctcagag
      421 cgcgatgccg ctccgtctgg aaggaggagg aacgcttttc cgacctctgg acgacgctgc
      481 tcgactgctt ctcgctccac gaggacgagg aacatgcctc cgcactgggg ctccgtccgc
      541 tcggcggcga tcttttctcg gcggagtcgt tcgccccgct ccgtccgctt tcgatggaca
      601 accgcagatt ctccgagggg atgaaccgcc tcgacgccta cagggacgag aagctgggcg
      661 tcgtgcgggt gaactacgcg gcgctcaacg tggaggagtt cggctccgtc tacgaggggc
      721 ttctcgacct cgcggcggcg gtcgacgatt cggacggcgt tcttcgcttc cgtttcgttc
      781 gcggagacga cagggggaga agcgggagcc attacacgcc tgaggaactg gtacgtcccc
      841 tgatcgagca cgccctcgaa cccctgatcc ggaacgccct cgaaaaaccc ggcgccgagg
      901 agcgactgct cgacctgaag atctgcgacg acgcctgcgg ctccggccac atcctgctca
      961 acgcggccag aagagtggct ctggaggtgg ctcgcctccg cacgggggcc gacaacccag
     1021 accctgcggc ctaccggctg gcgctgcgcg acgtcatcca gcgctgcatc tacggggtgg
     1081 actccaatcc gcaggcagtg cgcctctgca aggtggctct ctggctggaa tcgcacaacc
     1141 ccggcaagcc cctcggtttc ctcgaccacc gcatcaagtg cggcgacagc ctcgtcggcg
     1201 tcgcaaaggt ttccgagctt ctggacgaca tccccgacga ggcgttcgac acgagggcgg
     1261 gcgacgacca ggagatctgc gccgaactgc gccggaggaa cgcgaaagaa cgcctcgaac
     1321 cgagacagat tggaatagac tggcaaacga gcgtgggcga gagcctccag gaggtggtgc
     1381 gctccctccg cgccgtcgcc gcgatggacg cctccacgcc cgaggcggcc gagcgcaaga
     1441 aggaggccta cctccgcgca acggacggag agaactggcg ccgactgagg acgctagcag
     1501 acgtcaagct cgccccgttc ttcctcccca agcggccgga gacccgcgtc gccacccgga
     1561 gcctctaccg cgcctttctc gacggaacgg aagacctcga cggccacccc gtcgccttcg
     1621 acgcctcgga agcggcgaaa ggacggcgct tcttccactg gttcctcgag ttcgccgacg
     1681 cgatgatggt cggaggattc gactgctttc t
//
LOCUS       DAOTIL010000668         1708 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminobacteriaceae bacterium isolate AMR_MDS_3026
            NODE_83548_length_1708_cov_2.912886, whole genome shotgun sequence.
ACCESSION   DAOTIL010000668 DAOTIL010000000
VERSION     DAOTIL010000668.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868050
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminobacteriaceae bacterium (wastewater metagenome)
  ORGANISM  Aminobacteriaceae bacterium
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae.
REFERENCE   1  (bases 1 to 1708)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1708)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:19:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,224
            CDSs (total)                      :: 3,198
            Genes (coding)                    :: 3,184
            CDSs (with protein)               :: 3,184
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1708
                     /organism="Aminobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_83548_length_1708_cov_2.912886"
                     /isolate="AMR_MDS_3026"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:3034609"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            118..846
                     /locus_tag="P5201_11975"
     CDS             118..846
                     /locus_tag="P5201_11975"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013672.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HRV99307.1"
                     /translation="MHTSFLRTLCLILILLVSLNQNRGEASPPVYSGSRMVHTIAITF
                     DDGPHQILTPALLDTLDALDVKASFFLVGKMVDRYPALVCEIYARGHTVANHSYWHNN
                     SSLLSPEEFLDDLRRCSESIERNIPVRVRFFRPPGGNYTKNVLSLVEEAGMRTVLWDI
                     NSRDYTGVSAARMTARILSKVSPGSILLFHSGVSSTINALPEIVSSLRSKGFDFVTLD
                     EMLSGYSALLPHTTGSESSTLARK"
     gene            876..>1708
                     /locus_tag="P5201_11980"
     CDS             876..>1708
                     /locus_tag="P5201_11980"
                     /inference="COORDINATES: protein motif:HMM:NF012913.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparaginase"
                     /protein_id="HRV99308.1"
                     /translation="MASFLVISTGGTIASRAGSNGLVPSLPGEELLSFVPGVERFGEI
                     TVLDLLSKDSSNMSPEDWKMIAACLRAEEHNYNGFLVLHGTDTMAYSASALSFMLPGF
                     EKPVVLTGSMEPIGVPGSDAEDNIEGAFAFLSALFERKQRGVSIAFHNRLIHGPRSQK
                     ILSHESTAFSSINYPQLGCRENGAIVLEHTPVMHEKYPVNVHSLELETSIFLVTLFPG
                     FRSRYLSHLADAGPKAIVLEALGLGGVPYLGENLLPAIAMCRERNIPVIITTQCVYGG
                     V"
BASE COUNT          381 a          473 c          413 g          441 t
ORIGIN      
        1 attccagccc agcgttacga gaacgtggtc acaaagtccg ctggcgatcg ccattctgtc
       61 gtacacattt attctcggta ttgtgattct gcggaagata gaaggttgat tttgaatatg
      121 cacacttctt ttcttcgaac tctctgtctc attctcattc ttcttgtttc gttgaatcaa
      181 aaccggggcg aagcctcccc ccccgtctac tcgggaagca ggatggtcca tacaatcgca
      241 ataaccttcg acgacgggcc gcatcaaatt ctgacgccgg cactccttga cacgctcgat
      301 gcgcttgatg taaaagcaag ttttttcctt gtaggcaaga tggtggaccg ttacccggcg
      361 ctcgtgtgtg aaatatatgc acgcggacat accgtcgcaa atcactccta ctggcacaac
      421 aattcttcgc ttctctctcc tgaggaattc ctggatgatc ttcgacgatg cagcgagtcg
      481 atcgaacgaa acataccggt tcgggtacgt tttttccggc ctccgggagg caactatacg
      541 aaaaatgttc tttctctggt cgaagaagcc ggaatgcgaa ctgtgctctg ggatatcaac
      601 agccgggact acactggtgt ttcggccgcc aggatgacgg caagaattct ctcgaaagtg
      661 tctccgggtt ccatccttct ttttcattcc ggagtatcct cgacgatcaa cgcgcttccc
      721 gagatcgtct cctcgctccg aagcaagggc ttcgatttcg tcaccttgga cgaaatgctc
      781 tcggggtact ccgcgctcct ccctcacacc acgggaagcg agtcttcaac actcgcacgc
      841 aagtaatgta aacagtatcc aaaggagatg agaaaatggc atcgtttctt gtgatttcga
      901 ctggcggtac aatagcctca cgggcaggct ccaacggtct agtaccttcg ttgccggggg
      961 aggaacttct ttcgtttgtt cccggggttg aacgtttcgg ggagatcaca gttctcgatc
     1021 ttctttcaaa ggacagttca aacatgtccc ctgaagactg gaagatgatc gccgcatgtc
     1081 tcagagcgga ggagcacaat tacaatggat ttcttgtcct ccacgggacg gacaccatgg
     1141 cgtattctgc ctcggccctc tcttttatgc ttcccggctt cgaaaagccc gtagtactga
     1201 cgggttccat ggaacctatc ggggttccgg gctctgacgc cgaagacaac atagaaggcg
     1261 cttttgcatt tctctccgct ctctttgaac gaaaacaaag gggagtctcc atagcctttc
     1321 acaacaggct gatccacgga ccacgctccc agaagattct cagccatgaa tccactgctt
     1381 tttcaagtat caattacccc cagctcggat gccgggaaaa cggagcgatc gttttggaac
     1441 acactcctgt tatgcacgag aaatacccgg taaatgtaca ctctcttgaa ctggagacct
     1501 cgatttttct tgtaactctc tttcccggtt ttcgttcccg gtacctctct catcttgccg
     1561 acgccgggcc aaaggccata gttctcgaag cgctcggcct gggaggagtt ccgtatctgg
     1621 gtgagaatct gctcccggcc atcgctatgt gccgcgagcg aaacatcccg gtcattatta
     1681 cgacgcagtg cgtttacgga ggagtcga
//
LOCUS       DAOTIL010000669         1706 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminobacteriaceae bacterium isolate AMR_MDS_3026
            NODE_83687_length_1706_cov_3.390672, whole genome shotgun sequence.
ACCESSION   DAOTIL010000669 DAOTIL010000000
VERSION     DAOTIL010000669.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868050
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminobacteriaceae bacterium (wastewater metagenome)
  ORGANISM  Aminobacteriaceae bacterium
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae.
REFERENCE   1  (bases 1 to 1706)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1706)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:19:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,224
            CDSs (total)                      :: 3,198
            Genes (coding)                    :: 3,184
            CDSs (with protein)               :: 3,184
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1706
                     /organism="Aminobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_83687_length_1706_cov_3.390672"
                     /isolate="AMR_MDS_3026"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:3034609"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            40..192
                     /locus_tag="P5201_11985"
     CDS             40..192
                     /locus_tag="P5201_11985"
                     /inference="COORDINATES: protein motif:HMM:NF022894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3018 family protein"
                     /protein_id="HRV99309.1"
                     /translation="MSRVLLLVEAQTERGIAVACRRQSRMLGDDPQERELLDFIETAT
                     DTESWE"
     gene            279..551
                     /locus_tag="P5201_11990"
     CDS             279..551
                     /locus_tag="P5201_11990"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRV99310.1"
                     /translation="MKMVSVQEIKKNPAVLYSGPGYRVLTSNNRPKALCFPLDELDDI
                     EEIVASFRQAQAMRAVERVRSFAVEKGLDGMTMEDVDEEIRRARNG"
     gene            779..1021
                     /locus_tag="P5201_11995"
     CDS             779..1021
                     /locus_tag="P5201_11995"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system Phd/YefM family
                     antitoxin"
                     /protein_id="HRV99311.1"
                     /translation="MPVALGNSLREAIRSARSGGRTVLYRKGKPAFAIVPAEDAEYMQ
                     KLEDELDNRLADEALAEDSEGVPIEILFQRYGIERE"
     gene            1002..1274
                     /locus_tag="P5201_12000"
     CDS             1002..1274
                     /locus_tag="P5201_12000"
                     /inference="COORDINATES: protein motif:HMM:NF016876.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008657 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) inhibitor activity
                     [Evidence IEA];
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA];
                     GO_process: GO:0030541 - plasmid partitioning [Evidence
                     IEA];
                     GO_process: GO:0051290 - protein heterotetramerization
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HRV99312.1"
                     /translation="MESKGNKQFEIVFHRQAEKTLDGLTGSLRKRLLIAIDRLSQEPR
                     PQGCILLKIERRYRIRVGDWRVVYEIHDGILRVLVIRIAHRREVYD"
     gene            complement(1479..>1706)
                     /locus_tag="P5201_12005"
     CDS             complement(1479..>1706)
                     /locus_tag="P5201_12005"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRV99313.1"
                     /translation="GQGLVGKTIVQFVLQLLHELRILRRYDSECRKITVKKRPSEEGR
                     TFSLRISEAPCPSKNSGPGRSPCTIRRVPGL"
BASE COUNT          428 a          414 c          498 g          366 t
ORIGIN      
        1 aactgtggca gaagaacatc ttcgacggag gcccttcgca tgagtagagt tctgctgctt
       61 gtggaggcac agacggaacg cggcatcgcg gtggcgtgcc ggagacagtc gcgcatgctc
      121 ggcgacgatc cgcaggagcg cgagctgctt gatttcatcg aaaccgcaac ggatacggag
      181 agttgggagt gaaaacgaga tacgccccct gtttcgagat acaatagggg tgctaatgta
      241 gcataatata tcgaaggatt aaacggaggt ggttgatcgt gaagatggtg tccgttcagg
      301 agatcaaaaa gaatccggcg gttctttaca gcggccccgg ataccgtgtg ctgacatcca
      361 ataatcgccc gaaggcgctc tgtttcccct tggatgagtt ggacgacatc gaggagatcg
      421 tggcgagctt tcggcaagcc caagcgatga gggcggtgga acgagtgcgc tccttcgctg
      481 tcgagaaggg actcgacgga atgactatgg aagatgtcga cgaggagata cgtcgcgccc
      541 gtaacggtta gctcagtctc atgctctaag aagatatgct ccatcctttt cgaactcatt
      601 gtgcccctga tcaactccac cgcaaacgat cgacttttcc tggaaagctc catgaaaagc
      661 gggaagacca ttcgcagacc cctccgctct tcagcccaat tcataccggc ctgttcatgc
      721 tatacttatt ccaattacca agccaagaac caatttcact ttccggggag gtctttctat
      781 gcccgttgct ctcggaaaca gtctcaggga ggccattcgt tcggctcgct ccggggggag
      841 aacagtgttg tatcgcaaag ggaaaccggc attcgccatc gtaccggcgg aggatgccga
      901 gtacatgcaa aagctggagg acgaactgga caatcgtctt gccgacgagg ccttggccga
      961 ggattcggaa ggcgttccga tagagattct ttttcagcga tatggaatcg aaagggaata
     1021 agcaatttga gatagtcttc cataggcaag cggaaaaaac tttagacggt ctgaccggga
     1081 gtctaaggaa aagactgctt atagctatag accgtctctc ccaagaacct cgtcctcaag
     1141 gatgcatctt gctcaagatc gaacgacgct atcgaatccg agttggagac tggcgtgtcg
     1201 tctacgaaat tcatgatggc attttgcgtg ttcttgtaat acgtatagcc cacaggcgag
     1261 aagtgtacga ctgaactgaa aaaagccatc ttgggaaaaa cactccgcgc ggatgttcct
     1321 tgacggtaag tccgccgaaa gaatgaagga agaggagttg cacgaagcag ggtcttgaaa
     1381 tgacgaggga gaaggtaacg cttgccgaag agcatatcga tctcgagtcc gttctgacgg
     1441 acggatgacc gttcccgtgc cggaagacgc gccccggatc agagtccggg aactcgacgg
     1501 atggtgcagg gcgaacggcc gggacccgag ttctttgacg gacaaggagc ttcggagatt
     1561 cgtaagctga acgtacgccc ctcttcggag gggcgttttt ttacggtgat tttccggcat
     1621 tcactatcgt accggcggag gatacggagt tcatgcaaaa gctggaggac gaactggaca
     1681 atcgtcttgc cgacgaggcc ttggcc
//
LOCUS       DAOTIL010000670         1700 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminobacteriaceae bacterium isolate AMR_MDS_3026
            NODE_84086_length_1700_cov_3.908207, whole genome shotgun sequence.
ACCESSION   DAOTIL010000670 DAOTIL010000000
VERSION     DAOTIL010000670.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868050
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminobacteriaceae bacterium (wastewater metagenome)
  ORGANISM  Aminobacteriaceae bacterium
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae.
REFERENCE   1  (bases 1 to 1700)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1700)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:19:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,224
            CDSs (total)                      :: 3,198
            Genes (coding)                    :: 3,184
            CDSs (with protein)               :: 3,184
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1700
                     /organism="Aminobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_84086_length_1700_cov_3.908207"
                     /isolate="AMR_MDS_3026"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:3034609"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            258..1511
                     /locus_tag="P5201_12010"
     CDS             258..1511
                     /locus_tag="P5201_12010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008709814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HRV99314.1"
                     /translation="MYIKRAVEDTVRKTAATFPVLLVTGPRQVGKSTLLERLAEPERR
                     IVTLDDPDVRYLAGNDPALFMQRFTPPVLIDEIQYAPELLPYIKMAVDRSGRSGDFWL
                     TGSQSFVMMKNVGETLAGRVGIIRLSGLSTSEIRGIPSKPFTTDPKSLLERAEEVQRL
                     ELNALYERIFKGSMPRLYADERVDWETYYRSYIDTYLKRDIKDLTQVADEMAFYNFMT
                     VVAARTSRPVVYEELAGECGISAPTAKKWLSVLVSSDIVAIVQPYFNNILKRVTKMPA
                     LHFLDTGLCAYLLKWGNPEALERGAMSGAFFESYVFSEVYKSYLNAGREPPLFYYRDK
                     EKREIDLLLYENGTLSPVEIKKSASPGKDAVKHFRVLEPLSDPENSGEPAGMKVGIGN
                     GAVVCMANDLLPVDSKNWYVPAWMI"
BASE COUNT          377 a          453 c          493 g          377 t
ORIGIN      
        1 gtaattttcg ccgctacccg ccgcagggaa cggaaactcg aactctccgg cgcgccgctg
       61 tagtccgtgg ttgcgtaaaa aaaactctaa aaaccgtcgc ctcatcatga ttgcgcttcc
      121 catagagttg tttgcctgct gtgggagcgg agttccggca aaacgggctt gcgtcttcac
      181 agtaatttga ctataataaa tatgtgtttt agtttggtcg gataattggc tccgctcccg
      241 gaaacgaggt gaggtcagtg tatatcaaaa gagccgtcga ggataccgtc aggaagacgg
      301 cggccacatt cccggtgctg cttgtgaccg gtccgcgcca ggtcggcaag tcgacgcttc
      361 tcgaacgcct tgcggagcct gaacgcagga tcgtgacgtt ggacgatcct gacgtcaggt
      421 atcttgccgg aaacgaccct gcgcttttta tgcagcgatt cacgcctccg gtgctgatcg
      481 acgaaattca gtacgccccg gagctgctgc cctatatcaa gatggctgtg gaccgttccg
      541 gacggagcgg cgacttctgg cttaccggtt cccaatcctt cgtgatgatg aaaaatgtcg
      601 gcgaaacttt ggccgggcgc gtcgggatca tcaggctttc ggggctttcg accagcgaga
      661 ttcggggaat tccctcgaag cccttcacga cggatccgaa aagccttttg gagcgcgccg
      721 aagaagtaca aaggcttgag ttgaacgcgc tgtacgagcg catattcaag ggctccatgc
      781 cgaggttgta tgcggatgag agagtggatt gggagactta ttaccgttcc tacatcgaca
      841 cgtatctcaa acgcgatata aaggatctga cacaggtggc ggacgagatg gccttctata
      901 acttcatgac cgttgtcgcc gccagaacat ccaggccggt cgtctacgaa gaactggcag
      961 gcgagtgcgg catttccgcg ccgaccgcga agaaatggct ctccgtgctc gtctcatccg
     1021 atatcgtggc gattgtgcag ccgtacttca acaacatatt aaagcgagtg accaaaatgc
     1081 ccgcgcttca ctttctggat acggggctct gtgcgtattt gctgaagtgg ggtaatcccg
     1141 aagcgctgga gagaggggcc atgtccggcg cttttttcga gagctacgtc ttctccgaag
     1201 tgtacaagag ctatctgaac gccggaagag agccccccct cttttactac cgcgacaagg
     1261 aaaaacggga gatcgatctg ttgctgtatg aaaacggaac gctttctccc gtggagatca
     1321 agaagtcggc atcccccggg aaagacgctg tgaagcactt ccgggtgctg gagcccctga
     1381 gcgaccctga aaattccggc gaaccggccg gaatgaaagt cgggataggg aacggggctg
     1441 ttgtctgcat ggccaacgac ctgctgccgg tcgattcgaa aaactggtat gtcccggcct
     1501 ggatgatatg acgagggaac tccggcggtg aaagaagcgt tctctcctcc gccggagttc
     1561 cctttgcttc gtgcaaagcg atctatcaga ggaacagcag ctctttgcgt gcggcttcag
     1621 ggttcaggat atgcacaagt tcctcgatct cctccaggtg cgcgcgcacc tctccggcgg
     1681 aaatagacgg cgttccgtac
//
LOCUS       DAOTIL010000671         1696 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminobacteriaceae bacterium isolate AMR_MDS_3026
            NODE_84367_length_1696_cov_3.148080, whole genome shotgun sequence.
ACCESSION   DAOTIL010000671 DAOTIL010000000
VERSION     DAOTIL010000671.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868050
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminobacteriaceae bacterium (wastewater metagenome)
  ORGANISM  Aminobacteriaceae bacterium
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae.
REFERENCE   1  (bases 1 to 1696)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1696)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:19:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,224
            CDSs (total)                      :: 3,198
            Genes (coding)                    :: 3,184
            CDSs (with protein)               :: 3,184
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1696
                     /organism="Aminobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_84367_length_1696_cov_3.148080"
                     /isolate="AMR_MDS_3026"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:3034609"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..900
                     /locus_tag="P5201_12015"
     CDS             <1..900
                     /locus_tag="P5201_12015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014162675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent enzyme"
                     /protein_id="HRV99315.1"
                     /translation="SAGELLPEVIADILGGLLQDDSLAGKQHPLLKEATEDLNIVPRK
                     PMLCPGCPHRASFFSIRQALPNAINPSDIGCYTLGVNQKAVDAVVDMGASVTMASGFY
                     LAHKAAGIDRPVVTTIGDSTFFHMGLPGLASAVYNRHAFVLAILDNSITAMTGGQANP
                     AVGTKLRKGDEGRKVDIEGACRGCGVEYVKTIAAYDVALGKAAVKEAWEYAKSKGEPA
                     VLIFRHPCMLLRPEQPSIPVKVDQEKCIGCKFCINFFNCPGLVFSEETGKAYIDERFC
                     VSCGVCVSVCPHGAIVASSEEAE"
     gene            902..1462
                     /locus_tag="P5201_12020"
     CDS             902..1462
                     /locus_tag="P5201_12020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008520886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016903 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoacid:acceptor oxidoreductase family
                     protein"
                     /protein_id="HRV99316.1"
                     /translation="MQYVIVGIGGQGILFASKALGAIAMNNGLHVMGSEVHGMAQRGG
                     SVVSHFKTGEYVSPLVRAGEADLLLAFDQNEGVRNLHFLREGGAFVVNVHDASAFANP
                     KLDAFLKERRVSVFALEGYRLLKEHMGGNFLFLNVLLMGGMCGAGVGAPSFDETASAV
                     KALSPAKFEDANMKVFKLGYETIANS"
BASE COUNT          315 a          502 c          525 g          354 t
ORIGIN      
        1 agcgcgggcg agctgcttcc ggaagtgatc gccgatattc tcggagggct cctccaggac
       61 gattcccttg cgggaaagca gcatcctctt ctgaaagagg cgacggagga tctgaatatc
      121 gtgccgcgca agccgatgct ctgtcccggc tgcccgcaca gggcgagctt cttctccatc
      181 cgccaggcgc ttccgaacgc gatcaaccct tcggacatcg gctgctatac gctcggagtc
      241 aaccagaagg ccgtggacgc ggtcgtcgat atgggcgctt ccgtcacaat ggcctccggg
      301 ttctaccttg cccacaaggc cgccggaatc gaccgtcccg tcgtcaccac catcggagac
      361 tccaccttct tccacatggg gctgcccgga ctggcgagcg ccgtctacaa tcgccacgct
      421 ttcgtgctgg ccatccttga caacagcatc accgccatga cgggcggaca ggcgaacccc
      481 gccgtcggaa cgaagctccg caagggcgac gaaggacgga aggtcgatat cgaaggcgcc
      541 tgccggggct gcggcgtgga gtatgtgaag actatcgccg cctacgacgt agcgcttggc
      601 aaggctgctg tcaaagaggc gtgggagtac gcgaaatcaa aaggggaacc agccgtgctg
      661 atcttccgtc acccgtgcat gctgctccgt ccggagcagc cgtcgatccc ggtgaaggtc
      721 gaccaggaga agtgcatcgg ctgcaagttc tgcatcaact tcttcaactg cccgggtctt
      781 gtcttctccg aggagacggg gaaggcgtac atcgacgaac ggttctgcgt gtcatgcggc
      841 gtctgcgtgt cggtctgccc gcacggagcg attgtcgcgt cttcggagga ggctgagtga
      901 gatgcagtac gtcatagtcg gtatcggcgg tcaaggaatt cttttcgcca gcaaggcgct
      961 tggcgccatc gccatgaaca acggcctgca cgtcatgggg agcgaagtgc acggcatggc
     1021 ccagagaggc ggatcggtgg tcagccactt caagaccggg gaatacgtca gtccgcttgt
     1081 cagagcgggg gaggcggatc tgctcctcgc gttcgaccag aacgagggcg tgcgcaacct
     1141 gcattttctc cgcgaagggg gcgctttcgt ggtaaacgtc cacgacgcgt cggctttcgc
     1201 aaatccgaaa ctcgacgcat ttctgaagga gcggcgggtt tccgtctttg ccctcgaagg
     1261 ctatcgtctt ttgaaagagc acatgggcgg gaatttcctc ttcctgaatg tgctcctcat
     1321 gggcgggatg tgcggcgccg gcgtgggagc tccttccttc gacgagacgg cgtcggcggt
     1381 caaggcgctt tcgccggcca agttcgagga cgccaatatg aaagtattca agctggggta
     1441 tgaaacgatc gccaactcct gaatctgtcc gctgtgccgc gagaggaacc cttttccggg
     1501 gttcccttcg cagtgcgttc tccctcatcg aactgcttgt cgtcatactc attcttggag
     1561 ttctttacgg gggcgtcgcg ctggcgccct cttccatttt cttggactcc tcatcccctg
     1621 agagcgttcg atgcgaaaca gagcgtgctg cctcatggtt gcaacgggtc ttttataaag
     1681 cgcttttgtc cgggag
//
LOCUS       DAOTIL010000672         1694 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminobacteriaceae bacterium isolate AMR_MDS_3026
            NODE_84497_length_1694_cov_5.006101, whole genome shotgun sequence.
ACCESSION   DAOTIL010000672 DAOTIL010000000
VERSION     DAOTIL010000672.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868050
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminobacteriaceae bacterium (wastewater metagenome)
  ORGANISM  Aminobacteriaceae bacterium
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae.
REFERENCE   1  (bases 1 to 1694)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1694)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:19:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,224
            CDSs (total)                      :: 3,198
            Genes (coding)                    :: 3,184
            CDSs (with protein)               :: 3,184
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1694
                     /organism="Aminobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_84497_length_1694_cov_5.006101"
                     /isolate="AMR_MDS_3026"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:3034609"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..375)
                     /locus_tag="P5201_12025"
     CDS             complement(<1..375)
                     /locus_tag="P5201_12025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013048438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PSP1 C-terminal domain-containing protein"
                     /protein_id="HRV99317.1"
                     /translation="MSIHLAIFGKPRYLGLLNIEAPFPSKGTWLVVETMRGSELALLG
                     GPLSDEQEARYRASCNDDASDGQVKGGEPILQEISLVRTALDEDLAAAELEREEENEV
                     LIRARKLLRNHNLSMKLVDVEYL"
     gene            complement(347..1000)
                     /locus_tag="P5201_12030"
     CDS             complement(347..1000)
                     /locus_tag="P5201_12030"
                     /inference="COORDINATES: protein motif:HMM:NF024575.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRV99318.1"
                     /translation="MHAALGIVRVLLCSNGSGDDDCRVCSAWSGERHPDLLYCGAPSK
                     PPSIDECREIIDAMAYRPVVSSKRVAVVFSADRMMLPAANSLLKLAEEPPEYVHMLFL
                     LTDEKLFLPTLRSRSWSVSLPVPRSGESMPPPQTEEEWIRWIEKNAKSEVDELIVLFA
                     PWISYEVERGAYDKAAALERIRLLIQTKRLSRTMVLDLIVLALKEGIVFEHSFGDLW"
     gene            complement(1132..>1694)
                     /locus_tag="P5201_12035"
     CDS             complement(1132..>1694)
                     /locus_tag="P5201_12035"
                     /inference="COORDINATES: protein motif:HMM:NF015822.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF327 family protein"
                     /protein_id="HRV99319.1"
                     /translation="GSRGLFFPGQERASKDLHGRGFFRTASSCVKNSLSCGGVFVPLR
                     VKKTSKETLSGGEFSKTSKRSGQLPSRESTASVFSASLEDAEIRDLLDRLDIIGRKLS
                     IFPAEALLIQYRKLVSELVRRAVAGLRVRRDMKWRRGDKNFFVIVEKTDSMLDELESV
                     FAREGERTRMLQLMEEIKGCLFSLLF"
BASE COUNT          457 a          459 c          380 g          398 t
ORIGIN      
        1 aagatattca acgtcaacaa gcttcataga gaggttatga ttacgaagca gttttctcgc
       61 cctgataagg acttcgtttt cttcttcccg ttcgagttca gcagctgcaa gatcttcatc
      121 cagcgccgtc cgtacgagcg agatttcctg gagaatcggt tcgcctccct tgacctgacc
      181 gtcagaggca tcgtcattac aagaagcccg gtaacgcgct tcttgttcgt ccgagagggg
      241 accgccaaga agagccagtt cagaacctct catcgtttcg acaacgagcc aggttccctt
      301 ggaagggaaa ggggcttcta tgttgagaag cccaagatat cgaggtttac caaagatcgc
      361 caaatgaatg ctcaaaaaca ataccctcct tgagagccaa tacaataagg tcgagaacca
      421 tggttcggga aagacgtttc gtctggatca aaaggcgtat tcgctctagc gctgccgcct
      481 tgtcatacgc tccccgttct acttcatagg atatccatgg agcaaagagc acaatcagct
      541 cgtcgacttc agacttggca tttttttcaa tccaccgaat ccactcttcc tctgtttgcg
      601 gcggcggcat gctctcaccc gaacgaggca caggaagaga aacactccac gaacggcttc
      661 tcagtgtggg cagaaagagt ttctcgtccg taagtaaaaa aagcatatga acatactccg
      721 gcggttcttc tgccagtttt aacagactgt ttgccgcagg aagcatcatt ctgtctgcag
      781 aaaaaacaac tgcaactctt tttgatgata ccaccggacg atacgccatt gcatcgatta
      841 tctcacgaca ctcgtcaata gaaggaggtt ttgagggagc gccgcaatac aaaagatccg
      901 gatgcctctc gccggaccag gctgaacaga ccctgcaatc atcatctccc gaaccgttac
      961 tacagagcag cacgcgaaca attccgagcg ctgcgtgcat atggaggttc tcgtcgatca
     1021 ctactgcgca cgagtgaggg aaggctcctc ttgatgccaa aaacagcaaa cgtttccatg
     1081 ccgaagagtc cttccatgaa gcgccgcgct gctcggagat tccttccgtg ttcagaacag
     1141 aagagaaaac agacatccct tgatctcctc cattaattgc aacatcctgg ttcgctctcc
     1201 ttcccgagcg aaaacagatt ccaattcatc aagcatggag tctgtctttt ccacaataac
     1261 gaagaaattc ttatcccctc tacgccattt catatcccgc cgtacacgta aaccggctac
     1321 tgcccgtcgc accagctcgg aaacaagttt acggtactga ataaggagcg cctccgcagg
     1381 gaaaatagaa agttttcttc cgataatgtc aaggcgatcc aaaagatcgc ggatttcagc
     1441 atcctcaaga gaagccgaaa aaacggaagc agtcgattca cgagaaggca actgcccgct
     1501 tcttttcgat gttttggaaa attctcctcc gcttagtgtt tctttggaag ttttttttac
     1561 cctcaacggg acaaagacac ctccacaaga gagagaattc ttgacgcaag agcttgcggt
     1621 tctgaagaag cctcgaccgt gcaaatcctt tgaggctctc tcttggccag ggaagaaaag
     1681 ccctcgcgaa ccct
//
LOCUS       DAOTIL010000673         1693 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminobacteriaceae bacterium isolate AMR_MDS_3026
            NODE_84580_length_1693_cov_3.125763, whole genome shotgun sequence.
ACCESSION   DAOTIL010000673 DAOTIL010000000
VERSION     DAOTIL010000673.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868050
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminobacteriaceae bacterium (wastewater metagenome)
  ORGANISM  Aminobacteriaceae bacterium
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae.
REFERENCE   1  (bases 1 to 1693)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1693)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:19:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,224
            CDSs (total)                      :: 3,198
            Genes (coding)                    :: 3,184
            CDSs (with protein)               :: 3,184
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1693
                     /organism="Aminobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_84580_length_1693_cov_3.125763"
                     /isolate="AMR_MDS_3026"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:3034609"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1677
                     /locus_tag="P5201_12040"
     CDS             <1..1677
                     /locus_tag="P5201_12040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009200333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter fused permease subunit"
                     /protein_id="HRV99320.1"
                     /translation="VPLYDWILAFISTLPGIYNMINYTHIIERIAQVDPLTTTQLVLG
                     TLLLVILLEATRRVVGVPLTIIAAFFAGYMYFGHHMPGIMKGLSSTFEEVIEHIYLTG
                     EGIFSVPLGVSAAFVMIFLIFGGFLEKSGVGEYFMHLAEAFTGTQAGGPAKIAVVSSA
                     LFGSISGSAVANVYGTGTFTIPLMKRIGYPAHFAGAVEAVASAGGQIMPPIMGAGAFI
                     MASFLGRPYSEIMIAATLPAILYYGAVIFMVHLGALKNGLQGLEPDELPSKKEVLKKL
                     YFILPIIGLVYMLLDGYTPMWAATIGIGLTWAISMLNPGKRMTPIDAIDAIYTGAKNI
                     PMVCIACATAGIVVGSVGLTGFGFKFVGFIFAFAKDTPFVALLLVMFVSLILGMGLPT
                     TGAYILAAALGVPALAKLGFPTLSAHLFVFYFAIVSNITPPVALAAYAASSIAGSSPN
                     KTGFTAMRLGILAFIVPFAFCYDTGLLLNAGFFNNITALAGGVAATFALGYAMIGFTR
                     RPLALWQRAFFCIAGILCLSPLEISKAAGVGLLLLGLFFWRNPKPEHRIA"
BASE COUNT          293 a          586 c          432 g          382 t
ORIGIN      
        1 gtcccgctct acgactggat tcttgcattt atctcgacgc tgcccggcat ctacaacatg
       61 atcaactata cccacatcat cgagcgcatc gcccaggtgg atcccctgac cacaacacag
      121 cttgtcctgg gcacactgct gcttgtcatc cttctcgaag ccacccggcg cgtcgtcggc
      181 gttccgctga caatcatcgc ggcctttttt gcgggctaca tgtacttcgg ccaccacatg
      241 cccggcataa tgaagggcct ctcttctaca ttcgaagagg tgatcgagca catctacctt
      301 accggcgaag gaattttttc ggtaccgctc ggtgtctccg cggcgttcgt catgatcttc
      361 ctcatcttcg gcggcttcct tgaaaaaagc ggcgtcggcg agtacttcat gcaccttgcc
      421 gaagccttca caggcacgca ggcaggcggc ccggcgaaga tcgccgtcgt cagctcggcg
      481 ctcttcggct ccatttcggg ctccgcagtc gccaacgttt acggaacggg tactttcacc
      541 atccccctga tgaagcgcat cggctacccc gcgcacttcg ccggagccgt agaggccgtc
      601 gccagcgccg gagggcagat catgcctccg ataatgggag ccggcgcctt catcatggca
      661 tccttcctcg gccgccccta cagcgagatc atgatcgccg caacactccc cgcaatactc
      721 tactacggcg ccgtcatctt catggtgcat ctcggcgcac tcaagaacgg actgcagggc
      781 ctggaaccgg atgaactccc ctccaaaaaa gaagtgctga agaagctcta cttcatcctt
      841 cccatcatcg gcctcgtcta catgctgctc gacgggtata cgccgatgtg ggcggcaact
      901 atcggcatcg ggctcacatg ggcgatctcc atgctcaacc ccggtaagcg tatgaccccc
      961 atcgacgcca tcgacgcgat ctacaccggc gccaagaaca ttcccatggt gtgcatcgcg
     1021 tgcgccactg cgggtatcgt cgtcggctcg gtcggtctca ccggcttcgg cttcaagttc
     1081 gtcgggttca ttttcgcctt tgccaaggac acccccttcg tcgcgttgct gcttgtcatg
     1141 ttcgtctcgc tgatcctcgg catggggctt ccgacgacag gcgcctacat cctcgccgcc
     1201 gccctcggcg ttcccgccct ggcgaagctc ggtttcccga cgctctccgc acacctcttc
     1261 gtcttctact tcgccatcgt ctcaaacatc acccctccgg ttgcgcttgc cgcatacgcc
     1321 gcaagctcca tcgccgggtc ttcccccaat aaaacgggct ttacggcaat gcgcctcggt
     1381 attctcgcct tcatcgtccc cttcgctttt tgttacgaca cgggactgct gttgaacgcc
     1441 gggttcttca acaacatcac tgcgctcgcg ggaggcgtcg ccgctacctt cgccctgggg
     1501 tacgccatga tcggcttcac gaggcgaccg ctcgccctct ggcagcgcgc gttcttctgc
     1561 attgccggca ttctttgctt aagcccgctc gagatctcca aagcggcggg agtcggtctt
     1621 ctgttgctgg ggctcttctt ctggagaaac ccgaagccgg aacacaggat cgcgtaatta
     1681 cgaaggggcc gct
//
LOCUS       DAOTIL010000674         1692 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminobacteriaceae bacterium isolate AMR_MDS_3026
            NODE_84642_length_1692_cov_4.299939, whole genome shotgun sequence.
ACCESSION   DAOTIL010000674 DAOTIL010000000
VERSION     DAOTIL010000674.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868050
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminobacteriaceae bacterium (wastewater metagenome)
  ORGANISM  Aminobacteriaceae bacterium
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae.
REFERENCE   1  (bases 1 to 1692)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1692)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:19:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,224
            CDSs (total)                      :: 3,198
            Genes (coding)                    :: 3,184
            CDSs (with protein)               :: 3,184
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1692
                     /organism="Aminobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_84642_length_1692_cov_4.299939"
                     /isolate="AMR_MDS_3026"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:3034609"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..946)
                     /locus_tag="P5201_12045"
     CDS             complement(<1..946)
                     /locus_tag="P5201_12045"
                     /inference="COORDINATES: protein motif:HMM:NF013482.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2Fe-2S iron-sulfur cluster-binding protein"
                     /protein_id="HRV99321.1"
                     /translation="MAFRKMWLKINGANRIFICDPEKDSLADVLRRLGLTSVKIGCGT
                     GVCGSCSVILDGALVRSCTRKIGKIPEYSEVITLEGIGTPDHLHPLQVAFMKLGAVQC
                     GFCTPGFIVSSYALFLENKNPTRKEVRTWFQKHKNVCRCTGYKQIVDAVMEAAKVVRG
                     EARIDEIGPSYSTEDGYYGKPLVRPTALAKVCGLADYGDDQELKMPAETLHVAMVQPR
                     VAHHAKILEIDISEAEKMPGVAKIILAADIRAAGGTNIMAEGQMHERTTVMAPSREVL
                     NEKKIFRYGDVVALAVADTKDHARAAAAKVKVTIEQLPE"
     gene            complement(977..1300)
                     /locus_tag="P5201_12050"
     CDS             complement(977..1300)
                     /locus_tag="P5201_12050"
                     /inference="COORDINATES: protein motif:HMM:NF018563.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine/purine nucleoside phosphorylase"
                     /protein_id="HRV99322.1"
                     /translation="MDVFKAVDMAVKGNIYEGGKVTSRTFWTADGERKTVGIMLPGSY
                     SFSTSTQEKMEMTNGIVEVRLDPDGEWSRYKEGEFFMVPAGVTFEIRCAGVAEYVCSY
                     LNESK"
BASE COUNT          309 a          503 c          485 g          395 t
ORIGIN      
        1 actcggggag ctgttcgatc gtcaccttta ctttcgcggc tgcggcgcgg gcatggtctt
       61 tcgtgtccgc cacggcgagg gcgacgacgt cgccgtaccg gaagatcttc ttctcgttga
      121 gcacttcgcg gctcggcgcc ataactgtgg tgcgctcgtg catctgtccc tcggccatga
      181 tgttcgtccc gcccgcggcc ctgatgtcgg cggcaagaat gatcttcgcc actccgggca
      241 tcttttcggc ctcggagatg tcgatttcga ggatcttcgc atggtgcgcg acgcggggct
      301 gcaccatggc gacgtggagc gtctccgcgg gcatcttcag ctcctgatcg tctccgtagt
      361 ccgcgaggcc gcacactttt gcaagcgctg taggacggac gagaggcttt ccgtagtacc
      421 cgtcctcggt cgagtaggag gggccgatct cgtcgattct tgcctcgccg cggacgacct
      481 tcgccgcttc catgaccgcg tcgacgatct gcttgtagcc tgtgcagcgg cagacgttct
      541 tatgcttctg gaaccatgtc cggacctctt tgcgcgtcgg gtttttgttc tcgagaaaga
      601 gcgcgtagga cgagacgatg aatcccggag tacagaagcc gcactgtacc gcgcccagct
      661 tcatgaaggc gacctgcaga gggtgcaggt gatccggcgt tccgattcct tcgagcgtga
      721 tgacttcact gtactcggga atcttgccga tctttctggt gcaggaacgc acgagcgctc
      781 cgtcgaggat cacggaacag gagccgcaga cgccggtccc gcatccgatc ttcacgctcg
      841 tcaggccgag ccggcgaagc acatccgcca gagagtcctt ctcgggatcg cagatgaata
      901 tccggttcgc gccgttgatc ttgagccaca tctttcgaaa agccacagcg gtaccccctt
      961 tatggtttct tccgtactac ttgctctcat tcagataact gcacacgtat tcggcgaccc
     1021 cggcgcagcg gatctcgaac gtgacacccg ccggaaccat gaagaactct ccctctttgt
     1081 acctggacca ttctccgtcc ggatcgagcc ggacctcgac gatgccgttc gtcatctcca
     1141 tcttctcctg cgtcgaggtt gagaacgaat agctccccgg cagcatgatc ccgaccgtct
     1201 tccgttcccc gtcggctgtc cagaaggttc tgctggtgac cttcccgccc tcgtagatgt
     1261 tccctttcac cgccatgtcg accgccttga acacatccac atgaaccagc tcctctcgga
     1321 taggcgcgcc tccggaagag aggggcgctt catagtccac tcagtatatg aagagtacag
     1381 ggagatatgc aatattttga tatttgtgac tacttgtgtt tctttttggt cggttttgct
     1441 cacttatgac aacgcgcgcc ttcccccgta gtgtgtccgg cgccaaggcg gggaggcgtt
     1501 ccggagaggg gtgtgtaaag agacggatgc gttcagaaaa atgttttttt gttggcaatg
     1561 cagagggaaa tggagtataa tggccccggc gaggagatgt ttgtgaaaca agcaacatcc
     1621 ttgcgaaggg ccggcgaaat ggcccttgaa tagagaagcc tccgagccgt atttcctaac
     1681 gggtgtgcac cc
//
LOCUS       DAOTIL010000675         1691 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminobacteriaceae bacterium isolate AMR_MDS_3026
            NODE_84722_length_1691_cov_3.350244, whole genome shotgun sequence.
ACCESSION   DAOTIL010000675 DAOTIL010000000
VERSION     DAOTIL010000675.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868050
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminobacteriaceae bacterium (wastewater metagenome)
  ORGANISM  Aminobacteriaceae bacterium
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae.
REFERENCE   1  (bases 1 to 1691)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1691)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:19:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,224
            CDSs (total)                      :: 3,198
            Genes (coding)                    :: 3,184
            CDSs (with protein)               :: 3,184
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1691
                     /organism="Aminobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_84722_length_1691_cov_3.350244"
                     /isolate="AMR_MDS_3026"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:3034609"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..304)
                     /locus_tag="P5201_12055"
     CDS             complement(<1..304)
                     /locus_tag="P5201_12055"
                     /inference="COORDINATES: protein motif:HMM:NF022128.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006576 - biogenic amine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EutP/PduV family microcompartment system
                     protein"
                     /protein_id="HRV99323.1"
                     /translation="MRHAPGHSMKRVMIVGPTGSGKTSLLKALGIWQQDVRKTEAVSF
                     GDDAVDTPGEFFDIPHFYHALIMTSVKAGVILLDADPLKRSRHSSRFAHALRAPVIG"
     gene            complement(383..727)
                     /locus_tag="P5201_12060"
     CDS             complement(383..727)
                     /locus_tag="P5201_12060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005661357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BMC domain-containing protein"
                     /protein_id="HRV99324.1"
                     /translation="MTEETKARVVQEYVPGKQVTLAHVICNPRPELCSLIGVEKSGAI
                     GIMTITPGEGAIIAADIASKSGDIAMDFIDRFTGCVLFTGDVAAVESSLEGTVRTLAT
                     VLGFTPAPLTRT"
     gene            1100..>1691
                     /locus_tag="P5201_12065"
     CDS             1100..>1691
                     /locus_tag="P5201_12065"
                     /inference="COORDINATES: protein motif:HMM:NF024571.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HRV99325.1"
                     /translation="MLAFTDEKLLNLLIQYNPWWRGVPISEKTSPPKRRIAYYEAEKM
                     LLHPSLRRFVILSGARRVGKTTIMYQLIQSLLQRGVPAKSIVYLSFDNPIIKLGGFES
                     VLRAYRNAYPTEERCFFFFDEVQYAEDWEAWIKTLYDTQPELRLVATGSASPALEKGS
                     AESGVGRWSVLKIPTLSFYEYCDLLQLPERPRLEAGI"
BASE COUNT          345 a          484 c          506 g          356 t
ORIGIN      
        1 cgccgatcac gggcgcgcgg agggcgtggg cgaagcgcga ggagtggcgc gagcgtttca
       61 gcgggtcggc gtcgagcagg atgacgcccg ccttcaccga cgtcatgata agcgcgtggt
      121 agaagtgcgg gatgtcgaag aactctcccg gcgtgtccac cgcatcgtcc ccgaagctca
      181 cagcctcggt cttgcggacg tcctgctgcc agatgccgag tgccttcagc aggctcgtct
      241 tgccggagcc ggtcggcccc acgatcatga cgcgtttcat ggagtggcca ggggcatgcc
      301 gcatatcccc atcatctacg cttcctggga cgcgagcgat cgacagtgag tctcacgaaa
      361 aggccgttca tcggtcgcct gcttacgtgc gggtcagcgg cgcgggggtg aagccgagca
      421 cggtggcgag cgtccggacg gttccctcca gcgagctttc cacggcggcg acgtcgccgg
      481 tgaagaggac gcagccggtg aagcggtcga tgaagtccat cgcgatgtcg cccgacttgg
      541 aggcaatgtc agcggcgatg atggctccct cgccgggcgt gatggtcatg atgccgatgg
      601 cgcccgactt ctccacgccg atcagcgagc agagctcggg acgcgggttg cagatcacat
      661 gcgccagcgt cacctgtttt ccaggaacgt actcctgcac caccctcgcc ttggtttcct
      721 ccgtcatgaa gccgccccct tccgattttt cacgcatctt cgtgagaatg ataccataca
      781 ttcactccgt gttgtactgc cttatcgggc ctcgttgtaa aaagagcgga gccgtttcaa
      841 aaagacggat ttctctcctg agaacgcttc tgaagcggag cacaaagcgt tcgcggcagt
      901 ttgactctac gctccgttcc ccaaaagatg ggcgtagcgg cggcgtccgt gaatcactct
      961 gtgaacctcg acggtttctt ctatcgtaga gaaaacagaa agtgcaccct cccgcatcca
     1021 ctttaaaaaa cagtacgatg tgcattatta ttgactaaat ggagagtacg atataccatt
     1081 tttctggagg tggtcctcaa tgcttgcgtt cacggatgaa aagctgttga atcttttaat
     1141 acagtacaat ccctggtgga gaggagttcc catttcagag aagacatctc cgcccaaacg
     1201 gcggattgcc tactacgaag cggagaagat gctgcttcac ccctctctgc gccggttcgt
     1261 gatactctcc ggcgccagac gcgtgggcaa gaccacgatc atgtaccagc tgatacaaag
     1321 tctcttacag agaggcgtgc ccgcgaaaag catcgtgtat ctgtcgttcg acaatcccat
     1381 cattaaactt ggcggtttcg agagcgtgtt gcgggcgtat cggaacgcct atccgaccga
     1441 ggaaaggtgt ttcttcttct ttgacgaggt gcagtacgcg gaggactggg aggcgtggat
     1501 caaaacgctg tatgacacgc aaccggagct gcggctggtc gccacggggt cggcgagccc
     1561 cgctctggaa aaaggatcgg cggagagcgg cgtaggacgt tggagcgtct tgaagatccc
     1621 gacgctttcc ttttacgagt actgcgatct gctgcaactc cccgagcggc cccgccttga
     1681 agcgggtatt c
//
LOCUS       DAOTIL010000676         1686 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminobacteriaceae bacterium isolate AMR_MDS_3026
            NODE_85113_length_1686_cov_2.517474, whole genome shotgun sequence.
ACCESSION   DAOTIL010000676 DAOTIL010000000
VERSION     DAOTIL010000676.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868050
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminobacteriaceae bacterium (wastewater metagenome)
  ORGANISM  Aminobacteriaceae bacterium
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae.
REFERENCE   1  (bases 1 to 1686)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1686)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:19:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,224
            CDSs (total)                      :: 3,198
            Genes (coding)                    :: 3,184
            CDSs (with protein)               :: 3,184
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1686
                     /organism="Aminobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_85113_length_1686_cov_2.517474"
                     /isolate="AMR_MDS_3026"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:3034609"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(118..930)
                     /locus_tag="P5201_12070"
     CDS             complement(118..930)
                     /locus_tag="P5201_12070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013048061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter transmembrane
                     component T"
                     /protein_id="HRV99326.1"
                     /translation="MKFLNHLTFGQYVPAESLVHRLDPRCKILAVLVVLTGIFLARHI
                     LVFAAWGGLLFSLAVLSHLPFRLVLRSAKPVLILVFFTAFIHVFFTDGTPLLEIGFIT
                     ITREGVSGAVYMGLRLLFLVLFASFLTLTTKPMELSDGLERLFSPFVRFGFPAHELAM
                     MMTIALRFIPTLLDETDRIMKAQLARGAAFDRGNLWKRLKAFLPVLIPLFVIVFQRAE
                     DLATAMEARCYRGGEGRTRMHPLVWRTGDTLGALCVLAIVTGLIAGDRSFLS"
     gene            complement(927..>1686)
                     /locus_tag="P5201_12075"
     CDS             complement(927..>1686)
                     /locus_tag="P5201_12075"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HRV99327.1"
                     /translation="VGHTGSGKSTLAQHLNGILFPERGTVAVDGMEVKEKSARLRDVR
                     RAVGLVFQYPEQQLFAENVLEEISFAPRNWGIPESRIPALASSALSLTGLDETYLPRS
                     PFHLSGGEKRKVAIASVLAGEPSYLVLDEPTAGLDSWSRRELVSLLVRLKGEGLGIIL
                     VTHDLDIALQHSDSILMLDRGRCIIMDTPRKVMEHLQEHPAPGLHLPEIAALSASLRN
                     ADPGAPLTWDWKELKRWVVTRAVAAHPHLPGGDS"
BASE COUNT          369 a          481 c          560 g          276 t
ORIGIN      
        1 cccgcccgga gatgatccgg aggctgtcct cgatcttctc ctgaacgccg agaccgctgg
       61 tctggcgctg ccacccggag aaggatgatc cccggtatcc gacgaccgcg gcgtacttca
      121 cgaaagaaag ctccggtctc cggcgatcag tcccgtgaca atcgccagga cgcacagcgc
      181 gccgagcgta tctccggtcc tccagacaag cggatgcatc ctggtgcgcc cctccccccc
      241 tctgtaacac cgcgcctcca tggcggtggc aagatcctcc gccctctgga agacgatgac
      301 gaagagaggg atgagtacgg ggaggaacgc cttgaggcgt ttccagagat tcccgcggtc
      361 gaaggccgct cctctcgcga gctgcgcctt catgatgcgg tcggtttcgt cgagaagggt
      421 ggggatgaag cggagcgcga tcgtcatcat catggccagt tcatgcgcag ggaagccgaa
      481 ccggacgaac ggggagaaga gacgttcaag cccgtcggag agctccatgg gcttggtcgt
      541 gagcgttaaa aacgaggcga aaagaacgag gaagagaagc cgcagcccca tgtagacggc
      601 gccggagact ccctcgcggg taatggttat aaagccgatt tccagcagcg gcgttccgtc
      661 ggtgaaaaaa acatgaatga acgccgtaaa gaagacgagt atgagcacgg gcttcgccga
      721 gcgaaggaca aggcggaacg gaaggtgcga gagcacggca agagaaaaga gaagccctcc
      781 ccatgccgca aaaacaagaa tgtgccgcgc aaggaagatc cctgtgagga ccaccaacac
      841 ggcgagaatc ttgcagcgcg ggtcgaggcg gtgcacaaga gattcggcgg gcacgtactg
      901 accgaacgtg agatggttca ggaatttcat gagtcgcctc cgggaaggtg agggtgtgcg
      961 gcaacagccc gcgttactac ccagcgtttc agctctttcc agtcccacgt tagcggcgcg
     1021 cccgggtccg cgtttcgcag cgaggcggag agcgccgcta tctccggcag atggagaccg
     1081 ggcgcggggt gttcctgaag atgttccatg acctttcggg gcgtgtccat gatgatgcag
     1141 cgcccgcggt cgagcatcag gatcgagtcg ctgtgctgaa gggcgatatc gagatcgtgc
     1201 gtcaccagaa tgatgccgag tccctctccc ttcagacgta caaggaggga gacaagttcc
     1261 cgtctcgacc aggagtcgag ccccgccgtc ggttcgtcga ggacgaggta ggagggctcg
     1321 cccgcaagaa cggaggcgat ggcgaccttt cgcttctcgc cgcccgagag gtggaagggg
     1381 ctgcgcggga gataagtttc gtcaaggccg gtcagagaaa gagcagacga ggcaagagca
     1441 ggtatcctgc tttcgggaat gccccagttg cgaggggcga aggagatctc ctcaagaacg
     1501 ttctcggcaa agagctgctg ttcggggtac tgaaaaacca ggccgaccgc gcggcggacg
     1561 tcccgaagcc gggcgctctt ctccttgacc tccatgccgt cgacggcaac ggttcccctt
     1621 tcggggaaga gaatgccgtt caggtgctgc gcaagagtcg acttgccgct gccggtgtgg
     1681 ccgaca
//
LOCUS       DAOTIL010000677         1683 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminobacteriaceae bacterium isolate AMR_MDS_3026
            NODE_85303_length_1683_cov_3.947174, whole genome shotgun sequence.
ACCESSION   DAOTIL010000677 DAOTIL010000000
VERSION     DAOTIL010000677.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868050
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminobacteriaceae bacterium (wastewater metagenome)
  ORGANISM  Aminobacteriaceae bacterium
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae.
REFERENCE   1  (bases 1 to 1683)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1683)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:19:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,224
            CDSs (total)                      :: 3,198
            Genes (coding)                    :: 3,184
            CDSs (with protein)               :: 3,184
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1683
                     /organism="Aminobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_85303_length_1683_cov_3.947174"
                     /isolate="AMR_MDS_3026"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:3034609"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..524)
                     /locus_tag="P5201_12080"
     CDS             complement(<1..524)
                     /locus_tag="P5201_12080"
                     /inference="COORDINATES: protein motif:HMM:NF019310.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HRV99328.1"
                     /translation="MSTSRSTYRSLFADPSFPLFIILTSSFFLTVFFRISASVILPLE
                     GERLGMSASLVGFISSVHFYAYALMQPVSGILHDRYGPVRVVTCGLLLTAASCLLLTI
                     VRTPFTLGVWRLVSGFGVSPMYSATLVFLAFAFPAERYCFYAGINFAVSSLGAIVSVA
                     PLGFALDTFGIPAT"
     gene            complement(695..1171)
                     /gene="fliS"
                     /locus_tag="P5201_12085"
     CDS             complement(695..1171)
                     /gene="fliS"
                     /locus_tag="P5201_12085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012869575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009288 - bacterial-type flagellum
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar export chaperone FliS"
                     /protein_id="HRV99329.1"
                     /translation="MFWPKNRSRAQETYTENAIRTASREQLLLITYDIGIQSCLAAES
                     ALSNHLYDETNEHLKRAQNVVRELMITLRVNKDNPVTTNLMRLYDFMYTTLIKANTAK
                     DPEKIAEVRAMLEDLKATWEESIVKIAEEYDESKKSEYLNNLPSVQRNGDRFSARG"
     gene            complement(1355..>1683)
                     /locus_tag="P5201_12090"
     CDS             complement(1355..>1683)
                     /locus_tag="P5201_12090"
                     /inference="COORDINATES: protein motif:HMM:NF012904.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="dihydrodipicolinate synthase family protein"
                     /protein_id="HRV99330.1"
                     /translation="GGKDTLIYGALAHGADGAVATMANFCPELVVSIYEKYVAGDLKG
                     SLEAQFRLNPVRLAMDRASFPVATKDMAKLLGLDVGEPFRPNLSSAGDVLEFMKKRMQ
                     QGGLLP"
BASE COUNT          355 a          494 c          499 g          335 t
ORIGIN      
        1 aaggtcgcgg ggatgccgaa cgtatcaagc gcgaatccga ggggtgcgac cgagacgatg
       61 gcccccaggc tggagacggc gaagttgatc cccgcgtaga agcagtaccg ctccgcaggg
      121 aaggcgaagg ccaaaaagac aagcgtcgcg gagtacatgg gggagacgcc gaacccggag
      181 accagccgcc agacgccgag cgtgaagggc gtccggacga tcgtcagcag caggcacgaa
      241 gccgcggtga gcagcagacc gcaggtcacc acccgcaccg ggccgtacct gtcgtgcaga
      301 atgccggaga caggctgcat cagcgcgtac gcgtaaaaat ggacgctgga gatgaacccc
      361 accagagacg cgctcatgcc gagccgctct ccttccagcg gcagaataac ggaggccgaa
      421 atcctgaaga agaccgtcag gaaaaacgat gaggtcagta tgatgaacag gggaaaggac
      481 gggtcggcaa aaagcgacct gtaagtcgaa cgagaagtgc tcacagcaaa gctcctttgt
      541 cgcagtcaat gttttgaacg gggtacagca taccatagaa aatgaactgc gggaagtcgg
      601 gaaacatact tataatgaag agaaataacc agcttcttcc gtgttgctgc tattcttttc
      661 cggagcgtcc gcctcgctgg aacttcgccc ccctctaccc ccgggccgaa aacctgtcgc
      721 cgtttcgttg caccgagggc aggttgttca gatactcgct cttcttgctt tcgtcgtact
      781 cctccgctat cttgacgatg ctctcttccc aggtcgcctt caggtcttcg agcattgcgc
      841 gcacctccgc gatcttctcc ggatccttcg cggtgttcgc cttgatgagc gttgtgtaca
      901 tgaagtcgta gaggcgcatg aggttcgtcg tgaccggatt gtccttattc acgcgcagcg
      961 tgatcatcag ctccctcacg acgttctgcg cccgcttgag gtgttcgttc gtctcgtcgt
     1021 agagatggtt cgacagcgcg gactccgcgg ccaggcagct ctgaatgcct atgtcgtagg
     1081 taatcagcag gagctgctcg cgcgacgcgg tgcgaatggc attttcagta taggtttcct
     1141 gcgcccggct cctgtttttt ggccagaaca tgacaactcg ccccctcccg cggagatttt
     1201 cgcaaaacgt tcaaagagtt tcctcgatgt cttttatttt cggtagagcg gaggggcagg
     1261 ttaagtctga acagacagca acaggaagag agttcacaga gggcgtgccc gcaattttcc
     1321 gcggatacgc cctctgcatt ttttcgggga ggcgctacgg cagcagtccg ccctgctgca
     1381 ttctcttttt cataaactcc agcacgtcgc ccgcgctgct caggttcggg cggaagggct
     1441 cgccgacatc cagccccagt aatttcgcca tgtccttcgt ggcgacgggg aagctcgccc
     1501 ggtccatggc gaggcgcacc ggattcagcc ggaactgcgc ctcaagagag cccttcaggt
     1561 cgcccgcgac gtacttctcg tagattgaga ccacaagctc gggacagaag ttcgccatgg
     1621 tggcgaccgc gccgtccgcg ccgtgggcga gcgcgccgta gatcagcgtg tcctttcccc
     1681 cga
//
LOCUS       DAOTIL010000678         1682 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminobacteriaceae bacterium isolate AMR_MDS_3026
            NODE_85393_length_1682_cov_3.079902, whole genome shotgun sequence.
ACCESSION   DAOTIL010000678 DAOTIL010000000
VERSION     DAOTIL010000678.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868050
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminobacteriaceae bacterium (wastewater metagenome)
  ORGANISM  Aminobacteriaceae bacterium
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae.
REFERENCE   1  (bases 1 to 1682)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1682)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:19:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,224
            CDSs (total)                      :: 3,198
            Genes (coding)                    :: 3,184
            CDSs (with protein)               :: 3,184
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1682
                     /organism="Aminobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_85393_length_1682_cov_3.079902"
                     /isolate="AMR_MDS_3026"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:3034609"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..521)
                     /locus_tag="P5201_12095"
     CDS             complement(<1..521)
                     /locus_tag="P5201_12095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006301239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009428 - bacterial-type flagellum basal
                     body, distal rod, P ring [Evidence IEA];
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body P-ring protein FlgI"
                     /protein_id="HRV99331.1"
                     /translation="MKRYGVLPLALLLLFITACGAAASHPDVRIKDLADVEGVRSNQL
                     VGLGLVMGLQGTGDRSAMATQMLRNIMSQFGISVNEKAVRSRNVAVVTLTAELPAFAR
                     PGQTIDVTASAMGDAKSLQGGTLLQAPLRAADGNVYAVAQGPLMVGGFAVEGAAGAVT
                     KNIVTVGRIPGGA"
     gene            complement(535..1113)
                     /locus_tag="P5201_12100"
     CDS             complement(535..1113)
                     /locus_tag="P5201_12100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005661765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009427 - bacterial-type flagellum basal
                     body, distal rod, L ring [Evidence IEA];
                     GO_function: GO:0003774 - cytoskeletal motor activity
                     [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body L-ring protein FlgH"
                     /protein_id="HRV99332.1"
                     /translation="MKRSACVLILLLCASVLTGCAAEAASLWNDNTNWIADRRPSQVG
                     DIVTVFVDEKTKTKDEGTTEASKTNDNNVDDGFGIFDFIRAFGFSSSSKMTGDGSTER
                     THSAKGQITCLVTDVLPNGNLVIEGTRDIATHEETLQLHVIGVIRPQDVDSYNKIRSD
                     LVANAEIDIKGKGTISRLQKPGILTQILQAIF"
     gene            complement(1117..>1682)
                     /gene="flgA"
                     /locus_tag="P5201_12105"
     CDS             complement(1117..>1682)
                     /gene="flgA"
                     /locus_tag="P5201_12105"
                     /inference="COORDINATES: protein motif:HMM:TIGR03170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009428 - bacterial-type flagellum basal
                     body, distal rod, P ring [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="flagellar basal body P-ring formation chaperone
                     FlgA"
                     /protein_id="HRV99333.1"
                     /translation="SLVAIVKRLSGWRWTIDVEPLGTVPPGRLVSPLSIPPGTGSATL
                     RFIDDRGNERALAVRLSWSQPAVVTTRPLERGAVIAAADVTVRDVRVMRSQPLASSPN
                     EVIGRSLGRNLTAGEPVLLNSLSRTPIIQRGDSVIITVRKPGFLVEVRGEALDAGAEG
                     ESIRVRNLQSRAVIQAVVVGSGRVEVR"
BASE COUNT          293 a          524 c          518 g          347 t
ORIGIN      
        1 atggcgcctc ctggaattct gccgacggtg acgatgttct tggtgactgc tcccgccgct
       61 ccctcgacgg cgaacccgcc caccatcaat ggcccctggg ccactgcgta gacgttgccg
      121 tccgccgccc tgagcggcgc ctgaaggagc gtgcccccct gaagactctt cgcgtccccc
      181 atcgcgctgg ctgttacgtc gatcgtctgt cccggacggg cgaacgcggg aagctcggcg
      241 gtcagggtga cgaccgccac gtttctgctg cgcacggcct tttcgttcac cgagataccg
      301 aactggctca tgatgttgcg gagcatctgc gtggccatcg cgctgcggtc gcccgtcccc
      361 tggaggccca tgacaaggcc gagaccgacg agctgatttg aacggacccc ctcgacatcg
      421 gcgaggtcct tgatgcggac gtcggggtga gaggcggcgg cgccgcaggc ggtgatgaag
      481 agcaaaagaa gtgcaagcgg aagaactccg taccgtttca tggtccgcgc ctccttagaa
      541 gatcgcctga aggatctgcg tcaggatgcc cggcttttgc agacgggaga tggtaccctt
      601 ccccttgatg tcgatctcgg cgttcgcgac gaggtcgctc ctgatcttgt tgtagctgtc
      661 cacgtcctgg ggccggataa cgccgatgac atggagctga agcgtctcct cgtgggtggc
      721 gatgtctctc gttccctcga taaccaggtt accgttgggg aggacgtcgg tgacaaggca
      781 ggtgatctgc cccttggcgc tgtgggtccg ctcggtggaa ccgtctccgg tcattttgct
      841 cgacgagctg aagccgaacg cgcggatgaa gtcgaagatg ccgaaaccgt cgtcgacgtt
      901 gttgtcgttc gtcttgctcg cctcggtcgt cccttcgtcc ttggtcttgg tcttttcgtc
      961 gacgaagaca gtcactatat cgcccacctg cgacggtcgg cggtcggcta tccagttcgt
     1021 attgtcgttc cacagcgacg cagcctccgc cgcgcagccc gtgaggaccg acgcacacag
     1081 cagaaggatg agaacgcacg cgcttcgttt cattacttac cgcacctcca ctcttccgga
     1141 tccgacgacg actgcctgga tcacggctct gctttgcagg ttgcgcaccc ggatactctc
     1201 tccttcggcg cccgcatcaa gggcttctcc tctgacctcg acgagaaacc ccggctttct
     1261 tacggttatt ataacagagt ctcccctttg aattatcgga gtacgagaga gggagttgag
     1321 aagtacgggt tcgcccgccg taaggtttct tccgaggctg cgcccgatca cttcgttcgg
     1381 tgacgaggcc agcggctgtg aacgcatgac gcgcacgtct cgaaccgtga cgtcggccgc
     1441 tgcgatgacc gctccccttt cgagggggcg cgttgtgacg acggccggct gactccagga
     1501 gagtcgaacg gcaagcgccc gttcattccc ccggtcgtcg ataaaacgaa gggttgcaga
     1561 acccgttccc ggcggtatcg aaagcggaga gacaaggcgg ccgggaggaa ccgttccgag
     1621 gggttcgacg tcaatcgtcc atcgccaccc ggaaaggcgc ttgacgatgg caaccagaga
     1681 tt
//
LOCUS       DAOTIL010000679         1682 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminobacteriaceae bacterium isolate AMR_MDS_3026
            NODE_85429_length_1682_cov_2.161647, whole genome shotgun sequence.
ACCESSION   DAOTIL010000679 DAOTIL010000000
VERSION     DAOTIL010000679.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868050
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminobacteriaceae bacterium (wastewater metagenome)
  ORGANISM  Aminobacteriaceae bacterium
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae.
REFERENCE   1  (bases 1 to 1682)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1682)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:19:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,224
            CDSs (total)                      :: 3,198
            Genes (coding)                    :: 3,184
            CDSs (with protein)               :: 3,184
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1682
                     /organism="Aminobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_85429_length_1682_cov_2.161647"
                     /isolate="AMR_MDS_3026"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:3034609"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..224
                     /locus_tag="P5201_12110"
     CDS             <1..224
                     /locus_tag="P5201_12110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006584278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRV99334.1"
                     /translation="APSVPKVGVLLVEEGEPGGPFGGKSIGEICTVPVAPAIANAVNA
                     SLGTSMTELPFTPERILAALSALRETNAR"
     gene            283..>1682
                     /locus_tag="P5201_12115"
     CDS             283..>1682
                     /locus_tag="P5201_12115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008712204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic amino acid ammonia-lyase"
                     /protein_id="HRV99335.1"
                     /translation="MSIVLTGNTLTLDQLHAIARMNEPVEIHPDAARRLEESRKLVFD
                     LVESNVPVYGFNTGVGWNKDKHIAADFFKEYNENVIHSHSLGVAPEASREEVRAAMAI
                     RLNCLLRGNTGIQPAIARRYAEFLNLGITPVVPERGSVGESDIAVLSHIGLAMIGEGD
                     ADYDGTRMTSARAHAETGLQPVELGPKDALAIVNSSAFGMAQGAMVLCDLLDLVDQAD
                     LIYAVSLEGLNGNTSPLEPKVHEARRLQGQAKSAALVNRFLEGSFIFSPDPEKPVQDP
                     LCFRGGAYVHGSLRDALEYVVEKSLVQMNCSDDNPCLLLDERRIVSCSNFETTTLAAG
                     FEMLSVVLSHLSRMSCYRMIKLGNPTFTGLTRFLTPAEGKVHAFGTIQKTFSLLDTEI
                     RHLSNPSTVDYLSLAGDIEDHANNLPHIAQRVRTIADNLKYILGMEMMHAAQAVSLRK
                     HAAPGLPLGKGTAAAY"
BASE COUNT          354 a          536 c          500 g          292 t
ORIGIN      
        1 acgccccttc ggtgccgaaa gtcggcgtgt tgcttgtcga agagggcgaa ccgggaggcc
       61 cgttcggagg gaagagcatc ggcgagattt gcacggtgcc ggtagccccg gccatcgcaa
      121 acgccgtcaa cgcatcgctc gggacgtcga tgacggagct gccgttcacg ccggaacgca
      181 ttctggccgc tctgtcggca ctgcgggaaa cgaacgcacg ataagaccgc ggcgagtaaa
      241 aagctcagcg gagcgaacct ggacaggatg gaggaatgtc atttgagcat cgtactgacc
      301 ggaaacaccc tcacgttgga tcagctgcac gcaatcgcca gaatgaacga gcccgtggag
      361 attcaccccg acgccgcgcg tcgcctggag gagtcccgaa agcttgtttt cgacctcgtc
      421 gagagcaacg taccggtgta cggattcaat accggcgtgg ggtggaacaa ggataaacat
      481 atcgcggcgg acttctttaa ggaatacaac gaaaacgtca tccactcgca ctccctcggc
      541 gtcgctcccg aggcttcgcg cgaggaggtt cgggcagcga tggcaatccg cctgaactgc
      601 ctccttcgcg gcaacacggg catacagccc gcaatagcgc ggcggtatgc ggagttttta
      661 aacctcggca ttaccccggt agttcccgaa cgcggttccg tcggagagtc cgacatcgcc
      721 gtcctctcgc acatcggact cgcgatgatc ggcgaaggcg acgcggacta cgacggaacg
      781 agaatgacct cggcccgcgc ccacgctgaa acggggctgc aaccggtcga gctcggcccg
      841 aaggacgcgc ttgcgatcgt caattcaagc gccttcggca tggcgcaggg ggcgatggtc
      901 ctctgcgatc tgcttgatct cgtcgaccag gccgacttga tctacgcggt ctctctcgaa
      961 gggctgaacg ggaacacctc cccgctcgaa ccgaaggtcc acgaagcgcg ccgtcttcaa
     1021 gggcaagcga agagcgccgc gctcgtcaac cggtttctgg aagggagctt catcttctct
     1081 cccgatccgg aaaaacccgt acaggacccg ctctgcttcc gcggaggagc ctatgtccac
     1141 ggttcgctcc gcgatgcgct ggagtatgtc gtcgagaaga gcctcgtcca gatgaactgc
     1201 tccgacgaca acccctgcct tctgctggac gagcggcgca tcgtctcgtg ctcgaacttc
     1261 gagacgacca ccctggccgc cggattcgaa atgctttccg tcgtgctgag ccacctttcc
     1321 cggatgtcct gctacaggat gatcaagctc ggcaacccga cgttcaccgg actgacgcgc
     1381 tttctgacgc ccgcggaagg gaaggtgcac gccttcggaa cgatccagaa gacgttctcc
     1441 cttctggaca ccgagatacg ccatctctcc aacccctcca cagtggacta cctgtccctc
     1501 gcaggagaca tcgaggatca tgcgaacaac ttgccccata tcgcacagag ggtgcgcacg
     1561 atcgcggaca acctgaagta catcctgggc atggagatga tgcacgccgc gcaggcggtc
     1621 tcgctccgaa aacatgccgc cccgggtctt ccgctgggaa aaggaacggc cgccgcatac
     1681 ga
//
LOCUS       DAOTIL010000680         1681 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminobacteriaceae bacterium isolate AMR_MDS_3026
            NODE_85474_length_1681_cov_2.929274, whole genome shotgun sequence.
ACCESSION   DAOTIL010000680 DAOTIL010000000
VERSION     DAOTIL010000680.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868050
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminobacteriaceae bacterium (wastewater metagenome)
  ORGANISM  Aminobacteriaceae bacterium
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae.
REFERENCE   1  (bases 1 to 1681)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1681)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:19:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,224
            CDSs (total)                      :: 3,198
            Genes (coding)                    :: 3,184
            CDSs (with protein)               :: 3,184
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1681
                     /organism="Aminobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_85474_length_1681_cov_2.929274"
                     /isolate="AMR_MDS_3026"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:3034609"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..202)
                     /locus_tag="P5201_12120"
     CDS             complement(<1..202)
                     /locus_tag="P5201_12120"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRV99336.1"
                     /translation="MERIGDSLSRGMDICSSKDGGAPMGTPHVADAVTTLDRRRVSGV
                     SSGLFRCPSLLRRCSTGWMPSSS"
     gene            331..606
                     /locus_tag="P5201_12125"
     CDS             331..606
                     /locus_tag="P5201_12125"
                     /inference="COORDINATES: protein motif:HMM:TIGR03793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NHLP leader peptide family RiPP precursor"
                     /protein_id="HRV99337.1"
                     /translation="MDGKEMRKLLRKLLAKAWADAKFKAKLIADPASVLRTEGMEVPE
                     GMKIHVVENTDDRLYLVIPRSPEELADEELDFLSAEGLSARRTAPDR"
     gene            complement(638..>1681)
                     /locus_tag="P5201_12130"
     CDS             complement(638..>1681)
                     /locus_tag="P5201_12130"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="SYNERG-CTERM sorting domain-containing protein"
                     /protein_id="HRV99338.1"
                     /translation="GNLTMNSCTLTNNTNSLAFGSAVTNAYLGGGLHASAALNGCTIE
                     NNDVAGSLGGAIYNGDILVMSNTKVCNNTTGKGSGLQSRKGGGLYVGSRSNTTLTNGC
                     RVTGNDPSQLYKEETYFSTPVFTYDSTCVIGDAPNKSATAFSGYTGETAPEPRSIKDD
                     ADVAEVKRKLADSGSDLFATVSRALSSDLGRTPDGTSATLAGLTASLYYANTFENVGV
                     ESQDLVVEYTASWPENARYYALFARADNAGYELAERGIQFELKAGQSLPDGATPPDFY
                     VPGEGLMTWRNVVTDGGPYDLNPAAGVVTFRVCSVRAAEAQTASSGGSGCSALGASPF
                     VLLLGLPLLLRKL"
BASE COUNT          312 a          505 c          545 g          319 t
ORIGIN      
        1 gagacgacga cggcatccac cccgtcgagc acctcctcaa gagcgatggg cagcggaaaa
       61 ggcccgatga tacgccggaa acgcgccgac ggtcaagcgt cgttaccgcg tcggcgacat
      121 ggggcgtgcc catgggggcg ccgccgtctt ttgaagagca gatatccatg ccgcgggaga
      181 gggagtctcc gattctttcc attcgggctg aatcacgaag cgctcttcca tcggcacggc
      241 gaggtagtcg tttccgggtg agaaaaccta ctcctcacgc agaaaaagcg tataattccg
      301 aataggatat gtattcggga gggattggct atggacggga aagagatgcg taagcttctg
      361 aggaaactgc tcgccaaggc gtgggcggac gcgaagttca aagcgaagct gatcgccgat
      421 cccgcgtcgg ttttgagaac cgaggggatg gaggttccgg agggcatgaa aatccatgtg
      481 gtcgagaaca cggacgaccg gttgtatctg gtcatcccga gaagccccga ggaactggcc
      541 gacgaggaac tcgacttctt gtctgcggaa ggcctatcgg cccgtcgaac ggcgccggac
      601 cgataggcga ataattacgg cgtcgtttag ggatgtctta cagttttcgc agcagtagcg
      661 gcaatcccag aaggagcacg aaaggcgacg cgcctaaggc cgagcagccc gaaccgccgg
      721 aggaggccgt ctgcgcctcg gcggcgcgca cggagcacac gcggaaggtg acgacgccgg
      781 ccgccgggtt caggtcgtac ggtccgccgt ccgtcacgac gttccgccac gtcatgagcc
      841 cctcgcccgg gacatagaag tccgggggcg tcgcgccgtc gggcaggctc tgcccggcct
      901 tcagctcgaa ctggatgccg cgttccgcaa gttcgtaccc cgcattgtcg gcgcgggcga
      961 agagcgcgta gtaccgcgcg ttctcgggcc acgacgccgt gtactcgaca acgagatcct
     1021 gactctcgac gcccacgttc tcgaacgtgt tcgcgtagta gagggacgcg gtcagacccg
     1081 cgagcgtcgc gctcgttccg tcgggagttc tgccgaggtc cgaagagagg gcccggctga
     1141 cggtcgcgaa gagatcgctt ccggaatcgg ccagtttgcg cttcacttcg gcgacatcgg
     1201 cgtcgtcctt gatcgagcgc ggttccggcg ccgtttctcc cgtgtatccc gagaacgccg
     1261 tcgccgactt gttcggcgcg tcgccgatca cgcacgtgct gtcgtacgtg aagaccggcg
     1321 tcgagaagta cgtctcctcc ttatagagct gcgacggatc gtttccggtc actcggcatc
     1381 cgttcgtgag cgttgtgttc gagcgagaac ccacgtagag tcctcctccc ttgcggctct
     1441 gcagcccgct gccctttccg gtcgtgttgt tgcaaacttt ggtgttgctc atgacgagga
     1501 tgtccccgtt gtagatcgcg ccgccgaggc tccccgcgac gtcgttgttt tcgatcgtgc
     1561 agccgttcag cgcggcgctc gcatggagac cgccgccaag gtacgcgttc gtgacggcgg
     1621 aaccgaacgc caaagagttc gtgttgttcg tcagcgtgca cgagttcatc gtcaaatttc
     1681 c
//
LOCUS       DAOTIL010000681         1678 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminobacteriaceae bacterium isolate AMR_MDS_3026
            NODE_85675_length_1678_cov_4.842884, whole genome shotgun sequence.
ACCESSION   DAOTIL010000681 DAOTIL010000000
VERSION     DAOTIL010000681.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868050
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminobacteriaceae bacterium (wastewater metagenome)
  ORGANISM  Aminobacteriaceae bacterium
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae.
REFERENCE   1  (bases 1 to 1678)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1678)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:19:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,224
            CDSs (total)                      :: 3,198
            Genes (coding)                    :: 3,184
            CDSs (with protein)               :: 3,184
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1678
                     /organism="Aminobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_85675_length_1678_cov_4.842884"
                     /isolate="AMR_MDS_3026"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:3034609"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..498)
                     /locus_tag="P5201_12135"
     CDS             complement(<1..498)
                     /locus_tag="P5201_12135"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRV99339.1"
                     /translation="MKELEEDIHGGAAMRLKVFLAVFLLLFVLSGLSNAEIQSGIRLD
                     RETARVEVGKSVVVRALPTGSGDVWWNSSDTSVADFVTASDGLSVTVRGWRTGTAVIS
                     VHFSPYGSEVIHSASCQVTVAPNSNPYRVAAVRISPETLVVEKGRPVSLTATVVPETA
                     TNKNVT"
     gene            complement(636..987)
                     /gene="ssrA"
                     /locus_tag="P5201_12140"
     tmRNA           complement(636..987)
                     /gene="ssrA"
                     /locus_tag="P5201_12140"
                     /product="transfer-messenger RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00023"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00023"
     gene            complement(1008..1469)
                     /gene="smpB"
                     /locus_tag="P5201_12145"
     CDS             complement(1008..1469)
                     /gene="smpB"
                     /locus_tag="P5201_12145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013048730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SsrA-binding protein SmpB"
                     /protein_id="HRV99340.1"
                     /translation="MADRTVAQNRKARHDYFVLETFECGIVLTGTEIKSVRDGKINLK
                     EGYALIRGGELWLVGVHISPYEKGSYYNHEPLRDRKLLMKHHEILRLFSKVREKGLTL
                     VPLSVYLKEGKRAKVELALVKGKLLHDKRDSLAERDAKRDIERAVRRSDRD"
     gene            complement(1600..1677)
                     /locus_tag="P5201_12150"
     tRNA            complement(1600..1677)
                     /locus_tag="P5201_12150"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1640..1642),aa:Pro,seq:cgg)
BASE COUNT          394 a          478 c          384 g          422 t
ORIGIN      
        1 tgtcacattc ttgttcgttg ccgtctctgg tacaacagta gccgtcagag aaacgggtct
       61 ccctttttcg acaacgagag tctccggtga aatacggacg gctgccaccc tgtaaggatt
      121 tgaattcggg gccacggtca cctggcacga agcgctatga attacctccg agccgtaggg
      181 agaaaagtgt acggaaatca ccgccgtccc cgttctccac ccccttacag ttaccgacaa
      241 tccatcggaa gcggtaacga agtcagctac ggaagtatcg ctggaattcc accatacatc
      301 gccggatccg gtcggcaagg cacggacaac tacggatttc ccgacttcta ctcgcgctgt
      361 ttctctgtca aggcgaatgc cgctctgtat ttccgcattc gacaaaccgg aaagaacgaa
      421 caacagcaaa aagaccgcca ggaaaacttt caaccgcatc gcagcacctc cgtgaatatc
      481 ttcttcgagt tccttcatgt aatagtatca cgcttttccg cctcttgttg cgagaaaaat
      541 ctcgtgcttt tgcagacggt gtagaatggt ttgataaaaa aaccgccgct gccaggattt
      601 ttactgagca acgacggtga aatacgattt cctattggtg gaggcggggg gaatcgaacc
      661 cccgtccgac aagggccagc cgtggaagcg ctacgggcgt agttcccgat ttagtgtcgg
      721 ctcaaaactc ccgggaacag gattttctcg cccaagcccc cttatctttt ttccgggacc
      781 ggagacactc cccgaaaagc atccgccatt ttgtgtcgcc ttacgcagta acggcggctg
      841 ctgccgcgta agacgtgact gcattaagca gccagtgcgt agttattgtt gttgacgttt
      901 gtttttgtgt ccgcgtttaa cgaggttcgg acgacctcgg cccgctcttt cagaccctcc
      961 gtcttgccgt cgaaaccagt cgcccccatt tgtaaacgac tgtaacttca gtccctatcg
     1021 cttcgccgca cggcacgctc tatatctctt ttcgcgtccc gttcagcaag tgaatcccgt
     1081 ttgtcgtgaa gaagcttccc tttcaccagg gcaagctcga ctttcgccct cttaccctct
     1141 ttcaagtata ccgaaagagg aaccagagtc aatccttttt cgcgcacctt cgaaaaaagc
     1201 cgaagtatct catggtgctt catcagaagt tttctgtctc gcagcggctc gtgattgtaa
     1261 taacttcctt tttcatacgg ggaaatatga acgcctacaa gccataactc tcctccgcga
     1321 ataagggcat acccctcttt caggttgatc tttccgtcgc gcacagactt aatctccgtt
     1381 cccgtgagta caataccgca ctcaaacgtt tcaagaacga aatagtcgtg acgtgctttt
     1441 ctgttctgcg ctactgtgcg gtcggccatc tttttttcct cgttctacat tctagcatta
     1501 aggtcagtat tagtcaatac aaaaagcaaa atacagggct cttcttatac aaataaagag
     1561 gctccggaat tccggaacct ctcgggagaa tccaaaatat ggtcggggcg agaggatttg
     1621 aacctccgac ctcctggtcc cgaaccaggc gcgcttacca gactgcgcta cgccccga
//
LOCUS       DAOTIL010000682         1676 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminobacteriaceae bacterium isolate AMR_MDS_3026
            NODE_85806_length_1676_cov_4.417027, whole genome shotgun sequence.
ACCESSION   DAOTIL010000682 DAOTIL010000000
VERSION     DAOTIL010000682.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868050
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminobacteriaceae bacterium (wastewater metagenome)
  ORGANISM  Aminobacteriaceae bacterium
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae.
REFERENCE   1  (bases 1 to 1676)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1676)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:19:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,224
            CDSs (total)                      :: 3,198
            Genes (coding)                    :: 3,184
            CDSs (with protein)               :: 3,184
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1676
                     /organism="Aminobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_85806_length_1676_cov_4.417027"
                     /isolate="AMR_MDS_3026"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:3034609"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..455
                     /locus_tag="P5201_12155"
     CDS             <1..455
                     /locus_tag="P5201_12155"
                     /inference="COORDINATES: protein motif:HMM:NF019604.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HRV99341.1"
                     /translation="LVYSDGSTGDADFLVVATGARPSKVLTESGLEVDDEGAVIVDEY
                     LQSLSAKGVFGGGDCVAFQPMELERSASHALRQGRVLFANLLAYLSGEPMRPFIPKKE
                     PLTILNLGRTGLLVRKPFVARGRFALLLKRWIDRRIVKEFQSPSEEKS"
     gene            515..1405
                     /locus_tag="P5201_12160"
     CDS             515..1405
                     /locus_tag="P5201_12160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005660744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HRV99342.1"
                     /translation="MERLNIRKLLTQFGELVKAEWSTFWYATLGAVLMSFSIIALIVP
                     YRFAGAGLAGIALLARYVFDVSPAWVLAVGNLLLLGWGWRALSARFVLWTLYVSILSS
                     FAVAFFELFHYPVLANTLLAALLGGVCSGIGMGLVFRVGGSTGGTDVIVAVAKKRWGV
                     DMGMFSFYINTAILFLSWFAVDLEQLLMGGVLLYAESITIDSVLKSFDRRKQVTVITS
                     KMEEVKRFIVEDMEKGATVIRGEGAYTAAERPMIMVVLNRRQAMELKLFTVSVDPNAF
                     IIMADVAEVVGEGFKHWKNL"
BASE COUNT          320 a          453 c          526 g          377 t
ORIGIN      
        1 cgctcgtcta ctcggacggt tcgaccggag acgccgattt tctggtggtc gcaacgggcg
       61 cgcgtccgtc gaaagtgctg acggaatcag ggcttgaggt tgacgacgag ggcgccgtga
      121 ttgtcgacga gtacctccag tcgctttcgg cgaagggcgt gttcggcgga ggagactgcg
      181 ttgcctttca gcccatggag ctggagcgtt ccgcttccca tgccctccgg caggggcggg
      241 tgctcttcgc gaatcttttg gcgtacctct caggggagcc tatgcgtccg tttattccga
      301 agaaagagcc gctcacgatc ctgaatctgg gaagaacggg gctgcttgtc aggaagccgt
      361 tcgtcgccag aggtcgcttc gcgcttctgt tgaagcgatg gatcgaccgc cgtattgtga
      421 aggagtttca gagtccctcc gaggaaaaat cgtgagacga acgatgaaag ccttttgcgt
      481 accctcaatt tctctcagtt gaaaggaatg aatcatggag cgtttgaaca tacgaaagct
      541 cttgacgcag ttcggcgaac tggtcaaagc ggagtggagc accttctggt acgcaacgct
      601 tggcgcggtg ctgatgagtt tctccatcat cgccctcatc gttccgtacc gtttcgcggg
      661 cgccggcctc gcaggaatcg ctctgctcgc acggtacgtc ttcgatgtct cccccgcgtg
      721 ggtgctcgcc gtcggcaacc tgctgctgct gggctggggg tggagggcgc tctccgcgcg
      781 ctttgtcctc tggacgctct acgtctcgat actctcctcg ttcgccgtcg ccttttttga
      841 actcttccac taccccgtgc tggccaacac cctccttgct gcgctgctgg gcggggtgtg
      901 cagcggaatc ggcatggggc tggtcttccg cgtcggaggc tccaccggcg gaaccgacgt
      961 catcgtcgcc gttgcgaaga agcgctgggg cgtcgacatg ggaatgtttt ccttctatat
     1021 aaatactgcg attctcttcc tttcatggtt cgccgtggac ctcgaacagc tcctcatggg
     1081 aggagtgctg ctctacgcgg agagcatcac catcgacagc gtgctcaagt ccttcgaccg
     1141 gagaaagcag gttaccgtca tcacatcgaa gatggaggag gtcaaacgct ttatcgtcga
     1201 ggacatggag aaaggagcca cggttatcag gggcgaagga gcctacacgg ctgcggagcg
     1261 cccgatgatc atggtggtgc tcaaccggcg gcaggcgatg gagctgaaac tctttaccgt
     1321 ctccgtggac ccgaacgcgt tcatcatcat ggccgacgtc gccgaggtgg tgggcgaggg
     1381 gttcaagcac tggaagaacc tctagccttt ctgcgcggaa gaaccgccgg aagaatgctg
     1441 ccttacgaag ccgggaatct tcccggcttt tttttgtgtg ccccatttgt gaaattgata
     1501 actttattgt atgatagggg cgatgatcac ttagaggagg cgaccgtcat gaacggtgcg
     1561 ggagagaaga aggaaatagt cctctgcatg gggagttcgt gcttcgccag gggaaacgcg
     1621 aagacgcttg aagtagtgcg cagatacata gaggagcgga agctgcacgc ccggat
//
LOCUS       DAOTIL010000683         1672 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminobacteriaceae bacterium isolate AMR_MDS_3026
            NODE_86102_length_1672_cov_3.011750, whole genome shotgun sequence.
ACCESSION   DAOTIL010000683 DAOTIL010000000
VERSION     DAOTIL010000683.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868050
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminobacteriaceae bacterium (wastewater metagenome)
  ORGANISM  Aminobacteriaceae bacterium
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae.
REFERENCE   1  (bases 1 to 1672)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1672)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:19:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,224
            CDSs (total)                      :: 3,198
            Genes (coding)                    :: 3,184
            CDSs (with protein)               :: 3,184
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1672
                     /organism="Aminobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_86102_length_1672_cov_3.011750"
                     /isolate="AMR_MDS_3026"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:3034609"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..690
                     /locus_tag="P5201_12165"
     CDS             <1..690
                     /locus_tag="P5201_12165"
                     /EC_number="4.1.3.3"
                     /EC_number="4.2.1.41"
                     /EC_number="4.3.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009201998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009436 - glyoxylate catabolic process
                     [Evidence IEA];
                     GO_process: GO:0071704 - organic substance metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrodipicolinate synthase family protein"
                     /protein_id="HRV99343.1"
                     /translation="LPSDKKIIAGTGCESLKETVELTKQCAAAGADVALVVTPNYYKK
                     DMNEAALEKFYTMLADASPVPVMIYNMPGNSGVNVPSPLTLKLAAHPNITGIKDSGGN
                     IVQISEVLAKAPSDFSVFAGSGSYLLATLVLGGVGGTLAVANVVPDYCAEIQERFEKG
                     DLDGARKMQLALLPLNAAVTSRFGIGGMKAAMDMVGYKGGLPRLPIQPASEETKREIA
                     RILKELGVPAV"
     gene            693..>1672
                     /locus_tag="P5201_12170"
     CDS             693..>1672
                     /locus_tag="P5201_12170"
                     /EC_number="3.5.-.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR01879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016813 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds, in linear
                     amidines [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydantoinase/carbamoylase family amidase"
                     /protein_id="HRV99344.1"
                     /translation="MLVSGDRFLADLEALGRIGWVEGEGMRRPAYSASYDEARRFVAA
                     KMKEAGLTVRVDGVGNLFGRLEGSDPSLPSIMSGSHLDAVPGGGKYDGPLGVMAALEA
                     ARSIKERKLPLRHPFEAVGFTAEEGGEMGGTFGSRAMAGLLDEPLPEEKLASVGLTPE
                     MVRSSKRDPSRIACYIELHIEQGPFLERRGISIGIPTGIVGIGRYAVRLTGEANHAGT
                     TPMKERRDAMREAAELLSEWFAWTDARDDMVCNVGVFSIHPGAAAVVPDRAEFTLEIR
                     SLKDSVMEEAASAFRRLLEKRTRVAVSMEPLIWKGAVELSPLLQEAAEKA"
BASE COUNT          293 a          517 c          576 g          286 t
ORIGIN      
        1 ctcccctccg acaagaagat catcgccgga acggggtgcg agtcgctgaa ggagaccgtc
       61 gaactgacga aacagtgtgc ggccgcgggc gcggatgtgg cgctggtcgt caccccgaac
      121 tactataaaa aggatatgaa cgaggccgcg ctggagaagt tctacacgat gctcgccgac
      181 gcctccccgg tccccgtgat gatctacaac atgcccggca actccggcgt gaacgtcccc
      241 tctccgctga cgctcaagct tgccgctcat ccgaacatca ccggcatcaa ggacagcggc
      301 ggcaacatcg tgcagatctc cgaggtgctg gccaaggctc cctcggactt ttcggtcttt
      361 gccgggtcgg ggagctacct tctggccacc ctcgttctcg gaggcgtcgg cggcaccctt
      421 gccgtggcca acgtcgtccc cgactactgc gccgagattc aggaacgctt cgagaagggc
      481 gacctcgacg gagcgcggaa gatgcagctc gccctgctgc cgctgaacgc cgcggtcacc
      541 agccggttcg gcatcggcgg catgaaggcc gcgatggaca tggtcggcta caagggaggc
      601 ctcccgcgcc tgccgattca gcccgcgagt gaggagacga agagggagat cgcccggata
      661 ctgaaggagc tgggcgttcc ggcggtgtag ccatgctcgt ctccggtgat cgttttctcg
      721 ccgatcttga ggcgcttggg cgcattggct gggtcgaggg ggagggaatg cgccgtccgg
      781 catattccgc ttcttacgat gaggcgcgtc ggtttgttgc ggcgaagatg aaagaggctg
      841 ggttgaccgt cagagtggac ggcgtgggaa acctcttcgg caggctggag gggagcgacc
      901 cgtcgcttcc ctccatcatg tccggttccc atctcgacgc ggttcccgga ggcgggaagt
      961 acgacggccc tctcggcgtc atggccgcgc tggaggcggc gcgctccatc aaagaacgga
     1021 agcttccgct ccgtcacccc ttcgaggcag tgggcttcac cgccgaggag ggaggcgaga
     1081 tgggcggtac attcggaagc cgcgcgatgg cagggctgct cgacgagccg ctcccggagg
     1141 aaaaacttgc ctccgtaggg ctcaccccgg aaatggtgcg ctcgtcgaag agggatcctt
     1201 cgcggatcgc ctgctacatt gagctccaca tcgagcaggg gcccttcctt gagcggcggg
     1261 ggatatccat cggcatccct accggcatcg tcggcatcgg ccggtacgcc gtccgcctga
     1321 ccggcgaggc gaaccacgcc gggaccacgc cgatgaagga gcgcagggac gccatgcgcg
     1381 aggctgcgga gctgctgagc gaatggttcg cgtggacgga cgcgcgggac gacatggtct
     1441 gcaacgtcgg cgtcttctcg attcatcccg gggctgcggc ggtcgtgccg gaccgggcgg
     1501 agttcacgct tgagatccgc tcgctgaagg actcggtgat ggaggaggcc gcctctgcgt
     1561 tccgcaggct tctggagaag cggacccgcg tcgccgtgtc gatggaaccg cttatttgga
     1621 aaggagcggt ggaactctcc ccgctgctcc aggaggcggc cgaaaaggca tg
//
LOCUS       DAOTIL010000684         1671 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminobacteriaceae bacterium isolate AMR_MDS_3026
            NODE_86176_length_1671_cov_3.129950, whole genome shotgun sequence.
ACCESSION   DAOTIL010000684 DAOTIL010000000
VERSION     DAOTIL010000684.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868050
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminobacteriaceae bacterium (wastewater metagenome)
  ORGANISM  Aminobacteriaceae bacterium
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae.
REFERENCE   1  (bases 1 to 1671)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1671)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:19:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,224
            CDSs (total)                      :: 3,198
            Genes (coding)                    :: 3,184
            CDSs (with protein)               :: 3,184
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1671
                     /organism="Aminobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_86176_length_1671_cov_3.129950"
                     /isolate="AMR_MDS_3026"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:3034609"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..317)
                     /locus_tag="P5201_12175"
     CDS             complement(<1..317)
                     /locus_tag="P5201_12175"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRV99345.1"
                     /translation="MAKPIVARWEWRTFGAGFEEPEARIRQFEQGNYKESEEVYVLSR
                     NSDENVKVRDDLLDIKSLQEVNADGLEQWFPVMKAGFPIPIDDLRKLFSYFKAPEPIF
                     GRK"
     gene            complement(559..1605)
                     /locus_tag="P5201_12180"
     CDS             complement(559..1605)
                     /locus_tag="P5201_12180"
                     /inference="COORDINATES: protein motif:HMM:NF036995.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PDDEXK nuclease domain-containing protein"
                     /protein_id="HRV99346.1"
                     /translation="MTRFNDRHELLENLQRLLSESRRAALKAVNAEMLRAYWEMGKAL
                     SQWEETHGGSPDLLEGVAADLASDFGKAFSGKKLAAILKFFKEYENRPPLFRFREESD
                     LAESGLRSLDPGLSWSHYTVMMDIEDSAVRDEVERKVSREGWTASELEKQLWAAGEKT
                     SDVTDDMPLFEERTPPSPEDYVVDPHVQAFMGIPDRQRLTESILERALLDHLQRFLLE
                     SGKELYLVERQKKMELKGGARYIDLVFYSRSLHAFFLVELVCGDFSSEDEEKLSLVLE
                     YMSRESALSDENPPAALLIAVSRTAGRMKYLFASKERELEYRRLLPSEDQLAVEIQRE
                     QVDILKRYPHLEDE"
BASE COUNT          380 a          490 c          387 g          414 t
ORIGIN      
        1 tcttttctgc cgaaaatggg ttccggggct ttaaagtagg agaaaagctt ccggagatcg
       61 tctatcggaa tcggaaaccc ggccttcatg acggggaacc actgctccaa cccgtccgca
      121 ttcacttcct gaagcgattt gatatccaga agatcatccc gcaccttcac gttctcgtcg
      181 ctgttccggg agagtacgta gacctcctcg ctctctttat aattcccctg ttcaaactgc
      241 cggatccttg cctcgggctc ttcgaatccc gccccgaacg tcctccactc ccaacgcgcc
      301 actataggtt ttgccatgtt gtcccctcca tgttctgtat gaaatagtat agataaaaaa
      361 atcggaggag cattcgctcc cccgacaaaa aatatacaat aaccctactg gggcaaaaca
      421 ccctgaacgc atacgatgcg cagaatattc tgctttttca aggtccctgt gtattcacta
      481 tatcgcttct cggcatcttt tgtcaagcca ggattcgcac attttgtgta ttttcttcgc
      541 ctcagccccg aaaggacgct actcatcctc caggtgagga tatcgcttca gtatatcgac
      601 ctgttccctc tgaatttcga cggcaagctg atcctcagag gggagcaaac gtctgtactc
      661 cagctcccgc tctttcgacg cgaagagata cttcattctc ccggcggttc gagatacggc
      721 gataagcaac gccgcaggag gattttcgtc ggacagagcg ctttctcgcg acatatactc
      781 aaggacgagc gacagttttt cttcgtcttc actcgaaaaa tctccgcaga ctaactctac
      841 cagaaaaaac gcatgaagag aacgcgagta gaagacaagg tcgatatagc gcgcccctcc
      901 cttgagctcc atctttttct gacgttccac caggtagagc tctttgccgg attcaagaag
      961 aaaccgctga aggtgatcga gcagcgccct ttctagaatg ctctctgtca gccgctgacg
     1021 gtcgggtatt cccatgaacg cctgaacgtg gggatcgaca acgtagtctt ccggtgaagg
     1081 aggagtcctc tcctcgaaga gcggcatatc gtcggtcacg tcggaggttt tttctccggc
     1141 ggcccacagc tgcttttcca gttcggacgc cgtccatcct tcgcgcgata cctttcgttc
     1201 gacttcgtcc ctgacggccg aatcttcgat atccatcatc accgtgtagt ggctccagct
     1261 cagtccggga tcaagggagc gaaggccgga ttcggcgaga tcgctctcct ccctgaaacg
     1321 aaagagcgga ggacggtttt cgtattcctt gaaaaacttc aggatagcgg caagtttctt
     1381 tccggaaaac gctttgccga aatccgacgc aagatccgca gccactcctt caagaaggtc
     1441 gggagagcct ccgtgcgttt cctcccattg ggaaagcgcc ttccccatct cccagtacgc
     1501 acgaagcatc tcggcgttca ctgccttcaa agcagccctt cgagactctg aaagcagccg
     1561 ttgcagattt tccaacaact cgtgccggtc attgaaacgt gtcattctct cagcctcctt
     1621 ttttggatta ggccaaaatc ccttgcaggt ttcggctcag ggaatcatcg a
//
LOCUS       DAOTIL010000685         1670 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminobacteriaceae bacterium isolate AMR_MDS_3026
            NODE_86221_length_1670_cov_4.265015, whole genome shotgun sequence.
ACCESSION   DAOTIL010000685 DAOTIL010000000
VERSION     DAOTIL010000685.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868050
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminobacteriaceae bacterium (wastewater metagenome)
  ORGANISM  Aminobacteriaceae bacterium
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae.
REFERENCE   1  (bases 1 to 1670)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1670)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:19:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,224
            CDSs (total)                      :: 3,198
            Genes (coding)                    :: 3,184
            CDSs (with protein)               :: 3,184
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1670
                     /organism="Aminobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_86221_length_1670_cov_4.265015"
                     /isolate="AMR_MDS_3026"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:3034609"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..285)
                     /gene="rpsM"
                     /locus_tag="P5201_12185"
     CDS             complement(<1..285)
                     /gene="rpsM"
                     /locus_tag="P5201_12185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016285758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S13"
                     /protein_id="HRV99347.1"
                     /translation="MARIAGVDIPREKRVEIALTYIFGIGLPTSKKILAVTGVSPDTR
                     VKDLTEEESQKLRREIEDHYKVEGDLRREVSMNIKRLMDIGCYRGQRHKLG"
     gene            complement(292..417)
                     /gene="rpmJ"
                     /locus_tag="P5201_12190"
     CDS             complement(292..417)
                     /gene="rpmJ"
                     /locus_tag="P5201_12190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008523072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L36"
                     /protein_id="HRV99348.1"
                     /translation="MKVKPSVKPMCEFCRVIRRKGVVRIICSRNPRHKQRQGARR"
     gene            complement(529..750)
                     /gene="infA"
                     /locus_tag="P5201_12195"
     CDS             complement(529..750)
                     /gene="infA"
                     /locus_tag="P5201_12195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005661740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-1"
                     /protein_id="HRV99349.1"
                     /translation="MSNKDEVIEVRGKVIEPLPNAMFKVELDNGHKILAHVSGKMRLH
                     FIRILPGDRVLVEISPYDLSRGRIIYRYK"
     gene            complement(729..1154)
                     /locus_tag="P5201_12200"
     CDS             complement(729..1154)
                     /locus_tag="P5201_12200"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="KOW domain-containing RNA-binding protein"
                     /protein_id="HRV99350.1"
                     /translation="MGMGLNELSDGGMCTIGQVVRSRKGKDAGRWYVVVGVAEDCGPS
                     RRTQGARVLVADGKKFTVGKPKAKNMIHLQRTRWVLDEIAHSILSKGNFDAGRFQALL
                     TGLANKNSIGGSVGCDPGNTASGASSGEDEETACPIKTK"
     gene            complement(1139..>1670)
                     /gene="map"
                     /locus_tag="P5201_12205"
     CDS             complement(1139..>1670)
                     /gene="map"
                     /locus_tag="P5201_12205"
                     /EC_number="3.4.11.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013048050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="type I methionyl aminopeptidase"
                     /protein_id="HRV99351.1"
                     /translation="HGIPSRDRFLEEGDIISIDVGTCFSGYYGDAACTYPVGRVSPVR
                     EKLLSVTKKSLEEALLVAQAGRTLGDIGHAVERCVLESGYGIVRDYSGHGIGKYLHEP
                     PQIPNYGQPGRGITLKSGMTLAIEPMVMTGSEDVVTGSDQWVVKTADGSDAAHFEKSV
                     LVKDGDPEILTPWAWA"
BASE COUNT          336 a          510 c          394 g          430 t
ORIGIN      
        1 gccgagcttg tggcgctgtc ctctgtagca gccgatgtcc ataagacgtt tgatgttcat
       61 ggacacctct ctgcgaaggt cgccttccac cttgtagtga tcctcaatct ccctgcggag
      121 tttctgagac tcctcctcgg taagatcctt cacgcgcgtg tccggactta ctccggtgac
      181 ggcgagaatc ttcttcgagg tgggaaggcc gattccgaaa atatatgtca acgctatctc
      241 cacacgcttc tcgcggggaa tatcaactcc ggcgatacgg gccattcgcg cttacctcct
      301 tgcaccctgt ctctgcttat gccggggatt cctgctgcag ataatccgca cgacaccctt
      361 acgccggatt acccggcaaa actcgcacat cggctttacc gatggtttta ctttcattga
      421 ttccacgctc ctctgaaaga actgaaacag aggcccttca gggcttctga aaatatcttt
      481 tctccaagcg gtttattata caggagatcg gacaatatga aaatactctt atttatatct
      541 atagatgatt ctgcccctgg acaggtcgta cggagagatc tccaccagaa ccctgtctcc
      601 cgggaggatg cggataaaat gaaggcgcat ctttcccgaa acgtgggcga gtatcttgtg
      661 tccgttatcg agttccacct tgaacatcgc gtttggaagc ggctcgatta cttttcctcg
      721 tacttctatt acttcgtctt tattggacat gcggtctcct catcctcccc ggaggaggcg
      781 ccggacgctg tgtttcccgg atcgcagccg actgaccccc cgatactgtt cttatttgca
      841 agccccgtaa gcagagcctg aaaccttcct gcgtcaaagt tacctttcga caggatactg
      901 tgcgcaattt cgtcaaggac ccatcgcgtc cgctggaggt ggatcatgtt ttttgccttg
      961 ggtttcccga cagtgaactt ctttccgtcc gccaccaaaa ccctcgctcc ctgcgttctt
     1021 cgagatggcc cgcagtcctc tgcaacgccg actacgacgt accatcttcc ggcgtccttt
     1081 cctttccgag atctgaccac ttggccaatt gtgcacatcc cgccatcgga aagttcattc
     1141 aggcccatgc ccacggcgtc agtatctccg gatccccgtc tttgacgaga accgactttt
     1201 caaaatgagc agcatccgag ccgtcggccg ttttgacaac ccactggtcg cttccggtga
     1261 caacatcttc ggaacccgtc atcaccattg gttcaatggc cagcgtcatc ccggacttga
     1321 gggtgatccc tcttccgggc tgaccgtagt tcggtatctg gggcggttcg tgcagatact
     1381 tccctatacc gtgtcccgaa tagtcccgga caataccata accgctctcc aacacgcagc
     1441 gttccaccgc gtgtccgata tcgccgagcg ttctgccggc ctgcgcaacg agaagcgctt
     1501 cttcaagact cttcttcgtt acggagagaa gtttctccct caccgggctc actcttccca
     1561 cggggtaggt acatgcggcg tcgccgtaat atccggaaaa acaggttccg acatcaatac
     1621 tgatgatatc gccttcctcc aggaagcggt cgcggctggg tattccgtgg
//
LOCUS       DAOTIL010000686         1670 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminobacteriaceae bacterium isolate AMR_MDS_3026
            NODE_86231_length_1670_cov_3.627864, whole genome shotgun sequence.
ACCESSION   DAOTIL010000686 DAOTIL010000000
VERSION     DAOTIL010000686.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868050
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminobacteriaceae bacterium (wastewater metagenome)
  ORGANISM  Aminobacteriaceae bacterium
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae.
REFERENCE   1  (bases 1 to 1670)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1670)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:19:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,224
            CDSs (total)                      :: 3,198
            Genes (coding)                    :: 3,184
            CDSs (with protein)               :: 3,184
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1670
                     /organism="Aminobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_86231_length_1670_cov_3.627864"
                     /isolate="AMR_MDS_3026"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:3034609"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..238)
                     /locus_tag="P5201_12210"
     CDS             complement(<1..238)
                     /locus_tag="P5201_12210"
                     /inference="COORDINATES: protein motif:HMM:NF022321.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2703 domain-containing protein"
                     /protein_id="HRV99352.1"
                     /translation="MNRLIIRHFIPAADSGVRCVPCEETSGYLQSMMESLAPKLVNLD
                     IQLVLQSLEIHEITEANCGKLNYISFFAPELGLEV"
     gene            376..705
                     /locus_tag="P5201_12215"
     CDS             376..705
                     /locus_tag="P5201_12215"
                     /inference="COORDINATES: protein motif:HMM:NF018021.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallopeptidase family protein"
                     /protein_id="HRV99353.1"
                     /translation="MTYEDFCQVAEKMIENLPPKLFEQLNGGVNVVRQKKEDGEYVLM
                     GEYVEDPIMGNTVYLYYGTFAEELKGAKRSEWIEEIEETLLHELRHHVESLAGVDYLS
                     FEEMQDL"
     gene            845..930
                     /locus_tag="P5201_12220"
     tRNA            845..930
                     /locus_tag="P5201_12220"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:878..880,aa:Leu,seq:caa)
     gene            1409..>1670
                     /locus_tag="P5201_12225"
     CDS             1409..>1670
                     /locus_tag="P5201_12225"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRV99354.1"
                     /translation="MIKAASLFSQILSTIRTAANFDNLVAKHEAILRMGGVLLFVNLP
                     SDDAATEDVHDGFRQNVFCKRKILKKVKTFIGTSENALRIQIW"
BASE COUNT          475 a          382 c          425 g          388 t
ORIGIN      
        1 ccacttcaag ccccaattcc ggtgcgaaga aggaaatata gttcagtttg ccgcaatttg
       61 cttcggtgat ctcatggatc tccaggctct gaagcacaag ctggatatcc agattgacga
      121 gtttcggagc gagactctcc atcatcgact gaagataacc ggatgtttct tcgcagggga
      181 cgcagcgaac accggaatca gcagcgggta tgaaatgtcg tataatcagt ctgttcattc
      241 tcgatcgacc tccttgatgt gatccatgca ttacattata cactcggagg gtgttttaag
      301 ggaaaaggta tggagacgat tcctctctga aaacaaggag agcgtgatga agaaaaaaac
      361 aaggagaggt gcgcagtgac gtacgaagat ttttgccagg tagctgaaaa aatgattgaa
      421 aacctgcccc cgaagctctt cgagcagctc aacggaggcg tgaatgtcgt tcggcagaaa
      481 aaagaggacg gagagtatgt tcttatgggc gagtacgttg aagaccctat tatggggaat
      541 accgtctacc tttactatgg cacatttgca gaagaattaa aaggtgcgaa acgcagcgaa
      601 tggatcgagg aaatcgaaga gacgcttctc catgaactgc gccaccacgt ggaatctctg
      661 gccggtgtag actatctctc tttcgaggag atgcaggatc tgtagcgact tccccctaaa
      721 aaacactgga gggaaacgct atcttaaaat gaggaaaaca attcggttct tgttgtcgcc
      781 gcgcaaaatt gttcttaact ccgttgacat gtccgttttt ttcgattaag ataccgcagt
      841 gcatgccggg gtggcggaat ggtagacgca gcggactcaa aatccgccgt gggcgaccac
      901 gtgggggttc gagtcccccc cccggcacca atcattgaaa tgactggctt aaagagccga
      961 tttaaagtgt aaaagcattg ttttttatcc cctcaaaaat gacgaatccg tagtgatgta
     1021 aaacgagggg attacggcgg attttcgggg tcttctttca tcgtccgggc ggcgaaaagg
     1081 aggccccgaa atgttaggaa aaaatgaagc gccaaacagc tccgaaatca gatgggaaga
     1141 ggctttgcgc tattttctca ttttcaaggg agcggaggga agaagcgagc gtaccattca
     1201 ggattacgag agccacatca ggcgtttttt caaaaggtat ccggactctt tccagtccgg
     1261 accacctctt cggcaagccg ttctttcata tcttacggca ctcttgtcca aaataggttt
     1321 ctgaaaaatt aaaaagcccc ccggctttga gatatcattg gtttgccaac acaaaaagat
     1381 ctcaaaacaa aaaaaggggg caaaccaaat gataaaggca gcgagtctgt tcagtcaaat
     1441 actttcgacc atccgcaccg cagcgaactt tgacaacctg gtggccaaac acgaggcgat
     1501 ccttcgcatg ggcggcgttc tcctcttcgt gaacctccca tccgacgatg cagcgactga
     1561 agatgtccac gacggctttc gccagaatgt tttttgcaag aggaaaatcc tcaaaaaggt
     1621 gaagacgttc ataggaacga gcgagaacgc tcttcgcatc cagatttgga
//
LOCUS       DAOTIL010000687         1670 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminobacteriaceae bacterium isolate AMR_MDS_3026
            NODE_86232_length_1670_cov_3.627245, whole genome shotgun sequence.
ACCESSION   DAOTIL010000687 DAOTIL010000000
VERSION     DAOTIL010000687.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868050
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminobacteriaceae bacterium (wastewater metagenome)
  ORGANISM  Aminobacteriaceae bacterium
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae.
REFERENCE   1  (bases 1 to 1670)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1670)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:19:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,224
            CDSs (total)                      :: 3,198
            Genes (coding)                    :: 3,184
            CDSs (with protein)               :: 3,184
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1670
                     /organism="Aminobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_86232_length_1670_cov_3.627245"
                     /isolate="AMR_MDS_3026"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:3034609"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(270..1451)
                     /locus_tag="P5201_12230"
     CDS             complement(270..1451)
                     /locus_tag="P5201_12230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013048713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FprA family A-type flavoprotein"
                     /protein_id="HRV99355.1"
                     /translation="MKKRLMRDGIWMMGAVDWNRRLFDSLIPLPEGTSYNAYLVKGSE
                     KVALIDTADPELEHVLMEQLEDVERVDYIISLHTEQDHSGAIPTVLAKYPEARVVCST
                     KAKELLIDHIHLDESVIDAMPDGSTLSLGDRHFTFIHTPWVHWPETMCAYLHEERILF
                     SCDFFGSHLATSDMYAGESPLIYEGAKRYFAEIMMPFRSIVKSNIKKVEGYAIDIIAP
                     SHGPAFDKPEFIMNAYRDWTSDRVSNFVAIPYISMHGSTEIMVNRLLDALVERGVKVQ
                     KYELTVTDIGKLAIDLVDAATIVIGTPTVHFGPHPAVFSATHLANALRPKVKYAAIIG
                     SYGWGTKAVEQISGLIPNLKVEVLGTVMCKGLPREEDLKALDELADAIAAKHAPLMQA
                     E"
BASE COUNT          332 a          500 c          489 g          349 t
ORIGIN      
        1 caccagcacg aaatcctgcg ctccggcgaa agcgcacgaa gccgccagaa gcacgaacaa
       61 aaacgctgct gcacaccata ttcttttcat tgtgacaacc tgcccctctt tttacgagat
      121 aagcgccttc ctgcgaatga tagcagatgg cttcaaaaac acaagacaac ggcgcgcact
      181 acctgttttt ttcagcgttc aatatagcgg aggagaaatt tccgcaaacg ggcctctcct
      241 ccggtcagtt cctcggaaaa aactgccgtc tactccgcct gcatcagcgg ggcgtgcttc
      301 gcggcgatgg cgtcggccag ctcgtcgagc gccttcaggt cctcctcgcg cggaagtccc
      361 ttgcacatca cggttcccag cacctcgacc ttcagattgg ggatcaggcc ggagatctgt
      421 tccaccgcct tggtgcccca tccgtacgag ccgatgatgg ccgcgtactt caccttcggg
      481 cgcagcgcgt tcgccaggtg cgtcgcggag aagaccgcgg ggtgcggacc aaagtggacg
      541 gtcggcgtgc cgatgacgat ggtcgcggca tccacaaggt cgatggccag cttgccgatg
      601 tcggtgacgg taagctcgta cttctgcacc ttgacgccgc gctccacaag agcgtcgagg
      661 aggcggttca ccatgatctc ggtgctcccg tgcatcgaga tgtaggggat ggcgacaaag
      721 ttggacaccc tgtcggaggt ccagtcgcgg taggcgttca tgataaactc cggcttgtcg
      781 aaggccgggc cgtggctggg ggcgatgatg tcgattgcgt acccctccac cttcttgatg
      841 ttgctcttca cgatggagcg gaagggcatc atgatctcgg cgaagtagcg cttcgccccc
      901 tcgtagatca gcgggctctc accggcgtac atgtccgacg tggcgaggtg cgaaccgaag
      961 aagtcgcacg agaagaggat gcgctcctcg tgcaggtagg cgcacatcgt ctccggccag
     1021 tggacccacg gcgtatggat gaaggtgaag tgcctgtcgc cgagggagag agtggagccg
     1081 tcgggcatcg cgtcgatcac cgattcatcc aggtgaatat ggtcgatcag cagctccttc
     1141 gcctttgtgg agcagaccac ccgggcctcc gggtacttgg cgagcaccgt cgggatggcc
     1201 ccggagtggt cctgctcggt atgcagcgag atgatgtagt cgacccgctc cacgtcctcg
     1261 agctgttcca tcaggacatg ttcgagttcc gggtctgccg tgtcgatgag ggcgaccttc
     1321 tcgctcccct tcaccaggta cgcgttgtag ctcgttccct cgggaagcgg aatgagcgaa
     1381 tcgaagaggc ggcggttcca gtccaccgcg cccatcatcc agatgccgtc gcgcatgagt
     1441 ctctttttca ttgctgtatc tcctttcgtc cttcgctgta aaatctctac gaccgatcgc
     1501 gtgtccggtc gtgaaatact cctcaagcgt ataaattata tcaataaaac tttttaaggg
     1561 caagaatacg cgcacttttt cctccggaca ggacagctcc gagtccggag gaaaaacaca
     1621 acaacattcc tgcgcgctcc ttagcgccgg caggccgggg aggcctattc
//
LOCUS       DAOTIL010000688         1668 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminobacteriaceae bacterium isolate AMR_MDS_3026
            NODE_86387_length_1668_cov_2.877867, whole genome shotgun sequence.
ACCESSION   DAOTIL010000688 DAOTIL010000000
VERSION     DAOTIL010000688.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868050
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminobacteriaceae bacterium (wastewater metagenome)
  ORGANISM  Aminobacteriaceae bacterium
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae.
REFERENCE   1  (bases 1 to 1668)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1668)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:19:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,224
            CDSs (total)                      :: 3,198
            Genes (coding)                    :: 3,184
            CDSs (with protein)               :: 3,184
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1668
                     /organism="Aminobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_86387_length_1668_cov_2.877867"
                     /isolate="AMR_MDS_3026"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:3034609"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1223
                     /locus_tag="P5201_12235"
     CDS             <1..1223
                     /locus_tag="P5201_12235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006300428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="YifB family Mg chelatase-like AAA ATPase"
                     /protein_id="HRV99356.1"
                     /translation="SVPRGIFLGELALDGRLRGVRGAVGAAILARDSGVPLFAPAENA
                     PEVSLVKGCEAYAVEDLADLFAFFRGELELPSVPEYAPQEEDVRADPDFADIRGQAGA
                     RRALEIAAAGHHNILLIGSPGSGKTLLARALRGILPPLSHEELLETTLVRSTLGLPFE
                     RGVQPPFRSVHHTASTVSICGGGATLRPGEISLAHRGVLFLDEFPEFRRDLLEGLRQP
                     LEDGAITVSRAAGSALYPCRVLLVVAANPCQCGWDGDPVERCVCSHLERERYRRKISG
                     PILDRIDLHVSVPRLLPEELVAVESAGGECSADVAKRVRAAREMQRERWAPHGFSCNA
                     ELPERLVKRSLALSAGVRPFLSSMAGSLRLSGRGIGRVLKVARTIADLARSESVEVPH
                     VAEALAYRDGGVWR"
     gene            1220..>1668
                     /locus_tag="P5201_12240"
     CDS             1220..>1668
                     /locus_tag="P5201_12240"
                     /inference="COORDINATES: protein motif:HMM:NF014533.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009294 - DNA-mediated transformation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-processing protein DprA"
                     /protein_id="HRV99357.1"
                     /translation="MNPVLRTLLLLAGSGAPAVRIWNAFESEALPPEAFWEEGEVLWC
                     RLGCTEAVCRMLRRLADSSWPDEEELRAKQAGVRLLTFRDKEWPDALGGQPDSPRLLY
                     VKGKWPLRGPSVAVVGTRRCSAYGMRAAFEIGRAVGGAGGVVISGGAA"
BASE COUNT          244 a          550 c          575 g          299 t
ORIGIN      
        1 cctccgttcc cagggggata tttctcggag aactcgctct tgacggacgt cttcggggag
       61 tccggggcgc cgtgggcgct gcgatcctcg cccgcgactc gggagtcccc ctcttcgcgc
      121 ccgctgaaaa cgccccggag gtatcgctcg tcaaggggtg cgaagcgtat gctgtagagg
      181 accttgccga tctcttcgcc ttcttccggg gcgaattgga gcttccctcg gtaccggagt
      241 acgcgcccca agaagaggac gtccgcgccg accccgactt cgccgatatc agggggcagg
      301 cgggggcgcg ccgcgcgctg gagatcgcgg ccgctgggca ccataacatc ttgctcatcg
      361 gttcgccggg aagcggaaag acgctccttg cacgggcgct tcgcggcatc ctcccgccgc
      421 tctcccacga ggagctgctg gaaacaacgc tggtgcggag tacgctcggc ctcccctttg
      481 aacggggggt acagccgccg ttccggtcgg tccatcacac ggcgagcacc gtctccatct
      541 gcggcggcgg cgcgaccctt cgccccggag aaatcagtct cgcccacaga ggggtgctct
      601 tcctcgacga gtttcccgag ttccggcgcg atctgctcga aggtctccgt cagccgctgg
      661 aggacggagc gatcacggtc agcagggccg cgggatccgc gctttacccc tgccgcgtgc
      721 tgcttgtcgt cgccgcaaac ccctgtcagt gcggctggga cggcgatccc gtggaacgat
      781 gcgtctgctc ccacctggag agggagcgct accgccgcaa gatttccgga cctatcctcg
      841 acagaatcga cctgcacgtc tccgtaccgc ggctcttgcc ggaagagctc gtcgcggtgg
      901 agagcgcggg cggcgaatgc agcgcggacg tcgcgaagcg tgtgcgtgcc gcgcgggaga
      961 tgcagaggga gcggtgggcc cctcacggct tttcctgcaa cgccgagctt cccgaacgcc
     1021 tggtcaaacg ctcgcttgct ctttcggccg gcgtgcgccc cttcctctct tcgatggcgg
     1081 gaagcctgcg cctctccgga agaggtatag gacgggtgct caaggtcgcg cggacgatcg
     1141 cggatcttgc gcgaagcgaa agcgttgaag tccctcacgt cgcggaagcc ctcgcgtaca
     1201 gggatggagg ggtctggaga tgaaccctgt gctccggacg ctcctgctgc ttgcgggctc
     1261 gggtgctcct gccgttcgta tctggaacgc attcgagtcg gaggctcttc ctccggaggc
     1321 gttctgggag gagggggaag ttctctggtg tcgccttggg tgtaccgaag ctgtgtgcag
     1381 gatgctgcgt cgcctcgcgg actcttcctg gcccgacgaa gaggaactgc gcgcgaagca
     1441 ggccggcgtc cgtcttctca ccttccgcga caaggagtgg cccgacgcgc tcggcgggca
     1501 gcccgactcc ccgcggctgc tctacgtcaa aggcaagtgg ccgctccgcg gcccttccgt
     1561 tgccgtcgtg gggaccagac gatgctcggc gtacggtatg cgcgccgcat tcgagatagg
     1621 gcgggccgtc ggcggcgcag ggggcgtcgt catcagcggc ggagcggc
//
LOCUS       DAOTIL010000689         1656 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminobacteriaceae bacterium isolate AMR_MDS_3026
            NODE_87260_length_1656_cov_3.249844, whole genome shotgun sequence.
ACCESSION   DAOTIL010000689 DAOTIL010000000
VERSION     DAOTIL010000689.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868050
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminobacteriaceae bacterium (wastewater metagenome)
  ORGANISM  Aminobacteriaceae bacterium
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae.
REFERENCE   1  (bases 1 to 1656)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1656)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:19:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,224
            CDSs (total)                      :: 3,198
            Genes (coding)                    :: 3,184
            CDSs (with protein)               :: 3,184
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1656
                     /organism="Aminobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_87260_length_1656_cov_3.249844"
                     /isolate="AMR_MDS_3026"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:3034609"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..326
                     /locus_tag="P5201_12245"
     CDS             <1..326
                     /locus_tag="P5201_12245"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRV99358.1"
                     /translation="MGKLAQAGANGAVMAAAAGMYGFVQFRKSADLEGLRDIIPYSFE
                     FHGKCHYVGEDLVEASIPYERIVPVIAASDFEGYIMTEFENEGDYDEVEMTRRNIAMV
                     KKYAR"
     gene            583..>1656
                     /locus_tag="P5201_12250"
     CDS             583..>1656
                     /locus_tag="P5201_12250"
                     /inference="COORDINATES: protein motif:HMM:NF013068.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS256 family transposase"
                     /protein_id="HRV99359.1"
                     /translation="MGRQKITPERAAFRKQLGELMARAGINSMKDIEGLFQEMIGSFV
                     ENGLEGELEEELGYSKYDYRNKETDNSRNGHSEKTLRTSYGDVEIQIPRDRKGEFEPE
                     LVKKQQTTLSGDIEEKILSMYAKGMTTGDIEGHIREIYGLSVSDSTISRITDKILPVV
                     REWQARPLETIYAVVHMDAIHFHVRSEGQVVKKAVYIVLGLDMKGRRDVLGMYIGENE
                     SAKFWLTVLNNLKNRGVEDILIACVDGLTGFPAAIEAAYPRTEVQKCVIHQIRNTTQF
                     VSYKDIKALMADLKRVYTAVDEATALSELERFEELWGKKYPKIGKSWRANWAGLSTYF
                     KYPAEIRKIIYTTNTVEGFNRQLR"
BASE COUNT          416 a          405 c          553 g          282 t
ORIGIN      
        1 ccatgggaaa gcttgcgcag gcgggcgcga acggcgcggt gatggccgcg gcggcgggca
       61 tgtacggctt cgtgcagttc cggaagagcg cggacctcga aggcctcagg gacatcatcc
      121 cttacagctt cgaattccac ggaaagtgcc actacgtcgg cgaggacctc gtcgaggcga
      181 gcatccccta tgagcggatc gtgcccgtga tcgcggcctc cgacttcgag ggctacatca
      241 tgaccgagtt tgaaaatgag ggcgattacg acgaagtgga gatgacgcgg cgcaacatcg
      301 cgatggtcaa aaaatacgcc cgctgaaatc gtaagcgtgg tggaacaaat tcgtttggat
      361 ccgcagtcgg gcagcctttt tctgttccgt gggaagagac gggtttgttc ggttgcgcag
      421 gcggccggcg gatgagaatt accgatgtcc acggacacag acgcgggagg tgagtcttca
      481 gcagtcccgc tggcgcatgg agggactgag cgcgagaccg tcccaaatta tgtgtaaacg
      541 ttcaagctgt ggtagaatga aacagcgtgg aggtttacaa gaatgggaag acagaaaatc
      601 acaccggaac gggcggcatt tcgaaagcaa ctcggcgagc tcatggcaag agcagggatc
      661 aattctatga aggatatcga gggactcttt caggaaatga tcggcagttt cgtggaaaat
      721 gggctggagg gcgaactgga agaggagctc gggtactcga agtacgatta ccgcaacaaa
      781 gagacggaca acagccggaa cgggcacagc gagaaaacgc tcaggacaag ctacggggac
      841 gtggagatac agataccccg cgaccggaag ggcgagttcg agccggagct ggtgaagaag
      901 cagcaaacaa cgctcagcgg cgacatcgaa gagaaaatcc tgtcgatgta cgcgaaaggg
      961 atgacgaccg gggacatcga ggggcacatc cgggaaatct acggcctttc ggtatcggac
     1021 agtacgatca gccggatcac ggacaagata ctgcccgtgg ttcgggaatg gcaggcgcgg
     1081 ccgctggaaa cgatctacgc agtggtgcac atggacgcga tccacttcca tgtgcgatcg
     1141 gaaggacagg tggtgaaaaa ggcggtgtac atcgttctgg gcctggacat gaagggacgg
     1201 cgagacgtac tggggatgta catcggggag aatgagagcg cgaagttctg gctgacggtg
     1261 ctcaataacc tcaagaaccg cggggtggaa gacattctca tcgcctgcgt ggacggactg
     1321 acgggctttc ccgcggcgat tgaggcggcc tacccccgga cggaggttca gaagtgcgtg
     1381 atccaccaaa tccgcaatac cacgcagttc gtctcctata aggacatcaa ggcgctcatg
     1441 gcggacctga agcgcgtcta cacggcagtg gacgaggcga ccgcactttc ggagcttgag
     1501 cggtttgagg agctgtgggg gaagaaatat cccaagatcg ggaaatcctg gcgggcgaat
     1561 tgggcggggc tgagcacgta tttcaagtac cccgccgaga ttcggaagat catctacacg
     1621 accaacacgg tcgagggctt taatcgacag ctccgg
//
LOCUS       DAOTIL010000690         1646 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminobacteriaceae bacterium isolate AMR_MDS_3026
            NODE_87973_length_1646_cov_4.621622, whole genome shotgun sequence.
ACCESSION   DAOTIL010000690 DAOTIL010000000
VERSION     DAOTIL010000690.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868050
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminobacteriaceae bacterium (wastewater metagenome)
  ORGANISM  Aminobacteriaceae bacterium
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae.
REFERENCE   1  (bases 1 to 1646)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1646)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:19:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,224
            CDSs (total)                      :: 3,198
            Genes (coding)                    :: 3,184
            CDSs (with protein)               :: 3,184
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1646
                     /organism="Aminobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_87973_length_1646_cov_4.621622"
                     /isolate="AMR_MDS_3026"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:3034609"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..952)
                     /gene="ftsA"
                     /locus_tag="P5201_12255"
     CDS             complement(<1..952)
                     /gene="ftsA"
                     /locus_tag="P5201_12255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013048222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0043093 - FtsZ-dependent cytokinesis
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsA"
                     /protein_id="HRV99360.1"
                     /translation="MYKEPEILVGLDLGTSKISVVIAERDSRFQEAQIIGVGYAPSQG
                     IRKGLIINLEQAVRSVRQAIKDAENMVGFEIDEATVSFSGMEVASVTSRGMISLGRTP
                     RQVSISDVERVIEAAQSELSIPPNRVTLHTIPVKYSIDGNSGIDDPLGMTGIRLEMEL
                     QSVIVPTTVVQNIINCVESAGIEVNGLVIKSLASALGSLTDEETRAGVISLCIGGGTT
                     GVALYTDGRPVKLSIIPIGGDHITNDLAYVMKIPISKAEEVKKMVSLNPEEIEQQDLD
                     VDVRGRMKTFSIGEAAEVVNCRLEELFGEHVRHQLAEFSLH"
     gene            complement(953..>1646)
                     /locus_tag="P5201_12260"
     CDS             complement(953..>1646)
                     /locus_tag="P5201_12260"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRV99361.1"
                     /translation="IESEDPVAELFFWKNISSRALRFWPAFFLEKDDLRKAVEIQAPL
                     FCTISRRGITGVEVQIEPLRPWIVAQWNKREYYVSQDGFAWDTANELNKMIRGIVRPN
                     APLITFSDDFPSPALENSSAIVSRVVFPLELLQGWLDGLAENRWGARVEQISVSRREG
                     KFLLKLVFDKGGAHVLLWGDRPRWKELSSALSQIMEQLHFLGNNIIIDTTYTDRIIVR
                     GMARGDQEGSGR"
BASE COUNT          420 a          440 c          351 g          435 t
ORIGIN      
        1 tgtgcagact gaactcggcg agctgatggc gaacgtgttc accaaaaagc tcttccaatc
       61 tgcagttcac tacctcggcg gcttcaccaa tgctgaaagt cttcattcgt ccgcgaacgt
      121 cgacgtcaag atcctgttgc tctatttcct cgggattgag agagaccatc tttttaacct
      181 cttccgcctt actgatcgga attttcataa cgtacgcgag gtcgttcgta atatgatctc
      241 cgcctatcgg gataatcgac agtttaacag ggcgtccgtc ggtatagagg gcgactccgg
      301 tagttcctcc tccgatgcac aacgatatca ccccggcgcg tgtctcttcg tcggttaagg
      361 atccaagagc actggcaagt gattttatta ccagtccatt gacctcaata ccagcgcttt
      421 cgacgcagtt gattatattt tggaccacag ttgtcggaac aattaccgat tgcaattcca
      481 tttcgagacg aatgcccgtc atacctagag gatcgtctat tccggaatta ccgtcgattg
      541 agtatttaac aggaatggtg tgcaatgtga cacgattcgg gggtattgag agctcgctct
      601 gcgcagcttc aataacccgt tccacatcgg agatagagac ctggcgaggc gttcttccaa
      661 gagaaatcat ccctcttgaa gttaccgaag caacttccat cccgctgaaa gaaacggttg
      721 cttcgtctat ttcaaaacca accatgttct cagcgtcttt tattgcttga cgtacggatc
      781 gcacagcctg ttcaagattt atgatcagtc cttttcggat gccttgggac ggcgcgtacc
      841 ccacgccaat aatttgagct tcctggaaac gggaatccct ctctgcaata acgacggata
      901 ttttactcgt accaagatcc agcccaacca aaatttccgg ctctttgtac atttatctcc
      961 cgctcccttc ctgatcgccg cgcgccatgc cccggacgat aattctgtcg gtatatgtgg
     1021 tatctattat aatgttgttg ccgagaaaat gtaactgctc cattatctgc gaaagcgcgg
     1081 atgaaagctc tttccaacgg ggacggtctc cccataaaag aacgtgcgct cctcctttat
     1141 cgaaaacgag ttttaataaa aactttcctt ctctcctcga aacgcttatt tgctcaactc
     1201 gagcgcccca cctgttttct gccaagccat ccaaccaacc ctggagaagc tccagcggga
     1261 aaactactct ggaaacaata gcagaagagt tttccaaagc aggagaaggg aaatcgtcag
     1321 aaaaagttat caaaggtgca ttgggacgca caatacccct gatcattttg ttcagttcat
     1381 ttgcggtgtc ccaagcaaaa ccgtcttgag aaacataata ctcgcgtttg ttccattgag
     1441 ctacaatcca aggtcgtagc ggctctatct gaacttcaac tcccgtgatt ccccttcggc
     1501 ttatcgtaca aaagagagga gcctgtatct cgactgcctt tcgcaaatca tctttttcca
     1561 agaaaaacgc gggccagaat cgcaacgcgc gcgaactgat attcttccag aaaaacaact
     1621 ccgctacggg gtcctcagat tcaata
//
LOCUS       DAOTIL010000691         1641 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminobacteriaceae bacterium isolate AMR_MDS_3026
            NODE_88384_length_1641_cov_3.692938, whole genome shotgun sequence.
ACCESSION   DAOTIL010000691 DAOTIL010000000
VERSION     DAOTIL010000691.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868050
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminobacteriaceae bacterium (wastewater metagenome)
  ORGANISM  Aminobacteriaceae bacterium
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae.
REFERENCE   1  (bases 1 to 1641)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1641)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:19:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,224
            CDSs (total)                      :: 3,198
            Genes (coding)                    :: 3,184
            CDSs (with protein)               :: 3,184
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1641
                     /organism="Aminobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_88384_length_1641_cov_3.692938"
                     /isolate="AMR_MDS_3026"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:3034609"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..224
                     /locus_tag="P5201_12265"
     CDS             <1..224
                     /locus_tag="P5201_12265"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRV99362.1"
                     /translation="AFGMYPPGAEVVLSDGRSGVVVAPGERGVLRPRVLLHRDERGDE
                     LSSAVFLDLAAAPALFIRRCAGEDVPEAV"
     gene            complement(228..1220)
                     /locus_tag="P5201_12270"
     CDS             complement(228..1220)
                     /locus_tag="P5201_12270"
                     /inference="COORDINATES: protein motif:HMM:NF016114.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0030246 - carbohydrate binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar-binding transcriptional regulator"
                     /protein_id="HRV99363.1"
                     /translation="MQERPRDENSPRRQYLAEVAHLYYNQGKTQQEIAQLVGITRSGV
                     SRLLTEAREKGIVEILVHYPWQTSKALEDELSELFGLKDVRVLVRGGKNDREMLDGLG
                     SVAAQFFLNILRDGHIVGVSWGTALYSMLQAIRPVHRPDVKVVQLIGGTGAEHSSIVG
                     PLLAPMLANLLGCSCHYLHAPLITESEIFRKAVLQERPIRETLELGRKANIALVGIGA
                     THPEIYNPFRLGYISKDELNEISGNGAVGLICGRHYSIDGTELDIQVNRKVIGITLKE
                     LEEVEWVIGVAGGALKAESILGALRGRHVNVLVTDDIAARKALEIHKAGGSRAV"
     gene            1426..>1641
                     /locus_tag="P5201_12275"
     CDS             1426..>1641
                     /locus_tag="P5201_12275"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="involved in autoinducer 2 transport and processing;
                     Derived by automated computational analysis using gene
                     prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxy-5-phosphonooxypentane-2,4-dione
                     thiolase LsrF"
                     /protein_id="HRV99364.1"
                     /translation="MFEHMSWGKKNRLSSIVAPDGRALMLAIDHGYFMGATHGMEKPA
                     EAIEKLLPHVDSLMLSPGILSTCIDPAL"
BASE COUNT          300 a          521 c          474 g          346 t
ORIGIN      
        1 gcgctttcgg gatgtacccg cccggcgccg aggttgtcct ctccgacgga cggtccggag
       61 tcgtcgttgc cccgggagaa cggggggtgc tccgccccag ggtacttctg catcgcgacg
      121 agcgaggaga cgagctctct tccgccgtct ttctcgacct tgcggccgcg cccgcgctct
      181 ttatacgaag atgcgccgga gaggacgttc ccgaggccgt ctgatcctta aactgcccgc
      241 gatcccccgg ccttgtggat ctcaagcgcc tttcgcgcgg cgatgtcgtc ggtgacgagg
      301 acgttcacgt gccgtcccct gagcgctccc aggatcgact ccgccttgag cgcgcccccc
      361 gcgacgccga tcacccactc aacctcttcc agctccttca gcgtaatgcc gatcactttt
      421 ctgttcacct gaatatccag ctccgtgccg tcgatggagt agtgacggcc gcagatgagc
      481 ccgaccgcgc cgttgccgga gatctcgttc agctcgtcct tggaaatgta ccccaggcgg
      541 aaggggttgt agatctccgg gtgcgtcgcg cctatgccca ccagggcgat gtttgctttt
      601 cgtccgagtt cgagcgtctc gcggatcggg cgttcctgaa ggaccgcctt gcgaaatatc
      661 tcgctctccg tgatcagcgg cgcgtgaagg tagtggcacg aacagccgag gaggttcgcc
      721 agcatcggcg cgagcagcgg gccgacgatg gacgagtgtt cggcccccgt gcctccgata
      781 agctgcacca ccttgacgtc cggccggtgc acggggcgga tcgcctggag catgctgtag
      841 agggccgttc cccacgagac gccgacgatg tggccgtccc gcagaatgtt caggaagaac
      901 tgcgccgcga cggaacccag tccgtcgagc atctcgcggt cgttcttccc tccccggacg
      961 aggacgcgga cgtccttcag cccgaaaagc tccgaaagct cgtcctcaag cgctttcgaa
     1021 gtctgccacg gatagtggac gaggatctcg acgatcccct tctcgcgtgc ctccgtcaac
     1081 agccgcgaaa cccccgaccg cgtgatgccc acgagctgcg cgatctcctg ctgcgtcttc
     1141 ccctggttat agtacagatg agccacctcg gcgagatact gccttctcgg gctgttctcg
     1201 tccctcgggc gttcctgcat gctcttcccc tcctcggatg tctctccgtg tatagcgctg
     1261 cattgtacca gaatagagca gaataaaagt gaaaagacgt ctcaagaaat ttgtagtttt
     1321 tttcttgaca atacagggac atacgttaga atgacatgca gagtatatca ctttcgtgat
     1381 tgaatttcac atacgtgaaa tctttttgtc acaaggagga acgctatgtt tgagcatatg
     1441 agctggggaa agaagaacag actgagttcc atcgtggctc ccgacggcag agcgctgatg
     1501 ctcgccatcg accacggcta tttcatgggc gccacgcacg gcatggagaa gccggccgag
     1561 gcgatagaga agctgcttcc gcacgtcgat tccctgatgc tgagcccggg aattctttcc
     1621 acctgcatcg atcccgccct g
//
LOCUS       DAOTIL010000692         1641 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminobacteriaceae bacterium isolate AMR_MDS_3026
            NODE_88395_length_1641_cov_3.217528, whole genome shotgun sequence.
ACCESSION   DAOTIL010000692 DAOTIL010000000
VERSION     DAOTIL010000692.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868050
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminobacteriaceae bacterium (wastewater metagenome)
  ORGANISM  Aminobacteriaceae bacterium
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae.
REFERENCE   1  (bases 1 to 1641)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1641)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:19:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,224
            CDSs (total)                      :: 3,198
            Genes (coding)                    :: 3,184
            CDSs (with protein)               :: 3,184
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1641
                     /organism="Aminobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_88395_length_1641_cov_3.217528"
                     /isolate="AMR_MDS_3026"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:3034609"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..923)
                     /locus_tag="P5201_12280"
     CDS             complement(<1..923)
                     /locus_tag="P5201_12280"
                     /inference="COORDINATES: protein motif:HMM:NF015948.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF362 domain-containing protein"
                     /protein_id="HRV99365.1"
                     /translation="MSATTFTQCGKYSRLDIEKALSALLQEFGSMKNIISPGDSVLLK
                     ANMLSASTPEECITTHPEVVRAVAVQTLEAGGKPFIADSPGIDRFSDVARKTGMEAVA
                     RELGIPCVELDDPVPLPVAEGNVFQRIEVSRRAVEADTIINIPKLKTHAQMMLTLGVK
                     NLFGTVVSQRKAQWHYNVGLRREAFADLHLDIARGLAPRLTILDGIRGMEGRGPSNGK
                     PRDFGILAASSDPLDLDFRVCTLLGAPLEEFPLYRAARERGLVDKSARWDAPGLPEEF
                     EIPPIELPKLDALHLLPPFLDFFLLFQCQFNT"
     gene            1015..>1641
                     /locus_tag="P5201_12285"
     CDS             1015..>1641
                     /locus_tag="P5201_12285"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRV99366.1"
                     /translation="MAYNVQQFFLQKRGVGYVAPILCTMRGLGESDIEQAVVFHKRIS
                     DEIGDADFFAEENTIAESIMGEGVVVGVFAEGNLIALRAVSYVKEFVDDALIDLDLDP
                     GEADNVAVMDFCVTDSAFRGNSIQFFTYLFTESLMYPNRYHLHTTVSPKNIFSLTNVL
                     KCGFSAINFKQKYGGHHRFVLYKNLRKPGAVKTRGHKEILLRNYDYHPK"
BASE COUNT          341 a          475 c          480 g          345 t
ORIGIN      
        1 gtattgaact ggcattgaaa aagcagaaag aaatcgagga agggggggag caggtggagc
       61 gcgtcgagct tgggcagctc tatcggtgga atctcgaact cctccggaag accgggggcg
      121 tcccacctcg cacttttgtc cacgagtcca cgctcccgcg cagccctgta gagagggaat
      181 tcttccaacg gagcccccag cagcgtacag acgcggaagt caaggtcgag cgggtccgac
      241 gaggccgcaa ggatgccgaa atctctcggc ttcccgttgc ttggcccccg tccctccatg
      301 ccccggattc cgtcgagtat ggtgaggcgt ggggcgaggc cgcgcgcgat atccaggtgg
      361 agatcggcga acgcctcccg ccgaagcccg acgttatagt gccactgcgc ctttcgctgg
      421 ctgaccaccg tgccgaaaag atttttcact cccagtgtga gcatcatctg cgcgtgcgtc
      481 ttgagcttcg ggatgttgat gatcgtatcg gcttcgacgg cgcgccgcga tacctcaatg
      541 cgctggaaga cgtttccctc cgcgacgggc agcggaacgg ggtcgtccag ctcgacgcac
      601 ggaatgccca gttcgcgtgc gacagcctcc atcccggtct tcctcgcgac gtcggagaaa
      661 cggtctattc cggggctgtc tgcgatgaac ggtttccctc ctgcctcaag cgtctgcacc
      721 gccacggcgc gtacgacctc cgggtgcgtc gtgatgcact cctcgggcgt cgaggcggaa
      781 agcatattcg ccttcagcag gacgctgtcc cccggggata taatgttctt catactcccg
      841 aactcttgca gcagcgccga gagggccttc tcgatatcca atcggctgta ttttccgcac
      901 tgtgtgaatg ttgttgcgct caccatcttc tctcctctcg attcgttttg tataatatac
      961 accgaaacag caaacgatga agtgcatcca cacatgggaa gggccggtga atcgatggct
     1021 tacaacgtcc agcagttctt tcttcaaaaa agaggcgtgg ggtacgtcgc ccccattctg
     1081 tgcaccatgc ggggcttggg ggagagcgac atagagcagg ccgtcgtctt ccataaaagg
     1141 atatccgacg agatcggcga cgcggatttt ttcgccgagg aaaacacgat cgcggagagc
     1201 atcatggggg aaggcgtcgt cgtcggggtc tttgcggagg gaaatctcat cgcgcttcgg
     1261 gccgtttcct atgtcaaaga gtttgtggac gacgcgctca ttgatctcga tctggacccc
     1321 ggcgaagcgg acaacgtcgc cgtgatggat ttttgcgtca ccgattccgc cttcagaggg
     1381 aacagcattc agttctttac gtacctcttc accgagagtc tcatgtatcc gaacagatac
     1441 catctgcaca ccacggtgtc gccgaagaat atcttcagcc tgacgaacgt gctgaagtgc
     1501 ggtttttccg cgatcaattt caagcagaag tacggcgggc atcaccgttt cgtgctctac
     1561 aagaacctga ggaaacccgg cgcggtgaag acgcgggggc acaaggagat actcctcaga
     1621 aactacgact accatccgaa g
//
LOCUS       DAOTIL010000693         1640 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminobacteriaceae bacterium isolate AMR_MDS_3026
            NODE_88467_length_1640_cov_3.791167, whole genome shotgun sequence.
ACCESSION   DAOTIL010000693 DAOTIL010000000
VERSION     DAOTIL010000693.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868050
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminobacteriaceae bacterium (wastewater metagenome)
  ORGANISM  Aminobacteriaceae bacterium
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae.
REFERENCE   1  (bases 1 to 1640)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1640)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:19:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,224
            CDSs (total)                      :: 3,198
            Genes (coding)                    :: 3,184
            CDSs (with protein)               :: 3,184
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1640
                     /organism="Aminobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_88467_length_1640_cov_3.791167"
                     /isolate="AMR_MDS_3026"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:3034609"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1533)
                     /locus_tag="P5201_12290"
     CDS             complement(<1..1533)
                     /locus_tag="P5201_12290"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRV99367.1"
                     /translation="MRETENRLPRSETTFVFPQRLSARLDGISAFPVTIVEAPSGFGK
                     TTAVREFLKSLQHNSRQSWYTCFGEHPSKVWEGVCRLFWNVDIDVARRLKELFPPTVE
                     TLPDIAELMRECRCDAATFLVIDNYQLFESPIPYHIAHAFSAHSNPNLHMLFITQPLP
                     TFEIAVQDANIHRLGTRDFFFDRENTARLCRTAGARMTEKEIDRIQSVSEGWVSAILL
                     QASNYIETGAFADARDMDALVETAIWNRMTGEEHEFLLGLSLLDAFSPRQAAIMAGGP
                     TLPEHLLKMLKNGFFISYIADKGVYSLHSILNDYLKQRFENQPREFAEIMIRRAGAAC
                     AAASDYFPAAQFFSRVRDYGAILSMPLTTSYLNEQKEKDIVGFLERFVGECPEQTLQE
                     YPFALLTFAFQFMKSGKRALFSQMVRLLRAIADAPSGLPESTVSRIRGETALLLSFTE
                     FNDIAKMSAYHREALLHLNMSEDADPPKTVVFGSTPWTFGVASVLCLYWSKSGELDAE
                     LAL"
BASE COUNT          349 a          442 c          477 g          372 t
ORIGIN      
        1 cagcgccagt tccgcatcaa gttccccgct cttgctccag tagaggcaga gcaccgacgc
       61 gacgccgaac gtccacggcg tcgagccgaa cacgacggtt ttcgggggat cggcgtcctc
      121 ggacatgttc aaatggagaa gggcctctct atggtatgcg ctcattttcg cgatatcgtt
      181 gaactctgta aaggagagca gcagcgccgt ttcccccctg atacgcgaca ccgtcgattc
      241 cgggagcccg gagggagcgt ccgcaatcgc gcgcaacagg cggaccatct gcgaaaagag
      301 cgcccttttg ccgctcttca tgaactgaaa cgcgaaggtg aggagggcga acggatattc
      361 ttgaagagtc tgttccgggc attctcctac gaatcgttca agaaagccga cgatgtcttt
      421 ttccttctgt tcgttcaaat aacttgtcgt gagcggcatt gagagaatgg cgccgtagtc
      481 ccgaaccctg gagaaaaact gcgcggccgg aaagtagtcc gaagcggcgg cgcaggctgc
      541 gcccgccctg cgaatcatga tctccgcaaa ttcgcggggt tggttctcga agcgctgttt
      601 cagatagtcg ttcaggatgc tgtggagaga atagacgcct ttgtccgcga tgtacgagat
      661 aaaaaaaccg tttttcagca tcttgagcag gtgttccggc aaggtcgggc cccctgccat
      721 gatagccgcc tgtctggggg agaaagcgtc gaggagagag agcccaagga ggaattcatg
      781 ttcttccccg gtcattctgt tccatatcgc cgtttcgacg agcgcgtcca tgtcgcgcgc
      841 gtcggcgaac gcccccgttt caatgtagtt tgaggcctgg agcaggatgg cggagaccca
      901 tccttcggag acgctctgaa tccggtcgat ttccttctcc gtcatgcgtg ctccggccgt
      961 acggcagagt cgcgccgtat tctcgcggtc gaagaagaaa tctctcgttc ccagtctgtg
     1021 tatgtttgcg tcctgaaccg cgatttcaaa agtcggaaga ggctgggtta tgaagagcat
     1081 gtggagattc gggtttgaat gcgcggaaaa ggcgtgcgca atatggtacg ggatcggact
     1141 ttcaaacagt tgatagttgt cgatcacaag aaacgtcgcg gcgtcgcagc ggcactcgcg
     1201 catcagctcg gcgatatcgg gaagcgtttc gaccgtcggg gggaacaact ccttcagtct
     1261 tcgcgcaacg tcgatatcca cattccagaa cagtctgcac acaccttccc acaccttcga
     1321 ggggtgttcg ccaaagcatg tgtaccagct ctgtctgctg ttgtgctgaa gactctttaa
     1381 aaattctctg acggctgtcg tcttgccgaa gccggacggc gcttccacta tggtgaccgg
     1441 gaacgcggat attccgtcca ggcgtgccga caggcgttga gggaagacga acgtcgtctc
     1501 gctacggggt aatcggtttt ctgtctctcg catggcgtct ctctccgttc atcccgatag
     1561 gaaattcaat actttttgga gacgctccgg ctgtaatgtc ggaacgtctc caaaaagttt
     1621 acagcaaaga ctgggaatgt
//
LOCUS       DAOTIL010000694         1639 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminobacteriaceae bacterium isolate AMR_MDS_3026
            NODE_88549_length_1639_cov_3.774621, whole genome shotgun sequence.
ACCESSION   DAOTIL010000694 DAOTIL010000000
VERSION     DAOTIL010000694.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868050
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminobacteriaceae bacterium (wastewater metagenome)
  ORGANISM  Aminobacteriaceae bacterium
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae.
REFERENCE   1  (bases 1 to 1639)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1639)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:19:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,224
            CDSs (total)                      :: 3,198
            Genes (coding)                    :: 3,184
            CDSs (with protein)               :: 3,184
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1639
                     /organism="Aminobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_88549_length_1639_cov_3.774621"
                     /isolate="AMR_MDS_3026"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:3034609"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(47..517)
                     /gene="nuoE"
                     /locus_tag="P5201_12295"
     CDS             complement(47..517)
                     /gene="nuoE"
                     /locus_tag="P5201_12295"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014807499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoE"
                     /protein_id="HRV99368.1"
                     /translation="MAFSVEEIREKTRKITEPWRERNGGLIPILQAVQAQLGYLPQEA
                     LETISRELKIPPSEIYGVATFYAQFHLKPRGRHVVRVCRGTACHVRGSLKILEKVKNL
                     IGVGENETTEDLRFTIEPVACLGACGLAPVMMVDTQTFGRLVPDMVKGILDKFE"
     gene            709..>1639
                     /locus_tag="P5201_12300"
     CDS             709..>1639
                     /locus_tag="P5201_12300"
                     /inference="COORDINATES: protein motif:HMM:NF013183.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-binding protein"
                     /protein_id="HRV99369.1"
                     /translation="MARIEYVNIEDALGMALSHDMTQIDVKSGYKGARFKRGHIVKPE
                     DLPVLRSMGKNSISVLYLEEGDVHEDDAALRLAHRLAGEGLRMEGPEEGKVSLFAEWN
                     GLIVYDEESIHTINEDVDWIAVTTPNKVPVKKGEPAAGIRVVPLTMREEQIRKAEAAA
                     LPLTVFPFHPLKTALVTTGREIVEGRIRDAFAPKLVRKLAFYGSTLMEQVITGDEKDE
                     IARAIQSFIDKGAELIIVTGGMSVDADDRTGAAIVQVSDSVVFNGVPALPGARLMLAL
                     QDKTKIIGAPACVVHDEWTSMDILLNRLFAGLIP"
BASE COUNT          399 a          468 c          423 g          349 t
ORIGIN      
        1 atcgagactc gtgatctttt tctgctccat tcgctcttcc cccgttctac tcgaacttgt
       61 caagaatgcc cttcaccatg tcgggaacga gccttccgaa tgtctgcgta tcgaccatca
      121 tcaccggggc aagaccgcat gcgccgagac aggccacagg ctctatagta aaccggagat
      181 cctcagtggt ctcgttctcg ccaaccccga taagattctt caccttctcg agaatcttga
      241 gacttccccg aacgtggcaa gccgtccccc tacagacgcg cacaacatgt cgtccccgag
      301 gcttcaggtg aaattgggcg taaaacgtcg caacgccgta aatttcagac ggcgggatct
      361 tcagctcccg ggaaatagtt tccaacgcct cttgcggcaa atacccaagc tgcgcctgaa
      421 cagcctgtaa aatcgggata aggccgccgt tcctctctct ccagggttct gtaatctttc
      481 gtgtcttctc gcggatttct tcaacgctga aagccatgat tcccctcctt ttttaacaga
      541 acataggatt gtgcacaata tcacaatgaa aaatatcata tcataggcag gaaccaaaaa
      601 caagtaattg aaaagtgtat actttatatt gccgatacat ctccccgaca tgctataata
      661 gccgcggttt tcatcgtaaa gatgttttat aaagagcgag gggaaagaat ggccagaata
      721 gagtacgtaa acattgaaga tgcgttggga atggctcttt cgcacgacat gacccagatc
      781 gatgtcaagt ccggttacaa aggcgcccgc ttcaagagag gacacattgt caaacctgaa
      841 gatcttccgg tgctcaggag catggggaag aattccattt cggtcctcta cctggaggaa
      901 ggcgacgttc acgaagacga cgccgccctc aggcttgcac accgccttgc aggcgagggc
      961 ctccgtatgg agggtcccga ggagggaaaa gtctcgctct tcgccgaatg gaacggactg
     1021 atcgtgtacg acgaagagtc cattcacaca atcaacgagg atgtcgactg gattgccgtc
     1081 acaacgccga acaaggttcc cgtcaaaaag ggagaacccg ccgccggtat ccgcgtcgtt
     1141 ccgttgacga tgcgcgaaga gcagatccgc aaggcggaag cggctgcgct tccgctcaca
     1201 gtctttccgt tccaccctct gaaaacagcg cttgtcacca caggacgcga gatcgtcgaa
     1261 gggcgcatcc gcgacgcttt cgcgccgaaa ctggtgcgca agctggcgtt ctacggatcg
     1321 accctgatgg aacaggtcat tacaggcgac gaaaaggacg aaatcgcccg tgccattcag
     1381 tccttcatcg acaagggggc cgagctgatc atcgtcaccg gaggcatgag cgtcgacgcg
     1441 gacgaccgga ccggggcggc catagtccaa gtttccgaca gcgtggtgtt caacggcgtt
     1501 ccggcacttc cgggcgcccg cctgatgctt gcgcttcagg acaagacgaa gattatcgga
     1561 gctccggcat gcgttgtgca tgacgaatgg accagcatgg atatccttct caaccgtctc
     1621 ttcgcggggc tcatcccca
//
LOCUS       DAOTIL010000695         1639 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminobacteriaceae bacterium isolate AMR_MDS_3026
            NODE_88551_length_1639_cov_3.719066, whole genome shotgun sequence.
ACCESSION   DAOTIL010000695 DAOTIL010000000
VERSION     DAOTIL010000695.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868050
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminobacteriaceae bacterium (wastewater metagenome)
  ORGANISM  Aminobacteriaceae bacterium
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae.
REFERENCE   1  (bases 1 to 1639)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1639)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:19:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,224
            CDSs (total)                      :: 3,198
            Genes (coding)                    :: 3,184
            CDSs (with protein)               :: 3,184
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1639
                     /organism="Aminobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_88551_length_1639_cov_3.719066"
                     /isolate="AMR_MDS_3026"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:3034609"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            3..512
                     /gene="frr"
                     /locus_tag="P5201_12305"
     CDS             3..512
                     /gene="frr"
                     /locus_tag="P5201_12305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005662487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008135 - translation factor activity, RNA
                     binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome recycling factor"
                     /protein_id="HRV99370.1"
                     /translation="MEHLKNEFLAIRTGRAHPGLVSDIKVDYYGAPTPVKQIATVSIP
                     EGRQIAIAPFDRSALKLIEKAILASSLGVTPQNDGEIIRVNLPELTRDRRIELTKLVG
                     KYAEEGRIALRNLRRDSNDVLKKKEKNSEISEDDLKKYTKEVQDIIDEYIKKVDEVLK
                     AKEKEIMEE"
     gene            586..1566
                     /locus_tag="P5201_12310"
     CDS             586..1566
                     /locus_tag="P5201_12310"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009200409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008465 - glycerate dehydrogenase activity
                     [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-glycerate dehydrogenase"
                     /protein_id="HRV99371.1"
                     /translation="MSLPKVYVSRRIQDAGMRLLDGRVEYEVWEEIGPVDRRILLEKG
                     ADADGLLVTLSDRIDDELLSLCPNLKVVSNYAVGYDNIDVDAATRRGVLVTNTPDVLT
                     NATADIAFTLLLASARRMVEANEFLRSGDWVTWHPNLLLGADVSEAVLGIVGMGKIGQ
                     AVARRAKGFDMEILYVNRSPKPEIESMLGARRVSLQELLAKSDFISLHCPLSGETRGL
                     IGEKELRSMKKTAFIINTSRGPVVDQKALFKACSEGWIGGAGLDVFDKEPVPLDEPLL
                     TLKNVTTLPHLGSATIKAREGMARKAAENVLAALEGRRPADLVNPEAFKK"
BASE COUNT          420 a          403 c          464 g          352 t
ORIGIN      
        1 tcattgagca cttaaagaac gagttcttgg ctattcggac cggacgcgct catcccggtc
       61 tcgtaagcga catcaaggtg gattactacg gagcccctac gcctgtcaag caaattgcca
      121 cagtctccat tccggagggg cgtcaaattg ctatcgcccc cttcgacaga agcgctttga
      181 aactcattga aaaggcaatc ctcgcctctt cgctcggagt cactccgcag aacgacggcg
      241 aaattattcg cgtgaatctt ccggagctca caagagatcg tcgtattgaa ctgacaaaac
      301 tggtgggcaa atatgcggaa gagggacgta tcgctcttcg aaacctccgg agggattcga
      361 acgacgttct gaagaaaaag gaaaagaact cggagatcag tgaagacgac ctcaaaaagt
      421 atacaaaaga ggttcaggat attatcgacg agtacataaa gaaggtagac gaggttctca
      481 aagcgaaaga gaaagagatt atggaggaat agctttttct attcacaatc ggcaggggcg
      541 gagttttctc cgcccttttt tcagggagtt gcaggaggtg ttttcgtgtc tcttccgaaa
      601 gtatatgttt cacgtaggat acaggacgcc ggaatgcgcc tgttggatgg gcgtgtcgag
      661 tacgaagtct gggaagaaat cggccccgta gaccgccgca tactgctcga aaagggcgca
      721 gatgcggacg gtttgctggt gactctttcg gataggatcg acgatgaact tctgtcactc
      781 tgtccgaatt tgaaagtcgt cagcaattat gccgtagggt atgacaatat cgacgtcgat
      841 gcggctacac ggaggggcgt cttggtgacc aacactcccg atgtgctgac gaacgccacc
      901 gcagacattg cgtttacgct tctcttggcg agcgcacgga ggatggtgga ggcaaacgag
      961 tttctgcgtt cgggcgattg ggtgacgtgg catcccaatc ttctgctggg cgcggacgtt
     1021 tcagaagcag tgctgggtat cgtcggcatg ggaaaaatcg gccaggcggt cgcacgccgg
     1081 gcaaaaggat tcgatatgga aatcctctac gtcaaccgtt ctccgaagcc tgaaatcgag
     1141 agcatgctcg gcgccaggcg cgtctctctc caagaactgc tggctaaaag cgactttatc
     1201 tcgcttcact gcccattgag cggcgaaacg cgaggattga tcggtgaaaa agagcttcgc
     1261 tcgatgaaga agaccgcatt tattatcaac acctcgcgcg gtcctgttgt cgaccagaag
     1321 gcgcttttca aagcgtgctc cgaagggtgg attgggggag cgggactcga cgtcttcgat
     1381 aaggaaccgg ttcccttgga cgagccgctt cttacactga agaacgtaac aacgcttccg
     1441 cacctaggaa gcgcaaccat taaagccagg gaaggcatgg cgagaaaagc tgcggagaac
     1501 gtactcgcgg ctctcgaagg acgccgcccg gcagacctgg taaacccgga agcattcaaa
     1561 aaatgaagag aagcggagcg ttctgtacat tacgctccgc tttctattcc caatcggcag
     1621 aactacgcca gttcgctga
//
LOCUS       DAOTIL010000696         1639 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminobacteriaceae bacterium isolate AMR_MDS_3026
            NODE_88554_length_1639_cov_3.477273, whole genome shotgun sequence.
ACCESSION   DAOTIL010000696 DAOTIL010000000
VERSION     DAOTIL010000696.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868050
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminobacteriaceae bacterium (wastewater metagenome)
  ORGANISM  Aminobacteriaceae bacterium
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae.
REFERENCE   1  (bases 1 to 1639)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1639)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:19:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,224
            CDSs (total)                      :: 3,198
            Genes (coding)                    :: 3,184
            CDSs (with protein)               :: 3,184
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1639
                     /organism="Aminobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_88554_length_1639_cov_3.477273"
                     /isolate="AMR_MDS_3026"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:3034609"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1315)
                     /locus_tag="P5201_12315"
     CDS             complement(<1..1315)
                     /locus_tag="P5201_12315"
                     /inference="COORDINATES: protein motif:HMM:NF019581.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HRV99372.1"
                     /translation="MKKNLLRCELDTAFLHGSVVTVNEKEEVAEALGIAGNRIAYVGN
                     DRGIEPFISENTRVVDLAGRTLMPGINDTHFHPILTGLLGAHHDSGMIDTTRVRCASL
                     AGMLGMLREAVGRKRPGEWISMMGYEPELFTDERRHPTIEELDALAPENPVHCMHGGG
                     HICMYNTKALAYLDVHIPEDAGKYPPDEVEVVDGRLTGMVRGHTHFKLWGMVGYTESQ
                     QAAAAMKSHRRALEAGITSIGDMGECGPVSYHIMQKLCRDRTFKIRVNMALHSIFGKS
                     YSLNENEHWLQLGFMSGLGDERFRVGPCKFMIDGGSGGPSCATREPYSHDPDMVRERG
                     WEREETARYIERIVGAECQCTAHAIGDLAVEFMVEGYERCFRKDPEKFRRMRHRIEHC
                     SLTDQDLIDRMARMNICPSVNAGMIQAIGANYMKFYGERNAYMAAL"
BASE COUNT          278 a          510 c          499 g          352 t
ORIGIN      
        1 ggagagccgc catgtacgcg ttgcgctccc cgtagaactt catgtagttg gccccgatcg
       61 cctggatcat gcccgcgttg acggaggggc agatgttcat gcgcgccatg cggtcgatca
      121 ggtcctgatc ggtcagcgaa cagtgctcga tgcggtggcg catcctgcgg aatttttcgg
      181 ggtctttcct gaaacagcgc tcgtacccct cgaccatgaa ctcgacggcc agatcgccga
      241 tggcgtgcgc ggtgcactgg cactccgcgc cgacgatccg ctcgatatag cgcgccgtct
      301 cttcgcgctc ccagccgcgc tcgcgcacca tgtccgggtc gtgggagtag ggctcgcggg
      361 tcgcgcagga ggggccgccc gaccctccgt cgatcatgaa cttgcacggc cccacgcgga
      421 accgctcgtc gccgagtccg gacatgaagc cgagttggag ccagtgttcg ttctcattca
      481 gggagtagct cttgccgaag atgctgtgga gcgccatgtt gacgcggatt ttgaaggtcc
      541 ggtcgcggca gagcttctgc atgatgtggt acgagaccgg gccgcattcg cccatgtcgc
      601 cgatggaggt aatgcccgct tccagagccc gccggtgcga tttcatcgcc gccgcggcct
      661 gctgcgattc ggtgtagccg accatccccc acaacttgaa gtgcgtgtgt ccgcgcacca
      721 ttccggtgag ccttccgtcg acgacctcga cttcgtccgg agggtacttt cccgcatcct
      781 cggggatgtg cacgtcgagg taggcgagcg ccttggtgtt gtacatgcag atatgcccgc
      841 cgccgtgcat gcagtgcacc ggattttcgg gcgccagggc gtcgagctct tcgatagtgg
      901 ggtgccgtcg ctcgtcggta aagagctcgg gctcgtaccc catcatggag atccattctc
      961 cgggccgctt gcgtccgacg gcctcccgga gcatgccgag catcccggcc agggaagcgc
     1021 agcgcacgcg cgtcgtgtcg atcatccccg agtcgtggtg tgcgccgaga aggccggtga
     1081 ggatcggatg aaagtgcgtg tcgttgattc cgggcatgag cgtccgtccg gcaagatcga
     1141 cgacgcgggt gttttcggag atgaacggct cgatcccccg gtcgttcccg acgtaggcga
     1201 tacggttccc cgcgatcccg agcgcctcgg caacctcttc tttctcattg acggtgacga
     1261 cgcttccgtg gagaaacgcc gtatccagct cgcagcgcag taaatttttc ttcatcggac
     1321 ccactcctca tttcggaccg aatccggaga ctctcccggg aggatttcag ccgtttcgcc
     1381 gagagagact cggaataatt ccgcattatg gaatagcgtt gctctatatg gactattgaa
     1441 agcattgtag ccgtaccgcc tgttttgtca atactttctc tgcgacgcaa aacattgata
     1501 attttttaac aacttactca ccaaggcgcc gctttctttc tgtatgcgga ggcttcgcgg
     1561 aacttccgca gcgttccagc tcggggagcg aagccggttc cgggcggcat tccccgcctc
     1621 ctcgcaatac acaagcgcc
//
LOCUS       DAOTIL010000697         1638 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminobacteriaceae bacterium isolate AMR_MDS_3026
            NODE_88621_length_1638_cov_3.654454, whole genome shotgun sequence.
ACCESSION   DAOTIL010000697 DAOTIL010000000
VERSION     DAOTIL010000697.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868050
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminobacteriaceae bacterium (wastewater metagenome)
  ORGANISM  Aminobacteriaceae bacterium
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae.
REFERENCE   1  (bases 1 to 1638)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1638)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:19:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,224
            CDSs (total)                      :: 3,198
            Genes (coding)                    :: 3,184
            CDSs (with protein)               :: 3,184
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1638
                     /organism="Aminobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_88621_length_1638_cov_3.654454"
                     /isolate="AMR_MDS_3026"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:3034609"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            336..>1638
                     /locus_tag="P5201_12320"
     CDS             336..>1638
                     /locus_tag="P5201_12320"
                     /inference="COORDINATES: protein motif:HMM:NF015894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FapA family protein"
                     /protein_id="HRV99373.1"
                     /translation="MSETMNSDMQAPAAGGRSTTIRKGDVLLQLEGGGVWLACRGDSV
                     PFPADVEAALEEGGVTERDEDALRKFLDGKDRRVLIASAFVPVDAKVKVAMAKDSMSV
                     SLLVSPPSLGGAMPSPDELEEQLHRHGAVYGIDKSALAKLCAEKLFDEYVEVARGTPA
                     VHGSDAAVEVVVGTGGRRGPVEDEDGNVDLKNLGIVNLVHAGDVLATKTPATEGVEGH
                     TVRGGVLGAKNGKDKSFPAGPGTVVSEDGLSLLAAVEGHLTEVNGRLQVLPVFEVNGD
                     VDYSTGNIDFPGSVVVKGVVRDGFEVRASENITVNGTVEGAFLVAGGNITVVGGIRGV
                     SKGKVEAKGNIAADFADQATLFADGDILIKNALLHSDCRCGGKLVVSGGKKSQIAGGK
                     IQAGSEVVCATLGSEMGTKTEIAVGALPEQVERRDFLRKELH"
BASE COUNT          314 a          445 c          569 g          310 t
ORIGIN      
        1 cctccgcgat gcttaccgcg agcaggagca gcagcagcgc cgcaatacag cggaggctcc
       61 ctttccccgg tgcgctttca gggtatatgt tcattgatct ccatcccctt tttctgtgtt
      121 ttcatgcctc tattgtatag ctctttcatt cagcgtgatc tgtagttggc gcaaagattt
      181 gtaagggaaa gaaccgaagt gtaaagccgg cggtgcagtg tacccccgtt gagtgtgcgc
      241 gttcgttgcc gttttcgatg gaattggtgt atcattgata aaaatacggt gttttccgcc
      301 gtcccccggg cggcgtacag gaaagggaga tgcgtatgag cgagacgatg aacagcgata
      361 tgcaggcccc cgccgcaggc ggacggtcga cgacgatccg gaagggagac gtcctcctgc
      421 agctcgaagg agggggcgtc tggctcgcct gtcggggcga ttccgttccc tttcctgcgg
      481 acgtcgaggc cgccctggag gagggcggtg tgactgaaag agatgaagat gctctccgga
      541 agttcctcga tggaaaggat cgccgggtgc tcatcgcctc cgcgttcgtt cccgtcgacg
      601 cgaaggtgaa ggtcgccatg gcgaaggact ccatgtcggt ctcgcttctt gtttctcccc
      661 catctctcgg cggcgccatg ccttcgcccg atgagctgga ggagcagctt cacaggcacg
      721 gcgcggtgta cgggatcgac aaaagcgcgc tggcgaagct gtgcgccgaa aagctcttcg
      781 acgagtatgt cgaggtcgcc cgcggaacgc ccgcagttca tgggtcggat gcggccgtgg
      841 aggtcgtcgt tggaaccgga gggcgcaggg ggccggtcga ggacgaagat ggcaatgtgg
      901 atcttaaaaa tttgggcatc gtcaacctgg tgcatgccgg ggacgtgctt gcaacgaaga
      961 cgcctgcaac ggagggcgtg gaggggcata ccgtccgggg cggcgttctc ggagccaaga
     1021 acggcaagga caagtccttt ccggccgggc ccgggacggt cgtctccgag gacgggcttt
     1081 cgctgctcgc cgccgtcgag gggcatctta cggaggtcaa cggacggctc caggtgcttc
     1141 cggtttttga agtgaacggc gacgtcgact acagcacggg gaacatcgac tttcccggat
     1201 cggtcgtggt gaagggggtc gtccgggacg gtttcgaagt tcgcgcttcg gagaacatca
     1261 cggtgaacgg caccgtcgag ggcgcgtttc tggtcgccgg aggcaatatc accgtcgtcg
     1321 gcggcatccg cggcgtcagc aagggaaagg tcgaggcgaa gggcaacatc gcggcggact
     1381 tcgccgacca ggcgacgctc ttcgcggacg gcgacatcct gatcaagaac gcgctgcttc
     1441 acagcgactg ccggtgcgga ggaaagcttg tcgtctcggg gggaaaaaag tcgcagatcg
     1501 ccggaggaaa gatacaggcc ggaagcgagg tcgtctgcgc cacgctcggc agcgaaatgg
     1561 gcacgaagac ggagatcgcg gtcggcgcgc tgccggaaca ggtggaacgg cgggatttcc
     1621 tgaggaagga actgcacc
//
LOCUS       DAOTIL010000698         1638 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminobacteriaceae bacterium isolate AMR_MDS_3026
            NODE_88626_length_1638_cov_3.425774, whole genome shotgun sequence.
ACCESSION   DAOTIL010000698 DAOTIL010000000
VERSION     DAOTIL010000698.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868050
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminobacteriaceae bacterium (wastewater metagenome)
  ORGANISM  Aminobacteriaceae bacterium
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae.
REFERENCE   1  (bases 1 to 1638)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1638)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:19:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,224
            CDSs (total)                      :: 3,198
            Genes (coding)                    :: 3,184
            CDSs (with protein)               :: 3,184
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1638
                     /organism="Aminobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_88626_length_1638_cov_3.425774"
                     /isolate="AMR_MDS_3026"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:3034609"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..371)
                     /locus_tag="P5201_12325"
     CDS             complement(<1..371)
                     /locus_tag="P5201_12325"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRV99374.1"
                     /translation="MVCRALSHQNHPLKIRRVRSPDTDPWKNLAREEFLMGDCADDEA
                     IFYLWQNAHTVVIGRNQNAWSECRLDLLESEGGRLARRSSGGGAVYHDLGNLNYSFIM
                     PRPLYSMERQLGVVLAALRSLG"
     regulatory      complement(466..573)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00504"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="glycine riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="glycine"
                     /db_xref="RFAM:RF00504"
     gene            complement(765..1316)
                     /locus_tag="P5201_12330"
     CDS             complement(765..1316)
                     /locus_tag="P5201_12330"
                     /inference="COORDINATES: protein motif:HMM:NF013629.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fur family transcriptional regulator"
                     /protein_id="HRV99375.1"
                     /translation="MTNNTRTDETVQAFLEKLKDRGLRLTAQRETLFRVIAEDLGNPT
                     SVQNIWDRTREVDPSIGIATIYRTLNLLGEMGVVNIIYLSDGEFRLDVPLQKIHLTAF
                     CRHCGALFPLPAEDEKQHTLEKWLEGTGLELLPQSVAVAGLCTECREALKSPDALPAR
                     RGPLGQQGPCLRRRCRRMGRVQE"
BASE COUNT          302 a          456 c          526 g          354 t
ORIGIN      
        1 ccgagcgagc gcagtgcggc gagaacgacg ccgagctgcc gttccatgct gtagagaggt
       61 cggggcatga tgaaggagta gttgaggttg ccgagatcgt ggtagaccgc gccgccgccg
      121 gaggagcgcc gggcgagccg tcctccctcg gattcgagca ggtcgaggcg gcattcgctc
      181 cacgcgttct ggttgcgccc gatgacgacg gtatgcgcat tctgccagag gtagaagatc
      241 gcctcgtcgt cggcgcagtc gcccatgagg aactcctctc gggccaggtt tttccacgga
      301 tccgtgtccg gcgagcggac acgacggatt ttcagggggt ggttttggtg cgagagcgcg
      361 cgacagacca tgaaatcgcc tccttataca ggtaagctat tctctgtcgc atagtctcga
      421 agagggacga cgtggtcgtt gcgagaacta ttcttttcag agtaccgtcc ggccgcaatc
      481 ctttcgcctg agagtttcag ccgttctttc gaaacggcct tgccccttcg gcgtctcccc
      541 tcattgcgga ggagactctc ttgcggcgtt catacggaaa gtacattcat tgtagcgaat
      601 gaaccgaaag tgtcaagcgg cgcggcggaa tagtgcgacg gaattttatt ataataaatt
      661 tgttcttgtg aacaaagagt gcgccgatga agactgctcc ggttgcacgc gcagaagggg
      721 gtcgcgcccg cgctgctttc cccggcgtag tattcggcgg tgactcactc ctgaacacgc
      781 cccattcttc ggcagcggcg gcggaggcag gggccctgtt gtcccaaggg gcctctgcgg
      841 gcggggagcg cgtcggggct tttcagcgcc tccctgcatt ccgtgcagag cccagccacg
      901 gctacggact gcggaagcag ttcgagtcct gtcccctcca gccatttctc cagagtgtgc
      961 tgtttctcat cttcggcggg gagggggaag agcgcgccgc aatgcctgca gaacgccgtg
     1021 agatggatct tctgcagcgg gacgtcgagc cggaattcgc cgtcgctcag gtagatgatg
     1081 ttcacaacgc ccatctcccc cagaaggttg agcgtccggt agatagtcgc gatgccgatc
     1141 gaggggtcca cctcgcgcgt cctgtcccag atgttctgaa ccgacgtggg gttgcccaaa
     1201 tcctcggcga tgacccggaa gagcgtttca cgctgtgcag tgaggcgaag cccccggtcc
     1261 ttcagctttt ccaggaaagc ctgtactgtc tcatccgtgc gcgtattgtt cgtcatggtg
     1321 tgttccctcc ctggttttga ggaaaattat acccctccgg cggtaccggc acgtcccggc
     1381 gttttctccg cctgccgacc cgcgagtatg catcttcccc ttgacataat gccacaggat
     1441 agcgtattat gcaaatgata attattatca ttaaaggagg agatcgagat gccgggaaga
     1501 aacggaacgg gacccatggg acaggggtcg atgaccggat gggggcgcgg cccttgcggg
     1561 cgcggcgtcg cagcaggcgg acgatttttt agagggcgcg gctgctttgg ttggggcatg
     1621 cgtatgggaa tgggtatg
//
LOCUS       DAOTIL010000699         1637 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminobacteriaceae bacterium isolate AMR_MDS_3026
            NODE_88745_length_1637_cov_1.989254, whole genome shotgun sequence.
ACCESSION   DAOTIL010000699 DAOTIL010000000
VERSION     DAOTIL010000699.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868050
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminobacteriaceae bacterium (wastewater metagenome)
  ORGANISM  Aminobacteriaceae bacterium
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae.
REFERENCE   1  (bases 1 to 1637)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1637)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:19:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,224
            CDSs (total)                      :: 3,198
            Genes (coding)                    :: 3,184
            CDSs (with protein)               :: 3,184
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1637
                     /organism="Aminobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_88745_length_1637_cov_1.989254"
                     /isolate="AMR_MDS_3026"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:3034609"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..490)
                     /locus_tag="P5201_12335"
     CDS             complement(<1..490)
                     /locus_tag="P5201_12335"
                     /inference="COORDINATES: protein motif:HMM:NF012760.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016742 - hydroxymethyl-, formyl- and
                     related transferase activity [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formyltransferase family protein"
                     /protein_id="HRV99376.1"
                     /translation="MIRRIILFGDTHGLPMLLHALPCEMVCGIVAAEIRPQYHEELQK
                     LAEEKGIPFAIQPLSKSPDYPNFVQWVRELSPQLLWVNSYSMKLREEILRIPEAGALN
                     IHGALLPYYRGCNPTQWAILRDESATGVTLHEVTAGIDEGPIIARKEVPLLFEDTWKD
                     VQD"
     gene            complement(483..1220)
                     /locus_tag="P5201_12340"
     CDS             complement(483..1220)
                     /locus_tag="P5201_12340"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRV99377.1"
                     /translation="MNNWTVFTESAYSNLLSLAKEQYEFINYTSELNAENIVLWRHDV
                     DFSVHRALRLAEVESEQGVASTFFFPLSCIFYNIFEHTVLERIRRIVKLGHTIGLHFD
                     PSLYEEKCKEAVLEKMAWEKTVLENLFETGIHAVSFHNPEYDDWLSMDAHTLCGMVNT
                     YSRYFKENFGYCSDSNGYWRFESIESVLRSKKYSRLQVLTHPAWWTAEPMPPRKRIQR
                     CVEGRAKQCMEQYDGILAKSGRMNIDD"
     gene            complement(1217..>1637)
                     /locus_tag="P5201_12345"
     CDS             complement(1217..>1637)
                     /locus_tag="P5201_12345"
                     /inference="COORDINATES: protein motif:HMM:NF014908.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRV99378.1"
                     /translation="ALDLLEANPEYVLERQSKNPADALRCYPRKPEDGRIDWNRSAEE
                     ILRLINASTKPYAGAFTYLKGERVRIWSARIGALPCPSCFVPGQVLWKNEDGSVAVGT
                     GNSGVLLLDEVQKDAGEPCKAAEMVRSLRERFGRNGQ"
BASE COUNT          364 a          475 c          409 g          389 t
ORIGIN      
        1 ggtcctgaac atccttccag gtatcttcaa agaggagcgg aacctctttc cgcgctatga
       61 tcggcccctc gtcgatgccc gcagtaacct cgtgcagcgt tacgccggtc gcgctctcgt
      121 ccctcagaat tgcccactgc gtcggattgc acccgcgata ataggggaga agagccccgt
      181 gaatgttcaa tgcgcctgct tcgggaatcc gcagaatctc ctcgcggagc ttcatgctgt
      241 acgagttcac ccagagaagc tgcggcgaca actcccgaac ccattggaca aaattggggt
      301 agtctggcga cttggacaaa ggctgtatgg caaacggaat gcccttctct tcggcaagtt
      361 tctggagttc ctcgtgatat tgcggtcgaa tttctgcagc tacaataccg cagaccatct
      421 cacagggcaa agcgtggagg agcatcggaa gaccgtgcgt atccccaaag aggatgatgc
      481 gcctaatcat caatgttcat cctccccgac ttagcgagta ttccgtcgta ttgctccata
      541 cactgttttg ccctaccctc cacgcaccgc tgaatccgct ttctcggggg catgggctcc
      601 gcagtccacc atgctgggtg tgtcagcacc tgaaggcggg aatacttttt gctccgaaga
      661 accgattcta tgctctcaaa ccgccagtag ccgttggagt cggagcaata gccgaagttt
      721 tccttaaaat atcgcgaata ggtgtttacc atgccacaga gagtgtgggc atccatcgag
      781 agccagtcgt cgtactccgg attgtgaaac gagactgcgt gaatcccggt ctcaaacaga
      841 ttttcgagaa ctgtcttctc ccacgccatt ttctccaaaa cggcctcctt acacttctct
      901 tcgtatagag aaggatcaaa gtggaggccg attgtgtgcc ccagctttac gattcgcctg
      961 atgcgctcaa gaaccgtatg ttcgaaaatg ttgtaaaaaa tacagcttag cgggaaaaag
     1021 aaggtgcttg cgacaccctg ctcgctttcg acctctgcca aacgaagtgc tctgtgtacg
     1081 gagaaatcga catcgtgtct ccacagaacg atattttccg cattcagttc actcgtgtag
     1141 ttgataaact catactgctc cttcgcaagc gaaaggaggt tcgagtatgc gctttcagtg
     1201 aagaccgtcc agttgttcat tgtccgttcc tcccgaaccg ctccctcagc gaccgtacca
     1261 tctccgccgc cttgcacggc tcgcccgcat ccttctgcac ctcatccagc agcagcactc
     1321 ccgaattccc cgttcccaca gccacggagc cgtcctcgtt cttccacaga acctgtccgg
     1381 ggacgaagca gctcgggcag gggagggcgc ctatgcgggc gctccagata cgtacacgtt
     1441 cccccttaag gtatgtgaaa gctccggcat agggttttgt gcttgcgttg atgaggcgaa
     1501 gaatctcctc ggcacttctg ttccaatcaa tacgtccatc ctcgggcttg cgtggataac
     1561 agcgaagggc atctgcggga tttttggatt ggcgttccag tacatactca gggtttgcct
     1621 ccaaaagatc aagagcc
//
LOCUS       DAOTIL010000700         1635 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminobacteriaceae bacterium isolate AMR_MDS_3026
            NODE_88893_length_1635_cov_2.657595, whole genome shotgun sequence.
ACCESSION   DAOTIL010000700 DAOTIL010000000
VERSION     DAOTIL010000700.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868050
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminobacteriaceae bacterium (wastewater metagenome)
  ORGANISM  Aminobacteriaceae bacterium
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae.
REFERENCE   1  (bases 1 to 1635)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1635)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:19:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,224
            CDSs (total)                      :: 3,198
            Genes (coding)                    :: 3,184
            CDSs (with protein)               :: 3,184
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1635
                     /organism="Aminobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_88893_length_1635_cov_2.657595"
                     /isolate="AMR_MDS_3026"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:3034609"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..397
                     /locus_tag="P5201_12350"
     CDS             <1..397
                     /locus_tag="P5201_12350"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HRV99379.1"
                     /translation="FPDLDGFKEINDTYGHETGDVLLVSIARRLDEAVREQDTVARLG
                     GDEFILLFEGLGGVEEAHSLAKRVDALFAEPFVAGGRPFLCSASAGLAVHRPGDGATA
                     QEMLAIADMRMYEAKAQRKTSKALSGSRA"
     gene            614..>1635
                     /locus_tag="P5201_12355"
     CDS             614..>1635
                     /locus_tag="P5201_12355"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRV99380.1"
                     /translation="MCLLFAILGGILGYWEMRRASRHVQEELLRTARLLSESVNPRRL
                     QALSGSASDLYLPDYSRLNEQLLSVLPVLPRGSSISILGRREDGALVYLMDVRAAASA
                     LPGDEFRVRSSDFQYPFDERRPLLLGPFAHDDGSFFAALVPLVEPVTRRVIAVFLLET
                     DARNWRREPYRAALVPWGATILLLMVLFLGYRFSPRLRDSRVFVYAEVLWVASMSLLL
                     AGSCAWLVGAYEFRGRTRAFSMLSDFGSSALIQSLQELRDCSIEGLGRFFESSQLVDA
                     EEFAGFARHLIAIPHVDEVLWAPAVPSLAAESGEPSEAGLLSSGDLAEGPSMPSSSSY
                     ALSFPVL"
BASE COUNT          252 a          505 c          519 g          359 t
ORIGIN      
        1 ctttcccgac ctcgacggct tcaaggagat caacgacacg tacggacacg agaccggcga
       61 cgtcctgctc gtttcgatcg cccgcagact cgacgaggcg gtgcgggagc aggatacggt
      121 cgccagactg gggggcgacg agttcattct cctcttcgag gggcttggcg gcgtcgagga
      181 ggcgcattcg ctcgccaaga gggtggatgc gctgttcgcc gagccgtttg tcgccggagg
      241 tcgtccgttc ctctgcagcg ccagcgcggg gctggccgtt caccgtcccg gggacggggc
      301 gacggcgcag gagatgcttg cgatcgccga tatgcggatg tacgaagcaa aggcgcagcg
      361 aaagacctcg aaggccctct cgggcagccg ggcgtgagca atgtgcctga agagtgcgta
      421 ttgccggaga agattgttct gtggtatgat atttttgttt ctgcggacga aaaagggggc
      481 cttcccgctg catttttgat ttgcggcgca agcgggaacc ggcgtaaagc accctgcgaa
      541 cagtggagag aggatcccgg ccatggagaa agaggttcgg tctcgcggag catttgagcg
      601 gggagtcgtc gtcgtgtgtc tccttttcgc aatcctcgga ggcattctcg ggtattggga
      661 gatgcggcgc gcttcgcggc acgttcagga ggagctgctc cggacagccc gcctcctttc
      721 ggagagcgtg aacccgcggc gtctccaagc cctttccggc agcgcttccg acctgtacct
      781 tccggactac tcccgcctga acgagcagct cctctcggtt cttcccgtgc tgccccgcgg
      841 ctcttccata tctattctcg ggaggaggga ggacggcgcc ctcgtctacc tgatggacgt
      901 gcgcgccgcc gcatccgcgc tccccggcga tgaattccgt gtccggtcct ccgacttcca
      961 ataccctttc gacgaaagac ggccgcttct gctcggtcct ttcgctcacg acgacggctc
     1021 tttttttgcg gcgctggtcc ctttggtgga accggtgacg cgccgtgtca tcgccgtatt
     1081 ccttcttgaa accgacgccc ggaactggcg gcgcgagccg tacagggcgg cgcttgttcc
     1141 gtggggagcg acgatactcc tcctcatggt tcttttttta ggctaccgct tctccccacg
     1201 actccgcgac tcgagggttt tcgtgtacgc ggaggtgctc tgggttgcct cgatgagtct
     1261 tctcctcgcc ggatcgtgcg catggctcgt gggcgcgtac gaattccgcg ggcgtacgag
     1321 ggcgttctcc atgctctccg acttcggctc cagcgccttg atacagtcgc tccaggagct
     1381 gcgggattgc agcatcgaag gattgggacg ttttttcgag agcagccagc ttgtcgacgc
     1441 cgaagaattt gcgggcttcg ccagacatct gatcgccata ccccatgttg acgaagtgct
     1501 gtgggcgccc gcagtgccct ctctggccgc cgaaagcggc gaaccgtccg aagccgggct
     1561 tctctcttcc ggggatttgg cggaaggacc ttccatgcct tcttcatcct cctacgcgct
     1621 ctccttcccg gtgct
//
LOCUS       DAOTIL010000701         1635 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminobacteriaceae bacterium isolate AMR_MDS_3026
            NODE_88909_length_1635_cov_2.203797, whole genome shotgun sequence.
ACCESSION   DAOTIL010000701 DAOTIL010000000
VERSION     DAOTIL010000701.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868050
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminobacteriaceae bacterium (wastewater metagenome)
  ORGANISM  Aminobacteriaceae bacterium
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae.
REFERENCE   1  (bases 1 to 1635)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1635)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:19:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,224
            CDSs (total)                      :: 3,198
            Genes (coding)                    :: 3,184
            CDSs (with protein)               :: 3,184
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1635
                     /organism="Aminobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_88909_length_1635_cov_2.203797"
                     /isolate="AMR_MDS_3026"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:3034609"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1605)
                     /locus_tag="P5201_12360"
     CDS             complement(<1..1605)
                     /locus_tag="P5201_12360"
                     /inference="COORDINATES: protein motif:HMM:NF016859.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta'"
                     /protein_id="HRV99381.1"
                     /translation="MKCYGRDLGRGYSVKLGTAVGIIAAQSIGEPGTQLTMRTFHTGG
                     VRLTGEDITQGLPRIEQLFEVRRPKKVAFLAGVDGRVTEVRVMEGKRKVIITAENPTG
                     DGEEKVTYNIPINQNLLVNEGDSVLKSDKLTEGNFDPQQLLEVEGIEAVQRALVDEIQ
                     EVYRSQGVSISNKHIEVILRKVAPVNRVKINEEGDTSFVAGDLVWTDDIDAETAEIGR
                     ENERNIDEAVRIFTGIPLKDVSAQKMNEAVLQHLGKPLDEEAIRTILRPGMLISSAIL
                     TDTEGKQFNLVVGEAAFRKQLEGLELIEAFQSADGRRIEAGTRLTLGQLAIVTSNDPT
                     PLLVRDEALLEKLVDTAYIAEDIVADGESVAKADRVFTLEDAALCRERGVKSVMVWHS
                     VERVNLLDILEESLKEKWGKSLTRAIDIEGNAIADLPQLVDERVIKGILEGRIGAVEI
                     EGAVFSRDHFMKELLSARVYGKILLEPVVDARGNVLAEAAQEVNQTVIERLSGSAGPT
                     DASGNGVKELVVRPMGGHKKEEKKIIQ"
BASE COUNT          268 a          532 c          459 g          376 t
ORIGIN      
        1 ctggatgatc ttcttttctt ctttcttgtg ccctcccatc gggcggacaa caagttcctt
       61 gacaccgttt ccggaggcat ccgtcgggcc ggcgctgcct gagagtcttt cgatgaccgt
      121 ctgattgacc tcctgcgccg cctccgcgag cacatttcct cgggcgtcga cgacaggctc
      181 aaggagtatc tttccgtaga ctctggcgga gaggagttct ttcatgaagt gatcccgtga
      241 gaagacagcg ccctctattt caacggcccc gatacgccct tcgagaatcc ccttgattac
      301 tctctcgtcg acaagctgcg gaagatcggc gatagcgttc ccttcaatgt cgatggcgcg
      361 cgtgagcgac ttgccccact tctccttcag gctttcctca aggatatcaa gaagattgac
      421 ccgttccacc gagtgccata ccatcacgct cttcacgccg cgctctctgc aaagagcggc
      481 gtcctccagc gtaaagactc tgtctgcttt cgccacggac tctccatccg cgacaatgtc
      541 ctcggcgatg tatgcggtgt cgaccagctt ttcaagcagc gcctcgtccc gcaccagcag
      601 gggcgtcgga tcattggagg tgacgatggc gagctgaccg agcgtcagcc gagttcctgc
      661 ctctatcctt ctcccgtccg cggactggaa ggcttcgatg agttcgagcc cctcaagctg
      721 cttccggaag gcggcttcgc cgaccacgag gttgaactgc tttccttccg tatccgtcaa
      781 tatggcggag gagatcagca ttccggggcg gagtatcgtc cggatcgcct cttcgtcgag
      841 cggcttgcca aggtgctgca gaacggcttc attcatcttc tgggcgctca cgtccttcaa
      901 cggtatcccc gtgaagatgc gcaccgcctc gtcgatgttc cgctcgttct cgcggccgat
      961 ctccgccgtc tcggcgtcga tgtcgtccgt ccagacaagg tctcccgcga cgaaggatgt
     1021 gtcgccctcc tcgttgatct tcacgcggtt caccggggcc accttccgca gaatgacctc
     1081 gatgtgcttg ttgctgatcg agacgccctg cgagcggtag acttcctgta tttcatccac
     1141 gagcgcgcgc tgcaccgcct cgattccctc gacctcgaga agctgctgcg ggtcgaagtt
     1201 cccctcggtg agcttgtcgc tcttgagcac gctgtcccct tcgttcacca ggagattctg
     1261 gttgatgggg atgttgtacg tgactttttc ctcgccgtcc cccgtcgggt tctcggcggt
     1321 gatgatgacc ttccgcttcc cctccatgac gcggacttcg gtcacgcgcc cgtccacgcc
     1381 cgcgaggaag gcgaccttct tcggacggcg gacctcgaag agctgttcga tgcgcggaag
     1441 accctgcgtg atgtcctctc ccgtgagacg gacgccgccg gtatggaagg tccgcatcgt
     1501 gagctgcgtt cccggttcgc cgatggactg ggcggcgatg atgccaacag cggtgccaag
     1561 cttcaccgag tagccgcggc caaggtcgcg gccgtagcac ttcacgcaga taccgcgagg
     1621 aagttcgcag cacat
//
LOCUS       DAOTIL010000702         1628 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminobacteriaceae bacterium isolate AMR_MDS_3026
            NODE_89396_length_1628_cov_4.401780, whole genome shotgun sequence.
ACCESSION   DAOTIL010000702 DAOTIL010000000
VERSION     DAOTIL010000702.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868050
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminobacteriaceae bacterium (wastewater metagenome)
  ORGANISM  Aminobacteriaceae bacterium
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae.
REFERENCE   1  (bases 1 to 1628)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1628)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:19:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,224
            CDSs (total)                      :: 3,198
            Genes (coding)                    :: 3,184
            CDSs (with protein)               :: 3,184
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1628
                     /organism="Aminobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_89396_length_1628_cov_4.401780"
                     /isolate="AMR_MDS_3026"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:3034609"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>1628
                     /locus_tag="P5201_12365"
     CDS             <1..>1628
                     /locus_tag="P5201_12365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013048021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta'"
                     /protein_id="HRV99382.1"
                     /translation="IVIGPHLKIYQCGLPKKMALELFKPFVIRQLVERNLAPNVKSAK
                     RIIERGREEIWGILEEIVKDHPVMLNRAPTLHRLGIQAFEPVLMEGKAIRLHPMVCTA
                     FNADFDGDQMAVHVPLSIEAQTEARLLMLSANNLLSPASGRPVMTPTQDIVLGIYYLT
                     DMRDGLPGDGMYFSDIDDALAAFHHGVTKVNSRIFLRRRDEWNHALGNVEGKWIETSP
                     GRVIFNSRLPEPLRFINRQLGKKDLGQLLDRAFDLVDRVSMVTMLDNIKSLGYHWATV
                     SGISFGVQSIIIPPEKRELTAKAMEQDEELKSEYNMGILTPDEYLFQKENLWSETSRA
                     IADSIVDHMDRTNPIRMMSDSGARGSRSQMGQMAGIRGLMSDPSGRIIDYPITANFRE
                     GMNMLEYFISTHGARKGLADTALRTAKSGYLTRRLVDVAQDLIITQHDCGTNQGVLIR
                     PLTQDGKVMIPLSERIYGRTSLHDIEHPETGGMIVASGECITDEKARIIEKSGIEEVW
                     VRSPLSCALRHGICQVCYGMDLATRKEVPMGEAV"
BASE COUNT          346 a          530 c          497 g          255 t
ORIGIN      
        1 tcatcgtcat cggcccgcac ctcaagatct accagtgcgg cctgccgaag aagatggcgc
       61 tcgagctctt caagccgttc gtcatcaggc agctcgtcga gcgcaacctc gcccccaacg
      121 tcaagagcgc gaagcgtatc atcgaacggg gacgggagga gatctgggga atcctcgagg
      181 agatcgtcaa ggaccacccg gtcatgctca accgcgcgcc gacgctccac aggctcggca
      241 tccaggcgtt cgagcccgtg ctcatggaag gaaaggcgat ccgccttcac cccatggtct
      301 gcacggcctt caacgccgac ttcgacggcg accagatggc cgtccacgtg ccgctctcca
      361 tcgaggcgca gaccgaggcg agactcctca tgctctcggc caacaacctg ctgtcgcccg
      421 cgagcggccg ccccgtcatg acgccgacgc aggacatcgt gctcggcatc tactacctga
      481 cggatatgcg cgacgggctt cccggcgacg gcatgtactt cagcgacatc gacgacgcgc
      541 tcgccgcctt ccatcacggc gtcaccaaag tgaacagccg tatcttcctg cggcggcgcg
      601 acgagtggaa ccacgccctt ggaaatgtgg agggcaaatg gatcgaaacc tctccggggc
      661 gcgtcatctt caacagccgg ctccccgagc cgctgcgctt catcaacagg cagctcggca
      721 agaaggacct cggacagctg ctcgacaggg cgttcgacct cgtcgacaga gtttccatgg
      781 tgacgatgct cgacaacatc aagtcgctgg ggtaccactg ggcgacggtc agcggcatca
      841 gcttcggcgt gcagtcgatc atcatcccgc cggaaaagcg cgagctcacg gcgaaggcca
      901 tggagcagga cgaagagctg aagtcggagt acaacatggg tattctcacc ccggacgagt
      961 acctcttcca gaaggagaac ctgtggtcgg agacgtcgcg cgccatagcc gacagtatcg
     1021 tcgaccacat ggacaggacg aatcccatcc gcatgatgag cgactccgga gcgcggggaa
     1081 gccgcagcca gatggggcag atggccggaa tccggggcct gatgtccgac ccctcgggcc
     1141 gcatcatcga ctacccgatc accgcgaact tccgcgaggg gatgaacatg ctcgagtact
     1201 tcatctcgac gcacggcgcc cgaaagggac ttgccgacac cgccctcaga accgccaagt
     1261 cggggtatct tacccgccgc ctggtggacg tggcgcagga cctgatcatc acgcagcacg
     1321 actgcggaac gaaccagggt gtgctcatcc gccccctgac gcaggacgga aaggtcatga
     1381 ttccgctttc cgagcgtatc tacggcagaa cgtcgctgca tgatatcgaa catccggaga
     1441 ccggcgggat gatcgtcgcg tcgggcgagt gcatcacgga cgagaaggcg cggatcatag
     1501 agaaatccgg cattgaagag gtctgggtcc gcagcccgct gagctgcgcg cttcgccacg
     1561 gcatctgcca ggtctgctac ggcatggacc tggccaccag aaaggaagtc ccgatgggag
     1621 aggccgtc
//
LOCUS       DAOTIL010000703         1622 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminobacteriaceae bacterium isolate AMR_MDS_3026
            NODE_89837_length_1622_cov_8.450542, whole genome shotgun sequence.
ACCESSION   DAOTIL010000703 DAOTIL010000000
VERSION     DAOTIL010000703.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868050
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminobacteriaceae bacterium (wastewater metagenome)
  ORGANISM  Aminobacteriaceae bacterium
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae.
REFERENCE   1  (bases 1 to 1622)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1622)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:19:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,224
            CDSs (total)                      :: 3,198
            Genes (coding)                    :: 3,184
            CDSs (with protein)               :: 3,184
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1622
                     /organism="Aminobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_89837_length_1622_cov_8.450542"
                     /isolate="AMR_MDS_3026"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:3034609"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..621
                     /locus_tag="P5201_12370"
     CDS             <1..621
                     /locus_tag="P5201_12370"
                     /inference="COORDINATES: protein motif:HMM:TIGR02384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelB/DinJ family
                     antitoxin"
                     /protein_id="HRV99383.1"
                     /translation="RKMEENCEALEGADALLLLSEWALFRRLDFPRMKDLLKTPLIFD
                     GRNPYSPPAMKSWGSSACASDDVEVLPENAERESCFSGRTWGAWRSFCEACRNVTLAV
                     KAFLNYNVIQTKRGGDFMAANSLVQARIDPEVKKEASVVLASMGLSVSDAVRLMLVRV
                     AAEKALPFDPLIPNETTLAAMREARAGGLPSVGSVGELMDALNAED"
     gene            608..886
                     /locus_tag="P5201_12375"
     CDS             608..886
                     /locus_tag="P5201_12375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011114071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system YafQ family
                     toxin"
                     /protein_id="HRV99384.1"
                     /translation="MRKIEWTSQFKRDFKREKKSHHQSILESALMTVVQALAEDRPLE
                     AKCCDHALGGRWGGCRDCHIRPDLLLIYEKEEGVLRLIRLGSHAEIGF"
     gene            1133..>1622
                     /locus_tag="P5201_12380"
     CDS             1133..>1622
                     /locus_tag="P5201_12380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005659791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose 6-dehydrogenase"
                     /protein_id="HRV99385.1"
                     /translation="MRICVVGTGYVGLVTGACFAEVGNDVCCVDVDERKIAMLQEGRI
                     PIYEPGLEEIVKRNASQGRLRFTTSLKEGMERALFVFIAVGTPPDGTGAADLSFVERV
                     AREIGENMESYKVVVTKSTVPVGTTLRVKEIVGEQVALRGRTDLEFDVAFCPEFLKEG
                     TAV"
BASE COUNT          314 a          371 c          605 g          332 t
ORIGIN      
        1 cggaaaatgg aggagaactg cgaagcgctg gagggcgcgg atgcgctgct gctgctgagc
       61 gagtgggcgc tcttccggag gctggacttc ccgaggatga aggatctgtt gaagactccg
      121 ctgatcttcg acgggaggaa cccgtattct ccccccgcga tgaagtcgtg gggttcgagt
      181 gcgtgtgcat cggatgatgt tgaggtgtta cctgagaacg cggagcgaga aagctgcttt
      241 tcggggcgta cttggggcgc gtggcgctcg ttttgcgaag cttgtcgaaa cgtgactctt
      301 gccgtaaaag cttttttgaa ttacaatgta attcaaacga aacgaggagg ggatttcatg
      361 gctgcgaaca gtcttgtaca ggcgaggata gatccggaag tcaagaagga agcctccgtc
      421 gtgctggcct cgatggggct ttccgtttcc gatgcggtac gtctgatgct cgtgagagtc
      481 gcggcggaaa aagcgttgcc gttcgatccg ctgattccta acgagaccac tttggccgcg
      541 atgcgggaag caagggcggg ggggcttccg agtgtcggga gcgtcggcga gcttatggat
      601 gcgctgaatg cggaagattg agtggacaag ccagtttaaa cgtgacttca agagagaaaa
      661 gaagagtcat caccagagta ttctcgagag cgcgcttatg acggttgtcc aagcgcttgc
      721 cgaagaccgt ccgctggagg cgaaatgctg tgatcacgct ttgggcgggc gatggggcgg
      781 gtgcagagac tgtcatatca gacccgatct tctgctgatc tacgaaaaag aagagggtgt
      841 tctccggctg attcgtctgg gttcgcacgc ggaaatcgga ttctgagatc ttcgccgctc
      901 ggaatgcttc aggggggctg agcgtcgccg acgccttgaa gttcgccgat ctcgccgacg
      961 agatacggcg ctgacttcag gagccggcgg ggctttcaga aaaacgaacc tgcggatgcg
     1021 agcagtgtcg ggtataatgc aaggcgtttc tctgtgaaaa ctatagcgat ccggggcgcg
     1081 ttgcgcggct tcggttcgct ggaatgcatt cttgttcggg agttgatcgt gtatgcgcat
     1141 ttgcgtggtg ggaaccgggt acgtcgggct tgtgacgggg gcgtgcttcg cggaggtggg
     1201 gaacgacgtc tgctgcgtgg acgtggacga gcggaagatc gcgatgctgc aggaggggcg
     1261 tattcccatc tacgagccgg ggctggagga gatcgtgaag cggaacgcgt cgcaggggcg
     1321 gctgcgcttc acgacgagcc tgaaggaggg gatggagcgc gcgctcttcg tcttcatcgc
     1381 ggtggggacg ccgcccgacg gcacgggagc ggcggatctg tcgttcgtgg agcgggtggc
     1441 gcgggagatc ggggagaaca tggagagcta caaggtcgtg gtgacgaagt cgaccgtgcc
     1501 ggtggggacg acgctgcggg tgaaggagat cgtcggcgag caggtggcgc tgcgcgggcg
     1561 gacggacctg gagttcgacg tggccttctg tccggagttc ctgaaagagg ggacggcggt
     1621 gg
//
LOCUS       DAOTIL010000704         1622 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminobacteriaceae bacterium isolate AMR_MDS_3026
            NODE_89912_length_1622_cov_2.327377, whole genome shotgun sequence.
ACCESSION   DAOTIL010000704 DAOTIL010000000
VERSION     DAOTIL010000704.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868050
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminobacteriaceae bacterium (wastewater metagenome)
  ORGANISM  Aminobacteriaceae bacterium
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae.
REFERENCE   1  (bases 1 to 1622)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1622)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:19:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,224
            CDSs (total)                      :: 3,198
            Genes (coding)                    :: 3,184
            CDSs (with protein)               :: 3,184
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1622
                     /organism="Aminobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_89912_length_1622_cov_2.327377"
                     /isolate="AMR_MDS_3026"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:3034609"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(15..>1622)
                     /locus_tag="P5201_12385"
     CDS             complement(15..>1622)
                     /locus_tag="P5201_12385"
                     /inference="COORDINATES: protein motif:HMM:NF012311.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family serine peptidase"
                     /protein_id="HRV99386.1"
                     /translation="LLDGYAEGEALAVVQIPVSTASNVSSAESIAASVGARAVRTYGA
                     IAAHTGKHIVHIRSEHKTTEELIAALQALPEVIGAWPNYESRATRTPNDPKYGKLWGM
                     NYIGAPEAWETATGNAGIYVAVIDSGIDYNHEDLAANIGRDLDGNIGFDAVNDDADPL
                     DDYWHGTHVAGIIGATGNNALGVSGVNWRVSLLGVKVLNAEGLGYDSWIIAGLNYLLE
                     QKERGLNIRVANMSLTGWREPILDQEKNPYALACKALSDAGIILVVAAGNEKQNIDNP
                     EEYYDWSQLRWVDLRGQRPYPASFTFDHMITVGSMAGDREPSSFTNYSPNFVHLAAPG
                     TDILSTFPGNEYTTESGTSMASPHVAGTAALLAARYPRKSAAEIKARIIGNTVPNYHW
                     EGRVVFGGFLHAATALEAPDPRVPVTEIAIFPDSATLSGRASIPVAATALPLHAENKD
                     LVWFSDNPTVAVVEKTDTGATITSLSDGRATIKAHALGGVVTNAVSVTVTGAGPAVLG
                     GGGGCAVATSFMWTSVFLFIPLILLRR"
BASE COUNT          322 a          474 c          456 g          370 t
ORIGIN      
        1 gttcgtcata gtcatcatct ccttagcagt atgagcggaa taaaaagaaa aacagaggtc
       61 cacatgaagg atgttgcaac agcgcacccg cctcctccgc cgaggacagc cggtcctgct
      121 cctgtaactg ttacggaaac agcattcgtg acaactcccc caagggcgtg cgctttgatt
      181 gtcgcccttc catccgaaag actggtaata gttgcgccgg tatctgtttt ttcgacgacg
      241 gcaacggttg gattatcaga aaaccagacg agatctttgt tttcggcatg gaggggaaga
      301 gctgtcgccg ctacgggaat tgaagctcgt ccggaaaggg tcgcagaatc gggaaagatc
      361 gcaatttcgg ttacgggtac ccgcggatca ggcgcttcga gcgcggtcgc ggcgtggaga
      421 aaaccgccga agacgacgcg tccctcccaa tggtagttcg ggaccgtatt ccctatgatt
      481 cgcgctttga tctccgccgc gctctttcga gggtatctcg ccgcaagaag cgccgcagtc
      541 ccggcgacgt gcggagaggc catcgatgtg ccgctctccg ttgtgtactc atttcccgga
      601 aatgtgctga gaatatcggt tccgggagcg gccagatgca cgaagttcgg gctgtagttc
      661 gtgaaggatg acggctcacg gtccccggcc atcgagccca cggtgatcat gtggtcgaac
      721 gtgaaacttg cagggtacgg ccgctgcccc cgaagatcca cccagcgaag ctgcgaccag
      781 tcgtagtact cctccggatt gtctatgttc tgcttttcat ttccggcagc gactaccagg
      841 atgatgcctg catcggaaag agccttgcac gcaagggcgt acgggttctt ctcctggtcg
      901 agaatgggtt cgcgccaccc tgtgagcgac atgttcgcca cccggatatt caatccccgc
      961 tctttctgtt caagcagata gttcaagccc gcgatgatcc atgaatcgta tcccaggcct
     1021 tccgcgttga gcaccttcac tccgagcagg ctgacgcgcc agttgacgcc gctcactccc
     1081 agagcgttgt tcccggttgc tccaatgatt cccgcaacat gcgtgccgtg ccagtaatcg
     1141 tccaaagggt ccgcgtcgtc gttgaccgcg tcgaaaccga tgttcccgtc gagatccctg
     1201 ccgatgttgg ccgccaaatc ctcatgattg tagtcgattc cggaatcgat gaccgctaca
     1261 taaatacccg cgtttcccgt tgccgtttcc caagcctccg gagcgcctat atagttcatc
     1321 ccccagagtt tgccgtattt gggatcgttg ggagtgcgtg ttgcgcgtga ttcataattc
     1381 ggccacgccc cgatcacctc gggaagcgct tgcaatgcag cgattagttc ttcagtggtt
     1441 ttgtgctccg aacggatgtg cacgatgtgc ttccctgtat gcgcggcgat ggcgccgtac
     1501 gtccgaacag ccctcgctcc gacggaggcg gcgatgcttt cggcggagga gacgttcgat
     1561 gctgtggata cggggatttg tactaccgcc agtgcctcgc cctccgcata tccatccaaa
     1621 ag
//
LOCUS       DAOTIL010000705         1620 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminobacteriaceae bacterium isolate AMR_MDS_3026
            NODE_90032_length_1620_cov_3.545687, whole genome shotgun sequence.
ACCESSION   DAOTIL010000705 DAOTIL010000000
VERSION     DAOTIL010000705.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868050
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminobacteriaceae bacterium (wastewater metagenome)
  ORGANISM  Aminobacteriaceae bacterium
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae.
REFERENCE   1  (bases 1 to 1620)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1620)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:19:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,224
            CDSs (total)                      :: 3,198
            Genes (coding)                    :: 3,184
            CDSs (with protein)               :: 3,184
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1620
                     /organism="Aminobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_90032_length_1620_cov_3.545687"
                     /isolate="AMR_MDS_3026"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:3034609"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1562)
                     /locus_tag="P5201_12390"
     CDS             complement(<1..1562)
                     /locus_tag="P5201_12390"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HRV99387.1"
                     /translation="MNALKAFSSLNADSPKRTGRMESIHWYVVFVLLLVGAVFLVMEL
                     LHARAVRKQEQFFSDRQYLQTTMSRLALEDRVSSVLMQERFLLEHLLLEPANGSFPPL
                     SDIQRALQSAIYNTADILGFYFAPHPLYPGAQRFSSFRSTPTGDAAREFAGSLEDSFD
                     FSTPRPDDLYHFSGSVRASSRVRMMALVEAAVSEKGFMGYFITIIDLTPFLVRYVLPM
                     AQGAGSAVFLIDREGVVVEHRDSSLVGGNAEKLSGGEGDALRVAWSRVRESFSGMEAI
                     ELFDVSSGMPQRKRLAWNSARVGENRLFVGVYAPESDINPVVSELRVQRFILGGVLAL
                     LLVMIAIAFIRKRSDFEVRRSESTFRAIFNSAASGIAILDANGVILSCNTTMENMTGR
                     PSAQLRMRTLFDLAAPSLQEEKEKVREALRSGALSARAELLFSRKGAEATSPENGSFW
                     GDVSLNRLERDSSTVQMLAVVTDISGLKRAEDLLRQNAETLEARKKELEHVAASQSAL
                     LEMFTFFSECVT"
BASE COUNT          396 a          489 c          424 g          311 t
ORIGIN      
        1 atcgtcacgc actcggaaaa gaacgtaaac atctccagca gggcgctttg actggccgca
       61 acatgctcaa gctccttctt ccgcgcttcc agcgtttccg cgttttgccg gagcaagtcc
      121 tccgcccgtt tcaatcccga aatatcagtc accaccgcga gcatttggac ggttgacgag
      181 tctctttcca ggcggttcag cgaaacatct ccccagaagg atccattctc aggacttgta
      241 gcttcggctc ccttccttga gaaaaggagt tcagccctcg ccgagagcgc gccggatcgc
      301 aaagcctccc gcactttttc cttctcctcc tgcaacgacg gcgccgcgag gtcgaagagc
      361 gtgcgcatcc tcaactgcgc ggaggggcgc cccgtcatgt tttccatcgt cgtattacag
      421 gaaaggataa ccccgttcgc atcgagaatc gctatcccgg acgcggcgct gttgaaaatt
      481 gccctgaatg tcgattcgct ccgccggact tcgaagtcgg accgctttcg aataaaggca
      541 atagcaatca taaccagcaa gagcgcgagt acccctccga gaatgaagcg ctgcacccgc
      601 agttcggaaa ccaccgggtt gatatcggac tcaggagcgt agacgccgac aaacaacctg
      661 ttctccccta ccctggcgga attccatgcc agccgtttcc tctgcggcat cccggaagag
      721 acatcaaaaa gctcgatagc ctccattccg gaaaaagact ctctgacccg cgaccacgcc
      781 acccgaaggg catccccctc tcctcccgag agcttttccg cgttgccccc caccagagaa
      841 gaatcccgat gttcgacaac cactccctcc ctgtcgatga ggaagaccgc gctccctgcc
      901 ccctgggcca tggggagcac atatctgaca aggaaagggg taagatcgat gatcgtgatg
      961 aagtacccca taaagccttt ttccgataca gccgcctcaa ccagcgccat catcctgacg
     1021 cgcgaggacg cccggacgct tcctgaaaaa tggtagagat cgtcgggacg aggagtggaa
     1081 aaatcaaaag aatcttccag agaaccggca aattccctgg ctgcgtctcc ggtcggggtc
     1141 gaacggaacg aactgaaccg ctgcgcgccg gggtacagcg ggtgaggagc aaaatagaat
     1201 cctaggatgt cggcggtgtt gtaaatggcg ctttgaagcg cgcgttgaat gtcggataga
     1261 ggagggaagg acccgtttgc aggctctaga agaaggtgct ccaaaagaaa ccgttcttgc
     1321 atgagaacgg aggagacccg atcctctaac gcgagacgcg acatggtggt ttgaaggtac
     1381 tgacggtcgc tgaaaaactg ttcctgcttg cggactgcgc gggcatggag cagctccatg
     1441 accaaaaata cagcccctac aagcagcaag acgaaaacca cgtaccagtg aatagattcc
     1501 atgcgtccgg tacgcttcgg agaatcggcg ttcaacgagg aaaaagcctt caaagcgttc
     1561 atcctcccag caaccagata cgtgcggcac ttcttcagct cggactccgg tagccattat
//
LOCUS       DAOTIL010000706         1617 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminobacteriaceae bacterium isolate AMR_MDS_3026
            NODE_90270_length_1617_cov_3.069142, whole genome shotgun sequence.
ACCESSION   DAOTIL010000706 DAOTIL010000000
VERSION     DAOTIL010000706.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868050
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminobacteriaceae bacterium (wastewater metagenome)
  ORGANISM  Aminobacteriaceae bacterium
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae.
REFERENCE   1  (bases 1 to 1617)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1617)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:19:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,224
            CDSs (total)                      :: 3,198
            Genes (coding)                    :: 3,184
            CDSs (with protein)               :: 3,184
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1617
                     /organism="Aminobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_90270_length_1617_cov_3.069142"
                     /isolate="AMR_MDS_3026"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:3034609"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..703)
                     /locus_tag="P5201_12395"
     CDS             complement(<1..703)
                     /locus_tag="P5201_12395"
                     /inference="COORDINATES: protein motif:HMM:NF014073.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter permease"
                     /protein_id="HRV99388.1"
                     /translation="MIDFGLLHLLMAFVGVVVGIVFGALPGMTATMAIAVFLPLTYAY
                     DLHVSLFLLLGLYVGGISGGLVPAILVNIPGTPSSICTGFDGYPMAKRGEAVRALRIG
                     ITASLFGGLFSLLLLFLFTPPLARVALSFSSVEKFLIILFALSIIAALSKGNMVTGVF
                     AGFLGVFFSLIGQFADNNRMRMVPDFLSVELRNGFQLLPVLIGLFALVQILQEAEGGM
                     RDDAMGADLSREVRGF"
     gene            complement(720..1175)
                     /locus_tag="P5201_12400"
     CDS             complement(720..1175)
                     /locus_tag="P5201_12400"
                     /inference="COORDINATES: protein motif:HMM:NF018979.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter TctB
                     family protein"
                     /protein_id="HRV99389.1"
                     /translation="MFRVSYKPSTSHWLFPPIILGILALLLAVMIAQRAIECRRTGRP
                     FINREAMRFFEPGCDKLKLFGSVALFVLYIYAMEFAGFLASSIVVVFLYSVLFFGVDR
                     LKELPAAIASGRPWANGAFKSVCVSLLLSVLFSTSVWFLFARVFKITLP"
     gene            complement(1260..>1617)
                     /locus_tag="P5201_12405"
     CDS             complement(1260..>1617)
                     /locus_tag="P5201_12405"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRV99390.1"
                     /translation="VGDKKIAMKFVGLLDNVEGVDVPSYADQGITLADGSEFRFSKDF
                     LIYLPKDFPAEMVKALDDGMKAVSEDPQFKADMAKMSYRGGYLDSAAAKKFIYQKRDS
                     LQGLIDSAPSLDDLVM"
BASE COUNT          380 a          478 c          515 g          244 t
ORIGIN      
        1 tgaagccgcg aacctcgcgg gagaggtcgg cccccatggc gtcgtcccgc atccccccct
       61 ccgcctcctg caatatctgt acgagggcga agagaccgat gagcacggga agcagctgga
      121 agccgttgcg cagctctacg gagagaaagt ccgggaccat gcgcatcctg ttgttgtcgg
      181 cgaactggcc gatcagggag aagaagacgc cgaggaaccc cgcgaagacg cccgtgacca
      241 tgttcccctt ggagagggcg gcgatgatcg agagcgcgaa gagaatgatc aggaacttct
      301 ccaccgagga aaagctgagg gcgaccctgg cgagcggcgg ggtgaagaga aagagcagca
      361 gcaggctgaa gagaccgccg aagagggagg cagtgatgcc gatacggagc gccctgaccg
      421 cctcgccgcg cttggccatc gggtagccgt cgaacccggt gcagatggag gacggggttc
      481 ccggaatgtt cacaaggatg gcgggaacaa gccctccgct gattcccccc acgtagagcc
      541 cgagcaggag aaagagggag acgtgaagat cgtaggcgta cgtgagcgga agaaagaccg
      601 cgatggccat cgtggccgtc atccccggca gcgcgccgaa gacgatgccg acgaccacgc
      661 cgacgaacgc catgagcaga tgaagaaggc cgaaatcgat catgtgcgtt tcctcctctt
      721 tacggcagcg ttatcttgaa cacccgggca aagaggaacc agaccgaggt cgagaaaaga
      781 acggagagga gcagtgagac gcacacggat ttgaatgcgc cgttcgccca cggcctcccc
      841 gaagcgatgg ccgccggaag ctctttgagg cggtcgacac cgaaaaacag cacgctgtac
      901 agaaagacga cgacgatgct cgacgccaga aaaccggcga actccatcgc gtagatatac
      961 aggacgaaga gcgccaccga gccgaacagc ttgagtttgt cgcagccggg ctcgaagaag
     1021 cgcattgctt ccctgttgat aaaaggccgc cctgtcctgc ggcattcgat cgcccgctgc
     1081 gcgatcatca ccgcgaggag cagcgccaga atgcccagga tgatgggcgg aaacagccag
     1141 tgggacgtgg aaggtttgta actcactctg aacatcgctc aatcctccgg aggcagtatc
     1201 gaaagggagc ggcgcgggga aacgaaaagc gttctccgcg ccgctccgtc cgggaagcgt
     1261 tacatcacca ggtcgtcgag ggacggggcg gaatcgatca gcccctgcag ggaatctctc
     1321 ttctgataga tgaacttctt cgccgccgcc gagtcgagat acccgccgcg gtaggacatc
     1381 ttcgccatgt ccgccttgaa ctgcggatcc tcggagaccg ccttcatccc atcgtcgagc
     1441 gccttgacca tctcggcagg gaagtccttc ggaaggtaga tgaggaaatc cttggagaag
     1501 cggaactcgc tcccgtcggc aagcgtgatc ccctggtcgg cgtacgaggg aacgtcgacc
     1561 ccctccacgt tgtccagaag gccgacgaac ttcatggcga tcttcttgtc cccgacc
//
LOCUS       DAOTIL010000707         1617 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminobacteriaceae bacterium isolate AMR_MDS_3026
            NODE_90290_length_1617_cov_2.422535, whole genome shotgun sequence.
ACCESSION   DAOTIL010000707 DAOTIL010000000
VERSION     DAOTIL010000707.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868050
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminobacteriaceae bacterium (wastewater metagenome)
  ORGANISM  Aminobacteriaceae bacterium
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae.
REFERENCE   1  (bases 1 to 1617)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1617)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:19:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,224
            CDSs (total)                      :: 3,198
            Genes (coding)                    :: 3,184
            CDSs (with protein)               :: 3,184
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1617
                     /organism="Aminobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_90290_length_1617_cov_2.422535"
                     /isolate="AMR_MDS_3026"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:3034609"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>1617
                     /locus_tag="P5201_12410"
     CDS             <1..>1617
                     /locus_tag="P5201_12410"
                     /inference="COORDINATES: protein motif:HMM:NF019344.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit"
                     /protein_id="HRV99391.1"
                     /translation="AGVREFLLALCRSSRIEDELFAAAGVRDRGALLDRMLAEQKEKE
                     QKEARHRAADSVIGRNGRDLTALAKAGKLHMPLMRDTECAQTINILCRMEKGNPILLG
                     EAGTGKTAIAEGVACLVASGNVPQTLRGARVVEISASAFTDAFRKGMGEEFVRRLAKE
                     TLADDESGKRSILFVDELHILLEVGAMSEFLKPFLARGGFRMIGATTFKEYRRFIERD
                     EAFSRRFAPVHVEEFTPEQAIKVITGRLSRLEAYHGVRITPEAAEASVRLSHRYIPHR
                     RLPDKALDILDTACVLALSGGEVTQGHIKAAVASAANLPVEAVSNSFLETCKGLSKRL
                     SGRVKGQRAAVGAVSRLLKRCASGLGGEGNRPLGSFLFAGSTGTGKTELAKAVAESFF
                     GREDKMIRLDMSEYCEPSAVNRLIGSSPGYVGSEQGGVLTEAIRKNPFSLILVDEIEK
                     AAPQVVKLFLQILDEGRLSDASGNSFDFRNALIVFTTNAGAHEKRSSIGFLSEGEQKE
                     QARETLHENMGHVFPREFLGRFDEVIHFDDL"
BASE COUNT          305 a          548 c          511 g          253 t
ORIGIN      
        1 gccggagttc gcgaattcct tctcgcgctc tgccgcagca gccggatcga agacgagctc
       61 ttcgccgccg ccggagtgcg cgaccgtggc gcccttctcg accggatgct cgccgagcag
      121 aaggaaaagg agcagaagga agcccgccac cgcgccgcgg actccgtcat cggacgcaac
      181 ggacgcgacc tcaccgccct cgcgaaggcg ggaaagctgc acatgcccct gatgcgcgac
      241 accgagtgcg cacagacgat caacatcctc tgcaggatgg aaaaggggaa ccccattctg
      301 ctcggcgagg ctggaacggg caagaccgcc atcgccgaag gagtcgcctg cctcgtcgca
      361 tccggaaatg tgccgcagac gctgcgcggc gcgcgcgtgg tcgaaatatc cgcctccgcg
      421 ttcaccgacg ccttccgcaa agggatgggc gaggagttcg ttcggcggct cgccaaggag
      481 actcttgccg acgacgagag cggcaagcgc agcattctct tcgtggacga gctgcacatc
      541 ctgctcgaag tgggcgctat gagcgaattc ctcaagccct tcctcgcgcg cggaggtttc
      601 cgtatgatcg gggccaccac gttcaaggag taccgccgtt tcatcgagcg ggacgaggcg
      661 ttctcccgcc gcttcgctcc cgtccacgtc gaggagttca ccccggagca ggcgatcaag
      721 gtgataacgg ggcggctttc ccgcttggag gcataccacg gagtgcgcat cacccccgaa
      781 gctgcggagg cctccgtccg cctttcgcac cgctacatcc cgcaccgccg ccttccggac
      841 aaggcgctcg acatactgga cacggcctgc gtcctggctc tgtccggcgg ggaagtcacg
      901 caggggcaca tcaaggccgc cgtcgcctcg gcggcgaatc tgccggtcga agccgtcagc
      961 aacagcttcc tcgagacgtg caagggactg tcgaaacgcc tctccggaag ggtgaagggg
     1021 cagcgggctg ccgtcggcgc ggtctcgcgc ctgctcaaac gctgcgcgtc gggcctcggc
     1081 ggggaaggga accgccccct cggcagcttc ctcttcgccg gatccaccgg aaccggcaag
     1141 accgaactcg ccaaggccgt ggctgagtcc ttcttcggcc gagaggacaa aatgatccgc
     1201 ctcgacatga gcgaatactg cgaaccgtcc gccgtgaacc gcctgatcgg ttcttccccc
     1261 ggctacgtcg gctccgagca ggggggcgtg ctcaccgagg cgatccgcaa gaaccccttc
     1321 agcctcattc ttgtggacga gatcgagaag gcggccccgc aagtggtcaa gctcttcctc
     1381 cagatactcg acgagggcag gctgagcgac gcctcgggga actccttcga cttccgcaac
     1441 gcgctcatcg tcttcaccac aaacgccggc gcgcacgaga aacgaagctc aatcggcttc
     1501 ctctccgaag gcgagcagaa ggagcaggcg cgggagacgc tgcacgagaa catggggcat
     1561 gtctttccga gggagtttct ggggcgcttc gacgaggtga tccacttcga cgacctc
//
LOCUS       DAOTIL010000708         1613 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminobacteriaceae bacterium isolate AMR_MDS_3026
            NODE_90603_length_1613_cov_2.349807, whole genome shotgun sequence.
ACCESSION   DAOTIL010000708 DAOTIL010000000
VERSION     DAOTIL010000708.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868050
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminobacteriaceae bacterium (wastewater metagenome)
  ORGANISM  Aminobacteriaceae bacterium
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae.
REFERENCE   1  (bases 1 to 1613)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1613)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:19:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,224
            CDSs (total)                      :: 3,198
            Genes (coding)                    :: 3,184
            CDSs (with protein)               :: 3,184
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1613
                     /organism="Aminobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_90603_length_1613_cov_2.349807"
                     /isolate="AMR_MDS_3026"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:3034609"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1597)
                     /locus_tag="P5201_12415"
     CDS             complement(<1..1597)
                     /locus_tag="P5201_12415"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HRV99392.1"
                     /translation="MQQTRGLWAFGLWLRIFSIVAASRLWLASSPPPLLIRSLLASSL
                     PASFAFLLASPLLLTEQIKARIAQIQEAQLARGVPVHGSFRERASSLTALLFPLVLGL
                     LNDLPSRSAALDMKAFGLFRRRSSLFGGREPRVPRTPRPVSRDGHGETSRTGESEKAL
                     GGSGGGDVFREPAEIAENAESRCASASPDFAPIPEAFRPPEAANSPGALPFLALDDAR
                     FTAPGAEAPIVTVPALRLFPGEWMLLEGGNGAGKSTLATMLCGGVPEHRSGILSGRAE
                     LCGEPVASRTSLQWSPDVQLVQQNPQLSLSGCAFTVYEEVAFGPENLALPLGEIRRRV
                     DDALELFGITHLKEHHPATLSGGEAQKAALASAAAMRPRLLLLDEAFSRIASADVPVL
                     LERLRAWSSKHSTAVLLLERRGQSFERFCDRFGTFLDGVVLERDEETVGRSAAGRSRP
                     EAAPSVVRGDTESFCAGKRRKDDDRPSFGNVILEQDDSGESACMDRFSKTSAAKEGAA
                     LLREESPALLRILDLDFRWKSRSE"
BASE COUNT          291 a          543 c          544 g          235 t
ORIGIN      
        1 gctcgctccg gctcttccaa cggaagtcga ggtccagaat ccgaagaagg gcgggactct
       61 cttcgcggag aagcgcagct ccctccttcg cggcgctcgt cttcgagaag cggtccatgc
      121 acgcgctttc tccggaatcg tcctgctcaa gaatgacgtt tccgaaagat ggccggtcgt
      181 cgtctttgcg tcgcttcccg gcgcagaaac tctccgtgtc gccgcgaaca accgacggag
      241 ccgcttccgg tcgtgaacgc ccggcggcgc tcctcccgac cgtttcttca tcgcgttcca
      301 gcacgacgcc gtccaggaaa gtaccgaagc ggtcgcagaa gcgctcgaag gactgtccgc
      361 gccgttcgag cagaagcacc gcagtactat gctttgacga ccacgcccgc agtcgctcca
      421 gcagcacggg cacatcggcg gaggcgatcc ggctgaacgc ctcgtcgagc agcagcaggc
      481 gggggcgcat cgccgccgcg gaggccaggg cggccttctg cgcctcccct ccggagagag
      541 tcgcagggtg atgttccttg agatgcgtta tcccgaacag ctccagcgcg tcgtcgacgc
      601 ggcggcggat ctcgccgagg gggagcgcaa ggttctccgg cccgaaggcg acctcctcgt
      661 acaccgtgaa ggcacacccc gaaagggaga gctgagggtt ctgctggacg agctgcacgt
      721 ccggcgacca ctggagactc gtccgggagg cgaccggttc gccgcacagc tccgcgcgcc
      781 ccgaaaggat gccgcttctg tgctcgggaa ccccgccgca gagcatcgtc gccagcgtgg
      841 actttccggc gccgtttccg ccttcgagca gcatccattc gccggggaag aggcgcagcg
      901 cgggaaccgt cacgatcggg gcctccgcgc cgggcgcggt gaaacgcgcg tcgtcgagag
      961 cgaggaaggg aagcgcaccg ggagagttcg cagcctccgg aggacgaaag gcttcgggga
     1021 tcggtgcgaa atccggcgaa gcggaggcgc agcggctctc cgcattttct gcaatttccg
     1081 ccggttcgcg gaacacatcg cctccgcccg atcctccgag cgctttctcc gactcgccgg
     1141 tccgggacgt ttcaccgtgt ccgtcgcggg ataccggcct cggagtgcgc gggacgcgcg
     1201 gctccctgcc tccgaaaagg ctcgaacgcc gccggaacag cccgaacgcc ttcatgtcga
     1261 gcgcggcgct ccgtgagggg aggtcgttca gcaaccccaa caccagcggg aagagcagcg
     1321 ccgtcagcga ggaggcccgt tcgcggaaag aaccgtgcac cgggacgccg cgcgcgagct
     1381 gcgcctcctg aatctgggcg atccgcgcct tgatctgctc ggtcagcagc agcgggctcg
     1441 ccagcaggaa ggcgaagctc gccggaagac tgctcgccaa aagcgagcgg atgagtaacg
     1501 ggggaggaga ggaggcgagc cagaggcggc ttgccgcgac gatggagaag atgcggagcc
     1561 aaagcccgaa cgcccacagt ccgcgagtct gttgcacaac gccgccaatc agc
//
LOCUS       DAOTIL010000709         1610 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminobacteriaceae bacterium isolate AMR_MDS_3026
            NODE_90849_length_1610_cov_2.475884, whole genome shotgun sequence.
ACCESSION   DAOTIL010000709 DAOTIL010000000
VERSION     DAOTIL010000709.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868050
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminobacteriaceae bacterium (wastewater metagenome)
  ORGANISM  Aminobacteriaceae bacterium
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae.
REFERENCE   1  (bases 1 to 1610)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1610)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:19:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,224
            CDSs (total)                      :: 3,198
            Genes (coding)                    :: 3,184
            CDSs (with protein)               :: 3,184
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1610
                     /organism="Aminobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_90849_length_1610_cov_2.475884"
                     /isolate="AMR_MDS_3026"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:3034609"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..568)
                     /locus_tag="P5201_12420"
     CDS             complement(<1..568)
                     /locus_tag="P5201_12420"
                     /inference="COORDINATES: protein motif:HMM:NF024140.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MCP four helix bundle domain-containing protein"
                     /protein_id="HRV99393.1"
                     /translation="MNVFANMKTKGKILALVLVMALLLGAVGYTGYYFNAQSAENLSE
                     VYEENLLPITWLFDALVQGQGIQSDVAQILLAHDAESREELEADIVRRVAIVDEHYAR
                     YGKLPLDAFEKEHFELALKALADYRVAWRKSKDLAAADRGYEAYQTFTTEVNPLFTAY
                     QAEIRSLAEYAQKRADETNASIIPTHTIV"
     gene            complement(731..>1610)
                     /locus_tag="P5201_12425"
     CDS             complement(731..>1610)
                     /locus_tag="P5201_12425"
                     /inference="COORDINATES: protein motif:HMM:NF024989.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="zinc-binding alcohol dehydrogenase family
                     protein"
                     /protein_id="HRV99394.1"
                     /translation="GTNPLAKYPRIIGHEFAGEIAALGEGVEGFSVGDRVTVDPVTSC
                     GECYACSIGRRNVCERLEVFGVHRDGGFAEYVALPASNLHKVPASWSFEKAALVEPFT
                     IAANVLSRTECAGSDKLLIMGAGPIGQVILQAARRLGTACAVSDIVGVRLEKARRLGA
                     ELTVNPKETSLEEAIGAWTCGKGVPLIIDAVGAPDLFPSLLRMASPAGRIGLLGFSKE
                     PSPFVQLEAVRKELSIFGSRLNRNKFPEVISWFEEGLVEPELLVSHRFPFVQAKEAME
                     FIQEHPSDVCKVILEF"
BASE COUNT          325 a          515 c          448 g          322 t
ORIGIN      
        1 ggactatcgt gtgggtcggg attatactcg cgttcgtctc gtccgccctc ttctgcgcat
       61 actccgcaag cgagcgaatc tccgcctggt atgcggtaaa gagcgggttc acttcagtgg
      121 tgaacgtctg gtatgcttcg taccccctgt cggcggcggc aagatccttg gacttgcgcc
      181 acgcgaccct gtagtcggcc agcgccttca gggcgagctc gaaatgttct ttctcgaagg
      241 cgtcaagagg aagcttgccg tacctggcgt agtgctcgtc gacgatggcg acccgccgca
      301 cgatatctgc ttccagttcc tcccgggact ccgcatcgtg cgccaacaat atctgcgcaa
      361 cgtcgctctg aataccctgc ccttgaacca gggcgtcgaa gagccaggtg atcgggagca
      421 aattctcctc atacacctca gagaggtttt cagcgctctg tgcattgaaa tagtaaccgg
      481 tgtaccccac cgcacccagt agaagggcca ttacgagcac aagcgctaag atttttccct
      541 tggttttcat atttgcaaac acgttcatac acaactctcc tcgttaaaga aatagaattc
      601 cggttgaaag catacgctgc atgattgatt atcctcgttt tctctttcgc aggcaacccc
      661 gaagaaacgc atgaaaaaag acgcccttcg gcacagcgcc taagggtgtc ttcattcccg
      721 cttggtattt ttagaactcc agaatcacct tgcagacatc ggaggggtgc tcctgaatga
      781 actccattgc ctccttcgcc tgtacgaacg ggaagcggtg cgagacgagc agttccggct
      841 cgacaagacc ttcctcgaac caggagatga cttccgggaa cttgttccgg ttgagccggg
      901 agccgaagat cgacagctcc tttcgcacag cttcgagctg cacaaacggc gagggctcct
      961 tcgagaagcc gagcagtccg atgcgccccg ccggggaggc catccgaagc agcgagggga
     1021 agagatccgg cgcccctacc gcgtcgatga tgagcgggac gcccttcccg caggtccacg
     1081 ctcctatcgc ctcttcaagc gacgtctcct tcggattgac ggtgagctcg gcgcccagac
     1141 gacgggcctt ctccaggcgg acgccgacga tgtcggacac ggcgcacgcc gtcccgagcc
     1201 gccttgcagc ctggaggatc acctggccga tcggccccgc ccccatgatc agcagtttgt
     1261 cgcttcccgc gcactccgtc ctggagagga cgttcgccgc gatggtgaac ggctcgacga
     1321 gcgcggcctt ctcgaaactc catgaagcag gaactttgtg aaggttcgag gcaggaagcg
     1381 caacgtattc ggcaaaaccg ccgtccctgt gcacgccgaa gacctccagg cgctcgcaga
     1441 cgttgcgccg cccgatggaa caggcatagc actctccgca gctcgtcaca ggatcgaccg
     1501 tgaccctgtc gccgacggag aacccctcaa cgccctcccc gagcgccgcg atctcccccg
     1561 cgaactcgtg ccctatgatc cgggggtact tcgcaagggg gttggtcccg
//
LOCUS       DAOTIL010000710         1608 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminobacteriaceae bacterium isolate AMR_MDS_3026
            NODE_90984_length_1608_cov_2.571153, whole genome shotgun sequence.
ACCESSION   DAOTIL010000710 DAOTIL010000000
VERSION     DAOTIL010000710.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868050
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminobacteriaceae bacterium (wastewater metagenome)
  ORGANISM  Aminobacteriaceae bacterium
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae.
REFERENCE   1  (bases 1 to 1608)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1608)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:19:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,224
            CDSs (total)                      :: 3,198
            Genes (coding)                    :: 3,184
            CDSs (with protein)               :: 3,184
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1608
                     /organism="Aminobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_90984_length_1608_cov_2.571153"
                     /isolate="AMR_MDS_3026"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:3034609"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            171..1097
                     /locus_tag="P5201_12430"
     CDS             171..1097
                     /locus_tag="P5201_12430"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRV99395.1"
                     /translation="MISCEFFDDLLAAALPELLEAMDTDKRPEFQEQELRAVATTTGG
                     KERNIDLLAKVALKSGKNVHVLLHIEIQDSDDDDFPERMFRYRSLLMARYLKSYEDKG
                     RAVLVNDKAEDIVSLAILTARRPKREKEYYEHSSFRNTLRFEYPALKVWECDPAEMAN
                     SENPFHWVLLSGRRVLESGRNDAKKVRYLRELNDILKEKRWSRRKKERLFIFMEALLR
                     PKEEEALKEYRKYLEEVKTKEGEKMAYVSVPEEMGIEKGIEKGIVIGAEGTKRENALR
                     MLRKGLDIDLIAECVDLPEEEVRRLADENIHD"
     gene            complement(1164..1328)
                     /locus_tag="P5201_12435"
     CDS             complement(1164..1328)
                     /locus_tag="P5201_12435"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRV99396.1"
                     /translation="MLRTGMGASFATECGDLQEDKVARLAEEARRYVRLTTGARSRET
                     GKNTEAARGA"
BASE COUNT          389 a          451 c          459 g          309 t
ORIGIN      
        1 gcctcattga aaaacaccgt ttgaacttct ctgtcggcaa taaccttttg ttgccggccg
       61 tgacgttgct aagcggttcc cttatttgaa atcactgctg tccaaaatcg cgattcagac
      121 ccctgcaaac accggaagca atttcttgac gtgagttatt ttggacagca gtgatctcgt
      181 gcgagttctt cgacgacctg ctggcagcgg cgcttcccga gcttctggag gcgatggaca
      241 ccgacaaaag acccgagttt caggagcagg agcttcgcgc cgtcgcgacg acgaccggcg
      301 ggaaggagcg gaacatcgac cttctggcga aggtcgcgct gaagtcgggg aagaacgtcc
      361 acgtgctgct ccacatcgag atacaggaca gcgacgacga cgactttccc gagaggatgt
      421 ttcgttacag atcgcttctc atggcgcgct acctcaagag ctacgaggac aagggcaggg
      481 cagtgctcgt caacgacaag gccgaggaca tcgtctccct ggcgatactc accgcgcgcc
      541 gcccgaaaag agagaaggag tactacgaac attcctcctt ccgcaacacg ctgcgattcg
      601 aataccccgc gctcaaggtc tgggagtgcg accccgccga gatggcgaac tccgagaacc
      661 ccttccactg ggtgctgctg tccgggcgaa gggttctcga aagcgggcgg aacgacgcga
      721 agaaggtgcg ctacctgcgg gagctgaacg acatcctgaa ggagaagcgg tggtcccggc
      781 gcaagaagga gcggctcttc atcttcatgg aggcccttct tcgccccaag gaagaggaag
      841 ccctcaagga gtaccggaag tacctcgaag aggtcaagac gaaggaggga gagaagatgg
      901 catatgtgag cgttcccgaa gagatgggaa tcgaaaaggg aatcgaaaag gggatcgtca
      961 tcggagcgga aggaaccaag cgcgagaacg cccttcggat gctgcgcaag gggctggaca
     1021 tcgacctcat cgccgagtgt gtcgacctcc ccgaggagga agtacgtcgg ctcgccgacg
     1081 aaaatataca cgactgaccc tcggacatcc cccgatcagc aacaaccatg accggaggcg
     1141 atacggagga tatcggcgac gcttcacgct ccccgtgcgg cctccgtgtt cttccccgtt
     1201 tcgcgcgagc gcgcccccgt cgtgagtcga acgtatcggc gcgcctcttc ggcgaggcga
     1261 gcgaccttgt cctcctggag gtcgccgcac tccgttgcaa aggaggcgcc catcccagtg
     1321 cgcaacatcc tgagagcatg ctctcgctgc acgctttcca tgataaagtg tatgaaagct
     1381 tccgcctcga gtcgcaaaac ccgcaaaaac cgaccgtttt tcgattttca aattacaaca
     1441 tgcctggaca gggtataaag tagcatttat aagggtaaag cgtatttgtc cgcgtattga
     1501 ttctatatta gactaacgcg ctcctattac ataaacgggg ttttttcgaa tcaatcaaat
     1561 gtgtcattat agggtacacg aagctcttca acagagcatt cgaagccg
//
LOCUS       DAOTIL010000711         1607 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminobacteriaceae bacterium isolate AMR_MDS_3026
            NODE_91063_length_1607_cov_2.623711, whole genome shotgun sequence.
ACCESSION   DAOTIL010000711 DAOTIL010000000
VERSION     DAOTIL010000711.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868050
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminobacteriaceae bacterium (wastewater metagenome)
  ORGANISM  Aminobacteriaceae bacterium
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae.
REFERENCE   1  (bases 1 to 1607)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1607)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:19:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,224
            CDSs (total)                      :: 3,198
            Genes (coding)                    :: 3,184
            CDSs (with protein)               :: 3,184
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1607
                     /organism="Aminobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_91063_length_1607_cov_2.623711"
                     /isolate="AMR_MDS_3026"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:3034609"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1017
                     /gene="lsrB"
                     /locus_tag="P5201_12440"
     CDS             <1..1017
                     /gene="lsrB"
                     /locus_tag="P5201_12440"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF011937.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autoinducer 2 ABC transporter substrate-binding
                     protein LsrB"
                     /protein_id="HRV99397.1"
                     /translation="CDPSVPAVVVPAAAAVAAPKDVSVVFIPKLTGNMFFESAAEGAL
                     AMAKEVGFECKYDGSPEASVANQVQIINSAVNQGFNAISVSSLSPTGLSQALQNAKKR
                     GVKVVTWDSDVDPKDRAIYVNQGTPEILGAMLVDMAYDQMNDEQKKSAKFAFFYSSPT
                     VTDQNAWVDFAKKYIAEKHPGWEMLTVQYGEQDAQKSLQIGQSILQTYPDIDAIICPD
                     STALPAMAQAAKNLEKAGKVIITGFATPSSMRGFVKDDTVKAFGLWDCKLQGAIGAYI
                     AYWLADGNTFKVGDFIDVPGVGKLKVEPNSIQGYDYEAEDSGIVLLPERVVFTKENID
                     NYNF"
     gene            1193..1594
                     /locus_tag="P5201_12445"
     CDS             1193..1594
                     /locus_tag="P5201_12445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013047926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019239 - deaminase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rid family detoxifying hydrolase"
                     /protein_id="HRV99398.1"
                     /translation="MKPTALSSSRAPAPKGFYSQAVRVGSLLFTAGQLPVDPESGDFV
                     RGTVEAQTERVIENIRGILEENGSSLSLVVKATVYIRDIASWGSVNSVYSRYFEGTVP
                     PARTVVGGVDIHYGLDVEMDVVAYVEETGQE"
BASE COUNT          317 a          528 c          489 g          273 t
ORIGIN      
        1 tgtgacccgt cagtcccagc ggtcgtcgtc ccagcggcgg ctgcggttgc ggcgccgaag
       61 gatgtcagcg ttgtctttat tcccaagctc accggaaaca tgttcttcga gtcggcggcc
      121 gagggcgcgc tggccatggc caaggaagtc ggcttcgagt gcaagtacga cggctccccc
      181 gaggcgtccg tcgccaacca ggtgcagatc atcaacagcg ccgtgaacca gggcttcaac
      241 gccatctccg tctcctcgct ctcccccacg gggctgagcc aggcgctgca gaacgcgaag
      301 aagcgcggcg tcaaggtcgt gacgtgggat tccgacgtcg accccaagga ccgcgccatc
      361 tacgtcaacc aggggacgcc cgagatcctc ggcgcgatgc tcgtcgacat ggcctatgac
      421 cagatgaacg acgagcagaa gaagagcgcg aagttcgcct tcttctactc cagcccgacc
      481 gtcaccgacc agaacgcatg ggtcgacttc gcgaagaagt acatcgccga gaagcacccc
      541 ggctgggaga tgctgacagt tcagtacggc gagcaggacg cgcagaagtc ccttcagatc
      601 ggccagtcca tccttcagac ctaccccgac atcgacgcga tcatctgccc cgactccacc
      661 gctcttcccg cgatggcgca ggccgccaag aaccttgaga aggccggaaa ggtcatcatc
      721 accggattcg ccacccccag ctcgatgcgc ggcttcgtga aggacgacac cgtcaaggcg
      781 ttcggcctgt gggactgcaa gctccagggc gcgatcggcg cgtacatcgc ctactggctg
      841 gccgacggca acaccttcaa ggtcggcgac ttcatcgacg tccccggcgt gggcaagctg
      901 aaggtcgagc ccaactccat ccaggggtac gactacgagg ccgaggactc cggcatcgtc
      961 ctgctccccg agcgggtcgt cttcaccaaa gagaacatcg acaactacaa cttctaacca
     1021 tcgctgcacg gcttcgccac gcgctcccgg cgcctcgggg gcgcgttttt catacccggc
     1081 aagccccaag cgttcccggg ccgccgcctt accgtctgct cttgcgtccc ggctccggaa
     1141 atgtcatact caattggtac aagtatattt ttctctcggg agggaatact gaatgaagcc
     1201 tacagcactc tcctcgtcgc gggcgccggc ccccaagggg ttctactcgc aggccgtccg
     1261 cgtcggatcg ctcctcttca ccgcggggca gctccctgtg gacccggaaa gcggcgactt
     1321 cgtccgcggc acggtggaag cgcagaccga acgcgtcatc gagaacatcc gcgggattct
     1381 ggaggagaac ggctcctccc tgtcgctcgt agtcaaggct acggtctaca tccgcgatat
     1441 agcttcgtgg gggagcgtga acagcgtgta cagccgctat ttcgagggaa ccgtgcctcc
     1501 cgccagaaca gtggtcggcg gcgtggacat ccactacggg ctcgatgtgg agatggacgt
     1561 cgtggcgtac gtggaggaga cggggcagga gtagcagagg ggaggaa
//
LOCUS       DAOTIL010000712         1604 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminobacteriaceae bacterium isolate AMR_MDS_3026
            NODE_91300_length_1604_cov_2.701097, whole genome shotgun sequence.
ACCESSION   DAOTIL010000712 DAOTIL010000000
VERSION     DAOTIL010000712.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868050
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminobacteriaceae bacterium (wastewater metagenome)
  ORGANISM  Aminobacteriaceae bacterium
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae.
REFERENCE   1  (bases 1 to 1604)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1604)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:19:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,224
            CDSs (total)                      :: 3,198
            Genes (coding)                    :: 3,184
            CDSs (with protein)               :: 3,184
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1604
                     /organism="Aminobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_91300_length_1604_cov_2.701097"
                     /isolate="AMR_MDS_3026"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:3034609"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..179
                     /locus_tag="P5201_12450"
     CDS             <1..179
                     /locus_tag="P5201_12450"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013700.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="M23 family metallopeptidase"
                     /protein_id="HRV99399.1"
                     /translation="CSRLHVKRGQKVHQGQVIAAVGETGRATGPHLHFEIRTGGGAVN
                     PLEFWRKHAESPAQ"
     gene            complement(236..1558)
                     /locus_tag="P5201_12455"
     CDS             complement(236..1558)
                     /locus_tag="P5201_12455"
                     /inference="COORDINATES: protein motif:HMM:NF022655.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LmeA family phospholipid-binding protein"
                     /protein_id="HRV99400.1"
                     /translation="MRKTFLLFVCLLLFNPFPAVAEQTFREFSGEFTPQNNGERLLAF
                     LVSVTDPESLELQMDALPGDDGAIRAVSFALHGGALGGFRIERLTLESAFIRLNPPSE
                     WIRGNRHSLRVCNALRSNFELVIDEHDILEALKKYASGSWSRISVDLTSGELRIQGRY
                     KPGATGFSLMAGITTRLELRDGKRVMLKAPEIRINGENKTSLFQRDLERLRPVLDFNG
                     FPPALTLSRLSVDKGKLLLATATRPKAVGGIRYRYVRDRERPFSIHPPESFDFAERQF
                     RNGDIVLVGGKTWRSKIVNLFEDGNAAFSHTGIVRIVGGVPYIIHASPESELVRMERA
                     ETFLSPKKVDRASIYRLRGNPGAAETAGRKALEYYEERVPFDTAFDSEDHERLYCTEL
                     IWRAYTHAGIDLADGLWYSLYNPVLSGRLLLPNSLTRSPLLEETAILR"
BASE COUNT          317 a          458 c          506 g          323 t
ORIGIN      
        1 actgctcccg tcttcacgtc aagcgagggc agaaggtgca tcaaggacag gtaatcgcgg
       61 cggtcggcga gacgggaaga gcgacggggc cgcaccttca tttcgagata cgcacgggcg
      121 gcggcgcggt caatcctttg gagttctgga ggaagcacgc ggaatcgccg gcgcagtgaa
      181 aaccacagtg cggccgttca catcggacat cgaagcgccg cgccgccctc ccggtttacc
      241 gcaggatcgc cgtttcttcg agcagcgggc ttcgggtcag gctgttcgga agcagcagtc
      301 tgccggagag cacagggttg tacagggagt accacagtcc gtcggccagg tcgatacccg
      361 cgtgcgtgta cgccctccag ataagctcgg tgcagtaaag cctttcgtgg tcttcgctgt
      421 cgaacgcggt gtcgaacggc acgcgctctt cgtagtactc aagggctttt ctgccggctg
      481 tttcagcggc gcccggattc ccccgcagac ggtagatact tgccctgtcc acttttttcg
      541 gggataaaaa agtctccgcg cgttccattc gcaccagctc ggactcggga gatgcatgga
      601 tgatgtacgg gacgccgccg actatcctga cgatgccggt atgtgaaaag gcggcgtttc
      661 cgtcttcaaa aagattgacg atcttgctcc gccacgtttt cccgccgacg agtacgatat
      721 ctccgttccg gaactgccgc tcggcgaagt cgaacgactc cggcgggtga atggagaaag
      781 gtctttctcg gtcgcggacg tagcggtagc gtatgccgcc gaccgccttg ggtcgtgtcg
      841 cggtcgccag aagaagcttc cctttgtcga cggagaggcg tgagagcgtc agcgccggag
      901 gaaaaccgtt gaagtcgaga acggggcgga gccgttcgag atctctttgg aaaagagatg
      961 tcttgttctc gccgttgatg cgtatctccg gagctttcag catgacgcgc tttccgtcgc
     1021 gcagttcgag cctcgtcgtt attccggcca tgagggagaa gcctgtcgca ccgggcttgt
     1081 agcgcccctg tatgcgaagt tcgcccgacg tcaggtccac ggagatgcgg ctccagctcc
     1141 ccgaggcgta tttcttcagg gcttcgagga tatcatgttc gtcgatcaca agttcgaagt
     1201 tgctccggag cgcgttgcat acccggagcg aatgccggtt gccgcgaatc cactcagagg
     1261 gcggattgag ccgtataaat gccgactcta gggtcagccg ttctatgcgg aaccctccga
     1321 gagcgccgcc gtgaagagca aaagaaacgg cccggatggc gccgtcgtct cccggaagcg
     1381 catccatttg tagctccagc gactccgggt cggtgaccga gaccagaaac gcgagcagcc
     1441 gctctccgtt gttttggggg gtgaactcgc cggaaaactc gcgaaaggtc tgctccgcga
     1501 cggctgggaa cggattgaag agcaaaagac atacgaagag aaggaatgtt ttcctcacgc
     1561 tgatcccccc tttcgatgca gtgaaaaagc ggacggcttc gcgt
//
LOCUS       DAOTIL010000713         1603 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminobacteriaceae bacterium isolate AMR_MDS_3026
            NODE_91345_length_1603_cov_7.817183, whole genome shotgun sequence.
ACCESSION   DAOTIL010000713 DAOTIL010000000
VERSION     DAOTIL010000713.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868050
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminobacteriaceae bacterium (wastewater metagenome)
  ORGANISM  Aminobacteriaceae bacterium
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae.
REFERENCE   1  (bases 1 to 1603)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1603)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:19:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,224
            CDSs (total)                      :: 3,198
            Genes (coding)                    :: 3,184
            CDSs (with protein)               :: 3,184
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1603
                     /organism="Aminobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_91345_length_1603_cov_7.817183"
                     /isolate="AMR_MDS_3026"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:3034609"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1548
                     /gene="istA"
                     /locus_tag="P5201_12460"
     CDS             <1..1548
                     /gene="istA"
                     /locus_tag="P5201_12460"
                     /inference="COORDINATES: protein motif:HMM:NF033546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS21 family transposase"
                     /protein_id="HRV99401.1"
                     /translation="KGREWRMLAMTRINDIRKAYFDEGCTISAIAAKFGIDRKTARKY
                     IEQDDFNKVPPVQSKRNRWPKLEPYMKRIDEWLEDDVKDRSKQRHTAKRVHARLEGTY
                     PDFDCSYRTVASYVKEKKREIYSDVRAALPLEHKAGEAQVDFGSADFYENGTLCSGKY
                     LTVSFPYSNAGYLQLFKGENRECLFEGLKAIFEHLGGVPTRLWFDNMSTVVKKVLKGG
                     KRELCDAFLRFKEHHGFDAAFCNANAGHEKGNVENKVGYHRRNLLVPIPEFERLDRYN
                     EELLRLCDEDHRREHYKRNSTIEALHREDKETLRPLPAVEFDCASYETMKTDLWGKFR
                     ISGVHTYSTAPKHAGGRVLVRFTANSVVPLDENHRPITTHRRLYGSLKQEEMDWLPYL
                     TQLSRNPGAMKYSGIWEMLPDDLRGYLEGKEKSGQRDVLRTLALLSERDGFERAVKSV
                     SEALRRGIEDLDSLVALHGHLHGEYPADSMRIDETKLGSLPKLPEVRFAAKVYDGFLS
                     AGGRLPC"
BASE COUNT          401 a          438 c          509 g          255 t
ORIGIN      
        1 aaggggaggg aatggaggat gctcgcaatg acccgcatca atgatatcag aaaagcgtat
       61 ttcgacgagg gatgcaccat cagcgccatc gcggcgaagt tcggaatcga ccggaagacc
      121 gcgagaaagt acatagaaca ggacgacttc aacaaagttc ctcccgttca gtccaaaagg
      181 aatcgttggc cgaagctgga gccctacatg aagcggatcg acgagtggct cgaagacgac
      241 gtcaaggatc ggagcaaaca gcggcacacg gcgaagaggg tgcacgcacg gctggagggg
      301 acatatccgg atttcgactg ctcttaccgg accgttgcct cctacgtgaa ggagaagaag
      361 cgggagatct actccgacgt ccgcgccgct ctcccgttgg agcacaaagc tggagaggcg
      421 caggtggatt tcggaagcgc cgatttctac gaaaacggga cgctctgctc ggggaagtat
      481 ctcaccgttt ccttccccta cagcaacgca ggatatctcc agctcttcaa gggtgagaac
      541 cgggaatgcc tcttcgaggg tctgaaggcg atcttcgagc atctgggagg ggtgccgacg
      601 cggctgtggt tcgacaacat gtcgaccgtc gtgaagaagg tgctcaaggg aggcaagcgc
      661 gagctgtgcg acgccttcct ccgcttcaag gagcaccacg gcttcgatgc ggccttctgc
      721 aacgcgaacg cggggcatga aaaagggaac gttgagaaca aggtcgggta ccacagacgc
      781 aaccttctcg tgccgatccc cgagttcgag agactggacc ggtacaacga ggagcttctg
      841 cggctctgcg acgaggacca ccgcagggag cactacaaga gaaacagcac catagaggcg
      901 cttcacaggg aggacaagga aacgctgcgc ccgctccccg ccgtcgagtt cgactgcgcg
      961 agctacgaga cgatgaagac agacctgtgg gggaagttcc ggatatcggg agtccacacc
     1021 tactccacag ctcccaagca cgccggagga agagtgctgg taaggtttac cgcgaacagc
     1081 gtggtaccgc tggacgaaaa ccaccgtccg ataacgacgc accgcagact gtacggctcg
     1141 ctgaagcagg aggagatgga ttggctgccc tacctgacgc agctctcccg caaccccgga
     1201 gccatgaagt acagcggtat atgggagatg ctgccggacg atcttcgcgg atacctcgaa
     1261 ggaaaggaga agagcggaca aagagatgta ttgcggacgc tcgccctgtt gagcgagcgt
     1321 gacggattcg agcgggccgt gaaaagcgta tcggaagcgt tgaggcgggg gatcgaggac
     1381 cttgacagcc tggtagccct ccacggacac ctgcacggcg aatacccggc cgacagcatg
     1441 cggatcgacg agacgaagct ggggagcctt cccaagcttc cggaggtgcg cttcgcggca
     1501 aaggtttacg acggattcct ttccgccgga ggaagactgc catgttgaaa aacgagatga
     1561 aggacgaact cctcgcatgc tgcaaacggc tcaaactgag ccg
//
LOCUS       DAOTIL010000714         1599 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminobacteriaceae bacterium isolate AMR_MDS_3026
            NODE_91702_length_1599_cov_2.852332, whole genome shotgun sequence.
ACCESSION   DAOTIL010000714 DAOTIL010000000
VERSION     DAOTIL010000714.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868050
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminobacteriaceae bacterium (wastewater metagenome)
  ORGANISM  Aminobacteriaceae bacterium
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae.
REFERENCE   1  (bases 1 to 1599)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1599)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:19:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,224
            CDSs (total)                      :: 3,198
            Genes (coding)                    :: 3,184
            CDSs (with protein)               :: 3,184
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1599
                     /organism="Aminobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_91702_length_1599_cov_2.852332"
                     /isolate="AMR_MDS_3026"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:3034609"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..199
                     /locus_tag="P5201_12465"
     CDS             <1..199
                     /locus_tag="P5201_12465"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRV99402.1"
                     /translation="ALVYCEEAGNAARNRLRSWNWNVAEVPRSLRIRKETGASVSKLL
                     KNYQCFASQRKYVKGQRKCHL"
     gene            complement(246..>1599)
                     /locus_tag="P5201_12470"
     CDS             complement(246..>1599)
                     /locus_tag="P5201_12470"
                     /inference="COORDINATES: protein motif:HMM:NF033594.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ISNCY family transposase"
                     /protein_id="HRV99403.1"
                     /translation="AVILDRVVKGEMTAAEGAARLKMSKRHIFRMKAKIRESDMSALA
                     HGNRCRKPSNALPEKTRALVRDKGMGEYQGASLSHMEELFEKNDGLSISSKSIGRILK
                     EAGATNPHARKSPKKFRRRKRRERFGSLIQVDASPFDWLENGEMLSLHGAIDDATGTV
                     LSLRFEKNECSAGYLHILEETLRRYGVPASLYSDRHSIFFSPKGEKLSEEDIVEGRKA
                     PLTQYGKILDTLGIEHIPARTPQAKGRIERLWGTLQSRLTVEMRICGIKTLEEANFFL
                     RQYTEQHNAQFAEAAADAVSDFLPCPAPESLRLILGYRDERKASSGSEISWKGAKYQL
                     VDEKGKVVLLRRGETVTVVRPADGTLFALREGEKKETSYGLIPSLGGERQKKKGKTSG
                     ESEKKRDEAASRIPPMDHPWRQNWWEKKSGASIIEEELDPMVEGDGDCFGSIRGVRVS
                     "
BASE COUNT          307 a          503 c          387 g          402 t
ORIGIN      
        1 ggcgcttgtg tattgcgagg aggcggggaa tgccgcccgg aaccggcttc gctcctggaa
       61 ctggaacgtt gcggaagttc cgcgaagcct ccgcatacgg aaagaaacag gcgcctcggt
      121 gagtaagttg ttaaaaaatt atcaatgttt tgcgtcgcag agaaagtatg tcaagggaca
      181 gcgaaaatgt cacctctaaa ggtcgaaaac gggacaggga aaatgtcact cccctgtcct
      241 ttttcttatg aaacccgaac accgcgaata ctcccgaaac aatcgccgtc gccctccacc
      301 atcggatcga gttcctcttc gataatcgat gctcccgatt ttttctccca ccagttctgt
      361 ctccatggat gatccatcgg cggtatccgc gatgcagcct catcacgctt cttttcgctc
      421 tccccgctcg tctttccttt ctttttttgt ctctcaccgc caagcgaggg gatcagtcca
      481 tacgacgtct ccttcttctc tccttcacgg agcgcaaaga gcgttccgtc cgcaggccga
      541 acaaccgtaa ctgtttctcc tctgcgaaga agcacaacct tccccttttc gtcgacaagc
      601 tggtatttcg ctcctttcca cgatatctcg gacccggagg acgcctttct ctcatcccga
      661 taccccagga taagccgaag cgactcgggc gcggggcagg ggagaaaatc ggacacggcg
      721 tccgcggccg cctccgcaaa ttgtgcgtta tgctgttccg tgtactgccg cagaaaaaag
      781 ttcgcctcct ccagcgtctt tattccgcag atgcgcatct ctaccgtgag gcggctctgc
      841 agcgttcccc acaaccgctc tatccgtccc ttcgcctgcg gcgtacgggc cgggatgtgt
      901 tctattccca gcgtatccag tattttcccg tactgcgtaa gaggggcttt ccttccttcg
      961 acgatgtcct cctcgctcaa cttttctcct ttgggagaga agaagatgct gtgacgatcc
     1021 gagtagagcg acgcgggaac tccgtatctc ctcaacgtct cctccagaat atggaggtac
     1081 ccggcggaac attcgttctt ctcgaagcgc aacgacagca ccgttccggt ggcgtcgtcg
     1141 atcgccccgt gcagcgagag catctccccg ttttccagcc agtcgaaagg agacgcgtcc
     1201 acctggatca ggctcccgaa acgttcgcgt cgcttccttc tcctgaactt cttcggagac
     1261 ttccttgcgt gaggattcgt cgctccggcc tccttcagaa tacggccgat gctcttggaa
     1321 cttatggaca gaccgtcgtt tttttcgaag agttcctcca tgtgcgacag actcgctcct
     1381 tggtattccc ccattccttt gtcgcgtaca agcgctctcg tcttctcggg aagcgcattc
     1441 gaaggtttcc tgcaccgatt accgtgggcc agagcggaca tatccgattc ccggattttc
     1501 gctttcatac ggaagatgtg tcgtttgctc atcttgagac gggccgctcc ttccgccgcc
     1561 gtcatctctc ctttcaccac tctgtccaga atcaccgct
//
LOCUS       DAOTIL010000715         1595 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminobacteriaceae bacterium isolate AMR_MDS_3026
            NODE_92014_length_1595_cov_3.990260, whole genome shotgun sequence.
ACCESSION   DAOTIL010000715 DAOTIL010000000
VERSION     DAOTIL010000715.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868050
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminobacteriaceae bacterium (wastewater metagenome)
  ORGANISM  Aminobacteriaceae bacterium
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae.
REFERENCE   1  (bases 1 to 1595)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1595)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:19:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,224
            CDSs (total)                      :: 3,198
            Genes (coding)                    :: 3,184
            CDSs (with protein)               :: 3,184
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1595
                     /organism="Aminobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_92014_length_1595_cov_3.990260"
                     /isolate="AMR_MDS_3026"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:3034609"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>1595
                     /locus_tag="P5201_12475"
     CDS             <1..>1595
                     /locus_tag="P5201_12475"
                     /inference="COORDINATES: protein motif:HMM:NF013188.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation:proton antiporter"
                     /protein_id="HRV99404.1"
                     /translation="VGPYTGGITVADVDRIENLAKIGVALLLFSLGLEFSLKQILPIK
                     RIALGGTALQALLTIALGFAIGHFMGWPPLASLWFAVGIISSSTAVILKTLSSRGQLG
                     TLSSRVMLGMSIVQDIAVIPLMVLLMGLDDAVFSVLSIFVPVVKVVLFVSAMLYIGAK
                     FIPLLLKHVARWESRELFLLTVTGIGLGIGYLTYALGLSFAFGAFMAGLVLSESDYGR
                     RALSDLIPVRDLFGLLFFVAIGMLLDPAFLLDHIGVTLGLVTAVAVGRGVILSATVRL
                     FGFRNVIPAAVFFGMIPISEIAFILLQAGLDSGAVPRDVYSFALNTVILSMLLGPPAT
                     ALTAPVYALFKRRRKVDAVQTVNISPDGLSGHVIVAGGGIFARHIGLVLHSMRLPYVV
                     IEPNHALFLEAKKKGLRLLLGDPGQDAVLEAACVEKAKLLVLTLHGALEIQDVVSAVR
                     KRNRTLRILARDVSGEGGEFPDEDREISRIIQPEFEAGLEMARQALIHLDVPGATIHR
                     ELETLRRNVYGPLRENNADYGLLS"
BASE COUNT          245 a          545 c          466 g          339 t
ORIGIN      
        1 gtcggcccct acaccggagg aatcactgtc gcggacgtcg accgcatcga aaatcttgcc
       61 aaaatcggcg tcgcgctcct tcttttctcc ctggggctcg aattctccct caagcagatc
      121 ctcccgatca agcgaatcgc cctcggtggg accgctctcc aggcgttgct gacgatcgcg
      181 ctcgggttcg ccatcgggca tttcatgggg tggccccccc tcgcgtcgct ttggttcgcg
      241 gtagggatca tctcgtcgag caccgcggtc atcctcaaga ccctctcctc ccggggacag
      301 ctcggcacgc tgtcgagcag agtgatgctg ggcatgtcca tcgtgcagga catcgcggtg
      361 attccgctga tggtgcttct aatggggctg gacgacgcag tcttttccgt cctctccatc
      421 ttcgtccccg tcgtcaaggt cgtgctcttc gtctccgcga tgctctacat cggcgcgaag
      481 ttcatcccgc tcctcctgaa gcatgtggcg cggtgggagt cccgggaact ctttcttctc
      541 acggtcaccg gcatcggcct cgggatcggg tacctgacct acgcgctcgg gctctccttc
      601 gccttcggcg ccttcatggc aggccttgtg ctcagcgaat cggactacgg ccgccgcgca
      661 ttgagcgacc ttattcctgt ccgagacctc ttcggactgc tcttcttcgt cgccatcggc
      721 atgctgctcg accccgcctt tctgctggac catatcggcg tcaccctcgg actcgtgact
      781 gccgtcgccg taggccgagg cgtcatcctt tcggccacgg tgaggctgtt cggcttccgc
      841 aacgtcatcc ccgccgcagt cttcttcggc atgatcccga tctccgaaat cgcgttcatc
      901 ctgcttcagg cagggttgga ctccggcgcg gttccgcggg acgtctattc cttcgccctg
      961 aacacggtca tcctctccat gcttctgggt ccgcccgcca ctgcgctgac cgcgcctgtg
     1021 tacgcgctct tcaaaaggcg gcgaaaagta gacgcggtcc agacggtcaa catctctccc
     1081 gacgggttgt ccgggcacgt gatcgttgcc ggcggaggca tcttcgcccg acacatcggg
     1141 ctcgtcctcc acagcatgcg gcttccctac gtcgtcatcg agccgaacca cgcgctcttt
     1201 cttgaggcga aaaagaaggg actgcgactc cttctcggag atcccggaca ggacgcggtt
     1261 ctggaagcgg cctgcgtcga gaaggcaaag ctccttgtcc tgacgctcca tggggctctg
     1321 gagattcagg acgtggtcag cgcggtccgg aagcgcaatc gcactctccg gattctggcc
     1381 cgcgacgtca gcggcgaggg cggggagttc cccgacgagg accgcgaaat ttcgcgcatc
     1441 atccagcccg agttcgaagc ggggcttgag atggcccggc aggcgctcat ccacctcgac
     1501 gtaccgggcg cgacgatcca cagggagctg gagacgctcc ggcggaacgt ctacggcccg
     1561 ctgcgggaga ataacgccga ctacggcctt ctctc
//
LOCUS       DAOTIL010000716         1593 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminobacteriaceae bacterium isolate AMR_MDS_3026
            NODE_92210_length_1593_cov_2.969441, whole genome shotgun sequence.
ACCESSION   DAOTIL010000716 DAOTIL010000000
VERSION     DAOTIL010000716.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868050
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminobacteriaceae bacterium (wastewater metagenome)
  ORGANISM  Aminobacteriaceae bacterium
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae.
REFERENCE   1  (bases 1 to 1593)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1593)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:19:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,224
            CDSs (total)                      :: 3,198
            Genes (coding)                    :: 3,184
            CDSs (with protein)               :: 3,184
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1593
                     /organism="Aminobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_92210_length_1593_cov_2.969441"
                     /isolate="AMR_MDS_3026"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:3034609"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..511)
                     /locus_tag="P5201_12480"
     CDS             complement(<1..511)
                     /locus_tag="P5201_12480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006583735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HRV99405.1"
                     /translation="MKTLSYGRQFIDDDDIDAVVAVLKGDWLTQGPSVKEFEERFAEY
                     VGTSYAVAFANGTAALHGAYFAAGVEAGDEVLTSAMTFAATGNAALYLGGTPVFADID
                     PSTLCLDPKAASKKLSKRTKVIAPVSYAGYPVDIAPFLESAESCGAVVVEDACHALGA
                     ERNGVRVGKE"
     gene            complement(517..999)
                     /gene="tadA"
                     /locus_tag="P5201_12485"
     CDS             complement(517..999)
                     /gene="tadA"
                     /locus_tag="P5201_12485"
                     /EC_number="3.5.4.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008522735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008251 - tRNA-specific adenosine
                     deaminase activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0002100 - tRNA wobble adenosine to inosine
                     editing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA adenosine(34) deaminase TadA"
                     /protein_id="HRV99406.1"
                     /translation="MRLQDEYFMRRALAEAASAAARGDVPVGALVVTGERLVSTGSNR
                     KENDPTAHAEILAMRCAAVSLETWNLGGCTLYVTVEPCPMCAGAIVLARISRLVFGCF
                     DPRAGACGTLYDIVRDGRLNHRCAVRSGVLEGECAAMMTEYFRNRRAVRRKNAVHERS
                     "
     gene            complement(1026..>1593)
                     /locus_tag="P5201_12490"
     CDS             complement(1026..>1593)
                     /locus_tag="P5201_12490"
                     /inference="COORDINATES: protein motif:HMM:NF012993.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="nitrilase-related carbon-nitrogen hydrolase"
                     /protein_id="HRV99407.1"
                     /translation="SCSGDEIAAALSSSLAGEKCMAACAVAEPGAAERRLVLFDSNGL
                     VASLHEGRYTPVETSAGRIGVVFGDEGWNPEPVRCLMLEGAEIVLWYGAFEDERRRSI
                     IEKIARTRASENRVCIVGSFSGPVRESCIIPPSGAVAASALPGESQAVFALVRRSDCR
                     WKTVTPGTDIAAGRHPEAYLPLTETRPF"
BASE COUNT          290 a          557 c          462 g          284 t
ORIGIN      
        1 cttccttgcc gacgcggacc ccgttccgct cagctccgag cgcatggcag gcatcctcga
       61 cgaccaccgc gccgcacgac tccgcggatt cgagaaaggg cgcaatatcc accgggtagc
      121 cggcataact caccggcgcg atcactttcg tgcgcttcga cagcttcttc gacgctgcct
      181 tcgggtcgag gcagagcgtc gagggatcga tgtccgcaaa gacgggcgtg ccgccgagat
      241 agagcgcggc gtttcccgtc gcggcaaagg tcatggcgct ggtgagcacc tcgtccccgg
      301 cttccactcc ggcggcgaag tatgccccgt ggagagcggc cgttccgttc gcgaaggcaa
      361 ccgcgtagct cgttccgaca tactccgcga agcgctcctc gaactccttc acggaaggcc
      421 cctgggtgag ccagtctccc ttgagaacgg ccaccactgc atcgatatca tcgtcgtcaa
      481 tgaactggcg gccgtacgac agcgttttca ttcacatcag ctcctctcat gaacagcgtt
      541 ttttcttcgg acggctcttc tgttcctgaa atactccgtc atcatcgcgg cgcactcccc
      601 ttcgagaact ccggagcgga ctgcgcatct gtggtttaag cggccgtccc ggacaatatc
      661 gtagagagtg ccgcacgctc cggcccgggg atcgaaacag ccgaagacca gcctggaaat
      721 tctcgccagg acgatggcgc ccgcgcacat gggacacggc tccaccgtga cgtacaacgt
      781 acagccgccg agattccacg tctccagtga aactgccgcg cagcgcatgg ccaggatctc
      841 cgcgtgcgca gtcggatcgt tttccttgcg gttgctcccc gtcgagacga gccgctcccc
      901 ggtaacgacc agcgccccca ccggaacgtc tcccctcgcc gccgcggaag cggcctctgc
      961 gagcgcgcgc cgcatgaagt actcgtcctg taaccgcacg gcagcctccg gggtcggcat
     1021 acttctcaga aagggcgcgt ttccgtcaac ggaaggtacg cctcgggatg cctcccggcg
     1081 gcgatgtccg ttcccggcgt cacggtcttc cacctgcagt cggagcgtct gaccagagcg
     1141 aagactgcct ggctctctcc cggaagcgcc gacgcggcga cggctccgga cggcgggatg
     1201 atacagctct cacggaccgg tccagaaaag gaaccgacga tgcacaccct gttttcggaa
     1261 gcccgtgtac gggcgatctt ctcgatgatc gagcgtctgc gttcatcctc gaacgctccg
     1321 taccagagga cgatttccgc cccttccagc atcaggcagc gaacaggttc cgggttccac
     1381 ccctcgtcgc cgaagacgac gcctatcctt cctgcggacg tttccacggg cgtataccgc
     1441 ccctcgtgaa gcgaggcgac taggccgttg ctgtcgaaca gcacaagccg ccgttcagct
     1501 gcccccggct ctgcaactgc gcaggccgcc atgcactttt ctccggcaag cgaggaggaa
     1561 agcgcggcgg cgatctcatc ccccgaacag gaa
//
LOCUS       DAOTIL010000717         1593 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminobacteriaceae bacterium isolate AMR_MDS_3026
            NODE_92230_length_1593_cov_2.301691, whole genome shotgun sequence.
ACCESSION   DAOTIL010000717 DAOTIL010000000
VERSION     DAOTIL010000717.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868050
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminobacteriaceae bacterium (wastewater metagenome)
  ORGANISM  Aminobacteriaceae bacterium
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae.
REFERENCE   1  (bases 1 to 1593)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1593)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:19:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,224
            CDSs (total)                      :: 3,198
            Genes (coding)                    :: 3,184
            CDSs (with protein)               :: 3,184
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1593
                     /organism="Aminobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_92230_length_1593_cov_2.301691"
                     /isolate="AMR_MDS_3026"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:3034609"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(67..>1593)
                     /locus_tag="P5201_12495"
     CDS             complement(67..>1593)
                     /locus_tag="P5201_12495"
                     /EC_number="3.1.1.103"
                     /inference="COORDINATES: protein motif:HMM:NF012371.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase domain-containing protein"
                     /protein_id="HRV99408.1"
                     /translation="MLLDVLRESDLKHAPGAMGIYCNDGFTLAEMIVEKLSGKKFIDF
                     VAERIFEPLGMKDSAASVGETGGNVAVYYDVPTGKKYPLEVVRVHAAGGLSSTAEDLC
                     RFADSFTPEGKHILSEASLAEALKSQPTLFTKHLHDAAMMDAFGWDYAWIPDYREKGV
                     QVLAKSGGTMFYSTNFQVVPAERLVVAVSIAGRADAPGVSRAILDALMQEKGLPFPGK
                     PPLKKPGEPQPIPSELLAFEGAYSDGERAVRFLFDPEKRTLNIHHLLPAPPQGEEAPP
                     PVSLVYSDGVFHNYEKGTSYYFASEGKNVYLIMEKIPGFGMNVPAFQRLEEIKEPKSL
                     SIEMNGVLWMVRNMLPGAQIMNGTLMGWSSTSHDLPGYVFGFSLQKVEDFSFASIAST
                     AFRDQFALQLSRKDGKIRARTGIFVLSPADEAETLADGKNSVTIGPDAENEWRQAGKD
                     MILSFVLPEKGRVIAVDPENDAALLYDSVVDSGEFFAPEGTFLFLAGAAGDVFEIEAR
                     "
BASE COUNT          299 a          505 c          469 g          320 t
ORIGIN      
        1 ggaggggaag cgcagattgt cgcgccccct cctctttgtc tgtccggctg taaaaaatgg
       61 acggctctat ctggcctcta tctcgaacac atcccccgcc gcgcccgcga ggaagagaaa
      121 ggttccctcc ggcgcgaaaa actccccgct gtccacaacg ctgtcgtaaa gcagcgccgc
      181 gtcgttctca gggtcgaccg cgatcacccg tcccttttcg ggcaggacga aggagaggat
      241 catgtccttg ccggcctgcc gccactcgtt ttcggcgtcg ggccctatcg tcacgctgtt
      301 ttttccgtcg gcgagcgttt ccgcctcgtc agccggagag agaacgaata ttccggtcct
      361 cgccctgatt ttgccgtcct tccgggagag ctgaagggcg aactggtcgc ggaaagccgt
      421 tgacgctatg ctcgcgaagc tgaaatcctc gaccttctgc agagagaaac cgaagacgta
      481 cccgggcaga tcgtggctcg tggacgacca gcccatcagc gtcccgttca taatctgcgc
      541 cccgggaagc atgttccgca ccatccagag caccccgttc atctcgatgg aaagagattt
      601 cggctccttg atctcctcca gcctctggaa cgccgggacg ttcatgccga agccgggaat
      661 cttctccatg atcaggtaga cgttttttcc ctcggacgcg aagtagtagg aggttccctt
      721 ctcatagtta tgaaacactc cgtcgctgta gaccaaagag acgggaggag gcgcttcctc
      781 tccctgcgga ggcgcgggaa gcagatggtg gatattcagc gtccgcttct ccgggtcgaa
      841 gaggaaacgg accgccctct cgccgtcgga gtacgctccc tcgaaggcca agagctccga
      901 ggggataggc tgcggctccc cgggtttttt cagaggcggc ttcccgggga aggggagacc
      961 tttctcctgc atgagcgcgt cgagtatcgc acggctaacg ccgggagcgt cggctctgcc
     1021 cgcaatactc acagcgacaa ccaggcgctc ggcaggaaca acctggaagt tggtggagta
     1081 gaacatcgtc ccgccgctct ttgcgagcac ctgcacaccc ttctcgcgat agtccggaat
     1141 ccacgcgtag tcccacccga aggcgtccat cattgcggcg tcgtgcagat gcttcgtgaa
     1201 aagggtcggc tgactcttca gtgcttctgc gagggaggcc tcgctcagga tatgctttcc
     1261 ttcgggcgtg aagctgtccg caaaacggca cagatcctcg gccgtggagg agagtcctcc
     1321 cgccgcatgc acgcggacga cctcaagagg gtattttttg ccggtcggga cgtcgtagta
     1381 cacggcgacg ttgccgccgg tctcgccgac gctcgcggcg ctgtccttca ttcccagcgg
     1441 ctcgaagatg cgctccgcga cgaagtcgat gaacttcttc cccgagagct tctccacgat
     1501 catctccgcg agggtgaagc cgtcgttgca gtagataccc atggcccccg gcgcgtgctt
     1561 cagatccgac tcgcgcagga cgtcgagcaa cat
//
LOCUS       DAOTIL010000718         1591 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminobacteriaceae bacterium isolate AMR_MDS_3026
            NODE_92384_length_1591_cov_2.801432, whole genome shotgun sequence.
ACCESSION   DAOTIL010000718 DAOTIL010000000
VERSION     DAOTIL010000718.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868050
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminobacteriaceae bacterium (wastewater metagenome)
  ORGANISM  Aminobacteriaceae bacterium
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae.
REFERENCE   1  (bases 1 to 1591)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1591)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:19:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,224
            CDSs (total)                      :: 3,198
            Genes (coding)                    :: 3,184
            CDSs (with protein)               :: 3,184
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1591
                     /organism="Aminobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_92384_length_1591_cov_2.801432"
                     /isolate="AMR_MDS_3026"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:3034609"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..563)
                     /locus_tag="P5201_12500"
     CDS             complement(<1..563)
                     /locus_tag="P5201_12500"
                     /inference="COORDINATES: protein motif:HMM:NF013867.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CheR family methyltransferase"
                     /protein_id="HRV99409.1"
                     /translation="MIEKTSYDSPEYDVFKSNVKKLTGLDLNSYKNQIHRRVHMLMQR
                     WSVSSYDIFLKTIKENDQKLREFLDYLTINVSEFFRNPNKWTELQDVVIPRLIQSRGK
                     KQLKLWSAGSATGEEPYSLAILSMEAKLAPQTPVLASDIDQGAIAIAKRGHYLKRQLI
                     NIPKELVGRYFTTNDGGETYLVNSEVKS"
     gene            687..1019
                     /gene="rnpB"
                     /locus_tag="P5201_12505"
     ncRNA           687..1019
                     /ncRNA_class="RNase_P_RNA"
                     /gene="rnpB"
                     /locus_tag="P5201_12505"
                     /product="RNase P RNA component class A"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00010"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00010"
     gene            1163..>1591
                     /gene="mraZ"
                     /locus_tag="P5201_12510"
     CDS             1163..>1591
                     /gene="mraZ"
                     /locus_tag="P5201_12510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013048209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0051302 - regulation of cell division
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="division/cell wall cluster transcriptional
                     repressor MraZ"
                     /protein_id="HRV99410.1"
                     /translation="MLVGTYEHKIDSKARIVLPSKFREELGESVFATIGIDKCVSLYS
                     PEEWKKVMERLQELSFSKSKSRGLLRLMLSSAHELPVDGAGRILLPPVLRDYAEFIQE
                     VVFVGVSDHVEMWDKDKWNDYRKQVLDDLPPLTPAGNLVHG"
BASE COUNT          396 a          378 c          395 g          422 t
ORIGIN      
        1 gatttcacct cggagttcac caggtacgtc tccccgccgt cattagtggt gaagtatcgt
       61 cctacaagtt ctttcgggat gttgataagc tgccgtttaa gataatggcc gcgtttcgct
      121 atcgctattg ccccctggtc gatatcgctt gcgagaacgg gagtctgggg tgcaagtttt
      181 gcctccattg acagaatcgc aagcgaatat ggctcttcgc ctgttgccga acccgcgctc
      241 caaagcttca gttgtttctt tcctcttgat tggatcagcc ttgggattac tacatcctgc
      301 aattccgtcc acttgttcgg attccgaaaa aactccgaaa catttattgt aagataatcg
      361 aggaactcgc gcagtttctg atcattttct ttgatagttt tgaggaaaat gtcgtaggaa
      421 gacacactcc accgttgcat cagcatgtgt acgcggcgat gaatctgatt tttgtaggaa
      481 ttaaggtcaa gccctgtcaa tttttttaca ttactcttga atacatcgta ttctggggaa
      541 tcgtaactcg ttttctctat cattacactc tggctccttc gctcgataat gcacacccgc
      601 ttcgcgtttc gttctctatt gtatcgcatt ttgtgctcga aaaaccattt cgttctcttt
      661 cggaaaagat gatataatgt gcctccggga gtatccttgg tggccgctct tttttgaaga
      721 gaggaaagtc cgggctccac agggcaggac gctgggtaac acccagttgg tgcaaaccaa
      781 aggaaagtgc cacagaaata taccgccctt ttcagggtaa gggtgaaaag gtggggtaag
      841 agcccaccgg gtggaaagtg atttccatgc caggaaaacc ccgtccggag caagaccgaa
      901 taggagggga tgaggttgcc cgccgaccct cgggtacggt cgcgaaaaga acgccgcaag
      961 gtgttcttca gataaatggt cacccacgac agaacccggc ttacaggatg ctccctcttt
     1021 acaatttttc tcaccaaaca aataggcctc aggacccata ttaattgtat tattcaaggg
     1081 tcctgaggcc tacgtatctt ttacaaatga tgatactgtg ggttctagtg ggagagagtg
     1141 tagcaaagtg ggaggcacta ccatgttggt gggaacatat gaacataaaa tcgacagcaa
     1201 ggcaagaatt gtacttccgt cgaagttcag agaagagctc ggggaaagcg tcttcgcaac
     1261 catcggcatt gacaaatgtg tctcccttta ctctcccgaa gagtggaaaa aagtgatgga
     1321 acgtcttcag gaactctctt tctcgaaaag caagagcaga ggattgctcc gtcttatgct
     1381 ttcgagtgct catgagcttc ccgtcgacgg cgccggcaga atactgctcc ctccggtact
     1441 acgcgattat gccgagttca tccaggaggt agtgttcgta ggcgtcagtg atcatgtaga
     1501 gatgtgggac aaagacaaat ggaacgacta tagaaaacag gtccttgacg atctccctcc
     1561 gctgactccc gcgggcaatc ttgtgcacgg t
//
LOCUS       DAOTIL010000719         1590 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminobacteriaceae bacterium isolate AMR_MDS_3026
            NODE_92450_length_1590_cov_3.575244, whole genome shotgun sequence.
ACCESSION   DAOTIL010000719 DAOTIL010000000
VERSION     DAOTIL010000719.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868050
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminobacteriaceae bacterium (wastewater metagenome)
  ORGANISM  Aminobacteriaceae bacterium
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae.
REFERENCE   1  (bases 1 to 1590)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1590)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:19:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,224
            CDSs (total)                      :: 3,198
            Genes (coding)                    :: 3,184
            CDSs (with protein)               :: 3,184
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1590
                     /organism="Aminobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_92450_length_1590_cov_3.575244"
                     /isolate="AMR_MDS_3026"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:3034609"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..954
                     /locus_tag="P5201_12515"
     CDS             <1..954
                     /locus_tag="P5201_12515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005659744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HRV99411.1"
                     /translation="PSGCGKTTLLRMLAGFEDPTSGNIFFGERRMNDIAPNKRNATMV
                     FQSYAIFPHLNVRDNIAFGLKLKDLSKKEIRERTERVVELVGLEGLEERQPSQLSGGQ
                     QQRVALARAVVMEPSVLLFDEPLSNLDAKLREQMRIDIRKLQQRLGITSIYVTHDQIE
                     AMSISDRVVVMNRGRIEQMGSPRELYARPINRFVASFIGKAAFLPAEKDASGAWTLLG
                     KPWRPEAGEELTAGAYEAMIRPEAVRLSPLEDSGRNAIAGTVLRATYLGSVMEYEVEL
                     PGTEPVTVHIPNPFERDPLPPGAEAELRFLPEGLHFLPAEA"
     gene            1300..>1590
                     /locus_tag="P5201_12520"
     CDS             1300..>1590
                     /locus_tag="P5201_12520"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRV99412.1"
                     /translation="MPAAPAVDVSTAFDTGTLPAPSPSALIVGTEFARVNWTDDYVEA
                     SGQAVAPTGKEKTAQGKLLARRGAIVDLQRNLLEAIQGVRVDAKTTMTDFMAN"
BASE COUNT          332 a          492 c          474 g          292 t
ORIGIN      
        1 ccctcgggat gcggcaaaac aacgctcctc cggatgctcg cgggcttcga ggatcctacc
       61 tcggggaaca tcttcttcgg ggagcggcgg atgaacgaca tcgcgccgaa caagcggaac
      121 gcgacgatgg tcttccagtc gtacgccatc ttcccgcacc tcaacgtacg cgacaacatc
      181 gccttcggac tgaaactcaa agatttgagc aagaaggaga tccgcgagcg gaccgaacgc
      241 gtcgtggagc tcgtcgggct ggaggggctg gaggaacgcc agccgagcca gctctcggga
      301 ggacagcagc agcgggtggc gctggcgcgc gccgtcgtga tggagccgtc cgtcctcctc
      361 ttcgacgaac cgctctcgaa tctcgacgcg aagctccgcg aacagatgcg catcgacatc
      421 aggaagttac agcagcgtct cggcatcacg agcatctacg tgacgcacga ccagatcgag
      481 gcaatgagca tctccgaccg cgtcgtcgtg atgaaccggg ggcgcatcga acagatggga
      541 agcccgcggg agctgtacgc acgccctatc aaccgctttg tcgcctcctt catcggcaaa
      601 gcggcctttc ttccggccga aaaagacgct tccggcgcgt ggacgcttct ggggaaaccg
      661 tggcgccccg aagccggaga agaactgacg gccggcgcct acgaggcgat gatccgcccc
      721 gaggccgtgc gcctctcccc tctcgaagac tccggcagga acgcgatagc cgggaccgtg
      781 ctccgcgcca cctatctggg gagcgtgatg gagtacgagg tggagctgcc cgggaccgaa
      841 ccggttacgg tgcacattcc gaacccgttc gagcgcgacc cgctcccccc cggagcggag
      901 gcggagctgc gttttctccc cgaggggctg cactttcttc ctgcggaagc gtagcgccag
      961 aaaaacctcc tgcgagaaat tacgggctga agcgcttctt aaggaaggcg cttcagcctt
     1021 ttttcctatt aaagcaacat cgcgcttcgg ttgaaaattc gaagtaaata gagtatcata
     1081 gtgtacgagg aagacgcgag aatggtatac attttctttt ctctccgctt ttaagcctca
     1141 cccaatcttc ctcgaaaaaa tagagaggag gagtatgcga tgtctcaaaa acgcttcgtt
     1201 tcttgtcttc tgttcctgtt gctcttagga gttccggcgt ttgccacgga ggaggtattc
     1261 cttccaccga tcgaagacct gcctgaggca ggagattccg tgccggccgc gccggcggtc
     1321 gacgtttcca ctgcattcga tactggaacg ctccccgctc catccccgtc cgcgcttatc
     1381 gtcggcactg aattcgcccg cgtcaactgg accgacgatt acgtcgaggc ctcgggacag
     1441 gccgtcgccc cgaccggcaa ggaaaagacc gcgcagggaa aactgcttgc gcgcaggggg
     1501 gccatcgtcg acctccagcg caatcttctt gaggcaatcc agggcgtgcg cgtggacgcg
     1561 aagacgacaa tgaccgactt catggcgaac
//
LOCUS       DAOTIL010000720         1586 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminobacteriaceae bacterium isolate AMR_MDS_3026
            NODE_92790_length_1586_cov_3.548008, whole genome shotgun sequence.
ACCESSION   DAOTIL010000720 DAOTIL010000000
VERSION     DAOTIL010000720.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868050
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminobacteriaceae bacterium (wastewater metagenome)
  ORGANISM  Aminobacteriaceae bacterium
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae.
REFERENCE   1  (bases 1 to 1586)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1586)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:19:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,224
            CDSs (total)                      :: 3,198
            Genes (coding)                    :: 3,184
            CDSs (with protein)               :: 3,184
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1586
                     /organism="Aminobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_92790_length_1586_cov_3.548008"
                     /isolate="AMR_MDS_3026"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:3034609"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1586)
                     /locus_tag="P5201_12525"
     CDS             complement(<1..>1586)
                     /locus_tag="P5201_12525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014163396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HRV99413.1"
                     /translation="PACIGRVCPAPCEKKCRRNFVDDAPVSIREIKRFVGDWAIRNEK
                     MGFIPEIEENGKRIAIVGGGPAGISAAYYLRLKGYAPVIFEKEMLLGGMMRYGIPDYR
                     LPQQVLQAEIDWLLAHGVEVRTGMTLGENITLEALRKEFDGVILAMGCWKSSPMRVPG
                     EDLEGVLGGINFLYDVKTNPSVTIGRRVAVVGGGNTAMDACRSARRLGAEQVSVLYRR
                     SREEMPADNIEIEEATEEGVDFIYLAAPKAIEGNGKVERILCERMQLGEPDASGRRSP
                     VPTGETFILEVDTVIAAVGQGIDFSTVPAELHDGRKMKVGKDYDTPLPGVFVCGDQQT
                     GPKIAIEAIGNGHWAADSLDHYLTHGTPKKPFFYDIVQTDLGPEDFADVERTTQEHVA
                     HVSGETRLAAPFKEYSKGLTEEQTLRDAKRCMECGCPDVFECKLRKFATTHEVQPEKL
                     AGAHIEKFEDANQYYVRNMDKCILCAKCVRACDEISGFHAIDFAKRGFESVLTPHFFN
                     DMEHSDCTFCGLCTQVCPVG"
BASE COUNT          265 a          475 c          489 g          357 t
ORIGIN      
        1 cccgaccggg cagacctggg tgcagagacc gcagaaagtg cagtcggagt gttccatgtc
       61 gttgaagaag tgcggtgtga gtacggactc gaagccgcgc ttcgcaaagt cgatagcgtg
      121 gaaaccggag atttcgtcgc atgcgcggac gcacttcgca cagagaatgc acttgtccat
      181 gttccgtacg tagtactggt tggcgtcctc gaacttctcg atgtgcgcgc ccgccagctt
      241 ctcgggctgt acttcatgcg tagtcgcgaa cttgcggagc ttgcattcga agacgtcggg
      301 gcagccgcac tccatgcagc gcttcgcgtc gcggagggtc tgttcttcgg tcagcccttt
      361 actgtactcc ttgaaggggg cggccagccg tgtttctcct gatacgtgcg cgacgtgctc
      421 ctgggttgtg cgttcgacgt cggcgaagtc ctcggggccg aggtccgtct ggacgatatc
      481 gtagaagaac ggtttcttgg gcgttccgtg cgtcaggtag tggtccagcg aatccgccgc
      541 ccaatgtccg ttgccgatcg cctcgatggc gatcttgggt cccgtctgct ggtcgccgca
      601 gacgaagacg ccgggaagcg gcgtatcata gtctttgccg accttcattt ttctgccgtc
      661 gtgcagctct gccggaacag tggagaagtc gattccctgg cctactgccg cgatgacggt
      721 gtcgacctcg agaataaagg tctcgcccgt gggaacgggg ctccgtctgc cggaggcgtc
      781 gggttcgccg agttgcatgc gttcgcagag aattcgctcg acctttccgt ttccttcgat
      841 tgccttcggc gcggcgagat agatgaagtc gaccccttct tcagtagcct cttctatttc
      901 tatgttgtcg gccggcatct cctcgcggga gcgccggtag agcacggaga cctgctccgc
      961 gccaaggcgg cgggccgaac ggcatgcgtc catcgcggtg tttccgccgc cgacgaccgc
     1021 gactcttctg ccgattgtga cggagggatt tgtcttcaca tcgtacagga agttgatgcc
     1081 tccgagtacg ccttcaagat cctctcccgg aacgcgcatg ggggaggact tccagcaccc
     1141 catggcgagg atcacgccgt cgaactcctt ccggagcgct tcgagtgtga tattttcgcc
     1201 gagggtcatc cctgtgcgca cctccacgcc gtgcgcgagc agccagtcga tctccgcctg
     1261 gaggacctgc tgcggcaggc ggtagtcggg gatcccgtac cgcatcatac cgccgaggag
     1321 catctccttt tcgaagatga ccggggcgta ccccttcagc cgaagatagt aagcggcgga
     1381 aataccggcg gggcctccgc ccacgatagc gattctcttt ccgttttctt cgatctcggg
     1441 aatgaatccc atcttctcgt ttctgatcgc ccagtcgccg acgaatcgtt tgatttcccg
     1501 tattgagaca ggcgcgtcgt cgacaaagtt tctacggcat ttcttctcgc agggggcggg
     1561 gcagaccctg ccgatgcacg caggga
//
LOCUS       DAOTIL010000721         1585 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminobacteriaceae bacterium isolate AMR_MDS_3026
            NODE_92875_length_1585_cov_3.471242, whole genome shotgun sequence.
ACCESSION   DAOTIL010000721 DAOTIL010000000
VERSION     DAOTIL010000721.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868050
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminobacteriaceae bacterium (wastewater metagenome)
  ORGANISM  Aminobacteriaceae bacterium
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae.
REFERENCE   1  (bases 1 to 1585)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1585)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:19:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,224
            CDSs (total)                      :: 3,198
            Genes (coding)                    :: 3,184
            CDSs (with protein)               :: 3,184
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1585
                     /organism="Aminobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_92875_length_1585_cov_3.471242"
                     /isolate="AMR_MDS_3026"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:3034609"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            223..1188
                     /locus_tag="P5201_12530"
     CDS             223..1188
                     /locus_tag="P5201_12530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009165886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HRV99414.1"
                     /translation="MFRFIVKRLFQLVLVLFAVSLIVFLFTSVMGNPVYLMVKENAPA
                     ADIQAVTAALGLDKPLPVQYGIFIKNALQGNFGKSYMNHLPALGLIVERFPATLEIVV
                     VALLLSACIGIPCGVISGAYPKSRFSQVIMAFSIAGISMPSFWIGMVLIYYFSLHLGV
                     LPVSGRGAVGTIMGFKTSLATLDGWQHIILPSVTLALGTLATIIRLTRSGMQENMKQD
                     YVRFAKSKGVPRRTVLFGHALKNTLIPVVTIFGMQMGTLIAFTTITETIFAWPGMGKL
                     LIDSINSLDRPIIAAYILFVAVMFVFINFIVDILYVFIDPRIDLQ"
     gene            1205..>1585
                     /locus_tag="P5201_12535"
     CDS             1205..>1585
                     /locus_tag="P5201_12535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015555935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HRV99415.1"
                     /translation="MTNTARSGRWRAFLDSEFFHNYRRSPSAVIGSILVLFVLLVAIF
                     GPLWTVQNPYNIASLELRDAYKPPAWIEDGEARFLLGTDQQGRDMLSAIVYGSRVSLF
                     IGVAGTLLASAIGIDEKSVRDMQPL"
BASE COUNT          286 a          508 c          443 g          348 t
ORIGIN      
        1 acttcgaggt ggacctgtac gcccacggca agaaggtcac ctacacgccc cgcaagtaca
       61 agtatgtctt cgcgtgggac gtctccttca acgactaaga cgaatgtgcg acgaggttcc
      121 cggcgcgccc tatccggcgc cgggaatttt tgaaaagagc ggcggacctt gctctgacgg
      181 agggtccgcc gtattctgta ccataggtat ggaggtggaa ccgtgtttcg tttcatcgtc
      241 aaaagattgt ttcagctcgt cctcgtgctc ttcgcagtct cgctcatcgt ctttctcttc
      301 accagcgtca tggggaatcc ggtctatctc atggtcaagg agaacgctcc cgccgcggat
      361 atccaggcgg tcacggcggc cctcggactg gacaagccgc tccccgtgca gtacggcata
      421 tttatcaaga acgcgcttca ggggaacttc ggcaagtcgt acatgaacca ccttcccgcc
      481 ctcggcctga tcgtcgaacg cttcccggcc acgctcgaaa tcgtcgtcgt cgcgctgctt
      541 ctgtcggcgt gcatagggat tccctgcggc gtcatatcgg gcgcttatcc gaagagccgc
      601 ttcagccagg tcatcatggc cttttcgatc gcgggcatct ccatgccttc attctggatc
      661 ggcatggtgt tgatctacta tttcagcctt caccttggcg tcctcccggt ttcgggacga
      721 ggcgccgtag gaacgatcat ggggttcaag accagcctcg ccacgctcga cggctggcag
      781 catatcatcc tcccgtcggt cacgctggcg ctggggacgc tcgccaccat catccgcctg
      841 acgcggtccg ggatgcagga gaacatgaag caggactacg tgcgcttcgc caagtccaag
      901 ggcgtgccgc gccgcaccgt gcttttcggt cacgcgctga agaacacgct catccccgtg
      961 gtcacaatct tcggcatgca gatgggaacc ctcatcgctt tcaccaccat cacggagacc
     1021 atcttcgcct ggcccggcat ggggaagctg ctgatcgact cgatcaacag tctcgaccgg
     1081 ccgatcatcg cggcgtacat cctcttcgtg gcggtgatgt tcgtcttcat caacttcatc
     1141 gtggatattc tctacgtctt catcgatccc aggatcgatc ttcagtgaaa gggggcggcg
     1201 tataatgacg aataccgcgc gctccggccg ctggagagcc tttctggatt cggagttctt
     1261 ccataactac aggcgctctc cctccgccgt catcggctcc attcttgtcc tcttcgtcct
     1321 tctggtcgcc atattcggcc ctctgtggac ggtgcagaat ccgtacaaca tcgcgtccct
     1381 cgaacttcgg gacgcctaca agccccccgc gtggatcgag gacggggagg cgcggttcct
     1441 cctgggaacc gaccagcagg ggcgcgacat gctcagcgcc atcgtctacg gcagcagagt
     1501 ctccctcttc atcggcgtgg ccggaacgct gctggcaagc gcgatcggca tcgacgagaa
     1561 gagcgtgcgg gacatgcagc ccctg
//
LOCUS       DAOTIL010000722         1584 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminobacteriaceae bacterium isolate AMR_MDS_3026
            NODE_92954_length_1584_cov_3.930020, whole genome shotgun sequence.
ACCESSION   DAOTIL010000722 DAOTIL010000000
VERSION     DAOTIL010000722.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868050
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminobacteriaceae bacterium (wastewater metagenome)
  ORGANISM  Aminobacteriaceae bacterium
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae.
REFERENCE   1  (bases 1 to 1584)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1584)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:19:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,224
            CDSs (total)                      :: 3,198
            Genes (coding)                    :: 3,184
            CDSs (with protein)               :: 3,184
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1584
                     /organism="Aminobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_92954_length_1584_cov_3.930020"
                     /isolate="AMR_MDS_3026"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:3034609"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            106..1077
                     /locus_tag="P5201_12540"
     CDS             106..1077
                     /locus_tag="P5201_12540"
                     /inference="COORDINATES: protein motif:HMM:NF014476.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine cyclodeaminase family protein"
                     /protein_id="HRV99416.1"
                     /translation="MYYLNAESMISSFSFSGVMDAVANAMIQIETSPNSLIAPLRNSV
                     SLEKNTLMMMPCLSEKEWGLKVLTLFPDNPVRLKPFINGLFLLFDGSDGSPSALLDGR
                     TLTAIRTGGVGGLAVRTLASTHATRLGIVGAGEQSYWQVRFACSVRPFSQVILYDTIE
                     SKAEFCAERIRKVLPELEIEITKDTTFLLKHSDVVITATNTTTPVFEDSPMYFEGKTF
                     VGIGSYRPDMREYPDALFRAISNIFVDTEHAFDETGDLIEPLQSGVVLKKNIRSMGAF
                     LSREKADFTAESGAFFKSVGYAAFDLFVARFLFQRGVENGKAIDLGI"
     gene            1120..>1584
                     /locus_tag="P5201_12545"
     CDS             1120..>1584
                     /locus_tag="P5201_12545"
                     /inference="COORDINATES: protein motif:HMM:NF025559.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-2-amino-thiazoline-4-carboxylic acid
                     hydrolase"
                     /protein_id="HRV99417.1"
                     /translation="MREEDETLLRKSVDAMARALALYHGIFAREAVGMLGQEKGTALV
                     ENVVRQFGLERGRRVREQALAAGDALDLKGMNEHYDLPLAYAWDALKQENGSDVSFCP
                     MADEWKRNGMVEEGKLYCAVDYAIAEGFSPDLHFSRSSSLMHEDSCCCHRYSN"
BASE COUNT          374 a          327 c          407 g          476 t
ORIGIN      
        1 ttgcttaagg ggtgttttga atatatcctg tactttatat tgccatttcg cttcttttta
       61 tattcatttt tccttctttt attctaacaa agggggttgt atgttatgta ttacttgaat
      121 gcggaaagca tgatttcttc tttttctttc tcaggtgtaa tggacgctgt tgccaatgcc
      181 atgattcaga ttgaaacatc cccaaacagc cttatcgctc ctctccgtaa ttctgtctct
      241 cttgagaaaa atacactgat gatgatgccc tgtctttctg agaaagagtg gggactgaag
      301 gtgttgacac tctttcccga taatccggta cgactcaaac catttattaa cggtcttttt
      361 cttcttttcg acggttcgga tggcagtccc tccgctcttc ttgacggaag gactttaacc
      421 gcgattcgga ctggaggggt aggaggcttg gctgttcgca ccctcgcttc tactcatgca
      481 acccgcttgg gaatcgttgg cgctggggaa caaagctatt ggcaggtccg ctttgcgtgt
      541 tccgttcgtc ctttttccca agtgattctt tatgatacaa tcgaatcgaa agccgagttt
      601 tgtgccgaac gtatacgtaa agtcctcccc gaattggaga tcgagattac gaaagataca
      661 acatttttgt taaagcactc ggatgtggta ataactgcga ccaataccac gactcctgtt
      721 tttgaggata gtccgatgta tttcgaaggg aaaacttttg tcggtatagg ttcgtaccgt
      781 ccggatatga gagagtatcc ggacgcgctt ttcagagcaa tttcaaacat ttttgtagat
      841 actgagcatg ctttcgacga gactggcgac cttattgaac ctttacagtc aggtgttgtt
      901 ttaaagaaaa atatccgttc gatgggagct tttctatccc gtgaaaaagc cgactttacc
      961 gctgagtccg gagcgttttt caaatccgtc ggctatgctg ctttcgatct tttcgtagcg
     1021 cggtttcttt tccagcgggg cgtagagaat ggaaaggcta tagacttggg aatataagca
     1081 ggcatccggg gaaacactcg gaaagaaggt ggggaggata tgagagaaga agatgaaact
     1141 ctattgagaa agagcgtcga tgcaatggcc cgtgcattgg ctctctacca tggcattttt
     1201 gcccgtgagg cggtagggat gctggggcag gagaaaggga ccgctcttgt tgagaatgtt
     1261 gtgcgtcagt tcggtcttga acgtgggcgg cgtgtcaggg aacaagcgct cgctgcgggt
     1321 gatgcgctgg atttgaaagg aatgaatgaa cactacgatt tgcctcttgc gtatgcatgg
     1381 gacgcattga agcaggaaaa tggaagcgat gtctcttttt gtccgatggc cgacgaatgg
     1441 aagcgaaacg gtatggtgga ggaaggaaaa ttgtattgcg ctgtagatta tgccattgcc
     1501 gaaggcttct ctcctgattt gcatttttca agatcctcca gtttgatgca cgaagattcc
     1561 tgttgttgcc atcgctactc caac
//
LOCUS       DAOTIL010000723         1584 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminobacteriaceae bacterium isolate AMR_MDS_3026
            NODE_92974_length_1584_cov_2.858731, whole genome shotgun sequence.
ACCESSION   DAOTIL010000723 DAOTIL010000000
VERSION     DAOTIL010000723.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868050
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminobacteriaceae bacterium (wastewater metagenome)
  ORGANISM  Aminobacteriaceae bacterium
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae.
REFERENCE   1  (bases 1 to 1584)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1584)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:19:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,224
            CDSs (total)                      :: 3,198
            Genes (coding)                    :: 3,184
            CDSs (with protein)               :: 3,184
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1584
                     /organism="Aminobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_92974_length_1584_cov_2.858731"
                     /isolate="AMR_MDS_3026"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:3034609"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..288)
                     /locus_tag="P5201_12550"
     CDS             complement(<1..288)
                     /locus_tag="P5201_12550"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF025054.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HRV99418.1"
                     /translation="MNTTYRFMRQGEEERVCALVERVFNEFVAPDYSPEGVEEFFKFA
                     APVALRNRNGEHHMVILAEEETELAGVLEARDCNHISLLFVERKFHRKGIAK"
     gene            542..895
                     /locus_tag="P5201_12555"
     CDS             542..895
                     /locus_tag="P5201_12555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009200445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HRV99419.1"
                     /translation="MGKEELNLKKAGILKGLAHPVRLRIVEALSKKDMCVCEIAELFN
                     FDRTTISKHLALMKSIGILGDRKEGLNVYYSLRMRCLASLLVCVERVVSGDEPEAMMW
                     ACGCGALENTNDQEG"
     gene            899..1141
                     /locus_tag="P5201_12560"
     CDS             899..1141
                     /locus_tag="P5201_12560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013047475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015036 - disulfide oxidoreductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HRV99420.1"
                     /translation="MKIQILGTGCPKCKKLGELADQAAKEMGLEFEIEKVTGIADIME
                     FGVMTTPALAVDGKVLSAGHVPSYEKVKELLLKAKG"
     gene            1243..>1584
                     /locus_tag="P5201_12565"
     CDS             1243..>1584
                     /locus_tag="P5201_12565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006584295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="permease"
                     /protein_id="HRV99421.1"
                     /translation="MSDRKKFFWIAAFFLTFYFIPEGNERVLAGLAESVAMMADYARE
                     HVLLCLIPAFFIAGAISVFVSQQSVMKYLGAKAKKWIAYSVASVSGTVLAVCSCTVLP
                     LFAGIYTRGAGL"
BASE COUNT          362 a          393 c          476 g          353 t
ORIGIN      
        1 ttttgctatg cccttacggt gaaacttccg ctccacgaag agcagcgaga tgtggttgca
       61 gtcgcgcgct tcaagtaccc cggcaagctc cgtttcctct tcggcaagaa taaccatgtg
      121 gtgttcacca ttccggtttc gcaacgccac cggagccgcg aatttgaaga actcctcgac
      181 gccctccggg ctgtagtccg gagcaacgaa ttcattgaag accctctcga cgagagcgca
      241 gacgcgctcc tcctccccct ggcgcatgaa acggtacgta gtattcatcg aatgacctcg
      301 ttgtgttgaa aaaagattgc tcgaaaattc tccgctcggg aaagagaagc aggggagaaa
      361 gtcgaccagt gcattgagct gaatgataga aaatttaacg ctttttgtca atagactcga
      421 gaaaaaggaa tatatgatcc acggcgccga tggacgccat tcgttctgaa aggagcggag
      481 gaaagcatcg cttgacgggt gttttttatg tggtattatt gccacataag gaggcggtgt
      541 cgtgggaaaa gaggaattga atctcaaaaa ggccggcata ttgaaggggc ttgcccatcc
      601 cgtccgtctc cgcatcgtag aagctttgtc gaagaaggat atgtgcgtgt gtgagatagc
      661 cgaactgttc aatttcgacc ggacgacgat cagcaagcat cttgcgctga tgaaaagcat
      721 cggcatcctt ggggaccgga aagaggggct gaacgtttac tacagtttgc ggatgcgctg
      781 tctggcatcg ctcctggtct gcgtggagcg ggttgtttcc ggagatgagc ccgaggcgat
      841 gatgtgggcc tgcggatgcg gggcattgga gaacacgaac gatcaggagg ggtaagatat
      901 gaaaattcag attctgggca caggatgccc aaagtgcaag aagctcggcg aactcgccga
      961 ccaggctgcg aaggaaatgg gcctggagtt tgaaatcgag aaagtgacgg gcatcgcaga
     1021 tatcatggag ttcggcgtca tgaccacccc cgcgctcgcc gtggacggca aggtgctctc
     1081 cgccgggcat gttccttcct acgagaaggt gaaggagctg cttctgaagg cgaaggggta
     1141 agccgataca ccatgccccc gctcccggcg ggggtttccc cttgttcttt aagtgaataa
     1201 aacgccacat aatggcgata ctgtatgaaa tggagagaga caatgagcga tcgtaaaaag
     1261 tttttttgga tagccgcgtt ttttctgacg ttctacttca tccccgaggg gaacgagcgc
     1321 gttcttgcag gactcgcgga gtccgtggcc atgatggccg actacgcccg ggagcacgtg
     1381 ctgctctgcc tgattcccgc gttcttcatc gccggggcca tctcagtttt cgtgagtcaa
     1441 cagtcggtga tgaagtacct gggtgcaaag gcgaagaagt ggatcgccta ctccgttgca
     1501 tcggtttccg gcacggtgct ggcggtctgc tcctgcacgg tgctgcctct cttcgcgggg
     1561 atctacaccc ggggcgcggg gctc
//
LOCUS       DAOTIL010000724         1581 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminobacteriaceae bacterium isolate AMR_MDS_3026
            NODE_93228_length_1581_cov_2.689384, whole genome shotgun sequence.
ACCESSION   DAOTIL010000724 DAOTIL010000000
VERSION     DAOTIL010000724.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868050
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminobacteriaceae bacterium (wastewater metagenome)
  ORGANISM  Aminobacteriaceae bacterium
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae.
REFERENCE   1  (bases 1 to 1581)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1581)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:19:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,224
            CDSs (total)                      :: 3,198
            Genes (coding)                    :: 3,184
            CDSs (with protein)               :: 3,184
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1581
                     /organism="Aminobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_93228_length_1581_cov_2.689384"
                     /isolate="AMR_MDS_3026"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:3034609"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            6..491
                     /locus_tag="P5201_12570"
     CDS             6..491
                     /locus_tag="P5201_12570"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009202278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HRV99422.1"
                     /translation="MKEGRHVSNDLAERCLKTAAATGAFTADELDVLEDVLVEWSLHP
                     GRDYILLTEWIDGDIAGFLIYGPTPMTRFAYDLYWIAVAPHHQKKGLGGTLEEKMCSA
                     LLEQSDSAVIRVETAGRDDYLGQRYFYLARHYGECGRIPDFYSEGDDLVLYCKKLTKK
                     R"
     gene            607..>1581
                     /locus_tag="P5201_12575"
     CDS             607..>1581
                     /locus_tag="P5201_12575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013047598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="KamA family radical SAM protein"
                     /protein_id="HRV99423.1"
                     /translation="MPCDSSGKKFLEALRDSAGEDRWNDWRWQMQSRITTADELGRIL
                     TLSRGEGATLRKSLNSLRMAITPYYASLIDPDDPECPIRKQAVPTLKETLIAPTDLLD
                     PLHEDVDSPVPGLTHRYPDRCILLVTDQCGMYCRHCTRRRFAGQTDTARSSEQIRACI
                     DYIARTPEIRDVLITGGDPLTLSDEALDSILSEIRAIPTVEIIRIGTRTPAVLPMRVT
                     ERLCAVLKKYHPLWINLHFNHPRELTPEAARACALLADAGIPLGNQSVLLKGVNDCPY
                     IFRDLNQKLMKMRVRPYYIYQCDLSRGIEHFRTSIGKGLEIMEYLRGHT"
BASE COUNT          347 a          483 c          455 g          296 t
ORIGIN      
        1 agaagatgaa ggagggacga cacgtgagta acgatctcgc cgagcgctgc ctgaagaccg
       61 cagcggcaac aggagccttc accgccgacg aactggacgt tctcgaggac gtgctggtcg
      121 agtggtcgct ccacccgggg cgcgattata tcctgctcac ggagtggatc gacggagaca
      181 ttgccgggtt cctgatctac ggtcctaccc cgatgacacg tttcgcctat gacctctact
      241 ggatcgccgt agctcctcac catcagaaaa aaggcctcgg aggcacacta gaggagaaga
      301 tgtgctcagc ccttctggag cagtcggaca gcgcggttat ccgtgtcgaa acggcgggaa
      361 gggacgatta cctgggacag cgctacttct atcttgcacg gcattacgga gaatgcggcc
      421 gcattcccga cttttattcg gaaggtgacg atcttgtcct ctactgcaaa aaactcacga
      481 aaaagcgtta atatcgtcaa cacgccggat gcctcgccgg aggaaacaaa acctccgtcg
      541 aaaggccgtc gcatcaggga tgcttttccc cagctttcgc ttctcgccgg gacaaacgct
      601 cccttgattc cgtgcgattc gagcgggaag aaattcctcg aagcgctcag agattctgca
      661 ggggaagatc gatggaacga ctggcgctgg cagatgcagt cgcgaatcac gacggccgat
      721 gaactgggtc gtattctcac gctatcgcgc ggagagggcg cgacgctgcg gaagagcctg
      781 aactcgctcc gtatggcgat taccccgtac tatgcgtcgc tgatcgatcc ggatgacccg
      841 gaatgcccca tcaggaaaca ggccgtgccg acgcttaagg agacgctcat cgcgccgacc
      901 gacttgcttg acccgctgca cgaggacgtg gattctccgg taccggggct gacgcaccgc
      961 taccccgaca ggtgtattct gctggtcacg gaccagtgcg gcatgtactg caggcactgc
     1021 actagacggc gttttgcagg gcagaccgat acggcccgtt cctccgaaca gatacgggcg
     1081 tgcatcgact acatagcacg tacgcccgag attcgcgacg tgctcatcac aggcggcgat
     1141 cccctcacgc tctccgacga ggctctggat tccatcctct ccgagatccg ggcaattcct
     1201 acggtcgaga tcatcaggat aggaacgcga acgccggcgg tgctgccgat gcgagtgaca
     1261 gagcggctgt gcgccgtgct gaaaaaatac caccctctct ggatcaacct gcatttcaac
     1321 catccaaggg agctcacccc cgaggccgcc cgcgcctgcg ctcttctcgc cgacgcaggt
     1381 attcccctgg gcaaccagtc ggtgctcctc aagggcgtga acgactgtcc gtacatcttc
     1441 cgcgacctca accagaagct gatgaagatg cgggtgcgcc cctactacat ctaccagtgc
     1501 gacctgtccc ggggaataga gcacttcaga acctccatcg gcaagggact ggagattatg
     1561 gagtacctgc ggggccatac c
//
LOCUS       DAOTIL010000725         1578 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminobacteriaceae bacterium isolate AMR_MDS_3026
            NODE_93485_length_1578_cov_2.213395, whole genome shotgun sequence.
ACCESSION   DAOTIL010000725 DAOTIL010000000
VERSION     DAOTIL010000725.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868050
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminobacteriaceae bacterium (wastewater metagenome)
  ORGANISM  Aminobacteriaceae bacterium
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae.
REFERENCE   1  (bases 1 to 1578)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1578)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:19:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,224
            CDSs (total)                      :: 3,198
            Genes (coding)                    :: 3,184
            CDSs (with protein)               :: 3,184
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1578
                     /organism="Aminobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_93485_length_1578_cov_2.213395"
                     /isolate="AMR_MDS_3026"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:3034609"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            204..1394
                     /locus_tag="P5201_12580"
     CDS             204..1394
                     /locus_tag="P5201_12580"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRV99424.1"
                     /translation="MERTTAMVISHLEWFSLLRPLEEDVAQSYTLPVEQDAQHASTGV
                     AATLRRRGPNAIARASKEFAETKETLAVSGAQNARTSETLEIKERKNIDAHWKSIIKR
                     FFVRVLRRILPELADDLDTARKVVFLDKELAELTVYIDGPEQRTDVLARVPTKSGRDV
                     WLILHVEIQGPEGGDLPERMFYYNSSLRVTYLKKKSDISDAISFAILTAKRPCGEDEL
                     YLRESYRNRLAYEYPSLKLWELDSVELEASMNPFDWALYAGKCALESGRNDRLKVDYL
                     KKLSEKLDSKGWSHEEKLALFRFMDLLLRPKSPEMRKEFDVYVDRRKKEGKIVYLSMI
                     EENAEKRGEKRGEKRGEKRGEKEKALAVASRMLDAGEPVEKILRYAEISPKELDDLIK
                     TREN"
BASE COUNT          372 a          359 c          524 g          323 t
ORIGIN      
        1 cgaaaagagc tgcttgtatc ctttgtcttg atctcccatg tccgaattat agatgtgaag
       61 aagaggtttg gcaaggcgcg atggagcggg attcgaggat cgggtcggac ttcctgtacg
      121 cagaggcgcg aacgcgcgtc ttcacgtgta cgacccggtg gcggtggaga atgctcggcg
      181 tggagcggcg cggagggtag cgtgtggaga gaactaccgc tatggtgatc tcacatctcg
      241 aatggttttc tctacttcgt ccacttgaag aagatgtcgc tcaaagctat actcttcctg
      301 tggaacaaga cgcacaacat gcttcgacgg gtgttgctgc gaccctgaga cgaagggggc
      361 cgaacgccat agcacgcgct tcgaaggagt tcgcggaaac gaaggagaca ctcgctgttt
      421 ccggtgcaca aaacgcgaga acgagcgaaa cgctcgagat aaaggaacgg aagaatatcg
      481 acgcgcactg gaagtcgatc atcaagaggt tcttcgttcg agttcttcga agaattcttc
      541 cggagctggc cgacgatttg gataccgccc ggaaggtggt cttcctggac aaggaacttg
      601 cggaactgac ggtctacatc gatggtcccg agcagcgcac cgatgtgctc gcccgtgttc
      661 cgacgaagtc cggacgcgac gtttggctga ttctccacgt ggagatacag ggaccggagg
      721 gaggcgacct gccggagcgg atgttctact acaactcctc tctgcgggtg acgtacctca
      781 agaagaagag cgacatttcg gacgcgatct cttttgccat tctgacggcg aagcgcccct
      841 gcggcgagga tgagctgtac cttcgggaga gctaccggaa ccgtttagcg tacgagtacc
      901 cgtcgctgaa gctgtgggag cttgattccg tggagcttga ggcaagtatg aatccgttcg
      961 actgggcttt gtacgccggg aagtgcgcgc ttgagagcgg caggaacgac agactgaagg
     1021 tggactacct gaagaagctg agtgagaaac tggattccaa ggggtggtcg cacgaggaga
     1081 agctggcgct cttccgcttc atggacctgc tgctccgtcc gaagagtccg gagatgagaa
     1141 aagaattcga tgtgtatgtt gaccgtagga agaaggaggg gaaaatcgtg tatttgagta
     1201 tgatcgagga gaatgcggag aagagagggg agaaaagagg ggagaagaga ggcgagaaga
     1261 gaggcgagaa ggagaaggct cttgcggtcg cctccaggat gttggatgcc ggggagcctg
     1321 tggagaagat tcttcgttat gcggagattt cccccaagga gcttgatgac ctgatcaaga
     1381 cgcgggaaaa ttaaacacgc cgctgatctt cgacggggtt tgagtacgcg ggcatcgaaa
     1441 tgtgtcgagg ttgctaaagg tcgacgcttc tcccgggatc ttaccggttg gaaggcaggc
     1501 ggctgaagag gatttcgttc gcacggtggt aggactcgaa ggtgccggtg tccatccaca
     1561 ttccgcggac gaagtcgt
//
LOCUS       DAOTIL010000726         1576 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminobacteriaceae bacterium isolate AMR_MDS_3026
            NODE_93603_length_1576_cov_4.054569, whole genome shotgun sequence.
ACCESSION   DAOTIL010000726 DAOTIL010000000
VERSION     DAOTIL010000726.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868050
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminobacteriaceae bacterium (wastewater metagenome)
  ORGANISM  Aminobacteriaceae bacterium
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae.
REFERENCE   1  (bases 1 to 1576)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1576)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:19:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,224
            CDSs (total)                      :: 3,198
            Genes (coding)                    :: 3,184
            CDSs (with protein)               :: 3,184
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1576
                     /organism="Aminobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_93603_length_1576_cov_4.054569"
                     /isolate="AMR_MDS_3026"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:3034609"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..436
                     /locus_tag="P5201_12585"
     CDS             <1..436
                     /locus_tag="P5201_12585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006301075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DNA translocase FtsK"
                     /protein_id="HRV99425.1"
                     /translation="LLGKGDMLFVSSKYPRPLRLQAPFIEDGKSLEIIEYLGNIFGPP
                     EYVDIEAQGAGESTNNGDTSFAEDPLLEEAVDIVMETGIASASRLQRQLRVGFTRAAR
                     LVDTMEQIGIVGPPEGSKPREILVDDETARSLLTRVLSGEGE"
     gene            438..1148
                     /locus_tag="P5201_12590"
     CDS             438..1148
                     /locus_tag="P5201_12590"
                     /EC_number="2.7.6.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR01378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004788 - thiamine diphosphokinase
                     activity [Evidence IEA];
                     GO_process: GO:0006772 - thiamine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine diphosphokinase"
                     /protein_id="HRV99426.1"
                     /translation="MFSLSLPNGKATFSNAFQEPLLVVAGGRKPAASWLAEIFRSFTT
                     RWCADSGLVYCLEAGILPDRLVGDGDSTPPDKWRRAVEAGVSVTKHPVDKDLTDLQLA
                     LFTAGKELPGKPVLVTGCWGGRFDHLWANIFSALWAAEQNIRILVFADEKECLFLLEG
                     EEALEICVSGRENVVVSLLPLTPECAGVDLQNVRWELKDSLLLQKHPYTVSNRTEGNK
                     NISVRLQKGILGVYCGWL"
     gene            complement(1165..1359)
                     /gene="rpmB"
                     /locus_tag="P5201_12595"
     CDS             complement(1165..1359)
                     /gene="rpmB"
                     /locus_tag="P5201_12595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006583527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L28"
                     /protein_id="HRV99427.1"
                     /translation="MAKVCECCGRGPTTGNAVSHSNRHTRRRWLVNLRSVKADVGGGE
                     SLRMRICTRCLRSGKVKRAV"
     gene            1471..>1576
                     /gene="xseB"
                     /locus_tag="P5201_12600"
     CDS             1471..>1576
                     /gene="xseB"
                     /locus_tag="P5201_12600"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: protein motif:HMM:TIGR01280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009318 - exodeoxyribonuclease VII
                     complex [Evidence IEA];
                     GO_function: GO:0008855 - exodeoxyribonuclease VII
                     activity [Evidence IEA];
                     GO_process: GO:0006308 - DNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII small subunit"
                     /protein_id="HRV99428.1"
                     /translation="MSFSGKMDELEGIVARIEKEALPLEDALALFEKGI"
BASE COUNT          362 a          372 c          459 g          383 t
ORIGIN      
        1 tctcctgggg aagggcgaca tgcttttcgt cagttcaaag tatcctcgcc ccctccgtct
       61 tcaggctccc ttcatcgaag acggcaagag ccttgagatc atagagtacc tgggaaatat
      121 cttcggcccg cctgagtacg tcgatatcga agcgcagggc gctggagaga gcacgaacaa
      181 cggcgatacc tcttttgccg aggaccccct tcttgaagag gccgtcgata tcgttatgga
      241 aacaggtatc gcatcggcaa gcagattgca gcggcagctc cgcgtaggct ttacacgcgc
      301 tgcgcgtctc gtggatacca tggagcagat cgggattgtc ggacctcctg aagggtcaaa
      361 gcctcgtgaa atacttgttg acgacgaaac ggcgaggagc ctcctcacac gggtgctttc
      421 gggagaggga gagtagggtg ttctctctct ctcttcccaa tgggaaggca accttctcaa
      481 acgcttttca agagcctctt ctggtcgttg cagggggaag aaaaccggct gcttcctggt
      541 tagctgagat ttttcgctcg tttacgacgc gctggtgcgc tgattccggc cttgtgtatt
      601 gtcttgaagc gggtattctg ccggacagac ttgtgggaga cggagacagt actcctccgg
      661 ataaatggag gcgtgctgta gaggcgggag tttccgtaac gaaacaccct gtggataagg
      721 atttgacaga tcttcagctg gcgctcttca cggcgggaaa agagctcccc ggaaaaccgg
      781 ttctggtaac cggttgctgg ggagggaggt ttgaccatct ctgggcgaat atattctccg
      841 cgttgtgggc ggccgaacag aacattcgca ttcttgtatt tgcggatgaa aaggagtgtc
      901 tgttcctctt ggagggggaa gaagctctcg aaatttgcgt ttccgggcgt gagaatgtcg
      961 tagtgtcgct gttgccgctt actccggaat gcgccggtgt tgatctccaa aatgttcgtt
     1021 gggagttgaa ggattccctt ctactgcaaa aacaccccta tacagtgagc aacagaaccg
     1081 aggggaacaa aaatatctca gtaaggttgc agaagggaat ccttggtgta tactgcggat
     1141 ggctctgaaa agcaaaagag ggctttacac cgcccgtttc actttgccgg aacggagaca
     1201 gcgtgtgcag attctcatgc gaagagattc gccgccgcca acgtcagcct tgacgctccg
     1261 aagattgaca agccagcgcc tgcgtgtatg ccggttagaa tgacttacgg catttccggt
     1321 ggtaggcccg cgaccgcaac attcacatac ttttgccatt gatttcgccc ccttgctgcg
     1381 attggtttcg ataaaacaga agaattttag cacagagggg tgcatggagc aaattttatt
     1441 tgtagaattc ctcggggagg attggacaaa atgagtttta gcgggaaaat ggatgaactt
     1501 gagggtatcg tagcccgtat tgagaaggaa gctctgcctc ttgaagacgc tctcgcgctt
     1561 tttgaaaaag ggattg
//
LOCUS       DAOTIL010000727         1571 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminobacteriaceae bacterium isolate AMR_MDS_3026
            NODE_94075_length_1571_cov_3.006596, whole genome shotgun sequence.
ACCESSION   DAOTIL010000727 DAOTIL010000000
VERSION     DAOTIL010000727.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868050
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminobacteriaceae bacterium (wastewater metagenome)
  ORGANISM  Aminobacteriaceae bacterium
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae.
REFERENCE   1  (bases 1 to 1571)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1571)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:19:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,224
            CDSs (total)                      :: 3,198
            Genes (coding)                    :: 3,184
            CDSs (with protein)               :: 3,184
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1571
                     /organism="Aminobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_94075_length_1571_cov_3.006596"
                     /isolate="AMR_MDS_3026"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:3034609"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(257..535)
                     /locus_tag="P5201_12605"
     CDS             complement(257..535)
                     /locus_tag="P5201_12605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008657 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) inhibitor activity
                     [Evidence IEA];
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA];
                     GO_process: GO:0030541 - plasmid partitioning [Evidence
                     IEA];
                     GO_process: GO:0051290 - protein heterotetramerization
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HRV99429.1"
                     /translation="MSVRSVEIDAETAKKIQKLDRETQRLILSYIDRHLRNGTDPRAS
                     GKALTGPLRGLWRYRIGDYRLLAEIRDDELIIVAVDIGHRSAIYSRRR"
     gene            complement(532..771)
                     /locus_tag="P5201_12610"
     CDS             complement(532..771)
                     /locus_tag="P5201_12610"
                     /inference="COORDINATES: protein motif:HMM:NF039402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6290 family protein"
                     /protein_id="HRV99430.1"
                     /translation="MATLEKTLTVRLTPEERMAVEEYAKEKNMTIAQLARESLLEKIE
                     DAYDLEVYTAWLKSKRETVRFEDLVKECGFSEEDL"
     gene            complement(1049..>1571)
                     /locus_tag="P5201_12615"
     CDS             complement(1049..>1571)
                     /locus_tag="P5201_12615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008709835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="glutamine synthetase"
                     /protein_id="HRV99431.1"
                     /translation="HGLSKEGLAYTSGLLEHSLTGSLLAFTNPSTNSFRRLVPGYEAP
                     ISATFAKGSRSAAVRIPGYLKKDEVRVEYRTGDASANIHYMLSAMVLAGVDGILRNAD
                     PVANGFSSAEAKQEKVFPLSLNAVLDGLKKDNEWLKPAFPEKLMELWTKRKLEEAEYV
                     YNAPTPQEYELYF"
BASE COUNT          307 a          484 c          436 g          344 t
ORIGIN      
        1 tggagcgctt gctccgcatg gcggggtgtt ctttgaaacg cgagggcgct tcgcatgctc
       61 tgtggatcaa cccgaaaacc ggtgcgatgg aagctgttcc tcggcatacg gagatcaaag
      121 aaccgttggc gaaaaaaact gaagaacctc gaagcgtgag atgccttggt tgagatgcct
      181 tggtcgggat gccggttctc ggattctcga tctccgaaat ggagagcgtc ctccatcgcc
      241 cgcaggagta cgcagcctat ctccttcggg aatagatcgc cgaacgatga cctatatcca
      301 cggcgacgat gatgagttcg tcatcccgta tctcggcaag cagacggtag tctccgatcc
      361 tgtagcgcca tagtccgcgc agcggacccg tgagagcttt cccggaagct cgcggatcgg
      421 tgccgttccg aagatgtcgg tcgatgtagc tcagaataag acgttgcgtc tctcgatcga
      481 gtttttgtat cttcttcgcc gtctcggcat ctatttctac cgaccggacg ctcataaatc
      541 ctcctcggaa aacccgcact ccttcactaa atcctcgaag cgcaccgtct ctcgcttgct
      601 cttcaaccat gccgtatata cttcgagatc ataggcatcc tcgatttttt cgagaagaga
      661 ctcccgggcg agctgggcga tggtcatatt tttctctttc gcgtactcct cgaccgccat
      721 acgctcttcg ggagtgagcc gtaccgtgag tgtcttttcc aaagtcgcca tttcagccac
      781 ctcctttgta ttgcatcgta acacaaaggt atgcgctgtc aactccggac agttgccctc
      841 attaaaaact tatatgaaag aaatcgaaga aattcaaaaa ccggaagaaa tcgttgcatc
      901 ctgcgacgtt gccatcacgc cggacacccc cgcacgaaat ttccgtaccg ccgcggaagc
      961 gaagaacaca aaacgggaga ggctccggcg aagcgtcccc gctccggccg aagcccctcc
     1021 cgctcgcgtc ttcgaaccgc gccccgccct agaagtacag ctcgtactcc tgcggcgtcg
     1081 gggcgttgta gacgtactcg gcttcctcaa gcttccgctt cgtccacagc tccatcagct
     1141 tttcggggaa ggcgggcttc agccattcgt tgtccttttt caggccgtcg agcaccgcgt
     1201 tcaggctcag cgggaagacc ttctcctgct tcgcctccgc gctgctgaag ccgttcgcca
     1261 ccgggtcggc gttccggaga atgccgtcga ctccggcgag caccatcgcc gagagcatgt
     1321 agtgaatgtt cgccgaggcg tcgccggtgc ggtactccac gcggacttcg tccttcttca
     1381 ggtagccggg gatgcgcacc gccgcgctcc gcgacccctt cgcgaaggtc gcgctgatgg
     1441 gggcctcgta gccggggacg agccgccgga agctgttcgt gctggggttc gtgaaggcga
     1501 gcagactccc cgtgaggctg tgctccagca ggccggaggt gtaggcaagc ccctccttcg
     1561 acagaccgtg g
//
LOCUS       DAOTIL010000728         1569 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminobacteriaceae bacterium isolate AMR_MDS_3026
            NODE_94273_length_1569_cov_3.066711, whole genome shotgun sequence.
ACCESSION   DAOTIL010000728 DAOTIL010000000
VERSION     DAOTIL010000728.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868050
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminobacteriaceae bacterium (wastewater metagenome)
  ORGANISM  Aminobacteriaceae bacterium
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae.
REFERENCE   1  (bases 1 to 1569)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1569)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:19:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,224
            CDSs (total)                      :: 3,198
            Genes (coding)                    :: 3,184
            CDSs (with protein)               :: 3,184
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1569
                     /organism="Aminobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_94273_length_1569_cov_3.066711"
                     /isolate="AMR_MDS_3026"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:3034609"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(176..646)
                     /locus_tag="P5201_12620"
     CDS             complement(176..646)
                     /locus_tag="P5201_12620"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014163352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1990204 - oxidoreductase complex
                     [Evidence IEA];
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_function: GO:0048038 - quinone binding [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit B family
                     protein"
                     /protein_id="HRV99432.1"
                     /translation="MNTTLLEKYLDILPKSLWVTTCNSGSCNGCDIEIVATISPRYDI
                     ERFGMKLVGTPRHADVLIVTGPVTKYMKDRLLRIYNQIPDPKAVVVVGNCGASGDVFY
                     KSYNLDGPVDNIIPVDVYVHGCPPRPEAIIEGVAKAVLKLESLKQQKKAGEADS"
     gene            complement(659..997)
                     /locus_tag="P5201_12625"
     CDS             complement(659..997)
                     /locus_tag="P5201_12625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014163353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase"
                     /protein_id="HRV99433.1"
                     /translation="MFGTVYTGFGYWDVSAWIVFFAIAAGLTLWLRAQGREDFKEGTD
                     QDEIYWSGNPVPDDGAEISVPASSAYWGFRKAMEPFYKVLTGMHTGIATDIVGFYVLT
                     VAFMAVFILL"
     gene            complement(997..>1569)
                     /locus_tag="P5201_12630"
     CDS             complement(997..>1569)
                     /locus_tag="P5201_12630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014163354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proton-conducting transporter membrane subunit"
                     /protein_id="HRV99434.1"
                     /translation="LLVLTDAHGMAEYGFTAMKGGIFHIFNYTMYKGLLFLAAGAIYY
                     ATGKRDLNEMGGLARRMPFTTAMFVIAAAAISGLPPFNGFVSKLLIYESVFAVHPALT
                     VVALLTSVLTLASFVKVFQTAFLGPEKSVFTGVREVPSGMLFGMGVLTLAVLGATLFP
                     SWTLSNLVEPAAKALVDQAGYIGAVMGGGL"
BASE COUNT          347 a          480 c          444 g          298 t
ORIGIN      
        1 aagcgcccct tctccaccga cagccagatg tcgcgatact gagcctcgtc tttaagccct
       61 gcggtatggg cgcgcacatc gacgacagcg ccctcgccga agagttcgcg aatacgggca
      121 agaagttctt ccggttcccg ccccgtttcc gcgttgagtc ctttcaccgg aaaagtcatg
      181 agtccgcctc acccgctttc ttctgttgct tcaatgattc cagcttcagc accgccttgg
      241 cgacgccttc gatgatcgcc tcggggcgcg gagggcatcc gtggacatag acgtccacgg
      301 ggatgatatt gtccacaggg ccgtcgaggt tgtacgactt gtagaagacg tctcccgacg
      361 cgccgcagtt ccccaccacc acgaccgcct tcggatcggg tatctggttg tagatgcgca
      421 gcagcctgtc cttcatatat ttcgtcacag ggccggtcac aatgagcaca tcggcgtggc
      481 gcggtgttcc cacaagtttc atgccgaaac gttcgatgtc gtagcgcggg ctgatagtgg
      541 ccacgatttc aatatcgcat ccgttgcacg acccggagtt gcaggtcgtc acccagagcg
      601 atttgggcag aatatccaga tatttctcaa gcagcgtagt gttcatggcg ccctcctcct
      661 acagaagaat gaacacggcc ataaaggcga cggtcagaac gtaaaacccg acaatatccg
      721 tcgctattcc cgtgtgcatt cctgtcagca ccttgtagaa aggctccatg gcttttctga
      781 aaccccaata cgcggaagag gcgggaacgg atatctccgc cccgtcgtcg ggaacggggt
      841 tgccggacca gtaaatctcg tcctgatccg tcccctcctt aaagtcctcg cgcccctggg
      901 cgcggagcca gagcgtcaga ccggcggcga tggcgaagaa gacgatccac gcggagacgt
      961 cccagtagcc gaatccagtg tagacagtgc cgaacatcag agcccacctc ccataacagc
     1021 gcctatatac ccggcctgat caacgagagc cttcgccgcg ggctcgacga gattcgacaa
     1081 ggtccacgac gggaagagcg tagcgccaag aaccgcgagc gtcagcactc ccatgccgaa
     1141 cagcattccc gacggaacct ccctgacgcc tgtgaagacg ctcttttcgg gaccgaggaa
     1201 ggccgtctga aagactttta caaaggaagc cagcgtcagc accgatgtga gcagcgctac
     1261 aactgtcaac gccggatgca cggcgaagac tgactcgtag ataagcagct tcgatacgaa
     1321 accgttgaac ggaggaagac cggagatcgc cgccgccgcg atcacaaaca tggcggtggt
     1381 aaatggcatg cggcgcgcga gaccgcccat ttcattcaag tcgcgcttcc ctgtcgcgta
     1441 gtagatggcg ccggcggcaa ggaagagcag ccccttgtac atggtgtagt tgaagatgtg
     1501 gaagatgccg cccttcatcg cggtgaagcc gtactcggcc atcccgtgcg cgtcggtgag
     1561 gacgagcag
//
LOCUS       DAOTIL010000729         1567 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminobacteriaceae bacterium isolate AMR_MDS_3026
            NODE_94418_length_1567_cov_3.250661, whole genome shotgun sequence.
ACCESSION   DAOTIL010000729 DAOTIL010000000
VERSION     DAOTIL010000729.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868050
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminobacteriaceae bacterium (wastewater metagenome)
  ORGANISM  Aminobacteriaceae bacterium
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae.
REFERENCE   1  (bases 1 to 1567)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1567)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:19:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,224
            CDSs (total)                      :: 3,198
            Genes (coding)                    :: 3,184
            CDSs (with protein)               :: 3,184
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1567
                     /organism="Aminobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_94418_length_1567_cov_3.250661"
                     /isolate="AMR_MDS_3026"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:3034609"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..656)
                     /locus_tag="P5201_12635"
     CDS             complement(<1..656)
                     /locus_tag="P5201_12635"
                     /inference="COORDINATES: protein motif:HMM:NF019349.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FadR/GntR family transcriptional regulator"
                     /protein_id="HRV99435.1"
                     /translation="MGPIKRVRLSERVTDAIETMIAEGAFAPGDRFLSENDLVKKLEV
                     SRSSVREAVRILEVRGRVIVKHGKGIFIAEPPDEDRDPFTGWLKDNKAPIFDHFEVRL
                     IIEPKAARRAAEKADDESVKGIEKAYGAFLASAERGDTAAMIRFDGEFHRLVAKATKN
                     RTLYHLMKTMTKSLPEGWITSLHIPGRVEKTLGEHASILDAIKGGDPERAEEMMTLHL
                     T"
     gene            complement(760..>1567)
                     /locus_tag="P5201_12640"
     CDS             complement(760..>1567)
                     /locus_tag="P5201_12640"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HRV99436.1"
                     /translation="QVLVNLLGNAVKFTEEGEVELSASFQPSAGAEHMGLFTFSVRDT
                     GIGIRKEDREKLFRPFSQADASNTRKYGGTGLGLVISNGILKKMGSALELESEPGNGS
                     RFSFTVSARYEEDVSLPDTPRESKGEVRQEQAFSELSSRAFSLLIVEDLRMNRTLLRL
                     LASRLLPSAAVFEAENGERAVEIFRTHAPDIVFMDMHMPVMNGFEATAAIREFEKSRG
                     THTPIVALTADVLSETRERCFQAGMDDFLPKPIEAESLRAVLERYLSLSF"
BASE COUNT          338 a          451 c          399 g          379 t
ORIGIN      
        1 gtgagatgca aagtcatcat ttcctccgct ctctcgggat cgcccccctt gatcgcatca
       61 agaatggaag catgttctcc aagtgttttt tcaactcttc ccggaatatg gagactggtg
      121 atccaacctt cgggaagaga ttttgtcatc gttttcatca aatgatagag cgtccggttc
      181 ttcgtagctt ttgcgacgag tctgtgaaac tctccgtcga aacgtatcat tgccgcggtg
      241 tctccccgct ccgcgctcgc gaggaatgcc ccgtaggcct tttcgattcc tttgacgctt
      301 tcgtcgtcgg ctttttcagc ggcgcgccgc gcggctttgg gttctatgat caggcgcacc
      361 tcgaaatgat caaaaatcgg ggccttgttg tccttcagcc atcctgtgaa ggggtcgcga
      421 tcctcgtccg gaggttcggc aataaaaata cccttgccgt gcttcacgat cacccttccg
      481 cgaacctcca gaattcggac ggcctcacgg acggaggaac ggctcacctc aagttttttt
      541 acaagatcgt tctcggaaag gaatctgtcc ccgggggcga aagccccctc tgcgatcatc
      601 gtctcaatcg cgtctgttac cctttcggac aggcgtactc tttttatggg gcccatagtt
      661 tcacccgctt ttcgcaagat gagaacatag cggacatctt cttatcagac aagttgattg
      721 tacccctgtt ttccgcaccc tgtcaagaga gtaaaaagtt cagaaagaca aagaaagata
      781 ccgctccagg acggcgcgca gcgattcagc ctctatcggt tttggaagaa agtcgtccat
      841 acccgcctga aaacagcgtt cgcgagtttc ggacagcaca tccgccgtca gggcgactat
      901 cggcgtatgc gtcccgcggg atttctcaaa ctctctgatg gcggcggtcg cctcgaagcc
      961 gttcatcacc ggcatgtgca tatccatgaa gacgatatcc ggcgcgtgcg tacggaatat
     1021 ctcaacagcc cgctccccgt tttccgcctc aaaaacagcg gcggagggga gcaatcgcga
     1081 cgcaagcagc ctcaggagcg tcctgttcat tctgagatct tccactatga ggagcgagaa
     1141 ggctcgtgaa gaaagctcgg aaaacgcctg ctcttgtcgc acttcaccct tcgactcccg
     1201 cggggtgtcc ggcaaagaga cgtcctcctc gtaccgtgcg gaaaccgtga aagaaaagcg
     1261 gcttccattc ccgggctcgc tctcaagttc gagagcgctt cccatttttt ttagaatacc
     1321 attggagata acaagaccga gtcccgttcc gccgtacttt ctcgtgttcg acgcgtccgc
     1381 ctgggaaaac ggacggaaga gcttctctct gtcttctttt cggatgccta ttccggtatc
     1441 acgcacggaa aaggtgaaaa gccccatgtg ttcagcgccg gctgagggct gaaaagaggc
     1501 ggagagttcg acttctccct cctcagtgaa tttgacggcg ttccccagaa gattgacaag
     1561 cacttgc
//
LOCUS       DAOTIL010000730         1565 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminobacteriaceae bacterium isolate AMR_MDS_3026
            NODE_94619_length_1565_cov_2.612583, whole genome shotgun sequence.
ACCESSION   DAOTIL010000730 DAOTIL010000000
VERSION     DAOTIL010000730.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868050
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminobacteriaceae bacterium (wastewater metagenome)
  ORGANISM  Aminobacteriaceae bacterium
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae.
REFERENCE   1  (bases 1 to 1565)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1565)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:19:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,224
            CDSs (total)                      :: 3,198
            Genes (coding)                    :: 3,184
            CDSs (with protein)               :: 3,184
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1565
                     /organism="Aminobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_94619_length_1565_cov_2.612583"
                     /isolate="AMR_MDS_3026"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:3034609"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>639
                     /gene="kduI"
                     /locus_tag="P5201_12645"
     CDS             <1..>639
                     /gene="kduI"
                     /locus_tag="P5201_12645"
                     /EC_number="5.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011109446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008697 -
                     4-deoxy-L-threo-5-hexosulose-uronate ketol-isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0045490 - pectin catabolic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="5-dehydro-4-deoxy-D-glucuronate isomerase"
                     /protein_id="HRV99437.1"
                     /translation="CDFKSYDTERLRKDFLITHLFVPGEIKMTYSHIDRIITGGACPT
                     IPLSLECGKEIGTEFFLQRREMGIINVGAPGSVTVDGERFALKKMDGLYLGAGGKNIV
                     FESDKHDDHAHFYFSSTPAHTSYPNCYIDMEKAKKVKMGEPEKANVRTINQYIHPEVC
                     TTCQLMMGLTQLENGSVWNTMPCHTHERRMEVYFYFDIPQDGVTFHIFGQPE"
     assembly_gap    640..649
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <650..835
                     /locus_tag="P5201_12650"
     CDS             <650..835
                     /locus_tag="P5201_12650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005659758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016861 - intramolecular oxidoreductase
                     activity, interconverting aldoses and ketoses [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-deoxy-glucuronate isomerase"
                     /protein_id="HRV99438.1"
                     /translation="VAAIVRTETRHIVVRNEEAVINPSWSIHSAAGTSNYSFIWSMAG
                     ENQVFTDMDHVPLSAMK"
     gene            999..1226
                     /locus_tag="P5201_12655"
     CDS             999..1226
                     /locus_tag="P5201_12655"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRV99439.1"
                     /translation="MDWGRVPADTVVIESKEITLRDVVQAAADGVDTPEGLMEVLGLE
                     EGQEGTEHLQPILDVFLPAIERLRSGSCGGG"
     gene            complement(1337..>1565)
                     /locus_tag="P5201_12660"
     CDS             complement(1337..>1565)
                     /locus_tag="P5201_12660"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRV99440.1"
                     /translation="SGILSMHEKLHGLGHTVKNAMQRGEGGKAREVLKETVALSERLS
                     ASLLRVVEICRGQGEQEREASGLPALPERTR"
BASE COUNT          345 a          470 c          450 g          290 t
ORIGIN      
        1 cgtgcgactt caagagctac gacactgaac ggctacgtaa ggactttttg ataacgcatc
       61 ttttcgtccc cggagagatc aagatgacgt acagccatat cgaccgtatc atcaccggcg
      121 gcgcctgccc gactatcccc ctgtcgctgg agtgcggcaa ggagatcggg acggagtttt
      181 tcctccagcg gcgcgagatg gggatcatca atgtaggggc tcccggctcg gtcaccgtcg
      241 acggagagcg tttcgccctc aagaagatgg acgggctcta cctcggagcg ggcggcaaga
      301 acattgtctt cgagagcgac aaacacgacg atcatgcgca cttctacttc agcagcaccc
      361 ctgcccatac ttcgtacccc aactgctaca tcgacatgga aaaagcgaaa aaggtcaaaa
      421 tgggcgagcc ggaaaaggcc aacgtccgca ccatcaacca gtacatccac ccggaggtat
      481 gcaccacctg ccagctcatg atggggctca cgcagctcga aaacggcagc gtctggaaca
      541 ccatgccctg ccacacccat gagcggcgca tggaagtgta cttctacttc gacattcccc
      601 aggacggcgt gacgttccat atcttcggcc agcccgaggn nnnnnnnnng tcgccgccat
      661 cgtccgcacc gagacccgcc acatcgtcgt ccgcaacgag gaggccgtca tcaatccgag
      721 ctggtccatc cacagcgcgg cgggcacgtc aaactacagc ttcatctgga gcatggcggg
      781 agaaaaccag gtgttcaccg acatggacca cgttccgttg tcggcgatga aataggaaaa
      841 tgcaaggggc tccattcgaa gaaaataccc tctagcggca tatacgaaaa aacacctatg
      901 gcgcatttgt cgaaagaagg tataatatcg aaagcttttt cttccggagc gccatgttcg
      961 gaaaaagggc tgactttact gaggaaggag gagtcgtcat ggattgggga agagtgcccg
     1021 cggataccgt agtgatcgag agcaaggaga tcacgctgcg cgacgtggtg caggcggcag
     1081 ccgacggcgt cgacacgccc gagggactga tggaagtcct cggccttgaa gaggggcaag
     1141 agggaaccga acacctgcag cccattctgg acgtctttct tccggccatc gaacgcctcc
     1201 ggagcggcag ctgcggcggc gggtgagccg gttgttccgg cagctgttag ctgctgattg
     1261 aagacgaaaa agagggccga ggcatcccct tgcggggggc ttcggccctc ttcttttata
     1321 gaattgtcct tgccgcttac cgcgttcttt ccggaagcgc cgggagcccc gaggcttccc
     1381 gctcctgttc cccctgcccg cggcagatct caaccacgcg gagcagggat gccgaaagac
     1441 gttcgctgag ggctacggtc tccttcagga cctctcgcgc cttgccgccc tccccgcgct
     1501 gcatcgcgtt cttgactgtg tgccccagac cgtgcagctt ctcgtgcatc gagaggatgc
     1561 cgctc
//
LOCUS       DAOTIL010000731         1564 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminobacteriaceae bacterium isolate AMR_MDS_3026
            NODE_94678_length_1564_cov_3.799867, whole genome shotgun sequence.
ACCESSION   DAOTIL010000731 DAOTIL010000000
VERSION     DAOTIL010000731.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868050
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminobacteriaceae bacterium (wastewater metagenome)
  ORGANISM  Aminobacteriaceae bacterium
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae.
REFERENCE   1  (bases 1 to 1564)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1564)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:19:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,224
            CDSs (total)                      :: 3,198
            Genes (coding)                    :: 3,184
            CDSs (with protein)               :: 3,184
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1564
                     /organism="Aminobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_94678_length_1564_cov_3.799867"
                     /isolate="AMR_MDS_3026"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:3034609"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            591..878
                     /locus_tag="P5201_12665"
     CDS             591..878
                     /locus_tag="P5201_12665"
                     /inference="COORDINATES: protein motif:HMM:NF020133.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRV99441.1"
                     /translation="MNEEEAEDERNGILSKLHDKTFCGLLPNDFCTVLLKEHPEYADV
                     FSAFRESGSLGWGITGSGSSAFGLFHQNDGFSRMIRSCRHMQWIRKILFVE"
     gene            885..>1564
                     /locus_tag="P5201_12670"
     CDS             885..>1564
                     /locus_tag="P5201_12670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006300830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosyltransferase family protein"
                     /protein_id="HRV99442.1"
                     /translation="MKGQRTERLIRTSARFLTCPSRQLSLTSLAEDFLVSKTVISDDV
                     AIIDEAFSREGLGGIIVDRGRTGGASFVPRIAPEIKDTLLNDLINLLNSEDRFLPGGL
                     VYYSDILFNPHYALRLGYAMASLFSETRPDIVMTSEVKGIPLAIFTAHAMGIPLAVCR
                     FRNRASDGSAVTVHYPTKNGEVKAMYMGTKQLTRGKRVLIVDDFMRGGSTAAGMLLVA
                     KEFGAEVAG"
BASE COUNT          379 a          373 c          428 g          384 t
ORIGIN      
        1 attcaaggaa ggagctgctc cttttgattc actgtctgca atccgcggcg aaggtgaaca
       61 tcacgcttcg agtcaccaga aaacgtgatg acggttatca tgaacttcgt tctctttttt
      121 atgcgcttcc cgcaattgaa tcattgacaa ttgaacctga ttatggtcat aatgtaaagg
      181 acataatctc cgtaacagga gaaatgattc gcggaagaaa catccttgaa gaagttcttt
      241 ctgcggcgcg aaaaaaaaga ggagtggatc ctctgcgcat acggcttcat aaggtcatac
      301 cccctgggag cggcctcggc gggggaagcg gaaacgccgc agctctgatt tcttggctga
      361 actctcagcg tgaagagaat gaaggaattc gaggcgaaga gattgggtcc gacgttccgt
      421 tttttttgaa cgcagagaaa tgggctttcg tcggaggcag aggtgaacgt gtggaacctc
      481 tctccgggat tatgccgccc tttcacgcag tcgttgtcat tccctcatgg agtatgtcga
      541 cggcaagagc cttttctctt ctcacagccg aatacggcgg ttttttcccc atgaacgagg
      601 aggaagccga agatgagagg aatggtattc tctcaaaatt gcatgataaa acgttttgcg
      661 ggcttctgcc gaatgatttc tgcacagttc ttctgaaaga gcacccggaa tacgccgacg
      721 tttttagcgc attccgggaa agcggatcgc ttggatgggg tatcacagga agcggcagct
      781 cggctttcgg gctgtttcat caaaatgacg gtttttcgcg aatgattcgg agctgcaggc
      841 atatgcaatg gatacgcaaa atcttatttg tggagtgatg atgaatgaaa ggacaacgaa
      901 ccgaacgatt gatccgtact tcggcacgat ttctcacatg cccttcgaga cagctctcgc
      961 tgacatctct ggccgaggat tttcttgtct caaagactgt catcagcgat gacgtcgcga
     1021 tcattgacga ggctttttca agggaaggcc tgggcggcat tatcgtcgac agggggagaa
     1081 ccggaggagc ttcatttgta ccgcgcatag ctcccgaaat caaggatacg ctcctgaacg
     1141 acctcattaa tctgttgaac agcgaggatc gtttcctgcc cggcgggctt gtgtattaca
     1201 gcgatatact ctttaatccg cactacgctc ttcgtctcgg ctatgcgatg gcctcgctct
     1261 tcagtgaaac tcgtccggac attgtcatga catccgaagt aaagggcatt ccgctcgcta
     1321 tttttactgc ccatgcgatg ggtatcccgc ttgcggtctg ccggtttcgt aacagggcaa
     1381 gcgacgggtc ggcggtgaca gttcactacc cgacaaagaa cggcgaggtc aaggctatgt
     1441 acatgggaac gaaacaatta acccgcggga agagagtgct cattgtcgac gactttatgc
     1501 gcggcggaag cactgcggcc gggatgctcc tcgtcgcaaa ggaattcggc gccgaagtag
     1561 cagg
//
LOCUS       DAOTIL010000732         1563 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminobacteriaceae bacterium isolate AMR_MDS_3026
            NODE_94823_length_1563_cov_2.084881, whole genome shotgun sequence.
ACCESSION   DAOTIL010000732 DAOTIL010000000
VERSION     DAOTIL010000732.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868050
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminobacteriaceae bacterium (wastewater metagenome)
  ORGANISM  Aminobacteriaceae bacterium
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae.
REFERENCE   1  (bases 1 to 1563)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1563)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:19:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,224
            CDSs (total)                      :: 3,198
            Genes (coding)                    :: 3,184
            CDSs (with protein)               :: 3,184
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1563
                     /organism="Aminobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_94823_length_1563_cov_2.084881"
                     /isolate="AMR_MDS_3026"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:3034609"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(72..1160)
                     /locus_tag="P5201_12675"
     CDS             complement(72..1160)
                     /locus_tag="P5201_12675"
                     /inference="COORDINATES: protein motif:HMM:TIGR01730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HRV99443.1"
                     /translation="MKKYLVFFLFLAAFGGLILYRRHETLSTMTEVPVTEVIPVRTLS
                     PLPTTFEETVSFAANVEPEETAAVVSKVPGRTVLRVHVNEGDYVKQGQKLAEVDDSLL
                     RQQIAQAEAALGKARTYASTIGADSKRIAALFQENVVSRQQYERMDGEAKVAARQVEE
                     ARAGLNQLRIMLGYHTLEAPVSGIVLSRTVDPGDTVSQLPAFIISRQENVTITGGVPE
                     RSYPSVRVGQKVFVSLDAFPGERFEGVVLRIAPALDPGTRTGKVEVLLPSGGRIKPGM
                     FARVSLKLGERQGYAVPLEAVGRMSGSGESICYVVSGDAALLRKISTGAEKGGQVEIL
                     SGLEPDEIIIATRSEKIRDGVQVKVSDR"
     gene            complement(1157..>1563)
                     /locus_tag="P5201_12680"
     CDS             complement(1157..>1563)
                     /locus_tag="P5201_12680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013047713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HRV99444.1"
                     /translation="TLLASWSFFDGGASSARAREARAAAEEFLFRMEDMKRQIELEIS
                     VARLNCESSLQRISTGRTMVESAEEDYRMALRRYTAQVGTNIDVLDARVALSNARNQL
                     VESVYDSLKARAEMEYAMGVIGSDGRGEERTK"
BASE COUNT          302 a          522 c          427 g          312 t
ORIGIN      
        1 tcccaggagg atcgccgcga gaaagaccat cagcatggcc gtcttgcgct tgacggaaag
       61 ctccgggagg ttcatcgatc gctcaccttc acctgaacgc cgtcgcggat cttctcggaa
      121 cgagtggcga tgattatttc gtcgggttcg agcccgctca gaatctcaac ctgtccgcct
      181 ttttcagcgc ccgtggaaat tttccgcaac agcgccgcgt ccccggagac cacgtaacag
      241 atgctctccc ccgagccgga cattctccct accgcttcaa gcggcacggc gtacccttgg
      301 cgttcgccca gcttgagcga gacgcgggcg aacatgccgg gcttgatgcg gcctcccgag
      361 ggaagcagca cttccacttt tcccgtgcgt gtcccgggat cgagagcggg tgcgatacgc
      421 aggacgactc cttcaaagcg ctccccgggg aatgcgtcga gagagacgaa gaccttctga
      481 ccgacccgta ccgacgggta ggaacgctcg ggtacgcctc cggtaatcgt cacgttctcc
      541 tgccgggaga tgataaaggc gggcaactgg gaaaccgtat ctccggggtc gaccgtccgc
      601 gagaggacga taccggaaac aggcgcctcc agcgtatgat agccgagcat gatccgcagc
      661 tgattcaacc ccgcccgagc ttcctccacc tggcgggccg cgaccttcgc ctcgccatcc
      721 atcctctcgt actgctgcct gctcacgacg ttctcctgaa aaagcgcggc gatccttttg
      781 gagtccgctc cgattgtcga agcgtacgtc cgcgcctttc ccagcgcagc ctcggcctgc
      841 gctatctgct gacgaagaag gctgtcgtcg acctcggcga gtttttgccc ctgcttcacg
      901 taatcccctt cattcacatg cacccgaagc accgtgcgcc cgggaacctt gctcacaacg
      961 gccgcagttt cctccggctc gacgttcgcc gcaaaggaaa cggtttcctc gaaggtcgta
     1021 ggaagaggag aaagagttcg taccggaatg acctccgtca ccgggacttc cgtcatcgtc
     1081 gagagggtct cgtgccgccg gtagagtata agaccgccga acgcagctaa gaagagaaaa
     1141 aataccaggt attttttcat ttagtccgtt cctcccctct tccgtcgctt ccgatgacgc
     1201 ccatagcgta ctccatctcc gccctcgcct tgagcgagtc atagacgctc tccacaagtt
     1261 ggttccgggc attggagagg gcaacgcgcg catcgagcac atcgatattc gttcccacct
     1321 gtgccgtgta gcgccgcagc gccattctgt agtcctcctc cgcgctctcg accatcgtcc
     1381 gccccgtgct gatccgctgg agcgacgatt cacagttcag ccgggcgacg gagatctcaa
     1441 gttcgatctg ccgcttcata tcctccatcc taaagagaaa ctcttccgcg gccgccctgg
     1501 cctcgcgggc tctggcggac gaagcgcctc cgtcgaaaaa gctccaggag gccagcaaag
     1561 tta
//
LOCUS       DAOTIL010000733         1562 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminobacteriaceae bacterium isolate AMR_MDS_3026
            NODE_94894_length_1562_cov_2.604512, whole genome shotgun sequence.
ACCESSION   DAOTIL010000733 DAOTIL010000000
VERSION     DAOTIL010000733.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868050
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminobacteriaceae bacterium (wastewater metagenome)
  ORGANISM  Aminobacteriaceae bacterium
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae.
REFERENCE   1  (bases 1 to 1562)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1562)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:19:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,224
            CDSs (total)                      :: 3,198
            Genes (coding)                    :: 3,184
            CDSs (with protein)               :: 3,184
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1562
                     /organism="Aminobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_94894_length_1562_cov_2.604512"
                     /isolate="AMR_MDS_3026"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:3034609"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..409)
                     /gene="rhuM"
                     /locus_tag="P5201_12685"
     CDS             complement(<1..409)
                     /gene="rhuM"
                     /locus_tag="P5201_12685"
                     /inference="COORDINATES: protein motif:HMM:NF024706.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RhuM family protein"
                     /protein_id="HRV99445.1"
                     /translation="MSEESVVNYWLITAADGELQRVATAEESSVVQIEGGREVRRMVT
                     LYNLDAILAVGYRVRSPRGVQFRRWASTVLAEYLRKGFAMDDERLKNPDGRPDYFDEM
                     LARIRDIRASEKRFYQKVRDLFALAADYDKTDQA"
     gene            815..1027
                     /locus_tag="P5201_12690"
     CDS             815..1027
                     /locus_tag="P5201_12690"
                     /inference="COORDINATES: protein motif:HMM:NF023857.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRV99446.1"
                     /translation="MTLHLDLDGARRSSRKQKRINVSFPEWMLESLDLEASRLGIPRQ
                     AIIKLWLSERLKSASDENGTDQHPVA"
     gene            1223..>1562
                     /locus_tag="P5201_12695"
     CDS             1223..>1562
                     /locus_tag="P5201_12695"
                     /inference="COORDINATES: protein motif:HMM:NF016232.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HRV99447.1"
                     /translation="METNEQRILDLIRKGEGLNLEFKTCRERLNRDVYETVCAFLNRH
                     GGTILLGVQDSGEIAGIAPDALARIRKDFVTAVNNPQKLIPPTYLSIDEVAVEGKTLL
                     RIYVPESSQVH"
BASE COUNT          364 a          410 c          460 g          328 t
ORIGIN      
        1 tggcctgatc ggtcttatcg taatcggcag ccagtgcgaa gagatcgcgc actttttgat
       61 agaagcgttt ctccgaggcg cgaatatcgc ggatgcgcgc cagcatctcg tcgaaatagt
      121 ccgggcggcc gtcggggttc ttcagtcgct cgtcgtccat ggcgaagcct ttgcgcagat
      181 actccgccag cacggtgctg gcccagcgtc ggaactgcac gccgcgcggc gagcgcacgc
      241 ggtaccccac ggcgagaatg gcgtcgaggt tgtaaagcgt gaccatgcgg cgcacctcgc
      301 gcccgccctc gatttgaact accgaggatt cctcggcagt tgccacgcgt tgcagctcgc
      361 cgtcggcggc agttatcaac caatagttta caactgattc ctcgctcaat tcgttctcgg
      421 tgaaaacggc gcttcaggtg cttggcaaga ttctgctcct tgagtacctg aaaattccat
      481 tcagagtata ataatgggcg gacgaacgtg gacaacgtat tggcgtcgcc gctctccgag
      541 gaacaaaaag tgaacgcaac ctcgaaaaac atggtatcga tttccaggat gccagacagc
      601 tttggagcga tcccgatctg ctcgaaattc cggcaaaggt cgaggacgaa cctcgctttc
      661 ttgttatcgg tcgtattgcc ggaaaacact ggtccgccat gatcacgtac cggcaggaac
      721 gagtccggat aatttcggtg agaagatccc gcaaggatgg ggtgcggttc tatgagcaaa
      781 acaatttgac gaagacttcg acaagggcga ggatattacc cttcatcttg atctcgacgg
      841 agcgaggcga tcctcaagga agcagaaacg gataaatgtt tcttttcccg aatggatgct
      901 ggagtcgctg gacttggaag cgagtcgcct gggcatcccc cggcaggcga tcatcaaact
      961 ctggctgtcg gaacggctga aatccgcttc ggacgaaaac ggaaccgatc agcaccccgt
     1021 ggcataggcg aaagaacgac gatcacgcag cagagcgagt ctgtggcgat ttctcgggcg
     1081 gagttcgccg aggggaagga tattccgctg aaggatgtca tgaatgaact ctggtgaatc
     1141 cttggacagt caatctctcc ccggaggtat tttcccagaa cacatatatg atttttcagt
     1201 agacggaaag gcggcagagg gaatggagac gaacgaacag cgaatactcg acctgatccg
     1261 aaagggagag gggctgaatc tcgagttcaa aacctgccgg gagcgcttga accgggatgt
     1321 gtacgaaacc gtctgcgctt ttttgaaccg gcacggcggg acgatcctgc tcggcgttca
     1381 ggattccgga gagatcgccg gtattgcgcc ggatgctctg gcgcgtattc gcaaggactt
     1441 cgtgactgcg gtgaacaacc cgcagaagct cattcccccg acgtatcttt ccatagacga
     1501 ggtcgcggtg gagggcaaaa ctctgttgcg catctatgtt ccggagagtt cacaggtgca
     1561 tc
//
LOCUS       DAOTIL010000734         1561 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminobacteriaceae bacterium isolate AMR_MDS_3026
            NODE_95009_length_1561_cov_1.673971, whole genome shotgun sequence.
ACCESSION   DAOTIL010000734 DAOTIL010000000
VERSION     DAOTIL010000734.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868050
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminobacteriaceae bacterium (wastewater metagenome)
  ORGANISM  Aminobacteriaceae bacterium
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae.
REFERENCE   1  (bases 1 to 1561)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1561)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:19:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,224
            CDSs (total)                      :: 3,198
            Genes (coding)                    :: 3,184
            CDSs (with protein)               :: 3,184
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1561
                     /organism="Aminobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_95009_length_1561_cov_1.673971"
                     /isolate="AMR_MDS_3026"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:3034609"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..703
                     /locus_tag="P5201_12700"
     CDS             <1..703
                     /locus_tag="P5201_12700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008520275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="2-hydroxyacyl-CoA dehydratase family protein"
                     /protein_id="HRV99448.1"
                     /translation="EYIGKVAGRAITDDALRESIALRNRERRALQSLWELSALDEPPL
                     SGLEVHQFGEYMQYRFDKRKDVPWLEEQVRSIRAAWEGGERREHGKKPRIMITGCPTG
                     GVVKVIEAVEKAGAVVVCYENCSGQKELGYLVDETKDPMEALAEKYLSIGCSIMSPNE
                     NRMNSLRALAERYKIDGVVEVLLTACHTYAVESYSVRNLVQSLGKKYLLVETDYSEGD
                     KEQIATRMDAFVEMM"
     gene            740..>1561
                     /locus_tag="P5201_12705"
     CDS             740..>1561
                     /locus_tag="P5201_12705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006301775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HRV99449.1"
                     /translation="MLKKLGIMALAVFLMAGATFASDLEPSGKGMRVWFDAGGSAGEP
                     YSTILINGAKQAAADLGVELTIVYSDWNAEKMLENFKNGLAARPTGYVVIGVPGDDGF
                     APLVDEAFADGMIVTAVDTELPRLQAKYQSRGFGYIGPDGWKQGEDMAKEILRRKEFK
                     PGDRAFVWGLKRLESRGRRARAILEVFEKAGLKVDYLEISDEVNKDAALGAPVLAGYL
                     AANPDCKLIVLDHGALTGQTANFLRGAGLQPGDVYATGFSLNPATAASIESGYLNL"
BASE COUNT          330 a          429 c          540 g          262 t
ORIGIN      
        1 ggagtatatc gggaaggttg ccggacgcgc gatcacggac gacgccctgc gcgagtccat
       61 cgccctcaga aacagagagc gaagagcgct gcagtccctc tgggagctga gcgcgctgga
      121 cgaacctcct ctttccggtc ttgaggtgca tcagttcggc gagtacatgc agtatcgctt
      181 cgacaagcgg aaggacgttc cctggctcga ggaacaggta cggagcatcc gcgccgcgtg
      241 ggagggcggc gagcgtaggg agcacggaaa gaagccgagg atcatgatca caggatgccc
      301 gacgggcggc gtcgtgaagg tgatcgaggc cgtagagaag gccggggccg tcgtggtctg
      361 ttacgagaat tgcagcggac agaaggagct cggctacctt gtggacgaga ccaaagaccc
      421 catggaggcg cttgcggaga agtacctctc aataggctgc tccatcatga gccccaacga
      481 gaatcgcatg aattcgctgc gtgcgctcgc ggagcgctac aagatcgacg gcgtggtcga
      541 ggtgctgctc accgcctgcc atacgtacgc ggtggagtcc tattccgtgc ggaatcttgt
      601 gcagtccctc gggaaaaagt acctgctcgt ggagacggac tattcggagg gcgacaagga
      661 gcagatcgcc acgcggatgg acgccttcgt cgagatgatg taacccccgc gttcgcggga
      721 aaacgaaagg agaagagaga tgttgaaaaa actgggaatc atggcgcttg cggtctttct
      781 tatggcaggg gcgacgttcg cgtccgacct cgaaccgagc ggcaagggga tgcgggtctg
      841 gttcgacgcg ggcggttcgg cgggcgagcc gtactccaca attttgatca acggcgcgaa
      901 gcaggcggcc gccgacctcg gagtcgaact gacgatcgtc tactccgact ggaacgcgga
      961 gaagatgctg gagaacttca agaacggatt ggccgcccgc ccgactgggt atgtcgtgat
     1021 aggcgttccg ggagacgacg gattcgcccc cttggtcgac gaggcgttcg ccgacggcat
     1081 gatcgtaacg gctgtggaca ccgaacttcc gcgccttcag gcgaagtatc agtccagagg
     1141 tttcggctac atcggccccg acggctggaa gcagggcgag gatatggcga aggagatcct
     1201 tcggcgcaag gagttcaagc ccggagacag ggcgttcgtc tggggtctga agcgtctcga
     1261 aagcagaggt cgcagggcgc gggcgatact ggaggtcttc gaaaaggccg ggctgaaggt
     1321 ggactatctg gagatttccg acgaggtcaa taaggacgcc gcgctcggcg ctccggtgct
     1381 cgccggatac cttgcggcca acccagactg taagctgatc gtgctcgatc acggcgcgct
     1441 gacgggacag accgcgaact tcctcagagg agcggggctt caaccgggcg acgtctacgc
     1501 gaccggcttc agcctgaacc cggcgacggc ggcctccatc gagagcggct acctgaacct
     1561 g
//
LOCUS       DAOTIL010000735         1559 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminobacteriaceae bacterium isolate AMR_MDS_3026
            NODE_95158_length_1559_cov_2.142952, whole genome shotgun sequence.
ACCESSION   DAOTIL010000735 DAOTIL010000000
VERSION     DAOTIL010000735.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868050
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminobacteriaceae bacterium (wastewater metagenome)
  ORGANISM  Aminobacteriaceae bacterium
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae.
REFERENCE   1  (bases 1 to 1559)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1559)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:19:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,224
            CDSs (total)                      :: 3,198
            Genes (coding)                    :: 3,184
            CDSs (with protein)               :: 3,184
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1559
                     /organism="Aminobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_95158_length_1559_cov_2.142952"
                     /isolate="AMR_MDS_3026"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:3034609"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1136
                     /gene="flhB"
                     /locus_tag="P5201_12710"
     CDS             <1..1136
                     /gene="flhB"
                     /locus_tag="P5201_12710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009201671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="flagellar biosynthesis protein FlhB"
                     /protein_id="HRV99450.1"
                     /translation="QFFSQERTEPATPRKRRKEREEGRVAKSQDLGAAVVILTGLFAL
                     LVFGRFMYSYMRDFLVEMIAFMGGSTLREAGWFAVLSRESIPASILPWIPLGLMVAIG
                     GLIVTVAQVGIELTTKPLIPKMDRFNPVSGLKKVISLRSLVELVKGLLKASLFAAVIY
                     FALQKDTPELVAAIRYPLEIGVSQLFSKLLSLSFRLAFLLLVIAIFDYGYQKWEFERS
                     IKMSKQELKEEYKQMEGDPQIKSKIRQKQRELARSRMMSSVPKADVVITNPTHLAVAL
                     EYNREIMEAPVLVAKGSDFLARKIRELAEEHGVPVVENKPLARAIYESMEIGEEIPEH
                     LYKAMAEVLAFIYKLKGAKNRGASPPRTSVSSTEGPPQAPIFM"
     gene            1170..>1559
                     /locus_tag="P5201_12715"
     CDS             1170..>1559
                     /locus_tag="P5201_12715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009201670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FHIPEP family type III secretion protein"
                     /protein_id="HRV99451.1"
                     /translation="MGKVQESTAGMSPFKKMLHYSDIGMAFLVILIVVMMVIPLPTWL
                     LDVMLSANITIGVVVLLSTFYAQRALDLAAFPTMLLIVTLFRLALNVSTTRLILLQAD
                     AGDVVRAFGTFVVGGNYVVGAVVFLILV"
BASE COUNT          315 a          402 c          466 g          376 t
ORIGIN      
        1 ttcagttctt ctctcaggaa cgaactgaac ccgctacgcc gcgaaaacgc cggaaggagc
       61 gggaggaggg gcgggtcgcc aagagccagg atcttggggc ggccgttgtg atcctgacgg
      121 ggcttttcgc gcttctcgtc ttcggccgat tcatgtattc gtatatgcgg gactttcttg
      181 tcgaaatgat cgcgttcatg ggtgggagca cgctgcggga ggccgggtgg ttcgccgttc
      241 tgtcccgcga atcgattccg gcgtcgattc tgccctggat tcctcttggg cttatggtgg
      301 cgataggggg tcttattgtt accgtggcgc aggtgggaat agagcttacg acgaagccgc
      361 tgattccgaa aatggaccgc ttcaatccag tctccgggtt aaaaaaagta atctccctcc
      421 gttcccttgt ggagcttgtt aaagggctgc tgaaggcgtc gctctttgct gcggtgattt
      481 atttcgcgtt gcagaaagat actcccgagc ttgtcgccgc gattcgttat ccgctggaaa
      541 tcggcgtttc gcagctcttt tcgaagcttc tcagcctcag cttccgcctt gcctttcttt
      601 tgctcgtcat tgcgatattt gactacggct atcagaagtg ggagtttgaa cgctcgatca
      661 agatgagcaa gcaggagctg aaagaggagt ataagcagat ggaaggagat ccccagatca
      721 agagcaagat ccgccaaaag cagcgcgagc tggcacgcag caggatgatg tcctccgttc
      781 ccaaggcgga cgttgtcatt accaacccga cgcacctggc cgtggcgctt gaatacaacc
      841 gcgagattat ggaggcgcct gttctggttg ccaagggaag cgattttctt gcgaggaaga
      901 tacgtgagct cgccgaggaa cacggcgtcc ctgtggtgga gaacaagccg cttgccaggg
      961 ctatctacga atcaatggag atcggagagg aaatccccga acacctgtac aaggctatgg
     1021 ccgaggtgct ggccttcatt tacaagttga agggagcaaa aaatagaggc gcttctccgc
     1081 cgcggacttc tgtttcttcg accgagggtc cgccccaagc gccgattttc atgtaaaata
     1141 ggaaacagcc aaggaagcga tacggaagga tgggaaaggt gcaggaatcg actgcgggga
     1201 tgtctccgtt caaaaaaatg cttcactact cggatatagg tatggccttc ctcgtgatcc
     1261 tcatcgttgt gatgatggtc attccgctgc cgacgtggct tctggacgtg atgctttcgg
     1321 ccaatatcac tatcggcgtg gtggtgctgc tgtcgacgtt ctacgcgcag cgtgcgctcg
     1381 acctcgcggc ctttcctacg atgctgctga tcgttacgct cttccggctt gcactgaacg
     1441 tctctacgac gcgtctcatc ctcctgcagg cggatgccgg cgatgtcgtc agggcgttcg
     1501 gcacgtttgt cgtcggaggg aactacgtcg tgggcgcggt agtcttcctg atactcgtc
//
LOCUS       DAOTIL010000736         1558 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminobacteriaceae bacterium isolate AMR_MDS_3026
            NODE_95198_length_1558_cov_3.596806, whole genome shotgun sequence.
ACCESSION   DAOTIL010000736 DAOTIL010000000
VERSION     DAOTIL010000736.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868050
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminobacteriaceae bacterium (wastewater metagenome)
  ORGANISM  Aminobacteriaceae bacterium
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae.
REFERENCE   1  (bases 1 to 1558)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1558)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:19:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,224
            CDSs (total)                      :: 3,198
            Genes (coding)                    :: 3,184
            CDSs (with protein)               :: 3,184
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1558
                     /organism="Aminobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_95198_length_1558_cov_3.596806"
                     /isolate="AMR_MDS_3026"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:3034609"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            115..510
                     /locus_tag="P5201_12720"
     CDS             115..510
                     /locus_tag="P5201_12720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009200818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase domain-containing
                     protein"
                     /protein_id="HRV99452.1"
                     /translation="MTTAIERTLTDFFEEEQSVRLAFLMGSFAKGTARPDSDVDVAAL
                     FDRTPDFMETLDLRERLSALLRRETDLVVLNDAGPIIGMQALKTGIRLFARKNEYEGF
                     FVRTLNEYDDLKRLRAPIEEAVLKRRIYA"
     gene            503..700
                     /locus_tag="P5201_12725"
     CDS             503..700
                     /locus_tag="P5201_12725"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRV99453.1"
                     /translation="MPDKDVVLAKVATIQRCLARIRDVTSLDPARLYDGYRAMAMDAE
                     REKEAAEWCDALSRGATDEAR"
     gene            complement(718..>1558)
                     /locus_tag="P5201_12730"
     CDS             complement(718..>1558)
                     /locus_tag="P5201_12730"
                     /inference="COORDINATES: protein motif:HMM:NF025019.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF4143 domain-containing protein"
                     /protein_id="HRV99454.1"
                     /translation="DFSHLMAALSPLQRHFNRYLTVGGFPELALSQDDAFSQRMLRED
                     VVDKVIKRDILALFNVRNPLQLEKVFLYLCMHSSGIVNMTTMSKELDGMNKSTLTNYI
                     QFLKEANLIYISDPVGVDGKGVLKGRPKIYVADAAIRSAVLMLENAIADAGETGIMVE
                     TTVYKHIAAFYSAANARVGYYRRVKDGEKEVDVVVELPAGRILCEVKYRENTHVKGTD
                     AIIELADDERANVLGALIITKRAEDYGVAAHATRTPVVRIPAPAFLYLLGNAEKEGDA
                     AKM"
BASE COUNT          319 a          458 c          453 g          328 t
ORIGIN      
        1 acggacgccg ccgctacgcc catcttttgg ggaacggagc gtagagtcag actgccggga
       61 acgctttgtg atatccttct atggaaatga agttattgaa agaggcgatc cgatatgacg
      121 acggccatcg agcgaacgct cacggacttc ttcgaggagg agcaatccgt ccgcctcgct
      181 ttcctgatgg gttccttcgc caaggggacg gcccgtccgg acagcgatgt ggacgtcgcc
      241 gcgcttttcg accggactcc ggacttcatg gagacactcg atcttcggga gcggctttcg
      301 gcgttgctcc gccgcgagac cgatctggtg gtgctgaacg acgcggggcc gatcatcggg
      361 atgcaggcgc tgaagaccgg gatcaggctc ttcgcccgga aaaacgagta cgaggggttc
      421 ttcgtccgca cccttaacga gtacgacgac ctgaagcgcc tccgcgctcc gatcgaagaa
      481 gccgtgctga agaggagaat ctatgcctga caaagacgtg gtgttggcaa aagtcgcgac
      541 catccaaagg tgtctggcgc ggatacgcga cgtcacgtcg ctcgatccgg cacgtctcta
      601 cgacgggtac agggccatgg caatggatgc ggagcgcgaa aaagaggctg cggaatggtg
      661 cgacgcgctt tcgaggggcg cgaccgatga agcacggtaa gcgctgaaag cgagactcta
      721 catcttcgcg gcatcccctt ctttttcggc gtttccgagg aggtagagaa atgcgggggc
      781 gggtatgcgc acgacgggcg tccgcgttgc atgggcggcg actccgtaat cctcggcccg
      841 tttcgtgatg atcagcgctc ccagtacatt cgcgcgctcg tcgtccgcga gttcgatgat
      901 cgcgtccgtc cctttgacat gcgtattctc gcggtatttc acttcgcaca gtatccgacc
      961 cgccggaagc tccaccacca cgtccacctc tttttctccg tccttgaccc tcctgtagta
     1021 gccgacgcgc gcgttcgccg cagaatagaa cgcggcgatg tgcttataga ccgttgtttc
     1081 caccatgatc cccgtctccc cggcgtcggc gatcgcgttc tccagcatca gcacagcgct
     1141 tcgtatagcg gcatcggcca catagatctt cggtcgcccc ttcagtacac ccttcccgtc
     1201 gacacctacg ggatcgctga tatagatcag gttcgcctct ttcaggaact gaatatagtt
     1261 cgtcaacgtg ctcttgttca ttccgtccag ctccttggac atcgtcgtca tgttcacgat
     1321 gccggaagag tgcatgcaga ggtacaagaa gactttttcg agctggagag ggttccgtac
     1381 attgaaaaga gccagaatat cccgtttgat caccttgtcg acaacatcct cgcgcagcat
     1441 cctctgagaa aaggcgtcat cctgcgacag cgcaagttcc ggaaagccgc cgaccgtcag
     1501 gtacctatta aagtgacgct gaagcggcga aagggcggcc atcagatggc tgaaatcg
//
LOCUS       DAOTIL010000737         1556 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminobacteriaceae bacterium isolate AMR_MDS_3026
            NODE_95383_length_1556_cov_3.592272, whole genome shotgun sequence.
ACCESSION   DAOTIL010000737 DAOTIL010000000
VERSION     DAOTIL010000737.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868050
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminobacteriaceae bacterium (wastewater metagenome)
  ORGANISM  Aminobacteriaceae bacterium
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae.
REFERENCE   1  (bases 1 to 1556)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1556)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:19:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,224
            CDSs (total)                      :: 3,198
            Genes (coding)                    :: 3,184
            CDSs (with protein)               :: 3,184
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1556
                     /organism="Aminobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_95383_length_1556_cov_3.592272"
                     /isolate="AMR_MDS_3026"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:3034609"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..395
                     /locus_tag="P5201_12735"
     CDS             <1..395
                     /locus_tag="P5201_12735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013048780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="sodium-dependent transporter"
                     /protein_id="HRV99455.1"
                     /translation="IAALTSAISLLEVVSAYFIDKGWSRPQAAIIMGLLIFALGIPSA
                     MSLAGAPKVAGKDFLDAMDFISSNVLLPLGGVFISLFVGWFWTSDAEKEVTNEGTLTF
                     GLMSMWIWVCRVIAPAAILYIFYTGLKW"
     regulatory      560..662
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00504"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="glycine riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="glycine"
                     /db_xref="RFAM:RF00504"
     gene            767..1180
                     /locus_tag="P5201_12740"
     CDS             767..1180
                     /locus_tag="P5201_12740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013048779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GrdX family protein"
                     /protein_id="HRV99456.1"
                     /translation="MFCRRMLVTNNPLLNEQISSRDFVEGNSLDVLIRTRNLIHKGWV
                     LLTHPLYGNLRPHQHPYRSILMERASDSSSSVDPLSVEYIENAIAIYSAQSSRILSVE
                     GMPEDVRQDFAFIDADLMKESLSRYRMFIREVSVN"
     gene            1205..1525
                     /locus_tag="P5201_12745"
     CDS             1205..1525
                     /locus_tag="P5201_12745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008712805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin domain-containing protein"
                     /protein_id="HRV99457.1"
                     /translation="MVELTKENFDVEVKESALPVLVDFWGPKCGPCMALLPNVHTMAD
                     EYEGKVKFCSVDVSENRRVAIANKVMGLPTFLFWKDGAEVTRISGGDVTLEKIKENVE
                     ALLK"
BASE COUNT          337 a          360 c          428 g          431 t
ORIGIN      
        1 tcatagcggc gctgacttct gcgatttctc tgctggaagt tgtttccgca tactttatcg
       61 acaaggggtg gagcagaccc caggctgcga tcatcatggg cctgctgatc ttcgcccttg
      121 ggattccttc cgccatgtct ctggcagggg ccccgaaggt tgccggaaag gacttcctgg
      181 acgcgatgga ctttatctcc tcgaacgtcc tgcttccgtt gggaggagtt ttcatctcgc
      241 tcttcgtagg ctggttctgg acctccgatg cggagaaaga ggtaaccaac gaaggcacgc
      301 tcacctttgg tctgatgagc atgtggatct gggtttgccg tgttattgct cccgcagcga
      361 ttctgtacat cttctacacc ggtttgaagt ggtaacggtc ctctgaactg cagtacgaag
      421 tgtatttcgc ttcgtacttt gtcgaggtga gaatgccgga atccgaagag attccggcat
      481 tttcgttttt tttgctccgt ctttcacaaa aagggcttga cgcctttttt tcattgtgta
      541 aaatgaagag tgtttccgga tgaacaccgc aagagagact ttcctgcgtt tgcggagagc
      601 gccgaagggg caataccctc tatagggtgg aaactctcag gcgaaaggat tgcggtcgga
      661 cgggtctctg aatagagtag ctcccctgtt tgtttttttc tctttccttt ggggctatgc
      721 gacagagaat gtcttatctg tgctctactt ttgaggaggc agccggatgt tctgtcgcag
      781 aatgcttgtc acaaataacc cgcttctgaa tgaacaaatt tcttcccgcg actttgtcga
      841 agggaattcc cttgatgtcc ttattcgaac aagaaatctc atccacaaag gatgggttct
      901 tcttacgcat cctctttacg gaaatcttcg tccgcatcaa catccatacc gttctatcct
      961 gatggaacgg gcatcggatt catcgtcttc cgtcgatcct ctctccgttg agtatatcga
     1021 aaacgccatt gcaatttatt ccgcacaatc gtcacgaatc ctctcggtcg agggaatgcc
     1081 tgaggatgtg cgtcaggatt ttgcattcat cgatgctgat ttgatgaagg aaagtctttc
     1141 ccggtatcgt atgttcattc gggaagtttc ggtgaattga gtctctaaaa ggaggtgaag
     1201 agaaatggtt gaattgacga aggagaattt cgacgtcgaa gtcaaggaga gcgcgcttcc
     1261 tgtgcttgtc gacttttggg ggccgaaatg cggaccctgc atggcgctgc ttcctaacgt
     1321 ccataccatg gcggacgagt atgagggcaa ggtgaaattt tgctcggtgg acgtatcgga
     1381 aaatcgccgg gtggcgatcg cgaacaaggt tatggggctt cctaccttcc tgttctggaa
     1441 ggatggagcg gaggtgaccc gtatcagcgg cggagatgtc acgttggaga agatcaagga
     1501 gaacgtcgag gctctcttga agtagccttc gacgcctgta aaagggggga gacaga
//
LOCUS       DAOTIL010000738         1554 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminobacteriaceae bacterium isolate AMR_MDS_3026
            NODE_95576_length_1554_cov_2.783856, whole genome shotgun sequence.
ACCESSION   DAOTIL010000738 DAOTIL010000000
VERSION     DAOTIL010000738.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868050
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminobacteriaceae bacterium (wastewater metagenome)
  ORGANISM  Aminobacteriaceae bacterium
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae.
REFERENCE   1  (bases 1 to 1554)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1554)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:19:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,224
            CDSs (total)                      :: 3,198
            Genes (coding)                    :: 3,184
            CDSs (with protein)               :: 3,184
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1554
                     /organism="Aminobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_95576_length_1554_cov_2.783856"
                     /isolate="AMR_MDS_3026"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:3034609"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..467
                     /locus_tag="P5201_12750"
     CDS             <1..467
                     /locus_tag="P5201_12750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006301718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="(2Fe-2S)-binding protein"
                     /protein_id="HRV99458.1"
                     /translation="RQTTLSVEPRATLLRALREYGVTSVKRGCEEGECGTCTVVVDDL
                     AQKSCMMLALEAEGKSVLTVEGLVGPKGELHPIQQAFIEEGAIQCGFCTPGMIMTTYA
                     FLRKTPDPSEEEIRAALAGNLCRCTGYIPIFRAVQKAAKTMRELGHLPGGAR"
     gene            484..>1554
                     /locus_tag="P5201_12755"
     CDS             484..>1554
                     /locus_tag="P5201_12755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006584003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HRV99459.1"
                     /translation="MKQTVTRNGVGAWTTRKYDLEKAAGTLAFTDDLRFGPELLHARA
                     FRSTKAHAEILSVDVSEAMKVKGVVKVITGKDFIHRFGLYLMDRTPLAIGKVRYVGEP
                     VALVIGETEEAAEEGMLKVAAKYKDLPPIFDPVKAATDNSVLVHPELETYDHVPFITP
                     FPGTNIANWFRIRRGDVDKAFAEAEHVVEEAVNCPQIAHGFLEPHCCICKEDPATGNL
                     TIWSSAQSAFALREIVAKGLDYPLNKIHVIAPPIGGGFGGKAGMTVEALCLAGAMNPE
                     IKGRPVKLYIPREEVMLTSWVRQAYVSKIKLAIDKEGKITAIKNTFYFDTGISAEYGA
                     NPVRSAGYSSTGCYYVPNVWTDS"
BASE COUNT          292 a          509 c          495 g          258 t
ORIGIN      
        1 gcagacagac gaccctttcg gtcgagcccc gcgccacgct gctgcgggcg cttcgggagt
       61 atggggttac cagcgtgaag agaggctgcg aagagggaga gtgcggcacg tgcaccgtgg
      121 tggtcgacga cctcgcccag aagtcgtgca tgatgctcgc cctggaggcg gagggaaagt
      181 ccgtccttac cgtcgagggg cttgtcggcc ccaaggggga gcttcacccg attcagcagg
      241 cgttcatcga ggagggggcg attcagtgcg gtttctgcac ccccgggatg atcatgacga
      301 cctacgcctt cctccggaag acccccgatc cctccgagga ggagatccgc gcggcgctgg
      361 cgggcaatct ctgccgctgc accggctaca ttcctatttt ccgcgccgtt cagaaggccg
      421 cgaagacgat gcgcgagctg gggcatttgc ccggcggcgc gcgctgacag ggagggaacc
      481 gcgatgaaac agactgtgac gcgcaacggc gtcggcgcgt ggaccacccg gaagtacgac
      541 ttggagaagg ccgccggaac tctcgccttc accgacgatc tccgtttcgg ccccgagctg
      601 ctccacgcca gggctttccg ctcgaccaag gcgcatgcgg agatcctctc cgtcgacgtc
      661 tccgaggcca tgaaggtcaa gggtgtggta aaggtgatca ccggcaagga cttcatccac
      721 cgcttcggcc tttaccttat ggaccgtacg ccgctggcca tcggaaaggt ccgctacgtc
      781 ggcgagcccg tggcgctcgt gatcggcgag accgaggagg ccgccgagga ggggatgctc
      841 aaggtcgccg cgaagtacaa ggacctgccc cctatcttcg atcccgtcaa ggccgctacg
      901 gacaactccg tcctcgtgca ccccgagctg gagacatacg accacgtgcc gttcatcacg
      961 ccgttccccg ggacgaacat cgccaactgg ttccgtattc gacgcggcga cgtggacaag
     1021 gccttcgccg aggcggagca tgtcgtcgaa gaagccgtca actgtccgca gatcgcccac
     1081 ggttttcttg agccgcactg ctgcatctgc aaggaggacc cggccacggg caacctgacg
     1141 atctggtctt ccgcccagtc ggccttcgcg ctccgcgaga ttgtggcgaa ggggctcgac
     1201 tatccgctca acaagattca cgtgatcgcc cctcccatcg gcgggggttt cggcggcaag
     1261 gccggaatga ccgtcgaggc gctctgcctc gcgggcgcga tgaatccgga aatcaaggga
     1321 cgcccggtga agctctacat cccccgcgaa gaggtgatgc tcacctcctg ggtgcgccag
     1381 gcctacgtct ccaagatcaa gctcgccatc gacaaagagg gcaagatcac cgcgatcaag
     1441 aacaccttct acttcgacac gggcatctcc gccgagtacg gcgcgaaccc cgtgcgcagc
     1501 gccgggtact cctcgacggg gtgctactac gtgcccaacg tctggacgga cagc
//
LOCUS       DAOTIL010000739         1553 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminobacteriaceae bacterium isolate AMR_MDS_3026
            NODE_95656_length_1553_cov_2.660881, whole genome shotgun sequence.
ACCESSION   DAOTIL010000739 DAOTIL010000000
VERSION     DAOTIL010000739.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868050
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminobacteriaceae bacterium (wastewater metagenome)
  ORGANISM  Aminobacteriaceae bacterium
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae.
REFERENCE   1  (bases 1 to 1553)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1553)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:19:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,224
            CDSs (total)                      :: 3,198
            Genes (coding)                    :: 3,184
            CDSs (with protein)               :: 3,184
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1553
                     /organism="Aminobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_95656_length_1553_cov_2.660881"
                     /isolate="AMR_MDS_3026"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:3034609"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..253)
                     /locus_tag="P5201_12760"
     CDS             complement(<1..253)
                     /locus_tag="P5201_12760"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OPT/YSL family transporter"
                     /protein_id="HRV99460.1"
                     /translation="MNEQNRYARSFGEQCTPRALVTGALGAVVITASSMYVALRMGAL
                     PWPTIFVAILSLTVLKALGRTNLNEINVTHTAMSAGGMVA"
     gene            complement(291..1475)
                     /locus_tag="P5201_12765"
     CDS             complement(291..1475)
                     /locus_tag="P5201_12765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013049235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004180 - carboxypeptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HRV99461.1"
                     /translation="MEHILEEAKRISAEITEWRRHLHQTPELGLETPKTAAYIVQELR
                     KMGAEDICEHIGGWGVAALVKGEKPGKTLAIRADCDALPIKEETGLPFASKNGLMHAC
                     GHDAHTAMALGAAKLLLAHKAELAGTVKFIFQPAEENVQGAKAMIDDGVMENPHVDAL
                     IGLHTGCLWKEIGPGEIGVRFGSLMASVDRFVVTFKGKGGHGATPHLTVDPVTMTATA
                     VLELQTILSRELSPLDSAVLTIGRIEGGRAFNIIAENCVIEGTVRTLNPATRAFVEDR
                     LRVVVSRVAEGMRGVAEVEYESGPPALINTADFTRKFHAFAAELAGAEKVKEISEPTM
                     GGEDVAYFLEMVPGTFFVHGACSPEKGQVYPHHNPKFDVDEETFPLGTALFAGFALCW
                     QQ"
BASE COUNT          252 a          520 c          450 g          331 t
ORIGIN      
        1 cagccaccat tccgcccgcg gacatggcgg tatgcgtcac gttgatttcg ttcagattcg
       61 ttctcccgag cgccttcagg acggtcagtg agaggatggc gacgaagatc gtcggccacg
      121 ggagcgcccc catccgcaac gccacgtaca tcgagctcgc cgtgatgaca actgctccaa
      181 gggcgcccgt gacaagtgcg cgcggcgtgc actgttcgcc gaaactgcgc gcgtaccggt
      241 tctgttcgtt catacgattc cctcccaaca agtgaagtgc ttttttctgc ctactgctgc
      301 cagcaaagcg cgaatcctgc gaagagggcg gttcccagcg ggaaggtctc ctcgtcgacg
      361 tcgaatttcg ggttatggtg cgggtacacc tgtcccttct ccgggctgca tgcgccgtga
      421 acgaagaagg ttccggggac catttcgagg aagtaggcca catcttcgcc gcccatcgtc
      481 ggctcgctga tctccttcac cttttccgct ccggcaagct ccgccgcgaa ggcgtggaat
      541 ttgcgtgtga agtcggcagt gttgatgagg gcgggagggc ccgactcgta ctccacttcc
      601 gccaccccgc gcatcccctc ggcgacgcgc gaaacgacta cgcgaagccg atcttctacg
      661 aaggcgcgag tggcgggatt cagcgtccgg acggtaccct cgatgacgca gttctccgcg
      721 atgatgttga acgccctgcc tccctcgatt cgtccgatgg tgagcacggc cgagtcgagc
      781 ggagagagtt cgcggctcag gatcgtctga agctcgagca ccgccgtggc ggtcatcgtc
      841 acggggtcga cggtgaggtg cggcgtcgcg ccgtgccccc ccttcccttt gaaggtgacg
      901 acgaagcggt cgaccgacgc catcagcgag ccgaatcgga cgccgatctc ccccgggccg
      961 atctctttcc acagacagcc ggtatgcagg ccgataaggg cgtccacgtg cggattctcc
     1021 atcacgccgt cgtcgatcat cgccttggcc ccctggacgt tctcctcggc gggctggaag
     1081 atgaacttca ccgtacccgc cagctcggcc ttgtgcgcca gcagcagctt cgccgcgcca
     1141 agcgccattg cggtgtgcgc gtcgtgcccg caggcgtgca tgaggccgtt cttcgaggca
     1201 aacggtagtc ccgtttcctc cttgataggc aaggcgtcgc agtcggcgcg gatcgccagc
     1261 gtcttccccg gtttctcccc tttcacgagc gccgcgacgc cccatccgcc gatatgctcg
     1321 catatatcct ccgcccccat ctttctcagt tcctggacga tataggctgc cgtcttcggc
     1381 gtctccagcc caagttccgg cgtctggtgc agatgccgcc gccactccgt gatctccgcg
     1441 gagatcctct ttgcttcttc aagaatgtgc tccattcctc cccctccgtt cgtgcaggat
     1501 gattttcgct ccattctaca tcattgagat tcttttctct tctgtcggaa gag
//
LOCUS       DAOTIL010000740         1552 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminobacteriaceae bacterium isolate AMR_MDS_3026
            NODE_95739_length_1552_cov_2.682031, whole genome shotgun sequence.
ACCESSION   DAOTIL010000740 DAOTIL010000000
VERSION     DAOTIL010000740.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868050
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminobacteriaceae bacterium (wastewater metagenome)
  ORGANISM  Aminobacteriaceae bacterium
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae.
REFERENCE   1  (bases 1 to 1552)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1552)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:19:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,224
            CDSs (total)                      :: 3,198
            Genes (coding)                    :: 3,184
            CDSs (with protein)               :: 3,184
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1552
                     /organism="Aminobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_95739_length_1552_cov_2.682031"
                     /isolate="AMR_MDS_3026"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:3034609"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..895)
                     /locus_tag="P5201_12770"
     CDS             complement(<1..895)
                     /locus_tag="P5201_12770"
                     /inference="COORDINATES: protein motif:HMM:NF012372.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA cytosine methyltransferase"
                     /protein_id="HRV99462.1"
                     /translation="MGTEKFAVVDLFSGVGGMSFGFHKHPLFSLAAAADAELGKPSSG
                     AGALQCNSAYRLNTGVCPTKLDLSDTPPELLRTALGLPETMRVSVLSACPPCTGFSRA
                     NPENHLRDDNRNSLVRRSAEFALALDAEIVVMENARELIRGNFDHHYQCFRNVLEENG
                     YDVFGSVYLLSRFGLPQLRERAIVLAVKRKFELRTLDRLWEGWYVDERALTVRRAFDS
                     IAPNASATELFPDFSSESVRRRIEAIPSDGGSWMDLLKREDAERLLTDSMKRIVERNR
                     FGSYPDVYGRMAWDKPAPTIKR"
     gene            complement(1159..>1552)
                     /locus_tag="P5201_12775"
     CDS             complement(1159..>1552)
                     /locus_tag="P5201_12775"
                     /EC_number="4.2.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008710601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="dihydroxy-acid dehydratase"
                     /protein_id="HRV99463.1"
                     /translation="VVVIRYEGPKGGPGMREMFAAMKYLYGMGLAKKTALVTDGRFSG
                     TNNGCFVGHVSPEAAEGGPLAIVRDGDRITIDIPGGSLRIDLTDEEIAERLRAWKRPE
                     PTVKRGFLATYARLAGSAAEGAVMKLPE"
BASE COUNT          296 a          500 c          456 g          300 t
ORIGIN      
        1 ccctctttat cgtgggcgcc ggtttgtccc acgccatccg tccgtacacg tcggggtagg
       61 agccgaaacg gttccgttcg acgatacgtt tcatggaatc ggtcagaagc cgctccgcat
      121 cctcccgttt cagcagatcc atccatgagc cgccatcact cggaatcgcc tcgattcttc
      181 tgcgaaccga ctcggaggaa aaatcgggga acagctccgt cgcggaagcg ttcggggcga
      241 tggagtcgaa cgcgcggcga accgtgagcg ctcgttcgtc gacgtaccac ccctcccaga
      301 gcctgtccag agtgcgaagt tcaaattttc gttttaccgc caggacgatc gctctttcgc
      361 gtagctgcgg caagccgaaa cgcgaaagca gatagacgga accgaaaacg tcgtacccgt
      421 tctcttccag cacgttccgg aagcactggt agtgatgatc gaaatttccg cggataagct
      481 ctctggcgtt ctccatgacg acgatctcgg cgtcgagagc gagcgcgaac tccgccgacc
      541 ttcgaacgag actgttccgg ttgtcgtccc gaaggtgatt ctccggattc gcccggctga
      601 aaccggtaca gggggggcac gccgaaagga cggagacccg catcgtttca ggcaagccaa
      661 gtgcggtgcg caacaattcc ggaggcgtgt ccgaaagatc gagtttcgtc gggcacactc
      721 cggtattcaa acgataggcg ctgttacatt gaagagcgcc agcgccggaa ctcggctttc
      781 ccaactccgc gtcggccgcc gccgccagag aaaaaagagg atgtttatga aaaccgaaac
      841 tcattccccc gacgccggaa aagaggtcga cgacggcgaa tttctccgtt cccatcgata
      901 tccactcctt tatccacaaa ttttctcgac tcgatgagaa gacctacgcc cgccccctat
      961 cggtttcgga cggcgcgacg gggacgtttc actgaatttc cgccatattc gaagtgtacg
     1021 ccgaagcatc ttctttgtca aaacgcgtaa ccgccgccct gcgacggcga atcgccgcgc
     1081 gaaggccgga gtgctcgacg ctgaatcgcg gctctgccgc cgctatccct cttttcctcc
     1141 agccgcgagc gacgtggttc actccggcag cttcatgacg gcgccctcgg cggcggagcc
     1201 ggccagtctg gcgtaggtgg ccaggaagcc tctcttcacc gtcggctcgg ggcgcttcca
     1261 ggcgcgcagg cgctcggcga tctcctcgtc ggtcaggtcg atgcgcagcg agccgcccgg
     1321 gatgtcgatg gtgatccggt cgccgtccct gacgatcgcg agcggacctc cctccgccgc
     1381 ttcgggggag acgtgcccga cgaagcagcc gttgttggtg ccggagaatc gcccgtcggt
     1441 gacgagcgcg gtcttcttcg ccagccccat gccgtagagg tatttcatgg cggcgaacat
     1501 ctcgcgcatt ccggggccgc ccttcggccc ctcgtagcgg atgaccacga cg
//
LOCUS       DAOTIL010000741         1551 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminobacteriaceae bacterium isolate AMR_MDS_3026
            NODE_95793_length_1551_cov_3.691845, whole genome shotgun sequence.
ACCESSION   DAOTIL010000741 DAOTIL010000000
VERSION     DAOTIL010000741.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868050
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminobacteriaceae bacterium (wastewater metagenome)
  ORGANISM  Aminobacteriaceae bacterium
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae.
REFERENCE   1  (bases 1 to 1551)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1551)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:19:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,224
            CDSs (total)                      :: 3,198
            Genes (coding)                    :: 3,184
            CDSs (with protein)               :: 3,184
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1551
                     /organism="Aminobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_95793_length_1551_cov_3.691845"
                     /isolate="AMR_MDS_3026"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:3034609"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..805)
                     /locus_tag="P5201_12780"
     CDS             complement(<1..805)
                     /locus_tag="P5201_12780"
                     /inference="COORDINATES: protein motif:HMM:NF012927.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH oxidase"
                     /protein_id="HRV99464.1"
                     /translation="MKAYEPFHIKDLELKNRIVMPPMCMYSAPSSRVAPFHHLHYGAR
                     ALGGAGLIIIEATAVLPEGRLSDNCLGLWDDEQIAGMTELTRHIHSLGAKIGVQLNHA
                     GRKCGAAAETIYAPSAVNYSDDGKYKDPVEMNPYEIARVTQAFAAAAGRALRAGFDVV
                     EIHGAHGYLINQFLSPLSNRRRDGYGVNFEGRSRFLQEIARAVRTRWPEEKPLFLRIS
                     AEDYVEGGMTTGQMVRIVDRVTKEVDVVHVSSGGVTPVAPPVHPGYQIPF"
     gene            complement(1155..>1551)
                     /locus_tag="P5201_12785"
     CDS             complement(1155..>1551)
                     /locus_tag="P5201_12785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013048705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hydroxymethylglutaryl-CoA lyase"
                     /protein_id="HRV99465.1"
                     /translation="DGVTLCDTIGTTDPKYLANTLTEMRTRYGDYPFALHLHDTHGMA
                     LANMLTALRMGFSSFDAAAGGLGGCPFAPGAAGNAATEDMANMAERMGIATGIDIPKL
                     LAVVRSMKADYGLTTYSHLAAAEKSCCSC"
BASE COUNT          299 a          451 c          476 g          325 t
ORIGIN      
        1 cgaagggaat ctgatatccc ggatgcacag ggggagccac cggggttacc cctccggagc
       61 tgacgtggac gacgtccact tccttggtga ctctgtcgac gatacggacc atctgtcctg
      121 tggtcatacc tccctcgacg tagtcctccg ccgaaatccg gaggaagagc ggtttctcct
      181 cgggccagcg cgtccgtacc gccctcgcga tctcctggag aaagcgggac cggccctcga
      241 agtttacgcc gtagccgtca cgtctgcggt tgctcaacgg agagaggaac tggttgatca
      301 ggtagccgtg tgctccgtgg atctccacga cgtcgaatcc cgcccggagc gctcgccccg
      361 ccgctgcggc gaaggcttgg gtgacgcgcg caatctcgta ggggttcatc tcgacgggat
      421 ctttatactt cccgtcgtcg ctgtagttga cggcgcttgg cgcatagatg gtttcagccg
      481 ccgcgccgca cttccggccg gcatggttga gctgcacgcc gattttcgcc cccaggctgt
      541 ggatgtggcg cgtcagttcg gtcatgcctg ctatctgctc gtcgtcccaa agcccgaggc
      601 agttgtcgga gagacgtcct tcggggagga cggcggtcgc ctcgatgatg atcagcccgg
      661 ctcccccgag ggcgcgcgcg ccgtaatgga gatgatggaa aggtgcgacg cgggaagagg
      721 gcgcgctgta catgcacatg gggggcatga ctatacggtt tttcaattca agatctttga
      781 tatggaaagg ttcataggct ttcaatcaga acatgcctcc ttcaagagat acgtaaaata
      841 ccggaatctg cgccggtaga gaaagagtat cacgaaaagg cgtacatttc cacgtccgct
      901 ttgaaaccgg aaagaggaag aagacgcccc cgtggaacgg cagccgggtt ttgtagcaac
      961 tccccgagtt acgctccctc cggaacgagt gtgaagaaaa tgatggggca cgaaatcgaa
     1021 agccactcgt tgagagattc acgatgtgtt catgagactc tggctttctt gtgcggtaca
     1081 gcgtactcca taacaaggcc ccccttcggc aagcaactct gcagaagggg ggccttgttc
     1141 tttgccgaat cgtatcagca gctacagcag gatttttccg ccgccgcgag gtggctgtac
     1201 gtcgtcaacc cgtagtcggc cttcatgctg cgtacgacgg cgagcagctt tggaatatcg
     1261 atgcctgttg caattcccat gcgttccgcc atattcgcca tgtcctccgt ggcggcgttt
     1321 ccggctgcgc ccggcgcgaa ggggcagcct ccgaggccgc ccgccgccgc atcgaatgag
     1381 gagaatccca tccggagcgc ggtcagcatg ttcgcaagcg ccatcccgtg cgtatcgtga
     1441 agatggaggg cgaacgggta gtctccgtat ctggtccgca tctccgtaag cgtgttcgca
     1501 agatatttcg gatccgtggt gccgatcgtg tcgcagagcg tcacgccgtc g
//
LOCUS       DAOTIL010000742         1551 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminobacteriaceae bacterium isolate AMR_MDS_3026
            NODE_95813_length_1551_cov_3.070856, whole genome shotgun sequence.
ACCESSION   DAOTIL010000742 DAOTIL010000000
VERSION     DAOTIL010000742.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868050
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminobacteriaceae bacterium (wastewater metagenome)
  ORGANISM  Aminobacteriaceae bacterium
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae.
REFERENCE   1  (bases 1 to 1551)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1551)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:19:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,224
            CDSs (total)                      :: 3,198
            Genes (coding)                    :: 3,184
            CDSs (with protein)               :: 3,184
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1551
                     /organism="Aminobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_95813_length_1551_cov_3.070856"
                     /isolate="AMR_MDS_3026"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:3034609"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>1551
                     /locus_tag="P5201_12790"
     CDS             <1..>1551
                     /locus_tag="P5201_12790"
                     /EC_number="4.2.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012868776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="aconitate hydratase"
                     /protein_id="HRV99466.1"
                     /translation="DHNYLQDVAAKYGLLFSRPGNGICHQVHLERFGVPGKTLLGSDS
                     HTPTGGALGMLAMGAGGLDVALAMAGVPFSIPRPKVLKVELKGRLRPWVAAKDVILEV
                     LRVLTVKGGVGKILEYCGDGVSTLTVPERGTITNMGAELGATTSIFPSDEQTRRFLEA
                     QGRGTDFRPLAADGDAEYDETLVIDLSTLEPLIALPHSPDKVVPVREVAGMPVDQCFI
                     GSCTNSSYLDLARTAEILRGRRVAERTSLCIAPGSRQTLLMITKNGILTDFITAGARL
                     LESACGPCIGIGQAPCSGGVSLRTSNRNFEGRSGTKDALVHLVSAETAAVSALGGVIT
                     DPRTLGEAVEVRLPEKYIVDDSMVLLPLEDGSSVEIRRGPNIKPLPKFLPMADEIQGE
                     VLLKMGDNITTDHILPAGSRIMSLRSNLPAISEYCFNIVDPSFPERARKAGGGILVAG
                     ENYGQGSSREHAALSPLFLGVKAIITVSYARIHRQNLVNAGILPLLFEDRADLEKIAQ
                     GDRLELVN"
BASE COUNT          270 a          485 c          526 g          270 t
ORIGIN      
        1 acgaccacaa ctacctgcag gacgtcgcgg cgaaatacgg gctgctcttc tccaggccgg
       61 ggaatggcat ctgccatcag gtgcatcttg aacggttcgg cgttcccggc aagacgctcc
      121 tcggctccga cagccacact cccactgggg gcgccctggg gatgctcgcc atgggcgcgg
      181 gcgggctgga cgtggccctt gcgatggcgg gcgttccgtt ctccatcccc cggccgaagg
      241 tgctcaaggt ggagttgaag gggcgtctcc gtccgtgggt tgcggcgaag gacgtcattc
      301 tggaggtgct ccgcgttctc accgtaaagg ggggcgtcgg aaaaatcctt gagtattgcg
      361 gcgatggtgt ttcgacgctc actgtcccgg agcgcgggac gatcacgaac atgggggcgg
      421 agctcggcgc gacgacgtcc atcttcccga gcgacgagca gaccaggcgt ttcctcgagg
      481 cgcaggggag aggaacggac ttcaggcccc ttgccgccga tggagacgcg gagtacgacg
      541 agacgctcgt catcgacctg tcgacgctcg aaccgctcat cgcgctgccg cactcgcccg
      601 acaaggtggt cccggtgcgc gaggtcgcgg gcatgcccgt cgaccagtgc ttcatcggaa
      661 gctgtacgaa ctcttcctac ctcgacctcg cgcggactgc ggagattctg cgcggacggc
      721 gcgtcgcgga gcgcaccagc ctctgcatcg ctccaggctc gcgccagacg ctgttgatga
      781 ttacgaagaa cggtattctg acggacttca taaccgcggg cgcgcgcctg ctggagagcg
      841 catgcggccc gtgcatcggc atcggccagg cgccgtgcag cggcggcgtg tcgctgcgga
      901 cgtccaaccg gaatttcgag gggcgctccg gcacgaagga cgcgctggta catctggtga
      961 gcgcggagac ggccgcggtt tcggcgctcg ggggtgtgat caccgatccg aggacgctcg
     1021 gcgaggccgt cgaggtgcgg ctccccgaga agtatatcgt cgacgattcg atggtgctcc
     1081 ttcctctgga ggacggcagt tccgtcgaga tccgcagggg gccgaacatc aagccgcttc
     1141 ccaagttcct ccccatggcc gatgagatac agggcgaggt gctgctgaag atgggggaca
     1201 acatcacgac ggaccacatc cttcccgccg gaagccggat tatgtcgctg cgctcgaacc
     1261 tccccgccat ctccgagtac tgcttcaaca tcgtcgatcc ctccttcccg gagcgggcgc
     1321 gcaaggccgg aggaggaatc ctcgtcgcgg gggagaacta cgggcagggg tcgagcaggg
     1381 agcatgcggc cctctcgccg ctctttctcg gcgtcaaggc gatcatcacc gtctcgtacg
     1441 caaggatcca caggcagaac ctggtgaacg cggggattct tccgcttctc ttcgaagaca
     1501 gggccgatct tgagaagatc gcgcaagggg atcggctgga gctggtaaat c
//
LOCUS       DAOTIL010000743         1550 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminobacteriaceae bacterium isolate AMR_MDS_3026
            NODE_95912_length_1550_cov_3.257525, whole genome shotgun sequence.
ACCESSION   DAOTIL010000743 DAOTIL010000000
VERSION     DAOTIL010000743.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868050
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminobacteriaceae bacterium (wastewater metagenome)
  ORGANISM  Aminobacteriaceae bacterium
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae.
REFERENCE   1  (bases 1 to 1550)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1550)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:19:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,224
            CDSs (total)                      :: 3,198
            Genes (coding)                    :: 3,184
            CDSs (with protein)               :: 3,184
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1550
                     /organism="Aminobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_95912_length_1550_cov_3.257525"
                     /isolate="AMR_MDS_3026"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:3034609"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1521)
                     /gene="carB"
                     /locus_tag="P5201_12795"
     CDS             complement(<1..1521)
                     /gene="carB"
                     /locus_tag="P5201_12795"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: protein motif:HMM:NF003671.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyl-phosphate synthase large subunit"
                     /protein_id="HRV99467.1"
                     /translation="MPARNDLKKILVLGSGAIVIGQACEFDYSGVQAVKALKEEGYKV
                     VLANPNPATVMTTPGIADAIYLEPLKLPWVEEIIRCERPDALLPTMGGQTALNLALEL
                     ADEGILERYGVEIIGASVRSIRLAEDRGAFKDAVRSIGLDTPRSAVVRSAAEAFAFAE
                     ETPLPLIIRPSFTLGGLGGSIASTRKQLGIQVERALAESPVASALLEESLIGWKEFEM
                     EVMRDRRDNAVVVCSIENIDPMGVHTGDSVTVAPIQTLSDRAYQEMRTASLNILRVVG
                     VDCGGSNVQFAVHPESGRMVVIEMNPRVSRSSALASKATGFPIARAAAKLAVGYTLDE
                     IPNEITGKTTSCFEPALDYVAVKIPRFETEKFPAEYECLGTQMKSVGEALALGRTFME
                     ALNKAIRAAEAGYDGLQELEIGYDGLDRMLAPLHPRKLFAAYTVLCREGERALPLLAR
                     KTGIDPWFLYQMAEQADFEEGLTEGVLNASAVLKAKRFGLSDMRIASRSIPLHAAIA"
BASE COUNT          280 a          504 c          466 g          300 t
ORIGIN      
        1 ggcgatcgcg gcgtggagcg ggatcgagcg cgaggcgatg cgcatgtcgg agagaccgaa
       61 gcgtttggcc ttcagcaccg cgcttgcgtt cagaacccct tccgtcagcc cctcctcgaa
      121 atcggcctgt tcagccatct ggtacaggaa ccacggatca atgcctgtct ttcgcgccaa
      181 caacggaagc gctcgttctc cctcgcgaca gagtacagta taggcggcga agagttttct
      241 cggatgcagc ggcgcgagca tgcggtccaa tccgtcatag ccgatctcca gctcctgcag
      301 gccgtcgtat cccgcctctg cggcccgtat ggccttgttc agcgcctcca tgaacgtccg
      361 gccgagggcg agcgcctcgc cgacggactt catctgcgtc ccgaggcatt cgtactccgc
      421 ggggaacttt tccgtttcga agcgggggat cttcaccgcg acgtagtcga gcgcaggttc
      481 gaagcagctc gtcgtcttgc ccgtaatctc attcggtatc tcgtcgaggg tatagccgac
      541 tgcgagtttg gccgccgcgc gggcgatcgg gaagcccgtc gctttcgatg ccagcgcgga
      601 agagcggctc acgcgggggt tcatctcgat gacaaccatg cgccccgact caggatgcac
      661 ggcgaactgc acgttggaac cgccgcagtc cacgccgacg actctgagaa tgttcagcga
      721 cgccgtgcgc atctcctggt acgctctgtc gctgagcgtc tggatcgggg cgacggtcac
      781 gctgtccccc gtgtgcactc ccataggatc gatgttttca atggaacaga cgaccacggc
      841 gttgtccctg cggtcgcgca taacctccat ctcgaactcc ttccatccta tgagcgactc
      901 ctcaagaagc gcggaagcaa ccgggctctc cgcaagagcg cgctctactt gaatccccag
      961 ctgtttccgc gtggaggcga tggaacctcc caggccgccc agcgtaaacg acggccgtat
     1021 gatcagaggg agcggcgtct cttcggcgaa ggcgaacgct tccgcagcgc tgcgaacaac
     1081 agcggaacgc ggggtgtcca gtccgatcga gcgaacggcg tctttgaacg cccctctgtc
     1141 ctcggcaagg cgtattgagc ggacagacgc gccgatgatc tctacgccgt accgctccaa
     1201 aattccttcg tcggcgagtt caagggcaag attcagcgca gtctgccctc ccatggtcgg
     1261 caagagcgcg tcggggcgct cgcagcggat gatctcctcc acccacggaa gcttcaacgg
     1321 ttcgaggtag atggcgtcgg cgattccggg agtggtcatc accgtggcag gattggggtt
     1381 ggcgagcacc accttgtacc cctcttcctt cagcgctttg accgcctgca cccccgagta
     1441 gtcgaactcg cacgcctgcc cgatgacgat ggcgcccgac ccgagaacga ggatcttctt
     1501 cagatcgttt cgagcaggca cgaggatccc tcctttctcc tggaggcgat
//
LOCUS       DAOTIL010000744         1547 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminobacteriaceae bacterium isolate AMR_MDS_3026
            NODE_96210_length_1547_cov_2.235255, whole genome shotgun sequence.
ACCESSION   DAOTIL010000744 DAOTIL010000000
VERSION     DAOTIL010000744.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868050
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminobacteriaceae bacterium (wastewater metagenome)
  ORGANISM  Aminobacteriaceae bacterium
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae.
REFERENCE   1  (bases 1 to 1547)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1547)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:19:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,224
            CDSs (total)                      :: 3,198
            Genes (coding)                    :: 3,184
            CDSs (with protein)               :: 3,184
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1547
                     /organism="Aminobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_96210_length_1547_cov_2.235255"
                     /isolate="AMR_MDS_3026"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:3034609"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1179
                     /locus_tag="P5201_12800"
     CDS             <1..1179
                     /locus_tag="P5201_12800"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0052621 - diguanylate cyclase activity
                     [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor domain-containing diguanylate cyclase"
                     /protein_id="HRV99468.1"
                     /translation="RTVLTSLTASAFFGQVTFLLLIRTPSNLRDITRTSAFVMISYAV
                     LSLARALLLIFSPLETNEFFEAGGLTSVALLCYVGLSVCWLVAIILMIPRRLLEEVSV
                     QEEKFAKIFRSSPGAIMLTRKADGRIFDVNGGFLEMTGYAFEEIVGATVFATKLWGDD
                     KEYADIFSALSRDQGVRGIESVLRRKSGENMSVLFSSDVVVIDGEQCMLSTFSDITEQ
                     SRLRRQLHEMATHDPLTGLPNRRLFDERATDVFARATRQKRKFAIMSFDIDHFKDIND
                     TYGHAVGDEVLRQAAVRLKESLRITDTVARLGGDEFVALIEEVRGRSDASRVAFKLLE
                     LFRRPFIVEGHEVLIRVSIGVALYPENGETVEVLLKKSDDALYTAKEMGRDTLKMAED
                     "
     gene            1273..>1547
                     /locus_tag="P5201_12805"
     CDS             1273..>1547
                     /locus_tag="P5201_12805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013047549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lyase family protein"
                     /protein_id="HRV99469.1"
                     /translation="MDQGFRMEKDSLGEVQVSASKLWGAQTQRSLQNFRIGAEKMPEE
                     IIFAFALLKKAAARTNARLDLLDEVRMRAICGAADEILERKLDDQFPL"
BASE COUNT          365 a          361 c          453 g          368 t
ORIGIN      
        1 cgtactgtgc tgacgtcgct cacagcctct gcgttttttg gccaggttac tttccttctg
       61 ctgatacgaa cgccttcgaa tctgcgcgat attaccagga cgagcgcgtt cgtcatgatt
      121 tcttatgcag tgttgagcct tgcccgggcg cttctcctca ttttttctcc cctggaaacg
      181 aacgaattct tcgaagctgg agggctcact tcagttgcgc tgctctgtta tgtcggtctg
      241 agcgtctgtt ggctcgtcgc gattatcctg atgattcccc gccgcctgct cgaagaggtc
      301 agtgtccaag aggaaaagtt cgcgaagata ttccgttcat cccctggcgc gatcatgctt
      361 acgagaaaag cggacgggag aattttcgac gtgaacggcg gttttcttga gatgacagga
      421 tatgcgttcg aagagattgt aggcgccact gtctttgcaa ccaaactgtg gggagatgac
      481 aaggaatatg ccgatatttt ctctgccttg tcaagagacc agggcgtacg ggggatcgag
      541 agtgtcctcc ggagaaaatc gggagagaac atgagtgttc ttttctcgtc ggatgttgtt
      601 gtgatcgacg gcgagcagtg tatgctgtcg actttctcgg atataacgga gcagagccgg
      661 ctgcggcggc agctgcacga aatggccacg catgatcccc tgacaggact gcccaatcgc
      721 aggctcttcg atgaacgggc aaccgacgtc ttcgcacgtg cgacgaggca aaaacgcaag
      781 tttgccataa tgtccttcga catcgaccac ttcaaggaca ttaacgatac ctacggacac
      841 gcagtcggag atgaagtgtt acggcaggca gctgttcgtc tgaaagaatc tctgcgtata
      901 accgataccg ttgcccgcct tggcggcgac gagttcgtcg ctttgataga ggaggttcgt
      961 ggacgaagcg acgcctcaag agtggccttc aaactcctgg agcttttccg aaggccgttc
     1021 attgtggagg ggcatgaggt actcatcagg gtgagtatag gcgttgcgct ttatccggaa
     1081 aatggcgaaa ctgtcgaagt ccttttaaag aagtccgacg atgcgctcta caccgcaaaa
     1141 gagatggggc gggacacttt gaaaatggcg gaggactgag aaaacaagga tgcttttgag
     1201 ttctttttgg tgaacggtac aatgggcgcg aaacgaaaaa agagcgtgaa gccgcgcaag
     1261 gagggtatgc ttatggatca ggggtttcga atggagaagg actctctggg agaagtgcag
     1321 gtatctgctt caaaactgtg gggcgctcag acccagagaa gtttgcagaa ctttagaata
     1381 ggcgcggaaa aaatgcccga ggagatcatt tttgcatttg cactgttgaa aaaagccgcc
     1441 gcgagaacaa acgcccgcct tgatttgctt gacgaagttc gtatgcgggc gatttgcgga
     1501 gcggccgacg aaatcctgga gaggaagctc gacgatcagt ttcccct
//
LOCUS       DAOTIL010000745         1543 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminobacteriaceae bacterium isolate AMR_MDS_3026
            NODE_96566_length_1543_cov_1.942876, whole genome shotgun sequence.
ACCESSION   DAOTIL010000745 DAOTIL010000000
VERSION     DAOTIL010000745.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868050
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminobacteriaceae bacterium (wastewater metagenome)
  ORGANISM  Aminobacteriaceae bacterium
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae.
REFERENCE   1  (bases 1 to 1543)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1543)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:19:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,224
            CDSs (total)                      :: 3,198
            Genes (coding)                    :: 3,184
            CDSs (with protein)               :: 3,184
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1543
                     /organism="Aminobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_96566_length_1543_cov_1.942876"
                     /isolate="AMR_MDS_3026"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:3034609"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..660)
                     /locus_tag="P5201_12810"
     CDS             complement(<1..660)
                     /locus_tag="P5201_12810"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRV99470.1"
                     /translation="MLTKRFLPAFFILCVLLVGCAFAASSEQPKTNLPFTVIAADVDE
                     VIIYEGDDEEVQPLYAVLMGVGEERAIKGLVSGDNVTTQRIRWSIDNWGVARIVGQEN
                     EGDVIEANEIAVEAWGNGTATIIATSVQDPTISEFCTVYVQNSPVPVEEVTIDKEEIG
                     ILSGYFGELTASVLPPQADFRHISWHSSNPEIVAIDPTMRTGNRTRLLTAGLHTGTAI
                     IS"
     gene            complement(910..>1543)
                     /locus_tag="P5201_12815"
     CDS             complement(910..>1543)
                     /locus_tag="P5201_12815"
                     /inference="COORDINATES: protein motif:HMM:NF012382.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HRV99471.1"
                     /translation="IMREQAQAIAEVAKKHDLFVISDEIYEAFVFSGKHIPMASLPGM
                     KERTMTIGGFSKSHCMTGWRIGYVMAPAALLKVMVQLSVVQTFGVNVLSQMGGIEALK
                     TQDAKMRERTAEYEKRVRYSADRLNAMPGVSCPEPKGAFYLFPDISGTGMTDEEFVWW
                     LLETAKVATIPGTAFGESGAGHIRIACTLPMEGLKKAFDRMETALKNRKN"
BASE COUNT          309 a          453 c          401 g          380 t
ORIGIN      
        1 ggagataatt gctgtacctg tgtggagtcc tgcagtcaaa aggcgtgtgc ggttacctgt
       61 tcgcattgta gggtctatag ctacaatttc cggattgctc gaatgccagg aaatatgacg
      121 aaaatctgct tgaggcggca agacgctcgc tgtcagttct ccgaagtagc cgctgaggat
      181 accgatctct tccttgtcga tggtgacttc ctctaccggg actggcgaat tttgtacgta
      241 cacggtgcaa aattcggaaa tagtgggatc ctgaacagaa gttgcaatta tggtggcagt
      301 tccgtttccc cacgcttcca ccgcgatctc gtttgcttcg atcacgtcgc cttcgttctc
      361 ctgtccgaca atccttgcga ctccccagtt atcgatagac cagcgtatgc gctgtgtcgt
      421 tacattgtct cccgatacaa gccccttgat cgcccgctct tccccgacgc ccatcagaac
      481 agcgtagaga ggttgtactt cttcgtcgtc tccctcgtag atgataactt cgtcaacatc
      541 ggcggcaata actgtgaaag ggaggtttgt ctttggctgt tccgaggaag ccgcgaatgc
      601 acagccgacc aacagcacgc agaggataaa aaacgcagga agaaaccttt ttgtcagcat
      661 acttgtccac accgtcctta tgctcgaata aaaacatcgc caccaattat agcacggcgt
      721 tctcaaaggg gagcgacttt tctgagcatt tggcgtcgga ttgaaggatt tttttctcac
      781 tttctttcac gtccttggca atccgttctt tctgattgga cgcttctcat cccggcggaa
      841 ttgtttcagc acagaaaaaa ggcgggagca tgtacggccc ccgcccgtgc gtacgcttct
      901 tcccttgctt tagttcttcc gattcttcag cgcggtttcc atcctgtcga acgccttctt
      961 cagcccctcc atcgggaggg tacaggcgat acggatgtgc cccgcgccgc tttctccgaa
     1021 ggctgttccg ggaatcgttg ctaccttcgc ggtctcaagc agccaccaga cgaactcctc
     1081 gtcggtcatt ccggtccccg agatgtcggg gaagaggtag aaagcgccct tcggctccgg
     1141 gcagctcacg cccggcatcg cattcaggcg gtcggcgctg tagcggacgc gcttctcgta
     1201 ctcggcggta cgttcgcgca tcttcgcatc ctgcgtcttc agcgcttcga tccctcccat
     1261 ctgggagaga acgttgacgc cgaacgtctg gaccacggag agctgtacca tcactttcag
     1321 cagagccgcc ggggccatca catagccgat gcgccagccg gtcatgcagt ggctcttcga
     1381 gaaaccgccg atggtcatcg tccgctcctt catgcccggg agcgacgcca tggggatgtg
     1441 tttccccgag aagacgaagg cctcgtagat ctcgtcggag atcacaaaga gatcgtgctt
     1501 cttcgccacc tcggcgatgg cctgcgcctg ttcacgcata atc
//
LOCUS       DAOTIL010000746         1541 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminobacteriaceae bacterium isolate AMR_MDS_3026
            NODE_96702_length_1541_cov_2.815612, whole genome shotgun sequence.
ACCESSION   DAOTIL010000746 DAOTIL010000000
VERSION     DAOTIL010000746.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868050
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminobacteriaceae bacterium (wastewater metagenome)
  ORGANISM  Aminobacteriaceae bacterium
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae.
REFERENCE   1  (bases 1 to 1541)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1541)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:19:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,224
            CDSs (total)                      :: 3,198
            Genes (coding)                    :: 3,184
            CDSs (with protein)               :: 3,184
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1541
                     /organism="Aminobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_96702_length_1541_cov_2.815612"
                     /isolate="AMR_MDS_3026"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:3034609"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..377
                     /locus_tag="P5201_12820"
     CDS             <1..377
                     /locus_tag="P5201_12820"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRV99472.1"
                     /translation="GKRCFVTKARRDFVFVKDLAKAVLRSCDGTGSGTYHFSSGKDIA
                     IKELYDAVVHAMKLPEYPEPEVRDLGPDDAPSILLDPSRLFRDFGEFEFSPVSVTVGE
                     AVAYYEKFGVSGGYTHLKHEEK"
     gene            393..1337
                     /locus_tag="P5201_12825"
     CDS             393..1337
                     /locus_tag="P5201_12825"
                     /inference="COORDINATES: protein motif:HMM:NF027681.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HRV99473.1"
                     /translation="MRILVTGGAGCLGSNLIEHWLPAGHEILAIDNFATGKREVLPDT
                     DGLTVMEGSVADQELIRSAFDSFEPEFVAHSAASYKNPIDWREDVNTNVSGAVFLAQE
                     AMRCGVKKIVNFQTALCYGRPQTVPVPVDHSTLPFTSYGISKTAGEAYLLQSGLPVIS
                     LRLANTCGPRLAIGPIPTFYKRLKAGQGCFCSETIRDFLDMSDFLSLVDMLLENTIVS
                     SGIFNVSTGKGYSIKDVYDAVGKYLEIVPPEVPIVPPSADDVPVMVLDPSETERVLGW
                     KARISFEETINRQLKWYDAHGVNDVFSHLVHPKEEGVV"
     gene            1337..>1541
                     /locus_tag="P5201_12830"
     CDS             1337..>1541
                     /locus_tag="P5201_12830"
                     /inference="COORDINATES: protein motif:HMM:NF013530.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HRV99474.1"
                     /translation="MSLGFEKSKILVVGGAGFVGSNLCHLLLTDKKVEELWIVDNLLS
                     SDICNVPEDPRVHFLFGSICDDRI"
BASE COUNT          361 a          368 c          422 g          390 t
ORIGIN      
        1 aggggaagcg atgttttgtc acaaaggcca ggcgcgattt tgtcttcgtc aaggatcttg
       61 cgaaggcggt actccgctcc tgcgatggta cagggagcgg gacgtatcat ttttcatcgg
      121 gtaaggatat agccattaaa gaactttatg atgctgttgt acatgccatg aaactacccg
      181 aatatcccga accagaagta cgcgatctgg gtcctgacga cgctccttcg atactgcttg
      241 acccctcccg cttgtttcgt gatttcggag agttcgaatt ttctccggtt tccgtaactg
      301 tgggagaagc ggtggcttac tatgaaaaat ttggtgtttc gggcggctat acacacctga
      361 aacacgaaga aaaatagtga gaactgaacg aaatgcgtat tctagttacc ggaggcgccg
      421 gatgtctcgg ttcgaacctc atcgaacatt ggctgccggc gggacatgaa attctagcga
      481 tagataactt tgcaacggga aagagggaag tgctcccaga tactgacgga ctgaccgtca
      541 tggagggaag cgtcgcagac caggagctga tacggtctgc gttcgattcg ttcgaacccg
      601 agtttgtcgc ccacagtgcc gcatcataca aaaacccgat agattggcgg gaagatgtca
      661 acacgaacgt ttccggggct gtatttttgg ctcaggaagc aatgcggtgc ggagtgaaaa
      721 aaatcgtcaa tttccagaca gccctttgct acggacggcc ccaaacagtt cccgttccag
      781 tggatcattc gaccttgcct tttacaagct acggtatttc caagactgcc ggagaggctt
      841 acctgctgca atccgggctt cccgtaattt cacttcggct ggccaacact tgcggcccgc
      901 gacttgcaat tggccctatc cctacctttt acaaacgcct taaggctgga cagggttgtt
      961 tttgcagcga gactataagg gattttctcg acatgagcga cttcctctcc cttgttgata
     1021 tgctgctgga aaacaccatt gtatcgagtg ggattttcaa tgtctcgacg gggaaaggtt
     1081 actccataaa ggatgtttat gatgcagtag gtaagtatct ggaaatagtt ccgccggaag
     1141 tccccatcgt tcctccttcc gctgatgatg ttcctgtgat ggtcctggat ccttcggaga
     1201 ccgagcgagt gctgggttgg aaggcgcgaa tctccttcga ggaaacgatc aaccggcagt
     1261 tgaagtggta cgatgcgcac ggtgtcaacg atgtcttcag tcacctggtg catccgaaag
     1321 aggagggtgt tgtctgatgt cgctcgggtt cgaaaagagt aagatcctgg tggtaggagg
     1381 ggcgggtttc gtcggaagca acctttgcca cctgttgctc acggataaaa aggtggagga
     1441 actctggatc gtcgacaatc ttctttcgtc cgacatatgc aacgtgcccg aagatccgag
     1501 agtgcatttt ctcttcggct cgatctgcga cgatcgcatt c
//
LOCUS       DAOTIL010000747         1538 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminobacteriaceae bacterium isolate AMR_MDS_3026
            NODE_96958_length_1538_cov_3.532030, whole genome shotgun sequence.
ACCESSION   DAOTIL010000747 DAOTIL010000000
VERSION     DAOTIL010000747.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868050
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminobacteriaceae bacterium (wastewater metagenome)
  ORGANISM  Aminobacteriaceae bacterium
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae.
REFERENCE   1  (bases 1 to 1538)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1538)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:19:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,224
            CDSs (total)                      :: 3,198
            Genes (coding)                    :: 3,184
            CDSs (with protein)               :: 3,184
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1538
                     /organism="Aminobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_96958_length_1538_cov_3.532030"
                     /isolate="AMR_MDS_3026"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:3034609"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(40..1065)
                     /locus_tag="P5201_12835"
     CDS             complement(40..1065)
                     /locus_tag="P5201_12835"
                     /inference="COORDINATES: protein motif:HMM:NF028468.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MASE3 domain-containing protein"
                     /protein_id="HRV99475.1"
                     /translation="MHWKRHLPVLAVLTALLWYGANHNYLLFHSIVEFADITIYLFAA
                     FLGVFASRMTPDPFLYGVSVVYFAMALVTAVHTLAFEGMGVFPGWTANHPTQLWMLMR
                     FIHGIGVPLTLLFSSRPQFIRFFTAGAMLSVFGGILLIAAGYFPDCFIPGEGLTLFKV
                     AGEYAVALLLVLSILYSLAYRRREARRYGYSFEISLACFIASGMAFTLYTDVYGFFNM
                     LGHILHGYGAYILLFGFVVKSAQTLMDRHFYDLNEQIRNMNRELENRVAQRTRELEDA
                     NALLASDIARRKEAEEHLRTAKEEAEEANRAKSAFLATMSQREASRSKRPSEGTKSAR
                     RSTAFSA"
     gene            1283..>1538
                     /locus_tag="P5201_12840"
     CDS             1283..>1538
                     /locus_tag="P5201_12840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014163997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HRV99476.1"
                     /translation="MSIYMNNGATTWPKPESVARAMYDFLTGGGANVARGSASKRDLK
                     SMDTVLSCREELVSLFGGWDDDPRYATFTANVTESINVVLK"
BASE COUNT          325 a          423 c          494 g          296 t
ORIGIN      
        1 gctcttccgg cttcagatcc tcgtccttca ggtgggccgt catgccgaga atgccgttga
       61 gcggcgtgcg gacttcgtcc cttcgctcgg acgcttgctc cgggaggctt cgcgctggct
      121 catcgtggcg aggaaggcgc tcttcgcccg gtttgcctcc tccgcttcct ccttggccgt
      181 gcggagatgc tcctcggcct ccttgcgccg ggcgatgtcc gaggcaagga gcgcgtttgc
      241 gtcctccagc tcgcgcgtcc gctgcgcgac acggttttcc agttcgcggt tcatattccg
      301 tatctgctcg ttcaagtcgt aaaaatgcct gtccatcagc gtttgggccg acttgacgac
      361 gaagccgaag agcagaatgt aggcgccgta gccgtggaga atatgcccca gcatattgaa
      421 gaagccgtag acgtccgtgt agagggtgaa ggccattccg cttgcgatga agcaggcgag
      481 cgaaatttcg aaggagtagc cgtatcgccg cgcctccctg cggcggtacg cgagcgaata
      541 caggatggaa agaacaagca gcagcgcgac ggcgtactcg cccgccacct tgaagagcgt
      601 gagtccttcg ccggggatga agcagtccgg gaagtatccc gccgcgatca gcagaatgcc
      661 gccgaagacc gagagcatcg cgcccgcggt gaaaaaacgg atgaactgag gacgcgagga
      721 gaagaggagc gtcagcggaa cgccgattcc gtgaatgaag cgcatcagca tccagagctg
      781 cgtggggtgg ttcgccgtcc accccgggaa gactcccatt ccctcgaagg cgagcgtgtg
      841 gacggccgtg acgagcgcca tggcgaagta gacgacgctg acgccgtaga ggaaaggatc
      901 cggcgtcatt cgcgaggcga agactccgag aaaggccgcg aagagataga tcgtgatgtc
      961 ggcgaactcc acgatcgagt gaaagagcag gtagttgtga ttcgctccgt accagagaag
     1021 cgctgtcagg acggcgagaa ccggcagatg acgtttccag tgcatagaaa atcctccttg
     1081 gtacggcaaa gaaggcaggg ggtatcttct gacactgtct ccaccgtttt ccagtgaaag
     1141 tatagcattg tttcccacga atcgctccgg gaacgcgccg ttctttaccc gttgcagccg
     1201 catctttccc ccgaagtctg gcgtttttgc agaaaagcag tacaatggtg catatatttt
     1261 ataataagga agggtgatcg ctatgtccat ttatatgaac aacggggcaa ctacgtggcc
     1321 gaagccggag agcgtggcgc gggcgatgta cgactttctg acgggagggg gggcgaacgt
     1381 cgcgcggggg agcgcctcga agcgggatct gaagagtatg gacaccgtcc tctcctgcag
     1441 ggaggagctt gtttcgctgt tcggcggatg ggacgacgat ccccgctacg ccacgttcac
     1501 ggcgaacgtg acggaatcca tcaacgtcgt cttgaagg
//
LOCUS       DAOTIL010000748         1537 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminobacteriaceae bacterium isolate AMR_MDS_3026
            NODE_97052_length_1537_cov_4.057355, whole genome shotgun sequence.
ACCESSION   DAOTIL010000748 DAOTIL010000000
VERSION     DAOTIL010000748.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868050
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminobacteriaceae bacterium (wastewater metagenome)
  ORGANISM  Aminobacteriaceae bacterium
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae.
REFERENCE   1  (bases 1 to 1537)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1537)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:19:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,224
            CDSs (total)                      :: 3,198
            Genes (coding)                    :: 3,184
            CDSs (with protein)               :: 3,184
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1537
                     /organism="Aminobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_97052_length_1537_cov_4.057355"
                     /isolate="AMR_MDS_3026"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:3034609"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            353..>1537
                     /locus_tag="P5201_12845"
     CDS             353..>1537
                     /locus_tag="P5201_12845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014807848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016866 - intramolecular transferase
                     activity [Evidence IEA];
                     GO_function: GO:0031419 - cobalamin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl-CoA mutase family protein"
                     /protein_id="HRV99477.1"
                     /translation="MNKEYTEAKARFEAGVMKTLQKNKERKETFSTGGGLPLERLYGP
                     DMTEGTDYVKEVGFPGEYPFTRGVQSTMFRGRFWTMRQYAGFASAEESNERYRYLLAS
                     GTTGLSVAFDLPTQIGYDSDDPMAQGECGKVGVAIDSLKDMEILFDKIPLDKVSTSMT
                     INAPASVLLAMYICVGEKQGVPMSALSGTIQNDILKEYIARGTYIFPPKPSMRLITDI
                     FEFCSQNVPKWNTISISGYHIREAGSTAAQEVAFTLADGIAYVEAARSKGLDPNVFGE
                     RLSFFFNAHNDFLEEVAKFRAARRMWAKIMKERFGVTNPKAQMLRFHTQTGGSTLTAQ
                     QPENNIVRVTMQALSAVLGGTQSLHTNSMDEALALPSEKSVMIALRTQQIIAYESGVT
                     NTV"
BASE COUNT          362 a          400 c          431 g          344 t
ORIGIN      
        1 gaaacagtcc agctcacggg tttttgtttg caaaaccgtg tgcgaatctt tgtattcgcg
       61 gacagtctcc cgtccgtaga gctgcttcga gcggcttcac tgacgggagg aagggagacg
      121 tttgttccaa acggcacatg gggacccttt ccggcgtatt cttcaggcgc tctcaggatc
      181 gtgctccctg ccgacgagac ctcctcggga tttccgagca ggtcgacgct ttctgtctat
      241 ctggacatcg gagttgttcc gacgagagat tgggtccctc tctggccgga tcttttgtga
      301 tcggtcggag ttgaaaagta cgcactatct tccaaaggag gagaggttga gcatgaacaa
      361 agagtataca gaagcgaaag cccgttttga agcgggagtc atgaaaactc ttcaaaaaaa
      421 caaagagcgg aaagagactt tttcaacggg gggaggtctt cctcttgaac gtctgtacgg
      481 acctgatatg acggagggga ccgattatgt gaaggaagtc ggcttccccg gggagtatcc
      541 ctttacacga ggcgtgcagt cgacgatgtt cagaggtaga ttctggacga tgcgccaata
      601 tgcggggttt gcttccgccg aggagtcgaa cgagagatac cgctatcttc ttgcttccgg
      661 cacgacgggg ctctcagttg ccttcgacct ccccacgcaa attggttacg actccgacga
      721 cccgatggca cagggagaat gcgggaaagt cggcgttgcc attgacagtt tgaaagacat
      781 ggaaattctt ttcgacaaga ttcctctcga caaggtatcg acgtcgatga cgatcaacgc
      841 tccggcaagc gttctactgg ccatgtacat ctgcgtggga gaaaagcagg gagttcctat
      901 gagcgctctt tccgggacca tccagaacga catactgaag gagtatattg cacgaggaac
      961 ctatatcttt cccccgaaac cctccatgcg cctcatcacc gatatttttg agttctgtag
     1021 ccagaacgtg ccgaagtgga ataccatttc catttccggg taccatatcc gggaggccgg
     1081 cagcacggcg gcgcaggaag tcgcgtttac gctcgcagat ggaatagcct atgttgaagc
     1141 ggcgcgttcg aaaggactgg acccgaatgt cttcggcgaa cgcctttcct tcttcttcaa
     1201 cgcgcacaac gatttcctcg aagaggttgc gaaattccgc gctgcacgcc gtatgtgggc
     1261 gaagattatg aaggagcgtt tcggcgtgac aaatccgaaa gcgcagatgc tccgtttcca
     1321 tacgcagacg ggcggcagca ctctgaccgc gcagcagccg gagaacaaca tcgttcgggt
     1381 gaccatgcaa gcgctctcgg ctgttcttgg aggaacgcag tctcttcaca ccaacagcat
     1441 ggacgaggcc ctcgcgcttc cgagcgagaa gagcgttatg atcgcactca gaactcaaca
     1501 gatcattgct tacgaatccg gagtcaccaa cacggtt
//
LOCUS       DAOTIL010000749         1537 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminobacteriaceae bacterium isolate AMR_MDS_3026
            NODE_97055_length_1537_cov_3.582996, whole genome shotgun sequence.
ACCESSION   DAOTIL010000749 DAOTIL010000000
VERSION     DAOTIL010000749.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868050
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminobacteriaceae bacterium (wastewater metagenome)
  ORGANISM  Aminobacteriaceae bacterium
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae.
REFERENCE   1  (bases 1 to 1537)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1537)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:19:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,224
            CDSs (total)                      :: 3,198
            Genes (coding)                    :: 3,184
            CDSs (with protein)               :: 3,184
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1537
                     /organism="Aminobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_97055_length_1537_cov_3.582996"
                     /isolate="AMR_MDS_3026"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:3034609"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..585)
                     /locus_tag="P5201_12850"
     CDS             complement(<1..585)
                     /locus_tag="P5201_12850"
                     /inference="COORDINATES: protein motif:HMM:NF012694.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system F family protein"
                     /protein_id="HRV99478.1"
                     /translation="MPSYRYSAYDTSGKLRKGIIDAPSSTRAGELLAEQNLVAVEIIE
                     GAAESVKKGKVFGLAYHSLFCKGLSSYLNRGVPLAEALRFLSRHSNDRRVSAACLHLH
                     ESVQGGKRLSAALEETGLFRDDLVRIVDSGERTSALSGVLDQVARQYAMEDKQRRKIR
                     SALTYPVAMSIIGIGVVVFLLTYVVPKLTELFSEM"
     gene            complement(589..>1537)
                     /locus_tag="P5201_12855"
     CDS             complement(589..>1537)
                     /locus_tag="P5201_12855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005658727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ATPase, T2SS/T4P/T4SS family"
                     /protein_id="HRV99479.1"
                     /translation="PQDGRIGITVGKRAVDIRVNSIPVQHGERIALRILDKGAGLLSL
                     EQLGMDATSREAMESLIQIPHGLILFTGPTGSGKTTTLYAILQALAKPAVNIITVEDP
                     IEYAVPGVGQIQVNEKAGVTFPSALRSILRQDPDIVMIGEMRDYETAHIGVQASLTGH
                     LVLSTLHTNDSISAVVRLIDMGIEPYLAAGSLLGAIAQRLVRTNCPECIEEFEAPSLF
                     RKEGIRKVMRGRGCPACMGTGFKGRVGLYEQFLLDDSLREAVASNLPQSRIRAEARKH
                     GFRTLWELGLDALREGRTSPEELLRVVSAGEFASDEGGD"
BASE COUNT          319 a          462 c          451 g          305 t
ORIGIN      
        1 catttccgaa aaaagttccg tcagcttcgg gactacgtag gtcaacagga aaacgaccac
       61 accgatgcca ataatgctca tggcaaccgg ataggtgaga gccgaacgga tcttgcgtcg
      121 ctgtttgtcc tccatcgcgt actggcgcgc tacctggtcg agaactccgc tcagggccga
      181 tgttcgttct cccgagtcga cgatgcgaac aaggtcgtcc cggaagagtc ctgtctcctc
      241 gagcgccgcg gagagacgtt ttcctccctg cacgctctcg tgcagatgga gacaggccgc
      301 cgagacacga cgatcattgg agtggcggct gaggaagcgt aaagcttccg cgagcggcac
      361 ccccctgttc agataagaag agagcccttt acagaaaagc gagtgatagg cgaggccgaa
      421 caccttgcct ttctttaccg attcggccgc cccttcgatg atctcaacgg ccaccaggtt
      481 ctgctctgcg aggagttcac ctgcgcgggt gctcgacggc gcgtcgataa tccccttgcg
      541 gagctttccc gaggtatcgt atgcagagta gcgataagaa ggcatctatc agtctcctcc
      601 ctcgtccgaa gcaaattcgc cggcggacac aacgcgaagc agctcttccg gagacgttct
      661 cccttcgcgg agcgcatcga gcccaagctc ccagagcgtc ctgaaaccgt gtttgcgggc
      721 ctcggcgcgt attctggact gcgggaggtt ggaggccact gcttccctga gactgtcgtc
      781 caggaggaac tgctcgtaga gaccgacacg ccccttaaaa ccggtaccca tacatgcggg
      841 gcagcctctc ccccgcatca ctttgcgtat gccttccttt cggaagagag agggcgcttc
      901 gaactcctca atgcactcag ggcagttcgt ccggacaagg cgctgcgcaa tggctccgag
      961 aagcgaccct gccgcgagat agggctcaat ccccatgtcg atcaaacgca cgacggccga
     1021 gatcgaatcg ttggtgtgga gcgtcgagag gacaagatgt cctgtcaggg acgcctgcac
     1081 accgatgtgc gccgtttcgt agtcgcgcat ttcgccgatc atcacaatat cggggtcctg
     1141 gcgcagaatc gaacgcaggg cggacgggaa ggtcaccccg gccttctcgt tcacctggat
     1201 ctgccccacg ccgggaacgg cgtattcgat agggtcttcg acggtgatga tgttcaccgc
     1261 cggcttcgcc agagcctgca agatagcgta gagcgtcgtc gtctttcccg atccggtagg
     1321 gccggtgaag aggataaggc cgtgcgggat ctgaataaga ctttccatcg cctctcgtga
     1381 cgtggcgtcc ataccgagct gctccagaga gagcaaacct gcccccttgt cgagtatacg
     1441 aagtgcaata cgttcaccgt gctggacggg gatagagttg acgcgaatgt ccacagcacg
     1501 tttgccgacg gtgatgccga tgcgtccatc ctgcgga
//
LOCUS       DAOTIL010000750         1535 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminobacteriaceae bacterium isolate AMR_MDS_3026
            NODE_97234_length_1535_cov_3.174324, whole genome shotgun sequence.
ACCESSION   DAOTIL010000750 DAOTIL010000000
VERSION     DAOTIL010000750.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868050
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminobacteriaceae bacterium (wastewater metagenome)
  ORGANISM  Aminobacteriaceae bacterium
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae.
REFERENCE   1  (bases 1 to 1535)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1535)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:19:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,224
            CDSs (total)                      :: 3,198
            Genes (coding)                    :: 3,184
            CDSs (with protein)               :: 3,184
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1535
                     /organism="Aminobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_97234_length_1535_cov_3.174324"
                     /isolate="AMR_MDS_3026"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:3034609"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1173)
                     /locus_tag="P5201_12860"
     CDS             complement(<1..1173)
                     /locus_tag="P5201_12860"
                     /inference="COORDINATES: protein motif:HMM:NF012700.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease MutS2"
                     /protein_id="HRV99480.1"
                     /translation="MEIKEDVSRILEIPKILNVFASSVRGELGLFALDRLKPQPSLRS
                     LLERIELFRSFMEFRDRNGEWPWNPKVVCVSFMLQGAKRSGMLSGEELLSVARLLTLA
                     NVTREILAKSREQYPAFEPPFRRFRDFSGEIQALGVLDDNGVLYDTATPELASIRQDV
                     DSLRRRIRRAAQSLLDTPSVSQMLQDRVLAYRNSHFVFLVRQEYVNRFPGTVVDRSGS
                     GNSIYMEPSVLVPLNNTLALRLRDQEEEEHRILRKLTSDILARERPILDAQDVLAEFD
                     LLYGGAEVLSKRRWKLPRIEEKTSFSLHQARHPLLGEKAVPVNIKCGEGFRSLVITGP
                     NTGGKTVVLKTAGVCIYLAWCGLPIPAEEDSRVGNIGAIFADIGDEQSIEQNLSTFS"
     gene            complement(1163..>1535)
                     /locus_tag="P5201_12865"
     CDS             complement(1163..>1535)
                     /locus_tag="P5201_12865"
                     /inference="COORDINATES: protein motif:HMM:NF014704.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="CvpA family protein"
                     /protein_id="HRV99481.1"
                     /translation="MALIYIAAVVGAALMCKMVRAFLKFTSLAFIDRILGAGAGLLKG
                     AALILFLYVGITTYSPIIPSEWMGESVVMRGAHAAWPVVQARMREWNIFPENFTLPEL
                     NLPSLFPQAGEETDGGSSDGN"
BASE COUNT          331 a          449 c          459 g          296 t
ORIGIN      
        1 gctgaaggtg gacaggttct gctcgatgct ctgttcgtcg ccgatgtccg cgaaaatggc
       61 gccgatgttc cccaccctgg agtcctcctc cgccggaatc ggaagaccgc accacgcgag
      121 gtaaatgcag acgcccgcgg ttttgaggac gaccgtcttt ccgcccgtat tcgggcctgt
      181 gatcacaagg cttcgaaatc cttcgccgca cttgatgttc acggggacgg ccttttcccc
      241 gaggagggga tgccgcgcct ggtgaagtga aaaagatgtc ttctcttcta tccgggggag
      301 cttccagcgg cgtttggaga gcacttcggc gccgccgtag aggaggtcga actcggcaag
      361 cacatcctgc gcgtcgagaa tcggacgttc ccgcgccagt atgtccgagg taagcttccg
      421 gagaatacga tgttcttcct cttcctggtc gcgcaacctg agggcgagcg tattgttgag
      481 cggcacgagc accgacggtt ccatatagat ggagttcccg gagcccgacc tgtcgacaac
      541 ggttcccgga aaccggttca cgtactcctg acgaacgagg aagacaaagt gactgtttct
      601 gtaggcgagc accctgtcct ggagcatctg cgagaccgag ggggtgtcga gaagcgactg
      661 ggcggctctg cggatccttc tgcgcaggga atcgacatcc tggcgtatag aggccagctc
      721 aggggtcgcg gtatcgtaca gtacaccgtt gtcgtcgagg acgccgagcg cctgtatctc
      781 tcccgagaaa tccctgaagc ggcggaaagg gggttcaaac gcagggtact gctcacgcga
      841 cttcgcaaga atttccctcg taacattggc aagcgtcaaa agccgggcga cggaaagcaa
      901 ctcctccccg gaaagcatac ccgaacgctt cgcgccctga agcatgaaag aaacacagac
      961 aaccttggga ttccacggcc actctccgtt tctgtcccgg aattccatga acgaacggaa
     1021 gagctcgatc cgttccagca ggctgcggag cgaaggctgc ggcttcagac gatccagcgc
     1081 gaacagtccc agttcgcccc ggacggacga cgcaaagacg ttgagtatct tgggtatctc
     1141 gagaattcgt gagacgtctt ctttaatttc catccgagga gcctccgtcc gtctcttctc
     1201 ccgcctgcgg gaagagggat gggaggttca gctcgggaag cgtaaagttc tccgggaaga
     1261 tgttccactc cctcatacgc gcctggacaa ccggccaggc ggcatgcgcg ccccgcatca
     1321 ccacgctttc gcccatccat tccgaaggga tgatcggcga ataggtcgtg atgccgacat
     1381 ataggaagag gatgagcgcg gcgcccttga gaagaccggc gccggcgccg aggattctgt
     1441 cgatgaacgc gagcgatgtg aacttcaaaa aagcgcgtac cattttacac atgagcgccg
     1501 caccgacgac cgctgcgata tagataagcg ccatg
//
LOCUS       DAOTIL010000751         1534 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminobacteriaceae bacterium isolate AMR_MDS_3026
            NODE_97332_length_1534_cov_2.830967, whole genome shotgun sequence.
ACCESSION   DAOTIL010000751 DAOTIL010000000
VERSION     DAOTIL010000751.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868050
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminobacteriaceae bacterium (wastewater metagenome)
  ORGANISM  Aminobacteriaceae bacterium
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae.
REFERENCE   1  (bases 1 to 1534)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1534)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:19:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,224
            CDSs (total)                      :: 3,198
            Genes (coding)                    :: 3,184
            CDSs (with protein)               :: 3,184
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1534
                     /organism="Aminobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_97332_length_1534_cov_2.830967"
                     /isolate="AMR_MDS_3026"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:3034609"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..406
                     /locus_tag="P5201_12870"
     CDS             <1..406
                     /locus_tag="P5201_12870"
                     /inference="COORDINATES: protein motif:HMM:NF015692.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ADP-ribosylglycohydrolase family protein"
                     /protein_id="HRV99482.1"
                     /translation="AQALAGSVFLARRAASNGTGASCKEEVRAFLERRCGYSLRRTVA
                     EIRPDYTFDVTCQGSVPESLICFLEASDFESAVRKAVSLGGDADTQACIAGAVAGALW
                     GVPEHIWKRVRSTLDASMLHILDAFEERFAKE"
     gene            complement(577..1014)
                     /locus_tag="P5201_12875"
     CDS             complement(577..1014)
                     /locus_tag="P5201_12875"
                     /inference="COORDINATES: protein motif:HMM:NF016246.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF488 domain-containing protein"
                     /protein_id="HRV99483.1"
                     /translation="MIKLFTIGCSGRSARDFFGALGKAQIQTVIDIRLRNTSHLAGFS
                     KMQDLPFFLESICGAAYIHIPQLAPTERILDDFKKNKGSWDEYETAYLRLLEERGAEH
                     LLSPAFLHMGCLLCSETKSDYCHRRLASDFFARKFSEVSITHL"
     gene            complement(1011..>1534)
                     /locus_tag="P5201_12880"
     CDS             complement(1011..>1534)
                     /locus_tag="P5201_12880"
                     /inference="COORDINATES: protein motif:HMM:NF016246.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF488 domain-containing protein"
                     /protein_id="HRV99484.1"
                     /translation="IEAVADIRSAPFSRRFPAFNKESLRSILAKRGIAYVFLGKELGA
                     RPADPSCYRDGRAEFDLIAGSEAFRNGIARLLKGASRMRIVLLCAEKEPLHCHRCILA
                     GRVLAEQGEDVVHILADGSAERHEETMQRLLVLYGKTEDDLFLSPQERVRLVCEMQEK
                     KMAHMLKNGDEDE"
BASE COUNT          331 a          453 c          429 g          321 t
ORIGIN      
        1 ggcgcaggcg cttgccggca gcgtctttct cgcccgtaga gccgcatcga acgggaccgg
       61 ggcgtcctgc aaggaggaag tccgcgcctt cctcgagcgc cgctgcggat acagtctgcg
      121 gaggaccgtc gccgagatcc gtcccgacta cacgttcgac gtcacctgcc aggggagcgt
      181 gccggaatct ctcatctgtt ttctcgaggc gtcggacttc gagagcgccg ttcgcaaagc
      241 cgtctcgctg ggcggggatg cggatacgca ggcatgcatc gccggcgctg tagcgggagc
      301 cctctggggt gtgccggaac atatatggaa gcgggtgcgg agcacgctcg acgcatccat
      361 gctccacatt ctggacgctt tcgaggagcg cttcgcgaag gaataacatg cgaccgtaca
      421 gttgaacacc cctgaagagg cctgaaaagg tctctttttt tgtgcgggaa atcctgtgtt
      481 caggtgaagt cccgcaactg cgcctctgaa agcggatatc cgtggctctc aaccggatac
      541 aggcggcttt ggtaatggag ataaatggct ctatcgttat aaatgcgtta tcgacacttc
      601 ggaaaatttc cgagcgaaaa aatcagaagc cagtctgcga tgacagtaat cggacttcgt
      661 ctcgctgcag agcaaacagc ccatgtgcag aaaagcggga gagagcaggt gttccgcacc
      721 ccgttcttca agcaggcgca aatatgccgt ctcgtattcg tcccagcttc ccttgttttt
      781 tttgaaatca tccaggatcc tctccgtagg ggccagctgg ggaatatgaa tatacgcggc
      841 cccgcagatg ctctcaagga agaacggcag atcctgcatc tttgaaaaac cggcgagatg
      901 cgatgtgttg cgaaggcgga tatcgatcac ggtttgtatt tgcgccttcc cgagcgcgcc
      961 gaaaaaatcc cgggcgcttc tgcccgaaca tcctatcgta aacagcttga tcattcgtct
     1021 tcatccccat tcttgagcat gtgcgccatc ttcttctcct gcatctcgca cacaagccgt
     1081 acacgctcct gcggagagag aaacagatcg tcttctgttt tgccataaag caccagcaac
     1141 cgctgcatgg tctcttcatg gcgctccgcc gaaccgtccg ccagaatatg aacaacatcc
     1201 tccccctgtt cggcaagcac tcgccccgcg agaatgcacc ggtggcagtg caagggctcc
     1261 ttctccgcgc acagaagaac tatacgcatg cgggaagctc ctttcagaag ccgtgctatc
     1321 ccgttccgga acgcttcgct tccagcgatc aggtcgaact cggcgcggcc gtctctgtag
     1381 caggacgggt cggcgggacg cgcccccagc tcctttccaa gaaaaacata ggcgatacct
     1441 ctcttcgcca gaatactgcg cagagattct ttgttgaagg ccgggaaacg gcggctgaaa
     1501 ggagccgagc gaatgtccgc gaccgcctcg atgc
//
LOCUS       DAOTIL010000752         1530 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminobacteriaceae bacterium isolate AMR_MDS_3026
            NODE_97665_length_1530_cov_3.448136, whole genome shotgun sequence.
ACCESSION   DAOTIL010000752 DAOTIL010000000
VERSION     DAOTIL010000752.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868050
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminobacteriaceae bacterium (wastewater metagenome)
  ORGANISM  Aminobacteriaceae bacterium
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae.
REFERENCE   1  (bases 1 to 1530)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1530)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:19:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,224
            CDSs (total)                      :: 3,198
            Genes (coding)                    :: 3,184
            CDSs (with protein)               :: 3,184
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1530
                     /organism="Aminobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_97665_length_1530_cov_3.448136"
                     /isolate="AMR_MDS_3026"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:3034609"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..500)
                     /locus_tag="P5201_12885"
     CDS             complement(<1..500)
                     /locus_tag="P5201_12885"
                     /inference="COORDINATES: protein motif:HMM:NF026042.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-binding protein"
                     /protein_id="HRV99485.1"
                     /translation="MDAIRRMIKDRCMEKEEPFCASACPFHLDVREFIARMQRGAFNT
                     AFRLFSNTTGFPAIVAAHCHEPCAAVCPRGTVDAPVQLNLLEKAAVAYAANTKPNSYN
                     LPPKKGRIAVVGAGLSGLGCALRLANRKYRVDVYERSGRVGGRLWDEMDPEIFLADIR
                     LQFSNE"
     gene            complement(572..>1530)
                     /locus_tag="P5201_12890"
     CDS             complement(572..>1530)
                     /locus_tag="P5201_12890"
                     /inference="COORDINATES: protein motif:HMM:NF014758.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HRV99486.1"
                     /translation="FTKYDTYRELGQGGDIGSLMLTLEALKPLGVTPEQIRQVQNDTK
                     LCPDAGMSGASRTHFMSGNATIIAAGKLIAAMKKPDGTYRTYEEMKAEGLETKYFGAY
                     SNTSVPGLCRLDPNTGIGDPTPAFTYCLNLAEVEVDTSTGKTSVLRFTCVDDVGKIGN
                     IDAVNGQAYGGISHAIGFALGENYDDVKKHTNIAASGVPYIETIPDEIEVIHYERPDE
                     AGPFGSSGASEGFQSSGHMAVLNAINNACGVRVYEMPATPEKVKAGLALLASGGTIEP
                     PKKYFLGSDLYEELENIKANPVQVGPEDYFVPLGGASAERFF"
BASE COUNT          259 a          472 c          479 g          320 t
ORIGIN      
        1 tcttcgttcg agaactggag gcgtatatcg gcgaggaaga tctccggatc catctcgtcc
       61 caaaggcgtc cgccgacgcg gccgcttcgc tcgtagacgt ccacacggta tttcctgttc
      121 gccagccgca gcgcgcaccc caggccgctc aaccccgctc cgaccacggc gatcctcccc
      181 ttcttcgggg ggaggttgta gctgttcggc ttggtattcg ccgcatacgc gacggccgct
      241 ttttcgagga gattgagctg tacgggagcg tcgaccgttc ctctcggaca gaccgcggcg
      301 cacggctcgt ggcagtgcgc cgcgacgatc gcgggaaagc cggttgtgtt cgagaagaga
      361 cggaatgcgg tgttgaaagc gccccgctgc atccgcgcga tgaattcgcg cacgtcaaga
      421 tggaacggac aggccgaggc gcagaaaggc tcctccttct ccatgcagcg atcctttatc
      481 atgcgtctga ttgcatccat cgtccaatcc cctccggaga tgccgggagc ccgtatcttc
      541 agcagataca ggctcccatg aacatgtttt cttagaagaa gcgctccgcg gaggcgccgc
      601 ccagaggaac gaagtagtcc tcgggcccga cctggaccgg gttggccttg atgttctcca
      661 gctcctcgta gagatccgag ccgaggaagt acttcttcgg cggctcgatg gtcccgcccg
      721 aagccagcag agcaagcccg gccttgactt tttcgggagt ggcgggcatt tcgtagacgc
      781 ggacgccgca cgcattattg atcgcgttga gcaccgccat gtgcccggag gactggaagc
      841 cctcggatgc gcccgaagaa ccgaagggac cggcttcgtc cggacgctcg tagtggatga
      901 cttcgatctc gtccggaatg gtctctatgt aggggacgcc gctcgcggca atgttggtgt
      961 gctttttaac gtcgtcgtag ttttcgccga gcgcgaagcc gattgcgtgc gagatgccgc
     1021 cgtacgcctg tccgttgacg gcgtcgatgt ttcctatttt ccccacgtcg tcgacgcacg
     1081 taaagcgcag cacgctcgtc ttcccggtgg aagtgtcgac ctcgacttcg gccaagttaa
     1141 ggcaataggt aaaagcgggc gtcggatcgc cgattcccgt gttcgggtcg agcctgcaca
     1201 gcccggggac cgaagtgttc gagtaggcgc cgaagtactt cgtctccaac ccttcggcct
     1261 tcatctcctc gtaggttctg taggtcccat cgggcttctt catggcggcg atgagcttcc
     1321 cggcggcgat gatggtggcg ttgccgctca tgaagtgcgt gcggctcgcc ccggacatcc
     1381 ccgcgtcggg gcagagcttc gtgtcgttct gcacctgccg gatctgctcg ggagtgacgc
     1441 cgagaggttt cagcgcctcc agcgtgagca tcagggagcc gatgtcgccg ccctgaccga
     1501 gctcccggta ggtgtcgtac ttcgtgaacg
//
LOCUS       DAOTIL010000753         1527 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminobacteriaceae bacterium isolate AMR_MDS_3026
            NODE_97966_length_1527_cov_2.783288, whole genome shotgun sequence.
ACCESSION   DAOTIL010000753 DAOTIL010000000
VERSION     DAOTIL010000753.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868050
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminobacteriaceae bacterium (wastewater metagenome)
  ORGANISM  Aminobacteriaceae bacterium
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae.
REFERENCE   1  (bases 1 to 1527)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1527)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:19:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,224
            CDSs (total)                      :: 3,198
            Genes (coding)                    :: 3,184
            CDSs (with protein)               :: 3,184
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1527
                     /organism="Aminobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_97966_length_1527_cov_2.783288"
                     /isolate="AMR_MDS_3026"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:3034609"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            573..>1527
                     /gene="ltrA"
                     /locus_tag="P5201_12895"
     CDS             573..>1527
                     /gene="ltrA"
                     /locus_tag="P5201_12895"
                     /EC_number="2.7.7.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016241516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003964 - RNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0000373 - Group II intron splicing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="group II intron reverse transcriptase/maturase"
                     /protein_id="HRV99487.1"
                     /translation="MLRIVSDPIWLEEAICKTLGARGATTPGIDGMTRAHVENNIVEL
                     ARTIGKELRAGTYEPQPVRRVNIRKANGKLRPLGIPTLKDRIVQRAMLMAMDPIWESD
                     FHNRSYGFRPCRSVHQAIRTVKVQLQDFGRGNGPRWIIEGDLTSYFDTIHHRLLMKAV
                     RRRIRDRRFLNLLWKFLKAGHVDKRLFHAAHDGVPQGGVISPLLGNIMLNEFDTYLEN
                     RYLSTKTRRARESWNDSVKRKRPKAIEEGREWKPSVSYCRYADDFLISVKGTRAQAEE
                     IREECREYLHEDLRLVLNMEKTRITHVNDGFVFLGHRIIRKR"
BASE COUNT          404 a          413 c          473 g          237 t
ORIGIN      
        1 tgatgtagcc cgccgacaaa ggggactgag gcgagaccgt tacgccgaga tggtcttcga
       61 gactgagcgt cgaaaggtcg aggaacacga accgattgat tcgcatccga gggtcgaatc
      121 cccaccgcat cctacacggt ctgacaggat gcatgcgggg ggaggtaccc ggcgacggga
      181 ggaagcctcc gaagccatct gcttattcct tgctggcaga tggtggagcg acggggagaa
      241 cgcagccaga ccgtcgactc cgcatcgact tgcagcacgc aagggcaagg attgcgatcg
      301 gcggcctcat cgacgcgctt gagttcaatc gcgtgaagcg gggaagcttc gcacggccga
      361 tgggaggcgg gagctccggg ccgcgcgggg tggcggagca cccgtagtag tccgagacgg
      421 ggaaagcccg tcacatggcg aaggggtgca gcttgacgga tttacctgat agactatctg
      481 acccagcgag gtgaagacct ctgataatca gagaaataca gcgtaaatta gcgacatggt
      541 ccgaagagaa caaggcgaga aggttctacc ggttgctgcg aatcgtctcc gacccgatat
      601 ggctggaaga ggcgatatgc aagaccctcg gagcccgggg agcgacaacg ccgggaatcg
      661 acgggatgac gagagcccac gtggagaaca acatcgtgga actcgcgcga accatcggga
      721 aagaactcag agcgggcacg tacgaaccac agcccgtgcg gcgcgtcaac atcaggaagg
      781 ccaacggcaa actcagaccg ctcgggatac cgacgctcaa ggacagaata gtgcagcgag
      841 caatgctcat ggcgatggac cccatatggg agagcgactt ccacaaccgg tcctacggat
      901 tcaggccctg cagaagcgtg catcaggcca tacgaaccgt gaaggttcaa cttcaggact
      961 tcggaagagg aaatggcccc cgatggataa tcgaagggga tctgacgagc tacttcgaca
     1021 ccatacatca cagactgctc atgaaagccg tgaggaggag aatccgggac aggcgctttt
     1081 tgaacctgct ttggaaattc ctcaaggcgg ggcacgttga caagcggctc tttcacgccg
     1141 cgcacgacgg cgtgcctcaa ggcggagtca tctctccgct gctcggaaac atcatgctga
     1201 acgaattcga cacgtacctt gaaaaccgat atctctcaac gaagacgcga agagccagag
     1261 aaagctggaa cgacagcgtc aagcggaagc gtcccaaagc aatcgaagaa ggacgcgagt
     1321 ggaagccttc cgtgagctac tgccgatacg ccgacgactt tctgatatcc gtcaaaggaa
     1381 cacgagcgca ggcggaggag atacgcgaag agtgcaggga atatctgcac gaagacctcc
     1441 gacttgtcct gaacatggaa aagacccgca taacccacgt caacgacgga ttcgtctttc
     1501 tcggacaccg catcatcaga aagcggg
//
LOCUS       DAOTIL010000754         1526 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminobacteriaceae bacterium isolate AMR_MDS_3026
            NODE_98056_length_1526_cov_2.844324, whole genome shotgun sequence.
ACCESSION   DAOTIL010000754 DAOTIL010000000
VERSION     DAOTIL010000754.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868050
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminobacteriaceae bacterium (wastewater metagenome)
  ORGANISM  Aminobacteriaceae bacterium
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae.
REFERENCE   1  (bases 1 to 1526)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1526)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:19:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,224
            CDSs (total)                      :: 3,198
            Genes (coding)                    :: 3,184
            CDSs (with protein)               :: 3,184
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1526
                     /organism="Aminobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_98056_length_1526_cov_2.844324"
                     /isolate="AMR_MDS_3026"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:3034609"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..500
                     /locus_tag="P5201_12900"
     CDS             <1..500
                     /locus_tag="P5201_12900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006299797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="TRAP transporter large permease subunit"
                     /protein_id="HRV99488.1"
                     /translation="FASLYEALKDAVLVNGATSFMVGFSMAFAAYLAMAQIPAKLGAW
                     LGGFSTDTIVIMLLLNFILLVIGCFVDNIAAVIILTPILLPVVTRLGVDPVQFGVIIT
                     VNLAIGFVTPPYGINLFVASAVSGESIEKISKNIMPFVLSMGFALLLITFIPSLSLGL
                     VRLLR"
     gene            516..>1526
                     /locus_tag="P5201_12905"
     CDS             516..>1526
                     /locus_tag="P5201_12905"
                     /inference="COORDINATES: protein motif:HMM:NF021387.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050043 - lactate racemase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lactate racemase domain-containing protein"
                     /protein_id="HRV99489.1"
                     /translation="MWPEHIPYVLETGAGPEFPRMFRVEQLFPRKRLDDVPAAATASM
                     EALALPDLSGKRIAVAAGSRGIADIVPALRSVVAFLKKRGAEPFIVPAMGSHGGATAE
                     GQLHILEGLGVTESSVGAPVRSSMEATLLGRTEDGIDVWCDTNAFESDGIVLCNRVKA
                     HTDFKMSVESGLCKMMAVGLGKHKGATAIHGRGVARLGPSILSAATLFLERAPVLFGL
                     AIVENAYEETMIVEAIPPERCIQREEELLREAKGAMGRLIPKDIDILVVEQIGKEISG
                     AGMDPNISGRPMKGALGFEEIASPEVVVVLGLTEHSAGNAIGIGLADITKLRAVRGLD
                     FAS"
BASE COUNT          255 a          497 c          502 g          272 t
ORIGIN      
        1 ccttcgcgtc gctttacgag gcgctgaagg acgcggtact cgtcaacggg gcgacctcct
       61 tcatggtggg cttctccatg gcgttcgccg cctacctggc catggcgcag atcccggcca
      121 agctgggggc gtggctcgga ggattctcca cggacaccat cgtcatcatg ctgctgctga
      181 acttcatcct cctcgtgatc ggctgcttcg tggacaacat cgccgccgtc atcatcctga
      241 cgcccatcct tctgccggtg gtcacgcgcc tcggcgtcga ccccgtccag tttggcgtca
      301 ttatcaccgt gaacctcgcg atcggcttcg tcacgccccc ctacgggatc aatctcttcg
      361 tggcgtcggc cgtctcggga gagagcatag aaaagatctc caaaaacatc atgccgttcg
      421 tcctctcgat gggattcgcc ctgctgctga tcacgttcat accgtcgctg agcctcggcc
      481 tggtgcggtt gcttcggtag aaaggagaga acgtcatgtg gcctgaacat attccctacg
      541 tgctcgaaac gggagcgggg ccggaatttc cccgcatgtt ccgcgtggaa cagctctttc
      601 cccgaaaacg cctcgacgac gtccccgccg ccgcgaccgc ctcgatggag gcgctggcgc
      661 ttccggacct ctccggaaag cggatcgccg ttgcggcggg gagccggggg atagcggaca
      721 tcgtccccgc gctgcgctcc gtcgtcgcct tcttgaagaa gcgaggcgcc gaaccgttca
      781 tcgtcccggc gatggggagc cacggcggcg cgaccgccga gggtcagctg catatcctgg
      841 aggggctggg cgtcacggag tcgtccgtcg gcgcgcccgt tcgctcgtcc atggaagcga
      901 cgctcctcgg caggacggag gacggaatcg acgtctggtg cgacacgaac gccttcgagt
      961 cggacggcat cgtcctctgc aaccgggtga aggcgcacac ggacttcaag atgtccgtgg
     1021 agagcggcct gtgcaagatg atggcggtgg gtttgggcaa gcacaagggg gcgaccgcca
     1081 tccacggacg gggagtcgcc aggctggggc cgagcatcct ctccgcggcg acgctcttcc
     1141 tggagcgcgc ccccgtcctc ttcggcctcg ccatcgtcga gaacgcctac gaggagacga
     1201 tgatcgtcga ggcgataccg cccgaacgct gcatccaacg cgaggaggag ctgctccgcg
     1261 aggcgaaggg cgcgatgggg cggctcattc cgaaagatat cgacatcctg gtggtggaac
     1321 agatcggcaa ggagatcagc ggggcgggga tggacccgaa catctcgggg cgtccgatga
     1381 agggggcgct cggcttcgag gagatcgcct cccccgaggt cgtcgtcgtg ctcggactga
     1441 cggagcactc cgccggcaac gccatcggca tcggtctcgc cgacatcacg aagctgcgcg
     1501 ccgtccgggg gctggacttc gccagc
//
LOCUS       DAOTIL010000755         1526 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminobacteriaceae bacterium isolate AMR_MDS_3026
            NODE_98058_length_1526_cov_2.825289, whole genome shotgun sequence.
ACCESSION   DAOTIL010000755 DAOTIL010000000
VERSION     DAOTIL010000755.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868050
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminobacteriaceae bacterium (wastewater metagenome)
  ORGANISM  Aminobacteriaceae bacterium
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae.
REFERENCE   1  (bases 1 to 1526)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1526)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:19:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,224
            CDSs (total)                      :: 3,198
            Genes (coding)                    :: 3,184
            CDSs (with protein)               :: 3,184
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1526
                     /organism="Aminobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_98058_length_1526_cov_2.825289"
                     /isolate="AMR_MDS_3026"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:3034609"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(160..>1526)
                     /locus_tag="P5201_12910"
     CDS             complement(160..>1526)
                     /locus_tag="P5201_12910"
                     /inference="COORDINATES: protein motif:HMM:NF033559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="IS1634 family transposase"
                     /protein_id="HRV99490.1"
                     /translation="DDAVGRLLDNLSEYNISVLYERFVLECLAPLLHGTVNLHVDTTN
                     FSVHGEYVPNEGFKWEIAFGHAKDGRTDLKRFVLNMVADGSGIPLFLELLSGNASDKK
                     SIVVGMEKATNTLAALGESPLFGIADAAFYSAENIARIHGSWISRVPATLNEAAALLE
                     TGEALLPCSDPRYSFLEKTSSYGGVEQRWFLFRSDEMAGKQEKTLEKRLERKLTDAKK
                     DASALSKTEFFCEADARSAADSFFSEHPLVQGSVLVEELRRKKGGRGRPASGAEMEIR
                     YRAKIDPALAPDAVEILRSKCGRFILATSAVGETAPGAEEILGLYKEQSAVEKGFRFL
                     KDKSFHAAEIYLKREERIEGLMFLMTIALAVYNLAEKELREVLSASGESIPAPGGKPM
                     KNPTLDRLFHLFARVTVLVGEECGKRTQRVMNLNDTLKRMLALFGKNFERYYMDGASS
                     NAYG"
BASE COUNT          279 a          444 c          472 g          331 t
ORIGIN      
        1 agccgggtgt cgtatgagtg agaaagagcg aaccttcctg tcaagaagcg caagatagaa
       61 cgattgggga agttggcttc cccaatcgtt ccggcgcata tccacgctgc cggaagcgtc
      121 aatccgacat tccgcactga catttagaga tttttgtatt tatccatatg cgttcgagga
      181 ggcgccgtcc atgtagtacc gttcgaagtt tttcccaaat aaagcgagca tccgcttgag
      241 cgtgtcgttc aggttcatca cgcgctgggt ccgctttccg cactcctcgc cgaccagtac
      301 cgtgacccgc gcgaaaaggt gaaagagacg gtccagggtg ggattcttca tcggcttccc
      361 tcccggagcg ggaatgctct ctccgctcgc ggacagcact tcccggagtt ctttctcggc
      421 aagattgtag accgccagag cgatcgtcat gaggaacatg aggccttcga tgcgctcctc
      481 ccgcttcaga taaatctcgg cggcgtggaa ggatttgtcc ttgaggaagc ggaagccctt
      541 ctcgacggcg ctctgctcct tgtacagccc gagtatctcc tcggcgccgg gggctgtttc
      601 tcctaccgcg ctggtggcga gtatgaagcg accgcacttg cttcgaagga tctcgacggc
      661 gtcgggagcg agcgcgggat cgatcttcgc tcggtaccgg atctccatct ccgctcccga
      721 cgcgggacgt cctcttccgc ctttcttgcg gcggagctcc tcgacgagta cgcttccttg
      781 aaccagcgga tgctcggaga agaaggaatc ggccgcggag cgggcgtcgg cctcgcagaa
      841 gaattccgtt ttcgacagcg cggatgcgtc cttcttcgcg tcggtcagct tccgctcgag
      901 gcgtttttcg agcgtcttct cctgcttccc ggccatctcg tcggagcgga agaggaacca
      961 gcgctgctcg acgcccccgt aggaagaggt cttctcaagg aaggagtagc gggggtcgga
     1021 gcagggaaga agggcttccc cggtttcgag aagcgccgcg gcctcgttca gcgtcgcggg
     1081 tacccggctg atccacgagc cgtgtatgcg ggcgatgttc tccgcgctgt agaaagcggc
     1141 gtcggctatg ccgaacagcg gagattcgcc gagtgcggcg agcgtgttcg tcgccttctc
     1201 catgccgacc acgatgctct ttttgtccga ggcgttgccg gagagcagtt cgagaaagag
     1261 cggaatcccg cttccgtcgg cgaccatgtt cagcacgaag cgcttcaggt cggttctgcc
     1321 gtccttggcg tggccgaacg cgatctccca tttgaacccc tcgttcggaa cgtactcgcc
     1381 gtgcacgctg aaattcgtcg tgtcgacgtg aagattgacg gttccatgga gcaagggggc
     1441 gagacattcg aggacgaaac gctcgtagag aacggagatg ttgtattcgc tcaggttgtc
     1501 gagaagacgt cccacggcgt cgtcgt
//
LOCUS       DAOTIL010000756         1526 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminobacteriaceae bacterium isolate AMR_MDS_3026
            NODE_98062_length_1526_cov_2.789939, whole genome shotgun sequence.
ACCESSION   DAOTIL010000756 DAOTIL010000000
VERSION     DAOTIL010000756.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868050
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminobacteriaceae bacterium (wastewater metagenome)
  ORGANISM  Aminobacteriaceae bacterium
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae.
REFERENCE   1  (bases 1 to 1526)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1526)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:19:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,224
            CDSs (total)                      :: 3,198
            Genes (coding)                    :: 3,184
            CDSs (with protein)               :: 3,184
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1526
                     /organism="Aminobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_98062_length_1526_cov_2.789939"
                     /isolate="AMR_MDS_3026"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:3034609"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..574)
                     /locus_tag="P5201_12915"
     CDS             complement(<1..574)
                     /locus_tag="P5201_12915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008710839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HRV99491.1"
                     /translation="MTQPTAPHATSKVSLLKRDPALLPALIAIWISLAVFVLYPLFRL
                     FLTTFVVEGQLSFTNLAVVLESWYDRQAFVNSIWLATAVSVAGTFLGYVYAFAVTRIP
                     MPLWLKWFLGAVTTLPLISPPFTSSIALTLSLGPNGILLKLLGIGNFNFYGFWGTWMS
                     ETLTYFPVSFLTLAAVLSAIDPNLEDAGLSL"
     gene            complement(669..>1526)
                     /locus_tag="P5201_12920"
     CDS             complement(669..>1526)
                     /locus_tag="P5201_12920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009163986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HRV99492.1"
                     /translation="ETGVKVNFMRFPSGEALARVVAEKNNPQVDMILGGPADTYEAGI
                     KEGVFEQYKPAEADMIPERFRSPENYWTGVGIIPLVFLTNGKFLKEKGLEAPASWNAL
                     LDPAYKNGLQMADARTSGTATERIYALVKIMGEDEAFAYQKKLHGNIQMYTKSGAGGA
                     MPIATGQAASGIFYIVDALEIQQQGHDVVLSYPVEGVSFGIEATGILKGAKNLENAKK
                     FMDWASSKTFAQLLVDRKINYIPTRTDVETTNPALDVSKVKLVEVDVAWKGANRKAFV
                     DRWINEVIK"
BASE COUNT          293 a          446 c          493 g          294 t
ORIGIN      
        1 ccagcgagag cccggcgtcc tcgaggttcg ggtcgatagc ggagagcacc gccgccaggg
       61 tgagaaaaga aacggggaaa taggttaaag tctccgacat ccacgtcccc cagaagccgt
      121 agaagttgaa gtttccgatg cccagcagct tcagcagtat gccgttgggc cccagcgaga
      181 gggtaagcgc gatgctgctc gtgaaggggg gcgagatcag ggggagcgtg gtgacggccc
      241 ccaggaacca cttcagccag agaggcatcg gaatccgcgt caccgcgaag gcgtagacgt
      301 atccgaggaa ggtccccgcg acggagacgg ccgtggccag ccagatgctg ttgacgaagg
      361 cctgccggtc gtaccagctt tcgagcacaa ctgcgaggtt cgtgaacgag agctgcccct
      421 ccacgacgaa cgtggtcaga aaaagccgga agagcgggta gagaacgaag accgcgagcg
      481 atatccagat ggcgatgagc gcggggagca gcgcgggatc ccgcttcagg agagatactt
      541 tactcgttgc gtgcggcgcc gttggttgtg tcataaattc aatctccttg cctgaacatc
      601 aaaggtaaaa aacccggcgg aaagagcgtc cgccgggggc aggttttcca aaaaccggaa
      661 tgagtccgct acttgatgac ctcgttgatc cagcggtcga caaaggcctt ccggttggcg
      721 cccttccacg caacgtcgac ctcgaccagc ttgaccttag aaacgtcgag cgcggggttc
      781 gtggtctcca cgtcggtgcg cgtcgggatg tagttgatct tcctgtcgac gagcagctgc
      841 gcgaacgtct tcgacgacgc ccagtccatg aacttcttcg cgttttcaag gtttttcgcg
      901 cccttcagaa tgcctgtggc ctcgatgccg aaggagactc cctcgacggg gtagctcagc
      961 accacgtcgt gcccctgctg ctggatctcc agcgcgtcca cgatgtagaa gattccggag
     1021 gcggcctgtc cggtcgcgat gggcatcgcg ccgcccgcgc cgctcttggt gtacatctgg
     1081 atgttcccgt ggagcttctt ctgataggcg aacgcctcgt cctcgcccat gatcttcacg
     1141 agggcgtaga tgcgctcggt cgccgtgccg gaggtgcggg cgtcggccat ctggaggccg
     1201 ttcttgtacg ccgggtcaag aagagcgttc caggaggcgg gtgcttcaag ccccttctcc
     1261 ttgaggaact ttccgttggt caggaagacc agagggatga tgcccacgcc ggtccagtag
     1321 ttctcgggag agcggaagcg ctccgggatc atgtcggcct cggcgggttt gtactgttcg
     1381 aagacgccct ccttgatgcc ggcttcgtag gtgtcggcgg ggcctccgag gatcatgtcg
     1441 acctgcggat tgttcttctc cgccaccact ctggccagcg cctctccgga cgggaagcgc
     1501 atgaagttca ccttgacgcc ggtctc
//
LOCUS       DAOTIL010000757         1521 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminobacteriaceae bacterium isolate AMR_MDS_3026
            NODE_98528_length_1521_cov_2.966576, whole genome shotgun sequence.
ACCESSION   DAOTIL010000757 DAOTIL010000000
VERSION     DAOTIL010000757.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868050
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminobacteriaceae bacterium (wastewater metagenome)
  ORGANISM  Aminobacteriaceae bacterium
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae.
REFERENCE   1  (bases 1 to 1521)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1521)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:19:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,224
            CDSs (total)                      :: 3,198
            Genes (coding)                    :: 3,184
            CDSs (with protein)               :: 3,184
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1521
                     /organism="Aminobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_98528_length_1521_cov_2.966576"
                     /isolate="AMR_MDS_3026"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:3034609"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(80..1072)
                     /gene="hypE"
                     /locus_tag="P5201_12925"
     CDS             complement(80..1072)
                     /gene="hypE"
                     /locus_tag="P5201_12925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013048783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase expression/formation protein HypE"
                     /protein_id="HRV99493.1"
                     /translation="MNTVTLGHGSGGRQSHELVEMLLEGFRKRSIGPELEDAALLPGG
                     YAMTVDGFTVSPRKFPGGDIGHLCVCGCANDLAVRGASPDFLGIGLIIEEGFPSGELA
                     EYMESAAATCESLGMTLAAGDTKVVPSGAVDGVYFSGSAFGRVHTVHPLSMSNIREGD
                     SLIVSTSIGRHGAAIAAARFNLSVDGLESDCAALWPLIKPLLSFEGLRCMRDCTRGGL
                     GTVLCEWAESTNLHISLDEASLPVDDRVRSLCDVLGFDPLYMACEGCIVLAVSADSAR
                     GILDTLRNSPFCADAAIIGTVTRGEPRGMVSMRTSIGGERLVDMPFGELLPRIC"
     gene            complement(1069..1476)
                     /locus_tag="P5201_12930"
     CDS             complement(1069..1476)
                     /locus_tag="P5201_12930"
                     /inference="COORDINATES: protein motif:HMM:NF014034.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRV99494.1"
                     /translation="MALQKKQGEATLRSVYGQAVRPEGNTKARELMESVFISKDASWR
                     GLGILPDSGLVLREEFTRFDAEKRFGLSRLPGESPAGCRCGEVLTGRITPTECPLFRT
                     ACTPVSPVGPCMVSGEGTCGAYFRYHRTNGGKQ"
BASE COUNT          316 a          463 c          444 g          298 t
ORIGIN      
        1 gacaaagacg aaagagggag gcgcatatgc gcctccctct ttcgtctttg tccttgaagc
       61 ttccgggccg gaaatccctt cagcaaattc tgggaagcag ctccccgaac ggcatgtcaa
      121 caaggcgctc ccctccgata gaggtcctca tggaaaccat gcccctcggt tctcctcggg
      181 tgacggtccc gataatcgcg gcgtcggcgc aaaacggtga gttccgaagg gtgtcgagaa
      241 tccctcgggc ggagtctgcg gagacggcga ggacgatgca cccctcgcac gccatgtata
      301 acgggtcgaa tcctaaaacg tcgcataaag agcgtaccct gtcgtcaaca ggaagagacg
      361 cttcgtccag cgagatatgc aggttggtcg attccgccca ttcgcagaga acggttccca
      421 gtcctccgcg ggtgcagtct ctcatgcagc gaaggccttc gaatgacaag agcggtttga
      481 tgagcggcca gagcgctgcg cagtcgcttt cgagtccgtc gacggagagg ttgaacctgg
      541 ccgcggcgat cgcggcgccg tggcggccga tggaggtcga aacgatcagc gagtccccct
      601 cccggatatt cgacatgctg agaggatgga ccgtgtgtac tctcccgaaa gcgctcccgg
      661 agaagtagac gccgtctacg gctccggaag gaacgacttt ggtatcgcct gcggcaaggg
      721 tcattccgag ggattcgcag gtagcggccg cgctctccat gtattccgca agctccccgg
      781 aaggaaaccc ctcctcgata atcagtccta ttccgaggaa gtccggagaa gcgccccgga
      841 ctgcaaggtc gttcgcacag ccgcagacgc agagatgccc gatatctccg ccgggaaact
      901 ttcgggggga gactgtgaac ccgtcgaccg tcatagcgta gcctcccgga agaagggctg
      961 cgtcctccaa ttccggcccg atactccgtt tccggaaacc ttcaagcagc atttccacaa
     1021 gttcgtgact ctgtctgcct ccgcttccgt gcccaagcgt caccgtattc attgtttccc
     1081 tccgtttgtt ctgtgataac gaaagtacgc gccgcatgtg ccctctccgg aaaccatgca
     1141 ggggcctacc ggggagacgg gggtgcaggc tgtcctgaag agggggcact cggtaggagt
     1201 tatccttccg gtgagcactt cgccgcatcg gcagccggcc ggagactcgc cagggagacg
     1261 cgagagacca aatcgttttt cggcgtcaaa gcgagtgaat tcttcccgga gaacaagtcc
     1321 ggaatcggga agaattccaa gtcctctcca cgaagcgtct ttgctgataa aaacactctc
     1381 catgagctcc cgcgcttttg tgttcccttc ggggcgtacc gcctgtccgt agaccgagcg
     1441 aagagtcgcc tcaccctgtt ttttctgaag cgcgatcgct acgagcccgg cgccagccga
     1501 ccagccatat cagtccgatt a
//
LOCUS       DAOTIL010000758         1514 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminobacteriaceae bacterium isolate AMR_MDS_3026
            NODE_99169_length_1514_cov_3.804661, whole genome shotgun sequence.
ACCESSION   DAOTIL010000758 DAOTIL010000000
VERSION     DAOTIL010000758.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868050
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminobacteriaceae bacterium (wastewater metagenome)
  ORGANISM  Aminobacteriaceae bacterium
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae.
REFERENCE   1  (bases 1 to 1514)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1514)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:19:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,224
            CDSs (total)                      :: 3,198
            Genes (coding)                    :: 3,184
            CDSs (with protein)               :: 3,184
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1514
                     /organism="Aminobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_99169_length_1514_cov_3.804661"
                     /isolate="AMR_MDS_3026"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:3034609"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            34..771
                     /locus_tag="P5201_12935"
     CDS             34..771
                     /locus_tag="P5201_12935"
                     /inference="COORDINATES: protein motif:HMM:NF020052.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0006304 - DNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I restriction-modification enzyme R subunit
                     C-terminal domain-containing protein"
                     /protein_id="HRV99495.1"
                     /translation="MKAHHHFADPEWDGEPMEAEIPTRVGSPKPPKSDGKGSTDERGT
                     AARQKIRIRLADGKERTIQHMTCTSFWHPDGTPMSAQQFMELLFGRLPEFFKDEEELR
                     VLWSSPDTRRRLLEGLAEKGFGRDQLVEMQRIIDAEKSDLFDVLAHVAYARPPLLRET
                     RAQLARRGVWDEKRISEKQRAFLDFVLQHYVAVGVEELDREKLSPLLRLKYNDSMADA
                     LADLGRPAEIGAVFAGFQRYLYQEVSA"
     gene            844..>1514
                     /locus_tag="P5201_12940"
     CDS             844..>1514
                     /locus_tag="P5201_12940"
                     /inference="COORDINATES: protein motif:HMM:NF024700.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HRV99496.1"
                     /translation="MLISRLRLENWRNFRELDVPLRDVTYILGANASGKSNLLDVFRF
                     LRDVGKSVGGGLSAAVNSRGGFDDLRSLHSPSASEVLVDVELAEDADEQPLWRYVLGF
                     RSLENGMLAVSREEVARKGEILLRRPGSDDIQDDALLSQTHLEQTQANARFREIAAFF
                     SGIRYLHLVPQLLKYPERAGVRSLDDDPFGAGFLEKLAGTDDSVRRARLEMIEAFLRT
                     AVPGF"
BASE COUNT          314 a          398 c          476 g          326 t
ORIGIN      
        1 gatggttagg actacttcac gatctatgac ttcgtgaagg cgcaccacca tttcgccgat
       61 ccggagtggg acggcgaacc tatggaggcg gagattccga cgcgcgtcgg cagtccgaaa
      121 cctccgaaga gcgacgggaa aggatcgacc gacgagaggg gaaccgcggc gagacaaaag
      181 atacgtatcc ggctcgccga cggaaaggag cggacaatcc agcacatgac gtgcacgagt
      241 ttctggcatc ccgacggaac cccgatgtcc gcgcagcagt tcatggaact gctcttcgga
      301 agattgccgg agttcttcaa ggacgaggaa gagcttcgcg ttctctggag ttctccggat
      361 acgagacggc gcctgttgga agggctggct gaaaagggtt tcggaaggga ccagctcgtc
      421 gagatgcaaa gaatcatcga tgctgagaag agcgatctct tcgatgtcct tgctcatgtc
      481 gcctacgcca gaccgccgct tctccgcgag acgagagcgc agctggccag gcgcggcgtt
      541 tgggacgaga aacgaatcag cgagaagcaa agggcgtttt tggacttcgt tctacagcac
      601 tatgtcgccg tcggcgtgga ggaacttgac cgggagaagc tttcacccct gcttcgtctc
      661 aagtacaatg actctatggc ggacgcgctt gccgatctcg gaagaccggc ggagatcggc
      721 gccgtcttcg cagggtttca gcgctatttg tatcaggagg tctcggcgta ggcgagttgg
      781 ataatgagcg tatcggttga gtcgttttct tgctctgaag cgtcatcata aggagagata
      841 ttcatgctga taagccgact tcggctggaa aactggcgga acttcaggga actcgacgtt
      901 ccgcttcgcg atgtgacgta cattctgggg gcgaacgctt ccggaaaatc caatctgctg
      961 gatgtcttcc gttttctgcg cgatgtagga aaaagcgtgg gaggcggttt gtccgccgcc
     1021 gtgaacagtc ggggagggtt cgatgacctg cgttctctcc actcgccgtc agcttccgaa
     1081 gtcctggtgg atgtcgaact ggccgaggat gcggacgaac agcctttgtg gaggtatgtc
     1141 ctgggtttcc gttcgttgga gaacgggatg cttgctgttt cccgcgaaga ggtggcgcga
     1201 aagggcgaga tactgctgcg ccgtcccggt tccgacgaca ttcaggacga tgcgctgctc
     1261 tctcagacgc atctggagca gacgcaggcc aatgcgcgtt tccgtgagat tgccgcattc
     1321 ttttccggca ttcgctatct tcaccttgtg ccgcaactgc tgaagtaccc ggaaagggca
     1381 ggggtacgct ctctggacga cgatcctttc ggcgcaggat tcctggaaaa acttgccggc
     1441 acggatgatt ctgttcgccg cgcccgcctt gagatgatcg aagcattcct ccgaacggcc
     1501 gttcccggat tcga
//
LOCUS       DAOTIL010000759         1508 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminobacteriaceae bacterium isolate AMR_MDS_3026
            NODE_99729_length_1508_cov_3.442533, whole genome shotgun sequence.
ACCESSION   DAOTIL010000759 DAOTIL010000000
VERSION     DAOTIL010000759.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868050
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminobacteriaceae bacterium (wastewater metagenome)
  ORGANISM  Aminobacteriaceae bacterium
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae.
REFERENCE   1  (bases 1 to 1508)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1508)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:19:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,224
            CDSs (total)                      :: 3,198
            Genes (coding)                    :: 3,184
            CDSs (with protein)               :: 3,184
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1508
                     /organism="Aminobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_99729_length_1508_cov_3.442533"
                     /isolate="AMR_MDS_3026"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:3034609"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..469
                     /locus_tag="P5201_12945"
     CDS             <1..469
                     /locus_tag="P5201_12945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013048418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="adenosylhomocysteinase"
                     /protein_id="HRV99497.1"
                     /translation="ASRGDIFLTLTGNTKVIRKEHFEKMKDGVLLGNAGHFDVEISKP
                     DLDSLTESVEETRPGIRTYVLKDGRRLHLLGEGRLVNLACADGHPIEIMDLSFALQLE
                     SAIYMAKNSMKPGMYDVPAEIDRSVMEAKLASMNITLDTMTPEQEAYMKSWVE"
     gene            471..>1508
                     /locus_tag="P5201_12950"
     CDS             471..>1508
                     /locus_tag="P5201_12950"
                     /inference="COORDINATES: protein motif:HMM:NF014081.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HRV99498.1"
                     /translation="MATLFKNFLAVDGEMAEGRYCSLRVEDGRITAVADKLAALPGDE
                     VVDGERRMAIIPGFVNAHTHAAMSLLRGIGEEAPLMEWLQKKIWPAEANLTREHIYWG
                     TMAALLEMAACGTTAFADMYFEMDAVVEASLEAGMRCAACRGIVGGERGKFEEGLRLA
                     ETWKAHRDMITVQLGPHAPYTVPLQLMQEICAAAAERAMGVHFHFLETEWELSYLRDE
                     LKMTPKEYLESSGILAAPYGILAHCVWLDAETVGAVDFSRMAIAHNPNSNLKLGSGVM
                     DLTGLGGKTKKIALGTDGAASNNRLDIWGEMRCAALLHEGIRRDPMALSACDALRMAT
                     FEGASALGFKKK"
BASE COUNT          294 a          408 c          549 g          257 t
ORIGIN      
        1 cgcttcgcgg ggagatatct tcctgacgct caccgggaac acgaaggtga tccgcaagga
       61 gcacttcgag aagatgaagg acggcgtgct gctggggaac gcggggcact tcgacgtgga
      121 aatcagcaaa ccggatttgg acagcctgac ggagtccgtc gaggagacgc gccccggcat
      181 acggacgtat gtgctgaagg acggccggag actgcacctt ctcggcgaag gacgtctggt
      241 gaaccttgcg tgcgccgacg ggcaccctat cgaaattatg gatctgagct tcgcgctgca
      301 gcttgaatcc gccatctaca tggcgaaaaa cagcatgaaa cctgggatgt acgacgttcc
      361 tgcggagatc gaccggtcgg tgatggaggc gaagctggcc tcgatgaaca tcacgctcga
      421 cacgatgact cccgagcagg aagcgtacat gaagagctgg gtggagtagc atggcgaccc
      481 tcttcaagaa cttcctggcc gtcgacggcg agatggcgga ggggcgctac tgctctctcc
      541 gcgtcgagga cggcagaata acggccgtcg cggacaagct ggcggcgctg cccggcgacg
      601 aggtggtcga cggcgagcgg cggatggcga tcattcccgg gttcgtgaac gcgcacactc
      661 atgccgcgat gtcgctgctg cgggggatcg gcgaggaggc ccccctgatg gagtggctcc
      721 agaagaagat ctggcctgcc gaggccaatc tgacgcggga gcacatctat tggggaacta
      781 tggcggcgct gcttgaaatg gcggcctgcg ggaccactgc gtttgcggat atgtacttcg
      841 agatggacgc ggtggtggag gcgtcgcttg aggcgggaat gcgctgcgcg gcttgcaggg
      901 gtatcgtggg cggcgagcgg gggaagtttg aagaggggct gcgtcttgcg gagacgtgga
      961 aagcgcatcg cgacatgatc acggtgcagc tggggccgca cgcgccgtac accgtgccgc
     1021 tgcagctgat gcaggagatc tgcgcggcgg cggcggagcg cgcgatgggg gtgcacttcc
     1081 actttttgga gacggagtgg gagctctcgt acctccggga cgagctgaag atgacgccga
     1141 aggagtacct ggagtcctcc gggattctgg cggctccgta cgggattctg gcgcactgcg
     1201 tctggctgga tgcggagacc gtgggcgccg ttgatttttc cagaatggcg atcgcccata
     1261 accccaacag caacttgaag ctggggagcg gcgtgatgga tctcaccggc ctcggcggga
     1321 agacgaagaa gattgcttta ggaacggacg gggcggcgag caacaaccgc ctggacatct
     1381 ggggcgagat gcgctgcgcc gcgctgctgc acgagggaat ccgccgggat cccatggcgc
     1441 tttccgcgtg cgacgcgcta cggatggcca cgttcgaagg cgcttccgcc ctcggcttca
     1501 agaagaag
//
LOCUS       DAOTIL010000760         1508 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminobacteriaceae bacterium isolate AMR_MDS_3026
            NODE_99750_length_1508_cov_2.798348, whole genome shotgun sequence.
ACCESSION   DAOTIL010000760 DAOTIL010000000
VERSION     DAOTIL010000760.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868050
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminobacteriaceae bacterium (wastewater metagenome)
  ORGANISM  Aminobacteriaceae bacterium
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae.
REFERENCE   1  (bases 1 to 1508)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1508)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:19:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,224
            CDSs (total)                      :: 3,198
            Genes (coding)                    :: 3,184
            CDSs (with protein)               :: 3,184
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1508
                     /organism="Aminobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_99750_length_1508_cov_2.798348"
                     /isolate="AMR_MDS_3026"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:3034609"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..426)
                     /locus_tag="P5201_12955"
     CDS             complement(<1..426)
                     /locus_tag="P5201_12955"
                     /inference="COORDINATES: protein motif:HMM:NF016200.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter small permease subunit"
                     /protein_id="HRV99499.1"
                     /translation="MTSLKRFLKKIDALSELSGSVGKWCALLLVFAGTYEAVSRHFFN
                     APTVWAYDTLCMAGGALYLLGASYDYLHEAHTRVDIFYNMLPERGKAFMNVVCALFLF
                     FPLMGVMLWLSVTWAVRAWTINEVFFNSFWYPPAGPYRTI"
     gene            complement(461..>1508)
                     /gene="dctP"
                     /locus_tag="P5201_12960"
     CDS             complement(461..>1508)
                     /gene="dctP"
                     /locus_tag="P5201_12960"
                     /inference="COORDINATES: protein motif:HMM:NF015445.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="TRAP transporter substrate-binding protein DctP"
                     /protein_id="HRV99500.1"
                     /translation="ALRKSCWVLLLAVFVLGAFAPVSVEGAEAPKKIVWRSSGHGPAS
                     DPSQIYHDMLCKAITEASGGRLEIKPFVGGSIVPAYKEVDAVHENVLQLCYTCPMYNL
                     DKWSAAGLISSRPGALAGEALRTWFNYGGGADLMNKMMEGYNVMTFPGALSPLPEEVF
                     FHSKKKIESLEDLKAIKARCMGDGGEILKRMGMATVIIPGGELYEAMKRGTIDAFEYS
                     TLASNWNMHFNEVADYVYLSPVRAPSDPQVFFVNKDAWNALPKDLQLLVQTFVDRFSQ
                     AQHEFLVYESIIALEKFKEAGNQVLKVPEEVNVALAAEAAKFYEEKIASEPPIFGEIY
                     NSMKKFGEAYQTMK"
BASE COUNT          337 a          414 c          444 g          313 t
ORIGIN      
        1 aatggtacgg tacggccccg ccggggggta ccagaagctg ttgaagaaga cttcgttgat
       61 cgtccaggcg cgcactgccc acgtaacgga gagccagagc atgaccccca tcagagggaa
      121 gaaaaggaag agcgcacaga cgacgttcat gaacgccttc ccccgttcgg ggagcatgtt
      181 gtaaaaaatg tccacacgcg tgtgcgcctc gtggaggtag tcataggaag cgcccagaag
      241 atagagggcg cctcctgcca tacagagcgt atcgtacgcc cagaccgtgg gggcgttgaa
      301 gaagtgtctt gagacagctt catacgtccc ggcgaacaca aggagaaggg cgcaccattt
      361 ccccacggat ccggacagtt cgctaagtgc atcgattttt ttcaaaaacc gtttcaaaga
      421 ggtcataccg cttctccttg tgcgcgtcgt cgaagacgcg ttatttcatt gtctggtagg
      481 cttcgccgaa cttcttcatg gagttgtaga tctcgccgaa aatcggaggt tcgctggcta
      541 tcttctcttc gtagaacttc gcggcctcgg ctgcaagcgc cacatttact tcttcaggaa
      601 ccttcagcac ctggttgccg gcttccttga acttttcaag ggcgatgatg gactcgtaga
      661 cgaggaattc gtgctgcgcc tgagagaagc gatcgacaaa cgtttggacg aggagctgca
      721 gatccttcgg aagcgcgttc caggcatcct tgttcacgaa gaagacctgg ggatcgctgg
      781 gagcacgtac cggggagagg tagacgtagt cggcaacttc gttgaagtgc atgttccagt
      841 tggaggcaag cgtggagtac tcgaaagcgt cgatggtacc gcgcttcatt gcttcgtaca
      901 gctcgccgcc ggggatgatg accgtcgcca tgcccattct cttcaggatc tcgccgccgt
      961 cgcccatgca acgggccttg atcgccttca gatcctcaag actctctatt ttcttcttgg
     1021 agtggaagaa aacctcttca ggaagaggag agagcgcgcc ggggaaggtc atgacgttgt
     1081 acccttccat cattttgttc atcagatcag cgccgccgcc gtagttgaac cacgtccgga
     1141 gagcctctcc ggccagtgcg ccgggacggg agctgatcag gcccgccgcg gaccacttat
     1201 cgagattgta catcgggcag gtgtagcaga gctgaagcac gttctcgtgg actgcgtcga
     1261 cctctttgta ggcaggaacg atggagccgc cgacgaacgg cttgatttcc aggcggccgc
     1321 ccgaagcctc ggtgatggcc ttgcagagca tatcatgata aatctgggac ggatcggagg
     1381 caggaccgtg gccggaggaa cgccacacga tctttttcgg cgcctcggcg ccctcgacgg
     1441 aaacgggcgc aaaagcgcca aggacaaata ccgcgagcag caatacccaa caactttttc
     1501 tcagagca
//
LOCUS       DAOTIL010000761         1505 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminobacteriaceae bacterium isolate AMR_MDS_3026
            NODE_100047_length_1505_cov_2.637931, whole genome shotgun
            sequence.
ACCESSION   DAOTIL010000761 DAOTIL010000000
VERSION     DAOTIL010000761.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868050
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminobacteriaceae bacterium (wastewater metagenome)
  ORGANISM  Aminobacteriaceae bacterium
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae.
REFERENCE   1  (bases 1 to 1505)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1505)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:19:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,224
            CDSs (total)                      :: 3,198
            Genes (coding)                    :: 3,184
            CDSs (with protein)               :: 3,184
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1505
                     /organism="Aminobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_100047_length_1505_cov_2.637931"
                     /isolate="AMR_MDS_3026"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:3034609"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            198..1001
                     /locus_tag="P5201_12965"
     CDS             198..1001
                     /locus_tag="P5201_12965"
                     /inference="COORDINATES: protein motif:HMM:NF013247.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class IV"
                     /protein_id="HRV99501.1"
                     /translation="MNGVFLDGDQATLPVSDLALQRGVAVFDTVRSYDGRPFALKAHL
                     ERLSNSARLARIAEPLSTEEIGDIVREGLSRIEGDGLAKIFLTGGDVEEFGSFPSPRF
                     LALFSPVAPIPEEFFTKGIALSLLHEERQLFRIKSINYMNAYRNHLPGTFEALHCVDG
                     EITESATSSFFGVAKDNLITAPDHRVLRGITREILIDLARKEGYNVEHRCLTLDELPD
                     LTEAFITGSVKEVTPVTRIGDTIIGGGAPGAVTKRMYELFKESVPNHLG"
     gene            1062..>1505
                     /locus_tag="P5201_12970"
     CDS             1062..>1505
                     /locus_tag="P5201_12970"
                     /inference="COORDINATES: protein motif:HMM:NF015944.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LemA family protein"
                     /protein_id="HRV99502.1"
                     /translation="MGNGVYIVAAIAAGVLVYAVYIYNTLVRRKASVENGWSQIDVQL
                     KRRYDLIPNLVETVKGYAAHEREVFERIASLRSAALSATSPEQAAPLQNQLTGALRSL
                     FAVSENYPELKANQNFLALQEELASTENKIAFARQYYNDAVKAFNI"
BASE COUNT          321 a          406 c          438 g          340 t
ORIGIN      
        1 ggctaccaaa ataaaccgta cgaatgtgcg gcgttataca ttgaaagacc gcttgaacga
       61 gcggtctttt tttattcttc tttactgaaa caaccgattc ggcattgagc ttctcgcgtc
      121 ctgatgttag gatagtacta tcaaccgaac atatttttta cataatcgag gtgctttcca
      181 acatggtatt ttgtttcatg aacggggtgt ttctggatgg ggaccaggca acacttcccg
      241 tttccgacct tgcactgcag cgcggagtgg cggttttcga caccgtccga agctacgacg
      301 gccgtccttt cgctctcaag gcccatctgg aaaggctttc gaattcggcg cggctggcgc
      361 ggatcgcgga gcctctctct acggaagaga tcggagatat cgtccgggag gggttgtcgc
      421 gcatcgaagg ggatggcctg gcgaagatat tcctgaccgg cggggacgtc gaggagttcg
      481 gttcgtttcc ttcgccgcga ttcttagcgc tcttctcacc ggtcgctcct attccggagg
      541 agttttttac gaaggggatc gcgctttccc ttctgcacga ggagcgtcag cttttcagga
      601 taaagagcat caactacatg aacgcctaca ggaaccacct tccggggacg ttcgaggcgc
      661 tccactgcgt ggacggagag attacggagt ccgctacgag cagcttcttc ggcgtcgcga
      721 aagacaacct gatcacggct ccggaccatc gcgtgcttcg cggcattacg cgggagattc
      781 tcatagatct tgcgcgaaaa gaggggtaca acgtggagca ccgctgcctc acgctcgacg
      841 aactgcccga tctgacggag gccttcatca ccggaagcgt aaaagaggtg acgccggtga
      901 cgagaatagg cgacacgatt atcggcggcg gcgccccggg cgcggttacg aagcgcatgt
      961 acgaactctt caaggagagc gttcccaacc atctcggctg attcgcgctg gtgtataatg
     1021 tttttgttga agaacgtaat ccggaaggga ggctgtcgtc catgggaaat ggcgtctata
     1081 ttgtcgctgc gattgctgca ggtgttctcg tgtacgccgt ctatatctac aacacgcttg
     1141 tgcgcagaaa ggcgagcgtc gagaacggat ggagccagat cgacgtccag ttgaagcggc
     1201 ggtacgacct tatcccgaac cttgtggaga cggtgaaggg gtacgccgcg catgagcggg
     1261 aagttttcga gcgcatcgcc tctttacgaa gcgccgcgct ttccgcgacc tctcccgaac
     1321 aggccgctcc gttgcagaat cagctcaccg gcgcgctgcg ttcacttttt gcggtctcgg
     1381 agaactaccc cgagctgaag gcgaatcaga actttctcgc gcttcaggag gagctggcct
     1441 ccacggagaa taaaatcgcg ttcgcccgcc agtactacaa cgacgcggta aaggcgttca
     1501 acatc
//
LOCUS       DAOTIL010000762         1505 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminobacteriaceae bacterium isolate AMR_MDS_3026
            NODE_100052_length_1505_cov_2.566207, whole genome shotgun
            sequence.
ACCESSION   DAOTIL010000762 DAOTIL010000000
VERSION     DAOTIL010000762.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868050
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminobacteriaceae bacterium (wastewater metagenome)
  ORGANISM  Aminobacteriaceae bacterium
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae.
REFERENCE   1  (bases 1 to 1505)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1505)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:19:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,224
            CDSs (total)                      :: 3,198
            Genes (coding)                    :: 3,184
            CDSs (with protein)               :: 3,184
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1505
                     /organism="Aminobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_100052_length_1505_cov_2.566207"
                     /isolate="AMR_MDS_3026"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:3034609"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..603)
                     /locus_tag="P5201_12975"
     CDS             complement(<1..603)
                     /locus_tag="P5201_12975"
                     /EC_number="1.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013047443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HRV99503.1"
                     /translation="MQLVKGKSTVGFIGLGVMGHSMAGHVLRGGFDVLVYNRSKQKTL
                     DLLTQGAVWKDDIFSLAEKSDVVITMVGYPKDVEEVYLGERGIVAAARPGTVLIDMTT
                     SSPKLAERIASAAAEKGLFSLDAPVSGGDKGAREATLSIMIGGEETVFEDMLPLFQLM
                     GKNIVLQGGPGSGQHTKMANQIAIASNMMGVCEALAYAKKS"
     gene            764..>1505
                     /locus_tag="P5201_12980"
     CDS             764..>1505
                     /locus_tag="P5201_12980"
                     /EC_number="4.1.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006584325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004332 - fructose-bisphosphate aldolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I fructose-bisphosphate aldolase"
                     /protein_id="HRV99504.1"
                     /translation="MKTIERIEQLLGNEAKVLLEHKCATIDKSMLTLPGPDFVDRVTA
                     ISDRSIPVMRSMQTLFNSGRLAGTGYLSILPVDQGIEHSAGASFAPNPIYFDPENIVK
                     LAMEAGCNAVASTLGVLSSVARKYAHKIPFVLKINHNELLTYPNKHDQILFASVEQAR
                     DMGAVAVGATIYFGSDEATRQIQEISNAFEIAHELGMATILWCYLRNSAFKTAEKDYH
                     VSADLTGQGNHLGVTIGADIIKQKLPENN"
BASE COUNT          310 a          487 c          406 g          302 t
ORIGIN      
        1 cgattttttc gcgtacgcca aagcttcgca gacgcccatc atgttggagg caatagcgat
       61 ctgattggcc atcttcgtgt gctgaccgct tccgggaccg ccctgcagga cgatattctt
      121 ccccatcagc tggaagagcg ggagcatatc ctcgaaaacc gtctcctcgc cgccgatcat
      181 tatggagagc gtcgcttcgc gggctccctt atctcccccg gatacgggcg cgtcgagcga
      241 gaagagcccc ttctccgccg ccgccgaggc aatgcgctcc gcaagcttcg ggctcgacgt
      301 ggtcatgtcg ataaggactg tgccgggacg ggcggccgca acgattcccc tctcgccgag
      361 gtagacctcc tccacatcct tcgggtaccc caccatggtg atgacaacat cgctcttctc
      421 ggcgagagaa aaaatgtcgt ccttccagac ggccccctgt gtcagcaggt cgagtgtctt
      481 ttgcttggaa cggttgtaga cgaggacgtc gaaccctcct cgcagtacgt gtccggccat
      541 gctgtgcccc ataactccaa ggccgatgaa gccgaccgtc gacttcccct taaccagttg
      601 cattccccca cctccggcga atattcggcg ctttttttcg tttttttgtt ttcttcattc
      661 acagagcgcc ctttgtcggg tacaatctcc tcaaaagggt ttctcttcga gggatcgtag
      721 tcattgtacc ctcgttattt cagattgaaa aggaggcaga ttcatgaaga ctatcgaacg
      781 gattgaacaa ctgcttggaa acgaagcaaa agtgctcctg gagcacaagt gcgcaaccat
      841 cgacaagtcg atgctcaccc ttcccggccc cgactttgtc gaccgggtca ccgcgatctc
      901 cgaccgctcc atcccggtga tgcgctcaat gcagacgctc ttcaactcgg ggcgtctggc
      961 gggaacaggc tacctctcca tcctccccgt ggaccagggg atcgaacact cggccggagc
     1021 aagctttgcg ccgaacccga tctacttcga ccccgagaac atcgtcaagc tggcgatgga
     1081 ggcggggtgc aacgccgtgg cgagcacgct cggcgtcctc tcctccgtgg cgcggaagta
     1141 cgcccacaaa atccccttcg tgctgaagat caaccacaac gagctgctca cgtacccgaa
     1201 caagcacgac cagattctct tcgcgtcggt ggagcaggcc cgcgacatgg gcgccgtagc
     1261 tgtcggcgcg acgatctact tcggcagcga cgaggccacc cggcagatcc aggagatcag
     1321 caacgccttc gagatcgcgc atgagctcgg catggcgacc atcctgtggt gctatctgcg
     1381 caacagcgcc ttcaagaccg ccgagaagga ctaccatgta tcggccgacc tgacgggaca
     1441 ggggaaccac ctcggcgtga ccatcggcgc cgacatcatc aagcagaagc tcccggagaa
     1501 caacg
//
LOCUS       DAOTIL010000763         1503 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminobacteriaceae bacterium isolate AMR_MDS_3026
            NODE_100221_length_1503_cov_3.188536, whole genome shotgun
            sequence.
ACCESSION   DAOTIL010000763 DAOTIL010000000
VERSION     DAOTIL010000763.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868050
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminobacteriaceae bacterium (wastewater metagenome)
  ORGANISM  Aminobacteriaceae bacterium
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae.
REFERENCE   1  (bases 1 to 1503)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1503)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:19:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,224
            CDSs (total)                      :: 3,198
            Genes (coding)                    :: 3,184
            CDSs (with protein)               :: 3,184
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1503
                     /organism="Aminobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_100221_length_1503_cov_3.188536"
                     /isolate="AMR_MDS_3026"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:3034609"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..372)
                     /locus_tag="P5201_12985"
     CDS             complement(<1..372)
                     /locus_tag="P5201_12985"
                     /inference="COORDINATES: protein motif:HMM:NF018530.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YidE/YbjL duplication"
                     /protein_id="HRV99505.1"
                     /translation="MEFFESLLKNHLFLMSLTVALGILVGKISVRGINLGISGALFVG
                     LFIGAQGYSVPNEYFAWNLMFFVVAVGLLAAEDTARVVKRYGLKFILLSVAVTGAGAL
                     ATLAAGLLFSGSASPNMIAGTY"
     gene            complement(401..1234)
                     /locus_tag="P5201_12990"
     CDS             complement(401..1234)
                     /locus_tag="P5201_12990"
                     /inference="COORDINATES: protein motif:HMM:NF023568.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HRV99506.1"
                     /translation="MRFVLYLVAGYAVLVTIAFFFADKLLFFPHRELWATPSERGLPW
                     EDVRFEAADGTPLHGWYVPGAGRGPGGAAKATVLFCHGNAGNISHRIDSLAIFHRLGV
                     STFIFDYRGYGESEGTPSEQGVYSDVEGAWSWLTKERGVPPEQIILFGRSLGGAVAAY
                     AASRGEKFSPRGLILESTFTSLASVGQSHYFFLPMKLIVGNAFDTLSRLERIDCPVLV
                     ASSPEDEVVPGRHGRILFEAAREPKWFLPLKGDHNLGYMTTGRAYVDGLDAFFRNVLG
                     E"
     gene            complement(1265..>1503)
                     /locus_tag="P5201_12995"
     CDS             complement(1265..>1503)
                     /locus_tag="P5201_12995"
                     /inference="COORDINATES: protein motif:HMM:NF013717.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0043546 - molybdopterin cofactor binding
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="molybdopterin dinucleotide binding
                     domain-containing protein"
                     /protein_id="HRV99507.1"
                     /translation="SVHPLDAAFFDLKEGDEAVARGRRRGALRVVVREDASLGRGYCV
                     LPQGTEGVNSLTEPLASPGFGAPFAESWITLGKG"
BASE COUNT          307 a          511 c          446 g          239 t
ORIGIN      
        1 gtacgtcccc gcgatcatgt tcggcgacgc cgagccggag aagaggagtc ctgcggcgag
       61 cgtggcgagc gcccccgcgc ccgtcaccgc gacggagagc aggatgaact tgagcccgta
      121 gcgcttgacg acccgcgcgg tatcctcggc agccagcaag cccaccgcga ccacgaagaa
      181 catcaggttc cacgcgaaat attcgttcgg aacggagtac ccctgcgcgc cgatgaacag
      241 gccgacgaag agcgctcccg atatcccgag gttgatgcct ctgacgctga tcttcccgac
      301 gagaatgccc aatgcgaccg tcaggctcat caggaagagg tgattcttga gaagggactc
      361 gaaaaactcc actggaaaca tctccttatc tgttgtgaac ttactcgccg aggacgttcc
      421 ggaagaaagc gtccaatccg tcgacgtacg cccgccccgt cgtcatatat ccgaggttgt
      481 ggtctccttt cagagggaga aaccacttcg gctcgcgcgc cgcctcgaag agaattctgc
      541 cgtgtctgcc ggggaccact tcgtcctccg ggctcgacgc cacaagaacc gggcagtcta
      601 tccgctcgag tcgggagagg gtgtcgaacg cgttccccac gatcagcttc atcggaagaa
      661 agaagtagtg actctgcccg accgaggcaa gcgaggtaaa ggtagactcc agaatcagcc
      721 ctcgcgggga gaacttttcg ccgcgagatg ccgcgtacgc cgccaccgcg ccgccgagcg
      781 acctgccgaa gagaatgatc tgctcgggag gaacgccgcg ctccttcgtc agccagctcc
      841 acgcaccttc aacgtcgctg taaaccccct gttcggaggg cgttccctcg ctctctccgt
      901 accccctgta gtcgaagatg aaggtggaga cgccgagccg gtgaaagatc gccagcgaat
      961 cgatccggtg cgagatattt cccgcgttgc cgtggcagaa gagcaccgtc gccttcgccg
     1021 cgccgcccgg cccccgccct gcgcccggca cgtaccagcc gtggagcggg gttccgtctg
     1081 cggcctcgaa gcgcacatct tcccacggaa gcccccgttc ggagggcgtc gcccaaagct
     1141 cgcggtgcgg aaagaagagc agtttgtccg cgaagaaaaa agcgatcgtc acaagcaccg
     1201 catatcctgc tacaagatag agtacaaaac gcatgcgcac gggaaacctc cttaccggga
     1261 cctgctaccc cttgccgagc gtgatccagc tttcggcgaa cggcgcgccg aaaccggggc
     1321 tcgccagcgg ttcggtcagc gagttgacgc cctccgtccc ctgcgggagc acgcagtacc
     1381 cccgcccgag cgaagcgtcc tcccggacca cgacgcgaag cgctccccgt cgtctgccgc
     1441 gcgcgacggc ctcgtcgccc tccttcaggt caaagaacgc cgcgtccagc gggtgcaccg
     1501 aga
//
LOCUS       DAOTIL010000764         1502 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminobacteriaceae bacterium isolate AMR_MDS_3026
            NODE_100302_length_1502_cov_3.850726, whole genome shotgun
            sequence.
ACCESSION   DAOTIL010000764 DAOTIL010000000
VERSION     DAOTIL010000764.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868050
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminobacteriaceae bacterium (wastewater metagenome)
  ORGANISM  Aminobacteriaceae bacterium
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae.
REFERENCE   1  (bases 1 to 1502)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1502)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:19:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,224
            CDSs (total)                      :: 3,198
            Genes (coding)                    :: 3,184
            CDSs (with protein)               :: 3,184
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1502
                     /organism="Aminobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_100302_length_1502_cov_3.850726"
                     /isolate="AMR_MDS_3026"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:3034609"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..518
                     /locus_tag="P5201_13000"
     CDS             <1..518
                     /locus_tag="P5201_13000"
                     /inference="COORDINATES: protein motif:HMM:NF013618.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="lytic transglycosylase domain-containing
                     protein"
                     /protein_id="HRV99508.1"
                     /translation="VWAIMRQESAFNPNATSWVGAGGLMQLMPGTARSEAKALEMKKY
                     SVYNPEQNIILGSAHIARLLRSFGDVEVALAAYNAGGGNARKWLDGRKDVPLDEWIES
                     VRFEETNDYVQKVMANLHIYRTLYAPSRLSASDAAAPGDVPDEDAEPGSAGSEPEESD
                     DAPVPADDGGK"
     gene            complement(553..>1502)
                     /locus_tag="P5201_13005"
     CDS             complement(553..>1502)
                     /locus_tag="P5201_13005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013047776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HRV99509.1"
                     /translation="KEAAMASLRELLPEIDMEMEEIPYDRLLAMDVRMDHFLDAFREV
                     DPSAIREVFVEIPDVSWRDIGGLEDIKEELHDAVQWPLEHADIFEKFAISPPKGIMLH
                     GASGTGKTLLAKALAHESGVNFISVKGPALMSKYVGESERAIREVFRTAKQAAPSILY
                     FDEIESLVPVRGKDSGSGASFTERVISQFLAEMSGIEELKGVVVLATTNRLDLVDPSL
                     LSSGRFDLLLELPKPDAAAREKIFEIHLRKKPLSAEIRIPTLARLTDGRSGGDIAFVC
                     RKASMMAIRDHIKGGGEAEPLLEWRHFETALKELKNVLS"
BASE COUNT          318 a          430 c          450 g          304 t
ORIGIN      
        1 tcgtctgggc gatcatgcgt caggagagcg ccttcaaccc gaacgcgaca agctgggtcg
       61 gcgcaggcgg cctgatgcag ctcatgccag gcacggcgcg aagcgaggcg aaagcgctgg
      121 agatgaaaaa gtatagcgtc tacaacccgg agcagaacat catcctgggc agtgcgcaca
      181 ttgcgcgtct tctgcgctct ttcggcgacg ttgaagttgc tttagcggca tacaacgcag
      241 gaggaggaaa cgcccggaaa tggcttgacg gtcggaagga cgtgccgctc gacgagtgga
      301 ttgaatccgt ccgtttcgag gaaaccaacg actatgtgca aaaggtgatg gcgaacctgc
      361 atatctatcg cacgctgtat gcgccttcac gtctctctgc ctcggacgcg gccgcgccgg
      421 gagacgttcc cgacgaggat gcggagccgg ggagcgccgg atcggagccg gaggagagcg
      481 acgacgcccc cgttcctgct gatgacggag gaaaataaag aagggcggac cggataacgg
      541 tccgcccttc ttctatgata aaacgttctt tagctctttg agcgcggttt cgaaatgccg
      601 ccattcgaga agaggttcgg cttcgccgcc gcctttgatg tgatcacgta tcgccatcat
      661 cgacgccttg cggcagacga aggcgatgtc gcctcctgaa cgcccgtcgg tcagcctcgc
      721 cagcgtcgga atacgaatct ccgcagacag cggttttttt cgaagatgaa tctcgaagat
      781 cttctccctg gcagcagcgt cgggcttcgg tagttcaagg aggaggtcga atctgccgct
      841 ggagagaagc gaaggatcca caagatccag acggttcgtt gttgcaagga ccaccacgcc
      901 tttcagctct tctatgccgc tcatctctgc aaggaactga ctgataactc gctctgtgaa
      961 gctcgctccg ctgccgctgt ccttcccccg gaccgggaca agagattcaa tctcgtcgaa
     1021 gtagaggatg gaaggagcag cctgcttcgc ggtacggaag acctcgcgga tggcgcgttc
     1081 gctctcgccg acgtatttcg acatgagcgc cggtcccttc acggagatga agttcacgcc
     1141 gctctcatgg gcgagcgcct ttgcgagaag cgttttgccc gttcccgaag ctccatgaag
     1201 catgatacct ttgggaggcg agatggcgaa tttctcaaag atgtcggcgt gttccagagg
     1261 ccactggacc gcgtcgtgca gctcttcttt aatatcttcc aaaccgccga tatcgcgcca
     1321 cgatacatcg gggatctcaa cgaaaacctc ccgtatcgcc gaaggatcga cctccctgaa
     1381 cgcgtcgagg aagtggtcca tccgtacgtc catggcaaga aggcggtcgt agggaatctc
     1441 ttccatctcc atgtcgatct cggggagcag ttcgcgaagg ctggccatcg cggcctcctt
     1501 gg
//
LOCUS       DAOTIL010000765         1501 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminobacteriaceae bacterium isolate AMR_MDS_3026
            NODE_100419_length_1501_cov_3.327801, whole genome shotgun
            sequence.
ACCESSION   DAOTIL010000765 DAOTIL010000000
VERSION     DAOTIL010000765.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868050
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminobacteriaceae bacterium (wastewater metagenome)
  ORGANISM  Aminobacteriaceae bacterium
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae.
REFERENCE   1  (bases 1 to 1501)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1501)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:19:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,224
            CDSs (total)                      :: 3,198
            Genes (coding)                    :: 3,184
            CDSs (with protein)               :: 3,184
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1501
                     /organism="Aminobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_100419_length_1501_cov_3.327801"
                     /isolate="AMR_MDS_3026"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:3034609"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1249)
                     /locus_tag="P5201_13010"
     CDS             complement(<1..1249)
                     /locus_tag="P5201_13010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005661842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ni/Fe hydrogenase subunit alpha"
                     /protein_id="HRV99510.1"
                     /translation="MENKKNTIVINPITRIEGHGKITVDLTDDGAVDNVRFHVTQYRG
                     FEVFSKGRDFREMPVITPRICGICPVSHHLASAKACDAILGVTPPAPAAKLREVMHMG
                     QFIQSHALSFFHLSSPDLLFGFDADPAVRNVAGLAKEYPELAVAGIGMRKFGQEVIKT
                     LGGKKVHPWHSIPGGVNRSLRAEEKDAMLAGLPAHKENLRKALSLVKNYLAENAELAA
                     RFATLETSYLGLVKDGNLELYDGRIRLRSPRGRITDEFDDKDYCANIGEHVEEWSYLK
                     FPFYRPLGFPHGSYRVGPLGRLNACEDIPTPEASAELAAFRKTTEDGIVHYTLYYHYA
                     RLIEILYAMERLEELLLDPDVLKSDLHITGEVSNREGIGVVEAPRGTLIHHYEVDENG
                     VIERVNLIVATGHNNYAMNRGVEQ"
     gene            complement(1264..>1501)
                     /locus_tag="P5201_13015"
     CDS             complement(1264..>1501)
                     /locus_tag="P5201_13015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014163746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="NADP oxidoreductase"
                     /protein_id="HRV99511.1"
                     /translation="QVLREGYVETVSTVNPEGVIPGKDLPALLPYAIPVDRAVKVDVY
                     VPGCPPDADTIYYVFSEILEGRIPSVPTDIMRYD"
BASE COUNT          274 a          446 c          444 g          337 t
ORIGIN      
        1 cctgctctac acccctgttc attgcgtaat tattatgccc ggtggcaacg atgagattca
       61 cccgctcgat cactccgttc tcatctacct catagtgatg gatgagcgta ccgcgaggcg
      121 cctcgacgac tccgattccc tcgcgattgg agacctctcc cgtaatatgc agatcgcttt
      181 tcagtacgtc agggtcgagc agcaattcct ccagccgctc catcgcgtac agaatctcga
      241 tgaggcgagc gtagtggtag tagagcgtat agtggacaat gccgtcttcg gtggttttcc
      301 tgaaagccgc cagttcggcg gaggcctccg gcgtcgggat gtcctcgcag gcattcaaac
      361 gtccgagagg gccgaccctg taagaaccgt gcggaaaccc gagcggcctg tagaacggaa
      421 acttgaggta gctccactct tcgacgtgtt cgccgatatt tgcgcagtaa tccttgtcgt
      481 cgaactcgtc ggtgatgcgt cccctggggc ttcgcaagcg gatacgtccg tcgtacagtt
      541 caaggttgcc gtctttcacg aggcccaggt aggaggtctc cagcgtggcg aagcgcgcgg
      601 caagctcggc attctcggcc aggtagttct tgaccagcga gagcgccttt ctcaggttct
      661 ccttgtgcgc cggaagccct gcgagcatgg cgtccttttc ctccgcgcgg aggctcctgt
      721 tgactcctcc tgggatgctg tgccacggat ggaccttctt gcctccgagg gttttgatga
      781 cctcctgtcc gaactttctc atgccgatcc cggccacggc gagttcaggg tactccttgg
      841 ccagcccggc tacatttcgt acagccgggt cggcatcgaa cccgaaaagg agatcgggac
      901 tcgagaggtg gaagaaggag agcgcatggg actggatgaa ctgccccatg tgcatcacct
      961 cgcggagctt cgccgccgga gcgggaggcg tcacgccgag gattgcgtcg catgcttttg
     1021 cggacgcaag atggtggctc accgggcaga taccgcagat gcgcggcgtg ataacgggca
     1081 tctcgcggaa atcgcgaccc tttgaaaaaa cttcgaaccc tctgtactgc gtcacgtgga
     1141 agcggacatt gtccaccgct ccgtcgtccg tgaggtccac ggtgattttg ccatggccct
     1201 cgatgcgggt aatgggattg atcactatcg tatttttctt gttctccatt cttcggtcct
     1261 ccgctaatcg tagcgcatga tgtccgtcgg gacggaggga atccgtcctt ccagaatctc
     1321 gctgaagaca taatagatgg tgtcggcgtc gggagggcac cccggaacgt agacgtccac
     1381 cttcaccgcc cggtccacgg gaattgcgta cgggagcagt gcggggaggt ctttcccggg
     1441 gatgacccct tcggggttca ccgtgctgac cgtctccacg tacccttccc tgagcacctg
     1501 c
//
LOCUS       DAOTIL010000766         1501 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminobacteriaceae bacterium isolate AMR_MDS_3026
            NODE_100440_length_1501_cov_2.634855, whole genome shotgun
            sequence.
ACCESSION   DAOTIL010000766 DAOTIL010000000
VERSION     DAOTIL010000766.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868050
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminobacteriaceae bacterium (wastewater metagenome)
  ORGANISM  Aminobacteriaceae bacterium
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae.
REFERENCE   1  (bases 1 to 1501)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1501)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:19:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,224
            CDSs (total)                      :: 3,198
            Genes (coding)                    :: 3,184
            CDSs (with protein)               :: 3,184
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1501
                     /organism="Aminobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_100440_length_1501_cov_2.634855"
                     /isolate="AMR_MDS_3026"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:3034609"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(289..606)
                     /locus_tag="P5201_13020"
     CDS             complement(289..606)
                     /locus_tag="P5201_13020"
                     /inference="COORDINATES: protein motif:HMM:NF027117.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system mRNA interferase
                     toxin, RelE/StbE family"
                     /protein_id="HRV99512.1"
                     /translation="MSFPVEFLPEAADDYKKLDGSVRKDVNKKIDELAENPFLGERLG
                     HKFSIDLTGFLKIYVHGKKYRIVYRLIEPERIEIIEIWGIGKREKEKMYKLIGERVKK
                     RTK"
     gene            complement(606..878)
                     /locus_tag="P5201_13025"
     CDS             complement(606..878)
                     /locus_tag="P5201_13025"
                     /inference="COORDINATES: protein motif:HMM:NF014643.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system Phd/YefM family
                     antitoxin"
                     /protein_id="HRV99513.1"
                     /translation="MLVETRRIMPITRLQRELTQTVRDISETGEPVYIVKNNTMEAVM
                     VPFEEYEYLAALEEMFEYYEIDDVVQKRLGAYDPGKNIRWSELRED"
     gene            961..1089
                     /locus_tag="P5201_13030"
     CDS             961..1089
                     /locus_tag="P5201_13030"
                     /inference="COORDINATES: protein motif:HMM:NF018476.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA];
                     GO_process: GO:0006401 - RNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system YoeB family
                     toxin"
                     /protein_id="HRV99514.1"
                     /translation="MRRHKQDKRMVERIDRLIREIQREPFAEVGKPEALKHAPAGY"
     gene            complement(1120..>1501)
                     /locus_tag="P5201_13035"
     CDS             complement(1120..>1501)
                     /locus_tag="P5201_13035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013048972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="macro domain-containing protein"
                     /protein_id="HRV99515.1"
                     /translation="GPELLEECRKLGGRDTGDAKLTKGYRLPAHYVIHTVGPVWHGGK
                     SGEPGLLASCYRRSLEIAQSAGAKSIAFPSISTGVYGYPADQAAKIAVDTVRETLKTC
                     PGIEKVVFCCFSEKDRAVYEGVIG"
BASE COUNT          330 a          424 c          360 g          387 t
ORIGIN      
        1 ggccactttt gtcgagaggc tttacaaaaa tgactgtgcc ggataagccg aacagcaagt
       61 tgcaaaaata ccgtctgacg gacagcggta gagcggctat ccggtaacac gcgcgataac
      121 gcggctatcc aatgaagctt ccggtatcaa gacgttcgct gttcagctca ggagagtgtt
      181 tcaaagcccc tcatcctact actctcctct ttgcatgcgc aggactaccc ggtctgtacg
      241 aattcgggcc gaaaatcatg gcggcgaaca actccttgac ggcgggccct acttcgtacg
      301 cttctttact ctttctccga tcaatttata catcttctct ttttcccttt ttccaattcc
      361 ccaaatttct attatttcaa tcctctccgg ctcaatcagg cgatagacaa ttctgtattt
      421 ctttccatga acatagatct tcaggaagcc cgtcaagtcg atcgaaaact tatggcccaa
      481 ccgctctcct aaaaacgggt tttcggccaa ctcgtcgatt tttttattca catcttttcg
      541 tacgcttcca tccaatttct tataatcatc cgccgcttca ggtaaaaact cgaccgggaa
      601 cgacatcagt cttctcttaa ttccgaccaa cgtatatttt tgccaggatc ataagcgccc
      661 aaacgctttt ggacgacatc gtctatctca tagtactcga acatctcctc aagagccgcc
      721 aaatactcat actcctcaaa aggcaccatt acggcctcca tggtgttgtt ctttacgatg
      781 tataccggtt cgccggtttc ggaaatatct ctgacggttt gagtcagttc tctttgcaga
      841 cgagttatcg gcattatacg gcgcgtttcc accaacatgt cgaccacctc caatgttgat
      901 tataatacat actttagcat gtagtcaaat acatactatt cacgcccgtg agaggactgt
      961 ttgcgccggc ataaacagga caagcggatg gtcgagcgga tcgacaggct gatcagggag
     1021 attcagcggg agccgttcgc ggaagtcggg aagcccgaag ccctgaagca tgctccggcc
     1081 ggttattgag tccgatccgt cgcattactt caaaacccgt tacccgatca ccccctcgta
     1141 caccgcccgg tccttctcgg agaaacagca gaagacgact ttctcaatcc ccgggcaggt
     1201 tttgagcgtc tcgcgtactg tatccacggc gatcttcgcg gcttggtccg cggggtagcc
     1261 gtagacgccg gtgctgatac tcgggaaggc gatcgacttg gctcccgcgc tttgggctat
     1321 ttccagggag cggcggtagc aggaggcgag gaggccgggt tcgccgctct ttcctccgtg
     1381 ccagacggga cctactgtgt ggatgacgta gtgtgcggga aggcggtacc ctttggtgag
     1441 tttggcgtcg ccggtgtcgc ggccgcccag ctttcggcac tcttcgagca gttcggggcc
     1501 c
//
LOCUS       DAOTIL010000767         1500 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminobacteriaceae bacterium isolate AMR_MDS_3026
            NODE_100517_length_1500_cov_3.032526, whole genome shotgun
            sequence.
ACCESSION   DAOTIL010000767 DAOTIL010000000
VERSION     DAOTIL010000767.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868050
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminobacteriaceae bacterium (wastewater metagenome)
  ORGANISM  Aminobacteriaceae bacterium
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae.
REFERENCE   1  (bases 1 to 1500)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1500)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:19:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,224
            CDSs (total)                      :: 3,198
            Genes (coding)                    :: 3,184
            CDSs (with protein)               :: 3,184
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1500
                     /organism="Aminobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_100517_length_1500_cov_3.032526"
                     /isolate="AMR_MDS_3026"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:3034609"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..936)
                     /locus_tag="P5201_13040"
     CDS             complement(<1..936)
                     /locus_tag="P5201_13040"
                     /inference="COORDINATES: protein motif:HMM:NF012706.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HRV99516.1"
                     /translation="MKGKFSSKVAVLAVAAVVLVAMFAVPALAKDTLTVANIYDARSL
                     DPIIQNEVAASNAICHINETLIELTDDGMPTPLLAETFEQVDSRTYKFVLRQGVKFHN
                     GDPFTAADVLYTIERAKSPLGSAIKQYPEIIESAEAPDDHTVIIKIKYPFTPFWGTIA
                     HTSLAIVNKRAVEEKGDMYPLEPVGTGPFQFKEWVKNDRLTLERFEDYWGTKPAYKTL
                     VIRSIPESVNRTIELETGAIDIAFRIPVSDVSRVKENKDLDVIGGFEHSVTYMGFNCE
                     KEPWSNPKVREAIKLALDINGARHAVFRGTSLTPVS"
     gene            1207..>1500
                     /locus_tag="P5201_13045"
     CDS             1207..>1500
                     /locus_tag="P5201_13045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008520207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HRV99517.1"
                     /translation="MITRSLIELVFSAASMERWNDHPRPAVFTELGKQAHKMIMAWVI
                     ARYESETRGVAVDWTALIEGGIFEFLHRVVLTDIKPPVFHKLMQNEEQRKKLNS"
BASE COUNT          332 a          399 c          403 g          366 t
ORIGIN      
        1 cgagacggga gtcaaactgg ttcctctgaa gaccgcgtgg cgggcgccgt tgatatcgag
       61 cgccagtttg atggcctcgc ggactttcgg gttgctccac ggctctttct cgcagttgaa
      121 gcccatatac gtcaccgaat gctcgaagcc gccgatcacg tcgaggtcct tgttctcttt
      181 cacgcgggag acgtcggaaa ccggaattct gaaggcaatg tcgattgcgc ctgtctcaag
      241 ctcaatggtg cggttgacgc tctcgggaat ggaacggatt acgagggtct tatacgcggg
      301 tttcgttccc caataatctt cgaagcgctc aagagtcaaa cggtcgttct tcacccactc
      361 tttgaactgg aagggaccgg tgcctaccgg ctcgagcggg tacatgtcgc ccttctcctc
      421 aacggcgcgt ttgttcacga tcgcgaggga ggtatgggct atggttcccc agaagggagt
      481 gaacggatac ttgatcttga tgatgacggt atggtcgtcc ggagcttcag cgctctcgat
      541 gatttcggga tactgcttga tcgccgagcc gagagggctc ttcgcccgct caatggtgta
      601 gaggacgtca gccgcagtga agggatcacc gttgtggaac ttcacgccct ggcgcagaac
      661 gaatttgtag gtcctggaat cgacctgctc gaaggtctcc gcaagcaacg gagttggcat
      721 tccatcgtcg gtaagctcaa tgagcgtctc gttgatgtgg catatcgcgt tgcttgcagc
      781 cacctcgttc tggataatcg ggtcgagaga gcgcgcgtcg taaatgttgg cgacggtgag
      841 cgtatccttc gccagcgccg gaaccgcgaa catggccaca agaaccactg cggctacggc
      901 aagaaccgca accttggaac tgaactttcc tttcatactt tcatctcctt tgtggatttt
      961 tttttgcatc agaagaaaaa agaaactttt gttatttatt ttccccaaga gtttcttctt
     1021 cagtgatgcc cttgaattat tatgcacgga ccgacatatt gtcaagaagt attatgcata
     1081 cataattgca cgcgcttttt gaagcaatct tggccaaagg ttgtataatt attttattca
     1141 aaccctccgc acgattacac tctctgcgga gaagatattt gaattacttt tcgggaggcc
     1201 cttctaatga tcacacgatc gctcatcgaa ctcgtcttct ccgccgccag tatggaacgc
     1261 tggaacgatc atccgcggcc ggcagtcttc acggagctcg gcaaacaggc gcacaagatg
     1321 atcatggcgt gggtcatcgc ccgctatgaa tcggaaacgc gcggcgtcgc ggtcgactgg
     1381 acggccctca tcgagggcgg catcttcgaa ttcctccaca gggtcgtgct caccgacatc
     1441 aagccccccg tcttccacaa gctgatgcag aacgaggagc agcgaaaaaa actgaactca
//
LOCUS       DAOTIL010000768         1493 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminobacteriaceae bacterium isolate AMR_MDS_3026
            NODE_101136_length_1493_cov_3.441586, whole genome shotgun
            sequence.
ACCESSION   DAOTIL010000768 DAOTIL010000000
VERSION     DAOTIL010000768.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868050
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminobacteriaceae bacterium (wastewater metagenome)
  ORGANISM  Aminobacteriaceae bacterium
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae.
REFERENCE   1  (bases 1 to 1493)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1493)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:19:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,224
            CDSs (total)                      :: 3,198
            Genes (coding)                    :: 3,184
            CDSs (with protein)               :: 3,184
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1493
                     /organism="Aminobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_101136_length_1493_cov_3.441586"
                     /isolate="AMR_MDS_3026"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:3034609"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..811)
                     /gene="pstC"
                     /locus_tag="P5201_13050"
     CDS             complement(<1..811)
                     /gene="pstC"
                     /locus_tag="P5201_13050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005659716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease subunit PstC"
                     /protein_id="HRV99518.1"
                     /translation="MKDRLPGIVVKGMSSAGIFIMIFILYFLFREGIPVLKAVTLKDL
                     FFGDLWYPAENPPVLGMFPLIVGTLAVTAASSLLALPFSLLIAVFVSEVAPRSVRELL
                     KPVLELLGFFPSIVLGFIGMVVLAPWLQETFDMLSGLNLLNASVLLGVLTVPIVSSLA
                     EEALSAVPADLRDASYALGATRFETTRKVVVPSALPGILSASLLGVMRALGETMVVLM
                     AAGGAAIIPLSIFDPVRPLTSTIAAEMGETPVGSPHYAALFFGGIILLLMTL"
     gene            complement(926..>1493)
                     /locus_tag="P5201_13055"
     CDS             complement(926..>1493)
                     /locus_tag="P5201_13055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008712510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006817 - phosphate ion transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="phosphate ABC transporter substrate-binding
                     protein"
                     /protein_id="HRV99519.1"
                     /translation="EVKAAVEKGTYPVPFAVAMDALLPIVHPENPVSDLSIEQLRDIY
                     AGKIINWKDVGGDDKGIAVVGRDTSSGTYETWQEIIMKKERVSQRALVVASSGAMLTT
                     VAKNKYAIGYDGIGYVNNTVKPLMVGGKKGSAETALDGTYPIARFLYMFTKGWPTGEI
                     MKFINFVLSDEGQKIVAETGFVPLRVLK"
BASE COUNT          311 a          490 c          430 g          262 t
ORIGIN      
        1 cgagcgtcat cagcagcaga atgataccgc cgaagaagag cgcggcatag tgcggcgacc
       61 ctaccggagt ctcccccatc tccgccgcga tggtggaggt cagcggacgc accgggtcaa
      121 aaatcgagag cgggatgatc gccgcgccgc ccgccgccat cagcaccacc atcgtctccc
      181 ccagcgcgcg catcacccca agcagcgacg cgctcaaaat accggggagc gccgagggca
      241 cgaccacctt ccgcgtcgtc tcgaaccggg tcgcgccgag cgcgtaggag gcgtcccgca
      301 gatcggcggg caccgcgctc agcgcttcct cggcgagcga actcacgatc ggcacggtca
      361 gcaccccgag caataccgag gcgttcagca gattcagccc cgagagcatg tcgaacgtct
      421 cttgcagcca gggcgccagc accaccatcc cgatgaaccc cagcacgatg gagggaaaaa
      481 agcccagcag ctcaagcacc ggcttcagca gctcgcgtac agatctgggc gccacctcgg
      541 agacgaaaac cgctatcagc aaactgaacg gaagcgccag cagcgaggag gccgccgtca
      601 ccgcaagcgt tcccacgata agaggaaaca tccccagtac cggaggattc tccgccggat
      661 accacagatc gccgaaaaac agatccttca gcgtcaccgc tttcagcacg ggaataccct
      721 ctctgaacag gaaatagagg atgaagatca tgatgaagat ccccgcgctc gacattccct
      781 tgactacaat tcccggaagc ctgtctttca tgaaaaaacc tctttcgttc gtcgaattcg
      841 tcgccctcgg cgggcccgga aaaagccccc tccccgtgag gggaaggggt acggctggag
      901 gaactctctg gatgagtcgt cgatgttatt tcagcactct caggggaacg aatccggttt
      961 cggccactat cttctgcccc tcgtcgctca ggacgaagtt gatgaacttc atgatttcgc
     1021 cggtgggcca gcccttcgtg aacatgtaga ggaagcgtgc gatggggtac gtgccgtcaa
     1081 gcgccgtctc cgccgagcct ttcttgccgc ccaccatcag gggcttcacg gtgttgttga
     1141 cgtagccgat gccgtcgtag cctatcgcgt acttgttctt ggccacggtg gtgagcatgg
     1201 ctccggagga ggcaaccaca agcgcgcgct gggaaacacg ttccttcttc atgatgatct
     1261 cctgccacgt ctcgtaggtg ccggagctgg tgtcgcggcc cacgaccgcg attcccttgt
     1321 cgtcgccgcc gacgtctttc cagttgatga tcttgccggc gtagatgtcg cgaagctgct
     1381 cgatgctcag gtcgctcacg gggttctccg ggtgcacgat gggaagcagc gcgtccatgg
     1441 ccaccgcgaa gggcaccgga tacgttccct tctccacagc cgccttcact tcg
//
LOCUS       DAOTIL010000769         1492 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminobacteriaceae bacterium isolate AMR_MDS_3026
            NODE_101211_length_1492_cov_4.121086, whole genome shotgun
            sequence.
ACCESSION   DAOTIL010000769 DAOTIL010000000
VERSION     DAOTIL010000769.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868050
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminobacteriaceae bacterium (wastewater metagenome)
  ORGANISM  Aminobacteriaceae bacterium
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae.
REFERENCE   1  (bases 1 to 1492)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1492)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:19:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,224
            CDSs (total)                      :: 3,198
            Genes (coding)                    :: 3,184
            CDSs (with protein)               :: 3,184
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1492
                     /organism="Aminobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_101211_length_1492_cov_4.121086"
                     /isolate="AMR_MDS_3026"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:3034609"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..566
                     /locus_tag="P5201_13060"
     CDS             <1..566
                     /locus_tag="P5201_13060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013048404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent enzyme"
                     /protein_id="HRV99520.1"
                     /translation="YMDTDYCEAPHGRAPAVATGLKTVRPDKIVYTYQGDGDLASIGM
                     GEIIHAANRGQNITVIFINNAIYGMTGGQMAPTTLIGQRASTCPLGRDVKLNGYPIRV
                     SELLAALPAPGYVTRVTVAQPKYIIKAKQAVKQAFQNQLDGKGFSLVEILSSCPTNWG
                     MRPVEAFDWLVNTMMPYYPLGTFKDFD"
     gene            579..1136
                     /locus_tag="P5201_13065"
     CDS             579..1136
                     /locus_tag="P5201_13065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014806666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoacid:acceptor oxidoreductase family
                     protein"
                     /protein_id="HRV99521.1"
                     /translation="MSFFKTLLAAGFGGQGVMVLGQLVAYTGIEEGRHVTWIPSYGPE
                     MRGGTANCGVVLSDDEIGSPVVDKADVLVVMNQPSLSKFIDRLKPGGVLVYNSNLVEY
                     DNSRKDIRVVALPATTLAEELGSDKITNVVILGAVVESSGIVADAVCVETIKEKLGKK
                     KPQFLPMNLGAYEKGKEVARECLKA"
     gene            1121..>1492
                     /locus_tag="P5201_13070"
     CDS             1121..>1492
                     /locus_tag="P5201_13070"
                     /EC_number="3.6.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012514.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HRV99522.1"
                     /translation="MSEGVREKTLAQKTVYSGRILNLRVDDVELPGGKRAIREVVEHK
                     PAVGILPVMEDGTVLLIRQYRYAVGEDMLEIPAGIVEGNEPFDDAARRELQEEVGYFP
                     EHLREIGRIYNSPGFSTELMAL"
BASE COUNT          324 a          404 c          472 g          292 t
ORIGIN      
        1 actacatgga caccgactac tgcgaagcgc cccacgggcg cgctcctgca gtggcgaccg
       61 gattaaagac cgtccgtccc gacaagatcg tgtacaccta ccagggcgac ggcgaccttg
      121 cctcgatagg catgggcgag atcattcatg ccgcaaacag agggcagaac atcacggtca
      181 tcttcatcaa caacgcgatt tacggtatga ccggcggtca gatggcgccg acgacgctta
      241 tcgggcagag agcctccaca tgtccgcttg gacgcgacgt caagctcaac ggctacccga
      301 tccgcgtgag cgaacttctg gcggcgcttc ctgcgcccgg ctacgtgacc cgcgtgacgg
      361 tggcgcagcc aaagtacatc atcaaggcga agcaggctgt gaaacaggcc ttccagaacc
      421 agttggacgg gaaggggttc tccctcgtcg agatactctc ctcctgcccc acgaactggg
      481 gaatgcgtcc tgtggaagcc ttcgactggc ttgtcaacac gatgatgccc tactatcccc
      541 ttggtacgtt caaggatttc gactagggag ggaagagcat gtcatttttc aagacgcttc
      601 tcgcggccgg attcggcggt cagggagtca tggttttggg acagctcgtg gcgtatacgg
      661 ggatcgaaga gggcagacat gtaacgtgga ttccctcgta cggtccggag atgcgcggcg
      721 gaacggcgaa ttgcggcgtg gtgctttcgg acgacgagat cggttcgccc gttgtcgaca
      781 aggcggacgt ccttgttgtg atgaaccagc cgtcgctttc caagttcatc gacaggctga
      841 agccgggcgg cgtcctggtg tacaacagca atctcgtcga atacgacaac tcccgtaagg
      901 atattcgcgt cgtcgccctt cccgcgacca ccctcgccga ggagctgggc agcgacaaga
      961 tcacgaacgt cgttattctg ggcgcggtcg tggagtcgag cggtattgtc gccgacgccg
     1021 tgtgcgtgga gacgatcaag gagaagctgg gtaaaaagaa gccgcagttc ctcccgatga
     1081 acctcggcgc gtacgaaaag ggaaaagagg ttgccaggga atgtctgaag gcgtgaggga
     1141 gaagaccctt gcgcagaaga ccgtctattc cggaagaata ctgaacctcc gtgtggacga
     1201 tgttgagctt cccgggggga agcgggcgat ccgcgaggtc gtggagcaca agcctgctgt
     1261 tggtatcctt ccagtcatgg aagacgggac cgttcttttg atacggcagt accgttacgc
     1321 tgtcggggag gatatgctcg aaattcccgc aggaattgtc gagggaaacg agcctttcga
     1381 cgatgccgct cgaagagagc ttcaggaaga agtgggctac tttccggagc atttgcggga
     1441 gatcggacgt atctacaact ctccggggtt cagtacggag ctgatggcgc tg
//
LOCUS       DAOTIL010000770         1491 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminobacteriaceae bacterium isolate AMR_MDS_3026
            NODE_101323_length_1491_cov_3.243733, whole genome shotgun
            sequence.
ACCESSION   DAOTIL010000770 DAOTIL010000000
VERSION     DAOTIL010000770.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868050
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminobacteriaceae bacterium (wastewater metagenome)
  ORGANISM  Aminobacteriaceae bacterium
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae.
REFERENCE   1  (bases 1 to 1491)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1491)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:19:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,224
            CDSs (total)                      :: 3,198
            Genes (coding)                    :: 3,184
            CDSs (with protein)               :: 3,184
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1491
                     /organism="Aminobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_101323_length_1491_cov_3.243733"
                     /isolate="AMR_MDS_3026"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:3034609"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            223..>1491
                     /locus_tag="P5201_13075"
     CDS             223..>1491
                     /locus_tag="P5201_13075"
                     /inference="COORDINATES: protein motif:HMM:NF013079.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucyl aminopeptidase family protein"
                     /protein_id="HRV99523.1"
                     /translation="MKILTAPSNGGFVREGALALFVFSKESVSGGLLDEHFADMAARL
                     MEEPSFRGKKGKITRLPLLNGGFSAAYLVGLGDRAKNGKESALHENIRSRSAELLRRC
                     RADGVSKITVVLPDAPSFGTSCALAEGAELGAYAFSKYKSIPEEERLPEPAELMLLDG
                     VDEGVSRGKMFACSQNWAKDIANEPGNKITPVSMADMAVQWGKDFGFEVSVWDEQRLL
                     QEGMEGIYSVGMGSAHPPRLIHMVYRPSDSVRKVALVGKGVTFDSGGLNVKTGDSMRT
                     MKGDKTGASNVFAILRGAAKMNIPCELHGIVPVAENMPGGKSFRPDDILRIRNGKTVE
                     IENTDAEGRLLLADALCFACEQKPDAIIDMATLTGACAVALGQNMAGLFTPDDAFADG
                     FLEVSARRGERFWRLPMDDDRIAEAMKSPVA"
BASE COUNT          338 a          351 c          472 g          330 t
ORIGIN      
        1 tgcggtgatt atctccgcgt tgttccttgg agagtctgtg agtgcatcga tgcttttcgg
       61 cggagcaatg gtattttccg gcgtctggtg cgtgaacagc ctgtcccgaa tgcaggaaaa
      121 acggcggaaa atggagtagc ggtctctacc gaataacgag tgaagaactg gagaatgtat
      181 tgaggtcgag gattcataag ctggaaagga gagttgcgtt caatgaaaat tcttacagcc
      241 ccctcaaacg ggggctttgt tcgtgaagga gctttggctc tgttcgtgtt ctccaaagaa
      301 tccgtatccg gtggtttgct tgatgagcac tttgcggata tggccgcacg gctgatggaa
      361 gagccctctt tcaggggaaa aaaagggaaa ataacgcgat tgccccttct gaacggggga
      421 ttttccgcgg cgtaccttgt cggtctcggg gaccgtgcaa agaacggcaa agagtctgct
      481 cttcatgaga atatccgttc gagaagcgcc gaacttctgc ggcggtgcag ggccgacgga
      541 gtttcgaaaa tcactgtcgt cctgccggat gcgccttcat tcgggacgag ctgcgctctt
      601 gcggaaggcg ctgaactggg agcgtacgct ttttctaagt acaagagtat tcccgaggag
      661 gaacgactac ctgaaccggc ggaactcatg ctcctcgacg gcgtcgacga aggcgtgagc
      721 cggggcaaga tgtttgcctg ctcacagaac tgggcgaaag atatcgcaaa tgaaccagga
      781 aacaaaatca ccccggtgtc aatggcggac atggctgttc aatgggggaa ggattttggt
      841 ttcgaggttt ccgtctggga tgagcaacgg cttctccaag aaggaatgga ggggatctat
      901 tccgttggaa tgggttcggc gcatccgcct cggttgattc acatggtgta caggccgtcg
      961 gattctgttc gaaaggtcgc gctcgtcggg aaaggcgtca cgttcgacag cggaggactg
     1021 aacgtaaaga ccggagattc tatgcggacg atgaaaggcg acaaaacggg agcgtcaaat
     1081 gtgtttgcta ttcttcgagg cgccgcgaag atgaacatcc cctgcgaact gcacgggatt
     1141 gtcccggtcg ctgaaaacat gcccggcggc aaatcgtttc gcccggacga tatactgcgt
     1201 atccggaacg gcaaaactgt ggagatagag aatacggacg cggagggacg gcttcttctc
     1261 gccgacgcgc tctgttttgc ctgcgaacag aagcccgatg cgatcatcga catggcgacg
     1321 ctcaccggag cgtgcgcggt agccttggga cagaacatgg cgggcctctt cactcctgac
     1381 gacgcgtttg ccgatgggtt tttggaggtt tctgcgagga gaggagagcg attctggcgt
     1441 cttcccatgg atgacgaccg gattgcggaa gcgatgaagt ctccggtagc c
//
LOCUS       DAOTIL010000771         1490 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminobacteriaceae bacterium isolate AMR_MDS_3026
            NODE_101445_length_1490_cov_2.827875, whole genome shotgun
            sequence.
ACCESSION   DAOTIL010000771 DAOTIL010000000
VERSION     DAOTIL010000771.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868050
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminobacteriaceae bacterium (wastewater metagenome)
  ORGANISM  Aminobacteriaceae bacterium
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae.
REFERENCE   1  (bases 1 to 1490)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1490)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:19:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,224
            CDSs (total)                      :: 3,198
            Genes (coding)                    :: 3,184
            CDSs (with protein)               :: 3,184
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1490
                     /organism="Aminobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_101445_length_1490_cov_2.827875"
                     /isolate="AMR_MDS_3026"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:3034609"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..309
                     /gene="fliN"
                     /locus_tag="P5201_13080"
     CDS             <1..309
                     /gene="fliN"
                     /locus_tag="P5201_13080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012870064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009288 - bacterial-type flagellum
                     [Evidence IEA];
                     GO_function: GO:0003774 - cytoskeletal motor activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor switch protein FliN"
                     /protein_id="HRV99524.1"
                     /translation="VRPAEFVPLTQKSVSGFGSKIDLVADIPVRVTVELGRTRKNIAD
                     ILNMTPGSVIELDKLAGEPVDVLVNSKLIAKGEVVVIDENFGVRITEIVTSAGRVHSL
                     "
     gene            361..723
                     /locus_tag="P5201_13085"
     CDS             361..723
                     /locus_tag="P5201_13085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016290481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HRV99525.1"
                     /translation="MGRKVLIVDDAAFMRMMLKDILTKNDFEVVGEAENGKVAVASYQ
                     KLQPDIVTMDITMPEMNGIDAVKAIKTLDASARVVMVSAMGQQPMVIEAIQAGAVDFI
                     VKPFQPDRVVEALNKALG"
     gene            741..1094
                     /locus_tag="P5201_13090"
     CDS             741..1094
                     /locus_tag="P5201_13090"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRV99526.1"
                     /translation="MVCRGAAGLLLFVPSAAFASSMEAPSLTSYLLKVGLSLALLAIA
                     GYLLVFFSGRRTPLCTKGALSVVASLPLGRDVVFVFRCGPDVIALLSGKNGARVIGRW
                     RYEEWQVDWNETSDS"
     gene            1075..>1490
                     /locus_tag="P5201_13095"
     CDS             1075..>1490
                     /locus_tag="P5201_13095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006583101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthetic protein FliP"
                     /protein_id="HRV99527.1"
                     /translation="MKLRIPKSVAFFFAPLMILLGSLAAYAQEGAQFIPIPVLQFGLG
                     VAESPRDVATTLQILALLTVLSVAPGIILMMTCFTRILIVLGFVQRAIGLQQTPPSQV
                     IVSLALFLTLFIMAPTWNKIYDNALSPYLAGTVNSA"
BASE COUNT          294 a          376 c          442 g          378 t
ORIGIN      
        1 gtgcggccgg ctgagttcgt tcccttgacg cagaaatcgg tctccggatt cggcagtaaa
       61 atcgaccttg tcgcggatat ccctgtccgt gttactgttg aattgggaag aactcgaaaa
      121 aatatcgccg acatattgaa catgacgccc ggttccgtga tagaattgga taaattggcc
      181 ggtgagccgg tcgatgttct cgtgaacagc aagctgatcg ccaaaggcga ggttgtggtt
      241 atcgacgaga atttcggcgt ccgcatcacg gagattgtga catccgccgg tcgagtccat
      301 tcgttgtagc gaaatggccg atgaactttt cagttattac cgagagaggg atgtggaagt
      361 atgggcagaa aagtactgat cgttgatgat gccgccttca tgaggatgat gctgaaggac
      421 attctgacca agaacgattt tgaagtcgtt ggcgaggcgg agaacggcaa ggtcgccgta
      481 gcgtcgtacc aaaagcttca gcctgatata gtcaccatgg atatcaccat gccggagatg
      541 aacggtattg atgccgtgaa agccatcaaa actcttgatg cttccgcgag ggtcgtgatg
      601 gtgagcgcca tggggcagca gccgatggtc atcgaggcta ttcaggcagg ggccgttgac
      661 ttcatcgtca agccctttca gccggacagg gtggtcgaag cgctgaacaa ggcgctcggt
      721 taggagcgcg ggcgctcttc atggtgtgcc ggggcgctgc gggtctgctt cttttcgttc
      781 cctccgcagc cttcgcttcg tcaatggaag ctccctctct gacgagctac ctgttgaagg
      841 taggactgag cctggcgctc cttgcgatcg ccggatactt gcttgtcttc ttttccggga
      901 ggagaacgcc tctctgcacg aaaggagctc tttccgtcgt agcatccttg cctctcggca
      961 gggatgttgt ttttgttttt cgttgcggtc ccgatgtgat agcgcttctt tcagggaaaa
     1021 acggggcgcg cgtgattggc aggtggcgct atgaagagtg gcaggtcgat tggaatgaaa
     1081 cttcggattc ctaagtccgt cgcgttcttt ttcgcgccgt tgatgatctt gcttggttcg
     1141 ctcgccgcat acgcccagga gggagcgcaa tttattccca taccggtgct ccagttcgga
     1201 ctcggagtag cggagtctcc ccgcgacgtg gcgacgaccc tgcagatcct cgcgcttctg
     1261 accgtgctga gcgtggcgcc cgggattatc cttatgatga cctgttttac gcgtattttg
     1321 attgtcctcg gattcgtcca gcgggctatc ggcttgcagc agacgccgcc gagccaggtg
     1381 atcgtcagtc tcgcgctttt tctgacgctg ttcatcatgg cgccgacgtg gaataaaatt
     1441 tacgacaatg ctctctctcc gtatcttgca ggaacggtta actcagccca
//
LOCUS       DAOTIL010000772         1488 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminobacteriaceae bacterium isolate AMR_MDS_3026
            NODE_101654_length_1488_cov_2.957432, whole genome shotgun
            sequence.
ACCESSION   DAOTIL010000772 DAOTIL010000000
VERSION     DAOTIL010000772.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868050
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminobacteriaceae bacterium (wastewater metagenome)
  ORGANISM  Aminobacteriaceae bacterium
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae.
REFERENCE   1  (bases 1 to 1488)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1488)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:19:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,224
            CDSs (total)                      :: 3,198
            Genes (coding)                    :: 3,184
            CDSs (with protein)               :: 3,184
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1488
                     /organism="Aminobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_101654_length_1488_cov_2.957432"
                     /isolate="AMR_MDS_3026"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:3034609"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..185
                     /locus_tag="P5201_13100"
     CDS             <1..185
                     /locus_tag="P5201_13100"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRV99528.1"
                     /translation="DDAVGAFGERVKRAAFGFPPTQDENMERTRCTEVNTTLFVLKNA
                     VDFFEKGLMFPVLSRA"
     gene            730..>1488
                     /locus_tag="P5201_13105"
     CDS             730..>1488
                     /locus_tag="P5201_13105"
                     /inference="COORDINATES: protein motif:HMM:NF014763.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cache domain-containing protein"
                     /protein_id="HRV99529.1"
                     /translation="MNTLRARLIATFLIVAVVAVGVVGLISVTRARQAIIDAAWKEGD
                     ALVSTLSDHIDSLLAERATVLELMAGQQSVRSMEYAAQQPVLMPLYDQYNFSDIFVVN
                     LEGDTTDVKLGPQKVNIKDRDYFIKAVKETKTVISQPAVNRRTGMLTYFYASPIVKDG
                     TVVGVLVAGENIENVAKAASSTTWGTSGYAYVTDSKGVLIAHPQKDFIGTLNPSVESD
                     RVAPELARGMRDGLAGNKGRIEYFFNGRDQMNAYA"
BASE COUNT          363 a          356 c          424 g          345 t
ORIGIN      
        1 tggacgatgc ggtcggcgct ttcggcgaga gagtgaaaag agcggcgttc ggctttcccc
       61 cgactcagga tgagaacatg gaaaggacgc gctgtacgga ggtgaatacg accctcttcg
      121 tcctgaagaa cgcggtggat ttttttgaga aagggctgat gttccccgtc ctctcccgtg
      181 cctgagggaa ccgtcttctt tttctcgttg cccgacaggc tgaacggcga tcctgcggaa
      241 gagcgcggag tgtgacggaa cgaaaggagg tcgccgatga gcgtttttga taacgacgca
      301 tcaacgtaca accaacggta tacgactccc atggggcggc atgtcgatga tgtggagaca
      361 agctgtgctc tcgccctttt acagccgtta gtcgttttct cgctagggaa tgaggagttc
      421 ggcatcgata tctcgcgggg gcgcggaaga tcgttcccat cgcggccgct tcagtacaag
      481 aggtttgata tcggcagata aacgtcagct ttacaataat aattctataa tgcagacttt
      541 taaagaaaaa aactgttgaa ccgataaata gattacatca agatgcaccc tacaacgaat
      601 aaaatccatt catttactcg gaatatataa ataagtgggc atcttctggg tcgtgcaggg
      661 gttggggagg ttgtgtgtaa caaggacgtt ttcatttccg tccgaattcc aaaaaggggg
      721 tacgttttta tgaacacgct tcgagcacgt cttattgcaa catttcttat cgtcgccgtg
      781 gttgcggtgg gggtcgtcgg tctgatttcg gtgacccgcg cccgccaggc aattatcgac
      841 gcagcctgga aggagggcga cgcgctggtt tccacgctct ctgatcatat cgacagtctt
      901 cttgcggaaa gagcaacagt ccttgagctc atggccggcc agcagagcgt tcgctccatg
      961 gagtacgcgg cgcagcagcc agtgctgatg ccgttgtacg atcagtataa tttttcggat
     1021 atcttcgtgg tcaacctcga aggggacacc acggatgtca agctggggcc tcaaaaggtg
     1081 aatatcaagg acagagacta tttcataaag gctgtcaaag agacaaaaac cgtgatctct
     1141 cagcctgccg tcaacagacg aaccggtatg ctgacatatt tctacgcatc gcctatcgta
     1201 aaagatggaa ccgtggtcgg cgtgcttgtt gcgggggaga atatagagaa tgtcgccaag
     1261 gcagcctcgt cgactacatg gggtacttcg ggatatgcgt atgttaccga cagcaaaggc
     1321 gtgctcatcg cgcatcctca aaaggatttt atcggaacct tgaatccgtc ggtcgagagc
     1381 gaccgcgtcg cccctgaact tgcacgcgga atgcgcgacg gcctggccgg gaacaaggga
     1441 aggatagagt acttcttcaa cggcagggat cagatgaacg cctacgct
//
LOCUS       DAOTIL010000773         1485 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminobacteriaceae bacterium isolate AMR_MDS_3026
            NODE_101910_length_1485_cov_3.846154, whole genome shotgun
            sequence.
ACCESSION   DAOTIL010000773 DAOTIL010000000
VERSION     DAOTIL010000773.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868050
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminobacteriaceae bacterium (wastewater metagenome)
  ORGANISM  Aminobacteriaceae bacterium
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae.
REFERENCE   1  (bases 1 to 1485)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1485)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:19:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,224
            CDSs (total)                      :: 3,198
            Genes (coding)                    :: 3,184
            CDSs (with protein)               :: 3,184
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1485
                     /organism="Aminobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_101910_length_1485_cov_3.846154"
                     /isolate="AMR_MDS_3026"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:3034609"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            42..527
                     /locus_tag="P5201_13110"
     CDS             42..527
                     /locus_tag="P5201_13110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006301569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HRV99530.1"
                     /translation="MEQDVPLILSFIRELAVYEKMLDDVVATEDGLKEHLFGERRVAE
                     VLIAEWEGAPVAFALFFHNFSTFLGRPGIYLEDLYVKPEMRGKGIGKAMLSHIANTAK
                     KRGCGRFEWSVLDWNEPAITFYRSIGAVAMDEWEHFRLSGEALETVAEMCAPENVYPK
                     Q"
     gene            complement(602..>1485)
                     /locus_tag="P5201_13115"
     CDS             complement(602..>1485)
                     /locus_tag="P5201_13115"
                     /inference="COORDINATES: protein motif:HMM:NF019581.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HRV99531.1"
                     /translation="VAENLGIPGQVLHHKACGRRNRGRVRETMRLLEEARERGVDMTA
                     DQYPYAAGSTTLRSILPPWIHEGGVEKLIERLSGPALRERLKREIPEAEGWENMLRNS
                     GGPEGVMMLYCPKTPDFEGKRLTEIGAMTGKDPLDAAFDIIIENRGSDTACFFQLAEE
                     DVQFVMKHPLVMVASDSIPPAPGAKCHPRTNGTFPRVLGKYVREEKTLRLEEAVWKMS
                     GFPAARLGLSKKGLIREGMDADLVLFDPETVVDGATFEDPFGEPKGISHVFVAGRLAV
                     KNGEYTGAAAGKVLRKV"
BASE COUNT          279 a          482 c          427 g          297 t
ORIGIN      
        1 actacacaac cgctctaccc ggcttgatca tccgggatgc cgtggaacag gatgttccgc
       61 tcatcctctc gttcatccgg gaacttgccg tttatgaaaa aatgctcgac gacgtcgtcg
      121 ccacagagga cggactgaaa gagcacctgt tcggcgaacg ccgcgtcgca gaagttctca
      181 tagccgagtg ggagggcgct cccgtcgcct tcgcgctctt cttccacaac ttctcaacct
      241 tccttggacg tccgggaatc tacctggagg acctctatgt gaagccggag atgcgcggga
      301 aggggatcgg gaaggcgatg ctctcccaca tcgcgaacac cgcaaagaaa cgcgggtgcg
      361 gccgtttcga gtggtcggtg ctcgactgga acgaaccggc gatcacattc taccgctcca
      421 tcggagcagt ggcgatggac gagtgggagc acttccgcct gagcggggag gctctggaaa
      481 cggtcgccga aatgtgtgca ccggagaacg tgtatcccaa acagtgaaga aaacgctcac
      541 tcgatacgac aaagagcggg gtcccatccg agggaacccc gctctttgtt gcatcctacg
      601 cttacacctt ccgaagcacc ttccccgccg cggcgccggt gtactcgccg ttcttcacgg
      661 cgagacgccc cgccacaaag acgtgggaga tgcccttcgg ctcgccgaag gggtcctcga
      721 aggtcgctcc gtcgacgacg gtctccgggt cgaagaggac gagatcggcg tccattcctt
      781 ctctgatgag tccttttttt gacagcccga gccgtgctgc cgggaagccg ctcatcttcc
      841 aaacggcctc ttcgagacga agggtctttt cctcccggac gtatttgccg agaacgcggg
      901 gaaacgtccc gttcgtacgt gggtggcact tcgctccggg cgccggagga atactgtcgg
      961 aagccaccat gacgagaggg tgcttcatga cgaactgcac gtcttcctcc gcaagctgaa
     1021 agaagcatgc cgtgtcggaa cccctgtttt caataatgat atcgaatgcc gcgtcgagcg
     1081 gatctttccc cgtcatcgca ccgatctccg tcaggcgttt tccctcgaaa tccggcgttt
     1141 tcgggcagta gagcatcatc acaccctccg gaccgccgct gttgcgcagc atgttctccc
     1201 acccttcggc ctccgggatc tcccgtttca gacgctcgcg aagcgcgggg ccggagagac
     1261 gttcgatcag cttctccacc cctccctcgt gaatccaggg gggaaggatg ctccggagcg
     1321 tggtgctccc cgccgcatac ggatactgat ccgccgtcat gtcgacgccg cgctccctgg
     1381 cctcctcaag cagtctcatg gtctcacgga ccctgccccg gttcctcctg ccgcaggcct
     1441 tgtggtggag cacctgcccc ggaatcccga ggttttccgc cacgg
//
LOCUS       DAOTIL010000774         1483 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminobacteriaceae bacterium isolate AMR_MDS_3026
            NODE_102086_length_1483_cov_3.800420, whole genome shotgun
            sequence.
ACCESSION   DAOTIL010000774 DAOTIL010000000
VERSION     DAOTIL010000774.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868050
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminobacteriaceae bacterium (wastewater metagenome)
  ORGANISM  Aminobacteriaceae bacterium
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae.
REFERENCE   1  (bases 1 to 1483)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1483)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:19:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,224
            CDSs (total)                      :: 3,198
            Genes (coding)                    :: 3,184
            CDSs (with protein)               :: 3,184
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1483
                     /organism="Aminobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_102086_length_1483_cov_3.800420"
                     /isolate="AMR_MDS_3026"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:3034609"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(84..914)
                     /locus_tag="P5201_13120"
     CDS             complement(84..914)
                     /locus_tag="P5201_13120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013047461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HRV99532.1"
                     /translation="MSNNGNDSASSSFIPALEVRDLRKSFGNVSVLEGISLSVREGEV
                     ISVIGPSGSGKSTLARCICRLEEIDGGEVLLYGDRIDNGKHSNRDIARLVGMIFQQFN
                     LFPHLSVLENIILSPVRILGNSPKAAEDKAMSLLERVGLCEKKSARPNELSGGQRQRV
                     AIARALAMNPKIMLFDEPTSALDPELVGEVLDVIADLAQSGMTMMIITHEMLFAKEVS
                     DRVLFMDGGKILEQGPPDSIFTSPQVPRTQLFLQRMLAHVGKELVQAHSDERTWTHDA
                     "
     gene            complement(1032..1160)
                     /locus_tag="P5201_13125"
     CDS             complement(1032..1160)
                     /locus_tag="P5201_13125"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRV99533.1"
                     /translation="METVRKENQGEKVFAEKGEKDDRFDEVMADMNQAAYRALLGQ"
BASE COUNT          391 a          377 c          358 g          357 t
ORIGIN      
        1 agagcagcga aagaaaagaa gcctggatgg tgataacggc tccgcgaagc agcatatgaa
       61 gatacggaac gatggcggaa aaatcaagcg tcatgagtcc acgtcctctc atcggaatgc
      121 gcttgtacga gttctttgcc gacatgagcg agcattcgct gaagaaaaag ctgtgttctc
      181 ggaacttgcg gggatgtaaa aatcgagtcc ggaggtccct gttcgagaat tttccccccg
      241 tccataaaga gcacccggtc agacacttct tttgcaaaga gcatctcatg tgtaatgatc
      301 atcatggtca ttccggactg tgctagatcg gcgataacgt caagcacctc ccccacaagt
      361 tccgggtcga gggcgctggt tggctcatcg aagagcataa tcttgggatt cattgcgagg
      421 gcgcgggcga ttgctacacg ttgacgttgt cctccggaga gctcgttggg acgcgctgac
      481 tttttctcgc agagtcctac acgctccaga agcgacatcg ctttatcttc cgcagccttc
      541 ggagagtttc ccaaaatgcg gacagggctc aaaatgatat tctcaagaac tgacagatgg
      601 gggaaaagat tgaactgctg aaaaatcatc cctacgaggc gggcaatatc acgattcgaa
      661 tgcttgccgt tgtcaatcct gtctccgtag agcaaaacct cccccccgtc gatttcctca
      721 agacggcaga tacaccgtgc aagcgtactc tttcctgaac cgctcggacc tatcaccgag
      781 atgacttctc cttcacggac ggaaagcgag atgccctcaa gaacagatac attgccgaat
      841 gatttccgaa gatcccgcac ctcaagagcg ggtataaacg atgatgacgc actatcgttg
      901 ccgttgttgg acacacctct cacctcccga aagctgccgt tattatactc taccggagga
      961 cgagtcggca aaaaacagaa aagccggcga atgccggcgc gcagatattc tcccgaaacg
     1021 aagagcgtgt tctactgccc cagaagagcc ctgtaggccg cctggttcat atcggccatc
     1081 acttcatcga accggtcatc tttttcgcct ttctccgcaa acactttttc cccttgattt
     1141 tccttccgaa cagtttccat gatttttcct cccttacagt gcaacacagt ctgttgtgta
     1201 taatatattt atggtccgta ccatagttgt tttttattat agcgcttttt tcgcacgacg
     1261 caagaggctt gcaaacattt cttcttcacg agagaataaa gaaaacacga aaggaagtga
     1321 tcaagtggag aaacgccacc tcaaaggctc gacgtttttt ttccctggca aagtgaatgt
     1381 gggctatttc cagaaaaacg aagatgtctg gcttgtggat accggcttgg atgatgaagc
     1441 ggggcggaag atcgcacgtt ttttagagac agaaaataaa aaa
//
LOCUS       DAOTIL010000775         1482 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminobacteriaceae bacterium isolate AMR_MDS_3026
            NODE_102161_length_1482_cov_4.126840, whole genome shotgun
            sequence.
ACCESSION   DAOTIL010000775 DAOTIL010000000
VERSION     DAOTIL010000775.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868050
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminobacteriaceae bacterium (wastewater metagenome)
  ORGANISM  Aminobacteriaceae bacterium
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae.
REFERENCE   1  (bases 1 to 1482)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1482)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:19:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,224
            CDSs (total)                      :: 3,198
            Genes (coding)                    :: 3,184
            CDSs (with protein)               :: 3,184
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1482
                     /organism="Aminobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_102161_length_1482_cov_4.126840"
                     /isolate="AMR_MDS_3026"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:3034609"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..916
                     /locus_tag="P5201_13130"
     CDS             <1..916
                     /locus_tag="P5201_13130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009202426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HRV99534.1"
                     /translation="KDHYDEKWIIENVRKACRLAQKYGNTHIRAFADTDTKAKLEGIK
                     ALLKAREEFKDTVDLQVVAFPQDGVVRDPGADGYIRSALELGADVVGGIPWIEYTDAD
                     MQAHIDSMFALAKEFGKDVSMLIDDAGDSGLRSLEMLAVKTIKEGWQGRVTAQHCRAM
                     AMYQEPYFRKVLALLQKAKIGLVSDPHTGPLHARVRDLYNAGVAVALGQDDIADAYYP
                     FGRNNMLEIAFLAVHLLWMTTFSDMEILYDLITTNAAKAMGIKGHVLEPGGAADLVVL
                     NAEDVYHAIWEHEAPLHVIRKGKDVTLK"
     gene            complement(1063..>1482)
                     /locus_tag="P5201_13135"
     CDS             complement(1063..>1482)
                     /locus_tag="P5201_13135"
                     /inference="COORDINATES: protein motif:HMM:NF014691.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fic family protein"
                     /protein_id="HRV99535.1"
                     /translation="VTEPGRIPSEMASLFEWANSERGGYHPVEFAAQLHKRFVFIHPF
                     IDGNGRISRLLMNAALIQDGYLPALIPPSLRREYIDLLERARTDDRPFAEFIAARVLE
                     SEKEMLRLLHIPEPEGRYDLDGLVSGITKENGHGELD"
BASE COUNT          296 a          491 c          459 g          236 t
ORIGIN      
        1 gaaggaccac tacgacgaga agtggatcat cgagaacgtc cgcaaggcgt gccgtctggc
       61 ccagaaatac ggcaacaccc acatccgcgc cttcgccgac acggacacca aggcgaagct
      121 ggaggggatc aaggcgctgc tgaaagcccg cgaggagttc aaggacaccg tggacctcca
      181 ggtggtcgcc ttcccgcagg acggcgtggt gcgcgacccg ggcgcggacg ggtacatccg
      241 ctcggcgctc gagctggggg ccgacgtcgt gggcggcatt ccgtggatcg agtacaccga
      301 cgccgacatg caggcgcaca tcgactcgat gttcgccctc gccaaggagt tcggcaagga
      361 cgtctccatg ctgatcgacg acgcgggcga ctccgggctc cgctcgctcg aaatgctcgc
      421 cgtcaagacg atcaaggagg ggtggcaggg gcgcgtgacc gcgcagcact gccgcgccat
      481 ggccatgtac caggagccct acttccgcaa ggtcctcgcc ctgctgcaga aagcgaagat
      541 aggcctcgtc agcgacccgc acaccggccc gctccacgcg cgcgtgcgcg acctgtacaa
      601 cgcgggcgtg gccgtcgcgc tggggcagga cgacatcgcc gacgcctact accccttcgg
      661 ccggaacaac atgctggaga tagccttcct cgccgtccac ctgctctgga tgaccacgtt
      721 cagcgacatg gagatcctct acgacctgat caccacgaac gcggcgaagg ccatggggat
      781 caaggggcac gtcctcgagc ccggcggcgc cgccgatctc gtcgtactca acgcggagga
      841 cgtctaccac gccatctggg agcacgaggc gcccctccac gtcatccgca agggcaagga
      901 cgtcacgctg aagtaggcgc cgcaagagat cgcaagaaag cacgccgccc ccggagaagc
      961 tttccggggg cggcgcgttg tcgatcatat caatgtatat caaggaacat ccaaaagggc
     1021 atccgggatg caaaccggct gctccttacc ggccttctcg cctcagtcca actcgccgtg
     1081 accgttttcc ttcgttattc ccgagacaag cccgtccaag tcgtagcgac cctccggttc
     1141 cggtatgtgc agcaggcgca gcatctcttt ctcgctctcc agaacccgcg cggcgatgaa
     1201 ctccgcgaac gggcggtcgt cggttcgcgc acgctccagc agatcgatat actccctgcg
     1261 cagactcgga ggtataaggg ccggtagata gccgtcctgt atcagcgcgg cgttcatcag
     1321 caaccgggag atgcggccgt ttccgtcgat gaacggatgg atgaagacga aacgcttgtg
     1381 gagctgcgcg gcgaactcga ccggatgata tccgccgcgt tcgctgttcg cccattcgaa
     1441 gagagatgcc atctccgagg gaattcggcc cggttccgtc ac
//
LOCUS       DAOTIL010000776         1481 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminobacteriaceae bacterium isolate AMR_MDS_3026
            NODE_102296_length_1481_cov_2.903226, whole genome shotgun
            sequence.
ACCESSION   DAOTIL010000776 DAOTIL010000000
VERSION     DAOTIL010000776.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868050
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminobacteriaceae bacterium (wastewater metagenome)
  ORGANISM  Aminobacteriaceae bacterium
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae.
REFERENCE   1  (bases 1 to 1481)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1481)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:19:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,224
            CDSs (total)                      :: 3,198
            Genes (coding)                    :: 3,184
            CDSs (with protein)               :: 3,184
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1481
                     /organism="Aminobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_102296_length_1481_cov_2.903226"
                     /isolate="AMR_MDS_3026"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:3034609"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1080
                     /locus_tag="P5201_13140"
     CDS             <1..1080
                     /locus_tag="P5201_13140"
                     /inference="COORDINATES: protein motif:HMM:NF024754.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' exonuclease"
                     /protein_id="HRV99536.1"
                     /translation="RPNSMNFTPNVAVSRDCFSSLFKMPRARQEKVLEFIRRFSERPD
                     SPGINFEKIRDAKDKKLRSVRIDDAYRGIVLHPESGNTYVLLWVDHHDDAYAWARNRS
                     CAVNPYTGSLQIFEVCEEKRAAEPAVSETPPLFAGTGDPHQRIYRHPVVLSRCGISVA
                     GRSCKLLVNYRTTEETRRWAVSLLDGRSLDDLEGGEDDLKGYRSLLRGQEPRLEGFGS
                     FDEEADFLVAFLKEIESKGERLKDVCLVVRSRALLERYREALAGRGLPLVKLEAGGPE
                     NRDEDGVRTATMHRVKGLEFDRMILAGVCEDIVPPKSWREHEDPPMMNELETRERSLI
                     HVAATRARTHILVTWHGEPSPLLGL"
     gene            complement(1275..>1481)
                     /locus_tag="P5201_13145"
     CDS             complement(1275..>1481)
                     /locus_tag="P5201_13145"
                     /inference="COORDINATES: protein motif:HMM:NF026183.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-binding protein"
                     /protein_id="HRV99537.1"
                     /translation="TPGGKMWLHVKDLKNVMTACREYDVAMPLTESVLAMMEDVVRDA
                     GPDTDHGGVAKYFEKLNGVSLATK"
BASE COUNT          270 a          443 c          494 g          274 t
ORIGIN      
        1 aggccgaaca gtatgaactt cacgccgaac gtcgcagtct ccagggactg cttctcctcg
       61 ctcttcaaga tgccgcgcgc ccggcaggag aaggtgctgg aattcatcag gcgcttcagc
      121 gagcggccgg actctccggg gatcaatttc gagaagatcc gcgacgcgaa ggataagaag
      181 ctgcgctccg tgcggatcga cgacgcctac cgcggcatcg tgctgcaccc ggagtcggga
      241 aacacctacg tactcctctg ggtcgaccat cacgacgacg cctacgcgtg ggccagaaac
      301 cgctcgtgcg cggtgaaccc ctacacgggg agcctgcaaa tcttcgaggt ttgcgaggag
      361 aagcgagcag cggaaccggc cgtctcggag acgccgccgc tcttcgcagg gacgggcgac
      421 ccgcaccaga ggatataccg ccaccctgtc gtgctctcgc gctgcggcat ttccgtcgcc
      481 gggcggagct gcaagctgct ggtgaactac cgcaccaccg aggagaccag gcgctgggcg
      541 gtctccctgc tggacgggcg ttctctcgac gatcttgagg gaggggagga cgacctgaag
      601 gggtaccgtt cgctgctgcg cggtcaggag ccacgtctgg aggggttcgg atcgttcgac
      661 gaggaggcgg attttctggt cgcgttcctg aaggagatcg agtccaaggg ggagcgattg
      721 aaggacgtct gcctggtcgt ccgctcgcgg gcgcttctgg aacgttaccg cgaggcgctt
      781 gccgggcgag gtcttcctct ggtgaagctg gaggccgggg ggcccgaaaa ccgcgacgag
      841 gatggcgtcc ggacggcgac gatgcaccgg gtgaaggggc ttgagttcga ccggatgatc
      901 ctcgcaggcg tctgcgagga catcgtcccg ccgaagtcgt ggcgggagca tgaggatccc
      961 ccgatgatga acgagctgga aacccgcgag cgctccctga tccatgtcgc cgcgacgagg
     1021 gcgcggacgc acatcctggt gacctggcac ggcgaaccct ccccgctgct ggggctgtaa
     1081 agcggaagag cctgccgacg ctgagaatca gggactgtac tgtttccagc atttagcatc
     1141 acccggccgt aatttttacc gatatttgcc taaggtggta ataaatatta cctgtttcag
     1201 catggaggtt aaagggaacg agcggtgtgc gttgactcaa gccttcccgg ttttgccggt
     1261 gccagcgtca cgccctattt cgtcgccagc gagacgccgt tgagcttctc gaagtacttg
     1321 gcgacgccgc cgtggtcggt gtcggggccc gcgtcccgca cgacatcctc catcatcgcc
     1381 agaacgcttt cggtcagcgg catggcgacg tcgtactcgc ggcaggcggt cattacgttc
     1441 ttcaagtcct tgacgtggag ccacattttt ccgccggggg t
//
LOCUS       DAOTIL010000777         1480 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminobacteriaceae bacterium isolate AMR_MDS_3026
            NODE_102359_length_1480_cov_5.687719, whole genome shotgun
            sequence.
ACCESSION   DAOTIL010000777 DAOTIL010000000
VERSION     DAOTIL010000777.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868050
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminobacteriaceae bacterium (wastewater metagenome)
  ORGANISM  Aminobacteriaceae bacterium
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae.
REFERENCE   1  (bases 1 to 1480)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1480)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:19:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,224
            CDSs (total)                      :: 3,198
            Genes (coding)                    :: 3,184
            CDSs (with protein)               :: 3,184
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1480
                     /organism="Aminobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_102359_length_1480_cov_5.687719"
                     /isolate="AMR_MDS_3026"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:3034609"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..871)
                     /locus_tag="P5201_13150"
     CDS             complement(<1..871)
                     /locus_tag="P5201_13150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005661031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal-phosphate dependent enzyme"
                     /protein_id="HRV99538.1"
                     /translation="MLHCFQCGKTFDEASLLWRCPCGGVLDWTVPEPFKRSSIRTEET
                     GMWRYAASLPLDSPDSRVSMGEGGTPLIPGDWNGLSVFWKLDFLCPTGSYKDRGMACL
                     VNRLRELGVRELIEDSSGNAGSSMAAYCALAGIGCKIYVPGYTSEGKCVQILSYGGEL
                     VRIPGTREDTSRAAEKAASETYYASHNWSPYFAHGVKTFAFEVWEQLGFQLPDAVLLP
                     AGQGSLVLGWAQAFEELRASGEIDRLPRIYALQPENCAPLALAFEKRTDAPEPFPKKE
                     TIAEGISSAEPVRG"
     gene            1024..>1480
                     /locus_tag="P5201_13155"
     CDS             1024..>1480
                     /locus_tag="P5201_13155"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013047774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metalloprotease"
                     /protein_id="HRV99539.1"
                     /translation="MKRVIGRLALLIVLLLAFSAVSEGALDRATVDRAWTRIAAHAGI
                     PWQQVIYEEQQEPNAWVKFSPGNHTVHVTTGLMAILTKEDEIAGILAHEAGHIQLGHY
                     SSSVSRNLLWGLLFNSLKGDAAREIVGGVGMVLAESGFSREQEVEADDYG"
BASE COUNT          363 a          400 c          395 g          322 t
ORIGIN      
        1 ccccgcgaac cggttccgca gaactgatac cttccgctat ggtctctttc tttgggaatg
       61 gctcgggagc gtcggtccgc ttttcgaagg cgagcgccag aggtgcgcag ttttcgggct
      121 gaagcgcgta gattctcgga agacggtcta tctctccaga ggcgcgcagt tcttcgaatg
      181 cttgcgccca tccaagaaca agacttccct gaccggcagg aagaagaacc gcgtccggaa
      241 gctggaatcc caactgctcc catacttcga aggcaaaggt ttttacgccg tgggcgaagt
      301 aggggctcca gttatggctg gcataatagg tttcactcgc cgctttctcc gccgcacggg
      361 aagtatcctc tctggtaccg ggtatacgga caagttcccc gccgtaagag agtatctgaa
      421 cgcacttgcc ttcggacgtg taccctggaa cgtagatttt acacccgatc ccggcaagag
      481 cgcagtatgc ggccatcgac gagcccgcat ttcctgagga gtcctctatg agctcacgca
      541 cgccgagttc gcgtaaacga ttgacaagac acgccattcc gcggtctttg tatgagcctg
      601 tagggcagag aaaatcaagc ttccagaaaa cggaaagccc gttccagtct ccaggaataa
      661 gaggagtccc tccctccccc atagaaacac gcgaatcggg agaatcaaga ggcagagaag
      721 cagcgtaccg ccacatcccg gtctcttccg tgcgtatcga cgagcgtttg aagggctcgg
      781 gtacagtcca gtcgagaaca cctccgcatg gacagcgcca caataaggat gcttcgtcga
      841 acgttttacc gcattgaaaa cagtgcagca tgcaatcaac ctcctctcga ttctgatttc
      901 attatataca gaatgtccga tactgcctat tgactcctac gaagtttccc cctttcacta
      961 aaagaagaaa ggatgtatcc tattgaaaat agaactatcc taaagaaaaa aggaggaatt
     1021 cgaatgaaga gggtaatcgg acgcctcgct cttcttatcg tacttcttct ggcgttttca
     1081 gctgtttccg agggcgctct cgacagagcg acggtagacc gggcgtggac gcgtattgcc
     1141 gctcatgccg gtatcccatg gcagcaggtc atttatgaag agcagcaaga accgaacgcc
     1201 tgggttaaat tttcgcccgg caatcacact gttcatgtga cgaccggcct gatggcgata
     1261 ctgaccaaag aagacgaaat agcgggcata cttgctcatg aagcgggaca catccagctc
     1321 ggccactatt caagctccgt aagccgcaat cttctctggg ggcttctgtt caattccctg
     1381 aaaggggatg ccgcgaggga gattgtcggc ggggtcggaa tggttcttgc cgaatccgga
     1441 ttcagcaggg aacaagaggt tgaagcagac gactacggta
//
LOCUS       DAOTIL010000778         1479 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminobacteriaceae bacterium isolate AMR_MDS_3026
            NODE_102483_length_1479_cov_3.287219, whole genome shotgun
            sequence.
ACCESSION   DAOTIL010000778 DAOTIL010000000
VERSION     DAOTIL010000778.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868050
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminobacteriaceae bacterium (wastewater metagenome)
  ORGANISM  Aminobacteriaceae bacterium
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae.
REFERENCE   1  (bases 1 to 1479)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1479)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:19:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,224
            CDSs (total)                      :: 3,198
            Genes (coding)                    :: 3,184
            CDSs (with protein)               :: 3,184
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1479
                     /organism="Aminobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_102483_length_1479_cov_3.287219"
                     /isolate="AMR_MDS_3026"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:3034609"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..220)
                     /locus_tag="P5201_13160"
     CDS             complement(<1..220)
                     /locus_tag="P5201_13160"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRV99540.1"
                     /translation="MSIKQRIGVFLALIILLAVPGLQAIAASAQETDVLALLEERQRA
                     VAELMEASTVYVLLDKGDSYSMGSAFIVG"
     gene            complement(352..1458)
                     /locus_tag="P5201_13165"
     CDS             complement(352..1458)
                     /locus_tag="P5201_13165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009163891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-TA family PLP-dependent enzyme"
                     /protein_id="HRV99541.1"
                     /translation="MRTHDLDTPALMIDREIMMKNLRDMQAYADRHGVALRPHTKTHK
                     APAIARMQVEGGAKGIAVAKVGEAETMAANGLNDIFIANEIVGVRKLERIRALSERID
                     ISFGVDSPCQAEAAEAVFAQADRPAQLLVEIEVGENRSGIIEEEDFVRLLQTIRSCPH
                     VEFKGLFSHDGNSYAASSQEECRRISVGAQRRTLRFAAIAREMGMPPRTVSIGSTPSL
                     MNESEIDRGVTEIRPGTYILMDASQGNAVGTLERCAATVLATVISRPTAERVILDVGA
                     KGLTMQGRSEGICATPGKGTLPDFPGVYIHAVYDEHAIIYNREFRETVRIGDKVRIIP
                     VHICPVCNLYDSAFLISGDEVVGEIPILCRGKLQ"
BASE COUNT          251 a          511 c          427 g          290 t
ORIGIN      
        1 cccccacgat gaaggcgctt cccatgctgt aggagtcccc cttgtcgagc agaacgtaga
       61 cggtggacgc ctccatgagc tccgccacgg cccgctgccg ttcctcgagg agagcgagca
      121 cgtccgtctc ctgcgcggag gcggcgatcg cctgaagccc cggtacggcg agaagaatga
      181 taagagcaag aaacactccg atacgttgtt tgatcgacac agtgcactcc accttcttaa
      241 gaatgttgtt tttcgataac tctttctttt ccgatagtac cttgccgaac ttccgatgtc
      301 aacaaaaggg ggcgaacgac gccgacgggc cgaaagccgg tgcggcaggc gttactgcaa
      361 ctttcccctg cacagaatcg gaatttcccc caccacttca tcgcctgaga tcaggaacgc
      421 gctgtcgtac aggttgcata ccggacagat atgcacgggg atgattcgca ccttgtcgcc
      481 gatccggacg gtctcgcgga actccctgtt gtagatgatc gcgtgttcgt cgtagaccgc
      541 atggatatac acgcccggaa aatccgggag cgtgcccttt cccggcgtcg cgcagatccc
      601 ctcgctccgc ccctgcatcg tcagcccttt ggcccccacg tccaggatca cccgctccgc
      661 tgtagggcgg ctgatgacag tcgccagcac cgtcgccgcg cagcgctcca gcgtgccgac
      721 ggcgttcccc tgcgacgcgt ccatcaggat gtacgttccc ggacggatct ccgtaacgcc
      781 cctatcgatc tccgattcgt tcatcagcga aggggtggag ccgatgctca ccgtccgcgg
      841 cggcatcccc atctcccgcg cgatcgccgc gaagcggagg gtgcgccgct gcgccccgac
      901 ggagattcgc ctgcactcct cctgcgagga ggccgcgtag ctgttgccgt cgtgggagaa
      961 gagccccttg aactccacgt gcggacagga gcggatcgtc tgcagcaggc ggacgaagtc
     1021 ctcctcctcg atgatgccgg agcggttctc gcccacctcg atctccacca ggagctgcgc
     1081 gggacggtcg gcctgcgcga agacggcctc cgccgcctcc gcctggcacg gggagtccac
     1141 gccgaaggag atgtcgatcc gctccgacag cgcgcgaatc cgctccagct tgcggacgcc
     1201 gacgatctcg ttggctatga agatatcgtt caggccgttc gcggccatcg tctccgcctc
     1261 gccgaccttt gccaccgcga tgcctttcgc accgccctcc acctgcatcc gcgcgatcgc
     1321 gggcgccttg tgcgtcttcg tgtgcgggcg cagcgcgact ccgtgccggt cggcgtacgc
     1381 ctgcatgtcc cgcaggtttt tcatcataat ttccctgtcg atcatcagcg cgggcgtgtc
     1441 cagatcatgc gttctcatgc atcgtcactc tccatctgt
//
LOCUS       DAOTIL010000779         1478 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminobacteriaceae bacterium isolate AMR_MDS_3026
            NODE_102565_length_1478_cov_4.290232, whole genome shotgun
            sequence.
ACCESSION   DAOTIL010000779 DAOTIL010000000
VERSION     DAOTIL010000779.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868050
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminobacteriaceae bacterium (wastewater metagenome)
  ORGANISM  Aminobacteriaceae bacterium
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae.
REFERENCE   1  (bases 1 to 1478)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1478)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:19:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,224
            CDSs (total)                      :: 3,198
            Genes (coding)                    :: 3,184
            CDSs (with protein)               :: 3,184
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1478
                     /organism="Aminobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_102565_length_1478_cov_4.290232"
                     /isolate="AMR_MDS_3026"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:3034609"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..669)
                     /locus_tag="P5201_13170"
     CDS             complement(<1..669)
                     /locus_tag="P5201_13170"
                     /inference="COORDINATES: protein motif:HMM:NF013088.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter"
                     /protein_id="HRV99542.1"
                     /translation="MSARTTSPLLYFSAYAALYIIWGSTYLAIRFTVETIPPFLSGSL
                     RFFLAGGILLVWCLYREQERPTLRGFLAALKVAGIMLLGGYGGVVWAQQTVPSSMAAL
                     IISIEPLWFVIFDWLFFKSRKPSTAEALGVALGFGGTVFLVLSQSGYSLSLKGEYTVG
                     MLVVLVATFNWSMGALYSRKAPIAKSSLLAAGMQMAAGGFLLLLVSALTGEPSSLSMD
                     AVSLK"
     gene            1001..>1478
                     /locus_tag="P5201_13175"
     CDS             1001..>1478
                     /locus_tag="P5201_13175"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain-containing protein"
                     /protein_id="HRV99543.1"
                     /translation="MLPKKMVDLDRVIASSPVVVLWGEPLDHRPVDFVTDNVSQFGYA
                     VPEMVLEEVRYDSIVFDEDRERVRSAFQNALLDGSDSFILDYRIITRAGAVRWVEDRV
                     AFHRDETGKVCLYQSSLLDITERKTAELQLRESEERLARVLVSARVGVWEYLPESDE"
BASE COUNT          337 a          392 c          456 g          293 t
ORIGIN      
        1 tttgagcgat acagcgtcca tactgaggct ggaaggctcc ccggtgagcg cggagacgag
       61 gagaagcagg aagcctccgg cggccatctg cattcccgcg gcaagcaggc tggacttcgc
      121 gatcggggcc ttgcgcgagt agagcgctcc catgctccag ttgaatgtgg cgacgaggac
      181 gaccagcata ccgacggtgt actctccctt cagcgagagc gagtaccccg actgcgagag
      241 gacgagaaag accgtaccgc cgaatccgag ggcgacgccg agcgcctcgg ccgtcgacgg
      301 cttgcgcgat ttgaagaaga gccagtcgaa gatgacgaac cagagcggtt cgatggagat
      361 gataagggcc gccatggacg agggaactgt ctgctgcgcc cataccacgc cgccgtaccc
      421 tccgagcagc atgatcccgg caactttcaa tgcggcgagg aatcctcgca gcgtggggcg
      481 ctcctgttcc ctgtacagac accagacgag cagaatgccc ccggcgagga agaagcggag
      541 agagccgctg aggaacggcg gtatcgtctc caccgtaaag cggatggcca gatacgtgga
      601 accccagatg atatagagcg ccgcgtaggc ggagaagtag agcagcggag acgtggtgcg
      661 agcggacatg acaataaccc ccagaatgta ttcgaatcgc ggcgatatta tagcagatag
      721 ttccttctta caacatcctc gattgaaaaa agcagatttt taagagggaa ttgccggtcc
      781 ggtgtttctc ctccgttcca acctgcttcc cggcggcgcg ctcttcagcg gcgagcgcgt
      841 tggctgctct tttcgcccgc ggatttacgg gtgaaatgct ccctgttcaa agctgaagaa
      901 ttcgcgtaaa ataaaaaatg caatgtgata agttgattct ttgtccgcat gcgtttttgt
      961 gctgttgacc tgttttttcc aagagcgaag gagatggagc atgcttccga agaagatggt
     1021 cgatcttgat cgggtgatcg catcaagccc ggtggtggtc ctctggggcg agccgctgga
     1081 tcatcgtccc gtggacttcg taacggacaa cgtgagccag ttcggatacg ctgtgcccga
     1141 gatggtgttg gaggaagtcc gatatgactc cattgtgttc gacgaggaca gagaacgtgt
     1201 gcgttcagcc tttcaaaacg cattgctgga cgggagcgac tccttcatcc tcgactatag
     1261 aatcatcacc cgcgccggag cggtccgctg ggttgaggac cgggttgcat tccaccgcga
     1321 cgaaacaggg aaagtatgtc tctatcaatc aagccttctg gatatcacgg aaagaaaaac
     1381 ggcggagctt cagcttcgcg agagcgagga acgtcttgcc cgcgtgcttg tctccgccag
     1441 ggtcggcgtg tgggagtatc ttcccgaaag cgatgaac
//
LOCUS       DAOTIL010000780         1478 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminobacteriaceae bacterium isolate AMR_MDS_3026
            NODE_102590_length_1478_cov_2.853830, whole genome shotgun
            sequence.
ACCESSION   DAOTIL010000780 DAOTIL010000000
VERSION     DAOTIL010000780.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868050
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminobacteriaceae bacterium (wastewater metagenome)
  ORGANISM  Aminobacteriaceae bacterium
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae.
REFERENCE   1  (bases 1 to 1478)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1478)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:19:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,224
            CDSs (total)                      :: 3,198
            Genes (coding)                    :: 3,184
            CDSs (with protein)               :: 3,184
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1478
                     /organism="Aminobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_102590_length_1478_cov_2.853830"
                     /isolate="AMR_MDS_3026"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:3034609"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(288..455)
                     /locus_tag="P5201_13180"
     CDS             complement(288..455)
                     /locus_tag="P5201_13180"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRV99544.1"
                     /translation="MAQYEPFTYETLDELRRKIESLGLDISFSEDLSVLAAPAAVGGR
                     TAPNRFSTLPM"
     gene            complement(652..1395)
                     /locus_tag="P5201_13185"
     CDS             complement(652..1395)
                     /locus_tag="P5201_13185"
                     /inference="COORDINATES: protein motif:HMM:NF019349.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FadR/GntR family transcriptional regulator"
                     /protein_id="HRV99545.1"
                     /translation="MNESRKILVEKVYAAIKDGSIVKDGKLPTEREMAALMTTSRTSM
                     REALIVLETLGIIEVKGKQGLFVKDAGMGRLNQSLDLYATWPADIIPQIFQVRIMMES
                     PAAGLAARNRTDADLAKMAECLAQFSRIFGEDGPDAGREGAHWNDIFHRIVIAASHNE
                     VLIRMHEGLSSIIERAMESLNKNRLTTPRPQWTERILGEHEQIVRAIRERDETGARES
                     MRKHLEISAANLEKLCQDKESALLGLPVG"
BASE COUNT          251 a          486 c          448 g          293 t
ORIGIN      
        1 gtccccgtcg cgtgccggta acgccccgca tcctgaagct gaagcagaaa aagaggcgaa
       61 tgcgacggac cgaagggttc gcgggccgcg gcgcgcgccg cttcgagcag agaggcgatc
      121 gccttttcgg tcgagggttg gatgcacatc tgacggtcgc tgctgcgccc cccctgccag
      181 acggcgcacg cctcgaactg gatcagcccc catccccccg cgccgtagcg gcggtagcgc
      241 cggaaggtaa gttctccggg ggaaccgtcg gggagcgcgt cgcgccccta cataggaagc
      301 gtcgagaagc ggttgggagc agtcctccct ccgaccgccg cgggcgcggc gaggacggag
      361 agatcctcgc tgaaggaaat gtcgagaccg agcgattcga tcttccgccg aagctcgtcc
      421 agtgtttcgt aggtgaatgg ttcgtactgt gccatgggcc tccctctcta tgacgggcct
      481 tccgtgggaa ggctccggat tcaattcttg ggttgaccaa ggacaaccaa aactgacgca
      541 attataccat ttgctcctca gattgagaag ccccggagcg tttcaaatac cctcaggccg
      601 gaaagcctcc gccggaaccg gcaaaccggg agaaaagagc gctgttcgtt actatccgac
      661 gggtagtccc agaagcgccg actccttgtc ctgacagagc ttttccaggt ttgcggcgga
      721 gatctccaga tgcttccgca tcgactcgcg cgcgcccgtc tcgtcccgct cgcggatcgc
      781 ccggacgatc tgctcgtgct cgcccagaat gcgctccgtc cactgcggac gcggcgtggt
      841 gaggcggttc ttgttcagcg attccatcgc gcgctcgatg atcgaggaga gcccctcgtg
      901 catccggatg agcacttcgt tgtgggaggc cgcgatgacg atgcggtgga agatgtcgtt
      961 ccagtgggcc ccctcgcgcc ccgcgtcggg cccgtcctcg ccgaagattc gcgagaactg
     1021 cgcgaggcac tccgccatct tcgccaggtc cgcgtccgtc cggttgcgcg ccgcgagccc
     1081 cgcggccgga ctctccatca tgatgcgcac ctggaagatc tgggggatga tatccgccgg
     1141 ccacgtcgcg tacaggtcta gactctggtt gagccgcccc atgcctgcgt ccttcacgaa
     1201 gagcccctgc ttccccttca cctcgatgat cccgagcgtc tccagcacga taagcgcctc
     1261 gcgcatcgac gtcctgctgg tggtcatcag cgcggccatc tcgcgctccg tggggagctt
     1321 gccgtccttt acgatggagc cgtccttaat cgcggcgtac accttctcca ccagaatttt
     1381 tctcgattcg ttcatttctt cctccggttt cgactccccg tctttcggcc gagatgctat
     1441 tatagagacg cgccaagggg agtatggttc cacttatg
//
LOCUS       DAOTIL010000781         1477 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminobacteriaceae bacterium isolate AMR_MDS_3026
            NODE_102697_length_1477_cov_2.823488, whole genome shotgun
            sequence.
ACCESSION   DAOTIL010000781 DAOTIL010000000
VERSION     DAOTIL010000781.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868050
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminobacteriaceae bacterium (wastewater metagenome)
  ORGANISM  Aminobacteriaceae bacterium
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae.
REFERENCE   1  (bases 1 to 1477)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1477)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:19:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,224
            CDSs (total)                      :: 3,198
            Genes (coding)                    :: 3,184
            CDSs (with protein)               :: 3,184
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1477
                     /organism="Aminobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_102697_length_1477_cov_2.823488"
                     /isolate="AMR_MDS_3026"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:3034609"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..207
                     /locus_tag="P5201_13190"
     CDS             <1..207
                     /locus_tag="P5201_13190"
                     /inference="COORDINATES: protein motif:HMM:NF024773.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-binding protein"
                     /protein_id="HRV99546.1"
                     /translation="GVDVVALFLSEKMQGTIAEDLKQLSYKPVVWFQPGAQNEPLANS
                     LAGAGYPVVEEDCLMAAHMNKCGV"
     gene            340..765
                     /locus_tag="P5201_13195"
     CDS             340..765
                     /locus_tag="P5201_13195"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006300605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioesterase family protein"
                     /protein_id="HRV99547.1"
                     /translation="MSSEIPFTLRVRYGDTDQMGVAYYANYLYWFEVGRTEFCRALGR
                     SYADWEAEGVFLPVVESHCRYKHPARYEDEIVIYTKIGEIRASSIVFECRIERASDGK
                     PLAAGWTRHAFVDADGRLIRGDNPMRRWIEALRDDGGVA"
     gene            749..>1477
                     /locus_tag="P5201_13200"
     CDS             749..>1477
                     /locus_tag="P5201_13200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013048911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="indolepyruvate ferredoxin oxidoreductase subunit
                     alpha"
                     /protein_id="HRV99548.1"
                     /translation="MEVSRRVSTKAILLGNEAIARGIVEAGCLVASAYPGTPSSEILP
                     AVAKFADEMKSPMSVEWSVNEKVAFEVAAAASFTGARACAVMKQVGLNVAADPFMSVA
                     HFDLKGGFLLVVADDPGPHSSQTEQDSRYFAMFAKIPCFDPATAAEAKEMVFDAFDLS
                     ERFGIVAMLRPGVRVDHCRQDIELGDIRPATGTVKFEKEGTRWVCLPAGVKINHPKLN
                     RKVEDIRKAFETEFSKYNYEVPAKK"
BASE COUNT          298 a          382 c          482 g          315 t
ORIGIN      
        1 ggtgtcgatg ttgtcgcgct ctttctctcc gagaagatgc agggtacgat tgcggaggac
       61 ctgaaacagc tctcctacaa gcctgtggtg tggttccagc cgggagcgca gaatgagccg
      121 cttgcaaact cgctcgccgg ggcggggtat cctgtggtgg aagaagactg tcttatggcc
      181 gcgcatatga ataagtgcgg cgtatgattt tgttgttgcg taaaaaatag tatttggtat
      241 ataattcacc acaaggagtg agttcttgtt gaaaacgcgc gcatcacatg cgtttttcaa
      301 gatgtttgtt tcactccacc ttttccggga ggtgttcccg tgtcctccga aattcctttc
      361 acactgcgcg ttcgttacgg cgataccgac cagatgggcg tcgcgtacta cgccaactat
      421 ctctactggt tcgaggtcgg gcggacggag ttctgccggg ctcttggcag gtcgtacgcc
      481 gactgggagg ccgagggagt attcctgccc gtcgtcgagt cgcactgccg gtacaagcat
      541 cctgctcggt acgaggatga gatcgtcata tacacgaaaa tcggtgaaat acgggcgagc
      601 agcatcgtct tcgaatgtcg catcgaaagg gcgtccgacg ggaaacctct tgccgccggg
      661 tggacccgcc atgcgtttgt cgacgcggac ggcaggctga tacggggaga caaccccatg
      721 cgccgctgga tagaggcgct gagggatgat ggaggtgtcg cgtagagtga gcacaaaagc
      781 catactgctt ggaaacgaag cgatagcgag aggaattgtg gaggccggat gcctggttgc
      841 gtcggcctat ccggggacgc cgtcgtcgga gattctgccg gcggtggcga agttcgccga
      901 cgagatgaag tcgccgatgt cggtggagtg gagcgtcaac gagaaggtgg cgttcgaggt
      961 ggctgctgcg gcttcgttta ccggcgccag ggcatgcgcg gtgatgaagc aggtcgggct
     1021 caacgtggcg gccgatccgt tcatgagcgt ggcgcacttc gacctgaagg gcggtttcct
     1081 gctggtcgtc gcggacgatc cgggtccgca cagctcgcag acggagcagg acagccgcta
     1141 cttcgccatg ttcgccaaga tcccgtgctt cgaccctgcg accgccgccg aggccaagga
     1201 gatggtcttc gacgccttcg acctctcgga gcgttttggc atcgtcgcga tgctccggcc
     1261 gggcgtgcgc gtggatcact gccgtcagga tatcgaactt ggagatattc gccctgcaac
     1321 cggtacggta aagttcgaga aagagggaac ccgctgggtc tgtcttcccg caggagtgaa
     1381 gatcaaccat ccgaagctca accgcaaggt tgaagatatc cggaaggctt ttgaaaccga
     1441 gttctcgaag tataactacg aggttccggc gaagaag
//
LOCUS       DAOTIL010000782         1474 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminobacteriaceae bacterium isolate AMR_MDS_3026
            NODE_102953_length_1474_cov_3.420719, whole genome shotgun
            sequence.
ACCESSION   DAOTIL010000782 DAOTIL010000000
VERSION     DAOTIL010000782.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868050
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminobacteriaceae bacterium (wastewater metagenome)
  ORGANISM  Aminobacteriaceae bacterium
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae.
REFERENCE   1  (bases 1 to 1474)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1474)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:19:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,224
            CDSs (total)                      :: 3,198
            Genes (coding)                    :: 3,184
            CDSs (with protein)               :: 3,184
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1474
                     /organism="Aminobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_102953_length_1474_cov_3.420719"
                     /isolate="AMR_MDS_3026"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:3034609"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..365
                     /locus_tag="P5201_13205"
     CDS             <1..365
                     /locus_tag="P5201_13205"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRV99549.1"
                     /translation="GLAHRATRWQISTENTFSSAESGTSSSLTFDLTSTEDLLELYVP
                     TGALAQGTTYYWRTRFQDTERGWSGWSAVRSFTTLSAPPAPTPSTGGSGGCSLHAGNG
                     LTAVLLLVPLTLLGRKAR"
     gene            complement(799..>1474)
                     /locus_tag="P5201_13210"
     CDS             complement(799..>1474)
                     /locus_tag="P5201_13210"
                     /inference="COORDINATES: protein motif:HMM:NF014017.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF72 domain-containing protein"
                     /protein_id="HRV99550.1"
                     /translation="EVPRPESGRLDFQGLPKETRPLLWRRFTESLVPLRQMNRMGYLL
                     FQLPPKAVFSPVMMRYLERVAEVTPPFRVAVEVRSRSWMEPGNRESFLDLLKGANMAY
                     VAVDEPELPWTVGRDAPVTASWGSVVRFHGRNTEAWSRKDATVAERFRYCYSDAEMLS
                     WEGAVRDAATKAERLFLMFNNCFRDYAVQNAQRMKCLLGVACAPGEGVQGELDFEGFA
                     DESKIE"
BASE COUNT          315 a          439 c          386 g          334 t
ORIGIN      
        1 agggattggc ccatagggcg acacgctggc aaatttccac cgagaacaca ttctcgtccg
       61 ccgaaagtgg tacgtcatcg tctctgacct ttgatcttac cagtacggaa gacctgctgg
      121 aactgtacgt acctacaggc gccctggccc agggaacgac ctactactgg cgcacacgct
      181 tccaggatac ggagcggggt tggtccgggt ggtccgcggt acgaagcttt acaacgctct
      241 ccgccccgcc ggcgcccact ccctcaaccg gaggcagcgg aggatgctcg ctgcacgcag
      301 ggaacggact gacggcggtt ttactgctcg ttcctctgac gctgctcggg agaaaagcgc
      361 ggtagataac gaaaggatga ggacgcaacg atatctgcgt cctcatcctt aacaggcgta
      421 cagtattaaa tgcatacgaa aagggagggg aaatattccc ctcccggact ttcaacgggc
      481 tatctcggac ggtggttctg accttctgtc aaacaaccag ctcctagtac ttctgtagat
      541 atcccacacg tatctttgtt gaggttctgt acagctttcc ttgttgcggc ggttctttgg
      601 ttcgtggtcc tcgtttcagt tgctgtcttt ggtgatcggc tttcctgact tgaaactctt
      661 tgatgcttct tctctccgtc cggataaccc gttaggagta ttctacactt gcacgctgca
      721 tccgtcaaac agacagagta tacagcctcg gagatataat ccttatttta agccgggaat
      781 gctgtttttc ttcatttttc attcaatttt gctctcatcc gcgaagcctt caaaatccaa
      841 ctccccctgc accccttctc ctggagcgca ggcgacgccg agcaagcatt tcattcgctg
      901 cgcgttctgc accgcgtaat ctctgaaaca gttgttgaac atcaggaaga ggcgctccgc
      961 ttttgtcgcg gcgtcacgga ccgcgccctc ccaggagagc atctccgcat ccgaataaca
     1021 ataccggaat cgctccgcga cggttgcatc tttgcggctc catgcttccg tattgcgtcc
     1081 gtggaagcgg acgaccgaac cccaggaagc ggtaacggga gcgtcccttc ccacggtcca
     1141 gggcagttca ggttcgtcca ccgcgacgta cgccatattc gcccctttca gcaaatccag
     1201 gaacgactcc cggtttcctg gttccatcca ggaacggctc cgaacctcga ccgcgacgcg
     1261 gaagggcgga gtcacctcgg caacgcgttc gagatagcgc atcatcacgg gggaaaaaac
     1321 agccttggga ggaagttgaa agagcagata ccccatacgg ttcatctgcc gcagcggtac
     1381 gagcgactcc gtaaaacgcc gccaaagcag ggggcgggtt tctttcggaa gcccctgaaa
     1441 atcaagcctc ccagactctg gccgggggac ttcg
//
LOCUS       DAOTIL010000783         1474 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminobacteriaceae bacterium isolate AMR_MDS_3026
            NODE_103004_length_1474_cov_2.313601, whole genome shotgun
            sequence.
ACCESSION   DAOTIL010000783 DAOTIL010000000
VERSION     DAOTIL010000783.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868050
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminobacteriaceae bacterium (wastewater metagenome)
  ORGANISM  Aminobacteriaceae bacterium
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae.
REFERENCE   1  (bases 1 to 1474)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1474)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:19:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,224
            CDSs (total)                      :: 3,198
            Genes (coding)                    :: 3,184
            CDSs (with protein)               :: 3,184
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1474
                     /organism="Aminobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_103004_length_1474_cov_2.313601"
                     /isolate="AMR_MDS_3026"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:3034609"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..814)
                     /locus_tag="P5201_13215"
     CDS             complement(<1..814)
                     /locus_tag="P5201_13215"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HRV99551.1"
                     /translation="MGSMLEQHILRTPLIGYSFRRIILDDSGKPVDYEFLEVNEAFER
                     FTGLARTDILGRTASKVVPGIESASFDWLGVYGEVALHGGSKEFEQYSELLGRWFRVD
                     AYSDRKYYFTVLFLDVTENKKQAEELADEAFRRSLLMEQSRDGIVILDENGGVYEANR
                     KFAEMLGRPFESIRRLTVFDWEFLHPRERLIDMLRSVDEKGDHFETQHRRRDGSVFDV
                     EISTNAAWFGGRKLIFCVCRDITERKRAEKENSIFKTIADSALYGQAIADLEG"
     gene            complement(953..1408)
                     /locus_tag="P5201_13220"
     CDS             complement(953..1408)
                     /locus_tag="P5201_13220"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HRV99552.1"
                     /translation="MSRIEKTPTIMIVDDTPAILDLLDQMLRAQGYRALTFPRGKLAL
                     EAAEETPPDLILLDITMPEMDGFEICRRLKNTPRLAEIPVIFIGPLAEPDEKALAFAA
                     GGADYVTKPFQEEEILVKVRTYLRMMEMQRELRKRWKTTETPSGEKAPR"
BASE COUNT          269 a          432 c          422 g          351 t
ORIGIN      
        1 tgccctcaag atcggcgatg gcctggccgt agagcgcgct gtcggcgatc gtcttgaata
       61 tactgttttc tttctcggcg cgcttgcgtt ccgtgatatc ccggcagacg cagaagatca
      121 gcttccgccc gccgaaccag gcggcgttgg tgctgatttc gacgtcgaag acgctgccgt
      181 ccctgcgccg gtgctgtgtt tcaaagtggt cgcccttctc gtcgacgctc cgcagcatgt
      241 cgataaggcg ttcccggggg tgcagaaact cccagtcgaa gaccgtgagg cgtcgtatgg
      301 attcgaaggg gcgcccgagc atttcggcga attttctgtt ggcttcgtac actccgccgt
      361 tctcgtcgag gatgacgatc ccgtctctgg actgctccat gaggagactc ctccggaacg
      421 cttcgtccgc gagttcctcc gcctgttttt tattttccgt gacatcaagg aaaaggacgg
      481 tgaagtagta cttccggtcg gagtaggcgt ccactcgaaa ccatctgccg aggagctccg
      541 agtactgctc gaactcctta gaaccgccgt gcagcgcaac ctctccgtac acgccgagcc
      601 agtcgaagga ggcgctttcg atgccgggaa cgaccttgct tgcagtgcgc cccagaatat
      661 cggtgcgcgc aagcccggtg aatctctcga aggcctcgtt cacctccaga aattcgtagt
      721 cgaccggctt gcccgaatcg tcgaggatga tccggcgaaa tgagtacccg atcagagggg
      781 tgcgcagtat gtgctgttcg agcatcgaac ccatgaatac acctccgtgc cgcgttggcc
      841 tgcttcggcg gaaacggcgc gccggaaaga gtacggctcg ccgttttttt ttcatgattc
      901 tattgtatca gtggcgccgc gtgcacattc tattttcttc gaatagcgga gcttaccggg
      961 gcgctttttc tccggagggc gtttcggttg tcttccaccg ctttcgaagc tcgcgctgca
     1021 tctccatcat ccgaagatat gtccgtacct ttaccagaat ttcctcctcc tgaaaaggct
     1081 tcgttacgta gtccgcgccg cctgcggcga aggccagtgc cttttcgtcg ggttcggcga
     1141 gcggcccgat gaagatcacc ggaatctccg cgagacgagg cgtgtttttc aatcgcctgc
     1201 agatctcgaa tccgtccatt tcgggcatcg tgatatcgag caggatcaga tccggagggg
     1261 tttcctccgc ggcttcgagc gccagttttc ctcgcggaaa ggtcagggcg cggtacccct
     1321 gcgcccgcag catctgatcc agcaggtcga gaatagcggg agtatcgtcc acgatcatga
     1381 tggtcggcgt cttttcgatg cggctcattt ccctctgtac ctcctagaat aaagtagttg
     1441 caactgggat cagcaaatag cccgtagagt tgcg
//
LOCUS       DAOTIL010000784         1472 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminobacteriaceae bacterium isolate AMR_MDS_3026
            NODE_103174_length_1472_cov_3.208892, whole genome shotgun
            sequence.
ACCESSION   DAOTIL010000784 DAOTIL010000000
VERSION     DAOTIL010000784.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868050
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminobacteriaceae bacterium (wastewater metagenome)
  ORGANISM  Aminobacteriaceae bacterium
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae.
REFERENCE   1  (bases 1 to 1472)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1472)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:19:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,224
            CDSs (total)                      :: 3,198
            Genes (coding)                    :: 3,184
            CDSs (with protein)               :: 3,184
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1472
                     /organism="Aminobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_103174_length_1472_cov_3.208892"
                     /isolate="AMR_MDS_3026"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:3034609"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..359)
                     /locus_tag="P5201_13225"
     CDS             complement(<1..359)
                     /locus_tag="P5201_13225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012870063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HRV99553.1"
                     /translation="MKVMIVDDAAFMRMMIRKHLEGAGHEVVAEAFEYHSAMDEFKKL
                     LKTGTLPDAITMDITLPGRRSGIDITREIHRLAPEVRIVMTSAMGTQHMVIDAMKAGA
                     TDFVVKPFKPEQLLAAL"
     gene            645..>1472
                     /locus_tag="P5201_13230"
     CDS             645..>1472
                     /locus_tag="P5201_13230"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRV99554.1"
                     /translation="MLHDALAELPPGCAPACGACERPRSVEESLAGKKARAIRALSRW
                     EDRIEDIVSPPEERRLGYRDRTALKALWTPEERWRFGMEGVRTDVRGPLRFARPRPFV
                     PLTDCPVHTERIRKMLHLPASALPPDEPEGSFPLSFLVVSGAQATLVFKAKKTPSDNW
                     FFEPRDGRPSLADALGDIGIEGLWFHANPAAGVRLFAKRGWRLAWGAPRSADSRGLLY
                     GPASFSQLLPELHDASLDEARRFLVPGAGDAAADLYCGIGASLRLWRKCGADALGVEG
                     "
BASE COUNT          251 a          499 c          440 g          282 t
ORIGIN      
        1 tccagcgcag cgaggagctg ttccggcttg aacggcttga cgacgaaatc ggtcgctccc
       61 gccttcatcg cgtcgatcac catgtgctgc gttcccattg cggacgtcat cacgatcctg
      121 acctccggag ccaacctgtg aatttcgcgc gtaatatcta tccccgaccg tctgccggga
      181 agcgtaatgt ccatcgtgat cgcatcggga agcgtaccgg tcttcagcag ttttttgaac
      241 tcgtccatcg ccgagtggta ttcgaacgcc tccgcgacga cttcgtgccc cgctccctcc
      301 aggtgttttc gtatcatcat gcgcatgaat gcggcgtcgt ccacgatcat cactttcatt
      361 cccgtaccct ccgtcgaacg agtcattctc atgctttcat cgaagcaaaa aaacaatacc
      421 cagttaacat agtatagcct cttcgaagga aattcgcacc cccgaaaatc gattcgacag
      481 cgccgcttcg atacggagga cgccgagggt ccggcaattc cgagagatcg cggcgacgaa
      541 agtacgcaca ctctctttct ccgtgctgtt cgattgcagt ctggcgcgct tgcagcgcgt
      601 acggtatgct tgagcgagaa cacacgaaga cgaggtgacc atacgtgctc catgacgctc
      661 ttgccgaact tcctcccggg tgcgcccccg cctgcggcgc gtgcgaacgg ccgcgctccg
      721 tcgaggagtc gctcgccggg aagaaggccc gcgccatccg cgccctctcc cgctgggagg
      781 atcgcatcga ggacatcgtg tcgcccccgg aggaacgacg cctggggtac cgtgaccgaa
      841 ccgccctgaa ggcgctttgg acgcctgaag aaaggtggcg tttcggcatg gagggcgtcc
      901 ggacggacgt tcgcggcccg ctccgcttcg cgcgtccccg ccccttcgtc ccgctcaccg
      961 actgcccggt gcataccgaa cgaatccgga aaatgctgca cctgccggcg tccgccctcc
     1021 cgcccgacga accggagggg agcttcccac tcagctttct cgtcgtctcc ggcgcacagg
     1081 cgacgctcgt gttcaaagcg aaaaagacgc cgtcggacaa ttggttcttc gagccgcggg
     1141 acggccgccc ctcccttgcc gacgcgctcg gtgacatcgg aatcgaaggg ctctggttcc
     1201 acgccaatcc tgcggcggga gtgcgcctct tcgccaagcg ggggtggcgg ctcgcatggg
     1261 gcgcgccccg ctccgccgac tcacgggggc tgctctacgg accggcgtct ttctcgcagc
     1321 ttctgccgga gctgcacgac gcgtcgctcg acgaagcgcg ccgcttcctc gttccggggg
     1381 cgggtgacgc tgcagccgac ctctactgcg gcatcggcgc aagtttgcgc ctttggcgga
     1441 aatgcggggc ggacgcgctc ggcgtggagg gg
//
LOCUS       DAOTIL010000785         1471 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminobacteriaceae bacterium isolate AMR_MDS_3026
            NODE_103258_length_1471_cov_4.173729, whole genome shotgun
            sequence.
ACCESSION   DAOTIL010000785 DAOTIL010000000
VERSION     DAOTIL010000785.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868050
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminobacteriaceae bacterium (wastewater metagenome)
  ORGANISM  Aminobacteriaceae bacterium
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae.
REFERENCE   1  (bases 1 to 1471)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1471)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:19:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,224
            CDSs (total)                      :: 3,198
            Genes (coding)                    :: 3,184
            CDSs (with protein)               :: 3,184
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1471
                     /organism="Aminobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_103258_length_1471_cov_4.173729"
                     /isolate="AMR_MDS_3026"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:3034609"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1070)
                     /locus_tag="P5201_13235"
     CDS             complement(<1..1070)
                     /locus_tag="P5201_13235"
                     /inference="COORDINATES: protein motif:HMM:NF017633.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NFACT family protein"
                     /protein_id="HRV99555.1"
                     /translation="MSFGPELITVLAREVNAREMPARIHKIETGDVWAALKIGGEKWL
                     FFSWHAVHYGLGIVGEETVAALKKMKTPKSSFGEALKRNFLNGTVLSAKQLYNDRILL
                     LETERLVGAGFTVSNFLIFEGMDRNSNLILLDEKQCVLDAAKHIHPDVNRYRTVLPGN
                     HYTPPPPRDGAVWSTKREHLSLLDIRTLIGIGRPLVKTIIEHWESFPLSFWSEAIDGL
                     LGGAPSALPLGLQRIGNYITVFPVLFKDAEALDGSPIERCGKETAGKLLSLHREKGLA
                     SVRKAIEKEIRSRERHADGLRRQLSLSGHAEEFKKYGELLLGSAHCIRQGADTFTVTD
                     WETQREITIVLDEKLSPTANAER"
     gene            1304..1378
                     /locus_tag="P5201_13240"
     tRNA            1304..1378
                     /locus_tag="P5201_13240"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1336..1338,aa:Ala,seq:cgc)
BASE COUNT          330 a          390 c          391 g          360 t
ORIGIN      
        1 cgctccgcat tcgctgtcgg cgagagtttt tcgtccagaa cgatcgtgat ttcccgttgc
       61 gtctcccagt ctgtaacggt aaacgtgtct gctccctgcc ggatacaatg cgcggagccg
      121 agaagaagct ctccgtactt tttaaactct tcggcgtgcc cggaaagtga gagctgtctg
      181 cgtaatccgt cagcatggcg ttcacggctt cgtatctctt tttctattgc tttgcgtacg
      241 gatgcgaggc ctttttcccg gtgcagcgag aggagcttcc cagcggtttc tttcccgcag
      301 cgttcaatcg gacttccatc cagtgcttct gcgtctttga aaagcaccgg gaagactgtt
      361 atataattac cgatcctctg gagaccgagg ggaagtgcgc tcggcgcgcc gccaagcaat
      421 ccgtcgattg cctcagacca aaaggagaga gggaagctct cccaatgctc aattatggtt
      481 ttaacaagag gtctgccgat accgatgagg gtacgtatat cgagaaggga aaggtgctct
      541 cttttcgtac tccacacagc cccgtcccgc gggggagggg gcgtgtagtg attccccggc
      601 aatactgtac ggtaccggtt cacatccgga tgaatgtgtt tcgctgcatc caggacgcac
      661 tgtttttcgt cgagaagaat caggttgctg ttacgatcca tcccttcgaa aatcaaaaaa
      721 ttcgagacag tgaacccggc gccgacaagg cgttcggttt ccagcagcag aatgcggtcg
      781 ttgtatagtt gttttgccga aagaactgtt ccattgagaa agtttctttt cagggcttcg
      841 ccgaatgacg acttgggcgt cttcattttc ttcagtgcag caacagtttc ttccccgacg
      901 atacccagtc cgtaatgaac agcatgccac gaaaagaaga gccatttttc accgcctatc
      961 ttcagtgcgg cccacacatc gcccgtttca atcttatgta ttcgcgccgg catttcgcgt
     1021 gcattgactt cacgcgcaag aaccgtaatg agttccggac cgaaagacac gagcatcact
     1081 ccgttttcag atcaaaattc tttcaggggt aaaagtatac cacttccccg gatctccagg
     1141 agaaatttcg cctctggcag tggaatatac ccagaaggcc ggtgcaggaa aggttccggc
     1201 attcaaaaca gctgtcggta aaccgaaagt cgaaattctc cacgaaagtc gaggcttgac
     1261 aggaacggga tcgccgtata aaataatcgc cgttggtctg accggggctg tagcgcagtg
     1321 ggagcgcgct tccctcgcac ggaagaggcc gggggttcga atccccccag ctccaccaga
     1381 tttcgctcgg ttagttcgat tgcttctgta caatgaatcg gggcgtagcg cagtctggtt
     1441 agcgcacctg ctttgggagc agggggtcga a
//
LOCUS       DAOTIL010000786         1470 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminobacteriaceae bacterium isolate AMR_MDS_3026
            NODE_103377_length_1470_cov_3.491166, whole genome shotgun
            sequence.
ACCESSION   DAOTIL010000786 DAOTIL010000000
VERSION     DAOTIL010000786.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868050
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminobacteriaceae bacterium (wastewater metagenome)
  ORGANISM  Aminobacteriaceae bacterium
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae.
REFERENCE   1  (bases 1 to 1470)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1470)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:19:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,224
            CDSs (total)                      :: 3,198
            Genes (coding)                    :: 3,184
            CDSs (with protein)               :: 3,184
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1470
                     /organism="Aminobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_103377_length_1470_cov_3.491166"
                     /isolate="AMR_MDS_3026"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:3034609"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..235
                     /locus_tag="P5201_13245"
     CDS             <1..235
                     /locus_tag="P5201_13245"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRV99556.1"
                     /translation="WESERIREEIVAEGGTPMLVKGPFGVSVSAEQERLCRLVVRMED
                     ATVCVAEEPSEALASFFPSAAGWTIQKFPAGQK"
     gene            complement(242..886)
                     /locus_tag="P5201_13250"
     CDS             complement(242..886)
                     /locus_tag="P5201_13250"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013672.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HRV99557.1"
                     /translation="MRAAFLLLLVFFAIPSFAEASGYLRNGNGHTVALTFDDGPRPGY
                     VEPILAILREKDVRATFFLVGRYVLEHPELVRAIDAEGHTVANHTFYHNNLTSLPPEN
                     VYREWRLCNEAVEQVTGKKPRFARPPGGRYNNFVAERANEEGLRIVLWTNNPGDYAAS
                     LDGPSLARKVLSRAREGDIVLLHVGVAPTIDALPEIIDGYRRKGFSFVTVDEMR"
     gene            1281..>1470
                     /locus_tag="P5201_13255"
     CDS             1281..>1470
                     /locus_tag="P5201_13255"
                     /inference="COORDINATES: protein motif:HMM:NF012610.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HRV99558.1"
                     /translation="MISYFDMSELKDGVTRQVYLALRRDILNWRLLPGRRLSEKDSAG
                     RMNISKTPVREAFIKLQEE"
BASE COUNT          309 a          459 c          418 g          284 t
ORIGIN      
        1 ctgggagagc gagcgcatcc gcgaggagat cgtcgccgaa ggagggacgc cgatgctcgt
       61 caaagggccc ttcggcgttt cagtatccgc cgagcaggag cggctctgcc gcctcgtcgt
      121 ccggatggag gacgccacgg tgtgcgtcgc ggaggagcct tccgaagcgc tggcctcctt
      181 cttcccctcc gccgccgggt ggacgattca gaagttcccc gccggtcaga agtaggcgaa
      241 gttaccgcat ctcgtcgacg gtgacgaagg agaacccttt cctacggtag ccgtcgatga
      301 tttccggcag cgcgtcgatc gtgggcgcga cgccgacatg cagcagcaca atgtcgccct
      361 ctctcgccct ggagagcacc ttccgggcga gagaggggcc gtcgagcgac gcggcgtagt
      421 cccccggatt gttggtccac aggacgatgc gcagcccctc ttcgttcgcc cgctccgcga
      481 cgaaattgtt gtagcgcccc ccgggcggcc gggcgaaccg cggttttttg ccggtaacct
      541 gctccacggc ctcgttgcag agccgccact cgcggtagac gttttccggc ggcagcgacg
      601 tcaggttgtt gtggtagaac gtatggttcg ccaccgtatg cccctccgcg tcgatcgccc
      661 gcaccagctc cggatgctcc agaacgtatc gtccgaccag gaagaacgtc gccctgacgt
      721 ccttctcccg cagaatagcc agtatcggct cgacgtaccc cggacggggg ccgtcgtcga
      781 aggtcagcgc caccgtatgc ccgttcccgt ttctcagata gcccgaggct tccgcaaacg
      841 aggggattgc gaagaagacg agcaaaagca aaaatgccgc tctcatttgt attctcttgt
      901 tcatagtcac ctttccattc ttctgaatgc gtcaaagagc acatgatagc actctccgca
      961 tcgtcgcgcc acacaatttt gccgaatccg cgggcagaaa gaggcatcac ccgcgtccga
     1021 atcaaacgac gcgaaactgt tttcacaaac ctcgccccga aaccacatgc aaagtcaatg
     1081 aaaaacttca aactccgggt gcgctgaaac tcgattcacc tctgaataaa aagtgaagaa
     1141 aatttcgttc gctccccttg acaaagacta aaacgatggc taacatacta acatatcagt
     1201 tttccatttt ctctagcgtg aattatgcga cacgcttcgg ctccccgccg gggcgatctt
     1261 cagggaaaga gggccttgcc gtgatctcgt atttcgacat gagcgaactg aaggacggcg
     1321 tcacgcggca agtctacctt gcgctccgca gggacatact gaactggcgt cttcttcccg
     1381 gaaggcggct cagcgagaag gacagcgccg ggcggatgaa catcagcaag acccccgtcc
     1441 gcgaggcgtt catcaagctt caggaggagg
//
LOCUS       DAOTIL010000787         1470 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminobacteriaceae bacterium isolate AMR_MDS_3026
            NODE_103402_length_1470_cov_2.775972, whole genome shotgun
            sequence.
ACCESSION   DAOTIL010000787 DAOTIL010000000
VERSION     DAOTIL010000787.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868050
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminobacteriaceae bacterium (wastewater metagenome)
  ORGANISM  Aminobacteriaceae bacterium
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae.
REFERENCE   1  (bases 1 to 1470)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1470)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:19:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,224
            CDSs (total)                      :: 3,198
            Genes (coding)                    :: 3,184
            CDSs (with protein)               :: 3,184
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1470
                     /organism="Aminobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_103402_length_1470_cov_2.775972"
                     /isolate="AMR_MDS_3026"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:3034609"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            324..>1470
                     /locus_tag="P5201_13260"
     CDS             324..>1470
                     /locus_tag="P5201_13260"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRV99559.1"
                     /translation="MMHIFFVHKKALIACLTILSLLALMPSTGAASAPAPNTFLSSPL
                     YGITHFDSSQSDSTPYGPPRGTFYIESSLQPISYGGPVNIITLASTDPEYMWAVGHDK
                     VSYVHRVSGEWKVVASFEALADASNGLLPAVPDEKFREFGMRNAIGMTVAQMDTYLTE
                     ILGENYKARFGNGAYSVVDKDNVLYTNFLSSLYAFGLKNPANPSEGIEVKRKIADVLK
                     DIQGVEGGESRPAVAPGPTRLFGLSMTYDGFLVVTFSNGVAVIQRDFDLASKVFYPFG
                     EDEYVSNSIAVDENNGMYIASNKLMRKLVWTKEKAISDKEEDGAWATPYDVADVLPPI
                     IKFDNGTGSTPTLMGFGDDSDKLVVITDGAKHMKLTAFWRDAIPEGFV"
BASE COUNT          318 a          418 c          403 g          331 t
ORIGIN      
        1 atgattttcg ctccattcta catcattgag attcttttct cttctgtcgg aagagcgtgc
       61 atcactcgct ctttttgcat ccggtaaaat gtgcgataat ctggtgtatc tactcccttt
      121 cgtgcagcga acccgttttc acccgtacgg aaggggttct tcgacgaaag gagggtgcga
      181 acgggcgcgg tgtttttgaa aagcgcacag tgccggctct ctgaatccgc ttcttcggaa
      241 cgttttcggg aggatctgcg tcgcttcgga tgaatgaacc cgggatactc gatgttcatc
      301 agaatttatt atcaggaggt tcgatgatgc acatattttt tgttcataag aaagcattga
      361 ttgcgtgcct gacaatactc tctcttctcg cgctcatgcc ttcaaccggg gcggcgagcg
      421 cccccgcccc caatacgttt ctgtcgtcgc ccctgtacgg cattactcat ttcgattctt
      481 cccagagcga ctccacgccc tacggccctc cccgcggcac gttctacatc gagtcgtcgt
      541 tacagcccat ctcttacggc ggaccggtga acatcattac gctcgcgtcc accgatccgg
      601 agtacatgtg ggctgtcggg cacgacaaag tgagctacgt ccacagggtg agcggagaat
      661 ggaaggtcgt cgcctcgttc gaggcccttg ccgacgcgtc gaacggcctc ttgcccgcag
      721 ttccggacga gaaattcagg gaattcggca tgaggaacgc aatcggaatg accgtggccc
      781 aaatggacac ataccttacg gaaattcttg gggagaacta caaggcgcgc ttcggtaacg
      841 gcgcctactc ggttgtcgac aaggacaacg tactctacac gaacttcttg tccagcctct
      901 atgctttcgg tttgaagaat ccggcgaacc cctccgaggg tatcgaagtg aagcgcaaaa
      961 tagcggatgt gctcaaggat atccaaggtg ttgaaggggg cgaaagcaga ccggcggttg
     1021 ctcccgggcc aacgcgcctc ttcggtctct ccatgaccta cgacgggttt ctcgtggtca
     1081 ccttcagcaa cggcgtggct gtcatacaac gcgatttcga cctcgcctcc aaggttttct
     1141 accccttcgg ggaggacgag tatgtgagca actccatcgc tgtggacgag aacaacggga
     1201 tgtacatcgc gagcaacaag ctgatgcgca agctggtctg gacaaaagag aaggctatct
     1261 ccgacaaaga ggaggacggc gcgtgggcta ccccctacga cgtggccgac gttttgccgc
     1321 cgatcatcaa gttcgacaac ggcacgggct ccacgccgac gctgatggga ttcggcgacg
     1381 actccgacaa actggtggtc atcaccgacg gcgcaaaaca catgaagctg acggcgttct
     1441 ggcgcgacgc aatccccgaa gggttcgtcc
//
LOCUS       DAOTIL010000788         1467 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminobacteriaceae bacterium isolate AMR_MDS_3026
            NODE_103703_length_1467_cov_2.873229, whole genome shotgun
            sequence.
ACCESSION   DAOTIL010000788 DAOTIL010000000
VERSION     DAOTIL010000788.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868050
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminobacteriaceae bacterium (wastewater metagenome)
  ORGANISM  Aminobacteriaceae bacterium
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae.
REFERENCE   1  (bases 1 to 1467)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1467)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:19:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,224
            CDSs (total)                      :: 3,198
            Genes (coding)                    :: 3,184
            CDSs (with protein)               :: 3,184
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1467
                     /organism="Aminobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_103703_length_1467_cov_2.873229"
                     /isolate="AMR_MDS_3026"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:3034609"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..236)
                     /locus_tag="P5201_13265"
     CDS             complement(<1..236)
                     /locus_tag="P5201_13265"
                     /inference="COORDINATES: protein motif:HMM:NF014707.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="epoxyqueuosine reductase QueH"
                     /protein_id="HRV99560.1"
                     /translation="MSAQKVLLHVCCAPDATVPWTDLPGEGYEVVPYWYGNNIHPAAE
                     EKRRRDTLAALSLRMGGSVLWEEYVPETWLEAASR"
     gene            complement(249..>1467)
                     /locus_tag="P5201_13270"
     CDS             complement(249..>1467)
                     /locus_tag="P5201_13270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009164265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="transketolase C-terminal domain-containing
                     protein"
                     /protein_id="HRV99561.1"
                     /translation="AVPTVILCRTVMGKGVPFMENSSEFHGKAPLNDLYVQAMRDLGG
                     DPSLLEDARNRRTSAQLPTGRHISTSSFGLDTGVPRIYTADAKKTDNRSAFGSALADI
                     GKLNYKKEGGAPLLVFDCDLAGSVKTGEFASLCPEWFIQTGIQEHATATVAGAASTAG
                     VAALWADFGVFGLSETYNQQRLNDINKANLKTVLTHVGLDVGEDGMTHQAIDYVSLLR
                     NTFGWKLAVPCDPNQTDRATRWAIKEPGCVCLAMGRSKLDVLTSAKGAPLFGEGYEFR
                     YGKAELVCEGNDGALFALGAMTGRALKAAQMLKEKGITVSVYAVSSPLLPDDDALRAA
                     AATGRIMTCEDHCVASGMGSILAARMTELRLNAAFKALGVHRYGDSGASEDVFAAMGL
                     DAASLAAAFEKLL"
BASE COUNT          285 a          446 c          422 g          314 t
ORIGIN      
        1 cgcgatgccg cttccagcca cgtttccggc acatactcct cccaaagaac actaccgccc
       61 atacggagag agagcgccgc cagcgtatcg cgccttcgtt tctcctctgc ggccgggtgt
      121 atattattgc cgtaccagta cggaacgact tcgtaccctt cgccggggag atccgtccac
      181 ggtacggtag catccggagc gcagcatacg tggagcaata ctttctgtgc gctcacggca
      241 aaaagctcct acagaagctt ctcgaaagcc gcggcaagcg acgccgcatc gagtcccatc
      301 gcggcgaaga cgtcctccga agcgccggaa tctccgtagc gatgcacacc aagcgcttta
      361 aatgctgcat tcagacgaag ttccgtcatc cttgccgcga ggatagagcc catgccgctt
      421 gcaacgcagt gatcttcgca cgtcatgatt cttcccgtgg cagccgcggc gcgaagtgcg
      481 tcgtcgtcgg gaagcaaagg tgaggagact gcgtaaaccg atacggtaat tcctttttcc
      541 ttcagcatct gcgcggcttt caacgctctt ccggtcatgg cgccgagagc gaagagagcg
      601 ccgtcgttcc cttcgcatac cagctccgct ttcccgtagc ggaattcata cccctccccg
      661 aagaggggcg cccccttggc gctcgtcagg acatcgagct tgcttcgccc catggcgaga
      721 cagacgcacc ccggttcctt gattgcccag cgtgtcgcgc ggtcggtctg gttcggatcg
      781 cacggaaccg cgagcttcca gccgaaggtg ttccgaagca aactgacgta atctatcgcc
      841 tggtgcgtca tgccgtcttc gccgacgtca agtccgacat gtgtgaggac tgttttcaga
      901 tttgccttgt tgatgtcgtt caggcgctgc tggttgtagg tctcggaaag tccgaagacg
      961 ccgaaatcgg cccagagggc cgcaactccg gctgtagagg ccgcccctgc aaccgttgcc
     1021 gttgcgtgtt cttggatgcc tgtctggatg aaccactccg ggcagaggga ggcgaattca
     1081 cctgttttca ccgaaccggc aaggtcgcaa tcgaagacaa gaagcggtgc gcctccttcc
     1141 ttcttgtagt tcagctttcc gatatcggcg agcgcggaac cgaaggcgga ccggttgtcg
     1201 gtcttcttcg cgtcggcggt atagatgcga ggtacgcccg tatccagacc gaaggaggaa
     1261 gtcgaaatat gcctccccgt tggaagctgg gctgaagttc ttctgttcct cgcgtcctcg
     1321 agcaacgacg gatcgcctcc gagatcgcgc atcgcttgga catagagatc gttgagaggc
     1381 gctttcccgt ggaattcgga gctgttttcc atgaaaggaa cgcccttgcc catgactgtc
     1441 cggcagagga taactgtggg tactgcg
//
LOCUS       DAOTIL010000789         1461 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminobacteriaceae bacterium isolate AMR_MDS_3026
            NODE_104342_length_1461_cov_2.846373, whole genome shotgun
            sequence.
ACCESSION   DAOTIL010000789 DAOTIL010000000
VERSION     DAOTIL010000789.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868050
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminobacteriaceae bacterium (wastewater metagenome)
  ORGANISM  Aminobacteriaceae bacterium
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae.
REFERENCE   1  (bases 1 to 1461)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1461)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:19:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,224
            CDSs (total)                      :: 3,198
            Genes (coding)                    :: 3,184
            CDSs (with protein)               :: 3,184
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1461
                     /organism="Aminobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_104342_length_1461_cov_2.846373"
                     /isolate="AMR_MDS_3026"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:3034609"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..951)
                     /locus_tag="P5201_13275"
     CDS             complement(<1..951)
                     /locus_tag="P5201_13275"
                     /inference="COORDINATES: protein motif:HMM:TIGR03882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TOMM precursor leader peptide-binding protein"
                     /protein_id="HRV99562.1"
                     /translation="MKTPAVAPHCRLQTVSGEGALALSERGAKAFYGAAYELVVPLID
                     GCRSADDIADALAGSVDAATVYYVLNILEQRGCVAEATPEIPPALSAFWHEAGVEPRA
                     AMEALRRSSVAVFVAGNLDSSPLETALKEAGVRTGPKESADLWVVVADDYQRDELREI
                     NGEALAARRPWLLLRPTGPEAWIGPLFLPGETACFRCLLSRLERNRSAHALAKRLGGG
                     KAVPSTSVAALLSTLASAYGMAATEAARFLAGASRTLAGKLLSLDWTDWSARTHALVR
                     HPCCPACGTEAPREAKPLPLEPGRATFNKDGGRRAVSPERT"
     gene            complement(1022..1306)
                     /locus_tag="P5201_13280"
     CDS             complement(1022..1306)
                     /locus_tag="P5201_13280"
                     /inference="COORDINATES: protein motif:HMM:TIGR03793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NHLP leader peptide family RiPP precursor"
                     /protein_id="HRV99563.1"
                     /translation="MDRKEMRKVLAKLIAKAWADARFKAKLMTDPASVLRAEGVAIPE
                     GVKIDVFENTDERIHLVIPKKPDELSDEALDIAAAGGQYSWDDNNCFITW"
BASE COUNT          235 a          509 c          455 g          262 t
ORIGIN      
        1 cgtccgttcg ggggagaccg cgcggcgccc cccgtccttg ttgaatgtag ctctccccgg
       61 ttccaacggc agcggcttcg cctcgcgggg cgcctccgtg ccgcaggcgg ggcaacaggg
      121 gtggcgcaca agggcgtggg ttcgagcgct ccagtcggtc cagtcgaggc tcagcagctt
      181 ccccgcaagc gtgcgggacg ctccggcgag gaagcgcgcc gcctccgtcg ccgccatgcc
      241 gtacgccgag gccagcgtcg acaggagagc cgctacggag gtcgagggaa ctgccttccc
      301 gcctccgagc cttttcgcca gagcgtgcgc gctgcggttg cgttcgagtc tcgacagaag
      361 acagcggaag caggcggtct ctccgggcag gaagagcggg ccgatccacg cctccggccc
      421 cgtgggacgg agcagcagcc aggggcgacg cgccgcgagc gcctccccgt tgatctcgcg
      481 cagctcgtcc cgctggtagt cgtccgcgac gacgacccac aagtccgccg actctttcgg
      541 ccccgtccgc acgcccgctt ctttcaacgc cgtctccagc ggcgaactgt ccagattccc
      601 cgcgacaaac acggcgacgg aactcctccg aagcgcctcc atcgcggcgc gcggttcgac
      661 gcccgcttcg tgccagaaag cggacagggc gggaggtatt tccggagttg cctccgcgac
      721 gcacccccgc tgctccagga tgttcaagac gtagtagaca gtcgctgcgt cgacgctgcc
      781 ggcgagcgcg tccgcgatgt cgtcggcgct gcggcatccg tcgatcagcg gaacaacaag
      841 ttcgtatgcg gcgccgtaaa aggctttcgc ccccctctcg gagagcgcga gcgctccttc
      901 gccggaaacc gtttgcaggc ggcagtgcgg cgcgaccgcc ggggtcttca tcgtgcgcct
      961 tccgaacaat acgggatgaa caatctcccg taccggatgg gagccgcgcc ggaagtttcc
     1021 gttaccacgt tatgaagcaa ttgttgtcat cccatgagta ctgcccgcct gcggcggcga
     1081 tgtcgagcgc ctcgtccgag agttcgtcgg gttttttcgg aatcacaagg tgtatgcgct
     1141 cgtccgtatt ttcgaagacg tcgattttta ccccttccgg aatcgccact ccctcggccc
     1201 tcaacaccga cgcgggatcg gtcatcagct tcgccttgaa ccgagcgtcc gcccacgcct
     1261 tggcgatcag cttcgccaat actttgcgca tctcttttct gtccataccg tacccctcct
     1321 gttgaaaagt agcgcttcgc tgtccggatt cgaaaaatag aagccccgtc ttcagagata
     1381 cggaagcggc ttgcccgaat ggcggcgctt cggcgcccgt ccgccgctca tgggctgacg
     1441 ggcgccgaag cgccgccatt c
//
LOCUS       DAOTIL010000790         1459 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminobacteriaceae bacterium isolate AMR_MDS_3026
            NODE_104523_length_1459_cov_4.749288, whole genome shotgun
            sequence.
ACCESSION   DAOTIL010000790 DAOTIL010000000
VERSION     DAOTIL010000790.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868050
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminobacteriaceae bacterium (wastewater metagenome)
  ORGANISM  Aminobacteriaceae bacterium
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae.
REFERENCE   1  (bases 1 to 1459)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1459)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:19:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,224
            CDSs (total)                      :: 3,198
            Genes (coding)                    :: 3,184
            CDSs (with protein)               :: 3,184
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1459
                     /organism="Aminobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_104523_length_1459_cov_4.749288"
                     /isolate="AMR_MDS_3026"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:3034609"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..841
                     /locus_tag="P5201_13285"
     CDS             <1..841
                     /locus_tag="P5201_13285"
                     /inference="COORDINATES: protein motif:HMM:NF012905.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="HAD-IC family P-type ATPase"
                     /protein_id="HRV99564.1"
                     /translation="GEAERGSNHPVARAVVEAAGALPPLGGAIEELPGRGVRLVGGGK
                     VILAGNARLLTDSGIASAPEAEGRPGAVVHVAEDGRYAGYIVVEDTVRSQSKRAVERL
                     RSLGVSTVGMLSGDRRENASRIAEDLGLDLWESDLLPSDKLEHFQRISERTGGTTLFV
                     GDGMNDAPLLAAADVGMAMGGLGADAAIEAADGVILNDDPLGAADVIEIARKTRSIVW
                     QNIAFALGVKGAVLTLGAFGMATMWEAVFADVGVALLATLNAARVLRFGERMQREAEE
                     IKV"
     gene            complement(954..>1459)
                     /locus_tag="P5201_13290"
     CDS             complement(954..>1459)
                     /locus_tag="P5201_13290"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HRV99565.1"
                     /translation="QLDQIGFAPVDCNLRTLIEENIALLQSRAKEKGVALSQETPPFL
                     SATLDRSMMGTVLRNLLNNALKFTAPGGRVLVSASQKGESVEIIVRDTGTGMSQEQLE
                     NLFRIDAKPVSTQGTSGEKGSGLGLILCKEFVEKNGGMIRAESVAGEGSSFFISLPSA
                     RGASDGA"
BASE COUNT          266 a          399 c          501 g          293 t
ORIGIN      
        1 gggggaggcg gagagagggt cgaatcaccc ggttgcgcgg gcagttgtag aggctgccgg
       61 agcgcttcct cctctcggcg gagcgatcga ggagctcccc ggcaggggcg ttcggcttgt
      121 cggcggggga aaggtaattc tcgcgggaaa cgctcgcctg ttgaccgact ccggcatcgc
      181 ctcggctccg gaggctgaag ggcgtccggg ggccgtggtg catgtcgctg aggacggccg
      241 atacgcaggg tacatcgtcg tcgaggatac ggtacgctcg cagtcgaaaa gagcggtgga
      301 gcgccttcgt tccctcgggg tgtctactgt cgggatgctc tcgggcgacc gtcgggagaa
      361 cgcctcccgc atagcggaag atctcggact ggatctctgg gagagcgatc ttcttccgtc
      421 cgataagctg gaacacttcc agcggatcag cgagcgaacg ggaggaacga cgctctttgt
      481 gggggatgga atgaacgacg ctccgctgct cgccgccgcg gatgtgggca tggccatggg
      541 cggcctggga gcggacgccg cgatcgaggc cgccgacggc gtcatcctga acgacgatcc
      601 gctcggcgcg gccgacgtca tcgaaatagc caggaagacg cggagcatcg tctggcagaa
      661 tattgccttt gcactcgggg tgaagggagc tgtactcacg ctgggagcgt tcggaatggc
      721 gaccatgtgg gaggcagtat tcgccgacgt gggagtcgcc cttcttgcaa cgctcaacgc
      781 agcgcgggtt ctgcgctttg gagagcgcat gcagcgcgaa gcagaggaaa ttaaagtatg
      841 aagaggtaga tgaatactga ccaaaagagt ggagaaaaga ggccgcagcg cgtcccgaac
      901 tgggggtttg ctgcggccgt tttgctgttg cgtgttgtgt aggtgtttta ttattacgct
      961 ccgtccgatg ctccgcgtgc ggatggcagc gaaatgaaaa aggagctccc ctctccggcg
     1021 acgctctccg cacgaatcat gcctccgttt ttttcgacga actccttgca gagaataaga
     1081 ccgagtccgc tccctttctc ccccgacgtc ccttgtgtgc tgacgggttt ggcgtcgatg
     1141 cggaagaggt tctctagctg ctcttgcgac atgccggttc cggtatcgcg tacgatgatc
     1201 tcgacgctct ctcccttctg ggatgcggaa acgaggacgc gccctccggg ggcggtgaac
     1261 ttcagcgcgt tgttcagcag attgcggagc accgttccca tcatgcttct atccagcgtg
     1321 gcggaaagga aaggcggcgt ctcttgagag agggcgactc ccttctcctt cgcacgggat
     1381 tgcagtagag cgatgttctc ctcgatcagc gtgcgcaggt tgcagtcgac gggggcgaat
     1441 ccaatctggt caagctgcg
//
LOCUS       DAOTIL010000791         1459 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminobacteriaceae bacterium isolate AMR_MDS_3026
            NODE_104543_length_1459_cov_3.363248, whole genome shotgun
            sequence.
ACCESSION   DAOTIL010000791 DAOTIL010000000
VERSION     DAOTIL010000791.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868050
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminobacteriaceae bacterium (wastewater metagenome)
  ORGANISM  Aminobacteriaceae bacterium
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae.
REFERENCE   1  (bases 1 to 1459)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1459)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:19:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,224
            CDSs (total)                      :: 3,198
            Genes (coding)                    :: 3,184
            CDSs (with protein)               :: 3,184
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1459
                     /organism="Aminobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_104543_length_1459_cov_3.363248"
                     /isolate="AMR_MDS_3026"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:3034609"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..366
                     /locus_tag="P5201_13295"
     CDS             <1..366
                     /locus_tag="P5201_13295"
                     /inference="COORDINATES: protein motif:HMM:NF014801.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FGGY-family carbohydrate kinase"
                     /protein_id="HRV99566.1"
                     /translation="VMEGITYGLRDSFELVKAATPVKRVLLVGGGAKNAAWRRMIAAN
                     VKASVSVPLVDEGGAYGAAMLAALGGGVSLDEVKGWIKIRETVEPDPAESAAYDRVYP
                     QFRALYADLKERFDATAGM"
     gene            479..>1459
                     /locus_tag="P5201_13300"
     CDS             479..>1459
                     /locus_tag="P5201_13300"
                     /inference="COORDINATES: protein motif:HMM:NF012488.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HRV99567.1"
                     /translation="MKQYAIGLVPGPVSVPEEFRRAYLENFGSADLEEEFFLLYEENE
                     SLLRKLLKTKNAVTIQSGEAMSVLWGALKSILLPGDRLLAVSNGVFGRGFGEMGMMLG
                     MEVRIVEAPDGEFPDLTTIRTAAAEFRPRMISAVHCETPSGILNPVAGVGEIARETGA
                     LFCVDYVASAGGADVRTDEWGIDIGLLGSQKALSLLPDLSMVAVSDQAWEAAERTNYQ
                     GYDALLPWRDGPKNRYLPYTHNWHALYALSLSLRKLFEEGLDRSFARHEEVARYCRKR
                     LSAMNVALYPKSEALCSPTVTAALVPQGWTWSELNGALRNRGMAVGGSYGP"
BASE COUNT          275 a          440 c          500 g          244 t
ORIGIN      
        1 gtgatggagg ggatcaccta cggcctgcgc gactcctttg agctggtgaa ggcggccacc
       61 cctgtgaagc gggtgctcct cgtcggcggc ggcgcgaaga acgccgcgtg gcgccgcatg
      121 atcgccgcca acgtcaaggc gtccgtctcg gttccgctgg tggacgaagg aggggcctac
      181 ggcgcggcga tgctcgcggc gctcggcggc ggcgtttccc tggacgaggt gaaggggtgg
      241 atcaaaatcc gcgaaaccgt cgaaccggac ccggcggagt ccgcggcgta cgacagggtg
      301 tacccgcaat ttcgggcgct ctacgccgat ctgaaggagc gtttcgacgc cacggcgggc
      361 atgtagccgt cttggcttga attctcgcag gaaaacagaa acacggagga ggctcccggg
      421 cctcctcttt tcttcacgga acacacactc aatcagtcgg aaacggagga tgcaacgcat
      481 gaaacagtac gcaatcggcc tcgtaccggg gcccgtatcg gttcccgagg agtttcggag
      541 agcctacctg gagaacttcg gcagcgccga cctcgaagag gagttcttcc tcctctacga
      601 ggagaacgag tcgctgctcc gaaaacttct gaagacgaaa aacgccgtga ccatccagag
      661 cggagaggcg atgtccgtgc tctggggcgc tctgaagagc atcctgctgc ccggcgaccg
      721 gctgcttgcc gtctcgaacg gggttttcgg caggggattc ggagagatgg ggatgatgct
      781 cggcatggaa gtgcgcatcg tcgaggctcc ggatggggaa ttcccggatc tcacaacgat
      841 acggacggcg gcggccgaat tccgtcccag gatgatctcc gccgtccact gcgaaacgcc
      901 gagcggtatt ctgaacccgg tggcgggcgt cggcgaaata gcgagggaga ccggcgccct
      961 cttctgcgtc gactacgtgg cgagcgcggg aggcgcggac gtccgcaccg atgaatgggg
     1021 aatcgacatt gggcttctcg ggagccagaa ggcgctctcg ctgctgcccg atctttcgat
     1081 ggtcgcggtg agcgatcagg catgggaggc tgcggagcgg acgaactatc aggggtacga
     1141 cgcgctgctt ccgtggagag acggtccgaa aaaccgctac cttccctaca cccacaactg
     1201 gcacgcgctg tacgcgctct cgctctcgct ccggaagcta ttcgaggagg ggctggatcg
     1261 ctccttcgcc cggcatgaag aggttgcccg gtactgccgc aagcgccttt ccgcgatgaa
     1321 cgtcgcgctc tacccgaaga gcgaggcgct ctgctccccc accgtcaccg cggcgctcgt
     1381 gccgcagggg tggacgtgga gcgaactcaa cggcgcactc aggaacagag ggatggcggt
     1441 cggcggcagc tacggaccg
//
LOCUS       DAOTIL010000792         1455 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminobacteriaceae bacterium isolate AMR_MDS_3026
            NODE_104991_length_1455_cov_3.221429, whole genome shotgun
            sequence.
ACCESSION   DAOTIL010000792 DAOTIL010000000
VERSION     DAOTIL010000792.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868050
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminobacteriaceae bacterium (wastewater metagenome)
  ORGANISM  Aminobacteriaceae bacterium
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae.
REFERENCE   1  (bases 1 to 1455)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1455)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:19:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,224
            CDSs (total)                      :: 3,198
            Genes (coding)                    :: 3,184
            CDSs (with protein)               :: 3,184
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1455
                     /organism="Aminobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_104991_length_1455_cov_3.221429"
                     /isolate="AMR_MDS_3026"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:3034609"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..476
                     /locus_tag="P5201_13305"
     CDS             <1..476
                     /locus_tag="P5201_13305"
                     /inference="COORDINATES: protein motif:HMM:NF019498.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HRV99568.1"
                     /translation="AGAPKRIVHAGTRKLRRLFKKRKRGGRMSGMNVFEKFNTGKMRI
                     PGGEREFDSIEWKKHPAFNGVELKHLLTGEQTGGSFSFHLVRIAPGSAIGLHVHETQL
                     ETHEVIAGQGVCLNDGTEVPYDAGTVSFFPAGVPHEVRAGTEGLSLFAKFFPALC"
     gene            complement(387..1229)
                     /locus_tag="P5201_13310"
     CDS             complement(387..1229)
                     /locus_tag="P5201_13310"
                     /inference="COORDINATES: protein motif:HMM:NF025878.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HRV99569.1"
                     /translation="MKEKEVRYYFAEQRGLEVVRLKGSRFFYPEHSHVSTYTASLVLS
                     GALRLEAGSATTVHGPDSFFVIPPYEPHALSSSGGCETVTICMGDDLLRALSGGEKSL
                     NDCLRALSGALPAAPWIFTERFRRMLDRLFSSVREPGRRERDFVGAVEQRLLLHPEEP
                     LDLETFAEALHVSRFHLLREFKRSVGLPPHKFQMQNRIRKARKMMLDTRLPLTEVALA
                     AGFYDQSHFIRCFRKLAGMTPSEFREACAIGIPSAERGKKLGEEREPLRPRADFVRNP
                     CREE"
BASE COUNT          314 a          432 c          456 g          253 t
ORIGIN      
        1 ccgcgggcgc cccgaagaga atagtgcatg ccgggacgag aaaactccgg cgactcttca
       61 agaaaaggaa aaggggcggt cgtatgagcg gcatgaacgt gttcgaaaaa ttcaatactg
      121 gcaagatgcg cataccgggc ggagagaggg agttcgactc catcgagtgg aagaaacatc
      181 cggcgttcaa cggcgtggag ctcaagcatc tgctgacggg ggagcagacg ggagggagtt
      241 tcagctttca tctcgtgcgc atcgccccgg gaagcgcgat cggactgcat gtccacgaga
      301 cgcagcttga gacgcacgag gtgatcgcag gacaaggagt ctgcctgaac gacgggacgg
      361 aggtccccta cgatgcaggg acggtctcat tcttcccggc aggggttccg cacgaagtcc
      421 gcgcggggac ggaggggctc tcgctcttcg ccaagttttt tcccgcgctc tgctgaaggt
      481 attccgatag cgcacgcctc ccggaactcc gagggggtca ttccggcgag cttccggaag
      541 catctgatga agtggctctg gtcgtagaat ccggcggcca gggccacttc cgtcagaggg
      601 aggcgcgtat cgagcatcat cttccgcgct tttcgaatcc tgttctgcat ctggaacttg
      661 tgcggaggga ggccgacgct tcttttgaac tctctgagca gatggaacct gctgacgtgc
      721 agggcttccg cgaaggtttc gagatcaagg ggctcctcag gatgaaggag cagccgctgc
      781 tccacagcac cgacgaaatc acgctccctg cggccaggct cccgcaccga agaaaaaaga
      841 cggtcgagca tccgccggaa cctttcggta aaaatccagg gagcggctgg gagcgcgccg
      901 gaaagagcgc gcaggcagtc gttcagggat ttttccccgc cggagagagc ccgcaacaga
      961 tcgtcgccca tgcagatggt gaccgtttcg cagccgccgg aggaggagag ggcgtgcggt
     1021 tcgtaggggg gaatcacgaa aaaagagtcg ggcccgtgga cggtcgttgc gctccccgcc
     1081 tcaagacgca gcgcccccga cagaaccagg ctcgccgtat aggtggacac atgactgtgt
     1141 tccgggtaga agaagcgcga tcccttcagg cgcacaactt ccagcccccg ctgttccgcg
     1201 aaatagtagc ggacctcttt ctccttcatc gcgaatcccc ttttcgaaca cgcgcgtcaa
     1261 gtatatttca taggtacccg acgaacaacc ttatgagcgc cgtctgcgct ttccgcatcc
     1321 gttgacgacg aggggaacaa agagcagcag cgcggccgtc cacgcattcc ccacaccgac
     1381 cgagcatccg cctccgccgc cgcccccttg ctgctgcacc gctgcgatga agactttgac
     1441 gagagcaagc gcagc
//
LOCUS       DAOTIL010000793         1448 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminobacteriaceae bacterium isolate AMR_MDS_3026
            NODE_105685_length_1448_cov_2.884422, whole genome shotgun
            sequence.
ACCESSION   DAOTIL010000793 DAOTIL010000000
VERSION     DAOTIL010000793.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868050
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminobacteriaceae bacterium (wastewater metagenome)
  ORGANISM  Aminobacteriaceae bacterium
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae.
REFERENCE   1  (bases 1 to 1448)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1448)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:19:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,224
            CDSs (total)                      :: 3,198
            Genes (coding)                    :: 3,184
            CDSs (with protein)               :: 3,184
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1448
                     /organism="Aminobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_105685_length_1448_cov_2.884422"
                     /isolate="AMR_MDS_3026"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:3034609"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            237..869
                     /locus_tag="P5201_13315"
     CDS             237..869
                     /locus_tag="P5201_13315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012869276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit D"
                     /protein_id="HRV99570.1"
                     /translation="MTQKIAPTRGNMVKFASSLKLARKGRDLLEQKRTILLMELTGKI
                     REARDLQRDLELVFSDAYFSLQMAGLSVGIDNVEELAYSVPEVQDFTIRLHSVMGVEI
                     PKVDPMEKPPVPCYSFLGTSGALDAAYSRAQDVLALIARLAEVETSVYRLAVQIRKTF
                     RRVNALEKVVIPSNKRTLAWIANVLEENDREDFTRMKMARKNTSEGGGAR"
     gene            866..1201
                     /locus_tag="P5201_13320"
     CDS             866..1201
                     /locus_tag="P5201_13320"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRV99571.1"
                     /translation="MNLSAVLGELLASEDEAVNGVETAKRKASKILREAREHFAADQE
                     ARLAGVRAQSKTIVESALHSAELEAAQIAEMGANGRRKMEEHFAESAPEIVRSLVMET
                     AARYGRLGG"
     gene            1227..>1448
                     /locus_tag="P5201_13325"
     CDS             1227..>1448
                     /locus_tag="P5201_13325"
                     /inference="COORDINATES: protein motif:HMM:NF014094.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATPase subunit"
                     /protein_id="HRV99572.1"
                     /translation="MSFLSAGERAAVTGKARVFRGRLLAPDDYLHLLKCGTVGEVIMY
                     LGRTPSYSSYFHGEYSEAIHRGEIEGILSI"
BASE COUNT          300 a          402 c          495 g          251 t
ORIGIN      
        1 tcgccagcgt tcgaaaaagt acgttgcata caagcacctc caaagcactg cgtcattgag
       61 aagatcgtat agagtcgccc ggcggaccgc agagcaaaaa aggccgctcc attccgttgc
      121 agcgactgta taaaagtata ccacgcaatc ggaaacgaaa agcctctacc ttctatagtt
      181 gactttttta acccggagtt ataaagtgct ttttatgtga acgggaggtg cggcaggtga
      241 cccagaagat cgcccccacg cggggcaaca tggtgaagtt cgcgtcgtcg ctgaagctgg
      301 cccggaaagg gcgcgacctc ctggagcaga agcggacgat cctgctgatg gagctgacgg
      361 ggaagatacg cgaggcgcgg gatctgcagc gcgacctcga acttgtcttt tccgacgcct
      421 atttcagcct gcagatggcc gggctctccg tggggatcga caacgtggag gagcttgcct
      481 actccgtccc cgaggtgcag gacttcacca tccgcctgca ctccgtgatg ggagtggaga
      541 tcccgaaggt ggacccgatg gagaagccgc ccgttccctg ctactccttt cttggaacgt
      601 cgggagcgct cgacgccgcc tacagccggg cgcaggacgt gcttgcgctc atcgcgcggc
      661 tggcggaggt ggagacgtcg gtctaccggc tggcggtgca gatccgcaag acgttccgga
      721 gggtgaacgc gctggagaag gtcgtcattc cgtcgaacaa gaggaccttg gcgtggatcg
      781 ccaacgtgct tgaggagaac gaccgggagg acttcacgcg catgaagatg gcgcgaaaga
      841 atacttcgga agggggaggt gcgcgatgaa tctgagcgct gtgctgggcg agcttctcgc
      901 ctcggaggac gaggccgtca acggagtgga gacggcgaag cggaaggcgt ccaagattct
      961 ccgcgaggcg cgggagcatt tcgccgcgga ccaggaggcg aggctggccg gagtccgggc
     1021 gcagtcgaag acgatcgtgg agtccgccct gcactcggcg gagcttgagg cggcgcagat
     1081 cgccgagatg ggagccaacg ggcggcggaa gatggaggaa catttcgccg agtccgcccc
     1141 ggagatcgtg cggagcctcg tcatggagac cgccgcgcgc tacgggagac tgggaggatg
     1201 accgaaggcg cgggggagga gtgcgcatgt cctttctttc ggcgggcgaa cgggctgcgg
     1261 taacaggaaa ggcgcgcgtc ttccggggac ggctgctcgc ccccgacgac tatctgcatc
     1321 tcctgaagtg cggaacggtt ggggaagtca tcatgtatct ggggcgcacc ccctcctact
     1381 cctcgtactt ccatggagag tactccgaag ccattcaccg cggggaaatc gaaggaatcc
     1441 tctcgatc
//
LOCUS       DAOTIL010000794         1445 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminobacteriaceae bacterium isolate AMR_MDS_3026
            NODE_105974_length_1445_cov_3.304317, whole genome shotgun
            sequence.
ACCESSION   DAOTIL010000794 DAOTIL010000000
VERSION     DAOTIL010000794.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868050
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminobacteriaceae bacterium (wastewater metagenome)
  ORGANISM  Aminobacteriaceae bacterium
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae.
REFERENCE   1  (bases 1 to 1445)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1445)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:19:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,224
            CDSs (total)                      :: 3,198
            Genes (coding)                    :: 3,184
            CDSs (with protein)               :: 3,184
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1445
                     /organism="Aminobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_105974_length_1445_cov_3.304317"
                     /isolate="AMR_MDS_3026"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:3034609"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..575
                     /locus_tag="P5201_13330"
     CDS             <1..575
                     /locus_tag="P5201_13330"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRV99573.1"
                     /translation="KDLGETREKFEKEYAPILDGKLFSDVPFFRNRPYKDLAKPVIEL
                     EVRIKTAIVSTVQKEKSAEELQKAIELDEELTRKEIQRIQELQSMISREKWYALQLED
                     VRAAGGTTRVRELQIVMARHPEGSEEYRKASEEAHLIQDEQHPEQLAVKKKLREFEEL
                     ERKVFEEIRKLHEEHEKRRKSLGLAPGLSK"
     gene            613..1287
                     /locus_tag="P5201_13335"
     CDS             613..1287
                     /locus_tag="P5201_13335"
                     /inference="COORDINATES: protein motif:HMM:NF015945.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PspA/IM30 family protein"
                     /protein_id="HRV99574.1"
                     /translation="MSIFARVSDIFKANVNEILDRMEDPEKMVKQMIIEMEEALVKAT
                     SALAKAMANERNLRKQQENALSQAKQWEEKAAMALKAGNADLAKQALSKKIIADGQAK
                     QYDAMVAQASSVTSQLRSQLDTLKTRLDEARMKQATLVARAQTAKTQKEFSTVMGSNV
                     GQGAFAKFEKMEKKIEGMEAEAQAFSELSGDAPADDPFKDMEKDMQLEAEMAKLMEKM
                     GGGAGA"
BASE COUNT          376 a          333 c          495 g          241 t
ORIGIN      
        1 ggaaggatct gggggagaca cgcgagaagt ttgaaaagga gtacgccccg attctggacg
       61 ggaagctctt ttccgatgtc ccgtttttcc ggaacaggcc gtacaaggat ctcgcgaaac
      121 ccgtgattga acttgaggta cggatcaaaa cggcgattgt ctcaactgtg cagaaggaaa
      181 agagcgccga agagttgcaa aaggcgatcg agctcgatga ggagctcacg aggaaggaga
      241 ttcagcgtat tcaggagttg cagagtatga tctcccggga aaagtggtat gcgctgcagc
      301 tcgaggacgt cagagccgcc gggggaacga cccgggttcg cgaactccag attgtcatgg
      361 cgcggcatcc ggaaggctct gaagagtatc gcaaggcttc cgaggaggct cacttgattc
      421 aagacgaaca gcaccccgag cagctggcgg tcaagaagaa gttgcgggag ttcgaagagc
      481 ttgaacgcaa ggttttcgaa gaaatacgaa agctgcatga ggagcacgaa aagcggcgga
      541 agagtctcgg tcttgctccg gggctgagca agtaaaacag gctgccatta aaaacacaca
      601 aggaggggga ttatgagcat attcgcgcgg gtatcggata tcttcaaggc gaacgtcaac
      661 gaaatactgg acaggatgga agatccggag aagatggtca aacagatgat catcgagatg
      721 gaggaggcgc ttgtaaaggc tacgtcggcg ttggcgaagg cgatggccaa cgagcgcaac
      781 ctccgcaagc agcaggagaa cgcgctctct caggcgaaac agtgggagga gaaggctgcg
      841 atggccctca aggcggggaa cgccgatctt gcgaagcagg cgctctccaa gaagatcatt
      901 gccgacggac aggcaaagca gtacgacgca atggtggcgc aggcttccag cgtcacgtcc
      961 cagcttcgtt cacagctcga cacgctcaag acgcggctcg acgaagcaag gatgaagcag
     1021 gcgactttag tcgcccgcgc tcagacggcg aagacgcaga aggagttttc cacggtcatg
     1081 gggagtaacg tcggccaggg cgccttcgcg aagttcgaga aaatggagaa gaagatcgag
     1141 gggatggagg cggaggcgca ggcgttctcg gaactctccg gcgacgcccc tgcggacgat
     1201 ccgttcaagg acatggagaa ggatatgcag cttgaggcgg agatggcgaa gctgatggag
     1261 aagatggggg gtggagcggg tgcataagtt tcacgtactc aaggacggcg tgaacggacc
     1321 aatgctggag gaggtccggg cgaaggtgaa tacctggctg cgggagttct tcgacgagga
     1381 cgatcttgtg cgggtggagg acctcgtctc cttctccttt ggcagcgcga cgatccaggt
     1441 gacgg
//
LOCUS       DAOTIL010000795         1442 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminobacteriaceae bacterium isolate AMR_MDS_3026
            NODE_106264_length_1442_cov_4.288392, whole genome shotgun
            sequence.
ACCESSION   DAOTIL010000795 DAOTIL010000000
VERSION     DAOTIL010000795.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868050
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminobacteriaceae bacterium (wastewater metagenome)
  ORGANISM  Aminobacteriaceae bacterium
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae.
REFERENCE   1  (bases 1 to 1442)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1442)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:19:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,224
            CDSs (total)                      :: 3,198
            Genes (coding)                    :: 3,184
            CDSs (with protein)               :: 3,184
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1442
                     /organism="Aminobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_106264_length_1442_cov_4.288392"
                     /isolate="AMR_MDS_3026"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:3034609"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..509)
                     /locus_tag="P5201_13340"
     CDS             complement(<1..509)
                     /locus_tag="P5201_13340"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HRV99575.1"
                     /translation="MNDEKAIVVRGLTFSYRGDEDVRAVKSLDFEVDRGEFVVIMGPS
                     GAGKSTLANSLNGLIPRFLRGKYEGSVLVNGLDAAKHSVSRMADEIGLVFQDFEAQLF
                     STNVKLEVAFGPENFAVPREEIAERIGRVLKTVNLEGVEGRSPATLSGGQKQRLAIGS
                     VLAMHPDIV"
     gene            777..>1442
                     /locus_tag="P5201_13345"
     CDS             777..>1442
                     /locus_tag="P5201_13345"
                     /inference="COORDINATES: protein motif:HMM:NF013088.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HRV99576.1"
                     /translation="MYLAGVLYCCAAAVCWALGPVFLKKGLALMSHSEMGAARTFGFV
                     GAALFFVMLEPGVAVGWNYPLPYLAVIFVSILIGNIVGDLAYFRSIEMIGVGRAVGTT
                     SCYPLFVTAISSVWLGEAVTLPLVLGTLVMIAGLVLLKSGGKQSAETRSVSGAGFFFA
                     IVAAVSWAGMMVLQKWLISVHSVHPASITLWRSFFLFSLSWLYWGTSSLRRGTNPMRI
                     FAMS"
BASE COUNT          249 a          463 c          392 g          338 t
ORIGIN      
        1 cagacgatat cggggtgcat cgcgaggacc gagccgatgg cgaggcgctg cttctgcccg
       61 ccggagagcg tcgcggggct gcgcccctcc actccttcaa gattcacagt cttcagcacc
      121 ctgccgatgc gctccgcgat ttcctcccgg gggaccgcga aattctccgg gccgaacgcg
      181 acttcgagct ttacgttggt cgaaaagagc tgcgcctcga agtcctgaaa cacaagacct
      241 atctcgtcgg ccatccggct gacggagtgt ttcgccgcat ccagaccgtt tacgagcacg
      301 cttccctcgt acttcccgcg cagaaaacgg gggatcagcc cgttcaggct gttcgccaga
      361 gtcgacttcc ccgcgccgct cggtcccatg atcacgacga actcgcccct gtccacttcg
      421 aaatcgagcg acttcaccgc ccggacgtcc tcgtcccctc tgtaggaaaa agtaagccct
      481 cgtaccacta tggctttttc atcattcatt acgtaaatcc ccgccccttt cggtgggtag
      541 cgctgttgga atgcgcacat cggccgcgtt tccttcgcag gagacacgaa tgcgccaccc
      601 cggattatac cagagggggc cggctctttc cctgaaaaag cgaaggaaac cggctccctt
      661 tttcacagag gacccggtct ccactctctt tcgcacgaaa agaagtttct gtgctatcat
      721 tgcgtgcaat gtcacatcga tattccgtgg tccgccggac cgaggaggct actcaagtgt
      781 atcttgcagg tgttttgtat tgctgcgccg cagcagtgtg ctgggctctc gggcctgttt
      841 ttctgaagaa agggctcgcg ttgatgagcc attcggaaat gggagcggcg cggaccttcg
      901 gcttcgtcgg cgcggcgctg tttttcgtca tgctggaacc tggcgtggca gtcgggtgga
      961 actatcccct tccctatctt gccgtcatct tcgtcagcat cctcatcggc aacatcgtcg
     1021 gcgacctcgc ctacttccgc tcgatcgaga tgatcggcgt cggcagagct gtgggaacca
     1081 cttcctgcta ccccctcttc gtcaccgcga tctcttcggt ctggctcggc gaagccgtta
     1141 cgcttcctct cgtacttgga acgctcgtga tgatcgccgg gctcgttttg ctgaaatcgg
     1201 gggggaaaca gtctgcggaa acgcgctcag tctccggagc gggcttcttt ttcgccattg
     1261 tcgcggcagt atcctgggcg gggatgatgg tactccagaa gtggctcata tcggtccact
     1321 ccgtccaccc tgcctccatc accctctggc gctccttctt tctcttctct ctctcatggc
     1381 tctattgggg aacaagttcc ctgcgccggg gaacaaaccc gatgcggatc ttcgccatga
     1441 gc
//
LOCUS       DAOTIL010000796         1440 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminobacteriaceae bacterium isolate AMR_MDS_3026
            NODE_106482_length_1440_cov_4.469314, whole genome shotgun
            sequence.
ACCESSION   DAOTIL010000796 DAOTIL010000000
VERSION     DAOTIL010000796.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868050
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminobacteriaceae bacterium (wastewater metagenome)
  ORGANISM  Aminobacteriaceae bacterium
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae.
REFERENCE   1  (bases 1 to 1440)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1440)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:19:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,224
            CDSs (total)                      :: 3,198
            Genes (coding)                    :: 3,184
            CDSs (with protein)               :: 3,184
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1440
                     /organism="Aminobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_106482_length_1440_cov_4.469314"
                     /isolate="AMR_MDS_3026"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:3034609"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..727)
                     /locus_tag="P5201_13350"
     CDS             complement(<1..727)
                     /locus_tag="P5201_13350"
                     /inference="COORDINATES: protein motif:HMM:NF013231.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family winged helix-turn-helix
                     transcriptional regulator"
                     /protein_id="HRV99577.1"
                     /translation="MKEKPHEITQKIRFFNRFYTRIIGAVNQSVLNSPFSMAEVRVLF
                     EIKHSDNPTASEVNRELELDPGYLSRIVRRFEQDELLTKERSHLDGRAYLLKLTEHGE
                     RVLASLEKASDKKAADLISPLSEREITALLRSMETIHSLLTKTPLELEIRPAAPGELG
                     TIVARHMDYYSKQYAFDASFESYLFEGIARFLKNRENGMGQAWVVKHGGVVVGSIAID
                     HVEDRVAQLRWFLVEPEYRGMGMG"
     gene            complement(877..>1440)
                     /locus_tag="P5201_13355"
     CDS             complement(877..>1440)
                     /locus_tag="P5201_13355"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="B12-binding domain-containing radical SAM
                     protein"
                     /protein_id="HRV99578.1"
                     /translation="FYDDALLIDKDRRFYPLCEELVRRFPGVRYHTPNGLHIREIDER
                     CAAALKQTGFQTMRLSLESSDPAFQRKGSGKTSGEEFQHALRNLQKAGFAPEQMETYI
                     LAGLPGQTTVSVEASIRFVDSCGARAKLAEFSPVPGTPSFEEAASILPELRSEPLLHN
                     KTVYSSFLSGNISPEKLQFLKDLTKHR"
BASE COUNT          292 a          379 c          407 g          362 t
ORIGIN      
        1 tccccattcc catcccgcgg tactccggtt ctacgaggaa ccagcggagc tgggcaaccc
       61 gatcttcgac atggtctatc gcgatggagc cgacaaccac accgccgtgc ttcacaaccc
      121 aggcctgccc catgccgttc tccctgttct tcaggaaacg ggctattcct tcgaagaggt
      181 aggactcaaa cgacgcgtcg aatgcgtact gtttgctgta atagtccatg tgacgcgcaa
      241 cgatcgtgcc gagttcgccc ggagctgcag gccggatttc gagctcaagc ggagttttcg
      301 ttaaaagtga gtggatggtc tccatagagc gcagcaacgc tgtgatttct ctctctgaaa
      361 ggggggagat aagatccgcc gctttcttgt ccgacgcctt ttcgagagag gcgagcacac
      421 gttcgccatg ttcggtcagc ttgagcagat acgctcttcc atcgagatgc gaacgctcct
      481 tcgtcagcag ttcatcctgt tcaaagcggc ggactatcct gctcaagtac cccgggtcga
      541 gttcgagctc cctgttgact tcgcttgccg tagggttatc ggagtgtttt atctcgaaga
      601 gcactctcac ctccgccatg gagaatggac tgttcaacac cgactggttg acggcgccga
      661 tgatccgtgt ataaaacctg ttgaaaaaac gtatcttctg tgtgatttcg tgcggcttct
      721 ctttcacttc catcacatcc tttcgttttg gattttcact ctgtaatgat atttcttatc
      781 attgatgctg tcaagcaaat tatcgcccgt ttggaagaaa ttttggtgga aatacaaccc
      841 tttcggtgag gagctcgaga tgtgtatgca catatatcat cgatgcttcg ttaaatcctt
      901 taggaattga agtttttcgg gagagatatt ccctgagagg aatgacgagt agaccgtctt
      961 gttgtgcagc agcggctcgc tccgaagttc agggagaatg gaggcggctt cctcgaacga
     1021 gggcgttccc ggcacagggg agaattccgc aagctttgcc ctcgcgccgc atgagtcgac
     1081 gaagcgtatc gacgcttcta cggagacggt cgtctgcccg ggaaggccgg cgagaatgta
     1141 cgtttccatc tgttcgggtg cgaagcccgc cttttgaagg ttgcgaaggg cgtgttggaa
     1201 ctcttctccg gacgtcttgc cggagccctt tcgttgaaag gcggggtcgc tgctctcaag
     1261 gctcaggcgc atggtttgga atcccgtctg tttgagtgcg gcggcgcagc gttcgtcgat
     1321 ctccctgatg tggagcccgt tcggcgtatg gtagcgaacg ccggggaaac gacggacgag
     1381 ttcctcgcag agagggtaaa agcgccgatc cttgtcgatc agcagcgcgt cgtcgtagaa
//
LOCUS       DAOTIL010000797         1440 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminobacteriaceae bacterium isolate AMR_MDS_3026
            NODE_106535_length_1440_cov_2.293141, whole genome shotgun
            sequence.
ACCESSION   DAOTIL010000797 DAOTIL010000000
VERSION     DAOTIL010000797.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868050
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminobacteriaceae bacterium (wastewater metagenome)
  ORGANISM  Aminobacteriaceae bacterium
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae.
REFERENCE   1  (bases 1 to 1440)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1440)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:19:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,224
            CDSs (total)                      :: 3,198
            Genes (coding)                    :: 3,184
            CDSs (with protein)               :: 3,184
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1440
                     /organism="Aminobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_106535_length_1440_cov_2.293141"
                     /isolate="AMR_MDS_3026"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:3034609"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..529
                     /gene="panF"
                     /locus_tag="P5201_13360"
     CDS             <1..529
                     /gene="panF"
                     /locus_tag="P5201_13360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008521144.1"
                     /note="mediates high affinitiy panthothenate transport
                     which is stimulated by the presence of sodium ions; member
                     of SSS family of sodium/solute symporters; the imported
                     panthothenate is phosphorylated by panthothenate kinase;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="sodium/panthothenate symporter"
                     /protein_id="HRV99579.1"
                     /translation="LITNLFNPVVAGIILAGPLAAVMSTVDSQLLVVVAAIVNDLLVN
                     YIKPELKANFKKLRAITIATCFVVGAIIVALAFDPPELMVWLNLFATAGQLSTFLWPT
                     ILGLYWKGANAPGAIAGMVSGIGTYLYFNYYLPRPLGLHPIVPSLALSLIAFIVVAKM
                     TKKPSEKVIRLFWEL"
     gene            719..1249
                     /locus_tag="P5201_13365"
     CDS             719..1249
                     /locus_tag="P5201_13365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005660992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FmdE family protein"
                     /protein_id="HRV99580.1"
                     /translation="MEQNMHLPEDLKPVIDFHGHFCPGVLIGWRASKLALKLLGVGRD
                     RDEELVAITENDACGVDAVQYVLGCTFGKGNLVFRDYGKQAFTVFRRSDGKGVRLVLK
                     SPGRELTREESARRLLEESDEALFTVGEPKEPMPERAVIRTSACCDACGERAMETRIR
                     TMEDGRRLCVPCAELG"
BASE COUNT          291 a          433 c          414 g          302 t
ORIGIN      
        1 gttgatcacc aacctcttca acccggtcgt cgccggaatc atcctggccg gtcctctcgc
       61 ggccgtcatg tcgaccgtgg attcgcagct cctcgtggtg gtggcggcca tcgtcaacga
      121 cctgctcgtc aactacatca aaccagaatt gaaggcaaat ttcaaaaaac ttcgcgccat
      181 caccatcgcc acctgcttcg tcgtcggcgc gatcatcgtg gcgctggcct tcgacccccc
      241 ggagctcatg gtgtggctga acttgttcgc gacagcgggg cagctttcga ccttcctgtg
      301 gcccaccatt ctggggttgt actggaaggg cgccaacgcc cccggcgcga tcgcggggat
      361 ggtctcgggc atcggaacct acctctattt caactactac cttccgaggc cccttggact
      421 gcaccccatc gttccctcgc tggcgctttc tctgatcgcg ttcatcgtcg tcgccaagat
      481 gacgaaaaaa ccgtccgaaa aggtcatccg gctcttctgg gagctgtaag gcgaaacgta
      541 cgagcgggag acgcggcgac gcagcctccc gcccgtattt gttgcaaggg acttcttttt
      601 tcttctcctc cataattcac agcctcgtcc gttctctttt gccgagacgc atgctacaat
      661 agcacgaaat tataatttcg tgctatgatt cttcgcccgt acacagagga ggctgccaat
      721 ggaacagaat atgcatctcc ccgaggactt gaagcccgtg atcgactttc acggccactt
      781 ctgccccggc gtgctcatag gatggagggc gtcgaaactc gccctgaaac tgctcggcgt
      841 cgggcgcgac cgcgatgaag agcttgtcgc gattaccgag aacgacgcgt gcggcgtgga
      901 cgcggtgcag tatgtattgg gctgcacctt cggcaaggga aacctcgtct tccgcgacta
      961 cggcaagcag gcgttcaccg tcttccgccg ctccgacggc aagggcgtcc gcctcgtgct
     1021 caaatccccc ggacgcgaac tgacccgcga ggagtcagcc cgccgtctcc tcgaagagag
     1081 cgacgaggcc ctcttcacgg tcggcgaacc gaaggagccg atgccggagc gcgcggtgat
     1141 ccgcacgtcg gcctgctgcg acgcctgcgg cgagcgcgcg atggagacgc ggatacgtac
     1201 aatggaggac ggcagacgcc tgtgcgtgcc gtgcgcggag ctcggataag cgtactttcc
     1261 gtcgcatcgt cgaatgtcgg agtattggaa gaaaaggttt tcccgcgaag aaggaggaag
     1321 cattcgcggg aaaggatgaa agcggagaaa acagctcgaa aaaaaaggga aatcagcggt
     1381 ccgaaggttt tttttcgctg tttccttcgg tttcgtagtg ttttttccag aaaaaattga
//
LOCUS       DAOTIL010000798         1437 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminobacteriaceae bacterium isolate AMR_MDS_3026
            NODE_106797_length_1437_cov_4.199711, whole genome shotgun
            sequence.
ACCESSION   DAOTIL010000798 DAOTIL010000000
VERSION     DAOTIL010000798.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868050
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminobacteriaceae bacterium (wastewater metagenome)
  ORGANISM  Aminobacteriaceae bacterium
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae.
REFERENCE   1  (bases 1 to 1437)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1437)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:19:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,224
            CDSs (total)                      :: 3,198
            Genes (coding)                    :: 3,184
            CDSs (with protein)               :: 3,184
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1437
                     /organism="Aminobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_106797_length_1437_cov_4.199711"
                     /isolate="AMR_MDS_3026"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:3034609"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..571)
                     /locus_tag="P5201_13370"
     CDS             complement(<1..571)
                     /locus_tag="P5201_13370"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRV99581.1"
                     /translation="MKKMLAALLVLLLVVSVASTALAYALDKGTAFENSVMLGKGNGG
                     GNGGGGGGNGGSSGGASGEKPCPWRVVVADDFAFTVYHPITGEPQHSTTGRWLGRWCD
                     GQGGIFGFFLRPEGDLVDPYQLALDALASVRIAPPDIRTSPSERGRLYVQVPTWLWID
                     RSWWQPYEATANAGRVSSTVRATPVATNWR"
     gene            complement(786..1214)
                     /locus_tag="P5201_13375"
     CDS             complement(786..1214)
                     /locus_tag="P5201_13375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005660084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicB family
                     antitoxin"
                     /protein_id="HRV99582.1"
                     /translation="MANRGNKDMRTYPAIFSKDKNGIAVEFPDLPGCLTCGKSEAEAV
                     ARAKEALEGFLYVSERDGDHIPAPTSFDEINVKKGEAVVLVTVRMDIVREEEAKRSIT
                     KSVTLPAWLNKLGMEHNLNFSSLLQDAIKERLDVKEPVSF"
     gene            complement(1243..>1437)
                     /locus_tag="P5201_13380"
     CDS             complement(1243..>1437)
                     /locus_tag="P5201_13380"
                     /inference="COORDINATES: protein motif:HMM:NF019540.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003729 - mRNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicA family
                     toxin"
                     /protein_id="HRV99583.1"
                     /translation="PIKSYSSREIIKMLEEDGWYFTGARDDHHYFEHDTKKGKITVPH
                     PEKDIHPKTSISILKQAGLK"
BASE COUNT          267 a          437 c          401 g          332 t
ORIGIN      
        1 ggcgccagtt cgtggccaca ggagtggccc gcaccgtgga cgacacccga ccggcgttcg
       61 cggtggcctc gtacggctgc caccaactcc ggtcgatcca cagccaggtc ggtacctgaa
      121 catacagacg cccgcgctcc gatgggctcg tccggatatc cggcggagcg atcctcaccg
      181 acgcgagcgc atcaagtgcc aactggtagg ggtcgacaag atcgccctcg ggcctcagga
      241 agaacccgaa gatcccaccc tggccgtcgc accaccgccc gagccagcgg ccggtggtcg
      301 agtgctgcgg ctcgcccgtg atcgggtggt acaccgtgaa ggcgaagtcg tcggcgacga
      361 ccacccgcca agggcagggt ttctcgcctg aggcgccccc gctcgatccg ccgttaccac
      421 cgccgcctcc gccattaccg ccgccgtttc ccttgcccag cattacgctg ttttcaaaag
      481 cagttccctt gtcgagcgcg tacgcaagcg cggttgaagc gacggagact acaagaagaa
      541 gtaccagaag cgctgccaac atctttttca tcgaaaaatc ccctctctca tcatcttgaa
      601 ataggttaca tgggtataat gccactttct tcgacgaggc gcattgtaag cgttctccat
      661 ttctcgctcc gtagttcccg caacaccttc ggaagctcgg agaagagtaa aaggtttttg
      721 taagacgact ctttcagtgg acatcgcccc ggtcggacag ccgactcggg gcgatgcgag
      781 ttatttcaga acgagaccgg ttccttcacg tcaaggcgtt ctttgatggc gtcttggagc
      841 aggctggaga agttgaggtt gtgctccatg ccgagcttgt tcagccatgc gggcaacgta
      901 acgctcttgg tgatgctgcg cttcgcctcc tcctcgcgga cgatgtccat gcgaacggtc
      961 acgagtacga cggcttcacc tttcttcaca ttgatttcgt cgaaagaggt tggagccggg
     1021 atatggtccc catcacgctc ggagacgtaa agaaaacctt cgagagcctc cttggcgcga
     1081 gccacggcct ccgcttcgga cttgccgcat gtgagacatc cggggaggtc gggaaactcg
     1141 accgctattc cgttcttgtc tttcgagaat attgccgggt acgtacgcat atccttgttt
     1201 cctctattgg ccatgagtct tcctcctttt cctgaggcgg gactacttca gccccgcctg
     1261 tttgaggatg cttatcgatg ttttggggtg gatgtctttt tcagggtgtg ggaccgtgat
     1321 ctttcccttt ttcgtgtcgt gctcgaagta gtgatgatcg tctcgggctc ccgtaaagta
     1381 ccaaccgtcc tcctccagca tcttgatgat ctccctgctg ctgtagctct tgatcgg
//
LOCUS       DAOTIL010000799         1436 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminobacteriaceae bacterium isolate AMR_MDS_3026
            NODE_106944_length_1436_cov_2.830558, whole genome shotgun
            sequence.
ACCESSION   DAOTIL010000799 DAOTIL010000000
VERSION     DAOTIL010000799.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868050
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminobacteriaceae bacterium (wastewater metagenome)
  ORGANISM  Aminobacteriaceae bacterium
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae.
REFERENCE   1  (bases 1 to 1436)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1436)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:19:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,224
            CDSs (total)                      :: 3,198
            Genes (coding)                    :: 3,184
            CDSs (with protein)               :: 3,184
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1436
                     /organism="Aminobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_106944_length_1436_cov_2.830558"
                     /isolate="AMR_MDS_3026"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:3034609"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..391)
                     /gene="dcm"
                     /locus_tag="P5201_13385"
     CDS             complement(<1..391)
                     /gene="dcm"
                     /locus_tag="P5201_13385"
                     /EC_number="2.1.1.37"
                     /inference="COORDINATES: protein motif:HMM:TIGR00675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003886 - DNA
                     (cytosine-5-)-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006304 - DNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA (cytosine-5-)-methyltransferase"
                     /protein_id="HRV99584.1"
                     /translation="MSDNKQLTFIDFFSGIGGFRHGLELAGLKCVGFCEKDKFAVRSY
                     RAMYDTEGEWYGEDISKLGADDVPQADIWTAGSPCQNVSIAGGRSGLHGDRSGLFFQF
                     VDLLKGKAEADKPQWIILENVKGLLSSN"
     gene            complement(438..>1436)
                     /locus_tag="P5201_13390"
     CDS             complement(438..>1436)
                     /locus_tag="P5201_13390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008712124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific DNA-methyltransferase"
                     /protein_id="HRV99585.1"
                     /translation="NIALNKITGAWNEQLLADLLVDLQNVDFNIDLTGFEAPEVEQLF
                     SKVHDKEVKEDDFDVDEALKKPTVSKSGDLWLLGKHRLICGDSTQPETFTALMDGIKA
                     NIVVTDPPYNVNVEETAGKIKNDNMADADFYKFLFAAFVNMEQNMESDASIYVFHADT
                     QGLTFRRAFADAGFYLSGCCIWKKNALVLGRSPYQWQHEPCLFGWKKGGKHVWYSDRK
                     QTTIWEYDRPKSSKEHPTMKPVALLAYPIQNSSMRGCVVLDPFLGSGSTLMACEQTGR
                     ICYGIELDEKFADVIVNRYIEAVGGSDGVFLVRDGVKTPFAQVPKPKSDEDEIPLF"
BASE COUNT          322 a          413 c          364 g          337 t
ORIGIN      
        1 cattgctcga aagaagtccc ttaacatttt caaggataat ccattggggc ttatccgctt
       61 ccgctttccc tttgaggagg tcaacgaatt gaaaaaacag tccgcttcgg tcaccgtgta
      121 atccggatcg tccccctgca atagagacat tttgacaagg acttcccgca gtccatatat
      181 ctgcttgagg aacgtcgtct gctccgagct tggaaatgtc ctctccatac cattctccct
      241 ccgtatcgta catggcgcgg taagaccgta ccgcaaattt atccttctcg cagaatccga
      301 cgcacttgag tccggcaagc tcaaggccgt gccggaagcc gccgatcccc gaaaagaaat
      361 ctataaatgt aagctgcttg ttatcagaca tagctgtccg ctccttccaa atgaaaaagg
      421 cacgggacag cccatgccta aaatagtggt atttcgtcct cgtcgctctt cggcttcgga
      481 acctgtgcga acggcgtctt aaccccatcg cgcacgagga acactccgtc cgaaccgccg
      541 accgcttcaa tgtagcggtt cacgataacg tcggcaaact tctcgtcaag ctctatgccg
      601 tagcaaatcc gcccggtctg ctcacacgcc atcagcgtag agccggaacc gagaaagggg
      661 tcgagaacga cacagcccct catggatgag ttctgtatcg gataggcgag aagcgcaacg
      721 ggcttcatgg tcggatgctc cttcgaagac ttcgggcggt cgtactccca aatggtcgtt
      781 tgcttacggt cgctgtacca gacgtgcttc ccacccttct tccaaccgaa gagacaaggc
      841 tcgtgctgcc attgatacgg cgagcggccg aggacgagcg cgttcttttt ccagatgcag
      901 cacccggaga ggtagaaccc cgcgtcggcg aaagctctgc ggaacgtcaa accctgggtg
      961 tccgcgtgga acacatagat ggaagcgtcg ctctccatgt tctgctccat attaacgaaa
     1021 gcggcgaaca ggaacttata aaagtccgca tccgccatgt tgtcgttctt gattttaccc
     1081 gccgtctctt caacattcac gttgtagggc gggtcggtca ccacaatgtt tgcctttata
     1141 ccgtccatga gagcagtaaa ggtttccggc tgcgtggagt cgccgcagat gagtcggtgt
     1201 ttaccgagca accacagatc gccggacttc gagacggtcg gtttcttcag cgcctcgtcc
     1261 acgtcgaagt cgtcttcctt gacctcttta tcgtgtactt tggaaaagag ctgctcgacc
     1321 tccggggcct caaagcctgt gaggtcgata ttgaagtcca cgttctgcag gtcaacaagc
     1381 agatcggcca ggagctgttc gttccaggcg cccgtgattt tattgagcgc gatgtt
//
LOCUS       DAOTIL010000800         1435 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminobacteriaceae bacterium isolate AMR_MDS_3026
            NODE_107032_length_1435_cov_3.690580, whole genome shotgun
            sequence.
ACCESSION   DAOTIL010000800 DAOTIL010000000
VERSION     DAOTIL010000800.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868050
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminobacteriaceae bacterium (wastewater metagenome)
  ORGANISM  Aminobacteriaceae bacterium
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae.
REFERENCE   1  (bases 1 to 1435)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1435)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:19:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,224
            CDSs (total)                      :: 3,198
            Genes (coding)                    :: 3,184
            CDSs (with protein)               :: 3,184
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1435
                     /organism="Aminobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_107032_length_1435_cov_3.690580"
                     /isolate="AMR_MDS_3026"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:3034609"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            132..1421
                     /locus_tag="P5201_13395"
     CDS             132..1421
                     /locus_tag="P5201_13395"
                     /inference="COORDINATES: protein motif:HMM:TIGR03960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03960 family B12-binding radical SAM
                     protein"
                     /protein_id="HRV99586.1"
                     /translation="MKCRNESAAPEQRAVPREEAVQPALFEYGDGMWPLLSNVKRSSR
                     YAGCEYGVIPPKDGNSIVRFCLAFPDVYEIGMSYLGFQILYFLLKNLPCGDADRVYCP
                     WTDLEEQMRARETPLTSLESGRPLREFDVVGFTLQYELSYTNILTMLDLGRIPLLSAD
                     RGENAPLVAAGGVGALSPEPLAPFIDFFCVGDGEELLPEVITLLSGAKGLSRREKLEL
                     LSGIEGVYVPSLVECDVGEGGVSFRSAAEKRVALPVRRRIVESLDDAFFPEMLLVPSG
                     GIVHDRVAVELFRGCTRGCRFCQAGMVTRPVRERSVRPLAERIVSLVEKTGWEEVGLL
                     SLASCDYSGIGELVRELSPMLAEKNVKLSLPSLRMDAFSVNLAAEMESMRRGGITFAP
                     EAGTQRLRDVINKGVSEEDFENSLRTAFTKGWDRVKL"
BASE COUNT          254 a          428 c          461 g          292 t
ORIGIN      
        1 cttgctcaga gcgtgtacgt ttcgacgctg aaaagatact gagcatcgat tccgagccgg
       61 gaacaatcgg ggcggaaccg tgaagaatcc tgctgtcggc aggtgtgaaa tcgagggagg
      121 aattgcaatc tatgaagtgc agaaacgaat ccgcggcgcc ggagcagcgt gccgtcccgc
      181 gcgaagaagc cgttcagccg gctctcttcg agtacggcga cgggatgtgg ccgctcctct
      241 ccaatgtgaa gcgctcctcc cggtacgcgg ggtgcgagta cggcgtcata cctcccaagg
      301 acgggaattc cattgtgcgt ttctgcctcg ctttccccga tgtgtacgag ataggaatga
      361 gctatctcgg attccagatc ctctacttcc tcctgaagaa tctcccgtgc ggcgacgcgg
      421 accgggtcta ctgcccgtgg accgacctcg aggagcagat gcgggcgcgg gagacgccgc
      481 tgacctccct tgaaagcggc cgtcccctcc gcgagttcga cgtggtcggt ttcaccttgc
      541 agtatgaact gagctacacc aacatcctca ccatgctcga ccttgggcgt atcccgctgc
      601 tttcggccga caggggcgag aatgcccccc tcgtcgcggc gggaggcgtc ggcgcgctct
      661 ccccggaacc gctcgctccg tttatcgact tcttctgcgt cggcgacgga gaggagctgc
      721 ttccggaggt gatcacgctt ctttccggcg ccaaggggct ctcccggaga gaaaaacttg
      781 agttgctctc cgggatagaa ggggtgtacg ttccctctct tgtcgaatgc gacgtcggcg
      841 agggaggcgt cagtttccgt tctgcggcgg agaagcgcgt cgcgctcccc gttcggcggc
      901 gcatcgtcga atcgctggac gacgcctttt tcccggagat gctcctcgtg ccgtcaggag
      961 gaattgtcca cgaccgtgtt gccgtggaac tcttccgcgg ctgtaccagg ggctgccgtt
     1021 tctgccaggc gggcatggtg acccgtcccg tcagggagcg ctccgtccgt cccctcgcgg
     1081 agcgaatcgt ttcgctggtc gagaagacgg ggtgggagga ggttggtctc ctttccctcg
     1141 catcctgcga ctactccggc atcggcgaac tcgtcaggga gctctcgccg atgctggcgg
     1201 aaaagaacgt caaactgagc ctgccgagcc tccggatgga cgcattctcg gtgaatcttg
     1261 ccgcggagat ggagtcgatg cggcgcggag gtatcacctt cgctccggaa gcaggtacgc
     1321 agcgtcttcg cgacgtcatc aacaagggag tgagcgaaga ggatttcgag aactctctcc
     1381 gcaccgcgtt cacgaaaggg tgggacaggg tgaagctcta gttcatgatg gggct
//
LOCUS       DAOTIL010000801         1435 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminobacteriaceae bacterium isolate AMR_MDS_3026
            NODE_107047_length_1435_cov_3.003623, whole genome shotgun
            sequence.
ACCESSION   DAOTIL010000801 DAOTIL010000000
VERSION     DAOTIL010000801.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868050
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminobacteriaceae bacterium (wastewater metagenome)
  ORGANISM  Aminobacteriaceae bacterium
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae.
REFERENCE   1  (bases 1 to 1435)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1435)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:19:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,224
            CDSs (total)                      :: 3,198
            Genes (coding)                    :: 3,184
            CDSs (with protein)               :: 3,184
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1435
                     /organism="Aminobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_107047_length_1435_cov_3.003623"
                     /isolate="AMR_MDS_3026"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:3034609"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..939)
                     /locus_tag="P5201_13400"
     CDS             complement(<1..939)
                     /locus_tag="P5201_13400"
                     /inference="COORDINATES: protein motif:HMM:NF015754.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LCCL domain-containing protein"
                     /protein_id="HRV99587.1"
                     /translation="MKRKVLFVFICIALSLFFVEVFGAWGVGGAFAEELTLIEQQKLA
                     RTLFNKIAAKDEQDVQGIEPLFLEVIEKAPRVRRAEEAYWMLSNMYMQAFDPPRNKDA
                     VALLEQYLTRYPRSRVLDDKFSMFTTPGLPLVKGRLLSLYEQEEMWEKAAALYDEVIP
                     EPKTAGEALLSHFIGYGRAMEQTGHKEKAIAAYTVFLERSGNTGDFLEEIVKDRLKEL
                     GVEVSSDTSVADGTVPEDPGNLESFRGRNGEVFRFRVRGSALGSLWGDGVYTDDSSLA
                     TAAVHAGVLRQEEEGVVAVRILPGREKYTGVERNGVV"
     gene            complement(1021..>1435)
                     /locus_tag="P5201_13405"
     CDS             complement(1021..>1435)
                     /locus_tag="P5201_13405"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRV99588.1"
                     /translation="QAEGFERLVKAAGGDTDKAIMMMIANKLPELVKTQVEAIKGIKI
                     DKVTVWDSAGGASDKTSTANFLSGLMKSIPPLQDMFKMAGMELPAYMKGGQKNEAPWP
                     VPYEAGPERMMADAGEPVETDPHREMPPEVETEHA"
BASE COUNT          251 a          482 c          390 g          312 t
ORIGIN      
        1 gacgacgccg ttccgctcga cacctgtgta tttttcacgc cccggaagaa tccggacggc
       61 gacgactccc tcttcttcct ggcggagcac ccccgcatgc acggcggccg tcgccagact
      121 cgaatcgtcc gtgtagaccc cgtcccccca aaggctaccc agcgccgatc cccgcacgcg
      181 gaaacggaac acttcgccgt tccgtccgcg gaaggattcc agattccccg ggtcttccgg
      241 aaccgttcca tccgcaacgg atgtgtccga ggagacttcg acccccaact ccttgaggcg
      301 atccttcacg atctcctcaa ggaagtcccc cgtatttccg ctgcgctcaa gaaataccgt
      361 atacgccgct atcgccttct ctttgtgtcc ggtctgctcc atggccctgc cgtacccgat
      421 gaaatggctc agcagcgcct cgccggctgt cttcggctcc ggaatgactt cgtcgtagag
      481 ggccgcggcc ttctcccaca tctcctcctg ctcgtacaaa gaaaggaggc gccctttcac
      541 aagcgggagt ccgggggtgg tgaacatgga gaacttgtcg tccagtacgc gggagcgggg
      601 gtagcgcgtc agatactgct ccagcagcgc gacggcgtct ttgtttctgg gcggatcgaa
      661 cgcctgcatg tacatgttgc tgagcatcca gtacgcctcc tcggcgcgcc ggacccgggg
      721 agccttctcg ataacctcca ggaagagagg ctcgatccct tgaacatcct gttcgtcctt
      781 ggcggctatc ttattgaaga gcgtcctcgc gagcttctgc tgctcgataa gcgtcagttc
      841 ctccgcgaac gcgcccccga cgccccacgc tccaaagact tccacgaaga agagagagag
      901 cgcgatacag atgaacacaa acaaaacctt tcttttcact cggaacacct ccccctttgt
      961 cagagcatgc gcacaactac aggatacaca cctgcattcc ttccgcggag cgttggcgct
     1021 ctacgcgtgc tccgtttcta cctccggagg catctccctg tgcgggtcgg tctcgacggg
     1081 ttcgcccgcg tcggccatca tgcgttccgg tccggcttcg tacggaacgg gccaaggcgc
     1141 ttcgttcttc tgtccgccct tcatgtaggc gggcagctcc attcccgcca ttttgaacat
     1201 gtcctggagc ggagggatgg atttcatcag accggagagg aagttcgcgg tggatgtctt
     1261 gtcgctggct cctccggcgc tgtcccagac ggtgaccttg tcgatcttga tccccttgat
     1321 ggcctcgacc tgggtcttca ccagttcggg aagcttgttg gcgatcatca tcatgatagc
     1381 tttgtcggtg tcgccgccgg ctgccttgac aagccgctcg aagccctccg cctgc
//
LOCUS       DAOTIL010000802         1431 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminobacteriaceae bacterium isolate AMR_MDS_3026
            NODE_107453_length_1431_cov_4.251453, whole genome shotgun
            sequence.
ACCESSION   DAOTIL010000802 DAOTIL010000000
VERSION     DAOTIL010000802.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868050
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminobacteriaceae bacterium (wastewater metagenome)
  ORGANISM  Aminobacteriaceae bacterium
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae.
REFERENCE   1  (bases 1 to 1431)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1431)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:19:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,224
            CDSs (total)                      :: 3,198
            Genes (coding)                    :: 3,184
            CDSs (with protein)               :: 3,184
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1431
                     /organism="Aminobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_107453_length_1431_cov_4.251453"
                     /isolate="AMR_MDS_3026"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:3034609"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..346
                     /locus_tag="P5201_13410"
     CDS             <1..346
                     /locus_tag="P5201_13410"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRV99589.1"
                     /translation="EKAEADFFRSKKKYVDTARALVKEFFGTLAAKDLAKSGFKPRFR
                     SGMLGRLSKQFPHDLLMLLDYCDFSLARHPGYVLDETPGNVAAPALAGILASRLVALD
                     GFLFSFRQHEDD"
     gene            414..>1431
                     /locus_tag="P5201_13415"
     CDS             414..>1431
                     /locus_tag="P5201_13415"
                     /inference="COORDINATES: protein motif:HMM:NF012234.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HRV99590.1"
                     /translation="MGESVREKKARNRSGGSTLGKKGRDLTALALSGELLPPIGRERE
                     CAEVLSTLCRMEKGNPLLLGDAGVGKTAVAEGVACLLASGNAPADLRNASVVEIKLSA
                     FIEAKRFSGGEDFVNSLAEEVLSDNRETIQTVLFVDGLHSLFLDERSWSDWFKPYLEQ
                     TKFRIIGTTTHKALGRTFERNDVFFRYFSPVFIREFDPETAVEALRGKGALLEAYHAV
                     LIPYDTAATAVELSQRYLPGRRLPEKAIDILDSACVLARNTGEVTREHVRAAVAGLAN
                     LPPEAVACSFADICKDLGERLAQRVKGQPEALKKVSRLLKRRTAGFDRRRERPLGSFL
                     FIGPT"
BASE COUNT          282 a          372 c          474 g          303 t
ORIGIN      
        1 cgaaaaggcg gaggcggact tttttaggtc gaagaagaag tacgtggaca ccgcgcgcgc
       61 tttggtgaag gagttctttg gtacgctggc ggcaaaagat cttgcaaagt cgggcttcaa
      121 accgagattc aggagcggaa tgctgggaag gctttcaaag caatttccgc atgatctcct
      181 gatgcttctc gattactgtg atttttcctt ggcgaggcat cccgggtatg tactggacga
      241 aacgccgggc aacgtcgccg ctccggcgct cgcgggcatt ctcgcgtcgc ggcttgttgc
      301 tctggacggc tttctgtttt ctttcaggca gcatgaggac gactaagcgc cgcgttccgt
      361 ttccgtgttc gccgtagata ttcaacattc cacgccggaa aggaggatgc ttcgtgggcg
      421 aaagcgtgcg ggaaaaaaaa gcgcggaatc gttcaggcgg ttccaccttg ggaaagaaag
      481 ggcgggatct gaccgcgctc gcattgtcgg gtgaactgct tcctccgata gggcgggaaa
      541 gagagtgcgc cgaggtgctt tcgacgctgt gccgcatgga gaaggggaat cctctgttgc
      601 tgggcgatgc cggcgtgggg aagacggcgg tggcggaggg ggtggcgtgt cttcttgctt
      661 ccggaaatgc gccggccgat ctgcggaacg caagcgtggt ggagatcaag ttgtcggctt
      721 ttatcgaagc caaaaggttc agcggcggag aggatttcgt caactccctc gccgaggagg
      781 tgctgtccga caacagagag acgatacaaa cggttctctt cgttgacggg cttcattcgt
      841 tgttcttgga tgaacgctcc tggagcgact ggttcaagcc gtaccttgaa caaacaaagt
      901 tccgcataat cgggacgacg acccacaaag ccctcgggcg gacgttcgag aggaacgacg
      961 tgttcttccg gtacttttcc cctgttttca tcagggagtt cgatcccgaa acagccgtgg
     1021 aggcgcttcg ggggaaagga gcccttcttg aggcgtatca cgcggtgctc atcccttacg
     1081 atacggctgc gaccgctgtt gaactgtcgc agcgctacct gcccgggcgg cggcttccgg
     1141 aaaaagccat cgacatcctt gattcggcct gcgtactggc acggaacacg ggggaagtga
     1201 cgcgggagca tgtgcgcgcg gcggtcgccg gattggcgaa cctgcctccg gaggctgtgg
     1261 cgtgttcctt cgcggatatc tgcaaggacc tcggagaacg ccttgcgcaa cgggtgaagg
     1321 gacagccaga ggccttgaag aaggtcagca ggctgttgaa acgccgtacc gccggtttcg
     1381 accggaggcg ggagcggcct ctcgggagct tccttttcat cgggcccacc g
//
LOCUS       DAOTIL010000803         1430 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminobacteriaceae bacterium isolate AMR_MDS_3026
            NODE_107587_length_1430_cov_4.152727, whole genome shotgun
            sequence.
ACCESSION   DAOTIL010000803 DAOTIL010000000
VERSION     DAOTIL010000803.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868050
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminobacteriaceae bacterium (wastewater metagenome)
  ORGANISM  Aminobacteriaceae bacterium
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae.
REFERENCE   1  (bases 1 to 1430)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1430)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:19:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,224
            CDSs (total)                      :: 3,198
            Genes (coding)                    :: 3,184
            CDSs (with protein)               :: 3,184
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1430
                     /organism="Aminobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_107587_length_1430_cov_4.152727"
                     /isolate="AMR_MDS_3026"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:3034609"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(71..1243)
                     /locus_tag="P5201_13420"
     CDS             complement(71..1243)
                     /locus_tag="P5201_13420"
                     /inference="COORDINATES: protein motif:HMM:NF024813.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH domain-containing protein"
                     /protein_id="HRV99591.1"
                     /translation="MAMIQVVRFDPELDPGRVLAWRYRNPEDPARGDELGTWAQLVVE
                     ESQEAILFRNGQALDLFGPGRHTLKTANMPLLSKAVNLPFGGESPFKAWVWFVNKVHS
                     LDVKWGTPSPVQLQDPKYGVLLPVRSYGQFGVRIEDSRKFLVKLVGARDQFDREALAH
                     HFRGLLLTRIQDLISTYLVHRGISVVEVNAWLDEISEEIHGKIAGLFGDYGIEVLNFF
                     VNSINVPEDDPAVAELKKTLSERAKMEVLGYNYQQMRSFDTLEKAASNEGGGNLMNAG
                     LGLGVGMGVGGAFGRLAGEMGSNLTTGGGEPVPSARCAKCGASAGRGAKFCSECGASL
                     PGRVCPSCGKPLPEGAKFCAECGASLVPRCPSCGKEASPGARFCAECGSAIGRKEE"
BASE COUNT          302 a          485 c          357 g          286 t
ORIGIN      
        1 tactgaacat cctctctggt gtaatgaaca tagtagctaa ctactccctt cttgaaatgc
       61 cgaatacctg ttactcttct ttccggccga tggcggaacc gcactcggcg cagaatctgg
      121 cgccgggaga cgcctccttc ccgcaggaag ggcatctcgg aaccagcgaa gcgccgcact
      181 ccgcacaaaa cttcgccccc tccggcagcg gtttgccgca tgacggacag actcttccgg
      241 gaagagaagc gccgcactcc gagcagaact tcgccccccg gcctgcagaa gcgccgcact
      301 tcgcgcatcg ggccgacgga accggttctc cccctccggt ggtcagattg ctccccattt
      361 cgccggccag tctgccgaac gctccgccga ctcccatccc tactccaagt cccagaccgg
      421 cgttcatcag attgccgccc ccctcgttcg acgcagcttt ttcaagggta tcgaacgagc
      481 gcatctgctg gtaattgtac cccagaacct ccatttttgc ccgttcggag agcgtctttt
      541 tcagctccgc caccgcagga tcgtcctccg ggacgttgat cgagttcacg aaaaagttga
      601 gcacttcgat tccgtaatcc ccgaacagcc ccgcgatctt cccatgaatc tcctcggaga
      661 tttcgtcaag ccaggcgttc acctcaacaa cggagatacc gcgatgcaca agatacgtcg
      721 agataagatc ctgtatccgc gtgagcagca acccccggaa gtgatgagcc agcgcctctc
      781 ggtcgaactg atcacgcgcg cccaccagct ttacgaggaa cttgcgcgaa tcttcgatgc
      841 ggacgccgaa ctgtccgtac gacctgaccg ggagcaggac gccgtatttg ggatcctgaa
      901 gctgcacagg agagggcgtg ccccacttca cgtcaagtga atgcaccttg ttcacgaacc
      961 agacccacgc cttgaatggc gactctccgc cgaagggcag attgaccgcc ttcgaaagca
     1021 acggcatgtt cgccgtcttc aacgtatgcc ttccgggacc gaaaaggtcg agcgcctgcc
     1081 cgttccggaa gaggatcgcc tcctgcgact cctccactac aagctgcgcc cacgtcccca
     1141 gttcatcgcc cctcgccgga tcctccggat tccggtagcg ccacgccaaa accctccccg
     1201 ggtcgagttc cgggtcgaac cggaccactt gaatcatcgc catcgcaccc accgccttcg
     1261 ttcggagtta tccctcggga gcatatcgct gtatctcttc gaatgatatt atgctaccat
     1321 accgccagtc ccgtaaagga gagccggacg aaaatccagc agagcgggac gttttcttga
     1381 agggaatgat tataagctat ggatgaacag cagctgctct ctctccttcg
//
LOCUS       DAOTIL010000804         1430 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminobacteriaceae bacterium isolate AMR_MDS_3026
            NODE_107626_length_1430_cov_2.767273, whole genome shotgun
            sequence.
ACCESSION   DAOTIL010000804 DAOTIL010000000
VERSION     DAOTIL010000804.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868050
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminobacteriaceae bacterium (wastewater metagenome)
  ORGANISM  Aminobacteriaceae bacterium
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae.
REFERENCE   1  (bases 1 to 1430)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1430)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:19:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,224
            CDSs (total)                      :: 3,198
            Genes (coding)                    :: 3,184
            CDSs (with protein)               :: 3,184
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1430
                     /organism="Aminobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_107626_length_1430_cov_2.767273"
                     /isolate="AMR_MDS_3026"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:3034609"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1077)
                     /locus_tag="P5201_13425"
     CDS             complement(<1..1077)
                     /locus_tag="P5201_13425"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012792.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HRV99592.1"
                     /translation="MFRIRRVFDALSPANVRAIARVQEILREQFPEARPGEADAIPEK
                     LSNHLKYGFQTILFVAEKGQNKVHGFALILYDPLLKFCYLDYIAVAGREAGSGLGSIL
                     YERVRKEALDLGCVGVFFECLPDDPALSRNDTVRKQNVARLRFYERYGATPLVGTKYE
                     TPLEPEGDNPPYLLFDGLGKKEPLRREKARLVVAAILGKKYGALCSPEYIRMVMDSFR
                     DDPVKLRPLRYVGAPTRAAVASELPESERIALFVNRDHAIHHVRERGYVESPVRVEAI
                     LKEIVPSGLFSEKKPRHFGEKHILKVHGAVFFHYLRKVCLSMPEGESVYPYVFPVRNA
                     ARPPQDLSVRAGYFCMDTFTPLNKN"
     gene            complement(1095..>1430)
                     /locus_tag="P5201_13430"
     CDS             complement(1095..>1430)
                     /locus_tag="P5201_13430"
                     /inference="COORDINATES: protein motif:HMM:NF024304.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase family
                     protein"
                     /protein_id="HRV99593.1"
                     /translation="EGAIYFHCAPEGRKLDVLRRNPKACFQVETDTELVTSTRACGWG
                     MLYRSVVVSGVVSVVESLEEKARGLTALMKKCAGDAFSHEFTEQELGAVVVLRLEPVS
                     RTGKARRPA"
BASE COUNT          266 a          452 c          452 g          260 t
ORIGIN      
        1 gtttttgttc agcggggtga aggtgtccat gcagaagtat ccggcccgca ccgagagatc
       61 ctgcggcggc cgggcggcgt tccgcaccgg gaagacgtac gggtagacgg actccccctc
      121 cggcatggag aggcagacct ttcgcagata gtggaaaaaa accgcgccgt ggaccttcaa
      181 gatgtgcttt tcgccgaagt gacggggctt cttctcggag aagagcccgg acgggacgat
      241 ctccttgagg atggcctcca cgcggaccgg cgattcgacg tatccacgct cgcgcacgtg
      301 gtgaatcgca tggtcgcggt tcacgaagag ggcgatgcgc tcgctttccg ggagttccga
      361 ggcgaccgcg gcgcgcgtcg gcgcaccgac gtagcgcagc ggacgcagct tcacggggtc
      421 gtccctgaag gaatccatca ccatgcggat atactcgggc gaacagagag cgccgtactt
      481 cttgccaagg atcgccgcga cgacaagccg cgctttctcc ctgcggagcg gctctttttt
      541 cccgaggccg tcgaagagga ggtacggcgg attgtcgccc tcgggctcca gcggggtctc
      601 gtacttcgtc ccgacgagcg gcgtcgcccc gtaccgctcg tagaagcgca ggcgggcgac
      661 gttctgcttt cgcacggtat cgttccggct cagcgccggg tcgtcgggga ggcattcgaa
      721 gaagacgccg acgcatccaa ggtcgagcgc ctccttccgg accctctcgt agaggatgct
      781 cccgaggccg ctccccgctt cgcgcccggc gacggcgata tagtccagat agcagaactt
      841 cagtagcgga tcatagagga tgagggcaaa gccgtgcact ttgttctgcc ccttctccgc
      901 aacgaagaga atagtctgaa aaccgtactt caggtggttc gagagcttct ctgggatcgc
      961 gtcggcctcg ccgggacggg cttcgggaaa ctgctcccgc aggatctcct gaacacgcgc
     1021 aatcgccctt acgttcgcag gggagagcgc gtcgaaaacc ctccggatgc ggaacatagc
     1081 ccctcgctcc cgaatcaggc cggacggcgg gctttccccg tcctggagac cggttcgagc
     1141 cggaggacaa cgacagcacc cagttcctgc tccgtaaact catgggagaa ggcgtctccc
     1201 gcgcatttct tcatcaatgc agtcaatccg cgcgccttct cttcgagaga ctcgacgacg
     1261 gagacgacgc cggaaaccac gacgcttcgg tagagcattc cccagccgca tgccctggtg
     1321 gaggtgacga gttcggtatc ggtttccacc tgaaaacaag ccttcgggtt gcgccgcagc
     1381 acgtcgagct tccgcccttc cggggcgcag tggaagtaga tcgccccttc
//
LOCUS       DAOTIL010000805         1430 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminobacteriaceae bacterium isolate AMR_MDS_3026
            NODE_107662_length_1430_cov_2.133091, whole genome shotgun
            sequence.
ACCESSION   DAOTIL010000805 DAOTIL010000000
VERSION     DAOTIL010000805.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868050
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminobacteriaceae bacterium (wastewater metagenome)
  ORGANISM  Aminobacteriaceae bacterium
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae.
REFERENCE   1  (bases 1 to 1430)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1430)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:19:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,224
            CDSs (total)                      :: 3,198
            Genes (coding)                    :: 3,184
            CDSs (with protein)               :: 3,184
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1430
                     /organism="Aminobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_107662_length_1430_cov_2.133091"
                     /isolate="AMR_MDS_3026"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:3034609"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..850
                     /locus_tag="P5201_13435"
     CDS             <1..850
                     /locus_tag="P5201_13435"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRV99594.1"
                     /translation="LRMTGLSPEKTDSSGIFRREKRALYLENREREARKEIGKLILEE
                     ALRTGVLCSKTSFVAVHEKAGSVVSRSLVVANALPRGWDAGFAASARAMSMQGASLTG
                     GFFAPSNAPRMSMRIASMPGSSAPEDMDCFSVCGNSAPPAEEMPISMSMPRLRRAARS
                     GKSASPEGTGPGIEVFNGPLPREEGLLFEGSAGAGVLQGVSLLTRLVLKTGGKDAKKR
                     LEGSLLRLLINGAPVASVALADMIAFGGERPLNVRLREGDRLEVRISGYQADEDVRIS
                     LFLRTT"
     gene            complement(1258..>1430)
                     /locus_tag="P5201_13440"
     CDS             complement(1258..>1430)
                     /locus_tag="P5201_13440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000147566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HRV99595.1"
                     /translation="KQKLPADKRKELRTRASNGEGKAALAREFGISRETLYQYLRENQ
                     GVGEEYQKTEFH"
BASE COUNT          272 a          429 c          460 g          269 t
ORIGIN      
        1 cctccgcatg accgggcttt cccccgagaa gacggattcc tccggtatct tccgtcggga
       61 aaagagggcg ctctaccttg agaacaggga gagggaggcg cgaaaagaga tcgggaaact
      121 gatcctcgag gaagcgctcc gaacgggggt gctctgttcg aagacctcct tcgtcgccgt
      181 gcacgaaaag gctgggagtg tcgtatcgcg ctcgctggtc gtcgccaacg cgctccccag
      241 agggtgggac gcgggcttcg ccgcaagcgc aagggcgatg agcatgcagg gagcatcgct
      301 gaccggagga ttcttcgcgc cttccaacgc tccgcggatg tccatgcgca tcgcctccat
      361 gccaggaagc tccgctccgg aggatatgga ctgcttctcg gtgtgcggga attcggctcc
      421 tcccgccgag gaaatgccga tctccatgag catgccgcgt ttaagaagag cggcgcgctc
      481 cgggaagagc gcatccccgg agggtacggg cccgggaatc gaggtcttca acggaccgct
      541 gcctcgcgaa gaggggcttc tcttcgaggg aagcgccggg gccggcgttc tgcaaggggt
      601 ctccctgctg acgcgcctcg tactgaagac cggcgggaag gacgcgaaga agcgcctcga
      661 agggagtctg ctgcgtctgc tgatcaacgg cgcccccgtc gcttcggtcg ccctcgccga
      721 catgatcgcc ttcggaggcg aacgccccct gaacgtacga ctgagggaag gggacaggct
      781 ggaggtgcgc atctcggggt atcaggcgga cgaggatgtg cgaatctcgc tctttctcag
      841 gacgacgtaa ggcggcgacg aaaggaggcg ctgctccgcc gtcgtttccg atagtgtttg
      901 tcgaagttgc cccccgccaa aggcggaggg ggaaccgata cctttttatt cactgatgag
      961 tgccaagaca tcgtggactg ctattccctt acgctcggac acgccctggt accgaaggag
     1021 gacgaaaaca ccttctaccg cttcggatgg tcgggatcat gcggcgacga tcatcgcaca
     1081 ctcggtcgac ggatacggag acccggtcga tcgggtgtgc ggcctcgaac agagggcggt
     1141 gaaccgccat gccgaatcgg tacgccagga cggaaagatc gagtagacgg agggggcgtc
     1201 gccctcgaca ggaaacgaga ggcttccagc ccgcggacct tgttccaccc tctttggtca
     1261 atgaaactca gttttttgat actcttctcc gacgccctgg ttctccctca gatattgata
     1321 gagcgtctcc ctcgaaatcc cgaactccct cgccagagcg gccttccctt caccattgga
     1381 ggcccttgtc cgaagttctt tgcgcttgtc ggccgggagc ttctgtttgc
//
LOCUS       DAOTIL010000806         1429 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminobacteriaceae bacterium isolate AMR_MDS_3026
            NODE_107713_length_1429_cov_3.828239, whole genome shotgun
            sequence.
ACCESSION   DAOTIL010000806 DAOTIL010000000
VERSION     DAOTIL010000806.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868050
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminobacteriaceae bacterium (wastewater metagenome)
  ORGANISM  Aminobacteriaceae bacterium
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae.
REFERENCE   1  (bases 1 to 1429)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1429)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:19:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,224
            CDSs (total)                      :: 3,198
            Genes (coding)                    :: 3,184
            CDSs (with protein)               :: 3,184
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1429
                     /organism="Aminobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_107713_length_1429_cov_3.828239"
                     /isolate="AMR_MDS_3026"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:3034609"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..412)
                     /locus_tag="P5201_13445"
     CDS             complement(<1..412)
                     /locus_tag="P5201_13445"
                     /inference="COORDINATES: protein motif:HMM:NF013832.2"
                     /note="YrbG; inner membrane protein involved in cell
                     envelope integrity; putative sodium ion/calcium ion
                     exchanger; in E. coli it is non essential for cell
                     viability; member of the YRBG family of cation/Ca2+
                     exchangers; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="calcium/sodium antiporter"
                     /protein_id="HRV99596.1"
                     /translation="MINLLATLIGFIPLIYGADILVDGASSLAKRYNIPNIVIGLTIV
                     AFGTSAPEFIVNFFASLSGNTGIVLGNVLGSNIVNIMGILGVSAVIYPLAVKSNTTWI
                     EIPLSLLAAVAILVMGNDIFIDNAAWSGLSRIDGI"
     gene            complement(478..1185)
                     /locus_tag="P5201_13450"
     CDS             complement(478..1185)
                     /locus_tag="P5201_13450"
                     /inference="COORDINATES: protein motif:HMM:NF024811.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family phosphatase"
                     /protein_id="HRV99597.1"
                     /translation="MKNAESRSIGVVFDFNGTLFWDTEYQERSWDTYLAKYGVELTKE
                     EKLTRIHGRNGKDSFEYIFKRELGREEVARLIEEKEEYYREECEQQEVALAPGAVPLI
                     EDLLERGVSIAIATAAGKTNVEYFIRKFDLLAHFARERIVYNDGTIAGKPAPDLFLEA
                     MRRLGTEDRNTTVFEDSYAGIQAAENAHAGNIIIVNSNNESYPFAYKTIRHFDEVDRE
                     TDLTPPFRRAASGEPRG"
     gene            complement(1230..>1429)
                     /locus_tag="P5201_13455"
     CDS             complement(1230..>1429)
                     /locus_tag="P5201_13455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013049219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="class IV aminotransferase"
                     /protein_id="HRV99598.1"
                     /translation="GMKIEERCPLLSELAAAEEAFITGSVKEVVPVVKIGDQVIGSGE
                     PGPVTKMLHYTMLEEIVRWLE"
BASE COUNT          258 a          419 c          420 g          332 t
ORIGIN      
        1 cgatgccgtc tatcctcgag aggccggacc aagccgcgtt gtcgatgaag atgtcgttgc
       61 ccatgacgag gatggcgacg gcggcgagaa gcgagagggg gatttctatc cacgtggtgt
      121 tcgatttgac cgccaggggg tagatgacgg cggagactcc gaggattccc atgatgttca
      181 cgatattgct gccgagcacg ttcccgagca cgatccctgt gttccccgac agcgaagcga
      241 agaagttgac gatgaactcg ggcgcggacg tgccgaacgc gacgatcgtg aggccgatga
      301 cgatgttcgg tatgttgtat cgcttcgcca gcgacgacgc gccgtcgacg agtatgtccg
      361 ccccgtagat cagggggatg aaaccgatca gggttgcaag cagattgatc atagtacaga
      421 acacctcttt ttacaggtat gattttttaa tgaagcgctc tcccgcctct tttgttttca
      481 cccgcgcggt tcaccggagg cggcgcgccg aaagggcggc gtcaggtcgg tttcccggtc
      541 gacctcgtcg aaatgccgga ttgtcttata ggcaaagggg taggactcgt tgttggagtt
      601 cacgatgatg atgttcccgg catgcgcgtt ttccgccgcc tgaattcccg cgtaggaatc
      661 ctcgaagacc gtcgtgttcc tgtcctctgt tcccaggcgc cgcatcgcct ccagaaaaag
      721 gtcgggagcg ggtttcccgg ctattgttcc gtcgttgtag acgatgcgct cgcgggcgaa
      781 gtgggcgagc aggtcgaact tgcggatgaa gtactccacg ttggtcttcc ccgccgcggt
      841 ggcgatcgcg atggagacgc cgcgttccag cagatcctcg atgagcggca cggctccggg
      901 cgccaacgcg acctcctgct gttcacactc ctcgcggtag tactcttcct tctcttctat
      961 gaggcgggcg acttcctccc tgccgagttc tcttttgaag atatactcga aggaatcctt
     1021 cccgtttctg ccgtgaatac gggtcagctt ctcctctttg gtgagttcga cgccgtactt
     1081 ggcaaggtac gtgtcccatg agcgctcctg gtactccgta tcccagaaga gcgtgccgtt
     1141 gaagtcgaag acgacgccga tgctgcggct ctccgcgttt ttcatcgctc ttgttcagcc
     1201 tcctcgttgt tcctgctgcg ccggaagtgt tactccagcc agcggacgat ctcctcaagc
     1261 atcgtgtagt gaagcatctt tgtcacaggg cctggctctc ctgagccgat aacctgatcg
     1321 cctatcttca ccacgggaac gacctccttc accgagccgg tgatgaacgc ctcttccgcg
     1381 gcggcaagct ccgaaaggag agggcaccgc tcctcgatct tcatcccct
//
LOCUS       DAOTIL010000807         1426 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminobacteriaceae bacterium isolate AMR_MDS_3026
            NODE_108062_length_1426_cov_3.390226, whole genome shotgun
            sequence.
ACCESSION   DAOTIL010000807 DAOTIL010000000
VERSION     DAOTIL010000807.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868050
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminobacteriaceae bacterium (wastewater metagenome)
  ORGANISM  Aminobacteriaceae bacterium
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae.
REFERENCE   1  (bases 1 to 1426)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1426)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:19:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,224
            CDSs (total)                      :: 3,198
            Genes (coding)                    :: 3,184
            CDSs (with protein)               :: 3,184
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1426
                     /organism="Aminobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_108062_length_1426_cov_3.390226"
                     /isolate="AMR_MDS_3026"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:3034609"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            482..1228
                     /locus_tag="P5201_13460"
     CDS             482..1228
                     /locus_tag="P5201_13460"
                     /inference="COORDINATES: protein motif:HMM:NF014826.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PHP domain-containing protein"
                     /protein_id="HRV99599.1"
                     /translation="MRLLSKTPLFDLHNHTLASGHAYGTIRESAEAAADRGLAVLGFS
                     EHAWTVTGAPSRSYFLNFKEIPSRICGVRIVNGIEANILNESGEIDVDEQLGSRLDFV
                     IASLHPSPYYSGPIAKKEIIRAYIETMRRNPFVRIIGHPEDGRFPVDYRSLAEAASDR
                     GVALELNNSGLREKHGRQNGAANAEELLEECACCGAFVVMGSDSHWGGDAGALANCET
                     LIEQTGFPKELVLNYRDDGLAFILRNPLEE"
     gene            1234..>1426
                     /locus_tag="P5201_13465"
     CDS             1234..>1426
                     /locus_tag="P5201_13465"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRV99600.1"
                     /translation="MEKTHGLFPVLDIDDMTWFSADHHFGHRSVLKFDRRPYADLDEM
                     ERDLVMKHNATVPPNGATVI"
BASE COUNT          333 a          390 c          385 g          318 t
ORIGIN      
        1 cctggtgctc ttccactatt tcgcgcagtg tttcagaaaa gttcttctcc atcccgttcc
       61 ctcaccccca aatatgtacc ttccaaggtt atagcacgcc tgctgcccac tgtccacatg
      121 ttttcattac aaaatggaac aaatgtatca aagatagagt gagctgcatt tacaacctcc
      181 gattccaaga ggttttgact gtgacctgaa ttttcatctt gctccgtatc aggcggaatg
      241 gagagactgc cggaggaata cgcagagtgt tgttttttaa ttttggtgaa aagtggacac
      301 cggtcacact cggaaggccg caaacaagga gaatagagaa tgccattttt cggcagctac
      361 gagagtatgg ttgtatcgga acgactcggt tgttagaatg agagtcaaat caagaaaacg
      421 ggtaacggcg ctcaactcaa cggggcggcc ttcaatcgcc ggaaaggttg gtttgaaacc
      481 gatgcgatta ctctcgaaaa cgcctctctt cgacctgcac aaccatactc tggccagcgg
      541 ccacgcgtac ggaacaatcc gcgagtccgc cgaagcagcc gctgaccggg gactcgccgt
      601 gctcggtttc tccgaacatg cgtggactgt cactggcgct ccttcccgct cgtacttcct
      661 caacttcaag gagattcctt cccggatctg cggtgtccgc atcgtgaacg gaatcgaagc
      721 caacattttg aacgaaagcg gagagatcga cgtcgatgag cagcttggat cgcgccttga
      781 tttcgttatc gcgtcgctcc acccgtctcc ttactattct gggccgattg cgaaaaagga
      841 gataatccgg gcgtacatcg aaacgatgcg ccgcaacccc ttcgtcagga ttatcggaca
      901 cccggaggac ggacgtttcc cggtcgatta ccggtcgctc gccgaagcag cttcggaccg
      961 aggcgtcgct cttgaactga acaactccgg tcttcgcgag aaacacggac ggcaaaacgg
     1021 cgccgccaat gctgaggaac ttcttgaaga gtgtgcgtgc tgcggcgcgt tcgtagtcat
     1081 ggggagcgat tcgcactggg gaggcgacgc gggagcgctc gcaaactgcg agacgcttat
     1141 cgagcagacc ggcttcccga aagagcttgt tctcaactac cgcgacgacg gacttgcttt
     1201 catcttgagg aaccctttgg aggagtgacg gatatggaga aaacccacgg attatttccc
     1261 gttctcgata tcgacgacat gacatggttc agcgcggacc atcacttcgg tcaccggagc
     1321 gtgctgaagt tcgaccggcg tccgtatgcg gatctcgacg agatggagcg cgaccttgtg
     1381 atgaagcaca acgcgactgt acctccgaac ggcgcgacgg ttatct
//
LOCUS       DAOTIL010000808         1426 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminobacteriaceae bacterium isolate AMR_MDS_3026
            NODE_108064_length_1426_cov_3.185996, whole genome shotgun
            sequence.
ACCESSION   DAOTIL010000808 DAOTIL010000000
VERSION     DAOTIL010000808.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868050
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminobacteriaceae bacterium (wastewater metagenome)
  ORGANISM  Aminobacteriaceae bacterium
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae.
REFERENCE   1  (bases 1 to 1426)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1426)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:19:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,224
            CDSs (total)                      :: 3,198
            Genes (coding)                    :: 3,184
            CDSs (with protein)               :: 3,184
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1426
                     /organism="Aminobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_108064_length_1426_cov_3.185996"
                     /isolate="AMR_MDS_3026"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:3034609"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(169..1350)
                     /locus_tag="P5201_13470"
     CDS             complement(169..1350)
                     /locus_tag="P5201_13470"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HRV99601.1"
                     /translation="MKRLKLERVSHWFGRTRALDDIRLEIGASEFVAVLGPSGCGKTT
                     LLRILGGFLRPTEGTVTFGDDVYSSPLVHVPVERRDLGMVFQSFALWPHMTVRRHVEF
                     PLKSPRHKGTSEREKHEAVERTLEVTGLTSLQHRLPGELSGGQRQRVSLARAIVSKPS
                     ILLMDEPLSALDQELRIEMRREIQDIHRLTGATIVYVTHDQGEALAMADRLVIMKDGR
                     IEQAGTPREVYLHPRTPFAAGFVSKCNFVKGTWTGDRFAVQEDPSLLFDGAGVAEVFR
                     GMNVYPVRPEQFRIAARGRGLEGKIANKQYNGREIHYAVRCQGTIFTVYADAFEDYRP
                     GDSVRLSFEGTVRDDREDGLDREAFMVYNRPAHTDSTAGERAPVPHSGEAAETPRLGE
                     Q"
BASE COUNT          202 a          467 c          476 g          281 t
ORIGIN      
        1 tggggtcggg cgcgcccgac atcgtggggg cgtgctcgga aaagcccatc gcggcgagtc
       61 cccgttcgct cgcggcttct atgttttcct tcagcgagct gtaggcgtgc ccgctggcca
      121 gtgtgtgcgt gtgcaggtca aaaagcggtt tcatgtgatc tccctttttc attgttctcc
      181 aagccggggc gtttcggcgg cctctcccga atgcgggacg ggcgctcgtt ccccggcggt
      241 gctgtccgta tgcgccggtc tgttgtacac catgaaggcc tccctgtcca gtccgtcctc
      301 ccggtcgtcc cggacggttc cctcgaagga gaggcggacg gagtctccgg ggcggtagtc
      361 ctcgaaggcg tcggcgtaga cggtgaagat ggtcccctgg cagcgtaccg cgtagtgaat
      421 ctcccggccg ttgtactgct tgttggcgat cttcccctcc aatccccgtc cccgggcggc
      481 gatgcggaac tgctcggggc ggacggggta gacgttcatt ccgcggaaga cctccgcgac
      541 ccccgcgccg tcgaagagca gcgacgggtc ctcctgcacg gcgaaccggt ccccggtcca
      601 tgttcccttg acgaagttgc acttgctgac gaatccggcg gcgaagggcg tcctcggatg
      661 gaggtagacc tccctcggcg ttcccgcctg ttcgatgcgc ccgtccttca tgatgacgag
      721 ccggtcggcc atggcgagcg cctccccctg gtcgtgggtg acgtagacga tggtcgcgcc
      781 ggtgagcctg tggatgtcct gaatttcgcg gcgcatctcg atccgcagct cctggtcgag
      841 ggcgctcagc ggctcgtcca tcagcaggat ggagggcttg gagacgatgg cccgggccag
      901 ggagacgcgc tgccgctgcc ctccggagag ctcccccggc aggcggtgct gcagcgacgt
      961 caggcctgta acctcaaggg tccgctccac ggcctcgtgc ttctcgcgtt cgctcgtccc
     1021 cttgtggcgg gggctcttca gcggaaactc gacgtggcgg cgcaccgtca tgtgcggcca
     1081 gagcgcgaac gactgaaaga ccatgccgag gtcgcgccgc tccacgggta cgtggacgag
     1141 gggcgaggag tagacgtcgt cgccgaaggt caccgtgccc tccgtcggcc ggaggaagcc
     1201 gccgagtatg cggagcagcg tcgtcttgcc gcagccgctc ggtccgagga ccgcgacgaa
     1261 ctcggacgct cctatttcga gccgtatgtc atcgagcgcc cgcgtccggc cgaaccagtg
     1321 ggatacgcgc tccagcttca gtcgtttcat tctttttctc cttttgccgc tcgaaaagtt
     1381 ttttgtatgc cagcgcggcg ccgtagcccc caagcacgat gagcac
//
LOCUS       DAOTIL010000809         1425 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminobacteriaceae bacterium isolate AMR_MDS_3026
            NODE_108184_length_1425_cov_4.116058, whole genome shotgun
            sequence.
ACCESSION   DAOTIL010000809 DAOTIL010000000
VERSION     DAOTIL010000809.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868050
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminobacteriaceae bacterium (wastewater metagenome)
  ORGANISM  Aminobacteriaceae bacterium
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae.
REFERENCE   1  (bases 1 to 1425)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1425)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:19:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,224
            CDSs (total)                      :: 3,198
            Genes (coding)                    :: 3,184
            CDSs (with protein)               :: 3,184
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1425
                     /organism="Aminobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_108184_length_1425_cov_4.116058"
                     /isolate="AMR_MDS_3026"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:3034609"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1425)
                     /gene="ppk1"
                     /locus_tag="P5201_13475"
     CDS             complement(<1..>1425)
                     /gene="ppk1"
                     /locus_tag="P5201_13475"
                     /EC_number="2.7.4.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR03705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009358 - polyphosphate kinase complex
                     [Evidence IEA];
                     GO_function: GO:0008976 - polyphosphate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006799 - polyphosphate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyphosphate kinase 1"
                     /protein_id="HRV99602.1"
                     /translation="DGMSPSKQLALIRKRVLPLIAGAQEYWSKSLRPCLRDAGLRFVE
                     YEDLGDKHKKFLEGYFKNEIYPILTPQAIDPGRPFPVISNLSLNFLVQLADPQKGTRF
                     ARIKVPKNLSRFIFLPRNKEAKSYESLGFSPNARQCDVLLVENLVAQHLGLLFPGLQV
                     TASTTFRITRNTDVEIEEDESSDLLEAVKDLVDRRNFGEVVRLEVSSGVPKDIFLFLV
                     RNFRLAPFQIYKRKGPLDFAGMMDLCSVDRPDLKDEPLLPRTPPPFSERAPLYAVLRK
                     RDAVLYHPYDSFAPVLDFVRRAAVDPKVVAIKQTLYRVGNESPVVDALMTARKNGKQV
                     TAVVELKARFDEERNINWAEALEDVGVHVVYGLVGYKIHAKMCLVVRRETEGIKRYAH
                     IGTGNYNPSTAKVYSDLGLFTADKAICADVTDLFNAITGYSRKEDYRQLLVSPHTTRS
                     GIVGRVDREIARHKESGDGYIAFKM"
BASE COUNT          242 a          415 c          495 g          273 t
ORIGIN      
        1 catcttgaac gcaatatacc cgtctcccga ttctttatgc cgcgcgatct cgcggtcgac
       61 ccgcccgacg atgccgctcc gggttgtgtg cggggagacg agaagctggc ggtagtcctc
      121 cttgcgcgag tagccggtga tggcgttgaa aaggtcggtg acgtccgcgc agatggcctt
      181 gtccgcggtg aagagcccca ggtcggagta gaccttcgcc gtggaggggt tgtagtttcc
      241 ggtgccgatg tgcgcgtagc gcttgatgcc ctcggtttcg cgccggacca cgaggcacat
      301 cttcgcgtgg atcttgtagc cgacgaggcc gtagaccacg tggaccccca cgtcttccag
      361 cgcctcggcc cagttgatgt tccgctcctc gtcgaagcgg gccttcagct cgacgaccgc
      421 ggtgacctgc ttgccgttct tgcgggccgt catgagggcg tcgaccacgg gggattcgtt
      481 cccgacgcgg tagagcgtct gcttgatcgc caccaccttc gggtcgacgg ccgcccggcg
      541 gacgaagtcc agaacgggcg cgaagctatc gtacgggtgg tagagcacgg cgtcgcgctt
      601 gcggaggacg gcgtagagcg gcgctctctc cgaaaacggc ggaggcgtgc gcgggagcag
      661 cggctcgtcc ttcaggtcgg gccggtccac ggagcagaga tccatcatgc cggcgaagtc
      721 gagcggccct tttcgcttgt agatctggaa gggcgcgagg cggaagttcc gtacgaggaa
      781 gagaaagatg tccttcggca cgccggacga gacttcgagg cgcaccactt cgccgaagtt
      841 ccgccggtcg acgagatcct tcaccgcttc gagaaggtcg gaggattcgt cctcctcgat
      901 ctcgacgtcg gtgttccgcg tgatccggaa cgtggtggag gctgtaacct gaaggccggg
      961 gaaaagaagg ccgaggtgct gcgccaccag gttctccaca agcaggacgt cgcactggcg
     1021 ggcgttcggc gaaaagccga ggctctcgta cgacttcgcc tccttgttgc gcgggaggaa
     1081 gatgaagcgc gagaggttct tcggtacctt gatgcgcgcg aagcgcgttc ccttctgcgg
     1141 gtcggcgagc tggacgagaa agttcaggct gaggttggag atcaccggga aggggcgccc
     1201 cggatcgatg gcctgcggcg taaggatggg gtagatctcg tttttgaagt acccttcgag
     1261 gaatttttta tgcttgtcgc cgaggtcttc gtactcgacg aaacgcagtc ccgcgtcccg
     1321 caggcagggg cggagcgact tcgaccagta ctcctgcgcg cctgcgatca gcggaagcac
     1381 gcgtttccgg ataagggcga gctgcttcga gggcgacatg ccgtc
//
LOCUS       DAOTIL010000810         1423 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminobacteriaceae bacterium isolate AMR_MDS_3026
            NODE_108443_length_1423_cov_4.105994, whole genome shotgun
            sequence.
ACCESSION   DAOTIL010000810 DAOTIL010000000
VERSION     DAOTIL010000810.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868050
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminobacteriaceae bacterium (wastewater metagenome)
  ORGANISM  Aminobacteriaceae bacterium
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae.
REFERENCE   1  (bases 1 to 1423)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1423)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:19:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,224
            CDSs (total)                      :: 3,198
            Genes (coding)                    :: 3,184
            CDSs (with protein)               :: 3,184
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1423
                     /organism="Aminobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_108443_length_1423_cov_4.105994"
                     /isolate="AMR_MDS_3026"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:3034609"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..276)
                     /locus_tag="P5201_13480"
     CDS             complement(<1..276)
                     /locus_tag="P5201_13480"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein"
                     /protein_id="HRV99603.1"
                     /translation="MVSRMTNVSVRVRRKAFTLIEVFVVVALFGIIAAVALAPAVAVV
                     RRFEDVRAEEAREQRMTYFLRRIPDELRASPKEFVGEKEPAVVLVRKD"
     gene            complement(261..1136)
                     /locus_tag="P5201_13485"
     CDS             complement(261..1136)
                     /locus_tag="P5201_13485"
                     /inference="COORDINATES: protein motif:HMM:NF021445.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylglycerol lysyltransferase
                     domain-containing protein"
                     /protein_id="HRV99604.1"
                     /translation="MPFSFSSISLERADEYRQLYSQSPRKSAWYSFGSLWAWRNIYGF
                     EWAFEEGVCWIRTRNDALWAPVGPWGAIEWKKVLPRLFPEGRVFFYVPDGLGRLWREL
                     FRGGVTVGEDRSQWEYLHSVQELVELKGNRFSRKRSHFVQFVRRYPFRYRSLEGDALV
                     SVLEGQELWMKEQSVSPDLEREDAAIRELVAQRGHIPGLLAGILEVNGSPVAYTVGEA
                     LDDDTIVIHFEKALRRYSGAYQAINRMFLENVADSFTMVNREEDMGDEGMRVAKMSYH
                     PVDFLRKCTLSWFPE"
BASE COUNT          351 a          441 c          341 g          290 t
ORIGIN      
        1 gtctttacgg acaagaacca ccgcaggctc tttttctccg acgaactcct tcggagaagc
       61 gcgaagctcg tccgggatcc tccgcagaaa gtaggtcatc cgctgctccc tcgcctcctc
      121 ggcgcgaaca tcttcgaaac ggcggacaac tgcgactgcc ggagcgagcg cgaccgccgc
      181 gatgatgccg aaaagcgcga cgacgacgaa tacctcaatc agggtaaaag ctttccggcg
      241 cacccgaaca gagacgttcg tcattcggga aaccatgaga gcgtacactt cctcaaaaaa
      301 tctacagggt gataggacat cttcgctacc ctcatccctt cgtcgcccat atcctcttcg
      361 cggttcacca tcgtgaacga gtctgcgacg ttctccagaa acattctatt aatcgcttgg
      421 tatgcgccgc tatagcgcct caacgccttc tcgaaatgaa tgactatcgt atcgtcgtcg
      481 agcgcttctc caacggtata agcaaccgga gagccgttca cctcgagtat tccggcaaga
      541 agccccggga tatgtccgcg ctgggcaaca agttcgcgaa tcgctgcatc ttcccgctca
      601 agatccggag acacggactg ctctttcatc cagagttcct gaccttcaag aaccgacacg
      661 agagcatctc cctcaaggga acgataacgg aaaggatacc tccggacaaa ctggacgaaa
      721 tgacttcgtt ttcgggaaaa acggttccct ttcaactcga cgagctcctg aacagagtgg
      781 aggtactccc actgggagcg gtcttctcct acggtcactc ctccccggaa gagttctctc
      841 cagagacgcc ccaggccgtc gggaacataa aaaaagactc ttccctcagg aaaaagacgg
      901 ggaagcacct ttttccattc aatagcgccc cacggtccca caggagccca cagcgcgtcg
      961 ttccgtgtcc ggatccagca gacgccttct tcgaaggccc attcaaagcc atagatgttt
     1021 cgccacgccc agagacttcc gaaagaatac cacgcggatt tccgcggaga ctgcgagtag
     1081 agctgtctgt attcatccgc tctctccagg gagatcgatg aaaaagaaaa cggcatgaaa
     1141 acatcttcct ttcactgcta cgccaaagaa ttccggagac actccggaac cctttttcag
     1201 acatcgcgta ttgtaccata ttgaaacgcg gcggaccccc gcttcttctg aaaagggggt
     1261 ccgccgcagt tttttcagta aaaatgccga tgggggaaat acttacccca gacccagcat
     1321 tctcccgccc tgatagacca gtaccgcgcc gccgtagcct atcagcgtcg tataggcgat
     1381 catgaagagt gtccaggccc agcttccggt ctccctgcgg aag
//
LOCUS       DAOTIL010000811         1420 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminobacteriaceae bacterium isolate AMR_MDS_3026
            NODE_108806_length_1420_cov_3.983150, whole genome shotgun
            sequence.
ACCESSION   DAOTIL010000811 DAOTIL010000000
VERSION     DAOTIL010000811.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868050
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminobacteriaceae bacterium (wastewater metagenome)
  ORGANISM  Aminobacteriaceae bacterium
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae.
REFERENCE   1  (bases 1 to 1420)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1420)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:19:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,224
            CDSs (total)                      :: 3,198
            Genes (coding)                    :: 3,184
            CDSs (with protein)               :: 3,184
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1420
                     /organism="Aminobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_108806_length_1420_cov_3.983150"
                     /isolate="AMR_MDS_3026"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:3034609"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(10..1056)
                     /locus_tag="P5201_13490"
     CDS             complement(10..1056)
                     /locus_tag="P5201_13490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006300080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HRV99605.1"
                     /translation="MNHAVELLSNLVAIPSLSGREEHACAFLADVLPAAGWERVEIDG
                     AGSVVASRGSGSRELILMGHIDTVPGGPAYALDGDILRGRGSVDAKGPLCSFAVAGGR
                     APIAPDWKLTLIAATGEEADSRGALHRIPKHAPAACIIGEPSGADGVTIGYRGSLRIA
                     LSAEDGGAHRSGDAGPITAVVRAAADILSLVESADSPELPVIQRPSGAVVSMHGEENG
                     RRLGRSDLDIRLPERSDPQQWIERIVELAGRRGVHARTLAVTPAHVADKNNPAARALR
                     LAVRKNGLSPRLLAKGGTADFNLAAAWNCPLAAYGPGDSKLDHTSEEHITLSEYLTSL
                     DILTDAIPMIMRSA"
     gene            complement(1053..>1420)
                     /locus_tag="P5201_13495"
     CDS             complement(1053..>1420)
                     /locus_tag="P5201_13495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014806845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="aminotransferase class III-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HRV99606.1"
                     /translation="SGHGSTYGGNPLSLAVANASLKLLDDEGYPEKARAGGEFFRTLL
                     AEIDSPLISEIRGMGLLNGVELTVKAIPVVKRMQEMGVLALPAGPSVVRFLPPFPAGD
                     ADFLKAASVFAEALKEEGR"
BASE COUNT          215 a          461 c          512 g          232 t
ORIGIN      
        1 ggagcgcgtt caggcgctcc tcatgatcat cggaatggcg tccgtcagaa tgtcgaggga
       61 ggtcaggtat tcggagagcg taatatgttc ctcggaggtg tggtcgagct tgctgtcgcc
      121 ggggccgtag gcggccaggg ggcagttcca cgcggcggcg aggttgaagt cggcggtgcc
      181 gccttttgcg aggagcctgg gactcaggcc gttcttccgg acggcgaggc gcagagcgcg
      241 ggccgccgga ttgttcttgt cggcgacgtg cgcgggagtg acggcgagag tacgggcatg
      301 tacgccccgg cgtccggcga gttcgacgat gcgttcgatc cactgctgcg gatcgcttct
      361 ctccgggagg cggatgtcga ggtcgctcct gccaagccgg cggccgttct cctcgccgtg
      421 catggagacg acggcgcccg aaggccgttg aatgaccggc aactccgggg agtcggcgga
      481 ctccactaac gagagtatat ccgccgccgc ccggacgacc gcggttatgg ggcccgcgtc
      541 gccgctccgg tgcgcgcccc cgtcctctgc cgagagcgcg atgcggaggc tccccctgta
      601 gccgatggtg acgccgtccg ccccggaggg ctcgccgata atgcaggcgg caggggcgtg
      661 tttggggatg cggtggagcg cgcctctgga gtcggcctcc tcgccggttg cggcgatgag
      721 cgtgagcttc cagtcgggag cgatcggcgc gcgtccgcct gcgacggcga aggagcagag
      781 cggccccttc gcgtcgacgc tcccgcggcc gcggagaata tccccgtcga gcgcgtaggc
      841 cgggccgccg gggacggtgt cgatatgccc catgaggatg agttcgcggc ttcccgatcc
      901 cctggaggcg acgacgctcc ccgcgccgtc gatctccacg cgctcccatc cggccgccgg
      961 aagcacgtcg gcaagaaagg cgcacgcgtg ctcttcccgc ccgctcagac tcgggatggc
     1021 gacaaggttc gacagcagtt cgacagcatg gttcaccgcc cttcctcctt cagcgcttcg
     1081 gcgaagacgg acgcggcctt caggaagtcc gcgtcgcccg cggggaaggg gggaagaaac
     1141 cggacgacgg acggcccggc agggagggcg agcacgccca tctcctgcat ccgcttcacc
     1201 acgggaatgg ccttgacggt gagctccacg ccgttcagca gccccatgcc ccggatttcc
     1261 gaaatcaacg gcgagtcgat ctccgccagc aacgttctga agaattctcc gccggcacgc
     1321 gctttttcag ggtagccttc gtcgtcaagc agcttcagcg aagcgttcgc cacggcgagg
     1381 gagagcgggt tgccgccgta cgtggagccg tgtccggagg
//
LOCUS       DAOTIL010000812         1419 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminobacteriaceae bacterium isolate AMR_MDS_3026
            NODE_108995_length_1419_cov_1.815982, whole genome shotgun
            sequence.
ACCESSION   DAOTIL010000812 DAOTIL010000000
VERSION     DAOTIL010000812.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868050
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminobacteriaceae bacterium (wastewater metagenome)
  ORGANISM  Aminobacteriaceae bacterium
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae.
REFERENCE   1  (bases 1 to 1419)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1419)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:19:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,224
            CDSs (total)                      :: 3,198
            Genes (coding)                    :: 3,184
            CDSs (with protein)               :: 3,184
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1419
                     /organism="Aminobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_108995_length_1419_cov_1.815982"
                     /isolate="AMR_MDS_3026"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:3034609"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..905
                     /locus_tag="P5201_13500"
     CDS             <1..905
                     /locus_tag="P5201_13500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005661165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="CaiB/BaiF CoA-transferase family protein"
                     /protein_id="HRV99607.1"
                     /translation="RPGTMEKLGLGYEELKRINPAIIYAAISGFGHTGRYSQRPGYDI
                     ISQAMSGLMSTTGWPDGEPTRTGTAMGDVLGGLSCAIGILSAVFNRLSTGLGQKVDVA
                     LVDSAVASLEIINMIYLVEGRIPQRIGNRYESTYPYDSFRASDGSLVIGVANDKLWAQ
                     LCEAMDLQHLINDPRFTRVRDRVANHAELKPLIEEWTTRFTVDEVCEKVSAAGVPCAP
                     ILNIEQVVNAPHIGGDREMFVDMEHPVAGKLKVTGSHIKLSDTPAGVRTPSPALGQNN
                     EEILGSLLSMTSEAIAALRAEGAV"
     gene            910..>1419
                     /locus_tag="P5201_13505"
     CDS             910..>1419
                     /locus_tag="P5201_13505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005661166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylglutaryl-CoA lyase"
                     /protein_id="HRV99608.1"
                     /translation="MNFSLPERIEIMEVCPRDGFQSVKEFIPTEGKIAILDALAGTGI
                     STMEITSFVSPKAIPQMADAADVTAHFNTKWRGKITSVALVPNLRGAESALAAGVDWM
                     NFVISASVDHNMANTRRTVEESIEELKKVCALKGETKLRLSVATSFACPFAGDVPPEK
                     VVRVVDACLE"
BASE COUNT          295 a          457 c          427 g          240 t
ORIGIN      
        1 tccgcccggg cacgatggaa aagctcggtt tggggtacga agagctgaaa agaattaacc
       61 cggcgatcat ctacgccgct atttccggct tcggccacac tgggcgctac agccagcggc
      121 cggggtacga catcatctcc caggcaatga gcggcctcat gagcaccacc ggctggcccg
      181 acggcgaacc gacccgcaca ggaacagcca tgggcgacgt attgggcggg ctctcctgcg
      241 ccatcggcat tctttcggca gtattcaacc gcctctccac aggccttgga cagaaggtcg
      301 acgtggcgct ggtcgactcg gcggtggcaa gcctggagat catcaacatg atctacctcg
      361 tcgaggggcg gataccgcaa cgtatcggca accgctacga gtccacctat ccgtacgact
      421 ccttccgcgc ctccgacggc agcctcgtca tcggcgtggc caacgacaaa ctctgggcgc
      481 agctctgcga ggccatggac ctgcagcacc tgatcaacga cccccgcttc actcgggtgc
      541 gcgaccgcgt ggcgaaccac gccgagctga agccgctgat cgaggagtgg accactcgct
      601 tcacagtgga cgaggtctgc gagaaggtct ccgcggcggg cgtcccgtgc gcgccgattc
      661 tgaatatcga acaggtcgtg aacgccccgc atatcggcgg cgacagggag atgttcgtcg
      721 acatggagca ccccgtcgca ggaaaactga aggtcaccgg aagccacatc aagctttccg
      781 atacccctgc cggagtacgt accccctccc ccgccctggg acagaacaac gaggagatcc
      841 tgggatcgct tctcagcatg acctccgagg ccatcgcggc gcttcgagca gagggagcgg
      901 tgtagcctca tgaacttctc tctgcccgaa cgtatcgaga tcatggaggt ctgcccccgg
      961 gacggcttcc agagcgtaaa ggagttcatc cccaccgagg gaaagatcgc gattctcgac
     1021 gcgctcgccg ggacgggcat ctccacaatg gagattacct cttttgtgag cccgaaggct
     1081 attccgcaga tggcggacgc tgcggacgtg acggcgcact tcaacacaaa gtggcggggg
     1141 aagatcacct ccgtcgccct cgtaccgaat ctccggggcg ccgagtcggc cctcgcggcg
     1201 ggcgtcgact ggatgaactt cgttatctcg gcgagcgtcg atcataacat ggcgaacact
     1261 cgaagaacgg ttgaagaatc catcgaagag ctgaagaaag tctgcgcgct gaagggagag
     1321 acaaagctcc gcctctcggt ggcgacctcc ttcgcctgcc ccttcgccgg agacgtcccc
     1381 ccagaaaaag tcgtccgcgt ggtggacgct tgccttgaa
//
LOCUS       DAOTIL010000813         1418 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminobacteriaceae bacterium isolate AMR_MDS_3026
            NODE_109029_length_1418_cov_3.908291, whole genome shotgun
            sequence.
ACCESSION   DAOTIL010000813 DAOTIL010000000
VERSION     DAOTIL010000813.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868050
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminobacteriaceae bacterium (wastewater metagenome)
  ORGANISM  Aminobacteriaceae bacterium
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae.
REFERENCE   1  (bases 1 to 1418)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1418)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:19:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,224
            CDSs (total)                      :: 3,198
            Genes (coding)                    :: 3,184
            CDSs (with protein)               :: 3,184
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1418
                     /organism="Aminobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_109029_length_1418_cov_3.908291"
                     /isolate="AMR_MDS_3026"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:3034609"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(78..953)
                     /locus_tag="P5201_13510"
     CDS             complement(78..953)
                     /locus_tag="P5201_13510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009202417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase family protein subunit M"
                     /protein_id="HRV99609.1"
                     /translation="MLAFEMERPRSLAAAVEILGKYGSKAMIKAGGTDVLVWMRKHAV
                     NPEMLVDLSEIPELGGISFYPHKGIKIGAMCTVNQVAEHEDARRYYGALTDACLSHSD
                     PLIRNKATVLGNVCASVPSGDMIPAFAAYEAVLHVYGPEGERDILFADFITGPRKNVL
                     VPGEIVVSATLPLPAGQSTGCYIKLGRRNALDLAQVGVACVAIDGEKGREYRLAYGAV
                     APRPVRAAEAEDILAGQTSPDAALLEKAAKAAAAAVNPITDVRASREYRFSMVEELTK
                     RAVSLCAERFQGGNA"
     gene            complement(1050..>1418)
                     /locus_tag="P5201_13515"
     CDS             complement(1050..>1418)
                     /locus_tag="P5201_13515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006301720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclase family protein"
                     /protein_id="HRV99610.1"
                     /translation="HPGPTREFATWALKKKIKWIGVDCGSADHPMNTKIREWMPVQAR
                     QADAHLKKKYGKGLDDFFPEEDYQVMHIALFPSNLIHAECVAGDIDQLSGKRVTIGCF
                     PWRFVGGESCISRIVAFAEE"
BASE COUNT          261 a          439 c          437 g          281 t
ORIGIN      
        1 ccgcagcagc gtggtgcggg gctcgaccga aagagtcgtc tgtctgccgt tcaaaatgaa
       61 atgaacatcg cgcttcatca tgcgttccct ccctggaatc tctccgcgca aagagagacg
      121 gcccgtttcg tcagttcctc gaccatggag aagcggtact cacggctcgc ccggacgtcg
      181 gtgataggat tcaccgcggc cgccgcggct ttcgcggcct tttccagaag cgccgcgtcg
      241 ggggacgtct gtccggcaag gatatcctcg gcctcggcgg cgcgcacggg gcgcggagcc
      301 accgctccgt acgccagccg gtactctctg cccttctcgc cgtcgatcgc gacgcaggcc
      361 acgccgacct gcgcgaggtc gagcgcgttc cttcgtccga gcttgatgta gcaccccgtc
      421 gactggcctg ccggcagagg gagcgtcgcg gagacgacga tctctccggg aaccagaacg
      481 ttctttctgg gcccggtgat aaaatcggcg aaaagaatat cgcgctcgcc ttcggggccg
      541 tagacgtgca ggacggcttc gtaggcggcg aacgcaggaa tcatatcccc ggagggaacg
      601 gaggcgcaga cgttccccag caccgtcgcc ttgttccgga tcaacgggtc cgagtgggag
      661 aggcatgcgt ccgtcagggc gccgtagtat cttcgggcgt cctcgtgctc ggcgacctgg
      721 ttgacagtgc acatcgcgcc gattttgatt cccttgtgag gatagaagga aataccgccg
      781 agttcaggaa tctccgagag atccacgagc atctccgggt tgacggcatg tttgcgcatc
      841 cacacaagca cgtccgttcc acccgccttg atcatcgcct tggaaccgta tttccccaga
      901 atttcgacag ccgcggccag cgatctgggg cgttccattt caaatgccag cattgacata
      961 tcaccccttc ggtgagaaat cagtatgtga agagggcgtc cccgaaccct ctgaaaacca
     1021 tgaggaggag gacgcccgtc gtaacggagt tattcttcgg caaacgcgac gatgcgcgag
     1081 atgcaggact cgccgcccac gaatctccac gggaagcagc cgatggtgac ccgtttgccg
     1141 ctgagctggt cgatgtcgcc cgcgacgcac tcggcgtgga tcagattgct ggggaagagg
     1201 gcgatatgca tcacctggta gtcctcttca gggaagaaat cgtcgagccc cttgccgtac
     1261 ttcttcttca ggtgcgcgtc ggcctgtctg gcctgaaccg gcatccactc gcggatcttc
     1321 gtgttcatcg gatggtcggc ggaaccgcag tcgacgccga tccacttgat cttcttcttg
     1381 agcgcccagg tggcgaactc tctggtgggt ccggggtg
//
LOCUS       DAOTIL010000814         1417 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminobacteriaceae bacterium isolate AMR_MDS_3026
            NODE_109157_length_1417_cov_3.214391, whole genome shotgun
            sequence.
ACCESSION   DAOTIL010000814 DAOTIL010000000
VERSION     DAOTIL010000814.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868050
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminobacteriaceae bacterium (wastewater metagenome)
  ORGANISM  Aminobacteriaceae bacterium
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae.
REFERENCE   1  (bases 1 to 1417)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1417)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:19:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,224
            CDSs (total)                      :: 3,198
            Genes (coding)                    :: 3,184
            CDSs (with protein)               :: 3,184
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1417
                     /organism="Aminobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_109157_length_1417_cov_3.214391"
                     /isolate="AMR_MDS_3026"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:3034609"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1040
                     /gene="thrS"
                     /locus_tag="P5201_13520"
     CDS             <1..1040
                     /gene="thrS"
                     /locus_tag="P5201_13520"
                     /EC_number="6.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009201222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004829 - threonine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006435 - threonyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="threonine--tRNA ligase"
                     /protein_id="HRV99611.1"
                     /translation="IRTPLILERSLWIQSGHWDHYRDNMYFTEIDERPFAIKPMNCPG
                     GMLVYKSQIRSYRDLPLRMAELGVVHRHERSGVLHGLMRVRCFTQDDAHLYCRPDQVK
                     DEIKGIMDLCRFIYKDVFGFKYTMELSTRPDNSMGSEEQWKIAETALQQALEETGSEY
                     KLNPGDGAFYGPKIDFHLEDCIGRTWQCGTIQLDFQMPEKFDLKYIGDDGKEHRPVML
                     HRTVLGSLERFFGILIENFAGAFPYWIAPLQARVLPVSDEFVEYGKSVEQTLRSWGVR
                     VETDERDEKLGKKIRDAQTQKVPYMIVIGEKERESGTVAPRERAKGDLGSMSLEQFRK
                     VLDEEFNPLKQ"
     gene            1230..>1417
                     /locus_tag="P5201_13525"
     CDS             1230..>1417
                     /locus_tag="P5201_13525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005660523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C4-dicarboxylate ABC transporter
                     substrate-binding protein"
                     /protein_id="HRV99612.1"
                     /translation="MNKKRIAVLCVALFILAFAGAGFASTKFVTIVTGSTGGTYYPIG
                     TILANHFNTALMEKGYKWS"
BASE COUNT          334 a          385 c          417 g          281 t
ORIGIN      
        1 agatccgcac cccgcttatc ctcgaacgct cgctctggat tcagtccggc cactgggatc
       61 actacagaga caacatgtac ttcaccgaga tcgacgagcg tcccttcgct atcaaaccta
      121 tgaactgccc cggaggcatg ctcgtctaca agagtcagat ccggagctac cgcgacctgc
      181 cgctcaggat ggcggagctc ggcgtggtgc accgtcacga gcggagcggc gtgctccatg
      241 gactgatgcg cgtccgatgc ttcacacagg acgacgcgca cctctattgc cgccccgacc
      301 aggtgaagga cgagatcaaa ggcatcatgg atctctgccg cttcatctac aaggacgtct
      361 tcggcttcaa atacaccatg gagctctcga cgcgcccgga caattcgatg ggctctgaag
      421 agcagtggaa gatcgccgag acggccctgc agcaggcgct tgaggagacc ggatcggagt
      481 acaagctcaa cccgggcgac ggcgcgttct acggcccgaa gatcgacttc caccttgagg
      541 attgcatcgg caggacgtgg cagtgcggta ccatccagct cgatttccag atgcccgaga
      601 agttcgatct gaagtatatc ggcgacgacg gaaaggaaca ccgccccgtc atgctccaca
      661 gaacggtcct cggcagcctg gagcgcttct tcggcatcct catcgagaac ttcgccggcg
      721 ccttcccgta ctggatcgcg cctctgcagg cccgggtgct ccccgtctcc gacgaattcg
      781 tcgagtacgg aaagagcgtg gagcagacgc ttcgttcatg gggcgtacgc gttgagaccg
      841 acgaacgcga cgagaagctc ggcaagaaga tccgggacgc gcagacgcag aaggtgccgt
      901 acatgatcgt catcggcgaa aaagaacgcg agtccggtac ggtcgctcca agagagcggg
      961 cgaagggcga tcttggttcc atgtcgctgg agcagttcag gaaagtgctt gacgaggagt
     1021 tcaatccgct gaagcaataa ttttggttca gcataatctt ccttgaggaa agaatgttca
     1081 gaacaggagt cgcttgaaag ccgaagcgac tcctgttaca atcaacgagt gttttgttaa
     1141 cagtgagtga agatagtatt tcggggggtg aaatcaccga agacgtgggg tgcgatgaag
     1201 tacacaatta cgagagaggt ggatgaaaaa tgaacaaaaa gagaatcgct gttttgtgtg
     1261 tcgcgctgtt tattctggcc tttgcaggag ccggatttgc ttcgacgaag tttgtcacca
     1321 tcgttaccgg ttccacgggc ggaacgtact atcccatcgg tacgatcctc gccaatcatt
     1381 tcaacacggc gttgatggag aaggggtaca agtggtc
//
LOCUS       DAOTIL010000815         1415 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminobacteriaceae bacterium isolate AMR_MDS_3026
            NODE_109427_length_1415_cov_2.578676, whole genome shotgun
            sequence.
ACCESSION   DAOTIL010000815 DAOTIL010000000
VERSION     DAOTIL010000815.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868050
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminobacteriaceae bacterium (wastewater metagenome)
  ORGANISM  Aminobacteriaceae bacterium
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae.
REFERENCE   1  (bases 1 to 1415)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1415)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:19:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,224
            CDSs (total)                      :: 3,198
            Genes (coding)                    :: 3,184
            CDSs (with protein)               :: 3,184
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1415
                     /organism="Aminobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_109427_length_1415_cov_2.578676"
                     /isolate="AMR_MDS_3026"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:3034609"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>1415
                     /locus_tag="P5201_13530"
     CDS             <1..>1415
                     /locus_tag="P5201_13530"
                     /inference="COORDINATES: protein motif:HMM:TIGR03491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TM0106 family RecB-like putative nuclease"
                     /protein_id="HRV99613.1"
                     /translation="RSIKPVFPLQLCLYSEMLAPLQGEIPERFHIALGSGETRSFRIE
                     RFLHLYRALRERFLAFQETFDPSAPPHPGDSPAHGGWSGTAGRILERLDSLARVANIA
                     GSQRKKLEKAGIVRMKDLAETREERVPGLSPDVFARLREQASLQIASEGKERPEYRIV
                     PSADGEELLPPASPGDVYFDIEGYPHAEGGLEYLWGAVGTDTPAPSGEGEVVFYDWWA
                     HDAAGEKAAFEQFIDWVRARRTLHPGMHVYHYAPYETTAAKRLAAKYATREEEVDILL
                     REGVFVDLYRVVRRALLVGTPGYSLKDVEHLYREKRSGDVATGGASVVAYHEWTVSGE
                     SGDWRKSPLLRHIRDYNEEDCRSTMDLGLWLREHLKEERVAPLDSPGESAVPSEREKT
                     VRNEKNERRKELRRSLPGVLLEDVRGHADRPAAELLAGLVGYHWREAKPVFWRMYDRL
                     ARTGAELADDPDCLGMLRIAG"
BASE COUNT          245 a          473 c          464 g          233 t
ORIGIN      
        1 cgttcgatca aaccggtctt cccccttcag ctctgcctct actccgagat gctcgctccc
       61 ctccaggggg agattcccga aaggtttcat atcgccctcg gttccggcga gacgagaagc
      121 ttccggatcg agcgctttct ccacctctac cgcgctcttc gcgagcgctt tctggcattt
      181 caggagacgt tcgatccgtc ggcgccgccg catccgggag actccccggc gcacggtgga
      241 tggagcggga cggcggggcg cattctcgag cggctcgact cgctcgcccg cgtcgccaac
      301 atcgccggat cgcagcggaa gaagctggag aaggccggaa tcgtgcgaat gaaggatctg
      361 gccgaaactc gggaagagcg cgtccccggt ctctcccccg acgtcttcgc ccgcctccgc
      421 gagcaggctt ccttgcagat cgcctccgaa ggaaaggagc ggccggagta tcgcattgtt
      481 ccgagcgccg acggggagga gcttcttcct cccgcctccc ccggcgacgt ctatttcgac
      541 atcgaaggct acccgcacgc cgaaggcggg ctcgaatacc tctggggcgc cgtcggcacg
      601 gacacgccgg ccccttcggg ggagggtgag gtcgtgttct acgactggtg ggcgcacgac
      661 gccgccggag agaaggccgc cttcgaacag ttcatcgact gggtccgcgc gcgcaggacg
      721 ctccaccccg gaatgcacgt ctaccactac gccccgtacg agaccacggc ggccaagcga
      781 cttgccgcga agtacgccac ccgggaggag gaagtcgaca tcctcctccg cgaaggagtg
      841 ttcgtcgatc tctaccgcgt cgtccggaga gcgctgctgg tgggaactcc gggctactcc
      901 ctgaaggatg tggagcacct ctaccgggag aagcgctccg gagatgtggc gacgggcggc
      961 gcttccgtgg tcgcctacca cgagtggact gtaagcggcg agtctggaga ctggaggaaa
     1021 tccccgctgc ttcggcacat tcgcgactac aacgaggagg actgccgctc cacgatggat
     1081 ctcgggctct ggctccgcga gcatttgaag gaggagcgcg tcgctccgct ggactccccc
     1141 ggggagagcg ccgttccttc ggagagggag aagactgtgc ggaacgagaa gaacgaacga
     1201 cggaaggagc ttcgccggag tcttcccggg gtcctcctcg aagacgtccg cggccacgcc
     1261 gaccgcccgg ctgcggaact tctcgccgga ctcgtgggct accactggcg cgaggcaaaa
     1321 cccgtcttct ggcgcatgta cgacaggctg gcgcgcaccg gcgccgaact ggcggacgat
     1381 cccgactgcc tcggtatgct tcgcatcgcc gggga
//
LOCUS       DAOTIL010000816         1414 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminobacteriaceae bacterium isolate AMR_MDS_3026
            NODE_109542_length_1414_cov_2.353937, whole genome shotgun
            sequence.
ACCESSION   DAOTIL010000816 DAOTIL010000000
VERSION     DAOTIL010000816.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868050
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminobacteriaceae bacterium (wastewater metagenome)
  ORGANISM  Aminobacteriaceae bacterium
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae.
REFERENCE   1  (bases 1 to 1414)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1414)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:19:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,224
            CDSs (total)                      :: 3,198
            Genes (coding)                    :: 3,184
            CDSs (with protein)               :: 3,184
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1414
                     /organism="Aminobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_109542_length_1414_cov_2.353937"
                     /isolate="AMR_MDS_3026"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:3034609"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..457)
                     /locus_tag="P5201_13535"
     CDS             complement(<1..457)
                     /locus_tag="P5201_13535"
                     /inference="COORDINATES: protein motif:HMM:NF012955.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HRV99614.1"
                     /translation="MLDSLEIITLMEDSVPMNGHLVAEHGLSFFIKGTKDGKEVRGLL
                     DVGQTAEFLAHNMKELGVCPQSVDFVVLSHCHYDHTGGIAKFVESTGKHDFPVIANPA
                     LFRPHFVMEPVVAAKGVRTGDEREAIERADGMLLLSGAPFPLADGLSTTG"
     gene            550..>1414
                     /locus_tag="P5201_13540"
     CDS             550..>1414
                     /locus_tag="P5201_13540"
                     /EC_number="6.3.1.20"
                     /inference="COORDINATES: protein motif:HMM:TIGR00545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0017118 - lipoyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoate--protein ligase"
                     /protein_id="HRV99615.1"
                     /translation="MYFIEGQSHDPAYNLALEEYLYRSVNEGHPGYFLLWQNAPSIIV
                     GRFQNTAQEVNRPFVEERGIHVVRRISGGGAVYHDLGNVNYSFIVPNDEGTPFDFARH
                     ARPVIDALAKLGVEAESGGRNDLLIDGRKFSGSAQHMDRRRLLHHGTLLFDSDLSILE
                     QALSVDEEKFTSKGFKSVRSRVTNILPMLTEPISVEEFIDALRGALSGTETYSLDETQ
                     LSEIRRLRDEKYATWEWNWGASPEFTERKARRFPWGKVEALLNVKEGVIRQARFYGDY
                     FGTENREEIERL"
BASE COUNT          322 a          425 c          411 g          256 t
ORIGIN      
        1 ctccagtggt cgacagtccg tcggcaagag gaaacggcgc gcctgagagc aacagcattc
       61 cgtcggccct ctcgatagct tcgcgctcgt cgccggtacg gacgcccttc gccgccacaa
      121 ccggttccat tacaaagtgc ggacgaaaga gcgcaggatt cgcgatcacc gggaagtcat
      181 gcttccctgt ggactcaacg aacttcgcga tacctccggt gtggtcgtag tgacagtgtg
      241 agagaacaac aaagtcgacc gactgcgggc aaaccccaag ctctttcatg ttgtgcgcca
      301 ggaactccgc agtctgcccc acgtcgagga gacctcggac ctcctttccg tctttcgtcc
      361 ccttgataaa gaacgaaagg ccgtgctcgg cgacaaggtg accgttcata ggcactgagt
      421 cttccatcaa tgtgataatc tcaagtgaat cgagcatatc gctccacctc cggatacagg
      481 atattttgta taatgaactg aaaccgccga ctttgcaaga ggcgccggaa cgagaggaga
      541 gaaatcgaaa tgtactttat cgaaggacaa tcgcacgacc ccgcgtacaa cctcgcgctc
      601 gaggagtatc tctacaggag cgtcaacgaa ggacaccccg gctacttcct gctgtggcaa
      661 aacgctccct cgatcatcgt ggggcgcttc cagaacacgg ctcaggaggt gaaccgcccc
      721 ttcgtcgagg agcgcggcat tcacgtcgtc agacggatat ccggaggagg cgcggtctat
      781 cacgacctcg ggaacgtgaa ctactcgttc atcgtcccca acgacgaggg aacccccttc
      841 gactttgcgc gccatgcccg gccagtaatc gacgcgctcg caaaactcgg cgtcgaagct
      901 gagagcggcg gcaggaatga tcttttaatc gacgggcgaa agttctccgg aagcgcccag
      961 cacatggacc ggcgcagact gctccatcac gggacgctgc tcttcgattc ggacctctcg
     1021 atccttgaac aggccctcag cgtggacgag gaaaagttca cctccaaggg gttcaagtcg
     1081 gtccggagcc gagtgaccaa catcctcccc atgctgacgg agcccatctc cgtggaggag
     1141 ttcatcgacg cgcttcgggg cgctctctcc gggaccgaaa catattcgct cgacgagaca
     1201 cagctttcgg aaatccgccg tcttcgggac gagaagtacg cgacgtggga gtggaactgg
     1261 ggggcgtccc ccgagttcac cgagcgcaag gcccgccgct tcccgtgggg gaaggttgag
     1321 gcgctgctga acgtgaagga gggcgtcata cggcaagcgc gattctacgg cgactacttc
     1381 ggcacggaga acagggaaga gatcgaaagg ctgc
//
LOCUS       DAOTIL010000817         1412 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminobacteriaceae bacterium isolate AMR_MDS_3026
            NODE_109686_length_1412_cov_4.810612, whole genome shotgun
            sequence.
ACCESSION   DAOTIL010000817 DAOTIL010000000
VERSION     DAOTIL010000817.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868050
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminobacteriaceae bacterium (wastewater metagenome)
  ORGANISM  Aminobacteriaceae bacterium
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae.
REFERENCE   1  (bases 1 to 1412)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1412)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:19:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,224
            CDSs (total)                      :: 3,198
            Genes (coding)                    :: 3,184
            CDSs (with protein)               :: 3,184
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1412
                     /organism="Aminobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_109686_length_1412_cov_4.810612"
                     /isolate="AMR_MDS_3026"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:3034609"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..351)
                     /locus_tag="P5201_13545"
     CDS             complement(<1..351)
                     /locus_tag="P5201_13545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009164437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HRV99616.1"
                     /translation="MNGPLLEVSDLKVVYKTYEGTVAAVNGLDFSLHRGKTLGLVGES
                     GAGKTTTALSILGLVPSPPGVIESGRIVFDGRDLLSLPEREMKKIRGNRISMIFQDPM
                     TSLNPVVTVGVQISE"
     gene            complement(371..1276)
                     /locus_tag="P5201_13550"
     CDS             complement(371..1276)
                     /locus_tag="P5201_13550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009164791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HRV99617.1"
                     /translation="MNSERQSIVTLSAADGKTREKRRSPWWEVWRRLRRNKAALVGMT
                     VVVLLVVVAVLAPVLAPYDPNQTNMTQRLKAPSSAHPLGTDNFGRDMLSRVIYGSRIS
                     LYVGFVAVGIGAIFGGILGAICGYYGGRLDNFIMRCMDVLLAIPQIILAIAIVGALGT
                     SLLNLMIAVGISQLPRYARVVRASALTVRGQEFVEAAKAVGASDARIIVENILPNCMA
                     PIIVQSTLGVAQAILSAAALSFLGLGIQPPTAEWGSMLSSGRQFLRQAPYLTFFPGLA
                     ISIVVLALNIFGDGLRDSLDPKLRQ"
     gene            complement(1296..>1412)
                     /locus_tag="P5201_13555"
     CDS             complement(1296..>1412)
                     /locus_tag="P5201_13555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005659868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HRV99618.1"
                     /translation="VQGGVLFFALCVGIVNLLVDLLYAVLDPRIKAQYRAAK"
BASE COUNT          258 a          453 c          463 g          238 t
ORIGIN      
        1 ctcgctgatc tggacgccga ccgtgaccac ggggttcagc gaggtcatgg gatcctggaa
       61 tatcatcgag attctgttgc cgcgaatttt tttcatctcc cgctccggga gcgagagcag
      121 atcgcgcccg tcgaagacga tgcgcccgct ttcgataact ccgggagggg aggggacgag
      181 cccgagtatg gagagggcgg tcgtcgtctt tcccgcgccg ctctccccga cgagcccgag
      241 cgtcttcccg cggtgaaggg agaagtcgag cccgttcacc gcggccaccg tgccttcgta
      301 cgtcttgtag accactttga gatccgaaac ttccaacaga ggaccgttca tgcgaaaacc
      361 tcccgacgga ctactggcga agcttgggat cgagtgaatc ccgaagtccg tcgccgaata
      421 tgttgagggc cagcaccacg atcgagatgg ccagcccggg gaagaacgtg agatagggcg
      481 cctggcgcag gaactgccgt ccggagctga gcatggagcc ccactccgcc gtgggcggct
      541 ggattcccag cccgaggaag gagagggccg ccgccgacag gatggcctgg gccacgccga
      601 gggtgctctg gacgatgatg ggggccatgc agttcggcag gatgttctcg acgatgatcc
      661 gcgcgtcgct cgcgccgacg gccttggccg cctcgacgaa ctcctgtccc ctgaccgtga
      721 gcgccgacgc ccgcaccacc cgcgcgtagc ggggaagctg gctgatgccc accgcgatca
      781 tcaggttcaa caggctggtc cccagcgcgc cgacgatggc gatggcgagt atgatctgcg
      841 ggatggcgag gaggacgtcc atgcagcgca tgatgaagtt gtccagtctg ccgccgtagt
      901 agccgcagat ggcgccgaga atgccgccga agatcgcgcc gatgcccacc gccacgaagc
      961 cgacgtagag cgaaatccgg ctgccgtaga taacgcggct gagcatgtcg cgcccgaagt
     1021 tgtcggttcc cagcgggtgc gccgacgagg gagccttgag gcgctgcgtc atgttcgtct
     1081 ggttcggatc gtacggcgcg aggacggggg ccagcacggc cacgaccacc agcaggacga
     1141 ccacggtcat gcccaccagc gccgccttgt tccgccgcag acgccgccag acctcccacc
     1201 acgggctgcg gcgcttttcc cgcgtctttc cgtcggcggc ggagagggta acgatcgatt
     1261 gtctttcact gttcatgagt gttcgctcct ctccgtcatt tcgcggccct gtactgcgcc
     1321 ttgatccggg gatcgagcac ggcgtacagc aggtccacga gcagattcac tatgccgacg
     1381 cacaaggcga agaagagcac gcctccctgc ac
//
LOCUS       DAOTIL010000818         1412 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminobacteriaceae bacterium isolate AMR_MDS_3026
            NODE_109795_length_1412_cov_1.450258, whole genome shotgun
            sequence.
ACCESSION   DAOTIL010000818 DAOTIL010000000
VERSION     DAOTIL010000818.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868050
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminobacteriaceae bacterium (wastewater metagenome)
  ORGANISM  Aminobacteriaceae bacterium
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae.
REFERENCE   1  (bases 1 to 1412)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1412)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:19:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,224
            CDSs (total)                      :: 3,198
            Genes (coding)                    :: 3,184
            CDSs (with protein)               :: 3,184
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1412
                     /organism="Aminobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_109795_length_1412_cov_1.450258"
                     /isolate="AMR_MDS_3026"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:3034609"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(34..756)
                     /locus_tag="P5201_13560"
     CDS             complement(34..756)
                     /locus_tag="P5201_13560"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRV99619.1"
                     /translation="MDQIFCDASYKNGVCGVAAATPLTLWNKDKETRKKSVFHKISHL
                     FQSAILGKEGYVVFFSAFPCAGSNEAEIKALGLAIAMAEDILLSESRNGRPVEISSDS
                     LVALGDVLASEEPLFKESEVLRNRIATRKIILSKVKAHAGHQGNEIADEWSKKARRGL
                     ESSTEKEPDTHPEDRTPHSAGDTDKEDSICGFTARLLKETVFPAVITSDGRIVPRSLA
                     FKSGDRNYTELFKERVWGGCWD"
     gene            complement(758..1183)
                     /locus_tag="P5201_13565"
     CDS             complement(758..1183)
                     /locus_tag="P5201_13565"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRV99620.1"
                     /translation="MNWYEKPENQHRLQKIESVVMSRKFPQFSLFKSKERSKYASVGD
                     IYWGGKLRTNFGSVYSVAVVYPENFPYGQIKAYIPELIGVSTPHKYVDGHLCLYSNDH
                     GGGGEGFGKETTAATVVGWTAAWLNAWEVYKRTKEWPGR"
BASE COUNT          261 a          366 c          399 g          386 t
ORIGIN      
        1 cgtatatgaa ggcgacaatg cctacaatga ctatcaatcc cagcaaccgc cccacaccct
       61 ttctttgaag agttccgtat agtttcggtc gccggacttg aacgcgaggc ttctagggac
      121 gatgcgtccg tcgcttgtta tcactgccgg gaaaacggtt tctttcagca ggcgcgcggt
      181 gaagccgcat atcgaatctt ccttgtccgt gtcgccggca gagtgcggcg ttcgatcctc
      241 gggatgggta tccggctcct tttccgtgct ggactccaag cctctccggg cttttttgga
      301 ccattcgtcg gcgatctcgt tcccctgatg tccggcgtgc gcctttacct tgctcaagat
      361 gatctttctg gtggcgatcc ggtttctgag tacttcgctc tctttaaaaa gaggctcttc
      421 ggatgcaagt acgtctccga gcgccacaag gctgtcgctg cttatctcga cgggacgccc
      481 gttcctgctt tccgagagga gtatgtcctc cgccatggcg atggcgaggc cgagagcctt
      541 gatctcggcc tcgttgcttc cagcgcaggg gaaagcggag aaaaagacga catacccttc
      601 ttttcccaat atagctgatt gaaagagatg agagatcttg tggaagacgc tctttttccg
      661 ggtttctttg tctttgttcc agagcgtgag gggagttgcc gccgcaacgc cgcacacgcc
      721 gtttttatac gaggcatcgc agaatatctg gtccatttta ccgtccgggc cattccttcg
      781 tacgcttgta cacttcccac gcgttaagcc atgcggcggt ccagcccacg acggtggctg
      841 ccgtggtctc ctttccgaat ccttctccgc ctcctccgtg gtcgttggag tagagacaga
      901 ggtggccgtc cacatatttg tgaggagtgg agactccgat gagttcggga atataggctt
      961 tgatctgacc gtaggggaag ttttcggggt agacgaccgc tacgctgtag acgctgccga
     1021 agttggtgcg gagcttgccg ccccagtaga tgtcgccgac ggatgcgtat ttgctgcgct
     1081 ctttgctctt gaagagggag aactggggga atttgcggct catgacgacg ctctcgatct
     1141 tctgaagacg gtgctggttc tccggttttt cataccaatt catggcaatt ctcctttcgc
     1201 ttctcgttcc ggaatgcggt attttaacct gcttctctga aatatcgtgt atatgggtga
     1261 atttattcgt tgcggtcgac tttcagggag ctgagaggga gtgtatctga cgggagacgt
     1321 cgaggagtag atgctcttca agtgtgtcga gaaagtggct catggatgtc gttagcgatc
     1381 ttttcaactg gtcttcctgg aagtagcgaa ta
//
LOCUS       DAOTIL010000819         1411 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminobacteriaceae bacterium isolate AMR_MDS_3026
            NODE_109828_length_1411_cov_3.559735, whole genome shotgun
            sequence.
ACCESSION   DAOTIL010000819 DAOTIL010000000
VERSION     DAOTIL010000819.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868050
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminobacteriaceae bacterium (wastewater metagenome)
  ORGANISM  Aminobacteriaceae bacterium
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae.
REFERENCE   1  (bases 1 to 1411)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1411)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:19:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,224
            CDSs (total)                      :: 3,198
            Genes (coding)                    :: 3,184
            CDSs (with protein)               :: 3,184
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1411
                     /organism="Aminobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_109828_length_1411_cov_3.559735"
                     /isolate="AMR_MDS_3026"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:3034609"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            283..1110
                     /locus_tag="P5201_13570"
     CDS             283..1110
                     /locus_tag="P5201_13570"
                     /inference="COORDINATES: protein motif:HMM:NF024803.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HRV99621.1"
                     /translation="MDKLSIGKMAEINRVSVQTLRLYDRLGLLKPQYVDENRYRYYSI
                     KQSARLDMIQNLQLLGMSLRQIREQLDTQDVAVVEKLLEKQKARLDEELLRLSATRRC
                     VERTLINYKRYHSAPRDGMIVVEFIPDRTIYCYDSGINFYDYGLETYEYILRELKGHI
                     ALNALPMAYFCNVGTVLRKPLLLRKEFVSTEVFIFVDDDFQQSPNVETLPSGTYLCMS
                     CDSFHKEKEYAGKLLAHAEENGYEIAGDYICEVIADLPVFHNNERGMFIKLQVPILF"
     gene            1211..>1411
                     /locus_tag="P5201_13575"
     CDS             1211..>1411
                     /locus_tag="P5201_13575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008712900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrodipicolinate reductase"
                     /protein_id="HRV99622.1"
                     /translation="MERKLAVAQFGCGKMSAYTMRYVYEKGAEIVAAFDMNPAVVGKD
                     IGTIMGTEKKGVTVLDAKMADET"
BASE COUNT          336 a          395 c          411 g          269 t
ORIGIN      
        1 gggaaagaac tacctctgcg aagggtacag gatctttttc aaacactttc tggagcagac
       61 ggaaggggtt cttccatagc cgactcggcg ccgttgtttt ttacccgggg agacttcgct
      121 tcgccctatc cgaaacgccg ccgctccgaa gagaatgtgg tatattcttc gggagcggcg
      181 gaactgcgtc gctctccgta gactcggggg ggaaacgttt ccggagcggg cgggagaaaa
      241 gtccgcgagg ttttcagaga gtgcctgcaa aggggttttt cgatggacaa gctctcgata
      301 ggcaagatgg ccgaaatcaa cagggtctcg gtgcagacgc tgcgcctcta cgaccgtctg
      361 gggctgctga aaccgcagta cgtggacgag aaccgctaca gatactacag catcaagcag
      421 tcggcccgtc tggacatgat acagaacctt cagctcctcg gcatgtcgct tcggcagatc
      481 agggagcagc tcgacacgca ggacgtcgcc gtcgtcgaga agctgctcga gaagcagaag
      541 gccagactcg acgaggaact gcttcgtctc tccgccacca gaagatgcgt cgagcgcacg
      601 ctgatcaact acaagcgcta ccactccgcc cctcgggacg gcatgatcgt cgtcgaattc
      661 atccccgacc gcacgatcta ctgctacgac agcggcatca acttctacga ctacggactg
      721 gagacgtacg agtacatcct ccgggagttg aaagggcaca tcgcgctcaa cgctctcccc
      781 atggcctact tctgcaacgt agggaccgtg ctcagaaaac cgcttctgct gaggaaggag
      841 ttcgtctcca ccgaggtgtt catcttcgtc gacgacgact tccagcagtc gccgaacgtc
      901 gaaactctgc cctccgggac gtatctctgc atgtcgtgcg acagcttcca caaggagaag
      961 gagtacgccg gaaaactcct tgcgcacgcc gaggagaacg ggtacgagat cgcgggcgac
     1021 tacatctgcg aggtgatcgc cgatctgccg gttttccaca acaacgagcg aggcatgttc
     1081 atcaagttgc aggttccgat acttttttaa cgcgcacccc ttgactctat accatgtaga
     1141 gaccctatag tgaaaagaag aacacgtgga agaaccacaa cacactctac gttcaggggg
     1201 gtattgcgtt atggaaagga aactcgcggt cgcccagttc ggatgcggaa agatgtccgc
     1261 ctacacgatg cgctacgtct acgaaaaggg cgcggagatc gtcgccgcgt tcgatatgaa
     1321 cccggcagtc gtgggcaagg atatcggtac gatcatggga acggagaaga agggcgtcac
     1381 ggtgctggac gcgaagatgg ccgacgaaac g
//
LOCUS       DAOTIL010000820         1406 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminobacteriaceae bacterium isolate AMR_MDS_3026
            NODE_110396_length_1406_cov_2.991118, whole genome shotgun
            sequence.
ACCESSION   DAOTIL010000820 DAOTIL010000000
VERSION     DAOTIL010000820.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868050
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminobacteriaceae bacterium (wastewater metagenome)
  ORGANISM  Aminobacteriaceae bacterium
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae.
REFERENCE   1  (bases 1 to 1406)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1406)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:19:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,224
            CDSs (total)                      :: 3,198
            Genes (coding)                    :: 3,184
            CDSs (with protein)               :: 3,184
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1406
                     /organism="Aminobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_110396_length_1406_cov_2.991118"
                     /isolate="AMR_MDS_3026"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:3034609"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..255)
                     /locus_tag="P5201_13580"
     CDS             complement(<1..255)
                     /locus_tag="P5201_13580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014162680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HII"
                     /protein_id="HRV99623.1"
                     /translation="MELKEFGRIAGTDEAGRGPLCGPVVAAAVILTETQRDELLALGL
                     ADSKKLSPRKREALFGRMLSLGVLWRAQAASHERIDRMNIL"
     gene            complement(255..1076)
                     /locus_tag="P5201_13585"
     CDS             complement(255..1076)
                     /locus_tag="P5201_13585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008712042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosome-binding GTPase"
                     /protein_id="HRV99624.1"
                     /translation="MGRTVWYPGHMAKGKRKLSELAGKLDIIIEVRDARAPAQTSSPL
                     VGELSSVCPIAVALSKRDLADEKATAKWLSYFAASGQKAWALDLLKPRMDQVRRDLAI
                     FGPAHREVRLAVVGIPNVGKSMFLNALVGKSSAQVGGIPGITRGVSWYKGKGFLAVDS
                     PGILDPKADDAVQRCLAWLGSSKAEVIGGYENVALDLIAVLRRKNLWGMVEAKWNVAA
                     PAETSDEDVLEALGRRLGCLVSGGAVDLSNAARRFVEAFSTGRLGRVTLELPEES"
     gene            complement(1077..>1406)
                     /gene="lepB"
                     /locus_tag="P5201_13590"
     CDS             complement(1077..>1406)
                     /gene="lepB"
                     /locus_tag="P5201_13590"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008521851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HRV99625.1"
                     /translation="KYPVDPKRDFVKRIIGLPGDMLEVRNGAVLINGQPLQEEYVKNH
                     DSFSLTAGPVFPEVPVKIPANSYFAMGDNRPNSQDSRFWGFVPKQNIRGPVFFRYWPL
                     NRIGMVD"
BASE COUNT          270 a          451 c          420 g          265 t
ORIGIN      
        1 caggatgttc atgcggtcga tccgttcatg agaggcagcc tgcgcgcgcc agagtacgcc
       61 gagagaaagc atcctgccaa agagcgcctc gcgtttccgc ggcgacagct tcttcgagtc
      121 ggcgaggccg agcgcgagca gttcgtcgcg ctgcgtttca gtcaggatga ccgcggcggc
      181 gacgaccggt ccgcagagag gtcctctccc cgcctcgtcg gttccggcga tacgtccgaa
      241 ctccttcagt tccatcagct ctcctccgga agctccagcg tcacgcggcc aagacgcccg
      301 gtcgaaaagg cctcgacaaa acgccgtgcg gcgttcgata gatccaccgc accgccggaa
      361 acaagacagc caagacgtct cccgagcgcc tcgagaacat cttcgtcgga cgtttcagcg
      421 ggtgcggcga cgttccattt ggcctcgacc atcccccaca ggttctttcg gcgaaggacg
      481 gcgataaggt caagagcgac gttctcatac cctccgatga cttccgcctt gctgcttcca
      541 agccatgcaa ggcagcgctg cacggcgtcg tccgctttcg ggtcgaggat tccgggcgaa
      601 tccaccgcga ggaatccctt ccccttgtac catgaaacgc cgcgcgtaat accgggaatg
      661 ccccccacct gcgcggagga ttttcctaca agcgcgttga gaaacatcga tttgccgacg
      721 ttcggaatgc ccacaaccgc cagccgtacc tcgcggtgcg ccggtccgaa aatggcgaga
      781 tcgcgcctga cctggtccat tcttggcttg agcagatcga gcgcccacgc cttctgcccc
      841 gacgcggcaa agtaagagag ccactttgcg gtggcctttt catccgcgag gtcgcgcttc
      901 gaaagggcga cggctatggg gcacacggaa gagagctctc cgacgagcgg agaggatgtc
      961 tgcgccggag cccgcgcgtc gcgcacctcg atgatgatgt ccagtttccc ggcgagttcg
     1021 gaaagctttc gctttccctt ggccatatgg ccgggatacc agacggtacg ccccatctag
     1081 tccaccatcc cgatcctgtt cagaggccag tagcggaaga agactgggcc gcggatgttc
     1141 tgcttcggga caaaccccca gaaacggctg tcctgcgagt tcggcctgtt gtcgcccata
     1201 gcgaagtagg agttcgccgg tatcttgacc gggacctccg gaaagaccgg acccgcggtg
     1261 agcgagaagc tgtcgtgatt cttgacgtac tcttcctgaa gaggctgacc gttgatgagg
     1321 acggcgccgt tgcgcacttc gagcatatcg cccggaagcc cgatgattcg tttgacgaaa
     1381 tcccgcttgg ggtccacggg gtactt
//
LOCUS       DAOTIL010000821         1405 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminobacteriaceae bacterium isolate AMR_MDS_3026
            NODE_110491_length_1405_cov_3.077037, whole genome shotgun
            sequence.
ACCESSION   DAOTIL010000821 DAOTIL010000000
VERSION     DAOTIL010000821.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868050
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminobacteriaceae bacterium (wastewater metagenome)
  ORGANISM  Aminobacteriaceae bacterium
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae.
REFERENCE   1  (bases 1 to 1405)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1405)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:19:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,224
            CDSs (total)                      :: 3,198
            Genes (coding)                    :: 3,184
            CDSs (with protein)               :: 3,184
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1405
                     /organism="Aminobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_110491_length_1405_cov_3.077037"
                     /isolate="AMR_MDS_3026"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:3034609"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..259
                     /locus_tag="P5201_13595"
     CDS             <1..259
                     /locus_tag="P5201_13595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013048887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="BamA/TamA family outer membrane protein"
                     /protein_id="HRV99626.1"
                     /translation="DGGEYEGTKMFLANLELRVPIEKAFGLVLFYDTGNAWLEESMNF
                     SDLHDAWGVGVRVKTPLGNFRLDHATGENETRTHFGFGEMF"
     gene            282..>1405
                     /locus_tag="P5201_13600"
     CDS             282..>1405
                     /locus_tag="P5201_13600"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRV99627.1"
                     /translation="MHPSAHKGVLNGFFKGCVLFFLAAALGFVIPSRACAYAGDIRVE
                     GVPFWLRPSVERSLRSVWNEIAQRSGFSGEDRRALLSVVAERLFVGYSSSVLLSESGV
                     IVTLFPVSPVAWTVEIKTPSLSSPVEEWFAENVAELGGTLERMLALLPLEALSWADIA
                     LNEAIEDVCAPLLPGWNPSLIVRLEGRGIASAPSTADEKRILQISFSPKPPLVLAIVP
                     SISSSTLPVAFRSDLREKILRTLAPVVGLPIEWAAKNKLRIEALAAEALFDTNTVSNA
                     RATVDVSFSPEQLSPVQAEVESPRYTIRAWIAAYAGAEGKYPEIGLHLGRNALPLSGW
                     DVELYGEWILAANDFSLESRWGARWSPWKHILIGAEAAFPG"
BASE COUNT          265 a          384 c          427 g          329 t
ORIGIN      
        1 tgacggcggg gagtacgagg gaacgaagat gttcctggcc aaccttgaac tgcgtgttcc
       61 tatcgagaag gcgtttggcc ttgttctctt ctacgatacg ggcaacgcat ggcttgaaga
      121 gagcatgaac ttttccgatc ttcatgatgc gtggggagtc ggcgtcaggg taaagactcc
      181 tctggggaac ttccggctcg accatgccac gggagagaat gaaacacgaa cgcatttcgg
      241 ttttggagaa atgttctaga gcgcccgaag gaggcgccga tgtgcatcct tctgcgcaca
      301 agggagtatt gaacggattc ttcaagggct gtgtgctgtt tttccttgcc gcagcccttg
      361 gcttcgttat cccctcccga gcgtgcgcat acgccgggga tatccgggtc gaaggcgtcc
      421 cgttctggct tcgtccaagc gtcgagagga gtctgcgttc tgtatggaac gagattgcgc
      481 agcgctccgg attttccgga gaagatcgcc gtgcgctcct ctccgtcgtt gcggagaggc
      541 ttttcgttgg ctactcctcc tcggttttgc tctccgagtc cggtgtgatt gtcacccttt
      601 tcccggtttc gcccgtcgcg tggacggtgg agataaaaac gccgtcgctt tcctccccgg
      661 ttgaggagtg gtttgctgaa aatgtcgcgg agctcggcgg gacgctggaa cggatgcttg
      721 cgttgctccc gctcgaggcg ctttcttggg ccgatatagc attgaatgaa gcgattgaag
      781 acgtatgcgc gcctctcctt cccggctgga acccgtccct catcgtgcgc cttgaaggga
      841 ggggaattgc aagcgcaccg agtacagcgg acgaaaaacg tattctgcag atctcttttt
      901 ctcccaaacc tcccttggtg ctggctatcg ttccgtccat ctcttcctcc acgctgcccg
      961 tagcctttcg ctccgacctg agggagaaga tattgaggac gcttgctccg gttgtcggtc
     1021 tgccaataga gtgggcggca aaaaacaagc tccgtataga agctcttgcc gcagaggcac
     1081 ttttcgatac caatacggtg tcgaacgccc gggcgaccgt tgacgtttct ttctcccccg
     1141 agcaactctc ccccgtgcaa gcagaagtgg agagcccgag atatactatc cgggcgtgga
     1201 tagccgcgta tgccggagcg gaggggaagt accctgagat cggccttcac ctgggaagaa
     1261 acgcgctgcc gctctccggg tgggatgtag agctctacgg cgaatggata ctcgcagcca
     1321 acgatttttc gttggaaagc agatggggag cgcgctggag tccgtggaag cacatcctca
     1381 tcggagcgga agcggccttt ccggg
//
LOCUS       DAOTIL010000822         1404 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminobacteriaceae bacterium isolate AMR_MDS_3026
            NODE_110577_length_1404_cov_4.126019, whole genome shotgun
            sequence.
ACCESSION   DAOTIL010000822 DAOTIL010000000
VERSION     DAOTIL010000822.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868050
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminobacteriaceae bacterium (wastewater metagenome)
  ORGANISM  Aminobacteriaceae bacterium
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae.
REFERENCE   1  (bases 1 to 1404)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1404)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:19:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,224
            CDSs (total)                      :: 3,198
            Genes (coding)                    :: 3,184
            CDSs (with protein)               :: 3,184
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1404
                     /organism="Aminobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_110577_length_1404_cov_4.126019"
                     /isolate="AMR_MDS_3026"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:3034609"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            89..>1404
                     /gene="feoB"
                     /locus_tag="P5201_13605"
     CDS             89..>1404
                     /gene="feoB"
                     /locus_tag="P5201_13605"
                     /inference="COORDINATES: protein motif:HMM:TIGR00437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015093 - ferrous iron transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006826 - iron ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferrous iron transport protein B"
                     /protein_id="HRV99628.1"
                     /translation="MTVERKEGRFRSSGDEVTVVDLPGIYSLGAASLDEEIAADYLLR
                     NRPDLAVVVADASNLERSLYLAVQMLEIGTPLVLALNMMDMAAERGIMIDVERLSALL
                     GIPVVPTVARKKEGIEELKERITRSLRENSAPSLSFALPYGERLNPLFEALETFLGER
                     QELIPGLSARSAAVKFVEGDVAVLAAAESSGVRNSMDELLAEKGRKVEEELGYDLQTA
                     VIERRWGFVSGVTAESVQRDLSLKARLTLSDRIDRVVTSRSLGLPLFFLVTWAIFRMT
                     YALGDPLVELLEGVFESFGEWTGEFFAGAGFSEIVSSFVQDGLIGGVGSVVVFFPHIF
                     ILFAFIALLEDSGYMARGAFVMDRVMHLMGLHGKSFIPMLMGFGCNVPSMMATRILEQ
                     PRDRMITLLVLPFMSCSARLPVFVLFSGIFFGSYAGTAVFSLYVLG"
BASE COUNT          243 a          370 c          450 g          341 t
ORIGIN      
        1 cagcaaggtc aaggagaagc ggtagcgctg gcggagtctc ttcaatgcgc tcactggctc
       61 caatcagcac gtggggaact ggcccggcgt gactgtcgaa cgaaaagagg ggcgattccg
      121 ttcctcggga gacgaggtga ctgtggtcga tcttcccggc atttacagcc tgggggcggc
      181 gtcgctcgac gaggagatcg cggcggacta tctgttgcgg aaccggcccg acctggctgt
      241 tgtcgtggcg gatgcgtcca acctggaacg cagcctctat cttgccgtgc agatgcttga
      301 gatcggcact ccgctcgtcc ttgcgctgaa catgatggac atggcggcgg agaggggaat
      361 tatgatcgac gtggaacgcc tttcggcgct tcttggtatt cccgtcgtgc cgaccgtcgc
      421 acggaagaag gaaggaatag aagaactcaa agagagaatt actcgttctc ttcgggagaa
      481 ctcggcgccc tctctttctt tcgctctgcc gtacggagaa cgcctgaatc ccttgttcga
      541 agcgcttgaa acgtttctgg gggagcgcca ggaactcatt ccggggcttt ccgcccgttc
      601 cgcggcggtg aagttcgtcg agggcgacgt tgcggttctt gcggccgcgg agagcagcgg
      661 cgttcgcaac agcatggacg aactccttgc cgagaagggg cgtaaggtgg aggaggagtt
      721 gggatacgat ctccaaactg ccgtgatcga aagaaggtgg ggctttgttt cgggcgtgac
      781 ggcggaatcg gtacaacgcg acctctcgct caaagcgcgc ctgacccttt cggatcgcat
      841 cgaccgcgtg gtgacttcgc gatctctagg gttgccgctc ttctttctcg tcacatgggc
      901 aatattccgc atgacctacg ccttaggcga cccgttggtg gagctcctgg aaggagtgtt
      961 cgaatccttc ggggaatgga cgggcgagtt tttcgccggc gccgggtttt cggagatcgt
     1021 ctcatccttc gtacaggacg ggctcattgg gggcgtcggc tcggtcgtcg tcttcttccc
     1081 tcatatcttc atccttttcg cgttcatcgc attgctggag gattcggggt atatggcgcg
     1141 gggtgcgttt gtgatggaca gggttatgca cctgatgggg ctgcacggca agagtttcat
     1201 ccccatgctc atgggttttg ggtgcaacgt gccttcgatg atggcgacgc gtattcttga
     1261 acagcccagg gacagaatga tcacgctgct tgtgctgcct tttatgagct gctcggctcg
     1321 tcttcctgtc tttgtgctct tctcgggaat tttcttcgga tcgtatgcgg gaacggccgt
     1381 cttctcactg tatgtcctcg gaat
//
LOCUS       DAOTIL010000823         1401 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminobacteriaceae bacterium isolate AMR_MDS_3026
            NODE_110961_length_1401_cov_2.271174, whole genome shotgun
            sequence.
ACCESSION   DAOTIL010000823 DAOTIL010000000
VERSION     DAOTIL010000823.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868050
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminobacteriaceae bacterium (wastewater metagenome)
  ORGANISM  Aminobacteriaceae bacterium
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae.
REFERENCE   1  (bases 1 to 1401)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1401)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:19:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,224
            CDSs (total)                      :: 3,198
            Genes (coding)                    :: 3,184
            CDSs (with protein)               :: 3,184
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1401
                     /organism="Aminobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_110961_length_1401_cov_2.271174"
                     /isolate="AMR_MDS_3026"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:3034609"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1003)
                     /locus_tag="P5201_13610"
     CDS             complement(<1..1003)
                     /locus_tag="P5201_13610"
                     /inference="COORDINATES: protein motif:HMM:NF014442.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008170 - N-methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006306 - DNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-6 DNA methylase"
                     /protein_id="HRV99629.1"
                     /translation="MSAGSALIRSIEVPGNRMAFRDKLILSLQIAAWFKLSRTGKLSR
                     DLAFDSGHRRMTWRTLFETFERIRNEAASEEIARSFRPCGGTGASMDAVQLSNVFQLA
                     SNLEDGGFFGREFVDEVLEMLAREKEFAESVLPAEVVRLMLEVADIRPGERIYTPFDN
                     CLQLSIAVEEQSGGNAPVFAEFPEASALPWLLRLFYGSASAIKEGDPVLAPSYFSGER
                     TLDRFDTALAVLPFSKKYARFDAGRDPLDRFPEATASAGVLALRHILAQTKKRAVAAV
                     ANSLLFSSGAERALREHLVDTGKVEAVIGLPSALLAGSSVAFSLLVLKLDAPSETIRF
                     "
     gene            complement(1216..>1401)
                     /locus_tag="P5201_13615"
     CDS             complement(1216..>1401)
                     /locus_tag="P5201_13615"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRV99630.1"
                     /translation="AEKLRARGVVLDAPALSERFYESGLSENSGWGYMRDGDDEPVHY
                     IDRTKKYTRPPRYDSKS"
BASE COUNT          271 a          425 c          410 g          295 t
ORIGIN      
        1 tgaagcgtat tgtttccgaa ggggcgtcca gcttcaatac cagaagcgag aatgcaaccg
       61 acgatccggc gagcagggcg gagggcaggc cgataacagc ttctaccttc cctgtatcca
      121 caagatgctc ccgcagcgcg cgctccgctc ccgaactgaa gagcaggctg ttggcgacag
      181 ccgcgacggc ccgctttttt gtctgggcaa gaatgtgtct gagggcgagc acgcctgccg
      241 aggcagtcgc ttccgggaag cgatccaatg ggtctctccc ggcgtcgaaa cgggcgtatt
      301 ttttggagaa cggcagaacg gcgagagcgg tgtcgaaccg gtccagcgtc ctttctccgg
      361 aaaaatagga gggcgcgagg acggggtccc cctccttgat cgcggacgcg cttccgtaga
      421 agaggcgcag cagccagggc agcgccgacg cttccggaaa ctcggcgaag accggagcgt
      481 tccccccgct ctgttcctcg accgcaatgc tcagttgcag gcagttgtca aaaggcgtgt
      541 agatgcgttc tcctgggcgt atatccgcaa cttcgagcat cagccggacc acttccgccg
      601 gaaggacgga ctccgcaaac tccttctcgc gggcgagcat ttcgagtact tcatcgacga
      661 actccctgcc gaaaaacccg ccgtcttcaa ggttggacgc aagctggaag acgttcgaaa
      721 gctggaccgc atccatgctc gcacctgttc cgccgcacgg acggaacgaa cgggcgatct
      781 cctccgacgc ggcttcgttc cggattcttt cgaaagtctc gaacagagtc cgccaggtca
      841 tcctgcgatg cccggaatcg aaggcaagat ctcgggacag cttccccgtt ctggacagct
      901 tgaaccacgc ggcgatctgc aaggaaagaa tcagcttgtc acgaaacgcc attctgtttc
      961 ccggaacctc tattgatcgg ataagcgcgc ttccggcact catcggctat tcctccctcg
     1021 ccgccgcccc gcgcggtacc ctgcggagtc ggcgtcctct cgttgcgttc atttctcttc
     1081 aagcgatact atatcatgga aaggcgtgct tgtaaatagc acgtcgtgct ttttattttc
     1141 cggctctgct tttacttgga ggattcttga aggagggatc cgaagggtcg cgtttgttct
     1201 tccgggaagg tctgcttacg acttggaatc gtaccgggga gggcgtgtgt actttttcgt
     1261 gcggtcgatg tagtggacgg gctcgtcatc gccgtccctc atgtaccccc accccgaatt
     1321 ctcggagagt ccggattcgt aaaaccgttc ggagagcgcc ggggcgtcca gcacgacgcc
     1381 ccgcgcccgc agtttttctg c
//
LOCUS       DAOTIL010000824         1400 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminobacteriaceae bacterium isolate AMR_MDS_3026
            NODE_111039_length_1400_cov_3.211896, whole genome shotgun
            sequence.
ACCESSION   DAOTIL010000824 DAOTIL010000000
VERSION     DAOTIL010000824.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868050
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminobacteriaceae bacterium (wastewater metagenome)
  ORGANISM  Aminobacteriaceae bacterium
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae.
REFERENCE   1  (bases 1 to 1400)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1400)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:19:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,224
            CDSs (total)                      :: 3,198
            Genes (coding)                    :: 3,184
            CDSs (with protein)               :: 3,184
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1400
                     /organism="Aminobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_111039_length_1400_cov_3.211896"
                     /isolate="AMR_MDS_3026"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:3034609"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..664)
                     /locus_tag="P5201_13620"
     CDS             complement(<1..664)
                     /locus_tag="P5201_13620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009165970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal-5-phosphate-dependent protein subunit
                     beta"
                     /protein_id="HRV99631.1"
                     /translation="MSYSIDLTVHKEGLKNAVEVAKKRNIVIPTFKQMKDPEHHTPAA
                     IKEKLKKTGLWDVDSANLFRITWKNQPTKTGGLFGKVNYIELPKELTGVDARIIAIVG
                     KWFPTGAHKVGASFGCLAPRLVTGQFDPIHQKAVWPSTGNYCRGGAYNAQLLGCESIA
                     ILPQGMSRERFDWLETVAGEIIATPGTESNVKEIYDKVWELRRTRDNIVVFNQFDEMG
                     NHL"
     gene            complement(683..>1400)
                     /locus_tag="P5201_13625"
     CDS             complement(683..>1400)
                     /locus_tag="P5201_13625"
                     /EC_number="2.1.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014163863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004372 - glycine hydroxymethyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0019264 - glycine biosynthetic process from
                     serine [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="serine hydroxymethyltransferase"
                     /protein_id="HRV99632.1"
                     /translation="EIDAERFRGIADKVGALLFFDIAHIAGLIAAGCHKDPVPFCHFL
                     TSTTHKTLRGPRGGMIMTTNEYAKAIDKNIFPGMQGGPLMHVIAAKAVAFREALKPSF
                     KEYQKKVVANAKALAEEFLSLDYHLVSGGTDNHLILVNLMTKGVTGKAAEAALDKAGM
                     TVNKNAVPFDTQSPFITSGIRVGTPAATTRGFGVDDMKQIARWIDRAVRNVENESELA
                     TIKAEVLESCNKHPLYPGLE"
BASE COUNT          255 a          416 c          409 g          320 t
ORIGIN      
        1 agaggtggtt ccccatctcg tcgaactggt tgaagacgac gatgttatcc cgcgtgcggc
       61 ggagctccca gaccttgtcg taaatctcct tgacgttgct ctccgtcccc ggagtagcga
      121 tgatctcgcc cgcaacggtc tcaagccagt cgaaacgctc ccggctcatc ccctggggaa
      181 gtatggcgat ggattcgcac cccagcagtt gcgcgttgta ggcgccgccg cggcagtaat
      241 tccccgtgga gggccagacg gccttctgat gaatcgggtc gaactgcccg gtgacaagac
      301 gcggtgcgag gcagccaaag cttgccccga ccttgtgcgc cccagtcggg aaccacttgc
      361 cgacaatggc gatgatacga gcatctacgc cggtaagctc cttaggaagc tcgatgtagt
      421 tcacttttcc gaaaagaccg cctgttttcg tcggctgatt cttccaagtg atgcgaaaaa
      481 ggttcgcaga gtctacgtcc cagagtccgg tcttcttcag tttttccttg attgcggccg
      541 gggtgtgatg ctccggatcc ttcatctgct tgaatgtagg aatgacgatg ttgcgcttct
      601 tcgccacctc tacggcgttc ttcaggcctt ctttatgaac ggtcagatcg atcgaatacg
      661 acatatcgct cctccttact ccttattcca accccgggta cagggggtgc ttgttgcagc
      721 tctccagaac ctcggccttg atcgtcgcaa gctcggattc attctcgacg ttccgcacag
      781 cgcggtcgat ccagcgggcg atctgcttca tgtcgtcgac gccgaagccg cgagtcgtgg
      841 ccgcgggcgt gccgacccgg atgccgctgg tgatgaaggg gctctgggta tcaaaaggca
      901 cagcattctt gttgaccgtc attccagcct tgtccagcgc tgcttccgcg gcctttccgg
      961 tgacgccctt ggtcatcagg ttcaccagaa tcaggtggtt atccgtaccg ccggagacca
     1021 gatgataatc gagggagagg aactcttccg cgagcgcttt cgcgttcgcc acaaccttct
     1081 tctgatactc cttgaaggac ggcttgagcg cttcgcggaa cgccacggcc ttggcggcga
     1141 tcacgtgcat cagaggaccg ccctgcatgc cggggaagat gttcttgtcg atggctttcg
     1201 catactcgtt cgtggtcatg atcatgccgc cgcgaggtcc gcggagcgtc ttgtgcgtcg
     1261 tcgaggtaag gaagtggcag aagggaaccg gatccttgtg gcatccggcg gcgatcaatc
     1321 cggcgatatg ggcgatgtcg aagaaaagca gcgcgccgac cttgtccgcg atacccctga
     1381 accgctcggc gtcgatctcg
//
LOCUS       DAOTIL010000825         1400 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminobacteriaceae bacterium isolate AMR_MDS_3026
            NODE_111049_length_1400_cov_2.916729, whole genome shotgun
            sequence.
ACCESSION   DAOTIL010000825 DAOTIL010000000
VERSION     DAOTIL010000825.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868050
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminobacteriaceae bacterium (wastewater metagenome)
  ORGANISM  Aminobacteriaceae bacterium
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae.
REFERENCE   1  (bases 1 to 1400)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1400)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:19:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,224
            CDSs (total)                      :: 3,198
            Genes (coding)                    :: 3,184
            CDSs (with protein)               :: 3,184
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1400
                     /organism="Aminobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_111049_length_1400_cov_2.916729"
                     /isolate="AMR_MDS_3026"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:3034609"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1190)
                     /locus_tag="P5201_13630"
     CDS             complement(<1..1190)
                     /locus_tag="P5201_13630"
                     /inference="COORDINATES: protein motif:HMM:NF015859.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHASE domain-containing protein"
                     /protein_id="HRV99633.1"
                     /translation="MKKTFFRSSHKRAASLADILVVVLLLAAGFFLGEWRVSKAELGM
                     RHNLVRRARLFAESVNPRAVKRLSGSESDRVSPDYRRFKEQSISLLSAFPDCRFISLL
                     GRRADGSIFIYFDSEPEGSPDESPPGERYVPPDPVLLEAFDKGKSVVAGPFMDIWGSL
                     VAAWVPVADPDTGKPAFVAALDIDGGKWRSDARRAAYVPECAAVLLAALFIVWRILTL
                     RRALRRRTTYRWYHRYADALFVAATGLVLTFSILLVSSEVERSTREDTFHMLADSESG
                     KLHNSLKSIRDIGVEGAAAFFSYSDFVDFEEFKGYARHLEELPEVRGATWLPLVPHEE
                     REAFERLGRSLVTEDFVIRDYGEGGRRGPAPGRPFYYPVFYRIPFEGSERILGADFAQ
                     VPSR"
BASE COUNT          297 a          441 c          427 g          235 t
ORIGIN      
        1 cttctggagg gaacctgagc aaagtcagcc cccagtatgc gctcgctccc ctcaaagggt
       61 atgcggtaga acaccggata ataaaaggga cgcccaggag cggggcccct ccttcccccc
      121 tccccgtaat cgcggatgac gaaatcctct gtaacgagcg agcgtccgag acgttcgaac
      181 gcctcgcgct cttcatgcgg aacgagcgga agccacgtcg cgccccgcac ctcgggcaac
      241 tcctccagat gacgggcgta ccccttgaat tcctcgaagt cgacgaaatc gctgtacgag
      301 aagaacgccg ccgccccctc gacgccgata tccctgatgc tcttcaggct gttgtgcagc
      361 ttccccgact ccgagtcggc aagcatgtga aaggtatcct cacgcgtcga gcgctcaacc
      421 tccgatgaga cgagaagtat cgaaaatgtc agcacgagcc cggtcgcggc gacgaagaga
      481 gcgtcggcgt agcggtgata ccaacggtag gtagttctcc tcctcagggc gcggcgcagc
      541 gtcagtatcc gccatacgat aaagagagcg gccagaagga ccgccgcgca ttccggaacg
      601 tatgcggcgc gtcgggcgtc gctccgccac ttcccgccgt cgatatcgag cgcggcgacg
      661 aacgcgggct tcccggtatc cgggtcggcg acgggaaccc acgccgcgac gaggcttccc
      721 caaatatcca taaagggacc ggcgacaacg ctcttcccct tgtcgaaggc ctccagcagt
      781 acgggatccg gaggaacata cctctctccg gggggcgact cgtccggcga gccttcgggc
      841 tcggaatcga agtaaatgaa gatcgacccg tcggcacggc ggccaaggag cgagatgaaa
      901 cggcagtcgg gaaaggcgga gaggagcgaa atgctctgtt ctttgaagcg gcggtagtcc
      961 ggagaaacgc ggtccgattc ggaacctgag aggcgcttga ccgcgcgagg attgacgctt
     1021 tcggcgaaaa ggcgcgctcg gcggaccagg ttatggcgca tccccagctc tgccttcgac
     1081 acgcgccact cgccgaggaa aaatccagcc gccagcaaga gcacgacgac aagaatatcg
     1141 gcgagactcg cggcgcgttt atgcgatgaa cggaaaaagg tcttcttcat aggcgcctcc
     1201 gggattacgg ctcttcatgt cccgcggcaa cggggccgta ggccccgcta gagtttcacg
     1261 ggattcctct aagtatactc tattctccgc ccgaccgttc gctacttttt aagacgcagc
     1321 cgctcagtct ccttcgagag ggcggaggcg cgttatggcg cggatccgtt ccctgagagg
     1381 agggcgatcg cgttccaccg
//
LOCUS       DAOTIL010000826         1399 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminobacteriaceae bacterium isolate AMR_MDS_3026
            NODE_111132_length_1399_cov_3.968006, whole genome shotgun
            sequence.
ACCESSION   DAOTIL010000826 DAOTIL010000000
VERSION     DAOTIL010000826.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868050
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminobacteriaceae bacterium (wastewater metagenome)
  ORGANISM  Aminobacteriaceae bacterium
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae.
REFERENCE   1  (bases 1 to 1399)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1399)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:19:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,224
            CDSs (total)                      :: 3,198
            Genes (coding)                    :: 3,184
            CDSs (with protein)               :: 3,184
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1399
                     /organism="Aminobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_111132_length_1399_cov_3.968006"
                     /isolate="AMR_MDS_3026"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:3034609"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1399)
                     /locus_tag="P5201_13635"
     CDS             complement(<1..>1399)
                     /locus_tag="P5201_13635"
                     /inference="COORDINATES: protein motif:HMM:NF012321.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="vWA domain-containing protein"
                     /protein_id="HRV99634.1"
                     /translation="RIFQGPSSFLSAYRLSDIVWEKAGHPKAAYGAYGSYDAQKPLGF
                     RMAMFDGFKEYASGTAICDFLAPEHRMYAGYALASLSSYYIHDLREEYAIERGGKKYN
                     ALAYPSILNDVSLAAYAGDLRALNHSTPRLYADAPSTVQKAKWGISAWEYLTKRRAFP
                     AQLAPVPPKNKRDASLNESEYRFLIQPGKHNAEAQSRLRIVWVSSPVFQIVIDRSGSM
                     EGERIVKAKNAAKDFVRSLQPGVRVGVIVFDDAVNVVSPIVAIGSGDAEKKKLYSAID
                     GISLGGATAIYDACAVASKEMTADRNSYFEGRYVVLFTDGEDNRSKIGVAPLIQALRK
                     ENVSVLVMGLEDEVREKTLREIASGTKGYYMMTADPLDTQEMLYGYLNSAFESQISTF
                     DATLPAGGSTRTAFAVEEGTELLRVSLVSDGEISPKTLVFSATPPKGKASAALHDKET
                     GKLEILQKKPAAGKWT"
BASE COUNT          264 a          399 c          425 g          311 t
ORIGIN      
        1 ggtccatttc ccggccgcgg gtttcttctg aaggatctcc agcttcccgg tttccttatc
       61 gtgaagcgcg gcggaagctt tgcccttcgg gggagtcgcg gagaatacga gcgtcttggg
      121 cgagatctct ccgtcggaaa caagagagac ccgcagcagt tccgtgcctt cctccacggc
      181 gaacgccgtc cgcgtcgagc cgcctgcagg aagcgttgcg tcgaacgtgg atatctggct
      241 ttcgaaggcg ctgttgagat agccgtagag catttcctgg gtgtccagcg gatcagccgt
      301 catcatgtag tatcctttgg ttcctgatgc gatctcgcgg agcgtcttct cgcgcacttc
      361 gtcctcgagt cccatgacga gcactgaaac gttctccttc cgcagcgctt gaatcagggg
      421 agcgacgccg attttagagc ggttgtcctc tccgtcggtg aagaggacga cgtagcgtcc
      481 ctcgaaatac gagtttctgt ccgcagtcat ctccttcgac gcgacggcgc aggcgtcgta
      541 gatcgcggtt gctcccccga gcgaaatgcc gtcgatagcg gagtagagct tctttttttc
      601 ggcatccccc gagccgattg cgacgatggg tgagacgacg ttcacagcgt cgtcgaagac
      661 gatcacgccg acccgcactc caggctgaag cgagcggacg aagtccttgg cggcgttttt
      721 cgccttgacg atccgctctc cttccatgct gccggaacgg tcgatgacaa tctgaaaaac
      781 gggggaggaa acccagacga tacgcaggcg gctttgcgcc tccgcattgt gttttcccgg
      841 ctggatgaga aaacggtatt cgctctcgtt cagagaagcg tccctcttgt tttttggcgg
      901 cacgggggcc aactgcgccg gaaacgcacg ccgtttcgtc agatattccc acgctgaaat
      961 tccccacttc gccttttgga cggtcgaagg cgcatccgca taaaggcgcg gggtggaatg
     1021 gttcaatgcg cgcaagtctc ccgcgtacgc ggcaagcgag acgtcgttga gtatgctggg
     1081 ataggcgagg gcgttgtact tcttcccgcc ccgttcgatc gcgtattctt ccctaaggtc
     1141 atggatgtaa taacttgaga ggcttgcaag cgcgtatccc gcgtacatcc ggtgttcggg
     1201 agcaagaaaa tcgcagatgg ccgttcccga agcatactct ttgaagccgt cgaacatggc
     1261 catgcggaat ccgaggggtt tctgcgcgtc gtaggagccg tacgcgccgt aggccgcttt
     1321 gggatggccg gccttctccc agacgatgtc ggagagacgg taggccgaga gaaagcttga
     1381 aggcccctga aaaatgcgt
//
LOCUS       DAOTIL010000827         1398 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminobacteriaceae bacterium isolate AMR_MDS_3026
            NODE_111238_length_1398_cov_7.096798, whole genome shotgun
            sequence.
ACCESSION   DAOTIL010000827 DAOTIL010000000
VERSION     DAOTIL010000827.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868050
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminobacteriaceae bacterium (wastewater metagenome)
  ORGANISM  Aminobacteriaceae bacterium
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae.
REFERENCE   1  (bases 1 to 1398)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1398)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:19:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,224
            CDSs (total)                      :: 3,198
            Genes (coding)                    :: 3,184
            CDSs (with protein)               :: 3,184
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1398
                     /organism="Aminobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_111238_length_1398_cov_7.096798"
                     /isolate="AMR_MDS_3026"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:3034609"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            98..1351
                     /locus_tag="P5201_13640"
     CDS             98..1351
                     /locus_tag="P5201_13640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS256 family transposase"
                     /protein_id="HRV99635.1"
                     /translation="MIKNTIKTRIEATPETMGALEIIVREGACKMLQAALEAEIEEHI
                     SRFKDLVDDDGKRLVVRNGTMPERTVLTGAGPLPITRPRVDDRKLESLGKERFTSRIL
                     PPFMRRAPSIDTLVPVLYLKGISTDDFPTALEAILGPQAKGLSASTVVRLKEIWTEEY
                     GDWSKRDLSGKRYVYIWADGVYCNARLEDERSCLLVVMGADSFGNKELLAVHDGYRES
                     TQSWKEILLDLRSRGLAKAPALAVCDGAMGFQAACAEVWPETRIQRCWFHKSGNVLDK
                     LPKALQSKAKDMLHDQYLATTREDALKAFALFVESFGAKYPKAVECLVKDKDDLFAFY
                     DFPAMHWIHLRTTNPIESTFATVRLRHRRTKGNGTRKATLAMVYKLCREAEQGWRKLD
                     GYKFIPLVEAGVKFVNGEQVDGTAA"
BASE COUNT          331 a          363 c          420 g          284 t
ORIGIN      
        1 tgagatataa tcgattcttg tggacgggat agaaaaagga cgtttcttca cggtaaggtt
       61 ttgtcgccaa acaagactcg ccgggaggag aacgtccgtg atcaagaata ccatcaagac
      121 tcgaatcgaa gcaacgcccg aaaccatggg agcgttggag atcatcgtac gcgagggggc
      181 atgtaagatg ttgcaggcag ccctcgaggc ggagatcgag gaacatattt cgcggttcaa
      241 agacctcgtc gacgatgacg ggaaacgtct tgtcgtacga aacggaacca tgcccgagcg
      301 cacggtgctg accggagccg gcccgcttcc gatcacccgg ccccgcgtgg atgaccggaa
      361 gctcgaaagt cttgggaaag agcgctttac gagcaggata cttccgcctt tcatgcgaag
      421 ggcaccgagt attgatacgc tcgttcccgt gctctacctc aaggggattt cgacagacga
      481 cttcccgacg gcacttgaag cgattttggg accgcaggcg aaaggtcttt cggcgagtac
      541 cgtcgttcgg ctcaaggaaa tctggacgga agaatacggc gattggtcga aacgcgatct
      601 gtcgggaaag cggtatgtgt atatctgggc agatggcgtg tattgcaatg cacgcctcga
      661 agatgaacgc tcctgcctgc tcgtcgtcat gggtgcggat tcctttggaa ataaagagtt
      721 gcttgccgtt catgacggat atcgcgaaag cacccaatcg tggaaggaaa tcctcctgga
      781 tctgcgctcc cgcggacttg ctaaagcgcc agcgctcgcc gtatgcgatg gtgcgatggg
      841 cttccaggcc gcctgcgccg aggtctggcc cgagacgcgc atccagcgct gctggttcca
      901 caaatcgggg aacgtcctcg acaagctccc caaggcactg caatcgaagg cgaaggatat
      961 gctccacgat cagtacttgg cgacgacgcg cgaggatgcg ttgaaggcgt tcgcgctctt
     1021 cgtggaatcc ttcggcgcaa aatatccgaa ggcggtggaa tgtctcgtca aggacaagga
     1081 cgatctcttc gccttctacg atttcccggc catgcactgg atacatttac gcaccacgaa
     1141 tccgatcgaa tcgaccttcg cgacggttcg actgcgtcac cggcgaacga agggtaacgg
     1201 gacgcgcaag gcgactttgg caatggtcta caagctatgc agagaggcag agcaaggctg
     1261 gcgcaagttg gatggataca agttcatccc cctcgttgag gcgggagtaa agtttgtgaa
     1321 tggcgagcaa gttgatggta cggcagcctg accggtggag cgtccggatt ttcaatccac
     1381 aactattgac aatatctc
//
LOCUS       DAOTIL010000828         1397 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminobacteriaceae bacterium isolate AMR_MDS_3026
            NODE_111366_length_1397_cov_3.947839, whole genome shotgun
            sequence.
ACCESSION   DAOTIL010000828 DAOTIL010000000
VERSION     DAOTIL010000828.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868050
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminobacteriaceae bacterium (wastewater metagenome)
  ORGANISM  Aminobacteriaceae bacterium
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae.
REFERENCE   1  (bases 1 to 1397)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1397)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:19:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,224
            CDSs (total)                      :: 3,198
            Genes (coding)                    :: 3,184
            CDSs (with protein)               :: 3,184
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1397
                     /organism="Aminobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_111366_length_1397_cov_3.947839"
                     /isolate="AMR_MDS_3026"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:3034609"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..285)
                     /locus_tag="P5201_13645"
     CDS             complement(<1..285)
                     /locus_tag="P5201_13645"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRV99636.1"
                     /translation="MRSAVMYVSEAGKSEVALFKADENLRLHVLGVHTLDIAPYRDTY
                     YPPDQFDIAFADWEGDGRKKIFCVTGYAGDGYGRNVRLLTVTIQEGRATFT"
     assembly_gap    536..545
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<546..992)
                     /locus_tag="P5201_13650"
     CDS             complement(<546..992)
                     /locus_tag="P5201_13650"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRV99637.1"
                     /translation="MFRRKMKGSLLVALFLLAALLPQAPRLEAAPPGTTDPFARAPFL
                     LMDEEAVVLTQESGNIYSDVFLPNVVQREDGSRTWSERLDDAKATWSPRPPLLDGRVG
                     DAAVLLRNSRNEAFLAQVWNAISSTGLTMSLAPVDQNGYKAEIMRIN"
     gene            complement(1008..>1397)
                     /locus_tag="P5201_13655"
     CDS             complement(1008..>1397)
                     /locus_tag="P5201_13655"
                     /inference="COORDINATES: protein motif:HMM:NF013088.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HRV99638.1"
                     /translation="AAAGQVTASSIILVPLALVLEHPWTLPAPAPEVWGAILGLALLS
                     TAFAYVIYFRILASAGANNVLLVTFLIPVSAILLGTTVLGERLEPKHFAGMALIGVAL
                     AAIDGRLFGMWRRFFRTSPGDNPGDCE"
BASE COUNT          269 a          431 c          427 g          260 t
ORIGIN      
        1 ggtgaaggtc gctcttcctt cctggatggt cacggtgagc aggcggacgt ttcttccgta
       61 cccgtctccg gcgtaccctg taacgcagaa gatcttcttc cttccatcgc cttcccagtc
      121 ggcgaaggcg atgtcgaact ggtccggggg gtagtatgtg tcgcggtagg gcgcgatgtc
      181 gagcgtatgg acgccgagga catgaagcct cagattctca tccgccttga aaagcgccac
      241 ctcggacttt cccgcctccg atacgtacat cacggcggat ctcatccctg ccgttcccgt
      301 cggcgtcgcc ggtcgcgatg cggaatccgg agcgccagtg tatgcgaggt acttcgcctg
      361 cgttcgaaaa ctcgcttcca acgggattgt cgaatccggc gacggaggcc gtgaaaaata
      421 tcggtccgtt actatcgccg gtactgacca agacaagttc cgtggcccga tccccgttaa
      481 gatcccccgc ggcggcgacg aaataattga ttgaaaagaa accccatcta ctattnnnnn
      541 nnnnnattta ttcgcataat ctccgccttg tagccattct ggtccacagg cgccaacgac
      601 atcgtcaacc ccgtcgaaga gattgcattc cagacctgcg ccagaaacgc ctcgttccgg
      661 ctgttccgta gcagcacggc cgcgtcgccg acccgtccgt ccagaagcgg cggtctcggc
      721 gaccaggtcg ccttggcatc gtccagtcgt tcggaccagg tgcgggagcc gtcctcgcgc
      781 tgcaccacgt tcggcaggaa gacgtcgctg tagatgttgc cgctctcctg cgtcaggacg
      841 accgcctcct cgtccatcag caggaagggc gcgcgggcga acgggtccgt cgttcccggc
      901 ggagctgcct ccaagcgggg ggcctgcgga agcagcgctg cgagcagaaa caacgcaaca
      961 agcagagaac ctttcatttt tcgtcggaac atgtcggtac ctccttttca ctcgcaatct
     1021 cccgggttgt ctcccgggga agtacggaaa aagcgccgcc acatgccgaa gagcctgccg
     1081 tcgatcgcgg cgagggcgac gccgatgagc gccattccag cgaagtgctt cggctccaga
     1141 cgttctccga ggacggtggt tccgaggagt atggcgctga ccgggatgag aaaggtcaca
     1201 aggagcacgt tgttggctcc cgccgaggcc agtatgcgga agtagatgac gtaggcgaac
     1261 gcggtggaga gcagcgcaag cccgagaatc gcgccccaca cctcgggcgc cggcgctgga
     1321 agcgtccacg gatgttcaag gacgagggcg agcggtacga ggatgatgct ggacgccgtt
     1381 acctgcccgg cggcagc
//
LOCUS       DAOTIL010000829         1397 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminobacteriaceae bacterium isolate AMR_MDS_3026
            NODE_111404_length_1397_cov_2.636364, whole genome shotgun
            sequence.
ACCESSION   DAOTIL010000829 DAOTIL010000000
VERSION     DAOTIL010000829.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868050
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminobacteriaceae bacterium (wastewater metagenome)
  ORGANISM  Aminobacteriaceae bacterium
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae.
REFERENCE   1  (bases 1 to 1397)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1397)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:19:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,224
            CDSs (total)                      :: 3,198
            Genes (coding)                    :: 3,184
            CDSs (with protein)               :: 3,184
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1397
                     /organism="Aminobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_111404_length_1397_cov_2.636364"
                     /isolate="AMR_MDS_3026"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:3034609"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1303)
                     /locus_tag="P5201_13660"
     CDS             complement(<1..1303)
                     /locus_tag="P5201_13660"
                     /inference="COORDINATES: protein motif:HMM:NF018513.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0031317 - tripartite ATP-independent
                     periplasmic transporter complex [Evidence IEA];
                     GO_function: GO:0015556 - C4-dicarboxylate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015740 - C4-dicarboxylate transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter large permease subunit"
                     /protein_id="HRV99639.1"
                     /translation="MIELSAEAVTILMLGGVFVLVMTGFPIAFVIGSVAFFTGLAVFG
                     PTVTFHILYSRFYDLSLNYPYLAVPLFTFMGVILQHSGVTKDLYQSLYEAMGRLRGGL
                     AVVTIIFGTILAACLGVIAASVTILTLIALAPMITRGYDKSLASGSIVAAGTLGILIP
                     PSIMLVVYAPQAGLSVGQMFMGAVFPGLLLSACYILYVVIRCRLNPALGPAIPEDQIT
                     PFDAKKMIRLLKSLVPPILLIIAVLGTIFAGIAPPTEAAAVGCFAAVLLAIAYGKFSW
                     GLMRQAMIETMRVSAFVVMIAAMCYAFVGIFMNAGAGDVVAEFILSVPGGKWMSFFVI
                     MAIVFLLGMFIEWIGIVFIVVPIFSPILTALGFNPLWAGMMICINMQMAFQTPPMAMS
                     IFVLKGTAPKELGVTIGDIIWGVIPFILIIMFVLLLCAIFPG"
BASE COUNT          359 a          387 c          416 g          235 t
ORIGIN      
        1 tccccgggaa gatggcgcag agcagaagga cgaacatgat gatgaggatg aacggaatga
       61 cgccccagat gatgtcgccg atcgttactc cgagttcttt cggcgccgtc cccttgagga
      121 cgaagatcga catcgccata ggcggcgtct ggaaggccat ctgcatgttg atgcagatca
      181 tcatgccggc ccaaagcggg ttgaatccca aagctgtcag aatgggcgag aaaataggca
      241 ccacgatgaa gacgatgccg atccactcga tgaacatgcc gagaaggaag acgatcgcca
      301 tgatgacgaa gaaggacatc cacttccctc ccggtaccga gaggatgaac tccgccacga
      361 cgtcgcccgc ccctgcgttc ataaagatgc cgacaaaggc gtagcacatc gccgcgatca
      421 tcacgacgaa agcgcttacg cgcatcgtct cgatcatggc ctgacgcatc agcccccagc
      481 tgaatttccc gtaggctatg gcgaggagaa cggccgcgaa acaccctact gcggcggctt
      541 cagtcggtgg tgcaattccg gcgaagatag tcccgagcac cgcaatgata agcagtatgg
      601 gcggcacaag ggatttgaga aggcggatca tttttttggc gtcgaacggc gttatctgat
      661 cttccgggat cgcgggaccg agcgccgggt tgagcctgca ccggatcaca acgtagagga
      721 tgtagcacgc agagagcagc agccccggga atacggcccc catgaacatc tgcccgaccg
      781 agagtccggc ttgcggcgca tagacgacaa gcatgatgct cggcgggatg aggatgccga
      841 gcgttcccgc cgctacgatg gatcccgatg cgagtgattt atcataccct ctggtgatca
      901 tcggcgcgag ggcgatgagc gtcaggatgg tcacggaagc cgcaataacg cccagacagg
      961 cggcgaggat ggtaccgaag atgatggtca caaccgcaag gccgccccgg agtcgtccca
     1021 tcgcctcgta cagcgactgg tacagatctt tagtaacccc cgagtgttgc agtataaccc
     1081 ccatgaaggt gaagagggga accgccaggt aggggtagtt cagcgacagg tcgtaaaaac
     1141 ggctgtacag gatgtggaac gttaccgttg gaccgaagac tgcaagcccc gtgaaaaagg
     1201 cgacgctccc gatgacgaac gcgatgggga atccggtcat tacaaggacg aagactcctc
     1261 ccagcatgag aatagtgacg gcttcggcgc tgagttcaat caagagtctc accccttacc
     1321 ataaagtaaa aatccctgac gaacttcgct acagcctgaa gcagcagcaa gacgagaccg
     1381 actgcgaaaa tggtccg
//
LOCUS       DAOTIL010000830         1396 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminobacteriaceae bacterium isolate AMR_MDS_3026
            NODE_111532_length_1396_cov_2.722595, whole genome shotgun
            sequence.
ACCESSION   DAOTIL010000830 DAOTIL010000000
VERSION     DAOTIL010000830.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868050
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminobacteriaceae bacterium (wastewater metagenome)
  ORGANISM  Aminobacteriaceae bacterium
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae.
REFERENCE   1  (bases 1 to 1396)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1396)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:19:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,224
            CDSs (total)                      :: 3,198
            Genes (coding)                    :: 3,184
            CDSs (with protein)               :: 3,184
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1396
                     /organism="Aminobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_111532_length_1396_cov_2.722595"
                     /isolate="AMR_MDS_3026"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:3034609"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            276..548
                     /locus_tag="P5201_13665"
     CDS             276..548
                     /locus_tag="P5201_13665"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRV99640.1"
                     /translation="MDGVRVVKHMARLSEWKERIVECRNSGHTVQAWCEQNGINPKTY
                     YRWEESSYQWPRDEAEVQKITFQEYHRLLEGLQVKRRRVHAPKEWG"
     gene            665..>1396
                     /locus_tag="P5201_13670"
     CDS             665..>1396
                     /locus_tag="P5201_13670"
                     /inference="COORDINATES: protein motif:HMM:NF024408.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family transposase zinc-finger binding
                     domain-containing protein"
                     /protein_id="HRV99641.1"
                     /translation="MERPNDVVLSPEAKEYIQFQERQIAELTSQLNYLRKLYFGKKSE
                     KQPLSIPAACDQLSIFDEAEATADSKTEDLPRHTDTAVREHTRAKRRSREEILAGMPA
                     EVHEYDLAPENRGCPHCGHEMEKVGRELIRKECDYIPAHVEVHAYYCATYTCRNCLGG
                     GEGCADCHKPTGACKDCKNGPRTVFVKAPLPPEKRYPVFAHSLASAGTVAYVLYAKYV
                     QAVPLYRRVKDWEMAGLKITRATLAN"
BASE COUNT          356 a          354 c          435 g          251 t
ORIGIN      
        1 gtgatacgga tgaaagccga caaaagctta ttcttagtcc gttgtcttgc gtggatattc
       61 cggttacgaa acctaaatac gccgtctgat cttcaccgta tccctcgaaa aaccgtaagc
      121 gtcaaataac agaatggggg acactggctc caagcagatg agatcatcag atcgtggggg
      181 gatttgagaa acaatgagat tctcggagaa tccaagagaa tcgacgagat tcgtagagac
      241 aaccccacaa tctcatccaa catggaggtg tcgatatgga cggagtccgg gtcgtcaaac
      301 acatggcgcg gctttcggag tggaaggaac gaatcgttga atgccggaac agcgggcaca
      361 cggtgcaggc atggtgcgaa cagaacggga tcaatccaaa gacgtattac cggtgggaag
      421 aaagcagcta ccaatggccg agggacgagg cggaggttca gaagatcact tttcaggaat
      481 atcaccggct gctcgaaggg cttcaggtca agagacgcag agtacatgcg ccaaaagagt
      541 ggggatgaca cagaaattta agaactcttt ttctttgggg catgctccca gcgtgagccg
      601 gtttgacacc ggctccgctt tgtcgtaaca tgagggcagg aagagacgaa gggatgcgcc
      661 ccgtatggag agaccgaatg acgtggtctt gtcgccggaa gcgaaggaat acatccagtt
      721 ccaggagcgg cagatcgcgg aactgaccag tcagctcaat tacctgcgca agctgtactt
      781 tgggaagaag agcgagaagc agcctctttc cattccggcg gcgtgcgacc agttatccat
      841 ctttgacgaa gccgaggcaa cggcggacag caaaacggag gacctgccgc gccatacgga
      901 caccgccgtc cgggagcata cgcgcgcgaa gcggcgctcc cgggaggaaa tcctggcggg
      961 gatgccggca gaggttcacg aatatgatct cgcgccggag aacagaggat gcccccattg
     1021 cggccacgag atggagaagg ttggcaggga actgatccgg aaagagtgcg attatattcc
     1081 cgcgcacgtc gaggttcatg cgtactactg cgccacctac acctgccgca attgcctggg
     1141 cgggggcgaa ggatgcgcgg attgtcacaa acccaccggg gcgtgcaagg actgcaaaaa
     1201 cggcccgcgc acggtgtttg tcaaggcacc tcttccgccg gagaaacggt atccggtctt
     1261 tgcgcactcc ctggcgagcg caggcaccgt cgcctatgtg ctgtatgcca agtatgtgca
     1321 ggccgttccc ctgtaccggc gggtgaagga ttgggagatg gctgggctga agatcaccag
     1381 ggcaacgctg gcgaac
//
LOCUS       DAOTIL010000831         1392 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminobacteriaceae bacterium isolate AMR_MDS_3026
            NODE_112069_length_1392_cov_1.469708, whole genome shotgun
            sequence.
ACCESSION   DAOTIL010000831 DAOTIL010000000
VERSION     DAOTIL010000831.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868050
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminobacteriaceae bacterium (wastewater metagenome)
  ORGANISM  Aminobacteriaceae bacterium
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae.
REFERENCE   1  (bases 1 to 1392)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1392)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:19:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,224
            CDSs (total)                      :: 3,198
            Genes (coding)                    :: 3,184
            CDSs (with protein)               :: 3,184
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1392
                     /organism="Aminobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_112069_length_1392_cov_1.469708"
                     /isolate="AMR_MDS_3026"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:3034609"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..808
                     /locus_tag="P5201_13675"
     CDS             <1..808
                     /locus_tag="P5201_13675"
                     /inference="COORDINATES: protein motif:HMM:NF012515.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="sugar kinase"
                     /protein_id="HRV99642.1"
                     /translation="GAGTRFIQFGGERMGVYFIEKGASQRPSKVIYDRKNTSISAVRP
                     GEFDWETIFEGASAFLFSGITAALSDTMPDVLAEACEKAKEKGVKIFCDLNYRKNLWT
                     PEKAQITMKRLVQYVDVLVGNEEDTDKMLGIKAGESDVHKGELDTASYVDVAKQLQRE
                     YGFEAVGTTLRKSISASDNKWSGMLYTNGTAYLSREYDIRIVDRVGGGDSFTSGLMYA
                     MLNGYDSRKALEFATAASCLKQSIELDFNLVSVEEITALMEGDGSGRVQR"
     gene            864..>1392
                     /locus_tag="P5201_13680"
     CDS             864..>1392
                     /locus_tag="P5201_13680"
                     /inference="COORDINATES: protein motif:HMM:NF012904.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrodipicolinate synthase family protein"
                     /protein_id="HRV99643.1"
                     /translation="MKKLFGVTTSMTTPFDERDRVDVAALEVMTDFSIAKGTDCLYPC
                     GTTGEMHLMSCEERELVAETVVRRAAGRVTVFIHTGAMTTEDTIRLAKHAHAIGADGI
                     GVVTPSYFNVHDRAMVQFFVDVAQSVPADFPVYMYGIPQCAANDLSVEVAAEAARQAP
                     NIVGVKYSYPDMHKIN"
BASE COUNT          305 a          397 c          461 g          229 t
ORIGIN      
        1 cggcgcggga acccggttca tccagttcgg cggcgaacgg atgggcgtct acttcatcga
       61 aaagggggcg tcgcagaggc cctcgaaggt catctacgac agaaaaaata cctcgatttc
      121 ggctgtgcgc ccgggagagt tcgactggga gactattttc gagggagctt cggcctttct
      181 cttctccggc atcaccgcgg cgctgagcga cacgatgccc gacgtgctgg ccgaggcgtg
      241 cgaaaaggcg aaggaaaaag gcgtcaagat cttctgcgat ctgaactaca gaaagaacct
      301 ctggaccccc gagaaggcgc agatcacgat gaagcgcctt gtacagtacg tcgatgtgct
      361 cgtcggcaac gaagaggaca ccgacaagat gctgggcata aaggccggcg aatccgatgt
      421 gcacaagggc gagctggaca cggcgtcgta cgtggacgtc gcgaagcagc ttcagcggga
      481 gtacggcttc gaggcggtgg gcaccacgct ccgcaagtcg atatccgcct cggacaacaa
      541 gtggtccggg atgctgtaca cgaacggaac ggcatatctc tccagagagt acgacatccg
      601 gatcgtcgac agggtgggcg gaggcgattc gttcacgtcc gggctcatgt acgccatgct
      661 caacgggtac gacagcagga aagccctcga attcgcgacg gccgcatcct gcctgaaaca
      721 gagcatcgag ctggatttca acctcgtctc ggtggaggag attaccgccc tgatggaggg
      781 cgacggctcc ggacgagtgc agcgatgaac agttccgcag tgaagcggtt cgccgggaga
      841 gaacccggga agggagatgc gcgatgaaga agctgttcgg agtaacaacg tccatgacga
      901 ctcccttcga cgaaagggac agggtcgacg tggcggccct cgaggtgatg accgatttct
      961 cgatcgccaa aggaacggac tgcctgtatc cgtgcggcac cacgggcgag atgcacctga
     1021 tgtcgtgcga ggagcgggag ctggtcgcgg agacggtggt ccgtcgcgcc gccggccgcg
     1081 tcaccgtctt catccacacg ggcgcgatga ccaccgaaga tacgatacgg ctggcgaagc
     1141 acgcgcacgc catcggcgcg gacggtatcg gcgtcgtgac gccgagctat ttcaacgtcc
     1201 acgacagggc gatggttcaa ttcttcgtcg acgtggcgca gagcgtccct gcggacttcc
     1261 ccgtctacat gtacggcatt ccgcagtgcg cggcgaacga tctgagcgtg gaggtggccg
     1321 ccgaggccgc ccggcaggcg ccgaacattg tcggcgtgaa gtacagctac cccgatatgc
     1381 acaagatcaa cg
//
LOCUS       DAOTIL010000832         1391 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminobacteriaceae bacterium isolate AMR_MDS_3026
            NODE_112188_length_1391_cov_1.782186, whole genome shotgun
            sequence.
ACCESSION   DAOTIL010000832 DAOTIL010000000
VERSION     DAOTIL010000832.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868050
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminobacteriaceae bacterium (wastewater metagenome)
  ORGANISM  Aminobacteriaceae bacterium
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae.
REFERENCE   1  (bases 1 to 1391)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1391)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:19:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,224
            CDSs (total)                      :: 3,198
            Genes (coding)                    :: 3,184
            CDSs (with protein)               :: 3,184
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1391
                     /organism="Aminobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_112188_length_1391_cov_1.782186"
                     /isolate="AMR_MDS_3026"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:3034609"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..262
                     /locus_tag="P5201_13685"
     CDS             <1..262
                     /locus_tag="P5201_13685"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRV99644.1"
                     /translation="VTAAARRWSDAHPGGIALLLLPDPEKEGGAFLLYRGAAVERDFS
                     RFLKSSPSLEARGGGRSDWLNGASSCMDPGVWTQEIERYMSE"
     gene            complement(319..1281)
                     /locus_tag="P5201_13690"
     CDS             complement(319..1281)
                     /locus_tag="P5201_13690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005660413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TAXI family TRAP transporter solute-binding
                     subunit"
                     /protein_id="HRV99645.1"
                     /translation="MNKKFTAGLALLLMVSIFAGAASATTFISIATGGTGGTYYPLGG
                     GIADIISRNVPDFQVTSETGNASAANINLIGTHQIEMAFAQNDIAFWAAKGMSPFKEK
                     YDNIRVVASLYPEHVHCVTLKGSGVSDIMDINGKRVSVGAPGSGVLGDVSAILKVAGI
                     KFSDMQTDFLDFNNTTQRFKDGQLDVGFVVAGYPTSSLMDLATTHDIGLVSFNDEFMS
                     KLVKEFPFFIKDTIPAGTYNGVDQDTPTPAVMAMLVCDAQLSDDVVYKFTKALWDNIA
                     DLHKVHAKAKLITLETALDGVSVPVHPGAAKFYTEKGMKVPAIK"
BASE COUNT          274 a          350 c          451 g          316 t
ORIGIN      
        1 cgtgacggct gcggcgcgca gatggtccga cgcccacccc ggggggatcg cgcttctgtt
       61 gctccccgac ccggagaaag aggggggagc gtttctcctc tacagaggcg ctgcggtaga
      121 gcgtgacttt tctcgcttcc tgaaatcgtc gccctctctt gaagccagag gaggggggcg
      181 aagcgactgg ttgaacggcg cttcgtcctg tatggatccg ggagtgtgga cgcaggaaat
      241 agaacggtat atgtcggaat aaaaaagaag gcggccggag agccgccttc tttcattgag
      301 aacttgggaa aggcgtcgct acttgatagc gggaaccttc atgcccttct cggtgtagaa
      361 cttcgccgcg cccggatgta cggggacgga gacgccgtcg agcgctgttt cgagggtgat
      421 cagcttcgcc ttcgcgtgga ccttgtgcag gtcggcgatg ttgtcccaga gagccttggt
      481 gaacttgtag acgacgtcgt cggaaagctg cgcgtcgcag acgagcatcg ccatgacggc
      541 gggagtcgga gtgtcctgat cgacgccgtt ataggtgccg gcggggatgg tgtccttgat
      601 gaagaagggg aactccttga cgagcttgct catgaactcg tcgttaaagc tgacgaggcc
      661 gatgtcatga gtggtcgcca ggtccatgag ggaggatgtg gggtagccgg cgacgacgaa
      721 accgacgtcg agctgcccat ccttgaagcg ctgggtggtg ttgttgaagt cgaggaaatc
      781 ggtctgcata tcgctgaact tgatccccgc caccttcaga atcgcggata cgtcgccgag
      841 cacgcccgag ccgggagcgc cgacggagac tcttttgccg tttatgtcca tgatgtcgct
      901 cacgccgctc cccttaaggg tgacgcagtg gacgtgctca gggtacaggg aggccacaac
      961 gcggatattg tcatacttct ccttgaacgg agacattccc tttgctgccc agaaggcgat
     1021 gtcgttctgg gcaaaagcca tctcgatctg atgggtgccg ataaggttga tgttggctgc
     1081 ggaagcgttg ccggtttcgg aagtgacctg gaagtcgggg acatttctgc tgatgatgtc
     1141 ggcaatgccg ccgccgaggg gatagtaggt gccgcccgtg ccgcccgtgg cgatggaaat
     1201 gaaggtcgtt gcggacgccg cgcccgcgaa aatggagacc ataagaagaa gagcaagacc
     1261 tgcggtgaat ttcttattca tatgtgtatt cctcctagct tgaatttgtg gtgctttgaa
     1321 tgattcaaaa actcgcgtgt attttgttcc cccctttgta tttgaattga gcgttctctt
     1381 tgcctcctat a
//
LOCUS       DAOTIL010000833         1390 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminobacteriaceae bacterium isolate AMR_MDS_3026
            NODE_112236_length_1390_cov_3.035206, whole genome shotgun
            sequence.
ACCESSION   DAOTIL010000833 DAOTIL010000000
VERSION     DAOTIL010000833.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868050
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminobacteriaceae bacterium (wastewater metagenome)
  ORGANISM  Aminobacteriaceae bacterium
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae.
REFERENCE   1  (bases 1 to 1390)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1390)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:19:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,224
            CDSs (total)                      :: 3,198
            Genes (coding)                    :: 3,184
            CDSs (with protein)               :: 3,184
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1390
                     /organism="Aminobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_112236_length_1390_cov_3.035206"
                     /isolate="AMR_MDS_3026"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:3034609"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..246
                     /locus_tag="P5201_13695"
     CDS             <1..246
                     /locus_tag="P5201_13695"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRV99646.1"
                     /translation="PVQFGERPPAPSRAPEHGQHTEEILMELNIDWDGIAAAKEAGLP
                     FEGLVNEVLKWSEGEVILVKREEHEEEPPPAGETEQI"
     gene            266..>1390
                     /gene="dnaE"
                     /locus_tag="P5201_13700"
     CDS             266..>1390
                     /gene="dnaE"
                     /locus_tag="P5201_13700"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008523239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit alpha"
                     /protein_id="HRV99647.1"
                     /translation="MTRQFVHLHVHTEYSLLDGAIRCGDLAAKAVEFGMPAVAMTDHG
                     AMYGTVEFYDKCTAAGIKPIIGCEVYVDPEGHTSREKRGKNHHLILLAENNEGYRNLV
                     KLCSIAYTDGFYSKPRIDHALLAQYKNGLIVSSACLAGEIPSLILEGREKEALERAFL
                     YRDIVGKENFFLEVMYNAIPEQAVANKALIRMAREHGFPLIATNDAHYLLKEDYDWHE
                     ILLCVQTKSSLDDENRMSFSSNDFYLRSPEEMDSIFGAELPDALDNTVAIAERCNVTL
                     PLGERDYKLPRLDLPEGETLESNLEKEAIVGLSERVGDPVPEEYRKRLDFEIGVINSM
                     GFAGYFLIVAGIIKAAKKRKIPIGPGRGSAAGSLVAWSLKI"
BASE COUNT          310 a          412 c          434 g          234 t
ORIGIN      
        1 ccggtgcaat tcggcgagcg tccgccggcg ccgtcccgcg cgcccgagca cgggcagcac
       61 accgaggaga tcctgatgga gctgaacatc gactgggacg gcatcgcggc ggcgaaggag
      121 gcggggcttc ccttcgaggg ccttgtcaac gaagtgctca agtggtccga gggcgaggtc
      181 atccttgtaa agcgggagga gcatgaagag gagccgcctc ctgcgggaga gacggagcag
      241 atatgaaacg gggaattcca agactatgac aagacagttt gtccatttgc acgttcatac
      301 ggagtacagt cttctcgacg gcgcaatccg gtgcggcgat ctcgccgcca aggccgtcga
      361 attcggcatg cccgccgtgg cgatgacgga ccatggcgcg atgtacggaa ccgtcgagtt
      421 ctacgacaag tgcactgcgg ccggaatcaa gccgatcata gggtgcgagg tctacgtcga
      481 tcccgaaggg cacacatcga gggagaagcg cggaaagaac caccacctga tcctcctggc
      541 cgaaaacaac gaggggtacc gcaacctcgt caaactctgt tccatcgcct acaccgacgg
      601 tttttacagc aagccccgga tagatcatgc gctgctcgcg cagtacaaga acgggctgat
      661 cgtttcgtcc gcctgcctcg ccggggagat cccgtcgctg attcttgagg ggcgtgaaaa
      721 ggaagccctg gagcgcgcgt ttctctaccg ggacatcgtg gggaaggaga acttcttcct
      781 tgaggtcatg tacaacgcca tcccggaaca ggccgtcgcg aacaaggcgc tgatcaggat
      841 ggcgagggag cacggcttcc cgctcatcgc gaccaacgac gcgcactacc tgctgaaaga
      901 ggactacgac tggcacgaaa ttctgctctg cgtccagacg aagagcagcc tcgacgacga
      961 aaacaggatg tcgttctcaa gcaacgactt ctacctccgg tctccagagg agatggactc
     1021 catcttcggc gcggagctcc ccgacgcgct cgacaacacg gtagccatcg cggagcgctg
     1081 caacgtgacc cttcccctgg gagaacgcga ctacaagctc ccgcgcctcg atcttcccga
     1141 gggagaaacg ctcgagtcga acctcgaaaa agaggcgatc gtcggcctga gcgagcgcgt
     1201 cggcgatcct gtacccgagg agtacagaaa gcgtctcgat tttgagatcg gcgtgatcaa
     1261 ttcgatgggg ttcgcgggct acttcctgat cgtcgcgggc atcatcaagg ccgcgaagaa
     1321 gcgaaagatt ccgataggtc cgggacgagg ctccgccgca ggctcgcttg tggcctggag
     1381 cctcaagatc
//
LOCUS       DAOTIL010000834         1390 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminobacteriaceae bacterium isolate AMR_MDS_3026
            NODE_112254_length_1390_cov_2.755056, whole genome shotgun
            sequence.
ACCESSION   DAOTIL010000834 DAOTIL010000000
VERSION     DAOTIL010000834.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868050
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminobacteriaceae bacterium (wastewater metagenome)
  ORGANISM  Aminobacteriaceae bacterium
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae.
REFERENCE   1  (bases 1 to 1390)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1390)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:19:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,224
            CDSs (total)                      :: 3,198
            Genes (coding)                    :: 3,184
            CDSs (with protein)               :: 3,184
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1390
                     /organism="Aminobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_112254_length_1390_cov_2.755056"
                     /isolate="AMR_MDS_3026"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:3034609"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..519
                     /locus_tag="P5201_13705"
     CDS             <1..519
                     /locus_tag="P5201_13705"
                     /inference="COORDINATES: protein motif:HMM:NF018513.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter large permease subunit"
                     /protein_id="HRV99648.1"
                     /translation="FVYGDLRWKRIPEVFASAARNTAGVMFIIAVASFLSWILTSRQI
                     PQTIARELLSLSTNPVIVLLLLNAFILFLGCFIDAVSILVLISPVALPIIRSLGIDPV
                     YFGVLLVLNICLGALTPPVGTCLFVASSVGGVNLTKTSRAILPIVLLAVFCLALAIFF
                     PAVILFLPSLLR"
     gene            613..1302
                     /locus_tag="P5201_13710"
     CDS             613..1302
                     /locus_tag="P5201_13710"
                     /inference="COORDINATES: protein motif:HMM:NF019310.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HRV99649.1"
                     /translation="MSTERASYGKSFWISMAVIFLTQAFLAVFMLYPSVLRDQGFSLG
                     LSGWLMSVFNLCSTLGRPLGARATERLGIRTTLLWTSGLVAVFTMPLAFTEHPLLLLF
                     LRGTGGVFWGISMVAISSYQGLSIPAARRGSAYAWIAVAYVFPQVTLFPLADWFASSR
                     MLTAYFLLAAAVEAAIVLVAAPLPPLRRAESSQEEPKEWGRWSELPSLPGFRAVLATM
                     FLFAFVNASTL"
BASE COUNT          208 a          477 c          370 g          335 t
ORIGIN      
        1 tttgtctacg gcgatcttcg ctggaagcgg attccggagg tattcgcctc tgcggccagg
       61 aataccgcgg gcgtcatgtt catcatcgcc gtggcctcct tcctgagctg gattctcacc
      121 agccggcaga tcccccagac aatcgccagg gaactgctgt cgctttctac aaacccggtg
      181 atcgtactgc ttctgttgaa cgcgttcatc ctcttcctgg ggtgtttcat cgacgcggtc
      241 tccatcctcg tgctcataag ccccgtcgcg ctgccgatca tccgctctct cggaatcgac
      301 ccggtctact tcggcgttct gctggtgctc aacatctgtc tcggcgccct cacgcccccg
      361 gtgggaacat gtctcttcgt cgcctcgagc gtcggcggcg tcaacctcac aaagacgagc
      421 agagccatac tgcctatcgt cctgctcgcg gtattctgcc tggcgctggc cattttcttt
      481 cctgctgtta tactcttctt gccctcgctg ttgcggtgat atccctaaaa acacggcggt
      541 tcgcacccat gtcgcgaaat ctgcggaccg cctgcgctgc tccgtacttc gaagcagagc
      601 aaaggagaga ctatgtccac tgaacgcgct tcttacggaa aatctttctg gatctcgatg
      661 gcggtgatct ttcttacgca ggcgttcctc gccgtcttca tgctctaccc ctccgtgctc
      721 cgcgaccagg ggttctctct cggcctctcg ggatggctga tgagcgtgtt caacctctgc
      781 tccacgctgg gcaggcctct gggagcgcga gcgacggaac ggctcgggat ccgcaccacg
      841 ctgctctgga cctccgggct cgtcgccgtc ttcacaatgc cgctcgcctt cacggaacac
      901 ccgctgctgc ttctcttcct ccggggaaca ggcggcgtct tctgggggat ctctatggtt
      961 gctatctcct cataccaagg gctcagcatc cccgcagcgc ggagagggag cgcgtacgca
     1021 tggattgccg tggcgtatgt ctttcctcag gtcactcttt tccctctggc ggactggttt
     1081 gcttcctccc gcatgctcac agcctacttc ctgctcgccg cagcggtcga agccgccatc
     1141 gtcctggtgg ccgcgccgct tcccccgctt cgacgagcgg agagttcgca agaggaaccg
     1201 aaagagtggg ggagatggag cgaactcccc tcgctccccg gattccgggc ggtgctcgcc
     1261 accatgttcc tctttgcctt cgtcaacgcc tccacactct agtaccttcc ggccttcctc
     1321 tccatgagag ggtactcgcc gagcgtcttc atcgttccta acgcgatgac cgcgatcgcc
     1381 ctgcgcctcg
//
LOCUS       DAOTIL010000835         1389 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminobacteriaceae bacterium isolate AMR_MDS_3026
            NODE_112363_length_1389_cov_3.131184, whole genome shotgun
            sequence.
ACCESSION   DAOTIL010000835 DAOTIL010000000
VERSION     DAOTIL010000835.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868050
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminobacteriaceae bacterium (wastewater metagenome)
  ORGANISM  Aminobacteriaceae bacterium
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae.
REFERENCE   1  (bases 1 to 1389)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1389)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:19:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,224
            CDSs (total)                      :: 3,198
            Genes (coding)                    :: 3,184
            CDSs (with protein)               :: 3,184
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1389
                     /organism="Aminobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_112363_length_1389_cov_3.131184"
                     /isolate="AMR_MDS_3026"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:3034609"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(240..434)
                     /locus_tag="P5201_13715"
     CDS             complement(240..434)
                     /locus_tag="P5201_13715"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRV99650.1"
                     /translation="MEENNKLFCGKCMAEMLPAPTGFSYLGHAFRTDVLRCPSCGQVF
                     IPESLAKGRMAEVETTLEDK"
     gene            complement(421..>1389)
                     /locus_tag="P5201_13720"
     CDS             complement(421..>1389)
                     /locus_tag="P5201_13720"
                     /inference="COORDINATES: protein motif:HMM:NF014774.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(Fe-S)-binding protein"
                     /protein_id="HRV99651.1"
                     /translation="LLGLEPGTALRLGCCGAPAVWAGDMELFRQVRERILDDWRALGS
                     PQMVMACPTCLEMFARYIPEIDCAPLVDVLAASGHPPAEQGNGEIVSVFDPCASRNRP
                     ESQRAARSLVRGAGYELAPLPYEGKRAQCCSYGGQIDIAAPKYARRLAEQRSKDGEAP
                     YVVYCSNCRDVFAHAGKPARHILDIFFGLNGWNAAPPTFDERRKRREAMKNDLVQEFW
                     PDMELRGKEEIMDNRKSIAIAPELREKMNRERLLEEDALAVIEECERSGRRVWDPSAE
                     RFFGCKEIGSMTQWVEYAPSPEGYALFNTYAHRMKIEREDAPHGRK"
BASE COUNT          213 a          460 c          438 g          278 t
ORIGIN      
        1 gcagcgcgcg gccgcgtggt gcgacgccgc cctcgtgacg ggctccacgc tctcgaacgg
       61 gacgatcgac gcgctccttg acgcgttctc cgggaagcgt atcgtctttt tcggaacgac
      121 gatcgcagtc cctgccgagc ttctgggctt cgagaggctc tgtttcgagg cggagtaaag
      181 cgtgttccgg aggtgaagag gccgcgccgc tgtggttgtg ctgtctcttc acctccgttt
      241 tatttgtctt cgagcgtcgt ctccacttcg gccatgcgcc ccttggcgag gctctccggg
      301 atgaagacct gtccgcacga cgggcagcgc agcacgtccg tgcggaaggc atgcccaagg
      361 tagctgaacc cggtcggggc ggggagcatc tccgccatgc acttcccgca gaagagttta
      421 ttattttctt ccatgagggg cgtcctcccg ctcgatcttc attctgtgcg cataggtgtt
      481 gaagagcgcg tacccttcgg gggacggcgc gtactccacc cactgcgtca tggagccgat
      541 ctccttgcag ccgaagaagc gttccgcgga cggatcccat actctgcggc cgcttctctc
      601 gcactcctcg atcacggcga gcgcgtcctc ctccagaagc cgctcgcggt tcatcttctc
      661 ccgcagttcg ggagctatgg cgatactttt tcgattgtcc atgatctcct cttttccccg
      721 gagttccata tcgggccaga actcctgaac aagatcattt ttcatggcct cgcgccgttt
      781 tctgcgctcg tcgaacgtcg gcggcgcggc gttccatccg ttcagcccga agaagatgtc
      841 gagaatatga cgcgccggct tccccgcgtg cgcgaagacg tcgcggcagt tggagcagta
      901 gacgacgtac ggcgcttcgc cgtccttgct tctctgctcc gccagccggc gcgcgtactt
      961 cggcgcggcg atgtcgatct gcccgccgta ggagcagcac tgcgcccgct ttccctcgta
     1021 ggggagggga gcgagttcgt accccgcgcc gcggacgagc gagcgcgccg cacgctgcga
     1081 ttcaggtctg ttgcggctcg cgcacggatc gaagacggag acgatctccc cgttcccctg
     1141 ctccgcggga ggatgtccgg atgcggcgag gacgtcgacg agcggcgcgc agtcgatctc
     1201 cggaatgtag cgggcgaaca tctccaggca tgtcgggcag gccattacca tctgcggact
     1261 gccgagcgcg cgccagtcgt ccagaatacg ctcccgcacc tgccggaaaa gctccatgtc
     1321 gcccgcccag acggcgggcg ctccgcagca tccaagccgg agggcggtgc cgggctcgag
     1381 gccgagcag
//
LOCUS       DAOTIL010000836         1388 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminobacteriaceae bacterium isolate AMR_MDS_3026
            NODE_112448_length_1388_cov_4.505626, whole genome shotgun
            sequence.
ACCESSION   DAOTIL010000836 DAOTIL010000000
VERSION     DAOTIL010000836.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868050
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminobacteriaceae bacterium (wastewater metagenome)
  ORGANISM  Aminobacteriaceae bacterium
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae.
REFERENCE   1  (bases 1 to 1388)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1388)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:19:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,224
            CDSs (total)                      :: 3,198
            Genes (coding)                    :: 3,184
            CDSs (with protein)               :: 3,184
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1388
                     /organism="Aminobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_112448_length_1388_cov_4.505626"
                     /isolate="AMR_MDS_3026"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:3034609"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            143..466
                     /locus_tag="P5201_13725"
     CDS             143..466
                     /locus_tag="P5201_13725"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRV99652.1"
                     /translation="MKNSTLRDPYLSIWFCQRKTDHPYTHVREVAKTAGIAVKNFYDR
                     THRYLTSLYSRPWMLKHATDAVLNGIIVEGVLQPTLFYAGALSIIGFIEEQNAVEKMI
                     REKLR"
     gene            complement(928..>1388)
                     /locus_tag="P5201_13730"
     CDS             complement(928..>1388)
                     /locus_tag="P5201_13730"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRV99653.1"
                     /translation="LLSYGDALGALFYLANPSKTEGFLEAAERLRIFLLGLSDEEREL
                     TTNHLRGYLRILMKKEGLEIDDALDLCLEREEADVMLTYMQKEFRKARKEGREEGREE
                     GREEGREKGQLEMGISIALAMLEAGEPEEKILRYTGLSLSQLEEIRDGKK"
BASE COUNT          329 a          355 c          320 g          384 t
ORIGIN      
        1 cgtttgcccg aagtgcgctc tccgctccgt cgtaagtggt cgctcgtccg ggataccctt
       61 tacccgtagt taacccggcg ccccgcacga agcgctccgc aagggcacac ggaagcggaa
      121 cgcaatcaag ggattcggta atatgaaaaa cagcacattg cgagatccgt atctttccat
      181 ctggttttgt caacgcaaaa ctgaccaccc ttatactcat gtcagggaag tcgctaaaac
      241 ggctggtatt gctgttaaaa atttttatga caggacacat cgctatctaa cgagccttta
      301 ttcaagacca tggatgttga agcacgcaac agacgcggtc ctcaacggta ttattgtaga
      361 gggcgttttg cagccgacct tattctatgc cggagcgctg agtatcatcg gttttatcga
      421 ggaacaaaac gctgttgaga aaatgattcg agaaaaactg agatagtttt ttattttttt
      481 cgtgaggaga agtacaatga gctgtctttt taaaaaaatt taccagtata aattttattt
      541 tattgttgat gtgtggcgcc ttttgcattc agcctagtta cgcatatgcc tcgaaaattg
      601 acactgtatt ttccggaata atagaaaatt cgtttagaaa agctgggaaa aacgtcgatg
      661 atcttgccgc attgaggcga ggaggtgcat tcagcgctct tctgcaaaaa aacaataact
      721 tcatgaatgt atttcggaaa gttcttgaca atgatgtact tgctcttaaa aaaatagcgt
      781 ttcctgctgg tgcaagatat atcgatgatc ttatggcgct ttctcttcgt cgatagcatc
      841 gtcgaagcat gcagcaaact cccggagata gtcgatcaac ggtctccgaa gggcttgctc
      901 gtcgagtcct cctgtcttgg tcggcgatca ctttttgccg tcgcgtattt cctccaactg
      961 tgagagggaa aggccggtgt agcggagaat cttctcctcg ggttcgccgg cttcgagcat
     1021 cgccagagcg atggagattc ccatttcaag ctgtcccttc tctcgtcctt cttctcgtcc
     1081 ttcttctcgt ccttcttctc gcccttcctt ccgcgccttt ctaaactctt tttgcatgta
     1141 ggtcagcatc acgtcagcct cctcccgttc caggcagagg tccaaagcat cgtctatctc
     1201 cagaccttcc ttcttcatca ggattctcaa gtaccctcgc agatgatttg tcgtcagctc
     1261 ccgctcttcg tccgaaaggc caagtagaaa tatccgcagc cgctccgccg cctccagaaa
     1321 gccctccgtc ttcgagggat tcgccaggta gaagagcgcg cccagggcgt ctccgtagga
     1381 gagaagcg
//
LOCUS       DAOTIL010000837         1388 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminobacteriaceae bacterium isolate AMR_MDS_3026
            NODE_112463_length_1388_cov_3.255814, whole genome shotgun
            sequence.
ACCESSION   DAOTIL010000837 DAOTIL010000000
VERSION     DAOTIL010000837.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868050
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminobacteriaceae bacterium (wastewater metagenome)
  ORGANISM  Aminobacteriaceae bacterium
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae.
REFERENCE   1  (bases 1 to 1388)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1388)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:19:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,224
            CDSs (total)                      :: 3,198
            Genes (coding)                    :: 3,184
            CDSs (with protein)               :: 3,184
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1388
                     /organism="Aminobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_112463_length_1388_cov_3.255814"
                     /isolate="AMR_MDS_3026"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:3034609"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..513)
                     /locus_tag="P5201_13735"
     CDS             complement(<1..513)
                     /locus_tag="P5201_13735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009165726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deacylase"
                     /protein_id="HRV99654.1"
                     /translation="MFDYEKYKTPRKVVLLLLALALAGFGAREFTELRTMKETVVVSD
                     AFTKMVMLSDYFPPIKGTGVDTPVYIYDSGVPGGSMFYLGGTHPYEPATMLSAYVMME
                     NLKVEKGRVFICPHANMSASTVGMLGNAYPKFLHVETAFGPKPYRIGDRNTSPSEITG
                     PTRSFVPSGPT"
     gene            complement(525..710)
                     /locus_tag="P5201_13740"
     CDS             complement(525..710)
                     /locus_tag="P5201_13740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008521714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRV99655.1"
                     /translation="MEELLRVSNEIIHQIYFVLAGLCGLVLLRGLFSRNTRKTIVYDI
                     VYAYTIIPFLLRALHIK"
     gene            complement(731..>1388)
                     /locus_tag="P5201_13745"
     CDS             complement(731..>1388)
                     /locus_tag="P5201_13745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008521713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="citrate transporter"
                     /protein_id="HRV99656.1"
                     /translation="AHERMNWFRIAAPLLVFVVLILLSKYAAFSTPVLGIPLIFIIST
                     AVAMLVSPVRKGALHWYGVLANTSEQVFPLLATVISVGVLVNIMTATGVRGLIAITFV
                     TLPVYLIYAFALVVLPFAQGSLSYSSGIILGTPLIFLFNSVGVNVTIVATALSLIFPL
                     GDCLPPSRISGRVAIEVSGYQGTYVSFLRAIFVPALFMGLVALGMLMYANHFRWLIVY
                     "
BASE COUNT          297 a          392 c          453 g          246 t
ORIGIN      
        1 ggtcgggccg gacggcacga agctgcgcgt cggcccggtg atctcgctcg gcgaggtgtt
       61 cctgtcgccg attctgtacg gcttggggcc gaacgccgtc tccacgtgga ggaacttcgg
      121 gtaggcgttc cccagcattc cgacggtgga ggcgctcata ttggcgtggg ggcagatgaa
      181 gacacgtccc ttctccacct tgaggttctc catcatgacg taggcggaga gcatcgtggc
      241 gggctcgtag gggtgcgtcc cgccgaggta gaacatcgaa ccgccgggca cgcccgagtc
      301 gtagatgtag accggcgtgt ccactcccgt ccccttgatg ggagggaaat agtcgctgag
      361 catgaccatt ttggtgaaag cgtccgagac gacgaccgtc tccttcatcg tgcgcagctc
      421 cgtgaactcg cgtgctccaa agcccgcaag cgcgagcgcg agcagcagca ggacgacctt
      481 ccggggcgtt ttgtattttt cgtagtcgaa cattcgccgt caccctactt tatgtgcagc
      541 gcgcgcagca gaaagggaat gatcgtgtag gcgtagacga tgtcgtacac gatggtcttg
      601 cgcgtgttgc gggagaatag cccccgaagg aggacaaggc cgcacagtcc ggccaggacg
      661 aaatagatct gatggatgat ctcgttggat acgcgaagaa gctcttccat aatccagact
      721 cctctctttt ctaatacacg atgagccatc ggaagtgatt ggcgtacatc agcattccga
      781 gcgcgacgag ccccatgaag agcgcgggga cgaagatcgc cctgaggaac gagacgtacg
      841 tcccctggta ccccgatact tctatggcga ccctgccgga gatcctcgac ggcggcagac
      901 agtcgcccag cgggaaaatg aggctgaggg ccgtggccac gatggtcacg ttgacgccga
      961 cggagttgaa gaggaagatc agcggcgttc ccaggatgat tccgctgctg tagctgagcg
     1021 acccctgcgc gaaaggaagc accacgagag cgaaggcgta aatcaaatac accggcagcg
     1081 tgacgaaggt gatggcgatg agcccccgga cgccggtcgc agtcattatg ttcaccagga
     1141 caccgacgct gatgacggtc gcgagcagcg gaaagacctg ctccgatgtg ttggcgagca
     1201 cgccgtacca gtggagcgct cccttccgca cgggtgagac gagcatggcg acggcagtgc
     1261 tgatgatgaa gatgagcggg attccgagca ccggcgtcga gaaggccgcg tacttggaga
     1321 gcagaatcag caccacgaag accagcagcg gcgcggcgat acggaaccag ttcatccgtt
     1381 cgtgcgct
//
LOCUS       DAOTIL010000838         1387 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminobacteriaceae bacterium isolate AMR_MDS_3026
            NODE_112579_length_1387_cov_2.801802, whole genome shotgun
            sequence.
ACCESSION   DAOTIL010000838 DAOTIL010000000
VERSION     DAOTIL010000838.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868050
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminobacteriaceae bacterium (wastewater metagenome)
  ORGANISM  Aminobacteriaceae bacterium
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae.
REFERENCE   1  (bases 1 to 1387)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1387)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:19:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,224
            CDSs (total)                      :: 3,198
            Genes (coding)                    :: 3,184
            CDSs (with protein)               :: 3,184
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1387
                     /organism="Aminobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_112579_length_1387_cov_2.801802"
                     /isolate="AMR_MDS_3026"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:3034609"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1256)
                     /locus_tag="P5201_13750"
     CDS             complement(<1..1256)
                     /locus_tag="P5201_13750"
                     /inference="COORDINATES: protein motif:HMM:NF033578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS5 family transposase"
                     /protein_id="HRV99657.1"
                     /translation="MDVYKSPVGQPKLKFENISFEFTPDPSNRWVRLADLIPWEELEL
                     LSGYRNHFGSTGNPALPFRVAFGALLVQARLKLTDEETVEQIRENPYIQYFLGFEGYR
                     SEKKPFDPSMMVYFRKRLDTETLKALDLRIFERRRELEEIAKKAAEEAAKKGKKEEEG
                     SSVPEPESAPVDGSDEETPPSASPETPCGSNAPTPEGAPQESPSPAKGDAAPKGTLIL
                     DATCAPADITYPTDLKILSAARIATEKLIDAVWERIGKPGEKKPRTYRRIAARNAVVA
                     CKQRNIGGKKLRSALKKQLSWLARNLNSIEDLLASSAYGENLLNERERKTLETAREVH
                     RQQKHMLDTRTHSAERRIVNFAQPHVRPIVRGKAGAKVEFGAKILVAVENGHTFVVHS
                     SWENFNESTLFVEAVEQYRERNGCWP"
BASE COUNT          207 a          415 c          407 g          358 t
ORIGIN      
        1 tccggccagc atccgttccg ttctcggtac tgctcgaccg cctcgacgaa gagcgtgctc
       61 tcgttgaagt tctcccacga cgaatggacg acgaaggtgt gtccgttctc cacggcgacg
      121 aggatcttcg ctccgaattc gactttcgct cccgctttgc ccctcactat cggccggacg
      181 tgcggctggg cgaagttgac tattctccgt tccgcgctgt gcgtccgggt gtccagcatg
      241 tgcttctgct gtcggtgcac ctcccgcgcg gtttcgagcg tcttccgttc ccgttcgttc
      301 aggaggttct ctccgtatgc gctcgaggca agcaggtcct cgatggagtt caggtttctc
      361 gcaagccagg agagctgctt cttgagggcg ctccggagct tcttcccgcc gatgtttctt
      421 tgtttgcagg caacgactgc gttcctggcg gcgatgcggc ggtaggtacg gggcttcttc
      481 tctccaggct tcccgattcg ttcccagacc gcgtcgatga gtttctccgt ggcaatcctg
      541 gcggcagaaa gtatcttgag gtcggtggga taggtgatgt cggcaggagc acaggtggcg
      601 tccaggatga gcgttccctt gggggcggcg tctcccttcg cgggagacgg gctctcctgc
      661 ggggctcctt ccggagtcgg agcgttgctt ccgcagggtg tctccggact tgccgagggc
      721 ggtgtttctt cgtcgctccc gtcaaccggg gccgattccg gttccggtac ggaagagccc
      781 tcttcttcct tcttcccctt cttcgccgcc tcttccgcag ctttcttcgc gatctcttcc
      841 aattctctcc gtctctcgaa gatgcgaaga tccagggcct tgagcgtttc agtatccaga
      901 cgtttcctga agtacaccat catggacggg tcgaacggtt tcttttccga gcggtagcct
      961 tcgaagccga ggaagtactg gatgtacggg ttctcccgaa tctgctcgac cgtctcttcg
     1021 tcggtgagct ttagcctcgc ctgcacgaga agcgccccga aggcgacgcg gaaggggagc
     1081 gccgggttgc cggtgctccc gaagtgattc cggtagccgg agaggagttc gagctcctcc
     1141 cacgggatga ggtcggcgag acggacccat ctgttgctcg ggtccggtgt gaattcaaac
     1201 gaaatgttct cgaattttaa tttcggctgg ccgaccggcg acttgtacac atccatccca
     1261 cgctctcagc aaggttttcg gggctcatat accctttttc ctgcaccatt ttaccgtgaa
     1321 aaccgcttct tttccagttc tgacaaggct ttacgggttg ttcaggatgc cctaatcagt
     1381 tttgatg
//
LOCUS       DAOTIL010000839         1387 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminobacteriaceae bacterium isolate AMR_MDS_3026
            NODE_112594_length_1387_cov_2.540541, whole genome shotgun
            sequence.
ACCESSION   DAOTIL010000839 DAOTIL010000000
VERSION     DAOTIL010000839.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868050
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminobacteriaceae bacterium (wastewater metagenome)
  ORGANISM  Aminobacteriaceae bacterium
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae.
REFERENCE   1  (bases 1 to 1387)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1387)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:19:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,224
            CDSs (total)                      :: 3,198
            Genes (coding)                    :: 3,184
            CDSs (with protein)               :: 3,184
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1387
                     /organism="Aminobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_112594_length_1387_cov_2.540541"
                     /isolate="AMR_MDS_3026"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:3034609"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1064)
                     /locus_tag="P5201_13755"
     CDS             complement(<1..1064)
                     /locus_tag="P5201_13755"
                     /inference="COORDINATES: protein motif:HMM:NF019310.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HRV99658.1"
                     /translation="MSSKTFNLLRPESSGFKLSLLSTGHFFNDLYASFLPTFIPTIIS
                     RLGITMAQAGFFSTFLGIIHIIFQPIIGYLSDRSANPWLVIWGPIMTCLGATMIPLSP
                     SYGAALFFVGLWALGSAMFHPQGHGGVGHVVPREKLTVSLALFAVAGTAGVTVSPLFA
                     VALVNTVGFRLMPVVAIIPVLLLGLFTWRTMPSISHESGDAMTPQKGLLATLKSVFVV
                     IYPVWALSTVRDAASQGVRMFLPIRIASEGGEIGMVGTVLFLIMLSSTISMVLIGKIA
                     DRWGKKRVLLGVMALTPLSLLAGVYTENWVSIFCFLIGTSAINATLPVTAAIAQEMVP
                     NSRGMASSIVMGLSWGFGNML"
     gene            1144..1311
                     /locus_tag="P5201_13760"
     CDS             1144..1311
                     /locus_tag="P5201_13760"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRV99659.1"
                     /translation="MKIHNAKTAFAEGRFYSNGKTIESGILIRLSRPFSEKTSKKFFD
                     LIVKVKGIDIP"
BASE COUNT          393 a          365 c          372 g          257 t
ORIGIN      
        1 agcatgttgc cgaatcccca tgagagcccc ataacaatgg agctcgccat accgcgggag
       61 ttggggacca tctcctgcgc tatggcggcc gtgacgggga gcgtggcatt gattgcggaa
      121 gttcctataa gaaagcaaaa aatactcacc cagttctcgg tgtacacgcc tgcaaggaga
      181 gagagcgggg tgagcgccat cacacccagc agcactctct tcttccccca cctgtcggcg
      241 atcttgccga taaggaccat gcttatcgta ctcgagagca tgatgaggaa aaggacagtg
      301 cccaccatgc cgatctcccc tccttcggag gcgatacgaa tgggcagaaa catacggacg
      361 ccctggctcg ccgcgtcgcg gacagtagag agagcccata cagggtagat gacgacgaag
      421 acggacttca gcgtcgccag cagccccttc tgcggcgtca tagcgtcgcc gctctcatgc
      481 gaaatcgacg gcattgtcct ccaggtgaac aagccgagta gaaggacagg gattattgcc
      541 accacgggca tcaggcgaaa acctactgtg tttaccaagg ccaccgcgaa gagcgggctg
      601 accgtaacac ccgccgtccc ggcaactgcg aagagagcca aagagacagt aagtttttcc
      661 cggggaacga cgtgccctac cccgccgtgc ccctgcgggt ggaacatagc gcttccaagc
      721 gcccagagtc cgacgaaaaa gagcgccgcc ccgtaggacg gagaaagcgg gatcatcgtc
      781 gccccgaggc acgtcatgat cggcccccag ataacgagcc aggggttcgc cgagcggtcg
      841 gagagatacc ctatgatcgg ctgaaagata atatggataa ttccgaggaa agtgctgaaa
      901 aagcccgcct gcgccatcgt aatccccagc ctggagatga tcgtcgggat gaacgtaggc
      961 aaaaaagacg cgtagaggtc attgaaaaag tgccccgtcg agagcagcga aagcttgaaa
     1021 cccgaactct cggggcgcaa cagattaaaa gtttttgacg acataaaaca aactcactcc
     1081 ttgtgattat actttaagct aaatactatc atacaccatg cagaatgtaa tagggggagg
     1141 aaggtgaaaa tacataatgc aaaaacggcc tttgcggaag gccgttttta cagcaacgga
     1201 aagacgatcg aaagtggaat tttaatcagg ctttcaagac ctttcagcga aaaaacctca
     1261 aaaaagttct ttgatcttat cgtcaaagtt aaaggaattg atataccatg atccatcggg
     1321 attatagaaa acaaaaatcc agcgcatcgc gtggtattta tatttctgaa ggtggacgat
     1381 ccgcatc
//
LOCUS       DAOTIL010000840         1385 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminobacteriaceae bacterium isolate AMR_MDS_3026
            NODE_112803_length_1385_cov_2.772932, whole genome shotgun
            sequence.
ACCESSION   DAOTIL010000840 DAOTIL010000000
VERSION     DAOTIL010000840.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868050
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminobacteriaceae bacterium (wastewater metagenome)
  ORGANISM  Aminobacteriaceae bacterium
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae.
REFERENCE   1  (bases 1 to 1385)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1385)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:19:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,224
            CDSs (total)                      :: 3,198
            Genes (coding)                    :: 3,184
            CDSs (with protein)               :: 3,184
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1385
                     /organism="Aminobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_112803_length_1385_cov_2.772932"
                     /isolate="AMR_MDS_3026"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:3034609"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            65..595
                     /locus_tag="P5201_13765"
     CDS             65..595
                     /locus_tag="P5201_13765"
                     /inference="COORDINATES: protein motif:HMM:TIGR00632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="very short patch repair endonuclease"
                     /protein_id="HRV99660.1"
                     /translation="MTDTLSEKKRSETMSSIRSADTKPEWILRCGLHRMGFRYRLRDK
                     KLPGTPDLVFPKYRTVVFVHGCFWHRHEGCRKSTFPKSRQAFWAEKFAGNVERDARTR
                     AELERAGWRVIVVWECELEGDTEATLERTARLLRAPSDAEQAIPPPRRELLDIASEKV
                     RLRVASYKAGKKPAEE"
     gene            complement(669..>1385)
                     /locus_tag="P5201_13770"
     CDS             complement(669..>1385)
                     /locus_tag="P5201_13770"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRV99661.1"
                     /translation="APSGKDGWGRRIGVVEAASKKATVLKTIPGENSYGPRWSPDGKS
                     LLFNHWDERSSDWVFGVATIADGSFRVLAPEQKGIYSPFWSADGASVYGQDLDTLYRI
                     DAESGAVVERRPLASVTGGEAMVSSALRFSLSPDGTMWLFDAEVEDTGKLMKHGEPLN
                     SAVFLHTPADGKTRRLTPETICAMHPSWLPGGREFLFAGYGPKEAKRKGSPFAIFRRG
                     FDGTKTTLLIKDGDSPSAGL"
BASE COUNT          245 a          464 c          425 g          251 t
ORIGIN      
        1 aggacgtcta agaattgaac tgcggaatag ctcctgatat acctcgtccg gcggcgctcg
       61 ccgcatgacc gacacactct ccgagaagaa gcgctccgag acgatgtcct ccatccggag
      121 cgcggatacg aagcctgagt ggatactccg ttgcggactc caccggatgg ggttccgcta
      181 ccgtctgcgc gacaaaaaac ttcccggaac acccgacctc gtcttcccga agtaccggac
      241 ggtcgtcttc gtgcacggct gcttctggca caggcacgaa ggatgccgga agtcgacctt
      301 tcccaagagc aggcaggcat tctgggcgga gaagttcgcc ggcaacgtcg agcgcgacgc
      361 gcggacgcgg gcggaactcg aacgtgcggg gtggcgcgtc atcgttgtct gggagtgcga
      421 actcgagggg gacacggagg cgacgctcga acgcacggcc cgattgcttc gcgcgccgtc
      481 cgacgcggaa caggcgattc caccgccgcg cagagagctg ctcgatatcg cttccgaaaa
      541 ggtccgcctc cgcgtcgctt cgtacaaggc cgggaaaaaa ccggcggagg agtgatatct
      601 gcggttgctt ccgggttctc ctgtccggtt aaacagcacg gaaccccggt tgcattgtgc
      661 ggtctttttt acagccccgc cgatggactg tctccatctt ttatcaagag cgttgtcttc
      721 gtcccgtcga atccacgccg gaagatcgcg aagggcgagc ccttccgctt cgcctccttc
      781 ggcccgtacc ctgcgaagag gaactccctc cctccgggaa gccacgaggg gtgcatcgcg
      841 cagatcgtct ccggcgtcag ccgccgcgtc ttcccgtccg ccggggtatg cagaaagacc
      901 gccgagttca gcggctcgcc gtgtttcatc agctttcctg tgtcctccac ctcggcgtcg
      961 aagagccaca tcgtcccgtc cggcgagagg gagaatcgga gggcgctcga caccatcgcc
     1021 tcgccgccgg tgaccgaagc gagcggacgc cgctcgacga ccgcgccgct ttcggcgtcg
     1081 atccggtaga gcgtgtcaag gtcctgccca tagacggacg ctccgtccgc agaccagaag
     1141 ggggagtaga tccccttttg ctccggcgcg aggacgcgga agctcccgtc cgctatcgtc
     1201 gctaccccga acacccagtc ggaactccgc tcgtcccagt ggttgaagag gagcgacttc
     1261 ccgtccggcg accagcgcgg gccgtagctg ttctcgccgg ggatcgtctt caggacggtt
     1321 gctttcttcg acgccgcctc gacaacgccg attctccgtc cccacccgtc ctttcctgag
     1381 ggagc
//
LOCUS       DAOTIL010000841         1384 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminobacteriaceae bacterium isolate AMR_MDS_3026
            NODE_112904_length_1384_cov_3.376975, whole genome shotgun
            sequence.
ACCESSION   DAOTIL010000841 DAOTIL010000000
VERSION     DAOTIL010000841.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868050
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminobacteriaceae bacterium (wastewater metagenome)
  ORGANISM  Aminobacteriaceae bacterium
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae.
REFERENCE   1  (bases 1 to 1384)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1384)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:19:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,224
            CDSs (total)                      :: 3,198
            Genes (coding)                    :: 3,184
            CDSs (with protein)               :: 3,184
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1384
                     /organism="Aminobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_112904_length_1384_cov_3.376975"
                     /isolate="AMR_MDS_3026"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:3034609"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..183
                     /locus_tag="P5201_13775"
     CDS             <1..183
                     /locus_tag="P5201_13775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000879771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CopG family transcriptional regulator"
                     /protein_id="HRV99662.1"
                     /translation="DRLAKETGRTKAFYIRKMIEDRIDDMEDYYLAAERLAQSRKFGS
                     RSLTSEEARRELGLED"
     gene            167..433
                     /locus_tag="P5201_13780"
     CDS             167..433
                     /locus_tag="P5201_13780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000262326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008657 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) inhibitor activity
                     [Evidence IEA];
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA];
                     GO_process: GO:0030541 - plasmid partitioning [Evidence
                     IEA];
                     GO_process: GO:0051290 - protein heterotetramerization
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HRV99663.1"
                     /translation="MAWRIEYDPSALGDLRHIDRRMAQRIVDYMDRRVAMMDNPRTLG
                     KALKGGFGDFWRYRVGDYRVICSIEDKNVMVLVVRVGHRSDVYR"
     gene            496..1302
                     /locus_tag="P5201_13785"
     CDS             496..1302
                     /locus_tag="P5201_13785"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRV99664.1"
                     /translation="MRRCLYSTVFSLFLLFSAPAAAVDFTGIWTFSKEDGTEYSFDLV
                     QRDTALEGTFSTYDPLLDKYGEAHIDGSVEGRSASFAAGDEKGALAMEEGDVSAKWTL
                     KRAGSEGLLPPSGSVVRKDFGAMGFRLLAEEKLFVLDDAETEKDVLKHLGKPEKKSKA
                     ELWGADGLIHQQWFYKKKGIAVDFVKEDKSGPGETYSVSISAPSAKKTSRGIGIGSAQ
                     QEVLKAYAAEINREESDLPETVVVGSIYGGVVFTMKNGKVVSIFIGAAAE"
BASE COUNT          318 a          326 c          459 g          281 t
ORIGIN      
        1 gaccggctgg cgaaagagac cgggcggacg aaagcgtttt acatacggaa gatgatagaa
       61 gacaggatcg acgacatgga agactattat cttgccgcgg aacgacttgc gcaaagccgg
      121 aaattcggct cccgcagcct gacatctgaa gaagcgaggc gtgaacttgg cttggaggat
      181 tgaatacgat ccttcggcac ttggagacct tcggcatatc gaccgtcgga tggcgcagcg
      241 tatcgtcgat tatatggaca gaagagtcgc catgatggac aatcccagga ctttggggaa
      301 agcgctcaag ggaggatttg gcgatttctg gcgctatcgt gtaggagatt accgtgtaat
      361 ttgttcgatt gaggacaaaa acgtgatggt tttggtagtc cgtgtaggtc accgcagtga
      421 tgtgtacaga taaggtgaag catatatgga tgtatttcgg gacgatgcaa cgagtttttt
      481 tgggaggggt atgctatgag gcgttgcttg tattctaccg ttttctctct gttcctgctc
      541 ttctccgcgc cggcggcggc ggttgatttt acggggatat ggaccttttc aaaggaggac
      601 gggacggagt actcgttcga cctggtccag cgcgatacgg cgcttgaagg cacgttttcg
      661 acctacgatc cgttgctgga caagtacggg gaggcgcata tcgacggctc cgtggagggg
      721 cgcagcgcat cgttcgcggc cggggacgaa aagggcgcgc tcgcgatgga ggagggggac
      781 gtctccgcga aatggacgct caaacgcgcc ggaagcgagg ggctcttgcc cccgtcgggc
      841 agcgtggtgc gcaaggattt cggcgcgatg gggttcaggc tgctcgccga ggaaaaactc
      901 ttcgtcctcg acgatgcgga gacggaaaag gacgttctca agcacctcgg caagccggag
      961 aagaagtcga aggccgagct gtggggcgcg gacgggctca tccaccagca gtggttctac
     1021 aagaagaagg gaatcgccgt cgatttcgtc aaggaggaca agtccggccc cggcgagacg
     1081 tattcagttt cgatctccgc gccgtcggca aagaagacga gccggggaat aggaataggg
     1141 agcgcgcagc aagaggtgct gaaggcctat gcggcggaga tcaaccgcga agagagcgat
     1201 ctccccgaaa cggtcgtcgt cggttccatc tacggaggcg tcgtcttcac gatgaagaac
     1261 ggcaaagtgg tctccatctt catcggcgca gcggcggagt agcaatcagc tgatagaaat
     1321 gttcaaattc aatgcagcct aaggcgtccg gaacggttac gacttaggct gcatcaggtt
     1381 ctgc
//
LOCUS       DAOTIL010000842         1383 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminobacteriaceae bacterium isolate AMR_MDS_3026
            NODE_113057_length_1383_cov_2.773343, whole genome shotgun
            sequence.
ACCESSION   DAOTIL010000842 DAOTIL010000000
VERSION     DAOTIL010000842.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868050
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminobacteriaceae bacterium (wastewater metagenome)
  ORGANISM  Aminobacteriaceae bacterium
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae.
REFERENCE   1  (bases 1 to 1383)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1383)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:19:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,224
            CDSs (total)                      :: 3,198
            Genes (coding)                    :: 3,184
            CDSs (with protein)               :: 3,184
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1383
                     /organism="Aminobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_113057_length_1383_cov_2.773343"
                     /isolate="AMR_MDS_3026"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:3034609"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1138
                     /locus_tag="P5201_13790"
     CDS             <1..1138
                     /locus_tag="P5201_13790"
                     /inference="COORDINATES: protein motif:HMM:NF014069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="HD domain-containing phosphohydrolase"
                     /protein_id="HRV99665.1"
                     /translation="LLPSVLDLDSLRSTRPDAIELLQKHGVDKIPVKKHEPVTIEEFQ
                     RLISSFTPPIARLNPLLSRVIAHQMGAIYTNIGNRELREEGVRSLFSVGATLASEVRR
                     IPQITLSLGEDAEGKLDAAFHGVNVALVAGHIATRLFPLWPDFIRSVTISGLFHDIGK
                     AFILSSGYSADILKMLRDDRITQTHPLLGEALLRDAGIRSQDILSFVRSHHEVWDGSG
                     YPDGLTGENIPVGARILAVANVFHNVLSEKGEGKKRSDQAISSLIASALGRFDHFIVR
                     ALLSSVGLFPPGSVVELSDGRNGIVLETRERNLIRPRILLMTGLRKQRELAPEIVDLR
                     ITTSVSIRRAIDDFGAMPVPPLAAVGEDRTNVLFRKYRSPARLE"
     gene            1236..1322
                     /locus_tag="P5201_13795"
     tRNA            1236..1322
                     /locus_tag="P5201_13795"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1270..1272,aa:Leu,seq:taa)
BASE COUNT          299 a          382 c          345 g          357 t
ORIGIN      
        1 tttgctcccc tcggttcttg acctcgattc tctgcgttcc acgcgtccgg acgcaatcga
       61 attgcttcaa aaacatgggg tcgacaaaat ccctgtcaag aaacatgagc ccgttacgat
      121 tgaggagttc cagcgtctta tttcttcgtt cactcctccg attgcccggc tgaatccctt
      181 gctttccagg gtcatcgcac atcaaatggg cgccatttat accaacatcg gcaatagaga
      241 actccgtgaa gaaggcgtac gttcactttt ttccgtagga gctactctcg cttcagaagt
      301 ccggcgtatt ccgcaaatta cactctcatt gggcgaggac gccgaaggaa agctcgacgc
      361 cgcgtttcac ggcgtaaacg tggccctcgt tgctggacac atagcaacgc gcctttttcc
      421 tttatggccg gattttatcc gctcggtaac tattagcggg ctttttcatg atattggaaa
      481 ggcgtttatt ctctcgtcgg gctactccgc ggatatatta aagatgctcc gtgacgacag
      541 aatcacccaa acgcacccgc ttcttgggga agctcttctt cgggatgccg gaatccgaag
      601 ccaggacatt ctttcttttg ttcgttctca tcatgaagtc tgggatggca gcggctatcc
      661 cgacggactc acaggagaga atattccggt cggggccaga atactcgcgg tcgccaatgt
      721 ctttcacaac gtactctccg agaagggaga agggaagaaa cgaagcgatc aggcaatctc
      781 ctcgctcatt gcctcagcac tcggcaggtt cgatcacttt atcgtccgcg ccctcctctc
      841 ttcggtcggg ctttttcctc cggggtctgt ggttgaactt tcggatggcc ggaacggcat
      901 agtactcgaa actcgcgagc gcaatctcat ccgtcccaga atcctcctga tgacaggcct
      961 caggaaacaa agagagcttg ctcctgaaat cgtcgatctc cgtatcacca cctccgtttc
     1021 tatacggcgt gcaatagatg atttcggcgc aatgcctgtt cctccgcttg ctgcagtagg
     1081 tgaagacagg acgaatgtgc tcttcaggaa atatcgatca cctgccagat tagagtaaat
     1141 attattaatt tatgtttttc ctttttccta tctgatcctc gcgccgtcta ttgacagtgg
     1201 ccgacgcttg gtgtagaatc gctcctgtcg cttgcgggag tgtggcggaa ccggtagacg
     1261 cagcggactt aaaatcctcc gggcagtggc ccttacgggt tcgagtcccg tcactcccac
     1321 caagaatagg ttatcgcggg gtggagcagt caggaagctc gtcgggctca taacccgaag
     1381 gtc
//
LOCUS       DAOTIL010000843         1381 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminobacteriaceae bacterium isolate AMR_MDS_3026
            NODE_113344_length_1381_cov_1.966817, whole genome shotgun
            sequence.
ACCESSION   DAOTIL010000843 DAOTIL010000000
VERSION     DAOTIL010000843.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868050
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminobacteriaceae bacterium (wastewater metagenome)
  ORGANISM  Aminobacteriaceae bacterium
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae.
REFERENCE   1  (bases 1 to 1381)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1381)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:19:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,224
            CDSs (total)                      :: 3,198
            Genes (coding)                    :: 3,184
            CDSs (with protein)               :: 3,184
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1381
                     /organism="Aminobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_113344_length_1381_cov_1.966817"
                     /isolate="AMR_MDS_3026"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:3034609"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(10..789)
                     /locus_tag="P5201_13800"
     CDS             complement(10..789)
                     /locus_tag="P5201_13800"
                     /inference="COORDINATES: protein motif:HMM:NF024206.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ankyrin repeat domain-containing protein"
                     /protein_id="HRV99666.1"
                     /translation="MALKKVWIHSLFAAVSILVFVSSGARAHDGDAELFRRGSVDDIR
                     AAIEAGADVNARDEEGIPVLTWCAQDNTAQAVALLLAAGADVNAESSRGWTPLMAAAS
                     GNPDPGVVSLLVKAGAEVNAKGEEDWDALMYATWVDAHPSVIGALLKAGADVEASTVH
                     GLTALMLAAENSSTGESVALLLQAGAEANAADGQGLTPLMYAAKSNPLVNVVELLLEA
                     GADPHLCDLKGNTSFSLAAQHNPNPEVALFLLRAGPAPVAP"
     gene            complement(853..>1381)
                     /locus_tag="P5201_13805"
     CDS             complement(853..>1381)
                     /locus_tag="P5201_13805"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRV99667.1"
                     /translation="IARREEELSAIAPKIAAEARVLAQSGETDAARRLLTEESAALAG
                     ASAVRKAEFLSAYGPRVLSPERAEIVKGDISGELTVFFQSQSAPVEDSFLMGQGGTPP
                     QRWAAPLRGSLHFADGAWSLRFRVRELTEAAVPCLADFWLAGRDEKGGVFAGRCLLKT
                     LPPSEHQLFQGRCSL"
BASE COUNT          241 a          491 c          425 g          224 t
ORIGIN      
        1 gcgcctacgt caaggagcga ccggggcggg accggccctg agcagaaaca gcgctacttc
       61 gggattgggg ttgtgttgcg ccgccaggga gaaggacgtg tttcccttga ggtcgcaaag
      121 gtgcggatcg gcgcccgctt cgaggagcag ttcgacgacg ttgacgagcg gattgctctt
      181 cgcggcgtac atcagcggcg tcaacccttg tccgtccgcg gcgttcgcct ctgcgccggc
      241 ctggagcagc agcgcgacgc tctccccggt cgagctgttt tcggcggcaa gcatcagcgc
      301 ggtcaggccg tgaacggtcg aagcctctac gtccgcgccc gccttgagca gcgccccgat
      361 caccgaaggg tgcgcgtcga cccatgttgc gtacatcagc gcatcccagt cctcctcgcc
      421 cttcgcgttc acctccgctc ccgccttcac gagcaaagag acgaccccgg ggtccggatt
      481 cccgcttgcg gcagccatga gcggagtcca tccgcggctt gactccgcat tgacgtcagc
      541 cccagccgcg agcagcagcg cgacggcctg cgcggtattg tcctgagcgc accatgtcaa
      601 taccggaatt ccctcctcgt cgcgcgcatt cacgtccgcc cccgcctcga tggccgcgcg
      661 gatgtcgtcg acgctgccgc ggcggaacag ctcggcatcc ccgtcgtgcg cacgcgcccc
      721 ggaagagaca aaaacgagga tggaaaccgc cgcgaagaga gagtgaatcc acaccttctt
      781 gagagccatt ccatcacgct ccttgaaaaa aagcggggct gtacggacat ccgcataccc
      841 cgctccggtt gtttacaggc tgcaccgtcc ctggaagagc tgatgctccg acggaggaag
      901 cgttttcaga agacagcggc ccgcgaagac gccgcccttc tcgtcccgcc cggcgagcca
      961 gaagtccgcc aggcacggga ccgccgcctc ggtgagctct cgcacgcgga agcggagcga
     1021 ccacgcgccg tcggcaaagt ggaggcttcc cctcagggga gcggcccacc gctgcggggg
     1081 cgtccccccc tgccccatca ggaaggagtc ttcgaccggg gcagactgcg attgaaagaa
     1141 gacggtcagc tcacctgaga tatccccctt gacgatctcc gcgcgctccg gactgagtac
     1201 gcgaggcccg tacgcggaaa gaaactccgc ttttcgcacg gcgctcgccc cggccaaagc
     1261 agcgctttcc tccgtcaaca gacgccgggc ggcatccgtt tcgccgcttt gggccaacac
     1321 cctcgcctcc gcggcgatct tcggagcgat cgccgaaagc tcctcttcgc gtctcgcgat
     1381 c
//
LOCUS       DAOTIL010000844         1380 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminobacteriaceae bacterium isolate AMR_MDS_3026
            NODE_113388_length_1380_cov_3.486792, whole genome shotgun
            sequence.
ACCESSION   DAOTIL010000844 DAOTIL010000000
VERSION     DAOTIL010000844.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868050
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminobacteriaceae bacterium (wastewater metagenome)
  ORGANISM  Aminobacteriaceae bacterium
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae.
REFERENCE   1  (bases 1 to 1380)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1380)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:19:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,224
            CDSs (total)                      :: 3,198
            Genes (coding)                    :: 3,184
            CDSs (with protein)               :: 3,184
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1380
                     /organism="Aminobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_113388_length_1380_cov_3.486792"
                     /isolate="AMR_MDS_3026"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:3034609"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..183)
                     /locus_tag="P5201_13810"
     CDS             complement(<1..183)
                     /locus_tag="P5201_13810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004091582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine carbamoyltransferase"
                     /protein_id="HRV99668.1"
                     /translation="MQTLFRGKHFITLQEWTKEEVDTLLDVSYDLKKDFAMDRPTNYL
                     PNKTVFLMFFEQSTRTR"
     gene            complement(232..>1380)
                     /locus_tag="P5201_13815"
     CDS             complement(232..>1380)
                     /locus_tag="P5201_13815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014163858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YgeY family selenium metabolism-linked
                     hydrolase"
                     /protein_id="HRV99669.1"
                     /translation="FLRDMIALPSESCGEKEVIHRIKAEMEACGYDRVEIDPMGNVLG
                     FIGNGKHLIALDAHIDTVGIGDRTLWTYDPYEGYEDDEIIVGRGASDQEGGMASMVYA
                     GKIIKDLGLPGDFTLMVVGSVQEEDCDGLCWQYIINEDKYTPEFVVLTEPTSCNIYRG
                     HRGRMEIKVSTRGLSCHGSAPERGDNAIYKMAPILQELRALHENLHFDPFLGKGSLTV
                     SEVFFSSPSRCAVADGCTISVDRRLTHGETWEMALQQIRNLPAVKAAGAEVSMYSYDR
                     PSWTGLVYPTECFFPTWVLEENHAACRSMVDGYRDLFKSEPLVDKWTFSTNGVSIMGR
                     YGIPCVGFGPGHEDQAHAPDERTWKSELVKAAALYSVVPSIYTGKYVG"
BASE COUNT          249 a          433 c          406 g          292 t
ORIGIN      
        1 gcgggtgcgg gtggactgct cgaagaacat caggaagacc gtcttgttgg gaagatagtt
       61 cgtcggtctg tccatcgcaa agtccttctt cagatcatag gagacgtcga gcagcgtgtc
      121 gacctcttct tttgtccact cctgaagcgt gatgaaatgc ttgcccctga aaagcgtctg
      181 cattgttttt tttcctcctt gagttgtact ctaaacgagt cgttcggctc tctagccgac
      241 gtactttcct gtgtaaatcg acggcacgac ggagtagagc gcggcggcct tcaccagctc
      301 gctcttccat gtccgctcgt cgggggcgtg cgcctgatcc tcgtgccccg gtccgaaacc
      361 gacgcagggg atgccgtagc gccccataat ggagacgccg ttcgtggaga aagtccactt
      421 gtcgacgagc ggttcgctct tgaaaagatc cctgtacccg tccaccatgg acctgcacgc
      481 cgcgtgattc tcctcgagca cccacgtcgg gaagaagcat tccgtggggt acaccagtcc
      541 cgtccaggaa ggacggtcgt aggagtacat ggaaacctcc gcaccggcgg ccttcaccgc
      601 cggaagattg cgtatctgct gcagcgccat ctcccacgtc tcgccgtgcg tgagtctgcg
      661 gtccacggag atggtacagc cgtccgccac cgcgcagcgc gacggagagc tgaagaagac
      721 ctccgacacg gtaaggcttc ccttgccgag gaacgggtcg aagtgcaggt tctcgtgaag
      781 ggcgcgaagc tcctgaagaa tcggcgccat cttgtagatc gcgttgtcgc cccgttccgg
      841 agcggacccg tggcaggaga gccccctcgt gctcaccttg atctccatac ggccgcggtg
      901 gccgcggtag atgttgcacg acgtgggctc ggtaagcacc acaaactccg gcgtgtactt
      961 gtcctcgttg atgatgtact gccagcagag gccgtcgcaa tcctcctcct ggacggagcc
     1021 caccaccatc agcgtgaagt cgcccggaag ccccagatcc ttgatgatct tccccgcgta
     1081 gaccatggag gccatgccgc cctcctggtc gctggcgcct ctgccgacga tgatctcgtc
     1141 gtcctcgtac ccctcgtagg ggtcgtaggt ccaaagggtg cggtcgccga ttccgacggt
     1201 atcgatgtgc gcgtcgaggg cgatcaagtg tttgccgttc ccgatgaagc cgagcacgtt
     1261 ccccatgggg tcgatctcga ctctgtcgta cccgcaggcc tccatctcgg ccttgatgcg
     1321 atggatcact tccttctccc cgcagctctc gctgggaaga gcgatcatgt cccgaaggaa
//
LOCUS       DAOTIL010000845         1378 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminobacteriaceae bacterium isolate AMR_MDS_3026
            NODE_113637_length_1378_cov_3.228269, whole genome shotgun
            sequence.
ACCESSION   DAOTIL010000845 DAOTIL010000000
VERSION     DAOTIL010000845.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868050
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminobacteriaceae bacterium (wastewater metagenome)
  ORGANISM  Aminobacteriaceae bacterium
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae.
REFERENCE   1  (bases 1 to 1378)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1378)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:19:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,224
            CDSs (total)                      :: 3,198
            Genes (coding)                    :: 3,184
            CDSs (with protein)               :: 3,184
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1378
                     /organism="Aminobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_113637_length_1378_cov_3.228269"
                     /isolate="AMR_MDS_3026"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:3034609"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..821
                     /locus_tag="P5201_13820"
     CDS             <1..821
                     /locus_tag="P5201_13820"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HRV99670.1"
                     /translation="AGHSENILALGGAGRLVGIAEGDDETLFPGVPRLPMRADAERIL
                     ALAPDLVLVRPLAESVNEGVFRTLERSGVPVVSLSPPIPETMEAYLERLSSLLGAEGG
                     VNLWKEALALLEAQVLPLDVARPLVFLETSAKGLRTCAPGSWAALLLSLAGGVNAAAS
                     AVPLRPESPLANWGEERLLALAAEGLDVYIVQTGAMNPVTEADVLARPWIKGVLGAEI
                     VLLPEEDVSRPSLLRMKTSVEMLRAIFERGRRENHELPGAKEPMLSGDEEEDER"
     gene            818..>1378
                     /locus_tag="P5201_13825"
     CDS             818..>1378
                     /locus_tag="P5201_13825"
                     /inference="COORDINATES: protein motif:HMM:NF012799.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="precorrin-2 C(20)-methyltransferase"
                     /protein_id="HRV99671.1"
                     /translation="MKFWGVGLGPGDPELVTLRALRILKEADAVFIPFSEKGRESVAG
                     RILEAHLVRETLPVHFPMIRDDETRDALLCEELARLRPFWENAASVALPVIGDSALYA
                     TAAYLFDALKSMAPDVELGLVPGVSAHSLASSRAGRFLALGSEPLSVIPGTAPLARIE
                     AMLAAADSAVLYKPSALGEELRSLVER"
BASE COUNT          212 a          419 c          481 g          266 t
ORIGIN      
        1 acgccggaca cagcgagaac attctcgccc tcgggggggc cggcaggctt gtcggaatcg
       61 cggagggcga cgacgaaacg ctctttcccg gcgtcccccg gctgccgatg cgggccgacg
      121 cggagcgaat tcttgcgctt gcgccggatc ttgtgctcgt ccgccctctt gccgagagcg
      181 tgaatgaagg cgttttcagg acgctggagc gaagcggcgt ccccgtggtt tcgctctctc
      241 cgccgattcc tgagacgatg gaggcgtacc ttgagcgcct ctcctcgttg ctcggggcgg
      301 agggcggagt gaatctctgg aaagaggcgc tcgctcttct ggaggcgcag gttctccccc
      361 tagatgtcgc ccgtccgctc gtcttcctgg agacttcggc aaagggcctc cgcacgtgtg
      421 cgcccggctc atgggcggct ctgctgctct cgctcgccgg aggagtgaac gccgccgcgt
      481 ccgcggttcc gctgcgtccc gagagccccc ttgcgaactg gggggaggaa cgcctccttg
      541 cgcttgctgc ggaggggctc gacgtctaca tcgttcagac gggggcgatg aatccggtca
      601 cggaagcgga cgtcctcgct cgcccgtgga tcaaaggcgt gctcggagcg gagatcgtcc
      661 tgcttcccga ggaggacgtc agccgtccgt cgctgctgcg tatgaaaacg tcggtggaga
      721 tgttgcgtgc gatttttgaa cgaggacgga gagaaaatca tgaactgccc ggagcaaaag
      781 agcctatgct ctccggggat gaggaggaag acgaacggtg aaattctggg gagtgggcct
      841 cggcccgggc gatcccgagc tggtaacgct ccgggcgctg cgtattctga aggaggcgga
      901 cgccgtcttc attccgtttt cggagaaggg aagggagagc gtggcgggac ggatactgga
      961 agcgcatctt gttcgcgaga cgctgcccgt ccatttcccg atgatccgcg acgacgaaac
     1021 gcgcgatgcg ctgctgtgcg aggagcttgc gcggctccgg ccgttctggg agaacgcggc
     1081 ctccgtggcg ctgcccgtca tcggcgattc cgccctctac gcaacggcgg catatctttt
     1141 cgacgcgctg aagagcatgg cgccggatgt cgaactcggc cttgttccgg gcgtttcggc
     1201 tcattcgctg gcctcttcgc gtgcagggcg ctttttggct ctgggaagcg aaccgctctc
     1261 ggtcattccg ggaaccgcgc cgctcgcgcg gatagaagcg atgctcgccg ctgcggactc
     1321 ggcggtcctc tacaagccgt ccgcgctcgg agaggagctg cgttcactgg tggagcgc
//
LOCUS       DAOTIL010000846         1374 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminobacteriaceae bacterium isolate AMR_MDS_3026
            NODE_114083_length_1374_cov_4.734647, whole genome shotgun
            sequence.
ACCESSION   DAOTIL010000846 DAOTIL010000000
VERSION     DAOTIL010000846.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868050
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminobacteriaceae bacterium (wastewater metagenome)
  ORGANISM  Aminobacteriaceae bacterium
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae.
REFERENCE   1  (bases 1 to 1374)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1374)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:19:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,224
            CDSs (total)                      :: 3,198
            Genes (coding)                    :: 3,184
            CDSs (with protein)               :: 3,184
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1374
                     /organism="Aminobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_114083_length_1374_cov_4.734647"
                     /isolate="AMR_MDS_3026"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:3034609"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..382)
                     /locus_tag="P5201_13830"
     CDS             complement(<1..382)
                     /locus_tag="P5201_13830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008522628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HRV99672.1"
                     /translation="MEGREISLVLGTAGHIDHGKTTLVKAVTGVDCDRLIEEKKRGIT
                     IELGFAPLQLDDGRIVSIVDVPGHERFIRQMVAGASGIDGVLLVIAADEGVMPQTREH
                     LEILSLLGVKDGFVALTKTDAVDED"
     gene            complement(418..>1374)
                     /gene="selA"
                     /locus_tag="P5201_13835"
     CDS             complement(418..>1374)
                     /gene="selA"
                     /locus_tag="P5201_13835"
                     /EC_number="2.9.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005662385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004125 - L-seryl-tRNASec selenium
                     transferase activity [Evidence IEA];
                     GO_process: GO:0016260 - selenocysteine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-seryl-tRNA(Sec) selenium transferase"
                     /protein_id="HRV99673.1"
                     /translation="NAGAVLLSLAALCQGKEVIVSRGELVEIGGSFRIPDIMAFSGVR
                     LVEVGATNRTHLRDYANAVTENTAALLKVHPSNFRMEGFVSSTPREELASLAKDRGLF
                     FIEDLGSGMLIDYSRCGLLGEPTVRDCIEQGVHLVTFSGDKMLGGPQIGGVAGKKQLV
                     DALRTYPLHRALRVDKMTLAAFEATLRLYLRGEEKRIPTVSMLFAESHSLQLRAEALL
                     NTLRSSFPNGQFTLTEVDDAVGGGAFPAEKLPGWGVSVSMEDWGSAGRVQRLLRGAQP
                     PVVAGARENETILHVRTLLPGDEERILEAFRFAETRALRGNE"
BASE COUNT          291 a          427 c          341 g          315 t
ORIGIN      
        1 aatcctcgtc gacagcgtca gttttcgtga gcgccacaaa gccgtccttt acgccgagaa
       61 ggctgagtat ttccaagtgc tctctcgtct gcggcatgac gccctcgtct gcggcgatca
      121 cgagaagaac gccgtcgatt ccggaagctc ccgcaaccat ctggcggatg aaccgttcgt
      181 gccccggtac gtccacaatg cttacaatac ggccgtcatc cagttgcaga ggagcaaaac
      241 caagttcaat cgtgatgccg cgcttttttt cttctatcaa tcggtcgcag tcaacgcccg
      301 tcacggcctt tacgagcgtt gtcttcccgt ggtctatatg accggcagta cccagcacca
      361 gagagatttc tctgccttcc acattcttca tcctctctcc atcttcgttg cgctctccta
      421 ttcgttgccc cgaagagctc ttgtttctgc gaaacgaaac gcttcgagaa tccgttcttc
      481 gtcccctgga aggagtgtac gcacatgaag tatagtttcg ttctcacgtg ctcctgcaac
      541 taccggaggt tgtgctcccc taagaagacg ctgtaccctt cctgcactcc cccagtcttc
      601 catggaaacg gaaacgcccc agccgggaag cttctccgcc ggaaatgcgc ctccgcctac
      661 cgcatcgtcc acctccgtca gcgtaaactg cccgttcggg aaggacgacc gaagggtgtt
      721 gagcagagct tccgccctca actgtaagga atgcgattcg gcgaagagca ttgataccgt
      781 ggggattcgt ttttcttctc cccgcagata gagccgcaga gtggcctcga atgcagcaag
      841 ggtcattttg tccacccgga gcgcgcgatg cagcggatag gttctcaggg cgtcaacgag
      901 ctgtttcttt cccgccactc ctccgatctg cgggccgcca agcatcttgt cgcctgagaa
      961 ggttacaagg tgcactccct gttcaataca atctctcact gtaggttccc ccaacagccc
     1021 gcatcgggaa taatcgatca acatcccgct gccgaggtcc tcgatgaaga aaagtccccg
     1081 atcctttgcg agagaagcca gttcttcgcg cggcgtagaa gaaacaaaac cctccatgcg
     1141 gaaatttgaa ggatgtacct tgagcagcgc cgccgtattc tccgttacag cgttcgcgta
     1201 gtcccgcaga tgcgttctgt tggttgcgcc gacctctaca aggcgaacgc cggaaaatgc
     1261 catgatatcc gggatacgga atgatccgcc tatctccacg agttctcccc gggagacaat
     1321 cacttctttc ccctgacaga gcgctgcaag agagagaagg acggctcctg cgtt
//
LOCUS       DAOTIL010000847         1369 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminobacteriaceae bacterium isolate AMR_MDS_3026
            NODE_114726_length_1369_cov_3.330289, whole genome shotgun
            sequence.
ACCESSION   DAOTIL010000847 DAOTIL010000000
VERSION     DAOTIL010000847.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868050
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminobacteriaceae bacterium (wastewater metagenome)
  ORGANISM  Aminobacteriaceae bacterium
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae.
REFERENCE   1  (bases 1 to 1369)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1369)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:19:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,224
            CDSs (total)                      :: 3,198
            Genes (coding)                    :: 3,184
            CDSs (with protein)               :: 3,184
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1369
                     /organism="Aminobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_114726_length_1369_cov_3.330289"
                     /isolate="AMR_MDS_3026"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:3034609"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1176)
                     /locus_tag="P5201_13840"
     CDS             complement(<1..1176)
                     /locus_tag="P5201_13840"
                     /inference="COORDINATES: protein motif:HMM:NF012242.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0140662 - ATP-dependent protein folding
                     chaperone [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp70 family protein"
                     /protein_id="HRV99674.1"
                     /translation="MSAPVLGIDLGTRYALSAVPVKGKGVLVVPSRWGGGRTPSVTAL
                     TQSGWLAGEDAARSEMKNPSTTWWDMKRKLGSPWRARCGRASYTAEDVLVPLLTLLRE
                     DAEAFLGTFISSCVLTVPAFFSFAERSAMGRTARSSGFSSVRIVNEPTAAALAFGIMG
                     KFLILDYGAGTVDISVVESEGGVWQVLESAGLPASGGRDFDILLASSLNEKMKLPRLE
                     DNSALWRALLSEAESLKIALSSCASCEWTPPAGVSCPSLRVTREEFEDLIRPSIEQVT
                     TAVRRLWMRFKPSKLLLVGGGSRIPLLRALLEERVARPEHLNQCPDESVVTGAALYGI
                     SPTKERLLIDVLSQDLGILAADGTPVTLLEKGLHLPVRAERHFYSVGNGPFTMSVFQG
                     "
     gene            complement(1173..>1369)
                     /locus_tag="P5201_13845"
     CDS             complement(1173..>1369)
                     /locus_tag="P5201_13845"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRV99675.1"
                     /translation="LLESVSSPESSDGAGWVFSGLSPSFLRVTGGGESGADPEFSAGD
                     EVLVRITGSRDDSLKGRLIQ"
BASE COUNT          334 a          417 c          369 g          249 t
ORIGIN      
        1 gccctggaaa acgctcatag tgaacgggcc attgccgaca ctgtagaaat gcctttccgc
       61 acgaacagga agatgcaagc ctttttccag gagcgtcacc ggagtaccgt cggcagccaa
      121 tattcccaag tcctgcgaga ggacgtcgat gaggagacgc tcctttgtag gagatatgcc
      181 gtagagagca gcgcccgtaa cgacggactc gtcggggcat tgattgagat gttcaggcct
      241 ggcgacacgc tcttccagca gggcgcgaag aagcggtatc ctgcttcctc cacccaccag
      301 caggagcttt gaaggcttga acctcatcca gagacggcgc accgccgtcg tcacctgctc
      361 gatggatgga cgaatcagat cttcgaactc ctcgcgcgtt acgcgaagag aagggcagga
      421 aactcccgcc ggaggcgtcc attcacacga agcgcaggag gaaagagcaa ttttaagaga
      481 ttccgcttca gaaagaagcg ctctccacaa ggcggagttg tcttcaagac gcggcagctt
      541 catcttctcg ttcaaactgg atgcaagaag aatgtcaaaa tcccgccctc ccgaggcagg
      601 caaaccggcg ctctcaagca cctgccatac gccgccttcg ctctccacaa cagaaatgtc
      661 cacagtcccc gcgccgtaat cgaggatgag aaatttcccc attatgccga atgcaagcgc
      721 agctgccgtc ggttcgttta ctatgcgaac ggatgaaaag cccgaggaac gcgccgttct
      781 tcccatggca gatcgctccg cgaagctgaa aaaagcggga acggtaagaa cgcaggagga
      841 gataaaggtt ccgaggaacg cctcggcgtc ctcccgcaac aacgtcagca gaggaacaag
      901 cacgtcttcc gccgtgtagg aagcgcgacc gcatcgtgcc cgccatggag aaccaagttt
      961 tcgtttcatg tcccaccagg ttgtggacgg gtttttcatt tcacttcgcg ccgcatcctc
     1021 cccggcgagc caccccgatt gcgtcaaggc cgttaccgaa ggcgttcgtc ctcctcccca
     1081 gcggctcggt accaccagga ctccctttcc cttaacaggg actgcggaga gcgcatatcg
     1141 cgttccaaga tcaataccaa ggacaggggc gctcattgaa tcaggcgacc tttcaaagaa
     1201 tcatcacgtg agccggtaat cctgacaagg acctcatctc ctgctgaaaa ttcaggatcg
     1261 gctccggatt cccctcctcc cgtcacgcgg agaaaggacg gagaaagccc gctaaatacc
     1321 catcccgcgc cgtcggagga ttcgggggac gagacgctct ccaagagca
//
LOCUS       DAOTIL010000848         1369 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminobacteriaceae bacterium isolate AMR_MDS_3026
            NODE_114741_length_1369_cov_2.875190, whole genome shotgun
            sequence.
ACCESSION   DAOTIL010000848 DAOTIL010000000
VERSION     DAOTIL010000848.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868050
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminobacteriaceae bacterium (wastewater metagenome)
  ORGANISM  Aminobacteriaceae bacterium
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae.
REFERENCE   1  (bases 1 to 1369)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1369)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:19:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,224
            CDSs (total)                      :: 3,198
            Genes (coding)                    :: 3,184
            CDSs (with protein)               :: 3,184
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1369
                     /organism="Aminobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_114741_length_1369_cov_2.875190"
                     /isolate="AMR_MDS_3026"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:3034609"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..390)
                     /locus_tag="P5201_13850"
     CDS             complement(<1..390)
                     /locus_tag="P5201_13850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014162454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldolase/citrate lyase family protein"
                     /protein_id="HRV99676.1"
                     /translation="MVNELKRKLARGEVALGTFITLNCPDLVEIAGLAGFDFCIIDTE
                     HGPGDAESIQHMIRAAELRGITPVVRVTNPEATTILRILDVGAAGIQAPQVNSRKTAE
                     DVVRFAKYFPKGERGACLTRSSRYGFVE"
     gene            complement(497..>1369)
                     /locus_tag="P5201_13855"
     CDS             complement(497..>1369)
                     /locus_tag="P5201_13855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013048698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isocitrate lyase/PEP mutase family protein"
                     /protein_id="HRV99677.1"
                     /translation="LVVPGVYDALSARICEMVGFEAVFHSGYGTSAVHLGQPDIGLLD
                     MTEMSSQVRSIARAVRIPVLGDSDHGFGNAVNAYRTVQEYLWAGAAGLFMEDQVSPKR
                     CGHMEGKQVISKEEMMGKLRAALDARNELDPDFVVMYRTDAIHVTGFEDALERAKAAV
                     DVGVDMVFVEALETREQMERVVAEVKVPLMLNLIEGGKTPLVPVKEAEAMGFKLIVPA
                     LSTLYSAARGMYDVMKRIHAEGVSDGYLDKLFTFKEFGELVRLDEVRAKEEKYLPPSV
                     MQDKYRGKKHIVEK"
BASE COUNT          238 a          478 c          383 g          270 t
ORIGIN      
        1 ctccacaaac ccgtaccggg acgaccgcgt aagacatgcg cctcgctccc ccttcgggaa
       61 gtacttcgcg aagcgcacca cgtcctcggc cgtcttccgg gagttcacct ggggagcctg
      121 gattccggcg gcgcccacgt cgaggatgcg gagaatcgtg gtggcctccg ggttcgtcac
      181 cctgacgacc ggcgtgatcc cccgaagttc ggcggcgcgg atcatgtgct ggatggactc
      241 cgcgtcgccg gggccgtgct cggtgtcgat gatgcagaag tcgaaccccg cgagccccgc
      301 gatctcgacc agatcggggc agttcagcgt gatgaacgtg ccgagagcga cttcgccccg
      361 ggcaagcttt cttttcagtt cattgaccat tcaagtatcc ctccgtcgac tgcattctgc
      421 gtttccgata catattatac cctcgaaaaa ggggcggagg ttttcccccg cccctgcgtt
      481 tcgacaaact tcccggtcac ttttcgacga tgtgcttctt ccccctgtac ttgtcctgca
      541 tcacgctcgg ggggaggtac ttctcctcct tcgcgcggac ctcgtcgagg cggacaagct
      601 cgccgaactc cttgaacgtg aagagcttgt cgagatagcc gtcggagact ccctcggcgt
      661 gaatcctctt catgacgtcg tacatgcctc gggctgcgga gtagagcgtc gacagcgcgg
      721 gtacgatcag cttgaagccc atggcctcgg cctccttcac cgggacgagc ggcgtcttgc
      781 ccccctcgat gaggttgagc atcagcggaa ccttgacctc cgcgacgacc cgctccatct
      841 gctccctggt ctccaaggcc tcgacaaaga ccatgtccac gccgacatcc accgccgctt
      901 tcgctctttc aagcgcatct tcgaaccccg tcacgtggat ggcgtcagtc cggtacatca
      961 cgacgaagtc gggatccagc tcatttctcg cgtcgagcgc cgcccgcagc ttgcccatca
     1021 tctcctcttt ggagatgacc tgcttcccct ccatatgccc gcaccgcttc ggcgacacct
     1081 gatcctccat gaagaggccg gccgcccccg cccagaggta ctcctgcacc gtccggtacg
     1141 cgttgaccgc gttgccgaac ccgtggtccg agtcgcccaa aacgggaatc cgcacggctc
     1201 tggcgatcga gcgaacctgg gaggacatct ccgtcatgtc gagcagcccg atgtccggct
     1261 gtccgagatg gactgccgac gtgccgtatc ccgagtgaaa gaccgcctcg aagccgacca
     1321 tctcgcagat gcgcgcgctc agcgcgtcgt acacccccgg aacgacaag
//
LOCUS       DAOTIL010000849         1366 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminobacteriaceae bacterium isolate AMR_MDS_3026
            NODE_115081_length_1366_cov_3.356979, whole genome shotgun
            sequence.
ACCESSION   DAOTIL010000849 DAOTIL010000000
VERSION     DAOTIL010000849.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868050
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminobacteriaceae bacterium (wastewater metagenome)
  ORGANISM  Aminobacteriaceae bacterium
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae.
REFERENCE   1  (bases 1 to 1366)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1366)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:19:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,224
            CDSs (total)                      :: 3,198
            Genes (coding)                    :: 3,184
            CDSs (with protein)               :: 3,184
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1366
                     /organism="Aminobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_115081_length_1366_cov_3.356979"
                     /isolate="AMR_MDS_3026"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:3034609"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..624
                     /locus_tag="P5201_13860"
     CDS             <1..624
                     /locus_tag="P5201_13860"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRV99678.1"
                     /translation="EDIRTYLARLFLGKTKVHSLRPQGKEVVLRTTGRVIETHPLRSP
                     ERVLGSALFGSAMDVLTKGFCISEAAIGGIRDNIRGGKFSFDRRLVVNGERVVRDTRV
                     FLHSIGGFTVRCNKAVGRISPLPIEVRNRLPWFRGRSNSLKKGEVLLALYCPILEDGV
                     LKSVLNKERGTLLVWYNPRRAWKARSLFLIRNFSSGGSLEWKWIDIH"
     gene            740..1183
                     /locus_tag="P5201_13865"
     CDS             740..1183
                     /locus_tag="P5201_13865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013048894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S1 RNA-binding domain-containing protein"
                     /protein_id="HRV99679.1"
                     /translation="MADEKKGDVTPLAPGEVVVCVVEQIMPYGAFVRIKNGQRAMIHI
                     SELSHGFVKKVEDILALGQEVQARVIKIDEKGRIDLSLKKMEAPRPAASFAPRSAAAN
                     VPEDPRDSFEKKLSNYLKASEAKIADINSKLNSSKASKKRGKPAK"
BASE COUNT          315 a          327 c          400 g          324 t
ORIGIN      
        1 gaggatattc gcacgtatct ggcaaggctc tttctcggaa aaacgaaagt acactctctc
       61 cgtcctcaag ggaaagaggt tgtgcttcgg actaccggga gagttataga gacgcatcct
      121 ctccgctctc ccgaacgcgt acttggttcc gccctgttcg gttctgcgat ggatgtgctg
      181 actaagggct tttgtatttc agaggcggcg atcggaggca ttcgggacaa tatccgcggc
      241 gggaagttct ctttcgacag gcgcctcgtg gtgaacggcg aaagagttgt gcgcgatacg
      301 cgggtttttt tgcactccat cggtggattc actgtacgtt gtaacaaggc tgtaggcagg
      361 atctcgcccc ttccgattga agtgcggaat cggcttccct ggtttcgagg aaggtcgaat
      421 tctctcaaga agggagaagt tttgcttgcg ctttactgcc cgatactgga agacggcgtg
      481 ttgaaaagtg ttctgaacaa ggagagagga acgcttcttg tttggtataa tcctcgaagg
      541 gcgtggaaag cccggagttt gtttcttatt cggaacttct cctctggagg gagtctcgaa
      601 tggaaatgga tcgatattca ctagcgcata atcgcagcct gcggaggtgg ttttcaccgg
      661 aaacgaaaca gcggcacagc ttgtcgatgc agtaaggact caaagtatca acaaataatc
      721 gtggagggta gacaaccgca tggcagatga aaaaaaggga gacgtcactc ccctggcacc
      781 gggtgaagtg gttgtgtgcg ttgtagagca gattatgccc tacggcgctt ttgttcggat
      841 taaaaacgga cagcgggcga tgatccatat ctccgagctg tcccacgggt tcgtcaagaa
      901 agtcgaggat atcctcgctc ttggccagga ggtccaggcc agggtaataa agatcgacga
      961 gaagggcaga atcgatctct ccctgaaaaa aatggaggcc ccccggccgg ctgcttcctt
     1021 tgctccgcga agcgctgcgg cgaacgttcc tgaagatccc cgggactcct tcgagaagaa
     1081 gctttcgaac tatctcaagg ccagcgaggc gaagatcgcc gatatcaaca gcaaactcaa
     1141 cagcagcaag gcttcaaaga aaagaggcaa acctgcgaag tgaagcggac tctttctttt
     1201 ttctcccggg ggcggattcg cccccttctt tttggggagc cgctgaatgg aagggataga
     1261 tctgttctgc tgcgccagat gaaaggacgt gttttcgacc cttcgctggt ttctgctgtt
     1321 gtacgccgct gtccgaaggg gttccctgcg gtgatacagt gcgttc
//
LOCUS       DAOTIL010000850         1365 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminobacteriaceae bacterium isolate AMR_MDS_3026
            NODE_115202_length_1365_cov_3.489313, whole genome shotgun
            sequence.
ACCESSION   DAOTIL010000850 DAOTIL010000000
VERSION     DAOTIL010000850.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868050
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminobacteriaceae bacterium (wastewater metagenome)
  ORGANISM  Aminobacteriaceae bacterium
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae.
REFERENCE   1  (bases 1 to 1365)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1365)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:19:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,224
            CDSs (total)                      :: 3,198
            Genes (coding)                    :: 3,184
            CDSs (with protein)               :: 3,184
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1365
                     /organism="Aminobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_115202_length_1365_cov_3.489313"
                     /isolate="AMR_MDS_3026"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:3034609"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..237
                     /locus_tag="P5201_13870"
     CDS             <1..237
                     /locus_tag="P5201_13870"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-4-keto-pentose/UDP-xylose
                     synthase"
                     /protein_id="HRV99680.1"
                     /translation="IDVMKEIPSFREAAEKTAVTVMPAEQYYGNGYDDMKNRVPSIQA
                     IGAALGWKPLVPLREAVAMTVASYLDSGEEQKRG"
     gene            230..1150
                     /locus_tag="P5201_13875"
     CDS             230..1150
                     /locus_tag="P5201_13875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008709288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HRV99681.1"
                     /translation="MVDTVLVLKTDVDTLRGYREGVPRLLEIFRARKMRASFFFSFGP
                     DNSGKAIRRIFKKGFLQKMRRTKAASAYGLRTLLYGTLLPAPKIVESEPSVFIRAVEE
                     GQDCGIHSWDHVHWHDNLSKMSEEQIRGEFRKAMELFERYARRTAKSCAAPGWQVTPA
                     SLAVQDELGFDYCSDVRGSGYAFRPRMRGRTFKTLQIPTTLPTLDEVLGDVPGDRIND
                     HYMELLQPGLNVHTIHAEMEGGGQSALFEELLDRCVKRGVVFKTLDDIALEISLKKPI
                     IHTCDVVAGATPGRWGTVSVQRCAREETAD"
BASE COUNT          276 a          431 c          418 g          240 t
ORIGIN      
        1 atcgacgtga tgaaagagat cccctctttc cgggaggccg ctgaaaagac cgccgtgacg
       61 gtgatgcccg ccgaacagta ttacggcaac ggctacgacg acatgaagaa ccgcgttcct
      121 tcgattcagg ccatcggcgc cgcgctcggc tggaagccgc tcgtgccgct ccgcgaggcc
      181 gtggcgatga ctgtggcgag ctaccttgat tcaggagagg agcagaagcg tggttgatac
      241 ggtgctcgtc ctcaagacgg acgtcgatac gctgagaggc taccgcgagg gcgtcccccg
      301 actcctggag atcttccgcg ccaggaagat gcgagcctcg ttcttcttct ccttcggtcc
      361 ggataactcc ggcaaggcga tccggcgtat cttcaaaaaa gggttcctgc agaagatgcg
      421 gcgtaccaag gccgcctcgg cgtacggact ccgcaccctg ctctacggca cgctgcttcc
      481 ggcgccgaaa atagtggaga gcgagccgag cgtcttcatc cgggctgtcg aggagggaca
      541 ggactgcggc attcacagct gggaccacgt ccactggcac gacaaccttt caaaaatgag
      601 cgaggagcag atacgcgggg agtttcgaaa ggcgatggag ctcttcgagc ggtacgcaag
      661 gagaacggcg aaaagctgcg ccgcgcccgg ctggcaggtc acgcccgcga gcctcgccgt
      721 gcaggacgaa ctgggcttcg actactgcag cgacgtccgc gggagcggat acgccttccg
      781 tccgcgcatg cgagggcgta ccttcaagac gctccaaatc cccacaacgc ttcccactct
      841 cgacgaagtg ctcggcgacg ttccggggga caggatcaac gatcattaca tggagcttct
      901 tcagcccggg ctgaacgtgc acaccatcca cgcggagatg gaggggggcg gacagagcgc
      961 gctcttcgag gagctgctgg accgctgcgt gaagagaggc gtcgtcttca agacgctcga
     1021 cgacatcgcc ctggagattt cattgaagaa accgatcatc cacacgtgcg acgtcgtcgc
     1081 cggagcaacc cccggccgct gggggacggt ttccgtccag cggtgcgccc gcgaagaaac
     1141 ggcggactaa aaaagagggg gccagcaagc cccctcttct gtactccgta tttatttcac
     1201 tggaacgagc cctccgctct gaacaccgtc ttcgtcatcc accttgaaac gctccgacat
     1261 ccggcgcaga tcctcggcgg tctcgctgac ctcctgagag cttgtcgcga cgttctccat
     1321 agccgctgcg gtctcctcca tggctgccgc catgctctgc acgtt
//
LOCUS       DAOTIL010000851         1365 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminobacteriaceae bacterium isolate AMR_MDS_3026
            NODE_115254_length_1365_cov_2.245038, whole genome shotgun
            sequence.
ACCESSION   DAOTIL010000851 DAOTIL010000000
VERSION     DAOTIL010000851.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868050
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminobacteriaceae bacterium (wastewater metagenome)
  ORGANISM  Aminobacteriaceae bacterium
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae.
REFERENCE   1  (bases 1 to 1365)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1365)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:19:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,224
            CDSs (total)                      :: 3,198
            Genes (coding)                    :: 3,184
            CDSs (with protein)               :: 3,184
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1365
                     /organism="Aminobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_115254_length_1365_cov_2.245038"
                     /isolate="AMR_MDS_3026"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:3034609"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..366)
                     /locus_tag="P5201_13880"
     CDS             complement(<1..366)
                     /locus_tag="P5201_13880"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome small subunit-dependent GTPase"
                     /protein_id="HRV99682.1"
                     /translation="MNTKILERIGFDRRFADEASMYPGFYAGRVVSQSKDLYRVTTEE
                     SDLTAEISGKFRFEVRTPSDYPAVGDFVLLDRNEDSSGNAIIHRVLTRKSVFIRRAAG
                     TSHEEQVVAANIDTVFICMS"
     gene            787..1224
                     /locus_tag="P5201_13885"
     CDS             787..1224
                     /locus_tag="P5201_13885"
                     /inference="COORDINATES: protein motif:HMM:NF014608.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OsmC family protein"
                     /protein_id="HRV99683.1"
                     /translation="MPGMKEEVNVRLVNQKVQFIGVSSANPEQPLTFDYAPPMGDGAG
                     FNGLELLLMSLCGCSATAVVYLLRKMRKTVSGFEVQAEGIRKETHPIKFEKITLRFIL
                     KSPDAKEEDIQKAVQLTEETYCPVWQMIKNNVDVVPEYTIVRE"
BASE COUNT          325 a          389 c          354 g          297 t
ORIGIN      
        1 cgacatgcag atgaagacgg tgtcgatatt cgccgccacc acctgctcct cgtgcgacgt
       61 ccccgccgcc ctccggatga agacgctctt cctcgtcagc acgcgatgga tgatcgcgtt
      121 gccgctcgaa tcctcgttcc ggtcgagcag cacaaaatcg cccaccgccg gatagtccga
      181 cggcgtgcgc acctcgaaac gaaattttcc cgagatctcc gccgtcaagt cgctctcttc
      241 cgtcgtcacc ctgtacaggt ccttcgactg cgacacgacg cgcccggcgt agaatccggg
      301 gtacatgctt gcttcgtcgg cgaagcgccg gtcgaagccg atacgttcta aaattttagt
      361 gttcactgtt ttccctccaa aaagtatttt tcaatgactt tcgggaggcg gatacatcaa
      421 ctcagcgtgc agtcgcctcc cggactttga caacctccgg cgcgaccctc ttcaacaaca
      481 tacgtcacca ctccttctgt ttccaaataa tttccgccgc ctgatgatag cacgaaagag
      541 cgtgattgca agacgccggg cggcaataaa caaaaaattg aacaaaaaac ttgatttaac
      601 tgataggata tggtaaaagg gcgcagggat aaagttttct tcaagagtgg ctttgatagt
      661 gctctattcc gtgtactgtt tcgccgctgc gcccgaggat accgccttcc cgcgctctgc
      721 gccatctgta cgagaggctg tgctctcgaa cgaatcacat tctaaaagat cgaaggagca
      781 agaaaaatgc ctggaatgaa agaagaagtc aacgtccggt tagtgaacca gaaggtccaa
      841 tttatcggcg tttcatctgc caatcccgaa cagccgctca cgttcgatta cgcgcctccg
      901 atgggcgacg gggcggggtt caacgggctc gagctgctcc ttatgagcct gtgcggctgc
      961 agcgccaccg ccgttgtcta tctgttacga aaaatgcgaa agactgtctc cggattcgaa
     1021 gtacaggcgg aaggaatccg gaaggagacg catccgataa aattcgagaa gattacgctc
     1081 cgcttcatcc tcaaatcgcc ggacgcaaaa gaagaggata ttcagaaggc cgtgcagctt
     1141 accgaagaaa cgtactgccc cgtgtggcag atgataaaga acaatgtcga cgtcgtaccg
     1201 gaatatacaa tcgtcaggga gtagcggcgg cattccggcc gtcctctacg gggagccctc
     1261 cgacaggctg ttccttttcg tccacgggaa gctctcccgg aaagaagagg cggaagcctt
     1321 cgcggaggac gtcggtgatc ttttccttcg tttcttcgcc gtgca
//
LOCUS       DAOTIL010000852         1364 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminobacteriaceae bacterium isolate AMR_MDS_3026
            NODE_115315_length_1364_cov_3.676089, whole genome shotgun
            sequence.
ACCESSION   DAOTIL010000852 DAOTIL010000000
VERSION     DAOTIL010000852.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868050
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminobacteriaceae bacterium (wastewater metagenome)
  ORGANISM  Aminobacteriaceae bacterium
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae.
REFERENCE   1  (bases 1 to 1364)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1364)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:19:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,224
            CDSs (total)                      :: 3,198
            Genes (coding)                    :: 3,184
            CDSs (with protein)               :: 3,184
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1364
                     /organism="Aminobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_115315_length_1364_cov_3.676089"
                     /isolate="AMR_MDS_3026"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:3034609"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..608)
                     /locus_tag="P5201_13890"
     CDS             complement(<1..608)
                     /locus_tag="P5201_13890"
                     /inference="COORDINATES: protein motif:HMM:NF015949.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF364 domain-containing protein"
                     /protein_id="HRV99684.1"
                     /translation="MNTIVEELIEYGLSHSKGERVEDVRIGLGYTAVKLDSAKAGVAC
                     MLRHRLGKTTCSLLPHAGSLAGSPADRIIPLLRSPNIVETSVGLATLNALIQQDRYDE
                     DSSNDDLVQLLNITSNDRVGMVGDITPIFNRIQKHAKECIVFDEGKSAWEGITDTSLE
                     KEMLPRCDVALLSATTLLNNTFESVLAMVAGAREICVIGPSAP"
     gene            936..>1364
                     /locus_tag="P5201_13895"
     CDS             936..>1364
                     /locus_tag="P5201_13895"
                     /inference="COORDINATES: protein motif:HMM:NF013088.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HRV99685.1"
                     /translation="MNAHYVKFISACLLFGSNGIVASHIRMSSHEIVFLRTLIGSLFL
                     LAVFVLTGGKFSGLRNGRHLAFLILSGVAMGASWIFLFEAYQQIGVGMATLAYYCGPV
                     IVMALSPLLFKEKLTPARICGFVAVLAGMFLVNRDALMLDG"
BASE COUNT          284 a          354 c          401 g          325 t
ORIGIN      
        1 ggcgccgaag ggccgatgac gcagatctcc ctcgcgcctg ccaccatcgc gagcacactc
       61 tcgaacgtgt tgttgaggag cgtcgtcgcg gaaagaagag cgacgtcgca gcgtggaagc
      121 atctcctttt caagcgacgt gtcggtaatc ccctcccagg cgcttttccc ttcgtcgaag
      181 acgatgcatt ctttcgcgtg tttttgaatc ctgttgaaaa tgggcgtaat atcgccgacc
      241 attcccaccc ggtcgttcga ggtgatgttg agcagctgga caagatcgtc gttggaagag
      301 tcttcatcgt acctgtcctg ctggataagc gcgttgaggg tcgccagtcc gacggatgtc
      361 tccacgatgt tcggagaacg caggagagga ataatccggt ccgcggggct tccagcaagg
      421 gaaccggcgt gtggcagaag ggagcaggtt gtctttccga ggcgatgtcg gagcatgcac
      481 gcaactccgg cctttgcgga gtcaagcttg accgcggtat agcccagtcc gatccggacg
      541 tcttccacgc gctctccctt ggaatgggag agtccgtact cgataagctc ttcgacgatt
      601 gtattcacag ctatattccc tcctcgacaa gatgaccggg cgtcttaaaa agcgtagaat
      661 ggtgtgcaaa ccggtaaagc ggagaagcag cctttccgcg cattctgacc cgaaaaatgg
      721 agctatgtgt tctgttgaca tgatgccaca aaacagtcaa acatgcaact gataatcatt
      781 atcatattaa agagtgggcc ttgtgccgtg aaccgccgaa gatgcactga agaagttctt
      841 tgatggcgcc actcgataat tcgagcatat gagagagaat agcgctgtat ctaaggagtc
      901 tccgaacttt ttgacttgaa gagaggtgaa gtgtaatgaa cgcacactac gtgaaattca
      961 tatcggcgtg cctgctgttc ggctcgaacg ggatcgtggc cagccatatt cggatgagca
     1021 gtcatgagat cgtctttctg cggacgctga tcggcagtct gtttcttctc gccgtatttg
     1081 tcctgaccgg cgggaagttc tccgggttgc ggaatggacg ccacctcgcg ttcttgattc
     1141 tgtcgggggt cgccatgggc gcgagctgga tttttctctt cgaggcgtac cagcaaatcg
     1201 gcgtcggcat ggcgacgctg gcctactact gcgggccggt tatcgtgatg gcgctctcgc
     1261 cgctgttgtt caaggaaaag ctgactcccg cgcggatatg cggtttcgtc gccgttcttg
     1321 cggggatgtt tctcgtcaac cgcgacgcgc tgatgctgga cggc
//
LOCUS       DAOTIL010000853         1362 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminobacteriaceae bacterium isolate AMR_MDS_3026
            NODE_115574_length_1362_cov_3.771232, whole genome shotgun
            sequence.
ACCESSION   DAOTIL010000853 DAOTIL010000000
VERSION     DAOTIL010000853.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868050
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminobacteriaceae bacterium (wastewater metagenome)
  ORGANISM  Aminobacteriaceae bacterium
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae.
REFERENCE   1  (bases 1 to 1362)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1362)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:19:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,224
            CDSs (total)                      :: 3,198
            Genes (coding)                    :: 3,184
            CDSs (with protein)               :: 3,184
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1362
                     /organism="Aminobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_115574_length_1362_cov_3.771232"
                     /isolate="AMR_MDS_3026"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:3034609"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(238..>1362)
                     /locus_tag="P5201_13900"
     CDS             complement(238..>1362)
                     /locus_tag="P5201_13900"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRV99686.1"
                     /translation="EADEPLPQLPLAAIPEKNEPAVREKPFSSERVAPSRVSAITSSF
                     AHPGGESVESLSSIQLREALADPDISLFAAWGERGELLALQALTASAQRRSLGVAFPP
                     LFTKNAEKEACAATLLNALIEHVEDDGEIEGLLGFVPGKSPAMASLFASKGFAECALL
                     PALPGNSFLKGNVAGMASFRPCALKQIPSRPGAPEDYRDVLRRIDAEEAQKAPLYAPE
                     RLHALLRLLLKEGKQPLLSHPLENQEDFRKRSVISAAAEFQKQRCDLIVHDYGLDFLF
                     QLSGLTSKLLGKDLASLTLHLPMELAATASGAEEAEKAGYVFCGVIPGTPQGSELIYV
                     HCKNLPELLEAAHPATDTGRQLVAWAGEDCRRIRFLTNWD"
BASE COUNT          237 a          391 c          454 g          280 t
ORIGIN      
        1 cacacgatga cgccgtccct ccggagacgc accacgagtt gctgctcgat gcgttccggg
       61 cgacggaagg cgttcaggcg gagggcttca tgctggacga cgatcactcc tactcggcgt
      121 cgcgggtggc gctggcgaag gttcttttgg agtatctgga gaagagaggg tactgacggc
      181 ttttatcgcc gttgtagcgc tctttcggcc ggtctccttg cgccggggct tcgtccgtca
      241 gtcccagttc gtaagaaacc tgattctccg gcagtcttcg cccgcccatg cgacaagctg
      301 tcttcccgta tccgtcgccg ggtgcgccgc ttccaggagt tccggcagat ttttgcagtg
      361 aacgtagatc agttcgctcc cttgcggtgt tccggggatg acgccgcaga agacgtaccc
      421 cgctttttcc gcctcctcgg ctccggaagc ggtggctgca agctccatcg gcagatggag
      481 cgtcagcgag gccaggtctt tcccgagcag tttgctcgtg agtcccgaga gctgaaagag
      541 gaagtccagt ccgtagtcgt ggacgatgag atcgcaccgc tgtttttgga actcggcagc
      601 ggcggagatg acgcttcgct tcctgaaatc ctcctgattt tctagcggat gggaaaggag
      661 cggttgcttt ccctctttca aaagcagccg gagcagcgcg tgaagccgtt ccggcgcgta
      721 cagaggcgct ttctgcgcct cctcggcatc gatccggcga agtacgtcac ggtagtcctc
      781 cggggccccc ggacgggatg gaatttgctt cagggcgcag gggcggaagg atgccatgcc
      841 cgcaacattg cctttaagaa agctgtttcc cggaagcgcg gggagcaggg cgcactcggc
      901 gaaacccttt gaggcgaaca gcgacgccat tgcgggcgac tttcccggaa cgaatccgag
      961 gagcccctcg atctccccat cgtcttcgac gtgttcgatg agcgcgttca acagcgttgc
     1021 cgcacaggct tctttctccg cattttttgt gaagagcggg gggaaggcca cgccgaggga
     1081 gcgccgttgc gcggaggcgg tgagcgcttg cagcgcgagc agctcgcccc gctctcccca
     1141 tgcggcgaag agggagatgt cggggtccgc caatgcttcg cggagttgaa tagaagaaag
     1201 cgattcgacg ctttcccccc cgggatgggc gaaagaggag gtgatggcgg agacgcggga
     1261 gggagcgact cgttcggagg aaaagggttt ttcgcgaacg gcgggctcgt tcttttccgg
     1321 tatcgcagcc agaggaagct gcgggagcgg ttcgtcggct tc
//
LOCUS       DAOTIL010000854         1362 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminobacteriaceae bacterium isolate AMR_MDS_3026
            NODE_115610_length_1362_cov_2.602142, whole genome shotgun
            sequence.
ACCESSION   DAOTIL010000854 DAOTIL010000000
VERSION     DAOTIL010000854.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868050
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminobacteriaceae bacterium (wastewater metagenome)
  ORGANISM  Aminobacteriaceae bacterium
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae.
REFERENCE   1  (bases 1 to 1362)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1362)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:19:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,224
            CDSs (total)                      :: 3,198
            Genes (coding)                    :: 3,184
            CDSs (with protein)               :: 3,184
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1362
                     /organism="Aminobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_115610_length_1362_cov_2.602142"
                     /isolate="AMR_MDS_3026"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:3034609"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..579)
                     /locus_tag="P5201_13905"
     CDS             complement(<1..579)
                     /locus_tag="P5201_13905"
                     /inference="COORDINATES: protein motif:HMM:NF012711.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I adenylate-forming enzyme family protein"
                     /protein_id="HRV99687.1"
                     /translation="MPNSWIGNYSFFRARIDSSREAVYDLDTETRYTYGDLERRANLL
                     ARYLKDQAGVRKGDRVAFIARNCIEMIDAYYATGKLGAIFVPYNARLSVSELVQLVNN
                     ETPKVLFYEEVFADKIPHLKSGCSVEKYLVLSPGKNPENDTTYAEATGHGNDAFVGCD
                     ELDIEDIHMIIHTGGTTGLPKGGLISHRCELFN"
     gene            complement(592..1200)
                     /locus_tag="P5201_13910"
     CDS             complement(592..1200)
                     /locus_tag="P5201_13910"
                     /inference="COORDINATES: protein motif:HMM:NF015325.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin family protein"
                     /protein_id="HRV99688.1"
                     /translation="MKLLALNGSPRADGNTFRYLTMILERAKSLGAETELIQLADKNL
                     RGCRGCYECIAAKRCTLDDDFQGIFPKMAQADAILLGSPVYHSSITAELKAVLDRAGF
                     SGRWAKNEMKKEGESYTWGTTALSGKVVVPVSTARRAGHNFAFAQMLLWAAANDCIIV
                     GNTYWNVGVAGKGGAKNAEEDEEGVGIMTNIADRVVALLKRL"
BASE COUNT          219 a          438 c          415 g          290 t
ORIGIN      
        1 gttgaacagc tcgcagcggt gcgagatcag cccgcccttg gggaggcccg tcgtcccgcc
       61 ggtgtggata atcatgtgga tatcttcgat atcaagctcg tcgcagccga cgaacgcgtc
      121 gttcccgtgc cccgtcgcct cggcgtaggt cgtgtcgttc tccggattct ttccggggga
      181 gaggacgagg tacttctcca cggagcagcc cgacttcaga tgcggtatct tgtccgcgaa
      241 gacctcctcg tagaagagga ccttcggcgt ttcgttgttg acgagctgca ccagttcgct
      301 caccgagagg cgcgcgttgt acggaacgaa gatcgcgccc agcttccccg tggcgtagta
      361 cgcgtcgatc atctcgatgc agttccgcgc gatgaaggcg acgcggtcgc ccttgcgcac
      421 tccggcctga tcttttaagt agcgcgccag caggttcgcc cgccgttcga ggtcgccgta
      481 ggtgtagcgc gtctccgtgt cgaggtcgta gaccgcctcc cgcgaactgt cgatgcgggc
      541 gcggaagaac gagtagttgc ctatccagct gttcggcata ttcagccccc cttacaggcg
      601 cttcagcagg gcgacgaccc tgtccgcgat attcgtcatg atgccgacgc cctcttcgtc
      661 ctcctcggcg ttcttcgcgc cgcccttccc ggcgaccccc acgttccagt aggtgttgcc
      721 gacgatgatg cagtcgttcg ccgcggccca cagcagcatc tgcgcgaagg cgaagttgtg
      781 ccccgccctg cgggcggtgc tcaccggcac gacgaccttt ccggagagcg cggtcgttcc
      841 ccaggtatag ctctcgcctt cctttttcat ctcgttcttc gcccagcggc cggagaagcc
      901 ggccctgtcg agaaccgcct tcagctccgc agtgatcgaa gagtggtaga cgggcgagcc
      961 gagcaggata gcgtcggctt gcgccatttt cgggaagatt ccctggaagt cgtcgtccag
     1021 agtgcagcgc ttcgcggcga tgcactcgta gcaccctctg caccccctga ggttcttgtc
     1081 ggcgagctga atcagctcgg tctccgcgcc gaggctcttc gcacgctcca gaatcatggt
     1141 caggtagcgg aaggtgttgc cgtcggcccg cgggctcccg ttcagggcga gaagtttcat
     1201 ttcgatctcc tccttgggtt tttcaaaaat gccggtccgc ccgcacgagc gaggcggacc
     1261 ggaagaacgc gtctacaggt cgatgacggc ctgcaacttt ttattcagct tctgctcctt
     1321 cgtcatcagc gagacgaaga tcgtcagcga gacggcgatg gc
//
LOCUS       DAOTIL010000855         1362 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminobacteriaceae bacterium isolate AMR_MDS_3026
            NODE_115641_length_1362_cov_2.002295, whole genome shotgun
            sequence.
ACCESSION   DAOTIL010000855 DAOTIL010000000
VERSION     DAOTIL010000855.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868050
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminobacteriaceae bacterium (wastewater metagenome)
  ORGANISM  Aminobacteriaceae bacterium
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae.
REFERENCE   1  (bases 1 to 1362)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1362)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:19:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,224
            CDSs (total)                      :: 3,198
            Genes (coding)                    :: 3,184
            CDSs (with protein)               :: 3,184
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1362
                     /organism="Aminobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_115641_length_1362_cov_2.002295"
                     /isolate="AMR_MDS_3026"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:3034609"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..256
                     /locus_tag="P5201_13915"
     CDS             <1..256
                     /locus_tag="P5201_13915"
                     /inference="COORDINATES: protein motif:HMM:NF013767.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009927 - histidine phosphotransfer kinase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="Hpt domain-containing protein"
                     /protein_id="HRV99689.1"
                     /translation="ESFPEDVELLRQAINNGEFDKAAKVAHTMKGTAGNACARSMSQC
                     AKALQLAAKEEDREKTVRLFEELVAIFDKVREAMLKEMER"
     gene            complement(260..706)
                     /locus_tag="P5201_13920"
     CDS             complement(260..706)
                     /locus_tag="P5201_13920"
                     /inference="COORDINATES: protein motif:HMM:NF017103.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DciA family protein"
                     /protein_id="HRV99690.1"
                     /translation="MGGLLRGVLPRQAEERLALPELRKVWAEIVGGALAAKTYAEDID
                     EGALLVKAESPAAAKMISMRAGSLARELSRRTGLSVTSVKVVPGKASHRPSPPRKEEP
                     FRVVPPKELVERNFEEVKEKFSPERETLARRFASLMALFQKRFPGK"
     gene            complement(723..>1362)
                     /locus_tag="P5201_13925"
     CDS             complement(723..>1362)
                     /locus_tag="P5201_13925"
                     /inference="COORDINATES: protein motif:HMM:NF025300.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis enzyme MnmG"
                     /protein_id="HRV99691.1"
                     /translation="TNEPYRMLTSRCEHRLLLRHDNADRRLAPIGRGLGLLDDERWNR
                     LLSGWKRIDDELERLAHSKIPPTDELNRELAALDSAPISEGTKAVELLRRPGVPYSLV
                     EKYSPSPEPPRGGEALSLEVEVKYEGYIERQGRQVARMERMEHVSLPSDMDYGSVPGL
                     LAESRQKLEAVRPYTLGQAGRISGVTPTDIHLLSVWLNALSRRKERSVAAEE"
BASE COUNT          232 a          451 c          433 g          246 t
ORIGIN      
        1 tgaatccttc ccggaggacg tcgaactgtt gcggcaggcg atcaacaacg gtgagttcga
       61 caaggcggcg aaggtcgccc atacgatgaa gggaacggcg ggaaacgcct gcgcccggag
      121 catgagccag tgcgcgaagg cgctgcagct ggcggcgaag gaagaggacc gggaaaagac
      181 cgtgcggctg ttcgaggagc tggtcgccat cttcgacaag gttcgcgagg cgatgctgaa
      241 ggagatggag cgctgaccgc tactttccgg ggaaacgctt ttgaaagagg gccatcaagg
      301 aggcgaacct gcgtgcaagc gtctcccgct cgggggagaa cttctctttg acctcctcga
      361 aattgcgctc gacaagctcc ttcggcggga caacgcggaa gggctcttcc ttgcggggag
      421 gagacgggcg gtgcgacgcc ttgcccggaa ccaccttgac cgacgtcacg gagagtcccg
      481 tccgcctcga cagctccctc gcgagcgagc cggcccgcat ggagatcatc ttcgccgcgg
      541 cggggctttc cgccttcacc agcagcgcgc cctcgtcgat atcctcggcg tacgtcttcg
      601 ccgcgagcgc gcctccgacg atctccgccc atactttccg gagctccggg agggcgagcc
      661 tctcttccgc ctgtctgggg aggacgcccc gaagcagacc tcccaccttt gaagggtctc
      721 ttctactcct ccgccgccac actgcgttcc ttcctcctcg acagcgcgtt cagccacacc
      781 gaaagcagat gaatatcggt gggcgtcacc ccggagatgc gccccgcctg gccgagcgtg
      841 tacggacgga cggcttccag cttctgcctg ctctcggcga gcagaccggg aacactgccg
      901 tagtccatgt cggaagggag cgagacgtgc tccatccgct ccatgcgcgc cacctgcctc
      961 ccctgccgct cgatataccc ctcgtacttt acctcgactt caagagaaag cgcctccccg
     1021 cctctcggcg gttcagggga gggggagtac ttttcgacga gcgaataggg cactccgggg
     1081 cgccggagca gctccactgc tttcgttccc tccgatatgg gggcggagtc cagcgcggcc
     1141 agctccctgt tgagctcgtc cgtagggggg atcttcgagt gcgcaaggcg ttcaagctcg
     1201 tcgtcgatcc gcttccaccc ggagagcagc ctgttccatc gttcgtcgtc cagcagcccg
     1261 agtccgcgcc ctatgggagc gagcctgcgg tcggcgttgt cgtgccgcag cagcagacgg
     1321 tgttcgcacc tgctggtgag catcctgtac ggctcgttcg tc
//
LOCUS       DAOTIL010000856         1361 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminobacteriaceae bacterium isolate AMR_MDS_3026
            NODE_115688_length_1361_cov_4.502297, whole genome shotgun
            sequence.
ACCESSION   DAOTIL010000856 DAOTIL010000000
VERSION     DAOTIL010000856.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868050
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminobacteriaceae bacterium (wastewater metagenome)
  ORGANISM  Aminobacteriaceae bacterium
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae.
REFERENCE   1  (bases 1 to 1361)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1361)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:19:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,224
            CDSs (total)                      :: 3,198
            Genes (coding)                    :: 3,184
            CDSs (with protein)               :: 3,184
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1361
                     /organism="Aminobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_115688_length_1361_cov_4.502297"
                     /isolate="AMR_MDS_3026"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:3034609"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1361)
                     /locus_tag="P5201_13930"
     CDS             complement(<1..>1361)
                     /locus_tag="P5201_13930"
                     /inference="COORDINATES: protein motif:HMM:NF012433.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004866 - endopeptidase inhibitor activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="alpha-2-macroglobulin family protein"
                     /protein_id="HRV99692.1"
                     /translation="KEYHLDIALLSPERTLPDEPLKVNGTLTDGSGAPRKGEVVLFLV
                     DEGILSLTGYRTPDPWAFFMAKRAQGVSVYDLYDQLLPLESRETPLLKAGGGAGEEAM
                     AALRAGMSPVSARTFRMLSLFLGSVPTDENGAFEATFDVPEFSGKGRLMAVASAGKSF
                     GKGEKFVAIARDITTELSLPRAVSPGDTFHAPLKIFSSASGEKQVSVKVGIDGPLALT
                     GKKEFVANLAAGGEALFSVPFKAGDEAGTTYLTVETQWEDGSFTQNLDLPVRPAFPRI
                     ALSGSGVVRGGGQVSIEIPRDWFPGTEEGRLLLSDLPMLDLAGAASFLLQYPYGCLEQ
                     TVSGAWPLLVLGDIVRDVDPLLVNEEEQKSALALRIRQISAMQLYSGGFAMWPGSSIP
                     SMWGSLYAAHFLVEAKKGGAPVPDDLLDSALSNVRSMLPLAPANESDVAYRENMTLKA
                     YAA"
BASE COUNT          256 a          435 c          425 g          245 t
ORIGIN      
        1 acgccgcata cgctttcagg gtcatgttct cccggtacgc aacgtcgctc tcgttcgcag
       61 gagccagagg cagcatgctc cggacgttgg agagggcgct gtcgagcagg tcgtcgggaa
      121 cgggagcgcc gcccttcttc gcctccacaa ggaagtgcgc ggcgtacaga gaaccccaca
      181 tcgagggaat cgagctcccc ggccacatgg cgaaaccgcc cgagtagagc tgcatcgcgg
      241 aaatctgacg gattcggagc gcgagcgccg atttttgctc ctcctcgttc acaaggagag
      301 gatccacgtc gcgtacgata tcgccgagga cgaggagcgg ccatgcgccc gaaaccgtct
      361 gttcaaggca cccgtacgga tactgcagaa ggaaggaggc cgcgcccgcg agatcgagca
      421 tcggaagatc ggagaggaga agccgcccct cctcagttcc ggggaaccag tcccttggaa
      481 tctctatcga tacctgtcct ccgccgcgca ccacgcccga acccgagagc gcgattctcg
      541 ggaaggcggg acgcaccggc agatcgagat tctgtgtgaa agagccgtct tcccactgcg
      601 tttcgaccgt caggtaggtc gttcccgctt cgtcgccggc cttgaacggc acgctgaaaa
      661 gcgcttcgcc tccggcggca aggtttgcaa cgaactcttt ctttcctgtg agcgcaaggg
      721 gaccgtcgat ccccaccttc accgagacct gcttctcgcc ggacgctgag gagaatatct
      781 tcagcggagc gtggaacgtg tctccggggg agacggcgcg cggcaacgag agttccgtgg
      841 tgatgtcgcg cgctatcgcg acgaacttct ctcccttgcc gaacgatttt ccagcggagg
      901 cgacagccat gagccgtcct ttgccgctga actcgggcac gtcgaacgtc gcctcgaaag
      961 cgccgttttc atcggtcggg acgctcccaa ggaagagcga gagcatcctg aacgtccgtg
     1021 cggagaccgg ggacattccc gcgcgaagcg ccgccatggc ctcttcgcct gcgccgccgc
     1081 ccgccttgag gagcggcgtc tctcttgatt ccaacgggag cagctggtcg tacagatcgt
     1141 agaccgaaac tccctgcgcg cgtttcgcca tgaagaaggc ccacgggtcc ggcgtcctgt
     1201 accctgtgag cgagaggatt ccctcgtcta caaggaagag gaccacctcg cccttgcggg
     1261 gcgctcccga accgtcggtc agcgtgccgt tcactttcag cggttcgtcc gggagcgtgc
     1321 gttcggggct cagcagagcg atgtcgagat ggtattcctt a
//
LOCUS       DAOTIL010000857         1361 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminobacteriaceae bacterium isolate AMR_MDS_3026
            NODE_115746_length_1361_cov_2.376723, whole genome shotgun
            sequence.
ACCESSION   DAOTIL010000857 DAOTIL010000000
VERSION     DAOTIL010000857.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868050
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminobacteriaceae bacterium (wastewater metagenome)
  ORGANISM  Aminobacteriaceae bacterium
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae.
REFERENCE   1  (bases 1 to 1361)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1361)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:19:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,224
            CDSs (total)                      :: 3,198
            Genes (coding)                    :: 3,184
            CDSs (with protein)               :: 3,184
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1361
                     /organism="Aminobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_115746_length_1361_cov_2.376723"
                     /isolate="AMR_MDS_3026"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:3034609"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..526)
                     /locus_tag="P5201_13935"
     CDS             complement(<1..526)
                     /locus_tag="P5201_13935"
                     /inference="COORDINATES: protein motif:HMM:NF015164.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015420 - ABC-type vitamin B12 transporter
                     activity [Evidence IEA];
                     GO_function: GO:0048472 - threonine-phosphate
                     decarboxylase activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CobD/CbiB family cobalamin biosynthesis protein"
                     /protein_id="HRV99693.1"
                     /translation="MEVFVVLFAAAVWDLLLGEPSSKLHPVVWMGKYIGAFWNRRPSG
                     ADDRKMLIFGGVLVVSGALLFSLPLRFLKLLPGLTALLLTIPLLKSVFSISALINAGE
                     SILRALELGDVPEARRLLSWSLVSRNTEDLSQEEVVGAAIESLAENLTDSLASPLFFY
                     AIFGLPGAFFYRFCN"
     gene            complement(553..>1361)
                     /locus_tag="P5201_13940"
     CDS             complement(553..>1361)
                     /locus_tag="P5201_13940"
                     /inference="COORDINATES: protein motif:HMM:NF020133.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRV99694.1"
                     /translation="LDGVQCLVSCPVDRMARLELLLEEGEGISVPPEMSKTRRALELA
                     LARRPLAGALRVRRLEALPEGKGYASSTADILAALYGLANLSGTPLTPEEASRIALAV
                     EPTDSIAWSGLALLDHREGRVMQFLGPPPPLAVLVVDEGGVVDTEEFNRRDSRALLAS
                     LVSTHREAFALLKEGVERGDPAEVGEAATLSAEACRRVLEKPLLERCRALVRSLGGYG
                     VCVAHSGTLYGLLLPVALLNEKEHVTEAAARALPEAAEIRLHALAAGGPR"
BASE COUNT          273 a          480 c          417 g          191 t
ORIGIN      
        1 tgttgcagaa tcggtaaaag aaggcccccg gaaggccgaa gatcgcatag aagaagagcg
       61 gcgaagccag gctgtcggtg aggttttcgg ccagcgactc tatcgccgcg ccgacgacct
      121 cctcctgcga cagatcctcc gtgttccggc tgaccaggct ccacgaaagc agccgccggg
      181 cctccggcac atcgccgagt tcaagcgccc gcaggatgct ctctcctgcg ttgatcagcg
      241 cggagatgga aaagacgctc ttcagcagcg gaatggtgag cagcagcgcc gtcaaccccg
      301 gaaggagctt caaaaaacgc agcggaaggg agaagagcag cgcccccgac accacgagca
      361 cgccgccgaa gatgagcatc ttccggtcgt ccgcaccaga cggacggcgg ttccagaacg
      421 cgccgatgta ctttcccatc cagaccaccg gatgcagttt cgacgagggc tccccaagca
      481 ggagatccca cactgcggcg gcgaagagaa cgacaaacac ttccatgcga ataccttcct
      541 ttcgaacgga ttctaccgcg gtcccccggc cgccagggcg tgaagacgaa tctccgccgc
      601 ctccggcagc gcgcgcgcgg cggcctccgt tacatgctct ttttcattca gcagggcgac
      661 ggggaggagc agaccgtaga gcgtgccgct gtgcgcaacg cagacgccgt accccccgag
      721 cgaacgaacg agcgcgcggc accgctccag cagcggtttt tccagaacgc ggcggcacgc
      781 ctccgcgctg agcgttgcag cctcccccac ttcggcgggg tctccccgct ccacgccctc
      841 cttcaacagg gcgaacgcct ccctgtgcgt ggaaacgagc gaagcgagaa gagcgcggct
      901 gtccctccgg ttgaactcct cggtgtccac gacgcccccc tcgtccacca caagcacggc
      961 gagaggcggc gggggaccga ggaactgcat gacgcgcccc tcccgatggt cgagcagcgc
     1021 caagccggac cacgcgatac tgtccgtcgg ttcgacggca agcgcgattc gcgacgcctc
     1081 ctccggcgtc agcggcgtcc ccgaaaggtt cgccaagccg tagagcgcag caagaatatc
     1141 cgccgtgctg ctggcatacc cctttccctc cggaagcgcc tcaaggcggc gaacccgcag
     1201 cgcgccagcg agagggcgac gggcaagcgc cagttcgagc gctctccgcg tcttgctcat
     1261 ctcgggcgga accgaaatgc cttccccctc ttcaagcagc agttccagcc gggccatcct
     1321 gtcgacgggg caggagacaa ggcactgcac gccgtcgagc g
//
LOCUS       DAOTIL010000858         1357 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminobacteriaceae bacterium isolate AMR_MDS_3026
            NODE_116204_length_1357_cov_2.802611, whole genome shotgun
            sequence.
ACCESSION   DAOTIL010000858 DAOTIL010000000
VERSION     DAOTIL010000858.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868050
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminobacteriaceae bacterium (wastewater metagenome)
  ORGANISM  Aminobacteriaceae bacterium
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae.
REFERENCE   1  (bases 1 to 1357)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1357)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:19:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,224
            CDSs (total)                      :: 3,198
            Genes (coding)                    :: 3,184
            CDSs (with protein)               :: 3,184
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1357
                     /organism="Aminobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_116204_length_1357_cov_2.802611"
                     /isolate="AMR_MDS_3026"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:3034609"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..834
                     /locus_tag="P5201_13945"
     CDS             <1..834
                     /locus_tag="P5201_13945"
                     /inference="COORDINATES: protein motif:HMM:NF019344.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HRV99695.1"
                     /translation="PTGTGKTELAKALAEAFFGSVRYMVRLDMSEYSDDASVSRLLGS
                     NPGYIDSERGGLITEAVRKNPFSLFLFDEIEKAAPTVIRLFLQILDEGRLADASGESF
                     DFKNVLMVFTTNAASHGRKRSLGFLGREDALVQEREIMEQELQEVFPREFLGRFDELV
                     MFDELSPETVREIFVSIIAEETVHLSEKGVFLEVGEDAIDALSVLAAGSPFGGREVHK
                     VLAAKVLDPLSELILEGARGVFKVEVSSAEGKEIRVRPAHRCGETHEGRSEGSLRSKA
                     G"
     gene            840..>1357
                     /locus_tag="P5201_13950"
     CDS             840..>1357
                     /locus_tag="P5201_13950"
                     /inference="COORDINATES: protein motif:HMM:NF012376.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HRV99696.1"
                     /translation="MKIFITGDIHGKLDFEKLQPRFFPQGPSLSEEDVLLVAGDFGLP
                     VLPAHSEADYYLDDLASRKYTTLFIDGNHEHFGFLASLKTEYRFEGPVGVLRPNVLHL
                     RRGNVYEIGGLSLFAMGGARGMSNMAGYGFDSRETPSEREWERAHKSLEEREWTVDFV
                     VTHAAPARFLPHL"
BASE COUNT          262 a          364 c          442 g          289 t
ORIGIN      
        1 cccaccggta cgggaaagac ggaactcgcc aaggcgctcg ccgaggcttt tttcggctcg
       61 gtgcgatata tggtgcgcct cgatatgagc gagtactcgg atgatgcgag cgtcagccgc
      121 cttttggggt ccaatccggg gtatatcgat tcggaaaggg gcggcctgat tacggaagca
      181 gtgcggaaga atcctttctc gttgttcttg ttcgatgaga tagagaaggc cgcgcccacc
      241 gtgatacgcc tctttttaca gattctggac gaggggcgtc tggcggatgc ctcaggggaa
      301 tcgttcgact ttaaaaacgt gcttatggtg tttacaacga acgccgcatc gcacgggcga
      361 aaacgctccc tggggtttct cggcagggag gacgctctgg tgcaagagcg cgagataatg
      421 gagcaggagc ttcaggaggt ttttccaagg gagtttttgg ggcgtttcga cgagctggtc
      481 atgttcgacg aactctctcc ggagacggtg cgcgagatct tcgtttccat catagcggaa
      541 gaaacggtgc atctttcgga aaaaggagtg tttctggagg tcggcgaaga tgcgattgac
      601 gcgctttccg ttctggccgc cgggagtccc ttcggcgggc gggaggtcca caaggtgttg
      661 gcggcgaaag ttttggatcc tctctccgag ctgatccttg agggcgcgcg tggcgtgttt
      721 aaggtggaag tctcttcagc ggaagggaag gaaatacgcg tccgccccgc ccatcggtgc
      781 ggcgaaacgc acgaaggccg gagcgagggc tctttgagat cgaaagcggg gtaaactcaa
      841 tgaaaatctt catcaccggc gacattcacg gcaagctcga tttcgagaag ctgcaaccgc
      901 gattctttcc tcaggggccg tccctctcgg aggaggacgt gctgctggtc gccggtgatt
      961 tcggcctgcc cgttctgccc gcacacagcg aagcggacta ctatctcgac gatctcgcct
     1021 cgcggaagta cacgacgctc ttcatcgacg gcaatcacga gcacttcgga ttccttgcct
     1081 cgctgaaaac ggaataccgc ttcgaaggtc cggtcggcgt gttgcggccg aacgtcctcc
     1141 acctcaggcg cggcaacgtc tacgagatcg gcggcctctc cctcttcgct atgggcgggg
     1201 cgcgcgggat gagcaacatg gccgggtacg ggttcgactc gcgggagact ccttcggaga
     1261 gagagtggga gcgtgcgcac aaatcgctgg aggaacggga gtggacggtc gatttcgtcg
     1321 tgacccacgc ggctccggcc cgctttcttc cgcatct
//
LOCUS       DAOTIL010000859         1356 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminobacteriaceae bacterium isolate AMR_MDS_3026
            NODE_116308_length_1356_cov_4.186779, whole genome shotgun
            sequence.
ACCESSION   DAOTIL010000859 DAOTIL010000000
VERSION     DAOTIL010000859.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868050
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminobacteriaceae bacterium (wastewater metagenome)
  ORGANISM  Aminobacteriaceae bacterium
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae.
REFERENCE   1  (bases 1 to 1356)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1356)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:19:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,224
            CDSs (total)                      :: 3,198
            Genes (coding)                    :: 3,184
            CDSs (with protein)               :: 3,184
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1356
                     /organism="Aminobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_116308_length_1356_cov_4.186779"
                     /isolate="AMR_MDS_3026"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:3034609"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..774
                     /locus_tag="P5201_13955"
     CDS             <1..774
                     /locus_tag="P5201_13955"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0052621 - diguanylate cyclase activity
                     [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor domain-containing diguanylate cyclase"
                     /protein_id="HRV99697.1"
                     /translation="PEMRRMEGWERLIHPDDIEKVRYKKQKCLAGDTQGYRVEYRVPG
                     GDGTVRWILERARVVLRDDARKPLRIAGTHSDVTERRELEDQIRFQATHDYLTGLPNR
                     YLFYDRFSQAIAYARRKKRKVSLLFIDLDNFKVVNDTYGHDVGDDLLKEAAERLRKVF
                     REMDTICRLGGEEFTVILPETGDRAEVQRIAERLLEFFDTPFPMNSGSIRLQISVGIS
                     IFPDDGDDIEMLLKKADLAMYRTKKHQRSAFAFAADVHD"
     gene            936..>1356
                     /locus_tag="P5201_13960"
     CDS             936..>1356
                     /locus_tag="P5201_13960"
                     /inference="COORDINATES: protein motif:HMM:NF021387.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050043 - lactate racemase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lactate racemase domain-containing protein"
                     /protein_id="HRV99698.1"
                     /translation="MRVSIPYGTSALSCDIPDSRLKAVLVSSLHELKADAPERQIVRK
                     SLENPIGSLGLRELAEGKKNVVIISSDHTRPVPSAIIMPLLLEEVRAGNPDAEITILV
                     STGGHRATTPGELLKKYGTEIVKNERIVVHDSHDDASL"
BASE COUNT          330 a          371 c          382 g          273 t
ORIGIN      
        1 ccggagatgc gtcggatgga ggggtgggaa cggctcattc accccgacga catcgagaag
       61 gtccggtaca agaagcagaa gtgccttgcc ggagatacgc aggggtacag agtcgagtac
      121 cgcgtgccgg gcggcgacgg gactgtcagg tggattttgg agcgcgcccg cgttgttctc
      181 agggacgacg cgagaaaacc gcttcggata gccggcaccc actccgacgt caccgaacgg
      241 cgggagctgg aggaccagat ccgcttccag gcgacgcacg actatctgac cgggctgccg
      301 aaccgctatc ttttttatga tagattctcc caggcgattg cgtatgcacg ccgaaaaaag
      361 agaaaggtct cgctgctctt tattgacctg gacaatttta aggtcgtcaa cgatacgtac
      421 ggtcatgatg tcggcgacga cctgctgaag gaagccgcag aacgtctgag aaaggtcttc
      481 cgggagatgg ataccatctg ccgccttggc ggtgaagaat tcacagtgat tcttcccgag
      541 acgggggacc gcgctgaagt acagcgcatt gcggagcggc tcctggagtt cttcgatact
      601 cccttcccca tgaattcagg ttcgatacgg ctccagatat ccgtggggat cagcatcttc
      661 ccagatgacg gcgacgatat cgagatgctc ctgaaaaagg cggacctcgc catgtaccgc
      721 acgaagaaac accaaagaag cgcgtttgcc tttgcggcag acgtgcatga ttgaccagcc
      781 tttcaccgga aactcaaaaa ggaaaacgcc ctaataaagt caattcttgc atgcgcctct
      841 tgacctcttg ttcaattgtc ttaataatgt ttattggaat tttcaaaaag tagcgtgcgg
      901 cagcagaatg atttttcaac aggaggcgag tgcacatgcg agtatccatt ccgtacggaa
      961 cgtcggctct ttcctgcgat atacccgaca gccgtttgaa agcggttctc gtttcgagtc
     1021 ttcacgaact gaaagccgat gcgccggagc gccaaatcgt cagaaaatcc ctcgaaaacc
     1081 cgatcggatc cctcggcctg cgagagctcg cggagggaaa aaagaacgtc gtgattatct
     1141 caagcgacca tacccgcccc gttccaagcg ccattatcat gccgctgctc ctcgaagagg
     1201 tgcgggcggg gaaccccgat gccgaaatca ccatccttgt ctctaccgga ggacaccgcg
     1261 ccaccacccc aggggagctc ctgaagaagt atgggaccga gattgtgaag aacgagcgca
     1321 ttgttgtcca cgacagccat gacgatgcga gtctcg
//
LOCUS       DAOTIL010000860         1354 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminobacteriaceae bacterium isolate AMR_MDS_3026
            NODE_116571_length_1354_cov_3.213241, whole genome shotgun
            sequence.
ACCESSION   DAOTIL010000860 DAOTIL010000000
VERSION     DAOTIL010000860.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868050
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminobacteriaceae bacterium (wastewater metagenome)
  ORGANISM  Aminobacteriaceae bacterium
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae.
REFERENCE   1  (bases 1 to 1354)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1354)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:19:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,224
            CDSs (total)                      :: 3,198
            Genes (coding)                    :: 3,184
            CDSs (with protein)               :: 3,184
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1354
                     /organism="Aminobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_116571_length_1354_cov_3.213241"
                     /isolate="AMR_MDS_3026"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:3034609"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..217)
                     /locus_tag="P5201_13965"
     CDS             complement(<1..217)
                     /locus_tag="P5201_13965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008523263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium chelatase domain-containing protein"
                     /protein_id="HRV99699.1"
                     /translation="MNGVQGITLRGIEAVAVEVEAEITGGLFSIAIVGLPDAAVRESK
                     ERVRAALRFLGMAVRGRVAVNLAPADLP"
     gene            complement(214..588)
                     /locus_tag="P5201_13970"
     CDS             complement(214..588)
                     /locus_tag="P5201_13970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005659012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004518 - nuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YraN family protein"
                     /protein_id="HRV99700.1"
                     /translation="MTAEHLLTGRRGEELAEAHLRAVGWTILERNVTFRGGELDIVAR
                     DGNELVVVEVRTRAPGWMQSGEESVGPRKIRRLVRTGMKYMDSRKWDGPWRIDVVAVT
                     LPENGDAHIERFEDITAGGNFA"
     gene            complement(585..863)
                     /locus_tag="P5201_13975"
     CDS             complement(585..863)
                     /locus_tag="P5201_13975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012870093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EscU/YscU/HrcU family type III secretion system
                     export apparatus switch protein"
                     /protein_id="HRV99701.1"
                     /translation="MSPPKKKRAQAAAVQYDQKNDDAPRIVASGEGYVAEKIVELARQ
                     ADVPVVEDAALVSALLVLELGEEIPGELYEAVAKILTFVYKLDKGEKL"
     gene            complement(860..>1354)
                     /locus_tag="P5201_13980"
     CDS             complement(860..>1354)
                     /locus_tag="P5201_13980"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRV99702.1"
                     /translation="TRDELRERLARGDDPRKALRSLLGKDDDIAAFLRGHGLLSSPSR
                     EGVDPSLLAGAFLPAGDDAQPLNARIATSAFKRGEKSFWSVSFEMEGDRLGLVAGDVE
                     TDGRSLSVVLKAEQEASFEELRRRRHILRHELEEIPLSLQNVAVAHGRRMPRVPGRGL
                     DITV"
BASE COUNT          229 a          471 c          395 g          259 t
ORIGIN      
        1 tcggaagatc cgccggagca agattgaccg cgacacgccc cctgacggcc attcccagaa
       61 agcggagcgc cgcgcggacc ctttctttcg actcgcgcac cgccgcgtcg ggaaggccga
      121 cgatggcgat ggagaaaagc ccgcctgtga tctcggcttc aacctcgacg gcgaccgctt
      181 cgataccgcg gagcgtgatc ccctggacgc cgttcatgcg aaattacctc ccgccgtgat
      241 gtcctcgaac cgttcgatat gcgcatctcc gttctccggc agcgtcacgg ccaccacgtc
      301 gatccgccac ggaccgtccc atttgcggga atccatgtat ttcatcccgg tgcggacaag
      361 gcggcggatc tttctcggcc ccaccgactc ctcgccgctc tgcatccaac cgggcgcgcg
      421 cgtgcgcacc tccacgacga cgagctcgtt cccgtcgcgg gcgacgatat ccaactcgcc
      481 tcccctgaag gtgacgtttc gttcaagaat cgtccatccg acggcgcgca gatgcgcttc
      541 ggcaagctcc tcgccgcggc ggcccgtcag aaggtgctcc gccgtcatag tttttctccc
      601 ttatcgagtt tgtagacgaa ggtgagtatc ttggcgacgg cctcgtacag ctctcccggg
      661 atttcctcgc ccagttcaag gacgagcagc gcgctcacaa gcgcggcgtc ctcgacgacg
      721 ggaacgtcgg cctggcgcgc gagctccacg atcttctccg cgacgtaccc ctctccagag
      781 gcgacaatgc gcggagcatc gtcgttcttc tgatcgtact ggactgcggc cgcctgggcg
      841 cgcttttttt tcggggggct cataccgtga tgtccagtcc gcgccccggc acgcgaggca
      901 ttcgccttcc gtgggccacg gcgacgttct gcagggaaag cggaatttct tcgagttcat
      961 gacgaagaat atggcggcga cggcgaagct cctcgaagct cgcctcttgt tccgccttca
     1021 gcaccacgct caaggatcgt ccatccgtct ccacatctcc cgccacaagc ccgagccgat
     1081 ccccctccat ttcaaaggag acggaccaga acgatttttc tcctctttta aaggcgcttg
     1141 tggcaatccg ggcgttcagc ggctgcgcgt cgtctccggc ggggaggaat gcccccgcga
     1201 gcagcgacgg atccacccct tcgcgcgacg ggctggaaag aagaccgtgc ccgcgcagaa
     1261 aggcggcgat atcgtcgtcc ttaccgagca gcgatcgcag ggctttccgc ggatcgtcgc
     1321 cgcgggcgag gcgttcgcgc agttcgtccc gtgt
//
LOCUS       DAOTIL010000861         1354 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminobacteriaceae bacterium isolate AMR_MDS_3026
            NODE_116619_length_1354_cov_2.110855, whole genome shotgun
            sequence.
ACCESSION   DAOTIL010000861 DAOTIL010000000
VERSION     DAOTIL010000861.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868050
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminobacteriaceae bacterium (wastewater metagenome)
  ORGANISM  Aminobacteriaceae bacterium
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae.
REFERENCE   1  (bases 1 to 1354)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1354)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:19:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,224
            CDSs (total)                      :: 3,198
            Genes (coding)                    :: 3,184
            CDSs (with protein)               :: 3,184
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1354
                     /organism="Aminobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_116619_length_1354_cov_2.110855"
                     /isolate="AMR_MDS_3026"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:3034609"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(188..>1354)
                     /locus_tag="P5201_13985"
     CDS             complement(188..>1354)
                     /locus_tag="P5201_13985"
                     /inference="COORDINATES: protein motif:HMM:NF041518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="right-handed parallel beta-helix
                     repeat-containing protein"
                     /protein_id="HRV99703.1"
                     /translation="GKADGEETLGNGGGMRILDSYTHVTNCTFSGNQATRGGGIYVSG
                     GAPVLKDSTFSGNTADLGGGIYNEESRLTVSDCAFSDNQARSGGAILNNGRETDGAVH
                     ALQSATATITKSTFSGNNTTESGGAISNWKSSPAITDSSFSANKSNVEGGAMFNSDSS
                     PLLTSCTFASNTSENGGAISSWDSSPVIVNCTFYSNRASSNGGALLNDRSSSIVVNAT
                     FTRNRASTGGGMFNFQCSPVVANSILWGNGTEVRSEGGAVTISYSIIEGGFDGEKNLS
                     VNPLLGALHDNGGPTETCALDQKSPAIDAGLFVGSRVSGNVIVPPTDQRGTARPRGAG
                     VDIGAYEYYDEDTPPAPGGGGGGGGCRASSGSAGALLLLFPLVPFLLRRRRTAP"
BASE COUNT          248 a          391 c          430 g          285 t
ORIGIN      
        1 ttgagacgcc gcaatcaatc agacgaagat cctatcggca acctgcggaa caagggcaag
       61 gcttgaattg caaggttgca tgccggaaaa agcgcgttac ggcggaaaga acacgctctc
      121 cggaacaata ggttccggag agcgcagtga agtgcatcat gaggagtatg ttgacgagga
      181 actcttgtca gggggcggtt cgacgtcgtc gcaggaggaa cggaaccagc gggaagagaa
      241 ggagaagagc cccggcgctc ccggaggacg cgcggcatcc gccaccgcca ccgccgcctc
      301 cgggagccgg cggggtgtct tcgtcgtagt actcgtaggc gccgatgtcc actccggctc
      361 ccctgggccg tgcggttccg cgttgatccg tcggcggaac gatcacgttg cccgaaaccc
      421 tcgagcccac gaagagtccg gcgtcgattg cggggctttt ctgatccaga gcgcatgtct
      481 ccgtcggccc tccgttgtca tgcaaggctc cgagcagagg attcacagaa aggttcttct
      541 ccccgtcgaa tcctccctcg ataatgctgt acgaaatagt gacggcgcct ccctccgagc
      601 ggacttccgt gccgtttccc cagagaatcg agttggcgac gaccgggctg cactggaagt
      661 tgaacatccc gccgcctgtg gacgccctgt ttcttgtgaa ggtcgcgttg acgacgatgg
      721 agctgcttct gtcgttgagc agcgcgccgc cgttggatga tgccctgttc gagtaaaatg
      781 tacagttgac gatgacgggg ctgctgtccc agctcgagat cgctccgccg ttttcggatg
      841 tgtttgaagc gaacgtgcag gatgtcagga gcggcgaact gtcgctgttg aacatggcgc
      901 ccccctcgac gttcgacttg tttgccgaaa acgaagagtc cgtgatggcg gggctgctct
      961 tccaattgga gatggcgccg ccggactccg tggtgttgtt tcctgagaac gtcgatttcg
     1021 ttatggttgc cgtcgcgctc tgaagagcgt ggactgctcc gtctgtctca cgcccgttgt
     1081 tcaggatcgc cccgccggat cgcgcctggt tgtccgagaa tgcgcagtcg gagacggtaa
     1141 gacggctctc ttcgttgtaa atgccgccgc cgaggtctgc cgtgttgcct gaaaatgtgg
     1201 agtccttcaa gacaggagcg cctccgctga cataaatgcc gccgccgcgt gtcgcctggt
     1261 tcccggagaa cgtgcagttc gtgacgtggg tatagctgtc gagaatgcgc atgccgccgc
     1321 cgttgccgag ggtttcttca ccgtccgctt tgcc
//
LOCUS       DAOTIL010000862         1353 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminobacteriaceae bacterium isolate AMR_MDS_3026
            NODE_116672_length_1353_cov_3.852851, whole genome shotgun
            sequence.
ACCESSION   DAOTIL010000862 DAOTIL010000000
VERSION     DAOTIL010000862.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868050
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminobacteriaceae bacterium (wastewater metagenome)
  ORGANISM  Aminobacteriaceae bacterium
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae.
REFERENCE   1  (bases 1 to 1353)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1353)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:19:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,224
            CDSs (total)                      :: 3,198
            Genes (coding)                    :: 3,184
            CDSs (with protein)               :: 3,184
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1353
                     /organism="Aminobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_116672_length_1353_cov_3.852851"
                     /isolate="AMR_MDS_3026"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:3034609"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(183..1187)
                     /locus_tag="P5201_13990"
     CDS             complement(183..1187)
                     /locus_tag="P5201_13990"
                     /inference="COORDINATES: protein motif:HMM:NF016292.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate binding protein"
                     /protein_id="HRV99704.1"
                     /translation="MLLALCCLWSFPATAAEVFLVSSYHEGDMCGQPQYEAVVEALKQ
                     SGIPELSFRGYYLDSRRRVGKDIDREIDTIIADIRERKPALVFTIDDLAFARLYGEVL
                     RHPSTFLVFTGLNRAVAEYDKGASFLDEKGLPAKNITGVFEYLFMKEQFEMLEVLLQR
                     PLRKVALLHSVDPVGVILKEQIADELSRTPYAGIVEFHAAEDAESALRLADAIGADDS
                     VDAWIPVTMSVKDSEGGFLTMGDLAKEMTARIRKPDLALNVSFTELGFYGGVSVDFYQ
                     MGFQTGSMAAKLLKGLPIREIPVENAGASIIAVNRSRLRELGISIPGDFSAVVDLFLE
                     "
BASE COUNT          321 a          430 c          372 g          230 t
ORIGIN      
        1 accccgcatt gaagaggaag agaggcgcgc ttggaagagg ccctcccttg agggaggtct
       61 cctcggcaga tggaagaagc gtcaggacgc agaggaaaag aaggtacccc gctgcgacaa
      121 gatgaagaaa caccagaacc ggatccgtca tcccgccctc cttcggagcc gaaaaaaacg
      181 actcattcca ggaatagatc gacgacagcg gaaaaatcgc cgggaattga gatccccagc
      241 tcgcgcagac ggctcctgtt gacggcaatg atcgaagcgc ctgcgttctc caccggaatt
      301 tcccgtatcg gaagtccctt caggagcttg gcggccatcg agccggtctg gaaccccatc
      361 tggtaaaagt ccacgctgac gccgccgtag aaccccagct cggtgaaaga gacgttgagc
      421 gccaggtccg gcttgcggat gcgcgccgtc atctccttcg ccagatctcc catggtgaga
      481 aatccgcctt ccgagtcctt caccgacatc gtcaccggaa tccacgcgtc gacggagtcg
      541 tcggcgccga tggcatcggc aaggcgaagc gcgctctccg cgtcctccgc ggcgtgaaac
      601 tccacgatgc cggcgtaggg ggtgcgggag agctcgtcgg ctatctgctc cttcaaaata
      661 acgcccacgg gatcgaccga gtgcaggagc gccaccttgc gcagcggccg ctggaggagc
      721 acttccagca tctcgaactg ctccttcatg aagagatatt caaagacgcc ggtgatattc
      781 ttcgcaggaa gccccttctc atcaaggaag gaagcgccct tatcatactc cgcaaccgca
      841 cggttcagtc cggtgaacac aagaaaggtc gacggatggc gcagcacctc gccgtacagc
      901 cgggcgaagg caaggtcgtc aatcgtgaac acaagggcgg gttttcgttc cctgatgtcc
      961 gcgatgattg tgtcgatctc cctgtcgata tccttcccga cgcgccgccg ggagtcgaga
     1021 tagtacccac ggaaggagag ctccggaata ccgctctgct tgagcgcctc cacaaccgct
     1081 tcgtactgcg gctgcccgca catatccccc tcgtggtagc tggacaccag gaatacttca
     1141 gccgccgtag ccggaaagct ccacagacag cacagcgcaa gaagcaaaag aaaccgcctc
     1201 ccggatttcc acagtctctc ccgaacagaa gaaccaagcg cacgcttttc gttttccgca
     1261 actgacgaaa cagtcacatg cacacgctcc tttcagcgca tccggcgcac cccgctaagg
     1321 cgcgtaccaa cctgcaataa aatctaagga aga
//
LOCUS       DAOTIL010000863         1351 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminobacteriaceae bacterium isolate AMR_MDS_3026
            NODE_116941_length_1351_cov_3.605710, whole genome shotgun
            sequence.
ACCESSION   DAOTIL010000863 DAOTIL010000000
VERSION     DAOTIL010000863.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868050
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminobacteriaceae bacterium (wastewater metagenome)
  ORGANISM  Aminobacteriaceae bacterium
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae.
REFERENCE   1  (bases 1 to 1351)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1351)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:19:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,224
            CDSs (total)                      :: 3,198
            Genes (coding)                    :: 3,184
            CDSs (with protein)               :: 3,184
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1351
                     /organism="Aminobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_116941_length_1351_cov_3.605710"
                     /isolate="AMR_MDS_3026"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:3034609"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1289
                     /locus_tag="P5201_13995"
     CDS             <1..1289
                     /locus_tag="P5201_13995"
                     /inference="COORDINATES: protein motif:HMM:TIGR03604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="YcaO-like family protein"
                     /protein_id="HRV99705.1"
                     /translation="YEHLISPITGVVKELAPVRVDDDVAVVYAAGHNTALKMDRLDSL
                     RRSLRSSSSGKGASEAQARASALCEAVERYSGEWTGGELRIPRALNDWSEGEAIHPND
                     VMRFSERQYAEREVWNSRGSRFNRVPEPFDPERIVDWTPVWSLTEERHKYLPTQLLYY
                     RAPARDGDEAVYSIGCSNGAASGNTLAEALLQGFFELVERDAVAVWWYNMLSRPGVDA
                     ESFGDPWIVKLAAYYRERWGREAWALDITSDLGIPAFVAVSRRIGEREERLFFGLGCH
                     LDARIALQRAFAEMNQMLSVGSGGGEDDLAGLQDKETLSWMRTATLANKPYMAPDPSK
                     PLRTFSDYPVRHTDDFLEDIAYCRRLIEAKGMEMLALDQTRPDTGMPVIKVVVPGLRH
                     FWARFAPGRLYDVPAATGLLKEPLKEDELNPIPFFF"
BASE COUNT          244 a          442 c          444 g          221 t
ORIGIN      
        1 ggtacgaaca cctgatcagc ccgatcacgg gggtggtgaa ggagctggcg cccgtgcgcg
       61 tagacgacga cgtcgccgtc gtctacgccg ccggacacaa caccgcgctg aagatggacc
      121 ggctggattc gctcagacgc agtctgcgca gctccagctc gggaaaaggc gcctccgagg
      181 cccaggccag ggcgagcgcg ctctgcgagg ccgtcgaacg ctacagcggc gagtggacgg
      241 gcggcgaact gcgaattccg cgcgcgctga acgactggtc cgagggagag gcgatccacc
      301 ccaacgacgt catgcgcttc agcgaacgcc agtatgccga acgggaggtc tggaacagcc
      361 gcggctcgcg cttcaacagg gtgccggagc cgttcgaccc ggaacgtatc gtggactgga
      421 cgcccgtctg gtcgctcacc gaagaacggc acaaatacct gccgacgcaa ctgttatact
      481 atcgcgcccc ggcgcgggac ggcgacgagg cggtctactc catcggctgc tccaacggcg
      541 ccgcctccgg caatactctc gccgaggcct tgctgcaggg cttcttcgag ctggtggagc
      601 gcgacgccgt cgccgtctgg tggtataaca tgctctcacg tcccggagtg gacgcggaga
      661 gcttcggcga tccgtggatc gtgaagcttg cggcgtacta ccgggagcga tgggggcggg
      721 aagcgtgggc gctcgacatc acttccgacc tcggcatccc cgccttcgtc gcagtgtcgc
      781 gccgtatagg cgagcgggag gaacgcctct tcttcggact ggggtgccat ctcgacgcac
      841 gcatcgcgct ccagcgagcc ttcgcggaga tgaaccagat gctctccgtc ggctcgggcg
      901 ggggcgagga cgacctcgcc ggcctgcaag acaaggagac gctctcctgg atgcgcacgg
      961 cgacgctcgc caacaagccc tacatggcgc cggacccgtc gaaacctctg cgaacattct
     1021 ccgactaccc ggtgcggcat acggacgact ttcttgagga catcgcgtac tgccgccgcc
     1081 tgatcgaggc gaaggggatg gagatgctcg cgctggacca gacccgcccc gataccggaa
     1141 tgcccgtgat caaggtcgtc gtgccgggcc ttcgccactt ctgggcgcgc ttcgctccgg
     1201 ggaggctcta cgacgtcccc gcggcgacgg ggcttctgaa ggaaccgctg aaggaggatg
     1261 agctgaaccc gattccgttc ttcttttaat ttcgtcgatg ttcgttccaa acaacggacg
     1321 atcttcacca cgccgaaagc ggcctttcac g
//
LOCUS       DAOTIL010000864         1349 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminobacteriaceae bacterium isolate AMR_MDS_3026
            NODE_117207_length_1349_cov_3.482998, whole genome shotgun
            sequence.
ACCESSION   DAOTIL010000864 DAOTIL010000000
VERSION     DAOTIL010000864.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868050
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminobacteriaceae bacterium (wastewater metagenome)
  ORGANISM  Aminobacteriaceae bacterium
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae.
REFERENCE   1  (bases 1 to 1349)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1349)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:19:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,224
            CDSs (total)                      :: 3,198
            Genes (coding)                    :: 3,184
            CDSs (with protein)               :: 3,184
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1349
                     /organism="Aminobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_117207_length_1349_cov_3.482998"
                     /isolate="AMR_MDS_3026"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:3034609"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..592
                     /locus_tag="P5201_14000"
     CDS             <1..592
                     /locus_tag="P5201_14000"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HRV99706.1"
                     /translation="GDMRVATSNWDGELVQFATIRDISEMVHLRELLKTEAITDALTG
                     LHNRRGFMLLGEELLRKALEKESNALCFFADLDGFKSVNDTLGHEEGDKVLQESAEAL
                     RKTFRSSDILARVGGDEFAALVLHDAAGNETDIIGRLRQALDTINSRSLRSYRIVMSA
                     GISLWEPENPISLGELMSDADKKMYREKEERKKVSK"
     gene            942..>1349
                     /locus_tag="P5201_14005"
     CDS             942..>1349
                     /locus_tag="P5201_14005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013047640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate decarboxylase"
                     /protein_id="HRV99707.1"
                     /translation="MGNSDHINEKGHFLFGGWDTVELAGRFGTPLYVISEEMIRARCR
                     SVRETFLNRWENVFAVYAGKAFLPLAMCRIVQSEGLGLDLVSGGELYTALAAGFPPEK
                     TFFHGNSKTREELRFALSSGVGRIVVDSMSELET"
BASE COUNT          309 a          310 c          439 g          291 t
ORIGIN      
        1 gggcgatatg cgcgtggcga cctccaactg ggacggagag cttgttcaat ttgccaccat
       61 tcgcgatata tcagagatgg tgcaccttcg ggagctgttg aagacggaag cgattaccga
      121 cgcgcttacg gggctccaca accggagagg gttcatgctc ctcggagaag agctcctgcg
      181 gaaggctctt gaaaaggagt cgaacgccct ctgcttcttc gccgatctcg acgggttcaa
      241 gtcggtgaac gacacgctcg gtcacgagga gggggacaaa gtcctccagg agtcggcgga
      301 agcgctcaga aagaccttcc gttcttcgga tatactggca agggtcggcg gggatgaatt
      361 cgcggctctc gtccttcacg atgctgcggg aaacgaaacg gatatcatcg ggaggttgcg
      421 gcaggcgctt gatacgatca attcgcggag cctgcgatcc taccgtatcg tgatgagcgc
      481 gggaatatcg ctttgggagc cggaaaaccc catttctctc ggcgagctga tgtcggatgc
      541 ggacaagaag atgtatcgtg aaaaagagga gagaaaaaaa gtttcaaagt agcagttttt
      601 ttactgttga cgtttccgta tgtactggct ataatgtcac gaataaataa gatgtacacg
      661 cgatgatgca gattgcctgc gttcacggtt gccgccagag agagagatcc gaaggctgga
      721 aggtctcttc ggcaagggcg aaaggcgcaa agactgcgga gcgggggctg aaacggtcac
      781 gaccggaagt acgcccacgg gaggctcccg ttatagagcc gagagcggga cgcaaaaatg
      841 cgtccaagca gggtggtacc gcgagacata tggctcgtcc cttcattggg acgggccatt
      901 tttcatattt cgccgggaat gaagaaagag gtgcgcgtac tttgggcaac agtgatcaca
      961 tcaatgaaaa ggggcatttc ctcttcgggg gttgggacac ggtggagctt gccggacgat
     1021 tcggcacgcc gctgtatgtc atctcggagg agatgatcag agcgcggtgc agatctgtga
     1081 gagagacctt tctgaaccgc tgggagaacg tctttgctgt gtatgcggga aaggcgtttt
     1141 tgccgcttgc catgtgcagg attgttcagt cggaggggtt ggggcttgat cttgtctccg
     1201 gaggggagct ttacactgct ctcgcggcgg ggtttcctcc ggaaaagact tttttccacg
     1261 gcaacagcaa gacaagggag gagctgcgtt tcgctctttc ttcgggagtg gggcgaatag
     1321 tcgtcgacag catgagtgag ctggaaacg
//
LOCUS       DAOTIL010000865         1349 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminobacteriaceae bacterium isolate AMR_MDS_3026
            NODE_117251_length_1349_cov_2.466770, whole genome shotgun
            sequence.
ACCESSION   DAOTIL010000865 DAOTIL010000000
VERSION     DAOTIL010000865.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868050
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminobacteriaceae bacterium (wastewater metagenome)
  ORGANISM  Aminobacteriaceae bacterium
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae.
REFERENCE   1  (bases 1 to 1349)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1349)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:19:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,224
            CDSs (total)                      :: 3,198
            Genes (coding)                    :: 3,184
            CDSs (with protein)               :: 3,184
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1349
                     /organism="Aminobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_117251_length_1349_cov_2.466770"
                     /isolate="AMR_MDS_3026"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:3034609"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..555
                     /locus_tag="P5201_14010"
     CDS             <1..555
                     /locus_tag="P5201_14010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009201996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rubrerythrin family protein"
                     /protein_id="HRV99708.1"
                     /translation="MSVKNAMTAEFLRSAYGGESMAHMRYLGWADKADKEGFQKIGRL
                     FRAVAYAEQVHATNHFVVLNGDVADSCLTAGAIFGMKETAENLVGGIMGEEHEVEQMY
                     PVYYEAAKFQGEKDAMRTFNFALQAEKQHAELFRAARECALAKKDMEFEAVYVCPVCG
                     HTVTDEAPDKCPVCGVAKEKYARF"
     gene            642..1325
                     /gene="sdaAB"
                     /locus_tag="P5201_14015"
     CDS             642..1325
                     /gene="sdaAB"
                     /locus_tag="P5201_14015"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013047491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003941 - L-serine ammonia-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA];
                     GO_process: GO:0006565 - L-serine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase, iron-sulfur-dependent
                     subunit beta"
                     /protein_id="HRV99709.1"
                     /translation="MSFLDIVGPVMIGPSSSHTAGAARLGRLAKACWGTDTLNEVTVF
                     LRGSLAFTSKGHGTDKAVVAGLMGMLPDDERIRNALMLAKEAHFPYRLTTEDVDGAHP
                     NSARFSFLGKNGRRLDVVGASIGGGAVEIQEIDGFSVAINGELPTLITFHKDTRGVVA
                     AVAALMAGREINIASMTLHRKTRGGVATLVTELDMNAPPDEQLCNDVLQAHPAILRVA
                     SLFGGGNNS"
BASE COUNT          293 a          393 c          418 g          245 t
ORIGIN      
        1 atgtccgtaa aaaacgccat gaccgcagag tttctccgtt ccgcctacgg cggagagagt
       61 atggcccaca tgcgctacct cggctgggct gacaaggcgg ataaagaggg ctttcaaaag
      121 atcggacgac ttttcagggc agtcgcctac gccgagcagg tgcacgcgac gaatcacttc
      181 gtcgtgctga acggcgatgt cgcggactcc tgcctgacgg ccggcgccat atttggaatg
      241 aaagagacag ccgagaatct cgtcggcggg atcatgggcg aggagcacga agtcgagcag
      301 atgtaccctg tgtactacga ggccgcaaag ttccagggcg aaaaggacgc gatgcggaca
      361 ttcaacttcg cgctccaggc cgagaagcag cacgccgagc ttttccgcgc cgcccgcgag
      421 tgcgcccttg ccaagaagga catggagttc gaggccgtgt acgtctgtcc cgtgtgcggg
      481 cataccgtca ccgacgaagc gccggacaag tgcccggtgt gcggcgtagc gaaggaaaaa
      541 tacgcccgat tctagaagtt tttccaaggt agcctgttcc agggggagga gcgcagcttc
      601 ctccccctgc ttgctataac gcacggaaag ggaggcgagc catgagcttt ctcgatatcg
      661 tcggcccggt gatgataggc ccctcgtcga gtcatacggc gggagcggcc cgcctgggca
      721 gacttgcaaa ggcatgctgg gggaccgaca ctcttaacga agtaaccgtc tttctccgcg
      781 gcagtttggc cttcaccagc aaaggacacg gcacggacaa ggccgtcgtt gcgggtctga
      841 tgggaatgct gcccgatgac gaacgcatcc ggaacgcgct catgctggcg aaagaagcac
      901 actttcctta tcgcctgacg accgaggacg ttgacggggc gcaccccaac tccgcccgtt
      961 tctccttctt ggggaaaaac ggacgaagac tcgacgtcgt cggcgcttcg atcggcggag
     1021 gcgctgtcga aattcaggag atcgacggtt tctcggtcgc catcaacggc gagctgccta
     1081 cgctcatcac atttcataag gatacgcgcg gcgttgtggc ggcagttgcc gcgctcatgg
     1141 cgggacggga gatcaacatc gcctccatga cgctccatcg aaagacgcgg ggcggagtgg
     1201 caaccctcgt cacagagctc gatatgaatg cacctcctga cgagcagctg tgtaacgacg
     1261 tactacaagc gcacccggcg attctccgcg tcgcatcgct gtttggcgga ggaaacaatt
     1321 catgagatcc ctggccgaga tcttcgcag
//
LOCUS       DAOTIL010000866         1348 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminobacteriaceae bacterium isolate AMR_MDS_3026
            NODE_117400_length_1348_cov_2.326373, whole genome shotgun
            sequence.
ACCESSION   DAOTIL010000866 DAOTIL010000000
VERSION     DAOTIL010000866.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868050
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminobacteriaceae bacterium (wastewater metagenome)
  ORGANISM  Aminobacteriaceae bacterium
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae.
REFERENCE   1  (bases 1 to 1348)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1348)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:19:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,224
            CDSs (total)                      :: 3,198
            Genes (coding)                    :: 3,184
            CDSs (with protein)               :: 3,184
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1348
                     /organism="Aminobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_117400_length_1348_cov_2.326373"
                     /isolate="AMR_MDS_3026"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:3034609"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..549)
                     /locus_tag="P5201_14020"
     CDS             complement(<1..549)
                     /locus_tag="P5201_14020"
                     /inference="COORDINATES: protein motif:HMM:NF012652.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase, T2SS/T4P/T4SS family"
                     /protein_id="HRV99710.1"
                     /translation="MRIDELLAEVSVRGASDLHISEGLPPSFRVDGELRPLADFPSLS
                     REDMEEALQHLLSPEEMEKFRKSREHDFSFSFRSPDGEEARFRGNAYFESGRMGLALR
                     LIPMRIRTLDELGLPSTLKEISTHRRGLFLVTGPTGHGKSSTLAALLQMINTTRREHI
                     ITIEDPVEYVYISDKCIVHQRQV"
     gene            complement(569..>1348)
                     /locus_tag="P5201_14025"
     CDS             complement(569..>1348)
                     /locus_tag="P5201_14025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009202150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GspE/PulE family protein"
                     /protein_id="HRV99711.1"
                     /translation="DDDKQKLDYICEAPYGIVLVTGPTGSGKSTTLYSVLEQINKPEV
                     NIVTVEDPVEYTLFGIGQVQVNERAGLTFNSTLRSILRQNPDKIMVGEIRDGETAQLA
                     IRAALTGHLVLSTLHTNDAPSASIRLIDMGIAPFLVASSLLAVIAQRLVRTLCPSCKE
                     EYIIPEPVCKTLGLPIDTRGYRPVGCNDCRGTGYKGRSAIFEIMLLNESIRELIVSGA
                     SADVIRKEAIRLGMTTLRESGVRKIAAGVSSVEDVLTTTFS"
BASE COUNT          238 a          363 c          437 g          310 t
ORIGIN      
        1 cacctgtcgt tggtggacga tgcacttgtc ggagatgtac acgtactcga cggggtcttc
       61 gattgtgatg atgtgctccc tgcgggtggt attgatcatc tggaggagcg cggcgagcgt
      121 ggagcttttt ccgtggccgg tggggccggt taccaggaag agtcctctgc gatgcgtgga
      181 aatttctttg agcgtcgacg gcagtccgag ttcgtccagc gtacggatac gcatcgggat
      241 gagccggagc gccagcccca ttctgccgga ctcgaaatag gcgttgccgc gaaaacgggc
      301 ctcttctcca tcaggcgacc ggaatgagaa gctgaagtcg tgctcgcggc tctttcggaa
      361 tttctccatc tcttcggggg aaagcaggtg ttgcagcgct tcctccatgt cttccctgga
      421 gagcgagggg aaatcggcga ggggacgaag ctctccgtct acgcggaagc ttggcggaag
      481 tccttcgctg atgtgaaggt cgcttgctcc gcgtaccgaa acttcggcca gcagttcgtc
      541 tatccgcacg aggtatcctc tccttccgtc atgagaacgt cgtggtcaac acgtcttcga
      601 cactcgaaac accggcggct atcttccgta ctcccgactc tctcagcgtt gtcatgccca
      661 ggcgtatcgc ttccttccgt atgacgtcgg cgcttgcgcc gctgacaatg agttcccgga
      721 tcgattcatt gagcagcata atctcgaaaa tcgcgctccg ccctttgtag cccgttccgc
      781 ggcagtcgtt gcaccccacg ggacggtacc cgcgtgtatc gatggggagg ccgagtgttt
      841 tgcagaccgg ctctggaata atgtattcct ctttgcacga ggggcagagt gtgcgcacaa
      901 gacgctgagc gatgacggcg agcagggagg aggcgacgag gaagggggcg atccccatgt
      961 ctatgagacg gatcgacgcg cttggagcgt cgttggtgtg cagggtggag aggacaaggt
     1021 gtccggtgag ggctgcgcgt atggcaagct gggccgtttc gccgtcgcgg atttcgccga
     1081 ccattatctt gtcggggttc tgccggagaa tcgaacggag cgtggagttg aacgtcagcc
     1141 cggcgcgttc attgacctgc acctgtccga taccgaagag cgtgtactcc accgggtcct
     1201 ccactgtgac gatgttcacc tccggcttgt tgatctgttc gagtacggaa tagagggtag
     1261 tggactttcc gcttccggtg gggcctgtga cgaggacgat gccgtaggga gcttcgcaga
     1321 tgtagtcgag cttctgtttg tcgtcgtc
//
LOCUS       DAOTIL010000867         1344 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminobacteriaceae bacterium isolate AMR_MDS_3026
            NODE_117889_length_1344_cov_3.084562, whole genome shotgun
            sequence.
ACCESSION   DAOTIL010000867 DAOTIL010000000
VERSION     DAOTIL010000867.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868050
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminobacteriaceae bacterium (wastewater metagenome)
  ORGANISM  Aminobacteriaceae bacterium
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae.
REFERENCE   1  (bases 1 to 1344)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1344)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:19:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,224
            CDSs (total)                      :: 3,198
            Genes (coding)                    :: 3,184
            CDSs (with protein)               :: 3,184
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1344
                     /organism="Aminobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_117889_length_1344_cov_3.084562"
                     /isolate="AMR_MDS_3026"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:3034609"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..231)
                     /locus_tag="P5201_14030"
     CDS             complement(<1..231)
                     /locus_tag="P5201_14030"
                     /inference="COORDINATES: protein motif:HMM:NF014068.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DJ-1/PfpI family protein"
                     /protein_id="HRV99712.1"
                     /translation="MAKSVLAVLAEGFEEIEAITPVDVLRRLDVDVTVASLDGLVVKG
                     ARGISVQADTTLAEVAGRTFDLIVLPGGMPGAA"
     gene            375..1277
                     /locus_tag="P5201_14035"
     CDS             375..1277
                     /locus_tag="P5201_14035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008712533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050661 - NADP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-binding domain-containing protein"
                     /protein_id="HRV99713.1"
                     /translation="MTKERIGFIGLGIMGKRMALNLLKAGYSMTIRSGNEETRSEFAA
                     KGVRVEHTPAEVAAESDVIITMLPGSPEVEEVVLGENGIIKTVRAGSACIDTSSIDPV
                     TSVRIASELRKKGVFMLDAPVSGGQEKAESGTLSIMAGGDEEVFERCRGILQSMGKDV
                     VLVGGSGAGQIAKLVNQCIVAVNMAIVGKAMCLGKKAGVDPRKIFEAIRGGLAGSQCM
                     TDKAPRMFSGGYTPGGKMWLHVKDLKNVMTVCREYDVVMQLTESVMAMMEDVVRDWGP
                     DTDHGGVAKYFEKLNGVSLVTKDS"
BASE COUNT          301 a          391 c          427 g          225 t
ORIGIN      
        1 cgccgcccca ggcattccgc ccggcagcac tatcaggtcg aaggtccgtc cggcgacctc
       61 cgcaagcgtc gtatcggcct gaacagaaat tccccgggcc cccttcacca cgagcccgtc
      121 aagcgacgcg accgtcacat cgacatcaag ccgccgcagc acgtcgaccg gcgtaatcgc
      181 ctcgatctcc tcgaacccct cggcaagcac agcgagcaca ctcttcgcca tcttcactcc
      241 tccttcttta tgaaaccggt cggccgttcc cggcctttct ttccattata cccgagcggc
      301 ggctcgtgcc gtccattagc agatgataca atgatgaagc acagcgcgca cttcaacaga
      361 agggacggta cggcatgacg aaagaacgaa tcggattcat cggcctgggg atcatgggga
      421 agcgcatggc gctgaacctg ctcaaggcgg ggtactccat gaccatccgc agcggcaacg
      481 aagagacccg gagcgagttt gccgcaaagg gcgtccgtgt ggaacacacg cccgccgagg
      541 tggctgccga aagcgacgtg atcatcacca tgcttccggg atctcccgag gtggaggagg
      601 tcgtcctggg cgaaaacggc atcatcaaga ccgtccgagc ggggagcgcc tgcatcgaca
      661 caagttccat cgatccggtg acgtcggtac gcatcgcctc ggaactgcgg aagaaaggcg
      721 tctttatgct cgacgctccg gtcagcgggg gtcaagagaa ggcagagtcc ggcacgctgt
      781 ccatcatggc gggaggcgac gaagaggtct tcgagcgctg caggggcatt ctacagtcga
      841 tgggcaagga cgtcgtcctg gtgggcggct caggggcggg gcagatcgcc aagctggtga
      901 atcagtgcat cgtcgccgtc aatatggcga tcgtgggcaa agccatgtgc ctggggaaga
      961 aagcgggcgt ggatccgcgg aagatcttcg aggccatacg aggagggctt gccggaagcc
     1021 agtgcatgac cgacaaggcg ccgcgcatgt tcagcggggg ctacaccccc ggcggaaaaa
     1081 tgtggctcca cgtcaaggat ctgaaaaacg tgatgaccgt atgccgggag tatgatgtcg
     1141 tcatgcaact gaccgaaagc gttatggcga tgatggagga tgtggtacgg gactggggac
     1201 cggatacgga ccacggaggt gtggcaaagt atttcgagaa gctgaacggc gtctcgctgg
     1261 tgacgaagga ctcctgaagc gctgactgca aaaaagaaac gctcgacgcc gttcttgaca
     1321 ggatagtccg ccatgcgcac gcat
//
LOCUS       DAOTIL010000868         1343 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminobacteriaceae bacterium isolate AMR_MDS_3026
            NODE_118054_length_1343_cov_2.782609, whole genome shotgun
            sequence.
ACCESSION   DAOTIL010000868 DAOTIL010000000
VERSION     DAOTIL010000868.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868050
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminobacteriaceae bacterium (wastewater metagenome)
  ORGANISM  Aminobacteriaceae bacterium
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae.
REFERENCE   1  (bases 1 to 1343)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1343)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:19:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,224
            CDSs (total)                      :: 3,198
            Genes (coding)                    :: 3,184
            CDSs (with protein)               :: 3,184
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1343
                     /organism="Aminobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_118054_length_1343_cov_2.782609"
                     /isolate="AMR_MDS_3026"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:3034609"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..886
                     /locus_tag="P5201_14040"
     CDS             <1..886
                     /locus_tag="P5201_14040"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRV99714.1"
                     /translation="DGIESEPSVLFQGDAVREETIGDVWVSVKDMSPGRLELLFTERD
                     EDKEAAELNALRNGIPLKPQERRTKRLLTLWRDGVTIMLKDELGLIGKPTTKRPLTSD
                     DLLEYVPFTPDAYWPLGLVVAKYADELLGGLLTPAVLAAGPVPNGRCEDFITDCVQPY
                     EWWTGFNGGGAGAPALSSVKDCLRVPSTLGDALNLYSYTFKGKTLDVDESMSFLKFRE
                     HISPKCAEHLRRRTVKRIERIVKSDPGCKEVQDELCEVFSRAAEQGWDTEELGRKIVA
                     VRAAADEKFSESSKPPVL"
     gene            1107..>1343
                     /locus_tag="P5201_14045"
     CDS             1107..>1343
                     /locus_tag="P5201_14045"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRV99715.1"
                     /translation="MKKKGANSMVADKQTGCSTRRDAENDNVLFSSIETAMLAALDSY
                     LWKNDRDEFLGNLEMLSGALSLVKRKAAKWLDQQV"
BASE COUNT          285 a          329 c          414 g          315 t
ORIGIN      
        1 tgatggaatt gagagcgagc cgtccgtcct ttttcagggg gacgcggtgc gcgaagaaac
       61 gataggagat gtctgggtgt cggtaaagga tatgtctccc ggccgtctgg agttgctctt
      121 caccgaaaga gacgaggata aggaagcggc ggaactgaat gcactccgga acggaatccc
      181 gttgaagccg caagaacgta ggacaaagcg cctgctgacg ctgtggcgcg acggcgtgac
      241 gatcatgcta aaagacgagc tcggcctgat cggcaaaccg acgacgaaac gtccgctaac
      301 gtcggacgac cttctggagt acgttccgtt cacaccggat gcgtactggc ctcttgggct
      361 tgtggttgcg aagtacgccg atgaactgct cggcggtctg ctgactccgg cagttttggc
      421 ggccggtcct gtgccgaacg ggcgctgcga ggatttcatt actgattgcg tccaacccta
      481 cgagtggtgg accggattca acggcggcgg agcgggtgcg cccgctcttt cctcggtgaa
      541 ggactgcctg cgcgttccat ctactctagg cgatgcgctg aatctgtatt cgtacacgtt
      601 caagggaaag acgctggacg tcgacgagag catgtcgttt ttgaaattcc gagagcacat
      661 atcgcccaag tgcgccgagc atcttcgtcg gcgtacggtt aagcggatag agcgcattgt
      721 gaagtctgat ccgggctgta aggaagtaca ggatgagctt tgcgaagtct tttcccgggc
      781 tgcggaacaa ggatgggaca cggaggagct ggggagaaag attgttgccg tccgtgccgc
      841 agcagacgaa aagttttcgg agtcttcgaa gcccccggtg ctgtagcgcc gcttcttttg
      901 attcttattg catagtgccc cggtaacggc agggcggttt ccgcctcatt cacgtcctgc
      961 cgttacgttt ttcctccctt ctgtttctct gaatatgctc gcttgacggg tagatataga
     1021 tgtggtatat tatgttcatc ccttcttctc cggattttcg gcgtttcgga ggggtaagtt
     1081 ggtcttttat tcggtgagaa gtctccatga aaaagaaagg ggcgaacagc atggtcgcgg
     1141 acaagcaaac aggttgcagt acccggcggg atgcggaaaa cgataatgtg ttattctctt
     1201 cgatcgaaac ggcgatgctg gccgctctgg attcttactt atggaagaac gaccgcgatg
     1261 agtttctcgg taatctggag atgctctccg gcgcgctttc cctcgtgaaa cggaaagcag
     1321 ccaaatggct ggaccaacaa gtg
//
LOCUS       DAOTIL010000869         1341 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminobacteriaceae bacterium isolate AMR_MDS_3026
            NODE_118300_length_1341_cov_3.604977, whole genome shotgun
            sequence.
ACCESSION   DAOTIL010000869 DAOTIL010000000
VERSION     DAOTIL010000869.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868050
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminobacteriaceae bacterium (wastewater metagenome)
  ORGANISM  Aminobacteriaceae bacterium
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae.
REFERENCE   1  (bases 1 to 1341)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1341)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:19:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,224
            CDSs (total)                      :: 3,198
            Genes (coding)                    :: 3,184
            CDSs (with protein)               :: 3,184
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1341
                     /organism="Aminobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_118300_length_1341_cov_3.604977"
                     /isolate="AMR_MDS_3026"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:3034609"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1268
                     /locus_tag="P5201_14050"
     CDS             <1..1268
                     /locus_tag="P5201_14050"
                     /inference="COORDINATES: protein motif:HMM:NF033559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="IS1634 family transposase"
                     /protein_id="HRV99716.1"
                     /translation="YLILEDKNPLYRFEKWSALHKHPCGENIGSQRSSELFASISEEE
                     KHEFFKLQGRRRMEGEFWAYDITSVSSYSECLGQVQYGKNKEDDRLPQLNLALVFGET
                     SGLPFYYRKLAGNIPDVKTVRTLLAELDVLGYSKVKLVMDRGFYSEDNINALFKEHVK
                     FIIAGRMSLSFIQQNLEPLYEQFRSYDHFNDKYELYCHTVQAEWNYTQYRPYKGDTVS
                     EPRRIYVHYYYNIDKAAEEEKAFDRRLIALKSELESGKRVPEHETSYRRFFEVATTPK
                     RGVKVAVKAEAVAKAKRYFGFFALLSNETMDASTALELYRNKDLVEKAFGNLKERLNM
                     RRTLVSSEQSLEGKLFVEFIALIYLSYIKKRMQDSGLFKDYTLQGLLDKLDVIECFES
                     PGQKPVVGEILEKQRNIYTALDVPPPGSL"
BASE COUNT          330 a          369 c          362 g          280 t
ORIGIN      
        1 actacctgat cctggaggac aaaaaccctc tctaccgctt cgagaagtgg agcgctctgc
       61 acaagcaccc ctgcggagag aacatcggct cccagcggag cagcgaactc ttcgcgtcca
      121 tcagcgagga agaaaagcac gagttcttca agctgcaggg gagaaggcgt atggagggag
      181 aattctgggc ttacgacatc acctccgtct ccagctactc cgaatgcctc gggcaggtac
      241 agtacggcaa gaacaaggag gacgaccgtc tgccccagtt gaatctggcc cttgtcttcg
      301 gcgagacctc gggtctcccc ttctactacc gcaagctggc cggaaacatt cccgacgtca
      361 agactgtgag aactcttcta gcggagctgg acgtcctcgg gtactccaaa gtcaagctgg
      421 tcatggacag aggcttttac agcgaggaca acatcaacgc cctcttcaag gagcatgtca
      481 agttcatcat cgcgggcagg atgtccctct cctttatcca acagaacctg gaaccccttt
      541 acgagcagtt ccgctcctac gatcacttca acgacaagta cgaactgtac tgccatacgg
      601 tgcaggcgga atggaactac acgcaatacc ggccgtacaa aggtgatact gtttcggagc
      661 ccaggcgcat ctacgtgcac tactattaca acatcgacaa ggcggcggag gaagagaagg
      721 ccttcgaccg acgactgatc gccctcaaga gcgaactgga gtcgggaaaa agagtcccgg
      781 aacacgagac atcgtaccgc agatttttcg aggtcgcgac caccccgaaa cgaggcgtca
      841 aggtcgctgt caaggcggag gcggtcgcca aggcgaagcg ctattttggt ttcttcgctc
      901 tcctgtccaa cgaaaccatg gacgcttcca cggctcttga gctctaccgc aacaaggatc
      961 ttgtggagaa ggccttcggt aacctcaagg agcggctcaa catgaggcgt acccttgttt
     1021 cttcggaaca aagccttgaa ggcaagctgt ttgtggagtt catcgccctg atttacctgt
     1081 cgtacataaa gaagcgaatg caggattccg ggttgttcaa agactacact ctgcaagggc
     1141 ttctggacaa gcttgatgta atagagtgtt ttgaaagccc cggacagaag cctgttgtcg
     1201 gtgaaattct ggagaagcag cgcaatatct acacggctct tgatgttccc ccacccggct
     1261 cgttatgagt gggcgggaat ccaggttgat aggacgaatg tcttttccgg cgggctgtcc
     1321 tctttctcca gaggttgtga a
//
LOCUS       DAOTIL010000870         1341 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminobacteriaceae bacterium isolate AMR_MDS_3026
            NODE_118316_length_1341_cov_3.044323, whole genome shotgun
            sequence.
ACCESSION   DAOTIL010000870 DAOTIL010000000
VERSION     DAOTIL010000870.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868050
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminobacteriaceae bacterium (wastewater metagenome)
  ORGANISM  Aminobacteriaceae bacterium
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae.
REFERENCE   1  (bases 1 to 1341)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1341)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:19:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,224
            CDSs (total)                      :: 3,198
            Genes (coding)                    :: 3,184
            CDSs (with protein)               :: 3,184
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1341
                     /organism="Aminobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_118316_length_1341_cov_3.044323"
                     /isolate="AMR_MDS_3026"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:3034609"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..470)
                     /locus_tag="P5201_14055"
     CDS             complement(<1..470)
                     /locus_tag="P5201_14055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006299821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2Fe-2S iron-sulfur cluster-binding protein"
                     /protein_id="HRV99717.1"
                     /translation="MASIKAIINGKSITAESGMTILQAARANGIHIPTLCDHPALSPF
                     GACRICLVEAEKNPKLLTSCTTPITEGMIVHTNTPRVIEARKAVLELILVRHPLECFS
                     CASNGKCELQDVAYELGVKDSRFADPGDTNTSHKLEDANPFYVRDLNKCILCGR"
     gene            complement(485..>1341)
                     /locus_tag="P5201_14060"
     CDS             complement(485..>1341)
                     /locus_tag="P5201_14060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008708833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="NADH-ubiquinone oxidoreductase-F iron-sulfur
                     binding region domain-containing protein"
                     /protein_id="HRV99718.1"
                     /translation="WGKPTNINNVETWANVPRIIVQGAECFAAMGTEKSKGTKVFALT
                     GKVKNTGLVEVPMGITIREIVFEIGGGIIGDKKFKAVQIGGPSGGCLVDEHLDLAVDY
                     ESLTAAGAIMGSGGLVVLDEGNCMVDTAKFFLEFTQRESCGKCVPCREGTKKMLDILT
                     RITEGKGVPEDIDRLLYLGNQIKDASLCALGGTAPNPVLTTLKYFRHEYEAHIFDKKC
                     PAGVCQSLLRYSINTEKCIGCTKCARNCPVSCISGKVKEPHVIDQDKCIKCGACMDNC
                     PVGAVVKG"
BASE COUNT          275 a          371 c          362 g          333 t
ORIGIN      
        1 cagcgtccgc agaggatgca tttattcagg tcgcgaacgt aaaacgggtt ggcatcctcc
       61 agcttgtgcg aagtatttgt atcgccgggg tctgcgaaac gtgaatcttt gacgccaagt
      121 tcgtacgcca catcctgaag ctcgcacttg ccgttgctgg cgcaggagaa acactccagg
      181 gggtggcgga caaggatgag ctcaagtaca gcttttcgcg cctcgataac tcgtggcgtg
      241 ttcgtgtgca caatcattcc ctcggtaatg ggcgtggtgc acgaggtaag aagtttaggg
      301 ttcttctcgg cttccacgag gcaaatgcgg caggcgccga aggggctcag cgcagggtga
      361 tcacaaagag tcggaatgtg gatgccgtta gcccgggcgg cctgaagaat cgtcatcccc
      421 gactcggctg ttatcgactt tccgttgatg atcgctttta tgcttgccat tctctgatca
      481 tctcctatcc tttgactaca gcgcccacgg ggcaattatc catgcaggcg ccgcacttga
      541 tacacttgtc ctgatctatg acatgaggtt ccttcacctt gccggaaata cagctcacgg
      601 ggcaattccg ggcgcacttc gtacagccta tgcatttttc cgtgttgatg gagtacctca
      661 acaggctctg gcagaccccg gcagggcatt ttttatcgaa aatatgcgct tcgtactcat
      721 ggcggaaata cttgagcgtt gtgagcacgg ggtttggagc agttccaccg agggcgcaaa
      781 gcgaggcgtc cttgatctgg ttcccaaggt aaagaagtct gtcaatgtct tcaggtacgc
      841 ctttcccttc ggtgattcgg gtcaggatgt caagcatctt cttcgttccc tcgcggcacg
      901 gcacgcactt tccgcaggat tcgcgctgtg tgaattcaag gaaaaacttc gccgtgtcga
      961 ccatgcagtt tccctcgtcg agcacgacaa gtccgccaga acccatgatc gcgccggcag
     1021 cggtaagcga ttcgtagtct actgcaagat caagatgttc gtcgacgaga cagccgcccg
     1081 aaggacctcc gatttgcacg gccttgaact ttttgtcgcc gataataccg ccgcctattt
     1141 cgaagacgat ctcgcggatc gtgatgccca tgggcacttc gacaagtccg gtattcttga
     1201 cctttccggt gagtgcaaaa accttggttc ccttggactt ctcggttccc attgcggcaa
     1261 agcactctgc tccctggacg ataatccttg ggacgttcgc ccacgtctcc acgttgttga
     1321 tattcgtcgg cttaccccag a
//
LOCUS       DAOTIL010000871         1340 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminobacteriaceae bacterium isolate AMR_MDS_3026
            NODE_118458_length_1340_cov_2.659144, whole genome shotgun
            sequence.
ACCESSION   DAOTIL010000871 DAOTIL010000000
VERSION     DAOTIL010000871.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868050
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminobacteriaceae bacterium (wastewater metagenome)
  ORGANISM  Aminobacteriaceae bacterium
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae.
REFERENCE   1  (bases 1 to 1340)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1340)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:19:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,224
            CDSs (total)                      :: 3,198
            Genes (coding)                    :: 3,184
            CDSs (with protein)               :: 3,184
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1340
                     /organism="Aminobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_118458_length_1340_cov_2.659144"
                     /isolate="AMR_MDS_3026"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:3034609"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            69..872
                     /locus_tag="P5201_14065"
     CDS             69..872
                     /locus_tag="P5201_14065"
                     /inference="COORDINATES: protein motif:HMM:NF015867.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein GspK"
                     /protein_id="HRV99719.1"
                     /translation="MRRFDREQRIFTARMVSEVAMDAAKLLLSKHNGKSHSAEDEFFA
                     PRTFDFPDAGVSVTMRMEPLDDRIPLNNLFLPDGKTLRREMEAPWSALWQKVGAEQLE
                     NRVLDFLDEDTEPRLGGGERTGNLNRKLLSLEELLLVPDMKPETLSGFGGKPGVRALA
                     TIWSSGKINMNVAPVEVLSLLEGLDEKLAAEIVKSRTEKPLESIVDLTRNGGFPPDAV
                     PKLMNIATFKSDWFRVSFDVVFGDGEMIATRGILKKTLKSWNTVQWEEP"
     gene            1116..>1340
                     /locus_tag="P5201_14070"
     CDS             1116..>1340
                     /locus_tag="P5201_14070"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRV99720.1"
                     /translation="MEIAPLFGGRTKGGSEGVALCIWEGEVLDNEAGVSLHNNVVWPL
                     PVALASAVKGDGGAVFRNDFVCASAFFRNGL"
BASE COUNT          297 a          305 c          387 g          351 t
ORIGIN      
        1 ccgtgctgct gatttctgtt tttcttgtct ccgcgtctac cggattcgcg atctttgccc
       61 gccgtacaat gaggcgtttt gacagggaac aacgaatttt taccgcccgc atggtttcgg
      121 aagttgcaat ggatgcggcg aaacttctcc tttccaagca caatgggaaa tcacattctg
      181 ccgaggacga atttttcgct ccccgtactt ttgattttcc ggatgccggc gtttcggtta
      241 cgatgaggat ggagccgctt gacgatcgca ttcctctgaa taatcttttt ctgccggacg
      301 ggaagactct gcgaagagag atggaggcgc catggtcggc gctgtggcaa aaagttggag
      361 ctgagcagtt ggagaaccgt gtgcttgatt ttctggacga ggatactgaa cctcgtttgg
      421 gaggcgggga gcgcaccggc aatctgaaca ggaagcttct ttccttggag gagctgcttt
      481 tagtacccga tatgaaaccg gaaactctct cagggtttgg cgggaagccg ggtgtgcgcg
      541 ccctggcaac tatttggtcg tcagggaaaa tcaacatgaa cgtagcgcca gtagaggttc
      601 tttcactttt ggaagggctc gacgagaaat tggccgctga aatagttaaa agcaggacgg
      661 agaaaccttt ggaatcgatc gttgatctca cgagaaatgg agggttccct ccagatgcag
      721 ttccgaagtt gatgaacata gctacgttca aaagcgactg gtttcgggtt tcctttgatg
      781 tcgtatttgg cgatggcgag atgattgcca ctcggggtat actgaagaaa accttaaagt
      841 catggaatac agtccaatgg gaggagccgt agataggacc aatgcagatt tccacatatt
      901 ttataaagcg cccggagggc tggagtcctc ttgcgtcctc aattgagcct attggcggaa
      961 aaagagtaga atcggtaccg tctggaggag ccctgcttct cgctcccttc aaaactgcat
     1021 cgattcattc gttttccttc ccctttcgat ctgcaaacga tgtgcgaaat gcccttgctc
     1081 tcaaatttcg ccctcttctt tcaggagagg acgaagtgga gattgctcct ctcttcggag
     1141 gaaggaccaa gggcgggtcg gaaggggttg ctctgtgtat ttgggagggg gaggtccttg
     1201 ataatgaagc aggcgtgtcg ctccataaca acgtcgtatg gcctctcccg gtggctctcg
     1261 cctctgcggt gaaaggagac ggcggggcgg tattcagaaa tgattttgtc tgtgcgtccg
     1321 cgttctttcg aaatgggctg
//
LOCUS       DAOTIL010000872         1335 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminobacteriaceae bacterium isolate AMR_MDS_3026
            NODE_119140_length_1335_cov_2.366406, whole genome shotgun
            sequence.
ACCESSION   DAOTIL010000872 DAOTIL010000000
VERSION     DAOTIL010000872.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868050
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminobacteriaceae bacterium (wastewater metagenome)
  ORGANISM  Aminobacteriaceae bacterium
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae.
REFERENCE   1  (bases 1 to 1335)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1335)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:19:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,224
            CDSs (total)                      :: 3,198
            Genes (coding)                    :: 3,184
            CDSs (with protein)               :: 3,184
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1335
                     /organism="Aminobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_119140_length_1335_cov_2.366406"
                     /isolate="AMR_MDS_3026"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:3034609"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..282
                     /locus_tag="P5201_14075"
     CDS             <1..282
                     /locus_tag="P5201_14075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005661827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0032977 - membrane insertase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YidC/Oxa1 family membrane protein insertase"
                     /protein_id="HRV99721.1"
                     /translation="YLPNLLLLLGIGFLTWYQQQLTASGNPQMAMMNWFMPLFLTFIC
                     LSLPGGVLLYWGVSSLLGVVQQLSMSRKTKAEMQQKPVLFKDKPTRSGD"
     gene            449..1099
                     /gene="jag"
                     /locus_tag="P5201_14080"
     CDS             449..1099
                     /gene="jag"
                     /locus_tag="P5201_14080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006300737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_process: GO:0051510 - regulation of unidimensional cell
                     growth [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding cell elongation regulator Jag/EloR"
                     /protein_id="HRV99722.1"
                     /translation="MNDDILVLDVSSVEEARKIASAEWDLDSADLTIKVIEEEKSFFG
                     LLGRKLRVEVRPVAPLHVLRGRNTIDSLLKMMELHITSEIADENRINLSGPDAGIIIG
                     KYGETLKSIEYLLNLVSRGADEGRRIVLDSDGYRERRELSLQRLALAAARKCARKGRP
                     AHLEPMTSWERRIVHLTLKDSPDVETRSVGESPSRKVVIYPLRRSSYSGRPPRKDS"
     gene            1099..>1335
                     /locus_tag="P5201_14085"
     CDS             1099..>1335
                     /locus_tag="P5201_14085"
                     /EC_number="2.4.99.-"
                     /inference="COORDINATES: protein motif:HMM:NF013915.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0008961 -
                     phosphatidylglycerol-prolipoprotein diacylglyceryl
                     transferase activity [Evidence IEA];
                     GO_process: GO:0042158 - lipoprotein biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolipoprotein diacylglyceryl transferase"
                     /protein_id="HRV99723.1"
                     /translation="MYPVLFHIGDNPVHSYYLLWTLALTLAVVFSRRRMTCSYGIDDD
                     DARSVIIWAFIGMLLGARAGSVYDSWAIYSADPLK"
BASE COUNT          278 a          364 c          401 g          292 t
ORIGIN      
        1 tatcttccca accttctgct gctcctggga atcggattcc tcacgtggta ccagcaacag
       61 cttacagcga gcgggaatcc gcagatggcg atgatgaact ggttcatgcc gctcttcctt
      121 acctttatct gtctcagcct tcccggagga gtccttttgt actggggcgt ctcctctctt
      181 ctcggagtgg tgcagcagct gagcatgtcc cggaagacca aagcggaaat gcagcagaaa
      241 ccggtacttt tcaaggataa accgaccagg agcggtgact agctagagac gtgccggctc
      301 cgccttcgcg gagcctccag ccggcgagcg gacgcccctg attcggggtg tccgcgtacg
      361 tcgtgcagca tacagccact tcttctcctt ttcaggggaa cggagaggct ttctcccagg
      421 gagttctctg aacggaagga ggattgttgt gaacgacgac atacttgttc tggacgtttc
      481 ttcggtggaa gaggcccgga aaatagcttc agccgagtgg gatcttgact ctgctgatct
      541 gacaatcaag gtaatcgagg aggagaagag ttttttcggt ctcctcggca gaaaattgcg
      601 cgtggaggtg cgtccggtcg cccctcttca cgttctccgg ggcagaaata ccatcgatag
      661 cctgctgaag atgatggaac tgcatattac ctcggaaata gccgatgaaa acaggatcaa
      721 tctctccgga ccggatgcgg gcatcatcat cgggaagtac ggagagacgc tcaagtcgat
      781 tgagtacctg ctcaatcttg tctcaagagg agcggacgag gggcgccgca ttgttctgga
      841 cagcgacggg tacagggagc ggcgagagct gagccttcag cgcctcgcgc tggccgccgc
      901 gcgcaagtgc gcccggaagg ggagacccgc gcaccttgaa ccgatgacga gctgggaacg
      961 gcggatcgtc catcttacgc tgaaagacag cccggatgtg gaaacgaggt ccgtaggcga
     1021 atcgccctcg cgaaaggttg tcatttatcc gcttcggagg agttcgtaca gcggaagacc
     1081 cccccgaaag gattcctgat gtacccggtt ctctttcata tcggcgacaa cccggtccac
     1141 agctactacc tgctgtggac cttggctttg actctggccg tcgttttttc gcgccggaga
     1201 atgacctgtt cctacgggat agatgacgac gatgcgcgtt cggtgattat ctgggccttc
     1261 atcggaatgc ttctgggcgc gcgcgcggga agcgtctacg actcgtgggc gatctatagc
     1321 gcggatcccc tgaag
//
LOCUS       DAOTIL010000873         1333 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminobacteriaceae bacterium isolate AMR_MDS_3026
            NODE_119356_length_1333_cov_3.158842, whole genome shotgun
            sequence.
ACCESSION   DAOTIL010000873 DAOTIL010000000
VERSION     DAOTIL010000873.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868050
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminobacteriaceae bacterium (wastewater metagenome)
  ORGANISM  Aminobacteriaceae bacterium
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae.
REFERENCE   1  (bases 1 to 1333)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1333)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:19:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,224
            CDSs (total)                      :: 3,198
            Genes (coding)                    :: 3,184
            CDSs (with protein)               :: 3,184
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1333
                     /organism="Aminobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_119356_length_1333_cov_3.158842"
                     /isolate="AMR_MDS_3026"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:3034609"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..841
                     /locus_tag="P5201_14090"
     CDS             <1..841
                     /locus_tag="P5201_14090"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HRV99724.1"
                     /translation="VTPLPPERRNVALVYQEYALFPHMNVERNIRWGLRFAALPDDAH
                     VHRLIDLLKLKPLLGRSPETLSGGEQQRVALARALAVKPSVLLLDEPLSALDPSFRED
                     LRDYLAEINRGGMTLVMVSHDFGEVLSLGNRVAVISDGVLQQVGDVRSVFRAPANRSV
                     AEFVGMKNLFPASIRGTDAVTEGGATLLLGKAAASGERLLGIRPEDVVIGAEHPGDSR
                     NAFQGKVVSIVPRGMAFEVIMRTDGGMRITARVLSSALLSGGVAVGGEYWASFSPDSV
                     HVL"
     gene            875..>1333
                     /locus_tag="P5201_14095"
     CDS             875..>1333
                     /locus_tag="P5201_14095"
                     /inference="COORDINATES: protein motif:HMM:NF024921.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HRV99725.1"
                     /translation="MKQLRRLFLAAAVLLMVSGAFAAEKVVVYHAGSLAAPMAKIEAM
                     YEEANPGVDIQRESGGSAALARKITDLGGACDAFFSADYMVIEKLLRPAAADWNILFA
                     SNAIVLMYGPKSKYGEEIGAKNWTEVMLRPDVQWGHSEPDADPCGYRSLMV"
BASE COUNT          217 a          408 c          460 g          248 t
ORIGIN      
        1 cgtcacgccc ctgccgcccg agcggcggaa cgtcgcgctc gtctaccagg agtacgcgct
       61 ctttccgcac atgaacgtgg agcggaatat ccgctggggc ctccgcttcg ccgccctccc
      121 cgacgacgcg catgtacacc gtcttatcga cctgctgaag ctgaagccgc tgctcgggcg
      181 atctccggag acgctctccg ggggggagca gcagcgcgtc gcgctcgccc gcgcgctcgc
      241 cgtgaagccc tccgtcctgc tgctcgacga gccgctctcc gcgctcgatc cgtccttcag
      301 ggaggatttg cgggactacc tggcggagat caatcgcggc ggtatgacgc tggtgatggt
      361 gagccacgat ttcggcgagg tactttcgct cgggaacagg gttgccgtga tctcggacgg
      421 cgttttacaa caggtagggg atgtccggtc ggttttccgg gcgccggcga acagaagcgt
      481 cgccgagttc gtcgggatga agaatctctt cccggcgagc attcggggca cggacgccgt
      541 aacggagggg ggcgcgacgc tcctcctcgg caaggcggcg gcttccggcg aacggctgct
      601 cggcatccgc cccgaggacg tcgtcatcgg ggccgaacac cccggagact cccggaacgc
      661 gtttcagggg aaggtcgtct ccatcgttcc aagggggatg gcgttcgaag tgattatgcg
      721 cacggacggc ggcatgcgca tcacggcacg ggttctttcg tcggcgcttc tctcgggagg
      781 agtcgccgtc ggcggcgaat actgggcgag tttcagcccg gattcagtcc atgttttgta
      841 gatggtgcat catactttaa cggaggtgtt gttcatgaaa cagttacgaa ggttgttcct
      901 tgcggcagcg gttcttctta tggtctccgg cgcgttcgcg gcggagaagg tcgtcgtcta
      961 ccatgcgggg agccttgccg ctcccatggc gaagatcgag gcgatgtacg aagaggccaa
     1021 ccccggcgtg gacatccagc gcgagtcggg cggcagcgcg gcgctcgcgc ggaagatcac
     1081 cgatctcggc ggagcgtgcg acgcgttctt ctcggcggac tacatggtca tcgagaagct
     1141 gctccggcct gcggcggcgg actggaacat cctcttcgcc agcaacgcca ttgtcctgat
     1201 gtacgggccg aagtcgaagt acggcgagga gatcggcgcg aagaactgga cggaagtcat
     1261 gctgcgcccc gacgtgcagt gggggcactc cgagcccgac gcggatccct gcggctaccg
     1321 gagcctgatg gtg
//
LOCUS       DAOTIL010000874         1332 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminobacteriaceae bacterium isolate AMR_MDS_3026
            NODE_119553_length_1332_cov_2.146437, whole genome shotgun
            sequence.
ACCESSION   DAOTIL010000874 DAOTIL010000000
VERSION     DAOTIL010000874.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868050
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminobacteriaceae bacterium (wastewater metagenome)
  ORGANISM  Aminobacteriaceae bacterium
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae.
REFERENCE   1  (bases 1 to 1332)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1332)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:19:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,224
            CDSs (total)                      :: 3,198
            Genes (coding)                    :: 3,184
            CDSs (with protein)               :: 3,184
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1332
                     /organism="Aminobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_119553_length_1332_cov_2.146437"
                     /isolate="AMR_MDS_3026"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:3034609"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..912)
                     /locus_tag="P5201_14100"
     CDS             complement(<1..912)
                     /locus_tag="P5201_14100"
                     /inference="COORDINATES: protein motif:HMM:NF017085.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHAD domain-containing protein"
                     /protein_id="HRV99726.1"
                     /translation="MKERAPTLRAFAVAALVPLLEKLSSQLEDLRTEEDIECLHKARV
                     STRRLRASLSLFAPCFPSGEAKRWKKAVTRITALLGEARDLDVQMESVEEELRATGDR
                     ERPGLARLLLRLSRRREKLQRRICAALDGRDIREPLDEMLDGLREAVRKDILERDDSP
                     SFALCADEIRAHVADVLSFDGFVRNPASIKELHDLRKAVKRLRYALEIFEPLSAGLLR
                     PFVERLKALQDLLGEIHDCDVWLDFLPRFLEEEAARTLKYYGHVRVFPPLKTGILAFM
                     EKKRTLRGEDFGCFLDEWGRLGKEAFWD"
     gene            complement(909..>1332)
                     /locus_tag="P5201_14105"
     CDS             complement(909..>1332)
                     /locus_tag="P5201_14105"
                     /EC_number="2.7.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005659147.1"
                     /note="catalyzes the reversible transfer of the terminal
                     phosphate of ATP to form a long chain polyphosphate;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="RNA degradosome polyphosphate kinase"
                     /protein_id="HRV99727.1"
                     /translation="GVQILLNVRGICCLRPGVPGVSETIDVTSIVGRFLEHPRMFYFR
                     NGGDDELFIGSADLMPRNLDRRVEVLVPVREKDLRETLLRDMLDVHLADSRKSWRLLS
                     DGIYRKITPEEGKPVIDSQKIMMQRSKGWNPPERSDER"
BASE COUNT          239 a          417 c          435 g          241 t
ORIGIN      
        1 gtcccagaac gcttccttcc cgaggcgccc ccactcgtcg agaaaacagc cgaaatcctc
       61 tccccgcagc gtccgtttct tctccatgaa agcgaggatg cccgtcttca gcgggggaaa
      121 gacccgcaca tgcccgtagt acttcagcgt ccgcgccgct tcttcctcca aaaaacgggg
      181 gaggaaatca agccagacgt cgcagtcgtg aatctctccg aggaggtcct ggagggcctt
      241 cagcctctcg acgaaggggc gaagaagccc cgcggagaga ggctcgaaga tctccagcgc
      301 gtaccggagc cgcttcacgg ctttcctgag gtcgtgcagc tccttgatcg acgcggggtt
      361 ccggacaaac ccgtcgaagc tgaggacgtc ggcgacgtgc gcgcggattt cgtccgcgca
      421 gagggcgaac gagggggagt cgtcccgttc aaggatgtcc ttgcggaccg cttcccgaag
      481 gccgtcgagc atctcgtcga gaggttcgcg tatatcgcgg ccgtcgagcg cggcgcagat
      541 gcgccgctgc agcttctccc tgcgccgcga gaggcgcagc agcagacggg cgagtccggg
      601 acgctcgcgg tcgccggttg cgcgaagttc ctcttcgacg gactccatct ggacgtcaag
      661 atcccgggct tctccgagga gggctgtgat cctggtcacg gcctttttcc agcgcttggc
      721 ctctccggag gggaagcagg gagcgaagag ggagagagag gcgcggagcc gccgggtgga
      781 gaccctcgcc ttgtgaaggc attcgatatc ctcctctgtc cggaggtcct ccagctgcga
      841 ggagagtttc tccagcagcg ggacgagcgc ggcaacggcg aaggcgcgga gggttggggc
      901 gcgctccttc atcgttcgtc gctcctttcc ggaggattcc accccttact tcgctgcatc
      961 atgatcttct gcgagtcgat cacgggcttc ccctcctccg gcgtgatctt gcgatagatc
     1021 ccgtcggaca agagtctcca cgacttcctg ctgtccgcga gatgcacgtc gagcatatcg
     1081 cggagcaaag tttcacgaag gtccttctcc ctgacgggga cgagcacctc gacgcggcgg
     1141 tccaggttgc gcggcatgag gtcggcgctt ccgatgaaca gttcgtcgtc gccgccgttg
     1201 cggaagtaga acatccgagg gtgctccagg aaacgtccga cgatggaggt gacgtcgatg
     1261 gtctcgctca cgcccggaac gccggggcga aggcagcaga taccgcggac gttcaggaga
     1321 atttgaacgc cc
//
LOCUS       DAOTIL010000875         1331 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminobacteriaceae bacterium isolate AMR_MDS_3026
            NODE_119628_length_1331_cov_3.583856, whole genome shotgun
            sequence.
ACCESSION   DAOTIL010000875 DAOTIL010000000
VERSION     DAOTIL010000875.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868050
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminobacteriaceae bacterium (wastewater metagenome)
  ORGANISM  Aminobacteriaceae bacterium
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae.
REFERENCE   1  (bases 1 to 1331)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1331)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:19:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,224
            CDSs (total)                      :: 3,198
            Genes (coding)                    :: 3,184
            CDSs (with protein)               :: 3,184
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1331
                     /organism="Aminobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_119628_length_1331_cov_3.583856"
                     /isolate="AMR_MDS_3026"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:3034609"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..810
                     /locus_tag="P5201_14110"
     CDS             <1..810
                     /locus_tag="P5201_14110"
                     /inference="COORDINATES: protein motif:HMM:NF013757.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IclR family transcriptional regulator"
                     /protein_id="HRV99728.1"
                     /translation="EGDVMQGHTTDEKTTVTGTGEEKSVRSVERAFSILMCFTLDEDE
                     LTLMDIARKMHLPTTTVSRMVNTLVGLGFLEKSPGKTYSLGANLYYLGAVARNHFKLQ
                     KIVYPYMKQVRDTTKEAVSLYVLDGDARVCYEHVESLLSMRCVVRVGDRFPLWAGAAG
                     KCLLAYADPELVDRELAKAKPITPTTIISREAFLADLETIRKKGHAISYGEREEGITS
                     IAVPVFHPKNKVTTVFSVAGPSVRFTDEIIEELLPVVKAMTEEISRSLSYR"
     gene            891..>1331
                     /gene="aroQ"
                     /locus_tag="P5201_14115"
     CDS             891..>1331
                     /gene="aroQ"
                     /locus_tag="P5201_14115"
                     /EC_number="4.2.1.10"
                     /inference="COORDINATES: protein motif:HMM:NF003805.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003855 - 3-dehydroquinate dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II 3-dehydroquinate dehydratase"
                     /protein_id="HRV99729.1"
                     /translation="MKRILMLHGVNHNMFGKRDPAQYGTITLDEIDEKLRTLGGELGV
                     EVVGFQTNSEGAMCERIHQAFQEGMDGVIINAGAWTHYSYAIRDALAILTVPVAEIHM
                     SNIHAREEFRHRSVFAEVVKGQICGFGLDSYLLGLRAVVSAIGGA"
BASE COUNT          310 a          338 c          411 g          272 t
ORIGIN      
        1 gagggggatg tcatgcaagg gcatacaaca gacgaaaaaa cgaccgtgac cggaaccggg
       61 gaggaaaagt ctgtccgttc agtggaacgg gcgttttcca tcctgatgtg cttcactctc
      121 gacgaggacg agctgacgct gatggatatc gcacggaaga tgcaccttcc gacgacgaca
      181 gtctctcgaa tggtgaacac cctagtaggt ctcggcttcc tcgaaaaaag tcccgggaag
      241 acctactcgc tcggagcgaa tctgtactat ctgggggctg tcgcacggaa ccattttaaa
      301 ctgcagaaga tagtctaccc atatatgaag caggttcgcg atacgacaaa ggaggcggtg
      361 tcgctctacg ttctcgacgg ggatgctcgg gtgtgctacg aacatgtgga gagtcttctc
      421 agcatgcgtt gcgtggtccg tgtcggagac cgtttccctc tgtgggcggg agctgcgggc
      481 aagtgcctct tggcgtacgc cgaccctgaa cttgtggacc gcgaactcgc gaaggcgaag
      541 ccgataacgc cgaccacgat aatttcgagg gaggcgttcc tggccgacct tgagacgatc
      601 cgcaaaaaag gacacgcaat cagctacggg gaacgcgagg agggaattac ctccatcgcc
      661 gttcctgtgt tccacccgaa aaacaaagtg acaaccgtgt tctccgtcgc cggtccgtcg
      721 gttcgcttta ccgacgaaat tatcgaagag ctgcttcctg tggtgaaggc gatgacggag
      781 gagatttcaa ggagcctttc ctacaggtaa cgagttcttt gcttcgttgg gctccatcat
      841 agaaaacgtt cagtcgactc gaacaacgaa agggagatgg aaacagggcc atgaagagaa
      901 tactcatgct tcacggtgtg aatcataata tgttcggaaa gcgcgatccg gcgcagtacg
      961 gaacgataac gctcgacgag atagacgaga agctgcgcac gcttggcggc gagcttggcg
     1021 tcgaggttgt cggctttcag acgaattccg aaggcgcgat gtgcgaacgg attcatcagg
     1081 cctttcagga gggcatggac ggcgtcatta tcaatgcggg ggcatggacg cactacagct
     1141 acgcgattcg ggacgccttg gcgatcctga ctgttcccgt ggcggagata cacatgtcga
     1201 acatccacgc cagggaggaa ttccggcatc gttcagtctt tgcggaggtc gtaaaggggc
     1261 agatctgcgg tttcggcctc gacagctatc tgttgggcct gcgcgcggtc gtcagcgcga
     1321 taggcggcgc g
//
LOCUS       DAOTIL010000876         1327 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminobacteriaceae bacterium isolate AMR_MDS_3026
            NODE_120197_length_1327_cov_4.628145, whole genome shotgun
            sequence.
ACCESSION   DAOTIL010000876 DAOTIL010000000
VERSION     DAOTIL010000876.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868050
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminobacteriaceae bacterium (wastewater metagenome)
  ORGANISM  Aminobacteriaceae bacterium
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae.
REFERENCE   1  (bases 1 to 1327)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1327)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:19:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,224
            CDSs (total)                      :: 3,198
            Genes (coding)                    :: 3,184
            CDSs (with protein)               :: 3,184
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1327
                     /organism="Aminobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_120197_length_1327_cov_4.628145"
                     /isolate="AMR_MDS_3026"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:3034609"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..734)
                     /locus_tag="P5201_14120"
     CDS             complement(<1..734)
                     /locus_tag="P5201_14120"
                     /inference="COORDINATES: protein motif:HMM:NF015445.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_component: GO:0031317 - tripartite ATP-independent
                     periplasmic transporter complex [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_function: GO:0005488 - binding [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DctP family TRAP transporter solute-binding
                     subunit"
                     /protein_id="HRV99730.1"
                     /translation="MKMRTGCVAILLVALFASAAFGAQFTINAGIGLNDKSAQFQSLQ
                     YFKEVVEKNSDGKIEVVLFHSSQLGDDRNMMEALKMGTQEMTCPSTAPMTAFINAFKI
                     YDLPFIFANEEVADYILDGPVGKKLLDMLPDQGLVGLAYWENGFRMLTNSSRPVQSPE
                     DLKGMKIRTMENPIHLAAFRTMGANPTPMAFGELFSAMQQKVVDGQENPWGTIFLQNF
                     FEVQKYATDTGHVYSPFVLLIWKKFW"
     gene            complement(840..>1327)
                     /locus_tag="P5201_14125"
     CDS             complement(840..>1327)
                     /locus_tag="P5201_14125"
                     /inference="COORDINATES: protein motif:HMM:NF018513.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="TRAP transporter large permease subunit"
                     /protein_id="HRV99731.1"
                     /translation="SIFLSTGKTTATVLYLCGMAMCTAWLLTRARVPFELAAMVQSIT
                     DSQLGVLLMTNVLLFLVGFVMDLTPAMLILAPILLPVMQQVGVDPVYFGVIMSVNLGI
                     GLITPPVGTVLYVATGVANVKFEELVKSIAPFVVAELAVLILLIFFPQIVMFIPNLVL
                     P"
BASE COUNT          282 a          373 c          413 g          259 t
ORIGIN      
        1 tcccagaatt tcttccagat cagcaggacg aagggggagt agacgtgccc cgtgtccgtg
       61 gcgtacttct gcacttcgaa gaagttctgc aggaagatgg tgccccaggg attctcctgt
      121 ccgtcgacga ccttctgctg catggcggag aagagttcgc cgaaggccat cggcgtcggg
      181 ttggcgccca tcgtgcggaa agcggccaag tggatggggt tctccatggt gcggatcttc
      241 atccccttga gatcctcggg cgactggacg ggacgggagc tgttcgtaag catgcggaag
      301 ccgttctccc agtaggccag gccgacgagc ccctggtcgg ggagcatgtc gagcagcttc
      361 tttcccaccg ggccgtcgag gatgtaatcc gcgacctctt cattggcgaa aatgaagggg
      421 aggtcgtaga tcttgaacgc gttgatgaag gccgtcatcg gagcggtgga ggggcaggtc
      481 atctcctgcg tccccatctt gagcgcttcc atcatgttac ggtcgtcgcc gagctggctg
      541 gagtggaaga ggacgacctc gatctttccg tccgagttct tctcgacaac ctctttgaaa
      601 tactggagcg actggaactg ggcggacttg tcgttcagtc cgatgcccgc gttgatggtg
      661 aactgcgccc cgaaggccgc cgaggcgaag agcgcgacaa gaagtatggc aacgcaaccg
      721 gttctcattt tcatctgttt ctctcccttc atgttttcaa aatgtattct tcttcaaaac
      781 agtacccgtg gtcgactctg tccgcacccc cttccggatg tctgaaaaaa tgacgcgctc
      841 tacggaagca caagattcgg gatgaacatg acgatctgcg ggaagaagat gagcaggatg
      901 agcaccgcca gctccgcgac gacgaaggga gcgatggact ttaccagctc ctcgaacttt
      961 acgttcgcca cgccggtcgc cacgtagagg accgttccca ccggcggggt gatgagcccg
     1021 atgccgaggt tcaccgacat gatcacgccg aagtaaaccg gatcgacccc cacctgctgc
     1081 atcaccggaa gaaggatcgg cgcgaggatg agcatcgccg gggtaagatc catgacgaag
     1141 ccgacgagga agagaagcac gttcgtcatc aggaggacgc cgagctgcga gtcggtgatc
     1201 gactggacca tcgccgcgag ctcgaaggga acccgcgcgc gggtgagcag ccacgcagtg
     1261 cacatcgcca taccgcagag gtagagcacc gtggcggtcg tcttgcctgt cgacaaaaag
     1321 atgctgg
//
LOCUS       DAOTIL010000877         1325 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminobacteriaceae bacterium isolate AMR_MDS_3026
            NODE_120485_length_1325_cov_3.961417, whole genome shotgun
            sequence.
ACCESSION   DAOTIL010000877 DAOTIL010000000
VERSION     DAOTIL010000877.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868050
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminobacteriaceae bacterium (wastewater metagenome)
  ORGANISM  Aminobacteriaceae bacterium
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae.
REFERENCE   1  (bases 1 to 1325)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1325)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:19:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,224
            CDSs (total)                      :: 3,198
            Genes (coding)                    :: 3,184
            CDSs (with protein)               :: 3,184
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1325
                     /organism="Aminobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_120485_length_1325_cov_3.961417"
                     /isolate="AMR_MDS_3026"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:3034609"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(43..417)
                     /locus_tag="P5201_14130"
     CDS             complement(43..417)
                     /locus_tag="P5201_14130"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRV99732.1"
                     /translation="MTDHKRKNPLKELLTPCNFQVEPGTPLALFLDFLVSDDPETGAR
                     ACIDRDMKHGDFRTRSQIPSQSCVRYVAQSLYADHGKKAVTLETIREKVKNACGHRFL
                     GNFDRYREQVAPSKDTGEESAT"
     gene            complement(580..990)
                     /locus_tag="P5201_14135"
     CDS             complement(580..990)
                     /locus_tag="P5201_14135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005660084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicB family
                     antitoxin"
                     /protein_id="HRV99733.1"
                     /translation="MASRGNKDIRTYPAIFSRSGKNIAVEFPDLPGCATFGTSEAEAV
                     ARAKEALEGFLYYSERDGDVIPVPTPFDKVQVRPGEAVSLVTVRMDIVREEEANKSVT
                     KSVTLPNWLNKLAMEAKLNFSGILQEGLKKKLGV"
     gene            complement(1045..1233)
                     /locus_tag="P5201_14140"
     CDS             complement(1045..1233)
                     /locus_tag="P5201_14140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008520638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003729 - mRNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicA family
                     toxin"
                     /protein_id="HRV99734.1"
                     /translation="MKRYSSREIIKILEQDGWFLLGARGDHHYFKHKTKPGKIPVPHP
                     EKDVDPKTAKSIFKAAGL"
BASE COUNT          262 a          357 c          360 g          346 t
ORIGIN      
        1 ccggtttttc ctcgtttcga tattccggcg aagaatccga acctacgtgg cggattcttc
       61 gccggtgtcc tttgaaggag cgacctgttc gcgataccgg tcgaaattcc ccagaaaacg
      121 atgcccgcag gcatttttca ccttttcgcg gatcgtctcg agggtgacgg cttttttccc
      181 gtggtccgcg tacaggctct gggcgacata tctgacgcag ctctgggatg gtatctggga
      241 gcgtgttctg aaatccccgt gcttcatgtc ccgatcgatg cacgcccggg ctcccgtctc
      301 cggatcgtcg gaaaccagga aatcaaggaa gagtgccagg ggagtgcccg gttccacctg
      361 gaaattgcag ggggtcagca gctctttcag cgggtttttt cttttgtgat ccgtcatctc
      421 tgactcgcct ccgtgctccg ggggtgtgcc ggctgttcgg gtgtaatatt gggtacagag
      481 tattatacac aggacggacg tacaaaagga gtactgagct tctccagaaa agacagcgcc
      541 cttaccggac attttccggg caggggcgct gtcatgcggc tacacgccga gttttttctt
      601 caagccctcc tggagtattc cggaaaagtt gagtttggcc tccatagcga gcttgttcag
      661 ccagttgggg agggtaacgc ttttcgtgac gcttttgttt gcttcctcct cacggacgat
      721 gtccatgcga acggtaacga gggatacggc ttctccgggg cgcacctgca ccttgtcaaa
      781 aggagtgggg acagggataa cgtcgccgtc acgctccgag tagtacaaga aaccttcgag
      841 agcctctttg gcacgtgcga ccgcttcagc ttccgacgtg ccgaaggttg cgcatccggg
      901 aaggtcgggg aactcaaccg ctatattttt gccgctgcgg gagaatatcg ccggatatgt
      961 gcggatatcc ttgtttcctc tactggccat agctattcct cctttcgtca tgggaacaga
     1021 atgtattgct tcaatgggcg gaacttacag ccccgccgcc ttgaaaatgc tcttggcagt
     1081 cttggggtct acatctttct caggatgagg aaccgggatc tttcccggct ttgtcttgtg
     1141 cttgaaatag tggtggtctc ccctggcacc cagcagaaac cagccgtcct gctccagaat
     1201 cttgatgatc tccctgctgc tgtaacgttt cattccccct cctcccccat cgacaacaat
     1261 atgataacgc gtgttaaaac aagtgtcaat gattatggaa gtctttgctt aagccaaaat
     1321 tttcg
//
LOCUS       DAOTIL010000878         1323 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminobacteriaceae bacterium isolate AMR_MDS_3026
            NODE_120769_length_1323_cov_3.234227, whole genome shotgun
            sequence.
ACCESSION   DAOTIL010000878 DAOTIL010000000
VERSION     DAOTIL010000878.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868050
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminobacteriaceae bacterium (wastewater metagenome)
  ORGANISM  Aminobacteriaceae bacterium
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae.
REFERENCE   1  (bases 1 to 1323)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1323)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:19:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,224
            CDSs (total)                      :: 3,198
            Genes (coding)                    :: 3,184
            CDSs (with protein)               :: 3,184
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1323
                     /organism="Aminobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_120769_length_1323_cov_3.234227"
                     /isolate="AMR_MDS_3026"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:3034609"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            295..873
                     /locus_tag="P5201_14145"
     CDS             295..873
                     /locus_tag="P5201_14145"
                     /inference="COORDINATES: protein motif:HMM:NF024671.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Panacea domain-containing protein"
                     /protein_id="HRV99735.1"
                     /translation="MIPFREQKIKNAIAYFASEFYARRCYYPRQTWIYKFLALLDYRM
                     LRKTGIPCLGLEYYAMQYGPVPVRLYDQRFDLKTDKFQFIRTDDGGSRVEPCGTPDLD
                     FFSDDELDVMDAILSVYAGTDLETLINDAHKEIRAWNKAWEIAQKVGRGRMPMEYADE
                     FTDLAGEDLTPEEEHFLCYLDMLAIEAAESAS"
     gene            880..>1323
                     /locus_tag="P5201_14150"
     CDS             880..>1323
                     /locus_tag="P5201_14150"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRV99736.1"
                     /translation="MLQLEVGDVFTWEQYPYHIEGEPKRRWFVYLGEYKENPDPFEGN
                     VMIIAPTTTTQIHYYEPRESRARNPHVRFTPEDGFGFAAESVLDLSNEHIVVAKSVFR
                     SNETSINVRGKVSHARLRLIYEKIRASTGYSPKSKAQIYENLKHAG"
BASE COUNT          338 a          328 c          340 g          317 t
ORIGIN      
        1 ccgggaatca cagtgcctcc ggcgttctgt atgcacccgc cgttctttcc cattcgaatc
       61 acttcgaatc acggttcttg tcaaaaatgg aaagaaaggg gtgctgtagc tccgcctcct
      121 tacacgaaca gccgcaggcg gaccgtctcc tccagccgct tcttcagcgc ccttcacttg
      181 ggggtcacgc cttccagcag ctcgtaggag ttattggacg tcacagtctc aatgcggact
      241 ggacgttctc ctctcggaga gagataatag catcggatgg aaggaggcgg agaaatgatt
      301 cctttccggg aacagaagat caaaaatgcc attgcgtact ttgcgtcgga gttctacgca
      361 cgtcgttgtt attatcctcg gcagacgtgg atatacaagt ttttggccct tctcgattat
      421 cgtatgctgc gaaaaacggg tataccttgt cttggcctcg aatattacgc aatgcagtac
      481 ggccctgtgc ctgtacggct ttacgaccaa cgtttcgatt tgaagaccga caagttccag
      541 tttatccgaa cagacgacgg aggaagcaga gtggagcctt gcggtacacc cgatcttgac
      601 tttttctccg acgacgagct ggacgtaatg gacgctatcc tttccgtgta cgcaggaact
      661 gatttggaaa cccttattaa cgacgcgcat aaagaaatac gggcgtggaa caaagcatgg
      721 gaaatcgcgc agaaagttgg aagaggcagg atgcctatgg agtacgccga tgaattcacc
      781 gaccttgccg gggaggatct tacacctgaa gaggagcatt tcctctgtta tctcgatatg
      841 ctcgccatag aagcggccga atcggcgtcc tgaccgaaaa tgcttcagtt ggaagtgggg
      901 gatgttttta cctgggagca gtatccgtac catatcgagg gtgaaccaaa acgccgctgg
      961 ttcgtctact taggggagta caaagaaaat ccggatccct tcgagggtaa tgttatgatc
     1021 atcgctccga caaccaccac acagatccat tattacgagc ctcgggaatc ccgcgcccgt
     1081 aatccccatg ttcggtttac tccggaagac ggtttcggat tcgctgcaga gagtgttctt
     1141 gatctttcca atgagcatat agttgttgcg aagagcgttt tcaggagcaa cgaaacgagt
     1201 atcaatgtgc gaggaaaagt gtcccacgca cgtctcagac tgatatacga aaaaatcaga
     1261 gcttctacgg ggtattctcc gaaatccaaa gcacagattt acgaaaattt aaaacatgcc
     1321 gga
//
LOCUS       DAOTIL010000879         1316 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminobacteriaceae bacterium isolate AMR_MDS_3026
            NODE_121762_length_1316_cov_2.582078, whole genome shotgun
            sequence.
ACCESSION   DAOTIL010000879 DAOTIL010000000
VERSION     DAOTIL010000879.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868050
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminobacteriaceae bacterium (wastewater metagenome)
  ORGANISM  Aminobacteriaceae bacterium
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae.
REFERENCE   1  (bases 1 to 1316)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1316)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:19:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,224
            CDSs (total)                      :: 3,198
            Genes (coding)                    :: 3,184
            CDSs (with protein)               :: 3,184
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1316
                     /organism="Aminobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_121762_length_1316_cov_2.582078"
                     /isolate="AMR_MDS_3026"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:3034609"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..429)
                     /gene="hisI"
                     /locus_tag="P5201_14155"
     CDS             complement(<1..429)
                     /gene="hisI"
                     /locus_tag="P5201_14155"
                     /EC_number="3.5.4.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008709430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004635 - phosphoribosyl-AMP
                     cyclohydrolase activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosyl-AMP cyclohydrolase"
                     /protein_id="HRV99737.1"
                     /translation="MNIELNSVKFDEQGLIPVIVQDVLSGEVLMMAWANRESLELTAS
                     TGDMTFWSRSRKELWRKGATSGNTMKVLELRADCDGDALLALVEASGPACHTGERSCF
                     FNVLTAPCEGRGTFPGVLWRYLEHRRFDDPSESYTARLLSR"
     gene            complement(419..1201)
                     /gene="hisF"
                     /locus_tag="P5201_14160"
     CDS             complement(419..1201)
                     /gene="hisF"
                     /locus_tag="P5201_14160"
                     /EC_number="4.3.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013048079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000107 - imidazoleglycerol-phosphate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="imidazole glycerol phosphate synthase subunit
                     HisF"
                     /protein_id="HRV99738.1"
                     /translation="MLTRRIIPCLDVLGGRVVKGVNFINLRDAGDPAELARTYMEEGA
                     DELVFLDITASAERRRTMLEWVSRVADTLFLPFTVGGGISTVEQAREIVALGADKISL
                     NTAAVRNPQLIADCARLLGRQAVVLAVDAKETPSGGWEVFVDGGRTATGLDVLEWIRE
                     GARLGCGEILLTSMDRDGAKNGYDNRLLSRAASSVSVPLIASGGAGSMEHILETFRAG
                     SDAALAASIFHYGEIRIGDLKRYLAGQGIPVRLTGSVASNEH"
BASE COUNT          221 a          449 c          413 g          233 t
ORIGIN      
        1 ccgcgagagg agccgcgccg tgtagctttc ggatggatcg tcgaaacggc ggtgctccag
       61 gtagcgccag agaaccccgg gaaacgtccc tctcccctcg cacggtgcgg tgagcacgtt
      121 gaaaaaacag gaacgctccc ccgtatggca ggcggggccc gacgcctcca cgagcgcgag
      181 cagcgcgtcg ccgtcgcagt cggcccgcag ttcgagcacc ttcatcgtgt tgccgcttgt
      241 cgctcccttc ctccacagct ccttccgcga tctgctccag aaggtcatat cgcccgtcga
      301 ggcggtcagt tccagagact cgcggttcgc ccacgccatc atgagaacct cccccgagag
      361 gacgtcctgg acgatgacgg ggatcagccc ctgttcgtcg aattttacgg aattcagctc
      421 aatgttcatt tgaagcaaca ctcccggtga gccggacggg gattccctgc ccggcaagat
      481 atcgtttgag atcgccgatg cgaatctccc cgtagtggaa gatggaggcg gcgagcgcgg
      541 cgtcgctccc tgcgcggaag gtctccagga tgtgctccat gcttcccgct ccccccgagg
      601 cgatcagcgg aacggagacg gaggaggcag ccctcgagag cagacggttg tcgtagccgt
      661 ttttcgcccc gtcgcggtcc atcgaggtca gcagaatctc gccgcacccg aggcgcgcgc
      721 cctccctgat ccattcgagt acgtccagtc cggttgcagt gcgtccgccg tcgacgaaga
      781 cctcccagcc tccggacggc gtttccttcg cgtccacggc gaggacgacc gcctgcctgc
      841 cgaggagcct ggcgcagtcg gcgatgagct gcgggttccg tacggcggcg gtgttcagcg
      901 aaatcttgtc cgcgccgagc gcgacgatct cccgggcctg ctccacagtg gagatgccgc
      961 ctccgacggt gaaggggagg aagagcgtgt ccgccacccg cgagacccat tcgagcatcg
     1021 tccgcctgcg ttcggcggag gccgtgatgt cgaggaagac cagttcgtcg gcgccctcct
     1081 ccatataggt gcgcgccagc tccgcagggt ctcccgcgtc gcgcaggttg atgaagttta
     1141 cgcccttcac cacgcgcccc ccgagcacgt cgaggcacgg gatgattcgc cgcgtcagca
     1201 tgaagcggcc tcccggagcg cgtccttcaa gaggatgctt ccttcgtaga gcgatctgcc
     1261 gagaacggcg cccgcgagcc cgtcctcctt cagatttcgt atgtcctcca gcgagg
//
LOCUS       DAOTIL010000880         1316 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminobacteriaceae bacterium isolate AMR_MDS_3026
            NODE_121791_length_1316_cov_2.210944, whole genome shotgun
            sequence.
ACCESSION   DAOTIL010000880 DAOTIL010000000
VERSION     DAOTIL010000880.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868050
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminobacteriaceae bacterium (wastewater metagenome)
  ORGANISM  Aminobacteriaceae bacterium
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae.
REFERENCE   1  (bases 1 to 1316)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1316)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:19:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,224
            CDSs (total)                      :: 3,198
            Genes (coding)                    :: 3,184
            CDSs (with protein)               :: 3,184
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1316
                     /organism="Aminobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_121791_length_1316_cov_2.210944"
                     /isolate="AMR_MDS_3026"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:3034609"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..324
                     /locus_tag="P5201_14165"
     CDS             <1..324
                     /locus_tag="P5201_14165"
                     /inference="COORDINATES: protein motif:HMM:NF023958.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine 2,3-aminomutase"
                     /protein_id="HRV99739.1"
                     /translation="SGLAVPTFVVDAPGGGGKIPVMPNYVISRSDRKTILRNYEGVIT
                     VYTEPDDNQSKCLGKCRDLCERAKATFSGGIQSLFEGNAVSIEPKELLREKRRKACED
                     SSKQE"
     gene            complement(412..861)
                     /locus_tag="P5201_14170"
     CDS             complement(412..861)
                     /locus_tag="P5201_14170"
                     /inference="COORDINATES: protein motif:HMM:NF015648.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PH domain-containing protein"
                     /protein_id="HRV99740.1"
                     /translation="MEQQETFEKRMRPAWRSFLTWWILSVAVAALAVFLSVRYTAPWW
                     TVAVALIPVVVIMLKRCSTEFIVKHDEVTLEKGLFAKNSVEVGMPQIRTIEVRQTVFQ
                     RMLGIGDIMIASSGTDTYEIIVSGIEEPKELRDVIQGFQRRAASAAE"
BASE COUNT          374 a          369 c          295 g          278 t
ORIGIN      
        1 tccgggctgg cagtccccac gttcgttgtg gacgcgccgg gaggaggcgg caaaattccg
       61 gtcatgccta actatgtgat ctcgcgctcg gacagaaaga cgatactgcg caactacgag
      121 ggagtcatta ccgtatatac ggagccggac gataatcaga gcaagtgcct cgggaagtgc
      181 cgcgatctct gtgagcgtgc gaaagctacc ttttccggag gcatccagtc tcttttcgaa
      241 ggcaacgccg tctcgatcga gccgaaagaa ctgctcaggg agaaacgtcg aaaggcatgc
      301 gaagattcct caaaacaaga ataaaaaaga aagaagaggg gagttcacca cgcccctctt
      361 ctttgtacgt atcttgcatg tttaaaaaaa gcgtattctg cggcgaaaag actattccgc
      421 tgcgctcgcg gctctcctct gaaaaccctg aatgacatcg cgaagctctt tgggctcctc
      481 tatgccggaa acaataattt catacgtgtc cgttcctgag gaagcgatca tgatatcgcc
      541 tataccaagc attctctgaa aaaccgtctg ccggacttca atggttcgga tctgcggcat
      601 accgacttca acagaattct ttgcaaacag ccccttctcc agagttacct cgtcatgctt
      661 cacgatgaac tccgtcgaac agcgcttcag catgatgaca acaacaggaa tcaatgccac
      721 tgcgactgtc caccacggcg ctgtgtaacg cacactgaga aaaacagcga gcgcggccac
      781 agcaacactc agtatccacc acgtcaaaaa acttctccat gctggacgca ttctcttctc
      841 gaacgtctct tgctgctcca taaataaacc cctcccgtca caaattttat agcttcttcg
      901 aaatcaaccg ataaacacgc gccaacgggg taccgccatt cagagcatgg atgcttatta
      961 tctcatacgt gaaggctcgt tttcccgaaa cgactctgcg ctccgaaaaa aacatttccg
     1021 acgcgctgta aggacgacgc atcggaaaca ttttacacca ccttggcatt ccatggaaga
     1081 agaaagcagc aaaagtacgc aaaaaaacga aacgagcaag aacaaacttc agatgcgagc
     1141 gtttctcctc cacttacctc aacagaaaaa tactattccg ccatttcccg gaattgtttt
     1201 gtatagagca acggcaacgc aataagcgga agaaaggcca ccacgagaag cgtcgtctgt
     1261 acaccgaaga ggtcgccgat cttccccagc ggcgccatca gcatgttgcc gaatcc
//
LOCUS       DAOTIL010000881         1314 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminobacteriaceae bacterium isolate AMR_MDS_3026
            NODE_122042_length_1314_cov_2.490866, whole genome shotgun
            sequence.
ACCESSION   DAOTIL010000881 DAOTIL010000000
VERSION     DAOTIL010000881.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868050
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminobacteriaceae bacterium (wastewater metagenome)
  ORGANISM  Aminobacteriaceae bacterium
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae.
REFERENCE   1  (bases 1 to 1314)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1314)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:19:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,224
            CDSs (total)                      :: 3,198
            Genes (coding)                    :: 3,184
            CDSs (with protein)               :: 3,184
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1314
                     /organism="Aminobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_122042_length_1314_cov_2.490866"
                     /isolate="AMR_MDS_3026"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:3034609"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..304)
                     /locus_tag="P5201_14175"
     CDS             complement(<1..304)
                     /locus_tag="P5201_14175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005658641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine reductase"
                     /protein_id="HRV99741.1"
                     /translation="MSEIKKLIGEALADIVKEARTGGPRVSVGLMALGSELGGEELAR
                     GALLASENSSLIEVVMIGPRIPGFEKLSWIETEPCEENVAAAMEKALAEGRISGAVA"
     gene            complement(320..>1314)
                     /gene="grdC"
                     /locus_tag="P5201_14180"
     CDS             complement(320..>1314)
                     /gene="grdC"
                     /locus_tag="P5201_14180"
                     /EC_number="1.21.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013048772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="glycine/sarcosine/betaine reductase complex
                     component C subunit beta"
                     /protein_id="HRV99742.1"
                     /translation="DCLFAYVLLENLACKAGGVLSLLHLLKKTGMTPEEVDFVVECSE
                     EGAGDMNQRAGGNFAKAIAEIAGCANASGCDVRSFCAGPVNAMIAAASQVACGARKNC
                     VVLAGGAVPKLFMNGRDHVKKKMPALEDCMGNFAILLVPDDGELPVMRLDVLGKHTVA
                     AGSSPQAVTTALILDPLERAGLSFLDVDKFAAEMHINEITLPAGAGDVPLANIKMTAA
                     LAVTKKAIEKADMMTFVKERGVPGIAHTQGHIPSGVPFVGHACDAMVAGKMKRAMIIG
                     KGSLFLARLTNLADGASFLLEPPTGKKSSVAAVSKDDVKALLLEVLSELSGKLA"
BASE COUNT          256 a          407 c          372 g          279 t
ORIGIN      
        1 gtgcgacggc gccggaaatg cgcccctcgg cgagcgcctt ttccatagct gcggcaacat
       61 tctcttcaca cggctcggtt tctatccagg agagcttttc aaaaccgggt atccgcggcc
      121 cgatcatcac gacttcaatg agtgaggaat tctcggaagc gagtagtgcg ccgcgcgcca
      181 gttcttctcc gccaagttcg ctgcccagcg ccatgagccc cacggagacg cggggtccgc
      241 ccgtacgggc ctccttcaca atatccgcca gggcctctcc gatgagtttt ttaatctcgg
      301 acatctcggc caccaccggc tatgcgagct tcccggacag ttcggagaga acctcgagga
      361 gaagcgcttt tacatcgtct ttcgagactg ctgcaaccga gctcttctta cccgtcggag
      421 gttcgagcag gaaagaagcg ccgtcggcga ggtttgtcaa acgagcgagg aagaggcttc
      481 ccttgccgat gatcatcgct ctcttcatct tccctgcgac catcgcgtcg caggcatgtc
      541 cgacaaacgg aactcctgaa ggaatatgtc cttgagtatg cgcgattccc ggtacgccgc
      601 gctccttgac gaaggtcatc atatcggcct tctcaatggc cttttttgtc accgccagcg
      661 cggcggtcat cttgatgttc gcaagcggaa cgtcgcctgc gcctgcaggg agagtgatct
      721 cgttgatatg catctcggcc gcgaacttgt ccacgtcgag gaaggaaagt cccgccctct
      781 caagcggatc gaggatgagc gcagtggtaa ctgcctgagg agaggaacct gcggcgaccg
      841 tatgtttccc gagaacgtcg agacgcatca ccggaagttc tccgtcatcc gggacaagga
      901 gtatcgcaaa gttgcccatg cagtcctcaa gcgcgggcat cttctttttc acgtggtcgc
      961 gcccgttcat gaagagcttc gggacagccc ctcctgcgag gacgacacag ttcttccgag
     1021 cgccgcaggc gacctgggac gcggcggcga tcatcgcgtt caccggtcct gcgcagaaac
     1081 tgcggacatc gcatccgctg gcgttcgcgc atcccgcgat ttccgcgatc gccttggcga
     1141 agttgcctcc ggctctctgg ttcatatctc ctgcgccctc ttcggagcat tcgacgacaa
     1201 agtcgacttc ctcgggagtc attccggtct tcttgaggag atggagcagg gagagcaccc
     1261 ctcccgcttt gcaggcaaga ttctcaagaa gcacataggc gaagagacag tcgt
//
LOCUS       DAOTIL010000882         1314 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminobacteriaceae bacterium isolate AMR_MDS_3026
            NODE_122052_length_1314_cov_2.321684, whole genome shotgun
            sequence.
ACCESSION   DAOTIL010000882 DAOTIL010000000
VERSION     DAOTIL010000882.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868050
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminobacteriaceae bacterium (wastewater metagenome)
  ORGANISM  Aminobacteriaceae bacterium
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae.
REFERENCE   1  (bases 1 to 1314)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1314)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:19:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,224
            CDSs (total)                      :: 3,198
            Genes (coding)                    :: 3,184
            CDSs (with protein)               :: 3,184
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1314
                     /organism="Aminobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_122052_length_1314_cov_2.321684"
                     /isolate="AMR_MDS_3026"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:3034609"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..758
                     /locus_tag="P5201_14185"
     CDS             <1..758
                     /locus_tag="P5201_14185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012869124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="TAXI family TRAP transporter solute-binding
                     subunit"
                     /protein_id="HRV99743.1"
                     /translation="RGEAEMAIAMANITGFAYTGTQTYEGKKIENLRYVMGLWPDVTQ
                     FVVSGSSGIKTWADLKGKKVAVGPAASGTEFSSKVLLQALAGLTFDDIKAEYVGYSEA
                     AQALQNGQLDAFNAEAGVPVGAVAELYAGRQEVNMLEFSAEDLAKVKAVAPFYAGVLI
                     PAGTYPKQEKDLRVAGIKSALLVGKDVPDEVVYDMLKIIYAKKDDLLNEHAAFKKVDF
                     DNPIDGLSGAPLHPGAVKFFSEIGLTIPAELLP"
     gene            841..>1314
                     /locus_tag="P5201_14190"
     CDS             841..>1314
                     /locus_tag="P5201_14190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014162667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter permease"
                     /protein_id="HRV99744.1"
                     /translation="MFMKGFVSLFVPGESIPTRKLTGPAGAAALVLTVATALVHFWMN
                     SIGLLIAVKMNAIHLGTLMAIIFLFYPAFAGSPRERPSLPDWVLALTSLGCMFWLLFT
                     YDRLLQTNLQATFADLAVAVVTMTLLVEASRRAVGLPLTLLSIFFLAYTRFGPMFP"
BASE COUNT          239 a          384 c          385 g          306 t
ORIGIN      
        1 agcgcggaga ggccgagatg gcgatcgcca tggcgaacat caccggcttc gcctacaccg
       61 ggacgcagac ctacgaaggg aagaagatcg aaaacctccg gtatgtcatg gggctgtggc
      121 cggacgtgac gcagtttgtc gtcagcggct cctccggcat taaaacctgg gccgatctca
      181 agggcaagaa ggtcgccgtg ggacctgccg cctccggcac ggagttcagc agcaaggtgc
      241 ttcttcaggc ccttgccggt ttgaccttcg acgatatcaa ggccgagtac gtgggataca
      301 gcgaggcagc ccaggcgctt cagaacgggc agcttgacgc gttcaacgcc gaggccggcg
      361 tccctgtagg ggcggtcgcc gaactctatg cgggccgcca ggaagtcaac atgcttgagt
      421 tctctgcgga ggatctggcg aaggtaaagg cagtcgctcc cttctatgca ggcgtgctta
      481 tcccggccgg aacctatccg aagcaggaga aggatctccg ggtcgccggc atcaagtcgg
      541 cgctgcttgt cggaaaggac gttcccgatg aagtcgtcta cgacatgctg aagatcatct
      601 acgcgaaaaa ggatgatctt ttgaatgagc atgccgcctt caaaaaggtc gattttgata
      661 acccgatcga cggtctttca ggggcgcctc tgcatccggg cgcggtgaaa ttcttctcgg
      721 agatcggtct gaccattccg gcggagcttc tcccgtagtc tcttttccgt ctgttcttta
      781 ttcgacgcgg gcggcgtggg gcgatcccat gccgctcctt tttcaggagg agcgtatcgt
      841 atgtttatga aaggttttgt ttcccttttc gttccgggcg agtccattcc aacccgtaag
      901 ctcaccggtc ctgccggagc cgccgcgctt gtgctcaccg tcgcgacggc tctcgtccat
      961 ttctggatga acagcatcgg cctgctgatc gcagtgaaga tgaacgccat tcacctgggg
     1021 acgctcatgg cgatcatctt cctcttttac ccggccttcg cgggttcccc aagagaacgc
     1081 ccgtctcttc ccgactgggt tctggcgctg acatccctcg gatgcatgtt ttggttgctc
     1141 ttcacctacg acaggttgct tcagacgaac ctgcaggcga cgttcgcgga tcttgccgtt
     1201 gcagtggtta caatgacgct tcttgtagag gccagccgcc gggctgtagg cttgccgttg
     1261 acgctactca gtattttctt tctcgcctac acccgtttcg gtcccatgtt tccg
//
LOCUS       DAOTIL010000883         1313 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminobacteriaceae bacterium isolate AMR_MDS_3026
            NODE_122134_length_1313_cov_3.717806, whole genome shotgun
            sequence.
ACCESSION   DAOTIL010000883 DAOTIL010000000
VERSION     DAOTIL010000883.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868050
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminobacteriaceae bacterium (wastewater metagenome)
  ORGANISM  Aminobacteriaceae bacterium
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae.
REFERENCE   1  (bases 1 to 1313)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1313)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:19:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,224
            CDSs (total)                      :: 3,198
            Genes (coding)                    :: 3,184
            CDSs (with protein)               :: 3,184
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1313
                     /organism="Aminobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_122134_length_1313_cov_3.717806"
                     /isolate="AMR_MDS_3026"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:3034609"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..476
                     /locus_tag="P5201_14195"
     CDS             <1..476
                     /locus_tag="P5201_14195"
                     /inference="COORDINATES: protein motif:HMM:NF015129.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit beta"
                     /protein_id="HRV99745.1"
                     /translation="FVQGEEHFGHRGQTALLYCGEKAVGYLLRLKPSIEKELDCAPLF
                     AFELDLEPFETSALPEFREPSSFPPVYRDISLFVPLAESMERVAEHIREEGGSLLRDV
                     RLFDMYSGKGVPEGFRGLAFSLAYKRDDRTLTDKEVDEVNERVRSRMSSSGYVLR"
     gene            489..812
                     /locus_tag="P5201_14200"
     CDS             489..812
                     /locus_tag="P5201_14200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006583684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRV99746.1"
                     /translation="MTTIGNIEHLVDKLCATVSSLRTERDQLRAQVDELKSKFAEKDL
                     ELIRTGKENQRTLEVLEREKLTLQKEKNQIEGQLKAIYDRISTLMPELSGTLSGAGGE
                     RSARG"
     gene            809..1051
                     /locus_tag="P5201_14205"
     CDS             809..1051
                     /locus_tag="P5201_14205"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRV99747.1"
                     /translation="MTSSLRDVTIKVGRSVYHVRTSLDDESLLRVTTLMAEFTGELEH
                     TLDQEKILLLLCLQLGWTLEKIRSKIDGISEELEEQ"
     gene            1048..>1313
                     /locus_tag="P5201_14210"
     CDS             1048..>1313
                     /locus_tag="P5201_14210"
                     /inference="COORDINATES: protein motif:HMM:NF014704.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CvpA family protein"
                     /protein_id="HRV99748.1"
                     /translation="MNVASVFDIVMLLIVITLVLRGLFRGFSGEFFSLLGTVGGIILA
                     WKYSDPLAEKLAEIWPQGGSALLSVSAMALIYIAAVVGAALMCK"
BASE COUNT          288 a          347 c          406 g          272 t
ORIGIN      
        1 gtttcgtcca gggagaggag cacttcggcc acagggggca gaccgcgctc ctgtactgcg
       61 gagagaaggc cgtgggatat ctgcttcgcc tcaagccgtc gatcgaaaag gagcttgact
      121 gcgctccgct ctttgctttt gaactggatc tggagccgtt tgaaacgtct gcgctccctg
      181 aattcagaga accttcttct ttccctccgg tctacaggga tatctccctc ttcgtcccgc
      241 tggcggagtc gatggagcgg gtggcggagc acatccggga ggaaggaggc tcactcctcc
      301 gcgacgtgcg cctcttcgac atgtactcag gcaaaggagt tcccgaaggc ttccgggggc
      361 tggcgttctc tctcgcatac aaacgggatg acaggacgct caccgacaag gaagtggacg
      421 aagtcaacga gcgagttcgc agcagaatga gctcgtccgg atacgtcctt cgataaggag
      481 ggggctgaat gacgacgatc ggcaacatcg agcatctggt cgacaagctc tgcgcgaccg
      541 tgtcgtcgct gcggaccgag cgcgaccagc ttcgcgccca ggtcgacgag ctcaaaagca
      601 aatttgccga gaaggatctc gaactgatca ggacggggaa ggaaaatcag cggacgctgg
      661 aggtgctcga aagggagaag cttaccctcc agaaggagaa gaaccagata gaaggtcagt
      721 tgaaggccat ctatgatcgt atttcaaccc tcatgcccga gttgtcgggc acactatccg
      781 gcgccggagg ggaaaggagc gccagagggt gacgtcatcc cttcgcgatg taacaatcaa
      841 ggtcggcagg agcgtctacc acgtcaggac atctctcgac gacgagagcc tgctgagggt
      901 aacaacgctc atggccgagt tcaccggcga gctggagcac acgcttgatc aggagaagat
      961 tcttcttctc ctgtgtctcc agctcggctg gacccttgag aagatacgct ccaaaataga
     1021 cggaatttcg gaggagcttg aagaacaatg aatgtggctt cagtttttga tatcgtaatg
     1081 ctgttgatag taatcaccct ggtgcttcgg gggctcttca ggggcttttc cggagagttt
     1141 ttctctttgc tggggactgt gggaggcatc atcctcgcct ggaaatactc cgatccgctt
     1201 gcagagaagc tggcggagat ctggccccag ggagggagcg cgctgctctc ggtctcggcc
     1261 atggcgctaa tctatatcgc ggcggtcgtc ggcgcggcgc tcatgtgtaa aat
//
LOCUS       DAOTIL010000884         1313 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminobacteriaceae bacterium isolate AMR_MDS_3026
            NODE_122147_length_1313_cov_3.354531, whole genome shotgun
            sequence.
ACCESSION   DAOTIL010000884 DAOTIL010000000
VERSION     DAOTIL010000884.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868050
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminobacteriaceae bacterium (wastewater metagenome)
  ORGANISM  Aminobacteriaceae bacterium
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae.
REFERENCE   1  (bases 1 to 1313)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1313)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:19:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,224
            CDSs (total)                      :: 3,198
            Genes (coding)                    :: 3,184
            CDSs (with protein)               :: 3,184
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1313
                     /organism="Aminobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_122147_length_1313_cov_3.354531"
                     /isolate="AMR_MDS_3026"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:3034609"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..963)
                     /locus_tag="P5201_14215"
     CDS             complement(<1..963)
                     /locus_tag="P5201_14215"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRV99749.1"
                     /translation="MRTDTNAHTPPAATLLSPEAFLYSASIPGFFSALPLDERSSLSV
                     ATGRSALAKALDTASQKALTGRAWLPSLCCASLAPAFLQRGFSISFYSTAPFIPPRLE
                     RGDVFLYIHFCGFPNRIAERALESLPEERRPIVVEDCVHALFTRGTGRFGDFALYSFR
                     KFLPVPDGGLLLSREPLDDTLSPPLERFISLKTAGLLSESRRLLEEGEEALDADDEAR
                     EPSVMGALLLDRLPWTHIPEARRRNYAFLAERLRLPPLDDGTVPLGAPLRISAGKAEL
                     ERRLRASGFEPPLSWDAPRDAPSDEADRLVVLPCDERMGERELAR"
     gene            complement(977..>1313)
                     /locus_tag="P5201_14220"
     CDS             complement(977..>1313)
                     /locus_tag="P5201_14220"
                     /inference="COORDINATES: protein motif:HMM:NF012692.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HRV99750.1"
                     /translation="GADALERTAKSMGLQPDLKYLWTRRTDPAVAPLWDRGLDHLAKG
                     IASAIHLFDPQAVILGGGMSRGEGFIDVLKPRVVDYLALPFRKTLDLRPSLLGNDAPT
                     IGAAALTRK"
BASE COUNT          241 a          395 c          475 g          202 t
ORIGIN      
        1 ccgcgcaagc tcccgctccc ccatcctctc gtcgcacggc aaaacgacga gcctgtcggc
       61 ctcgtccgag ggagcgtcgc ggggagcgtc ccatgagaga ggcggctcga agcccgacgc
      121 ccgaaggcgg cgttcgagtt cggcttttcc cgcggatatg cggagcggag ccccgagcgg
      181 aaccgtgccg tcgtcgagcg gcggcaggcg caggcgttcc gcgaggaagg cgtagttccg
      241 tctgcgagct tcgggaatgt gcgtccacgg gagccggtcg agaaggagcg ctcccataac
      301 agaaggctct ctggcctcgt cgtccgcgtc gagcgcctcc tctccctctt cgagaaggcg
      361 gcgcgattcc gacagaagcc ccgccgtctt gagggagatg aagcgttcga gcggcggaga
      421 gagcgtgtcg tcgagcggct cgcgggagag gagcagccca ccgtccggga cggggagaaa
      481 cttgcggaag ctgtagagcg cgaagtcgcc gaagcgtcct gttcctcgtg tgaagagggc
      541 gtggacgcag tcctcgacga cgatcgggcg gcgctcctcc ggcagggatt ccaacgcccg
      601 ctcggcaatc ctgttcggga agccgcagaa gtggatgtac aggaagacgt cccctcgttc
      661 aagcctgggg gggataaacg gcgccgtact gtagaaggag atggagaacc ctctctgcaa
      721 aaaagcgggg gcgagcgacg cgcagcagag cgaagggagc cacgccctcc ccgtaagcgc
      781 tttttgggaa gcggtgtcga gcgcctttgc aagcgctgat cttcctgtgg cgacggagag
      841 cgaggagcgt tcgtcgagcg ggagggcgga gaaaaaaccg gggatggagg cggagtacaa
      901 aaaagcctcc ggagacagca acgtggccgc cggaggcgta tgagcgtttg tatcggtgcg
      961 catgtctgcg aggcgcttac ttccgagtga gggccgcggc gccgatggtg ggcgcgtcgt
     1021 tgccgagcag gctcgggcgc agatcaagcg tcttgcggaa agggagcgca aggtagtcca
     1081 ctactcttgg cttcaggacg tcgataaacc cctcgccgcg gctcatgccg ccgccgagga
     1141 tgaccgcctg cgggtcgaag aggtgaatcg ccgaggcgat ccccttcgcc aagtggtcca
     1201 gccctcggtc ccacagaggc gctacagccg ggtcggtcct gcgggtccag aggtacttca
     1261 ggtcgggctg caaacccatc gacttcgctg tgcgctccag ggcgtccgcg ccg
//
LOCUS       DAOTIL010000885         1312 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminobacteriaceae bacterium isolate AMR_MDS_3026
            NODE_122254_length_1312_cov_4.188544, whole genome shotgun
            sequence.
ACCESSION   DAOTIL010000885 DAOTIL010000000
VERSION     DAOTIL010000885.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868050
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminobacteriaceae bacterium (wastewater metagenome)
  ORGANISM  Aminobacteriaceae bacterium
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae.
REFERENCE   1  (bases 1 to 1312)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1312)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:19:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,224
            CDSs (total)                      :: 3,198
            Genes (coding)                    :: 3,184
            CDSs (with protein)               :: 3,184
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1312
                     /organism="Aminobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_122254_length_1312_cov_4.188544"
                     /isolate="AMR_MDS_3026"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:3034609"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..751)
                     /locus_tag="P5201_14225"
     CDS             complement(<1..751)
                     /locus_tag="P5201_14225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005659336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent dehydrogenase
                     E1 component subunit alpha"
                     /protein_id="HRV99751.1"
                     /translation="MYAQPVRKEATLSPAKYEKDLLGYFYKTMVTIRSFEQMIEKFFL
                     EGEIPGFVHLYIGEEAIATGVMANLRKTDYIQSTHRGHGHTIAKGADLKRMMAELFGK
                     STGSCKGKGGSMHIADFSVGMLGANGIVGGGFTMATGAALAHKMQKTDGVAVTFFGDG
                     ASNRGTFHEAANMAAAWNLPVIFVCENNQWASTTPYRTTTSVDDIADRAVAYGMPGVI
                     VDGNDVFSVCEAAKELVERARRGEGPSILECK"
     gene            complement(913..>1312)
                     /locus_tag="P5201_14230"
     CDS             complement(913..>1312)
                     /locus_tag="P5201_14230"
                     /inference="COORDINATES: protein motif:HMM:NF019349.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="FCD domain-containing protein"
                     /protein_id="HRV99752.1"
                     /translation="EEFVIKEIIPFMTEQRTAEMDRNLALQKEAVDKGDVSEYLRHDK
                     EFHDHFLRIYSNALIFKTVQRVRDRFISMGANVLREPGSVERSFAEHCSIADAVRRSD
                     RGGAAEAMHRHLITGRNNVLSMPGNLGAGI"
BASE COUNT          252 a          417 c          346 g          297 t
ORIGIN      
        1 atttgcactc cagaatagaa ggcccctccc cccggcgcgc tctttcgacg agctccttcg
       61 ccgcctcgca gacagagaaa acatcgttcc cgtccacaat aacgcccggc attccataag
      121 cgacggcccg gtcggcgatg tcgtccacag aagtcgtggt gcgatacgga gtcgttgaag
      181 cccactggtt gttctcgcag acgaagatca ccggcaaatt ccacgccgcg gccatattcg
      241 ccgcctcgtg gaaagttccc cggttggatg cgccgtcgcc gaagaaagtg accgcgacgc
      301 cgtcggtctt ctgcattttg tgcgccaacg cggcgcctgt cgccattgtg aatccgccgc
      361 cgacgatgcc gttcgcgccg agcatcccca cgctgaagtc ggcgatgtgc atcgaaccgc
      421 cctttccctt gcagcttccg gtgctcttgc cgaagagctc cgccatcatc cgtttgagat
      481 ccgctccctt ggcgatggtg tggccgtgcc cgcggtgcgt gctctggata taatcggtct
      541 ttcgcaagtt ggccatgacg cctgtcgcga ttgcctcctc gccgatatac aggtggacga
      601 atcccggaat ctcgccttca agaaagaact tctcgatcat ctgctcgaaa ctcctgatcg
      661 tcaccattgt cttgtagaaa tagcccaaca gatctttttc atattttgcg ggagaaagag
      721 tcgcctcttt ccgcacaggc tgcgcataca tacaaatcac ctcgttgaaa gtctggtctt
      781 tttccgaata cggcccttta cgcggccctc ttcttcttgt tcatgcgcgg ttcttcgaaa
      841 aagcgccccc ccttcctcga atccgccgcg gcctctcccg gtcacgaaga acggggcggc
      901 cgcgtagagc tctcaaatcc ccgcgccgag atttcccggc atagagagaa cattgtttct
      961 gcctgtaatc agatgacggt gcatagcctc ggcagcgccg cccctgtcgc tcctgcgaac
     1021 ggcgtcggcg atactgcaat gctccgcgaa ggatcgttcg acactccccg gctcgcggag
     1081 gacgttggcc cccatggaga tgaaacggtc cctgacacgc tgaactgtct tgaagataag
     1141 agcgttggaa taaatacgga ggaaatggtc gtgaaactct ttgtcgtggc ggaggtactc
     1201 cgagacgtcg cccttgtcga cggcctcttt ctgaagcgcg agattgcggt ccatctccgc
     1261 cgtccgctgt tccgtcataa aggggatgat ctctttgatc acgaactcct cg
//
LOCUS       DAOTIL010000886         1312 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminobacteriaceae bacterium isolate AMR_MDS_3026
            NODE_122299_length_1312_cov_2.762132, whole genome shotgun
            sequence.
ACCESSION   DAOTIL010000886 DAOTIL010000000
VERSION     DAOTIL010000886.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868050
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminobacteriaceae bacterium (wastewater metagenome)
  ORGANISM  Aminobacteriaceae bacterium
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae.
REFERENCE   1  (bases 1 to 1312)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1312)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:19:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,224
            CDSs (total)                      :: 3,198
            Genes (coding)                    :: 3,184
            CDSs (with protein)               :: 3,184
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1312
                     /organism="Aminobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_122299_length_1312_cov_2.762132"
                     /isolate="AMR_MDS_3026"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:3034609"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..306)
                     /locus_tag="P5201_14235"
     CDS             complement(<1..306)
                     /locus_tag="P5201_14235"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRV99753.1"
                     /translation="MSNTQCLVVVDYQKDFVDGVLGCGQPALDIESFIEKKIQEHLDA
                     RHTVFFTLDTHTEPVYSDSVESSSYTLHCRKYTEGWLPYGVIKNYMTYPGVEVIEKAT
                     "
     gene            complement(438..584)
                     /locus_tag="P5201_14240"
     CDS             complement(438..584)
                     /locus_tag="P5201_14240"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRV99754.1"
                     /translation="MQKRWIGYMTAEDAEFDTAWTRLDDLYASFVQQDGEYPLHDLLG
                     VKRV"
     gene            complement(587..1078)
                     /locus_tag="P5201_14245"
     CDS             complement(587..1078)
                     /locus_tag="P5201_14245"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRV99755.1"
                     /translation="MSRTYAEITAVVLFLSACIILPVSWWLSGETNAFRERGVPTMVR
                     IIRFEEVASGEDGTRRIAVAERVTDGRRPAGEDLLASHILMDEITEVGSLHLGQELEV
                     AVLPDAEFFMTPPGYTHWFITRRSLDRHTRQPISEWGWNSALVLTALSLLLGAYSASK
                     ERK"
BASE COUNT          267 a          388 c          344 g          313 t
ORIGIN      
        1 ggttgccttt tcgatcactt ccacgccggg gtacgtcata tagtttttga tcacgccgta
       61 gggaagccag ccctccgtgt atttccggca gtgcagcgta tatgaggagc tttcgacgga
      121 atccgaatag acgggctccg tgtgggtgtc cagcgtgaag aaaactgtat gacgcgcgtc
      181 gagatgctct tgaatcttct tctcgatgaa actctcgata tccagcgccg gctgtccgca
      241 gccaaggacg ccgtccacaa aatccttctg gtaatcgaca acgacgaggc actgcgtgtt
      301 cgacattcaa attcccaccc ttccgtggtg tagaagacac tttgaaaaag cataccgtat
      361 gctcctttat ctattcttgt ttttcccttg attcgacgca ggacgcggtt tttcgcggtg
      421 tttccatgcc ctcttcttca caccctcttc acgccgagga ggtcgtggag cggatactcg
      481 ccgtcctgct ggacgaagga tgcgtacagg tcgtcgagcc gcgtccatgc cgtgtcgaat
      541 tccgcgtcct ccgccgtcat gtagccgatc caccgtttct gcatgattat ttcctctcct
      601 tcgaagccga gtacgccccc agaagcagcg aaagcgccgt tagaacaagc gcggagttcc
      661 agccccattc ggagatgggt tgccgcgtat gccggtccag cgaacggcgc gtgatgaacc
      721 agtgggtata gcccggaggc gtcatgaaaa actccgcatc gggcaggacg gccacttcca
      781 actcctgccc caagtgaagc gagcccacct ccgttatctc gtccatcaga atgtgggacg
      841 ccagaagatc ctccccggca ggcctccgtc cgtccgttac tctctcggcg acggctattc
      901 tccgtgttcc atcctccccg ctggcaacct cctcgaagcg gataatgcgg accatcgtcg
      961 gtactccgcg ctccctgaag gcgttcgtct ccccggagag ccaccaggag accggcaata
     1021 tgatacacgc ggatagaaag aggacgaccg cagtgatctc cgcatacgtt ctgctcatgt
     1081 ttctcctccg tatcgtcgcc tgggatgcct tctttgtgta ttgtatcact tatatttccg
     1141 acctcgggaa acgaacctga ggatgtgagt gagaggagtt tcgacacgcg aagcgcaact
     1201 ttatgttaga atttatcggg gctcaatgcc ctgtttcaga ttgcgaaatt acgttacagg
     1261 aggtcctacc ctcgcggatg tgggcgccag cgtcgactcg aaagcatttg ta
//
LOCUS       DAOTIL010000887         1311 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminobacteriaceae bacterium isolate AMR_MDS_3026
            NODE_122440_length_1311_cov_3.215764, whole genome shotgun
            sequence.
ACCESSION   DAOTIL010000887 DAOTIL010000000
VERSION     DAOTIL010000887.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868050
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminobacteriaceae bacterium (wastewater metagenome)
  ORGANISM  Aminobacteriaceae bacterium
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae.
REFERENCE   1  (bases 1 to 1311)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1311)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:19:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,224
            CDSs (total)                      :: 3,198
            Genes (coding)                    :: 3,184
            CDSs (with protein)               :: 3,184
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1311
                     /organism="Aminobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_122440_length_1311_cov_3.215764"
                     /isolate="AMR_MDS_3026"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:3034609"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..729)
                     /locus_tag="P5201_14250"
     CDS             complement(<1..729)
                     /locus_tag="P5201_14250"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRV99756.1"
                     /translation="MNTCSEVFIITHAGRSIGGGHASRCSALAEGFSSLGVAVRWLVN
                     ASAGEIVLRRGATEASVTVMESPFGDGADAVFAAISRSRPALCVVDGYDATPSFLREL
                     RRFAPVVLVDDCRVRPVERECDVVLNYNMNADLLGYEQGHAGLLLGSEYALLRSEFWT
                     LAPEKGDVVLIIPGASDLLHTGERFVEWWGNGWPRAELVLGPLVESSMAERLSEAAGA
                     LPNLSAVRNPLDLPARMARSRAVLC"
BASE COUNT          312 a          426 c          341 g          232 t
ORIGIN      
        1 gcacagcacc gcgcgcgacc tcgccatccg ggcaggaagg tcaagcgggt ttcgcactgc
       61 cgaaaggttc ggcaacgcgc cggcagcctc ggacagtcgc tccgccattg aagactccac
      121 cagcggaccg aggacaagct ccgcacgcgg ccagccgttt ccccaccact ccacgaaccg
      181 ttcgcccgta tgcagcaaat cgctcgcgcc gggaatgatc agcacaacat cgcccttctc
      241 aggcgccaga gtccagaact cgctccgcaa cagggcgtac tcggacccca gaagcagccc
      301 ggcatgcccc tgttcgtatc cgagcaggtc cgcattcata ttgtagttca gcaccacgtc
      361 gcattcgcgc tcgacgggcc gtacccggca gtcgtccacc agcaccaccg gggcgaaccg
      421 gcgtagctcc ctcagaaaag aaggagttgc gtcgtacccg tccaccacgc agagggctgg
      481 acgagaacgt gagatcgcag caaacacagc atccgccccg tcgccgaacg gactctccat
      541 caccgtcacg gacgcttcgg tagcccctct gcgcagcaca atctccccgg cggaagcgtt
      601 caccagccaa cggacagcaa cgccgagcga agagaacccc tcggccagcg ccgagcagcg
      661 gctcgcatgc ccgcccccga tggaccgccc cgcgtgggta attatgaaaa cttcgctgca
      721 agtattcatg tgaccgactc cgtttactct gattttgccg ctgacagcgc ttccgcattc
      781 gcatcgcgct gtgcttttat tatacctaca gtggaaaatc ttgttgttga gcgttgtcga
      841 ggaaagaacg caggagagag gggagacgtt aggtgagaag agagacgaga agaagcaaaa
      901 atttaccgtt gtcgctaaga tgctgtgcaa aggtaaccct cacgaaaaga ttctggtcat
      961 agggagatat ctccggagga actcgacgag atggtcgaat cgcgcaaaaa ctgagcggca
     1021 ccctcacccc tcgtcactct ctccacatcc tcccggcgaa gacggctcac caatcgatac
     1081 agttttttgc tccttgacat caacagacaa tccaaagacc tctttgttag caagtggctc
     1141 tgcttgatta taacactcga cggcttctcg ccagggaata cagcaaggct taaaatgaca
     1201 cgcgaaaggc acgtcttaaa aattcggcgt tatgccgtca cgtaatcaaa agcacggctt
     1261 ccagacacat tctccgcacg cgacggtcgt gctcagctcg cccggcgcct c
//
LOCUS       DAOTIL010000888         1307 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminobacteriaceae bacterium isolate AMR_MDS_3026
            NODE_123009_length_1307_cov_3.876198, whole genome shotgun
            sequence.
ACCESSION   DAOTIL010000888 DAOTIL010000000
VERSION     DAOTIL010000888.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868050
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminobacteriaceae bacterium (wastewater metagenome)
  ORGANISM  Aminobacteriaceae bacterium
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae.
REFERENCE   1  (bases 1 to 1307)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1307)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:19:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,224
            CDSs (total)                      :: 3,198
            Genes (coding)                    :: 3,184
            CDSs (with protein)               :: 3,184
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1307
                     /organism="Aminobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_123009_length_1307_cov_3.876198"
                     /isolate="AMR_MDS_3026"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:3034609"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>1307
                     /locus_tag="P5201_14255"
     CDS             <1..>1307
                     /locus_tag="P5201_14255"
                     /inference="COORDINATES: protein motif:HMM:NF015859.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="CHASE domain-containing protein"
                     /protein_id="HRV99757.1"
                     /translation="VRRLTGSELDLGSPQYESFKNYFRSVLQMYPRSRFLTLVGRRYD
                     GKVFFFVDSEPPGSEDESPPGEVYDAVDPGFLPAFEEGRAVVVGPVVDQWGAWISAWV
                     PLVERGSDRVSAALALDISGEEWQRTLEKAKRLPALTSLFLALLLFAGRWMLQQRTKT
                     GGGAFGRFRYLECLLTLIFGLALTGSVAWMVHERESRSHAQSFSIVAEAHSVKMAGML
                     YRLRNFVLEGTARFIEALPSLEYESFRRYSQHLSSEPEIDALMWSPRVLAEERAEFES
                     TERDEWRRALEIREQNGAGAYVRAEERAEYVPVLFRSPMTGSMFPVGEDLLSHPEVSE
                     ALRTAVNTRRPAAAFVRVQYGKEDGSALWVCYPIFRRGFNGALRGVVSALFLPSSMPD
                     EVMGGSGDAGILSFEALSPEAPPRSIAQWELEGRPEYRFIRPL"
BASE COUNT          227 a          374 c          443 g          263 t
ORIGIN      
        1 cgtgcgccgc cttacgggga gcgaactgga tttagggtcg ccccagtatg aaagtttcaa
       61 gaactatttc cgctcggtct tgcagatgta cccgcggagc cgtttcctca cgctcgtcgg
      121 aaggcgctac gacggcaaag tattcttttt tgtggactcg gaaccgccgg gttcggagga
      181 cgaatcgccc cccggagaag tgtacgacgc agtcgacccg gggtttctgc ccgcattcga
      241 ggagggaagg gccgtcgtcg tcggcccggt cgtggaccag tggggggcat ggatttcggc
      301 gtgggttccc ctggtcgagc gcggttccga cagggtgagc gccgccctgg cgttggatat
      361 ctcgggagag gaatggcaac ggaccctgga gaaagccaag cgccttcccg cgctgacgtc
      421 gctctttctt gcgttgcttc ttttcgccgg acgctggatg ctgcagcagc ggaccaaaac
      481 gggaggagga gcgttcggga gattcaggta tctcgagtgc cttctgacat tgatcttcgg
      541 tcttgccctg acggggagcg tggcctggat ggtgcacgaa cgtgaaagcc gctcccacgc
      601 gcagagtttt tcgatcgtcg cggaagcgca ctccgtcaag atggccggaa tgctctacag
      661 actccgcaat ttcgtcctcg agggaaccgc gcggtttatc gaggcgctcc cttcgctgga
      721 gtacgagtcg ttcaggaggt attcacagca tctgtcgtcg gagccggaga tcgacgcgct
      781 catgtggagt cctcgggtcc tcgcggaaga gcgggcggag tttgaaagca ccgagcggga
      841 cgaatggcgg cgcgcactgg agatacgcga gcagaacggc gccggggcgt acgtccgcgc
      901 ggaggaacgg gcggagtacg ttccggtgct cttccgttcc ccgatgacgg gaagcatgtt
      961 ccctgtgggg gaggatcttc tttcacaccc ggaggtatcg gaagctctca ggacagccgt
     1021 gaataccaga agaccggccg ccgcttttgt acgggtccag tacggaaagg aagacggctc
     1081 cgcgctctgg gtctgctacc caatcttccg tcgaggcttc aacggcgccc tcaggggcgt
     1141 cgtgtcggcg ctctttttgc cgtcttccat gccggatgaa gtcatgggcg ggagcggcga
     1201 cgccgggata ttgagcttcg aggcgctctc tcccgaggcg cccccgcgct cgattgccca
     1261 gtgggagctc gaagggagac cggaataccg cttcatacgt cccctgt
//
LOCUS       DAOTIL010000889         1307 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminobacteriaceae bacterium isolate AMR_MDS_3026
            NODE_123011_length_1307_cov_3.803514, whole genome shotgun
            sequence.
ACCESSION   DAOTIL010000889 DAOTIL010000000
VERSION     DAOTIL010000889.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868050
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminobacteriaceae bacterium (wastewater metagenome)
  ORGANISM  Aminobacteriaceae bacterium
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae.
REFERENCE   1  (bases 1 to 1307)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1307)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:19:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,224
            CDSs (total)                      :: 3,198
            Genes (coding)                    :: 3,184
            CDSs (with protein)               :: 3,184
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1307
                     /organism="Aminobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_123011_length_1307_cov_3.803514"
                     /isolate="AMR_MDS_3026"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:3034609"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            479..>1307
                     /locus_tag="P5201_14260"
     CDS             479..>1307
                     /locus_tag="P5201_14260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008522573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HRV99758.1"
                     /translation="MSSIWGKNFSNIARNNRPSPIREMLAVIKQPGMISFAGGMPAPE
                     IFPIDQFYEGAHILKDDGANLLQYGTTEGYPPLKEFLASWTAPRMGRTVGLDEMLITT
                     GSQQALDLFAWSMIDEGDYVVVEDPSYLAALTVFFNHGGRFLGVPMDEEGMQVDLLPG
                     IIEKARSEGKRIKFIYTIVNFQNPGGSTMSVPRRKQLVEISKKYNIPVFEDDPYGYVR
                     YDGEHLPSIFSFHDEEGVVYAGSFSKILSPGTRVGWIAGPKEIIRNLTVFKQGTDLCS
                     "
BASE COUNT          292 a          377 c          312 g          326 t
ORIGIN      
        1 gttggtacgc gccatggaaa agatgaaagc cgctggattc aagacctccc cgagcggctt
       61 caactcccac cctcctacgg agcgcagact gctccgtctg aggaacaacg cggcggcaat
      121 agccccccgg aactgacaca tttcttaaac acccggagga gaggatgatt cctccgggtg
      181 tagttctttt ttgttgtttt accaccggag ctccttcaca ccttttgaca tgtcctcatc
      241 cggtctctcg ttcggcatcg actacaaacg gccgggaacc agaccgaaat acagtgtgtt
      301 gcgtcaggac tgttttgcag cgcgcacacg ccaatcatcc gatctctctt tttttcttcc
      361 ttttgtcctt ttcttcttcg tgctataatt gatttgtatt atctctcttt tcgagttttt
      421 tttggagctt acgctcagaa agactcaaat ttaccatatc atcaaggggg cgatatgtat
      481 gagcagtatc tggggaaaga acttcagcaa catcgcgagg aacaaccgtc cttcgccaat
      541 tcgcgagatg ctcgccgtta tcaagcagcc cggtatgatc tcttttgccg gggggatgcc
      601 tgcgcccgaa atcttcccta tcgatcagtt ttacgaggga gcgcacattc tgaaggatga
      661 cggcgcaaac ctccttcagt acggcaccac cgaagggtac cctccgctca aggaattcct
      721 ggcttcatgg accgcccccc ggatgggacg cactgtcggt ctcgatgaaa tgcttattac
      781 tacaggatct caacaggccc tcgatctttt cgcatggtcc atgatcgacg agggcgacta
      841 cgtcgtcgtc gaagacccct catacctggc tgccctcact gtcttcttta atcacggcgg
      901 caggttcctc ggcgttccga tggacgagga gggaatgcag gtcgatctcc ttccgggaat
      961 catcgaaaaa gcacgctccg aggggaaaag aataaaattc atctacacca tcgtcaattt
     1021 ccaaaatccg ggcggttcga caatgtccgt tccccggcgc aaacagctcg tggagatttc
     1081 aaaaaaatac aatatccctg tcttcgagga tgacccttac gggtacgttc gttatgacgg
     1141 cgagcatctc ccttcaatct tctcattcca cgacgaggag ggcgttgtct atgccggttc
     1201 cttctcgaag atactctcgc ccggcactcg cgtaggctgg atcgccggcc ccaaagagat
     1261 cattcgcaac ctgaccgttt tcaagcaggg gacggacctc tgctcca
//
LOCUS       DAOTIL010000890         1304 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminobacteriaceae bacterium isolate AMR_MDS_3026
            NODE_123442_length_1304_cov_3.792634, whole genome shotgun
            sequence.
ACCESSION   DAOTIL010000890 DAOTIL010000000
VERSION     DAOTIL010000890.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868050
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminobacteriaceae bacterium (wastewater metagenome)
  ORGANISM  Aminobacteriaceae bacterium
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae.
REFERENCE   1  (bases 1 to 1304)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1304)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:19:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,224
            CDSs (total)                      :: 3,198
            Genes (coding)                    :: 3,184
            CDSs (with protein)               :: 3,184
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1304
                     /organism="Aminobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_123442_length_1304_cov_3.792634"
                     /isolate="AMR_MDS_3026"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:3034609"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..800)
                     /locus_tag="P5201_14265"
     CDS             complement(<1..800)
                     /locus_tag="P5201_14265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008520383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HRV99759.1"
                     /translation="MYDLIVIGGGPGGLRAAELASHAGMSVALVEKDRLGGTCLNRGC
                     IPTKALYAHIVGGKGERDGLWSRVEATMDKLRQGSATALRMAKVKTYRGTATITQWAG
                     EKKVSVRKEDGNVEEIASSRLLVAAGARSVRPSFAGNDLPEVLTGDWAIVDPLLWDPE
                     RNQSVRTVAVLGAGVIALEMAMMLQNLGKKVLLLKHSDQILRRLDGDVKKKVGQIMKK
                     RGTDVREYVRLSEAVRDGGGLLLRGTAKDEPFEERCDRLLLASSMEPIL"
     gene            complement(884..>1304)
                     /locus_tag="P5201_14270"
     CDS             complement(884..>1304)
                     /locus_tag="P5201_14270"
                     /EC_number="1.4.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008520379.1"
                     /note="acts in conjunction with GvcH to form
                     H-protein-S-aminomethyldihydrolipoyllysine from glycine;
                     forms a heterodimer with subunit 1 to form the P protein;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="aminomethyl-transferring glycine dehydrogenase
                     subunit GcvPB"
                     /protein_id="HRV99760.1"
                     /translation="SETAVLNANYLAKKLEHIYPLAHKGYCMHEFVLNGGALKHETGV
                     SSLDVAKALIDAGYHPPTVYFPLIVPEALMVEPTETESQETLDRFAAAMEEIVAKAKA
                     EGAEPFHDMPLTTPVSRPDETTAARNPILRWQFGDAE"
BASE COUNT          175 a          475 c          406 g          248 t
ORIGIN      
        1 aggatcggct ccatgctgga ggccagcagc agccggtcgc accgctcctc gaacggctcg
       61 tccttcgcgg ttccccggag gagcagcccg ccgccgtccc gcacggcttc ggagaggcgc
      121 acgtactcgc ggacgtccgt gccgcgtttc ttcatgatct ggcctacctt cttcttgacg
      181 tccccgtcga ggcggcgcag aatctggtcg gagtgcttca gcaggaggac cttcttcccc
      241 aggttctgga gcatcatcgc catttcgagg gcgatgaccc ccgcgccgag caccgcgacc
      301 gtccgcacgc tctgattgcg ctccgggtcc cagaggagcg ggtcgacgat ggcccagtcg
      361 cccgtcagca cctccggcag atcgttcccc gcgaacgacg gtctgacgga gcgcgcgccc
      421 gcggcgacga gcagccggga cgaagcaatc tcttcgacgt tcccgtcctc cttgcggacg
      481 gagaccttct tctctcccgc ccactgcgtt atcgtggccg ttcccctgta cgtcttcacc
      541 ttcgccatgc gcagcgcggt ggccgagccc tggcgtagct tgtccatcgt cgcctccacg
      601 cggctccaga gtccgtcccg ttcacccttg ccgccgacga tatgcgcgta gagcgccttg
      661 gtggggatgc accccctgtt gaggcaggtt ccgccgaggc ggtccttctc gaccagcgcc
      721 acgctcatcc ccgcgtgcga cgccagttcc gcggcgcgaa gccctcccgg gccgccgccg
      781 atgacaatca ggtcgtacat gctctctctc cttccctgtg cggtactgaa aagggggcgt
      841 cgcacacgcg gcgcgccccc tcgatgatac tgtcgctgcg cggttactcc gcgtccccga
      901 actgccagcg gagaatcgga ttccgcgccg ccgtcgtctc gtccgggcgg gagacgggcg
      961 tcgtcagggg catgtcgtgg aacggttccg ccccctcggc cttcgccttc gccacgatct
     1021 cctccatggc cgccgcgaac cggtcgagcg tctcctgcga ctccgtctcc gtgggctcga
     1081 ccatcagcgc ctccgggacg atgagcggga agtagaccgt cggcggatgg taccccgcgt
     1141 cgatcagcgc cttggccacg tcgaggctgc ttactccggt ctcgtgcttc agcgcgcctc
     1201 cgttgaggac gaactcgtgc atgcagtacc ccttgtgcgc gagcgggtag atgtgctcga
     1261 gctttttggc gaggtagttc gcgttgagca ccgccgtctc ggag
//
LOCUS       DAOTIL010000891         1303 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminobacteriaceae bacterium isolate AMR_MDS_3026
            NODE_123616_length_1303_cov_2.982372, whole genome shotgun
            sequence.
ACCESSION   DAOTIL010000891 DAOTIL010000000
VERSION     DAOTIL010000891.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868050
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminobacteriaceae bacterium (wastewater metagenome)
  ORGANISM  Aminobacteriaceae bacterium
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae.
REFERENCE   1  (bases 1 to 1303)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1303)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:19:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,224
            CDSs (total)                      :: 3,198
            Genes (coding)                    :: 3,184
            CDSs (with protein)               :: 3,184
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1303
                     /organism="Aminobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_123616_length_1303_cov_2.982372"
                     /isolate="AMR_MDS_3026"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:3034609"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..303
                     /locus_tag="P5201_14275"
     CDS             <1..303
                     /locus_tag="P5201_14275"
                     /inference="COORDINATES: protein motif:HMM:NF016432.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma factor-like helix-turn-helix DNA-binding
                     protein"
                     /protein_id="HRV99761.1"
                     /translation="MINFLQRSEKKAPLPVDDEFLMPEDPFLPETFETMLALSEELRR
                     LPAKEGEVLQAILMDGREAREVAQERGIDISHVYRLKRNALAKLRKWLGAEATNEV"
     gene            385..1143
                     /locus_tag="P5201_14280"
     CDS             385..1143
                     /locus_tag="P5201_14280"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRV99762.1"
                     /translation="MEKKIARIYRWLERCAKACSAGSWNSALLDMECAKAELEDVRTE
                     IWSKTEGVFEVRPTRRGALALKASFLSVVLLFSLAAPLAIQSSRPASSVYQASGNVHS
                     GEHVLEWVTGDEQAVLAALRKSLSDANRAWSAAAFRGETDAVAGESASAPAMDEDPVA
                     EEKTYVARAPSASEGSGASRESGAETVSRRVRPKAQGELAAGSAGLKNEVVAGEDAIR
                     PSSVANELDSIISLVQIGQKALRERESPIQFQRP"
BASE COUNT          290 a          310 c          408 g          295 t
ORIGIN      
        1 atgatcaact tcctgcagcg ctcagaaaaa aaggctcctc ttcctgtgga cgatgagttc
       61 ctgatgccgg aggacccttt ccttcccgaa acgttcgaga ccatgcttgc tctttcggaa
      121 gagttacgac ggctcccggc aaaggagggg gaggtgctgc aggcaattct tatggatgga
      181 cgggaggcaa gagaggtagc gcaggagcgc ggaatcgata tcagtcacgt ctatcgtctg
      241 aagaggaatg cgctggcgaa acttcgcaaa tggcttggcg ccgaggccac aaacgaggtc
      301 taaaacggga taagagaata cagtgaccgc ccacgcgcgt ttttttgctt gaaagcgtgg
      361 agaacattcc cgggaggact ttccatggag aagaagatcg ctcgaattta tagatggctt
      421 gaacgatgcg cgaaggcatg ttccgcaggt tcttggaaca gcgcgctgct tgatatggag
      481 tgcgcgaaag ccgagctgga agatgtccgg acggaaatct ggtcgaagac cgagggggtt
      541 ttcgaggtgc ggccgaccag gagaggcgct ctcgccctga aggcgtcttt tttgagcgtc
      601 gtccttcttt tttcccttgc cgcgccgctt gcgatacaga gttcgagacc ggcgtcttct
      661 gtgtatcagg cgagcggaaa tgttcactcg ggagagcatg tccttgaatg ggtgactggc
      721 gacgagcagg ctgttcttgc cgctcttcgt aaaagcctga gcgatgcgaa tcgagcgtgg
      781 agtgcggctg cgtttcgagg tgaaaccgac gcggtcgctg gtgaatcggc gagcgccccc
      841 gcaatggacg aagaccctgt tgcggaggag aaaacctatg tcgcccgcgc tccgtctgcg
      901 tcggaaggaa gcggagcttc ccgcgagtcc ggagcagaaa ctgtttcccg ccgggtgcgt
      961 ccgaaagctc agggagaact tgcggcaggc tcagctggac tgaagaacga agtcgtcgcc
     1021 ggtgaagacg ctatccgccc ttcctcagtg gcaaatgaac tcgattcaat aatctccctt
     1081 gtgcagatag gacaaaaagc cttgcgcgag agggagtctc cgatacagtt tcaacgtccg
     1141 tgaagcaaac ggccggttat tttttggact tctgattttg ctttgttgat ggcgtgcgtt
     1201 ttacagagtg tatatttttt cgtcccgttc aggacgaatt catttttgtt ttttggaagg
     1261 agagagcgtg cgtgaaagga aaaattggaa ggtcctggcc ggc
//
LOCUS       DAOTIL010000892         1303 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminobacteriaceae bacterium isolate AMR_MDS_3026
            NODE_123666_length_1303_cov_2.153846, whole genome shotgun
            sequence.
ACCESSION   DAOTIL010000892 DAOTIL010000000
VERSION     DAOTIL010000892.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868050
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminobacteriaceae bacterium (wastewater metagenome)
  ORGANISM  Aminobacteriaceae bacterium
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae.
REFERENCE   1  (bases 1 to 1303)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1303)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:19:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,224
            CDSs (total)                      :: 3,198
            Genes (coding)                    :: 3,184
            CDSs (with protein)               :: 3,184
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1303
                     /organism="Aminobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_123666_length_1303_cov_2.153846"
                     /isolate="AMR_MDS_3026"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:3034609"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>1303
                     /locus_tag="P5201_14285"
     CDS             <1..>1303
                     /locus_tag="P5201_14285"
                     /inference="COORDINATES: protein motif:HMM:NF012771.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRV99763.1"
                     /translation="AYFYHLPSELAGIESFPTVARNGCVDLELRLVYRASLPLSFGDK
                     LTGRNGNKGVVTRILRDNERPVARIAGRDVPVDLMISPCSILGRKNLGQILEMLHALL
                     LKGEEWGTPMGWHAPRDRSMTSGELLEKALPELQASFGADEEGLFPLCFPDGRYARAL
                     VAPKYFLRLHHHGVKKLQARGASGPMNQATGLPDRGGARTAQRMGEMEHWSILSYPDG
                     RGKDLLLSLREKRGSGEGNERMRSLLRSILTGLGLYLEKKEGTVGLRPLTADSADAMR
                     KKGVVVEEMDVFRADQEIRKLSVDKAEEMKNTVWRISSVSADSPAPGAVVEKILGERY
                     SLFGKDGALWVPLDLLFLHRELLGQSGRELPKTGLLRLLYEVKRWAKSGGEKKHVKKT
                     LNQYRKSLVYLLAGKEGLVRQSMMGRRFSASGRGVIVPEPDL"
BASE COUNT          271 a          300 c          445 g          287 t
ORIGIN      
        1 cgcgtatttc tatcatctgc cgtccgaact tgccggcata gagtctttcc ccacagttgc
       61 caggaacggg tgtgtcgatc tggagctgcg tcttgtctac agggcttctt taccgttgag
      121 tttcggggat aagttgactg gaagaaacgg caataagggc gtcgtgacgc gtattctgcg
      181 ggacaacgag cgtcctgtgg ccaggatagc cgggcgtgat gtcccggtgg atttgatgat
      241 ctcgccatgc tctattctgg ggcggaaaaa cttggggcag attttggaga tgctgcacgc
      301 tcttctgctg aaaggggaag agtgggggac gccgatgggg tggcatgctc caagagatcg
      361 ctcgatgacg tccggggagc tgctggagaa ggcgcttcct gagcttcagg cgtcattcgg
      421 agccgacgaa gaagggctct ttccgctgtg ttttccagac ggaaggtatg cgcgggcttt
      481 agtggcgccc aagtattttt tgcggctgca tcatcacggg gttaaaaaac tccaagccag
      541 gggagcttcg gggcctatga atcaagcgac gggcttgccc gaccggggag gggcgagaac
      601 ggcgcagcgg atgggggaga tggagcactg gtccattttg agctatccgg acggcagggg
      661 gaaggatctt cttctctcgc ttcgtgaaaa acggggttcc ggtgaaggaa acgaacggat
      721 gcgatctctc cttcgttcaa tactgacggg gctcggactc tacctggaga agaaggaggg
      781 gactgtcggt ctgcgcccgc tgaccgcgga ctccgcggat gcgatgcgaa aaaaaggcgt
      841 cgtcgtcgag gagatggatg tcttccgggc ggatcaggag attcgcaagc tgtcagtgga
      901 caaagcggaa gaaatgaaga acacggtgtg gaggatctct tccgtatccg cggattctcc
      961 ggctccgggg gccgtagtgg aaaaaattct tggagaacga tatagcctct tcggtaagga
     1021 cggggcgctt tgggtacctc tggatctgct tttcttgcac cgcgagctgc ttggccagtc
     1081 ggggcgggaa cttcctaaaa cgggtctttt gcggcttttg tacgaagtga agcgctgggc
     1141 gaaatccgga ggagagaaga agcatgtaaa gaagactctg aatcagtacc ggaaatccct
     1201 tgtttatctc cttgcaggaa aggaggggct ggtgcgacag tcgatgatgg ggcgccgctt
     1261 ttcggcgagc ggacggggtg tgatcgtacc cgagcccgat ctg
//
LOCUS       DAOTIL010000893         1300 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminobacteriaceae bacterium isolate AMR_MDS_3026
            NODE_124021_length_1300_cov_3.558233, whole genome shotgun
            sequence.
ACCESSION   DAOTIL010000893 DAOTIL010000000
VERSION     DAOTIL010000893.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868050
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminobacteriaceae bacterium (wastewater metagenome)
  ORGANISM  Aminobacteriaceae bacterium
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae.
REFERENCE   1  (bases 1 to 1300)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1300)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:19:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,224
            CDSs (total)                      :: 3,198
            Genes (coding)                    :: 3,184
            CDSs (with protein)               :: 3,184
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1300
                     /organism="Aminobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_124021_length_1300_cov_3.558233"
                     /isolate="AMR_MDS_3026"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:3034609"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..795
                     /locus_tag="P5201_14290"
     CDS             <1..795
                     /locus_tag="P5201_14290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009202286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional enoyl-CoA hydratase/phosphate
                     acetyltransferase"
                     /protein_id="HRV99764.1"
                     /translation="QREGIVRTILIGDEGKIRTLAGRDGYDLSDIAIVDEKNDDTAVE
                     RSVKMVSSGEADLLMKGLVKTSSLLKAVLDKEWGLRTGSLLSHLFLFEVPTLGGRVIG
                     ISDGGMNTYPDLNAKVKIVENAVACYHRIGVPQPKIAALAAVEVVNPDMPATLEAAAL
                     AKMNERGQIKGCLVDGPFALDNAVSEESARIKGISSPVAGNADMLLVPDIESGNFVGK
                     VLLYMTGGKGAGVILGARKPIVLTSRFDSMETKLLSIALGAVVARS"
     gene            831..>1300
                     /locus_tag="P5201_14295"
     CDS             831..>1300
                     /locus_tag="P5201_14295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008520894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acyltransferase"
                     /protein_id="HRV99765.1"
                     /translation="MEQIRSLSQLLDYAKKIGAEKGPKKLSVAMAEEAGLLTAIEEAR
                     KAGFAEAILVGNGDKMKAAAEQAGVDLANYEVIDERDEPSMGISAVSQVSSKKADIYM
                     KGQIHTNNFLRAMLSKEVGLRKGKNTISHCYFHSVEGYDRIFFIADAAFNMYPDL"
BASE COUNT          275 a          400 c          410 g          215 t
ORIGIN      
        1 cagcgtgaag ggatcgtcag gacgatcctc atcggcgacg aggggaagat acgcaccctc
       61 gcggggcgag acggctacga tctctcggat atcgcaatcg tcgacgagaa aaacgacgac
      121 acagcggtgg agcggagcgt caagatggtc tcctccgggg aggccgacct gctgatgaag
      181 gggctcgtga agacgtcgtc gctgctgaag gctgtgctcg acaaggagtg ggggctacgg
      241 accggttcgc tgctctccca cctcttcctc ttcgaagttc ccacgctcgg aggccgggtg
      301 atcggcatct ccgacggcgg gatgaacacc taccccgacc tgaacgcgaa ggtgaagatc
      361 gtcgagaacg cggttgcctg ctaccacagg atcggcgtcc cgcagcctaa gatcgccgcg
      421 ctcgccgccg tcgaggtggt gaaccccgac atgcccgcaa cgctcgaagc cgccgcgctc
      481 gccaaaatga acgagcgcgg gcagatcaag gggtgcctgg tcgacggtcc ctttgcgctg
      541 gacaacgcgg tcagcgagga gtccgcgcgc atcaagggaa tctcctcgcc cgtggccggc
      601 aacgccgaca tgctgctggt tcccgacatc gagtcgggca acttcgtcgg aaaggtgctg
      661 ctctacatga cgggcggcaa gggggcgggc gtcatcctcg gcgcgcgcaa gcccatcgtc
      721 ctcaccagcc gcttcgacag catggagaca aaattgcttt caatcgccct gggcgcggtc
      781 gtcgcccgga gctaaaaaag aatatttcat tcagaggagg agtttttgtt atggaacaga
      841 ttcgctcgct ttcccagctt ttggactacg caaagaagat cggcgccgag aaggggccga
      901 agaagctctc ggtcgccatg gccgaggagg ccggtctgct gaccgccatc gaggaggcgc
      961 gcaaggccgg attcgccgag gcgatcctcg tgggcaacgg cgacaagatg aaggctgcag
     1021 cggagcaggc aggagtcgac ctcgccaact acgaggtcat cgacgagcgc gacgagccct
     1081 cgatggggat ctccgccgtc tcccaggtct cttccaagaa ggccgacatc tacatgaagg
     1141 gccagatcca cacgaacaac ttcctccgcg cgatgctcag caaggaagtc gggctccgga
     1201 aggggaagaa caccatctcc cactgctact tccacagcgt tgaagggtac gaccgcatct
     1261 tcttcatcgc cgacgccgcc ttcaacatgt atccggatct
//
LOCUS       DAOTIL010000894         1299 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminobacteriaceae bacterium isolate AMR_MDS_3026
            NODE_124237_length_1299_cov_2.418810, whole genome shotgun
            sequence.
ACCESSION   DAOTIL010000894 DAOTIL010000000
VERSION     DAOTIL010000894.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868050
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminobacteriaceae bacterium (wastewater metagenome)
  ORGANISM  Aminobacteriaceae bacterium
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae.
REFERENCE   1  (bases 1 to 1299)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1299)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:19:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,224
            CDSs (total)                      :: 3,198
            Genes (coding)                    :: 3,184
            CDSs (with protein)               :: 3,184
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1299
                     /organism="Aminobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_124237_length_1299_cov_2.418810"
                     /isolate="AMR_MDS_3026"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:3034609"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            377..>1299
                     /locus_tag="P5201_14300"
     CDS             377..>1299
                     /locus_tag="P5201_14300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014806717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA translocase FtsK"
                     /protein_id="HRV99766.1"
                     /translation="MINALRSLSLPRRGIIDTEDVHQKETPVEKTETENESGLPSRFV
                     LVRPPSHGIPADVLQRIEETQLSEERLPQEDTEGFEEEKNGALFGFFKRKRKTLPEDE
                     PLLTENSIVALTPHEARSEALPEQPDAALPEVFETHSDREEMSEVDDTDTDTDIEMEE
                     DVFLLRRPEENISGQGLQTELPEDDAVVAKIEQDSEDVLTDPHALPDIEFHVVEEEGR
                     VRSGTFPPPPEIFGPRQEMETSDDNETVREQASSIITTLADFGVSAEMADIVIGPTVI
                     QFQLQLAPGIKVSKVAGLSNDLAVALTVPSLR"
BASE COUNT          304 a          323 c          341 g          331 t
ORIGIN      
        1 cgtttggggg gatgcgatcc gtttttctct actgcgaaac tggggcggcg ctatcctcat
       61 tcctgttttt ttcggggggt atctatgcat tgcctatttt ttaaagacag gcgcaaaagg
      121 gtttctctcg caagcactgg gtacgtttct tcttttttta tgtactgccc ttctgttcgg
      181 gctctttcag acaacccgta tttttcaagg tttttcgtta tttactcccg ggtatctagg
      241 tcaaggtctg gcggtctttc ttaccaggga gataggggcc cttggaacaa cgctccttgc
      301 cgtggcgttg cttattcttt ctgcaaccct ctatggcttt attcatccgg cttctgtaat
      361 atccaggatt tcaaagatga tcaatgctct tcgttctctt tcgttaccga gacgcggcat
      421 cattgatact gaagatgtac accaaaaaga aacccccgtt gagaagacgg aaacggaaaa
      481 cgaatccggg cttccctccc gttttgttct ggtgcgtccc ccctctcacg gtattcctgc
      541 ggatgtgctg cagcgaatag aagaaacgca gctttcagaa gagcggcttc ctcaggagga
      601 caccgaagga ttcgaagaag aaaagaacgg ggcgcttttt ggttttttca agcggaaaag
      661 aaaaacgctc cctgaggatg agcctcttct tactgaaaac tcgatagttg cgctcactcc
      721 acacgaagcg aggagcgaag ccctccccga gcagcccgat gctgcgcttc ctgaagtttt
      781 cgaaactcat tcagaccggg aagaaatgag cgaagtggac gataccgaca ccgacacaga
      841 tattgagatg gaggaggatg tcttcctcct gagacgaccg gaggagaaca tatcaggaca
      901 aggactgcaa acggagcttc cggaggacga tgcagtcgtc gcgaaaattg agcaagacag
      961 cgaagatgtt ctcacagatc cgcacgctct gcccgatatt gagtttcatg tggtggaaga
     1021 agaaggtcgt gtccggagcg gcacattccc tcctccgccg gaaattttcg gtcccaggca
     1081 ggagatggag acatcggatg ataacgaaac cgttcgagaa caagcttcgt cgattatcac
     1141 cacgctcgcg gattttggcg taagtgcgga gatggcggat atcgtcatcg gtccgacggt
     1201 tatccagttc caactacaac ttgctccggg aataaaggtg agcaaggttg ccggtttgag
     1261 caacgatctt gctgttgcgc tcaccgtgcc ttcgctccg
//
LOCUS       DAOTIL010000895         1298 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminobacteriaceae bacterium isolate AMR_MDS_3026
            NODE_124301_length_1298_cov_4.304907, whole genome shotgun
            sequence.
ACCESSION   DAOTIL010000895 DAOTIL010000000
VERSION     DAOTIL010000895.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868050
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminobacteriaceae bacterium (wastewater metagenome)
  ORGANISM  Aminobacteriaceae bacterium
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae.
REFERENCE   1  (bases 1 to 1298)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1298)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:19:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,224
            CDSs (total)                      :: 3,198
            Genes (coding)                    :: 3,184
            CDSs (with protein)               :: 3,184
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1298
                     /organism="Aminobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_124301_length_1298_cov_4.304907"
                     /isolate="AMR_MDS_3026"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:3034609"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1298)
                     /locus_tag="P5201_14305"
     CDS             complement(<1..>1298)
                     /locus_tag="P5201_14305"
                     /inference="COORDINATES: protein motif:HMM:NF012245.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HRV99767.1"
                     /translation="KGKIIFTEPYVDGITKQVVISTATAIYDDAGKLLGVVGGDMDIS
                     GLSDYVVGLQIFGKGSGLMLLKSGIFVAYNEKDDVLKANMMTDEKFAEPIRNIARKMV
                     SGETGYESYVYQGEERQMFFAPTQRAFYLGILFPAAEINAMVNALTFILLGLAVVAVL
                     VTGGTIFFIARGLTKSIRSMESVTEKLGEGDLTVSYEASGKDEIAHISGILNGMVFSL
                     REVMGSIRNESQETAARAETLASLSEETLASMEEVSSSIEKVQDMMEHSSSALQETNA
                     SIEEIAAGAQTAARATNEGADGATNASDAANASLQEVGRAINNIRNAGSESAKSIDRI
                     KELGKSVEAISGFVTTITSIADQTNLLALNAAIEAARAGEAGRGFAVVAEEVRKLAEE
                     SARAAHEVSKLINELQKHSDDSIAATEKTGTILDDTMKGA"
BASE COUNT          204 a          432 c          365 g          297 t
ORIGIN      
        1 ccgcgccctt catcgtatcg tccagtatag ttcccgtctt ttccgtggct gcgatggagt
       61 cgtccgaatg cttctgaagc tcgttgatga gcttgcttac ctcgtgcgcc gccctggccg
      121 actcctcggc gagcttgcgg acctcttcgg ccaccaccgc gaagcctctt ccggcctcgc
      181 cggctctggc cgcttctatc gccgcgttca gcgccagcag gttcgtctgg tccgctatcg
      241 acgtgatggt cgtcacgaat ccggagatag cctccaccga cttgccgagc tccttgatcc
      301 ggtcgatgct cttcgcggac tcgctccccg cgttccgtat gttgttgatt gcgcgtccta
      361 cctcctgcag cgaagcgttc gcggcgtcgg aggcgttcgt cgcgccgtcc gctccttcgt
      421 tggtcgccct tgccgcggtc tgcgcgcccg ccgcgatctc ctctatcgac gcattcgtct
      481 cctgcagcgc ggaggagctg tgctccatca tgtcctggac tttttcaatg gagctggaga
      541 cctcttccat ggacgcaagc gtctcctcgg agagggaagc gagcgtctcg gcgcgcgcgg
      601 ccgtctcctg cgactcgttt cggatcgatc ccatgacttc gcgcagcgag aagaccatgc
      661 cgttcagaat gccggaaata tgcgcgatct cgtctttgcc cgaggcctcg tacgagaccg
      721 tcaggtcgcc ttctcccagc ttctccgtca cactctccat cgagcgtatc gacttcgtca
      781 ggccgcgcgc gatgaagaag atcgtccctc ccgtcaccag gacggcgacg accgcgagac
      841 cgagcagaat aaaggtgagc gcgttcacca tcgcgttgat ctccgcggcg gggaagagga
      901 tgccgaggta gaaagcgcgc tgcgtagggg cgaagaacat ctgccgctcc tccccctggt
      961 agacgtagct ctcatatccc gtctcgccgg agaccatctt ccgcgcgata ttccgtatcg
     1021 gctcggcgaa cttctcgtcg gtcatcatgt tcgccttcaa aacgtcgtct ttctcattgt
     1081 aggcaacgaa gatgccgctt ttcaggagca tcaggccgct tcccttgccg aaaatctgga
     1141 gcccgacgac gtagtcgctc agaccgctga tgtccatgtc gcccccgacg actcccagga
     1201 gttttccggc gtcatcgtag atagccgtgg cggtggagat gacgacctgc ttcgtgatcc
     1261 cgtcgacgta aggctcggtg aagatgatct ttcccttt
//
LOCUS       DAOTIL010000896         1297 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminobacteriaceae bacterium isolate AMR_MDS_3026
            NODE_124458_length_1297_cov_3.481481, whole genome shotgun
            sequence.
ACCESSION   DAOTIL010000896 DAOTIL010000000
VERSION     DAOTIL010000896.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868050
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminobacteriaceae bacterium (wastewater metagenome)
  ORGANISM  Aminobacteriaceae bacterium
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae.
REFERENCE   1  (bases 1 to 1297)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1297)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:19:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,224
            CDSs (total)                      :: 3,198
            Genes (coding)                    :: 3,184
            CDSs (with protein)               :: 3,184
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1297
                     /organism="Aminobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_124458_length_1297_cov_3.481481"
                     /isolate="AMR_MDS_3026"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:3034609"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(217..>1297)
                     /gene="argH"
                     /locus_tag="P5201_14310"
     CDS             complement(217..>1297)
                     /gene="argH"
                     /locus_tag="P5201_14310"
                     /EC_number="4.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013048892.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004056 - argininosuccinate lyase activity
                     [Evidence IEA];
                     GO_process: GO:0042450 - arginine biosynthetic process via
                     ornithine [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="argininosuccinate lyase"
                     /protein_id="HRV99768.1"
                     /translation="ATTMRLFLRDRLLNIAEGMERLLAELLRNAAKHKDTIVAGYTHL
                     QQAQPVSMGHYWMAHFAAFSRDVSRLFFALDSLNECPLGAGALAGSTLPLDRSYTSSL
                     LGFTGPTRNSLDTVAQRDYMVDYHSFAAVFAVHCSRLAEDFVIYSSREFGWLLLPDAF
                     CTGSSMMPQKKNPDVMELIRGKTGQIIGHLLDLLITLKALPMTYDRDLQEDKRGLRAS
                     LNTVDQILAVLPPLLAAVDVDGKHAVEGLEDGFALATDVAEYLVLKGVPFRDAHAMTG
                     RLVKECIARSKGLFDLSREEWDELLPEAGDDLFPMLALEKAVERRKTYGGTSPTEVVR
                     QIEHGEALLSAQRARRAEFVRTVEL"
BASE COUNT          265 a          389 c          373 g          270 t
ORIGIN      
        1 gacacccgga ttaaaagtcc ggtgctctgc catctgagct aacggctcga ctcttcctga
       61 gtgcgcaagt gattatttta cacaaaaaaa cgcgaacgtc aagcggtcgg gtgttttttt
      121 acgcccttta ttttaaagtt tttactcagg gccttctctc ttcagtacca ttcgcccccg
      181 ccggaagctc tgtccggtcg cgtatgctcg ctcttctcaa agctccacgg tccggacgaa
      241 ctccgctctt cttgcgcgct gagcggaaag aagcgcctcg ccatgctcga tttgccgtac
      301 aacttccgtc ggggatgtgc ctccgtaggt tttccgtctc tcgaccgcct tttccaaagc
      361 gagcattggg aaaagatcgt ctcccgcctc ggggagcaat tcgtcccact cctctctgga
      421 gagatcaaaa agccccttac tccgggcgat gcattctttc accagacgcc ctgtcatggc
      481 atgggcatcc cggaagggaa cgcccttcaa gacgagatac tcggccacgt cggttgccaa
      541 tgcaaagccg tcctccagcc cctcgacagc gtgtttccca tcgacgtcca ccgcggccag
      601 gagcgggggg agcactgcga ggatctggtc taccgtattc agcgaagcgc gcagtcctcg
      661 cttgtcttcc tgcaaatccc ggtcgtaggt catcggaagc gccttcagag taatcaggag
      721 gtcgagcaga tgcccgatga tctgccccgt cttcccgcgg atcagctcca tcacatcagg
      781 gttcttcttt tgaggcatca tgctggatcc tgtgcagaag gcgtctggaa gcagaagcca
      841 cccgaactcc ctcgaggagt agatgacgaa atcctcggca agccggctgc aatggacggc
      901 aaagactgcg gcaaaggagt gatagtcgac catgtagtcc ctttgggcaa ccgtatcaag
      961 gctgttgcgc gtcggcccgg tgaacccgag cagagaggag gtgtaggagc ggtcgagagg
     1021 aagggtggac ccggcgagcg ctccggcccc cagcgggcac tcgttcagcg aatcgagcgc
     1081 gaagaagagg cgggagacat ctctggagaa cgcggcgaag tgcgccatcc agtaatgccc
     1141 catcgagacg ggttgggctt gctgcaagtg ggtgtacccc gcgacgatag tgtccttgtg
     1201 tttcgcggca ttgcgcagca gctccgcgag caggcgctcc ataccctcgg cgatattcag
     1261 gagcctgtcc cgaagaaaga gacgcatcgt ggtcgcc
//
LOCUS       DAOTIL010000897         1297 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminobacteriaceae bacterium isolate AMR_MDS_3026
            NODE_124461_length_1297_cov_3.341385, whole genome shotgun
            sequence.
ACCESSION   DAOTIL010000897 DAOTIL010000000
VERSION     DAOTIL010000897.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868050
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminobacteriaceae bacterium (wastewater metagenome)
  ORGANISM  Aminobacteriaceae bacterium
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae.
REFERENCE   1  (bases 1 to 1297)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1297)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:19:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,224
            CDSs (total)                      :: 3,198
            Genes (coding)                    :: 3,184
            CDSs (with protein)               :: 3,184
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1297
                     /organism="Aminobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_124461_length_1297_cov_3.341385"
                     /isolate="AMR_MDS_3026"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:3034609"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..320)
                     /locus_tag="P5201_14315"
     CDS             complement(<1..320)
                     /locus_tag="P5201_14315"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRV99769.1"
                     /translation="MTRRFLAIRPNDGNMPLNVFPSVDRMMRDMMRVFGDFSSDVGYP
                     ADAEKMLAIPRGDFYRKDGKIFAEFEIPGIDPSKVELNVYEDRLELRAEKSDEKTLEQ
                     NNVLR"
     gene            600..1181
                     /locus_tag="P5201_14320"
     CDS             600..1181
                     /locus_tag="P5201_14320"
                     /EC_number="1.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013047606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016646 - oxidoreductase activity, acting
                     on the CH-NH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavin reductase family protein"
                     /protein_id="HRV99770.1"
                     /translation="MKVAVNKPLPVYPAPAFLVGVYDEEGKPGGMIAAWGGVCCSEPP
                     CISVAVQKARYSFGGIVARKAFTVNVPSEAQAAEADYFGIFSGRDGDKFAATGLTPRR
                     GEIADAPLVEEFPISFECEVIHSAELGSHVLFVGKVVKTWAFEEHLDERGQPDPLKVR
                     PLVFAPQFGMYYGLRDEPVARAFSVGRTIREGK"
BASE COUNT          287 a          382 c          335 g          293 t
ORIGIN      
        1 cggagaacat tgttctgctc caaggttttc tcgtcggatt tctccgcccg gagttcgagc
       61 ctgtcctcgt agacgttcaa ctccaccttg gaaggatcga tgcccggaat ctcgaactcg
      121 gcgaaaatct ttccgtcctt cctgtagaag tcgcccctgg gaatagcgag catcttctcc
      181 gcatccgcag gataccccac gtcggatgaa aaatcgccga aaacccgcat catgtcacgc
      241 atcattctgt cgaccgaagg aaaaacattc aagggcatat tgccgtcgtt cggcctgatg
      301 gccagaaatc tccgtgtcat ttgaaacacc tccgtttgat ctttattgat tttgatcttt
      361 actgaccttt gaccttatta tatacgtcag cattggtcag acaatcctga aataccgtat
      421 tgaggcgtaa atacatataa ataccatagg aaatgacctt ctatcttttg ttttcacatc
      481 gaaaaaacga attcctcaca ttttaagaga agaccctccg ggagtttctt cgcgatgtgc
      541 gttacaatac actcgatgcg cgcagcatcg cgcgcactct ttgcaaagga gctgatctca
      601 tgaaggtagc agtcaacaaa cctcttcccg tgtatccggc ccccgcgttc ctcgtcggcg
      661 tgtacgacga agaaggaaaa cccggaggca tgatcgccgc atggggcggc gtctgctgtt
      721 cggagccccc ctgcatctcc gtcgccgtac agaaggctcg gtacagcttc ggcggcatcg
      781 tagcgcggaa ggcgtttacc gtaaacgttc catccgaggc gcaggccgcc gaggccgact
      841 acttcggcat tttttcgggg cgcgacggcg acaagttcgc tgccaccggg ctcacccctc
      901 gccgcggaga aatcgcggac gcgccgctgg tggaggagtt ccctatctct ttcgagtgcg
      961 aagtgattca ctcggccgaa cttggttccc acgtgctctt cgtcggcaag gtcgtcaaaa
     1021 catgggcgtt cgaggagcat ctcgacgaga gaggacagcc cgacccgctg aaagtgcgcc
     1081 ccctcgtctt cgcgccgcag ttcggaatgt actacgggct gcgggacgaa cctgtggcga
     1141 gagccttctc cgttggcaga accatccggg agggaaaata aaaagaaggg gccgagaacg
     1201 gccccttcgt tcatattacg ttaccgcagc ccctgtcctc taagaaaccg cctcgcgctc
     1261 ggcggagaag gcttcgaact cgcgcccctt cacgatc
//
LOCUS       DAOTIL010000898         1293 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminobacteriaceae bacterium isolate AMR_MDS_3026
            NODE_125042_length_1293_cov_3.106624, whole genome shotgun
            sequence.
ACCESSION   DAOTIL010000898 DAOTIL010000000
VERSION     DAOTIL010000898.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868050
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminobacteriaceae bacterium (wastewater metagenome)
  ORGANISM  Aminobacteriaceae bacterium
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae.
REFERENCE   1  (bases 1 to 1293)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1293)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:19:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,224
            CDSs (total)                      :: 3,198
            Genes (coding)                    :: 3,184
            CDSs (with protein)               :: 3,184
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1293
                     /organism="Aminobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_125042_length_1293_cov_3.106624"
                     /isolate="AMR_MDS_3026"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:3034609"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1056)
                     /locus_tag="P5201_14325"
     CDS             complement(<1..1056)
                     /locus_tag="P5201_14325"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRV99771.1"
                     /translation="MKTTTRTPGSLPTGQTAEKGFRSSWNGSRYREFLLLIAPLLLFS
                     AYPLLRSFEHAGNSGVFGGVRMTGALIALSAGCAFIARFAGPGRRFYLFAGLAFFVAG
                     AEDLAIGFLELAGPFFAQAPREAVLRSSIAFRTLTPLLLMAPLVISAGKGVPRRPGRE
                     LLPALLSVPLIIALTLYAALNGSPLPFHTLLSRPLDFLSAALFTAALIVYAREFRRSG
                     ESITWWLGLSIGVMLAGQSLLLFSREIYDPFFAAARVCSILGYAASLLGFSLHQLSVE
                     DDQNWTVFRMSAEVDRINAAYLEDVVLEKIREVADSHMATILAMSKLAESRDDETGRH
                     IERTQELCRVLARKMREL"
     gene            complement(1093..>1293)
                     /locus_tag="P5201_14330"
     CDS             complement(1093..>1293)
                     /locus_tag="P5201_14330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012870115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator"
                     /protein_id="HRV99772.1"
                     /translation="LVDVYDALRSDRPYKEAFPHETACRIIREGNGTHFDPSVVDAFF
                     AVEQEFEQICDHMTNEGAEGIL"
BASE COUNT          248 a          412 c          430 g          203 t
ORIGIN      
        1 gagttcccgc atcttgcggg ccagaacgcg gcagagctcc tgcgtgcgct cgatgtgtct
       61 tcccgtttcg tcgtccctcg actccgccag cttcgacatg gcgaggatcg tcgccatgtg
      121 cgagtcggcg acttcgcgta tcttctccaa caccacgtcc tccagatagg cggcgttgat
      181 ccggtccacc tcggcgctca tccggaagac cgtccagttc tggtcgtcct ccaccgagag
      241 ctggtggagc gagaaaccca gcagcgaggc ggcatacccg aggatcgagc agactcttgc
      301 ggcggcgaag aacgggtcgt atatctcccg cgagaagagc agcagcgact gaccggccag
      361 catgacgccg atggagagtc ccagccacca cgtgatgctc tcgcccgaac gtcggaactc
      421 gcgggcatac actatcagcg cggctgtgaa cagggcggcg gagagaaagt ccagagggcg
      481 ggagagaagc gtgtggaacg ggagcggcga gccgttgagc gccgcgtaca gcgtgagcgc
      541 gatgatcagc ggcacggaga gcagtgcggg caggagttcc cgcccggggc gccggggcac
      601 gccctttccg gcggagatga cgagcggcgc catcaacagc aacggcgtca gcgtccggaa
      661 ggcgatcgaa ctccggagga cggcttcgcg aggcgcctgc gcgaagaacg gcccggcaag
      721 ttcaaggaaa ccgatcgcga ggtcctccgc tccggcgacg aagaaagcga gccccgcgaa
      781 caggtaaaag cgtctgccgg ggccggcgaa tctcgcgatg aaagcgcagc ccgccgaaag
      841 ggcgatcagc gcgcccgtca tcctgacgcc cccgaacacg ccggaatttc ctgcatgttc
      901 gaacgagcga agcagcggat acgccgaaaa cagcaggaga ggcgcaatga gcagcaggaa
      961 ttcgcgatac cggcttccgt tccacgagga gcggaatccc ttttccgcgg tctgtcccgt
     1021 cgggagactc ccgggtgttc gcgttgttgt tttcatgatg tcacccttcc gccccggcac
     1081 ggccggccgg cgttacagta tgccctccgc gccctcgttc gtcatgtggt cgcagatctg
     1141 ctcgaattcc tgctcgacgg cgaagaaggc gtcgacgacc gaagggtcga agtgcgtccc
     1201 gttcccctcc ctgatgatcc ggcaggccgt ttcgtgcggg aacgcctcct tgtacggccg
     1261 gtcggagcgg agcgcgtcgt agacgtccac gag
//
LOCUS       DAOTIL010000899         1292 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminobacteriaceae bacterium isolate AMR_MDS_3026
            NODE_125192_length_1292_cov_2.871463, whole genome shotgun
            sequence.
ACCESSION   DAOTIL010000899 DAOTIL010000000
VERSION     DAOTIL010000899.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868050
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminobacteriaceae bacterium (wastewater metagenome)
  ORGANISM  Aminobacteriaceae bacterium
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae.
REFERENCE   1  (bases 1 to 1292)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1292)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:19:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,224
            CDSs (total)                      :: 3,198
            Genes (coding)                    :: 3,184
            CDSs (with protein)               :: 3,184
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1292
                     /organism="Aminobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_125192_length_1292_cov_2.871463"
                     /isolate="AMR_MDS_3026"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:3034609"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1181)
                     /locus_tag="P5201_14335"
     CDS             complement(<1..1181)
                     /locus_tag="P5201_14335"
                     /inference="COORDINATES: protein motif:HMM:NF016232.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HRV99773.1"
                     /translation="MKRAALITADEGKTLEFKKDISSPRNLLKTLVAFANTAGGRIVI
                     GVEDKTRRPIGIDNPLDEEERLCNMIADSISPRLVPSIELTTVDGKTLLLVEVFLSSS
                     RPHYIVSEGPEAGVYVRLGSTNRQADRELIAELHRSVEGVSFDELPMPHLTVDDLAMN
                     SIKSSFEDIREVDEKTLLTLRLLIKEQGRNVPTKGGILLYGRDRPAHFPDCWAQCGRF
                     IGVDKTRIFDHIELEGPLPSLVDSIMLFLKKHAMRGADFSEVRRKDVWSIPIEPLREA
                     VVNAVVHADWSQRGAPVRVAFFDDRIEIENPGILPPGMTVEDMRAGVSKIRNPVIARV
                     FRELKLIEQWGSGIPRIFRETAAGGFPEPYFVELGLRLRFVFPLTKAIENTPKDYLVS
                     S"
BASE COUNT          288 a          368 c          351 g          285 t
ORIGIN      
        1 tccgaggaaa cgagataatc tttgggagta ttctcaatcg cctttgtcag cggaaaaaca
       61 aaacgcagcc gcagaccaag ttcgacgaaa tacggctcgg gaaaccctcc ggcagcagtt
      121 tcccggaaga tgcggggaat accggatccc cactgttcga tcagtttcaa ctcccgaaag
      181 acgcgcgcaa tcaccggatt gcgtattttg gaaactccgg cccgcatgtc ctcgacggtc
      241 attcccggag gcagaatgcc ggggttttcg atctcgatac ggtcgtcaaa gaaagcgaca
      301 cgtaccggag cgcccctctg ggaccagtcg gcatgcacca cggcgttgac aaccgcttcg
      361 cgcaaaggct caatgggtat gctccagaca tcctttcgac gcacttcgga aaaatccgcg
      421 ccgcgcatgg catgcttctt caggaagagc atgatggaat ccaccagcga cggcaacggg
      481 ccttcgagtt ctatatggtc gaatatccgg gttttatcga cgccgatgaa acggccgcac
      541 tgagcccagc agtcgggaaa gtgagcagga cgatctctac cgtaaagcag gatgccgcct
      601 ttggtcggta catttctccc ttgctctttg atcagcagtc tcagagtgag gagcgtcttt
      661 tcgtcgactt cgcgaatgtc ttcaaaggaa ctcttaatcg agttcatcgc aagatcgtcc
      721 acggtaaggt ggggcatggg caattcatcg aacgagactc cctcgactga acggtgaagc
      781 tcggctatca actcgcggtc cgcctggcgg ttcgtggagc ccaggcgaac gtatactcct
      841 gcctcgggac cttcggagac tatgtagtgc gggcgcgaac tgctcaagaa cacctcgacc
      901 agaagcagcg tcttcccgtc gacagtggtc agttctatac tggggaccaa acggggagat
      961 atggaatccg cgatcatgtt gcagagacgc tcctcctcgt ccagcggatt gtcgattcct
     1021 atcggtcgac gggttttgtc ctccaccccg atgacgatcc tgcccccggc tgtgtttgcg
     1081 aaagccacca gagtcttgag caggttccgg ggagaagaaa tatccttctt gaactccaga
     1141 gtcttgccct cgtcggctgt gattagtgca gctctcttca tatccccagc tcctcgaaat
     1201 gtatccgtat tcgcttgttg aagtcgcttt ccgccgcgtt catggtacac acaacgcggc
     1261 ggcgttgcaa gagcgcatac gattgtatcg cg
//
LOCUS       DAOTIL010000900         1289 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminobacteriaceae bacterium isolate AMR_MDS_3026
            NODE_125698_length_1289_cov_1.852512, whole genome shotgun
            sequence.
ACCESSION   DAOTIL010000900 DAOTIL010000000
VERSION     DAOTIL010000900.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868050
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminobacteriaceae bacterium (wastewater metagenome)
  ORGANISM  Aminobacteriaceae bacterium
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae.
REFERENCE   1  (bases 1 to 1289)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1289)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:19:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,224
            CDSs (total)                      :: 3,198
            Genes (coding)                    :: 3,184
            CDSs (with protein)               :: 3,184
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1289
                     /organism="Aminobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_125698_length_1289_cov_1.852512"
                     /isolate="AMR_MDS_3026"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:3034609"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..243
                     /locus_tag="P5201_14340"
     CDS             <1..243
                     /locus_tag="P5201_14340"
                     /inference="COORDINATES: protein motif:HMM:NF015722.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SUMF1/EgtB/PvdO family nonheme iron enzyme"
                     /protein_id="HRV99774.1"
                     /translation="GIYDMAGNVYEWCFDWYDVYYYQDSDASRNPRGPVSGKYRVYRG
                     GCLWRSDPSDFRGAYRIGDVPSYRDDSLGFRLVRTP"
     gene            474..1148
                     /locus_tag="P5201_14345"
     CDS             474..1148
                     /locus_tag="P5201_14345"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF012298.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HRV99775.1"
                     /translation="MPMPERLGKYRILRLLGAGAMGEVYLAEHELLRVLHAVKILPSG
                     VYGSQGFETRFREEARIMARLRHPGIVPVHDLDRDETTGAYFIAMDFVSPDGKSPQTL
                     DDLFEQSGGRLSHDRTGEILLALCDAVAYAHGEGVLHRDIKPSNILIDAGGTVRLADF
                     GLAEIVGQEYIEESLMENRTRGAGESAARSRAVKTPDSRQAARWAISSAVSHAATPRS
                     TCTRTQ"
BASE COUNT          232 a          397 c          426 g          234 t
ORIGIN      
        1 ggtatttatg atatggccgg aaacgtttat gagtggtgtt tcgactggta cgacgtgtac
       61 tactaccagg actcggatgc gtcgcggaat ccgaggggac ctgtgtcggg caagtaccgg
      121 gtgtaccgcg ggggttgctt gtggcgcagc gatccgtcgg acttccgggg agcgtaccgc
      181 atcggggacg ttccctccta ccgcgacgac tccctgggct tccgcctcgt gaggacgccc
      241 tagcccttgc cttcggccgg ttttccggtc gcggcagcgc cggggttctg ctttattttt
      301 ctgttttttc gatcggttgt tctcgaaggg gctcgtcgcg tgccttcagg cctgcgcgga
      361 atgtgcagcc tacctcggcg ggtgcctcgt cttcgaaaaa gcgcttcccg agacaagaaa
      421 tcgactgcat atagcaagga cgggtgcaac gaacacacaa ggaggtcatt ctcatgccga
      481 tgcccgaacg actgggcaag taccggatat taagactgct cggcgcgggg gctatggggg
      541 aggtctatct ggccgagcac gaactgcttc gcgtgctgca cgctgtcaag attcttccct
      601 cgggggtcta cggaagtcag ggttttgaaa cgcgcttccg cgaagaggct cgaataatgg
      661 ccagactgcg ccaccccgga atcgtccccg tgcacgacct cgacagagac gagaccaccg
      721 gcgcctactt catcgccatg gacttcgtct cgcccgacgg aaagagcccg cagacgctcg
      781 acgacctttt cgaacagagc ggaggacgcc tttcccacga cagaacgggg gagatactcc
      841 tcgccctctg cgacgccgtc gcctacgccc acggcgaagg agtgctccac cgcgacatca
      901 agccgtcgaa cattctgatc gacgccggag gaaccgttcg cctcgccgac ttcggactgg
      961 cggagatagt gggacaggag tacatcgaag agagcctcat ggagaaccgc acccggggag
     1021 ccggggagag cgcagcgcgc tcccgcgcgg tgaagacgcc cgactcgcgc caggccgcgc
     1081 gctgggcgat ctcgtcggcg gtgagccatg cggccacccc gcggtcgacg tgcacgcgca
     1141 cgcaataggc gcagccgttg agctgcgagg tgtggatgag cgcgatctct ttcagccggt
     1201 cgtcgatgcc ggcctcggcg gcgagggtgc cgaccgtctg cgcgaacgcc tcgagcgcgt
     1261 cgtacgcggg ccgggccgaa cgggacagg
//
LOCUS       DAOTIL010000901         1287 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminobacteriaceae bacterium isolate AMR_MDS_3026
            NODE_125897_length_1287_cov_3.599838, whole genome shotgun
            sequence.
ACCESSION   DAOTIL010000901 DAOTIL010000000
VERSION     DAOTIL010000901.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868050
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminobacteriaceae bacterium (wastewater metagenome)
  ORGANISM  Aminobacteriaceae bacterium
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae.
REFERENCE   1  (bases 1 to 1287)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1287)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:19:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,224
            CDSs (total)                      :: 3,198
            Genes (coding)                    :: 3,184
            CDSs (with protein)               :: 3,184
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1287
                     /organism="Aminobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_125897_length_1287_cov_3.599838"
                     /isolate="AMR_MDS_3026"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:3034609"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            269..>1287
                     /locus_tag="P5201_14350"
     CDS             269..>1287
                     /locus_tag="P5201_14350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014163861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_component: GO:0031317 - tripartite ATP-independent
                     periplasmic transporter complex [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_function: GO:0005488 - binding [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DctP family TRAP transporter solute-binding
                     subunit"
                     /protein_id="HRV99776.1"
                     /translation="MKKFALMFLVACLVLTSAAAFAETKTIKLAHLNPQQPFEVATAA
                     MTAVFKSMVEAESNGALKVDIFPAGQLGNERETMEQVKLGVVQTYIASAGGMAAFYPL
                     YSIVDIPFAIPNYDIAWKVYDGPFGQHLAKEIEAKAGFKVLGYGEAGGFFQLSNNTRA
                     IKTVADMKGLKMRTMTLPSHQNLMKAYGAAATPVAWAEVYTALQTGVVDGQHNPIPIV
                     LTGKLFEVQKYLTITNHLYSSYCWVMNKDFYEKLDEKERGIVDAAARTAIIAGRGLNR
                     IIESSEKGLPALIEAGMEINTPTPEALQEFREVGLASSMEFIKETYKDEGVALAQMYL
                     DAIEQA"
BASE COUNT          304 a          352 c          391 g          240 t
ORIGIN      
        1 tcacgcgatg gcggacaaga tcgacgaaaa aatcgaagaa ttcaacaaga tgacaggatt
       61 gctgtaaggt aaacgtccgc ccgaagtagt gattcgggga ggtgaggccg tttcatagag
      121 aaggagagtt cggcggctga cagaagctgt gaatacagtc gccggatgcg cagagccctg
      181 ccgtttcccg cgaaggggat ggagggtgga gttcagagag ttcccgttgt ttttttcggg
      241 agtcgttgac tgagaaggag gagacaaaat gaagaaattt gcattgatgt tccttgtagc
      301 gtgccttgtg ctgacatccg cggcagcgtt cgccgagacc aagacgatca agctggccca
      361 tctgaatccc cagcagccgt tcgaggtggc gacggcagcg atgaccgccg tatttaagag
      421 catggtcgaa gccgagagca acggcgctct caaggtcgac atcttccccg cagggcagct
      481 cggcaacgaa cgcgagacga tggagcaggt gaagctcggc gttgtccaga cctacatcgc
      541 ctcggcgggc ggcatggccg cgttctaccc cctttacagc atcgtcgaca tccccttcgc
      601 cattcccaac tatgacatcg catggaaggt atatgacggt cccttcggcc agcacctcgc
      661 gaaagagata gaggcgaagg ctggattcaa agttctcggc tacggcgagg cgggcggctt
      721 cttccagctc tcgaacaaca caagggcgat caagactgtg gcggatatga agggcctcaa
      781 gatgcggaca atgacgcttc cttcccacca gaacctcatg aaggcctacg gcgccgcggc
      841 cactcccgtt gcgtgggctg aggtgtacac cgcgctgcag acgggcgtcg tcgacggaca
      901 gcacaacccc attcccatcg tgctcaccgg aaagctcttt gaagttcaga agtacctcac
      961 catcaccaac cacctctaca gcagctactg ctgggtgatg aacaaggact tctacgaaaa
     1021 gctggatgag aaggaacggg gcatcgtcga tgcggcggcg cggaccgcga tcatcgccgg
     1081 acgaggcctg aaccggatca tcgagtcttc cgagaagggg cttcccgcgc ttatcgaggc
     1141 cgggatggag atcaacactc ccactcccga ggcgctgcag gagttccggg aggtaggtct
     1201 tgcttcttcg atggagttta tcaaggagac ttataaggat gaaggcgtcg cgctcgcaca
     1261 gatgtacctc gacgccatcg aacaggc
//
LOCUS       DAOTIL010000902         1286 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminobacteriaceae bacterium isolate AMR_MDS_3026
            NODE_126025_length_1286_cov_3.689683, whole genome shotgun
            sequence.
ACCESSION   DAOTIL010000902 DAOTIL010000000
VERSION     DAOTIL010000902.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868050
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminobacteriaceae bacterium (wastewater metagenome)
  ORGANISM  Aminobacteriaceae bacterium
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae.
REFERENCE   1  (bases 1 to 1286)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1286)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:19:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,224
            CDSs (total)                      :: 3,198
            Genes (coding)                    :: 3,184
            CDSs (with protein)               :: 3,184
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1286
                     /organism="Aminobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_126025_length_1286_cov_3.689683"
                     /isolate="AMR_MDS_3026"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:3034609"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..761)
                     /gene="rpoA"
                     /locus_tag="P5201_14355"
     CDS             complement(<1..761)
                     /gene="rpoA"
                     /locus_tag="P5201_14355"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013048057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000345 - cytosolic DNA-directed RNA
                     polymerase complex [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit alpha"
                     /protein_id="HRV99777.1"
                     /translation="MDYLRPEIRIEESTPKYAKVLIEPLERGYGATLGNSLRRVLLSS
                     IKGAAITSVRIDGVLHEFSTIPGVLEDVIEILLNLKHIPVRSFSQEVRVLRLETEGGG
                     LVTAANIQPDSEVEFVDPEAPICTMEEGARLSMDIYVEQGAGYASMERPRPAYLPVDA
                     LLVDAIFSPVLKVNYDVEAARVGQRTDFERLVLEIWTNGVHHPENTLCEASKILRGYF
                     GNIVEDMEKLNPVEPREQEAAAAGEGGVSEAAQER"
     gene            complement(785..>1286)
                     /gene="rpsD"
                     /locus_tag="P5201_14360"
     CDS             complement(785..>1286)
                     /gene="rpsD"
                     /locus_tag="P5201_14360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013048056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="30S ribosomal protein S4"
                     /protein_id="HRV99778.1"
                     /translation="KGRPRTKVSEYGIRLREKQKLRRFYGMTELQFKRFFEIANKQTG
                     QTGHNFLSLLERRLDNVVYRLGFANSRKQARQLVLHGHFRVNGKRVDIPSYLVESGDL
                     VAVMEKSREVTLIKENAEVATARKAPEWLEVNAEKIEGKVLSLPSREQIDAPVTEQLV
                     VEFYAR"
BASE COUNT          248 a          395 c          352 g          291 t
ORIGIN      
        1 atacgctcct gggctgcctc ggagacgccg ccctcgccgg ctgctgccgc ctcctgctcc
       61 ctgggttcca cggggttgag tttctccatg tcctccacga tattgccgaa gtaccctctc
      121 aggattttcg acgcttcgca gagcgtattc tcgggatgat gcaccccgtt cgtccagatc
      181 tcaaggacaa ggcgttcaaa gtccgtgcgc tggccgacgc gcgccgcctc cacgtcgtag
      241 tttactttga ggacagggga gaagatcgcg tcgaccaaca gggcgtccac agggagatag
      301 gccgggcgcg gacgctccat ggaggcgtac ccggcgccct gttcaacgta gatatccatc
      361 gagagacggg cgccctcctc catcgtgcag atcggggctt ccgggtcgac aaactccact
      421 tcgctgtccg gctgtatgtt cgcggcggtg acaagacctc cgccttccgt ctccagccga
      481 agcactcgga cttcctggct gaaagagcgg acgggtatat gcttcagatt cagcaaaatt
      541 tcgatgacat cttccagaac accgggtatg gtgctgaatt catggagcac gccgtcgatt
      601 cgaacggacg tgatggccgc acccttgatc gaggagagta gaactcttct gagagaattt
      661 cccagagtcg ctccatatcc ccgctcgagc ggttcaatga gaaccttcgc atacttaggg
      721 gtgctttcct cgatacggat ctcaggccgc aagtaatcca aggcttccaa cacctttcac
      781 gccactatct ggcatagaac tcgacgacga gctgctcggt caccggcgca tcgatctgct
      841 cacggctggg gagcgaaaga accttccctt cgattttttc tgcgttgacc tcaagccact
      901 cgggcgcctt ccgcgcggtc gctacttccg cattttcctt gatcagcgtg acttcgcggc
      961 tcttttccat cacggcgaca agatcgccgc tttcgacgag atagctgggg atgtccacac
     1021 gcttcccgtt cacacggaaa tgaccgtgaa gcacaagctg tctggcctgc tttctgctgt
     1081 tggcgaatcc gaggcgatac acaacattgt ccagtctgcg ctcaagcagg ctgaggaagt
     1141 tgtggcccgt ctgccccgtc tgcttgttgg ctatctcgaa gaagcgcttg aactgcaact
     1201 cggtcatgcc gtagaagcgg cgaagcttct gtttctcacg aagacgaatg ccgtactcgc
     1261 tgaccttggt acggggacgt cccttt
//
LOCUS       DAOTIL010000903         1285 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminobacteriaceae bacterium isolate AMR_MDS_3026
            NODE_126221_length_1285_cov_2.717886, whole genome shotgun
            sequence.
ACCESSION   DAOTIL010000903 DAOTIL010000000
VERSION     DAOTIL010000903.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868050
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminobacteriaceae bacterium (wastewater metagenome)
  ORGANISM  Aminobacteriaceae bacterium
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae.
REFERENCE   1  (bases 1 to 1285)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1285)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:19:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,224
            CDSs (total)                      :: 3,198
            Genes (coding)                    :: 3,184
            CDSs (with protein)               :: 3,184
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1285
                     /organism="Aminobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_126221_length_1285_cov_2.717886"
                     /isolate="AMR_MDS_3026"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:3034609"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(244..>1285)
                     /locus_tag="P5201_14365"
     CDS             complement(244..>1285)
                     /locus_tag="P5201_14365"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRV99779.1"
                     /translation="QQNKTAVALANDPSVPAALRAKLNKTLQEINRRVDKNTARGIVN
                     KVRSVNKKVEDFVRTELPQAGSKAGTKIDQFLRDNPGLTREDVLFANRVESFLRKNPG
                     MRREDILVRSRTVSGVDPTKLGRDRDVYFQFVDRRGKVLGDVHHDIAAPIYNQKLQAA
                     TGLSSKQLDHTVTSTWHPDSYNPGRMASDGPRRQLVDDIVKGRAAGKLARPQDIRGTV
                     IGKATERFDDARRLEAAGNPSAAAEAYAEGMRQLGKDYERHVAPFLRNQNLDPAAALP
                     PRLKAALDIFKKVEQGTTSGAYTPEQGLRALQSLSCRTPGGGNIPMSPDKAAEDLGLF
                     IEMMNKWMIQGR"
BASE COUNT          200 a          429 c          362 g          294 t
ORIGIN      
        1 atcaacaccc ccacgacgag aaataccgat cctgcgaacg aaaagtcttt ttccgccgcg
       61 aagatcagcg cagcgacaaa aagctgaaag cctcctacgg cgatcttgta gcgaattttc
      121 atatcaacgt ctccttttcg tgccttccgc acagtgcgga gagagggaag gcgcaggcct
      181 cccgcccgtt ccttccctct ctcttcatcc gcttatgcgc tcatgttttt ctgcgaacgc
      241 tccttaccgc ccttggatca tccacttgtt catcatctcg atgaagaggc ccaggtcctc
      301 cgccgccttg tcgggcgaca tcgggatgtt gcctcctccg ggggttctgc acgacagcga
      361 ctgaagggcg cggagcccct gctccggcgt ataggctccc gacgtggtcc cctgctccac
      421 cttcttgaag atgtcgagcg cggccttcag tctggggggc agcgccgccg caggatcgag
      481 gttctggttc cgcaggaagg gggccacgtg acgctcatag tccttgccca actggcgcat
      541 tccttcagcg tacgcctcgg ccgccgcgct cgggttcccc gcggcttcaa ggcgtcttgc
      601 gtcgtcgaac ctctccgtgg ccttcccaat gacggtgccg cggatatcct gcgggcgggc
      661 cagctttccc gccgccctgc ccttcacgat atcgtcgacg agctgtctgc gcggtccgtc
      721 cgaagccatt cttcccggat tgtacgagtc cgggtgccac gtgctggtga cggtgtggtc
      781 gagctgcttc gacgaaagcc ccgttgcggc ctggagcttc tgattgtaaa taggcgctgc
      841 gatatcgtga tggacgtcgc cgaggacctt gcctcttcgg tccacgaact ggaaatagac
      901 gtccctgtcg cggccgagct tcgtcggatc gactccggaa accgtcctgg agcgaacaag
      961 aatatcttct ctcctcatac cggggttttt cctgaggaac gactccaccc tgttcgcgaa
     1021 gagcacgtcc tctctggtca gccccggatt gtccctgagg aactggtcta tcttcgtgcc
     1081 cgccttcgac ccggcctgcg gcagttcggt gcgcacgaag tcctccacct tcttgttgac
     1141 cgaccgcacc ttgttcacga tcccccgcgc ggtgttcttg tcgacgcgcc ggttgatctc
     1201 ctgcagcgtc ttgttcaatt tcgccctgag ggccgcgggc acgctcggat cgttggcgag
     1261 cgccacggcg gtcttgttct gctgc
//
LOCUS       DAOTIL010000904         1284 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminobacteriaceae bacterium isolate AMR_MDS_3026
            NODE_126340_length_1284_cov_3.479251, whole genome shotgun
            sequence.
ACCESSION   DAOTIL010000904 DAOTIL010000000
VERSION     DAOTIL010000904.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868050
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminobacteriaceae bacterium (wastewater metagenome)
  ORGANISM  Aminobacteriaceae bacterium
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae.
REFERENCE   1  (bases 1 to 1284)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1284)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:19:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,224
            CDSs (total)                      :: 3,198
            Genes (coding)                    :: 3,184
            CDSs (with protein)               :: 3,184
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1284
                     /organism="Aminobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_126340_length_1284_cov_3.479251"
                     /isolate="AMR_MDS_3026"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:3034609"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            94..648
                     /locus_tag="P5201_14370"
     CDS             94..648
                     /locus_tag="P5201_14370"
                     /inference="COORDINATES: protein motif:HMM:NF018979.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter TctB
                     family protein"
                     /protein_id="HRV99780.1"
                     /translation="METLLLFLSGADSFPVWILSFLAVIALTAFLTRGKHAPFRGRII
                     ICVSLLFLAFAFFLLSFGLEEIEAVGSSARTAPRLWAAGLAFFTLDQLRRTLLGKAPE
                     DPKTGDVQRVLLAAAIVAFSLWGMEIVGYLISSGGMILLLLLLFGERRLFILLSLTGG
                     WMLFSWFVFIRLLHLSLPAGIYFG"
     gene            661..>1284
                     /locus_tag="P5201_14375"
     CDS             661..>1284
                     /locus_tag="P5201_14375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009166023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter permease"
                     /protein_id="HRV99781.1"
                     /translation="MDLFSHLLDGLGALMGITPLLVIMAGVAIGILAGAMPGISPSMA
                     VALLLPFSFGMTPSMGLVLLCAIYLASNYGGSITAVMVNTPGTPSAVVTAFDGYPLTR
                     KGDAGTALGVSLVASVAGGFIGIIILVLFSAPLARMALKFWPAEYFALAILGLSTIAS
                     LGGGRWLESFMAVLLGLLLNTIGLDPLSGVSRFTFDVVRLYDGFSFIP"
BASE COUNT          177 a          373 c          369 g          365 t
ORIGIN      
        1 gacggcggtt ccccgcactt catccgccgg tagtacggcg ggcttggtgg cgtacaataa
       61 caatggagcc ggggtgctcc cggaggtggt tccatggaaa ctctgcttct ttttctcagc
      121 ggggccgaca gtttccccgt ctggattctt tcgtttcttg ccgtcatagc gctgacggcc
      181 ttcctgacac gtgggaagca cgcgccgttc agggggcgta taatcatctg cgtgtcgttg
      241 cttttccttg cattcgcttt ctttcttctc tcattcggcc tcgaggagat cgaagccgtc
      301 ggaagcagcg ccagaacggc gcccaggctc tgggctgcag gactggcttt ttttactctg
      361 gatcagctgc gacgcacgct cctggggaag gctcctgagg atccgaagac cggtgacgta
      421 caacgggttc tgctggctgc ggccattgtc gctttctctc tttgggggat ggagatcgtc
      481 ggttacctga tctcctcggg cggaatgatt cttctccttc tgctgctctt tggagaacgc
      541 cggttgttta tccttctttc actgacaggg ggctggatgc tcttctcctg gtttgtcttt
      601 attcgcctgc tgcatctctc gcttcctgcg ggcatctatt tcggataggg ggaacgtcga
      661 atggatcttt tctcccattt gcttgacgga ctcggagcgc tcatgggaat tactcctttg
      721 cttgtaatta tggcgggcgt ggcgatcggc attcttgcag gcgccatgcc cggcatctct
      781 ccctccatgg ccgtcgcgtt gcttctgcca ttttcctttg gaatgacccc ctcgatgggg
      841 ctcgtcctgc tttgcgctat ctaccttgcc tccaactacg gggggtcgat caccgccgtc
      901 atggtgaaca cgccggggac gccctcggct gtggttaccg cctttgacgg ctatcctctg
      961 acacgaaaag gcgatgcggg aaccgcgctc ggcgtttctc ttgtcgcttc tgttgccgga
     1021 ggatttatcg gaatcatcat cctggtgctc ttctcggccc ccttggcgcg tatggcgctg
     1081 aagttctggc ctgcggagta ctttgctctg gcaattctcg gactctccac gatagcctct
     1141 ttgggaggag gacgctggct tgagtccttc atggcggtgc ttctggggct gctcctgaat
     1201 accatcgggc tcgaccctct cagcggtgta agccgcttta cgttcgacgt ggtccgcttg
     1261 tacgacggtt tctccttcat tccg
//
LOCUS       DAOTIL010000905         1280 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminobacteriaceae bacterium isolate AMR_MDS_3026
            NODE_126923_length_1280_cov_3.364082, whole genome shotgun
            sequence.
ACCESSION   DAOTIL010000905 DAOTIL010000000
VERSION     DAOTIL010000905.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868050
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminobacteriaceae bacterium (wastewater metagenome)
  ORGANISM  Aminobacteriaceae bacterium
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae.
REFERENCE   1  (bases 1 to 1280)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1280)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:19:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,224
            CDSs (total)                      :: 3,198
            Genes (coding)                    :: 3,184
            CDSs (with protein)               :: 3,184
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1280
                     /organism="Aminobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_126923_length_1280_cov_3.364082"
                     /isolate="AMR_MDS_3026"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:3034609"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>1280
                     /locus_tag="P5201_14380"
     CDS             <1..>1280
                     /locus_tag="P5201_14380"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRV99782.1"
                     /translation="KLVQKMMHLAVVKIGGKVLSRMIPIIGVGLLLYEGIQMGQAKEA
                     FEEELRKSFFTEYTADVTVESVWNASPDGTLPSMKQEMERNINTLLSDWERICRSEAV
                     SMIHSAQVLASSEHVRNYVSGELEKGADFQLLLQKINALWDAFGQSLALEPVEFFESI
                     LIDSPDRNDLRLLSRDPGSRFIELYKEWGAEFLKAVNKIGVENYVSTEWKTSDVNWSV
                     LNRSLDFLPDISGNRNAARGLLFLIQEGVPLDGFSPDLMAKIAVKKDVFLKVWQALTP
                     DSRKLAVIFESGRPLVILEPSLIAFPEACSLFLRDYGVEFWTSWSSDDIFDLLRISEI
                     RGKNSGKLRAASVLPEERADLLEIYRRSGERGIILWDIHTKEPTGEIGRKLARQSIAC
                     MAEGYPFEDMKDPEFVRFAAATLNFPAGRFFYDT"
BASE COUNT          285 a          350 c          377 g          268 t
ORIGIN      
        1 gaagctggtc cagaaaatga tgcacctggc ggtggtaaag atcggaggga aagtgctctc
       61 gcggatgatc cccatcatcg gggtcggcct gcttctgtac gagggcatcc agatgggaca
      121 ggcgaaagaa gcgttcgaag aggagctcag gaagagcttt tttacggagt acactgccga
      181 cgtcaccgtg gagtccgtct ggaacgcgag tcccgatgga acgctcccct ctatgaaaca
      241 ggagatggaa cggaatatca acacgctgct gagcgactgg gagcggatct gccgttccga
      301 ggccgtctcg atgattcaca gcgcgcaggt gctcgcttcc tcggaacatg tcaggaacta
      361 cgtaagcgga gaactcgaga agggcgccga cttccagctg ctgcttcaga agatcaacgc
      421 cctttgggat gcgttcggac agtcgctcgc cctggagccc gtcgaatttt tcgagtcgat
      481 tctcattgat tcccccgaca gaaacgatct gcgtctgctc tcgcgtgacc caggcagcag
      541 gtttatcgag ctgtacaaag agtggggagc ggaatttctg aaagcggtca acaagatcgg
      601 ggtggagaac tacgtatcca ccgagtggaa gacgagcgac gtcaactgga gcgtactgaa
      661 cagaagcctt gattttcttc cggacatcag cggcaaccgg aacgccgcgc gcggcctgct
      721 ctttttgatc caggaggggg ttcctctcga cggcttttct ccggacctga tggcaaagat
      781 cgccgtaaag aaggatgtct tcctgaaagt atggcaggcg ctcactcctg actccagaaa
      841 gctcgcggtc atcttcgaga gcggacgtcc cctcgttatt cttgagccgt ccctcatcgc
      901 ctttcccgaa gcctgctcgc tcttccttcg cgactacggg gtggagttct ggacctcctg
      961 gtcgagcgac gatatcttcg atctcctcag gatttcggaa attcgcggaa agaacagcgg
     1021 gaaactacga gcggcttccg tccttccgga ggaacgcgcg gatctgctcg aaatctaccg
     1081 ccgttccggg gagaggggaa ttattctgtg ggacatccat acgaaggaac caacaggaga
     1141 aatcggcaga aagctcgccc gtcagagtat cgcctgcatg gccgaggggt accccttcga
     1201 ggacatgaag gatccggagt ttgtccgttt cgccgcagcc acacttaact ttccggcagg
     1261 gcgttttttc tacgacacac
//
LOCUS       DAOTIL010000906         1280 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminobacteriaceae bacterium isolate AMR_MDS_3026
            NODE_126939_length_1280_cov_3.024490, whole genome shotgun
            sequence.
ACCESSION   DAOTIL010000906 DAOTIL010000000
VERSION     DAOTIL010000906.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868050
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminobacteriaceae bacterium (wastewater metagenome)
  ORGANISM  Aminobacteriaceae bacterium
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae.
REFERENCE   1  (bases 1 to 1280)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1280)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:19:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,224
            CDSs (total)                      :: 3,198
            Genes (coding)                    :: 3,184
            CDSs (with protein)               :: 3,184
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1280
                     /organism="Aminobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_126939_length_1280_cov_3.024490"
                     /isolate="AMR_MDS_3026"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:3034609"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..808
                     /locus_tag="P5201_14385"
     CDS             <1..808
                     /locus_tag="P5201_14385"
                     /inference="COORDINATES: protein motif:HMM:NF025714.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF4403 family protein"
                     /protein_id="HRV99783.1"
                     /translation="TVLGSSPELWLAVTPLSVSMPPFSAKDGRLTATAGIKCMLKITA
                     EKPDPLRASSLPALAAMPPHMEPGILLNVESTLSYASLGKYVSSMDFPEVDIPGGGRV
                     KVKEVSFFGSGDKLVAAADISGKGPVGGAIEGKVYIMGRPAYSREDETIRMEDADFEE
                     QTNSALAKAAAWLARPALLKEMTERMVFPLQELREQTVRSLAELLREQKIAEDVLMEG
                     SVSALSLESLAVGDNGLRLSLSLGGAVSLRYEDESLKRKEKEAKNGGKKN"
     gene            896..>1280
                     /locus_tag="P5201_14390"
     CDS             896..>1280
                     /locus_tag="P5201_14390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015557192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HRV99784.1"
                     /translation="MKRIGLFCVFVLLCALFAGAAFASEKVSAYATTEEPLAKELFDA
                     FEKETGIKVDWVRLSSGEAVARLEAEKANPRASIWVGGVGTLHIDAKNKGLTAPYNSR
                     AASSIPDKFKDPERFWMGLYVGPIAF"
BASE COUNT          270 a          401 c          378 g          231 t
ORIGIN      
        1 cacagttctg gggtcgtccc ccgaactgtg gctcgccgtc acgccgctct ccgtctccat
       61 gccgccgttc tcggcgaagg acggccgcct cacggcgacc gcagggatca aatgcatgct
      121 gaaaatcacc gccgagaagc cggacccgct cagggcatct tcgctccccg cgctcgcggc
      181 gatgccgccg cacatggagc cgggcattct gctcaatgta gaaagcacgc tctcctatgc
      241 atctctgggc aagtacgtct cctcgatgga ctttcccgag gtcgacattc ccggcggggg
      301 gcgcgtcaag gtgaaggagg tctctttctt cggcagcggc gacaaactcg tcgccgccgc
      361 cgatatctcg ggaaaaggcc ccgtgggagg cgccatcgag ggaaaggtct acatcatggg
      421 gcggcccgcc tattcacggg aggacgaaac gatccggatg gaagacgccg acttcgagga
      481 acagacgaac agcgcccttg caaaggccgc ggcgtggctc gcccgccccg cgcttctcaa
      541 agagatgacc gaacgaatgg tcttcccgct ccaggagttg agggaacaga ccgtacggtc
      601 gctcgcggaa ctgctgcggg aacagaaaat tgcggaggac gtcctcatgg agggctccgt
      661 ctccgcgctc tctcttgaaa gcctcgcggt aggcgataac gggctccgcc tctccctctc
      721 cctcggaggc gccgtttcgc ttcgctacga ggacgagtca ttgaaacgga aggaaaaaga
      781 ggcgaagaat ggcggtaaaa agaattgaca caacgcctct cactccatat actcaaaaag
      841 gttttataac ggttccggaa agtgaacctc tattacagca aagggagatg aaagtatgaa
      901 acgaatcggt ctgttctgtg tttttgttct gctctgcgcg ctcttcgccg gcgcggcgtt
      961 cgcctcagaa aaggtgagcg cctacgccac aacggaagaa cccctcgcca aagaactttt
     1021 cgacgccttc gagaaggaaa ccggcatcaa ggtcgactgg gtccgtctct cctccggcga
     1081 ggccgttgca cgcctcgagg ccgaaaaggc caaccccagg gcgtccatct gggtcggcgg
     1141 cgtgggcacg ctccacatcg acgcgaagaa caaggggctc accgcgccgt acaactcccg
     1201 cgcggcctcc tctattcctg ataagttcaa agaccccgag cgtttctgga tggggctcta
     1261 cgtcggcccg atcgcgttcg
//
LOCUS       DAOTIL010000907         1278 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminobacteriaceae bacterium isolate AMR_MDS_3026
            NODE_127225_length_1278_cov_3.426002, whole genome shotgun
            sequence.
ACCESSION   DAOTIL010000907 DAOTIL010000000
VERSION     DAOTIL010000907.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868050
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminobacteriaceae bacterium (wastewater metagenome)
  ORGANISM  Aminobacteriaceae bacterium
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae.
REFERENCE   1  (bases 1 to 1278)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1278)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:19:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,224
            CDSs (total)                      :: 3,198
            Genes (coding)                    :: 3,184
            CDSs (with protein)               :: 3,184
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1278
                     /organism="Aminobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_127225_length_1278_cov_3.426002"
                     /isolate="AMR_MDS_3026"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:3034609"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..681)
                     /locus_tag="P5201_14395"
     CDS             complement(<1..681)
                     /locus_tag="P5201_14395"
                     /inference="COORDINATES: protein motif:HMM:NF015321.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase large subunit"
                     /protein_id="HRV99785.1"
                     /translation="MKPSNYDVVLDYAKSIVDGRKIACKEKRQECERFFRDLDNPAYE
                     FDCKDPEFVIRFIETCVSHKEGESVQGEPLLGKPLLLEPWEKFIVYNLLGFRLKGTRE
                     RRFKEAFLFIARKNGKTPFAAALALALAFLERRSGSRIYIVGAALKQARQAFDHILFN
                     LDQMGELREFRVLDNNAEHSIERTFFDHGKVTGSLRIEALAANPDKQDSLVANIQICD
                     ELHAYKSAK"
     gene            complement(668..1024)
                     /locus_tag="P5201_14400"
     CDS             complement(668..1024)
                     /locus_tag="P5201_14400"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRV99786.1"
                     /translation="MARPQKTWTNRDFEQFEALCKIQCSKADICAVMDVSEKTLDRLV
                     KEQYGKTVEDMQDHFRAYGRASLLRSQFKLAERNPSMAIWLGKQYLGQQDPNMRRPPG
                     EGRPPSRLGQFLDETE"
BASE COUNT          212 a          386 c          362 g          318 t
ORIGIN      
        1 cttggcgctc ttgtaagcgt gcagctcgtc gcagatctgg atgttcgcca ccagcgaatc
       61 ctgcttgtcg gggtttgccg cgagcgcttc gatgcgcagg gagcctgtca cttttccatg
      121 gtcaaagaac gtccgctcga tcgagtgctc ggcgttgttg tccagcacac ggaactccct
      181 gagctctccc atctggtcga ggttgaacag gatgtggtcg aacgcctgcc gggcctgctt
      241 gagcgccgcg ccgactatgt agatgcggga tccgctcctg cgttcgagga acgccaacgc
      301 cagtgccagc gccgcggcga acggggtctt gccgttcttc cgggcaataa aaaggaacgc
      361 ctctttgaag cggcgttccc tagtgccttt gagccgaaat cccaggaggt tgtagacgat
      421 gaacttctcc cacggttcga gcagcagcgg ttttcccaag agcggttcgc cctgtacgga
      481 ttcgccttcc ttgtgcgaga cgcatgtttc gatgaaccgt attacgaact ccggatcttt
      541 gcagtcgaac tcgtacgccg ggttgtccag atcccgaaag aaccgctcgc actcctgccg
      601 cttttcctta cacgcgatct ttctgccgtc gacgatgctc ttggcgtaat ccaatacgac
      661 gtcgtagtta ctcggtttca tctaaaaact gtccgagccg cgacggaggt ctgccttcgc
      721 cgggcggccg tctcatgttg ggatcctgct gtccaaggta ctgcttcccc agccagatcg
      781 ccatgctggg attccgctcg gcgagcttga actgcgagcg caagagcgac gctctgccgt
      841 atgcgcgaaa gtggtcctgc atatcctcca ccgtcttgcc gtattgttcc ttgacaaggc
      901 ggtcgagcgt cttttccgaa acgtccatga cggcgcaaat atccgccttc gagcactgga
      961 ttttgcagag ggcttcgaac tgctcaaagt ccctgtttgt ccatgttttt tggggtcttg
     1021 ccatgtcgtt tcacctgcgc ctttccgggg ggtttttccc tatacccccc tctgtacttc
     1081 gcgcacactt ggaagagggg aaggcccctg gtcgttaagc ctgcgtccgc gtttgccctg
     1141 accggggggg atcagtgcct tccgcccttc tccgggtgac gcttactgtg gcaagcgtta
     1201 cagagcgcta tcccgttgct catgctgtac gcaaggtcgg gtcgctcgct gatcggctgt
     1261 atatggtgcg ctacctct
//
LOCUS       DAOTIL010000908         1277 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminobacteriaceae bacterium isolate AMR_MDS_3026
            NODE_127408_length_1277_cov_2.950082, whole genome shotgun
            sequence.
ACCESSION   DAOTIL010000908 DAOTIL010000000
VERSION     DAOTIL010000908.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868050
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminobacteriaceae bacterium (wastewater metagenome)
  ORGANISM  Aminobacteriaceae bacterium
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae.
REFERENCE   1  (bases 1 to 1277)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1277)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:19:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,224
            CDSs (total)                      :: 3,198
            Genes (coding)                    :: 3,184
            CDSs (with protein)               :: 3,184
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1277
                     /organism="Aminobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_127408_length_1277_cov_2.950082"
                     /isolate="AMR_MDS_3026"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:3034609"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..592
                     /locus_tag="P5201_14405"
     CDS             <1..592
                     /locus_tag="P5201_14405"
                     /EC_number="2.4.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013048552.1"
                     /note="Catalyzes first step of the de novo purine
                     nucleotide biosynthetic pathway; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="amidophosphoribosyltransferase"
                     /protein_id="HRV99787.1"
                     /translation="VRKELGKKLAKRAPCREASLVTGMPDSGTIASLGYAEESGVPYE
                     QSVVRNRYVGRTFIEPTSRVRHLGVRIKLNPITEVLQGKNVVVVDDSIVRGTTCERMI
                     SMIKSCGANQVHVRISSPPVRFPCYYGIDTPTRVELAAARMNLQQLEQEVGADSLAYI
                     TEEDLLEAIGLPAQSVCTACFSGDYMEGGEEYEPQL"
     gene            576..>1277
                     /gene="purM"
                     /locus_tag="P5201_14410"
     CDS             576..>1277
                     /gene="purM"
                     /locus_tag="P5201_14410"
                     /EC_number="6.3.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008523254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004641 -
                     phosphoribosylformylglycinamidine cyclo-ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine cyclo-ligase"
                     /protein_id="HRV99788.1"
                     /translation="MNLSYKNAGVDISLADQWVDVIKGISKSMPAKKEIIGGIGGFSG
                     LYRLPGGMALAGCCDGVGTKIEIARAAGMFTHIGQDLVAMNVNDLITCGARPLFFLDY
                     IACGKLNIPVLQPIVESVARACGECGCALLGGETAEMPGVYPEDGLDLAGFAVGIVEE
                     EKIIDGKNVAAGDAVVGIPSSGVHSNGFSLVRRALFSEGTPYSLDEAMPRFGGATLAE
                     ILLAPTKLYVNTALRA"
BASE COUNT          278 a          359 c          384 g          256 t
ORIGIN      
        1 ggtacgtaag gaactgggga aaaagcttgc gaaaagagcg ccttgccggg aagcctctct
       61 ggtcaccgga atgcccgaca gcggaacgat cgcttctctc ggctacgcgg aagagagcgg
      121 cgtaccctac gagcaaagcg ttgtccgcaa ccggtacgtg ggacggacct tcatcgaacc
      181 gacaagcagg gtgcggcatc ttggtgtgcg gattaaactg aatcccatca ccgaggtgct
      241 ccaagggaag aacgtcgtcg tcgtcgacga ctcgatagtg cgcgggacga catgcgaacg
      301 gatgatctcc atgatcaagt cgtgcggcgc gaaccaggtc catgtacgta tctcctctcc
      361 tccggtgcgc ttcccttgct actatgggat cgacaccccc acgcgggtgg aactggcagc
      421 cgcgcgcatg aatcttcaac agcttgagca ggaggtcggc gccgattcgc tggcgtacat
      481 caccgaagag gatcttctgg aagctattgg ccttcccgcg cagagcgtct gcacagcctg
      541 tttttcggga gattacatgg aaggcggcga agaatatgaa cctcagctat aaaaacgcgg
      601 gagtcgatat ctccctcgcg gatcagtggg tcgacgttat caagggaatt tccaaatcaa
      661 tgcctgcaaa gaaggagatc atcggaggca taggggggtt cagcggtctg tacagactcc
      721 ctggaggaat ggctctagcc ggctgttgcg acggcgtagg cacaaaaatc gaaattgcca
      781 gggccgcggg aatgtttacg catatcgggc aggatcttgt cgccatgaac gtcaacgatc
      841 tgatcacctg cggagcccgt ccgctcttct ttcttgacta catcgcctgc gggaaactga
      901 atatccccgt tcttcaaccg atcgtcgaaa gtgtcgccag ggcgtgcggc gagtgcgggt
      961 gcgcactgct tggcggtgaa accgccgaga tgccgggcgt ctaccccgag gatggactcg
     1021 acctcgccgg gtttgccgtc ggcatcgtcg aagaagaaaa gataatcgac gggaaaaatg
     1081 ttgcggcggg agacgccgtc gtcggtattc ccagctccgg cgttcacagc aacggttttt
     1141 ccctggttcg ccgcgctctc ttttctgaag gtacgccgta cagccttgac gaagcgatgc
     1201 cgcgcttcgg aggcgccacg cttgcggaaa ttctccttgc tccgacaaag ctctacgtca
     1261 acacagccct tcgggcc
//
LOCUS       DAOTIL010000909         1277 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminobacteriaceae bacterium isolate AMR_MDS_3026
            NODE_127446_length_1277_cov_2.397709, whole genome shotgun
            sequence.
ACCESSION   DAOTIL010000909 DAOTIL010000000
VERSION     DAOTIL010000909.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868050
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminobacteriaceae bacterium (wastewater metagenome)
  ORGANISM  Aminobacteriaceae bacterium
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae.
REFERENCE   1  (bases 1 to 1277)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1277)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:19:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,224
            CDSs (total)                      :: 3,198
            Genes (coding)                    :: 3,184
            CDSs (with protein)               :: 3,184
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1277
                     /organism="Aminobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_127446_length_1277_cov_2.397709"
                     /isolate="AMR_MDS_3026"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:3034609"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..441
                     /locus_tag="P5201_14415"
     CDS             <1..441
                     /locus_tag="P5201_14415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005660842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA ester lyase"
                     /protein_id="HRV99789.1"
                     /translation="GLTIGGQDLAADLGIKATREGLEMLYAKSAVILAAKANGLLAFD
                     TVYTDINNQEGLREQAKMSVSLGFSGKAAIHPSQIAIIHEAFSPDEKEVRKAERIVRG
                     AKEAEERGLGVVAVDGRMVDAPVVAQAVRTLELARLAGMEVEIL"
     gene            438..>1277
                     /locus_tag="P5201_14420"
     CDS             438..>1277
                     /locus_tag="P5201_14420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014163811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_component: GO:0009346 - ATP-independent citrate lyase
                     complex [Evidence IEA];
                     GO_function: GO:0008814 - citrate CoA-transferase activity
                     [Evidence IEA];
                     GO_process: GO:0006084 - acetyl-CoA metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="citrate lyase subunit alpha"
                     /protein_id="HRV99790.1"
                     /translation="MKPENLVKNAIGREVPTSVQGLGEFTPYAGPRARLDGKHLWTPT
                     LYPRKIAAPTTNKVAHSLKEAIEKSGLRDGMTISFHHHLRNGDALVGMTLSLLDEMGI
                     RNITLAPSSLGDSHDCVADAVRKGVVTRLNTSGVRGEVGKAISNGEFELPVMIRSHGG
                     RARAVEEGSLHIDVAFLAAPACDRLGNFTGSAGKTACGSMGYAMLDARYADHVIAVTD
                     NLAEYPLAPRISVPQYLVDQVVVVDSIGDPSKIATGATRVTRDPVQLRIAELAFGLLK
                     ASGM"
BASE COUNT          250 a          415 c          415 g          197 t
ORIGIN      
        1 ggcctcacca tcggcgggca ggatctcgcc gccgacctcg gcatcaaggc gacccgcgaa
       61 gggctggaga tgctctacgc aaagagcgcc gtcatcctcg ccgcgaaggc gaacggtctg
      121 ctcgcgttcg atacggtcta caccgacatc aataaccagg aaggactccg cgaacaggca
      181 aagatgtcgg tctctctggg cttctccggc aaggccgcca ttcacccctc gcagatcgcg
      241 atcattcacg aagcgttctc cccggacgag aaagaggtcc ggaaggccga gcgcatcgtc
      301 cggggggcaa aggaagcaga agagcgcgga ctgggcgtcg tggccgtcga cggccgcatg
      361 gtggacgcgc ccgtcgtcgc gcaggctgtg cggacgctcg aacttgcccg tctcgcggga
      421 atggaggtcg agatcctatg aaaccggaga atcttgtgaa gaacgccatc ggaagagagg
      481 tcccgacgtc cgttcagggg ctcggagagt tcactcccta cgcgggcccg cgcgcacggc
      541 tcgacgggaa gcacctctgg acgccgacgc tctaccccag aaaaatcgcc gcgccgacga
      601 cgaacaaggt ggcgcacagc ctcaaggaag cgatcgagaa gtcgggactc cgcgacggca
      661 tgacgatctc cttccatcat cacttgagga acggcgacgc tctcgtcggg atgacactct
      721 cgctgctcga cgagatgggc atacggaaca tcacgctcgc tccgagttcg ctgggcgact
      781 cgcacgactg cgtcgccgac gccgtgcgta agggagtcgt cacgcgcctg aacacatctg
      841 gcgtccgtgg agaggtcggc aaagcaatat cgaacggcga gttcgagctt cccgtgatga
      901 ttcgcagcca cggagggcgt gcgcgcgccg tcgaggaggg aagcctccac atcgacgtcg
      961 ccttcctcgc ggcgccagcc tgcgacaggc tcggcaactt caccggttcg gcgggaaaga
     1021 cggcctgcgg ctcgatgggg tacgcgatgc tcgacgcgcg ctacgcggat catgtgatcg
     1081 ccgtgaccga caaccttgcg gagtacccgc tcgcgccgcg catctcggtt ccacagtacc
     1141 tggtggatca ggtggtcgtg gtcgactcca tcggcgatcc ctcaaagatc gccaccgggg
     1201 ccacccgcgt aacccgcgac cccgtgcagc tcaggatcgc ggagctggcg ttcgggctcc
     1261 tcaaggcatc cgggatg
//
LOCUS       DAOTIL010000910         1273 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminobacteriaceae bacterium isolate AMR_MDS_3026
            NODE_128011_length_1273_cov_3.380131, whole genome shotgun
            sequence.
ACCESSION   DAOTIL010000910 DAOTIL010000000
VERSION     DAOTIL010000910.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868050
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminobacteriaceae bacterium (wastewater metagenome)
  ORGANISM  Aminobacteriaceae bacterium
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae.
REFERENCE   1  (bases 1 to 1273)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1273)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:19:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,224
            CDSs (total)                      :: 3,198
            Genes (coding)                    :: 3,184
            CDSs (with protein)               :: 3,184
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1273
                     /organism="Aminobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_128011_length_1273_cov_3.380131"
                     /isolate="AMR_MDS_3026"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:3034609"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(261..941)
                     /gene="rlmB"
                     /locus_tag="P5201_14425"
     CDS             complement(261..941)
                     /gene="rlmB"
                     /locus_tag="P5201_14425"
                     /inference="COORDINATES: protein motif:HMM:TIGR00186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (guanosine(2251)-2'-O)-methyltransferase RlmB"
                     /protein_id="HRV99791.1"
                     /translation="MQKVYLGKNVSDRFSLQIGDLAEKGKVTVLRVTPEALTDMCGTN
                     NHQGVACRSGDVSMLELQDILPLLDGKKGPVLLVLLDHVQDPHNLGAVVRTAEACGAM
                     AVIFPKRRAALPGGTVVKVSAGAALRVPMVAVTNVAQTVREIQQNGFWTIGLDNKNGR
                     SLWSETMPEKTLLVVGAEGEGLSRLVAETCDETVRIPVAGATGSLNASVACAVGMFEW
                     ARKWGAQS"
BASE COUNT          260 a          372 c          290 g          351 t
ORIGIN      
        1 aagatcctcc agtttgatgc acgaagattc ctgttgttgc catcgctact ccaacggatt
       61 taagaagtga ggtgcggcgc ctgtcgacgg gacttccctt tttttctttc tgagagctgg
      121 agtaaaagaa gaatctcaaa tcatcaatta aggatccata gattaaaagc aaattagctt
      181 ttgtttcaac gcgccgattc agggagtaat ccttgaacct gcgcgttgtt tgttgaagct
      241 gaaaactttt tacagcaaaa ctaggattgc gccccccact tcctcgccca ttcaaacatt
      301 ccgacggcgc aagcgacgct tgcgttcagt gaacctgttg caccggcaac cggtatccgg
      361 acggtctcat cgcacgtttc cgccacaaga cgggagagtc cctccccctc cgcgcctact
      421 acaagcaggg tcttttccgg catggtctcc gaccagagcg agcggccgtt cttattatcc
      481 agccctatag tccagaatcc gttctgttgg atctctctga ctgtttgtgc aacattcgtt
      541 actgccacca tgggaacgcg taatgccgct ccggcgctta ctttgacgac agtccctcct
      601 ggaagggcgg cgcgtctttt agggaagatg actgccattg ccccgcaagc ctcggcagta
      661 cgcacaacag ccccgaggtt gtggggatcc tggacgtgat caaggagcac aagaagtaca
      721 ggccctttct tcccgtcaag aagaggaaga atatcctgca gctcaagcat cgatacatct
      781 ccgcttctgc aggccacccc ctgatgatta ttggttccgc acatatcggt gagcgcttca
      841 ggcgttactc tcaagacggt gactttccct ttttctgcca aatcgcctat ctggagcgaa
      901 aaacggtcgc tcacattttt accgagatag accttctgca ctttgtcagg cgattccttc
      961 aaaagctgga ggacgacctg ccttccccat tgaatatcct cacttcgctt ctcttcgctt
     1021 cgttctcctc tcggtggaac tgatttgcga taattccgtt tctcctcgtt tttcggcgcg
     1081 cttcgaactc ttttgtccgg agttcggtcc attctttttt ccactctttt tcaccctttc
     1141 ggcgatttgc ttcgcatatt cttgcagata gtatgcggta tgtcccttct ctcccgtcat
     1201 gatatcttca ggggttccgg ttgcgacgag acggcctcct ccgctccccc cttccggtcc
     1261 caggtcaacg atg
//
LOCUS       DAOTIL010000911         1273 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminobacteriaceae bacterium isolate AMR_MDS_3026
            NODE_128107_length_1273_cov_1.779146, whole genome shotgun
            sequence.
ACCESSION   DAOTIL010000911 DAOTIL010000000
VERSION     DAOTIL010000911.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868050
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminobacteriaceae bacterium (wastewater metagenome)
  ORGANISM  Aminobacteriaceae bacterium
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae.
REFERENCE   1  (bases 1 to 1273)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1273)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:19:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,224
            CDSs (total)                      :: 3,198
            Genes (coding)                    :: 3,184
            CDSs (with protein)               :: 3,184
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1273
                     /organism="Aminobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_128107_length_1273_cov_1.779146"
                     /isolate="AMR_MDS_3026"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:3034609"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..320)
                     /locus_tag="P5201_14430"
     CDS             complement(<1..320)
                     /locus_tag="P5201_14430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013049263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HRV99792.1"
                     /translation="MALKQSSSAHSKGAFLPDASSVLYALLAGGVLIFILWPTLAILK
                     ESVLVDGVFSLERYRDLLTGNRTLLKNSLFVGWWTTVLAVLIGLCCALYVTRTSFRGK
                     RAVLG"
     gene            complement(298..>1273)
                     /locus_tag="P5201_14435"
     CDS             complement(298..>1273)
                     /locus_tag="P5201_14435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013049262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HRV99793.1"
                     /translation="VGTAAFAEADKPLAGRSISIFTAATGKDETFAEFTKDTGIEVHY
                     LEISSGEVLSRLRASGGKNLADAWFGGGVDSYIAAGEEGFLEPYRSPEAELIPDEYKS
                     PEGYWTGLSLVAVDLIVNTDVLKEKNLSLPKTWLDLGKPEYKDELMMSNPTISGTNYA
                     VLFHLIQLYGEEAGWDLLRKMDANIPFYTKRGAAPPNKAAMGEVAIGIDPYDVGVQIV
                     AQGYPVVSVFPEEGVPGAISPVALMKGAKDMEAAKAFVDWCLSKRGQEVQMAYTAKVG
                     TRPDAEVPEYLQGLKNAKIVVADPVKVGEMRTSILDRWQQEFGTKAEQ"
BASE COUNT          221 a          429 c          384 g          239 t
ORIGIN      
        1 cccagcacag cccgcttccc ccggaaggag gtgcgcgtca cgtagagggc gcagcagagg
       61 ccgatcagca ccgcaaggac ggtcgtccac catccgacga agaggctgtt cttcagcaac
      121 gtccggttgc ccgtcagcag gtcgcggtag cgttccaggc tgaatacgcc gtcgaccagg
      181 acgctctcct tcagaatggc gagcgtcggc cagaggatga aaataagaac gccccccgcc
      241 aggagggcgt acagaacgga ggaggcgtcc gggaggaacg cccccttcga atgtgcgcta
      301 ctgctctgct ttagtgccaa attcctgctg ccacctgtcg agaatactcg tgcgcatctc
      361 gccgaccttc accggatccg ccacgacgat cttcgcgttc ttcagcccct gcagatactc
      421 ggggacctcg gcgtcgggac gcgttcctac cttcgccgtg tacgccatct gcacctcctg
      481 cccgcgcttc gagaggcacc agtcgacgaa ggccttcgca gcctccatat ccttggcccc
      541 tttcatcagg gcgacgggcg agatggcgcc cgggaccccc tcttcgggga agacggagac
      601 gacggggtac ccctgcgcga cgatctgcac gccgacgtcg tacggatcga tcccgatggc
      661 cacttcgccc atcgcggcct tgttgggggg cgccgcgccg cgcttcgtgt agaaggggat
      721 gttcgcgtcc atcttccgga gaagatccca tcccgcctcc tcgccgtaaa gctggatcag
      781 gtggaaaaga acggcgtagt tcgtcccgga gatggtcggg ttgctcatca tgagctcgtc
      841 cttgtactcc ggcttgccca ggtcaagcca ggtttttggt agcgacaggt tcttctcctt
      901 tagcacgtcg gtgttcacga tcaggtcgac ggccacgagc gaaagtccgg tccagtaccc
      961 ttcgggagac ttgtactcat ccggaatcag ttcggcctcg ggagagcggt acggctcgag
     1021 gaaaccttcc tcgccggccg cgatgtagct gtcgacgccg ccgccgaacc acgcgtcggc
     1081 caggttcttc ccgccggaag cccgcagtct cgaaagcacc tcgcccgacg atatttcgag
     1141 gtaatgcacc tcgatgccgg tgtccttcgt gaattcggcg aacgtctcgt ccttgcccgt
     1201 cgcggcggtg aagatgctga tgctccgccc cgccaggggc ttgtccgcct cggcgaacgc
     1261 cgccgtcccg acc
//
LOCUS       DAOTIL010000912         1272 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminobacteriaceae bacterium isolate AMR_MDS_3026
            NODE_128150_length_1272_cov_3.857025, whole genome shotgun
            sequence.
ACCESSION   DAOTIL010000912 DAOTIL010000000
VERSION     DAOTIL010000912.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868050
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminobacteriaceae bacterium (wastewater metagenome)
  ORGANISM  Aminobacteriaceae bacterium
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae.
REFERENCE   1  (bases 1 to 1272)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1272)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:19:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,224
            CDSs (total)                      :: 3,198
            Genes (coding)                    :: 3,184
            CDSs (with protein)               :: 3,184
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1272
                     /organism="Aminobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_128150_length_1272_cov_3.857025"
                     /isolate="AMR_MDS_3026"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:3034609"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            360..>1272
                     /locus_tag="P5201_14440"
     CDS             360..>1272
                     /locus_tag="P5201_14440"
                     /inference="COORDINATES: protein motif:HMM:NF036965.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ASKHA domain-containing protein"
                     /protein_id="HRV99794.1"
                     /translation="MTYRCNKKNCTRCGACSGLGVLSKPLRGLSAPLGSGEETSSRVF
                     SAAIDLGTTTLACYLADVQDGRILAAASARNPQASWGPDVISRIVRASESPGALHELA
                     DVVRNHIGALVANLAGDAGLDVRNCREIAIAGNSVMELLFLGISPEPLGSAPFALPAR
                     SFAPRSVGELEIPFLPPETPVHIFPLIDGFVGGDTTALLYEITEAAALSGSQALTRLV
                     IDFGTNGEIALIHKGRIVACSTAAGPAFEGASVRQGMPALPGAVCGIVSDGGATYCRT
                     VNNAPAIGLCGSGLVDAVALLLREGVLN"
BASE COUNT          245 a          410 c          399 g          218 t
ORIGIN      
        1 gtccgggagc aagaatacgg gaaccctcgg acggagctgc caccgtgagc gtgacggcct
       61 ccgcatcggg aagacccagc agcagcgagg cgcacaggga gaagagcaac atgaagcgtc
      121 gcatgaaaac aacacaccct ttccaagatg atgagagaga tcaaagaaaa tcataccacc
      181 aagcggccgc ttcgtcctcg tctttcacac cctgatcaac gggaacttcc gtccgcgcct
      241 cttttccggt atcatggcgg agcgccgccg acgcagggcg cgagcgccgg gagccccggc
      301 cgtaaacgaa gcggacgcgc cgctcttcat tatttgatgc agggaaagga aaaaagacaa
      361 tgacctacag atgcaataaa aaaaactgca cccgctgcgg cgcatgttcc ggactcggag
      421 tgctctccaa gcccctccgc ggactttcgg cgccgcttgg ttccggtgaa gagacatcgt
      481 cccgagtgtt ttcggcggcg atcgacctcg ggacgaccac gctcgcctgc tatcttgcgg
      541 atgttcagga tggtcgcatt ctcgccgcgg cgtcggcgcg gaatcctcag gcttcttggg
      601 gaccggacgt catctccaga atcgtccgcg cctcggagag ccccggtgcg ttgcacgagc
      661 ttgcggacgt cgtgaggaat cacatcggcg cgctcgtggc gaatctcgcc ggagacgccg
      721 ggctcgatgt ccggaactgc cgcgaaattg ccattgccgg caacagcgtc atggagctgc
      781 tcttcctggg catatccccg gaaccgctgg gaagcgcacc gttcgcgctc ccggcgcgaa
      841 gcttcgctcc ccggagcgta ggcgaactcg aaattccgtt cctccctccg gagacgccgg
      901 tccacatctt ccccctgatc gatggatttg tcggcggcga cacgaccgcg ctgctctacg
      961 agatcacgga agcagccgcc ctctcgggga gtcaggcgct gacacggctc gtcatcgact
     1021 tcgggacgaa tggagagatc gcgctcatcc acaaggggag aatcgtcgcc tgctccaccg
     1081 ccgcagggcc cgcgttcgaa ggcgcgagcg tccgccaggg aatgcccgcg ctgccgggcg
     1141 ctgtttgtgg cattgtctcc gacggggggg cgacgtactg tcgcacggtg aataacgcgc
     1201 ctgccatcgg actctgcggc agcgggctcg tcgacgccgt cgcgctgctt ctgcgggagg
     1261 gtgtgctcaa cg
//
LOCUS       DAOTIL010000913         1272 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminobacteriaceae bacterium isolate AMR_MDS_3026
            NODE_128193_length_1272_cov_2.623665, whole genome shotgun
            sequence.
ACCESSION   DAOTIL010000913 DAOTIL010000000
VERSION     DAOTIL010000913.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868050
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminobacteriaceae bacterium (wastewater metagenome)
  ORGANISM  Aminobacteriaceae bacterium
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae.
REFERENCE   1  (bases 1 to 1272)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1272)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:19:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,224
            CDSs (total)                      :: 3,198
            Genes (coding)                    :: 3,184
            CDSs (with protein)               :: 3,184
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1272
                     /organism="Aminobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_128193_length_1272_cov_2.623665"
                     /isolate="AMR_MDS_3026"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:3034609"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..332
                     /locus_tag="P5201_14445"
     CDS             <1..332
                     /locus_tag="P5201_14445"
                     /EC_number="2.7.1.6"
                     /inference="COORDINATES: protein motif:HMM:NF020133.2"
                     /note="catalyzes the formation of alpha-D-galactose
                     1-phosphate from D-galactose in galactose metabolism;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="galactokinase"
                     /protein_id="HRV99795.1"
                     /translation="ARVLSAVAALESGKIDEFGRLLNESHVSLQFDYETTGRELDCLA
                     ELSWQSGECLGARMTGAGFGGSALAIVRSDGVDAFTRFVGERYRRYIGYDCSFFRSVA
                     GDGARRI"
     gene            complement(378..1226)
                     /locus_tag="P5201_14450"
     CDS             complement(378..1226)
                     /locus_tag="P5201_14450"
                     /EC_number="3.5.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005660713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016812 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds, in cyclic amides
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclase family protein"
                     /protein_id="HRV99796.1"
                     /translation="MFACLSKKRARIVLFLGVLLTGFFLFTGSAAAEPTLLEIYDTLK
                     TKEFVDLTHTFDTEIPHWPGFPKEKRETIYWYDKGVGTMGYGFFAQVFCHVGQWGTHV
                     DAPAHFVRGTRTLDQIDVKEMLLPLVVIDVHEKAAANPDYALTLDDVKEWEKKYGPVP
                     EGAFVAMRTDWSKKWPDAAAMRNADQAGTAHYPGWGLEAMKYLFEERKITASGHEPTD
                     TDPGVSTSKGDYTCESYVLGQDKYQIELLANLDKVPEHGAIVVVAFPKPFFGSGFPAR
                     VFAILP"
BASE COUNT          233 a          368 c          389 g          282 t
ORIGIN      
        1 acgcgagggt gctttcggcc gtcgccgcct tggaatcggg aaaaatcgac gagttcggcc
       61 gtctgctcaa cgaatcgcac gtgtcattgc agttcgacta cgaaaccacg ggaagagagc
      121 tcgactgcct tgccgaactt tcgtggcaga gcggcgagtg cctcggcgca cggatgaccg
      181 gcgccgggtt cggaggaagc gcgcttgcca ttgtccgaag cgacggagtg gatgcgttca
      241 ctcgttttgt gggagagcgg taccggcggt atatcggcta cgactgttcc ttcttcagga
      301 gcgtcgccgg agatggagcg cgcagaatat agttcgggcg ccctctattc caggggcgcc
      361 cgcgttgttt cctgagctta ggggagtata gcgaagactc tcgcggggaa tcccgagccg
      421 aagaacggct tggggaaggc gaccacgacg atcgcgccgt gctcgggaac cttgtcaaga
      481 tttgccagaa gttctatctg gtacttgtcc tgtcccagta cgtacgactc gcaggtgtag
      541 tcgcccttcg aggtcgacac acccggatcc gtgtccgtgg gttcatgtcc ggacgcagtg
      601 atcttccgtt cctcgaagag gtacttcatg gcttcaagac cccatcccgg gtagtgcgcc
      661 gttcccgctt ggtccgcgtt tcgcatcgcc gcagcgtccg gccatttctt cgaccagtcg
      721 gtgcgcatcg cgacgaatgc tccttcagga acggggccgt acttcttctc ccactccttt
      781 acgtcgtcaa gcgtgagcgc gtagtccgga tttgccgccg ctttttcgtg gacgtcgatc
      841 accacgagag gaagcagcat ctcctttacg tcgatctggt cgagggtcct ggtcccccgg
      901 acgaagtggg cgggagcgtc gacgtgcgtt ccccactgcc cgacatggca gaagacctgg
      961 gcgaagaagc cgtagcccat cgtgccgacg cctttatcgt accagtagat cgtctcccgt
     1021 ttctccttcg ggaatcccgg ccagtgcgga atctcggtgt cgaaggtgtg cgtcaggtcg
     1081 acgaactcct tcgtcttgag ggtatcgtag atttcgagga gcgtgggctc ggcggccgcc
     1141 gaacctgtga agagaaagaa gcctgtaagc agtacgccga gaaagagaac gatacgcgct
     1201 cttttttttg acaagcaagc gaacattttt tgacctccgt ttccagtcta tccggtaccc
     1261 ctgaacgctt cg
//
LOCUS       DAOTIL010000914         1270 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminobacteriaceae bacterium isolate AMR_MDS_3026
            NODE_128470_length_1270_cov_3.395885, whole genome shotgun
            sequence.
ACCESSION   DAOTIL010000914 DAOTIL010000000
VERSION     DAOTIL010000914.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868050
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminobacteriaceae bacterium (wastewater metagenome)
  ORGANISM  Aminobacteriaceae bacterium
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae.
REFERENCE   1  (bases 1 to 1270)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1270)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:19:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,224
            CDSs (total)                      :: 3,198
            Genes (coding)                    :: 3,184
            CDSs (with protein)               :: 3,184
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1270
                     /organism="Aminobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_128470_length_1270_cov_3.395885"
                     /isolate="AMR_MDS_3026"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:3034609"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..705
                     /locus_tag="P5201_14455"
     CDS             <1..705
                     /locus_tag="P5201_14455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014163687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HRV99797.1"
                     /translation="MGPSGSGKSTLIRCLIRLVEPTSGEIIVNGENVTMMHKRELVDF
                     RREKIAMVFQHYGLLPHRTVLDNVCFGLKLRGEEKEARRAKGVEALEQVGLKGWEKYY
                     PASLSGGMRQRVGIARALVMDASILLMDEPFSGLDPLIRRELQDEMIRLQKEMHKTIF
                     FVTHDLSEALRMGDRMAIMKKGEIVQVGSPDEVIASPADEYVAKFVQDERDQVQRAEQ
                     VLAREHTRKEAEAHVS"
     gene            695..>1270
                     /locus_tag="P5201_14460"
     CDS             695..>1270
                     /locus_tag="P5201_14460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009200302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HRV99798.1"
                     /translation="MFPESFQVHVAPAVNRFINDLIAAYAPVFDSISAGILSFLLTVS
                     EILVFIPWWGYILAVGLLSWYSSKKFVASIVLAALPFLIGMFGLWAMALESLSIIITA
                     VLMALIIGLPVGVLMAEVRIIATILRPILDGMQTMPSFVYLIPAMMLFGLGRVPAVLA
                     TLIYALPPVIRLTALGLNQVPKPVEEAALAYG"
BASE COUNT          250 a          376 c          367 g          277 t
ORIGIN      
        1 atggggccct cgggcagcgg caagtctacc cttatccgct gccttatccg ccttgtcgag
       61 ccgacttccg gagaaatcat cgtcaatggc gaaaacgtca cgatgatgca caagagagaa
      121 ctggtggatt tccgccgcga aaagatcgcc atggtcttcc agcactacgg acttctgcct
      181 caccgcaccg tgctcgacaa cgtctgcttc ggactcaaac tccggggaga ggagaaggaa
      241 gcccggcgcg caaagggcgt agaggccctt gaacaggtcg gcctcaaggg gtgggagaag
      301 tactatcccg catccctgag cggcggtatg cgacagaggg tcggtatcgc ccgcgcgctc
      361 gtgatggacg cttcgatact tctcatggac gagccgttca gcggactgga ccctcttatc
      421 cggcgcgaac tgcaggacga gatgatccgt ctgcagaagg agatgcacaa gaccatcttc
      481 ttcgtgacgc acgacctctc cgaggccctt cgaatgggag accgaatggc cattatgaaa
      541 aaaggggaga tagtacaggt aggctctccc gacgaagtca tcgcaagccc tgccgacgag
      601 tacgtggcga agttcgttca ggacgaacgc gaccaagtgc agcgggccga acaggttctg
      661 gcgagagagc atacgagaaa ggaggcggag gcccatgttt cctgagtctt ttcaagtaca
      721 cgtggcgccg gccgtcaaca ggttcatcaa cgacctgata gcggcctacg cacctgtttt
      781 cgactctatc tccgcaggga ttctctcctt tcttctcacc gtcagcgaga tactcgtttt
      841 tattccctgg tgggggtata tcctcgcggt aggcttgctt tcatggtatt cgtcgaagaa
      901 attcgtcgcc tccatcgttt tggcggcgct cccctttctc atcgggatgt tcggcctgtg
      961 ggccatggct cttgagagcc tctccatcat catcaccgcc gtgctgatgg cgttgatcat
     1021 cggtcttccg gtcggcgttc tcatggcgga ggtgcgcatc atcgcgacga ttctccggcc
     1081 gattctcgac ggaatgcaga ccatgccgag ctttgtctac ctcattccgg cgatgatgct
     1141 cttcggtctc gggagggttc ccgcagtcct cgcaacgctt atctacgcgc tgcccccggt
     1201 gatccgcctt actgctcttg gtctcaacca ggtgcccaaa ccggtcgagg aagccgcgct
     1261 ggcctacggg
//
LOCUS       DAOTIL010000915         1269 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminobacteriaceae bacterium isolate AMR_MDS_3026
            NODE_128627_length_1269_cov_3.343493, whole genome shotgun
            sequence.
ACCESSION   DAOTIL010000915 DAOTIL010000000
VERSION     DAOTIL010000915.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868050
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminobacteriaceae bacterium (wastewater metagenome)
  ORGANISM  Aminobacteriaceae bacterium
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae.
REFERENCE   1  (bases 1 to 1269)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1269)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:19:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,224
            CDSs (total)                      :: 3,198
            Genes (coding)                    :: 3,184
            CDSs (with protein)               :: 3,184
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1269
                     /organism="Aminobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_128627_length_1269_cov_3.343493"
                     /isolate="AMR_MDS_3026"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:3034609"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..385)
                     /locus_tag="P5201_14465"
     CDS             complement(<1..385)
                     /locus_tag="P5201_14465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013048864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-layer homology domain-containing protein"
                     /protein_id="HRV99799.1"
                     /translation="MKKILALVAVVALVAFAAPAFANVNPFMDVPMNHWAYDAIGQLA
                     ARGVLSGYPDGTYKGNQPMTRYEAASAIARALAVVDMTKASKQDVEMLKRLVVEFKDE
                     LDALGVKVDKIDSRLAVIEERLGGWK"
     gene            complement(733..>1269)
                     /locus_tag="P5201_14470"
     CDS             complement(733..>1269)
                     /locus_tag="P5201_14470"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013257.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 9 protein"
                     /protein_id="HRV99800.1"
                     /translation="MRKRAASLLGEHASRPFILGAIGASKEIKRWPVGRWKEFTAYAV
                     RAGLQVVLAGEGEEEKTAASVIETAVCSPLVRNVVGKIPLADLGGAISLSSAVVAGDT
                     GILHIARMLDVPSFALLGPTPLPEGLGLASPERTFWTRCPLKACNNWRCTQECLGTIS
                     SSDVWNVVSAFLERRSSK"
BASE COUNT          261 a          377 c          349 g          282 t
ORIGIN      
        1 gcttccatcc gccgaggcgc tcctcgatta cggcgagtct gctgtcgatc ttgtcgacct
       61 tgacgccaag cgcatcaagc tcgtccttga actcgacaac aagtctcttc agcatttcga
      121 catcctgctt gctggccttg gtcatgtcga ccacggccaa ggcacgggcg atagcggaag
      181 cggcttcgta acgggtcatg ggctggttgc ccttgtaggt gccatcggga tagccggaaa
      241 gaactccccg agcggcaagc tggccgatgg catcatatgc ccagtggttc atgggaacat
      301 ccatgaacgg gttgacgttg gcgaacgcag gagcggcgaa cgcaacaagc gcgactactg
      361 caacaagtgc aagaatcttt ttcattgtta tgtaaccccc ttggtttagt gtgttttgta
      421 tagcgcgggt attccttgct ccgcttcggg ggtccgaatg aagtttcctc gtttcctttt
      481 tcttctcccc tatgccgtgg tccggaatgt atccgccttc atctttcgct cgtccttgtt
      541 ggcagggggt cgtcgcacct cctttcacgc cgtcgggatt tccacacgaa agaacagaag
      601 ctgattcatc caaggtcaaa ttgtcaaagt gcttcaattc gactgctgag agcgattgta
      661 gccgaacgca ccttctttga gaataggact taagactcat cttgcggaaa gtacgtaaaa
      721 aagtctcgct tttcactttg aagaccgcct ctccagaaaa gcggaaacga cattccacac
      781 gtccgaagag gagatcgttc ccaggcactc ctgcgtgcat cgccagttgt tgcatgcttt
      841 cagagggcac cgcgtccaga acgtccgttc ggggcttgca aggcccagtc cttcgggaag
      901 cggcgtcggc cccagaagcg cgaaggaggg aacatccagc attcgggcga tgtgaaggat
      961 accggtatct ccggctacaa cggcgctgga gagcgagatc gctcccccaa ggtccgcgag
     1021 cggaattttc ccgaccacgt tgcggaccag aggagaacac acggcggttt cgatcacgga
     1081 cgcggcggtt ttctcctcct cgccttcccc cgccaaaacg acctgaaggc cggcgcgcac
     1141 cgcatacgcg gtgaattcct tccaacgtcc cacgggccat cgcttgatct ccttgcttgc
     1201 gccgatagcc ccgagtatga acggacggga ggcatgctcc cccagcaggg aggccgcccg
     1261 cttccgcat
//
LOCUS       DAOTIL010000916         1267 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminobacteriaceae bacterium isolate AMR_MDS_3026
            NODE_128972_length_1267_cov_2.661716, whole genome shotgun
            sequence.
ACCESSION   DAOTIL010000916 DAOTIL010000000
VERSION     DAOTIL010000916.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868050
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminobacteriaceae bacterium (wastewater metagenome)
  ORGANISM  Aminobacteriaceae bacterium
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae.
REFERENCE   1  (bases 1 to 1267)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1267)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:19:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,224
            CDSs (total)                      :: 3,198
            Genes (coding)                    :: 3,184
            CDSs (with protein)               :: 3,184
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1267
                     /organism="Aminobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_128972_length_1267_cov_2.661716"
                     /isolate="AMR_MDS_3026"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:3034609"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..301
                     /locus_tag="P5201_14475"
     CDS             <1..301
                     /locus_tag="P5201_14475"
                     /EC_number="4.2.2.n1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014806165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="lytic transglycosylase domain-containing
                     protein"
                     /protein_id="HRV99801.1"
                     /translation="KTDAVSYKGAMGLMQLMPGTAKMLGVDDPFDPVQNLEGGIKYLS
                     QLADKYNGDLTKTLAAYNAGPGRVDAYGGIPPFAETQKYVRSILALYERNSEGDS"
     gene            301..963
                     /locus_tag="P5201_14480"
     CDS             301..963
                     /locus_tag="P5201_14480"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="MgtE intracellular region"
                     /protein_id="HRV99802.1"
                     /translation="MPEEEMELERAEGEVVPPPKKKKKKTKGSKLPFFLLVLLMAAGV
                     GAGLHIAGAWDARPLLYASVPKIPWIGNDLAKLTGIPEEYTLTVEQRRRLELQRWNDR
                     LDELERSLKERETALEALSADLEARSISVEKKEAEFTLKENKPPESPEEEKAYVDMLM
                     RTYQEISPRRAALIIEQLREDLAVKMLAAMPQDARASILGRMEAARAARLTERLAAGG
                     SR"
     gene            985..>1267
                     /locus_tag="P5201_14485"
     CDS             985..>1267
                     /locus_tag="P5201_14485"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRV99803.1"
                     /translation="MFSALFPVHKDGMTDVLPGAETPGSVKNGGDGEKKEQFAKLLFV
                     SLETADDGKNTTTETPGETPLWTNGARSLTALAASLLGENAGQEAAVIAD"
BASE COUNT          316 a          322 c          408 g          221 t
ORIGIN      
        1 aaagaccgac gccgtctcgt acaagggcgc catggggttg atgcagctta tgccgggaac
       61 ggcgaagatg ctcggggtgg atgatccgtt cgatccggtg cagaatcttg aagggggtat
      121 aaaatacctc tcgcaactgg ccgataagta taacggggac cttacaaaaa ctcttgccgc
      181 gtacaacgcg ggacccggaa gggttgacgc ctacggggga attcccccct tcgccgagac
      241 gcagaagtat gtacggagca ttctcgctct ctacgaacgc aattctgaag gagatagctg
      301 atgcccgaag aagagatgga gctcgagaga gcggagggcg aggttgtccc tccaccaaag
      361 aagaaaaaaa agaaaacaaa aggaagtaag ctgccgtttt tcctcctggt gctcttaatg
      421 gcggccggag tcggcgccgg gttgcatatc gccggagcgt gggatgcacg gcctcttttg
      481 tacgcgagcg ttccgaagat cccgtggata ggaaacgatc tcgccaagct gaccggcatt
      541 ccggaagaat acacgcttac cgtcgagcag cggcgcaggc ttgaacttca gcgatggaac
      601 gaccggctcg acgaactgga gcgcagcctc aaagaacggg agacagccct cgaagcgctt
      661 tccgccgacc tcgaagcacg aagtatttcg gtcgagaaga aggaggcgga gttcacttta
      721 aaggagaaca agcctccgga gagtcctgaa gaggaaaaag cgtacgtcga catgctgatg
      781 cggacgtacc aggaaatatc accgaggcgg gcggcgctga tcatcgagca gttgcgcgag
      841 gatctggcgg tgaagatgct cgccgcgatg ccacaggacg cgagagcgtc cattttgggc
      901 cgcatggagg cggccagggc ggcaaggctg acggagcggc tggcggccgg agggtcgcga
      961 taacgattcg ggaggtgcgg cttcatgttt tcagccttgt ttccagtgca taaagatgga
     1021 atgaccgacg tccttccagg cgccgagacg ccgggaagcg taaagaacgg aggcgacggc
     1081 gaaaaaaagg agcagttcgc caaactgctc tttgtttcgt tggagactgc ggacgacggt
     1141 aaaaatacga ccacggaaac ccccggggaa acccctttgt ggacaaacgg cgcccgttcg
     1201 ttgaccgcac ttgccgcctc tcttttagga gagaatgcag gccaggaagc agccgttatt
     1261 gcggatg
//
LOCUS       DAOTIL010000917         1266 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminobacteriaceae bacterium isolate AMR_MDS_3026
            NODE_129056_length_1266_cov_4.446738, whole genome shotgun
            sequence.
ACCESSION   DAOTIL010000917 DAOTIL010000000
VERSION     DAOTIL010000917.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868050
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminobacteriaceae bacterium (wastewater metagenome)
  ORGANISM  Aminobacteriaceae bacterium
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae.
REFERENCE   1  (bases 1 to 1266)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1266)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:19:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,224
            CDSs (total)                      :: 3,198
            Genes (coding)                    :: 3,184
            CDSs (with protein)               :: 3,184
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1266
                     /organism="Aminobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_129056_length_1266_cov_4.446738"
                     /isolate="AMR_MDS_3026"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:3034609"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..213
                     /locus_tag="P5201_14490"
     CDS             <1..213
                     /locus_tag="P5201_14490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013048568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54 modulation/S30EA ribosomal C-terminal
                     domain-containing protein"
                     /protein_id="HRV99804.1"
                     /translation="VAEPELPIPPEEEIVKVKRFPLRPMSAKEATMQMDLLGHEFFLF
                     KNAESGIVNVVYKRKDGGYGLLEPSE"
     gene            402..743
                     /gene="hypA"
                     /locus_tag="P5201_14495"
     CDS             402..743
                     /gene="hypA"
                     /locus_tag="P5201_14495"
                     /inference="COORDINATES: protein motif:HMM:TIGR00100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008901 - ferredoxin hydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase maturation nickel metallochaperone
                     HypA"
                     /protein_id="HRV99805.1"
                     /translation="MHELSLADAIVSALLKMCEDEAWGRVKKVRLRVGALRQVFPEML
                     EFAFSTVVKDTALSGALLEIEEIPLEWRCLECGNRWREDSGVCPLCGSFNRETLAGME
                     LEIHSVEVEET"
     gene            740..>1266
                     /gene="hypB"
                     /locus_tag="P5201_14500"
     CDS             740..>1266
                     /gene="hypB"
                     /locus_tag="P5201_14500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013048566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016530 - metallochaperone activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase nickel incorporation protein HypB"
                     /protein_id="HRV99806.1"
                     /translation="MNKIVTLQQSVMAEDEKFAAAIRDRNTRMGLLMVNIIGSPGCGK
                     TTLLEKTALHAGLSMAVIEGDLATDRDAERLARLGVPVVQINTKGGCHLEANVVEKAM
                     ESLPLDTLDVLFIENVGNLVCPAEFDLGEDFKVAVLSAPEGADKPLKYPYLFTSAKAV
                     LLTKTDLQHRFRGSE"
BASE COUNT          289 a          312 c          376 g          289 t
ORIGIN      
        1 gtcgcggagc ctgagcttcc tatccctccc gaagaggaaa tcgtgaaggt gaaacgtttc
       61 cccctgcgtc ctatgagcgc aaaagaagcg acgatgcaga tggatcttct tgggcatgag
      121 tttttcttgt tcaagaacgc cgagtccggg atagtgaacg tggtgtacaa gcggaaggac
      181 ggcggatacg gccttcttga gccttcggag tgattttcag tcgcagcggg cgcccctcgc
      241 gcgggggctt cggatcgcgg taactcgatc tgtaccggtg aatgtgtgta ttctccggtt
      301 tttcccccgc ctgaacgggt atggcaggtt tttccccgtc agtaaccgtc gatgcgaaaa
      361 ggacccgaaa gggtcctttt tttctggaaa agaggaacgg catgcatgaa ctctctcttg
      421 ccgatgcgat tgtatcggcc cttttaaaaa tgtgcgagga cgaagcatgg ggcagggtaa
      481 aaaaagttcg ccttcgggtc ggcgcccttc gccaggtttt tcccgaaatg ctcgaattcg
      541 cattcagtac agtggttaaa gatacggcgc tttccggggc gctcctcgaa atcgaggaga
      601 ttcctctgga atggagatgc cttgaatgcg ggaatcgctg gcgggaggat tccggagtct
      661 gtccgttatg cggctctttc aacagggaga cgcttgcagg gatggaactg gaaattcatt
      721 ctgtggaggt cgaggaaaca tgaataagat agttacgctg caacagtccg tgatggcgga
      781 ggatgagaaa ttcgccgccg cgatcaggga cagaaatacc cgaatgggcc ttctgatggt
      841 caatatcatc ggttctcccg gttgcgggaa gacaaccctg ctggaaaaaa cagcgcttca
      901 tgcaggactc tcgatggctg taatcgaagg agatcttgcc acagaccgcg atgcggaacg
      961 attggctcgc cttggcgtac ccgtcgtgca gatcaacaca aaaggcggat gccaccttga
     1021 agccaacgtt gttgaaaaag cgatggaatc gcttccgctg gatacgctgg acgttctttt
     1081 catcgagaac gtcggcaacc ttgtctgccc ggcggaattc gacctcggtg aggatttcaa
     1141 ggttgcggtg ctgagcgctc cggaaggtgc cgataaaccg ctgaaatacc cgtatctctt
     1201 cacttccgcg aaagcggtgc tgctgaccaa aaccgatctg cagcaccgct ttcgcggaag
     1261 tgaaga
//
LOCUS       DAOTIL010000918         1262 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminobacteriaceae bacterium isolate AMR_MDS_3026
            NODE_129679_length_1262_cov_3.560066, whole genome shotgun
            sequence.
ACCESSION   DAOTIL010000918 DAOTIL010000000
VERSION     DAOTIL010000918.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868050
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminobacteriaceae bacterium (wastewater metagenome)
  ORGANISM  Aminobacteriaceae bacterium
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae.
REFERENCE   1  (bases 1 to 1262)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1262)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:19:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,224
            CDSs (total)                      :: 3,198
            Genes (coding)                    :: 3,184
            CDSs (with protein)               :: 3,184
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1262
                     /organism="Aminobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_129679_length_1262_cov_3.560066"
                     /isolate="AMR_MDS_3026"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:3034609"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1116)
                     /locus_tag="P5201_14505"
     CDS             complement(<1..1116)
                     /locus_tag="P5201_14505"
                     /inference="COORDINATES: protein motif:HMM:NF012602.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HRV99807.1"
                     /translation="MTDMRRKTTACPYDCPDACAMKGMFDGASVTLEPNRDLPYTTFL
                     CGKGLRWASRATSSERLTTPMLRREGALVPVSWDEAVDVWAEKVRSAVSRHGPLSLMH
                     LSSAGSLYFSKLLLPHLFAALGGYTTKKGNLCSSAGSFGLAESFGEVPVTRPESLSDH
                     ARGVLFWGRNALETHAHVVPLLQRVRGRGGEIASLEIRETPTTRFADRWWRVNPGGDW
                     ALAAWLCARLLEEGASDGWRERVENPEEFERTARSLDGAFLLRTAGVSAECASELLEW
                     LMRFTPVTHYAAFGAQRYLHGDAQFRWIGALAVLLGAFSGPGAGLAFSKDEMALFPFA
                     LLPKVAYVRRLPVATWPLELESLDPPVEALSISGANPV"
BASE COUNT          231 a          452 c          403 g          176 t
ORIGIN      
        1 cacaggattc gcaccggaga tggagagcgc ctccacaggg ggatcgagcg actccagctc
       61 caggggccac gtggcgacgg gaagcctgcg cacatacgca accttcggca gaagggcgaa
      121 aggaaagagc gccatctcgt ccttgctgaa ggcaagcccc gcgcccggcc ccgagaaggc
      181 ccccaggagg acggcgagcg ccccgatcca gcggaactgc gcatcgccgt gcagataccg
      241 ttgcgcgccg aaggctgcgt agtgcgtcac gggggtgaag cgcatcagcc actccagaag
      301 ctccgacgcg cactccgcgg agacgcccgc cgtacgaagc agaaaagccc cgtcgagcga
      361 ccgcgccgtg cgctcgaact cctccggatt ctcgacgcgc tcccgccacc cgtcgctcgc
      421 cccttcctcg aggagccgtg cgcagagcca cgccgccagc gcccagtcgc cgcccgggtt
      481 cacccgccac caccggtcgg cgaagcgcgt tgtcggcgtc tcgcgaatct ctaacgaggc
      541 gatctcgccc ccccggcctc gtacccgctg cagcagcggg acgacgtgcg cgtgcgtctc
      601 cagcgcgttc cgcccccaga agaggacgcc gcgcgcatgg tctgaaagcg actccggacg
      661 cgtgacgggc acttcgccga agctctccgc aaggccgaag ctccccgccg aagagcagag
      721 gtttcccttc ttcgtcgtgt acccgccgag cgcggcgaag aggtgcggca gcaggagctt
      781 cgagaagtag agcgagcctg cgctcgaaag gtgcatcaac gacagcggcc cgtgccggga
      841 gaccgccgaa cggaccttct ccgcccagac gtccaccgct tcgtcccagg agacggggac
      901 gagcgcgccc tccctgcgga gcatcggcgt cgtcagccgt tcggaactcg tcgccctcga
      961 agcccagcgc agccccttcc cgcacaggaa ggtggtgtac ggcagatccc tattcggctc
     1021 cagggtcacg gacgccccgt cgaacatgcc cttcatcgcg caggcatccg ggcagtcgta
     1081 gggacacgcg gtcgtttttc ggcgcatatc ggtcattgat gcatcctcct cgtcgttgtt
     1141 gtagaataat agcattgtcg gggcgcttcc gacaggcttt ctttgcggca ggaacggaaa
     1201 gagcgcccgg agaacatcac gcccggacgc cctgcgcgaa aaggacggcg gtcccgcctg
     1261 ga
//
LOCUS       DAOTIL010000919         1262 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminobacteriaceae bacterium isolate AMR_MDS_3026
            NODE_129757_length_1262_cov_2.121790, whole genome shotgun
            sequence.
ACCESSION   DAOTIL010000919 DAOTIL010000000
VERSION     DAOTIL010000919.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868050
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminobacteriaceae bacterium (wastewater metagenome)
  ORGANISM  Aminobacteriaceae bacterium
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae.
REFERENCE   1  (bases 1 to 1262)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1262)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:19:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,224
            CDSs (total)                      :: 3,198
            Genes (coding)                    :: 3,184
            CDSs (with protein)               :: 3,184
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1262
                     /organism="Aminobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_129757_length_1262_cov_2.121790"
                     /isolate="AMR_MDS_3026"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:3034609"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..210
                     /locus_tag="P5201_14510"
     CDS             <1..210
                     /locus_tag="P5201_14510"
                     /inference="COORDINATES: protein motif:HMM:NF014043.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF86 domain-containing protein"
                     /protein_id="HRV99808.1"
                     /translation="RSIEIIGEAAKKIPEEYRALHSDIEWRKFTGMRDRLIHDYFGVD
                     YRIVWDVVVNKLPGLREQLRRFVNT"
     gene            complement(537..755)
                     /locus_tag="P5201_14515"
     CDS             complement(537..755)
                     /locus_tag="P5201_14515"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicB family
                     antitoxin"
                     /protein_id="HRV99809.1"
                     /translation="MKAEFTAIIEAAPEGGYWAMCPEIPGANGQGETLEEAKESLREA
                     IYLILEDRREDVLRGLPDDVIRETVSVG"
BASE COUNT          284 a          350 c          335 g          293 t
ORIGIN      
        1 cgcagtatcg agataatcgg cgaggctgca aaaaagatac cggaagaata tcgcgcgctc
       61 cacagcgata tcgagtggag aaaattcacc ggtatgcgcg accggttgat tcacgactat
      121 tttggcgtag actatcggat cgtctgggac gttgtcgtca ataagctccc cggactgaga
      181 gaacagcttc gccgttttgt gaacacgtga gacttcgaca ggaggagtaa tcatcggtga
      241 atatcttcaa aggggagggg caaactgctt cccccctttc tttagaaagt ggaaacacca
      301 gcgaatcgga gcgtgctatg aacggctttt cggagatcga gatcccggaa tcgccattaa
      361 atctcacgcg tcgaggctct ttagtatttt cctcgccaac ggttctttga tctccgtatg
      421 ccgaggaaca gcttccatcg taccggtttt cgggttgatc cacagagcat gcgaagcgcc
      481 ctcgcgtttc aaataacacc ccgccatgcg aagcaagcgt tccaaatcct ttcgcttcat
      541 ccgaccgata ctgtttcgcg tatgacatcg tccggaaggc cgcgtagaac gtcctctctg
      601 cggtcctcca aaatcagata tatggcttcc cgcaggctct ctttcgcctc ttcgagcgtc
      661 tctccctgtc cgttggcgcc gggaatctcc gggcacatcg cccagtagcc tccctcgggc
      721 gccgcttcga taatcgcggt gaattcggct ttcatactct tccctcctcc tgcgatgcgc
      781 ggagcgccgc acgcggtttc gcatcgaatt atagcacctt gaagatacag gaatgatgta
      841 cataccgact ttcacgaagg agccctcgga gaagcgcagt ggcattctcc ggagaagcgt
      901 cgtctgattc gacaggagag ggatgctctt ccgcgaaggc aacgccgcgg aggtcgtacc
      961 tgtggagagc gctgaatgaa ccgatttcaa cgttccaaca tcgtgactcc gctctttttg
     1021 tcctgagacg atccgcctca tcctcccttt actgtcgcag gtgcgagaag gggattactc
     1081 ggcctcatca agcttcttgt cgaaaaacag ctccgtcacg gagcgagatc gggatcttga
     1141 cattgccatc ctgcttatag taggttactc gacgtacgag tgggcgtgtt tgaaagagag
     1201 attgctggaa gccaagcaac cgacggttat cgaagcggcg cagttggaag aacgcccgga
     1261 cc
//
LOCUS       DAOTIL010000920         1261 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminobacteriaceae bacterium isolate AMR_MDS_3026
            NODE_129861_length_1261_cov_2.569652, whole genome shotgun
            sequence.
ACCESSION   DAOTIL010000920 DAOTIL010000000
VERSION     DAOTIL010000920.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868050
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminobacteriaceae bacterium (wastewater metagenome)
  ORGANISM  Aminobacteriaceae bacterium
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae.
REFERENCE   1  (bases 1 to 1261)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1261)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:19:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,224
            CDSs (total)                      :: 3,198
            Genes (coding)                    :: 3,184
            CDSs (with protein)               :: 3,184
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1261
                     /organism="Aminobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_129861_length_1261_cov_2.569652"
                     /isolate="AMR_MDS_3026"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:3034609"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1261)
                     /locus_tag="P5201_14520"
     CDS             complement(<1..>1261)
                     /locus_tag="P5201_14520"
                     /inference="COORDINATES: protein motif:HMM:NF020926.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF1998 domain-containing protein"
                     /protein_id="HRV99810.1"
                     /translation="GYTHLERRAIEDKMAGGKLRGLVATNVLELGIDIGRLDTAVLVG
                     FPGTRASFWQQAGRAGRKGAKSSVFLLLDNLPLDQYLAINPEWLFSGESESAVVDPDN
                     LFIQLAHVRAAAAELPLSPDDLALFPDLGEIVPVLFSSKELRKEGGKFFWSGGVYPSG
                     DFSLRNMDRVRYKLKLGADGSLLTEMDELQAFREIYRGAIYMHEGRQYLVERMDTKNT
                     EALAKPVDGNYYTVSCDTTEVRKLQELKRSAAGRTTRRFGDVRVSYTTLGFRRQQFHN
                     HQNLGYEALERPLSKTFDTEGFWIRLPDEVRTLFLALTPQEENRPPMQFWKTYAEGLG
                     FALLNATMMATMTTHDDIGSALLADTDGEGGGMSVCVFDMYAGGLGFADKGYDRVSEI
                     IANAALMVEGCACTDGCAACVGDYHLD"
BASE COUNT          198 a          444 c          390 g          229 t
ORIGIN      
        1 ttgtccagat ggtagtcgcc cacgcacgcg gcgcagccgt ccgtacacgc gcacccctcg
       61 accatcagcg ccgcgttggc gatgatctcc gatacgcggt cgtacccctt gtccgcgaag
      121 cccagtcctc cggcgtacat gtcgaaaacg cagaccgaca tgccgcctcc ctctccgtcg
      181 gtgtccgcca gcagcgcgct gccgatgtcg tcgtgcgtcg tcatcgtcgc catcatcgtc
      241 gcgttgagca gggcgaaccc cagcccctcg gcgtacgtct tccagaactg cattggcggg
      301 cggttctcct cctgcggggt gagcgccagg aagagggtgc ggacctcgtc gggaaggcgg
      361 atccagaacc cctcggtatc gaacgtcttg ctcaacgggc gctccagcgc ctcgtacccc
      421 aggttctgat ggttgtggaa ctgctggcgg cggaagccga gcgtggtgta cgagacccgc
      481 acgtcgccga agcgccgcgt ggttctgccc gccgcgctcc gcttcagctc ctggagcttg
      541 cgcacctcgg tcgtgtcgca ggagaccgtg tagtagttcc cgtccaccgg cttcgccagc
      601 gcttcggtgt ttttcgtgtc catccgctcc acaaggtact gccgcccttc gtgcatgtag
      661 atcgcgcctc tgtagatctc gcggaacgcc tgcagttcgt ccatctccgt gagcagcgag
      721 ccgtcggcgc cgagcttcag cttgtagcgc accctgtcca tgttgcgcag gctgaagtcg
      781 ccggaggggt agacgccgcc cgaccagaag aacttccccc cctccttccg cagctccttc
      841 gacgagaaga gcaccggcac gatctccccc aaatccggga agagcgcgag atcgtccggc
      901 gagaggggga gttccgcggc ggcggcccgc acgtgggcga gctggatgaa gaggttgtcc
      961 gggtcgacga cggcgctttc gctctccccc gagaagagcc actccggatt gatcgcgagg
     1021 tactggtcca gcggcaggtt gtccagcaac aaaaaaacgg aggatttcgc ccccttccgt
     1081 cctgcgcgcc ccgcctgctg ccagaaggag gcgcgcgttc cggggaagcc cacgagcacg
     1141 gccgtgtcca gcctcccgat gtcgatgccc agctccagca cgttggtcgc cacaagcccg
     1201 cgcagtttac cgcccgccat cttgtcctcg atggcgcgcc gttccagatg cgtgtagccg
     1261 c
//
LOCUS       DAOTIL010000921         1258 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminobacteriaceae bacterium isolate AMR_MDS_3026
            NODE_130294_length_1258_cov_2.385702, whole genome shotgun
            sequence.
ACCESSION   DAOTIL010000921 DAOTIL010000000
VERSION     DAOTIL010000921.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868050
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminobacteriaceae bacterium (wastewater metagenome)
  ORGANISM  Aminobacteriaceae bacterium
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae.
REFERENCE   1  (bases 1 to 1258)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1258)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:19:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,224
            CDSs (total)                      :: 3,198
            Genes (coding)                    :: 3,184
            CDSs (with protein)               :: 3,184
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1258
                     /organism="Aminobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_130294_length_1258_cov_2.385702"
                     /isolate="AMR_MDS_3026"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:3034609"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..209)
                     /locus_tag="P5201_14525"
     CDS             complement(<1..209)
                     /locus_tag="P5201_14525"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRV99811.1"
                     /translation="MSKASQIVILCEDRAHELFAKRFLTKEWGVKHRAIRVLPYPNGM
                     GSGKKYVKDNLSREVEALRRRHAST"
     gene            complement(202..>1258)
                     /locus_tag="P5201_14530"
     CDS             complement(202..>1258)
                     /locus_tag="P5201_14530"
                     /inference="COORDINATES: protein motif:HMM:NF024700.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HRV99812.1"
                     /translation="GPGDFQLWLGDNGSGKTSVLNALRCVQRLMRGEHVEDIFNRNSL
                     TTWDTRRDQTIGLSLTIDGEPYAYEVTIEYAEQTGKQRIKREELLWKDSSFFLFEGQE
                     AHLHRIDRKTGRVEAGVVFPADWSRSVIPTVAKRDDNKPLIRFREELDKILLIHPIPL
                     VVKDAAVSESRFLSEHAENFAQWYRHLLQEQPSIGYAAKRHLEDVLPGFGQLSLRESG
                     ESRKLMATFRIEGKDRDFDFMNISDGQRQLIVLYTILEALRAGVFSTVLIDEPDNFVS
                     MREIQPWLDNLHDICDEHGRQALIISHHPEVVNKMARGTEFWFSRHAGAHVVVRPFPV
                     VENMPPAEVMARGWENE"
BASE COUNT          282 a          377 c          310 g          289 t
ORIGIN      
        1 atggtcgaag cgtgtcgtct gcgcaacgct tcgacctccc gggagagatt atccttcaca
       61 tacttcttgc ccgaccccat tccgttcgga tagggcaaga cccggattgc gcggtgtttc
      121 actccccatt ccttcgtcag aaagcgcttc gcgaaaagct catgcgccct gtcctcgcag
      181 aggatgacga tctgcgaagc cttactcatt ctcccaccct cgcgccatca cttccgccgg
      241 cggcatattc tccactacgg ggaacgggcg cacgacgaca tgcgcgcccg catgccgcga
      301 gaaccaaaac tccgtccctc tcgccatttt attcacaact tcggggtgat gagagatgat
      361 cagcgcctgc ctgccgtgtt cgtcacagat atcatgaagg ttatccagcc aaggttgaat
      421 ttcgcgcatc gaaacgaagt tgtccggttc gtcgatcagc acggtcgaaa acaccccggc
      481 gcgtaatgct tccaaaatcg tataaagaac aatcaactgg cgttggccgt cggaaatatt
      541 catgaaatcg aagtcgcggt ctttaccttc gatgcggaac gtcgccatca gtttgcgaga
      601 ttctccgctc tctcgaaggc tcaactgtcc gaagcccggc aacacatcct ccaaatgtcg
      661 tttcgccgca tacccgatag acggttgctc ttgtaagaga tggcggtacc actgggcgaa
      721 attctcggcg tgctcggaga gaaagcgcga ttcggaaacg gcggcgtctt taacgaccag
      781 cggaatagga tgaatcaata gaattttgtc gagttcttcc ctgaagcgaa tcaagggttt
      841 gttatcgtcg cgcttcgcga ccgtcggaat caccgaacgg gaccaatccg ccggaaaaac
      901 gacgccggct tccaccctgc ccgttttccg atcaatacga tgaagatgtg cctcctgccc
      961 ttcgaatagg aaaaaagaag agtctttcca gagcagctcc tcccgtttga tgcgctgctt
     1021 gcccgtctgt tccgcatatt cgatggtaac ttcgtatgcg tacggctcgc cgtctatcgt
     1081 cagagagagc cctatcgtct gatcccgcct tgtatcccag gttgtcagac tgttgcgatt
     1141 gaatatatcc tccacatgct ccccgcgcat caaacgttga acacagcgca acgcgttcag
     1201 caccgatgtt ttgcctgaac cgttgtcacc gagccagagt tgaaagtcgc ccggacca
//
LOCUS       DAOTIL010000922         1257 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminobacteriaceae bacterium isolate AMR_MDS_3026
            NODE_130400_length_1257_cov_4.105657, whole genome shotgun
            sequence.
ACCESSION   DAOTIL010000922 DAOTIL010000000
VERSION     DAOTIL010000922.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868050
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminobacteriaceae bacterium (wastewater metagenome)
  ORGANISM  Aminobacteriaceae bacterium
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae.
REFERENCE   1  (bases 1 to 1257)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1257)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:19:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,224
            CDSs (total)                      :: 3,198
            Genes (coding)                    :: 3,184
            CDSs (with protein)               :: 3,184
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1257
                     /organism="Aminobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_130400_length_1257_cov_4.105657"
                     /isolate="AMR_MDS_3026"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:3034609"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..978)
                     /locus_tag="P5201_14535"
     CDS             complement(<1..978)
                     /locus_tag="P5201_14535"
                     /inference="COORDINATES: protein motif:HMM:NF028510.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cache domain-containing protein"
                     /protein_id="HRV99813.1"
                     /translation="MKFNLKLNRLGARILAVVIVLLVVSVGGIGFYAMQVGGRGLQDA
                     ARQYETTLSENLAYKLNSTFNRFDGMLQALSALVTTRFTSMPNPEVADIVIPQFGAQN
                     GRFMNDLGVQSPEARSLFIIFNPDVFGTAKTHMVGFQRKDANTLFMYIDEKTIPAAEL
                     VDRKNPSTSWFWTPLDTKRPYWADVSRTEDGGEIVTYAMPVLIGEDIVAVAGMTFDFS
                     FVRETLKAVKIYDSGYPFLMNRNLKYLYHPTLSFDGPGFREVSNGYFAKYADDILNKG
                     KGRIDYPWENDQKTMSYYTMENGYILFVSASIAESLAAVGAMQRAVYIGI"
     regulatory      complement(1075..1159)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01051"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="cyclic di-GMP riboswitch class I; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /bound_moiety="cyclic di-GMP"
                     /db_xref="RFAM:RF01051"
BASE COUNT          232 a          342 c          389 g          294 t
ORIGIN      
        1 gatgcctatg tagacggcgc gctgcatcgc gccgaccgcg gcgagcgact cggcgatgct
       61 tgcggaaacg aagaggatgt agccgttctc catggtgtag tacgacatcg tcttctggtc
      121 gttctcccac ggatagtcga tccgcccttt gcccttattg aggatgtcgt cggcgtactt
      181 ggcgaagtaa ccgttcgata cttcccggaa gccggggccg tcgaacgaga gcgtcgggtg
      241 gtagaggtac ttgaggttgc ggttcatcag gaaggggtag ccgctgtcgt agatctttac
      301 ggctttgagc gtctcgcgga caaaggagaa atcgaaggtc atccccgcga cggcgacgat
      361 atcttccccg attaagaccg gcattgcgta cgtgacgatc tcccctccgt cttcagtacg
      421 gctgacgtcc gcccagtagg ggcgttttgt gtccaagggt gtccagaacc atgaagtcga
      481 gggatttttc cggtcgacaa gctccgcggc ggggatagtc ttttcgtcga tgtacatgaa
      541 gagggtattc gcgtccttgc gctggaaacc gaccatgtgc gtcttcgccg tgccgaagac
      601 gtccgggttg aagatgatga agaggctgcg cgcttcgggg gactggacgc cgaggtcgtt
      661 catgaacctg ccgttctgcg ccccgaactg cgggatcacg atgtcggcga cctcggggtt
      721 cggcatactg gtgaatcggg tcgttacaag tgcggagagg gcctgaagca tgccgtcgaa
      781 gcggttgaac gtggagttga gcttgtaggc gaggttctcc gagagcgtcg tttcgtactg
      841 ccgcgccgca tcctggagtc cgcggcctcc gacctgcatc gcataaaagc cgatgcctcc
      901 tactgaaaca accagcagga caatgactac tgcaagtatt cgtgcgccga gcctgttcag
      961 tttcagattg aatttcaacg caattcctcc ttttaaaaaa gtagactagg tcttacttgc
     1021 tctaaaaaca gcagcctccg gttttcggtt cggaggctgc gatgaaatac ttcctccggc
     1081 ggctcggcgg tcgttgcccg aaggcggtag acccgtagct ttgcgtcccc gtctttcgaa
     1141 gggtttgccc ttgttcggtt cagtgagaca gcgtatccgg cgttccttgg cggaaggcgc
     1201 cgggcgcctc ttgcttacag cttttcgcgc agcgccttca gctgttgcgc gcgttcc
//
LOCUS       DAOTIL010000923         1257 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminobacteriaceae bacterium isolate AMR_MDS_3026
            NODE_130418_length_1257_cov_3.350250, whole genome shotgun
            sequence.
ACCESSION   DAOTIL010000923 DAOTIL010000000
VERSION     DAOTIL010000923.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868050
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminobacteriaceae bacterium (wastewater metagenome)
  ORGANISM  Aminobacteriaceae bacterium
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae.
REFERENCE   1  (bases 1 to 1257)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1257)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:19:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,224
            CDSs (total)                      :: 3,198
            Genes (coding)                    :: 3,184
            CDSs (with protein)               :: 3,184
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1257
                     /organism="Aminobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_130418_length_1257_cov_3.350250"
                     /isolate="AMR_MDS_3026"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:3034609"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            191..>1257
                     /locus_tag="P5201_14540"
     CDS             191..>1257
                     /locus_tag="P5201_14540"
                     /inference="COORDINATES: protein motif:HMM:NF015724.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CsgG/HfaB family protein"
                     /protein_id="HRV99814.1"
                     /translation="MKKCFVLLAFCVLFVYSGAFAEAAEQKIRIGVIGFDSKAEGVSQ
                     EQADIVTDLFTRTLASSKTIAVYERERLAKVGEEIKLSMSGLVDMSTAIEVGKIAGVQ
                     YILMGSVTELSERMSGGAIPLFGLPVALGGGEHGAKATIDVRLIDTTTSETRLALSES
                     GTSTNSASAVSVMGLSFAEAEFGGLQARAIADAVNRLAHGIRSELGGESSHVLNVSGN
                     EVVIDVGSTMGAKEGQLYLVYAEGKTILGMSGESLGRDKMPLAVVKVRDVASGHSTCA
                     VASNCSLSLVRRGDKIEPVTAAKAKTMKFVSSRPAASSETFNQIFGPQGQSGAVASVP
                     QDAPAPAAETPAKPAVHEDKK"
BASE COUNT          274 a          316 c          415 g          252 t
ORIGIN      
        1 cgcagcggag cagggcgcgg cgaatcagac cgcaggggga gtggtgttgc cgatagtggg
       61 ctttggatca ggcaagaccg gcggtttgct cgccaccgcg acgcgcaact cggtgatcaa
      121 gcacgtcgaa acgatgaaga cggtgaactg ggaactgtag atgtttttta tttttcgggg
      181 gggttcaaat atgaagaaat gctttgtatt gctggctttt tgcgttctat tcgtttactc
      241 cggggctttc gccgaagctg cggaacagaa aatccgcatc ggcgtcatcg gcttcgacag
      301 caaggccgag ggggtttcgc aggagcaggc cgacatagta acggatctgt ttacccgtac
      361 tcttgcgagt tcaaaaacta tcgcggtcta tgagagggaa cgccttgcaa aagtcgggga
      421 ggagatcaag ctgagcatgt cgggactcgt cgacatgagc actgcgatcg aagttggaaa
      481 aattgcggga gtccagtaca tcttaatggg ttccgtgact gaactcagcg agaggatgtc
      541 ggggggagca attcctcttt ttggcctacc agtcgcgctc gggggcggag agcacggagc
      601 gaaggcgacc atcgacgtcc gccttatcga cacgacgacg agcgagaccc gccttgctct
      661 ttccgaaagc ggcacatcca cgaactcggc ttccgctgtc tccgtgatgg ggctttcgtt
      721 cgccgaagcg gagttcggcg gtcttcaggc gcgtgcgata gcggacgcgg tcaatcgtct
      781 ggctcacgga atccggtcgg aactcggagg cgagagttcc cacgtgctca acgtgtccgg
      841 gaacgaggtt gtcatcgatg tcggttcgac gatgggcgca aaggaagggc agctgtatct
      901 ggtttatgcc gaaggaaaaa cgattctcgg gatgagcgga gagtctttgg ggagagataa
      961 aatgccgctt gccgttgtga aagtacggga tgtcgcgagc ggacacagca cctgcgccgt
     1021 cgcttccaac tgcagtctga gcctggtgcg gcgcggcgac aagatcgagc cggtcactgc
     1081 ggcgaaggcg aagacgatga agttcgtctc cagcaggcct gcggcctcga gcgagacctt
     1141 taatcagatt ttcggccctc aaggccaaag cggagcggtt gcgtccgttc ctcaggatgc
     1201 cccggcgccg gcggcggaga caccagcaaa gcctgcggtg catgaagaca aaaaaga
//
LOCUS       DAOTIL010000924         1256 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminobacteriaceae bacterium isolate AMR_MDS_3026
            NODE_130567_length_1256_cov_3.820983, whole genome shotgun
            sequence.
ACCESSION   DAOTIL010000924 DAOTIL010000000
VERSION     DAOTIL010000924.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868050
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminobacteriaceae bacterium (wastewater metagenome)
  ORGANISM  Aminobacteriaceae bacterium
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae.
REFERENCE   1  (bases 1 to 1256)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1256)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:19:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,224
            CDSs (total)                      :: 3,198
            Genes (coding)                    :: 3,184
            CDSs (with protein)               :: 3,184
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1256
                     /organism="Aminobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_130567_length_1256_cov_3.820983"
                     /isolate="AMR_MDS_3026"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:3034609"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>1256
                     /locus_tag="P5201_14545"
     CDS             <1..>1256
                     /locus_tag="P5201_14545"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HRV99815.1"
                     /translation="VLRGDPGRLRQILVNLVGNAVKFTHEGSVDVCVSRAEERDEGVL
                     LRFSVKDTGIGIPEEKLGALFDKFSQADASTARKYGGAGLGLAISKQLAELMGGSVGA
                     TSTEGKGAEFWFTALFDRSMEGADDNALAPSSEAAGFKNAKNSRMSRPRFERRGIRIL
                     LAEDNLTNQQVAVGILKKFGLWTDVVADGEEALAALENVRYDLVLMDCQMPRMDGYEA
                     SRRIRAREGGARRVPVIAMTAHTMVGDREKCLDAGMDDYIAKPVSPEVVLDVLERWLP
                     AGESGVHEYVSVTEDARREAGAQPASPQAPFSPSAWDSSVLKERLMGDDDLMREVLLD
                     FLGDIPGKVRALENAVADEDAVAARSLAHAIKGAAGSVGGDGLQSAALEVEQLCMAGD
                     IEGAAAPMETLRREFGQLEYEMERFLS"
BASE COUNT          242 a          360 c          432 g          222 t
ORIGIN      
        1 gtacttcggg gcgaccccgg acgtctgcgc cagatcctcg tcaatcttgt cgggaacgcg
       61 gtcaaattca cccacgaagg aagcgtcgac gtctgcgtgt cgcgtgccga agaacgcgac
      121 gaaggcgtgc tgctccgttt ttcggtgaag gacaccggaa tcggcatccc tgaggagaag
      181 ctcggagccc tcttcgacaa gttcagccag gcggacgcct cgacggcacg gaagtacggc
      241 ggcgccgggc tggggctcgc catcagcaag cagcttgccg aactgatggg aggttccgtc
      301 ggtgcaacga gcacggaagg aaagggggcg gagttttggt tcaccgcgct ttttgatcgg
      361 agcatggaag gcgctgatga caatgcgctc gctccctctt cggaggccgc cggcttcaag
      421 aacgcgaaga actcccggat gtcgcgtccc cggtttgaac gaaggggaat ccgcatcctc
      481 cttgccgagg ataacctcac caatcagcag gtcgccgtgg gtatcctgaa aaaattcggc
      541 ctgtggacgg acgtcgtcgc ggacggcgaa gaggcgctcg ccgcattgga gaacgtgcgt
      601 tacgacctcg tgctgatgga ctgtcagatg ccccggatgg acggctacga agcgtcacgc
      661 agaattcgcg cgagggaggg cggcgcacgg cgcgttcccg tcatcgccat gacggcgcac
      721 acgatggtcg gagacaggga gaagtgtctg gacgccggaa tggacgacta tatcgcaaaa
      781 cccgtttctc cggaggttgt gctggacgtg ctcgaacggt ggttgcccgc cggagaaagc
      841 ggcgttcacg agtatgtgtc cgtcacggaa gacgcgaggc gcgaagcagg cgcgcaacct
      901 gcttccccgc aggcgccctt ttcgccttcg gcctgggact cctccgtcct caaagaacgc
      961 ctcatgggcg acgacgacct gatgcgcgag gtgcttttgg attttctggg cgatatcccc
     1021 ggaaaagtcc gcgctcttga gaacgcggtt gccgatgaag acgccgttgc tgcgcgaagt
     1081 ctggcgcacg ccatcaaagg ggctgcggga agcgtcggcg gcgacgggct ccagtccgcg
     1141 gcgctggagg tggagcagct ctgcatggct ggggatatcg agggcgcggc ggcgcctatg
     1201 gagacgctgc gacgcgagtt cggacaatta gagtacgaga tggagcgctt cctttc
//
LOCUS       DAOTIL010000925         1256 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminobacteriaceae bacterium isolate AMR_MDS_3026
            NODE_130574_length_1256_cov_3.550375, whole genome shotgun
            sequence.
ACCESSION   DAOTIL010000925 DAOTIL010000000
VERSION     DAOTIL010000925.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868050
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminobacteriaceae bacterium (wastewater metagenome)
  ORGANISM  Aminobacteriaceae bacterium
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae.
REFERENCE   1  (bases 1 to 1256)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1256)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:19:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,224
            CDSs (total)                      :: 3,198
            Genes (coding)                    :: 3,184
            CDSs (with protein)               :: 3,184
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1256
                     /organism="Aminobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_130574_length_1256_cov_3.550375"
                     /isolate="AMR_MDS_3026"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:3034609"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            72..1181
                     /locus_tag="P5201_14550"
     CDS             72..1181
                     /locus_tag="P5201_14550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008713128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HRV99816.1"
                     /translation="MKKIAPEILVIGGGAAGLSAAAEAASAGAKVLVVESDLRLGGQL
                     IKQTHKFFGSKDEYAGTRGYKIADILLNEIDSFKDSVSILQNTTVTGYYTEDDTFTAM
                     RGEEEVFQIKPQKTIVATGAQERLIPFPNNDLPGVYGAGAVQTLMNVYGVLPGKRVLM
                     VGAGNIGLIVSYQLMQAGVEVAAIVEAGPRIGGYWVHAAKIRRMGVPILLRHSIKKAL
                     GEDVITGAVITGLNEKFEQVGPEEEIECDLICIAVGLSPTTELLSQAGCKMMYVPQLC
                     GNVAQRDRTMRTSNPAFYTAGDASGIEEASAAMVEGRIAGLSAAAALGYPTEHGKIDE
                     YWSRLESLRMGEAGARICQGIECVLVKGWEGASHE"
BASE COUNT          343 a          311 c          347 g          255 t
ORIGIN      
        1 gccggaatga aagtcgaaac ccagcatggt aaagggaacg ttcccgactt aaacgacggg
       61 aggccgcgtt catgaaaaaa atagccccag aaatcctcgt aatcggtgga ggcgccgcag
      121 gactgagcgc agcggcagaa gccgcatcgg ccggagcgaa ggtattagtc gtcgaaagcg
      181 atcttcgcct cggaggccag ctcatcaaac agactcacaa gttcttcggc agtaaggatg
      241 aatatgccgg aacccgcggt tacaaaattg cagatatcct gctcaacgaa atcgattcct
      301 tcaaagacag cgtttctatc cttcaaaaca cgacagtcac aggttattac accgaagacg
      361 acaccttcac ggccatgcgg ggcgaggagg aagtttttca gataaaacca caaaaaacca
      421 ttgttgcaac tggagcgcaa gaacggctta ttcccttccc gaacaacgat cttcccggcg
      481 tttacggagc cggcgcggta cagactctca tgaatgttta cggtgttctg cccggaaaac
      541 gagtcctcat ggttggagca ggaaacatcg gccttatagt tagttatcaa ctgatgcagg
      601 caggcgttga agtcgccgcc atcgtcgaag ccggtccgcg tatcggaggc tattgggtcc
      661 acgcggcaaa aatacggcgc atgggcgtcc ctattctgct gcgccattca ataaagaagg
      721 cgttgggaga ggacgttatc accggcgcgg ttattacggg gctcaatgaa aaattcgaac
      781 aagtgggccc tgaagaagaa atagagtgcg acttgatttg catcgctgtc ggattgagcc
      841 ctaccacaga gcttctttcg caggccggat gtaaaatgat gtacgttccg cagctttgcg
      901 gaaatgttgc acaaagagac cgcacaatga gaacctcgaa cccggctttt tatactgccg
      961 gagacgccag cggaatcgaa gaagccagcg cagctatggt tgaaggacgc attgcaggtc
     1021 tttcagcagc agcagctctt ggttatccta ccgagcatgg caaaatcgac gaatactgga
     1081 gccgtcttga atcgctccga atgggcgaag ctggagcccg gatatgtcag ggtatcgaat
     1141 gcgttctcgt aaaaggctgg gagggtgcat ctcatgagtg acaaaaacat ggatatgcta
     1201 ttcaacagcg gagtcttgac cggagaaaga gaaggggcca tccttcctcc acgtga
//
LOCUS       DAOTIL010000926         1252 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminobacteriaceae bacterium isolate AMR_MDS_3026
            NODE_131224_length_1252_cov_2.934002, whole genome shotgun
            sequence.
ACCESSION   DAOTIL010000926 DAOTIL010000000
VERSION     DAOTIL010000926.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868050
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminobacteriaceae bacterium (wastewater metagenome)
  ORGANISM  Aminobacteriaceae bacterium
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae.
REFERENCE   1  (bases 1 to 1252)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1252)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:19:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,224
            CDSs (total)                      :: 3,198
            Genes (coding)                    :: 3,184
            CDSs (with protein)               :: 3,184
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1252
                     /organism="Aminobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_131224_length_1252_cov_2.934002"
                     /isolate="AMR_MDS_3026"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:3034609"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..842
                     /locus_tag="P5201_14555"
     CDS             <1..842
                     /locus_tag="P5201_14555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006299815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="molybdopterin-binding protein"
                     /protein_id="HRV99817.1"
                     /translation="RMAPGEVHEDDAALRIGRRVAGEGVALRGPEEGKCSLVAERDGL
                     LVYRDEDIDFVNDDPDWLFTTLPNKVRVGRGKTVAAFRIGPLMVREEQVARVEGIGPL
                     SVLPWRRLKTALVTTGRELYEGTVKDAFQPKLREKLAGYGGEFLGGRVVPDDRSAIQE
                     AISGWLDEGAELVLCTGGMSVDADDMTPSAIRAVCDSVLFRRTPVIPGANLMLAVKGG
                     AFVLGVPASAVFIERTSLDILLDRLFAGVPPDGKEVRGWGRGGLCAHCDSCGYPRCAF
                     AAR"
     gene            870..>1252
                     /locus_tag="P5201_14560"
     CDS             870..>1252
                     /locus_tag="P5201_14560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006299821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2Fe-2S iron-sulfur cluster-binding protein"
                     /protein_id="HRV99818.1"
                     /translation="MANKMITAVINGKTITAESGSTILQAARANNIYIPTLCDHPSLP
                     PSGACRVCLVEAERNPKLLTACTTPLTEGMVVSTNSPRVLSARKAVVEMLLIRHPLDC
                     FSCESNGRCELQNLAYELGIEKSPFT"
BASE COUNT          244 a          391 c          404 g          213 t
ORIGIN      
        1 ttcggatggc ccccggagaa gtccacgagg acgacgccgc gctccgcatc ggacggcgcg
       61 ttgccggaga gggcgttgcg ctgcgcggtc ccgaggaggg gaagtgctcg ctcgtcgcgg
      121 agcgcgacgg actgctcgtc tatcgcgacg aggatatcga cttcgtcaac gacgatcccg
      181 actggctttt cacgacgctg ccgaacaaag tgcgcgtcgg gaggggaaag accgtcgccg
      241 ctttcagaat agggccgctg atggtacggg aggagcaggt cgcccgcgtc gaaggcatag
      301 ggcctctatc ggtgcttccg tggcgccgtc tgaaaaccgc cctcgtgacc acggggcgcg
      361 agctgtacga gggaaccgtc aaggacgcgt tccaacccaa actgagggaa aagctcgccg
      421 ggtacggcgg agagtttctc ggcggccgag tcgtcccgga cgaccgttca gccatccagg
      481 aggcgatctc cggctggctg gacgaaggcg cggagcttgt gctctgtacc ggcggaatga
      541 gcgtggacgc cgacgacatg acgccctccg cgatccgcgc agtgtgcgac agcgtactgt
      601 ttcgaagaac gccggtgatt cccggggcca atctcatgct cgccgtcaaa ggcggcgcgt
      661 ttgtcctcgg cgttccggca tcggctgtgt tcatagaaag gacgtcgctc gacatcctcc
      721 ttgacaggct cttcgcgggc gttccgcccg acggcaaaga ggtcagaggc tggggaaggg
      781 ggggactgtg cgctcactgc gactcctgcg gctatccgcg atgcgccttc gcggcgcgat
      841 agcgagaaaa aagaaaagga gactgaacca tggcgaacaa aatgatcacc gctgtgatta
      901 acggaaaaac gatcacggcg gagtccgggt ccacgatcct ccaggcggcc cgggccaaca
      961 acatttatat cccgacgctc tgcgatcatc cgtctctgcc tccgagcggc gcgtgccgcg
     1021 tctgtcttgt cgaggcggag agaaacccga aactcctcac tgcctgcacg acgccgctta
     1081 ccgagggcat ggtcgtgtcc acgaacagcc ccagggttct ttcggcgcgc aaagccgtcg
     1141 tcgagatgct gctgatccgc catccgctcg actgcttctc ctgcgagtcc aacggcagat
     1201 gcgaactgca gaacctcgcc tacgaactcg gcatcgaaaa atcccccttc ac
//
LOCUS       DAOTIL010000927         1246 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminobacteriaceae bacterium isolate AMR_MDS_3026
            NODE_132123_length_1246_cov_3.832914, whole genome shotgun
            sequence.
ACCESSION   DAOTIL010000927 DAOTIL010000000
VERSION     DAOTIL010000927.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868050
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminobacteriaceae bacterium (wastewater metagenome)
  ORGANISM  Aminobacteriaceae bacterium
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae.
REFERENCE   1  (bases 1 to 1246)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1246)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:19:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,224
            CDSs (total)                      :: 3,198
            Genes (coding)                    :: 3,184
            CDSs (with protein)               :: 3,184
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1246
                     /organism="Aminobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_132123_length_1246_cov_3.832914"
                     /isolate="AMR_MDS_3026"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:3034609"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1246)
                     /locus_tag="P5201_14565"
     CDS             complement(<1..>1246)
                     /locus_tag="P5201_14565"
                     /inference="COORDINATES: protein motif:HMM:TIGR01494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="HAD-IC family P-type ATPase"
                     /protein_id="HRV99819.1"
                     /translation="AQCSARIEESVRLLPGVSFAALDLVAGRLRVVLSGEHNGDETLS
                     RIRGIVDSIEPGVSVSEEGAQAKSVSLPLKEIARLSAGVLLWVAAMFAEVSEGVRMAL
                     YVAAYLAAGINVLRTVFGNLRQGRIFDEFFLMTIATGGAFAIGEFSEAVAVMLFYESG
                     ELVQDLAVAKSRRSILSLMDIRPDSAVLLEEGGERRVRAEDVRVGETVLVKPGERIPL
                     DGSVKTGTSSLDTSSLTGESVPRDVAPGDDVLAGSVNLAGTLTVVVSRLFGESTLSKG
                     LRLIEEARERKAPTERFITAFAKRYTPAVVAAAALLAVVPPLMGLGAFREWGYRALIF
                     LVVSCPCALVISVPLALFGGIGAASRRGVLLKGGNVLETLAKVRTVLFDKTGTLTRGV
                     FRVTGVFQAGNTDRERLLSIAG"
BASE COUNT          273 a          434 c          335 g          204 t
ORIGIN      
        1 tccccggcga tcgaaagcaa tctctcccgg tccgtattcc cggcctggaa gactccggtc
       61 accctgaaca cgcccctagt caacgtaccc gtcttgtcga aaagcacggt gcggactttc
      121 gccagcgtct ccaacacatt tccacctttc aaaagcacgc ctcgccttga agcggctccg
      181 ataccgccga agagcgcgag cggcacggag atcacaaggg cgcacggaca ggagacgacg
      241 aggaagatga gcgcgcggta cccccattcc cggaacgcgc ccaaccccat cagcggcggc
      301 acgacggcga gcagcgcggc ggcggcaacc accgccgggg tatatctttt tgcaaaggcg
      361 gtaatgaagc gctctgtggg cgcctttcgt tcccttgcct cttcaataag gcgcaaccct
      421 ttcgagagcg tggactcgcc gaagagacgg ctcacaacga ccgtgagcgt tccggccagg
      481 ttgacgcttc cggcaagcac gtcgtcgccg ggcgcaacat cccgcggaac ggattcgccc
      541 gtcaacgacg acgtatccag cgatgatgtc cccgttttca ctgaaccgtc gagaggaatt
      601 cgttcgcctg gcttcacaag gacggtctcc cccacccgga cgtcctccgc acgcacgcgc
      661 cgctctcccc cttcttcgag aagcactgcc gaatcgggac ggatatccat cagcgagaga
      721 atggaacgtc gggacttcgc caccgccaaa tcctgcacaa gttcccccga ctcgtaaaag
      781 agcatcaccg ccacggcctc ggaaaactcg ccgatggcga acgctccgcc tgtggcaatc
      841 gtcatcagga agaactcgtc aaagattctc ccctgccgca gattgccgaa cacagtacgg
      901 agcacgttga tgcccgccgc aaggtaggca gccacataga gtgccatccg caccccttcg
      961 gaaacctccg caaacatcgc cgcaacccag agcagcaccc ctgccgaaag acgcgcgatt
     1021 tccttcagcg gaagagagac gcttttcgct tgcgccccct cttccgagac ggagactccc
     1081 ggttctatcg aatctacaat tccgcggatg cgcgataagg tttcgtcgcc gttgtgctca
     1141 ccggacaaga ccactctgag ccgcccggcg accagatcaa gcgctgcgaa cgacacgccg
     1201 ggaagcaggc gaacggactc ctctatcctc gcggaacatt gggcgc
//
LOCUS       DAOTIL010000928         1246 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminobacteriaceae bacterium isolate AMR_MDS_3026
            NODE_132124_length_1246_cov_3.780856, whole genome shotgun
            sequence.
ACCESSION   DAOTIL010000928 DAOTIL010000000
VERSION     DAOTIL010000928.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868050
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminobacteriaceae bacterium (wastewater metagenome)
  ORGANISM  Aminobacteriaceae bacterium
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae.
REFERENCE   1  (bases 1 to 1246)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1246)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:19:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,224
            CDSs (total)                      :: 3,198
            Genes (coding)                    :: 3,184
            CDSs (with protein)               :: 3,184
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1246
                     /organism="Aminobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_132124_length_1246_cov_3.780856"
                     /isolate="AMR_MDS_3026"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:3034609"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1214)
                     /locus_tag="P5201_14570"
     CDS             complement(<1..1214)
                     /locus_tag="P5201_14570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006301125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit B"
                     /protein_id="HRV99820.1"
                     /translation="MLPKEYKTISELSGPLLLVNKTKNVRYDELVEIELASGERRRGR
                     VLEITSDKALVQVFEGTDGIDIKSTKMLFLGKVLTLPVSKDMLGRVFNGRGEPIDDGA
                     PILAEKKLDINGMPMNPFSRDYPSEFIQTGISTIDGMNPMVRGQKLPIFSGSGLPHNR
                     MAAQIARQATVISGHEDFAVVFAAMGITFEEASFFMEDFRKTGAIQRTVMFVNLADDP
                     AIERITTPRLALTAAEYLAFEQDMHVVVILTDLTNYCEALREISAARKEVPGRRGYPG
                     YLYTDLATMYERAGRLKGKKGSITQIPILTMPEDDKTHPIPDLTGYITEGQIILSRTL
                     HRKGIYPPVDVMPSLSRLKDKGIGAGKTREDHADLMNQLFAGYARGKEAKELAVILGE
                     GALTEEDKAFAK"
BASE COUNT          249 a          337 c          347 g          313 t
ORIGIN      
        1 aatttcgcga acgccttatc ctcttctgta agagcgcctt cgccaaggat gactgcaagt
       61 tccttcgctt ctttcccgcg cgcatatccg gcaaagagct ggttcatgag gtccgcgtga
      121 tcttcccgcg tcttgccggc gccgattccc ttatctttca gtcgggacag agaaggcata
      181 acgtccacgg ggggatagat accttttctg tggagagtac ggctgaggat gatctgacct
      241 tcggtgatgt atccggtcaa gtcgggtatt ggatgcgtct tgtcgtcctc gggcatggtg
      301 aggatgggaa tctgcgtgat ggaacccttt ttccccttca gacgtccggc gcgctcgtac
      361 attgtggcaa ggtcggtgta gaggtagccg ggatagcctc tgcggccggg gacttccttg
      421 cgcgccgcag agatctcgcg caacgcttcg cagtagttag taaggtcggt caagatgacc
      481 acgacgtgca tgtcctgctc aaaagcaagg tattccgcgg cggtaagcgc aagtctaggc
      541 gttgtgatac gctcgatcgc ggggtcgtcg gccaggttga cgaacataac cgtccgttga
      601 atggctcctg tcttcctgaa gtcctccatg aagaatgaag cttcctcaaa tgtgattccc
      661 atcgcggcga agacgaccgc gaagtcctcg tgtccgctga tgacagtggc ttgacgggcg
      721 atctgcgcgg ccattctgtt atgcggtagg ccgctgccgg agaaaatcgg cagcttctgg
      781 ccgcgaacca ttggattcat tccgtcgatc gtcgagatac ccgtctgaat gaactcggaa
      841 ggatagtctc ttgagaacgg gttcatcggc ataccgttga tatccagctt tttctctgcg
      901 agaataggag ctccatcgtc aataggctcg ccgcggccat taaagactct gccgagcata
      961 tccttgctta cgggaagagt gagcaccttg ccaagaaaga gcatcttggt gcttttaata
     1021 tcgatgccgt cggttccttc aaacacctgc acaagcgcct tgtccgaggt gatctccaga
     1081 actcttccac ggcggcgttc accgcttgcg agttctattt ctaccaactc gtcgtaacgg
     1141 acgtttttcg ttttatttac cagcagaagc ggacccgaaa gttcgctgat ggttttatac
     1201 tccttaggca gcatcgcttt cacctcccac gggagtgact ccggcg
//
LOCUS       DAOTIL010000929         1243 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminobacteriaceae bacterium isolate AMR_MDS_3026
            NODE_132694_length_1243_cov_1.744949, whole genome shotgun
            sequence.
ACCESSION   DAOTIL010000929 DAOTIL010000000
VERSION     DAOTIL010000929.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868050
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminobacteriaceae bacterium (wastewater metagenome)
  ORGANISM  Aminobacteriaceae bacterium
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae.
REFERENCE   1  (bases 1 to 1243)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1243)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:19:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,224
            CDSs (total)                      :: 3,198
            Genes (coding)                    :: 3,184
            CDSs (with protein)               :: 3,184
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1243
                     /organism="Aminobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_132694_length_1243_cov_1.744949"
                     /isolate="AMR_MDS_3026"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:3034609"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..528)
                     /locus_tag="P5201_14575"
     CDS             complement(<1..528)
                     /locus_tag="P5201_14575"
                     /inference="COORDINATES: protein motif:HMM:NF013707.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016903 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoacid:acceptor oxidoreductase family
                     protein"
                     /protein_id="HRV99821.1"
                     /translation="MNARDEFIGIVLAGVGGQGLVTIGTILGEAAVLEGRNACLSSTY
                     GTEARGTFTKSDVLIGDGEIDFIEVVEPDVLLCLAQTAFDRSTPRLEAEGAERILLLY
                     DGDLVTPSPLCRAKSVGIPFARLASEQGVPGAANLAALGAVVGLTCAVRPESVRAVLE
                     ERAFRQGSAEAFDEGL"
     gene            complement(525..>1243)
                     /locus_tag="P5201_14580"
     CDS             complement(525..>1243)
                     /locus_tag="P5201_14580"
                     /inference="COORDINATES: protein motif:HMM:NF014794.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent enzyme"
                     /protein_id="HRV99822.1"
                     /translation="DDYIDVNAFHGTHGRALAFATGIKLANPELTVIVLMGDGDGATI
                     GGNHLIHAARRNVDLTAVVVNNFNYGMTGGQYSGTTPAGSKTSTSPLGHAEDPFDLCA
                     LAAAAGAPYVARGSVRDGAALRRLIRNGLSRRGFSFIEALSICPTHYGRRNDARTPFE
                     LYQKLGDMTLPLEKSASVSEEERHGKLLLGTFADKAPDDWTARYKQATKREIFPVCVD
                     RLGRHDSGRTQQATRKGERS"
BASE COUNT          182 a          446 c          408 g          207 t
ORIGIN      
        1 gagcccttca tcgaatgcct ccgcgctccc ctgcctgaac gccctttctt cgaggacggc
       61 gcgtaccgac tccggacgca ccgcgcacgt cagcccgacg acggctccca gagccgcaag
      121 gttggccgcg cccggaacgc cctgctccga cgcgaggcgc gcgaagggga tgccgacgct
      181 tttcgcccgg cagagcggcg aaggcgtcac cagatcgccg tcgtagagca gcagaatgcg
      241 ttcggccccc tccgcctcca gccggggggt ggagcgatcg aaggccgtct gcgccaggca
      301 gagcagcacg tccggctcga cgacctcgat gaagtcgatc tcgccgtcgc cgatgaggac
      361 gtccgacttc gtaaacgtcc cccgcgcctc ggtgccgtag gtcgaggaga ggcaggcgtt
      421 ccgcccttcg aggaccgccg cctcgccgag aatcgtcccg atggtgacca gcccctgccc
      481 gccgaccccg gcgagcacga tgccgatgaa ctcgtcccgc gcgttcatga ccgctccccc
      541 ttccgcgtcg cctgctgtgt gcggccagaa tcatgccgcc ctaaacgatc gacgcaaacg
      601 gggaaaatct cccgtttggt cgcctgctta tatctagctg tccagtcgtc gggcgctttg
      661 tcggcgaacg tgcccaggag gagctttccg tgccgctctt cctcgctcac ggacgcgctt
      721 ttttccagcg ggagcgtcat gtcgcccagc ttttggtaca gctcgaacgg cgtccgcgcg
      781 tcgttccggc ggccgtagtg cgtgggacag atggagagcg cttcgatgaa cgagaatccc
      841 ctccgcgaga gcccgttccg gatgagccgc cgcagcgccg ccccgtcgcg caccgagccc
      901 cgcgccacgt agggggctcc cgcagcggcg gcgagcgcgc agagatcgaa cgggtcctcc
      961 gcgtgcccca gcggcgacgt ggaggttttg ctccccgccg gcgtcgtgcc ggagtactgc
     1021 ccccccgtca tgccgtagtt gaagttgttc accaccaccg cggtgaggtc gacgttccgg
     1081 cgcgccgcgt ggatcaggtg gttgccgccg atggtcgcgc cgtcgccgtc ccccatgagc
     1141 acgatgacgg tcagctcggg gttcgccagc ttgattccgg tggcgaaggc cagcgcgcgc
     1201 ccgtgcgtgc cgtggaaggc gttcacgtcg atgtagtcgt cgg
//
LOCUS       DAOTIL010000930         1242 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminobacteriaceae bacterium isolate AMR_MDS_3026
            NODE_132727_length_1242_cov_4.235046, whole genome shotgun
            sequence.
ACCESSION   DAOTIL010000930 DAOTIL010000000
VERSION     DAOTIL010000930.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868050
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminobacteriaceae bacterium (wastewater metagenome)
  ORGANISM  Aminobacteriaceae bacterium
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae.
REFERENCE   1  (bases 1 to 1242)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1242)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:19:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,224
            CDSs (total)                      :: 3,198
            Genes (coding)                    :: 3,184
            CDSs (with protein)               :: 3,184
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1242
                     /organism="Aminobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_132727_length_1242_cov_4.235046"
                     /isolate="AMR_MDS_3026"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:3034609"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1065
                     /locus_tag="P5201_14585"
     CDS             <1..1065
                     /locus_tag="P5201_14585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008520278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IMP dehydrogenase"
                     /protein_id="HRV99823.1"
                     /translation="ITNRDLRFVQNFDQPISRIMTKDNLVTASEGTTLEGAQSILMKY
                     KIEKLPIVDENGILKGLITIKDIQKAKDFPNAAKDSGGRLRVGAAVGVGADLFERAAA
                     LVKSGVDVLVVDTAHGHAKKVLDSVKNLRGAYPEMTIIGGNIATAGAAEALIDAGADA
                     VKVGVGPGSICTTRIIAGIGVPQLGAIYNVALAAHARGKTVIADGGIRYSGDIVKAIA
                     AGADSVMIGSLFAGTEESPGEPIIYRGRSYKSYRGMGSLGAMKEGCSKDRYFQESAVG
                     DKLVPEGIEGLAAHKGSLSGVVFQLTGGLRSGMGYVGARDVQELQQKARFIKVTAASV
                     KENHPHDVVVTKEAPNYWVD"
BASE COUNT          275 a          304 c          408 g          255 t
ORIGIN      
        1 atcaccaaca gggatctgcg ttttgtccag aatttcgacc agcctatcag ccggattatg
       61 acgaaggaca atcttgtgac tgcgtcggaa ggaacaacgc tggagggagc gcagtccatt
      121 ctgatgaagt acaagataga gaagttgccg attgtggatg agaacggcat tctgaaaggc
      181 cttatcacga taaaggatat ccagaaggcg aaggatttcc cgaatgctgc gaaggacagc
      241 ggaggacgtc ttcgagtcgg cgcggctgtc ggagtcgggg cggatctttt tgaacgggcc
      301 gccgctctcg tgaagagcgg cgtcgacgtg ctcgttgtcg atactgcgca cgggcatgca
      361 aaaaaggtgc tcgattccgt gaagaacctc cggggagcgt acccggagat gacgatcatc
      421 ggaggaaaca tcgccaccgc cggagccgcc gaggccctca tcgacgccgg agcggatgct
      481 gtcaaggtcg gggtcggtcc cgggagcatc tgcacaacgc gaatcatcgc cggtatcggc
      541 gtcccgcagc tgggagcaat ttacaatgtc gctctggcag cgcacgctcg cggaaagacc
      601 gtcattgccg acggcggaat ccgctactcc ggagatattg tgaaagccat cgcggcaggc
      661 gccgattccg tcatgatcgg ttctcttttt gcgggcaccg aggagagtcc gggagaaccc
      721 atcatctaca gggggcgctc ctataagagt taccgcggca tggggtcgct cggcgcgatg
      781 aaagaagggt gcagcaagga ccggtacttc caggaaagcg cggttggtga taaacttgtt
      841 ccggagggta ttgaggggtt agcggcgcac aaaggttctc tctcaggcgt tgtcttccag
      901 ctgactggag gcttgcgctc cggtatgggg tatgtcggtg cgcgggatgt tcaggaactt
      961 cagcaaaaag cgcgttttat caaggtaacg gcggcctctg tgaaggagaa tcaccctcac
     1021 gacgtcgtgg tgacgaagga agcgccgaat tattgggtgg attgatgggg taggcctgct
     1081 cttcccgaga acgggggtgc gatatggcgg gagcaaaggt ttttgaacgg aatccgtacg
     1141 aggatgtcgt ctactggccc ggggtaattg ctcttcccga attaatggag gatgctatgg
     1201 ctgatgacct tctctttgcc gacgacccca tggagttcga ga
//
LOCUS       DAOTIL010000931         1239 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminobacteriaceae bacterium isolate AMR_MDS_3026
            NODE_133320_length_1239_cov_1.935811, whole genome shotgun
            sequence.
ACCESSION   DAOTIL010000931 DAOTIL010000000
VERSION     DAOTIL010000931.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868050
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminobacteriaceae bacterium (wastewater metagenome)
  ORGANISM  Aminobacteriaceae bacterium
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae.
REFERENCE   1  (bases 1 to 1239)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1239)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:19:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,224
            CDSs (total)                      :: 3,198
            Genes (coding)                    :: 3,184
            CDSs (with protein)               :: 3,184
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1239
                     /organism="Aminobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_133320_length_1239_cov_1.935811"
                     /isolate="AMR_MDS_3026"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:3034609"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..735)
                     /locus_tag="P5201_14590"
     CDS             complement(<1..735)
                     /locus_tag="P5201_14590"
                     /inference="COORDINATES: protein motif:HMM:NF013696.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HRV99824.1"
                     /translation="MGITLNVEQYLKDLEYLVNIDSQSKDPEGVAKVAAFFEKAFGDI
                     GWTVERVVFDPTAGPSLKITNGEPPYDALLLGHLDTVFPKGTVAERPFSRDEKRAYGP
                     GVNDMKSGLLFGLYAARALTAKGSKGSFCIAYNSEEEIGSRRARPWIEQLAQESRTSV
                     ILEPARPNGNLMNARKGLGRIEVTFNGKAAHAGVEPEKGVSAVNEMAYWIIALHGLTD
                     FEKGTTLNAGIVAGGTGANVVPEKATM"
     gene            complement(749..>1239)
                     /gene="zupT"
                     /locus_tag="P5201_14595"
     CDS             complement(749..>1239)
                     /gene="zupT"
                     /locus_tag="P5201_14595"
                     /inference="COORDINATES: protein motif:HMM:NF003243.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="zinc transporter ZupT"
                     /protein_id="HRV99825.1"
                     /translation="LIEDSEQKPQNNKRLIRMGILTALAIAIHNFPEGLATFTAALTD
                     PNLGIAIAVAIAIHNIPEGIAVSVPIYYATGSRRKAFNYSFLSGVSEPIGALLAWLVL
                     APFLSGALFGVIFASVAGIMVFISVDQLLPAARKYGEHHVSIYGLVIGMMIMAASLLL
                     FI"
BASE COUNT          208 a          387 c          393 g          251 t
ORIGIN      
        1 catggtcgcc ttctccggga cgacgttcgc gcccgtaccc ccggcgacga tgcccgcgtt
       61 gagcgtcgtc cccttctcga agtcggtgag cccgtggagc gcgatgatcc agtacgccat
      121 ctcgttgacc gcgctgaccc ccttttccgg ctccactccg gcgtgcgcgg cctttccgtt
      181 gaatgtcacc tcgatgcgcc cgagcccctt gcgggcgttc atcaggttgc cgttgggccg
      241 ggccggttcg agaatgacgg aggtgcggct ctcctgcgcc aactgctcga tccaagggcg
      301 cgcgcgtcgg gagccgatct cctcttcgct gttgtaggcg atgcagaacg accccttgct
      361 tcccttcgct gtgagcgccc gagcggcgta gaggccgaag agcagccccg atttcatgtc
      421 gttgacgccc ggtccgtagg cgcgcttttc atcccgcgag aacggacgct cggccacggt
      481 tcccttcggg aagaccgtgt ccaggtgccc cagcagcagc gcgtcgtacg gaggttcccc
      541 gttggtgatc ttcaggctcg gccctgcggt cgggtcgaaa accacccgtt cgacggtcca
      601 gccgatgtcg ccgaacgcct tctcgaagaa cgcggcgacc ttcgcgactc cctcgggatc
      661 cttcgactgg ctgtcgatgt tcacgaggta ttcaagatct ttcagatact gttcgacgtt
      721 gagcgttatc cccatgcaaa ctcccccttt atataaaaag cagcaggctc gcggccatga
      781 tcatcatgcc gataacgagc ccgtagatgg aaacgtgatg ttcgccgtac ttgcgggcgg
      841 ccggaagcag ctggtccacc gaaatgaaga ccatgatccc cgcgacggag gcgaagatga
      901 cgccgaagag cgctccgctc aggaagggcg ccagaacgag ccacgccagc agcgcgccga
      961 ttggttccga gactccggag aggaaggagt agttgaacgc ctttcttctg ctccccgtgg
     1021 cgtagtagat gggcaccgag acggcgatcc cctcggggat gttgtggatg gcgatggcga
     1081 cggcgatggc aatccccagg ttcgggtcgg ttagcgcggc ggtgaaggtg gccagcccct
     1141 cggggaagtt gtggatggcg atggccagag ccgttaggat ccccatgcgg attagccgct
     1201 tgttgttttg gggtttttgc tccgagtcct caatgagct
//
LOCUS       DAOTIL010000932         1239 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminobacteriaceae bacterium isolate AMR_MDS_3026
            NODE_133325_length_1239_cov_1.836149, whole genome shotgun
            sequence.
ACCESSION   DAOTIL010000932 DAOTIL010000000
VERSION     DAOTIL010000932.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868050
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminobacteriaceae bacterium (wastewater metagenome)
  ORGANISM  Aminobacteriaceae bacterium
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae.
REFERENCE   1  (bases 1 to 1239)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1239)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:19:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,224
            CDSs (total)                      :: 3,198
            Genes (coding)                    :: 3,184
            CDSs (with protein)               :: 3,184
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1239
                     /organism="Aminobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_133325_length_1239_cov_1.836149"
                     /isolate="AMR_MDS_3026"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:3034609"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            342..>1239
                     /locus_tag="P5201_14600"
     CDS             342..>1239
                     /locus_tag="P5201_14600"
                     /inference="COORDINATES: protein motif:HMM:NF012515.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PfkB family carbohydrate kinase"
                     /protein_id="HRV99826.1"
                     /translation="MILHSEKFKDIRIAVVGDVVLDKYVSGIARRISSEAAIPVLLAR
                     RIGHVAGGAANVAANLSGIGCSVSVYGAVGDDGNSVLLSELLDEKKIEHKLQQINGRP
                     TTIKTRIVAGNQQIARYDIEESSALSKHDEDLLFSSLLEEMDGLDAIILSDYGNGVLK
                     GSLCSRIIGTAATKGTPVFVDPRGNNWNRYRGAACATPNEAELALVSEGAEIDEQTLS
                     LHAHSARKRYGLRNLLVTRGAKGMALFLEDGEEFFLPAFCVREVFDVSGAGDTVIAVL
                     AASLIAGATWKQAADWANRAAGV"
BASE COUNT          285 a          343 c          374 g          237 t
ORIGIN      
        1 tacatggctt cgggggtagc tctccttgag cgccctgcaa taaggcgtaa ctatcagaat
       61 atcgccgagc gcactgtatc tcagaaagag aatgcgttcc ccttcgccgt ctttcgaagg
      121 gggggtgtag gtcctgcaag aacggatcat gcacggaacc tggaacaaaa aaacgaatga
      181 agaacctcca cggaacagtc actccttcga taacgccacg tagaaagaag cggcgggaaa
      241 aatgcacacc gcttcgtatt ctatcaaaga tattgtaaaa tggagcctga aaaccgaacg
      301 tgaatttgcc catctttcga tacttcaagg agcgagtcgc aatgatactc catagtgaaa
      361 agttcaagga cattcgtatc gccgtcgtgg gggacgttgt tctcgataag tacgtcagcg
      421 gaatcgcgcg gcgtatatcc tccgaggccg ccattccggt gcttctcgct cgaagaatag
      481 gtcatgtcgc gggcggagcg gcgaacgtcg cggcgaacct gtcggggatc gggtgttcgg
      541 tctccgtgta cggcgctgtc ggcgacgacg ggaacagcgt cctgctgagc gagctcctcg
      601 atgaaaaaaa gatagagcac aagctgcagc agatcaacgg ccgccccacg acgattaaaa
      661 cccgcatcgt tgccggaaat cagcagatcg cacgctatga tatcgaagag tcctcggcgc
      721 tctcgaaaca cgacgaagac ctgctctttt catccctgct ggaagaaatg gacggtctcg
      781 acgccattat cctctccgat tacgggaacg gcgttttgaa aggttctctc tgctcaagaa
      841 tcatcgggac ggcggcgaca aagggaacgc ccgtcttcgt cgaccccagg gggaacaact
      901 ggaaccgcta tcggggcgcc gcctgcgcca cgccgaacga ggcggagctg gccttggtct
      961 ccgagggcgc ggagatcgac gaacagacgc tctcgcttca tgcgcattcg gcacggaaac
     1021 gctacgggct ccgaaatctg ctggtgacgc gaggggccaa aggaatggca ctgttcctcg
     1081 aggacgggga agagttcttc cttcccgcgt tttgcgtgcg cgaggtgttc gacgtttccg
     1141 gagcgggcga tacggtcatc gccgttcttg cggcatcgct gatcgcgggg gcgacctgga
     1201 agcaggccgc cgactgggcg aaccgggccg ccggcgtcg
//
LOCUS       DAOTIL010000933         1238 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminobacteriaceae bacterium isolate AMR_MDS_3026
            NODE_133466_length_1238_cov_2.257819, whole genome shotgun
            sequence.
ACCESSION   DAOTIL010000933 DAOTIL010000000
VERSION     DAOTIL010000933.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868050
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminobacteriaceae bacterium (wastewater metagenome)
  ORGANISM  Aminobacteriaceae bacterium
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae.
REFERENCE   1  (bases 1 to 1238)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1238)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:19:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,224
            CDSs (total)                      :: 3,198
            Genes (coding)                    :: 3,184
            CDSs (with protein)               :: 3,184
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1238
                     /organism="Aminobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_133466_length_1238_cov_2.257819"
                     /isolate="AMR_MDS_3026"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:3034609"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..614)
                     /locus_tag="P5201_14605"
     CDS             complement(<1..614)
                     /locus_tag="P5201_14605"
                     /inference="COORDINATES: protein motif:HMM:NF023583.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008170 - N-methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006306 - DNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-6 DNA methylase"
                     /protein_id="HRV99827.1"
                     /translation="MFEQTFRNIDDLLRKEAGCTTELDYTEQSSWLLFLKYLEGLEQD
                     RADEALLSGKPYAYILDAPYRWESWAAPKGKDGQLDRNRAMTGDDLRDFVNQKLFPYL
                     HGFKSRATGPNTVEYKIGEIFGEIKNKIQSGYNLREIIDYVDELKFRSQVEKHELSAL
                     YEEKIQRMGNAGRNGGEYYTPRPLIRAIVEVVDPKIGERVYDGA"
     gene            complement(787..>1238)
                     /locus_tag="P5201_14610"
     CDS             complement(787..>1238)
                     /locus_tag="P5201_14610"
                     /inference="COORDINATES: protein motif:HMM:NF020476.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF1848 family protein"
                     /protein_id="HRV99828.1"
                     /translation="RKLRGKGIDEVGGEDMRTIARGFPKIAQAYGIAVQSCCEAVDFG
                     AEGCFPGGCIDAALAGELSGKPVPEKKDAGQRPDCRCAPSVDIGAYDTCPAGCAYCYA
                     TQSAAAVRANASRHDPASPLLSGTPSPEEQQLIERSRTPQQRRLALF"
BASE COUNT          208 a          378 c          370 g          282 t
ORIGIN      
        1 caggctccgt catagacgcg ttctccgatc ttggggtcga ctacttcgac gatggcgcgg
       61 atcagcggac gcggcgtgta gtactcgccg ccgttgcgac cggcgttccc catgcgctgg
      121 atcttttctt cgtacagcgc cgagagttcg tgcttttcca cttgggagcg aaacttcagt
      181 tcgtcgacgt aatcgatgat ctccctgaga ttgtagccgc tctgaatttt gttcttgatc
      241 tcgccgaaga tctcgccgat cttgtactcc accgtgttgg gaccggtggc gcggctttta
      301 aatccgtgca gatagggaaa gagcttctga ttcacgaagt ctcgcagatc gtctccggtc
      361 atggcccggt tgcggtcgag ctgcccgtcc ttccctttcg gggccgccca cgattcccag
      421 cgataggggg cgtcgagaat atatgcgtac ggcttcccgg acaagagggc ctcgtccgcc
      481 ctgtcctgtt ccagcccctc caggtatttc aaaaacagga gccacgaaga ctgctccgtg
      541 tagtcgagtt ccgtagtgca ccccgcctct tttctgagga ggtcgtcgat attccggaag
      601 gtctgctcga acatgcgttt gcctcttcct ttcatgtgtg aagaccgccg ggaatgttcc
      661 gccgatcggg tgtcatttat tccgtaatgt cattagcgtc acgatagcat tgatgagaag
      721 ggttgtcaat tttgctccgt caagaagcaa ggggcgagcc gatgactaga gggcgctgcc
      781 gatgcattaa aagagagcga ggcgcctctg ctgcggtgtg cgcgaccgtt ctatgagctg
      841 ctgctcttcg ggcgacgggg tcccgctcaa caggggagag gcgggatcat ggcgcgaggc
      901 attcgcgcgg acggccgccg cgctctgtgt ggcgtagcag tacgcgcacc cggcggggca
      961 ggtgtcgtac gcgccgatgt ccacgctcgg cgcgcaccgg cagtcggggc gctgcccggc
     1021 gtcctttttc tccggaacgg gcttccccga aagctccccg gcaagcgcgg catcgatgca
     1081 ccctcctggg aaacacccct ctgctccaaa gtccacggcc tcgcagcagg attgcaccgc
     1141 tatgccgtac gcttgggcaa ttttcgggaa accgcgagcg atggtccgca tatcttcccc
     1201 gccgacttcg tctatcccct tcccgcgcag cttccggt
//
LOCUS       DAOTIL010000934         1237 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminobacteriaceae bacterium isolate AMR_MDS_3026
            NODE_133557_length_1237_cov_3.301184, whole genome shotgun
            sequence.
ACCESSION   DAOTIL010000934 DAOTIL010000000
VERSION     DAOTIL010000934.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868050
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminobacteriaceae bacterium (wastewater metagenome)
  ORGANISM  Aminobacteriaceae bacterium
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae.
REFERENCE   1  (bases 1 to 1237)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1237)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:19:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,224
            CDSs (total)                      :: 3,198
            Genes (coding)                    :: 3,184
            CDSs (with protein)               :: 3,184
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1237
                     /organism="Aminobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_133557_length_1237_cov_3.301184"
                     /isolate="AMR_MDS_3026"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:3034609"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1153
                     /locus_tag="P5201_14615"
     CDS             <1..1153
                     /locus_tag="P5201_14615"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="necessary for biosynthesis of osmoregulated
                     periplasmic glucans possibly involved in the transfer to
                     the periplasmic space; Derived by automated computational
                     analysis using gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="glucan biosynthesis glucosyltransferase H"
                     /protein_id="HRV99829.1"
                     /translation="GIWLAYDIEGSYEETPPTLLDELKRDRRWCQGNLQHMRLIFTRG
                     FFPAHRALFLNGVLTYGSALLWLTFLIVNSAQAVSEALFEPIYMPILRKFLPSMPVWR
                     PHWALSLLGSTALLLFLPKVLSALPILVKDFRARAYGGPIRLYMSVFLEVVLSMILTP
                     IRMIFHSLFIVSTLLGKSIGWGTQSRDDRGTTWREAFAAHWWGTLLGIAWGGVVYWII
                     PSFFWWISPMIFSFVLSIPISVFTGRASVGRFFRKIGLFLVPSEVKLPPILQDLEDNL
                     ERPARYSPFGHSRKQGFLRAAVAPRTHALHIAMQLPRRKKKPSPLREEARKRIVEKAL
                     QLGPDALSAKEKTALLKDTDRLRELHRRVWTLEDEEKAALWGLALPKEE"
BASE COUNT          210 a          416 c          376 g          235 t
ORIGIN      
        1 cggcatctgg ctcgcctacg acatcgaggg gagctacgaa gagacgccgc cgacgcttct
       61 cgacgagctg aagcgcgacc gacgctggtg ccagggcaat ttgcagcaca tgcggctgat
      121 cttcactcgg ggtttcttcc ccgcgcaccg cgcgctcttc ctgaacggcg tcctcaccta
      181 cggctccgcg ctgctctggc ttacgttcct catcgtcaac tcggcgcagg ccgtctccga
      241 ggcgctcttc gagccgatct acatgcccat tctgcgcaag ttcctgccga gcatgcccgt
      301 ctggaggccc cactgggcgc tctccctgct cggctcgacc gcgctcctcc tctttttgcc
      361 aaaggtcctc tccgccctgc cgatcctcgt caaggacttc cgcgcgcggg catacggggg
      421 gccgatacga ctctacatga gcgttttcct cgaagtggtg ctctccatga tcctcacgcc
      481 catccggatg atcttccaca gcctcttcat cgtctcgacg ctcctcggaa agagcatcgg
      541 gtgggggacg cagtcgcggg acgaccgggg aacgacgtgg cgcgaggcgt tcgccgcaca
      601 ctggtggggg acgctcctcg gcatcgcgtg ggggggtgtc gtctactgga tcatcccgtc
      661 cttcttctgg tggatctcgc cgatgatctt ctccttcgtg ctctccatcc cgatctccgt
      721 cttcacgggg agagcctcgg tcggcaggtt cttcagaaag atcggcctct tcctcgtccc
      781 gtcggaggtc aagctgccgc ccatcctcca ggacctggag gacaacctgg agcgccccgc
      841 gcgctactcc ccgttcgggc attcgcggaa gcagggcttt ctgcgcgccg ccgtggcccc
      901 gcgcacccac gctctccaca tcgcgatgca gctcccccgc aggaagaaga aaccatcccc
      961 tctgcgggaa gaggccagaa agcgcatcgt ggagaaggcg ctccagctcg ggccggatgc
     1021 tctctcggcc aaggagaaga cggcgctctt gaaggacacg gatcgtcttc gcgaactgca
     1081 ccgccgcgtc tggactctgg aggacgagga gaaagccgcg ctctgggggc tcgcgctgcc
     1141 gaaggaagaa taggggatac gggcagggga atctttccgg aattgaagag aaggagggtc
     1201 agcgatgcac ggtacgaaaa aggagacaat ctgctac
//
LOCUS       DAOTIL010000935         1233 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminobacteriaceae bacterium isolate AMR_MDS_3026
            NODE_134304_length_1233_cov_2.058574, whole genome shotgun
            sequence.
ACCESSION   DAOTIL010000935 DAOTIL010000000
VERSION     DAOTIL010000935.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868050
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminobacteriaceae bacterium (wastewater metagenome)
  ORGANISM  Aminobacteriaceae bacterium
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae.
REFERENCE   1  (bases 1 to 1233)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1233)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:19:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,224
            CDSs (total)                      :: 3,198
            Genes (coding)                    :: 3,184
            CDSs (with protein)               :: 3,184
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1233
                     /organism="Aminobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_134304_length_1233_cov_2.058574"
                     /isolate="AMR_MDS_3026"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:3034609"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(89..>1233)
                     /locus_tag="P5201_14620"
     CDS             complement(89..>1233)
                     /locus_tag="P5201_14620"
                     /inference="COORDINATES: protein motif:HMM:TIGR01910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ArgE/DapE family deacylase"
                     /protein_id="HRV99830.1"
                     /translation="LGEKMRSLGCEVEYVEVPEEALASLPEESKGFPRYIVVGTYKGT
                     RERPAIHFSGHYDVVPAGGGWTVDPYGGLIADGKIFGRGASDMKSGIAAQIFALETLQ
                     HAGIRLKGSFVSSAVPDEETGGQPGMGYLVRSGRLTKENTDYCIITEPLDPDMICLGH
                     RGTLWFTLAFKGVQSHGSMPSEGLNPIEGMRALLERIDRRIRPQLERVSKYPVSPERS
                     RKSTLCITTIQAGSKVNTIPDTCSASFDWRLIPEQSVEWAKGEILSICDDLVREGVIL
                     EYAYTPIIEADPTMVDEDQPLVDALKTCGKAVLGKEMGINFSPGMDDQRFAVHEGGLG
                     AAVVYGPGRLALAHKSDEHIAVDDLVKGVEIMAAVAAELLGTEERQ"
BASE COUNT          194 a          413 c          385 g          241 t
ORIGIN      
        1 gtatcgtttc attccgccgc gcggcggacg tcagaagacg cgccggcggc gaatgaacgc
       61 caaaaacttt gccgtgctca cgatccggct actgtcgctc ctccgtcccg agcagctccg
      121 ccgcgaccgc cgccatgatt tcaacgccct tgacgagatc gtcgacggcg atatgctcgt
      181 ctgatttatg agcgagcgca agacgcccgg gcccgtacac cacagccgcc ccgaggccgc
      241 cttcgtggac ggcgaagcgc tgatcgtcca ttccggggct gaagttgatc cccatttcct
      301 tccccaggac ggccttcccg caggtcttca gcgcgtcgac cagcggctgg tcttcgtcga
      361 ccatggtggg gtcggcttcg atgatcggag tataggcgta ctcgaggatg acgccctcgc
      421 gcacaagatc gtcgcagatg gagagaatct cccccttcgc ccactccacg ctctgctcgg
      481 ggatgaggcg ccagtcgaag ctcgcggagc aggtgtcggg aatcgtattg accttcgatc
      541 cggcctggat ggtcgtgatg cagagggtgc tcttccggga gcgttcgggg ctgacggggt
      601 atttgctgac gcgctccagc tgcggacgga tgcgccggtc gatgcgttcg agcagcgccc
      661 tcataccctc gatggggttc agcccctccg acggcatgct cccgtggctc tgcactccct
      721 tgaaggcaag ggtgaaccag agggttccgc ggtgccccag gcagatcatg tcggggtcga
      781 gcggctcggt gatgatgcag tagtcggtgt tctccttcgt cagcctgccg ctccggacga
      841 ggtagcccat gccgggttgt cctccggtct cctcgtcggg gaccgcgctg gagacgaagc
      901 tccccttcag ccgaatgcct gcgtgctgga gcgtttcgag ggcgaagatc tgcgcggcga
      961 tgccggactt catgtcgctc gcgccgcgcc cgaaaatttt cccgtcggcg atcaggcctc
     1021 cgtacgggtc gaccgtccag ccgcctccgg ccgggacgac gtcgtagtgc cccgaaaagt
     1081 ggatcgccgg acgctccctc gtccctttgt acgtgccgac cacgatgtat ctcgggaagc
     1141 ctttcgactc ctccgggagc gacgcgagcg cctcctccgg cacttcgacg tactccactt
     1201 cgcatccgag cgaccgcatc ttctcgccaa gaa
//
LOCUS       DAOTIL010000936         1231 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminobacteriaceae bacterium isolate AMR_MDS_3026
            NODE_134568_length_1231_cov_4.259354, whole genome shotgun
            sequence.
ACCESSION   DAOTIL010000936 DAOTIL010000000
VERSION     DAOTIL010000936.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868050
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminobacteriaceae bacterium (wastewater metagenome)
  ORGANISM  Aminobacteriaceae bacterium
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae.
REFERENCE   1  (bases 1 to 1231)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1231)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:19:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,224
            CDSs (total)                      :: 3,198
            Genes (coding)                    :: 3,184
            CDSs (with protein)               :: 3,184
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1231
                     /organism="Aminobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_134568_length_1231_cov_4.259354"
                     /isolate="AMR_MDS_3026"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:3034609"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..171)
                     /locus_tag="P5201_14625"
     CDS             complement(<1..171)
                     /locus_tag="P5201_14625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016289993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HRV99831.1"
                     /translation="MRNVSKAFPGVQALRDVTLQVRPGTVHALMGENGAGKSTLMKCL
                     FGLYSLDEGEVIL"
     gene            complement(319..>1231)
                     /locus_tag="P5201_14630"
     CDS             complement(319..>1231)
                     /locus_tag="P5201_14630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015556025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="galactose ABC transporter substrate-binding
                     protein"
                     /protein_id="HRV99832.1"
                     /translation="AIYKFDDTFMTGVRNAIAEAAKGKAKVDIVDSQNSQSTQNDRVD
                     MFITKRMNALAINPVDRTAAGVIIDKAKAENIPVVFFNREPLPEDMQKWDKVYYVGAR
                     AEESGTMSGQIIVDFWKSHPEADKNGDGVLQYVMLKGEPGHQDAELRTEFSIKAVTDA
                     GIKVEKLAEDTGLWDRVRGQEKMAAFLAAHGDKIEAVFGNNDDMALGAIEALKAAGYF
                     SDGKFMPVVGVDATAPAIQALEDKTLLGTVLNDAVNQGVATFNLSYILAKGETPTEEN
                     SGYVISDGKYVWVPYRKVTLENVAEFK"
BASE COUNT          216 a          392 c          334 g          289 t
ORIGIN      
        1 caggatcacc tccccttcgt caagagaata caggccgaag aggcacttca taagcgtcga
       61 ctttcctgcg ccattctctc ccatcagcgc atgaacggtc cccgggcgca cttgcagcgt
      121 gacgtcgcga agcgcctgca cccccggaaa tgcctttgaa acattccgca tctcaagtgc
      181 atacttgtcg tttttttcca cgaagacacc ttcccttcgc atgcctctat cgctcccgga
      241 gcacaatgcc agggaacgat agcaaagaaa gggaggcgct tgcccccctt tcaaagctgc
      301 ctttccgcaa acgacgttct atttgaactc cgcgacgttc tccagagtga cctttctgta
      361 gggaacccag acgtacttcc cgtcgctgat gacgtatccg ctgttctcct cggtcggggt
      421 ctcgcccttc gccaaaatgt aggaaagatt gaaggtcgcg actccctggt tgactgcgtc
      481 gttcagcact gtgccgagga gcgtcttgtc ctcaagggcc tgaatcgccg gcgcggtcgc
      541 gtcgacgcca acaacgggca taaactttcc gtcgctgaag tagcctgccg ctttcagggc
      601 ttcgatcgcc ccaagcgcca tgtcgtcgtt attgccgaag accgcttcga tcttgtcgcc
      661 gtgcgccgcg aggaaggccg ccatcttctc ctgaccgcgg accctgtccc acagccccgt
      721 atcctccgcg agcttctcga ccttgattcc cgcgtcggtt accgccttga tggagaactc
      781 ggtacggagc tcggcatcct gatgccccgg ctctcccttg agcatgacgt actgaaggac
      841 gccgtcgccg tttttgtccg cttcggggtg gctcttccag aagtcgacga tgatctgccc
      901 cgacattgtg ccggactctt ccgcgcgcgc ccccacgtag tagaccttgt cccacttctg
      961 catatcctcg ggaagaggtt cgcggttgaa gaagactacg gggatgttct cggccttggc
     1021 cttgtcgatg ataacgccgg ccgcggtacg atccaccggg ttgatggcga gcgcattcat
     1081 cctcttggtg atgaacatgt cgacccggtc gttctgggtg gactgggagt tctggctgtc
     1141 gacgatatcg actttcgcct tgcccttggc cgcctcggcg atcgcgtttc tgacgccggt
     1201 catgaaggtg tcgtcgaatt tgtagatggc g
//
LOCUS       DAOTIL010000937         1231 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminobacteriaceae bacterium isolate AMR_MDS_3026
            NODE_134629_length_1231_cov_2.655612, whole genome shotgun
            sequence.
ACCESSION   DAOTIL010000937 DAOTIL010000000
VERSION     DAOTIL010000937.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868050
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminobacteriaceae bacterium (wastewater metagenome)
  ORGANISM  Aminobacteriaceae bacterium
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae.
REFERENCE   1  (bases 1 to 1231)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1231)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:19:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,224
            CDSs (total)                      :: 3,198
            Genes (coding)                    :: 3,184
            CDSs (with protein)               :: 3,184
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1231
                     /organism="Aminobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_134629_length_1231_cov_2.655612"
                     /isolate="AMR_MDS_3026"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:3034609"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>1231
                     /locus_tag="P5201_14635"
     CDS             <1..>1231
                     /locus_tag="P5201_14635"
                     /inference="COORDINATES: protein motif:HMM:NF013736.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase dimerization/phospho-acceptor
                     domain-containing protein"
                     /protein_id="HRV99833.1"
                     /translation="AALEESERSKSVLIANLPGIAYRCRYDEEWTMEFVSEGCLRLTG
                     YASDDLLFNKKISFNKLMLPEYREYARAVWERAMAERVPAALEYRILTAEGEEKWVFE
                     QGVPLFDETGTVEFLEGLIIDISSRKALEDRLQSTLGLQGLLAEISAFFINATLANID
                     EKIDAMLERCGEFLKVDRTFLFQLSEDCTLMSNTHEWSSPGIPAVKDDVQNYPVADVP
                     LIEKTICAQEMLFIPDVSELPEEWSAEKRELERQQVRSVLCLPLIKGGRFLGYFGFDA
                     VRATKQLDDETVEVLKILANTLADALESNRIEKEMLEAKTRAEDATRAKSEFLANMSH
                     DIRTPMNGVLGMTALLLDTQLSEEQRRFAETVRSSAESLLGILNDILDFSKIEAGKLD
                     LENLDFNLSALLDDLLAG"
BASE COUNT          274 a          349 c          370 g          238 t
ORIGIN      
        1 gccgcgcttg aagagagcga acgaagcaaa tcagtgctga tagccaacct gccggggatt
       61 gcctatcgtt gccgctacga cgaagagtgg acaatggagt tcgtctccga agggtgtctc
      121 cgtttgaccg ggtacgccag cgacgatctg ctgttcaaca aaaaaatctc gttcaacaag
      181 ctgatgctgc cggagtacag ggagtacgcc cgcgccgtct gggaacgagc gatggcggag
      241 cgcgttccgg cggcgctgga gtacagaatc ctgacggcgg agggggagga gaagtgggtc
      301 ttcgaacagg gcgttcccct ctttgacgag acgggaacgg tcgagtttct tgaaggactc
      361 atcatcgaca tctcttcgcg gaaagctctt gaggatcgac ttcaaagcac tcttggactc
      421 caagggctgc tcgcggagat ttccgccttt ttcatcaatg ccacgctcgc caacatcgac
      481 gagaagatcg acgccatgct cgaacgttgc ggcgagtttc tcaaggtgga caggaccttc
      541 ctcttccagc tttcggagga ctgtacgctc atgtccaaca cgcacgagtg gagctctccc
      601 ggaatacctg cggtgaagga cgacgtgcag aactaccccg tcgccgacgt cccgctgatc
      661 gagaaaacaa tctgcgcaca ggagatgctc tttatcccgg acgtctcgga gctgccggag
      721 gagtggagcg cggagaagcg cgagctcgaa cgacagcagg tgcgttctgt gctctgtctg
      781 ccgctgatca aaggcggccg tttcctcgga tatttcggtt tcgacgcggt ccgcgcaacg
      841 aagcagctcg acgacgagac ggtcgaagtg ctcaagatcc ttgcgaatac attggccgac
      901 gctctggaga gcaacaggat cgaaaaagag atgctggagg ccaaaacccg cgccgaggac
      961 gcgacccgcg cgaagagcga attcctcgcc aacatgagcc acgacatccg cacgccgatg
     1021 aacggggtgc tcggcatgac ggcgcttctg ctggacacac agttgagcga agagcagcgc
     1081 cggttcgccg aaaccgtccg ttcgagcgcg gagtcgctcc ttggcattct caacgatatt
     1141 cttgatttct ccaagatcga agccggaaag ctcgatctgg agaaccttga tttcaacctc
     1201 tccgctctcc tggacgatct tctggccggg t
//
LOCUS       DAOTIL010000938         1231 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminobacteriaceae bacterium isolate AMR_MDS_3026
            NODE_134630_length_1231_cov_2.647109, whole genome shotgun
            sequence.
ACCESSION   DAOTIL010000938 DAOTIL010000000
VERSION     DAOTIL010000938.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868050
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminobacteriaceae bacterium (wastewater metagenome)
  ORGANISM  Aminobacteriaceae bacterium
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae.
REFERENCE   1  (bases 1 to 1231)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1231)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:19:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,224
            CDSs (total)                      :: 3,198
            Genes (coding)                    :: 3,184
            CDSs (with protein)               :: 3,184
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1231
                     /organism="Aminobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_134630_length_1231_cov_2.647109"
                     /isolate="AMR_MDS_3026"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:3034609"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..716)
                     /locus_tag="P5201_14640"
     CDS             complement(<1..716)
                     /locus_tag="P5201_14640"
                     /inference="COORDINATES: protein motif:HMM:NF022743.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3100 domain-containing protein"
                     /protein_id="HRV99834.1"
                     /translation="MNKALLDPKIHITVLLVTVLSEFIGIKRVTVGPGVMLFLPMLYA
                     LVITGALTIKRLGFFKREQSVAASPLITLSILFLVARIAVIIGPNIMTIIAAGPALLL
                     QELGNLGTIFLALPVALLLGLKREAVGATHSIAREPNVALISEDYTLDSPEGLGVLGV
                     YVIGTLFGAIFMGLFAGFLASTTSLHPYALAMASGVGSGSMMAASSGSLLALFPDMKD
                     QIAAFAGASNLISTGSGLYVS"
     gene            complement(722..>1231)
                     /locus_tag="P5201_14645"
     CDS             complement(722..>1231)
                     /locus_tag="P5201_14645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014806917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HRV99835.1"
                     /translation="IDPQQPAVVSLGTIHAGTANNVIPDSVEMTGTVRTFDADLRASM
                     EGMLRRVVENTAAGFGCKGELFYRYDLPPVMNAPDATEIGRRAVAAVAGTDGVVVPVP
                     SMGGEDFALLQEKAPGCFFWLGVGNPEIGAVHPWHSPLFTADESALSIGAGVLAQSAL
                     FALQFLRGE"
BASE COUNT          249 a          335 c          432 g          215 t
ORIGIN      
        1 gagacgtaga ggccgctccc ggtggagatg agattggacg cgccggcgaa ggccgcgatc
       61 tgatctttca tatcggggaa gagcgcgagg agactgcccg aggaggccgc catcatgctg
      121 ccgctgccga cgccggacgc cattgcgagg gcgtaggggt gcagcgatgt cgtagaggca
      181 aggaagcctg cgaagaggcc catgaagatg gcgccgaaga gcgttccgat gacgtagacg
      241 cccagtacgc ccagtccttc gggagaatcg agggtgtagt cttcgctgat gagggcgacg
      301 ttcggttcgc gggcgatgga gtgggtcgcg ccgacggcct cccgcttcag ccccagcaga
      361 agagcgacgg gaagcgcgag gaagatggtg cccaagttgc cgagttcctg caaaagcagc
      421 gcgggaccgg cggcgatgat ggtcatgatg ttggggccga tgatgaccgc gatccgtgcg
      481 acgaggaaaa gaatcgagag cgtgatgaga ggggaggcgg cgacgctctg ttctctcttg
      541 aagaagccga ggcgctttat cgtgagcgct cccgtgatga cgagggcgta gagcatgggt
      601 aaaaagagca tcacgccggg gccgacagtg acgcgtttaa ttccgatgaa ttcgctgagc
      661 accgtgacga gaaggacggt gatatggatt ttcggatcga gcagggcttt gttcattgta
      721 ctcactctcc tcttaagaat tgcagcgcaa aaagcgccga ctgggccagg acgccggctc
      781 cgattgagag ggccgactcg tcggcggtaa acaaggggct gtgccagggg tggacggccc
      841 ctatctccgg attgcctacg ccgagccaga agaagcatcc gggggccttc tcctgcaaaa
      901 gggcgaagtc ctcgcctccc atcgagggga cggggacgac gacgccgtcc gttcccgcga
      961 ctgccgcgac tgcccggcgg cctatctccg tagcgtccgg ggcgttcatt acgggaggta
     1021 gatcgtagcg ataaaaaagc tctcccttgc aaccgaagcc tgcggcggta ttctcgacga
     1081 ctctgcggag catgccttcc atcgaggcgc ggagatccgc gtcgaaggtc cggacggtgc
     1141 cggtcatttc cacgctgtcg gggatgacgt tgttcgccgt tcccgcatgg attgtcccga
     1201 gcgaaacgac cgccggctgt tgaggatcga t
//
LOCUS       DAOTIL010000939         1230 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminobacteriaceae bacterium isolate AMR_MDS_3026
            NODE_134780_length_1230_cov_3.028936, whole genome shotgun
            sequence.
ACCESSION   DAOTIL010000939 DAOTIL010000000
VERSION     DAOTIL010000939.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868050
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminobacteriaceae bacterium (wastewater metagenome)
  ORGANISM  Aminobacteriaceae bacterium
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae.
REFERENCE   1  (bases 1 to 1230)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1230)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:19:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,224
            CDSs (total)                      :: 3,198
            Genes (coding)                    :: 3,184
            CDSs (with protein)               :: 3,184
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1230
                     /organism="Aminobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_134780_length_1230_cov_3.028936"
                     /isolate="AMR_MDS_3026"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:3034609"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            3..>1230
                     /gene="dnaK"
                     /locus_tag="P5201_14650"
     CDS             3..>1230
                     /gene="dnaK"
                     /locus_tag="P5201_14650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016292151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaK"
                     /protein_id="HRV99836.1"
                     /translation="MAVIEGGTPEVIANAEGGRTTPSVVAFTKDGERLVGSLAKRQAT
                     LNPEGTLFSVKRFIGRKYNEVASELKIVPYKVNEGANGAVRFSVGGKEYAPEEISASV
                     LRKLVSDASSYLGEKITDVVITVPAYFNDAQRQATKDAGTIAGLNVLRVVNEPTAAAL
                     AYGLDKKKEQKIMVFDLGGGTFDVSILDIGDGVVEVLSTSGDTHLGGDDFDKVVVDWI
                     ADEFRKENGIDLRKDKQALQRLYEAAEKAKCELSSRTSTEISLPFITADANGPRHLNM
                     AITRAKFDQLTAPLVERCAGPVKDALSAAKLSEKDIDEVVLVGGSTRIPAVQDVVRKL
                     TGGKQPNMSVNPDEVVAVGAAVQAGIISGEMKDVLLLDVIPLSLGVETMGGVMTKLIE
                     KNTTIPCRKSEIFSTAA"
BASE COUNT          286 a          341 c          382 g          221 t
ORIGIN      
        1 tcatcgcagt tatagaaggt ggtactccgg aagtcatcgc gaacgcggag ggagggcgaa
       61 caactccttc ggttgtcgct tttacaaaag acggcgagcg tctggtggga tcgcttgcaa
      121 aacggcaggc gacattgaac ccggaaggaa cgctcttttc tgtaaagcga tttataggac
      181 ggaaatacaa cgaggtggct tccgagctga agattgttcc gtacaaggtg aacgaagggg
      241 ccaacggagc cgtcaggttt tcggttggag gaaaagagta cgcgcccgag gagataagcg
      301 cctccgtgct ccgcaagctg gtcagcgacg cgtcgtccta tctcggcgag aagataacgg
      361 acgtcgtgat cacggttccg gcctacttta acgacgcgca gcgccaagcc accaaggatg
      421 cggggaccat cgccgggctg aacgtgctcc gcgtggtgaa cgaaccgacg gcggcggctc
      481 ttgcctacgg actcgacaag aaaaaagaac agaaaatcat ggtcttcgac ctcggaggag
      541 gcacgttcga cgtctctatt ctcgacatcg gcgacggggt agtggaagtg ctttctactt
      601 ccggcgacac ccacctcgga ggcgacgact tcgacaaggt cgtcgtcgac tggatcgccg
      661 acgagttccg caaggagaac ggcatcgacc tccgcaagga caagcaggcg ctccagcggc
      721 tctacgaagc ggcggagaag gcgaaatgcg agctctcgtc gaggacgagc acggagatca
      781 gtctcccctt catcaccgcc gacgcaaacg gccccagaca tctgaacatg gcgattacgc
      841 gggccaagtt cgaccaactt actgctcccc tcgtagagcg ctgcgccggc cccgtaaaag
      901 acgcgctctc cgcggccaag ctctccgaga aggatatcga cgaagtggtc ctcgtaggag
      961 gttccacgcg cattccggcg gttcaggatg ttgtccgcaa actcacgggt ggtaaacagc
     1021 ccaatatgtc ggtgaacccg gacgaagtgg tggcggtagg cgcggcggta caggccggca
     1081 ttatcagcgg cgagatgaag gatgtgctcc tcctcgacgt tattccgctc tctctcggcg
     1141 tggagaccat gggcggcgtg atgacgaagc tcatcgagaa aaataccacg attccgtgca
     1201 ggaaatctga gattttctcg actgcggcgg
//
LOCUS       DAOTIL010000940         1226 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminobacteriaceae bacterium isolate AMR_MDS_3026
            NODE_135392_length_1226_cov_3.907771, whole genome shotgun
            sequence.
ACCESSION   DAOTIL010000940 DAOTIL010000000
VERSION     DAOTIL010000940.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868050
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminobacteriaceae bacterium (wastewater metagenome)
  ORGANISM  Aminobacteriaceae bacterium
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae.
REFERENCE   1  (bases 1 to 1226)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1226)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:19:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,224
            CDSs (total)                      :: 3,198
            Genes (coding)                    :: 3,184
            CDSs (with protein)               :: 3,184
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1226
                     /organism="Aminobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_135392_length_1226_cov_3.907771"
                     /isolate="AMR_MDS_3026"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:3034609"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..504)
                     /locus_tag="P5201_14655"
     CDS             complement(<1..504)
                     /locus_tag="P5201_14655"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRV99837.1"
                     /translation="MNPVIQLNVLKCILSIRRTKTEKTNIFFYEILSPAGLFASGIAF
                     SPDDDEKSKKINVEVGAEAFLSFTEELSTRQEAFGKLLSPSVLSSLERLLGEILTAEA
                     VREALENPRDNMKTLEECLREEISSRLGYRATAYLKADRRGEEALAAFLKKDGAVLSS
                     EDANKESA"
     gene            complement(664..1191)
                     /gene="thiW"
                     /locus_tag="P5201_14660"
     CDS             complement(664..1191)
                     /gene="thiW"
                     /locus_tag="P5201_14660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005661034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy coupling factor transporter S component
                     ThiW"
                     /protein_id="HRV99838.1"
                     /translation="MRFWFQTDKTGDRSRVEKLTLAGLFVAAAILLSGVHFPMGPTRC
                     YPFQHTVNVLAGAFLGPWWAAGVAFITSIIRNMTGMGTLFAFPGSIPGAIAAGLAFKF
                     FSRPVSALAEPLGTGPVGAALSALLLGPAMGKPVGFLTLQTAFLISSIPGALLGMGIL
                     HIFQKAPFSRVLRGR"
BASE COUNT          292 a          334 c          319 g          281 t
ORIGIN      
        1 cgccgattct ttgtttgcat cttcggagga aagaacggcg ccgtcttttt taaggaacgc
       61 agcgagcgcc tcctctcccc tgcgatccgc cttgagatac gcagttgctc gatacccgag
      121 ccgggaagaa atctcttccc ggaggcactc ttccagggtt ttcatattat cgcgcggatt
      181 ttccaacgcc tctcttactg cctctgcggt gagtatttcg cctaagaggc gctccagaga
      241 ggagaggaca gagggagaga gcagcttccc gaaagcttct tgacgagtgg agagctcttc
      301 ggtgaagctg agaaatgcct cggctcccac ttcgacgtta atttttttac tcttttcgtc
      361 atcgtccggt gaaaacgcta taccggaggc gaacagtccc gcaggggata aaatctcgta
      421 gaaaaagatg tttgtctttt ccgtttttgt cctccgtatt gagaggatgc acttcaaaac
      481 gttcaactgt attacaggat tcacaggttt tctccctttc ggtattgatt ccgagaatgg
      541 cattatacgt tgttttccct gttcgcgaaa gccccggacg ccaaataggc tggaatatgc
      601 attcgaaaac tacggagcgg gttcgtccct gagaagaacg gaaggactcg ctccgtagtt
      661 ttcttaccgc cctcggagta ccctgctgaa aggcgccttc tgaaagatgt gaagaatacc
      721 cattccgaga agcgccccgg gtatgctgct gataagaaat gcagtctgta acgtaagaaa
      781 gccgacaggc tttcccatgg cgggaccaag cagaagcgcc gaaagcgcag ctccaacagg
      841 accggtgccg agcggttcgg caagcgcgct tacaggccgg gagaaaaact tgaaagccaa
      901 acccgcagcg atcgcgcccg ggatgcttcc gggaaacgcg aacagcgtcc ccatgcccgt
      961 catatttcgg atgatgctgg tgataaaggc gactcccgca gcccaccatg gaccgagaaa
     1021 cgcccccgca agcacgttga ccgtatgttg aaacggatag catcgcgtcg gccccatagg
     1081 gaaatgaact cctgaaagaa gaatcgccgc cgccacaaaa agacctgcga gggtcagttt
     1141 ttcgacccgg cttctgtctc cggttttatc tgtctgaaac cagaagcgca ttcagatcca
     1201 ccttcttttc tatcattccg caggtg
//
LOCUS       DAOTIL010000941         1226 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminobacteriaceae bacterium isolate AMR_MDS_3026
            NODE_135419_length_1226_cov_3.028181, whole genome shotgun
            sequence.
ACCESSION   DAOTIL010000941 DAOTIL010000000
VERSION     DAOTIL010000941.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868050
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminobacteriaceae bacterium (wastewater metagenome)
  ORGANISM  Aminobacteriaceae bacterium
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae.
REFERENCE   1  (bases 1 to 1226)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1226)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:19:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,224
            CDSs (total)                      :: 3,198
            Genes (coding)                    :: 3,184
            CDSs (with protein)               :: 3,184
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1226
                     /organism="Aminobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_135419_length_1226_cov_3.028181"
                     /isolate="AMR_MDS_3026"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:3034609"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..951
                     /gene="ptsP"
                     /locus_tag="P5201_14665"
     CDS             <1..951
                     /gene="ptsP"
                     /locus_tag="P5201_14665"
                     /EC_number="2.7.3.9"
                     /inference="COORDINATES: protein motif:HMM:NF014895.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008965 - phosphoenolpyruvate-protein
                     phosphotransferase activity [Evidence IEA];
                     GO_process: GO:0006808 - regulation of nitrogen
                     utilization [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate--protein phosphotransferase"
                     /protein_id="HRV99839.1"
                     /translation="TVTLDGIKVELGANVGSSRDLDEVLKNDGEAVGLYRTEFLYLES
                     PMLPTEDEQFEAYKSVVEGMDGRPVVIRTLDIGGDKKLDYLSIPEEENPFLGYRAIRL
                     CLDRQEIFKVQLRAILRSSAYGNVRILFPMISCLEELREAKVILENTKDDLRQRKIPF
                     DEGVLAGIMVEVPSTAVLSDHFAKESDFFSIGTNDLIQYTVAVDRGNDKLAPLYSSYH
                     PAVLRLVKTVIDNGKQAGIHVGMCGESAGDPKLIPILIGMGLTEFSMSPPSILQARWI
                     LRNLRKSDLAEAARHALSLGTAAEVEAYCGTLLQSLDLCR"
     gene            912..>1226
                     /locus_tag="P5201_14670"
     CDS             912..>1226
                     /locus_tag="P5201_14670"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRV99840.1"
                     /translation="MRHAASVARPLPLNGGPAARGPIAVMSASIRGTNHSGLPEFLRP
                     LFWDAEWSAVDFRRNRTAIIERVLNLGDEAQLAWLKQNVAPEEIRAVVLSSRRLSKKT
                     ARC"
BASE COUNT          228 a          392 c          392 g          214 t
ORIGIN      
        1 accgtcacac tcgacggaat caaggtcgag ctgggcgcga acgtcggttc ttcccgcgat
       61 ctcgacgagg tgctgaagaa cgatggcgag gccgtcggcc tctatcgaac ggagttcctc
      121 tacctcgaaa gcccgatgct ccccaccgag gacgagcagt ttgaagccta caagagcgtc
      181 gtcgagggga tggacggccg tccggtggtc atcaggacgc tcgacatcgg cggcgacaag
      241 aagctcgact acctctccat tccggaggag gagaacccgt tcctcggcta ccgcgccatc
      301 cgtctctgcc tggaccggca ggagatattc aaggtgcagc tccgggcgat cctccgatcg
      361 agcgcctacg gcaacgtgcg catcctcttc ccgatgatct cctgtcttga ggagctccgg
      421 gaagcgaagg tcatcctcga gaacacgaag gacgatctcc gtcagcggaa gattccgttc
      481 gacgagggcg tcctggcggg cattatggtg gaggttccct cgaccgccgt gctctccgac
      541 cacttcgcga aggagtccga cttcttcagc atcggcacga acgacctcat ccagtacacc
      601 gtggcggtcg accgcgggaa cgacaaactg gcgccgctct attcctccta ccacccggcg
      661 gtgctccggc tcgtgaaaac cgtgatcgac aacggcaagc aggccggtat ccacgtcggg
      721 atgtgcggcg agtcggcggg ggatcccaag ctgatcccga tcctgatcgg gatggggctg
      781 acggagttca gcatgagccc tccgtccatt ctgcaagccc ggtggattct gcggaacctt
      841 cggaagagcg acctggccga agcggcgcgg cacgctcttt cgctggggac ggctgcggag
      901 gtggaggcgt attgcggcac gctgcttcag tcgctcgacc tctgccgctg aacggaggac
      961 cggccgcgcg cggcccgatc gccgtcatga gcgcatcaat ccgcgggacg aatcattccg
     1021 gactccccga gtttctgcgc cccctcttct gggacgcgga gtggtccgcc gtggactttc
     1081 ggcgcaaccg cacggcgatc atcgaacggg tgctgaacct gggggacgag gcgcagcttg
     1141 cgtggctgaa acagaatgtc gcgcccgaag agatacgcgc cgtggtgctt tcgagccgcc
     1201 gtctgagcaa aaagaccgcc cgctgc
//
LOCUS       DAOTIL010000942         1224 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminobacteriaceae bacterium isolate AMR_MDS_3026
            NODE_135747_length_1224_cov_2.846878, whole genome shotgun
            sequence.
ACCESSION   DAOTIL010000942 DAOTIL010000000
VERSION     DAOTIL010000942.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868050
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminobacteriaceae bacterium (wastewater metagenome)
  ORGANISM  Aminobacteriaceae bacterium
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae.
REFERENCE   1  (bases 1 to 1224)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1224)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:19:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,224
            CDSs (total)                      :: 3,198
            Genes (coding)                    :: 3,184
            CDSs (with protein)               :: 3,184
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1224
                     /organism="Aminobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_135747_length_1224_cov_2.846878"
                     /isolate="AMR_MDS_3026"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:3034609"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..438)
                     /locus_tag="P5201_14675"
     CDS             complement(<1..438)
                     /locus_tag="P5201_14675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014807269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="indolepyruvate ferredoxin oxidoreductase"
                     /protein_id="HRV99841.1"
                     /translation="MKSLGDMLLNTGPFSEIVLGNTALARAMIESGTEVIASYPGSPT
                     PEIGAALMSVPKERRPYYFEFSTNEKVATEVACGASLNGRPSCVFFKSVGLNVAADSF
                     VQLSLLELNGGLVAILGDDPGANSSQNEQDNRHIARLAYIPVLE"
     gene            complement(456..638)
                     /locus_tag="P5201_14680"
     CDS             complement(456..638)
                     /locus_tag="P5201_14680"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRV99842.1"
                     /translation="MESGDPLDSDEFLPLKDESGKVTILVPKSMKFTDDKVLVEHFTY
                     RGKDVFICRTALLPDR"
     gene            complement(660..827)
                     /locus_tag="P5201_14685"
     CDS             complement(660..827)
                     /locus_tag="P5201_14685"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRV99843.1"
                     /translation="MKKCIVLLGIAALLMVFALPAVSQELHLALEQSALEKLSAKEGA
                     LYIEWGVYRNT"
BASE COUNT          235 a          373 c          373 g          243 t
ORIGIN      
        1 ttcgagcacg gggatgtagg cgaggcgggc gatgtggcgg ttgtcctgct cgttctgcga
       61 ggagttcgcg ccggggtcgt cgccgaggat ggcgaccagg ccgccgttca gctccagtag
      121 cgagagctgg acgaaggagt cggccgcgac gttgagaccg acgcttttga aaaagacgca
      181 cgaggggcgc ccgttgagcg acgcgccgca cgcgacctcg gtggccacct tctcgttggt
      241 ggagaactcg aagtagtagg gacggcgctc tttgggaacg ctcatgagcg cggccccgat
      301 ctccggcgtg ggggagccgg gataggaggc gataacctcc gtcccggact cgatcattgc
      361 gcgggccagc gcggtgttcc cgaggactat ttcactgaac ggcccggtat tcagcagcat
      421 gtcgccgaga cttttcattt gaggacctcc ttcgtttacc ggtcgggcag gagagccgtc
      481 ctgcagatga aaacatcctt tccgcgatag gtgaagtgct ccacaagcac tttgtcgtcg
      541 gtgaacttca tgctcttggg cacaagaatg gtcaccttgc cggattcgtc cttcagcgga
      601 aggaattcat cggagtcgag gggatcgccg ctctccacga gagcggatgg cttctcccct
      661 caggtgttgc ggtagacgcc ccattcgatg tagagcgctc cttctttcgc cgacaacttt
      721 tcgagcgcgc tctgctccag cgcaaggtga agctcctgcg aaaccgccgg cagagcgaac
      781 accatcagca acgccgcaat tcccagcaat acgatacatt ttttcatgaa cgaagatctc
      841 ctcctcttat aaaatgaaac agattctctc cgggaattgt atcacggact gcgtgttgag
      901 tgctacactt gtcgcgagcc gcgaaatgat cgcacgagga ggcgttcccc ctgagactcc
      961 acacctaccg caccccccga agccttgcag aaccgctgcg cgaagaggcc gcgcgcggcg
     1021 acacgatctt tctgacgccg tcgaacaggg accgtccgct gctgctggag atgctcttcc
     1081 ttccggactc gatttccttc ccggagcgga agccgaaggt gtggatatgg gacgacctct
     1141 accgggcgct cgccgaggcc cttggcgtga aaccccgcgt gcagatcgac cctcccgacc
     1201 actggctgct cgtccggcac atcg
//
LOCUS       DAOTIL010000943         1222 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminobacteriaceae bacterium isolate AMR_MDS_3026
            NODE_136166_length_1222_cov_1.455013, whole genome shotgun
            sequence.
ACCESSION   DAOTIL010000943 DAOTIL010000000
VERSION     DAOTIL010000943.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868050
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminobacteriaceae bacterium (wastewater metagenome)
  ORGANISM  Aminobacteriaceae bacterium
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae.
REFERENCE   1  (bases 1 to 1222)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1222)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:19:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,224
            CDSs (total)                      :: 3,198
            Genes (coding)                    :: 3,184
            CDSs (with protein)               :: 3,184
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1222
                     /organism="Aminobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_136166_length_1222_cov_1.455013"
                     /isolate="AMR_MDS_3026"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:3034609"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..265)
                     /locus_tag="P5201_14690"
     CDS             complement(<1..265)
                     /locus_tag="P5201_14690"
                     /inference="COORDINATES: protein motif:HMM:NF025469.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4277 domain-containing protein"
                     /protein_id="HRV99844.1"
                     /translation="MGNLRDDRIVGGVEFQNCGVVAAFTERLGIVDFLDRHLPEPRRK
                     GASFGVIAKGLIIQILHARFKRIGFGGLFFNNLPTNQLLGRGVL"
     gene            complement(410..757)
                     /locus_tag="P5201_14695"
     CDS             complement(410..757)
                     /locus_tag="P5201_14695"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF014505.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004521 - endoribonuclease activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system PemK/MazF family
                     toxin"
                     /protein_id="HRV99845.1"
                     /translation="MNSPRRGEIWLVDWSPSRGSEQAGSRPALVLQTDAANSNPRYPN
                     TIVCALSTKGRDIPFHIPLEPHPRTGLSSPSYCKCEQILTISKERLVRRLGTVDEEVM
                     KRIEEGVRLVLDC"
     gene            complement(750..977)
                     /locus_tag="P5201_14700"
     CDS             complement(750..977)
                     /locus_tag="P5201_14700"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRV99846.1"
                     /translation="MQKTVTVRISEEMHRECQRLSRRRKVSMNSFLVEGARKMIEEEK
                     RRLLYDAFSEAADEGDVEFASEAQREILNDE"
BASE COUNT          271 a          354 c          306 g          291 t
ORIGIN      
        1 gcagaactcc gcggccgaga agctggttcg tgggaaggtt gttgaaaaaa aggccgccga
       61 atccgatacg cttgaaccgg gcatgaagga tctggatgat cagccccttc gcgatcactc
      121 cgaacgacgc gccttttctg cgaggttccg gtagatgcct gtcgagaaag tcgacgatgc
      181 ctaaccgctc cgtgaatgcg gcgactacac cgcagttttg gaactccaca cccccgacga
      241 tacgatcatc gcgtagatta cccaaaattt ccgcccccat catgcatgag aataaaaccg
      301 cactgcgaaa agcgccttca gaaccggact tttttctgtc cgcatcgcgg gcgctcttga
      361 agatgccgga catgcgaata caaccgccga gcgtttttct ttcaatgcat cagcaatcaa
      421 gcacaaggcg cacgccttcc tctatccgct tcatgacttc ctcgtctacg gttccaagtc
      481 ttcggacaag ccgctccttg gagatagtca atatctgttc gcacttgcaa tacgacggag
      541 acgaaaggcc tgtacgggga tgcggttcaa gcggaatatg gaacgggatg tctcttccct
      601 tggtgctgag agcacataca atggtgttag ggtatctcgg attgctattc gcagcgtccg
      661 tctggagaac aagtgccggg cggcttccag cctgctccga tcctctcgac ggagaccaat
      721 ccacaagcca tatctccccg cgacgaggac tattcatcgt tgagaatctc cctctgcgct
      781 tcggaggcga attcaacatc accttcgtcc gctgcttcgc tgaaagcatc gtacagaagg
      841 cgccgtttct cctcttcgat catctttctc gcgccctcga cgagaaagga gttcatactg
      901 actttcctgc ggcgggagag ccgctgacac tctcgatgca tttcttccga tattcgcaca
      961 gtgacggtct tctgcatgaa gcatcctcct ttgcatgcat tttttacatc attctctgtg
     1021 cgttttatga tagcacaaat agagggaagg aggtgcggat caatgcaaaa actcggccgg
     1081 tctttctgtg tgttcccaag actcggagtc ccttcattct ctctcaagga gagagggcgg
     1141 ctttcgccgc cctcttgtct tccatttcta ctcgccgcag agccagccga agaagcggct
     1201 ccagaagagc gggtagttcg cc
//
LOCUS       DAOTIL010000944         1221 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminobacteriaceae bacterium isolate AMR_MDS_3026
            NODE_136206_length_1221_cov_3.880789, whole genome shotgun
            sequence.
ACCESSION   DAOTIL010000944 DAOTIL010000000
VERSION     DAOTIL010000944.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868050
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminobacteriaceae bacterium (wastewater metagenome)
  ORGANISM  Aminobacteriaceae bacterium
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae.
REFERENCE   1  (bases 1 to 1221)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1221)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:19:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,224
            CDSs (total)                      :: 3,198
            Genes (coding)                    :: 3,184
            CDSs (with protein)               :: 3,184
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1221
                     /organism="Aminobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_136206_length_1221_cov_3.880789"
                     /isolate="AMR_MDS_3026"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:3034609"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..438)
                     /locus_tag="P5201_14705"
     CDS             complement(<1..438)
                     /locus_tag="P5201_14705"
                     /inference="COORDINATES: protein motif:HMM:NF014612.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="precorrin-8X methylmutase"
                     /protein_id="HRV99847.1"
                     /translation="MDEQRTRRHGAEFKTENGEEPVRKRDRDLSPSEIEEKSFRILEG
                     LLGDFQAPAPEREVMKRVAHATTDVEWAKTFRFSPNAVQAGIEAVRRGAPVVTDVEMV
                     SAGVRRSAAEARRFRNAQDGNETLCFLNDPDVAEEAKRSGTTRA"
     regulatory      complement(640..755)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00174"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="cobalamin riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="adenosylcobalamin"
                     /db_xref="RFAM:RF00174"
     gene            930..>1221
                     /locus_tag="P5201_14710"
     CDS             930..>1221
                     /locus_tag="P5201_14710"
                     /inference="COORDINATES: protein motif:HMM:NF012610.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HRV99848.1"
                     /translation="MSLKETYKTKSGFVYELLRERIVAGEWPQGTVIVVASVARELGV
                     SAIPVREAMKQLQAEGLVELEPHRGARVTVFDPKKIVEVMSIRGALEGYAARV"
BASE COUNT          236 a          354 c          356 g          275 t
ORIGIN      
        1 cgcgcgggtc gttccgctcc gtttcgcctc ctcggcgacg tcgggatcgt tcaggaagca
       61 gagcgtttca tttccgtctt gggcgttccg gaatcgccgc gcttccgctg cggaacggcg
      121 aacgcccgcg ctcaccatct cgacgtcggt cacgacggga gcgccgcgcc gtacggcctc
      181 gatgcccgcc tgcactgcgt tcggcgagaa gcggaacgtc ttcgcccatt cgacgtcggt
      241 cgtggcgtgg gcgactctct tcatcacctc gcgctccgga gcgggagctt gaaagtcgcc
      301 cagcagtccc tcaagaatcc gaaagctctt ttcttctatt tccgaggggg agagatcgcg
      361 gtcgcgcttc cggacaggtt cttccccgtt ttctgttttg aattccgcgc cgtgccgccg
      421 cgtcctctgt tcgtccatgg aatctcctcc attcatacgg atacgcggag cgcgcaccgc
      481 tgaagacaaa aataaggcga acccccgtgt gaggttcgcc ggcgccggtc atatatatgc
      541 gacgagtgca aaatccggaa aaacctgtcc gtttgccgca aaaaatacat gaaaagccga
      601 cccgaaccct cgcaggtgtc gcacagatgc gcttcttcag ggtcttctgg cttccttcat
      661 cctcgttcac cccttcccgg caaaaaccgg tggtcgatga actcgtcggg tacacagcgg
      721 cgggaccgcg ccggaatttc accgggcttc cccttgaaga cgagcgcgtt catatagtat
      781 tcgatgaaca tctccatcct aatcttccgg tgtcgacctg tcaatcggat ttcgtggaac
      841 gggtgcattt cttcgtttga ctaggtataa tatgcatcat atattgtgct tttaaaaagg
      901 gcttgatttt tcaaaatcag gaggtttcca tgtcgctcaa agagacgtat aagacaaagt
      961 cggggttcgt atacgaactg ctgcgggaga gaattgtcgc cggagagtgg ccccagggaa
     1021 cggtgatcgt cgtcgcttcg gtcgccagag agctcggagt ctcggcgatc ccggtgcggg
     1081 aggcgatgaa gcagcttcag gccgaaggac ttgtcgaact tgagccgcac cggggggcga
     1141 gagttactgt attcgatccg aagaagatcg tcgaggtcat gtcgattcgc ggcgcgctcg
     1201 aagggtatgc cgcccgggtc g
//
LOCUS       DAOTIL010000945         1216 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminobacteriaceae bacterium isolate AMR_MDS_3026
            NODE_137047_length_1216_cov_3.749354, whole genome shotgun
            sequence.
ACCESSION   DAOTIL010000945 DAOTIL010000000
VERSION     DAOTIL010000945.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868050
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminobacteriaceae bacterium (wastewater metagenome)
  ORGANISM  Aminobacteriaceae bacterium
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae.
REFERENCE   1  (bases 1 to 1216)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1216)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:19:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,224
            CDSs (total)                      :: 3,198
            Genes (coding)                    :: 3,184
            CDSs (with protein)               :: 3,184
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1216
                     /organism="Aminobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_137047_length_1216_cov_3.749354"
                     /isolate="AMR_MDS_3026"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:3034609"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1216)
                     /locus_tag="P5201_14715"
     CDS             complement(<1..>1216)
                     /locus_tag="P5201_14715"
                     /inference="COORDINATES: protein motif:HMM:NF014900.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="pyruvate formate lyase family protein"
                     /protein_id="HRV99849.1"
                     /translation="LTVGGRKRDGSDGTNELSYICLKATEGVKTHQPGLSVRISSDCP
                     DDFLMAVSKLVATGMGFPAIHNDQAGAQMLLQAGYEPEDARDWNNCGCVVPHFRKTGE
                     WTSAVNVNFGAAMEYALNEGKSRLTGEPMGLPERAPEEFVSFREVADAFLRQLANLVE
                     HAVVGTLTAQKLHMEMVPRPFLSTCVDGCMQKGKDLSRGGAVYNVGPVLTGIGLGVVA
                     NSLAAVKKLVFDEKVCTMKELCAALDADWEGYDELRERALAAPKYGNDDDEVDSLARE
                     ISDFYYRTTRSHRDVFGSPFNSAFMGISNYVPTGKVIGALPCGRKARAPLSEGVSPFA
                     GTDVSTPLAAMRSAAKVNHDVQTGGTLLNLRLNKEIVSTPRGLRNLGAMIRAYFSLGA
                     FHVQFNTISTGEL"
BASE COUNT          196 a          405 c          393 g          222 t
ORIGIN      
        1 agctccccgg tggagatggt gttgaactgg acgtggaagg cccccagcga gaagtaggcg
       61 cgtatcatcg ccccgaggtt gcgcaggcct ctcggcgtag agacaatctc cttgttcagc
      121 cgcaggttga gcagcgtccc gccggtctga acgtcgtggt tcaccttggc cgcggaacgc
      181 atggccgcca gcggtgtgga gacgtccgtc cccgcgaagg gggagacgcc ctcggagagc
      241 ggcgcccgcg ccttcctccc gcagggaagc gcgccgatca ctttcccggt ggggacgtag
      301 ttggagattc ccatgaaggc ggagttgaag ggcgagccga agacatccct atggcttctg
      361 gtcgtcctgt agtagaagtc ggagatctcc cgtgccagcg aatcgacctc gtcgtcgtcg
      421 ttgccgtact tcggcgccgc aagggcgcgc tcccgcagtt cgtcgtaccc ctcccagtcg
      481 gcgtcgagcg cggcgcacag ctccttcatg gtgcagacct tctcgtcgaa gaccagcttc
      541 ttcaccgcgg cgagggagtt ggcgaccacg ccgaggccga taccggtgag gacggggccg
      601 acgttgtaca ccgcgccccc cctcgagagg tctttcccct tctgcataca gccgtccacg
      661 caggtcgaga ggaagggcct ggggaccatc tccatatgca gcttctgcgc cgtcagggtg
      721 cccacgacgg cgtgctccac aaggttggca agctgccgga ggaaggcgtc cgcgacctcg
      781 cggaaggaga cgaactcctc gggagctctc tcgggaagcc ccatcggctc tccggtgagg
      841 cggctttttc cttcgttgag ggcgtactcc attgccgcgc cgaagttcac gttcaccgcc
      901 gaggtccact cccccgtctt acggaagtgc gggaccacgc agccgcagtt gttccagtcg
      961 cgggcgtcct ccggctcgta cccggcctgg agcagcatct gcgccccggc ctggtcgttg
     1021 tggatcgcgg ggaagcccat tcccgtggcg acgagcttcg agaccgccat caggaagtcg
     1081 tccgggcaat ccgaagaaat acgaacgctc aacccgggct ggtgcgtctt cactccctcc
     1141 gtggccttca gacagatgta ggagagctcg ttcgtcccgt cgcttccgtc ccgcttccgc
     1201 ccgccgacgg tgagat
//
LOCUS       DAOTIL010000946         1214 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminobacteriaceae bacterium isolate AMR_MDS_3026
            NODE_137411_length_1214_cov_3.857636, whole genome shotgun
            sequence.
ACCESSION   DAOTIL010000946 DAOTIL010000000
VERSION     DAOTIL010000946.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868050
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminobacteriaceae bacterium (wastewater metagenome)
  ORGANISM  Aminobacteriaceae bacterium
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae.
REFERENCE   1  (bases 1 to 1214)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1214)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:19:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,224
            CDSs (total)                      :: 3,198
            Genes (coding)                    :: 3,184
            CDSs (with protein)               :: 3,184
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1214
                     /organism="Aminobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_137411_length_1214_cov_3.857636"
                     /isolate="AMR_MDS_3026"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:3034609"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..225)
                     /locus_tag="P5201_14720"
     CDS             complement(<1..225)
                     /locus_tag="P5201_14720"
                     /EC_number="2.1.1.148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014162896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_function: GO:0050797 - thymidylate synthase (FAD)
                     activity [Evidence IEA];
                     GO_process: GO:0006231 - dTMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent thymidylate synthase"
                     /protein_id="HRV99850.1"
                     /translation="MRCGVLLLSFTPRPDAVVAAAAKLCYSDVSAADLLAGESSEKMR
                     GLLCHIRASGHFSPFEHASFTFAVDGLSRVT"
     gene            complement(279..512)
                     /gene="rpmE"
                     /locus_tag="P5201_14725"
     CDS             complement(279..512)
                     /gene="rpmE"
                     /locus_tag="P5201_14725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008521739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L31"
                     /protein_id="HRV99851.1"
                     /translation="MKKDIHPHYEKCTVNCACGNSFETRSTQKEIRVGVCSSCHPFFT
                     GKKGSRVLTEAGRLEKFQKKYEGINYGQKESAE"
     gene            complement(633..1157)
                     /locus_tag="P5201_14730"
     CDS             complement(633..1157)
                     /locus_tag="P5201_14730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013048120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HutP family protein"
                     /protein_id="HRV99852.1"
                     /translation="MELLQGMEEEREPVTLQMNGPSADSERSSVELNLKIRIDDENQA
                     GRAALLLASTTTSEAENIFKRELASLGWKSVATEVGGLAGDLPQKITRALVGASLNAG
                     VVEKKRNEMHALMHAALEALDGFLVVGMLEASVGAKIAIVRNNRWISVAVMGDTAYHA
                     VAHHERCGLGVMHI"
BASE COUNT          251 a          377 c          311 g          275 t
ORIGIN      
        1 cgttaccctg ctcaagccgt ccacggcaaa ggtgaaggag gcgtgctcga acggggagaa
       61 atgaccgctt gcgcggatat gacacaacag accgcgcatc ttctccgaag attcacccgc
      121 gagaaggtcc gcagccgaaa cgtcgctgta acagagtttt gccgcagcgg cgacaacagc
      181 atcaggccgg ggcgtgaagg agagaaggag gacgccgcag cgcatacagc ccacttcctt
      241 tccgaaaaag aggggaccga agccccctcg tcgttgtcct attccgcgct ttccttctgg
      301 ccgtagttga ttccctcgta cttcttctgg aatttctcga gtcttccggc ctccgtgagc
      361 acacggctgc ccttcttccc ggtgaagaag gggtggcagg aggaacaaac gccgacgcga
      421 atctccttct gcgtcgaacg ggtctcgaaa gaatttccgc atgcacagtt caccgtgcac
      481 ttctcgtagt gaggatgaat atcttttttc atctatgggc acctccgaaa ttatcgtaaa
      541 gccttgcata tatctccaaa cgaagagaaa ctttagcaca ggaaggcccc ggacgcaaga
      601 gcaaattttc ttccgggacc gattttctcc cttcagatat gcatgactcc gaggccgcag
      661 cgttcgtgat gcgcgacggc atggtaggcc gtatctccca tgacggcgac ggagatccag
      721 cgattgttcc ggacgatggc gatcttcgcg ccgacgctcg cttcgagcat gcctacgacg
      781 agaaaaccgt ccaacgcttc cagcgcggcg tgcatcagcg catgcatctc gttccttttt
      841 ttctccacca cccccgcgtt gagcgaagcc ccgacaagcg cccttgtgat cttctgagga
      901 aggtctccag caagtccccc gacttccgtc gccaccgatt tccaacccag ggaagccagc
      961 tctctcttga aaatgttctc cgcctcgctg gtcgtcgtcg atgcgaggag aagggcggct
     1021 ctccccgcct gattttcgtc gtcgatacgt atcttgagat tgagttcgac cgagcttctc
     1081 tccgaatcag cgctcggccc gttcatctgc agcgtaacgg gctctctctc ctcctccatc
     1141 ccttgcaaaa gctccatctc gacgggttcc tcctcaatgt cttcgtcctg gggattcttg
     1201 aagatattct tcca
//
LOCUS       DAOTIL010000947         1213 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminobacteriaceae bacterium isolate AMR_MDS_3026
            NODE_137630_length_1213_cov_2.823834, whole genome shotgun
            sequence.
ACCESSION   DAOTIL010000947 DAOTIL010000000
VERSION     DAOTIL010000947.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868050
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminobacteriaceae bacterium (wastewater metagenome)
  ORGANISM  Aminobacteriaceae bacterium
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae.
REFERENCE   1  (bases 1 to 1213)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1213)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:19:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,224
            CDSs (total)                      :: 3,198
            Genes (coding)                    :: 3,184
            CDSs (with protein)               :: 3,184
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1213
                     /organism="Aminobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_137630_length_1213_cov_2.823834"
                     /isolate="AMR_MDS_3026"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:3034609"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..280
                     /locus_tag="P5201_14735"
     CDS             <1..280
                     /locus_tag="P5201_14735"
                     /inference="COORDINATES: protein motif:HMM:NF013677.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - transposition, DNA-mediated
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HRV99853.1"
                     /translation="AEFKTKVVLEMLREEETLSQIAARHKIHHTVLQNWKKTVTEGMP
                     GLFADPRKKSAEDLQKDSTMNDLYKQVGLLSMQLEWLKKKCGVDSFSS"
     gene            258..1124
                     /locus_tag="P5201_14740"
     CDS             258..1124
                     /locus_tag="P5201_14740"
                     /inference="COORDINATES: protein motif:HMM:NF033516.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HRV99854.1"
                     /translation="MSTLSLHERRALVDWENKDFPLSWQAWLLGLNRTGLYYVPGPLS
                     EEVLLLRRRIDEIHTDQPCYGSRRVQAVLSRGGLPVSRKRIQRLMRDMGILAIYPGPK
                     LSAGDESPKFPYLLRGVDIDHPNHTWGTDVTYVRLKGGFMYLTVFLDWYSRYVVSWAF
                     SDSLAAGFVVEALDEGLKVARPRIINSDQGSQYTSNLYIERIRAESESISISMDGRGR
                     CMDNIFTERFWRSYKQEEVYLKEYESPREARRSTAEYMKHYNFNRPHQSLNYRTPWEV
                     YSGEKAKKVNIK"
BASE COUNT          295 a          310 c          365 g          243 t
ORIGIN      
        1 agcggaattc aagacaaaag ttgtgcttga gatgcttcga gaagaagaaa cgctcagtca
       61 gatcgcggcc aggcataaaa tccatcacac agttctgcag aactggaaga agacggtcac
      121 cgaaggaatg ccgggcctgt tcgccgatcc ccgaaagaag agcgcggagg atctgcagaa
      181 ggattctacg atgaacgacc tctacaagca agtcgggctg ctctccatgc agctcgaatg
      241 gctcaaaaaa aaatgcggtg tcgactcttt ctcttcatga acgcagggcg ctggtggact
      301 gggagaacaa ggatttcccc ctctcctggc aggcctggct gctcggactc aacagaacag
      361 gactctacta cgttcccggg cctctgtccg aggaggtgct gctgctccgc aggcggatag
      421 acgagattca caccgaccag ccgtgctacg gctcgaggag ggtgcaggcg gtgttgtcgc
      481 ggggaggact gccggtatcg cgcaagcgga ttcagcgtct catgagagac atgggaatcc
      541 tggcgatata tcccggtccg aaactgagcg cgggcgacga gagtccgaag ttcccctatc
      601 ttctgcgggg cgtggacatc gaccatccga atcacacgtg gggcaccgac gtgacctacg
      661 tgcggctgaa ggggggattc atgtatctga cggtgtttct tgactggtac tctcgctacg
      721 tcgtttcgtg ggcattttcg gattcgctcg cggccgggtt cgtggtcgag gcgctggacg
      781 agggattgaa ggtcgcccgt ccgaggatca tcaacagcga tcagggctcc cagtacacga
      841 gcaaccttta catcgagcgg atccgcgccg agagcgaaag tatctccatc agcatggacg
      901 gaagggggcg ttgcatggac aacatcttca cggagaggtt ctggaggagc tacaagcagg
      961 aggaggtgta tctgaaggag tacgagagcc ctcgcgaagc ccgccgctcg acggcggagt
     1021 acatgaagca ttataatttc aaccggcccc atcagtcgtt gaattaccgg acaccctggg
     1081 aggtgtactc gggggaaaag gccaaaaagg ttaacattaa atagtcgcaa ttatttcccg
     1141 cacgagttaa aaatccaatg attttttgtc ttgacaaccg ggtccacttt agatagtctc
     1201 cgatagaaga act
//
LOCUS       DAOTIL010000948         1211 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminobacteriaceae bacterium isolate AMR_MDS_3026
            NODE_137969_length_1211_cov_3.580450, whole genome shotgun
            sequence.
ACCESSION   DAOTIL010000948 DAOTIL010000000
VERSION     DAOTIL010000948.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868050
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminobacteriaceae bacterium (wastewater metagenome)
  ORGANISM  Aminobacteriaceae bacterium
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae.
REFERENCE   1  (bases 1 to 1211)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1211)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:19:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,224
            CDSs (total)                      :: 3,198
            Genes (coding)                    :: 3,184
            CDSs (with protein)               :: 3,184
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1211
                     /organism="Aminobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_137969_length_1211_cov_3.580450"
                     /isolate="AMR_MDS_3026"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:3034609"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            195..1172
                     /locus_tag="P5201_14745"
     CDS             195..1172
                     /locus_tag="P5201_14745"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRV99855.1"
                     /translation="MKGKKKSHVKATLKDAPTETTEKGERRKIDEHWKAIIKKFFLLV
                     LRRVLPELADDLDTSRKVVFMEKELEELTVYVDGPKQIPDILARVPTASGRDVWLILH
                     VEIQGPEGGDLPERMFFYNSSLRVTHLKKRSDVTDAVSCAILTAKRPCGEEEMYLRES
                     YRNRVSYEYPSLKLWELDADELESSNNPFDWALYAGKCALESDRSETLKLDYLTRLIE
                     KLDAKGWTHDEKLALFRFMDALLHPKSPELRKAYDDFREQRRKEGKIVLLSVMEERAQ
                     ERGKKIGEAKKALAVASEMLDAGESAEKILRYSKISRKELDKLVAARRN"
BASE COUNT          292 a          308 c          398 g          213 t
ORIGIN      
        1 gtttgccgca tccactcata gtctgcttcg gctctcgtca ggccacataa aagcaggact
       61 tgaagaaggc cttgtatgaa gctatactgt tctcgtgcaa caagacgcgc aatactctcc
      121 ggcggcgctt ccggctgtcg aaaacatgaa gggggccgaa cgccatcgca cgcgcttcga
      181 aggggtccgc cgaaatgaag gggaaaaaga agagccacgt caaggcgacg ctaaaggacg
      241 ccccgacgga gacgactgaa aaaggggaac gcaggaagat cgacgaacac tggaaggcga
      301 tcataaagaa gttttttctc ctcgtccttc ggagggttct tcccgagctg gccgacgatc
      361 tggacacctc gcggaaggtg gtcttcatgg agaaggagct tgaggagctt actgtgtacg
      421 tcgacgggcc gaagcagata cccgacattc tcgcccgcgt tccgaccgcc tcggggcgcg
      481 acgtgtggct gatcctccac gtggaaatac aggggcctga ggggggcgac ctgccggagc
      541 ggatgttttt ctacaactcc tctcttcggg tgacgcacct caagaagcgg agcgacgtta
      601 cggatgcggt atcctgcgca atactgacgg cgaaacggcc ctgcggtgaa gaggagatgt
      661 accttcggga gagctacagg aaccgggttt cgtacgagta tccgtcgctg aagctgtggg
      721 agctggacgc agacgagctt gagagcagca acaacccgtt tgattgggcg ctctacgccg
      781 gaaagtgcgc gcttgagagc gaccggagcg aaacgctcaa actggattac ctgacgagac
      841 tgatcgagaa actcgacgcg aagggatgga cgcacgacga aaagctggcg ctcttccgtt
      901 tcatggacgc gctgctgcat ccgaagagtc cggaactccg gaaggcgtac gatgatttca
      961 gagaacagcg aaggaaggag gggaagatcg tgttgttgag cgtgatggag gaaagagcgc
     1021 aggagagagg caagaagatc ggcgaggcga agaaagcgct cgccgtggcc tccgagatgc
     1081 tggatgcggg cgagtctgcg gagaagattc tgcgatactc gaagatctcc cggaaggaac
     1141 tcgacaagct ggtcgcggca cgccggaact gatggggagt gctgcttctc gctcctcgga
     1201 gtgcgtacac t
//
LOCUS       DAOTIL010000949         1209 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminobacteriaceae bacterium isolate AMR_MDS_3026
            NODE_138298_length_1209_cov_4.068458, whole genome shotgun
            sequence.
ACCESSION   DAOTIL010000949 DAOTIL010000000
VERSION     DAOTIL010000949.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868050
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminobacteriaceae bacterium (wastewater metagenome)
  ORGANISM  Aminobacteriaceae bacterium
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae.
REFERENCE   1  (bases 1 to 1209)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1209)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:19:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,224
            CDSs (total)                      :: 3,198
            Genes (coding)                    :: 3,184
            CDSs (with protein)               :: 3,184
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1209
                     /organism="Aminobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_138298_length_1209_cov_4.068458"
                     /isolate="AMR_MDS_3026"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:3034609"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(164..>1209)
                     /locus_tag="P5201_14750"
     CDS             complement(164..>1209)
                     /locus_tag="P5201_14750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012870209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015079 - potassium ion transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="TrkH family potassium uptake protein"
                     /protein_id="HRV99856.1"
                     /translation="LGGMGIIVLGLAILPFLGVGGMELYKAEVPGPIPEKMTPRIQQT
                     ALYLWGVYVLLSALQTALLMLGGMNLFESLTHTFGTMATGGFSPLNKSIGQYNSAYFD
                     WVITIFMFLAGVNFVLHYRFLLGRWGAFWRDEEFRFYTVLTVFCTLTVTFVLRLHGTY
                     DSFVDALRFGAFQVVSIITTTGYVTADYELWPTYTQFILLLLMFVGACAGSTGGGIKN
                     LRLMVLARHVRAELSSILHPKAIVHVRVGGKPVGREIIASVTSFFILYIAIFTAGTLF
                     MTILDLDLITAMSSVAATLGNIGPGLGDVGPMQNYAAIPDSGKWVLSLCMMMGRLELY
                     TVVLLLLPETWKR"
BASE COUNT          272 a          369 c          367 g          201 t
ORIGIN      
        1 cgttctccgc gttgacgaaa cgcgcgcgca gtccaggaag aatctgctcg ttctccagac
       61 ggttcatgtc gatgagcttc acgaaaatcc cctccgattc gtcgttttga tgcagcctct
      121 ttcttcttcc gattctatca cagcgcggcg aagaaacggc cccctaccgc ttccacgtct
      181 cggggagcag caggaggacg acggtgtaca gctcgagccg ccccatcatc atgcagagcg
      241 agagcaccca ctttcccgag tccggaatgg cggcgtagtt ctgcatggga ccgacgtcgc
      301 cgaggccggg gccgatgttc ccgagcgtcg ccgcgacgct cgacatggcg gtgatcagat
      361 cgaggtcgag gatggtcata aaaagagttc cggcggtaaa aatcgcgata tagaggataa
      421 agaaagaggt taccgaagcg atgatctcgc gccccacagg cttccccccg acacggacgt
      481 gcacgatggc cttcgggtga agaatggagg agagttcagc ccggacgtgc cgcgcaagca
      541 ccataagccg gagattcttt atgccgccgc cggtggagcc cgcgcatgcg ccgacaaaca
      601 tcagcagcag caggatgaac tgcgtatagg tcggccacag ctcgtagtcg gcagtgacgt
      661 accccgtcgt ggtgatgatg ctcaccacct ggaaggcgcc gaagcgcagg gcatccacaa
      721 aagagtcgta tgtaccgtgc agacgcaaca cgaacgtcac cgtcagcgtg cagaaaacgg
      781 tcaggacagt atagaaccgg aactcctcgt ccctccagaa cgccccccag cgacccagca
      841 gaaaacggta gtggaggacg aagttcaccc ccgcgaggaa catgaagatg gtgatcaccc
      901 agtcgaagta cgcggagttg tactggccga tgctcttgtt cagcggcgag aagccgcccg
      961 tcgccatcgt gccgaaggtg tgcgtcagcg actcgaagag gttcatccct ccgagcatca
     1021 ggagcgccgt ctggagcgcc gagagcagca cgtagacccc ccacagatag agcgcggtct
     1081 gctggattct cggggtcatc ttctcgggga tcgggccggg aacctccgcc ttgtagagct
     1141 ccatccctcc gacgccgagg aagggaagga tcgccaggcc gaggacgatg atccccatgc
     1201 cgccgagcc
//
LOCUS       DAOTIL010000950         1209 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminobacteriaceae bacterium isolate AMR_MDS_3026
            NODE_138403_length_1209_cov_2.106586, whole genome shotgun
            sequence.
ACCESSION   DAOTIL010000950 DAOTIL010000000
VERSION     DAOTIL010000950.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868050
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminobacteriaceae bacterium (wastewater metagenome)
  ORGANISM  Aminobacteriaceae bacterium
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae.
REFERENCE   1  (bases 1 to 1209)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1209)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:19:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,224
            CDSs (total)                      :: 3,198
            Genes (coding)                    :: 3,184
            CDSs (with protein)               :: 3,184
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1209
                     /organism="Aminobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_138403_length_1209_cov_2.106586"
                     /isolate="AMR_MDS_3026"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:3034609"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1209)
                     /locus_tag="P5201_14755"
     CDS             complement(<1..>1209)
                     /locus_tag="P5201_14755"
                     /inference="COORDINATES: protein motif:HMM:NF037694.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRV99857.1"
                     /translation="REVARGTALLSSEGIASFSARLAPASPTGMYSAVIALPGGENAP
                     LGRTEFFVEDFVAPRLEVKAVPDASFLTAGDTLTIDVSSSYLFGAPASGLVFEGEVRA
                     VAAPFRPEGWNAFTFGDMERTFESMTDFVGSGTLDETGKGTLSFSGPEGWLPPAAIDL
                     SFFVRVMEESGRWVPTSVRLPFHPYPVYLGIESPKGDIVPGKEIAVRVAAVTPEGKAS
                     DLEIAVAEVFLVQRHYNLVRTGNQTRMQMQRELVPHAQGGVEFADGIGSFSFTPKIDG
                     EYVVKISDETSNSMASATLYAWTPYGGGASGSTLLDRVVVTADKKSYEPGEKATVTLR
                     SPFEGNLLVTVETNRELFRKVVSLKKGEVNLQVPVTDDMIPNAYITASVIRPVVGRLH
                     EAWAAREPVVV"
BASE COUNT          232 a          369 c          384 g          224 t
ORIGIN      
        1 caccaccacg ggctcgcgcg ccgcccacgc ctcgtggagg cgcccgacga cgggccggat
       61 cacggaagcc gttatatagg cgttcggaat catgtcgtcg gtgacgggaa cctgaagatt
      121 cacctcgcct ttcttcagag agacaacttt tcggaaaagt tccctgtttg tttcgacggt
      181 aaccaggagg ttcccctcga aggggcttcg gagcgtgact gtcgcctttt ctcccggttc
      241 gtagctcttc ttgtccgcgg tcaccacaac gcggtcgagc agcgtggagc cgcttgcgcc
      301 gccgccgtag ggagtccaag cgtagagcgt cgccgacgcc atgctattgg acgtttcgtc
      361 ggagattttg acgacgtact cgccgtcgat cttcggtgtg aaggagaacg agccgatgcc
      421 gtccgcgaat tccacgcccc cttgagcgtg cgggacaagt tcccgctgca tctgcatcct
      481 cgtctggttg cccgtacgga cgaggttata gtggcgctgg acaaggaaaa cctcggcaac
      541 tgcgatttcg agatccgatg ctttgccctc cggcgttact gccgcaactc ggaccgcgat
      601 ctccttaccg ggaacgatat cccctttagg cgactcgata cccaaataga cggggtaggg
      661 gtggaagggg agcctcacgg aggtcgggac ccatcgtccg ctttcctcca tcaccctgac
      721 gaagaatgag agatcgatag ccgccggggg aagccagcct tcaggtccgg agaaggagag
      781 cgttcccttc cccgtctcgt cgagtgtgcc ggacccgacg aagtccgtca tgctctcgaa
      841 cgtgcgctcc atatcgccga aggtgaacgc gttccaccct tcggggcgga acggcgctgc
      901 cacggcgcgc acctcaccct cgaagacgag tccggacgca ggagcgccga agagatacga
      961 cgaggagacg tcgatggtca gcgtatcccc agcggtcagg aaggaggcgt cggggacggc
     1021 cttcacttcg aggcggggag cgacgaagtc ctccacgaaa aactcggttc ttccaagagg
     1081 cgcgttttct cctccgggaa gggcaatcac cgcgctgtac atgcctgtgg gggaagcggg
     1141 cgcaagacgg gcggagaagg aggctatccc ttcggaagag agcagcgcgg ttccgcgcgc
     1201 gacttcgcg
//
LOCUS       DAOTIL010000951         1209 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminobacteriaceae bacterium isolate AMR_MDS_3026
            NODE_138433_length_1209_cov_1.691508, whole genome shotgun
            sequence.
ACCESSION   DAOTIL010000951 DAOTIL010000000
VERSION     DAOTIL010000951.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868050
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminobacteriaceae bacterium (wastewater metagenome)
  ORGANISM  Aminobacteriaceae bacterium
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae.
REFERENCE   1  (bases 1 to 1209)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1209)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:19:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,224
            CDSs (total)                      :: 3,198
            Genes (coding)                    :: 3,184
            CDSs (with protein)               :: 3,184
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1209
                     /organism="Aminobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_138433_length_1209_cov_1.691508"
                     /isolate="AMR_MDS_3026"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:3034609"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..698
                     /locus_tag="P5201_14760"
     CDS             <1..698
                     /locus_tag="P5201_14760"
                     /inference="COORDINATES: protein motif:HMM:NF014661.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="YcaO-like family protein"
                     /protein_id="HRV99858.1"
                     /translation="SVTASTMGARGKGIPGAVFRAALPETIPFPKVQELFKKIRSAGV
                     EVLLCDNGTDVGVPTWNCYLFDMEDSPGEIGGVAHGMGSSLDHETAMIRAVTEAVQGR
                     CVFLSGVRDIVPSKEFAGFMNVGARTALKAMKSSVGEWLDMSGVKDVPFATFEEDVHE
                     TVRRLSAVGLDRVIVYRLSRPDDPVQAVKVVVPGAEGYILPYYQPGERGVAAIRRGFE
                     GGSPLFRTEGFSW"
     gene            692..>1209
                     /locus_tag="P5201_14765"
     CDS             692..>1209
                     /locus_tag="P5201_14765"
                     /inference="COORDINATES: protein motif:HMM:NF019429.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TfuA-like protein"
                     /protein_id="HRV99859.1"
                     /translation="MVAVIFGGPSLEGVDRTQSSALFLPPAVQGDLLRAVREHRPSVI
                     GLLDCDLPYRSLPAWHKEILFALSRGIAVVGAAAVGAHRAAELAPNGMEGVGGIYERV
                     ASGELERDDEVLCDWEETPDGIRRLTLPLVTMRAALSAAKDAGVLSGRSAASLLSHAE
                     GLYRVQLQGVRQG"
BASE COUNT          209 a          369 c          416 g          215 t
ORIGIN      
        1 actccgtgac cgcatccacc atgggggccc gcggcaaggg gatccccggc gcggtctttc
       61 gcgccgccct gccggagacg attccgtttc cgaaggtgca ggagctgttc aaaaaaatac
      121 gctcggccgg tgtcgaggta cttctctgcg acaacgggac tgacgtcggc gtgccgacct
      181 ggaactgcta cctcttcgat atggaggact ccccggggga gataggcggg gtggcccacg
      241 gaatgggaag ctctctcgac catgaaaccg cgatgatccg tgctgtaacc gaggctgttc
      301 aggggaggtg cgtcttcctc tccggtgtgc gcgacatcgt cccgtccaag gagttcgccg
      361 gattcatgaa cgtcggtgcg cgcaccgcgt taaaggcaat gaagagctcc gtcggcgaat
      421 ggctggacat gagcggtgtc aaggacgttc ccttcgccac gttcgaggag gacgttcacg
      481 agacggtccg gcggctctcg gccgtggggc tcgaccgggt gatcgtctac cgcctttccc
      541 gtcccgacga tccggtccag gccgtgaaag tggtggttcc cggagcggag gggtatatcc
      601 tcccgtacta tcagccggga gaacggggcg tcgcggcaat ccgccgcgga ttcgaaggag
      661 gaagcccact cttcagaacg gagggattct catggtagcg gtcatcttcg gcggtccctc
      721 cctggagggc gtcgacagaa cacaatcgag cgcgctcttc ctgccgcccg ccgtacaggg
      781 cgatcttcta cgggcagttc gcgaacatcg cccgtcggtg atagggctgc tcgactgcga
      841 tctgccctac cggagcctgc cggcatggca caaggagatc ctcttcgctc tctcccgcgg
      901 gatcgcggtc gtgggtgccg ctgcggtcgg ggcgcacagg gccgccgagc tggcgccgaa
      961 cggcatggag ggagtcggcg gaatctacga acgtgtggct tcgggcgaac tggaacgcga
     1021 cgacgaagtg ctctgcgact gggaagaaac accggacggc atccggcgcc tcacactgcc
     1081 gctggtgacg atgcgcgccg ctctgtcggc ggcgaaggat gccggagtcc tctcagggag
     1141 gagtgccgct tcgctgctct cccacgcgga agggctatat cgagtgcagc ttcaaggcgt
     1201 gcggcaagg
//
LOCUS       DAOTIL010000952         1206 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminobacteriaceae bacterium isolate AMR_MDS_3026
            NODE_138870_length_1206_cov_3.013901, whole genome shotgun
            sequence.
ACCESSION   DAOTIL010000952 DAOTIL010000000
VERSION     DAOTIL010000952.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868050
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminobacteriaceae bacterium (wastewater metagenome)
  ORGANISM  Aminobacteriaceae bacterium
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae.
REFERENCE   1  (bases 1 to 1206)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1206)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:19:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,224
            CDSs (total)                      :: 3,198
            Genes (coding)                    :: 3,184
            CDSs (with protein)               :: 3,184
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1206
                     /organism="Aminobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_138870_length_1206_cov_3.013901"
                     /isolate="AMR_MDS_3026"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:3034609"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>1206
                     /locus_tag="P5201_14770"
     CDS             <1..>1206
                     /locus_tag="P5201_14770"
                     /inference="COORDINATES: protein motif:HMM:NF012581.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="proton-conducting transporter membrane subunit"
                     /protein_id="HRV99860.1"
                     /translation="ISAFGGAAAVWTTFSSLSDSDSGSDAEQWRLCGLFFILWASLNG
                     ILTTGDLFNLFIFYEILAVAAYVLTGASKTREALEAGLKYLVLGTVGALLILLATSML
                     YMGGGILNFALLSSVVGGIPRGALAVAAGFMIAGLGLKMGMVPFHFWLPDAHSSASTG
                     VSMLLSGVVVKASFYSLLRVTTLLFPAEQAGFSIPDTLLVLGLLSLFTGHISAQRQTS
                     IKRMLAWSTVAHMGYLLIGIGCASPLGVAGALLHSFNHAAAKSALFLGAGSFSTLSGS
                     KEWRSFTGFGRRFPREGAAFLLLAASIIGLPPLGGFWGKWMIVLAAMEKGRWMTAVAV
                     ELGTALSCVYYVPVLFRLLSPRRNEEAVPRGVFHSSALFIALAMTPLLLAFFYFVPAL
                     HEYLITVGG"
BASE COUNT          169 a          346 c          365 g          326 t
ORIGIN      
        1 tcatctccgc tttcggagga gctgccgccg tatggacgac tttttcgagc ctctccgact
       61 ccgactccgg gtcggatgcg gagcagtggc gtctctgcgg tctttttttc atcctctggg
      121 cttcgctcaa cgggattctt acaaccggcg atctcttcaa cctctttatt ttttatgaaa
      181 ttcttgccgt agccgcctac gtccttacag gcgcctcgaa aacgcgggag gccctggaag
      241 ccgggctcaa ataccttgtt ctggggacgg tcggcgcgct tttgattcta ctcgcgacga
      301 gcatgctgta catggggggc ggaatactga atttcgcgct gctctcttca gttgtcggcg
      361 gcattccgcg gggagcgctc gccgtcgcgg ccggattcat gattgcggga ctgggactca
      421 agatgggcat ggtcccgttc catttttggc tgcctgatgc tcattcgtcg gcttcgacgg
      481 gagtgagcat gctcctctcc ggcgttgtcg tcaaggcgtc tttttattct ctgctcaggg
      541 tgacgacgct gctctttccg gctgaacagg caggtttttc aataccggat acacttcttg
      601 tgctggggct gctctcgctc ttcaccgggc atatttcggc gcagcggcag acgagcatca
      661 agcgcatgct tgcctggtcg acagtggcgc acatggggta tctgctgatc ggcatcgggt
      721 gcgcgtctcc tctgggagtg gccggggcgc tccttcattc gttcaaccac gctgcggcaa
      781 aatcggcgct cttcctcgga gccggaagtt tttcgacgct ctccggcagc aaggagtggc
      841 gttcctttac cggtttcgga cgacgttttc cccgcgaagg cgcggcgttt cttctgctcg
      901 cggcgtcgat catcggactg ccgccgctgg gcggtttttg gggcaagtgg atgatcgtcc
      961 ttgcggcgat ggagaagggg cgctggatga ccgccgtcgc agtggagctt ggaacggcgc
     1021 tctcctgcgt ctattatgtt cccgtgctct ttcgtctgct ttcgcctcgc cggaacgagg
     1081 aggcggttcc aaggggcgtt tttcactcca gcgcgctctt tattgcgctg gcaatgacgc
     1141 ctcttcttct tgcatttttt tacttcgtgc cggcgttgca cgagtacctt atcacggtag
     1201 gcgggt
//
LOCUS       DAOTIL010000953         1206 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminobacteriaceae bacterium isolate AMR_MDS_3026
            NODE_139009_length_1206_cov_1.286707, whole genome shotgun
            sequence.
ACCESSION   DAOTIL010000953 DAOTIL010000000
VERSION     DAOTIL010000953.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868050
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminobacteriaceae bacterium (wastewater metagenome)
  ORGANISM  Aminobacteriaceae bacterium
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae.
REFERENCE   1  (bases 1 to 1206)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1206)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:19:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,224
            CDSs (total)                      :: 3,198
            Genes (coding)                    :: 3,184
            CDSs (with protein)               :: 3,184
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1206
                     /organism="Aminobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_139009_length_1206_cov_1.286707"
                     /isolate="AMR_MDS_3026"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:3034609"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..570
                     /locus_tag="P5201_14775"
     CDS             <1..570
                     /locus_tag="P5201_14775"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRV99861.1"
                     /translation="ETPLDCTAWTDVTCRRHAVHFFSGERAEKERGAPGFRENTRRRR
                     CPLSGIGEYRQNMFLLLEMSVLGPLPCHLIYLFAVPAAEMSQLFRNTLYMREVAHSLT
                     ASLLEERFSPFLVATDHLPLLLTQYCLVAGRFSTAFWLVAFANLQTVLRETLLAGIEH
                     DEAVRAINGLLNMLFLIPLAAILLRAFGA"
     gene            636..1016
                     /locus_tag="P5201_14780"
     CDS             636..1016
                     /locus_tag="P5201_14780"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRV99862.1"
                     /translation="MNEREFTLEEVASMVSGVLLVEKGRMLVDIACFMLEREVDPEEI
                     PYVGREIRRRILQQHHSLRAIGEIGEMDSLEEVAECVRRLKEAYGETFVLKAREREEK
                     PLFKKLLDCTRNALFKKDRMERAK"
BASE COUNT          257 a          378 c          353 g          218 t
ORIGIN      
        1 gaaacgccgc tcgactgtac ggcatggacc gacgtaacct gtcgacggca tgccgtacac
       61 ttcttttccg gagagcgcgc cgagaaagag cgcggcgcgc cgggattccg ggaaaacaca
      121 cgaaggaggc gttgcccgtt gtccggtatc ggtgaataca ggcaaaacat gttcctgttg
      181 ctggaaatgt ccgttctggg accgttgccg tgccatctga tctatctctt cgccgtgccc
      241 gcggccgaga tgagccagct cttccgcaac acgctctaca tgcgggaagt cgcccactcc
      301 ctgaccgcct cgctgctcga agagcgcttc tccccctttc tggtggcaac cgaccacctg
      361 ccgctgctcc tcacccagta ctgcctggtc gcgggaagat tttcgaccgc cttctggctc
      421 gtcgccttcg ccaacctcca gaccgtgctc cgcgagacac ttctcgccgg tatagagcac
      481 gatgaagcgg tccgcgcgat caacggcctt ctcaacatgc tctttctcat cccgctggcc
      541 gcgatcctcc tgagggcgtt cggcgcgtga aggaacggcc tccccccgga ggccggaacg
      601 gaattctttt gtgctgaaag aaaggaaggt gcggaatgaa cgaacgcgaa ttcacgctcg
      661 aagaagtcgc ctccatggtg tcgggagttc ttctggtcga gaaggggcgt atgctggtcg
      721 acatcgcctg ctttatgctg gagcgcgagg tcgatcccga agagatcccc tacgtcggga
      781 gagagatacg aaggcgcatc ctgcaacaac accactccct gcgggcgatc ggggaaatcg
      841 gcgagatgga cagcctcgaa gaagtcgccg aatgcgtccg gaggctcaaa gaagcgtacg
      901 gagaaacgtt cgttctgaaa gcccgcgagc gagaggagaa gccgctcttc aaaaagctcc
      961 tcgactgcac gcggaacgcg ctcttcaaga aggaccgcat ggaacgggcg aagtaaaaaa
     1021 tacccgcccc gccgaccgaa cgccgactga aaaaagactc ccgaagcgca tcttcgagac
     1081 ggtttcttcg tcggcgttcg ggctccgtcg taagtggtcg ctcctccgtc gtacactcct
     1141 cttgaacagc ccgacgcgcg ccgcacgcga caaacgcgcc gggaaacgaa ggaggtccgc
     1201 gaaatg
//
LOCUS       DAOTIL010000954         1201 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminobacteriaceae bacterium isolate AMR_MDS_3026
            NODE_139774_length_1201_cov_3.243455, whole genome shotgun
            sequence.
ACCESSION   DAOTIL010000954 DAOTIL010000000
VERSION     DAOTIL010000954.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868050
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminobacteriaceae bacterium (wastewater metagenome)
  ORGANISM  Aminobacteriaceae bacterium
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae.
REFERENCE   1  (bases 1 to 1201)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1201)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:19:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,224
            CDSs (total)                      :: 3,198
            Genes (coding)                    :: 3,184
            CDSs (with protein)               :: 3,184
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1201
                     /organism="Aminobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_139774_length_1201_cov_3.243455"
                     /isolate="AMR_MDS_3026"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:3034609"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>1201
                     /locus_tag="P5201_14785"
     CDS             <1..>1201
                     /locus_tag="P5201_14785"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HRV99863.1"
                     /translation="KKLSSEDIVIYLSDLLSPSGKRLSVEENRTFVVGATDAPVFSCW
                     DTLLSYGFVGGKVVHGRSQGEIAASLALQILDGKRAEDIPVVFDSPNRYAFNASALKT
                     FGVPESLLPPYSFLLQTTGDGLAATWDELVVRRFFSEELFSRHGAPMLIIDPESGLIL
                     AGNQAAHSFYGYPELQGMTIGSINALSSQQVQDEMQKATRLRKNHFDFRHRLADGSVR
                     DVRVYSYPIELDGREVLFSMVIDETERTIAEASLHRKERSFITVLSVAVLLQLAALFL
                     LARSAARRKKAEKELAEAYENQSILLDNIPTQIWLLTDDHTYGAVNRAHADFNGMRAE
                     EMSFRDMYEFLPGEVVEVCRRSNAEVFADARPVRTDEKIRDASGEERLLSIWKTPRLR
                     ADGSVEYV"
BASE COUNT          247 a          346 c          363 g          245 t
ORIGIN      
        1 gaagaagctt tcatccgaag atatcgtcat ctacctctcc gaccttctct ccccttcagg
       61 gaaaaggctc tcggtcgagg agaacagaac gttcgtcgtc ggggcaacgg acgcccccgt
      121 cttcagctgc tgggatacgc tgctttcgta cggtttcgtc ggcggaaagg tggtgcacgg
      181 gcggtctcag ggggagatcg ccgcgtcgct tgcgctgcag attctggacg gaaaaagggc
      241 ggaggatatc ccggtcgttt tcgacagtcc caaccggtac gccttcaacg cctccgcgct
      301 caaaacgttc ggcgtacccg agtcgcttct tccgccgtat tcgtttcttc ttcaaacaac
      361 cggggacgga ttggccgcga catgggacga gcttgtcgtc cgccgcttct tcagcgaaga
      421 gctcttctcc agacacgggg cgcccatgct catcatcgat ccggagagcg gactcattct
      481 ggcagggaat caggcagcgc acagcttcta cggttacccg gagctacagg ggatgacaat
      541 cggcagcatc aatgcactct cttcgcagca ggtgcaggac gagatgcaaa aagcgaccag
      601 gctgcggaag aatcacttcg attttcgcca caggcttgcc gacggttcgg tgcgcgatgt
      661 acgggtttac tcctacccga tcgagctcga cgggagagag gtactcttct ccatggtgat
      721 cgacgaaacg gagaggacga tcgcagaagc ctctcttcat cgcaaggagc gttcgtttat
      781 cacggtgttg tccgttgcgg tgctcttgca gcttgcggcg ctctttctcc ttgcgagaag
      841 cgccgcgcgg agaaagaaag cggagaaaga gcttgcagaa gcctacgaaa accagagtat
      901 ccttctcgac aacattccga cgcagatctg gcttctgacc gacgaccaca catacggcgc
      961 ggtgaatcgt gcgcacgccg acttcaacgg gatgcgggcg gaggagatgt cgttcaggga
     1021 catgtacgaa tttctgccgg gcgaggtcgt cgaggtctgc cggagatcga acgcggaggt
     1081 ctttgccgac gcccgccctg tccgtaccga cgagaagatc cgcgacgctt ccggagaaga
     1141 gcggcttctc tccatttgga aaacgccgcg gctccgtgcc gacggcagtg tggagtatgt
     1201 c
//
LOCUS       DAOTIL010000955         1200 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminobacteriaceae bacterium isolate AMR_MDS_3026
            NODE_140055_length_1200_cov_1.747598, whole genome shotgun
            sequence.
ACCESSION   DAOTIL010000955 DAOTIL010000000
VERSION     DAOTIL010000955.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868050
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminobacteriaceae bacterium (wastewater metagenome)
  ORGANISM  Aminobacteriaceae bacterium
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae.
REFERENCE   1  (bases 1 to 1200)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1200)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:19:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,224
            CDSs (total)                      :: 3,198
            Genes (coding)                    :: 3,184
            CDSs (with protein)               :: 3,184
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1200
                     /organism="Aminobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_140055_length_1200_cov_1.747598"
                     /isolate="AMR_MDS_3026"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:3034609"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            158..397
                     /locus_tag="P5201_14790"
     CDS             158..397
                     /locus_tag="P5201_14790"
                     /inference="COORDINATES: protein motif:HMM:TIGR01439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB/MazE/SpoVT family DNA-binding
                     domain-containing protein"
                     /protein_id="HRV99864.1"
                     /translation="MIITASEKGRIVIPAIIRRQLKMQAKTKLFVQVRNGEIVLTPAK
                     DVKSLYGALAEYAVPGSTEEDEREAMYRAVAESNE"
     gene            482..799
                     /locus_tag="P5201_14795"
     CDS             482..799
                     /locus_tag="P5201_14795"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRV99865.1"
                     /translation="MLDVEKGDLSVWCESVILAEVVWVLSSFYKVERVKITEFLLPLI
                     QMEHFLMAEKDRMIHALYLFSGSVPHFGDACLCAAALDISEGRILSFDKKLKNVSGIV
                     CKG"
     gene            911..1129
                     /locus_tag="P5201_14800"
     CDS             911..1129
                     /locus_tag="P5201_14800"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRV99866.1"
                     /translation="MYISLMERKGMKIGREEGREEGREEGILIGTEQNKRDNAIRMLR
                     KGFGTADVSECVDLPEEEVRRLAEEMKS"
BASE COUNT          283 a          292 c          365 g          260 t
ORIGIN      
        1 ccgcactcct cggcggcctc gctccctgcg acgggaacat gagcatcgtc cactccgaca
       61 ggggcgacca ccagctcctt ctctccgtcc ttccggaagt ggcgaaagcc gtcgattcgt
      121 tgcggcactt ggctgcgcac aacgaggggg tataaaaatg ataattacag cctcggaaaa
      181 ggggcgaatc gtgatacctg caataattcg caggcagctc aagatgcaag cgaaaacaaa
      241 attgtttgtt caggtgcgga acggggagat agtgttgacg ccggccaagg atgtgaagag
      301 tctttacggt gcgctcgccg agtatgctgt tcccggaagc accgaagagg atgagcgcga
      361 agcgatgtac cgggctgtgg cggaatcgaa tgagtgattt gtactgcatc gatgcgaata
      421 tcattctgcg gtttcccttc tcggggataa tgaaaaactg agcccggctg cggaggctat
      481 tatgttggac gtcgagaagg gagacttgtc cgtctggtgt gaatcggtca ttcttgccga
      541 ggttgtgtgg gttctctcat ctttctacaa ggtcgaacgc gtcaagataa ccgaatttct
      601 acttcctctt attcagatgg aacattttct aatggcggaa aaagatcgca tgatacatgc
      661 tttgtatcta ttcagcggtt ctgttcccca tttcggagat gcctgccttt gtgcggctgc
      721 tttggacatt tccgaaggcc gtattctatc gttcgacaag aagctgaaaa acgtatcggg
      781 gatcgtatgc aaggggtaag cggaacgcgg aactccggcg cctcgcccgc ttcacccggt
      841 gctattccgg aagtcctccg gacgtagagg cttcgtcgac attctcgaac gccgaaagga
      901 ggacgaagcg atgtacatca gccttatgga acgaaaagga atgaagatcg gcagggaaga
      961 aggcagagaa gaaggcagag aagaaggtat tctcatcgga acggagcaaa acaagcggga
     1021 caacgccatc aggatgctgc gcaaggggtt cggcacagca gatgtttccg agtgcgtcga
     1081 tcttcccgag gaggaggtcc gccgtctcgc cgaagagatg aagtcctgat cgcgatgggt
     1141 gttccgctcc gccggtggtg cggagagccg ggccgcggac gggcctcgcc gtaacagtgt
//
LOCUS       DAOTIL010000956         1197 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminobacteriaceae bacterium isolate AMR_MDS_3026
            NODE_140505_length_1197_cov_3.315236, whole genome shotgun
            sequence.
ACCESSION   DAOTIL010000956 DAOTIL010000000
VERSION     DAOTIL010000956.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868050
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminobacteriaceae bacterium (wastewater metagenome)
  ORGANISM  Aminobacteriaceae bacterium
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae.
REFERENCE   1  (bases 1 to 1197)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1197)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:19:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,224
            CDSs (total)                      :: 3,198
            Genes (coding)                    :: 3,184
            CDSs (with protein)               :: 3,184
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1197
                     /organism="Aminobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_140505_length_1197_cov_3.315236"
                     /isolate="AMR_MDS_3026"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:3034609"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1197)
                     /locus_tag="P5201_14805"
     CDS             complement(<1..>1197)
                     /locus_tag="P5201_14805"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRV99867.1"
                     /translation="LNSLVESFTRYGIPYSMREGATVSETALWKSALRALDLAADGWP
                     PVETADFLSSLLFAPFSFPRRAFGNALPSGKKGWTGFLEEFPPEAGRDAFRRATAFAD
                     VVQRGGRPEELLSAFAHLAPSRAELKLLVERAARFPRLDGHIREVSLAVQEANEKERS
                     LRELRRDLGDAGSARLSFEEAAAFLGQWAETASIWMPSPVSPTVAIHPGTPPSLVRAP
                     VWILPGMTAKRWPGQVHESPLLSDERKEFLHESLELGRSHLPLVPEKRSQREALFRRL
                     AACGESLCVLLRPLADDGGRPLPESPFVDDACSSSHPWLRSSGAEPLKRSLGEILAPE
                     EYPVAEGVETRWRSWATTGAARGSLKEIPLLPPKEKVFSLSDLDTYVSCPFRYYCART
                     ASLDTP"
BASE COUNT          203 a          406 c          423 g          165 t
ORIGIN      
        1 ggggcgtgtc cagcgacgcc gtgcgggcgc agtagtaacg gaaggggcat gaaacgtacg
       61 tgtccaggtc gctcagtgaa aacaccttct ccttcggagg gaggagggga atctctttca
      121 ggctgccccg ggccgcgccg gtcgtcgccc acgagcgcca gcgcgtctcc acgccctccg
      181 ccacagggta ctcctccggg gcgagaatct cgccgagcga gcgtttcagc ggctccgctc
      241 ccgaagagcg cagccacgga tgcgacgaag agcaggcgtc gtcgacgaag ggggattcgg
      301 ggagcggacg gccgccgtcg tcggccagag ggcgcagcag aacgcagagg ctctcgccgc
      361 aggccgcaag gcggcggaag agcgcctcgc gctggctccg cttctccggc accagcggca
      421 ggtggcttcg cccgagctca aggctttcgt gcaggaactc tttccgttcg tcgctcagaa
      481 gaggggattc gtgcacctga cccggccacc gcttcgccgt catcccggga agaatccaca
      541 caggggcgcg gacgagcgag gggggcgtgc cggggtgaat ggcgacggtc ggagagaccg
      601 gcgagggcat ccagatcgac gccgtctccg cccactgccc gaggaaggcc gcagcctctt
      661 cgaaagagag gcgcgcgctt cccgcgtcgc caaggtctct gcgcagttcg cgcagggaac
      721 gctccttttc gttcgcctcc tgcacggcga gcgacacctc gcggatatgc ccgtcgaggc
      781 gggggaagcg cgccgcccgt tcgacgagca gcttcagctc cgcgcgggag ggcgcaaggt
      841 gcgcgaaggc cgacaggagc tcctcagggc gcccgccgcg ctgcacgacg tcggcgaagg
      901 ccgtcgcccg gcggaacgcg tccctcccgg cctcgggggg gaattcctcc aggaagcccg
      961 tccacccttt tttgcccgaa ggcagcgcat tcccgaatgc ccgtcgcggg aagctgaacg
     1021 gagcgaagag cagcgacgaa agaaagtccg ccgtctccac ggggggccac ccgtccgcgg
     1081 ccaggtcgag cgcccgcagc gcgctcttcc agagcgccgt ttcggagacc gtcgctccct
     1141 cgcgcatcga atagggaatg ccgtaccgtg tgaagctctc gacgagggag ttcagct
//
LOCUS       DAOTIL010000957         1193 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminobacteriaceae bacterium isolate AMR_MDS_3026
            NODE_141256_length_1193_cov_3.253076, whole genome shotgun
            sequence.
ACCESSION   DAOTIL010000957 DAOTIL010000000
VERSION     DAOTIL010000957.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868050
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminobacteriaceae bacterium (wastewater metagenome)
  ORGANISM  Aminobacteriaceae bacterium
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae.
REFERENCE   1  (bases 1 to 1193)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1193)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:19:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,224
            CDSs (total)                      :: 3,198
            Genes (coding)                    :: 3,184
            CDSs (with protein)               :: 3,184
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1193
                     /organism="Aminobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_141256_length_1193_cov_3.253076"
                     /isolate="AMR_MDS_3026"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:3034609"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(278..712)
                     /locus_tag="P5201_14810"
     CDS             complement(278..712)
                     /locus_tag="P5201_14810"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRV99868.1"
                     /translation="MSRDGKFFLLLVGFAIFSLIAVSFGAIRSKKAAGSFEIHNIVLC
                     DEVDGGIKPVGVGSAFSYGTRQLCLWFEYSRAGDDENIRVRWYYRGRPIHSQEVVLDP
                     GSGAKAFCLLLEDGSPLPKGSYSVRISLFDRVFSDTFFEISE"
     gene            953..>1193
                     /locus_tag="P5201_14815"
     CDS             953..>1193
                     /locus_tag="P5201_14815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006300545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HRV99869.1"
                     /translation="MNTTRSKEVALKGLLIAAVTLATMLSMPVPGFRLYFNFGEGIIY
                     LAALLLGPVWGAAAGGIGAAMADLLLGYPLWAPFTL"
BASE COUNT          330 a          337 c          292 g          234 t
ORIGIN      
        1 ccgcaggagc gagcgccgtt tccacaacag cggtctcgga ggtggtgtcc gccgcgtcgt
       61 tcgcgatgag attatcggac atcatgaacg cagtcctaag cgtatttctt ttataaagcc
      121 aggagggctt tttgacacac ttcgggataa atgatcagag aaaaacacga acctggacat
      181 ccccttcctg aattgccccg tttcaaaagc ttcacgcgga aaagaacaca tgaaccggcg
      241 aaaacggacc aagttttgaa cttgtgaata gttgcagtta ctcggaaatt tcgaagaacg
      301 tatcggaaaa cactctgtcg aacagagaaa ttcggaccga atatgatcct ttggggagag
      361 gagagccatc ctcaagaagc aggcagaacg ccttggctcc gcttcctgga tcgagcacga
      421 cctcctgcga atgaatcggc cgccccctgt aataccagcg cacccggata ttctcgtcgt
      481 cgccggctct ggagtactca aaccataaac ataattggcg cgtgccataa gaaaaagcag
      541 accctacacc gacaggcttt attccgccgt ccacctcgtc gcataaaacg atattatgga
      601 tttcgaaaga acccgcagcc ttctttgaac gaatcgcccc gaacgagacg gcgatcaggg
      661 agaaaatagc gaaaccaacc agaagaagaa aaaatttccc gtccctggac attataccca
      721 ccgccccaga aaaaaaacaa gtgcagagaa aaaaaacatc gccgggtttc gacttccccg
      781 atcgtttgaa cagtccagaa tcagatggaa acagcctcca gctggctctt tccgaaagaa
      841 ctctaccacg aatagaatgg ggaggcaagc aggaaatgtc gggtgtatct ccggaccggc
      901 aaaaatcccg ctcccggcgg aaagcaatat atccccaagg aggttcaatc ggatgaatac
      961 aacacgctca aaagaagtcg ccctcaaagg gctcctcatc gccgcggtca cactggcgac
     1021 aatgctcagc atgcccgtac ccggattccg gctctacttc aatttcggag agggcattat
     1081 ctatcttgca gcgcttcttc tcggtcctgt atggggcgcg gcggcagggg gaatcggcgc
     1141 cgcgatggcc gacctgctgc tcggctaccc gctctgggcc ccctttacac tcc
//
LOCUS       DAOTIL010000958         1191 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminobacteriaceae bacterium isolate AMR_MDS_3026
            NODE_141751_length_1191_cov_1.655810, whole genome shotgun
            sequence.
ACCESSION   DAOTIL010000958 DAOTIL010000000
VERSION     DAOTIL010000958.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868050
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminobacteriaceae bacterium (wastewater metagenome)
  ORGANISM  Aminobacteriaceae bacterium
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae.
REFERENCE   1  (bases 1 to 1191)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1191)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:19:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,224
            CDSs (total)                      :: 3,198
            Genes (coding)                    :: 3,184
            CDSs (with protein)               :: 3,184
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1191
                     /organism="Aminobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_141751_length_1191_cov_1.655810"
                     /isolate="AMR_MDS_3026"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:3034609"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1168
                     /locus_tag="P5201_14820"
     CDS             <1..1168
                     /locus_tag="P5201_14820"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="PAS domain-containing sensor histidine kinase"
                     /protein_id="HRV99870.1"
                     /translation="QLALVARLVAGIRDSEEKYRLLAENVKDVIWMVSLPDFKFLYIS
                     PSVEELSGYSVEETMNMRLDEYLEPESLDHVKKLYGESLCRFRKGEIPEEGRVMEVRH
                     RRKDGSSVWLGVRSSYILNDDGEPVGILGISRDMTEIKHVQQELERANATKSTFLAII
                     AHDLRGPVGALGQTLELLEQNADAMDEEDLEEFLSLASGDARRVFSLLENLLTWSRSQ
                     LEQIVFRPVVWGVRIIVEESVELLASCASEKGIVLSNDAPPMMTARLDPGMMGVVLRN
                     LVGNALKFTSPGGSVSVSARRTDEGVEFAVRDTGRGIPPEIAEKLFRIDVGHGSTEGT
                     AGERGSGLGLILCKEFVEKHGGRIWFESSPGEGTVFFFSLPDRLNGDPAKERGV"
BASE COUNT          252 a          296 c          396 g          247 t
ORIGIN      
        1 gcagcttgcg cttgtggcca ggcttgtcgc cgggatacgc gacagcgagg agaagtaccg
       61 tcttctggcc gagaacgtca aagatgtgat atggatggta tcgttgcccg acttcaagtt
      121 tctctatatc agcccgtcgg ttgaagaact cagcgggtat tccgtcgaag agacgatgaa
      181 tatgcgcctc gacgagtatc tcgagccgga atcgctcgat cacgtgaaaa aactgtacgg
      241 cgaaagtttg tgccgcttcc gcaagggaga aatccccgaa gaaggaagag tcatggaggt
      301 tcgtcatcga cggaaagacg gctcgtccgt ctggctggga gtccggagca gctatatcct
      361 gaacgacgat ggcgaacccg tcggtattct tggaatttcg cgcgacatga cggagatcaa
      421 gcatgtgcag caagagcttg aacgcgccaa tgcgacaaaa agcacgtttc tcgccatcat
      481 cgcgcacgat ctgcgagggc ctgtcggcgc gttggggcaa acgctcgaac tgctggagca
      541 gaacgcggac gccatggacg aagaagattt ggaggaattt ctttctcttg caagcggcga
      601 cgcgcgaagg gttttttcgc tgcttgagaa tctgttgacc tggtccaggt cgcagctcga
      661 gcagatcgta tttcggcctg tcgtgtgggg tgtgaggata atcgtggagg agagcgtcga
      721 gttgcttgcc tcctgcgcat ccgagaaggg aatcgttttg tcgaacgacg ctccgcctat
      781 gatgaccgcc aggctggatc cggggatgat gggcgtcgtg ctgcgaaatc ttgtcggaaa
      841 cgcgctgaag ttcacttccc cgggcggcag tgtttccgtc tccgcgcggc ggacggacga
      901 gggcgtcgag tttgccgtcc gcgataccgg gcgcggcata cctccggaaa ttgcggagaa
      961 gctcttccgt atcgacgtcg gtcatggaag caccgaggga actgccggag agcggggtag
     1021 cggcctcggg ctgattctct gcaaggagtt cgtggagaag catggggggc ggatttggtt
     1081 cgagagctcg cccggagagg gaaccgtctt ctttttctcg ttgcccgaca gactgaacgg
     1141 cgatcctgcg aaagagcgcg gagtgtgacg gaacgaaagg aggtcgccga t
//
LOCUS       DAOTIL010000959         1190 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminobacteriaceae bacterium isolate AMR_MDS_3026
            NODE_141786_length_1190_cov_4.266079, whole genome shotgun
            sequence.
ACCESSION   DAOTIL010000959 DAOTIL010000000
VERSION     DAOTIL010000959.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868050
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminobacteriaceae bacterium (wastewater metagenome)
  ORGANISM  Aminobacteriaceae bacterium
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae.
REFERENCE   1  (bases 1 to 1190)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1190)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:19:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,224
            CDSs (total)                      :: 3,198
            Genes (coding)                    :: 3,184
            CDSs (with protein)               :: 3,184
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1190
                     /organism="Aminobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_141786_length_1190_cov_4.266079"
                     /isolate="AMR_MDS_3026"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:3034609"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1190)
                     /locus_tag="P5201_14825"
     CDS             complement(<1..>1190)
                     /locus_tag="P5201_14825"
                     /EC_number="2.5.1.15"
                     /inference="COORDINATES: protein motif:HMM:NF013007.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="dihydropteroate synthase"
                     /protein_id="HRV99871.1"
                     /translation="CCGTTPEHIAALVAAVRGIHPKKRAPVRGVPLASRSRLVLAGPG
                     HPFRIIGERINVSRKSPLRDEVERFVWTTLREEARLQTEAGAHVIDVNVGLPTIDQQA
                     AMAAAVSAAGQSTALPLSIDSDSALVLESGLGAVTGIPLINSFTAREDSLFPGLGLAK
                     RHGAAAVVLPIDENGLPESEEARKRVILKILESADSLGFPREGLIVDGLTLAVGADHA
                     APAVTLSALTFLREQGIASVLGVSNVSHGMPARSLLNRTFLAMAVAAGLDAAIMNPLD
                     HSMMETLAAAETLAGRDAGGRRFLGAAASFSAPSDAPRPAAHAAGQSAGDETLAVDPR
                     FLPLALSILRGDAQQAEANAQELLGSGTTPLDVVNKGVIPALEEVGKRYDSGAFFLPQ
                     LISS"
BASE COUNT          205 a          368 c          423 g          194 t
ORIGIN      
        1 cgacgagata agctggggaa ggaagaaggc gccgctgtcg tagcgcttcc ccacctcttc
       61 aagcgcggga atgactcctt tgttcacaac gtcgagcggg gttgttcctg agccgagaag
      121 ttcctgcgca ttcgcctccg cctgctgcgc gtcgccgcga agtatcgaaa gggcgagcgg
      181 caggaaccgg ggatcgacgg cgagcgtttc gtctcctgcg gactgacccg cggcgtgtgc
      241 cgcaggacgg ggcgcgtcgg aaggcgcgga gaacgaagcg gccgcaccga ggaagcgacg
      301 ccctcccgca tcgcgtcctg caagcgtctc cgctgcggcg agggtctcca tcatggagtg
      361 gtcgagcggg ttcataatgg cggcgtccag ccccgcggcg acggccatcg cgaggaacgt
      421 cctgttgagg agcgaacggg cgggcatgcc gtgcgagacg ttgctcacac ccaggacgga
      481 ggcgatcccc tgctctctga ggaaggtcag ggcggagagc gtgaccgccg gagcggcgtg
      541 atccgcgccg acggcaagcg tcagtccgtc gacgatgagg ccttcacgcg gaaatcccag
      601 agagtccgcg ctttcgagaa tcttaaggat gactctttta cgggcctctt cgctttcggg
      661 aagaccgttc tcgtcgatcg gaagcacgac cgccgcagcg ccgtggcgct ttgccagccc
      721 caagccgggg aagagggagt cctctctggc ggtgaaggag ttgatgaggg ggataccggt
      781 aacggccccg agccctgatt caagcaccag ggcgctgtcg ctgtcgatgg agagcggcaa
      841 cgcagtggac tggcctgcgg cggagactgc ggcggccatt gcggcttgct gatcgatcgt
      901 cggcaacccg acgttcacgt cgatgacatg cgcgcccgct tccgtctgca gccgcgcctc
      961 ctcgcggagc gtcgtccaga cgaagcgttc gacctcgtcc ctgagcgggg atttgcgcga
     1021 gacgttgatc cgctcgccga tgatgcggaa ggggtgtccc ggccccgcaa gcacgaggcg
     1081 gctccggctt gccagcggaa ctccccggac gggagcgcgt ttcttcgggt ggataccgcg
     1141 tactgccgca acgagagcgg cgatgtgctc aggagtggtg ccgcaacagc
//
LOCUS       DAOTIL010000960         1189 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminobacteriaceae bacterium isolate AMR_MDS_3026
            NODE_142019_length_1189_cov_2.626984, whole genome shotgun
            sequence.
ACCESSION   DAOTIL010000960 DAOTIL010000000
VERSION     DAOTIL010000960.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868050
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminobacteriaceae bacterium (wastewater metagenome)
  ORGANISM  Aminobacteriaceae bacterium
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae.
REFERENCE   1  (bases 1 to 1189)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1189)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:19:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,224
            CDSs (total)                      :: 3,198
            Genes (coding)                    :: 3,184
            CDSs (with protein)               :: 3,184
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1189
                     /organism="Aminobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_142019_length_1189_cov_2.626984"
                     /isolate="AMR_MDS_3026"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:3034609"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(20..319)
                     /locus_tag="P5201_14830"
     CDS             complement(20..319)
                     /locus_tag="P5201_14830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006300056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase family protein"
                     /protein_id="HRV99872.1"
                     /translation="MTQLERTVSILEANKELLKQFHVCSLSVFGSVARGEAGPDSDID
                     LLVDFEPGARIGFFAFARLQKCLEELLGTKVDLVTPDALHEGMRAEIIEESVHAV"
     gene            518..712
                     /locus_tag="P5201_14835"
     CDS             518..712
                     /locus_tag="P5201_14835"
                     /inference="COORDINATES: protein motif:HMM:NF019540.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003729 - mRNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicA family
                     toxin"
                     /protein_id="HRV99873.1"
                     /translation="MIKNKTSKEMIEIIEADGWTLDRIRGDHHQFVHTTKPGVVTIPH
                     PEKNLGSWLIGKILKQAGLK"
     gene            744..1154
                     /locus_tag="P5201_14840"
     CDS             744..1154
                     /locus_tag="P5201_14840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006302046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicB family
                     antitoxin"
                     /protein_id="HRV99874.1"
                     /translation="MAAKKDLRIYPALFHFEEEDKLFCVEYPDLPGCVTVGENMEIAL
                     RRAKEALEGFLYSSEQNGYPVAPPTPLEKMSVPAEHILVPVSVRMDILREEMANRSIT
                     KNVTIPAWLNKMAMEHKINFSSTLQEALRERLGV"
BASE COUNT          313 a          343 c          292 g          241 t
ORIGIN      
        1 tacacgaagc ttccaacttc tagacggcat gcacggactc ctcgatgatc tccgctcgca
       61 ttccttcatg cagcgcatcg ggcgtaacga ggtcaacctt cgttccgagc agttcctcaa
      121 ggcacttctg aaggcgggcg aaggcgaaga acccaatccg ggcgccgggt tcaaagtcca
      181 ccagcaggtc gatgtcgctg tccggcccgg cctctccgcg tgcgaccgaa ccaaacaccg
      241 ataatgagca aacatggaac tgcttcaaca gttccttatt cgcttcaagg atgctcaccg
      301 tccgttcaag ctgcgtcatg gcaattcgcc tccttatcgg aaagtatatc atcgttactc
      361 cgccgccata cacacaaaga aaatgatcgt cttcgaggcg gcacgggagg aaccacgcct
      421 ccatgtggat tgcacttctc tatcggctaa catgcaaccc ccttttgaca cctataataa
      481 taagtgttac aatcacctcg gatgagaggg ggcggaaatg atcaaaaaca aaacctcgaa
      541 agagatgatt gagatcatcg aagctgacgg atggactctg gaccgcatca gaggagatca
      601 ccatcagttt gttcacacaa cgaaacccgg agttgtcacc atcccgcatc cggagaagaa
      661 cctcggaagc tggctgatcg gcaaaattct caaacaagcg ggactgaaat aagcctgctt
      721 cacccgcaaa ggagtgatct gcaatggcgg caaaaaagga tctgcgcatc tatccggctc
      781 tcttccactt tgaagaagag gacaagcttt tctgcgtcga ataccccgac ctcccgggat
      841 gcgtcacagt cggggagaac atggaaatag ccctccgcag ggcgaaggaa gcgctcgagg
      901 ggtttcttta ctcttccgaa cagaacggct accctgtcgc tccacctact cctcttgaga
      961 agatgagcgt accggccgaa catattcttg ttcccgtttc ggttcgtatg gacattctac
     1021 gcgaagagat ggccaacaga agcattacga aaaacgtcac cattcccgca tggctgaaca
     1081 agatggccat ggagcacaag atcaatttct ccagtacgct tcaggaggcc cttcgcgagc
     1141 gcctcggggt atgaacgcgc attgccccgg atcggaccgc cgaaacaca
//
LOCUS       DAOTIL010000961         1189 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminobacteriaceae bacterium isolate AMR_MDS_3026
            NODE_142039_length_1189_cov_2.382716, whole genome shotgun
            sequence.
ACCESSION   DAOTIL010000961 DAOTIL010000000
VERSION     DAOTIL010000961.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868050
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminobacteriaceae bacterium (wastewater metagenome)
  ORGANISM  Aminobacteriaceae bacterium
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae.
REFERENCE   1  (bases 1 to 1189)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1189)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:19:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,224
            CDSs (total)                      :: 3,198
            Genes (coding)                    :: 3,184
            CDSs (with protein)               :: 3,184
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1189
                     /organism="Aminobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_142039_length_1189_cov_2.382716"
                     /isolate="AMR_MDS_3026"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:3034609"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..382)
                     /locus_tag="P5201_14845"
     CDS             complement(<1..382)
                     /locus_tag="P5201_14845"
                     /inference="COORDINATES: protein motif:HMM:NF025001.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P-loop NTPase"
                     /protein_id="HRV99875.1"
                     /translation="MHEGAAIYDQAGELRRIVQTRRKNIKGLSGLRSIAILSGKGGVG
                     KSNVAVNLAMALAASGKRVVLLDADLGMANVDLLCGISPKYTLAHLVKGQRSVDEVLV
                     SLGNGVMLLPGGPGVQELAELDDDA"
     gene            complement(375..>1189)
                     /locus_tag="P5201_14850"
     CDS             complement(375..>1189)
                     /locus_tag="P5201_14850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006300472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="flagellar biosynthesis protein FlhF"
                     /protein_id="HRV99876.1"
                     /translation="KSDCRTQFNVRLEKKQDTDLLLRLIAEDVSEEHARKLVRDYEAE
                     APSKDFSEWLAGRIPVAADTNWDALGGGRVMLIGPTGVGKTTTIAKLAAIQSLWEKKN
                     VVFLTADTYRIAAPEQLRTYAKILCVPIDVVYDANEIPDIVDRYRKADLLLLDTAGRS
                     QKDSRRVEEIRVLYEAFRPDAVHLVLPANMKYRDMLDVIQRMGVVPLSYVLFTKLDET
                     TTYGALLNILLDFDKPVSFFTTGQNVPNDIEVAKGSRLAELLLRGKGDVLRA"
BASE COUNT          238 a          371 c          331 g          249 t
ORIGIN      
        1 gggcgtcgtc atcaagttcc gcaagctcct gcactcccgg cccgcccggg aggagcatta
       61 ccccgtttcc cagggagaca agcacctcgt ccacgcttcg ctgccccttc accaggtgcg
      121 caagagtgta cttcggcgag attccacaga gaagatcgac attcgccatc ccgagatccg
      181 cgtcgagaag gacgacgcgc ttccctgaag cagcgagcgc catggcgaga ttcaccgcga
      241 cgttgctctt tcctaccccg ccttttccac tgagaatggc gatggaacgg aggcccgaca
      301 gccccttgat gttcttacgg cgtgtttgta caattctgcg aagttctccc gcctgatcat
      361 agatcgccgc accctcatgc acgaagaaca tctcccttcc cgcgaaggag gagttcggca
      421 aggcgtgagc ccttggccac ttcaatatcg ttcgggacgt tctgtcctgt ggtgaagaaa
      481 gagaccggtt tgtcgaagtc gagcagaata ttcagcaggg cgccgtaggt cgttgtctcg
      541 tcaagcttcg tgaaaagaac gtacgaaagc ggaacaacgc ccatccgttg gatgacgtcg
      601 agcatgtcgc ggtacttcat gttcgcggga agcaccaggt ggaccgcatc cggacggaat
      661 gcctcgtaga gcacccgaat ctcctcaacg cgacggctat ccttctggga gcggccggcg
      721 gtgtccagca gcagaagatc cgccttcctg tagcgatcga cgatgtccgg aatttcgttc
      781 gcgtcgtaca ccacatcgat cggcacgcag agaatcttcg catacgttcg cagctgctcc
      841 ggggcggcga tccggtacgt atccgcagtg agaaaaacga cgttcttctt ctcccagagc
      901 gactgaatcg ccgcgagctt cgcgatcgtc gtggtcttcc cgactccggt gggaccgatg
      961 agcatcaccc ggcctccgcc gagagcgtcc cagttcgtgt ccgcagcgac gggaattctc
     1021 ccggccagcc attcagagaa gtcctttgaa ggagcctcgg cttcgtagtc ccgaacaagc
     1081 ttccgggcgt gttcttcgct cacatcctcc gcgatcagac gcaagagcag gtcggtatcc
     1141 tgttttttct ccagccttac gttgaactga gtccggcagt cggacttct
//
LOCUS       DAOTIL010000962         1186 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminobacteriaceae bacterium isolate AMR_MDS_3026
            NODE_142551_length_1186_cov_3.119363, whole genome shotgun
            sequence.
ACCESSION   DAOTIL010000962 DAOTIL010000000
VERSION     DAOTIL010000962.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868050
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminobacteriaceae bacterium (wastewater metagenome)
  ORGANISM  Aminobacteriaceae bacterium
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae.
REFERENCE   1  (bases 1 to 1186)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1186)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:19:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,224
            CDSs (total)                      :: 3,198
            Genes (coding)                    :: 3,184
            CDSs (with protein)               :: 3,184
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1186
                     /organism="Aminobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_142551_length_1186_cov_3.119363"
                     /isolate="AMR_MDS_3026"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:3034609"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            41..964
                     /locus_tag="P5201_14855"
     CDS             41..964
                     /locus_tag="P5201_14855"
                     /EC_number="1.3.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009201343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004152 - dihydroorotate dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate dehydrogenase"
                     /protein_id="HRV99877.1"
                     /translation="MSSRTEMHVSLGGLNLESPVIIASGVWPYDAELWSPERLEGVGA
                     LCTKAVSLNGRAGNKGVRVWETPCGMLNSIGLQNAGVRSFLDDYLPLVRGANRPFLVN
                     LVMESEREVRESIAALAEVPGIPAIELNISCPNVDGDGMAWGISAESAAKAVRIARSE
                     WKGALWVKLTPQSPDLAGVAKAVEAEGADAIVCGNTWLGMAMDIKKKRPAFQRVVAGL
                     SGPAVFPLALRTVWQVCGAVKIPVVGCGGVASWEDATEMLLAGAAAVEVGAALFTDFD
                     LPRKIVAGLSAYLAEQKIDSVSGLTGLGRHE"
     gene            981..>1186
                     /locus_tag="P5201_14860"
     CDS             981..>1186
                     /locus_tag="P5201_14860"
                     /EC_number="4.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013048976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004590 - orotidine-5'-phosphate
                     decarboxylase activity [Evidence IEA];
                     GO_process: GO:0006207 - 'de novo' pyrimidine nucleobase
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="orotidine 5'-phosphate decarboxylase"
                     /protein_id="HRV99878.1"
                     /translation="MNNGLDRLILALDVERVEEGRELLASLKGELKYVKIGHQLYARG
                     GFPFLREIGAMGFSIFLDLKLHDIP"
BASE COUNT          196 a          364 c          422 g          204 t
ORIGIN      
        1 gtggacggcc cggtcttcac tgcggaggag gtgctgtgga atgagttcgc ggactgagat
       61 gcacgtttcg ctcggcggac tgaacctcga atcgccggtg atcatcgcgt ccggagtctg
      121 gccgtacgat gcggaactct ggtcgccgga acggctggaa ggcgtcggcg cgctctgcac
      181 caaggccgtc agcctgaacg gacgggcggg gaacaagggc gtccgcgtct gggagacgcc
      241 ctgcggcatg ctgaacagca tcggcctgca gaacgccggg gtgcgttcct tccttgacga
      301 ctacctcccc ctcgtccggg gcgcgaaccg cccctttctc gtgaatctcg tcatggagtc
      361 cgagcgcgag gtgcgggaga gcatcgccgc gcttgcggaa gtccccggca tccccgccat
      421 agagctgaac atctcctgcc ccaacgtgga cggcgacggc atggcgtggg ggatctcggc
      481 cgaaagcgcc gcgaaggcgg tgcgcatcgc gcggagcgag tggaagggcg cgctgtgggt
      541 gaagctcacc ccgcagtcgc cggacctcgc cggagtggcg aaggccgtcg aggcggaggg
      601 cgcggacgcg atcgtctgcg ggaatacgtg gctcggtatg gcgatggaca tcaagaaaaa
      661 acgccccgcc ttccagaggg tggtcgccgg gctctccggc cccgcggtct tcccgctcgc
      721 gctgcgcacg gtctggcagg tctgcggcgc ggtgaagatt cccgtggtcg ggtgcggcgg
      781 cgtcgcctcg tgggaggatg cgacggagat gctcctcgcc ggggctgcgg cggtggaggt
      841 cggcgccgcg ctcttcaccg acttcgactt gccccgaaag atcgtcgccg ggctttcagc
      901 ctaccttgct gaacagaaga tcgacagtgt ctccgggctg accggcctgg ggcgtcatga
      961 ataacaggag gatcgcgaag atgaacaacg gactggaccg gctcattctg gcgctcgacg
     1021 tcgaacgtgt ggaggagggg cgcgagcttc tcgcctcgct caagggagag ctgaagtatg
     1081 taaagatagg ccatcagctc tacgcccgcg gaggatttcc cttccttcgc gagatcggcg
     1141 cgatggggtt ctcgatcttc ctcgacctca agctccacga tattcc
//
LOCUS       DAOTIL010000963         1184 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminobacteriaceae bacterium isolate AMR_MDS_3026
            NODE_142935_length_1184_cov_2.711249, whole genome shotgun
            sequence.
ACCESSION   DAOTIL010000963 DAOTIL010000000
VERSION     DAOTIL010000963.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868050
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminobacteriaceae bacterium (wastewater metagenome)
  ORGANISM  Aminobacteriaceae bacterium
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae.
REFERENCE   1  (bases 1 to 1184)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1184)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:19:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,224
            CDSs (total)                      :: 3,198
            Genes (coding)                    :: 3,184
            CDSs (with protein)               :: 3,184
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1184
                     /organism="Aminobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_142935_length_1184_cov_2.711249"
                     /isolate="AMR_MDS_3026"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:3034609"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..445)
                     /locus_tag="P5201_14865"
     CDS             complement(<1..445)
                     /locus_tag="P5201_14865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014806169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-basal body complex protein"
                     /protein_id="HRV99879.1"
                     /translation="MLRSLMSGVSGARGHQTFLDVVGNNIANINTVGFKKSNVTFQDL
                     LYQTHRGAMPPVEGRGGVNSLQVGLGVRVAAIETIHTQGNINYVGGDTNVAIQGEGYY
                     VVMDGNNRLFTRGGNFTLDSENKIVQSGTGYRVGDHLVPLAPVPRS"
     gene            complement(562..978)
                     /locus_tag="P5201_14870"
     CDS             complement(562..978)
                     /locus_tag="P5201_14870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006300449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook capping FlgD N-terminal
                     domain-containing protein"
                     /protein_id="HRV99880.1"
                     /translation="MAIQNVTNSYSAKTTDTAVTDAIQNPSTDLGKDAFLKILITQLS
                     NQDPMDPLKDKDFIAQMAQFSTLEQMTNMNKSIEQMVSMNKASAVSYIGRVIEYTDDE
                     GLPTYATVGYVRFEEGKIILNTTDGDVPLEKVISVA"
     gene            complement(991..>1184)
                     /locus_tag="P5201_14875"
     CDS             complement(991..>1184)
                     /locus_tag="P5201_14875"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRV99881.1"
                     /translation="GVHLGEFIVDLRDNSKGQSGRNAFAGERNGRRRGVSVLDAEGID
                     ETIPSFRMDLEHGLLSWVA"
BASE COUNT          212 a          344 c          322 g          306 t
ORIGIN      
        1 gggaacgggg tacaggggcc aggggtacca gatggtcgcc gaccctgtac cccgttcccg
       61 actgcacaat cttgttctcg gagtcgaggg tgaaattgcc gccccttgtg aagagccggt
      121 tgttcccgtc catgacgaca taataccctt cgccctggat ggcgacattc gtatcgccgc
      181 ctacgtagtt gatattcccc tgcgtgtgga tcgtctcgat tgcggcgacg cggacgccaa
      241 gccccacctg cagggagttg actcctcctc ttccttccac gggcggcatg gcgccgcggt
      301 gcgtctggta gagaagatcc tggaaggtga cgttggattt cttgaatccc actgtattga
      361 tgttggcgat gttattgccg acgacgtcga gaaacgtctg gtgtcctctc gcgcccgaca
      421 cgcccgacat aagagatcga agcatgttca ttcctccttg ctgtatgttc gaagacggcg
      481 cagtccattg ctccgatcac ttcaaggccg tccatccgtg gggccgcttt tccgatattt
      541 ccgtgaatgc gaaaatgtcc tttaggccac ggagatgacc ttttccagtg gtacgtctcc
      601 gtcggtggta ttcagaatga tctttccttc ttcgaaacga acgtaaccga ccgttgcgta
      661 ggtgggaagc ccctcgtcgt ccgtatattc gatcaccctg ccgatgtagc tgacggcgga
      721 ggccttgttc atggacacca tctgttcgat gctcttgttc atgttggtca tctgctcaag
      781 cgtgctgaac tgcgccatct gggcgatgaa atccttgtcc ttcaaaggat ccatcgggtc
      841 ctggttgctc agctgcgtta tgagtatttt aagaaacgca tccttcccga gatcggtgct
      901 cggattctgg atggcgtctg tgaccgccgt atccgtcgtc tttgcggagt agctgtttgt
      961 tacgttctgt atggccattc cttccacctc ctaggctacc caggagagca gcccatgctc
     1021 aagatccatc cggaaggacg ggatcgtttc gtcaatgccc tctgcgtcca agacggagac
     1081 tcccctccgc cggccgtttc tctcgccggc gaacgcgttg cgccctgatt gtcctttgct
     1141 gttatcgcga aggtccacga tgaattcgcc gagatgaacc ccct
//
LOCUS       DAOTIL010000964         1182 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminobacteriaceae bacterium isolate AMR_MDS_3026
            NODE_143395_length_1182_cov_1.614020, whole genome shotgun
            sequence.
ACCESSION   DAOTIL010000964 DAOTIL010000000
VERSION     DAOTIL010000964.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868050
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminobacteriaceae bacterium (wastewater metagenome)
  ORGANISM  Aminobacteriaceae bacterium
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae.
REFERENCE   1  (bases 1 to 1182)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1182)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:19:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,224
            CDSs (total)                      :: 3,198
            Genes (coding)                    :: 3,184
            CDSs (with protein)               :: 3,184
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1182
                     /organism="Aminobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_143395_length_1182_cov_1.614020"
                     /isolate="AMR_MDS_3026"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:3034609"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            47..934
                     /locus_tag="P5201_14880"
     CDS             47..934
                     /locus_tag="P5201_14880"
                     /inference="COORDINATES: protein motif:HMM:TIGR01784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rpn family recombination-promoting
                     nuclease/putative transposase"
                     /protein_id="HRV99882.1"
                     /translation="MNPRVDFVFKKLFGTEESKDILIDLINSIVSEKDTVADLELRNP
                     YNEKQFLEDKRSVLDIKARSAQGKWYDIEMQVLPEDWFAKRSLYYWSRLYSEQLQEGK
                     HYRDLTKTICINILNFSQLHDEPSYHNVYKVRNVVSNSELLDEFELHFIELRKYDESL
                     ADSLKTALDRWVNFLKLAGVYDSETMPEELKASPPVRKAMNLLERLSATKEERAIYDD
                     RLKWIRDVESSLETMERRGRELGRAEGIEIGREQGLEQAKKENARKMLRKGMSIADVS
                     EYADLPEDAVRLLASEFQD"
BASE COUNT          322 a          294 c          333 g          233 t
ORIGIN      
        1 atctcgaata gatttcgtga aaaaggggga atgccgcgtg tgcaaaatga acccgagagt
       61 ggatttcgtc ttcaaaaaac tcttcggaac cgaggaaagc aaggacattc tcatcgacct
      121 tatcaactcg atagtcagcg aaaaagatac ggtcgccgac ctcgaactaa ggaatcccta
      181 caacgagaag cagtttctgg aagacaagcg gagcgtgctc gacataaaag cccggagcgc
      241 gcaggggaag tggtacgaca tcgaaatgca ggtgcttccc gaggattggt ttgcaaagcg
      301 ctccctgtac tactggagca gactgtacag cgaacagctt caggaaggga agcactatag
      361 ggacctgacg aaaacgatct gtattaatat cctgaacttc agtcagctcc atgacgagcc
      421 ttcgtaccac aacgtctata aagtgcggaa cgtcgtcagc aactccgaac tgctcgacga
      481 attcgaactc catttcatcg agcttcgcaa gtacgacgaa tccttggcgg actctctgaa
      541 gactgccctg gaccggtggg tgaacttttt gaaactcgcc ggtgtatacg attcggagac
      601 gatgccggaa gaactgaagg catcccctcc cgtcaggaag gcgatgaatc ttctagaacg
      661 tctctcggcg acgaaagagg agcgggcgat ttacgacgac cggctgaaat ggattcgcga
      721 cgtcgagagt tcgctggaga cgatggagcg aagaggtcgg gagttgggga gagccgaggg
      781 tatcgaaata ggcagagaac agggccttga gcaggcgaaa aaagaaaatg cccgtaaaat
      841 gttgcggaaa ggcatgagca ttgccgatgt ttcggagtac gcggatcttc cggaggatgc
      901 ggtacggctt ctggcatcgg agtttcaaga ttgatgcgga gattacggga aaaaagtcga
      961 aaagaaagcc gtccggagag gcaaatcact tccgggcggc ttattttatc cgaagagacg
     1021 attcacaccc gccgtaaaat ctccagcacg cgttccttct ccacgggctt ctgcaaaaag
     1081 tcgcacgcac ccgcgtcgat cgccttcttc aaattactct tcgtcccggt ggacgagagg
     1141 atcaccaccc gcgcgtccgg gtaccgctcc cgtatctcgg cc
//
LOCUS       DAOTIL010000965         1180 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminobacteriaceae bacterium isolate AMR_MDS_3026
            NODE_143651_length_1180_cov_3.293333, whole genome shotgun
            sequence.
ACCESSION   DAOTIL010000965 DAOTIL010000000
VERSION     DAOTIL010000965.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868050
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminobacteriaceae bacterium (wastewater metagenome)
  ORGANISM  Aminobacteriaceae bacterium
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae.
REFERENCE   1  (bases 1 to 1180)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1180)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:19:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,224
            CDSs (total)                      :: 3,198
            Genes (coding)                    :: 3,184
            CDSs (with protein)               :: 3,184
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1180
                     /organism="Aminobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_143651_length_1180_cov_3.293333"
                     /isolate="AMR_MDS_3026"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:3034609"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..848
                     /locus_tag="P5201_14885"
     CDS             <1..848
                     /locus_tag="P5201_14885"
                     /inference="COORDINATES: protein motif:HMM:NF018485.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HRV99883.1"
                     /translation="SGFIPMKPELIPPEKRGISSGFIGMGMALGILGGMAVSGPLYEF
                     FGHYRPPFLVLAVPTVLMLFAFVRFLPDTRNIGGSPSLSVYKRLFRDKDIWKINMATF
                     CSLYGFWAAATWGPTFLQAERGFSLSGSGLYTGLIALSALPGGVLWGRLSDRFGRRKI
                     AALVLPASGVALFLLTRVQGAPLIVSTLLLFGIFSNTAFSPVAVAWIGDIVSKRHPGS
                     MSAAVGFFNATIMSSAVIAPLVSGFLRDMTGSLVPAILTGSLLMFFGTVMLLLTPDAA
                     RAGAE"
     gene            873..>1180
                     /locus_tag="P5201_14890"
     CDS             873..>1180
                     /locus_tag="P5201_14890"
                     /inference="COORDINATES: protein motif:HMM:NF013383.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-binding domain-containing protein"
                     /protein_id="HRV99884.1"
                     /translation="MAEITVFGGGTWGTALAVSAAKSGHSVMLWCRRAEQARAINATS
                     HNPDYLQSIALPEAISSTSDPEEAALFSQYWILALPTQTVRGFLPALAPFCGKETEVC
                     "
BASE COUNT          176 a          397 c          353 g          254 t
ORIGIN      
        1 gctccggctt catcccgatg aagccggagc tgattccgcc ggagaaacgc ggaatcagct
       61 ccggcttcat cgggatgggg atggcgctcg gtattcttgg aggtatggcg gtgagcgggc
      121 cgctctacga gttcttcggt cactaccgtc ctccgttcct tgtgctcgcc gtgccgacgg
      181 tgcttatgct cttcgcattc gtccgttttc tccccgacac caggaatatc gggggatccc
      241 cgtcgctttc tgtctacaag cgtctcttcc gggacaagga catctggaag atcaacatgg
      301 cgacgttctg ctcgctctac ggcttctggg ccgccgcgac atggggaccg acgttccttc
      361 aggcggagcg ggggttttcg ctctcaggct ccgggctgta caccgggctc atcgcgctct
      421 ccgcgcttcc gggaggcgtg ctctggggca ggctctccga ccgcttcggc cgcaggaaga
      481 tcgccgcgct cgtgctgccg gcgagcggcg tggcgctctt ccttctcacc cgcgttcagg
      541 gagcgccgct catcgtttcc acgctgctgc tcttcggcat cttcagcaac acggcattct
      601 cgcccgttgc agtcgcatgg ataggcgaca tcgtgagcaa acgccaccct ggctcgatga
      661 gcgcggcggt cggttttttc aacgcgacga tcatgtcctc ggcggtgatt gcgcctctcg
      721 tctccggctt tctgcgcgac atgacgggct ccctggtacc cgccatcctc acgggctccc
      781 tgctgatgtt cttcggtacc gtaatgctgc tgctgactcc cgacgccgcc cgggcgggcg
      841 cggagtaata cacgaaagga gcaattcccg tcatggctga aatcacagta ttcggcggag
      901 gaacctgggg tacggcgctc gccgtctccg ccgccaagag cgggcactcc gtcatgctct
      961 ggtgccgccg cgccgaacag gcgcgagcga tcaacgcgac ctcgcataac ccggactatc
     1021 tccagtcgat cgctcttccc gaggcgatct cgtccacctc cgatcccgag gaagcggcgc
     1081 tcttctctca gtactggatc ctcgcgctgc caacgcaaac ggttcggggt ttccttccgg
     1141 cgctcgcccc tttctgcggg aaagagaccg aagtctgcaa
//
LOCUS       DAOTIL010000966         1178 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminobacteriaceae bacterium isolate AMR_MDS_3026
            NODE_144010_length_1178_cov_4.356189, whole genome shotgun
            sequence.
ACCESSION   DAOTIL010000966 DAOTIL010000000
VERSION     DAOTIL010000966.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868050
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminobacteriaceae bacterium (wastewater metagenome)
  ORGANISM  Aminobacteriaceae bacterium
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae.
REFERENCE   1  (bases 1 to 1178)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1178)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:19:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,224
            CDSs (total)                      :: 3,198
            Genes (coding)                    :: 3,184
            CDSs (with protein)               :: 3,184
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1178
                     /organism="Aminobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_144010_length_1178_cov_4.356189"
                     /isolate="AMR_MDS_3026"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:3034609"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..589)
                     /locus_tag="P5201_14895"
     CDS             complement(<1..589)
                     /locus_tag="P5201_14895"
                     /inference="COORDINATES: protein motif:HMM:TIGR00654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PhzF family phenazine biosynthesis isomerase"
                     /protein_id="HRV99885.1"
                     /translation="MKTYKYYKLNAFVKGNSEGNPAAFLDLGDETLSDESMQRIAKEH
                     AGFVSEVVYARKNEEREIFLTYYSSECEVAFCGHGTIATMYEIIRRDEALRSAKVIEI
                     CTNKKGRLNIYNRINEEDCIYISAPDPVMLGCTLSRASIAEALGIREEAISDTLPVDI
                     IDAGLRTLLVPIRALEQEVSMYPDEQALKSYCLRND"
     gene            complement(586..>1178)
                     /locus_tag="P5201_14900"
     CDS             complement(586..>1178)
                     /locus_tag="P5201_14900"
                     /inference="COORDINATES: protein motif:HMM:NF024109.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HRV99886.1"
                     /translation="LRNQVLAFDLPEHGERKGENYPCTVQNGVHDLRTVYDSVKDSYR
                     SVSLFACSLGAYFSLVAYRDVVFDKCLFLSPLLDMERLIRNMMHWANVDETQLQKERE
                     IPTSFGETLSWDYYEYVRAHPVDRWDSPTSILYGENDNLTERCVLDAFAQKFACGVTV
                     MSGGEHYFHTPEQLAFLKNWIREQNGDGGISKEGKR"
BASE COUNT          221 a          339 c          336 g          282 t
ORIGIN      
        1 cgtcgttgcg caggcagtag gatttcagag cttgctcgtc ggggtacatc gacacctcct
       61 gctccagagc gcgtatcggc acaagcagcg tccgaagccc cgcgtcgatg atatctacgg
      121 gcagcgtatc cgaaatggcc tcttcacgga tgcccagggc ttcggcgatc gacgcccgcg
      181 agagggtgca tccgagcatc accggatccg gcgcggagat atagatgcag tcctcttcgt
      241 tgatgcggtt gtagatgttc agcctgccct tcttatttgt gcaaatttcg atgaccttcg
      301 cgcttctcag ggcctcgtcc cgccggatga tctcgtacat ggtcgctatc gttccgtgtc
      361 cgcagaaggc gacttcgcac tccgaggaat agtaggtcag aaaaatctcc cgctcctcgt
      421 tttttcttgc gtacaccact tccgacacga agcccgcatg ctccttcgcg atgcgctgca
      481 tgctctcgtc ggagagcgtt tcgtcgccga ggtcgagaaa agcggccgga tttccttcgg
      541 agttcccctt tacgaaggcg ttgagcttgt agtatttata cgttttcatc tctttccctc
      601 cttggaaatg cctccgtcgc cgttctgctc acgtatccag ttcttcagga aagcgagctg
      661 ctcgggagtg tggaagtagt gctcgccgcc gctcatgacc gttactccgc aggcgaattt
      721 ttgcgcgaag gcgtcaagaa cgcatcgttc cgtcagattg tcgttctccc cgtagagtat
      781 cgaggtcggg ctgtcccagc ggtcgaccgg gtgcgcccga acatattcat agtagtccca
      841 ggagagcgtc tcgccgaagg atgtcggtat ctcacgttcc ttttgcaact gcgtttcatc
      901 cacattcgcc cagtgcatca tgttccggat aaggcgttcc atgtcgagca gcggcgagag
      961 gaagaggcac ttgtcgaaaa cgacgtctct gtaggcgacc aggctgaagt aggcgccgag
     1021 gctgcatgcg aacaaagaga cgctgcggta cgagtctttt acggaatcgt acacggttcg
     1081 caggtcatgg acgccgttct gaacggtgca ggggtagttt tcccccttcc gctcgccgtg
     1141 ctccggcagg tcgaaggcaa ggacctgatt tcgcagga
//
LOCUS       DAOTIL010000967         1178 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminobacteriaceae bacterium isolate AMR_MDS_3026
            NODE_144023_length_1178_cov_3.703473, whole genome shotgun
            sequence.
ACCESSION   DAOTIL010000967 DAOTIL010000000
VERSION     DAOTIL010000967.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868050
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminobacteriaceae bacterium (wastewater metagenome)
  ORGANISM  Aminobacteriaceae bacterium
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae.
REFERENCE   1  (bases 1 to 1178)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1178)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:19:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,224
            CDSs (total)                      :: 3,198
            Genes (coding)                    :: 3,184
            CDSs (with protein)               :: 3,184
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1178
                     /organism="Aminobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_144023_length_1178_cov_3.703473"
                     /isolate="AMR_MDS_3026"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:3034609"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            269..892
                     /locus_tag="P5201_14905"
     CDS             269..892
                     /locus_tag="P5201_14905"
                     /inference="COORDINATES: protein motif:HMM:NF012655.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HRV99887.1"
                     /translation="MNLVKKPKTARGAETLQRICDAAEELFAESGYYNTQISDIAKKA
                     GIASGTFYIYFPDKISAFRYLLDELGRRLRREIRAAASGARTQQEAEFVGLKTFLSFA
                     SKHIGLFKIVWQAQFVDMEAFKNYYESFAARYVTRIGDAQKRGELTEMDPEYFSYILM
                     GIYNFVALKCIVFDGKEPTDELVEEVFKFIRHGAFAPSAENGAPRPQ"
     gene            complement(969..>1178)
                     /locus_tag="P5201_14910"
     CDS             complement(969..>1178)
                     /locus_tag="P5201_14910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_084002417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine/sarcosine/betaine reductase complex
                     selenoprotein A"
                     /protein_id="HRV99888.1"
                     /translation="SNGDPTYAGPLAGVSLGLPVYHIFDGDIRNECDPAAWEEQISMM
                     EMVLDPDALAAAVKGIRDQFSTHSL"
BASE COUNT          253 a          347 c          364 g          214 t
ORIGIN      
        1 caaaggcaga gtgctcctgg agtccgtcgg cagttcaaaa tgaagtgcgc gagaagttgt
       61 gaaaaaggag accgattgag aacaagagca acgcaactga cggaaggaaa cggaggcccc
      121 gcccgacgag aacgttcgca ggcgtgcgtc tcctcctctc cggagaacag agctcactac
      181 tctctctact cgcttccccg ggacccgccc tcccgcccct tctgtgctat catgtgaggc
      241 ggagtaattc gtcgaacggg gtggtagcgt gaatctggtc aaaaaaccga agacggcacg
      301 gggggcggag acgctgcagc gcatctgcga cgcggcggag gagctgttcg cggagagcgg
      361 gtactacaac acgcagataa gcgacatcgc gaaaaaagcg gggatcgcct ccgggacgtt
      421 ctatatctac tttcccgaca agatcagcgc attccgctac ctgctggacg aactgggccg
      481 ccgcctcagg cgggagatac gcgccgccgc ttccggggcg cggacgcagc aggaggccga
      541 gttcgtcggg ctcaagacgt tcctctcgtt cgcttcgaag cacatcgggc tcttcaagat
      601 cgtctggcag gcgcagttcg tcgatatgga agcgttcaag aactactacg agagcttcgc
      661 cgcccgctac gtcacgcgca tcggcgacgc gcagaagcgc ggcgaattga cggagatgga
      721 cccggagtac ttctcctaca tcctgatggg aatctacaac ttcgtggccc tgaagtgcat
      781 cgtcttcgac ggaaaggagc cgacggacga gcttgtggag gaggtcttca agttcatcag
      841 gcacggtgcg ttcgcgccgt ccgcggagaa cggagctccc cggccgcagt gagccggaca
      901 tgctcgcgca tatttcaacg aactaaacac ggcccccgcg atttctcgcc gggggccgct
      961 tcatactttt acaacgagtg tgtgctgaac tggtcccgaa tgccttttac ggccgccgca
     1021 agggcgtcgg ggtcgagaac catctccatc atgctgatct gctcttccca ggcggcagga
     1081 tcgcattcat tgcgaatgtc gccgtcgaag atatggtaca cggggagtcc caacgagact
     1141 cccgcgagag ggcctgcata ggtcggatcg ccgttgct
//
LOCUS       DAOTIL010000968         1178 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminobacteriaceae bacterium isolate AMR_MDS_3026
            NODE_144173_length_1178_cov_1.431879, whole genome shotgun
            sequence.
ACCESSION   DAOTIL010000968 DAOTIL010000000
VERSION     DAOTIL010000968.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868050
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminobacteriaceae bacterium (wastewater metagenome)
  ORGANISM  Aminobacteriaceae bacterium
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae.
REFERENCE   1  (bases 1 to 1178)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1178)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:19:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,224
            CDSs (total)                      :: 3,198
            Genes (coding)                    :: 3,184
            CDSs (with protein)               :: 3,184
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1178
                     /organism="Aminobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_144173_length_1178_cov_1.431879"
                     /isolate="AMR_MDS_3026"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:3034609"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..947
                     /locus_tag="P5201_14915"
     CDS             <1..947
                     /locus_tag="P5201_14915"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRV99889.1"
                     /translation="RTIIKEALIKRDLQRGRITLFDWTALRFPDEWIFGIAGETRGMG
                     KKREVLDARFMIVKPDGAFEMKRVPARIGLEDEYQRYILTMQEALATDWTKDRVFEGL
                     VARNGSVNCIYRSEEITLPDLDGIARILDETGDELPGGKKTGSELAALLVEFQKGIAL
                     PADGKHSGRIESLKRALLERDTETITKKELRRNINEHLGKTGMLPQAFRRYLLDEHGI
                     RLVFPRSRESVERLLPALCEIKYFEEGKNRACYFVGEKGQGIKRTVKSACHIREVEAV
                     EGSDLLFRDILPTMDVDFVRTGQSTVVPFVFKYLRERK"
     gene            958..>1178
                     /locus_tag="P5201_14920"
     CDS             958..>1178
                     /locus_tag="P5201_14920"
                     /inference="COORDINATES: protein motif:HMM:NF012372.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA cytosine methyltransferase"
                     /protein_id="HRV99890.1"
                     /translation="MLGLVSRAGGNNNTIKTIELFCGIGGFRLACDQLFFRTVWANDI
                     NHLSCRVYADAFGTTGLYEGDIRELHSTIP"
BASE COUNT          284 a          296 c          357 g          241 t
ORIGIN      
        1 tgcggacgat catcaaggag gccctgatca aaagggatct gcaacgcggc cgcatcaccc
       61 tcttcgactg gacggcgctc cgcttcccgg acgagtggat cttcgggatc gcgggagaga
      121 cgcggggaat ggggaaaaaa cgcgaagtgc tcgacgcgcg cttcatgatc gtcaaaccgg
      181 acggcgcctt cgagatgaag cgcgttcccg cccggatcgg actggaggac gagtaccagc
      241 gctacatcct gacgatgcag gaagcccttg ccacggactg gacgaaagac cgcgtgttcg
      301 aggggctcgt cgcccgcaac ggcagcgtca actgcatcta ccgctccgaa gagatcaccc
      361 tccccgacct cgacggcatc gcccgcattc tggacgaaac aggcgacgaa ctacccgggg
      421 ggaagaagac ggggagcgaa ctcgccgctc ttctggtaga atttcaaaag ggcatcgccc
      481 ttcctgcgga cgggaagcac tccggaagga ttgaaagcct gaaaagagcc cttctggagc
      541 gggacacgga gacaataacc aagaaagagc ttcgccggaa catcaacgaa catctgggaa
      601 agacgggtat gcttccccaa gccttccgcc gctatctcct cgacgaacac ggcatccgcc
      661 tcgtcttccc gcggagcagg gagagcgtgg aaaggttgtt accggccctg tgcgagatca
      721 aatacttcga ggagggcaaa aacagagcgt gctacttcgt cggagagaag gggcagggga
      781 taaagcggac ggtcaagagc gcctgccaca tccgtgaagt ggaggccgtt gaaggatccg
      841 atttgctttt ccgtgacatt ctgcctacaa tggacgttga ttttgtacgg acggggcaga
      901 gtacggtagt gccgtttgtg tttaagtatt tgagggagag aaagtaggta tggtagtatg
      961 cttggtttag tatcgagggc gggtggaaat aataatacta ttaaaaccat tgagcttttt
     1021 tgtggtattg gcggtttccg tctggcctgc gatcagttat tttttcgtac ggtttgggca
     1081 aacgatataa atcacctatc atgtcgtgtg tatgctgatg cttttggaac tacaggctta
     1141 tatgaggggg atattcgaga gctacactcc actatacc
//
LOCUS       DAOTIL010000969         1176 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminobacteriaceae bacterium isolate AMR_MDS_3026
            NODE_144370_length_1176_cov_5.773417, whole genome shotgun
            sequence.
ACCESSION   DAOTIL010000969 DAOTIL010000000
VERSION     DAOTIL010000969.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868050
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminobacteriaceae bacterium (wastewater metagenome)
  ORGANISM  Aminobacteriaceae bacterium
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae.
REFERENCE   1  (bases 1 to 1176)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1176)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:19:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,224
            CDSs (total)                      :: 3,198
            Genes (coding)                    :: 3,184
            CDSs (with protein)               :: 3,184
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1176
                     /organism="Aminobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_144370_length_1176_cov_5.773417"
                     /isolate="AMR_MDS_3026"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:3034609"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>1176
                     /locus_tag="P5201_14925"
     CDS             <1..>1176
                     /locus_tag="P5201_14925"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRV99891.1"
                     /translation="TAMTKAPERFGVIVKDEKALTSVGDFPVIETKALSIEEICAARN
                     EFFEGTTGVMRKMFRDGGIPDETVMRSYALSERYGLSGMWQSLAYSKFPYLDAQYKAR
                     IRGDALTRDYDMHSMFDTYPQRVFLLSLTPSLAADIPSVNGFVVSPFEFEVLKHSSGK
                     LTMLEMAEAIFPDCRSAFENFEDFKPHLLETVKKLEAAGMCVLSGPLNRKAAERKWKK
                     KKDAGEPRMKNKVILFKVFTIAMETAGFSRNGAFLGLYGLVSYLDEQGYDAVACECRP
                     DQVIEYIQRLPLDEVCCVGFSVDFENKHAVEKISASIAETSGIPVLIGGPEAFSLDEE
                     YLRGSKAAAIVRGEGELAMTAVLEALKAKKSLDAIPGILYIDENGECVDRGEGPVVE"
BASE COUNT          287 a          289 c          359 g          241 t
ORIGIN      
        1 aacggcgatg acgaaagccc ccgaaaggtt cggcgttatt gtaaaagacg aaaaggcgct
       61 tacctcggtg ggggattttc cggtgatcga gacgaaagcc ctgagcatcg aagagatctg
      121 cgcggccagg aacgagtttt tcgagggaac gaccggagtc atgcgaaaga tgttccgcga
      181 cggcgggatc cccgacgaaa cggtgatgcg aagctacgcg ctgagcgaac gctacgggct
      241 cagcgggatg tggcagagcc tggcgtactc caaatttccc tacctcgacg cgcagtacaa
      301 ggccaggatt cggggggacg cgctcacaag ggactatgac atgcactcca tgttcgacac
      361 gtacccgcag cgggttttcc tgctttcgct gacgccctct ctggcggcgg acataccgtc
      421 cgtgaacgga ttcgtcgttt caccctttga atttgaagta ttgaaacact cctccggtaa
      481 gctgaccatg ctcgaaatgg cggaagcgat ttttcccgac tgtcggagcg ccttcgagaa
      541 ttttgaagat ttcaagccgc atctgctgga gaccgtcaaa aaactggaag cggcggggat
      601 gtgcgttctt tcgggcccgt tgaaccggaa agcggcggaa cgaaaatgga aaaagaaaaa
      661 agatgcaggg gagcctcgga tgaaaaacaa ggtcattctg ttcaaagtat tcaccattgc
      721 catggagaca gccggcttca gccgaaacgg agcttttctt ggactctacg ggttggtaag
      781 ttatctggat gaacaagggt atgacgccgt tgcctgcgaa tgtcgtccgg accaggtcat
      841 cgagtatatt cagcgccttc cgctcgatga agtatgctgc gtcggttttt cggtagactt
      901 cgagaacaag catgcggtcg aaaaaatcag tgcatccata gcggaaacct ccgggattcc
      961 ggtgctgatc ggaggccccg aagccttttc attggatgaa gagtatctcc gcggttccaa
     1021 agccgccgcc atcgtacggg gcgaaggcga actggccatg acggcggttt tggaggcctt
     1081 gaaagcaaag aagagcctgg atgcgattcc tggcatcttg tatatcgacg aaaacggcga
     1141 gtgcgtcgac aggggcgaag ggccggtggt ggaaaa
//
LOCUS       DAOTIL010000970         1176 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminobacteriaceae bacterium isolate AMR_MDS_3026
            NODE_144444_length_1176_cov_2.461195, whole genome shotgun
            sequence.
ACCESSION   DAOTIL010000970 DAOTIL010000000
VERSION     DAOTIL010000970.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868050
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminobacteriaceae bacterium (wastewater metagenome)
  ORGANISM  Aminobacteriaceae bacterium
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae.
REFERENCE   1  (bases 1 to 1176)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1176)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:19:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,224
            CDSs (total)                      :: 3,198
            Genes (coding)                    :: 3,184
            CDSs (with protein)               :: 3,184
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1176
                     /organism="Aminobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_144444_length_1176_cov_2.461195"
                     /isolate="AMR_MDS_3026"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:3034609"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>1176
                     /locus_tag="P5201_14930"
     CDS             <1..>1176
                     /locus_tag="P5201_14930"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRV99892.1"
                     /translation="YSGEASLFVLLALLAFLGLALLGGLFISSFVGVRDERARLAREG
                     KKRYHLRDGHAVILGWNKTAPGIVKHLRPEGGSEPMIVILSSAEAEDVRLDLAKANAY
                     KCGNIVVYHGNRADMDELECLEFEKVSRVCIVGEEDSSSSDSGTLQTVVNLLRTTKDI
                     ADGRSIPCSLHIDDPYTCALLRKVDFFDRDEREHVDIRLCNFYEGWAKRLWSSFPGCA
                     KEFPALARDPERCAKGDGVHLLIAGFGRMGQALAIEAARICHYANGAKTKITVIDKDL
                     GDLKESFVSRFFVERQGEGRFHDVEFVFVESCVESEASRRLLADAAFDPRYVPTFAIC
                     FSAMDASLTAALSLPLEVGLSGAPVLVRQQRHSGLEALAKRLSESENTERMRLWRNF"
BASE COUNT          230 a          309 c          387 g          250 t
ORIGIN      
        1 cgtattccgg cgaggcgagt ctcttcgttc ttctggccct gctggcattt ctcggacttg
       61 cactgcttgg aggactcttc atctcaagtt tcgtcggggt gcgggacgag agggcccggc
      121 tcgcccggga gggcaaaaag aggtaccacc tacgcgatgg ccacgcggtc attcttggat
      181 ggaacaagac cgcgccgggt atcgtgaagc atcttcgtcc ggaggggggg agcgagccca
      241 tgatcgtcat tctttcctcc gcggaggccg aggacgttag actggatctg gcgaaggcga
      301 acgcgtacaa gtgcggcaac atcgtcgtct atcacggtaa cagggcggat atggacgagc
      361 tggagtgttt ggagttcgag aaggtctcgc gggtctgcat cgtcggagaa gaggattcat
      421 cttcatcgga cagcggaact cttcagaccg tggtcaatct gctccgtacc acaaaagaca
      481 tcgcggacgg gcgaagcatt ccgtgttccc ttcatataga cgacccgtac acatgcgctc
      541 tccttcggaa agtcgacttc ttcgaccggg acgagagaga gcacgtggac atcaggttgt
      601 gcaacttcta cgaggggtgg gcgaaaagac tgtggagttc cttcccggga tgcgcgaagg
      661 agtttccggc cttggcgcgg gatccggaac gatgcgcgaa aggggacggc gtccatttgc
      721 tgatcgctgg cttcggacgg atggggcagg cgcttgcgat agaagcggcg cggatttgcc
      781 actatgcgaa cggggcgaaa acaaagatca ccgtgatcga caaagacctg ggtgacctga
      841 aagagagttt cgtctcccga tttttcgtgg agaggcaagg cgaaggacgt tttcacgatg
      901 tcgagttcgt gtttgtggag tcgtgcgtcg agtcggaggc gtcgcgtcgg ctgctcgccg
      961 atgcagcttt cgatccgaga tacgttccta cgttcgcgat ctgcttctcc gccatggacg
     1021 cctcgcttac cgccgcgctc tcccttcctc tggaggttgg cttatcagga gcccccgtcc
     1081 tggtgcggca gcagcggcac tccgggctgg aggctctggc aaagcgcctt tcggagagcg
     1141 agaacacgga aaggatgcgt ttgtggagga acttca
//
LOCUS       DAOTIL010000971         1174 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminobacteriaceae bacterium isolate AMR_MDS_3026
            NODE_144791_length_1174_cov_2.899017, whole genome shotgun
            sequence.
ACCESSION   DAOTIL010000971 DAOTIL010000000
VERSION     DAOTIL010000971.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868050
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminobacteriaceae bacterium (wastewater metagenome)
  ORGANISM  Aminobacteriaceae bacterium
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae.
REFERENCE   1  (bases 1 to 1174)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1174)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:19:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,224
            CDSs (total)                      :: 3,198
            Genes (coding)                    :: 3,184
            CDSs (with protein)               :: 3,184
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1174
                     /organism="Aminobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_144791_length_1174_cov_2.899017"
                     /isolate="AMR_MDS_3026"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:3034609"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>1174
                     /locus_tag="P5201_14935"
     CDS             <1..>1174
                     /locus_tag="P5201_14935"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF014425.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HRV99893.1"
                     /translation="LGSVLATWILARRLYGFPAAFFSSLILATSLLHFAIGRINIIDM
                     PLSFFLTAAIVGLWFGIDETTEEETAKNRRYLLVFYAGMALAVLSKGLIGIVLPGGIL
                     FWYILFTRRWSLVKRVLYIPGILLFFALTVPWFVEVCRRNSDFFYFFFIQEHFLRYAT
                     TMHGRYAPMWFFIPILIVGFFPWAGLLPGAIRSALPLPLRTRGREKRNDLFLILWFGV
                     IFVFFSISSSKLIPYIVPAAPPLAILMGRYLSRCIEEEDVKKTGRFLLWNSLLLFPFI
                     GALLVYPFFNDRIAAGRLLPYSLPIAAVLILFVFSGWHSFLRRDFRRLALFLCVLSFA
                     NMFAFSRVFLLYDSLLTARELAGRIAEIRNDGDIVVQFANYDQGLPFYLKQRIVLVN"
BASE COUNT          180 a          387 c          300 g          307 t
ORIGIN      
        1 ctcggcagcg tcctcgcaac gtggattctg gcgcggcgtc tgtacggttt cccggcggca
       61 ttcttctctt cgctcatcct ggcgaccagc ctgctccact tcgccatcgg gcgaatcaat
      121 atcatcgaca tgccgctctc gttcttcctg actgccgcga tagtcggcct ctggttcgga
      181 atcgatgaga cgacggaaga ggaaacggcg aaaaacagga ggtatctcct ggtcttttac
      241 gcgggaatgg cgctcgccgt cctctcgaaa ggactcatcg gcatcgtcct cccgggcggc
      301 atcctcttct ggtatattct gttcacccgt cgctggagcc ttgtcaagag ggtgctctac
      361 attcccggca tcctcctttt cttcgcgctc accgtcccct ggttcgtcga ggtctgccgc
      421 agaaacagcg acttcttcta cttctttttc atccaggaac acttcctgcg ctacgcgacg
      481 acgatgcacg gaaggtacgc gcccatgtgg tttttcatcc ccattctgat cgtcggtttc
      541 tttccctggg cggggctgct ccccggcgcg attcgctcgg ctttgcctct gcccctgcga
      601 acccgcggcc gcgaaaagcg gaacgacctc tttctcatcc tctggttcgg cgtgatcttc
      661 gtcttcttct ctatttcgag ttcgaagctg attccgtata tcgtccccgc agcgccgccg
      721 ctcgcgattc ttatggggcg gtatctctcc cgctgcatcg aggaggagga cgtcaagaag
      781 accggacgct tcctgctgtg gaattcgctg ttgctgtttc cctttatcgg cgcgctgctt
      841 gtctatccgt tcttcaacga ccggatcgcc gccggacgtc tgctccccta ctcgctcccg
      901 atcgcagcgg tcctgatcct gtttgtcttc tccgggtggc acagcttcct ccgcagggat
      961 ttcaggagac ttgccctctt cctctgcgtg ctctccttcg cgaacatgtt cgccttcagc
     1021 agggtcttcc ttctctacga ttccttgctt actgcgcgcg aactcgcggg aagaatcgcc
     1081 gaaatccgaa acgacggcga catcgtcgtc cagttcgcaa actacgacca ggggctcccc
     1141 ttctatctga aacagcggat cgtgctggtc aact
//
LOCUS       DAOTIL010000972         1173 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminobacteriaceae bacterium isolate AMR_MDS_3026
            NODE_144956_length_1173_cov_4.227191, whole genome shotgun
            sequence.
ACCESSION   DAOTIL010000972 DAOTIL010000000
VERSION     DAOTIL010000972.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868050
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminobacteriaceae bacterium (wastewater metagenome)
  ORGANISM  Aminobacteriaceae bacterium
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae.
REFERENCE   1  (bases 1 to 1173)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1173)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:19:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,224
            CDSs (total)                      :: 3,198
            Genes (coding)                    :: 3,184
            CDSs (with protein)               :: 3,184
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1173
                     /organism="Aminobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_144956_length_1173_cov_4.227191"
                     /isolate="AMR_MDS_3026"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:3034609"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..421
                     /locus_tag="P5201_14940"
     CDS             <1..421
                     /locus_tag="P5201_14940"
                     /inference="COORDINATES: protein motif:HMM:TIGR04564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="SYNERG-CTERM sorting domain-containing protein"
                     /protein_id="HRV99894.1"
                     /translation="VVEYTASWPENARYYALFRKADGSGYEIPERGIQFEIKAGQSLP
                     DGATPPDFYEEGEGLMTWRNVVTDGGSFDLEPAPGIVTFRVCSVRAAEAPAASSGGSG
                     CSTDVASPLALLLAIPLLLLRKMQRKTHRTSQGGDQR"
     gene            418..573
                     /locus_tag="P5201_14945"
     CDS             418..573
                     /locus_tag="P5201_14945"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRV99895.1"
                     /translation="MTQERKKAEAKLEDNLKPEELKKKKKLGEQDDAELDGISGGEGM
                     DTGKIIP"
     gene            729..>1173
                     /locus_tag="P5201_14950"
     CDS             729..>1173
                     /locus_tag="P5201_14950"
                     /inference="COORDINATES: protein motif:HMM:NF024874.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HRV99896.1"
                     /translation="MELQLIRNATVKMSFAGKMLLLDPYLGEKGSGPSYTGKEHSPVV
                     SLPIALEEVLDGVDAVVVSHVHSDHFDTTAREILPKDLPLLCQPEDEQTMRDLGFTDV
                     HPVAGTLWLDEISVSRTSGRHGSGAVLKDMGVVSGFFSTAPEPWRP"
BASE COUNT          240 a          344 c          376 g          213 t
ORIGIN      
        1 cgttgtcgag tacacggcgt cgtggcccga gaacgcgcgg tactacgcgc tcttccgcaa
       61 ggccgacggc agcgggtacg agatccccga acgcggcata cagtttgaaa tcaaggcggg
      121 gcagagcctt cccgacggcg cgacgccgcc cgacttctac gaggagggcg aggggctgat
      181 gacctggagg aacgttgtga cggacggcgg atcgttcgac cttgaaccgg cgcccggaat
      241 cgtgaccttc cgcgtgtgct cggtccgcgc ggccgaggcg ccggcggcct cctcgggagg
      301 atcaggctgc tcgacggacg ttgcgtcgcc tttagcgctg cttctggcga taccgctgct
      361 tcttctgcgg aaaatgcagc ggaaaacaca ccgcacatca caaggagggg atcagcgatg
      421 acgcaggaac ggaagaaggc ggaagccaaa ctggaggata acctgaagcc ggaggaactc
      481 aagaagaaaa agaagctcgg cgagcaagac gacgctgaac tggacggaat ctccggcggc
      541 gaggggatgg atacggggaa gatcataccg tagcgttttt cgaaagctgg aaagagattc
      601 gcgatctctt tccagctttt ttagggtgcg tttgctccag tcgtccaaag aacctgctcg
      661 ttgaaaattt ctcttgaccg tccgtctccc tgcggcgtat catcgggcac agaaggaggc
      721 ggtatatttt ggagctgcaa ctcatccgca atgccaccgt aaaaatgtcg ttcgcaggaa
      781 aaatgctgtt gctggacccg tacctggggg agaaggggtc ggggccttcc tacaccggga
      841 aggagcacag cccggtcgtt tcgctgccca tcgctcttga ggaggtgctc gacggggtgg
      901 atgccgtcgt cgtctcccat gtgcactccg accacttcga cacaacggcc cgggagattc
      961 ttccgaaaga tcttccgctg ctctgccagc ccgaggacga acagaccatg cgcgacctcg
     1021 gcttcaccga tgtacacccc gtcgccggaa cgctttggct ggacgaaatt tccgtctccc
     1081 gcacatcggg gcgccacggt tcgggcgcgg tgctgaagga catgggcgtc gtctcgggct
     1141 tcttcagcac cgcgcccgaa ccgtggcgcc ccg
//
LOCUS       DAOTIL010000973         1170 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminobacteriaceae bacterium isolate AMR_MDS_3026
            NODE_145663_length_1170_cov_1.884305, whole genome shotgun
            sequence.
ACCESSION   DAOTIL010000973 DAOTIL010000000
VERSION     DAOTIL010000973.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868050
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminobacteriaceae bacterium (wastewater metagenome)
  ORGANISM  Aminobacteriaceae bacterium
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae.
REFERENCE   1  (bases 1 to 1170)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1170)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:19:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,224
            CDSs (total)                      :: 3,198
            Genes (coding)                    :: 3,184
            CDSs (with protein)               :: 3,184
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1170
                     /organism="Aminobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_145663_length_1170_cov_1.884305"
                     /isolate="AMR_MDS_3026"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:3034609"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            100..1128
                     /gene="tsaD"
                     /locus_tag="P5201_14955"
     CDS             100..1128
                     /gene="tsaD"
                     /locus_tag="P5201_14955"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008711180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     transferase subunit TsaD"
                     /protein_id="HRV99897.1"
                     /translation="MSLPEESFLTLGMESSCDDTAVAVLRGGRQVEAELLSSQLEDHV
                     PFGGVIPEFAARRHLEAFLPLTEEALRRAGVADPRREIGLVAVTAGPGLMGSLLVGVM
                     AAKALSLAWDVPLLGVNHLEGHIFANVVSHPELSPPFLCMVVSGGHTEIVLVKKFGTY
                     VFLGGTRDDAAGEAYDKVAKVLGLPYPGGPVVDKLAASGNPDAFDFPSPLKGSPEIVF
                     SFSGLKTAVLTRIKKLRNEGIEIPVEDVCASFQRAVTDSLMTKLRLASRRTGVRSVSI
                     SGGVAANSALRNALLQEKGWKVFLPPKNRCTDNAVMIAAAGYSAFRRGARSSLDLAPD
                     PSWELWKK"
BASE COUNT          204 a          367 c          372 g          227 t
ORIGIN      
        1 ggcggccttc gctgtcgccg tgcccctcgc cgcggtgaaa gggacaacgc ggactgtctc
       61 cgtcggcgac tacggcacgg agctggagtt cccgatacga tgagcctccc ggaagagtct
      121 tttcttactc tcggaatgga gagcagctgc gacgacacgg cggtcgccgt ccttcgcggg
      181 ggaaggcagg tagaggcgga gctgctctcc agccagcttg aggaccacgt tccgttcgga
      241 ggggtcatcc ccgaattcgc ggcgcggcgg cacctggaag cctttcttcc cctcacggag
      301 gaggcgctcc gcagggcggg cgtcgccgac ccccggcggg agatagggct cgtcgccgtc
      361 actgcggggc cgggactcat ggggtcgctg ctcgtcggcg tcatggcggc gaaagcgctc
      421 tcccttgctt gggacgtccc gctcctcggc gtgaatcacc ttgaagggca tatcttcgcc
      481 aatgtcgtct cgcacccgga gctctcccct ccctttctct gcatggttgt ctcggggggg
      541 cacacggaga tcgtccttgt caagaagttc gggacctacg tctttctcgg cgggacccgc
      601 gacgacgcgg ccggagaagc gtacgacaag gtcgcgaagg tcctcggcct cccgtacccc
      661 ggaggacccg tggtggacaa gcttgccgcc tcgggaaatc ccgacgcttt cgactttccc
      721 tcgccgctca agggaagccc ggagatcgtc ttcagcttca gcggactcaa aactgcggtg
      781 ctcacccgga taaagaagct acggaacgaa gggatcgaga ttcccgtcga ggacgtctgc
      841 gcctctttcc agagggccgt gaccgactca ttgatgacga agctgcgtct cgcctcccgg
      901 cggacgggag ttcgttccgt ctccatctcg ggcggcgttg cggcgaacag cgcgctgagg
      961 aatgcgcttc ttcaggaaaa agggtggaag gtcttccttc cgccgaaaaa ccgctgtacc
     1021 gacaacgccg tcatgatagc cgctgcaggc tattccgcct tccggagagg tgcgcgctct
     1081 tcacttgatc tggcgccgga cccgtcgtgg gagctttgga aaaagtaatt cttccagaat
     1141 gtgtcaagat acgttgaaaa aagcatctct
//
LOCUS       DAOTIL010000974         1166 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminobacteriaceae bacterium isolate AMR_MDS_3026
            NODE_146304_length_1166_cov_3.920792, whole genome shotgun
            sequence.
ACCESSION   DAOTIL010000974 DAOTIL010000000
VERSION     DAOTIL010000974.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868050
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminobacteriaceae bacterium (wastewater metagenome)
  ORGANISM  Aminobacteriaceae bacterium
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae.
REFERENCE   1  (bases 1 to 1166)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1166)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:19:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,224
            CDSs (total)                      :: 3,198
            Genes (coding)                    :: 3,184
            CDSs (with protein)               :: 3,184
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1166
                     /organism="Aminobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_146304_length_1166_cov_3.920792"
                     /isolate="AMR_MDS_3026"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:3034609"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..874
                     /gene="miaA"
                     /locus_tag="P5201_14960"
     CDS             <1..874
                     /gene="miaA"
                     /locus_tag="P5201_14960"
                     /EC_number="2.5.1.75"
                     /inference="COORDINATES: protein motif:HMM:TIGR00174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0052381 - tRNA dimethylallyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="tRNA (adenosine(37)-N6)-dimethylallyltransferase
                     MiaA"
                     /protein_id="HRV99898.1"
                     /translation="VEQAREIVALGADKISPDIRRRTIHHMIDVADPDQVFTAADFVS
                     GCVDAVERIRARGRIPLFAGGTPFYFEALFGGMLSEELPKDEDLRRELEQIAAEQGAG
                     ALHATLRSVDPESAGRLHENDVRRVVRALEICRLTGKTVAEAWSERKKMTPPKEYDVL
                     YVGLTRERRFLFESIERRVGEQFASGFVEEVEWLLNNGYDERFPSMQGFGYKDILEYL
                     RGRCSREEAAERDIRQTKAFSRRQMTWFGKFSPILWYDTVDCSMTKLVDTIENDVRHY
                     PGRWSFVNGAQEGN"
     gene            852..>1166
                     /locus_tag="P5201_14965"
     CDS             852..>1166
                     /locus_tag="P5201_14965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006583414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRV99899.1"
                     /translation="MEHKRVIETASPTGFCFGVQRAIESLEKRIEECASSASGSDGRV
                     YSIGMPIHNPQEVERLSRKGLRVVDRIEDIPCGSAVFVRAHGVPPSVKEELISRCGAE
                     NVT"
BASE COUNT          251 a          295 c          376 g          244 t
ORIGIN      
        1 tgtggagcag gcccgggaga tcgtcgcgct cggcgcggac aagatttcgc cggatattcg
       61 acggcgaact atccatcaca tgatcgacgt ggcggacccg gaccaggtgt tcaccgcggc
      121 ggacttcgtc tcaggctgcg tggacgcggt ggaaagaata cgcgcgcgcg ggcggattcc
      181 tctctttgcg ggaggcacgc ccttctactt cgaagcgctc ttcggcggaa tgctctccga
      241 ggaactgccc aaggacgaag atctgcggag agagctggag caaatcgccg cggaacaggg
      301 agcgggcgcg ctgcatgcga cgcttcgttc ggtcgaccct gagagcgccg gaaggctcca
      361 tgagaacgat gtccggcggg tcgtccgcgc gttggagatc tgtcgtttga ccggaaagac
      421 cgtcgcggag gcgtggagcg aaagaaaaaa gatgacgccg ccgaaagagt atgacgttct
      481 gtacgtcggc ctgacgcgcg aacgccgctt tctcttcgaa agcatcgaac ggcgggtcgg
      541 agagcagttc gcctctgggt ttgtagaaga ggtggagtgg cttctcaaca acggatacga
      601 cgagcgtttt ccgtccatgc aggggttcgg atataaggat attctggagt atctgcgggg
      661 gcgctgctcg cgcgaagagg cagccgaacg ggatatacgg caaacaaaag cattttctcg
      721 aaggcagatg acctggtttg ggaaattttc tccaattcta tggtatgata ctgtagattg
      781 ttcgatgacg aaactcgtcg atacgatcga aaacgatgta cggcactatc ctgggagatg
      841 gtctttcgtc aatggagcac aagagggtaa ttgagactgc gtcccccacc ggtttttgct
      901 tcggtgttca acgggccata gaaagtctgg agaaacgcat tgaagagtgc gcttcctcgg
      961 cttccggaag cgatggaaga gtctattcca tcggaatgcc gatccacaat cctcaggaag
     1021 tggagcgtct ttcccggaaa gggcttcgag ttgtcgaccg gatagaggat attccatgcg
     1081 gctccgctgt tttcgtccgt gcgcacggcg ttcctccctc tgtgaaggag gagctcattt
     1141 cgcggtgcgg cgccgagaat gtcacc
//
LOCUS       DAOTIL010000975         1165 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminobacteriaceae bacterium isolate AMR_MDS_3026
            NODE_146547_length_1165_cov_2.641441, whole genome shotgun
            sequence.
ACCESSION   DAOTIL010000975 DAOTIL010000000
VERSION     DAOTIL010000975.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868050
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminobacteriaceae bacterium (wastewater metagenome)
  ORGANISM  Aminobacteriaceae bacterium
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae.
REFERENCE   1  (bases 1 to 1165)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1165)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:19:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,224
            CDSs (total)                      :: 3,198
            Genes (coding)                    :: 3,184
            CDSs (with protein)               :: 3,184
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1165
                     /organism="Aminobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_146547_length_1165_cov_2.641441"
                     /isolate="AMR_MDS_3026"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:3034609"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1138)
                     /locus_tag="P5201_14970"
     CDS             complement(<1..1138)
                     /locus_tag="P5201_14970"
                     /inference="COORDINATES: protein motif:HMM:NF022655.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2993 domain-containing protein"
                     /protein_id="HRV99900.1"
                     /translation="MKNTLFAYILLLSLCAVAPAGGEHVPVFPPDSGAQDNGGRLLST
                     MVSLLLPESMELRMDEPPDDAGNVRNISVALRGALLTGFRVERIALESSFLELSPPSS
                     WKPGDRSSLAVRGALRTNTEIVLLEEDINAALEPYATEQYRLAVDLKPGGIRLRVNFT
                     AGHLGGGSLATVDAGLEIRRGSQLWLKNPRILVNGDDQTDAVVNELLKIQPFVDFAAF
                     GFPPNLVAASVDENALRLSSRTSPKPVEGLLYRYEALPGVSPPVVPNAVLKYSPELFR
                     NGDIVFVNGKRWRTKIVRLFDKSPRGFAHAGIVRIVDGLPHVVHASPELGKVGMQSME
                     DFFSFENVDYARVYRFSGDPAVAEAASLRALEYCTREIPFDDRFD"
BASE COUNT          229 a          361 c          370 g          205 t
ORIGIN      
        1 tatcgaaccg gtcgtcgaag gggatttcgc gcgtacagta ttcgagcgcc cgcaggctcg
       61 ccgcctccgc gacggcggga tcgccggaga agcggtatac ccgcgcgtag tccacattct
      121 cgaaggagaa gaagtcctcc atcgactgca tccccacctt gcccagctcg ggcgacgcgt
      181 ggacgacatg cggaagaccg tcgacgatgc gcacgattcc ggcgtgagcg aatccgcggg
      241 ggcttttgtc gaagaggcgg acaatcttcg ttctccacct cttgccgttg acgaagacga
      301 tgtccccgtt gcggaagagt tccggcgaat acttcagaac cgcattcggc acgacgggag
      361 gagagactcc cgggagcgcc tcgtaacggt agagcagccc ttcgacaggc ttcggagaag
      421 tccgggagga gagtcggagc gcgttctcgt ccacggaggc ggcgacaaga ttcggcggaa
      481 agccgaaagc ggcgaagtcg acgaagggct gaatcttcag cagctcgttc actaccgcgt
      541 ccgtctggtc gtcgccgttg acgaggatac gcggattctt cagccagagc tggctccccc
      601 ttctgatttc aagtcctgcg tccaccgttg ccagactccc gccccccaga tgccccgccg
      661 tgaagttgac acgaaggcgg atacctccgg gcttgagatc gactgcgagt cggtactgct
      721 ccgtcgcgta cggttcgagc gcagcgttga tatcctcttc aaggaggact atttccgtgt
      781 tcgtccgcag cgcgcctcgc acggcaagcg agctcctgtc gcctggcttc cacgacgacg
      841 ggggagagag ctccaggaag gaggactcaa gcgcgatccg ctccacgcgg aagccggtca
      901 gcaaggctcc gcgcagcgca acggaaatgt tgcggacatt ccccgcgtcg tccgggggtt
      961 cgtccatgcg aagctccatc gactcgggaa gcagcagcga gaccatcgtc gaaagaagac
     1021 gcccgccgtt gtcctgcgct ccggaatccg gcgggaagac cggtacgtgc tcccctcccg
     1081 ccggggcgac ggcgcagagg gaaagaagga gtatgtacgc gaaaagggtg tttttcacac
     1141 ggcacctcct tgacatgttc gtttc
//
LOCUS       DAOTIL010000976         1164 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminobacteriaceae bacterium isolate AMR_MDS_3026
            NODE_146710_length_1164_cov_3.044184, whole genome shotgun
            sequence.
ACCESSION   DAOTIL010000976 DAOTIL010000000
VERSION     DAOTIL010000976.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868050
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminobacteriaceae bacterium (wastewater metagenome)
  ORGANISM  Aminobacteriaceae bacterium
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae.
REFERENCE   1  (bases 1 to 1164)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1164)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:19:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,224
            CDSs (total)                      :: 3,198
            Genes (coding)                    :: 3,184
            CDSs (with protein)               :: 3,184
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1164
                     /organism="Aminobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_146710_length_1164_cov_3.044184"
                     /isolate="AMR_MDS_3026"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:3034609"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..304)
                     /locus_tag="P5201_14975"
     CDS             complement(<1..304)
                     /locus_tag="P5201_14975"
                     /inference="COORDINATES: protein motif:HMM:NF014573.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HRV99901.1"
                     /translation="MRLNIFNCSPRGKRSNTKVLTNAFAEGFLRGAAGAPELNEVREF
                     FISEPDDFRRAAEAFGEPSAALIAFPVYVDAMPAIAKSFIEEIAAFKGRCSSTRMLF"
     gene            complement(285..833)
                     /locus_tag="P5201_14980"
     CDS             complement(285..833)
                     /locus_tag="P5201_14980"
                     /inference="COORDINATES: protein motif:HMM:NF015325.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HRV99902.1"
                     /translation="MRILFVDGNPSAEDAVFGERLAAMEHGVAGHDVTRFCLRGKAIH
                     SCIGCFACWMRTPGTCIFQDDHVELLRLFLKSDVAVFASPLIAGFYSALLKTAIDRVI
                     PLVLPYIEFADGECRHPLRYGRAMPKMGFLYEPEEDTDEEDLRIVSTAFERFARNGHT
                     RFLFSRPLSEDPEEVRHAIEHL"
BASE COUNT          294 a          356 c          286 g          228 t
ORIGIN      
        1 gaaagagcat tctcgtcgac gagcagcgcc ccttgaaggc ggcgatctct tcaatgaagg
       61 atttcgcaat agcgggcatc gcgtccacat agaccggaaa ggcgatcagg gcagccgacg
      121 gctctccgaa agcttccgcc gcccgccgga agtcatccgg ttccgatata aaaaactccc
      181 tgacctcgtt cagttccggc gcacccgccg ctccccggag aaagccctcc gcaaaggcgt
      241 ttgtcaacac cttggtgttg ctcctcttgc cccgcggcga gcagttaaag atgttcaatc
      301 gcatggcgca cctcctcggg atcctcactc aaaggccggg agaacaggaa acgcgtgtgc
      361 ccgttgcggg cgaaacgctc gaacgcggtg gagacaatcc ggagatcctc ctcgtccgta
      421 tcctcttccg gttcatagag aaagcccatc ttcggcatgg cacggccgta ccgcagcggg
      481 tgacggcact ccccgtccgc aaactcgatg tacggcagaa caagcggaat gacgcggtcg
      541 atcgccgtct tcagcaacgc ggagtaaaaa cccgcgatca gaggagaagc aaagacggca
      601 acatccgatt tcagaaacaa acgcagcagc tcgacgtgat catcctggaa gatacatgtc
      661 cccggggtgc gcatccaaca cgcgaaacaa ccgatgcagg agtgaatcgc ctttccccgg
      721 agacagaacc tcgtgacatc gtgccccgca acaccgtgct ccatcgccgc aagacgctcg
      781 ccgaaaacgg cgtcttccgc gcttggattc ccgtcgacaa acaagattct caatacgcaa
      841 cacctcgcca tccttacaaa tccccggaac gattggctga aaaatattcc agagcgcccc
      901 gggctgaacg tccacttcca tctttgattg cattcttttc tcaggtgcaa ataaagcgtc
      961 attataatac aggcgcttcg cctcgaatac agttctctga tacaatacta cagaattctt
     1021 ttattttggg cgatacacaa cacaccatgg atgacgctac tgagaaaaat cacggatacg
     1081 cgaaaccgtc cgctttttca cagcatcgaa aaagggggga tcagcgtgag gaaaacattc
     1141 cttctcttcg tatgtctttt gctc
//
LOCUS       DAOTIL010000977         1163 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminobacteriaceae bacterium isolate AMR_MDS_3026
            NODE_146888_length_1163_cov_3.370939, whole genome shotgun
            sequence.
ACCESSION   DAOTIL010000977 DAOTIL010000000
VERSION     DAOTIL010000977.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868050
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminobacteriaceae bacterium (wastewater metagenome)
  ORGANISM  Aminobacteriaceae bacterium
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae.
REFERENCE   1  (bases 1 to 1163)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1163)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:19:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,224
            CDSs (total)                      :: 3,198
            Genes (coding)                    :: 3,184
            CDSs (with protein)               :: 3,184
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1163
                     /organism="Aminobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_146888_length_1163_cov_3.370939"
                     /isolate="AMR_MDS_3026"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:3034609"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..630
                     /locus_tag="P5201_14985"
     CDS             <1..630
                     /locus_tag="P5201_14985"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HRV99903.1"
                     /translation="GAQRTEAMEALFGLRSLSGGKILLGGKEADLSTPIKAKRRGLAF
                     LTEERRATGIFPVLSVEFNLLVAHLGNYVSRMRLLEQKRMRRDARESIERLAVKTPSS
                     RSLIRDLSGGNQQKVLFARWLLINPEILILDEPTRGIDVGAKYEIYSIMSELASQGKS
                     IIMISSEMPELLGMTDRILVMSQGRLAGIVETKETSEEDIMRLATKYVA"
     gene            662..>1163
                     /gene="mglC"
                     /locus_tag="P5201_14990"
     CDS             662..>1163
                     /gene="mglC"
                     /locus_tag="P5201_14990"
                     /inference="COORDINATES: protein motif:HMM:NF014684.2"
                     /note="ABC transporter; functions in galactose transport;
                     part of MglA2C2B transporter complex; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-methylgalactoside transporter permease"
                     /protein_id="HRV99904.1"
                     /translation="MESKKTVLGSSVHFLSQNAIFVVLVVLVLLIAVVDPRFLSFAIL
                     RDVLMQSSTRAIMALGVAFILITGGVDLSSGRMVGFAAVIAASMLQIPEYARRFYPDL
                     PHLPVFLPIILAVAACMLFGMLNGLIVAKLQVPPFITTLGTMVVVYGINSIYFDMPPN
                     NSQPIGG"
BASE COUNT          219 a          325 c          339 g          280 t
ORIGIN      
        1 ggcgcgcagc gcaccgaagc catggaggcg ctcttcggtc tgcgctctct ctcgggggga
       61 aagattctcc ttggcggcaa ggaagcggac ctttcaacgc cgataaaggc gaagcggcgc
      121 gggctggcct tccttaccga agagcgccgg gccaccggta tctttccggt cctctcggtg
      181 gagttcaacc ttcttgtggc gcacctcgga aactatgtaa gccgcatgcg gctgctcgaa
      241 cagaaacgga tgcgcaggga tgcccgtgag agtatcgaac ggcttgccgt caagacgcct
      301 tcgtcccgga gcctgatacg cgatctttcc ggcggcaatc agcaaaaggt gctctttgcc
      361 cgctggcttc tgatcaaccc cgagattctc atcctcgacg aaccgacgcg gggaatcgac
      421 gtcggcgcga agtacgagat ttactcgatc atgtcggagc ttgcatccca ggggaagagt
      481 atcatcatga tctcctccga gatgccggag cttctcggga tgacggacag aatcctcgtg
      541 atgagccagg ggaggcttgc gggaatcgtg gaaacgaagg agacttccga agaggatatc
      601 atgcggcttg ccacgaagta cgtggcgtag cttgagctga cgagggagga aggacattcc
      661 tatggagtcg aaaaagactg ttcttggatc ttcagtgcat tttctttcac agaacgccat
      721 cttcgtcgtt ctggtcgtgc tggtgttact tatcgccgtg gtggacccgc gttttctctc
      781 attcgctatt ttgcgggacg tccttatgca gagctccacg cgggcgatca tggcccttgg
      841 cgtggcgttt attcttatta ccggaggcgt tgatctctcc tccgggcgta tggtgggctt
      901 cgcggcggtc atcgccgcct cgatgcttca gatacccgag tatgcgcggc gcttctaccc
      961 cgatcttccg catttgcctg tttttctccc gattattctt gcagtggccg cctgcatgct
     1021 cttcggaatg ctcaacggac tgattgtggc gaagctccaa gtgcctccgt tcattacgac
     1081 gctcggtacg atggtcgtcg tctacggaat caattcgatc tatttcgaca tgcctccgaa
     1141 taattcgcag cctataggcg gcc
//
LOCUS       DAOTIL010000978         1162 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminobacteriaceae bacterium isolate AMR_MDS_3026
            NODE_147100_length_1162_cov_3.184282, whole genome shotgun
            sequence.
ACCESSION   DAOTIL010000978 DAOTIL010000000
VERSION     DAOTIL010000978.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868050
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminobacteriaceae bacterium (wastewater metagenome)
  ORGANISM  Aminobacteriaceae bacterium
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae.
REFERENCE   1  (bases 1 to 1162)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1162)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:19:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,224
            CDSs (total)                      :: 3,198
            Genes (coding)                    :: 3,184
            CDSs (with protein)               :: 3,184
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1162
                     /organism="Aminobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_147100_length_1162_cov_3.184282"
                     /isolate="AMR_MDS_3026"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:3034609"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1132)
                     /locus_tag="P5201_14995"
     CDS             complement(<1..1132)
                     /locus_tag="P5201_14995"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="S9 family peptidase"
                     /protein_id="HRV99905.1"
                     /translation="MNIFVQKIGAEEAVRVTSATERDIPAYFWSGNDRIVYLQDRGGD
                     ENYRLYAVSSDGTESRELTPFENTRVTIVDSLEEQDDFLLIGMNRRDQRLFDVYRLNL
                     ATGDLTLLEENPGDIAGWMTDHDGKLRVAVRSDGVNTSLLYRDKEDEPFRNILTTDFR
                     ESVSPLFFTFDNKKLYVASNLGRDKSAIFVFNPQTVEHEELLFEHPDVDVSALFRSRK
                     RKVITGTGFTTDRFRYHFFDEQRAALQKELEERLPDDEVVVASMSRDETKVLVYAGSD
                     RTRGTYFFFDRETKDFRKLAELAPWLPKERMAPMKHVSYTSRDGLTIHGYLTLPLGKE
                     PKNLPVVIHPHGGPWVRDGWGFDPQVQFLANRGLAVLQMNFRG"
BASE COUNT          195 a          376 c          350 g          241 t
ORIGIN      
        1 agccgcggaa gttcatctgc agcactgcga gccccctgtt ggcgaggaac tgtacctgcg
       61 ggtcgaaccc ccatccgtcg cgtacccacg ggcctccgtg cgggtggatc accacgggga
      121 ggttcttcgg ctcctttccg agagggagcg tcaggtagcc gtggatcgtc agcccgtccc
      181 tcgatgtgta ggacacatgc ttcatcggcg ccatgcgctc tttcggcagc catggcgcca
      241 gctccgccag cttccggaag tccttcgtct ctctgtcgaa aaagaagtag gtcccgcggg
      301 tccggtcgct ccccgcgtac accagcactt tcgtctcgtc ccggctcatg gaggcgacga
      361 cgacctcgtc gtcaggaagg cgctcttcca gctccttctg gagcgcggct cgctgctcgt
      421 cgaagaagtg gtagcgaaag cggtccgttg tgaagcccgt tccggtgatc acctttcgct
      481 tccgggagcg gaaaagcgca ctcacgtcaa cgtccgggtg ttcgaagagc aactcctcgt
      541 gctcgacggt ctgcgggttg aagacgaaga tcgccgactt gtcccgcccg aggttcgacg
      601 cgacgtagag ttttttgttg tcgaacgtga agaagagcgg cgacacgctt tctcggaagt
      661 ccgtggtcag gatgttccgg aagggctcgt cctccttgtc gcggtagaga agcgacgtgt
      721 tcactccgtc ggagcgcacc gcgacccgca actttccatc gtggtccgtc atccaccccg
      781 cgatatcccc cgggttctcc tccagcagcg tcagatctcc cgtcgccagg ttcagccgat
      841 agacgtcgaa gaggcgctgg tcccttcggt tcatgccgat caggagaaag tcgtcctgct
      901 cctcaaggct gtcgacgatg gtcacgcggg tattctcgaa cggcgtcagc tcgcgcgact
      961 cggtcccgtc ggacgagacg gcgtagagcc ggtagttctc atcgccgccc ctgtcctgaa
     1021 ggtagacgat cctgtcgttc cccgaccaga agtaggccgg gatatcccgc tccgtcgcgg
     1081 aggttacgcg cacggcttcc tccgcgccga ttttctggac gaagatgttc atccgccgct
     1141 cccacggctt catgaacgag at
//
LOCUS       DAOTIL010000979         1160 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminobacteriaceae bacterium isolate AMR_MDS_3026
            NODE_147480_length_1160_cov_3.467873, whole genome shotgun
            sequence.
ACCESSION   DAOTIL010000979 DAOTIL010000000
VERSION     DAOTIL010000979.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868050
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminobacteriaceae bacterium (wastewater metagenome)
  ORGANISM  Aminobacteriaceae bacterium
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae.
REFERENCE   1  (bases 1 to 1160)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1160)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:19:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,224
            CDSs (total)                      :: 3,198
            Genes (coding)                    :: 3,184
            CDSs (with protein)               :: 3,184
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1160
                     /organism="Aminobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_147480_length_1160_cov_3.467873"
                     /isolate="AMR_MDS_3026"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:3034609"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>1160
                     /locus_tag="P5201_15000"
     CDS             <1..>1160
                     /locus_tag="P5201_15000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008521131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="urocanate hydratase"
                     /protein_id="HRV99906.1"
                     /translation="ETELALKNALRYVPEMYHKELIPEFLEELRTRGRIYAYRFRPEG
                     RIKGLPVHQYKGKCLAGKAFQVMIDNNLDFEIALYPYELVTYGETGQVCQNWLQYRLI
                     KRYLEELTEETTLVVESGHPLGLFKSRPDAPRVILTNGMLVGLFDNPAEWHRGMQLGV
                     TNYGQMTAGGWMYIGPQGIVHGTFNSILNAARQRLGVGEKENLAGILFVSSGLGGMSG
                     AQPKAVEIAGGVGIIAEVDASRIETRHSQGWVAKVTGSCKEAFDLAAEACATKQSLSI
                     AYHGNIVDLLQYAAEHDISIPLLSDQTSCHAPYDGGYCPQGLTFDERTKMLSKNPEEF
                     RRLVDISLRKHYEFIKTLVDRGTYFFDYGNSFMRAVFDAGVPEICKNGENTY"
BASE COUNT          253 a          364 c          346 g          197 t
ORIGIN      
        1 cgagacggag cttgcattga agaacgccct ccgctacgtg cccgaaatgt accacaagga
       61 gctgatccct gaattcctgg aggagcttcg taccaggggg cgcatctacg cctacaggtt
      121 ccggcccgag gggcggataa agggcctccc ggtgcatcag tacaagggga agtgcctcgc
      181 cgggaaggcg ttccaggtca tgatcgacaa caacctcgac ttcgaaatcg ccctctaccc
      241 gtacgaactc gtcacgtacg gcgagaccgg acaggtctgc cagaactggc ttcagtatcg
      301 tttgatcaaa aggtaccttg aagagctgac cgaggagacg acgctcgtcg tcgagagcgg
      361 ccaccccctc ggccttttca aatcgcgtcc ggacgcgccc cgggtcattc tgactaacgg
      421 catgctcgtc ggccttttcg acaacccggc ggagtggcac agagggatgc agctcggcgt
      481 gacgaactac ggacagatga cggccggagg gtggatgtac atcggccccc agggcatcgt
      541 ccacggtacg ttcaacagca ttctcaacgc cgcccggcag cggctcggcg tcggtgagaa
      601 agaaaatctc gcgggaatcc tcttcgtctc ctccggactc ggcggcatga gcggcgcgca
      661 gccgaaggcc gtggaaatcg caggcggcgt cggcatcatc gccgaggtgg acgcttcgcg
      721 catcgagacg cgccacagcc aagggtgggt agccaaggtc accggaagct gcaaggaagc
      781 cttcgatctc gccgccgagg catgcgcgac aaaacagtcg ctttcgatcg cgtaccacgg
      841 caacatcgtg gatctgctgc agtacgcggc agaacacgac atttccattc cgctgctctc
      901 cgaccagacc tcctgccacg ctccctacga cgggggctac tgccctcagg gactgacctt
      961 cgacgagcgc accaagatgc tctcgaagaa cccggaggag ttccgccgcc ttgtggatat
     1021 atccctgagg aagcactacg agttcatcaa gacgctcgtc gacaggggaa catacttctt
     1081 cgactacggc aactcgttca tgcgcgcggt cttcgacgcg ggagtccccg agatctgcaa
     1141 aaacggcgag aacacgtacg
//
LOCUS       DAOTIL010000980         1160 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminobacteriaceae bacterium isolate AMR_MDS_3026
            NODE_147489_length_1160_cov_3.259729, whole genome shotgun
            sequence.
ACCESSION   DAOTIL010000980 DAOTIL010000000
VERSION     DAOTIL010000980.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868050
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminobacteriaceae bacterium (wastewater metagenome)
  ORGANISM  Aminobacteriaceae bacterium
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae.
REFERENCE   1  (bases 1 to 1160)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1160)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:19:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,224
            CDSs (total)                      :: 3,198
            Genes (coding)                    :: 3,184
            CDSs (with protein)               :: 3,184
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1160
                     /organism="Aminobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_147489_length_1160_cov_3.259729"
                     /isolate="AMR_MDS_3026"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:3034609"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..818
                     /locus_tag="P5201_15005"
     CDS             <1..818
                     /locus_tag="P5201_15005"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HRV99907.1"
                     /translation="RILLAEDNITNQRVALGMLKKIGVTADAAENGEAALEALASCSY
                     DLVLMDCRMPVMDGYEATRRIRRMEGDVRNIPVIALTAYAMPGDREKCLEAGMNDYLA
                     KPIIRDALLAVLERWLPRREKEEGPFEDVATEGAREMEAQHLESVWDRDVLLERLLGD
                     EEMLEEIITGFLEDAPAQLSALSEALATGDAASAEMSAHAIKGAAAGIGAERVRCAAF
                     AVENAVRNGAMDKAASLIGALELEFGRLKRFIEERTRPNEVGDVSPENPVAKE"
     gene            981..>1160
                     /locus_tag="P5201_15010"
     CDS             981..>1160
                     /locus_tag="P5201_15010"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRV99908.1"
                     /translation="MAMLALAAMLTLSDVELYYEVHGDGPPLLLIAGMASDSQSWGSS
                     APSLAEHFTVICPDNR"
BASE COUNT          234 a          306 c          387 g          233 t
ORIGIN      
        1 gacgaatctt gctggccgag gataatatca ctaaccagag ggttgccctg ggcatgctga
       61 agaagatcgg cgtgacggcg gatgccgccg aaaacggcga agctgcgctc gaagcccttg
      121 cctcatgctc ttacgacctt gtgttgatgg attgccggat gcccgtgatg gatggatacg
      181 aggcaacccg cagaatccgc cgcatggagg gcgacgtccg gaatattccc gttattgcac
      241 tgactgcgta cgccatgccc ggagacaggg agaaatgcct tgaagcgggc atgaacgact
      301 acctcgccaa gccgattatt cgcgatgcgc tgctcgctgt gctggaacgc tggctccccc
      361 gccgggagaa ggaagaaggg ccgtttgagg acgttgcgac ggaaggagcc cgggaaatgg
      421 aagcgcaaca tctggagtcg gtctgggaca gggacgtcct gttggaacgt cttctcgggg
      481 atgaagagat gctcgaagag atcatcacag gctttctgga ggacgcgccg gcgcagcttt
      541 ctgcgctttc cgaagctctt gcaacgggag acgccgcttc cgcggagatg tccgcgcacg
      601 caatcaaggg agctgcggcg ggtatcggag cggagcgggt ccgatgcgcg gcgttcgcgg
      661 tagagaatgc cgtacgcaac ggagcgatgg acaaagccgc ttctcttata ggagcgctcg
      721 agctcgagtt cgggaggttg aagcgcttca tcgaagaaag aacccgtccg aacgaagtgg
      781 gagacgtttc cccggaaaac ccggttgcga aagagtgacg cgtggaacgc tttctttcgg
      841 gaggcgtttg gtctctttct gaaaagagcc gccgcgtggt atagtaatgc cgtccgcccg
      901 tgcccttatg gaatgaccgg agaacggcgg gttatggaag tctttttccc gctcttcatt
      961 tatctgaaag gggttttgtg atggcgatgt tggcgctggc cgcaatgctc acgttgagcg
     1021 atgtcgagct gtactacgag gtgcacggcg acgggccgcc tctgctgctg atcgccggga
     1081 tggcgtcgga ctcgcaaagc tggggctctt cggcgccctc gcttgcggag cattttaccg
     1141 tcatctgccc cgacaaccgg
//
LOCUS       DAOTIL010000981         1160 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminobacteriaceae bacterium isolate AMR_MDS_3026
            NODE_147534_length_1160_cov_2.463348, whole genome shotgun
            sequence.
ACCESSION   DAOTIL010000981 DAOTIL010000000
VERSION     DAOTIL010000981.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868050
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminobacteriaceae bacterium (wastewater metagenome)
  ORGANISM  Aminobacteriaceae bacterium
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae.
REFERENCE   1  (bases 1 to 1160)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1160)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:19:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,224
            CDSs (total)                      :: 3,198
            Genes (coding)                    :: 3,184
            CDSs (with protein)               :: 3,184
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1160
                     /organism="Aminobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_147534_length_1160_cov_2.463348"
                     /isolate="AMR_MDS_3026"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:3034609"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..951)
                     /locus_tag="P5201_15015"
     CDS             complement(<1..951)
                     /locus_tag="P5201_15015"
                     /inference="COORDINATES: protein motif:HMM:NF015445.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter substrate-binding protein"
                     /protein_id="HRV99909.1"
                     /translation="MKKTLAVLLTIAILCTGVSAAFAAKVNWRLVTHASPGTEQQRIA
                     EVFCETVKTLSGGEFVIEPYAAGVLFPVFESFDFLANGVIDASMVYSAYWTGKDTGFT
                     LTTLPGCPLSTYAEGAYLVEQLFPYFEKLYAKYGITYLGHAMVSPINEQLISVVPINT
                     LEDIKGKKIRTSGFGARYYSALGATTVSLSGPEIYTALQTKNLDAAEWTFWDENMRMG
                     LHEVANYVLDPALQNGTCEYFPLVVNPDKWNALPQNYKDIVMVARDRIRYLSAMVYVH
                     EIKSREKWKTMPNLQIVSWSLEDQKKARAIGQQLVLEECNK"
BASE COUNT          294 a          318 c          264 g          284 t
ORIGIN      
        1 cttgttgcac tcctcaagaa cgagttgctg accgatagcc cgagcttttt tctgatcctc
       61 aagactccag ctcactatct gaaggttcgg catggttttc cacttctccc gcgacttgat
      121 ctcatgtaca tagaccatcg ccgaaagata ccggatacga tcccgtgcaa ccatgacgat
      181 atccttgtag ttctggggta gcgcattcca cttgtcggga ttgaccacca gcgggaaata
      241 ctcgcacgta ccgttctgca acgcagggtc aagaacatag ttggcaacct cgtggaggcc
      301 catacgcata ttctcatccc agaatgtcca ctcggcggca tcaaggttct tcgtctgcag
      361 agccgtataa atttccgggc cggagagcga aacggtagtc gcaccaaggg cgctgtaata
      421 gcgggcgcca aaaccggacg tacggatctt cttccccttg atatcctcca gagtgtttat
      481 cggaacgacg gagataagct gttcattgat ggggctgacc atagcatgtc caaggtatgt
      541 tatgccatat ttcgcataca acttttcaaa ataggggaaa agttgttcga cgagatatgc
      601 gccttccgca taggtactca gaggacatcc gggcagagtc gtcagggtga aaccggtatc
      661 cttccccgtc cagtaggcgc tgtacaccat cgaagcatcg ataacgccgt tcgccaagaa
      721 atcaaaggat tcgaaaacgg ggaaaagaac cccggccgcg tacggttcga taacgaactc
      781 accgccggag agcgttttca ccgtttcgca aaaaacctcc gctattcgct gctgttccgt
      841 tccgggactg gcatgcgtca ctaatctcca gttcactttc gccgcaaaag ccgcgcttac
      901 acctgtgcat agtatagcta tggttaagag taccgcgaga gtctttttca tccttcaaat
      961 acctcctttt ctggaatcgt tttgcttttg attgttcagc ttcagttcca caacttatcc
     1021 tcactctctc tgaacaaacc tccctgaaat accacctcct ttcctcatct acataccaag
     1081 aacatacctc ggaagccaga actatggacg gatagaggaa tatcagcgtg atgcatacaa
     1141 tctggagcag caaaaaaggt
//
LOCUS       DAOTIL010000982         1160 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminobacteriaceae bacterium isolate AMR_MDS_3026
            NODE_147537_length_1160_cov_2.447059, whole genome shotgun
            sequence.
ACCESSION   DAOTIL010000982 DAOTIL010000000
VERSION     DAOTIL010000982.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868050
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminobacteriaceae bacterium (wastewater metagenome)
  ORGANISM  Aminobacteriaceae bacterium
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae.
REFERENCE   1  (bases 1 to 1160)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1160)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:19:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,224
            CDSs (total)                      :: 3,198
            Genes (coding)                    :: 3,184
            CDSs (with protein)               :: 3,184
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1160
                     /organism="Aminobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_147537_length_1160_cov_2.447059"
                     /isolate="AMR_MDS_3026"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:3034609"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..448
                     /locus_tag="P5201_15020"
     CDS             <1..448
                     /locus_tag="P5201_15020"
                     /inference="COORDINATES: protein motif:HMM:NF012700.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DNA mismatch repair protein MutS"
                     /protein_id="HRV99910.1"
                     /translation="LFATHYHELTCLDERYPGIENLSMAVKEDSDGIFFLHQVVKGSA
                     DRSYGIEVARLAGLPRPVLKRAFELLQRFEKDEKCAAPPQHAAKRKEIPGIRQMTLFE
                     SARSGIVEEIAALTPDTLTPFRALELLYSLVEKSREAIRSENLSSS"
     gene            453..>1160
                     /gene="mutL"
                     /locus_tag="P5201_15025"
     CDS             453..>1160
                     /gene="mutL"
                     /locus_tag="P5201_15025"
                     /inference="COORDINATES: protein motif:HMM:TIGR00585.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006298 - mismatch repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair endonuclease MutL"
                     /protein_id="HRV99911.1"
                     /translation="MYSRIAAGEVVERPVSVVKETVENALDAGATEISVALHEGGKLR
                     IVIEDNGEGMSFDDLPLAVARHATSKIRTVEELETVRSLGFRGEALASIAVVSRLEIR
                     SRKAGEEQGALLSIEGGRTVRHVPTPCRPGTRISVEDLFYTLPARRKFLKTAAAEARR
                     VSALVRDLAVAYPAVAFLEQHDGKQVFSSPGDGDRESLLRAMWGDPRQAPRTSSGRAL
                     DAGFSGAELRMCEAAATN"
BASE COUNT          204 a          348 c          403 g          205 t
ORIGIN      
        1 gctcttcgcg acgcactacc acgagttgac ctgcctcgac gagcgctatc cggggatcga
       61 gaacctgagc atggccgtga aggaggattc ggacggcatc ttctttctgc atcaggtggt
      121 gaaaggaagc gcggaccggt cgtacggtat cgaagtcgcg cgtcttgcgg gattgcctcg
      181 ccctgtgctc aagagggcgt tcgagctgtt gcagcgcttc gagaaggatg agaaatgcgc
      241 cgcgccgccg cagcacgccg ccaaacggaa ggagatcccc ggaatccggc agatgacgct
      301 gttcgagtcc gcgcgctccg gcatcgtcga ggagatcgcg gcgctgacgc cggatacgct
      361 gacacccttc cgcgcgctgg aattactcta ttcgctggtc gagaaaagca gggaggcgat
      421 tcgctctgaa aatctctctt cttcctgagg acgtgtactc ccgcatcgcg gcgggagagg
      481 tggtcgaacg ccccgtttcc gtggtgaagg agacggtgga gaacgcgctt gacgcgggag
      541 cgacggagat ttccgtagct ctccacgagg gcggaaagct ccgtatcgtc atcgaggaca
      601 acggcgaggg aatgtcgttc gacgatctgc ctctggccgt agcccgccac gcgacgagca
      661 aaatacgcac cgtcgaggag ctggagaccg tccgctcgct cggattccgg ggggaggcgc
      721 tggcgagcat cgccgtcgtg agccgcctgg agatccgttc gcggaaagcc ggggaggagc
      781 agggcgcgtt gctctcgatc gagggagggc gcactgttcg ccatgtgccg acgccctgcc
      841 gtccggggac gcgcatctca gtcgaggatc ttttctacac gctcccggcg cgcagaaaat
      901 tcctgaaaac tgcggcggcg gaggcgcggc gagtttcggc gctggtgcgc gatctcgccg
      961 tggcgtaccc cgccgtcgct tttttggaac agcacgacgg caagcaggtc ttctcctctc
     1021 cgggggacgg cgacagagag tcgctgcttc gcgccatgtg gggggatccc cgtcaggcgc
     1081 cgcgtacttc ctccggcaga gcgctcgacg ccgggttctc gggcgcggaa ctccgtatgt
     1141 gcgaggcggc ggcgacgaat
//
LOCUS       DAOTIL010000983         1159 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminobacteriaceae bacterium isolate AMR_MDS_3026
            NODE_147657_length_1159_cov_3.449275, whole genome shotgun
            sequence.
ACCESSION   DAOTIL010000983 DAOTIL010000000
VERSION     DAOTIL010000983.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868050
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminobacteriaceae bacterium (wastewater metagenome)
  ORGANISM  Aminobacteriaceae bacterium
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae.
REFERENCE   1  (bases 1 to 1159)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1159)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:19:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,224
            CDSs (total)                      :: 3,198
            Genes (coding)                    :: 3,184
            CDSs (with protein)               :: 3,184
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1159
                     /organism="Aminobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_147657_length_1159_cov_3.449275"
                     /isolate="AMR_MDS_3026"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:3034609"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..738)
                     /locus_tag="P5201_15030"
     CDS             complement(<1..738)
                     /locus_tag="P5201_15030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005659495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-4-keto-pentose/UDP-xylose
                     synthase"
                     /protein_id="HRV99912.1"
                     /translation="MNILILGANGFIGSHLIKGVLESTDWRINAFDLSGTNLEQYRDS
                     THFSFTQGDIFTADEWLKKAVEAADVVLPLAGIAKPSYYLSKPLWTFELDFEQNLKIV
                     RMCVAADKKVIFPSTSEVYGMSTDTVLKEEESPLIVGPINKMRWIYSCGKQMMDRVIV
                     AYGQEKGLRYTLFRPFNWIGPRLDTFKDAEERTARSITQMVYDVLSGRGITLVNGGEQ
                     MRSFTHISDGVAALMAILADEKASNGKI"
     gene            complement(814..>1159)
                     /locus_tag="P5201_15035"
     CDS             complement(814..>1159)
                     /locus_tag="P5201_15035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006299978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="formyltransferase"
                     /protein_id="HRV99913.1"
                     /translation="EATYFGRRTPADGMIDWQESAVRIHNLVRAVTHPFPGAFTFLRG
                     KKLFIWKTQTGEHPSFDTPPGTVLSEDPLRIFTGRGVLRLLEWQFEDSRAQSPGAGDI
                     LGKEPEGETRPS"
BASE COUNT          208 a          335 c          365 g          251 t
ORIGIN      
        1 gatcttcccg ttgctcgctt tttcgtcggc gagtatggcc atcagcgccg cgacgccgtc
       61 ggagatgtgc gtgaagctcc gcatctgctc tccgccgttg acaagggtga tcccccgtcc
      121 ggagagcacg tcgtagacca tctgcgtgat ggagcgggcg gtgcgctcct cggcgtcctt
      181 gaaggtgtcg aggcgggggc cgatccagtt gaacgggcgg aagagcgtgt agcgcagccc
      241 cttctcctgc ccgtaggcca cgatgaccct gtccatcatc tgcttgccgc agctgtagat
      301 ccagcgcatc ttgttgatcg ggccgacgat gagcgggctt tcttcctcct tgaggacggt
      361 gtcggtgctc atgccgtaga cttccgaagt ggagggaaag atgacttttt tatccgcggc
      421 gacgcacatg cggacgatct tgaggttctg ctcgaagtcg agttcaaagg tccacaatgg
      481 tttgctgagg tagtaggagg gctttgcgat gcccgcgagc ggcaggacga cgtcggctgc
      541 ttccactgcc tttttcagcc attcgtccgc ggtgaagatg tcgccctgcg taaaggagaa
      601 gtgcgtgctg tcccggtact gctcaaggtt cgtccccgaa agatcgaaag cgttgatccg
      661 ccagtccgta ctctccagga ctcccttgat gagatgggag ccgatgaagc cgttggcccc
      721 gagaatcaga atgttcatag agaaacctcc ctgcgtgttc cccgcgttgt gcgggatggt
      781 tcgttcgttg aaaaaagtcg ccacgagagt atatcacgaa gggcgcgttt cgccctccgg
      841 ctctttgccc agaatatctc ctgcgcccgg tgattgcgcc cgggagtcct cgaactgcca
      901 ttcgaggaga cgaagaacgc cccgtcctgt gaaaatgcgg agcggatcct ccgagagtac
      961 cgttcccgga ggcgtatcga aagacggatg ctccccggtc tgcgtcttcc agatgaagag
     1021 cttcttgccc cggagaaagg tgaaggcgcc ggggaaggga tgcgtcaccg cgcggaccag
     1081 gttgtgaatc cggacggcgc tctcctgcca atcgatcatg ccgtccgcgg gcgtcctgcg
     1141 gccgaagtat gtcgcctct
//
LOCUS       DAOTIL010000984         1151 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminobacteriaceae bacterium isolate AMR_MDS_3026
            NODE_149247_length_1151_cov_3.275547, whole genome shotgun
            sequence.
ACCESSION   DAOTIL010000984 DAOTIL010000000
VERSION     DAOTIL010000984.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868050
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminobacteriaceae bacterium (wastewater metagenome)
  ORGANISM  Aminobacteriaceae bacterium
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae.
REFERENCE   1  (bases 1 to 1151)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1151)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:19:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,224
            CDSs (total)                      :: 3,198
            Genes (coding)                    :: 3,184
            CDSs (with protein)               :: 3,184
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1151
                     /organism="Aminobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_149247_length_1151_cov_3.275547"
                     /isolate="AMR_MDS_3026"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:3034609"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            68..1105
                     /locus_tag="P5201_15040"
     CDS             68..1105
                     /locus_tag="P5201_15040"
                     /inference="COORDINATES: protein motif:HMM:NF012652.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase, T2SS/T4P/T4SS family"
                     /protein_id="HRV99914.1"
                     /translation="MDAQEDRTKSSTESGKDRHPHAGAGKISLKEALGGETTDVHLFP
                     AVSSTPEDAPLSGAKARVRIGGVLGPLTAVDADVDGELLALLNNPPKPDFSTGLPSGR
                     RLRVAIRTVCSGIAREIFIRVLPETIPPVFGKLAHLLPELASVTPGLVLVSGITGSGK
                     STLLASVAMRHLELGAHVVTVEDPVEYLLHSKTTTGYASQREVGLDVPDFASGVKMSL
                     REDPDTIIVGEIRDRETALATLTAAETGHAVFATVHAGGCFGTVDRFLSLVGGNEFEA
                     MRLAGVYLCGIHVTSNPAEEKQGLFVDRTYEIFRSNDAARTHIRDRSAHRLWQARLGT
                     SEKDWKLTIRS"
BASE COUNT          219 a          324 c          398 g          210 t
ORIGIN      
        1 gaaagcggtg aaaagttggt actgctaatg gaccgtatat gtcgaacaaa aaacaaggtg
       61 aacgccgatg gatgcacagg aagatagaac gaagagcagt accgaaagcg ggaaagatcg
      121 ccatcctcat gcaggcgcag ggaaaatttc gctgaaagag gcgctcggcg gggagacgac
      181 ggatgtccat ctcttccctg cggtgtcttc tacgcccgag gacgctccgc tcagcggagc
      241 gaaggcgagg gtgcgcatcg ggggcgtgct ggggcccctg acggcggtgg acgcggatgt
      301 cgacggcgag ctgctcgcgc tgctcaacaa cccgccgaaa cccgacttct cgaccggtct
      361 gccgtccgga aggcggctcc gcgtggcgat tcgtacagta tgttccggta tcgcgaggga
      421 gatcttcatc cgcgtgctgc cggagactat ccccccggtg ttcggcaagc ttgcccacct
      481 gcttccggaa ctcgcttcgg tgactcccgg gctggtgctg gtctcgggga tcacggggag
      541 cggcaagtcg acgctgctcg cgtccgtggc catgcggcat ctggagcttg gggcgcacgt
      601 ggtgacggtg gaggatccgg tggaatacct tctccactcg aaaacaacca cggggtacgc
      661 gtcgcagcgg gaggtcgggc tcgacgttcc ggacttcgcc tccggggtga agatgtccct
      721 gcgcgaagat cctgacacga tcatcgtcgg ggagattcga gacagggaga cggcgctcgc
      781 cactctcacc gcggcggaga cggggcacgc tgtatttgcc acggtgcacg cggggggctg
      841 tttcggcacg gtggaccgct tcctctcgct cgtcggagga aacgaatttg aggccatgcg
      901 ccttgcaggg gtgtatctct gcggtattca tgtgacgtcg aatcctgcag aggagaagca
      961 agggctgttc gttgaccgga cctacgagat cttccgcagc aacgacgccg cgcggacgca
     1021 tatccgggac cggtcggccc atcgcctctg gcaggcgcgg ctggggacct cggagaagga
     1081 ctggaagctg acgatccgga gctgatatgg cttttcggag tctgttttcc ggtggcaatc
     1141 acgacagatg g
//
LOCUS       DAOTIL010000985         1150 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminobacteriaceae bacterium isolate AMR_MDS_3026
            NODE_149419_length_1150_cov_3.587215, whole genome shotgun
            sequence.
ACCESSION   DAOTIL010000985 DAOTIL010000000
VERSION     DAOTIL010000985.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868050
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminobacteriaceae bacterium (wastewater metagenome)
  ORGANISM  Aminobacteriaceae bacterium
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae.
REFERENCE   1  (bases 1 to 1150)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1150)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:19:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,224
            CDSs (total)                      :: 3,198
            Genes (coding)                    :: 3,184
            CDSs (with protein)               :: 3,184
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1150
                     /organism="Aminobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_149419_length_1150_cov_3.587215"
                     /isolate="AMR_MDS_3026"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:3034609"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..350)
                     /locus_tag="P5201_15045"
     CDS             complement(<1..350)
                     /locus_tag="P5201_15045"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRV99915.1"
                     /translation="MRRGLFDEACLPLETVLLFIAGMTVFIAGILLFPVYAGVAPYYE
                     NGLYGLLLFFFALQTVMLGKTPFGELPVSKPLLAVGVTIASIGIVTCFIPGIPNKLAR
                     WMLVLCLGPGGALLL"
     gene            complement(387..>1150)
                     /locus_tag="P5201_15050"
     CDS             complement(387..>1150)
                     /locus_tag="P5201_15050"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013047879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HRV99916.1"
                     /translation="ASGIGLGLVEHLLAKGAAGVFMGDVKEENLTRESKRLNEKYPGK
                     VFPVLTDVTKQEQVAELVSKAKAFDGHLDFVFNNAGVGLTLPTEKVTFEMWKFVVDLN
                     LMGVVYGTYSAIPVMREQGFGHIVNTGSITGRVPVPYQAVYAATKAAVISMTESLQFE
                     LETEGLNFSVFCPGNVATAIFGDVPPPPDAIGVDDAVEYILAEVEKKSLVIILPQQMR
                     DFDAVYREHREEYDAFARKMAAERRENYRTKGTYF"
BASE COUNT          214 a          368 c          355 g          213 t
ORIGIN      
        1 agcaggagcg ctcctccggg gccgaggcag agaacaagca tccagcgggc gagcttgttc
       61 gggatgccgg ggatgaagca ggtcacgatc ccgatggagg cgatcgtcac gccgaccgcg
      121 agcagcggtt tcgacacggg aagctctccg aacggcgtct tcccgagcat gacggtctgc
      181 agggcgaaga agaagagcag aagtccgtac agcccgttct catagtacgg cgccacgccc
      241 gcgtacacgg ggaagagcag gattccggcg atgaacaccg tcatcccggc gatgaacagc
      301 aacacggtct ccagcggaag acacgcttcg tcaaacagtc cgcggcgcat cggatcgcgc
      361 ctcctttcct tcgtcggcgc gcgcgcctaa aagtacgttc cctttgtcct gtagttctcc
      421 cggcgctcgg cggccatctt ccgggcgaag gcgtcgtact cctccctgtg ttctctatag
      481 accgcgtcaa aatcgcgcat ctgctgcggg aggatgatca cgagcgattt cttctccacc
      541 tccgcgagga tgtactccac cgcgtcgtcc acgccgatag cgtcgggagg cggcggaacg
      601 tcgccgaaga tcgccgtggc gacgttgccg gggcagaaga cgctgaagtt gagcccctcc
      661 gtctccagct cgaactgcaa gctctccgtc atggagatca cggcggcttt cgttgcggcg
      721 tagactgcct ggtacggaac gggaacccgc cccgtgatcg agccggtatt gacgatatgt
      781 ccgaagccct gctcgcgcat gacggggatc gcgctgtacg tgccgtagac gacgcccatc
      841 aggttgaggt cgacgacgaa cttccacatc tcgaaggtga ccttctccgt gggaagcgtc
      901 aagcccactc cggcgttgtt gaagacgaag tcgagatgtc cgtcgaacgc tttcgccttg
      961 gagacgagct cggcgacctg ttcctgcttc gtcacgtcgg tgagcacggg gaagaccttt
     1021 cccgggtact tctcgttcag gcgcttcgac tcccgcgtca ggttctcttc ctttacgtcg
     1081 cccatgaaga cgccggcggc gcccttcgcc agcagatgct cgacgagccc caggccgatt
     1141 cccgaagcgg
//
LOCUS       DAOTIL010000986         1149 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminobacteriaceae bacterium isolate AMR_MDS_3026
            NODE_149650_length_1149_cov_2.687386, whole genome shotgun
            sequence.
ACCESSION   DAOTIL010000986 DAOTIL010000000
VERSION     DAOTIL010000986.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868050
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminobacteriaceae bacterium (wastewater metagenome)
  ORGANISM  Aminobacteriaceae bacterium
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae.
REFERENCE   1  (bases 1 to 1149)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1149)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:19:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,224
            CDSs (total)                      :: 3,198
            Genes (coding)                    :: 3,184
            CDSs (with protein)               :: 3,184
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1149
                     /organism="Aminobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_149650_length_1149_cov_2.687386"
                     /isolate="AMR_MDS_3026"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:3034609"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..319)
                     /locus_tag="P5201_15055"
     CDS             complement(<1..319)
                     /locus_tag="P5201_15055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009164971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma factor-like helix-turn-helix DNA-binding
                     protein"
                     /protein_id="HRV99917.1"
                     /translation="MRTTKEETVLAKRVYLHDLYDLYAPLLTEKQRKAWDLHEFADFS
                     LAETAEKLGISRQGVSDLITRSRDRLEELESLLGFFHKEDAFEEEIRELRRRIEHLSS
                     GRGG"
     gene            437..1138
                     /locus_tag="P5201_15060"
     CDS             437..1138
                     /locus_tag="P5201_15060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005661539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactate utilization protein"
                     /protein_id="HRV99918.1"
                     /translation="MDENSKRTFPLVEAMKLTNQFDTARQEHRDRLGGTLVKRLEERG
                     FHALYVQTKEEALKEVGKLIPEGASVGVPGSVTIREIGAMEDLAKRGCTVIHHWNPSL
                     SAEERMDTLQNELLADYFLTSSNAITLDGLLVNIDGNGNRVSGMAWGKNTLIFVIGLN
                     KVTNSLDEAIARTRNQATPPNALRLKLQTPCASTGRCSDCNSPDRACKALLVLERATG
                     GRTSHVILVGEDLGF"
BASE COUNT          301 a          307 c          296 g          245 t
ORIGIN      
        1 ccccgccccg gcccgaagaa agatgctcaa tacggcgccg aagctcgcgg atttcttctt
       61 caaacgcgtc ttctttgtga aaaaaaccaa gaaggctttc cagctcctcc agtcgatcac
      121 gacttcgcgt aataagatcg gacacgccct gacggctgat tccaagtttt tcggctgtct
      181 cggcaagaga gaaatccgca aactcatgga gatcccaagc cttacgttgc ttttcagtta
      241 acaatggagc ataaaggtcg tacaagtcgt ggagatacac tctcttggct aaaactgttt
      301 cttctttcgt cgtgcgcatc tcgcagcctc ccctggaact gtggaattat agcccccttc
      361 gcaaaaagtg tcaagctttt tctcttgaca gcattctata ttccggagcg aaacgttcgg
      421 agagaaaaat gatatgatgg acgaaaattc aaagcggaca ttcccgcttg tggaggcgat
      481 gaaattgacg aatcagttcg atacggcccg acaggaacac cgcgaccggc tgggcggtac
      541 attggtaaaa aggctggaag aaagaggctt tcacgctctc tatgttcaga caaaagaaga
      601 ggccctcaaa gaggttggaa agctcatccc ggagggcgcc tccgtcggcg tccccggcag
      661 cgtcacaatt cgtgaaatcg gcgccatgga agaccttgcg aagagaggat gcaccgtaat
      721 ccatcactgg aacccgtccc tgtctgcgga agaacgcatg gacacgcttc aaaacgagct
      781 cttggccgat tacttcctta caagctcgaa tgccatcact ctcgacggat tgctggtcaa
      841 tatcgacgga aacggaaaca gggtaagcgg catggcgtgg gggaaaaaca cgcttatctt
      901 cgttattgga ctgaacaaag tgacgaacag tttggacgag gcgattgcac gaacgcgcaa
      961 ccaggctact cccccgaatg ctcttcggct aaaattgcag acgccgtgcg ccagcacagg
     1021 acgttgctcg gactgcaatt ctcccgacag ggcctgtaaa gcgctcctcg ttcttgagcg
     1081 cgcaaccggc gggagaacgt cgcatgtcat ccttgtcgga gaggacctgg gcttttaaaa
     1141 ccgaacaaa
//
LOCUS       DAOTIL010000987         1147 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminobacteriaceae bacterium isolate AMR_MDS_3026
            NODE_150017_length_1147_cov_4.579670, whole genome shotgun
            sequence.
ACCESSION   DAOTIL010000987 DAOTIL010000000
VERSION     DAOTIL010000987.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868050
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminobacteriaceae bacterium (wastewater metagenome)
  ORGANISM  Aminobacteriaceae bacterium
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae.
REFERENCE   1  (bases 1 to 1147)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1147)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:19:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,224
            CDSs (total)                      :: 3,198
            Genes (coding)                    :: 3,184
            CDSs (with protein)               :: 3,184
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1147
                     /organism="Aminobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_150017_length_1147_cov_4.579670"
                     /isolate="AMR_MDS_3026"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:3034609"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..364
                     /locus_tag="P5201_15065"
     CDS             <1..364
                     /locus_tag="P5201_15065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="stage II sporulation protein SpoIID"
                     /protein_id="HRV99919.1"
                     /translation="PPGTGVPTSLTPMSAAEERQLTDLTEQGVFNAEELMDMLLNPEK
                     RKGYLIRALRTVRPGIPVQPQPQPVGTASGRFLFRGKGWGHGVGLSQWGAKALADQGW
                     DFKKILSFYFPGTKLEGM"
     gene            374..>1147
                     /locus_tag="P5201_15070"
     CDS             374..>1147
                     /locus_tag="P5201_15070"
                     /inference="COORDINATES: protein motif:HMM:NF014592.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-adenosylmethionine:tRNA
                     ribosyltransferase-isomerase"
                     /protein_id="HRV99920.1"
                     /translation="MKTVDLFDIESYNYMLPEELIAQNPATPRDSSRMLVLDRFSGKM
                     MHRRFSDLRELLKPSDLLVLNDTRVIPARLRGSKLPGGGKAEVLLLKALNPEWSEWEA
                     LVKPGRRLRSGTGIILSDGTAITVGERFEGTEGVRRVSFPQDVKVLSLLTEIGETPLP
                     PYITRSTAPRSAYQTVFAARDGSAAAPTASLHFTEELLEELRREIGLSVAWVTLHVGL
                     GTFRPVQCSDIRKHVIHEEYCVLPLETKKLVEETNRHGGR"
BASE COUNT          246 a          294 c          347 g          260 t
ORIGIN      
        1 tcccccagga acgggcgtcc ccacgtcgct gacgccgatg agcgcggcgg aagaacgaca
       61 actgaccgat cttaccgagc aaggggtttt caacgctgag gagctgatgg atatgttgct
      121 caaccctgaa aagcgaaagg ggtaccttat ccgtgcactg cgtactgtac gtcccggtat
      181 ccctgtacag ccccaaccgc aacccgtcgg tacggcttcc ggcaggtttc ttttccgggg
      241 caaggggtgg gggcatggcg tcggactctc ccagtggggg gcgaaggcgc ttgcagacca
      301 agggtgggat tttaaaaaga ttctttcgtt ctactttccc gggacgaaac ttgaagggat
      361 gtagaccgcg tttatgaaga ccgtcgatct tttcgacata gagtcgtaca attatatgtt
      421 accggaggaa ctcattgcac agaatccagc aacacccagg gattcatcgc gaatgctggt
      481 attggaccgt ttttccggaa agatgatgca ccgtcgtttt tccgatctac gggagctgct
      541 gaagccatcc gatctcttgg tcctcaatga cacccgcgtt attcctgcgc gtctgcgagg
      601 aagcaagctt cccggaggag ggaaggcaga ggtgctcctt ctgaaagcgc tcaacccgga
      661 gtggagcgag tgggaggccc tcgtcaagcc tggccggagg cttcgatccg gaacgggaat
      721 catactctcg gatggaacag cgattactgt tggcgaacgt tttgaaggta cggaaggggt
      781 gcggagagtc tcttttcctc aagacgtcaa ggtactttca ttgttgacgg aaatcgggga
      841 gacgcctctt cctccgtata ttacccgctc tacagcgcct cgttcggcat atcagacggt
      901 cttcgccgca cgggacggtt ccgcggcggc gccgactgcg agtctccatt tcaccgagga
      961 gcttctggaa gagctcaggc gggagatcgg tctttcggtc gcctgggtga cgctgcatgt
     1021 cgggctcggg acatttcgtc cggttcagtg ttcggatatt cgtaagcatg ttattcacga
     1081 ggagtattgc gtgcttcctc tagagacgaa aaaacttgtg gaggagacaa atagacatgg
     1141 aggcagg
//
LOCUS       DAOTIL010000988         1146 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminobacteriaceae bacterium isolate AMR_MDS_3026
            NODE_150362_length_1146_cov_2.046746, whole genome shotgun
            sequence.
ACCESSION   DAOTIL010000988 DAOTIL010000000
VERSION     DAOTIL010000988.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868050
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminobacteriaceae bacterium (wastewater metagenome)
  ORGANISM  Aminobacteriaceae bacterium
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae.
REFERENCE   1  (bases 1 to 1146)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1146)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:19:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,224
            CDSs (total)                      :: 3,198
            Genes (coding)                    :: 3,184
            CDSs (with protein)               :: 3,184
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1146
                     /organism="Aminobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_150362_length_1146_cov_2.046746"
                     /isolate="AMR_MDS_3026"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:3034609"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1146)
                     /locus_tag="P5201_15075"
     CDS             complement(<1..>1146)
                     /locus_tag="P5201_15075"
                     /inference="COORDINATES: protein motif:HMM:NF024964.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4135 domain-containing protein"
                     /protein_id="HRV99921.1"
                     /translation="IRTIAAGACFLREKSAYCSAVASESATPEPWNPERRELLERWIR
                     HAGGNGGKSAFLTRLAAEGIEERTLQCLLSSENGAGFEEPLPEWAVRLQKILEPSSSE
                     EEGASIPADSEPLFTALKPFLLFAARALREHLGKEREDTCLPASMHDELLAGLAKRLM
                     RIGEKTYRAEIHRRLAVAYPLQIILGRPPAQEITTTEAKRLAADLRTGGWFDLLRTYP
                     VLGRRLSETTGDWVTYTVELLDRLKKDCYELEQVFSISPSIAALSGMETDASDPHNGG
                     RTVAVLRWKTGERLVYKPRSGGIDQAWRRWIAWVESAGLPCCPVPAALDKGDYCWVEY
                     LTPRPLSDKEDAELFYHRSGVLLCIFYVLGANDFHMENMIAQGSYPVP"
BASE COUNT          232 a          352 c          327 g          235 t
ORIGIN      
        1 cggcacaggg tacgaacctt gggcgatcat gttctccata tggaagtcgt tggctcccaa
       61 tacgtagaat atgcacaata aaactccgga tcgatgataa aagagttcgg cgtcctcctt
      121 gtccgacagg gggcgcggag taagatattc gacccagcag taatcgcctt tgtccagcgc
      181 cgccggaacc ggacagcacg gcaaccccgc cgactcgacc catgcgatcc agcgacgcca
      241 agcctgatct attcctccgc ttcgcggttt gtagaccaga cgctctcccg tcttccagcg
      301 aagaactgcc acggtccggc ctccgttgtg agggtcggac gcatccgtct ccatcccgct
      361 cagtgcggcg atcgacgggg aaatgctgaa gacctgctcc agctcgtagc aatctttttt
      421 caatcgatcc aggagttcga ccgtatatgt cacccaatct cccgttgtct cggaaagacg
      481 tcggccgagc accggataag tccggagcag atcgaaccag ccccccgttc gcaggtcggc
      541 cgccagccgc ttggcttcgg ttgtggtaat ctcctgcgcc ggcggacggc cgagaatgat
      601 ctgcaacggg taggcaactg caagtctgcg gtggatttcg gcgcgatagg ttttctcgcc
      661 gatccgcatc aatcgtttcg ccaatccggc caagagttcg tcgtgcatgg aagcggggag
      721 acaggtatcc tccctttctt tcccgagatg ctcccgtagg gcgcgcgctg caaacagaag
      781 aaagggcttc aaggccgtga aaagaggctc ggaatcggca ggaatcgaag cgccctcctc
      841 ttccgaagac gacggctcga ggattttctg cagccggacc gcccattcgg ggagcggttc
      901 ttcgaatccg gcgccgttct ccgaagaaag aaggcattga agcgttcgct cttcaatgcc
      961 ttccgccgca agacgagtga gaaaggcgct tttgccgccg tttcctcccg cgtgccgaat
     1021 ccatcgttcg agcagttcac ggcgctcggg attccatggc tcgggcgttg cgctttcaga
     1081 ggcgacagcg ctgcaatacg ccgatttttc gcgcaggaaa cacgctcccg ccgctatcgt
     1141 gcggat
//
LOCUS       DAOTIL010000989         1143 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminobacteriaceae bacterium isolate AMR_MDS_3026
            NODE_150916_length_1143_cov_2.856618, whole genome shotgun
            sequence.
ACCESSION   DAOTIL010000989 DAOTIL010000000
VERSION     DAOTIL010000989.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868050
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminobacteriaceae bacterium (wastewater metagenome)
  ORGANISM  Aminobacteriaceae bacterium
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae.
REFERENCE   1  (bases 1 to 1143)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1143)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:19:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,224
            CDSs (total)                      :: 3,198
            Genes (coding)                    :: 3,184
            CDSs (with protein)               :: 3,184
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1143
                     /organism="Aminobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_150916_length_1143_cov_2.856618"
                     /isolate="AMR_MDS_3026"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:3034609"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>1143
                     /locus_tag="P5201_15080"
     CDS             <1..>1143
                     /locus_tag="P5201_15080"
                     /inference="COORDINATES: protein motif:HMM:NF014758.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HRV99922.1"
                     /translation="PVVLSRGVLHAWSAYRLPNARIVGRALATNTAPNGAFRGFGAPQ
                     TVFAMERQMDRIARELGLSPLEVRRKNLLKTGDTLPCGQVLRSAYAGLVLERALDISG
                     YEEKHGKQQEDDRILRGVGVSVFLHGGGFTGAGEEKISGRAAVEVADDGIVEVLVSST
                     EMGQGAATVLPQIAAEVLSLPAWKVRHPNPDTSRVPDSGPTVASRTTMMVGKILQDAA
                     KDMRATLTGEAAKNAGVLPESILWEHGRLASGGRDLGSFEELALQYKEEHGKPLRGEG
                     VYNPPSDCQWNEAEYRGDAYKDYAWACDVVEVEIDADTLELRVPHVTSVVEIGKAVHP
                     MLAEGQVIGGTLQALGWGAMEEVRMEKGRYRNNSVSTYIIPTAVDAP"
BASE COUNT          231 a          320 c          382 g          210 t
ORIGIN      
        1 ccggtggttc tctcacgagg cgtgctccat gcgtggagcg cctacaggct tcccaatgcc
       61 cgtattgtcg gcagggcgct ggcgacgaat accgccccca acggagcgtt ccgcggtttc
      121 ggcgctccgc agactgtttt tgccatggaa cgtcagatgg acaggatagc ccgcgagctt
      181 gggctctctc cgcttgaagt gcgacggaaa aaccttttga aaacaggaga tacgctcccg
      241 tgcggccagg tgctgcgttc ggcctatgcc ggccttgtgc tcgaaagggc gctcgacata
      301 tccggatacg aggaaaaaca tggaaagcag caagaggatg accgcattct gcgcggggta
      361 ggcgtctccg tctttctgca cggcggagga ttcaccgggg cgggggagga gaaaatctcc
      421 ggccgcgccg ctgtggaagt agccgacgac ggaattgtcg aggtgctcgt cagcagcacc
      481 gagatgggcc agggcgccgc gacggtgctt ccgcagatag cggcggaagt cctctctctt
      541 ccagcctgga aggtgcggca tccgaatccc gatacttcgc gcgtacccga cagcggcccg
      601 acagtcgctt cacgaacgac catgatggtt gggaaaatac ttcaggacgc cgcgaaggat
      661 atgcgcgcca cgcttaccgg cgaggcggcg aaaaatgccg gagtacttcc tgaatccatc
      721 ctctgggagc acggacgcct tgcttccggg gggagagatc tgggctcttt tgaggaactt
      781 gccttgcagt acaaggagga gcacgggaag cccctgagag gggaaggagt gtataatccc
      841 ccctccgact gtcagtggaa cgaggccgag taccgcggcg acgcatacaa ggattacgcc
      901 tgggcctgcg atgtggtgga agtggagatc gacgcggata cgctggagct tcgcgtcccg
      961 cacgttacct ccgttgttga aatcggcaag gcggtgcacc ctatgcttgc tgaagggcag
     1021 gttatcggcg ggacgcttca ggcgcttggc tggggcgcga tggaggaggt ccgaatggag
     1081 aagggacgct accgcaacaa tagcgtgtcg acctacatta ttcccacggc cgtggatgcg
     1141 cct
//
LOCUS       DAOTIL010000990         1143 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminobacteriaceae bacterium isolate AMR_MDS_3026
            NODE_150938_length_1143_cov_2.545037, whole genome shotgun
            sequence.
ACCESSION   DAOTIL010000990 DAOTIL010000000
VERSION     DAOTIL010000990.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868050
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminobacteriaceae bacterium (wastewater metagenome)
  ORGANISM  Aminobacteriaceae bacterium
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae.
REFERENCE   1  (bases 1 to 1143)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1143)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:19:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,224
            CDSs (total)                      :: 3,198
            Genes (coding)                    :: 3,184
            CDSs (with protein)               :: 3,184
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1143
                     /organism="Aminobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_150938_length_1143_cov_2.545037"
                     /isolate="AMR_MDS_3026"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:3034609"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..482
                     /locus_tag="P5201_15085"
     CDS             <1..482
                     /locus_tag="P5201_15085"
                     /inference="COORDINATES: protein motif:HMM:NF016200.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="TRAP transporter small permease"
                     /protein_id="HRV99923.1"
                     /translation="ERVDALVGRMERWMLILLLTAMVALGLLQIISRFVIKAPITWSE
                     ALLMYMFVWFSFIGASLAVKELAHFEVEIFMVRLPQPLQKTVAVVVYCLIFAFALFMV
                     WKGVFLVKLNQRQLMAMMPFTMSVPYVILPLSGACMAIHSLKHVVGLIGRHSSKWSD"
     gene            484..>1143
                     /locus_tag="P5201_15090"
     CDS             484..>1143
                     /locus_tag="P5201_15090"
                     /inference="COORDINATES: protein motif:HMM:NF018513.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter large permease subunit"
                     /protein_id="HRV99924.1"
                     /translation="MAATIFAVFTLLLVIRMPVSFSLAISAAVALVFAGSMDPLIVVQ
                     RMYTSVESFSLIAIPFFILSGGFMEHGGISRRLISFSSSLVGHIRGGLSMISIVAAMF
                     FAGISGSTAADTAAVGSVLIPAMEKKGYGKDLATSVVATAGAIGIIIPPSIPMIILGI
                     TGSISIGGLFLGGVFPGILMGLALMVTSSLFAKNRNLPSEVRATWTERRHAFTDSILA
                     LM"
BASE COUNT          182 a          348 c          329 g          284 t
ORIGIN      
        1 tggaacgtgt agacgctctt gtaggacgca tggaacgctg gatgttgatc cttcttctga
       61 cggcgatggt ggcgctgggg cttctccaga tcatcagccg gttcgtcatc aaagcgccga
      121 tcacgtggtc ggaggccctc ctcatgtaca tgttcgtctg gttcagcttc atcggcgcaa
      181 gtctcgccgt gaaggagctg gcgcacttcg aggtcgagat tttcatggtc cgtcttccgc
      241 agccgctgca gaagacggtc gccgttgtcg tgtactgttt gatcttcgcc tttgccctct
      301 tcatggtctg gaagggggtt tttctggtga agctcaacca gaggcagctc atggcgatga
      361 tgccctttac gatgagcgtc ccctacgtga tactgccgct gagcggcgcc tgcatggcga
      421 ttcattcgct gaaacacgtc gtcggcctta tcggccgtca ttcttcgaag tggagtgatt
      481 aacatggctg ccactatttt cgcggttttc acgctgctgc tcgtcatccg tatgccggtc
      541 tccttttcgc ttgccatttc ggcggccgtt gccctcgtct ttgcggggag catggacccg
      601 ctcatcgtcg ttcagagaat gtacacttcg gtggagtcgt tctccctgat cgccatcccg
      661 ttcttcattc tctcgggagg atttatggag catggcggga tctcgcgccg cctgatctcc
      721 ttctcgtcga gcctcgtcgg gcatatcagg ggcgggctct ccatgatcag catcgtcgcg
      781 gccatgttct tcgcaggaat ctccggctct accgcggcag acaccgccgc cgtgggcagc
      841 gtgctcatcc ccgcgatgga gaaaaaaggg tacggcaagg atctggcgac cagcgtggtg
      901 gcgacggcgg gggccatcgg catcatcatc cccccgagca tcccgatgat tatcctcggc
      961 attacgggga gcatctccat cggcgggctc ttcctcggcg gagtcttccc cggcatcctg
     1021 atggggctcg ccctgatggt caccagttcg ctcttcgcca aaaaccgcaa ccttccttcc
     1081 gaagtacgcg ccacatggac ggaacgccgg catgcgttca ccgactccat tcttgccctg
     1141 atg
//
LOCUS       DAOTIL010000991         1142 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminobacteriaceae bacterium isolate AMR_MDS_3026
            NODE_151127_length_1142_cov_2.777369, whole genome shotgun
            sequence.
ACCESSION   DAOTIL010000991 DAOTIL010000000
VERSION     DAOTIL010000991.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868050
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminobacteriaceae bacterium (wastewater metagenome)
  ORGANISM  Aminobacteriaceae bacterium
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae.
REFERENCE   1  (bases 1 to 1142)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1142)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:19:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,224
            CDSs (total)                      :: 3,198
            Genes (coding)                    :: 3,184
            CDSs (with protein)               :: 3,184
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1142
                     /organism="Aminobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_151127_length_1142_cov_2.777369"
                     /isolate="AMR_MDS_3026"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:3034609"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..334)
                     /locus_tag="P5201_15095"
     CDS             complement(<1..334)
                     /locus_tag="P5201_15095"
                     /inference="COORDINATES: protein motif:HMM:NF027862.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar assembly protein T N-terminal
                     domain-containing protein"
                     /protein_id="HRV99925.1"
                     /translation="MRIRNFFFVFLMILLSAAPAFSAGVQAAGELIKVEAEGFAPIKG
                     GDKNGAREAAKRSAYRDALEKALGAYVTGITEMQNFEVVKDKVFSQTTGIVKKFDIVR
                     EKVGDDGIL"
     gene            complement(338..>1142)
                     /locus_tag="P5201_15100"
     CDS             complement(338..>1142)
                     /locus_tag="P5201_15100"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRV99926.1"
                     /translation="MMIVFLPGSISFATEVEIELHPITTEKEKGLIDWTQNHIEATGM
                     AVAPKNMKGAQAKALARRGAIVDLQRNLLEFMGGVQVDARTTMDDFMAEDRVRSEVHG
                     MIKNVELLKGEWDGEAYTVTGRIKLAEVRSIVCPRVPVDTIIIETERKTPAPKASGKY
                     TGLVIDVRHLPLIPSVTFRVFDESGRAVYGVEFANQASMLQSGICSYYNNINYAQGEL
                     RVAPNPVKTKAIRLSTGNVDIVIPNSAAAKIRGSSFDFRKECKVLLVVK"
BASE COUNT          223 a          338 c          313 g          268 t
ORIGIN      
        1 agagtatccc gtcgtccccg accttctccc tgacgatatc gaacttcttg acgattcccg
       61 tcgtctgaga aaagaccttg tccttgacca cctcgaagtt ctgcatctcg gtgattccgg
      121 taacatacgc tccaagagcc ttttcaagcg catccctgta ggcggaacgc ttggcggctt
      181 ccctcgcgcc gttcttgtcg ccgcccttta tcggagcgaa cccctcggct tcgactttga
      241 taagctcccc cgcggcctga actcctgcgg agaacgcagg agcggccgac aagaggatca
      301 tcagaaaaac aaaaaagaaa ttccgtatac gcatgtccta tttcacgaca aggagcactt
      361 tacactcttt gcggaagtcg aacgagctgc cgcggatctt cgccgccgcc gagttcggga
      421 tgacgatatc gacgttcccg gtcgaaaggc gaatcgcttt ggtcttgacg ggatttggag
      481 cgacgcggag ttcgccctgg gcgtagttga tgttgttgta gtaggagcag attcccgact
      541 ggagcatcga ggcctggttc gcgaactcga cgccgtagac ggctctgccg ctttcgtcga
      601 agacacggaa ggtcaccgaa ggaatcagcg gaaggtgccg tacgtcgatg acgaggccgg
      661 tgtactttcc ggaagccttg ggcgcgggcg tctttcgttc ggtctcgata atgattgtat
      721 cgacgggaac cctggggcat acgatggaac gcacttcggc gagtttgatc cgcccggtaa
      781 cggtataggc ctccccgtcc cactcgccct tgagaagctc cacattcttg atcatgccgt
      841 gcacttcgga acggactctg tcctcggcca tgaaatcgtc catggtcgtc ctcgcgtcga
      901 cctgcactcc gcccatgaac tcgagcagat tccgctgaag gtcgacgatc gcgccgcgtc
      961 gggccagtgc cttcgcctgt gcgcccttca tgtttttcgg tgcgaccgcc attcccgtgg
     1021 cttcgatatg gttctgcgtc cagtcgataa gccctttttc cttttccgtg gtgatggggt
     1081 gcagctcaat ttcaacttcc gtcgcgaatg atatgctgcc gggaagaaac acaatcatca
     1141 ta
//
LOCUS       DAOTIL010000992         1141 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminobacteriaceae bacterium isolate AMR_MDS_3026
            NODE_151291_length_1141_cov_3.423573, whole genome shotgun
            sequence.
ACCESSION   DAOTIL010000992 DAOTIL010000000
VERSION     DAOTIL010000992.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868050
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminobacteriaceae bacterium (wastewater metagenome)
  ORGANISM  Aminobacteriaceae bacterium
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae.
REFERENCE   1  (bases 1 to 1141)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1141)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:19:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,224
            CDSs (total)                      :: 3,198
            Genes (coding)                    :: 3,184
            CDSs (with protein)               :: 3,184
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1141
                     /organism="Aminobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_151291_length_1141_cov_3.423573"
                     /isolate="AMR_MDS_3026"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:3034609"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..113)
                     /locus_tag="P5201_15105"
     CDS             complement(<1..113)
                     /locus_tag="P5201_15105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012322358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide ABC transporter ATP-binding
                     protein"
                     /protein_id="HRV99927.1"
                     /translation="MLSARGLSKAYKGRTVVSSADLDVAMGEVVGLLGPNGA"
     gene            complement(119..862)
                     /gene="lptC"
                     /locus_tag="P5201_15110"
     CDS             complement(119..862)
                     /gene="lptC"
                     /locus_tag="P5201_15110"
                     /inference="COORDINATES: protein motif:HMM:NF018537.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter periplasmic protein
                     LptC"
                     /protein_id="HRV99928.1"
                     /translation="MKLFRCIGALLALLLCLGAASAREVKLTADTMQYDSASGYFTAT
                     ENVKIVREGVTATAKKAEGNMNGGEVNLIGDVHIFGVWEDEKVDIKGQILTGYLQDPR
                     GLSFKGTVQGVWGSKQVDAEDLSTRGDTFSVAKLRRFADSAEGFSFSCNSIKGKLKDG
                     EIVEFTAVGAFRFVSSQKKGTPTEIRGDRAVYSKARGSLVVNGNVSAVQDGRSLRSDS
                     LVYFPVKNRIEATGKPQLIFKIDEGSKKQ"
     gene            complement(940..>1141)
                     /locus_tag="P5201_15115"
     CDS             complement(940..>1141)
                     /locus_tag="P5201_15115"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="aspartate kinase"
                     /protein_id="HRV99929.1"
                     /translation="TEVAKVALKGEHLAGCPEIPSQMFSVLAGCRINIDMIAAASTVI
                     ACIVAAGDLEGAVGALSQAFLH"
BASE COUNT          221 a          370 c          305 g          245 t
ORIGIN      
        1 gccccgtttg gtccgagcag tcccaccact tcgcccattg cgacgtccag gtctgccgag
       61 gagacgaccg tcctgccttt ataggctttg gacaggccgc gagccgagag cattttgctc
      121 attgtttctt tgaaccttcg tctattttga agatcagctg cggcttcccc gtggcttcga
      181 tgcggttctt tacaggaaag tagaccagcg aatcggagcg gagcgaacgc ccgtcctgga
      241 cggccgagac gttcccgttc accacgagcg acccccttgc cttggagtag accgccctgt
      301 cgccgcggat ctccgtcggc gttccttttt tttgggacga gacgaatctg aaggcgccga
      361 cggcggtaaa ctcgacgatc tccccgtctt tgagcttccc cttgatcgag ttgcacgaaa
      421 aggagaaccc ctcggcggaa tcggcgaaac ggcgaagttt cgctacggag aacgtatccc
      481 cgcgcgtgct cagatcctcg gcgtccacct gtttgctccc ccagacgccc tgcacggttc
      541 ctttaaagga aagaccgcgc ggatcctgga ggtatccggt caggatctgc ccctttatat
      601 cgactttttc gtcttcccac actccgaaga tatgaacatc gccgataaga ttgacttctc
      661 cgccgttcat gttcccctcg gcttttttgg cggtcgcagt gacgccctcc cggacgatct
      721 tcacattttc ggtcgccgta aagtacccgc ttgcggaatc gtactgcatg gtatccgccg
      781 tcaacttcac ctcacgggcg gaggcggcgc cgaggcagag cagcagcgca aggagcgcgc
      841 cgatacatcg gaacagtttc atgaggctcc ctcccacaca caaaatttcc cggacagcct
      901 tgatatttta cagcacttcg tccgtacgga acagcgtaat cagtgcagaa aggcctgaga
      961 gagcgcgccg accgctcctt ccagatcgcc cgccgcaacg atgcacgcaa tgacggtgga
     1021 cgccgccgcg atcatgtcga tgttgatcct gcaccccgcg agcaccgaga acatctgcga
     1081 ggggatctcc ggacacccgg caagatgctc tcccttgagc gccaccttcg cgacctccgt
     1141 g
//
LOCUS       DAOTIL010000993         1140 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminobacteriaceae bacterium isolate AMR_MDS_3026
            NODE_151595_length_1140_cov_2.023963, whole genome shotgun
            sequence.
ACCESSION   DAOTIL010000993 DAOTIL010000000
VERSION     DAOTIL010000993.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868050
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminobacteriaceae bacterium (wastewater metagenome)
  ORGANISM  Aminobacteriaceae bacterium
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae.
REFERENCE   1  (bases 1 to 1140)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1140)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:19:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,224
            CDSs (total)                      :: 3,198
            Genes (coding)                    :: 3,184
            CDSs (with protein)               :: 3,184
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1140
                     /organism="Aminobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_151595_length_1140_cov_2.023963"
                     /isolate="AMR_MDS_3026"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:3034609"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(45..>1140)
                     /locus_tag="P5201_15120"
     CDS             complement(45..>1140)
                     /locus_tag="P5201_15120"
                     /inference="COORDINATES: protein motif:HMM:NF024700.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HRV99930.1"
                     /translation="EKLILKGYKTVRELSNFELRDLSVLIGPNGAGKSNFISFFRMLA
                     RMCRTPGELQFHVGESGGASMLLHDGPSKTREMEARLLFSGESAGAEYAFRLAFAADD
                     TFVFAEEGAVLFDADGAEVLEWRDSLSGRREPQLLARSDGDASARGIADFLRGIRVYQ
                     FHDTSSTSRMRAKWDVEDGGELKEDGANLAPFLYRLRRDEPRYYRRITETIRLVLPFF
                     ADFVLEPEGERLLLKWSESGCDKVFSASQAADGMLRAVALVTLLLRPERELPRIVILD
                     EPELGLHPYAITIVGGLIRAISKKSQVIVATQSASLVDCFEPDDIVVVERKGRESLFG
                     RLDEDSLGEWLENYSISELWEKNVIGGRPS"
BASE COUNT          243 a          362 c          319 g          216 t
ORIGIN      
        1 ggtttcctca gtctgccctt cgacgacgaa gcagaggcgg acgctcacga cgggcgccct
       61 ccgatcacgt tcttctccca cagctcggaa atcgagtaat tctccagcca ctctccgagc
      121 gaatcttcgt ccaatcttcc gaagagtgac tcccttccct ttcgttccac cacgacaata
      181 tcgtcgggtt cgaaacagtc gacgagggag gcggactgcg tcgcgacgat cacctgcgac
      241 ttctttgaaa tcgcccgtat cagccctccg acaatggtga tcgcgtacgg atgaaggccg
      301 agttcgggtt cgtccagaat cacgatccgg ggaagctctc gctcggggcg cagaagcaaa
      361 gtcacgagcg ccactgcgcg cagcatgccg tcggccgcct gcgacgcgct gaacaccttg
      421 tcgcaccccg actcgctcca cttcaaaaga aggcgttctc cctccggctc cagcacgaaa
      481 tccgcgaaaa aggggaggac gagtcggatc gtttcggtaa tccggcggta gtagcggggc
      541 tcgtcccgtc gcagacggta gaggaagggg gcgaggttgg cgccgtcctc cttgagttct
      601 cctccgtcct cgacatccca cttggcgcgc attcttgagg tggaagacgt atcgtgaaac
      661 tgatagacgc gtatgccgcg cagaaagtcc gcaatgccgc gagcggaagc gtccccatcc
      721 gagcgcgcga gaagctgcgg ctcccttcgg ccggagagag aatcccgcca ctccaagact
      781 tccgctccgt ccgcatcaaa gagaacagcg ccttcttccg cgaaaacgaa tgtatcgtcg
      841 gcggcgaacg cgaggcgaaa cgcatactcg gcgccggccg attcgccgga gaaaagaagc
      901 cgcgcttcca tctcccttgt ttttgacggc ccgtcgtgca aaagcatgct cgctccgccc
      961 gattcgccca cgtggaactg aagctcgccc ggagttctgc acatacgggc aagcatccgg
     1021 aaaaaggaga tgaagttcga tttgccggct ccgttcggcc cgatgagaac cgacagatcg
     1081 cgaagctcga aattcgaaag ctcccggacg gttttataac ctttcaatat cagtttttcc
//
LOCUS       DAOTIL010000994         1139 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminobacteriaceae bacterium isolate AMR_MDS_3026
            NODE_151677_length_1139_cov_4.333948, whole genome shotgun
            sequence.
ACCESSION   DAOTIL010000994 DAOTIL010000000
VERSION     DAOTIL010000994.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868050
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminobacteriaceae bacterium (wastewater metagenome)
  ORGANISM  Aminobacteriaceae bacterium
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae.
REFERENCE   1  (bases 1 to 1139)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1139)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:19:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,224
            CDSs (total)                      :: 3,198
            Genes (coding)                    :: 3,184
            CDSs (with protein)               :: 3,184
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1139
                     /organism="Aminobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_151677_length_1139_cov_4.333948"
                     /isolate="AMR_MDS_3026"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:3034609"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..974)
                     /locus_tag="P5201_15125"
     CDS             complement(<1..974)
                     /locus_tag="P5201_15125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013048111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004826 - phenylalanine-tRNA ligase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase beta subunit-related
                     protein"
                     /protein_id="HRV99931.1"
                     /translation="MLISWNLLNEILTIPASLEEVAERLTFTGCEVESIEYPCKSLKD
                     VRSARIEKLEKHPAKENLFVAVVDDGKGSAVVVTAAPNLSAGDIVPWGRPGAVLADGS
                     VLGTRDFEGVESVGMLLSAAELGIPEAADEFGILRLPAETAPGEDVKHLLGLDDAILD
                     LSVTPNRGDLLSMLGVAREVYALFPEATWKMDLTAFPANAMNAEWPVDFKGITLKDDG
                     CPLYCLGLITGLKSVPSPLRTRILLTLLGMRPISAMVDATNYAMLMLGQPTHAFDADR
                     LPSPEITVRSAADGEETRTLDGKNHRLEPGDMLITSGGLAVGIAGVMGG"
     gene            complement(968..>1139)
                     /gene="pheS"
                     /locus_tag="P5201_15130"
     CDS             complement(968..>1139)
                     /gene="pheS"
                     /locus_tag="P5201_15130"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013048110.1"
                     /note="catalyzes a two-step reaction, first charging a
                     phenylalanine molecule by linking its carboxyl group to
                     the alpha-phosphate of ATP, followed by transfer of the
                     aminoacyl-adenylate to its tRNA; forms a heterotetramer of
                     alpha(2)beta(2); binds two magnesium ions per tetramer;
                     type 1 subfamily; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit alpha"
                     /protein_id="HRV99932.1"
                     /translation="HPNVIRSGGIDPDVYNSFAWGIGLDRIAMLKYRLTDLRVLFEGN
                     VPYLLSGRRFTC"
BASE COUNT          241 a          349 c          328 g          221 t
ORIGIN      
        1 cctcccatca cgccggcgat gccaacggca agaccgccgc tcgtgatgag catatcgccg
       61 ggttcaagcc ggtggttttt cccgtcgagc gtcctggttt cttcaccgtc ggcggcggag
      121 cgaacggtaa tctccggcga cggaagacgg tcggcgtcaa aggcgtgcgt cggctgaccg
      181 agcatgagca tggcgtagtt ggtcgcatcg accattgcgg agatcggacg cattccaagc
      241 agcgtgagga gaatgcgcgt ccgcaacggc gagggaacgc ttttcaggcc cgtgatcagg
      301 ccgaggcagt agagcgggca tccgtcgtct ttcaaggtaa tccccttgaa atcaaccggc
      361 cactcggcat tcatggcgtt cgctgggaaa gctgtaagat ccattttcca cgtggcctcg
      421 gggaagagcg cgtacacttc gcgcgccact ccgagcatgc tcagcagatc tcccctgttc
      481 ggcgtcaccg agaggtcgag gattgcgtcg tcaagcccca gaagatgttt gacatcctct
      541 ccgggcgcgg tctccgcagg caaacggaga atgccgaatt catcggccgc ctccggtatg
      601 cccagttccg cggccgagag cagcatcccc acgctctcga cgccttcgaa atccctcgtg
      661 ccgagaacgg aaccgtcggc gagcacagcg cccggacgcc cccatggaac aatatcccct
      721 gcggaaaggt tcggcgccgc cgttacgacg accgctgagc ccttcccgtc gtccacaacg
      781 gctacgaaga ggttttcttt cgccgggtgt ttttcaagct tctcgatacg cgcagagcgc
      841 acatccttca aagatttgca tggatattct atcgattcga cttcgcaacc cgtgaaagtc
      901 aaccgttccg cgacctcttc aagcgacgcg ggaatcgtca gaatttcgtt cagcagattc
      961 caggaaatca gcatgtgaaa cgcctccccg agagaagata gggcacgttg ccttcaaaga
     1021 gaacgcgcaa atcggtcagc cggtatttga gcatggcgat gcggtcaagg ccgattcccc
     1081 aggcaaaact gttgtacaca tccggatcga tgcctccgga acggataacg ttcggatgc
//
LOCUS       DAOTIL010000995         1137 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminobacteriaceae bacterium isolate AMR_MDS_3026
            NODE_152276_length_1137_cov_1.746765, whole genome shotgun
            sequence.
ACCESSION   DAOTIL010000995 DAOTIL010000000
VERSION     DAOTIL010000995.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868050
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminobacteriaceae bacterium (wastewater metagenome)
  ORGANISM  Aminobacteriaceae bacterium
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae.
REFERENCE   1  (bases 1 to 1137)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1137)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:19:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,224
            CDSs (total)                      :: 3,198
            Genes (coding)                    :: 3,184
            CDSs (with protein)               :: 3,184
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1137
                     /organism="Aminobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_152276_length_1137_cov_1.746765"
                     /isolate="AMR_MDS_3026"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:3034609"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            84..530
                     /locus_tag="P5201_15135"
     CDS             84..530
                     /locus_tag="P5201_15135"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRV99933.1"
                     /translation="MGKSIQILFVFLFLLALLTMCTILEAASETVQNEPVAIPGEYLV
                     LFKARESISELEGRDRKIAVTIMLEMQGEYLTTKFGVIVQNTFSAISESSGKGMFFVR
                     SDNAAKDPEFSKKLLEEMRSDPFIEGVSPNEVKKMISRPGIGTETP"
     gene            756..>1137
                     /locus_tag="P5201_15140"
     CDS             756..>1137
                     /locus_tag="P5201_15140"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase-like regulatory
                     domain-containing protein"
                     /protein_id="HRV99934.1"
                     /translation="MRIPLLALLFFVLMLPVAAATVVEGADSPLVEGMVTSEGKGVFG
                     IEINIWDGQTNRKAVSDSEGRFSIAGLEPGTTFALRSTPRNHRSVRADGFRFPEKGNL
                     YLSMEYAETQAGQRHSIRVPSNPST"
BASE COUNT          305 a          243 c          298 g          291 t
ORIGIN      
        1 aaaatgcaaa aaatacgtga gtatgctatt atgtaccgga atagagatgg tgcgactact
       61 cgctgaaggg gtgagaagag cagatgggca agagtatcca gatattgttt gtatttttat
      121 ttttactggc gctactgaca atgtgcacaa tcctcgaggc tgcttcggaa acggttcaaa
      181 acgaaccggt agcgataccg ggagagtatc tggttctctt caaagcccgt gaaagcatct
      241 ctgaactcga agggcgagat cgaaaaatcg ctgtgacaat aatgctcgaa atgcaaggag
      301 agtatttgac gacgaagttc ggtgtcatcg tgcagaatac attcagcgca atttcagagt
      361 ccagcggtaa gggaatgttt ttcgttcgta gcgacaatgc ggcaaaggat ccggagttca
      421 gtaaaaaact acttgaagag atgaggtccg acccgtttat tgagggcgtt tcacccaacg
      481 aggttaagaa gatgatatca cggcctggga tcggaaccga gacgccttga atgctctcat
      541 tttctagcta acagcatgtt cttaattcct attttccttt tttaaaggaa aataggaacg
      601 tttgcaacat tcttcagaac tttttctaca aatctatgaa aagaggtggt gcagaataca
      661 gtttttatga atagaagtat gtgtccgatt cctgtcgctt ctgctggacc tcgctcactt
      721 cgaggcgaca catctcgaaa aggtggtgaa gagacatgcg aatacctctt ttagcgttgc
      781 tattcttcgt gcttatgctc cctgttgcgg cggctactgt tgtggaggga gctgattctc
      841 cgcttgttga aggaatggtg acaagcgagg ggaagggtgt tttcggcatc gaaatcaaca
      901 tatgggacgg tcagaccaac aggaaagcag tttccgacag cgaaggcaga ttttcaatag
      961 ctggactcga gccgggaaca acgtttgcgc ttcgctccac cccgcgcaat catcgatccg
     1021 ttcgcgcgga cggattccgc tttcccgaga aggggaatct ctatttgagc atggagtacg
     1081 ccgagactca ggcaggacag cggcattcaa tccgcgttcc ttcgaaccct tcgacag
//
LOCUS       DAOTIL010000996         1136 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminobacteriaceae bacterium isolate AMR_MDS_3026
            NODE_152378_length_1136_cov_2.802960, whole genome shotgun
            sequence.
ACCESSION   DAOTIL010000996 DAOTIL010000000
VERSION     DAOTIL010000996.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868050
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminobacteriaceae bacterium (wastewater metagenome)
  ORGANISM  Aminobacteriaceae bacterium
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae.
REFERENCE   1  (bases 1 to 1136)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1136)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:19:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,224
            CDSs (total)                      :: 3,198
            Genes (coding)                    :: 3,184
            CDSs (with protein)               :: 3,184
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1136
                     /organism="Aminobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_152378_length_1136_cov_2.802960"
                     /isolate="AMR_MDS_3026"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:3034609"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..186)
                     /locus_tag="P5201_15145"
     CDS             complement(<1..186)
                     /locus_tag="P5201_15145"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRV99935.1"
                     /translation="MERLEKVILENLRSRPQEECLWWENGWMTSLDLLSFVEKNVQVL
                     KKAGFKKTITKDLENVYS"
     gene            complement(400..>1136)
                     /locus_tag="P5201_15150"
     CDS             complement(400..>1136)
                     /locus_tag="P5201_15150"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRV99936.1"
                     /translation="SFILLVIMSVILAGLSAVIFLLVPPGARTEGFWISFWAILFSVV
                     LSFSYMIFHVVAGKDDFAPVPLLLGLSTTIACYCIFVLGNVLVSHFWLRLPQNAYIAT
                     HIAGFMLLVGGGGAITLLSLTAKETDVSASLKRSRLFVQTTRVASLVDELSLSPFAGQ
                     AAEMIARLRKLKEAIQYSDPMAPDGLEAEELVVIAVGAVEEKARRFLSAATDQERRAF
                     LDEAAPLVEKAFNTLHRRNEEVKESK"
BASE COUNT          287 a          314 c          291 g          244 t
ORIGIN      
        1 agaataaaca ttctccaaat cctttgtaat tgtttttttg aatcctgcct ttttcaagac
       61 ttgaacattc ttctccacaa aactgagcag atccaaggag gtcatccacc cgttctccca
      121 ccagagacac tcttcctgcg gacggctccg caaattctcc agtatgactt tttccagcct
      181 ctccatctgt tcttctcccc tttcggagta tatgaatgtg gcagttcaca gcagtaatga
      241 cggaaggtat atcaccgtag ggtgacctct ctattgtaaa gcaaaaaagc cctttctgca
      301 taaaaaaaac agaaagggct ccacaaaaag acgctacgtt gtgaaaatat acgcttctat
      361 cccgggttct ttccgggaat ctcgatgtgt gcgaaacgac tatttgctct ccttgacctc
      421 ctcgttccgc ctgtgcaggg tgttgaaagc tttctccaca aggggcgcgg cttcgtcgag
      481 gaaggcgcgg cgctcctgat cggtagccgc cgacaaaaaa cggcgggcct tttcttcaac
      541 tgctcccacg gcgatgacga caagttcttc ggcttccaat ccatccggag ccattggatc
      601 gctgtactgg atcgcctcct tgagcttccg caagcgggcg atcatttcgg cggcctgtcc
      661 tgcgaacggg gagaggctga gttcgtcgac aagggaggca actcgcgtcg tctgtacgaa
      721 aagacggctc cgcttgagcg acgcgctcac gtcggtctcc tttgccgtga gcgagagaag
      781 ggtgatggcg cctcctcctc ccaccagcag catgaaaccg gcgatgtgcg tggcgatata
      841 ggcgttctgc ggcaggcgaa gccaaaagtg tgaaacgagc acgttgccca ggacgaaaat
      901 acagtagcag gcaatggtcg tggaaagccc cagcagcagc ggaaccggcg caaagtcgtc
      961 tttccccgcg acgacgtgga agatcatgta ggagaacgag aggacaaccg aaaagaggat
     1021 cgcccagaag gaaatccaaa aaccttccgt tcgcgctccg ggaggcacaa gcagaaaaat
     1081 gaccgccgag agccccgcga ggatcaccga cattatgaca agcaaaataa aacttt
//
LOCUS       DAOTIL010000997         1134 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminobacteriaceae bacterium isolate AMR_MDS_3026
            NODE_152724_length_1134_cov_3.329935, whole genome shotgun
            sequence.
ACCESSION   DAOTIL010000997 DAOTIL010000000
VERSION     DAOTIL010000997.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868050
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminobacteriaceae bacterium (wastewater metagenome)
  ORGANISM  Aminobacteriaceae bacterium
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae.
REFERENCE   1  (bases 1 to 1134)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1134)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:19:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,224
            CDSs (total)                      :: 3,198
            Genes (coding)                    :: 3,184
            CDSs (with protein)               :: 3,184
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1134
                     /organism="Aminobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_152724_length_1134_cov_3.329935"
                     /isolate="AMR_MDS_3026"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:3034609"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..202)
                     /locus_tag="P5201_15155"
     CDS             complement(<1..202)
                     /locus_tag="P5201_15155"
                     /EC_number="2.3.3.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014162312.1"
                     /note="catalyzes the formation of 2-isopropylmalate from
                     acetyl-CoA and 2-oxoisovalerate in leucine biosynthesis;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-isopropylmalate synthase"
                     /protein_id="HRV99937.1"
                     /translation="MNDAHVRIFDTTLRDGEQAAGINLNKEEKLQIAMQLAKMKTDII
                     EAGFPAASPGDFEAVKLIAQQVD"
     gene            682..969
                     /locus_tag="P5201_15160"
     CDS             682..969
                     /locus_tag="P5201_15160"
                     /inference="COORDINATES: protein motif:HMM:NF023159.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-hairpin-helix domain-containing protein"
                     /protein_id="HRV99938.1"
                     /translation="MNPEKVNRRTVRRLTDLPNVGPATERDLRLLGVEKPEDLAGRCP
                     FEMYDELCEKTGVRQDPCVIDVFMSVTDFMNGSAPRAWWTFTEERKRKTAR"
BASE COUNT          236 a          320 c          327 g          251 t
ORIGIN      
        1 cgtccacctg ctgcgcgatg agcttgaccg cctcgaagtc gccgggggaa gcggcgggga
       61 accccgcttc gatgatgtcc gtcttcattt tcgccagctg catagcgatc tggagtttct
      121 cctccttgtt caggttgatc cccgccgcct gctctccgtc tctcagggtg gtatcgaaaa
      181 tccgtacgtg tgcgtcgttc ataattctct ctcctttcaa aatcatccgt gaaataaaag
      241 tacaaaaaaa ggccggaacc tctaaaaaag aggttccggc cttggaatgc cgttacgcag
      301 cttaccgggc tgccggcgtt ccaggcggga accggcaacc gaggaggagg cacagggagg
      361 aagcgttcat gatgcgtgaa cgtacttctt tagcgccagt tgcatgcgct ataagtgatc
      421 gacacagctt tttactcgct tcgctcgtgt tcgccggtcg tctacttaac ataactgatt
      481 ccctcctctc ctcatggctc ggtaagattg gtacattttg agggagtacc cctggaatgt
      541 caatactcaa acaaaagaaa aacaaaaggc gtttattctg ataaaaggct ataactttga
      601 ccgggtcgcg tggaaaatcc cggcgtggta gaatgcttcg caaagggcgc tccggcgccc
      661 tttcgaggag aggtgcgtgc gatgaacccc gaaaaagtga atcgtcggac ggtccggcgg
      721 ctgaccgatc tgcccaacgt cggaccggcc acggaaagag atctccgtct tctcggcgtt
      781 gagaagccgg aagaccttgc cggtcgctgt ccgttcgaga tgtacgacga gctctgcgag
      841 aagacgggag tccggcagga cccctgcgtg atcgacgtct ttatgtcggt gaccgacttc
      901 atgaacggtt ccgctccgcg cgcgtggtgg acgttcacgg aggagcggaa gcggaaaacg
      961 gcccgctgac gcggcccgcg cgggattacc tcggcggtat cccgcactcg cacgggacgc
     1021 cggtctggca cttcccgcac ccgtatcgcg ggcggtattt ttctttcgtc agagttaaaa
     1081 aatccgcgca gcggacgtga tccttcccct tttcgatggt gatggcgccg accg
//
LOCUS       DAOTIL010000998         1134 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminobacteriaceae bacterium isolate AMR_MDS_3026
            NODE_152802_length_1134_cov_2.104727, whole genome shotgun
            sequence.
ACCESSION   DAOTIL010000998 DAOTIL010000000
VERSION     DAOTIL010000998.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868050
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminobacteriaceae bacterium (wastewater metagenome)
  ORGANISM  Aminobacteriaceae bacterium
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae.
REFERENCE   1  (bases 1 to 1134)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1134)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:19:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,224
            CDSs (total)                      :: 3,198
            Genes (coding)                    :: 3,184
            CDSs (with protein)               :: 3,184
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1134
                     /organism="Aminobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_152802_length_1134_cov_2.104727"
                     /isolate="AMR_MDS_3026"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:3034609"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..226)
                     /locus_tag="P5201_15165"
     CDS             complement(<1..226)
                     /locus_tag="P5201_15165"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRV99939.1"
                     /translation="MKKGSLDSGQRGQIPVTCCYFCEKRKTVTPQKNLACTHMIRYVP
                     AHVVKQRRAMKQRHIYPDSLLPQSESELQAN"
     gene            534..>1134
                     /locus_tag="P5201_15170"
     CDS             534..>1134
                     /locus_tag="P5201_15170"
                     /inference="COORDINATES: protein motif:HMM:NF013134.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD binding domain-containing protein"
                     /protein_id="HRV99940.1"
                     /translation="MSTVQWYFPDTLDEAISYLGREKTVPHGGGTSLIKSGIASLEGL
                     VDLSRLGLDSLKMEKGILEAGAGMTFGGVAESLANLHPECILGKALGASASTPMRNRI
                     TLGGSASLFPLWSDLIGPMSVLHGVICTEGKNGGTYDLPSWLADRTLQKGSLITSLLF
                     PDPVDWLSSYYRETRVGFDYPAFTVSILARKNNSTIDKVR"
BASE COUNT          273 a          294 c          297 g          270 t
ORIGIN      
        1 tgtttgcctg aagctcgctt tcgctttgcg gcaacaggga gtcaggatag atatgccgct
       61 gcttcatggc gcggcgctgt ttcacgacgt gtgcaggcac gtaccggatc atgtgtgtgc
      121 aggcgagatt tttttgcggc gtcacggttt tcctcttctc gcagaaatag cagcatgtca
      181 cagggatctg ccccctttgc cccgaatcga ggctaccctt cttcatcttg cggacaaact
      241 cgtttccgat acgcacatca tttctctcga tgccaggaaa aagaacatgc gtatgcgata
      301 tggtgcagat caaaacgcat tgctggctat ttgccaaaga tacgccgctg caagaaaaac
      361 cgcacgcaga atagaacgca taacaggaca gtctctcgaa actttgctgc aaatttaaga
      421 agaacccctc tagacagttg acattgaatg ttataaagtg aatggatgga ggaacgtctc
      481 tcctccagag tagtgtatga actgtgcaga tgacaaaaca aaggaggaga aatatgagta
      541 ctgtgcaatg gtattttccg gatacactcg atgaagctat ctcgtatctc ggcagggaga
      601 aaactgttcc tcacggcgga ggaacgagtt tgatcaaaag cgggatcgcc tccctggaag
      661 gtctggtaga cctttccaga ttaggccttg attcgctgaa gatggaaaaa ggaatccttg
      721 aagccggagc gggaatgacg ttcgggggcg tcgcggagag ccttgccaat ctgcaccccg
      781 aatgtatcct gggaaaggct ctcggagcgt ctgcctccac cccgatgaga aaccggatta
      841 cgcttggagg cagcgcgtcg ctctttccgc tttggtccga tcttatcggc cccatgtcgg
      901 tgctccacgg agttatctgc accgagggga agaacggtgg gacctacgac ctgccctcct
      961 ggcttgccga cagaactctt cagaagggct ctctgatcac ttcgcttctc ttccctgatc
     1021 cggtcgactg gctctcctcc tactacagag agacccgtgt cggattcgat tatccggcct
     1081 ttaccgtctc tattctcgca cgcaaaaaca acagtacgat agataaggtc cgga
//
LOCUS       DAOTIL010000999         1132 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminobacteriaceae bacterium isolate AMR_MDS_3026
            NODE_153185_length_1132_cov_2.043640, whole genome shotgun
            sequence.
ACCESSION   DAOTIL010000999 DAOTIL010000000
VERSION     DAOTIL010000999.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868050
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminobacteriaceae bacterium (wastewater metagenome)
  ORGANISM  Aminobacteriaceae bacterium
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae.
REFERENCE   1  (bases 1 to 1132)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1132)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:19:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,224
            CDSs (total)                      :: 3,198
            Genes (coding)                    :: 3,184
            CDSs (with protein)               :: 3,184
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1132
                     /organism="Aminobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_153185_length_1132_cov_2.043640"
                     /isolate="AMR_MDS_3026"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:3034609"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..832
                     /locus_tag="P5201_15175"
     CDS             <1..832
                     /locus_tag="P5201_15175"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRV99941.1"
                     /translation="GLVPFAASWGLYAVLGAVIVNRLLIEAEEHGFFAEYIVLFTPFL
                     LIGGGWWFLHWVYRTGGSLFMPLSLPLPEASLFPLTSFVLLIGAAVTLCAIRCGKDRE
                     DCAITFRFFLLCFTYLGIVWLLLPAAETRLRLALLSAGILLLFLAASLLPFRRRLAPF
                     ILLLLLCVVTWRELPAYSQNLGRWSDALRGEGAISGLYPSEESIGRYLHDRLSGIEVG
                     LDGRAPVVEFFAGGRVRFVPPYEQPLPEVVLRPSFTPLSGGDNVLLRGERWTLLQRAR
                     "
     gene            913..>1132
                     /locus_tag="P5201_15180"
     CDS             913..>1132
                     /locus_tag="P5201_15180"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRV99942.1"
                     /translation="MAFPLWRRARCCFVLLLLLCMIFSGTAGAALLRDLESAVRLRSG
                     SGIPVRQSGDGYAVDAALFLLYAVESGNR"
BASE COUNT          136 a          340 c          359 g          297 t
ORIGIN      
        1 cggtctcgtt cccttcgccg cttcgtgggg gctgtatgcc gtcctgggag ccgtgattgt
       61 gaaccggctc ctgatcgaag cggaagagca cggttttttc gccgagtaca tcgttctgtt
      121 cacgcccttc ctgcttatcg gcggagggtg gtggttcctc cactgggtct accggaccgg
      181 gggcagtctc tttatgccgc tctctctgcc tcttccggag gcgtcgctct tccctctcac
      241 ctccttcgtg ctgctgatcg gggcagccgt gacgctctgc gcgatacgct gcggaaagga
      301 ccgggaggat tgcgcgatca ctttccggtt cttcctgctc tgttttacgt atctcgggat
      361 tgtctggctt cttcttcccg ccgcggagac gcggctccgg ctggcgttac tctccgccgg
      421 catcctgctt ctctttcttg ccgcttccct gctgccgttt cgccgccgcc tggcgccgtt
      481 catccttctg ctgcttctct gcgtcgtcac gtggcgggag ctacccgcgt attcgcagaa
      541 tctggggcga tggagcgacg cccttcgagg agagggggcg atctccggcc tgtacccttc
      601 ggaggagagc atcggccgat atctgcatga tcggctctcc ggtatcgagg tcggtctgga
      661 cggacgcgct ccggtggtgg agtttttcgc cggggggagg gtacgctttg tcccgccgta
      721 tgagcagcct cttcctgaag ttgttctgcg gccctccttt acaccgctat ccggcggtga
      781 caacgtgctg ctgcggggcg aacgatggac tcttctgcaa agggcgcgct gaatgtgttc
      841 gcaggtgttt tccctctccg gcggtacggt ttcaaccttg agccgccctt agagcagggg
      901 aggggggccg ctgtggcgtt tcctttatgg agaagagcgc gctgctgttt cgttctcttg
      961 ctgctattgt gcatgatttt ctccggaact gccggagctg ctcttctgcg cgatctggaa
     1021 tcggcggtgc ggcttcggag cggctccgga attcctgtga ggcaaagcgg cgatgggtat
     1081 gctgtggacg ccgcgctctt tcttctctat gcggtcgaat caggaaaccg cg
//
LOCUS       DAOTIL010001000         1131 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminobacteriaceae bacterium isolate AMR_MDS_3026
            NODE_153290_length_1131_cov_3.202602, whole genome shotgun
            sequence.
ACCESSION   DAOTIL010001000 DAOTIL010000000
VERSION     DAOTIL010001000.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868050
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminobacteriaceae bacterium (wastewater metagenome)
  ORGANISM  Aminobacteriaceae bacterium
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae.
REFERENCE   1  (bases 1 to 1131)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1131)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:19:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,224
            CDSs (total)                      :: 3,198
            Genes (coding)                    :: 3,184
            CDSs (with protein)               :: 3,184
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1131
                     /organism="Aminobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_153290_length_1131_cov_3.202602"
                     /isolate="AMR_MDS_3026"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:3034609"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            460..>1131
                     /locus_tag="P5201_15185"
     CDS             460..>1131
                     /locus_tag="P5201_15185"
                     /inference="COORDINATES: protein motif:HMM:TIGR01098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate/phosphite/phosphonate ABC transporter
                     substrate-binding protein"
                     /protein_id="HRV99943.1"
                     /translation="MKTQRVRPVSRSCFRAFLLLLVALCFLRLSSADASENYIFAVYP
                     VHDPNRMLTAFQPLADFIAAETGVPVRLVVTGNYEEMTRRLADGSVHFAFFTSSGYVK
                     HASSIPGLVYLATYAELDSKGGVTPYYHSVILSLEEGPVDALADLKGRNFGFTDKGST
                     SGYKVPLTMLRALGMEPASFFGKVFFLGRHDSVVEALRAGSIQGGAVSDGTLHNAVRQ
                     YGDLFR"
BASE COUNT          229 a          327 c          309 g          266 t
ORIGIN      
        1 tttggagacc acggctccag caagatctcg ttcgtcgtag aaaagcgtca ccgggaccgt
       61 gaacggtctg cgtacataca gaaaacttgc gatggaaagg agtatgatcg ccggtacgac
      121 gatcgtccaa acactttttt tcacaaaata gccctccaag gcggcatgtc gtattgtaga
      181 tccgaaggct ccgaaagttt atttccttat tgtaacaaaa atgcatcgaa cacgcagaga
      241 ttataccaat attttcgtct tgtgaaaatc attttctgtt gacaaatatc ggggcgggac
      301 tttttttgcg cgcctctccg gtcgaaaacg ccttccggag agccttcccg aaactgcgag
      361 acgacggaat gtgcttgacc gcaggagaaa gaggtgctat tttcttgttg agaatctttc
      421 cccgtttgta cgcattcgag agacgacgga ggtggaagta tgaagacgca gcgcgtacgc
      481 cctgtttcac ggagttgttt cagagctttc ctgttgcttc tcgttgctct ctgcttcctg
      541 cggctttcct cggcggacgc atcggaaaac tatattttcg ccgtctaccc cgttcacgac
      601 ccgaacagaa tgcttaccgc cttccagccg ctcgccgact tcatcgccgc ggagacgggc
      661 gttcccgtcc ggctcgtcgt caccggaaac tacgaggaga tgaccaggcg gctcgccgac
      721 ggctccgtcc acttcgcctt cttcacctcc tccggctacg tgaagcacgc atcttccata
      781 ccggggctcg tctacctcgc cacctacgcg gagctggatt cgaaaggggg cgtcacgccg
      841 tactatcatt cggtgattct ctctctcgaa gaggggcctg tcgacgccct cgccgatctg
      901 aaggggcgca acttcggttt taccgacaag ggctcgacct cggggtacaa agttcccctg
      961 acgatgctcc gggcgcttgg aatggagccc gcgtcgttct tcggcaaggt cttcttcctc
     1021 gggaggcacg attcggtcgt cgaggcgctc agggcggggt ccatccaggg aggggccgtg
     1081 tcggacggaa cgctgcacaa cgccgtcagg cagtacggcg atctctttcg c
//
LOCUS       DAOTIL010001001         1131 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminobacteriaceae bacterium isolate AMR_MDS_3026
            NODE_153344_length_1131_cov_2.315056, whole genome shotgun
            sequence.
ACCESSION   DAOTIL010001001 DAOTIL010000000
VERSION     DAOTIL010001001.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868050
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminobacteriaceae bacterium (wastewater metagenome)
  ORGANISM  Aminobacteriaceae bacterium
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae.
REFERENCE   1  (bases 1 to 1131)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1131)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:19:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,224
            CDSs (total)                      :: 3,198
            Genes (coding)                    :: 3,184
            CDSs (with protein)               :: 3,184
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1131
                     /organism="Aminobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_153344_length_1131_cov_2.315056"
                     /isolate="AMR_MDS_3026"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:3034609"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>1131
                     /locus_tag="P5201_15190"
     CDS             <1..>1131
                     /locus_tag="P5201_15190"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRV99944.1"
                     /translation="DVVVLVSKGASVAAEGKYIHQVGAIKVTLEGPEEASWNVNGRGL
                     FASGETASNIPVGEHTIAFSAVQDWDHPAAVPVTVVRDETVEISRAYSKHLGSVSVTI
                     KGPSDARWSLDGIDGMFESGAVRADLPVGEYAVVFKDVANWYAPEKMNVTVVKDEVAQ
                     REASYAEHRGSVIVEIDGPAEARWSLDGEGSYASGESVKDVVVGKHAVSFSDIADWDK
                     PADGTVEVAKDAEASFKGSYAQHKGSLQVVINGPEDARWTLDGKGSYTSGETVKDVVV
                     GTYTLSFSHVNGWDAPQDFQITVAKDTLAKSESGYTRHLGSVTATIEGPADARWSLDG
                     EGSYASGESVDKVVVGDHAVSFSNIADWDTPADQTMSVTKDAA"
BASE COUNT          254 a          287 c          359 g          231 t
ORIGIN      
        1 gacgtagttg ttcttgtttc taagggcgct tccgtcgcag cggaagggaa gtatatccat
       61 caggtcgggg caataaaagt gactcttgag ggtcctgaag aggccagttg gaatgtgaac
      121 ggcagagggc tttttgcgag cggcgagaca gcctcgaaca ttcctgtcgg tgaacacacg
      181 atcgcttttt ccgccgttca ggactgggat catcccgctg ccgtcccggt gacggtggtc
      241 cgtgacgaaa cagtggaaat ctcaagggcg tattccaagc acctcggctc ggtctccgtc
      301 acgatcaagg gaccctccga tgctcgctgg agcctcgatg ggatcgacgg aatgtttgag
      361 agcggcgcag ttcgcgcgga tctcccggtc ggcgagtatg ccgttgtctt caaggacgtt
      421 gcaaactggt acgctccgga gaagatgaac gttaccgttg tcaaagacga ggttgctcaa
      481 cgagaagcct cctacgccga acacagagga tccgtcatcg ttgaaatcga cggtcccgcc
      541 gaagctcgtt ggagcttgga cggcgagggg agctacgcaa gcggagagag cgtaaaagac
      601 gtcgttgtcg ggaagcacgc agtctccttc tccgatattg ccgactggga caagcccgcc
      661 gacggcacag tggaagtcgc caaagacgcc gaagcctcct ttaaaggatc atacgcgcaa
      721 cacaagggct cgttgcaggt agtaatcaac ggtcctgaag atgcgcggtg gaccttggac
      781 ggcaagggga gctacacgag cggtgaaact gtaaaagatg ttgtcgtcgg gacgtacaca
      841 ctctctttct cgcatgtgaa cggttgggat gctcctcagg acttccagat cacagtggcg
      901 aaggatacgc tcgcaaaatc ggagtctggg tatactcggc atcttggctc tgtaacggcg
      961 acgatagaag gtcctgcaga tgcgcgctgg agcttggacg gcgaggggag ctacgcgagc
     1021 ggagagtctg tcgataaagt tgtggtcggg gatcacgctg tttcgttctc caatatcgcc
     1081 gactgggata cgcctgccga tcagacgatg tccgtgacga aagacgctgc g
//
LOCUS       DAOTIL010001002         1130 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminobacteriaceae bacterium isolate AMR_MDS_3026
            NODE_153506_length_1130_cov_2.873488, whole genome shotgun
            sequence.
ACCESSION   DAOTIL010001002 DAOTIL010000000
VERSION     DAOTIL010001002.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868050
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminobacteriaceae bacterium (wastewater metagenome)
  ORGANISM  Aminobacteriaceae bacterium
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae.
REFERENCE   1  (bases 1 to 1130)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1130)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:19:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,224
            CDSs (total)                      :: 3,198
            Genes (coding)                    :: 3,184
            CDSs (with protein)               :: 3,184
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1130
                     /organism="Aminobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_153506_length_1130_cov_2.873488"
                     /isolate="AMR_MDS_3026"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:3034609"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            90..>1130
                     /locus_tag="P5201_15195"
     CDS             90..>1130
                     /locus_tag="P5201_15195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008522200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004427 - inorganic diphosphate
                     phosphatase activity [Evidence IEA];
                     GO_function: GO:0009678 - pyrophosphate hydrolysis-driven
                     proton transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sodium/proton-translocating pyrophosphatase"
                     /protein_id="HRV99945.1"
                     /translation="MLLFLLTLIVAVTGALALLYAYLTYSQKVLFGCTWFKVSEAPEE
                     APEGEMIEKTARVHELSEIIHKGAMAFLYREYKYLLPFLLVVGVLLIYFIGFAAGFSF
                     IIGGLCSAAAGYAGMIVATRTNGKTALAATRGMNEALLTAFRGGSVMGMSVVGIGVLG
                     IVFCYALFRDPNLITGFGMGASTIALFARVGGGIFTKGADVGADLVGKVEAGIPEDDP
                     RNPAVIADNVGDNVGDIAGMGADLFESYVNSIIAAMALGLALFGWKGVSFPMVMAGVG
                     ILAAIVGTRFVKVEEGGNPAVALRKGTFVTGGIMIVGAFIFSFLMFGALSLFWSVLSG
                     VAVGVLIGFVTEI"
BASE COUNT          179 a          328 c          360 g          263 t
ORIGIN      
        1 atgccgtccc taactcgcct cgctgttcga cgaccggacg gaaaaaagat ggttttttca
       61 tatttttcct attgggagga gtctgtgaaa tgctgctttt cttgttgaca ctcattgtcg
      121 cggtcacggg agctcttgcg ttgctctacg cctacctgac gtacagccag aaggtattgt
      181 tcggatgcac ctggttcaag gtgtctgagg cgcccgagga agcgcccgag ggagagatga
      241 tcgagaagac tgcgcgggtt catgagctct ccgagatcat ccacaaaggc gcgatggcct
      301 tcttgtacag ggagtataaa taccttctgc cgttccttct ggtggtcggc gtgctcctga
      361 tctacttcat cggtttcgcg gcgggcttct ccttcatcat cggcggtctg tgcagcgcgg
      421 ccgcagggta tgccggaatg atcgtggcca cgaggaccaa cggcaagacg gctcttgccg
      481 ctacgcgcgg gatgaacgaa gccctgctca ccgccttcag aggcggcagc gtcatgggaa
      541 tgtcggtcgt gggcatcggc gtcctcggta tcgtcttctg ctacgcgctc ttccgcgatc
      601 ctaacctgat cactggattc ggcatgggcg ccagtacgat cgcgctcttt gcgcgtgtcg
      661 gcggcggtat cttcaccaag ggtgcggatg tcggcgccga cctcgtcggc aaggtcgagg
      721 cggggattcc cgaggacgac ccccggaacc cggcggtcat cgcggacaac gtcggcgaca
      781 acgtcggcga catcgcgggc atgggcgcgg acctcttcga gtcgtacgtg aactccatca
      841 tcgcggcgat ggcgctcgga ctcgctctct tcggctggaa aggcgtctcc ttcccgatgg
      901 tcatggccgg cgtgggcatt ctggcagcga tcgtcggaac gcgtttcgtc aaggtggagg
      961 aggggggcaa ccccgccgtc gcgctgcgta aggggacctt cgtcacgggc ggcattatga
     1021 tcgtcggcgc gttcatcttc tccttcctta tgttcggcgc tctttcgctc ttctggtcgg
     1081 tgctctccgg agtggcggtg ggcgttctca tcggtttcgt caccgagatc
//
LOCUS       DAOTIL010001003         1130 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminobacteriaceae bacterium isolate AMR_MDS_3026
            NODE_153549_length_1130_cov_2.285581, whole genome shotgun
            sequence.
ACCESSION   DAOTIL010001003 DAOTIL010000000
VERSION     DAOTIL010001003.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868050
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminobacteriaceae bacterium (wastewater metagenome)
  ORGANISM  Aminobacteriaceae bacterium
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae.
REFERENCE   1  (bases 1 to 1130)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1130)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:19:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,224
            CDSs (total)                      :: 3,198
            Genes (coding)                    :: 3,184
            CDSs (with protein)               :: 3,184
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1130
                     /organism="Aminobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_153549_length_1130_cov_2.285581"
                     /isolate="AMR_MDS_3026"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:3034609"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..198)
                     /locus_tag="P5201_15200"
     CDS             complement(<1..198)
                     /locus_tag="P5201_15200"
                     /inference="COORDINATES: protein motif:HMM:NF016885.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA];
                     GO_function: GO:0048029 - monosaccharide binding [Evidence
                     IEA];
                     GO_process: GO:0005996 - monosaccharide metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RbsD/FucU domain-containing protein"
                     /protein_id="HRV99946.1"
                     /translation="MSERKGGRTMKRSGILHPELAFQIASCGHGDLVCIADAGLPIPP
                     ETPRIDLAYARGQPGFFTVLDA"
     gene            549..>1130
                     /locus_tag="P5201_15205"
     CDS             549..>1130
                     /locus_tag="P5201_15205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015555881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004180 - carboxypeptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HRV99947.1"
                     /translation="MLEKIKKLATVYGDEVVAFRREFHMHPERSWEEVETSKKVVEAL
                     KGLGCTIVKTGFGGTESGVVAEMKGGKPGKCVALRADIDALPLQEENKVPYASQTPCV
                     MHACGHDAHTAILLGAAKILAEIRDELPGTVRFIFQPAEECGLRSGAKVMLDEGALDG
                     VDSIGGLHVWSLAPTGTALYRSGPVMAAADAWYL"
BASE COUNT          210 a          316 c          374 g          230 t
ORIGIN      
        1 ggcgtccagc acggtgaaga agccgggttg cccgcgcgca taggccaggt cgatccgtgg
       61 cgtctccggg ggtattggaa gccccgcgtc ggcgatgcac acaaggtcgc cgtggccgca
      121 ggaagcgatc tggaaagcca gttccgggtg aagaatgccg ctgcgtttca tagtgcgtcc
      181 ccccttgcgt tcgctcatgg tgaagcgtgc gggccgtcta tcgcatactt ccgtggcctt
      241 gcgcccgccc gtcgttcact caagagaagc gctgtaattt atcatgctat tgtcatgtat
      301 cgtagcacgg aggcatggtt tttacaacgt gaatcacgcg tcttcgacca gagaaattcg
      361 tgcattgtac ctacttcaga ctgtgatgtg cggaaggtgc gggcgtgcgg aaagatgccg
      421 ctgcacgaca ccccggatgt ttttcagcta tctgtttcga ggggggcttg tgcggtgcgg
      481 aaaagaatga tagtgttttt gcatatctgt ttcgtattct aaaattcgat tgaacgaggt
      541 gatttctttt gcttgaaaaa atcaagaaac tggcaacggt gtacggcgac gaggtcgtcg
      601 cattccgccg cgagttccac atgcatccgg agcgctcctg ggaagaggtc gagaccagca
      661 aaaaggtcgt cgaggcgctg aagggacttg gctgcaccat cgtgaagacg gggttcggcg
      721 gcacggagtc gggggtcgtc gccgagatga agggcggcaa gccgggcaag tgcgtggcgc
      781 tgcgagccga catcgacgcg ctcccgcttc aggaggagaa caaggtcccg tatgcgtcgc
      841 agactccgtg cgtgatgcac gcctgcggcc acgacgcgca caccgccatc ctgctgggtg
      901 cggcgaagat cctcgccgag atccgtgacg agcttcccgg aaccgtccgt ttcatcttcc
      961 agcccgcgga ggagtgcggc ctgcggtcgg gcgcgaaagt catgctcgac gagggcgcgc
     1021 tcgacggcgt cgactccata ggcggtcttc acgtctggtc gctggccccc acgggaacgg
     1081 cgctctaccg ctccggcccg gtgatggcgg ctgcggacgc gtggtacctg
//
LOCUS       DAOTIL010001004         1130 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminobacteriaceae bacterium isolate AMR_MDS_3026
            NODE_153557_length_1130_cov_2.163721, whole genome shotgun
            sequence.
ACCESSION   DAOTIL010001004 DAOTIL010000000
VERSION     DAOTIL010001004.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868050
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminobacteriaceae bacterium (wastewater metagenome)
  ORGANISM  Aminobacteriaceae bacterium
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae.
REFERENCE   1  (bases 1 to 1130)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1130)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:19:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,224
            CDSs (total)                      :: 3,198
            Genes (coding)                    :: 3,184
            CDSs (with protein)               :: 3,184
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1130
                     /organism="Aminobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_153557_length_1130_cov_2.163721"
                     /isolate="AMR_MDS_3026"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:3034609"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..817
                     /locus_tag="P5201_15210"
     CDS             <1..817
                     /locus_tag="P5201_15210"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRV99948.1"
                     /translation="LEERLRTLRNDGKTQEERRTDLTTLALSWDAAKARVREYGDKLK
                     EFGGDPVQALQKQKSILQAKSAELKATEEEYIRESARLETYAARGLYTVLAGLEEKLA
                     QLEEQRGREQRTADSVRLLYDTFTACRSEMAEAVTRTASERATELFARISGAEGGRLV
                     LEDSMKPSGFVPAELDRAVDVDALSGGEKEQLHFAVRMALADCSGREERRLLVLDDTL
                     MATDGIRFPRVLDIIEEAAEELQILILTCQPERFEPLRKALRIDLGALLGRTA"
     gene            861..>1130
                     /locus_tag="P5201_15215"
     CDS             861..>1130
                     /locus_tag="P5201_15215"
                     /inference="COORDINATES: protein motif:HMM:NF024206.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ankyrin repeat domain-containing protein"
                     /protein_id="HRV99949.1"
                     /translation="MSEQNMLERIKAFHTHCTTASPGEIAEALRNGASPDARDEDRMT
                     ALMWAARRNPDPEAVRTLLAAGAELEARDESGLTPLMWAARNKAMT"
BASE COUNT          239 a          344 c          384 g          163 t
ORIGIN      
        1 gctcgaagaa cgcctccgga cgctccggaa cgacgggaag acgcaggaag agcgtcggac
       61 cgacctgacc acgctcgccc tctcctggga tgccgcgaaa gcgcgggtca gggaatacgg
      121 cgacaagctc aaggagttcg gcggcgaccc ggtgcaggcc ctgcaaaagc agaagtcgat
      181 acttcaggcg aagtccgccg agctgaaggc gacggaggag gagtatattc gagaaagcgc
      241 tcgcctggag acctatgccg cgcgcggact gtacaccgtc ctcgcaggac tcgaggagaa
      301 gcttgcgcag cttgaggagc agcgcggaag agaacaacga actgcggatt ccgtccgtct
      361 gctctacgac acgttcacgg cctgccgttc cgaaatggct gaagcagtca cgcggacggc
      421 gtccgaacgg gcgaccgaac tttttgcccg tatctccggc gccgagggag ggcgccttgt
      481 acttgaggat tcgatgaaac cctcggggtt cgtccctgcg gagcttgaca gggccgtcga
      541 cgtcgacgcc ctttccggag gggagaaaga gcagttgcac ttcgccgtcc ggatggcgct
      601 ggccgactgc tcggggaggg aggaacgacg tctcctggta ttggacgaca cgctcatggc
      661 cacggacggc atccgtttcc cgcgcgtcct cgacatcatc gaagaagcgg cggaggagct
      721 gcagatactg atcctcacct gccagcccga gcgtttcgag cccctgagga aggctctcag
      781 gatcgacctc ggggcgctgc tcgggcgaac ggcctgagga accggaacgt ttccatgaac
      841 tcttgagggg aggcggtacc atgagcgaac aaaacatgct ggagcgcata aaggcctttc
      901 acacgcactg cacaaccgcc tctcccgggg agatcgcgga ggcgctacga aacggagcct
      961 caccggacgc ccgcgacgag gacagaatga ccgcgctcat gtgggcggcg cggaggaacc
     1021 cggatccgga ggcggtgcgt acgctgctcg ccgccggagc cgaactggag gcccgcgacg
     1081 aaagcgggct gacgccgctg atgtgggccg cccgaaacaa agccatgacg
//
LOCUS       DAOTIL010001005         1127 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminobacteriaceae bacterium isolate AMR_MDS_3026
            NODE_154109_length_1127_cov_3.513993, whole genome shotgun
            sequence.
ACCESSION   DAOTIL010001005 DAOTIL010000000
VERSION     DAOTIL010001005.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868050
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminobacteriaceae bacterium (wastewater metagenome)
  ORGANISM  Aminobacteriaceae bacterium
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae.
REFERENCE   1  (bases 1 to 1127)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1127)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:19:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,224
            CDSs (total)                      :: 3,198
            Genes (coding)                    :: 3,184
            CDSs (with protein)               :: 3,184
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1127
                     /organism="Aminobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_154109_length_1127_cov_3.513993"
                     /isolate="AMR_MDS_3026"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:3034609"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(22..876)
                     /locus_tag="P5201_15220"
     CDS             complement(22..876)
                     /locus_tag="P5201_15220"
                     /inference="COORDINATES: protein motif:HMM:NF013434.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HRV99950.1"
                     /translation="MTDKTYDIIIVGAGIVGATLARELSAYALEIAVIDKAADIPSGA
                     SRANSAMIHAGYDDKPGSIKADFCAPGNRLYQELAGPLDITLRKCGSYVCGFDAKDLK
                     HLEKLLDQGKRNGVPGVEIITGDALRAKEPHVSAEIAHALWAPSGCIVNNFEAVLAFM
                     DNAQQNGVELFLETELQDIVLSDDRKEVLGIRTNRGFMRTSVVINAAGINSDLVAKMA
                     GDDSFSIEPTRGEYYIMDKNVGNLVGSFFFPCPTDKGKGITVAPTAENNLLIGPTSVV
                     QESRDDGA"
BASE COUNT          216 a          331 c          326 g          254 t
ORIGIN      
        1 gcatcggggc tattgccgga gtcacgcgcc atcgtcacgg ctctcctgga ccacggaggt
       61 ggggccgata aggaggttgt tctccgccgt cggcgcaact gtaattccct tccccttatc
      121 ggtcgggcac gggaagaaaa agctgccgac gaggttgcct acgttcttgt ccatgatgta
      181 gtactcgcct ctcgtcggtt cgatagagaa tgaatcgtcg ccggccatct tcgcgacaag
      241 gtcggagttg atcccggccg cgttgatgac gacggaggta cgcataaagc cgcggttggt
      301 gcgtattcca agaacctctt tccggtcgtc cgagaggacg atgtcctgaa gctctgtttc
      361 gaggaagagc tcgacgccgt tctgctgcgc attgtccata aacgcgagga ccgcttcaaa
      421 gttgttcacg atgcatccgg agggcgccca cagcgcgtgg gcgatctccg cgctgacatg
      481 cggctccttg gcacgcaggg catcgccggt gatgatctcc actccgggga cgccgttgcg
      541 ctttccctgg tcgagaagtt tttcaaggtg cttcaaatct ttggcatcga agccgcagac
      601 gtaggaacca cacttccgga gcgttatatc cagcggaccg gccagttcct gatagagtct
      661 gttgccgggg gcgcaaaaat ccgccttgat ggagcccggc ttgtcgtcgt atccggcgtg
      721 aatcatggcg ctgttggccc tgctcgcccc ggagggaata tccgccgcct tgtcgatgac
      781 agcgatttca agggcatagg cggaaagctc cctggccagg gtagccccga cgatccctgc
      841 accgacgatg atgatatcgt aggttttgtc cgtcaacttc agaacacctc catagtacga
      901 agagggagaa aggtgcgtgc catgggcagc gtacggcctc tctccctctg tcgcgcagtg
      961 cgatttattt cagaagactt ctcaaaatcg gccgctgcgt gtgtccgcag acagcatcaa
     1021 gttcttagag ccaccccttg gacttgtcga tggccttctt ccaccccttg tacatctctt
     1081 cgcgcttgtc tgcgtacatc tgaaccagac gaccaatacg ctcacgg
//
LOCUS       DAOTIL010001006         1127 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminobacteriaceae bacterium isolate AMR_MDS_3026
            NODE_154137_length_1127_cov_2.714552, whole genome shotgun
            sequence.
ACCESSION   DAOTIL010001006 DAOTIL010000000
VERSION     DAOTIL010001006.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868050
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminobacteriaceae bacterium (wastewater metagenome)
  ORGANISM  Aminobacteriaceae bacterium
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae.
REFERENCE   1  (bases 1 to 1127)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1127)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:19:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,224
            CDSs (total)                      :: 3,198
            Genes (coding)                    :: 3,184
            CDSs (with protein)               :: 3,184
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1127
                     /organism="Aminobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_154137_length_1127_cov_2.714552"
                     /isolate="AMR_MDS_3026"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:3034609"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            233..691
                     /locus_tag="P5201_15225"
                     /pseudo
     CDS             233..691
                     /locus_tag="P5201_15225"
                     /inference="COORDINATES: protein motif:HMM:NF023880.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0016407 - acetyltransferase activity
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="sugar O-acetyltransferase"
     gene            677..790
                     /locus_tag="P5201_15230"
                     /pseudo
     CDS             677..790
                     /locus_tag="P5201_15230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007209919.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="2,3,4,5-tetrahydropyridine-2,6-dicarboxylate
                     N-acetyltransferase"
     gene            complement(884..>1127)
                     /locus_tag="P5201_15235"
     CDS             complement(884..>1127)
                     /locus_tag="P5201_15235"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRV99951.1"
                     /translation="ALAPVGEKGVVFGAMNMIAVLSAVVFQWGTGIVINRFPAGQPGV
                     YTAEGYFAAFLLVSCAMAASLLALRSLGKEPLRQEE"
BASE COUNT          242 a          331 c          351 g          203 t
ORIGIN      
        1 aaaggaaaca atctgctact gcttcaacta tacacgaagc gatatcgaag ccgaccttct
       61 gattaacgga cgctcgacga tcctggaacg catcctcgcc gcgaagaagg cgtctggatg
      121 ccgttgcgcg gagctgaacc cttcgggagg ctgatgcgta ggcgacgtcg gccgtgtgct
      181 ggacgagatc cgaaaacgag gagtgtgaac acgaatgacc gaacgggaaa agatgctcgc
      241 aggcgaactg tacgacccat ccgaccgcga gcttgtgcgc ttgagagctc gggcgcgtga
      301 tctgtgcctc gatctgaacg catcgcgcgc gacggaacag aagcttcggc aacttatcct
      361 gcgggaactc ctcgggagcg gcggagagca cgcgactgtg cagccgccct tccactgcga
      421 ctacggcgtg cacattcacc tgggggacaa agtgtacttc aacttcaact gcatcgtcct
      481 cgacgtctgc gaagtgcgca tcggcgactt cacgatgttc ggcccggcag tgcagatata
      541 cacggcgacc catccgatgg acgcacaggc gcggcggggg aaggagttcg gaaaacccgt
      601 ctttatcgga tcggacgtct gggtcggcgg gggcgcgatc atctgccccg gtgcgacgat
      661 cggctcccgc tccgtcatcg gcgccggaag cgtcgtgacg aaggacatcc ccgaaggcgt
      721 cttcgctgcg ggcaacccgt gcagggtggt gcggaagatc ggggaacacg aggacagaga
      781 atcgctttaa tatgcgtgga aatactttcc gcgcaatgaa gagagcgatc gcgatgtgtg
      841 ttgttgcgtc tttacttgtg tgcgttgcgg cggatctttc ggcttattct tcctgtctca
      901 gcggttcttt cccgagcgaa cgcagtgcga gcaggctggc cgccatcgcg cacgaaacga
      961 gcaggaacgc agcaaaatag ccctccgccg tatagacgcc aggctgtccc gccgggaagc
     1021 gattgatcac gatccccgtc ccccactgga agacaaccgc cgagagaacg gcgatcatgt
     1081 tcatcgcgcc gaaaacgacc cctttctccc caaccggggc aagggcg
//
LOCUS       DAOTIL010001007         1125 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminobacteriaceae bacterium isolate AMR_MDS_3026
            NODE_154526_length_1125_cov_4.779439, whole genome shotgun
            sequence.
ACCESSION   DAOTIL010001007 DAOTIL010000000
VERSION     DAOTIL010001007.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868050
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminobacteriaceae bacterium (wastewater metagenome)
  ORGANISM  Aminobacteriaceae bacterium
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae.
REFERENCE   1  (bases 1 to 1125)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1125)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:19:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,224
            CDSs (total)                      :: 3,198
            Genes (coding)                    :: 3,184
            CDSs (with protein)               :: 3,184
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1125
                     /organism="Aminobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_154526_length_1125_cov_4.779439"
                     /isolate="AMR_MDS_3026"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:3034609"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..323
                     /locus_tag="P5201_15240"
     CDS             <1..323
                     /locus_tag="P5201_15240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015556600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF6305 family protein"
                     /protein_id="HRV99952.1"
                     /translation="AKGLGASGITIDQEVDRLHAIMEKAKELNIPVIAAHIEGKARRG
                     KPGSADERSIDAILPYAAHIVVNKDGDLDKKFTRFGEEHSIPVSYLDNAMDLNAFAKT
                     MYAK"
     gene            388..>1125
                     /locus_tag="P5201_15245"
     CDS             388..>1125
                     /locus_tag="P5201_15245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008521713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="citrate transporter"
                     /protein_id="HRV99953.1"
                     /translation="MYAETSFVLAVMVFTFALASWKLKSPELSMVITAVVGALAGGLG
                     FPVRLLVEGTFTYFDVAFTFLTASIFINFYSATGAMDALVRKMVDRFFDRKWILFFLL
                     AVIMLIPGALTGAGSVSMFVVGGMVATVLRFSGIKETRIVAFIYVTSMLAAVAPPINL
                     WVMLMCAQANMPYVGFEFPLLAPIALITVFTVFYLLRGGSPKDKETVLASLPEAHERM
                     NWFRIAAPLLVFVVLILLSKYAAFSTPV"
BASE COUNT          203 a          367 c          317 g          238 t
ORIGIN      
        1 ccgcaaaggg gcttggcgct tcgggcatca cgatcgatca ggaggtcgac cgtctgcacg
       61 ccatcatgga gaaggccaag gagctgaata tcccggtcat cgccgcgcat atcgagggca
      121 aggcccgccg cggcaaaccg ggaagcgccg acgaacgctc catcgacgcc attctgccgt
      181 acgccgcgca tatcgtggtg aacaaggacg gcgatctgga caaaaagttc acccgcttcg
      241 gtgaagagca ctcaattccc gtaagctacc tcgacaacgc gatggacctc aacgcattcg
      301 ccaaaacaat gtacgcaaaa taaaaaacag gaccccgccg tatctttcga cggggtcttt
      361 tcttttctac ggacgggagg tcctgagatg tacgcggaaa catcgtttgt tctggccgtg
      421 atggtgttca ccttcgccct cgcgagctgg aagctgaaat cacccgaact gtcgatggtg
      481 atcaccgccg tcgtcggcgc gctcgccgga gggctgggat tcccggtccg cctcctggtc
      541 gaagggacgt tcacctactt cgacgtggcc ttcacctttc tgacggcctc catcttcatc
      601 aacttctact cggccaccgg cgcgatggac gcgctggtgc gcaagatggt ggaccggttc
      661 ttcgaccgca agtggattct tttctttctg ctcgccgtca ttatgctcat tcccggcgcg
      721 ctgaccggcg cagggagcgt ttcgatgttc gtcgtcggcg gcatggtcgc cacggtgctc
      781 cgcttcagcg gcatcaagga gacgcgcatc gtcgccttca tctacgtcac gtcgatgctg
      841 gccgccgtcg cgccgccgat caacctctgg gtcatgctca tgtgcgcgca ggccaacatg
      901 ccctacgtgg ggttcgagtt ccctcttctg gcccccatcg cgctcatcac ggtcttcaca
      961 gtcttctacc tgctgcgagg aggctccccc aaggacaagg agacggtgct cgcctccctt
     1021 cccgaagcgc acgaacggat gaactggttc cgtatcgccg cgccgctgct ggtcttcgtc
     1081 gtgctcattc tcctctccaa gtacgcggcc ttctcgacgc cggtg
//
LOCUS       DAOTIL010001008         1125 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminobacteriaceae bacterium isolate AMR_MDS_3026
            NODE_154584_length_1125_cov_2.805607, whole genome shotgun
            sequence.
ACCESSION   DAOTIL010001008 DAOTIL010000000
VERSION     DAOTIL010001008.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868050
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminobacteriaceae bacterium (wastewater metagenome)
  ORGANISM  Aminobacteriaceae bacterium
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae.
REFERENCE   1  (bases 1 to 1125)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1125)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:19:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,224
            CDSs (total)                      :: 3,198
            Genes (coding)                    :: 3,184
            CDSs (with protein)               :: 3,184
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1125
                     /organism="Aminobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_154584_length_1125_cov_2.805607"
                     /isolate="AMR_MDS_3026"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:3034609"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>1125
                     /locus_tag="P5201_15250"
     CDS             <1..>1125
                     /locus_tag="P5201_15250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005660652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HRV99954.1"
                     /translation="NSVDEAIGGFCTRIAVTIHENESVEVEDNGRGIPTDPHPSNGRP
                     ASEVVLTTLHAGGKFDGGAYKVSGGLHGVGVSVVNALSEWLEITICRNGESRSQRFEK
                     GIPATDLSEGIPTEHTGTKVHFLADATIFEEVKYSFDVLGARFREMAFLNPGLSITLD
                     DKREGGRVKEYRYEGGMRSFIEYLNRGKTVLFPDPIVISGEKDGVSVDIGLQYNDGYQ
                     ERVFGFANLIHTIEGGTHVSGLRTALTRAVNEAARRAKLLKEKEENLSGDDLKEGLTS
                     VISVKLGNPQFEGQTKTKLGNSDVKGIADSIVYEGLVAWFDDHPQIIKTVVEKAIKAR
                     QAREAAKRARELVRKTAMTGLTLPGKLADCSGRNPENTEV"
BASE COUNT          248 a          349 c          354 g          174 t
ORIGIN      
        1 acaactcggt ggacgaggcg atcggcggct tctgtacccg gatcgcagta accatccacg
       61 agaacgaaag cgtcgaggtc gaggacaacg gccgcggcat ccccacggac cctcatcctt
      121 caaacggccg tccggcctcg gaagtggtgc tcaccacgct ccatgcaggc ggcaagttcg
      181 acggcggcgc atacaaggtc agcggcggcc tccacggggt tggcgtttcg gtagtgaacg
      241 ccctctccga atggcttgaa ataacgatat gccgcaacgg agagtcgcga agccagcgtt
      301 tcgagaaggg gattcccgcg acggatctct ccgagggtat tccaaccgaa cacaccggga
      361 ccaaggtgca cttcctggcc gacgcgacga tctttgaaga ggtgaagtac tccttcgacg
      421 tgctcggcgc tcgattccgc gagatggcct ttttgaaccc gggactctcc atcacgctcg
      481 acgacaagag agagggcgga cgggtcaaag agtaccgcta cgaaggggga atgcgctcct
      541 tcatcgagta cctcaaccgc ggcaagaccg tgctcttccc cgatccgata gtgatttcgg
      601 gcgagaagga cggcgtttcc gtggacatcg gccttcagta caacgacggc taccaggagc
      661 gcgtcttcgg cttcgccaac ctgatccaca cgatcgaggg agggacgcac gtctccggtc
      721 tccggacggc gctcacccgc gcagtgaacg aagccgctcg ccgggccaag ctgctgaagg
      781 agaaggaaga aaacctctcc ggcgacgacc tcaaggaggg actgacctcc gtcatctccg
      841 tgaagcttgg gaacccgcag ttcgaggggc agacgaagac gaagctgggc aacagcgacg
      901 tgaagggaat cgccgactcc atcgtctacg aggggctggt cgcctggttc gacgaccatc
      961 cgcagatcat caagaccgtc gtcgagaagg cgatcaaggc gcggcaggcc cgcgaggccg
     1021 cgaagcgcgc ccgggagctt gtccgcaaga ccgcgatgac gggactgacg ctccccggca
     1081 agctggccga ctgttccggc agaaatcccg agaacaccga agtct
//
LOCUS       DAOTIL010001009         1125 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminobacteriaceae bacterium isolate AMR_MDS_3026
            NODE_154587_length_1125_cov_2.781308, whole genome shotgun
            sequence.
ACCESSION   DAOTIL010001009 DAOTIL010000000
VERSION     DAOTIL010001009.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868050
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminobacteriaceae bacterium (wastewater metagenome)
  ORGANISM  Aminobacteriaceae bacterium
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae.
REFERENCE   1  (bases 1 to 1125)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1125)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:19:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,224
            CDSs (total)                      :: 3,198
            Genes (coding)                    :: 3,184
            CDSs (with protein)               :: 3,184
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1125
                     /organism="Aminobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_154587_length_1125_cov_2.781308"
                     /isolate="AMR_MDS_3026"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:3034609"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..989
                     /locus_tag="P5201_15255"
     CDS             <1..989
                     /locus_tag="P5201_15255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015556861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HRV99955.1"
                     /translation="SGEWSAEKDPPVKTLTDFTNYTEACRLLEEKFGGDVIEPPETFA
                     IYTQDDFLNDVFMSEDRYVTLKNLLLKKKNIILQGAPGVGKTYAAERLAYSIMEEKDT
                     NRVMTIQFHQSYSYEDFIMGYRPTENGFSMGKGPFYSFCKKAEPDDREHFFIIDEINR
                     GNLSKIFGELLMLIENDKRGKSIRLLYSDEQFQVPKNVHIIGMMNTADRSLAMIDYAL
                     RRRFAFFEFEPAFASTGFKRYQEFVANEKFDFLVQTVCSMNTAITDDPSLGSGFRVGH
                     SYFCAKDPADVDEIWLSDLVEYELIPLIKEYWFDDLSKVHEWSGKLRGALRV"
BASE COUNT          286 a          309 c          308 g          222 t
ORIGIN      
        1 attcgggtga atggagcgcc gaaaaggatc cacccgtcaa aacgctcacc gacttcacga
       61 actacaccga agcgtgccgg ctgttggaag agaagttcgg aggggatgta atagagccgc
      121 cggaaacctt cgcaatatac acacaagacg atttcctgaa cgatgtcttc atgagcgaag
      181 accggtacgt cacgctgaaa aacctcctgc tcaagaagaa gaacatcatc cttcagggag
      241 cgccgggcgt gggaaagacg tacgcggcgg agcgccttgc ctactcgatc atggaggaga
      301 aggacacgaa ccgggtcatg acgattcagt tccaccagag ctacagctac gaggatttca
      361 tcatggggta tcgcccgacg gagaacggtt tttcgatggg gaaggggccg ttctacagct
      421 tctgcaaaaa ggcggaaccg gacgaccgcg agcacttctt catcatcgac gagatcaacc
      481 gcgggaacct cagcaagata ttcggcgaac tcctgatgct catcgagaac gacaagcgcg
      541 gaaaatccat ccgcctcctc tattcggacg agcagtttca ggttcccaag aatgtacata
      601 tcatcggcat gatgaacacg gcggatcgca gcttggccat gatcgactat gcgctgcgcc
      661 ggcgtttcgc atttttcgaa ttcgagcccg ccttcgcatc gaccggattc aagaggtatc
      721 aggaatttgt agcgaatgaa aagttcgact ttctggtgca aaccgtctgc tccatgaaca
      781 ccgccatcac cgacgatccg tctctcggga gcggttttag ggtagggcac agctacttct
      841 gcgccaaaga ccccgccgac gtcgatgaaa tttggctctc cgatctggtg gaatacgaac
      901 tgattccgct catcaaagaa tactggttcg acgacctctc caaggtccac gaatggagcg
      961 ggaagctgcg cggtgcgctc cgtgtctgag aacatccgca tcaggaacgt ctactacatg
     1021 ctggcctacg cctaccacgc gctgaacgag gcggggtgca gaagcatcga gaccgaggat
     1081 ttcaagaacg ttcacgacct tctggccgcg attctgatta acggc
//
LOCUS       DAOTIL010001010         1125 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminobacteriaceae bacterium isolate AMR_MDS_3026
            NODE_154637_length_1125_cov_2.273832, whole genome shotgun
            sequence.
ACCESSION   DAOTIL010001010 DAOTIL010000000
VERSION     DAOTIL010001010.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868050
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminobacteriaceae bacterium (wastewater metagenome)
  ORGANISM  Aminobacteriaceae bacterium
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae.
REFERENCE   1  (bases 1 to 1125)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1125)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:19:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,224
            CDSs (total)                      :: 3,198
            Genes (coding)                    :: 3,184
            CDSs (with protein)               :: 3,184
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1125
                     /organism="Aminobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_154637_length_1125_cov_2.273832"
                     /isolate="AMR_MDS_3026"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:3034609"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..250)
                     /locus_tag="P5201_15260"
     CDS             complement(<1..250)
                     /locus_tag="P5201_15260"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRV99956.1"
                     /translation="MYDTTIRRTSARSFRKAACFAGLRWSREDRLDWGLDFLLRREDT
                     EEFRRYRERRLKELADAGVLFTNGVPLPVVKSTNWQPLK"
     gene            813..947
                     /locus_tag="P5201_15265"
     CDS             813..947
                     /locus_tag="P5201_15265"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRV99957.1"
                     /translation="MSGIWTGCSHNLSNASETVNQREWILGTEYIPAVAYLSDLVKIS
                     "
BASE COUNT          262 a          325 c          319 g          219 t
ORIGIN      
        1 gctttagtgg ttgccaattg gtacttttta ccaccggaag gggaacgccg ttcgtgaaca
       61 ggactccggc gtcggcgagc tccttgagcc tccgctcgcg gtagcggcgg aactcctccg
      121 tatcttcccg tctcagaaga aaatcgaggc cccagtcgag acggtcctct ctgctccagc
      181 gaagtccagc gaaacaggct gccttcctga acgacctcgc ggaggtgcgc cgaatcgtcg
      241 tgtcgtacat cgctgctcac atccttcgtc tatccccagc accgcgcgag acttcttcga
      301 aacaactctt cccggactct tcgatccggc tcttgcggcg ctcgtgaacg gttccccgat
      361 gattataaac gcacaacgcc cgttcaacga aagaacctcg gaggaaagag agcgggcaac
      421 tcttccacgc agtgcaaccc tgctttcgta cacagcgggt agatacttcg gaagggaaag
      481 tacgctccgt gctacgagat gctcgacggt caggtctgag gcgtccacgg aacggtgagc
      541 accatctctc tctgcaaaaa aggactgaac cgcatcaagg tagaggatac catcgatgga
      601 gactaagagg tgctctactt cgccccggag attggggaca tcgacaacat gctgacgagg
      661 acgatatact cggcaaggag gaatcccttc ttttcaagaa ggacggcatc tttcacgacg
      721 acgaggaaga ggaaaactaa acgtacgaag aggacgtcta cgtctgcgct atcgcgaata
      781 cctccgatac tgcgacgcaa aggagaacac cagtgagcgg gatatggacg gggtgctcgc
      841 acaatctgtc gaacgcgagt gaaaccgtca atcagcgcga gtggattctc gggacggagt
      901 acatccccgc ggtggcctat ctctcggatt tagtaaaaat ctcatgatag gaaaagacat
      961 atcgactacc ttcggggcgg cgtgtccgaa gaccttgttc ggacggcgcc gccccgctcg
     1021 cttttcacag aggggattcc gccggtcccc gcagttcgtt ttcgctcccc cggcgctctg
     1081 gaccgcgaag gggaagcagg cgttagaggc ggaacgcgac gacgt
//
LOCUS       DAOTIL010001011         1124 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminobacteriaceae bacterium isolate AMR_MDS_3026
            NODE_154776_length_1124_cov_3.680075, whole genome shotgun
            sequence.
ACCESSION   DAOTIL010001011 DAOTIL010000000
VERSION     DAOTIL010001011.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868050
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminobacteriaceae bacterium (wastewater metagenome)
  ORGANISM  Aminobacteriaceae bacterium
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae.
REFERENCE   1  (bases 1 to 1124)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1124)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:19:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,224
            CDSs (total)                      :: 3,198
            Genes (coding)                    :: 3,184
            CDSs (with protein)               :: 3,184
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1124
                     /organism="Aminobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_154776_length_1124_cov_3.680075"
                     /isolate="AMR_MDS_3026"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:3034609"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..395
                     /locus_tag="P5201_15270"
     CDS             <1..395
                     /locus_tag="P5201_15270"
                     /inference="COORDINATES: protein motif:HMM:NF014069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="HD domain-containing phosphohydrolase"
                     /protein_id="HRV99958.1"
                     /translation="ILCIPGPLRPVDRRLMQEHVTIGRDILVKEGFPERIALAVLHHH
                     ERMDGSGYPDGLKGEEIPLFARVVGIADVAEAMLSHRPYRPAHSREEVLRELEEHQGT
                     LYDRRTAEICISLLLEGFSVSEETGNKY"
     gene            complement(451..759)
                     /locus_tag="P5201_15275"
     CDS             complement(451..759)
                     /locus_tag="P5201_15275"
                     /inference="COORDINATES: protein motif:HMM:TIGR00377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043856 - anti-sigma factor antagonist
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="STAS domain-containing protein"
                     /protein_id="HRV99959.1"
                     /translation="MKIDVSRSGNGARISISGSMYVEDSASVREKLIALLDEGVYNLT
                     LDLSGLDYVDSSGLGVLISIHKRCLQKGGKMTITGLRGMVEELFKLTRLDLVFNVAAH
                     "
     gene            complement(848..>1124)
                     /locus_tag="P5201_15280"
     CDS             complement(848..>1124)
                     /locus_tag="P5201_15280"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRV99960.1"
                     /translation="DIRKIIATTDEEVAKSEKIISMIDTTASVMETTDDHVQNLAASM
                     EETAAAMENVATGSQEVSETAEDLKEMTGRFRVEKDEEGWTNVPAVV"
BASE COUNT          216 a          361 c          309 g          238 t
ORIGIN      
        1 agatcctctg tatccccggg ccgcttcgcc ccgtcgacag gcggctgatg caggagcacg
       61 taaccatcgg gagagatatc ctggtgaagg agggcttccc cgagcggata gcgctcgccg
      121 tgctccatca ccacgaacgg atggatggca gcgggtaccc ggacggcctg aagggtgagg
      181 aaatcccgct cttcgcgcgc gtcgtgggca tagcggacgt ggcggaagcg atgctgagcc
      241 atcgtccgta ccggccggca cactcgcggg aagaggtgct tcgcgagctg gaagagcatc
      301 agggaacgct ctacgacaga cgaacggcgg aaatctgcat ctctctgctt ctggaagggt
      361 tctccgtttc ggaggagacc ggcaacaaat actgaacggc aaaggggccc cctcgggagc
      421 cccttctttt tacgcgcagt ctttgagaac tcagtgcgca gccacgttga agacgaggtc
      481 gagccgagtg agtttgaaga gctcctcgac catgccgcga aggccggtga tcgtcatctt
      541 gccgcccttc tgaaggcacc gcttgtggat ggagatgagc acgccgaggc ctgagctgtc
      601 gacgtaatcc aaccccgaga gatccagcgt caggttgtac acgccttcgt cgagaagcgc
      661 tatcagcttc tcccgcaccg atgcggaatc ctccacgtac atacttccgg aaatgctgat
      721 ccgggctccg ttcccgctcc tgctgacatc gattttcatg gacgctcctc cttatatata
      781 tcgagacatc ttgttacact ccaacactgc ataaacatac gttcttccca cgaagatgca
      841 ggctccatta tacgacagcc ggcacgttcg tccacccctc ctcgtccttt tcgaccctga
      901 accgccccgt catttccttc aaatcctcgg cggtctcgct gacctcctgc gatcccgtgg
      961 ccacattctc catcgcagcc gcggtctcct ccatcgacgc cgcgaggttc tgcacgtggt
     1021 cgtccgtggt ctccatcacg ctcgccgtcg tgtcgatcat cgaaatgatc ttctccgact
     1081 tggcgacctc ctcgtccgtc gtggcgatga tcttccggat atcg
//
LOCUS       DAOTIL010001012         1123 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminobacteriaceae bacterium isolate AMR_MDS_3026
            NODE_155002_length_1123_cov_3.190075, whole genome shotgun
            sequence.
ACCESSION   DAOTIL010001012 DAOTIL010000000
VERSION     DAOTIL010001012.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868050
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminobacteriaceae bacterium (wastewater metagenome)
  ORGANISM  Aminobacteriaceae bacterium
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae.
REFERENCE   1  (bases 1 to 1123)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1123)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:19:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,224
            CDSs (total)                      :: 3,198
            Genes (coding)                    :: 3,184
            CDSs (with protein)               :: 3,184
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1123
                     /organism="Aminobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_155002_length_1123_cov_3.190075"
                     /isolate="AMR_MDS_3026"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:3034609"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            107..724
                     /locus_tag="P5201_15285"
     CDS             107..724
                     /locus_tag="P5201_15285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005660114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter small permease"
                     /protein_id="HRV99961.1"
                     /translation="MDGKSTKTPEERGLFLIARKVGDGLERAVEIPVFLLSIVMTVTV
                     LAGVFFRYIVRAPLGWSEELSRYLMIWMALLSVALCIWRHEHVGVTMFIKKLPRLLAK
                     IMVFVSNGLILYFLYVLARYGFRMAEGGKSQVSTALHTTMEWWLMAVPVSAVLCIIML
                     VCKMILDIRRKNLDEILMSEDIIDTVKREEGLDFGDVPLKEERSS"
     gene            721..>1123
                     /locus_tag="P5201_15290"
     CDS             721..>1123
                     /locus_tag="P5201_15290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014163859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter large permease subunit"
                     /protein_id="HRV99962.1"
                     /translation="MTGFISICFLILIVVGMPIGFVLGSTGLAAMLRMDMTSILQIVP
                     QRYFAGVDMFTLMAMPFFILAGEIMNKSGITGRLVKFANVLVGHLQGGLAHANILASV
                     FFAGITGAAVSDTAAIGSMLIPAMVEEGYDKD"
BASE COUNT          220 a          287 c          339 g          277 t
ORIGIN      
        1 ataaggatga aggcgtcgcg ctcgcacaga tgtacctcga cgccatcgaa caggcagtaa
       61 aggaggggaa atagggcgca agccctattc tccctccccg agttttatgg acgggaagag
      121 tacgaaaacg ccagaggaga gaggcctctt tctcattgcc cgtaaagtgg gcgacgggct
      181 ggagcgcgcg gtggagatcc cggtctttct cctttccata gtaatgaccg tcaccgtcct
      241 cgcgggggtc ttcttccggt atattgtccg ggcgccgctg ggttggtccg aggagctttc
      301 gcggtacttg atgatctgga tggcgctgct ctccgtagcc ctctgtatct ggcgccatga
      361 gcacgtcggc gtcacaatgt tcatcaagaa gcttccgcgt ctgctcgcga aaattatggt
      421 cttcgtatcg aacgggttga ttctctactt cctctatgtc ctggccagat acggcttccg
      481 gatggccgag ggaggaaaat ctcaggtttc aacggcgctg cacacgacga tggagtggtg
      541 gctcatggcc gtgccggtga gcgcggtgct ctgcatcatt atgctcgtct gcaagatgat
      601 cctcgatatc cgtcgaaaga atctcgatga gatcctgatg tcggaggata tcatcgatac
      661 agtaaagcgg gaggaaggcc tcgatttcgg ggatgttccc cttaaggagg aacgatcgtc
      721 atgaccggat tcatctcgat ctgttttttg attctcatcg tggtggggat gcctatcggt
      781 tttgtgctgg ggtccactgg tctggcggct atgctcagga tggatatgac ctccatcctt
      841 cagattgttc ctcagcgtta cttcgcgggg gtggacatgt tcacccttat ggcgatgccg
      901 tttttcattc tggcaggaga gatcatgaac aagagcggta tcacgggccg ccttgtcaag
      961 ttcgcaaatg tgcttgtcgg ccatcttcaa ggaggactgg cgcacgccaa catcctggct
     1021 tccgtctttt tcgccgggat cacaggggcg gcggtgagcg ataccgccgc catcggctcg
     1081 atgctcatcc cggcgatggt agaggaaggg tacgataagg act
//
LOCUS       DAOTIL010001013         1123 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminobacteriaceae bacterium isolate AMR_MDS_3026
            NODE_155044_length_1123_cov_2.692884, whole genome shotgun
            sequence.
ACCESSION   DAOTIL010001013 DAOTIL010000000
VERSION     DAOTIL010001013.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868050
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminobacteriaceae bacterium (wastewater metagenome)
  ORGANISM  Aminobacteriaceae bacterium
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae.
REFERENCE   1  (bases 1 to 1123)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1123)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:19:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,224
            CDSs (total)                      :: 3,198
            Genes (coding)                    :: 3,184
            CDSs (with protein)               :: 3,184
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1123
                     /organism="Aminobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_155044_length_1123_cov_2.692884"
                     /isolate="AMR_MDS_3026"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:3034609"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>1123
                     /locus_tag="P5201_15295"
     CDS             <1..>1123
                     /locus_tag="P5201_15295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008522679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="4Fe-4S double cluster binding domain-containing
                     protein"
                     /protein_id="HRV99963.1"
                     /translation="LKKIEIPAEWAEATADPVLVSGLPEEIPDFVREVCMPINAQKGD
                     SLPVSAFMGREDGQFPLGTAAFEKRGVAVNVPEWKSDKCIQCNQCSMVCPHAAIRPFL
                     LNDEEAAAAPEGFGAVDVKGKEFPGHKYKMQVNALDCLGCGNCADICPVGALEMKPLA
                     SQEKEIPNWNFALTVSDKSEETNKFTVKGSQFSQPLLEFSGACAGCGETPYIKLVTQL
                     FGDRMIVANATGCSSIWGGSAPSMPYTVNAKGQGPAWANSLFEDNAEYGYGMKLSLNA
                     TMTYIVDAMKGLLEMDIPEDMKAAFSEWIESMDDGEKSKAAAEKVLAVLGRDIGAEGN
                     KLLCEIACRKDYLVKKSVWIFGGDGWAYDIGFGGLDHVLA"
BASE COUNT          256 a          318 c          334 g          215 t
ORIGIN      
        1 cctgaaaaag atcgagatac ccgccgaatg ggctgaagca acggccgatc cggttcttgt
       61 gagcggtctt cccgaggaga ttcctgattt tgtacgggaa gtctgcatgc ccataaacgc
      121 ccagaagggc gacagccttc ctgtaagtgc ctttatgggg cgcgaagatg gtcagttccc
      181 cttgggaacg gcggctttcg agaaacgggg cgtcgccgtc aatgttcccg agtggaagag
      241 cgacaagtgc atccagtgca atcagtgctc catggtgtgc ccccatgccg cgatccgtcc
      301 cttcctgctg aacgacgaag aggctgcagc cgcgccggaa ggcttcggcg cagttgacgt
      361 aaagggcaag gagttcccgg gccataagta caagatgcag gtcaacgctc tcgactgcct
      421 cggatgcgga aactgcgccg acatctgccc ggtgggcgcc ctcgagatga agcctctcgc
      481 gagccaggag aaggaaatcc cgaactggaa ctttgccctc accgtttccg acaagtccga
      541 ggagacgaac aagtttaccg ttaagggaag ccagttctcc cagcccctcc ttgagttctc
      601 cggagcctgc gcgggatgcg gagaaactcc ctacatcaag ctcgtcacgc agcttttcgg
      661 cgacaggatg atcgtggcca acgccacagg gtgctcttcc atctggggag gttccgcgcc
      721 gagcatgccc tacacagtca acgcaaaggg acagggacct gcatgggcca actccctctt
      781 cgaagacaac gccgaatacg gctacggcat gaaactctcc ctcaacgcca ccatgacgta
      841 catcgtcgat gcgatgaagg gccttctgga gatggatatt cccgaagaca tgaaggccgc
      901 tttcagcgag tggattgaat ccatggacga cggagaaaaa tccaaggctg cggcggaaaa
      961 ggttcttgcg gttctcggcc gtgatatcgg ggcggaaggc aataagctcc tgtgcgagat
     1021 agcctgcaga aaggactatc tcgtcaagaa gtccgtatgg atcttcggag gtgacggctg
     1081 ggcatacgat atcggtttcg gcggcctcga ccatgtcctt gcc
//
LOCUS       DAOTIL010001014         1123 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminobacteriaceae bacterium isolate AMR_MDS_3026
            NODE_155066_length_1123_cov_2.461610, whole genome shotgun
            sequence.
ACCESSION   DAOTIL010001014 DAOTIL010000000
VERSION     DAOTIL010001014.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868050
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminobacteriaceae bacterium (wastewater metagenome)
  ORGANISM  Aminobacteriaceae bacterium
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae.
REFERENCE   1  (bases 1 to 1123)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1123)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:19:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,224
            CDSs (total)                      :: 3,198
            Genes (coding)                    :: 3,184
            CDSs (with protein)               :: 3,184
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1123
                     /organism="Aminobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_155066_length_1123_cov_2.461610"
                     /isolate="AMR_MDS_3026"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:3034609"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..341)
                     /locus_tag="P5201_15300"
     CDS             complement(<1..341)
                     /locus_tag="P5201_15300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014807105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016624 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, disulfide as
                     acceptor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent enzyme"
                     /protein_id="HRV99964.1"
                     /translation="MEISKETLLGMYRKMLTIRRFEERAAELYAAGRMPGFVHLYVGE
                     EAVATGVCASLSDKDFITSTHRGHGHVIAKGGKTDLMMAELYGKVTGYCKGKGGSMHI
                     ADVDLGILGANG"
     gene            complement(639..>1123)
                     /locus_tag="P5201_15305"
     CDS             complement(639..>1123)
                     /locus_tag="P5201_15305"
                     /inference="COORDINATES: protein motif:HMM:NF012968.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit
                     alpha/FixB family protein"
                     /protein_id="HRV99965.1"
                     /translation="PFVERMRVEGIAEGRNVEILEVVKKMSAKKDIQEARVLVAGGRG
                     MGGPENFAVLEELAEVLGGTVASSRASVDAGWVGKDRQVGQTGKTVRPDLYIACGISG
                     AIQHLAGMEESGIIVAINKDPSAPIFEAADYGVVGDALKIVPMLAESIRNARARKAEV
                     "
BASE COUNT          211 a          396 c          293 g          223 t
ORIGIN      
        1 ccgttggccc cgaggatgcc gaggtcgacg tccgcgatgt gcatcgagcc gcccttcccc
       61 ttgcagtacc ccgtcacctt gccgtacagc tccgccatca tcagatcggt cttcccgccc
      121 ttggcgatca catgcccgtg ccccctgtgg gtgctcgtga tgaaatcctt gtcgctcaaa
      181 gaggcgcaca cgccagtcgc cacagcctcc tcacctacgt aaaggtggac aaaccccggc
      241 atcctccccg ccgcgtacag ctccgcggcc ctctcctcga acctcctgat ggtcagcatc
      301 ttccggtaca tcccgagcaa cgtttcctta ctgatctcca tacccgtttt ccctccttgc
      361 acaaaaaaaa cttaatttcc aggcttcact gtaaacacag ccttatcgga attatcatat
      421 acgctaagac ctgtcaagga ttttcacgga aagagaagaa acgtatactc cgtacaaaac
      481 ttttcttttc catacgcaga gcggccctct tcacgagagt gaaaagggcc gccggggatg
      541 aagcataatt acgctcttcg caagaaagag gcgggaagcg tccgtggagg agcgtccgga
      601 ggtccgaagc gcccgcgcaa cggagagact gccgcgcgct acacctccgc cttccgggcg
      661 cgcgcgttcc ggatgctctc ggcgagcatc gggacgatct tcagcgcatc gccgaccacg
      721 ccgtagtcgg ccgcctcgaa gatgggagcc gaggggtcct tgttgatggc gacgatgatc
      781 ccggactcct ccattccggc gaggtgctgg atggcgccgg agatgccgca ggcgatgtag
      841 aggtccggac ggacagtctt tcccgtctgc cccacctgcc ggtccttccc gacccagccc
      901 gcgtcgacgc tcgcccgcga ggaggcgacc gttccgccga ggacctccgc cagctcttcg
      961 agaacggcga agttctcggg tccgcccatt ccgcgccctc cggcgacgag cacccgggcc
     1021 tcctgtatgt cctttttcgc gctcatcttc ttcacgactt cgagaatctc cacattccgc
     1081 ccctccgcga tcccttcgac gcgcatccgt tccacaaagg gct
//
LOCUS       DAOTIL010001015         1121 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminobacteriaceae bacterium isolate AMR_MDS_3026
            NODE_155440_length_1121_cov_3.381801, whole genome shotgun
            sequence.
ACCESSION   DAOTIL010001015 DAOTIL010000000
VERSION     DAOTIL010001015.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868050
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminobacteriaceae bacterium (wastewater metagenome)
  ORGANISM  Aminobacteriaceae bacterium
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae.
REFERENCE   1  (bases 1 to 1121)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1121)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:19:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,224
            CDSs (total)                      :: 3,198
            Genes (coding)                    :: 3,184
            CDSs (with protein)               :: 3,184
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1121
                     /organism="Aminobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_155440_length_1121_cov_3.381801"
                     /isolate="AMR_MDS_3026"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:3034609"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1121)
                     /locus_tag="P5201_15310"
     CDS             complement(<1..>1121)
                     /locus_tag="P5201_15310"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRV99966.1"
                     /translation="AFVPFVPDEGVYYMHSLLQDYLRDRFEQTGETFRRETYRRAGRA
                     CETVGEYFHATQFHIETGNYEAILSMPFTTQYFYNFRERSVIDLFERLIDECPEETLL
                     KHPLALVTFGQQFFRDRRMLPFSKTTALVQKLLDAPSGLSEKELLRIRGEFSILMSFP
                     QFNDIAKMSEYHRRAYDSLRHLSDPPRSVIFGGAMPWTMSGASVLFLYWRESGALKKT
                     LSVMDECLPYYVALAGGHGAGGEYVLRAEAALFRGDDVEAEVMCHTALFEARSAGQIG
                     NCLCASLVLARIMLLRGDGDSYTSIRRSILKDAREAQQTSLLRIGDLCLAVTDLALGR
                     LDDLPPWLRDAESIRKVVYTDGQPYAMMLHGMALLQEER"
BASE COUNT          220 a          348 c          340 g          213 t
ORIGIN      
        1 gcgttcttcc tgaagcagcg ccattccgtg aagcatcatc gcatacggct gtccgtcggt
       61 gtagacgact ttccggatgc tctcggcgtc gcggagccag ggaggcagat cgtcgaggcg
      121 tccgagcgcc aggtcggtaa cggccaggca gaggtccccg atgcgcagga gcgacgtctg
      181 ctgcgcttcc cgggcgtcct tcagaatgct gcgacgtatg gaggtgtacg aatcgccgtc
      241 gccgcgcagc agcatgatac gggccagcac cagcgatgcg cagagacagt tgccgatctg
      301 ccccgcgctg cgcgcttcaa aaagcgcggt atggcacatg acctccgctt cgacgtcgtc
      361 tccgcggaag agcgcggctt cggcgcgcag gacatactct ccgccggcgc cgtgccctcc
      421 cgcgagggcg acgtaatacg gcaggcattc gtccatcacc gagagcgttt ttttcagggc
      481 gccgctctct ctccagtaga ggaagagcac cgacgctccg ctcatcgtcc acggcatggc
      541 ccccccgaaa ataaccgacc gcggagggtc cgacagatga cgcagggaat cgtacgcccg
      601 cctgtgatat tcgctcatct tggcgatatc gttgaactgg ggaaaggaca tcaggatgga
      661 aaactccccc ctgatccgca gcagctcctt ctcggagagg ccggaaggag cgtcgaggag
      721 tttctgcacc agcgccgtcg tttttgaaaa tgggagcatt cttctgtccc tgaaaaactg
      781 ctggccgaac gtcacaagag ccagcgggtg tttcaacagc gtttcctccg ggcactcgtc
      841 gataaggcgc tcgaagagat cgatgacgct tcgctccctg aaattgtaaa aatactgcgt
      901 tgtgaacggc atcgagagga tcgcctcgta gttcccggtc tcgatgtgga actgcgtcgc
      961 gtgaaagtac tcgccgaccg tctcgcaggc gcgccccgcc ctgcggtacg tctcccgccg
     1021 gaacgtctcg cccgtttgtt cgaacctgtc cctcagatag tcctgaagga ggctgtgcat
     1081 atagtacacg ccctcgtccg gaacgaaggg aacgaaggcg t
//
LOCUS       DAOTIL010001016         1121 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminobacteriaceae bacterium isolate AMR_MDS_3026
            NODE_155462_length_1121_cov_2.931520, whole genome shotgun
            sequence.
ACCESSION   DAOTIL010001016 DAOTIL010000000
VERSION     DAOTIL010001016.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868050
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminobacteriaceae bacterium (wastewater metagenome)
  ORGANISM  Aminobacteriaceae bacterium
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae.
REFERENCE   1  (bases 1 to 1121)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1121)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:19:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,224
            CDSs (total)                      :: 3,198
            Genes (coding)                    :: 3,184
            CDSs (with protein)               :: 3,184
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1121
                     /organism="Aminobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_155462_length_1121_cov_2.931520"
                     /isolate="AMR_MDS_3026"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:3034609"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..728
                     /locus_tag="P5201_15315"
     CDS             <1..728
                     /locus_tag="P5201_15315"
                     /inference="COORDINATES: protein motif:HMM:NF018513.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0031317 - tripartite ATP-independent
                     periplasmic transporter complex [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="TRAP transporter large permease"
                     /protein_id="HRV99967.1"
                     /translation="VVSWFISKKRNYPKGVKVPRRLLLKEFFGAFWALMLTVLILYGI
                     VGGFFSPTEAAMVACVYAVVIGMFVYKDLSFKELPAVILETAITTASIIVLVGLANLF
                     AWILTSEQIPQMVADLLLSITSNKYLIILLINFLLLFVGTFMETIAALLTLFPTLLAV
                     ALRVGIDPIHFAMIAVLNLMIGLTTPPVGVCLFVASSIGKVSISRIARAGLPFLAVSI
                     FVLLLVSYIPALSTWLPNLLFNR"
     gene            789..>1121
                     /locus_tag="P5201_15320"
     CDS             789..>1121
                     /locus_tag="P5201_15320"
                     /inference="COORDINATES: protein motif:HMM:NF012334.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HRV99968.1"
                     /translation="MTSKFSLEGKRALVTGGGSGIGLAISQGIVENGGSVVIVGRREE
                     LLKEACDSMGPKASYMVHDVTDLPGNVGFVKKLEAGGPVDILVNNAGQNIKKPVLETT
                     DEIYHNILE"
BASE COUNT          226 a          316 c          317 g          262 t
ORIGIN      
        1 tggtggtctc ctggtttata tcgaagaaac ggaactaccc caagggagtg aaagttcccc
       61 gccgactgct gctgaaggag ttcttcggcg ccttctgggc gttgatgctc acagtgctca
      121 tcctctatgg catcgtcgga ggttttttca gtcccaccga ggctgcgatg gttgcctgtg
      181 tctacgccgt tgtgatcggc atgttcgtct acaaggatct ctcgttcaaa gagctcccgg
      241 cggtgattct cgaaaccgca atcacgacgg cgtcgatcat cgtgcttgtc ggcctggcga
      301 acctttttgc ctggattctc accagcgagc aaatccccca gatggtggcc gatttattgc
      361 tgagtatcac gagcaacaaa tatttaatta tactgttgat caacttcctg ctcctttttg
      421 tagggacgtt catggagacg atcgccgcgc tgctcacgct cttcccgacg ctcctcgccg
      481 tagcgctgcg tgtggggata gacccgattc attttgctat gatcgccgtg ctcaatctga
      541 tgatcggatt gaccaccccc cctgtgggcg tctgtctctt cgtcgcttcc agcatcggga
      601 aggtctccat cagccgtatc gcgcgggcag ggctgccatt tcttgctgtg agcatcttcg
      661 tcctgctgct ggtctcctac atcccagcgc tttcgacgtg gctgcccaat ctgctcttca
      721 accgctgaag cactaccgcc gggagaataa gcggcgtatg tacgagaaag tgaaaggaga
      781 aatacgacat gacatccaaa ttttcgcttg agggaaaacg ggccctggta acgggcggcg
      841 gaagcggcat cggtctcgcc atctcgcagg ggatcgtgga gaacggcggt tccgtggtca
      901 tcgtaggccg gagggaggaa ctcctcaagg aagcttgcga ctccatgggg ccgaaagcgt
      961 cgtacatggt ccacgacgtc acggatctgc cgggcaacgt cgggttcgtc aagaagctgg
     1021 aagcgggcgg gcctgtcgac atcctcgtga acaacgccgg acagaacatc aagaagcccg
     1081 tcttggagac cacggacgag atctaccata acatcctcga g
//
LOCUS       DAOTIL010001017         1117 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminobacteriaceae bacterium isolate AMR_MDS_3026
            NODE_156290_length_1117_cov_3.752354, whole genome shotgun
            sequence.
ACCESSION   DAOTIL010001017 DAOTIL010000000
VERSION     DAOTIL010001017.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868050
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminobacteriaceae bacterium (wastewater metagenome)
  ORGANISM  Aminobacteriaceae bacterium
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae.
REFERENCE   1  (bases 1 to 1117)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1117)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:19:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,224
            CDSs (total)                      :: 3,198
            Genes (coding)                    :: 3,184
            CDSs (with protein)               :: 3,184
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1117
                     /organism="Aminobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_156290_length_1117_cov_3.752354"
                     /isolate="AMR_MDS_3026"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:3034609"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..439
                     /locus_tag="P5201_15325"
     CDS             <1..439
                     /locus_tag="P5201_15325"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRV99969.1"
                     /translation="NDSPLDLKCVGDINIRALNAFIGALQGFLTIDGNPLTDPQFLQR
                     FLTGLVGGMAIRDFRETSFNLKGTWDSPEMLDLKVSRGSAPLTIPRSNGSTGKNETQI
                     KITVEIPTGSGSDTSASTEEQVKKQLLENIMKSIIKPGSTEND"
     gene            576..>1117
                     /locus_tag="P5201_15330"
     CDS             576..>1117
                     /locus_tag="P5201_15330"
                     /EC_number="3.6.1.54"
                     /inference="COORDINATES: protein motif:HMM:NF012376.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008758 - UDP-2,3-diacylglucosamine
                     hydrolase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-2,3-diacylglucosamine diphosphatase"
                     /protein_id="HRV99970.1"
                     /translation="MSSLKYRSVFLSDFHLGTRWCRAKSLVSFLGSMECEKLYLIGDI
                     IDGWKLKRSPGWPNSHNSVIRKILKMSKKTPVLYIPGNHDDFMKEFNGYSFGGVKICE
                     NAIHTSPEGKRFLVVHGHEFDVAVMYKPWLAFLGDRAYDISLHLNTGLNAIRSFLGFG
                     YWSLSEFLKRKVKDAVKFIG"
BASE COUNT          270 a          309 c          316 g          222 t
ORIGIN      
        1 gaacgactcc cccctcgact tgaaatgcgt cggcgacatc aatatcaggg cgctcaacgc
       61 ttttatcggc gctcttcagg ggtttctgac catcgacggc aatccgctga cggaccccca
      121 gttcttgcag cgcttcctga cggggctcgt cggcggaatg gcgatccgcg acttccggga
      181 gacctcgttc aacctcaagg ggacgtggga ctctccggag atgctcgacc tgaaggtatc
      241 ccgggggagc gctccgctga ccattccgag gtccaacggc tccaccggca agaacgaaac
      301 gcagataaag attactgtgg agatccccac ggggtcgggg tcggacacct cggcgagcac
      361 ggaggagcaa gtaaaaaaac agcttctgga gaacatcatg aagtccatca tcaaacccgg
      421 ctcgacggaa aacgactgag cggctgcaaa agaggtatac ttatacggtt ggagtcgacg
      481 tacggagata caaccgatac attttcgtat cattcgcacg gaacttccgt taccgaaagg
      541 agttatgcta tgacgcaaga aaagagaaga aagggatgag ttctctgaaa taccggtccg
      601 tcttcctctc cgatttccac ctcggaacgc gctggtgcag agcgaagagt ctggtgtcat
      661 tcctcgggtc gatggagtgc gagaagctct acctgatcgg cgacatcatc gacggatgga
      721 agctgaagag aagccccgga tggccgaact cgcacaactc ggtaatccgg aagatactga
      781 agatgtcgaa gaaaacgccg gtgctctaca tccccggcaa ccacgacgac tttatgaagg
      841 agttcaacgg ctactcgttc ggcggcgtga agatttgcga gaacgcgatc cacacttccc
      901 ccgaagggaa acgattcctg gtggtccacg gacatgagtt cgacgtcgcg gtgatgtaca
      961 agccctggct cgccttcctc ggagaccgtg cctacgatat ctctctgcat ctgaacacgg
     1021 ggttgaacgc cattcgttcc ttcctcggct tcgggtactg gtcgctctcc gagtttctga
     1081 agcgaaaggt caaggacgcc gtgaagttca tcggcaa
//
LOCUS       DAOTIL010001018         1117 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminobacteriaceae bacterium isolate AMR_MDS_3026
            NODE_156331_length_1117_cov_2.862524, whole genome shotgun
            sequence.
ACCESSION   DAOTIL010001018 DAOTIL010000000
VERSION     DAOTIL010001018.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868050
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminobacteriaceae bacterium (wastewater metagenome)
  ORGANISM  Aminobacteriaceae bacterium
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae.
REFERENCE   1  (bases 1 to 1117)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1117)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:19:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,224
            CDSs (total)                      :: 3,198
            Genes (coding)                    :: 3,184
            CDSs (with protein)               :: 3,184
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1117
                     /organism="Aminobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_156331_length_1117_cov_2.862524"
                     /isolate="AMR_MDS_3026"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:3034609"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1117)
                     /locus_tag="P5201_15335"
     CDS             complement(<1..>1117)
                     /locus_tag="P5201_15335"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HRV99971.1"
                     /translation="FFSSLLAAVVALRTGRRLKQETARADELAVHARAASVAKSEFLA
                     NMSHEIRTPMNGVVGMTGLLLDTDLTEEQRRYAEIVRVSAESLLSLINDILDFSKIEA
                     GKLDLEILNFDLSSLMDDFILTLAVSAEEKGIELLCGVDPDVPVLLRGDPGRLRQILT
                     NFVSNAVKFTHEGEVETRVSLVEESDRDALLRFSVRDTGIGIAEEKMGLLFTKFSQAD
                     ASTTRKYGGTGLGLAISKQLAEMMGGSVGAASAEGKGSEFWCTARLEKQPENLRRELR
                     PATDLRNVRVLIVDDNATNRRLLTVRLTSWGMRPESVSGGSAALASLDAALREGDPFR
                     LAVVDMQMPEMDGETFGSLVRADGRFGGVRGRERLAAS"
BASE COUNT          192 a          387 c          335 g          203 t
ORIGIN      
        1 agaagccgcc aggcgctctc gcccgcgtac gccgccgaac cgtccgtccg cacgcacgag
       61 acttccgaac gtctcgccgt ccatctccgg catctgcata tccaccacgg caaggcggaa
      121 cggatcgccc tcccgcagcg cggcgtcgag cgacgcgagc gcggcgcttc cgccggagac
      181 gctctcgggg cgcatccccc acgacgtcag ccgaacggtc aaaagccgtc tgttggtcgc
      241 gttgtcgtcc acgatcagga cgcgcacgtt cctcaggtcg gttgcgggac ggagttcgcg
      301 ccgcaggttc tccggctgtt tttccaggcg ggcggtacac cagaattccg agcccttccc
      361 ctctgcgctc gccgcgccga ccgagccgcc catcatctcc gcaagctgct tcgagatggc
      421 aagccccagc cccgtgccgc cgtacttgcg cgtcgtcgag gcgtccgcct gactgaactt
      481 cgtgaagagc aatcccatct tctcctcggc gatccctatc cccgtatcgc gcaccgagaa
      541 gcggagcaac gcgtcgcggt cgctctcttc caccagagag actctcgtct ccacttcccc
      601 ttcgtgcgtg aacttcaccg cgttgctcac gaagttggtg aggatctgcc gcagacgccc
      661 cggatcgccg cgaagcagca cgggaacgtc cggatcgacg ccgcacagca gctcgatccc
      721 cttctcctcg gcgctcaccg ccagcgtcag gataaagtcg tccatgaggc tggacagatc
      781 gaagttgagg atctcgaggt cgagctttcc ggcctctatc ttggagaagt ccagaatatc
      841 gttgatgaga gagaggagag attccgcgct gaccctgacg atctcggcat accgccgctg
      901 ctcctcggtc aggtccgtat cgagcagcaa accggtcatc ccgacgacgc cgttcatggg
      961 cgtgcggatc tcgtggctca tgtttgcgag gaattcgctc ttggcaacgc tggcggctct
     1021 tgcgtggacg gccagttcgt cggcgcgggc agtctcctgc ttgaggcggc ggcctgttcg
     1081 caaggcgacg acggcggcaa gaagcgatga gaagaac
//
LOCUS       DAOTIL010001019         1116 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminobacteriaceae bacterium isolate AMR_MDS_3026
            NODE_156642_length_1116_cov_2.043355, whole genome shotgun
            sequence.
ACCESSION   DAOTIL010001019 DAOTIL010000000
VERSION     DAOTIL010001019.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868050
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminobacteriaceae bacterium (wastewater metagenome)
  ORGANISM  Aminobacteriaceae bacterium
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae.
REFERENCE   1  (bases 1 to 1116)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1116)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:19:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,224
            CDSs (total)                      :: 3,198
            Genes (coding)                    :: 3,184
            CDSs (with protein)               :: 3,184
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1116
                     /organism="Aminobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_156642_length_1116_cov_2.043355"
                     /isolate="AMR_MDS_3026"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:3034609"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>1116
                     /locus_tag="P5201_15340"
     CDS             <1..>1116
                     /locus_tag="P5201_15340"
                     /inference="COORDINATES: protein motif:HMM:NF036799.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004826 - phenylalanine-tRNA ligase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase beta subunit-related
                     protein"
                     /protein_id="HRV99972.1"
                     /translation="PSPEITVRSAADGEETKTLDGKNHSLDPGDMLITSGGLAVGIAG
                     VMGGENSEILESTRTVFLESANFDAIRVSRTSRRLGISSEAAFRYARIVDTRIAQTAL
                     KYISSLLEEWGAAEGGYAILSASTKLPEEKSVTLTKGKLKKILLTEDMKEASAILSRL
                     GLVEISSTSETATYSVPSWRPDISIEEDLIEEVGRIRGYNETLESRLPQALYGRGDIG
                     RTTRLKGEIRSTLLSRGYVELVNYSFLSPSFVELLRLPAADRRARPLLLANPMSVEQS
                     RMRTTLLPGMLRSVEQSVASGWKTAVRVFELGRVFFPLDEGGHEEIERICGVAYGGKD
                     SRLPYGPADADDLFSLKADILALAESRGVVLRFVQGEE"
BASE COUNT          222 a          339 c          340 g          215 t
ORIGIN      
        1 ccgtcgccgg agattaccgt tcgctccgcc gccgacggcg aagaaaccaa aacgctcgac
       61 ggaaagaatc acagtcttga cccaggcgat atgctcatca caagcggcgg tcttgccgtt
      121 ggcatcgccg gcgtgatggg aggagagaac agcgagatcc ttgagtccac gagaaccgtt
      181 ttccttgagt cggccaattt cgacgccatc agggtaagca gaacctcccg cagactcggc
      241 atctcctccg aggccgcctt ccggtatgcg cgtatcgtcg atacgcgcat cgcgcaaacg
      301 gccctgaagt atatctcctc gcttctggag gagtggggcg ccgcggaagg agggtacgcc
      361 atcctctccg cgtcgacgaa gcttcccgag gagaagtctg tgacgctgac caagggaaaa
      421 ttgaagaaga tccttctcac ggaggatatg aaagaggcgt ccgcgatact ctcccgcctc
      481 ggcctcgtcg aaatttcctc gacgtccgaa acggcgacat attccgttcc ctcgtggcgt
      541 cccgacatct ccatcgagga ggacctcatc gaagaggtcg gcaggatccg cgggtacaac
      601 gaaacgctgg agtcccgtct tccgcaggcg ctctacgggc gaggagacat cggccgcacg
      661 accagactca agggagagat acgatcgacg ctgctctcca ggggatacgt ggagctcgtg
      721 aactacagct ttctctcccc ttcgttcgtc gagttacttc gtctcccggc cgcggatcgc
      781 agggcccgcc cgctgttgct ggccaacccg atgagcgtgg agcagtcgag gatgcgtacg
      841 acgcttcttc ccggaatgct tcgaagcgtc gagcagagcg ttgcttcagg gtggaagacc
      901 gcggtgcgcg tctttgagct cggcagggtc ttctttcctc ttgatgaagg cgggcacgag
      961 gagattgaac gtatatgcgg cgtcgcctac ggcggaaagg actcccggct tccgtacgga
     1021 ccggccgacg cggacgatct cttttcgctc aaagcggata tcctcgccct cgccgagagc
     1081 cgcggcgttg ttctccgttt cgtccaggga gaggag
//
LOCUS       DAOTIL010001020         1115 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminobacteriaceae bacterium isolate AMR_MDS_3026
            NODE_156818_length_1115_cov_2.352830, whole genome shotgun
            sequence.
ACCESSION   DAOTIL010001020 DAOTIL010000000
VERSION     DAOTIL010001020.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868050
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminobacteriaceae bacterium (wastewater metagenome)
  ORGANISM  Aminobacteriaceae bacterium
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae.
REFERENCE   1  (bases 1 to 1115)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1115)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:19:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,224
            CDSs (total)                      :: 3,198
            Genes (coding)                    :: 3,184
            CDSs (with protein)               :: 3,184
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1115
                     /organism="Aminobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_156818_length_1115_cov_2.352830"
                     /isolate="AMR_MDS_3026"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:3034609"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            207..1040
                     /locus_tag="P5201_15345"
     CDS             207..1040
                     /locus_tag="P5201_15345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015555995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HRV99973.1"
                     /translation="MRHLKSLVLSLLVPALFLCLWAYMAKRTANDVVLPPIGQVATHF
                     ASPGTSLIGMGSLVSNILISLLRVLAGYLIAVLLAIPAGLLLGHNRLLSELFTPFLGL
                     FRPIPPLAWVPLVLAWFGIASFATLFGVEEGEWYVFLNNLKFSMVFIIFIGAFYPILS
                     GTMHGVSGVRSILVDSARVLGASDRDIFLKVLLPASLPSIVNGLRIGLGVAWMCLVSA
                     EMLPGSISGVGYLITHAYTVARTDIVIVGMIAIGLLGAAMDRGFLFVEERAFQWQRLT
                     R"
BASE COUNT          170 a          380 c          329 g          236 t
ORIGIN      
        1 agtctacctc gacgtgctga acggcatgaa cagcttcaag ggctctctga agggcaagac
       61 gctcgaagag gcgattccgt tggtcttcga cttctccttc aacaagtaaa cggaagacga
      121 ttcacggcct ccgcgcacgg gggtccctcg tccatcggcg gaggggcccc cgacgcgcgc
      181 gggggcggaa aggatatgcg accgatatgc ggcatctgaa atctcttgtt ctctcgcttc
      241 ttgttccagc gctcttcctg tgcctgtggg cctacatggc gaagcgcacg gcaaacgacg
      301 tcgtcctccc ccccataggg caggtggcga cgcacttcgc gtctccgggg acgagcctga
      361 tcggaatggg ctctctcgtc tccaacattc tcatcagcct gctccgggtg ctcgcaggct
      421 acttgatcgc cgttctcctc gcgatccccg cggggctgct gctggggcac aatcgccttc
      481 tctccgaact cttcaccccc tttctcggac tcttccgccc gataccgccc ctcgcctggg
      541 ttccgctcgt gctcgcgtgg ttcggcatcg ccagcttcgc cacgctcttc ggcgtggagg
      601 agggggagtg gtacgtcttc ctgaacaacc tgaagttctc gatggtcttc atcatcttca
      661 tcggcgcatt ctaccccatc ctctccggga cgatgcacgg cgtctccggc gtgcgctcca
      721 tcctcgtcga ctccgcgcgg gtgctgggag cctccgaccg cgacatcttc ctcaaggtgc
      781 tgctgccggc gtctcttccc tccatcgtga acgggcttcg catcgggctg ggcgtggcgt
      841 ggatgtgcct cgtctcggct gagatgctcc ccggaagcat ctccggcgtg ggctacctga
      901 tcacgcacgc ctacaccgtc gcccgcaccg acatcgttat cgtcggcatg atcgccatcg
      961 gactgctcgg cgccgcgatg gaccggggat tcctcttcgt cgaggagcgc gccttccaat
     1021 ggcagcggct cacgaggtga cggcgatgca ggagaacatc gtgatccggg cggcgcatat
     1081 cggaaagacc ttcgcctccc gctcgggaaa ggagc
//
LOCUS       DAOTIL010001021         1115 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminobacteriaceae bacterium isolate AMR_MDS_3026
            NODE_156883_length_1115_cov_1.715094, whole genome shotgun
            sequence.
ACCESSION   DAOTIL010001021 DAOTIL010000000
VERSION     DAOTIL010001021.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868050
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminobacteriaceae bacterium (wastewater metagenome)
  ORGANISM  Aminobacteriaceae bacterium
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae.
REFERENCE   1  (bases 1 to 1115)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1115)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:19:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,224
            CDSs (total)                      :: 3,198
            Genes (coding)                    :: 3,184
            CDSs (with protein)               :: 3,184
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1115
                     /organism="Aminobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_156883_length_1115_cov_1.715094"
                     /isolate="AMR_MDS_3026"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:3034609"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            67..375
                     /locus_tag="P5201_15350"
     CDS             67..375
                     /locus_tag="P5201_15350"
                     /inference="COORDINATES: protein motif:HMM:NF013540.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HRV99974.1"
                     /translation="MSGNNAFIGSSLDDFLHEEGILPECEAEANKRIVAWQLEQYMQE
                     HNLKKTEFAERLGTSRSQLNRLLDAKDTSINLNTMAKIARAMGKTFRIQFEQRSEERP
                     "
     gene            complement(450..>1115)
                     /locus_tag="P5201_15355"
     CDS             complement(450..>1115)
                     /locus_tag="P5201_15355"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRV99975.1"
                     /translation="NGRTASRSAYLEFAGSREGNEFLLVDRPELFSFMLYSLPEDIKL
                     ESSHFTEMTRFVFTLLAEGGDDVFSTEEKKIPLGFLLEREVKKIGGPGTEFTVGNSYL
                     LDANTSLLTEKPGTTASGVMEMKRIDKGAFFKVEERLTQGSVWLRISTAQGNGWELES
                     ILLKKSWATSSEMRAVFPSLIVTDERKKTTVECLKYTLPVSFVVPREVLPLVKSIRLD
                     RLR"
BASE COUNT          324 a          273 c          279 g          239 t
ORIGIN      
        1 aaacaccgga gagagagctg aaaacagcaa gagagcgggt caaacagata cttgggaggt
       61 gaagagatga gtggcaataa tgcgttcatt ggaagtagcc tggacgactt cctgcacgaa
      121 gagggaattc tgccggagtg cgaagcggag gcgaacaaaa gaatcgtcgc atggcagttg
      181 gaacagtaca tgcaagagca taatctaaag aaaacggagt tcgcggagag actcggtaca
      241 agccgatcgc aactgaacag acttctggac gccaaagaca cgagcatcaa tctgaacacc
      301 atggcaaaaa tcgcaagggc gatgggaaag acgttcagaa tccagttcga gcagagatcc
      361 gaggagcgtc cgtgagacgg agtggagtac acaatcgcct cacggacaat agtcgcccga
      421 ccttttctac agcgaggagc cgaggccact cagcgaagac ggtcgaggcg gatgcttttc
      481 accagaggca gaacctcccg cggaacgaca aaggagaccg ggagagtgta cttcaagcac
      541 tccacagtcg ttttcttgcg ctcgtcggtg acgatgagcg acggaaaaac cgctctcatt
      601 tcgctactgg tagcccagga ttttttcaga aggatacttt caagctccca tccatttcct
      661 tgtgcggtag aaatacgcaa ccataccgag ccttgagtta aacgttcttc aactttgaaa
      721 aaggctcctt tgtctattcg tttcatctcc atcacccctg atgccgttgt accgggtttt
      781 tctgttaaga gagatgtatt agcatcaaga agataagaat taccaacggt gaactcggtg
      841 ccaggacctc caattttttt tacttctcgt tctaacaaaa aacccagtgg aattttcttc
      901 tcctccgtac taaatacatc atcgcctcct tcagccagca gggtgaagac aaaacgcgtc
      961 atttccgtga agtgggaaga ttccagcttt atatcttcag ggagggaata gagcatgaaa
     1021 gaaaaaagct ccgggcgatc cactaacaga aactcgttcc cctctctcga tccagcaaac
     1081 tcaagatagg cacttctact cgctgtccgg ccgtt
//
LOCUS       DAOTIL010001022         1113 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminobacteriaceae bacterium isolate AMR_MDS_3026
            NODE_157256_length_1113_cov_2.167297, whole genome shotgun
            sequence.
ACCESSION   DAOTIL010001022 DAOTIL010000000
VERSION     DAOTIL010001022.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868050
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminobacteriaceae bacterium (wastewater metagenome)
  ORGANISM  Aminobacteriaceae bacterium
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae.
REFERENCE   1  (bases 1 to 1113)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1113)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:19:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,224
            CDSs (total)                      :: 3,198
            Genes (coding)                    :: 3,184
            CDSs (with protein)               :: 3,184
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1113
                     /organism="Aminobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_157256_length_1113_cov_2.167297"
                     /isolate="AMR_MDS_3026"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:3034609"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..279
                     /gene="rpsQ"
                     /locus_tag="P5201_15360"
     CDS             <1..279
                     /gene="rpsQ"
                     /locus_tag="P5201_15360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015556311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S17"
                     /protein_id="HRV99976.1"
                     /translation="HRKIRTGTVVSDKMEKTVVIRVERFEKHPLYGKRIVRASKYTAH
                     DEKNECRMGDLVTIGETRRLSKTKRWTLLEIVRKAPVFDEVAENGGEA"
     gene            283..651
                     /gene="rplN"
                     /locus_tag="P5201_15365"
     CDS             283..651
                     /gene="rplN"
                     /locus_tag="P5201_15365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007073026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L14"
                     /protein_id="HRV99977.1"
                     /translation="MIQLTTVLNVADNSGAKKLFCIQVLGGSKRKWGTIGDVIVASVR
                     EAIPNSNVAKGTVVKAVIVRTKKEIRRADGSYVRFDDNAAVIIDNNGDPKGTRIFGPV
                     GRELRAKKYMRILSLAPEVV"
     gene            668..994
                     /gene="rplX"
                     /locus_tag="P5201_15370"
     CDS             668..994
                     /gene="rplX"
                     /locus_tag="P5201_15370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008521525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L24"
                     /protein_id="HRV99978.1"
                     /translation="MSKMRLKKGDRVRVISGKDAGKEGKVLRCIRDRDLLVIEGVNVV
                     TKHVKPSQKNPQSGIIKQEAPIYASKVMLVCPSCGAATRVGRGYLENGNKVRMCKACG
                     EIVDQV"
BASE COUNT          305 a          285 c          337 g          186 t
ORIGIN      
        1 caccgcaaga tccggacagg tacggtcgtg agcgacaaga tggagaagac cgtcgtaata
       61 cgggttgaac ggtttgaaaa acaccccctc tacgggaaga gaatcgtcag ggcgagcaaa
      121 tataccgcgc atgacgaaaa gaatgaatgc cggatgggag atctcgtaac aataggcgag
      181 acccgtcgtc tcagcaagac gaagcgctgg acgcttctgg agatcgtacg caaagccccc
      241 gtcttcgacg aggttgcgga gaacggcggg gaggcgtaag tcatgattca gctgactaca
      301 gtcctcaacg tcgccgacaa ctccggtgcg aagaagcttt tctgcatcca ggtactcggc
      361 ggaagcaagc gcaagtgggg cacaataggc gacgtcatcg tcgcctcggt tcgcgaagcg
      421 attccaaaca gcaacgtcgc caagggaact gtcgtcaagg cggtgatcgt gcgcacaaag
      481 aaggaaatac gccgtgcgga cggctcctat gtccgattcg acgacaacgc tgcggtgatc
      541 atcgataaca acggcgaccc caagggcacc cgaattttcg ggcctgtggg cagggagctg
      601 agggcgaaaa agtacatgcg tatcctctcc ctcgcgcccg aagtcgtatg acggaggata
      661 acggataatg agcaagatga gactgaaaaa aggcgaccgt gttcgggtga tctccggaaa
      721 ggatgcaggc aaagaaggca aagtccttcg ctgcatccgc gacagggacc tgctggtcat
      781 cgagggagtg aacgtggtga ctaagcacgt gaaaccctcc cagaagaatc cccagtcggg
      841 cataatcaag caggaagccc cgatatacgc cagcaaggtc atgctcgtat gcccctcctg
      901 cggcgctgca acacgcgtcg gtcgcggcta tctcgagaac gggaacaagg ttcgtatgtg
      961 caaggcctgc ggcgagatcg tggaccaggt gtaaggaggt aagcgtgaat ggcccctcgg
     1021 ctgcttgaaa aatacaaaaa tgacgtctcg ccgaaactgg cgaaggagtt cagttacaaa
     1081 aacgtgatgg aaataccccg gcttaaaaaa gtc
//
LOCUS       DAOTIL010001023         1112 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminobacteriaceae bacterium isolate AMR_MDS_3026
            NODE_157415_length_1112_cov_2.795648, whole genome shotgun
            sequence.
ACCESSION   DAOTIL010001023 DAOTIL010000000
VERSION     DAOTIL010001023.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868050
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminobacteriaceae bacterium (wastewater metagenome)
  ORGANISM  Aminobacteriaceae bacterium
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae.
REFERENCE   1  (bases 1 to 1112)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1112)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:19:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,224
            CDSs (total)                      :: 3,198
            Genes (coding)                    :: 3,184
            CDSs (with protein)               :: 3,184
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1112
                     /organism="Aminobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_157415_length_1112_cov_2.795648"
                     /isolate="AMR_MDS_3026"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:3034609"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>1112
                     /locus_tag="P5201_15375"
     CDS             <1..>1112
                     /locus_tag="P5201_15375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009165978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HRV99979.1"
                     /translation="EALEKYGRDLVAMARAGKLDPVIGRDEEVRRVIRILSRKTKNNP
                     VLIGDPGVGKTAIVEGLAGRIVKGDVPEGLKDRSIFALDMGSLVAGAKFRGEFEERLK
                     AVLNEVKESDGRIILFIDELHTIVGAGAAEGAIDAGNMLKPLLARGELHCIGATTVDE
                     YRKRIEKDAALARRFQPVLVEQPTVEDTVSILRGLKDRFQVHHGVKIRDNALVAAAVL
                     SNRYITERFLPDKAIDLVDEACALIRTEIDSLPAELDAVSRKVMQLEIEEAALNKEKD
                     AASVERLKALQKELQEAREEQTGLRAQYEAEKQGIGVVRELRERIESIRRDIEKAERE
                     YDLNRAAELKHGTLPQLEAELREREKALENGHAGPK"
BASE COUNT          241 a          318 c          394 g          159 t
ORIGIN      
        1 cgaggcgctg gagaagtacg gccgcgatct ggtggcgatg gcccgcgccg gaaagctcga
       61 ccctgtcatc gggcgcgacg aagaggtccg cagggtgatt cgcatcctga gcaggaagac
      121 gaagaacaac cccgtgctta tcggcgaccc cggagtcggc aagacggcca tcgtagaggg
      181 gctcgccggg cgtatcgtga agggcgacgt ccccgagggg ctgaaggacc gttccatctt
      241 cgcgctcgat atggggtcgc tcgtcgcggg ggccaagttc aggggcgagt tcgaggagcg
      301 gttgaaagcg gtgctcaacg aagttaagga gagcgacggc cggatcatcc tcttcatcga
      361 cgaactgcat accatcgttg gcgcgggcgc cgcggaaggc gccatcgacg cggggaacat
      421 gctcaaacct ctgcttgcac gcggcgaact gcactgtatc ggcgcaacca cagtggacga
      481 gtaccgcaag cgcatcgaaa aggacgcggc gctcgccagg cggtttcaac ccgtcctggt
      541 ggagcagccg acggtggagg atactgtttc catattgcgg gggctcaagg accgctttca
      601 ggtgcaccac ggagtgaaga tacgcgacaa cgccctcgtc gcggcggcgg tgctctccaa
      661 ccgctatatc accgagcgat ttctcccgga caaggccatc gacctggtgg acgaggcgtg
      721 cgcgctcatc cgtacggaga tcgactcgct tcccgcggag cttgacgccg tctcgagaaa
      781 ggtgatgcag cttgaaatag aggaggccgc gctcaacaag gagaaggacg cggcgagcgt
      841 cgaacgtttg aaggcgctcc agaaggagct gcaggaggcg cgcgaggagc agaccggcct
      901 gcgcgcgcag tacgaggccg agaagcaggg aattggcgtt gtccgcgagc tgcgggagcg
      961 catcgaatca atcaggcgcg atatcgagaa ggcagagcgc gagtacgacc tgaacagggc
     1021 cgccgagctg aagcacggca cgcttccaca gcttgaggcc gaactgcgcg agcgggagaa
     1081 ggcgctggag aacgggcacg ccggaccaaa ga
//
LOCUS       DAOTIL010001024         1111 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminobacteriaceae bacterium isolate AMR_MDS_3026
            NODE_157721_length_1111_cov_1.437500, whole genome shotgun
            sequence.
ACCESSION   DAOTIL010001024 DAOTIL010000000
VERSION     DAOTIL010001024.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868050
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminobacteriaceae bacterium (wastewater metagenome)
  ORGANISM  Aminobacteriaceae bacterium
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae.
REFERENCE   1  (bases 1 to 1111)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1111)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:19:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,224
            CDSs (total)                      :: 3,198
            Genes (coding)                    :: 3,184
            CDSs (with protein)               :: 3,184
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1111
                     /organism="Aminobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_157721_length_1111_cov_1.437500"
                     /isolate="AMR_MDS_3026"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:3034609"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>1111
                     /locus_tag="P5201_15380"
     CDS             <1..>1111
                     /locus_tag="P5201_15380"
                     /inference="COORDINATES: protein motif:HMM:TIGR03794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NHLP bacteriocin system secretion protein"
                     /protein_id="HRV99980.1"
                     /translation="SPEQLDMLFQVVRPAYWAVLSALLLLICVLVVWGFYGTVTTMVS
                     GQGILLREGARVDASSPGSGRLEAIFVDIDDSAGKGQVIARLAQPDLRHQIAEMRGRV
                     EILRAERAMGTEMGADKKMLESEYLARRRQALNSSIKVARDRAKSLREQLEQYDALYA
                     KKYITRTQYLEVKDRYNAVLQEILSFNEDLARLPMTAADTASQIERQRIDVDMRLLTA
                     EKQLAALEERYDLETSIRSPEAGRVLEIFKLEGQVISAGEALLSLEKNDDTGAPLYVH
                     AYVQPQDGKKILPEMEAQISPSVVKQEEFGVLRGRVTQVSSFPASARGMTRVLGNEQL
                     VQALSRGGAPIAVTMELYPSDKTESGYVWSSRNGPP"
BASE COUNT          206 a          335 c          369 g          201 t
ORIGIN      
        1 tcccccgaac agctcgacat gctctttcag gtcgtccggc ccgcgtactg ggcggtgctc
       61 tccgcgctgc tgcttttaat ctgtgtgctg gtcgtctggg ggttctacgg cacggtgacc
      121 acgatggtct ccgggcaggg cattctgctg cgggagggag cgcgtgtgga cgcctcctct
      181 ccggggagcg gacgccttga ggccatattt gtcgatatcg acgactccgc cgggaagggg
      241 caggtgattg cccggcttgc ccagcccgac cttcggcatc agatcgccga gatgcggggc
      301 agggtggaga ttctgcgtgc agagcgtgcg atgggtacgg agatgggtgc cgacaaaaag
      361 atgctggaat cggaatacct cgcccggcgc agacaggctc tgaactcctc catcaaggtc
      421 gcccgggatc gtgccaagtc gctgcgtgag cagctggagc agtacgatgc cctctacgcg
      481 aaaaaataca taacccgtac ccaatatctc gaggtgaagg atcgctacaa cgctgttctg
      541 caggagatcc tctccttcaa cgaggatctg gcccggctgc cgatgaccgc cgccgatacc
      601 gcctcgcaga tagagcggca gcggattgac gtggatatgc gccttctgac ggcggagaag
      661 cagcttgccg cgctcgagga gcgctacgac ctggagacct ccatccgcag tcccgaggcc
      721 gggcgcgtgc tggagatttt caagctggaa ggtcaggtca tatccgccgg ggaggcgctc
      781 ctctcgctgg agaagaacga tgataccggc gcgcctctgt acgtgcacgc ctatgtccag
      841 ccgcaggacg gcaaaaagat cctgccggag atggaggcgc agatttcccc gtccgtggtg
      901 aagcaggagg agttcggcgt gcttcgcgga cgggtgactc aggtctcctc attccccgcg
      961 tccgcgaggg gaatgacgcg ggtgctggga aacgaacagc tcgtccaggc actttcccgc
     1021 ggaggcgcgc ccatagccgt gacgatggag ctctacccct cggacaagac ggagagcggc
     1081 tacgtctggt cctccaggaa cgggcccccg c
//
LOCUS       DAOTIL010001025         1110 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminobacteriaceae bacterium isolate AMR_MDS_3026
            NODE_157775_length_1110_cov_3.321327, whole genome shotgun
            sequence.
ACCESSION   DAOTIL010001025 DAOTIL010000000
VERSION     DAOTIL010001025.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868050
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminobacteriaceae bacterium (wastewater metagenome)
  ORGANISM  Aminobacteriaceae bacterium
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae.
REFERENCE   1  (bases 1 to 1110)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1110)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:19:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,224
            CDSs (total)                      :: 3,198
            Genes (coding)                    :: 3,184
            CDSs (with protein)               :: 3,184
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1110
                     /organism="Aminobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_157775_length_1110_cov_3.321327"
                     /isolate="AMR_MDS_3026"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:3034609"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            21..>1110
                     /locus_tag="P5201_15385"
     CDS             21..>1110
                     /locus_tag="P5201_15385"
                     /inference="COORDINATES: protein motif:HMM:NF015714.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ComEC/Rec2 family competence protein"
                     /protein_id="HRV99981.1"
                     /translation="MERSWGSRRIVLVDASSGTYLMRLSPNRYILEGDALFFTGRTKA
                     IAPRPGSSFREDLYWEARGVSIEVVPESITRNTGGWSLARWRTALRKRILLTLPYRTR
                     GYLLAAVLGVRDPDLTEAHRRWGTSHLLAVSGFHVGLVAFAVWRFLSFPSLRLVCSRR
                     LAAVLSSLFLWGYVLLAGGAPGALRAALMIQALLLGTVLGRKGTPLNSVSLAALLLLL
                     WRPAWFFDIGWRLSVMAALLLAGLGERRLSLWMLPLSSLFIWFAAFPQVTATFGEVPV
                     AGIALNLVALPLFSLLYPIALFLSLPLLLSVPSGEIFAGTAEGLFSLWEWVADSAASF
                     FPWTLSWSPLLATLGGGLFLLTVTGGLFP"
BASE COUNT          145 a          322 c          326 g          317 t
ORIGIN      
        1 aggacgaagg aacagttgtt ttggaacggt cctgggggag caggcggatt gtgcttgtgg
       61 acgcttcctc tggaacgtac ctgatgaggc tctctccgaa ccggtatatt cttgaagggg
      121 acgcactctt cttcacaggc agaacgaaag cgatcgcacc gcgtcccggt tcgtctttca
      181 gggaagatct ctactgggag gcgaggggcg tctcgatcga ggtcgtcccc gaatcgatta
      241 cacggaatac cggcggatgg tcgctcgccc gctggagaac ggcgctcaga aaacggattc
      301 ttcttacgct gccgtatcgg acgcgggggt acttgcttgc ggcggttctg ggcgtgcgcg
      361 atcctgacct gacggaagcg catagacgct gggggacgtc gcatcttctg gcggtttcag
      421 gttttcatgt cggccttgtg gcgttcgcgg tgtggcgttt tctttccttt ccgtctctac
      481 ggctcgtttg ctcgcgccga ttggccgccg ttctttcaag cctcttcctc tgggggtacg
      541 ttcttctcgc cggaggggcc ccgggcgctc tccgcgctgc actgatgatt caggcgctcc
      601 ttcttggcac tgttctcgga cgaaagggta ctcctttgaa ttcagtctcc cttgctgcgc
      661 tgcttctgct tctctggcgt cctgcctggt ttttcgatat cggctggcgt ctctccgtga
      721 tggccgcgct tcttcttgcg ggcctcgggg agcgccgcct ctcattgtgg atgcttccgc
      781 tttccagtct cttcatttgg tttgccgcgt tccctcaggt gacggccaca ttcggagagg
      841 ttcccgtcgc cggaatagcg cttaatcttg tcgcgctccc tctcttttca ctgctctatc
      901 cgattgcact ttttctttcg cttcctcttt tgctttcagt gccttcaggt gagattttcg
      961 cggggacagc cgagggactt ttttctctct gggagtgggt tgcagacagc gcggcatctt
     1021 tctttccctg gacgctctca tggtctccgc tgcttgcaac gcttgggggc ggcctattcc
     1081 tcttgacagt gacaggagga ctctttcccc
//
LOCUS       DAOTIL010001026         1106 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminobacteriaceae bacterium isolate AMR_MDS_3026
            NODE_158635_length_1106_cov_3.175071, whole genome shotgun
            sequence.
ACCESSION   DAOTIL010001026 DAOTIL010000000
VERSION     DAOTIL010001026.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868050
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminobacteriaceae bacterium (wastewater metagenome)
  ORGANISM  Aminobacteriaceae bacterium
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae.
REFERENCE   1  (bases 1 to 1106)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1106)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:19:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,224
            CDSs (total)                      :: 3,198
            Genes (coding)                    :: 3,184
            CDSs (with protein)               :: 3,184
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1106
                     /organism="Aminobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_158635_length_1106_cov_3.175071"
                     /isolate="AMR_MDS_3026"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:3034609"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..451)
                     /gene="dapF"
                     /locus_tag="P5201_15390"
     CDS             complement(<1..451)
                     /gene="dapF"
                     /locus_tag="P5201_15390"
                     /EC_number="5.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014805952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008837 - diaminopimelate epimerase
                     activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate epimerase"
                     /protein_id="HRV99982.1"
                     /translation="MIPFVKMQGNGNDFIVLDNRDVRFSSDDLFSMARTYCRRRSSLG
                     ADGILVVENSQEADFTMRLFNADGSEGEMCGNGARCIARYAFEKKIAGNEQSFSTLAG
                     IMKAVVTPPFVDLDMGTVPLSSGWFNRPLSVGARSFDTSFLVVGVPHL"
     gene            554..>1106
                     /locus_tag="P5201_15395"
     CDS             554..>1106
                     /locus_tag="P5201_15395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014162452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homocysteine S-methyltransferase family protein"
                     /protein_id="HRV99983.1"
                     /translation="MNKQEFIRWIEEHRAIVPLDGGMGTLLAEKGWTPPMLPEEMNIH
                     SPEVVAGIHAAYINAGVRIIETNTFGGTPIKMAHRGLREKAREINARAARLAKSAAGE
                     RALVAGSVGPLGELLAPFGSLTFDEAYASFRTQMEGLLEGGADFLLIETMIDLREAKA
                     AVLAAKDVNPDGAFVVSFTFDQKG"
BASE COUNT          266 a          313 c          301 g          226 t
ORIGIN      
        1 caaggtgcgg aacgccgacc acgaggaagg atgtatcgaa cgatctagcg cctaccgaga
       61 gcgggcggtt gaaccagccg gaagaaagag gaacagtccc catgtccaga tcgacgaacg
      121 gaggcgttac aaccgctttc ataataccag caagagttga aaacgactgc tcgtttcctg
      181 cgatcttctt ttcgaaagca tatctcgcga tgcagcgggc gccgtttccg cacatctctc
      241 cctcgctgcc gtcggcattg aagagacgca tcgtaaaatc agcctcctgc gaattttcca
      301 caacgagtat cccgtccgct ccgagcgagc ttcgtctgcg gcaatacgtt cgcgccatag
      361 aaaaaagatc atcagatgaa aaacgcacat ccctgttatc caagacaata aaatcgttgc
      421 cgttaccctg catcttcaca aaaggaatca ttgttttccc tcctcctctt ttccttctat
      481 attttacact atccttcttc agttcttttt gctatattga taaacttata cggagcgcag
      541 agggggaaga aaaatgaaca aacaagaatt catccgctgg atcgaagaac accgcgcgat
      601 tgtgccgctt gacggcggta tggggacgct gctcgcggag aaggggtgga cccctccgat
      661 gctgccggag gagatgaaca ttcacagtcc ggaagtagtc gccggcatcc atgccgccta
      721 tataaacgcc ggagtgcgca tcatagagac aaacactttc ggcggaaccc cgataaaaat
      781 ggcccacagg gggcttaggg aaaaggcgcg cgagatcaac gcacgggctg cgcgcctcgc
      841 gaagagcgca gccggagagc gcgcgctcgt cgccggttcc gtcggcccgc ttggcgagct
      901 gttggcgccg ttcggctcac tgaccttcga cgaggcttac gcctctttcc gcacgcagat
      961 ggaggggctt ctggaaggcg gggcggactt tcttctcatc gaaacgatga tcgacctccg
     1021 ggaggccaag gcggctgtcc ttgccgccaa ggacgtaaat cccgacggcg cgtttgtggt
     1081 gagcttcacg ttcgaccaaa agggca
//
LOCUS       DAOTIL010001027         1106 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminobacteriaceae bacterium isolate AMR_MDS_3026
            NODE_158645_length_1106_cov_2.957184, whole genome shotgun
            sequence.
ACCESSION   DAOTIL010001027 DAOTIL010000000
VERSION     DAOTIL010001027.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868050
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminobacteriaceae bacterium (wastewater metagenome)
  ORGANISM  Aminobacteriaceae bacterium
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae.
REFERENCE   1  (bases 1 to 1106)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1106)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:19:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,224
            CDSs (total)                      :: 3,198
            Genes (coding)                    :: 3,184
            CDSs (with protein)               :: 3,184
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1106
                     /organism="Aminobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_158645_length_1106_cov_2.957184"
                     /isolate="AMR_MDS_3026"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:3034609"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..747
                     /locus_tag="P5201_15400"
     CDS             <1..747
                     /locus_tag="P5201_15400"
                     /inference="COORDINATES: protein motif:HMM:NF013660.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRV99984.1"
                     /translation="TSDLVMSLPKLYEAAFTLGEERSTDDYSGDTVFEGRVPNDARAR
                     IEAALPSFLGVRLQLPPGISAVRVGGRRAHAVVRSGEELSLRSRPVFVRSISLLPGEE
                     MKNVFTIRVLCGKGTYIRSIVRDIGRMLGCGAYVLRLIRKQSGLLSLGQALPFEEFQN
                     GDADLRSHIFPLARFAENFYCYKADEERTARLRDGLAVPLSSLRFSHEGILSPDQGAA
                     VLGKTLFCYGRVLKEGLFKPKINIDLEGTE"
     gene            744..>1106
                     /locus_tag="P5201_15405"
     CDS             744..>1106
                     /locus_tag="P5201_15405"
                     /inference="COORDINATES: protein motif:HMM:NF018299.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="riboflavin biosynthesis protein RibF"
                     /protein_id="HRV99985.1"
                     /translation="MIVVLGAFDGFHRGHALLLDRARELSGRHSRSAANGEWAVVTFS
                     PHPDSVVRGGMFSLFSEDERDLLVRFFNVPHIEKIPFDETLSRTSAEGFIALLESLLP
                     VTGVIVGEDFRFGRDREGD"
BASE COUNT          226 a          285 c          306 g          289 t
ORIGIN      
        1 acgagcgatc ttgtgatgtc cctgccaaaa ctctacgagg ccgccttcac gcttggagag
       61 gagcgctcaa ccgacgacta ctccggcgat actgttttcg aagggcgcgt cccgaatgat
      121 gcacgggctc gtatagaggc ggcgctcccg tcatttctcg gagttcggct ccaacttcct
      181 ccgggaatat cagccgttcg agtaggagga agacgggctc atgctgtcgt gcgttcggga
      241 gaagagctct ctcttcgcag ccgacctgtt tttgtacgtt ccatttcgct ccttcccggc
      301 gaagagatga aaaatgtgtt cacaatacgc gttctctgcg ggaaaggaac atatattcgc
      361 agtatcgttc gcgatatagg aagaatgctc gggtgcgggg catacgtcct ccggcttatc
      421 cgaaaacagt ccggattact ctctttgggg caggctctcc cttttgaaga gttccaaaac
      481 ggggatgccg atcttcgatc acacattttc ccgctcgctc gttttgcgga gaatttttac
      541 tgctataaag ccgatgaaga acgaacagcc cgtctgcgcg acggcctcgc agtgccgctt
      601 tcctcccttc gtttttcgca tgaaggtata ctttctcctg accagggagc ggcggtcctg
      661 ggaaaaactc ttttttgcta cggacgagtt cttaaggaag gattgttcaa gccgaaaatc
      721 aatattgatc tggagggaac cgaatgattg tcgtgcttgg agctttcgac ggattccaca
      781 gaggacacgc tcttcttctc gatagagcca gagagctttc cgggcgacat agccgttctg
      841 ctgcaaacgg agagtgggca gtagttactt tttctccgca cccggattcg gttgtccggg
      901 ggggaatgtt ttccctcttt tccgaggatg agcgcgatct tctcgttcgc ttcttcaacg
      961 ttccgcatat tgaaaaaatt ccatttgacg agacgctctc acggacctcg gcggagggat
     1021 tcattgccct gttggagtcg cttcttcctg tgactggagt tatagtggga gaagattttc
     1081 gcttcgggcg cgaccgggag ggggac
//
LOCUS       DAOTIL010001028         1106 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminobacteriaceae bacterium isolate AMR_MDS_3026
            NODE_158683_length_1106_cov_2.509039, whole genome shotgun
            sequence.
ACCESSION   DAOTIL010001028 DAOTIL010000000
VERSION     DAOTIL010001028.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868050
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminobacteriaceae bacterium (wastewater metagenome)
  ORGANISM  Aminobacteriaceae bacterium
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae.
REFERENCE   1  (bases 1 to 1106)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1106)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:19:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,224
            CDSs (total)                      :: 3,198
            Genes (coding)                    :: 3,184
            CDSs (with protein)               :: 3,184
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1106
                     /organism="Aminobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_158683_length_1106_cov_2.509039"
                     /isolate="AMR_MDS_3026"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:3034609"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..499)
                     /locus_tag="P5201_15410"
     CDS             complement(<1..499)
                     /locus_tag="P5201_15410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012737119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal-phosphate dependent enzyme"
                     /protein_id="HRV99986.1"
                     /translation="MKKLSMTFGPFGGQHVPEVLLPALGELEEAFLASREDLSFAEEM
                     THLLRDYVGRPSPLYFAPRITESLGGAKVYLKREDLQPVFSFKLRGAYAKLVAMGESG
                     RGRGVIAASAGNHAQGVALAASRLGTRAVIVMPVTTPSIKVAAVRSRGGEVVLHGDTF
                     DEARRH"
     gene            complement(578..>1106)
                     /gene="trpA"
                     /locus_tag="P5201_15415"
     CDS             complement(578..>1106)
                     /gene="trpA"
                     /locus_tag="P5201_15415"
                     /EC_number="4.2.1.20"
                     /inference="COORDINATES: protein motif:HMM:TIGR00262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004834 - tryptophan synthase activity
                     [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="tryptophan synthase subunit alpha"
                     /protein_id="HRV99987.1"
                     /translation="PRVLMGYMNSVLAYGQEKFAADAAGAGVSGVIIPDLPFHQGEKL
                     ASLLREQGVDFILMVTPNTPAERLRGICERASGFLYCVSRLGITGESGTAGASPKEYI
                     DRIRSHTSLPLALGFGVGTPEKAALAAEAADGVVVGSALISLLEPCGTDRELLRSKAV
                     PFLRSLREAVGRTRR"
BASE COUNT          180 a          367 c          384 g          175 t
ORIGIN      
        1 cgtggcggcg cgcctcgtcg aaggtgtcgc cgtgcaggac cacctcgccg ccgcgcgagc
       61 gcaccgcggc gaccttgatg ctcggcgtcg tcaccggcat cacgatcacc gcgcgcgtcc
      121 cgaggcgcga ggccgcgagg gcgacgccct gcgcgtggtt cccggccgac gccgcgatga
      181 cgccgcgccc gcgccccgac tcgcccatcg cgacgagctt cgcgtaggcg ccgcgcagct
      241 tgaacgagaa gaccggctgc aggtcttccc gcttgaggta caccttcgct cctccgagag
      301 attcggtgat gcggggagcg aaatacaggg gcgagggccg gcccacgtag tcccgcagca
      361 gatgggtcat ttcttcagcg aaggacaggt cctcccggct cgcgagaaag gcttcctcca
      421 gttcgccaag ggcgggaaga aggacttccg gaacatgctg tcccccgaag gggccgaagg
      481 tcatggataa ctttttcatg gcagatctct cctttttgta tgcggaaatt tgaacttctc
      541 ctgccggaag ggcttcaagg ccgttccgga ggactgttta cctccgtgtc cgccccaccg
      601 cctcccggag ggaacggagg aaggggacgg ccttcgaccg aaggagctcc ctgtcggtgc
      661 cgcagggctc aaggagggag atgagggcgc tgcccaccac cacgccgtcc gccgcctcgg
      721 cggccagggc ggccttttcg ggggtgccca cgccgaagcc cagggccagg ggcagggagg
      781 tgtgggaacg gattcggtcg atgtactcct tcggggacgc tcccgccgtt ccgctttccc
      841 cggtgatgcc cagtcgggag acgcagtaga ggaacccgga cgcccgttcg cagatgcccc
      901 gcagccgttc ggcaggggtg ttgggggtga ccatgaggat gaagtccacc ccctgctccc
      961 gaaggaggga agcgagcttc tctccctggt ggaagggcag gtcggggatg atgacccccg
     1021 acactcccgc cccggcggcg tcggcggcga acttctcctg cccgtaggcg aggacggagt
     1081 tcatgtaccc catgagcacc cggggg
//
LOCUS       DAOTIL010001029         1105 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminobacteriaceae bacterium isolate AMR_MDS_3026
            NODE_158856_length_1105_cov_2.997143, whole genome shotgun
            sequence.
ACCESSION   DAOTIL010001029 DAOTIL010000000
VERSION     DAOTIL010001029.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868050
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminobacteriaceae bacterium (wastewater metagenome)
  ORGANISM  Aminobacteriaceae bacterium
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae.
REFERENCE   1  (bases 1 to 1105)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1105)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:19:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,224
            CDSs (total)                      :: 3,198
            Genes (coding)                    :: 3,184
            CDSs (with protein)               :: 3,184
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1105
                     /organism="Aminobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_158856_length_1105_cov_2.997143"
                     /isolate="AMR_MDS_3026"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:3034609"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..471)
                     /locus_tag="P5201_15420"
     CDS             complement(<1..471)
                     /locus_tag="P5201_15420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009202426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytosine deaminase"
                     /protein_id="HRV99988.1"
                     /translation="MAFSLLIKNARTRFSQGKTLSIGIEGEKIAAVGENLPETGAAQI
                     IDAGGKLVTESFVNGHLHLCKVYTLEMVGQDALSSYHGGSMGGAMTAIEQASRVKDHY
                     DEKWIIENVRKACRLAQKYGNTHIRAFADTDTKAKLEGIKALLKAREEFKNIVDL"
     gene            complement(527..1003)
                     /locus_tag="P5201_15425"
     CDS             complement(527..1003)
                     /locus_tag="P5201_15425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006584004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(2Fe-2S)-binding protein"
                     /protein_id="HRV99989.1"
                     /translation="MRVLNFTVNGTPRTVHLEEGRVMLASVLRDSLGLTGTKVGCGTG
                     QCGSCTVVMDGKAVTSCTVPAEKAEGTSVLTIESLAEGGVLHPIQQAFVEAGAIQCGF
                     CTPGIVMRLYALFTQNPDADDETIMKELDKHLCRCTGYENIVKAAHLAQEKLRAAR"
BASE COUNT          154 a          360 c          345 g          246 t
ORIGIN      
        1 gaggtccacg atgttcttga actcctcacg ggccttcagc agcgccttga tcccctccag
       61 cttcgccttg gtgtcggtgt cggcgaaggc gcggatatgg gtgttgccgt atttttgggc
      121 caggcggcac gccttgcgga cgttctcgat gatccacttc tcgtcgtagt ggtccttcac
      181 gcgcgaggcc tgctcgatgg ccgtcatggc cccgcccatg ctccctccgt ggtaggagga
      241 gagcgcgtcc tgccccacca tctccagcgt gtacaccttg cacaggtgga gatggccgtt
      301 cacgaaggac tccgtgacca gcttcccgcc cgcgtcgatg atctgcgcag cgcccgtctc
      361 cgggaggttc tcgccgacgg cggcgatctt ctccccctcg atgccgatgg agagggtctt
      421 tccctgtgaa aagcgggtcc gtgcgttctt gatcagaaga ctgaatgcca tgagttcatt
      481 ctcctttcat tttttgctgc ctgaaagcgt tccgtcgcca gcccgctcac cgtgccgcgc
      541 ggagcttctc ctgcgcgagg tgcgccgcct tgacgatgtt ctcgtagccg gtgcagcggc
      601 agaggtgctt gtcgagctct ttcatgatcg tttcgtcgtc ggcgtccgga ttctgggtga
      661 agagcgcgta gaggcgcatg acgattcccg gcgtgcagaa gccgcactgg atggccccgg
      721 cctcgacgaa ggcctgctgg atcgggtgga ggacgccgcc ctcggcgagg gactcgatgg
      781 tgaggacgct cgtcccttcc gccttttccg cggggacggt gcagctggtc acggccttcc
      841 cgtccatcac cacggtgcac gagccgcact gccccgtgcc gcaccccacc ttggttcccg
      901 tcagcccgag cgagtcgcgc agcacggagg cgagcatcac gcgcccctct tcaagatgga
      961 ccgtccgggg tgttccgttg actgtgaagt tgaggacgcg catgctattt gtcctccttt
     1021 cgggttttca gggcttccag tattctctcc ggcgtgaccg gaatcgctgt gaactcgacg
     1081 cccgtcgcgt cgcgcagcgc gttga
//
LOCUS       DAOTIL010001030         1102 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminobacteriaceae bacterium isolate AMR_MDS_3026
            NODE_159466_length_1102_cov_3.497612, whole genome shotgun
            sequence.
ACCESSION   DAOTIL010001030 DAOTIL010000000
VERSION     DAOTIL010001030.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868050
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminobacteriaceae bacterium (wastewater metagenome)
  ORGANISM  Aminobacteriaceae bacterium
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae.
REFERENCE   1  (bases 1 to 1102)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1102)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:19:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,224
            CDSs (total)                      :: 3,198
            Genes (coding)                    :: 3,184
            CDSs (with protein)               :: 3,184
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1102
                     /organism="Aminobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_159466_length_1102_cov_3.497612"
                     /isolate="AMR_MDS_3026"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:3034609"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..909)
                     /locus_tag="P5201_15430"
     CDS             complement(<1..909)
                     /locus_tag="P5201_15430"
                     /inference="COORDINATES: protein motif:HMM:NF017346.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate-utilizing N-terminal
                     domain-containing protein"
                     /protein_id="HRV99990.1"
                     /translation="MRGIGVSPGIAIGKAVIHWKEQIDIVREYVDEPERELRRFRTAL
                     EFAGDQIRESYSRVLQNVGPNEAAIFEAHGLMLQDPDFTGRIERLILDESANAEWAVK
                     QVSDNLVQIFENMDNDYMKARSDDVRDISERVIKLLLQAGGMDFTQLQEKAILVTRDF
                     TASDMSQIQREMTLGVASEVGGRTSHAAIIARTMQIPSVMGAHGLLSTVGNGDLLILD
                     GETGEIFVNPDEETLARYKEKQERYECFLGRLSEIRGKETVTLDGVKVELGANVGSSR
                     DLDEVLKNDGEAVGLYRTEFLYLESPM"
BASE COUNT          201 a          331 c          329 g          241 t
ORIGIN      
        1 catcgggctt tcgaggtaga ggaactccgt tcgatagaga ccgacggcct cgccgtcgtt
       61 cttcagcacc tcgtcgagat cgcgggaaga accgacgttt gccccaagct cgaccttcac
      121 gccgtcgagc gtgacggtct ctttcccgcg gatttcggag aggcgtccga ggaagcattc
      181 gtaccgttcc tgcttttcct tgtagcgcgc cagcgtctcc tcgtcggggt tcacgaatat
      241 ctcccccgtc tcgccgtcga ggatgagcag gtcgccgttg cctaccgtgc tcagcagccc
      301 gtgcgccccc atcacggagg ggatttgcat cgtgcgggcg atgatcgccg catgagaggt
      361 cctgcctccg acttcggagg cgacgccgag cgtcatctcg cgctggatct gcgacatgtc
      421 ggacgcggtg aagtcgcggg taacgaggat ggccttctcc tgaagctgcg tgaagtccat
      481 tcctcccgcc tgaaggagga gctttatcac ccgttcggag atgtccctga catcgtcgga
      541 gcgggctttc atgtagtcgt tgtccatgtt ctcgaagatc tggacgaggt tgtccgaaac
      601 ctgtttcacc gcccactcgg cgttcgcgct ttcgtcgagg ataaggcgct ctatccgtcc
      661 ggtgaagtcg gggtcctgca gcatcaggcc gtgcgcctcg aagatggcgg cctcgttggg
      721 gccgacgttc tgcagtaccc gcgaatatga ttcgcgaatc tgatctccag cgaattcgag
      781 cgcggtgcga aaacggcgaa gttcccgctc gggttcgtcg acgtactccc gaacaatgtc
      841 gatctgctct ttccagtgaa tgaccgcttt gccgatggcg attccgggag ataccccgat
      901 tcctctcatg gttttcagca cctgcctgtc gaatgtgtat cacataatta tacacgcagt
      961 tcggcctttg tgcgcaaagg agtgaacaag cgtttcgtcc tccggaagaa tggaaatccg
     1021 ccgtgcggga agcgacgcgc ttacaagatc gacagcagca cgtgaaggag cggcagcagc
     1081 cccgccatgc cgagcacgga ga
//
LOCUS       DAOTIL010001031         1101 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminobacteriaceae bacterium isolate AMR_MDS_3026
            NODE_159664_length_1101_cov_4.478967, whole genome shotgun
            sequence.
ACCESSION   DAOTIL010001031 DAOTIL010000000
VERSION     DAOTIL010001031.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868050
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminobacteriaceae bacterium (wastewater metagenome)
  ORGANISM  Aminobacteriaceae bacterium
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae.
REFERENCE   1  (bases 1 to 1101)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1101)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:19:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,224
            CDSs (total)                      :: 3,198
            Genes (coding)                    :: 3,184
            CDSs (with protein)               :: 3,184
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1101
                     /organism="Aminobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_159664_length_1101_cov_4.478967"
                     /isolate="AMR_MDS_3026"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:3034609"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(244..741)
                     /locus_tag="P5201_15435"
     CDS             complement(244..741)
                     /locus_tag="P5201_15435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008520857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenosine-specific kinase"
                     /protein_id="HRV99991.1"
                     /translation="MPEIQKWELVQLDFPSDCNIILGQSHFIKTVEDLFEALATSSPA
                     LQFGIAFCEASGDCLIRFDGNNEELVKIAVDNMNSLGAGHSFLIVLKNGYPINVLNRI
                     KECQEVCRVFAATANPIQVIICESEQGRGVAGVIDGFSPKGVETESDIEGRKTLLRRI
                     IGYKR"
     gene            complement(788..>1101)
                     /locus_tag="P5201_15440"
     CDS             complement(788..>1101)
                     /locus_tag="P5201_15440"
                     /inference="COORDINATES: protein motif:HMM:NF019887.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="HAD hydrolase family protein"
                     /protein_id="HRV99992.1"
                     /translation="VQAGEGFLDVLPLGVSKGAALRGWLATLPELPEIVVAAGDHHND
                     MEMLKEADIAVVPEDAADEVLALSDVIMPSADKDGFVFLADWLLKGDIHRDENASPVV
                     L"
BASE COUNT          247 a          331 c          297 g          226 t
ORIGIN      
        1 cgtcatccgg cgggagaagc gcaacctgcc atatccaccc gtcctccacg ccgacgatca
       61 ggtcgttggg tgcggaaagg gcgggaagcg caggtacgaa cagcaaaaag agcaccaaaa
      121 gaacagggat gcgcagaagg ctcacggaga gaggagaacg accggtcccg catacaggcc
      181 ttctcttcgt caggcccttc cgaccgtccg aggcgcacgc tctccgagat tgatgcgcga
      241 ccattaccgt ttgtagccga ttatccggcg aagcagtgtt tttctcccct cgatgtcgct
      301 ttcggtctcc acgcccttgg gagagaagcc gtcgatgacg cccgccacgc ctctcccctg
      361 ttcgctttca cagatgatga cctgaatggg attggccgtg gccgcaaaga cgcggcagac
      421 ttcctgacac tccttgatcc tgttgagtac attgatcggg tacccgttct tgaggacgat
      481 gagaaacgaa tgaccagctc ccagagagtt catgttgtcc acggctattt tgacgagctc
      541 ttcattattg ccgtcgaacc gtatgaggca gtctccggag gcttcgcaga aagcgatacc
      601 gaactgcagc gccggagagg aggtcgcaag ggcttcgaac aggtcctcga cggttttgat
      661 aaaatggctc tgtccgagaa taatattgca gtcgctcgga aaatcaagct gcacaagttc
      721 ccacttctga atttcaggca tgaattgtcc ctccctcttc ttttcagggg taattgtagc
      781 tgcctatcta taatacaaca ggagatgcgt tttcgtcacg gtgaatatct cccttgagaa
      841 gccagtccgc gaggaagaca aagccgtcct tgtccgccga cggcatgatc acatccgaaa
      901 gcgcaagcac ctcgtccgcc gcgtcctccg gcacgacggc gatatcggcc tctttcagca
      961 tctccatgtc gttatggtga tctccggccg cgacgacgat ctccggaagc tccggcagcg
     1021 tcgcgagcca tccgcgaagg gccgccccct ttgaaacgcc gagaggaaga acatcgagaa
     1081 acccctcccc cgcctgcacg a
//
LOCUS       DAOTIL010001032         1101 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminobacteriaceae bacterium isolate AMR_MDS_3026
            NODE_159685_length_1101_cov_3.609943, whole genome shotgun
            sequence.
ACCESSION   DAOTIL010001032 DAOTIL010000000
VERSION     DAOTIL010001032.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868050
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminobacteriaceae bacterium (wastewater metagenome)
  ORGANISM  Aminobacteriaceae bacterium
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae.
REFERENCE   1  (bases 1 to 1101)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1101)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:19:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,224
            CDSs (total)                      :: 3,198
            Genes (coding)                    :: 3,184
            CDSs (with protein)               :: 3,184
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1101
                     /organism="Aminobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_159685_length_1101_cov_3.609943"
                     /isolate="AMR_MDS_3026"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:3034609"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..216)
                     /locus_tag="P5201_15445"
     CDS             complement(<1..216)
                     /locus_tag="P5201_15445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008521146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhdT family protein"
                     /protein_id="HRV99993.1"
                     /translation="MTERTESQVLETNEYGFVEDPRYKQCEKEAWYGIILGIVNLVIW
                     AVAGYGLGSGPVEEYSYIMGFPAWFFIS"
     gene            complement(257..1075)
                     /gene="panB"
                     /locus_tag="P5201_15450"
     CDS             complement(257..1075)
                     /gene="panB"
                     /locus_tag="P5201_15450"
                     /EC_number="2.1.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014807411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003864 - 3-methyl-2-oxobutanoate
                     hydroxymethyltransferase activity [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-methyl-2-oxobutanoate
                     hydroxymethyltransferase"
                     /protein_id="HRV99994.1"
                     /translation="MSVLDFKKYKEEGRKFAYVTAYDYTTASIVNESEVEVILVGDSL
                     GMIMLGYKDTVAVTMEDMIHHTRPVVKGAPDTFVVGDMPFGSYNKSIEQGVENATRLV
                     KETGCDCVKLEGGVKYAPLVRAIVDAGISVMGHIGLTPQNATQLGGFKVQGGTPESAR
                     QLIEDAKELEKAGAFSIVLECVPKVVGKAVSEAVKIPILGIGAGPFVDCQVLVTQDLL
                     GMYGDFKPKFVKLYANVRKVMVDGLNQFHKEALDGEFPSDEYSFNKEVNLEKLY"
BASE COUNT          199 a          362 c          280 g          260 t
ORIGIN      
        1 gctgatgaaa aaccacgccg ggaagcccat gatgtaggag tactcctcca ccggaccgct
       61 cccgagcccg tagcctgcaa cagcccatat aacgaggttg acgatcccga gtatgatgcc
      121 gtaccaggcc tctttttcac actgtttgta acgagggtct tcgacaaagc cgtattcgtt
      181 cgtttcaaga acctgcgatt ccgttctttc cgtcatggtc ttccacgacc tccttcctgc
      241 ttctgccggc agatgcctag tacaacttct ccagattcac ctctttgttg aagctgtact
      301 cgtcgctcgg gaactctccg tcgagcgcct ctttatggaa ctggttcagt ccgtccacca
      361 tgaccttgcg gacgttggcg tacagcttga cgaatttcgg cttgaagtcg ccgtacatgc
      421 ccagcagatc ctgtgtgaca agcacctggc agtccacgaa ggggcctgcg ccgatgccga
      481 ggatgggaat cttcaccgct tcggagacgg ccttgccgac aaccttcgga acgcactcca
      541 gcacgatgga aaaggcgccc gccttctcaa gctccttcgc gtcctcgatg agctgccgcg
      601 cgctctccgg cgttccgccc tgcaccttga accccccgag ctgcgtggcg ttctgcggcg
      661 tgaggccgat gtgccccatg accgaaattc ccgcgtccac gatggcccgc acaagaggcg
      721 cgtacttgac gccgccttcc agcttcacgc agtcgcaccc cgtctccttg accaacctcg
      781 tcgcgttttc cacgccctgt tcgatgctct tgttgtacga gccgaacggc atgtcgccca
      841 cgacgaacgt gtcgggcgcg cctttgacga cgggtctggt atggtgaatc atgtcctcca
      901 tggttaccgc cacagtgtcc ttgtacccca gcatgatcat gccgagggag tcgccgacga
      961 ggatgacctc gacctcgctc tcgttgacga tggacgccgt ggtgtagtcg taggccgtca
     1021 cgtaggcgaa ctttctgcct tcctctttgt acttcttgaa atcgagcacc gacatcttct
     1081 ttttcataaa ttgccctcct c
//
LOCUS       DAOTIL010001033         1101 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminobacteriaceae bacterium isolate AMR_MDS_3026
            NODE_159693_length_1101_cov_3.307839, whole genome shotgun
            sequence.
ACCESSION   DAOTIL010001033 DAOTIL010000000
VERSION     DAOTIL010001033.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868050
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminobacteriaceae bacterium (wastewater metagenome)
  ORGANISM  Aminobacteriaceae bacterium
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae.
REFERENCE   1  (bases 1 to 1101)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1101)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:19:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,224
            CDSs (total)                      :: 3,198
            Genes (coding)                    :: 3,184
            CDSs (with protein)               :: 3,184
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1101
                     /organism="Aminobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_159693_length_1101_cov_3.307839"
                     /isolate="AMR_MDS_3026"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:3034609"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..882)
                     /locus_tag="P5201_15455"
     CDS             complement(<1..882)
                     /locus_tag="P5201_15455"
                     /inference="COORDINATES: protein motif:HMM:NF012470.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase"
                     /protein_id="HRV99995.1"
                     /translation="MNRFLRTIGLILLFLLLFAAAAFGGNERAKYVFLFIGDGMGEAQ
                     VEAAELYSVASGNGPLALRGLAVRGKISTRSANAAVTDSAAAGTALATGFKTNNGVLG
                     MDPSGMKKLPTVAEVARDRGMKVGVVTSVFLNDATPAAFYAARPSRTDFYEIGVQMVE
                     SGFDYFAGGGVNRRAGRKGDLKDVYDLAREKGYRVLRTRAELAAARPGTKIIAAASVG
                     GALPYETDRPAGSPSLAEFTKRGIELLDNPNGFFMMVEGGRIDWACHRNDATAAIRDV
                     LAFDEAVKEALIFARARP"
     gene            complement(914..>1101)
                     /locus_tag="P5201_15460"
     CDS             complement(914..>1101)
                     /locus_tag="P5201_15460"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRV99996.1"
                     /translation="RRGGRTGGGNMSRFLFARESLLENIEDARDLAAYAVWLKSARET
                     VRFEELVSECGFSEEDL"
BASE COUNT          193 a          384 c          337 g          187 t
ORIGIN      
        1 cgggcgggca cgggcgaaga tgagcgcctc cttcaccgcc tcgtcgaagg ccagcacgtc
       61 gcggatggcg gccgttgcgt cgttgcggtg gcaggcccag tcgatccttc ccccctcgac
      121 catcatgaaa aagccgttcg ggttgtcgag cagctcgata ccccgcttcg tgaactccgc
      181 aagggaaggg ctccccgcag ggcggtccgt ctcgtacggc agcgccccgc cgaccgacgc
      241 ggccgcgatg atcttcgtcc ccggcctcgc cgcggcaagc tccgcgcggg tgcggagcac
      301 gcggtacccc ttctcgcggg cgaggtcgta cacgtccttc aggtcgccct ttcgccccgc
      361 gcgccggttg acgccgcccc ccgcgaagta gtcgaagccg ctctcgacca tctggacgcc
      421 gatctcgtaa aagtcggtcc gggagggacg cgcggcgtag aaggccgccg gagtggcgtc
      481 gttcagaaag accgacgtga ccacgccgac cttcatcccc cggtcgcggg cgacttccgc
      541 gacggtgggc agttttttca tccccgaagg atccatgccg aggacgccgt tgttcgtctt
      601 gaatccggtg gccagcgccg tcccggcggc ggcggagtcc gtcacggcgg cgttcgccga
      661 ccgcgtggag atcttcccgc gcacggcaag tccgcgcagc gcgagcggcc cgtttccgga
      721 cgcgacgctg tacagctcag cggcctcgac ctgcgcctcc cccatgccgt cgccgatgaa
      781 gagaaagacg tacttcgccc gctcgttgcc tccaaaggcc gcggcggcga agaggagcag
      841 gaagagcagg atcagtccga tagtgcgtaa aaagcggttc atttcatgtt cctcgaatga
      901 cgactaagaa tgctcataaa tcctcctcgg aaaaaccgca ctcgcttaca agctcttcga
      961 agcgcaccgt ttcccgcgcg ctcttcagcc acaccgcata tgcggcaaga tcgcgggcat
     1021 cttcgatgtt ttcaagaagg gactcgcgag caaagagaaa tctactcata tttcctcccc
     1081 cggtacgacc tccgcgacgc c
//
LOCUS       DAOTIL010001034         1101 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminobacteriaceae bacterium isolate AMR_MDS_3026
            NODE_159710_length_1101_cov_3.038241, whole genome shotgun
            sequence.
ACCESSION   DAOTIL010001034 DAOTIL010000000
VERSION     DAOTIL010001034.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868050
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminobacteriaceae bacterium (wastewater metagenome)
  ORGANISM  Aminobacteriaceae bacterium
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae.
REFERENCE   1  (bases 1 to 1101)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1101)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:19:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,224
            CDSs (total)                      :: 3,198
            Genes (coding)                    :: 3,184
            CDSs (with protein)               :: 3,184
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1101
                     /organism="Aminobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_159710_length_1101_cov_3.038241"
                     /isolate="AMR_MDS_3026"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:3034609"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..507
                     /locus_tag="P5201_15465"
     CDS             <1..507
                     /locus_tag="P5201_15465"
                     /inference="COORDINATES: protein motif:HMM:NF012382.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HRV99997.1"
                     /translation="TFSKVLFPGIRVGWAAAHPECIRRLTALRRYGELAPPGLLQAAL
                     ARFCSEGWYEKHISMMHRVYRKRMQTALAALERFVSPEWASWTKPAGGYLIWLSMAPH
                     PPVDWEKVFASEGVAVASGRSFFSSDPAGTFFRLSISSLNEAEIEEGIRRLGKAFQKV
                     YDRKDIAK"
     gene            504..>1101
                     /locus_tag="P5201_15470"
     CDS             504..>1101
                     /locus_tag="P5201_15470"
                     /inference="COORDINATES: protein motif:HMM:NF012428.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class III-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HRV99998.1"
                     /translation="MKLWNTNPVHGIRLTGGKGSALYDDQGRTYIDMFSGTWCNVLGY
                     GHPRLRRAVEEQAQGLFQTGAAFGTRQVEEGLRQLASILPPELERAVFLNSGSEAVEL
                     ALKMAHAATGKTGVVVAEKGYYGGTVYALSLSEAGRTASYLPKAGNVLRVAAPHCVRC
                     DLSAECGDGEFPCMNVLEDLSPEEWPAAILYEPLLGGGI"
BASE COUNT          207 a          294 c          359 g          241 t
ORIGIN      
        1 acgttttcga aggttctttt cccgggaata cgcgtggggt gggcagcggc gcatccggag
       61 tgtatccggc gcttgacagc gcttcgccgg tacggtgaac ttgcgcctcc ggggctgtta
      121 caggctgcac ttgcccgatt ctgttccgaa ggatggtatg agaagcatat ctcgatgatg
      181 cacagggttt atcgaaagcg aatgcagact gcgctggcgg cccttgagcg tttcgtctcc
      241 ccggagtggg cgagttggac aaaacccgcg ggagggtacc ttatatggct gtcgatggct
      301 ccccatcctc ccgtcgactg ggagaaggtt tttgcgtccg agggggttgc cgtcgcttcg
      361 ggacggtcgt ttttttcgtc ggatccggcc ggtacgttct tccgcctctc catttcttcg
      421 ctgaacgagg ccgagataga agaggggata cgcaggctcg ggaaagcgtt tcagaaagtc
      481 tatgacagaa aggatattgc gaagtgaagc tttggaatac caaccccgtg cacggcatcc
      541 gtcttaccgg ggggaaaggg agtgcgctct acgacgatca ggggagaacg tatatcgata
      601 tgttctcggg aacgtggtgc aacgtactcg ggtatggcca cccccgcttg cgtcgcgcag
      661 tcgaggagca ggcgcagggg cttttccaga caggggccgc tttcggcacg cgacaggtag
      721 aggaaggcct ccggcagctt gcttcgattc ttcctccgga actcgaaagg gcggtttttc
      781 ttaattccgg aagcgaagcc gtggagcttg ccctcaaaat ggcgcacgcc gcgacaggaa
      841 agactggagt cgtcgtcgcg gagaaggggt attacggagg cacagtttac gcgctctcgc
      901 tctccgaagc tgggcggacc gcttcatatc tgccaaaagc aggaaacgtc ctccgcgtcg
      961 cggcgccgca ctgcgtccgg tgcgatctct cggcggagtg cggcgatggc gagtttccct
     1021 gtatgaatgt tcttgaggat ctgtcgcctg aagaatggcc tgcggcgatt ctctacgaac
     1081 cgctcctcgg aggcggcatc c
//
LOCUS       DAOTIL010001035         1101 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminobacteriaceae bacterium isolate AMR_MDS_3026
            NODE_159766_length_1101_cov_2.264818, whole genome shotgun
            sequence.
ACCESSION   DAOTIL010001035 DAOTIL010000000
VERSION     DAOTIL010001035.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868050
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminobacteriaceae bacterium (wastewater metagenome)
  ORGANISM  Aminobacteriaceae bacterium
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae.
REFERENCE   1  (bases 1 to 1101)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1101)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:19:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,224
            CDSs (total)                      :: 3,198
            Genes (coding)                    :: 3,184
            CDSs (with protein)               :: 3,184
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1101
                     /organism="Aminobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_159766_length_1101_cov_2.264818"
                     /isolate="AMR_MDS_3026"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:3034609"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>1101
                     /locus_tag="P5201_15475"
     CDS             <1..>1101
                     /locus_tag="P5201_15475"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRV99999.1"
                     /translation="DLYFFGEPLEMIVGDVEPPGVFLGASAVLERQFAAFCLDCWVRE
                     GTTVKDLPEKTSTVLNQLDGKEKNGFPYSFLNFVNRGRTALLDRFFALFRGEISDDVK
                     EHIEHFASSSEEGIAFRLLEGLRRMQKQRASLQHKAKALRKALGKLKEDPAAKPEEVE
                     ALSREKRALERLFRDLGELPLFNLLTDEGLIPNYAFPESGVVLRSVIYRRLEDGKLES
                     DIFKYERAAGAALRELAPSSTFYAGGRKVKIEQVDMSLSEVEEWRFCDVCSYMERVGA
                     EERAGKCPRCGSSGFGDLGQRRKLLRMKQVYAFGDDKSTLIDDDSDEREPAFYVDQTL
                     VNFDEKDVGKAWRSEDTETPFGFEYISRAVFR"
BASE COUNT          221 a          295 c          372 g          213 t
ORIGIN      
        1 cgatctctat ttcttcggcg agcccctcga gatgatcgtg ggcgacgtgg agccccccgg
       61 agttttcctg ggagcctccg ccgtcctcga gcgccagttc gcggcgttct gcctcgattg
      121 ctgggtcagg gagggaacca cggtaaagga tcttccggaa aaaacgtcca cggttctcaa
      181 ccagctggac gggaaggaaa agaacggctt cccctacagt ttcctgaact ttgtcaacag
      241 ggggcggact gccctcctgg accgtttctt cgcccttttc aggggggaga tttccgacga
      301 cgtgaaggaa catattgaac atttcgcgtc ctcttcggag gaagggatcg cctttcggct
      361 gctggagggg ctccgcagga tgcagaagca gagggcctcc ctgcagcaca aggcgaaggc
      421 gctgcggaaa gcgctgggga agctgaagga ggaccctgcc gcgaagccgg aggaggtcga
      481 ggcgctgtcg agggagaaac gcgccctgga acggctcttc cgcgacctcg gcgagcttcc
      541 tcttttcaac ctgctcacgg acgaggggct gataccgaac tacgcctttc ccgagagcgg
      601 cgtcgtgctc cgctccgtca tctacaggag actggaggac ggaaaactcg agagcgatat
      661 tttcaaatac gagcgtgcgg cgggcgccgc ccttcgggag ctggcgcctt cgagcacctt
      721 ttacgccgga gggcgcaagg tgaagatcga gcaggtggat atgtcgcttt cggaggtgga
      781 ggagtggcgt ttctgcgacg tctgctcgta catggagaga gtgggtgcgg aggaacgtgc
      841 ggggaagtgt ccccggtgcg gcagttccgg attcggcgat ctcgggcaga gaagaaagct
      901 gcttcgaatg aagcaggtct acgctttcgg ggacgataag agcaccctga tcgacgacga
      961 cagcgacgag cgcgagccgg ccttctacgt tgatcagacg cttgtgaatt tcgacgaaaa
     1021 agatgtggga aaggcgtggc gctcggagga taccgagact cccttcggct tcgagtacat
     1081 ttcgagggcc gttttccggg a
//
LOCUS       DAOTIL010001036         1100 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminobacteriaceae bacterium isolate AMR_MDS_3026
            NODE_159904_length_1100_cov_3.501435, whole genome shotgun
            sequence.
ACCESSION   DAOTIL010001036 DAOTIL010000000
VERSION     DAOTIL010001036.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868050
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminobacteriaceae bacterium (wastewater metagenome)
  ORGANISM  Aminobacteriaceae bacterium
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae.
REFERENCE   1  (bases 1 to 1100)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1100)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:19:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,224
            CDSs (total)                      :: 3,198
            Genes (coding)                    :: 3,184
            CDSs (with protein)               :: 3,184
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1100
                     /organism="Aminobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_159904_length_1100_cov_3.501435"
                     /isolate="AMR_MDS_3026"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:3034609"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..940
                     /locus_tag="P5201_15480"
     CDS             <1..940
                     /locus_tag="P5201_15480"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HRW00001.1"
                     /translation="FAELMGGSIGVTSEEGKGSEFWFTVRLEKQPEGACRDEVHRHAA
                     ATAPRVERRGVRVLLAEDNAVNRKVALGMLKKLGFTADSVADGEQVLSALSERTYDLV
                     LMDCQMPVMDGYEAARRIRALEKPGGKRIPVVAMTAYAMSGDRERCIEAGMDDYLAKP
                     ISIEALSAALASWLPHREGPGGTDGSPRRSEPPTPREEAETCSKSAVWRKDELLNRLL
                     GDEEMTREILEAFLEDIPREIDSLTAAVAVGNTPTVELLAHTIKGAAGNVGSEGLRSA
                     AFEMESAAKKGDLSGAQALLKSIRQEFEHLKAAIRG"
BASE COUNT          229 a          317 c          394 g          160 t
ORIGIN      
        1 gttcgccgag ctgatgggag gctccatcgg ggtaacgagc gaagagggga aggggtcgga
       61 gttctggttc acggtccgtt tggagaagca gccggaaggc gcctgccgcg acgaggtcca
      121 ccgccatgcc gccgcgaccg ccccgcgcgt ggagagaagg ggcgtgcgcg tcctgctcgc
      181 ggaggacaac gccgtgaaca gaaaagtcgc cctggggatg ctgaaaaagc tcgggttcac
      241 agccgactcc gtcgcggacg gagaacaggt tctctccgcg ctttcggaac gcacgtacga
      301 tcttgtgctc atggactgtc agatgcccgt gatggacggc tacgaggcag cccgccgcat
      361 ccgcgcactg gagaagcccg gcggaaagcg aataccggtt gtggcgatga ccgcctacgc
      421 gatgagcggc gaccgtgaac gctgcatcga ggcgggaatg gacgactatc tcgcaaagcc
      481 gatctcgatc gaggcgctgt cggccgccct tgcgtcttgg ttgccgcaca gggaaggacc
      541 cggcggaacg gacgggagcc cgcgccgatc cgagccgccg acgcctcggg aagaagcgga
      601 aacgtgctcg aaaagcgccg tctggagaaa ggacgaactg ctgaacagac ttctgggaga
      661 cgaggagatg acccgcgaaa ttctggaggc gtttcttgag gatattcccc gggagataga
      721 ttcgctcacc gccgccgtgg ccgtgggcaa cacacccacc gtggagctgc ttgctcacac
      781 catcaagggc gcggcgggga acgtgggaag cgaggggctg cgctcggcgg ccttcgaaat
      841 ggagtcggcc gccaagaagg gcgatctgtc cggagcgcag gcgctcctga agagcatacg
      901 acaggagttc gagcacctga aagcggcgat tcgaggataa acgcgcgccc cgctacgcca
      961 aaccggccgg ttcgtagacg ggagagggga gcctcggata ggcaaagtgg atgtggagat
     1021 agacttcgtc ggcgcgggag cggacgtcct cgttcgaata gcggttcacc ggcaggccgg
     1081 tcgcctcgct ccacagaaag
//
LOCUS       DAOTIL010001037         1099 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminobacteriaceae bacterium isolate AMR_MDS_3026
            NODE_160229_length_1099_cov_2.301724, whole genome shotgun
            sequence.
ACCESSION   DAOTIL010001037 DAOTIL010000000
VERSION     DAOTIL010001037.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868050
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminobacteriaceae bacterium (wastewater metagenome)
  ORGANISM  Aminobacteriaceae bacterium
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae.
REFERENCE   1  (bases 1 to 1099)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1099)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:19:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,224
            CDSs (total)                      :: 3,198
            Genes (coding)                    :: 3,184
            CDSs (with protein)               :: 3,184
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1099
                     /organism="Aminobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_160229_length_1099_cov_2.301724"
                     /isolate="AMR_MDS_3026"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:3034609"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1099)
                     /locus_tag="P5201_15485"
     CDS             complement(<1..>1099)
                     /locus_tag="P5201_15485"
                     /inference="COORDINATES: protein motif:HMM:NF015149.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006011 - UDP-glucose metabolic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="cellulose biosynthesis cyclic di-GMP-binding
                     regulatory protein BcsB"
                     /protein_id="HRW00002.1"
                     /translation="LRKEPLVRLPLPADLPRDRFLHARIESRIGITDDVCRDLFSGQL
                     FWIVQEDSGFSLDDLRISHRSVSDFFETLYGSLSILLPASPSREEAEAAVWLAAMLRK
                     AVPGLSVSLDTNENGRSASSSFILIAGDAIQLPSGIQPLPGLSLLGERGLLVLGDGKP
                     ASLAGFVKTIASFPLFAAIPSGSILPETSAASEKNAVSNLLNLAEGTGANAVSLEIPV
                     FPGLLASPPQDLSFQLEGACTLPRDPARPARLDVFWNGVFIESEYLSANGRFEKRLLL
                     PVETPLLTENRLRLDVSYHLDEGMCRYRGSENRATLLPSSSFHGYGKYPLHALSWNSF
                     GIFALRKGVLLLEEPFPIEGIRSAADMLHWLG"
BASE COUNT          265 a          310 c          350 g          174 t
ORIGIN      
        1 accgagccag tgaagcatat ccgcagcgct gcgaatgccc tcgatgggaa acggctcttc
       61 cagaagcaga acccctttgc ggagcgcaaa aataccgaag gagttccacg acagagcgtg
      121 cagagggtac ttcccgtagc cgtggaagga ggaggaagga agcagcgtcg cgcgattttc
      181 cgagccccgg tagcggcaca ttccctcatc gaggtgatac gatacgtcga gacgaagacg
      241 gttctccgtc aacagcggcg tttcgaccgg aaggagcagg cgtttttcga atcttccgtt
      301 tgcggacagg tactcgctct cgatgaacac gccgttccag aaaacgtcaa gacgcgccgg
      361 acgggcagga tcccgcggca gcgtacacgc cccttcgagc tggaaggaga gatcctgcgg
      421 cggcgaagcg aggagccccg ggaaaacggg gatttcaaga gagacggcgt tggcgcccgt
      481 tccctccgcc aggttcagca gatttgaaac ggcatttttt tcggaagccg cgctcgtctc
      541 gggcaaaata cttccggaag ggatcgccgc gaagagcggg aaagaagcga tcgtcttcac
      601 gaagccggcg agcgaagcag gcttgccgtc gccaagaaca aggagtcctc tctcgccgag
      661 cagcgataga cccggcaggg gctgaattcc ggaaggaagc tgaatggcgt ccccggcgat
      721 caggataaac gaagaggatg cggagcggcc gttctcgttc gtatccaaag aaacggaaag
      781 ccccgggacg gccttcctga gcatcgcggc aagccagacg gccgcttcgg cttcttctct
      841 tgaaggactg gccggcagca gaatgctgag cgaaccgtag agcgtctcga agaagtcgga
      901 gacggagcga tgcgatatcc tcaggtcgtc gagagagaac cccgaatcct cctgcacaat
      961 ccaaaagagc tgcccggaaa agagatcccg gcacacatcg tccgttatac ctatcctgga
     1021 ctcgatacgc gcgtgcagaa aacggtcgcg gggaaggtct gcgggtagcg gaagacggac
     1081 cagcggctcc ttgcgaagc
//
LOCUS       DAOTIL010001038         1098 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminobacteriaceae bacterium isolate AMR_MDS_3026
            NODE_160389_length_1098_cov_3.093960, whole genome shotgun
            sequence.
ACCESSION   DAOTIL010001038 DAOTIL010000000
VERSION     DAOTIL010001038.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868050
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminobacteriaceae bacterium (wastewater metagenome)
  ORGANISM  Aminobacteriaceae bacterium
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae.
REFERENCE   1  (bases 1 to 1098)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1098)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:19:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,224
            CDSs (total)                      :: 3,198
            Genes (coding)                    :: 3,184
            CDSs (with protein)               :: 3,184
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1098
                     /organism="Aminobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_160389_length_1098_cov_3.093960"
                     /isolate="AMR_MDS_3026"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:3034609"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..595)
                     /locus_tag="P5201_15490"
     CDS             complement(<1..595)
                     /locus_tag="P5201_15490"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRW00003.1"
                     /translation="MESSFSQYPLNRFIRTSGVSFLLSGTPGKSGAGKYVCDAFCSVC
                     QIREGDVDWGVFVGKGENFGVGEMHGFSVYDSTYGSLRLTKLIMPRLDEILKEAMRLA
                     EKNGASLVVETLAGMKEMLPLLEERSPQIYGADTNVACEDDGWLLMVAPGEKAILHDG
                     DSHIFEEVRVLSFVYSPKGMMYTLEPRRGESRWSVPRE"
BASE COUNT          271 a          315 c          257 g          255 t
ORIGIN      
        1 gttcccgagg cacactccag cgagattccc ctcttcgtgg ctccagagtg tacatcattc
       61 ctttgggtga atagacgaac gaaagcaccc ggacctcttc gaagatatgc gaatccccgt
      121 catgaagaat tgccttttca ccgggagcaa ccataagaag ccatccgtca tcttcacatg
      181 ctacattagt gtccgcaccg tatatttgcg gtgagcgttc ttcgagcaac ggaagcatct
      241 ccttcattcc tgcaagagtt tcaactacaa gagatgctcc attcttttcc gcaagacgca
      301 ttgcctcttt caatatctca tccaaacgag gcataatcaa ttttgtgagt cgcaacgacc
      361 cgtaggttga atcataaaca gagaacccgt gcatctctcc cacgccgaaa ttctctccct
      421 ttccgacgaa aactccccag tccacatctc cttcccggat ctggcagacg gagcagaatg
      481 cgtcgcagac atacttgccc gcgccgcttt tccccggtgt tcccgaaaga agaaagctga
      541 cgccgctcgt cctgataaag cggttcagag gatactgtga aaacgaactc tccattccgt
      601 aggtctcgtc ccgaaccaga ttccgtccaa tagtttcgcg gaacccaatc acacgttccg
      661 ccacttgcaa ttcgctttca caaacgtaaa agctctttga ccgcagcagt tgatgtattg
      721 cctgaaaaag aggaaacact actttttgaa tctttggcga tgttcgtgcg atagtcttgc
      781 tcttccgaac gacaatctct cccggaccat ggtatatccg attgacacga aatgcctgcg
      841 ccagatggcg atagaccgcg cctgggtatc cttcgcggag caattgggaa taggagagcg
      901 aaccgctcca gccgtacccg agcgtcagca catagttctt ttccgcgcac gtacgaagcg
      961 ggaaagcatg gtgcgggccg ttcgccgcct gctgtttcag agggtagaga tcctgaggaa
     1021 ccggatagac tgggtgcagc tcgttatgca gcagatggag aaagaccggg ttcaaatcgc
     1081 tgaacggcgc ttccgaac
//
LOCUS       DAOTIL010001039         1098 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminobacteriaceae bacterium isolate AMR_MDS_3026
            NODE_160424_length_1098_cov_2.673058, whole genome shotgun
            sequence.
ACCESSION   DAOTIL010001039 DAOTIL010000000
VERSION     DAOTIL010001039.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868050
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminobacteriaceae bacterium (wastewater metagenome)
  ORGANISM  Aminobacteriaceae bacterium
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae.
REFERENCE   1  (bases 1 to 1098)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1098)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:19:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,224
            CDSs (total)                      :: 3,198
            Genes (coding)                    :: 3,184
            CDSs (with protein)               :: 3,184
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1098
                     /organism="Aminobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_160424_length_1098_cov_2.673058"
                     /isolate="AMR_MDS_3026"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:3034609"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..774)
                     /locus_tag="P5201_15495"
     CDS             complement(<1..774)
                     /locus_tag="P5201_15495"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRW00004.1"
                     /translation="MNILFCSDAMIVDGVTSFVFHLATALSEGGHRVAVLGRWGGKGF
                     QSRLRTHGVEVISIPSVTVGNFWFDKKAREFAPDVLVTDSRRSFPLAVRLKRATGAKI
                     FTFFLDNLEKTDKKGRDVDSLVRWSDAWLSAEPPLLEKLEEIPTPFPKFLFQRPLTGL
                     LTPTPIPSRDPFRVLCFGRISGYKSAGPLKLLQEAPMLKKSIPSLEIAFVGGGWRTWK
                     FRLMAARANAAAGERYLRIIGTQTDPQRWFEWATLVCAGS"
     gene            complement(820..>1098)
                     /locus_tag="P5201_15500"
     CDS             complement(820..>1098)
                     /locus_tag="P5201_15500"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRW00005.1"
                     /translation="GKLRVAMQDLTASLVRRRLLPPGVLWGKPRFDALFDVLERRGFL
                     KEMQPRALRRSSSGGASLHADGPVLNEARRAAEWIVERWGETGKDNRK"
BASE COUNT          217 a          356 c          331 g          194 t
ORIGIN      
        1 ggacccggcg cagacgagcg tcgcccattc gaaccagcgc tggggatcgg tctgcgtccc
       61 gatgatccga aggtaccttt cccccgccgc agcgttggcg cgtgcggcca tcaggcgaaa
      121 cttccacgtc ctccaccctc cgccgacgaa ggcgatctcg agcgacggga tgctcttttt
      181 cagcatcggc gcctcctgca acagcttcaa gggcccggcc gacttgtacc cgcttattct
      241 gccgaaacag aggacccgga acgggtcgcg cgaaggaatc ggcgtcgggg taagcagacc
      301 ggtgagaggg cgctggaaga ggaacttcgg gaacggggtc ggtatctcct ccagcttctc
      361 caaaagcgga ggctctgcag agagccatgc gtcgctccag cgcaccaggg aatcgacgtc
      421 gcgccccttc ttgtccgttt tttccaggtt gtccagaaag aaggtgaaaa ttttcgcccc
      481 cgtcgctcgt ttgagccgca cggcaagagg gaaggagcgt cgggaatccg tgaccagaac
      541 atctggcgca aactcacgag ccttcttatc gaaccagaag tttccgactg tcacggaggg
      601 aatggagatc acctccacgc cgtgcgtgcg gaggcgggat tgaaacccct tgccgcccca
      661 ccgtccgaga acggcgaccc gatgccctcc ttcgctgagg gcagtcgcaa gatgaaaaac
      721 aaacgaagtg acgccgtcga cgatcatggc gtccgaacaa aaaagtatgt tcatccgaga
      781 atggagctcc ttttatcggc gcgtacacac gccgcgcgtt tattttctgt tgtcctttcc
      841 cgtttctccc caccgttcga cgatccactc cgccgcccgc cgcgcctcgt tgaggacggg
      901 gccgtcggcg tggagcgacg cgcctcccga ggaagagcgt cgaagggcgc ggggctgcat
      961 ctccttcaga aatccacggc gttccagtac gtcgaagagg gcgtcgaagc gcggtttgcc
     1021 ccaaagcacc cccggcggca gcagccgcct ccgtacgagc gacgccgtga gatcctgcat
     1081 cgcgacgcgg agcttccc
//
LOCUS       DAOTIL010001040         1098 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminobacteriaceae bacterium isolate AMR_MDS_3026
            NODE_160425_length_1098_cov_2.672100, whole genome shotgun
            sequence.
ACCESSION   DAOTIL010001040 DAOTIL010000000
VERSION     DAOTIL010001040.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868050
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminobacteriaceae bacterium (wastewater metagenome)
  ORGANISM  Aminobacteriaceae bacterium
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae.
REFERENCE   1  (bases 1 to 1098)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1098)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:19:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,224
            CDSs (total)                      :: 3,198
            Genes (coding)                    :: 3,184
            CDSs (with protein)               :: 3,184
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1098
                     /organism="Aminobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_160425_length_1098_cov_2.672100"
                     /isolate="AMR_MDS_3026"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:3034609"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..986)
                     /locus_tag="P5201_15505"
     CDS             complement(<1..986)
                     /locus_tag="P5201_15505"
                     /inference="COORDINATES: protein motif:HMM:NF018513.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0031317 - tripartite ATP-independent
                     periplasmic transporter complex [Evidence IEA];
                     GO_function: GO:0015556 - C4-dicarboxylate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015740 - C4-dicarboxylate transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter large permease subunit"
                     /protein_id="HRW00006.1"
                     /translation="MLLMGSIFLVLLLLGMPIAFTIGISAMAFVFTEEFIPLTIAVQK
                     MISSTQSFPLLAVPFFVLAGNLMNATGITSRLITFAHVLTGHLIGGLAHVSVVLSALM
                     GGISGSAVADAAMESRLLGPSMIKAGYSKGYAAAVIALSSLITATIPPSIGLILYGFV
                     GEVSIGKLFIGGIVPGLLMTFILMGVSWLTAKNRGYGRARETRATLPEFKEAFKESFL
                     ALLFPLILIVGIRFGIFTPSEAGAFAAVYAFVVGKFVYKELTWETLKEVLRQTVRDNG
                     IIMLIITCSGIFGYAIVYNRMPQTIAQWLLGVTNSPGLLLFIVLTFLFIAGM"
BASE COUNT          231 a          325 c          373 g          169 t
ORIGIN      
        1 aacatgcccg cgatgaagag gaacgtgagg acgatgaaga gcagcagtcc cgggctgttc
       61 gtcacgccca gcagccactg ggcgatcgtc tgcggcatgc ggttgtagac gatcgcatag
      121 ccgaagatcc ccgaacaggt gatgatgagc atgatgatgc cgttgtcgcg gaccgtctgc
      181 cggagcactt ccttcagcgt ctcccacgtc agctccttgt agacgaactt ccccacgacg
      241 aaggcgtaga ccgcggcgaa cgcgcccgcc tcggacggcg tgaagatgcc gaagcggatg
      301 ccgacgatca ggatgagcgg gaagagaagc gccagaaagc tctccttgaa ggcttcctta
      361 aactcgggga gcgtggcgcg cgtctcgcgg gcccgtccgt agccgcggtt tttcgccgtc
      421 agccatgaga cgcccatcag gatgaaggtc atcaggagtc ccggaacgat gccgccgatg
      481 aagagcttgc cgatggagac ctccccgacg aaaccgtaga ggataaggcc gatgctcggc
      541 ggaatggtgg cggtgatcag cgaggagagg gcgatgacgg cggccgcgta ccccttcgag
      601 taccctgcct tgatcatgga agggccgagg aggcgcgact ccatagcggc gtccgccact
      661 gcggagccgg agatgccgcc catcagcgcg ctgaggacga cgctgacgtg ggcgagcccg
      721 ccgatgaggt gccccgtgag cacgtgcgcg aacgtgatga gcctgctcgt gatgcccgtg
      781 gcgttcatca gattgcccgc gaggacgaag aaggggacgg cgagcagcgg aaaggactgc
      841 gtcgagctga tcatcttctg gacggcgatg gtcagcggga tgaactcctc ggtgaagacg
      901 aaagccatcg cggagatgcc gatggtgaag gcgatgggca tacccagcag caacagaaca
      961 aggaagatgc ttcccatcag cagcatggat tcgtccctcc ttcggatccc gcctcgttcg
     1021 cgcgctcccg caggagctgc tcgaccgtgg tgcggagcat cagcagacag ccgacgggaa
     1081 cgctcagcgt cgcccacg
//
LOCUS       DAOTIL010001041         1096 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminobacteriaceae bacterium isolate AMR_MDS_3026
            NODE_160831_length_1096_cov_3.574448, whole genome shotgun
            sequence.
ACCESSION   DAOTIL010001041 DAOTIL010000000
VERSION     DAOTIL010001041.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868050
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminobacteriaceae bacterium (wastewater metagenome)
  ORGANISM  Aminobacteriaceae bacterium
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae.
REFERENCE   1  (bases 1 to 1096)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1096)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:19:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,224
            CDSs (total)                      :: 3,198
            Genes (coding)                    :: 3,184
            CDSs (with protein)               :: 3,184
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1096
                     /organism="Aminobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_160831_length_1096_cov_3.574448"
                     /isolate="AMR_MDS_3026"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:3034609"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>1096
                     /gene="murJ"
                     /locus_tag="P5201_15510"
     CDS             <1..>1096
                     /gene="murJ"
                     /locus_tag="P5201_15510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005661611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0034204 - lipid translocation [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="murein biosynthesis integral membrane protein
                     MurJ"
                     /protein_id="HRW00007.1"
                     /translation="RVLAAKGKESAERLARQAFSVLLVATILSVAAGVVFSPLLVKIM
                     APGFDPVKAELATAMTRWMFPFLVLVSLAALAMGVLNSMGSFLVPALAPALSNLVYIV
                     LVVFLASFYGVWGLVIAVLAGGACQFLLQWAWSVRMGVTLLPEWPQLKDPDLRTMLAL
                     FLPYAAGLSLNQVNPVISRMLASFLQEGAISVLNYANRVIQLPLGIFVIAISQAVLPQ
                     LSRCPAEDTGEFRDIMRDSLRFTLFVVFPATLGLALVSDEIVHLLFVRGAFGEWAWKG
                     TSVSLAMYSLGLPGMAFSTVVMRGLYARSLPRGALIVTLSSVAATLLFSLALMGPLSF
                     GGLALATSLAFSLSGWVGVRILSRHLGMRFG"
BASE COUNT          137 a          352 c          340 g          267 t
ORIGIN      
        1 caagggttct ggcggccaag ggaaaagaaa gcgcggagcg cctcgcgcgt caggcctttt
       61 ccgtgctgct cgtcgctacg attctctccg tcgcggcggg cgttgtgttt tccccgctgc
      121 tcgtaaagat tatggctccg gggtttgatc ctgtcaaagc cgaattggcg acggcgatga
      181 cgcgctggat gtttcccttc cttgtcctgg tgtcgcttgc ggcgctcgcg atgggggtgc
      241 tcaacagcat gggctccttt ctcgttcccg cgcttgcccc ggcgttgagc aatctcgtct
      301 acatcgtgct ggttgtgttc cttgcctctt tctacggtgt ttggggcctc gtgatcgcgg
      361 tgctcgccgg tggcgcctgc cagtttcttc tccaatgggc gtggtcggta cggatggggg
      421 ttacgctgct ccccgagtgg ccgcaactga aggatccaga tctccggacg atgctcgccc
      481 tcttccttcc gtacgccgcc ggactctcgc tgaaccaggt gaatccggtg atcagcagga
      541 tgctcgcctc ctttctccag gagggcgcca tctctgtgct gaactacgca aaccgcgtga
      601 tccagctccc cctgggaatt ttcgtcatcg cgatatcgca ggccgtcctg ccccaactct
      661 cccgatgccc ggcggaagat accggggagt tccgcgacat catgcgcgac tcgctcaggt
      721 tcacgctctt cgtcgtcttt cccgcgacgc tgggcctggc gcttgtctcg gacgaaatcg
      781 tccatctgct cttcgtcagg ggagcgttcg gcgagtgggc ctggaaaggg acctcggttt
      841 cactcgcgat gtactctctc gggcttcccg gaatggcgtt ctccacggtg gtcatgcggg
      901 ggctctacgc ccgaagcctg ccgcgcgggg cgcttatcgt cactctctcc agcgtggcgg
      961 ccacgctgct cttttcgctt gcgctgatgg ggccgctctc gttcggaggc ctcgcgctcg
     1021 caacgtcgct ggcgttcagc ctctccgggt gggtgggcgt ccgcatactt tcccgccact
     1081 tagggatgcg gttcgg
//
LOCUS       DAOTIL010001042         1094 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminobacteriaceae bacterium isolate AMR_MDS_3026
            NODE_161268_length_1094_cov_3.181906, whole genome shotgun
            sequence.
ACCESSION   DAOTIL010001042 DAOTIL010000000
VERSION     DAOTIL010001042.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868050
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminobacteriaceae bacterium (wastewater metagenome)
  ORGANISM  Aminobacteriaceae bacterium
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae.
REFERENCE   1  (bases 1 to 1094)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1094)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:19:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,224
            CDSs (total)                      :: 3,198
            Genes (coding)                    :: 3,184
            CDSs (with protein)               :: 3,184
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1094
                     /organism="Aminobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_161268_length_1094_cov_3.181906"
                     /isolate="AMR_MDS_3026"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:3034609"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>1094
                     /locus_tag="P5201_15515"
     CDS             <1..>1094
                     /locus_tag="P5201_15515"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HRW00008.1"
                     /translation="GPSRGLLIVGALLQKRLPLMRESLDTQFDILPLSEDAAPERSSE
                     DIRIAPNESGGASVWQARGSAVIRIDSPRTIYEKGRDAVMASYLWILLAGMGILIVVT
                     LLLETFVLARLKRLQTLSDKIAAEGMIEARVPLEGKDEITALSRSYNTLLHTLENLLM
                     DIPDAFFLVDPEGVIVMSNEAARAALKQDDGADLRGRDIAAFLGREKLDFYRKFDEPA
                     EVGAGEGSVFEASLLRLDGALVPVEVHRRRIEYGDRTLLLLLARDLTERKQFEERLAQ
                     KAYFDAMTGLPNRHSFVRALNRALNAGDALAVAVMNMDHFKLVNAQVGNLNGDRVLMM
                     TAERIRTLLEPPAQLYRTGGDEFAALIPLA"
BASE COUNT          205 a          325 c          356 g          208 t
ORIGIN      
        1 cggtccctcc cgcgggctgc tgatcgtggg cgctctgttg caaaaacgtc ttcctctcat
       61 gcgggagagc cttgacacgc agttcgacat ccttcccctg tcagaggacg cagcgcccga
      121 gagatcttcg gaagatatcc ggatcgcgcc gaacgaaagc ggcggggcgt cggtctggca
      181 ggcgagaggg agcgccgtaa tccgtatcga ttccccgcgg accatttacg aaaagggaag
      241 agacgcggta atggcttcct acctgtggat tctgctcgcc ggtatgggca tactcattgt
      301 ggtgacgctg ctgcttgaga cctttgtcct tgcgcggctg aagcggcttc agacattgtc
      361 cgacaaaatc gccgccgaag ggatgatcga agcccgcgtt cccttggagg ggaaggacga
      421 gatcaccgct ctttcccgct cgtacaatac cctgctgcac accttggaaa acctgctgat
      481 ggatatcccc gacgcgttct tcctggtcga cccggagggt gttatcgtca tgtccaacga
      541 agcggcgcgc gcggcgctga agcaggacga cggcgcggat ctgaggggcc gggacatcgc
      601 cgcattcctc ggaagagaga agctcgattt ctacaggaag ttcgacgagc ccgcggaggt
      661 cggcgcggga gaaggttccg tcttcgaggc gtcgcttctc cgtttggacg gcgcgctcgt
      721 tcccgtggag gtgcaccggc ggcgtatcga gtacggcgac cggacgcttt tgctgctcct
      781 cgcgcgcgac ctcaccgagc ggaagcagtt cgaggaacgc ctggcccaga aagcctattt
      841 cgacgccatg acggggcttc cgaaccgaca ctccttcgtc agggcgctga accgtgcgtt
      901 gaacgcgggg gatgcgcttg ccgtcgcggt gatgaacatg gaccacttca aactggtgaa
      961 cgcgcaggtt ggaaatttga acggcgacag ggtgctcatg atgaccgccg agagaatccg
     1021 cacgcttctg gagcccccgg cgcagctcta cagaacgggg ggcgacgagt tcgccgcgct
     1081 gatcccgctt gcgg
//
LOCUS       DAOTIL010001043         1093 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminobacteriaceae bacterium isolate AMR_MDS_3026
            NODE_161558_length_1093_cov_2.539499, whole genome shotgun
            sequence.
ACCESSION   DAOTIL010001043 DAOTIL010000000
VERSION     DAOTIL010001043.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868050
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminobacteriaceae bacterium (wastewater metagenome)
  ORGANISM  Aminobacteriaceae bacterium
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae.
REFERENCE   1  (bases 1 to 1093)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1093)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:19:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,224
            CDSs (total)                      :: 3,198
            Genes (coding)                    :: 3,184
            CDSs (with protein)               :: 3,184
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1093
                     /organism="Aminobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_161558_length_1093_cov_2.539499"
                     /isolate="AMR_MDS_3026"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:3034609"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..666)
                     /locus_tag="P5201_15520"
     CDS             complement(<1..666)
                     /locus_tag="P5201_15520"
                     /inference="COORDINATES: protein motif:HMM:NF033516.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HRW00009.1"
                     /translation="MSTLSLHERRALVDWDEKEIPLSWQAWLLGLNRSGLYYVPAPLS
                     EEELLLRRRIDEINTLHPCYGSRRMRAVLSRGGLSVSRKRIQRLMRDMGILSIYPAPK
                     LSAGDESPKFPYLLRGVDIDHPNHTWGTDITYVRLRGGFMYLTVFLDWYSRYVVSWAF
                     SDSLSAGFVIEALEDAFKTARPKIINSDQGSQYTSKSYVERIRDESDGIAISMDGRGR
                     CMDN"
BASE COUNT          220 a          332 c          287 g          254 t
ORIGIN      
        1 gttgtccatg caacgccccc ttccgtccat gctgatggcg atgccgtcgc tttcgtcccg
       61 aatccgctcg acatacgatt tgctcgtgta ctgcgacccc tgatcgctgt tgatgatttt
      121 cgggcgggcc gtcttgaagg cgtcctccag cgcctcgatc acgaaccccg ccgacagcga
      181 gtccgaaaat gcccacgaga ccacgtagcg ggagtaccag tccagaaaca ccgtaagata
      241 catgaagccg cccctcagcc ggacgtaggt gatgtccgtc ccccacgtgt ggttcggatg
      301 atcgatgtcc actccgcgca gaagataggg gaacttcgga ctctcgtcgc ccgcgctcag
      361 tttgggagcg gggtatatcg agaggattcc catgtctctc atgagacgct gaatccgttt
      421 gcgcgatacg gacaatcccc cccgcgacag caccgcccgc atcctcctgg agccgtagca
      481 cggatgcaac gtgttgatct cgtcgatcct cctgcggagc agcagttctt cctccgagag
      541 aggcgcgggg acgtagtaca gcccgcttct gttcagaccg agtagccagg cctgccagga
      601 gagagggatc tccttttcat cccagtccac gagcgctctg cgttcatgaa gagaaagagt
      661 cgacaccgca tttttttttg acccattcga gctgcatgga aagcaacccg acttgtctgt
      721 aaaggtcgtt tatcgtagaa tccttcccga gatcctcggc gcttttcctg cggggatcgg
      781 agaacaatcc cggaagccct tccgtggccg tctttttcca tctctgaagc aggttgggat
      841 gtattttgtg cttggctgcg atctggctga gagtttcttc ttcacgaagc atctcgagca
      901 caacttttgc cttgaattca gcagaaaagt tccgacgcga actcatcttt tacaccttaa
      961 cctccccccg actttttgtc cgattttcgg ggtccattat aacctgcgcg gagcttaaga
     1021 tccaccggcg cgctctgaga gagccggggt tacgtacttc aaggtgcaaa acgcgcccaa
     1081 attgagccac ccg
//
LOCUS       DAOTIL010001044         1091 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminobacteriaceae bacterium isolate AMR_MDS_3026
            NODE_162067_length_1091_cov_2.267375, whole genome shotgun
            sequence.
ACCESSION   DAOTIL010001044 DAOTIL010000000
VERSION     DAOTIL010001044.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868050
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminobacteriaceae bacterium (wastewater metagenome)
  ORGANISM  Aminobacteriaceae bacterium
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae.
REFERENCE   1  (bases 1 to 1091)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1091)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:19:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,224
            CDSs (total)                      :: 3,198
            Genes (coding)                    :: 3,184
            CDSs (with protein)               :: 3,184
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1091
                     /organism="Aminobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_162067_length_1091_cov_2.267375"
                     /isolate="AMR_MDS_3026"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:3034609"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1091)
                     /locus_tag="P5201_15525"
     CDS             complement(<1..>1091)
                     /locus_tag="P5201_15525"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRW00010.1"
                     /translation="QGTAEERIPLRFGKNDIEVRLLDAKRAERVLSVDVTSTGQRAAE
                     EDTPEKDKKDAPEIPPEAPEAHEEQEPGDETPPQMPEIPGGDEEVDSLLDQIQELMGM
                     PPEGGTPPDHEGPEGAEDGPLPGGDDQEPETAQDPGTINIPDVGTVDVTGPSDFLLPA
                     EEDEDDDSWLDAGGDLEEEEPLEPEEIEIEEEGGEDVGDEPDDEEITGEEFFTETDLF
                     DGFDGLDEETEPLDLEAWVEPKGNPDAPEQDPMYECPLEPFDAADTDPSSPFTPPSGL
                     QSDPGGCVAVETVQKDWYCTNRPNINVKFQLPDWLKKLDLPKPGTKEYDEMVKKLLQH
                     LRDRGIDTAAFERFQDALIRRAGRLEHPD"
BASE COUNT          135 a          381 c          321 g          254 t
ORIGIN      
        1 gtcggggtgc tccagcctcc ccgccctccg gatcagggcg tcctggaaac gttcaaacgc
       61 cgcggtgtcg atgcccctgt cgcgcagatg ctgcagaagc ttcttcacca tttcgtcgta
      121 ctctttcgtc cccggcttgg gcaggtcgag cttcttcagc cagtcgggga gctggaactt
      181 cacattgatg ttcggcctgt tggtgcagta ccagtctttc tggacggttt ccacggccac
      241 gcagccgccc ggatcgcttt gcagccccga cggtggcgtg aagggcgagc tcgggtcggt
      301 gtcggcggcg tcgaacggtt ccagggggca ctcgtacatc gggtcctgct cgggagcgtc
      361 cggattgccc ttcggctcca cccacgcttc gagatccaga ggctccgttt cttcgtccag
      421 cccgtcgaat ccgtcgaaga ggtcggtttc ggtgaagaac tcctctccgg taatctcctc
      481 gtcgtcgggc tcgtcgccga cgtcctcgcc gccctcttcc tcgatctcga tctcctcggg
      541 ttccagcggc tcttcctctt cgagatcgcc gcccgcgtcg agccacgagt cgtcgtcctc
      601 gtcctcctcg gcaggcagca ggaagtcgga gggccccgtc acatcgacgg ttcccacatc
      661 gggaatgttg atcgtcccgg gatcctgcgc cgtctccggc tcctgatcat cccctccggg
      721 cagaggaccg tcttccgcac cctccggccc ctcatgatcc ggaggcgtcc cgccctcggg
      781 gggcattccc atgagctctt gtatctggtc gagaagggag tcgacctctt cgtccccgcc
      841 gggaatctcc ggcatttggg gaggcgtttc atctcctggc tcttgctcct catgcgcttc
      901 aggcgcttcc ggaggtatct ccggcgcatc ctttttgtcc ttttctgggg tgtcttcctc
      961 cgctgcgcgc tgcccggtgg aggtcacgtc gacggagagc accctttcgg ctcgcttcgc
     1021 gtcgagcagt ctgacctcga tgtcgttttt cccgaagcgc agcggaatac gctcctccgc
     1081 cgtcccctgc g
//
LOCUS       DAOTIL010001045         1090 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminobacteriaceae bacterium isolate AMR_MDS_3026
            NODE_162305_length_1090_cov_2.260870, whole genome shotgun
            sequence.
ACCESSION   DAOTIL010001045 DAOTIL010000000
VERSION     DAOTIL010001045.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868050
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminobacteriaceae bacterium (wastewater metagenome)
  ORGANISM  Aminobacteriaceae bacterium
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae.
REFERENCE   1  (bases 1 to 1090)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1090)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:19:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,224
            CDSs (total)                      :: 3,198
            Genes (coding)                    :: 3,184
            CDSs (with protein)               :: 3,184
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1090
                     /organism="Aminobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_162305_length_1090_cov_2.260870"
                     /isolate="AMR_MDS_3026"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:3034609"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1044)
                     /gene="asnB"
                     /locus_tag="P5201_15530"
     CDS             complement(<1..1044)
                     /gene="asnB"
                     /locus_tag="P5201_15530"
                     /EC_number="6.3.5.4"
                     /inference="COORDINATES: protein motif:HMM:TIGR01536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004066 - asparagine synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006529 - asparagine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine synthase (glutamine-hydrolyzing)"
                     /protein_id="HRW00011.1"
                     /translation="MRSMNTLQKHRGPDGSGEWVRDDESVAFGHLRLSIIDLSPEAAQ
                     PMTDGHGNWITYNGEIYNYRELRKELGEDNFRTSSDTEVILRAYERWGESCVQRFRGM
                     FSFALWDETQQTLFCARDRFGIKPFYYAVVDGVLFFASEVKALLPFLPDIETDVDGLK
                     DYLTFQFCLDGKTLFEGVRELPPAHFLKVKNGFTSIERYWEVYYTLDFDHTEKFFQER
                     LASLLSEAVSLHMRSDVPVGAYVSGGLDSSTVAALATYLVPGNEMMGFNGRFSQFGEA
                     FDESMYAEALSLHGGFDLKVCDITCADFHENIRSVVYHMDFPVAGPGVLPQYLVSQLA
                     SKHRKVVLGGQGGD"
BASE COUNT          271 a          312 c          282 g          225 t
ORIGIN      
        1 gtcccccccc tgaccgccaa gaacaacctt cctgtgcttt gaggccaact gcgagacaag
       61 gtactgaggc aacacacccg gccctgcgac cgggaaatcc atatgataaa caacactcct
      121 aatattctca tggaaatcgg cacaagtaat gtcgcacacc ttaagatcga acccgccgtg
      181 cagagagagt gcctcggcgt acatgctctc gtcaaatgcc tcgccgaact ggctgaatct
      241 gccattgaaa cccatcatct cgtttcccgg aactaggtaa gtcgcaagag cggccacggt
      301 actcgaatcc agcccacctg aaacgtaggc gcccacaggg acgtcgctgc gcatgtggag
      361 cgaaacagcc tccgagagca gcgaggcgag gcgctcctgg aagaatttct cggtatggtc
      421 aaaatcaagg gtgtagtaga cttcccagta ccgctcgatg cttgtgaaac cgtttttcac
      481 tttcaagaaa tgcgccgggg gtagctcgcg cactccttcg aaaagggtct ttccgtccag
      541 gcaaaactga aaggtcaggt agtctttcag cccgtcgacg tcggtctcga tatccggcaa
      601 aaagggaaga agcgccttta cctcggacgc gaagaagagc acaccgtcca caactgcgta
      661 gtagaaaggc ttgatgccga atcggtcccg tgcgcagaaa agtgtttgtt gtgtttcgtc
      721 ccacagggca aatgaaaaca taccccggaa acgctggaca cacgactccc cccaccgctc
      781 gtaagcacgg agaataactt ccgtatccga tgatgtgcga aaattatcct ccccgagttc
      841 cttgcgaagt tcgcggtagt tgtaaatctc gccgttatag gtgatccagt ttccgtgtcc
      901 gtcggtcatc ggttgcgccg cctctggaga gaggtctata atgctcagac gcagatgacc
      961 gaaggcgact gattcatcgt cgcgcaccca ctccccggaa ccgtccggac cccgatgttt
     1021 ctggagggtg ttcatagacc ttatggagcc tcgacagttc aggacagggg ctcctgagat
     1081 gttaaaaata
//
LOCUS       DAOTIL010001046         1090 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminobacteriaceae bacterium isolate AMR_MDS_3026
            NODE_162325_length_1090_cov_2.144928, whole genome shotgun
            sequence.
ACCESSION   DAOTIL010001046 DAOTIL010000000
VERSION     DAOTIL010001046.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868050
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminobacteriaceae bacterium (wastewater metagenome)
  ORGANISM  Aminobacteriaceae bacterium
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae.
REFERENCE   1  (bases 1 to 1090)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1090)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:19:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,224
            CDSs (total)                      :: 3,198
            Genes (coding)                    :: 3,184
            CDSs (with protein)               :: 3,184
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1090
                     /organism="Aminobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_162325_length_1090_cov_2.144928"
                     /isolate="AMR_MDS_3026"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:3034609"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1090)
                     /locus_tag="P5201_15535"
     CDS             complement(<1..>1090)
                     /locus_tag="P5201_15535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006583105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009306 - protein secretion [Evidence IEA];
                     GO_process: GO:0044780 - bacterial-type flagellum assembly
                     [Evidence IEA];
                     GO_process: GO:0071978 - bacterial-type
                     flagellum-dependent swarming motility [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="flagellar biosynthesis protein FlhA"
                     /protein_id="HRW00012.1"
                     /translation="LNAGLIDDAGAKDRRLTIQREADFYGAMDGASKFVKGDAIAGLI
                     ITIINIVGGLAIGVLQRGFPLEKALATYSLLTVGDGLVSQIPALLFSTATGVIVTRAA
                     GQTDLGKDIFTSLTTYPRPLFIGSILLFALAAVPGLPKIPFLLLAAFLCLMGYWVYRE
                     GKIQEEAGGKKREGRKEAAPGAPGGEAPKPAPSSPEDVMRLLTVDPMEAEIGYALIPL
                     VDPSQGGDMLDRIGTIRKQMAMELGIVVPPIRIRDNIQIKPTEYILRVKGAETGRGEL
                     LPDHYLAMNTGGAEEELVGVPTTEPSFGLPAVWIPPEMRDKAESLGYTVVDAPSVLAT
                     HLSETIKRYGADLLTRQEVQKLTDMVKEA"
BASE COUNT          220 a          339 c          321 g          210 t
ORIGIN      
        1 agcctccttc accatatcgg tgagtttctg cacctcctgc cgcgtcagca gatcggcgcc
       61 gtagcgttta atcgtctccg aaaggtgggt cgccaatact gaaggcgcat cgacgacggt
      121 gtaccccagc gactcggcct tatcgcgcat ctcgggagga atccagaccg caggaagccc
      181 gaaagagggt tcggtggtgg gaacgccgac aagctcctcc tccgcgcctc cggtgttcat
      241 ggcaagatag tgatcgggca gcagctctcc gcgcccagtt tcggccccct tgacgcggag
      301 aatgtactcc gtcggcttga tctggatgtt atcgcgaatc cgtatcggcg gtacgacgat
      361 ccccagctcc atcgccatct gtttgcggat tgttcctatc cggtcaagca tgtccccgcc
      421 ctgcgaagga tccaccagcg ggatgagggc ataccctatc tccgcctcca tcggatcgac
      481 cgtcagcaga cgcatcacat cttcaggaga ggagggagcc ggtttcgggg cctcgccccc
      541 gggagcgccc ggagcggctt cctttcgacc ttcccttttc ttgccgcccg cttcctcctg
      601 tatctttccc tcgcggtaga cccagtaccc catcaggcag agaaaggctg ccaaaagcag
      661 aaagggaatc tttggaagac cggggaccgc agcaagtgcg aaaaggagga tggaacctat
      721 gaaaagaggt ctgggatagg tcgtgagcga cgtaaagata tccttgccga ggtccgtctg
      781 ccctgccgca cgggtgacga tgacgccggt agccgtcgag aagagcagcg ccgggatctg
      841 cgaaacaaga ccgtcgccca ccgtcagaag gctgtaggtc gcaagcgcct tctccagagg
      901 gaacccccgc tgcaaaacac cgatggcaag acctccaacg atgttgatga tcgtgatgat
      961 caggcctgcg atggcgtcgc ccttaacgaa cttggatgcg ccgtccatcg cgccgtaaaa
     1021 gtccgcttcg cgttgaatgg tgaggcgccg gtccttggct cccgcgtcgt cgatcagacc
     1081 ggcgttcaag
//
LOCUS       DAOTIL010001047         1089 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminobacteriaceae bacterium isolate AMR_MDS_3026
            NODE_162519_length_1089_cov_2.650870, whole genome shotgun
            sequence.
ACCESSION   DAOTIL010001047 DAOTIL010000000
VERSION     DAOTIL010001047.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868050
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminobacteriaceae bacterium (wastewater metagenome)
  ORGANISM  Aminobacteriaceae bacterium
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae.
REFERENCE   1  (bases 1 to 1089)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1089)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:19:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,224
            CDSs (total)                      :: 3,198
            Genes (coding)                    :: 3,184
            CDSs (with protein)               :: 3,184
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1089
                     /organism="Aminobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_162519_length_1089_cov_2.650870"
                     /isolate="AMR_MDS_3026"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:3034609"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(36..>1089)
                     /locus_tag="P5201_15540"
     CDS             complement(36..>1089)
                     /locus_tag="P5201_15540"
                     /inference="COORDINATES: protein motif:HMM:NF012307.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="reverse transcriptase domain-containing protein"
                     /protein_id="HRW00013.1"
                     /translation="RWIIEGDLTSYFDTIHHRLLMKAVRKRIRDRRFLDLLWKFLKAG
                     HVDKRLFHAAHDGVPQGGVISPLLGNIMLNEFDMYLENRYLSKKTRRARESWNDSVKR
                     KRPKATKEGRKLKPSVSYCRYADDFVISVKGTRAQTEEIREECKEYLHNDLRLVLNME
                     KTRITHVNDGFVFLGHRIIRKRGKKGTMRVTDGIPREKVSGFTDRIRKHLDENHHMSG
                     AEMIDSLNKKIDGWGRFYQYADQKTWAYKRVDNTVFWKMARWLGAKYRSAVKPLIRKW
                     VAHPEEGKAKTWVMYDRTSDGKPRRAVLSRLVGRGKMQYRWKQQQGNPYRVEPEERTQ
                     APASRYGEVAMAVSQR"
BASE COUNT          163 a          331 c          279 g          316 t
ORIGIN      
        1 cgtacgtgcg cctctcaacg catacggctc tccacttaac gttgactgac agccatcgcg
       61 acttcgccgt aacgagacgc cggtgcttgg gttcgttcct ccggctccac cctgtaggga
      121 ttgccttgct gctgtttcca tcggtactgc attttccctc ttcctacaag ccgagagaga
      181 actgcgcgtc ttggcttgcc gtcgctcgtc ctgtcgtaca tcacccatgt tttggccttc
      241 ccctcttcgg ggtgcgctac ccacttcctt atcaggggct ttacggcact tctatacttc
      301 gcccccagcc agcgcgccat cttccagaag accgtgttgt ccaccctttt ataggcccat
      361 gtcttctggt ccgcgtactg gtagaatctc ccccagccgt cgatcttctt gttcaggctg
      421 tcgatcatct ccgcgccgct catgtggtga ttttcgtcga gatgcttcct gatgcggtct
      481 gtgaagccgg agactttctc gcgggggatt ccgtcagtga ctctcatcgt tcccttcttt
      541 ccccgctttc tgatgatgcg gtgtccgaga aagacgaatc cgtcgttgac gtgggtgata
      601 cgggtctttt ccatgttcag gacgagtcgg aggtcgttgt gcaggtattc cttgcactct
      661 tcgcgtatct cctccgtctg tgctcgcgtt cctttgacgg atatcacgaa gtcgtcggcg
      721 tatcggcagt agctcaccga aggtttcagt ttgcgtcctt ccttggttgc tttcggacgt
      781 ttccgcttga cgctgtcgtt ccagctttct ctggctcttc gcgtcttctt ggagaggtac
      841 cggttttcaa ggtacatgtc gaattcgttc agcatgatgt ttccgagcag cggtgagatg
      901 actccgccct gaggcacgcc gtcgtgcgcg gcgtgaaaga gccgcttgtc gacgtgcccc
      961 gccttgagga acttccagag caggtccaga aacctcctgt cccggattct cttcctcacg
     1021 gccttcatta gcaatctgtg atgtatggtg tcgaagtagc tcgtcagatc cccttcgatt
     1081 atccatcgg
//
LOCUS       DAOTIL010001048         1088 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminobacteriaceae bacterium isolate AMR_MDS_3026
            NODE_162697_length_1088_cov_3.706680, whole genome shotgun
            sequence.
ACCESSION   DAOTIL010001048 DAOTIL010000000
VERSION     DAOTIL010001048.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868050
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminobacteriaceae bacterium (wastewater metagenome)
  ORGANISM  Aminobacteriaceae bacterium
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae.
REFERENCE   1  (bases 1 to 1088)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1088)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:19:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,224
            CDSs (total)                      :: 3,198
            Genes (coding)                    :: 3,184
            CDSs (with protein)               :: 3,184
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1088
                     /organism="Aminobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_162697_length_1088_cov_3.706680"
                     /isolate="AMR_MDS_3026"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:3034609"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     regulatory      <1..75
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            139..951
                     /gene="thiD"
                     /locus_tag="P5201_15545"
     CDS             139..951
                     /gene="thiD"
                     /locus_tag="P5201_15545"
                     /EC_number="2.7.1.49"
                     /EC_number="2.7.4.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013047666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008902 - hydroxymethylpyrimidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0008972 - phosphomethylpyrimidine kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional hydroxymethylpyrimidine
                     kinase/phosphomethylpyrimidine kinase"
                     /protein_id="HRW00014.1"
                     /translation="MSVYRGIAVSIAGSDSGGGAGIQADLKTCAALKVFGMTVITALT
                     AQNSTAVTDVWNVPANMVRAQMDALWSDFPVAAAKTGMLSSPEIIRTVVQGVRQWSVT
                     NLVVDPVMVAQSGASLIADDAVEALREELLPHALLVTPNIPEAERLAGFSIRSTEEME
                     RASRRIAELGPKAVLVKGGHLLREDSVTDVFFYKGELTLFEDRRIFTENTHGTGCTLS
                     AAITAELAAGIPLEEAVRRGRQYLRMGLEASFRPGKGWGPLGHAVTPPWLRS"
BASE COUNT          213 a          279 c          368 g          228 t
ORIGIN      
        1 gagaggaagg cggaggcctt cgaccctcta cctgatccgg ataatgccgg cgtagggagc
       61 ggctcttttc agcgagcctg gagaaggtcc gttcattgcg aacgggcctt ttttatttga
      121 ggaaaggcgg aatgtgtgat gagtgtgtat cggggaatag cggtgtccat cgcgggaagc
      181 gattcaggag ggggcgcggg aatacaagcc gatctgaaga cgtgcgccgc tttgaaggtg
      241 ttcggcatga cggtgatcac agccttgaca gcgcagaaca gcactgccgt cacggatgta
      301 tggaatgtcc ctgcaaatat ggtccgcgcg cagatggacg ccctctggtc ggactttccg
      361 gtcgccgccg caaagacggg gatgctgagc tcgcccgaga tcatacggac ggtggtgcag
      421 ggtgtgcgac aatggagtgt gacgaatctc gtggtcgatc ccgtcatggt cgcacagagc
      481 ggcgcgtcgc tcatcgcgga tgacgccgtg gaagcgctca gagaggaatt gcttcctcat
      541 gcgctccttg tgaccccgaa tattcccgag gctgaacgtc tggccggttt ttcgatccgt
      601 tccacggagg agatggaacg cgcgtcgagg agaatagcgg agctaggccc gaaagccgtg
      661 ctggtaaagg gcgggcatct tctccgcgag gattccgtaa cggatgtttt cttttataaa
      721 ggggagctca cgctctttga agaccggagg attttcacgg aaaatacgca cggcacggga
      781 tgcacgctga gcgcagcgat taccgccgag cttgcggccg gtatcccctt ggaggaagct
      841 gttcgccggg gaaggcagta tctgcgaatg gggttggagg cgagtttccg ccccggaaaa
      901 gggtggggac ctctggggca tgcggtgact ccgccatggc tccggtcttg aaaatagtcc
      961 ccgaagactg ggcggaggcg ctgagtgtct gccgtgctcg ccgtcccctg atttacgccg
     1021 tcacgaatta tatcgccgcg tctttccaag cgaacgcgat cctcgcggtt ggggcttctc
     1081 ctgttatg
//
LOCUS       DAOTIL010001049         1087 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminobacteriaceae bacterium isolate AMR_MDS_3026
            NODE_163043_length_1087_cov_1.716085, whole genome shotgun
            sequence.
ACCESSION   DAOTIL010001049 DAOTIL010000000
VERSION     DAOTIL010001049.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868050
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminobacteriaceae bacterium (wastewater metagenome)
  ORGANISM  Aminobacteriaceae bacterium
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae.
REFERENCE   1  (bases 1 to 1087)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1087)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:19:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,224
            CDSs (total)                      :: 3,198
            Genes (coding)                    :: 3,184
            CDSs (with protein)               :: 3,184
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1087
                     /organism="Aminobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_163043_length_1087_cov_1.716085"
                     /isolate="AMR_MDS_3026"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:3034609"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..453)
                     /locus_tag="P5201_15550"
     CDS             complement(<1..453)
                     /locus_tag="P5201_15550"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRW00015.1"
                     /translation="MNRSPSGSRETNVRPYTEEAPFILIAQDFESLKSEVERLRTELS
                     MLVLERDELLFVECKNIETVYMLAIGVLEYKAYEIECAVLRLKRKAEMIQAKRNRQEP
                     VVLSKIEELLDIAFAEYQAKLDERIEKMNAAIERSRGAFLSDEEARELK"
     gene            complement(473..754)
                     /locus_tag="P5201_15555"
     CDS             complement(473..754)
                     /locus_tag="P5201_15555"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRW00016.1"
                     /translation="MASKFVSSWFCVSLEKHQDTFLSFPTGTVQIRAKCGEFADALRT
                     LHENFDAQGYDVVSVVPLSVGYAESCGSKDETVSFSVTSGAVVVGKLRE"
BASE COUNT          221 a          315 c          292 g          259 t
ORIGIN      
        1 cttgagttcg cgggcctcct cgtcggagag aaacgctccc cggcttcgtt cgatggcggc
       61 gttcattttt tcgatccgct cgtccaactt cgcttgatat tcggcgaacg cgatatcgag
      121 aagctcttcg attttggaga ggacaaccgg ttcctggcgg ttcctcttcg cctgtatcat
      181 ctccgccttt cttttcagcc gcagtacggc gcactcgatc tcgtacgcct tgtattcgag
      241 gacgccgatg gcgagcatgt acaccgtctc tatgttcttg cactcgacga acagcagctc
      301 gtcccgttcg agcacgagca tggagagttc cgtgcgaagc cgttccactt cggacttcag
      361 gctttcgaag tcctgcgcaa tgagaatgaa gggggcttcc tcggtatagg gtcttacgtt
      421 cgtttcgcgg cttccggacg gcgaccggtt catgcggact ctcgcggctt tcttattcgc
      481 ggagcttgcc gacgaccacg gcgccggagg tcacggaaaa gcttaccgtc tcatccttcg
      541 aaccgcagga ctccgcgtaa cctaccgaca acggcacgac gctcaccacg tcgtacccct
      601 gagcatcgaa gttttcgtgc agagtccgca gagcgtccgc gaactcgccg cactttgccc
      661 ttatctgtac ggttcccgta ggaaaggaga gaaacgtgtc ctggtgtttt tccagcgaaa
      721 cgcagaacca ggaagaaaca aacttgctcg ccactgtaca tcccccctgt gattctgata
      781 taaaacgaaa gacgctcttc cgacgtcgaa aaagaggcgc ctccgctccg cggataaggg
      841 aagagcacga tttaaggaat cgttttttcg aagacatgcg atgcggggac agcgtcgatc
      901 tcccgttttg tttgttcgcc cggaagcggt ttgctcggtt ccgccgaagc ctgcccgatg
      961 ctcccggatg gtcgatcgcg gccttcgggt ctttcgatgc acgacaacac atcattatag
     1021 tccgaaaaaa tgcctgaaat cgattcggct tgaggagact ttcaagcgag tctataatgg
     1081 atgcgga
//
LOCUS       DAOTIL010001050         1086 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminobacteriaceae bacterium isolate AMR_MDS_3026
            NODE_163100_length_1086_cov_3.935015, whole genome shotgun
            sequence.
ACCESSION   DAOTIL010001050 DAOTIL010000000
VERSION     DAOTIL010001050.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868050
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminobacteriaceae bacterium (wastewater metagenome)
  ORGANISM  Aminobacteriaceae bacterium
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae.
REFERENCE   1  (bases 1 to 1086)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1086)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:19:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,224
            CDSs (total)                      :: 3,198
            Genes (coding)                    :: 3,184
            CDSs (with protein)               :: 3,184
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1086
                     /organism="Aminobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_163100_length_1086_cov_3.935015"
                     /isolate="AMR_MDS_3026"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:3034609"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..355
                     /locus_tag="P5201_15560"
     CDS             <1..355
                     /locus_tag="P5201_15560"
                     /inference="COORDINATES: protein motif:HMM:TIGR03915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="TIGR03915 family putative DNA repair protein"
                     /protein_id="HRW00017.1"
                     /translation="LRFTEIGGVLYAPFEPEADLLQLLAGHFSTRLGNEKWIIHDLGR
                     GKAAIYAPRLWRIADFSLPEGIAPSRREEAFRDLWKEYFHSIAVRSRENPRLQRQFMP
                     KKYWKHLPEKEPPAE"
     gene            442..>1086
                     /locus_tag="P5201_15565"
     CDS             442..>1086
                     /locus_tag="P5201_15565"
                     /EC_number="5.3.1.12"
                     /inference="COORDINATES: protein motif:HMM:NF014652.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008880 - glucuronate isomerase activity
                     [Evidence IEA];
                     GO_process: GO:0006064 - glucuronate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucuronate isomerase"
                     /protein_id="HRW00018.1"
                     /translation="MAKKFLDKDFLLHSKAAQKLYHEYAAPMPIFDYHCHIPAEEIAG
                     DTQFASLAEAWLGGDHYKWRAMRACGVDERFVTGDASPEEKFAAWAKVVPQTIGNPLY
                     HWTHLELQRIFGVETLLNADTAAEIHDHCSKMLRKKEFSVRGLIRKFNVAALCTTDDP
                     ADSLEHHEAIKKSGFETLVAPAFRPDRALDAHIPDQFNPWLDSLAELTDVKVDSF"
BASE COUNT          249 a          350 c          289 g          198 t
ORIGIN      
        1 tctccgcttc accgagatcg gcggcgtgct ctacgctccg ttcgagcccg aggccgatct
       61 ccttcagctg ctcgccgggc acttctcaac gaggttagga aacgagaagt ggatcatcca
      121 cgacctcggg aggggaaaag cagcgatcta cgctccgcgc ctctggcgca tcgccgactt
      181 cagcctcccc gaaggaatcg ccccgtcccg ccgggaggaa gcattccgcg acctgtggaa
      241 agagtatttc cactccatcg ccgtccggtc gagggagaac ccgagactcc agagacagtt
      301 catgccgaaa aaatattgga aacaccttcc ggaaaaggag cctcccgccg aatagttcct
      361 acgatttccc cgtgcaaaaa ttcttatcat agagtaaaat acattttcag agtatgaaac
      421 catttgcaag gaggagacgc tatggcgaaa aagtttctgg ataaggattt ccttctgcat
      481 tccaaggcgg cgcaaaagct ctaccacgaa tacgccgccc cgatgccgat attcgactac
      541 cactgccata ttcccgcgga ggaaatagcg ggcgatacac agttcgcctc ccttgccgag
      601 gcgtggctgg gaggagatca ctacaagtgg cgcgctatga gggcgtgcgg cgtcgacgag
      661 cgcttcgtca ccggcgacgc ctcccccgag gagaagtttg ccgcgtgggc gaaggtagtt
      721 cctcagacca tcggcaaccc gctctaccac tggacgcacc tcgaactgca gcgaatcttc
      781 ggcgtcgaga cgctgttgaa cgcggacacg gccgccgaaa ttcacgacca ctgttcgaag
      841 atgctccgga agaaggagtt ctcggtccgc gggctgatcc gaaaattcaa cgtcgccgcc
      901 ctgtgtacca ccgacgaccc cgccgactcg cttgaacacc acgaggccat caaaaagagc
      961 ggtttcgaaa cgcttgtcgc gcccgccttc cgccccgacc gcgccctcga cgcacacatc
     1021 ccggaccagt tcaatccgtg gctcgactcg ctcgccgaac tcaccgacgt aaaagtggac
     1081 tccttc
//
LOCUS       DAOTIL010001051         1085 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminobacteriaceae bacterium isolate AMR_MDS_3026
            NODE_163348_length_1085_cov_3.666990, whole genome shotgun
            sequence.
ACCESSION   DAOTIL010001051 DAOTIL010000000
VERSION     DAOTIL010001051.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868050
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminobacteriaceae bacterium (wastewater metagenome)
  ORGANISM  Aminobacteriaceae bacterium
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae.
REFERENCE   1  (bases 1 to 1085)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1085)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:19:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,224
            CDSs (total)                      :: 3,198
            Genes (coding)                    :: 3,184
            CDSs (with protein)               :: 3,184
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1085
                     /organism="Aminobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_163348_length_1085_cov_3.666990"
                     /isolate="AMR_MDS_3026"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:3034609"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(461..>1085)
                     /locus_tag="P5201_15570"
     CDS             complement(461..>1085)
                     /locus_tag="P5201_15570"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HRW00019.1"
                     /translation="VETACDGLTALERFRAVRPDLVLLDVMMPGMNGYDVCRAIRTED
                     RFIPLLMLTAKSSEIDKVIGLELGADDYIVKPFGMAELLARVRSALRRGALSSTARTE
                     GAASSTLSFGDVTVNFESMTGQKNGVEFALTPKEAALLRFLTEREGKVVSRNTLLDDV
                     WGIDCEVTTRTVDQHVVRLRQKIEDDPSRPVFLCTVHGAGYRFVLEQ"
BASE COUNT          252 a          325 c          273 g          235 t
ORIGIN      
        1 aaaacccctt ctcgaaacgt tcgagaatcg tctcccgtaa agcggcggaa gggaaaagga
       61 cctcggctgc gggcatgctt ccaaaagcct cgctgccctc gacgccgaag aggcgggcaa
      121 agagcggatt gcactccaga agacgcctga aagatgcgtc ggtcatgaag aaagggtaat
      181 attcctgcgt actcagattt tgccaggata tggcgatagg gcggggagtc gtcctcttca
      241 aatgccccga agagaacgga gagttttcgt cagcgtgcat tccatgaaca cgtgccacac
      301 atccaccccc aggtaatgtt tcattctttg aaacaattcc cttctttaca tactctccac
      361 tatgcttatt aaaataccac attggaaatg attcaggaat gcctcttcga tgattcgcag
      421 gaatcatctt tgaacatctt tgcatgtaaa aacgttcttc tcattgttcc agaacgaatc
      481 gataccccgc cccgtgcaca gtgcaaagaa agaccggcct cgaggggtcg tcctcaatct
      541 tttgccgtaa ccggaccacg tgctggtcga cagtccgcgt cgtcacctcg cagtcgatcc
      601 cccacacatc gtccagaaga gtgtttctgg agacgacctt tccctctcgc tccgtgagaa
      661 aacgaagcaa cgccgcctct ttcggagtca gcgcaaactc aacgccgttc ttttgcccgg
      721 tcatgctctc gaaattcacc gtaacatccc cgaacgagag tgttgaagag gctgctccct
      781 ctgtccgcgc cgttgaggac agagcgcctc ttctcagcgc cgaccggacg cgggccaaaa
      841 gctcggccat tccgaacggt ttgacgatat agtcgtcggc cccgagttcg agcccgatca
      901 ccttgtcgat ctccgagctc ttggcagtaa gcatcagcag cggaatgaag cggtcttccg
      961 tacggatggc gcggcagacg tcgtatccgt tcattccggg catcatcaca tccaggagca
     1021 caaggtcggg ccgcacggcc cggaatcttt cgagcgccgt aagcccgtcg caggcggtct
     1081 ccact
//
LOCUS       DAOTIL010001052         1084 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminobacteriaceae bacterium isolate AMR_MDS_3026
            NODE_163715_length_1084_cov_1.965015, whole genome shotgun
            sequence.
ACCESSION   DAOTIL010001052 DAOTIL010000000
VERSION     DAOTIL010001052.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868050
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminobacteriaceae bacterium (wastewater metagenome)
  ORGANISM  Aminobacteriaceae bacterium
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae.
REFERENCE   1  (bases 1 to 1084)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1084)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:19:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,224
            CDSs (total)                      :: 3,198
            Genes (coding)                    :: 3,184
            CDSs (with protein)               :: 3,184
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1084
                     /organism="Aminobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_163715_length_1084_cov_1.965015"
                     /isolate="AMR_MDS_3026"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:3034609"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..401)
                     /locus_tag="P5201_15575"
     CDS             complement(<1..401)
                     /locus_tag="P5201_15575"
                     /inference="COORDINATES: protein motif:HMM:NF025056.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003953 - NAD+ nucleosidase activity
                     [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="toll/interleukin-1 receptor domain-containing
                     protein"
                     /protein_id="HRW00020.1"
                     /translation="MEIKCTKCGAADPMYSKKRGVYVCEECGHEFAREKPFEPRRIFI
                     SYGHDEHMALAVRRRDDLRERGHEAWFDEERLQPGHDWEGSIEKGLSWAAEVKPRAAV
                     VLLLTPHSVRRPDGYCLNEVARALALGLRIIP"
     gene            887..>1084
                     /locus_tag="P5201_15580"
     CDS             887..>1084
                     /locus_tag="P5201_15580"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRW00021.1"
                     /translation="MSKKIVKNRLWLDRMLNAPLHWQLALVAGLFFLVLLGGWSGIAW
                     LYGNAEIVLGEEALTPFWLTLL"
BASE COUNT          230 a          304 c          319 g          231 t
ORIGIN      
        1 gggatgatgc ggagtccgag agcgagtgcg cgggcgactt cgttcaggca gtagccgtcg
       61 gggcggcgta ccgagtgggg cgtcaggagg agcaccacgg ctgcgcgggg cttcacctcc
      121 gccgcccagg agagcccctt ctcgatgctc ccctcccagt cgtgaccggg ctgcaaccgc
      181 tcctcgtcga accacgcctc gtggcctcgc tcgcgcaggt cgtcgcggcg gcggacggcg
      241 agggccatgt gttcgtcgtg gccgtagctg atgaagatgc gccgcggctc gaacggtttt
      301 tcgcgcgcga attcatgccc gcactcctcg cagacgtaca ctccccgctt cttgctgtac
      361 atcgggtcag cggcgccgca cttggtacac ttgatctcca tgatgccatt cctccaatcg
      421 catgattcat tccagacgag aacccttgga agaaatcctt gtgcaacgga ggatcctcgt
      481 tgtcggtcag gaatgcgaaa aaatttgccg cactgtccca tatcggagcg gacaggcgat
      541 cccgctccat atcctttctt ggcatcgacc aatcaacttc tcgatagcgt cgttgttccc
      601 tgacgcggat aaaacaaaca ttttttggaa aagagacgat acctgacaaa tataccagaa
      661 gttcacccac gctcaaagaa gaatgacagg gacctgggga tcatttttct ccggcttgca
      721 ggtttgtctt gaaaggctcc tcgccgcata atgaatccga aggagctcgg gcagcttcga
      781 tgcggatgga caaagctccg gtggtagaat aaaacgaacc gatcgcacca acaatagaga
      841 caacatcggc actctgcatt tttcaagatg aaggagatct gcatacatgt cgaagaagat
      901 cgtcaagaac aggctctggc tggacaggat gttgaatgca ccgctgcact ggcagctcgc
      961 cttggtggcg ggactatttt ttcttgtgtt gctgggaggc tggagcggga tcgcatggct
     1021 ctacgggaac gccgagatcg tgctcggaga ggaggcgttg acgccgttct ggctgacgct
     1081 tctg
//
LOCUS       DAOTIL010001053         1084 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminobacteriaceae bacterium isolate AMR_MDS_3026
            NODE_163747_length_1084_cov_1.771623, whole genome shotgun
            sequence.
ACCESSION   DAOTIL010001053 DAOTIL010000000
VERSION     DAOTIL010001053.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868050
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminobacteriaceae bacterium (wastewater metagenome)
  ORGANISM  Aminobacteriaceae bacterium
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae.
REFERENCE   1  (bases 1 to 1084)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1084)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:19:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,224
            CDSs (total)                      :: 3,198
            Genes (coding)                    :: 3,184
            CDSs (with protein)               :: 3,184
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1084
                     /organism="Aminobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_163747_length_1084_cov_1.771623"
                     /isolate="AMR_MDS_3026"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:3034609"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..561
                     /locus_tag="P5201_15585"
     CDS             <1..561
                     /locus_tag="P5201_15585"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRW00022.1"
                     /translation="DPDSLLSDLIRSYNATLARLEEISGWKTGEKKPPILVLENFRGV
                     DIGIGQLRRVMPLETEAMHGIRVPVIGEMLRIKAWMVLTGNAMRDYIGFTALFTEVGD
                     SGFAEALGRFDSFYPQAEDAEPSALQLVRMLCEPMPYDLNDDGLAQYDQLSSPLTSWR
                     SISARCATTSGILFDVVMSQMGEGNR"
     gene            561..833
                     /locus_tag="P5201_15590"
     CDS             561..833
                     /locus_tag="P5201_15590"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRW00023.1"
                     /translation="MKHRHIHDDDDVMIAGIEDVIVRGDRFSQQRLFRRVVEDPFGDI
                     AERLQRVVESGNEEISSYLAVWGAFLARARSGTVWGMNPKNPPGGR"
BASE COUNT          244 a          271 c          317 g          252 t
ORIGIN      
        1 gatcccgaca gcctcttgtc cgacttgatc cgtagctata acgcaactct ggcgcggctc
       61 gaagaaatct ccggatggaa aacaggcgaa aagaagcctc ccatcctggt actggagaat
      121 tttcgcggag tagatatcgg catcggacag cttcgaagag tcatgccgct ggagactgag
      181 gcgatgcacg gtattcgagt acccgttatc ggcgagatgc tgcgcatcaa ggcctggatg
      241 gttttgaccg gaaacgcgat gcgggactac attggtttta ccgcactttt cactgaggtc
      301 ggagattccg gtttcgcgga ggctctcggg cgattcgata gcttctaccc ccaggctgaa
      361 gatgcggaac cttccgccct tcagcttgtc aggatgctgt gcgagcctat gccctacgat
      421 ctgaacgacg acggacttgc acagtacgat cagctctctt ctcctttgac atcgtggcgt
      481 tctatttcgg caaggtgcgc aaccacatcg ggcatcctgt tcgacgtggt tatgtcgcag
      541 atgggggagg gaaaccggta atgaaacacc gccatatcca cgacgacgat gacgtcatga
      601 tcgccggcat agaggatgtg attgtccgcg gggatcgttt ttctcagcaa aggctctttc
      661 gaagagtcgt tgaagaccct ttcggggata tcgcggaacg cttgcagcgc gtcgtggagt
      721 cgggaaacga ggagatatcc tcgtacctcg ctgtttgggg agcctttctc gcgagggcgc
      781 gaagcgggac ggtttggggt atgaatccca aaaatccgcc cgggggaaga tagcggagaa
      841 gactcgcaga acttgttcaa agtgtacgtc ctgttcttag cccgcttttc cgaagtcccc
      901 gtcgagcctt tttctccccg gaatttcgag ctgaagaaga gccggttgaa acagcagaga
      961 aaaaaactca ttccttcgcc gcccttagag attcctgtcc tggtattatc gacgggatga
     1021 aatttcagga gaagatgcgg gaggaatggg tgaagtggag tatcttttcg gtaccaacgc
     1081 agtt
//
LOCUS       DAOTIL010001054         1083 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminobacteriaceae bacterium isolate AMR_MDS_3026
            NODE_163809_length_1083_cov_4.495136, whole genome shotgun
            sequence.
ACCESSION   DAOTIL010001054 DAOTIL010000000
VERSION     DAOTIL010001054.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868050
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminobacteriaceae bacterium (wastewater metagenome)
  ORGANISM  Aminobacteriaceae bacterium
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae.
REFERENCE   1  (bases 1 to 1083)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1083)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:19:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,224
            CDSs (total)                      :: 3,198
            Genes (coding)                    :: 3,184
            CDSs (with protein)               :: 3,184
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1083
                     /organism="Aminobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_163809_length_1083_cov_4.495136"
                     /isolate="AMR_MDS_3026"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:3034609"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..794
                     /gene="yiaK"
                     /locus_tag="P5201_15595"
     CDS             <1..794
                     /gene="yiaK"
                     /locus_tag="P5201_15595"
                     /EC_number="1.1.1.130"
                     /inference="COORDINATES: protein motif:HMM:NF009750.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047559 - 3-dehydro-L-gulonate
                     2-dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="3-dehydro-L-gulonate 2-dehydrogenase"
                     /protein_id="HRW00024.1"
                     /translation="SFGALERWNGNRGMGCTNASSAMDRAMELAGLSGVGCVAMRNTN
                     HWMRGGAYGLQAARKGYIGICWTNTMPNMPPWGAKDRRIGNNPIIMALPWKESPVLID
                     GALAQFSYGAIEAHRLAGKQLPVPGGYDEEGRVTTDPAAIEKTWRVLPIGFWKGSGLS
                     IALDLIGALLSKGNTVRQIGELGDEIAVTQTFLAFNAEGMYGKEYVESVVEGVLSDLK
                     QAAPSEPGGEILYPGEKEGRTRRENLELGIPVNKEIWEGILRELA"
     gene            856..>1083
                     /locus_tag="P5201_15600"
     CDS             856..>1083
                     /locus_tag="P5201_15600"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRW00025.1"
                     /translation="MKKCTLLFLVAVLAALFVLPAQADPLKIVAAHNQTSLENPYQHG
                     MMKFKEVLEKISGGEISVDVHAGTIGTNEDEL"
BASE COUNT          226 a          330 c          341 g          186 t
ORIGIN      
        1 agtccttcgg cgcccttgaa cgatggaacg gcaacagagg catggggtgc acgaatgcgt
       61 cctccgcgat ggaccgcgct atggaactcg ccggtctctc cggcgtgggg tgcgtcgcca
      121 tgcggaacac gaatcactgg atgcgcggcg gggcctacgg ccttcaggcc gcgcgtaaag
      181 gctatatagg catctgctgg acgaacacca tgccgaacat gcctccatgg ggggcgaagg
      241 accgtcgcat cggcaacaac ccgatcatca tggcgcttcc gtggaaggag agccccgtgc
      301 tgatcgacgg cgcgctggcg cagttctcct acggggccat cgaagcccac cggctggcgg
      361 gcaagcagct tcccgtcccc ggcggctacg acgaagaggg acgggtcacg accgaccccg
      421 cggcgatcga gaaaacgtgg cgcgtgcttc cgatcggctt ctggaagggt tccggccttt
      481 ccatcgcgct cgacctgatc ggcgcgctcc tctcgaaggg gaacaccgtc cgtcaaatcg
      541 gcgaactcgg cgacgagatc gcggtcaccc agaccttcct cgccttcaac gcggagggaa
      601 tgtacgggaa ggagtacgtg gaatccgtcg tggagggcgt cctctccgac ctgaaacaag
      661 cggctccgtc ggaaccgggg ggcgaaatcc tctaccccgg cgagaaggag gggcgcaccc
      721 gcagggagaa cctcgaactg ggcatccccg tcaacaaaga gatctgggag ggtatcctcc
      781 gggagctcgc ttaaagttcg tgctttcgcg gaagtttttt ccgcatatct taaccaagag
      841 aaaggggtac caaaaatgaa gaagtgcaca ctgttgttcc ttgtagctgt tcttgcggcg
      901 ctctttgtcc ttcccgcgca ggccgatccg ctgaagatcg tggcggcgca caaccagacg
      961 tcccttgaga atccgtacca gcacggcatg atgaagttca aggaagtgct tgagaagatc
     1021 tccggcggcg aaatctccgt cgacgttcac gccggaacca tcggcaccaa cgaggacgaa
     1081 ctc
//
LOCUS       DAOTIL010001055         1082 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminobacteriaceae bacterium isolate AMR_MDS_3026
            NODE_164071_length_1082_cov_3.162610, whole genome shotgun
            sequence.
ACCESSION   DAOTIL010001055 DAOTIL010000000
VERSION     DAOTIL010001055.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868050
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminobacteriaceae bacterium (wastewater metagenome)
  ORGANISM  Aminobacteriaceae bacterium
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae.
REFERENCE   1  (bases 1 to 1082)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1082)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:19:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,224
            CDSs (total)                      :: 3,198
            Genes (coding)                    :: 3,184
            CDSs (with protein)               :: 3,184
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1082
                     /organism="Aminobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_164071_length_1082_cov_3.162610"
                     /isolate="AMR_MDS_3026"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:3034609"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(11..484)
                     /locus_tag="P5201_15605"
     CDS             complement(11..484)
                     /locus_tag="P5201_15605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008521045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicB family
                     antitoxin"
                     /protein_id="HRW00026.1"
                     /translation="MNTPDRYRYPAVLGYDVSTNQYYVLWPDLPGCTTTGATEDEALE
                     HAREAMSLHLWGMEVDGDEIPAPTPLHRLDLGEYVEEGEKIVAVLVEVWMPSFRERME
                     TKAVNRTVTLPGWLDSQAKRAALNYSQILQDGIMERLKISRVVVQRKKSGVKSLV"
     gene            complement(481..729)
                     /locus_tag="P5201_15610"
     CDS             complement(481..729)
                     /locus_tag="P5201_15610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016293326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003729 - mRNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicA family
                     toxin"
                     /protein_id="HRW00027.1"
                     /translation="MKQPEIDRNKIYSSSEIIKILEADGWQLHGAEGSHYQFKHPAKK
                     GKVTVKHPDKSVPGPTAKSIWKQAGLLQPPSSRKETES"
     gene            complement(822..>1082)
                     /locus_tag="P5201_15615"
     CDS             complement(822..>1082)
                     /locus_tag="P5201_15615"
                     /inference="COORDINATES: protein motif:HMM:NF016000.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhcH/YjgK/YiaL family protein"
                     /protein_id="HRW00028.1"
                     /translation="HRVYADVNYVAEGFEYLGYAPLERAGTPAVEYDPKTEAAFFEKE
                     CDFILLRKGDIAIVFPEDAHMPQKRALVPATVRKVCIKVRVG"
BASE COUNT          193 a          338 c          296 g          255 t
ORIGIN      
        1 tctccgcttc tcacaccaac gacttcactc cgctcttttt gcgctgtacg accaccctgc
       61 tgatcttcag acgctccatg ataccgtcct gaaggatctg cgaatagttc aaggcggcgc
      121 gcttggcctg actgtccagc cacccgggca gagtgaccgt tctgttcacg gccttcgtct
      181 ccatcctctc acggaaggac ggcatccaga cttcgaccag cacggcgacg atcttttcgc
      241 cctcctccac gtattcgccc aggtcgagcc gatgcaaggg ggtgggagcc gggatttcat
      301 ctccgtcgac ctccatgccc caaaggtgca gcgacatggc ttctctcgcg tgctccagcg
      361 cttcgtcttc ggttgcaccg gtcgtggtgc agccgggaag gtcgggccag aggacgtaat
      421 actgattcgt tgaaacatca taccccagaa cggcggggta gcggtatctg tcgggcgtgt
      481 tcatgactcc gtctcctttc gggaagaagg cggctgaagg agccccgctt gcttccaaat
      541 cgacttcgca gtaggaccgg ggacactttt atcggggtgc tttacggtta ccttgccctt
      601 ttttgcggga tgtttgaact gataatgact cccctccgct ccgtgcagct gccatccgtc
      661 agcttcgagg atcttgatga tttccgacga ggagtagatt ttattgcgat cgatctccgg
      721 ctgtttcatc cccggccacc ctttcagagc aaagtataac acgtgtaaaa aacatttgtc
      781 agcactgctc gtgatactgt tcttttcgct ctccgttcct ttcagccgac ccgcaccttg
      841 atgcagacct tgcggaccgt cgccgggacg agggcgcgct tctgcggcat gtgggcgtcc
      901 tcggggaaga cgatggcgat gtcgcccttc cgcagaagga tgaagtcgca ctccttctcg
      961 aagaatgccg cctccgtctt gggatcgtac tcgacggcgg gcgtaccggc gcgctcaagc
     1021 ggcgcgtacc ccagatactc gaacccctcg gcgacgtagt tcacgtcggc gtacacccga
     1081 tg
//
LOCUS       DAOTIL010001056         1079 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminobacteriaceae bacterium isolate AMR_MDS_3026
            NODE_164732_length_1079_cov_3.573242, whole genome shotgun
            sequence.
ACCESSION   DAOTIL010001056 DAOTIL010000000
VERSION     DAOTIL010001056.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868050
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminobacteriaceae bacterium (wastewater metagenome)
  ORGANISM  Aminobacteriaceae bacterium
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae.
REFERENCE   1  (bases 1 to 1079)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1079)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:19:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,224
            CDSs (total)                      :: 3,198
            Genes (coding)                    :: 3,184
            CDSs (with protein)               :: 3,184
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1079
                     /organism="Aminobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_164732_length_1079_cov_3.573242"
                     /isolate="AMR_MDS_3026"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:3034609"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..308
                     /locus_tag="P5201_15620"
     CDS             <1..308
                     /locus_tag="P5201_15620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008523407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="cysteine desulfurase NifS"
                     /protein_id="HRW00029.1"
                     /translation="NSNFSFIGIEGETLLLDLDGAGIYASTGSACSSGSLDPSHVLMA
                     IGLSHEQAHGSLRMTLGEETSEEDIRYVLSVLPAIVERRRAMSPLWEDFLKNTQGGK"
     gene            313..738
                     /gene="nifU"
                     /locus_tag="P5201_15625"
     CDS             313..738
                     /gene="nifU"
                     /locus_tag="P5201_15625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008678265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly scaffold protein NifU"
                     /protein_id="HRW00030.1"
                     /translation="MYSEKVLDHFQNPRNVGEIENPDGVGQAGNPKCGDIMKIYLRVE
                     NEVITDIKFKTFGCASAIASSSMATELVKGRNIDEAWNLTNSAVAEALDGLPPVKMHC
                     SVLAEEAIHAAINDYRRRKGLPVLEGKEYDHEHDHAHQH"
     gene            742..>1079
                     /locus_tag="P5201_15630"
     CDS             742..>1079
                     /locus_tag="P5201_15630"
                     /EC_number="3.2.2.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005660679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008725 - DNA-3-methyladenine glycosylase
                     activity [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase I"
                     /protein_id="HRW00031.1"
                     /translation="MNGAFNRCPWPGTDPLYVAYHDEEWGVPVREDATLFEFLLLEGA
                     QAGLSWITVLRKREAYRSAFDGFDPEIIAGYDERKIGELMRNEGIIRNKRKIEGAVKN
                     ARAFLRIRDR"
BASE COUNT          255 a          297 c          320 g          207 t
ORIGIN      
        1 acaactcgaa tttcagcttt atcgggatcg aaggcgaaac gcttcttctc gacctggacg
       61 gggccggtat ctacgcttcc accggcagcg cctgttcctc tggatcgctg gatccgtcgc
      121 acgttctcat ggcgatcggc ctttcccatg aacaggcgca cggttcgctc cgtatgacgc
      181 tcggggagga gacttcggaa gaggatatcc gctatgttct ctccgtgctt ccggcaatag
      241 tagagcgccg ccgcgctatg tcgcctctct gggaagactt tctgaagaac acgcaaggag
      301 ggaaataacg ctatgtacag cgaaaaagta ttggatcact ttcaaaatcc gcgcaacgtc
      361 ggtgaaatcg agaatccgga cggggtaggc caggcaggaa accccaagtg cggcgatatc
      421 atgaaaatat acctccgcgt ggagaacgaa gtaatcaccg acattaagtt caagacgttc
      481 ggctgcgcct ccgcgatcgc atcttcgagc atggcgaccg agcttgtgaa gggacggaat
      541 atcgacgaag cgtggaatct tacgaacagc gcggttgcag aggccctcga cggactgccc
      601 cctgtgaaga tgcactgttc cgtcctcgcg gaagaagcca ttcacgccgc aatcaacgac
      661 tatcgcagac ggaaggggct gcctgtgctc gagggtaaag agtacgatca cgagcacgat
      721 cacgctcatc agcactgagc cgtgaacggc gctttcaatc gctgcccgtg gccggggacc
      781 gaccccctct acgtcgcata ccacgatgag gagtggggcg ttcccgtccg cgaggacgcg
      841 acgctttttg aatttctcct attggaaggc gcgcaggccg gtctctcctg gatcactgtc
      901 ctccggaaac gggaagcgta caggagcgcg ttcgacggtt ttgatccgga gataattgcc
      961 gggtatgacg aacgaaaaat aggcgagctg atgcggaacg agggaatcat ccggaacaag
     1021 cggaagatag agggcgccgt gaaaaacgca cgcgccttcc tccggatcag ggatcggaa
//
LOCUS       DAOTIL010001057         1076 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminobacteriaceae bacterium isolate AMR_MDS_3026
            NODE_165431_length_1076_cov_3.260529, whole genome shotgun
            sequence.
ACCESSION   DAOTIL010001057 DAOTIL010000000
VERSION     DAOTIL010001057.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868050
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminobacteriaceae bacterium (wastewater metagenome)
  ORGANISM  Aminobacteriaceae bacterium
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae.
REFERENCE   1  (bases 1 to 1076)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1076)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:19:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,224
            CDSs (total)                      :: 3,198
            Genes (coding)                    :: 3,184
            CDSs (with protein)               :: 3,184
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1076
                     /organism="Aminobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_165431_length_1076_cov_3.260529"
                     /isolate="AMR_MDS_3026"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:3034609"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..369)
                     /locus_tag="P5201_15635"
     CDS             complement(<1..369)
                     /locus_tag="P5201_15635"
                     /inference="COORDINATES: protein motif:HMM:NF024116.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HRW00032.1"
                     /translation="MLETLRTAIRSLLSNKIRSALTMLGIIIGVAAVITMVAIGAGAS
                     RNIQQFISGVGSNILIIMPGAASVGGVRQQAGSGATLTTEDASALEREAYALKGVAPE
                     IYSRTQLVYGNMNWSTLVTGS"
     gene            complement(362..1054)
                     /locus_tag="P5201_15640"
     CDS             complement(362..1054)
                     /locus_tag="P5201_15640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005659909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HRW00033.1"
                     /translation="MSLIDVRDIVKIYRTGDVELRALDGVSFSVGQGEFVSIMGHSGS
                     GKSTMMNILGCLDVPDEGEYELDGERTGRLSKDALAKIRNAKIGFVFQGFNLLPRATA
                     LENVELPLIYAGVPGKKRKERGKTALERVGLADRMGHRPSQMSGGQQQRVAIARALVG
                     ETPLILADEPTGNLDTKTSAEIMDLFVALNREGKTIILVTHEPDIAEYSARTIRFQDG
                     RILADERRENHA"
BASE COUNT          156 a          356 c          362 g          202 t
ORIGIN      
        1 gctccccgtc accagggtgg accagttcat attgccgtag acgagctgcg tacggctgta
       61 aatctcgggg gccacgccct tcaatgcgta cgcctcgcgc tccagggcgg aggcgtcctc
      121 tgtggtgagc gtcgcgccgg agccggcctg ctgccggacg cccccgacgg aggccgctcc
      181 cggcatgatg atgaggatgt tgctgcccac gccggagatg aactgctgga tgttgcggct
      241 ggcccccgcg ccgatggcga ccatcgtgat cacggcggcg acgccgatga tgatgccgag
      301 catggtgagc gcggagcgta tcttgttgga gagcagcgag cggatcgcgg tgcggagcgt
      361 ctcaagcatg gttttctctc ctctcgtcgg cgaggatgcg cccgtcctgg aagcggatgg
      421 tgcgcgcgct gtactcggcg atgtccggct cgtgcgtgac gaggatgatg gtcttcccct
      481 cgcggttcag cgcgacgaag aggtccatga tctcggcgct ggtcttcgtg tccaggttgc
      541 ccgtaggctc gtcggcgagg atcagcggcg tctcgcccac aagtgcgcgg gcgatggcca
      601 cgcgctgctg ctgccccccg gacatctggc tcgggcggtg ccccatcctg tcggcgagcc
      661 ccacccgttc gagggcggtc ttcccccgct ccttgcgctt cttccccggc acgcccgcgt
      721 agatcagcgg cagttccacg ttttcgagcg ccgtggcgcg ggggaggagg ttgaacccct
      781 gaaagacgaa gccgatcttc gcgttgcgga tcttcgccag cgcgtccttc gagaggcggc
      841 ccgtccgttc tccgtccagc tcgtactccc cctcgtccgg cacgtcgagg cagccgagga
      901 tgttcatcat ggtggacttc cccgagccgg agtgccccat gatcgagacg aactccccct
      961 gcccgacgga gaaggagacg ccgtcgagcg cccgcagctc cacgtcgccg gtacggtaga
     1021 tcttgacaat gtcgcggacg tcgatcaggc tcattggaag atcctcttcc agagcg
//
LOCUS       DAOTIL010001058         1074 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminobacteriaceae bacterium isolate AMR_MDS_3026
            NODE_165942_length_1074_cov_2.889107, whole genome shotgun
            sequence.
ACCESSION   DAOTIL010001058 DAOTIL010000000
VERSION     DAOTIL010001058.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868050
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminobacteriaceae bacterium (wastewater metagenome)
  ORGANISM  Aminobacteriaceae bacterium
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae.
REFERENCE   1  (bases 1 to 1074)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1074)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:19:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,224
            CDSs (total)                      :: 3,198
            Genes (coding)                    :: 3,184
            CDSs (with protein)               :: 3,184
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1074
                     /organism="Aminobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_165942_length_1074_cov_2.889107"
                     /isolate="AMR_MDS_3026"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:3034609"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(137..622)
                     /locus_tag="P5201_15645"
     CDS             complement(137..622)
                     /locus_tag="P5201_15645"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRW00034.1"
                     /translation="MKINVRTPVILAVALLVALFVAGSVASASVQPVNFGQDHIKMQW
                     YLVNYGEENGVHFANARKYYTNPDVKAETIALIVEKFGTDQELANGLYFTEYGYAYSK
                     DGKSFAVTYINHYDMLGKVIRATEYDEASREFVGMSKNMIPFKAYAYATGKAKPAAKK
                     K"
     gene            complement(730..>1074)
                     /locus_tag="P5201_15650"
     CDS             complement(730..>1074)
                     /locus_tag="P5201_15650"
                     /inference="COORDINATES: protein motif:HMM:NF021507.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphodiester glycosidase family protein"
                     /protein_id="HRW00035.1"
                     /translation="GGPFLLRNGEVLSEREKLSDSIIDRRHPRTVVGLTKEGHWFFFV
                     GDGRNAVHSVGFTLDEVSRILKSAGVSYALNLDGGGSSGIISSGGFWNIPSDGLERPV
                     SYGIGAFQRRGR"
BASE COUNT          241 a          305 c          273 g          255 t
ORIGIN      
        1 gaattttctc ttctcctcca cacttctgcc ttcgaacatc tgtacattga cgaccggcat
       61 cgaaaaaacc tcctcggttc aaaatgaaac aaggcctggc ctcaggccag gccttgaaag
      121 tacgcagaaa aatcagctac ttcttctttg cggcgggctt cgcttttcca gtggcgtagg
      181 cgtacgcctt gaaggggatc atgtttttgg acatccctac aaattcgcgg gaagcctcgt
      241 cgtactcggt ggcgcggatg acttttccca acatgtcgta gtgattgatg tacgtgacgg
      301 cgaaagactt gccgtccttc gaataggcgt agccgtactc ggtgaagtac aatccgttgg
      361 caagctcctg gtcggtgccg aacttctcga cgatgagcgc gatggtctcg gccttgacgt
      421 ccggatttgt gtagtatttc ctggcgttcg cgaagtggac gccgttctct tcgccgtagt
      481 tcacgaggta ccactgcatc tttatgtggt cctgaccgaa gttgaccggc tgcaccgagg
      541 cggatgcaac ggaaccggcg acaaagagtg cgacaagaag agcgactgcc aaaataacgg
      601 gtgtacgtac attgattttc atgctgaatg agacaactcc ttccaatttt tcatctgcct
      661 tacgccgtgt agtctaccac aaaacggcga aaataaaacg cccggggagc attttcttcg
      721 ttcctttctc taccgccccc ttctttgaaa ggctccgatg ccgtaactca ccggacgctc
      781 caaaccatcg gaggggatat tccagaatcc cccgctggag ataatccccg aagaaccgcc
      841 tccgtcgagg ttcagcgcat aggaaacccc ggcgctcttc aggatgcggg agacctcgtc
      901 gagcgtgaag cctacgctgt gcacagcatt tcttccgtcg cctacgaaga aaaaccaatg
      961 cccctctttc gtcagcccga cgacagtcct ggggtgacgg cgatcgataa tcgagtcgct
     1021 caatttttct ctctcggaaa ggacttcccc gttccggaga aggaaagggc ctcc
//
LOCUS       DAOTIL010001059         1073 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminobacteriaceae bacterium isolate AMR_MDS_3026
            NODE_166225_length_1073_cov_2.161100, whole genome shotgun
            sequence.
ACCESSION   DAOTIL010001059 DAOTIL010000000
VERSION     DAOTIL010001059.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868050
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminobacteriaceae bacterium (wastewater metagenome)
  ORGANISM  Aminobacteriaceae bacterium
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae.
REFERENCE   1  (bases 1 to 1073)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1073)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:19:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,224
            CDSs (total)                      :: 3,198
            Genes (coding)                    :: 3,184
            CDSs (with protein)               :: 3,184
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1073
                     /organism="Aminobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_166225_length_1073_cov_2.161100"
                     /isolate="AMR_MDS_3026"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:3034609"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>1073
                     /locus_tag="P5201_15655"
     CDS             <1..>1073
                     /locus_tag="P5201_15655"
                     /inference="COORDINATES: protein motif:HMM:NF022812.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF3160 domain-containing protein"
                     /protein_id="HRW00036.1"
                     /translation="KAPNLKEVEEVVGKLTAEQKSALQKNRFLLLQKPSLHTFSSMGR
                     YDEMLCNFDGIGGSYDPAYREPWNTRFVGPDVFLHALHTFFSKRLEAMEGGELLGAVQ
                     YMLEELYANAVSLRSLASKSNKPHWERLQAQLVVPLILALNCDESVEPVWTDPDAEPA
                     QQTDSLKNALALFKKYGKSFSKKTADAVTEELQRIYAAEEALKGLLGLSPAYASQSVD
                     YTQFTPRGHYEKNSRSRAYFRTMIWLGQLGWKLADEEGAVDALNFALAMSYKRPVARG
                     KPQRPAPGALTTPLDAWKRVMEVSCFFVGYPDGASYPEWRQLLLKKAGVSSFTPDTCG
                     DAAAVDRIREASEQLAPSIPHFK"
BASE COUNT          220 a          354 c          322 g          177 t
ORIGIN      
        1 acaaggcgcc gaacctcaaa gaggtggagg aagtcgtcgg caagctcacg gccgagcaga
       61 agagcgcgct gcagaagaac cgctttttgc tgctgcagaa acccagtctg catacttttt
      121 cgtcgatggg gcggtacgac gaaatgctgt gtaacttcga tggcatcggg ggaagctacg
      181 atcccgccta ccgcgagccg tggaacaccc gcttcgtcgg cccggacgtc tttcttcatg
      241 cgcttcacac cttcttctca aagcgccttg aggcgatgga ggggggcgag ctcctgggcg
      301 cggtacagta catgctcgag gagctgtacg ccaacgccgt ctcgctccgt tcgctggcgt
      361 ccaagagtaa taaaccgcac tgggagcgac ttcaggcgca gctcgtcgtc cccctcatcc
      421 ttgccctcaa ctgcgacgaa agcgtcgaac cggtctggac cgatccggac gccgaacccg
      481 cgcagcagac cgacagtctg aaaaacgcgc tcgcgctctt caaaaagtac ggcaaatcct
      541 tctcgaagaa gacggccgac gccgttaccg aggaactgca acggatctac gccgcagagg
      601 aggcgctgaa agggcttctt ggcctcagtc cggcctatgc gagccagagc gtggactaca
      661 cccagttcac ccccagaggg cactacgaga agaactcgcg cagccgcgcc tacttccgga
      721 caatgatctg gctgggacag ctcggctgga aactcgccga cgaagagggc gccgtcgacg
      781 cgctcaactt cgccctcgcc atgtcgtata aacggccggt tgcccgaggc aagccccagc
      841 gcccggcgcc cggcgcgttg acaacgcccc tcgacgcgtg gaagcgcgtg atggaagtct
      901 cctgcttctt cgtcggctac cccgacggcg cgagctaccc ggagtggcgg cagcttctgc
      961 tgaagaaggc gggcgtctcc tccttcacgc cggacacctg cggcgacgcg gccgccgttg
     1021 accgaatcag ggaggcgagc gaacagcttg cgccctccat accccacttc aag
//
LOCUS       DAOTIL010001060         1072 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminobacteriaceae bacterium isolate AMR_MDS_3026
            NODE_166377_length_1072_cov_3.369715, whole genome shotgun
            sequence.
ACCESSION   DAOTIL010001060 DAOTIL010000000
VERSION     DAOTIL010001060.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868050
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminobacteriaceae bacterium (wastewater metagenome)
  ORGANISM  Aminobacteriaceae bacterium
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae.
REFERENCE   1  (bases 1 to 1072)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1072)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:19:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,224
            CDSs (total)                      :: 3,198
            Genes (coding)                    :: 3,184
            CDSs (with protein)               :: 3,184
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1072
                     /organism="Aminobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_166377_length_1072_cov_3.369715"
                     /isolate="AMR_MDS_3026"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:3034609"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>1072
                     /locus_tag="P5201_15660"
     CDS             <1..>1072
                     /locus_tag="P5201_15660"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HRW00037.1"
                     /translation="EEIVSILGARAGAKGLQLRLSMDPGVPASLRGDPLRLRQVLNNL
                     ADNAVKFTSRGEVSISVEPEEEGQNDVALRFSVRDTGIGIPLEKQGALFDKFSQVDTS
                     TTRKYGGTGLGLAISKKLVEMMGGEIGVTSAEGRGSEFWFSARFGKASGAGRALPQKE
                     HRDMTPLSAGQRRARLLVAEDNTTNLQVALGILKGLGLDADAASDGEEAVEAVQRHPY
                     DLVLMDCQMPRMDGYEATRRIRSLEGDVRDVPIIAMTAHAMAGDREKCLQAGMNDYVS
                     KPISPRLFVETLRRWLPSEADAPEDAPGLKETVGESCSTSSGQEREFASRVWDRSLLL
                     ERVLGDEALAEDVVAGFVSDMPE"
BASE COUNT          208 a          296 c          376 g          192 t
ORIGIN      
        1 cgaggaaatc gtctccatcc tgggagcgcg tgccggggcg aaaggcctgc aactgcgcct
       61 ttccatggat cccggcgtgc cggcctcgct gcggggagac ccgctgcgac ttcgacaggt
      121 gctgaacaac ctggcggaca atgcggtcaa gttcacttcg cggggcgagg tttccatctc
      181 cgtagagccc gaggaggaag gacaaaacga tgtcgcgctt cgcttttcgg tgcgcgacac
      241 ggggatcgga atcccgttgg agaagcaggg agcgctcttc gacaagttca gtcaggtgga
      301 tacctcgacg acaagaaaat acggaggcac cggccttggc ctcgctatct cgaaaaaact
      361 cgtcgagatg atgggcggag aaatcggcgt aacaagcgcc gaaggccggg gatcggagtt
      421 ctggttttcg gcgcggttcg gcaaggcgtc cggtgcgggg cgcgctcttc cgcaaaagga
      481 acaccgcgac atgacgccgc tctccgcggg gcaaaggcga gcgcggcttc tcgtcgcgga
      541 ggacaacacg accaacctcc aggtcgcgct gggcattctg aaaggcctgg gactggacgc
      601 ggacgcggcc tcggacggag aggaggctgt cgaggcggtg caacgccatc cgtacgacct
      661 ggtactgatg gactgccaga tgccccggat ggatggatac gaggcgacgc ggaggattcg
      721 ctcgctggag ggcgacgttc gcgacgtccc gatcatcgcg atgacggcgc atgcgatggc
      781 cggggacagg gagaagtgcc tgcaggcggg aatgaacgat tacgtttcca agccgatttc
      841 tcctcgtctc ttcgtcgaaa cgcttcggcg ctggcttcct tcggaggcag acgctcccga
      901 agatgctccg ggtctcaagg aaactgtcgg cgaaagttgc tcgacttctt cgggacagga
      961 gcgggagttt gcgtcccgtg tttgggacag gagcctcctg ctggagcggg tgctcgggga
     1021 tgaagcctta gccgaagatg ttgtcgcggg ctttgtgagc gatatgcctg ag
//
LOCUS       DAOTIL010001061         1072 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminobacteriaceae bacterium isolate AMR_MDS_3026
            NODE_166390_length_1072_cov_3.083579, whole genome shotgun
            sequence.
ACCESSION   DAOTIL010001061 DAOTIL010000000
VERSION     DAOTIL010001061.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868050
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminobacteriaceae bacterium (wastewater metagenome)
  ORGANISM  Aminobacteriaceae bacterium
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae.
REFERENCE   1  (bases 1 to 1072)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1072)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:19:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,224
            CDSs (total)                      :: 3,198
            Genes (coding)                    :: 3,184
            CDSs (with protein)               :: 3,184
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1072
                     /organism="Aminobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_166390_length_1072_cov_3.083579"
                     /isolate="AMR_MDS_3026"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:3034609"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..777)
                     /locus_tag="P5201_15665"
     CDS             complement(<1..777)
                     /locus_tag="P5201_15665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005661440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter large permease subunit"
                     /protein_id="HRW00038.1"
                     /translation="MAITIALLLIIVTLLLGLPVPFCFMLSTTFMVITHAYDPSFLLP
                     YAFSQMSSIVLLAIPLFIMVGGMMDRGGIGTSLVNFVDVFVGRVKGGLGVVAVVSCAV
                     FGSISGSCAATLSCIGSIMFPRLRENGYPMGHSAALVSCAAPLGLLIPPSSQMILFAW
                     VGQQSVLACFLATVGPGIVLMILLSLLNIIMLRNDPNVKVTPRLPFPETMQLLGNRGK
                     KALPALFMPFIVLGGIYGGIMTPTEAAAVAVLYSVPVGFWI"
     gene            complement(868..>1072)
                     /locus_tag="P5201_15670"
     CDS             complement(868..>1072)
                     /locus_tag="P5201_15670"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRW00039.1"
                     /translation="ILFTVWAVDMVWYSYVWMPRTTGLRIPVFISQTSVLVGYALMSL
                     YSVVYFFDDLFSFISMKKEGVSR"
BASE COUNT          272 a          302 c          330 g          168 t
ORIGIN      
        1 gatccagaac cccacaggca cggagtagag cacggcaacc gcggcagcct ccgtcggcgt
       61 catgatgcct ccgtagattc ctccgaggac gatgaacggc atgaagagcg cggggagcgc
      121 cttctttccg cggttcccca gaagctgcat cgtctctggg aagggtaaac ggggagttac
      181 tttcacgttc gggtcgtttc ggagcatgat gatattcaaa aggctgagaa gaatcatgag
      241 aacgatgccg ggtcccacag tggcaagaaa gcacgcgagc accgactgct ggcccaccca
      301 ggcgaagaga atcatctgcg aactcggcgg gatcagcagg ccgagcggag cggcgcatga
      361 aacgagcgct gcgctgtgcc ccatcgggta gccgttttcc ctgagacggg gaaacatgat
      421 cgagccgatg cacgagagcg tcgcggcgca gctccccgaa atggatccga agacggcgca
      481 ggagaccacc gccactacgc cgagcccccc cttgacgcgg ccgacgaaga cgtcgacgaa
      541 gttgacgagc gacgttccta tacctcccct gtccatcatc ccgcccacca tgatgaagag
      601 cggaatggcc agcagcacga tggagctcat ctggctgaag gcgtacggga gcaagaagga
      661 cggatcgtac gcatgggtaa tgaccatgaa ggtcgtggag agcatgaagc agaaggggac
      721 gggcagtccg agaagaagcg tcacgattat gagcagaagc gcaatggtta tcgccattcg
      781 tgtttcctcc agtgcgccgg ccgtccgctc cgccggaacg cggcgcgctc tcggggaaat
      841 gcgtggaccg gcagtaagat ggactcccta gcgggagact ccctccttct tcatcgaaat
      901 gaaggagaat agatcgtcga aaaaatagac gacggaatag agagacataa gcgcataccc
      961 tacgagcacc gatgtctgcg agataaagac aggtattctc aaaccggtcg tgcgcggcat
     1021 ccagacatag ctgtaccaca ccatgtcgac ggcccacaca gtgaagagaa ta
//
LOCUS       DAOTIL010001062         1071 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminobacteriaceae bacterium isolate AMR_MDS_3026
            NODE_166604_length_1071_cov_4.080709, whole genome shotgun
            sequence.
ACCESSION   DAOTIL010001062 DAOTIL010000000
VERSION     DAOTIL010001062.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868050
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminobacteriaceae bacterium (wastewater metagenome)
  ORGANISM  Aminobacteriaceae bacterium
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae.
REFERENCE   1  (bases 1 to 1071)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1071)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:19:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,224
            CDSs (total)                      :: 3,198
            Genes (coding)                    :: 3,184
            CDSs (with protein)               :: 3,184
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1071
                     /organism="Aminobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_166604_length_1071_cov_4.080709"
                     /isolate="AMR_MDS_3026"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:3034609"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            13..174
                     /locus_tag="P5201_15675"
     CDS             13..174
                     /locus_tag="P5201_15675"
                     /inference="COORDINATES: protein motif:HMM:NF020977.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GatB/YqeY domain-containing protein"
                     /protein_id="HRW00040.1"
                     /translation="MEHIVEEAGAFVKAAGPKDTGKVMGKVMAAVKGRADGKRVRSKV
                     HDYLQTLVE"
     gene            349..849
                     /gene="nrdR"
                     /locus_tag="P5201_15680"
     CDS             349..849
                     /gene="nrdR"
                     /locus_tag="P5201_15680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006300633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0140110 - transcription regulator activity
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator NrdR"
                     /protein_id="HRW00041.1"
                     /translation="MRCPSCGALETRVIETRTADEGRVVRRRRECPECSTRFTTYEKI
                     EEKRPLRVIKKDGSRETFGRDKIVRGISRACEKLPVSLEQIEDLATRIETDLKAEGYG
                     DIPVSLIGQRVMEGLKRISQVAYVRFASVYREFTDIESFQHEIARLIGEREKREGSGN
                     GEGSSE"
     gene            827..>1071
                     /locus_tag="P5201_15685"
     CDS             827..>1071
                     /locus_tag="P5201_15685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006300634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRW00042.1"
                     /translation="MEKDRLNDTPVLLKPKGDMAETSRKSGRQMLMFAPRGEATDLAL
                     MVDALGHEETHPWNAERILEALVKEAGVEPGLAGEIAA"
BASE COUNT          269 a          235 c          344 g          223 t
ORIGIN      
        1 tccgacgaag aattggaaca cattgtcgaa gaagcgggcg cgtttgtcaa agccgccgga
       61 cccaaggata cgggaaaagt gatggggaag gtcatggctg ccgtaaaagg ccgcgccgac
      121 ggtaaacgag ttcgttcaaa ggtgcatgac tatcttcaga cccttgtcga atgaccgttg
      181 caacacaatt gtttgagacc tagaaaagcc ggggaaaccc ggcttttttt attgtgcttt
      241 gcagtacgtc ttaaagtccc gtatgaaagg agtacaacgt ctctctggaa agatacacca
      301 tataggagta gtattgcgcg gtgattgcta tatatagggg gtgtgtctgt gcgatgtccg
      361 tcatgcggcg ctttggagac gcgggtaatc gagacgagga ctgcggatga agggcgtgtg
      421 gtccgccgga ggagggagtg tccggagtgc tccactcggt tcacaacata tgaaaaaatt
      481 gaggagaaac gtcctctccg ggtgataaaa aaggacggca gcagggagac gttcggtcgg
      541 gacaagattg tcaggggaat aagccgggca tgtgagaagc ttcctgtttc ccttgaacaa
      601 atcgaggatc tggcgacccg tattgaaacc gacctgaaag ctgaagggta cggagacatc
      661 cccgtgtccc tcatagggca gagagttatg gagggactga agcgaatcag tcaggtcgct
      721 tatgtccgtt tcgcctcggt ctacagggag tttacggata ttgaaagctt ccagcacgaa
      781 attgcccggc tcatcggcga acgggagaaa agggagggtt ccggcaatgg agaaggatcg
      841 tctgaatgat actccggtgc tgctgaaacc caagggcgat atggccgaga catccaggaa
      901 aagcggacgg cagatgctca tgttcgctcc tcggggtgag gcgaccgatc ttgcgcttat
      961 ggttgacgct cttgggcatg aggaaacgca cccctggaat gcggagcgga ttctcgaagc
     1021 tcttgtgaag gaagccggag ttgagcccgg gcttgccgga gagatcgcgg c
//
LOCUS       DAOTIL010001063         1070 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminobacteriaceae bacterium isolate AMR_MDS_3026
            NODE_166929_length_1070_cov_2.536946, whole genome shotgun
            sequence.
ACCESSION   DAOTIL010001063 DAOTIL010000000
VERSION     DAOTIL010001063.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868050
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminobacteriaceae bacterium (wastewater metagenome)
  ORGANISM  Aminobacteriaceae bacterium
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae.
REFERENCE   1  (bases 1 to 1070)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1070)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:19:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,224
            CDSs (total)                      :: 3,198
            Genes (coding)                    :: 3,184
            CDSs (with protein)               :: 3,184
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1070
                     /organism="Aminobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_166929_length_1070_cov_2.536946"
                     /isolate="AMR_MDS_3026"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:3034609"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..498)
                     /locus_tag="P5201_15690"
     CDS             complement(<1..498)
                     /locus_tag="P5201_15690"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRW00043.1"
                     /translation="MRTTVANRLLYYRNTVADTGRMNPDIATLRKENLLLLLSREEAM
                     SGVPEDAEAAKAFLERSLKTPREGASGGDDDGRVAGVLCPWIYTPRVVHGAAAEGAPF
                     ALLCVPAILSSEGRLLPSGEPPWIPRTVLQPLNSEGPTVGSVEDSDRFLASLESGSVE
                     TWEELA"
     gene            complement(812..>1070)
                     /locus_tag="P5201_15695"
     CDS             complement(812..>1070)
                     /locus_tag="P5201_15695"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRW00044.1"
                     /translation="NNAAAYRLIGEAKDARNDFAGAVEPYAKAVELEPGEPSHYFKLG
                     QALANIRALDEAKKIYSIMQQAFPNNKWTNQLEMAIRFMSR"
BASE COUNT          193 a          316 c          341 g          220 t
ORIGIN      
        1 ggcaagctcc tcccatgtct cgaccgaacc gctctccaag gaagcgagga aacggtcgga
       61 gtcctcgacc gatccgacgg tcggcccctc gctgttcagc ggctgcagga cggttctggg
      121 aatccaggga ggctcgccgg atggaaggag gcggccttcg gaggagagaa ttgccggaac
      181 gcagagaagg gcgaaggggg cgccctccgc agcggctccg tggacgacgc ggggggtgta
      241 gatccacggg cagagaacgc ctgcgactct tccgtcgtcg tccccgcccg aagcgccctc
      301 cctcggagtc ttcagggagc gttcaagaaa agccttcgcc gcctcggcgt cctccggaac
      361 gcccgacatc gcctcctcgc gggagaggag cagaaggagg ttctcctttc gaagcgttgc
      421 tatgtcaggg ttcattctgc cggtgtcggc gacggtgttc cggtagtaca agagccggtt
      481 cgcgacggtc gttcgcacgc gcgatcatcc tttcggggga gagatttcgt actattgtag
      541 ctcatcatgg aggatcgcac ggtgttccgg aaggcgactc aagcgatgtt cggaaggcct
      601 ctcgaagggg tgcgctcaag gagagtctgc gttttcgaga aagaagacaa gaaagggaag
      661 aatccccccc tcgtcttctt tctcgaataa accgataccg acggcttcgg ttcgtgacgc
      721 cgcgcggcgt tctttccaat cgaccgacga acgagagcat tccaaggatc ggtggggcgc
      781 aagggctctc tcgacgcttt tttcctctcc gctatcgcga catgaagcgt atcgccatct
      841 cgagctggtt cgtccacttg ttgttaggga aagcctgctg catgatcgaa tagatcttct
      901 tcgcctcgtc gagggcgcgg atgttcgcca gcgcctgccc cagcttgaag tagtgcgaag
      961 gctctcccgg ttccagttcc actgcttttg cgtacggctc cacggctccg gcgaaatcgt
     1021 tgcgggcgtc tttggcctcg cctatcaggc ggtacgcggc ggcgttgttc
//
LOCUS       DAOTIL010001064         1070 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminobacteriaceae bacterium isolate AMR_MDS_3026
            NODE_166932_length_1070_cov_2.525123, whole genome shotgun
            sequence.
ACCESSION   DAOTIL010001064 DAOTIL010000000
VERSION     DAOTIL010001064.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868050
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminobacteriaceae bacterium (wastewater metagenome)
  ORGANISM  Aminobacteriaceae bacterium
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae.
REFERENCE   1  (bases 1 to 1070)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1070)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:19:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,224
            CDSs (total)                      :: 3,198
            Genes (coding)                    :: 3,184
            CDSs (with protein)               :: 3,184
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1070
                     /organism="Aminobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_166932_length_1070_cov_2.525123"
                     /isolate="AMR_MDS_3026"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:3034609"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            165..986
                     /locus_tag="P5201_15700"
     CDS             165..986
                     /locus_tag="P5201_15700"
                     /inference="COORDINATES: protein motif:HMM:NF017941.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="QueT transporter family protein"
                     /protein_id="HRW00045.1"
                     /translation="MNEVFSMWKKTKMVVLVALCAGLYAALLIPFKGFVLIPGVTEFR
                     PANALPVVMGLLFGPAGAWGAAIGNLIGDFFGSLGIGSLFGFVGNFMFAYVPYKLWIN
                     LGLVSKQDKEPDLKSKRKIAAYVVVAILGSVACALVIAWGLEVLKLVPFAALASIISL
                     NNSLPSVVLGIPVLLVLYPRIKKWDLLWTDIMDEEDIPVAGGMSRIGGFFMTVSILGG
                     MIGGLMAAFGAGQAFLYKGFAAGGDVGSLGVLLVAGIGSVGLVLSSFIQSMPGKK"
BASE COUNT          172 a          276 c          366 g          256 t
ORIGIN      
        1 ggcgtttttc ttcaatatgg attcggtagc gtacggagcg tgtcttttca gcaaccttgc
       61 ggggagctga agcgcgccca tcgctgcgga gcgagggcgg agcgccgcgg ccttagcggc
      121 ggatatggcg aaacgcggag tcaaaccttg aaggagtggt ctttgtgaac gaagtattct
      181 cgatgtggaa aaaaacgaag atggttgtgc ttgtggcgct ctgcgccggt ttatacgcgg
      241 ccctgctgat ccccttcaag gggttcgtgc tcattccggg ggttacggag ttccgcccgg
      301 cgaacgccct tcccgtggtg atggggcttc tcttcggccc cgcaggagcg tggggggcgg
      361 cgatcggcaa tctcatcggc gactttttcg gctcgctggg tatcggcagt cttttcggct
      421 tcgtggggaa cttcatgttt gcgtacgtgc cgtacaagct ctggatcaac ctggggctgg
      481 tctcgaagca ggacaaggag cccgacctga agtcgaagcg gaagatcgcc gcgtacgtcg
      541 tcgtcgcgat tctggggtcg gtcgcctgcg cgctcgtgat cgcatggggg ctcgaggtgc
      601 tcaaactggt gccgttcgcg gcgctcgcga gcatcatctc gctgaacaac tccctcccct
      661 ccgttgtgct gggtattccc gtgctgctcg tgctctaccc caggatcaag aagtgggatc
      721 tcctctggac cgacattatg gatgaagagg acattcctgt agcgggcggc atgtccagga
      781 tcggcggttt cttcatgacg gtgagcattc tcggagggat gataggcggg cttatggcgg
      841 cgttcggcgc gggccaggcg tttctgtaca aggggttcgc cgcgggaggc gacgtcggtt
      901 cgctcggcgt actgctggtc gcgggaatcg gttcggtggg gctggtgctt tcgtcgttca
      961 tccagtcgat gccggggaag aagtaggttt tcgctctctt cggagccttt aaaagaaaag
     1021 gaggtgcgcc atgctgagat atggactgct tttttccgtg cagcgcttta
//
LOCUS       DAOTIL010001065         1065 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminobacteriaceae bacterium isolate AMR_MDS_3026
            NODE_168028_length_1065_cov_3.003960, whole genome shotgun
            sequence.
ACCESSION   DAOTIL010001065 DAOTIL010000000
VERSION     DAOTIL010001065.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868050
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminobacteriaceae bacterium (wastewater metagenome)
  ORGANISM  Aminobacteriaceae bacterium
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae.
REFERENCE   1  (bases 1 to 1065)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1065)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:19:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,224
            CDSs (total)                      :: 3,198
            Genes (coding)                    :: 3,184
            CDSs (with protein)               :: 3,184
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1065
                     /organism="Aminobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_168028_length_1065_cov_3.003960"
                     /isolate="AMR_MDS_3026"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:3034609"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..356
                     /locus_tag="P5201_15705"
     CDS             <1..356
                     /locus_tag="P5201_15705"
                     /inference="COORDINATES: protein motif:HMM:NF014643.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0110001 - toxin-antitoxin complex
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="type II toxin-antitoxin system Phd/YefM family
                     antitoxin"
                     /protein_id="HRW00046.1"
                     /translation="FQSYPMIQFSHKKRYLGNAQEGGISLKLATNVKPVGYLQENADE
                     IVRTLSERGEPLIITRDGEAKAVVQDIAEYERTQQTLALLKILSLGRRQIEEGKVRPA
                     EEVFSSLRKGLEECR"
BASE COUNT          248 a          268 c          277 g          272 t
ORIGIN      
        1 cattccagtc atatcccatg atacaattca gtcacaagaa acgttattta gggaatgccc
       61 aagagggggg gatttctttg aaattagcga ccaacgtaaa gccggtggga tatctgcagg
      121 agaatgccga tgagattgtt cgcactttaa gcgagcgagg cgaaccgctt attataaccc
      181 gcgacggcga ggccaaagcc gtagtgcagg atattgcgga atacgagcgg acgcagcaaa
      241 cgcttgcgct tctaaaaatt ctctctctgg gccgacgtca gatcgaagaa ggaaaggtcc
      301 gcccggcgga ggaggttttt tcatccctta ggaaaggtct cgaagagtgc cgatgacgta
      361 cgaggttttg gtcacggacg atgcttctcg cgaccttgcg gatatctgcg aaattatcgt
      421 tttgctccgt cgttctacgc tcgacgcgcg aaatcactgg ctgcgcgacc ccgcttacga
      481 tctccagttc acgccggctc atcccgcggt cctctcgcgc ccggatcatc tcgctgatta
      541 tctcgactct caggtcaagc tcccgccgtt cctctgtggt gaaaagacgt tccttaaagc
      601 tcgcccaatt ctcgcctgcc ggagagaata caatgttttt ttcatctgga atctccccaa
      661 gccgaagtgt tgtttcgagt tcggtcgccc ttgtctcaat tttccttcgg cgttctaatg
      721 gaagggactt catagtgtca ttgtgtgttc tcatttgggg cagtctcctt tcagagagca
      781 cagagagtct tggaaaattt cccccgaaag cggaatacag ttataaacgg cgaatgagac
      841 gaccttcccg aggagcagat tcgacgcata atcacggagg ttctgtcagg tggccttact
      901 gagtgaagaa aaagcgtgtc gtacttttag ggcggactct cctactcgaa caaagaaaga
      961 ttttggcggg atgctatttt gaggatttct ccgaccacca catcacggac tttggaagca
     1021 tctttgtacg cttgctttgc atcggtttcc acgagaggca ttgga
//
LOCUS       DAOTIL010001066         1063 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminobacteriaceae bacterium isolate AMR_MDS_3026
            NODE_168616_length_1063_cov_2.163690, whole genome shotgun
            sequence.
ACCESSION   DAOTIL010001066 DAOTIL010000000
VERSION     DAOTIL010001066.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868050
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminobacteriaceae bacterium (wastewater metagenome)
  ORGANISM  Aminobacteriaceae bacterium
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae.
REFERENCE   1  (bases 1 to 1063)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1063)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:19:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,224
            CDSs (total)                      :: 3,198
            Genes (coding)                    :: 3,184
            CDSs (with protein)               :: 3,184
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1063
                     /organism="Aminobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_168616_length_1063_cov_2.163690"
                     /isolate="AMR_MDS_3026"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:3034609"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..487
                     /locus_tag="P5201_15710"
     CDS             <1..487
                     /locus_tag="P5201_15710"
                     /inference="COORDINATES: protein motif:HMM:NF026098.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="pyrroline-5-carboxylate reductase dimerization
                     domain-containing protein"
                     /protein_id="HRW00047.1"
                     /translation="LGKRMRIARAMPNTAIAVRESMTCIASNGPDDKALEMAKLLFND
                     VGKTLRIREEDMLAATALGACGIAYFLRAIRAASQGGIEVGLSAKNSLAMAVQTAKGA
                     ACLLSVGGTHPESEIDKVTTPNGCTISGLNCMEHEGFSSAMIRGITVSAEKAARLYRK
                     E"
     gene            complement(521..>1063)
                     /locus_tag="P5201_15715"
     CDS             complement(521..>1063)
                     /locus_tag="P5201_15715"
                     /inference="COORDINATES: protein motif:HMM:NF013703.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015297 - antiporter activity [Evidence
                     IEA];
                     GO_function: GO:0042910 - xenobiotic transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HRW00048.1"
                     /translation="VAVYGVINRGLSFFFMPMMGIAQGFQPILGYNYGAGRLDRARSS
                     IRLAIITSTLLALAGFLTAQLFPAQIFAIFTRDEILIAEGSRAMRIITSMFFVIGFQI
                     VGSAMFQALGKARPAFILSLCRQVLLLIPFVLTLPPLLGSTDGVWLAFPLADALSCMV
                     TFFFFRRELRYHLIAGPEAA"
BASE COUNT          227 a          321 c          354 g          161 t
ORIGIN      
        1 gctcggcaag cggatgcgca tcgcccgcgc catgccgaac accgccatcg cggtgcggga
       61 gtcgatgacc tgcatcgcct ccaacggtcc ggacgacaag gcgctggaga tggcgaagct
      121 gctcttcaac gacgtgggga agacgctccg catccgcgag gaggacatgc tcgccgccac
      181 cgcgctgggc gcgtgcggca tcgcctactt cctgcgggcg atccgcgccg cgtcgcaggg
      241 cggcatcgaa gtcggcctct ccgcgaagaa ttccctcgcg atggccgtgc agactgcgaa
      301 gggagcggcg tgccttctct ccgtgggcgg tacgcacccg gagagcgaga tcgacaaggt
      361 gaccacgccg aacggctgca ccatctccgg attgaattgc atggagcacg aggggttcag
      421 ctccgcgatg atacgtggca tcaccgtctc cgcggagaag gccgcgaggc tctaccggaa
      481 agagtagcgc gaccttctcg acagtgaaaa ccccgttctc tcaggcggct tcggggccgg
      541 cgatcagatg gtaccgcagc tcccgccgga agaagaagaa cgtcaccatg caagagagcg
      601 cgtccgcgag cggaaaggcg agccacacgc cgtcggttga accgaggagg ggcggaagcg
      661 tcaggacgaa cgggatcaga aggagcacct ggcggcacaa tgagaggatg aatgcggggc
      721 gggcctttcc gagcgcctgg aacatcgccg agcccacgat ctggaagccg atgacgaaaa
      781 acatgcttgt gatgatgcgc atcgctcgtg atccctcggc gatgagtatc tcgtctcttg
      841 tgaagatggc gaatatctgc gccgggaaga gctgcgcggt aagaaaaccc gcgagcgcga
      901 gcagcgtcga ggtgattatc gccaagcgga tggagctgcg cgcgcgatcc aatcgccccg
      961 cgccgtagtt gtaccccagg atgggctgaa agccctgcgc tatgcccatc atcggcatga
     1021 aaaagaaaga gagtcctctg ttgattaccc cgtaaacggc tac
//
LOCUS       DAOTIL010001067         1062 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminobacteriaceae bacterium isolate AMR_MDS_3026
            NODE_168870_length_1062_cov_2.291956, whole genome shotgun
            sequence.
ACCESSION   DAOTIL010001067 DAOTIL010000000
VERSION     DAOTIL010001067.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868050
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminobacteriaceae bacterium (wastewater metagenome)
  ORGANISM  Aminobacteriaceae bacterium
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae.
REFERENCE   1  (bases 1 to 1062)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1062)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:19:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,224
            CDSs (total)                      :: 3,198
            Genes (coding)                    :: 3,184
            CDSs (with protein)               :: 3,184
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1062
                     /organism="Aminobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_168870_length_1062_cov_2.291956"
                     /isolate="AMR_MDS_3026"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:3034609"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..693)
                     /locus_tag="P5201_15720"
     CDS             complement(<1..693)
                     /locus_tag="P5201_15720"
                     /inference="COORDINATES: protein motif:HMM:NF019349.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HRW00049.1"
                     /translation="MPLLKNVPELRTKPMRDVIYEHLRKAIIRGELKADATFTDGEIA
                     EEYGVSRTPVREAVQKLEAEGYIERVPMKGNRICSVSPYELANSFAIRKALETLAVRY
                     AAVRITDEELDAMRDLLAQADKAFAEFSDNELLDRFFPIVKKFNKIAFEACRSERLAE
                     LVWAQRELFDRYMVMRIILPNRINKSLSRRKLLCEALSAHDPERAASVWTEHLDESFV
                     IWREKSGYAKELE"
     gene            851..>1062
                     /locus_tag="P5201_15725"
     CDS             851..>1062
                     /locus_tag="P5201_15725"
                     /EC_number="4.2.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012868776.1"
                     /note="Catalyzes the conversion of citrate to isocitrate;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aconitate hydratase"
                     /protein_id="HRW00050.1"
                     /translation="MGKNLVWKVIGEHLAGEAPEGGEVSLKIDHTLTQDTTGTMSYLQ
                     FEAIGVPRTRAERSVSYVDHNTLQAT"
BASE COUNT          216 a          286 c          319 g          241 t
ORIGIN      
        1 ctccagttcc ttcgcgtacc ccgacttctc gcgccagatg acgaacgact cgtcgagatg
       61 ctccgtccag acggaggccg ctctctccgg gtcgtgcgcc gaaagcgctt cgcagagcag
      121 tttccggcgg gagaggctct tgttgatcct gttgggaagg atgatccgca taaccatgta
      181 gcggtcgaag agttcccgtt gagcccagac gagctcggca agccgttcgg acctgcacgc
      241 ctcgaaggcg atcttgttga atttcttcac gatgggaaag aaacggtcga gcaattcatt
      301 atcgctgaac tcggcgaagg ccttgtcagc ctgcgcaaga agatcgcgca ttgcgtccag
      361 ctcctcgtcg gtgatgcgga cggcggcgta acgcaccgca agcgtttcga gggctttgcg
      421 tatggcgaag gagttcgcca gttcataagg agaaacggaa cagattcggt ttcccttcat
      481 cggaacccgt tcgatgtacc cttcggcttc cagtttttgg accgcctcac gtaccggggt
      541 gcggctgacg ccgtactctt cggcgatctc gccgtcggtg aacgtcgcgt ccgctttcag
      601 ttcgccgcgg atgatcgctt tccggaggtg ctcgtagatg acgtcgcgca tgggctttgt
      661 acgcagttcc gggacgttct tcaagagcgg cacgcgatca tctcctcgaa agtttttttg
      721 tttttttcca ataaaagttg gatccactat ccaacatcca gcatccatgt cgtataatag
      781 cccctggaag caaggctgta aagtgggtta atagactgta ttttgtatat ttgcacgagg
      841 gggcggagag atgggcaaga atcttgtctg gaaggtgatc ggggaacatc tcgcaggaga
      901 ggcgccggaa ggcggcgaag tttcgctgaa gatcgaccac acgctgacgc aggatacgac
      961 ggggaccatg tcgtatctcc agttcgaggc cataggcgtt ccgcggacac gcgcggagcg
     1021 ttcggtgagc tatgtcgacc acaacacgct gcaggcgacg tt
//
LOCUS       DAOTIL010001068         1060 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminobacteriaceae bacterium isolate AMR_MDS_3026
            NODE_169280_length_1060_cov_3.305473, whole genome shotgun
            sequence.
ACCESSION   DAOTIL010001068 DAOTIL010000000
VERSION     DAOTIL010001068.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868050
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminobacteriaceae bacterium (wastewater metagenome)
  ORGANISM  Aminobacteriaceae bacterium
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae.
REFERENCE   1  (bases 1 to 1060)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1060)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:19:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,224
            CDSs (total)                      :: 3,198
            Genes (coding)                    :: 3,184
            CDSs (with protein)               :: 3,184
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1060
                     /organism="Aminobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_169280_length_1060_cov_3.305473"
                     /isolate="AMR_MDS_3026"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:3034609"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..949
                     /locus_tag="P5201_15730"
     CDS             <1..949
                     /locus_tag="P5201_15730"
                     /inference="COORDINATES: protein motif:HMM:NF016629.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="Rpn family recombination-promoting
                     nuclease/putative transposase"
                     /protein_id="HRW00051.1"
                     /translation="RRFLRGSLPGDIDLDSLTVEDVELQNITFIPPDLSKNESDVLYK
                     VRRGSAEMYVYILVEHQSRVDFMMPFRLLSYMVEFWKRCTEQAGSRARRAGFLLPPVL
                     PVVFYEGASKWTAPVRFADKVESSSDFRGLVPDFEYLLISLRDKRSEDLLSFRDALGG
                     LCYLAAPSKKESFAEAAERLRMLLTVLSDEETELLTNHLRGYLRILMKKEGLEIDDAL
                     DLCLEREEADEMLTYMLKEFRKTRKEGREEGREEGREEGLEEGLEKGQLEMGISIALA
                     MLEAGEPEEKILRYTGFSSVQLAEIRDGRKSGGKKPSSR"
BASE COUNT          223 a          287 c          342 g          208 t
ORIGIN      
        1 tcggcggttt ctgagaggca gcctgcccgg cgacatcgac cttgactcgc tcaccgtcga
       61 agacgtcgag ctgcagaata tcaccttcat cccgcccgac ctctcgaaaa acgagtccga
      121 cgtcctctac aaggtgcggc ggggaagcgc ggagatgtac gtctacatac tggtggagca
      181 tcagtcccgt gtggacttca tgatgccgtt tcgcctgctt tcatacatgg tggagttctg
      241 gaaacgctgc actgagcagg ccggatcgag ggcgagaagg gccggatttc ttctcccacc
      301 cgttctgccg gtggtatttt acgagggagc ttccaaatgg acggctccgg tgcgtttcgc
      361 ggacaaggtg gagagttcgt ccgactttcg cggtctggtt cccgacttcg agtatctgct
      421 gatatcgctt cgggacaagc gctccgagga tttgctctca tttcgcgatg cgctcggcgg
      481 gctgtgctac ctcgcggccc cgtccaagaa ggagagcttt gcggaagccg cggagcgatt
      541 gcggatgctg ctgaccgtcc tgtccgacga ggagacggag ctgttgacga accatctgcg
      601 cggctatctg aggatactga tgaaaaagga aggcctggag atcgacgacg ctctggacct
      661 ctgtctggaa cgtgaggagg ctgacgagat gctgacgtac atgctgaagg agttcaggaa
      721 gactcgcaag gaaggccgcg aagaaggccg cgaagaaggc cgcgaagaag gacttgagga
      781 agggcttgag aaagggcagc tcgagatggg gatctccata gctctggcga tgctcgaagc
      841 gggcgagccg gaggagaaga tactgcgcta taccggcttc tcctccgtgc agttggcaga
      901 gatacgcgac ggccggaagt ccggcgggaa aaaaccctcg tccaggtaac ccgacctagt
      961 cggcgtgaag cagcaatgtg aacaaaggcg cgcccttcca atcggagagg aagagcgcgc
     1021 ttttgctctg tttcgtcccc tactcgtacc gcagcgcctc
//
LOCUS       DAOTIL010001069         1058 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminobacteriaceae bacterium isolate AMR_MDS_3026
            NODE_169827_length_1058_cov_3.460618, whole genome shotgun
            sequence.
ACCESSION   DAOTIL010001069 DAOTIL010000000
VERSION     DAOTIL010001069.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868050
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminobacteriaceae bacterium (wastewater metagenome)
  ORGANISM  Aminobacteriaceae bacterium
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae.
REFERENCE   1  (bases 1 to 1058)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1058)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:19:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,224
            CDSs (total)                      :: 3,198
            Genes (coding)                    :: 3,184
            CDSs (with protein)               :: 3,184
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1058
                     /organism="Aminobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_169827_length_1058_cov_3.460618"
                     /isolate="AMR_MDS_3026"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:3034609"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..316
                     /locus_tag="P5201_15735"
     CDS             <1..316
                     /locus_tag="P5201_15735"
                     /inference="COORDINATES: protein motif:HMM:NF040376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF6305 family protein"
                     /protein_id="HRW00052.1"
                     /translation="AAGIDKDQEIERVTGLFQSAKQKGMKLLIMHIGGEGRRGTLSDA
                     FIEAAAPHADRLIVVDGGNNDKIFDRLVEGKNVKILTAPNVNGTAEPLKATLSEWGVS
                     AQ"
     gene            332..>1058
                     /locus_tag="P5201_15740"
     CDS             332..>1058
                     /locus_tag="P5201_15740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006299784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter large permease subunit"
                     /protein_id="HRW00053.1"
                     /translation="MEWFWPEGFYTIVMVGSFVLGAFALKLPIAIALSGAAVVGALAG
                     GEWFPLRHFVEGMFGYLDTILIIASAMIFMKSVQKTGLLESLAAWVIRRFRRKPLLLS
                     VGLIFIIMAPGMITGSSTAAVLTTGALVAPVLIKLGVPAVKTAAAIAMGAIYGMIAPP
                     INIPAMIIGGGIDMPYVGFALPLLVCTIPLAIFSALLLVYPSLKSGAGDEDELENELR
                     RMEETPLSARLLLPVVLLAVLMTG"
BASE COUNT          196 a          309 c          321 g          232 t
ORIGIN      
        1 cgcggcgggc atcgacaagg accaggagat tgagcgagtg acgggactct tccagtcggc
       61 gaagcagaaa ggaatgaagc tcctcataat gcacatcggc ggcgaaggga gaagagggac
      121 gctctccgac gcttttatcg aagctgccgc tcctcacgcc gacaggctta ttgtggtcga
      181 cggcggaaac aacgacaaga ttttcgatcg tttggtcgag ggaaagaatg tcaaaattct
      241 caccgcgccc aatgtgaacg gcacggcgga gcctctcaag gcaaccctct cggagtgggg
      301 ggtatccgcc caataggcgg gaggccctgt catggagtgg ttctggcctg aaggattcta
      361 tacgatcgtg atggtgggga gctttgttct cggcgcgttt gcgctgaagc tccccatagc
      421 gatcgcactc tccggcgcgg cggtcgtcgg cgctctcgcc ggcggcgaat ggttccctct
      481 ccgccatttc gtggagggta tgttcggtta tctggatacg attcttatca tcgcctccgc
      541 gatgatcttc atgaaatcag tgcagaagac ggggttgctg gagagcctcg cggcgtgggt
      601 aatccgcaga ttccgccgca agccgcttct cctcagcgtc gggttgatct tcatcatcat
      661 ggcgcccggt atgatcaccg gttcgtcgac cgcggccgtt ctcaccacgg gagcccttgt
      721 cgctcccgtt ctgataaaac tcggcgttcc ggccgtcaag actgccgcgg cgatcgcaat
      781 gggcgctatc tacggtatga tcgcaccgcc tatcaacatc ccggcgatga tcatcggcgg
      841 aggcatcgac atgccgtacg tcggctttgc cctgcctctt ctcgtctgca caattcccct
      901 ggcaatcttt tcggcgctgc tcctcgtcta cccctccctg aaaagcggcg ccggcgatga
      961 ggatgaactg gaaaacgagc tgcggcggat ggaagagacg cctctctcgg cgcgtctttt
     1021 gcttcctgtt gtactcctgg cggtgctgat gaccggag
//
LOCUS       DAOTIL010001070         1056 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminobacteriaceae bacterium isolate AMR_MDS_3026
            NODE_170317_length_1056_cov_3.881119, whole genome shotgun
            sequence.
ACCESSION   DAOTIL010001070 DAOTIL010000000
VERSION     DAOTIL010001070.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868050
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminobacteriaceae bacterium (wastewater metagenome)
  ORGANISM  Aminobacteriaceae bacterium
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae.
REFERENCE   1  (bases 1 to 1056)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1056)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:19:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,224
            CDSs (total)                      :: 3,198
            Genes (coding)                    :: 3,184
            CDSs (with protein)               :: 3,184
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1056
                     /organism="Aminobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_170317_length_1056_cov_3.881119"
                     /isolate="AMR_MDS_3026"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:3034609"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..477
                     /locus_tag="P5201_15745"
     CDS             <1..477
                     /locus_tag="P5201_15745"
                     /inference="COORDINATES: protein motif:HMM:NF012245.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HRW00054.1"
                     /translation="EESNTAAKEIAALAAGITKDLDSVVTSSEQGAKASKDSSHLAEE
                     TRTTIGKMMEALSRISMATQDLAAVSEEQAASSEEIASAIQNIASRVSAAASSSDMVR
                     GQMAEVGASAERVAQGSEELAGLAEDLKKLVGGFKFEDATHKGLVPVGNAPKGKKR"
     gene            578..1042
                     /locus_tag="P5201_15750"
     CDS             578..1042
                     /locus_tag="P5201_15750"
                     /inference="COORDINATES: protein motif:HMM:NF013731.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheW"
                     /protein_id="HRW00055.1"
                     /translation="MAEQLLVVFALGKEEFGIDISRVREIVRMQNITTIPQSMDFVEG
                     IVNLRGQIVPIVDLCKRFLVVDREGRNDIDRRIIVVHMAGQNIGILVDGVSEILRVPD
                     EAIEPTPPMVASGASVDFIRGVAKVKDRLIMALDLDRIFSTEERELLAQTES"
BASE COUNT          240 a          286 c          360 g          170 t
ORIGIN      
        1 gaagagagca acacggccgc caaagagatc gccgcgctag ccgcagggat cacgaaggat
       61 ttggacagcg tcgtcacgtc gtcggaacag ggagcgaagg cgtcgaagga ctccagtcac
      121 ctcgccgagg aaacgcgcac gaccatcggg aagatgatgg aggcgctctc tcgtatctcg
      181 atggcgacgc aggaccttgc cgcggtgtcg gaagagcagg cggcgtcgag cgaggagatt
      241 gcgagcgcta tacaaaacat agcgtcccgc gtgagcgccg cggcctcctc ttcggatatg
      301 gtgcgcggtc agatggcgga ggtcggcgcc tcagcggaac gcgtagcgca gggttccgag
      361 gagctcgccg gacttgccga ggatctgaaa aagctggtcg gcggcttcaa gttcgaggac
      421 gctacgcaca aaggactggt tccggtaggg aacgccccca aagggaaaaa acgctgaccc
      481 ctccggggag ttacgcacac actccctcgc cgctcccggt gcaactgacg cgcccgggag
      541 cggcttgaaa ggaccgcatc gaaaaggagg aagagctatg gcggaacagc tgcttgtcgt
      601 ctttgcactg ggaaaagaag agttcggcat cgacatctcc agagtgcgcg agatcgtgag
      661 aatgcagaat atcaccacaa taccgcagtc tatggacttt gtggagggga tcgtcaacct
      721 ccgggggcag atcgttccca tcgtcgacct gtgcaagcgt tttttggtcg tcgacaggga
      781 gggaagaaac gatatcgacc ggcgtatcat cgtcgtccac atggcggggc agaacatcgg
      841 catcctggtg gacggcgtgt cggagatact gcgcgttccg gacgaggcca tcgagccgac
      901 gcccccaatg gtggcgagcg gcgcaagcgt ggacttcatc cgcggagtgg cgaaggtgaa
      961 ggatcgtttg atcatggcgc tggatctcga ccgtatcttc tcaacggaag agagggagct
     1021 gctcgcccag accgagagct gagagaacgc ggggga
//
LOCUS       DAOTIL010001071         1056 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminobacteriaceae bacterium isolate AMR_MDS_3026
            NODE_170344_length_1056_cov_3.071928, whole genome shotgun
            sequence.
ACCESSION   DAOTIL010001071 DAOTIL010000000
VERSION     DAOTIL010001071.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868050
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminobacteriaceae bacterium (wastewater metagenome)
  ORGANISM  Aminobacteriaceae bacterium
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae.
REFERENCE   1  (bases 1 to 1056)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1056)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:19:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,224
            CDSs (total)                      :: 3,198
            Genes (coding)                    :: 3,184
            CDSs (with protein)               :: 3,184
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1056
                     /organism="Aminobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_170344_length_1056_cov_3.071928"
                     /isolate="AMR_MDS_3026"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:3034609"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(148..660)
                     /gene="tpx"
                     /locus_tag="P5201_15755"
     CDS             complement(148..660)
                     /gene="tpx"
                     /locus_tag="P5201_15755"
                     /EC_number="1.11.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008521723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008379 - thioredoxin peroxidase activity
                     [Evidence IEA];
                     GO_function: GO:0016684 - oxidoreductase activity, acting
                     on peroxide as acceptor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiol peroxidase"
                     /protein_id="HRW00056.1"
                     /translation="MERRGITTMKGNPMTLVGPGLQPGDKAPDFLVLDQTMAPKTLKD
                     YAGKFKVISVTPSLDTPVCDLQIHWFNEDASKQPQDVAVLNISMDLPFAIKRFCATGG
                     IENAVALSDHRDASFGTNWGVLMKELRLLARSIFIVDKEDVVQYVQIVPEQTAEPDYE
                     AALAALKELM"
     gene            complement(777..>1056)
                     /locus_tag="P5201_15760"
     CDS             complement(777..>1056)
                     /locus_tag="P5201_15760"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRW00057.1"
                     /translation="VVSTGVSLIFGGARRIVGLWVKVKGSTVAGDRKVNIAASLIYVL
                     ITWGIALFGLIPLIAKGYGYTGVLSFPLLIVPVLLIGVMRSGKWGKEL"
BASE COUNT          208 a          299 c          317 g          232 t
ORIGIN      
        1 gctgttttct ccatgaggaa cacctccgcg ataattgtac ggctcttgta cggaagtgtc
       61 aacgataatc gcatgcgaca gggtaaatgg agtctccatc gctgtagagt gaaaggagcc
      121 cggatctctc cgggctcctg gtgcttctta cataagctcc ttcagtgcgg ccagggcggc
      181 ttcgtagtcc ggctcggcgg tctgctccgg gacgatctgg acgtactgga cgacgtcctc
      241 cttgtccacg atgaagatgg agcgggcgag gaggcgaagc tccttcatca ggacgcccca
      301 gttggttccg aaggaggcgt ctctgtggtc cgagagagcg acggcgttct ctatgccgcc
      361 ggtggcgcag aagcgcttga tggcgaaggg aaggtccatc gaaatgttga gcaccgccac
      421 gtcctgcggc tgcttcgatg cgtcctcgtt gaaccagtga atctggaggt cgcacacggg
      481 ggtatccagc gagggggtca ccgatatgac cttgaacttc cccgcgtagt ccttcagcgt
      541 cttcggggcc attgtctggt cgagcacgag gaaatccggc gctttgtctc ccggttgcag
      601 gccggggccg acgagcgtca tcggattccc cttcattgtg gttattcctc ttctttccat
      661 agcgtattca cctccgaaat aatttctgtg gcttattgta tcacaaaaag agggcggagg
      721 ggaatatacc cccccgccct gtatggcgcg aataaatgag gcgacttttc gactctctac
      781 agctctttgc cccacttccc ggagcgcatg acgccgatga gcagcaccgg gacgatgagc
      841 aacggaaagg agaggactcc ggtgtacccg taccctttgg cgatcagcgg aataagaccg
      901 aagagcgcga tgccccacgt gatgaggacg tagatcaggg atgctgcgat gttcactttt
      961 ctgtctccgg ccactgtcga tccctttact ttcacccaga gtcccacgat gcgccgcgcg
     1021 ccgccgaaga tgaggctgac gccggtggag accacg
//
LOCUS       DAOTIL010001072         1054 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminobacteriaceae bacterium isolate AMR_MDS_3026
            NODE_170868_length_1054_cov_2.336336, whole genome shotgun
            sequence.
ACCESSION   DAOTIL010001072 DAOTIL010000000
VERSION     DAOTIL010001072.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868050
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminobacteriaceae bacterium (wastewater metagenome)
  ORGANISM  Aminobacteriaceae bacterium
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae.
REFERENCE   1  (bases 1 to 1054)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1054)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:19:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,224
            CDSs (total)                      :: 3,198
            Genes (coding)                    :: 3,184
            CDSs (with protein)               :: 3,184
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1054
                     /organism="Aminobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_170868_length_1054_cov_2.336336"
                     /isolate="AMR_MDS_3026"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:3034609"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..309
                     /locus_tag="P5201_15765"
     CDS             <1..309
                     /locus_tag="P5201_15765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013048990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-dependent synthetase"
                     /protein_id="HRW00058.1"
                     /translation="RVTDIIIVGGFNVYPQEVEAILHTHPAVSQAVVVGMPHPVNGEV
                     PKAFIQLREGMQVTSREIIDFCKKHLAHFKVPRSIEFMESFPLSSTGKVLRRMLKQPK
                     "
     gene            complement(365..838)
                     /locus_tag="P5201_15770"
     CDS             complement(365..838)
                     /locus_tag="P5201_15770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013048989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferritin family protein"
                     /protein_id="HRW00059.1"
                     /translation="MTEKSFNMKDALRYGIHAEIEAKNFYHTWAESVQEEHLKKELSA
                     LSDWESEHEASLMNYYEKLFGAQCPIDPNMVVEPELHVQTKDFGDTTSLLRVASAAYL
                     SEMRAMEFYERLEKESTGETSAMFRKIKDMEKEHMDITKKRYMKIREDVVGFRAF"
     regulatory      956..>1054
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00174"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="cobalamin riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="adenosylcobalamin"
                     /db_xref="RFAM:RF00174"
BASE COUNT          215 a          311 c          281 g          247 t
ORIGIN      
        1 cgggtgaccg atatcatcat cgtcggcgga ttcaacgtgt acccccagga ggtcgaggcc
       61 attctccaca cccatccggc cgtatcccag gccgtggtgg tgggtatgcc gcaccccgtc
      121 aacggcgagg tgccgaaggc attcatccag cttcgcgagg gaatgcaggt tacgtcgcgc
      181 gagatcatcg acttctgtaa aaaacacctg gcgcacttca aggtgccccg aagcatcgag
      241 ttcatggagt cattcccgct ttccagcacg gggaaggtgc tccggagaat gttgaagcag
      301 ccgaagtaaa aaaccggggc ttcctcaatt tggggaagcc ccggttccct agccggaaaa
      361 aaactcagaa tgcccggaag ccgacgacgt cctcgcggat cttcatgtac cgtttcttcg
      421 tgatgtccat gtgctccttt tccatgtcct tgattttgcg gaacatggcg gaagtttcgc
      481 ctgtggactc cttttcgagg cgctcgtaga actccatcgc gcgcatctcg gagagatatg
      541 cggcggaggc gacgcggagc agcgatgtcg tgtcgccgaa gtccttcgtc tgaacgtgca
      601 gctccggttc tacgaccatg ttcgggtcga tggggcactg cgcgccgaag agcttttcgt
      661 agtagttcat cagacttgcc tcgtgttcgc tctcccaatc ggagagggcc gacagctctt
      721 tcttcaagtg ctcttcctgg acgctctccg cccacgtgtg gtaaaagttc ttcgcctcga
      781 tttcagcgtg gatcccgtag cggagcgcat ccttcatgtt aaaactcttt tctgtcatcg
      841 tcatccctcc tgtgcaagat tagattcttt cctgtattat accccattcc cgcaaaaatt
      901 ccaaaaacgc aggagctttt cctcgccttg acaaaccctt cccgtcggac tactatcaga
      961 acgaaggcgc gtgcgccttc tttcggaaga tgttcgggaa gtccggtgaa aatccggcgc
     1021 ggacccgcca ctgtaagcct gacgaagcca ccga
//
LOCUS       DAOTIL010001073         1053 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminobacteriaceae bacterium isolate AMR_MDS_3026
            NODE_171041_length_1053_cov_3.879760, whole genome shotgun
            sequence.
ACCESSION   DAOTIL010001073 DAOTIL010000000
VERSION     DAOTIL010001073.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868050
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminobacteriaceae bacterium (wastewater metagenome)
  ORGANISM  Aminobacteriaceae bacterium
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae.
REFERENCE   1  (bases 1 to 1053)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1053)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:19:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,224
            CDSs (total)                      :: 3,198
            Genes (coding)                    :: 3,184
            CDSs (with protein)               :: 3,184
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1053
                     /organism="Aminobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_171041_length_1053_cov_3.879760"
                     /isolate="AMR_MDS_3026"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:3034609"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..347)
                     /locus_tag="P5201_15775"
     CDS             complement(<1..347)
                     /locus_tag="P5201_15775"
                     /inference="COORDINATES: protein motif:HMM:NF024758.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine protease"
                     /protein_id="HRW00060.1"
                     /translation="MISRGCASLRLFSTLFLALLLWSGGAYAAGFPPAMIRDASRAVK
                     MLVTEGNGDRMGQGTGFFVTPDGLMLTNYHVVEGNRTIKIWDEKNLYDIVRIVAFDKN
                     ADVALLETNYPKSS"
     gene            451..>1053
                     /locus_tag="P5201_15780"
     CDS             451..>1053
                     /locus_tag="P5201_15780"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRW00061.1"
                     /translation="MTEQQATVQKGNGGRSGKRWFLPFLTIFAVLAYLSFRASFSLIG
                     PLAWSALISFTVYPLYIRMRRRFFSRRSANLPALLATAIIVFAVLIPCLLGLFFAARE
                     GVRLLDSTEELLTSMESSGEGAIATLLPEHIVSKIRPWMERYPTLRAYVRQTVARSAE
                     SAVDLVGSAILFLYYFSIITVANFFFLRDGHLIIGFLREIV"
BASE COUNT          187 a          341 c          304 g          221 t
ORIGIN      
        1 gacgacttcg gatagttcgt ctccagcagg gcgacgtcgg cgttcttgtc gaacgccacg
       61 atccggacga tgtcgtagag gttcttctcg tcccatatct tgatcgtcct gttcccctcg
      121 accacgtgat agttcgtcag catcagaccg tcgggcgtga cgaaaaagcc cgtcccctgc
      181 cccatgcggt cgccgttgcc ctccgtcacc agcatcttga cggcgcggga ggcgtcgcgg
      241 atcatcgcgg gcgggaatcc cgccgcgtac gcgcccccgc tccacagaag gagcgcgaga
      301 aagagcgtcg aaaaaagccg taacgaggcg cagcctcgtg aaatcataca catcaccccg
      361 aatcgtcgaa cggcggaggc gattcgccgc gccgagtggt atccttactc aaaagagaat
      421 accacaatcg gacgaaagcg ggtggcgtag atgacagaac aacaggcgac agttcaaaaa
      481 ggaaacggcg gacgaagcgg gaagcgatgg tttcttccct ttctgacgat tttcgcggtg
      541 ctcgcgtatc tctccttccg ggcttccttc tccctgatag gacctcttgc ctggtccgcg
      601 ctcatctcct tcacggtcta tccgctgtac atacgaatgc ggcggcgctt tttctcccgc
      661 cgttccgcga acctgcccgc ccttctggcc accgcgatca tcgtcttcgc cgtcctgatc
      721 ccctgcctgc tggggctttt cttcgccgcg cgcgaggggg tgcgcctgct cgacagcacg
      781 gaggagctgc tgacctcgat ggagtcctcg ggcgaggggg ccatcgccac gctgctgccg
      841 gagcacatcg tatcgaagat ccgtccgtgg atggagcggt acccgacgct tcgggcgtat
      901 gtgcgccaga cggtcgcgcg aagcgccgag agcgccgtcg atcttgtcgg cagcgcgatt
      961 ctttttctct actacttctc catcatcacc gtggccaact tcttcttcct cagggacggg
     1021 catctgatca tcggctttct gcgggagatc gtc
//
LOCUS       DAOTIL010001074         1053 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminobacteriaceae bacterium isolate AMR_MDS_3026
            NODE_171055_length_1053_cov_3.230461, whole genome shotgun
            sequence.
ACCESSION   DAOTIL010001074 DAOTIL010000000
VERSION     DAOTIL010001074.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868050
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminobacteriaceae bacterium (wastewater metagenome)
  ORGANISM  Aminobacteriaceae bacterium
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae.
REFERENCE   1  (bases 1 to 1053)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1053)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:19:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,224
            CDSs (total)                      :: 3,198
            Genes (coding)                    :: 3,184
            CDSs (with protein)               :: 3,184
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1053
                     /organism="Aminobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_171055_length_1053_cov_3.230461"
                     /isolate="AMR_MDS_3026"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:3034609"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..277
                     /locus_tag="P5201_15785"
     CDS             <1..277
                     /locus_tag="P5201_15785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015556458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="nitrilase-related carbon-nitrogen hydrolase"
                     /protein_id="HRW00062.1"
                     /translation="WPSVRADHWRTLLRARAIENQMYVVACNRCGTSGDTHFGGGSMI
                     LGPKGEIYFEGGGEEAGFATIDLDEVAETRNFLTVFNDRVPEIYSVK"
     gene            396..>1053
                     /locus_tag="P5201_15790"
     CDS             396..>1053
                     /locus_tag="P5201_15790"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRW00063.1"
                     /translation="MKLYGKILAPMLLLFFLGIATVSFTGYALSLRVLTEASQQKVLQ
                     AADIAEAMQRVGVVSSLIALGVVLVAGIVMLLVVRSIVKPLRDVVRVLVRRSELDFTV
                     NPADLWMIKRSKAKDEIAEMIQALILLRVKVEEMLRPLLTRSEAFSKSAQSLAELSEE
                     TVASMQEVKSSIETVFSLSEENAEAIRRTESGVGDVASSAEIAAASAQEGAKSASATS
                     E"
BASE COUNT          204 a          313 c          332 g          204 t
ORIGIN      
        1 gtggccttcc gtgagggcgg accactggcg gacgctgctc cgcgccaggg ccatcgaaaa
       61 ccagatgtac gtggtggcct gcaaccgatg cggaacctcc ggcgatacgc atttcggcgg
      121 cggctccatg atcctcggcc cgaagggaga gatctacttc gagggcggcg gcgaagaggc
      181 gggattcgcc accatcgatc tcgacgaagt cgccgaaacc aggaatttcc tcacggtctt
      241 caacgacagg gttcccgaaa tatactctgt gaaatagaaa aaacaccgtt ctccggcttg
      301 gtgcgcgtac cggtgcgttc gagaccgacg cgaccggaaa cggcacgatt gttgttgcgc
      361 ggattcacaa gtctttatca gggaggggtt tgtgtatgaa gttgtacgga aagattctgg
      421 cgccgatgct cctgctgttc ttcctgggaa ttgcgacggt ctcctttacg gggtacgcgc
      481 tttcgcttcg cgtgctgacc gaggcgtctc aacaaaaagt ccttcaggcc gccgacatcg
      541 ccgaagcaat gcaacgtgtg ggcgtcgtct cgtcgctgat cgcgctcgga gtggttctgg
      601 tcgccggaat cgtcatgctg ctcgtcgtcc ggtcgatcgt caaacccctg cgcgacgtcg
      661 tccgcgtgct ggtccgccgc agcgaactcg acttcacagt gaatcccgcc gacctctgga
      721 tgatcaagcg gtcgaaggcg aaggacgaga tcgcggagat gatccaggcg ctcatcctgc
      781 tgcgggtgaa ggtggaagag atgttgcgcc ccctcctcac ccgttcggag gccttctcga
      841 agagcgcgca atcccttgcg gagctctccg aggagacggt cgcctccatg caggaggtca
      901 aaagctcgat agagacagta ttctctctct cggaggagaa cgcggaagct atccgccgga
      961 ccgagtcggg cgtcggcgac gtcgcctcct cggccgaaat cgccgcggct tccgcccagg
     1021 agggtgcgaa aagcgcctcc gcgacatcgg aga
//
LOCUS       DAOTIL010001075         1053 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminobacteriaceae bacterium isolate AMR_MDS_3026
            NODE_171158_length_1053_cov_2.115230, whole genome shotgun
            sequence.
ACCESSION   DAOTIL010001075 DAOTIL010000000
VERSION     DAOTIL010001075.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868050
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminobacteriaceae bacterium (wastewater metagenome)
  ORGANISM  Aminobacteriaceae bacterium
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae.
REFERENCE   1  (bases 1 to 1053)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1053)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:19:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,224
            CDSs (total)                      :: 3,198
            Genes (coding)                    :: 3,184
            CDSs (with protein)               :: 3,184
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1053
                     /organism="Aminobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_171158_length_1053_cov_2.115230"
                     /isolate="AMR_MDS_3026"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:3034609"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..477
                     /gene="dctP"
                     /locus_tag="P5201_15795"
     CDS             <1..477
                     /gene="dctP"
                     /locus_tag="P5201_15795"
                     /inference="COORDINATES: protein motif:HMM:NF015445.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter substrate-binding protein DctP"
                     /protein_id="HRW00064.1"
                     /translation="VPETKTVMDTIAALGGNPVPMVFGEVYTALQQGIIDGQENPLSI
                     IWASRLYEVQKYLSLTGHVYSPVVIMISDAFYNSLPEDLQKIVVEAFEEVRPVARKMV
                     EDQETELVEQLREKGMEVNTVEPEAFQAKMGGVYDDFVKNVGKEAQEFIDRIKEVR"
     gene            554..1051
                     /locus_tag="P5201_15800"
     CDS             554..1051
                     /locus_tag="P5201_15800"
                     /inference="COORDINATES: protein motif:HMM:NF016200.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter small permease"
                     /protein_id="HRW00065.1"
                     /translation="MKKISALVSAAAAYIDKAATWLLFLLLFVMTGITFAQVVTRYVF
                     NFSITWSEELARFLFIWSIFAGIPSLIFRSGMTAFDLILARLQGKIAKCAVLLLSGAG
                     LLFFHLMAYGSFPLVQRQFAQQATSLPVPMGLVYIVIPVSGFTAMFMIVENLLRVLSE
                     RGEAA"
BASE COUNT          210 a          291 c          313 g          239 t
ORIGIN      
        1 gtccccgaga cgaagacagt catggacacc attgccgcgc tgggcggaaa ccccgtaccc
       61 atggtcttcg gcgaggtcta cacggcgctg cagcagggga tcatcgacgg ccaggagaat
      121 ccgctctcca tcatctgggc ctctcgtctc tacgaggtgc agaagtatct ctctcttacc
      181 ggacacgtgt actctcccgt ggtcatcatg atctccgacg ccttctacaa ctctctccct
      241 gaagatcttc agaagatcgt tgtggaggcc ttcgaggaag tcaggccggt cgccagaaaa
      301 atggtcgagg atcaggagac cgaactggtt gagcagctga gagagaaggg gatggaggtc
      361 aacactgtcg agcctgaggc attccaggcg aagatgggcg gcgtgtacga cgacttcgta
      421 aagaacgtcg ggaaagaggc gcaggagttc atcgaccgga tcaaggaagt ccggtagttt
      481 ttcgagctac tccggccctc aatgcgcgca gagggggccg gagtcttttt atagacccga
      541 aggagaaata cggatgaaaa aaatcagcgc tcttgtctcc gccgcggccg cgtacatcga
      601 caaggccgca acgtggcttc tttttttgct tctgttcgta atgacgggga tcacgtttgc
      661 gcaggtggtt acccggtacg tctttaactt ttcgatcacg tggagcgaag agctggcgcg
      721 ctttctcttc atttggagca tcttcgcggg catcccgtcg ctgatcttcc gttccgggat
      781 gaccgcattc gatctcattc tcgcacgtct tcagggaaag atcgccaagt gcgccgtgtt
      841 gctgctctca ggcgcagggc ttctcttttt ccacctgatg gcgtatggca gcttcccgct
      901 tgtgcagcgg cagttcgctc agcaggcgac ctctctcccc gtcccgatgg ggctcgtgta
      961 catcgtgatc ccggtaagcg gcttcacggc gatgttcatg atcgtcgaga accttctgcg
     1021 ggtgctctcg gaaagggggg aagccgcatg atg
//
LOCUS       DAOTIL010001076         1053 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminobacteriaceae bacterium isolate AMR_MDS_3026
            NODE_171232_length_1053_cov_1.318637, whole genome shotgun
            sequence.
ACCESSION   DAOTIL010001076 DAOTIL010000000
VERSION     DAOTIL010001076.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868050
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminobacteriaceae bacterium (wastewater metagenome)
  ORGANISM  Aminobacteriaceae bacterium
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae.
REFERENCE   1  (bases 1 to 1053)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1053)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:19:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,224
            CDSs (total)                      :: 3,198
            Genes (coding)                    :: 3,184
            CDSs (with protein)               :: 3,184
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1053
                     /organism="Aminobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_171232_length_1053_cov_1.318637"
                     /isolate="AMR_MDS_3026"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:3034609"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..710)
                     /locus_tag="P5201_15805"
     CDS             complement(<1..710)
                     /locus_tag="P5201_15805"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRW00066.1"
                     /translation="MAKYLADGGEGKTLSGFAGELPRYWASRLSMEECVALFLAPDQA
                     WRRDYARLKETGAPPGAEFMELLSKRLGWEAFTEFGLSAHIGRTLEKSGVAVASPDPA
                     LFGHSAALLAEALKNELPKMTPPEEREISFFLSGLIQRMKSKGAIHHPELEEYIRRGG
                     EGYLLSKERKGWMPSYGKRTRRPLFLSAAADGDFDRLSFSEPRNWYLRWKLKCFGENN
                     PLILSQGADFWRTVLGVLS"
     gene            804..953
                     /locus_tag="P5201_15810"
     CDS             804..953
                     /locus_tag="P5201_15810"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRW00067.1"
                     /translation="MSSFPRDAMVCEIMTLDRVVLRAPKMESPLDEPQKGQSLCENTL
                     LSPFS"
BASE COUNT          196 a          350 c          324 g          183 t
ORIGIN      
        1 gagagaacgc cgaggaccgt cctccagaaa tcggccccct gagaaagaat cagtggattg
       61 ttctccccga agcatttcag cttccaccgg aggtaccagt tcctgggctc cgaaaaggag
      121 aggcggtcga aatcgccgtc cgccgccgcg gagaggaaaa gaggccgccg cgtccgcttc
      181 ccgtaggagg gcatccaccc tttccgctct ttcgagagca gatacccctc tcctccgcgc
      241 cggatatact cctccagctc ggggtgatgg atcgccccct tcgacttcat cctctggatc
      301 agcccggaga gaaagaaaga gatctcccgc tcctccggcg gcgtcatctt cggcagttcg
      361 tttttcagcg cctccgcgag gagggcggcg gaatggccga acaacgccgg gtcgggcgag
      421 gcgactgcca cgcccgattt ctccagggtg cgccctatgt gggcgctcag gccgaactcg
      481 gtgaacgcct cccacccgag ccgcttcgac agcagctcca tgaactccgc gcccggcggc
      541 gcacccgtct ccttcaggcg ggcgtaatct cttcgccacg cctgatccgg ggcgagaaaa
      601 agcgcgacgc actcctccat ggacaggcgg gaagcccagt accggggcag ctccccggcg
      661 aaaccggaga gcgtcttccc ctccccgccg tcggcaaggt acttcgccat ggcggtccgc
      721 agcgtgaatc tgtaggtcct cgccccgaag aaccggcgtg atgggcggca tcctgcaccg
      781 agtcggagaa ggccagcatc ttcttgtcgt cgtttccccg cgacgccatg gtctgcgaga
      841 tcatgacgct cgacagggtc gtgctccgag cgccgaagat ggagagcccc cttgacgaac
      901 cgcagaaagg gcagtcgtta tgtgagaata ccctgttgtc gccgttctcg taggtcttct
      961 tgaactgtga gtcgggaacg aagcctggga gcaggggggc gtttccgcag aaggggaagt
     1021 cctctccgtc ccgcacgtcg gagaacgcca tgc
//
LOCUS       DAOTIL010001077         1051 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminobacteriaceae bacterium isolate AMR_MDS_3026
            NODE_171531_length_1051_cov_4.068273, whole genome shotgun
            sequence.
ACCESSION   DAOTIL010001077 DAOTIL010000000
VERSION     DAOTIL010001077.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868050
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminobacteriaceae bacterium (wastewater metagenome)
  ORGANISM  Aminobacteriaceae bacterium
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae.
REFERENCE   1  (bases 1 to 1051)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1051)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:19:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,224
            CDSs (total)                      :: 3,198
            Genes (coding)                    :: 3,184
            CDSs (with protein)               :: 3,184
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1051
                     /organism="Aminobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_171531_length_1051_cov_4.068273"
                     /isolate="AMR_MDS_3026"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:3034609"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>1051
                     /locus_tag="P5201_15815"
     CDS             <1..>1051
                     /locus_tag="P5201_15815"
                     /inference="COORDINATES: protein motif:HMM:NF018208.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromosome segregation protein SMC"
                     /protein_id="HRW00068.1"
                     /translation="CVRGLVLSPELHEAIDAERRRKDETTNTLASLSGELETLRSRLR
                     SRRSFLETCGADLAASEEALRTLEESHAAASRNLEQLEEREPELVEAHSEIFARCRTT
                     GGTLQSVRKEASALEAALEDLRSAEDSMYPEPVRFLTAASRLGKLSVPVKVAADAFTC
                     PQRVTSGLEAYLGGRQYWVLVKTLAEAGQCIEMLKERRAGRATFLPLERSRPRFPQSR
                     FSLPKSGVVGWAMDLVTVDREWESAVMHLLGDLLVVERYEDGSALVNKGASFPIVSLD
                     GEVFAPSGTVSGGRARSSAGAIERRSRILESEEKLKSLKRRISDLTKTLAEEEALERT
                     RAAEKDECVLGVREAK"
BASE COUNT          183 a          331 c          354 g          183 t
ORIGIN      
        1 tgtgttcgtg gtctcgtcct ttcgcctgag ctgcacgaag cgatcgacgc ggagcgcagg
       61 cgaaaggacg agaccacgaa cacactcgcc tctctctccg gcgagctgga gacgctccgt
      121 tcacgccttc gctcgcggcg ttccttcctg gagacgtgcg gcgctgacct tgccgcctcc
      181 gaagaggcct tgcggactct ggaggagtcg catgccgcag cctcccggaa tctcgaacag
      241 cttgaggaga gagaaccgga gctcgtagaa gcccacagcg agatttttgc ccgctgccgg
      301 acgaccggag gaacgcttca gtccgtccgc aaggaggcct ctgcgctcga agcggcgctc
      361 gaagacctgc ggagcgccga ggactccatg tacccggagc ccgtccgctt cctcaccgcg
      421 gcctcaaggc tcgggaagct ctccgtcccg gtgaaggtgg ccgcggatgc gttcacctgt
      481 ccgcagcgcg tgacatccgg tctggaagcg taccttggcg gccgccagta ctgggtgctc
      541 gtcaaaaccc tcgcggaagc cggtcagtgc atcgaaatgc tcaaggagcg gcgcgccgga
      601 cgagcgacgt tcctcccgct cgaacgaagc cgcccgcgct ttccgcagag caggttttcc
      661 ctgccgaaga gcggcgttgt cggctgggct atggatcttg tcacggtcga ccgggagtgg
      721 gagtccgccg tgatgcacct tctcggcgat ctcctggtgg tcgagcgcta cgaggacggc
      781 tccgctctcg tgaacaaggg ggcgtccttc ccgatagtct cgctcgacgg cgaggtgttc
      841 gccccgtcgg gaaccgtcag cggcggacgc gctcgttcat ctgcgggcgc gatcgaacgc
      901 agaagccgta ttcttgagtc ggaggagaag ctgaagagcc tgaagcggcg gatttccgac
      961 ctgacgaaga cgcttgccga agaggaggcg ctcgaacgta cccgcgccgc ggagaaggat
     1021 gaatgtgttc tcggtgtgcg ggaggcgaag a
//
LOCUS       DAOTIL010001078         1050 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminobacteriaceae bacterium isolate AMR_MDS_3026
            NODE_171837_length_1050_cov_2.943719, whole genome shotgun
            sequence.
ACCESSION   DAOTIL010001078 DAOTIL010000000
VERSION     DAOTIL010001078.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868050
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminobacteriaceae bacterium (wastewater metagenome)
  ORGANISM  Aminobacteriaceae bacterium
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae.
REFERENCE   1  (bases 1 to 1050)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1050)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:19:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,224
            CDSs (total)                      :: 3,198
            Genes (coding)                    :: 3,184
            CDSs (with protein)               :: 3,184
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1050
                     /organism="Aminobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_171837_length_1050_cov_2.943719"
                     /isolate="AMR_MDS_3026"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:3034609"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            301..>1050
                     /locus_tag="P5201_15820"
     CDS             301..>1050
                     /locus_tag="P5201_15820"
                     /inference="COORDINATES: protein motif:HMM:NF017828.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxyacyl-CoA dehydratase family protein"
                     /protein_id="HRW00069.1"
                     /translation="MATLRSSRVLLDHIADESPAAPVRVKEAKERGVPVVGTFCVFTP
                     WELVRAVGGISVSLCSTSEKPIAAAEAHLPRNLCPLIKASYGFALTDTCPYFHFCDIV
                     IGETTCDGKKKMYEYLNRLRPVHVMQLPQTTEGETARALWRAETERLKSVLESFYGRT
                     ITDDALREAIRLKNRERRVLNRFFAVARLDPSPLTGLEILAVSDYLKYSMASEEALCV
                     VEQLTAALEEERRRGKGSETAGRKRILITGCP"
BASE COUNT          213 a          335 c          313 g          189 t
ORIGIN      
        1 cgacgatggc cccgagacgg cgccttcaaa acgaattctt cgggagatcc ccgaatacga
       61 caaagcgact gccggcgttt ccgtagcggc gcatatcggg ttgccgaccc tgcgaaagaa
      121 gtgcagacac ttcgacgaat gggtcactca actggagagc ctggggaaag aatgagggag
      181 agtcatcgtt cccgttgtta aaaaaacctc tttcggttat aatttttatt ataataacgg
      241 caaatgcggc gccctatcat ctcctctcgc cgcctgcttc ctgaaagggg tttccgtttc
      301 atggcaacgc ttcgttcatc ccgtgtcctg ctcgaccata tcgccgacga atcgcccgcc
      361 gcgccggtgc gcgtgaagga ggcgaaggag cggggcgttc ccgtggtggg aactttctgc
      421 gtcttcaccc cctgggagct tgtccgcgcc gttgggggaa tctccgtctc gctgtgcagc
      481 accagcgaaa aaccgatcgc cgcagccgag gcgcacctgc cgcgcaacct ctgtccgctg
      541 atcaaggcga gctacggctt cgccctcacc gacacctgcc cgtacttcca cttctgcgac
      601 atcgtcatcg gcgagaccac ctgcgacggc aagaagaaga tgtacgagta cctgaaccgc
      661 ctccgccccg tccacgtgat gcagctcccc cagacgacgg agggggagac ggcccgcgcg
      721 ctctggcggg cggagacgga gcgcctgaaa agcgtcctcg aatcgttcta cggaaggacg
      781 atcaccgacg atgcgctccg cgaggcgatc cgcctcaaaa accgtgaacg gcgcgtcctc
      841 aaccgcttct tcgccgtcgc gcggctcgac ccgtccccgc tgacggggct cgaaatcctc
      901 gccgtctctg actatctgaa gtactccatg gcgtcggagg aggcgctctg cgtggtggag
      961 cagctgacgg cagcgctcga agaggagcgg cgacgaggaa agggaagcga aacggctgga
     1021 cgcaaacgta ttctgatcac cggctgcccg
//
LOCUS       DAOTIL010001079         1050 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminobacteriaceae bacterium isolate AMR_MDS_3026
            NODE_171842_length_1050_cov_2.856281, whole genome shotgun
            sequence.
ACCESSION   DAOTIL010001079 DAOTIL010000000
VERSION     DAOTIL010001079.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868050
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminobacteriaceae bacterium (wastewater metagenome)
  ORGANISM  Aminobacteriaceae bacterium
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae.
REFERENCE   1  (bases 1 to 1050)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1050)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:19:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,224
            CDSs (total)                      :: 3,198
            Genes (coding)                    :: 3,184
            CDSs (with protein)               :: 3,184
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1050
                     /organism="Aminobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_171842_length_1050_cov_2.856281"
                     /isolate="AMR_MDS_3026"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:3034609"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..184)
                     /locus_tag="P5201_15825"
     CDS             complement(<1..184)
                     /locus_tag="P5201_15825"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRW00070.1"
                     /translation="MRKHVKRTRAFAALLTLSAILWCGAAFAAGLSGLVFFSTQFAPV
                     EEQAKFREILKDGGFDY"
     gene            complement(318..>1050)
                     /locus_tag="P5201_15830"
     CDS             complement(318..>1050)
                     /locus_tag="P5201_15830"
                     /inference="COORDINATES: protein motif:HMM:NF012742.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HRW00071.1"
                     /translation="PREGEMELYQRKVSSGQVDGFVVVRTRRRDPRIAWLYKSQVPFV
                     AFGRTENGADFPFVDVDGVKGMRLIGEHLIGLGHRRIVFLSSPSELLFVEHRLDGLRQ
                     AMTTHGLDPDEMRILQGDLTQKSGYEATASLLDGGNPPSAIVACNDLMALGAMSALHE
                     RGISVGPDIAVTGFDDIPMAEHAHPPLTTIHQPIYEIGRMVCEKLVRLIAGEELASRS
                     EILEPRLIVRSSCGSLRKQPDQTGR"
BASE COUNT          183 a          324 c          336 g          207 t
ORIGIN      
        1 tgtaatcgaa gcccccgtcc ttcagaatct cgcgaaactt cgcctgctct tcgaccggcg
       61 cgaactgagt ggagaagaag accagcccgg agagtccggc ggcgaacgcg gcgccgcacc
      121 acaggattgc gctcagcgtg agcagagcgg cgaacgctcg tgttcttttc acgtgctttc
      181 tcatttcatt cctccttgta cacacatcct cccacgcctg aaaaacactc cctggaacga
      241 tcgggcaggc ggcgggagcc cgttgtctta ccgccgtatt gcagcgtgtc attctgtgga
      301 tgaaggtccg cgccgcatca cctccccgtt tggtccggct gcttccggag agagccgcag
      361 gaggagcgta cgatcagccg cggctccagt atctcgctgc gggaggccaa ttcctcgccg
      421 gcgatcagcc ggacgagctt ttcgcagacc attcgtccta tttcgtagat gggttgatga
      481 atcgtggtca gcggagggtg cgcgtgctcc gccatcggga tgtcgtcgaa acctgtgacg
      541 gcgatgtccg gaccgacgga gatcccgcgc tcgtggagcg cgctcatcgc gcccagagcc
      601 atcaggtcgt tgcatgctac gatcgcggag ggaggattcc cgccgtcgag cagcgaggcg
      661 gtcgcctcgt atccgctctt ctgggtgaga tctccctgga gtatccgcat ttcgtcggga
      721 tcgaggccgt gcgtggtcat cgcctggcgc agaccgtcga gccggtgctc gacaaagagc
      781 agttcggagg gggagctcag aaagacgatg cgccggtggc cgaggccgat gagatgttct
      841 ccgatcagac gcatcccctt gacgccgtcc acatcgacga aggggaaatc cgcgccgttt
      901 tcggttcgtc cgaaggcgac gaaggggact tgggatttgt agagccatgc gatacggggg
      961 tcgcggcgcc gggtgcggac gacgacgaag ccgtccacct ggccgctgct taccttgcgc
     1021 tggtagagct ccatctcccc ttctctcggc
//
LOCUS       DAOTIL010001080         1049 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminobacteriaceae bacterium isolate AMR_MDS_3026
            NODE_172085_length_1049_cov_3.468813, whole genome shotgun
            sequence.
ACCESSION   DAOTIL010001080 DAOTIL010000000
VERSION     DAOTIL010001080.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868050
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminobacteriaceae bacterium (wastewater metagenome)
  ORGANISM  Aminobacteriaceae bacterium
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae.
REFERENCE   1  (bases 1 to 1049)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1049)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:19:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,224
            CDSs (total)                      :: 3,198
            Genes (coding)                    :: 3,184
            CDSs (with protein)               :: 3,184
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1049
                     /organism="Aminobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_172085_length_1049_cov_3.468813"
                     /isolate="AMR_MDS_3026"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:3034609"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..956
                     /locus_tag="P5201_15835"
     CDS             <1..956
                     /locus_tag="P5201_15835"
                     /inference="COORDINATES: protein motif:HMM:NF024634.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HRW00072.1"
                     /translation="FTWTWGTLAWHEYDPWVSWMHLASGWEEMVASPWGYVVLFFVVI
                     GAGLFIERFWCRYLCPLGAALGILQKFSLTKVRRSKETCISCSKCNRSCPMGITPMAL
                     EKVTDADCIACGRCTESCPVEKTLQFSVAGKKVLSALAVGLLGVGLYFAAYGTAKITG
                     YWQTFASTSVEALKDPVDGVFGWMTVEQVAAQVHLPSAKILEIAGLPAESPTNVSIKQ
                     MEGVNDEELKEQLKAYFAANPAKAPEPKTVVPSNPDELKGSFTLAEVASGYGLELSAI
                     LEKAGWPADADPNVKMRDVANTIGRDSSDIRNAVKELLENR"
BASE COUNT          217 a          309 c          329 g          194 t
ORIGIN      
        1 actttacatg gacctgggga acgcttgcat ggcacgaata cgacccgtgg gtctcgtgga
       61 tgcacctcgc ctccggatgg gaggagatgg ttgcctctcc gtgggggtac gtcgttctct
      121 tctttgtcgt catcggcgcg gggctcttca tcgaacgttt ctggtgccgc tatctctgcc
      181 ctctgggcgc agccctcggc atcctgcaga agttcagttt gacgaaggtg cggcgcagca
      241 aagagacctg catctcctgc tccaagtgca accgctcgtg cccgatggga ataacgccta
      301 tggcgcttga aaaagtcacc gacgcggact gcatcgcctg cggccgctgc acggagagct
      361 gtccggtcga gaaaacgctt cagttctccg tcgccgggaa gaaagtgtta tcggcgctcg
      421 cggtcggact gctcggcgtg ggcctctact tcgccgccta cggcacggcg aagatcaccg
      481 gctactggca gacattcgcc tccacctcgg tcgaggcgct gaaggatcct gtggacggcg
      541 tcttcgggtg gatgaccgtc gaacaggtgg ctgcgcaggt acaccttccg tcggcaaaaa
      601 ttctggagat agccgggctt cccgccgagt cgcccacaaa cgtctcgatc aagcagatgg
      661 aaggcgtgaa cgacgaggag ctcaaggagc agctcaaggc ctacttcgcg gcaaacccgg
      721 ccaaggctcc tgaaccgaag acagtcgtgc cgtccaaccc ggacgaactg aagggaagct
      781 tcacgctcgc ggaggtggcg tcggggtacg gtctggaact ctccgccatc ctcgaaaaag
      841 cagggtggcc tgcggacgcc gatccgaacg tcaagatgcg cgacgtggcg aatacgatcg
      901 ggcgcgactc ctcggacatc cggaacgcgg tgaaggagct gctggagaat cgctgatcga
      961 acacgggagg acggaaacgg aataagaaag cgggagaatc tctcccccgc tttttcgtac
     1021 ctatgagctt acaggtcgaa tacagcaac
//
LOCUS       DAOTIL010001081         1049 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminobacteriaceae bacterium isolate AMR_MDS_3026
            NODE_172089_length_1049_cov_3.285714, whole genome shotgun
            sequence.
ACCESSION   DAOTIL010001081 DAOTIL010000000
VERSION     DAOTIL010001081.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868050
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminobacteriaceae bacterium (wastewater metagenome)
  ORGANISM  Aminobacteriaceae bacterium
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae.
REFERENCE   1  (bases 1 to 1049)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1049)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:19:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,224
            CDSs (total)                      :: 3,198
            Genes (coding)                    :: 3,184
            CDSs (with protein)               :: 3,184
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1049
                     /organism="Aminobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_172089_length_1049_cov_3.285714"
                     /isolate="AMR_MDS_3026"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:3034609"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..253)
                     /locus_tag="P5201_15840"
     CDS             complement(<1..253)
                     /locus_tag="P5201_15840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008521872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HRW00073.1"
                     /translation="MDSSQLQALLRSKVETMPNKARRVVEYLLANAREAAFLSIGEVA
                     EKLNVSKAQLVRVSRMVGFDGYAALKEALQDTVLEQVNPA"
     gene            complement(376..>1049)
                     /locus_tag="P5201_15845"
     CDS             complement(376..>1049)
                     /locus_tag="P5201_15845"
                     /inference="COORDINATES: protein motif:HMM:NF027524.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRW00074.1"
                     /translation="ILDSLASYPVSMIELGISSLDDDVLNACNRGYTGEFAIAAMRLI
                     LERGFSLGAQLMIGLPGQTEESSLNDLRRIAALRGQREPFPVTLRIYPCLVLDKTPLA
                     ELMRKGAYAPLSLEEGILRAGRLHFEAERLGFAVQRIGLHETESLAKSVLAGPHHPAM
                     GEMARSVAFVASLLESSSAGPWEVERRQISLLTGHDKFGLRYMAQKTGFRLQFIEKQI
                     RYIRS"
BASE COUNT          233 a          284 c          257 g          275 t
ORIGIN      
        1 ccgcaggatt cacctgttcg agcaccgtat cctggagggc ttccttcaat gccgcgtagc
       61 cgtcgaaacc aaccatgcgc gacacgcgta ccagttgtgc tttcgagacg ttcagctttt
      121 ccgcaacttc gccgattgag agaaacgccg cctccctggc gtttgccaga aggtattcga
      181 caacccttct cgccttgttt ggcattgttt ccactttgct tcgcaacagt gcctggagtt
      241 gcgaactatc catgaaagac acctccgaaa ctgtttagta cttcgaattt tctcttgaga
      301 cttaagttta ttctgttgga gatgcgccgt caatcgttct ccgctcattt tcttgttttt
      361 tcgatagaaa attatttaag atcgtatata gcgtatttgt ttttctatga actgcaagcg
      421 aaagcctgtt ttttgcgcca tgtagcgcag tccgaattta tcgtgacctg tcagaagcga
      481 tatctgcctt ctctctactt cccatggtcc cgccgaagaa ctttccagaa gggaggcgac
      541 gaaggcgacc gaacgcgcca tttcccccat cgccggatga tgcggccctg cgaggacgct
      601 tttggcaagg ctttctgttt catgaaggcc tattcgttga acggcgaatc caagacgttc
      661 ggcctcgaag tgcaatctgc ccgcgcgaag aattccttct tcaagcgaga gcggagcgta
      721 ggcgcccttt ctcatgagtt cggcgagggg cgtcttgtct aaaacaagac aaggatagat
      781 tctaagcgtt actggaaagg gttctctttg cccgcggagc gcggcaattc gtcggagatc
      841 gttcagagaa ctttcctctg tttgccccgg aagaccgatc atcaactgcg ccccgagaga
      901 aaaccctctt tccaaaataa gccgcatcgc tgcaatcgcg aactctcctg tgtacccccg
      961 gttacatgcg ttcagcacat cgtcgtcgag cgaggagatc ccgagttcga tcattgaaac
     1021 ggggtaggaa gcaagactgt cgagaatac
//
LOCUS       DAOTIL010001082         1048 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminobacteriaceae bacterium isolate AMR_MDS_3026
            NODE_172329_length_1048_cov_3.791541, whole genome shotgun
            sequence.
ACCESSION   DAOTIL010001082 DAOTIL010000000
VERSION     DAOTIL010001082.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868050
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminobacteriaceae bacterium (wastewater metagenome)
  ORGANISM  Aminobacteriaceae bacterium
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae.
REFERENCE   1  (bases 1 to 1048)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1048)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:19:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,224
            CDSs (total)                      :: 3,198
            Genes (coding)                    :: 3,184
            CDSs (with protein)               :: 3,184
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1048
                     /organism="Aminobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_172329_length_1048_cov_3.791541"
                     /isolate="AMR_MDS_3026"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:3034609"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1042)
                     /locus_tag="P5201_15850"
     CDS             complement(<1..1042)
                     /locus_tag="P5201_15850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005661226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dicarboxylate/amino acid:cation symporter"
                     /protein_id="HRW00075.1"
                     /translation="MLGIVAGAVLGEKVQIVEPVGSIFLTLLKMLIVPLVFSSLVVGA
                     ASIGDPRDLGRIGIKTIAFYLVTTAVTITIGLLLGNFLQPGVGLAIGEGAAFKAPEAP
                     SIKNVIMGLFPANPLKAAVDGNMLQIIVFALFLGVSAVLAGEKGKPFINFFDSLAETM
                     YKLTGIVMKIAPYGVFALIAVTVSKYGMSVLAPFAKVILAVYVGCALQVILVYSGLIT
                     VFVKKSPFWFLKGIREAGLTAFVTRSSSATLPITMRNTQENLGVSEKVSSFVLPLGAT
                     INMDGTALYQGVCALFVAQAYGLTLGMGAQLSIILTATLASIGTAGVPGAGLIMLTLV
                     LTAVGLPLEGAAL"
BASE COUNT          248 a          275 c          329 g          196 t
ORIGIN      
        1 cgagcgccgc gccttcaagg ggaaggccga ccgcagtaag gacaagtgtg agcatgataa
       61 gccccgctcc ggggacgcct gccgttccga tcgaagccag ggtcgcagtg aggatgatgc
      121 ttaactgcgc tcccattccg agcgtaagcc cgtaggcctg cgcgacgaag agggcgcaga
      181 caccctggta gagtgcggtt ccgtccatgt taatcgtcgc tccgagcggg aggacgaagg
      241 aagaaacctt ttcggagacg ccgagattct cctgggtgtt tctcatggtg atcggaagcg
      301 tcgcggagct ggagcgagtg acgaaggccg tcagtccggc ttcacgaatc cccttcaaga
      361 accagaaggg agacttcttg acgaataccg tgatcaggcc ggagtacaca aggatgacct
      421 ggagcgcgca cccgacgtag accgcaagaa tgaccttggc gaacggagcg agaaccgaca
      481 tcccgtactt ggaaacggtc acagcgatga gggcgaagac gccgtagggg gcgatcttca
      541 tgacgatgcc ggttagtttg tacattgttt cggccagtga atcaaagaaa ttgatgaacg
      601 gtttgccctt ttctccggcg aggactgcgg aaactcccag gaagagagca aagacgatga
      661 tctgcagcat gttgccgtca acagcggcct tcagcggatt cgcggggaag agacccatga
      721 taacgttctt gatgctcgga gcttcgggcg ccttgaatgc cgcgccttct cctattgcaa
      781 ggccgactcc gggctggagg aagttgccga gaagaagccc gatggtgatc gtgaccgcag
      841 tggtgacaag atagaatgcg atggttttga tgcctattct gccgagatct cgaggatcgc
      901 ctatcgaagc cgccccgacg acaaggctgg agaagacaag gggaacgatg agcatcttga
      961 gcagtgtgag gaagatgctt cctaccggct cgactatctg gaccttttcg ccaagcaccg
     1021 cgcctgctac gattccgaga atgaaacc
//
LOCUS       DAOTIL010001083         1045 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminobacteriaceae bacterium isolate AMR_MDS_3026
            NODE_173175_length_1045_cov_2.871717, whole genome shotgun
            sequence.
ACCESSION   DAOTIL010001083 DAOTIL010000000
VERSION     DAOTIL010001083.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868050
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminobacteriaceae bacterium (wastewater metagenome)
  ORGANISM  Aminobacteriaceae bacterium
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae.
REFERENCE   1  (bases 1 to 1045)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1045)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:19:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,224
            CDSs (total)                      :: 3,198
            Genes (coding)                    :: 3,184
            CDSs (with protein)               :: 3,184
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1045
                     /organism="Aminobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_173175_length_1045_cov_2.871717"
                     /isolate="AMR_MDS_3026"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:3034609"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>1045
                     /locus_tag="P5201_15855"
     CDS             <1..>1045
                     /locus_tag="P5201_15855"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HRW00076.1"
                     /translation="GELFWENVAISPILDENGAIAHFIAIKQDITERKKLIESLVRAK
                     EKAEESDRLKTAFLQNVSHEIRTPMNGILGFLHLLGQPALDDDERSEYIRIVNQSGQR
                     LLSTINDIIEASRIEAGKTTVQMDDVDVEQTMAFHFAFFRPRAEEKGLFLTLSEHLRG
                     EEALLRTDKHKLDSILNVLLGNALKFTCSGGVEFGNRLEGSELLFFVKDSGEGIPEDR
                     LEAVFDSFVQADLGVARSHEGAGLGLTIAKANVEALGGKIDVRSEVGRGSTFTFSIPY
                     LPLTPGAVISKSGAGAHSAEADQGGSLILIAEDDSTSFRLLEAMLKKAGYSALRAVDG
                     EEALQAVRENPDVA"
BASE COUNT          239 a          272 c          332 g          202 t
ORIGIN      
        1 ggagaactct tctgggaaaa cgtcgcgatt tcgcccattc tggatgaaaa cggcgctatt
       61 gcacatttca tcgcgatcaa acaggatatc acagagagga agaagctgat cgagtccctt
      121 gtacgcgcca aggaaaaggc cgaagagagc gaccggctga agacggcgtt cctccagaac
      181 gtatcgcacg agatacggac gccgatgaac ggaattctcg ggtttcttca cctgctcggg
      241 cagccggctt tggacgacga cgagcgaagc gagtacatcc ggatcgtcaa ccagagcgga
      301 cagaggctcc tgagcacgat caacgatatt atagaagcgt ccagaatcga agcgggaaag
      361 acgacggtgc agatggacga tgtcgacgtt gaacagacga tggcgttcca tttcgctttc
      421 ttccggcccc gggcggagga gaaggggctc tttttgacgc tttcggagca tcttcgggga
      481 gaagaggcgt tgctccgcac cgacaaacac aagctggaca gcatcttgaa cgttctgttg
      541 ggcaacgcgc tcaaattcac ctgcagcgga ggcgtcgaat tcgggaatcg cctggaaggc
      601 tccgaactgc tctttttcgt gaaggacagc ggagagggaa tccccgagga tcgtctcgaa
      661 gccgtcttcg acagctttgt gcaggcggat ctgggggtgg cgcgttcgca tgaaggcgcc
      721 ggattgggac tgaccatagc aaaggccaac gtcgaagccc tcggcgggaa aatcgatgta
      781 cgttccgagg tcggcagagg gagcacgttc actttttcga ttccgtatct gccgctcacg
      841 ccgggcgccg tgatttcaaa gagcggcgct ggagcccatt ccgcagaggc ggatcagggc
      901 ggttctctta tcctgattgc cgaggacgac tcgaccagtt tccgtctttt ggaggcgatg
      961 ctgaagaagg cggggtattc ggcgcttcgc gccgtcgacg gggaagaagc gctgcaggct
     1021 gtacgcgaaa acccggacgt agctc
//
LOCUS       DAOTIL010001084         1044 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminobacteriaceae bacterium isolate AMR_MDS_3026
            NODE_173381_length_1044_cov_4.102123, whole genome shotgun
            sequence.
ACCESSION   DAOTIL010001084 DAOTIL010000000
VERSION     DAOTIL010001084.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868050
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminobacteriaceae bacterium (wastewater metagenome)
  ORGANISM  Aminobacteriaceae bacterium
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae.
REFERENCE   1  (bases 1 to 1044)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1044)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:19:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,224
            CDSs (total)                      :: 3,198
            Genes (coding)                    :: 3,184
            CDSs (with protein)               :: 3,184
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1044
                     /organism="Aminobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_173381_length_1044_cov_4.102123"
                     /isolate="AMR_MDS_3026"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:3034609"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..574
                     /locus_tag="P5201_15860"
     CDS             <1..574
                     /locus_tag="P5201_15860"
                     /inference="COORDINATES: protein motif:HMM:NF014874.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="cyanophycin synthetase"
                     /protein_id="HRW00077.1"
                     /translation="DVVYKGKTFHASSPLFGRHSALAAGFGIALSLYLGASIEQALQS
                     IESLQPPCGRGRFFLLTSGAMLLDDSYNANPASMKASLSESLRIPVQRRIAVLGEMKE
                     LGKSALDYHRELLPFFTKFSKVFIMGTLWRTLFQNTPLPDNTVFFDNPLSLGTEIKKE
                     LGEGDLIVVKGSHGNRLDFLVDFLVEEGVE"
     gene            571..>1044
                     /locus_tag="P5201_15865"
     CDS             571..>1044
                     /locus_tag="P5201_15865"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-N-acetylmuramoyl-pentapeptide-
                     transferase"
                     /protein_id="HRW00078.1"
                     /translation="MIYALSWAFLFFLQIFCSKIWIARLHQWRVVQIQKSYGLQSHIE
                     TKSSTPSMGGVVFLLIGIASLLPEAVFSETRLSNAALFWILPLGSAAIGFIDDWIKLT
                     RKSSEGFPSLYKLAAQILLTVPWSCWIVFSQGLSLWPGVVVPALLAVPLLTIVSIG"
BASE COUNT          223 a          262 c          251 g          308 t
ORIGIN      
        1 cgacgtagtc tacaagggaa aaactttcca cgcatcatct cccctttttg gacgacattc
       61 ggcgttggcg gcgggattcg gaatcgcatt gtcactttat ttaggggcgt ctattgaaca
      121 agcccttcag tctattgaat ctctccagcc cccctgcgga aggggccggt tttttcttct
      181 gacttcggga gctatgcttc ttgacgactc ctacaacgca aatccggctt cgatgaaggc
      241 gtcgctctcc gaatcgcttc gtattccggt gcagcgtcgt atcgccgtac ttggtgaaat
      301 gaaggagctt ggaaaaagtg ctctggacta tcatcgagaa ctgcttccgt tttttaccaa
      361 attttccaag gtgttcatta tgggtacgct ctggcggact ctgttccaaa atacaccgct
      421 tcccgataat accgttttct ttgataatcc gctctcgctc ggaaccgaga taaagaaaga
      481 acttggcgaa ggagatctca ttgtagtcaa aggatcgcat ggtaacagac tggatttttt
      541 ggtcgatttc cttgtcgagg agggagtgga atgatttacg ctctttcctg ggctttcctc
      601 ttcttcttgc agatattctg ttctaagatc tggatcgcca ggcttcatca atggcgcgtt
      661 gtgcagattc aaaaaagcta cgggttgcaa agccacatcg aaacgaaatc atcgacgcca
      721 tccatgggtg gagttgtgtt tctcctcata ggaattgcga gtcttctgcc ggaagcagtt
      781 ttttctgaga cgcgcttatc taacgcggca ctcttctgga ttctgccttt gggatccgca
      841 gctatcggtt ttattgacga ttggatcaaa cttaccagga aatccagcga aggcttccct
      901 agcctctata aacttgccgc acagatactg cttacagtac cgtggtcgtg ttggattgtc
      961 ttttcacaag gcctctctct ctggccgggc gtcgttgttc ctgctctcct cgccgtcccg
     1021 cttctgacga tagtatcaat aggg
//
LOCUS       DAOTIL010001085         1044 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminobacteriaceae bacterium isolate AMR_MDS_3026
            NODE_173382_length_1044_cov_4.092012, whole genome shotgun
            sequence.
ACCESSION   DAOTIL010001085 DAOTIL010000000
VERSION     DAOTIL010001085.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868050
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminobacteriaceae bacterium (wastewater metagenome)
  ORGANISM  Aminobacteriaceae bacterium
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae.
REFERENCE   1  (bases 1 to 1044)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1044)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:19:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,224
            CDSs (total)                      :: 3,198
            Genes (coding)                    :: 3,184
            CDSs (with protein)               :: 3,184
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1044
                     /organism="Aminobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_173382_length_1044_cov_4.092012"
                     /isolate="AMR_MDS_3026"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:3034609"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            257..727
                     /locus_tag="P5201_15870"
     CDS             257..727
                     /locus_tag="P5201_15870"
                     /inference="COORDINATES: protein motif:HMM:NF013629.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fur family transcriptional regulator"
                     /protein_id="HRW00079.1"
                     /translation="MRSVEQIAEQLRKKGLKLTPQRRLIIDILLHDRTHPTVEEVYRK
                     VLETMPEVSRTTVYNTIREMLSLGEVAEVQDLSDGGIRYDTTGEPHHHFFCVRCRALV
                     DVNLDLPPMAESSTELSDCVIIKQQLTLYGLCPACRALQSQEEALKEKEAVAGR"
     gene            complement(796..>1044)
                     /locus_tag="P5201_15875"
     CDS             complement(796..>1044)
                     /locus_tag="P5201_15875"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRW00080.1"
                     /translation="ATMQEQSASAEEMTAGMDHVARSGAEIAEQVESINRSMDEQGRM
                     TESIASTAGDLVRLSEEMQRSVGRFKVAAEQSGLVVKK"
BASE COUNT          219 a          297 c          299 g          229 t
ORIGIN      
        1 cgatcgtatg ggaatggacg cgccggggtt gaaacaggcg ttcgccctct acgaagagct
       61 tgcggccaag gggtgcgaaa acgacggaac ccaggcgctc ttcaaactct atgaataaga
      121 tctcccggtt gtaaggcagg agatattctc cgacggcaag gtccttcgaa gggccttgtt
      181 tatttgtgga ttgacgcaaa aattgttttt gtttataatg gttacaagtt gcatcacgag
      241 aaaggaagtg acgctcatgc gctcagtcga acagatcgcc gaacagcttc ggaaaaaggg
      301 gctgaagctc acgccccagc ggcgtcttat tattgatatt ctccttcacg acaggacgca
      361 tcctactgtc gaggaggtgt accgcaaggt cttggagacg atgcccgaag tctccagaac
      421 gacggtgtac aatacgatcc gcgagatgct cagcctgggc gaggtcgcgg aggtacagga
      481 tttgagcgac ggcggaattc ggtacgatac cacgggcgag ccccatcacc acttcttctg
      541 cgttcgctgc agggcgctcg tcgacgtgaa tcttgatctt cctccgatgg cggagtcttc
      601 cacggagctt tccgactgcg tcattatcaa gcagcagctg acgctgtacg ggttgtgtcc
      661 ggcgtgcaga gcgttgcaaa gtcaagaaga ggcgcttaaa gagaaggaag cggtagccgg
      721 cagataggcg cacactaaaa aaagaagccc ccctgaattc cgggggcttc tttcgtggta
      781 tttttagggt gcagtctatt tctttacgac gagtccgctt tgttccgcgg cgaccttgaa
      841 gcgccccacg gagcgctgca tctcctccga caggcggaca aggtcccccg ccgtcgacgc
      901 gatgctctcc gtcatacgcc cctgctcgtc catcgagcgg ttgatgctct ccacctgctc
      961 ggcgatctcc gcgcccgatc gcgccacgtg gtccattccc gcggtcatct cctccgcgct
     1021 cgccgactgc tcctgcatcg tcgc
//
LOCUS       DAOTIL010001086         1044 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminobacteriaceae bacterium isolate AMR_MDS_3026
            NODE_173623_length_1044_cov_1.339737, whole genome shotgun
            sequence.
ACCESSION   DAOTIL010001086 DAOTIL010000000
VERSION     DAOTIL010001086.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868050
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminobacteriaceae bacterium (wastewater metagenome)
  ORGANISM  Aminobacteriaceae bacterium
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae.
REFERENCE   1  (bases 1 to 1044)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1044)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:19:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,224
            CDSs (total)                      :: 3,198
            Genes (coding)                    :: 3,184
            CDSs (with protein)               :: 3,184
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1044
                     /organism="Aminobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_173623_length_1044_cov_1.339737"
                     /isolate="AMR_MDS_3026"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:3034609"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(143..>1044)
                     /locus_tag="P5201_15880"
     CDS             complement(143..>1044)
                     /locus_tag="P5201_15880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005659950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter substrate
                     binding protein"
                     /protein_id="HRW00081.1"
                     /translation="YIMWGAGGAMDNVTRAIVPIAQEHLGKTIILQNRTGATGAIATT
                     FVANQPADGYSILFGAENPNLYKVTGLSQIDYDQFDPVFLMMANVGVVLVGKDSPYTT
                     YKELIEAAASGKTITMGSTGPGGLPFVATTMIEKIHDLKFNKMQFDGEGPCITALMGG
                     HIDAVAVGLLAAANFIKSDSVRGLAVISKERIDSIPAVPAITEAYEEYVPYLPWGAFF
                     GAFVRKGTPAEALEALQSAFQKGYEDPRFDEFAKSTGGVKLGLTGEKAREYIQQNKSV
                     SAWLLHDAGGAKHSPEEFGIPRP"
BASE COUNT          198 a          323 c          303 g          220 t
ORIGIN      
        1 ggcgacgtct tcagcaccgt aagctgcgcc agggaaaatt gaggctgcat aatcctactc
       61 tcctcctccg aatgtaaaaa cgttctcgaa cgacggcagt cgtcataggg agcgcccctt
      121 gccggggcgc tccaccttct cgttacggcc tgggaatacc gaattcctcc ggagagtgct
      181 tcgctccgcc tgcatcgtgc agaagccacg cgctgaccga cttgttctgc tgaatgtact
      241 cgcgggcctt ctcacccgtc agtccgagtt tcacgccgcc ggtactcttc gcgaattcat
      301 cgaagcgcgg gtcttcatac cctttctgga atgcggactg aagcgcttca agggcttctg
      361 cgggagttcc cttgcggaca aacgcgccga aaaacgctcc ccacgggagg tagggtacgt
      421 actcttcata cgcctcggta atcgcgggca ctgccggaat gctgtcgata cgctccttgg
      481 agatcacggc aagcccgcgc accgaatcgg acttgatgaa gttcgccgcc gcaagcagtc
      541 caaccgccac agcgtcgata tgtccgccca tgagcgccgt gatgcagggg ccttcgccgt
      601 cgaactgcat cttgttgaac ttcagatcgt gaatcttctc gatcatcgtc gtggccacga
      661 atggcaaccc gcccggtccc gtggagccca tcgtgatcgt cttcccggaa gcggcggcct
      721 cgataagctc cttgtaggtc gtgtaggggg aatccttccc gacgagaacc acgccgacgt
      781 ttgccatcat cagaaagacg gggtcgaact gatcgtagtc gatctgggag agaccggtta
      841 ccttgtagag attcggattc tccgcgccga agaggatact gtacccgtcc gcaggctggt
      901 tcgccacgaa ggtggtcgcg atggcgccgg tcgcgccggt ccggttttgc agaataatcg
      961 tctttcccag atgctcctgg gcgatgggga cgatagcgcg ggtgacgttg tccatggcgc
     1021 cgccggcgcc ccacatgatg tacc
//
LOCUS       DAOTIL010001087         1044 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminobacteriaceae bacterium isolate AMR_MDS_3026
            NODE_173634_length_1044_cov_1.182002, whole genome shotgun
            sequence.
ACCESSION   DAOTIL010001087 DAOTIL010000000
VERSION     DAOTIL010001087.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868050
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminobacteriaceae bacterium (wastewater metagenome)
  ORGANISM  Aminobacteriaceae bacterium
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae.
REFERENCE   1  (bases 1 to 1044)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1044)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:19:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,224
            CDSs (total)                      :: 3,198
            Genes (coding)                    :: 3,184
            CDSs (with protein)               :: 3,184
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1044
                     /organism="Aminobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_173634_length_1044_cov_1.182002"
                     /isolate="AMR_MDS_3026"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:3034609"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>1044
                     /locus_tag="P5201_15885"
     CDS             <1..>1044
                     /locus_tag="P5201_15885"
                     /inference="COORDINATES: protein motif:HMM:NF021570.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF2298 domain-containing protein"
                     /protein_id="HRW00082.1"
                     /translation="LLLPLTLRLFAGFHDGGYLFARVLGLLLSAWLAWLASSLGLAPF
                     GRTTVAASLLLGGALNYALRSSRSAVRDFFRRKARTVVAEEYLFLLAFLAWAFVRSLK
                     PDIDGLEKFMNLGFVNAVLRAEWMPPVDMWMAGESVNYYYFGHVATAFLCLLTRIPPE
                     IAYNLMIATLFALALSLSYSVVSCLLLKIDPRGAKKAVAGGLLAALLLAAGGNLQPFV
                     YGVIRPALQRAGVLEGEPASYWYPQARSFIGHHPPTGDKGIHEFPFYSFVISDLHGHV
                     LDVPASLTATGLGAALLLSQSSASGTSRIRVPYREAVAGVLLGATWMTNSWNYPIYFL
                     VLVGAALIFALQR"
BASE COUNT          134 a          394 c          309 g          207 t
ORIGIN      
        1 cctcttgctt ccgctcacgc tgcgcctctt cgcgggcttc cacgacggag ggtatctctt
       61 cgcgcgcgtg ctcggcctcc tgctgagcgc atggctcgcg tggctcgcct cctcgctcgg
      121 tctcgccccc ttcggccgga cgaccgtcgc cgcgtcgctc ctgcttgggg gcgcgctcaa
      181 ctacgccctc cggtcctccc gcagcgctgt gcgcgacttc ttccggcgga aagcgcgcac
      241 ggtcgtcgcg gaggagtacc tctttctgct cgccttcctc gcgtgggcct ttgtgcggag
      301 cctgaagccc gacatcgacg gtctcgaaaa attcatgaac ctcggtttcg tcaacgccgt
      361 tctccgggcc gagtggatgc cccccgtcga tatgtggatg gcgggggaga gcgtcaacta
      421 ctactacttc gggcacgtcg ccacagcctt cctctgcctg ctgacgcgca ttccgccgga
      481 gatcgcgtac aacctgatga tcgcgacgct cttcgcgctc gccctctccc tctcgtactc
      541 cgtcgtttcc tgccttcttt tgaaaatcga cccgaggggc gcgaagaaag ctgtcgccgg
      601 agggctcctc gccgcgctgc tgctcgccgc cggggggaac ctccagcctt tcgtctacgg
      661 ggtgatccgc cccgcgctcc agagggcggg cgtcctcgaa ggcgagccgg cgagctactg
      721 gtacccgcag gcgcgcagct tcatcggcca ccacccgccg accggagaca aggggatcca
      781 cgagttcccc ttctactcct tcgtcatctc cgacctccac ggacacgtcc tcgacgttcc
      841 cgcctcgctc accgccaccg gcctgggcgc cgcgctgctg ctgtcgcaaa gctccgcctc
      901 cgggacgtcg cgcatccgcg ttccgtaccg ggaagccgtc gcgggcgtgc tgctcggcgc
      961 gacgtggatg acgaacagct ggaactaccc gatctacttc ctcgtgctcg tgggcgcggc
     1021 gctcatcttc gcgctacaac ggca
//
LOCUS       DAOTIL010001088         1042 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminobacteriaceae bacterium isolate AMR_MDS_3026
            NODE_173951_length_1042_cov_3.163121, whole genome shotgun
            sequence.
ACCESSION   DAOTIL010001088 DAOTIL010000000
VERSION     DAOTIL010001088.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868050
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminobacteriaceae bacterium (wastewater metagenome)
  ORGANISM  Aminobacteriaceae bacterium
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae.
REFERENCE   1  (bases 1 to 1042)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1042)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:19:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,224
            CDSs (total)                      :: 3,198
            Genes (coding)                    :: 3,184
            CDSs (with protein)               :: 3,184
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1042
                     /organism="Aminobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_173951_length_1042_cov_3.163121"
                     /isolate="AMR_MDS_3026"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:3034609"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..583
                     /locus_tag="P5201_15890"
     CDS             <1..583
                     /locus_tag="P5201_15890"
                     /inference="COORDINATES: protein motif:HMM:NF025925.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ASKHA domain-containing protein"
                     /protein_id="HRW00083.1"
                     /translation="TTYCRTVNNKPAIGLCGSGLLDAVALLLREGVLNEDGAMEGRTT
                     SSFARRIRGEGSELRCVLCESKTSEVALYQCDVREFQLAKGAVQAGIELLLSHWGVAW
                     EEIGECILAGAFGNAIRHESLLETGVLRGLLRNRIRSAGNGAGMGIVRMLGNGESGRR
                     RVHRIAETAEHLRLESVPEFQSRFLEAMILAPR"
     gene            complement(607..>1042)
                     /locus_tag="P5201_15895"
     CDS             complement(607..>1042)
                     /locus_tag="P5201_15895"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRW00084.1"
                     /translation="AVETTCHFDWDECADIVGLLDFVFADIKHMDGGVHERLTGIGNE
                     RILSNMKRLGATGADAVVRVPLLSGVNDDEQNVARTARFVREHFRRPKMELLPYHDFG
                     RAKYRMLGMESFWTEFETPSKERVAFLEALIRSEGVEPVSYR"
BASE COUNT          184 a          317 c          346 g          195 t
ORIGIN      
        1 gacgacgtac tgccgcacgg tgaacaacaa gcctgccatc ggcctctgcg gcagcggact
       61 tctcgacgcc gtcgcgctgc ttctgcggga gggtgttctc aacgaggatg gcgccatgga
      121 gggtcggaca acgtcgtcct ttgcccgccg tatccgcggg gaagggtcgg agctgcgctg
      181 cgtcctctgt gaatcgaaga caagcgaagt ggcgctgtac cagtgcgatg tccgagaatt
      241 tcagcttgcg aagggggccg ttcaggcagg aatcgaactg ctgctgtcgc actggggcgt
      301 cgcctgggag gagatcggcg agtgtatcct cgcgggcgct ttcggcaacg cgatcaggca
      361 cgaatcgctg ctggagacgg gcgtgctgcg cggccttctc cgcaacagaa tacgctccgc
      421 aggaaacggc gcgggcatgg ggattgtccg catgctcggt aacggcgaga gcggccgcag
      481 gcgggtgcat cggatagcgg agacggcgga acatcttcgc ctcgaatcag ttccggagtt
      541 ccagtcccgt tttctggagg ccatgattct cgcgccccgc tgacttgggg tgcgaggcgt
      601 gcagccctac ctgtagctga ccggttccac accttcggag cggatgagcg cttccaggaa
      661 tgcgaccctc tccttcgaag gggtctcgaa ctccgtccag aacgactcca tgccgagcat
      721 cctgtacttc gcacgtccaa aatcgtgata cggaagcagc tccatcttcg gcctacgaaa
      781 gtgttcgcgg acgaagcgcg ccgtccgcgc gacgttctgc tcgtcgtcgt tgacgccgga
      841 aagcagcggc acgcggacga cggcgtcggc tccggttgct ccaagccgct tcatattcga
      901 gaggatgcgc tcgttgccga ttccggtcag gcgctcgtgg acgccgccgt ccatgtgctt
      961 gatgtcggcg aagacgaaat cgagcagccc aacgatgtcg gcgcactcgt cccagtcgaa
     1021 gtggcaggtc gtctcgacgg cc
//
LOCUS       DAOTIL010001089         1040 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminobacteriaceae bacterium isolate AMR_MDS_3026
            NODE_174577_length_1040_cov_2.195939, whole genome shotgun
            sequence.
ACCESSION   DAOTIL010001089 DAOTIL010000000
VERSION     DAOTIL010001089.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868050
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminobacteriaceae bacterium (wastewater metagenome)
  ORGANISM  Aminobacteriaceae bacterium
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae.
REFERENCE   1  (bases 1 to 1040)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1040)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:19:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,224
            CDSs (total)                      :: 3,198
            Genes (coding)                    :: 3,184
            CDSs (with protein)               :: 3,184
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1040
                     /organism="Aminobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_174577_length_1040_cov_2.195939"
                     /isolate="AMR_MDS_3026"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:3034609"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1040)
                     /locus_tag="P5201_15900"
     CDS             complement(<1..>1040)
                     /locus_tag="P5201_15900"
                     /inference="COORDINATES: protein motif:HMM:NF012430.2"
                     /note="negatively supercoils closed circular
                     double-stranded DNA; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit B"
                     /protein_id="HRW00085.1"
                     /translation="VSGGLHGVGASVVNALSEKLELFVRRGGREYSMTFMRGDVFKEL
                     SSKPAKTPGTGTTVRFYPDPLIFKTGIVFDPKLVERRLREISYLVPGLSLTLETVNAE
                     GETVRETFMNKDGIEAMTNRLAKNTTPLFEGTVAFTGTSGDTEVAFALKYLDDYSERM
                     YSFVNLIPTAEGGTHVAGFRAALTRAVNEAAKTEKLLKAGEGNILGDDLREGLLCVLS
                     VKLPNPQFEGQTKGKLGNGETTGAVSTLVYDKLSYFFANEGGGILRIIVDKALSTRRA
                     REAAKRAKELVRSKGGGGRDAGLPGKLADCISRKPAEAELFIVEGDSAGGSAKQGRDR
                     KFQAILPLRGKI"
BASE COUNT          163 a          370 c          306 g          201 t
ORIGIN      
        1 gaatcttgcc gcgtaacggg aggatagcct gaaacttcct gtcccgcccc tgcttggcgc
       61 tgcctccggc gctgtcgccc tcgacgatga agagctccgc ctcggcgggc ttccgcgaga
      121 tgcagtcggc gagctttccg ggaagccctg cgtcccttcc gccgcccccc ttgctcctga
      181 ccagctcctt cgcccgcttg gcggcctccc tcgccctcct cgtggagagc gccttgtcga
      241 cgatgatccg gaggatcccc ccgccctcgt tcgcgaagaa gtaggagagc ttgtcgtaca
      301 ccagcgtgct caccgcgccg gtcgtctcgc cgttgccaag ctttcctttc gtctgcccct
      361 cgaactgcgg gttcggaagt ttcaccgaga ggacgcagag cagcccttcg cgaaggtcgt
      421 cgccgaggat gttgccctcc cccgccttca ggagcttctc cgtcttggcg gcttcgttca
      481 ccgccctcgt cagggcggcc cggaagcctg cgacgtgcgt ccccccctcg gcggtcggaa
      541 tcaggttgac aaaagaatac atcctctcac tgtagtcgtc gaggtacttc aaagcgaacg
      601 cgacctcggt gtcgccggag gttcccgtaa acgcgaccgt cccctcgaag agcggcgtgg
      661 tgttcttcgc gaggcggttc gtcatcgcct cgatgccgtc cttgttcatg aaagtttcgc
      721 ggaccgtctc cccctccgcg ttcaccgtct ccagcgtcag cgagagcccc ggcacgaggt
      781 aggatatctc gcgcaggcgg cgttcgacga gcttcggatc gaagacgatc cccgtcttga
      841 agatcaacgg atcggggtaa aaccgcaccg tcgtgccggt accgggggtc ttcgccggct
      901 tcgacgaaag ctccttgaaa acgtcgccgc gcatgaaggt catggagtac tcccggccgc
      961 cccgccgcac gaagagttcc agcttctcgg agagggcgtt caccaccgac gcgcccaccc
     1021 cgtgaaggcc gcccgaaacg
//
LOCUS       DAOTIL010001090         1038 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminobacteriaceae bacterium isolate AMR_MDS_3026
            NODE_174968_length_1038_cov_5.128179, whole genome shotgun
            sequence.
ACCESSION   DAOTIL010001090 DAOTIL010000000
VERSION     DAOTIL010001090.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868050
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminobacteriaceae bacterium (wastewater metagenome)
  ORGANISM  Aminobacteriaceae bacterium
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae.
REFERENCE   1  (bases 1 to 1038)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1038)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:19:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,224
            CDSs (total)                      :: 3,198
            Genes (coding)                    :: 3,184
            CDSs (with protein)               :: 3,184
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1038
                     /organism="Aminobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_174968_length_1038_cov_5.128179"
                     /isolate="AMR_MDS_3026"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:3034609"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            331..>1038
                     /locus_tag="P5201_15905"
     CDS             331..>1038
                     /locus_tag="P5201_15905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005660727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HRW00086.1"
                     /translation="MKAILAGTLETGRGTTQKNATIFLDKGKIVDIAEGLDPKGASEV
                     LDASAFVVTPGLIDAHTHIGTYCEGFPVGMADANDMVHPIAPQLRILDAIYQDDTAFE
                     DALAGGVTCVQTLPGSGNVIGGQGAIIKTSTSVHGRKKVVEEMLVRAPSSMKAALGEN
                     PIRVYTEKGKLPNTRMGNAALMRQALVDAENYRNKQNQAKAKNEAGERNLQHEAMLPV
                     LDGELPLCVHAEGEFAVE"
BASE COUNT          223 a          339 c          292 g          184 t
ORIGIN      
        1 caaagatctc cgcctccacg ccaccctcgc ccccctgctt cgggcagtcc gcccgcgtca
       61 gcaaagaaga gcgcaccacg cctatgtgga acagctgcat ttgctcctcg ttcatcctct
      121 ctccctcttt tcctttatcc ccgggagaat gcacacaccc cgcccggtct tcattgtatt
      181 gtatcgcgca ctcgaaattg gtcactattc gtcattatct tcgtacgctt cttatgcttg
      241 ctttgccttt tcccagagcc gtgtatgctg tagaataaag aaagatcaat agtatccgat
      301 gctccggcat cgcaaggagg tactcttttc atgaaggcaa ttctggccgg tacgcttgaa
      361 accggacgcg gcacaacaca aaagaacgcg accatcttcc tggacaaggg aaagatcgtc
      421 gacatcgcgg aagggctcga ccctaaaggc gcctcggagg tgctcgacgc ctccgcgttc
      481 gtcgtcaccc ccgggctcat cgacgcgcac acgcacatcg ggacctactg cgaagggttc
      541 cccgtcggca tggccgacgc caacgacatg gtgcacccga tcgctccgca gcttcgcatt
      601 ctcgacgcga tctatcagga cgacacggcg ttcgaggacg ccctcgcggg cggcgtcacc
      661 tgcgtccaga cgctccccgg cagcggcaac gtcatcggcg gacagggagc gatcatcaag
      721 acgagcacct cggtacacgg acggaagaag gtcgtcgagg agatgctggt gagggcgccc
      781 tcttccatga aggcggcgct cggggaaaat ccgatccgcg tctacacgga aaaggggaag
      841 cttcccaaca cccgcatggg aaacgccgcg ctgatgcggc aggccctcgt cgacgcggag
      901 aactaccgga acaagcagaa ccaggcgaag gcgaagaacg aggccggaga gcgcaacctt
      961 cagcacgagg cgatgctccc ggtgctcgac ggcgaactcc ccctctgcgt gcacgcagag
     1021 ggggagttcg ccgtcgag
//
LOCUS       DAOTIL010001091         1037 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminobacteriaceae bacterium isolate AMR_MDS_3026
            NODE_175330_length_1037_cov_2.492872, whole genome shotgun
            sequence.
ACCESSION   DAOTIL010001091 DAOTIL010000000
VERSION     DAOTIL010001091.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868050
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminobacteriaceae bacterium (wastewater metagenome)
  ORGANISM  Aminobacteriaceae bacterium
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae.
REFERENCE   1  (bases 1 to 1037)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1037)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:19:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,224
            CDSs (total)                      :: 3,198
            Genes (coding)                    :: 3,184
            CDSs (with protein)               :: 3,184
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1037
                     /organism="Aminobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_175330_length_1037_cov_2.492872"
                     /isolate="AMR_MDS_3026"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:3034609"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            378..>1037
                     /locus_tag="P5201_15910"
     CDS             378..>1037
                     /locus_tag="P5201_15910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014163973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malic enzyme-like NAD(P)-binding protein"
                     /protein_id="HRW00087.1"
                     /translation="MRSEEVYAKSLELHRAKKGKVAIASKMPVNSMADLALAYTPGVA
                     EPCREIGRNKDAIYDVTSKGNMVAVITDGTAVLGLGDIGPDAALPVMEGKSILFKRFA
                     DIDAFPICINSKEVDEIVRTSALIASSFGGVNLEDIAAPRCFEVEKKLQELCDIPIFH
                     DDQHGTAVIVLSGILNALKVVGKKLETVKIVMTGAGAAGTAICKFLMSAGAKNVILCD
                     RS"
BASE COUNT          257 a          282 c          293 g          205 t
ORIGIN      
        1 ggccgggtgg aaaagaggtc gggacgacgc atttcaaccg tcgccgtcat gaaagacgat
       61 gacgggaaga aagtagccga ggcaaacggc cttttcctga agattcggca gaaggaagcg
      121 ccggaaaaca aaggagagga agaaatacca cccatgtgag ttgcatgcat ggtcgtcatg
      181 catgcaacgg gaggaacaca aacgcccatc gctgcacttt ttcttatctc cgtccgttct
      241 ctcttcagat atattttcga ttttgcacaa attgctgaag aaaatggtgc agttttcgct
      301 gtggatggta aaatagtttc gttgttctgc gctccggagt acactcaata agcggaaatt
      361 ttaaaggagg ctgtttcatg agaagcgagg aagtctacgc aaaatcactc gaactgcacc
      421 gggctaaaaa aggcaaggtc gctatcgcaa gcaagatgcc ggtcaactcc atggccgact
      481 tggccctggc ctataccccg ggagtcgccg agccctgccg cgagatcggc cggaacaagg
      541 atgcgatcta cgacgtgaca tccaagggga acatggtcgc cgtcatcacc gacggcaccg
      601 cagtactggg cctgggcgat atcggccccg acgcggccct gcccgtcatg gaagggaaat
      661 ccattctttt caagcgcttt gccgatatcg acgcgttccc catctgcatc aactcgaaag
      721 aggtggacga gatagtgcgc acatccgccc tcatcgcctc ttccttcggc ggcgtcaacc
      781 ttgaggacat cgcggcgccg cgctgcttcg aggtggagaa aaagctccag gagctctgcg
      841 acatccccat cttccacgac gaccagcacg gtaccgcagt catcgttctc tccggaattc
      901 tcaacgcgct gaaggtggtc gggaagaaac tcgaaacagt caagatcgtc atgaccggag
      961 cgggcgcagc gggaacggcg atctgcaaat tcctgatgtc cgccggggcg aagaatgtga
     1021 ttttgtgcga ccgcagc
//
LOCUS       DAOTIL010001092         1035 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminobacteriaceae bacterium isolate AMR_MDS_3026
            NODE_175760_length_1035_cov_5.163265, whole genome shotgun
            sequence.
ACCESSION   DAOTIL010001092 DAOTIL010000000
VERSION     DAOTIL010001092.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868050
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminobacteriaceae bacterium (wastewater metagenome)
  ORGANISM  Aminobacteriaceae bacterium
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae.
REFERENCE   1  (bases 1 to 1035)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1035)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:19:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,224
            CDSs (total)                      :: 3,198
            Genes (coding)                    :: 3,184
            CDSs (with protein)               :: 3,184
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1035
                     /organism="Aminobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_175760_length_1035_cov_5.163265"
                     /isolate="AMR_MDS_3026"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:3034609"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(145..>1035)
                     /locus_tag="P5201_15915"
     CDS             complement(145..>1035)
                     /locus_tag="P5201_15915"
                     /inference="COORDINATES: protein motif:HMM:NF020939.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043138 - 3'-5' DNA helicase activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RQC domain-containing protein"
                     /protein_id="HRW00088.1"
                     /translation="GRAGRDGSPAECVLFYGGQDVFTNQFFIEKERENEELDEETLRK
                     VRESERNRLRKMVAYCHTTDCLRAAILGYFGDDAPSACGHCSNCLRDFEQEDITIPAQ
                     KILSCVLRMKERYGVSLLVSTLRGSKSERVLKLGLGELSTYGIMKDESERSLRVKIDY
                     LLSRGYISLTDDEFPVVKTTPLAKGVLFRGEKLAMPVAKQEPKPLAAEPKGVSMLMEP
                     TDLELFDKLRALRKEIAGAQGVPAFVVFTDATLRDMCEKLPQTPDELLQVSGVGYAKR
                     EKYGAAFLELLREHSTASVP"
BASE COUNT          202 a          326 c          286 g          221 t
ORIGIN      
        1 cttcgttgaa cctgaacagc cctcgcatac gcacccctcc attcgaagaa tatgttcgtc
       61 tattatactc tttttactac aacaaaaaga gcgcatcccc aaaaaaggac acgctcttca
      121 cacacaaaat ctgtgattct gtttttacgg aacggaagca gtcgagtgtt cgcgaagcag
      181 ttccaaaaag gccgcgccgt acttttcccg tttggcgtac cccacgccgc tcacctggag
      241 cagttcgtcc ggcgtctgcg gcagcttctc gcacatgtcg cgaagcgtcg cgtcggtaaa
      301 gaccacgaag gcgggaacgc cctgcgcgcc ggcgatctct ttccggaggg cgcgcagctt
      361 gtcgaagagt tcgaggtcag tcggctccat gagcatgctc acacctttcg gctcggcggc
      421 aagcggtttc ggctcctgct tcgccacggg catcgcgagc ttctctcccc gaaagagcac
      481 tccctttgcc aacggagtgg tcttcaccac gggaaattcg tcgtccgtga gcgaaatata
      541 ccctcgcgaa agcagatagt ctatctttac gcgcaacgaa cgctcgctct catccttcat
      601 tatgccgtag gttgaaagtt cgccaaggcc cagcttgagc acgcgctcgc tcttgctgcc
      661 gcgcagcgtg ctcacgagca gcgagacccc gtagcgctcc ttcatcctga gcacgcagga
      721 gaggatcttc tgcgcgggga tggtgatatc ctcctgttcg aagtcgcgga ggcagttcga
      781 gcagtgaccg caggcggagg gggcatcgtc gccgaagtac cccaggatag cggcgcggag
      841 gcaatccgtc gtgtggcagt aggcgaccat cttgcgcagc ctgttccgct cggactcgcg
      901 caccttccgc agcgtctcct cgtcgagttc ttcgttctcg cgctcttttt ctatgaagaa
      961 ctggtttgtg aacacgtcct gaccgccgta gaagagcacg cactccgcgg gggagccgtc
     1021 acgtccggcg cggcc
//
LOCUS       DAOTIL010001093         1033 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminobacteriaceae bacterium isolate AMR_MDS_3026
            NODE_176336_length_1033_cov_3.274029, whole genome shotgun
            sequence.
ACCESSION   DAOTIL010001093 DAOTIL010000000
VERSION     DAOTIL010001093.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868050
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminobacteriaceae bacterium (wastewater metagenome)
  ORGANISM  Aminobacteriaceae bacterium
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae.
REFERENCE   1  (bases 1 to 1033)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1033)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:19:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,224
            CDSs (total)                      :: 3,198
            Genes (coding)                    :: 3,184
            CDSs (with protein)               :: 3,184
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1033
                     /organism="Aminobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_176336_length_1033_cov_3.274029"
                     /isolate="AMR_MDS_3026"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:3034609"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1033)
                     /locus_tag="P5201_15920"
     CDS             complement(<1..>1033)
                     /locus_tag="P5201_15920"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRW00089.1"
                     /translation="ASTLPTPYEELLFPENPEMRELREETSLPPLLPVFRRQRENEPV
                     AETRKRVALALGEKLLELHGQPVWNKKNKQMLPAEWRDMTILVPSRSSFAALEETLHP
                     LFGIPVAFEKGKRYFDRGEIGDLTTAIRMLAFPDDRAAALGFLASPFSGLNMEQFARF
                     LGPEAPSLAEVFPETAARIEELRSVARYSGLFAALVLLLKDQSFLGAYPLWNRKSALA
                     NLWKGLDLVREYENVFGNDPAGCAAYVARMAGRKGAGEESTPLGEEEDVVRVLTVHSA
                     KGLEFPITVVMDLNNTPGGGGGRGALVPSALLGAGASRHPEAWSEKSASETGKIARFL
                     EDVEVREEWE"
BASE COUNT          167 a          353 c          340 g          173 t
ORIGIN      
        1 gttcccactc ctccctgact tcgacgtcct ccaaaaaacg ggcgatcttt cctgtctcgg
       61 aggcgctctt ctcgctccat gcctcggggt ggcgcgaagc gcccgcgccc agcagcgcgg
      121 agggaacgag cgcgccccgt ccgccgccgc ctcccggcgt gttgttcagg tccatcacca
      181 ccgtgatcgg gaattccagc cccttcgcgc tgtggacggt gagcacgcgg accacgtctt
      241 cctcctcgcc gaggggcgtg ctctcctcgc cggccccctt gcgccccgcc atgcgggcaa
      301 cgtaggcggc gcacccggcc gggtcgttgc cgaagacgtt ctcgtactcg cggacgaggt
      361 cgagcccctt ccagaggttg gcaagcgcgc tcttccggtt ccacagcggg tacgctccca
      421 ggaacgactg gtctttcagc agcagcacca gcgccgcgaa gaggccggag tagcgcgcga
      481 cgctccgcag ctcctcgatc ctggccgccg tctccggaaa gacctccgca agcgaggggg
      541 cctccggccc gaggaagcgg gcgaactgct ccatgttcag cccggagaag ggggaggcca
      601 gaaaaccgag cgcggccgcc ctgtcgtcgg ggaacgcgag catccggatg gcagtcgtca
      661 gatcgccgat ctctcccctg tcgaagtagc gctttccctt ctcgaaggcg acggggatgc
      721 cgaaaagagg gtgcagcgtc tcctcgagcg cggcgaagga ggagcgcgac ggcacgagaa
      781 tggtcatgtc gcgccactca gcagggagca tctgtttgtt ctttttgttc cagacgggct
      841 ggccgtgcag ctccaacagc ttctctccga gagcaagcgc cacccgtttt cgagtctccg
      901 cgaccggttc gttttcccgc tgacggcgaa agaccggaag gagcggaggg agggacgtct
      961 cctcgcgcag ctcccgcatc tcgggattct ccgggaagag cagctcctcg tagggggtgg
     1021 gaagcgtgga ggc
//
LOCUS       DAOTIL010001094         1033 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminobacteriaceae bacterium isolate AMR_MDS_3026
            NODE_176393_length_1033_cov_2.511247, whole genome shotgun
            sequence.
ACCESSION   DAOTIL010001094 DAOTIL010000000
VERSION     DAOTIL010001094.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868050
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminobacteriaceae bacterium (wastewater metagenome)
  ORGANISM  Aminobacteriaceae bacterium
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae.
REFERENCE   1  (bases 1 to 1033)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1033)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:19:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,224
            CDSs (total)                      :: 3,198
            Genes (coding)                    :: 3,184
            CDSs (with protein)               :: 3,184
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1033
                     /organism="Aminobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_176393_length_1033_cov_2.511247"
                     /isolate="AMR_MDS_3026"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:3034609"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..328
                     /locus_tag="P5201_15925"
     CDS             <1..328
                     /locus_tag="P5201_15925"
                     /inference="COORDINATES: protein motif:HMM:NF013767.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009927 - histidine phosphotransfer kinase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="Hpt domain-containing protein"
                     /protein_id="HRW00090.1"
                     /translation="RVWDRSLLLERVLGDEALAEDVVAGFVSDMPERIAALEKAVAAG
                     DFGETENLSHALKGAAGSVSAEALMRVAKEMEKKSKEGDLDALRTILPAVMWEYERLR
                     SALEKK"
     gene            418..900
                     /locus_tag="P5201_15930"
     CDS             418..900
                     /locus_tag="P5201_15930"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRW00091.1"
                     /translation="MTGRIVTVLVRGVLLLLLCAVSSEAQARMEKEYCNARFGFCFSY
                     PAHFEMDPPPENDDGRILRDGDGCVLTAWGGYNVLEETLAEAMTSRSADFDTVTYSAK
                     GKNWFVLSGYRGSDIVYLKLWVGSGAVNGLEMAYPSALKKEYDAVVTNIVRSFRPGDL
                     "
BASE COUNT          208 a          266 c          357 g          202 t
ORIGIN      
        1 ccgtgtttgg gacaggagcc tcctgctgga gcgggtgctc ggggatgaag ccttggccga
       61 agatgttgtc gcggggtttg tgagcgatat gccggagagg atcgccgctc ttgagaaggc
      121 tgtggcggcc ggcgacttcg gcgaaacgga gaacctgagc cacgctctga aaggggccgc
      181 cggaagcgtc tccgcggaag cgctgatgcg tgtcgcgaag gagatggaga agaaatcgaa
      241 agagggtgac ctcgacgctc tcaggactat tctgccggct gtgatgtggg aatatgaacg
      301 gttgaggtcg gcgctcgaaa agaagtagga agggaaatct ccgagtcttc ttcgtgcgag
      361 cggttccggg cggggacgcg acgaaactgt tctccccgat gcgaagggag gaaaatcatg
      421 actggacgca tagttacagt gttggtgcga ggcgttctgc tccttcttct ctgcgccgtc
      481 tcttcggagg cgcaggcgcg aatggagaaa gagtactgca acgcgcgctt cggcttctgt
      541 ttttcgtacc cggcgcattt cgagatggac ccgcctccgg agaacgacga cggacggatt
      601 ctgcgcgacg gcgatgggtg cgtcctgacg gcgtggggag ggtacaacgt cctcgaagag
      661 acgcttgcgg aggcgatgac ctcgcggagc gccgatttcg acaccgtgac ctacagtgcc
      721 aaggggaaga actggttcgt cctctcgggg tacagaggga gcgacatcgt ctatctcaaa
      781 ctctgggtgg ggtccggcgc ggtcaacgga ctggagatgg cgtacccgtc ggcgttgaaa
      841 aaggagtacg acgcggtggt gacgaacatc gtccgttcgt tccgtcccgg agatttgtag
      901 ccggtccgcg gaaaccgcaa aaaccggaga gaagcgcggg aacgccgcct gtgttttatg
      961 cctccccttt tccgccctct acaaaaactc cagcgggcag aagtccttcg agaaggtctt
     1021 cgttccgtac tgc
//
LOCUS       DAOTIL010001095         1033 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminobacteriaceae bacterium isolate AMR_MDS_3026
            NODE_176421_length_1033_cov_2.335378, whole genome shotgun
            sequence.
ACCESSION   DAOTIL010001095 DAOTIL010000000
VERSION     DAOTIL010001095.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868050
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminobacteriaceae bacterium (wastewater metagenome)
  ORGANISM  Aminobacteriaceae bacterium
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae.
REFERENCE   1  (bases 1 to 1033)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1033)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:19:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,224
            CDSs (total)                      :: 3,198
            Genes (coding)                    :: 3,184
            CDSs (with protein)               :: 3,184
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1033
                     /organism="Aminobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_176421_length_1033_cov_2.335378"
                     /isolate="AMR_MDS_3026"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:3034609"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(111..500)
                     /locus_tag="P5201_15935"
     CDS             complement(111..500)
                     /locus_tag="P5201_15935"
                     /inference="COORDINATES: protein motif:HMM:TIGR02532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein"
                     /protein_id="HRW00092.1"
                     /translation="MKRRNAFTLLEVMIAVAILGLSVAGSLRLIALSVRALEDVRYER
                     RNLALARALWLTASSGKLGERGREREYSWETERFSFERQSDVPEGFFCRKVVLSQADP
                     TSSRSERTKDSFVFFVPDMKIEKERTE"
     gene            complement(515..>1033)
                     /gene="gspC"
                     /locus_tag="P5201_15940"
     CDS             complement(515..>1033)
                     /gene="gspC"
                     /locus_tag="P5201_15940"
                     /inference="COORDINATES: protein motif:HMM:NF041616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein GspC"
                     /protein_id="HRW00093.1"
                     /translation="GKQQKVVLKGQSLKGYELTVVEEDRIVLQKGQASLTVLLRYSTQ
                     PVSGKAAAAAKPARRSVAAARPGQQGSISREMVNQLLMDPLEEMKKFRLRPKFDGESA
                     QGVEVQWLDDKSILTSLGVQKGDVVQSVNGVQIRNMGDIVNVINSLMSGNTFDVQVVR
                     GGQPEMLNYNIK"
BASE COUNT          257 a          298 c          226 g          252 t
ORIGIN      
        1 gaattcgtcg tccgacgctt tttccccctc ctgagcctgc gcaaaaagcg acgacccggg
       61 gatgagaagt acacaaagac ttaaaataag caacattcgt acggtgcggt tcattctgtt
      121 ctctcctttt caatcttcat gtccgggacg aaaaaaacga aagaatcctt cgttctctcc
      181 gaacgggagg acgtcgggtc cgcttgggag agcacgacct tccgacagaa aaaaccttcg
      241 ggaacgtccg actggcgctc gaaagaaaaa cgctccgtct cccatgaata ctctcgttct
      301 ctccctcgct caccgagttt gccggatgaa gcggtcaacc aaagggcacg tgcgagagcg
      361 agattgcggc gctcatagcg aacgtcttca agcgcgcgaa cagatagagc aatcagacga
      421 aggctccctg cgacagaaag cccaagtatt gcaacggcaa tcattacttc gagaagtgtg
      481 aacgcattcc gtcttttcat gtcctgtccg ttcctcactt tatgttgtag ttcagcatct
      541 ccggctgacc gcctctgaca acctgaacat cgaacgtatt accgctcata agggagttaa
      601 taacattaac aatgtcgccc atgttccgta tctgcacccc attcacagat tgaacgacat
      661 ctcctttctg gacgccgaga gatgtcaaaa tacttttgtc atccagccat tgaacttcga
      721 ctccctgagc ggattctccg tcaaactttg ggcgcaggcg gaactttttc atctcctcaa
      781 gaggatccat caagagttga ttcaccatct cccttgatat agagccctgt tgtccaggcc
      841 tcgccgctgc aacagaccgc ctggcaggtt ttgcagcggc ggcagctttc ccactcacag
      901 gttgcgtact gtaacggagc aacaccgtca acgaggcttg ccccttctgc aaaacaattc
      961 gatcttcttc tacaacggta agctcatatc ctttaagaga ctgccctttt aaaacaactt
     1021 tctgctgttt tcc
//
LOCUS       DAOTIL010001096         1032 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminobacteriaceae bacterium isolate AMR_MDS_3026
            NODE_176807_length_1032_cov_1.446264, whole genome shotgun
            sequence.
ACCESSION   DAOTIL010001096 DAOTIL010000000
VERSION     DAOTIL010001096.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868050
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminobacteriaceae bacterium (wastewater metagenome)
  ORGANISM  Aminobacteriaceae bacterium
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae.
REFERENCE   1  (bases 1 to 1032)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1032)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:19:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,224
            CDSs (total)                      :: 3,198
            Genes (coding)                    :: 3,184
            CDSs (with protein)               :: 3,184
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1032
                     /organism="Aminobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_176807_length_1032_cov_1.446264"
                     /isolate="AMR_MDS_3026"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:3034609"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..331
                     /locus_tag="P5201_15945"
     CDS             <1..331
                     /locus_tag="P5201_15945"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRW00094.1"
                     /translation="ADGDAYTRYGRISAATGLPTPLGWFGHQWLWRGGAEEPKERRGD
                     VRALYESADPHATAELVGKYAVRYIVLGSLERERFPSLKEEKLEAAGRVILRTADGSK
                     IVETARP"
     gene            389..>1032
                     /locus_tag="P5201_15950"
     CDS             389..>1032
                     /locus_tag="P5201_15950"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006301732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HRW00095.1"
                     /translation="MEGRSVDYSVIIPVYNEQDVLEATWKRVTDVMRAVGGAYELLFV
                     DDGSRDDSAGILRRICDRDPCSSMISFSRNFGQQAAMSAGIDYSKGRAVVFIDADLQD
                     PPEVIPRMIEKWNEGYAIVYGKRSRREGESVFKKVTAAVFYRLLRAVSEREVLVDVGD
                     FRLIDGAVRDLLRSLPEKNRYIRGLVSWAGFRQTTVEYVREERFAGTTKYSPIKL"
BASE COUNT          210 a          317 c          338 g          167 t
ORIGIN      
        1 agcggacgga gacgcctata cgcggtacgg cagaatatcg gccgcgacgg gcttgccgac
       61 ccctctcgga tggttcggcc atcagtggct ctggagaggc ggcgcggagg agccgaaaga
      121 gcggcgcggg gatgtgcgcg ccctctacga atctgcggac cctcacgcga ccgccgagct
      181 ggtcgggaag tacgcggtgc ggtatattgt actcggttcg cttgaaagag aacgattccc
      241 gtcgctcaag gaagagaagc tggaggctgc ggggcgcgtc atcctccgca ccgcggacgg
      301 ctcgaaaatc gtcgaaaccg cgcgcccgtg agcggcggtc ccgagcgtgc gcaacgacgg
      361 catatcttga agaaaagagg gagagctctt ggaaggacgc agtgtcgact actcggttat
      421 catccccgtc tacaacgagc aggatgtcct ggaggcaacc tggaagcgcg taaccgacgt
      481 catgcgcgcc gtcggcggcg cgtacgaact tctcttcgtg gacgacggca gccgcgacga
      541 ctccgccggc atactgcggc gcatctgcga ccgcgacccc tgttcctcga tgatctcctt
      601 ctcgcggaac ttcggccagc aggcggccat gagcgcggga atcgactact ctaaggggcg
      661 cgccgtcgtc ttcatcgacg ccgaccttca ggacccgccc gaagtgattc cgcggatgat
      721 cgaaaaatgg aacgaagggt acgccatcgt ctacggaaag agaagcagac gcgaagggga
      781 gagcgtcttc aaaaaggtta cggccgccgt cttttacaga ctgcttcggg ccgtctcgga
      841 aagggaagtc ctcgtcgacg tcggcgactt ccgcctgatc gacggcgcag tgcgcgacct
      901 cctgcgctcg ctgcccgaga aaaaccgcta catccgcggc ctggtgagct gggccggatt
      961 ccggcagacc accgtcgagt acgtccggga ggagcggttc gccggaacga ccaagtactc
     1021 cccgatcaaa ct
//
LOCUS       DAOTIL010001097         1028 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminobacteriaceae bacterium isolate AMR_MDS_3026
            NODE_177747_length_1028_cov_2.684481, whole genome shotgun
            sequence.
ACCESSION   DAOTIL010001097 DAOTIL010000000
VERSION     DAOTIL010001097.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868050
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminobacteriaceae bacterium (wastewater metagenome)
  ORGANISM  Aminobacteriaceae bacterium
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae.
REFERENCE   1  (bases 1 to 1028)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1028)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:19:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,224
            CDSs (total)                      :: 3,198
            Genes (coding)                    :: 3,184
            CDSs (with protein)               :: 3,184
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1028
                     /organism="Aminobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_177747_length_1028_cov_2.684481"
                     /isolate="AMR_MDS_3026"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:3034609"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(84..383)
                     /locus_tag="P5201_15955"
     CDS             complement(84..383)
                     /locus_tag="P5201_15955"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRW00096.1"
                     /translation="MKMVWLHCSETLAQETTEILDAAGIEFYSVWRNVLGKDNRGNRT
                     RWDDAVFPGKNWALQFLCTDEAIEELKRRFDELSGDPYVKESGIAMYVAEAERAL"
     gene            complement(380..>1028)
                     /locus_tag="P5201_15960"
     CDS             complement(380..>1028)
                     /locus_tag="P5201_15960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013047715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HRW00097.1"
                     /translation="HGRSLGEITADARKLVETLDIPQGVAIEFGGQIREQSEAFRQMA
                     LLVLLGVLLVYMVMAGQYEAYLDPFVIMFSIPFALTGVVFAFLLTGLYISLQAMLGVV
                     MLVGIVVNNAIVLVDYVNLLRARGTPLRRSLIEAGGRRLRPVLMTTLTTFFGMLPMAV
                     SNAIGSEVWKPLAVSVMGGLVVSTLITLLLVPVMYSFFEEKIRRKGRFAEAVNGR"
BASE COUNT          209 a          332 c          274 g          213 t
ORIGIN      
        1 cccctggcga tgtgctgtat catgagcgtc cctcctttcg gaacttcctt tcattctaca
       61 ccaaccgtcg cccctccccc catttaaagc gctctctccg cttcggcgac atacatggcg
      121 atcccggact ctttgacgta cggatcgccg ctcagttcat cgaatcgccg cttcaactcc
      181 tcgatggcct cgtcggtgca gagaaactgc agcgcccagt tctttcccgg aaagacggcg
      241 tcgtcccaac gtgtcctgtt tccacggttg tccttcccca gaacgttcct ccatacgctg
      301 taaaactcta tgcccgccgc gtcgagtatt tcggtcgtct cctgggcgag cgtttcgctg
      361 cagtgcagcc ataccatctt catcgtccat tcaccgcctc tgcaaagcgt ccttttcttc
      421 gtattttctc ctcgaagaag ctgtacatga ccggaacaag cagcagtgtg atcagggttg
      481 agacgacaag cccccccatg acggagacgg cgagcggttt ccagacctcg gagccgatcg
      541 cgttgctcac ggccatcggc aacatgccga agaaggtggt gagcgtggtc atgagaacag
      601 gccgaagacg ccgtcccccc gcttctatca gcgaccttcg gaggggggtt ccccgcgctc
      661 gcagcagatt cacgtagtcc acgaggacga tggcgttgtt caccacgatg cccacgagca
      721 tcacgacgcc cagcatcgcc tggagagaga tgtagagccc cgtaagcaga aaggcgaaga
      781 ccacgccggt tagggcgaac gggatgctga acatgatgac gaacggatcg agataggctt
      841 cgtactgccc tgccataacc atgtagacca gcagaacgcc gaggaggacg agcagagcca
      901 tctgccggaa cgcctccgac tgttcgcgta tctgccctcc gaattcgatg gcaactccct
      961 gcggtatgtc gagcgtctcc acaagcttac gggcatccgc ggtgatctct ccgagagagc
     1021 gtccgtgg
//
LOCUS       DAOTIL010001098         1028 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminobacteriaceae bacterium isolate AMR_MDS_3026
            NODE_177862_length_1028_cov_1.791367, whole genome shotgun
            sequence.
ACCESSION   DAOTIL010001098 DAOTIL010000000
VERSION     DAOTIL010001098.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868050
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminobacteriaceae bacterium (wastewater metagenome)
  ORGANISM  Aminobacteriaceae bacterium
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae.
REFERENCE   1  (bases 1 to 1028)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1028)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:19:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,224
            CDSs (total)                      :: 3,198
            Genes (coding)                    :: 3,184
            CDSs (with protein)               :: 3,184
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1028
                     /organism="Aminobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_177862_length_1028_cov_1.791367"
                     /isolate="AMR_MDS_3026"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:3034609"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(235..777)
                     /locus_tag="P5201_15965"
     CDS             complement(235..777)
                     /locus_tag="P5201_15965"
                     /inference="COORDINATES: protein motif:HMM:NF022072.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC family protein"
                     /protein_id="HRW00098.1"
                     /translation="MPRVYYSTILSATVEEAWEVVRDFNGLPKWHPGITASELEDACS
                     PDCEACIGSVRHFQLANGEWMREQLLSLSDLDFSVSYTILETGMKLKNYVATLKLTPV
                     TATDETFAEWEAVFDTTDPASEEETAEIVHGVFAEGLENLDDILLEAYEEESGDGCGG
                     CGSGGCSCGSCGCGGDEEEE"
     gene            complement(791..>1028)
                     /locus_tag="P5201_15970"
     CDS             complement(791..>1028)
                     /locus_tag="P5201_15970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005659902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="phosphoenolpyruvate hydrolase family protein"
                     /protein_id="HRW00099.1"
                     /translation="TLEECAPLIDSWAEAAKKVRPDILVLCHGGPIAMPEDASYILKH
                     CKHVHGFYGASSMERLPTETAIKAQTQDFKKITF"
BASE COUNT          178 a          306 c          310 g          234 t
ORIGIN      
        1 cccatgcgat ttttacgcgt tcagaaggga gtgcattttt tcagcggtct ccgtttgaaa
       61 cgcctgcttt cgagtttttc tgcgtcaaag atccgaacgg actgaacgtg cagtttttcg
      121 agcggaaaaa gtaaccctgc ggccgagaag gcggttggag gaatcggaac atgaaccggg
      181 atcccctgag gtacaaggga tcccggttgt gaacgcatta gaaataagga acaactattc
      241 ctcttcttcg tctccgccgc agccgcaact gccgcagctg cacccgccgc ttccgcaccc
      301 cccgcatccg tccccggatt cctcctcgta ggcctccagg agtatgtcgt cgaggttttc
      361 aaggccttcg gcgaagacgc cgtggacgat ctccgccgtc tcttcctcgc tggcggggtc
      421 ggtggtgtcg aagacggcct cccactcggc gaaggtttcg tcggttgcgg tgaccggcgt
      481 gagcttcagg gtggcgacgt agttcttcag cttcatgccg gtctcaagga tcgtgtagct
      541 gaccgagaag tccaggtcgg aaagggacaa aagctgttcg cgcatccact cgccgttcgc
      601 gagctggaag tggcgcacgc ttcctatgca cgcctcgcag tcggggctgc atgcgtcctc
      661 aagttcgctc gccgtgattc ccggatgcca tttcggcagg ccgttgaagt cgcggacaac
      721 ctcccacgcc tcctctacgg tggcggagag tatggtgctg tagtacactc gcggcatatt
      781 cgtttcctcc tcagaatgtg atcttcttga aatcttgcgt ctgtgccttt attgccgtct
      841 ccgtcggcag ccgctccatg gagctcgcgc cgtagaagcc gtggacgtgc ttgcagtgct
      901 tcagaatgta cgaggcgtcc tcgggcatgg cgatcgggcc gccgtgacag aggacgagta
      961 tgtccgggcg gaccttcttc gcggcctcgg cccagctgtc gatgaggggg gcgcactcct
     1021 cgagcgtc
//
LOCUS       DAOTIL010001099         1027 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminobacteriaceae bacterium isolate AMR_MDS_3026
            NODE_177956_length_1027_cov_5.127572, whole genome shotgun
            sequence.
ACCESSION   DAOTIL010001099 DAOTIL010000000
VERSION     DAOTIL010001099.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868050
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminobacteriaceae bacterium (wastewater metagenome)
  ORGANISM  Aminobacteriaceae bacterium
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae.
REFERENCE   1  (bases 1 to 1027)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1027)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:19:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,224
            CDSs (total)                      :: 3,198
            Genes (coding)                    :: 3,184
            CDSs (with protein)               :: 3,184
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1027
                     /organism="Aminobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_177956_length_1027_cov_5.127572"
                     /isolate="AMR_MDS_3026"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:3034609"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..247
                     /locus_tag="P5201_15975"
     CDS             <1..247
                     /locus_tag="P5201_15975"
                     /inference="COORDINATES: protein motif:HMM:NF024854.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="winged helix-turn-helix transcriptional
                     regulator"
                     /protein_id="HRW00100.1"
                     /translation="RSSEESGSPHGLVDGLVDGLVENQREILRIIKSNPQVSKREMAK
                     TLAISTTAIDKNIEILKRKGLLRRIGPAKGGRWEAFE"
     gene            470..688
                     /locus_tag="P5201_15980"
     CDS             470..688
                     /locus_tag="P5201_15980"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRW00101.1"
                     /translation="MLLSIVEERAQERGEKIGEKRGEKRGEKRGATMKALAVAARMLD
                     RGEPREKILDYVEISPKELDKLIESRRN"
     gene            838..>1027
                     /locus_tag="P5201_15985"
     CDS             838..>1027
                     /locus_tag="P5201_15985"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRW00102.1"
                     /translation="MGNRDNAFQRLLADEHLFVRFIRRYLRQHMPDAIDVETLTPEDV
                     YREEADIMLAYMQKEFRKA"
BASE COUNT          261 a          225 c          351 g          190 t
ORIGIN      
        1 gaggtcgtcg gaagaatcag gttccccgca tgggttggta gatgggttgg tagatgggtt
       61 ggtagaaaat cagcgggaga tattgcgcat catcaagtcc aacccgcagg tttcaaagag
      121 agagatggcg aagacgcttg ctatcagcac gactgccatc gataaaaaca ttgaaatact
      181 gaagagaaag gggcttctca ggcgtatagg tccggccaag ggcggtcgct gggaggcgtt
      241 tgaatagatt tcgtgccgta agcgccatct tgagcctcct tatcttgcag ctctcttgct
      301 gattattttc gcatgactca actaggacac tctggacgcc aaggggtgga cgcacgacga
      361 gaagctggcg atcttccggc tgacggatac gctgctgcac ccgaagagcc cggaactgcg
      421 gaagtcgtac gacgaattca gggaggaacg gaggaaggag ggaaagaccg tgttgttgag
      481 catagtcgag gaaagagcgc aggagagagg cgagaaaata ggcgagaaga gaggcgagaa
      541 aaggggcgag aagaggggcg ccacgatgaa ggcgctcgcc gtagccgcca ggatgctgga
      601 caggggagag ccccgcgaga agattttgga ctatgtcgag atatccccga aggaactcga
      661 taagctgatc gagtcgcgcc ggaactgagc gagaaccctt cgtcgtcgag gggaaacgcg
      721 acttcgcccg ggaacgaggc gtgcgttgcg tcgttgccgg cgggtcgaag ggaattcggc
      781 gactgatgga gcgtatcgac gatttcctgc aaaatactgt aagtgaaggg gagcccgatg
      841 gggaacaggg ataatgcgtt ccagcggctt ttagcggacg aacatctctt cgtgaggttc
      901 attcgccgct atctgagaca gcacatgccg gatgcgatcg acgtggagac tttgacgcct
      961 gaagatgtgt accgggagga ggcggatatc atgctggcct acatgcagaa ggagttcagg
     1021 aaggcgc
//
LOCUS       DAOTIL010001100         1025 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminobacteriaceae bacterium isolate AMR_MDS_3026
            NODE_178697_length_1025_cov_1.597938, whole genome shotgun
            sequence.
ACCESSION   DAOTIL010001100 DAOTIL010000000
VERSION     DAOTIL010001100.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868050
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminobacteriaceae bacterium (wastewater metagenome)
  ORGANISM  Aminobacteriaceae bacterium
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae.
REFERENCE   1  (bases 1 to 1025)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1025)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:19:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,224
            CDSs (total)                      :: 3,198
            Genes (coding)                    :: 3,184
            CDSs (with protein)               :: 3,184
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1025
                     /organism="Aminobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_178697_length_1025_cov_1.597938"
                     /isolate="AMR_MDS_3026"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:3034609"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>1025
                     /locus_tag="P5201_15990"
     CDS             <1..>1025
                     /locus_tag="P5201_15990"
                     /EC_number="2.8.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006584009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="CoA transferase"
                     /protein_id="HRW00103.1"
                     /translation="DPGRTLGRHAGGYSSYFESLNRNKRSLCLDLSKPGAVEVVHRLV
                     ASVDIVVENFRPGTMEKLGLGYEDLRKVNPRLIYAASSGFGQTGPYSRRPAMDVIIQG
                     MGGMMSVTGPDEHTPTKVGSSIADIFAGLFTTIGILSAVNSRMKTGRGQLVDVAMLDC
                     MVAVLENAVSRYLVTGEIPRPMGNKHPAISPFATLATADGVMNIAVGNDDIWHRFCGV
                     LGMEEFENDPRFADNPGRVKNFDALKVLMEERTVRQTTEYWLSILEKNHVPCGPINDM
                     AHVVNDPQVLARKMIVDVLHPIAGATKIPGVPIKFSETPSEIEAPAPVLGEHTEKILT
                     SYLGLSA"
BASE COUNT          213 a          317 c          305 g          190 t
ORIGIN      
        1 gtgacccggg ccgcacgctt gggcgccatg ccggcgggta ctcctcttac ttcgaatcgc
       61 tgaaccggaa caagcgaagc ctctgcctgg acctctcaaa gccgggcgcg gtcgaggtcg
      121 tccacagact tgtagcgagc gtcgacatcg tcgtcgagaa cttccggccc gggacgatgg
      181 aaaagctcgg cctggggtat gaagatctca gaaaggtgaa tcctcgcctg atctatgcgg
      241 caagctccgg gttcggtcag accggcccgt acagcagacg tccagccatg gatgtcatta
      301 tccagggtat gggcggcatg atgagcgtca ccggaccgga cgagcacact cccacgaagg
      361 tgggcagctc gattgccgac atcttcgcgg gccttttcac caccatcggc attctctccg
      421 cggtgaactc gcgcatgaag acaggcagag gccagctcgt cgacgtcgcc atgctcgact
      481 gtatggtggc cgtgctggaa aacgccgtct cccgctacct cgtgaccgga gagattcccc
      541 gtccgatggg caacaagcac cctgcgatct cgcctttcgc cacccttgca accgcggacg
      601 gcgtgatgaa catcgctgtg ggcaacgacg acatctggca cagattctgc ggcgtcctcg
      661 ggatggagga gttcgagaat gatccccgct ttgcggacaa cccggggcgg gtgaagaatt
      721 tcgatgcgct gaaagtgctc atggaggagc gtacagtacg tcagacgacg gaatactggc
      781 tttccatact tgagaaaaat cacgttccct gcggtccgat caacgatatg gcgcacgtcg
      841 tcaacgaccc gcaggtgctt gcgcgaaaga tgatcgtcga cgtactccac ccgatagccg
      901 gcgcaacgaa gatccccggc gttccgatca agttctcgga aacgccctct gaaatcgagg
      961 cccccgcgcc ggtgctcggc gagcataccg agaagatact gacgtcgtat ctcggtctct
     1021 ccgcc
//
LOCUS       DAOTIL010001101         1023 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminobacteriaceae bacterium isolate AMR_MDS_3026
            NODE_179141_length_1023_cov_2.487603, whole genome shotgun
            sequence.
ACCESSION   DAOTIL010001101 DAOTIL010000000
VERSION     DAOTIL010001101.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868050
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminobacteriaceae bacterium (wastewater metagenome)
  ORGANISM  Aminobacteriaceae bacterium
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae.
REFERENCE   1  (bases 1 to 1023)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1023)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:19:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,224
            CDSs (total)                      :: 3,198
            Genes (coding)                    :: 3,184
            CDSs (with protein)               :: 3,184
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1023
                     /organism="Aminobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_179141_length_1023_cov_2.487603"
                     /isolate="AMR_MDS_3026"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:3034609"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..990)
                     /locus_tag="P5201_15995"
     CDS             complement(<1..990)
                     /locus_tag="P5201_15995"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008170 - N-methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006306 - DNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-6 DNA methylase"
                     /protein_id="HRW00104.1"
                     /translation="MKKVQWFFEERGILSPERRINELLRVKTVLNALANEKLAKEDEE
                     TLFLAMKELTEKEFGFFPADRSDFRELFETLKRVDALEFAADVFAEDRKSWLGSPLLT
                     RYVVERIESLKPKSVLIAEAEKHLAGLGEMRRKCEGIELTLTTENAAVYRLLSLEFAE
                     TPDVIVRQESIYKESPDALKYEYIHAFPAVGRRREAGEGTGKTIPANELDEIAVENLL
                     RRLAPGGTMDAVVPAKMTFGGGGYKKLRSLLAESFGVESLFNLPEKLFRPVLALRLCL
                     LSVSERKRDTVLIGTYEEDGDSLAVTLKMEMPSREFLSRGDWRVELLLSEEDEA"
BASE COUNT          199 a          326 c          266 g          232 t
ORIGIN      
        1 agcctcgtcc tcctccgaga gaagaagctc gaccctccag tcgccgcgcg agagaaactc
       61 ccgcgaaggc atctccattt tcagcgttac cgcaagcgaa tccccgtcct cttcgtatgt
      121 gccgatcagg acggtatccc gcttcctctc ggagacggag agaagacaga gccgcagagc
      181 gaggacgggg cgaaagagct tctccggcag attgaagaga gattcgaccc cgaagctctc
      241 cgccaggagg gaacggagct tcttgtatcc tccgccgccg aaggtcatct tcgccggaac
      301 gaccgcatcc atcgttcctc cgggagcgag gcggcgcagg agattctcga cggcgatttc
      361 gtccagttca ttcgccggga tcgtctttcc ggtcccctcg cccgcctctc tcctgcgccc
      421 gacggcggga aaagcgtgga tatattcgta tttcaaagcg tcgggactct ccttgtagat
      481 ggattcttgc cggacgatca catccggcgt ttcggcgaac tccagagaga gcagtctgta
      541 cacggcggcg ttctcggtcg tcaacgtcag ttcgatgccc tcgcacttcc gccgcatctc
      601 ccccagtcct gccagatgtt tttcggcctc ggcgatcagg acgctcttcg gcttcagcga
      661 ctctatcctt tcgacgacgt agcgagtcaa aagcggcgat ccgagccacg atttacggtc
      721 ttcggcgaag acgtccgccg cgaattcgag agcatcgact ctcttcagtg tctcgaagag
      781 ttcccggaaa tccgagcggt ccgcggggaa gaatccgaac tccttctccg taagctcctt
      841 catggcgaga aagagcgtct cttcgtcttc tttcgctaat ttctcgttcg cgagcgcatt
      901 cagtaccgtc ttcacccgca gcagttcgtt aattcgtctt tcaggcgaga ggatccccct
      961 ctcttcaaag aaccattgta cttttttcat agccttcgcc tcctcgatgc aaatatgcta
     1021 cac
//
LOCUS       DAOTIL010001102         1023 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminobacteriaceae bacterium isolate AMR_MDS_3026
            NODE_179143_length_1023_cov_2.455579, whole genome shotgun
            sequence.
ACCESSION   DAOTIL010001102 DAOTIL010000000
VERSION     DAOTIL010001102.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868050
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminobacteriaceae bacterium (wastewater metagenome)
  ORGANISM  Aminobacteriaceae bacterium
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae.
REFERENCE   1  (bases 1 to 1023)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1023)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:19:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,224
            CDSs (total)                      :: 3,198
            Genes (coding)                    :: 3,184
            CDSs (with protein)               :: 3,184
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1023
                     /organism="Aminobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_179143_length_1023_cov_2.455579"
                     /isolate="AMR_MDS_3026"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:3034609"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>1023
                     /locus_tag="P5201_16000"
     CDS             <1..>1023
                     /locus_tag="P5201_16000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009164529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HRW00105.1"
                     /translation="EDTLIVFDEIQECPDALTALKYFCENAPEYHLACAGSLLGVMLA
                     KTASFPVGKVDFLNLNPMTFTEFLMANGNGNLVEYLDGVEDVSPLPDAFFNPLYENLK
                     MYFITGGMPESVKIWTLQRDTDAMQQALSNVLNAYERDFAKHPERKDVPKISMIWNSI
                     PSQFAKENKKFLYRVVKEGARAREYEDALQWLCNSGLVFKTYRNELPKLPLKAYDDIS
                     AFKIYMVDTGLLRRMAALDPSVFAERERLFLEFNGALSENYIVQALTDQFEVPPRYWT
                     KEGVPRYEVDFLLQRKDAVYPVEVKAGGNVESRSQKKYKEIYGADVPLRIRFSLRNLK
                     LDGDVLN"
BASE COUNT          249 a          307 c          273 g          194 t
ORIGIN      
        1 gaagacaccc tcatcgtttt cgacgagatc caggagtgcc ccgacgcgct caccgccttg
       61 aagtactttt gtgaaaacgc ccccgaatac cacctcgcat gtgcaggctc ccttctcggc
      121 gtcatgctcg ccaaaacggc ttcatttccg gtggggaagg tggactttct caacctgaac
      181 cccatgacgt tcaccgagtt cctgatggcg aacggaaacg ggaatctcgt cgaatacctc
      241 gacggcgtcg aggacgtgag ccctctgccg gacgcatttt tcaacccttt gtacgagaac
      301 ctgaaaatgt acttcatcac cggaggaatg cccgagtccg taaaaatctg gacgctgcag
      361 cgcgacaccg acgcgatgca gcaggcgctc tccaacgtgc tcaacgccta cgaacgcgac
      421 ttcgccaaac accccgagcg gaaagacgtt cccaagatct ccatgatatg gaactccatc
      481 ccctctcagt tcgccaaaga gaacaaaaag ttcctgtaca gggtggtcaa ggagggcgcg
      541 cgggcgaggg agtacgaaga cgctctgcaa tggctctgca actccgggct ggtgttcaag
      601 acatacagaa acgaactgcc gaaactcccg ctgaaggcgt acgacgatat ctccgcgttc
      661 aaaatctaca tggtcgacac agggttactg cgccggatgg cggctctcga tccctccgtc
      721 tttgcggaac gcgagcgtct tttcctcgaa ttcaacggcg cgctgagcga aaactatatc
      781 gtgcaggcgc tgaccgacca gttcgaagtg ccgccgagat actggacaaa agagggcgtc
      841 cctcggtacg aagtggattt tctgctgcaa cgaaaggacg ccgtctaccc cgtcgaagtg
      901 aaggcgggcg gcaacgtgga gagccgcagt cagaaaaaat acaaagagat ctacggcgcc
      961 gacgttccac tccgcatccg cttctccttg cgtaatctca agctcgacgg agacgtgctg
     1021 aac
//
LOCUS       DAOTIL010001103         1021 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminobacteriaceae bacterium isolate AMR_MDS_3026
            NODE_179602_length_1021_cov_4.146998, whole genome shotgun
            sequence.
ACCESSION   DAOTIL010001103 DAOTIL010000000
VERSION     DAOTIL010001103.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868050
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminobacteriaceae bacterium (wastewater metagenome)
  ORGANISM  Aminobacteriaceae bacterium
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae.
REFERENCE   1  (bases 1 to 1021)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1021)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:19:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,224
            CDSs (total)                      :: 3,198
            Genes (coding)                    :: 3,184
            CDSs (with protein)               :: 3,184
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1021
                     /organism="Aminobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_179602_length_1021_cov_4.146998"
                     /isolate="AMR_MDS_3026"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:3034609"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..897
                     /locus_tag="P5201_16005"
     CDS             <1..897
                     /locus_tag="P5201_16005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011365616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="integrase core domain-containing protein"
                     /protein_id="HRW00106.1"
                     /translation="AILWELEDMDISPLPSLRTINRILARNELTHRRTGRYSPKGTPY
                     PALPSAGVNDTHQADLVGPCYLKGPIRFYSLNVVDLATARCGLYPSVSKGAKSIMNGL
                     WNIWKRLGIPDRVQVDNTFTFAGSPKYPRSMGPLIRLCLHSGVEPWFIPPSEPWRNGS
                     VERFNDFYQQSFLGKVEMFSVDELKNGSLAFENRHNTTWRYSKIGGKTPVKALGSMKS
                     KLRFPDPEAPPPDWRKEPEKGRYHFVRLIRSDLRLNIFGESFSVPPELEFEYAVATID
                     VKEQKLTISHDGKPVKQIEYKR"
BASE COUNT          243 a          273 c          293 g          212 t
ORIGIN      
        1 gccatcctct gggagctgga ggacatggat atttcgcctc tgccgtcgct ccgcaccatc
       61 aacaggattc tcgcgcggaa cgaactgacg catcgaagaa cggggcgcta ttcccccaag
      121 ggaacgccgt atccggcgct tccttccgcc ggggtcaacg acacccatca ggcggatctc
      181 gtcggaccgt gctacctgaa ggggccgatt cgcttctaca gtctcaacgt ggtcgatctg
      241 gccactgcga ggtgcggact gtacccctcg gtctcgaaag gagcgaagag catcatgaac
      301 ggcctctgga atatctggaa acgtctgggt attccggaca gagtccaggt ggacaatacc
      361 ttcaccttcg ccggcagtcc caaatatccg cgaagcatgg ggcctcttat ccgcctctgc
      421 ctgcacagcg gagtggagcc gtggttcatt ccgccgtcgg agccatggag gaacggcagc
      481 gtggagcgat tcaacgactt ttaccagcag agttttctcg ggaaggtcga gatgttctcc
      541 gtagatgagc tgaagaacgg gtctcttgct ttcgagaaca ggcacaacac cacgtggcgt
      601 tacagcaaga tcggcggaaa gactccggtg aaagcgttgg gctcgatgaa gagtaagctt
      661 cgattccccg atccggaggc gcctccgccg gattggcgga aagaaccgga aaagggcaga
      721 taccatttcg tgcgtctgat ccgaagcgac ttgaggctga acatattcgg agagagcttt
      781 tcggtgccgc ctgaactgga gttcgaatat gccgtcgcca ctatagatgt caaggagcag
      841 aagcttacaa tttctcatga cggaaaaccg gtgaagcaaa tagagtataa gcgttaaagc
      901 gaaaaagaat accagtctac gatgtggtgg cacatttttt tgtctacgat gtgctggcac
      961 ttaacacata gaaattgaaa gtgtcctgtg cgacacggcc cgtatgacgc cctccctgag
     1021 c
//
LOCUS       DAOTIL010001104         1020 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminobacteriaceae bacterium isolate AMR_MDS_3026
            NODE_180046_length_1020_cov_1.834197, whole genome shotgun
            sequence.
ACCESSION   DAOTIL010001104 DAOTIL010000000
VERSION     DAOTIL010001104.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868050
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminobacteriaceae bacterium (wastewater metagenome)
  ORGANISM  Aminobacteriaceae bacterium
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae.
REFERENCE   1  (bases 1 to 1020)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1020)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:19:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,224
            CDSs (total)                      :: 3,198
            Genes (coding)                    :: 3,184
            CDSs (with protein)               :: 3,184
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1020
                     /organism="Aminobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_180046_length_1020_cov_1.834197"
                     /isolate="AMR_MDS_3026"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:3034609"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..662
                     /locus_tag="P5201_16010"
     CDS             <1..662
                     /locus_tag="P5201_16010"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRW00107.1"
                     /translation="MPPAAKRKQDTREDVCYSGKDTALEKEERARSETKLAEKEAVFR
                     KNPADISAVLNLADAYAAMNDAASVSLALALYKGLAESSPSDMILARLADAYARLSLF
                     DRAFTTALRRSWNPLVSPEGPVSQILLLAGAGGDWERGIDCLQQILAGREDEREVTLL
                     ALAALYVEKSRVLSEKEKRLRDEYRSAAAACLDEAEASLPRSSGMWENLLRLRKELEE
                     L"
     gene            659..>1020
                     /locus_tag="P5201_16015"
     CDS             659..>1020
                     /locus_tag="P5201_16015"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRW00108.1"
                     /translation="MSRVRINGVRLACCLSLLLSALFFPLSSSLASEDASLLKAAERV
                     ASGKGSSADEALLFMENRRVNHLAMEGKLDGSVYQKTQELYDGKNRALAAEAAREAGF
                     ATEAGGKKIYKPGTDTDI"
BASE COUNT          221 a          281 c          314 g          204 t
ORIGIN      
        1 agatgccccc tgcagcgaag aggaaacagg atacgcgaga ggatgtctgc tactccggaa
       61 aggataccgc tctcgagaaa gaggaacgtg cccggagtga aacaaaactc gcagaaaaag
      121 aagccgtatt ccgtaagaac cccgccgaca tatccgccgt tcttaatctc gcggacgcct
      181 atgccgcaat gaacgacgcc gcgtcagtct cgcttgcgct tgcactgtac aaaggtctgg
      241 cggaatcctc tccgtccgat atgatacttg cccgccttgc ggatgcttat gcgcgtctct
      301 ccctcttcga ccgagcgttc acgacggcgc ttcgacgttc gtggaatccg ctcgtttctc
      361 cggagggacc tgtctcacag attcttcttc tcgccggggc cggcggggac tgggaacgtg
      421 gtatcgattg ccttcaacaa attctggccg ggagggagga tgaacgcgag gtcactcttc
      481 tggccttggc ggctctctat gtagagaagt ccagagtttt aagcgaaaaa gaaaaaaggc
      541 tgcgcgacga gtatcgaagt gcggcggctg cctgtctcga cgaagccgag gcctctcttc
      601 cccgctcttc cggtatgtgg gagaatcttc ttcgcttgcg aaaggagctt gaggagctat
      661 gagcagagtg cggataaatg gggtacggct tgcatgctgt ctgtcgctgc ttctgtcggc
      721 tctgtttttt cccctctctt cctcccttgc atcggaggac gcaagcctgc tgaaggcggc
      781 ggagagggtc gcttcgggaa aagggagctc tgccgacgag gcgctcctct tcatggagaa
      841 ccggcgggtg aaccatctcg cgatggaggg gaagctggac gggagcgtct atcaaaagac
      901 gcaggagctc tacgacggaa agaacagggc gctggccgcg gaagccgcgc gcgaagcggg
      961 attcgccacg gaggccggcg gtaagaaaat ctacaagccc ggaacggaca ccgatatcca
//
LOCUS       DAOTIL010001105         1018 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminobacteriaceae bacterium isolate AMR_MDS_3026
            NODE_180565_length_1018_cov_2.066459, whole genome shotgun
            sequence.
ACCESSION   DAOTIL010001105 DAOTIL010000000
VERSION     DAOTIL010001105.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868050
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminobacteriaceae bacterium (wastewater metagenome)
  ORGANISM  Aminobacteriaceae bacterium
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae.
REFERENCE   1  (bases 1 to 1018)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1018)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:19:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,224
            CDSs (total)                      :: 3,198
            Genes (coding)                    :: 3,184
            CDSs (with protein)               :: 3,184
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1018
                     /organism="Aminobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_180565_length_1018_cov_2.066459"
                     /isolate="AMR_MDS_3026"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:3034609"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(13..636)
                     /locus_tag="P5201_16020"
     CDS             complement(13..636)
                     /locus_tag="P5201_16020"
                     /inference="COORDINATES: protein motif:HMM:NF037627.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRW00109.1"
                     /translation="MLESLSRVLLLDRQERLHLYHLANQAVPTDIPEYAGTVSPALQH
                     VLDSLRLCPSLVVDRCWNVLAWNEESCALFGDFSRMNARLRNVVWAVFTEPRYKNLLV
                     DWEANAKALLGRFRTVCGKLAEDPWLVRFVGDLKEQSEEFSLWWPLHEIHGDNEVRKK
                     LDHPVAGILDFEVNLFDVGDAPGLKLIVHTPLPGTETAEKMHSLLNA"
     gene            675..>1018
                     /locus_tag="P5201_16025"
     CDS             675..>1018
                     /locus_tag="P5201_16025"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRW00110.1"
                     /translation="MCTMLALFLRAERLPLSAIRTCVVAAPPEGGESTGGTGQPPASA
                     GTENAGDPIDWEAKYKEALGHSRDWEKKAKANKAAADELEKLKESQMSEAEKAAKRTQ
                     ELEAQVAAYKAK"
BASE COUNT          209 a          317 c          316 g          176 t
ORIGIN      
        1 cccatgcgat ttttacgcgt tcagaaggga gtgcattttt tcagcggtct ccgttcccgg
       61 aaggggcgta tggactatca gcttcaagcc gggggcgtcg ccgacgtcga agaggtttac
      121 ctcgaaatcc aggattcccg cgacggggtg gtccagtttt ttacgcactt cgttgtctcc
      181 gtggatctca tgcagcggcc accacaggga gaattcctcg ctctgttctt tcaaatcgcc
      241 gacgaaccgg acaagccacg ggtcttcggc gagcttcccg cagactgttc ggaatcttcc
      301 cagcagcgcc ttggcgtttg cttcccagtc gacaagcaga tttttgtacc ggggttccgt
      361 gaacacagcc cacacgacgt tgcgcagcct ggcgttcatc ctgctgaagt cgccgaagag
      421 ggcgcatgac tcctcgttcc aggcgaggac gttccagcac cggtcgacga cgagcgacgg
      481 gcagaggcgc aagctgtcca gaacatgctg caacgccggg ctcactgtcc cggcgtactc
      541 cggaatgtcc gtcgggacgg cctggttcgc cagatggtag aggtggaggc gctcctgcct
      601 gtcgagcaga agaactctgg agaggctctc cagcacctgc gccgagacgc ggatggggcg
      661 tccctgttca agccatgtgt accatgttag ccctattcct gcgagctgag cgacttcctc
      721 tctccgcaat ccggacgtgc gtcgtcgcgg ccccgccgga aggcggcgag tccaccggcg
      781 gcaccgggca accgccggca tcggccggca cggagaacgc cggcgatccg atcgactggg
      841 aagccaaata caaggaagcg ctcggccact cgcgcgactg ggaaaagaag gccaaggcca
      901 acaaggccgc agccgacgag ctggaaaagc tcaaggaatc ccaaatgagc gaagccgaga
      961 aggccgccaa gcgcacgcag gaactcgaag cgcaggtcgc cgcctacaag gccaagga
//
LOCUS       DAOTIL010001106         1015 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminobacteriaceae bacterium isolate AMR_MDS_3026
            NODE_181334_length_1015_cov_2.334375, whole genome shotgun
            sequence.
ACCESSION   DAOTIL010001106 DAOTIL010000000
VERSION     DAOTIL010001106.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868050
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminobacteriaceae bacterium (wastewater metagenome)
  ORGANISM  Aminobacteriaceae bacterium
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae.
REFERENCE   1  (bases 1 to 1015)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1015)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:19:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,224
            CDSs (total)                      :: 3,198
            Genes (coding)                    :: 3,184
            CDSs (with protein)               :: 3,184
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1015
                     /organism="Aminobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_181334_length_1015_cov_2.334375"
                     /isolate="AMR_MDS_3026"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:3034609"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..427)
                     /locus_tag="P5201_16030"
     CDS             complement(<1..427)
                     /locus_tag="P5201_16030"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRW00111.1"
                     /translation="MTWMVARAAAQQTGFPAAVKRCAGASGDLRDDTQAHVRRPVDFD
                     EIPRLHPFEVGAYLLRVFEDDARHSLAPPLGSVVAPGHPAGEHFDALCPAVCPGDDGL
                     DGLPDFVKRNPQRRGDHAHRLHEADVPDDASVTPSGVEFV"
     gene            417..800
                     /locus_tag="P5201_16035"
     CDS             417..800
                     /locus_tag="P5201_16035"
                     /inference="COORDINATES: protein motif:HMM:NF012428.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class III-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HRW00112.1"
                     /translation="MQVIREEQLMENALAVGGHIKNRFLEMQKKYEIIGDVRGIGLSI
                     GVDLVRNRETKERHGEAAAKICYRAWEKGLLLSFFSGSVLRIQPPLVISKEQADAALD
                     IIEESFQDFFAGDIPDSIFETVKGW"
BASE COUNT          190 a          325 c          333 g          167 t
ORIGIN      
        1 cgacgaattc gacgccggaa ggcgtgacgg aagcgtcgtc gggcacatcg gcttcgtgca
       61 gtcggtgagc atgatcgcct cgtcgctggg gattccgctt gacgaaatcc gggagaccat
      121 cgagcccatc gtctcccgga cacaccgccg gacagagagc gtcgaagtgc tccccggcag
      181 ggtggccggg tgcaaccacc gagccgaggg gtggagcaag ggaatgccgc gcatcgtcct
      241 cgaacacccg cagcaggtac gccccgacct cgaaggggtg gagacggggg atttcatcga
      301 aatcgacggg acgccggaca tgcgcctggg tatcgtcccg gagatccccg gaggccccgg
      361 cgcacctctt caccgcggcg ggaaaccccg tctgctgcgc cgccgccctg gccaccatcc
      421 aggtcatccg cgaagagcag ctgatggaga acgcgctcgc cgtcggcgga catatcaaga
      481 accgcttcct cgaaatgcag aagaagtacg agatcatcgg cgacgtgcgc ggcatagggc
      541 tctccatcgg cgtcgacctc gtcaggaacc gggagacgaa ggagcgccac ggcgaggctg
      601 ccgcgaagat ctgttaccgc gcgtgggaga aggggctgct gctctccttc ttcagcggca
      661 gcgtcctgag aatccagcct ccgctggtca tctcgaagga gcaggcggac gccgcgctcg
      721 acatcatcga ggagtcgttc caggacttct tcgccggcga catccccgac agcatcttcg
      781 agaccgtcaa ggggtggtag acaaactccg gaacgcctcc ggtaatcgca gggggcgttc
      841 gggagacaga aaggcagggg atgccgatgt caaaaatgtc ccttccggtg ttactgcttc
      901 tggcgaacgg cctgctcgcc ggcctgctca tagctctcgg cggtgcgacg cggagcgcct
      961 ttttcttctt cctgctcgcc atgctcctct gggcgtcgtc gctcatggcg tcgac
//
LOCUS       DAOTIL010001107         1015 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminobacteriaceae bacterium isolate AMR_MDS_3026
            NODE_181386_length_1015_cov_2.038542, whole genome shotgun
            sequence.
ACCESSION   DAOTIL010001107 DAOTIL010000000
VERSION     DAOTIL010001107.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868050
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminobacteriaceae bacterium (wastewater metagenome)
  ORGANISM  Aminobacteriaceae bacterium
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae.
REFERENCE   1  (bases 1 to 1015)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1015)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:19:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,224
            CDSs (total)                      :: 3,198
            Genes (coding)                    :: 3,184
            CDSs (with protein)               :: 3,184
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1015
                     /organism="Aminobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_181386_length_1015_cov_2.038542"
                     /isolate="AMR_MDS_3026"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:3034609"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..946)
                     /locus_tag="P5201_16040"
     CDS             complement(<1..946)
                     /locus_tag="P5201_16040"
                     /inference="COORDINATES: protein motif:HMM:NF018513.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0031317 - tripartite ATP-independent
                     periplasmic transporter complex [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter large permease"
                     /protein_id="HRW00113.1"
                     /translation="MTPMLIVGFFVLLIFLGVPIAFVIGIISLAGIATLPFVPNLTVF
                     MKMFNGLNSFVLLAVPLFILAANLMNHGQITRKLVNFCIALVGNIRGGLAHANILVSM
                     IFAGVSGSSQADTAGVGKMLIPAMIESGYDKENSVGVTAASSTIGVIIPPSIPMVIYS
                     GLTNASVAALFLGGMIPGILVGVTMMMIVYLIGHRRNFPKYEKAKYSDVWKLFKESSP
                     ALLTPVIIVGGITTGWYTPTEAAAFASVYALLISLFIYKTLKLRDLPGILKETLKLSS
                     LSLFALATASALGELLGYYKASEGVAAFFAANVGNPRIF"
BASE COUNT          225 a          266 c          340 g          184 t
ORIGIN      
        1 ggaagatacg aggattgccg acgtttgccg cgaagaaagc cgcgacgcct tcggaagcct
       61 tgtagtagcc aagcagttcg ccgagagcgc tggccgtggc aagcgcgaag agcgagagcg
      121 agctgagctt cagcgtctcc ttcaggattc cgggtagatc gcgcagtttc agcgttttgt
      181 atataaaaag gctgatgaga agggcataga cggaggcgaa ggccgccgcc tccgtgggcg
      241 tgtaccatcc tgtggtgatg ccgccgacga tgatgacagg ggtcagcagt gccggtgacg
      301 attccttgaa gagcttccag acgtcggagt acttcgcctt ctcgtacttc gggaagttcc
      361 tccggtggcc gataaggtag acgatcatca tcatggtgac gccgacgagg atgccgggga
      421 tcatccctcc gagaaagagc gccgcgacag aggcgttggt gaggccggag tagatcacca
      481 tcgggatgct cggcgggatg atgacgccga tggtcgagga cgccgcggtg acgccgacgg
      541 agttctcttt gtcgtacccg ctctcgatca ttgcgggaat cagcatcttc ccgacgcccg
      601 ccgtgtccgc ctgggaggag ccggagactc cggcgaagat catggacacg aggatattcg
      661 cgtgcgcgag gccgccgcgg atgttcccca caagcgcgat gcagaagttt accagcttcc
      721 gggtgatctg tccgtggttc atcaggttgg ctgccaggat gaagagcggt acggcgagca
      781 acacaaacga gttgagaccg ttgaacatct tcatgaagac ggtgaggttc gggacgaacg
      841 gcagcgtggc gatccccgcg agcgagatga tgccgatgac gaaggcgata ggcacgccga
      901 ggaagatgag cagcacgaag aacccgacga tcagcatcgg cgtcatgaca cggcctcctt
      961 cgaggagaga atacgtatgt catcaatgat gtgcgcgaca gtgtagaccg tcatc
//
LOCUS       DAOTIL010001108         1014 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminobacteriaceae bacterium isolate AMR_MDS_3026
            NODE_181587_length_1014_cov_2.686131, whole genome shotgun
            sequence.
ACCESSION   DAOTIL010001108 DAOTIL010000000
VERSION     DAOTIL010001108.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868050
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminobacteriaceae bacterium (wastewater metagenome)
  ORGANISM  Aminobacteriaceae bacterium
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae.
REFERENCE   1  (bases 1 to 1014)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1014)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:19:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,224
            CDSs (total)                      :: 3,198
            Genes (coding)                    :: 3,184
            CDSs (with protein)               :: 3,184
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1014
                     /organism="Aminobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_181587_length_1014_cov_2.686131"
                     /isolate="AMR_MDS_3026"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:3034609"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..390
                     /locus_tag="P5201_16045"
     CDS             <1..390
                     /locus_tag="P5201_16045"
                     /inference="COORDINATES: protein motif:HMM:NF014693.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FmdE family protein"
                     /protein_id="HRW00114.1"
                     /translation="WLKIVDTGRFAGVFYDKQTGEGVRISLSMERLKLWPRVEEWYLK
                     LTPKHEQSLQAILDEINEAGAELFDMVEVTVKPEVLKVRPKVPPVFCPICGEAYPPDH
                     GPICRGCSGLTDSYYHSRTPAAAGAER"
     gene            complement(470..>1014)
                     /locus_tag="P5201_16050"
     CDS             complement(470..>1014)
                     /locus_tag="P5201_16050"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRW00115.1"
                     /translation="GIRQVEERAQGVSVGEKRFGVLVAQRDQPILEGRNELSEVGGVP
                     HLLRYRTGDRGRFLSGTCRCGSILKRIETTGRLRNGITLHNGVFVPLHELDEALFSIP
                     WLADYSASQEDGYITVSLHAPSREATTPEPAPQEHAALAAALDNLSADAKSALWKRPF
                     RFRFLPHTHDGVSSRKRVAG"
BASE COUNT          179 a          307 c          338 g          190 t
ORIGIN      
        1 tggctgaaga tcgtggatac aggacggttc gccggcgtct tttacgacaa gcagaccggc
       61 gagggggtcc gcatctcgct gagcatggag cggctgaagc tctggccgcg cgtcgaggag
      121 tggtacctga agctcactcc gaaacatgag cagagccttc aggcgatcct cgacgagatc
      181 aacgaggcgg gcgccgaact cttcgacatg gtcgaagtaa ccgtgaaacc ggaagtgctc
      241 aaggtgcgcc cgaaggtccc gccggtcttc tgcccgatct gcggcgaggc ctacccgccc
      301 gaccacggcc cgatctgccg ggggtgttcg ggactcaccg actcctacta ccacagccgc
      361 acgcccgcag cggcaggcgc cgaacgctga acacacgaag agggagggct cgccgagccc
      421 tccctcttcg tgtcttcctt ccttttgttt cttgaagaag tcacacctct catcccgcga
      481 ctcgtttcct gctggagacg ccgtcgtggg tatgggggag aaagcggaag cggaacgggc
      541 gtttccacag ggcggacttc gcatcggctg agagattgtc gagcgcggcg gcgagcgctg
      601 cgtgttcctg cggggcgggt tccggcgtgg ttgcttctct cgaaggggcg tggagggaga
      661 ccgtaatgta tccgtcttcc tgcgacgccg agtagtcggc cagccagggg atggagaaga
      721 gcgcttcgtc cagctcgtgc agcggaacga agacgccgtt gtggagggtg atgccgtttc
      781 ggagtctccc cgtcgtttcg attcgtttga ggatggagcc gcagcggcac gttccggaga
      841 gaaagcgccc cctgtcgccg gtgcggtagc ggagaaggtg cggaacgcct ccgacttcgg
      901 aaagttcatt ccggccttcg agtatcggct gatctcgctg cgcgacaaga accccgaagc
      961 gcttctctcc tacggagacg ccctgggcgc gctcttctac ctggcgaatc cctc
//
LOCUS       DAOTIL010001109         1011 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminobacteriaceae bacterium isolate AMR_MDS_3026
            NODE_182438_length_1011_cov_2.869247, whole genome shotgun
            sequence.
ACCESSION   DAOTIL010001109 DAOTIL010000000
VERSION     DAOTIL010001109.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868050
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminobacteriaceae bacterium (wastewater metagenome)
  ORGANISM  Aminobacteriaceae bacterium
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae.
REFERENCE   1  (bases 1 to 1011)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1011)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:19:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,224
            CDSs (total)                      :: 3,198
            Genes (coding)                    :: 3,184
            CDSs (with protein)               :: 3,184
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1011
                     /organism="Aminobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_182438_length_1011_cov_2.869247"
                     /isolate="AMR_MDS_3026"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:3034609"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..319)
                     /locus_tag="P5201_16055"
     CDS             complement(<1..319)
                     /locus_tag="P5201_16055"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: protein motif:HMM:NF012492.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HRW00116.1"
                     /translation="MEINGIPHDEEIRYDSLGLSEPIMKALAKKGYEIATPVQGGAIP
                     FLMDRKDVIAKAPTGTGKTYAFGIPIIEHLRLEDEYVQALILAPTRELAIQIGEELRD
                     LSLF"
     gene            471..839
                     /locus_tag="P5201_16060"
     CDS             471..839
                     /locus_tag="P5201_16060"
                     /inference="COORDINATES: protein motif:HMM:NF012610.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HRW00117.1"
                     /translation="MDITFDPALPIYAQIVESFKREICSGVLRPGERVMPVRELALKL
                     GVNPNTLQRALSELEREGLLYTERTAGRFVTQDEALIAEVREELLSGIVMDVLRKATA
                     LGCPPEALADLIRNGGNPNG"
     gene            832..>1011
                     /locus_tag="P5201_16065"
     CDS             832..>1011
                     /locus_tag="P5201_16065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002935839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HRW00118.1"
                     /translation="MAEPILTLTDVSKQYGKQEALRHVSLAVEPGRIVGLLGPNGSGK
                     TTLIKIVNGLLRDYEG"
BASE COUNT          214 a          283 c          309 g          205 t
ORIGIN      
        1 tgaagagcga gaggtcgcgc agctcctcgc cgatctggat ggccagctcc cgcgtcggtg
       61 cgaggatgag cgcctggacg tattcgtctt ccagacgcag gtgttcgatg atgggaatgc
      121 cgaaggcgta agttttgccg gtccccgtgg gggctttggc gatgacgtct ttccggtcca
      181 tcagaaacgg gatcgcaccg ccctgcacgg gcgtggcgat ctcgtacccc tttttggcaa
      241 gcgctttcat aatgggctcc gagagcccga ggctgtcgta gcgtatctct tcgtcgtgcg
      301 ggattccgtt gatctccatt tgctcaatcc tcttactttg aaatcagatg atatcactat
      361 accatacccg aataaaaatt acaaatttaa taaaatgatc ttgacaaggc gcataaactg
      421 tattattgta ctagatggtt aatacaataa tacaaaatgg aggtgccggg atggacatca
      481 ctttcgaccc cgcgcttccc atctacgcac agatcgtcga gagcttcaag cgggagatct
      541 gcagcggcgt ccttcgcccc ggcgaacggg tgatgccggt gcgcgaactg gcgctgaagc
      601 tcggggtgaa cccgaacacg ctccagcgcg cgctctcgga gctcgaacgc gagggactgc
      661 tctatacgga gcggacggcc ggccgcttcg tgacgcagga cgaagcgctt atcgcggagg
      721 tccgggagga gctgctttcg gggatcgtca tggatgtgct gcgcaaggcg acggcgctgg
      781 gctgcccgcc cgaagcgctc gcggacctca tcagaaacgg agggaatccc aatggctgag
      841 cccattctca cgcttacgga cgtaagcaag caatacggga agcaggaggc gctccggcac
      901 gtgtctctcg ctgtggaacc cggacgcatc gtgggccttc tgggcccgaa cggcagcggc
      961 aagacgacgc tcatcaagat cgtcaacggc cttctcagag actacgaggg g
//
LOCUS       DAOTIL010001110         1011 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminobacteriaceae bacterium isolate AMR_MDS_3026
            NODE_182452_length_1011_cov_2.764644, whole genome shotgun
            sequence.
ACCESSION   DAOTIL010001110 DAOTIL010000000
VERSION     DAOTIL010001110.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868050
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminobacteriaceae bacterium (wastewater metagenome)
  ORGANISM  Aminobacteriaceae bacterium
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae.
REFERENCE   1  (bases 1 to 1011)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1011)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:19:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,224
            CDSs (total)                      :: 3,198
            Genes (coding)                    :: 3,184
            CDSs (with protein)               :: 3,184
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1011
                     /organism="Aminobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_182452_length_1011_cov_2.764644"
                     /isolate="AMR_MDS_3026"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:3034609"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            174..629
                     /gene="fliJ"
                     /locus_tag="P5201_16070"
     CDS             174..629
                     /gene="fliJ"
                     /locus_tag="P5201_16070"
                     /inference="COORDINATES: protein motif:HMM:TIGR02473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009288 - bacterial-type flagellum
                     [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0003774 - cytoskeletal motor activity
                     [Evidence IEA];
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar export protein FliJ"
                     /protein_id="HRW00119.1"
                     /translation="MKEKVDRFERILKTRVKGREEEQLLLAGQKSEEEQIVTRLDTLR
                     SQKERALVSFSMQQGTLISPQDMWFQRKSIDVIEKHICDGDSSLCDVRQEIEATEVRL
                     VEKHREVQIMEKYISSIVEEWRTTLQKNEQTEMDDIAGIRHGSHGRKNQ"
     gene            626..>1011
                     /locus_tag="P5201_16075"
     CDS             626..>1011
                     /locus_tag="P5201_16075"
                     /EC_number="4.2.2.n1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009201687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lytic transglycosylase domain-containing
                     protein"
                     /protein_id="HRW00120.1"
                     /translation="MRPNLSGIVRVQGRIEEIERRIRPKKELPEPPKQERFVDVLERE
                     EAKLPLPGASSGVRVPEVSEGWESHLPGLAARYGVDEALVRAVIRMESGGRTDAVSYK
                     GAMGLMQLMPGTAKMLGVEDPFDPVQ"
BASE COUNT          274 a          236 c          315 g          186 t
ORIGIN      
        1 aacgcatctg cagaaggtcc gcgcctttct tcgccaatca gtaggggaac atgtcccttt
       61 cgacgtcatg gcgaaggagc tgaagaacct cgtaccgtga ggccgcgaag aatcaagaaa
      121 aagtcactga gtgccgaaac atggaatgag aatgatgtat tccggaggac gccatgaagg
      181 aaaaagtgga tagatttgaa agaatactga agaccagagt aaaaggccgg gaagaggaac
      241 agctgcttct cgcgggccaa aagagcgaag aggagcagat tgtaacgcgt ctcgatacgc
      301 tccgctccca aaaggagcgg gcgctcgtct ccttcagtat gcagcaggga accttgattt
      361 ccccccagga tatgtggttc caacggaagt cgatagatgt catagaaaag catatctgcg
      421 acggcgactc ctccctctgc gatgtccggc aggaaataga agcgacggag gtgcgacttg
      481 tggagaaaca tcgcgaggtg cagattatgg aaaaatatat ctcatcgata gttgaggagt
      541 ggcggacaac gcttcagaag aacgagcaga ccgaaatgga tgatattgcc ggcattcgtc
      601 acggctctca cgggaggaag aatcaatgag accgaatctc tcgggtattg ttcgcgttca
      661 gggaagaatc gaggagatcg aacgccggat acgcccaaaa aaggagcttc cggagcctcc
      721 gaagcaggaa cgttttgttg atgtgctcga acgcgaggag gcaaagcttc cccttccggg
      781 cgcgtccagc ggagtgcgag ttcccgaagt ctccgaaggt tgggaatcac acctccccgg
      841 tttggcagcc cggtacggag tcgacgaagc cttggtacgc gccgtcatca ggatggaatc
      901 gggcggaagg acggacgccg tctcgtacaa gggggcaatg gggttgatgc agcttatgcc
      961 ggggacggcg aagatgctcg gggtagaaga tccgttcgat ccggtacaga a
//
LOCUS       DAOTIL010001111         1011 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminobacteriaceae bacterium isolate AMR_MDS_3026
            NODE_182536_length_1011_cov_2.020921, whole genome shotgun
            sequence.
ACCESSION   DAOTIL010001111 DAOTIL010000000
VERSION     DAOTIL010001111.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868050
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminobacteriaceae bacterium (wastewater metagenome)
  ORGANISM  Aminobacteriaceae bacterium
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae.
REFERENCE   1  (bases 1 to 1011)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1011)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:19:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,224
            CDSs (total)                      :: 3,198
            Genes (coding)                    :: 3,184
            CDSs (with protein)               :: 3,184
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1011
                     /organism="Aminobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_182536_length_1011_cov_2.020921"
                     /isolate="AMR_MDS_3026"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:3034609"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..713
                     /locus_tag="P5201_16080"
     CDS             <1..713
                     /locus_tag="P5201_16080"
                     /EC_number="3.4.13.-"
                     /inference="COORDINATES: protein motif:HMM:NF015536.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016805 - dipeptidase activity [Evidence
                     IEA];
                     GO_function: GO:0070004 - cysteine-type exopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="C69 family dipeptidase"
                     /protein_id="HRW00121.1"
                     /translation="SWDPYAETDSYPSSFAPEQPLSVKGIMEIFRSTMEGTPFCMEDN
                     PAWYVPRGNRELVRSAKATPFPDRFMRDLLNIPYTRPVAAKTSYSFITQSRSWLPSPL
                     GGVLWYSLDTPHFSTYAPVYAGVRSVPESWSNFKKDGFTLDSARWAVLLSSSLTNSNY
                     QRSMPTLKSLRDPLEKKTFHDFLQWEKEGSHLLLHSSPELQEWMDAITAKKMEEIHQT
                     YRSIAQALIMEATIIDLW"
     gene            700..>1011
                     /locus_tag="P5201_16085"
     CDS             700..>1011
                     /locus_tag="P5201_16085"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRW00122.1"
                     /translation="MICGKRTAVRSLSILGLFLLLSVRTVYAEQAMLVGSDAGRTYHV
                     LCLDAGAPYIFRAENGDLQGVYVDILSALGEREGLKLSLSLGDTHDARRGIHLSRIDF
                     IV"
BASE COUNT          233 a          292 c          253 g          233 t
ORIGIN      
        1 gaagctggga cccctacgct gagaccgact cctatccgtc ttctttcgct cctgaacaac
       61 cgctctccgt aaaagggata atggagatct ttcgctcgac aatggagggc accccgttct
      121 gtatggagga caaccccgca tggtatgtcc ctagaggaaa ccgggagctt gtccgcagtg
      181 caaaagccac tcctttcccc gacaggttca tgcgcgacct cttgaatatt ccttacacac
      241 gcccggtcgc ggcaaaaacg tcctacagct ttatcacaca atcccgttca tggcttccat
      301 ctccgctcgg aggcgttctc tggtattcac tcgacacccc gcatttcagc acatacgccc
      361 ccgtgtacgc cggagtgcgc agtgtgccgg aaagctggtc gaacttcaaa aaagatggat
      421 tcactcttga ttcggcgcgg tgggcggtgc ttctttcgtc atcacttacc aacagcaact
      481 atcagaggtc gatgccgacg ttgaaatcac tgcgcgatcc tctggaaaaa aagacgttcc
      541 acgactttct ccaatgggaa aaggagggtt cccatttgct ccttcactcc tcacctgaac
      601 ttcaggaatg gatggatgcc atcaccgcga aaaagatgga ggaaatacac caaacgtaca
      661 ggagcattgc gcaggctctt attatggagg caacaattat tgatctgtgg taaaagaacg
      721 gccgttcgtt cactctcgat tctcggactt tttctcctgc tctccgtccg taccgtatac
      781 gccgaacagg cgatgttggt aggaagcgac gcggggcgca cgtaccatgt cttgtgcctg
      841 gacgcaggag ccccctatat cttcagggcg gagaacggag atcttcaagg agtctatgtg
      901 gacatactgt ccgctctggg ggagagagaa gggttgaagc tctccctctc cctaggcgat
      961 acacacgatg cccgccgggg catccacctc tcccggattg atttcatcgt a
//
LOCUS       DAOTIL010001112         1010 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminobacteriaceae bacterium isolate AMR_MDS_3026
            NODE_182738_length_1010_cov_2.861780, whole genome shotgun
            sequence.
ACCESSION   DAOTIL010001112 DAOTIL010000000
VERSION     DAOTIL010001112.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868050
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminobacteriaceae bacterium (wastewater metagenome)
  ORGANISM  Aminobacteriaceae bacterium
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae.
REFERENCE   1  (bases 1 to 1010)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1010)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:19:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,224
            CDSs (total)                      :: 3,198
            Genes (coding)                    :: 3,184
            CDSs (with protein)               :: 3,184
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1010
                     /organism="Aminobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_182738_length_1010_cov_2.861780"
                     /isolate="AMR_MDS_3026"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:3034609"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..506
                     /locus_tag="P5201_16090"
     CDS             <1..506
                     /locus_tag="P5201_16090"
                     /inference="COORDINATES: protein motif:HMM:NF024206.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ankyrin repeat domain-containing protein"
                     /protein_id="HRW00123.1"
                     /translation="DYAGLTPLMWAAARNEEPGIAALLLQRGAEVNARDKNGVTVLMW
                     AAANNLNPEIVSVLAKAGAYPDARGKNGDTAIMGAAMSNPNPQVVAALVEAGADVNMK
                     GPRDWTALMFGALHTRNPEVVSSLLAAGANATIQNRDGGRAIDYAGKNPHLKGTKAYW
                     LLNEASY"
     gene            complement(531..>1010)
                     /locus_tag="P5201_16095"
     CDS             complement(531..>1010)
                     /locus_tag="P5201_16095"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HRW00124.1"
                     /translation="AGYAVDEEVEETIDASRHAPSESILLAIHTPERPTVLIVEDQRL
                     NRMLLKKLVDALLPSATVYEAVDGVEGVELFERHAPDIVFMDLQMPRKDGFHAATEIR
                     ALEADGRRTLIIALTADAQPETRESCLESGMDDYLTKPVSIHDLRRVLEHGAMRTEE"
BASE COUNT          197 a          292 c          334 g          187 t
ORIGIN      
        1 gggattacgc agggctgacg ccgctcatgt gggcggcggc gcggaacgag gaacccggaa
       61 ttgcggcgct tctgttgcaa cggggcgcgg aggtgaacgc cagggacaag aacggcgtca
      121 cggtgctgat gtgggctgct gcgaacaacc tgaatccaga aattgtgagc gtgttggcga
      181 aagccggggc gtatccggac gcgaggggga agaacggcga cacggccatc atgggagcgg
      241 cgatgagcaa cccgaacccg caggtcgtcg ccgcgctcgt cgaggccggg gcggacgtga
      301 atatgaaggg accgcgcgat tggaccgcgc tgatgttcgg ggcgctgcac acccggaacc
      361 ctgaagtggt gagctcgttg ctggcggcgg gggcgaacgc gacgatacaa aacagggacg
      421 gcggacgcgc catcgactac gccggaaaaa atccgcacct caagggaacc aaggcatact
      481 ggcttctgaa cgaggcctcg tactgagagg cctcgtactg aatcgaagga ttattcctcc
      541 gtcctcatcg ctccatgttc caagactctc cggagatcgt ggatgctcac cggcttggtg
      601 aggtaatcgt ccattcctga ttcgaggcag ctctctcttg tctccggctg ggcgtcggca
      661 gtgagcgcga tgatcaatgt tctccgtccg tccgcttcca gagcccgtat ctccgtcgcg
      721 gcatggaaac cgtcctttcg gggcatctgc agatccataa agacgatgtc cggagcgtgg
      781 cgttcgaaga gctccactcc ctccaccccg tcgacggcct cgtagacggt tgccgacggc
      841 agcaacgcgt cgaccagttt tttcaacagc atccggttga gccgctgatc ttcgacgatg
      901 agcaccgtag gtcgctccgg ggtgtgaatc gcgagcagaa tactttccga gggggcgtgt
      961 cgcgaagcgt cgatggtttc ttcgacctcc tcgtcgactg cgtatccggc
//
LOCUS       DAOTIL010001113         1008 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminobacteriaceae bacterium isolate AMR_MDS_3026
            NODE_183322_length_1008_cov_3.311647, whole genome shotgun
            sequence.
ACCESSION   DAOTIL010001113 DAOTIL010000000
VERSION     DAOTIL010001113.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868050
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminobacteriaceae bacterium (wastewater metagenome)
  ORGANISM  Aminobacteriaceae bacterium
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae.
REFERENCE   1  (bases 1 to 1008)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1008)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:19:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,224
            CDSs (total)                      :: 3,198
            Genes (coding)                    :: 3,184
            CDSs (with protein)               :: 3,184
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1008
                     /organism="Aminobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_183322_length_1008_cov_3.311647"
                     /isolate="AMR_MDS_3026"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:3034609"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            76..>1008
                     /locus_tag="P5201_16100"
     CDS             76..>1008
                     /locus_tag="P5201_16100"
                     /inference="COORDINATES: protein motif:HMM:NF015859.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HRW00125.1"
                     /translation="MPSSSSYALSFPVLYSVSADRRILPPGTDLRSIPSLKHAIELSV
                     RNGMKTASEAATSDVGSDGACRSVLFRPVRSAGGDLMGVVGVVLNLEVLLNDTMLRDE
                     GAQQHVMDVELKRLLPGGEERLLLSMPSGADALPRERNAESFHSLRPFFVFGRSFVLE
                     TRSGEGFASMHPERMGKIVLFFGIAVSALITLLAGLFLNWRRTLLRFVTIKTVSLAQS
                     EFRSRKLAHLYRSITEGVVITDAEGLIEECNDALEELTGFALEEMQGRSLAIFSAREG
                     QYDHEFLRSLGQNGVWQGEVWNRKRDGEVYPVWLT"
BASE COUNT          171 a          297 c          320 g          220 t
ORIGIN      
        1 tctttagcag ccgaaagcgg cgacctgtcc gaagccgggc ttctctcttc cggggattcg
       61 gaggaaggac cttccatgcc ttcttcatcc tcctacgcgc tctccttccc ggtgctctat
      121 tcggtctccg ccgaccggag gatactgcct ccggggaccg atctgcgatc cattccctct
      181 ttaaaacatg ccattgagct ctctgtacga aacggcatga agaccgcctc cgaagcggcg
      241 acgagcgacg tcggttccga cggggcatgc cgttccgtcc ttttccgtcc tgtgcggagc
      301 gccgggggcg acctgatggg cgttgtcggc gtcgtgctca acctggaggt tttgctgaac
      361 gatacgatgc tgcgtgacga gggggcgcaa cagcacgtca tggatgttga actcaagcgc
      421 ctcctgcccg gcggggaaga gcgcctgttg ctctctatgc catcaggagc ggatgcgctt
      481 ccaagggaac ggaacgcgga gtcttttcac tctctgcgcc ccttcttcgt gttcggtcgg
      541 tcttttgtgc tggagacgcg ctcgggggaa gggtttgcct cgatgcaccc ggaacgaatg
      601 gggaagatcg ttctcttctt cggtatcgcc gtctcggcgc ttattacgct gctcgccggg
      661 ctctttctca actggcggcg gacgctgctc cgcttcgtga cgatcaagac ggtctctctg
      721 gcgcaaagcg aatttcgttc ccgcaagctc gcgcacctct accgctccat cacggaggga
      781 gtggtgataa cggatgcgga gggcctcatc gaggagtgca acgacgcgct cgaagagctg
      841 acgggcttcg cgcttgagga gatgcagggg agatcgcttg ccatcttctc cgccagggag
      901 gggcagtacg atcatgagtt cctgcgctcg ctcgggcaga acggggtatg gcaaggcgag
      961 gtgtggaacc ggaagaggga cggagaagta taccctgtct ggctcacc
//
LOCUS       DAOTIL010001114         1007 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminobacteriaceae bacterium isolate AMR_MDS_3026
            NODE_183618_length_1007_cov_3.554622, whole genome shotgun
            sequence.
ACCESSION   DAOTIL010001114 DAOTIL010000000
VERSION     DAOTIL010001114.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868050
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminobacteriaceae bacterium (wastewater metagenome)
  ORGANISM  Aminobacteriaceae bacterium
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae.
REFERENCE   1  (bases 1 to 1007)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1007)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:19:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,224
            CDSs (total)                      :: 3,198
            Genes (coding)                    :: 3,184
            CDSs (with protein)               :: 3,184
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1007
                     /organism="Aminobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_183618_length_1007_cov_3.554622"
                     /isolate="AMR_MDS_3026"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:3034609"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            192..479
                     /locus_tag="P5201_16105"
     CDS             192..479
                     /locus_tag="P5201_16105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005661359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BMC domain-containing protein"
                     /protein_id="HRW00126.1"
                     /translation="MNNGMQALGMIETKGLVGAIEAADAMVKAANVTLIGKVMVGGGL
                     VTVMVRGDVGAVKAATDAGAAAAGKVGELISVHVIPRPHGEVEFILPKLEG"
     gene            528..>1007
                     /locus_tag="P5201_16110"
     CDS             528..>1007
                     /locus_tag="P5201_16110"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006584216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PduL/EutD family phosphate acyltransferase"
                     /protein_id="HRW00127.1"
                     /translation="MNPETRPGQSSGKTPGMGAEALELLVGRVVAEVRKRLSFEVEAS
                     GRHVHLNREHVDALFGKGHTLTDSRGLSQPGQFLCSERVTLAGPKGAIENVAILGPER
                     KQSQVELSITDATMLGVKAPVRDSGDLEGSAPLRLRTPKGEIALEKGVIVAQRHIHMT
                     "
BASE COUNT          192 a          287 c          369 g          159 t
ORIGIN      
        1 cgtgcgcgag atcgaggatc tgcgccccgc gaagcaggcg ggtcccgcga tgctcaacgg
       61 cgaccagctc gaccgtctgg tcgagcgcat cgtagaagag ttggctggga agcttaagct
      121 gtaaaaaaga gcgcgttaaa gcgcgcgaca ttctttgatt cacacatact tccgagggag
      181 gttgcagaga gatgaataac ggaatgcagg cgcttggaat gatcgaaacg aagggacttg
      241 tcggcgcgat cgaggctgcc gacgcaatgg tgaaggccgc gaacgtgacg cttatcggca
      301 aggtgatggt gggcggcggt ctcgtgaccg tgatggtgcg cggcgacgtc ggcgcggtga
      361 aggccgcgac ggacgcgggc gctgcggcgg ccgggaaggt cggggagctt atctccgtcc
      421 acgtgattcc gcgtccgcac ggcgaagtcg agttcattct tccgaagctg gaaggctaag
      481 tccggagccc cgtgctccgg agtcggcccc ggaagggagc ggcagccgtg aaccctgaaa
      541 cgcgtcccgg acagagttcc gggaaaacgc ccggaatggg cgctgaagcg cttgaactgc
      601 tcgtcgggcg ggtggtcgcc gaggtccgga agcgcctctc cttcgaggtg gaggcctccg
      661 gacggcatgt ccatctgaac cgcgagcatg tcgacgcgct cttcgggaag gggcataccc
      721 tcaccgactc gcgcgggctt tcgcagccgg ggcagttcct ctgctccgag cgcgtgacgc
      781 tggccgggcc gaagggcgcg attgaaaacg tagcgatact cggccccgag cggaagcaga
      841 gccaggtgga gctttccatt acggacgcga cgatgctcgg ggtgaaagcc cccgtccgcg
      901 actccgggga cctggagggg agcgcgccgc ttcggctgcg gactccgaag ggggagatcg
      961 cgctcgaaaa gggcgtgatc gtggcccagc ggcatatcca catgacg
//
LOCUS       DAOTIL010001115         1004 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminobacteriaceae bacterium isolate AMR_MDS_3026
            NODE_184469_length_1004_cov_3.202318, whole genome shotgun
            sequence.
ACCESSION   DAOTIL010001115 DAOTIL010000000
VERSION     DAOTIL010001115.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868050
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminobacteriaceae bacterium (wastewater metagenome)
  ORGANISM  Aminobacteriaceae bacterium
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae.
REFERENCE   1  (bases 1 to 1004)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1004)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:19:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,224
            CDSs (total)                      :: 3,198
            Genes (coding)                    :: 3,184
            CDSs (with protein)               :: 3,184
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1004
                     /organism="Aminobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_184469_length_1004_cov_3.202318"
                     /isolate="AMR_MDS_3026"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:3034609"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..963)
                     /locus_tag="P5201_16115"
     CDS             complement(<1..963)
                     /locus_tag="P5201_16115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013048936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase subunit E"
                     /protein_id="HRW00128.1"
                     /translation="MALYRAHVLICHGTGCASGGAPKVLEAFKEELAKKGLEKEILVI
                     ATGCHGMCEFGPIVVVYPEGTFYSQVKPSDVAEIVEEHLYKGRIVTRLLYKEPLSAQK
                     VCHYKDIPFYGKQHRIVLSNCGYINPDNIDEYISRDGYQALGKCLVEMTPAAVIDEMK
                     KSGLRGRGGGGFPTGMKWGFCAASPGDKKYVICNADEGDPGAFMDRSILEGDPHAVIE
                     GMMIGGYAMGADEGYIYCRAEYPLAIKRLQTAIKNATEYGLLGEDILGTGFNFTLHIK
                     EGAGAFVCGEETALMASIEGQRGMPRARPPFPAVKGLWGKPTNIN"
BASE COUNT          206 a          291 c          258 g          249 t
ORIGIN      
        1 gttgatattc gtcggcttac cccagagtcc tttgacagcc gggaaaggcg gacgggcgcg
       61 cggcatgcct ctctgtcctt ctatggaggc cataagcgcg gtctcttcgc cgcagacgaa
      121 agctccggcg ccctccttga tgtgaagggt gaagttgaag cccgttccta agatgtcttc
      181 gcccagcagg ccatattcgg tggcattctt tatagcggtc tgcaggcgtt taattgccag
      241 ggggtattca gcacgacaat agatgtatcc ttcgtcggcg cccatggcgt agccgccgat
      301 catcatacct tcgatgacgg cgtgggggtc gccctcaaga atggagcgat ccatgaacgc
      361 gcccggatct ccctcgtccg cattgcagat gacatatttc ttatcgccgg gggacgctgc
      421 gcagaaaccc catttcattc ctgtcgggaa accgccgcct ccacgccctc gaagcccgga
      481 ctttttcatc tcgtcaatca ccgctgccgg ggtcatctcg acaagacact ttcccagagc
      541 ttggtatccg tctctggaga tgtactcgtc aatattgtcc gggttgatat acccgcagtt
      601 gctcaatacg attctatgct gtttaccgta gaacggaata tccttgtagt gacagacctt
      661 ctgagcggac agaggttctt tgtaaagaag acgtgtgacg atgcggccct tatacagatg
      721 ctcttcgacg atttctgcaa cgtctgaagg cttaacctgc gagtaaaacg ttccttccgg
      781 atagaccacg acgatcggac cgaattcaca catgccgtgg cagccggttg caataacaag
      841 gatctctttt tcgagacctt tctttgcaag ctcttcttta aacgcttcaa gcaccttcgg
      901 ggcgccgccg gaagcgcatc ctgttccgtg acagatgagt acgtgcgcgc ggtacaatgc
      961 catatcctta tccctccccg cttagtcggc tctgccgtac agct
//
LOCUS       DAOTIL010001116         1004 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminobacteriaceae bacterium isolate AMR_MDS_3026
            NODE_184501_length_1004_cov_2.717597, whole genome shotgun
            sequence.
ACCESSION   DAOTIL010001116 DAOTIL010000000
VERSION     DAOTIL010001116.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868050
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminobacteriaceae bacterium (wastewater metagenome)
  ORGANISM  Aminobacteriaceae bacterium
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae.
REFERENCE   1  (bases 1 to 1004)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1004)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:19:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,224
            CDSs (total)                      :: 3,198
            Genes (coding)                    :: 3,184
            CDSs (with protein)               :: 3,184
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1004
                     /organism="Aminobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_184501_length_1004_cov_2.717597"
                     /isolate="AMR_MDS_3026"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:3034609"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>1004
                     /locus_tag="P5201_16120"
     CDS             <1..>1004
                     /locus_tag="P5201_16120"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HRW00129.1"
                     /translation="QVLVNLTNNAVKFTERGSVTLSVSFDPLKKNEGALTFRVTDTGI
                     GIPPEERKRVFEPFYQADNSNTRKYGGIGLGLSISQRILQMMGGSLEIHSVPGEGSSF
                     FFTLRLAYDAEEARSPAATPLPEEKRGFPHSRRKDTPVILLAEDERLNMKMLTIMLSK
                     LAPFSSVVQAEDGEKAVALFREHRPDLVLMDLQMPVIDGLQATAEIRAIEAKECREKE
                     RCCIVALTADVLPETRGECLAGGMDDYLSKPVRREEIMAVLERCFGMNGGGERDFRDA
                     PAEDAAWNRPLMLDVLDGDEALADEIASEFLKETPQMLAALSDALAEKYTEMFRFPEM
                     "
BASE COUNT          236 a          290 c          306 g          172 t
ORIGIN      
        1 agcaggtgct cgtcaatctg acgaacaacg cggtcaaatt cacggagcgg ggaagcgtca
       61 cgctctcggt cagcttcgat cctctgaaga aaaacgaagg cgccctcacc ttccgcgtga
      121 cggatactgg catcggaatc cctccggaag agcgaaaacg cgtcttcgaa cccttctacc
      181 aggccgacaa ctccaatacg cgcaagtacg gagggatcgg gctcggcctc tccatcagcc
      241 aacgtattct gcagatgatg gggggttcgc tcgaaataca cagcgtcccc ggagagggga
      301 gcagcttctt cttcacgctg cggctggcat acgacgcgga ggaagcgcgc tctccggcgg
      361 cgacgcccct tccggaagaa aaacggggat ttccccactc gcggagaaaa gacaccccgg
      421 tgatcctgct cgccgaggac gaacggctga atatgaagat gctcaccatc atgctgtcga
      481 agcttgctcc tttctcctcc gtagtacagg ccgaagacgg agaaaaagca gtcgccctct
      541 tccgcgaaca ccggccggac ctcgtcctga tggatcttca gatgccagtg atcgacggct
      601 tgcaagcaac ggcggagata cgcgcgattg aagcgaagga atgccgcgaa aaagagcgct
      661 gctgcatcgt cgccctcaca gcggacgttc tgccggaaac cagaggggag tgtctcgcgg
      721 gcggtatgga cgactatctg tcgaaacccg tacgcagaga agagatcatg gccgtcctcg
      781 aacgatgctt cggaatgaat ggaggcggag aacgagactt ccgggacgca cccgccgagg
      841 acgcggcctg gaacagaccg ctcatgctgg atgtgctcga cggcgacgaa gcgctggccg
      901 atgaaattgc gtcggagttt ttgaaggaaa cgccgcaaat gctggccgct ctcagcgatg
      961 cgctggcgga gaaatacact gagatgttcc ggtttccgga gatg
//
